LOCUS       DAEMIK010000001        61845 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEMIK010000001 DAEMIK010000000
VERSION     DAEMIK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61845
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.001"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1141
                     /locus_tag="KPK87_000001"
     CDS             <1..1141
                     /locus_tag="KPK87_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2536208.1"
                     /translation="NNFKMINDTFGYEFGDNLLITIAKALKEELTEGEVYARLSSDYF
                     AIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEGEVDIQKAVNKAN
                     MARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVYYQPKFALVTGEM
                     IGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCNDLYEWKKQGKKIV
                     PVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINELNILKNVLNELR
                     TYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSRNIAKSIVSLAKS
                     LDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            1395..2057
                     /locus_tag="KPK87_000002"
     CDS             1395..2057
                     /locus_tag="KPK87_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG2536209.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            2428..3837
                     /locus_tag="KPK87_000003"
     CDS             2428..3837
                     /locus_tag="KPK87_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG2536210.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            3952..4227
                     /locus_tag="KPK87_000004"
     CDS             3952..4227
                     /locus_tag="KPK87_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG2536211.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            4857..5168
                     /locus_tag="KPK87_000005"
     CDS             4857..5168
                     /locus_tag="KPK87_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536212.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            5170..7095
                     /locus_tag="KPK87_000006"
     CDS             5170..7095
                     /locus_tag="KPK87_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG2536213.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            7098..7589
                     /locus_tag="KPK87_000007"
     CDS             7098..7589
                     /locus_tag="KPK87_000007"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG2536214.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            7640..8203
                     /locus_tag="KPK87_000008"
     CDS             7640..8203
                     /locus_tag="KPK87_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG2536215.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            8218..9195
                     /locus_tag="KPK87_000009"
     CDS             8218..9195
                     /locus_tag="KPK87_000009"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG2536216.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            9182..9505
                     /locus_tag="KPK87_000010"
     CDS             9182..9505
                     /locus_tag="KPK87_000010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG2536217.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            9622..11400
                     /locus_tag="KPK87_000011"
     CDS             9622..11400
                     /locus_tag="KPK87_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG2536218.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            11397..12770
                     /locus_tag="KPK87_000012"
     CDS             11397..12770
                     /locus_tag="KPK87_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG2536219.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            12776..13444
                     /locus_tag="KPK87_000013"
     CDS             12776..13444
                     /locus_tag="KPK87_000013"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG2536220.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            13722..14771
                     /locus_tag="KPK87_000014"
     CDS             13722..14771
                     /locus_tag="KPK87_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2536221.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(15006..16364)
                     /locus_tag="KPK87_000015"
     CDS             complement(15006..16364)
                     /locus_tag="KPK87_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2536222.1"
                     /translation="MLRVALYIRVSTEEQALNGDSIRTQIEALEQYSKENDFNIVGKY
                     IDEGCSATNLKRPNLQRLLRDVEKDKVDLVLMTKIDRLSRGVKNYYKIMETLEKHKCD
                     WKTILENYDSSTAAGRLHINIMLSVAENEAAQTSERIKFVFQDKLRRKEVISGTIPIG
                     YKIENKHLVIDKEKIYIVKAIFDEYEKSGSVRTLIETINNLHGELYSYNKIKNILRNE
                     LYIGIYNKRGFYVEDYCEPIISKKQFKQIQRILEKNKKTTPNKNIHYHIFSGLLKCKE
                     CGYTLKGNSSNVGEKLYLSYRCSTFYLNKNCVHNVTHNEKHIENYLLTNLKPQLHKHM
                     VKLEAQNEKIRRNKKSNKKDEKKKIMKKLDKIKDLYLEDLIDKETYRKDYEKLQSQLD
                     NITEEQESQIIDTSHIKKFLDIDINEMYSDLSRVERRRFWLSIIDYIEIDNNKNITIN
                     FI"
     gene            complement(16429..16902)
                     /locus_tag="KPK87_000016"
     CDS             complement(16429..16902)
                     /locus_tag="KPK87_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2536223.1"
                     /translation="MNSYEKLLSEADDNNIIVREVPLISNSHGLYKNNRIALNKNTLN
                     NISEKACVLAEELGHHYTSYGNILDLNKVENSKQEYKARLMAYNKLIGLKGIIDSFNA
                     GCKTITEMAEYLDVTEKFLNEALECYKSKYGFSATLDNYVIFFEPRFSIMNANFL"
     gene            complement(16920..17375)
                     /locus_tag="KPK87_000017"
     CDS             complement(16920..17375)
                     /locus_tag="KPK87_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2536224.1"
                     /translation="MNTENDLKMLEITNRIKNKRLELNMSYQDLANKTGLSKSTLQRY
                     ETGAIKNIPLDKLEILAHALDESPAFIMGWNEDCKQIKIKENKLLSSFNALNDIGQNE
                     AIKRVDELTQIGKYVNKNHIDTIAAHNEHLHEEGEIEKIYQDLDDMDNW"
     gene            17529..17750
                     /locus_tag="KPK87_000018"
     CDS             17529..17750
                     /locus_tag="KPK87_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2536225.1"
                     /translation="MNISKLKGKMVEKKYTQKKLAEELGMTVQALNAKLNNRSQFKIE
                     EAVKISNILQIEEPGEIFFDDYIPKMQRN"
     gene            17798..18001
                     /locus_tag="KPK87_000019"
                     /pseudo
     CDS             17798..18001
                     /locus_tag="KPK87_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(18142..18315)
                     /locus_tag="KPK87_000020"
     CDS             complement(18142..18315)
                     /locus_tag="KPK87_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536226.1"
                     /translation="MATQKPRFTITVDDELLKEIDDFRFDKRFSTRTQATIELIKLGL
                     EKLNAEKCKETKE"
     gene            18471..18641
                     /locus_tag="KPK87_000021"
     CDS             18471..18641
                     /locus_tag="KPK87_000021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536227.1"
                     /translation="MPTKSKRISITIFPELETDLDVLKKEKFYKESQSEMLRYLIKLG
                     LQVNKEKVYKNE"
     gene            18658..19446
                     /locus_tag="KPK87_000022"
     CDS             18658..19446
                     /locus_tag="KPK87_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBG2536228.1"
                     /translation="MNDLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLRSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MERELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLGVI
                     ETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQISMLS"
     gene            19507..19695
                     /locus_tag="KPK87_000023"
     CDS             19507..19695
                     /locus_tag="KPK87_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536229.1"
                     /translation="MGKKSEYTVTTVGEPNLDALGETLAKILTEKFGVLITHTYIENY
                     YVDKERGGDVDGAIESEL"
     gene            19679..19898
                     /locus_tag="KPK87_000024"
                     /pseudo
     CDS             19679..19898
                     /locus_tag="KPK87_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861754.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19998..20315
                     /locus_tag="KPK87_000025"
     CDS             19998..20315
                     /locus_tag="KPK87_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536230.1"
                     /translation="MESLKIVKQYVEGQLNLSSLEIDKNKETYEILKNKSSRDMLDDI
                     NLNDALREVTVNERLKIFAESLLELLDTQIKIKESEESEDYKRLCMYLDEFGRDRPID
                     VQI"
     gene            20401..20910
                     /locus_tag="KPK87_000026"
     CDS             20401..20910
                     /locus_tag="KPK87_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG2536231.1"
                     /translation="MSTLYELTTDLLEIEEGLTEITGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVIIDIDSDINSLDLEIKRMQELKKIKKNSLDRLKSNIKECMELLGLKKVETFL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            20920..21528
                     /locus_tag="KPK87_000027"
     CDS             20920..21528
                     /locus_tag="KPK87_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG2536232.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDKVVQIGTRFYVEATATLIDAETGEKVSAKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNTHGKEQKKREVSEDELSTLYFLGESIDK
                     DKNRVDSEVYKKFSKLAIDLTKQEYEKVLNGYKSILDKQNQE"
     gene            21537..22487
                     /locus_tag="KPK87_000028"
     CDS             21537..22487
                     /locus_tag="KPK87_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG2536233.1"
                     /translation="MNFNNETYFHINFDDPFTRVPNTILDNENLSYSAIGVVTQMLRF
                     QRSGSHKVYAKSLISYRKDSKTKVSNALKELMQEGFVIRTQIRDEKGQMKGYRYDIFD
                     TPQNVNSESVETTESQPCAVFPTPVKPEAGKTEVGETGSRQNRGREIGNIKENSIKKK
                     IGLKENDVITTVIAEQSEKNKTVYIKKYYESYIGVITPNNFLQLLTYLDDGMEADVII
                     RAVDEAVGSGVKNYKYVKTILNNWIEAGVKTVLELTEYQNEFERKKKSKQEKKQSNSK
                     TVNTHNVNKNKFANFNQTFTQYEEKELDEIIKKSQKEKFK"
     gene            22548..22721
                     /locus_tag="KPK87_000029"
     CDS             22548..22721
                     /locus_tag="KPK87_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBG2536234.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            22737..23153
                     /locus_tag="KPK87_000030"
     CDS             22737..23153
                     /locus_tag="KPK87_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2536235.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKSDSNNHKEQQEYIPPGLDPQGFQAIDDDDIPF"
     gene            23227..23484
                     /locus_tag="KPK87_000031"
     CDS             23227..23484
                     /locus_tag="KPK87_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536236.1"
                     /translation="MKYECEKVFLECDKGSFEINDTRIGEVMFESTEIDNPFERVKYE
                     GTFEIVSGWEYLQREMLWLKILHLSAIVAKIMQYKMLGISK"
     gene            23496..23846
                     /locus_tag="KPK87_000032"
     CDS             23496..23846
                     /locus_tag="KPK87_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536237.1"
                     /translation="MLEKTRKNKISVCYQCRNEDISEDARYCKICGIGLRVMELVSVF
                     NFETGEKELSFLKGKECLIDFGDLREGNMCKLFFENTELRIIGVENIYQDKRGIFIEA
                     GECSHEIIFKDLEW"
     gene            23850..24230
                     /locus_tag="KPK87_000033"
     CDS             23850..24230
                     /locus_tag="KPK87_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536238.1"
                     /translation="MAKIWVDAGTFLEKTMDLEDMFELNLRKVRKANESKKIKLKLNE
                     SKFKKIRKKTTNDTKSKPIKVFNIETGEVRIFKSAKAASKYLKISGDYASCLARENRV
                     TREGWKAEYIQEVSDGISKCGTSN"
     gene            24199..24360
                     /locus_tag="KPK87_000034"
     CDS             24199..24360
                     /locus_tag="KPK87_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536239.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSRYEYEGML
                     RNESTNAVN"
     gene            24335..24493
                     /locus_tag="KPK87_000035"
     CDS             24335..24493
                     /locus_tag="KPK87_000035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536240.1"
                     /translation="MKVQMQLTKDKEFFKVYVNSEEEELERLFFEFVSQMLAYKRKNK
                     KVQGDIEK"
     gene            24490..24663
                     /locus_tag="KPK87_000036"
     CDS             24490..24663
                     /locus_tag="KPK87_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG2536241.1"
                     /translation="MKLKDIIKLGEKYCYCPNCGNDKVGNNEGKLIVEEHTYYRECSC
                     GFNILIDDRKDEI"
     gene            24663..24998
                     /locus_tag="KPK87_000037"
     CDS             24663..24998
                     /locus_tag="KPK87_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536242.1"
                     /translation="MNMLASVILVIGSFIAGRVYEYRLNLNENDEADSKVLLDVFNEI
                     SVLREENKSLKEKLQEKELLFINRLIDFLHDKKICECCIYDCKIDDIEYDCEDGIKKW
                     LDSEELIFE"
     gene            25106..26179
                     /locus_tag="KPK87_000038"
     CDS             25106..26179
                     /locus_tag="KPK87_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG2536243.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            26263..26958
                     /locus_tag="KPK87_000039"
     CDS             26263..26958
                     /locus_tag="KPK87_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536244.1"
                     /translation="MDLNKIMNDALVEIEESGFVEETVKKELEETIKRAVNEIFGSYS
                     KFRKNIEQHLGENININLDELDIQKYNLLVANVVKEKVDATMKGQGIEHLKRNLDNML
                     VGLKKEYKMSELLEELKEDKYNLDEYPCDEDCITFILESNFNSEWARWIDFDECPNVS
                     RNNCEHSILLRNDGTIAAFRYQNREISSKDIMNGFGNFGDLLFKIYAHNSKIILDLGN
                     DVDDYDLTNGEDY"
     gene            26974..27195
                     /locus_tag="KPK87_000040"
     CDS             26974..27195
                     /locus_tag="KPK87_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536245.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSIENLKKKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYYCDGGIEI"
     gene            27192..28196
                     /locus_tag="KPK87_000041"
     CDS             27192..28196
                     /locus_tag="KPK87_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2536246.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRIAVFNDYSNNLIKSCCEQIIYDESSFLNNSKEIASYLFEIMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNKSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLRVLDKNAEKEEANSKFVTEFLNATKVKDDKYKTKMFKSTAENWITNAL
                     GNDIKQAEDVRSILNYTLKEKHEIDINNFVDKTIKDDKLKDSFKEHMEEKGLDKGFSI
                     DKKWVEKKLKKRNIKTDNGFDIKGNLTVFEDPMKYTVRQNQNGSIDIIIKNVKFYEEK
                     "
     gene            28205..28372
                     /locus_tag="KPK87_000042"
     CDS             28205..28372
                     /locus_tag="KPK87_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536247.1"
                     /translation="MTNKEMCKLKNLDEREVYKEFGKEICGSCKNNKGDCESKNCSEA
                     CKNWLEKVCKS"
     gene            28369..28722
                     /locus_tag="KPK87_000043"
     CDS             28369..28722
                     /locus_tag="KPK87_000043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536248.1"
                     /translation="MTELGELLREIREEEGEYISDMAKRLNISCEDIYAINRGKRELS
                     KNEINNVVKKYELEGEHLYLLKNITHKDKLFDIACNECGSKNVAIGMFDVANDIIEFR
                     CRNCNMIDVVSVDFY"
     gene            28817..29158
                     /locus_tag="KPK87_000044"
     CDS             28817..29158
                     /locus_tag="KPK87_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBG2536249.1"
                     /translation="MANIYCENYNCENYFEDMCMLERIEINNLKVCESYIEGKNELYK
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELIK
                     INSHLELEDLR"
     gene            29283..29726
                     /locus_tag="KPK87_000045"
     CDS             29283..29726
                     /locus_tag="KPK87_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2536250.1"
                     /translation="MKINFVIDGEPKAKARPRMSTANGKAYTPDQTVLYENWIRLMYN
                     STVKHFFEGNVKMTVICYFDITKKDREALKKNKVNTKAYKDAIGKVEGLIRPNKKPDL
                     DNIIKSVADSLNGIAYKDDSQIVEVVSKKYYSDRPRVEVELEDII"
     gene            29828..30682
                     /locus_tag="KPK87_000046"
     CDS             29828..30682
                     /locus_tag="KPK87_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBG2536251.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRELKHINELINNNIKRFKTNIDIIDLKVSRSERLGDLKANPSKGLGCEDVGYSKQS
                     FNQSRNVYLLSERGYSKLLKILEDDIAWEQYEKIVDGYFSMRKELNNPLLSASKELQA
                     IFMLDKKQEVLETKIENVNEKLENFMDDAPLFNIECECIVKEVKRVATKSLGGHGSKA
                     YKNKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNSYKLPIVFEEEIRLL
                     NSQLSIVS"
     gene            30825..31313
                     /locus_tag="KPK87_000047"
     CDS             30825..31313
                     /locus_tag="KPK87_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536252.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAIGYGEKVSPTYAFNSTVENEIIKKERDIARLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENELPLF
                     KN"
     gene            31895..32572
                     /locus_tag="KPK87_000048"
     CDS             31895..32572
                     /locus_tag="KPK87_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBG2536253.1"
                     /translation="MLKILQEKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     KTLKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            32635..33327
                     /locus_tag="KPK87_000049"
     CDS             32635..33327
                     /locus_tag="KPK87_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536254.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            33305..34558
                     /locus_tag="KPK87_000050"
     CDS             33305..34558
                     /locus_tag="KPK87_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG2536255.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYSKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANNKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     EAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            34573..36003
                     /locus_tag="KPK87_000051"
     CDS             34573..36003
                     /locus_tag="KPK87_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2536256.1"
                     /translation="MYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGEEYY
                     YGNADINNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDD
                     NLTKLLNDLLGEEFDDTITELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIPIWD
                     SKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMT
                     DIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLNFYKSLIDIYDNNISTLADNLDEI
                     QEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKN
                     IIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGSKSYDYKSVQITFNHSMIINESEKIDMAAKSIGIISDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            35993..37528
                     /locus_tag="KPK87_000052"
     CDS             35993..37528
                     /locus_tag="KPK87_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG2536257.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINNIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRHALVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            37530..37778
                     /locus_tag="KPK87_000053"
     CDS             37530..37778
                     /locus_tag="KPK87_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536258.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            37848..38411
                     /locus_tag="KPK87_000054"
     CDS             37848..38411
                     /locus_tag="KPK87_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2536259.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            38423..39322
                     /locus_tag="KPK87_000055"
     CDS             38423..39322
                     /locus_tag="KPK87_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG2536260.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            39322..39555
                     /locus_tag="KPK87_000056"
     CDS             39322..39555
                     /locus_tag="KPK87_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536261.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            39565..39966
                     /locus_tag="KPK87_000057"
     CDS             39565..39966
                     /locus_tag="KPK87_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536262.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
     gene            39960..40307
                     /locus_tag="KPK87_000058"
     CDS             39960..40307
                     /locus_tag="KPK87_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536263.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSCKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            40307..40732
                     /locus_tag="KPK87_000059"
     CDS             40307..40732
                     /locus_tag="KPK87_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG2536264.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            40725..41162
                     /locus_tag="KPK87_000060"
     CDS             40725..41162
                     /locus_tag="KPK87_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536265.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYLSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            41155..41331
                     /locus_tag="KPK87_000061"
     CDS             41155..41331
                     /locus_tag="KPK87_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536266.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            41332..42642
                     /locus_tag="KPK87_000062"
     CDS             41332..42642
                     /locus_tag="KPK87_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG2536267.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDKDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            42659..43129
                     /locus_tag="KPK87_000063"
     CDS             42659..43129
                     /locus_tag="KPK87_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2536268.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            43201..43641
                     /locus_tag="KPK87_000064"
     CDS             43201..43641
                     /locus_tag="KPK87_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2536269.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            43982..44143
                     /locus_tag="KPK87_000065"
     CDS             43982..44143
                     /locus_tag="KPK87_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536270.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     repeat_region   44881..45435
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=44881..44909
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            45569..46093
                     /locus_tag="KPK87_000066"
     CDS             45569..46093
                     /locus_tag="KPK87_000066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536271.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
     gene            46280..46462
                     /locus_tag="KPK87_000067"
     CDS             46280..46462
                     /locus_tag="KPK87_000067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBG2536272.1"
                     /translation="MSNKLGRPPKENSKKLRFEVRLNQEQADILNECAENLKISKTDV
                     VIRGIELVKEKIDKNK"
     gene            46580..47395
                     /locus_tag="KPK87_000068"
     CDS             46580..47395
                     /locus_tag="KPK87_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG2536273.1"
                     /translation="MMNELMNFEGKDIEVFEFEGQILFNPKHVAECLDISDVNSSIRK
                     FNDNQVVKLTNSKMHNMQFRKLHNTGENFLTESGVYKLIFKSKKEEAERFQDWISDEV
                     LPAIRQTGAYITNNADPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLLTA
                     KTLYKKAGIDLPIEINEEEHYFDTKQIASKLKLYSKSNKPAQMAVCEIIKKIDLEDSE
                     VKGVWETNGSWTGTVNKYTESVIDKIRNWIEENNRPTKIAGEKKNYHVVYKIE"
     gene            47447..47596
                     /locus_tag="KPK87_000069"
     CDS             47447..47596
                     /locus_tag="KPK87_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536274.1"
                     /translation="MHENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKREK"
     repeat_region   47673..48558
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=48200..48228
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            48689..49204
                     /locus_tag="KPK87_000070"
     CDS             48689..49204
                     /locus_tag="KPK87_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2536275.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREEE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     gene            49269..51662
                     /locus_tag="KPK87_000071"
     CDS             49269..51662
                     /locus_tag="KPK87_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG2536276.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVQGITTGTVLIIQGLGWITNAALNVANVFAQNWSIIAPAVYG
                     VVAAIAIYKGILLASAIVTGVSSFVNALYALGAYKACAALAAQELALFGKISAQTIEA
                     MVTAQATAAQYGLNAALYACPLVWIVLGFIAVIAVIFMVVAAINKFAGTSLTVLGVIV
                     GAVFAAVATIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DSVNNAGNALGKAFVDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNRWVGDAPEKVKLERMGYKDIGAEFQKGNALGTKWQNAISNKLKDTFDINK
                     MLEDAKDKLGLKDLWDKDNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            51678..52379
                     /locus_tag="KPK87_000072"
     CDS             51678..52379
                     /locus_tag="KPK87_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2536277.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWLICKKQLGNGSLYKKVYELNKSMMDKANKGKN
                     LSKYTIYKGQVLKLG"
     gene            52372..54264
                     /locus_tag="KPK87_000073"
     CDS             52372..54264
                     /locus_tag="KPK87_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2536278.1"
                     /translation="MADDLVLANDRDVRLIIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            54278..54538
                     /locus_tag="KPK87_000074"
     CDS             54278..54538
                     /locus_tag="KPK87_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG2536279.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESINPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            54543..54962
                     /locus_tag="KPK87_000075"
     CDS             54543..54962
                     /locus_tag="KPK87_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2536280.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            54963..56012
                     /locus_tag="KPK87_000076"
     CDS             54963..56012
                     /locus_tag="KPK87_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2536281.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TIDSNSIVVRGEVVG"
     gene            56005..56622
                     /locus_tag="KPK87_000077"
     CDS             56005..56622
                     /locus_tag="KPK87_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG2536282.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            56634..57626
                     /locus_tag="KPK87_000078"
     CDS             56634..57626
                     /locus_tag="KPK87_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2536283.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAENG
                     FKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSETRSELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIHEEINKVAEQLTGINSNIISTVNNDILPI"
     gene            57643..58761
                     /locus_tag="KPK87_000079"
                     /pseudo
     CDS             57643..58761
                     /locus_tag="KPK87_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            59098..59397
                     /locus_tag="KPK87_000080"
     CDS             59098..59397
                     /locus_tag="KPK87_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536284.1"
                     /translation="MKNIGRIVFYDNDTGDILMFYGEKTVSDSIDINIPKIDSIELPY
                     GSIDYTRNRLIGINVETKEPILEEIPVYVSEEEKRIQELENQILIAENEKVGGLL"
     gene            59397..59579
                     /locus_tag="KPK87_000081"
     CDS             59397..59579
                     /locus_tag="KPK87_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536285.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNREFQLDDVTNAEYRKAVED
                     YIIEHSGVVEGAEPTK"
     gene            59615..59845
                     /locus_tag="KPK87_000082"
     CDS             59615..59845
                     /locus_tag="KPK87_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG2536286.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLIGFFIFAIQRGIF"
     gene            59865..60122
                     /locus_tag="KPK87_000083"
     CDS             59865..60122
                     /locus_tag="KPK87_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG2536287.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLAL
                     GIGFSIWMLGLNADSVLQGVICWGVAIGINQTYKQLKEENK"
     gene            60122..60937
                     /locus_tag="KPK87_000084"
     CDS             60122..60937
                     /locus_tag="KPK87_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2536288.1"
                     /translation="MKIGVNCGHTKTGAGSGAIGKINESIETRNVGYKVIDKLKTLGN
                     NVVDCTIDKASTQSECLSKIATQANRQDLDWFISIHFNAGKGRGCEVYTYKGKQYQDA
                     IDVCKKISDLGFTNRGVKDGSGLYVVKKTKAKSMLIEVCFVDSEDANKYLSLGADKLA
                     TAIVEAITKHISSAEENNYNRYKHTIVYSGDDKVSADILGLYYKREKESYLVTDIKDY
                     KPHRTQNLYVIGGVTCNKMKELSKTTGEKFTEIYSDDVWSTMDKAREFVKGKL"
     gene            complement(61019..61201)
                     /locus_tag="KPK87_000085"
     CDS             complement(61019..61201)
                     /locus_tag="KPK87_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536289.1"
                     /translation="MSKDMIDECVHIITAYITSLKEYYSFIETQIDDFIKKYGEDIVE
                     YCLHRIMILLCECGLA"
BASE COUNT        24746 a         6791 c        11601 g        18707 t
ORIGIN      
        1 taataacttt aagatgatta atgatacttt tggatatgaa tttggtgaca atttattaat
       61 tacaatagca aaagctttaa aagaagaatt aacagaaggt gaggtatatg cacgactttc
      121 aagtgattat tttgcaattt tctgtgatta taaaaatgga agaaataaaa ttataagaaa
      181 acttgataat atacgaagta atattgaaag taatttgagt atagtctttg aaatttctct
      241 gtgtgtgggt atttattttg ttgaagaagg tgaagtagat attcaaaagg ctgtaaataa
      301 agcaaatatg gctaggtctg ttgctaaagg taaaaatata aattatgcta tttataatga
      361 agatgttagg aataagctta gtgaggaatc aatgatttta gatgatataa aaatagcatt
      421 ggtaaaaaat caatttgaag tgtattatca acctaagttt gcacttgtaa ctggagaaat
      481 gatagggagt gaagcactaa taagatggaa tcatccagaa catggtttta taagtcccgc
      541 agtatttatt ccaatagcag agaaaagtaa gttgatttta aaaattggga gatttgtatt
      601 tgaaagggtt tgcaatgatt tatatgaatg gaaaaagcaa ggtaaaaaga tagttccagt
      661 ttcagtaaat ttatctaggg tcgagttgta tcaaccagat attgtaaaat ttatcaataa
      721 aacaataaaa atgtataatt taagttcaga tttcattgaa atagagataa cagaaactgt
      781 ggctattaat gagttaaata ttttaaagaa tgttttaaat gagttgagga cgtatggatt
      841 ttctatttct atggatgact ttggtacagg atattcttct attagttgtt taagagatat
      901 gccaattgat atattgaagc tagataagtc ttttcttggt ggcattgaac atgatgagag
      961 aagtcgcaat atagcaaaat caatagtgtc tctagcaaaa tcactagatt tagttgtaat
     1021 tatagaaggt gttgagagta aagaacaagc tgagttaatg aaacaatttg gatgtgattt
     1081 agttcaagga ttttattttg cacgaccaat gccagctaag aattttttag atttacttta
     1141 aaacaaaatt tgatttttat ataaagcact attatgaatg cttgatttta ataaatcatt
     1201 gctaaataat tagtgttttt ttattttatc aatgtattgt ctaatataaa gcaaaaaata
     1261 attgtatttt ataaaaatta tattaaaaat aaatttattt tgctaataaa catgttatta
     1321 acaaaagaat agtactataa tgtatagatg ataaaaactt taataaaaat ttagtgtata
     1381 ggagttgttt aagtatgata gatataagta ttgatgacaa acttaaggaa agatggccta
     1441 atgctaagct gggttgcatt caagcaaaag tagcagtaaa taaaagctca gaaaaattaa
     1501 ttaatgaaat aaatgagttt tgtgatacta taaatcaaag tctaaaagtt gaagacatta
     1561 cgaaactaga taaaattaaa gatgcaagaa atgcttataa agagcttgga aaaaatccaa
     1621 gtagatatag aacatcttca gaagcattgg ttagaagaat tattcaaggg aagggtcttt
     1681 atacaataaa taatatagta gaaataaaca atttaatatc aataaaatca ttatatccag
     1741 taggtacata taatgtaagc aaattacatt caccagtatg ttttactgta ggagatgaag
     1801 gagaacagta taagggtatt gggaaagaat taataaatat agaaaatctt cctatattgt
     1861 ctgattcagt gggtaaattt gggagtccaa ctagtgattc tgaaagagca atgataacag
     1921 aagatgcaaa tgaaatatta atttgtatat tttcttttag tcaagaatct gatattgaag
     1981 aatattttgg atatgctaag gaattactta aagaatatgc agatggaaaa gatatagaaa
     2041 ctaaaataat taaatagaat ctatataaca acgttatgtt aagctaaaat tcaatataaa
     2101 aataagttaa gataaagaaa ctaacacaaa attagtttct tttttaaaat agtagtttag
     2161 tgttacaaaa atagatagat agtcccacga ttattaaaag tatggtaata tattaatata
     2221 gcagagattg tgtttaaggg attttgaaag gggggattta taataatcat tttatctaaa
     2281 agataaaatg gttttataga aataactata tatttaaaaa tataaataat gatacagtaa
     2341 tttgggtata taaaataagg catacatggt tttgtttaaa aaccaataag gtacataggt
     2401 aattacaatg taaggaagga tttaattatg attgatggaa aagaggtaaa acaagaaagt
     2461 ggacaaaaaa atctaaaatt taagatagct atagtagtag gtgttttgtt tgtagcttat
     2521 ataggaatgg caatcttttt tagaaatcat ttttactttg gaacgactat taatggcgta
     2581 aaggcttcag gaaaaacagt tgagcaagta aatgatttgt tagcatctag tactaattcc
     2641 tatactttaa acttagagga aagaaacaat aagaaagaac aaataaaagc aaaagatatt
     2701 gatttaaaat ttacaccaga tgataaagtt caaaagataa aagatagtca gagcgctatt
     2761 ggatggattg gtggaatatt taaatctaaa gagtataata atatgataac tctttcatat
     2821 aataaagatt tactagaaaa aaggtttaat gctttatctt gttttgatag taaaaatgtt
     2881 gtagaaccta agagtgctta tcctaagtat gatagcacta aaaacgctta tgttatagta
     2941 ccagaggtac ttggaaacaa agtgaaaaaa gatgattttt ataagttagt acaaaactca
     3001 attaattctg ggaatacttc tatttctctg gatgaaagta aagtttatga agcaccaaac
     3061 aacacaaagg attcagaaaa gttgaaacaa gctattaaga cattagataa gtatgttggt
     3121 gtagtcgtta catatgattt tagtgaccgt aaggaaactt tagatggttc tgaaattaat
     3181 aaatggttaa aagtagactc agaaaagaat tatgacataa cttttgataa tgatgctatg
     3241 gtagaatata caagaggttt aagtaaaaaa tacagtactt ttggtgactc aagaccattt
     3301 aaaactgcca caggtaaaga tattacaatt tcaggtggaa tttatggttg gttgatagat
     3361 aaaaagaaag aagctgaagc attagttgat gttgtaaaag ctggtaaaaa tgttacaaga
     3421 gaacctattt acatacaaaa agctgtatct cgtaataaag atgatattgg aaatacatat
     3481 gtagagatta gtctttctgg ccaacatatg tggttcttta aaaatggaga aatgcttgta
     3541 tcaacagacg ttgtaacagg tgatttagct agaggatttg ctacaccatc aggtatatat
     3601 cctcttaatt ataaggctag aaatgtttca ttaactggtc aaggttacag ttcacctgta
     3661 agttattggc ttccatttaa tgggaacata ggcattcatg atgctacttg gagaaattct
     3721 tttggtggaa gtatatataa gtcttcaggt tcacatggat gtgtaaatac acctttttct
     3781 aaggcaaaga aaatatatga aaatatagaa ccaggtacac caattatttt atactagtaa
     3841 atgtgttgct aacagttaag cgaagtcatc ttagtttatt taggatggct tcctttttat
     3901 ataaagctta ttgaagttta cagaaagact acttaaaagg agaaatacat aatgaatgaa
     3961 aaattaacta aaattttaga agaagctaag aatgatgatg aatttagaaa ggaacttata
     4021 aaaacaagag aacaacgtga tgttatggat tcattttgta aggttgttac atctagaggt
     4081 tatgatatga cagtagggga acttttttct atgggagaag aatatacatc caatctactg
     4141 aaaagtgtaa atggaggagc agcatatcct attgaagatt ggagtgattg gtacgaggat
     4201 ttttttataa tgttgtcgaa tatatagagt aactaatgat attatctact taagattaat
     4261 acattgcctt aaaataaaac tattagaact aggtgtaagt tttatcaatc tttaaaaata
     4321 ttgatttatt tctaactaga tataaaaaaa ttagattaaa aataaggtga caaaaatcga
     4381 tatagcttga tgatttataa aagttagata aaatagtgcg agatatttct aaaaattcaa
     4441 aaaaaacaat aaacttcaat ggttagaggt tgaaaaagaa tataaaaaac ttttatatgt
     4501 aataaattaa attaaaatta taaaaaaaat aaaataaaaa tttaaaatgt cgacataacg
     4561 ttaattttaa aacaaaataa cgctattgac aaactatatc gctttaaatt ataattattg
     4621 aaaataatgg tatttaaaaa ccatactcat gtcatagtct aaaagtaagt caaaaaagga
     4681 tgtaaatcta ggcaagtact attctaaaat atgttttatt aagaaataaa cgttgaaaaa
     4741 tattaattat tatataaaaa tgctgtttat actttaacaa tgaggtagta atctaattag
     4801 aataaaaaaa tctaattggt tatatacata gtaaaaaggt aagagaggtg atttggatgg
     4861 cttttgaaat aataaaaagc attgttgagg cagagcagac agcagacagt atcaaagtaa
     4921 aagctgttac tgatgcagag tctatcagag ctgatgctgt aaacaaatgt gaaagcatat
     4981 ttgctgatgt aaaaaaacaa gcaaagctta tggaagaaac tcttattgag aaggcagtca
     5041 ccgacagtag agcagaggtt gataaaatct tagctaatgc taaaagtgaa tgtctgaaaa
     5101 ttgaaaaaac tgctgaagaa agaaaaagta aggctattga agcggttatt ggaaaggtag
     5161 tgagataata tggcaatagt taatatgaac aaaatatcta ttgttggctt agaagctcaa
     5221 aaaagccaga ttttaaagct acttatgaac cgtgggtttg ttcagattga tgatagtgcc
     5281 tttcttactg aggaggagga attaaaaaat tatcttgtaa aagatagtga agaatctgaa
     5341 gttataatgc tagagcagaa aatgtctcat gtaaaccaag caattaatat tgtttcttca
     5401 ctagtgaaac aaaaaaaatc cctacttgca ccaaaaagag agtttaacaa aattgatagt
     5461 gaaaaagaag ggcaagttta tgctgaagct ctggagttaa acaatttgca caaggagata
     5521 ggtacaataa ggtctaacat aaacttactt aacaacaata aaagtatgtt agaaccatgg
     5581 gtgaactttg atattcctat tgaaaatatg gaaactaagt atacaaaaac tatacttgga
     5641 actgtgccca taagcgcaaa cattgatgct ttaagagtaa aattagaaga tgaaatacca
     5701 gaaagtgtta ttgggattgt caattctgac aagttaatga actatgttta tttaattact
     5761 ttaaaagaaa cttttgatga tgttttagaa gttttaaaag aatttaattt ttcagtggtt
     5821 tcattaccaa atgaagaagg tacaccgtta caagctatca agaaatacaa tgaaatgata
     5881 attagacagg aaaacgtttc taaggacaaa acagaggaaa ttaaaagaca tgcagatagt
     5941 atacacatgc ttgaaaacct atatgatttt tacactatag aaagagacca aaaaaagatt
     6001 atagagagac ttgtcaaaac aaaaacaaca ttctgtctta atggttggtt accaagcaaa
     6061 agggcagatg aattaattaa agaaattaca gaaaagtttg attgttgtgt tcagactgaa
     6121 gaaggaagta aagaagaagg cttcccaatt ctgcttgaaa acaataaatt agtaacacct
     6181 tttgaaagtg taactaacat gtacagctat ccaagtacaa aagatattga cccaaataca
     6241 attttaactt tcttctttgt tgtattcttt ggaatgatgc taagtgacgc cgcatatggg
     6301 ttaataattg cagtagtttg tggatttgtt gtatataaat taaagataca aaaaggtgaa
     6361 ggaaatttaa ttaaacttat tggtatctgt ggagtttcaa ctactgtatg gggtcttata
     6421 ttcgggggta ttttggggga tttaattcct ataaaagcat taattaatcc tttagaggat
     6481 gttatgacac ttatgggaat gtcgttactg ttcggtatta tccatatata tgtaggtttg
     6541 ggaataaaag catatacatt aataagaagt ggaaaggtaa tagatgcaat ctttgacata
     6601 ggtttttggt atttgtgtat aacaggtgta tgtctactta taataccttt tgttgcaggg
     6661 gacataggtg tattttcaga agtaggaaaa tatttagcaa ttataggtgc tataggtctt
     6721 gtacttactc aaggaagaag ctttaaaggt attcctgtta agctttttaa aggattttca
     6781 agtctctatg gtataacaag ttattttgct gatatattat cttatacaag aattatggcg
     6841 ctttgtttaa caacaggagt tatagcacaa gttatcaacc ttttaggagc tatagctggt
     6901 ccgatacttg cagttgtaat tggtgttgta ggacacacaa taaaccttct tataaatgca
     6961 ctaggtgcat atgttcatac aagtaggtta cagtatgtag aatttttcaa taagttttat
     7021 gaaggcggag gagttccttt tgttccgttt aagtataaaa caaaatatac aagcattaat
     7081 aaaaaggaga tgtaaatatg gaatttttaa acgcgttagg aaatggtcaa tttttggcaa
     7141 tatcaggagc tgctatagca gcattatttg caggtatggg ttctgctaaa ggtacttcaa
     7201 tagcaggtca agccgcagca ggtgttgtaa cagaagaccc tagtaaattc ggtcaacttc
     7261 tagttttaca acttcttcct ggtacacaag gtctgtatgg atttatcata gctttcctta
     7321 ttttatcaaa agcagggatt attggtggag atgcaatacc aacatcagct caaggtttac
     7381 aacttcttat ggcaggactt ccaattggtt tagttggttt agtatcaggt atagcacaag
     7441 gtaaagcagc agcagcagga gttggaatag ttgcaaaaag acctgaagaa cttggtaaag
     7501 ctataacatt tgcagcaata gttgaaacat atgctctttt aggactactt gtttctttct
     7561 tagcttacaa tggaattgca attggttaat tataaattat aattcataag taaaaaaatg
     7621 aaaaggagga agcactccta tgggtaatga acaaaaaatg attgacagaa ttatagctga
     7681 tgccaaacaa gaagcacaag aaatattgga taaagctaaa agtgaagcag atttaaaagt
     7741 aaattcagct aatgaaaaag ctgaaaagga aatggcttcc tatacaaagc ttgcagaggc
     7801 agaagcagaa aaagcggctt ctaaggaaat atcaggagct tatatggagg ctaaaaagca
     7861 aatattatca aaaaaacaag agattttaga agaggtaatt ttagaagcta aaaacaaact
     7921 tttaaatctt aaagataatg aatatgaaga gattatacta aacatgattg aaaaatcaaa
     7981 ttgtacagat gattcagaga tagtattatc taaaaaagat aaaaaaacat taaaagatgt
     8041 gctatcaaaa aaaggcatta aagtgtctga tgaaactaga gatattactg gaggtttcat
     8101 agttaaaaaa ggtgacattg agtacaatta ttcatttgaa gccataattg cagtagagca
     8161 tgaatatatt gaacaaattg cagcagaaat tttgtttaat taaggagggg ttaaaaaatg
     8221 gcagaagaaa aaacttatcc ctatgccgtt gcacgaataa gagtgcttga aaaacagctt
     8281 cttaacagac aaatgtttat tcaaatggca gaagccaaaa gccctgaaga ctctttaaga
     8341 attatagctg aggcaggtta tgctgataca tcaaataata atatacataa ttttgagaat
     8401 attttgactc aagaattatc aaagacatat gaaattctta aaagtctagc tcctgaagaa
     8461 aaatttgttg atgtatttct ttataaaaat gactatcaca atttaaaagt gctgattaaa
     8521 gaagaaattt ctggtgtaga tggtgagaaa tatttaattg atggaggtac aattcctctt
     8581 attaagctta gagaatcttt agcaaatcgt agttttagtg atttacctaa gataatgtca
     8641 tcagctatat tagaggcatt tgatgtttat aataagacac aaaatggcca gatggttgat
     8701 attgttctag ataaagcaac ctttaccagt atgaaagaaa ctgctaaaga aagccaaaac
     8761 aaatttgtaa tagattatgt tatgaaagtt tgtgatttaa caaatctaaa gagttttata
     8821 agagtcaaaa atatgaagaa agattttgat atgtttatga atgtgtttgt ttcaggtggc
     8881 tcacttgata aggaaaaatt cttagaagct ttttcctcag atactccagc atcatgtttt
     8941 aaatctactt cttactctga tgtctgtaaa aatggcatgg atagtggttt tactgtcttt
     9001 gaaaaacttt gtgatgacta tcttatggaa tatgttagag gagcaaaatt taagcctctt
     9061 acattagaac cactaattgc atatttatat gctaaagaat cagagataaa aacaattaga
     9121 attattttga ctagtaagct taataatata gacgcagata ctataaaaga aaggctaagg
     9181 gatgcttatg tataaggtag gagtagttgg agataaagat agcatcatgg gatttttagc
     9241 tcttggaata gatatcttcc cagcctatga tagtgacgaa attaaaaaaa gtatacataa
     9301 gttggttgaa gatgaatatg cgataattta tattacagaa caggcatcat tgcttgcaaa
     9361 agaatcaata gcaaaataca aagattacca acttcctgcc attatagtta ttccaggtat
     9421 tggaggaagt atgggattag gtatgaatga agtaagagaa tctagtaaac gtgctatagg
     9481 tgcagatata ttatttaaag aatagatgaa agttgaataa aagacaatct caaattttat
     9541 tggtgctaaa tatatgtact aataaaatat aggagggaat atttaaattt tgataaagta
     9601 tatgattgca ggtgagagaa tatgaaaaca ggcacaattg taaaggtgtc aggaccgctt
     9661 gttgtagcgg agggcatgag agatgcgaat atgtttgacg tcgtaagagt ttcagataaa
     9721 catcttatag gcgagattat agaaatgcat ggagataaag cttctataca ggtttatgag
     9781 gaaacttccg gtctaggacc aggggaagaa gtagtttctc ttggaatgcc tatgagtgtt
     9841 gaacttggac caggtcttat ttcaactatt tatgatggta ttcaacgtcc acttgaaaaa
     9901 atgtatgata tttcaggtac taatataact aaaggtgttg aagtatcttc acttgataga
     9961 acaactaagt gggaatttaa acctaaaaaa tctgttggtg ataaggttgt agcaggagat
    10021 ataattggtg gcgttcaaga aacagcagta gtagaatgta aaattatggt tccttatgga
    10081 gttaagggta ctataaaaga aatatattct ggtgaattta ctgttgaaga tacagtttgt
    10141 gttattacag atgaaaaagg taatgatata cctgtaacta tgatgcaaaa atggccagtt
    10201 agaaaagaaa gaccatatag agaaaagaaa acaccagatt cacttcttgt tacaggtcaa
    10261 agagttatag atacattttt cccaatcaca aaaggtgggg tagcagctat accgggacct
    10321 tttggaagtg gaaaaactgt tacacagcat cagcttgcta aatgggcaga tgcagatata
    10381 gttgtatata taggctgtgg cgagcgtgga aatgaaatga cagatgttct tcttgaattt
    10441 cctgaattaa aagacccaag aacaggcgag tcacttatgc aaagaactgt gcttatagca
    10501 aatacatcag atatgccggt tgctgcacgt gaagcttcta tatacactgg tattacaata
    10561 gctgaatatt ttagagatat gggatatagt gttgcactta tggcagactc tacatcaaga
    10621 tgggctgagg ctcttagaga gatgagtggt cgtttagagg agatgcctgg tgaagaaggt
    10681 tatcctgcat acttaggttc acgtcttgct caattctatg agagagcagg aaaggtaaat
    10741 tgtctaggta tggatgaaag agaaggaaca cttacagcaa ttggtgcagt ttctcctcct
    10801 ggtggtgata catcagaacc tgtcagccaa gcaacgcttc gtatagtaaa agtattctgg
    10861 ggtcttgatg catctttagc atataaacgt catttcccag caattaactg gttgactagt
    10921 tattcactct atcaggaaaa agttgatgta tgggcagata aaaatgtaaa tgataatttc
    10981 tcagctcaaa gagctcaagc tatgaaactt ttacaagttg aatctgaact tcaagaaata
    11041 gttcaattgg ttggggttga ttcactttca gatggagata gattgatact agaggttact
    11101 cgttctataa gagaagactt ccttcatcaa aactctttcc atgaagttga tacttataca
    11161 tcacttcata aacaatatag aatgatggga cttatattaa aattctataa gtcagcacaa
    11221 gatgctatca agcaaaatgt tactataaac gatattatca agctttcagt tcttgaaaag
    11281 attggtcgtg ctaagtatgt agaagaaggc gatatagatg cagcttacga ttcaattgaa
    11341 gatgaaatag acaacgagct tgcagacctt attaaaaaga ggggggctga agaactatga
    11401 taaaggaata taagtcaata caagaggtag ccggtcctct tatgttaata aacggcgtag
    11461 aaggtgtaaa atttgatgaa cttggagaaa ttgaactttc aaatggagaa attcgccgtt
    11521 gcaaagttct agaggtaaat ggagatactg cacttgttca gcttttcgaa agctcaacag
    11581 gtataaatct tgctgaaagt aaagttcgtt ttttaggaaa gagtcttgac tttggagttt
    11641 caccagatat attaggtcgt gtttttagtg gaatgggtag acctattgat ggaggtccag
    11701 aaattatacc agataaaaga cttgatataa atggagctcc aataaatcca gcagcacgtg
    11761 attatccggc cgagtttata caaactggag tctctgctat agatggactt aatacattgg
    11821 ttagagggca aaaacttcct atattctcag ggtctggttt gccacatgcc aatcttgcag
    11881 ttcagattgc aagacaggca aaagtacgtg gaactgattc taagttcgcc gttgtatttg
    11941 ctgcagttgg tataactttt gaagatgctg aattttttat aagcgatttt aaagcaactg
    12001 gtgcaatcga ccgttctgta gtgtttgtaa accttgcaaa tgacccagct gtagagcgtg
    12061 tatctacacc tagaatggca cttacagcag cagaatacct tgctttcgag caagatatgc
    12121 atgtacttgt tataataacg gatattacaa actatgcaga ggcattgcgt gaagtttcag
    12181 cagctaaaaa agaagttcca ggacgtcgtg gatatccagg atatctttat actgaccttg
    12241 ctactatgta tgaaagagca ggaaaaatga aagggcatga agggtctata actatgattc
    12301 caatacttac aatgcctgag gatgataaga cacatccaat tcctgacctt acaggataca
    12361 ttacagaagg gcaaattata cttagccgtg aactttataa aaaagatata cagcctccta
    12421 tagatgtttt accatcactt tctcgtctaa aagataaagg tattggtaaa ggtaagactc
    12481 gtgaagacca tgcagataca atgaaccagc tttttgcagc ttattcaaga ggtaaggatg
    12541 caaaagaact tatggctata cttggtgaat ctgcactttc tgagattgat ttaatgtatg
    12601 ctaagtttgc agatgaattt gagaaagaat atgtttcaca aggatttaga actgatagaa
    12661 ctattgagca gacattagaa atcggatgga agcttcttag tatgttacca aaaagtgaac
    12721 ttaaacgtat ccgtgatgaa tattttgaaa aatatatgcc taaggagtga tatctgtggc
    12781 tagattaaat ataaatccaa cacgtatgga aatgactaga cttaagaaac tccttaaaac
    12841 tgctacaaga ggtcataagc ttcttaaaga taaacttgat gaacttatga aacaatttct
    12901 tgagattgta agagaaaata agcgattgcg tgaagaagca gaaaatgctc ttgatactgc
    12961 ttataaaaac tttataatcg caagagcagt tatgagtcag gagtatctgg gttcagcact
    13021 tatgatgcca aagcaatcag tttcagtaga tgtttctacg agaaatataa tgagtgttga
    13081 tgttcctgtg tttgacttta aaactgaaaa caatcaaagt gatatttatc cttatggtct
    13141 tgcttttaca tcaggtgaat tagattctgc tatggaagct ttctcagatg ctatgcaacc
    13201 acttttaaga cttgcagaaa gtgaaaaaag tgcacagctt cttgcacaag agatagaaaa
    13261 gacacgtaga cgtgttaatg ctcttgaaaa tgtaatgata ccaaattaca ttgagactat
    13321 taagtatata gctatgaagc ttgaagaaaa tgagagagca agtactacta gacttatgaa
    13381 ggttaaggat atggtgctta aaaaagcact tgaagaaaag aaaaaaaatg atttggttgt
    13441 ataattaaat acttataaaa ataagtctaa cattctactt tatagaggtt agacttattt
    13501 tttatgttta aattaaaatg tagatactat aaaaattaat atattgttta aaaacagact
    13561 gtatttaact aaaaataata tatttaacaa aaatttctaa aaattaacga actgttaatt
    13621 tacacttaac tttatattaa caaattaggt gtattatata aacataagca aaaacaataa
    13681 aaaacaaata aaaaaaggga aaaacacaaa ggggggaaga tatgaaagaa tttatattta
    13741 cttgtctgtg cattgtagtg attacattta tgacaagttg catatcaaca acaccacaaa
    13801 ctactcaagg aaaaggaaat aataaactta caatatatgc agcactagag gaagagcaga
    13861 tagcttatta tatggaaggt tttagagaag agtatcccaa tatagatgta gagatagtta
    13921 tggattctca tggtgtaata gcttcaaaac tattagcaga aaaagataat ccacaagcag
    13981 atattatatg gggattatca gcaatgaaca tgatagagct aaaaaaagat ggtgcacttg
    14041 aagcatataa gcctaaaaat tatgataaag ttgataaaaa atttaaagat actgaagatg
    14101 atgtatcttg ggtaggaatg agtgtcccag agacagcaat agtagttaat tataaggaac
    14161 ttaagaaact aggtatagat attccaaaaa gttatgaaga tttaataaaa ccagaatata
    14221 aaggacttat aacaatgcca aatccagcat catctggaac ggggtatctt acagtatcag
    14281 ggcttattca aatgatgggt gaaaaagatg cttacaatta catggataaa ttacataaaa
    14341 atatagccac atatacacac tcgggttcaa aacctgcgaa gttagctggc tctggagagt
    14401 atccaatagg tattacactt ggatatagag ctacacaaca attatctagt ggagaaccta
    14461 tagaggttgt atttccaaag gaaggttcag gttgggactt agaagcaaat gccttagtta
    14521 aaaaagataa tataaaaaaa gaagcaaagc tatttttaga ttgggcaata agtgattcag
    14581 ctatggatag atatgcacat gaagtagcag ttacttccat aaagacagat aatccagttc
    14641 caaaaggtta ttctaaacaa ccatttgaac agcttataaa agaagttgat ttaaactctg
    14701 ctgccaaaaa cagaagtaca gtacttaaaa cttgggaatc aaaatacggt tcaaaatctg
    14761 aaagtaagta aagtttaaat taataattta gatgaaagaa ttttagaaga aaaagtagga
    14821 tataaaattt aaaataaaaa aataactata taaggagaga taagatatga gttacttaaa
    14881 aataaataat gtgtttaaaa gttatgacca aaaaaaggtt ttaaataata ttagtttgga
    14941 tattgaagaa ggggaatttc tatgtttact cggacctagt ggatactata tgttagtcca
    15001 aataattata taaaattaat agtgatgttt ttgttattat ctatttctat ataatctatt
    15061 atagataacc aaaaacgtct gcgttcaacc ctgcttaaat cactatacat ctcattaata
    15121 tcaatatcta agaatttttt tatatgtgat gtatcaataa tttgactctc ctgttcttca
    15181 gttatattat ccagttgaga ttgtaatttt tcataatctt ttctataagt ctctttatca
    15241 atcaaatcct ctaaatataa atctttgatt ttatctaatt ttttcatgat tttcttcttt
    15301 tcatcttttt tgttagattt cttatttcgt ctgatttttt cattttgtgc ttctaatttc
    15361 accatatgtt tatgtaattg tggttttaag ttagtcaaaa ggtagttttc tatatgtttt
    15421 tcattatgag ttacattgtg tacacagttt ttatttagat agaatgtaga acatctataa
    15481 gataaataaa gtttttctcc aacattacta gaattaccct ttaatgtata accacactcc
    15541 ttacatttta aaagaccaga aaaaatatga taatgtatgt ttttattagg agtagttttt
    15601 ttattttttt ctaatattct ttggatttgt ttaaattgtt tttttgatat aataggttca
    15661 caataatctt caacataaaa tcctcgttta ttatagattc ctatataaag ttcatttctt
    15721 agtatattct ttattttatt ataactgtat aattcgccat gaagattatt aattgtttct
    15781 attaaagtcc taacagaacc tgatttctcg tattcgtcaa atatagcttt aactatatat
    15841 attttttctt tatcaataac tagatgctta ttttctattt tgtaacctat gggaattgta
    15901 ccacttataa cttctttacg tctcaattta tcctgaaaaa caaattttat tctttcagat
    15961 gtttgagctg cttcattttc tgctacagat agcataatgt ttatatgtaa tctaccagca
    16021 gcagtgctag aatcgtagtt ttctaaaatg gttttccaat cacacttatg tttttccaag
    16081 gtttccatta ttttataata gtttttaacg ccacgactta gtctatcaat tttagtcatt
    16141 aaaactaggt ctactttatc tttttccaca tctcttaata atcgttgtaa gttaggtctt
    16201 tttaaattag ttgcagagca accttcatca atgtatttac caactatgtt aaaatcattc
    16261 tctttagagt actgttctaa agcctctatt tgtgtcctta tgctatctcc atttaaagct
    16321 tgttcttctg tactaacacg tatataaagt gctactctta acattttaca ttccccctta
    16381 ttgtatattt aagaggagct gaacggctct taaatatact atgaattgtt acaaaaaatt
    16441 tgcattcata atactaaatc ttggctcaaa aaatatcaca taattatcta gtgtagctga
    16501 aaaaccatat ttacttttat aacattctaa agcttcattt agaaactttt cagttacatc
    16561 aaggtattct gccatttcgg ttatagtctt acagccagca ttaaaactat ctattatacc
    16621 tttaagacca attaacttgt tgtaagccat caatctagct ttatattctt gtttgctatt
    16681 ttcaacttta tttaggtcta atatatttcc atatgaagta taatgatgtc ctagttcttc
    16741 tgctaacaca caagcttttt cacttatatt attaagtgta tttttattta aagctattct
    16801 attattttta tataatccat gagaatttga aattaaagga acttctctaa caataatatt
    16861 attatcatct gcttcagaga gtaacttttc ataactattc ataagttcac ctacctattt
    16921 taccaattat ccatatcatc taaatcttga tatatttttt caatttctcc ttcttcatgt
    16981 aaatgttcat tgtgtgccgc tattgtgtct atgtgatttt tatttacata tttacctatt
    17041 tgagtaagtt catctactct ttttattgct tcattttgtc caatatcgtt taaggcatta
    17101 aagcttgata aaagcttatt ttctttaatt ttaatttgtt tgcagtcttc gttccacccc
    17161 attataaaag caggagattc atctaatgca tgtgctaaaa tttctagctt atcaagagga
    17221 atatttttta tagctcccgt ttcatatctc tgtaaagtgg acttacttag tccagtcttg
    17281 ttagctaaat cttgataaga catattaagt tctaatcgtt tattttttat tctatttgta
    17341 atctccaaca tttttaaatc attttcagta ttcaaattta tcacctctat aaaatatatt
    17401 ataaagtatt ttttcataaa tgcaacacat atattggaaa atataaaaaa atgttgcatt
    17461 tttgacttga caatataaaa tggatgtatt aatattgaat tatcccaaat aagcaacgag
    17521 gtgatggaat gaatataagt aaattaaaag gaaaaatggt cgaaaagaaa tacactcaaa
    17581 agaaattagc agaagagttg ggtatgactg ttcaagcttt aaatgcaaaa ttaaataatc
    17641 gttcacaatt caagattgaa gaagcagtta agatatcaaa tattttgcaa atcgaagagc
    17701 ctggtgagat tttttttgac gattatatcc caaaaatgca acgaaattaa ataagaaaac
    17761 ctaacagata aaaattaaaa ctagggaggt agaaattatg agtaataatt tacaagtaat
    17821 agaaagaaac aacaaaagag ttctaactac acagcaacta gcagatgtat atgaaactga
    17881 ctcaaagaat ataagtaaca atttcaacaa taacaaagat aggtttattg aaggtaaaca
    17941 ttatttttta ttacaaggtg atgatttaaa gaattttaaa ggtattcata cggaatatca
    18001 agtgatttaa gtaagaatta agttaaataa aacaagtacc ttgaaaacta aatacagaat
    18061 attcaaaaga ggtgattaga tgcaaataga gcaaacaaca atacgcctgc ctatataagg
    18121 cgaataagcc caaaagagga cttattcttt tgtttctttg catttttcag catttaattt
    18181 ttctaaacct aatttgatta attcaatagt agcttgtgtt cttgtagaga accttttatc
    18241 aaacctaaaa tcatcaattt cttttaaaag ttcatcatca acagttattg tgaaccttgg
    18301 tttttgagta gccattacat cacctcacct taagtgtatt atacaccagt ggtgaaatgg
    18361 tgtaaatata aaaaataaaa atataataaa aaagtgttga catggttcac cagttcatct
    18421 ataataaaaa tataaagagg ttcaccactg gtgacaaaag gaggatgaaa atgccaacta
    18481 aatcaaaaag aatttcaata actatatttc cagaattaga aacagattta gatgttttaa
    18541 aaaaggaaaa gttttataaa gaatctcaaa gtgaaatgct aaggtatctt attaaacttg
    18601 ggcttcaagt aaataaagaa aaggtctata aaaacgaata aaagaggagg tataagcatg
    18661 aatgacctaa caataatcaa gcaaaataat caattcttag ttgagagtag agaagtcgca
    18721 gagttaatag aaaagaaaca tgataatttg ttaagagata taagaggata caagaaaatt
    18781 ttagaggact catcaaattt gaggagtcag gattttttca tagaaagtac ttatataaat
    18841 actcaaaata aaattcagcc ttgttactta ttaactaaaa aaggttgtga catggtagct
    18901 aataaaatga caggtgaaaa gggaattata tttacagcga tttatgtaac taagtttgaa
    18961 gaaatggagc gagagttaaa agaacaacaa cctaaactac caactacata caaagaagca
    19021 ttgcaacagt tattaataga agttgaagaa aaagaacaat tacaattaga aaatcaagaa
    19081 aaagataagg taattcagtt acagcaacca aaagtactgt ttgctgattc agtagcatct
    19141 tctgacaatt caatcctagt tggagaatta gcaaaattgc ttagacaaaa tggaattgat
    19201 acaggacaaa atagattatt tgactggtta agaaataatg gttacttaat aaaacgtaaa
    19261 ggcgaggatt acaatacacc aactcaaaaa agtgtagatt taggagttat agaaacaaaa
    19321 gaaggtacaa gagtacatcc agatggtcat acaagtatta ctaaaacacc taagattact
    19381 ggtaagggac aaatatactt tattaataag tttaaaaaga acaatcaaat atcaatgtta
    19441 agctaaatca taacagcact ttgaaaacta aatacggaat attttgaaaa tagaggggtg
    19501 atttaaatgg gtaaaaaaag tgaatacaca gtaacaacag ttggtgaacc aaatttagat
    19561 gcattaggcg aaactttagc taaaattctt acagagaaat ttggggtact aataactcat
    19621 acatacattg agaactatta tgttgataaa gagagagggg gtgatgtaga tggagctatt
    19681 gaaagtgaac tttaatctag agaaattaaa aaataggaaa tgtagtgagt gtaagttcta
    19741 agaggtttgg gttcaatatt aattgtattg tagtagtagc aactgaggag gaactaaatt
    19801 tagcttatta tagcaaagat aagggatgta ttgagtatca agcattgata ccagaggata
    19861 ttagatatgg tgattatatt cttgaaagat taggttagga ggaaataaaa tggcagcttt
    19921 aataatagta ggtttatttg caatatgttt agtaggatta gttcaaaata gagattaatt
    19981 aaaaaggggg tttaattatg gaaagtttaa aaatagtaaa acaatatgtt gaaggacagt
    20041 taaatttatc tagtttagaa atagacaaaa ataaggagac ttatgagatt ctcaaaaata
    20101 aatctagcag agatatgtta gatgacatta atttaaatga tgcattaaga gaagtaacag
    20161 taaacgaaag attaaaaata tttgctgagt cattactaga gttgttagat acacaaatca
    20221 aaataaaaga gagtgaagaa agtgaggact ataaaagact ttgtatgtac ttagacgaat
    20281 ttggaagaga tagacctata gatgtccaga tataaaaaaa gagccattag gagtggctcg
    20341 attcagaaaa tatttaaaaa taaaaatatg actatattat agcataaacg gagggaaatt
    20401 atgagtactt tatatgaatt aactacagat ttattagaaa tagaagaagg tttaacagaa
    20461 ataacaggaa atgaagctga aaaactagag gaaataaaag aaataataaa acaagagata
    20521 caaaataaaa acactaggat agtttcagtg ataatagata ttgatagtga tataaactct
    20581 ttagacttag agattaagag aatgcaagag ttaaagaaga ttaagaagaa tagtcttgat
    20641 agattaaaaa gtaacataaa ggaatgtatg gaactgcttg gtcttaaaaa ggtagaaaca
    20701 tttttaggaa atataagtat aagaaagtca gcaggtagct tagtcataga agatgaagaa
    20761 aagatacctg ctatatataa aacagtagag caagttgtaa aagtagataa aaataccatt
    20821 aaagacttta tcaaaaaagg tcatgaggtt gaaggttgta ggattgaata tggaactaca
    20881 ctaacaattc caaaagctaa aaaagagtag gtgaggacca tggaaactaa taatgtttat
    20941 ataaaacttg taaatataca gagtacttta aaagctccta aaagtcaatt taatagcttt
    21001 ggtaaataca actataggag ttgcgaggat atactagaag gtttgaagcc tattctaaaa
    21061 gaagaaaaag cattagttat attagatgat aaagttgttc agataggaac tagattctat
    21121 gtagaagcta cagcaacttt aatagatgca gaaacaggag aaaaagtatc tgcaaaagca
    21181 ttagcgagag aagatgaaac taaaaaaggc atggacttag cacaagtgac tggaagtgta
    21241 tcaagttatg caagaaaata tgctttaaat ggattattct gcattgatga tacaaaggat
    21301 agtgatgcaa caaatacaca tggaaaagaa caaaaaaaaa gagaagttag tgaagatgaa
    21361 ttaagtacac tatatttttt aggtgagtct atagataagg ataagaatag agtcgatagt
    21421 gaggtttata agaagtttag taaactagca atagatttga ctaagcaaga gtatgaaaag
    21481 gttctaaatg gatacaagag cattttagat aagcaaaatc aagagtaggt gatatattga
    21541 attttaataa tgaaacatac tttcacataa attttgacga cccatttaca agagtaccta
    21601 atacaatcct tgataatgaa aatcttagtt attcagctat aggagtagtt actcaaatgt
    21661 taaggtttca aaggtcgggt agtcataaag tttatgcaaa atcattaata agctatagaa
    21721 aagatagtaa gacaaaagta agtaatgctt taaaggagct tatgcaggaa ggttttgtta
    21781 ttaggacaca aataagggat gaaaaaggtc aaatgaaagg ttatagatat gatatttttg
    21841 atacacctca aaatgtaaat tctgaaagtg ttgaaacgac tgaatctcaa ccgtgtgccg
    21901 ttttcccgac tccggtaaaa ccggaagctg gtaaaaccga agtcggtgaa accggaagcc
    21961 ggcaaaaccg aggtcgggaa atcggcaaca taaaagaaaa tagtattaaa aagaaaatag
    22021 gtttaaaaga aaatgatgtt attactactg ttattgctga acaatctgaa aaaaataaga
    22081 ctgtttacat aaaaaaatat tatgaatctt atataggtgt gattactcca aataattttc
    22141 ttcaactact gacttattta gatgatggaa tggaagctga tgtaataata agagctgttg
    22201 atgaagctgt aggcagtgga gttaagaatt ataagtatgt aaaaacaatc ttaaataatt
    22261 ggatagaagc aggtgtaaaa actgttttag aacttacaga atatcagaat gaatttgaaa
    22321 gaaagaaaaa gagtaagcag gaaaagaagc agtctaatag taaaactgtg aatactcata
    22381 atgtgaataa aaataagttt gctaacttca atcagacttt cactcaatat gaagaaaaag
    22441 agctagatga gattattaaa aagagtcaga aggaaaaatt taaataaaat taaacttcta
    22501 ggaagtaaat atcaatatat tgcttcctag aaaaggggag gtataaaatg gcgagaatat
    22561 atgcacaaag aagtggttct ttaaatgaac aagatagatt ggagttatta agattacttg
    22621 ggaaagctgg atatacagta aaggttgcta gagagaagca aaatagcaag acaacttata
    22681 cttactttgt tgagtataca gaagaacagg aagaaaaata gaagggggct agttaaatga
    22741 atacaataac tttagttgga agattagttg cagatgcaga attgaagtac cttccaaatt
    22801 caggtactcc aaaaataacc ttttcaatgg cagtagatag aaggtttaaa gataaaaatg
    22861 gaaataaaat aactgatttt attcaatgcg agcaattagg aaaacatgta gagaatttag
    22921 tgcaatatct tgttaaaggt aagcctatat atgctgttgg agagttaaat atatataatt
    22981 acaaagatga aaatggttgc tggaaatcta ttactaaagt taacgtgaat gctttagaat
    23041 tactttctag taaaagtgat agtaataatc ataaagagca acaggaatat ataccaccag
    23101 gactggaccc acaaggtttt caagcaatag atgatgacga tatacctttt taattaagtt
    23161 aagtagtcta gggagtaatt atacaatatt acttcctaga agttaaaaaa tattggaggt
    23221 ctaagagtga aatatgagtg tgagaaagtg ttcttagaat gcgataaggg aagttttgag
    23281 ataaatgata caagaattgg agaagtaatg ttcgagagta cagaaataga caatccgttt
    23341 gagagagtaa aatatgaagg gacttttgaa atagtatctg gatgggagta tctacaaaga
    23401 gaaatgttgt ggcttaagat attgcattta tcagcaattg tagcaaaaat aatgcaatat
    23461 aaaatgttag gtatttcaaa atgaggaagg ctgaaatatt ggaaaagaca agaaaaaata
    23521 agattagtgt ttgttatcaa tgtagaaatg aagatataag cgaagatgct agatattgta
    23581 agatttgtgg aataggatta agagttatgg aattagtaag tgtttttaac tttgaaacag
    23641 gagaaaaaga actttcattt ttaaaaggta aagaatgtct tatagatttt ggagatttaa
    23701 gagaaggcaa tatgtgtaaa ttattctttg aaaatacaga attaagaatt attggagtag
    23761 aaaatattta tcaagataaa agaggtatat ttattgaagc tggtgagtgc agccatgaaa
    23821 taatatttaa agatttggag tggtagaata tggctaaaat ttgggtagat gcaggaacgt
    23881 ttttagaaaa aactatggat ttagaagata tgtttgagct taatttaaga aaagtaagaa
    23941 aagcaaatga aagtaagaaa ataaagctga aacttaatga atcgaaattc aagaaaataa
    24001 ggaaaaaaac aactaatgat acaaaaagta aacctataaa agtttttaat attgaaactg
    24061 gagaagttag aatatttaaa agtgcaaagg ctgcaagtaa atacttaaag attagtggag
    24121 attatgctag ttgtttagct agagaaaata gagtaactag agaaggttgg aaggcggaat
    24181 atattcaaga ggtgtcagat ggtattagca aatgtggaac aagtaattaa gttagctgaa
    24241 aagatattaa ataagaaaaa gtgttctgtt aataaagcta ttgatatagc tataaaaata
    24301 ttgagtagat atgagtatga ggggatgttg agaaatgaaa gtacaaatgc agttaactaa
    24361 agacaaggag tttttcaaag tttatgtaaa ttcagaagaa gaagagctgg agagattgtt
    24421 ttttgaattt gtatcacaaa tgttagctta taaaagaaaa aataagaagg ttcaaggaga
    24481 tattgagaaa tgaaattaaa agatattata aaacttggag aaaagtattg ttattgccct
    24541 aactgtggta atgacaaggt aggaaacaat gaaggtaaat taatagttga agaacacaca
    24601 tattatagag aatgttcatg tgggtttaat atactgattg atgataggaa ggatgaaatc
    24661 taatgaacat gttagctagt gtgatattag taataggaag ttttatagct ggtagagttt
    24721 atgagtatag attgaattta aatgaaaatg atgaagctga ttcaaaagta cttttagatg
    24781 tttttaatga aattagtgtg ttaagagaag aaaataaaag tcttaaagaa aagttacaag
    24841 agaaagagtt actattcatc aatagattaa tagatttttt acatgataaa aagatatgcg
    24901 aatgttgtat ttatgactgt aagattgatg atattgaata tgattgtgaa gatggtatta
    24961 aaaagtggct tgatagcgaa gaacttatat ttgaatagaa gaaatatcta attaaaacag
    25021 tttagagagt tgcaaaatgt tttttaataa aattatcatt gagatgtttt gtaactctca
    25081 aaaatgaaaa taaggaggcg ttgtattgct tacattttta gatttattcg cagggatagg
    25141 tggctttagg ctagggatgg aaaaagcagg acataaatgt ttgggacatt gcgaatatga
    25201 taaattcgca aatttaagtt ataatgccat gcacaaaccg aaggaggatg aatggtttga
    25261 aagagatatt agagaaatta gaacagaaaa tatcccaaga gcagatgtct ggtgttttgg
    25321 attcccatgt caagacattt ctgttgcagg gaaacaattt ggattcagag gagaacgttc
    25381 aagtttattt tttacagtta caaaacttat tagagaactc aaagaagaag atagacccaa
    25441 gtatttactt attgaaaacg ttaaaaatct acttagtgtt aatggaggat ttgatttcct
    25501 caaagttctc gttgaactgg atgaaatcgg ctatgatgca gagtggcaag ttcttaattc
    25561 taaaaacttc ggagtgcccc aaaatagaga acgaatattc attgttggac attttagagg
    25621 acgaagtaca cgaaaagtat ttcctatcga aagaaaaagt ggaaaaaatc ttgagcaact
    25681 aaataatcca actcatagta caaatagaat ttatgatgca gttggaattg ctagatgtat
    25741 tagaagtcag gcaggaggtg gaggtgctaa aacaggtcta tactttatag acttaaataa
    25801 aaactctaaa gtaacaataa atgctagatg ccttaaagca aaatataatg caggtgtgac
    25861 aaatagaaat tgtgataata gtggagtttt agttaatgca gttttaacgc ccgatagggt
    25921 aaataaaaga caaaatggtc gtagaattaa agaaagcgga gaaccaatgt tcacattgac
    25981 agctcaagat aaacatggaa ttttgaaaaa tggagatata agaaggttaa caccaaagga
    26041 atgctttagg ttgcaaggat ttccggataa atattacgaa agagcagcaa gtgtatgctc
    26101 agatagtcaa ctgtacaagc aagcaggaaa tgctgttact gcaaatgttg tatatgaaat
    26161 agcaaaaaga atgggctaaa agttgcaaaa tgtcttttag tatgaatatt tttgaagtgt
    26221 tttgtaactc tcaaaaatga aaataagggg tgggataata aaatggattt aaataaaatt
    26281 atgaatgatg cactagtgga aatagaagaa agtggctttg tagaggaaac agttaaaaaa
    26341 gaattagaag aaacaataaa aagagctgtg aatgaaatct ttggaagcta tagtaaattc
    26401 agaaaaaata ttgaacaaca tttaggtgaa aatataaata taaatttaga tgaattagat
    26461 atacaaaaat acaatttact tgtggcaaat gttgtaaaag aaaaggtaga tgctacgatg
    26521 aaggggcaag gtattgagca tttaaaaaga aacttggata acatgcttgt aggtcttaag
    26581 aaagaataca aaatgagtga attattagaa gaattgaaag aagataaata caaccttgac
    26641 gagtacccat gtgatgaaga ctgtataaca tttatccttg aatctaattt taattcagaa
    26701 tgggcaagat ggatagactt tgatgagtgt cctaatgtaa gcagaaataa ttgtgaacat
    26761 tctatactat taagaaacga tggaactatt gcagcattta gataccaaaa cagagaaata
    26821 tcatcaaaag atataatgaa cggatttggt aattttgggg atttattatt taagatatat
    26881 gcacataatt caaaaattat cttagattta gggaatgatg tagacgatta tgacttaaca
    26941 aatggagaag attattagaa taggggtgaa taaatgtcaa agtatgtatt gagatggcaa
    27001 aaaggattat tactagatga acgcaagata aattattctt gtggaagtat agaaaattta
    27061 aaaaagaaag ctgaattatt agctaaagat gacaagatat tgcttgtaac aatagataaa
    27121 gttgaagaag ttataaaaga tactagaagt caaaaaatgg ctgaatatta ttgtgatgga
    27181 ggaattgaaa tatgataata cacaaattta taatacatgt tttagataag aatagcgata
    27241 caccaatact aaatgatttt gagggtagag tcagtcaaga tattgaagct ttctttcaga
    27301 aaaaaataag caaagtatca agagataatg acattagaat agctgtattt aatgactata
    27361 gtaacaatct aattaagagt tgttgcgaac agattatata tgatgaaagt tcatttttaa
    27421 ataactctaa agaaatagca tcttatctgt ttgagattat gaagttgaat gctacattag
    27481 aatcttgcga cttagcaatt tgcttatact ctcaaaaaga tgaaaagaaa gttgctatat
    27541 taaagcttga ttacaacaag tcatatactc attcaatcga gtttaaagat gataaattta
    27601 acatacaaat gtctaaaaat gaaattaata tacaagagac taagacagta aaaattgctg
    27661 ctttggttgg tttgagtgga atgaatgacg aatatcatct tagggtttta gacaagaatg
    27721 cagaaaagga agaagctaat tctaagtttg ttacagagtt tctaaatgct actaaagtga
    27781 aagatgacaa gtacaagact aagatgttta aaagtacagc cgaaaattgg ataactaatg
    27841 ctcttggaaa tgatataaaa caggcagaag atgtaagaag tatattaaat tatactttga
    27901 aagaaaagca tgaaattgat ataaataatt ttgttgataa aacaattaaa gatgataagt
    27961 taaaagatag ctttaaagaa catatggaag aaaaaggtct tgataaagga tttagtatag
    28021 ataaaaaatg ggttgagaaa aagcttaaaa agagaaatat aaaaactgac aatggctttg
    28081 atataaaagg taatctgact gtttttgagg acccaatgaa atatacagta agacaaaatc
    28141 aaaatgggtc tatagatata attattaaga atgtaaaatt ttacgaagaa aagtaggtga
    28201 gcatatgact aataaagaaa tgtgcaagtt aaaaaatctt gatgaaagag aagtgtataa
    28261 ggaatttggg aaagagattt gtggtagttg caagaataat aagggagatt gtgaaagtaa
    28321 aaattgtagt gaagcatgta aaaattggct agagaaggtg tgtaaatctt gactgaacta
    28381 ggagagcttc ttagagaaat aagagaagaa gaaggcgaat acatatctga tatggctaaa
    28441 agattaaata ttagttgtga ggatatatat gcaattaaca gaggtaaaag agaattaagt
    28501 aaaaatgaaa ttaacaatgt tgtaaaaaaa tatgagctag agggagaaca tctatatttg
    28561 cttaaaaata ttacacataa agataagtta tttgatatag catgcaatga atgcggaagt
    28621 aaaaatgtag ctataggaat gtttgatgta gcaaatgaca tcatagaatt tagatgcaga
    28681 aactgcaata tgatagatgt agtaagtgta gatttctatt gagaagattt aaataattta
    28741 aatagtcaaa gtgaagttta taaatgaaac tagaatgtta tagacttact ttgacttata
    28801 aaaaggagtg tattaaatgg ctaatatata ttgcgaaaat tataattgcg aaaattactt
    28861 tgaagatatg tgtatgcttg aaagaattga aattaataac ctgaaagtgt gtgaaagtta
    28921 cattgaaggt aaaaatgagc tatataaatt agaaaacgga tatactatac atcctaaaga
    28981 tttgaaaatg gtgaaaagta aagattattc tgttgaagtt actcatattc caactggtat
    29041 tacagtaaaa tgccgttcta caaatagtat tttaaaaaat aaaaataagt gtttggaagt
    29101 tctagaagaa gaattaataa aaataaactc tcacttagag ctagaagatt tacgctaaat
    29161 aggaagtgat tttatgaaac gaagaagatg cagttggtgt ggcaagttat tttatcttaa
    29221 ggaaaaatct aaggatgttt attgttgtaa gaaatgtgag agaaaagcta agaaggtgaa
    29281 aaatgaaaat taattttgta atagatggag aaccaaaggc gaaggcacgt cctagaatga
    29341 gtacggcaaa tggtaaggct tatacacctg accaaactgt actctatgaa aattggatta
    29401 gactcatgta caactctaca gtaaagcatt tctttgaggg taatgtgaaa atgacagtta
    29461 tttgttactt tgacattact aaaaaagata gagaagcact aaagaaaaat aaggtaaata
    29521 ctaaagcgta taaggatgct ataggcaagg tagaagggtt aataagacca aacaagaaac
    29581 ccgatttaga caatataatc aagtctgtag ctgacagctt gaatggtata gcttataaag
    29641 atgattctca aatagtagaa gtagtgagta agaaatatta cagtgataga ccaagagttg
    29701 aagttgaatt ggaggatatt atttaactaa caaaaaaatc cgttcgttag agtatagact
    29761 tagtttttca taaaaatgtg aaaaagttaa ataaagaata tatcaaatga taaaggagag
    29821 ataggttatg aatgaaaata taaataaaga aataacagta cttggaactt tagaaatcga
    29881 gggaatgaaa tttcataaca ttgagggtgg atttggagaa cataagaaag caatgctagt
    29941 aaaagatata gctgagatac ataatagaga acttaaacat ataaatgagc taattaataa
    30001 taatataaag agatttaaaa caaatataga tattatagat ttaaaagtga gtcgttctga
    30061 acgactaggt gatttaaagg ctaatccttc aaaaggatta ggttgcgagg atgttgggta
    30121 tagtaaacaa tcttttaatc agtcaagaaa tgtttacttg ttatctgaaa gaggttattc
    30181 aaaactactt aaaatattgg aggatgatat agcttgggaa caatacgaga aaatagttga
    30241 tggatatttc tctatgagaa aagaattaaa taatcctctt ttaagtgcat caaaggagtt
    30301 acaggctata tttatgctag ataagaaaca agaagtctta gaaactaaaa tagagaatgt
    30361 taatgagaag ttagagaact ttatggatga tgcaccatta ttcaatatcg agtgtgagtg
    30421 tattgttaaa gaggttaaga gagtagcaac aaaatcactt ggtggacatg gaagtaaggc
    30481 ttataaaaat aaatctttaa gaggtaaagt ttatagtgat atataccatc agattaaacg
    30541 agaatttgga gtagatagtt ataaggctat aaagcgttgt caattagata aagtattaga
    30601 gattgtaaat agttataagt tacctatagt gtttgaagaa gaaataagac ttttgaatag
    30661 tcaattatca attgtgagtt aagacaggaa tagatttttc tcctttaaag gggaaaacct
    30721 gcaatagtta attaaaagag aaaaggagtg ctttcacact ccacttgtca aaaatataaa
    30781 acttttatcc aagattatta taacataaac aggagtgtgg aagtatggat aataatatta
    30841 ataaaaaaga actatttaaa aaagtagaag gtagactaca tcattataaa tttttaagtg
    30901 cagaaattaa aaatcttgaa ttagatatag aaagtagaga aaatgaaata tttggttgta
    30961 aggctatcgg gtatggtgaa aaagtaagtc caacatatgc ttttaattca actgttgaga
    31021 atgagattat aaaaaaagaa agagatattg ctagattaaa aaaactgaaa aaagataaag
    31081 aaatagaaaa gaagaaaata gaaaatgcac ttacatgctt agatataaga gaagaacatt
    31141 tttttaaact gttttataat agcagaatga aaaatagtat ggtttatata tccttagaaa
    31201 tgaactcaga taggaaaaca tgtagatgtg taagggaaag attagtgtat aaagttatgg
    31261 atatgcttta tccaagaatt aaggaaaatg aactcccatt atttaaaaat tagaaaattc
    31321 cccagttttt ccccagaaat tcccacttta ttccctactt tctccccgtt ttgattaaaa
    31381 aagcatgaga taatagtatt gtggaaataa agatttccct ctcaaaactt aatatttgac
    31441 tagggttaag ggattgccct agtcactatg aacagactag gcagggcatg aggatgctgt
    31501 tagttcaatt ctaactatgt tcaatcttta gtttttctat ttcaattaat ctacggatac
    31561 actaaaaaat agtatatgaa ttgagattaa aatctcatac aattttgtat cttaattcaa
    31621 agtctaaaaa ccgagtgggg cttggtaacc tcactcacca tgcaagtact ggtttaatct
    31681 aggttcgatt cctggaactt gctcccttta ataatatgta tcccccatta aaaaggctta
    31741 gattaacttc taggtctttt ttaatacaaa aatttattaa aaatgcacgg catgcactat
    31801 tcagatatgc atgtcatgca tgtctaaagt cgaatattta atattttcga tggctcaaat
    31861 tgaggggtcg aaaataaaaa caggaggtag tagtatgttg aaaattttac aagagaaaaa
    31921 tgtaaaagta atgtggtcca aaaatggaga agaagtttgg tttaatgcaa atgacgtagg
    31981 agaggaacta ggcatagtaa atattcgtga tacattaaga aatatagata gagaatataa
    32041 aaagaaattt aatgagtcta ctgtcggaga ttcctacact agaaacttta aagataaatt
    32101 gcctaacttc ggtactactt ttgttacaga agaagctgtg tacaatatgt catttagaag
    32161 taataaacca gaagcaaagt tatttacaaa atgggttaca aaaacactta aacaaattag
    32221 aatacatggt tattatattg ctacagaaaa agaccaggaa tggctggata taaggacaga
    32281 aggcaaaaaa gtaagaaaag attttacaga tgaaatacaa gagtttgtat attatgctac
    32341 tagtcaaggt agcaataaac ctcagatgta ttataaacat tttactgaac ttgtaagaaa
    32401 aaaattaggt attccaaaag gtgtgaaaag agatgagtta aatcaaagcg aactatttga
    32461 tatacaagca cttgaaagaa ttatatctat gaaattacct aagttaatag ataaagatat
    32521 gaattataaa gaggtatata aaaagattaa ggaattaata gaaatgattt aagggactgt
    32581 cttgatggag ggtctttttt aattcccaaa acgacaaaca aacgaggtgg tgatgtgcaa
    32641 gatgtcaaag aaaaggtaaa acaagattac ataaaaggaa tgaaacaaaa ggaaatatca
    32701 tcaaagtatg acattagttt aaacacttta aagtcatgga taaaaagata caactgggct
    32761 agtgaaaaaa agaagggtgc acctataaat aaaagaggtg cacccttttc taataaaaat
    32821 tcagttggtc atggtgctcc aaaagagaat aagaatgctg aaaagtttgg tttcttctca
    32881 aagtatctac ccaaagaaac tagagaattg atacaagaaa tatctataaa agataaattt
    32941 gatattcttt gggaacagat aacaattcaa tatgcagcaa taataagagc acaaaagata
    33001 atgtatgtta aaggcaagga agaaatggtt aaagaattaa agaaatatga aagcacagaa
    33061 aatggtgaaa agatagagta tgaatttcaa tttgcatggg ataggcaagc atcttttctt
    33121 aatgcacaga gtagagctat gagtgaactt agaagtttaa ttaaacagta tgatgaaatg
    33181 attcataagg attggaattt ggctacagag gagcagaaaa caagagttga gaagttgaaa
    33241 tgtgaagttg ataacctaag taaagatgat attggagatg atgagttgaa aataagtgta
    33301 gattatggtg atagaaatga tagttagagt aaattttaat ccagatttca aggaagctaa
    33361 ttttactaaa aaaagataca gagcaatgaa aggttcagca gggagtggaa aatctgttaa
    33421 tgtagcacaa gactatatac taaagttagg agataagaag tatcaaggag ctaatctatt
    33481 agtagttaga aagtcagaag ctacacataa gtattcaacg tatgcagagc ttacaggagc
    33541 tataaatcgt atttatagta aacaagctga taagtattgg aaaactactt taaatccttt
    33601 agaaattaag agtaaagtta ctggtaactc tataattttc agaggagtta atgatgcaaa
    33661 acaaagagaa aaattaaaat caattaactt ctcgaaagga aaattaacat gggtttggtg
    33721 tgaagaagct acagaactta tggaaagtga catagacata ctagatgacc gtttaagagg
    33781 tattttaact aatcctaacc tatactatca aatgacattt acatttaatc cagtctcagc
    33841 tactcattgg ataaaaagaa agtattttga ctataaaaat gatgatatat ttactcatca
    33901 tagtacttat ctacaaaata gattcataga tgaggcttac tacagaagaa tgcaaatgag
    33961 aaaagagcaa gacccagaag ggtacaaagt ctatggtctt ggagaatggg gagaaactgg
    34021 tggagcaata cttaaaaatt atgttataca tgaatttcct acagaatttg agtattttga
    34081 caatatgagg ttatcacaag actttggatt taaccatgca aatgtagtac ttagaattgg
    34141 ctttaaggat ggagaattat atatatgtaa cgaaatatat gtacatgaaa tggatacctc
    34201 agaaatcata aagattgcaa ataataaagg tttagaaaag aatctattta tgtactgtga
    34261 tagtgctgaa ccagatagaa ttaagatgtg gaagagtgca ggatatgaag ctaaaggagt
    34321 taaaaaagga ccaggaagtg ttaaagctca aatagattat ttgaaacaat taagaataca
    34381 tgtacatcct agttgcacta ataccataaa agaaatacaa caatggaaat ggaaacaaga
    34441 tgaaagaact ggattatatc ttgatgaacc agttgagttt atggatgatg caatggctgc
    34501 gcttagatat tctatagata ataagcttaa aaataatgga ataagcttct taaagtaaag
    34561 gaggcgttaa atatttatat aagtgaaaca gatttaataa aagttcagtt aaaaaaagag
    34621 agcaccttta acctagtaaa agttatagag cattatattt taaaacatag accagagaaa
    34681 tataaacaag gtgaagaata ctattatggc aatgcagata taaacaataa gagaagatat
    34741 tatctcttag atggagctaa ggttgatgat tttactaaag ttaataataa agcaattaac
    34801 aactaccata agcttttagt tgaccaaaag gtaggctata gtgtcggaaa tcccatagta
    34861 tttaatgcag atgatgataa tctcactaag cttttaaatg acttactagg agaagaattt
    34921 gacgatacaa taacagaact ctacctcaat gctagtaaca aaggtattga atggttacat
    34981 ccatatatta atagaaaagg tgagtttaaa tatgtaataa tacctgctga agaagcaatt
    35041 cctatttggg atagtaaaag acagagggaa ttagttgcat ttattaggtt ttattatatt
    35101 gaagatatag atggaaataa aataaaaaga gttgagtact acacagaaaa tgacgtaact
    35161 tactttattg aaagaggtaa tagttttatt caagaatttt tatatgatga atatggaaaa
    35221 atgactgata tacaagaagg tcattttaga ataaataaca aagaacaggg atggggtaaa
    35281 gttccattta taccttttaa aaataatgaa aagtgcgtat cagatttaaa tttctataaa
    35341 tcattaatag atatatatga caataatatt tctacactag cagataactt agatgaaata
    35401 caagaggtta tttatgtatt aaaagaatat ccaggaacaa gtctacaaga gtttatagat
    35461 aatataagat actataaatc aattaaagta gatggtggag gtggagttga taaactagag
    35521 ataaatatac cagttgaagc taaaaaggag cttcttgata gattggaaaa gaatataatt
    35581 atctttggtc aaggagttaa tccagaatct caaaacacag gtgacaaatc tggtgtagca
    35641 cttaaatttt tatattcact acttgattta aaatgctcca aaactgaaaa gaagtttaaa
    35701 aaagcaatta gagaactttt gtggtttgta tgtgagtatt taaagataag tggtagtaaa
    35761 agttatgatt ataaatcagt tcaaattact tttaaccact caatgataat aaatgagtct
    35821 gaaaaaatag atatggcagc taaatcaatt ggaatcatat cagatgaaac tattgtttcc
    35881 aatcatcctt gggttgagga tgttaatgac gaacttgaga gacttaaaaa acaggaagat
    35941 actcaaaaag agtatgatga tttaattcct aataatcaag atggtgttat agatgaaaca
    36001 taaagattat tggagaaaga gatttgaaca attagaagaa gctcaaaata acaaaagtgt
    36061 aaaatattat cttgaattag aaaagcaata taaactagct ataaataata tagaaaaaga
    36121 tatattagca tggtacaaca gatttgccaa aaatgaagga atatctttat tagaagctaa
    36181 gaaactacta aatacaagag aactagaaga gtttaaatgg agtgtagaag aatatattaa
    36241 atatggtaaa gaaaatgcta taaatcaaaa gtggatgaaa gagttagaaa atgctagtgc
    36301 aagagttcat ataacaaggc ttgaagcttt aaagttacaa atacagcaac aagtagaagt
    36361 tttatatgga aatgaacttg atggtattga taaactaatg agagatattt atacaagtgg
    36421 atactatcat acagctttta atgttcaaca aggagtaaac gttggttgga gtttaatgag
    36481 tcttgatact aatagaataa ataaaattat ctctaaacca tgggcaacag atggattaaa
    36541 ctttagtgaa agaatttggg gtaagcatag acatgcttta gtaaatgaac tacatactaa
    36601 gctaactcaa tcaattatta gaggtgaaaa tccaaagaat ctagtaaatg actttgctaa
    36661 aagatttaat gtatctaaat ctcaagctaa gaacttaata atgactgaat cagctttctt
    36721 tgcatcagca agtagaaaag attgttttaa tgatttagat gtagagaaat atgagataat
    36781 tgctacacta gatttaagaa cttcaaatat atgcagagaa ttagatggta aagtatttga
    36841 tatgaaagat tatcaagttg gaataacagc tccaccattt cattgtcgtt gtaggacaac
    36901 aacagctcct tggtttgaag atgaagaagg ttacagagca gcaaaaggag aagatggaaa
    36961 aacatattat gtaccatcta atatgaagta taatgagtgg tatgagaaac atatagaggg
    37021 tagacttgga aaagaaaaag ctgataccct tagaaaaatg caactaaatg agagtaaaga
    37081 tagaaaacaa tttgaagaat ataaaaaggt attaggaaat gaaatacctt ctaaatttga
    37141 tgaatatcaa catatgaagt ataatgatac tataaggtat gaggaagcta aaaagcttta
    37201 taaggatgta aattggcaag ttaagaatca gagaaattta actagtggaa gtgttcattc
    37261 agtgccattt gaatcaaaac caaatagtgt atttgataat tacaaagatg gtaaattaat
    37321 tcaaagaaga tactatggta atacaggaaa acctagatta gatttagact taactgacca
    37381 tagaaaccca aagcaacata aaattgtacc tcataaacat gattggttag ctgatgaaaa
    37441 taaccatgat aaggtaaaaa gagagaagga tatggagctt acaaaagctc ataaaatagc
    37501 taacaaagat attttgaaag gagagtaaaa tggataataa atttaagaat ttacaacatt
    37561 taattgattc tattgattta ggattagata tagaatttga cttatataat aaaccatata
    37621 atatatctat tggagatgat gatacaaggt ttattactct ttgccctaat ggagatacaa
    37681 agtattataa aaatggtaag gatatggttg ataattacaa cattgatggt aaattactaa
    37741 aggatttatg gaaagatata caaatagtaa atatgtaaaa gcacttgcta aatgataaat
    37801 tagtaggtgc ttttattatg taaaaaatga aaggagatat ttaaaagatg gattggttaa
    37861 aagaattgct agaaggaata aaaatagaaa ataacaaaat tgatgtagtt tctcttcaaa
    37921 aatctataga aaagaaaata aaagagacta caattactca agaagattat acaaatcttg
    37981 aaacacaact taatacagct aatgaagcta ttaaaaagtt tgaaggaggt atgacaaaag
    38041 aagatgtaga gaatctaaaa acaacttatg aaactgataa gaaaactttg gaagaaacct
    38101 acaaaaaaga aattgaagaa aaggacttta attattggtt aaatgatgct tttaagtcta
    38161 ttaaatgtag ggatgaaata gcgttaaaag ctcatttaga tatagaagca ctaagaaata
    38221 gtaaagatag acaaaaagct tttgaagagc aaataaaccc tttgaaacag gataaagatt
    38281 atttgtttaa tgcaacacta gaaggtgaag agcctaaaat agatactata acaccagggc
    38341 aagagcctaa gataaatgat tttggtttta attttactgg ggtaagacct catgaaaata
    38401 ataataaata ggaggaaata aaatggcagc actaaattat gcaaaagaat attcaaatgt
    38461 tttagcacaa gcatatcctt atactttaaa cttcggggat ttgtatgcaa caccaaataa
    38521 tggaagatat agatggactg gttctaaaac aatagaaata ccaactatat ctacaactgg
    38581 aagagtagat tcaaacagag atacaatagc agtagctcaa agaaactatg ataatgcttg
    38641 ggaacctaag gtattaacta atcaaaggaa atggtcaaca ttggttcatc cagcagatat
    38701 aaaccaaact aattatgtgg cttcaatagg caatataaca aaagtatata atgaggaaca
    38761 aaagtttcca gagatggatg cttactgtat atctaaaata tatgctgatt ggaccgcatt
    38821 aggtaacaca gcagatacaa ctgttcttac aacaacaaac gtattagaag tatttgataa
    38881 gttaatggaa aaaatgacag aagctagagt acctgaaaat ggaagaatat tgtatgttac
    38941 tccagtagta aatacactta tcaaaaatgc aaaagagata caaagaacag taaatataaa
    39001 ggatgcagga acttctctta atcgtcaaac aactgatatt gacacagtta aaataattaa
    39061 agtaccatct aatctaatga aaactgcata tgattttaca actggatgga aagtaggagc
    39121 aggagctaaa caaatcttta tgtccttagt tcacccaagt gcaataatta cacctgtttc
    39181 ttatcagttc tctaagttag acgaaccaac agcagttaca gagggaaaat acttctactt
    39241 tgaagaaagt tttgaggatg tatttatatt aaataaaaaa gctgatgcaa tacaatttgt
    39301 tgttgaagga gctggagcat aatggcacaa gtaaggaaat taaatagaat attaactata
    39361 gaagaatgta aaatagatga tttcttagag atgggatatg atttgataga tgaaactggt
    39421 aaggcagtaa agtatggcaa gtcattaaat gtaaaagatt taatagctga aaataatatt
    39481 ttaaggtcaa aagttgagtc tttagaagaa gaaaataaac agcttaaaga gaaaaataaa
    39541 cttactaaaa agtaggtgaa aattatggga aataatataa ttgatgatat agaaaaaaga
    39601 cttgaaagtt ttggatatat attaaaagat ggggataagt ggttaataga ttttgtaaga
    39661 gaaaaaatag aaaatattat taaactagat tgtaatataa aaactatgcc aattgaattg
    39721 aaagaaattg aagttgatat gatagttgga gagttcttat ttaccaagaa aaatatgggg
    39781 caattagata tagaaagcat taactttgaa gctgtagaaa agtctatatc agaaggtgat
    39841 acaaaggtag attttgctat aggaagtggc tctcaaacac cagaacaacg ctttgatagc
    39901 ttagtagctt atcttactac ttatggtaag aataagatat taacctttag gtgcttaaga
    39961 tggtaagtaa aactagaaaa gcaatagaaa tgttatatag agataaatgt actatagttg
    40021 agtatcagcc aattaaagac cctgtaacaa aacgaactaa caataaagaa gtgattgtat
    40081 tagaaaatca accatgtaaa ctttcatgta aaaatatagt ttctgctaca gaagggaaag
    40141 tagctaagct agagcaaact attaaactct ttatatctcc agatatagaa attaaagcag
    40201 gttcaaaact tattataaat gataaagagt atgtaagaag tggagaatca gctatatatc
    40261 caaatcatca agaaataata cttgagttat ttaaggataa agcataatgg ctagatgggg
    40321 cagtgttgat tttagagagt ttaaaagagt ttgtaaaaag atggaggagc ttacaaagat
    40381 tgatttagat aagttttgca aggatgcagc aagagaatta gcagcacgat tacttgggaa
    40441 agtaattaga agaacaccag ttgatacagg attcttacga caaggatgga atggagtggc
    40501 ttatgctagg tcgcttcctg tgtataaaca aggaaataat tatattatag aggttgttaa
    40561 tccgaccaca tatgcaagtt atgttgaata tggccataga actaaagatg gaaaaggttg
    40621 ggttaaagga caacatttct taacaatttc agagatggaa ctacaaagcc aagttgataa
    40681 gattatagag aaaaaactat taatattgct taaaggagta tttgatgctt aataatataa
    40741 ttgatggaat atctattaaa ttagataaaa catttggaga gagttataca atttatagtg
    40801 aagatgtgga gcaaggtata aatgaacctt gtttttttat tgttccttta aatccaagca
    40861 aagtatccta tctaagtggc aggacattaa aaaagaactc ttttgatgta cattattttc
    40921 ctaaaagtaa tgataaatca tttgaaataa atgagatagc tgagatgtta ctggaggaat
    40981 tagagtatat agaaattgat ggagacttag tcagaggtac aaatatgaac tttgaaatta
    41041 tagataatgt tcttcatttc tttgttgatt ataactactt tactataaaa agtaatgaca
    41101 cagataagat ggatacagta gagttattcg gtggtttgaa gagaggtgat aattttgagt
    41161 aaaacattaa gcaaagaaga taactacaag tttactaagg agcagatagt taactctaag
    41221 aagtatgtaa atagaaaaga tttattaaat gcaattttaa aagaaaatga tttatattcc
    41281 ttctcagagg tagaggatag aataaataaa tttatgaaag gagtgagtta gatggcttta
    41341 ggtggaggaa catttgtaac acaaaataag gtcctacctg gtgcatatat aaattttgta
    41401 agtgctacaa gggcaaccag ttcattatcg gatagaggta ttgttgcaat acctttagag
    41461 ttagattggg gcatagataa agacgtattt caagtaacca gtgatgattt tgagaagtat
    41521 tcagtgaagt attttggata tgattatact catgagaagc tgaaaggttt gagagattta
    41581 ttcaaaaata taaggttggg atatttttat aaattaaata aaggcgttaa agccagttgt
    41641 actatagcca cagcaaaata tagtggtatc agaggaaatg acttaaaagt aacagttaca
    41701 acaaatatag atgataacac taaatttgat gttgtaacac ttttagataa taagaaggta
    41761 gatactcaaa tagcaaaggt tattacagac ttacaagaca atgactatat cacttggaag
    41821 aaggatgcaa cactagaagc aagtgcagga cttgtattta ctggtggaac taatggcgaa
    41881 gctgtgacag gagcagagta ccaagctttc ttggataaaa tagaaagcta tagctttaat
    41941 gctttaggat gtttggctac aacaacagaa attaaaagtt tatttgtaga atttacaaag
    42001 agaatgagag ataaggtagg agctaagttt caaacagtac tatataagaa aagtgatgca
    42061 gattatgaag gtgtagtgtc tgtagaaaat aagattaaag ataaagattt agttgaatct
    42121 agtttaattt attgggcggc tggagctata gcaggatgcg atataaataa atctaatact
    42181 aataaaaagt atgatggtga gtttgatgtt gatgttaatt atacacaaat acaacttgaa
    42241 gaagctttaa aaactggtaa atttatattc cacaaggtgg gagatgaagt tcatgtgtta
    42301 gaggatataa atacttttgt atcatttaca gatgataaaa atgacgattt ttcaagtaac
    42361 caaagtgtta gagtacttga ccaaattgct aatgatattg caactttatt taatgaaaag
    42421 tatttaggta aagttccgaa tgataaggca ggaagaataa gtttctggaa tgatgttgtt
    42481 aaacaccata aagaattaga gaatataagg gcaatagaag attttaaaac tgatgatgtt
    42541 agtgtagagc ttggaaatga taagaaaact gtcatagtat ctgatgctgt taaggttata
    42601 aatgctatga gtaagcttta tatgacagtt tcagttagtt agagagggga gtgataatat
    42661 ggctcaaaca ataaatgcta aagatacagt tagtgcaaag aaagctgaat gttttataac
    42721 tatagaaggc aaaagatata attttatgca agctatagat ttagaggcta aaatggaaaa
    42781 aaataaaagt gaagttccaa ttctaggaag aacaacaaag ggaaataaaa caactgggag
    42841 tacaaatact ggaagtgcaa catttcatta taatacttct atttttagag aattacttta
    42901 cagatataaa gaaactggtg aggatattta ttttgacata caagttacaa atgaagaccc
    42961 tacatctgct gtaggaagac agacagtagt acttaaagat tgtaatatgg acagtggaat
    43021 aattactaaa tttgatgctg atggtgagta tttagatgaa gatatggatt tcacttttga
    43081 ggattgggaa ttagtagaaa aatttaattt attggcagga atggagtaaa atacacattt
    43141 ataaattata tatgtgtatt ttttatatga aaaattaaaa taaaaggaga ttagaataat
    43201 atgagtaatt taagtgcttt tttaagtcaa aatgcaataa aggttgataa tgtaaaatat
    43261 gtagcgagta acagattttt agataaagaa gggaaaccag ttgaatggga attaaaagtt
    43321 ttatcatctg aagaagacga agcactaaga agaaagtgta ctaaaagagt aaaagtgatt
    43381 ggtaacaatg gtaagcatac tggacaatat acaagtgaaa ttgactacaa tagttatgta
    43441 gctgaattat gtgtagcatc tacagtattt ccagatttaa aggatgccga actccaaaat
    43501 agttatggag taatgggaga agctcagtta ttaaagacaa tgcttacagc aggtgagtat
    43561 gtcaattata cagtaaaagt gaatgaagtc aatggatttg atacatcttt tgaggataaa
    43621 gtagaagaag caaaaaacta atcagaggtg gcgattttga tgctagcatc actcattatt
    43681 gtattcaaaa attaaagtgg aagccaagtg aatatatgaa tttagaagtt aatgagagag
    43741 cgttagcagc cgcctcaata cttataaaga tagaagatga agaggaagca atgaaagaag
    43801 ctgaaagaga gagaaagagg ggacgaagaa gatagcaaaa taaaaaaata aatatagaat
    43861 aggtaaaata tgtaataatt atatgttata atatttttag caagaagatg taatctacaa
    43921 tttatagagt ggagttcata ctgagataaa acctacttcc taatgaaagg aggtgggaag
    43981 tatgaataac tttttactta atgtaatagc tggcgttatt gctagtttaa tattttgctt
    44041 aatttgtaaa gtatttctaa aagtaaaaag ccactcaact cgtggcaaga gtaaaagtgg
    44101 ctgggaattt gattttaaaa tcaagttcca taagttcaaa tagattcatt taattatgaa
    44161 cttcactcta ccgcaaaata gattgtagtt cttcttgctt ttattatacc acaaattaga
    44221 aaaaatattg tttatataaa ataaaaaata aaaattttta ttaaaaaatt gaaaacttga
    44281 ttataaagca attaatttat aaaatatata taaataagta ggtatttatt tacttgaatt
    44341 tcattgttta tataaaaaaa atggaaaaat atgtaataat tatatgttat aataattgta
    44401 gcaaggataa taatcgaaag tgcgaagggt gattattttc atattaaacg ccaaattcca
    44461 aataaggaag gaggtgaaat tatatgatag tttttttatt aagcatacta gctggtgtta
    44521 tatcagctta tatttatgac aaaataaaaa atcacccaga cgccaataag ggtgatttaa
    44581 aaaaataatt ctttaaatca attttgatgg aaatagctac tcttgtataa agtaaattat
    44641 ttccttgctt ttattatacc acaaattggt acagatattc aaaaataata tttttatgat
    44701 ataataaaaa tgtagagatt ttgcagtgag caatatttgc gataaattga agtttaacaa
    44761 ttggaataca aggtattgag ggtgtgtgat aaatgttatc aattgcacta ctcatggttc
    44821 actgcaaatt tgagagaggt gtgtatgtgt agatattgga aatactaagt ttattttggg
    44881 gttttagatt aactatatgg aatgtaaatg tattactttt cactgactac ttttcccctg
    44941 ctgtgcgttt tagattaact atatggaatg taaatctaca actttagtta gattaccttt
    45001 catatcaact tgttttagat taactatatg gaatgtaaat cttgtttttc aattatttta
    45061 tctacgcagt ctatacagtt ttagattaac tatatggaat gtaaattaaa aaatacaaag
    45121 aagaattgta caatgaattt tctgttttag attaactata tggaatgtaa atgttgcatt
    45181 tgtatatcct gtaccatcac caattattgt tttagattaa ctatatggaa tgtaaatttc
    45241 gcaacttatg atggtgaaat gattacatta acaggtttta tattaactat gtggtatgta
    45301 aagattactc taaaagtctt tttatcattt tctacctcaa ttttatatta actatgtggt
    45361 atgtaaataa agcaaaagat aggaatgata attttaaaac tccaaacttt tatattaaca
    45421 atgtggagaa aaactaaata gaagaaagaa gcacttattt ttggtaggtg cttttatttt
    45481 gctcaaaata ttttaaatca atagactaag ttcttatttt tagatagaat tatatttgaa
    45541 taaagaattt aataggggag agttcattat gtggggaaaa tttaaaaaat taagtttgtt
    45601 gaagaaaatt ttagtgatat ttttaatata ctttgttgta tttacagtat caatgatgat
    45661 tcatcaagcg atacgagatt caaaaaatag agatgaagta aatgaaggaa atattacaaa
    45721 agaaaatata attagcgaaa gagaaaaaga agatatttat aaacaagaga tgcaagcaaa
    45781 agtagactct atgataccag aagacttaaa agataaaaca acatattatg ttaacatatt
    45841 aaacccaaca aaaggtgagg gatatatagt tagtattcaa gtggaaaatt ctaggtttaa
    45901 tgatgaaaat gagtgtagaa actttactaa agaatttgta aacaatataa aagatatgaa
    45961 tgatatccat tcagtgagaa taagctttat tgttgatgtg acactcactt ataatgtatt
    46021 tttagatgat tggaataata taaaaaataa tgtaaattta attgatgatt tggatttctc
    46081 gtctggaaat taggtgaata ctttatttta aaattaaatc tgttatatag aaagcactta
    46141 ctttttggta ggtgcttttg ttttgctcaa aattggtcgg ttgagtaaaa taattagaaa
    46201 aaattagtaa aaaactcttg aattattgta cgtactatat tataatttaa gtacggacaa
    46261 taaaaagaga ggtgataaga tgtccaataa attaggcaga ccaccaaaag aaaattccaa
    46321 aaaattaaga tttgaagtta gacttaacca agaacaagca gatatattaa atgagtgtgc
    46381 tgaaaatctt aaaatatcta aaacagatgt tgtaattaga ggaattgaat tggtgaaaga
    46441 gaagattgac aaaaacaaat aaaaaacagc cgctgcaccg accaaagcac ttgcgactgt
    46501 ttcccaaaga agttaccttc tatgaaatat attctatcat agtaaggtac ttcttacaat
    46561 caaatttaag gaggaattta ttatgaatga attaatgaat tttgaaggaa aagatataga
    46621 ggtattcgaa tttgaagggc aaattttatt taatccaaag catgtggcag agtgtttaga
    46681 tatttcagat gtgaacagta gtattagaaa atttaatgat aatcaagtag ttaagttgac
    46741 taattctaaa atgcataata tgcagtttag aaaattgcat aatacaggag aaaattttct
    46801 aactgaaagt ggtgtttata aacttatatt taaatctaaa aaagaagaag ctgagagatt
    46861 tcaagattgg ataagtgatg aagtacttcc agccattcga caaactggtg catacataac
    46921 aaataatgct gaccccgaaa aactaagaga aaaagcaagt gagattgaaa aattacaact
    46981 ggcttataac agcacatcta tgttaaaaga actattggat ggtgcaggtt ttgacaataa
    47041 atctaagtta cttacagcta aaacattata taaaaaggca ggaattgatt tgccaataga
    47101 gataaacgaa gaagaacatt attttgacac aaaacaaata gcatctaagt taaaactata
    47161 ttctaagagt aataaaccag ctcagatggc tgtttgtgag attattaaaa agattgattt
    47221 agaagatagt gaagttaagg gagtttggga gactaatgga agttggactg gtactgtaaa
    47281 taagtataca gaaagtgtaa tagataaaat aagaaattgg atagaggaga ataatagacc
    47341 taccaagatt gcaggtgaga agaagaatta tcatgtggtt tataaaattg agtaaattta
    47401 tcagttgtat taaatatttt agtttagttt tggggggatt aatacaatgc atgagaattt
    47461 acttgatata gatagaatag aacttatcaa agaacttgga aatatctttg aaaaaatgaa
    47521 aaatgaaaat ccagatgaat tttatagatt tgtaagttta gtgaaagaag aatgtaggaa
    47581 aaaaagagaa aaataaatag acaaataaag cacttgaata ttttactgtt tcaagtgctt
    47641 tatgtagtaa aaaatgatat aatataggta ggagttatat taactaagtg gtatgtaaat
    47701 gctgtatcct ttgccccttt tgctgcgtca ctcaaggttt ttatattaac aatgtggtat
    47761 gtaaagtcgt ttatcattgc tagcctcgca ttcgtactgg tcaattttat attaactaag
    47821 tggtatgtaa agattcgcga tactatttgt gcagtatcta agtaggatgg ttttatatta
    47881 actaagtggt atgtaaatca ttcactacaa aatatatatt ctaatgtatc attcgtttta
    47941 gattaactat atggaatgta aataatatag tgaccacttt tagctttttt gttagtgttc
    48001 gttttagatt aactatatgg aatgtaaatt catcattcac ttttgcaaca tgtatgtttt
    48061 taagaccagg ttttagatta actatatgga atgtaaatta ttttacagat gaacaattac
    48121 agttacttct tgaatgtttt atattaacta agtggtatgt aaatgtaact cttgttgttc
    48181 tataggtaaa tacacctgtg ttttatatta actatgtggt atgtaaatct aagagtaatt
    48241 tttttagaag gtctttaggg gttgcgtttt atattaacta tgtggtatgt aaatgtacta
    48301 gaaaatgcac tacctgcatt cattcctata cggttttata ttaactatgt ggtatgtaaa
    48361 ttagttagtc ccatatcgtt atggtactgc attaacgcgt tttatattaa ctaagtggta
    48421 tgtaaatata ttgagcttca tttttggggt agaatctagt gtatggtttt atattaacta
    48481 tgtggtatgt aaatgtacaa atgcacgatt agcaatgata aatgatgctc gttttatatt
    48541 aactatgtgg acttaaaatt aaaaataatt caaaaacact tacaaatgag taagtgtttt
    48601 ttttatgaaa ggaggtgata ataatgtaaa aattttacgt atatagtata atagtgctat
    48661 aaaataagta ttatgtgagg tgattgttgt gttttgttca aattgtggtt atgagataac
    48721 tggtgcaggc aaattttgct caaattgcgg aacagctaca ttagcagata aagttaacaa
    48781 tgatgattta tttataaatg ttcatggaaa agaattaaat ctgactaata tttataaaga
    48841 aactaaagga gataaaatct tagcaattga tattgcaatg aagttactag ggctggacat
    48901 aaaagagtgt aaaaatatta tatatccagc ttttaaagaa ttaagtgaaa aaataaatat
    48961 cgaagaggaa aaagagatat tgagggaaga ggagtataaa caaactaatg tacttgaaga
    49021 tgatgttgct cgttgcccta ggtgtggctc tgtttcattg tctgctcata agaaaggttt
    49081 tggcatagga aaagctgtag caggggctac tatagcagga ggcattggtt tagtagctgg
    49141 aaatttagga gcaaagaaag ttagagttac atgtttgagc tgtggtaaac agttttgggc
    49201 ataaataata aacacttact aatgtaggtg tttttttata tggaaattta tgaaaggaga
    49261 gtgaggaaat ggctacaata caaacttcaa taaagatttt cgacggaatg acaccagcat
    49321 ttcgtaatat gactaattct attaatacaa caattaatag tttagagaga ttgcaaggca
    49381 gattgaataa tccactcaat gcaggtaata tacaagcttc tcaacaaagt ttgaataaca
    49441 tagaaagtat tctcacaagg atagaacaga aaattggaag aaatacaaat gagcaggaaa
    49501 actttaataa taaaataagg caaggtagtg aagcaggttc tctattagtg tctaaattaa
    49561 aaagtattgc tgggatatac attggaataa aaggaataga aagtattaca aaagcagcag
    49621 atacaattgc aagtacaaaa gcacgtttaa atctaatgaa tgatggctta cagacaacag
    49681 accagcttaa taaaatgatt tatttgtcag cccaaagtgc aagagctagt tatgcagata
    49741 cagcagcaca gattgctaaa cttggaatac ttgcaggaga tgcttttgga agttcagcag
    49801 aggtgataaa gtttacagaa cttatgaata aagcttttgt aattggagga acatcagcaa
    49861 atgaagctag tgcagcaatg tatcaattaa cacaagccat gggtgcagga aaacttcaag
    49921 gtgatgagtt ccgttccata atggaaaatg cacccttatt agccactaaa atagctgatg
    49981 caatgggaaa aactaaagac caattgaagg aattatcaag tagcggagca ataacagcag
    50041 atgttataag aaatgcactg tttaaagctt ctgatgagat agaaaagaaa tttgcaagta
    50101 tgccaatcac tttttctcaa gctctcacaa tgatgaaaaa tgatgcttat atgatatttt
    50161 ctgagactct cagtaagata agcggagctt tgcaaagtgt acgttttagt gagattgttg
    50221 tatctatgcg gaatgttatg attgcaatat cttcaaacat ttatgataca ttaaatatta
    50281 taaaaaatat attaaatagt gatttttttt cagattttgt acaaggaatt acaacaggaa
    50341 ctgtactgat aatacaagga ttgggatgga ttactaatgc tgcactaaat gttgctaatg
    50401 tctttgctca aaattggagt attattgcac ctgctgttta tggagttgtc gcagctattg
    50461 caatatataa aggaatttta cttgcaagtg caattgtaac tggagtatca tcttttgtaa
    50521 atgccctgta tgctttaggg gcatataaag cttgtgcagc attggcagca caagaattag
    50581 ctttatttgg taaaatatct gcacaaacta tagaagcgat ggtaacagcc caggcaacag
    50641 cagcacaata tggcctaaat gcagcgttgt atgcttgccc gctcgtgtgg atagttcttg
    50701 gatttatagc agtaatagct gtaatattta tggtagtagc ggcaataaat aaatttgcag
    50761 gtacgtcttt gactgttcta ggggtgattg taggtgcagt atttgcagca gttgcaacaa
    50821 tacaaaatat aatgatatgg ctatttaata gatgtgtaga tgtaaatgaa ggaattgcga
    50881 atggatggaa tcagtgtgta tatttgatga aacaagcaat tgcaaaaggt gtaattttta
    50941 taattgagaa aatggcatca ttgaatgatt ctgtaaataa tgcaggaaac gcacttggta
    51001 aggcttttgt ggatggagca aatatagcaa taagaggggt aaataagtta attgacctaa
    51061 taaataaaat accagggata aatattggta aagtaggaga ggcaacgttt acgccagtta
    51121 aggcagataa tagttacatc aaacaacaga ttgatagctt aaacagatgg gtaggagacg
    51181 caccagagaa agtaaaattg gagcgaatgg gatacaaaga tattggagca gaatttcaaa
    51241 aaggaaatgc acttggaact aaatggcaaa atgctatatc taataaatta aaagatactt
    51301 ttgacattaa taagatgcta gaagatgcaa aagataagct aggattaaaa gatttgtggg
    51361 ataaagacaa tccacttaat aatcttggag gatttggtgg agatttagga aaaaatgtaa
    51421 aggacacggc gggaaatact gccaaaatgg ctaaaacaat ggataaaagt caagaagact
    51481 taaaatatct tagagatatt gcagagcaag aaacaataaa tagattcaca ggagtaaaca
    51541 ttaaaattga catgaacaat acaaacaaca taaatagtga ggcagatgta gatggaatag
    51601 taaatgtact aacagaaaaa ttaaacgacg ctatggttgt atcagctgag ggaatagttt
    51661 agagaggagg gatataaatg gcttatgact tttatttaga tggagtacaa ttaccaatac
    51721 ctccgccaaa gttagagatt aaagttacaa ataaaaacaa gacagttgat ttaataaatg
    51781 ttggagaagt aaatatatta aaaaaagaag gattatctga aataagtttt gaagcagaat
    51841 ttacacataa taaattacct ttttgtagag gtcaatttag agatgttcaa ttctttttaa
    51901 gtaaactaga attactaaaa actgattgta agccatttca atttattgta tcgagggaat
    51961 taggtaataa agtactattt aacactaata tgaaagtatc tcttgaggaa tataacatag
    52021 tagaagatgc agaaaatggc tcagatacaa aagttgcaat aaagttaaag caatatagag
    52081 attactcaac taaaaagtta gttcctgcaa ctcctgaaaa gacaaactat ggtaggactc
    52141 cccctccagt catgaaacca aaagaattta gaccagattc atccaataag ccaaatggta
    52201 aaacatatac agtaaaagca ggggatagcc tttggttaat ctgcaagaag caacttggta
    52261 atggttcatt atataagaaa gtatacgaac taaataaatc tatgatggat aaggcaaata
    52321 agggcaaaaa cttaagtaaa tacactattt acaaagggca ggtgttaaaa cttggctgat
    52381 gatttagttc tggcgaatga tagagatgta agattaatca tagcacattg ggaagatttc
    52441 tatgaacctg ctgtcattga tggtatcaca tgggaaatag agagacgagg aacaccatct
    52501 aagcttgaat ttacaatagt catggatgat atattagagt tttgtgaagg taactctgta
    52561 agactatatt acaaaggtgt aggtatattc tatggatata tatttcagaa gaaaagagat
    52621 aaagaaaatc acattaaaat tgttgcttac gaccagttaa gatattttaa gaacaaagat
    52681 acttatgtgt atagcaataa aacagcaagt gaacttgtaa aaatgttggc taaagatttt
    52741 aatttaaaat acaatgtcat agaagatact aagtataaac tatctagagt cgaagaaaat
    52801 aaaacactct ttgacatgat actaacagca ctagatgata ctctaagaga gaaaaaagaa
    52861 atgtatgtgt tatatgatga ttttggaaga ataacattaa agaatgttgc atcaatgaaa
    52921 ttagatactg ttatgaacaa tgatgtaata gaagattttg actataattc atcaatagat
    52981 agtgatactt acacaaagat taaacttgtg agagataatg aagaaacagg aaaaagagat
    53041 gtgtatattg ctcaagactc aacgcatatg aggagttggg gaatacttca aatgtttgat
    53101 acagtagaca aaaacatgag tgaagcagag ataaaacaaa agtgtgatat acttctaaaa
    53161 ctatataata agaaaactaa gtcattaagt ttaaaaaatg tacttggtga tattagagtg
    53221 agagcaggtt gtttagtacc tgttttttta gatttgggag atattgaatt acaaaattat
    53281 atgttagttg agaaagtaaa acatacattt gaaaataatt cgcactttat ggatttgacc
    53341 cttgttgatg gagacgaatt tgcttcatat tcttcaagct catatagtag tggaaatact
    53401 aataataaag atgaaaagaa aaatggtcct gcacaaagta ttacgaaaaa aaatacaggt
    53461 aaaaaagttc ctgctatatt tactgcatat tatccaggga acaatgcaat ggaaggcgga
    53521 aaaacagatt gcaatggaaa gccacttgat gtaaaatcaa gaactgttgc tggtccaatg
    53581 aatcgagaag gagttaagaa aacttggtat actgatgatt ttctaaagaa acatccagtt
    53641 tttgaatatg gagataaagt aaaaattata cttcctggta ctgcctatga caacaaagta
    53701 tatacagtta aagataatgg aggaagaata tatgttgaaa caaacggaac atatcatata
    53761 gatatactat tagctaatgc tagtgaatgt aaaaaatttg gtagaaagaa tggctatata
    53821 attataggtg gagatgaaga acaaacatat caagttgaag gtaataacca aagtagtaca
    53881 aataataact ctaaagaaga taaattaatt agtatagcaa aaagtaaact gggttgtaat
    53941 tatgtgtatg gagcagaagg tcctaataat tttgattgca gtgggtttac tcaatggtgt
    54001 tataaacaaa taggtataaa aattcctcgt accgcttctg cacaaagtaa agcaggaaaa
    54061 gcagtagatt taaaagatag aagcaagtgg aaagcaggag acttattatg tagaattggt
    54121 ggaggaagta gtaatcatgt tgtaatgtat attggaaaca atcaaataat tcattcacca
    54181 caaacaggag atgtggtaaa aatagagtct gttaattctt atagaaaagg aaaagcatac
    54241 acacatgtga gaagatttat ataagtgagg tgacaatatg agccaagatt tattacagat
    54301 aataaaaaaa gctgcaatgg atgcagtaga aacaagcaac ccaatgaggg ttgtatttgg
    54361 aacaatagaa agtattaatc ctctaagagt taagatagaa caaaaactat ctattggtga
    54421 aatttttcta atacaaacag atacatttaa aagatataca gataaaaaaa taggagataa
    54481 agtagtctta attcgtatgc aaggaggaca acaatattta gtattggata ggatgtgatg
    54541 aagtgttacc aagcgataat ttagattatg acattgaaga tgtatcaata attaattttg
    54601 atgtaaggca agaaccaagt aagaccttta aattaaatat agaaaaatct aagatagatg
    54661 gtatttgtga tgatgttgaa gcattaaaac aaaccatctt tttaatttta aacacagaga
    54721 gataccaaca tctaatatat agttggaatt atggagtcga gttgaacgac cttattggag
    54781 agcctatatc ctttgtaatc cccgaacttg aaagacgaat caaagaagca ctaattcaag
    54841 atgatagggt tgaaaatgta gataattttg agtttcaaaa tgtaaagggt aaagtacatt
    54901 gtaagttcac agtttacagt aaatatggaa atataaaagc agagaaggtg gtgagtgtat
    54961 aattgtttga gttaatgaca tttgaaaata taattaaaag aatgttagat agtgtaccag
    55021 atacttttga taaaagggaa ggttctataa tatataatgc tcttgctcct gttgctatag
    55081 aacttacaga aacatacatt gccatggatg aattactaga ccaaactttt gtagatactg
    55141 ctagttatta ctatttagag aagagatgta aagaaagagg tattacacct ttacctgcaa
    55201 ccaatacaat tgcaaaagga gtttttaata tagatattcc tattgattca agattcaact
    55261 taggagaata caattatgtg gcaattgaga gaatatctga aaaaacatat aaaatgaaat
    55321 gtgaaactac tggacctgta tttgaacttg gtcagttgat tccaattgaa tatgtagaca
    55381 aattagaaac tgctgagtta actgaaatac taattaatgg agaagatgaa gaaagtgaag
    55441 atagtttaag acaaagatat tatgatagcc taaactcaca gagttttggt ggaaatatgc
    55501 aaaattataa agatgaagtt aacaaaatac aagatgttgg aggagttaag gtttatcctg
    55561 tgtgggacgg tggaggtact gttaagttag taataattaa ttctaacttc aaagttccat
    55621 ctagtgattt agttaattta gttcaagaag aaattgatcc tctacaaaac caaggagaag
    55681 gtcttggatt agcaccaatt ggacaccgag tcacagttga aggagttaca agtacaacta
    55741 taaatatatc agcagagata acatacaaaa atggctacac ttgggagaat ataaaatcaa
    55801 ttgcagaaga agcaatagac gactatttaa atgaacttaa catgagttgg gaagatgaag
    55861 aaaacttaat agtccgtata tctcaaattg aaactagatt acttagtatt gatggagtat
    55921 tagacattgc aaatacaatg ataaatgagg ttaaatctaa tctaacaata gatagtaaca
    55981 gtatagtagt gagaggtgag gtagttggat aaagagatta atctaataaa ttacttacca
    56041 caaattctac aagataaaga agaatatata aaagtattta atgtagaaaa caaagaaata
    56101 aaaacactac atgaaaaatt aaatgaccta tcaagtgacc agtttttaga ggatttaact
    56161 ccaagtggta taaaaagatg ggaaaagata atgtctataa ctcctaaaag taatgagagt
    56221 ttagaagata gaaggtttag gatttttagt aaatatataa gtaaactacc ttactcagag
    56281 agatttttaa ggaactggct agatagtata gttggagaag gcaattatga attaactatt
    56341 aataatgcta cttataatat acatcttgaa agtgatgcta gaaatcaaga ttggtttgag
    56401 gaggttcatt cttttgtaag tagtattaaa ccttgcaaca tgactttaga ttacactaga
    56461 gtgcttgtaa gtaaagacaa ttatatgaat tttggtataa caaccctaat aggtcaagaa
    56521 ataactatat acccttggag tccaccagat atagaaactt atggagaaat tgatgtatta
    56581 actggcaatg gagttggata ccaagagata acaatatttt aggaggtgat atattggcta
    56641 tagataaaag ttattacact ataattacag atgtagggaa agcaaagata gcaaatgcaa
    56701 gtgtcacagg taataaagtg ggatttgtaa aaattcaact tggtgatgga ggagggagtg
    56761 aatatactcc aactgagagt cagacagctc tcaaaaacgt ggtatgggaa ggcaatattg
    56821 gaaatacaac tacagatgaa actgcaccaa attgtataat attagagagt ttaataccat
    56881 caagtgtagg cgggtttatg ataagagaaa taggatattt agatgatgaa aataatttaa
    56941 ttgccatttc taaatacaaa gagtgttata aaccttctat agaacaaggt gcagtggtag
    57001 acatgaaggt taaaactgtg cttattgtat ctaatgtaaa taatatagaa cttaaaattg
    57061 acccaacaat aatctttgca acactcaaag atatacaaga cttagaaact aaaataggta
    57121 ctgttaatac taaaattgat acaactaaaa cagaattaac aagcaacata gaaactgcta
    57181 aaacagagtt aaatactaaa atcgaccaat taattgcagg tggttcaaat gttgcataca
    57241 ctcaaagggt cgcaattgat gattgggtgg aggatgcaga aaatggattc aaagcaactg
    57301 taacacatag tttgttaaca cagagaatag ttgtaaatat tatagatgct actacaaaag
    57361 aaaatgtagt tacaaacttt aaaattatag atgataattc tatagaaatt agaagtgaaa
    57421 caaggtcaga attaaacgtt tatgtgataa atggaaatgc agaaactcat tttattaatg
    57481 caactgtaga tgataacagg gtgagtgaaa tgactactta ttcgtctaaa aagatacatg
    57541 aagaaattaa taaggtagca gagcaattaa caggaattaa cagtaatatt atatcaacag
    57601 taaataatga catattacct atatagaaag gagagtgagg aaatggcaac atcaatgaat
    57661 aactttaatt tacctgatat gagaaaatat actaaaaata ttgcgtttga cccatttgca
    57721 ggggggaggc aaagttttga ttggggtggt aataatcatg gtttgcaagg tagctctaaa
    57781 gacacttgtt taagttgtac ttacaatggt tcaacaatta attggggctc aggaaatgtt
    57841 tgggttttgg gaggatatgg tcaatataca tttacttata attgtgaatc aatgcatgtg
    57901 gatacacaac aaaaatttcc atatacttct aatagaataa taactataaa aggtagacca
    57961 gtaatatctg gttcagatac ttcacttggg aataaaagaa aaggatttag tgtagatttt
    58021 actgtaagtg atgatactcc agatgttaat ttgatagtgc gtgtttatct tgatgataaa
    58081 ttaatacaaa acattactcc tgttgtacaa aattcaactt taacagcaac agtaacagat
    58141 agtcaactaa actctttatc catagatgga aatcacaaac taaaaataca attaaatgat
    58201 ggatatgata attttgatag aatttttact tttaaaaaaa tagaaaaggg aattaatata
    58261 tcaacgtcat tagtgactga tagtcaagct aagtttactg taacaaaaac atacagtgaa
    58321 ttgacaaaaa tagaatggta tttagatgaa gtattgaaag atacttttac tacagacttg
    58381 tattcagaaa aaactattaa ctatgaactt acagataatg caatacacac attaagaata
    58441 gttgctacag atgcagaaaa tatagtagag gaaaaagtaa taagtataag taagaatata
    58501 atgccattac aacctaatgc tagtttaagt gatatatcaa ctaagttaac agagattggg
    58561 caaggagtta gaaatggtaa aacaagtatt ataaatactt tagcattaaa gaatatagaa
    58621 gcaagtttga ataacacact tgttgagtta tcagagaaaa ttaaaacgag ttttgatagt
    58681 tcagacgcta gtgttgagga tttaacaaac aaattgacac aagctaataa tactatatca
    58741 cagttaaata caaaatataa atttgcatat agtttggatt tgacagttca ctggtttatt
    58801 gattgtggat ttaagcctga ttttgttatt tatgaatgta taaataaaca tggtaaattt
    58861 cttgttttct taactccttt taattattct gtatttgata ttggaaattt aggttggaca
    58921 attacagcaa gagaagatat aagcaagaaa gattcttttt ctatgaatga cataaatcaa
    58981 ttttggggaa caaagattag agatacagga tttaataatg cagatatgcc aaaagatagt
    59041 ttaaatgata gatatgtttt ttggatagca attaaatgtt tataggaggt ttaatatatg
    59101 aaaaatatag gaagaatagt tttttatgat aatgatacag gggatatatt aatgttttat
    59161 ggagaaaaaa cagtaagtga cagtatagat attaatatcc ctaagataga ttctattgag
    59221 ttgccatatg gaagtataga ttatactaga aataggctaa taggtataaa tgtagaaaca
    59281 aaagaaccaa ttctggaaga aataccagta tatgtaagtg aagaagaaaa aagaatacaa
    59341 gaattagaaa atcaaatatt gattgcagaa aacgaaaaag taggaggatt attataatga
    59401 atataaataa tgttgtagta agaatattag cagagaggat tttgaataaa ggattaaatc
    59461 ccttaaaaaa tagagagttt caacttgatg atgtaactaa tgcagagtac agaaaagcag
    59521 tagaggatta tattatagag catagtggag tagtagaagg agcagaacca acaaaatagg
    59581 ttcttttttt atttcaatta attaggaggc ttacatgaat gaagaactgt tcgaagcaga
    59641 tttaaaaaga catgaaacaa gaataaataa gcatggagaa gaaatagacg aattaaagat
    59701 agcaaatata gagtctaaag cagaattaaa agcattgtgt gagaatctaa actcacttac
    59761 aagtatgctc aaatggctaa ttggaacaat gattacaaca cttataggtt tctttatatt
    59821 tgcaatacaa agaggaatat tttaattaat taggaggtta aaagatggat aatttaataa
    59881 gttttatacc agagcagttg ctaattttag tagctgctct ttatgttata ggagcaggtt
    59941 gcaaaaaata taaacaacta gataataagt atattccagt agtgttatta gcacttggaa
    60001 taggattttc aatatggatg ttaggactaa atgctgattc agtgctgcaa ggtgtaattt
    60061 gttggggagt tgcaataggt ataaatcaaa cttacaaaca attaaaggag gaaaataaat
    60121 aatgaaaata ggtgtaaatt gtggacatac aaagacaggg gcaggaagtg gagctatagg
    60181 gaaaataaat gaatcaatag aaactagaaa tgtaggatat aaagtaatag ataaattaaa
    60241 aactctaggt aacaatgtag ttgattgtac tatagataaa gcatctacac aaagtgagtg
    60301 tttgtctaaa atagcaacac aagcaaatag acaagattta gattggttta taagcataca
    60361 ttttaatgca ggaaaaggac gaggatgcga agtttacaca tacaaaggca aacagtatca
    60421 agatgctata gacgtttgta aaaaaatctc tgatttagga tttacaaatc gaggggtaaa
    60481 agatggaagt ggattatatg tagtaaagaa aacaaaagct aaaagtatgc taatagaagt
    60541 atgctttgtg gacagtgaag atgcaaataa gtatttgagt ttaggagctg ataaattagc
    60601 tactgcaata gtagaagcta taactaaaca tataagttca gcagaagaaa acaattataa
    60661 tagatataaa catacaatag tgtacagtgg tgatgataaa gtatcggcag acattttagg
    60721 attatattat aagagagaaa aagaaagtta tttagttaca gatataaaag actataaacc
    60781 acatagaaca caaaatctat atgtaattgg tggagtaact tgcaataaga tgaaagaact
    60841 gagtaagact acaggagaaa aatttacaga gatttattct gatgatgtgt ggtcaacaat
    60901 ggataaggct agagagtttg taaaaggaaa attataaaac atataatcta ataaaataga
    60961 tataaagata agaagcgttt atataaggtt cttcttatct tttttaataa attgaatttt
    61021 atgccaaacc acattcacat aataaaatca ttattctatg aaggcaatat tctacaatat
    61081 cttctccata ttttttaata aaatcatcaa tttgagtttc tataaatgaa taatattctt
    61141 ttaaactagt aatatatgca gttattatat gaacacattc atcaatcata tctttagaca
    61201 caatcaatcc ccctaaaaaa cgaaaaataa agaatattca gtaaattatg catattacat
    61261 tatctaaaat cttatctaaa caatacttca aaatttaata ttttacaagt agttatgcaa
    61321 aactaggcat tataattgta aaactggtta tatttattaa ttgcataact aaagaaaagt
    61381 acataataat aagaatattg ataggtttga taccttttac aaggtattgg attttataga
    61441 taaataggtt cataatctaa aattaagatg tttatttaca ctttacttac atttttaata
    61501 taaagagaac ttaaatatat gatataacta aggtagttgt acttatgtta atttgatttt
    61561 aaatttacaa gattatttaa atggagaata taattttata gaaatattgg atatatatca
    61621 actcgaaaat aaaattatgc tcagattggt aaaatatgag cttacaaaat acaataaaat
    61681 aatacaacat tagataaaaa gatggtaggt taattctagc atctttctaa tttccaaaca
    61741 taatttttta ttagttaaaa aactataata catattgtaa taataaaacg aacgaaaatt
    61801 atcatactaa aacataagac atgctataat tatattgcat atttc
//
LOCUS       DAEMIK010000002        58244 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEMIK010000002 DAEMIK010000000
VERSION     DAEMIK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.002"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            151..663
                     /locus_tag="KPK87_000086"
     CDS             151..663
                     /locus_tag="KPK87_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG2536290.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSIKSAALSYYSDTNKMPATTSNPVDLENLKTYMESLPDKADIGGEYQLLLVG
                     NKLVLQINDATLTGAQSTKLLSDLGNDKIYKTIGSDDKLTDLLTTNEKLDNKVLYLVL
                     IDNAEMDSTK"
     gene            815..2491
                     /locus_tag="KPK87_000087"
     CDS             815..2491
                     /locus_tag="KPK87_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG2536291.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            2503..3711
                     /locus_tag="KPK87_000088"
     CDS             2503..3711
                     /locus_tag="KPK87_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG2536292.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            3726..5438
                     /locus_tag="KPK87_000089"
     CDS             3726..5438
                     /locus_tag="KPK87_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG2536293.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            5431..6339
                     /gene="pilO"
                     /locus_tag="KPK87_000090"
     CDS             5431..6339
                     /gene="pilO"
                     /locus_tag="KPK87_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG2536294.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            6366..6932
                     /locus_tag="KPK87_000091"
     CDS             6366..6932
                     /locus_tag="KPK87_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2536295.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            6969..7496
                     /locus_tag="KPK87_000092"
     CDS             6969..7496
                     /locus_tag="KPK87_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2536296.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            7517..9055
                     /locus_tag="KPK87_000093"
     CDS             7517..9055
                     /locus_tag="KPK87_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536297.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSINKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILEEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPDQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIINFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            9155..10237
                     /locus_tag="KPK87_000094"
     CDS             9155..10237
                     /locus_tag="KPK87_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG2536298.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            10218..11000
                     /locus_tag="KPK87_000095"
     CDS             10218..11000
                     /locus_tag="KPK87_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG2536299.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            11102..11752
                     /locus_tag="KPK87_000096"
     CDS             11102..11752
                     /locus_tag="KPK87_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG2536300.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            11766..12326
                     /locus_tag="KPK87_000097"
     CDS             11766..12326
                     /locus_tag="KPK87_000097"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG2536301.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            12343..15729
                     /gene="mfd"
                     /locus_tag="KPK87_000098"
     CDS             12343..15729
                     /gene="mfd"
                     /locus_tag="KPK87_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG2536302.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            15760..16755
                     /locus_tag="KPK87_000099"
     CDS             15760..16755
                     /locus_tag="KPK87_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2536303.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            16879..17418
                     /gene="spoVT"
                     /locus_tag="KPK87_000100"
     CDS             16879..17418
                     /gene="spoVT"
                     /locus_tag="KPK87_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG2536304.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            17538..19151
                     /locus_tag="KPK87_000101"
     CDS             17538..19151
                     /locus_tag="KPK87_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2536305.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            19258..20706
                     /gene="mazG"
                     /locus_tag="KPK87_000102"
     CDS             19258..20706
                     /gene="mazG"
                     /locus_tag="KPK87_000102"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG2536306.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            20795..21073
                     /locus_tag="KPK87_000103"
     CDS             20795..21073
                     /locus_tag="KPK87_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG2536307.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            21145..21387
                     /locus_tag="KPK87_000104"
     CDS             21145..21387
                     /locus_tag="KPK87_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG2536308.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            21449..21703
                     /locus_tag="KPK87_000105"
     CDS             21449..21703
                     /locus_tag="KPK87_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG2536309.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            21704..22237
                     /locus_tag="KPK87_000106"
     CDS             21704..22237
                     /locus_tag="KPK87_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536310.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            22243..22527
                     /locus_tag="KPK87_000107"
     CDS             22243..22527
                     /locus_tag="KPK87_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG2536311.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            22640..23560
                     /locus_tag="KPK87_000108"
     CDS             22640..23560
                     /locus_tag="KPK87_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG2536312.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            23650..26022
                     /gene="spoIIE"
                     /locus_tag="KPK87_000109"
     CDS             23650..26022
                     /gene="spoIIE"
                     /locus_tag="KPK87_000109"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG2536313.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            26373..28265
                     /gene="pepF"
                     /locus_tag="KPK87_000110"
     CDS             26373..28265
                     /gene="pepF"
                     /locus_tag="KPK87_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG2536314.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            28379..29344
                     /gene="galU"
                     /locus_tag="KPK87_000111"
     CDS             28379..29344
                     /gene="galU"
                     /locus_tag="KPK87_000111"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG2536315.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            29844..31448
                     /gene="rho"
                     /locus_tag="KPK87_000112"
     CDS             29844..31448
                     /gene="rho"
                     /locus_tag="KPK87_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG2536316.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            31601..31801
                     /gene="rpmE"
                     /locus_tag="KPK87_000113"
     CDS             31601..31801
                     /gene="rpmE"
                     /locus_tag="KPK87_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG2536317.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            31946..32839
                     /locus_tag="KPK87_000114"
     CDS             31946..32839
                     /locus_tag="KPK87_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG2536318.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            32857..33705
                     /gene="prmC"
                     /locus_tag="KPK87_000115"
     CDS             32857..33705
                     /gene="prmC"
                     /locus_tag="KPK87_000115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG2536319.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            33749..34813
                     /gene="prfA"
                     /locus_tag="KPK87_000116"
     CDS             33749..34813
                     /gene="prfA"
                     /locus_tag="KPK87_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG2536320.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            34942..35622
                     /locus_tag="KPK87_000117"
     CDS             34942..35622
                     /locus_tag="KPK87_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG2536321.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            35651..36691
                     /locus_tag="KPK87_000118"
     CDS             35651..36691
                     /locus_tag="KPK87_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG2536322.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVASKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            36708..37157
                     /locus_tag="KPK87_000119"
     CDS             36708..37157
                     /locus_tag="KPK87_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG2536323.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            37174..37626
                     /gene="rpiB"
                     /locus_tag="KPK87_000120"
     CDS             37174..37626
                     /gene="rpiB"
                     /locus_tag="KPK87_000120"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG2536324.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            37662..38291
                     /gene="upp"
                     /locus_tag="KPK87_000121"
     CDS             37662..38291
                     /gene="upp"
                     /locus_tag="KPK87_000121"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG2536325.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            38592..39407
                     /locus_tag="KPK87_000122"
     CDS             38592..39407
                     /locus_tag="KPK87_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG2536326.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            39633..40070
                     /locus_tag="KPK87_000123"
     CDS             39633..40070
                     /locus_tag="KPK87_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG2536327.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            40255..40482
                     /locus_tag="KPK87_000124"
     CDS             40255..40482
                     /locus_tag="KPK87_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG2536328.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            40487..40873
                     /locus_tag="KPK87_000125"
     CDS             40487..40873
                     /locus_tag="KPK87_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG2536329.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            40876..41580
                     /gene="atpB"
                     /locus_tag="KPK87_000126"
     CDS             40876..41580
                     /gene="atpB"
                     /locus_tag="KPK87_000126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG2536330.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            41649..41909
                     /gene="atpE"
                     /locus_tag="KPK87_000127"
     CDS             41649..41909
                     /gene="atpE"
                     /locus_tag="KPK87_000127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG2536331.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            42032..42529
                     /gene="atpF"
                     /locus_tag="KPK87_000128"
     CDS             42032..42529
                     /gene="atpF"
                     /locus_tag="KPK87_000128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG2536332.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            42526..43071
                     /locus_tag="KPK87_000129"
     CDS             42526..43071
                     /locus_tag="KPK87_000129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG2536333.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            43088..44590
                     /locus_tag="KPK87_000130"
     CDS             43088..44590
                     /locus_tag="KPK87_000130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG2536334.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            44618..45496
                     /gene="atpG"
                     /locus_tag="KPK87_000131"
     CDS             44618..45496
                     /gene="atpG"
                     /locus_tag="KPK87_000131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG2536335.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            45509..46903
                     /gene="atpD"
                     /locus_tag="KPK87_000132"
     CDS             45509..46903
                     /gene="atpD"
                     /locus_tag="KPK87_000132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG2536336.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            46906..47166
                     /gene="atpC"
                     /locus_tag="KPK87_000133"
     CDS             46906..47166
                     /gene="atpC"
                     /locus_tag="KPK87_000133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG2536337.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            47628..48008
                     /locus_tag="KPK87_000134"
     CDS             47628..48008
                     /locus_tag="KPK87_000134"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG2536338.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            48023..48484
                     /locus_tag="KPK87_000135"
     CDS             48023..48484
                     /locus_tag="KPK87_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG2536339.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            48505..49095
                     /locus_tag="KPK87_000136"
     CDS             48505..49095
                     /locus_tag="KPK87_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536340.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            49110..50267
                     /gene="alr"
                     /locus_tag="KPK87_000137"
     CDS             49110..50267
                     /gene="alr"
                     /locus_tag="KPK87_000137"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG2536341.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            50300..50587
                     /locus_tag="KPK87_000138"
     CDS             50300..50587
                     /locus_tag="KPK87_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG2536342.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            50556..50939
                     /locus_tag="KPK87_000139"
     CDS             50556..50939
                     /locus_tag="KPK87_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG2536343.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            51110..51937
                     /locus_tag="KPK87_000140"
     CDS             51110..51937
                     /locus_tag="KPK87_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG2536344.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEEMNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGVDNE"
     gene            51930..52871
                     /locus_tag="KPK87_000141"
     CDS             51930..52871
                     /locus_tag="KPK87_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG2536345.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKTF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            53294..54388
                     /locus_tag="KPK87_000142"
     CDS             53294..54388
                     /locus_tag="KPK87_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536346.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDRLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPIIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDNHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            54613..55029
                     /locus_tag="KPK87_000143"
     CDS             54613..55029
                     /locus_tag="KPK87_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536347.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(55211..55780)
                     /locus_tag="KPK87_000144"
     CDS             complement(55211..55780)
                     /locus_tag="KPK87_000144"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG2536348.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGEYGIR
                     EPNEKSPTVDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            55934..57091
                     /locus_tag="KPK87_000145"
     CDS             55934..57091
                     /locus_tag="KPK87_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG2536349.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            57695..58177
                     /locus_tag="KPK87_000146"
     CDS             57695..58177
                     /locus_tag="KPK87_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2536350.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
BASE COUNT        23457 a         6211 c        10782 g        17794 t
ORIGIN      
        1 aaatactatt attttcatag ctttttttat ttaaaaaatg gttatatgtt agaaataaat
       61 ttggttagta cgtttaaata attttactta gatttttaac cagatatatt ataagtaagt
      121 attaattaaa aaatttaagg gggaatgaaa atgaagttaa aaaagaataa aaaaggtttc
      181 actttagtgg aattattggt agtaattgca attataggta tattagcagt agtggcagtt
      241 ccagctttat ttagtaatat aaataaggct aaggtagcaa gtgttgagtc tgattatagt
      301 tcaattaaga gtgcagcatt atcttattat tcagatacta ataagatgcc agctacaaca
      361 tcaaatcctg tagatttaga aaatttaaaa acttatatgg aaagtcttcc tgataaagca
      421 gatataggtg gagagtatca attacttttg gttgggaata agttagtttt acaaataaat
      481 gatgctacat taacaggagc gcaatcaacg aagttattga gtgatttagg taatgataag
      541 atatacaaaa ctataggtag cgatgataag cttacagatt tattaactac caatgaaaaa
      601 ttagataata aggttctata tttagttctt atagataatg ctgagatgga ttcaacaaaa
      661 taattaaaac tatattttta taaagaatat tgtaaattta atgaaaaata taatataaaa
      721 gacaggaaga aaatcttcct gtttttttat actattttct ttaatgttca attatgaatg
      781 atgatataat tgagatagta aaggagtgaa acctgtggct aaaaaagtta gaataggaga
      841 caaactggtc gaaaaaggat atataacaga agaacagctt aaatgggcac tatcagaaca
      901 aaaaaacagt ggaaaaagac taggagaatt tctagtacaa gaagggctta tagatagtaa
      961 cttactaata agtgtattaa aagaactctt ggatatagaa agtatatttt tagaagggac
     1021 agaaatagac actttagcta caaagatggt tccagaaaat atatgcaaaa gatatactgt
     1081 atttccattc aaaatagatg gaaataaaat atgtctagcc atgtcagacc cacaagacag
     1141 agaagcagtt caagatgtaa ggcgtatgtc aggtaaagat gtagagatat ttatatcaag
     1201 tacagaagat ataaacaaag caataggtca tgcttatgca cattcagaaa taaataaggc
     1261 aatgactgag tacaacaaaa atagaactgg tggagtaaga gaaacagtaa tacttgaaga
     1321 agatgtaaat gctgctccaa tagtacgatt agtaaataac atactagaaa atgctgttag
     1381 aatggaagca agtgatatac atatagaaca aagtgaaaat tacatgcgtg ttaggtttag
     1441 gatagatgga atgttaagag aatatatgag gatgaactca gcaccatata aggcagtaat
     1501 cagccgtatc aagataatgt cagatataaa tatatctgaa aagagaatac cacaggatgg
     1561 tagaatctat ttaaaagtag ataataaacc catagatttt agggtttcta caatgccaac
     1621 taacagggat gaaaagatag ctatgagggt tcttgataag agtaatttta tggttagtaa
     1681 agaagtacta ggaatagacg aacatggctc taagatatat gatgaattga taaacactcc
     1741 ttatggtctt atattggttg ttggccctac tggaagtggt aaaaccacta cattgtattc
     1801 aatgttaaat caactaaata cagaaaatag aaatctatta acaatagaag accctataga
     1861 gtatgaatta cctggtgtaa atcaatctca aataaatgaa aaggcagggc ttactttcgc
     1921 cagcggactt agagctttta tgcgtcaaga cccagatata ataatggttg gagagataag
     1981 agatactgag acagcagaaa tagcaattag agcttctctt acaggacatt tagttttaag
     2041 tacattacat gcaaatacag cagttggagc aatttcacgt cttttagata tggatgtaga
     2101 aagtttttta ataacttcat ctgttttagg tgtaatatct caaagactta cacgaaaaat
     2161 atgtgagcat tgtaaggttt catatgaagc agatattgga gaaaaaaagg ctcttggtat
     2221 tgatgtaaat gaatcagtaa ctatttatag aggaaaaggt tgtgaaaggt gtaataatac
     2281 tggatataaa ggacgtctag gtatatttga aatgcttgaa attactccag aaataaaaga
     2341 gttaatagat tcatctgcaa atcaaagaga aattttaaag atggctagaa aacaaggtat
     2401 ggttagctta aaagaggata tagttaaaaa ggttttaaat ggaaagacaa cagtagaaga
     2461 aatgattaga ataattttga tgactgatta aaggatgata ttatgaaaag ttttaaatac
     2521 acagtaataa aagaggacgg aaaaaagcaa actgatgtga ttgaagccaa cgattttaat
     2581 gaagctagaa atcttcttag aaagagaaat cttagagtta ttgaaatcaa agaaagcaaa
     2641 aataaaaata taagattatc tagtagaaag agaaaaaaag acttaggagc tgaccagata
     2701 agccactttt gtagacagtt tgctattata gtatcttctg gtataaatag tatttctgga
     2761 ttagaaacat tagctagaag gtcaactaac attacattaa gagaagaaat taatcgtata
     2821 gtggcagaaa taaaagtagg ttctactatt gcagattcaa tgctttcatc aaaatcaaag
     2881 tttccaaagt tacttggtgc tatggtagct acaggtgaag ctacaggtaa attagatgaa
     2941 gtattaaaaa gtatggcttc attttatgca agtgaacaca gggttaaaca aaagcttaga
     3001 aatgctgcaa catatccatt aatagttttg atttcttcat ttataatgat ttttatattt
     3061 acaacattta tggtgcctaa gatgattaat tcaattacaa cagtaggagc aactcttcca
     3121 cttataacaa gaattgttat ggggtttggt atgtttatga agaagttttg gataatagta
     3181 cttttagtta ttatattatt tatctatcag tttaaaaaat atctaaaaac gtcagttgga
     3241 agggctcata aagatagagt tataaacaag atacctgttt taggaaaagg aataaattgt
     3301 atggtagcta cacgattttc aagagcattg tatctatttg tatcaacagg atacccatta
     3361 gttcaagggc ttgattatat tatagatagt gtaaataaca ccatggcaga gaagatttta
     3421 gcatcagcta aagatggaat aacaagagga gaaggcttag cagaaaattt agagagatat
     3481 acgtattttg actctgtgtt ggtacagatg atagctatag gggagcagac aggggaatta
     3541 gagaatataa gtagacagat ggcagaattt tatgagtatg aatccgaaat ttatttaaat
     3601 aggatggcct caatgataga gcctgtttta ataattgcag ttggtattat agttgcgata
     3661 ctggttgtat caatatttat gccaatgctt agtatatatg atgccatgta ggagggaaag
     3721 gacaaatgaa caaggtttat gtatcatatt atggcgattc tatttccata gttgagggca
     3781 gatataaaca cgataaggat aagttttatg taaaaaattt taatgtgttt tcaatagagg
     3841 atattgcaca agatttttct aaggaaaagt atgctctatt gagatacgca ttagaaaatg
     3901 ttaagttaaa atcaagacat gtagtttttt gtttaaatac aagagatgta attttaaaac
     3961 cccaaaaact tcctaatatg gacaaaaagg atttagatgg atttatgaag ctagagatgg
     4021 acgaaatgat ggctttggag tcagatgaat atgtattttc gtatgaagta tctagtgaaa
     4081 cacctgataa ttcagatggc gatgaatcaa gcttaaatct tatcattgga gctatacaaa
     4141 aggaagaggt aaatgcaatt gtagagataa tgcatgaatt tgatttagta ttagatagaa
     4201 tagatacatt atcaacagct tacttaagaa tgcttaaaaa tttagactac aatgatataa
     4261 tggtaaccaa tataagtgac aatagcacaa ttataaatat atataagaaa gatactttat
     4321 ttattagtga taatatacct ataaagataa ctgctaaaga tatggacatg caattattaa
     4381 atgtagtaga agaatcaaaa ggtcttatga attactattc gtcaagaaac tttggcaagg
     4441 ttacggatac tatattaata ttaggaaaaa gatattccaa taaattaatt tatgaaactt
     4501 ttagctctgc attttcaagc tatgtttctc aaggccttac tgagataatg gatatatctg
     4561 atatgataac tggagatata gatgaagatg atataaattc aattgtagag cttttaggaa
     4621 gtatgataga acctaaaggg aaaagagatt ttgaaaatat aaacttttta ccaatggata
     4681 tacttagaaa acagataaaa gctaaacaaa tgaaaaacat actgaaaatt gctccaatag
     4741 ttattttgat tcttgctata ccttatttat gtttaatttt ttcaaataat ttagcaaata
     4801 aggaacttga aaatgttaat ggtgaaataa gaaatattga atctgcatat tatcagattg
     4861 gaaatataga aaaaatgata cagagtaaaa gcgaagaaat aaaggtatat gatatgttaa
     4921 ttgggaaaaa ggtaaagtgg gaaaatttat tggatgatat agataaaaat actccaagta
     4981 aagttgaact aacaagttta tcaacaactt atcaaaatct tggaactaat aataaagctg
     5041 ataattcaaa aagtaataag actacaacag ataaatcaag taacaatgaa acaaaatcaa
     5101 gtacgaaaag tgatagtaat ggagataaaa cagataatga tttaaatgaa gataagacta
     5161 caaatgtgtc agaagcatta actggaaata gtgagactaa tagtaataac acagataaag
     5221 atgataaaac tccattgtat gataagatac caaatgttat aaatatagaa ggaaatgcta
     5281 aagattcctc ttatgtaggt caatttttat ataaattaaa gggattatac tatttcaagg
     5341 atgttaaatt acatagcata aaaagtaatg cagaagattc taactataca tttagtatga
     5401 cattatacct gaaagaaggt gtattaacta atgaataaag atatatttaa aaaagatata
     5461 aaagatatat ttaaaatgga agttagtcta aaagggattc ttataaaggt tataatttta
     5521 ttagctattt tatcatatgg ttattttgga ctttaccctc aatatcagaa atatattgct
     5581 acaaaaaatt ctgtaaaaca ggctactgaa aaattaaata tatataaaga gaaaactgca
     5641 cttatgccaa aatatgaaaa acaacttgaa gaagttgaaa gtgaattaaa agaaaaaagc
     5701 aaaaatcttt tatatgatat gcaagatggt ctttttttaa taggtttaga taaaaaaatg
     5761 aaggcttata atattgactt aaccagttat gacgttggaa atgttaaaga atacgaacat
     5821 ttttatggta tatcaacaac aataagtgtt agaggtgatt atagaaatgt caaaaaatta
     5881 atggcttatt tagaagaaca aaaaaatgtt actcaaatac tagattatac aatgacttca
     5941 tatatagaac cagaaaaaga agaggaagaa acaacaaaca ataaggattt aattaaaaat
     6001 gtgaacataa ataaagatac aaaaatatac tgggtggaag gcgaaaataa gtggcattta
     6061 tataaggatt gtccattgat aaaagaggga gagattctaa aaaatgacaa acttttagat
     6121 aggtctacta attatctatg taatacttgt gtacttagag ctgcaacaga aaaagaagca
     6181 aaaaaagaag tcgtagctcc aaaggcagaa ggtgtagtag atgctacatt taaatttata
     6241 atgtacacta aagagaatac tacaatgata cttgatacag ataatccatc ttcatggaga
     6301 ccaggaaaat ataatccatt taagtctagt gcgaaataaa gctaagtaag ctttaaagat
     6361 aggtgatgag tttgtacaaa aataatgaaa aaggtcttac tttattagaa gtaataatag
     6421 ctgtttttat actgacaata gttttaagta tttcttataa agtgttcaat ggtataacat
     6481 cagcagtaaa aaagcaacag attattacag atgctcaagt aaatattaat ctaattaata
     6541 agtatctaaa tagagatttg gaaaactgta aagaactaac taaaactggt tcaggtaata
     6601 attatgaata caatatagag atgccagata atgttgtaaa atatgaagtt tctatagaaa
     6661 ctaaaaaaaa tacagaggta tattctgtaa cgagaatcca aaacaataca attgatacag
     6721 aaaatgaagt tagagaagag ataatctata atcaaccact agttcaaaac aataaagaaa
     6781 tgaaggaaac accatttaaa atagaaaagc aaactggtaa atctatttat actgtaagta
     6841 tatactataa tgaatcagtg caagaaagtc ataaaaatag caatttaaat aacaaaactt
     6901 atacatttga tgttatgtca agaataggat aataagaatt tattaaaaaa taagtaggtt
     6961 gataaaatat gagtaaaaag aatagtgaaa gaggattttc cttaattgaa gtgttagtag
     7021 ctatggctat tatgggaatt gtactatttg cattttttaa tattatcaat actaataaca
     7081 aagcaaacac taaaaatgac acagatataa cttccttaaa ctatgtccaa agtgaaatag
     7141 aaaatctaag agaaaagata aaaagtggag aatttgattt tgatagttta gataaactag
     7201 aagatggaac tgtcgtatat gaaaaattaa tagataaatc aaaaaaagtt gtatatgata
     7261 aagttcttag tgaaggtgat gtgagcttat atgatactcc atatgaaaag attacaacaa
     7321 taaaagatga agatggtaat ttaatcgaca aaggaaatat tacaaataag ataaagacta
     7381 ttgtagaaga taaaagtggt cagatatata aaatagctgt aactgggaaa agcatgaatg
     7441 actattcaag taaaaaagag gttaaaattg taactgaaat atttaaagat aaataatttt
     7501 acttgggggt gagattttga ggaagtggaa taaatttaaa agtgaaagag gggcagcttt
     7561 agttttagtt ttggttgtag tagcattgct ttctattgtt ggattgatat tttcaaatca
     7621 gatagctaat agaataaaat ctacaaagac tactaatgaa ggtatacaag caaagtattt
     7681 agcagagaca tgtgtggaaa atagtattaa taaagcatat gaaaaattgt atgatgagct
     7741 tgagaaaatg gataatgaat ttaaaagtga aaatcaagaa aaatccatat ctaggtcaaa
     7801 attaagaaat atatcagatg aagattttaa taatcaagat gaggaaaata ttgaagcaga
     7861 aagacttgga tatatgaata atataaattt ctatttaaat aaagcttctt cagacttgga
     7921 aaaagctagt atggaattga aaaagctata tgatttagat atgttggact atagagacat
     7981 tgaatatgta gatgcaaata ttatttcaca tagagattct atcttagaaa tttgcaagaa
     8041 ttatacttct ggagatatta gtaaaataaa tgaatatatt ctggaagaag atatagactc
     8101 aactacattg attgaggcaa agttagtgaa taatgatatt cttttaaaaa tgtttttaga
     8161 agaaaataaa attgaaaatg aacatttaaa ttcagccttt tcacatactt ataaggcatt
     8221 agataacatt tctttggcga tgcaaaatat gattgagtat agacatacgt ttcatataga
     8281 tgagccaaaa gtagaagtat ctaatgggat tccagattct caacaatatt atgaactaat
     8341 acaaaatcct ataataaata gtatggagta tatctggaat agtaaatggg atacattaga
     8401 aaacctattg gaaattttac cagatcaaac tcaaggattt aattctttaa gagttcattt
     8461 gagaaataat gtaagaaaat ttgagaagtt aagcgataat atatctagtg gtaaaaaaaa
     8521 tacagcaaaa aatttcttaa agtataaaga gttattgtat gaaatatcag accaatgtaa
     8581 tcaactaaaa agtatgtctt atgagaaaat acctgttaaa tatgataaca tggctttgat
     8641 aacaactttt gactatatac aaaatgagct tttagcagag ataaaatgca gattaaaaga
     8701 attaaaacca caagaaattg ataaaacaga aggtattact ataaaaatac ctttttataa
     8761 ggctgactac gacatgacaa aagaaggatg gcctaaatta aaagaaaatg gttcaggggc
     8821 ggagttaagc ttaatggtaa ctggtgataa agatggaata aaagaagtag aagttacaga
     8881 tggtaaaaaa aatattattg gtttaggtgt tgaagaaaat tcaaattcaa agtataaggt
     8941 tgacgccatt ataaacttta atctaaatat agatacaaat gtagttggaa attatgatat
     9001 aaaagataaa attttgatta atcatgatat aagttcatac aaaaaagtaa actaaatata
     9061 agaagcaact tagtataaat aaataataaa ataattttat tatttactac taaaaaaata
     9121 agtaaactaa tagaagatat ggagagtaaa tattatgtat atttatgatt tattagagca
     9181 aggtataaga ctaaacgcat cagatataca cataaccgtt ggaacaaatc ctgtagctag
     9241 agtaaaaggt ggatttgtaa aactaagtga acaaatattg acatcagaag taacaatgca
     9301 aatggcaaaa gacatagcgg gggaatctat gtttaaagta atagaagaac atggagaagc
     9361 tgatttttca gcttctctaa aaacaggaga aagatttagg gtaaatgcat ataggcaaaa
     9421 aggaaattac gcaatagcaa taaggactat aacagcagaa attccaacat ttgaaaaatt
     9481 aggccttcca gagagtataa aatcttttac tgaaaagcat aaaggattag ttttagttac
     9541 aggtccaaca ggaagtggta aaagtacaac acttgcaagt atgataaata taataaatga
     9601 aaaacaacaa aaacatataa ttacacttga agacccgata gaatatgtac atcatcataa
     9661 acaaagttta gtaaatcaaa gagaagtagg aactgataca gaaagttttc attcagccct
     9721 tagagctatt ttgcgtcagg acccagatgt aatactaata ggagagatga gagaccctga
     9781 aactgtttca atagcattaa cagcagcaga aacaggtcat ttagtatttt caacactaca
     9841 tacagtgggg gcagctaaga caattgatag aatagtagac atgtttcaac catcccaaca
     9901 gcaacaaata aaaacacagt tatctacagt ttgtgaaggt gtggtatcac agcagttact
     9961 tcctacagca gatgggaaag gtagaatagc agcaattgaa ttgatgtttg ctactccagc
    10021 aataaaaaac ctgataaggg aaggcaaaac atatcaaatt ccaaacatga tacaaactgg
    10081 agttaaagta ggtatgaaaa ctatggacca agatttaatg gaactttata aaaatggaaa
    10141 gattacaaaa gatatggcac ttagtaggtg tactgaccaa gagtttatga caagaatgat
    10201 aggtggagta aactacaatg gatattataa tcgttaatct atatgtattt atagtgggta
    10261 tagtgtttgg aagtttcttt aacgtctgta tttttaggat tccagaaggt atatctattt
    10321 ctagaccacc ttctcattgt atgagttgta atactagact aaagccaata gacttaatac
    10381 cagtgctaag ctatttaatg agtggcagaa agtgtagata ttgttatgag aaaatttcta
    10441 gtagatatgc tatagtggaa ttaattacag gacttttatt tttaacagta tttatgttat
    10501 atggaatatc tacatcaact atttattatt tggttcttgt gtctttactg attataatta
    10561 catttataga tattgaccac tttataatac cagataaaat attggttttt ggagctatat
    10621 tttctatgat ttttaacctt ttatttaaag aaattccagt taaggattca attctaggtg
    10681 tttttatttg tggaggaagt gtctggatta tagttctttt gattgaattt gtaataaaaa
    10741 aagagtgtat gggtggagga gatatcaaac tatttgcaat gctaggactt tacatgggtg
    10801 ttaaaaatgg tcttttgaca gccttactaa gcgtttatgt aggagcagtc tatggaatct
    10861 gtgtaataat tataagtaga ataaaaggaa aggaatataa ttctgttata ccatatggac
    10921 cttttatatc tattggagca ttaataacaa tcttatctgg aaagcaatta cttgaacttt
    10981 atttttctat ggttatatag atgatagtca agttaaataa agttttaatt taaaaaaatg
    11041 aattaaagta tttaataagg acaatattat tattaaccct attactaaag tgctacaaac
    11101 tatggagtat ataataataa tttccttatt ttttgcacat ttgataaata aatctctatg
    11161 tgttttttgt gatagagaaa agaaaaattt atataatacg ttttttattt acataataac
    11221 tgtagtttta tctgtattaa tatatctaaa atttgggatt agtgtagaat gtgtacaata
    11281 tatggtttta attccatttt taataattat atccattgta gattactaca caatatatgt
    11341 atatgatata acaatagtta gtggtataat agttcaaggt ataatttttt tggccttttt
    11401 caatatagct caatttgatt atataaatca tatatttgga ctattgatag gctttattat
    11461 accttatatt attgctaaac taacaaaagg attaggctat ggagatgtag gtctctatag
    11521 cttatgttgc tttgcattgg gaaataatta tgggttttat gtgatagttt tatcatttat
    11581 tttagcgttt ttgtttttta ttattttata cataactaaa atatttaaag atgtagatat
    11641 aagaaagcaa attcctttta caccttttat ttccttgtca acaattttaa taatatttac
    11701 agagtataat atatttaatt tttattataa tttgttatac aaatttatat aacaggaggt
    11761 aagaaatgta cgtagtagta gggttaggta atccaggaaa gaagtatgag aagactagac
    11821 ataacgttgg atttgatgtt atagatattc ttgctaaaga atacaatatt agtgttacta
    11881 aaataaagca taaagctttg attggtgaag ggagagtagg tactgaaaaa gtattattag
    11941 ttaaaccaca aacttatatg aatttaagtg gagaaacatt aatagatata tataaatatt
    12001 ataaggttga tttaagtaat atagtagtag tatatgatga tatagattta gaagttggaa
    12061 agatacgtat aagaaaaaaa ggaagtggag gaactcataa tggaatgaaa tctataacta
    12121 agtgcctagg ttcaaatgac tttccaagag tgagagtagg tgtatctaaa ccagaagctg
    12181 gtcaagactt agcagacttt gtactttcta gatttagaaa agaagaatct gataacataa
    12241 atgaagcatt agaaaaagcg gcagatgcta ttgactcaat aataagagaa aatatagata
    12301 tgtctatgaa caaatataat ggatagtgag agggagattt aaatgaatga tgttttttta
    12361 tatcctttgc aaaattcaaa agagtataaa gatataataa actgtataaa aaatacaaaa
    12421 ggctcattat tggttaatgg acttttacca gtacaaaaac ctcatatatc gtattctata
    12481 tttaatgatt tatcacgaca aatgattttt ataacgagta gtgatttaga agcaaagaaa
    12541 gtatacgaag atttaagttt ttatatggaa gataaagttg aatatttagg ttttcaagat
    12601 atttattttt atcacttgga tgcaaaggac agaaacgaag aagcaaagaa attaaaagta
    12661 ttgttaaaac tagcaaataa agaaaaaatc attttagtaa cttctattga ggcagtttta
    12721 agaaaataca taccaaaaca agttttgctc gatagtgtat ctcattataa ggtgggagat
    12781 agcttagact tagaaaagtt aactgagaaa ttagtttcat tgggatatga aagagtttca
    12841 aaaatagagg ggtttggtca atttagtata agaggtggaa ttattgatgt attttcactt
    12901 gaatatacaa atccaattcg tatggaatta tttgatgatg agatagattc catacgaact
    12961 tttgatgtat attcacaaaa atcaattgat aaattgcaac aattctctat aactccatct
    13021 agagaattta tttatcccga aaaaactaca gatgcattag ttaaattaaa aaaagaaaca
    13081 actaaaaata ctgatgaagg tacatttcaa aatatagatt atatatcaag taagacttat
    13141 tttgaaggtg cagagaacta tatagattat atttatccag aagaaaacaa aagtatattt
    13201 acttatttag cagatgatgc aatagtattt atcaatgata taacgagact taaagaaaga
    13261 tgcgaaaatt atataaatga gtttagagaa aattataaat taaatcttga aagaggtctg
    13321 gctattaaaa accaaggtaa attgttatat cactatacag atttagagta cttagtaaaa
    13381 gacaagtctg tggtattaaa ctcgctattg ccaaagtcta taaataactt ttctataaaa
    13441 tctataataa attttgaaag tagagaagtt cctacattta atggaaaggt agatttgctt
    13501 gtagaagaat taaatcggct gaaatataat ggatacaaaa taattttagc aacaaatacc
    13561 ttagaaagag caaataaatt aggaaaagat ttattggaca aaggcttaga aacgaccatt
    13621 tctaaagata gagatattga aattaaatct tcacaagtta taattgtacc tgcacacata
    13681 aacagtggtt ttcagtataa atctataaag tttgttgtta taacagataa cgaaatgata
    13741 ggtgtttata aaagagcgtc taagactagc aataaaaaag ttaaaaaggg aaagaaaata
    13801 gaatcctttt tagatttaag tgttggagac tatgtagtcc atgaaaatag tggtgtaggt
    13861 agatacacag gaattgaaca aattacagtc aatgctatta aaaaagatta tatgaaaata
    13921 gtatatcaag gtggagataa tctatatgtg ccaatagacc agatggacaa agtacaaaaa
    13981 tatattggtg cagaagtaga aaaagttaag ttaaataagc ttggaacaaa tgagtggacg
    14041 aaagcaaaag caaaagttaa aaaagaaatt gaagatatga caaaagactt gattgagctt
    14101 tatgcaaaac gtgaaaaaat acaagggtat aagttttcaa aagatacacc ttggcaggca
    14161 gagtttgaat cgttgtttcc atatcaagaa acagaagacc aattaaaggc tatagaagaa
    14221 actaaaaaag atatggaatc aagtaaggta atggataggc tagtatgtgg agatgttggt
    14281 tatggaaaaa cagaggtagc tattagaagt atttttaaag cttgtatgga ccaaaaacaa
    14341 gttgctgtat tggttccaac aacaatattg gcccaacaac attataatac attcaaagag
    14401 aggtttgaaa attatccttt gagagtagaa gttttaagta ggtttaaaac accaaaacaa
    14461 caaaaacaga ttattgaaga tgctaaaaaa ggattagtag atattttaat tggaacacat
    14521 agaataatat ccaaagatat aaatttacca aacttaggtc ttgtagtcat tgatgaagaa
    14581 caaagatttg gagtaaaaca taaagaagct ttaaagaaaa ttaaatctac agtagatgta
    14641 cttacacttt cagcaactcc tataccaaga acattacata tgtctttaag tggaataaga
    14701 gacatgagtg ttatagaaga acctccacaa gaaagacatc ctgtaataac atatgttaca
    14761 gagagtaagg aaagcgtaat tcaagatgag atagagagag aattaagtag aggtggacag
    14821 gtattttttg tatacaatag agttgaacac attgaagaaa tggcaagtat gattcaaaaa
    14881 ctcgtaccag atgctagagt ggctgttgca catggaagaa tgacttctaa atcattagaa
    14941 aatataatcc taggattttt aaataaagat tatgatgtac tagtctgtac tacaataatt
    15001 gaaactggta tggacatatc aaatgcaaat acaatgatta tatatgatgc agataaaatg
    15061 ggattggctc aactttacca attgagaggt agggttggaa gaagttcaag acaaggatat
    15121 gcatatcttt tgtatgaaaa agataaaact ttaagtgaga tagcggagaa gagattaaaa
    15181 gctatcagag aatttacaga gtttggttct ggttttaaaa tagcaatgag agaccttgaa
    15241 ataagaggag caggaaatat tttaggttct caacaacatg gtcatatggc agttataggt
    15301 tatgatttgt acgttaaaat gcttaatgat gcaattaaaa aagtcaaagg tgaacctatt
    15361 gtggaggaaa ttgatgttga aatagattta agtgtaaatg catacattcc agataattat
    15421 atcaaagatg agttgattaa aattgaaatg tataagaaaa tagcttctat tgaaaacaaa
    15481 gaagacatgt tggatataca agaagaactt gaggatagat tctctgatat acctaaacca
    15541 gtccagacac ttcttacaat agcttatata aaatcgttat gtaagatatt aaaaatagaa
    15601 aagataagac aattaaaaga tgaaatatta ttagttccaa taacaaagta tagaactaaa
    15661 cagaaaatag gttataatat agttacagaa ttagaagaat tattagaaaa gatgtgcaag
    15721 gtaaaataac ttgtaaattt aagaagggat gttttaaatt tgaaaaaagt aataacattg
    15781 gtaatagcta tgattttggt agtatctgtg actgcttgca gttctagtaa aggagaaact
    15841 gtggcaacag ttgaaggtac aaagatatca tcagatgaat ttaaaaaaac tatagctctt
    15901 tataaagatt ctatggaaca aacttatgga aaagatatat gggataagga agttgaaaag
    15961 ggtgtaaaat acaaggacaa gtttaaagat ttaattcttg accaacttat aactacagaa
    16021 gtaatatatt ctcaagctaa aaaagataac ttacttccta aaaaagagga tgttgaaaaa
    16081 agttttaaag aattaaaaga cgctatgggt aaagatgaaa aatataaaga acaacttaag
    16141 aaattaggta tagatgatga gtttttaaga gaccaacaag agaaagattt agcaatgcaa
    16201 aactatcaaa gtaactttgc taaaaagact aaaatatcag atgaagaaat gaaaaaatac
    16261 tatgatacac ataaagatga atttaaaaaa gatgaggtag aagcttctca tattttattg
    16321 aaaacagtag atgataataa caagccactt tcagataagg aaaaagctga agctaagaaa
    16381 aaggctgaag aggctcttaa agaagttaaa tcaggtgagg attttgcaaa agtagcaaaa
    16441 aaatattcac aagatacttc agcaagtgat ggtggtaaat taggattctt ttcaaggggt
    16501 caaatggttg ctgaatttga agatgctgct ttttctatga aaaagggtga agtatctgat
    16561 ttagttgaaa ctcaatatgg ataccacata attaaagtga cagacagaat aaatgagcaa
    16621 acatcatttg aagatgctaa agaaactata aaagaccaac tgttgaaaaa taaatatcaa
    16681 gaacaaatag aaaaattaac taaagaagct aaggtagaaa aagatgaaaa agtaataaat
    16741 aaaatcacaa tctaagactt aaaaaaagag taatatgctt aaaaaagcta tatctggata
    16801 agatatagct ttttttgtat atttttctta acaatggtaa aaataaactt aaagtaattt
    16861 atggagggat taaaaaatat gaaagcaaca ggtatagtta gaagaataga tgatcttgga
    16921 agggtagtta tcccaaaaga gataagaaag acattaagaa ttagagaagg agacccttta
    16981 gagatattca cagcaaaaga tggagaagtc atacttaaaa aatactctcc aataggtgaa
    17041 ttaaatgaat tttcgcaaga atatgctgaa acattagctg aagtactagg atatggagtg
    17101 ttgataacag acttagattc tataatagct gtatctaagc ttcctaaaaa agattacaaa
    17161 gaaaaaagta taagtgatga gttagaagca ataataagtg aaagaacagt aaaatattca
    17221 aaagataaaa acattttacc attattcaag gaagaccaaa aagaatatac tagtcaagtt
    17281 atcatgccta taattagtgc atctggtgac tgtataggtt caattgtagt tgtttcaaag
    17341 gataaagata caattgcaga aagtgaagaa aaatctttaa aaattgctgc taacttttta
    17401 ggaaaacaag ttcaataatt ttatcataaa gaccttttac atgaaggtct tttgaagttt
    17461 ttgtgtaaaa tattgaatat agcattaaaa atatggtata ttaaagtttg ttatataaac
    17521 agtatggagg ttatgttatg aacaataaca aaaataaaga ctcctttcta aaaggagccc
    17581 ttatacttgg gctagcaggt gtaatcgtaa aaataatggg tggatttttt aggattcccc
    17641 ttggaaacat gattggttca gaaggaatgg gatactatca ggctgcttat cctgtgtata
    17701 ctctattttt aacattagct actgctggat ttccaacagc tgtggcaaaa ttggtttcgg
    17761 aaaaagttgc aattggaaat tttaaaggtg caaataagat atttaaggta tcacatacag
    17821 ttttattcat taccggtata atatcatttt gtatattatt ttttggagcc gactacatag
    17881 taacaaacgt aatgaaaaat ccaggtgcgt tatactctat gaaagccatt gctccagcat
    17941 tactatttgt tcctgcaatg tctgcatata gaggatattt tcaagggaga caagatatga
    18001 ctaagattgc tgtgtctcaa gttgcagagc aattttttag agttgtatta ggtcttactt
    18061 tggcctactt gcttatgaag agtctaggtc agacttttgg tgcagcaggt gctatatcag
    18121 gggctacaat aggttcaata gcatctatgc tttatttggt gtttgcttat atgctaggga
    18181 gaaaagagcg tagagcagag atagatgcaa gccaaagatt taaagatgaa agagtgtctt
    18241 atatttttaa aaagctttta actgttgcca ttcctataac aataggagca tcagtaatgc
    18301 cacttgtaaa tatgatagat aatgttattg taataagaag acttatggaa gcaggattta
    18361 catacaaagt ggcaaattca atgtttggtc aattaacagg tatggcaatg gccacaataa
    18421 atttaccagc agtcataaca actgcaatga gtatgagctt agttccagca atttctaaag
    18481 catatgcatt aggaaataag tcaaaagcta gaaaagatac taagagtgca gttaaggtaa
    18541 ctttacttat agtattgcct tgtgcgtttg gtatggcttc tttagcaata ccaataatgg
    18601 gtcttttatt tccacatgaa ccatcttctg taggaactat tttgtttact cttactccat
    18661 gtgtattatt tttaggatta atacagacat tgactggtat aattcaggga atgggaaaac
    18721 caatagtacc tgtaattgct ttatgtgttg gtatgctatg taaaattgta ataagttata
    18781 ctttaacagg tataccagat attaatgtac tgggttcagc atttggtaca gttacagcat
    18841 actttgtggc agccatgatt aacttactat atgttaaaaa acacatgaat gtaaactttt
    18901 ctaaaaaaga atttataata aagccattta taacagttat gacaatgttt ataatggtta
    18961 agttgagtta tggtgcttta gttggattct taggaaacag catatctaca ataattgcaa
    19021 tatgtgttgg tggaattgta tatgtagtag taatacttgg aataggtgga attaaaaaag
    19081 aagagattct aactatgcca aaaggtgata aattatataa attgttaaaa aaagttaaac
    19141 ttataagata atataggaat atatatgcaa ataaaggaaa tactatacaa atagtaaatc
    19201 tccttatttg tataataata taaaaataaa gtgaaatttg ctaataggag tgatgttatg
    19261 ggtaagataa gtattgtagg tcttggacca ggagactatt ctcttataag ccaaggtgtg
    19321 ttagaatctt taataaatag ccctaaagtt ttttttagaa cagagaaaca tccaataatt
    19381 gataaactta aagaaactat acaatacact tctttagact acttttatga aaaagaggaa
    19441 aattttgata atgtttattt gaaaatatct gaatttatca tagaagaatc agcaaagggt
    19501 gacctagtgt atgctgttcc tggtcatcca agagttgcag agaagactgt aagtataata
    19561 gagagattag ccaatgaaaa gcacatagca gtagaaatat tgccttctat gagctttgta
    19621 gacgctatgt ttaactattt ggctatagac ccatctgagg gatttaaact ggtagatgct
    19681 tttgaaatag aaaattcata tatagacttg gatacaagca tgataataac tcaggtatat
    19741 gataaattta tagcatctaa tgtaaagtta aaacttatgg aacattatga ctataatcaa
    19801 gaagtatgta tagtaaatgg agcaggtgtc aaagatttag aaagtaaaaa gtttataaag
    19861 ctttgtgaat tagacagaaa tgaaaacttt tttgactatt taactagctt atatataccc
    19921 aaaagttcta aaaagatgta taatacagta catgacttag aaataatagt taatacactt
    19981 agaagtccat ctggatgtga atgggataaa aaacaaacac atcaatcatt aaaaaattct
    20041 gttattgagg aagcatatga actttgtaat gcaattgata ataatgatat agatgaaatg
    20101 gtagaagagt taggtgatgt tcttttacag gttatattcc attgtcagat aggaaatgag
    20161 gagggctact ttgatttaaa agaagtagta aatggcatat gtaaaaaatt aattcataga
    20221 catcctcata tatttagtgg tgcaaaatta gatatgtcta agtttgagaa aacttgggaa
    20281 gaattaaaga aagaagagaa aggcgaaagt actataacag atggccttag aaggatacca
    20341 aaacatttac cagctcttat gaaggctagt aaagttcaac ataaagcagc acttgtcggc
    20401 tttgattggg acaatgtaga agatgtattt aaaaaaattg aagaagaata tcaggaatta
    20461 cttgacgaac acagggtcgg gaatataaaa tacataaagg aagagctagg cgatttatta
    20521 ttttcgatag taaatctagc tagattttta gacatagact caggagaagc cttgaactgt
    20581 actacagaaa aattcataaa tagatttgac tttatagaaa aaagtgcaat gaacttgaat
    20641 aaaaaattag aagacatgac acttgaagag atggatgagt tttggaatca ggctaaaaaa
    20701 caataaaatt gtggaaggaa atttgtcaaa aatctagaac ttttatcaca aagatagaag
    20761 tctatattat aaacattttt aggaggtaaa tatcgtgaat aaagctgaat tagtatcaaa
    20821 gatggcagaa aaaagtggat taacaaagaa ggaagcagaa gctgcgttaa acgcatttat
    20881 gagttctgtt caagatgcac tagtaaataa tgaaaaagtt caattagttg gatttggaac
    20941 atttgagaca agagaaagag ctgctagaca aggaagaaat ccaagagatc cagagcaagt
    21001 tatagatata ccagcttcta aagcaccagt tttcaaagct ggaaaaggat taaaggatat
    21061 aataaatgga taattagaaa tcttgtgaaa aagtgtggat aaatttgcca cactttttct
    21121 atagaaccaa ggaaggtgat aattatgaga ttagataaat ttttaaaagt atctaggatt
    21181 ataaaaagaa gaactgttgc taaagatgca tgtgataaag ggattgtcac tataaatgga
    21241 aaagttgcta aatcttcttc agaggtgaat attggagata cattagaaat acaatttgga
    21301 gagaagaaga tgaagtttaa gataaatgaa gtaaaagagc atgtattaaa aaatgatgca
    21361 aaagaaatgt acgaaatttt agaataaaat taatagtcct ctagtatatt aatagtagta
    21421 ttattgaaat atttactggg gggatattat ggaacagaac ataactttga aagatagatc
    21481 aaagctggtt atttcgggag tcgagcacat atactctttt aatgacaaaa gagtagagct
    21541 caaaacttct gtaggcgaga tggtaattga aggcgaaaac ctagatatga gcaagctaag
    21601 tatagacgaa aatataatta gcatagatgg aactataaat tctatggtat atgcaaagcc
    21661 taaaaagcca caagagagtt tcttgaaaaa ggtatttaga tagatgatac cttttactca
    21721 agatgtaagc attttttatg ctactattta tggtggaata ctcatagggg tgttgtttga
    21781 tttttataga gggctaagag ggaattttaa attcatcaat tattttgcta taatctttga
    21841 tgtattgttt tggtttctag ctacggttat aatattcgta acaataaacc ttacagaatt
    21901 ttttgattta agatattatc attttgtggc tttgtttatt ggttttattt tatactataa
    21961 caccataagt aaaattgttt tatcaattat caataaaata attcgttttg tgagaaattc
    22021 ttttaaaaaa gttacacact acatagtcag ttttttaaat aatttgtatt acgttataat
    22081 ctattcacta cacctactat ttgatattat attttatata ccaaacatat ttatagcaac
    22141 aagaaaaagt ataaaaagaa aatcaaataa aaaattaaag aacaagaaaa aatcaaaacc
    22201 taagaagaaa aaaaataaaa ctaaaaaaag ggtgtaggaa aaatgaactt aagaaaaaaa
    22261 ttttcaggac agattattgt tatcagttta tttttaggaa tatcaatttt tagtatgatg
    22321 acaggatttg tatttgagta tacaaaagct aaggaatata aaaaagaaat agcaagtcta
    22381 aataaacaat taaaaaagac tgaaatacaa ataaacagct taaagaaaga tgaaaaatca
    22441 tatgaaggag acttagagga tatagcaaga aaaagattga atatggtaaa accaaatgaa
    22501 acagtttatg tagacataaa tcgttgatta aaacttgcta ataagtttac agatgtattg
    22561 attttaaaat ttaagattta ataaaaaagt cattcagtta aggatggctt tttgttttta
    22621 ttgaggaggg aatttcataa tgaaagtagg agctatagat attgggacaa actcaatgag
    22681 attattgata gcagattata gagatgggaa ttttataaac agaaaaaaat ttgttaatac
    22741 aactagaatt ggttatggtg tggatagtaa aggatatata aaagaagatg ctatcaaaag
    22801 aaatataaaa gcattaaaag aattttctga taagtgtaat gatgaaaagt gtgaaaaaat
    22861 ttattgtatg gggacatctg ctcttagaga cagtaaaaat ggagattact ttgttaaatt
    22921 agctaaagaa gaaactggaa tagatgttga tatagttacg ggaatagaag aatcaaattt
    22981 gggctttaag ggtgttttag aaggaataca aggtgatgat actattattg ttatagatat
    23041 tggaggaggt tcaacagaat ttattgtagg tgattcagaa ggaattatat ttaataaaag
    23101 tgagaatgta ggtgctctta ggatgacaga gaggtttttg aagcacgata ttattgaaga
    23161 tgaagagttt gaagctatga gttcatttat atatggtgag attgataaaa cgttaaatca
    23221 tataaaagac agaggattaa aaactcttat aggtatagga ggtacgatta catcattatc
    23281 agcaatgaat caagaattag agatttactc tatggaaaaa atacataata gtaaaataca
    23341 caaaaaagaa atagaagaaa ttctacaaaa attaataaag atgacattaa gtgataaaaa
    23401 gaacttaaaa gggctacaag aaaaacgtgc ggatattata actgctggtg taaaaatatt
    23461 gaatataata atggaaaaat tagaaataga agaaataacc gtaagtgaat acgataattt
    23521 ggaaggctta atatgtcaaa agtcaaaaaa aatgtattaa tattttttga tttttaaaac
    23581 cgattatgac aaaaaaaatt actcctcttt gttacaattc ttaatataaa caaaagagga
    23641 ggttttttta tgcaaagaag tgtagcagtt gtgaagaata agactgtaaa accaaatgtc
    23701 ataaaattaa taagcggata tattgatttt actacggcgg cattcgctgt gatgggattt
    23761 ttacttagta ggtctattat agtagactct gtagctcctt taggaattgc cttttttata
    23821 tgtgcatcaa agattgataa gtacaagata cctgtattca tatcaacatt attaggaata
    23881 ttgttatcat ctaatgattt tggaaatgta gcaaaatatg ctgtgtgcct tacaatattt
    23941 atggctatga ataaaaaatt aaaattatca gattcaattg ctaaggttgc atttataggt
    24001 gcaataatac tacttccaat ctccatggga caagctttct tttcaaatag gtatgtatat
    24061 ggtcttctta tagcaggagt agaagcaata ataatgttta tatctatata tgtgttttct
    24121 tatgggatag gtttaatggc caatgtcaac aataggatgt ctgtacggac agaagaagcc
    24181 atatcaataa gtttactgct aacctttagc ataatgggaa taggagatat atctctgttt
    24241 ggaatatcaa taaggaccat tttatcgacc gtattgatat tgctgacctc aatagtaggt
    24301 ggagctacca tgggagcatc tagtggtgta attattggtg tagcatccat tttaaataat
    24361 atgacatcag ctgtttatat ggggatatat tctttttcag gattaataag tggagcattt
    24421 aataaaataa ataaatattt ctgtatacta ggatatattc taagttggac aataatatac
    24481 ctatatactt ctggtattac ttctaatatg atgcaattaa gagatatact attaggttgt
    24541 ttgatagtac tagtattgcc agagcgatta tttaataaga ttgaaaaatt aataaaaagt
    24601 aatgtggctt caaatgaaat cgtatacgat tatattatga gaagcaaaaa cctaactaat
    24661 agcagattga acagtattta taaaacttat gatgaccttg cagatacatt tgataagata
    24721 agagaaaaag acaaagtgct agaccaaaga gacatagcta atgtaataga tatgattcat
    24781 aatgatgaat gtaaaagttg tagtatgaga agaatgtgct gggagtcaag atttaatcat
    24841 acatacacta tgatatataa cattcttgaa aaaattgagg agaaagggga gttaagtcta
    24901 aacgatatac ctaaaaattt tagaaaggaa tgtatgaaac cagagtcaat agtgaaaata
    24961 tcaaatcact attacaaaat gtttgtatta gattatgatt ggagtgtgaa gttttcagaa
    25021 agtagaaagt taatagcaaa tcagataaga agtatttcaa aatccattaa atccttatct
    25081 caagatttag aaggcgacat aatgctagat atagaaaagg aaaaaaatat atatgaacag
    25141 ttagaaagat atgatataac agttgataaa gtaagttact taacaaaaag taacagtgag
    25201 tttgaaatat caatagaaaa gaaaacttgc catgatggat gtatgtgtga agataaaatt
    25261 gtaaatataa tttcagacct agtaggggaa aatatgtctg ttagaaaaat aggatgtcac
    25321 tgtttaggag gtaaatgcaa ggcaacattc gttaaatctc aaaaatataa ggctgttaca
    25381 gaagtttcag caatgtcaag ggatgggcat atcctttgtg gagacaatta cacatacatg
    25441 gagataaatg atggtaaata catgatggct ataagtgatg gtatgggaaa gggtaaaaaa
    25501 gcatatgaag aatcttctgc cactattgat atactggaaa aaatgataga tgcaaaaatt
    25561 aaagatgaga tagtcattga tactataaac aatatgttgt tgttaaaatc ttcagaagag
    25621 atgttttcaa ctctggattt gggaatttta gatttaaaac gaggttgttt agaaactata
    25681 aagatgggag cgtgttctac ttatataaaa agagaggatg gagaagttga cttaatatca
    25741 tcatcatcat tacctgtggg tatattatca gatgtaaaaa ttgatagaaa aaatgtaaaa
    25801 gttaaggaag gagactatgt tataatggtt tctgatggaa ttgtagatgc aggaagaaat
    25861 aacaatttag gagacaattg gttaatatac ttcttaaaga atatagagac tacaaatcca
    25921 aaggaaattt caaatttaat attggataga gcattagagt tacaagcgtt acaaatcgaa
    25981 gatgacatga ctgttttagt tacgaaaata tgtacaaaat aaggtttaca agattatgtg
    26041 tataagatgt ggataacttg tgtataatat gttaatagac ttttgaaaaa agtatagtta
    26101 ttaacaaaaa taacatataa atcaacaaaa tttgttgata agtatggcag aaatgttaat
    26161 aaaatgtaaa aaaagaaaaa acaaaagcta ttatcaaaag agattcatgt tgaaataact
    26221 aaatttctaa gtaaagaaaa cctgtgaata tgttgattat tatgtggata actattaatt
    26281 cgatatttat ttatatagta tgaataattg aataatatat ctaaaaaaag ccatcctatt
    26341 attaagtaaa cttattgaaa ggatggcttt ttatgaatag aaaattgtgc aaagcaataa
    26401 tacctttatt tatttctatt ttttgcatgt taataataag tataaaagct gaccaaggat
    26461 atcaaaagaa taatgacaga gcaattactg tcatgaataa taatgatact gaagatgatg
    26521 ctgaaaaaaa acaagaaata gactatgaaa aaacatgttg gaatttgcaa tctttgttta
    26581 aaagtgatga ccaatggaaa aaagaactta agagttttga taaagatact aaagagttga
    26641 aaaattacat aggtaaagta acaaaatcgc caacacattt gtcttttgcc ttaaacatta
    26701 aagaaaaact tgatataaga ctaaataaac tttctgccta tgtaaaatta aaacaagata
    26761 ctaataaaaa ttcgtataag tacttagata tgaatgatag tatgagtaaa tcctatggga
    26821 attatatggg gatttgttca gatttagagt tagaaatact aaaattatca aacaaagatt
    26881 acaagaaaat tatttcaaat aaaaatataa ataggaaata tggtgcatat ttaagagata
    26941 taagaaggaa taaagtacat tatttggatg ataaatcaga agatattcta agcaaagtat
    27001 cttcaataag tagtcttcca agccaagtgt atgaattatt tagaaatatg gatagaaaaa
    27061 ctaatttgac accagctcaa tatgcgagtg aattagagtc atcagataga gaaaatagaa
    27121 aaaaagctta tcaagatgaa cttataactt acaatgataa tataaataca attgcaggtt
    27181 taattactgg tcaagttaaa aagaatattt tttattctca ggaaagaggt tatgagtctt
    27241 ctttagaaat gtacctggaa tctgataatg tagatgaaaa agtatacaat aatttgataa
    27301 ggactgtaaa tagtaatatg gacagtctac acaaatatat agatttaaga aaaaaagtat
    27361 taaaattaga taaggttcat tcatatgata tgtttgtgcc tatggttaag gctgttgata
    27421 ataatattaa ttatgaaaag gcacaaagta tagtatattc agcgttgtca cctttaggaa
    27481 aagaatacaa tgatgtagta tataaagctt tcaatgaaaa atggatagat gtttattcac
    27541 atgaaaataa agtaagtggt ggatactgct tatctgttta tgaaaatcat ccatatatac
    27601 ttttaaatta taataattca ttaggttcag tttcgacttt atcacatgag cttggacatg
    27661 ctgtatatga atacttaagt tctaagaatc agaattattt taattcaaat ccgtctatat
    27721 ttactcacga agttgcatct acaacgaatg aagcattact ttacgagatg cttataaaag
    27781 aagctaaagg caatgatgaa aaagcttatt atataacaca gtatttagac ttgataaaag
    27841 atactcttta tacacaaact atgtatgctg aatttgaaaa aactatacac gagttagtag
    27901 aaaaaggaaa aagtgtaaac gcattagtat taaatgatgt ttggggacaa cttcttaaaa
    27961 aatattatgg acaaagctat gaattagacc aattatctaa ggtcggatgg gctagaatcc
    28021 cacattttta taatagtttc tatgtctata aatatgctac aggttgtagt gcaggagtaa
    28081 gttttgccca agacatccta aagaatggtt cagataacta tatgagcttt cttaaaaagg
    28141 gaagttcaga ttatccaata aacttgttaa aatcaagtgg cataaattta actaatacaa
    28201 aacccataga aaatacaatc aaaaaatttg atagtttagt agcagaactt gaaaagctat
    28261 tataaacatt attgtaatcc acatggtaaa tatatcgaat taatgataaa atatactaaa
    28321 agtttaaagt cctatcaaga taggactttt tatttattaa ttagtgggag atgattctat
    28381 gcaagttaaa gttaaaaagg ctgtaatacc agcagcaggt ttaggaacta gatttttgcc
    28441 agctacaaag gcacaaccaa aagagatgtt accaatagtt gataagccaa ctctacaata
    28501 cataatagaa gaagctgtgg cctcaggaat agaggaaata ctaataataa caggtagaaa
    28561 taaaaaatct attgaagacc actttgataa atctgtagaa ttggaacttg atttagagaa
    28621 aaaaggtaag aaagaattgt tggaggtagt acaaaacata tctaacatga ttaacataca
    28681 ttatataaga caaaaagagc caaaagggtt gggagatgca atttattgtg cgaggcattt
    28741 tataggtgat gagccgtttg cagtaatgtt aggggatgat attgtagata atgatgtacc
    28801 ttgtctaaaa caactaacag atgcatatga ggaatataga actacaatat tgggtgttca
    28861 aaaggtaaat caagaagata ctaataaata tgggataata gaagcaaaaa atatagaagg
    28921 tagagtttac aaggtaaaag acatggtaga aaagccagag tcaggaaaag ctccttcaaa
    28981 tattgctatt ttaggtagat atataataac tcctgaaatc tttgatattc ttaaggattt
    29041 accaccaggt aaaggtggag aggtacaact tacagatgct ttaaaaatat tatctaagaa
    29101 agaagctatg tatgcataca attttgaagg caagagatat gatgttgggg acaagctagg
    29161 atttttagaa gctactgttg actttgctct aaagaaagaa gaccttaagg aagattttat
    29221 aaaatatttg aaacatgtat gtagtgaatt tgataaaaat aacaatgtgt tatacaataa
    29281 tacagatgaa aaaatagatt taattgaagt caatgaagct agagaacttg aaattcaaaa
    29341 ataattaatg aaaggtgatg tatggtggca aaatcaagtc agaaaagaat taatagaata
    29401 atagctgttg ggattgtggg aatcctcgta ataacgagta taacagctgt tttgagtatg
    29461 attgcaacta tgtaataaga taaatgacag cagttgatat acataaatat aaaaaccata
    29521 tataacttga tgtagtaaaa aacttacaat attttagtta tatatggttt ttagaattta
    29581 taaatgtaaa aaaaaatttt tataatagtt gcatatttta aaggaaaata ttattataag
    29641 atataaatat agtgataaat ttataaaaac gtgctaagat tggacttgaa atacagtaat
    29701 atagtaggta ataatgtttt aaattttatt tattgaccat aatatataaa taatgatatt
    29761 attagactta actgtttaaa taaaactttt ctttttcatg ccttcaattt ccaaaataag
    29821 tatgtatata aatgaatata tagatgagga ggattttttt gattttagat gataaagtat
    29881 tagaaggatt aggtagtaag accttagttg agctacgaga gatagctaaa gaattaaaaa
    29941 taaaatcaat tactacatat aagaaaaatg agttaattga gattataaat tccaaaagta
    30001 aaaatgagga aaaagtaaac gaagagacta aagaaaaaaa tgaagatatt aaagaagaca
    30061 taaagaatag aaaagaaaat atagagcgtg aaagtataga aaaagaagtt gaatataaga
    30121 gccctaccaa atcatacaac aagaaagaaa tagataccca aaatatttcc caaaaccaag
    30181 aacatagaaa tcaaagaaat gactataata agacaacgag tgtaaatgat agcaataagt
    30241 catcaaatat atctaataga atggtcagaa ataataataa aaactattat atgcctaaac
    30301 aagttgatga atctaagata gtagatgaat ttaacacatc aaaagaggat gaagtagtag
    30361 gtgtacttga aattctgcca gatggatttg gttttttaag aggttcaaat tatttgtcta
    30421 ctgaaggaga tgtttacgtt tcaccatctc aaataagaag atttaatatg aaaacaggag
    30481 acaagattaa gggaataact agacatccaa aaagtggaga gaaatttaga gcacttttat
    30541 atgttcaaaa aataaatgat gaaaaccctg acacagctat acaaagaaat gcgtttgaga
    30601 cattaacacc aatatttcca gaagaacgac ttacactgga aactaataga aatgaaatag
    30661 ctacaagaat tatagattta atttcaccta taggaaaagg acaaagagga ttgatagtag
    30721 cacctccaaa agctgggaaa acagttcttt taaaaagcgt tgcaaatagc atagctaaaa
    30781 atcatccaaa tgttgaactt atagtactcc ttatagatga aagaccagag gaagttactg
    30841 atatgaaaga atctattgaa ggagacgtta tatattccac ttttgaccaa gtatcaagtc
    30901 atcatgtcaa ggttgcagag atggtgttaa atagagctca aagattagtt gaacatggta
    30961 aagatgtagt aattttacta gatagtataa ctagattggc aagggcatat aaccttacaa
    31021 tatctcctac aggtagaact ttatctggag gtattgaccc tggtgcactt catggaccta
    31081 aaaagttctt tggtgcagca aggaatatta gacagggtgg ttctttgaca atattaggaa
    31141 cagcattggt tgaaactggt tctaggatgg atgatgtaat atttgaggaa tttaaaggta
    31201 caggaaatat ggagctacat ttagatagaa aattagcaga aaaaagaata ttccctgcaa
    31261 tagatattta caagtctggt acaagaagag atgatttatt gcttgatgat gaagaaaaga
    31321 ctgctctttg gagattaaga agagaaatga gtaataattc agtaatggaa attacagata
    31381 aagtcattga acttataaag agaactaagg acaataaaga gtttgtaaaa tcaataaaga
    31441 atttgtaaaa tcaataatac aaatgcaaat atggattata taaactaata gaaaaatcac
    31501 ttgaatggca taataagttg tgctataata atgtagttga taatgtaaaa agaaagttaa
    31561 tataattaat ttttttaaat aaagagaaga ggtgactata atgcaaaagg aaatacaacc
    31621 aaaatacaat ccagttgaag tgcgttgtgc ttgtgggaac acatttgttg ctggttcaac
    31681 taaggacgaa ataaaagttg aaatatgttc agagtgccat ccattctata caggaaaaca
    31741 aaagaacata gaaaaaggtg gaagaatcga caaattcaag aaaagattca aaatggacta
    31801 atttttataa aagtggagtt ggaggaagcc aactcctatt ttattacaaa aaaacattca
    31861 tattctactt taaattgtct ttttgtatta taataataaa tatgtaaaat tgagtattct
    31921 aaatacaata aaatgaggtg agtggatggc aaaacaagct gtaggtgggc aagctgttat
    31981 tgaaggtgta atgatgcagt caaaagacaa aagagcggta gctgttagaa aaagtgacgg
    32041 agaaatagca ctaaaagaag atagaataaa aagttgggta agagataaga atatagataa
    32101 gatacctttt gttagaggtt cttttgttat gatagataca atgattcaag gcataaaaag
    32161 cttaaacttt tcttcagagt tttttatgga agaagctgaa gaggataaat ttgatttatt
    32221 tataaagaaa atttttaaag ataaggcaaa tgatataatt ataatatttt cattggtcat
    32281 agcaatgtta ctttctgctg gattattcat ttttatacct acattggtag gaggagcttt
    32341 ttctaaagta atgcctaatg attttatgct taatttaata gaaggaatca taaggatagc
    32401 tatattattt gcatatatag tattgatttc acgaagtaag gatatagaaa gagtctttca
    32461 atatcatgga gcagaacata aatctatata ttgctatgaa aatgatttag aacttacagt
    32521 agagaatgct agaaaattta aaagattaca tcctagatgt ggtacaaatt tcctatttat
    32581 agttatggct gtatcaataa tattgtttgc attttttggt tggccaaatc caattcttag
    32641 aatatttatg agaattatat gtgtgcctat agtagcaggt ttatcttatg aggtaattag
    32701 agttcttggg aaatatgata atggatttac taaaattatt gcatatccag gaatgatgtt
    32761 gcaatatttt acaacaaaag agcctgatga tgagcaatta gaggttgctt tggaagcatt
    32821 gaaagcagta gtagattaga gaaaaggata tttgttatga cgataaaaga tataattatt
    32881 aaatattcag ataaactaaa agatataagt gatactccaa gattagatac agagttattg
    32941 ttacaaaaaa cattgggtgt tgatagatta tatattcact taaatctgaa taaagagcta
    33001 actgaagaac aaaaaacaaa atttatgggt tttgcagaag aaagattaaa tggaagacca
    33061 atagcatata tagttgaaaa tagggagttt atgggattag atttttttgt taaggaaggc
    33121 gtattgattc caagaccaga tacagaaact ttagtagaag aaataattga aatatgtaga
    33181 gaaaaaaaag atgtaagtat attagatata ggaactggtt ctggagctat tacaataagt
    33241 ttagcaaagt acattgaaaa ttctaaaatc atgtcttttg atatatcaga gacagcatta
    33301 gaaatagcta aaaaaaatgc tattataaat gaagttggtg aaaaaataaa gtatattaat
    33361 tctgatttat ttacagcaat aagtgatagt aacataaaat tcgatatcat agtttcaaat
    33421 ccaccatata ttaaaaaaca agatatagaa actctgcaca aacaagttaa agattatgaa
    33481 ccatataatg ctcttgaagg tggagaagat ggattagact tttatagaag aataacagaa
    33541 caaggtaaga aatatttaaa taagtgtgga atattagcat atgaagttgg tcataatcaa
    33601 gctgaagatg taataaatat tatgaaaagt aatggatata aaaaaatata cacaaagaag
    33661 gatattcaag gcattgatag agttgttata ggttacaata tatgatataa tcaacaattg
    33721 atatgttaca tttaatcgag gtgaaagtat gttaaaaaaa ttagaggtat tggaagatac
    33781 atataaagac ttaagtgaaa aaataggtga tccggatgta ataaatgatc aaaaagtttg
    33841 gcagaaatat ataaaagaac atgctgactt ggaacctatt gttatgaaat acagagagta
    33901 taaaagcgtt ttagatagta taaaagaatc aaaagaaatt ttacaagaag agtcagatga
    33961 agaattaaga gaattagcta aaatggagtt agctgagatg gaagaaaaag tagcacctct
    34021 ggaggaagaa ataaaaatac tattattgcc taaagaccct aatgatgata agaacgttat
    34081 agttgaaatt agaggtggag ctggaggaga tgaagcagct ctatttgctg gtgatttatt
    34141 tagaatgtac tcaaggtatg ctgaaagaag aagatggaaa atagaattat tgagtgcaag
    34201 tgatactgga gttggtggat ataaagaagt atcttttatg ataaaaggta aaggtgctta
    34261 ttcaagatta aagtatgaat ctggagttca tagagttcaa agaataccat caactgaatc
    34321 tggtggaaga atacacacat caacttctac tgttgcagta ttaccagaag ttgaagatgt
    34381 agaggttgaa ataaatccaa atgatttaag aatagatgtt ttccgttctt cgggtaatgg
    34441 aggacaaagt gtcaatacta ctgactctgc tgttagggta acacatatac ctacaggtga
    34501 agtagtatct tgtcaagatg gaaaatcaca attaaaaaat aaagagcaag ctttaaaaat
    34561 attaaaagct agactttacg ataaagcatt ggcagaacaa cacaaagata tagcagcaga
    34621 aagaaaaagc caagtcggaa caggagacag gtcagaaaga ataagaacat ataatttccc
    34681 tcaaggtaga ataagtgacc atagaataaa tttaacttta tataagttag atgcattctt
    34741 agatggagat atagatgaaa tgatagatgc tttaattact gttgaccaaa cagaaaaaat
    34801 gactgcaata taagtaatgt tttaaagaag ctgtttcttc ctagaaatta ttttctattt
    34861 tgaaacagct attttgcatt ttaggtcatg ttatcatatt ctattgtata taattgttat
    34921 atacaagtat aggagggtta tatgatacta aaagtaacaa taattgggct tttggcaggt
    34981 gttataggga caggattagg aggagtaata tcagccattt ttaaaagaga ggtcgataaa
    35041 tatctaagtt tttttatggg attatctggt ggaataatgt tggctgtagt ggtgtttgat
    35101 ttaatgaaag aatccatgga caaaatgggg ataatcaata cagttatatt tacgtttgtt
    35161 ggagctctca taactatgta cataaaaaca aaattagatg tgtctggaaa tatggcatct
    35221 ggatatctta tattcataag tatattacta cataatttac cagaaggact agccattggt
    35281 tcttctttta tgtcaactga aagtttaggt attacacttg ctatagttat aggtctacat
    35341 aatataccag aagggttagc tatggcatta ggcttagttt gcaataaaat gaaactaagt
    35401 aaagttattt tgtttactgt aatagctgga ttacctatgg gtctaggaag ttttttaggt
    35461 gtttattttg gaggagtatt tacttctctt ataggagtat ttcttgctac agcaggaggt
    35521 actatgatgt atgttgtatt ggaagaaatt tttccacatt caaaaagtgt gtattgtata
    35581 attggtttct tgttaggtac aatgattgta aattatatat aaaattaaaa tgaaagcagg
    35641 catacaaaag atgaaaacta ttataagtaa tattgatata aataatattg attatgagga
    35701 aataaaaata caagcaaaac ttcttagaga aggtaaaaca gttatttttc caactgagac
    35761 agtatatggg ttgggtgcaa atgcattaga tgaaaatgca gtaaagaaga tttatgaagc
    35821 taaaggaaga cctagtgaca atccattgat agtccatata tatgaaaaag aagaagtata
    35881 tgacttagcc aaagatataa gtgataaagc taaacttgtt atagaaaagc tttggccagg
    35941 acctataaca attatattaa ataaaaaaga tataattcca tacaaaacta gtggagggct
    36001 tgatacagtt gcaataagga tgccttctaa tgttattgcc agggcaataa taaaagaagc
    36061 tggtatacct attgcagctc cttctgcaaa tatctcaggt cgcccatctc caacaaaagc
    36121 aaaacatgta tatgaagaga tgaatggaag agtggatgga atagttttag gaggagacag
    36181 taattttgga ttggagtcaa ctgtattgga tttaacagaa gagactccta tgatactgag
    36241 accaggaagt ataactaaag aagttttaga ggaacttctt ggagaagtaa agcttgaccc
    36301 atcattaagt aaaaaagaag ataatcaaaa agctaaagct cctggaatga aatataaaca
    36361 ttattctcca aatgcagatg tatatataat atctggaaaa agagaaaatg tagcatcaaa
    36421 gattaatgat atgataaaat ctaatagaga caaaggtcta aagacaggtg taatgtgtat
    36481 tagtagaaac cgagagtttt atgatggaga agtaatcgat ttaggaaact ctcttgaaga
    36541 agttgcaagt aatctgtttg atgctcttat agaaatggac aaaaagggtg ttgatgtcat
    36601 atattcagaa gagttcccca agactggagt tggacaagca attatgaata gactactgaa
    36661 gtcagcagga tacaaagtga taaaggctta agagggagtt gtaaaatatg aatatactta
    36721 ttgtgtgtac aggtaatact tgtagaagtc ctatggctga ggctatacta aggaaagcaa
    36781 ttaaagaatc tggaagaagt attgaagaat attctatatc atcagcaggt atatctacag
    36841 caaatggaat gggtgcttct gaaaattcta tagaagtact aaaagaaatc ggtatagact
    36901 tatcaaatca tagaagtaaa gtaattacta aaaaattaat tgatgaatca gatataatac
    36961 taacaatgac taaatctcat aaagaaattt tagtacaagc agttccaaaa tgcaaagaaa
    37021 aagtgtatac ttttaaaggg tttgcaaata aaaatgagga agatatatct gacccatttg
    37081 gtggaaattt ggatatttat agaagcacta tgagagaaat aatgtattct gtcaacgaaa
    37141 tagtaaaaaa aatatgaaac ggaggataaa caaatgaaga taggattagg ctgtgaccat
    37201 ggaggatata atttaaaaaa agaaataata agctatttag aaagtaaagg tatagagtgt
    37261 gtagattatg gaactaataa tgctacagat tctgttgatt atcctgttta tggagaaata
    37321 gttgcaaatt cagttataaa taaagaagta gattatggaa tattatgttg tggtacaggg
    37381 ataggaatat ctttagctgc taataaggtt cctgggataa gatgtgcagt tgtatcagat
    37441 gtattttctg caaaaatgtc aaaagcacat aatgatgcaa atatgttatc attaggcgaa
    37501 agagttttag gaaaaggtct agcacttgag atagttgaag catggattaa tacggatttt
    37561 gaaggtgata gacatgctag aagagtaaac atgattaaat caatagaaga aaaacataat
    37621 aaataataaa aattacagtt ttatcaggag gaattatcaa aatgagcaaa gtggtagaga
    37681 caaaccatcc attaatacaa cataaattaa ctttaatgag agataaaaat actggttcta
    37741 aagactttag agagctttta actgaaattg caatgttaat ggggtatgaa ataacaaaag
    37801 atataccatt aaaagatgta gaaatagaaa ctcctattca aaaaacatca tctaaagtag
    37861 ttgcaggtaa aaaattagca ataattccaa ttttaagagc aggtcttgga atggtagatg
    37921 gattagtgag cttaatgcca gcagcaaaag ttggacatgt aggactatat agagacccag
    37981 aaacattaaa accagttgaa tattattgca agcttcctca agacatagga gaaagagata
    38041 taatagtagt tgaccctatg cttgcaacag ggggttctgc tgtagcagct atagatttac
    38101 ttaagtctaa aggagctaaa agtataaaat tagctaattt agtagctgca ccagaaggta
    38161 tagcagaagt gcaaaaatat catgatgatg ttgatatata tgttgcatca gttgacgaaa
    38221 gattaaatga acatggatat ataattcctg gtcttggtga tgctggagat agattgtttg
    38281 gaactaaata aaaataaata actataaata tttattaggg actttcttat atagtgtata
    38341 taattttatg taagaaggtt cttttttata tattttttct caaaatagga attattgaat
    38401 tgataaattt ctcgaattga gaataaaaag tttttaccaa ttaaaaaaga ttgcttattt
    38461 tacaagattt tatagaatag tagtctattt taatgtggca tgttagttgc ttacgattaa
    38521 gattataggt attaagtgta ttagttaaat tgagaattga atattaataa tattattggg
    38581 agtggtaata catgaagaat tgtatagtag taagaggggc tggagattta gcaacagcag
    38641 ttattcataa attacacaac agtggtttta aagtagttgc gttagaaata gaagagccat
    38701 tagctataag aaggcaaatt tctttttgtg aggctgttta tgaaaaaaaa gtaactgttg
    38761 aaggtgtaga atgtatattt tgcaatactg aaaaagaaat agaagaagtt ttatctcaac
    38821 ataaagtagc cttgattata gacccaaaag gagaaaaatt atctaaatta aatccaatcg
    38881 ttttggttga tggaatactc gctaagaaaa acttaggtac aactaaagat atggcaccac
    38941 ttacaatagc tttaggtcct ggattttgtg caggagaaga tgtagatgtt gttatagaga
    39001 caatgagagg tcataatcta ggaagaataa taactaatgg tagagcagaa aaaaatacag
    39061 gagtaccagg agcaataaaa ggtttttcta aagagagagt tatgcattca cagtatgatg
    39121 gcataatgga aaatgtatgt aagataggtg atatagttga aaagggagac ctactttgct
    39181 atattaataa ttatgaaaaa aaacatgaag ttagagcaac aatatctgga gtccttagag
    39241 gtttattaaa ggatggagca aaggtaaaca aaaatttaaa aatactagac atagacccta
    39301 gaaaagaaga agttaagaat gcttttacaa tatcagataa agctagatgt atagctggag
    39361 gggttttaga agccatacta aacaatggga tttatcccca aaattaattt atattagata
    39421 aagctattaa tattttatta agatgatata ataaatttaa tagattaaag agagcatatg
    39481 acgggtattt aaaatttaaa tggtaaaaat gctctttttt tattggacat tcaattaaca
    39541 tgtgtatata atataaaaat aagtatttat ttaggttgaa atctaataaa tatataatac
    39601 cagtacataa gcaaagagga gaaaagtaaa ttatgagacc atcatgggat gaatacttca
    39661 tggaaattgc agaagtagtg aaaaagcgtt caacttgtat tagaagacaa gtaggagctg
    39721 taattgtaag agacaaacaa atattaacaa ctggatataa tggttctccg agaaatcttg
    39781 aacactgtga aaatattgga tgtaagaggc aggaattaaa tattccctca ggagaaaggc
    39841 atgaattatg tagagcatta catgctgaac aaaatgcgat aatacaagct gctcataatg
    39901 gtattagtgt agatggggca actttgtatg ttactacaag accttgtgtt ttgtgtgcaa
    39961 aaatgtgcat aaatgcaggt atattgaaaa ttgtttatga aggtgattat ccagatgata
    40021 tgtctacaga attattaaaa gaagcaggaa tagaactcat aaaattttag tggataaaat
    40081 ttataagaca ataaatgtca aattatgaca ctgaaataca atggtatcaa atgattaaaa
    40141 gaaagtattt gaaaaatcat cagattagta gtaagatata ttttataatt aaataaattt
    40201 atgagataga ttaattacat gttatttatt ttataataat gagggatact atttatgagt
    40261 aaaagaaata cacatacaga aattgcaaag ttgtttagtt taataagtca aataggattg
    40321 atgattgtaa tatcaatatt aggatgtact ttcttaggaa aatttttaga tagtaaattg
    40381 aatactactc cagttctgac aataatattt ttattgttgg gtgttggagg tgcttttagt
    40441 ggtgtttata aaactctaat agtatataca cgaaggaagt gatatattgg atataaaatt
    40501 acttaaccag gtaaggtcag taacaaaagg tatagtaata tttgatgtag ttttaattgt
    40561 gctaatgttg ataacatcaa cattaagtaa accagtatta ttaggactta tatttggctc
    40621 tataatagct atgttaaatt ttagattatt ggcgatatca ctggaaaaat cagtgacatt
    40681 tcctgctggg aaggcacaag cttatgcatc agcacaatat gctgttagat tatttataat
    40741 ggcagttgtt ctaattgcat cagctacagt accacattta aatataatcg gaacagcatt
    40801 gggattattg agcacaaagt ttgtcatatt atcaaaaaac tttataaata aattaaagag
    40861 gaaggaggca taagtatgaa tcaagcgcat tattatctca taggaaactt taagattagt
    40921 gaaacaatac tagtaagttg gggaataatg gcctttttag cgatagcatc ttattttttg
    40981 actagaaact taaaaaaagt tccaactagt aaagttcaaa tattcttaga atttgcagta
    41041 ggtggtcttg ctaaaatggt tacagatgtt atgggtaaaa atacagtaaa aagaatgcca
    41101 tatatgatac catacatagg aagcttgttt ttatttttcg catgttcaaa tttaagccca
    41161 ttacttggat taaggtcacc aacaaccgac ttagacacaa ctgctgcatg ggcaatgatt
    41221 acatttttta tgatttattt tgcaggagtt aaatttaagg ggttatcata ctttaaagaa
    41281 ttggctcaac cacttgccat aatgttacca atcaatatta taggtgagtt agctagacca
    41341 atatcattaa gtttccgtcc atttgggaat attttaggtg gaacaataat aatgggtctg
    41401 ttttatcaac ttttaggttt tatatctagt ttaataccag gattatcaat accaattgga
    41461 gaaatattaa taccagtccc attacacttg tactttgact tatttgcagg attgttacaa
    41521 gcatatatat ttataatgtt aacaatggta tttatatcaa gtgcaggaga gttagattaa
    41581 gaaatcaact tatattaaga taaaaataaa ataatttaaa aaattaaagt tttaaggagg
    41641 aaataattat ggaaacagca atagtagcgg cagcatcagc aataggagca gggatagcag
    41701 ttgcaacagg tataggtgca ggtataggtc aaggtatagc agcagctaaa gctgcagaag
    41761 cagtaggaaa ccagccagaa gctaaaggtg atataacatc aactttatta ctaggagttg
    41821 ctatagcaga gtcttcagca atatatggtt tggttatatc aataatactt ctattcgtaa
    41881 atccattctt taaatactta ggaatgtaga tttagtcctt aaatattttg agataggttt
    41941 ataggtaaat catctcaaac ttataatcat gagatgaact ttatcatctc gtgattataa
    42001 gtagttatga tttatggaaa ggaggataat tatggaaaaa gcactagtag gtataacgtg
    42061 ggaatttgtg ttccaaattg ttaatacttt tataatattt ttacttctga gaaagctttt
    42121 attcaagcca gtattaaata taattgaatc aagagaaaat gatattaaaa gtgatttggc
    42181 agaaggcgaa aaagcaaaaa atgaaggttt agctctgaaa aaagaatatg agtcaaaaat
    42241 caattttgcc aaagatgaag gtcaagaaat cataaaacaa gcaactataa gagcagagca
    42301 gaaatcagat gatatagtaa atactgcaaa gaaagatgct cttgatataa aagaaaaagc
    42361 aaacaaagat atagaacaag aaagacaaaa ggttataaat gaaatcaaaa atgatatatc
    42421 taacatagca ctacttgcag catctaaagt tattgaaaaa gatttagata aatctaaaca
    42481 tgaagaattg atagaaaact ttataaaaga ggtaggggaa gctaaatgat aaatgtaata
    42541 gcaaatagat atgccgaagc cctatttcaa ttaggagaag aagaaaattc aactgatgtc
    42601 ttattcaaag aactagaaaa agtagtagat atgatgacta aagttagtaa agatttctac
    42661 aaagttttaa aatcaccatt agtttcaaaa tcagaaaaga aaaacttggt agaaattatt
    42721 tttagtaaag aagtaagttc taacataaaa aactttttaa aagttttagt agataaagat
    42781 agaatatcat atttagaaga tatcgaatta gcttataaag aattattaaa taagaaaaac
    42841 aatgtaattg atggagtagc aataagtgct attccaatga gtgaaacaga tattaaagaa
    42901 cttgaagtca aactttcaaa taaatacaac aaaaatgtta ctatagaaaa tgttgttgac
    42961 aagactatac tagggggagt tctagttaga attggaaatg aacaaataga tggcactgtg
    43021 aaaactcgtc ttgataagat gaaagaaaag ctatctgaag taatatctta agaatggcgg
    43081 tgaacccatg aacttaaaac ctgaagaaat aagttctata attaaacagc aaataaaaaa
    43141 ttatgagaat aaagttgagt tgacagatac aggtagtgtt ttaacagttg gggatggtat
    43201 agcgagtgta tatggattag aaaaagctat gtctggtgag ttgctagagt tcccaggtga
    43261 aatatatggt atggcactta acttggaaga agaagtagtt ggagctgtta tccttggaga
    43321 tgacagtgaa ataaaagaag gcgatatagt taagagaact ggaagaatag ttgaagttcc
    43381 agttggagaa gctttaatag gtagagttgt aaactcacta ggtcagccaa tagatggaaa
    43441 aggacctata gcgtacacaa aaactagacc agttgaatct gaggcacctg gtataataga
    43501 tagaaggtct gtttatgaac cattacaaac aggaatcaaa tcaatagatt caatgatacc
    43561 aattggtaga ggtcaaagag agttaataat aggtgacaga caaactggta aaacatctat
    43621 agttattgac actatactaa atcaaaaagg aaaagatgtt atctgtatat atgtagcaat
    43681 tggacaaaaa cgttctacta tagctcaatt agttagctct ttagaaaaag gtggagcttt
    43741 ggattataca atagtagttt cagcaacagc atctgaatca gcaccacttc aatatatagc
    43801 accatatgct ggagcagcta tgggtgaaga atttatgtac aatgggaaac atgtacttat
    43861 agtgtatgat gatttaagta aacaagctgt tgcatataga gaaatgtcat tgttacttag
    43921 aagaccacca ggacgtgagg catatcctgg agatgtattc tatttacatt caagattact
    43981 tgaaagagca gctaagttat ctgatgaatt gggtggaggt tcaatgactg ccttgcctat
    44041 aatagaaact caagctggtg acgtttctgc atatatacca acaaatgtta tatctataac
    44101 agatggtcaa atatacttac aaccagagtt attctattca ggtgtaagac cagcagttga
    44161 ccctggtata tcagtatcaa gggttggtgg ttctgcgcaa attaaagcaa tgaaaaaagt
    44221 tgcaggaaca ttaaaacttg catattcaca atatagagaa cttgcagcat tctcacaatt
    44281 tggttctgac ttagatgaag atactaaaaa gagacttgct caaggtgaaa gaatcgttga
    44341 gatattaaaa caaggtgagc atcaaccaat aaaagttgaa aatcaagtta tgataatata
    44401 tgctgttata aataatcatt tagaggatat tccgatagat aatatagcta gatttgaatc
    44461 agaattgtat gcatttgtag ataataatta tccggaaata tcaagaaaaa tattaggtgg
    44521 agaagacttc actcatgatt taacagatgc aataaatgaa tttaaagaaa agtttgttgt
    44581 tgaagtataa tcctttttaa aaaaggaggt aaattaattg gcaggagctg gaatcaaagc
    44641 aatcaaaaca cgtatgggaa ctgttgaaag tacaaagcaa ataactaaag caatggaatt
    44701 agttgcatct tctaagctta gaaaagcaaa agaaaaggct gaagattcaa gagaatattt
    44761 taatacttta tacaatacag taaaagatat tgcccaaaca acacgtggaa ttagaaatga
    44821 atttttaaaa gaaagagaag taaaaaataa atgctatgta gtagtagctg gagacagagg
    44881 acttgcaggt ggatacaatt ctaatgtatt aaaggaatct gtctctcata tgaaaggtaa
    44941 aaaggaaaca gtgattactg ttggaaagaa agcacatgaa tttttttcta aaagaaacta
    45001 caatatagca aaagatataa atagtgttga agcttgtgga tatgaagatg cgacagaaat
    45061 ttcacaaact attattgacc tgtataaaaa aggtgaagta gatgaagttt atattgctta
    45121 tacaaaattt aaatctgctt tatcacaaga agttgaaatt ttgaaattgc ttccattagt
    45181 ctttgaaaaa gatacagacg aacaaggaat ggcagtaaaa gaagaaaaac aaaatagatc
    45241 aaaagtacaa tatataccat cagcagaagc agttttaagt tatattgtac ctcagtttat
    45301 atcaggtaca gtatatggtg gaattttgga atcttttgca tcagagcaag gtgctagaag
    45361 aactgcaatg gaatcagcca cagacaatgc gaatgaaatg ctatctaatt tagagttaag
    45421 ttataacaga gcaagacagt cagctgtaac tcaagaaata acagagatag ttggtggagt
    45481 tgaagctcta aaatagggag gttaggaaat ggcaaatgta ggtaaagtag tacagattgt
    45541 aggagctgta ctagatgtta agtttgatag tgaacaaagc ctacctaact tattaaatgc
    45601 gttagtaata aagttaggag ataaagaaat agttgcagaa gttgctcaac atataggtga
    45661 tgatactgta agatgtatag ctatgagtgc aacagacgga cttgtaagag gtatggaagt
    45721 tgtagataca ggagggccaa taagtgttcc tgttggagat gaaactttag gtagaatatt
    45781 caatgttctt ggaaaaccag ttgatgggaa accagctcca aaatctgcac ctaaattacc
    45841 tatacataga ccagcaccgg cgtatgatga actagaaact acagcagaga tactagaaac
    45901 tggtattaag gtagttgact tactagctcc atatctaaaa ggtggaaaaa taggtctatt
    45961 tggaggagca ggagtaggaa agacagttct aatacaagag cttataaaca atattgctaa
    46021 gcaacatggt ggtatttctg tattttcagg agtaggagaa agaacaagag aaggtaacga
    46081 cctttatggc gaaatgagtg agtctggagt tataaataaa acagctctag tatttggtca
    46141 aatgaatgaa ccacctggag cgagaatgag agttgcttta actggactta caatggcaga
    46201 acattttaga gatgagcaag gacaagacgt tttacttttc gttgataata tattccgttt
    46261 cacacaagct ggttcagaag tttcagcact tctaggacgt atgccatcag ctgttggtta
    46321 tcagccaaca ttagctactg aaatgggtgc acttcaagag agaataacat caactaagaa
    46381 aggttcaata acatctgttc aagcagtata tgtaccagca gatgacttaa ctgacccagc
    46441 accagctaca acattctctc acttagatgc aaaaacagtt ttatcaagac aaatatcatc
    46501 actgggtata tatccagcag ttgaccctct tgaatctaca tcaaggattc ttgaccctag
    46561 tatagtagga aaagaacatt atgaagtagc cagaggtgta caatcaatac ttcaaagata
    46621 taaagaactt caagatataa ttgctatact tggtatggat gaattatctg atgaagataa
    46681 gttaatagtt gcacgtgcta gaaagataca aagattctta tctcaatcat tcacagttgc
    46741 tgagcaattt acaggaaatc caggtcaata tgttcctgtt aaagagactg taagaggatt
    46801 taaagaaatt cttgaaggga aacatgatga cttaccagag tcagcattct tatttgtagg
    46861 aacaatagaa gacgctgtta gaaaagcgaa gggaagtatg tagtcatggc cagtgaattt
    46921 tcattgaaag ttgtaactcc aagtaatact ttttatgatg gagatgttga aatgatagta
    46981 gttagaacta caaaaggaga caaaggggtg ttgaaaggtc atataccatt tgtttcaggt
    47041 atacctgtag gaactttaaa gataaaaaaa gagggaagtt ttaaagaggc gaaaatagca
    47101 gaaggattca ttaatgtaac tcaagataaa actgtcatat tgacagaatc tgctgagtgg
    47161 atttaataaa ttaatttaga gtataaaaag tcattttgac caaaaaaact tttgggtaaa
    47221 atggcttttt tgcgttttgg tgttaataaa ataacgataa aattgtaagt ttttagtgat
    47281 aataaattca caaaatgttt aaatttactg ccaaattatg ttgtaattaa taagataaag
    47341 aagatttaga tagcttttat gtaagtgaaa aagtcttcat ataagaaata catataaaat
    47401 ttttataaaa tttaaaaaat ttgcttagtt gtgtagtata ataaataata taatagattt
    47461 gtatataata ttcattacaa ataattttaa aagttacagt tttattataa tgattattaa
    47521 attaagataa gtaacataaa ctaaataata atgtgtatta tcgatagtaa atttataaat
    47581 tcattattaa ttattgaaag aagtattata aattttctgg aggaacaatg aatatatttg
    47641 atattggcgt agatataatt gaaattgata gaataagaaa ggctgtagac aaaaataata
    47701 ggtttttgga gaagatattt acagatagag aaatagagta ttttaattct aagaatttta
    47761 aagcagaaag tatagcaggt aatttcgcag ccaaggaagc tataagtaaa tctataggta
    47821 caggaattag agtgtttaat tttaaagata tagaagttct cagaaatgaa atgggaaaac
    47881 caatagtaaa aacttataat aatctagcaa aaatgtgcat agattataat gttttagaaa
    47941 taaaagtttc aatatcacat tcaaaagatt atgctatagc aaatgcgata accataatta
    48001 aggactaagg agtgattaca ttatgatgga taaaacagca aaagaaataa tgacaacaga
    48061 tgtaatagta gcaaaacaag atgatagtat agcagatgta gctaatatgc taattgcaga
    48121 aaaaatagga ggtctgccag ttgtagattc agagaataga gttgttggaa taatttctga
    48181 aacagatatt cttaaaaaag aaaaatatat agaagcacct ctctatataa atctattaca
    48241 aggattgatt ttcttagatg acttaaaaaa agttgaaaaa gatataaaac aagtagctgc
    48301 ttataaggtt ggagaactta tgtctaaaga tataataaag gttcatgaag atgataagtt
    48361 tgatgatgta gctaatgtaa tgattaaaaa atcaataaac agagttcctg tagtagatga
    48421 tgacaacaaa cttaaaggta taatttgtag atatgatata ataaaagctt tatacaatga
    48481 ataaactgaa gtaggaggtg tgatgtgaga aaaaagtgga ccatagtatg tattatgttc
    48541 ttggcactgg ttataattgt aataggatgc caaaaacgac agtccacaaa agaagaagta
    48601 tacaaagatt ttcaaaagca gatttctgat atgaattatt acagttgtaa agctgaagta
    48661 gaagtagttg gaaataaaag cccacacaat tatgttttaa ttcatactta taaaaaaacc
    48721 gataactata aattagaagt aatttcccca aaacatttaa aagggaaaag tatagaatat
    48781 caaggtgata aaatattagt taaaaatcca aagattagtg atgtagtgga gttacctaat
    48841 acaggaaaaa ataatcaata tctttttgta ggagacttta taaaaaatta tcttcaaaat
    48901 gaggagatga aagtaaagtt atccaaagga catttggttt tagagacttt tattcctggg
    48961 gataataaat attttaacaa gcaagtatta tatgtaaatg ctgacacaaa aaatcctgaa
    49021 aaaatggaag tgttagataa agagggagtg ccaagattta cagtaaaata caaagatttt
    49081 gaatacagaa actaaggggg actaaagaca tgcaaaaaat aacagtgcct acatgggcag
    49141 agataaatct agataactta agatttaact taaataatat taaaaattta ttagaagaag
    49201 atattaagat ttgtggagta ataaaagctg atgcatatgg acatggtgca gtagaagttg
    49261 caaaattgct agaaaaagaa aaagtagatt acttagcagt agcaagaact gctgaaggaa
    49321 ttgaacttag acaaaatggc ataacacttc ctattttgaa cttgggatat actccagacg
    49381 aagcttttga agattctata aaaaataaaa taactatgac agtttattct ttagaaacag
    49441 cacaaaagat aaatgaaatt gcaaaatctt taggagaaaa agcctgtgtt catgttaaaa
    49501 tagactcagg gatgactaga ataggtttcc aacctaatga ggagtcagta caggaaataa
    49561 tagaattaaa taaattagaa tatatagatt tagaaggtat gtttactcat tttgctacag
    49621 ctgatgaagt aagtaaagag tacacttata aacaagctaa taattataaa tttatgtctg
    49681 ataaattaga tgaggctggt gtaaaaatag ctataaaaca tgtatcaaac agtgcagcta
    49741 ttatggattg ccctgattta agattaaata tggtaagagc aggaataata ttatatggtc
    49801 attatccatc tgatgatgta tttaaagata gattagaatt aagaccagcc atgaaattaa
    49861 aatcaaaaat cggacatata aaacaagttg aaccaggtgt aggaataagt tatggactaa
    49921 aatacacaac tacaggtaaa gaaacaatag ctacagttcc aataggatac gcagatggat
    49981 ttactagaat ccaaaaaaat ccaaaggttc ttattaaggg agaagtgttt gatgtagttg
    50041 gtagaatatg tatggatcaa ataatggtta gaattgacaa agatatagac ataaaagttg
    50101 gagatgaggt tatactattt ggagaaggcg aagttacagc tgagcgtata gctaaagact
    50161 taggaactat aaactatgaa gtgttatgta tgatatcaag aagagttgac cgtgtttata
    50221 tggaaaataa tgagcttgta caaataaaca gttatttgct aaaataaaac catgatatta
    50281 taatctggga ggcgattttg tgtacgataa gagtaaagaa aaaatagaag ttactttacc
    50341 tgactctctg atttctgaag ttgatagtat agttcagatg gagaacagta acagagaaga
    50401 ttttgcaaaa gctgcttttc agttctatat aactcagaaa aagaaaattc atattaaaga
    50461 atctatgaaa acgggttata gagaaatggg tcaaattaat ctgtcactag ctgaattagg
    50521 tatgacagtt gaagaagtat cttctgatga taaaaatgaa gggaagattg cccaaggtga
    50581 gtactaattg tgaaataaag cgtggtgatt tatattacgc tgatttaagt ccagttgttg
    50641 gttcagagca gggaggcgtt agacctgttc tgataataca aaatgatgta ggtaataaat
    50701 atagtcctac agttatagta tcagctataa catctcagat taataaagcc aaattaccta
    50761 ctcatgtaga gataagttcc aatgaatatg ggcttaataa agactcagta attcttttag
    50821 agcaaattag aactatcgat aaaaagagac tgagggaaaa aattggttgt ctagatgaaa
    50881 atatgatgat aaaagtagat gatggacttc aaataagttt aggattgttt actttttaaa
    50941 gagggatttg tcttttatta gacaatccct tttttctttt aaatatctct taaggtttaa
    51001 ttttaatgta ctatgttaat aaaattaaaa ataacgcaaa aaaatagcat aaaagtgata
    51061 taataatatg gaattgatat aaaattacga actcaaggga ggtaattgca tgagagacca
    51121 taaaggatta aatgagattg cgcgtatcat aagaagagat atagtttcaa tgatacatag
    51181 agccaaatct ggacatccag gagggtcatt atcagtagtt gaaatactta ctgcgttata
    51241 ctttgatgaa atgaatgtgg attcttcaaa tcctaaaatg gaggatagag atagatttgt
    51301 attatcaaaa ggacatgctg caccagcatt atatgctaca ttggctgaaa aaggctattt
    51361 tgataaggaa gaaatgaatg gattaaggaa gataggaagg atgttacaag gtcatccaga
    51421 catgaaaggc acgccaggtg tggaaatatc tactggttct ttaggtcaag gtttttcagt
    51481 tgcatgtggt atggctatgg catcaaagtt ggataatgct ccgtggaatg tgtatacttt
    51541 gcttggtgat ggtgaggttc aagaaggaat tgtttgggaa gctgctatga gtgctgcaca
    51601 ttataaactt gataatttaa ttgctttttt agataataat ggacttcaaa tagatggaga
    51661 tatagaaagc gtaatgagtt taggttcaat agttgataaa tttaaagctt ttggttggaa
    51721 tgtaattgag atagatggac atgattttga ccaaatattt gcggctcttg atatagctaa
    51781 atcaacagtt aaaaaaccta caatgatagt tgcaaaaact ataaaaggca aaggaatatc
    51841 ttttatggaa aatcaagcag gatggcatgg gacagctcca agtgatgagg agctagaaaa
    51901 agccttgctt gaattaggag gtgttgacaa tgagtaaaat ggcgacgaga gaagcatatg
    51961 gtaaggcatt agtaaaatta ggtcaaataa atgacaatgt tgtagtatta gatgcagatt
    52021 tatcaaagtc tacaaaaaca catgattttt acaagacatt tccagataga ttttttaata
    52081 tgggtatagc tgaacaaaat ttaataggcg ctgcttgtgg attatcaact gctggtaaaa
    52141 taccttttgc aagtactttc gctatgtttg ctacgggaag ggcctttgag attataagaa
    52201 attcagtttg ttatccaaag ctaaatgtaa aaatatgtgc tactcatgca ggtcttactg
    52261 ttggagagga tggagcatct catgaaagtg tagaagatat tgctataatg agagctatac
    52321 caaatatgac agttcttgtt ccagctgatg gagttgaaac agaaaaaatt atttttgaaa
    52381 tagctaagta taatggacct gtttatgtta gattagggag aagttcagta ccagttttat
    52441 ttgatgaaga ttataaattt gaaataggaa aaggtacagt cttaagagaa ggaaaggatg
    52501 tatcaattat agcttgtgga attatggtaa atgaagctct attagcacaa gaaaagttgc
    52561 aagaagaagg tataagtgct agagttataa acatgtcctc gataaaacct atagacaaag
    52621 atttaatatt agaatcagca aaagaaacga atgctattgt aactgtagaa gaacatagca
    52681 taattggtgg attaggttct gctgtaagtg aggtagtggg agagtcttgt ccaaccattg
    52741 taaagaaagt tggaattaaa gatacatttg gagaatctgg taccccaaat gaattgttaa
    52801 aaaaatatga attaacttgt gatgacatag taaagactgt taaagaagct attattgcaa
    52861 aaagaatgta agagatataa attattcatg tttgttaata atataatata tttaaaagag
    52921 aggtataaag tgttatatta atttatatct ctcttttttt tatatgatat caatatatca
    52981 aaaatataat aaaatattca aatttttaca aaaatagaga aaatattcaa atatatattg
    53041 actattcaga aaaatatgac taatatataa tttatagaga aattaatttt atatataatg
    53101 actacttaga taaaataatc taaaaaaatt atgaaaggag gactagggta taaaattaga
    53161 ttataagttt taagttatta tactaaaaag ctataaaaca gttatcatgt attttatgtt
    53221 aaaggaaatt gtaggtagtt tatcgaattg tttaagcaga agaaaaaatt tcacatgggg
    53281 aggtaactga gatatgtcag aaaaagtaaa tgttaaaaca gtcatcagtt tcgcaggtgc
    53341 gtatgtagca acagtaatcg gttctggatt tgcaacagga caggaaataa tgcaattctt
    53401 ctctttttat ggttttgcag gtattattgg tgggataatt tcaatggtat tattctcatg
    53461 gatgggtgct tcagttatgt caaaaggtaa agagcttcaa cttaaagaac ctatcaaaat
    53521 ttatcgtgtt tattgtggta agtatttagg aatattcttt gaatggtttg gacctttatt
    53581 cttatttggt gttttcgtag taatgatttc aggagcagga gcaactttaa ctgaatacta
    53641 tggattaaat ccatttgtag gtagagtagg tatggcagta gtagcattat taactgtttc
    53701 attaggtctt gatagattat caaagatact aggtggaata ggacctataa taataatatt
    53761 tacactttta gttggtggta taagtttagc taaaaatata ggtaatatag gagaagctgc
    53821 acaagttctt agcactgtaa agatagctaa accagttcca aatgcctatc tatctggtgt
    53881 aatatatact acttataaca caattgttgt tatggcattc ttaactggat taggagcaag
    53941 tgctgctaac aaaaaagaag ctatttgtgg tggtatatta ggtggagtag cacttatggc
    54001 tgcagctata atgatgcact tagctattct ttctgatata ggaaacttat attcaaaagc
    54061 aataccatca ttattcttag cagataagat ttcaccaata attggagttt tattctcaat
    54121 aattcttata ttaggtatat atacaacagc tgtaccttta ttatggtcag taactaatag
    54181 atttgtagat gataatcatc ctaagtttaa attaataact ttaatagcag ctatattagg
    54241 tcttataggt ggattcctac catttgataa gctggttggt attttatatc catatactgg
    54301 atatatgggc gtcataatac tggtttgtgt tctttataga cagattacaa aaacatctgg
    54361 atatcaagaa ggaatcagtg acaaataaat attttttaat attgtaaact caagagctat
    54421 atgaaaacaa tgaaaatgtt tttatatagc tctttttatt tttagcttgt ttattatgaa
    54481 atagaaaaaa catgtttttt agttacatta catatattta taattatgta taaatatata
    54541 ctaatcaata atttagagtc taagataagt ttataaatca tatatttaga atatatggga
    54601 ggagtgataa gattgaatgt aaagtttaat attaaaggga taatttatgg agttatagct
    54661 cttgtattcg ttataggggg atttatattc ataccaagta tgtttgtaga gaaatctaaa
    54721 cccatagatt atacagtatt acaaagaaat tcaataccag aaaagatact agatgtgatg
    54781 gataagtata caaatgaaga aagagcatta gcggctaaaa tcgataacaa aatatacatt
    54841 atagtgacta gaggaaataa taaatatggt atagaaatgg acaaaataga aagtgtggtt
    54901 gaagaaggaa aggatgtttt gagggtcaag ataaagtata aagataagga agattctttt
    54961 ccatacatag ttgttgaaac aaatatgagt gaacttccag atagaataga attaaatgca
    55021 acgaaataag aaggtatttt ataattaaat aactgcatat tgtacaataa agtcatggtt
    55081 tttattaatg taagtaaatt gtatatggtt cttattgttg taaaagccat gacttaaaat
    55141 gcttataaag taagatatta acagtagtat ttattactac attaaatggg gtctggaaat
    55201 acttaatatt ctaactattt ggagtgataa ttctagtttc agtaactatg tagtctaatt
    55261 gtgcatcatg agtttctttt ggaatagaat cgaatatctg gaagtcaaat gctatgccga
    55321 tagtaatagc atcttttctt aagttttcta aaaatctatc ataaaatcct ccaccatatc
    55381 ctaatctata gcaatgtata tcatatgcta cagctggtac aattacaaca tctaaggcct
    55441 taatatcaac tgtaggtgat ttttcgttag gttctcttat tccatattct ccaattctca
    55501 atccctcttt taaatctgtt atttgagtag ggattaatgt atgattttct tttattgtga
    55561 tgggacttga tactattttc tttagagaca aaagtttttt gattaaatta tctgtttgaa
    55621 cttcattgtt aaaatttaga taaagcatta tattagttgc atttcttata tcttccattt
    55681 gaagtaattt ttcagtgatt aaatttgaat tactaagtac gaagtttgca ctcttatttt
    55741 ttcttgattc aataactttt tttctaaatt cttttttcat aaaataattc ctcccttaat
    55801 atgttataat gtgtacagca attgtattta atataaattt tagtataatt tattgtaata
    55861 aagcaatata tttgggtaaa tgtaataaaa ataacagcta gtcatataat aataatagtt
    55921 tatgtaaaag gagatgtatc ttgtgatttc aaagaagaaa gctatttttt taggggtgat
    55981 tttagtaatt ataacagcta tggttacatc agcatttcag ttgacattgg ggaataaagt
    56041 tgtaatatcg aaagaactct atgaagacta taaaaaatat gataagttgt taggactaga
    56101 atctatcata aaacaagatt tttataaaaa agtatcagat actgatttgg taaatggagc
    56161 atcaaaaggt ctatttctag gaacaaatga taagtattct ggttattata ctaaagatga
    56221 aatggagaat ctaattaatg atagtgaagg ttcttatgtt ggtgtaggta tgtatatagg
    56281 ggcatcaaaa gatggtggct tagtagtagt tcccatgaaa gattctccag cagagaaagc
    56341 aggtgtaaaa tcaggggata aattagtaaa agtcaatgga aaatctgtct catataaaaa
    56401 ttcagatgaa gctgttcgta tgatgaaggg taaaaaaggt aaaacagttg aactgactat
    56461 attaagagaa gataaacaat tgaactttaa agtaaaaaca gaccagataa ttgagaagag
    56521 tatagaaagt aaagtaattg ataatgattt aggatatata gaaattaccc aatttatatc
    56581 aagtacatat actgactttg ataaagcttt aaaagaatta aaagcaaaaa atatcaaggg
    56641 attagtaata gaccttagaa ataatcctgg tggaatgcta gatatatgta aagaagttgc
    56701 tgatgagtta attggtgaag gaactatagt atatacaaaa gataataagg gaaatactga
    56761 gtacttgaaa tctgataaag aaaaattggg acttccaata gtagtgttaa ctaatggaga
    56821 aagtgcttct gctgctgaaa tattaacagc tgcaatcgtt gataataaag aaggtatttc
    56881 tgtagggact acaacttttg gtaaagggtt ggtacaatca gttgtaagat tgaaggatgg
    56941 aacaggatac aagctaacta ctgctcaata ttttacacct aatggagatt atatcaatga
    57001 aaaaggtata aagcctacta ttgaagagaa agatgaaaat aaacagttag atgtagcaac
    57061 taagtggctt agagaacaga ttgataaata ggatttaatt tttataaact tatactataa
    57121 aataaagaac attataagag agatgtctca aaatgaactt tttagttcat gagggagtct
    57181 ggaaatattt atgtgtattc tttaaataaa aatttatttt ttaagtgata attaaatttt
    57241 tacagacagg gctatggttt taaaaatcat agccttattt tgatattttt taaaaacaca
    57301 ttgcaaccca gttgttccaa atattatatc atttactttt aaaatatcgc aaactgcata
    57361 ttatatattc attataatat aatttatcta actagaacct tagtttacta attaaattag
    57421 atgcatagaa taactttttt aaacattgaa aataaattaa actctaaaca catttaaata
    57481 taaatttata tatcattttg acttggttaa acaatttcat acttatcgat ataaaaatag
    57541 gttataatat aaatgttcat tatcttataa cctattttat ataatttatt cctaaaacat
    57601 gtgaatgttt tttatgatag tttgaagaaa ttataaaatc actttataat tgaatattat
    57661 atataaatta atttaaatat agaaaggaaa atatatgtac aacaatatca aaaatatttt
    57721 aaaagaaaat catataacgt ttgaagaata tgaccatgaa cctattttat catttgaaaa
    57781 agctaaagaa ataagagaaa aattacaata tactggagta gaaagtaaaa gtttatttat
    57841 aaaagatagg tctggaaatt actatatatt tgtaaccaca gaaggaaaaa aattagatcc
    57901 aaaaactata aaaaatgttt taggtggaaa agtatctata tgttcaaaag aagaattaac
    57961 acaaaaaaca caatgtttgc ctggatgtgt atctcctttt ggatatgaga aaaatatctc
    58021 tcttgtaata gatcaagata tatttaacca tgataaaata atcttttcac cagggatacc
    58081 agaaaagaca tttattatta cagctagtgg atttaaaaaa gtaataaaca atataaacaa
    58141 tacaacttat aaatatatat atattaatga attttaatca taattgtaaa ttagagaaaa
    58201 taacattaga aatcatgctt aactatagtg taaataaatt atat
//
LOCUS       DAEMIK010000003        56544 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEMIK010000003 DAEMIK010000000
VERSION     DAEMIK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.003"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..517
                     /locus_tag="KPK87_000147"
     CDS             167..517
                     /locus_tag="KPK87_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG2536351.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            536..2179
                     /locus_tag="KPK87_000148"
     CDS             536..2179
                     /locus_tag="KPK87_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG2536352.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFTKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2260..4317
                     /gene="recG"
                     /locus_tag="KPK87_000149"
     CDS             2260..4317
                     /gene="recG"
                     /locus_tag="KPK87_000149"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG2536353.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4376..4930
                     /gene="rsmD"
                     /locus_tag="KPK87_000150"
     CDS             4376..4930
                     /gene="rsmD"
                     /locus_tag="KPK87_000150"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG2536354.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4932..5429
                     /gene="coaD"
                     /locus_tag="KPK87_000151"
     CDS             4932..5429
                     /gene="coaD"
                     /locus_tag="KPK87_000151"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG2536355.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5433..5957
                     /locus_tag="KPK87_000152"
     CDS             5433..5957
                     /locus_tag="KPK87_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536356.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6301..8220
                     /locus_tag="KPK87_000153"
     CDS             6301..8220
                     /locus_tag="KPK87_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2536357.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8233..8520
                     /locus_tag="KPK87_000154"
     CDS             8233..8520
                     /locus_tag="KPK87_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2536358.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCDVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8653..9912
                     /locus_tag="KPK87_000155"
     CDS             8653..9912
                     /locus_tag="KPK87_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG2536359.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFVGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNIFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9915..10655
                     /locus_tag="KPK87_000156"
     CDS             9915..10655
                     /locus_tag="KPK87_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2536360.1"
                     /translation="MKFKYIDEIRRLIDVIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10978..12288
                     /locus_tag="KPK87_000157"
     CDS             10978..12288
                     /locus_tag="KPK87_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2536361.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12386..13303
                     /locus_tag="KPK87_000158"
     CDS             12386..13303
                     /locus_tag="KPK87_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG2536362.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            13319..14128
                     /locus_tag="KPK87_000159"
     CDS             13319..14128
                     /locus_tag="KPK87_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG2536363.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14186..15934
                     /locus_tag="KPK87_000160"
     CDS             14186..15934
                     /locus_tag="KPK87_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG2536364.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDMLVEQYLPQTFWVEGQVPEDFEENNFNIGIDIFKSFGYEDEEKACTIDVP
                     VEVKNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIIDNYLKELASLGEKV
                     ATIIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16196..17218
                     /locus_tag="KPK87_000161"
     CDS             16196..17218
                     /locus_tag="KPK87_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG2536365.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17223..19529
                     /locus_tag="KPK87_000162"
     CDS             17223..19529
                     /locus_tag="KPK87_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2536366.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19501..20616
                     /locus_tag="KPK87_000163"
     CDS             19501..20616
                     /locus_tag="KPK87_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536367.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVRIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVLGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20634..22052
                     /locus_tag="KPK87_000164"
     CDS             20634..22052
                     /locus_tag="KPK87_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG2536368.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22067..23275
                     /locus_tag="KPK87_000165"
     CDS             22067..23275
                     /locus_tag="KPK87_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536369.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(23356..24744)
                     /locus_tag="KPK87_000166"
     CDS             complement(23356..24744)
                     /locus_tag="KPK87_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG2536370.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEIFSKKNIS"
     gene            25085..26419
                     /locus_tag="KPK87_000167"
     CDS             25085..26419
                     /locus_tag="KPK87_000167"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG2536371.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26548..27465)
                     /locus_tag="KPK87_000168"
     CDS             complement(26548..27465)
                     /locus_tag="KPK87_000168"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG2536372.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27673..28557
                     /locus_tag="KPK87_000169"
     CDS             27673..28557
                     /locus_tag="KPK87_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG2536373.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28770..30611
                     /locus_tag="KPK87_000170"
     CDS             28770..30611
                     /locus_tag="KPK87_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG2536374.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFNNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30916..31623
                     /locus_tag="KPK87_000171"
     CDS             30916..31623
                     /locus_tag="KPK87_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536375.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31617..32927
                     /locus_tag="KPK87_000172"
     CDS             31617..32927
                     /locus_tag="KPK87_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2536376.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33044..33727
                     /locus_tag="KPK87_000173"
     CDS             33044..33727
                     /locus_tag="KPK87_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536377.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVMLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VLKVGDKND"
     gene            33720..36107
                     /locus_tag="KPK87_000174"
     CDS             33720..36107
                     /locus_tag="KPK87_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2536378.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36387..37529
                     /locus_tag="KPK87_000175"
     CDS             36387..37529
                     /locus_tag="KPK87_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2536379.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37548..38072
                     /locus_tag="KPK87_000176"
     CDS             37548..38072
                     /locus_tag="KPK87_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2536380.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38172..38990
                     /locus_tag="KPK87_000177"
     CDS             38172..38990
                     /locus_tag="KPK87_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2536381.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPIQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            39068..39379
                     /locus_tag="KPK87_000178"
     CDS             39068..39379
                     /locus_tag="KPK87_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG2536382.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39506..41509)
                     /locus_tag="KPK87_000179"
     CDS             complement(39506..41509)
                     /locus_tag="KPK87_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG2536383.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMEHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(41662..42300)
                     /gene="recX"
                     /locus_tag="KPK87_000180"
     CDS             complement(41662..42300)
                     /gene="recX"
                     /locus_tag="KPK87_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG2536384.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            42576..43265
                     /locus_tag="KPK87_000181"
     CDS             42576..43265
                     /locus_tag="KPK87_000181"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG2536385.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            43266..43838
                     /gene="yqeK"
                     /locus_tag="KPK87_000182"
     CDS             43266..43838
                     /gene="yqeK"
                     /locus_tag="KPK87_000182"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG2536386.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINSIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            43955..44305
                     /gene="rsfS"
                     /locus_tag="KPK87_000183"
     CDS             43955..44305
                     /gene="rsfS"
                     /locus_tag="KPK87_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG2536387.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            44659..47079
                     /locus_tag="KPK87_000184"
     CDS             44659..47079
                     /locus_tag="KPK87_000184"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG2536388.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            47914..48582
                     /locus_tag="KPK87_000185"
     CDS             47914..48582
                     /locus_tag="KPK87_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2536389.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(48900..49172)
                     /locus_tag="KPK87_000186"
     CDS             complement(48900..49172)
                     /locus_tag="KPK87_000186"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2536390.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(49403..49824)
                     /locus_tag="KPK87_000187"
                     /pseudo
     CDS             complement(49403..49824)
                     /locus_tag="KPK87_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(49843..49941)
                     /locus_tag="KPK87_000188"
                     /pseudo
     CDS             complement(49843..49941)
                     /locus_tag="KPK87_000188"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            50224..51594
                     /locus_tag="KPK87_000189"
     CDS             50224..51594
                     /locus_tag="KPK87_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG2536391.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            51953..52111
                     /locus_tag="KPK87_000190"
     CDS             51953..52111
                     /locus_tag="KPK87_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536392.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   52397..52688
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=52397..52425
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     regulatory      complement(52830..52927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            53153..53536
                     /locus_tag="KPK87_000191"
                     /pseudo
     CDS             53153..53536
                     /locus_tag="KPK87_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(53725..54720)
                     /locus_tag="KPK87_000192"
     CDS             complement(53725..54720)
                     /locus_tag="KPK87_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG2536393.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(54841..56469)
                     /gene="aspD"
                     /locus_tag="KPK87_000193"
     CDS             complement(54841..56469)
                     /gene="aspD"
                     /locus_tag="KPK87_000193"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG2536394.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFIGKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT        21909 a         6346 c         9618 g        18671 t
ORIGIN      
        1 gaatatatat actatagaag aaattttatc caaaaacttt tctaggttta aattatacca
       61 tatttatagt atgatatgat tataatttta tataataaat aagtaaaaat agtaaaaaat
      121 aaattaagaa atgtttttaa taaaaataag cttaaggggg tcaaccatga gtgcaaaggt
      181 aacgaatcaa tatggaaata tagaaataga taatcaagta atagctcaag tcgtatatag
      241 agctgcaatg gaaagttatg gcttagtagg tttagcatat aagtctaaag gaatagtaga
      301 gctattaaaa ggcgagaacg caactaaagg tgtaagagtt gaggaagtag aagaagatgc
      361 catagcaatc gaactttatg ttataataca atatggtaca aatatatcta ctgttgccaa
      421 taatataata gatagagtaa aatatgtagt tgaaaaaatg actggcgtaa gagttactag
      481 aattgatatt aatgtacaag gaataagagt taagtaattt cataggagga aacaaatgat
      541 tcaatatata gatggtaaaa ggttaaggga gatgtttatt tcaggtgcaa acaatctcca
      601 aaacaataaa gagttagttg acaagttaaa tgtattccct gtaccagatg gagatacagg
      661 aactaatatg tcattaacaa tatcttatgc cttaaaagaa ttagcaaaag ttgaaaatga
      721 taatatatca gatatcggaa aagcattatc taaaggttct ttaatgggag ctagaggaaa
      781 ctcaggagtt atactttcac aaataatcag aggtatagct aaatctatag aaggaaaaag
      841 taaattatca acagaagatt tagcaaaagc ttttaaaaat ggttcagaca cagcatataa
      901 agctgtaata aaaccaattg aaggaactat acttactgta gttagagaaa gtggagagtt
      961 tgcaataaaa actgcgaaaa aagagaaaga tgtagtaaag tttttaagta tgcttgtgaa
     1021 ggaatcaaat tcttcattag aaagaactcc agaccttcta aagaatctaa aagaagctgg
     1081 tgtggttgac tctggtggaa agggtcttgt acttatatat gaaggaatgt tagcttctat
     1141 aaaaggcgat aacatagaaa taaaaaatgt tgacctagat actaatattt caacaagtat
     1201 ggattttaca aagagtacta ctagcacaga tgatataaaa tattgctatt gtacagaatt
     1261 tattttagaa agttctaagg tagaagatac taaaataaga gacataatga tggcttatgg
     1321 tgatagccta gcagtagttg gagatgatgg agtcataaag gtacatgtac acactaatga
     1381 cccaggaaat gtacttcaag aagctctaaa gtacggtcaa ttattaacta taaaaataga
     1441 aaatatgaag ttacaacatg aaaatacatt actggatgta gaagaaaaga aagaaaatga
     1501 tagtgaacct ttagaagaag aaaaagagtt tgggtttata gctacttcta tgggtgaagg
     1561 tttggcaaat atatttaaag atttcggagt tgaccatata atagaaggtg gtcaaactat
     1621 gaatccaagt actgaagatt ttatgaatgc tataaagcat ataaatgcta aaaatatatt
     1681 tattttccca aataacagca atataataat ggctgctaat caggcaaaag aattgagtga
     1741 taaaaatata atagttatac ctacaaaaaa tacgcctcaa ggatttgctg cattagtaac
     1801 ttttaatgga gaattaagcg aagatgaaaa taaagaagct atgatgaatg ctttaaactc
     1861 agttaaatca ggtcaagtaa cttttgctgt tagggataca gtgatgaacg aaattgatgt
     1921 taaagaaggt aatataattg gaattgcaga agggaattta ttatcagcag gagattatgt
     1981 ggatgaagta acatcaaacc ttattgaaaa gttggttgat gaagatactg ctataataac
     2041 attattcttt ggtgaagatg ttacagaatc tcaagcaaat gaactacgta cttctttaga
     2101 agaaaagttt gaagatgtag atgtagagtt atattatgga ggacaacctc tttattatta
     2161 cttaatttca gttgaataat gattataaga acctgtatga ccaagtagct tttataaaat
     2221 tggttgtaca ggatttttgt gtagtaggtg atatatttgt tggatttata taaagatgtt
     2281 caatatgtta agggaatagg acctaaaaaa gcagataaat taaataaact gggtatattt
     2341 acactaaaag acctattgta ttattttcca agacaatttg aagatagaaa taatctaaag
     2401 aaaatagcac aattagaaga tggtgagaaa gttacgataa aagcagtaat atcaagtata
     2461 aatacattta gcccaaaaga aggtatgact ttaacaaaga ttgatgtaaa agatgagaca
     2521 ggctcggcta aattagtgtt ttttaataag agttatataa aaaatacttt tcgtccagga
     2581 gattcaatat tagtttttgg gaaggttaag aaaaaattta ataacttaga acttacatca
     2641 tgtgaattag aatatcttac taattcacct aagaatacat gtagatttat gcctgtgtac
     2701 cagcttacat acggtgttac aaataaagaa attatgagta taattagaac agtacttgaa
     2761 gataaagaat taattataca agaatatatg ccacaaagaa ttatagaaaa atataggcta
     2821 tgtagtatag attttgcagt tagaaacata cattcaccaa gtagtaagga atcattaaaa
     2881 atagctttat atagaattgt atttgaagaa ttacttatac tacaactagg gttgtttgta
     2941 tttaaaagtg gcagaaataa agaagatgga ataaagtttg aaacaagtaa agacttaaaa
     3001 aaaataataa gtgctttacc atttaaactg actaaagctc aaaacagggc attagatgaa
     3061 attatacaag atatgaattt agagaaaata atgaatagat tggtacaagg tgatgtaggg
     3121 agtggtaaaa cagtagtagc tctcttagca cttgcaaatt gtgttttaaa tggttatcag
     3181 ggcgctttga tggcacctac agagatatta gcagggcaac attatatttc acttactgag
     3241 tctctaaaag attttggaat aaatgtaggt ctattaatag gtagtcttac aaaaaaacaa
     3301 aaggatacag tattggaaca aataaagaat aatgagattg atatattaat aggtacacat
     3361 gcactaattg aagataaggt tgaatttaat aatattggtt tagttataac agatgaacaa
     3421 catagatttg gtgttatgca aaggagcaaa ctttcattga aaggagcaaa tcctgatata
     3481 ttagtaatga cagcaacacc aataccaaga actttagcac ttatactcta tggagattta
     3541 gatatttcta ttatagatga acttcctcca ggaagacagc caatagaaac aattgctatt
     3601 gaaaaaagta agcgagatag agcatataat aatcttgtaa gaagagaggt tgaatcagga
     3661 agacaagtgt acattgtatg tcccttggta gaagaaagtg aagccataga agccaaatca
     3721 gcagttgaac ttgttgagga gttaagagcg gaatattttc acgacttaag gcttgggttg
     3781 cttcatggta aaatgaagtc aagtgaaaaa gatgaagtaa tgagactttt taaaaataaa
     3841 gaaatagata ttttagtttc aacaacagta atagaagttg gtgtcaatgt gccaaatgca
     3901 acattaatga taatagaaaa tgcagaaaga tttggacttg cacaattaca tcagcttaga
     3961 gggcgagttg gaagaggtag tcataaatcg tattgtgtac taatttatga ttctaaaaca
     4021 gatgtatgta gacaaagaat ggctataatg gaagagacca atgatggttt taagatttca
     4081 gagaaggatt tagaaataag aggacctggg gaattttttg ggacaaggca gcatggtctt
     4141 ccagaactta aggtagcaaa cttatttaaa catataaaaa tactgaagtt agcacagcaa
     4201 gaagctcgat atatattggg tgaagataat aatctacaat taaaagaaaa catggcactc
     4261 aaaaaagaaa ttatagataa atttaaagat acattgaaag aaatttcatt aaattaattt
     4321 taaattgtgt taaaatatta ctaatgtaat aatgtgctga agggagattg tcatattgag
     4381 agtaatttca ggaaaagcga gaggattaaa actaaatact ccaaaaaatg aagatgtaag
     4441 gccaactaca gatagagtaa aagagtctct atttaatatg ataaattcat atataatgga
     4501 aagtgaagtt ctggatttat ttgctgggac tggttcttta ggaatagagt gcttatctag
     4561 gggtgccaag tcatgtactt ttgtagatat cagtaaggaa agcatagata ttgttaagtc
     4621 caatataaaa aaggcaagag ttgaaagtga aagtattatt ttaaaccttg attttaagac
     4681 tgcaatagat aaacttaaat tacaaaatag taaatttgac ataattttta tggaccctcc
     4741 gtattataaa aatatgttta tagaagctat tgaaaaaatt gataattcta atctattgaa
     4801 cgaagatgga ataatagttg tagaacatga tacgaatgat ttatttccag ataagatatg
     4861 taaattggag aaaactagag ataagaaata tggaaatact acattaactt tttataagat
     4921 ggaggcataa tatggaaaat aaacctagaa aagctatatt tgcaggaagt tttgacccaa
     4981 ttactaatgg acatttagat attatttgca gggcttcgaa gttatttgac gaattacaaa
     5041 taggagtatt aaataatcct aataaaaagg gtctatttag ctttgatgaa agagtaaaac
     5101 ttatagaaaa aagtacaagc catttaaata atataaaagt tgtgactttt gatggtttat
     5161 taataaatta ctgtcaggaa aatgggattg gagcattagt tagaggtgtg agaagtggtg
     5221 ctgatgttga ttatgaactt cagatggcgc atatgaatag agagcttaat ccagatatag
     5281 agactataat tttaccaagt tgtacaaaat attcatttat aagttcatct ttaataaaag
     5341 aggttttgct ctttgatgct gatataaaaa atttggttcc aaagattgtt ttggaagaat
     5401 taaagaaaaa aactagtggg ggtaattgat atatgaagat agatttagaa atgattgaac
     5461 tatttgagca gttagaggag atatttagaa gtgcttctac aatccctttc tcacataaat
     5521 gcacagtgga taaagatgag gttttagcaa taattaatga tataagaact ttaataccag
     5581 aagaagtaac acaggcagta tggattaata aagaaagaaa taaaataata agtcaagcta
     5641 agcaagatgc tacaaatata gttgaacaag cgaaaaaaga agctgagaga atacaacaag
     5701 aatatcaaga taatatagag gaattaaaga aaaattctga agatgtagta aaggcatatg
     5761 tagaatccag tgaacctgtt gttcaagcag accaaagagc aaaagatata gttgatagag
     5821 ctgagagaat agccaatgag ataagaattg ggtcaattga atatgcagag gatgtgcttt
     5881 ctagtgttga gtataattta agagaaatat tagaagaagt aaaaagagat aaagctgaac
     5941 ttagacctaa aaaataaatt ttaaataaat aaaaatggag ctatcacaca aaaaattcca
     6001 atttatgtgt gatagctctc ttttaatata gcaatttttt gttatataat tgtgcttttt
     6061 agattactta tatcttattt tgatattata atgtagttta atcttttatt tgttgaaatc
     6121 aacttattta tttgtataaa taatataggt tattttcaaa tttttttaat aattttacca
     6181 ctgtcttctt ttagattaga gttagactgt gcaaaattat aaaataatac taaatcatgt
     6241 gcaattattt ggtatggcac tacatgtaga aaatatagat aataaaatta aggaggtagt
     6301 atgaaggaaa gggctaaaaa aatattagat tttattatta aaaataacaa tgtaacatca
     6361 caagaactac aagaaaagtt taatgtttca aagagaacta tttattacga tattctagct
     6421 ataaatgaac aactaggaaa aagtggtaat ataaaaaatg taaaacacaa atttatattt
     6481 gaaggtaatt tatgtgatgc aaggaaaata atttcaactg aagaagataa atttttagat
     6541 agtgactaca gaaaaacttt tatattaaac aagatattac ttggcgaaaa aatatcaata
     6601 gaaaaactaa caaatgaaat gttactttca aaaaacacag tagtacaaac aataactgat
     6661 gtaaaaaaat accttcaaac aatggggtta agattagagt ataagggaaa gtacaaaatt
     6721 ataggtgatg aatacgttat tagagagctg tttctgataa ttgttcaaga aaatgtactg
     6781 gaaattaatt ctataagtga ggaagtaagt agtttcgata caaaaggaca tataaaactt
     6841 actgattatt ctttattaaa tctaactaag tttgtggaat ttctaaataa aaggataaga
     6901 gatgggaaaa cattatactc atataaatat ctaaatgaag ctaaaaaaat tagttacttt
     6961 agtaattgta aagaactttt atgtgaagaa gctaatgaaa atgaacaagc ctatatatgc
     7021 acttacataa gctcacttcc cagcttaaat agtgaagtta aggaagatgt tgttgaagaa
     7081 tatgtagata aacttataga taagtttgaa gtaaatacag caataaaatt agaaagtaag
     7141 catgagttta agaaaaatat tttaaggcac ctccactctt cttacaacag aatacgattt
     7201 aagtttccta ttagaaatcc aatgttagat gagactaagt ataaacatga gtcactttat
     7261 aaaattataa aatcaattat agagaatgaa gaagagtttc ctgtatttga ggggataaga
     7321 gaagaagaaa taggatttat agcagcgtat tttggaggat atttaagagg gtctagggat
     7381 aatggtctaa gaagaaataa agtcttacta gtttgtccta atggtcttat ggtatcaaag
     7441 agtttggaaa tacaactata taaatacata ccaaccatag aaatagtagg tatagtatcc
     7501 attaagcaac taaaagaagt aaatgtatac tacgattaca taattacaac tatagatata
     7561 caaaatgtaa acaacgtaat tgttgtaaat cctcttttaa catcatcaga tgttcaatta
     7621 cttatgaaca aactaataag tgtaaaagaa aacgagaagt atttcaatct tgaattaata
     7681 atacaagcta taagaaaaaa tggagttatc aataatgaag aagcactaaa agctgattta
     7741 ttaaacataa tacacaagat agatgaagga gagatgtatc aaccaatgtt aaaggaatta
     7801 attaatgcag aaagggtcaa tataataaaa aatgttagag attggaaaga agcaataaaa
     7861 atagcctcaa agccattgtt agaagataac tctatagaag agttatatat agaaaatatg
     7921 ataaagtcag ttgagaaata tggaccatat attgttttag ctgatagatt tgctctacca
     7981 catgcaagtt ctaaagaagg agtaaacaag cttgctatgt ctttactaat agtagaagat
     8041 gaagttgatt tattaggaaa accagtaaat atatttatgg tattagctgc tgtagataac
     8101 actactcaca ttagagcatt agcatcacta tcagagatga tgtacgagga agaaaatgta
     8161 aaactaataa ttaatggtga taaaagttca atactagaac ttataaataa gcaaaattag
     8221 gaggaatgaa aaatgaagat attagcagta tgtggttttg gtgtaggttc atcaatggta
     8281 ttaaaaatga caatacaaaa ggctctaaag cagttaggta tagaatgtga agtagaaaat
     8341 acagatataa attcagctag aggtgctgat tgtgactgta tattcacaag tcaagaattg
     8401 ttaaatgaat taaaaggaac ttgtgatgta gatgtatatc cagttaaaaa gtacatggat
     8461 ttagcagaag tcacagaagt tgttcaaact tttttaagtg ataaaaatga taagaaatag
     8521 tataagtttt aaaaagtaaa tttaagttaa tcagaataaa ataaaaactt actaaattca
     8581 aaaatagcaa gaaagagagg agggatttag tggtagaaaa cttaaacagt tgacctatca
     8641 ctaaaaaata taatgcttga ttttatttca aataatattt tacgtaatcc atctatgtta
     8701 attggattaa tagcagcatt tggtcttgta ctacagaaga agaaagcatc tgatgttata
     8761 aaggggtcac tacttgcagc tttcggaatt ataattatgg agactggaac aggtatgtta
     8821 gttagttcaa tagcaccaat taatggagct ttccaaagta taagtggtgg tgcagtaaca
     8881 aaaggtttaa gtgatgttac ttttactgca agttatggtg gaattatagg tttagcaatg
     8941 tttgtgggtt tagctattca cctaatgata gcaagattta ctcctattaa gacaatcttc
     9001 ttaacaggtc acatgcttta ttggttccca ttcatatttg tagcttcagg agttgaagct
     9061 ggattaaatg gtggagtatt aatagctttt gcagcagtat tatcagcact ctattggtca
     9121 ataatgcctt ggttattaag aaaatatgtg tttgatgtta taggagatga ttcttttaca
     9181 ttaggtcatc catctggttg tttagcactt atatcaggat ttgttgctaa attagtagga
     9241 aataaagaaa aatctacaga agatataaac atacctcaaa gtttatcatt ttttagagaa
     9301 atatctataa gtggtggaat ggcagtgttt ttaatgttta tagttgttgg gatagtaata
     9361 ccaggtgcgt ttaaagatag taatgagttt attggaacgt atgcaataaa gcaaggtctt
     9421 tcttttggag caggtcttat aataatgctt catggggtaa gaatgctaat aaatcaaata
     9481 atgccagcat ttcaaggtat atctgaaaaa gttgtgccaa atgcactacc tgcttttgac
     9541 tgtccaatat tatttaatta taaaccaaat gctgtattaa taggattttt agttgctatg
     9601 ctaacatcta cagctatgat tttaatatgt aactacttta atatatttaa tttgctactt
     9661 ataccacttg ttataacttc attctttgag tgtggagcag cagcagtaat aggagaatca
     9721 caaggtggta tacgtggagc tataataggt acagctatag ctgcaatatt tatggtagtt
     9781 ttagtaggaa tatctgctgt aatttactca ggaactattc aaaattggat actaatattt
     9841 ggaggtaatg acctttcatt atttggtata atcagtaggt ttataggttc actatttgga
     9901 ggaatattat agatatgaaa tttaaataca tagatgagat aagaagatta attgatgtaa
     9961 tagaaaacga agaaaaagtc aatatggaaa aagcagtaca aatccttgtt gaagcaatag
    10021 aaagtaaaaa tgctatattt tcatttggag cttcacatgc aggaatatta acagaagaat
    10081 tattctatag agcaggtggc tgtgttctta taaatccagt atttgcaaaa tctctaatgt
    10141 tagatacagc acctataact cacacaagta agatggaaag attagaagga tatggtacag
    10201 ctctagctga taaaactcca attaaaaaag gagatgtagt tatagttcat tcagtatctg
    10261 gtagaaatcc agtaagtata gagtttgcta tgaaagctaa ggaaaataag gcaatagtaa
    10321 tatgtataac taatttaagt tactcaaaaa gtgtatcttc aagacattct tcaggaaaaa
    10381 aactttatga agtatgcgat ttagtgatag ataatcatgg tcaaaaggga gatgcctgtg
    10441 tagagataga tggattaaac caaaaagtta gtccaacttc tactgtaata gcaactactg
    10501 tgatgaactc tattatagct caagctactc aggaattagt taataaaggg ctaaagaatc
    10561 ctccgatttt ctatagtgcc aatattgatg gtggagatga gttaaataag aagatttttg
    10621 atgaatataa aagtgtaatt cattatgaat attaaggtta aaaaaatatg ttaatgaact
    10681 ttgaagttta tcaacaaaga atattaataa tatataataa atgctatcat ttaaaagctg
    10741 taggtaaaaa tctacagctt tttattattg tagattaaaa agatgcgtaa ttaaatttta
    10801 aattggagaa aataaattgg aaggtatcaa ttataaatat tagaaacgtt gtcatagaaa
    10861 aaaactatat taaaaaaaag tacatagaaa aaactgatta gacaataaat acatgctttg
    10921 ataatatttt aaatggagtg aaaatgtgta gaattataat gaaaaaagaa aataaaaatg
    10981 aaaaaagaaa ataaaatgga gggaataaaa gtgggtatgt taaaaaaatt atgttcacta
    11041 gctatggtag caattatgac tacaacactt ataacagggt gttcaagtgg aaatggaaaa
    11101 gattcaaaaa aggatggcga aaaagaaaaa ctagttcttt ggatgccacc agtagaaaat
    11161 aatatgaagg aagtttggga ccctatttta gataaatttg aggaaaaaaa taactgtgaa
    11221 gtagatttac agataattcc ttgggataat tattctgaaa aatttgcaac agctataagt
    11281 gctggcgaag gcccagatgt aggatatatg tatgcagaaa tgtatccaca gtttatagaa
    11341 agtggagcag tagaagattt gactaactat gcaactaaag aggataaaga gcaatctatt
    11401 tatataaaaa catctgaaat gatgggtgga atgtatggta tgccattcca agcagcaaat
    11461 ccaggggtat tatactataa caaggatatt ttagaaaaat taggagaaaa acctcctaaa
    11521 acatgggaag attttaaaag aatttgtaaa aaagcaacac aagatactga tggtgatgga
    11581 aagatagacc aatggggatt agctcaggga tggggagcta aaacttttgg taacttaaac
    11641 tggaactggt atccttattt atggcaagct ggtggagata tctttaatga tgatttaaaa
    11701 tcggttaaat ttaatgataa gagtggttta gaagcagcta atttcttaaa agaattacaa
    11761 gcatatgtgc ctgaagattc tttatctaaa gatagtaatg aaatgataga aagtgtattt
    11821 ggaccaggga aagcagcatt tacaataatg ttatcttctg ctgcaacaag tgtatttgat
    11881 gaatcattcc cggatttaaa ttggggattt gtaactggac tagaagataa gaaagcagca
    11941 acttttggag cagtagacca tttatcttta atgtcatctg cgaaaaataa agaacttgca
    12001 tacaagttaa tacaacatat gttaagtgtt gaatcaatga cagaatttca taaagcaata
    12061 ccaagagcac caataacaaa aggtgaacca tatcaaggtg atgaaagatt caaagaaatg
    12121 gtggaaaatg acaaaaatgt atatagacca ttagtagtag gacctcatgg agttgaaata
    12181 tatgaatatt tatggaaaga acttcaaact atgatttcag gagataaaac tccaaaacaa
    12241 gcgttagatg atgctgcaaa atattcaaat gatttattag cacaataaaa gtttaaagct
    12301 gtaatatgtt gtaaaggact ttacagatta aaattagcat tgtatttata aatacaatgc
    12361 taatatttat attggagagg ataaaatgga aaacttagtt agagagtttg aagaaaattc
    12421 aaaaagaaaa aaaacaaaat taaatattaa accatatatg tatatattgc cattgggaat
    12481 aattttagtg gcgttttatg tgataccaat aataatgtca atatacttta gttttacaaa
    12541 gtataatatt attagtccag ctacttttat aggtttagaa aattataaaa aattatttac
    12601 agatgaaaca ttaaaagtat ctattataaa tactattaaa tttactgttg ttgtagtacc
    12661 ttgccaaact atattatcat tgattttggc tgtttggatt acaggaaaag gaaatagtaa
    12721 aatagcttca tttgctaaag gtgctatatt tataccagta ttatcatcta tggttttgat
    12781 aggtatggta tggagggctt tattgaatgg agaaggttct attatatatc aggtattagg
    12841 tgcctttgga atagaatcat ctaaactttt aggagatagt aagacagcat tacctacact
    12901 tatgtttata tctatgtgga aaaatatagg gtatttcatg gtaatttata tttcagctat
    12961 aatgaattta ccaaaacact gctatgaagt agcgaaggtt gatggggcta caaaacttca
    13021 agaatttata aaaataactg tacctttatt gaaaccaact acaataatgg ttgtattttt
    13081 agggtcgata tggtcattac aagtatttga cttaatttat actgtgacag gtggaggtcc
    13141 aggtatatct actatgagta tagttatgca tgcatttaac ttaaacttta aaaactttaa
    13201 ttctggatat gcaatgactg tagcaaacgt attatttttg ttaatagcag tagtttcgat
    13261 tttacaaaat aagcttgtaa gacgagacaa ttcagatttt tagaaaggta ggtataatat
    13321 gaaaacagca agaagattat taagtgatgg tttactttta tttatagcgt gtgcaagttt
    13381 ggtacctttt atatacatgt tgataatatc attgaagata acatataact catatagttt
    13441 ggatatatct ttttcaacag taacccttca aaactatata gatattttta ctaaaaaagg
    13501 atttgcacaa tattttttta atacagcaat agtatctttt tcaggtgtat tattaaattt
    13561 agtatttagt acattagcag gatattcttt tgcgaagatg gattttaaag gtagtgataa
    13621 gttattttta tttatgataa tgactttaat tataccttcc caagttacaa tgataccact
    13681 atatataata atgaaacact taggatggat aaatagttat cttgcactta ttatgccaat
    13741 accaactgca tttggcgtat ttataatgag acaagctatt ttaggagtac caaaagagtt
    13801 attagaatca gcaaagatag atggttgctc ggattttaga attttaatac aaatagtgtt
    13861 gcctttaatt aaaccagcat taattacact agcaatattt acttttatgg gagcatggaa
    13921 cgagtttatg tggcctctta tagctacaac taaagatgca atgagaacat taacagttgg
    13981 attatctact cttaagacat tccagataac taattatgga cagatgatgg caggggctac
    14041 aataacattt ttacctccat ttattttcta tttgatttta cagagtaaat ttgttgaagg
    14101 tgtgtctttg tctggtataa aaggttaaat agtctaattt aataatatat aaagaaactt
    14161 atataaatat ggaggaattt aaactatgga aatttcatat gtactgaaag attgcacatt
    14221 caaacacaat aaatactcag ttgtagaagc tgaaacttgg aaagataagg atttggatat
    14281 tgtagtagct aaaggtgaaa aatttgcatt tcaaattatg ttaaaagcaa cagaagaatt
    14341 taattgtaca attgataaaa gtagtgctat atcatggaaa ggcctgggaa atagagtaag
    14401 attagcatta aatgtaccta atagtttaga aaataatttt agtataaata tactaggata
    14461 tgttcaagat gatacaaaag catttgtgaa tgatgctatt ttaagggata aagatatgtt
    14521 agtagagcaa tacttgcctc aaacattttg ggtcgaaggt caagttccgg aagattttga
    14581 agaaaataac tttaatatag gtattgatat atttaaaagc tttggatatg aagatgaaga
    14641 aaaagcttgt acaattgacg ttcctgtaga agtaaaaaat gttgtattaa agcctttaga
    14701 tgaaagtaag ttctttttag atttatggca acatccaagc tgtctagcta gaatgtataa
    14761 agttgagcta tggtctgata tacattttga aataatcgat aactatctaa aagaattagc
    14821 ttctttagga gaaaaagttg caacaattat agtttcagat tatccatggg cagggcaaag
    14881 ttgttataaa gtttataaaa atccatcaaa cttatatgaa tataacatgg taagtgtatc
    14941 aaaaggattg gatggaaaga ttaaatgtaa ctttgagagt atggataggt atataagcat
    15001 agctgataag tataaaatgg ctaaggagat agatttattt ggtctattag gtaattggtg
    15061 tgctggtgag tttggaaatc ctgtagaagg ttataaggac cctataagag taagatattt
    15121 tgatgaaaag gataaagtat ttaaatttat aaacaataca aatgatttaa aagaatatat
    15181 aggattagta ttaaatcatt taatagagtg tggattatgg gacagagtaa ggattatagc
    15241 tgatgaacca aataatccag aagtagtcaa agagtgtata gaatttataa attctactgt
    15301 aggtactcat caagttaaat ataaatcagc aactcacgac caaaactttt tagacagagc
    15361 taaagatgaa attgatgata tgtcaataaa tttaaaactt actatacaaa attataaaga
    15421 tatagaaagt cttaagaaaa aaatcaatga caagggtggg atattgactt ggtttgtatg
    15481 ctgttttcca gagaaaccaa atagtttttt aagttctcct tttgtagaaa atagaataat
    15541 aggatggtat acttattact ttggtcttga tggattttta agatgggact ataatttatg
    15601 gacagaagac ccttggaaag attcaagtta caaattccct atttggaaag ctggagatat
    15661 gttctttgtt tatcctggaa aagatttaaa gccagttaga agtgtcagaa tggagaattt
    15721 aagatttgga atacaggact ttgagttatt tacaatgtta gaaaaagaaa agggaagaga
    15781 gtatatagaa gttgagctta tgcaagaatt attaaataaa aaagaaaatg ctgaaattaa
    15841 aggttttggt gacatagaac taggatattc acttgataat tggggatatg ataaagttaa
    15901 aaaatatgta ttagagttgt tagacagagt ataaaaaata taagagtgtc tcaaaatgaa
    15961 ctttttagtt catgaggcac tctttttgta tgaaatcaat atagtttcat tgtttcaaca
    16021 atgaaaaata ggattatttc tattctaaga cagcccttta gttttttagt tgtattcaat
    16081 gttttaaaac taactataaa cgctataaag tttataaatg acaataatta tatataacat
    16141 gatgtacttt aatttgctat aatagggatt gaataatatt atatggaaga taacattgtt
    16201 tggggggatt aatataaaaa gaataggtat tattataatg ttttttttaa gtatatcttt
    16261 tattggattt tatcttgtaa taagagataa cagtgataca gttaagatgc aacgagggat
    16321 aaatataggt aatgcactag aatcacctaa ggattttccg tgggatgtaa aaatgtcaaa
    16381 taagtttttt gatgatataa aagatgcagg ttttgacaca gtacgtatac cagtaaggtt
    16441 ttctgattat acgtcagatt ctgataattt taaaattgat gaagaatttt ttaaaaagat
    16501 tgataaatat gtagattatg cattggataa agacttaata gttgtgttgg atttacacca
    16561 ttttgaagaa attatgaaag aaccaagagt tcataaagaa aagtttttaa aaatatggca
    16621 acagatagct aataggtatc aaaaatatga taaaaaattg gtatttgaat tattaaatga
    16681 acctaaagaa aatttatatt cacagttact aaacgaatac attgaagaag caataaagat
    16741 aattagaaaa actaacccca aaagaactat tatagtagga ccatataatt tctatcaaat
    16801 tgattatttg aatgaattaa atattcctaa agattctaat atagtagttt cttttcacta
    16861 ttacgagcca aatgattttg catttcaagg aaatatctat cataagggat tcgagcactt
    16921 aagcaatatt acttgggaag gaacaaatga acaaatggat tatttaaaaa aaaggtttga
    16981 tactgtggaa aattgggcaa ataaaaataa ggtaaaaatc ttcttgggtg aatttggaat
    17041 aacaaaagag gcaccagaag cttcaagaag agcatggata aaagctgtaa gagaagaagc
    17101 tgaaaaacga aacttttcat gggcttattg ggagcttgca tctggatttg gtatatacaa
    17161 tcaaattgaa gggacttggg atagagatat attaagtgca ttaatagaaa agaggtgatt
    17221 ttatgaagaa aaattcaaaa aagatactta taactttaac tattattact aatgtcatat
    17281 atatattgtg gagaattttt tacacagtac ctaaagaaga agggaagttt gccttaatat
    17341 gtgccataat acttcttttt gtagagataa tgggcatgat ggaaatgttt gtgcattatt
    17401 atggtatgtc aaatattgag tatcctgaaa aaccaatcat aagtgaagaa ctttatccac
    17461 atgtagatgt atttattgca acatataatg aatctgttga tttggttaga aaaactgtaa
    17521 atggatgtat tcatatgcag tatccagata agaaaaaggt acatatatac atatgtgatg
    17581 atggaaatcg tgaagaaatg cgtattctag cagaaaaaat gggtgtaaat tacataacta
    17641 gaactgaaag agaaggagca aaagctggaa atttaaataa tgcaatgcaa catacaaatt
    17701 cccctttgat tgctacattt gatgcagata tgataccaat gcatgacttt ttaattgcta
    17761 cagtaccata ctttttgaaa aatgagcaag ctaaaaaaga tggagaaaaa gaggagtatg
    17821 aaaaggttgg ttttgtgcaa actccacaga gtttttataa tccagatttg ttccaattta
    17881 atctgcactc tgaaggaagg attccaaatg agcaagatta tttttacaga gatattcaat
    17941 tggctagaaa taggacaaat tctgtaattt atggtggtag taatacagtt atttcaaggg
    18001 aagcacttga agaagttgat ggattttata cttattctat tacagaagat tttgcaacag
    18061 gtatcttaat tcaaagtaag gggtatagat gttatgctat tccagaagta catgcatcag
    18121 gtttatctcc gacagattta aaaagtctta ttaagcaacg tgaaagatgg gcacgtggat
    18181 gtatacaaac tggtagacgt ctaaatattt tgtttaggag aggtttaggt ttttggcaga
    18241 aaataagtta tatttcttca attacttatt ggtatgcaag tataaaacgc tttgtatata
    18301 taatggcacc tattttattt tctgtgttta atgtgattgt agtaaaatgt acgttacttc
    18361 aagtattggt attttggttg ccaatgtata ttttaagtag tttatctctt aaaatatttt
    18421 ctcaaaatat aagaaatact agatggacca atatatatga aactataatg tttcagtctt
    18481 taatgccagc agtaatactt gaaacgtttg caatatctaa aaataaattt tcagtaacaa
    18541 ataagagtaa attagaagaa aatagaatgt ataagttttt acagggaata ccttatttta
    18601 tttacatggt cttatctatc ataggaattt taaaaatgtt tgtagcaata tttaaaatga
    18661 gttcaatgac atattcagtt gtattatttt ggttgattgg aaaccttttt aaccttgtta
    18721 tggctacatt atttatatca gggagacagc agttaagaaa gtctgagcgt tatattgctg
    18781 aaattgattt taagttaaag caaaattctt atgttctttc ttcaaaaaca attgatatat
    18841 cagaaaatgg gtttgcattt ttgctagaaa atccagaata tatttcccca gaagaagagt
    18901 ttgaggtaga gtttagagaa aaatctggaa atgaaatgta tatagctaat atgaaagcaa
    18961 agattgttaa tgttgtggaa gtaaattcaa agtggaaata tgcagcttat attacacata
    19021 ttgaggatag tgaaatagat aattggatgt gtattgttca cgatagaata cctactttac
    19081 caatgacaat atctaatcaa ctaggtttct ttgatgattt gcaaattaat gtaaaaaaac
    19141 gtattgaaaa aactagaaca ttatcacgaa gaagtccaag aatcaatatg aattttcaga
    19201 tggatataaa aaatacaggt aaattaagaa tagtgaattt taattatcaa tatgtattat
    19261 taaattttga gaataaaagt atttatccta aagaaatagc attggaaatt aatgaaaata
    19321 ttgtgttaga atgtgatttg tgtgagggta aaattgatga aagaggtatc ttatataaag
    19381 taaataacat tgacagcata atgcaaaatt tctttttaag agatgaaatg atggattgga
    19441 tattacaaaa taagacaatt cttgtttcaa agcctagtga aaagaaggaa aaaagtatag
    19501 atgagtttga acccatggaa tatatttagg agagatgaaa tgaagacttt aaaagtgttt
    19561 ttttgttcta caaaatatct tattttggtg tttatttttt ttataaatac tacttttata
    19621 agttcagcaa caagtgataa aattttagat ttatccttta aaaagataga gactgactta
    19681 agttctaaga ttacatatga ggatacaggt gtaagaatag aaacggatag ttctaaaagc
    19741 gataaggaac gttatttata tatttaccaa aatataaaag aaaattggag catgtataat
    19801 aatttttata ttgaaattca aaataaaaat aaaagttctc agagaataaa tttaagcata
    19861 caaagtaaaa atatgcttga atttagatta aaagaaggta gtgaagtatt tttagaaggt
    19921 aaaaatataa tatattcaga taaaataaaa gaaggatgca tagaagtact aggtgaattt
    19981 gaaggcaaaa tttatgtgaa ctttaatagc cttataaatg aggaaagtaa tgttgtgctg
    20041 gactcaaata tgctttcaaa cattgttagt tggggaatta cgtttattcc atctgatgaa
    20101 gaacataata ttgttattat aaaaaagata tctttattgt ctgaagagaa attaagattt
    20161 ttaaataata ttaaaataat tggcgatgaa gaagtacaaa taccagtatt gggtcaaagt
    20221 ataagtcagt atgaagtatt aggtcttaaa agtgattcta aaattaagta ctctcttatg
    20281 ggaaaacaag ataatgtgag catatctcaa aaaggtaaat taacattaag taataaagcc
    20341 aaacctggac aaattatttt acaagttaat gttgatgata agtttaaaat tggaaagaaa
    20401 ataacattaa ctgagtcttg gagtgttaat aaaaaagata aggatggtgt tccatataca
    20461 ttagtatatc ctgagcaatc accaacagta caagatatga aaaaaataaa ctttatgaat
    20521 aacatcataa cgtttgttcg aattttattt gtatctctag taataatatg ctttggaata
    20581 tatttatatt ggaaaaaatg ttcaaaaact aagtaaagag aggaggttag attgtgttat
    20641 tttcaagttt aattttttta ttttattttt tacctataac tttggtttta tattatgtat
    20701 ttaggtttaa ccgaacgata cagaacatga tattgcttgc tgttagttta tttttttatg
    20761 cttggggaga gccaaaattt gttgttatca tgatagtttc cataataatg aactatatat
    20821 ttggtttact ggtagataga tatagggaaa gtaaaataaa agtaaaagtt tttttagttt
    20881 tgatgtgtgc atacaatata ggtgttttat ttatatttaa atatctagct tttgcgctaa
    20941 gaaatattag tgaacttatt agtactgaat tgactatacc tagtattgtt ttacccatag
    21001 gaatttcatt ttttacattt cagggaatgt cttatgttat agatgtatat cgtagacatg
    21061 gagaagtcca aaagaaccct ttttatgtgg gattgtatat agcatttttc ccacaattaa
    21121 tagcaggtcc tatagttaga tatgagagtg tggcagaaca aattctcaat agaaaagaaa
    21181 cttggaataa attttctatt gggacttgta ggtttataac aggtctaggt aaaaaagtat
    21241 taatttcaaa taacatggct atagtagcag actatattta cactatgaat agtcaaggag
    21301 aaatagctgc atcacttgca tggcttggtt caatagccta tactctgcaa atattctttg
    21361 atttttctgc ttattcagat atggctatag gcttaggtct tatgtttggt tttaaatttg
    21421 aagagaactt taattatcca tatatatcaa aatcaattag tgagttttgg agaagatggc
    21481 acatttcttt gggaatgtgg tttaagagct atatatactt tccgcttgga ggttctagag
    21541 tagcaaataa agatattatg attagaaata tgtttatagt gtggttattc actggaatat
    21601 ggcatggagc agaatggaca tttgtaattt gggggatttt aaactttgtt ttcctaatta
    21661 tagagcgatt tataatgttt gaaaagattg aaaatcacaa ttttataaaa catatttatg
    21721 ctttattagt agttaatttt ggatgggtat tgtttaggtc acctaattta aaagaagcat
    21781 ataattattt taaagctatg tttggaggca atggagctat atggagtgat tatacatata
    21841 tgtttttaaa agaatactgg gttttcttta tatttgcatt tatttttagt gtacctattg
    21901 caaagaaaat caataagttt gttgttgaac aagctaaata tagtatgata tttaacgtat
    21961 tttatccaat ctcagctatt ttattgtttt ttatttctgt gacatattta gtaactggaa
    22021 gctataatcc atttatttat tttaattttt agttgggagg aaaagtatga atagattttt
    22081 taaagtaaga ttaataactg caattatgtt cttaattatt ttattcgctt tctctatctt
    22141 taatgtttgt tattctggaa aagatacgat acaggaaata aaaaattcaa tagctgaaaa
    22201 aagtagttta aaagatacaa tatcatctat tgataataca gtgaatgaca aagtaatata
    22261 caaaaatacg ttcattgaaa cttatggcta tttacaaaaa ttaatgggta aaaatgagtt
    22321 ttcaaaattt agtgttgtaa aagatacttc tggaaagatg cattatacat attttgctaa
    22381 agctccaaat ccagttggtg tgatagcaga tagggtaaga aaattttctg atgaaatgga
    22441 aaagcaaaac actaaattgg tttacctaat gactccagat aagtatataa aaggagtgac
    22501 aaaatttcca aaaggaatac cttattctta tagtaatgag acagcagata attttttaaa
    22561 tcaactaaaa aagtataatg tggactatat tgattttaga gaaaatattt taaagagtgg
    22621 tattccaaaa gatgatttat tttttaaaac agaccatcac tggaaagtag aaacttcttt
    22681 ttgggcattt ggagaattag ttgagcaact taataaaaaa tacaatatga atttagatga
    22741 aaatcattat tatagagata aggacaatta taattcaatt gtatatccta agtcatttct
    22801 tggttctatg ggaagaaaga ctggtatctt atatggtggt atagatgatt ttacattaat
    22861 ttatcctaaa tttaagacta attatactta ttatacagat tctaaatctc aaaaatttga
    22921 attaaaagga aggtttgaag agagtttaat attatcatat ccttttaatg cagaccttga
    22981 tttaatggat ggtcaatcag ataagtattt tacatattta ttggggaaca gaccattagt
    23041 taaaataaaa aatatagaaa atccagatgg attaaaagtg ctgtttgtta aagattctct
    23101 aattgtacca acagcagcat ttttttcctc tgtttgttca tctatagata tgatagaccc
    23161 tagatattat gatggagata tattagaata tgcaaaatcc catgactatg actttgtttt
    23221 tatatctgta tatccccaaa atcttacaaa agaatttttt ccgttttgta agtaaatttc
    23281 taaagaaatt ttcttataaa ataaaaaata aatggctaac tggttaaaag ttagccattt
    23341 attttaagtg ttactctaag aaatattttt tttactaaat atttctttat ctatttttcc
    23401 aagtgatttt cctacagtaa ttgatgatac catagcacca ttaacattta tcatggtacg
    23461 tcccatatct agtatagggt ctattgcaag tataccacca actaatggaa aataagaacc
    23521 aagaccaact ccagagataa caactgaaac ggccattgta gcagttcctg gaagcccagc
    23581 tattccaaac gaactaattg ttataacaat taacaacata acataaaagc tcatatccat
    23641 gtttactcca gccatattgg caagtgttac agccattaaa gcaggatata tacctgcaca
    23701 accattcata cccatatttg cacctaaact tgctgtaaag cttgatacac cctcatcaac
    23761 tccgtgattt tgtactagac tttctatcgt tacaggcaat gttcctaaac tagaacgtga
    23821 agtaaatgcc agaagtaaag cttcagttga attttttata taagtaatgg gatttagacc
    23881 atttaaagtt ataaaaatta aatgtataat aaataatatt gcaactgata tataaagagc
    23941 taatataaac tgtaaaacag atactattga actaatacct tgtgaagtta ttgtatttgc
    24001 aagcagagca accacagcat aaggcataaa tttaataaca gtcatagaca cacttacaat
    24061 tattttgtaa gatgcttcta taaaatctat aaatggtttt attgtttcaa aatgtttttt
    24121 acttaatctc tttattgagg tagctataaa tgtagcaaat attattgtag ctacaatatt
    24181 tccatttgcc atagccatta caggattaga tggtagtaat cctcttaaag tatccacaag
    24241 agagtttatt tctttaagtt cttgattttc aactaaagta ggcatattag ctcctacacc
    24301 taacttcatt atatttgcaa cgataattcc aacaattgca gctatagcag tagtagcaag
    24361 aagcatacct aaagatttaa atgtcagagt tccaagattg ttatctttca tatttataat
    24421 tacttttata atggataaga aaactaaagg tactactaac atttttaata agtccataaa
    24481 tccagaacca aataggctat accacttact tacttcttgt agccaagtga cttgagttgt
    24541 atcattagga aaaccggcaa aagcttgtat tatgattccc aatatgagtc ctagtactgt
    24601 agcatatatt gttctcaatg aaaattttat ttttttcttt tcaaattgtc taacaataaa
    24661 gaacgtacct agaagtaata ttatgaaaaa aattgtttta aaatctgtaa tcataagaaa
    24721 ttgtgataaa aatgaacttt ccaatatttt tctcctttta tttaaaaata aatttttcta
    24781 agtttaaatg atatcaatat attaaaaaaa tgtcaatatt atatcacaat ttacttaaat
    24841 agagtaaaac aagttgattt tgaaaaatgt gattaagtat agaaaaatag ggtataataa
    24901 atgtttaaag aatttgaatt atggtaagaa aataaaaaat agcttttata agatttgatt
    24961 tcaaattaaa atatgttaaa taaataacta aaaattgtta taaaaaatgt tttcatagaa
    25021 tatatatcaa atgaaaagct taaaataagt gaagaaatta actagacatc aaaggagaga
    25081 taaaatgaga gtaataataa ttggtggtgt tgcagctggt atgagtgctg cagcaaaatt
    25141 aaaaagaata aaacctgagt atgaagttgt tgtttatgaa aaaactgaaa tagtatcttt
    25201 tggtgcttgt ggattaccat actttgttgg tggatttttt aatgatgctg atgaattatt
    25261 agcaagaaca ccagagaaac ttagagaagc tggaattgat ttaaatatat ttagagaagt
    25321 cgttgaggtt gatagtgaat ctaagaaaat aaaagtaaaa aacataaaaa cagatgaaat
    25381 atatgaagat tactatgata aattgatgat agcaactgga gcaagaagta ttatgcctcc
    25441 tataaaaaac ataaaattga agaatgtatc tacattaaaa agtttatatg atggagaata
    25501 tctaaaaaaa cttttaagta atgaagataa taaaagagtg acaataatag gagctggttt
    25561 tatagggtta gaagcagttg aggcatgtaa gaaattaggt aaagatgtag atgtaattca
    25621 gttagaagat agaatattgc cacaagtatt tgataaggag atgacggatg tattagaaga
    25681 agaaataaat agacataatg ttaatttgca cttagatgaa atggttgtag aactttgtgg
    25741 agaagataaa gtagaaaaag ttataacaaa caaaggggaa atagatacag atgtagtaat
    25801 aattgctact ggcgtgagac ctaataccga gtttttgtca aatactaata taaaaatgct
    25861 aaaaaatgga gctatagtag ttgatgagta tggaagaact tctgtagaag atatatactc
    25921 tgctggagat tgtgcaacta taaagaatat agtaagtaat gaaaatgtat atgttccttt
    25981 agctactgga gctaataaat tgggtaggat agtaggagaa aacctagcag gaagagaagt
    26041 atcttatcaa ggttcactat cttcaagttg tataaaaatt atggatatgg aggcagcatc
    26101 aactggaatt acagaaaggc aagctaaaga tttaggcata aatgtcaaat ctaaatttat
    26161 ttctgattat aatcaaactc attattatcc aggaagaaat aaaatatatg taaagcttat
    26221 ttatgatgca gatacaaaag ttattttagg cggacaagtg gctgggttta aggatgctgt
    26281 ccaaagagca aatgtattgg cagctgctat ctttggaaaa atgaccacaa gtcaacttgg
    26341 aatgttagat ttatgttatg ctccaccatt tgcaagaaca tgggatgttt taaatgtagc
    26401 tggaaatgta tctaaataat atttagatta tagctaatat agttgacaag caattaaggg
    26461 aatgaattta cctaaactat ttttataatg gtaaaattca ttcctaaatt tttattccaa
    26521 atttttagaa ataaggcttc tattaaatta ttcatcaaaa tttaaacgca agcttaatct
    26581 atcttctcca agaatagtat tttcaaaaac attagtagca ttttctaaat acatactagg
    26641 tatgtctaaa gaaggactaa aatgagtcag taaaagttgt ttgacatttc caagttttgc
    26701 aagattagca gcttctctaa aagtcatatg tttgtttcta acagcttttg atatatctaa
    26761 atcatcacca tacatggctt cacatataaa taaatcacta tcttttatga aatctggtat
    26821 tgattctata ggtcttgtat cggttgtaag acttatctta actccctttc tcaattctcc
    26881 taagaccata tctggagtgt attgttttga atctaaaact atattttgac cttcttggag
    26941 tttgttccat aatattttag gaacttcgtt tttagttgcc ttatctatat caaatttagg
    27001 gtttcttcta aaattaaaac tgtatcctaa acactctata gagtgttcta aactgattgt
    27061 tgaaatttct aaatttctta aaactttatt atctaaagaa aaattgcctt gagggttttc
    27121 tataattttt aaagtgtatg gtacatattc tactaaattt ctcatagaac gtatacaatc
    27181 gactattcca actggaccaa ctatagttaa atcacttgta cgaccactat ttccaattgt
    27241 tgataaaagt cctaaaagtc caaatatatg gtcaccatgt aaatgggtta tacaaatcaa
    27301 gtctatatct ttaaagccac atttttttag cttcatagat acctgagtac cttcaccaca
    27361 gtcaattagt atttttctac ctttatagtt tataaataca gaggatagaa atctgtttgg
    27421 cataggtaca tttccaccac atccaagtaa agttaaatct atcatttttt cccctttcta
    27481 aaattaacac aaatctgttg tttgttttta ttatgatatc ctattataat tattttaaca
    27541 ttaaatataa caataaatgt gtatttgagt caagaaatat tgtcaaataa agtttttata
    27601 tagaaatgta taaaaatttg gaaaacaaaa agtgaaaatt atatttagga gcatttaaag
    27661 gaggaaaatt taatgaaagt attaaagaaa attactattt tgtcattgat tacattaacc
    27721 ttagttggtt gtagtaacgg agaagataaa aaagtggttg aaggttctaa gaaaattcaa
    27781 aattctaaaa aagatgaaca gcctataata gtacataaag atgcaaatat attgaaagat
    27841 aggtatgagc caccaaaagg atttaaaaga gttgatgtag aaaaaggtag ttttgaatca
    27901 tttcttagga atactaagct aaaaaattat ggagagaaag taaagtatta tgatggaaga
    27961 actaaggaat caaaaggtat ttatgatagt gtatttgatg ttgatattgg caatagagat
    28021 ttgcatcaat gtgcagatgc aattatgctt atgagagcag aatacttgta tcagaataaa
    28081 ctatatgatg aaataagttt tgattttgtt gatggtttta aggcgaagta ttcaaaatgg
    28141 gcacaaggat atagaatttc agtaaaagat agtggttcaa gctggtataa agcaactgaa
    28201 tattcaacat cttatgagag ttttagaaag tttatggata tagtgtttgc atattcagga
    28261 acgttatctc ttgaaaaaga attggagcct gtaaaagttg aggatatgaa aataggagat
    28321 gtgtttatag ttggtggaag tccaggacat gcaagtataa ttatggatat ggcagaaaat
    28381 gaaaagactg gtgaaaaggt atttatgata gcccaaagct atatgcctgc acaacaaacg
    28441 caacttctta ttaatcgtaa aaatccaaat ttgagtcctt ggtatagttt agattttgaa
    28501 ggtgatttga caatacctca atggactttt aaaagagacc agttgaaaag attttaaaat
    28561 aataatttta tataataaaa gaaaaatata gatgtgaaaa aatgtaatta gattaaaact
    28621 aattggatat ggacaaaatt aaattgatag ataaatatta aaaattacac taattaatct
    28681 tactatagtt taatatttat ctaaaaagtt ttatgtaaaa tataaatttt atattaaagc
    28741 taaaactaat ttgaggaggc taatttatat tgaagattag aaggtattta attttggtaa
    28801 ttataattct aattacaaca agtcagagtt ttatttctta tgggctagag cgatttgaag
    28861 aaataaccat ttttcacact aatgatatac atggaagata tgctgaaggt gatgaccata
    28921 tacaaatagg aaatctagcc acattgaaaa aagaaactcc aaactcaatt ttagtagatg
    28981 caggagattg tttgcatgga cttccaatag taaatatgga tagagggaaa agtgcaattg
    29041 aattaattaa atgtgctgga tatgactata ttactcctgg aaatcatgac tttaattatg
    29101 gaaaagatag actgtttgag ttatctaaaa gtgctaattt aggagagaat actttaaaat
    29161 ttttatcatc aaatattttt gaaaatggaa aaaaagtttt tgatagtaat gatataaaag
    29221 aaatagatgg aataaaaata ggattttttg gactctcaac ccaggaaaca aagagcaaaa
    29281 ctggatttaa aaatgtggaa ggattagagt ttaaaaatcc tatagaaagt gcaacagaac
    29341 aagtaaatga attaaaagga aaaggtgcca atgtaatagt tgcaatatgt catattggta
    29401 caaatagttt aagtaagcca aatagtatag atatagcaaa tgaggttcaa ggaattgatt
    29461 tgattattga tggacacagc cacactaagt ttgaaaatgg taaacaagtg ggtgatacat
    29521 tgattgtaag tgcaggacaa tatttagaaa atattggcga gataaaacta actttggata
    29581 ctaatgatgt agaagatata aaaatagaaa ataaatcagc tagattaatt ggaaaagaag
    29641 aagcattaaa gtataagctt gactccgctg taagtaaaaa ggttgatgaa ataaaagaac
    29701 aacaagaaaa aatattagat aaagttatag gggtaactac aaatactcta gatggttcat
    29761 atgaaaatgt aaggacaaaa gaaactaatt tgggtaattt aataagtgac attttactag
    29821 ataaaactaa ggctgatatt tctttattta atggagggaa tattcgagat acgatagaaa
    29881 aaggcaatat aacaagaaga gatattgtag atgtatttcc ttttaacaat actatagtta
    29941 ccaaagaact tacaggagca cagattaaag atgtattgga gcatggagta aaactttacc
    30001 cagaaaaaag ttcagccttt ttgcaagtgg gaggaatttc atattacttt aatcctaaac
    30061 aaaaagaagg cgaaaaaata acaaatattc aaaaagaagg aaagccatta gatttaaata
    30121 aaaagtatgt agttgcaacg aatgattata tagcctcagg tggagatgaa tttccttgtt
    30181 tttcaaaaga gccaatacta aaggaatttg gaaatttaga gagtgcagtc attgaatata
    30241 tagagtacaa aaaagaaata tcaaaaaatg tagatggaag aattggtatt aaatctaaag
    30301 atgagttaat aagcaagaat catgagagtg atttgatatt aagtgaaaat gaaaaaaata
    30361 tcaatgataa tgaagagaat ctatctaata atataaaaag ctctgatagt gataaaaata
    30421 aaagcttaga taaaacaagt tctagagaaa gttctaatct aggaatatct aaaagagaag
    30481 ctaaagaaaa atctcctaaa acaggagatt taggtttttc aaatagtata attatattta
    30541 tagtttcaag tacattgata tgtttattga attttaatca aaaagagtta aaggataaaa
    30601 agtcaaaata aaaattaatg agcaaaaact agtagatagt aatttaattt tagataatag
    30661 taaacagcaa aggaaattag ttatataaat actaatttcc tttgttttat gttttaacat
    30721 gattactttt aaataaaatt gtattttatg aaaaataatt acactaaatg gcatagtatt
    30781 atggggattt ttagttttta ttcaagtaaa tgaaatcata ttaacaactt ctgatatatt
    30841 tacaaattga taaaaaaatg attttattag tatgatataa tttagctagt atatatgata
    30901 atggaggata tagggatgaa taatatatta cttttggaag atgacaaatc tttaaataga
    30961 ggaattagtt ttaaattaaa aaaagaagga tacaatgttt ttagtgcatt tggtatagaa
    31021 gaagcaaaaa atattttcgc aaaagaagag atatgcttaa ttatttccga tataggtcta
    31081 cctgatggta gtggttttga cttttgtgag gagattagaa agaaaagtga tgtttacata
    31141 ataatgttga cagctcttga tgaagaggtg gatattgtca caggatatga tttaggtgca
    31201 gatgattata tcacaaagcc ttttagcttg atggtattaa tatcaaaagt gaatgcgctt
    31261 atgaaaagag taaatacagt aaaaaattat actctattag tttgtgatga tttattcttt
    31321 tattatatag aaaataaatt aattataaga acagatgata aggaagaaga gataatttta
    31381 agtaagacag agacaaaact attaaaatat ttgatggaaa attctatgca gacattgact
    31441 aaagagcaac tgttagaatc tttatgggat agtagtggta attttgtaga tgataatacg
    31501 attgcagtaa atataagaag acttagacaa aaggttgaaa aaaatccatc tgaacctaaa
    31561 tatataaaaa cagtaagagg ggttggatat atgtggggag aaaggagtat aaaaaaatgc
    31621 taaaaaatat gaacctcaaa gatggtttaa gtgtgaaaga tgattatata atgagaagag
    31681 ttattgtata ttctgtttta tctgtactaa tagtaaccct tgtatttata ctttttgaat
    31741 atagcagatt acataaattg tatgaacatc aaataaaaaa tactcaaaat attgctggaa
    31801 tattgattga aaaatatcct aatgatgaaa tagatatagt aaattctatt tataattctg
    31861 attatagtaa ggtgtctact ggagaaaagg catttaagaa atttggctat ggattggaaa
    31921 ataaaatgtc aaatgataaa aattttagct tatacttaaa atcttttttt aaggaatcat
    31981 tttttatttt tttaatgatg tttatagtag ttataggtat aatatattat tttataagat
    32041 acataaataa aagattgagt aaaatttatt ttatagtaga taagatgtca caagaaaact
    32101 acttgaagga cactactgag tcagaagaaa ctggttatct agtagaatat aatgaatact
    32161 ttaaagaagg catatttagt aagataaata actgtctata tgagcttaat agaagtttaa
    32221 agataaagtt tattaaatta gaaaaagaaa aagaaagtgt aaaatcatta gtaacagata
    32281 tttcccatca attaaaaact cctttagcat ccttaaaatt atacaataca ttacttatag
    32341 aagaagaatt agaagaagat gaagccaacg agttcttatt gaccaataag gcttctataa
    32401 ataaattaga aaatttaata aattctttag tgaacatatc aaggttagaa attagcatga
    32461 taaacataaa aaaagaagat aatgatataa aaaatactat tctaaatgca gtaaaaagtg
    32521 ttacccctaa ggctaagagc aaaagtataa cattaaattt aaatgaattt aacagtagaa
    32581 ttataccaca tgataaaaag tggactgagg agagtatttt taatgtatta gacaatgctg
    32641 taaagtatac acaggtaaat ggagaaataa atatatctgt agaagaaact gctaatttct
    32701 ttaaaattat aatagaggat aatggcatag ggattgaaaa aaatgagttt aataatatat
    32761 ttaaacgttt ttatagaggt tctgctgatg aaattgaatc tgtagaaggt agtggggtag
    32821 gattgtattt aagtagaaag attttagaag aacaaggtgg aaatattata gtttcttcaa
    32881 aagtaggaat agggagcaag ttttctttgt ttcttacaac tatgtaatat taaatgaaag
    32941 gaaaatgtaa gcctctacat ttattatata agtataaaca gctccagaaa taaaaaatta
    33001 ataaaatatg gagtaatata acttagaatg agaggataat tttatgaaaa tactatatac
    33061 tgaaaattta tcaaagcatt atggaaaagg agaaagctta gtaagagctt tggacaatgt
    33121 tgatttagaa ataaatgaag gtgaatttgt tgcaataata ggaaaatcag gttctggaaa
    33181 aagtacactt ttgcatatga ttggaggtct tgatatacct acatctggga aggtgtatat
    33241 agataataaa aatatattta cattaaaaga agaagaactt gctgtattta gaagaagaaa
    33301 aatagggttt atatttcaaa gctataattt aattccatct cttaatgtat gggaaaatgt
    33361 aatgttgcca atcggtttag atggtagaga ggtagatgaa ccttttataa aagagctttt
    33421 aaaatcatta ggacttgaaa ataaacacga cgttttacca aatacattat caggtggtca
    33481 acaacaaagg gtggcaatag caagagcatt agctacaaga ccagctataa tacttgctga
    33541 tgaaccaact ggaaacctag attctaaaac atctgatgag gttatgagta tattaaaatc
    33601 tatgtctaag aagtacagtc aaactctagt tatgataact catgatgaca gtatagccca
    33661 aatggctgat agagttatat tcatagaaga tggcagggtg ttaaaggttg gtgataaaaa
    33721 tgattaatac tactaaaata gcaaaaagta atttaaagca aaataaatca aaaagtattc
    33781 taataataat tactataata ctttcaacct taatgctttc ttctattgga atttatattg
    33841 ttgatgcagg agcatatcaa aaagaaaata ctataaagta ttcaggtaat tatcaaggta
    33901 tattagcaaa tgtagatgaa aaacaggctg atatattgag taatcatgca gatgttgaat
    33961 taactggtga aatgaatgga gtaggtgttg aaaaactgga agatgattca aatatttctc
    34021 tagcatacat gaatgaagat gctttgaaat taaatagttt tgaatttatc aaaggaaaat
    34081 tgccatcaaa agagaatgaa attgtgttag atagtggagc tttaaaggca cttggttata
    34141 aaaataaaga tttaggtgaa aagataaaaa tatcttatga tgactataaa agtgataaaa
    34201 aaattgaaaa agagttcatt ataagtggaa tcttaaaaac tagcgaaata tctgaagcag
    34261 gtaaatatta ttatgctatt atatcagaat catatatgag aaatactaga gatatgtcac
    34321 aagaagattt taatatttat gtgaaattta atgataaaag taatttatca atagagcaag
    34381 caaaagaaaa attagataaa attgctaatg atataggact ggatactatt aatactgctg
    34441 taaatgaaaa ttatataaat gctttaaaac cagatatgga aacgattatg ggtggagttt
    34501 ttgtagggtt agtaattgta ttatctagta ttttagtaat atacaatata ttttatattt
    34561 caatagttac aaaggttcaa gaatttggaa aacttagagc aattggtgca actaagaaac
    34621 aaataaaaaa tatagtgttt aaagaagggt tcatattagc cggaatagca ataccaatag
    34681 gaataatttt aggctatgta cttgcaaata ttataataaa atcatttatg gatatagatg
    34741 taaaatcatc acgattacca gttatattat cggttgtggt aataagcttt atatctgttg
    34801 ttttatcact tttaaaacca atgaaagtag catcaaaagt atccattgtt gacgcagtaa
    34861 gatatacagg aaataaaata agtaataaga gtaagagaaa aggatataaa aatataaatc
    34921 taaagagatt atcacatgcc aatttagaaa gaaataaaaa acgtacttac atgacattgg
    34981 catcacttat acttagtgga actatattta taacagtatc aactgcttta gaaagttttg
    35041 atgctgagaa aatggcaaga gaacactttc cttatgatat agaagttaga ctatctggtt
    35101 atgaaatgaa cagtgataaa aatcctaaag ataatttgaa tattttacaa atggataatc
    35161 ctctaagtaa agattttttt aatcaaataa agaacatgga aggaattaaa agaatagaat
    35221 ctgcaagaag tgtaaaaatt ggtatggagg actatgatgt tgaatttaag tatgaccttt
    35281 tacaaagtat aaatgaaaat gatgtaaaat cattaaacaa gaatataata gatggaaaaa
    35341 taaatcttga aagacttcaa actggagatg aaatagtaat tactcatgtt gatacagcaa
    35401 aagaaatggg agtaaaagca ggagataaaa taagattaac tttatatgat ggagataaaa
    35461 agataaaaaa agaatttaaa gttcaagcaa tagctatggg ggttccaagt tttggtatag
    35521 ggaaggattt tattgataga actttgaaat acgattcaac tagtagcttg ggaatatata
    35581 cagaagaagg aaagtatcaa gaaatcaaag atagtatcaa gaagattgct aaaaataatg
    35641 gatttttaga gactgatttt atagatagta gaattgagtc aaataaggct actatttcat
    35701 ttataaagat aatgggatac acgctaacag gcataatagg tgtgattgga tttatgaatc
    35761 ttgtcaacac aatgataact agcatagtta ctagaaaaaa agaacttggt atgcttcaag
    35821 ctattggtct tactaataaa caattagtga aaatgcttaa ttcagaggca atgtattaca
    35881 cttctggaat gatgatagga agtattctat ttggaggtat attagggtat attgctgtta
    35941 tatttctaaa aaagactgga ttatcttatg caacatattc tttgccaata gtaccaatct
    36001 tgttgatgat tgtgtgtatt cttatagctc aatttataac aacttatttg ataggacgaa
    36061 gttttaataa agaatctctt attgatagag taagatacag tgaataaaat acaaatattt
    36121 atggttagtt taactagcaa aaaacatata aaaaaatata gttaaagaga ttcataatta
    36181 aataggttaa agcatagtac agagcaaaca atttaagcag atgtttgctc tgtattttat
    36241 tatgtaatca ataatatatc taatatactg atattattca tgttagaaaa tgtaccggta
    36301 caattctaaa aaagtataaa attgtaccat ataaaatgaa aattttgtat aataatcgta
    36361 tagaaaataa ataggagctg atatatatga ataagaagat taattatgca ccaggaccaa
    36421 ctgaaactag ggaaaatgta agattagcaa gagcagaaaa aacaacaaat ccagatatag
    36481 atattgattt tgttgaattt tataaaaaga catgcgagaa gtttggtaat ataattggaa
    36541 caaaaaacga tgtttacata ttgagtggtg aaggaatatt aggtcttgaa gcagcatgtg
    36601 catcacttac agaaaaagga gatagagtac ttgtaattga caatggtata tatggagaag
    36661 gttttaaaga ttttgtaaca atgtatggtg gggagtatgt gcttttttca agtgaatata
    36721 ctaaaagtat agatattgat gaattgaaag catttttaga tgaggatagt aattttaaat
    36781 atgcaacagt agtgcattgt gatactccaa ctggcgtatt aaatgatgta agcaaaatat
    36841 gtccattact taaagaatat ggaatattaa ctgtagttga ttctgtagca ggtatggtag
    36901 gagaaagatt aagtgtagat gaatctaaaa tagatataat attaggtggt tcacaaaaag
    36961 ctatttctgc accagcagga cttactatag tcggaataag tcaagatgca aaaaattgta
    37021 taaaaaatag aaaaactgat gtaataggtt tttattgtaa tttaaatata tgggaaggct
    37081 attatgaaaa aaaatacttc ccttatacaa tgccaataag tgacataatg gggcttgata
    37141 aagcacttga taatatatta gaagaaggaa tagaaaatgt attaaataga catgaaaaga
    37201 tagcttatag tgttagaaaa tctattgagg aatatggtct agaactattt ttagaagaag
    37261 gatattctaa tacagtcaca gctataaaag ttcctgaaag tataggagct ttagaactta
    37321 ctgattatat gcttaaaaat tataatacac ttgttgcaac atcactaaat caatatatgg
    37381 atactatttt aagaataggt catatgggag agaatgcaaa tctaaataaa atagaacatg
    37441 tactaaatgt tttggataaa agtttaagtg cactaggatt caaaggaaat ggaaatttgt
    37501 taaatttatt taataagtat tatttttaat agataggggg atatagtatg gaagttacag
    37561 ctaagcaaaa atttacaacg aaagacatgg tggaaacctc actattaata gcattagtat
    37621 ttattgcaac taaatttata aacataagat taccaatatc tataaatggt gggcttgtgc
    37681 atttaggaac tgcaatgttg ttcatatcag caatagtatt tggaagtaaa aaaggtgctt
    37741 tatcaggagc tattggtatg tcactttttg atttgatatc tgggtggact ttatgggcac
    37801 cgtttacctt tatagttaga ggagttatgg gttatttact tggaaagatt gcatgggcaa
    37861 atggtaataa tgggaataat tttttaatca atgtaatagg aatatgtgtt tcatcaattt
    37921 ggatgctatt tggatattat gtaacagaag ttatacttta tggtaatttt atagtacctc
    37981 ttacttcaat accaggtaat ttaatgcaag tattgatagg ccttataata gcgcttccta
    38041 tttctaaggt attgaaaaaa tgtataaaat aaatataaag tgaactaaac tttatgatag
    38101 cataaattat actgtataat attaggatgg agtacataat taaaaaattt tagtatagag
    38161 gttagttcac aatgttatca gttacaagat attcaatcaa aaatgtccgt ttaaagccgt
    38221 gggtaaagtt tatttggtat tttgagacaa aatcaaatat actgttaaat aataagttac
    38281 ttccgacaga cagtatagat attattctca atctttctga tgttatggaa tataaaattg
    38341 aaaatcaaga ctatactgct agtaatatgc attttaatgg aataagggat aaacatggat
    38401 ttattattca acatggaaat atacgtgtta tagggatttc tttttatcca tttggtctct
    38461 atccattttt aaagatacca atttcagagt ttaacagaca aattgttgac ctagaagcag
    38521 tatcacagct atttgcaaaa aaattggaag agtcattaaa tccaatacaa tcagtagaaa
    38581 aaattgtttt atatttagaa gaagtattat tatccatatt agaagaagat ttaatttcaa
    38641 ataaatatgt aaagcttcta aattcattta tatatgttaa tagatatagc aatataaaag
    38701 cattttgtga tgatacaaat ataaatatta aaacattgga aagaatctgt ttaaaatata
    38761 caggatatac acctaagatt ttaaagagaa tttacagatt taaaatggca agcaatcaac
    38821 ttatttataa ttataaagat gatgaacttt ttgattttat ttatgaaaat gagtactatg
    38881 accaagcaca ctttataaaa gaatttaaaa aattttcagg gacgtcacca attaaattta
    38941 gtggtgagaa taagacgatt aaagaaaata taaagtacag ctatttataa ttatactgtc
    39001 gattttttac aatacctcca atttattttt tgatatgata taggaaaata aattggaggt
    39061 tttacatatg aaaattatta cagaaatagt tgagtttaat gtggaagaaa atttatcaaa
    39121 agaagagttt atagaaattg taaatgactt agaattaaaa tttcattcta ttcaatctgg
    39181 ttttattgat actgagcttt tatatgatga aaagtctcaa aagtggatta tgatacaaca
    39241 ttgggaatct atagaagacc tcaagagtgc ttctagtaaa atgtttaaag attcttcaac
    39301 tgagaaattt agaaaagctt taaagaatca aactgttaag atgaatataa taccacaaat
    39361 taaaagttgg aaattataga acttaatgta aaagatagtt aaatagtatc aaactaaatt
    39421 agccaaataa cattttgtat ttaaattgtt atttggcttt tagtgtttat tttaatatga
    39481 gtgttatata ctagaaaaac caaccttaaa tactactatc taaaagcact attgctgtac
    39541 gagaggcaat aggcatttta gaagaaggag gagttgagct aaagtataca tcataatctc
    39601 ttaagttttc tttttttgtg tttcctccaa actctaccat atcagtattt aatatcgttt
    39661 tgtagtttgt attttgaaac tttgaaatat caactgattg tattagatta gggtggaaat
    39721 taaatataaa taaatatttg cccttactgt acaccaaaat ctttttatca ttatccatat
    39781 gtataagttc atgactattt tggataaaaa tatcactgta ttttgtaagt tcgagcatag
    39841 atttatcaaa atttaataat tgtttatact ttaagttgtt atcttctgaa aggctccatt
    39901 gtcttctggc atacttatag ctccaattgt tgccttctcg tggaaaatca atccactcag
    39961 gatgtccaaa ttcatttccc ataaaattta gatatccttc acctgctaga gaaaaagtta
    40021 ttaatttaat taatttaata agagatattg ctctattgat aacatggtta tttgagttta
    40081 tttccatatt ccagtacatt tccttgtcag ctagccaaaa aataattgtt ttatcgccaa
    40141 ctaatgcttg gtcatgggat tcacaatatc ctatattttt ttctccaggt cgtctagtag
    40201 taagttcata ccacattttt cccaaatccc agtcttcatc agataagtta gagatagttt
    40261 ttatccaaaa atcaggtact cccattgcga gtctatagtc aaatccaatt ccgccgtctt
    40321 ttatagggat acacatacca ggcattccac tcatatcttc tgcaatactt atcgaattag
    40381 gtttaatttc ttttattaat tcatttgcaa attggagata tgttatagct tcaatatctg
    40441 tattcatact aaaatatttt tcataactat caaatgaaac tccaagacca tggttatgat
    40501 ataacattga agtcacacca tcaaacctaa agccatcaaa atggtactca tttaaccaaa
    40561 attttatatt tgaaagcaaa aaatgtatta cttcaggttt accatagtta aaaagttttg
    40621 taccccaagc aggatgattt ccctttgaac ctgagtggaa aaattgatgt tcacttccat
    40681 caaactcatt tattccttct aaagtattct ttacagcatg agaatgaact aaatccaata
    40741 aaacagcaat ccccattgaa tgagcagtgt ttatgaggtt ttttaagtct tcaggagtac
    40801 caaacctaga tgagattgca taaaagtttg aaacttggta tccaaaagat gcataatatg
    40861 gatgttccat aatagccata agctgaatgg tattgtatcc agcttttttt atcttaggaa
    40921 gtattttttc agtaaattca ttataagttc caatagattc gctttcagta gacataccta
    40981 tatgacattc atagattagt ggagaagtta tgttttttaa gtcaaaatca ttgtcagtcc
    41041 agataaatgg agtttttggt tgccatattt gtccattaaa tccaccaaaa tctttttgaa
    41101 ttactctttt tatatataat ggtatacggt caaatgtttt tccatttgca gtaacttgta
    41161 cctttacctc tgatttgtga ggtaagctat tttgaccagg aataaatatt tcccaattac
    41221 cagacccaat ttttcttaga ggatgagatt ttctattcca attattaaag tcaccaatta
    41281 gtgacaaact atctgcattt ggagcccatt ccctatagaa gaatccatca tttgtctgat
    41341 gaaatccata gtaaagatga gcatttgcaa aagaactaaa atctttatag tttttcaatg
    41401 tttttgattt catatcctca taatgttcca ttcgcttttt tatatcagat tcaaatggtt
    41461 gtaggtatac atcaatatct aaaattttaa atcttttttg acttttcatt gtatttctcc
    41521 tccataataa atattacatc taaaaaataa tatttatctc atactatctt ctctgtataa
    41581 ttgatatata ttatataatc ctaataaaaa gcttaagcca ccaatgatta atttcataat
    41641 ttacctccct agtatttata cttatatatc gtattcatca aaatttaaaa gtttgtttaa
    41701 aactctttta ataatatcat aatcaaagcc tttataggaa agatgttgag aaattttttg
    41761 atatattttc tttttatctt ctttctttac tctttcatac cttttttttg ctagatacat
    41821 agcattttca aattcagtgt ccttgtctaa ttcagatact gcttcattga ttgtacttct
    41881 attgatacct ttattatata aattctgctt aattctgttt ttgccacatt tatttaggtt
    41941 aacatttgtg ttaacaattt tttgagctaa tacactgtca tttacgagat tataactttt
    42001 aagaaaatct atgaccctct caattatatt ttcatcaaat tctgatgata gtttttcttt
    42061 taattttttt tcagattggt cagcatgaga taaaatattt aatgctttat ttttagcttt
    42121 tatatacatt tcatcatcta aaataggttt taattcttct tcatttattt cattcccttt
    42181 ttttaaatta aatgtataaa caagttcttt aaatatagca ataaaaaatt tatcatctac
    42241 ataaatatta actctatcat catttctttt ttgttgttct atttttgtaa ttatacccat
    42301 aagtcctcct tttttatatg taattatatt aacaaaagca actaataaaa tttaacaaaa
    42361 aacttatatt tgtatgttat attttatcaa aattaagaca aaatagtaaa aagttatttt
    42421 tattaaaaag taaagcagaa tcatgttaaa taattatttt ttaaagtgtt ttttagcata
    42481 taaatataat tatataccca aaaaaacaaa ttataagctt taagaaataa aaaataatgg
    42541 tacaatagta ttataacatt aaaaggagac aaaatatgag aagtgaaaat ctagttaaga
    42601 tggcagagtt gaaaaatact gaaaaactag agaaatttaa tcgccataaa ggcaaaatca
    42661 aaataggtat attgggaggt acttttgacc ctatacacta tgctcattta gcaacagcag
    42721 aatttattag agacaagtat gacattgaca aaataatctt tataccatca ggaaatccac
    42781 cacataaatt atgtatcact acagataagt atgatagata taatatgaca cttctagcaa
    42841 ctgaaagcaa cgaagatttt ttagtttcaa aagtagaaat cgaaagaaag aaaaggactt
    42901 atacaataga tacattaaaa tacttaaaga aaaaatacaa aaatgcagac atttatttta
    42961 taacaggtgc agatgcaatt tgtagtgttg aagagtggaa agatgtaaag aaaaactttg
    43021 agttagctac ttttatagcc gcaactagac caggaattag tttgttaagg tcacaagaga
    43081 ctatagaaaa actaactaag aagtataatg ctgacattat aacagtttat gttccatcac
    43141 tggatatatc gtccacatat ataagggaac agctaaatga aggcaagtca atacgttact
    43201 tagtacccga aaatgtagaa aattatttat atgaaaataa actatatcaa tatggagatg
    43261 attaaatgaa cttagaaaat attaaccaaa gactaaatca aatgctacca gtggggaggc
    43321 ttagccattc aaaaaatgtt gcaaaatgtg ctgaaaaatt atgtgagatt tatggatgtg
    43381 acaaagaaaa agcttactta gcaggaatga ttcatgattg tgcaaagtat ttaagtgaca
    43441 aggaaataga ggattatgta aataagtatg aaatatattt agacccaata gaggatggaa
    43501 atcgttcctt gtctcatagt gtaattgggg cgtacatatg cgagtatgaa tttgaagtag
    43561 aagatgaaga tattataaat tcaatcaaat atcatacaac agggagagag gacatgtctc
    43621 ttttagaaaa aataatttac atagctgatt taattgaaga aggaagaaaa ttcccagttg
    43681 tagatacatt aagagaatta gcatatggag ggaagcttga tgaagcactt ttaacttctt
    43741 ttaataatac tttaatgttt gtcatcaata aaaaagaaga gatacatcca agaacagtta
    43801 tggcaagaaa ctatctaatt aaagaaaagt tattataaaa aagctatata aaggcaaaca
    43861 tattaataaa tttaaatttg actatgttac aaaatcgtat taatatgtta caataactaa
    43921 gtgtatttta attaggaaag tgaggaattt ttatatgaca gtggaacaaa tgacaaaaat
    43981 agcatatgat gccatagaag ataaactggg tcaagataca gttataataa atataggaaa
    44041 agtttctagt ttgtgtgatt attttattat tactacagca tcttctcaga gacaggtaaa
    44101 agctattgct gacaatgtgg aagatgaatt agcaaaacta ggattagagc caagaggtaa
    44161 agaaggacaa ggaactcaaa cttgggtact ccttgattat ggagacataa tggttcatgt
    44221 atttaatgag gaaaatagag ggttttataa tttagaaaaa ttatggaaag atgcacctta
    44281 tatagatatt gacacattag cataaacatg ataatatatg ttattataat tagaaaattt
    44341 caaaaattat attaaattga atagactaga gcagtaaaag attatatttt ttcagagagt
    44401 tagtggaagg tgagaactaa tattatataa ctttgaactt actagattaa aatctattct
    44461 ccatggctgg attaaaagct atctaagaga gtctatatat gtagtaggct aattagggtg
    44521 gtaccgcgaa gatttatcct cgtccctaaa cgtaagttta gtgacgagga ttttttattt
    44581 tcaaagaatt atttggaaat tataagtaaa taatactaaa taaggtagtt atacacaatt
    44641 aaaaatagga ggcaaaatat gagcgtatat aattttaaag aagtagaatc gaaatggcaa
    44701 aaaatttgga aagataataa tcagtataaa atggatactg cacaaactga aaaacctaat
    44761 tattacacat tagaaatgtt cccttatcca tctggaaaaa tacacatggg acatgttaga
    44821 aattattcta taggtgatgt tgtcgcaaga ttcaaaaaaa tggaaggata taatgtactt
    44881 catccaatgg gatgggattc ttttggatta ccagctgaaa atgcagctat aaaacatggg
    44941 atacatccgc ataaatggac aatggaaaat atagaagaaa tgaaagagca attaaattta
    45001 ttaggtatta gttatgattg ggataaagaa gttgcaactt caacgccaga atactataga
    45061 tttacacaag aaatattttt gaagttttta gagcatgggc ttgcatataa gaaaaaatca
    45121 tatgttaact ggtgtccttc ttgtgagact gttcttgcaa atgagcaagt tgttcaagga
    45181 gcatgtgaga gatgtaaagc aacagtatta aagaaagatt tagaacaatg gtattttaag
    45241 actactgaat ttgcagaaga attacttaat gacttagata cattagatgg atggccagaa
    45301 aaagttaaaa caatgcaaaa aaattggata ggtaaaagta caggagcaga tttagttttt
    45361 gatatagatg gaactgataa atctatgaca gtatttacaa ctagaccaga cacaacttat
    45421 ggagttactt atatggtact tgctccagag catgaattag ttaaagaatt ggttgcaggg
    45481 actgaatatg aagcagatgt tgaagctttt gttcaaaaaa tgcatactat gacagaaata
    45541 gaaagaacag cagctgatgt agaaaaagaa ggaatgttta taggtagata tgttataaat
    45601 cctttaaatg gcaagaaagt tcctctatgg atagctaact atgtattagt tgagtatgga
    45661 acaggtgcta taatggcagt tccagcacat gatgaaagag atagagattt tgctgaaaaa
    45721 tataatttag acataattga tgtaataaca gaagataaca aaatgataaa ttcagaagaa
    45781 tttaatggat tagatgcttc agaaggattt gaaggaataa tcgataagtt agaaaaagaa
    45841 ggtagaggaa agagaacaat taattataga cttagagact ggttagtttc aagacaaaga
    45901 tactggggtt gtcctatacc tgtggtttat tgtgatgaat gtggaatagt tccagttaaa
    45961 aaggaagatt taccagttct tttaccaaca gatgttgaat ttactggaaa aggggaatct
    46021 ccacttacga cttcaaagca atttatgtca acaacttgtc ctcactgtgg aaaaccagct
    46081 agaagggaag ttgatactat ggatacattt gttgattctt cttggtattt cttaagatat
    46141 gtggatagca ataatgaaaa tgaaccattt agtaaggaat tagttaatag atggcatcca
    46201 gtagaccaat atataggtgg agtagaacat gctataatgc atttacttta tgcaagatgg
    46261 tttgtaaagg catttaagag tatgggaatg gtagacttca atgagccatt taaaaactta
    46321 cttactcaag gaatggtact tatggatggt tctaaaatga gtaagtctaa agggaataca
    46381 gtatctccta tggatataat agatgagtat ggagcagata ctgcaagact tttcgtatta
    46441 tttgcagcac caccagaaag agatttggac tggtcagagc aaggtgttga tggatgcttt
    46501 agattcttaa atagggttta tagattggtt gacgagttag cagatgtggt taaaaaggat
    46561 gtagagtttg gagaattaaa ttcccaagat aaggatatga gatatactat acactctaca
    46621 cttaaaaaag ttactgctga tttaagtgaa aaattcggat ttaatactgc tatatcagct
    46681 cttatggaat taataaatga tatgtataaa tataaagagt tagataatat aaacgaagca
    46741 gttataaaag aaggtgtaca aactatagta actataattg caccatttgc ccctcattta
    46801 ggtgaagaac tatggactat gataggtaaa gaaggaagtg tatttgatat agactggcct
    46861 aaatatgacg aaaaagcttt agtaaaagat gaaatagaag ttgttgtaca agttaatgga
    46921 aaagttagag gaaaattaac tgttaactca aatatatcta aagatgagat ggaaaaggta
    46981 gctttagaag atgaaaaaat aaaaggatta gttgaaggta aaactatagt taaagttgta
    47041 gctgtaccta aaaaattagt aaatatagtt gttaaataac tcaaaaatat gaacaaaaat
    47101 ataaatttat aaaataaatg aggctgtctc aaaatagaga taagcctttt ttcattgttg
    47161 aaattataaa aatagatttg attttataca aaaaaatgat taaagaacta aaaagttcat
    47221 tcttatatac cctagttgtt gttttaaata ttattataat taatgttaaa tcgagttaat
    47281 gtattaagtg gtatgtaaat cattcagttt ccaaagtgtt gttcgtcaag gaactgctct
    47341 aagcttaaac gaaaaaaata taattattgt attttgaaat tttttctata taatgaaaac
    47401 aggcaaaaaa ggtgcatatc tatgctttat atgaaacacc cagacaacga aattcagatt
    47461 catataatag ctaccatgtt tctaaaaagg aacttgtaaa aaagatagcg tttcaacttc
    47521 ccagttgatt aaaattatca gcaggaaaac aagtatggtc agcagtggta ttctaatggg
    47581 tgttgtcgat atatccaagt tgggattgtc tgaggatgtt atcaaataac ttgtgatagg
    47641 tgcagtagat gtggagattc ctgatacgga tttattctca ggggatggtt gcttcgggag
    47701 atatggagcg taaccagttt attgatatgg taaccatttt ttgtctaatt tgatgaaaga
    47761 atcccaagaa taccatacag aagtaagaca ggaattaact ggtggtataa actgataaag
    47821 gcaaatttaa aaattttgat aggatgattt taagatttat cttctatttt gttttttaga
    47881 aataattgaa atgttatgag gaggtatgta caaatggaga ctgtaacaaa taatcaatca
    47941 ggtgtagaaa ttcaactcag tctgtttgaa gctatcaaag atttattgtt aattaatcct
    48001 attttcttgt tgggaattat cttgattggt attttcttag ttgtaatatt caagaaaaat
    48061 agtgatattc caaaggtcaa aacatctatt ttatcactgg caatgtatta ctacttatgt
    48121 ttaacactat cgcatattgt tggtatacct acattcggtg aatatataag actgtcacag
    48181 ttaggggaaa cgttctttaa tccaaacata aatcttattc cttttagtga tggatttagt
    48241 ttgagtttta ttttgaatat tttacttttt gtcccattag gttttttatg tccgcttata
    48301 agcaaaacct ttgaacgttt gagaaataca ttcttgattg gttttggatt atctcttttt
    48361 atcgaaacta ttcagctttt tacgttacac agagcaaccg atatagacga cttactcacg
    48421 aacgtaattg gaacaataat aggctatttc tgcttcaaac tgattgctaa attgcgaatt
    48481 gtaaaactac attcagatga tgactctatg gaacaggatt acacagtatg tattccgatt
    48541 gccattataa tagttgccct tatattagga ttttttagtt aagttccaaa tagctaattt
    48601 gggaatccag acaatataac agatactatg ttacctttta caggagcata ctatctgtta
    48661 tattgtcaac atcctatttt atggtttgtg tgtagtttcc tgtaaactga actaaataat
    48721 taaatagaag ctattagaat taataataac agatagatat taataagcat aaacaaatag
    48781 taaaatgtgt agatatagac gtttttaagt gaaactatta taaaataatt gactgataat
    48841 caactgacaa aacctatttt tttaggttta actgacattt aactgacagt cagtttttac
    48901 tatttaaata gaagttcgct tagattgtcg gaagcttctt tgtccatttc tttaagtaca
    48961 tgagaatatc tattcatagt tatttttata tctgtatgac ccaatctttc agaaacaact
    49021 ttcatattag ttccccctaa aagcagcaga gtggcacttg tatatcttaa atcatgtatt
    49081 ctaatctttt ttaaattatt ttttttgtaa aattgtgaaa cgtttttctt agtgaagaag
    49141 gtagaaacgg atttaaattg gtgtttagac aaactaagtt atttctgttt tccaggatac
    49201 cttacaattt taatttgctt tgtcttagtt tttcaatttt caatttttca attaattctt
    49261 ttggagctga taaagctctt tttgattttg gtgtttttgg ctctttaaaa gatattttgc
    49321 cattagaata gacaagaatt tgattaacat ttatcagatt gttatctaag tcaacatctg
    49381 accacctaag cccaatagct tctcacattc ttaaacctag aagtaacatt aaaagaataa
    49441 gtatttctat attattacca agtaactttt ctattagcaa taatacttct tctctattgt
    49501 atatttcatt tgtagaatat ttttctttag aaggaagttt tataaaatca catgaatttt
    49561 cttgtatttc tcttaatcta tacgcttccc ttattacaga acttagaaag ctatacctta
    49621 ctttagcact agtaaaggta gaattattaa agttataatt tacaaaagct tgtactgaac
    49681 taggatttac ttctgtgagt tttagattct taaaaaaggt tctatgtaat ttttaaccca
    49741 agattttgta tttgtaacag tatatggaga ccaattattt ttattcatgt ctatatattt
    49801 ataacatctt tctacaaatg tcataccttt tggagcaaca aacttattat tatttatagt
    49861 agactttatt tcaattaaat gtttttcagc atcctttttc tttttatagc ttccataact
    49921 tttttgtttc tttttgccag tttcttattc cacatataca tagaattttt cccatctttt
    49981 tcttataaaa gctgatttga tattcatata tgtacgctcc ctttataagt tagttttata
    50041 aaagaacagt tggactgctc taattatgtc attatctttt taattcttta cagttacatt
    50101 ataacacgta tttgtataca cttaaattaa caggaagata aatatcgtta aaggaggaaa
    50161 agaaaattta atgtagaatt atgtaagata tatagtattt catatataaa agggggagat
    50221 attgtgaaat taaagaaatt attatcacta gggttagccg tgtcaatgtt tgctgctggt
    50281 cctatatcag ctaatgcact tgatacaatt tatactattc aaggaaaaga taaatatgaa
    50341 acggctgctc ttatagctga tgaacaaaat tatactacag ctatattaat taatgcagac
    50401 agcacaatgg ctgatggatt aagtgcgagt ggacttgcag gagcaacaaa tgcaccaatt
    50461 tttttaacta agaaaaataa catacctaat gcaactctta aaagagtaga gaaagctaaa
    50521 aaggtataca taataggtgg agaaagttct attgataaag caacagaaac tcttctaaaa
    50581 gataaaggaa tagaggctaa aagacttcaa ggtagtgata gaattaagac aagctataat
    50641 gttgcaaaag aaataaactc aattaacaaa gtaaataaag ttatattgac taatgctttt
    50701 aaaggagagc ctgatgcaat gagtgctgca cctgtagcag ttagagataa agcagctata
    50761 gtattaactg atggaaaaag tgttccattt aatacaactg gtgtagaaag ttatgtaata
    50821 ggtggtactt catcaatgag tgacaaacta gttaatgata ctaattcaac tagattaggt
    50881 ggagtagata gatatgatac taataaaaaa atagtaaata agttctataa tggcgcaaaa
    50941 gaattttata tagctagtgg tactgattta gtttatgctt tagttgcttc accaatggct
    51001 aaaaataatc ctattgtact agttgatatt ggaagtaata aagatatact taaaaatgct
    51061 gcaaaagtga ctgggatagg aaatctaagt gataaaatta tgctagagtg tgaaaatacc
    51121 atcaataata ttagtcttcc gtcttcaaat aaaaatataa tatgtggatt aaagattgat
    51181 tttgattcaa agcacataat ttggcaagat aaaggaagtt atcttgaatg ttatgatgat
    51241 aaccttgaat attatactga aaaatataaa gacccaacag ttcctataaa tatagaaaat
    51301 acaagtacac aaaatttaga tgtaacaaaa ttaaaagttt atggagaagc taattcaaca
    51361 ttatatatgg ggcaatatgc taaaataaaa atggatttta atagtttcgg tgtattaaag
    51421 ccaggagaaa aatgtactat ttatttagat ataatttcat tagatccatc aagaagcatg
    51481 acattaacat tttcttatgt aggtgataaa gaaaattata aatatgaaca aggattggca
    51541 agtagaatat tattttataa cccagatgga gaagattttt ttgaatataa ataactagtg
    51601 ggtatgtaaa ttaataaggg ttttataagt ttaatttttt tattttcgtt ttatattaac
    51661 tatgtggtat gtaaatgtct cgatattgct catatgttga gttattatat ttagtttcat
    51721 atgaactata tggattttaa aattaaaaat acttaaaaac atttacttta atagtaggtg
    51781 tttttttgat taaaagaata ttattataat gtaaaaagta ataaataggt aaaatatgta
    51841 agaattatat gttataatat ttttagcaag aagatgtaat ctacaattta tagagtggag
    51901 ttcatgtatc aaaaaattat cctcccgacg ctaagaaggg aggtgaatat acatggataa
    51961 ttttttacaa ggcatactag caagtctatc tgctagctta atagtttata tagctagcaa
    52021 actatttaga aagcgtaaaa aaccactcaa agcggcaact aagagtggtt gggaatttga
    52081 tttaaaaatc agattccata aaactaagta atttctaaat tatgaactcc actctacagc
    52141 aaaatagatt gtagttcttc ttgcttttat tatatcacaa attggtacag atattcaaaa
    52201 ataatatttt tatgatataa taaagtcata gaaattttgc agtgttcgat tttttgaaga
    52261 aattaaggct taacagttga aatataaggc attgaggact tgtgataagt gttatcaatt
    52321 gcactactca tggttcactg caaatttgag agatttgtat atgtgtaggc attggaaata
    52381 ctcaatttat tttggggttt tatattaact atatggaatg taaatatttt ttttatatat
    52441 aaacttctaa acttttacaa aagttttata ttaactatat ggaatgtaaa ttaaatcgta
    52501 tttataatta tatctacact tagtaaatgt tttatattaa ctatatggaa tgtaaatcca
    52561 atagaccctc caataactcc agatattcct tctggtttta tattaactat atggaatgta
    52621 aataatgaac tatctaaacc aaggtcataa tttaattgag ttttatatta actatatgga
    52681 atgtaaattg tatccaaaaa attacaataa tcatacgaat tgctaatata agcagtcaat
    52741 atatgtgtac atttatccat tttatctcta aacatataaa ttattatccc cctaaaaaac
    52801 tttcataaaa acaaaaagaa caatatctct ttggcaactg gctaacataa ctcataagat
    52861 atttatataa gtcttagtcc ctatagcttt gcgtcactag atttctctag ttttgccgat
    52921 ttagttttat tctataacta aataatacaa tagaattagt tattattcaa caagattgtt
    52981 tgaaaattga ataaatttat aggtatgaac gtaatttcac acttaaaaac agactaaata
    53041 cgtagaatat atagaaaaat ttacctgtag tttttgtgat aaaaagctcc tattattaat
    53101 ttaagctaga taagtttgag gaagtgacat aaattgaaag aaggaatata ttatgttgaa
    53161 taaaaaatta ccagatgcag aattaaaaat tatgaaatac atatggaata ctggttataa
    53221 aactgtaata tcaaaagatg ttgcagatta aatagaaaaa acatatgctt ggaagcatac
    53281 aactacaatt acacttttag ctagattaac taaaaaaggt tttttcatat cgcagagaat
    53341 tgggaaacat gtacactata gagtattaat aaaagagaaa gaatatctca aaatagaggg
    53401 aaaaagaatt tttggaggtc tacataacaa tccattatca gaactaattt caaaattaca
    53461 tgatggagaa gaaataactg aagaaaaaat aatagaaata ggaaattgga taaaaagctg
    53521 gaaagatgaa gattaataaa taaaaggtag ctactaatat aagtgctacc ttttttcttg
    53581 tggataatat tggggataag ttgtggaaaa tttgtgtaaa aattgtggat aaaatatttt
    53641 gctgacattt aactgacata agtattagat tttgatttaa aaatgttgaa atatcaataa
    53701 ataattaaat agaagttatt agaattaaca atagattcct ttttcaaaga agtctttgat
    53761 ttcatcaagg ttatcagttt ttccaagtgc taagataagt tttattctag ctttttgacc
    53821 tggtaagtcc ccaccaaata tacaaccaag attttttaaa tctctaccag aaccaaagta
    53881 accataacta tcgaatactc tacctgaatg acatctagaa actataacta caggaagtcc
    53941 tttttctcta gcatattcaa ctccagaaag catttgagga ggaatattac ctctgcccat
    54001 tgcctcaata actatacctt tataaccact atcaacagca aatcttataa agtcagcatt
    54061 ttctcctaca tatgctttaa ataaggcaac tttagattct accttatcag tatctataat
    54121 tgttctattt acaatatctc ttgtaaggac aagttcgttg cagtcaatta ttcctaatgg
    54181 gccactagtt agagatttaa atgtatttag agaaagagta tttgttttag tagcttcaga
    54241 agctaataat acttcattgt ttaaaactac aaggactcct tttcccatag catctttgga
    54301 tactgcagta caaactgatg cagataaatt acttgaacca tcatatccaa gttcagaact
    54361 acttctcata gcacctgtga ctattacagg tttgatgttg tcaattgtaa ggtctagaaa
    54421 ataagctgtt tcctctaaac tatctgtacc atgtgttatt acaacacctg taatatcttt
    54481 tcttgctaat aaatcattta cataattttt caattccatc attctataag gtgttatgtg
    54541 aggtcctgga atttcatcaa aattaaaaac ttcaacatct gcaactttat caatgttggt
    54601 aaccattgat agtatttgtt cacctgataa agttggtata gctgctccga ctttatcatc
    54661 tactgtcata gatatagttc caccagtaaa aactatggca acttttttct taatactcat
    54721 actattctct gcctttcata aataaaattt ataattccga taggattata tcaaaaaaag
    54781 tagttaaggt ctacataaac tcttttattc atataattta gatatttcag tgtaatataa
    54841 ttatttctta attttttgcc attcaacgat ataaccattt aatatctctc ttatggcttt
    54901 tcctatctta gtataagctt catcatatag atttgctaga gatattctaa ttgaccattc
    54961 agggccatag aatccatttc cactaagtaa aactactgaa tagtcattag ctaatttata
    55021 aagaacatct actggagatg cagttttacc tataaattta aataagtctt ctccgtagtt
    55081 taatttagcc cattcttcaa tatcaaattg agtgtaatat gcagcattgt ttttgttttc
    55141 attaattgga agctctaaag cttcaaataa tagtttttga cgtctatggc agatactcat
    55201 attaagtttt ttgtaggcat ctactttgtc aatcaatgca aaagcagaga aaaacgccat
    55261 ttgtacttgc tgtggagtag aaagaccagc agtatggttg agagcaacaa gtctgctatc
    55321 agcaacaact ctttccataa aggaaagact agaagggtta agactcatat cactgtaacg
    55381 cttatctact gattttttta attctccagt taaatcattt attttcttat caaaaacatt
    55441 tttcttatga agggcaaatg taccaagtct ccatcctgta actccaaaat attttgaata
    55501 agaatatact ccaacagtgt tgtaaggtag ttttgacatt aaggatgaaa aatcttctac
    55561 aaaagttcca tatacatcat ctgatattat cattaaatct ttattataat tttcaattac
    55621 atctattaga ttattacaag atgtttcatc catagctata gaagcaggat tattaggatt
    55681 aacaacgaat aaagctttaa tagatttgtc acgaagtttc tctaattctt tttttgtata
    55741 ttgccatgta tgagcacctt tttcatcaac ttcattagca tttatattta ccactttaaa
    55801 gtcgtaacga ggaaggttag gtatttcaag ataaggtgta aatattggag tcattaaggc
    55861 tatggtatct ccttttttta gaagttcatt tgccattaat gaatcaaata tatagcacat
    55921 agcagctgta gcaccttcaa cagcaaaaat ttctacatca ccatatttac ctgatatatc
    55981 atatttcatt tcttgaacta ggtaatcgtg tactattttt tccatatgag gtaacattct
    56041 atctggaagt ggatagttat caccaataat tccatcagct agttcatgaa caaattcatc
    56101 tccatcaaat ccaagttcat ttataccaaa gtttataata tctctagcta aatatgctcc
    56161 aggcatatct atattttcat ttataaattt aaaaaatctt tctttaatac cctttttttg
    56221 aggcattcca gccaaatgac ctaaatccca attagaacga gtttcagtaa tagcgaaatg
    56281 accaaaagta aaaaaggctt ctctagcagt tgaacaagtc cagtttggat ttccacgacc
    56341 tgcatcaagt aattctctgt tattttcttt tgcagtgtat tttgcaatat ctattaattt
    56401 gtccttaaat tcaaaagggc ttatttttcc atatatgttt ttgatttctt tataagtatg
    56461 atttttcatg ttaaattcct ccttatgtaa taagtttcac atttttataa taaaataaac
    56521 aagttgcaaa aatgattata cttt
//
LOCUS       DAEMIK010000004        55130 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEMIK010000004 DAEMIK010000000
VERSION     DAEMIK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.004"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..1355
                     /locus_tag="KPK87_000194"
     CDS             159..1355
                     /locus_tag="KPK87_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG2536395.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1448..1972
                     /locus_tag="KPK87_000195"
     CDS             1448..1972
                     /locus_tag="KPK87_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG2536396.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1997..2173
                     /gene="rpmF"
                     /locus_tag="KPK87_000196"
     CDS             1997..2173
                     /gene="rpmF"
                     /locus_tag="KPK87_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG2536397.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2380..2937
                     /gene="fapR"
                     /locus_tag="KPK87_000197"
     CDS             2380..2937
                     /gene="fapR"
                     /locus_tag="KPK87_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG2536398.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2948..3970
                     /gene="plsX"
                     /locus_tag="KPK87_000198"
     CDS             2948..3970
                     /gene="plsX"
                     /locus_tag="KPK87_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG2536399.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3996..4982
                     /locus_tag="KPK87_000199"
     CDS             3996..4982
                     /locus_tag="KPK87_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG2536400.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5091..6020
                     /gene="fabK"
                     /locus_tag="KPK87_000200"
     CDS             5091..6020
                     /gene="fabK"
                     /locus_tag="KPK87_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG2536401.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6036..6986
                     /gene="fabD"
                     /locus_tag="KPK87_000201"
     CDS             6036..6986
                     /gene="fabD"
                     /locus_tag="KPK87_000201"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG2536402.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKSNVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6986..7735
                     /gene="fabG"
                     /locus_tag="KPK87_000202"
     CDS             6986..7735
                     /gene="fabG"
                     /locus_tag="KPK87_000202"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG2536403.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7789..8013
                     /gene="acpP"
                     /locus_tag="KPK87_000203"
     CDS             7789..8013
                     /gene="acpP"
                     /locus_tag="KPK87_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG2536404.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8042..9280
                     /gene="fabF"
                     /locus_tag="KPK87_000204"
     CDS             8042..9280
                     /gene="fabF"
                     /locus_tag="KPK87_000204"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG2536405.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9506..11224
                     /locus_tag="KPK87_000205"
     CDS             9506..11224
                     /locus_tag="KPK87_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2536406.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11255..13255
                     /locus_tag="KPK87_000206"
     CDS             11255..13255
                     /locus_tag="KPK87_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2536407.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13606..13851
                     /locus_tag="KPK87_000207"
     CDS             13606..13851
                     /locus_tag="KPK87_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG2536408.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13886..14611
                     /locus_tag="KPK87_000208"
     CDS             13886..14611
                     /locus_tag="KPK87_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG2536409.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14633..15946
                     /locus_tag="KPK87_000209"
     CDS             14633..15946
                     /locus_tag="KPK87_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2536410.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            16152..16691
                     /locus_tag="KPK87_000210"
     CDS             16152..16691
                     /locus_tag="KPK87_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2536411.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16780..18765
                     /locus_tag="KPK87_000211"
     CDS             16780..18765
                     /locus_tag="KPK87_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG2536412.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18989..19957
                     /gene="spoIIIAA"
                     /locus_tag="KPK87_000212"
     CDS             18989..19957
                     /gene="spoIIIAA"
                     /locus_tag="KPK87_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG2536413.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19947..20468
                     /locus_tag="KPK87_000213"
     CDS             19947..20468
                     /locus_tag="KPK87_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG2536414.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20485..20682
                     /gene="spoIIIAC"
                     /locus_tag="KPK87_000214"
     CDS             20485..20682
                     /gene="spoIIIAC"
                     /locus_tag="KPK87_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG2536415.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20698..21084
                     /gene="spoIIIAD"
                     /locus_tag="KPK87_000215"
     CDS             20698..21084
                     /gene="spoIIIAD"
                     /locus_tag="KPK87_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG2536416.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21169..22374
                     /gene="spoIIIAE"
                     /locus_tag="KPK87_000216"
     CDS             21169..22374
                     /gene="spoIIIAE"
                     /locus_tag="KPK87_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG2536417.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22434..23051
                     /locus_tag="KPK87_000217"
     CDS             22434..23051
                     /locus_tag="KPK87_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG2536418.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23063..23617
                     /locus_tag="KPK87_000218"
     CDS             23063..23617
                     /locus_tag="KPK87_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG2536419.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23644..24333
                     /locus_tag="KPK87_000219"
     CDS             23644..24333
                     /locus_tag="KPK87_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG2536420.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24443..24805
                     /locus_tag="KPK87_000220"
     CDS             24443..24805
                     /locus_tag="KPK87_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG2536421.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24890..25396
                     /gene="nusB"
                     /locus_tag="KPK87_000221"
     CDS             24890..25396
                     /gene="nusB"
                     /locus_tag="KPK87_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG2536422.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25380..26450
                     /locus_tag="KPK87_000222"
     CDS             25380..26450
                     /locus_tag="KPK87_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG2536423.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26447..27652
                     /locus_tag="KPK87_000223"
     CDS             26447..27652
                     /locus_tag="KPK87_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG2536424.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27654..27869
                     /gene="xseB"
                     /locus_tag="KPK87_000224"
     CDS             27654..27869
                     /gene="xseB"
                     /locus_tag="KPK87_000224"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG2536425.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27872..28759
                     /locus_tag="KPK87_000225"
     CDS             27872..28759
                     /locus_tag="KPK87_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG2536426.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28789..29226
                     /locus_tag="KPK87_000226"
     CDS             28789..29226
                     /locus_tag="KPK87_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG2536427.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29260..31125
                     /locus_tag="KPK87_000227"
     CDS             29260..31125
                     /locus_tag="KPK87_000227"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG2536428.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31258..32070
                     /locus_tag="KPK87_000228"
     CDS             31258..32070
                     /locus_tag="KPK87_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBG2536429.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNRA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32090..33769
                     /gene="recN"
                     /locus_tag="KPK87_000229"
     CDS             32090..33769
                     /gene="recN"
                     /locus_tag="KPK87_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG2536430.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKKVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33983..34849
                     /locus_tag="KPK87_000230"
     CDS             33983..34849
                     /locus_tag="KPK87_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536431.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDGIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34942..35433)
                     /locus_tag="KPK87_000231"
     CDS             complement(34942..35433)
                     /locus_tag="KPK87_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2536432.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35494..35934)
                     /locus_tag="KPK87_000232"
     CDS             complement(35494..35934)
                     /locus_tag="KPK87_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2536433.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36095..37153
                     /locus_tag="KPK87_000233"
     CDS             36095..37153
                     /locus_tag="KPK87_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG2536434.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37295..38119
                     /gene="spo0A"
                     /locus_tag="KPK87_000234"
     CDS             37295..38119
                     /gene="spo0A"
                     /locus_tag="KPK87_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG2536435.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38532..39653
                     /locus_tag="KPK87_000235"
     CDS             38532..39653
                     /locus_tag="KPK87_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536436.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39671..40546
                     /locus_tag="KPK87_000236"
     CDS             39671..40546
                     /locus_tag="KPK87_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG2536437.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40570..41262
                     /locus_tag="KPK87_000237"
     CDS             40570..41262
                     /locus_tag="KPK87_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2536438.1"
                     /translation="MDSYIGIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41270..42094
                     /locus_tag="KPK87_000238"
     CDS             41270..42094
                     /locus_tag="KPK87_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2536439.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42099..43166
                     /locus_tag="KPK87_000239"
     CDS             42099..43166
                     /locus_tag="KPK87_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG2536440.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43168..43704
                     /locus_tag="KPK87_000240"
     CDS             43168..43704
                     /locus_tag="KPK87_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2536441.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43772..44377
                     /locus_tag="KPK87_000241"
     CDS             43772..44377
                     /locus_tag="KPK87_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG2536442.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44389..45303
                     /locus_tag="KPK87_000242"
     CDS             44389..45303
                     /locus_tag="KPK87_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2536443.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45345..46517
                     /locus_tag="KPK87_000243"
     CDS             45345..46517
                     /locus_tag="KPK87_000243"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG2536444.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKDIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46564..47376
                     /locus_tag="KPK87_000244"
     CDS             46564..47376
                     /locus_tag="KPK87_000244"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG2536445.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47580..48905
                     /locus_tag="KPK87_000245"
     CDS             47580..48905
                     /locus_tag="KPK87_000245"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG2536446.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49118..50197
                     /gene="mltG"
                     /locus_tag="KPK87_000246"
     CDS             49118..50197
                     /gene="mltG"
                     /locus_tag="KPK87_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG2536447.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFLYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50369..51043
                     /locus_tag="KPK87_000247"
     CDS             50369..51043
                     /locus_tag="KPK87_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG2536448.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            51030..52277
                     /locus_tag="KPK87_000248"
     CDS             51030..52277
                     /locus_tag="KPK87_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG2536449.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIIVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52349..54013
                     /locus_tag="KPK87_000249"
     CDS             52349..54013
                     /locus_tag="KPK87_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG2536450.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54110..54751)
                     /gene="sigK"
                     /locus_tag="KPK87_000250"
     CDS             complement(54110..54751)
                     /gene="sigK"
                     /locus_tag="KPK87_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HBG2536451.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
BASE COUNT        22316 a         5596 c        10250 g        16968 t
ORIGIN      
        1 ttatattaaa aggtgatata ttatttatga aaatcgttta atgtatacaa taatatatct
       61 ataacaaaat aataaaagat agagttttat ttagacattt attacaaatc aaaatttatt
      121 acatattatt tataaaaaaa tctaaggagg aaataataat gaaaatatta gtattaaact
      181 gtgggagttc ttcattaaaa tatcaattaa tagatatgaa taatgaagaa gttttatgta
      241 taggattggt tgaaagaata ggaatagaag gttctatatt aaaacatgaa aaagcaggta
      301 gagatgataa atacgttgtt gaacaaccaa tgaaggacca caaagatgca atagcattag
      361 ttttagaagc tgttgctcat ccagaatttg gtgcagttaa agaaatgaaa gaaatagatg
      421 cagtaggaca tagagttgta catgctggag aaaaatttgc aacttcagta gtaataactc
      481 cagaagtaga agaagcttta aaagaatgta tagatttagc tccacttcat aatccagcaa
      541 acataatggg aatagatgct tgtaaagcta tacttccaga tgtaccaatg gtaggtgtat
      601 ttgatactgc tttccatcaa acaatgccta aatcttcata cttatatggt ttacctcatg
      661 agctatatac taaatatggg gtaagaagat atggattcca cggaacttct cataattatg
      721 tatctcaaag agcagcagag atattaggaa aagatataaa agatttaaaa atagtaactt
      781 gtcacttagg aaatggtgct tctatagctg ctgttgatgg tggaaaatgt gtagatacat
      841 ctatgggatt cactccatta gaaggattaa taatgggaac tagatgtgga gatatagacc
      901 cagctatatt accattctta atgagaaaag aaggtttaga tgctgatgga ttggataaat
      961 taatgaataa agaatctgga gtatatggaa tgactggaat ttctagtgac ttcagagata
     1021 tagaagatgc agctaaaaat ggagacgaaa gagctcaagc tacattagaa gcgtatgtta
     1081 aaaaagtaca aaaatatata ggcgcttatg ctgctgaaat gaatggattg gatgttgtag
     1141 tatttactgc aggcgttggt gaaaatggaa aggctataag agcagatatt gcttctaaca
     1201 tggagttttt aggaatgaaa ttagataaag aagctaatga tgttagagga aaagaaacag
     1261 taatatctac tgctgattct aaagtaaaaa tgcttttaat accaactaat gaagaattaa
     1321 tgatagctag agatacatta agattggtaa aataattaaa ttaaacctat gcctatcaag
     1381 taaaaatact tgacaaacaa atgctagatt tgtataataa atttggtgat atgttgaggt
     1441 gaacttaatg aaagttagca tagaaaaaat aaatagaaaa gaaactgaca aaatagactt
     1501 gaatttttgt gaaaaaattg atactattag ttattgtgat gagatttaca aattagtatc
     1561 acctgtaaat ttaaaaggga aagtgtcaaa gactaataaa ggtttgtatc tagatataga
     1621 tgtcaatttt acaattgttg acaattgttc aagatgtctt aaagaagtag aaataccact
     1681 agagtattct atacaaggtt ttttagtaaa agaagaagat tatgatgaag atgagtttga
     1741 ggaatttgat ccttttatat tcgatggtga agaaatcgac cttattgata taatagaaca
     1801 gacattagat tttaatgtac ctcataaagt tctttgtagt gaaaactgta aaggtctttg
     1861 tcaagtatgc ggggcaaact taaatgaaga agagtgttct tgcagtgaaa ttacaaacga
     1921 tgaggaatac atagatcctc gttttgctaa attaaaagat ttatttaatt aaattttgac
     1981 taaggaggtg gcgtaaatgg cagtaccaaa gcgtaaaacg tctaaatcaa acacaaaaat
     2041 gagaagagca gctaactcaa agatggaagc aacaggattt gtaagttgcc cacaatgtca
     2101 tgagccaaaa ttaccacata gagtgtgtcc agactgtgga tattataaag gtaaagaagt
     2161 tgtgtctaag taatagcttc taaaaagcat gtagagtata ctctacatgt tttttttgta
     2221 catataaaaa aataagatga tttactagag aaaataagtg gattttatgc atattaaaaa
     2281 atagaaaaat aataatataa gtattgaaaa tttttgtgtg ttactatata ctactattag
     2341 tagctggtac taattacagc tataaaattt ggagggaaca tgaaaaagaa aagtaaagcc
     2401 caaagacaaa aagagcttat agatatgcta aagacagatc ctttttatac tgatgaagaa
     2461 ttatcaagtc tgtttgatgt aagtatacag accataagat tagatagaat gagtctaaac
     2521 ataccagagc taagagaaag agtcaaatca atagcagaaa ctcagagttc aaaagtaaag
     2581 actttaggag taaaggaaat tacaggagaa atcattgatt tatctgtagg gcgattaggt
     2641 atatctatgt tagaagttac acaggacatg atttactcta agactaatac tttaaaagat
     2701 acgtatattt tttctctagc agattcattg gcaatggcta taatagatgc acctaaggtt
     2761 attatgagag ttgcaaatgt aaaaagtttt aagttgatag aacaacaaga taggcttata
     2821 gcaaaagcag aagtgtatag aaatatagat aaaaagcatt atgtaaaagt tgttatcaac
     2881 aataaagccc aagagcaaat atttagagga aagtttatct ttgaagaatt agattaggag
     2941 ggaaattatg aaaatagtaa ttgatggaat gggtggagat aatgcaccta aatctaatgt
     3001 agaaggcgca gtaaatgcta taaaagaata tcaggttgat ttaataatca caggagataa
     3061 agatttactt gaaaaggaat tttcaaatta tgaatttgat agaaataaat tagagatagt
     3121 tcatactact gagattatag aaaacgaaga taaaccagta aaggctataa gaagtaaaaa
     3181 agattcatct atggtagtag cattaaatct tgtaaaagaa ggaaaggcag atgctataat
     3241 atcagctgga aatacaggag ccttacttgc tggtggatta tttgttgttg gtagaataaa
     3301 aggaatcgat agaccttgct tatgttcagc aataccaaat gtaaagcgag gaatgacact
     3361 tatagcagac tgtggcgcaa atgcagattg taaacctaag aatctagttg aatttgcagc
     3421 tatgagtaat atttattcaa gaaaagtttt aggtcttgaa aatcctaagg tagcattagc
     3481 aaatgttgga ctagaagaag gcaaaggcaa tgatttagtc aaaaggtcat atgaagaaat
     3541 taaaaaatta gatttaaatt ttatagggaa tgtagaagct agagaagtta taaatgcata
     3601 cacagatata atcatatgtg atggatttac aggtaatata ttacttaaat ctgctgaagg
     3661 tgtagcactt tcagttatga gtcttataaa ggaaactttt atggccagta caaaaagtaa
     3721 aataggagca ttacttataa aggatgattt aagaaaatta aaaagtttta tagattattc
     3781 tgagtatggg ggagctccac ttttaggtct aaatggagga gttataaaag ctcatggtag
     3841 ttctgatgct aaagctataa aaaatgctat aaatcaagga ataaaatttt caaagggaaa
     3901 agtcgttgaa gatattaacc aatttataag caaatacaat gaagaaaata aaaataacga
     3961 agatgaataa actcgttttg gaggtttaat atataatgaa tacaaaggct ggaatattag
     4021 gagtaggaag ttatctacct gagcaatcat atgataattt tcacttcgaa aaaataatgg
     4081 atacatcaga tgagtggata agtactagaa ctggtattaa ggaaagaaga tttgccaaag
     4141 agtctgaagc aacttctgac ttagcatcta aagctgcctt aaaagctata gaatgtgcaa
     4201 agttaaacgt agaggatata gagctgatta tattagctac aattactcct gatatgtctt
     4261 tgccttcaac tgcatgtata gtacaagatg caataggagc agtgaatgct actgcatttg
     4321 atatatcagc agcttgttct ggatttgtat atggagtaac tattgcaaag caatttgtgg
     4381 aaacaggttg ctataaaaat gtgcttgtaa taggagcaga aacttgttct aagtttttaa
     4441 attatgatga tagaacaaca gcagtattgt ttggagatgg agctggagca gctgttattg
     4501 gccctgtaaa tgagggtgga atactgtcta cacatatggg ctctgatggt aaaggaaaag
     4561 attgtttaaa agtacctgct ggaggttcaa gacttaaggc aagtaaagag actgtagaag
     4621 ctaatctaca tacaatagaa atggcaggta gtgatgtgtt taaatttgca gttagaaaga
     4681 tggcagaaac ttctttaaga gctcttgaaa aagcaaatct aaatacaact gatatagact
     4741 acttagtgcc acatcaagct aatataagaa tcatacaagc ttcttctaag agattagagc
     4801 ttgatatgaa aaaagtttat gtaaatatag acaaatatgg aaatatgtct gctgcatcta
     4861 taccagtagc attagatgaa gcttatagag aaggtaaaat taaaaagggt gataatgtag
     4921 ttttagttgg ctttggtgga ggtctaactt ggggggcttc agttgtcaag tggactttat
     4981 agtataaaat atctaaaatg atttaattaa caaaattaaa cttatacaca aaataaagtt
     5041 gattaataat aaaaagttta cttttatgat ttcaagtgaa ggagaattgt atgaataaaa
     5101 tttgcaaaat attaaatata aaatacccag ttatccaagg gggaatggca tgggtagcta
     5161 ctgcatcatt agcaagtgct gtatctaatg caggaggact tggcataata gcagcaggaa
     5221 acgcaccaaa agaagctata aagaaagaaa ttgttgagtg taaaaaatta acagataaac
     5281 cttttggagt aaatgtaatg cttatgtcgc catttgttga tgatataatt gatttgatta
     5341 tagaagaaaa agttcaagtt attactactg gtgctggaaa tcctgcaaag tatatggata
     5401 gattaaagga agctggaaca aaggttattc ctgtagtacc tacaatagct ttggcacaaa
     5461 gaatggaaaa gctaggagct acagcagtaa tagcagaagg tactgaaggt ggaggacata
     5521 taggagaact tactactatg gtcttagttc cacaagttgc tgatgctgta aacatacctg
     5581 taatagctgc tggaggaatt gtagatggta gaggaattgc agcatcattt gcattaggtg
     5641 ccagtgcagt tcaagtagga actagattta tttgcagtga agagtgttct gtccattcaa
     5701 actataaaaa cttagtacta aaagcaaaag atagagatgc aattgtaaca ggaagaagta
     5761 ctggtcatcc agtaagaaca ttaaaaaata aactatcaaa agaattttta aagatggaac
     5821 aaaatggagc tactcctgaa gaactggata aaaaaggtac aggagcttta agatttgcaa
     5881 cagtagatgg agacatagaa aaaggttcat ttatggcagg tcaaagtgct gctatggtaa
     5941 aagaaataac accttgtaag gaaattatag aggctatggt aaatcaagca agagagatta
     6001 tgccagcaat agaactgtaa atcaggagga gcaagatggg aaaagttgca ctagtattcc
     6061 ctggtcaagg agctcaatat gtaggtatgg ccaaggattt atatgaaaat aatgctgttg
     6121 ctaaaagtgt aatagatgag gctagtgatg ccttaaatat ggatttaaaa aaccttatgt
     6181 ttaatggaaa tgaagaagaa ctttctaaaa ctgaaaacac tcaacctgcc atggttacac
     6241 atagtgtagc agtattaaaa gcggtacaag ctcaaataga tttaaaatat aatgcatgct
     6301 taggattatc cttaggagag tatagtgcgt tggttgcagc agatgcaatt gactttaaag
     6361 atgctgtatg tttagttaaa aaaagaggta agtttatgca ggaaacagtt ccagcaggtg
     6421 ttggagctat ggcagcagtt ttaggtttag atagaaatgt acttgaaaat gtggttaaag
     6481 atatacaagg aggtattgta gaagtagcaa attacaattc tcctggacaa atagttatat
     6541 ctggtgaaaa tgaaaagata gaagaagcaa tggtaaaatg caaagaagca ggagcaaaaa
     6601 gggcagttaa attaaatgta agtggacctt ttcactcatc aatgttaaaa gaagctggtg
     6661 taaaattggc cagtgaatta gaaaaagtta acataactaa acctaaagtt gatgtggtgg
     6721 caaatgtaaa tgcagattac tataaaaacg aaggtaaaga cctgcttata aaacaagtaa
     6781 gttcatctgt tctttgggaa gattctatag aaagactttt aaatgatgga tatgatactt
     6841 ttatagaaat gggacctgga aaaactttaa aagcatttat taagaagata gcttccaata
     6901 aaaaatcaaa tgtcaatatt tataatatag atggtataga ttctcttaat gaatttgtac
     6961 aaaattatag aaatggagag atataatgat aaatcttaca ggacaagtag cagttgttac
     7021 tggtggctct aggggtatag gaaaagaaat agcaaaaaaa ctagcatctt ttggagctga
     7081 tgtagtaatc aattatactt ctaaagaaga tgaagcacta aaaactaaaa atgaaataga
     7141 aagtatgggg gtaaagtgta cctctataaa atgtgatgta tctaaatttg atgaagtgaa
     7201 tcaaatgata gattctgttg taagcgaatt tggaaaaatt gatatattgg ttaataatgc
     7261 aggcataact aaagatggtc tgcttatgag aatgaaagaa gaagattttg atagagttat
     7321 agatataaac ttaaaaggtg tctttaattg tacaaaagca gttactaaac ctatgatgaa
     7381 aaagaagtat ggaagaataa taaatatgac ttcagtagtt ggaattatgg gtaatgcagg
     7441 gcaaactaat tattgtgcat caaaagcagg tgtaattgga tttacaaaag cttctgcaag
     7501 agagttagca tcaagaaaca taaatataaa tgcagtagca cctggattta tagaaacaga
     7561 tatgactaaa gtactaagtg atgatgtaaa agaatcaaca ctagcaaaca taccaaagaa
     7621 atcttatggt aaaccagaag atgtagccaa tgccgtagca tttttagtta gtgacatgtc
     7681 aagttatata acaggacaag taataaatgt agatggtgga atggtaatgc aataataatt
     7741 taaaaagtag agaaatctat taaaataaaa aattttagga ggtacacaat gtttaataaa
     7801 gttgtagaaa taataataga gcaattagga gtagaagata aagaaataac tatggaaaca
     7861 tcattaatga aggatttaga agcagattca ttagatgcag tagaaataat aatggcactt
     7921 gaagatgaat ttggtataga aataccagat acagaagcag aaaacttcaa atctatagga
     7981 gatatagtta agtatataga agctaataaa tagtcgagct ttaaaaaagt ggaggtatcc
     8041 gatgaataaa agagtagtaa taacaggact tggatgtgtt acgccgttgg gaacaggaaa
     8101 agaagagttc tggagtaata taaaatctgg agtatctggt atagataaaa taactaattt
     8161 tgatgctagc acatatcaaa ctcagatagc aggagaagtt aagaattttc atccagaaga
     8221 atatataagt aaaaaagaat tgaagagatt agataaattt gctcaatttg caatagtatc
     8281 tgctaaatta gcagttgaag atgcaaatct tgatttagat aaagttgata gagaacgttt
     8341 tggtgttata ataggttctg gaataggtgg agttgaagct atagaaactc aacataaaat
     8401 tcttttggaa aaaggaaata aaagagtaag ttcacttttt gtacctatga tgataggaaa
     8461 tatggcagct ggtcaagttt caatattttt aggagcaaaa gggcctaaca ctaatgtatg
     8521 tacagcttgt gcatcaggaa ctcattcaat aggtgatgct tttaaggtaa ttcaaagagg
     8581 tgatgcagac attatggtag cagggggttc tgaagctgct gtaacaggtc ttgcttttgc
     8641 tggattctgt aacatgaagg ctatgtcaac tagaaatgat gaccctaaaa ctgcatcaag
     8701 accatttgat aaagacagag atggttttgt aatgggagaa ggagcaggta ttgttatctt
     8761 agaagactta gaacatgctt tagcaagagg cgctaaaata tatgcagaag tagttggata
     8821 tggtttaact gctgatgcat accatatgac aactccagct gaaaatggag agggagcagc
     8881 taggtctatg aatatggctt tgaaagatgg aaatgttcca ttagaagaag tagattatat
     8941 aaatgctcat ggaacttcaa cttattacaa tgatttatat gaaactatgg ctataaaaac
     9001 tgtatttgga gaaaaggcat atgacttatg tgtgtcatca acaaaatcta tgactggtca
     9061 cttattagga gcatcaggtg ctatagaggc tgttgtttgt gctatgagta tagaagatag
     9121 ttttgtgcct ccaactataa acatacaaga agttggtgaa gatttggatt tagattatgt
     9181 tccaaatcaa ggtaaagaaa aaaatattag atatgcacta tcaaattctt taggatttgg
     9241 tggtcataat gctacaatag tactaaaaaa atatgtataa ataaatatta aatataaaac
     9301 taaaaaacaa gcacttgttt taactgtata aatttatatt agcttaaatt taaagtctcc
     9361 taaattattt taggagactt ttttattaag atttaagtct aggatatgtt gtaaatcatt
     9421 ttgatatatt gtattattaa ttattaagag gttatttata tatgcaagtt ttcattctac
     9481 aatatacaaa aggaggacta aaattttgaa ttttagacta ccaaagaaaa ggttacccaa
     9541 aaagataatt tttatatgta taatacttat tagtattatt ttgtttataa agtatgacca
     9601 aaatggattg aaagaaagag aaattataaa taagggaaaa tatatgtctg aaagccttgt
     9661 ggcagataag gagtatgatg aagcaaaaaa agtggttaag acttctttcg aatctattcc
     9721 agaaaaaaaa tacaataaag tatataatga agtttgggat atgttaaaga ctatttcata
     9781 tgttccagat ggaacgaaga tagtcattga ttctttagaa aagatgagag aatcagatgt
     9841 gaaattatct gatgaatgta gatttaatat agaaaaaaga ttagcatcag tgtatataat
     9901 ggataataat tattctaagg cagtagattt aactgtaaag tctataaagt tggctgagaa
     9961 attggggaat aattatgaaa aagcaagact agatgttgat ttggcgagca tatttttaaa
    10021 gataggtggc tatgaaacag gagagaaatt aataaaggat tcttttaaaa tagatattga
    10081 tgaaggtgaa aaaaatggaa tggttagatt atatgctatt ataaatctag cagaaatata
    10141 tgtagaagag ggagaatatg ataaagctat tgaaataagt agcaggataa aagattataa
    10201 aaagtttgta aatacggata attacaatga ttttgatata atggcctcta taatgcagtc
    10261 aaatgcgtat gtgggcaaaa ataatataca aaaggcaaag acttttttag aaaaagctga
    10321 ttatcttata aaatcagata gaacagttta tatattagat aaagatatgt actattatat
    10381 ggcaatggga aacttagaat ataaaagcga aaattataat tctgcaatag ataattataa
    10441 aaaaagttta gagatatcta ccaaaagaaa gttaacccag gagaaaataa aaaatttaaa
    10501 taagatatta aatatatatg aaaaacaagg aaatatagaa tctttaaatg agtatcaaaa
    10561 gatgttaata aatgaatata aagagaacaa aagtataaga gattcagaga ctagtttcta
    10621 tataattgat aaagtatcaa acgaaagtaa gctatttgaa aaaactaaaa aagaaataaa
    10681 atattataaa atattattta cagtaatttt aattgctata ttagggtcta cttttttata
    10741 caaccgattg aagtatttta aagcacaaaa tttacatgat ggtcttacaa atgtttataa
    10801 tagaaaaagt tttgatatta tgtatgaaaa gtacagagag aaagatgata attttgcact
    10861 tgtgatgata gatattgata atttcaagct cataaatgat aattatggac ataaatttgg
    10921 tgacactgtt ataaagggaa taacaaatac catttgtgtt atgttagaac aagatgataa
    10981 agtctttagg tacggaggag aagaattttc tgtattaata agaaataaat ctggtgatga
    11041 agtagcagat ataattgaca gtataagggt tgcaattgca aataaaaagt ggaatgatga
    11101 tgtgactgtg actataagtg caggtgtagc tcatgtttca gatagcaaag atgtactaga
    11161 agaagctgat aaaaatttat ataaagcaaa acaacttggt agaaataaag tagtatataa
    11221 gtaattatat actatataaa taagaggtat aaatatgaaa caatcaatag ctatagtaac
    11281 tgataaaata actaaactat catctttctt agaagaaaat atacgtttgg ttttaggtaa
    11341 ttatattgat ataaaccact attatctgga agttttaaaa gagaatgata aaatacaagg
    11401 tgattttgta ctagtaatga atgatgatag attaaacagt atgagaaagc atttagtaca
    11461 agatagtaag attatagtga taaggagaac acttaaagaa aatgaaatat atggattatt
    11521 ttcaatacca aatggaactg aagtattggt tgtaaatgat acaaaagaaa ctaccttaga
    11581 aacgataagc ttattttaca aaataggagt aaaaaattta aagtttactc catatattga
    11641 aggaaaaatt tataaaaata tagacatagc tataacacct ggagtaaagg gaaaagtacc
    11701 aagttatata aataaagtaa tcgaccttgg aaatagatat attgatatat ctacctttat
    11761 agaaataata aatggtctta gaatagatgt taaagaaata cgtaaaaatt taatggcata
    11821 ttctgagagt ttaataagtt tagataatgg agttaaagat aattataggc aactgttttt
    11881 aaaaatagaa gaacaagatg ttatattgaa tctatcaaaa gatggaataa tatttacttc
    11941 agcagatggt ataataaaca catttaataa agaagcttta aaaattttag gtataaatga
    12001 aaatccacaa ggcaaaaaca tagaagatat catttctgaa gatttaaata ttttacttga
    12061 agacaaagaa ataattgatg aagtggtatt tacaaataaa aaatatataa atgtaaataa
    12121 aaaaagtatt ttttctatgg ggaataaagc tgggatatat tataacttac aagaaataac
    12181 gtatataaaa aaattagaac aaaatcttac taataaatta agagaacaag gtcagatagc
    12241 aagatataca tttaaagata taaaaactaa aaataaaaat atggtaaagt gtgtagaatt
    12301 ggctaaaaaa atatctaaat cagacctttc tgtattaata ataggagaaa gtggtactgg
    12361 taaagaatta ttatcacaat caattcataa tgcttccaat agaagtaatc aaccgtttat
    12421 agcagttaat tgtgcagcag ttccagatag tctattagaa agtcaactgt ttggatatga
    12481 aaaaggctct tttacaggag ctttaagaga aggaaaaaag ggtctatttg aacttgctaa
    12541 taatggaact atatttctgg atgaaattgg agatatgcct ccattgctac agacaaagtt
    12601 actaagggta ttacaagaaa agcaggttat gcctataggt tcccataatg ttataaacat
    12661 agatgttagg gtcatagcag caacaaacaa aaacttacta aagatgatta gagaaggaaa
    12721 atttagagag gacctgtatt atagactaaa tgtattgcca atagatgtac catcattgag
    12781 gaatagaaaa gaagatataa taacacttat gaatttcttt ttgaaaaaca atataagact
    12841 ttcgccagaa gttataacta ttttagaaaa atataattgg ccaggaaata taagagagtt
    12901 acaaaatgtt gcttcttatg taagtctaat gtgtgatagt atggttttta gagatgactt
    12961 accaccatat ataagggtag aatgtgctaa caattatgaa gatggatttg gttatacaag
    13021 aaaagaattt gaggaaatac tgaggattct atatgaaaga agagagtcat cctctggaat
    13081 tggaagaggg tttataatcg aaaagcttat acaggaaggt attgagattt ctgaaagcaa
    13141 agttagaaag gtgctatctt atttaagtga atgtggatac atatcatcta aatctggtag
    13201 aggaggaagt caaataacct cagaggggat aaaactttat aataatataa attaagctaa
    13261 gtatttgata ttgaaatcac atattttgaa tttagatatt taaataataa taaactttta
    13321 tatttataga gattaattta tattatgtca gaaagaatat tgtaatttat aattaaatat
    13381 taataggttt ttaaaatagg attagatagg taataggtta agttagaata acccattacc
    13441 tatttttatt tgtaagaaaa gtgtacatag aattagaagt aaaaaataca actaataaaa
    13501 ctattgaaaa tactagataa ataataaata aaaataatta tagattttgg catacatatt
    13561 gctaattata gagataagac gacaaaataa ttgggaggtt taatgatgaa atacaaaaca
    13621 acagggactt gtgctacaga aattgaattt gaagtaaaag aaaataaagt tacaaatgta
    13681 aactttatag gtggatgtga tggaaattta aaagggctaa aagtattagt tgaaggcatg
    13741 aatatagaag atgtaataca aaagttaaaa ggaatagagt gtaaaacaaa gccaacatct
    13801 tgcccagacc aactgtctct agcattggaa aattatatga atataaagta atacataaaa
    13861 caatcgatta gttaggagtt taattatgaa accaataata gggattttag gaaatttaat
    13921 aataatggaa aatggtatgt ttccaggtct tgaaaggtca tatgtaaata atgattatat
    13981 aaatgcagtg ctaaaaggtg gcggtagtcc agtaataatt cctgtaaata cagataaaga
    14041 agttataaaa aaacagattg aaatggttga tggagtctta atatctgggg gatgggatat
    14101 caatcctcaa ctgtatggag aagaaacaag agaagaaacg acatttatat atccagaagt
    14161 ggatgaattt gatttaattg caataagtat agcattagaa cttaaaaagc ctatattagg
    14221 tgtatgtagg ggactacaaa ttttaaatgt aagtttagga ggaactttat atcaagataa
    14281 taatcttata gaaggaagtt atataaaaca tacacaatct tctaaaagac atgtagctac
    14341 tcataaagtt gatgttaaag aaggaagtat tttagaggga attttaggaa aacaattact
    14401 tacaaatagt tatcatcatc aaagtgtcaa tcagcttgga aaaggattaa aagccatagc
    14461 atactcaaaa gatggtataa tagaagctat tgagaaagag gatgaaaatt ttgtagtagg
    14521 agtacagtgg catccagaaa tgatggtaga ttactgtgat aaaatggaaa aactatttaa
    14581 atattttata aatatttgtt caaatttata atataccttg gggggattga acatgaacga
    14641 aaagaataaa atgggattaa taagtattat acttttaggt attaatgcag tagtaggagc
    14701 tggagttttt ctattaccag gagatgcaat gaaatcattt ggtgtagcaa gtatatttgt
    14761 atatatattt gatatgttgt tagttttatc tatggcattc tgttttgctg aggttgcagg
    14821 taaattcaat aaaaatggtg ctgcctatgt atatacaaaa gaagcttttg gagatttttg
    14881 tggttttgaa gtaggtctta tgaaatgggt aataggatgt atttcttggg gagctttaat
    14941 agtaggtttt cctacatcat tatcagcagt atgggctcca gcaggtgaac cacatataca
    15001 aaaaattata attgtagcca tgatagttgg gcttacaatt ataaacttat taggtgtatc
    15061 tctatcaaaa attgtacaaa atgttataac tgtaggtaaa ctaattccat taatactatt
    15121 tataggaata ggtatatttt ttataaaagg agttaatttt acttcttcaa caatggtacc
    15181 tccaggagct ggagcaacag agtttggagc agcagcactt ttaatgtttt attcatttac
    15241 aggatttgaa tctatagcag ttgcagcaga agatatggag aatccacaaa aaaatatacc
    15301 aatagcaatt ataagtgtaa tagtaattgc atcaataata tatatattaa atcaagttgt
    15361 atgtgttggt atactaggag attcgttatc tagtacatct acaccagtgg cagatgctgc
    15421 aagaatatgc tttggaaata tgggagctgg attggttacc tttggaacct tggtttctgt
    15481 tggtggaatt tgtatgtgtg gtgcttttgt aaatccaaga agttgtgtag ctttagctga
    15541 tgataaaatg ttaccgagaa tatttgcaag aaaagataaa aaaggtacac catatgtagc
    15601 gataatagct actatgctaa tcactatacc aatagctcta tctggaagtt ttgctgaatt
    15661 agcagcaata agtgcagttg ctagattcat acaatacata ccaacatctt tatccgtatt
    15721 agtgtttaga aagaaaagac cagaattagt aggtactttt aaaactcctt ttggagcagt
    15781 gattcctcta attgctgtat gtgttggaac atggttatta ttccaagcat ctatgcatca
    15841 attaattatg ggattaggag ctttggcaat tggagtacca ttatacttta taatgaagtc
    15901 ttataataga aaagtctatg gtgaaaattt aaagaaaata gtttaattat gttataacag
    15961 tttattgagt taaaattaga cattggcaga aaattaaata caaataagta tctataaggc
    16021 tatattaaaa tattattaat tttttatttt aatatagcct taaattgtta tcaataagtg
    16081 aaaaatattg taacaataga agaacaatag ttttatgttt atagacaaaa aacttattag
    16141 gaggtaaatt tatgccaaca ataacattaa aaaatggtgt agacgtactt attagagaag
    16201 gagtaaggga agatgctcaa agcattatag atttttataa tgaagtgggt ggagaaactc
    16261 attttctgtc atttggaaag gatgaatata aaatatcttt ggaggaacaa gaaaatgcta
    16321 tagaatcagc aaaagcatct gataattctg ttaaactgat tgcatttata gatggtgaaa
    16381 tagttggaat tgcaacgatt gattctaatc aaaaagcaaa aggaaagcat gttggagtat
    16441 taggaattgt tgtaaaagaa aaatattggg gaattggatt gggtaaaagg cttatgttag
    16501 accttataga atggtgtaaa tcaaatggaa taaccaagaa gataactctt gtcactaatg
    16561 aagaaaatta taatgctata ggattataca aaaaagttgg atttgaagta gaaagcatac
    16621 taaagaaaga atgttattat aatggtgttt atactgattt aataggaatg tcacttttat
    16681 tgggaatata attagtgtac actttatctc gaaaaaagta tattaagtat ggtataatta
    16741 aaaaagaata taacttaata taattttgga ggacgaatta tgaaaaatct gtgtaaaata
    16801 tcagatgatt atttaaacag caagcttaag tatttaaagt tattatcaaa acaatatcca
    16861 agtatatcaa aagcaagtac agaaataata aatttagagg ctatattaaa tcttccaaag
    16921 ggaacagagc attttataac agatgtacat ggtgaatatg aaccatttgt acatgtatta
    16981 aaaaatggtt ctggtgtaat aaagagaaaa atagaagaat tgttttcaaa tacaataaga
    17041 gacagcgaaa aaaaaatgtt agcaactctt gtatattatc ctgaacaaaa gttagaccta
    17101 ataattaagc aagaggaaaa tatagatgat ttttatagaa taaatattta tagacttata
    17161 gaactttgta aatatgcttc tagcaaatat actaggtcta aagttagaaa actgttacca
    17221 gaaaatttta aatacataat agaagaactt ttgcatgagc atgttaaaag tgaacataaa
    17281 gaagagtatt ataaaagtat agttgaaaca atagttgaca taggaatagc taaggagttt
    17341 ataattgcta tttctacagt tatacaaaaa ttggttgtag atagacttca tgtaatagga
    17401 gatatttatg atagaggtcc aagaccagat ataattgtcg ataagcttat tgaacatcat
    17461 tgtgtagata ttcaatgggg aaatcatgat atattgtgga tgggtgcagc ctcaggtgaa
    17521 aaaacttgta tagcaaatgc actgaggata tcagctagat atgcgaattt agacattgta
    17581 gaagacatat atggaataaa tctgttgcct ttggccactt ttgctataga aatgtataaa
    17641 gatgaccctt gtaaagaatt tattccaaag gttaatgacc aaagtgtaac aacaacagaa
    17701 aagtctttaa tggcaaaaat gcacaaagca ataagtataa ttcaatttaa gcttgaaggt
    17761 gaggttatca gacgaagacc tgaatttgaa atggaacata gactcttgct taatatgata
    17821 aactatgatg aaggtacgat taatttaaaa ggtaaaacat ataaattaaa agatacttat
    17881 ttacctacaa tagacaaaaa agacccatat aaattaacta tggaagaaag aaatgttatt
    17941 gataaattag tatcttcatt tagaggtagt gaaaaattac aaaaacatgt ttcatttttg
    18001 ttttctaaag gaagtatata tttaaaggct aactcaaatt tactgattca tggatgtgta
    18061 ccgttaaatg aagatggaag ctttatgtct atgaatataa tgggaaaaga gtataaaggt
    18121 aaggctctta tggataagat ggagtctcta gcaagagaag gatttttctt taaagataag
    18181 gcagaagaaa aactgtatgg catggatatt atgtggtatt tatggactgg gaaatgttca
    18241 tcattatttg gtaaagatga tatgactaca tttgaaagat actttatagc tgaaaaagag
    18301 actcataaag aaaataagaa cccatatttt aaacttagag aaaatgagat ggcttgtaaa
    18361 agattatttg aagaatttga tttagaactt gatgaatctc atataataaa tggtcatgtt
    18421 cctgtggaga gtaaaaatgg agagagccca ataaaagcta atggtaaaat tcttgttata
    18481 gatggtggtt tttctagggc ttaccaaaaa actactggta tagctggata cactttgata
    18541 tacaattcta gaactttgca attagtatcg catgaaccat ttaattctgc tgaagaagcc
    18601 attgcaaatg aaagtgatat cttatcgaca actgttgtag tagagcataa ggcaaagaga
    18661 aaaatggtga gagatactga tgaaggagta aagatacaag aggaaataga agatttaaaa
    18721 ctacttttaa tggcttataa gaaagggcta ataaaagaaa tgtagatgtt atagaggcac
    18781 ttcatgattg aagtgtcttt ttgttaatac ttattaaata tattataaag attaataata
    18841 gtagtttata ttatacttac taagtgaatc caagataaat gggttcactt tttttattta
    18901 taaacattat taaaaaaata tagatgtttt ggttatattc cttgtccttt ataaatagaa
    18961 ttatataaac aaaggaggag gacaagagat gaataaactt tctgatgaaa taataaactc
    19021 tctttctaca actataagag aaaaggtaga aaaagtctca aacaataatc tgaacataga
    19081 agaaattagg ttacgctcac aaaaaccatt gatactaaat gcaaactcta aagattattt
    19141 ttacaatcaa aaaactatga cacttgattt aaatcaacaa aactcatatg tagtaacaag
    19201 agaagatgta gagcaaactt ttcaaataat atgtaaatat tcaatacatt catttatgga
    19261 tgacataaaa aaaggattca taactttgag aggaggtcat agagtagggt tagttggaaa
    19321 agcaatagta gaagatggac aagtcaaaaa tataaagcat atatcatctt taaatataag
    19381 ggtatcaagg gaaattattg gatgctcgga taagatttta agtcatatca taaagggaaa
    19441 aaatcagatt aataatactt tgataatatc accacctcaa tgtggaaaaa caactttaat
    19501 aagagatata gtaagaaatt taagtaatgg taatgaagat tatggattta aaggtttaaa
    19561 agtggcttta gtagatgagc gtaatgagat agcaggagct tatctaggtg ttccacaaat
    19621 ggatgtggga ataaggactg atataataga aacttgccca aaagacttgg gtattacaat
    19681 gcttttaagg tctatgtcac caaatgttat tgtgacagat gaaattggaa gtgaaaaaga
    19741 aataaaggca ttatacacag cattaaatgg aggtatagga ttaataacta ctgtgcatgg
    19801 agattcaata gaggatatac aaaatagaaa agaattaaat agacttctgg ataaagagtt
    19861 atttaaaaag gttataattc tttctgcaaa aagaggagca ggaacaattg aaaaaattta
    19921 tgatttagaa gaaaagagat ggtattttgc aaattaaaat aattattata gctttcttga
    19981 taggaagtag ttatttgata ggtgaacaaa tatacaaaac ttatactcgt agacataaac
    20041 aattaaacga tttaataaga gttttagaaa ttttgaggat ggatttatct tttggacttt
    20101 atacattaga agaaatcttt aataggatag gtggaaataa agagttttgt ttttggaaat
    20161 ttttctatca aatatcagaa ggacttcaca atgaacaaag taaaactttg gaaataataa
    20221 tatcagaaaa tattgatgtc ttaagtaaag aaacttatct aggcaataaa gaaattgagg
    20281 aactgaaaaa tctaatactt actttaggga aaagcgatat agagtcacaa caaagaatga
    20341 tagatttatc tatagaaaat ttgaaaaaac aaacctatga gacaaaggaa gatataaaca
    20401 aaaaaggcgt attgtataaa aaattagtga cattcatagg tataggtata tgtattatat
    20461 tgatttaata tggggaggtt tattatggaa atatcattga tattaaaggt tgcaggtgtc
    20521 ggtattctaa tatcagtatt gaatatgata ttggaaaaaa cagatagaaa agattgggca
    20581 ggtcttacta cattagcagg tgtaataatc gtattgggta tggtaataac agaaataagt
    20641 gacttgttta atactgtaag aacaatgttt caactttact aaagaggagg gagtaatttg
    20701 gaaataatgc aattaatagg aattgcaatc atttcaacta cactctgttt agtaattaaa
    20761 aaagatagac cagagatagc aaatttcata gctataataa ctggagtaat aatactattg
    20821 tcagttatgt ttaagctaaa ttttatagta gatagtatac aagatttggc taacaaggct
    20881 aacatcccga ctatgtatat atcattaata ataaaattaa ttgggatagc atacttaatg
    20941 gaatttgcga tacaactttg taaagattgt ggggaaggaa acattgcttc aaaactagaa
    21001 tttggaggta aaataatagt tatgtcaatg tctttcccta tacttttgtc cattgtagaa
    21061 atggtggtga atataattcc atagatgtgt atcttggaat gaatataagt atattagaga
    21121 gctttcgttg tattgaggtt tatagagtga agagaggcat ggttgcttat gaaaaaaaga
    21181 tttttaccaa tgattatagg ttttttattt actttctttt ttgtaaatat ctttgctatt
    21241 atgatatttg ctaatgaagt accaagtagt gaagataaag aatatggtga aacaaaaaat
    21301 agcatagata agtatataga tggtcaatta gacaagctag atataaatga gattcaagac
    21361 tatataaata aagaaatagt tattaatgat gttaatttaa aatcatttgt aaaagattta
    21421 attagtggag aaaaaaatat cttagattta ttcaataaag atggattgaa gatattgatg
    21481 tttgatgaat ttaaagcaag tttaaaggta gtagcagtaa ttttggtact ggctctgcta
    21541 tcatctattt taaagagtct ggaaaactct ttttcatcag gtgcagtaag tcaaatagca
    21601 acatatataa tatttatcac aatggtatca cttacactta ttggttttaa agatgtatta
    21661 cagatttgct atgatgcaat agaccatact gtaggtctta tgcaagttat aatgccaata
    21721 ttaataacat ttttactttt aattggattc ccaataacgt cgactacttt gaatcctatc
    21781 ttcataggag gagtaacatt tataaatgta ttttttaaaa attttctatt tgtatctatt
    21841 actgttgcat ttggaatttt aataataaac aatttatcaa aaaatataag gctaaagaga
    21901 tttttctcct ttgtaaaaca aataaattat gtttctattg gagctatgtt tactgtttac
    21961 ttaggtcttg tatccataca aggactgtat gtaaccagtt ttgataaatt cagtgtaaag
    22021 actgctaaat ttgctatagg taattttatt cctgttgtag gtggatttgt atctgactct
    22081 gtggatattt tattatcttc atctcagctt ataaaaaata tatttggagg aatagggctt
    22141 atcttacttg ttggaatttg cttacttcca gtaataaaaa ttttatcagt aatagtagtg
    22201 tacaaattag cagcaataat tgtagagcct gttggagagg atggaatatc taatttttta
    22261 aatgaagtgg caaatttaat gataataatg ctagcatcag taattgctat aacagtaatg
    22321 ttctttgtaa ctgtagctat cttgacctcg ataagtgtcg taagtcaagg gtaatttata
    22381 tatcagtgaa aaataattac atttaatcta atgtttttag agaggaggat gctatgttag
    22441 agggcataaa ggcatggata gttagtgttt tgataggagc atttatagta aatatagtag
    22501 atatgatttt accaagttct aagataaaac cttatgtcaa cctagtttta aactttatgt
    22561 ttgtatttat tgtaataact ccagtagtag gatttttttc aaaagatatg agtttagaag
    22621 atagaatatt aaaatctatg ggaaactaca acaagcagta tgtagatagt acaaatgctt
    22681 tggcaaaaga gactggaaat aatagtctat ccaagggata tgaggatggg ttaaaagaag
    22741 tacttaaatt aaaacttgat gaatatggtt atgatttaga agatatagag cttaatgggg
    22801 caaatataaa taatataaaa ataaaagaaa agaataatag tgcaaaaagt aataacagtt
    22861 ccaatgaaga aaataaaaat aatagtacta aaagagagga agaaaacata aactctaatg
    22921 acaaagaaaa ttctaaacaa gtcttcaaaa aaggtacaga gtatgggttg aacctaaatg
    22981 aagaaaaatt aaaaaacgac ttaataaagg tgctggatgt ttctatagaa gatatacaaa
    23041 ttgataaata ggagttggga gaatgttgaa aaacttaaat gacaaagata agagaaaggt
    23101 atattcacta ataactatag caggaatatg tgtggtttca ttagtattat tatcttgttt
    23161 tcctagtagc aaaagcgata aacaagttgg aaaaacagag actaataaaa atgctgaaaa
    23221 gcaagtcact aaagaacaag aaaaagatga tttagagtca aaattaacag caatactatc
    23281 taaaattgat ggtgcaggag acgttgatgt aatggttaca tttgaatcaa gtgaagaaat
    23341 acaacctgct tttaattcaa ataatacaac agaaacaaca gaggaaaaag atgcacaagg
    23401 tggagaaaga acagttacga cttctagtga aaataaaaca atgataactt caaattcaag
    23461 tgaccctgta gttataaaaa ctacagagcc aaagataaag ggagtgatag tggtagcaag
    23521 tggagcaagt gacccaagtg ttaaagagac actttatagt gcagtacaaa catcattaca
    23581 agtagctggt catcaagtag aaatatattc taaataaata catataaccg gggggataaa
    23641 tgaatgaagt ttaattataa gggaagagga tttgtaataa taactttaac tgcaatgtta
    23701 gtggtagtag gaacagtgaa ttatcaacta agtaagaaat ctttattaga aacatcaaaa
    23761 gagtttaagg catatgaaga agcacagttg caaaaaaata ctgatgagag tgatagtagt
    23821 aagacagagg attctagtaa tatagataaa caagatggta aggaaagtgc tgatattgat
    23881 atagttgata gcaaagctag taaagtaaaa gaaaaggcaa ctgagacaag taaggaaata
    23941 aaagctcaat tatcatcaga gaaaaatatg aaaaaggcat cttatatttt agatatgaaa
    24001 atgaatagag aaaaacaaag aaatgagtta gttcaagatt taaatgaaat gataaataat
    24061 ccatctacaa cagaagaatc tagaaaagaa gcttctaata tgaaattaaa tatagttaaa
    24121 attcaagaaa aagaacttca aatagaaaac cttttgagta caaaagggta tgaagaagcg
    24181 ttagtttata taagcgataa caaagttaat gtagttgtaa atgaagctaa attagaaaag
    24241 aaagatgcag caaaaatatt tgatttagtt gcagaacaag ccaatgtgaa gtatgaaaat
    24301 attaaactta caaataataa tagtaataag taaagtataa acctcagaat taactgaggt
    24361 tattttcttg caaagtagac ttgtatttgg tattattata cttataaaaa tagttcctta
    24421 aaatcaggag gtaagtttta atatggaaga taataaattt ggacaagtaa aaatatctaa
    24481 tgatgtaata gctacaatag ctggtttagc agcattagaa gttgaaggca tagaaactac
    24541 agcaacatta acagataagt tgttaaaaaa taatggagta aaaatacaaa tcgaagaaga
    24601 ggatgttaat ttagatgtaa tggttacgat aaagtatgga atgtcaatac cagatacagc
    24661 ttttaaagtt caagaaaatg taaaaaatac tgttgagaca atgacaggac tgaaggtttc
    24721 tcaagtaaat atacacattc aaggaatcag ctttaagaag gataaagttg ataaagaaga
    24781 agcaaaagca actaagaaaa attaatatta gccctctgaa aagtcagagg gttttttgct
    24841 ggtaattgat tttatatgga taattaaact taaaggagaa ttttgattga tgaaaaaaga
    24901 tagggcacaa aagagtacaa caagagaata tataatgaaa cttatatacc aaataaatat
    24961 taataaagaa gattttgaaa ctttagaaga taaagtagat aattttttaa aagataattc
    25021 agaacatatt ataaatagat ataaagaact tgcacttcaa tactcaaaaa atacaaattt
    25081 aaagttagaa gatactgaaa ttgaagatgt tatagacaaa aaatatataa atacagtatg
    25141 caaagcattg aaagaaaatc atgacaaaat agatgagtta ataaataagc atgctaaaaa
    25201 ctggacagta gatagaatgc caaaggtaga tgtttctata cttagattgt ctgtgtgtga
    25261 aatactttat ttagacactc ctaataaagt atcaataaat gaagcagtag aacttgcaaa
    25321 aatatattgt gatgataagt ctcctaaatt tataaatggt atattgggaa gtgttgttga
    25381 tgaaattggg aaataatata ataattggaa ttgatactag ctgctatact acttctatag
    25441 cagctatctc tttagataag aaagttatat tcaatgaaaa aataatgtta gaagtaaggg
    25501 ataattcaaa aggtttaagg caaagtgaag ctgtatttca acatatcaat aatttaggga
    25561 ttcttagtga taggataaaa tcttttaaag ataagtttaa tgtagaagga gtttgctcat
    25621 caaaaaaacc gagacctgtt gaaaactcat atatgcctgt attcaatgta ggtcataatt
    25681 ttggaaaatt attgtctagt atatatggtt gtaggtttta tgagactact catcaagaga
    25741 atcatattga agcaagttta ttaaatagta aattaaaaaa taataataaa tttatttctg
    25801 ttcatatgtc tggtggtact acagagatac ttttaacaag caaacaagat agtcatcaca
    25861 atgtatgtga tactaattta ggtaaaattg ccaaaataag tattaaaaaa gatgataaaa
    25921 gtaagttata caataatttt ggatataata tagatataat tggaggaagt aaagatatta
    25981 gctttggtca acttatagat agagtaggaa ttaagcttgg atataaattt ccttcaggaa
    26041 agtatttgga tgaaaatgct ttaaattgta atcttaaaat agaaagtggt ctgaaaactt
    26101 ctgtaagaga tgggtatatg aatttatcag gattagaaaa tcaggtaaat aagattataa
    26161 atgataatgg agataatact aatcaaaaag aatatatatc taaattagta ttagattcag
    26221 tagttagaaa tatgtttaaa tctctagtgt atttatgtga aacttataat gtaaatgaag
    26281 tagtatttgc tggtggagtt tctgcaagta agtatattct tagagaatta agcatgaagt
    26341 taagaaaaaa gcatatagaa gcttatttta cagaaccaca atattcaact gataatgctg
    26401 tcgggtgtgc tataatagga ttgaataact ttttaggaga aagagtatga aactaagagc
    26461 tttagatata agtgaagcaa actcatatat taaaagaatt ttaataaacg acccaatact
    26521 ttctaatctt aaagttaaag gagaaatttc taattttaag gttcatagta gtgggaatgt
    26581 atatttatca cttaaagatg aaacatcaaa gttaaactgt gtaattttta aaagtaattt
    26641 taatcgcaat ttaaaattag ataatggtgt aaaaattata gctaatggat atatatcagt
    26701 ctatgaaaga gatggtgcgt atcaattata cattaatgaa attgaaatag agggaatagg
    26761 aaatttacac atagagttta atagattaaa ggaaaaactt aataaagaag gattatttga
    26821 ccctaaatac aagataccta tacctaaaat gccaaactca attggagtta taacatctcc
    26881 aacaggtgct gttataagag atataataaa tgtaataaaa agaagatatc cgaaagttaa
    26941 tataaagctt tatccagtaa cggttcaagg agataaatca gctgaagaaa tctgtgaagc
    27001 aattagattt ttcaatcata tgaagaatgt tgatacactt atagttggta gaggtggggg
    27061 ttcaatagaa gaactttggt cttttaatga tgaaatggta gcaagagagg tttttaattc
    27121 tcaaattccg ataatatcag cagttgggca tgaaacggat tttactattt gtgactttgt
    27181 gtctgatatg agagccccaa cgccatcagc agcagctgag attgctactc catcattgga
    27241 tgatataaac tataaacttg gaaacataaa aagtaggatg agtaagtctc ttacaaatca
    27301 aatagagcta gaccaatata ggttagaaac agtatttaat aagataaata actatttaga
    27361 ttcgtacaca ataaaagata aagttataca attagacaaa atatatgata aaataatttt
    27421 tggaatagaa aataatttaa aattagaaga tgaaaaatta gtaaaaattg gagcattact
    27481 ccataattta agtccattag ctactatgga tagaggttat agtataactc aaaaaaatgg
    27541 aaaagtaata aatagtataa aaggattaaa aataaaggat agtatagata tagtattaaa
    27601 agatggaaat ttagaatgta tgatagataa aattgaaaat aaagagggat agtatgaatt
    27661 taacctacga agaagcttat aaaagattag aaagtatttt aagtgaatta gaatctaaaa
    27721 atgctagctt ggatgaatct ttaagcctat atgaagaagg tataagcctt tataaacatt
    27781 gtaataagct tttagatgat gctaaattga aaataagtaa atttaatcaa ttgggaatag
    27841 aagaagactt taaaatagag gaggaataaa attggaattt aaacaatgtc taaaagaaaa
    27901 agcaagtttt gtagaaaaag tacttaaaga atatatgcct aaagaagaag ggtatcaaaa
    27961 aactgttatt gaagctatga actatagctt aagtgctgga ggcaaaagat taagaccaat
    28021 acttacattg gaggcttgta aaatagttgg aggtaatgaa gatgaagcta ttccttttgc
    28081 tattgcaatt gaaatgatac atacatattc actaatacat gatgatttgc cagctcttga
    28141 caatgatgat ttaagaagag gaagaccaac aaatcataaa gtatatggag aagctatggg
    28201 gattttagca ggagatgctt tgttaaacta tgcttttgaa gtaatgcttg caggctcaat
    28261 caataaagaa aatccagaaa agtatctaaa agctataaat gaaattgcca aaggtgctgg
    28321 tatatatgga atgataggtg gtcaagtagt agatgtagaa agcgaaaata aacaaattga
    28381 aaaagaaaaa ttagattata tacatatgaa taaaactgct gcaatgatgg taggctgtat
    28441 gagagcagga gctactatag gtggagcaaa ttctgaacaa atggaagaaa taactaaata
    28501 tgcaaaaaat atcggattat cattccagat agtagatgat atactagaca tagtaggaga
    28561 tgaagctaag ttaggtaaaa aggttggaag tgatatagaa aatcataagt caacataccc
    28621 atctctttta ggtttagata aatctaaaga aattgcacat aatttgattg atgaagcaaa
    28681 gaaaagtata gaaaaattat ctgatgatgt agatttttta aagggattgg ctgaatatat
    28741 aatagataga gaatactaaa atatacagct aaaatcgagg agggaaaaat ggattttttt
    28801 tcggagattt ttaacaatgg ggctttggga ataagtctga ttgcttgttt cttagcacaa
    28861 tttataaaaa tatttactgg taaggaaaaa agaattgagt tatcaagaat acttatttca
    28921 ggaggtatgc ctagttccca tagttctttt gttacaagtt tagctacagt ggtaggtatt
    28981 gaaaaagggt ttaattctac tgattttgct ataattaccg tgcttgcctt aattataatg
    29041 tatgatgcag caggtgttag acgggctgtt ggtaaacaag ctactatatt aaatcaaatg
    29101 gtggcagata tacagcatgg aaagcatata gagcagaaga aattaaaaga attaataggt
    29161 catacaccac tagaagtttg gtttggagct ttacttggaa tagtaacagc tttaatattg
    29221 atgtaaattg aaaatttaca gaataaaggt gaggataata tgtataaata tttagataaa
    29281 gtaaattctc caaaggatat aaaaaatatg agtatagaag aaatggattt acttgcaaag
    29341 gatataagaa agttcttagt aaaatctgtt tcaaagacag gaggacactt agcttctaat
    29401 ctaggagtgg ttgagttaac attagcactt cataaagtat ttgatagccc taaagataag
    29461 attgtatggg atgtagggca tcaatcttat gttcataaaa tagttactgg aagaaaagat
    29521 tgttttgtat ctttgagaca gtttaatgga ctaagtggtt ttccaaagga gaatgaaagt
    29581 cctcatgata tttttgatac aggacatagc agtacatcta tttctatagc tacaggtatt
    29641 gcctgtgcta gggatataaa aaaagaaaac tatagtgtta tatctgtgat aggagatggt
    29701 tctataacag gaggtatggc acttgaagct ttaaatcaat taggatatat agataccaat
    29761 atgatagtaa tccttaatga taatgagatg tccatagata aaaatgttgg aggaatgtct
    29821 aaatatctgt caagtattat aagaaactca acagttgaga aaatgacaga tgaagttgat
    29881 aaaattttaa atgttacaca aacaggtgaa attttatcta agactgcaca tagatttaaa
    29941 gataaactaa tgtatagctt ttctcctcag gactgttcat tttttgattc attaggtata
    30001 agatattatg gacctataga tggccataat actaaagaac taatagatat attgagaaaa
    30061 gctaaacaca aaaaagggcc tgttctttta catgtaatta cgaaaaaagg aaaagggtac
    30121 agatttgcag aagaacaacc tgataaatat catggagtat caaagtttga tataaagaca
    30181 ggtgttacat ctgcaaaagt taagtctatg tcaattagtg ttggagaaaa acttgttgat
    30241 atggctaata gtaatgaaaa tatagtagca ataacagctg caatgccatc tggtacaggg
    30301 ttaaacctat ttgaaagtgc atatccaaaa agatattatg atgttggtat agctgaacaa
    30361 catgcaacag gatttgcagc aggtcttgca aaaaatggta tgaaacctta ctttgctgtg
    30421 tactcatcat ttttgcaaag ggcatatgac caagttattc atgatgtatg cataacaaaa
    30481 aagccagtta cttttcttat agatagagct ggattagttg gaaatgatgg agaaactcat
    30541 catggtatgt ttgatttaag ttacttaaat tctattccaa atattgtagt aatggctcca
    30601 aaagacacaa gagagatgga gcttatgatg gatttgtcat taaaattaga ttgtccacta
    30661 gcaattagat acccaagagg aagtagttat tacctagata agggagagta tggagagatt
    30721 gtactgggta aatatgaggt attagatgac ggtcaagata cagtcatact ttgtattgga
    30781 agtatggtaa aacatgcttt agaggctaaa gaaatattat caagagaagg aataaatccg
    30841 acaatagtta atgctagatt cttaaagcca attgatgaag gtatgttaaa agcattatta
    30901 aaaaatcata aaaatgtagt tactatagag gataatattg taactggagg atttggaagt
    30961 agaataaata agtttattat agataatgaa tataacgtaa atatacttaa tatagcaata
    31021 ccagaagaat ttgtaaaaca tggcaatata gatgaattat atgattttgt aggattatcg
    31081 ccaaaaagta ttgctgataa aataaggaaa ttagttattg agtaaaaatc taatgtagat
    31141 attgtaaatt aaaatttata taaaatatag aatttatcaa ttagaataaa tatcaagtgt
    31201 gttacttcaa gctactttag ttatcattaa agtagctttt aacagaaagg taattgtatg
    31261 aaaaaaagaa tagatttatt attagtagaa caagggtact ttgaaagtag agaaagagcc
    31321 aaaaaagcaa taatggctgg attggttttt gttgataatc aaaggtgtga taaggctggt
    31381 acagaagtaa aagaagattg tagtatagaa gtaaaaggaa atccaatacc atatgtcagt
    31441 agaggtggtt taaaacttga aaaagcaatg aaaaactttg atttgactat tgatggaaag
    31501 gtatgtatgg atataggggc atccacaggt ggttttacag attgtatgct caaaaataga
    31561 gcaataaaag tattttctat agatgttggt tatggtcaac ttgcatggaa attgagacaa
    31621 gatgataggg ttgtgtgtat ggaaagaacc aatataagaa atgtgacaat agaagataca
    31681 aagcagtttg ctgattttgc gtcaattgat gtgtctttta tatcgttaaa gttagtactt
    31741 ccaaaggcaa aggagttagt acgtcatgat ggggaagttg tcgctttaat taaacctcaa
    31801 tttgaagcag gaagagaaaa agtaggtaaa aaaggagtag ttagagaaaa atctacacat
    31861 atagaagtta taaaaatgat ttctgatttc tcagtcgaaa atggatttga gattttaggg
    31921 ttagattttt ctcctattaa aggtcctgaa ggtaatatag aatatctaat tcatttgaga
    31981 aatggaaatg aaggatatga atttgatggt gagacataca ataacaaaat agtagaagtt
    32041 gttgaagctt ctcataattt agacaaatag gttaaaaggg ggcttagatt tgatcctcga
    32101 attatatatg aaaaattgtg ccttagttga agaactaagg ttgaatattg ataaaaactt
    32161 aaatatactt acaggtgaaa ctggttcagg aaaatctata ataatagatg cacttggact
    32221 ttgtttaggt gataaatatg ataggtcttt tttaagaaaa ggaacagata agggacttgt
    32281 tgaggctgta tttttttcag acaatagata tcttaaaaaa atattagaag aaaatgatat
    32341 aagtatggaa gatgataatt tattggtaat aactaggctg atatactcag atggtaagag
    32401 tactgctaga gtaaatggaa ggactgttaa agtgtctctt cttaaagaaa ttgcatctac
    32461 acttatagat atacatggtc aacatcaaaa tcaagctttg tttaataaag atactcattt
    32521 aaaatttcta gacctttttg gagaaaatga attggaagag tttaaaattg cttataaaaa
    32581 ggtgtaccat aagtatagtg aagtaaaaaa agctttaaat tgcttgacag aaaataaaga
    32641 tgagatgcaa attcaacgag aaatagattt attaagattt caaataaatg aaattgaagc
    32701 tgcaaatcta aataagaatg aatatgaaga tttattaaaa caaagagaag tttatagaaa
    32761 tagtgaaaag atatataata atctaaattc gagttatagc aagttacata atggtgagta
    32821 taatgttatt gacctaatag gtcttgcatc taaggaactt aatgacatat ctaaatatga
    32881 cagtgtttta agtgaatata gtgatactgt agaaagaatc atgtatgagt tacaagacat
    32941 atctggtgag ataagaaatt acaaagataa tatagacttt gaaccttatg agctagagca
    33001 gattgagctt agaatcgacg agattaataa cttaagaaga aaatatggag acagtataga
    33061 tgatatattt gagtattatg gaaaaattaa agatagatta gatgaaatat taaacagaga
    33121 tgaaagagtt gaacagttaa gaagcaaact gatgaatata gaagaagatt taaaaataaa
    33181 agccagtaaa ttgactaaag caagaataga agtagcaact actttagaaa aaatacttct
    33241 tgatgaatta aaaagcttaa atatgaaaaa tgtaatgttc aaggttaatt ttgaaaaagg
    33301 tccatttact ttagatggag tagatgatat tgaatttatg atatctttta acttaggaga
    33361 agatattaag cctatatata aagttgcatc aggtggtgag atgtccagat ttatgttagc
    33421 gtttaaaacc atattggctg atatagatga tattgataca ttagtgtttg acgagataga
    33481 tactggaata agtgggatag cagcacagat agttggtgag aaattgagtg atatagcaaa
    33541 gaaaaaacag ataatatgta taactcattt acctcaaatt gctgcaaatg cagacactca
    33601 ttattgtata gagaaagata cttcaaataa taggacgttt accaatgtta gtaagttaaa
    33661 tgaatctcaa cgaaaaaatg aaattgccag acttatagca ggtaacaata taacagaaaa
    33721 aactattgaa catgcaagtg aaattattga gttggctaaa aaatgttagt gaaaaacaag
    33781 cattttaata ggtgtaaaga atttttatag aaatgcttta cacctatttt ttataatgaa
    33841 attattttaa aatatctagt gcctatattg aagaaaagta gatttaaaaa tagaagaaaa
    33901 tttgtataaa tttgtaactt tgaaatttct aaatgcttaa gtataatatt atttattaag
    33961 gtagtagtga gggggacata aaatgaataa aaaagatact atgcacttaa tttttacaat
    34021 tgtaattttt attttattat ggtatattgt ggtagcagta agtgagatta ataaaaatat
    34081 tgaaagttgt agtaacaaat tagattattt gtcacaagag attatgagta ctaaatcgga
    34141 tattagtaat actataaatg aagaaatgtc aaaaagttat ataacaaaga gtattgactt
    34201 aaaagtaaaa aaaatcgaga aaaaagagtg tgttattgat gtagatgttc aattaagtaa
    34261 acttggaaaa aatggaaaag tattttttat gtataaagaa gatagtgata aatggaatga
    34321 aactgagatg acaaaacatg gagaattatc atatgcatgt gaaataaaga taaatacagg
    34381 aagtgaatat gactataaag tagttacaag tggagctata tctgaaagtt ctgatgtgca
    34441 aaaattgact aaatctgatt atatgcctga acaacccata tttaattctg ggataagaga
    34501 taatagagaa tactttattg aaatagtaga aaatagtaat cttttcaatc atgagagtga
    34561 aaagtcaaaa aataaagttt atgataatat tagattagaa aaagtagata ttattgtaaa
    34621 tgaaggaaaa aaagacacta tatacaaagc caaattagct gaaggcttag atgatggcaa
    34681 aactaataat tcaaatagaa atcaagtgaa ttatgaagta agttttccta aaagggatat
    34741 tgatggcata atttatatta aagctaaact tacatacaat aatggagtag aatatacaaa
    34801 ggatataact gaagatataa ctatgggatt acaagattta gaaaaataaa tacatattta
    34861 aataaatatt caaatagatt tgttcaataa aatagagtat aaataaaact gtagtagttt
    34921 tacttatact ctattaaaaa attataattt taattccata tatctagccc cttcaattgg
    34981 attataagta taaggttcta tatcataaaa accaaacttt tcgtataatc cttgtgcact
    35041 tttcatgctt ggaagagtat ctaatctcat gtgtgtatat ccaaattttt tagcttcttc
    35101 aatgatttct tccaatagta tttttcctat ttttaatcct ctaaattgat ttcttacata
    35161 taatcttttt aattcacata ttttaccttc aagttttttt aaagcaacac aacctgccaa
    35221 attttcatca acaaatgcca atatcaatga gcctgaaggt tttttatatt ttcctggaag
    35281 tgtttttaat tcattattaa agtcttgaaa acataagtct atatttagag aatttgaata
    35341 ttcagtaaat agaagtttta cgttttcaag gttttctaaa tctttttctt caacaaattg
    35401 taaagttata ttttgagcca ttttatccgc cattatatac acatgtttat ataatgactt
    35461 cacctccttg atatatgtat atttttctta atatcaatta gattttaata ccattacttt
    35521 ttcaccacca tagtcaagct ctccattaaa ctcaaatcca aaatctttat aaagcttaat
    35581 agccatttta ttgtcttcga atatactaag atatagttca tcacagttat attcattttt
    35641 aagatgattt acaagaaaat ctaatgatgc ttttccatat cctttacctt gatattttga
    35701 atcaatcata aatctatcaa gccaaactct atcatcttct aaacgaccat acatagcaaa
    35761 tcctattaaa gaattttcat catatattcc aacaggtacc caagctgtta taaatttaga
    35821 ttcagcaata gaaagtgcat tgctttctat ataatctaac tgacttttat ctacagacaa
    35881 actaactaaa tcatgccaat tatttatgtc tgcaattctt acatttaatt tcaagatatt
    35941 accgcccttc aaactttttg ttacaatcta atactatcat attatgtatt aaaaatcact
    36001 tattaagatt ttaaataaac tttttaaatg aataaattct caggaaaaag gaattataaa
    36061 tacataataa tacagcatag gagtagatgg atatatgaac aaaataaata aaaatttcaa
    36121 aaatatttgg ataatattag gagctgtatc gatttttacc atggttataa tttttaataa
    36181 aaattgtaat gttaataaag agactcaaat agaagctttt acaattaaga atcatgataa
    36241 aaaatttgta tatcctatgg gaaatataat tggagtaaaa gcaaatacag atggtgtatt
    36301 ggtcttagga tatgaggaag aggatgtaga ctatattgga ggcatacaaa tcggtgataa
    36361 tatagtaaaa atagataata aaaggataaa aaatagtcaa gatgtatcag agattttgaa
    36421 taagattaaa aaaagtaaag ttgaagtgac ttttgagaga aaaaataagt ataaaacaga
    36481 agtaatagag actaaaaaag aaaatggcaa gtataaactt ggattatggg taagagataa
    36541 aatatctgga attggaacta tgacttttta cgaccctagt atggaaaagt tcaaagcaat
    36601 cggtcatgcc ataaaagatt cggatactaa tgaactatta aagataaagc aaggatatat
    36661 atataagcca gaacaattaa aaatagtaaa agctagtaat gaaaaagtag gtaaaataaa
    36721 aggagatttt aatgacagca atttaatggg taatttctca aataattcag agctaggtat
    36781 aagtggtaat attacagaaa accataataa agagtttaat gtagcaaata aagaaaagca
    36841 attaatagaa gttggaaggc cgcaagatgt aaaaatagga gatgcagtaa ttctttttga
    36901 ggataaaaac aaaaatataa caagctatga tataaaaata gaaagtatag tatatgataa
    36961 aggaaattat agagatatgg taataaaagt agtagatgac aagttattgg aatacacagg
    37021 aggtatcgta caggggatga gtggagctcc aataatacaa aataataaaa ttattggtgc
    37081 aataactcat gtttttagag ataatccgaa aaaaggatat ggtattttta tagatgaaat
    37141 gataaaattg taggtgaggc attaaaaaat tttattattt tatcaattat ctaggaggaa
    37201 tataattttg gagtgtcgaa tatgctttag agtagataat taggaagcaa ttgtgtaaaa
    37261 agtttagttt tctgtaataa gaagatgttt tttaatgggg ggatttttag tggaaaaaat
    37321 caaaatagtt ttagcagatg acaataagga tttttgtcag gtattaaaag agtatttgtc
    37381 taatgaagat gatatcgata tattaggcat agctaaggat ggaattgaag cattagactt
    37441 agtaaaaaag acacaaccgg acttattaat actagatgta ataatgccac atctagatgg
    37501 attaggtgta attgaaaaat tgaatactat ggatatacct aaaatgccaa aaataatagt
    37561 actatcagca gtaggtcaag ataagataac tcaaagcgca ataaatctag gagcagatta
    37621 ctacatagta aagccatttg attttgttgt gtttataaac agaattagag agctagtttc
    37681 taatagagtt acacaagttg agccaaaacc taggccagtt caagagactc aaatgactag
    37741 aagtgatttt gttaagaatg taggaaatat agagacagaa atcacaaata taatacatga
    37801 aataggagta ccagctcata taaaaggata tttatattta agagaagcaa taaaaatggt
    37861 tattgataat gttgagcttt taggtgcagt aacaaaagag ttatatccaa gtatagctaa
    37921 aaaattcaat acaacaccaa gtagagttga aagagcaata agacatgcaa tagaggttgc
    37981 atggagtaga ggaaaagttg acacaataaa tcaattattt ggatatacag tacacaatac
    38041 taaaggaaaa ccaactaatt cagaatttat agcaatgatt gctgataaat taagactaga
    38101 acatagtatg gttaaataaa caagacataa aaagtaaggc tttttaatta aggcattggc
    38161 tataaatgcg tattacaagc agcgaaacgg tataaccact agggttatac cgtttcgcta
    38221 ttttaataaa tataaaaatt ttctttatta tttgcttact atatcaatat aataatttta
    38281 ttatactatg gatatagtat gtgtctttac aagttgtaaa ctgacagtgg tttatttttt
    38341 aatataaata ttgactttga tgcaggtaaa ctttgtattt ttaagcgtat tatggaatat
    38401 gttaaataaa aaaatgatga aatatagtat tgtaaatgcc aaagatgcaa aacaaactta
    38461 aaacatttat tttattgtta agtaatgcta taatataatg tgattttaat aatgatagtg
    38521 gaggtttaaa tatgagagtc gaggccccta taaaagtaga tcgaaaaacc aaaaaacttg
    38581 ctaaaagagt tgaaagtggg gaaatagcag ttataaatca tatagacata gatgaagttg
    38641 ctgcaaactc tttagtagaa gctaaaataa aacttgtcat aaatgcggct ccttctataa
    38701 gtggtaggta tcccaataaa ggtccaggga tattaactga aaataatata ctaataattg
    38761 ataatgttgg agaagagtta tttgaagaat taaaagaagg agaaactata gaagttgtag
    38821 atggaaagat atatagaaaa ggaaaatttt taggtgcagg agaagtttta gacaagtatg
    38881 aggtttcaca ccaaataaag gctgcctatg agaatttagc tgtagaattg gatagattta
    38941 tagataatac tatagattat gctaaaaaag aaaaaggatt tatacttgga gaagtagaaa
    39001 ttccaaaagt gaaaactaat tatgctaata aacatgtatt gatagttgta agaggtcagg
    39061 actacaaaga agatttaagt actatgcttt cttatataga agaggtaaag ccagtattgg
    39121 ttggtgttga tggaggagca gacgctttaa ttgagtttgg atatactcca gatgtgattg
    39181 taggtgatat ggatagtgta agtgatgaag ctttaaaaaa ggctagtgaa atagtagtac
    39241 atgcttatac agatggaaga gcaccaggtc ttaagagggt tgaggaatta ggcttagatg
    39301 cagttgtatt tcctgcacca ggaactagtg aagatatagc tatgcttata gcctatgagt
    39361 ataaggctga gttaatagta gcagttggta cacattcaaa tatgattgat tttcttgaaa
    39421 aaggaagaaa aggtatggca agtacttttt tagttagact aaaaataggt tcaaagctta
    39481 tagatgctaa aggtgttaat ttgttataca gaagtaaatt aaaaataaag tacatatggg
    39541 cattaatagc aacagcatta tttcctgtgt tggtagttgc atcattgtca ccaggagttc
    39601 aacagttcat acaactaatg caattaaagt ttagagtatt attacagatg taaattttag
    39661 gaggataaat atgcatatta atatgaaata ttatatagtc actatagggg ctatatttat
    39721 agctttgggt ataggcatgt tagtaggatt taatttaaat aataatcaac aattaagtga
    39781 acaacaagca aacataataa atgatttgga tgataagttt aatatcttaa aagaaaaaaa
    39841 tgataaactt gacagtgact tagcaagtgt aaatagagat tatgaagagg cagttaattt
    39901 tataaataaa aatgtagata agattttagc tggttcgctg aatggtaaaa gtataggaat
    39961 aatatctgct aatgaaaatg acgattatac aaaaaatata gaagacataa taaataaatc
    40021 taatggaagc attgcattta atataatatt aaaagaaaac ataacaaatc cagaaaagtt
    40081 aaaggaaatg tcaactaaac taggtacaga tgtaaaaaat gcaaatgatg cagttaatta
    40141 tataattgac actcttaaaa aggaagatgc aagtgacatt ttaacatatc ttcaagaact
    40201 agatgtaata aagtttaatt ttattggaga tacatatcta aaatatgatt ccgttgtaat
    40261 agctggtgga aatgatgcaa aagacagtac aaaacagttt gaaaaaatag aaaaatttgt
    40321 agtttcaaaa cttaaatcag agaataaata tttagtagaa gttcaaaata caggagttaa
    40381 aacttcttat gtagaactat attctaaaaa caaagttgca actatagaca atattgatga
    40441 aggtatagga agtatatctc tagctattct tctacaacaa ggtaatatag ttggtaattt
    40501 tggtagattg gatactgcaa cttcattgtt gccatctatt aaatagaaat aattttaagg
    40561 agagaaaaaa tggattctta tataggtata ataataccag cttataatga agaaagtaga
    40621 ataaaatata cattagaaag catattggac attaagcaaa taaatgaaat aatagttgtg
    40681 gatgatggtt caattgacaa tactgctaag gttgtatctg agctagaaaa tgaaaagatt
    40741 aaatttttta agttagataa aaatagaggt aaaggatatg ctttaaatta tggtatcaaa
    40801 ttagcaatga aaaatgcaga tattataggt tttctggatg gagatttagg aagctctgct
    40861 aaagaagttg aaaaattaat agtacctata ttaaatggtg aggcggatgt tacaatagcc
    40921 aagtttcctc ctgcaaagag aaaaggaggt ttgggatttg taaaaggttt agctaaaaat
    40981 tctgtgtttg agatgactgg tgttgagtta gatgcaactt tgtcaggtca aagaatattt
    41041 aaaagagaag ttttagaaaa gtttgatgaa ataccttttg gatatggagt agaagttgga
    41101 atgaccattg atatattaaa gtatggattt aaaattaaag aagtattagt aaatatgact
    41161 cataatgaaa caggaagaaa tttaaaaggt tttattcata gagggaaaca gtattatcac
    41221 ataaaaaagg ttttaggaca aaaaaagaaa gaatggaggt aatatcttag tgaaagatta
    41281 tatactttat accattttat tattaactgg attatttgga acctatgctg taattccact
    41341 atttaaaaat ttattgataa atggaaatgt acttagacca aactataaaa aagatatgat
    41401 accagtaagt atgggaattg tatttttgcc tatgattgta atcaatggaa taatacttgc
    41461 tttctttact acagaattta aggatttagc gtatatattt atgtttatgt ttggtatgat
    41521 atccatgttt tttgcaggga tactagatga tgtcattgga aatagagatg taagtggttt
    41581 aaaggggcat tttaaaagtt tatttaaagg aagtcttaca acaggaggtt ttaaagcttt
    41641 atttggtgga tttgtagggc ttatagtttc tgttgctata tcaaaagaca taattgatat
    41701 aatagtaaat acactaataa tagctctatc aactaattta atgaatcttt tggatttaag
    41761 accaggaagg gcaataaaag catatttatt cataatggta attatatttt taacattgac
    41821 aggatttgtt caagttcttc cattacttat agtaccaaat gtacttgcat actttaatta
    41881 tgatttaaaa gcaagggcta tgatgggaga tactggttca aacgtccttg gaatatctat
    41941 tggaatgctc atttcttttg ggtatccatt taacattaga ttaggatggt taatatttgt
    42001 agttattatg catatattca ctgagaaata ctcattaact aagataatag aaaaaaataa
    42061 actattaaat tttatagata aattgggaag gtagtattat gataagcaaa agagtgggga
    42121 ttgttgaatc aatagtaagt caaactgaaa cactagatga tataagagta aatataaatg
    42181 gagaaataca aagggcatat aattatccca aaatatctgg aactataaat ataggtgacg
    42241 aagtggtttt aaatacaaca gcagtagaac tgagcttagg tacaggtgga taccattttg
    42301 taataactaa tttaaataat atagaatcta ctttaactga aggtggacat ataatgaaac
    42361 ttagatatac gcctcttcaa ataaaagtgg attctgttga ggaacaagaa agtatttatc
    42421 atgataaatt tgcgaatttt aaaggattag aaggtctttc tgttgtagta gggactcttc
    42481 atagtatgtt aactccattt gcagcttcat ttaaaagaaa taatccaaat aaaaaattag
    42541 tgtatataat gactgatgga gcatctcttc caatttattt aagtaaaaat gtggatactt
    42601 taaaagaaaa aaaattaata gatagtacaa taactatagg aaatgcgttt ggtggagatt
    42661 atgaatgtat aaatatatat actgcattaa ttacagcaaa agaaatacta aaagcggatg
    42721 cagtatttgt cagtatggga cctggaattg ctgggacagg aacaaaatat ggatttacag
    42781 ggattgaaca aggctctatt cttgatgctg taaaaaaact tgaaggaaga gcaattgcaa
    42841 ttccaagaat tagttttgct gacaaacgag aaagacatca aggtatttca catcatagta
    42901 tgactgtatt taaagaaatt gtaaatgtaa atgtagacat accaataact atatatgatg
    42961 atgaaaaatt gaactttata aaagagcaaa tcaatttaaa tggtttagac aaaaagcata
    43021 atataattta tatagataat aataaaacga aagaggattt agactatttt aatctaaaag
    43081 taaagagtat gggaagaaat tatgaacaag atgaggcatt tttcaaagct gcaagcacag
    43141 cagcatatta cttaatggag gtttaagatg gtattagaag aaaaaactat tagtagtgat
    43201 agggtttata caggtaaagt aattacttta aaggttgata ctgttgaaat tccagggcaa
    43261 ggatatcaaa aaagggagtt agtagaagtt ggtggagcag taggaatagt tgcaatcaca
    43321 gatgataata aagttgtgtt agttaagcaa tttagaaaac ctatagaaaa gccaatcttt
    43381 gaaattccag ctggtaaact agaaaaaaat gaaagtccaa aagaatgtgc tgaaagagaa
    43441 ttaaaagaag aaacagggta tagtgcaaaa aatattaagc taatacataa atttttcact
    43501 tcagcaggtt tttctaatga gataatgttt gtatatttag caacaggtct tactccagga
    43561 gagaataatt tagatgcaga tgagttttta gatgtctatg aaattgagct tgaagaagca
    43621 tataacatgg tattaaaaaa tgacgttgaa gatgcaaaaa catctattgg attattatta
    43681 gtaaaagata tgtttaaaaa ttaatattta taaaatgaat aaccctttgt cctaaagcat
    43741 attattttat aaaggacaag ggggaattaa attgagaaga acatatagaa aaaatgattt
    43801 tagagaatta aatagacaaa taattattat aggattgttg tttatgatgt ctattgttgt
    43861 tgggtcttac ataaataaaa tattgccagg ttctagtaac aatatattaa acaatataaa
    43921 tcctgctgta gagtattata atctaaatat aagcataaaa gatacagtga tacaaaattt
    43981 aaaatcagat gcaatcttta tgggctcaat agctctatta agtctatttg tagtaacaat
    44041 tccagcagtg ttggtagctt ttgtattaaa agggatgtca attggttata caataaatag
    44101 ttgtatattg gcattaaaat ttaaaagtat aaaaatgatt ttaataatat tatttaaaaa
    44161 tttgattatt attccatgtg caatcatatt agcattaatt agtttaagtt attttaaaga
    44221 aatggtttat gaatttaaaa agaaaaatag aaaaaatatg caatttttaa ttaagaggta
    44281 tatactaaat attataatta taatagcact atcattgggg ttgcaattaa tattaaatac
    44341 tgcaagtatt ggtattatta agttcctagc taaataaagg agtttataat ggatattata
    44401 gagggatata tagattacat aaaagataaa aaaaaattat cagagaatac agtggcatca
    44461 tactttatgg atataaaaaa atatatggaa tatataaatc aaaaaagcat taaactagta
    44521 gatatagtag aaaatgatat tataagttac ttaataaagt tggaaaaaga taatgtatca
    44581 atagctacaa tagcgaggat gatatcctct ataaaatctt ttcatgatta tttattttta
    44641 aatcatatat gtacaaataa tcctgcaaag gatataaaaa aacctaaaat aaaaaaagaa
    44701 aatataaata tacttacaga agaagaaatt gaaagactat taaattttcc aaagttaaca
    44761 acacctaaat tgattaggga caaggctatt tttgaagtat tatatggtac tggtataaaa
    44821 gtatctgaat tagtagagat gaatatagag gatattgacc tagatattga ttatatatat
    44881 tgtaattcat gttgtaaaaa tcaaagagtg ataccacttt gtgatattac aaaactttac
    44941 ttagaaaagt atctaaaaga agcaagacct aaaatggctg tagaaggtga aaaatcatta
    45001 ttcgtaagtt cattaggtca aaggtttaca cgacaaggtc tttggaaagt cataaaaaaa
    45061 tattctaatc tagccaatat agataaaaat ataaatccta caatgcttag acattcattt
    45121 gctattcatt tgttaaatga aggtgctaac attgctgttg taagtaaaat tttaggaaat
    45181 gttaatttat ctagtttgca agtatattta aatcatatag ataaaaacgt gagaagagaa
    45241 ataaaggaga aacatcctag aaatgatgtt gatgtagaat tacaaaaagc agaagctaaa
    45301 tagactatat agcaaatttt ataatttaga aggagcatat ttatatgagc agagtaattt
    45361 ggattgtaat tgatagtgtt gggataggag cattacctga tgctgaaaaa tttggtgata
    45421 gtaaggatgt aagtaccctt ggaaatatat ttaaggaata tccagatatt cagattccta
    45481 atatgagaaa tttaggtata ggaaacatag atggtataga tttttttgaa agtataaaag
    45541 aaccaatagg ttgttttgga aagtgtaagg aaatgtctca gggaaaagat actactacag
    45601 gtcattggga aatgactggt ataatagttg acaaaccatt taagacattt gaacatggat
    45661 tctctaagga tataatagaa gagtttgaga aaaaaactgg aagaaaagta gtaggaaata
    45721 aaccagcttc aggaactgta ataatagatg agtatggaga gcatcaaata aaaactggag
    45781 atgtaatagt atatacatca gctgatagtg tatttcagat tgctgctaat gaagaagtaa
    45841 ttcccctaga agaactctat aatatgtgca aaatcgctag ggaaataatg atgggggata
    45901 atgcagtagc tagagtaatt gctagaccat tcataggtaa gaaaaaaggt gagtttgtga
    45961 ggacttcaaa tagaagagat tattcactag acccatttga accaacagtt cttgataata
    46021 taaaagaatc aggacttgat gttttagcag ttggaaaaat agaagatatt tttaatggaa
    46081 aaggaattac agatgctatc cacactaaaa gcaatatgga tggagtggat gaaactttaa
    46141 attatatgaa acaagataat aaaggtctaa tatattcaaa tctcgttgat tttgattcta
    46201 aatatggtca taggagagac ccagaagggt ataagaaagc tcttgaagag tttgatagta
    46261 gacttcctga aataatggct aatatgagag aagatgatat tttaataatc aatgcagacc
    46321 atggaaatga cccaacatat aaaggtacag accatacaag agaatatata cctgttatga
    46381 tttatggcaa taagattaaa aaaggtttta atttaggagt aaaagatact tttgctgata
    46441 ttggagcaac tgttgcagat atactaaatg taaagttgcc taaacatggg tcaagcttta
    46501 aagaagattt attttaaact ataaaatgat aaaaacttag cttaatagga tggagagatt
    46561 ggaatgtttg aaaagatagc acaaagtagt aaatttataa attctaaaag taatataaaa
    46621 cctaagattg gtttgatatt aggttctgga ctaggtgatt tagcaaatga tatagaagac
    46681 cctgtcacaa ttaaatatag tgaaattcca aacttccctg tatcaacagt agcaggacat
    46741 gctggacaac ttgtaattgg aaaacttgaa ggaaaagaag ttattgctat gcaaggtaga
    46801 tttcattact atgaaggata ttctcagaaa gaagctactt ttccagtaag ggttatgaaa
    46861 gaattaggag tcgagattct tatagtaact aacgctgctg gaggcgtaaa taaagaattt
    46921 aaagcaggag atttgatgct tataagagac catataaact ttagtggaag taatccatta
    46981 gtaggaaaaa atgatgacag atttggtgct agatttccag atatgtcaga tgcatattct
    47041 tctaaatatg tagatgtagt taaaaactgc gctaaagaat gtaatataga tgtcaaagaa
    47101 ggcgtttata tgttttttag tggtccaaac tatgaaacac cagcagaagt gagaatggca
    47161 caaattttgg gagctgatgc tgtaggtatg tctactgttc cagaagtcat agtagcttct
    47221 cactctaata taggagttat tggtatatct tgtataacaa atatggctgc tggcatctta
    47281 aatcaaccac taagtcatga ggaagtaata gaaactactc aaaaagttaa atcagaattt
    47341 atgaatttag ttaaaacaac ggttaaaaat ttataagtta aagcttatta tagatttttt
    47401 gaggaagttt ataataaaat agttaatgtt taatttgaaa agaattaata aaaagctgtc
    47461 ttgatataga aagttatttc taataaagga cagttttttt ataatactac aacatttgaa
    47521 agtgttttaa agtttaatat ttatttaaat tattataggt tagaatggag atttcaatta
    47581 tgagaatgta tgatattata aaaaaaaaga gagataacct agagcttagt aaaaaagaga
    47641 taaactactt tatagaaaac tattcaaagg gagatatacc tgactatcaa gcttctgcac
    47701 tattaatgtc gatttattta aataagatga ataaacagga aactgtacat cttacagaag
    47761 caatgatgaa ctctggagac atgataaacc tatctgagat agatggaata aaggtagata
    47821 agcatagtac tggtggtgtt ggagataaga ctaccatagc tcttatacct ttggttgctt
    47881 catgtggagc accagttgca aagatgtcag gtagaggtct tggtcatact ggtggtacta
    47941 ttgataaatt ggagtctata ccaggttttt cgacagaaat ggagataagt aaatttattg
    48001 attctgtaaa taagtctaaa atagcagttt gtggtcaaag tgcaaaggta gcaattgcag
    48061 ataagaaaat atatgctctt agagatgtta cagcaactgt tgataatata tcattgatat
    48121 catcaagtgt aatgtctaaa aagttagctt caggagctga tgcaattgtg ctagatgtaa
    48181 aaactggtga tggagcattt atgaagacat tagaagagtc ttttgagtta gctaaatcta
    48241 tggttgatat aggtactggt atgaatagag atacaatagg gattgtaaca gatatggatg
    48301 aacctttagg ttttgcagtg ggaaactctt tagaagttat agaggcaatt gagacattaa
    48361 aaggaaatgg acctaaagac cttgttgagc tatgcgaagt attaggagca tatatgttgg
    48421 ttttatcaaa ggtagcacat gattttgaag atggtgttga aaaaataaga gaatcaataa
    48481 aatctggtac tgctatacaa aaactcaaag agtttataga gaatcaaggt ggtaataaag
    48541 atgtagtaga tgattattct ttattcaaaa aatcaaagta taaatatcca gttaaatcaa
    48601 ctaaatcagg ttatataagc aaaattaaag ctgaagatgt tggtgtatcg gctatgattc
    48661 ttggagcagg tagagaaact aaagatgata ttttagattt atcagcaggg ataattttag
    48721 aaaagaaagt aggagattat gtaaatgaag gtgaagtttt agcttacatg tattataatg
    48781 atgaacaaaa gctttcattg gcaaagggaa gatttattga ttcctactct attgtagatt
    48841 caaaaataga aaaaaataaa ttaatatatg gtattgtaac taaagaagag attaaaaagt
    48901 tttagaaaat tttagaaaag tgattataaa aggcctaaca tgtcaaaaat aatgttaggt
    48961 tttttatttt ttgataaact ataaattata ccaaaaatat atattttaaa acatttttta
    49021 caatataaat aagtttattc tacaagatga ttgaaaatta aagtatctag ttttataata
    49081 gaaaatggat aaatatacat aaggagtaga ttatattatg aactttaaag aaaatagatt
    49141 gaaaatagca gtattaataa ttgttatttt gattatctta gcaggtatat ttgtgtttat
    49201 acaaataggt ccctatgata aaaataacaa aaaagatgta attatcgacg ttccaagtgg
    49261 agcatctgtt ggaaaaatat ctgacatatt atatgaaaat aaattaataa agaatgaact
    49321 attatttaaa cttttggtaa aggttagcaa taaagctccg tctataaaat caggaacata
    49381 cttacttaat caatcgtatt caaacaatga tataataagt cttttagttt caggtaaaat
    49441 atatcaggat ggtataaaag tgactattcc agagggagct acatctaaag aaattatagc
    49501 aatgttagta agtaaaaatt taggagataa agctactttt gaaaatttaa taaaaaaacc
    49561 tcaagaattt tatgataaat ttctatattt gaaggaagat ggaataacct ctttagaagg
    49621 gttcttgtat ccagaaactt attattttaa ttcaaagaaa caatcagaag aagatatttt
    49681 aagcgaaatg ttaaaagtat ttgacagtaa atacacggac aagtttaaaa agaaacaaaa
    49741 agaattaaat atgactcttc aagaagtaat ggaaatggca tcaataattg agaaagaagc
    49801 tgttcttgac aaagacagac ctataatagc aagtgtgttt tataatagac ttaaggtagg
    49861 tatgcctctt caatcagatg ctacaataca atatatattt gaagagagaa agaaaattgt
    49921 aacatatgat gatttaaaaa ttgattcacc atataatagt tataaaaata agggattacc
    49981 tcctacacca atatcaaatc ctggtataaa atctattgaa gcagcattat atccagagaa
    50041 aacagactat ttatattttg tagctaagat tgacggtgga aataattata gtacaaacta
    50101 tcaagaccat ttaaaatacg ttaaagaata taaagaagca agggataaac aatcaaaaga
    50161 tacaaaagca acaaataaag aaaatacaaa aaaataaatc atgattataa aaagtctaac
    50221 agggtaagaa taatgttggg cttttttatt gaaaattgtt attcttagat ttataataaa
    50281 gaaagtgtaa atatatataa caatatgaat ttaaaaaata ataaattaat ataaaacttg
    50341 aatatttatt attttaggga gtgtttttat gagtaatata gttaatgatt tggttgaaga
    50401 ttatataaga gtgacattaa aagaaaaaga aggattttta aaagatttag aaatttatgc
    50461 agaggaaaat agtgttccta taatacataa agaagtttca gatttattaa aagtcttgtt
    50521 aaaggttcaa aaaccaaaga gaattttaga agtaggatgt gcaattggtt attcttctat
    50581 tttttttgca actattatag gaaaagatgc agatataata actgttgaga gaaatgaaaa
    50641 aatgatagaa aaagcaaaag aaaacataaa attagctgga tttgacaata acataactat
    50701 actagaagga gatgcagaag aaaaacttag tcaagtccaa ggtgaatttg accttatatt
    50761 tatagatgca gccaaaggtc aatataagct attttttgat ttagttatag ataaattaaa
    50821 agatggggga cttttagtat ctgataatat tttgtataaa ggtatggttg ctcatgatga
    50881 ttttgttgta agaagaaaaa agactatagt taaaagaatg agaaattatt tagattatat
    50941 atgtaattgt gactatttaa gcacatcatt ggtaccaata ggtgatggtg tagcactaag
    51001 ttataaagaa agtaaaagag gtgatgtaga tggaattaaa taaagtagaa cttttagctc
    51061 cagcaggtga tttggagaga ttgaaaatag ctataacata tggagcagat gccgtttata
    51121 taggtggaga aatttttgga atgaggtctg cagcgaagaa ttttagtaag gaagatatgg
    51181 cagagggagt agcttttgca catgaaagag gcaagaaagt attcgtaaca gtaaatataa
    51241 tacctcataa tgaagatttt ctacaattag aagactattt gttggaacta gaagagatag
    51301 gaatagatgc agttataata gctgacccag gagtgcttag tgtcattaaa aaagtaattc
    51361 cgaatatgga aatacattta agtacacaag caaatacaac caattatcta agtgcaaact
    51421 tctggtacga gcatggaata aaaagagttg tagtggcaag agaattatct tttgatgaaa
    51481 tatctgagat aagagcaaaa acacctttag atatggatat agaagcgttt atgcatggtg
    51541 ctatgtgtat ttcttattct ggcagatgtt taataagcaa ctatatgact ggaagagatg
    51601 caaacaaagg ttcttgtgct caatcttgta gatggcaata tcatttggtg gaagaaaaaa
    51661 gacctggtga gtattttcca atatacgaag atgaaagggg tacattcttc tttaactcaa
    51721 aagatttatg tatgatagag tatataccag agttaatcaa atctggtata acaagtttaa
    51781 aaatagaagg tagaatgaag actgcatact atgtagctac tgttgtaaga gcatacagaa
    51841 tggcaataga tgaattttac agagacccag aaaactggaa gttcaatcca atgtggatgg
    51901 aagaactaaa aaaaggtagt catagacatt ttacatcagg tttttacttg aataaaccaa
    51961 caacagaaga ccaaaattat caatcagcat catatgttag aaactatgat tttataggta
    52021 tagttagaga aacagaagat gaagatggtc ttattattgt tgagcaaaga aataaaatgt
    52081 gtgttggtga tgaaatagaa gttatgggac catataaaga aactatgttt actaaaattg
    52141 aagaaatgta caatgaagaa ggagaagcta tagaatcagc tcctcatcct agacaaatag
    52201 tgaagttaaa gctatcagta aaagttggta aagattatat gcttagaaaa gttatagaag
    52261 aaaaggttga agaataataa aaaattgata aaaatagtat tagcccctta gaaattgtca
    52321 ataatgaaat tatctaaggg gtgatttcat gtcaaaaaag aagactccat ttttgaaaaa
    52381 agtgggaaag agaagctggt gtatatttac tataatttta ataatttata gtgttttaat
    52441 ttataggcta gttgatatac aagtattaaa aggtgataaa tacaaacaaa gtgttgaatc
    52501 acaaagcgtt gagaaagttg agttaaatag tggaagagga ataatctatg atagaaacaa
    52561 caagaagctt acagatacaa gtaaatctca ggtattaatt gttgaaaaag aaaaattaaa
    52621 taataactat aaaattttgg agcttattaa gaaagctact aagatgaatg atttagatat
    52681 atataaagct gtacaagagc aattgacacg ccctataata cagattcaga ctaaaaatat
    52741 cgataagagt atgaaaaaag aacttgagaa aaatggaata atggtagaag aaaaaactat
    52801 gaggtacgca aaagatggtt tattatcaca tacaatagga tatataaaag aagatgataa
    52861 atcaggtcaa tcaggaattg aaaagagtat ggatagtgta cttagaaatt caaatgaaaa
    52921 gtatataagt gcttttaaag ctggagatgc aggaaatgaa aaatctctaa acatactaaa
    52981 gggaagtgta aaaactgttg ataataagga taaagataga catctaaaaa ctactattga
    53041 ttataacatt caaaaaaaat tagaacaaat actaaataaa gaagaaaatc ctacagcagc
    53101 aataatctcg gaagcatcta caggtgaaat tcttgcaatg tgctctagac ctaattttga
    53161 ccaaaatgac atatcaaaaa gtttaaaagg aaaaaatggg gaatttgaaa atagagttat
    53221 aaaagcgaca tatccaccag ggtctgtatt taagatggta gttttatttt ctgcacttga
    53281 aaatggtgtt atagatgaga actatactta caactgtaca ggtaagacta aagttgggaa
    53341 tactaatgaa atactgcgat gtaataaaag agatggtcat ggtttccaaa acttaagaca
    53401 agctttttct aattcatgca atcctgcatt tttagatata gctatgaaac ttggaaaaga
    53461 aaagatactc aaatctgctg aaaaattaca tttatttgaa aaggttgata ttggtcttga
    53521 tgaagaaaaa attagagaag ctccaaaaaa catatcaata agaaatttag ctataggtca
    53581 agaaaatata gaatttacac cacttcaaat aaatcaaatg actcaaataa tagctaataa
    53641 tggaacattc aaaccactat acttatataa aagtcttgtg gacaataata tgaatacaat
    53701 aaaaacttat aagtcttcaa aaaaagaaga acttatttct ccttatgtgt gtacacaagt
    53761 taaagaatat atgaagtctg tatctaggat tggtacagct aaagacctaa aagatattga
    53821 aggtggttgt ggagttaaaa ctggtacagc tcaaagtagt ttaaataaaa aagctataga
    53881 ccacggatgg ataacaggat tttatcctga agaaagacct aagtatgtaa taactgtgtt
    53941 agtagaagga actcaaaaag gaaataaatc tgcaacacct atatttaagg aaatatgtga
    54001 aagtataaaa taaattatgt actataaaat ttaaggatat atcgtaactt tgattttaga
    54061 tagaaaactt tgtctaatga tgtacgatat atccttttaa aatatagtat taactttctt
    54121 gaacaagctc tttttctagt tttttaagag cttttttttc gattctagat acatatgacc
    54181 ttgatatatc gagtttttgt gcaatttctc tttgtgtctt atagccataa ggagttaacc
    54241 catatcttaa ctggacaatt tctctttctc taggagtaag aattttgttt agttgttcgt
    54301 aaagttttcc aacttgaact tttagttcaa cttcatcaag aacatagtta gcctctgtac
    54361 caagtatatc taaaagactt atttcattac cttctttatc tgttccgata gggtcttgta
    54421 gagatacttg agttttattt tttttgttag atctaatatt cattaaaatc tcattttcaa
    54481 tacatctaga agcatatgtt gctaatcgag ttcctttatc tatgttataa gtttctatag
    54541 ctttaattaa tcctattgta cctatggata ttaaatcatc ctgatcttcc gttgaattat
    54601 tatatttctt tgctatataa gctactaatc tcatgtttct ttcaattaat gtatcttttg
    54661 cacttttatc gttttctatt ttaaatttag ttaaatactc aatctcttct tcaggggtta
    54721 agggtttttc aaaagattta agagctgcca ataggtcacc cccttttcaa cggtcacttt
    54781 aaattatatg aaggagactt atccaagttg actgttatct aataaaataa tatgcatatt
    54841 tttagaattt aaaacatata tttgcaaact tttgaatgga atatgacata aagagtggga
    54901 atttaaattt aagtttttgt tgtatatata tgaaacaagg cgaagaaatt attgatttat
    54961 aaagtataaa ttggaattga ggtgtcaata aatataaaag gaaatagtat ttatatatgt
    55021 tttggtgttg aagtaaaaga ttaatgcaaa gtattagagt ctataaaaat atgaatatag
    55081 gaggtgtaat tttacaatat ttaaaaaagt aaatgtaatt aattgtttgt
//
LOCUS       DAEMIK010000005        53679 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEMIK010000005 DAEMIK010000000
VERSION     DAEMIK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.005"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..618
                     /locus_tag="KPK87_000251"
     CDS             <1..618
                     /locus_tag="KPK87_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2536452.1"
                     /translation="YFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVKLD
                     LDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDIAL
                     KNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNLTV
                     SIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            645..2531
                     /locus_tag="KPK87_000252"
     CDS             645..2531
                     /locus_tag="KPK87_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2536453.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            2539..4539
                     /locus_tag="KPK87_000253"
     CDS             2539..4539
                     /locus_tag="KPK87_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2536454.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            4801..6060
                     /locus_tag="KPK87_000254"
     CDS             4801..6060
                     /locus_tag="KPK87_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2536455.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            6121..7908
                     /gene="iorA"
                     /locus_tag="KPK87_000255"
     CDS             6121..7908
                     /gene="iorA"
                     /locus_tag="KPK87_000255"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG2536456.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            7910..8485
                     /locus_tag="KPK87_000256"
     CDS             7910..8485
                     /locus_tag="KPK87_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG2536457.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            8538..9608
                     /gene="buk"
                     /locus_tag="KPK87_000257"
     CDS             8538..9608
                     /gene="buk"
                     /locus_tag="KPK87_000257"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2536458.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            9826..10527
                     /locus_tag="KPK87_000258"
     CDS             9826..10527
                     /locus_tag="KPK87_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536459.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            10587..11093
                     /locus_tag="KPK87_000259"
     CDS             10587..11093
                     /locus_tag="KPK87_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG2536460.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            11268..12290
                     /locus_tag="KPK87_000260"
     CDS             11268..12290
                     /locus_tag="KPK87_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2536461.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIIQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(12437..12745)
                     /locus_tag="KPK87_000261"
     CDS             complement(12437..12745)
                     /locus_tag="KPK87_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG2536462.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(12805..13233)
                     /locus_tag="KPK87_000262"
     CDS             complement(12805..13233)
                     /locus_tag="KPK87_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536463.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(13460..14917)
                     /locus_tag="KPK87_000263"
     CDS             complement(13460..14917)
                     /locus_tag="KPK87_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG2536464.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            15335..16249
                     /gene="nadA"
                     /locus_tag="KPK87_000264"
     CDS             15335..16249
                     /gene="nadA"
                     /locus_tag="KPK87_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG2536465.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKTLENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            16262..17563
                     /locus_tag="KPK87_000265"
     CDS             16262..17563
                     /locus_tag="KPK87_000265"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG2536466.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            17544..18380
                     /gene="nadC"
                     /locus_tag="KPK87_000266"
     CDS             17544..18380
                     /gene="nadC"
                     /locus_tag="KPK87_000266"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG2536467.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            18636..18968
                     /locus_tag="KPK87_000267"
     CDS             18636..18968
                     /locus_tag="KPK87_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG2536468.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            19130..19315
                     /locus_tag="KPK87_000268"
     CDS             19130..19315
                     /locus_tag="KPK87_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536469.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            19342..20427
                     /locus_tag="KPK87_000269"
     CDS             19342..20427
                     /locus_tag="KPK87_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG2536470.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            20420..20878
                     /locus_tag="KPK87_000270"
     CDS             20420..20878
                     /locus_tag="KPK87_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536471.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            20913..22004
                     /locus_tag="KPK87_000271"
     CDS             20913..22004
                     /locus_tag="KPK87_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2536472.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            22009..22233
                     /locus_tag="KPK87_000272"
     CDS             22009..22233
                     /locus_tag="KPK87_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536473.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            22243..22806
                     /locus_tag="KPK87_000273"
     CDS             22243..22806
                     /locus_tag="KPK87_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536474.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            22767..23396
                     /locus_tag="KPK87_000274"
     CDS             22767..23396
                     /locus_tag="KPK87_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2536475.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            23436..23573
                     /locus_tag="KPK87_000275"
     CDS             23436..23573
                     /locus_tag="KPK87_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2536476.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            23573..24325
                     /locus_tag="KPK87_000276"
     CDS             23573..24325
                     /locus_tag="KPK87_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536477.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            24338..24823
                     /locus_tag="KPK87_000277"
     CDS             24338..24823
                     /locus_tag="KPK87_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536478.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            24830..24979
                     /locus_tag="KPK87_000278"
     CDS             24830..24979
                     /locus_tag="KPK87_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536479.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(25115..25936)
                     /gene="yidA"
                     /locus_tag="KPK87_000279"
     CDS             complement(25115..25936)
                     /gene="yidA"
                     /locus_tag="KPK87_000279"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG2536480.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            26429..28123
                     /locus_tag="KPK87_000280"
     CDS             26429..28123
                     /locus_tag="KPK87_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG2536481.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETSEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      28455..28565
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            28669..29040
                     /locus_tag="KPK87_000281"
     CDS             28669..29040
                     /locus_tag="KPK87_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG2536482.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            29136..30083
                     /gene="trxB"
                     /locus_tag="KPK87_000282"
     CDS             29136..30083
                     /gene="trxB"
                     /locus_tag="KPK87_000282"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG2536483.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            30168..30485
                     /locus_tag="KPK87_000283"
     CDS             30168..30485
                     /locus_tag="KPK87_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG2536484.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            30754..32040
                     /locus_tag="KPK87_000284"
     CDS             30754..32040
                     /locus_tag="KPK87_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG2536485.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            32152..32625
                     /locus_tag="KPK87_000285"
     CDS             32152..32625
                     /locus_tag="KPK87_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:32284..32286,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG2536486.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            32657..33967
                     /gene="grdB"
                     /locus_tag="KPK87_000286"
     CDS             32657..33967
                     /gene="grdB"
                     /locus_tag="KPK87_000286"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:33704..33706,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG2536487.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            34223..35755
                     /locus_tag="KPK87_000287"
     CDS             34223..35755
                     /locus_tag="KPK87_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG2536488.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            35766..36911
                     /locus_tag="KPK87_000288"
     CDS             35766..36911
                     /locus_tag="KPK87_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG2536489.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            37221..38300
                     /locus_tag="KPK87_000289"
     CDS             37221..38300
                     /locus_tag="KPK87_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2536490.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            38413..39012
                     /locus_tag="KPK87_000290"
     CDS             38413..39012
                     /locus_tag="KPK87_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536491.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(39199..40071)
                     /locus_tag="KPK87_000291"
     CDS             complement(39199..40071)
                     /locus_tag="KPK87_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2536492.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            40488..41891
                     /locus_tag="KPK87_000292"
     CDS             40488..41891
                     /locus_tag="KPK87_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536493.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            41918..43462
                     /locus_tag="KPK87_000293"
     CDS             41918..43462
                     /locus_tag="KPK87_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG2536494.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            43493..44884
                     /locus_tag="KPK87_000294"
     CDS             43493..44884
                     /locus_tag="KPK87_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG2536495.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEVVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            45077..46573
                     /gene="abfD"
                     /locus_tag="KPK87_000295"
     CDS             45077..46573
                     /gene="abfD"
                     /locus_tag="KPK87_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG2536496.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            46708..46983
                     /locus_tag="KPK87_000296"
     CDS             46708..46983
                     /locus_tag="KPK87_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536497.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            47007..48314
                     /locus_tag="KPK87_000297"
     CDS             47007..48314
                     /locus_tag="KPK87_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG2536498.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            48334..49452
                     /locus_tag="KPK87_000298"
     CDS             48334..49452
                     /locus_tag="KPK87_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG2536499.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            49739..50659
                     /locus_tag="KPK87_000299"
     CDS             49739..50659
                     /locus_tag="KPK87_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG2536500.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTVS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTSEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGNKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            50774..51931
                     /locus_tag="KPK87_000300"
     CDS             50774..51931
                     /locus_tag="KPK87_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG2536501.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(52016..53515)
                     /locus_tag="KPK87_000301"
     CDS             complement(52016..53515)
                     /locus_tag="KPK87_000301"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG2536502.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT        20945 a         5910 c         9862 g        16962 t
ORIGIN      
        1 tactttaaaa tgtattatga gttaaaaatg atgtatataa aagctcgaaa tagaaattat
       61 atagattcct taaattatag acatgagtat atagaaaaag aaataaataa catgagaaaa
      121 gtaaaacttg atttagataa aaaaaagtat gttgaccatt tgacaagtgc atataataga
      181 acttttttga acgatttttt agaaaaacaa gaagttagtg aatattgcac agcatttatg
      241 atagatgtag attactttaa agaatacaat gatacttatg gacactataa tggagatatt
      301 gctcttaaaa atataactat aataataaaa aaatatctaa aaaaagacat gcttatgata
      361 aggtatggtg gagaagaatt tctgatatta tctgtatgta aagattataa aaaaagtaaa
      421 atatttggaa gaaaattatg taggattgta aagaaatttt taaacaataa tttgacagtt
      481 agtattggca ttgatacatg taaaaatagt agtatagata taaaagaaat aattgaaaat
      541 gctgataaag ctttatataa agcaaaggaa agtggtagaa atagatgttt gcattatcat
      601 gattttaaga atttttaact gttgaaatag gattggtgat ttacttgaaa aggagtttaa
      661 tagaagattt aaagagaaat ataaacaaga tagaatacga tatgttgact aaattctatc
      721 taaatttgac agatttaaac tacatcttgg attatcacaa agacctttta aatacaaaat
      781 tagaagataa tagtagagtc tatactttat atttaattag tcttataaac tatataaaaa
      841 aagatagaga agcatcatac aactacttga tagagtcatt aaagtatatt aaaaaattgt
      901 cattaaaaga tgattcagat ataatatcta aaatttatat ttatcttact ttatcagcta
      961 tttctataag aaaatatgat aaggtaaata atttttattt ctccgcaaaa aaaattataa
     1021 gacaaaatca tttaaatgag ttgttggtat tgttaaatat gagtttatgt gtggaaataa
     1081 gtgctttata tcagaccaaa acggatgtaa tagctttagt agaagaagtg tcttttttta
     1141 aaactttaaa aaataaagct cttgtaagta gggcaaatta tatatttggg agagtttatc
     1201 tatatttatt taataacttt ataaaaagta tggaatactt aattgaatgt ctaaaattgg
     1261 ctcaagaaaa taatttatac tctatagagg ctttttgtaa atgtataata agtttgtgtt
     1321 atttagagag tggaaataat gtagaagcta taaaatacat aaataatgta ttagatgttg
     1381 cacacagtaa taactcaata tcagtaactg aaagagtgtc tataaaaata gaccttatat
     1441 gggcatattt aaaagaagat atgaataaag aagcagaagt tattttatta aagactataa
     1501 aattaatgaa agttgttgaa ggtgtatata aggattattt atattcattt atatttttat
     1561 attcagctga aattgagtta aagaaagata attgtagttt tgaaaaggta aacacctatt
     1621 taattttatc aaaaaatata tataaaagtt ttggagatgt ttttatatat aattctaaac
     1681 tagattacat atataagttg tatggagatt tatatataaa atttggtaat atagatgaag
     1741 gtataagttg ttatataaaa ggtttaaatt tagttaaaaa gagttcattg aatcttagaa
     1801 aaatatctat attttatggg ctaatagcaa atggatatga gctaaaaaaa gattttaaac
     1861 tggcaataga atattataaa agtatggatt ggtattctga taggtgggaa aaaaataatt
     1921 cctataaaac atctcaagca attcacaaac aatatgaact cagacaaaaa caagagagtt
     1981 taaggttact agtggatgat aataagaaat tagaaaatga tattttaaaa gatggactta
     2041 ctaaactata taatagaaga tatctggaac agcagttgga tttatttaat ggaaccagag
     2101 acaaaaatat ggtcgcaata ctggtcgata tagattgttt taaaagttat aatgataatt
     2161 atggtcatct agcaggagat aaagttatag ttaaggtaac tgatattata agaagtgtat
     2221 ttatagatat taccgaacac ataatcagat atgggggaga agaaatttta atattagtag
     2281 attctataga atcaatcggt gttgaagaga ttgataatat agaattatat attaaaagag
     2341 ttttcaaact tcttgaattt gagggtattg aacatatata ttctaaaata aaaaactata
     2401 taactataag tgctggagta agtataaaaa ggtgtcgtag tagaaaagat gttgaaatat
     2461 taattgaaga tgcagataag aacctatata aatcaaaaaa agcaggtaga aatcaataca
     2521 ttataattta aaattgaggt gattttgttg cgaaaaaaaa taggaattat agcctctgat
     2581 attgaattaa aagaaagaat agaagaatta tatagagaag atgtagaaaa tggaactata
     2641 ataatcgaca tacttaacct agacttaatg gaaaatcaag gtcgtatact tgttgaaaaa
     2701 ggtgcccaag ctataattgg tagaggtgga ggttatagct tagtaataga tacagtaaat
     2761 gtacctgtta taccaatgaa tatgaagtca actgaccttt taagagcaat agaaatagca
     2821 aagaaatatt ctaaaaaagt agtattgatt cttggagaca atgaagtttc ttttgactat
     2881 gttggctgga gaaatgtcat aagtactgaa ataactgaag aatggtttga atctaagtat
     2941 gagataagaa gtaaagttgt aaaatatata gaccaaaaag atgaagttgt aatcgtagga
     3001 ggaggtttgg catgttcttt tgcaaggcaa tatggaattg atagtgtatt tgcaactgct
     3061 agtgatgaat ctataagaga agctgtagaa tattgtaaaa aattattaga tacattaggt
     3121 gaagaaaagt ttaataatga agtgttacga aatatacttg atggtataaa agatggagta
     3181 attgctatag atagtaatgg cagtataatt ttgtacaatg aaagtgcaaa aaacatgctt
     3241 aaagtagaga gaaaatgtgc acttaataaa tacatcttag atgtatttcc taaaatggag
     3301 tggatgttag attgtttaca tgaaaaagag gacgtagaag atagaaagat aagaaatata
     3361 aataatctaa ttgttaatac taggacaaca ttgataaaag ttgataatag tacttatgga
     3421 gttttaggaa taatacaaga tataaccaag ctacaaaact tagaaagaaa aataagattt
     3481 gacttgaatc aaaaaggtct ctatgctaga tatacttttg atgatttttt atttaaagat
     3541 aaattaacta aagaatttat tgaagaagct aaaaaaattg gaaaaagtga ttatacaact
     3601 ttactttatg gagaaagtgg gtctggtaaa gaaattatag ctcatagtat acacaatata
     3661 agtaaaagaa aagataggcc ttttgttgct ataaattgtg ctactatagc agaaaattta
     3721 cttgaaagtg aactttttgg ctatgaagaa ggtgcattta ctggtgctag aaagggtgga
     3781 aaaagagggc tatttgaact tgctcatgga gggactcttt ttttagatga aataaatagt
     3841 ctatccttta atattcagac aaaactatta agagtaatag aggaacgtca aataatgaga
     3901 attggttctg attatataat acctctggat ataaggatta tagcagcaac gaacgaatct
     3961 ttaaccgaaa aaatagttat gggtacattt agagcagact tattttatag attgagtagt
     4021 ttagagataa atatacctcc tttaagagac agaagagaag atataatacc tttgtttaac
     4081 aattttgtaa atgaagtatt aaaggatgat ggtctcaatg gaataaacag tatagatgaa
     4141 aattttgttt tgactaaaga tgaaatggat aagctatata attatagctg gcctggaaat
     4201 gtaagagagt taaagacaat agctcaaaaa tatgttgtga caggtaaaat taaattgaga
     4261 caagatagag attttaaaac taaaaaatta cttcctaact ctgaagtaga caaatttaat
     4321 tctgaaacga cagcaagtgt agaagtccaa gatgaatcta taaatataag taagataaat
     4381 gatggtaaga taagtataga tataaaagaa gtaaataagt atgttgaaga aaaaataata
     4441 agtatgcttt ttgctcaggg actttctaaa aatgaagttg cacaagttct tggaataagc
     4501 aggacatctt tatggaagaa atacaataaa aatatttaaa tataattaca tataaattat
     4561 tttgaagtaa tgttaatttt ttaaaacaag tgttaagaaa aattaacatt acttttttgc
     4621 aaatttttat ttttttagac aggcatatat atttataaga aaaatagtta aaacacttaa
     4681 tttaataatg aaaaaaataa taattctaaa caaaatcaat tttacataaa gtgggattta
     4741 ttggcatagt aattgcttaa tatataatgt aagagtaaat acaattttag gggtgatggt
     4801 atgagtcaaa gtatggttgg taagcatgca atgtggccaa aagaaaatga tgttatattt
     4861 agcatatctg gcagagctca agcagcagag aaggcttttg gtatggataa tgtaataaat
     4921 gcaacaatag gcgctctaat ggatgattca ggaaaattga taacaatgaa aactgtatat
     4981 gaagaatata aagcattaga taactgtgag ataggtgcat atgcagctct tgaaggtcaa
     5041 ccagattact tagaagcagt taaaaaagta tttttcagag attacttacc agaaggacat
     5101 ataagagtat tagcttcacc aggaggaagt ggtgcaataa aattagctgt atggaactat
     5161 actaatgaag gagatgaggt attaacatct gattggttct ggagtccata tgtaagtata
     5221 gctgaagagg ctaatagaaa agtagttaat taccaacttt ttgatgaaaa tagaaggttt
     5281 aattttgagt cgtttaaaga aaaatttgta aatatagcag aaaaacaagg tagagtcttt
     5341 acaattataa acacaccagc gcataatcca actggatata gtgttgcaga tgatgagtgg
     5401 gataaaatat tagacttatc aaaagaagtc gcaaaagata aagacaaaaa gataatattt
     5461 ttcgtagatt cagcatatat tgactttgca ggtgatgatg atgtatgtag aaagtttttt
     5521 aagaaatttt ctaatttgcc tgaaaacgtt ctcgtattgg ttggattcag tatgtcaaaa
     5581 ggatttacag cttatggtat gagaatgggg gctataattt gtatatcttc aagtgaagat
     5641 gtagctgaag agttccatta ctcatgtgtt cattcatgta gagcaaattg gtcaaattgc
     5701 aatagaagtg ccatggcagt attatctaac attgtaaatg accctaaaaa attcaaagaa
     5761 tatgaggatg aaaaagaaat atacaaaaac atgcttacta gaagagcaga tgtatttgta
     5821 aaagaagctg aaagagtagg attagaaatt cttccatata tagcaggttt ctttgtaagt
     5881 ataccatgtg ataatccaaa agaagtatgt gaagaattga ctaaacataa tttatttgca
     5941 gttccattaa aaatgggctt aagatttgca gtttgtgcag tttctgaaga taaatgtaag
     6001 aaagcaccaa gtattataaa agaagcactt gaaagtttag aagtaaaaat aaataattaa
     6061 tttcattgaa caaattgggt ggactatgaa tttaataatt gtagatggag ggaattttat
     6121 atgaaacaat tgatgacagg taatgaagca attgcacgtg gagcctatga ggctggtgtt
     6181 aaatatgcat ctgcatatcc agggactcca agtacagaaa tattagaaaa tatagcaaca
     6241 tataaagatg caatagttgc agagtgggct ccaaacgaaa aggtagcact tgaggctgct
     6301 atagggggtt caattgcagg agcaagaaca atggcttcta tgaaacatgt tggtttgaat
     6361 gttgcagctg atccaatatt tacttatgca tatactgggg taaatggtgg tatggtactt
     6421 attacagcag atgagccggg aatgcattca tctcaaaatg agcaagacaa taggatgtat
     6481 gcaaaatttg caaagatacc tttatttgaa ccatcaacta gtcaagaagc taaagatatg
     6541 ataaaagaag cttttgaagt tagtgaaaaa tacgatacac cagttttata tagagttaca
     6601 acaagacttt gtcattcaaa aggattagtg gagtgctatg atagagaaga agttgaaatt
     6661 aaagagtatg ttaaaaatgc taagaaaatg gtaactgttc cagcaaatgc tcaaataaga
     6721 aggggcgttg ttgaagaaag aatggaaatc cttaaaaaat tctcaaatga aactgattta
     6781 aactactatg aaataaatga tacaaaaata ggtgttatag cctctggtat gtgttgtaat
     6841 tttgccaaag aagtttttgg aaaaaatgca tcatatatga aactaggatt tacaaatcca
     6901 ttaccttatg aaaaaataaa agaatttgca gaaaaagttg ataaaatata tgttatagaa
     6961 gaaaatgacc catttataga agaacaaata aaagcatatg gaatagattg tattggaaaa
     7021 gatgtaatac caccatatgg agaaatgaca ccagatgttt taagaaaagc tatatttgga
     7081 aaaacaaatg atactataga atacaaatct gaattagtaa caccaagacc accaagtttc
     7141 tgtgcaggat gtccacatag aggattcttc tatgaacttg gaaagagaaa aaatttaata
     7201 gttggtggag atataggttg ttatactctt ggatttgctc ctccatataa tggaatagac
     7261 tatgttgttt gtatgggttc tgcttttgga actgcacatg gagctcaaaa agttcttaat
     7321 atgaaggatg ataatgaaaa aagattggta ggtgtacttg gagactcaac attcttccat
     7381 acaggaataa atggactttt agatgttgtt tacaatagag gtaattctat ttcggttata
     7441 ttagataata gaataactgg aatgacaggt caccaagaaa acccaggttc aggatatact
     7501 ttacaaggtg ctaaaactaa agaagttgat atagaaggat tagttaaagc ttgtggaata
     7561 gaacatgtaa gagttataaa ccctaataat ttaaaagaag taaatgaagc actagattgg
     7621 gctttagcta tagaagatga accatcagta ataataacaa gatggccatg tgtacttaaa
     7681 aaattctcta aagaagatat agaagaattt aataatccat ttaagactaa gtgtaaagtt
     7741 gaccatgata aatgtatagg atgtaaatta tgtttgaaaa ctggatgtcc agctctatca
     7801 tttgataaag agaataagtt atcaaatata gatagaaatc agtgtgtagg atgtggagta
     7861 tgtgcacaag tttgtccaaa acaagcaata gttaaggagg aaaaataata tgactaagag
     7921 tatacttttg gtaggtgtag gaggtcaagg aactatactt gctagtaaat tattaactat
     7981 gggattgatg gaagctggat atgatgtaaa gatgagtgaa atacatggaa tgagtcaaag
     8041 aggaggttca gtttcctcac aagttagata tggagattgt gttcattctc cagttataga
     8101 aattggtgga gcagatatat tagtatcttt tgaaaaaatg gaagctttaa gatggttcaa
     8161 ttatttaaaa ccagaaggaa aagctttagt aaataatcat aggatagatt ctatgactgt
     8221 tttaatcgga ggagctgaat atcaagaaag tgatatagat gcagagttaa gtagattaaa
     8281 tgcaaaagtt ataaatgcag ctgataaagc tgaagaacta ggaaattcaa aaataatgaa
     8341 tgttatttta ttaggatgtc tagttaaatc tatgcaatta gagtctattg attgggaaaa
     8401 aattataagt gaaaatgtta aaccaaaatt tgttgaatta aacataaaag ctttccatga
     8461 aggtatggaa atggttagca aatagtttat aatagatata aatagactat agaaattaag
     8521 gtgaaaggaa gaaaattatg acttacagaa tattagccat aaatccaggt tctacttcta
     8581 caaaaatagc agtatatgat ggagaagaac aaattcttgt gaagacgata gaccatccgg
     8641 ctgaagagat tgcaaaatat aatactatac aagaccagtt tgaaatgcgt aaggaagcag
     8701 ttttgaatat tcttaaagaa aatagtatag acttaaaatc tcttagtgca atagtaggaa
     8761 gaggtggagt tttaccacca gtaaaatcag gagcatattt agtaaatgaa gaaatgattg
     8821 atgtactaag acatagacca gtacttgaac acgcttccaa tttaggtgct gttgtggcac
     8881 atgcaatatc agaacctctt ggaatcaact catatattta tgattctgtt gcagtagatg
     8941 agcttataga tgtagcgaga atatctggac tttgtggaat ggatagatca agtgcagggc
     9001 atgcattaaa tactagagca atggctttaa aatatgctaa ggataaagga aaagattata
     9061 agagcttaaa cttaatagta gctcatattg gtggaggagt aagtatttat cttcatgaaa
     9121 aaggaagaat ggttgatatg ctatctgatg atgaaggacc attttctcca gaaaggtcag
     9181 gaagagtacc tgctacaaaa ttagtggctg cctgttattc aggtcaatat tcagaaagag
     9241 aaatgactaa aaagataaga ggtaaaggtg gtatagtttc atacctaaat actgtagatg
     9301 ctagagaagt tgaaaaaatg atagcagaag gaaatgaaga agcaaaaatt atttatgaag
     9361 caatggctta tcagttagca aaaggtattg gagagttagc aactgtagta gatggaaagg
     9421 tagatgctat aattataaca ggtggaattg catattctga aatgtttact tcaatggtta
     9481 aaaagaaagt tgagtttata gcaccagtag aaattatggc aggagaaaat gagttggaat
     9541 cacttgcttt tggaacttta agagtactaa atggagaaga agaagctaga atctatagtg
     9601 aaaattaata agaaaaacta aaaatgttta tatactaaaa aagtggtata tccgtttttt
     9661 atacacgaaa gcttagagta taggtgaaaa tgtataaaat ttggattgcc actttttata
     9721 attaaaattt tatcttaacc taataaataa ttctgtaaaa tttcgtattt atatatatat
     9781 tttattggta tttattgtta ttattattat gaggagtgat ttttgatggg aaaaataagt
     9841 ttaaaaagta tgataagatt attttcaggt ttttttatat ttgcaatttc ttctgtgctt
     9901 atgattaatg cacatgtagg tcttatgcct tgggatgtac ttcaccaagg tttatctata
     9961 aagctaggca taactatagg tcaggcttct ataatggtag gagttgtaat tgttatattg
    10021 gatgcagttt ttggtgaaaa tataggatgg ggtactttac tcaacatgac atttatagga
    10081 atttttattg atttagtaat attttctgga gtgatacctc atgcaagcaa cacttatata
    10141 ggtgtgttta tggttgttat aggaattatt ttggcagcta tagcaagttt tctatactta
    10201 ggtgtttgtt taggaagtgg accgagagat gggcttatga ttgctcttca gaaaaaaact
    10261 aataaatctg tacgtcttgt tagaacaatt cttgaaatac tagcattagt tgttgggtgg
    10321 ttattaggag gttctgtagg tattggtacg cttgtaagtg ctttaggttt aggatatgta
    10381 ttacaaatag tgtttagaat atttaaattt gacacaaaat tgctgaaaca tagatttatt
    10441 atagatgata taagagaatg gaaggaaaag aaaagcaatg aacataaatg taaatcaagt
    10501 attgtaataa aaaatgagca gaattaatta tgctaaaata tatttgtagt ttatctaatt
    10561 tatagtaaag tcaaggagat tttgaaatgg aattatggga tttatataat gctgatggaa
    10621 ttaaaacagg aaatgttata aaaagaggta attctattga agaaggttat taccatttgg
    10681 cagttgaagt gtggatttta aatagtaact ctcaaatttt aattcagaaa aggtctaaaa
    10741 gtaaaaagac attgccaaat atgtggggaa tgacaactgg atgtattgtt tctggggaag
    10801 acagtttaga aggagcaatt cgcgaagcaa aggaagaaat agggatagat ataactaaag
    10861 atgagatgaa agtttttaga agtatgattc atgaagatac tctttgggat gtatacttag
    10921 ttaaaaaaga atatgatata tctaaagcta ttttacagga ggaagaagta agtgatataa
    10981 agtgggtaag cacagatgaa ataagacaat tactaaaaga gggtttgttt tttgaatatc
    11041 cagagatata cgaattgcta tatgagatag ataataataa attaaatata tgatgtagag
    11101 cagatttcag tataagtaag attatttaaa atgtatcttc tctaaatttt aatattgttt
    11161 tatgaaaatt aataagttat ttttagtatt tttctacttg ttttttgaga ttaaaataaa
    11221 aagggcagtc attataaagt cataaaaatt tggggaggat ttaaactatg gaagataaca
    11281 aaggattggc acgtgacagt tcaaaatata aaattgtttc tatatctgtc gtagtaatta
    11341 ttctaattac attttggtat atgttaaata tggtgctttt aacatttatt atgactttta
    11401 ttttctataa cttgcttgta gccacaagaa aacgaattaa gaagttttct tctttaaata
    11461 ttccagattc attgataatt atagtgttat atgcactatt tgctatattg ttagtattga
    11521 ttagctatgc agttgtacca ataattataa ttcaacttac agagttgagt agggttttta
    11581 gtgattttga tgtaaatcaa tttgctcaat ctttaggacc aaaattgtat cctattgtct
    11641 caaagctgga ttttaataaa tatatttcac aagcagggct acttatagca tcaacagcaa
    11701 caaaagttgg aagttttgga gtaaatatat tattggcatt tttactgagt ttactccttt
    11761 tacttgaaaa aaatgaaatt aaaaattttg gagataaact ttctgatagt aagatttctt
    11821 ttatttataa ttcgcttgta ttttttggaa agagctttgt taaaaatttt ggtgaagtaa
    11881 tgaaagtaca agttatgatt gcctttataa attctgttgt ttctatgatt tttttaggtt
    11941 tcatgggatt ccctcaaata tgggctttag gatttatgat ttttgtgttg ggtcttattc
    12001 cagtagcagg agttatagtg tctttgattc cattaactgt aatagcattt aatacaggag
    12061 gaataacaaa ggtttttgga gttttactta tgatttgtat agtgcatgca gtagaaacat
    12121 acatactaaa ccctaagttg atgtcgaata gaactaaact tccagtttgt tttgttttta
    12181 ttattttact tgtaggagaa cattatttag gtgtatgggg attactaatt ggagttccaa
    12241 tatttatgtt ccttatggat attctgggtg ttaaatttac ttctaggtaa tatattaaaa
    12301 taataattat tttttatata taaattatga attgttttaa aaaagaacct ataaatagac
    12361 atcattaata aagacatcta tttgataggt tcaattttgc tttgtataaa tactataaaa
    12421 ctaaataaaa agaaatttat ttcatttcaa aagtttcaca acttgcattt ctttcattta
    12481 tatgaacttt ttcagctttg caaagttcat ttttattgta tttacatttt acagcttcac
    12541 aatgaatgtt atctgtatca acttggttag tttcattaga acgactagtt ataccagatg
    12601 tatatttgtc ttcaaaagaa gcacaataag tattttctgt agtagttgct tgcattccat
    12661 ctacttttat actaccagca taacaatatc cactagtatt ataagcacaa ttagtagcag
    12721 cacaatttaa attattattt ttcataaata acctcctaag ttttatttaa acttattatg
    12781 tgaagttatc taaattttat acacttattt acaattacat ttatcacatt taacaactac
    12841 atctttggaa gtttcccttc ctaaattttt attaacatga tacacatcaa cattttcagt
    12901 tgatccacat atagaacatt taaaattgtt ttctagtagg gttctttctt taaataactt
    12961 ccaatacata gaattgatgt aatcattata atttaccttg aggcatgtct caagaaagtt
    13021 tatgtataga ttgtctaaat ctcctgtatt taattctgta tattctggtt caaaatattc
    13081 acaacatcct ctaaaatcgg gctttgtatc tgaaataaca catctaatat catatctata
    13141 gtctaggcta ggccccttaa atgaaagatg tgtgcaaaaa gaacaagatg tttctggtag
    13201 atttttttca agttcatgta atttattatt cataagtatt cctccaaaaa atttatttta
    13261 agtatataaa cgaatagatt attttaaaac ttttatttat ttatttatat tagagattta
    13321 ggataaatat tccaataagg agtaaatact taagtattta ttactgtatt aaataaaaaa
    13381 gaggcaaatc taacttgaga ttgcctactt ttttatcatt ttttaataaa atttattcta
    13441 agtattattt tatttagatt tagtcagcca acttagtatc tgctttgctt tttaactttc
    13501 taccagagcg tacagttaaa atagcatata atacagtaaa tatggcagct aaaacatatg
    13561 atatatttat gtttaagtta aatccaattt gagcattaaa tatataactg aatataacaa
    13621 aggaataaaa cataccagga attaagctta taatataatt cttttctttt attataaggt
    13681 aaactgtaat cattgcaaat gcaaatatgg caatagtttg attactccaa gagaagtatc
    13741 tccacaatat attaaatcca gaagcactaa gctttgcaaa tataaggatg gctatgacag
    13801 gaataaatat acagattgac aatataacac gatgtttctt ttccttttgg tcataatgaa
    13861 agtaatctgc aagcattaat cttaaagatc ttaaagctgt atctccagaa gttattggta
    13921 aaacaattac acctattata gctatgatac caccaataga accaagtaaa tctcttgcaa
    13981 ccaaacctat tacatcagga gaacctacta attctttagg tattccttta ttgtaaatac
    14041 ccatagcagc tgcagcccaa atcattgcaa ttaaaccttc taaaatcatc atattataaa
    14101 aagtagtttt tccttctttt tcatttgaaa ctgatcttgc tataagagta gcttgagttg
    14161 agtggaatcc agaaacaata ccacaagcaa ctgtaacaaa gaatgtagga ataagaggaa
    14221 taccatcagg atgaatacct ttccaatttg caagacttaa attagctaaa tcataacctt
    14281 gtgtaaagat tccaattcca acaccagcag cagataaaag tagaatagct ccaaatattg
    14341 gatatacctt tcctatgatt ttatcaattg gaaatagtgt tgcacacaag taatatagaa
    14401 agataagtcc ataaactatc cagacaactg gattatttat agtcgatttc atatttaaaa
    14461 tttgagtaac aactaaatcc ccaggtgtat aaataaaaac tgcaccaact aaaagcatta
    14521 aaagacaaag gaatatatta tagacttgaa atactttatt acctaaaaat ctactgataa
    14581 gagaaggcat ttgtgctcct ttttctctta tactaatcat tccactcata tagtcatgca
    14641 aagctcctcc aaatacacag ccaataggta ttagtatgaa tgctattgga ccaaacaata
    14701 taccttggat aggtcctaga attggtcctg taccagctat attaagcaac tcaattagac
    14761 tatttttcca ttttttcatt gggacatagt ctactccatc actttgagca agtgcaggtg
    14821 tttttctgtc atctgggcca aatacttttt cacaataagc accatataaa gcacctccta
    14881 ttagtaatat agccaagccg attaagaatg ttgtcataat atcaccccat ttataaaatt
    14941 ttttggtctt tgtatacata ttatctaaat gtgtagattt atattgaatt taattctgga
    15001 caacctacaa attctataaa gtattacaac taatataatt tcagaaataa atacatatac
    15061 ataaccatgt acttagtata caggtatttg gtaatatttt caatgttata gcaataaaga
    15121 atataaacaa aattctgata atattttaca aaaaaattaa aaaatataaa aattagttta
    15181 aatagatgga gaaaaaagga aaatcaattg agaatataaa tgtctatatc ttaacaatat
    15241 taagaggttt aaaattcttg aagaaagaat agtcaagtga tataatgagt ttacaggtgt
    15301 atatacacta gtacatatag aaagcggtgc aattatggat aaagatttaa catatcaaat
    15361 caaagagcta aagaaagaaa aaaatgcaat aatacttgcc catttttatc aaccaccaga
    15421 aatacaagaa ttagcagatg cagttggtga ctcatattat ttaagtgaga ttgctagaga
    15481 ttgtaaagaa gaagtggttg tattttgtgg agtaaggttt atgggagaaa gtgctaagat
    15541 attatcacct gaaaaaactg tattgatgcc agtaagtaac gctgggtgtg ctatggcaga
    15601 tatggtagat gaagaaggcg tgataaaatt aaaacaacag tatccaaatg ctttggttgt
    15661 atgttatata aactctacag ctaaagttaa ggcacattgt gatgtttctg ttacatcttc
    15721 tagtgcaatt aaaacactag aaaacataga taataaagaa attatattcc ttccagataa
    15781 aaatttagga ggatatattg cagaacaatt tccagataaa aactttatat tttgggacgg
    15841 atattgtaaa taccataaca atataagagc tgaagaaata attgagttaa aagataagta
    15901 taaaaatgca gaagtattag tacatccaga atgtaaaaaa gaaataagag atttaggaga
    15961 ttatgtaggt agtacatcag gaataataaa atatgctact aatagtaaaa acaaagactt
    16021 tataatagca actgaagaag gtatattaca tgaattaaag aaaaataatc ctaataaaaa
    16081 cttttatatt cctggtggaa aaatactatg tacagatatg aaaaagacaa cattagaaaa
    16141 tttatatagt acacttaaaa atatggaaaa tgaagttatt gtagaagatg aaatcatgga
    16201 aaaggcttta aattcactgc ttaacatgca caaattagca gaaggataga gggatagact
    16261 tatgaattta gaacaagatg ttttaatagt tggaagtgga gtttcaggat tatactgtgc
    16321 attaaattta gataaaagtt taaatgtgct agtagtatcg aaatcaacaa ttgaaaacaa
    16381 caatacatat cttgcacaag gtggaatttc aactgctaga aatattgatg atatagaatc
    16441 ctttgtagaa gatacaatga aggctgggca gtataaaaat agagttgaag cagttcaagt
    16501 acttgcagat gaatctatag aaaatgtagg tcagatagta gaatatggta tgcctttaga
    16561 caaagaaaat ggagagatag attacacaag agaaggagca catagtgtaa atcgaatagt
    16621 tcatagtaag gataatactg gagaagttgt ttttaaaact ttattaaaag aagctaaaac
    16681 tagagaaaat ataactctta tagaagatgc atatctttta gatatattaa aagatggcaa
    16741 taaatgtata ggagctagaa tttttaaatc taaaaaagag attcatgttt tttcaaaaat
    16801 agtagtttta gctacaggtg gaattggtgg gctttttaaa aattctacta atcaaagaca
    16861 tcttacaggt gatggaattg caatagcttt aagaaataat ataaaaatag aaaatttaga
    16921 atatattcaa atacatccta cagcctttta tgaagaaaat aatgaaggta ggagaatgct
    16981 tatatctgaa tcattaagag gagagggagc taaactttta aataaaaata aagaaagatt
    17041 tgtggatgaa cttcttccta gagatgtagt ttcaaaagct atttttgagc aaatggaaaa
    17101 agataaatta ccttatgtgt atttagatgc aacacattta gatagtgaat atttaattaa
    17161 tagattttct tttatttata atgaatgttt agctagagga acagacataa cgaaagaatg
    17221 tataaaggta tctcctgcac aacattattt tatgggcggg atacatgtag atttggattc
    17281 aaaaacatct atggaaaatt tatatgcagt tggtgaaatt agttgtacag gtgttcatgg
    17341 agccaataga ttggcaagta attcattact tgaagggctt gtattttcaa aaagagcagc
    17401 taaaaatata aactcagtta tagataatgt aaaagttaag tttatagatg taccagatat
    17461 ggatatagat atagaacagg taaaaaaaga aaataaaata ttagtcataa aagaaattga
    17521 aagaacaagt gaggattttg gcgatgaatt atttgattat tgatagaatg atacaggatg
    17581 ctttgataga agatgttcca agtgaagata taacaactaa ttctattgtg gatgaaaatt
    17641 ctaaatctac tgtagattta atttgcaaac aagatggtat aatagctggc ttaggtgtat
    17701 ttaagagagt ttttgagata cttggtgatg tagatgtaaa attatataaa aatgatggtg
    17761 atagagtaaa gaatcgtgaa aagatagctg tattaacagg tagtactaga aacttacttg
    17821 taggagagcg tgtagcattg aattatttac agagaatgag tggtatagct actattacaa
    17881 gtaggtatgt tgaaaagctt gaaggtacaa atacaaaatt attagatagt agaaaaacga
    17941 tacctaatct tagaatatta gataagtatt ctgtcaaagt aggtggagga tgcaatcata
    18001 gatttaattt atctgatgga atattgctta aagacaacca tattggggct gctggtagtg
    18061 ttaagaaggc agttgaatta gctagaaaaa atacttcttt tgttagaaag atagaagtgg
    18121 aagtggaaac tttagacatg gtaaaagaag caatagaagc taatgcagat ataataatgt
    18181 tggataatat gagtttggaa atggcaaaac aagcagtaga tattataaat ggaagagcca
    18241 ttgtagaatt ttccggaaat gtaaatttag atactataga agatatagga aaaattggtg
    18301 tggatgttgt ttctgtagga gcgcttacac attctgttaa agctctcgat ataagtatga
    18361 agaatttgag aaatatataa attttatatt gaaataaact taattaaaat agactcctta
    18421 aatttgtttt ataaatttaa ggagtctatt ttttattcat taaaattaat aaaaatagaa
    18481 aaaaatcaat caataattta ataaaaatag ataaatacta tatacacttt taataatata
    18541 gttaatatct attttacaga taaataagaa taaacttata atataattgt atctgtaaaa
    18601 ttttgtaaaa tataaaaagt atagggggaa taaaaatgaa agtagcttat atttttagta
    18661 gtactaatac acataaaatt ctagacaaaa tgataatacc acaacttgaa caaggaactc
    18721 atggagtaga cgttctaggg atgtttttct ttatggataa taccttattt ttatctaaag
    18781 gtaatcctgt aggcgagaga ctaagtaaat tacatgaaaa aactgatatg ataataatgg
    18841 cttgtgacca atgtgctata gaaagaggta tagagaataa tttggttgat ggagctacaa
    18901 ttggatgttt cccaaaccta tatgcttgtt taggcagtgc aggtgtggac caagttataa
    18961 cgttataaat tagtatagga tacaatccat agaaaaattt attttctgtg gattatttta
    19021 agtaaaagat aaattgttta aagtagggga tgaacaaatg tctaaaattt tagttgaatt
    19081 tataaacact tgaccatctt gtgatgaaca tggagtgttt ataaaaaaaa tgtcagaaaa
    19141 gtatgctgat aaattggaaa taaaattata tcaagcaggg aaagattttt cgtatataaa
    19201 aaaatatggg attataacaa aaggaacttt aataataaat cagaaaaaga aatatgatag
    19261 attaaataaa gatacaattg aaagggcaat agtagaagct ataaacaata attaaaagtt
    19321 taaaaattag gagtatcaaa aatgaatata ttagaaattg ttttaaatgg agttaaagat
    19381 actatagtag cttctatagt tacactgaat ggagcatcag gatggcttat gataagtttt
    19441 atattggcag gtgtacttca caatgtttta actccaagaa ggtttcaaaa acaacttgga
    19501 aataagaaat tttcttcttt aattaaaagt actatctcag gaatgttttt accaatatgt
    19561 agttgtggag taataccact tggaataagt atgtattatt caggagcata tttaggacca
    19621 gtacttgctt ttatgacatc aactccaata ataaatccaa tagctgtagt tctttgtcta
    19681 ggtcttttag gtcctaaagt tacaatcatt tatctaatag ctggtttttt agtaccattt
    19741 ttagtaggat ttttaggaaa ttgttttgga aaagaagaat tatcacttca taatgattca
    19801 gaggaagaag taatagaatt agaagaagaa aaaagaagtt ttatgggaaa tatgattgat
    19861 ggtcttaaat ggtcttttgg tgaattagcc cttactatta gtaaatatgt agtaatgggt
    19921 atggtttttg caggatttat aacaactatt tttccaaata gtataattca aaaatattta
    19981 ggaaatccag gtttgctatc tcttggaagt atagcaatac ttgcatgtgt tatgtatgta
    20041 tgtgctgtag gacatatacc gtttatagca gcccttgttg caagtggagc atctcctggt
    20101 atagcaataa catttctaat agctggagca gctacaaatc taccagaact tataagtatg
    20161 tttaaactta ttggaaaaag aactgttatt atatattctg taactttgac actgtcttca
    20221 ataataattg gatatataac aaatttactt ttagtgaata ataactcagt aaatttagat
    20281 gcaacaaatt cttcaataca gttggcaaat aaactaatgt taaatatacc tgatagtttt
    20341 gagtatgtat gtactatttt aattatttta tttgcattta aatcaataat accaaagatg
    20401 aaagaggtca taggaaatca tgcttaaaaa gatagttata atatcctgta tagtagttgt
    20461 gttaatcatt ttatcaaaaa tagttgatga taacatcaaa gaagatgctt ctattccaaa
    20521 tgtgaataaa gaaacattag aatattttag aaaaaattat aaagaagaca taataacttg
    20581 tgctgaagaa gacttaaata atgatggaaa aaaagattta gtagttatat ataaaaaaag
    20641 caataattca aatgaaatgg tagtcgtagt ttctgataaa aatagccact atataacaaa
    20701 gccaatacca gcacctatag agaatcaaac gataaccttt aaaaatattg atgataaagc
    20761 accaatagaa gtaattgtat cagggtctaa aaatggtaat gtaggatatg ctatttacag
    20821 agtagagggt aaaaaatttg ttgatttatt tggcgaagat atggacaaat gttgctaaaa
    20881 ataaaaataa tacataaagg tgggaaattt atatgaaatt atctaagaag attcaagcat
    20941 taatcttatt tggtgtaatg ggtacatcta tattaacagg atgtagtagt aaaccaaaag
    21001 aaaaagaaga agcaaaaact ggaaaagatg attatacaat aacagtaggt tattataatt
    21061 gtgaccatat gactgcggga ccagtagcag aatcggcagg aatatataaa gatttaggtc
    21121 ttaatgtaaa aactgttgga aatggtaaag ttcctgaagc tatggcagca ggaaaaatgg
    21181 atgcaggtta cataggaact aaggggttag ttggagcaat acctaagggt tcgccaataa
    21241 caatagcagc taacaaccat actggtggtt ctgaatattt aatagtttca aaagatataa
    21301 aagaacctaa agatttaata ggtaaaaaaa ttgcaacaga tatgagtgat ttcttatgga
    21361 caagtgatta tggtccagaa acaggtctac caactgaccc atcaaaatat gaagttgtaa
    21421 atatggattc agacaaagat aaatatttag ctcttaaaac tgggaaaatt caagctttta
    21481 catcttgtga cccttggggt tctgtagctg aaaatgacgg tgcaggaaaa ataatagcat
    21541 ctactcaata taaagaaaaa gctaatggca aagaatataa ctgttgttca ttctcattaa
    21601 acaaaaactt tataaaagaa catccagatt tagcaaaaaa attggttcta gcacatacaa
    21661 aagcaataga atatatatat acaaatccag ctgaagcagc taaaatattt gcaaaatatt
    21721 ataatgtaga agaagaagtt gcacttagaa ctatctataa gaagactgtt ggagaaggta
    21781 gaactcttac ttggaaagtt acaggtgaag aatataaaaa taatttacaa atgtataaag
    21841 atttaaaggc tttagatgat atacctaaat atgaagatac gattgataca agcttattgg
    21901 actcttgtgg agctgatgat tttgacaaat ttataaaaga aaaagttaac ccagagtttc
    21961 ctgaaggtat gagttatgaa gactggaaag caaaggtatt ataaataaat ggagaatatg
    22021 aaaaatataa taatactttt tatcattatt ttgggaagta tatttataat attttttaat
    22081 tttgaagata aagaagaaat aaaaggaaaa ttaagaatag gggtatctga tgatacctct
    22141 ggttttgtaa taaattatat ggtaaataga gactactttg aaaatataaa agtaagtgat
    22201 ataatggata tttttacaat taatgattgc tgagcaagta caatgcagtg ggctctgagt
    22261 tcagaggaaa tagatatagc aatcatatgt aaagatgctg caaaacaata tgtaaatgta
    22321 aatagtggat ttgaaattgt tggtactgta gttcaaaatt ctgatatatt tctattaagt
    22381 gataataatc ctgaaaagat aggagtcatt caaaatagag attaccagtc agaattggtt
    22441 aaaaaatact acaagaatgt ggaagtagct cccttattag ggactgcact agcatatgga
    22501 ttagaaagta atttagtaga tggtgttgta atagattgca taaagtcaat tgggttagag
    22561 ggtaaaagaa aatctacaac taaattagga gattatgata cctatgtatt agttgtaaat
    22621 aaagagttta aaaaaagtaa agcatatagt gattttataa cactttacaa taaaagtgtt
    22681 gatgctttaa agaataaagc tacatttaag aaagtattaa aagagtacag gaatataaga
    22741 aatatagata tggaggaaat aagcaattgg aaattaaaat ttctaaagat aaaagcgata
    22801 gattaactaa gaaaaaaact gatggtagta tggatatttt atataaaata atactattag
    22861 ttattatatt tttagtctgg cattttgcag caaaagatat agggagttct ttgttacttc
    22921 caatgccagt agatgtaata aaaggtttct ttttctgtgt gacagatgca gagacagtca
    22981 ctaatctttt tataacattg cagagagtac ttaaaggttt tatgtatgca ttattgtttg
    23041 ggcttccaat aggatttatt atgggatttt ctaaaacttt tgaaagggtt ttatctccag
    23101 tagtagattc tgtaaggcaa gttccaataa tggcatgggt tcctcttact atagtatggt
    23161 ttggtatagg agatggtcca acaatatttt taatagcttt ttcaggggtt ttccctataa
    23221 ttttaaatac tattcaagga gtaagagcaa tatcaaagga ttattataat gcagctagaa
    23281 gtatgggagc atcgccaatt gtgatattta caaatgtaat tgttcccgca tctcttccag
    23341 atatactaac tggttcaagg attgctataa gtacaggatg gatgtctgtt atctgagccg
    23401 agtttatagc tacgagtgcc ggtctcggtt actcaatggt acaagctcaa acattaatgc
    23461 atacagatgt attaattggg cttatgatat ttgcagcttt aataggattt ataatagata
    23521 gggtattaaa gtttataaat aaaatccttt gtagatggag gtttgcagat taatgaagtt
    23581 aagtgtaaga gatataaata agacatttgt aaataataga gtacatacaa aggttttaga
    23641 agatataagt atggatatag atgatggaca atttgtctgt cttttaggac catcaggttg
    23701 tggaaaaaca actttactta ctataatagg tgggtttcaa aaatcagaac gtggagatgt
    23761 ttttataaat gataaaagag ttaaaaaacc gggcatagat agagctttta tatttcaaaa
    23821 ttatgcttta tttccttgga aaactataag aggtaatgtt ctatttccta tgaaacagca
    23881 gaaaatacca aaagaaaaaa gagaagaaat gctagaagaa cttttggtta tgtcagactt
    23941 gaaaggaaaa gaaaaactgt ttccgcatca attatctgga ggaatgaaac aaagagtggc
    24001 catgataaga gctttagcat gtaatccaga ggtattacta atggatgaac cattaggagc
    24061 tgtagatttt caaatgagac aaaatttaca ggaagagcta gaacgaatat ggataaagaa
    24121 gaaaataact gcattaatgg taactcatga tgttgatgaa gctgtatata tgagtgatag
    24181 agttatagta atgtcgagag ataaaggaag aattattgat gatataaata tagatattcc
    24241 aagacctaga attagaggaa gccaaaaata tgaagagtac aaaaacaaat taactgatac
    24301 tttatcaaag tgttatgaag tttaaagagg agtagacatg agtcaaatag aaaaatacaa
    24361 tagtttggat aatacacaac agaaaataag aatctctatt atggatatga taatagataa
    24421 aggttctagt gttacacttc aagaagtgac tgaatatgta agtaaaaagt tgaatataga
    24481 aaaagaatat atagaaagga cactacaata ttttatttat aaaaatataa tggttgttga
    24541 tgggaatagt ataaatttta tatatcctgt atcagcattg ccaacaaatc ataaagtaac
    24601 tttgagagat aatagaagtt tcagtgcaat gtgtgctata gatgctatag gaacctcttg
    24661 cacattcaac caagatataa aaataaactc tatttgtagt gctacaggta aggaaataga
    24721 agtaattata aaaaatgaaa agattgagta tgtgaataat ccaaacttaa gagtgcttca
    24781 tataaatctt gataagcatc taaattgggc tgctagttgc tgaaatataa tgaatttctt
    24841 ctggacgaag agtgattttg atgcttggac aaacgaagcg ggattatctg atgatgaaga
    24901 tatatattgt ttagatatta atgaggcaat agtagaatca tataaaatat ttaaactcaa
    24961 acaaaaggta ctatcataga tatacatata tgaaaaacta aaaatgacct ttagataaaa
    25021 atgataggta atatccatac tatataaatt agtatagagt agggatgtga aaagtattta
    25081 aatctaaagg tatattttta gtttttttag atgattagat agccttatta agtatatatt
    25141 tatttataac ctttgcaact ccattttcat tattagtatc agtaacataa tctgcaattt
    25201 cctttatact attacgtgca ttccccatag ccacaccaag acctgcatac tttatcatat
    25261 gttggtcatt ttcttcatcg ccaacagcaa tagcattttc tataggtata tttagattcg
    25321 tacataatgt tttaagagca gttcctttat tagtagtttt acctaaaaat tctagataaa
    25381 aaggagcaga ccttactata gtatatttat tgaataaatt tttaggtagt tgctgtatga
    25441 tttcttccaa tctttctggt tcatcaacta tcataatttt acagatttta atagattcat
    25501 caattttatc tttttgtata tatttgactt ctatattatt taatttcgct tcatgaagtg
    25561 tatatttgcc aggcgtttca tttggtgtaa ttaaatttgt cattgtattt atgtgtattt
    25621 tagtcttata ttttttacaa acgttattat atataaaatc aaaatcatct aaactcattt
    25681 ctacatcatg tatgattttc tcacttttaa cttcttgaat aattgctcca ttaaagcata
    25741 taacataatc accttcatct gttaaattta gttgatttag aagttctttt acaccaggaa
    25801 gaggtctgcc tgttgttata actacttttg ccccagccct ttttgcctcc tgaatggact
    25861 caaagacttc aggagtaatc cttttttgtg tatttaatat tgttccatca atgtctagtg
    25921 cgattaattt gtacatatca acctccaata aattatatac tcaaatttcc tgtgaaaacg
    25981 ttgacacagc tagtgtcgaa caaagtttca gtgggaaata tggtttgttt tgtatactat
    26041 tatatcaaga gataagtaat ttagaaagtt taataatatt aattttttga taaaaatatt
    26101 acttatgtga gatgagaatg taaaaacttt gtaatcaata attccaaaat tcttaattac
    26161 aaataatggt atcgtttgca ttgttaatat aaataaatgt atattatata gttagaagtt
    26221 tgaataaata gactgatggt aatttgttat attagttagt tttgtgttat aaaatattgt
    26281 aattccatta atcatatgtt attattcaac atgattataa tataaagaat atttgatata
    26341 tataataatt tataaaagag tattacatag tacatgtaaa tctatattta taaatacaat
    26401 aaagtaaagt tgtaaaggag tgctttaaat gaagttttca gagtttaagt atgaaagacc
    26461 caattataat agtatgaaaa aagagttttt gagttgtgta gaagatataa ataattctcg
    26521 taattataaa gagcaacaaa aaaatataca taagattaat ttattaagaa ataaaataga
    26581 aacgttgtca aatatagctt cgataagata tagtacagac acatttaata aattctacaa
    26641 agaagaaaaa aattattggg atgaatatat gcctttgtat gaagaattaa attcatattt
    26701 ttataatgcc attgtaaatt caaaatttaa atatgatttg ataaaagagt ttggagagca
    26761 atttttcaca atagtagagt attctttaaa aagtttttcc aaagaaataa taagtgaatt
    26821 gcaggaagaa aataaattgt gttcagagta tactagactt ttagcttcag ctgagataat
    26881 gtttgatggg aaaataagaa atttatctgg tatggggaaa ttcatgtatt ctaagaatag
    26941 aaaaacaaga gaattagcaa acaaagcata ttataatttc tttgaagaaa atgaaactaa
    27001 atttgatgat atatttgata aattggttaa acttagagat aagatgtcta aaaaattagg
    27061 ttttgaagat tttgtagagc ttgggtatgt tagaatgatg agaagtgact acagagaata
    27121 tatgataaaa aatgttagaa aacaagtact aaaatatgtt gttccaatgg caaatgagct
    27181 ttatgaaaaa caagctaaaa gaattggtct agaatattta agttacatag atgaaggcgt
    27241 ggaattttta acaggaaatg caagtttaaa gggagattca agatacataa ttcaaaatgg
    27301 taaaaggatg tattctgaac tgtcaaaaga aacaagtgaa ttttttgatt ttatgttaga
    27361 aaatgaactt atggatttag aaacgaagaa aggaaaagga gctggagggt attgtactta
    27421 tataccagat tataaatcac cattcatttt ttctaatttt aatcaaactg ctgatgatat
    27481 agatgtgtta acacatgagg caggacatgc atttcagttg tatatgtcta gatggattga
    27541 tatgccagaa attaattttc caacgcttga tagttgtgaa atccattcaa tgagcatgga
    27601 atttataaca tggccgtgga tggatttatt ttttaaagaa gatacagata agtacaaatt
    27661 cactcactta tcatcttcaa ttaaatttat accttatgga gtaattgttg atgaatttca
    27721 acactatatt tacaaaaatc ctaatgtaga taagtcaaaa cgaaaagaaa tctggagatt
    27781 tttagagaaa aaatacttac ctcatagaaa atatggtgat aattcttttt tagagagagg
    27841 ttgttggtgg tttaaacaag gacatatatt taaaaatccg ttttattata tagattatgt
    27901 attagctcaa atttgtgcgt tacaattttg gaaaaaaatg attcaagata gagatgcagg
    27961 atggaaagat tatataaata tctgtaaagt tggaggaaca aaatcatttt tagatattgt
    28021 gagtatggga aatttgtatt ctccatttga tgatggctgt attgagtcta ttataggtga
    28081 tgttaaatct tggtttgatg aaataaatga cagcaaatta taaaaaatta tgagaatttt
    28141 attttataaa agtgaaaaat taaaaaatat ataaaaagtg attatttgtt gtgaaaatag
    28201 tcttgttttt aactaaattg ataaaataaa ctaaaaaaga tttgtactta aaaaaaaaag
    28261 aaaatacaag aaatatgcaa tatcaacatt tggaaaaaaa agtaaaaatg tatacaaaat
    28321 acatataaaa aaactattgc aaattttata gaatgtttat atacttaagt caacagagat
    28381 agaaaaaagc tatttataga aaacaaagga taaattgctt aattttaaat tttctgaaaa
    28441 aaataataat ccggatgaag agtatgggag actgctttat aaaaagcgac cgaagggaca
    28501 agtttaatct tacttaccca aaagattaga tgaaattctc aggtaaaggt gactgtactt
    28561 ggacggggct ctgaaaatga ttttaacagg gaagacaaat tgggagattt gtttttcctg
    28621 ttttttattt atcaagtaat aaataaaagt ttgaaagaga gtaaaaaaat gataataatt
    28681 acgaataatc caaaagtaaa agaagaagtt caaggcagag aagttttgtt taaagacaca
    28741 acttatattg gaatcttaga agcaagtaga gatttgatac atgaaggtta tgaactttta
    28801 tctcatccac tctatgggag tgtaaagcca aatgaaacac cttacaggac tgttatacta
    28861 aagaaaggaa atcgtttgga tataaactct ttaaccttaa ttgaagaagc aatcatcact
    28921 gcaagtaaat ttcagaataa taaaaagact ccaaaatgga cagaaagtgt tcaagatgat
    28981 tttagagtaa ttgattatga tatattttat aatacaatac aaagaatgca gtatgaataa
    29041 aaaaatttgt taaaaagtta ttaaaacagc ctaaatatta aatctaattt aatattaaat
    29101 aaaatgagaa atttaattgg ggaggataag tcaaaatgga aaatgtgtat gacttagtaa
    29161 taattggttc aggaccagca gggcttgcag caggtcttta tggagcaaga gcaaagttaa
    29221 aaactctaat cctagaaaaa gacaagactg gtggacaaat tgtaataaca catgaaattg
    29281 caaattatcc agggtcagtt ccaaatgcaa caggaccaag tttaatagct agaatggttg
    29341 aacagtgtaa agagtttgga gcagaaatgc taagagacaa cattgttgat actgagctag
    29401 atggagatat aaaagtttta aaaggggaaa aggcagaata tagagcaaaa gcagtaataa
    29461 taggtacagg agcaacacca agaaagattg gttgtcctgg agaaaaagaa cttactggta
    29521 aaggggtttc atattgtgca acttgtgatg cagatttctt tgaagatttt gaagtatttg
    29581 ttgtaggtgg aggagacagt gcattagaag aagcaatgta tttaactaaa tttgctagaa
    29641 aagtaacaat tgtacataga agacaaggat ttagatgtgc taagagtgtt gaagaaaaag
    29701 caaaagctaa tccaaagata gagtttttat tagatacagt aattgaagaa atcaaaggtg
    29761 atggaatatt agaatcagtt gtatttaaaa ataaagttac tggagaaact catgaatact
    29821 ttgcagatga agaagatgga acaatgggag tatttgtgtt cgttggatta gatgctcaaa
    29881 ctgatttatt caaaggtaaa gttgatatgg atgaaaaagg ttacataata actgatgaag
    29941 atatgagaac taacatacca ggcgtatttg cagcaggaga ttgtagatct aagactctaa
    30001 gacaggttgt aactgcaact aatgatggag caattgcatc tatagtagct gagaaataca
    30061 ttgatgaaaa atttgggaac tagaatctgt aaattaagaa atagtgtctg taaatttaat
    30121 gaataatatt taaaattaaa ataaaaattt caagggggaa taagaaaatg ttagatttag
    30181 ataaggctac atttgaagaa gaagttttaa atgcagaagg ttttgtattt gttgattttt
    30241 ggagtgaagg ttgtgagcct tgtaaagctt taatgccaga tgttcataaa ttagctgaga
    30301 cttatgggga taaaataaaa ttctgtaaga tggatacaac aaaagctaga agattagcta
    30361 taaaacaaaa agtattaggt cttccaacta tggctatata caaagatgga gaaaaagtag
    30421 acgaagttac taaggatgat gctacagttc ctaatataga aaacatgatt aaaaaatatc
    30481 tttaatatgt tgataaatta ttaacattaa aagaaaaaat ctatttttat ctgaagataa
    30541 gaagaaaaag gtttttatgt ccaactttgt taaaaaaata attttaaata aaataatttt
    30601 tttacaataa atatttttta gtaataaaat aaagatttaa ggcataaaaa cacaataata
    30661 tatctttaaa aaaataagat aaattgtaaa atgatataat tttgagaaaa actcaaatgc
    30721 atatacacaa attaaataac aggaggtgca agtatgcgtc ttgaattagg gaaaatcttt
    30781 ataaaagata ttcaatttgg tgatgtaaca gaagtaaaag atggagtatt attcatcaat
    30841 aagcaagaga tgctacaaga aattggtgga gatgagcata ttaaatctat agatatagaa
    30901 ttagctcgtc caggtgaaag cgtaagaata acacctgtaa aagatgttat tgagccaaga
    30961 gtaaaagttg aaggtaatgg gggaatcttc cctggtatca tgtctaaagt tgacactgta
    31021 ggagaaggaa aaactcatgc tttaaaaggt gtagctgttg taactactgg aaaaatagta
    31081 ggtttccaag aaggtataat agacatgact ggagaaggag ctaagtatac tccattttct
    31141 aaattaaata atgtagtagt aatagctgaa cctattgatg gtttaaaaca atacgctcat
    31201 gaaaaagctg ttagaatgat aggattcaaa gcagcaatgt acttaggaga agtagcaaga
    31261 aatcttactc cagatgaagt ttcagtatat gaaactaaac ctcttcttga atcaataaaa
    31321 gaatatccag agcttccaaa agtaggatat gtttacatgc ttcagactca aggtttatta
    31381 catgatactt atgtatatgg agtagatgca aaacaaatag taccaacttt attatatcca
    31441 acagaattaa tggacggagc aattgtaagt ggtaactgtg tttctgcatg tgacaaaaac
    31501 ccaagttatg ttcatataaa caatggggtt gttgaagact tatatgcaag acatggtaaa
    31561 gatataaact ttgtaggtgt aattataact aatgaaaatg tttatctagc agataaagaa
    31621 agatcttcaa actggacttc taaattatgt aaatatttag gattagatgc agttatagtt
    31681 tcacaagaag gttttggaaa tcctgatact gaccttataa tgaactgtaa gaagatagaa
    31741 atgcaaggtg ttaaaacagt tatagttaca gatgaatatg ctggacgtga tggaggttct
    31801 caatcacttg cagatgcaga tgtaagagct gatgctgttg taacaggtgg aaatgccaac
    31861 caagttgtag ttcttcctaa gttagataaa gttattggac atttagaagt agtagatgta
    31921 atagctggtg gttctgatgg aagtttaaga gctgatggaa ctattgaagt tgaaatccaa
    31981 gctataacag gtgcaactaa tgaaacagga tttggtcatt taactgctaa aggatattaa
    32041 gaagtatttt attaaacgat tgagtaatgt taataaaaca tattatagat aattataata
    32101 agtgttatag atatataaaa atatattaga aatataataa ggaggattat tatgagttta
    32161 cttagtaata aaaaggttct tataataggt gaccgtgatg gtataccagg acctgcgata
    32221 gaagaatgtg taaaaacagt agaaggagca gaggttgttt tctcatctac agaatgcttt
    32281 gtctgaacag ctgctggggc tatggactta gaaaatcaaa acagagttaa agatgctgct
    32341 gataaattcg gagctgaaaa tgttgtgatt ttactaggtg ctgctgaagc cgaagctgca
    32401 ggtcttgcag ccgaaacagt aactgctgga gatccaactt tcgctggacc acttgctgga
    32461 gttgccttag gattaagtgt ttaccacgtt gttgaggaac caataaaatc attatttgat
    32521 gaaagtgtat atgaagacca aataagtatg atggaaatgg ttttagaagt tgaagaaata
    32581 gaagaagaaa tgtctggtat aagagaagaa ttttgtaaat tttaatgcct aaaagcaaaa
    32641 ggggtgaaac taagagatgg gaaaactaaa agcagtacat tatattaatc agttctttgc
    32701 tggtataggt ggagaagaaa aagctgatac taagccacat gtggcagaaa ctttacctcc
    32761 aataagttta caacttgata aattattagg tgaagatatt gaaatagttg gaacagtagt
    32821 ttgtggagat agctatttca atgagaatat agatagtgca agtgaagaag ttttatcaat
    32881 ggtaaaaggt tttgaaccac aacttttcat agctggtcct gcatttaatg ctggtagata
    32941 tggggttgct gctggaacaa taactaaagt tgtaaaagat gctttgaata taccagcttt
    33001 aacaggtatg tatgttgaaa atccaggagc agatatgttc aaaaaggatg tttatgtagt
    33061 agaaacttct gattctgcag caggtatgag aaaggcactt cctaagatag ctaaacttgc
    33121 agtaaaactt gctaatggag aagaaattgg aactcctaaa gatgaaggat acatagctag
    33181 aggaataaga gttaactact tccatgaaga cagaggttct aagagagctg ttgacatgtt
    33241 agttaagaaa ataaaaggtg aaccatttga aactgaatac ccaatgccaa actttgatag
    33301 agttgaccca agtaaagctg taaaagattt atcaaaatgt aaaatagctt tagttacttc
    33361 tggtggtata gttccaaaag gaaatccaga tagaatagag tcttcttcag cttctaaata
    33421 tggaacttat tctatagcag gagttatgga tttaacagaa gagacttatg aaactgcaca
    33481 tggtggatat gacccagtgt atgcaaattt agatgcagat agagtattac ctgttgatgt
    33541 tcttagagat ttagaaaaag aaggtgtaat tggaaaatta catgagactt tctatacaac
    33601 tgtaggtaat ggtacatctg ttgcaaactc taaaaaatat gcttctgaaa ttggagcagc
    33661 attagtggct gatggtgtag atgcagtaat attaacttct acatgaggta cttgtactcg
    33721 ttgcggtgca acgatggtaa aagaaataga aaaaactgga cttccagtag ttcatatgtg
    33781 tacagtagta cctatttcat taacagtagg ggctaataga atagttccaa ctatagcaat
    33841 acctcatcca cttggaaatc cagcgcttga ccctacagaa gaaaaagccc taagaagagg
    33901 ccttgtagaa aaagcattaa atgctttaac tacagaagta gatggtcaaa cagtatttga
    33961 aaaataaaaa taacacacac atatattata tagataaaaa tataaacaca cactaaaaat
    34021 aaaaaataac tatacacaaa aaaataatat agatttcatg aatggataaa ttggagttat
    34081 tcattcatga aatcttaaaa aatgccaaaa tacataaaca ataatagaat caaatataca
    34141 ataaaataca atgattagag cttttaataa gtttaataag attaaaaaat aagtgttcat
    34201 aaaatttatg gaggtgcaca aaatgactta tccagtttta aaaggggcag gctatgtact
    34261 tattcatact ccagatatga tagtgcaaaa tggaagtact tgtacagttg aaagagcaac
    34321 aaatccagat tcagaatttt taaaagaagt aagtaatcat ataagaagtt atgaagatgt
    34381 agttaactac atgccaaatc aagtttatat aggaaataga agaccagaag aattaagaga
    34441 tttaccaatg ccatggtgtg aacaaaagat tgagggaact agaaatggta agtttggaga
    34501 aataatgcct caagatgagt tcatagcttt aatgcaaata agtgatgcat ttgatttagt
    34561 aaaattatca caagaattta ttgatgaagt aaagccaaaa atcgaaaata actaccctga
    34621 aattgcacca tttgtaggta aattaaaggg tgatgatata gaagaaggta aagaattagt
    34681 agcaacacac atagctgaag gattatacca tgatggaaaa tttgttggtt atgtaaaaag
    34741 agcacatgat gtagatgtta acttaaatgc ccatacaatg tttgaaaatc tagtagtaaa
    34801 agcatctgga gttttatctg ctattcaaat gctaagacat agcaaaatag accctgctga
    34861 aatagattat gttattgagt gttcagaaga agcttgtgga gatataaacc aaagaggtgg
    34921 aggaaacttt gctaaatcaa tagctgagat agcaggactt caaaatgcaa ctggttctga
    34981 cacaagagga ttttgtgcag caccaactca tgcattaatt caagcagcag ctttagtaaa
    35041 agctggcata cataaaaatg taatggtagt agcaggtgga gcaagtgcta aacttggaat
    35101 gaatgctaaa gaccatgtta aaaaaggtct tcctgtactt gaagatgttg ttggaggatt
    35161 tgcagtactt gtatctgaaa atgatggtgt aaatccagtt ataagaactg atttaacagg
    35221 taagcatact gttggaactg gttcttcacc acaagcagtt atgacagcac ttataacttc
    35281 aggtcttgat agagctaatt taaaaataac agatgttgat gtgtattcag ttgaaatgca
    35341 aaacccagat ataactaagc cagcaggagc aggagatgtt ccagaagcaa actacaaaat
    35401 gataggggca cttgctgtta aacgtggaga cttagaaaag aaagaattga aagattttgt
    35461 ttctaacaaa ggattaccag gatgggcacc aactcaagga catataccat caggagctcc
    35521 ttatatagga tttttaatag atgatttaac tacaggaaat agaaatagag ctatgatagt
    35581 tggaaaaggt agtttattct taggaagaat gactaactta tttgatggag tatcttttat
    35641 agctgaaaga aatacaggtg ttactgaaga gacttcagga atatctaagg atgaaattaa
    35701 gaagataata gctgaatcta tgaaaaaact agcattagat atgttagaag aataggggtg
    35761 aggatatgtc aaaaaaagta atcgcagatg tattcttaga agtagctaat gcaatagaaa
    35821 gcggcgaatt tggcaaaaaa gtaaaaattg gtgttactac acttggaagt gaacatggtg
    35881 tagaaaacat ggttaatggt gcacaattag caaaatctaa tttatttgat attgtgctta
    35941 ttggaccaaa agtggaaact gaccttgaag tagttgaagt taatgatgaa aaagaaatgc
    36001 atgcaaaaat ggaagaatta ttagattcag gatatattga tgcttgtgtt actatgcatt
    36061 ataattttcc aataggtgta tctacagtag gtagagtaat aactccagca aaaggaaaag
    36121 aaatgatact tgcaacaaca acaggaacaa gtgctactaa tagaatagag gcaatggtaa
    36181 gaaatgccat ttatggtatt gctacagcta aatcaatggg taacaaatgc ccaaaagttg
    36241 gaatacttaa tgttgatgga gcaagacaag ttgagaagtg cttaaaagaa ttaaaagata
    36301 atggatatga catggagttt gcggattcaa ttagagcaga tggtggatgt gtaatgagag
    36361 gtaatgattt acttgtaggt gcacctgatg ttatggttac tgatacatta tctggaaata
    36421 tatttatgaa agtattttct tcatatacta caggtggaga ttatgaagct caaggctttg
    36481 gatacggacc tggagttgga gaggactatg atagaaaggt acttatagta tcaagagcat
    36541 caggttctcc ggtagtagca aatgctttaa aatacgctta tgatgttgta aagggagata
    36601 taagtaatgt tgcaagaaat gaatttgcaa aggttaaaaa agcaaaattt gatgacatta
    36661 tatcctctct tactaaaaaa gaagttaaag ctgaaaaagt agaagttaaa atgccagata
    36721 aagaaattgt tactcgtcaa atagctggtg ttgatataat ggatttagaa gatgcagtat
    36781 ctgagttatg gaaaaacgga atctatgctg aaagtggtat gggttgtaca ggtccaatag
    36841 ttttagtaaa tgaagctaaa ggagacttag cagttgaaac tttagttaaa gctggatata
    36901 ctgcaaaata agtacatttt ttatatgtaa ataagaacat atagttataa ttttattgat
    36961 tataattata tgttcttttt attttatatc ttgtcaatat ttatatatta acacaatcca
    37021 tttatttgat tctctagcta atttattata tttattcaat taaaattaag ctttctaact
    37081 aaataaaaac taatgaagta ttaaaatgaa attgataaaa tatgaaaaaa ttttaggaat
    37141 aaagtatctg ttagataaaa ataagattaa ttagtgatat aatataatta agataaaatt
    37201 tattatttgg aggtaaaatc atgagcagag taaaaaatgt agtagaattg ttggaaacta
    37261 aaggtgttga tgcactatat ctaacaaaga aaactaatgt aaactatata agtggttttc
    37321 ctgatgaaga agcatatgca gtgatttgca aagatggaaa ctttttagtt actgatagta
    37381 gatatatgga gcttgcagaa aaggtttgca aagattttga aattataaat tggcataatt
    37441 ttgatagaag tgtggcaaag gcagttaaaa gtgtatgtga taaagtgggt ataaagaaac
    37501 ttggatttga aagaactaat atagtgtttg ataaatatga agaacttaaa aatctgattg
    37561 agaaggataa tggagaatta atacctacag aaaatattgt agagactttg agatatgtaa
    37621 aagacaaaga tgaaatcaaa aatacaagaa aagcttgtga aatagcggat aaggcattag
    37681 aagaattgat tccacatata aaagctggag ttagcgaaat tgaactggct acaaggttag
    37741 agtactttat gaaaatgaac ggagcacaaa atatagggtt tgaaactata ctgatatcag
    37801 gagcaaagac ttcattgctt catggtaagc ctagtgataa gattatagaa aaaggtgatt
    37861 ttgtacttat agactatggt gctatgtata atggctatat atctgataca actagaactt
    37921 ttatagttgg aggagcttct gaaaagcaat tagaaatata taatttagtt aaggaagctc
    37981 aaaatgtagg ggttgagaac atgaaagctg gtgtacatgc gacaattcca gatgctgaga
    38041 taagaaaagt tgtcaaaaaa tatgaagact attactatca aggtatagga catggagtcg
    38101 gtagagatgt tcatgaagaa cctttcattg gtaattatgg tgataaaatc atagaagagg
    38161 gatgtataat aacaatggaa ccaggtattt atttccctgg atggggtgga gttagaatag
    38221 aagatactgt tttgattact aaaaatggtc cagaaagact tactaaattc cctaaagatt
    38281 tgatgatttt agacaaataa tttaatttat atatattttt ttacataaaa tataacataa
    38341 tctcactatt aagctctatt tacatattat agtaaagagg gcttaatttt attgtgagag
    38401 gtgatagtat atatgtataa aaaacataaa tttataaaaa atataaaaaa agcctggtat
    38461 aaaatcaaat taattgatag atgccttata attgtaatgg caatattaat gtttcaatct
    38521 atatataact tatttgttaa tgaagtaaat tcacaagata ctacaactat tgatgtagta
    38581 gtaagaacca cttcagcagc tatatttggg tattttttaa gtgctaattt tataaagaga
    38641 cctttaagga agtatacaaa tactgatgta tcttcaaatc tttttataga taataattta
    38701 tccccaaaag gtcaagagtc taatcaaaat aatataatga atgtcaaaaa taccattgga
    38761 tttacttcag aagctgataa ttatgaaaag aaaataccta ttaataataa tgaaggtttt
    38821 gaagaaggag aaactagtga attgcaaatt attatagcta ctgtaatttg catagtagca
    38881 ttaacagtac tttttgttgt aagaaatttt acaactacaa catcagcttc tttaggtact
    38941 atttcacaga tgagagattt tgtctcagga tgcgtgggtt ttttgctagg ttgtccaagt
    39001 aaacctgatt aaaaaagtat tatcaataaa aggtatttta aaaagtatca gtataataat
    39061 atttagattt aaatgtatac aattataatt ctgtaacatt ctatattgta tttttatact
    39121 gatactctaa actatactat ttattaaaat attttaacat tgtatttaaa caaaatatta
    39181 tatctgtata atataaattt agtttatatg aatagatgcc atatactcaa taaaaagttt
    39241 catactatga gatatgtatt gatttcttct ccaaagaacc ccaatatttg cagtaattgc
    39301 acgttctaat ggtattgaac agagattttc tgtattttga aatatttctt tatacaatat
    39361 tgttgaagcc aagtcatttt ctagtaaata acgtatggta gaaagttgat ttgagtgtaa
    39421 aattatattt ggcttagctt tttcaagtct ttttgtaata atcttatagt gataagaacc
    39481 ttcagataag attacaaaag gtacattttt taacatataa ttcgatataa taggatgttt
    39541 tgccaattca tttttgggat ttgtaactaa gtataattct gttttataga ttgtctttga
    39601 atctatatca tcataaatat cactgtcaat tataccaata caaaaatcaa gtgtagattc
    39661 ttcaatcatt ttagctccaa caaatgttgg aacttcaaaa attttaagct ctatatctgg
    39721 ataaattaca ttaaacctag gaagtatttt tgaaagcaaa aacgtaccta aaactgtagg
    39781 aatacctatt ttgagagaag cttttctctt ttttcctaaa tcaagtgcgt cacattgaaa
    39841 atcctcaaat tgttttataa aatgttttga cttgtctaaa aaaatttttc cttcaggcgt
    39901 taactctatt ttattaccta ttctgtaaaa aagttcaaag ccaagttctt tttctaattc
    39961 tttaattgct aatgttatgg atggttgaga aatatgaata ctttccgcag ccttagtcat
    40021 gtttttatat ttacagacag tttgaaaata gtataaatag tttatattca tttggtatta
    40081 gttcctttca tttacatatt agtgacaagt atataattaa caatagcata agttttgtat
    40141 attgttgtaa aaaaatagaa tagttttaaa ctatcatagc ataagttttt gatattttca
    40201 atctattaag aatagtgcta ttattaaatt aacaaaagag aatagctggt atggtttaga
    40261 gtaaatcaat ctatattaca gttatatcaa cgaatatcaa aaaattatct attaaaaaat
    40321 aaaaattatt tttacaatta aattgttaat taaatcataa agaaaaaagt gatattaaca
    40381 agataaaatt tttttaatca gaaatgttaa tttaataaca aaatagagta ttggggagaa
    40441 aggagaaata tattttttaa taatatttgt taatttaata acgaaaaatg aaagggagtg
    40501 atttagtgaa gtttgaattg atggattttt taatgaatcc ttttgtcctt atgtttgctg
    40561 cagtaattac aggaatttta tttggaaaaa ttaaatttgg gaagtttaat tttggggtat
    40621 caggagcatt atttacagga ttatttattg gatggcttgc atatagcttg gggaatctaa
    40681 taatagcaaa aggggaaaca gcagcaggat ataaggcagc aactgtaatg atggggaatg
    40741 gaattatttc ttctgacttc tttgatttct ttttaataat atttgttgca gcagttggtc
    40801 tattggcagc aaaagatatg aaagctgtat taaagaagta tggagcaaga tttgtaattt
    40861 taggagttct tataactttt ataggtggct ctatgactta tgcaatgact ttactatcaa
    40921 gtgataaagg aagtagtgct tatgaagttt ctggcgttta cacaggagca ttaacaagtt
    40981 cgcctggatt agctgctgct ttagaaacag ctggtaaaca tgcagaagat gtatcaaaag
    41041 aatttgaaaa agcatcaata aaagataaaa aagaaatttt aaaagtagtc gacccagaag
    41101 gcaaattgga tgtgaataca actacatcac tgactcaaga gcaaatagat aagtatattg
    41161 catatgctga agctggtgtt ggtataggtc atgcagtcgc atatcctttt ggagttttaa
    41221 tagtaatctt aggtgtaaat ttccttccaa aattatttag gatggaccta aaagaagaaa
    41281 gaagaaaata tgagaaggaa atgaaggaag caagagatag tgtttctggt aaaacgatac
    41341 cagaagttcc atttaatata ataacatttt tcttgacatg tttagctgga tacttagttg
    41401 gaggaataca tgtatttatg ggaccactag ggtacttcac actaggtgca acaggaggtt
    41461 cactaattgt atctctagtt cttggatata taggtaagat aggggtagta aatttcagaa
    41521 tggaagaaaa ggttttaaat attttaaaac aaatagggct agtatttttc ttagcaatag
    41581 ttggattaag atatggtgga aaagtagttg attcaattat gacatctggt atgcacctag
    41641 ccttagtagc aatagcagta ggtgttacag ctatgatgat aggattctta gttggcaaat
    41701 acgtatttaa attaaattgg attttattat ctggagctgt ctgtggtggt atgacatcaa
    41761 caccaggtct tggagctgct gttgatgctc ttgatagtga tgacccagca gcaggttatg
    41821 gcgctacgta tccatttgca ttattaacta aagttatatt ggttatagtt ttacacaaac
    41881 ttccaatgta aataagattt tatatggggg gatatttatg aataatgaag gagcatttga
    41941 agaattaagt ggaagattaa gaaataaaga attgtgtgat ttaataatga atgcagatga
    42001 agctgctaaa attatagagg atggcatggt tattggagtt agtggattta caccttcagg
    42061 ctatccaaaa gcagtaccat tagcagtttc agaaagagct aaaagtggag aaaagataaa
    42121 attaaccgta tattcaggag catctttagg tccagaagtt gatggagcat ggtcagaagc
    42181 aggtataatt gaaagaagac ttccttatca aactaattca atacttagaa ataatattaa
    42241 taaaggtgtt gttgattata tagatatgca cctaagtcat tctactcaat ttttaaatta
    42301 tggaactata cctaaagtag atgtagctat agtagaagca cttgcaataa ctgaagaagg
    42361 gaatattata ccaactagtg ggataggtaa ttctccatct tttataaaaa gtgcagataa
    42421 agtaatagta gagataaatc ttgcaaaacc aatggaaatg gaaggtatgg cagatatata
    42481 tattacggaa aatccaccaa atagaaaacc aatagaaata aatcatccta aggatagaat
    42541 tggaacaaca tatatacctt gtggtttgga taaaattgca ggagtagtaa ttaccaatat
    42601 gcaagataag acccgtccat tgggggttgt agatgaagct tcgaaaaaaa tatcaaataa
    42661 tattattgct tttttaagag aagaagtaaa ggcaggcaga ttatcaaaga atttacttcc
    42721 tcttcaatct ggtgtaggaa gtgtagcaaa tgcagtatta tatggattat gtgaatctga
    42781 atttgaaaat ttaacttgtt atacagaggt tgtacaggac tctatgttgg atttaataag
    42841 aatgggtaaa gttacaatgg catctactac atctgtaagt ccatctccag aaggattaat
    42901 aaaatttgaa aaagatattg atttttttaa ggataagatt atattaagac ctcaagaaat
    42961 tagtaacaat ccagaaatag ccagaagaat tggtgtaatt gcaatgaaca ctgcaattga
    43021 agttgatatt tatggaaatg taaattctac tcacataatg gggtcaaaga tgatgaatgg
    43081 aattggtggt tctggtgatt ttgctagaaa tggagcaata actatattta gtactgagtc
    43141 aattgcaaaa aatggtgata tatcatcaat agtacctatg gtatctcacg tagaccacac
    43201 tgaacatgat gtaatggtaa ttgtaacaga acaaggatat gctgatttaa gaggtcttgc
    43261 acctagagaa agagctataa aaataataga aaattgtgcc catccagatt ataaagaaca
    43321 acttagggat tatttaaata gagcttgtaa aagtggagca aaacaaacac ctcacatatt
    43381 ggacgaagct ttatcatggc atagtaaatt tatgagtaca ggtacaatga aaaaggctga
    43441 aacttttaaa agtgcattat aaaatattta ataaattgga ggtagatttt aaatggaaaa
    43501 agcagtggaa aactttgaag atttaagtaa ggaatacatt aatggttata ttgagagagc
    43561 aagaaaagct caaagagagt ttgaatgtta tactcaagaa caagtagata aaatagtaaa
    43621 aatagttgga aaagtagtgt attataatgc tgaatattta gctaaacttg ctgtagaaga
    43681 gactggaatg ggagtatatg aagataaggt agctaaaaat aaaagcaaag caaaagttat
    43741 atataacaat ttaaaagata aaaaatcagt tggtataatt gatatagata gagaaactgg
    43801 aataacaaag gtagctaaac cagttggagt agtagcggca ataactcctt gtacaaatcc
    43861 aatcgttaca cctatgagca atgcaatgtt tgcacttaag ggaagaaatg caattattat
    43921 cacacctcat cataaagcaa ttggatgtag tacaaaaact gtagagatga ttaatgaaga
    43981 attagaaaaa ataggagctc cagaaaattt aatacaaata cttgaccaac aatcaagaga
    44041 aaatacaaga aatttaattt catcagcaga tgtagtaatt gccactggag gaatgggaat
    44101 ggtaaaagct gcgtactcaa gtggaaaacc agcacttggt gtaggggcag gaaatgttca
    44161 atgtatcata gatagagatg tagacattaa agaagtagta cctaaaatca tagcaggaag
    44221 aatttttgat aatggtatca tttgttcagg tgaacagtca gtaatagtag cagaagaaat
    44281 gtttgataaa ataatggatg aatttaaaaa taacaaaggt tttatagtta gagataaagt
    44341 acaaaaagaa gctttcagaa atgctatgtt tgtaaataaa tctatgaata aagatgcagt
    44401 aggtcaatca gttcatacaa ttgcaaaaat tgcaggtgtt gagataccag aagatacaaa
    44461 aataatagta atagaagctg atggaccagg agaagaggat attattgcaa aagaaaaaat
    44521 gtgtccagtt atatctgcat acaagtataa aagttttgaa gaaggtgtag ctattgcaaa
    44581 agcaaattta aatgtagaag gaaaaggaca tagtgtatct atacattcaa atacagttaa
    44641 aaatatagaa tatgcaggag aaaatataga agtatcaaga tttgttatta atcaatgttg
    44701 tgctactagt gctggaggaa gtttctttaa tggtcttgca cctactaata ctctaggttg
    44761 tggttcttgg ggaaataaca gcatctctga aaatcttgat tataagcatc taattaatat
    44821 ttctagaatt gcttattata tgccagaaaa tgaagtccct acagatgaag aactttgggg
    44881 ataaaattta gacaaaaaat attcattcta aattaaagta ggaatatact ttaatttaga
    44941 atgaatttaa gacataaatt aaatttaaat tagtttattg gactaaattt aaaatattaa
    45001 gagacatata gaagaaggaa gtattataat ttaaaataaa taaaaaaatc tataaaagag
    45061 aaggagtggt tttattatgg cattaatgac aggagctcaa tatattgaaa gtttgagaaa
    45121 attaaatact aaggtttata tgtttggtga agaggttaag aattgggttg accatcctat
    45181 gattagacca tctataaatt gtgtagcagc aacttatgac ttagctcatg acccagaata
    45241 tgcagattta atgacagtta cttcaaatat cacaggtgaa aaaataaata gatttggtca
    45301 tcttcatcaa agtgttgatg atttgataaa aaaagtaaaa atgcaaagac tatgtggtca
    45361 aaaaacagct tcttgtttcc aaagatgtgt tggtatggat gcgtttaatg cagtgtattc
    45421 aacaacattt gagtgtgata aagcccatgg aactaattat catgataatt ttgtaaaata
    45481 cttaacttat atacaagaaa acgatttagt agttgatgga gctatgactg accctaaggg
    45541 agatagaagt ctatcaccta gtgctcaacc tgacccagat atgttccttc atatagtaga
    45601 aagaagagaa gatgggataa ttgtaagagg agcaaaggca catcaaactg gttcaataaa
    45661 ttcacatgag caccttataa tgcctactat aagtatgact gaagcagata aagactacgc
    45721 tgtatcattt gcagttcctt cagatgctga aggagtgttt atgatttatg gaagacaatc
    45781 ttgtgacact agaaaattag aagaaggtgc agatgttgat ttaggaaata aggagtttgg
    45841 aggtcaagaa gctttagtag tatttgataa tgtatttgtt ccaaatgata gaattttctt
    45901 atgtggagaa tgggatttct ctggtatgtt agtagagaga tttgcaggat accatagaca
    45961 aagttatggt ggatgtaaag taggagtagg tgatgtaata ataggagcag cagctttagc
    46021 agctgattat aatggagcaa acaaagcttc acatataaaa gataaactaa tagaaatgac
    46081 tcaccttaat gaaagcttat attgctgtgg tatagcatgt tcatcagaag gtcacaaaac
    46141 agaagctgga aactatcaaa tagatttact tcttgcaaat gtgtgtaaac aaaatgtaac
    46201 tagatttcct tatgaaatag taagacttgc agaagatata gctggagggc ttatggttac
    46261 tatgccttca gaaaaagatt ttaaatcaga ccttaaagtt ggaacaagtg gaatgactat
    46321 tggcgaagta tgtaacaagt attttaaagc ttcttcagtg gcttcaacag aagaaagaat
    46381 gagaatatta agattcttag aaaatatatg ccttggttca tctgctgttg gttatagaac
    46441 tgagtcaatg catggggcag gttcaccaca agctcaaaga ataatgattt caagacaagg
    46501 aaacataaat caaaagaaag aattagctaa gaaaattgcg ggaataaaaa aagaggaagc
    46561 tttaaattta taaaaagttg atatataaag ggaaattata tattaatgtg tgttaattaa
    46621 tgaaaaaata tgtagtataa gagatagtta taaaattatg gtggttttta gctaagtaaa
    46681 gaaattttta ataaggagaa tttacacatg acctgtggag ttagattttg tgggggctgt
    46741 aaccctagat acgaaagagg gaaggctctt acaataatca aagagcactt caatggtagg
    46801 gtagatttta gtatagcaca agagaacgtc gagtatgatt tgctacttat aattggagga
    46861 tgcgcaactc tatgcgcatc ctatcaacaa tataaagtga aaaatgaatg tgttaagatg
    46921 tgggatttta gccatataga aagagttata aatgtgattg aatcagcaat ttgtgaaact
    46981 taaaaaagct aaatgggggg atttcattga gttggcaaga attgtatcaa agtaaattat
    47041 gtagtgctac agaagctgta aaacagatta aaaacggtga tactgtagta tttgcacatt
    47101 gtgtaggtga acctcctgca ctagtagagg ctatgattga aaatgctgaa caatataaag
    47161 atgttgagat taaacatatg gtaagcttgg gtagtggtgg atatactgct aaaggaatgg
    47221 aagctcattt tagagtaaat ccaatgtttg taagtggaaa tgtaagaaag gcaattgaaa
    47281 atggagatgg tgattttaca cctgcattct tccatgaagt accaaagttg cttagagaaa
    47341 aaagattaaa atgtgatgtt gttttagcac aggtaactcc accagatgaa catgggtatt
    47401 gtagcttagg aacatcagtt gattatactt atgaagccat taaaactgct agaacagtaa
    47461 ttgttcaagt gaatgaccag tttcctagaa cctatggtga ggtagtccat gtatcagagt
    47521 ttgactatat agtggaaaaa tcacaacctt tatttgaact acaacctgca aagattggag
    47581 aagtagaaga agcaattggt aaaaattgtg cttcattaat tgaagatggt tctactttac
    47641 agttagggat tggtgggata ccagatgcag ttatgttatt tttaactgat aaaaaggatt
    47701 tagggattca ttcagaaatg atttcagatg ggactttggc cctttatgaa aaaggtgtta
    47761 taaatggtaa atataaaaat tttgataaag aaaaaatgac agttaccttc ttaatgggta
    47821 ctaaaaaatt atatgacttt gcaaataata accctgcagt agaggtaaaa ccagtagact
    47881 atgtgaatca tccagcaatt ataatgaaac aacataagat ggtttctata aattctgcta
    47941 tacaagttga tttaatggga caggtagttg cagaggctat gggacttaga caatttagtg
    48001 gtgttggagg tcaagttgac tttatacgtg gagtgtctat gggtgaagat ggaaaggcaa
    48061 ttatagctat gccttcaata actacaaaaa aagatggtac agtaatttca aaaatcgtat
    48121 ctattgtgga tgaaggtgca cctataacga catcaagaaa tgatgttgac tatattgtaa
    48181 cagaatacgg tatagcagaa ttaaaaggaa aatctcttag agaaagagca agaaatttaa
    48241 taaatatagc tcatccaagt gtaagagaaa gtctagcagt agaatttgaa aagagattta
    48301 aggagaaata ttaataatag aggggtgtta ataatggaag cacttagagt agttccaaag
    48361 atattttact ttgatacatt taaagaattt aatgaagaat ttaaaatagg aaaaaatgat
    48421 ttagtaatta ctaatgagtt tatatatgag ccatatatga aacctcttgg aatagataca
    48481 aatttaattt ttcaagaaaa gtttggaaca ggtgaaccat ctgatgaaat gatagactcc
    48541 atgacaaagg aaatgaagaa atacaatttt gatagaatta ttgcatttgg aggaggaaca
    48601 atagttgata tatgtaagat attagcactt gatgttccag aaaagtctat tgatttattt
    48661 gaaggagaag tatcacctaa aaaagtaaaa gagttagtag tagttcctac aacatgtggt
    48721 acaggtagtg aagtaacaaa tgtagctatt gcagagttaa aatctaaaca tactaaaaag
    48781 gggcttgcag tagaagaaac atatgcagac tatgcagtgt taattccaga gacagtaaaa
    48841 gggttacctt acaaattctt tgttacaagt tcggtagatg ctcttataca tgctattgag
    48901 tcttatcttt ctccaaaagc aagtccattt acagagatgt attctctaca agcaataaaa
    48961 atgattatgg atgggtataa gaagatagtt gataaaggag aagaagagag atttaatcat
    49021 cttagagact ttgtacttgc atcaaattat gcaggaattg cttttggaaa tgctggttgt
    49081 gcagcagttc atgcactatc ttattcaata ggtggagctt tccatgttgc acatggtgaa
    49141 gcaaattatc agttctttac tgaagtattt aagatgtatt ctagaaaaaa acctaatggg
    49201 aaaattataa aatgtacaaa aattttagca gatgcccttg aatgtgaccc taactgtgat
    49261 gtttatggag aattagaaaa gtttttaaat aaattaatag ctaaaaaagc tttgagagaa
    49321 tatggaatgg tggaaagtca gatagatgaa tttacagata gtacaatagc aaatcaacaa
    49381 cgactacttg ctaataatta tgtagagctt agtagagagg aaataagaga gatatttgca
    49441 aatctttatt aatacaattt tttatttata tgtatagtca acataaacaa tgctaattat
    49501 atttatgtac aaataaaaat aagggaactt aaattttaat taaagttaag ttctcttatt
    49561 tttatatgga gttaaatata agtatatttt taatttaaaa tatgcttata tttaagtcta
    49621 ttactttatt tgaaacaatt tgtagaattt caaattcctt gacagtaata agtaagtaat
    49681 tgtataatca aactgcaata tattaataat attagtgaaa atgaaaggat tataaaatat
    49741 gtataaatca gaaaattttt caaaaaaatc tgtattaata aattcatttt taagtaaatt
    49801 tttaaaaata attggatggg gtttggtaag cttatttaca ttatcagcct tagtactttc
    49861 aactgtttcc attataattg gatgggctgt aatacttcct attcaagtat gtgcagtatt
    49921 tttacttata ggaggagttt gtatttttaa aggtaagaga ttaggagacc aaatttctcg
    49981 ttataaaaaa tattgtacaa ttataaacaa tagaaatatt attccagtag aacttattgc
    50041 agaaaacaca tctaaatcat taaattttat aataaaagat gtacaaaaga tgatagacaa
    50101 aaactacttt ataaatactt atattgataa aagaaataat cagattgtgt taacaagtga
    50161 agattttgtt ccaccaagat atgaagatgt agcatatgaa gtcaaagaag aagaaaagac
    50221 tgatgaatcc aatgaagttg atacaattat aaataaagga atgaattatc taacacaaat
    50281 aaaagaagct aataaaaata ttaaatcaga atcaatgggc aataaaattg tacaagtaga
    50341 agatgttaca agtaaaatat ttgatgttgt aaaacatgac ccatcaaaac ttactcagat
    50401 acaaaaattt atggattatt atcttccaac cactttaaaa ctattaaatt catatcatac
    50461 attggaagaa caaggaattg atagagaaaa tataacaaca actatggaaa gtattgaaaa
    50521 tacaatgaat acgattgtag tagcttttga aaatcaattg gactatctgt ttgaagatga
    50581 agccattgat atttctacag atattactgt tttagagaat atgttagtcc aagaaggatt
    50641 aacgagtggt aaattttaac ttcaatagaa aaaatattct gttattttta aacaattaat
    50701 tagtcataaa gtataaaatg tgtattatat aagttaatca gtactttatg aaggaagagg
    50761 agagaaagat acaatgaata atttagatga tataccagtt atgccaactt tgacacttga
    50821 cccatttgga gaagaatcaa acactaatga tatagataat tctgacttat taatgaaaaa
    50881 ggatgaaaaa gacccagaag aagaaaaact gtctgaatct gaacgcaaga tggtaaagga
    50941 atttgcggaa aaaatagata ttacaaatac aaatatgatt ttgcaatatg gagctggtgc
    51001 tcaaaaaaag gtagcaagtt tttctgaaac agctttaaag agtgtaaaga caagagattt
    51061 aggagaagtt ggagatatgc ttacaaatct agttactgat ttaaaagcat tttcagcaga
    51121 tgaaaaagaa caaagtggtt ttttaggaat ttttaaaaag gcaaataata aaatttcaaa
    51181 tttaaaaact aagtatgata gtgcagaggt aaatgtggat aaagtaagca aagagcttca
    51241 aaagcatcag gttaaaatat tgaaagatat agcaatgctt gataagatgt atgaattgaa
    51301 cttagcttac tcaaaagagc ttactatgta tattatagca ggaaaacaaa aattaaagga
    51361 tatgaaagaa tatgagatgc caaagttgag agaaaaagct cgtttatctg gttcaacaga
    51421 agatgctcaa tctgtaaatg atatggtttc actttgtgac agatttgaaa agaagattca
    51481 tgacttagaa ttaactagaa tggtatcgct tcaaatggct cctcagataa gattggttca
    51541 aaatagtaat aatctgatgg cagaaaagat tcaatcaact atagtaaata ccattccatt
    51601 atggaaaaat caaatagtgc ttgctcttgg aatttctcac ttaaatcaag ctatgaaagc
    51661 acagcgtgaa gttagtaata tgacaaatga gcttttatta aagaatgcag aaactttgaa
    51721 gatgggaaca atagaaacag caaaagaatc agaaagagga attgtagata tagacacaat
    51781 taagaaaacg aatcaatctc taatatctac tattgatgag gtagttaaaa tacaacatga
    51841 tggtagaata aagagacaag aagcagaagt tgaattatca aaaatagaaa atgaacttaa
    51901 aagtaagctt cttgaattta cagttaaata aaaattatac taatataaaa agagactgtc
    51961 tcaaaataga gataagtctc tttttcattg tttaaatgat taaagtatat ttatattata
    52021 aatttctatt atcttttaac ataacatcat gagctccacc ttcaactata ctagtagaag
    52081 atacaagtgt aagtttagca tttttttgaa gttcaggaat gctaagtgct ccacagttac
    52141 acatagtaga acgaacttta cttaaagaaa gagttacatt gtctttcaaa gaaccagcat
    52201 aaggcacata agagtcaaca ccttcttcaa aagaaagttt cttatctcca ccaaggtcat
    52261 atctttgcca gtttctagca cgagcagaac cttctcccca atactctttc atataactac
    52321 catttatatt aactttgtta gttggactct catcaaaacg agagaagtat ctaccaagca
    52381 ttataaaatc tgcacccata gctaaagcta aagttatatg atggtcataa acgataccac
    52441 catcagagca aattggaata taaatacctg ttttttcaaa gtattcgtct ctagcttgag
    52501 caacttcaat tatagaagta gcttgtccac gtcctatacc tttttgttca cgagttatac
    52561 atatagaacc accaccaata ccaactttaa cgaagtcagc accagcttca gctaggtaaa
    52621 ggaatccttc gcggtcaact acatttccag ctcctacctt aactgtatca ccatattttt
    52681 cacgaatgaa atcaagtgtt attttttgcc actcagaaaa accttctgaa gaatctatac
    52741 ataaaacatc agcaccagct tcaactaaag caggaacacg ttcagcaaag tctctagtat
    52801 taataccagc tccaactaca tatctcttag aagaatctaa aagttcaagt ggattttctt
    52861 tatgagaaga gtaatcttta cggaatacca tatacataag attaccatta tcatcaagta
    52921 caggaagaga attaagtttg tggtcccata taatgttgtt tgcttccttt aaagtaacat
    52981 ctttgttagc ataaactata gatgacatag gagtcataaa ttcactaact ttagtatcta
    53041 aatccatacg acttatacga taatcacgac ttgcaacaat acccaataat ttaccagttg
    53101 aagttccatc ttcagtaaca gctactgtag aatgtccagt tttttctttt aagtcaagta
    53161 tgtcttttaa tgtgttatca ggtttaatat ttgaatcact tacaacaaaa ccagctttgt
    53221 gggatttaac acgtgctacc atagcagctt cattttcaat agtttgagaa ccgtatataa
    53281 atgaaatacc accttcttta gcaagtgcaa tagccatttt gtcatcagaa acagattgca
    53341 taatagcaga agtcaaaggt atattcatat aaatatctgc ttcctcgccc tttttaaatt
    53401 tagtaacagg tgtttttaaa cttacattag caggtataca atcagtagaa gagaaacctg
    53461 gtacaagaag gtattcactg aaagtatgtg atggtgtatc aaaataaaac gccatttttt
    53521 aatcactcct tatattttta tactttttat aattgatagt attaattata acttattctt
    53581 taattttatt aaagtatttt gtcaaatatt gagatttttg aaaaatttaa actattttaa
    53641 ttaaatttgt ttaatatata ttgccctata gggtaaagt
//
LOCUS       DAEMIK010000006        50425 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEMIK010000006 DAEMIK010000000
VERSION     DAEMIK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.006"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(66..911)
                     /locus_tag="KPK87_000302"
     CDS             complement(66..911)
                     /locus_tag="KPK87_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536503.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(941..1312)
                     /locus_tag="KPK87_000303"
     CDS             complement(941..1312)
                     /locus_tag="KPK87_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2536504.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(1465..1725)
                     /locus_tag="KPK87_000304"
     CDS             complement(1465..1725)
                     /locus_tag="KPK87_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG2536505.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(1914..2858)
                     /locus_tag="KPK87_000305"
     CDS             complement(1914..2858)
                     /locus_tag="KPK87_000305"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG2536506.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(2879..3733)
                     /locus_tag="KPK87_000306"
     CDS             complement(2879..3733)
                     /locus_tag="KPK87_000306"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG2536507.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVITGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(3748..5109)
                     /gene="accC"
                     /locus_tag="KPK87_000307"
     CDS             complement(3748..5109)
                     /gene="accC"
                     /locus_tag="KPK87_000307"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG2536508.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(5125..5577)
                     /gene="accB"
                     /locus_tag="KPK87_000308"
     CDS             complement(5125..5577)
                     /gene="accB"
                     /locus_tag="KPK87_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG2536509.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(5847..6293)
                     /locus_tag="KPK87_000309"
     CDS             complement(5847..6293)
                     /locus_tag="KPK87_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536510.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYNNVLIAKLKYILCKNKENDTNLKESEV"
     gene            6568..6846
                     /locus_tag="KPK87_000310"
     CDS             6568..6846
                     /locus_tag="KPK87_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536511.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(6975..7721)
                     /locus_tag="KPK87_000311"
     CDS             complement(6975..7721)
                     /locus_tag="KPK87_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536512.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(7850..8494)
                     /gene="trmB"
                     /locus_tag="KPK87_000312"
     CDS             complement(7850..8494)
                     /gene="trmB"
                     /locus_tag="KPK87_000312"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG2536513.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(8632..8952)
                     /locus_tag="KPK87_000313"
     CDS             complement(8632..8952)
                     /locus_tag="KPK87_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG2536514.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(9405..9653)
                     /locus_tag="KPK87_000314"
     CDS             complement(9405..9653)
                     /locus_tag="KPK87_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2536515.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(9684..10145)
                     /locus_tag="KPK87_000315"
     CDS             complement(9684..10145)
                     /locus_tag="KPK87_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG2536516.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLALGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(10183..11016)
                     /locus_tag="KPK87_000316"
     CDS             complement(10183..11016)
                     /locus_tag="KPK87_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2536517.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(11290..11976)
                     /locus_tag="KPK87_000317"
     CDS             complement(11290..11976)
                     /locus_tag="KPK87_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536518.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVKENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(11991..14468)
                     /locus_tag="KPK87_000318"
     CDS             complement(11991..14468)
                     /locus_tag="KPK87_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2536519.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMEKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(14687..16090)
                     /locus_tag="KPK87_000319"
     CDS             complement(14687..16090)
                     /locus_tag="KPK87_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2536520.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            16583..17443
                     /locus_tag="KPK87_000320"
     CDS             16583..17443
                     /locus_tag="KPK87_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2536521.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(17682..18239)
                     /locus_tag="KPK87_000321"
     CDS             complement(17682..18239)
                     /locus_tag="KPK87_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2536522.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(18429..19316)
                     /locus_tag="KPK87_000322"
     CDS             complement(18429..19316)
                     /locus_tag="KPK87_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2536523.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     ATRFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            19435..20634
                     /locus_tag="KPK87_000323"
     CDS             19435..20634
                     /locus_tag="KPK87_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG2536524.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(20828..23383)
                     /locus_tag="KPK87_000324"
     CDS             complement(20828..23383)
                     /locus_tag="KPK87_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2536525.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLDLNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(23380..24066)
                     /locus_tag="KPK87_000325"
     CDS             complement(23380..24066)
                     /locus_tag="KPK87_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536526.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     IVVNTGGEVL"
     gene            complement(24143..25051)
                     /locus_tag="KPK87_000326"
     CDS             complement(24143..25051)
                     /locus_tag="KPK87_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2536527.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(25053..25724)
                     /locus_tag="KPK87_000327"
     CDS             complement(25053..25724)
                     /locus_tag="KPK87_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536528.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            25986..27050
                     /locus_tag="KPK87_000328"
     CDS             25986..27050
                     /locus_tag="KPK87_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG2536529.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            27208..27894
                     /locus_tag="KPK87_000329"
     CDS             27208..27894
                     /locus_tag="KPK87_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536530.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLIIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            27884..28915
                     /locus_tag="KPK87_000330"
     CDS             27884..28915
                     /locus_tag="KPK87_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2536531.1"
                     /translation="MNFKKFSFNSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFITLIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMIKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            28997..29662
                     /locus_tag="KPK87_000331"
     CDS             28997..29662
                     /locus_tag="KPK87_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536532.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            29675..32092
                     /locus_tag="KPK87_000332"
     CDS             29675..32092
                     /locus_tag="KPK87_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2536533.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(32273..33053)
                     /locus_tag="KPK87_000333"
                     /pseudo
     CDS             complement(32273..33053)
                     /locus_tag="KPK87_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(33086..33673)
                     /locus_tag="KPK87_000334"
     CDS             complement(33086..33673)
                     /locus_tag="KPK87_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2536534.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(33850..35241)
                     /locus_tag="KPK87_000335"
     CDS             complement(33850..35241)
                     /locus_tag="KPK87_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG2536535.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(35431..36729)
                     /locus_tag="KPK87_000336"
     CDS             complement(35431..36729)
                     /locus_tag="KPK87_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG2536536.1"
                     /translation="MKVNVYNVESFVDERLKIMVSNLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALKIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(37088..37963)
                     /locus_tag="KPK87_000337"
     CDS             complement(37088..37963)
                     /locus_tag="KPK87_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG2536537.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(38156..38842)
                     /locus_tag="KPK87_000338"
     CDS             complement(38156..38842)
                     /locus_tag="KPK87_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG2536538.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(38891..39424)
                     /locus_tag="KPK87_000339"
     CDS             complement(38891..39424)
                     /locus_tag="KPK87_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG2536539.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(39468..40100)
                     /locus_tag="KPK87_000340"
     CDS             complement(39468..40100)
                     /locus_tag="KPK87_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG2536540.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(40190..41269)
                     /locus_tag="KPK87_000341"
     CDS             complement(40190..41269)
                     /locus_tag="KPK87_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG2536541.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(41402..42748)
                     /gene="pepV"
                     /locus_tag="KPK87_000342"
     CDS             complement(41402..42748)
                     /gene="pepV"
                     /locus_tag="KPK87_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG2536542.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(42817..44097)
                     /locus_tag="KPK87_000343"
     CDS             complement(42817..44097)
                     /locus_tag="KPK87_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG2536543.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(44224..44484)
                     /gene="hfq"
                     /locus_tag="KPK87_000344"
     CDS             complement(44224..44484)
                     /gene="hfq"
                     /locus_tag="KPK87_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG2536544.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(44598..45533)
                     /gene="miaA"
                     /locus_tag="KPK87_000345"
     CDS             complement(44598..45533)
                     /gene="miaA"
                     /locus_tag="KPK87_000345"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG2536545.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(45571..47538)
                     /gene="mutL"
                     /locus_tag="KPK87_000346"
     CDS             complement(45571..47538)
                     /gene="mutL"
                     /locus_tag="KPK87_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG2536546.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVITLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(47549..50392)
                     /gene="mutS"
                     /locus_tag="KPK87_000347"
     CDS             complement(47549..50392)
                     /gene="mutS"
                     /locus_tag="KPK87_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG2536547.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KKYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSLNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
BASE COUNT        17191 a         7927 c         5682 g        19625 t
ORIGIN      
        1 tcttttttat ataatatttt tatatgtttc taacgaaacc aaatattagt aaaatatgta
       61 tttatttaac taaagttttt aaatgggctg actaagctta aaatatagga tattatgaga
      121 gttactattg aaaaaataaa ccctattgcc ataaaaaaac ttataaatat gttatactta
      181 actattaaac tggatatatt caagttatca aatccaaata agtatgtaat tgaacttatt
      241 acaaaaataa ctataactat aaagctcaga ccatttaagc tattttttaa atcagcacta
      301 ctaagtgaca tatgtgtaga tatagaatac attaaaaaca taaatataaa atacttcaat
      361 gaatatacac taggtgttaa caacattatt cttataaaat tggaaatatc acctaaaata
      421 atgtgtataa acccatttat atttaaatta ttaatcatac ttacatataa ctttaaatct
      481 atattttgag tcactttcat ataactatct ggcagtaata tcttaaaaat taaaataatc
      541 attattgttc cacctataat aggtgcaatt cctataaaga aatttcctat actttgataa
      601 agattatctt ttttatagct atgacttaca tatcctagta taccatcatt tttactttct
      661 attggtctga aaaacttaac tgaatttatt ttatgcatga aaattaaaca cattatcaaa
      721 tgactcaatt catgaacaaa cgttcctata gctccagtta caatgattcc agtttttcca
      781 aaagatgaaa atataaattt attatttaga ttttctgtaa tgctaaaaat caatccaaat
      841 acaataaata ttcctatgaa acaaaaaata tttattacac ttatttttaa aatttcgatt
      901 atataaatca taagttaagt cctcatgtaa aagtatttaa ctactctact tcatatccat
      961 tacagcttaa aattttacaa gcattttcta tatctttttc ttttactaag atatagtctg
     1021 tatcataagt agaaattgca aatatgctta ttttgttgtc tgctaacaaa ctacttaact
     1081 tagataaaat tcctacaagt gaaaagtcta aaggacctag tatcttaagt attctccact
     1141 ccttttctga cttaacatca gaaggaatat tattatttaa acatacaaca cttaattcat
     1201 catcagttct agttatagaa taaaacttct ctgtatctat ccatgttggt attttactgt
     1261 cattatttaa tctacatact gcatactctt ctaccaataa ttttaatttc ataatgtata
     1321 tttcctccaa tcttatttgt ttacttaaga ttatacatta aaagttacat aaaataaaac
     1381 atataaagta aaaaaggttc actatagtgt cctatttcta tagccaacct ttaaaactta
     1441 attaaattct aataaagaga tatattatct aggttcaatt ataagtttaa tggctgtttt
     1501 cttatcacct tcgatttcta tatcagtaaa tgcgggaatg caaactaaat ccattccact
     1561 aggtgcaaca aaacctcttg ctattgcaat tgattttatc gcttgattta gagctccagc
     1621 tcctatagct tgtatttctg caacgcctcg ttctcttagt actccagcca aggctccagc
     1681 tacagaatta ggattagatt ttgatgaaac ttttagtacc tccatttcaa catctccttg
     1741 ttaatttgtt aaagtatcca ttacctaaag tactaaatca taattgccat ttactaatta
     1801 caatcatcat ttaaagtata cttttataaa aatatacttc tataatcgtt ttaagaaaac
     1861 ctttattttt tttaaccatt ttattatgaa aaaactacaa atttctttat attctataag
     1921 cattttccaa tatttctaaa ttttttatat ctttgagcta taatttcctc tttatccata
     1981 tctttgtaat gatttaatct ttctaaaata taagctttta tttcttttga aacagcttct
     2041 acatcttttt gtgctcctcc aagaggttct tttataatct tatctattat ttttaaatca
     2101 tataaatcat tacttgtaag cttcataaca tcacaagctt ctttagcttt agatgaatct
     2161 ttaaatagaa tactagaaag cccttctgga gaaattacag aataaactga atgttctaac
     2221 atacaaacat catttcctac tccgatacct aaagctcctc cactaccacc ttcaccaatt
     2281 acaaaagtga ttactggaac agaaagttta ctcatttcaa gtaagtttct tgcaatagct
     2341 tctccttgtc ctctttcttc tgcttctaga ccacaaaaag ctcctgatgt atcaataaac
     2401 gttattatag gtctattaaa tttttcagct tgtttcataa gtcttaatgc ttttctgtat
     2461 ccttctggat gaggcattcc aaaatttctt tttatatttt cttttgtatc tcttcctttt
     2521 tggtgtccaa ctatagtaac attaaacttt cctatctgac caattcctcc aaccatagaa
     2581 gggtcatctt taaaaagcct atctccatga agttctaaaa aattagaaca aatatgttct
     2641 atgtattcca atgtcgtagg tctctttata tctctactta gtgttacctt ttcatatgct
     2701 gatagatgtg aaaaagcatc ttccttaagc ttttccaatt tctcattaag tgaatttatt
     2761 ttatcactta aatctatatt atttgcctta gaaatagtta ttaattgttt aatatcattt
     2821 tctaagactt ttattttttc actattattt tcaatcaaaa cagttcctcc tttatgccct
     2881 atttcttgtg catagcaaga atttgatata atacttcctt catttttctt ctatctacta
     2941 tcatgtcaat aaaacctttt tcaagtaaaa actcagaagt ttgaaatcct tcaggaagtt
     3001 tttgatttat tgtttgctct attactcttg gacctgcaaa acctatcaat gtatttggct
     3061 cagaaattat aatatcacca agcatagcaa aactagcagt tactcctcca gtagtagggt
     3121 cagttagaac tgatatatat ggcaatgatt tttcttcaag cttagaaagt gcttgagaag
     3181 tctttgccat ctgcataaga gataccatac cttcttgcat tctagctcct cctgaagcag
     3241 aacaaataat tataggtaaa ttattttcag cagcatactc tattgaataa gttatttttt
     3301 ccccaacaat agaacccata cttcccatca taaaatcagg attcattaca caaataactg
     3361 ttttaattcc atttattctt ccataacctg tgataacagc ttctgactct tttgtttttt
     3421 ccttataaga atctactttt tctttgtatt taggaaaatt tagtggattt tgaaattcta
     3481 catcagaatt aaactccata aaagttcctt tatcaatcaa taaatttatt ctttttcttg
     3541 ctcttaagcc ataatgtctt gaacattttg gacatacaaa tgaattttct tctatgtcct
     3601 ttctaaatac atgactatca caacctttgc aataggtcca aaatttatca tctacactat
     3661 tctttttaaa gttgtcatct aaagaaatcg taacatactt agattctttt gatgataaaa
     3721 atttttttat cataatctat ttactcctta taatgtagca aattctttct tcataaagac
     3781 atcttctata aatttggtat gatgagtatc ttctctaaac tcctcacttg ttacaagtgc
     3841 cttttgaaat tctacatttg tattaatacc ttctacaata atttcgtcta gagctctata
     3901 tattctactt atacattctt ctctagtttc agcccaacat attaacttac ctatcataga
     3961 atcatataaa ggtggtatag tatatcctgc atatacaaaa gtatcaaatc taacaccaaa
     4021 acctccaggt aaatttaatg attctatctt acctggtgat ggagcaaaat tctttgatga
     4081 atcttctgca tttattctac attcaatagc atgacctcta aaagtaatat cttcttgttt
     4141 aaaaggaatt ttatccccat tagctataga tatttgaagt tttataaggt caagatttgt
     4201 taccatctct gtaactggat gttcaacttg tattcttgta ttcatctcta taaaataaaa
     4261 gtttttatct ttgtcaacta taaactcaat agtacctgca ccgatataac ctactccttt
     4321 tactatatct actgcaattt tatgaagttt ttgtctttct tcatctgaaa ggtatggact
     4381 taaagattct tctattactt tttgatttcg cctttgcata gaacaatctc tatcacctaa
     4441 atgtattgca tttgaatgtt tatctccaaa tacttgtact tctatatgtc taggattttc
     4501 tataaacttt tccatatata aatcactatt agaaaatgct gctagagctt ctgaacttgc
     4561 catatcaaaa tttgaaaaaa gttcttcttc ttttctaacg attctcatac cttttccacc
     4621 gcctccactt gaggctttta tcataacagg atatccaatt tctcttgcaa cttctaaagc
     4681 ttcttcctta gttttcaaaa cagattctga accaggaaca acagggatat tcaattccat
     4741 catagtttct cttgctctag atttatctcc catcaaaatc atttgttctc ttgttggacc
     4801 tataaaaata atattattat cttcacattg ctttgcaaat tcaggattct ctgataaaaa
     4861 tccaaagcct ggatgaatag catcacactt taatttaaga gcaagataga ttatattttc
     4921 tatattatta taactcttac ttatattatt tggtcctata caaatagatt catcagctat
     4981 atatctatga aaacaatctt tatctatttc agaatagata gcaacagttt ttatacctag
     5041 ttctttacat gttctgatga ttctaactgc tatatcgcct ctatttgcaa ctagtatttt
     5101 ttttatcaac ttaaatacct cctactacaa tggttttatc ttaaataaag gttgattata
     5161 ttctactagt tcttcattac taactaatac ttcaattatt tcacctttaa tttcacttgt
     5221 tatctcattc ataagcttca ttgcttctaa tatacataaa gtgtcgcctt cttcaactac
     5281 atcgccaact ttaacaaagc tatctgcatc tggacttgga gaatcataga atgttcccat
     5341 caaaggagct actactactg atagattctc attagatgtg acaggtgtat cgtttacaac
     5401 tggcttttct aaagaaagat ccactactga ttcttgttgt acacttaaaa caggtgaact
     5461 tgtagattga gcttttttag atacttctaa tcttaaatcg ctactttcaa gttttacata
     5521 ttctaagtta gttgagtcta tggcctttaa taattcctta atttcattga tgttcatttt
     5581 gttaccccct tgtattatta cctggtgcta atattatatc acaaaaaaca aatttataaa
     5641 agaaaaagtg ctgactagag aatcaacaca tcaatatatc ttttaatttt aaattaacaa
     5701 ccagtaactt atatttgttt taattttcca ttattaatat aaattataag tataaaatga
     5761 ataaaattca atattttttg caaaatacaa gagaatacaa attaatacct cgggaatttt
     5821 taagacaaat accaatatta tgcaatttag acttcgcttt ctttcaaatt agtatcattt
     5881 tctttatttt tacataggat gtactttaat ttagctatca aaacattgtt atataggtta
     5941 aaagttatta gaattgcagg aattataaag taaactctag cacctataaa accaaaaact
     6001 actgtaataa gtattaatct tttatttaaa gtaaaaaaag gtatattgaa aatacttttg
     6061 tcagatgttt ctggagtata agaatatata actccaaaaa tcaaagtaag catacaaagt
     6121 ggaagtagtg tataacttga aattattccc agaaatccta tactttcaaa atatttgcta
     6181 gcactatttg tataaacaaa catgtatcca tttaaatatg cgcatataca tgatataatg
     6241 ccaaaagttc cccctaagga ttgagtgtac caattaaaaa atttagattc catatacctc
     6301 acctcctaaa actgtatata taatgatatg catttaaata gtaaaatata tctttatatg
     6361 tttctgaata aaattttgtt aatttcatta atttatgttt aagaaatttg aagtatagtg
     6421 tagtatatat taatgtagat gaagttttat agtacttatt tcatagtgct taatcaagtt
     6481 atcttattat ggattcaaaa gttgtttgta gggttttata ttttgttgag ctgaaaaatt
     6541 tattataaat gaagggatgt tttcgctttg gtagatacat tagaatttgg tcttaaaatt
     6601 ttatttttta tattatcaat aatttggatg ggtaaaataa tgattttaag aactgataag
     6661 caaatagtaa tcaatccttt gcttattggt atatctgcag tccttgtgat gttgcatacc
     6721 tcacagagta acattgaatt ttttggatta gatgtccaat atataagaat agttttgtat
     6781 ataatatatt cactgataat tttaatagga atatgggcta ctaacagaag aaatggaatt
     6841 ttttagataa tctatttcta taaaatttaa aggttgctag taacaaagca ctaatattag
     6901 cgttctgtta ctaacaacct ttattttgat tatacactta aattgaaaat agaatgattg
     6961 aataagttga cagattattt tacattgaca gtaattagaa cttcttgaga tgtatttaaa
     7021 tcatttttgt cgcttagtat aacttttacc actgcttgac ctgttttatt acctttaatt
     7081 atcatatctt cacatgaaag tacactctta tcacttacag atacaataaa atttaaatct
     7141 tttggaaggt tatcagtaac ttcccagtat ttataataac tatcaatttt cttcgtaatt
     7201 atttttctca aatcaatact ttcacctttt gctatatcaa cattatgata tttagagtcc
     7261 agtttatact gattatcttt tgtatcaata aaatttaaat cgcaatttac atccacacca
     7321 ttatgagaaa gagaatttac tacatcatca tcaatcaaat aattattggc aagatttatt
     7381 gaatccatat cttttagact taaagcttcc ttaggaacct cttttatatt attattccaa
     7441 aaaataaaat tgtctacatt ttcaagatat gatatacctt ttaaatttga aatgctctca
     7501 ccagttgcat aaaattctcc atctaaagcc tttatattgc ttatagtcat ctcatctgga
     7561 tttatatatc tttttacaac tgctcttaaa tttgcatcag gaaaaatatc tttaagttga
     7621 ttttcgctca aaaccttatc atttgaattt attggactta aagctgctgc agaaataata
     7681 aatggtatta aattagctaa attcatagta ccctcccaca taaatttttt attataataa
     7741 tttaatttct atgagagggt tctgtttcct tgttatatat acctactttc gtacacttta
     7801 ttaatataaa ttaaaataaa tttctactat taaacttata aacatattac tagttacgtt
     7861 ttttagcttc acatctaaat attcgcatac cttgtgacat aaatttgtct tcatactctg
     7921 ttgtaacatt attttcatac tcactatttc ctaaatcaag agaaatattc tttaatagcc
     7981 aattatttgc agcaatttca tttaaactaa actcgaataa tttttcatta tctgttttaa
     8041 agtgaatttc gccatcatct tccaaaaccc tattgtacat ttctaaaaat ccagaatgag
     8101 tgagtcttct tttactccat ctatttttag gccatgggtc acagaaattt atataaattc
     8161 tcgataattc tttagcttca aagtaatcca aaatattgct tacatctccc cataaaaata
     8221 gtatattatt caaattagaa gcatctgctt tttcaacagc ctttaaaaga acttcttcct
     8281 taagttccat ggcaatataa tttatgtcag ggttctgctt agctaaagtt gttataaact
     8341 taccttttcc agtcccaaat tcaacatgta ttggattatc atttctaaac tttaaattcc
     8401 acttgccttt taatttgtct atatcgtctc ttaaaacata ttttgtataa cttaaaagct
     8461 tttcatctgc accttttttt cgtcttcttc tcacaataat tctccttaca tataaaaaat
     8521 tagtaaaata catttttatt atacattatc tcaccaaaaa ataaaaagaa ctctcttaaa
     8581 atagaaattt atttctataa agagagttct aaacttacta aaaacttaat cttagtggca
     8641 ccctgaacaa ccagagcatc ctcctccacc ttgatctttc atattttcag gaataactct
     8701 aaatccaaat cctgtatctt ctattattat atctccatac tgactatatt catcagagct
     8761 cattacaaat tgtaattctc caacttcata agttaagtca tcttcttttt tctcatctaa
     8821 agctaatcca aaagaaggac ctccacaacc aacacctgca aagtatactc ttatattatt
     8881 aggtttatct tgattgtctt ttaagatact ttttaaagta tcaatagctg tttctggtaa
     8941 agtaattttc atcaaatcat tccctttcaa atattttatt aaaacaatat ctattatata
     9001 cccatacaag gtacataatc aattagtttt tgcatatttt actaaaactt ctacctaata
     9061 ttacctaagg ctttaaaata ttttatataa tattttatta ctttaaataa ataccctatc
     9121 ataaacttag taaacaaata aaattattat tctaaaaaat ataataattt tatttgttgt
     9181 tttaatatta taacacattt gataatgtcc aattcatata tgcaaaattt atatacttta
     9241 aaatatctaa gaaattattc aataaaaaaa tactattttt taaaaattat cattattgat
     9301 aaagctctac tatttatatt ttttagtgta ttcatgtata ataagtaaaa gcacaacggt
     9361 atatactgtt gtgctttttt gtaattaata aacaatattt tggtctataa caatgaaaaa
     9421 tggctacttt ccataattga tttcatctga cataagcaaa aagggtgtcc agctgggtca
     9481 atcataactc tccagtcatc agtaaattgc tcctctgcga ttgtagctcc acaatgaatt
     9541 gcatattgaa ctgcttcttc taaatcatta acagcgaaat ccatatgagc catttgctgt
     9601 tgagattcag gtttttctgg ttctattatg ccccaagcct ttgttttttg caatataaag
     9661 aaaaaattat atattttaat attttataat tgttgaaatt gtgtaataaa ttcaactgga
     9721 tacatagtgc gttttgaaat ttcttctaaa gctaaacctt gttttaaaag cctctttatg
     9781 acgctttcca tactttctga aatttctata atatttttat caaaatcata aaacctaaaa
     9841 gttctttgac cccatgggta ttcttttata taatgcaaaa attcaatatt tcctattcta
     9901 tttatcttac tttcccattg tttgatgtca ttaacctcaa aatacaactg aaagttattt
     9961 gattgttgat atattggtaa atcatatcca attatagctt tataattact ttgtaatcca
    10021 aatccatttt caaatgatac atgttctcct aaagctaatg tgattttttg ctccataaca
    10081 tcttcataaa acttttttga tttatttatg tctttgacaa ttagcaatac tcctccaaat
    10141 ttcatcttat cactatcctt tctttgttat aagtattata acttaaaatt ttgcaatttc
    10201 actgtaaaaa tcagacatat tagctcggta tttttgagga gttattccac agacaatttt
    10261 aaaatcttta ataaaatgtg gtatatcata gaatcctaat tcattacata tagacatcaa
    10321 tgaagtttta ttattgttta gaagttttat agatttatta atacgtacca aacgagaaaa
    10381 tgacttcata ctcaacccta ggtactgatt aaagagtctg tttaaatgcc tagaactata
    10441 aaatactttt ttagaaattt cttgtgaaga tatatttcca gagttttgaa taatcaactg
    10501 aattgccaaa ataaactcat atgagtattc aattttaata ttagaaatca ataattcttc
    10561 caactttaaa atcaatttat ctatagataa agattcatta aatatttttt taatttctaa
    10621 atctaatgaa gtattaatta tagaaaatgg aacaacttta tcaataagtt cactttgctg
    10681 tatacctata agcgggaaaa aaccagcagg ttgaaattct ataattagta taacatcaca
    10741 tctattagca atatttccta ctgtttttgg tttagtagtt ggaccaaata atagactatg
    10801 taaccctgtg gcatcataaa aaaacaccaa agttacactt ccatgaggaa taatcgtgta
    10861 attatcagaa attattgttt gatttggaaa cgatatagta aaattcgata tccaatttct
    10921 taattgcata caaggcaaga gataaacaaa attgtcttca caaactataa gtgacctatt
    10981 tttatttata aaatgaatac tttctttttt taacatatat tatatcctgt cctatcgtga
    11041 atatttatta aagtaagaag aatccatatt atctctatat atcattaact gcttttaata
    11101 atagagatag tatgaatact cattagatat ttaataaaca aataaaatta aaataatcaa
    11161 taaaaaatat agtactttta tctgttattt taatattata gcacatttat agtatttaac
    11221 ttatatccac tttacatatg tctaatttat ataattaaaa atgcttatct caaatacaat
    11281 aattagtcct tatacaatta aaccatctga aactgtaata gtacgactac tacttttggc
    11341 tgcttctggt gagtgagtta ccataattat agtttgtcca ttgtctctgt ttattttatt
    11401 cagtaaatct attatttcta tccctgtttt actatctaaa tttccagtag gctcatcagc
    11461 aaataaaatc tctggttttc caattaaagc tcgagctata gcaacacgtt gttgctgacc
    11521 accagataac tctcgtggtg tatgtttacg tctatcagtt aaaccaacta tatctaagat
    11581 ttcatctaat tgatttttat aatcttttaa atttttacca tctaataaca aaggaagcat
    11641 tatattttcc ttcacattta aatttggtat taagttataa aattgaaata cgaatcctat
    11701 atttctacga cgtattatgc tcattttctc atcattaaaa tgtgatatat cagctccatt
    11761 catatacact tttccgctcg ttggagtatc taaaccacct aaaatatata aaagagtact
    11821 tttaccagaa ccagattgac ccatgataga aacaaattca cctttcatta cttgtaaaga
    11881 tacctctttt aacactcgag ttgttgtatt tccaatttta tattctttga taatattttt
    11941 tgcttctaca gctaaactat tatgtgtaat tttcatatta acctccataa ttattcaaac
    12001 ttaatttctt ctatcagttt cattttcttc ccttttataa taggcactat cgaacctatt
    12061 aaagttacta taattcccaa taacccaaca ataataaatg ttttaaaatc taaatctggt
    12121 ttcattgaaa tttttggtcc tgcaactata aagatagttt gaatctcaag tatagatatc
    12181 acaataccaa taacagctcc aagtaaacct gaagtaaccc cttcaatcaa tgttactttt
    12241 atattttgtt tattacttag tcctatagat ttatacatag ctatagaacg tcttttttga
    12301 atataattaa tcagtagatt attaataata cctactgtag caagaagaaa aatgaaatag
    12361 gtcatcttat tcataggaga caaaaaacta cttatagtat ttaaagaatc attattaaat
    12421 tcttcaactg tacgactcca attatatgta tctccaaata aatatcttat ctgaatcata
    12481 acagcatctg gattgactgc tgtatatact aaaaaaccat aattagtttt atcaaaatca
    12541 ttaactgcat aatgtgatgg tataacagct tctacatcat ttgcacggga tttaaaacta
    12601 cctactattt tataagaaaa atcatactta tcattagata gagttattgt atctcctata
    12661 gataagccta ctttttttag agtatcttca ttcaaaataa cagaacgttt atcattaaaa
    12721 gcttctatta cttgtttttt tatctcaaaa tttgtataat tgattccaaa catagagtta
    12781 tatagtttta tattatctgt accctctagt cttgataatg ttacaccatt accagaaatc
    12841 tcattattca taacataaag aggtaatatt tttttaattc cttccatatg tctaacatct
    12901 tcaataaact cttcattcat ctttccatct gcaaaacctt gtaattctgc gtcacgaaac
    12961 acatctgtta tataagtttt gacaaagtta ccaacaacac ttattgcaat aactgcagaa
    13021 atacttataa atagtaaagt gatattctgt attatatttt tgttattttt catatttcta
    13081 gcagctaatt taccctcatt ccctaaaata tttttataaa caaactcaaa aactatagac
    13141 atgatatcag taataagagg aatcaacaca atagtagcta caattaatcc tagtaatgaa
    13201 aatccaccag ctagatacaa tgtattttca ggggatattc ttggaagtaa aatagataaa
    13261 ataaacatta tacttcctat aaaaagtata gaacgatttg aaacattctt ttcttctact
    13321 gttcctagta caatattttt tatcggcaaa tggctagctt ttttaacagg tatatatgca
    13381 ctaaatgaag aaacgattat tgctactata acagatataa tcactccaat gggtgatatt
    13441 actactggaa ttgatattcc ttgttcgaga gaatttccta acccatgaag cattaaattt
    13501 aaaacaacta caccaatagg aatagcaatt aatcctccta tacttccgta taatatactt
    13561 tcaagcatca atatacgagt tacagttttt tcattggcac caatacttct aaatgttcca
    13621 ataaaaggaa gacgttctaa tgtaataacc ttataactgc tgtatattat aaatatacta
    13681 agggttaatg caaaaaaact tattaaaaag aaaggcattg tcttttgtct agcatctgaa
    13741 attatcttag tttcatttat agtatttgat accatatatt tttcagtaga aagttcttcg
    13801 gataatttat ttactagatt ttctgtaatt tcttcttctg actctataag aactcttgta
    13861 tatccactgc ctttatttat aatttttgat agagtctctt tgggtagcag tgcatttaca
    13921 cctcttgtgt tcctcaaaaa tactgtatca taagatgcaa tatcactaac ttgaaaagta
    13981 taagactttc cataaatctg caaagtaata gaatcacctt tttttatttt atattttgat
    14041 gtaaagcgat ttggaagaat aattttatca ccagaaaaat cagtaatact atcgccattt
    14101 tctaatcgtg gcttattgat tttatttagt tgacttaagt ctgctgatat aagactaaat
    14161 tcttcatagt atcccccttt attataaata gcagagcttt ccaatacacc aacctttgat
    14221 tttatagcat ttaaatcagg tatgtcttct aaattggtta atatatctgg atttttactt
    14281 tggacagata tagtcgctgt accatacata cctttttcca tttttctttg tgcactttca
    14341 taagataatc caattgagaa tgaaacaaaa agtaaaactg ttgataaaac tatggacaat
    14401 aacataactg ctgttctaat ttttcgttct ttaatatttg tgaaaatata ctttaatata
    14461 attttcaaat ttacaccttc tttctttatc taagatattt caatctaata tttttatact
    14521 ttaatctaaa ttaaatctct aatagactcc tccatacttt tatcattcta tttataatat
    14581 ttttaatcaa atcacccctt aaataaaaaa gattgataaa ctactatata aatataataa
    14641 tctatcaacc ttaatatatt ttttatttaa ccttaattaa ccttaattaa ataggaaagg
    14701 tgatataaaa tgagctacct aatccaggta tactttcaac agatatattc cctccatgaa
    14761 cttcaataat ttgcttcgtt attgctagtc ctaaaccagt tccttctatt ttttgttttg
    14821 tattgcttcc tctataataa cgattaaaca aatttgatgc ttcttctctt gacatccctt
    14881 ttccattatc agatataatg atttcaattc tttcatttaa tgttatatgt acacaaactt
    14941 tagtattttt atctccatat gtaaaagcat tgacaataag attactaaaa gctcgtttta
    15001 atagtgtaca atcaaaatca aagttaatat tatctatatt actcttaaat tcaactatat
    15061 ttgattcata ttctggataa tttaaaatat caatggataa ttctttcaag aatcttacta
    15121 aatcataatt atttttattt aaagggatta ttccattttt taattgatag gttaatttta
    15181 aatcgtttat aagtgtattt gtatattcta tattctttaa catgattgaa gcatatttat
    15241 atactttttc aggtgaataa ttagtactac tatctgtaag aagctctgca taccctttta
    15301 ttggtgaaag tggagttttt aaatcatgag ttatatttga aatccactct tctctcataa
    15361 tatcagtttt attttgtatc tcatcacttt tatgaatttc ttcatttaaa atatttaaac
    15421 tattatatat atcagcaaaa acacctttaa tattaactgg aagatagctt cgttttgaaa
    15481 tatcgctaac agcagatgta atatttgata tgcactttgt tacccagtaa ccataagcaa
    15541 ttccagaaac caaaattatt aaaaatataa tccctatgat tgaaaataca attgacttac
    15601 cacctataaa tttacctcca tcaaaaaaca tattcacttt agatatattt aatggaaaat
    15661 atataatata aatatagtca tttttattat ttttaattgt attaaagtat acagttccat
    15721 tacttgaatt attttggtta gctaatgcta ataattcaga attagaataa taatctttat
    15781 gctcttttgg ttctaaaaac ccataaactc tatttccact agaatctaaa atttgcaacc
    15841 acaattgatt ttctcgaagt ttttctaatc cagactgttt tacttgtggc ttattatcta
    15901 taaatacgat ttgttcatta aagttttctg taaatttttt cggccagtca ctacgaataa
    15961 attccccatt tggtttctga atagtaataa gtgataagaa aatacctatt actgtcagga
    16021 ttaagcaaat aaaaatcatg aaaaaaataa tgtatgaatg aaatactgtt ttataactag
    16081 gtttatgcat ttaatcaact cttttcttaa acttttattt tgattgttgc ttatataaaa
    16141 tagacaataa ttattgatat gtaaaaattt atttaaaaat acaactatct acataaatta
    16201 tatttatata attatatgtt atcaagctgt ctgttgtatt taataaatcc ttttgataac
    16261 atataaaagt aagataaatt tttccattaa ataccttata attatacaca aaatctctat
    16321 atacgttaaa cagtatagag aaaattttca tagaaccatc tttatagggg atactttata
    16381 aaagaataaa aaaattataa tatataaaaa cttcatagga gtgtaacaac atcagtaaag
    16441 atatgaaaac actatataaa aattcaaaaa tattaagaat aatgaaatac ttgaaattgc
    16501 ttatatcaag aaattttata taatataaac tggtcaaata tgttataata ttaaattgtg
    16561 acaagttcat ggaggtgttt atatgaaaaa acaaaagtcg ccaattttac ttgaaatgct
    16621 tggactattt tttggttgta tatcaatgag tattggtata aatatgtttt taaaaccaca
    16681 tacaattgct cctggaggac ttagtggtct atcattagta ctaaacaagg ttacaggact
    16741 tccagtatca gctatcatgt taattatagg tgtcccatta gtaatacttg cttttagaat
    16801 tatgggtaca aaaaattcat taaaaacttt atttggaaca gttgtatttt cagctatagt
    16861 tcaattaaca gacccattat caaaactaag atttactaat gacttattat tgtcttctat
    16921 tgcaggagga atcctagttg gtataggttt aggaataatg tttaaatcag atgcttctac
    16981 tggaggaaca gacttaattg cactcatact tagtaagaaa ttcccaggaa taaaagctac
    17041 aaaattcatg tcatgcctag atggaatggt tgttatatct tctggtatag taaatcgtag
    17101 tttagaaact gctctttact ctggtatagc actttgtgta ttaataaaaa tagcagatat
    17161 gataatggaa ggatttgacc attcaaaagc attttttatt atctctgatg agcctgaaag
    17221 tttaagacaa gctataactg aagaacttga tagaggtctt actattcttg atggtaaagg
    17281 tggatacact agagaaaata aagaagttct attagtcgta gtttctaaaa aacaagaact
    17341 ttatctaaag agattagtta aaagaactga ccctactgca tttgttattg ttacagatgt
    17401 acatgaggtt cttggagaag gatttaaaaa tttagagaac taataaaaat taatatttta
    17461 agtatatata ctattttaat taaaatacga tttattcaaa aagagtgtct cataaacttt
    17521 ttagttcatg agacattctt ttttgtataa aatcaaatgc attttcagta ttttaacaat
    17581 aaaaacagga ttatctatat tttgaggcaa ccactttatt gaaattttat ttagaaaaca
    17641 atcattacta aataaccagc atagatatta aatgattata tctattgttt tttcaattca
    17701 tattgataac aaggaacatt ttttacagta tttccagtat gaattttttt gatatacttt
    17761 cctgtacatt taaatcctaa taaatctaat gattttgtag aattttggtt ttcttcatag
    17821 gttattgcaa ttattttatt taagtttagt tctctaaatc catactgtat tactcttttt
    17881 gttatctcag tagcaaaatc atgtccccaa tagcttggat ttatacatat acctatttct
    17941 gcttctaatg ctttaggatt tatattatta aaccccatat ttccaataac tttattgtct
    18001 cttttatata ctatagcaaa gtggccaatt ttcccttttt tatatggatt tagaaaaaat
    18061 gattttataa actttcttgt atcttctaaa gatttatgct cactaaatgg taaatgtttt
    18121 actactattt tatttttata acagtcaaaa aaatcttcaa catcatctaa tgatgcaggt
    18181 cttagtatac aacttctagt ttctaaggtt ttcatttgag gatattcaga atcagacatt
    18241 tctaatcccc ctttatacat taatctaaat atactgttca tatttaaaat acgatattaa
    18301 ttatataaat gttctcaaat taaccttatt tttattttta ataaaaacta attactatat
    18361 gaatctttca aatttacgaa tttatctcta tttttatatt aaattacttg tgaaatatgt
    18421 attctaaatt atatattatt agatatcatg cttaaaaatt ctttggttgc taaggataaa
    18481 gatatatctt ttaaatggca gtaacctata tttctcttag gtattggctc ttttatatct
    18541 aatttaaaca atttctcatt ttccaaataa tctatgctaa attcttctat aacacaagac
    18601 actccaagat ttatatacgc aaattctaaa agtaattcat gagaacccaa ttctatatct
    18661 gggtttagtt ttataccttt tgataaaaaa tattcattta cataaagtct tgaatttgct
    18721 ttattttcta gcattattaa aggcaatgtg tttaattctt ccaatgatat tttcctacct
    18781 ttgtattcta tgtaatcatt tgcacataca aatatatcat gtatttcaat acactcaaca
    18841 atatttagtg tatcatcttc tattggcatg tttataattg ctatatctat attcccatct
    18901 ttgagtaaag atataagttc tctactcgtt ctatttattg tagatacatg tatatgtgga
    18961 tacttcttat gaaaaatttc taaatatttt aacaagaaaa aacgtgtagc tgtatctcct
    19021 actcctattt tcagagaacc gtattctaat tttttaaatt tatttattct atcttctcct
    19081 gatgatatta actttaatgc agtagtgaca tgttcactta gaattttccc ctctggagtt
    19141 aattttactc ccttattatt tctataaaat aataaagtat ctaattgttc ttctaattgc
    19201 tttattgact ggcttacagc tggctgtgac ataaacaatt ccttagcagc aagagaaaag
    19261 ctcttattat ttacaactgc attaaaaacc ttataaagtt caagttttat attcatatat
    19321 aattactcct tatgtcaaat attagtatta tttattttac ttataattat atttgaatta
    19381 taatatacat ggtactaata tgacaatatt aattctttag gaggatttta gataatggat
    19441 agattagttg gaactgtttc aagaggggtt agagcaccta ttataagaca aggagatgac
    19501 ttagttaaaa tagttgttga ttctgtttta aatgcgtcaa aaagtgaaaa ctttgaagtt
    19561 agggacaaag atgtaattgc cgttacagaa gctgttgtag ctagagcaca gggaaattat
    19621 gctcacgttg acaatattgc aaaagatgta aaagataaat ttggagatga tactgttgga
    19681 gttatattcc caattttaag tagaaatcgt tttgctatat gtttaaaagg aattgcaaaa
    19741 ggatgtagaa aagtcgtatt aatgttaagc tacccttctg atgaagttgg aaatcactta
    19801 ataagtattg atgaacttga tgataaggga ataaatcctt ggtcagatgt tttaacagaa
    19861 gaaaaataca gagaactatt tggatataac aagcatacat ttactggtgt agattatgtt
    19921 gattattata agaatttaat caaggattgt ggtgcagagg tagaaatagt atttgcaaac
    19981 aatccaaaaa ctattcttaa ctatacaaca agtattttaa cttgtgatat acatacaaga
    20041 caaagaacaa agagaatttt aagacaaaat ggagctaaaa aagtatattc acttgatgat
    20101 ataatgacag caagtgtaga tggtagtggt tacaatgacc agtatggact tttaggttca
    20161 aacaaagcta cagaagaaac agtaaaatta ttccctataa attgtgatga agttgtaaac
    20221 aaaatccaag gtaatataaa agaaataaca gggaaagatg tagaagtaat ggtttatgga
    20281 gatggagctt ttaaagaccc tgtaggtaaa atatgggaat tagctgaccc agtagtatct
    20341 cctgcttata caaaagggct agagggaact ccaaacgaag taaaattaaa atacttagca
    20401 gataatgatt ttgctgactt atctggtgat gagttaaaag aagcaatttc aaaatacata
    20461 gttgaaaaag ataataaatc tgatgattta acaggaaaca tggtttctca aggtactact
    20521 ccaagaagat taacagacct tataggttct ttagcagatt taacatctgg aagtggagat
    20581 aagggtactc ctattattta catacaagga tatttcgata actatacaaa atagtatgtt
    20641 ttaataagat ttaaattttt tggattatat atattaacat gaaaatttat taaaacctct
    20701 taatagatat gacttttcaa gagttatatc tattaagagg tttatttaat gttcacagat
    20761 aagatatatt taaataattt aaacaagatt gtcagaaagt tatataatct taaaaattta
    20821 tataactcta attcttattt atttctgaaa taatatcact cttcaaaatt tgtcttactg
    20881 gaataattac agcaactata ctaatagtga tatttaaaat aatcatacct atgtataaag
    20941 gtgtatttat tttaagaatt ggatttatat ataaatatac acgttttacc ataaaataaa
    21001 tcataaattg tccaataaca cttccaatta ccattataat acttgcatag aatccatata
    21061 aaaaaccttc tcttatcatc ataagtaaaa acttattatc cgtaataccc atagctctta
    21121 aaattccaaa ctcatgtctt cttgaaaaaa ttaagtagct tactgtattg attatatgga
    21181 acaaacttat tgcaagaagt acaaatacaa taccatatat aaataagagt ttttgattta
    21241 agtaggtatt atttttttca atagcaatgg tataatctgt tacgatacat ctttttacat
    21301 cacttacaac actttttatg ttctcagaca attcctcact gttactatct tctgtctttt
    21361 ttatacttac tgaattataa ttttcgatat caaagttatt tttcatttgc ccattcgtca
    21421 taactatatc cataccataa tcaccaatga aatatatgtt atttgccatc acacgtttta
    21481 cagtagcagc aactacaaat tcttttgttg tataatcatt cttgttttct tctttaaatt
    21541 taacaatttc atctgttggt ttcattgtct ttggcacttt aacttttatt gtgtctcctg
    21601 gatttatatc aactgcatca tacatcccag ttccatccat aggtagtcta actataacct
    21661 tattattttt aaccatatca tgggtattaa tctccccatc tataatataa gaatttaatc
    21721 cttctaacat accattgtta taaccataaa tatttgtttt taaaagatag ccaccttcat
    21781 tttgctttgt acaaatacca tcaaaaaaag tctttatcct gtaatctttt tcaagaggtt
    21841 taaagaaatt tttccaaaat agttgttctt ctttaatttg catagctcca aataaatatc
    21901 ttactggata gacttttgat attccctcaa cattgtttaa tcttgaaatt ttatcttttg
    21961 gtatacctac atcaaaatct gttgtttgca tagttatttg gtagtcagaa tttaaaccat
    22021 catcagcttt cattgtaagt tcattattat ttttagtaag ctgtatagag taactagaac
    22081 ataaaaatat aattccacca agtgataatg aaaacaaaat tccaataaac attccctttt
    22141 tttgagtcat gtatttatga gataatactt ttgtcatgtt agagtgtttt acactttcta
    22201 atattctctt tttcttatta gtaatcttaa agtttttcat agattctata ctagagtatt
    22261 tattcaactg atgtacaaca cgtacagcaa ttaaaattaa caacaataag ataaataaaa
    22321 agcctaaata aatagatttt tcacttataa aaaatgctcc tggaacaata tcactactca
    22381 aaaatatatg tggaaattgt tcatatattg tagacgctac tccaatacct aagaagcaac
    22441 ctataggaaa tccaacaata aaaagtaaaa ataactctaa aaatagtaga aataaaagtt
    22501 gtttattatt agcgcctaat acttctaaaa ccccatattg tgatattctc tgaagtattg
    22561 aaacatggaa aatactatat actgctgctg caccaaacac tccaacaagc aatataattg
    22621 catttgtatt tagatctaaa ttttttaaga actcaaatat attatatttt tctatatttt
    22681 ttggtggttt tactccaaat tcaactgaaa gaggccagtt tatataagtt ttttcttttg
    22741 atatgttgaa ttttttcata aatttataat tttgcttttt aatattttta ttttcatcat
    22801 attttacata aactatataa gaatcctttt ctttaggctg aaaataatcg tttacaaatc
    22861 cctccagagc agtttctggg tttgaaagac ttgataaaat accacataac ttatagattt
    22921 taccatctaa attgatttct gttccaattt ctttgtttac atttaaattg tttagagaat
    22981 atgtgtcaag tactatctct ccatctttac taggaagagt tccttttaat aattttctac
    23041 ctgtcatatc taaatatgaa gaatcagcat ttaaaaattc tataataaaa ggttcatcta
    23101 tctgattttt aggttttgta acgccaagtc tatctacagt gtattccttt gattttttat
    23161 tttctttaat tttattaagt tgttccttat ctactttata gaaataatga taatctccac
    23221 tatcttccct tatcttttca attctacttt tgtctgcact atgaactaaa gaaccaatcc
    23281 ccgtcaataa tgctactgat agtactatgc ttaaaactat agatatagat tgttttttat
    23341 aatgctttat atattgaatt gaaaatttta ctagagacat catagtactt caccaccagt
    23401 attcaccaca attccatctt caatatggat aattgagtcg catacttgtg ctatattttc
    23461 attgtgtgtt accataagta tcgtttgatg gaattttgca ctactttctt ttaataaaca
    23521 taccacttcc attgtagttt tactatcgag atttcctgtt ggttcatctg ctaatataag
    23581 cgcaggttta tttgccaaag ctctagcaat tgctaccctc tgttgttgac caccagatag
    23641 ttcgcttgga aattttaaac gcttatccca aattcccaat gtcttcatca gcatttcaat
    23701 atattcatgg tctatatggt ttcctttatc taatgtaaca ggaagagcta tattatcata
    23761 agtgtttaat acgggcatta aactgtagtt ttgaaatata aatcctatat ttcttcttct
    23821 aaatactgtc aaatcttttt ttcctatttt agatatgttc tttcctttaa ttattacatc
    23881 acctgaagtt ggattatcta atccacctat aatatttaac aatgtgcttt ttccagaacc
    23941 agaagttcca actatagcag taaatgtatc tcgttcaatt tctatactta ctccatttaa
    24001 ggcttttact ttagtctcac cttttccata gtgttttact aaattattcg tcactaatat
    24061 actcatttaa tttctccttt ccatacagat atatgttcta taaaatattg taagtgacga
    24121 taattacact catcctacaa aattagttta aactcttaca attctgtaag aagatactaa
    24181 aacaacatcc acttcctagt ttagattcta ctataatgtt tccattttct ttttctaaaa
    24241 ttaatcttgt aagatataga ccaatccctg tgccattttg gttttccaca tttttacttc
    24301 tgtaaaatct tttaaaaata tcatttagtt ctctactatc aatacctatt ccctcatctt
    24361 taatagtaat cttcgtatat agttccattt ttattacaga aatagtaatc gtgctatctg
    24421 atggtgagta ttttattgca ttttctaaaa catttacaat tgcttctatg gtccactttt
    24481 tattatgaac taattttatg tctgaaaatg gttcgagatt tatccttata tttttagctt
    24541 ctgctttatt aaagatagta ctgatacttt gaataagagt atctttaatt agtagttctt
    24601 ctacttcaaa ttctataact ccatcctcta atttagtcat tttaaataag gattgtaaca
    24661 gccagtcaat cttaaaagtt tcatttttca tcttttgtaa aaactccttc ttctgtaatg
    24721 tggaaatatt tatattctcc agaagttcac aataaatggt tatatttgaa agtggagttt
    24781 ttaattgatg tgacatattt gatattaaac ctttaatcgc ttctttttct gactttgact
    24841 gttctacttc tatttctatc atatccttaa ttttttttac ttggtgagaa atttttgacg
    24901 ctcttgtatc cttcgtatct ggataagtaa cttctttacc ttccaatatt tgatttaaca
    24961 tcaaatttaa ttcatcatat cttctttggt ttattttagt agatactata aatataacaa
    25021 ctgtacatat tataaaggct aatataaaca tattattcta cctcattcca taaataaccc
    25081 attccataag ctgtttttat atattttgga ttagatgggt catcctctac ttttactcta
    25141 agtcttctta tatttacaga tacaatatta tcatcaacat aattttcttc gctgtcccaa
    25201 atatggtgta aaatttgttc ctttaataat atttgacctt tattttcaat tagataactt
    25261 agaagcttat attctacaga gctaagttcc aaacattctt tatttttata tacttttagt
    25321 aacttttggt ccaattttat tccattggaa tatattactt tattattcga tttttttctt
    25381 attgctactt tgactcttgc ctttaataca gataaagaaa atggttttgt aatataatca
    25441 tcaagcccta tttctaatcc ttttacttca tcaatttctg agtctcttgc agtaagcatt
    25501 ataattggta tatctgactt ttcccttatt atttgacata catcaaatcc atctccatct
    25561 ggaagattgc agtctaagat tataaaatct atatttttac tattaaatat atttaatcct
    25621 tcttgttttg tataagcctt aaatacatta aatccatcat tctcaaatgc ataaaaaaga
    25681 ccttcattta agtttatatc atcttcaata attaataagt tcaattttac tctcctttag
    25741 attttttaga taaataatta ttttcttgca ttatcatact aaaaatattt taattttatt
    25801 tatttcacta aaaagattgt atttaaattt gttttcaaaa taaaaacatc ataatcaagc
    25861 atatagttat atatttccca atttaattac gcttaaattg aaaatataac tttattctta
    25921 tataattaaa ttatacaaca ataaagttat attttttaaa tatattttta ctagggggaa
    25981 tagaattgaa aagggttgat gagagaaata ctatgtttgc aagagcaaat tacaaaaaag
    26041 atagtactgc ttataatgac tattataaga aaaatcctga caaaaaagaa attgatgata
    26101 gtataagaaa tagaccaaat ttatgtagtg aaggaacaat gacttacaat gagttaaact
    26161 ctccaatggc aagtagtgca ttcgattttt tatctgatat caaatcttta tgcgaaggaa
    26221 aagtttctga tactaaagta gatgtagatg caaagtcaat gacaaaaaaa attaagggtc
    26281 ttgcaaagca gtatggagct tctgtagttg gtataactaa attaaaagac tatcatattt
    26341 atacaaatag gggtagacat gaagaaaatt acggtgaaga aattaatcta actcataagt
    26401 atgcaattgt atttggatgt gagatggata aggaaatgat aaatagagct ccaatgatat
    26461 gtgaagttat agaaacatca aagtgctatg tggatgcatc tatcgtcggc atgattttaa
    26521 gttactatat aagaaattta ggatatgatg ctagaaatca tatggatgct aattatttag
    26581 taatgcctgt ttttatagct cgtgatgctg gtttaggaga tataggaaga aatgctatac
    26641 ttacaaataa agattatggc tctagactta gattaggtgt tgttactaca gatattcctc
    26701 ttttagaaga tgaatatgtt gattttggac tagaagattt ttgtaaggtc tgtaaaaaat
    26761 gctcttttaa ctgcccttct cattcgcttt ctaatgatat taaaattggt gatgatggaa
    26821 aatataattg ggtaattgaa catgagacat gctatattaa atggagatat ttaggtacag
    26881 attgtggtat gtgtatatct gcttgcccat ttagccaaaa tctcgaaact ataaagaata
    26941 ctacttcatt taagggtaat aatgagctta ttcaaaaagc tttagatgag tatacttcaa
    27001 aatttggtaa aagaatattt atacctggaa atccatcttg gttgaaataa ttttatttaa
    27061 taaagaatct gtactagaat ttatcatagg acaggttctt tattaaatat ataagttttt
    27121 aaatactttt tctcgtttat aacaattcag caatgttttt agtctataat tgacattata
    27181 aaatattaaa ctaaaaggag taactgtatg gaaaaaatat tagtagtaga agatgattct
    27241 atattaaata aaactctctc atataaccta attgaagatg gatatataat tacttcaaaa
    27301 tttacagcta aatctgcact gaagtctatt tttgaatgtg aatttgattt aataattttg
    27361 gatattaatt taccagataa aagtgggttt gaattatgta atgaaataaa gggaaattac
    27421 aatataccaa taattttttt aactgctaat gatatggaat gtgatatgat taaaggctat
    27481 gaacttggag ctttggatta tataacaaaa ccatttaata tcaatatatt taaacaaaaa
    27541 attaaagctt ttttaaatca tcttataata aaaacaaaac aagattacta tagggatgga
    27601 tatcttgaaa ttaacttttc tgagttatct gccaatataa atggaaatca aattatattt
    27661 acaccattag aatatcgtac tttaaaactg cttactgaaa atcctaaaag tattttaaca
    27721 aggaaagtac ttttagaaaa actctgggat atagatgcta actttgtaga tgaacatact
    27781 ttaacatctg ttataagtag gattagaagc aaaattgaaa aagacaatct taagtatatc
    27841 aaaacggtat atggtatggg atatatgtgg ttaggtgaaa gaaatgaatt ttaaaaaatt
    27901 ttcttttaat tctatttatt taataattgc tgttggtata atttttacag ctattttatt
    27961 tagtatctca atttatctaa taactgatga tttaaaacta attgctctat tattaatata
    28021 tacaatagta ctttgtggat ttataacatt aataatattt ttacttcata aaaaaatagt
    28081 tatattttgt tctaaaatat gtcacacttt agatagtatg atgaatagtg aagacaatat
    28141 tgaaattgaa ttagaagaag aaactatttt atcacgtatc aatcacaggt taattagaat
    28201 gtatgaagtt attcaagaaa atcgcaatag tattgctata gagaaagctg atttacaaga
    28261 actagtttct gatatatctc atcaaattaa aactcctatt gcaaatttaa aaatgattaa
    28321 tactactcta ctaaatcaaa catttaaaac agaaattcaa aaggattttt taatggatat
    28381 ggaaaaccaa ttagataaat tagatttttt aatgcaatct atgataaaaa cctccagact
    28441 agagacaggt ataatcacac tatctaaaaa gaaaaattca atttatgaaa caattgctac
    28501 agcacttagt ggaattttgt ttgatgctga aaaaaagaat attgaagtta cagttgattg
    28561 tgacccaaat ttatatataa ttcatgacaa gaaatggact tctgaagcta tttttaatat
    28621 attagataat gctgtaaaat acacaagttc taatggtaag atacgtgtag ctacagaatg
    28681 ttgggttatg tatacaaaaa tagatattat tgataatgga aaaggaatat cagaaagtcg
    28741 ccaagcagaa atctttaaaa gattttatcg tgaagaagat gtccatgaca tagaaggtat
    28801 tggaataggt ctctatcttt caagaaaaat tatatctctt caaggtggct atattaaagt
    28861 tacatcagat attggaaaag ggtcgacttt ttctattttc ttacctaata aataaatttt
    28921 taaatatttc atttgtaatg attctgcaac attttggttt tatactgtat ttacaagatg
    28981 ttaaggaggt attattatga gtattttaca agtaattaat ttaaagaaat attatggaca
    29041 aagttcaaac attacaaaag ctcttgatgg tgtaactcta tctattgaaa aaggcgaatt
    29101 tatttctatt gtaggtacat ctggtagtgg caaatccaca ttattaaata tgatgggagg
    29161 attagataat cctacatctg gaaaggttat tataaagggt aaggaattat ctactatgtc
    29221 tgatgaacag cttacaatct ttagacgcag aaatataggt tttatatttc aaaattataa
    29281 tcttgttcct ttacttaatg tatatgaaaa tattatctta ccaataggat tggatggaga
    29341 gaccatagat aaggatttta taaatgaaat tataaatatg ctttctcttg aagaaaaatt
    29401 atatgatata ccaagtaatc tttctggtgg tcaacagcaa cgtgttgcta tagctagagc
    29461 acttgtaaca aaaccagcta ttatactagc tgatgaacca acaggtaacc ttgatagtag
    29521 aactagttca gatgtactga gtttacttaa agtgacaagt aaaaaattca accaaacaat
    29581 tgtaatgatt acacacaata atgaaattgc acaactaact gatagaatta ttcgtattga
    29641 agatggaaaa attgttaact aggaggtgta gtatatgttt agaaataata atcgcacatt
    29701 tataagaaaa atagctttaa atgatttaaa aattaataaa cttaaaacat atctttcagg
    29761 tatcattata atgatttcta catgtttatt attaacagtg accttagttt catataatgc
    29821 ttctgtagat ttagttaatg catctccata tcatgctata tataaatctg tagatgaaaa
    29881 agcaaaaaat atactatata aaaataaaaa ctttagtggc gtaggaactt acaaattaat
    29941 tggaagtaat aaaaagacag attgtattat gtcgattgtt tatgcagatg atacagcaat
    30001 taaacttatg aattttcaac cattgaaagg caaactacct acaaataaaa atgaaattgc
    30061 tatttcagaa aaatatttac aggaatttgg tttagataaa gatattggag atagcattaa
    30121 attaaattat tatgatgata ttacgaataa agaagttcaa tgtgatttta ttattgttgg
    30181 atttctagaa aattattatc aagataatgc caagcaatac tatacagtcg tttcaaatga
    30241 ttattttaag aatatagctt ctacaccttt acaaaataaa agtaatactt ttgacgagtc
    30301 aagacctgat actgtagatg tattagttaa actatcagaa gaaaatacag agaaagattc
    30361 agcttcaatt aagacacagt taaagaaaat tggtttatct ttaggtatta agaaatataa
    30421 tatatactta aatgataact atattgaatc aaatttaatt gatggtgagc agattattac
    30481 agttttagta gttggaatag ttgttctttt ttctagcgtt tttgttatat atagtatttt
    30541 ttatatttca gttgttaatt cggttcagat gtatgctaag ttgaaatcgt taggtatgac
    30601 ttcttttcag ttaaaaaaga ttatttctct acaaggaaat attttgtcaa ttatttttat
    30661 tccattaggg gttatagctt cttgtataat tgcttacata attcaaccat tagcatggca
    30721 aatgatagct gatttattta taattttaat tttatcactg gtaatgtttt taactgttag
    30781 aatttcttta tttaaaccag ctagaattat ttctaaaata tctgctattg aagccatgca
    30841 atatacagaa actaaattta gaaaaaaagc aaaaaatttt agttatataa atataaaaaa
    30901 tctagcatta aagaatatcg agtcaaatag aaagaaaaat ttgattgcat taatatcgct
    30961 aagcattagt ggagttttat ttatttcaat cgctaatctt gctaattcaa tagatttcaa
    31021 aaaacaacta tctcaacaat ttgtctataa cgaagattat ataattagca ttaagagtga
    31081 taacttatat gagcatatta cagaaatcca acaaaataat ccactaacag attctcttaa
    31141 gaatgaaatt aaatctattc atggagtaaa aaaattaatt gaatccaaat caattaaaag
    31201 taatattata gaaccttaca tcaaagatag agacgaaaaa caatttatta ctctaattaa
    31261 aggtattacg cctgaacttt caaaatcact tgaagaatat attgtcagtg gaaaaataga
    31321 ctatacacat ttagattcag atagtttaat tataaataaa tatagaacaa aatcttatgg
    31381 attaaattta aaagttggag atacagttac atttaatatc tttagtggaa atgatattat
    31441 taaaaagaaa atgaaaatta taggaatcat tgacaattcc aatacaggag gtatgttttt
    31501 tgcttcagac aaaactttag aatctctaac accatataat acaaatttag atttttctat
    31561 tttaatagat aaaaagcatt ctaaagaagt tgaagaaaag ttgttaagta taattcagaa
    31621 aaattcaaat ttaatgcttt attcatatga agatgattat agaatgatta ctagagcatt
    31681 tcaatatata attatagctt catacatttt tgttggttta atttcttgct ttggtatact
    31741 aaatatgacc aataccctaa taaacagcgt tttaattaga aaaaaagagt ttgcattact
    31801 tcaagccgtt ggaatgacaa gaaagcaact gcgaaatatg ctttatagag aaggtctaaa
    31861 cataagtatc aaagctatat gtacttcaag tatactaggt tattttggtt caaatctact
    31921 ttgcactttt ataaaagatg taattagatt agactttatt aattttaaat tttctatatt
    31981 tactatttta attttttcat tcgtattaat tggtatacaa gttttagtga ctgaattact
    32041 tgtaagaaat atagaaaaaa aatcacttac agaacgctta cgttctcagt aagaatgtat
    32101 ataaaaaata gccatttttt atacaagttt taatatgatt ttttaaaagc tatagggata
    32161 gaaaattttg actttacata attttctatc cctatacttg tttttatttt atacagatac
    32221 acttacttat atctccgagc aacatcacca gagacataaa caagcatatt ccttattttc
    32281 taaatatgtc aattgcatga cttgtaaccc ctactaaatc ttcacgttca caagtagcaa
    32341 ggtgatattt taaatataat gcaaatgcat catcatccat agagtctact gcttcacgca
    32401 tatacaaagc caaaccatct gatgcgacat aatgcagtct agttacatta aaaatcatca
    32461 atgttttctt tacgtacaag ctcaaacaaa tcctttggtt cagacttagc agcaaatgtc
    32521 tgtggatcaa tcaaaccttt ttcaatatat tcagatacat caatatttcc tctatggaac
    32581 ccctcgtcaa tgagacagcc atcagatata acatatgcag caaaaaccac accaccaggt
    32641 tttgttacac gaattgcctc atgtaaagct tgttgtttat cttctttgtt ataaagatga
    32701 tatagtggtc ctaacagtaa cgtaatatca tatttattat ctgggaaaac agataaatcc
    32761 atagcattac cctgagtaat gcttatattt tcatttgatt gagtattttt atggaaaacc
    32821 tctatattat gttcgactaa ttccactgcg tcaacatcat atccctgacg tgccagtgtg
    32881 tgagaataac gaccagttgc agcgccaatt tcaagcacac aatcacctga ttttatgtac
    32941 ttatcaatgt aatgcatagt agtgagaaat tccaccattc catgttttaa tgcaagtcga
    33001 ctatcttcat cataagtatt ataaaagtca attaagtatt gttttgtttc cataatttca
    33061 cctcttctat attagttaat aaacatcaaa tagacaaatt ccaatctggt ttttcttgtc
    33121 ttataaaagc tgttaatata tttttcaaca ttttttcagc actatatcta tctaatgtta
    33181 gtttttttgt tgctgttaag acagctatgc catgagtata aagaaataaa tctaaaaata
    33241 ttacttttgc acattccaac tctattccaa tgctttccga aaaaagtctt gccttttttt
    33301 cattgtcaat ttctttataa aaatcattcg cctctttcat catcaaatct aaatcattta
    33361 taaataacaa cttaaataaa tacgtttctt cttgagcaaa ttcaatgtat gaaagcggaa
    33421 gcagtaaata agggcttaca cttttagaat tacgataatt aacaacatac tgattataat
    33481 attcatatgc aaaagcaaga aattcatttt ttaattcatt catgttttca taacaagtaa
    33541 aaattggtcg agtggaacac tgtaatttac ttgcaatact cctcgcattt acagcttcaa
    33601 atcccacttc tcttgtaata tccaagactg tattcaaaat catttctttt gttatcttta
    33661 ctttaggtgg catatatgtt cacctctcca ttaaattttt ataaaacaca tatgttatat
    33721 aactatagta acatatcaag aaattatgtc aagaaaaagc tttgcatttt tttgcaattc
    33781 ctaaaactta taaataaaac atgagttgca attaatctat taatagataa cagcaactca
    33841 gattttcctt taaatactct tctttttgtc taatatttca tatacacaat cttctattga
    33901 attaatatct ttttcttttc tttttttaga atcaattaaa atgaaataca gtataaaacc
    33961 tacagcaagt ccccatgttc cgcctttagc agcaattaca cctcccataa gtccaatcaa
    34021 tcccttctca acacctgtat tacatagctc cattgatagc tttgtacata aatatccttg
    34081 aactaaaagt gttactgaca atgctaatgg tataagaggt gtagcaatct gcgcaattgg
    34141 ataaaagcag ataaaaattg ctgaaaccca tactaatgta cccataccac taaaaacaga
    34201 ctgcatggct tcctttccaa ccttgtacct ttgagcaaca cttcctgtaa gtgtcgcagc
    34261 taatggaccg cacattggta tatatggaga aaatattgcc ataaaaatat ttcttatccc
    34321 acttataaca ttagacctat ttgaattgaa atctatatat tcatcttttc tattttcact
    34381 ttcagaaagt actaagtttt cacctgttat aaaatcacca aaggcaatta tgtatatcat
    34441 aaaagccatt ggtatccctt gtataaataa cattacactt ggaaaaccta ttccaaaagg
    34501 agatactgta tttatcagat ttttaaaatc tactatgtaa actaaattgt caaaactaaa
    34561 tctaggaatt tctatttctt tactaaatat acctactatt aaactcacta aaatagctga
    34621 tactacacca tattctccta aatgaaataa aaatttattc ttaactttta gagtatcaaa
    34681 tctttcagaa aacattacaa aacaagcaac caaaattcct actgttatag atataggata
    34741 agaatcaaat ctccctttag gattaaactc tgatattata gtcgatatac cgacacccat
    34801 tagtattcca cctttaatag aatttggtat cttactaact aactttccag ctacaccagt
    34861 tatacccatt aaaataaata ttattcctaa atctatttgt aaagctgtta atgcttgtat
    34921 tctttcaatg cccatgctga actttaacaa atatgcagtt gtaagagtta tagaagctgt
    34981 aatccagcca ggtacaactg ggtctcccat aaaaacatgc aaacagtata atattgagtt
    35041 aatgatagcc atactccatg ccacttcata gggtaatccc aaaatttgtg tactaaggcc
    35101 aattgtgcct aaacttgtca tacttgatat tataccttga atagcttctg gtttctcaaa
    35161 tctgtaatgt ataaatggaa ttcttatctt aaacattcca aattttatat gaggttgttc
    35221 ccttctagcg agtctttcca taacttcact ccacctttca ataactctta taagtttaca
    35281 actttataat tgtttataaa ttattaatgt cattaaaatt agtatataac atgcaaatta
    35341 tataaacaat aaattaaatc tataaaattg atagattttg tgcatatata gaaagaccgc
    35401 ttcattttta gttacttaaa tcacttaata ttagttattt aaatcacttg ataaaggttt
    35461 tttataaaat tcaatagttc tattccatga ttcaaactgt ccttttgaat ttccttctac
    35521 agttccacct gaagaaaaaa tccctccact catactcata cataaagtct cctttagagg
    35581 cataatatat ggaattggta acggatgacc catattattg tatgttaata attctacgtt
    35641 atatggataa ttattttctt caaagatttt taatgcctct aaacaagctt cataactatt
    35701 ccatatgtta tcttcttttc cagcaatcat aagtatatct gctttggaat tttctatttt
    35761 tattctagcc tcttttctat tctctgctct tttaagactt ttcttataag tactattaaa
    35821 tccaaatgga ataccttttg atatattttt tttctgttct tcaaagaata tatcattgtc
    35881 tactttgata tatggaaatg gctctccttt atatgaccat gatgaagtat catttccact
    35941 catcaatcca ttaagagctt ggaaacaata aatgtgaggt tgtgatacta caaccttttt
    36001 aatttgttca tatcttgaag caagtaaaaa ggccaattct ccaccctttg atgtcccatg
    36061 tacaaatatt tctttagtac tggtaatttc attttcacta atccactcaa atactttttc
    36121 aaaatattct aatggaacct cttctaattt atctggtaac ccttctaaag caaagtatgc
    36181 aactgttagt acattgaatc ctctagatgc aagtggagct gctaaaaggt ctagagcgtc
    36241 taaatttcca tcagaaccac ctagcattaa aattgtttta ttattagaat tttgtttata
    36301 aaataatttt cctataaact catcagatat gtattttgtg gtaatatctt ctgatttaaa
    36361 tattctcttt aattcaactt cttttctttc aatagaagtt tcaaatatca tattcataat
    36421 tattggttta tcaatagata tattttctgc aaaattttta ccttcagttt tagatttttc
    36481 cagcgaataa attaacccca tacaattatc agccttataa gtaccttcta taggttcaac
    36541 taaatctaaa tctacttctc ctttttcatt tgaacaaaac actccataag atgaaaattc
    36601 ttctcccttg caccatggaa attccatttt cacacttatt ttaagtttag tatttggaat
    36661 taagttcgaa accataatct taagtctttc atccacaaat gattctacat tgtatacatt
    36721 tactttcata atctatttct ccttataata attatttatg ttttatataa aacagcttta
    36781 tctaatacaa cataaataaa tttttaaatt aagacaccat attgactatt tcattttatc
    36841 aacacattcc atttttgtca atatattgtc ttttttagta atttaaaatt atgtagtcta
    36901 tatgaaatcc agttgatttt tggctaatta aagataggaa tgctattagg tttagaatat
    36961 atatattcaa aataaaaaac cactcatttt aagaatggtt tttaatataa actatttgtt
    37021 attaataagg atattttttt atttaaattt atataatcga acatctaata tttaatgttt
    37081 aataaaatta tgcatttgca aaagtatata tggcattaac tcttgctaca tataaattct
    37141 catcactaca tccaatatat ctagcatatt tttcaatctt ttcttttgca ttttcaacat
    37201 cattttcatc gctctccatt aactcattta tataaatatc cattaatgat gctttatatt
    37261 ctcttatatc atagcctaaa aattcagtca atctctcaat ttccttacta aaatctccat
    37321 cttcttttac caaaaattcg tatacatctt ccctatatgg atttaaagtt attatttgtt
    37381 ttactacttt gcattcgtca gccttcccaa taaaaccatc tttataatta ttaaataagg
    37441 cattagattt ttttatagat tcttcatcaa tataagaatg taccgcagtt atacgatttt
    37501 ctataagggc atctacaaca gcataatgaa ttttaaaaca atcattgtat aaataattac
    37561 tcattttctt tattgtcgat tctatttcta agaagctcgc gtggccattt tttttatttt
    37621 tatttggtat aataaaagtt ttattcaagt tattaaaaag tctcttaaaa tctaaatatt
    37681 tttcacaata aacactttta aaggtatcga tatctatagt tataattcca aaacttacta
    37741 tagtctcaat tcctttctta aaagactctt ctatatattt ttctcctaaa cttaaacctc
    37801 tatcagatat ttgctttaga ttttgaaaac tatttaaaca atctctctca aagtttttag
    37861 atatttctaa agcatataat ttaaagattt tccttatctt attataatta ttttttgtat
    37921 catcaaatct gataatagct ccatttacat caaaagtaat cattgagttt taacctcccc
    37981 attagtttca tcaataaaat aaatttaata taaactttat aatcataata ttaaacttat
    38041 ttatactata attattaatt tgatgataaa agctattttc cttctaaata aaaaatttgt
    38101 ctaaagtttc taaaaaattt agacaaatct taggttatta ctctttaaat tatttttaaa
    38161 ttattccaaa tacgccaaat aatattggta taaatatagc tggtaacaaa ttggctactt
    38221 ttatattcgt taatttaagt acatttagac caagtcctat aattaataaa cttccagctg
    38281 ctgtcatttc tgtgactaca ggatcactta aaaatccatt taaaaatgat gctcccacac
    38341 atataatacc ttcatacaaa aatacagata ttgcagaaaa cattacacct atacctaaag
    38401 aagcagtaaa tataattgaa gctattccat ctataacaga ttttacaaat aaagtatcat
    38461 gacttcctgt aagaccactt tctaatgaac caactactgc catggctcct atacagaata
    38521 ataaactaga tgatataaaa ccctctgcta tagaatcttt tttatttcct ttagaaaatt
    38581 tactttgtaa aaaagcaccc aacttattta cccatttgtc aatatctatt aattctccta
    38641 tcagagcacc tacagctaca gatattattg tcactaatgt gttttttcct tctagtgctc
    38701 ctgatatacc aatgtacaat acacataagg caattccatt cattatagta tcgcttactc
    38761 tttgaggtaa tcctcctttt attataagac ctactataca tccacctgca attgctagac
    38821 tatttgctat tactccaaac attttttctc ccctcgatta gctaattgaa atttatccta
    38881 ttattaaaac ctaaacatct atactttgta ttggatattt taaatattcc aaaaattctt
    38941 caaaaccttt tctagatata tttacagttt tagtatttat gttaggatga aaatttaagt
    39001 aatcttcttt aattacatct ttatctatgt aaagctcaac ttttctttct gtatcattta
    39061 ttaatgaaaa aggatttaca cttccaggaa gaagtttaag tacattgtat agtctttcag
    39121 gtgaagcaaa cgataatcta gaacttccta tcttatcttt aagtgagttt aaatcaactt
    39181 gtttttctcc tttaactaca ataagataat acttatttcc tttggcattt cttaaaaata
    39241 aatttttaca ttgacacccc tttgttattc catctaaaat gactaattgc tctgctgtat
    39301 atacagcttc atgctctact acatcatatt caacatttaa tttatccaaa atatcatata
    39361 ttttagtttc attcatgcta gattcatttt catcttgttc ttttttatta tttaaatcac
    39421 tcatactttt ctccttaaac acttcatcat aatatcattg tgaggtatta ttctactata
    39481 gctattgctc ttactggagc tccatcagca tcattatact taaatggtgc tgctataaat
    39541 agaaattttt ctggaacatt ttctaaattt gtaagatttt ctatgattat ttttcctttt
    39601 tcaaataaaa tatgatgcac ttcaaatact gatgtgtcat atctatccac tgatagcata
    39661 tctattccta tgccttttat attagtatta gctatatagt ttgcagcttc ttttgttaat
    39721 gtaggataac ctacatagta ctgcttttta tcccaaaatt ttgaccaacc tgatttaaat
    39781 attataaaat cactattttt tatttcatct tcatattttt ttagatattc taattctata
    39841 ttctcatttc ccttttcaag ttccaataca taagcttttc caacaaaatt ttctatatcc
    39901 aacttatcta gggtctcacc tttagtatac atatgtcttg gtgaatccat atgagttcca
    39961 ttgtgagaaa atacactaat taatgtttct tgataaccat tttcttcaat aatagctact
    40021 ttcttgatat ctggtctatt aggctctgca tatacaggca tgtcattatg tgtcacatga
    40081 gttaaatcaa aaaccttcat ctttatatac cccctatgtt tttattttct attatatacc
    40141 taaattcatt ttatttctat cagattttat tccattatat accatactac tatatatttt
    40201 ttataaacct caagggattt accgaaaaat cttggaaaga attatcagta aatataacat
    40261 cttcacccaa aaaacgttta taccttcctt cttctttctt taaagtaatt ctctgttttt
    40321 gtgaatgatt attaaaaacc attatcttaa gaggaggttt ttttctatta gtctgcactc
    40381 tttttaacat gtattttatg tgcatatcag cttcaggaaa tgaatatcca caaaatatta
    40441 aaaggtcagt atctcttaaa gttttttctg tttcattcca aacattactt aaaaatatgt
    40501 ttgacatatt cttaaaatat gttggtggca ctataatagg ttctgttaac tcaccacact
    40561 ctaaacattt tgtctctgaa ctattttcaa taagcttcat gacaccacct tcatgtggtg
    40621 taagagttac actattacaa atggggcaat aaagccaatt caatgaccca tgtatcttat
    40681 ataatttgat taatggttct tttggttttc tccaatttaa ctcctcttta aaatttgtaa
    40741 actccacacc ataatcaagc ataattggca aatcattctt atctcttaat ccagtaactg
    40801 tattgtctat atgtatatca taatttgcac taataaaagt agtatcaaga agcaaatcat
    40861 tttccaaaag attttcaact aaaagtttat gatatttatt atctgttcta ggtgtattat
    40921 gaattgcttc agccattagc agtattaaat attgtcttaa aaaacgtata ctatcacttc
    40981 tgttatttag attttctagt ccaaaatttc taaatgcttc ttttctttgt tctgctaagt
    41041 ctaaaagacc taaaacttct tcaaaggtag gaaaatttgc attatctata ttatctttta
    41101 ctatatcaat attgaacatc tgtttaaaga aaatatacaa ttctctattc attttggtat
    41161 tataatttgt gggatttagt tttttaaaat attcgctaaa aagttcattt tgaataggta
    41221 gattctctgc tgctgaagct cctgctccta aaaatattgc tattttcata tagttcctcc
    41281 taatataaat attactccta tttttatatc cattaaatat ccatatcatt tattttatga
    41341 tacaacaata aagctgtctt ttactagaaa aaatcctatt acaagacagc cttatattat
    41401 attaaatctt taataattcg taaatagaaa gagcatatat ttgagtagct tttataatat
    41461 cctctactat taagtattca ttagcttggt gttctgtttc tggttttcct ggaaaaatag
    41521 ctccaaatgc aacacaatta tctaaagctc ttgcgtatgt tgctcctcca gatgaaagtg
    41581 gtgttccatc aagaccagtt tcttcttcat atacttttct aagtgtctta acaagaagag
    41641 tatcttctgg aacatagatt gaatccaaga aatcatattc ttcataattt aaattgtatt
    41701 tatctgtcat tttctttaac tcttttacaa aatcatcctt tttgtatgtt actggaatcc
    41761 ttacatctat tcctgcaaat tctttttcgt tatcaatagt tactctacct atatttactg
    41821 ttagtttacc tgatacatca tccttgcaat taggaattat attgtttcca ttagcatctt
    41881 caccaataac ttctgctaaa aacttaatta tattagaatc tatacctata ttattaagag
    41941 ctatacaaag tctagccact gcatttatac cagtatcact tgctgctgaa tggacacttt
    42001 ttccaattat acagatttta ttaccttcaa cagtatattc aaaatttaat ttatcaagtt
    42061 ctttttttaa tttatcagag tattttccaa tatagattgc tttacctgga actgcattta
    42121 aagcctttcc aactgacaac tctatatcac ttttgctatc acaagtcaaa tgaacttgta
    42181 aaagaccttt ctctgcattt gttattggga atctagaatc tggagtaaat ccatagtttg
    42241 gtatttcttc attattttct ttatatttat ttatacatct ccaaagattt tcttcatcag
    42301 ccccaaaaat gaatcttatc ctcttattaa agtctacatt taaatccata agagctttca
    42361 ctgcataaat tgctgatatt gtaggtcctt tatcatcttg tgttcctctt ccatacaatt
    42421 taccatcttc taaaacagct tcaaatggag ggtaattcca gctttctaaa tcaccttctg
    42481 gaaccacatc aacgtgacca agtataccta tcattttttc accttgtcct atttctgcat
    42541 atccataata tccatctttg tatactgtct taaatcctaa agatttacat aagtctaata
    42601 tacctcttaa agctttatct acattttcac caaatgggca attttcagtt gcttcagata
    42661 taacactagg tatttttaca ctttcttgta ttgaagaaat catttcatct tgtagagaat
    42721 ttactttttc tttaatttgc tctttcatat tatcaccctt caattttcat cataatatac
    42781 taataaatat tttgaccttt taaaaaaaaa atatgcttat tttttcagtt tttttataaa
    42841 ttcaatcgct tttaaagtcc ccatttttga atggtcaaat gtaagtccac cttgaaaata
    42901 aacattataa ggaggtctta ttggagcatc tgcacttaat tctatagaag aaccttgtat
    42961 aaatgctcct gctgccatta tgacttcact ttcgtatcca ggcatgtccc aaggaacagg
    43021 ttttacataa gaatcgactg gtgcagcttc ttgtatacct tcacaaaaac ttattacttc
    43081 atcagcatta ttaagtctaa tacattgaat aatatcactt cttaagtcat catattttgg
    43141 taatacatca tatcctaatt tttcaaaagc tcttgagcaa aagatagctc ccattacagc
    43201 ctgagatact atataaggcg ccaagaaaaa accctgaagt acatttctag ttgttccaaa
    43261 agtaagacca cattcttttc ctattccagg agaagtcatt ctataagata taagctcaat
    43321 caaatctttt ctaccagcta tatatccacc tgtcaatgca agcccaccac caggattttt
    43381 tattaaagag ccagccataa catctgctcc aacatcagta ggctctttgg tatctaaaaa
    43441 ctcaccataa caattatcaa ccattactat agcttctggt ttaacagact ttattacttc
    43501 tatagcttct tttatatcac taattgaaag tgattttctc caagaatatc cttttgacct
    43561 ttgtatcata acgagtttag ttctatcatt tattttgttt ttaattcctt ctaaatctaa
    43621 gtttccatcc tctaaaaaat ctacatcatc atatgtaaca ccatactctt tcaatgaacc
    43681 tttttcttct cttatgccta taactccctc taaagtatca tatggtctac cagtaacaga
    43741 taaaatttca tctcctggtc taactatacc ttgtatacat aaagtaagtg catgtgtacc
    43801 atttacaatt attggtctta caagagcatc ttctgtatta aatactttag aataaatttc
    43861 ctctattttt tcacgaccaa tatcattata tccatatcct gtagtccagt taaagtgcat
    43921 atcacttagt ttagattctt gcatagcttt taaaacttta tattgattat attctcttat
    43981 ttcttttatt tcttcaaact tatcttttat ttcttccatt atttcttgcg atagtttaaa
    44041 agtatcatca tctattccat agtagtcttt taataattct tttgtctcat taagcatttt
    44101 atcacctgtc tctttattat tttatttaaa catgtaaagg agactatttt actaaaacaa
    44161 ataaattata gtttataagt tagtataagt ctcctctcaa tcatcttaaa ttaaattaat
    44221 tatctatctg ttgttattat tattgttgtt ttggttttga tttgttagat taatatactt
    44281 tccaggttgt atagttgata cagcatgttt ataaatcatg ttttgttgtc tattatcccc
    44341 ttctattaat attatataac tatcaaaccc ctttacaagc cctttaactt gcactccatt
    44401 aactaaatat atagtaacag gtatcctttc tttccttgca ttatttaaaa acaaatcttg
    44461 taaatttaaa actgtatttt tcattagatt tgtaccctcc caagattaaa ttgaacttct
    44521 aaaatattcc taccttatat tctatataag ttatgacaaa ttttcaatat taagttgtat
    44581 acttaaaaat ttatatatta ttttatagaa tctttaatat ataagattat ttcatttttt
    44641 aattcatcta tattttcata ttggtctaaa tcaaaccatt ttgctgtttt atatctttta
    44701 aaccatgtta tctgacgttt tgcatacctt ctagaatctc ttttaattat ttcaatagct
    44761 tcttcgtatg tatattctcc atccagatac tttaatatct ctttataacc tataccttgc
    44821 attgatatca tatctttttt aaatcccata cttaaaagtt tttttacctc ttcaaccaat
    44881 ccatttttaa gcataatatc aactctcatg tttattcttt gatacaaatg ttctctatct
    44941 ctatttagaa ctattattat tggttgatat tcttcattaa attttaaatc acttgaaaaa
    45001 tcattcattt tcttaccact tagacaaact tcaagagctc ttatgaccct tttagtattg
    45061 tttttatgga ttctattagc agattcttca tcaagttctt ttaacttatt atgcatgtaa
    45121 tctattccct tttcagcaag ttgtttttga agttcttctc ttaattcatt attggcatca
    45181 gattgtgcaa aatccatatt atatattaaa gagtttaaat aaagtcctgt gcctccagtt
    45241 actaaaacac attttccctt ttcatttatt tcatgtatat atttttttgc tctttgttga
    45301 aactctgaaa ctgaaaatgg atagtcaggc ttaacttcat ctataaggta atgcttcact
    45361 ccttgcatct cttcctctgt aacttttgca cttccaatat ccatatattc atatatttgc
    45421 atagaatcag ctgaaattat ctctgcattc atatcttttg ctaattttat tgacaaatct
    45481 gtttttccaa cagcagttgg acctgtaagt attataagtg gtattttttt cataacattc
    45541 ctttccatat attttaaata tccaaatatt ttacataatt cttttaaaca ttttttctat
    45601 ttctgtctta gatatttcga ccattatagg tcttccatga ggacatgtaa atggattctc
    45661 acacttttct agctgttcca ataaactttt tatttcaata tcgtatattt tatcatttgc
    45721 ttttatagca gacctacatg ccatagatgc aaatctctct ccttttaaat cataattact
    45781 tgtaatttct tctatattat caattatttg aagtataaat ttctcagttt caggtactcc
    45841 aaatatagta ggaacacatc ttaccattat atgattattg ccaaaaattt ctagctcaaa
    45901 accaaatttc ataaataact ccaagttatt ttcaatttga agcatatcta catttgatac
    45961 ttcaataacc actgggtcta aaagtatttg catatttata tcttgtctat agaacttctc
    46021 catatatctt tcatatagta ttctttcatg tgcagcatgt tggtccaaca aatacataga
    46081 atcatctttg ctcaaaatta tataagtatt aaatacaact ccaattacag aatatccgta
    46141 taaagaaaac ttgcgttttg aactacacaa gtattcttct tcactgtcct taatactatc
    46201 tcctaagtaa taatttcctt catttttaat tccatcaact tgaaactctt cttgtatatt
    46261 atcatttgca ctatcttcac ttagataatt aatgtttttt acatcatctt ctacacttgc
    46321 atttaggact tcacttactg attgaaattc atttattggt ttttcacttg ataaagtaat
    46381 aacttcaatg acttcatcct tacctttagt aatattacta tttttaggag tagattctaa
    46441 taaatcatta tttcttaatt tataatctgt acttttttct ctactgttaa tagcgattct
    46501 aggttgtgtt tttttatcac tataagtagc atattttcca ataagatttg agtgtattaa
    46561 tttaaccttt aaaaaatctc taagttctat atacacttct tgttcttttt caaatttaat
    46621 ttcaagttta ttggggtgta tatttacatc tatacatgat gggtctactt ctatatttaa
    46681 gaagcacact gcatgtttac ctattggaat tatagactta taactttctg ttattgcatc
    46741 tatgattatt ttacttttta caaatctttt atttatatag atatgttgta agtttttatt
    46801 tgaacggtat atattgttat tacctatata tccattcatt ttaaagtgat tgcacttaaa
    46861 ctctacatct attatatttt ccgttatttc ttttccatat atacttctaa tagtgtttac
    46921 aagttttcca tcccctggag tatttagcat ctgtttattg ttatttgtat atttaaattg
    46981 aatgtttgga ttacctattg caagtttatt tatcaaatca cttatattta tagtttctgc
    47041 atgagtagat tttaaaaatt tctgtcttgc tggtgtatta aagaaaatat cttttataat
    47101 gatggtagtt ccattagtag aacctattgg ttctttcgat attatttttc caccttcaac
    47161 atatatttta gttcctatta tttcatcttt agttttagtc gtcatttcta attttgatac
    47221 agctgaaata gatgccaatg cttctcctct aaaaccaaga gagtataaat catacaaatc
    47281 atcaattttt ttaattttac tagtagcatg tctcaaaaat gatttttcaa cttcacttga
    47341 aggtattcct attccattat ctgtaatctt aattagactt tttcctccat caattatatc
    47401 tatggaaatt ttatttgcac ctgcatcaat ggagttttca ataagttctt ttactacaga
    47461 agaaggtctt tctacaactt cacctgctgc tatcttattt atagtcaaat catctaggat
    47521 gttaattata tttttcattt ttacacctct aagattttat ttcttttgct tttctttgaa
    47581 gattataaag agagtttata gcatctaatg gagtcatatt taaaatatcc agatttaaaa
    47641 tttcttctga tagtatatct ctatttagac tatcaaaaga gatttgagtt aaggctactt
    47701 cttttacaga atcctcttga ttaattttct ctttatcatc attttgcttc attaaagcaa
    47761 cttcatcatt taatgcttta aatttagaat ttaatttatc ataatccttt tttatatgtt
    47821 tgtagtcttt tactataagt tcatgttcta tttttagaga ttcatattta ttcttaaaat
    47881 tactttgatt taccttaact aattcttcgt ccaaattact attaattata ttattgtttt
    47941 ctttatctcc atttattgca acactgttgt aaacatgatt tttttccaag tctttaagta
    48001 tatattttgc tctttctatg acttcgtctg gtaatttagc taatttagca acatatattc
    48061 cataactctt gtctgctccc tgaggaatta tttttcttaa aaatattatt ccttccccat
    48121 cttctttgac tgctattgaa taatttttaa cctctttaaa ctcttcttct aaatcagtaa
    48181 gttcatgata atgtgttgca aataatgtct tacatctaat atttttttga atatattcca
    48241 ctattgacca tgctaaactt atcccatcat aggtactagt tccacgacct atttcatcta
    48301 atataactaa acttctctct gttgcatttt ttaatataag ggatacttcg ttcatctcta
    48361 ccataaaagt agattgacct tgagataaat cgtcactagc tccaaccctt gtaaatattc
    48421 tatctaatat tggaatatca gcagactctg ctggtacaaa tgagcctata tgtgccataa
    48481 gagctattat agcagtttgt ctcatatatg ttgatttacc tgacatatta ggaccagtta
    48541 ttatgttaat aatgttttct ccacgattta aataagtatc atttggaacg aaattttcct
    48601 ctcctactat attttcgaca acaggatgtc taccatttct tatatcaagt ttattatttt
    48661 catttattgc tggttttaca tagttattta tatgtgcaac tgttgcaagg gacacaaaaa
    48721 catctatatt agctatagtc ttagccactt tttgtattct atctatgttt ttatatattg
    48781 tatctcttat ctctacaaat atctcgtatt ctaaagattt aattttttcc tctgcatgta
    48841 gtatcttttc ttctatttcc tttaattctg gagttatata tctttctgca ttactaagtg
    48901 tttgctttct tatatatgtc tcatctagct ttgcctgttt aaagttagct tttgttattt
    48961 ctatataata tccaaaaact ttattaaatc ctatttttaa tgatttaact cctgtttttt
    49021 ctctttccct gttttctatt tcctttacaa gaaaggctcc attcttagaa atctctctta
    49081 gttccttaag ttcatcacta aaatctgatt taattatgtt tccatctttt atggttatcg
    49141 taggttcttc tagaatagct tcatctatta aattgtatat atcatctaat ttatccattt
    49201 ctgaaacata cttttttagg atttttgcat ttgataaatt tatagtatct tttaaattag
    49261 gtaacttttc tatcgaattt tttaagtgaa ttaactcttt tggtgttact ctctcaaatg
    49321 ctatctttcc acatattctt tctatatcgt atatattttt aagtatatca tttaggtctt
    49381 ctcttaatat aaagtcatct tttatttctt ctattacatc taatctattt tcaattttag
    49441 atttatttat caaaggttct tctacatatt ttcttaaaag tctacctccc attgctgtag
    49501 aagttttatc aagtacgtgt aataatgaac cttttttctt attccctctt atagtttgag
    49561 taagttctaa attagttcta gtaaacatat ctaaaaccat atattctgat gaattgtata
    49621 tatttatatt attaatgtta gatgttattt gtttttgagt attgtatata taattcagaa
    49681 gaattgataa gcttgacttt attagacctt tatcatcaaa ctttagtttt tgtagataaa
    49741 catctggaaa atattctttt aatatattta tatccaaata gttatcacta aaactttcat
    49801 ttatgtatat attacttaca gtagctatat ctctaagctt ttctataaag tctaaatcat
    49861 ttataattat ttcagttgga tggatttttg caatttcttc aattacctta tcttcattta
    49921 aacatgttgc attagtttct cctgtactta tatcaacata agttagacct atattagtac
    49981 catccttata caaagataat aaataattat tctttttatt ttcaagtaaa tttccatcta
    50041 aaactgtacc aggagtgatg actcttataa cctctcttct aacaattcct tttgctgtag
    50101 atgggtcttc catttgttca cctattgcta ctttatatcc attttcaact aattttgata
    50161 tatatgaatt ggctgagtgg aaaggaacac cacacatagg agcacgttct tctaaaccac
    50221 aagcttttcc tgtcaatgct atttcaagag cttttgaagc tactaatgca tcttcaaaaa
    50281 acatttcata aaaatctcca agtctaaaaa acagtataca gtctttatat ctatttttta
    50341 cttcaagata ctgtttcatc ataggagtta atttattcat atctatttgc attatcaaac
    50401 ctccttcttt tctaattaaa tatta
//
LOCUS       DAEMIK010000007        49259 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEMIK010000007 DAEMIK010000000
VERSION     DAEMIK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.007"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..188)
                     /locus_tag="KPK87_000348"
     rRNA            complement(<1..188)
                     /locus_tag="KPK87_000348"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(473..667)
                     /locus_tag="KPK87_000349"
     CDS             complement(473..667)
                     /locus_tag="KPK87_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536548.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(976..2505)
                     /gene="lysS"
                     /locus_tag="KPK87_000350"
     CDS             complement(976..2505)
                     /gene="lysS"
                     /locus_tag="KPK87_000350"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG2536549.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2520..2999)
                     /gene="greA"
                     /locus_tag="KPK87_000351"
     CDS             complement(2520..2999)
                     /gene="greA"
                     /locus_tag="KPK87_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG2536550.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(3108..4076)
                     /gene="dusB"
                     /locus_tag="KPK87_000352"
     CDS             complement(3108..4076)
                     /gene="dusB"
                     /locus_tag="KPK87_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG2536551.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(4213..4983)
                     /locus_tag="KPK87_000353"
     CDS             complement(4213..4983)
                     /locus_tag="KPK87_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG2536552.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(5002..5592)
                     /locus_tag="KPK87_000354"
     CDS             complement(5002..5592)
                     /locus_tag="KPK87_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2536553.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5776..6744)
                     /locus_tag="KPK87_000355"
     CDS             complement(5776..6744)
                     /locus_tag="KPK87_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG2536554.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6737..7714)
                     /locus_tag="KPK87_000356"
     CDS             complement(6737..7714)
                     /locus_tag="KPK87_000356"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG2536555.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(8029..9999)
                     /gene="ftsH"
                     /locus_tag="KPK87_000357"
     CDS             complement(8029..9999)
                     /gene="ftsH"
                     /locus_tag="KPK87_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG2536556.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(10033..11421)
                     /gene="tilS"
                     /locus_tag="KPK87_000358"
     CDS             complement(10033..11421)
                     /gene="tilS"
                     /locus_tag="KPK87_000358"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG2536557.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11604..12032)
                     /locus_tag="KPK87_000359"
     CDS             complement(11604..12032)
                     /locus_tag="KPK87_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG2536558.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(12049..12855)
                     /locus_tag="KPK87_000360"
     CDS             complement(12049..12855)
                     /locus_tag="KPK87_000360"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG2536559.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(13057..13542)
                     /locus_tag="KPK87_000361"
     CDS             complement(13057..13542)
                     /locus_tag="KPK87_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG2536560.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13593..14252)
                     /gene="spoIIR"
                     /locus_tag="KPK87_000362"
     CDS             complement(13593..14252)
                     /gene="spoIIR"
                     /locus_tag="KPK87_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG2536561.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14397..15077)
                     /locus_tag="KPK87_000363"
     CDS             complement(14397..15077)
                     /locus_tag="KPK87_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2536562.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(15061..15951)
                     /locus_tag="KPK87_000364"
     CDS             complement(15061..15951)
                     /locus_tag="KPK87_000364"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG2536563.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(16069..17619)
                     /locus_tag="KPK87_000365"
     CDS             complement(16069..17619)
                     /locus_tag="KPK87_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG2536564.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17713..17979)
                     /locus_tag="KPK87_000366"
     CDS             complement(17713..17979)
                     /locus_tag="KPK87_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG2536565.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(18127..18987)
                     /gene="yabG"
                     /locus_tag="KPK87_000367"
     CDS             complement(18127..18987)
                     /gene="yabG"
                     /locus_tag="KPK87_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG2536566.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(19071..20009)
                     /locus_tag="KPK87_000368"
     CDS             complement(19071..20009)
                     /locus_tag="KPK87_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG2536567.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(20119..20280)
                     /locus_tag="KPK87_000369"
     CDS             complement(20119..20280)
                     /locus_tag="KPK87_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536568.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(20290..20787)
                     /locus_tag="KPK87_000370"
     CDS             complement(20290..20787)
                     /locus_tag="KPK87_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG2536569.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20902..21612)
                     /locus_tag="KPK87_000371"
     CDS             complement(20902..21612)
                     /locus_tag="KPK87_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG2536570.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21788..22318)
                     /locus_tag="KPK87_000372"
     CDS             complement(21788..22318)
                     /locus_tag="KPK87_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536571.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKPIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSDKDERRVKAKANKMVMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(22335..22565)
                     /locus_tag="KPK87_000373"
     CDS             complement(22335..22565)
                     /locus_tag="KPK87_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536572.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22580..23974)
                     /locus_tag="KPK87_000374"
     CDS             complement(22580..23974)
                     /locus_tag="KPK87_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG2536573.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(24100..25353)
                     /locus_tag="KPK87_000375"
     CDS             complement(24100..25353)
                     /locus_tag="KPK87_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2536574.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTATYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25404..26558)
                     /locus_tag="KPK87_000376"
     CDS             complement(25404..26558)
                     /locus_tag="KPK87_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2536575.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(27117..27686)
                     /locus_tag="KPK87_000377"
     CDS             complement(27117..27686)
                     /locus_tag="KPK87_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2536576.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27889..28116
                     /locus_tag="KPK87_000378"
     CDS             27889..28116
                     /locus_tag="KPK87_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2536577.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(28335..29078)
                     /locus_tag="KPK87_000379"
     CDS             complement(28335..29078)
                     /locus_tag="KPK87_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2536578.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            29246..30118
                     /locus_tag="KPK87_000380"
     CDS             29246..30118
                     /locus_tag="KPK87_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2536579.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            30118..30783
                     /locus_tag="KPK87_000381"
     CDS             30118..30783
                     /locus_tag="KPK87_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2536580.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30804..31661
                     /locus_tag="KPK87_000382"
     CDS             30804..31661
                     /locus_tag="KPK87_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2536581.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31817..34363)
                     /locus_tag="KPK87_000383"
     CDS             complement(31817..34363)
                     /locus_tag="KPK87_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2536582.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIVKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34363..35049)
                     /locus_tag="KPK87_000384"
     CDS             complement(34363..35049)
                     /locus_tag="KPK87_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536583.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(35132..36121)
                     /locus_tag="KPK87_000385"
     CDS             complement(35132..36121)
                     /locus_tag="KPK87_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2536584.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(36108..36794)
                     /locus_tag="KPK87_000386"
     CDS             complement(36108..36794)
                     /locus_tag="KPK87_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536585.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(37294..40500)
                     /gene="carB"
                     /locus_tag="KPK87_000387"
     CDS             complement(37294..40500)
                     /gene="carB"
                     /locus_tag="KPK87_000387"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG2536586.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40693..41742)
                     /gene="carA"
                     /locus_tag="KPK87_000388"
     CDS             complement(40693..41742)
                     /gene="carA"
                     /locus_tag="KPK87_000388"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG2536587.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(42000..45206)
                     /gene="carB"
                     /locus_tag="KPK87_000389"
     CDS             complement(42000..45206)
                     /gene="carB"
                     /locus_tag="KPK87_000389"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG2536588.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGIGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(45259..46302)
                     /gene="carA"
                     /locus_tag="KPK87_000390"
     CDS             complement(45259..46302)
                     /gene="carA"
                     /locus_tag="KPK87_000390"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG2536589.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(46328..47044)
                     /gene="pyrF"
                     /locus_tag="KPK87_000391"
     CDS             complement(46328..47044)
                     /gene="pyrF"
                     /locus_tag="KPK87_000391"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG2536590.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47420..48055)
                     /locus_tag="KPK87_000392"
     CDS             complement(47420..48055)
                     /locus_tag="KPK87_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG2536591.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAGGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(48079..48993)
                     /locus_tag="KPK87_000393"
     CDS             complement(48079..48993)
                     /locus_tag="KPK87_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536592.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
BASE COUNT        15333 a         9296 c         5425 g        19205 t
ORIGIN      
        1 aagagatgcc tctcaaatat attatcccgt attagcatac ctttcggtat gttatccgtg
       61 tgtacagggt aggttaccca cgcgttactc acccgtccgc cgctctttac cgaagtaaat
      121 cgctcaactt gcatgtgtta ggcacgccgc cagcgttcat cctgagccag gatcaaactc
      181 tcaaataaaa gtttatcagg ctcagatact attgttatca aaatatctgg ctttatggtt
      241 tgtttcttgt tttataaatt aacctactgt ttaattttca aagttcattt tttatgtttg
      301 cccttttttt aaggacaagt aatactatat catcttaaaa atgtttcgtc aatacttttt
      361 ttgtttttta ttttaaaaaa ggttataact tttggttttt ttaataaata taattaaaac
      421 taatagatat aacccttgat attacaaaca atacatgcat atataatttt acctattttg
      481 caattttatt ttcataagaa tatgaattag ctctcactct atctgcccaa acatttatac
      541 tcttaacttt attaacatta gttgtaccct caaaaatatc cattgcatcc ttacaaaaat
      601 atatcataac atcaagaacc aaaaatccaa cacaaaataa acataaaaaa ttaattactt
      661 gaaacatcag acattccccc aataattttt tatatatagt attctcataa attattcttt
      721 ttaccttctc ataaattatt ctaaatattt tcataataaa ttacttttaa tcatattttc
      781 taaatataaa gtctcaatca acaaaaacta atatcaattt ctaacgtttg ttggtaattt
      841 ataaattaaa tatctaaaag aatctttgaa gcatataaaa agcattccac actgacattt
      901 taaataaaat ttagctattt attgataatt taatagctaa actttattta aaacttagta
      961 gaatgctaat atttcttagt tttcttcttc tttatttgga ttgttatcaa taggtttcat
     1021 agtcgggaat aataatacat ctcttataga tggagagtta gttaataaca taattactct
     1081 atctatacct attcctagtc ctcctgtagg aggtagacct acctctaatg cttttaagaa
     1141 atcttcatcc atttcaaaag cttcatcatc acctaattct ctttttctca attggtctat
     1201 aaatcttcct ttttggtcaa tagggtcatt aagttcagaa aatgcattag ctaactccca
     1261 cttattagca aatgcctcaa atctatcagt tcttcttggg tcttcaacat ttctcttaga
     1321 taatggagat acttcaactg gatggtgagt tataaatgtt ggttggatta atttatcttc
     1381 tccaaattct tcaaagaaag catttattac ttctcctctt ctcattccag gttttatctc
     1441 tatacctttt tctctagcaa cttctagtgc ctcttcatct gtatttatag tagaaaaatc
     1501 tacaccagaa tattctttaa cacaatcttc catactcatt cttttccaag gtggtgtaaa
     1561 gtttatttct gttccttgat agttaactat catacttcct gtagcaactt cagccatatg
     1621 tgcaataaca ttttctgtta tttccatcat atcagtatag tctgcatatg cttgatataa
     1681 ttctattgca gtatattcag gattatgttt taaatccata ccttcatttc taaacattct
     1741 tcccatttca taaactttat caaatccacc aacaataagt ctttttaaat acaattcatt
     1801 cgctattctt aaatacattg gtatatgtaa agtattatga ttagttataa atggtctagc
     1861 atttgcccca cctgctatag tatttaatat tggagtctca acttctaaaa atcctctatc
     1921 atctaagtat gctcttaatg cttttaatgc ttttgttcta attaaaaaag cattttttac
     1981 ttctggattt actattaagt caacatatct ttgtctatat cttagttctt ggtcttttaa
     2041 tccatgatat ttttctggta aaacttgtaa agatttacat agtaatacta cagatttagc
     2101 ctttacagat atttcacctt ttttagtttt aaagatttct ccttcaattc caactatatc
     2161 tccaatatca tatgttgaaa atacactata ttcttcctct cctatagcat ctagtcttac
     2221 ataagattgg attcttccct cttggtcttg aatatctata aagccagctt tcccttgtat
     2281 tctcttgctc attatacgtc ctgctatttt tgtagtttta ccctcaagag aatcaaagtt
     2341 atcttttata tccatagaat aatgagttct atcatatcta ctttctttaa aaggatctct
     2401 acctgactct tgtaattttt ttagtttatc tcttcttact tgaagtactt cactaaggtc
     2461 ttcttgaatt tgcgcttctt cattgctttg ttgattattt ttcatttttt tcctccaaat
     2521 tatcttctta tttctaatat ttcaaactta gcaactccat caggaacttg cacttccaca
     2581 acttctttcg cagctctacc taaaagtgct ttccctacag gagattcgtt tgatatttta
     2641 ccatcatatg ggtctgcttc tgtcgaacca actatagtat attcaacctc ttcatcaaat
     2701 tctaaatcct taactttaac tattgatcct attgtaacaa cattaaggtc tattttgctt
     2761 tcatctatga taacagcttt tctaaccata ttttctaatt ttaaaattct ttcttcaact
     2821 tgtgcttgtt cctttttagc ttcatcatac tcagcatttt cagataaatc accaaaagat
     2881 atagcaactt ttattctctc tgcaacttct tttctcttta caacctttaa gttttctaat
     2941 tcttcttcta atttatcata tccctcttgt gtcaataaaa actctttatt ttcttccatt
     3001 ataaatacaa ctccttcaca aatttcttag gaattttcct aattatattt taaaaatttt
     3061 ataatctttt tttaatttat gcgctatatt ataagtcact attattttta tgtcaaggag
     3121 tcttccatat agtactccaa tttcgacaca acttcttgat agtcttctat tttgttaatt
     3181 tcatccctta attttgcaga gtttctaagt ccttttaaat accaagcgat gtgttttctc
     3241 atttccctta cagctacata ctctccatgt tcttctactg caagttttaa atgcttaatg
     3301 gcagtactta ttttttcgtt aagagttggc tctggcaaaa tctcgcctgt attcatataa
     3361 tggtttattc ttttaaatat ccaagggtta ccttgagcac ccctacctat cattatagca
     3421 tcacaaccag ttttgtctaa catatttatt gaatcttcta ttgtaaatac atcaccattt
     3481 cctataacag gtatactcaa atttttctta atctctgcaa ttatatccca gtctgctttg
     3541 cctgtataaa actgttctct agttcttcca tgtattgcta aagcacttat tccacaatcc
     3601 tctgctatct ttgctatttc aacagcattt acactattat catcccagcc ttttcgaatc
     3661 tttaaagtca ctggcttttt tgagttcttc actactgccc tcaatacctc ttctgcaagc
     3721 ttaggatttt tcataagtgc tgaaccatct ccatttttaa ctacttttgg agctggacac
     3781 cccatattta tatcgagaat ctcatttgaa taatcattca ttatctctgc tgctcttccc
     3841 ataaactcgg gttctgaacc gaatatctga actgctacag gatgttcttc ctcttctatc
     3901 ttaagcattt ttttagtatt ttcatcatca tagcaaagtg ccttaccatt tatcatttct
     3961 gtatataaaa gcccacatcc ctgttcttta caaattaatc taaatggaag gtctgttaca
     4021 cctgccatag gtgatagaaa aactttattt ttcaattcta aatttcctat tttcattgtt
     4081 tttctcctat ttttgccttt attttgtgat ttaactttaa attcttattt cctcattata
     4141 atataccaca tatagctaga aatctatcat aaaaagggaa ttggatatat aacccaattc
     4201 cctgtatatt aattattctt ggtttttttc atatattatt ctcaatcctt ctagtgttaa
     4261 aaaaccatct acataatcta tacttttcgt ctctgaagca atcagtttag ctaatccacc
     4321 tgtagctata accttaacat catcacaatt caattctttc ttcatcatac ttattatttt
     4381 gtcaactaaa ccaacatatc cataaattat tccagattgc atggctgata cagtactctt
     4441 acaaatagtc atacctggct tagctaattc tactctaggt aatttagacg cactttgaaa
     4501 taacgcctca ctagatattt ttattcctgg tgctattgtt ccacccaaat attcaccttt
     4561 ttcagagata gcacaaaatg tagttgctgt tccaaaatca acaagtatac ttggtgctcc
     4621 atacttttct atccctgcta cagcattaac tattctatct gccccaactt gttttggatt
     4681 atcatatttt atatttagac ctgtttttat gcctggacct actattaatg gttgtttttt
     4741 acagtacttt atacaaaagt tttcaagaga atgcataaca ttcggaacta cagatgatat
     4801 tataacatca tcaatatcac ttatattcac attatcataa tcaaataggt tacttatcag
     4861 gattccatat tcatcagacg ttttttccct atctgtttta attctccagt aattaactaa
     4921 tttgtcacct ttatatatac ctaaaaccat attagtattt ccaacatcaa atactagaag
     4981 catatgtcat taaccttctt tctatttttt gcttttctta agtacagtgg caacagcagc
     5041 tactactaaa gcccctacta ttgcttctgg caccccattt gctgcggcaa gagccattat
     5101 tacttttcct gctgaaccac ttttacccat tgcatgaaga tacttgtcag catataagac
     5161 atatatcatt cctaaaactc cagctgtatt taacagagaa cctattgccc ctgtaattaa
     5221 acctgatatg tataccttat ttgtagcctt aattattaac tgatatacat aataagctaa
     5281 tacacctatc ataactcttg gtaatattga aactaatgga tttataaaaa caaaagatgt
     5341 tactgttggg tttgttatag catttaagag acttgatatc ccaaatataa atcctattgc
     5401 tgccccaact attggtcctt ccataatagc tccaataata actggtatgt gcattatagt
     5461 tgcttttgta ggtccaactg gaataaatcc caatggagtc atagatagca taatagaaat
     5521 agctgatagt actcctatta tagtcatctt tcttacattt acttttctta ctgatgcact
     5581 tgtgttcatc ataaaaaata cacctccgtt ccagctcatt aagatgccgg actacttaat
     5641 tttttaattt ttttcgtttt tttcttataa ctatgagtca agctaccatt gtaggatgcc
     5701 cgctctaaca agaataattt tatcaagatg agttgccatt gtcaacaatt tgacaagaaa
     5761 aatatgctaa tttcatcata tttctcgttt ttttactata tctttatatg ccattatatt
     5821 ttctacacta tccgcttttt ttactgcatt tattatactt ttttccacaa cctctacagc
     5881 caatgaacct actaatgtta tatctgcttc tatttctcct gttgatattg tgaatattgt
     5941 atctccatca tgaggtgtat ggataggaaa aatagactta gcatatccat catgtcccat
     6001 ttgagatatc tttttacatc cagctttggt taacttagca tttgttgcaa ttatcccaat
     6061 tgtagtatta tctacactaa atccaccttt actttcacct tgtttcatta atttatatgt
     6121 atcaattgtt tctgtctttt tatcatttaa agcaccagct ataaccttac cattttcata
     6181 aacatctcca agagcattta cagcaattaa tgcagataca actagaccat tatctaattt
     6241 tatagaatag ctacctattc cgcctttcat agcatattcc tgacctctta tctttccaac
     6301 agtggctcca caacctgctc cataatttcc ttgatttaaa atatcagtat ttgcaacttc
     6361 acaagctcta taacccatct ttaaatctgg tctacattta aagctcccta ctcctaaatc
     6421 aaataaaact gctcctacaa ctataggcac tttacctaca cctacatcaa agccaattcc
     6481 tttttcttct aagtatctac ttacaccaca agtggattca agaccatatg cagaacctcc
     6541 agccaaaact actgcatgaa ctttttgaac catatttaca gggtctaata aatcggtttc
     6601 tctagtccct ggagcagagc cccttacatc aacaccgcaa gtagctcctt cttcacatat
     6661 tactactgtg cacccagtaa gaccatttct atcctcaacc tgcccaacct ttaagccttc
     6721 tatatccaaa atattattat acatatcctt tttctcctct tatagaaact tcacctgaaa
     6781 taatttcttt tatacttcca tcacttcttt gaactaataa atttccctct tcatttatgt
     6841 ctaaacattt cactagttct ctattatctc cttttaatac atatatattt ttatctataa
     6901 ttgcagaata atctctacat atttttaagg ttttttcttt ggaattattt acagtataat
     6961 ctaaatataa tttttcaaat tcagtcaata cattcctaac aatatctact ctggataaca
     7021 tatatccttc tttatacaat gatgtggctt tgtcagctat ttcttgttca aaatccatta
     7081 ttttaacatt tattccaatt ccaagaacaa cataatttgt tctttcaatt tcagcagata
     7141 actctgttaa tataccacat attttttttc catttaaaat aacatcattt ggccatttta
     7201 ttaatgtatt tgctcctaga ttgtttaaag ctttcgttac acttgctcct gctattaaag
     7261 ttataaatgg ggctttgaat ggttccatat ctggttttaa tataatactc atccagattc
     7321 cttcatgagg ttttgaatcc catcttcttc ccaatcgtcc tttccccata tcttgatgtt
     7381 cacttaaaat cacagtccca tctacactat ttatagctat ccttttagca tgttcatttg
     7441 ttgaatctat agtgtcaaga tgttcaatat tttttccaat aaactctgta tctaatttat
     7501 agtaaatatt ctgtgggctt aataaatcag ttggattttc cgctaatcga tacccttttt
     7561 tatttacaga ttctatatta tatccctctt cttttaaggc tttcatatgt ttccatatag
     7621 ctgcacgtga cacccctaat tgttttgata attcttctcc tgaaataaat tcagatttat
     7681 tatttaatat aatctctatt attttatctc tcatatttca cctctaatat gacataattt
     7741 catgacttat tttcttctaa taaataatta tacattatta atatactttt aatactatta
     7801 attttaaatt tcctttttaa ttattataat ggttgtttta tattttatat tgatgattta
     7861 tataatttta cattttattt aaataatttt aaaataataa aaaaactgaa agtatcaatt
     7921 aaattaatac atgcaaatat aatttgctta ttaatttaat attatacttt cagttttagt
     7981 atttaatcca catcagataa taatcctaaa atgaccaagg tcttaaaact atttatcttt
     8041 atcagattta tcttctaaat ttttatttat atcaataaca ttgtttcttt cttcttcatc
     8101 tttttctaat tttatagtta attgttcttc aacttcttta gaagaatttt cctctgtaac
     8161 tgcattttca atttcattca gaggtaactc tttattaaat gcttttacaa attgttctgc
     8221 atctaaagtt tcatatataa gtaaagcttg tgctacatac tctaatctat ccatattttc
     8281 ttgtaatata cttcttgtct tatcatatgc accatcaact atacgctttg tttcttggtc
     8341 tatctcaaaa gcaacttctt cagaataatt tctcttactt gaaaaactat tacctaggaa
     8401 tacttcatca tcactgtcaa atgacattgg accaagttta gaactcattc catactttgt
     8461 taccatactt cttgcagtag ctgtaactct ttctaagtca tttgaagcac ctgtagatac
     8521 atccttaagt actagctctt ctgctactct accaccaagt aaaactacaa tattttcttt
     8581 catttcattt tttgtagcat agaacttatc ttcaacagga agttgcattg taaaccctcc
     8641 agctcttcct cttggaacta ttgtaacttg atgaacagga cttacttctt caagtacatg
     8701 ggcacaaact gcatgccctc cttcatggta cgctgtaagt cttctttctt tctcacttat
     8761 tactttcgat tttttagcca cacctgctat aacctttgtt atagcttctt ctatagtttc
     8821 catcttaatt ttcttttctc tttttctagc tgttaatatt gcagcctcat tcattaagtt
     8881 ctctatatca gcaggagtaa atcctggtgt tcttctagcc aagacatcca tttttacatc
     8941 atcacttaat ggtttatttc ttgagtgaac tttaaatata gcctctctac cttttacatc
     9001 tggtgtacca acaacaactt gtctgtcaaa tcttcctggt ctaagtaaag ctgggtcaag
     9061 tatatctggt ctatttgtag ctgccatgat tattatacct tgatttaccc caaatccatc
     9121 catttcaact agaagttgat taagagtttg ctctctttca tcatgacctc caccaagacc
     9181 tgcacctctt tttctaccaa ctgcatcaat ttcatctatg aatatgatag ctggagcact
     9241 tttcttagcc tgttcaaata aatctcttac tcttgaagca ccaaccccaa caaacatctc
     9301 aacgaaatca gaaccactta tactgaaaaa gggaactcct gcttctcctg caactgctct
     9361 tgatagatat gttttaccag ttccaggagg tcctaccata agcattcctt ttggtattct
     9421 tgcacccaat tcaatatatt tcttaggatt ctttaagaaa tctactacct cctgcaaatc
     9481 ttctttttcc tcatctagtc cagctacatc tttaaatgta actctagttt tttcatcatc
     9541 tttatgaacc ttagcttttg atttgccaaa actcataacc tttccgcctc caccttgaga
     9601 ctgattcata aacacgaacc aaataatcac catgaagaat attaacagta atgtaggcaa
     9661 catttcgaca aaccaaggtg tagatggctt agcttcacca ccaaaagtta gtttcccagc
     9721 cttagcttgg tctaacacct catctgcaag tttatttccc atcacttctg ttggaacata
     9781 tgacttaaac tttgtatttg tatcttttat agtaccttct acagaagttt gatttacaaa
     9841 gtataatctt gatatattct catctgttaa ttctctgtat acttcagaga attttaaatc
     9901 ttttattttt tctgtaggct tacctgagaa ttgaactata cctactataa ttataaaaac
     9961 aagtagataa aaacctgccc cttttagcaa cttattcaat atagaagccc tcctttcata
    10021 cattgctttg ccctataatt tgttaaaatt aatttttaat acttcttttg tgttttcatc
    10081 aattttataa ttttcactga ttttataatc tccaacacat attattcctt cactatctgt
    10141 gattatagga attttacatc tttcttctct tgggattttt aaatctatga ataaatcttt
    10201 cactttttta cttcccatag caagctttat cttatcacct tgccttctgc ttcttattac
    10261 tataccccct tttaccttat taaaatcaaa ccctttagat gaattgtcta atttcatact
    10321 cttataccta tctatgctca ttacttgggt ctcaataatt tgttttaatt cttttatttt
    10381 aataaaccca ttactaggta cattgtaata aaattcaatt tcctcattaa caatttcctc
    10441 attcgttaat attatactgt ctttttttct ataaacaaat atatttcttg gaagagttaa
    10501 cattttattt acttttgaat tatcttctaa agacattata tcctctatat gtctttggtc
    10561 aacaaaatta gtatctccta gtatatgttt tatggaattt ctaagaactc taactttgat
    10621 agcatcatgt aagcaaacaa aatcgtctaa atttatctct acaaagcctt tttcttttat
    10681 atttgaaact tctctaaatt tagcttctgc ctctttctca atataatcgt tatcactttt
    10741 taaactattg ctcatcctca ctatagattc tattacattt gaattaaaat tatccttcat
    10801 atatggtaaa aggtctaacc ttatcttatt tctagtgtat atattttcta aatttgtttt
    10861 atctatcctc ggatttaaat tataagcttc acaatactcc tctatctcat ttctttccac
    10921 gtctaatatg ggtctaatta tgcaattatc tctaatataa tcaattccct ttaagccttt
    10981 taaacctgtt cctctcatga tacgcatcat aaccgtttcg gcttggtcat tgagattatg
    11041 acctattgct attttattgg ctttaatctt atccttaatt tcataaaaca tttcatatct
    11101 tagcttcctt gctccttctt ctaatgataa tccctcattt tcacaatatt ttggtacatt
    11161 aatagacttt acaaagaata taattcccat atcctcacaa agtttagata catataatgc
    11221 atctttttga gcttctatcc ctcttatttg atggtttaaa tgtgctgcat atatttctat
    11281 attaaaatct ttttttaatc tatttagtac atgcaataaa caaactgaat caggtcctcc
    11341 agaaagacct aaaactattt tatcgccttt ttgtattaaa ttatgtttat ttatagtact
    11401 tagtacctta tcaaatatca tatttatccc tcttacactt agttttattc acttttaagt
    11461 atactttcta taaaaaaacg ctaaaccctt gtatcaattt taagaaatta aagtttaata
    11521 taaatataat aataccaaat aaattaaata aaaggtaatt ttttcactac ctttataaaa
    11581 tttctggaaa atagccatag ttattattta gtacctatgt atatatctat tttattccta
    11641 cctaaaaata aatcttgtac ctttatatta tagtctattt tcaattttat gtgttcccct
    11701 tcttgtatat ttatatctaa ttctcttgta tcaatatcaa ttaataaagt accttgtggg
    11761 gtcacatact ttgtagtact actttctcca catttaaaca gcattgttga gttaatagca
    11821 ccaaactttt tgatactaac ttgattttta agtatcttta tagtattagt tatttctata
    11881 tcttcttctt tttccttgta aataacatat attccatcat ttttataaaa aattttacca
    11941 aaggcagtca tctctatggt gtccatattt cctttttcat catattgtct agtatttata
    12001 cttattttta cactcaacga tttttcctcc atagattctt catataaact agtatttttg
    12061 tatttctact ttttgaatct tatcaatttt tttctttaag aactctcttg ctatatctga
    12121 aaatttttca ggtatatctg atacataata ttgataagtt ccatgaattt ctgtattgtt
    12181 tataatattt tgcacctcta aaatcttttt taaatccttt gcagtctctt tagctggatt
    12241 aacaagtttt atatgctctc caacttcttc acctattgtt ctctttaaaa taggataatg
    12301 agtacatcct aacactaaag aatctatatt tttttctttc aattcttgta aatatatctt
    12361 tgctgtcagt ttagccactt ctgtatttgc ccatccttct tcaacaatag gcacaaataa
    12421 aggacatgct ttattaacaa tttctatact ctcatctatt ttagatattt ccaagttgta
    12481 tgcttttgaa cttatagttc cttcagttcc tattattcct acaattttgt tttttgtaga
    12541 acttacagct gttctagctc ctgcttctat aactccaatt atcggtatat catatttttc
    12601 ttgagcttct tttaagcttc ttgcagtagc agtgttacat gctataacta tagcttttac
    12661 accttttgtt tttaaaaagt ttatagcttg aaaagtatat ttaattatag tttctttgga
    12721 tcttgaacca tatggtattc tggcagtatc tccaaagtat attatatcct catttggtaa
    12781 tatcttcatt atctctttta gaactgttaa tccacctaat cccgaatcaa aaactcctat
    12841 tggtttattg ttcatcttta tacaacacct actttacatt tttgtgttta acaacaactt
    12901 atttatcgct aaaatttata tttatattca taattaatat attattacct aaatgattat
    12961 tatagtttat tttacacaaa aaataaagga gctatcttaa attagaaaat aatttctaca
    13021 aaaagacaac tcctttcact acttaaaaat ttaaatttat tttgcaaaca catgatttcc
    13081 tattctcata taaggattca atctatttat ccatttgcaa gtggctattt ctggattcca
    13141 aaaatatact gctttattag ttgggtcagt tccataaaga gcttcctgag ctgctttacg
    13201 tgctgagtct gttggtgcca tctttattga accatcaagc acaactgaga aagcattttt
    13261 ttgatatatg acttcttcta ttgtgtctgg aaatctaggg tccaatactc tatttattac
    13321 tacagctgct acagcaacct gtccctcata gctttctcct ctagcttctc ctgcaaccaa
    13381 cttagataat aacatcaagt cattatcaga taaatttatt acttctttct ttgctatatt
    13441 tttttcttgt ttgtttactt tcttaatatt ttctgttggt tcatatgcaa atacttcttt
    13501 aataggcata aatataaaag tcaaagctaa tagtgtagcc atcagtttac tccacctgtc
    13561 catgattaaa accactcctt acattcttat ttttaaaatt ttttctctaa atccttgact
    13621 acttcaagta ctttaaactt aatttttact ctacttgttt tttttggtgt atcttgtttt
    13681 attaacttat attctttatc agttaaaact tccttcaact tatcatctgt agatttatct
    13741 atcactccat tagattcatc aacaaaacat agtggaggga acataacaca ccaccagttt
    13801 tttccttcac ctttcccaat aataatcttt aatgctttat attcaccagc tggaagaact
    13861 atattagaat attgtttagt agggaaattg ctatattgta tacctacctt tacactgtaa
    13921 ttatatccat ttttaagaat aatattttta cttatttctt ccagactact atattccttt
    13981 tttataatag cttcactttc ttttatactt tttgaatttt gaagctttgg ctgtaaatat
    14041 tttattactt catctctaac ttttagttta agttcttggt cttcatctgt attgctgttt
    14101 gctataacat gaaatcttat taatttatct ttataatctt ttgatatatt gtctactttt
    14161 tttacctcac catttattac tattgtcatt attgatatga ctgatataag acttaatatt
    14221 aatagtccca atctaatctt aatttttctc attgaaaatt ccccctcaag ataaatttat
    14281 atcatcaatt atgtccaatt aaatttaatt tattcataat attttttaat actaataaaa
    14341 aaagctatct taaaatgatt acaatcattt taaaatagct ttttaaacct tatattttat
    14401 aagtctttac taatcatagc cttatctatc ataaactgct ctgctttttc tatatgctct
    14461 gtagcatact tcttggatag ctcactatca cctttaatta tagcatcaag taaatattta
    14521 tgctcttcaa caatattatg tgatgtatca taagcagaca tataggtaac tctaaatcta
    14581 tatacttgtt cccacaatga gtttattaat tggattaatt ttttattatt agttgcttta
    14641 aatatacatt catgaaattc tacatcaagt tttaataacg catctacatc ggattccaaa
    14701 gtgctttttt taaattcttc tgatatatct ttaagctttt taatatcttc atcactaatt
    14761 ctttcagcgg caagatatgc tgctaagcct tctaggcttg ctcttacttc taatacatcc
    14821 ataatatcct ttaatgacat atttgccaca tacgcacctt ttctaggcaa cataacgact
    14881 aaaccttcta attcaagctt tcttattgcc tctctcactg gagtcctact tactcctaat
    14941 tgttctgcta gttgcacttc cattagcctt tgaccaggct ttaattttcc ttctaagatt
    15001 gcttctctta aattctcaaa gactacatct cttaatggct tgtaattatc caagttcagt
    15061 ttagttaaat tctccacaaa tttcaacccc tttttgactg ctattaacaa catacacctg
    15121 tttatatttt tttaataatt ctttttttcc aattagagca tcttctttat ttttaaatag
    15181 tccaaaaact gtaggaccac ttccactcat catagaacct aaagcattat ttttcatcat
    15241 tacttgtttt atatcactta tttctttgtg tttacctata gtaacatttt caagaatatt
    15301 taccatgctt tctgatacag cttttatatc ttcagatttt aaacactcta tcaaatattt
    15361 attatttggt ctttttttaa tattattcaa atctaatcct tgataaactt ctttagtaga
    15421 aacgaataag tctggtttgc atatcaatat atttgtatca catggtaaac ctttgatatt
    15481 tgttagcttt tctccaatac cttgagctaa ggcaggtctt cctgatatac aaaaaggaac
    15541 atctgcgccc aaattaagtc ctatttcttt taattcatct tcagataatc taagttccca
    15601 taggtgattt aaacctacta atactgcggc tgcattagaa cttcctccag ccatccctgc
    15661 tgctacagga atattttttt ctatgaatat ttctacacct ttttttatat aaaatttatt
    15721 tttaagtatt ttggcagctt tataaacaat attatcttca tctaaaggaa tatctaaact
    15781 agtgcttttt acctttattt catcttcgtc taattctttt atctttacta tatcgtataa
    15841 atctatcgtt tgcattatca tttcaacaaa atgataaccg tcttgccttt ttcccaagac
    15901 atcaatggat aaatttattt ttgctctact ctttagtctt ataaaatcca ttctatcctc
    15961 cttgtttaaa gaataatgca tcactaacaa taatattata ctacaaaaaa taaaataata
    16021 gggtattata ataccctatt atacttaatt actttaaatt ttttaatatt aatctactaa
    16081 tacgactttt ttttctaaaa taagacactt accaggctta attggttcat caagttttat
    16141 atcattaagt tctgcaattt cattttctgt tgtattgtat ttttttgcta tattccagaa
    16201 agtatctcct tctttgcata tatatacgat tatgctagga gctttagata aatcatatac
    16261 accttggtct tctcccttga taataaaatt ttctgctttc ttatctaatg cttcagtaaa
    16321 tctcttaatt ttgataatca agtctatctg gtccctattc aaatcaactt cgactttatc
    16381 aatacatgct gtattaaaca cagatgctgt atcagttaga ttatccattt ctatatcatg
    16441 ttcaaatgga atttcctcac taattttata aactaatttt aaaccttcaa ctgggacaaa
    16501 taaaatttca acttttatga ttccttgtat aatgctttta tttccttcaa tataactatt
    16561 ttctatagac atggttggac aaacgcttac aatatctttt atttgtatat catcattatc
    16621 atttcttatg ccttctctaa ccataaatgt ttcttcacta cgtcttaaag tcttatttaa
    16681 ttgaataggt ttgtggtcaa attttattat cttttgaggc gaatatgcat cttgtaatac
    16741 ttctcttgta acttcatctg taacttttac cttacaacaa gccatacaat ctatttctaa
    16801 aagacctgtg ttgctttcac tattttgctt gaaaatatga ttaaaatctg acatagacaa
    16861 caatacttct tcagtcatac catcactaac tccagggact tctacaaatt gagtaaactc
    16921 aatacctact ctatccaact ctacaagttc accctcatat gtacaagcta acggatttat
    16981 ttctaatact ccaccaataa taactttatt gtcagtcact ctactttctt ttactttaac
    17041 acatggattt aaacttatta tagattgaat ttcctctgtg tttattgtta tagtatctct
    17101 tatagaactt tctgcttttt ctattccaac tatatcttga aaacaaattt cctttctgtg
    17161 cttttgtatg ccttccactt gcgccacatc ttttacaatg tctaaccttt gcttttcaaa
    17221 caaacttccc cttatattca taagagctcc tactttaatc tttctctcat tcattatagt
    17281 acaatccata tgctctactt ctgaaaataa catgtattcc atatcttgaa taacattgtc
    17341 tttttctatt acttcgttta tatctacttt tccgtctaca ttagaaatag tatttttatc
    17401 atctgcaata tagattacat tataattaaa actacctctg caaagtatct tcccatctgc
    17461 catttcaatt tttttaagtg atatgtatcc ttctgttttt acaatttcat atacatctaa
    17521 ttttttgtct ggcacaactg cctccgcttc aatgaatgtt tgaaattttc caaagtctat
    17581 tctattgtca actttaatta catctttaat taattccata gtctacctcc ttaagtatta
    17641 taaaaatatc acactatatt tatatttaag gagcaaaaaa aaaattacac ctatttttaa
    17701 ataggtataa ttttaactta tttgtaattt atcttgatct ctaaaaactt gcaactttac
    17761 attagatgtt aataaatctg agtaactata agatactcta ggatatccat tactttcatc
    17821 atctaacttt ataacaaaca cgcttggata aactttttct agtataccct cttttgtaat
    17881 aatctgcttt cttcctttat tagctttcaa caatattttc tttcctatat gtctctctaa
    17941 gcttactctt atcttatcta gagtttgaac agtagccaca atatcacctt ctttaacgtt
    18001 tatatgttta cataatatca catataaaca tttttgtcaa atgtttaaac taattatttt
    18061 atatattttt tgcaaaaatg tcaatactat ttttgaagat atacagtata attatgttaa
    18121 tttttgctaa tgtaatattg tttttggcat tgcccatcta aattttcctc ttgtttcaga
    18181 accaccaatt cccttaacac ctgtaatagt ctgctctata acctcatcaa taggaactac
    18241 tacatcaatt cttgaacaag caattttctc cactataaaa acagggtcta atgcatgtat
    18301 cattattctt ccaggtgaac ttgcatagtt tgctcctgca cttattattt tttcataatt
    18361 tgattggcat gctcctgcga atatgacaag attatccaaa tttggttgcc atttacgtgc
    18421 ttcttttact gttttaacaa aatttgaaga gtttctatag ttactcatat cttctatatc
    18481 tcctcttttt attgtcattg catcatgacc tgttattact agtatatccg gattatattt
    18541 ttctagtagt gctcttactt ctttatactg attttgctct gaaacagcta cacctactgc
    18601 aggtattcca agttgagcat atacatccaa gcaaattttt aaatattctt tatctccatc
    18661 tatctgtagt acttttccag gcattccata tgtgttagga tttaattgaa gttttggaac
    18721 tgctctagtc attctttttt gtctttcttt agcttttctt actgacttat ataataaatt
    18781 ttctacattt ttatctatta atatatcttt tatatcaggt gcttttacta attctaggtc
    18841 atcaatataa gcatctgcta ttactctaaa tgcaattccc tttagtatag ctattttctc
    18901 attattttca tctacaccaa aagagactat tttaaatact atatctttgt tatgtgactt
    18961 tctagctaca atgtctccta ccttcatagt taaacctcca ataaattttt atcttagttt
    19021 atactatgtt cgatataaaa ctttgtttcc taatgtcaaa aaaacatttc ttataatact
    19081 tcttttatca ctcttaaagc atttttatag tatatctttt ctatatcatc ttcactaaac
    19141 ccttcttttt ttagaggttc atacagttta cccatctgag atatatcttc aatctcaact
    19201 tttgaatcta ttccatcaaa atctgaccct agagatacaa catctattcc tccaacattt
    19261 acaatatgtt ttatgtgtct aaccatatct tcaagtttac tttcactttt atcacttaaa
    19321 aataagtgga agaagtttat acctgtaact ccacctttat ttgctaatac tttaatcata
    19381 tcatcagtta aatttctaga atgattcatc attgctcttg aattagaatg tgtagcaatt
    19441 attggtttag aagaaatctt tgcaatttca taaaatccac catctgaaat atgagaaaca
    19501 tcaaccaaca ttcccaattc attcatctta tgtaccactt ctcttccaaa atcagtaagc
    19561 cctttttctc tatattctac tttattatga ggaaagctca attcattcac atgattccaa
    19621 cttattgtca tcattctaac ccctaagtcg taaaatttct ttaaattttc taatttacct
    19681 tcaagtactg caccttcctc aatagataaa agagctgtaa gtttaccttc tgattggttt
    19741 ttcataattt cttcatagtt agtagctaaa gctatttcat cactattttt tttcatttcc
    19801 tcatgaaatt tatttgccat actcatgcaa taatcaaaag gatgttttac ttcttcagta
    19861 tcaacaaaaa gagcaaatgt ttgagctaat gaatccccct ttttcaatct gtctatatca
    19921 acagaatatt tattagactt aagttcactt gtttctacat tttccattaa ttttgctata
    19981 gtatcacaat gtaaatcaat aaatttcata tttatacccc ctgtatattt tatatttata
    20041 tctaaaaata gaaatagttg tccttataga caactattga caactatttt ttaatattct
    20101 ttttaatatt acttaatctt attcttcttt taagttaggt attacttttt taacttcttc
    20161 aatttgatgc catgttttat ctatattatt ttcactaata tattttttta aatcagttaa
    20221 agatgtaaaa tctttaactg ataaattgtc atcatcatct gcaaatctta aaataaacat
    20281 gagactccct tactttccac aacatttctt atattttttt ccgcttccac atgggcatga
    20341 atcatttctt cctatttttg cttctttaac tattgtttta gacttcttat aagcctttat
    20401 tatttctttt ctttggtctg ctggtagtat atcttcccaa ccttgtaaat tatatagcca
    20461 atgagcctca acagttacca tattgttata taatttttcc caatctattt ttatagaaac
    20521 ttctttatca gcttctactt cttctaaaac tatatcttct tttaagcttt cacttattcc
    20581 atctatgaat cccatgaaaa attcattagt agttccaaat ttttcagcca attcacttac
    20641 tttaccttca acaacatcca ttttattatt taatatttca ttgtatattc cagcttcaac
    20701 tttcaaatat tcttcccaga attttacttc tgcttcttga ctttcatgat tatcacttaa
    20761 atttctccat tcagtatata aactcatact ttttcctcct aagtatacct atatttttat
    20821 agtttttata gtataaaatc ttttagttag attggaagtt tcttcttttt gattatacca
    20881 tattctattt aaagttaact cttatattat atcataatta aacaattctt ttaatacttt
    20941 tatagcatac tcttggttta taaaataagg gcttgtcgca attgttatta ctttggcttt
    21001 tttaattaag ttttttatct tgtaaaattt gagttcaaat ggtatatagt ccatatcact
    21061 tgaaaatata tccaaatcta tatcaagaac aaactctgat tctacttcta aatcaaatcc
    21121 atatagacta tctattatta ttaattctga aaatatgtta tattttaaag caggttttat
    21181 aaaacttcct acatttaaaa cttcatttgt atatctaaat acatctttta gattatttac
    21241 atctacatca tagttctcag gttctctagt atccttatgt tggtctacat gcactaactt
    21301 gcatcctttt gtaaactcac cctttaacaa agattttgcc caaaaataaa atgcatggtt
    21361 atgattgtca aacacatata tctttttatt tcccaggaca tattcgacca tattctccaa
    21421 tccttttgct tttatttcct tattttcatc tacttcatta aatactacct cattgcctaa
    21481 ccttacatca tttaatgtac catttattag ttttggaacg tatatgctct tattttctct
    21541 tttatcatat gaaaatacat tatttcctac tggtttttct atataaaatc cactatattc
    21601 ataattttcc ataaaacttt atttcctcca ttgttatata aatatataag ttataatttt
    21661 aaaaattaaa atgcacttat tgtaaatcat atcatctatt atagaaaaca tcttatataa
    21721 ctaaatataa tatcttctct caatatgtat tatacaataa gtgcaaaata tatttatata
    21781 aaatatacta cattcctata acaaaaccat cagcttcaat tggaaggtaa ctaactcttc
    21841 tattcaactc cacctttcca aaaaatacta cccaatatgg tctaaaaata ctttcaattt
    21901 taactatttc aatctctggg attcctccca taaacctatg agttaatctc ataaccattt
    21961 tatttgcctt tgcttttact cttcgttcgt ccttatcact atactgcaca atattacaat
    22021 ccacattttt tacaatttca tatgtaggaa aatcttgtac tatactacca gaacctgtag
    22081 aaccattcac taaaattttt aattcattta tatcattaga atatctccta ttcttgaaaa
    22141 tgggtttcgt tttgtgaatt ttaagtgtga taattttaaa ctctatataa tgaagtctaa
    22201 cttcatgagc atatttgttc tttaatatta gcttactaaa taaagaattt tttccacttt
    22261 tagctatctc tatagcttct tcttttgtaa ttcttatagg taacgctctt atatccataa
    22321 aatcattctc tttcttattt ttttcttgtc atttttaaaa ctctatatat ataatcaaaa
    22381 aatattacaa ttacaactga cataactcca tatggaatta tttttccaac tataatatca
    22441 tttctagtca atataccata aaatataaat cctgttatta aaaagtacaa taatactaga
    22501 aaaccgtcta ttccaagcca ttttatttct aaatcttttt ttaccttaga cactaattta
    22561 gacatagcag tttctccttt tatagattat tttgtgtatt taaattttca cctattctat
    22621 taccaatcat ccatgcaata aatgaaaaag gtatagctat cacaagaggc tctaactcaa
    22681 agctctgaac tggaaatatt ttaaaataac caaaagtcac tccaccaaca atcattccta
    22741 aaataccacc catagatgtt cttcttccat ttccaagaag cccacctact ataggtacaa
    22801 atgccgaacc catccaaata cttcctacaa atatacatgc atcatataca ttttcaaatc
    22861 taaatgctac gctaagcccc ataactgctg ctaaacatac acctattcta gtaaacttta
    22921 ctaattgttt ctgagataac tctttttttg taattcttcc atatatatca tttgctaatg
    22981 tagccccagc tgctagataa tagctatcca atgttgatat tatagaacct aaaattccta
    23041 ttataaatat tactcttatt ccttgtggta atgtatctaa aactaaagtt atatatccag
    23101 cagctggagc catttctgga taagtagctc ttactataag tcctgtcata gttaacacca
    23161 tgtcaaaaca tatccatagt gaaaaacaag ttaataaggc tcttcttcca accttaggag
    23221 aatctgctgc tgaaaatctt tgataaaaag ttgggtctga ataagctcca aaagaatata
    23281 caattaacat caatgctttc atagctgaca taccttttgt agggtgcata agctgtggtg
    23341 catctttgag tgcttctgta agaccttgaa accctcctat ttgttcaaat tgacctggca
    23401 taaccatagt taaaccaaat accatacaaa aaaacaatac catatctgta atggcaactc
    23461 ccataagccc accaagtact gtaagtacaa cgactactcc taccattatt aatcctatta
    23521 attctttatc tattttccat gctacgttac ctatagttcc catagcagtt atttctccaa
    23581 gttgtgcaca atatataaac atcaaaattg ccccaattat agcaacatgc ttgccataaa
    23641 aactttccag taaatcagga actgttatat tggttcttat atgaatcctt ggaccaaccc
    23701 acaatgctaa aggcattctc ccaatatgtg cagctattga ccaaactgcc catgcagata
    23761 ttccatatat agctgcatac tctacactac ctactgtacc aactcctcca taccatgatg
    23821 caaccaaagt tcctactatt aaggaccatg gtaaacttcg atttcctagg taataacttt
    23881 ccatatcttt tacatttcta gaaaaataat aacctattcc taataatata aatgcaaata
    23941 taactataat cccattttcc caaaatgtta acatatctaa tctccacatt cttatatttt
    24001 aatttttaac tttaatatct aaatatgaac aactctaccc atttccataa aaataggtag
    24061 agttatataa taattataag tatttaagta atcttgttat taagcaattt tattgtttaa
    24121 acctttattt tctcttttat ttattttatt taaagcataa gtagcaaata tccctataac
    24181 tgcacatact acagataaaa taaatatata tgtatatcct gtttgtccat atttatctaa
    24241 ccaattacct gcaatagtgt aataaaacgc atctggagaa aatcctataa atgaagcaaa
    24301 tccaactact gctcctgtag tttctaaaga tatattagac tctgaaacag atgcaaagta
    24361 tatacctcta aatccaaata aaattacact tagtattatc atgtttatta cagctatcca
    24421 tataaatcct gtactcttag gaataaccaa gtaagtagct gtacttactg tcattaatat
    24481 aaatcctaca aacattactc ttatagatga accaacttta tctgttataa caccagcaac
    24541 tggagatgct cccattttta taacataagt tcttattatt gataaagcag aaactagagt
    24601 cattggcatt acataaactt ctgataagta tggacttaaa taagttaaac ttgagaatac
    24661 taaataagtt gaaaatatta taagaccaat taaccaagcc tttggcattg taactacttt
    24721 taacaatgac ttaatactta atccttcatc tgatgctcca ctatcttttt tctcttctac
    24781 taagaaaaat agaagtatac cacatattat agatgctgca gaatataacc atactacata
    24841 tttgaatcct actgttattt cagcaaattt accaaagaaa taaagaccta taaaagaaat
    24901 taatgtacct gatacgcctg ataacccttc ataaaaacca aatattcttc cttgctctga
    24961 ctcacttcct tgcattctaa caaccttaac agaggctgag aagaaggtta atattgtcgt
    25021 aaatccccat aatacaaata ttaaaagtat catactatat gatgggaatg tagagaaata
    25081 aaatccaagt attcctgaag caattaaaga aaaagttatt aattttctaa ctgagaatct
    25141 atctgcaaac catcctccta taaaataaga taccattcct actaatccat ataaactcaa
    25201 taagttccct atttgtgtat gacttaatgc taaagcttgt tgcattgggt cataaaatga
    25261 tgatttcata tatggtaaac tatacatagc tgttgttcca aatgccaata tgaatattat
    25321 caaaaatctt ttaacattat ttttgctttc cattgattct ctctccttaa aattttattg
    25381 tttataatct cattcatata attttatttt aatttttcta ttgcttgttt aaaatcattt
    25441 attatatcat ttgaagattc taatcctaca gctattctta ttagattttc cccattcatt
    25501 tgaactaatg tatgctcttc ccctaagctt gttgctattg ttgcaagctc taaagaattc
    25561 attaatcttc tactagcttg gtctcttgtc aatccattga taccatcctt aagtttaaat
    25621 gaaactacac ctccacctaa accattcatt tgagttttac aaagctcata ttgaggatga
    25681 gattttaatg ctggatactt taccaattcc acattctctt cttcctctaa aaattctgct
    25741 actgctaatc cattttcaca atgctttttc atcctcatat ctaacgtttt catacccctt
    25801 aaaatcatcc atgcactcat tggtggtaaa gatgctcctg taaaacaaca taaacttggc
    25861 catctaacta aatctattaa ttcttttgaa cctgctactg ctccccctaa agtatcccca
    25921 tgtccattta taaacttagt aagactatgt attactaaat ctgcacctaa atctattggt
    25981 ctttgtacaa ttggtgtccc aaaagtacta tcaactatag ctaagctttt attttcatga
    26041 gcaatttctg ctattgtttt tatatctact aagtctaatg atggatttaa aggactttct
    26101 atataaacta ttttagtatt tggtctcatt gcttctttta cattttgtgg atttactgca
    26161 tctacaaatg taacttcaac tccaaattta gataacaact cagacatgaa taatttagtg
    26221 tgtgaaaata ttccatctga actaactata tggtcaccac tttttaccaa tgctaatata
    26281 gtagaggtta ttgcccccat ccctgatgta ctagatatgg cactttctgc attttcaagc
    26341 atagccaact tagcttctaa ctctgtaaga gtaggattac actctcttgt atacacatat
    26401 ccccatgtca tacacaactc ttcaaaatgc tctactgtct ttgcagcaaa tgttgctgtt
    26461 tgacatattg gtgtagccaa tgcacctgtt gttgggtctg cactatgtcc ccaatgtatt
    26521 atttttgtct ctaaatcttg attaatattg ttactcattt tgatttcctc ccattttgac
    26581 ttatttatac aacgttttcc ctgccaaatt tcatcaattt agcttagtct taatacattt
    26641 tttcaagtat acttttttat ttgttataat ttctaaaaat atgacactta ttttgttttt
    26701 tacagttcta attataaact ctatagtaac tatagagtca atatgttttt gatatttttt
    26761 ttacaatgtt taaaatagtc agtacaaaaa taggtatatc ttagaataac taaagtcatc
    26821 ctaagatata cctattttta tatcacaata taaataatta catataagca ttagtttttt
    26881 tatcataaat agatttttca agatttatca tcttttcata aatatattat agttaaatat
    26941 tttaatatta cctaacatct acaataaaat atactcttaa aataaaatta tataattaaa
    27001 aatactctat aaaagctaaa aagtttttct attcgctaca tctaaaaata attagataga
    27061 ggtataaatg tatttagttt atttatacct ctagtctttt ataaattata tctctactaa
    27121 attgattttt tagcacgaat aagtgtgctc ttggagatac cagtcatctt cacaacctct
    27181 ctatatggct tagaatttaa taaccccaat gcatattgta tctgttgatt gctgtatttc
    27241 tttggcctac catctttaaa gtcctttttc tttttagcaa attcttttcc taattgagta
    27301 cgttctatta tcatatttct ctcaagttct gcaactgcaa gtaatgtagt caaaaagaat
    27361 ttacccattg aagtattttc taaaaggcct atatttaaaa tatgaacact tacttcttta
    27421 gcaaataaac cttctattag ttgtattcct tctgatgtac ttctagctag tctatccagc
    27481 ttagttgcaa caagtaagtc tccctttttt agagatgata taattttttc aaatttaggc
    27541 ctgtttgtag tagtgcctgt ataagtttcc tttattattt tagcatcatt atatttattc
    27601 aaaatttctt gctgttgctg atacaaactg ttgttgtctg tttgattttt tgtacttaca
    27661 cggcaatatc catatataat aatcatcact tcctaacata aagattttgt acagaagtta
    27721 cccagaagct tgtttttact tatctttatc cattttacta aaacgatttt tgggtgtcaa
    27781 tactgttaac ttttgacacc ataataattc ctttataatt tgtttgacta tgtcaaaaat
    27841 taccaatata atgaaattaa tgagtttata caaattggag gaaataatat gaatagtctg
    27901 ttaaaagaag caatagatga attaaaaaac atccattgcg gggaagtttt tttattaaaa
    27961 gaattgttca aaggctatga atggaacagg ttggagatga atatcagatt gaaattagga
    28021 aaactttttt tgtatgaagt ggtaactaat cccaatttaa atgtagaggt catcaataaa
    28081 aactctgctt atcagcaaat gtataaaaaa aaatagtgac ttaaatgtat ataatagtat
    28141 aatcttctta ttgattcaaa agttaaaact tttaatacaa aacaatcatt ctattttaac
    28201 atttatcaat atgtctaaat ttatataatt aacacatacg aatagtattg tttaaatcac
    28261 attatttaag atagataaat cttaacccta ctccagcata aatactaaaa tagggcttag
    28321 aattatctat atttttatcc cataaaatgc ttagctcttc ctcttgattg aacatatata
    28381 tctggggaaa tacaatagaa gcgtaaattt ataaaatttc caaagtcaga aaacttcagt
    28441 tcaaacttag gtataccaaa actttcaggt gctatttcgt caacaccaaa atagatgata
    28501 attccattat ctgtaagata aaatgcttgg tcttcaggta taactaactc aacattagaa
    28561 taatacattt ctctattttc atttatttga gatttaatgt aatcagtaat tatgttaata
    28621 taatcaactc catctctaaa aatgtcatta agtaaaagtt cttttccagt tgtcaaatct
    28681 atattgtaat tattcaatgt attgtattta ggcccatagt tattagcaaa ccccataagg
    28741 tttaatatca tactcagtac ataattttta ttaaaagtaa ctgcgtaact ggtagatacc
    28801 caatagcttt gttttggaag tgaattttga gaagctgcgt tattatattc tcgttcttct
    28861 tcctgtaagc catttttaaa attatttaca gtcattctta cagcattgtt tatgttattt
    28921 aaaataactg ggtttacata cgtggattga tttctaatat tgtatataac aggatacttt
    28981 acatcatatg aaaaatactt acttctttca aaatcttcat taaattgtac atataatata
    29041 ttatatgtac aggtatttgt agattgataa ttttccatta aaatcctccc ccttttataa
    29101 aaaaatcaga tacaatataa tatgcttaaa acagtaataa tgttaatggt aaatgtaaag
    29161 aatagaaagt atctaaaaca gcaaaaaatg actaattttg tggtataata cataggattg
    29221 agagttattt tataggagga aaaatatgtt attattagca gataaatgga aagattatga
    29281 acttatagat atgggaaatg gagaaaaatt agaacgttgg ggaagttatg tacttagaag
    29341 accagaccca caggttgtat ggccaatgga atctgaatgg gctttatgga aaaaccctca
    29401 tggacattat cacagaagca ataaaggtgg aggtcaatgg gaacacaaaa aaagatatcc
    29461 agaacaatgg actataaatt ataaaaactt aaagtttcac attaaaccaa ctggctttaa
    29521 acatactgga ttattcccag agcaagcagc taactgggat tggatgatag agaaaataaa
    29581 gaaagctaac agacctataa aagtgttgaa tctttttgca tatacaggtg gagctactgt
    29641 agcatgtgca tctgctggtg cagaagtatg tcatgttgat gctgcaaaag gtatggttaa
    29701 ttgggcaaaa gaaaatatag aattatcagg tcttaaaaat caaacagtaa gatttatagt
    29761 ggatgatgtt gtaaagtttg ttgaaagaga aataagaaga ggtaaaaaat atgatgcaat
    29821 aataatggac cctccttctt atggaagagg tcctaaagga gaagtatggc aaatagaaga
    29881 aaagttatac agttttgtag atttatgtat gggagtttta tcagataacc ctttattttt
    29941 cttaattaat tcttatacaa caggattttc tccaatagtt cttgaaaatg tattgtctac
    30001 aacaataggt aaaaagacaa atacaggaga tatatacggt ggggaattag gaataccagc
    30061 ttccagagat ggtaagatac ttccttgtgg tatatttgga agatgggaga aaaaataatg
    30121 ataaaagtca tatatgaaga taaccattta ttagttgtag aaaaaccagt taacatattg
    30181 tctcaaggag ataatactaa tgataaagat atggtaaatc ttttaaaaga ctatattaaa
    30241 aaaaagtata acaaacctgg aaatgtatat attggtttaa tacatagatt ggatagacca
    30301 gtaggtggag ttatggtgtt tgcaaaaaca tcaaaagcag catctagatt gtctgagcag
    30361 gtaagaagta agactttcaa aaaaacttat ctagctgttg ttcatggcaa gataaaatct
    30421 aactcagata tactaaaaga ttttttatat aaaaataaaa aaactaatat ggtaactgta
    30481 gtgaaaaaaa gccataaaga tgccaaagaa gctgaactta actataatgt attagatttt
    30541 aaagaaaatc taagtttagt tcaagttaat ttaaagactg gtcgttctca tcaaataaga
    30601 gttcaatttg caagcagaaa acatccatta tacggagacc aaagatatgg caaagaaata
    30661 aataaaattg gacaacaaat atctctttgg tccaataaga tagaaatttg ccatcctact
    30721 acaaaagaaa aaatggaatt tacatgtcat ccacctgaca aatatccatg gaatatattt
    30781 taaatctcaa atgaggtgat tatatgagta aaaaattttc aataggagaa atgtctaaac
    30841 ttcataaaat aagcatacag acactaaggt actatgacca aatagattta tttaaacctg
    30901 ttcatgttga ccaagaaagc aattacagat attactcaat tgaccaattt tcacaattag
    30961 atattataaa atatttaaaa tatttaggta tgcctttaaa agaaattaag tcaaagcttg
    31021 atgaaaataa tgaagaaatt ctaaaattac taacaaataa aattaatata gtggatgaaa
    31081 aaattaaaga attagagctt ataaaaaagg tactaagcac aaagaaagaa actatggaaa
    31141 aaggtatgaa agtagatgaa gttggtaaaa tagtgagaaa acatattcca cgaagaagta
    31201 tcttatctat agagtatgaa gaaggattaa gtagtgaaga aactatggaa ctttccagaa
    31261 gacaaatagc aaatatatta gaagataata tatctgtgtt ctatggtgga gtaagtggac
    31321 ttgtatctat agataaattt ttaaaagaag ataaagttat ttatgacaat agttttgtta
    31381 ctgttgaaag agacttattt gatataaaat cacaaaaaca tataacagaa atttcatctg
    31441 gtgattttat ttgtatgaca tataaagggc cttacaaaga aaattatacc gcttttaaga
    31501 aactggttga ttatgttgaa tataaaagaa tacctattga aaattatata tatgaaatac
    31561 ctataataga ccctctgtct acaaacaacg aagaggaact tctaacagaa attcaaatac
    31621 aagttaaaaa gagagaaatt gacttaagat gtctagtgta gtaatctagt ttacttacac
    31681 atcttaatat agttgtctat tatcaacata tgatttttct agatagtgat tcctaacagt
    31741 gagattttca aaaaatagga ggaaatatgg ctaatgctaa ttccctccta tttttaatta
    31801 atccatatca ttttaattaa gccatgtcat cacgaagtgc atcaataata ttttcttttt
    31861 ttatcttatt aatagtatac aacattgtaa taaatactat aaataaaaca ctaaatatac
    31921 taattccaat actaaaccat ggaatcacaa aatcaatatc atctgtaacc attactttat
    31981 gaattagcca tgaagaaatt actgctatag gaagtccaaa gaacaatgct ctcatgccat
    32041 aaaaggcaca ctcaaaattc atcatctttt ggaaatcatg ctcagacatt cctacagagc
    32101 gaagcatggc aagttctcta cgacgtaact taatattagt tgaaattgta ttaaacacat
    32161 tggcaatcga aatcagagaa atcataatta tgaaagtata agcaaacaca ttggcaataa
    32221 aaatgtaatt acggttctca tccatcattc catacatatt gtacagattg tactcattcg
    32281 taactccctc actctcaata atttgctcca tttctgcaac tgactgagta ggatttttcg
    32341 attgaaagct taaactttca attcctgtag aaacaccagg aatctcaaac ttatctttgg
    32401 ctgaataagg agccaatact ctgaagcttg ctttcttcac atctgaactt ctgattcctg
    32461 gaggagaatc aggtggcact actttgacaa atgaaatatt cacattttgt ctctcaggta
    32521 tctccaactt accattagtt tcaggagtga ggctaacatt catagaagaa ctgttgaaaa
    32581 tatcaataat attattatcc tttgttacca tagaaacagc aatcagtttt gcattttgtc
    32641 cagtatattc ttctgctggt aatccaagac ttttaataat attcagataa gttctatcat
    32701 caagaaattg catatctact ggtaattcaa gcatttcact catagaatgt gaaccctcat
    32761 agttcaaata atcgtctgta agctcactta ctttggcaac acatgaatag gtcataagtt
    32821 tctgatacaa accttcgtaa acaccatttg cagtttttag cttatcgtaa agcttcaaca
    32881 tttcgttgtc tttcatacct tgtgtagaaa aaataatatc ctggtcagta acctctgacc
    32941 tctgatttga catctgtttt aggtctgcta ctaaagcact tgtagatata aataaaacta
    33001 tgcttaaaac aagcgacaat acaatacttc gataacgttt cttgtttctt ttaaagtttt
    33061 tcatagcaag aactccctca agaccataaa ctcgctgtgt tagttttgat gtcttcacag
    33121 atttagattc aatttttacc tcatttgtct gacgaataca ctccatcact ggtgtgttgg
    33181 cagcctttct tgctggtatg taagcagaaa ttaagattgt aaccatactt accactgctg
    33241 caacgatgat tgcagggata gacaatgtta atgtcaacgg aacgtttaca tagagaatat
    33301 ttgcaaagtt tttaactatg acagaaatca caagtccaat actgactata ccgattgcaa
    33361 caccaattgg tataccaatc gcaccaatgc aaattccttc aaatagcacc gaatttcgta
    33421 actgttttgc tgtagctcca acagatgaaa gtattccaaa ttgatgtgtt cgctcgttca
    33481 atgacatgtt aaatgaatta tatattagaa aaattgagcc tagcatgatt atggcaagca
    33541 caatgccacc aaccgagtac aaaaacatat tgaacaaatt gttatcagaa acacctatga
    33601 agcgtaacac attgtcattg aaaatggaag catggtcttt agtgctactt gtgtaagagc
    33661 gaatctgatg tggatttttc aatgtgacaa aaaggctaag actgtcagtc ttgacttgtt
    33721 tatcttcttt ggttatcaag gtatatcctg gtgcagaaga ttcttcaaat acgggctttc
    33781 tacagatacc cacaactgta taagttttct tatcctgtgg cacaaaaact tcactcccag
    33841 cagtgtatgg gtcactctga ccaagctttt cattttcact cattcgtttc cctacatcca
    33901 aagaaatagt atcacctact gcaaatttga caccaccatc tgtcgcaact ttacttgaaa
    33961 caagaatctc tctactattt tcaggtaatc ttccagaaat taaagttata ggcaaagtat
    34021 caaaagcttc tttgctaaat ccagcaataa aaagatatgg tttatctgga tttctgttac
    34081 cttcaagtgt tgcatagcca atattttcaa atgttgcagt atttgcaact tcatcattga
    34141 ctctttgttc ctgtacaaaa gaagaatcca catccaaaaa ctggacatgc caatcaccat
    34201 attttataat agctccattt atcatatagt tcatcaaaga aactccaaag gtagtaactg
    34261 ctgtaatcat agcagctgac agaacaactc ctataattgt tacaagagtt cgagttcgat
    34321 tctttttcaa tccttgcaga gcaactttgt taaaaatgtt cattaacgat tcaccttcct
    34381 ctcatcacgc actacttttc catctgaaat acagataatt ctatctgcct gtaaagcaat
    34441 gttttcatca tgtgtgacaa taatgagagt ttggtgatat ttactatggc tctctttcag
    34501 taactggata atctcttgtc catttttact gtctaagcta cctgtaggct catcagcaag
    34561 cataactgct ggtgcattca tcaatgcacg accaatggca acacgttgct gttggccacc
    34621 agaaagttga tttggtaaat gtgtttttcg ttcttttaaa ccaagtaatt ctaacaagtc
    34681 atttaaccgt tcttgattga ccttacgctt atccataagt ataggcaatg tgatattttc
    34741 tactacattt aatgtgggta tcagattgtg aaactgataa atcaatccaa cttgtcgcct
    34801 acgaaaaata gcaagttttt cattgctttg gccatataca tcttgcccct ccagatatat
    34861 ctttccactt gttggtacgt ctactcctgc aatggaatgt agtagtgtag atttaccaga
    34921 accagaagaa ccaatgattg cagtaaactc tcctttttca atagtaagtg agacatggtc
    34981 aagtgcagta accttatttt cattcttacc ataaacctta cataaatctt caatctttaa
    35041 aaacgccatt atataagtct cctttcatat tttataaata tatcatagga ctttcaactt
    35101 acatttaagt gacatttaaa tgagagattc gtcactttgg aaaacgaata acaaatattg
    35161 caccaccttg tgggtgattt tgtgcagtaa ttgttcctcc ttgtctcata ataatcgtct
    35221 tacaaagtgc caatcctatt ccataaccgg ctgtgcttga atttttccca cgataaaatc
    35281 tgttaaatag acatggtaaa tcttcttttt taaagcctgc tccgttgtca tgaatagtaa
    35341 gttgagtaaa caaaaagttg tcctcacaaa tgatatcaat ttttccatta tcacccacac
    35401 tctccatgca gtttttaaat atattttgaa ttgcttctga aagccaattt aaatcaccca
    35461 aaatccttac tccttttggt atatcaatat gtaaagtgat attgtgcagt tccatgggaa
    35521 tcaaaagttg atgaagtgca gaactaatca aattattcac atcaatttgt tctttttgaa
    35581 agactacaat acctgcatct aaacgtgata attttaaaag ggaagtaatc agccagtcca
    35641 ttcgtataag taattcctct gtttctcgta tcaactcttt tctctcatct tcatcagaag
    35701 tattctctaa cagtgacaaa ataagagtta ctgatgtaag aggagtgcga agctggtggg
    35761 ctatgtcagc tagtgagtca gcaaggtgtt ctttttcttt tttcaatgcg taattttgct
    35821 ctctaatacg taacgtcatt ttagttattt cactttgcag aatagaaagt tcaccctcat
    35881 ctgattcagc aatatacaga tggttagcat tatgaagcac aagattaatt tgttctgaaa
    35941 tctgtgcaat acttttgtat cgatttttag taaacacaaa aaatgctatt ccaaaggtag
    36001 tggcagaaac aatagaaagt attccagtca taatactgat tgcaaatccc agggctacag
    36061 tgagaatagt aattaataag aataaaatag caaattttcg aaactctcta tttcgaaaca
    36121 taacttcccc ccaatctata ccctgtccca cgaacagtca aaattatttg tggacttgag
    36181 gggtcatctt ctatcttctc tcgcaagcgt ttgatgtaca cagtcaatgt gttgtcatta
    36241 acaaactcac ctgcagcatc ccacaattca tcaagtaatc ttaccctcgt aataatactt
    36301 ttaggattgc taataaacac caataacaaa cggtattcta aagctgaaag aaaaacctca
    36361 ttgccattct ttttcacaac accggttgct gtatctacat aaagcccaca aatttcaaaa
    36421 gttgacccaa aacgtccact ttttcgtagt gctgttctaa ttcgcgcaat cagttcacgt
    36481 ggacgaaaag gtttagtaat ataatcatct gcacccatat tcagtccagt aacaacactt
    36541 gattcatcac cagaagctgt caaaaagata acgggaatgt cttgcttttc tttgatttct
    36601 gtacaaatcg taaacccatt tccatcaggt aaagaaatat caataagtgc caaatcaaat
    36661 ttattcttag caatctctac aagtgcttca cttcttgtgg atacatgggt tactataaat
    36721 ccttctgaat taagcaaaag aataagattt ttggcaattg ctttatcatc ctcaactaaa
    36781 aatatccgtt tcatttactt tcatcctcct ttattttatt agttttatat attccagcag
    36841 agcaatagta cactactatt ttacttatca ttacacaatt ttcagtagtt tcttttgttc
    36901 ttatataatc catgcataat acctccattg atttattata tttcttataa cataaataca
    36961 caagataaga caaaaaacat aatgtaagtg caagtttttt aactcactaa cttataaaca
    37021 tacactccta ctaacactac tttcttgatg acacatctta caagcttata ttcatacatc
    37081 aatgaaaaat taaatttatc catttattaa aataaaaggc aatagcttct atagctaatg
    37141 ccctttatct taaaatgatt taaatactat acaattttta taaatggcta taaataaact
    37201 cgtatagaat ataaaattta tctacagtct tttgttatac aaaatttata acctcttata
    37261 gaacaaattt accactttgc aaacttatca cttttactca gctatattat aaacttttaa
    37321 tttatctttg tttaaatgtt tcttcttaac ttctactaaa gcttttagcg tatccagaga
    37381 tgtcattatt tcagtagaaa attcaatagc tgttcttctc attttaaagc catctcttgt
    37441 agagtcattt cctttagttg gtgtatttat tactatatca acttgtttat ttcttatagc
    37501 atctaatatg tttggtctag gctcttcaat tctatttact atatcagcct ctattccatt
    37561 ttctcttaaa ctcttcgctg ttccttctgt ggcaacaaaa gtatatccta attctttcat
    37621 atcttttgct atctctataa actcatcttt atcatgattg tttacagtag caagtactac
    37681 tcctctttca tcagacatat gtctacctgc tgccaagaat cctttatata aggcttcttc
    37741 taaattttct cctacaccta gaacttctcc tgtagacttc atttcaggtc ctagacttac
    37801 ttcaacttta gataatttag acattgagaa tactggtact tttactgata ctaattttgg
    37861 ctctttgtaa actcctgtac catatcctaa atcctttaat ttagctccta acatacactt
    37921 agtagccaag tcaactattg gtactccact tactttactt atatatggaa ctgttctact
    37981 agctctaggg ttaacctcaa ttatatataa ttcattttga aattctataa attgtatatt
    38041 aaccatacca agcacattaa ggtctaaagc tattttttta gtgtattcta ctatcttttc
    38101 ttttatttca tcagatatat tttgacttgg atacattgtt gtactatctc cagagtgaac
    38161 tccagctctt tctaaatgct ccattatacc aggtatcaat atatcttctt tatcacatat
    38221 agcatctact tctatttctc taccagttag atatttatct ataagtactg gatttttatg
    38281 gtctctatca aatgcatcat ccaaatactg aattagtttt tcttcgttat aagttatttc
    38341 cataccttga ccaccaagta cataagatgg tctaactaat actggataac ctaactcctt
    38401 agcaatttca acaccttcat tagttgtcca tacacctttt ccttttggtc tatttatatc
    38461 tagtctctct agtaaatcat caaatttttc tctatcttct gctgcatcta tatctctaaa
    38521 gtctgttccc agtataggta tatttttttc atgtaaaaac ttagccaatt ttatagctgt
    38581 ttgtccacca aactgtagta ttacaccttc tggtttttct ttttctatta tatttaaaac
    38641 ttcttcttct gttaatggtt caaaatacaa cttatcagat gtatcaaaat ccgtacttac
    38701 agtttcaggg ttattgttta caattatagt ttctattccc atttttctta aagattttac
    38761 acagtgaact gaacaatagt caaattctat tccttgacct attcttattg gaccagaacc
    38821 caatactaca acttttctct tatcactaac tactacctca tcatattgtt cataagttga
    38881 atagtaataa ggtgatagag cttcaaattc tcctccacat gtatcaacca ttttataaac
    38941 tggttgtata ttatataaac ttctcaattc atataatttt tctgggctta tcttcattaa
    39001 gtcagatata cctttatcag aaaatccctt tttctttaat tcatgtaaat attctttatc
    39061 taagtcttct atttttagaa cttttaactt ttcttcttgt tctactatcc atttaaattt
    39121 atttataaac cacttatcaa caccagttat ttctgatatc atttctactt tatagcctct
    39181 tcttatcatt tcagctaagt caaatagtct ctcatcatca ggtactacta ctctagcctt
    39241 taattcttct aatgttctac cttctgatgg agcatgaacc aatgaatatt ttcctgtttc
    39301 aagtgatctt atacctttaa gaatagcagc ctcaaagtta cttcctatac tcatgatttc
    39361 accagttgcc atcatcttag ttcctaattt tctattggca tgtctaaatt tatcaaatgg
    39421 ccactttggt atttttacta ccacataatc taatgttggc tcaaaacaag cataagtttt
    39481 tttagttact gcattttcta tctcatccaa agtataacct aaagctatct tagctgctac
    39541 ttttgctatt gggtatcctg tagcttttga tgctagggct gatgacctac taactcttgg
    39601 gtttatttct attatcgcat attctaaact atgtgggttt aaagctattt gaacgttaca
    39661 tccaccttga acctctattg catttattat gtctattgat gcctttctaa gtagttgata
    39721 ctcttcatca cttagagttt gacttggagc aacaactata ctgtctcccg tatgaactcc
    39781 aactgggtct acattttcca tattacatac agttatacag tttccatttc catctctcat
    39841 tacttcatat tctatttctt tccaaccttt tatactcttt tcaagaagaa cttgtccaac
    39901 aggacttagc tgtagtccat gtgaaagtat ttctcttaat tcttcctcag tatcagctat
    39961 acctccacca gttccaccta gcgtatatgc tggtcttaca actactggat atcctatctt
    40021 attggcaaac tcaagacctg cttctaaatt tgtaactata tcactaacta ttactggttg
    40081 atttatttct ttcattactt ctctaaataa atctctatct tcgccctttt ttatagattc
    40141 tatagatgtt cctataactt ttacattata tttatctaat ataccagcat catgtaattc
    40201 tacagctaag tttaatcctg tctgaccacc catacctgca agtaaactgt ctggtctttc
    40261 tttttctatt attttttcta taaattctat tgttaatggt tctatatata ttttatctgc
    40321 aatttcttta tcagtcatta tagtagctgg gttactattt actaatacaa cttcaattcc
    40381 ctcttctttt aatgcttggc aggcctgtgt tcctgaatag tcaaactctg cagcttgacc
    40441 tattattatt ggccctgacc ctaatactaa agttttcttt atactatcta atttaggcat
    40501 aactctttct ccttatctaa acaattttca cagttcttat cttcatcatc tatacaatca
    40561 actttaatta ctttatttta tcttaagttc aaatttactc atcaatacat ctatttaata
    40621 catctatgtt aattaaacca aactttttga tttaagttat tcttgttttt tagttagcta
    40681 ttctaatgtt atttatttta ctaattctaa aaatttatca aaaatatagt cattatcttt
    40741 tggtcctggg caagcttcag gatggtattg aacactgtat acatctagct tctcatgtcg
    40801 cattccttca acagtattat catttaaatt tatatgtgtc actttcattt gttcaggaac
    40861 ttctgaaaca taataaccat gattttgaga agttataaat acttttcctt cttccaaatc
    40921 tttaactgga tgatttccac ctctatgccc aaatttaagt ttagcagttt ttcctccaag
    40981 agctaaagct aaaagttgat gtcctaagca tattccaact ataggttttt taccaataag
    41041 agcttttata ttttctataa catcttctaa gtcttctggg tctccaggtc catttgacaa
    41101 gaatattaag tcagggttta tactcaatac atcttctgga gatgttgtag ctggaaatat
    41161 tgttatatca catccacgag ctataaatga tcttaatata tttcttttta ctccaaaatc
    41221 cataacagcc acttttggac catttccttt tatatgctct acttcttttc ttgtaacagt
    41281 tctaactgct tctgtatttg aaaacttatc caatttatgt ttaacatctt ctaatttact
    41341 gtcttctaat gtaattatac ctttcattgt tccattattt ctaagtatct tagtcaaagc
    41401 tcttgtatct attccttcaa gtccgataac tttattttgc tttaaatatt ggtctatatc
    41461 aatttcacat ctaaaattac ttggagaatc acttttctcc cttactatga atccctttac
    41521 ttgaactttt tcagactctg ctgatgataa atttatacca taatttccca caagaggata
    41581 agtcatagta actatttgac catagtaaga agggtctgtt aaaacttcac catatcctat
    41641 catcgaagta ttaaatacta cttctcctac actttcttct aaatatccaa atgcctttcc
    41701 tataaaaact gttccatctt ctaatattaa ccttgctttc atactacagt acctcctata
    41761 attctcttta gacaaactaa agaacattaa ctaaatataa gcttttaaat atctatatct
    41821 atttttcact tttacattct agttttatac ttatttacat ttatttttta tctttttcta
    41881 ttatactaca aatttcttaa aaaaaagaag ttatctcaaa agtaaaaact cctagaaaaa
    41941 ataacctctt tttttttatt taataattaa attcttaaac tatacaatca acttaatttc
    42001 tattcagcta tattgtaaac ttttaactca tctttattta aatgtttctt cttaacttct
    42061 actaaagctt ttagtgtatc cagagatgtc attatttcag tagaaaattc aatagctgtt
    42121 cttcttatct taaacccatc tcttgtagag tcatttcctt tagttggtgt atttattact
    42181 atatcaactt gtttatttct tatagcatct aatatgtttg gtctagattc ttctactcta
    42241 tttactatat cagcctctat tccattttct cttaaactct tcgctgttcc ttctgtagca
    42301 acaaaagtat atcctaattc tttcatatct tttgctatct ctataaactc atctttatca
    42361 tgattattta cagtagcaag tactactcct ctttcatcag acatatgtct acctgctgcc
    42421 aagaaccctt tatataaggc ttcttctaaa ttttctccta cacctagaac ttctcctgta
    42481 gacttcattt caggtcctag acttacttca actttagata atttagacat tgagaatact
    42541 ggtactttta ctgatactaa ttttggctct ttgtaaactc ctataccata tcctaaatcc
    42601 tttaatttag cccctaacat acacttagta gccaagtcaa ctattggtac tccacttact
    42661 ttacttatat acggaactgt tctactagct ctagggttaa cctcaattat atataattca
    42721 ttttgaaatt ctataaactg tatattaacc ataccaagca cattaaggtc taaagctatt
    42781 tttttagtgt attctactat cttttctttt atttcatcag atatattttg acttggatac
    42841 attgttgtac tatctccaga gtgaactcca gctctttcta aatgttccat tataccaggt
    42901 atcaatatat cttccttatc acatatagca tctacttcta tttctctacc agttagatat
    42961 ttatctataa gtactggatt tttatggtct ctatcaaatg catcatccaa atactgaatt
    43021 agtttttctt cgttataagt tatttccata ccttgaccac caagtacata agatggtcta
    43081 actaatactg gataacctaa ctcattagca acttcaatac cttcgttaag tgaccataca
    43141 ccctttcctt ttggtctatt tatatctagt ctctctagta aatcatcaaa tttttctcta
    43201 tcttctgctg catctatatc atcaaaatca gttcccaata taggtatatt tttttcatgt
    43261 aaaaatttag ccaattttat agctgtttgt ccaccaaact gtagtattac accttctggc
    43321 ttttcttttt ctattatact taaaacttct tcttctgtta atggttcaaa atacagctta
    43381 tctgatgtat caaagtctgt acttacagtt tcagggttat tgtttatgat tatagtttct
    43441 atgtccattt ttcttaaaga ttttacacag tgaactgaac aatagtcaaa ttctatacct
    43501 tgacctattc ttattggacc agaacctaat actatcacct ttttcttatc actaacaact
    43561 acctcatcat attgctcata agttgaataa tagtaaggag atatagcatc tatctcacct
    43621 gcacaagtat caaccatttt atatgctggt ttgatattgt atagacttct caattcatat
    43681 aatttttctg gacttatctt cattaagtca gatatacctt tatcagagaa tccctttttc
    43741 tttaattcaa gaagatagtc tttagttaaa tcttcaattt tcataacttt taatttttct
    43801 tcttgctcta ctatccattt aaatttattt ataaaccatt tatctatacc tgtaatttgt
    43861 tctatcattt ctactttata gcctcttctt atcatttcag ctaaatcaaa tagtctctca
    43921 tcatcaggta ctacaactct tttctttaat tcctcaatgc ttctctcctc agatggtgtg
    43981 tgaactaatg aatattttcc tgtttcaagt gatcttatcc ctttaagaat agcagcctca
    44041 aaattacttc ctatactcat aatttcacca gttgccatca tcttagttcc taattttcta
    44101 tttgcttttt taaatttatc aaaaggccat tttggtattt ttgccaccac ataatctata
    44161 gttggttcaa aacaagcata agttttttta gttactgcat tttctatctc atctaaagta
    44221 taacctaaag ctatttttga tgaaacttta gctattggat atcctgtagc ttttgatgct
    44281 aaagctgatg atctactaac tcttggattt atctctataa ctgcatattc aaaactatgt
    44341 ggattaagtg caaattgaac gttacaacca ccttgaactt ctactgcatt taatatatct
    44401 aaagatgctt ttttaagcat tgcacattct ttgttactta aagtttgagt tggagcaaca
    44461 actatactgt ctcctgtatg aactccgact gggtctatat tttccatgtt acatacaact
    44521 atacaatttc cctttgaatc tcttattacc tcatattcta tttctttcca accttttata
    44581 ctcttttcaa ttaatacttg tccaacagga cttaattgta gaccatgtga aagtatttct
    44641 cttaattctt cctcattatc agctataccc ccaccagttc cacctagtgt gtatgctggt
    44701 cttacaacta ctggataacc aattgtaagt gcataatcaa gacctgcttg taaatctgta
    44761 actatatcac taactattac aggctgattt atttctttca ttacttctct aaatatatct
    44821 ctatcttcac cttttttgat agattctact gaagtaccta ttatctttac tccatattta
    44881 tctagtatgc ctttttcata aagttcaact gctagattta gccctgtttg tccacccata
    44941 cctgcaagta aactgtctgg tctttctttt tctattattt tttctataaa ttctattgtt
    45001 aatggttcta tatatatttt atctgccact tctttatcag tcattatagt ggctggatta
    45061 ctatttatta atacaacttc aattccttcc tctttcaaag actggcaagc ttgtgttcct
    45121 gaatagtcaa actctgcagc ttgacctatt attattggtc ctgaccctaa tactaaagtt
    45181 ttctttatac tatctaattt aggcataaat ttttctcctt atctaaatgg tttttactat
    45241 ctgtaaataa tactaaatct atattaatgc taagaattta ttaaatatac aatctaaatc
    45301 ttcttttgtt gtgtattctg gaatgtactg aacactatat attggtaatt ccttatgtct
    45361 cattccttct atagtattgt catttaaatt tatgtgagtt acttctgtat tttctggcat
    45421 ttctgaaaca ttatatccat gattttgaga agttataaat acctttccag tttctaaaca
    45481 tttaactggg tgattacctc ctctatgtcc aaatacaagc tttgaagtct ttcctcctaa
    45541 tgccaatgct aaaatttgat gtcctaaacc tattcctgtc aatggctttt tgcctattaa
    45601 tttttttata ttttcaacta tatcatttat atcctctgga tttcctgggc cattagataa
    45661 taagattaag tcaggattta tatttaaaat ttcttctact gtagtcattg ctggtactat
    45721 agttatatca cagttacaag ccttaagaga ctctagtaca ctatttttaa caccaaagtc
    45781 tataactaca actttagctc cagtaccttt tatattttca atttcttttc ttgtaacatt
    45841 ttttactgaa tctttgttac aatgctcttc taatttagac tgaacatctt gtaatgatac
    45901 attttctaaa gttataattc ctctcttagt tccattgttt ctaataattt tagttaatgc
    45961 tcttgtatct attccttcaa gtcctattac cttattttgc tttagatatg tgtctatatc
    46021 catttcacat ctaaaattat ttgggttatc acttttttct cttacaataa gtccttttac
    46081 ttgcacctta tttgattcaa catcttctaa atttattcca tagtttccta taagtggata
    46141 agtcatagta actatttgtc cataataagt tgcatctgtc aatagttctc catacccagc
    46201 cattgaagta ttaaatacaa cttctccaat agcttccttt agatatccaa atgcttttcc
    46261 ttcaaatact gttccatctt ctaaaattaa tttccccttc atataagcta cctcccaaat
    46321 attttatcta agcattttct atttctctta caatattaat agctgattct cttggatttt
    46381 ctgcttttgt tataggtctt ccaacaacta aataatgagc accttttttt attgcatctg
    46441 atggagttac aactcttttt tggtctccaa cctctgctga tttaggtctt attcctgggc
    46501 aaactgttat aaaatcagtt ccacatgctt ctactatttt atctacttct tgcgctgaac
    46561 aaacaacccc atctattcct gattcttttg ccagtttagc tctctttata gctaactctt
    46621 ctgttgtcat attacatcct atgtcctgta aatcttggtt gcttaatgaa gttaaaactg
    46681 ttactccaac tactaatggc ttttctatat ttaatctttc tgcttcttcc ctagcaatta
    46741 gtgcacattg tctcattcct tccatatctg atacatgtat tgtcattaac caaacattat
    46801 ctctcactgc agctttcact gcacttttca tagtatttgg tatatcatgg aatttaaggt
    46861 ctaaaaatat ttttttacct ttttctttaa gataatctat tgttttacct tttgtggcaa
    46921 cgtattgctc tagccctacc ttaaatatgt ctacactatc ttctaatctg tctattagtt
    46981 ctttagcttt atcaaattcg tcagtatcaa ttgcaactat caatttttct ttaccattta
    47041 tcatttttac atctcctttt taataaagcc taaataatca taaaaaaatc ctcacaccaa
    47101 aagaggcata aggattttat ctatacataa ctctattcat tagctatgag atatctttcc
    47161 cagtctctct ggactagttt aaaagacttt attatattta aatttttatt ttatactcat
    47221 caaaaacctc tagaagattt actagaggtt atatactaac attatctata accctttagt
    47281 ctctctggac tactttaagg aatttagctg taatattttt ctttatttat ataactttat
    47341 gtatctatag gataatttaa agttttatct tttttataat taaaccacta attgcttttt
    47401 atgtcaataa gtctttgaat tattcttcat ctttatcaaa caattttttc tcaaaatatg
    47461 attctgcatt atatattgca gcaactggat tatgcccagt tactctatct ttgacaacta
    47521 aagttgttat aggagcttcc atatatttca tggctaaagt atcgtgacca acacataatc
    47581 caagaattac attaaggtca accttagctt catttaaaaa taaagcttgt cctattggat
    47641 tgcacattac ctcatctgaa cactttgata ttgtttgaga tgcttctagt ccaattgtac
    47701 tcttaggcat tgccccattt ttacatataa cagaaaatac ttcaaaccca tgatgttcaa
    47761 atattttttg cactgtttgc atttctcttt ttagtccaat acaaaatgct atgccaatct
    47821 ttttataacc acacttttta gcaaactcta taatctcttt tagtctagta tattgacaat
    47881 acccccctgc ttcaactaat ccagcattat aagctatttt tctattctct tcttcttcat
    47941 aaagttgctt tgctttttgt tgtagttcaa tttctctagt tggacatatc ttcattaatt
    48001 tatccatatt tccattagaa cagcctctaa gattacattt tccacactta tacatatcca
    48061 atatcccctt ccaaaatttt attcatcttg cgattcttct ttttctagag ttacattttc
    48121 ttcttgggtt tcttcttctg gtttcacatg ctcgactaca gttataaaat atgattttcc
    48181 tattttattc cttataggaa gtttttcata tgaaacatct attttatttt ttatacaagc
    48241 tatatcaaaa tgtcttaagg ctacacctaa gctaacctta ttcatatctt cacagtgttt
    48301 cttaggtctc ttttcaaata aatgaagacc tcgttcatcc ttatggaaat accacggttg
    48361 agaatttttt acagatggag ctcttcttgt caattctaag atatctttcc actttctatt
    48421 cctatttatc ttatttatca tatcttttac tggctttcta tcaggtctag catatctcgt
    48481 cctaaacaac tcttcatttt ttgctggata tccaaatgct attattatat aaggattttc
    48541 tattttgttt tcatcatctt cttctgtttt tattccagct agataatcta aatcatcttc
    48601 ctcatcttct tcaaaattct tattcccaat atctacaaat tccaagatat cttctctctt
    48661 taagtttcct tccaaccagc aagtagctat tcccaatgtt gtaaggcgca aaactacacc
    48721 ttctgtagca aatcctatat tctgtaaata gtcctcacct ttgtctgatg taactactat
    48781 ataatgaggt gcctttactt tacattcctt acccatcaaa aaatgtatta tatgacctct
    48841 atcaacaaca tgtgctttaa tatttaaatc ttcatttaag tattctaaat ttttacaact
    48901 tctttttatt tcttccatca atggcttttt tatcttctta ctggaaaaat ttcttacaga
    48961 ttttctataa aaaatggctt cataaagctc catttatgcc ctcctaactg aaatcttctg
    49021 acgtataaac tctataaatt tatataaata ctttatattt tttatagttt tatttccttt
    49081 ttcctcctgc cctattttga aattcataag aatatctact ctagtttaat atagcattca
    49141 aaaggcttaa attctacctt atcaatgtct atacattagc ataatttttt tatttgattt
    49201 tctatgctaa tttaaataac aacactagct attacctttt taattaagct ttgccagaa
//
LOCUS       DAEMIK010000008        48815 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEMIK010000008 DAEMIK010000000
VERSION     DAEMIK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.008"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            326..463
                     /gene="rpmH"
                     /locus_tag="KPK87_000394"
     CDS             326..463
                     /gene="rpmH"
                     /locus_tag="KPK87_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG2536593.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            541..885
                     /gene="rnpA"
                     /locus_tag="KPK87_000395"
     CDS             541..885
                     /gene="rnpA"
                     /locus_tag="KPK87_000395"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG2536594.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            917..1168
                     /gene="yidD"
                     /locus_tag="KPK87_000396"
     CDS             917..1168
                     /gene="yidD"
                     /locus_tag="KPK87_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG2536595.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            1200..1907
                     /locus_tag="KPK87_000397"
     CDS             1200..1907
                     /locus_tag="KPK87_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG2536596.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1876..2505
                     /locus_tag="KPK87_000398"
     CDS             1876..2505
                     /locus_tag="KPK87_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG2536597.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2617..3996
                     /gene="mnmE"
                     /locus_tag="KPK87_000399"
     CDS             2617..3996
                     /gene="mnmE"
                     /locus_tag="KPK87_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG2536598.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            4094..5986
                     /gene="mnmG"
                     /locus_tag="KPK87_000400"
     CDS             4094..5986
                     /gene="mnmG"
                     /locus_tag="KPK87_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG2536599.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            5979..6698
                     /gene="rsmG"
                     /locus_tag="KPK87_000401"
     CDS             5979..6698
                     /gene="rsmG"
                     /locus_tag="KPK87_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG2536600.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            6861..7646
                     /gene="noc"
                     /locus_tag="KPK87_000402"
     CDS             6861..7646
                     /gene="noc"
                     /locus_tag="KPK87_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG2536601.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7781..8554
                     /locus_tag="KPK87_000403"
     CDS             7781..8554
                     /locus_tag="KPK87_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG2536602.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8556..9419
                     /locus_tag="KPK87_000404"
     CDS             8556..9419
                     /locus_tag="KPK87_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG2536603.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9452..10594
                     /locus_tag="KPK87_000405"
     CDS             9452..10594
                     /locus_tag="KPK87_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2536604.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVINPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10727..11302)
                     /locus_tag="KPK87_000406"
     CDS             complement(10727..11302)
                     /locus_tag="KPK87_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG2536605.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11562..12854
                     /locus_tag="KPK87_000407"
     CDS             11562..12854
                     /locus_tag="KPK87_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2536606.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            12959..13561
                     /gene="yedF"
                     /locus_tag="KPK87_000408"
     CDS             12959..13561
                     /gene="yedF"
                     /locus_tag="KPK87_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG2536607.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELNNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13634..13897
                     /locus_tag="KPK87_000409"
     CDS             13634..13897
                     /locus_tag="KPK87_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG2536608.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            13912..14097
                     /locus_tag="KPK87_000410"
     CDS             13912..14097
                     /locus_tag="KPK87_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG2536609.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14200..15396
                     /locus_tag="KPK87_000411"
     CDS             14200..15396
                     /locus_tag="KPK87_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2536610.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15610..15888
                     /locus_tag="KPK87_000412"
     CDS             15610..15888
                     /locus_tag="KPK87_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG2536611.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            15925..16359
                     /gene="ssb"
                     /locus_tag="KPK87_000413"
     CDS             15925..16359
                     /gene="ssb"
                     /locus_tag="KPK87_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2536612.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16378..16605
                     /locus_tag="KPK87_000414"
     CDS             16378..16605
                     /locus_tag="KPK87_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG2536613.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            16748..17068
                     /locus_tag="KPK87_000415"
     CDS             16748..17068
                     /locus_tag="KPK87_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG2536614.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            17076..18032
                     /locus_tag="KPK87_000416"
     CDS             17076..18032
                     /locus_tag="KPK87_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG2536615.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            18043..20037
                     /locus_tag="KPK87_000417"
     CDS             18043..20037
                     /locus_tag="KPK87_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG2536616.1"
                     /translation="MSNKQAFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            20038..20487
                     /locus_tag="KPK87_000418"
     CDS             20038..20487
                     /locus_tag="KPK87_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG2536617.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            20506..21834
                     /gene="dnaB"
                     /locus_tag="KPK87_000419"
     CDS             20506..21834
                     /gene="dnaB"
                     /locus_tag="KPK87_000419"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG2536618.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            22039..22347
                     /locus_tag="KPK87_000420"
     CDS             22039..22347
                     /locus_tag="KPK87_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536619.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            22429..23718
                     /locus_tag="KPK87_000421"
     CDS             22429..23718
                     /locus_tag="KPK87_000421"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG2536620.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            24044..24119
                     /locus_tag="KPK87_000422"
     tRNA            24044..24119
                     /locus_tag="KPK87_000422"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24077..24079,aa:Lys,seq:ttt)
     gene            24126..24200
                     /locus_tag="KPK87_000423"
     tRNA            24126..24200
                     /locus_tag="KPK87_000423"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24159..24161,aa:Glu,seq:ttc)
     gene            24205..24280
                     /locus_tag="KPK87_000424"
     tRNA            24205..24280
                     /locus_tag="KPK87_000424"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24238..24240,aa:Val,seq:tac)
     gene            24314..24388
                     /locus_tag="KPK87_000425"
     tRNA            24314..24388
                     /locus_tag="KPK87_000425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24346..24348,aa:Thr,seq:tgt)
     gene            complement(24771..25763)
                     /locus_tag="KPK87_000426"
     CDS             complement(24771..25763)
                     /locus_tag="KPK87_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2536621.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(25772..26830)
                     /locus_tag="KPK87_000427"
     CDS             complement(25772..26830)
                     /locus_tag="KPK87_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG2536622.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            26955..28418
                     /locus_tag="KPK87_000428"
     CDS             26955..28418
                     /locus_tag="KPK87_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG2536623.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNLDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFNVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            28520..29317
                     /locus_tag="KPK87_000429"
     CDS             28520..29317
                     /locus_tag="KPK87_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2536624.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDISNVYEMK"
     gene            29330..31432
                     /locus_tag="KPK87_000430"
     CDS             29330..31432
                     /locus_tag="KPK87_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG2536625.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSILFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILSITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            31500..31979
                     /gene="rlmH"
                     /locus_tag="KPK87_000431"
     CDS             31500..31979
                     /gene="rlmH"
                     /locus_tag="KPK87_000431"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG2536626.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKYKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            32128..32439
                     /locus_tag="KPK87_000432"
     CDS             32128..32439
                     /locus_tag="KPK87_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2536627.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            32487..32804
                     /locus_tag="KPK87_000433"
     CDS             32487..32804
                     /locus_tag="KPK87_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2536628.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            33017..33718
                     /locus_tag="KPK87_000434"
     CDS             33017..33718
                     /locus_tag="KPK87_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2536629.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            33942..35243
                     /locus_tag="KPK87_000435"
     CDS             33942..35243
                     /locus_tag="KPK87_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2536630.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            35380..36474
                     /locus_tag="KPK87_000436"
     CDS             35380..36474
                     /locus_tag="KPK87_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG2536631.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKTIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            36476..37609
                     /locus_tag="KPK87_000437"
     CDS             36476..37609
                     /locus_tag="KPK87_000437"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG2536632.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYEVKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            37627..38736
                     /locus_tag="KPK87_000438"
     CDS             37627..38736
                     /locus_tag="KPK87_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG2536633.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            38801..39541
                     /locus_tag="KPK87_000439"
     CDS             38801..39541
                     /locus_tag="KPK87_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG2536634.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNYSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            39546..40538
                     /locus_tag="KPK87_000440"
     CDS             39546..40538
                     /locus_tag="KPK87_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG2536635.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(40726..41607)
                     /locus_tag="KPK87_000441"
     CDS             complement(40726..41607)
                     /locus_tag="KPK87_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2536636.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            41880..43268
                     /locus_tag="KPK87_000442"
     CDS             41880..43268
                     /locus_tag="KPK87_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536637.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            43296..44249
                     /locus_tag="KPK87_000443"
     CDS             43296..44249
                     /locus_tag="KPK87_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2536638.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            44309..44533
                     /locus_tag="KPK87_000444"
     CDS             44309..44533
                     /locus_tag="KPK87_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536639.1"
                     /translation="MNEKSSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIVIKLLEIRQAREATNYNIGNG"
     gene            44526..45038
                     /locus_tag="KPK87_000445"
     CDS             44526..45038
                     /locus_tag="KPK87_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG2536640.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     IFSKDRKKGN"
     gene            45070..45924
                     /locus_tag="KPK87_000446"
     CDS             45070..45924
                     /locus_tag="KPK87_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG2536641.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            46456..47250
                     /locus_tag="KPK87_000447"
     CDS             46456..47250
                     /locus_tag="KPK87_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2536642.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            47354..48160
                     /locus_tag="KPK87_000448"
     CDS             47354..48160
                     /locus_tag="KPK87_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2536643.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            48222..48743
                     /locus_tag="KPK87_000449"
     CDS             48222..48743
                     /locus_tag="KPK87_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2536644.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
BASE COUNT        19362 a         5237 c         8798 g        15418 t
ORIGIN      
        1 atatatgtca aacttcaatg atttgtaact aatttatcca caatattaac taattgttga
       61 taaaaatata aacatgttaa aattacttta tttgtgtata taattgaaaa aaataattta
      121 gaataaaatt aaatagattt ttactagtat tttcaataaa aaaaatacat aatatatata
      181 tggtagattt tgtgatataa gcgtcccaaa aactcgttga caacagtagt aaaagtattt
      241 ataattatat gtgttatgtt tactttgagg gtaaagttat tatttttata aacggaaaat
      301 aaaatctgaa aggcggtgca aaataatgag taaaagaact tatcaaccaa aaaagagaca
      361 aagaagtaaa gaacatggtt tcagaaaaag aatgaagacg tctaacggaa gaaacgtgtt
      421 aaagagaaga agagctaaag gaagaaatag attaactcat taattacaaa agggccgctg
      481 caaaggtggc ctttttgtgt aaataattac aattcatagc tgtgtttatg gaggttaact
      541 atggacttta ataggactaa agggttgaaa aaagactctg attttagaaa agtatataaa
      601 cacggcaaat cttttgcaaa caaatattta gtaatatata tacttaaaaa taaatcagat
      661 tatagtagag taggtatttc tgtttcaaaa aaagtaggaa aagctattac taggaataga
      721 gtaagaagat taataaaaga agcctacaga ttaaatattg atgaaaaaat taagcctgga
      781 tatgatatag tatttatagc aagggtatct agtaaggatg ctacttttaa agacatagac
      841 aagtctataa aaaatttagt taaaagaacg gatatttcta tttaattgta tgctataaat
      901 atggaggtta atgactttga ataaaataat ttatatatta aaggaaataa gtaagtattt
      961 atcgaattta tgtatttatt tagtgagatt ttatcaaaag tatatatctc ccttaaaggg
     1021 acctacctgt agattttatc caacttgctc acaatattca atagaggcgt ttaagaaata
     1081 tggatttgta aaaggtatgt atttaacgat aagaagactt ttaaaatgtc atccttttca
     1141 tccaggaggt tacgaccctc taaaataaaa gagagtaact attaggaggt ataaaaaaat
     1201 tgaatataat aggaaatttc ttaggattga ttcttaaggt aatatttgaa tttgttaatc
     1261 attatggtat ttctataata ctttttacga tacttgtaaa gattatttta ttaccattaa
     1321 caattaagca gactaagtca acaaaggcaa tgcaagatat acaacctcgt ataaaagaga
     1381 tacaagaaaa atataaaaat aagccagaaa aacaaaatga agaaattgtt aagttatatg
     1441 gagaagctaa aattaatcca ctatctggtt gtttaccttt attaatacag tttcctatat
     1501 taataggatt atttagcgtt ttgagagaac cagttgctca tggcgttttt gcaaacaaag
     1561 ctgcattttt agcagctgac aatgggtttt tatggataaa aagcttaaca tcaccagatt
     1621 atgtattagc agtattttca ggagcaagtg catatgtcat gcaaaaagta atgacaccaa
     1681 aagatcagtt acaaggctct atgaaagtaa tgacatatgt aatggcaggt atgtctttct
     1741 attggggatt tatattccca gcaggactta cattgtactg gacagtaagt aacttattct
     1801 caatagctca atattactta ataatgaatc ctttaaaagc taaactggct gctaactcta
     1861 aggaggaaaa agttaatgag aaaaagccta aaattaataa aaagtaagag taaagaggaa
     1921 gctataagta aagctatagc agagttgaat ttaaaagcag aggatataga agtagaaata
     1981 cttgaaaatc caagtaaagg gtttttaggt tttataggtg ctaaagatgg tacttatgaa
     2041 atttttgtta tcgagaggga agtagatgta gctaaaaatt ttatagaggt aatgttgaaa
     2101 aatgcaaatg ttgatgccaa agtcaatgta tctcaaaaag ataatttaat catggttgat
     2161 attgaaggca aagaagctgc aagtttaatt ggtagaagag gggaaacttt agattctatt
     2221 caatttttga ctggattagc tcttaataaa ataaataaag attctcatac aagagtactt
     2281 gtagatatag aaaattatag atcaaaacga gaagaatctt taataagata tgctaataaa
     2341 gttgctagag aagtagcgaa aacaagaaaa actaaaaaat tagattatat gaatccatat
     2401 gagagaagaa taatacattc agctcttcaa aatgacaaat atgtaattac atatagtgaa
     2461 ggaaccgatc catatagaag attagtaatt gagtgtaaaa gataaagtca acctcctaaa
     2521 cattaggggg ttttattttt taatattatt aaatcattaa tgattggtta aatgtaaatc
     2581 tatatgtaaa aaattatgaa ttaagaggtg ataaatttgt ttatagatga tacaattgca
     2641 gcaatagcta cagcacctgg agaaggtggt ataggtatac ttagaataag tggagaaaaa
     2701 gctttgaagg ttgcagaaga aatctttaaa tctatgtctg gaaagagtat tgaagaatac
     2761 aataagagaa cattaatata tggaaatata gtggataatg aaaatataat tgatgaagtt
     2821 ttattggcat atatgaaagg accaaattcc tatacaggag aagatgttat agaaattaat
     2881 tgccatggtg gatttatttc ggtaaagaag atattagaat taatactatc aaaagatgtt
     2941 agattagcag aggctggtga gtttactaaa agagcattct taaatggaag aatagattta
     3001 tctcaagcag aggctgtaat tgatgttata aaagccaaaa ctgatatagc tcatgaagtt
     3061 gctcaaaatc aattagaggg ttctttatca aagaagataa gagaattgag agataaagtc
     3121 actgagatat tagctcatgt agaagttgct atagattatc ctgaggaaga tattgagcat
     3181 ataacatatc aaactcttaa agaaaaaact gatgaactta aaaaagacat taaaaaatta
     3241 tatgatacag ctgaaagtgg aaaaatactt agagaaggct taaagacggt aattgttggg
     3301 aaaccaaatg taggtaaatc ttcactactt aattctattt taggagagaa tagagcaata
     3361 gttactgata taccaggaac tactagagat gttatagaag agtttgtaaa cataaaaggt
     3421 atacctttaa aaattgttga cacagcaggt ataagagata ctgatgatat tgttgaaaaa
     3481 ataggtgttg aaaagtctaa agaatcattt actagtgcag atttgatagt aatggtttta
     3541 gatgcatcta gaaagttgtc agaagaagat atagagatat tagaaaaact taaggataaa
     3601 caaacaatag tgctgttaaa taagaatgat ttgaaacaag agattgaaga agaaaaaata
     3661 cttaaatatg tagaaaataa tagtataata aaaatatcag ctttacaaca agaaggaata
     3721 gaagaattgc aagataaaat agagtctatg gtatataaag gtagtatcaa aaataattct
     3781 agtttagtag taacaaattc aagacataaa gatgcactta gtaaagcata taaatctgca
     3841 acagatgctt taattgcttt ggaacaaagt atgccatttg actttgtaga agtcgattta
     3901 aaaaatatat gggactatct tggatatatt aatggtgata cagtaacaga ggatttacta
     3961 gataatatat tccataattt ctgtattggt aagtaatgta caaaagtaaa cttgttttta
     4021 ttatagagat ttttaaaaaa tttgaagtgt ttctcatgaa acattaattt tgaaaagaaa
     4081 ggaattataa atgataaagt ttgaagcagg aaaatatgat gttatagtag tcggagcagg
     4141 acatgctgga tgtgaagctg ctttggctac tgcaagaatg ggatataaaa ctttgataat
     4201 aacaatgtct ttggattcca tagcattaat gccttgtaat ccatcaattg gagggactgg
     4261 taaaggtcaa ttagttaaag aaatagatgc cttaggcggt cagatgggat taaatataga
     4321 caagacatat atacaaagta gaatgttaaa tactgccaaa ggtccagcgg tacactcttt
     4381 aagagcacaa gcagataaat ttaaatatca tgaagaaatg aaaaagacat tagaagatga
     4441 accaaatctt gatatagcta tggatgaagt tgtagaaata cttcatgaag gaaatgttgt
     4501 aataggagtt ggaactaaac taggatgttc atttaaatct aaagctgtaa tattggccac
     4561 tggagtttat ctgaactcaa agatatatat gggtgaagtt gcattttatg aaggtcctaa
     4621 tgcattgggg tatgcaaaat atcttacaga tagcttagtt gagcttggtc ttagaatgag
     4681 aagatttaag acgggaactc cagcaagagt tcatagagat agtatagact tctctgtaat
     4741 gtctttacaa gagggtgatg aaaaagtaac tccattctca tttatgaatg aaaatataga
     4801 aaaaaaacaa gaaccttgtt atttaacaag aactacagaa gaaactcaaa aagttatact
     4861 ggataattta aaaaggtctg ctatgtatag tggagtaata gaaagtactg gtccaagata
     4921 ctgtccttct atagaagata aagtagtacg atttagtgat aaaacatcac atcaattatt
     4981 tatagaacca gaagggttaa atactaaaga aatgtatata caaggaattt caactagttt
     5041 accttttgaa gttcaattag atatgtacaa aactataaag ggacttgaaa attgtaagat
     5101 aatgagacct gcatatgcta ttgaatatga ttgtgttgac ccaactcaac taaaaataag
     5161 tttggaaata aagggtgttg agaacttatt tagtgcaggg caatttaatg gaacttctgg
     5221 atacgaggaa gcagcagctc aagggcttat ggctggtata aatgcagtta gaaagataga
     5281 aggaaaagag ccatttgtat tagaccgttc agaagcttat ataggtgtct tattagatga
     5341 ccttgttact aaaggaacta acgagcctta cagaatgatg acatcaagag cagaatatcg
     5401 actatattta agacaagata atgcagatat gagattaact caaaaaggtt atgatatagg
     5461 attagttaaa aaagatagat atgaaaggtt cctaaataaa aaggctgctg tagagaaaga
     5521 atttgaaaga ctaaaaaatg aaagagttac accaaaagaa gtaaatagtt tgttagaaga
     5581 aaaaggagca acaccaataa aggttggaat atctctatat gaatttttaa agagaccaga
     5641 ggttacttac gaattgcttg aagaattagg gaaaggcgct ggagaagatg tttcaagaga
     5701 agttaaagaa cagtgtgtaa taataactaa atatgaaggt tatatagaga aacaattgaa
     5761 acaaatagac caatttaaaa aacttgagaa taaaaagctg gatgagaaaa taaattattc
     5821 atctatagaa ggtcttagat tagaagctag acagaaatta gatgatataa aacctatttc
     5881 aatagggcaa gcatctcgta tatctggtgt ttctccagct gatatatcag ttttacttat
     5941 atacctagaa caaatcagaa gaactagagg tggcaaaggt gaataatata gaacttctga
     6001 aaaacgggat tgaaggtttt aatatagata cgaatgacag tatgttagaa aaatttaaaa
     6061 tctatagaga gatattagtt gaatggaata aaaagatgaa tcttactgga atagaagatg
     6121 aaaaagaagt gtatataaaa cattttttag actctatttc agctgtaaaa aatggatata
     6181 taaaaaatgg gatgtctata atagatgttg gtacaggtgc tggttttcct ggaataccat
     6241 tgaaaatatg tttagatagc ttagaactta ctttgttaga ttcgttaaat aaaagaataa
     6301 actttttaga ggaagttagt agagtactag agttagataa tattacattt atacatggaa
     6361 gagctgaaga ttttggtaaa aatgaagact atagggaaaa atatgatgtt gctacggcaa
     6421 gagctgttgc tggattaccg attcttatgg aattttgtgt accatttgta aaagttggag
     6481 ggtattttat ttgcttaaaa ggtccgaatg ctaatttaga gctagaagaa tcacaaaagg
     6541 ctattgatgt tttaggattg gagtatattg aaaaaataga tgtagagtta ccagaggtag
     6601 atttaaatca taatatatta gtttttaaaa aagtgatgga aacaccagtt aagtacccta
     6661 gaaaagctgg taaaccagcc aaaaatccta ttaagtaatg aaaaatttat tattggtaag
     6721 cttaattatt tctcaggaaa taattaagct tactgtaaaa aaacaaaaat aaaggaatta
     6781 ttaaagtaga tgcagaaata aatttgtatg tacatataga gaacaaaaat aatttatatc
     6841 taaaataaga gaggtatgtt atggaagata aaagagttat ggagatacct atagaagatg
     6901 tagttccaaa tccatatcaa ccaagaaaaa tattttcaca agtttcattg gaggaattga
     6961 gcaattctat aaaggtatat ggtataattc aacctataac agttagagca aaagatggca
     7021 aatatgaact aattgcagga gaaagaagat taagagcagc taaattagca gagcttaaaa
     7081 ccataccagc aattataaat aatatgaacg atgaatcttc tgctgttcta gcgttattag
     7141 aaaatttgca acgagaagac ttaaatttca ttgaagaagc aattggttat gaaaacttaa
     7201 taaaagaaca tgcttttact caacagcaat tagcagaaaa attgggcaaa aatcaatcta
     7261 ctgtagcaaa taagcttaga atattaaagc ttccaaacga tataaaaatg aagctaatag
     7321 agaataattt aacagaaaga catgctagag catttcttaa actaccaagt gaagatttaa
     7381 tgcaaagtgt gttagacaaa gtcataaaaa atgaattgac tgttaaaaaa acagaaaaat
     7441 tgatacaaga tgtattagaa gaaactaaag ttcaagaaga gcctgataaa aaacagaata
     7501 taaagggtgc tatgagtata agaatttaca taaatactat aaagcaggcc tttgatgcta
     7561 tatcaaatac aggaattgat gctaagtata atgaaataga taagggagat tatatggaag
     7621 ttgttgtaaa aatccctaaa aaatagagat tactgcttac cagtaatctt tttttgtaat
     7681 ataagccttg taatataaat tgatttatag acattattta tacttaaaaa acacctattc
     7741 aacatttaaa attaatttaa ttcttaaagg ggggaacatc atgggcaaag ttatagctgt
     7801 attcaatcaa aagggtggag tcgggaaaac tacaacaaat gtaaatttaa gtgctagctt
     7861 aggaactttg ggaaagaaaa tattagtatt agatttagac ccacaaggaa atacaactag
     7921 tggatatggt attaataaaa acgaagtaga aaatacaatc tatgaaatta tgttagatgg
     7981 gttacatatt aaagaagcaa taataagtac tgaatttgag aatgtagatg tagttccatc
     8041 agctactgaa ctatcaggtg cagaaataga gcttacatct aaaactaata gagagtatat
     8101 tttaaaaaat tccataaaag cggtaataga tgaatatgat tatatatttt tagattgtcc
     8161 accctcactg gggatgctta caataaattg tttaactgct gtagatagtg ttttaattcc
     8221 aatacaatgt gaatattatg ctttagaagg tgttagtcaa ttaatggaga ctataaaatt
     8281 agtaaaatca agattaaatg ctgatataga aattcaaggt gttgttttaa gtatgtttga
     8341 tggaagagct aatttatcaa tacaagtagt tgaagaagtt aagaaatact ttaaaggtag
     8401 tgtatataca actttaattc caagaaatgt aagacttgca gaagctccaa gtcatggaaa
     8461 acctgtaatt tattatgata aaagatgtag agggtctgta gcttatttgg agctagcaga
     8521 agaatttata gatttagaag aggaggaatg gtagtatgga gaataagtct aaaagaagta
     8581 atagattagg tagaggtctt agtgcattga taccagaaat taaaggagaa acttcagaaa
     8641 aagaaatagt caacatagat atagacaaaa tatatccaaa cgaagttcaa cctagaaagc
     8701 aatttgatga agaaaagata aaagtattgt cggattctat aaaaaattat ggtgttttac
     8761 aaccaatagt ggttaaaatg gatgaaaata ataaatatat gataatagct ggagaaagaa
     8821 gatttagagc atcaaaactt gcaaataaaa atcaaatacc tgctataatt aaagatatag
     8881 atatgaaaga tataatggag atagccttaa ttgaaaactt acagagggaa gatttaaatt
     8941 caatagaaga ggctctagca tataagagtt taatagagca ttacaatgta actcaggaag
     9001 aaatatcaga agctgttggg aagagtagac ctcatataac taatacacta agacttttaa
     9061 atcttggaca agatgttata gaaatgatag acagtggtag aattacagca ggccatggta
     9121 aggctttatt gagaattgca gataaagatt tgcaacttca aatagcgaaa aaaatagaag
     9181 aagaagaact ttctgtaaga gaagtcgaaa atatagctaa aaaaatatct gaaaataaac
     9241 aagaagaacc taaaaaatct aaacctaagg atgtatttat tttagatgtt gaagataaac
     9301 ttagaaatat atttggaaca aaggtgaata tttctaaagg aaagaaaaaa ggtaaaatag
     9361 agatagaata ctataatgat gatgatttaa atagtattgt atcaatgctt cttgaataga
     9421 acaaaaatat aaaacagaga ggaagattgt tatgatttat ttagataatg ctgcaactac
     9481 atatccaaaa ccagaaagag tgtataatgc tgttttagat tgtatgaaaa attattgtgc
     9541 aaatccaggt agagcaggcc ataaacttgc tatgagagct gctagggaga tatatgatac
     9601 aagagagaat atagctaagt tgttcaatgt aattaatcct atgaatatag tatttacatc
     9661 taatgcaaca gattctttaa atttagcgat aaaaggtgta ttacaagaag gagaccatgt
     9721 aataacaacg tctatggagc ataattcagt aataagacct ataaaagcat tggaaaaaag
     9781 gggtattgaa aatactgtag taaaatgcga ttatgaggga tttttagatt atgaagattt
     9841 agaaaaatca ataaagaata atactaaatt gattgtaaca acacatgcgt ctaatgtatg
     9901 tggaacttta atagatataa aaaaagttgg agaaatagct aaaaaacata atatactatt
     9961 tttagtagat gcatcacaaa cagcaggtgt ttatgatata gacgtaaatg aatgtaatat
    10021 agatatgttg gcaatgccag gtcataaatg cttatttgga cctcaaggaa caggtatatt
    10081 atatgtaaga gaagggttga acttaaatat attaaaagaa ggtggaacag gtagtaaatc
    10141 agaagaaata gttcaaccag agttattccc agataaatat gaatcaggaa ctcataatac
    10201 tccaggtata gcaggtctta accaaggtat actatttata tttgaaagag gaatcaacaa
    10261 tataagacaa catgaagaag aattatgtca atatatgata gacaaattag aggaagtacc
    10321 agatataaag atatatggac caaaggacag taaaaaaaga gcatcagtaa tagctctaaa
    10381 tataggagat atggattctg gagaggttac tttcttatta gatagtgatt ataatatagc
    10441 gactagatca ggaatacatt gttcaccact agcacatacc actttaggta ctttaaaaca
    10501 aggtgcagta aggttcagta taggatattt caacacaaaa gatgagatag ataaagcagt
    10561 ggaagctttg aaaaaaattt caaaaaataa atagcatgag atttaataat atagaaataa
    10621 aaaaggggat atttcaaaat aaaaatttga atcatctcct tttttgtata aaaaagaaaa
    10681 ggggtcccta agaacctcta atctatttga taattgtttt aagaagttac tttaagtaat
    10741 ctccttcttc aagaactata tgaccacttt cgtaattaga accatcaaca gtaattttta
    10801 tttgttcaac attcatattt tttatgtaag tacgagcaac tgaatcaagc attaaagttt
    10861 ctgcatcact acctaagttt gactttgtaa agtcagagct aacatctaat ataccagttt
    10921 ttacaccatc aatgtctttt gtagtaaatg aatttaattt tgcagattct ggaacaactt
    10981 taagtttttg aagttcttca aataaagtgt tctcatcaat tttatttaaa tcaactttat
    11041 tttcaattaa atctgtctta tctacatcaa aagagtaaat agtagctgat tgagttttag
    11101 tttcttcttt tggagtaggt ttagctttgt tattaggaac ctctttttca gtattgtttt
    11161 cttcttttgg ttgctcaaca tttgtattgc tcttagtttc ttttttagat gaatcacttc
    11221 cattttgagc attagtacaa ccaactgcga atatagatat agataatata cttaacacta
    11281 acattatttt cttatttttc ataaaaattt ctcctctctt aaaaactatt cttatatata
    11341 attatagtcg ttatgagcaa atatatgaat acaaaatagt aacaaattta aaaaatgatt
    11401 tatttaaatt aataaaactc aaaataaact aaattttagt catttttatg ggaaatcatt
    11461 taatgtacag tgaaaatatt gtaaaataaa agtagtgttt ttaaaataaa tataagttat
    11521 aattatatta taaaaaaatg tcttcaaaag gtgggaatag tatggaaatt ttgagcttag
    11581 gagaaaagat taaaaaatta agaaaagaaa aaaacatgac attaaaagaa ttagctggag
    11641 ataggataac agcagcacaa ataagccata tagagaggga taaatctcat actagttatg
    11701 aactgttgga atatctaggc gataagctag atgtaagtgt agaatactta ttagagacca
    11761 aggaaatgca atcaaaaaag ataactgata atttaatact acagagcgaa atttatataa
    11821 aagaaaatga attaaacaag gctgaggacc aaataaaaga agttttagat atatgtaaga
    11881 gatacgattt aatggagaac tatggcagat gtaatttttt aatggctaat attaatttga
    11941 aaaaatctaa ttataatgat gctatagaaa actttgaaaa ggcattgtac ttctttataa
    12001 aaaatagtga tagtgaaaat atacttaagt gttatttaaa catagggaaa atatatatgc
    12061 aggaagactt ttataaaggt gctataagcc actttaattt tgcagaggaa attttgtcag
    12121 agagtaaatt tgaggatgtt aacatacaca aggaactata tagtaagatg gcatattgtt
    12181 acataaaact agataatcca aatatgtcat tgaagtacat agaaaagata aatgaaatag
    12241 ataatagaaa tgatagaaaa gaagatgtcg atattttggt gttaaaagca aataatatgc
    12301 ttgagattgg aaaatatgaa gaatcaagag attactttaa aaaagcacta aaaatacttg
    12361 agagtgagga taataaaact ggattagcaa atgtgtattt aacaatttgt gatgtttaca
    12421 gaaaaatagg tgaaacagat agagtacttg aatattcaca aaaggtatat gacataaaga
    12481 aaaatgatga agatgaatac atgatgtcag gattatttaa gataatagaa gcatatatag
    12541 ataaagaaga ttatgattta gctagaaaat attgtaagat agccttagct tcatcaataa
    12601 aaaataaaaa taaattcaat gaatataaag cattgagatt ctatgctaat atgtataaaa
    12661 atcagaatga agtaactctt gcaattgaat atctgattaa atgcataaaa atagtatcag
    12721 atttaggcaa caatagaata ttggccaatt tatatataga cttaggtcaa ttatattcaa
    12781 atatatcaaa agaaaaagag ttagaatatt atcaaaaagg tgtatttatg tacaaaaact
    12841 tagaaataat gtagggtaaa taaacctata agggtataga aacaatctat aacaagcaag
    12901 ataatatcta tgataaaata taaattgtat gaatattttt aaggagatga atcagacaat
    12961 gagtataaaa atagatgcga gagggcttgc ttgtcctaaa ccagttataa atacaaaaag
    13021 agaattggat aacctagaag aaggtgttgt agttacaata gttgataatg aaacagctaa
    13081 ggagaatata ttaaaacttg ctaaatcttt aagttgtgaa gcaaatattg ttgacgaaaa
    13141 agagaatttt atatctatag aaattagaaa aggtaaaaat atactagata aacaaaatgt
    13201 tgtagataat aaagaaagtg aattaaacaa tacatgtata tttatatctt cagataagat
    13261 gggacttgga aatgacgaat taggaaaagt gttaataaaa ggatttatat atacattaac
    13321 agaatctaaa ccttatccta agtatgtact tttagttaat ggaggagtaa aattaagtgc
    13381 tgaaaatgaa gaaaccatag aaaatctaaa aatacttgag aatatgggtg tagaagtttt
    13441 atcttgtggt acttgtttag attattataa tttaaaagac aaattacaag taggtacagt
    13501 tacaaatatg tatactatag ttgagacatt aaagaatgca tcaaatacca tttctatata
    13561 atgtatgtat gtttaataat ataaaactaa caataaagat taactcaaat agatagtcag
    13621 aaaggattaa gtaatgaatc aaatgtatat agtgtcattt aattcgacac atcatgcgat
    13681 aagatcagaa aagttatttg gtgaaaatag tctaaaagta atggctcttc caacaccaag
    13741 agaaataaca gcaagttgtg gaatatcaat taaattttct tttgaagata tggaaaagat
    13801 aaaaactatt ttggttgaaa ataatgtaga tataaaagga atttattgta taagtaaatt
    13861 agaaaatggt tcaaaagaag tagagaagtt agattaggag gctaattatt tatgccaatg
    13921 gatttaaaaa taggtgatat tgttgaatta aaaaagcaac atgcatgtgg gtgtaaagag
    13981 tttgaaatca taagaacggg aatggatata aagataaaat gtactaaatg ttcaaggcta
    14041 ataatgcttg atagagagac cttagaaaag agagtaaaaa agctaataaa aaagtgataa
    14101 aaatttaaca cttaaaaaat gtcaaatacc gtatattaat atccaaaatt ttggtataat
    14161 aagaattgaa gaaaacattt tcatgggggt gctttaatta tggcagttaa atatgcaaaa
    14221 agaatgcaag gattacaagg atcagaaata cgtgagcttt taaaacttac tcaacaacca
    14281 caaataatat cttttgctgg tggaatgccg gctccagagt tatttccagt tgaggaaatg
    14341 aaaaaagtat cagttgcagt gcttgaagaa aatggaagat cagctatgca atatactaca
    14401 acagaaggat atgaaccatt aagagaaaaa atagcagcta gaatgaatga taaaaacaaa
    14461 actaatgtaa acaaagatga tatattagtt acaagtggtt ctcaacaagg tctagatttt
    14521 gcaggaaaag tattcattga tgagggtgat gtaattttat gtgaaagtcc atcttacata
    14581 ggagctataa acgcatttaa atcttatcaa cctaaattca tagatgttcc aacagattct
    14641 gatggaatga taatggaaga attagaaaag atactagaaa caactgatag aataaaaatg
    14701 atttacgtaa tacctgactt ccaaaatcca acaggaagaa cttggccact tgaaagacgt
    14761 aaaaaattca tggaaatagt aaataaattt gaaataccag taatagaaga taatccatat
    14821 ggagatttaa gatttgaagg agagacttta ccatctttaa aatcaatgga tacaaaagga
    14881 ttagtaatat tcttaggaac tttctctaaa atattctgcc caggatacag attaggatgg
    14941 acttgtgctt ctccagaaat tttatctaag tttaactttg caaaacaagg tgcagactta
    15001 caagcatcaa ctatatctca aatggaagta agcaaattta tggatatgta tgacctagat
    15061 gcacatgttg acaaaataaa agcagtttat gttaaacgta gagatgtaat gcttaagact
    15121 atggaagaag agttcccaga aggattagta tttactcatc cagaaggtgg attatttaca
    15181 tgggtagagc ttccaagtaa tttaaatgct aaagaattaa tgccaaaatg tttagataag
    15241 aatgttgctt atgttgctgg aggaggattc ttccctaatg gtggtagaga aaatactttc
    15301 agacttaact attcaaatat gccagaagaa aaaataatag aaggtataaa aaatatagca
    15361 gctgtattaa aagaagctat gggtgtagaa gcttaattaa aaataataac ttatatataa
    15421 tacaagggat ggttgttgcc atcccttgtt tgtttgaaaa gtatataatt caccttatac
    15481 taaactttgt ccaaaattta aattgacaga aataagaata aatgataaaa tgtgtaggta
    15541 tgagatttta gtctcatttc tctgctctaa gagtgtagag ccgttaagtc caaagggagg
    15601 tgaattatag tgagaaatta tgaattagtt tatgtggtaa agccaaactc tgatgaagaa
    15661 gtaagagagg ctatactaaa caaagttaag gaagttgttg caactgacgg agaaatagtt
    15721 aaagttgata cttggggaac taaaaaatta gcttatccaa tagctaagtt tacagaaggt
    15781 ttctatgtat tagttaactt caaatcagca gtagacgttc ctaaagagat agacagaaac
    15841 ttaaagataa atgaaaacgt aataagacac atgatagttg ttgcttaata actggtttta
    15901 ttataaaaaa taggtggtgt ttttatgaat caagttgtat tagtgggaag gttgactaga
    15961 gaccctgaat taagatacat cccaggtaca ggaacaccag tggcatcctt tacaatagcc
    16021 attgatagag actatgtaaa aaaagatgga tctaaagaaa ctgattttat acctgttgaa
    16081 gtaatgggta aatctgctga attttgtgct aactacataa ctaaaggtag attagtagca
    16141 cttcaaggat ctataagagt agacaattat cagacacaat caggtgaaaa gagaactttt
    16201 acaaaagtaa gtactagatc agtacaagct ttggatagta agaataaatc ggaaaattca
    16261 tatagagaga gtgctccagc ttttgagcca agctttgaac ctcaaggttt agacccacaa
    16321 ggtttccaag ctatagatga tgacgatata ccattttaaa aaggagggaa aaaagtcatg
    16381 atgaataaaa aaagacgtaa gaaaaaaaga gtttgtcaat tttgtgctga caaaaacgct
    16441 aaaatagatt acaaaagtac tcaaagatta caaaagtata taactgaaag aggaaagata
    16501 ttaccaagaa gaatatctgg aacttgtgct aagcatcaaa gagaattaac agttgctata
    16561 aaaagagcga gaaatatagc acttttacca tatacattag actaaaaaaa tgagccttgt
    16621 gctcattttt tattatatgt atttatatat ctaaatgagg cgagctataa agctcgtttt
    16681 tttgtttata tacttaaata tattattatt tatgatataa tattatatat taaggagggt
    16741 tgtaaacgtg aaaatagata aaggttctga gataacgaga aacataaaga taatagaatg
    16801 gatgaaaaca gaaatattaa tgagtgtaag tgatttgttt aatttattgt ttaaaggggt
    16861 aaaaccatta gatgaagcaa ttcaagatac tttagcaaat ataataatga taacatatct
    16921 cttagcaaag agattaggaa ttagttttag agatgtggat tataaagtca aggaaaaaat
    16981 aaaaattggt atagatgagg accatagcgt tgaaagatgg tatggagatt tatctaattt
    17041 aaaaaaacat atggataata gggagtgata ttaaattgaa taataaaatt agactatcta
    17101 aggcaatgtc tataatagtt ttagctataa taatcgcttt agtaatagcc tatattccaa
    17161 tgctaggaat atttagtatg ttagcagctg ttccatatgt aattataggt gctattactg
    17221 atagaaagta ttctcttata tctattttgg ttacattttg tgtactgatt ctatttgcag
    17281 acatttctta tgcattaaat atatgtataa tgtattcttt accaggtata gtaataggaa
    17341 agatgctaaa gagaagcttt gaacaagagg acagtaataa attcgaaccg atatatggtg
    17401 gaaccataat atttgtactt tcaatgcttg tatatttttt tgtattaaag acatttttaa
    17461 atgtgaatct attagatgaa gtttctaaga tgatatcaga aattgtaaat attcaaaaaa
    17521 atagtttttc tgcaactgaa cttaaagtgt ttgataaaat gaagccagat gagattgtaa
    17581 gttattttac aaatatgcta ccaatgatgt tatttttaca agggttgtta tcagcgtttg
    17641 tcacatatta tttgagtgta ttttttataa aaaggattac aaagatgaat ataagatttc
    17701 ctaagtttgc tgatttttat ttaccgggaa atgctatact cacaactttc ttactttatt
    17761 tattagtctt atttattcaa attattggtt caaaattata tacagaatta ataatgacta
    17821 atttacagct agtttttaat ttaatgtttg taattcaagg tatagcagtt tgcatttatt
    17881 tcttaaagaa atggataaga cagggaccaa ataaaattat gtttttttca gggataattt
    17941 tatgtttatt tggatttatg ggaatatctt ttgttggtat ggtagatagt ataattgact
    18001 ttagaaaggt aagaagctat aaatccactt aggaggatag aaatgagtaa taaacaagcc
    18061 tttaaattaa atatgccaga aataaattta tatataattg ttattggtat ttctagtata
    18121 attttgcttt attacaactt gtatgtaggg tgtttatttt tctgtatttt tgtatatatg
    18181 gtttttcaca attggagaac tactaatatt agacgtcatg aatggacaga gtatattcaa
    18241 aatttatctt tagatataga tgaaacaact aaaaaagcca taataaatct tccaatacca
    18301 ttatgtattt tagaatttga tggaaatata tcctggtata atggaaagtt ttatgatatg
    18361 attggtcaaa aagatttact tgataaaaat atagaggata tagttaaaaa tcttaattta
    18421 agaaaagtat taaatgaaaa taaagagatg tacacagaaa taaactataa agaaaaagaa
    18481 tatacaataa tttataatgt tataaaaaat gatcaagaaa aaaatcctaa atatttgatg
    18541 atattatact ggatagacaa gactgaatat ttaaaagtaa aacaaaatta tgatgatgaa
    18601 aaaaatgcaa tgatgctaat acaagtagat ggatatgatg aagtattaaa aagtgcagca
    18661 gaagataaga gagctttaat taatgttgaa gtagagaaaa tactttctgc tttagaatta
    18721 aattcaaatg gtgcattgag aagaacttca aaagacaaat tcttcttggt aatgcataaa
    18781 aaagagttaa aaaaattgga agctgagaag ttttctattt tagatacaat aagacatatt
    18841 gattatggaa ataatcttcc tgttactata agtataggaa ttggtataga tggagataca
    18901 ttaaatgaga acctaaaact tgctacgggt gctctcgatt tagcacttgg tagaggtggt
    18961 gaccaggctg ttgtaaaaac aaaagataaa tttgtgtttt atggaggaaa atctaaagct
    19021 gtagaaaaga aaaccaaagt aaaatccaga ttaataggtc atgctttaag agaggttata
    19081 caacaaagtg accaggttta tataatggga cataaatatc ctgatatgga tgctatggga
    19141 gcagcagttg gtgtttatga tatatgtaaa tcctgtaaca aaactgcaaa catagtttta
    19201 caatctgtaa atgaatctat agaaatattt ataaataaaa taaatgaaaa taattactat
    19261 aaaaagttgt ttattggaaa agaggaagct attgacaact gcactaaaaa tacattagtt
    19321 gtagtagttg atactcatag accaaattat actgaatgtg aagaactgtt aaagctatca
    19381 gagaaggtag tagttataga ccatcataga agaggtgtag agtttataaa tgatgcagtg
    19441 cttttattcc atgagatata tgtatcctca acgtgtgaaa tggttacaga gttagttcag
    19501 tatatggatg aagatgtaac aataaataaa cttacagctg aagggctttt agcaggtata
    19561 agtctagata ctaaaaactt tgcattcaaa acaggtgtta gaacttttga agctgcttct
    19621 taccttagaa aagttggagc agatactata gaggtaaaga aattcttcaa ttctgatgta
    19681 aaagatttca taataaaagc tgaaataatt caaagtacta aaataattaa taatagaata
    19741 tgtttagcat attcaagtac tgagatagat agtataaatg ttataatagc tcaaactgct
    19801 gatgagttat taaatatcaa agaagtagaa gcttcctttg tattaggtga aaaagatgat
    19861 actatattta taagtgcgag gtctttagga caaataaatg tacatgtact tatggaaaaa
    19921 ttaggaggcg ggggacacat agatatagct ggagcacagt taaaaaatgt ttctctaaaa
    19981 gaagcttata agatggtaaa taaaataata gaggagtatt tggaggagga agaatagatg
    20041 aaagttatat tattaaaaga cgtaaaggga actggtaaaa agggagaaat gaaagaagta
    20101 agtgatggat atgcaagaaa tttcttattt cctaaaaaga tggctgtgca agcagatagt
    20161 gtagcaataa aagaattaaa tgaaaagaat aaatctaaag aaataaaagc acaaaaagag
    20221 tatgaagaag cagttttatt aggtaagcaa atggaagaaa ttaatataga aatatattca
    20281 aaagcaggag aaggtggaag attatttggt tcaataactt ctaaagaaat tgctgagcaa
    20341 ttaaagaaac aaaaagacat agatgtagat aaaagaaaaa tattattaga tgaaccaata
    20401 agatctttag gttcaacttt tgtagaaata aaaatacatc aaaaagtaac tactaaaata
    20461 agagtggatg taaaagaaaa acagtaataa ctagaggtga ttaagatgga agatatgacg
    20521 agaattcctc ctcatagtgt agaatcagaa caatcaatat tgggttctat acttctagat
    20581 aaagatgcta taataacggt tacagaaact ataaagcctg atgatttcta taaagaggct
    20641 cacaaaataa tatatgaatg tatgataact ttaagtaata aaggtgagcc tatagacttg
    20701 ataacattaa cagaagaact aagaaaacaa gggcatctta atgatattgg aggaatcagt
    20761 tatattacaa gtctttctac tatagtaccg acgacttcaa atgtaaaata ctatgctgat
    20821 atagtaaaag aaaagtctgt acttagaaag cttataaaag cttctaatga aataataaat
    20881 ctaggatata gtggagcaac taagattgaa gatgttttag aacaagctga aaagagtata
    20941 tttgatatat cacaggagaa aactagtgat gattttaaat ctataaactt agttttgatg
    21001 gatgcctatg atatgataga aaaattatac actaacaaga gtgatgtcac tggtataact
    21061 actggtttta aagatttaaa caaaaaaata aatggtcttc aaaggactga tttaatatta
    21121 attgcagcta gaccagcaat gggtaaaact gccttttctt taaacttggt tcaaaatgca
    21181 gccttaaaag gtgatgcctc tgtagctgta tttagtttag agatgtctaa ggaacaatta
    21241 gtacaacgta tgttatcttc acaatctagt gtagaattaa aaaaaataaa gactggaaca
    21301 cttaatgata atgattggcc tagaattata gatgctatgg cagtattatc agatgccaaa
    21361 atacacatag atgatactcc tggtataaaa atatcagaac taaggtcaaa gtgtagaaag
    21421 ttaaaaatag aaaaaggact agatttagtg cttattgatt atcttcaact tatggaaggt
    21481 gaaggtaata atgaaagcag acaacaagag atatctaaga tatcaaggtc actaaaaata
    21541 ttagcaaaag aattaaactg tcctgtagta gcactatctc agttatcacg tgcaccagaa
    21601 caaagagcag accatagacc tatgttatct gatttaagag agtctggagc aatagaacag
    21661 gatgcagata tagttatgtt cttatacagg gacgaatatt atcatgctga ttctgagagt
    21721 aaaaatatag gagaagtaat aatagcaaaa aatagacacg gtgaaactgg ttctgtggaa
    21781 cttgtttggc ttggtgaagt tcaaagattt ggggataagt tgagagacct ataatctaac
    21841 gaatatccac agaacgtcct ttctttctac aggaaaaatg tggatacatg tcactaaata
    21901 tgtccaaaca ttgaaattta gctaaaaaac tatccacact atccacagag agcctgttga
    21961 taagtgtgaa taagtagata aagcaagctt ttggcttgct ttatctgtgt ataaatatta
    22021 attattagga ggaataatat ggaaaaaata agcttaaaat atatatatcc caatataatt
    22081 aaagtacttg atgaaataaa cttatttaga gtaattgaca ataatttaag agaatctatt
    22141 gttgtttatg ctaataatgt ggataatcag tatcatataa acatgactaa tacaaacttt
    22201 ggaaacataa ttaacatatg taaattagaa aaacttttag atgtggataa atttatggaa
    22261 aaagttataa aatatgaaaa agaaataata gagaaagaag agttttcaaa aatagaagaa
    22321 tatatgttaa atattggaga atactaaaaa agttgaaaaa aatacgaatg tttgatagaa
    22381 taagtaagta aagtgttcga gagtatataa aatatagagg tgaattacat gaaaacagtt
    22441 gcaattgttg gttctcaatg gggagatgaa ggtaaaggta aagttataga ttatcttgct
    22501 actcaagcag atgtagtagt aagagggcaa ggtggaaata atgcaggaca tacattagtt
    22561 gtagaaggta aaaaatacgc attacatcta attccatctg gagttttaaa tccaaataca
    22621 gtgaatataa tcggaaatgg aatagtgttt gaccctaaag gatttttaga agaattggaa
    22681 atgtttaaaa cggataatat aagtacagaa aatataaaaa taagtgatag agcacatgtc
    22741 atattcccat atcataaaga actagatgca ttgtcagaag aagccagagg cgatttaaag
    22801 ataggtacaa caaaaaaagg tattggacct tgttatatgg ataaaacaga gagatctggt
    22861 attagaatat gtgacttaat ggataaagat aaatttgcta taaaattaaa agctcaaata
    22921 gatgctaaaa atgaaatagt taaaaatata tatggaaaag aagaattatt tgattttgaa
    22981 actatatata acgaatatct aggatatgca gaacaaataa gaaagtatgt ggcagatacg
    23041 tctgttattg tatatgatgc agtaagagca ggtaaaaaag tattgtttga aggagcacaa
    23101 gggactttat tagatttaga tttaggaact tatccatttg ttacatcttc tcatccaaca
    23161 tctggtggat ttgcaattgg tgcaggtata ggaccaaata tgataaaaga cgttgttggt
    23221 atagtaaaag catatactac aagagttgga gaaggaccgt ttgttacaga gcaaataaat
    23281 gaaactggag ataagataag agaacaagga catgaatttg gggtaactac tggtagacca
    23341 agaagatgtg ggtggtttga tgcagttata gttaagtatg cagctagagt taatggattg
    23401 actagtattt catttatgtt acttgatgta ttaactggat ttgataagat aaaagtttgt
    23461 acgtcttata aaatgggcga taagataata acggatttcc cagcttcatt agatgattta
    23521 gcaaaatgtg aacctgtata tgaagaatta gatggatgga atgaagatat aactcaaata
    23581 gataactttg atgaccttcc agaaaatgca aagaaatatg tagcaaaaat agaagaacta
    23641 gttggagtaa gtgtagatat ggtttctgtt ggtccaaata gagctcaaac tataataaga
    23701 agaaatatat ttgcttaata ttttatcaat taaataattt aaaatagttg aagaagagta
    23761 tctcaagata atgtttatag ttattagaga tgctctttta tatttgactt acattgattt
    23821 tttattaagt taaagctact tatacatatt ctaactgaaa ttaaagttga atttatgtag
    23881 atacatatag gttttttaaa aagaattttg ttgaaaataa atggctaaag atgattttaa
    23941 ttatattcta aaaaaagtaa aaaaaactat tgactaaaaa ttatatagac gctataatat
    24001 aacttgtgag tgaaagaaac gacgaaaaaa gtcgaaaaaa tgtggtctat tagctcagtc
    24061 ggtagagcac ctgactttta atcagggtgt cccgcgttcg agtcgcggat agatcaccaa
    24121 aatatggctc gttggtcaag aggttaagac accgcccttt cacggcggta acaggggttc
    24181 aattccccta cgagtcacca gtatgggact atagctcagc tgggagagca cctgccttac
    24241 aagcaggggg tcacaggttc gagccctgtt agtcccacca aattttgaat tagagataaa
    24301 tactagatta tatgctggtg tggctcaacg gtagagcagc tgacttgtaa tcagcaggtt
    24361 gtaggttcga ttcctatcac cagctccaca aagtacataa attcaaatta atataatttt
    24421 ctatgttaca ggatactttt aagtatcttt ttttaatttc atttttattg tattaaaaat
    24481 gaaattaaac atacaacaaa attttgaatt acttgcaatg tgatagtttt aaatataaaa
    24541 tttagaatca ttctagcata gtattatgta atatagcaaa tttaatttat agtatcaata
    24601 aatatagact actaagaatg taaatgattt gttagattaa gatacattag atatgtaaat
    24661 agaaaaataa aaagtatgtt ttaatttatg tgatataagt tttaaacaga gaagcaagtt
    24721 tgctcttacc tctctattta aaaataaaaa aatgtttttt aagggctatt ttatttaaat
    24781 ctttcccatc tcaaatcatc tccagtaaat tctaaaatcc taaacttatc taatattcta
    24841 gagagagttc tttgagtgta agtatttcct atttgagatg gtgttaagtt ggttgaaata
    24901 atgatttttt taccagcaac aagtcttgta tttacaatat tgaagatttc tccactagta
    24961 aaagaattat taagttcagt tccaaggtca tctattatta ataaatcaca atcaaacaag
    25021 tttttataat tatcttcact tatttgatta tttgtgtctc ttctaaattt ataatcttca
    25081 agaatatcta aaattctaaa agaagtttga tagattacaa cattgccttt atctaacaat
    25141 tcctttgcaa tacaattgca catataggtt tttcctagtc cagtagaacc ataaaacagt
    25201 agattttcac tattatcctt cttaaaatca tgaacaaaat tttcgcagat acttaaatta
    25261 tttagcatat tttctctagg agatatttca ccatcagagc ctttcttagg agaaaatata
    25321 ttcaagttaa aattcgaaaa attttcctga cttaaaatcc tatctaaatt agacatttta
    25381 taagcttcat ttacaatttc ttgtttgagg caagaacatt tttctccatt aggtaaaaat
    25441 cctttatctt tacaagaaat acattgaaac tttatttcca agtaatcaag aggtatgtta
    25501 ctttcagcta aaagtctttc tttctttact tttaaagatt ctatattctc tttagtcttc
    25561 tttacaattt catctttaga cttaggattt aaaagtacta tcttagctaa actgagacct
    25621 attttagaaa tttcatcatc tatagatttt atctcaggga ttctattata tacctcattt
    25681 tttctatgtt ctaataagag ttcattatca tctcttctct tagcatattt agcaagtatt
    25741 tttcttattt tatcctcatt catttaatca cctacttaaa cttttctttt tgactttttt
    25801 taataatctc atcgagctca tcagaggtat actgagtgaa tgtttcgttg aaattatgaa
    25861 acttagtttt tttgtaagta ttgttgtttt gagtagtatt aatctgcttc ttattaatat
    25921 tctccttaac tatacgttct tcctcttttt gctttaaatc atttaaattt tttatatttt
    25981 tttcattcca atttttaatt ataccattta tataagatat acttgggttt gaaatattct
    26041 tagacttaga acatgcattt attatcaaat ctatatccat attaaattta tcaaaccaag
    26101 tgtccataag ttctttttct gattttgatg gttgtcttga aaatcccaat tcattgaaaa
    26161 tcgttttata taaaatatat cgttcacttc ttaccaaaaa gctttcttca acatcttttg
    26221 gcgtatataa atttgaatca taccaatttc taataatgcc ttcaatgtat ttaactggct
    26281 ttgatgtacc agttttatct ttgacgtatt catatgcgca aagtatcata tcggggctca
    26341 tattgtattt attcattata tccataatac tcatcttttc gcttgggtcg agatatcgcc
    26401 caactatttt atttatagaa ttaaacattt ttctaatact tggattttca gaagtagata
    26461 caaccttatc tgtattagat tttatagatg aattattgct taatatattc tcaatataga
    26521 aattttttaa atctaaaaat tctattgaat aattaaaatt atcatattct ccattgtcat
    26581 gtatttttat aatcttcttt tcttcccaga atttccaagc agataacaca tcagacaatg
    26641 gtatatttag gtttttggct attgaattat tgtcgaactt aggattagaa gtaatatcac
    26701 atgcttgtct atatcctaaa agataaactt ttacgtataa cccgtccgcc attggcataa
    26761 atatatctat aaagatattg ggaatagtga tttcacctaa atctatttca ttgctttctt
    26821 taaaaaacat tttatcacct cagattaaat tatatcattt tttatattat atttgtctgt
    26881 aaacaaataa aaggtcacaa gattagcatg taaatatttt ctaaaagtat tcattttgaa
    26941 aaggaggaga tattttggtt ataaaattca accaaaaagt aagaagaaca ttatcagtag
    27001 cgattctatt tatggtatta ataataggtt gctttataga aaaggatgac agtttagaga
    27061 catacaaaga aaaaactaat tcaaaaataa atcagtataa tatggatgtt atatttgatg
    27121 atgagactaa gaggttaatg tgcaatcaga atgttgacta cataaataat acaaaatcaa
    27181 acttagataa aatatatttc catatatatc cgaatgcttt ttccaagaaa gattttgctc
    27241 cttttgaaaa aagtgaaatg acaagggctt atcctaatgg atttaacgaa ggatatatag
    27301 atataaagaa tatattaaat aataatagta aaatggagta taaaattaaa ggagacaaaa
    27361 atgacatatt agaaatagaa ttagggaaag agttaaaacc aaatgaaaga atgtcattag
    27421 atataaaata taacgtaaaa ataccaaatt caattggaag atttggatat ggagaaaata
    27481 ctataaatgt tactaattgg tttcctatag cttgtgtaaa tgatgaaagg ggatggaatt
    27541 taaagagtta tgaaactatt ggcgacccat tttatagtga gactagcgat tttcatgtga
    27601 aactattgat tccaaataaa tataaaattg ctcatacagg aaaacttatt gatgataaac
    27661 atgataataa gaaaatgcta tatgaaatag aagcggaaaa cgttagagat tttgcattta
    27721 tactaagtag caaatttaat gtaaacaaag tagattcaaa gggagtagct atcaatacat
    27781 ataatttaaa taaaaagctt tctaaaaaag caactgaagt ggcaaaagat tcaataaata
    27841 tatttagtga attatttgga aagtatcctt acaaagaatt ttctgttgta gcaagtgact
    27901 tttttatagg ggggatggaa taccctatgc ttgttatgat agaccaaagt ttatataatg
    27961 aaaaaaatga atttttacta gaatatgtta ttgcccatga gacagcacat caatggtggt
    28021 attctgctgt tggaaatgat gaaataagtg aaccctggtt agatgaagca cttactgaat
    28081 attctacaat agtttatttt gaacaaaagt atggtaaaga tatggcaaac aagcttataa
    28141 aaactatgga gatacagaca aaaagttatt tgagcgaaaa tatatttaag ccagcaaatg
    28201 agtataaaaa ttctactgaa tacagcttga atgtttatac aaaaggcgca atagcattta
    28261 atgaaataag aaaagaagtt ggagataaag tattctttga gactttaaat gagtactata
    28321 ataagtataa acataaaaat gctaatggaa gagcttttgt ggaactatgg aatagtaaag
    28381 gtgtagatat aaataagatt ataagtgaat gtaagtaaac agtgaatgta agtaaatttt
    28441 gtgcagagta ttaaatttta tttaatactc tgtttttttg gtataatata atttataagg
    28501 cagaaaaaga ggtgaaggaa tgctaaagta ttgttcagta gggagtggta gtagtggtaa
    28561 ttgccactat atagggtata aaaatactaa tatactagta gatgcaggat taagtggcaa
    28621 gagaataact acaggtctta aagacattgg tgtagatgca gataaattaa aaggaatatt
    28681 tataacacat gagcatgttg accatataaa aggagcaggt atattatcaa gaaagtttga
    28741 tataccagta tttgctaata taaaaacatg gtgttctatg aaggacaaat taggtgacat
    28801 caaagataaa aatatgaaag tatttgaaaa tgataaaacc tactcattag gtgatatcat
    28861 agtaagacct ttttcaatag aacatgatgc gtctgaccca gttggatata atttttatac
    28921 agagagtgat gaaaaaatgt ctatagctac tgatatagga tgtataactc aaaatataaa
    28981 aaaacatcta tataaatcta aattagtagt attggaatca aattatgacc ctaatatgct
    29041 tatgatgggt tcatatacat atgctttaaa gaaaagagta atgtcaagta ctggtcattt
    29101 atccaatgaa gatgcagcta atttctgcgt tgaacttatt aatgaaggta cagaatctat
    29161 attattagca catttgagta aagaaaataa ttttcctgag ttagcgtatg agacttcaaa
    29221 aggagtattg gcatcaaatg atatagtagt tggacaagat gtaaaactag atgtactatc
    29281 acgaaatgat atttctaatg tttatgagat gaagtagagg tgaataactt tgctaaagag
    29341 aaattttgaa aatgttgagg ataataaaat tttatactct ataaagcagg gcatgatttt
    29401 agcgatacct gctattatga ctggttcaac ggctcttgta attttgaatc ttccaataaa
    29461 ggcttatcaa gaatatttat caggcttgtt taatggagag gttacaaata ttcttaactt
    29521 tataaatgat tctactttag gaattatttc attaataata ttattaacaa taagctatag
    29581 ttatggaaaa atatatggga gcaagtatac agtacttgtt cctatagttg caatgtgctc
    29641 ttttttagta ttttcgcatg gtaatgagct taattcttat atagaaatat ttaagacaaa
    29701 gtggttattt acatctataa ttgtttctat gacatcttct atattgtttg taaaacttac
    29761 agaatctttt gttacaagtg taaaatttca tacagaaggg gctgatgcag actttaatat
    29821 ggtagtttcg gctatagttc ctttcataat agtagtattt ttgttttcta tttttagagt
    29881 gataatgata agtctaattg gcagttcaaa ctttcaagat atattttata atttattttc
    29941 aaatgttttt aataaaatgg gaacaaacct tatgagtgct ctgttgttta tttttttaat
    30001 gcactttatg tggttttttg gaattcatgg tagtaatgta ttagataccg tcgcaaaaaa
    30061 tttatttgaa aatagtatgg caattaacat aaatttagtt aataataatc aattaccaac
    30121 agaaattttt actaagacct tttttgatac aatggtttta ttgggaggat gtggctcttt
    30181 actatgttta gtaattgcaa tttttcttag tgaaaaacgt ataaatgtaa gaaagttagc
    30241 taaaattgct tcaataccag ctctttttaa tatcaatgag atgctagttt tcggtatacc
    30301 aattgttttt aactatatta tgtttgtacc ttttgtaatt actcctataa tcttaagcat
    30361 tacaagttat gtggctatgt ctacaggtat tgtaccatgt acagtttcag ctgtagaatg
    30421 gacctcacct atatttttaa gtggatatat gtctacaggt tcaataagag gtagtttgtt
    30481 gcaagtattt aatttatgtg ttggtgttat gatttatatt ccgtttataa aaatgtcgca
    30541 aaatagatat acatatatgt ttaaaaataa tatagaaaat ttaacaaact tattaaaaag
    30601 gtatgaatta acaggtgaac agcctaatct tctaaaccat aatggtaaaa taggttcaat
    30661 ttcaaaaatg ttggcatcag acttgaagtt tgctatgaaa agaggagaaa tagagctttt
    30721 ttatcaacca caagtcaatt ataatggaaa tgttgtggga gcagagggat tacttagatg
    30781 gaaacattca ataggagggt tcatttatcc accactagtt ataattttgg ctaaagaaga
    30841 agggttctta gatgagcttg gagagtatat tattaataaa gcatgtattg atttaaagaa
    30901 gagtgaaaag ctattgagaa atcctgttaa gttctctgta aatatatctc cagaccaact
    30961 tgatgaccct aagttaccag agcaaataaa aaatatcatt agtagtaatg atataaatcc
    31021 taatatgtta ggtatagaaa taacagagca aattgcatta tcaggttcac aaataattat
    31081 tgaacgtatc aatgcgatac ataacttggg aataaagtta attatggatg attttgggat
    31141 ggggcatagc tctcttatat atcttcaaaa taataacttt gatatagtta aattggatgg
    31201 ttcattagtt aaagagatac ttacaaataa aagaagtagt gaaattataa tgactattgt
    31261 aaatctttca aaaaatttag attttgatat agttgttgag tatgttgaaa atatagacca
    31321 aagggataag ttatatggtt taggatgtga aatatatcaa ggatattatt atagcaaagc
    31381 aatacctttt gaagagtttg tagaatatgc aaatcaagaa agagagatat aattttataa
    31441 gagcttgtaa attctagtaa tgcatagatt tgtaataaaa ttaagtttag gagaaatata
    31501 tgaatataag tatagtagta gtaggtaaaa ttaaagaaaa atatttaaaa ttaggtattg
    31561 atgagtttaa gaaaaggctt tctaagtatt gtaagttaga aattatagag ttggatgatg
    31621 aaaaagctcc agagaatttg agtgttaagg aaatggaaat tataaaatac aaagaaggaa
    31681 agaaaatttt agggaagata aagcataata gttatgttat agccttggct attgatggta
    31741 agggtttatc ctctgaagag cttgctaaga ctatgagtga tttagcagtt agaggtaata
    31801 gtagtctctg ttttataata ggaggttctt tagggctttc tgatgaggtt ttaggaaggg
    31861 ctgattatag gttatccttt tctaagatga cttttcctca tcagatgatg agattgatat
    31921 tgttagaaca gatttatagg gcttatagga taaataatgg agaaccgtat cataaataaa
    31981 tgatacggtt ttttttagtt tgcaacttaa taatattaaa ataacaaaaa agtattgaaa
    32041 gtatattgca tttaaggtag aattaaataa aatattataa cattataatg tttatatagt
    32101 tgttaataaa aatttggagg aaaatatatg ataaaaataa tgttggcctg ttcagcaggg
    32161 atgtctacaa gtttattagt tactaaaatg gaaagtgcag ctaaagaaaa tggtatagag
    32221 tcgcaaattt gggcaatacc agaatcaact atacaaaatg aaatagaaaa atgtgatgta
    32281 ttattactag gaccacaagt tagatatgta ttacctaagg ctcaagaaat tgctaagcca
    32341 tataatatac cagtagaagt tataaatatg atgcactatg gaacagttaa tggagaagct
    32401 gttttaaata gagcgataga acttaacaac actaagtaat ataaattata atgtattttt
    32461 aatatataaa ttgggagaat caaaggatgg atgaaaagat tatagaaata tcttttaata
    32521 taataggtta tgcaggtgaa ggtaaaggat tagcttttga agcaataaaa gaggctaaga
    32581 atggaaatat agaaaaagca aaagaattat taaaagagtc tagggaagtt ataaataaag
    32641 cacatagata tcaaacagaa ttaatacaaa acgaagctag tggaaacagg actgaaataa
    32701 gtgtcatact tatacatgca caggaccacc taatgaatgg tatgaatttt caacagttag
    32761 ctgaagaaat cgttgattta catttgaaat tacaagataa ataaaaatta aagataataa
    32821 attaagagag ttcttaaaag atacattatg tgtagcacac taattgttag attattgatt
    32881 caatatttag tgagtaatac attaggatta ttttgaggac tctttttata tttagtttaa
    32941 agttaataaa tatttattat taataatata agattatatg ttataatatt ataacatata
    33001 atttaagagg tgaatcatgt taagaaatca accattatat atacaagttg tagatatatt
    33061 aaagcaaaaa atattaaata acgaatataa aattgatgaa aatattccaa cagaaaggga
    33121 gttagagcaa gagtttggag tcagtaaaat tactattaga aaagctattg agatattaga
    33181 atcagaaggg tatgtagaga aaaaaagtgg aaaagggaca aaagtaataa gtaatagcct
    33241 ttttaataag ctatcaaaag cagagtcttt ttcaagtata cttttatcta aaggtcataa
    33301 gttatctaaa gagactattt caattgaaaa aataataaat gatacaaatt cacaagtatt
    33361 tagtatattt ggagaaaaat gtagcaagat tactagaatg tattatttag atagtaagcc
    33421 atatatattt tttactcatt atattccttt tgatgaaaat ctaactgttg aaagttttaa
    33481 taatgaaagt tctttatata tgtacttata taggagaaat ataaatataa ctagatttga
    33541 agatgaattt tttatagaag aatcagaact agaggtatca aaggctttaa atctagctaa
    33601 agacatatta ttaggaagaa aaagaaagac ctatgatgag aatgataata tagttgagct
    33661 atcatttgca agatataata cagaagtaca aaactatgta attaaatacg aaatataata
    33721 agaatcctga gtttattgaa atgataatat gctcaggatt tttgtttagt tgtaaaaata
    33781 aaaaatatta taacattata atgtttacaa ccaaacatta taatgttata atatttttaa
    33841 taaacaagtg atattatcaa ttacataaaa attagtttat gttatgaaat cgttattgca
    33901 ttataaataa aggataatat caataataca gggggataac tatggaaaaa ttcgcaagtt
    33961 ttttagagcg caagattatg cctgtagcag cgaagataag taatcagaga catatgagag
    34021 ctgtaagagc aggtataata gcaacattac cactaacaat agtggggtca tttttcacaa
    34081 tattattaaa catacctatt gatgcttata tggattttat agctccattt aaagcaatat
    34141 tagatatacc atttagatat actgtaggat ttttatctct atatgctact tttgggatag
    34201 catcatcact tgctaaaagc tacgatttag atacaacagg tgttggtatg cttgcagtta
    34261 tggcattttt agtttctaca atagttccaa ctcaagtagt tgaaccagta ggtggagtta
    34321 tagaagctgg tagatggatg gatatggcaa aacttaattc gccatcatta tttggagcta
    34381 tcgtaacatc gttaattaca gttgaaatat atcgttttat gaaggaaaag aatattacaa
    34441 ttaagatgcc agcaggagtt ccagtagagg tttcaaattc atttactgca ttattaccga
    34501 cagcagtaat tttaatgtta ttctgggtaa taagacatat tttaggattt gatataagtg
    34561 ttgcactaag cgctatgtta tcacctttaa aaggattctt atcaggaaat agtttaggtg
    34621 gtgggttact gacagtattc ctaataactt tattctgggt acttggaata catggtccag
    34681 ctataatggg gccagtaata agacctatat gggacatgtc tatagcagaa aatataactg
    34741 cattcacaga aggagtatca gctcatgcaa tgccaaacat atttacagaa caatttttac
    34801 agtggtatgt atggataggg ggagcagggg ctactcttgc attagttgta atgtttatgt
    34861 tttctaagtc acaatatcta aaaagtttag gtagattagg atttttacca gggttattca
    34921 acataaatga acctataata tttggagcac caatagttat gaatccaata ctagggatac
    34981 catttataat tggacctata ataatgacaa ttctatcata tgttcttact attacagggg
    35041 ttatacctat gatggtagca agattaccat ttgcaatgcc agcaccaata gcagcagtaa
    35101 tgagtactaa ctggagtata ctagcaggta tcttagtatt aatcaacttt gtgatatcat
    35161 ttgctgttta ttatccattc ttcaaagttt ttgaaaaaca acaattacaa agagaacaag
    35221 aagaaatgag tgaaaattta taaaaataca tagatataga ggttttgtac tatataaaat
    35281 atagaaaaag cttataagtt attaagggtg aaggtaggag gaatgacctt tgcctttaat
    35341 tttatatatt ttaaataatg taatttgagg aggagaaaga tggaaagaag attaggaata
    35401 tcagtatatc cagagcatag taacatggaa gatgataagg catatataaa aatggcttca
    35461 aaatacggat ttaaaagaat atttatgtgc atgctttcaa ttacaagacc taaggaagaa
    35521 gttaaaaaac attttaaaga aattataacc tatgcaagag aattaggatt tgaagtaatc
    35581 cttgatatag ctccaaacat atttgatata ttacaaatat cttatgatga tttaacattt
    35641 tttagtgagt taaatgctag tggaataagg cttgactatg gatatgatgg aagtaaagaa
    35701 gctatgattt ctttcaatcc atatgattta aaagtggaac taaatatgag taatgatgta
    35761 gcatacttgg ataatataat tacttacaag ccaaatgtaa ataatatata tggttgtcac
    35821 aatttttatc ctcaaagggg tacaggcttg ccatatgatt tttttgtaag atgttcaaat
    35881 agatttaaaa agtatggaat aagaactgct gcttttgtta attcaaaata cgcaacaata
    35941 gggccttgga atataaatga tgggctgtgt acgcttgaaa tgcatagaaa cttagatatt
    36001 gctacagcaa ctaagcactt attcgcaaca ggattgattg atgatgtaat tgtagggaat
    36061 gcatatgcaa gtgaagatga attaaaaaga atgtctgaag taaatgaata ccaagttgta
    36121 tttaatgtag tagctgaaga aaaaacttct gaagtagaaa agacaataat ggtaaatgag
    36181 caacacttta gaagaggaga tataactaat caggtagcaa gatctacaga agtaagaaaa
    36241 aaatatgcta aagaaaattt ccctaaacat gataataaga acctctttaa aagaggagat
    36301 gtagtgattg gtaataatga ctttggaaag tataaagggg aattacagtt gatacttcaa
    36361 gattgtacag atgaaagaaa aaataaggtg ggtagaataa aggaagacga aataatactt
    36421 ttagacttta ttgaaccttg gagtaaattt aagattattt tagagggaga gtaaagtgaa
    36481 gatagacata ttaataaaat gtggaaaaac agtagataaa agtataattg atatagctat
    36541 attagacgat aaaataatag aagtaaaaaa tcagatagat gattctaagt atcaggcaaa
    36601 acaaattata aacttagatg gagagaaatt tattagtgct ggttggatag atatacatac
    36661 gcattgctac gaaagactag acctttataa ggattatcca gatgaggttg gaataaaaag
    36721 tggagttact actgtagtag atgcaggaac aacaggagct ttagatatag gtaaatttta
    36781 tgaagatact aaaaaatata aaacaaatgt atatgcttta ataaatattg ctaagcaggg
    36841 aataacatct caagatgaac tttcaagtat gatgaatatt gatgaatatg aagttaaaag
    36901 agcagttaaa aaatacaaag attttgttgt tggaatcaaa gctagaatga gtaaaagtgt
    36961 tgtaatttca aatgatgttg aacctttaaa agtagctaaa agaataaaaa atgaacttaa
    37021 tctacctatg atggttcact ttgggagttc tcctccaacg atagaggata tatttgacta
    37081 tatggaaaaa ggagatatat taacccatat atataatgga aagccaaatg gaatacttag
    37141 aggcaatgag gttaaaaaag aaatcataga agctagagaa agaggtataa tccttgatgt
    37201 aggtcatgga accgaaagtt ttagtatgga tattgcaatg aaatctaagg atgcaggaat
    37261 ttttccagac actataagta cagatatata tattaaaaat agaataaatg gtccagtgta
    37321 taatctatct acaacaatgg aaaagtttat atatatgggc tattcacttg aagatataat
    37381 agataaagtt actaaaaatg cagcagatgc aataagttta aaaaataaag gtttaataaa
    37441 agaaggatat gatgctgatt taactatatt tgatgtggtt aatgaagaaa aagaacttca
    37501 agattctttg aataagtcag taatcacaag tacgtctata aagccaagag cagtagtagt
    37561 caatggagaa tatttaaata taggtaagtc tataggagag aatgagtaga agggagaaac
    37621 tattgtatga gtagtatatt tgaaaaatac tctgtaagac gtgttataaa tgcttcaggc
    37681 aaaatgacta tactaggagt atcaacagta gataaggaag ttattgaagc aataggtgaa
    37741 gggtgtaaaa atttctttat aatagaagaa cttataaata aaacaggtga atatatagca
    37801 aatttattag ggtgtgaaag tgcactaata gtttctagtg catctgctgg gattgcacag
    37861 agtgtgggag caatgatagc aaaagatgat atgagtctag tttacaatgt aaataatcct
    37921 aagaaacaag ttaaaaggga aataataatt ccaaagggac ataatgttga ttatggagtt
    37981 ccagtagaag taatgataag acttggagga ggagaagttg ttgaggccgg ttatgctaat
    38041 atgtgtaaca aggaacatat aataagagag ataaatgaaa atacagcagc aattatgtat
    38101 attaaatctc atcattgtgt tcaaaagagt atgctaagtg tattagatgc agtacaagtt
    38161 gctaaagaat ataatttacc tttgatagta gatgcagcag cagaagaaga tttaaatgta
    38221 tattatgaat tgggagcaga cctagtgata tacagtggtg ctaaggctat agagggtcct
    38281 agctcagggt tagtaatagg taagaaaaaa tacatagata atgtaaagct tcaatcaaag
    38341 ggtataggaa gagctatgaa aataggaaaa gaaaatattg taggtctaac atgtgcaatt
    38401 gagagatata taagtaatga taaagtaact ttaaaagaaa tggaggacaa gctaaatcca
    38461 tttatagaca agataaatac tataaaggga gtgagtgcta gtataactag ggattcagca
    38521 ggtagagaaa tacttcgtgg agaaattgac tttaatgaag atataataaa taagtcaaca
    38581 caacaaatta taaatgaact tagaagtgga gagatagcta tatacactag agactataag
    38641 gctaatgaag gcaagattga gattgatata agaagtgtaa ctggaagtga tttagatact
    38701 atttataata ggataaaaaa tatagtagaa agttagagtg tagagtaagt gacattataa
    38761 tgatttaata aattaaattt taccaatgga ggggataaat atgaattgta taccacaata
    38821 ttataaaaat agagtttgtt taaatgttct tgcaggaagc ataaaaaatg ctaaagaagt
    38881 atatgatgct tgtgacgggc atgtattgat aggagtacta tcaaagaatt acagtactgt
    38941 agaagcagct attgaagata tgaaaaaata ctcaaaggaa atagataatg caatatctgt
    39001 ggggcttgga gctggagacc caaatcagtc aaatatggta tctcagattt ctaaagaact
    39061 tcagccacaa catataaatc aagtgtttac aggtgtggct acgtctagag cattgcttgg
    39121 gcaaaattat agtataataa atggattagt ttctccaact ggtaaaccag gatttgtaaa
    39181 aatatccaca ggtccattaa gttcaaaaga aaaagatggt atagttccta tagaaacagc
    39241 aatagctatg atgaaagata tgggaggaag ctcaattaag ttttttccaa tgggagggct
    39301 taaaacaaag gatgaatata tagaagttgc aaaagcttgt gcaaagcata atttttattt
    39361 agaaccaaca ggaggtatag acttagataa cttcaaagag atagttcaaa ttgcacttga
    39421 tgctggtgta gaaaaagtta tacctcatgt ttatacatca ataattgata aagaaacagg
    39481 tgaaacgaaa gtggaagata taggagtact ttataaaata atgaaagaat tattgggata
    39541 gatttatgaa tatattagct tttggtgaaa taatgatgag actttcagta cctgactaca
    39601 agttcttaac acaaaccaat gaactaaatt atataattac aggcacggga ttaaatatat
    39661 tatctggact taaaaacttt ggatacaata cctatatgtt gacaaagttg cctaataata
    39721 atgtaggtaa agcatccagt gcaaatataa gaaagcttgg agtaaaagat gactttatca
    39781 catatggagg gaatcatata ggagtgtatt ttcttgaaaa tgggtatgga gaaagacctt
    39841 ctgaagtgac ttatttgaat aggttaaata gttcattttg tgagagtaaa ttacaagatt
    39901 atgatattga taaatgttta gaagaaatgg atatagtcca tatttgtggt attgctatgc
    39961 aacttacaga ttcagttaaa gagattgctt tagaactagc tagaaaatct tatgaaaagg
    40021 ggataaaagt attctttgat tttaatttta gacaatcatt aaatccagaa aggacttatg
    40081 atgatttagt aaatgattat aagaaaatac taccatattg tcatgggctg tttgctagtt
    40141 atagagatat aaataagata cttaatatag atagtctaaa tataaaatct agttcagaat
    40201 tgattaaaaa agagtatgat ataggtattg tagctggaac tatgagaaga tttgatgata
    40261 tgaataatag atttataaag ggatatatat caaatgatga tggttatttt gaaagtaggg
    40321 agtataagtt agaaatatat gacagagttg gtgctgggga tggatatgtg gcaggaatta
    40381 tatattcata tttggaaaat atgaagagtg aagatatagt tgagtttggg gttgctagtt
    40441 cggtgttagc tcatacaact tatggggata attctttagt aagcacaggt catgtactta
    40501 gactgatgaa caatgaaaaa atagatatat tgagataata catttgtata taataaaatt
    40561 tttatctttt ttagacacat ttttgacttg cttatatttg ttaaacagag ttatttttaa
    40621 cgattataaa tattaaatta aaagttaaat aataaaatag tagaaaatag cattaactat
    40681 agaaaaaatg gttaatgcta tttttatcgt taatattgat taattttata ttatagaaat
    40741 gaacttttct aatgctggat ttgtattttt tttattccat acaactacaa cttcaattct
    40801 attttctact tcttttatag gtactattgc aatatcacgt tctatcattg tatctgtaac
    40861 acaaaatgaa ggtaaaatgg atattcccat gttagaagat accaatgatt caatagtatt
    40921 aagattattt gaacgttgta ccacattagg tacaaaccct gcttcacgat atctattctg
    40981 tatcattgac ttttgtccat actcatcccc tgtttctttt acaaaaataa atttatcatg
    41041 ttgtaattca attaaatcga tagaatcctc attgtaaagt ggatgaccag gaggtaaaag
    41101 agctaccaaa gaatatcttt ctacagtttt atattcaaaa tcttttaatg gttgattttc
    41161 tggattaata acaaaagcga ggtccataga attatttttt actaagttta aaagttcggt
    41221 tatctcatta gtacaaatat ccatagatat attagggtat gtgttagaaa attcttttaa
    41281 atattgttga agtttgtttt tttcatatcc tattaaggtt ccaatgctca aagttccaaa
    41341 aaaaccatgt gcaaattgtt gtgtttttgc aatagcatca ttgatatttt ttattatagt
    41401 acgtgcctca gataaaaaaa cttttccagc tggtgttagt tcaacatgac gtttacttct
    41461 tgtaaataga gttacattta tttgttcttc taaagctttt atttgctgtg taacagcagt
    41521 ttgagaaata taaaattttt ctgctgtttt ggtaaaactt agattttctg ctacagatat
    41581 aaaatattct aattgtttag tattcaaata atcaccccta tttaaacttg attgtttgta
    41641 aaccttattt atctataatt aaagcttatt atagtataag aaaatttact tttaaagaat
    41701 attagaaaat atttgtataa aaatatatgt tttaagtata tctttacatt agattttata
    41761 aatttattaa taagaataac ttatatattc caatgaaaac gatattggat ttaagtaagt
    41821 ttgtctcata aaatgaaagt atatcaattg ataatacttt aaataagaag ggagaaaaaa
    41881 tggaagataa attttatgca aaaggcaacg gaaataacgg atatattaaa aatcttgaag
    41941 tttgttcttt taataactta gatggaactt gtggaatgtt tcaaatggct ctgtacaaaa
    42001 gagatgaaaa atactattta tatggatgct gttttggagg aaataaaaaa aatggagtaa
    42061 tgattagcga tattacagac ccttataatc cacaatttat aaaacatttt caaatgttag
    42121 accctaaaga gtatcctaca acaacaactc ccaaaattca aatagcagat gatttaatga
    42181 tagtagcaat gagttgtgga agtggaccag gagcacttgt tgaccaagct aaattagcaa
    42241 atattaagtg tgaagcagga attagaatat acagtttaaa agaagaccct ttaaatccta
    42301 agtttttagg atattgggat tgtggcttaa agcatgtaat gggtgttcat agattcatgt
    42361 acaatggtgg aagatatgta catttatcaa gtgattgtgt tggctttgaa ggtctgattt
    42421 atagggtcat agatataata aatcctacta atccagtgga aataggtaaa tggtggagac
    42481 cagaccaata tgcagatgga tatccaaata gaacttttga tgcaggagca cctcattgcc
    42541 cagaatttat ggataaagga tggcttcatg gacctccatt tgtaagagac ggaaaagcat
    42601 attgtggtta tggaggagct ggtttagttg tattagatgt tgaagattta acaagaccaa
    42661 gatgcttagg tgaattgcca tttacgcctg cattttctag tagacttgca ggtgcaagaa
    42721 ctcatacagc attaccattg ccaggaagag atttagtcgt tgttcaaaat gagggagaaa
    42781 gattccagtt ctttaaacca gataacatta cagatgttca agctatgaat aatatacata
    42841 tggttgatgt tagtgaccca acaaaaccaa cattaattgc tcaatttcca tatcctgaag
    42901 ttccaaaaga tttcccttat cctaacttta atgttgcggg attaggaaaa ccagggccat
    42961 ttggtccaca taatcttcat gaaccaatgg ataataagcc atggttagag caaagaggag
    43021 atagagtata ttgctgttat ttccatgcag ggctaagggt ttatgatgta tcagacccat
    43081 attatatcaa agagctagca tattttatac caccaaatcc aaataaaaca ccagaagaat
    43141 cttatttccc aggattccca ggaccacgct tggcagtaac agaagatctt atcgttgatg
    43201 atagaggcta catcatcata gatgctttag atgatggatt ctatatatta aaaatgaaag
    43261 aagattaaat aaatactaaa gagggagagt tgaaaatgaa aatattaggt atttcttttg
    43321 gaacaaaaaa tggaaataat gacacaatgt gtaaagttgc acttaaaggt gccaaagaag
    43381 caggggctga agtagaattt attcaaatgt catctattaa cattaaacat tgtactggat
    43441 gttgtgcgtg tgttaaaact ttattatctg ggaaaggtag tatgtgtgta ttaaaagatg
    43501 attttgaatg gttgcttgac aaaatgaaag atgcagatgg tatagtagtt tcagacccta
    43561 tttttgaaga aggggcatca ggattattcc atactataat ggaccgtttt ggacccagag
    43621 cagatagagg aaataacatt ataggaacaa aagttgcaga agctatagga ggaaaaattc
    43681 cagattcaag aatgcttaat gataaagtaa tatcatttat gggaatagga ggttctgact
    43741 ggggtacaag agttcaatgt gaacatgcta tgctagctct cataccaatg tggaaagtta
    43801 tagataatgc ttggttccca tgggcaaagg aattagtaat ggatgataca aggcttgttc
    43861 aagttcatca aattggttta aatatagttg aagcagcaaa agattttgaa aaagcttcat
    43921 atcaaggaga agaaggtgta tgtccacatt gccacaatag attattctac cttgagccag
    43981 gtactaaaaa ggcaatttgt gctctatgtg gtatagttgg agagttagat actataagtg
    44041 gaaaaactat atttagtttt ccagaagagc aattaggtca tgctcatgat actttaccag
    44101 gtaagtttat acatggtgat gatattaaga aaatggaagg tcattatgca gaagttcgta
    44161 aaacacaaga atttaaagaa agaaaaacat cttataattt tatagagaca ttaactaaag
    44221 aaaaacaagc tggagtttca ttaggataat aattaaaata ctaagactct tttttaaggg
    44281 atgttagatg ttataggagg cttattgtat gaatgaaaag tcaagtttta aacaaattga
    44341 agttatttta gcagttattt ttttggtact tacatattac aaggttagat taactccagc
    44401 atttattgtt atatttatac cactatattt aagttatttt gcagtatcta tgattgtaaa
    44461 tcaaatagta attaagctat tggagataag gcaagctagg gaagctacta attacaatat
    44521 aggaaatgga tagaaagaaa aaagaaatat taacactgtc tatggggatt gctttactac
    44581 ctcctttatg ggcagtatta gcaccttata taggaataaa aactggagct gtagcactaa
    44641 tctgtgcagg attatatgta actaatggta ataaacaaaa agatggatta aaaataatgt
    44701 ttggtttttg gtgtggagat ttatgggcag tgttagccat tttaattatg ggatatatga
    44761 atttcaatca aaatctagaa ttatttttaa cacttagtat attaggtttt tttgcagttg
    44821 taattgcatc cttatttgaa aaaataattt tcctaccatc atggctttgt ggttgggcta
    44881 taggtctaac tattatgaca ggtgaaaata ttattaattt acaagatatt tgtattcaaa
    44941 ttgctgtagc tatggcagta ggagtatggt acgtgggtgc aggtgtagat ttgtttacaa
    45001 tatatatttt tagtaaagat aggaaaaagg gaaattgaaa gcatgtgaat atactttgat
    45061 tataggagaa tggctatggg ttttgaattt aaaataatga gaagtttaat atatgtagga
    45121 cttgccaagg aagaatatag acctaagcta atggactggt tatatcgtca ccatattcca
    45181 gatagtatta gcacttttgg accatattgt actaaatatg ccttttatca agcatatcct
    45241 acaccaaatg aaggtgagcg ttttggtgca cgtaagatgc aactaacaga acattattgg
    45301 cttgtagatg aacatatgcc tgagatggca aatagaatta tgacagaata tatgcctatg
    45361 gatgttctac gttggcaagg gtgtatacca gatgtagaaa ataaaagggt tcatgaaaat
    45421 gcagaaagtg gagatgcagg acgtgcagta ggtggagata atggatgtcc accatttata
    45481 tttgcctttg ttccaataaa ctgggaagaa gactttagag gaaaaggacg tactgtacaa
    45541 gatggaccaa actatcgttg gcaatttatg attaagtatc cagatggtat ctctaaagaa
    45601 gaaggagaaa aatggttcta tgatgaggta gtgccatact ttacaaactg ttgctatgtt
    45661 aatcgttttg tcagtagtaa aataatgatt aattatggag caactgcttt tgaccgtgtg
    45721 tcagaactat ggtttgaagg ggaagaagaa tggtataaag ctgtggttga agagacaaaa
    45781 tcgtttatta aaaaaccaga atgggcacaa gaagaggagt tcccatattt aaaaccacaa
    45841 ttcaatatcg catcagtatt cttaggtgat atagcaacta tggatgcata ctcacagtat
    45901 cgtggatata taccaatgag ataaaaatta tagtttgtga taagtagagg aggcatttac
    45961 tcttactggt agaagaagac ttattatcct taaaagtagg tgaatatata gttttatatt
    46021 taaatttata taaaactaag actaataaat atgaaacaat agaattagaa taatttgata
    46081 atatttgtta aaagagcatt tagaatgtgt ttaaagatta agatattcct tttatggtag
    46141 cagatagttg aaataataaa actgttatca tgaaaggaat atctttatgt ataggaaatt
    46201 tttaaaaata aaagttaaat atgtttaaga acatcttaaa gtgaaagaat cattgttaga
    46261 aatatgtaga caatatagta tttataacgt atcatattaa caagtttaca cttggattat
    46321 gaaataaaaa gagtaattcc aaaggttgta aatactatgc tgactttgat acttcaaagt
    46381 ttaggtgatt ttgtaattgt taatagaaat acgtttaatg agcataaaga atttatctaa
    46441 taataggaga atataatgta tacgattgga caagtatcta aatttttagg aatatctaga
    46501 gatacactaa aattttatga ggacaaagga ttagtaaatc ctaagaaaaa tgatgaaaat
    46561 ggatatagaa tatataatca ggttgatatc tatgatattg caacaataaa cttttataga
    46621 gaaattgata tagaaattaa aaagattcaa gaaataagaa aaagtaagag tataaacaat
    46681 ttagaactct tactagaaga gaaggaacaa atcatactaa aagagataga atataaaaag
    46741 cttctcttaa aaaaggttca gttagtcaaa gaagattgtg acaaaatcaa aacatatttt
    46801 ggaaaataca caataaaaga aatgaagcct ttggaagtaa aaggtaaaat aaaacatgtt
    46861 actaactatg aggaatatga agccctaaaa gagaatacaa acaacttaaa aaaagcagtt
    46921 acattaactg atttaagaag agtaataaat tttaatgaag aaggtataat agaagaaaaa
    46981 tttataattg taagggaagt agatgactac aacaaaatca tagaaggtga agttctatct
    47041 catcccaatt gtatttatac tattattgaa gatggcagat ggtcaactgg aggagaaaat
    47101 attgataata aagtggaaga cagattaaga gaaatagcaa tagaaaatga atgtgaatta
    47161 cttggaatag tctatgttaa tatattgctt actacttatg aagaagggat tgagcgtata
    47221 tttttagaga tttatgcacc tataaaataa tataaaagca ttgacctggg acttactcat
    47281 aggtttaaac tcatgatata agcagttata acttatatag caattaaata ttaatttgaa
    47341 atgaggatta cttatgagaa cagagaaatc attaaaaagg cctatagcca gttttattat
    47401 tctaacaaat ataatttttt taccactttt tttattggta gtagtgacta taatgttaaa
    47461 acttccaaca gtgatatctg acattgcgtt gtgtatatca gcgtggtcat ccacttttgc
    47521 atttatgata ttatttaaaa aaatttatcc aggtaagaaa tttttagtac acgtaaaaga
    47581 tagatttaga aataaactta aattttcaac agtatctata ataattagta tacaagtcct
    47641 aatagctgta gtagttatat ttatattagc atctaaaaat catggaataa tgcctaactt
    47701 tacagtttca tcatggggag catttatata tttattttta aaaaatttat ttgcaggatc
    47761 attaggtgag gaattaggtt ggagaggatt tgttcaaaat catcttcaga aaagacattc
    47821 tcctttaaaa gcatccataa tagtaggttt ttggtggggg atgtggcatt taccaatatg
    47881 gtttacaaca ggattcaaag ggcttgattt aataaaatat attatatttt ttatgattgc
    47941 aattatatca atcagtatta taatgacaac tttttataat ctaaataaaa ctttgataat
    48001 acctattgta atacatcaac tatttaattt tttaataggg ataattaatg gagatttaat
    48061 agagcttata aattactatg caatgttata tttaatattt gcaattgcat taatagttat
    48121 aaatccagga aatgtattat ataaaaatat tagagagtaa tatatatctt tatggtaaga
    48181 tataaagaaa ttattagaag aacatccaag gaggaataaa catggtttta ctgattgttg
    48241 atgcacaaaa gttaataaca aacgagaggt tatataaatt taatgaattt gtagtaaatg
    48301 tagaaaattt aattgataca gctagaaaaa ataatataga agtaatatat gtacgtcatg
    48361 atgatggagt tgaaaatgaa ttgactaaag gtaaaaatgg ctttgaaata tacgaaaaat
    48421 ttaaaccatg taatggagaa aaaatatttg ataaaaaagt aaatagtgct tttaaagaaa
    48481 ctggtctatt agaataccta acaaataatg gtgaaaaaga tattattatt gctggtctcc
    48541 aaacagatta ttgtattgat gccactataa aatgtggatt tgaacatgga tttaatataa
    48601 tagttccagc ttattcaaat acaactgttg acaatttttt tatgtcagca gaacaatcat
    48661 ataaatacta caatgaattt atatggaatg aaagatatgc gaagtgtatt tcattagagg
    48721 aaacacttag aaaaatgaaa taaaaataga gtatgaattg tatatgtaca taagttgatt
    48781 tcataaaaaa ggatgttaaa ttccaagtta aaaat
//
LOCUS       DAEMIK010000009        47743 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEMIK010000009 DAEMIK010000000
VERSION     DAEMIK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.009"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..767
                     /locus_tag="KPK87_000450"
     CDS             <1..767
                     /locus_tag="KPK87_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG2536645.1"
                     /translation="MIDKKTQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQN
                     NINISKSGYSSINAKQVPYNLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKF
                     TGDSIDLNVDLDTIMNNKDAKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKK
                     RKISFKNNKNISIINGFLRYPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKF
                     KNKEDVKNYMFNRLKMSEGEIELPDPIIMENDKLMK"
     gene            complement(1080..1859)
                     /gene="hisJ"
                     /locus_tag="KPK87_000451"
     CDS             complement(1080..1859)
                     /gene="hisJ"
                     /locus_tag="KPK87_000451"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG2536646.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2405..2755)
                     /locus_tag="KPK87_000452"
     CDS             complement(2405..2755)
                     /locus_tag="KPK87_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG2536647.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2771..3187)
                     /locus_tag="KPK87_000453"
     CDS             complement(2771..3187)
                     /locus_tag="KPK87_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG2536648.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAISDSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3206..3397)
                     /locus_tag="KPK87_000454"
     CDS             complement(3206..3397)
                     /locus_tag="KPK87_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG2536649.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3548..4363)
                     /locus_tag="KPK87_000455"
     CDS             complement(3548..4363)
                     /locus_tag="KPK87_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG2536650.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4410..5348)
                     /locus_tag="KPK87_000456"
     CDS             complement(4410..5348)
                     /locus_tag="KPK87_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2536651.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVNKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5345..6217)
                     /locus_tag="KPK87_000457"
     CDS             complement(5345..6217)
                     /locus_tag="KPK87_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2536652.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6242..8170)
                     /locus_tag="KPK87_000458"
     CDS             complement(6242..8170)
                     /locus_tag="KPK87_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG2536653.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8282..8659)
                     /gene="gcvH"
                     /locus_tag="KPK87_000459"
     CDS             complement(8282..8659)
                     /gene="gcvH"
                     /locus_tag="KPK87_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG2536654.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8746..10872)
                     /gene="cdhC"
                     /locus_tag="KPK87_000460"
     CDS             complement(8746..10872)
                     /gene="cdhC"
                     /locus_tag="KPK87_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG2536655.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10929..11735)
                     /locus_tag="KPK87_000461"
     CDS             complement(10929..11735)
                     /locus_tag="KPK87_000461"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG2536656.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11785..13152)
                     /locus_tag="KPK87_000462"
     CDS             complement(11785..13152)
                     /locus_tag="KPK87_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG2536657.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13183..14127)
                     /locus_tag="KPK87_000463"
     CDS             complement(13183..14127)
                     /locus_tag="KPK87_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG2536658.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14147..14917)
                     /locus_tag="KPK87_000464"
     CDS             complement(14147..14917)
                     /locus_tag="KPK87_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2536659.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14973..16358)
                     /gene="lpdA"
                     /locus_tag="KPK87_000465"
     CDS             complement(14973..16358)
                     /gene="lpdA"
                     /locus_tag="KPK87_000465"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG2536660.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16402..17283)
                     /locus_tag="KPK87_000466"
     CDS             complement(16402..17283)
                     /locus_tag="KPK87_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG2536661.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRNMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17319..17993)
                     /locus_tag="KPK87_000467"
     CDS             complement(17319..17993)
                     /locus_tag="KPK87_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG2536662.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18030..18902)
                     /locus_tag="KPK87_000468"
     CDS             complement(18030..18902)
                     /locus_tag="KPK87_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG2536663.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18994..19626)
                     /locus_tag="KPK87_000469"
     CDS             complement(18994..19626)
                     /locus_tag="KPK87_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG2536664.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19731..21407)
                     /locus_tag="KPK87_000470"
     CDS             complement(19731..21407)
                     /locus_tag="KPK87_000470"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG2536665.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21656..22435)
                     /locus_tag="KPK87_000471"
     CDS             complement(21656..22435)
                     /locus_tag="KPK87_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2536666.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22745..24664)
                     /gene="cooS"
                     /locus_tag="KPK87_000472"
     CDS             complement(22745..24664)
                     /gene="cooS"
                     /locus_tag="KPK87_000472"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG2536667.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25271..26182)
                     /locus_tag="KPK87_000473"
     CDS             complement(25271..26182)
                     /locus_tag="KPK87_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG2536668.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26298..26876)
                     /locus_tag="KPK87_000474"
     CDS             complement(26298..26876)
                     /locus_tag="KPK87_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2536669.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            27102..28025
                     /locus_tag="KPK87_000475"
     CDS             27102..28025
                     /locus_tag="KPK87_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2536670.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(28197..29339)
                     /locus_tag="KPK87_000476"
     CDS             complement(28197..29339)
                     /locus_tag="KPK87_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2536671.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALKLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(29357..31111)
                     /locus_tag="KPK87_000477"
     CDS             complement(29357..31111)
                     /locus_tag="KPK87_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG2536672.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            31247..31453
                     /locus_tag="KPK87_000478"
     CDS             31247..31453
                     /locus_tag="KPK87_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536673.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(31458..32381)
                     /locus_tag="KPK87_000479"
     CDS             complement(31458..32381)
                     /locus_tag="KPK87_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536674.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(32635..34335)
                     /locus_tag="KPK87_000480"
     CDS             complement(32635..34335)
                     /locus_tag="KPK87_000480"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG2536675.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(34747..35160)
                     /locus_tag="KPK87_000481"
     CDS             complement(34747..35160)
                     /locus_tag="KPK87_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG2536676.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(35226..37604)
                     /locus_tag="KPK87_000482"
     CDS             complement(35226..37604)
                     /locus_tag="KPK87_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG2536677.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            37923..39374
                     /locus_tag="KPK87_000483"
     CDS             37923..39374
                     /locus_tag="KPK87_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG2536678.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(39578..42091)
                     /locus_tag="KPK87_000484"
     CDS             complement(39578..42091)
                     /locus_tag="KPK87_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2536679.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEDCIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNEESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(42314..43135)
                     /locus_tag="KPK87_000485"
     CDS             complement(42314..43135)
                     /locus_tag="KPK87_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2536680.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(43653..44129)
                     /locus_tag="KPK87_000486"
     CDS             complement(43653..44129)
                     /locus_tag="KPK87_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG2536681.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIIYDNGKLKDYNVSFLD"
     gene            complement(44164..44742)
                     /locus_tag="KPK87_000487"
     CDS             complement(44164..44742)
                     /locus_tag="KPK87_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2536682.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGNEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(44846..47446)
                     /locus_tag="KPK87_000488"
     CDS             complement(44846..47446)
                     /locus_tag="KPK87_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG2536683.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFLEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        14592 a         9144 c         5366 g        18641 t
ORIGIN      
        1 atatgataga taaaaaaact caaaatagtg taaaggaaat cctaaaagaa aataaaatta
       61 atgaaaaaga catagataca tttattaaag cagtcaataa ttataataaa ttgcaagtta
      121 aaatacttca aaataatata aatatatcta aatctggata ttcttcaatc aatgcaaaac
      181 aagttccata taacctagaa aaattgcaag ataattgggt gaaaaaattc cctgattata
      241 tggatgtaaa ttgtagaata actgctttta gactttttaa agattttata aattcaaata
      301 agaaatttac tggagactct attgatttga atgtcgactt ggacactata atgaataata
      361 aagatgctaa atttagcacc aaagatgtag aaaaatttat caacttcttt tctgcaatac
      421 cagctaaaga tacagatgat acaatcaaaa ttgcagaaca aataaaaaat gaatggaaaa
      481 aaagaaaaat atcttttaaa aataataaaa atatatctat tataaatgga tttcttcgtt
      541 atccagaaac taaaaatgta tttataggtc atacaggcat atgtattaaa actaaaaatg
      601 gcattctatt tttagaaaaa tatggtgtta catcacctta tcaagttact aaatttaaaa
      661 ataaagaaga tgtaaaaaat tatatgttta atagattgaa gatgtcagaa ggtgaaattg
      721 aattacctga ccctataata atggaaaacg ataaacttat gaaataataa ctttaaacgc
      781 aaaatagtat ttataattac taagtcaata taatagagaa ggtctataga tttgtgtaaa
      841 tacaagctta tctacaatct tatataagaa tgtttatata aataagatag attaattcta
      901 agtcctatta ttctattgaa ttttattatt attttattta gattctattg ttattttact
      961 tagaacttat tattattttg tttaagttct attactattt tcaatcatat aaatttacaa
     1021 aagactgtac ataaatttat tttatacaaa cttatctaca gtcttttgtg tgaatatatc
     1081 taaataatgt cgtatttatt tatttcctct gcacagtgag aatcagaacc taaaactaat
     1141 ggaatatcgt atttttttac caactctaat aagtatccat ctatataagg ctctttgcag
     1201 tactcttttc tatttccaga aacattataa tcaagttcat aacctttttc tttaattaac
     1261 tttattaact cttcaagaat tgtatttcct ttataatcgt atggaaatac ctgattaaac
     1321 tttctaacta aatttaaatg acctattctt tttggtttat gtatacctaa atctgaattt
     1381 acagctttta tcaatgtttc ataatactta ttataaacag cttcaatact tcctaatttg
     1441 tcgattaaat tttgaaactc ttctacactg taatctacac aataataatc atcatcaatt
     1501 ttaattatat gtactgataa aagagagtct tctaattcct ttccatattt atttaaattc
     1561 tcctttatcc cttcttcgta tccttctata aaatccactt ccgacccaac atttatcttg
     1621 attttaccct catacttttc tttcaacttc ttgacatcat taaaatactt tggtaaatct
     1681 tcaatactca tacaactatc attttcagga gcagggtctc taaatccatt tgcatacgga
     1741 aaatgttctg taaatgtcat ttctgttatt ccaacatcca ttgctctttg tacatatttt
     1801 tcaaaatcat ccttacttcc atgaggacaa taaggtgaat gtatatgacc atctttcata
     1861 aataattcac tcctttttta aagaaaaaat ctaatataaa tgcataaatt ttttaacttc
     1921 aaaataatat ttttaatcat gctaattttt attatatcta atattagagt aattttaaac
     1981 tgccttttaa gtatcttatc attagtacta ttttcaactt aacattaata ttatttttta
     2041 acttaacatt aatactatct ttaaatagta atactcaata tataattact tagcatcaaa
     2101 taactcaaat tgcttgatga ggcttttgca gtatttaaag aatatataat tatctaacat
     2161 taaataatcc aaccttgtaa taaaatttac tcattaaagt atattacaga aaatacctca
     2221 aaataaactt tttagttcat gatatactct tttttgcatg aaattagata tatttttatc
     2281 atttcaacaa tgaaagggag gcttatatcc atctttagac aaccccttag ataaaatttt
     2341 taataattaa acatcacaaa atataaaaat cgcttatatc aaacgtataa gcgattaatt
     2401 atatttacat atgtttttta gcctctaaca acattttatt agcttcttcc atatactcta
     2461 tagccttttc aatactcttt gctgtttctt cttttccaat agctctagcc ttttcaaccc
     2521 actctctaaa tccttcttca tgagtttcat tgtgatttat ccaatgtact aaaagtattt
     2581 ttaaagtttt ttcatcctta gactctgatg attcctcatg actatgttcg tggtcgtggt
     2641 cgtgagcatg gtcatgtgga tgttcatgac atatatctcc atgagtatgt tcgtggcagt
     2701 ggtcatggtc atgatgtcca tgatgatatc ttacaatctc tttatcaaaa atcattagtt
     2761 attccccctg ttattttata ttaaactctt tttttataag gtttacagaa tcttttacaa
     2821 gtagtgctaa tggtttacct tctaatccaa ctaactccac attttctggc ataataggta
     2881 aaagtatctt aagtgcctca ctgtctgaaa tagcttcact tatattacaa gtaacttctc
     2941 ccatcataga attaggtatt attacagaaa taggtgcaac tattatattt gcttttttag
     3001 ctgatactac tatagcattt tcccctgtag ctcctttatt agcatgagct ttcatcatag
     3061 aactagttgc tatagaatta gtccctagtg cataaatttc tacatatgta ggtaactctt
     3121 ctctcaaaga agaaactatc tgggctccaa tacctccacc cattccatct atcactgcta
     3181 ttatcatatg aaattcccct attaattaat tttcttgtat tattatctta tggtccatca
     3241 atcttatttc tttaattatc ccatctatta tcttttgctc tcctaataaa tccgttaaat
     3301 aaatctttcc atcacaaggg tctatattaa ccacattttc cataaccttt tctaactcat
     3361 tattactttt acaaatgaaa gctgatgatt cacacatata aatctcctcc taatatgatt
     3421 tttattaaaa atcaaaaaaa gattccctta ggtatttcaa tacaccaaag aaaccttcat
     3481 gatttcagtt tttgtatatt attatacctt aagtatatca ctttaattta tgtaaagtca
     3541 attaaagtta acgcttttta ttatactctt cattcttgta tttttcatat agttctttaa
     3601 ctaattgctt ctcataatca gtcaaatcgc cctccacaaa ctcaacatta aattgttcag
     3661 ctaatccctt tactatgttt ttctgtagaa tatctatgtc tatttttctg ccaatttcat
     3721 tttctattcc acttgctttt ttagctgtaa atttcatagc tctttctttc aattctggtg
     3781 tcttagtttt tattaattta aacaattttt ctacatcaaa atccagtatt attgaaccat
     3841 gttgtaataa aacaccatct tttctgcttt gagcacttcc aattaccttc ttattattaa
     3901 tagtaacttc atatgatgca tgtgcattaa aacatgcagc tgataaattt tccctactta
     3961 ttctctcgcc tctatttaaa ttatctgttt ctataccact tagattcaac cctttaacta
     4021 gaccttcact tatatatcta taagataggt tgatgctctt atcgattaaa ggattatctt
     4081 ctcctataat tatactataa gttagctcat tatcatgtaa tacagctctt ccacctgtaa
     4141 ttcttctagt atagtctata ttcatacatc tacacttatc taaatctatc tcatcttcca
     4201 atttctgaaa atatcctata ctcaaacaag ctggttccca tgtataaaat ctaagagttg
     4261 gcttattatg tcccttctta tatgcagtaa atatagcttc gtcaattgcc atattcattg
     4321 ctccttcata tgatttatta tgtataactc tccactgatt catgattatt acgcccccat
     4381 ttttagtata aatatatcct agaaatcctc tatagattta tttctcttat tgctaaatct
     4441 atctcacttt gatttaaagg tcttgaatag ttgtatatag tacttcctgg tctttcatta
     4501 tagtatggtc tattacaatc cttacaacct cttatttcaa aaggatagcc tttgtttata
     4561 tcttttaaaa tatcattgtc aagctttata ctatttaagt atccattttt aaacttaaaa
     4621 tactcttttg gataaccttt atttatcata taagacatta actgtaccct tctataactt
     4681 tctatgcttg gttgacttat cttttccatt ttagttcctc taactggagt aaatgcaaat
     4741 aaactaatcg ttacatcatt ttcctttaaa taagtatata aattgtatat atcttcatga
     4801 ctttcaccca taccaactat tatatgtgta cttattttat tgggatatga ttttgacatt
     4861 tcagttataa attttaactt ttcatcatag ttatttcctt ttattttttc atatagttct
     4921 ttatttgctg catctattgc tattcctatt ttatttactc ctgctgaaaa aaacttcttt
     4981 atctgttcat cattttctaa ttttgcagat agtgaaattg gcatgtctat cttaccactt
     5041 atatagttta taaagtctaa aacagagtta tgtgcttctt cacttgccat tgattgaata
     5101 catattctct ttatattttt tcctttataa gcttttaaag catctaatat ttcttctttt
     5161 gaaaactctg gccaaattac tcttgataat ttatcttttc tagtactact ttctattgat
     5221 tgagaacaaa aactgcattt atttatgcat ttttctccaa tcattatata tgctgtggta
     5281 ggaggtatat cgcttttttt gtttaatatc ccaagttcta ttgctgtccc aactgatagt
     5341 ctaatcataa aacgcaacac tccttacact catttattgt catattcatt tctatagcct
     5401 tattctttgc agaccttgat ggtaatacta ttctattgac tccacacatt atagataatt
     5461 ggtctagctc ttttctatac actcctcttg gtctcataca acctagatta atttcagtat
     5521 ctggtaaatt aattctagct tcacataaaa tatctgctac cccttctaac tcaggaggtt
     5581 ctacattttc atactcagtc ccttttgttg gaataaggac tataaatgta agcttattag
     5641 gaggattttt ttgtaggtat tctataattt catattcacc ctttatttgt cctcctttta
     5701 aaccaataca tatatgtggt gctacttctg tatgttcttg tattgtatta tagacttcaa
     5761 tataatcttc tttactcttt ttcatcttat aaacttctct tattgtctca tcatcaaaca
     5821 ctaaatcaaa agaaactata tctaaatact tacacaactc tacaatgcta tctttatcca
     5881 taagacctaa atgagcattc aatctatatc cttgctcttt taagttttta atggtattaa
     5941 tatgagtatt tatagggaca tctccctcat aactacaacc gccactcaat agaaagctag
     6001 ttatattcct tgattgtact ttttcctcat attcttctat tggcaccata ttgtttaagt
     6061 agtgaccgtt gcaatgggca catttcaaac tacattgatt gcttgtagta ctaagagcta
     6121 gtgtctggtt aggataagca aactctattt tatttccaaa attctttaat ttaatatcaa
     6181 atgctatttt cattttttca gatagattct tattcatagt ttattccttc ttaagtacat
     6241 tttactcttc tagtaatttc tttgctgttg ggaactgctc tatattcgta tgtggtaaga
     6301 aacatgcaga aataaattca tccatataac ttggatatac actaagttca acataagtta
     6361 tttgattacc tatttcttca agtttatttt ttgcatcttt acttataaga gctaagtaag
     6421 aaccaacaag tgaactattc cctatataag taaatttttc tctatcaata tcaggaagta
     6481 accctataat tattgagttt tctatgtcta ggttgtttcc tataccacct gctatataaa
     6541 ccctatctaa tatactaaaa tccataccta aactatctac aagagtgtat gcgcctgaat
     6601 atatagctcc tttagctctt atgaaattat ctatatcaac ttcattgact actatatcat
     6661 tttctaaatc aaattcttct ttaaatgcta atacatactc tcctatctca tgctcattaa
     6721 atctaactct cttattatct aaatccctgt atattttacc tcttctatct attacacctt
     6781 tggtaatcat ttgacagatt aaatcaatta taccagaacc acatatacca actggtgcac
     6841 attcatcaat aatctttaat gttggctcta aagtatcttt atcaataatt actttctcta
     6901 ttgctcctgc tgatgctctc attccacagc ttatccctcc accttcaaaa gcaggtcctg
     6961 ctgaacatgc acagctcatc atataatctt tatttccaaa tacaatctct ccattagttc
     7021 ctaaatctat aaataaaaca ttttcttcac ttgaccatat ccctgctgat aatactcctg
     7081 ctgttatatc tcctcctaca taacttgcaa cagatggagc taagtacaca tatgcactgt
     7141 catttacaaa aagaccaaca ttttcaccca taagttttgg agattttaaa aatggtggta
     7201 tgtaaggttc ttgtcttaag aagtctgtat acacacccaa gaataaactt gtcatagttg
     7261 tattcccagc agccactaat gtaactacat cttccttatc tattccattt ctttcatata
     7321 ttgtttttaa tagtggattt atagtttcct caactatagc tttatgaagt gtctctaatc
     7381 catttttctt agttgaatat ataattctat ttataacatc tgctccatat tttatctgtg
     7441 catttccaga agatgcttta tctactacct cttttgagta taggtctact aaacaaacaa
     7501 ctactgaagt tgtaccaata tctattgcaa ctccgtataa ggaattttct ttatttcctt
     7561 gttctatatt tatgattgta agtttctttt gtttttgaac atatgttatt gtaaccttaa
     7621 aatcactctt tctaaataca gttggcattt ttctaagtat atccaatcta aaatctattt
     7681 cattatatcc taggttattt ctaacatatc tttccaatct atctacatca cttatattat
     7741 catctaaatt tggttcttcc atttctatgt atttcttttt tatatttgtt ttaaattgaa
     7801 ggttatgttc ttcaattatt tttttagctc tttcaaaaat ttccctatcc tcttttttat
     7861 tactaccttc tatcttcata ccatgcatag aggatgatac tttagaagga acttcaattt
     7921 ctatatctga tattaccttt gtacaacaag ctagtatata accttgctcc cactcatcat
     7981 ctgttatatg tcttgttttt tctgtatcaa cttttccatt caataatttt actttacact
     8041 tcccacaaga cacgtttcca ttacaaggag catctatgaa tatatctgca tttcttgcaa
     8101 cctctagtaa tatatccccc tcattacaat aaacctcttt attgtttggt gtaaaactta
     8161 cttttatcat gttatccctc ccaatttgtt ctatatatga taaaaggaaa gtatagctaa
     8221 atttgcgtgc actcaaattt tactatactt tccccaatta taataatatc ttaatcttta
     8281 attattctaa tccagcctcg tattctttat cacttaataa cgtatcaagt tcagccacat
     8341 cagctagttt tacttttaat atccagtttt catatgggtc ttcatttata cattctggct
     8401 catcttctaa tttttcatta acttccaata cctcaccatt aacaggagat attaaatcag
     8461 aagctacttt agaagactca actactccaa aatctactcc cttagttaca gtatcttcta
     8521 cttctggtgt ttcaacaaac aatatttctc ctaattgatc ttgagcatag tctgttatac
     8581 ctatatatac aacatctcca tcaattactt ttacccactc atgatcttta gagtatttta
     8641 attctggtaa taatttcatt tcgatagacc cccaatatta tattattgat taaattactt
     8701 aaatcatatt aatattatat catattattt taaattttcc atcaattaca ttacactttc
     8761 catagctaat gctggatgac ctttttcttc taagaaagct aatactcctt cagaatcgtc
     8821 agctatagtt tcatcacaaa ccatatcaca gaagttttct attccagtca tttctttaac
     8881 tgtagcattt aatttatcag ctacataatc ttttaattct tttggcatcc aaactattct
     8941 ttctggtcca ccttctgcat aagcgaattt tttagaagat atgaaatgtc ttccgtgtcc
     9001 cataaatcct ggagtttgaa ctcctccacc tgtcatagaa gcaagttctc cgaaagtcat
     9061 accaactgga gtaacacttg caaattctct gttaactaca acaaatccat ttgcttctgg
     9121 cattatacca cagatacatt cgaagcatcc acaagaagtc attgggtctt ctagtataga
     9181 gtataatgta acagattcaa ctgcaccttg agatatttgg tttacagttt catttactga
     9241 attccataca cctgttctat catctaagca ctcgcctttt tctattggtt gacaaggacc
     9301 tgttgggtct agttctttag tagccttagc atctaaccag ctaactgctc cacaaagacc
     9361 aagtctttca ggagtaacaa cacaaacgtg agctggtgca aatgattgac ataagttaca
     9421 agaatagaaa gtgtcaacac tttcatcaac taatgaagct aatctttcat ctctagcatt
     9481 gtacttagct attgcttcac cttttaattc agaaactttt tcagcatctg taattatagt
     9541 tatttcacac ttatcaacta ctgattcaaa ttcatctaac atcttagcat ataaaacttc
     9601 tcctatatgc tcaagtctaa atcctttatc aaatgcatct ttagagattc ttacccaagc
     9661 catatctctt tgtccaacgt gcattacacc ttctatatag tttaagaagt agtggaaacg
     9721 tctttcaaga actggctcga aatcttcttg catattttta ccagctattt taactattac
     9781 agcaagtggt aatcttaata caccatctgc atcaacttca tctatatttg gtcctattat
     9841 ttctatctta tggtcttcaa cttctgttgc ttctttctta acaacaagct cccaagcttc
     9901 agttctgttt ccaccaaact cagcaaacat atcgctcttt ctaattctct caccttcaaa
     9961 tgctgctgca aatgaaactg ggataggtat ttcagtaact tttattttta ttcctctagc
    10021 ttctaatgaa gtagctacta tcttatcgta atctttttga gttaataggt tcattggaac
    10081 ttcaagaact gtttggtcag ttataactgg gaatcctaaa gctattgctc cagcacctgc
    10141 agatacaact aattcactta atggtccaaa tgcattaaca aatgcaggaa ctctattagc
    10201 tgtgtactct aataatccgt ttaaatcacc aggagttaat ccaccgaata ttaatgctgc
    10261 tcttactgca acagatacaa cgtgtataac agaagttaca tcatatccta gaggtataac
    10321 tcttagctca agacccattt ttactccagc ttctatagct tggtctatga ctttaccaac
    10381 taagaaagtt agtaaacctt tagattggta atcttttata acttttgctg ctgattctgc
    10441 atctggacac tcaccaagaa caactgcaac accaggtata tctcctgtaa ctaatggtac
    10501 accaagtgat ctgattatag ggtcagttat atgacctgca catggctcac tatatggagc
    10561 atccatagtt gaatatttta atgcttcaat tacttctgct gctaaagctg tagctaaacc
    10621 agcattaaaa gcatgttcta gtaaatgagt tctatttatt aaagatttta ctacttctaa
    10681 agcaccttct aagtccccta aagtgttcat cttttgtcct gttgcagcat atatacaagg
    10741 taatgaatat gctgtatcag ggaaagcaac tttatgctct tttccattct tttctatagc
    10801 ttcagctaac atagcttgag ccgcacctaa agcttgttct gaccctgtaa agattatatt
    10861 atatagattc attctatttc cccctttaat atttacacct attttttatt aactataccc
    10921 taaaaaccct agaaagcaaa tccaccttca tttaactcta agaaagagtc tgcatataaa
    10981 gatgccccgt tgattatgtc acaagatttt acagttttca ttaattctgg gtcacatgga
    11041 ttcattatag ctgaactaaa tccattagcc attgccattg ctaagaatgc actatctaaa
    11101 acaggtctaa catgcttagg acatccatta gatacgttag ataatcctcc tgtagttaaa
    11161 agtcccatct cagtcatcat ttttatagct tctaaaactt ctacttgttt ttcttgcata
    11221 ccttttataa ctaagcataa tgggtcaaat agtatatcag ttggctccat tcctaacatt
    11281 aagccttttt ctaatatttc ttggcagtaa gccatacgct catcattatc tcttgggata
    11341 ccttcttttg cacataaacc tataaccatt gcttcgtatt cagctgctat atctattaaa
    11401 tcaaatcttg agccagcatc agcagagttt attattggtt ttgcattagt tctatcataa
    11461 actttaagtc cagcttcgat agctttttta ttagctgtat ctaatgctat aggaacattg
    11521 ttgaactcag attgaagtaa tttgattccc catgtcatta tttcttcgcc atctctttcg
    11581 gcaggtccta tattaaaatc tatatagtgt gctcctgctt ctaattgctc ttttgctctt
    11641 tttaagattg gagctgggtc tctttctgct aatgcttttc ttattgaagg tgatatacaa
    11701 tgtattctct cacctataat cataaatttt tccatttata atgtccccct ttaaatttaa
    11761 tattaatagt tagttaatat tttattagtt tgtttggcat gctttttctt ggtatccttt
    11821 taagaactta ggtaattcca tagattcctc tggtcctata actacattcc atcctggtaa
    11881 gttatcttct atatcacctt taagtactgc aacttttcct ggaatgataa ggtctttaca
    11941 attagttact tcttctacct tactttcttt aatgaaagca gagattgagt ttccaccaaa
    12001 tttaccagca gcccaagatg taagaactga atatccacct gcatctggta ttactaacca
    12061 tactggaact tttgatcttt ctatatcacc agcaactatg aagtaagtta atgcaaagtc
    12121 aacagtaact aatactggag aattttcatc tgggttgttg attggataaa tctttggttc
    12181 aactctcatt ggtctttgtg ggtcagtgta tatgttttgt cttagtgcaa ataatggtaa
    12241 tgccttagca taactaatat catctattac tattatagaa ccgtatttta tagtgaatat
    12301 tgatgataaa gcaacttcca tcatagggtt tgaattagat aatctatttg caaatactat
    12361 tgaaggatat ccaaatgttc tatcttgctc ttttaaagct gttcttctaa cttgtatagc
    12421 attagtgtaa gtatctttta tgttttctcc tgttacatct aatactaatt ctttatatcc
    12481 agcagcttga attaattcaa ctgtttcata taattcttct aagctaccag cttttacacc
    12541 taatggtaaa cttgctcctt taactacttc tatcatatct ttgtagttct ctttagttgc
    12601 tccatatacc attggtctag catcttttaa tacttcaaca gcttctttaa ctacttgtgc
    12661 atcatcacaa gctaaaatat aagctacttc taatccactg ccttttattt tatctattaa
    12721 ttttaaatac gcatctttat ctccacaata ctctaaaaca gccatttcaa ctttcatttg
    12781 ttcaccaatt cttacatagt caactttttt catgttagct attttagcat ctactgcttc
    12841 atcactcata caagtacaga atgaaactgc atatctattt ctacttacta atgttttttc
    12901 atgtctaaat aatactgttt ctccacctag ttcatactca gatgcaccag caccaacttt
    12961 taaagctttc attaaaggtg cagtagcctc tgataatttt gctatagcgt catcagacat
    13021 atgtgggcat ttacctactt ctacagctcc tgaagcaacc ttcatagaga aagccataca
    13081 agttgggaat ccacaatcct tacagttttt ctttggtgtt aatttaaata tatctaaagc
    13141 ttttaatgcc attatttttt ccccctaacc ttaacaattt aattaagcta atgcgttaac
    13201 caattctttt atagtttcta ttgattttgg atgacgaagt ataactgcgt ttgaaccacc
    13261 tactaaaaca cttgctgcag ttgaaacttc cattgctata gttctttctt ctggacaacc
    13321 ccaatctggc tcatcttcta ttggagctat agcttcttta acatgaccta cttcaaaagc
    13381 tactggtgtt atgataggca tttgtaatgt cttatcattt tgaccaaatg ctgcaagtct
    13441 aattctatcc atagtagatg caacgtactc atatccataa ccaactgctg aacatccaac
    13501 attcataact atattttcac ctttaacgcc tagttgagtt aacaatacgt ttaattgttt
    13561 agctaggttt atatcaacag aagattcagc tcctacttta tgtgcataag ccataccagc
    13621 tgatgctcct actcctttgt agttatcttc tactgctgac ataaataagc agttatgtcc
    13681 atccaatgtt tgagctaatt tttcaaataa tttaccatct ttttcatgat ttcctgaacc
    13741 agctacaact aaaggtaatt ttattgcttc aattactgcc ttagctacat cagcacattc
    13801 atcaactgac ttatctaatc catttgggtc agaaccatca aattttaaac aaataaaatc
    13861 tgcttgtgta ttagcttcaa catatttagc ccactctact ggacaattag ctacatcttt
    13921 gtataattcc ttatatgaat cagtccagct ttcaggataa acgtctgata tttgtatccc
    13981 tatttttgga gaattaccta cttctccatc aaaactataa aaaggtaata cattttcccc
    14041 acctaattta attgcttttt cccctattcc tacttcaact tctgatattt ttccagaata
    14101 tttttgagta gacattttaa atgccatatt tttttccccc ttaagcctat tttaattcta
    14161 atttagcaaa aatttctttc atagccactt tagattttga atcttcaggt aaattaacta
    14221 aaggaatacc tgtagaatca tattcatata tcaattcatc cataggtaca acaccgatta
    14281 gctctaggtt atgcttttca atttcttctt taacaccatc attcataacc ccatttggaa
    14341 ctttatttac aatcaaaagt attcttccaa cacttaactt tagttcctca gctaaatctc
    14401 ttattctagc aacagcttgt atgctacgtc ttgagcaatc actaaccaat aaaagtgtat
    14461 caacatgtct agtgactttt ctacttagat gttccatacc agcttcgttg tccatgacta
    14521 agtatttgta atgaccagat attttgttta cttgttctct aagtatccca tttacaaaac
    14581 agtaacatcc ttcaccttct gacctaccca ttactaataa gtcatatcct tcgccttctt
    14641 caattataga atttaatcta aattgaagat attgtgcctt tgtcatacca ccagggaatg
    14701 cattgcctaa cttttctctt tgatttactt cttctcttat ttctcctatt gttgcttcaa
    14761 cttcgatacc taatacttca tttatgttag cattggcatc agcatctaca actaatactg
    14821 gtcccttttt gtccttaacc aaataatcaa tcaaaagccc tgtgagactt gttttaccag
    14881 ttccaccttt tcctgcaaca gctatattgt atcccatttt aagacttccc ccttaaatct
    14941 agccacagag ccttatgtac tctgtctgtc tattataatt taggtacaga atgaacacat
    15001 tctccatgta catcatgaag tgcttccatt agcccttcag ataaactcgg atgtggatgt
    15061 attgcatccc ctacttgttc tactgttaat cctagatgaa cagcaagtga caactctgtc
    15121 aataaatctg tagcatgagg tcctactact gctgcaccta ttatcttatc agtttctttg
    15181 tcagctataa cttttacaaa tccttggaaa tgtcctatag cttgtgcttt tccaagccct
    15241 ctaaaatcaa attgacctac attgtattct acaccttcag cttcaagttg cttctcagtc
    15301 ttaccaacac cagcaacttc aggttcagta taaacacatc ttggaattgc tctataatct
    15361 actaccttag tcttacctaa agcattttca actgctacga taccttcttt agaagcaaca
    15421 tgtgctagga aaggagtatc tattatatcg cctattgcgt atataccttc tacattagtt
    15481 tcaagatgtt cattaactac tacttttcct ctttccattt caattcctat atcttctact
    15541 ccagaattgt caaggttagg tcttctacca acacatacta atgcatattg tgcttctata
    15601 acctttccat tagaaagagt tgcaacagcc ttaccatcaa ctacttcaca agtttgtact
    15661 ccaatacctg taataacctt aattttatct ttcttaaatt gtctaagtag ttgtttagct
    15721 acatctttgt cttcatttaa tagtatttgg tcaaccatct caactatagt tacttctgtt
    15781 cctaaggctc taaagaactg ccctatctca catcctataa ctccaccacc tactattaac
    15841 atagactcag gtatctcttc aagtccaaga acttcatcac tagttattac aacttttcca
    15901 tcatatggga acattcttgg tacaactgga acagaaccat ttgctagtat tatcttatct
    15961 gcttttatag tttctactgt tccatcatct ttagttactt ctatagtgtt tttatcaatt
    16021 aattttccaa aaccattaac taagtttact cctctttttt caaataagaa ctctatacca
    16081 cttattaatt ggtttacaac tttatttttt ctttccatta tggcagtgaa gtttggctta
    16141 actgtaccat ctatttctat accaaaatcc tttgcttctt ttacagtatt caatactcct
    16201 gaagaagcaa gaagtgcttt agttggtata catcctgcat ttaagcaagt tcctccaact
    16261 cttctttttt caacaactgt tacatctgca ccaagcatag aagcttttat agctgctaca
    16321 tatcctcctg gtccaccacc tactactact atcttcatct ttaattcccc caaacctaat
    16381 ataatctaca atcttgcaat actataaacc tgcggcatcc agtatagcag gtatattctc
    16441 ttctgcccca attgccatta tatgaacacc atcacataaa tcttcttctt ttaattgttt
    16501 gataagttcc cctgccattt ttattccttc acttacccag ttttcttttc cagctgctct
    16561 taatctttcg atttgttcat ctggaacaaa tattcctggt acatttgcag tcataaattt
    16621 agccattcct ggtgatttta aaggtactat tcctgctaat accttgcaat ccatattcct
    16681 tgttaattct ctaaactttc tcattgtatt tatgtcataa agtgcttgtg tttggaaaaa
    16741 tttagcccct gcttttattt tctttttcat ttttaataat tgaacttcta ttggagaata
    16801 ttctggagtt actgatgctc ctagataaaa atctggagaa ccttttaact tattaccagc
    16861 catgtccgta cctgccatta aagtctcagc agtttgaagt atccccacag aatctaaatc
    16921 aaacactccc ttagcttgtg gatggtctcc tacgctagta tggtctcctg ttagtgccaa
    16981 cagattgttt atcccaaata cacctgctga caacatttct ccttgtatag ctattctatt
    17041 tctatctctc cctgttattt gaataactgg ctctaatcca gcatctttta ataatttaca
    17101 tgtagcaaga gaagttgctc tcataactgc cgattgaaag tctgttacat ttgctgcatg
    17161 aactcttcca actaacggct ttgcacattc tattaaatgt gaaaggtcag tcccttttgg
    17221 aggagccatt tcagtagtaa ctgcaaattt tccactttct aatgtttctc ttaataagct
    17281 catctctaat ccccctttta accttctaaa aaaaactttt atgaatttgc tgctgcttct
    17341 ttctttttct ttgcacttaa acttcttgga ttattttgct tagaatagtc ttttggtggt
    17401 cttatttctg ctaagttatc taattgtcct atagcttcta gtctttcata tatctttatc
    17461 caagcacaat cattctctga gtttacttca cacttaccat ttttagctcc accacaagcc
    17521 ccattcataa gacctttagc acacatagtt actggacata tcccacctgt ccaaccaagt
    17581 tcacagtctc cacaagcctt acaggcttct tcatattctc caactctttg tacttcccct
    17641 ataaacaaag tattgttggc tggataaact ggcttatctt ttaaattctt aactattgtc
    17701 tgtgtaccat ctccacatgc taaagataaa actgcatcag cttccttagt ctcttctttt
    17761 agagccttta aatccttttt agactttaaa aggttacaag cagggtcaag cattacatat
    17821 cctaagattt ttttgccttc accttcaaga gtttccttca tttttaaaac ttcttcttcc
    17881 ccaccacttt tacaagttgc tgcacattga ttacaaccaa ctaaaaccag cttgtcaaaa
    17941 tcttttaagt accctagcac ttcttctaat ggtttatttt cagaaattat catatctact
    18001 tcccccctaa accttccaca tatatatatc tatttattgt tttgtaattt acaagcctta
    18061 actacatgtt ttgcaagtat agatgttgta actgaaccaa cccccgctgg aactggagtt
    18121 atcattgata ctttgtctaa aactgcattt gtatcaacat ctccacataa atttccttct
    18181 tcatcaacat ttataccaac gtctataact accgctccat ctttaacaaa actttcatca
    18241 accatcttag ctctaccaat agctgctatt agcacgtctg cctctgctgc tacacctgat
    18301 agatttttag tttttgaatg acatattgta acagttgcgt gttcacttaa taaaagcatt
    18361 gatactggct ttccaactac cattgatctt ccaagaactg ttaccttact tccttttaaa
    18421 tcaacattat agtgttttaa tatctcaact actgctgtag gtgtacatgg tggaaatcca
    18481 cttttatccc cttccattac cttagcagag tttattggac taaaacaatc tacgtctttt
    18541 tccggtgcaa taacatattt tataacttct tcatttaagt gttttggaag aggtctaaaa
    18601 cataatatac cattaacatt tttgtcatca ttaactctct ttaatacatc aatatattcc
    18661 tcttgtgtta tatcctcagc taactctaac acttctgttg ttatacctat gttttgacat
    18721 ctctttagtg ctcctctttc ataagataaa tcatcacttc ttgccccaac tctaactatt
    18781 gttagtttag gatttatacc ttctttaatt agtaaatcga cttcttttat taattcttca
    18841 ctaatcttat cagctacagg ttttcctttt ataatttgtc ctttagttga cattccttcc
    18901 atacattcac aactccccta caagcaaact taaattaatt tattctatat accacatatt
    18961 aataacatag tatctacttt cgtcatttaa aaattatttt ccaagagctt tttcaacttt
    19021 ggcataaact tcatcacaaa tttttacacc atcttcaact aacttatcta cttctgtttt
    19081 taccttatta acatattctt tatcttgtat agaatttata tttataacta catttaattg
    19141 tccaccaagt atagcagctc ttaaacactg aactcctaca ccaacatcac ttatagcaag
    19201 tcttgaacct ttatcaacta aatcttcatg taacttaata gcttcatagc aaactcttac
    19261 tatgtttaat ggtacttcac aagcaacttt taatgccttt tccattgttt ctgcttttat
    19321 tcttttttct tcttctgttt ctttaggaag cccgtaagct tttgataatg gtaagaaaca
    19381 ttctgcatca tcatctatca tttttaataa atctttttct aactttcctg ccttacttaa
    19441 tatctctttt acactttctt catactcagc atacttcttc ttccctattg taaggttaca
    19501 taccatacta cctaaagcca tacctattgc acctactagt gcagcagctc ccccaccacc
    19561 tggtactgct tctttggaag atagtacttc tacaaaatca acacaagttt tatctgctat
    19621 tttcatgata atcctccttg aaaggatgta ggaaaatatc tcaaattgtt cgagatattt
    19681 tacgaactat tttaagatat ctcctactct ttatttataa attaatttcc ttagaataat
    19741 ccagctatta ctccattttc atcaacatct attttttctg ctgctggaac ttttggtaat
    19801 cctggcatct tcattatttc tccagtaagt gcaactataa atccagctcc tgctgaaatt
    19861 gttgcttgtc ttacagttat tctaaatcct gttggtctac ctaatttagt ttggtcatca
    19921 gttaaagaat attgagtctt agccatacat actggaactt tagtaaatcc aagtttttct
    19981 aagttagcta tttccttatc agcttcagga gtataatcta caccatctgc tccataaatc
    20041 ttagtagcta tagcatttat cttgtctttt atagataaat catcttcata gcagaatcta
    20101 aagttatttt cttcttcatc caataatctt aaaacttctt tagctactgc aattccacct
    20161 tctccacctt ttgcccatac ttcagaaaga gcaacattaa cacctaattc cttacacttg
    20221 tcttcaacaa gtttaagttc agcttcagta tcaagtggga atctgttgat tgcaacaact
    20281 gctggtaatc catatacttg agttatgttt tcaacatgtt ttaataagtt tggaagtcct
    20341 ttttcaagag cttctaggtt ttcgttattt aagtctgctt tagcaactcc accattgtat
    20401 tttaatgctc ttacagtagc aactattata acagcatctg gttttaagtt tgccattcta
    20461 cattttatat ctaagaattt ttctgctcca aggtcagcac cgaaaccagc ctcagttatt
    20521 acataatctg caaagtgcat agccattctt gtagctataa cagagttaca tccatgagct
    20581 atatttgcga atggtccacc gtgtacaaat gatggtgttc cttcaagagt ttgaactagg
    20641 tttggtttta aagcatcttt taataatgca gccatagcac cattagcttt taattgtcta
    20701 gctgttacag gttcaccttc gtagctgtat ccaactatta tgtttcctaa tctttctttt
    20761 aaatcagata tatcacttgc caaacagaac gctgccatta tttcagaagc aacagttata
    20821 tcgaatccat cttgtctaac tgctccatca ccttttttac ccataccatc aacgatattt
    20881 ctaagttgtc tatcgttcat gtctacacat cttctccaag ttattttctt agggtctatt
    20941 ctaagtgcat ttccttggtg aatatggtta tcaagcatag cagctaaaag attgttagca
    21001 gctcctatag cgtgaaaatc tcctgtgaag tgtaagttta tatcttccat aggaacaact
    21061 tgtgcatatc ctccaccagc tgctccacct ttaacaccaa atactggtcc tagagatggt
    21121 tctcttaaag caactaatac atttttgtct agtttagcaa atgcatcagc aacacctata
    21181 gtagtagtag tttttccttc acctgctgga gttgggttta tagctgtagt tagtattaac
    21241 ttagcttttt taccagtctc tctttttagt agattgtagt caacttttgc tttgtattta
    21301 ccataaagtt ctacatcatc ttcacctagt cctaattttt ttgcaacctc tctaatatct
    21361 tgaggcttag cttcttgagc tatttcaatg tcagatttaa atcccataac tgaatctcct
    21421 cccacaacct aaatagattt aaaatataat ttttgtaaaa attgtattaa tatgtaaaaa
    21481 ttatatttta taagataatt atacatgtaa aattacttaa aaactagcta ctaagctatt
    21541 aaaatattca aacttaatag ctagttattt atctaattac ttacttatta cctatctatt
    21601 gcctatgtct aacctttaac caacttaaaa tcccaaatca aaatttctat ctattttatt
    21661 tatccttaag tgacattaat ttatctttta ttgctttaat ctgttctttc gttgtttcac
    21721 tagaatcata tggagattga ttagctctat cagaatcaat aacatcttgg ttataataaa
    21781 taaatcctaa agattctcca tcttctaagt tttggattat aaattcttca tcttccttat
    21841 ttctaatttt atttcctact acaaatactt tatttacacc tatatcatgt ccaagtttct
    21901 taacttttct ataagtctgt agacttctct ctcctggttc tacaactact ataaaagcat
    21961 ctacaccttg ggctgttcct cttcctaagt gttcaattcc cgcttccata tccataacca
    22021 ctacatcttt attttgtagt attaaatgtg agcagagtct cttcaaaaga acatgctctg
    22081 gacatacaca tccagtacca ccagaatcta cggttcccaa tgtaagtaat cttactccat
    22141 tgtattcttt acagaaattt tcagggatat cgtctacttt aggattcatc ttaaacattt
    22201 ttccaaatga acctacagaa gcagctgttc tatctgaaac taattttttc atttctgata
    22261 taggtactat tgattcatat acttcttttg gaaaacctag tgctaaagct aaattagcat
    22321 cagggtcggc atcaacagca acaactctgt atccgtcttc agcaaacatc ctagagagca
    22381 ttgaagaaaa agtcgtttta cctactccac ctttacctgt tattgctatc ttcataatcc
    22441 ctaccacctt ttattactat ttgcttttta gtagttaatt ttacagttaa atatttttta
    22501 tactctatat ttttatacac taaaaataaa ctacagctag tccttacagg aaccaattaa
    22561 atttacccta ctagcttttc aaagcagcat gtatagtttt attattcttt aatattgttt
    22621 atattttatt tattcagtat attcattata ttttgatgtt ctttacttta atatttgact
    22681 ttattcaact ttattcaacc agaaacttct tataaaaacg tttattctat aacttcacta
    22741 ttttttaagc ttcagccttt tctgccatct tttcttcaac taatgcttca aagtgaacac
    22801 gttttttctc tatatcttct attattgtag ctgctaactc ttttggattt gtatttacag
    22861 taaatgtagc tccaacttta tcttttaatt cactagttaa tatatccact gctcttgaag
    22921 aaccagctat tggaggcatt attcctaaat aagtgtctat accagaagct acaacatagc
    22981 atcctatagc aactgctttt tctgacatcc actctggagc tatacctaca actggaaggt
    23041 cacacatatc catatctaag taattagctg ctgctccaac taaatctagt atacgactta
    23101 tatcaacaca agaacccata tgtagtactg gtgggattcc tactaattta cagacagttg
    23161 caagaccttt acctgcatat ttttcagccg catcagattc cattaaacca aacttagctg
    23221 ctgctgaagc tccacaacct gtagtaacta ctattatgtc gtttttgatt aattctttca
    23281 ttattgttac gtgagcctca tttgaaactc ctttagcatt gttacatcca actacaccag
    23341 ctgctcctct taatactcca gcttttagac agtctgttaa tggcttaact gttcctgctg
    23401 ggtctatatg agagtttaca actctgtcta attgatttac tactgcttct acactatacc
    23461 caactgttgc ccctgatttt aattcaggta taagtacttt tgatttatct ctatttttaa
    23521 agtttaaaat agcttcttta actattgctt tagcatcatc aagtgcttgt tcttctctaa
    23581 attcaatata agttgaacct gttatcttag cttttggaga agttgttaca aatttagtat
    23641 ggtagcaatc tgcaactcta gctaatgcag ggaatataca ttgaacgtca actattacag
    23701 cttcaactgc tccagttatt attgcaagtt cttgttgatg gaagtctcct gctattttta
    23761 caccatgtct cattgtaatc tcattacctg tacaacacat acctgctaag tttattccat
    23821 cagcaccaac ttctttagct aaagctacta agtctggctc ttctgatgct aaaactatca
    23881 tttctgataa tgctggctca tgtccatgta atattatgtt aactttattt tcttctaata
    23941 ctgctaaatt cgcctctgtt cttcttggca ctggagttcc aaataagata tcgcttagac
    24001 gagtacctat catagaacca ccccaaccat cagccataga agttctaaga ctcatgtgta
    24061 ttaaactatc tatatcacca gtacatccta tatgagtaga gtgcattatt gttgcaattt
    24121 ctctatctat agctcttggc tctatagcgt attctttcca tattttttgt cttggttctg
    24181 gtgcattttt tataaatctt aatgtcccaa aaggttttcc aaattccatt aatgcaattt
    24241 ctgccacttc atgtgctaca tcatagatat ctctaccttc tgtctctaca tcccattctt
    24301 tagctaaagt aattaactta gcttcgtctc ttattttata gtttccattt ggatcagcta
    24361 atgctaatgt atgtgctata tctctagcat ggtctgaatg ggcagctgtc ccaccagcaa
    24421 ccattcttgc aaagtttcta cctactatag tatgctcatc tgctccacat aacccccttg
    24481 gagtcttagg gcttattcta catggtccca ttccacatat tctacagcaa acaccttgaa
    24541 gaccaaaacc acattgtgcc ttaaagcctt cttttctatc atacattgtt tctacgcctt
    24601 cttctctagc tttagctatc atcttctggc tgtttaaatc aattgttaac attttttcat
    24661 ccatattcct atcccctttt tttgattttt aaaatccccc aggtgactga cttcatgtca
    24721 taaaataata aaataaatgc tttctaagtt gattatatac taattttcag tgctttgctt
    24781 tagatttaaa tattttttta acaaaattag ataatacata aaaattttat tttctacact
    24841 taatttaagt tgattttatc gcttgttttt ggctatttgt taatatctaa acctactttt
    24901 ttgatacttc agtttaatta aattatgttt tgattttttt aaatcaaatt tgaaattttt
    24961 ctagtcattt ttatattatt gcagattgtt tgtgtattta ttatctttca ctactgtttg
    25021 gacttattac agatttttac tccctaaact taattagtaa aaacttctta gaatagcatt
    25081 ttttggacta atttttttga atttttttat ttttcctact ttaatattaa ttatagcttc
    25141 aaattgaaca tttgtatata ttcatttgaa gctataatta ttttttatat aaaacttttt
    25201 taatacagca ttaatttaga cattttgact attttttcta ctttaattca tttatttcaa
    25261 gcgatttatc ctatttcata tttttagcta caatagcccc atatgcaata gaatataatt
    25321 tagattcatg tgaatcagct cttgacacta aaactatagg agcttttgca cccataatta
    25381 tacctgctga tttagagtct gccatatatg taaaggcttt ccctattcca ttaccaacct
    25441 ctattgttgg aaccaatatg atatctacat ctccagatac ttcactttta aaccctttta
    25501 tctcactagc ttcttttgaa acagctaaat caaaagccag aggaccttct acaattacat
    25561 tttcaccaaa ttctccttct ttacaagctt ttgctaacat atctgcatct acagttgctt
    25621 gcatcttagg atttactttc tctttagctg ccaaacatgc aacttttaca gtttccattc
    25681 ctaaagcttt tgcagctttt atagaatttt ttaatatctt tgccttttgt tcataatcag
    25741 gagctatatt catacctcca tctgtagtta taagtaattt atggtatttt tctaactcat
    25801 ataccataac atgacttaat aaactatcag ttctaagacc atagtcttta tttaacactt
    25861 cttttaatat gacagatgta tctaataatc ctttcattac aaagtcagct tctttacttg
    25921 atactaattt aactgctatt tctgcacact ctttgatatc atccttatcc actattttga
    25981 taccacttaa atcaaatcct atttcttttg atatttctct tattttgcct tcatttccaa
    26041 ccaatatagg agtaattata tttctttcta cagcatcctt tattgccatt aaaacttctt
    26101 tatcatgagc tgctgctaca gagagtacaa ttttctctcc tcccttaagt tgttctaaaa
    26161 tatcatctaa tttttttatc aaaagtaacc cctcccttat atatatttaa ctaaataata
    26221 ccatatttgc tatacctttt ctcaatttat tttttatgtt ataaagaatt tatataacta
    26281 aaagacagtt taatatttta ttctgggatt tcatcaataa ctcttaaaag ctttgcagga
    26341 acaccaccta caatagtatt ttctgcaaca ttttttgtta caactgctcc agcagcgata
    26401 attgcattat ctccaatagt aactcctgga actacagtta cattagcacc aatccataca
    26461 ttttttccta ttgtgattgg agaaggataa gttgtaccac gtttctctgg tgcaatacca
    26521 tggttaagtg tagccaatac cacattatgc ccaatttgcg tattatctcc aatctcaatc
    26581 cccccctggt cttgaaaatg gcaactgcta tttataaaaa cattttttcc tattgtgatg
    26641 ttttttccac aatctgtata aaatggtgga aacataaaaa atgattcatc tacctttttt
    26701 ccaattaact cagaaaataa actacggact tcttctggtt catgataaga agaatttaac
    26761 ttacttgtga ttttcattgc ttcattactc aacttaacca taaattcatg tatttctgat
    26821 ttactagaaa caggttttct ttgattgata tgtgttaaaa attcattgat attcatttta
    26881 tacatccttt caatatgtgc tttaatttat ttgtacattc taaataatat tttaataaaa
    26941 attaatttaa tttattttta ttaaaatatc attgcttctt ttagtgtaat atacaattat
    27001 atctatatca aatatctata ttaaatactt aactatactt aaaaagcatt taaattgata
    27061 taattatata ataaataagt taatattgag gagagataat tatggaaata agagtattaa
    27121 gatatttttt agcaattgca agagaagaaa gtatatctgg tgcggcaacc tttttacata
    27181 taacacaacc aacactttct agacaaatta tggaacttga agaagagttg ggagttaaat
    27241 tattggttcg aggaaataga aaaatcacct taacaaatga aggtaggctt ttacataaaa
    27301 gagcagaaga aattatatca ttagttgata aggttgaatt agaatttgac aatcccaatg
    27361 agattattag tggtgatata tatataggaa gtggtgaaac tgaagctatg cgacttatag
    27421 ctagaactgc ccatcatttt catcaaaaat accccaatgt aagatatcac ctttttagtg
    27481 ggaatgctga tgatgtgact gaaaaattag acaagggact gcttgatttt ggtattttaa
    27541 ttgaacctgc aaatatacag aagtatgatt atgcacaaat tcctgctact gatgtatggg
    27601 gtttactaat gcgtaaagat agtgcccttg catcacaaag cacaataaat ccaaaagatt
    27661 tatgcaatat acctcttatt atttcaagac agacacttgt tgataaagca atttctaaat
    27721 ggcttcaaga cagtaatgaa aaactaaaca ttattgcaac atacaattta gtttataacg
    27781 cttctctttt ggtagaagaa ggtctgggct acgctctgtg tttagataga ctcgtcaata
    27841 ctacagaaaa cagtaatctt tgttttagac ctctatctcc tcctttagaa gctaaattaa
    27901 atattatatg gaaaaaacat caaatatttt caaaggcagc agaaaaattt ttacttcaaa
    27961 tacaaaaaga tttttcttct atcatgttgg atacatctaa gttggataca cctaaagaaa
    28021 aataaatcat aataaaaagc gagagtgtct caaaatgaac tttgcactgt ataaggcact
    28081 cttttttata caaagtaaaa tatatgttca tcatttcagc actaaaaaag aggcttatct
    28141 ctattttgag atagccccac ttaaccaata aaattaaata caacttaaca atatccttac
    28201 ttatttattc caagttctct tagacaagca atcgtaattt catcttgtgg atttaattca
    28261 taagctcttt ctatatagta agttgcatca tcaatatcac cattatttaa ataagccatt
    28321 cctaaattgc aaagtatttc tgggtcttcc tttatcttag ccgcttgttc aaaatactct
    28381 attgctttat ctaaattatt cttataagcc tcacaaagcc ccaattccac tatagtatcc
    28441 acctgattag gctttattat caatatcttc tcaagataca tcttagcctg ctctatttcg
    28501 cccataccct tatatccaag tgctatcata aacagcaagt tccaccaatc catatgttct
    28561 tcttctaatg gaagtaactt ctctaatcct tccttaaact taccttggaa tacaagtgta
    28621 tatccttctt catattgaac tttaaagtcc atcttgccca gattctcttg tacctcagct
    28681 actaaatcag catctaaccc tagctttaac gcttcttccc aagtcagctt agtctttaaa
    28741 tattgacctt gattataata atggtagcct aagtgataat aacctaatgc aaaattctca
    28801 tcaacatcaa ttaccttttc aagcttagcc atggcttcaa gtaagaatgc attcattgcc
    28861 ttatcatcca tatctttttg atactgttta gctatctctt ggcaaacaat cgcatagtta
    28921 tacattgcat ctaaatcatc tggatacatt gttataaatg cttttatata tattaaagaa
    28981 tctgtatgtt caccaatctc aaagtatttt ctcgacatat atcccatata cgatttcaaa
    29041 tccaaatcaa tatcttttga taaagcattt aaaaacttct catactctgc attgttttta
    29101 aacttactat ctattccaat tatatatatc attgcatctg ctatagacat tgaatttaac
    29161 ttatcttgag cagtcttttc ttttatccct tttactaata cctcattttt tataggtaca
    29221 tctaatccac cttttggcac ttcatagctt tttaatttaa actctccacc atgttttata
    29281 gttataaaag ctaattcttc tgcttttttt agcaaatatt tttctatctt aaaatccatt
    29341 ttatcctcct aaatacttaa atctctactt tatgagtttt agatttctca aatatttttt
    29401 tatcatcata ctcaaataag actacatttt ctacttctct tatttctgta tttatatcat
    29461 tttttaaatc cacaacatta aatctaaatg gctcaaaatg aacatatttt attatatgtt
    29521 tagctggttt atcaacaaaa ctaggtaaat atgttgataa gttatcatta tcttgtaaaa
    29581 atacatggca tctattctta aaatcatcca catctagctt attaaaaaac tgtggtatat
    29641 ttgatgtttt acccagtgat atatagtcat attttaatat atcgttgaat acatcattat
    29701 ctatgttgat tttttcatta tagaaatcca tcaatatttt atataactga ttctttcctt
    29761 gtgccaaatc aaagtagcca ttttcatcaa aatacttggc aaaaacctca aagaatttaa
    29821 aagggctttg tttataaaat ctgcctataa tatatctcat agataacaca aagtttttag
    29881 aattgtagta tctctccagt atatcttcta tatcctttag cttcaaagtt tcttcatagc
    29941 ttatataatt attgtataaa aactcatatg gagcataatc cttatattta aaaccatgct
    30001 catctgcaac ttttctcata cctgtccctt taatcatctt taaaaagcct aattgcaaat
    30061 gctctatacc tagattaaat acatcattaa aagaattttc aaagctctta taatcttcat
    30121 aaggcagtcc tgcaattaag tctaagtgct gatgtatatt tctatatgat gctacagttt
    30181 gtacaacatg agacaacttt ttaaaatcat cccttctacc tactgcatca agtgtttttt
    30241 cattcgttga ctgaacccct atttcaaatt gaaaaagacc ttctttacaa tcagccaaaa
    30301 actcaagcat cttatcatct atcaaatacg ctgtaacttc aaaatgataa gttgtatatc
    30361 cattatcatt tttcataagg aaatccatga tttccatagc cacttttcta tttgcattga
    30421 atgtcctatc tataaacttt atctgtgata ctcttgcatc tattaaagct tttaaatcac
    30481 tctttatcct gtctaaacta aaatacctta gacctggaat agtagatgat aaacagtatt
    30541 ggcagttaaa agggcaacct cttgatgatt cataatacac tattctgttt tcatattctt
    30601 ttggattcaa gttttcataa ggactaggaa tctcatccaa atcttgaagt aattccatag
    30661 gcttattcac tattatctca ttgccttgtc tatatacaag tccatttaca tcatttattt
    30721 ccatctttcc ttgcaagtga agtactaagt ctctaaaaat taattctccc tctccataaa
    30781 gtatataatc cacaaaatca tactttttca ttgcctcata actatcataa ctcacttccg
    30841 gtccgcccaa agctatttta gtattaggtc taattttttt tagattatca caaagtttaa
    30901 ctatatctcc cacattccag atatatgttg agaataatat tatatcatat tcatttttat
    30961 atatatcctt taaaatatag tccaaatcgt tatttatcgt gtattctttc atattaactt
    31021 ctatcaaatc tcttacaaat tcttttagat atcttatagc caaatttgta tgaataaact
    31081 tggaatttaa tgttgttaat aaaattttca aatatttctc acctcttttt aagattatac
    31141 tgtctttttg taccccttat aggttaaaat agtatatgta aagtctaaat aatatctata
    31201 taattaattt attaatttca agtaatgtga ggaaaggaag ttttttatgg aattaattga
    31261 aaaagtatct atcaactctt tatcaaaaag agatttacta cttattataa agtctcttga
    31321 atacactaat gaaaatactc aaattagtga tttcattgac cttaggaata atatagtaaa
    31381 ggaattatgt tttttaactg acactaagga aaaagatttt ttagattatc tagaaaaaaa
    31441 tagcacactc taataaccta aaccttttta aactttaata tataaatacc tgatttagaa
    31501 tttattttta tgcttaatat attatcatta aaggttttcg acataactgt tatttgatta
    31561 aatatagggt ctactatagt attaccttct tcaagtctta taagacttct tgggtctatg
    31621 cttttattat atgccttaac tccaatttta tattttctca atttaacaac atattcggac
    31681 atagaaaaaa taaaatctat attatcttca tttaatttac tatcttcacc caaaatggat
    31741 aatatagatt tttttatgct attaattcct tcattttcta cagttctatc ttccatatat
    31801 acatcatata gaacatttaa aacatttaat aaaatacttt cttcatcata atcttcagat
    31861 ttcaaatcaa tgtacttatc aattaattgt attgctttaa tccttgccat ctgaaatttt
    31921 acaacatctg atttgtctaa ttctatagaa aatcctatta tcttgtcttt aatactctgt
    31981 agtaattcga catactctat tttactatta tttataagat tgtgcataat ggaattatac
    32041 actacagaac ctaataaata atcaaataaa agttcttctt ttttaaaata aataacatac
    32101 ttttttatta gtttaataac ctcatcttca cttttttgaa tattagtatt tgataaaatt
    32161 agtggtaata aaatatttaa aaaattgata tttcctattt tttctccata gaatctatag
    32221 taaataagtt catctctcag tctattttcg tctatataca cacatctttg cgttttatca
    32281 tctattaagg attttgtatt tattatagtt aaatctttaa gagccctact tgcactacta
    32341 aaagaagatg tgtagacttt ttttagtaat tcgtcattca taaatatatc ctttctgaaa
    32401 aattgaatgt aaataaattg ttattaaaaa aaaagagaca gcccgtttaa gaagctgtca
    32461 tatatagggg gatagtagtg tacaaaaatc attgtaatct actatctata tattattata
    32521 cgatactact tttttatatg taaagcatca gttttcgaca tccctatatt ttacagttat
    32581 acaaattgcg tactaagttc aattttattc tttttatttc aacattttat catgctacat
    32641 tctttcagga gcgtgcattc ccaacaattt cagagaattt tctaaagttt gttttgttgc
    32701 taaaactaat gcaagtcttg cttttcttat ttccaaattt tctactaaaa ttggattgtc
    32761 atgataaaat ttattaaacg cttgagctaa atctagcata aatctagtca ctatatgtgg
    32821 ctcatttttc tttagagcaa gtaaaatatt cttattgaat gattctataa ctctaagaac
    32881 ttctgcacta tcttcatcag ctaatagact ataatctata tcagaagtca cttctacttc
    32941 tgcttttctt aaaactgcac agcatctagc atgtgtatac tgaacatatg gtccagtctc
    33001 accatcaaaa ctaagcgttc tttcccaaga gaaagtatag tctttaattc tactattaga
    33061 taactcttgg aatactactg ccccaacacc tacttgctta gatatttcat ccacattttt
    33121 agcatttggg tttttagcta gtataatttc cttagtttta tctatagctt gatttaaagc
    33181 atcttctaaa aatactactc ttccttttct agtcgacata gtaccttctt caagtgcaac
    33241 cataccaaat ccaacatgaa ttaaatcttt tgcccaatca tatcccataa gctcaattac
    33301 cttaaaccat tgttggaaat gtaagttttg ttgagaacca actacatata tacacttgtc
    33361 aaaatcataa gtttcttttc tatatattgc agcagctaaa tctcttgtca tatataacgt
    33421 tgaaccatca ttcttagtaa taagtgctgg tggcatttta tactcttcta aatcaacaat
    33481 tcttgctcct ttagactctt ttaaaagatt tttctcttct aatagttcga taactctgtt
    33541 cattttatca gaataaaaac tctctcctgc taaagaatca aattctatat ctaaaagttt
    33601 atacactctg ttaaactctt ttaaactctc atttctaaac cattgccata attcttgtgc
    33661 ttcttcgtca ccattttcta atttattaaa ccatgctctt gcttcatctt ccatttctgg
    33721 gtgttgttca gcttcatcat ggaatcttac gtataatttt agcaattctg gaattggatt
    33781 agactctact acttcttttt ctccccactt tttaaaggcc actattaact taccaaactg
    33841 agttccatag tcacctaaat gatttattct tatagtcttg tatccttgcg aatcatatat
    33901 cttatataaa gcattaccta taactgtagt tcttatgtgc cctatatgga aaggttttgc
    33961 tatatttgga gaagaatatt caactattac agttttatct tttccaaaat cactatgtcc
    34021 gtaattttct ttttcgtcta aaactttttt aattacagtt tctgctaatt gagatttatt
    34081 tacaaagaaa tttacatatc cgcctaattg tattactttt gttatctctc cacttggttc
    34141 tatactttcc gccaattcac ttgctatcat gtttggagct tttctaaaca ctttggctaa
    34201 tttaaagcat ggaaaagcat agtcccccat atccttattt ggtggaactt ctataagtgc
    34261 ctctatctct tctttcgata aatcttctat tttttcttta aggcaatttg atattgccac
    34321 cttaaaatct tgcattctgt tgctcctctc tacattgttc ctggtttata ttgtgcttat
    34381 ctctttatgt taaatcaaat ttcgtgtttt ttcaagtatt tcattacagt gtaaaacact
    34441 ttagtaatta tgttttctgt atatattaaa aatactataa ttgcagtttt taatcacact
    34501 attattgatt ttttataaga cttttttcta ttttattgtt tcttgtacta tccagttgac
    34561 tttatatact ttttttaatc ttttatctat tttcatacgt caatgctaat tttatatact
    34621 tttcttttaa tcttttatta attttcatac atcaatgtta actttatata cgttttttta
    34681 atcttttatc tattttcata catcaatgtc agctttatat acttttttta ctcttttatc
    34741 tattttctat aaatcttttt cactcttaac ttttattcct tcttcttcta atagccttgc
    34801 tgttatacct ttccctggta tctttttacc tgaaaaacta ccatcatata ttagattaca
    34861 tccacaagag gggctaccct cttttagtat tgcttcgcta catacatata aatttgctat
    34921 ttttagagtt tcattagccc ctcttacaaa gttttctgtt acatccttat tatctttagt
    34981 aattactttt ccatcaacta tctcagcagg tggtcttggt gtagatagac cacctaactg
    35041 ctctgggcat acaagtgtaa attctttgcc ttttaaatat tccttgactt tttcattatc
    35101 attattatcg ccactatatt tacaatttat tcctattaaa catgcagata ccaatatcat
    35161 aaaaatgtgc tcctatacaa attatatata ttctcataca aattactaat gtattcttat
    35221 acaaattatt ttaatttaac aattgttact cctgctccac cttctccata ttgaccacct
    35281 ctttgtgatt ttacatgtct atttcttctt aaaatgtctt gtagccctgc ttttaatact
    35341 cctgtaccta ttccatgtat tactgttaca gactctaaac ctgccacata tgcatcatca
    35401 agatactttt ctacttccat tatagcttct tctaggttta accctctaag gtcaacttca
    35461 ttttttacac taccagattt tgaccttata atgtttctcg ttgatttagt tacatttgtt
    35521 gaaacatctt tgcgtgtttt ttgtaaagat ttaaaaggta gagtcatctt cattatacca
    35581 atttgcacta ctgcttcttt tctcttttta tctactgaaa ctacactacc attttgattt
    35641 aaagttatta ccttaacttc ttcacctggc tttaagtctt ttatctcctt atttgatacc
    35701 tttggaacta tcatactttt tactgttggc tgaagacttc ccatagagct ggtcaattct
    35761 tttctaagtt cttctatctt acgattcttt tcttttgaag ctctttcttg ttcaagactt
    35821 ctaagttctt ttattataat atctacttct tctttagctt gtcttataat tgaaaatgct
    35881 tctgatttag ctttttcaat catcttatct ctttgactga ccaatttttc aagtttttcg
    35941 tcatactcta ctttcagttt ttcaatctct tcttttaatc tttcagcttc ttctctatct
    36001 tctactgctt tgattctatt cttttcaaca ttctgtaaca catcttctaa agctatgttt
    36061 tctgtattta tatattcttt agcacgtgaa atcacataat cacttagccc aagttttcta
    36121 gaaatctcaa acgcatttga ttttccggga acacctataa gtaacttata tgttgggctt
    36181 aaagtttcta tatcaaattc aactgctgca ttttctacac ctgatttagt aagagcataa
    36241 tttttaagct cactatagtg agtggtagct atacatttag ctcctacaga atttatatct
    36301 tctaatactg ctattgcaag ggcagctcct tcaacagggt ctgttcccgc tcctaactcg
    36361 tcaaatataa ctaaagagtc tgctgttacg ttttgtaata tagatactat attcgtcata
    36421 tgagatgaaa atgttgatag actctgttca atactctgct catctcctat atctgcaaat
    36481 acattatcat atacacacat actacttcca taatcagcag gtatatgaag tccactttgt
    36541 gtcataagtg caaatagacc tacagttttt attgtaacag tcttaccacc tgtgtttgga
    36601 cctgttatca ccaatgtatg gaagtctctt cctaaataaa tggtatttgc tactaccttc
    36661 tttttatcca gcagtggatg tcttccattt tttatgttta aatatttatc ctcatttaaa
    36721 gttggttcta ttcctctcat ctgtatagaa agttttccct ttgaaaatgc aaagtccaat
    36781 cttccaagta tctcttgatt agagattaaa tcttcgctca cttctccaac catagcagaa
    36841 agctctgata aaattctttc tatttcttct tgctcaccta gttttaactg tctcaattca
    36901 ttgttcatct ctactatagt cattggctct ataaaaagtg tcgcaccaga agatgactgg
    36961 tcatgcacta taccagctac ttgcgaccta tattcagatt ttacaggtac tacaaatcta
    37021 tcacctctta gagaaattat tgcatcttgt aagtactttt gataagtagt tgaacttatt
    37081 attgaattta attttgacct aatggattga tttttttggg ctattcttct tctgatatct
    37141 ctaagtgtac ttgaagcatt gtcagatatt tctacttcac taattatagc attgtatatc
    37201 tggtcttcta tgtccttgta tacatataga gaatttgata gagattgtat tattggataa
    37261 ttaaaatctt cttcatcact actagataag ctatttctta atcttctagc cactcttaag
    37321 gtatctgcta tcataatcag acttcctggg tcaagagaag cacctatata agctctcttt
    37381 accttatctt ctatatcatg aataccttcc aaccctactg aacctctctt tataataatt
    37441 gattgagctt cactagtttc ttctagcata gatttcactt ctacaaaatc agtatttggt
    37501 actaaatttt ctatatattt tagacctaaa gaagatgagg ctttcttctt taacaaatct
    37561 attattttat tgtattctag aactcttaac gatttctcat tcattttcta tattcaccat
    37621 cctcgatatt ttaacttttt tatttaattt taaaaatgta tgttttcaag catttccaat
    37681 aaaaactgca agttttattt ctttttcatt tctaaaatta ttatattttt tctcttttag
    37741 atatatttta cacatatttt gtattatact atgtatgtag ctatctatat attatacata
    37801 aacatgcttt atttgtaata taatctgtta aaaaatcttc tttaatattt ttaaaggagt
    37861 ttattttagc ttataaaaaa aaatagctac ttatatagat aaattaagga ggaacatgag
    37921 tcatgggaaa cgtattagaa ggattaaaac cagaaagtgt ttttaaaaac tttgagaaaa
    37981 tatctcaaat tcctagaggt tctggaaatg aaaaaggcat aagtgatttt ttacttagct
    38041 ttggtaaaaa tttaggtctt gaaactattc aggatgaaag cttaaacata ataataagaa
    38101 agcctgctac aaaaggatat gaaaattgtc caggtgtagt tcttcaagga catatggata
    38161 tggtttgcga aaaagaaaag aatgttgaac atgattttct taaagaccct ataaaattaa
    38221 gaattgatgg tgacatgata tatgctactg gaactactct aggagctgac aatggtatag
    38281 ctgttgctat gggtatggct atattagaag ataatacttt agagcatcct gcactagaag
    38341 ttttagttac tgttaatgaa gaagatggta tgaatggtgc agatgcacta gaccctagct
    38401 taattaaagg tcaatatatc ctaaatatgg actccgagga ggaaggatac ttattagtta
    38461 gctgtgctgg tggtaaaact tgtgttgtta gtttacctgt tgaatacaag gaagttaaag
    38521 gtgacaaaca aggattatta gttgaggtta ctggactatt aggtggacac tctggtatgg
    38581 aaattgtttt acaaagagca aatgctaaca aagcaatagc tagagtatta agtgtattga
    38641 atgttgatta tgaattagct tctgttgatg gtggtactaa gcacaatgct atacctcgtg
    38701 aagctaaatg tgtaatagct gttaataaag ctgatgttga atctgctaaa aaacaaataa
    38761 atgacatttt aactgcattt aaacatgagt ttactacttc tgaccctggc atgacttact
    38821 cagtagctga aacttctgta gacaaagttc ttactaaaga ttgtaaagaa aaagttgttc
    38881 aaatgtcttg cttaactcct catggagttc aatcagtaag tcttgatata gaaggattag
    38941 ttgaaagttc tactaacttc gctataattg aaactaaaga atctactatt gaatttttaa
    39001 cttctgttag aagttctgtt atgtctatta gagatgaaat cgctgataga ataagacttt
    39061 tggctcaagc tttaggtgct aattacgacc ttatagctca atacccagct tgggaattta
    39121 aaaaaggctc aaaattagaa aaaatatgca gtgaaactta tgagaaatta actggtaaag
    39181 ttcctactgt tatggctcta catgctggtc ttgagtgtgg attattactt gataaacttc
    39241 ctcatgctga agctatctct ataggaccag atatgtttga tgttcatact ccaaatgaac
    39301 atgttagtat cccttctgta gctaatgttt gggattatgt tattgaaata ttaaaatcta
    39361 tgaaccaata ttaaatttaa gttttgattc taagtagatt ttaattttaa gtaatctata
    39421 taaaattaac tctgtaaatt aatactactg ttagtataat atttactttc aatattaact
    39481 tatagaaata aagcctaatg atagataata acatgtatca ttaggcttta ttgtaattta
    39541 aatattatta agctttattc tgttttaaaa ttaattctta tactatttta aatctatttc
    39601 ttccttgtct cttagaatca taaagagcat tatctgctat ttgatacagt tcatcatact
    39661 cttgaccagg ctttgctata actaccccca tactaataga taaactttct tcatttggca
    39721 acttaatgag atgaatatga ttgtatacat cattacattt attaataagt tcttccttat
    39781 ttattatatc attgatataa acactaaatt catctccacc caaacgacct actatatcat
    39841 tttttgcaaa actttcatca agtacctttg caacatcaat aagtacttta tctcctaaca
    39901 agtgaccata agtatcatta atcgacttaa aataatcaat atctataatc aataaagcac
    39961 catttttttc agttgataaa ttcaacaaat tttgtgcagt taattttcta atatatgctg
    40021 cattataaat attggttaaa ctatcccttt gagccttatc taacaatcta ttcttttcct
    40081 ctttttcaga atcaatatta catattttcc cgattacata cattggtatt tttctatcat
    40141 tataaattat ttttgaagta actctaatcc aatgaaaagt atcatcaagc ataatagaat
    40201 acatttcagt tgttccattt tcttgtgatt ttattacatc aaaaaacttt ttattatatt
    40261 ctttccttat tttatctctt tcattttcta tattgccatt ttcactttcc ttatcatttt
    40321 tatccaaacc ataataagtc tcaataccct tttctggcat agaaattatc aatttatctt
    40381 gcttataatc atactcaaaa aagtattcac ttgtcatttc atacaactga gtatgttttt
    40441 tgagttctaa agatatcttc tcgttcatac ttgtacgagt atgcaaacca aatgccataa
    40501 atccaataat acaaattaat aaactggcta caataaaaat agtctcaatt ggattctgtt
    40561 gcataatata aagcaacgag aaatcttgtc tatatgtagt attttggtag acaattgatt
    40621 gcattttttc tgatgaaata cttataatag ctttattaaa tatattcaat aattcttgtt
    40681 tatctttttt aattatacca aaacaaatac tctttgcttt ataagtttga ggaactagac
    40741 gcacattttt atacttaggt tgattcatat agtactgcat agaataacct tcgccataag
    40801 tgtaatctgc atagccttta ttaacagctt ctatacaatc ttctaaatta tcataccaaa
    40861 ctatattctc ttcaaactga ccataataat taataccttt ggctagagct acacgttttc
    40921 catctaaact attctcattt aatttattat taatcatcat agtatattga gtagaaagaa
    40981 aaggccgaga catagctata ttatgttcac gagagtattc ataatcataa atcattcctg
    41041 ctacaatatc aatttcacgc tttttagtca tgttttctaa atcttcttga gattttacag
    41101 gcacaagttc aaattctaag cctgttttat cacatatatg atttaacaaa tcaattgata
    41161 tacctcttaa ttcacctgta tctttatcta tattttgaaa tggtggtttg tttaaaagta
    41221 ctcctacttt taatttttct gtctctttaa tgtaatcagt ttcttcatca gataaaaaca
    41281 tttgattatt ctctggcgta aagtatttct catataaagt agttgaaaaa tatgggtcct
    41341 tctgttcaat attattaatt gctgagttaa gcttttgagt taaatttgta ctacctttag
    41401 gagtaatgaa ataaaatggt tttgatgaaa attttgctac agtacgtaca ccttctattg
    41461 ggttcatact cacattaaga agtacatctg ctttaccact ctttagtgct tctagttgct
    41521 catcatttgt tttacaatat accaattcag gtgacataag attcatttta caatactcat
    41581 ctaattcttt aagtctagta tcagaatttt ctattacagc aatacggatt tttttcacat
    41641 cttgtgaact cattatgtta tcaatatctg tattttcata taatgcttgt agtactgtat
    41701 ttgctactcc ataattattc cctgcataat tatatatttt ttgcattggt tcattgtata
    41761 acatacctcc cattatatca atttcaccat tttctaacat cttcattaat tttgtaatag
    41821 attcatttat ctctccagga acttgaacaa attcataatt ccatccagta tactgtgcaa
    41881 tttctagtaa atattcatat gtatacccaa aatagttacc ttgctcatct atttcagtta
    41941 aacctttttg aataggataa gcaacacgaa tggtacgatt atcattacct atagcgttga
    42001 taccaaaaga aaatatgcaa gaagtgaata tagatgtaat tacaataatt aataaaagta
    42061 atttttttaa attcactaca caatcctcca tatagttaag atttacagta taaattactg
    42121 cataaacttg tagacatgta aaaatagatt agtatataaa aggatatata aatctactta
    42181 gaaatttaaa tatccttaat atatacaata tatttaaatt ataactcatt tatatgtcta
    42241 ttacaacaaa tttaccctgc taaataccta ctctatatcc cataatttta caagttgtag
    42301 tgaagtttaa gttctatctc acctcaaatt tagcagtgtt tctatttaca gttcttttat
    42361 atttcacatc tggataaccc aatataaatg aagtgataac agcttcgttc tcgctcattc
    42421 ctaacagttc ttttatcttt ggctctacat ttgatgccat aacaaaaaat ccattataac
    42481 aaattcctaa tccttgagaa taggccataa gttccatatt agatgcagcc agtcctccat
    42541 ctacataagc acttccacct agagacatat cacctacaac taccataagt agtggggcat
    42601 cataaaataa agaatcattt ccgttctctt tgtatctttt gtacattttc ttaaatgtat
    42661 ttttatagat agaacgtaca gggtcattgt catcagtatc taatgccaaa ttataaagac
    42721 cttgtattgc cagttctttc acttctttta gcttttcttt tactaatatg tatcttatag
    42781 gctgtctatt accacctgtt ggtgtgtatc taccagcttc cactatgttt tttatttttt
    42841 cttcttctac ctctatatct ttatattgtc ttatacttct tctaaattta ataaaattca
    42901 ataacttttc gggttctaat tcaaaacttt cacgattgta ttctattact tcatttttat
    42961 caaatccttg catttctatt gcatctgttg ggcaaattgc catacagtga ccacagttaa
    43021 tacagagaat atttttaacc tttgctttat catccttaac ttttattgca tttacaagac
    43081 agtctgattc acacatccca cagcctacac atttttctaa atttatgtct atcattccaa
    43141 attattcctc cttttaaaaa atttacttta gtataaaaca aatagttgtc taagtcaatt
    43201 tttatttttt taacaatata ctagccttaa ctacacttta atggagaaaa atatacataa
    43261 attggaactt aaattagaac ttattcaaag taaaagataa aagaagtaga tgtctaaaag
    43321 tgaacttttt agttaattag atattcttta ttgtatgaaa tcaagtggat atttatcatt
    43381 tcaacaataa aaaaggctta cctctatttt ggatatgtcc ctgtcaagta gacatactaa
    43441 aaaaaggcta tctttatgca ctgagatagc cccttttccc aactactatt gtttatctta
    43501 atattagtca ataaatttaa tttgctataa cctgtaagtt acattttaat ttgatgtaac
    43561 tttaaattac atttttaagt aatatcttta aattatattc ttaaaatata tcattaaact
    43621 acattttaat taatctaaaa actatatctt aattaatcta agaaacttac attataatct
    43681 tttagtttcc cattatcata tataatcttt gcactaaaga tatttccagt cttagcccaa
    43741 tttaagaaca gcctgtcacc ttcccacata tttaaatcga atattttatc attatcaacc
    43801 caaattaaat ttccttcttt acaatcattt aaatcttttt cagataattc accttcaaac
    43861 tcatctgaga aatacaaaaa catataccaa tcttcttcat ctttaaattt tggaaaagtt
    43921 atcaaccctt tatatgacaa tgatttaaga gtaagaccag tctcttcttt tacttctcta
    43981 attacacatt cttctggagt ctctccttgc tcaaactttc ctccaactcc tacatactta
    44041 ccttcatgta tatcatcttt ctttttattt ctgtacaaca tcaaagtctt gtcatctttt
    44101 tctatataac aaatagttgt aagtttcatg tatctcgcct tgctttctct tttatattct
    44161 caactaatta ttttcagcac tttcaagcac atcacttata ttcttcattg cattaagaga
    44221 aagctctgca aattcatcaa atggaatccc tatatcttct atacttctca ttgcatctct
    44281 atttacattt gaagcaaatc tcgtttcttt cattctttta ataacagact tagtctttac
    44341 actggtaagt tttttatctg gataaactaa agctatagca gttataaacc cagttatagg
    44401 gtctgctgca tatattgctt tttccattag gctagttctt gtaactccca ttccatcatg
    44461 atgaccaagt attgctctat acatttcttc gttaccgaag ttttcttttt taagcagttc
    44521 tactgttgtt gtagcatgtc cttcacatat ttctcctggc tttcttccaa ctatatctga
    44581 atccaaatca tgtaaaagac ctgccactgc ccactcttct tctttttctg gctctagtct
    44641 tttggctaat tctctcataa cagcttcaac agcataacaa tgttttctca ttctatcagt
    44701 ttctaaatac ttaaataaaa tttctaaagc ttcttctctc attttcacca atccccctgt
    44761 tttttcacat atatgatatt tttatatatt aatacacata aattcaagct tttatatata
    44821 ttcatgtata tttttcatgt tatatctatt ctacaccttt atagtagtgt cctgcaactg
    44881 ttccaatttc atctaatttc ttatacaagt ctgtagcctt ttttccaagt ttataattat
    44941 tactcagtac ctcaatgtga gcttcaataa cctttttaac catctcacta tcttcaactg
    45001 taaaatctat cctaaataca tctattccaa tttcctctaa ctcatataga ttgtccaaca
    45061 tacaagtaac cttcgaattg taaattgtac ttctacagaa tatatcttgt gttatcctat
    45121 aacttgcatc cgtaaagtct cttaaagcat aactactttc cttacattta gcaactctct
    45181 tgtccttttt acaatctcta acaactactc ccataggaca gtattctgta accatcatag
    45241 gagcatatcc atatattact gcttcaattt ctacatctgt atattttaaa gtttgtttaa
    45301 tttcatttaa gtttagttct tgtgacaaac aaactgtact tgctccctct tctttgaagt
    45361 gatttatact ttcactattg aatgcattta agtagcaatc tatatttaag tcttttttct
    45421 taaagtattc catagagccc cagttaccaa tttgaacctt attaattcct tttatattat
    45481 ccaatatctc aaattgcttg tactctttat ttctaacaat ccttggtgct gaatagatta
    45541 attttttgtc agtgactgct gtattagagt ggttcttatt caaagtatct attttacttg
    45601 tatttgacaa agtatctatg ttatttgtat ttgaacccat tagttcttta ggagtaccta
    45661 aagacgaaga agtatctaaa tccatagaaa tacttgaatc tatagaaata tttgaatcta
    45721 tagaaatact tgagaccatt tgtaaagctt ttgataatgt atttatatct tcataataga
    45781 ttaaatctac attataattt aatactgctt ctaattgctc caagttctta actttaactc
    45841 taatcttagg agctgtatca agttttttct gtttatttac tctatttggc tggtatttta
    45901 atctattctt cttaaatttt ctatctttta tctttattct ttcttcatct aaaagctcaa
    45961 tacaatccct tctcatttga tttaacacac ttataggcat acttacacct tcatccaggt
    46021 caatttctat ctcttttaac ttatatggtg tatttccaag cttttgaatt tgtgtttcta
    46081 ttttttcttt acttaaagcc acctttatag ctttttcaac tattttgtct ccttctacca
    46141 ccgatttatt tccacaaaaa tcacttataa aaagctctgg ctttttacct agtttaatac
    46201 tcacttttgc acgtatatta gtttttatga actctttatc ttgctgaaat gtagcttgta
    46261 ctctatccat taattcgctg tcagaagtct tatatataag ctgatttctc tttgcttctc
    46321 caacaaaatc taattctatc aaatcccctt tatgtgctat tgtagttatc tctccatcct
    46381 ttattattct tccaatagta cctccaccta agttaattcc atcacctttc ttaagcgtat
    46441 tttctagcat tactctaagt cttttgttct ttctattata atctaaaact tttccaatat
    46501 atagcccttg attattaggt ctattagagt tcattatatc cttaccaact tcacctaata
    46561 tatatccttt tgtaaacttt ctattaaata tggtatacaa attatttatt atttcctcag
    46621 aaatacttat ttttttctta tctacaaatt cattaatcgc ttctctatat cctgaaacca
    46681 cagtagctac atactctggc tttttcattc taccttcaat ttttaaagat aatacacctg
    46741 cttcaattat tttatccaaa tcttctatag tacttaaatc tctaggactc aacaaataat
    46801 ctccattact ttttactact tctcctgtat ttatatctat caactcatac ttttgtctac
    46861 atggctgtgc acacctacct ctatttccag acctattgcc tatcatacta ctcataaggc
    46921 attgacccga gtaacataca cataaagctc catgtacaaa aacttctata tctacagttg
    46981 tattttcaca aatccatttt atctcatcaa catttaattc tcttgctaaa acaactctat
    47041 caaaccctac actctgaagg tattttacat cttctaagga atgtgctacc atttgagtgc
    47101 ttgcatgtag ttcaaagtca ggtaatagtt tttttataag tcttgccatt cctatatcct
    47161 gtactattaa agcatcaata tctatatcgt ataaaaattt tacatactct aaaaaatctt
    47221 ctatttcata ctgttttatc aatgtattta cagtaacaaa gacttgcact ccccttatat
    47281 gagagtattt aacagcctca atcagctcat ttctatcaaa gttatttgca gatgctctag
    47341 cactaaagtc tttaccacct aaatacacag catttgctcc gttttgaact gctgctttta
    47401 atgaatcaaa agagcctact ggtgcaagta attctatttt tttcatattt ttctcctcta
    47461 tttatacttg ttaggttaat ataattatat ctataactta aatatttgta ttaaatttat
    47521 atatttctta ctatatttta aattcttcaa ttaatttata tattagttat ttatatttgg
    47581 tccatttgag ttaacatata cattatttac tatatttagt atatttctat taacttatat
    47641 attgattact gtattttaaa ttcttcaatt aatttatata ctgcttacta aatttaaaac
    47701 attttaatta atttgtacac tgtttactat acttgaaata ctt
//
LOCUS       DAEMIK010000010        44357 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEMIK010000010 DAEMIK010000000
VERSION     DAEMIK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.010"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(317..1186)
                     /gene="gatY"
                     /locus_tag="KPK87_000489"
     CDS             complement(317..1186)
                     /gene="gatY"
                     /locus_tag="KPK87_000489"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG2536684.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(1206..3122)
                     /locus_tag="KPK87_000490"
     CDS             complement(1206..3122)
                     /locus_tag="KPK87_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG2536685.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIKNTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(3125..4045)
                     /locus_tag="KPK87_000491"
     CDS             complement(3125..4045)
                     /locus_tag="KPK87_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG2536686.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGANVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(4147..4899)
                     /locus_tag="KPK87_000492"
     CDS             complement(4147..4899)
                     /locus_tag="KPK87_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2536687.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(5291..6736)
                     /locus_tag="KPK87_000493"
     CDS             complement(5291..6736)
                     /locus_tag="KPK87_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2536688.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDKNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYENVIRTNGEELREK"
     gene            complement(6758..7111)
                     /locus_tag="KPK87_000494"
     CDS             complement(6758..7111)
                     /locus_tag="KPK87_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2536689.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(7140..8387)
                     /locus_tag="KPK87_000495"
                     /pseudo
     CDS             complement(7140..8387)
                     /locus_tag="KPK87_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8395..8712)
                     /locus_tag="KPK87_000496"
     CDS             complement(8395..8712)
                     /locus_tag="KPK87_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2536690.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            8954..10867
                     /locus_tag="KPK87_000497"
     CDS             8954..10867
                     /locus_tag="KPK87_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2536691.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNFQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(10990..12144)
                     /locus_tag="KPK87_000498"
     CDS             complement(10990..12144)
                     /locus_tag="KPK87_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG2536692.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDVTYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(12424..15039)
                     /gene="mngB"
                     /locus_tag="KPK87_000499"
     CDS             complement(12424..15039)
                     /gene="mngB"
                     /locus_tag="KPK87_000499"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG2536693.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEIGHMLEKAYEVCDMVDNDFYTIQINENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKIAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(15168..17108)
                     /gene="mngA"
                     /locus_tag="KPK87_000500"
     CDS             complement(15168..17108)
                     /gene="mngA"
                     /locus_tag="KPK87_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG2536694.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEASFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPENKGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSTSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIHKKIRY"
     gene            complement(17238..17963)
                     /locus_tag="KPK87_000501"
     CDS             complement(17238..17963)
                     /locus_tag="KPK87_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2536695.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(18226..18996)
                     /locus_tag="KPK87_000502"
     CDS             complement(18226..18996)
                     /locus_tag="KPK87_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG2536696.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKIWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(18998..20425)
                     /locus_tag="KPK87_000503"
     CDS             complement(18998..20425)
                     /locus_tag="KPK87_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2536697.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            20816..21538
                     /locus_tag="KPK87_000504"
     CDS             20816..21538
                     /locus_tag="KPK87_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2536698.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            21735..23402
                     /locus_tag="KPK87_000505"
     CDS             21735..23402
                     /locus_tag="KPK87_000505"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG2536699.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMNDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(23498..24817)
                     /locus_tag="KPK87_000506"
     CDS             complement(23498..24817)
                     /locus_tag="KPK87_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2536700.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVDVK"
     gene            complement(24858..25604)
                     /locus_tag="KPK87_000507"
     CDS             complement(24858..25604)
                     /locus_tag="KPK87_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG2536701.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(25848..27857)
                     /locus_tag="KPK87_000508"
     CDS             complement(25848..27857)
                     /locus_tag="KPK87_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2536702.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLRHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSING
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(28295..29728)
                     /locus_tag="KPK87_000509"
     CDS             complement(28295..29728)
                     /locus_tag="KPK87_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2536703.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(29744..31168)
                     /gene="ascB"
                     /locus_tag="KPK87_000510"
     CDS             complement(29744..31168)
                     /gene="ascB"
                     /locus_tag="KPK87_000510"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2536704.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYDRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(31255..33171)
                     /locus_tag="KPK87_000511"
     CDS             complement(31255..33171)
                     /locus_tag="KPK87_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2536705.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQELITVLA"
     gene            complement(33245..34105)
                     /locus_tag="KPK87_000512"
     CDS             complement(33245..34105)
                     /locus_tag="KPK87_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2536706.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(34456..35643)
                     /locus_tag="KPK87_000513"
     CDS             complement(34456..35643)
                     /locus_tag="KPK87_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2536707.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(35657..37048)
                     /locus_tag="KPK87_000514"
     CDS             complement(35657..37048)
                     /locus_tag="KPK87_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG2536708.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(37643..39343)
                     /locus_tag="KPK87_000515"
     CDS             complement(37643..39343)
                     /locus_tag="KPK87_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2536709.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(39315..40718)
                     /locus_tag="KPK87_000516"
     CDS             complement(39315..40718)
                     /locus_tag="KPK87_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2536710.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(40983..41474)
                     /locus_tag="KPK87_000517"
     CDS             complement(40983..41474)
                     /locus_tag="KPK87_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2536711.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEADLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(41500..42312)
                     /locus_tag="KPK87_000518"
     CDS             complement(41500..42312)
                     /locus_tag="KPK87_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2536712.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(42325..43284)
                     /locus_tag="KPK87_000519"
                     /pseudo
     CDS             complement(42325..43284)
                     /locus_tag="KPK87_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(43402..>44357)
                     /locus_tag="KPK87_000520"
     CDS             complement(43402..>44357)
                     /locus_tag="KPK87_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2536713.1"
                     /translation="CSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGF
                     PKFKSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKIRDKQVPQGRILNATISKEP
                     SGRYYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAK
                     LQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQV
                     KNMIKNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEE
                     VKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        14159 a         8171 c         4860 g        17167 t
ORIGIN      
        1 taatatattt aaagttttat aacaatttat agaatatatg tttttatttt agctaaaata
       61 cctctctaaa aatcattttt tacaagtatt tcttatatca acaaatatat tctcattttc
      121 tataaacaac tcatacatat cttcaaactc tagtatacca tatccatgag agtcattatc
      181 tcttaatttt ttaagtgttt ttaataatat cttctttatt ctatatttct aatttgacct
      241 cttaaatata caaactaaag taagatatct actatagaca tcttacttta gcttcattta
      301 tgaataggtt ttattattaa cacttcacta tctctattgc ttttcctaca catccacaaa
      361 cttccatcct actcttaaca aaatctttaa ctgactctaa acctttttta ccatattttt
      421 tagggtcaat tacctctggg tcagaattaa gcacatcctt aaccccattg ctataagcaa
      481 tccttaattc agttgcataa ttaaccttgc aaatccctct ttttatagac tccttaataa
      541 cctcatctgg tattccagac ccaccatgta atactaatgg aactgataca acatctctaa
      601 tttcagataa tctatctaaa tcaagctttg gctctccctt atataatcca tgtgccgtac
      661 ctattgctac tgccaaagat gatacccctg ttctctctac aaactctttt gcttctaatg
      721 ggtctgtata tgctccccca tcaccaccat ctaaatcgtc ttccttacct ccaactttac
      781 ctaattcagc ttcaacaggt atatctgaag gcatacatgc atctacaaca gatttagata
      841 ttgaaatatt atcttcataa ctttcatgag aaccatcaat cataatagaa gtatatccaa
      901 ctctaagtgc ctgcatagct agtccaaagc ttgaaccatg gtctaagtgc attgccactg
      961 gaacacttgc cttctctgat gctaccttta cattcgctaa ataataatct agtcctgcat
     1021 actttattgt tgaaggtgtt gtttgcaata ttactggtga tttaagttct tcagcagcct
     1081 ctattactgc cataaccatc tccatatttt caacattaaa tgcacctact gcataatgcc
     1141 cttcttgtgc atctaataat atttgtttag ttgtcactaa tgccataatt tctcctccta
     1201 tatgtttata ctgatttaat ttcttcgtta tttaattttg atactatttc tctacttgtt
     1261 ttgctattta ataactcatt taaaaattct tcattcatta attttgaaga tagttgagat
     1321 aatgttttta agtgtaaatt atcaccatta tctgtagctg ctatcataaa tataatatgt
     1381 gccaagcttc catcttctga ttcaaagtca aacccttctt ttgatacacc tactgctaca
     1441 cgtggtacct ttactgcatc tgtttttgca tgtggtatcg ctatccccat accaaaacct
     1501 gtagatgata tttcttctct tttaaggatt tcttctttaa acttcttttt atctaaaaga
     1561 acaccagatt tatcaagtat tccaatcatt tcatcaatta catctgtttt attttttccc
     1621 ttaacatcta aaattactgt ttcttcgcta tataaagaaa tattattaga tacattttcc
     1681 gattctgcac tttcattttt atgttcatct tttgttgcat ttgtattttt tatagtattt
     1741 ttctcattta ctactttagg tgtaaccatc atataaagta cagcacctac taatgaacct
     1801 acaaatatac accctaccca taaaagtggc ttattgacta atcctagaac caaaaaccct
     1861 ccatgtggag ctggtacttc tattttcatt atataagtta aaattgatga tattgatgat
     1921 gcaatcataa gtactggcaa cacttttaat ggattttttg ctgcaaaagg tattgccccc
     1981 tctgttatat gagtactacc tagtatataa ttgattaatc ctgctgctct atcttcttct
     2041 gtaaatctat ttttaaataa agtagatgct attgctatta ccaatggtgg tgttatacaa
     2101 gctgcagata cacctgccat aaataaataa tttccctgtc ctagaagcat agttccagtt
     2161 acatatgctg ctttatttac tggacctccc atatcaaatg ctgacataca tcctattact
     2221 attcccaaaa ttatagggct tgaattttgt aaccccgata gaaagttcat catagcattg
     2281 ttaagtgctg aacatggccc accaagtaac aacattgcta cacccacaag acctacgctg
     2341 acaataggaa ttataaaaat cgatttaaga ccttctaatt gtcgtggtaa ttttcttaac
     2401 aaatgtacaa atagtttaac taagtaacct gctaaaaatc ctgatatgat tccacctaaa
     2461 aaccccgaac ctgtatcaaa tgatatcatt cctccaacaa gtcctgccaa cataccagct
     2521 cttcccgata tagattgagc tataaatgct gccaatacag gaaccatcaa gttcatagaa
     2581 tatccaccaa cagattttaa tgtagctgct atttgattat attgttctga atttgggtca
     2641 aatgaataaa tcccccataa aaatgatata gcagtaagta ctccacctgc tactactaaa
     2701 ggtaacatgt gagatacacc attcattaag tctttataca acttttgacc aaacgataaa
     2761 tttccttctt ctgcaatttt actgcttgat aagtttcctt ttctaatagc tacttttcca
     2821 tcaatacatc tttgtataag ttcattagct ttatgtatac cttctttgac aggtacttct
     2881 attactggct ttccattgaa tctgtccata tcaacatttt tatcacaagc aactattatt
     2941 gcatctgcca tttcgatatc ttttgctgtt aacttatttt ctacacctat agctccattc
     3001 gtttcaactt ttatgtcaca ccctaattcc ctggctgaat tttttaatgc ttcttctgcc
     3061 ataaaagtat gagctattcc agttggacaa cctgttaccc ctagaatcat tttctttccc
     3121 atttttatac cacctttctg actcttatat tttttatata atcttccaca ttctccaaat
     3181 tacctatacc attactttca gccacatttg ctcctgttgc tgctgacctt tttaatgctt
     3241 cttcaatatg ttctggattt tccaaccata tacttaaaaa agcagctaat gcagaatcac
     3301 cagcacatgc agagctgact actgtaactg gttgagcact agcataataa attgattttc
     3361 cattataaaa ataagaacct ttttctccta aagttagcaa tatattttga gcacctttta
     3421 aatgcagtaa tttaagtaca tccttaatat cttcttcatc tcttacaata attccaaata
     3481 tatctttgat ttcttcatca tttggtttta tcaaatatgg attatactgt agtaactcct
     3541 ttaattttgg tgaacttata tcgagtattg tctttatact tttattttta caaattttaa
     3601 atatatcctc atagtaatta tcatcaatac caggaggtag acttccactt attgataagt
     3661 aacttatatc tgctaatgac tgtatcttat ctagcatatc tagtttttgt ttttcattta
     3721 catatgaccc agagtttaca aatttaaatt catcacttcc atcatttaaa aatatattta
     3781 tacgagttgt atcttctacc caagtaggca atacgtcaaa tcctctcttt tcgctttctt
     3841 caactatata ttttcctgaa aatcctccaa aaaatcctaa tatcttagat tctataccat
     3901 aatgacctag tacaaaagtt acatttaatc cctttccatt aggagaatat acagcatttg
     3961 atgtacgatt tactaatttt ctctttatcc cattagtaga tatattcata tctattgcag
     4021 gattcgtagt taatgtatat atcattgtct tcccccttgc tcgtgctgtg tatgttttca
     4081 ttataataaa aaagtctatg aaaatctttt cacaaataga aaatcgattt tcatagactt
     4141 ttcaatttaa ttttcagaat tattatatct gaccatataa tctataatta tcctagatat
     4201 aatatacaag ggcaatctgg aagatacttc atattcagaa attgatatat gttcatgttt
     4261 gtatcctaca atatataaat ccgaatactt tataagtttt gcactttcac tacaacagca
     4321 agttataacc ttagctccaa ctaaattggc attctgtgct gattctacaa gctctatggt
     4381 atttccattc aatgaaaata taattataag ttcgtctttt tttattgatt tagattttat
     4441 tttcattatg ttggggtcat ttataaaaac aacattaaaa tcaagtaatt gcaatttaag
     4501 actaaactct tttgctacat attcagtaag ccctcttgcc aaaatatata tcttactaga
     4561 tttatttata gtttttatta tattgattag tgtagttaaa gatatttgtt ctataactct
     4621 ctgtgcttct ataaaggatt tatttattat atcccccata ttttgtatat catcattttc
     4681 atcttttact gttaatctat acctaagttc attgaatcca tttaaaccac attttctaat
     4741 tgctcttgaa acggttgctg gagatgaata tgtttcatga gcaatctcta ctatagataa
     4801 ttctggtaat attgattcat tttcatttat aaatttaatt atttcaagtt ccgtcttcgt
     4861 tagattttca gaattacttt tgtttagctc aattatcata tgttatctcc ccttatataa
     4921 gttttatttg tctaaattta tcgagtaata taatatatta cctcttaact acttattttt
     4981 atatacaatt aagatttagt caatgtctat atatgtaaat ttataatata ataatttttt
     5041 gcttaaagtt tattatgtta tcttatagca ttttaaaata aaaaagaatt gtacctctac
     5101 caaaatttgg atgaagtaca atagatattc ctcatactga tatgtttatg attaaaagta
     5161 taaatataaa tttcgctatg aatcatataa ttaataaatc attttattta caattataac
     5221 cccccttata aaactatatt aataatatat acagtattta taaggggggt tgtttatatt
     5281 taaaaactat ttacttttct cttaattctt ctccattagt tcttattaca ttttcatacc
     5341 aataaaaact atctttttta taacgttctt tacttccatt tccattgtta tctatatcca
     5401 catatataaa tccatatctt ttttccattt cttgagatga acaacttact atatctattg
     5461 gtgaccacat acaataagct aatatttcgg ttccatcagc tatagcttca cctatttgtt
     5521 ctatatgttt tcctaaataa tctattctgt aactatcatg aacttttcca tcttcaaatt
     5581 tatcatgcat tccaaatcca ttttcagcta tcattattgg cttttggtat ctatcataat
     5641 attgagataa agtattgtat aatccttttg ggtcaattgc ccacccccaa ggatttgctt
     5701 ttaaatatgg attctttata ttgtcagttg gattcatggt gtttttactt gctgatactg
     5761 ttgtagaata atagtatgat aaagctagaa aatccatagt attttctttt aagattttat
     5821 catcaccttc ctccataact atatttatgt tattttcttt gaaaaatctc ttaatataga
     5881 ctggatactc tcccctaaaa cttacatcag tgaaaaaata ttgtagccta tttcttttta
     5941 aagcaaatac tacattttca gggtcacaat cttctggata agatgtacaa tctgccatca
     6001 tcatccctat catcatatct gaatttattt cttttccaag cttcttagtc aaagctgtag
     6061 ctatgaattg atgatgaata gcttgatatc tagcctcttc tacattatct acttggtcta
     6121 cacaaaatgc aactgaatta aatgattcat aatgtattaa atttatttga tttataacta
     6181 tccaatattt aactttattt ttatatctat ttagtattaa ttctgcatat ctaacaaaga
     6241 aatctaccac ttttctatta gaccatcctt tatattttaa tactaaattc aaaggagtct
     6301 catagtgaag tagtgttaca ataggttcca taccattatc tattacttca tttataagat
     6361 tatcataaaa ctttaatccc tcttcattag gctctaaatc atctccattt ggaaatattc
     6421 tagcccaatt tattgaaaat ctaaaacact ttaaccctag ctctttcaaa agctttaaat
     6481 cttctttgta tgtatgataa aagtctattc cactacgctt aggatagtac ccatttttat
     6541 ctttcatagc aaactttatt ctttctatag tcatatcaga ctcataaggt ttagatatat
     6601 ctctatcttt atcatacata tgtacatcag ctaaacacca acctttgcca ccttcattcc
     6661 atgcaccttc taattgatta gctgctacag ctcctcccca taaaaaacct ttaggaaact
     6721 tacccaattt aatcactcct tattttattt atttaaatca tttactatta tttaccttat
     6781 attctataga ctttactata cctactaaag acatagttat atttaactcg ctgaatatgg
     6841 tcataagagt atcttgagca tgtgcaaata acagcgaatg tgctcttttc tctccaccag
     6901 cttccccttg tataatcttg gtttgagctc tatgtgcttc tgtcataaac ccttttgctt
     6961 tagctagttt taaatcggca tttctataat caaacttttc caaatcatta agagcatgtt
     7021 taatttctgt tctagcctca cctgcacgaa gtattatttg cattgctact tctactaatt
     7081 cttcatctat tatatgttct tcaattgtca tatttataat ttcctttcaa tttctaaatt
     7141 taaacctctt ggctctctaa ttttatctct tctttctcat aagctttaaa aaatggatac
     7201 catatcataa atagcaatac tatatttatt gctaaaacaa taagccctct aacatcttga
     7261 tttaccataa aagtagatat accaaaagga acatactata aattaaacac cttacttgga
     7321 attgtcacaa atcccatact cattgataag taagtagtta ttgatattac tactgttcct
     7381 atccacatag gtaacattaa aaatgggttc catgccactg gagctccaaa tattactggt
     7441 tcatttatat tcattataga agggacaata catgctcttc ctattgcttt tattcttcca
     7501 actctggata aacacatcat tataactaat ggtaatgtag cacccaaccc tcctaaagtt
     7561 atccaacctg tttgaataac ttcgtaaaca tatatattag ttgctactcc tcctgatgct
     7621 actgctgctg cattagctgc aattgcacca agttgaattg aataagttat aggagatagt
     7681 acccacccac ttattcctaa agaatatata aatactacta agaagtttaa gagtacaaat
     7741 ccaggtaatg tctgtgctat atctgatact ggtttaaata ctctaactat tatttcaaac
     7801 atgtcaaagt tgagtccata aattaatccc cagcctacaa acattatcac tgttataggt
     7861 aacatattat caaaccactt gcttacaaaa tctggaagtg ctgaatcttc tccgaaaaat
     7921 gaaaaagaac caaataaaga cattattaat ccagagaata atcctgcaac tagcgataca
     7981 agcattcctt ctcctccaaa cctgttgaag ttaaatatag ctccttcatt cgtaatctct
     8041 ggacgaatca tcattaacat taatccagcg cctgcaagac cagctactac actcatactt
     8101 tcaaatttct tattcttcat tatttccata ggcaataaaa atgctataaa taatcctaaa
     8161 agtccaaatg tatattgatt tattggactt aaatcaggta aaaagcttat gtaattcttc
     8221 aagacagaaa ctatagttac taatgagcct acaaatacca ttggcaaaac tttcattata
     8281 gttgattgaa ctgattgtac ccatatattc ttagtcatat tatttacaac aggagtaaat
     8341 ttttcactta aaaaatctat aaatttattc ataaacactc tccccataaa tattttattt
     8401 gtttgatatt agttctagtg cttcttccaa agcctttgca ccatctagtc ttccatatgc
     8461 agttttagat atacatgcta ctggaacgtt atattcatta gccttttgtt gaatttcttc
     8521 cttcatataa tctaaatgtg gtcctagtaa taaacaatct atttctgata aatacttatc
     8581 tatctctgac tcacttctcg cttttatatc tgcttctata ccttttttct ttgcactctt
     8641 tctcatacta gttgccataa aacctgtact tgctcctgaa ccacacgcca aaagcactct
     8701 tatatttttc ataataatac ctccaatatt tttatccatt aatttatttc ttaatgaact
     8761 ttttatttta ttatgtttat attgtactta ccaaataagt tcatttcaca taaatatatt
     8821 tcctaataat aggaaatcag ttactcttaa ttaaacatat taatatagta taatgtatct
     8881 tataatacgt acattcaatt atataattat catatcatcc agattttatt ttaaaactat
     8941 gcgagggaat ctaatgaaaa catctaaaaa agaactattt ttaaatttca taaaaacaca
     9001 acatgattgg atagattcat ctacattggc taattatctt aatgtctcaa cgcgttcaat
     9061 tagaaaatat gtaaacgaaa ttaattccaa tggtgaattt atattatcct ctaaaaaagg
     9121 atataaagta aatctcaata ataattttca aaccaaggtt gatagttcag aaaatatatc
     9181 acctgataac aggttaaatt taattttaaa agaacttata gttaatagta atggaattaa
     9241 tatatttgat ttaagtgaag aattgtttgt tagcccagct actattgaag gggatatagt
     9301 caaagctaat aaatttatag gaagttataa tttaaagata aagcaatcga aatttttatt
     9361 gaaactaatt ggaaatgaat ctgcaaagcg caagttaatg agctctataa tatttaaaga
     9421 aacgggaagt gattttttaa gcttatttga tgtacaaaaa atatatcaag agtataattt
     9481 gactaaacta aaagaaaata taatatatat attaaaaaaa tacaatttat ttataaatga
     9541 gtatgctata aacaacatac ttcttcattt aatgataact atagacagaa taaaaaagaa
     9601 taattacata gactctgttg aagtagtaaa ctacatagac aataacgtag atataaatat
     9661 tgcaaaagac atatcaaatt ttttagaatc tgaatacaat ataactttaa ctagtgcgga
     9721 attatactat ttagtatttc agttaacaaa caaaactaca gtcttaaact acaatcaaat
     9781 ggatactaaa tcattgtcaa attatataga tgaacacttt gttaaattaa ctaaaaaaat
     9841 aattaaaaat gtatatgact tatatcttat agatttatct gatgaggaat ttgttgtaaa
     9901 atttactctt catgtaaaaa atttaatcag tagagccaaa aataatcaag ttttaagaaa
     9961 tcaaatacct caaaagttaa aagattccta ccctttgata tatgatatat ctgtttatat
    10021 ttgcaatcag attcaaactt tagaaaatgt agatatagac gaagatgaaa tatcttatat
    10081 atctcttcat gtaggttcat tctttgatag acaaaaatta ttagaagata aagttctatg
    10141 tgcattaatt actccaaatt actatgattt acaattcaaa atagtaagag accttgagaa
    10201 gagatttaat gaatccatag aaattattca gattttttca gatactcata atcttgattt
    10261 tgacaataag gtagacatgg ttattactac tttaccaatt aacgatagat gccctatacc
    10321 ttttgtttat gtaaatcctt acctaaatag aaaggattat gataatattc aaagtaaatt
    10381 tattcagatt aaagatagaa aaaatatact tactgtacaa aatcatttag aaatgtactt
    10441 tagtgaatct ttgtttatga aaaatatata tttagatagt gctaaagatt atataaaatt
    10501 tatgggaaat attctgtacg aaaataaata tgtcaaaccc aattatatag atgatgttct
    10561 tataagagaa aaaatgtctt caactgcatt taataacaat gtagcaattc ctcatagcat
    10621 gaaaatggat gctttaaaaa ctggagtatg tttaattgtt aacgataaac cagttaaatg
    10681 gggtgaggaa aaagttcaaa ttatagctat gatacctata aatgaaaaag aaaaagaaaa
    10741 gttcaattac atatttgaaa gctttattga aatattatct gagtggaata atgttaaaga
    10801 acttactaag gcagataact atagtagttt tatgaataga atagcttatc ttattcaaaa
    10861 tatctaaatc cgttaattta tttccaatat atgtttctta agttattaac ataaaataga
    10921 taagctgtca cactaaaaag tctaaattaa tatgtgacag ctttttacct aatatacctt
    10981 gttttaatac tacatatttt ttgactgttg tatactagtt ataagattga atatttgtgt
    11041 tgttgtagct gtcatattct cactagcagt tgctttatcc attgcttctt ctattgtcat
    11101 agctctattt actatagaaa aataagcatc tataccctct tcattacatt taactgcatc
    11161 ttctgttgtg gctccagcca aacctattac ttttacccca tatttttttg ccagttttgc
    11221 aactcctatt ggagccttgc ccatagcagt ttgattgtcc aatctacctt caccagttat
    11281 gacaaaatct gcatctttta actcttcctc taatttaatt tcttctaata ttatttttat
    11341 tccagattct aattttgaat ttaaaaatgc taaaaaagca aaacctaatc ctcctgctgc
    11401 tcctgctcct tctacattag ctacatcttt acttaagtaa tttttaacta ctttggaaaa
    11461 atttctaagc ccattatcta actcctcaat tatttctgat gttgcacctt tttgagaccc
    11521 gtatatgtat gcagctccat ttttcccaaa cagcggatta tttacatcac aggctatttt
    11581 aaacttacat tcatctaatt cctttaatct attttctatt tttatatgtc ttatttcatt
    11641 taaaactttt ccacctaatc ctactaactt gttattttta tcataaaact caaaccctaa
    11701 ggcttgaagc atacctatcc cagcatcatt agtagcactt ccacctatcc caactataaa
    11761 atttctatag cctttattta ttgcttcctt tattatttcc ccaacaccat aagttgtagt
    11821 gtataaagga tttctaagtt cagtcggaac caatgttaat cctgatgctt gagctatttc
    11881 tattattgct gtacttgtat tttttaataa tccatatgtt acatccactt ttttagctat
    11941 tggtccagta acttctatag tctctttttt accattcatc ccttgaacta aagcatctac
    12001 tgtcccttca ccaccatcag caagtggcaa tatctttact tgcgcatctt ctttaacctt
    12061 caatatcccc ttttttattg catttcctgc ctctattgaa gataagcttc ctttcaaaga
    12121 atctatagaa attaatattt tcatatttat ctactccttt ttattactcc ttattttaaa
    12181 catataattt tataagctta atttatcttt tattttatac tctacatctc tctttgtata
    12241 caacttatac cattaatatt cttttatctt ataaaaaggg agtgtctcaa aataaatttc
    12301 ttagttcatg agacactctt tttgtataaa atcaaatata tgtttattat ttcaacaatg
    12361 ataaggagga ttatctctat tttaagataa ttccttttta gcactatcta atcattttta
    12421 gcactattta accaatatag ttttaacttg gttcattaag cactctacat ttacagagtt
    12481 attatctatt gatatagatt ctaattcttt ttcatttagg ttaatccatt tagctatttt
    12541 aatatttttg ttaaatacta tgttagctga ttttgtttct tctttagaat tataaaatct
    12601 aactactaat gcctcttgtt tttcagcctt ttttacaaca cttaacacaa ggtccttatt
    12661 agtttcctta agtaaactat atgaataatc tacttctatt tctgattgat tcaatttcat
    12721 agcattatat ggcattttat tataagtttg tatttttgtt aaatactctt tagccatatt
    12781 tgcaacattt gcttctagtg tagatttttc atgagtaact attgctaagt ctatttttat
    12841 atctcctatc atttgagaat ctggagtagg caactttatt cctgatggac gacctgggcg
    12901 tcttgccatt tcttctttac ctaggaatcc tacacttcta aataatgtta tagctattgt
    12961 gtcaaatttt tcccctacta tttcaaactc tcttgtactg tttgtaagaa cagctactcc
    13021 atgctctttg tcagacaatc caacgaatgt tagcattgga tatatagaat ctggtctttc
    13081 actccattct tcttgttgcc aaacttccat agcatcatca tacacacttc tctttatatg
    13141 tccaaactga ttatcagaca cagaaaactt tgaagctata tttgttggta tataagttct
    13201 aaccctacaa tctcttgctt ggttatttat gttgaatact acatctatta tagctttatc
    13261 atttggaaca tttataacta tatcaaagtc aataaatgaa tctgccctat tattttttct
    13321 agcttgtata tcttttggta cttttaatct ataatttatc tttatctcat tattaaatct
    13381 atttttcttt atagtggaat ttgctactat attatcgtta aatattaatt tctcatttgg
    13441 aagtggtgaa aaatcatact catctccttc atctcctcca ttttccatca ataatacatt
    13501 ttcaaaagtt ttatttaatt ttttatcaaa tatctttaat gtcccgtttt cattaatttg
    13561 aatagtataa aagtcattat ctaccatgtc acatacttca taagcttttt ctaacatatg
    13621 tccaatttct tttatcatat acgccttata ccccattgca ggtattttat ctttaaagtt
    13681 tattgtatat accataaatg aatcatagtt tccataatga acaatttgac ggtctattaa
    13741 tcctgcatct attacttctt tatgtaccac ttcaaaatcc aacttattat tatctttatc
    13801 aaatatttca aatgctttca ttttagttat aacttgggcg ttcactattt cattcctatc
    13861 gtaaggcatt aagttaaatg ctactaactt atctaaagcc atttcacaac tcattgcatc
    13921 aactattctt cttttgtaaa atgttattaa ttggtctact ttttcctcag ctaagaaaaa
    13981 tctattcatt atttcatggt gaactttatc tgaacaacaa catccaatgc tgtcatgagc
    14041 atgatttttc attatttctt tccaaattaa ctctattaat ccatgatgat attcaaatcc
    14101 tagagaatat gcaatagaag ctagtggctc caatatgttg gttatctttg attctattct
    14161 agcatttgct gattttatat ccattcttgt tgagaatata cttctatgaa ccctcatata
    14221 tttcccatct aaaaattccc cttttattgt atctatatcc tcattttttt ctagctcttt
    14281 aaatatgttt tcatatctac ttaagaaaaa ttttctctct ggataacatt cttttaattt
    14341 atatataact tcaaatatat tcttttgaat tggcatctgg tcatgtccat ttggaagtat
    14401 tatatggtca gtagttgcac ccttatcaag aaccggtaaa tatttatcca ttcttgttct
    14461 tagctcatct tcattcattg gtagatattt tcctattgca taacctaatg gaagtatttg
    14521 aaccaaaaca gaagacccat catcactctt ccaattaaac tctgttttat tagttccttt
    14581 tctttcagaa catcctctcc aaaacattga gtattttata tcaaacccat ttagtatttg
    14641 tggcatttga gcactttgac caaatgaatc tggtaaatat cctattttca tatattctcc
    14701 aaattcatca caatctttta ttccatatag taaatttcta agtatggatt ctccaccaac
    14761 taccatttca tcagtttgag tataccaagg acctattatt aaccttcctt cttgaactaa
    14821 tttttttatt ctctccttac attcaggctt aactgctaaa taatcttcta atatagcagt
    14881 ttgaccatcc atcacataat atggatagtt atgattattt tctaacatat ccattatttc
    14941 ttccatatta tttacaagta atattctaga ttcctcagtt gagaagtacc attctctatc
    15001 ccaatgcata tgaggaacta tgtgtacatt ttttttcatt gtttatcctc caaaacttat
    15061 ctgataatat tttctgtcta attaaaaatc caataaaaat actactgagt ttacaaaatt
    15121 ttttaatatt tattttatca ctataattaa acctttgatt taaatatcta ataacgtatc
    15181 tttttatgta tctttctttg atttttttgt ttcctagtta ttattaataa tattgctgat
    15241 attatagctc caagtactgc cgcaaagaac catattgatg ctataataaa tcctgacgta
    15301 cttccctcta aaagcgccaa tgagaatatc cccgctccag gcacttgaag acctatattg
    15361 aagaatgcta ctatagaccc agttatggct gaaccagcta ccaatgatgg tataacacgg
    15421 attgggtcct ctatcataaa tggtattgca ccttcagtta ttccagccaa tcctaatatc
    15481 caagtttgct ttcctatttc cttttctgat tctgaaaata aatctctgcc taaaacagta
    15541 gctattgtaa ctccaaaagc tgaaaccatc ttaactgatg caaatattgc ataaggtata
    15601 aaatttccac ttgctattgc tcctattgca aatgtatagg ctgctttatt tattggtcca
    15661 cctaagtcaa atgaaaccat agctcctatt attgcaccta gtagtattgc atttactcca
    15721 gtcatcccat ttaaccaatt tataagacca ttgttaagtg ctgctacagg ctttcctact
    15781 acaaataaca tcaataaacc tactaccaaa gaaccaacta ctggatatac ccaaaaactt
    15841 acgaatccag ctaatgtgcc tttggctggt atattcttct ttaaaaattt tactatataa
    15901 ccagcaagta aacctcctaa catacctcca agaaatccac tacctattaa atcagctgct
    15961 agacctgctg caaatcctgg tccaagacct ggtttttcag ctagtgagta agacatatat
    16021 gctgataata ctggaaccat caatgtacct aacatacctc cagaaagctt tcttaattgc
    16081 cataaccaag aaccttcttg gttgtatagt tcttgtagac caaatgcttg agatgctaat
    16141 actgcaaatg caagtatcat acctcctgcc acaataagtg gtattatatg agatactcct
    16201 gtaagtattg ctgattttac atcttttttc catgatgatt tttcacaata tgatatatca
    16261 gatgattttt ctatataatc acctttacct tgtttttgtg ctacttctaa agcttcgtat
    16321 attatttctt ttgctctttt tagtggagct gctactgacg tttttatgtt tggcaaatgt
    16381 tcaaatcttt ctgtattttt aggtgctaca tctgttgcat atattattgc atctgccctt
    16441 tttattatat ctggtgtatg agggtccacc ataccattag ctccttgctt ttctacatac
    16501 acatccacac cgatttcttt accagcttta attaatgctt ctgctgacat atatgtatgt
    16561 gctatacctg cagggcatgc tgttattgct aatacaattt tttttgattc attttgaatc
    16621 ctaatctctt cttttatttg ttctttcttt tcttttatat ctaagttatt aaataactct
    16681 ttcttagttt ttgaatttaa caacatattc atataatctt catttgacag tctagtaact
    16741 aactcagaaa gtatttgtat gtgtgtattt ccacctttat tttctggtac tgctattaaa
    16801 aatattagct ctactttatt atttgggtct acgctttccc aattttctat tggtgtctct
    16861 aaggtcatag ctgcaaatga agcttcttta actgacttag attttccatg aggaattgca
    16921 agccctcttt caaaacctgt tggagataaa gtttctctgt acattacagc ttctaaatat
    16981 tcctcataag atgttaactt gccttcatca tatagctttc ttgctaagaa ctttattgcg
    17041 tctttctttg tttttatttt ttgttttaaa aaaacaaggt tttcatttgt gattttctta
    17101 aattccaaaa ttacgccctc cttaaataat ttaatgctat gaatatagca taaaataaat
    17161 tatgaaaatg tttttttgaa attatacaca caatttcttc ttaaaaaata aagatttgtg
    17221 tatttataca caaatctcta tcttaaccag tattgctcta ttattgctct cataatcatc
    17281 ataattggaa ccttatctga tatattgtac ccatttactt gtttaattgt tgaatagcaa
    17341 tataaactta tatctgcaat ttgttgcaat tgattttcac atatatgtgt tagagctact
    17401 accttacaac ccttattaac tgctgtcttt gctgcttcta taacttcttt cgtttctcca
    17461 cttaaactaa gtgcaaaaaa catatcttgt tcacctaatt ttcctatatc atgaagtact
    17521 tcatgtcttt gactatagta agttatatta tcatgcccac ctctaatttc ttttactaat
    17581 atctcacata aaggaatgtt ttgacctatt ccataaacta taatactcct agatttttga
    17641 attatatctg ctactttatt tattttttca taatctatta gctctaatgt cttttttata
    17701 tctgattcaa aaccattttt tggctcttta tcctcatttt ttaactcttc ttttaaatta
    17761 ttttttagtt gagagaaacc atcatactcc atttttttac atagcctaac tatggtattt
    17821 gggacagtaa aaaatttatc tgctaaagat tgtattgata attttactat ttcttctttg
    17881 ttttttctca tatattcaat tatcatgtct tctgtatctg taaatttata ctcatatttt
    17941 tttgctcttt cataaaattc catagtttct cctccatcta ttttattatt cataagtaaa
    18001 attcaaaaat attagctaaa attttcgtta atataattat aatttaatat ttttaattta
    18061 ttaatgcata ctactctttt tattatacat aaaaaatgtg tacttcctat attgatagag
    18121 attattcaag tttttatttt gatacatctc tatcaatatt tttttagaaa atacacattt
    18181 ttttcaaata cttattttta attaaaactt taggtaatct attctttata tataattatt
    18241 agacttgaaa atatctttaa aatcaaccaa ttcaatatta tttcttttta accatatttt
    18301 aacttcctta tcacatagca ttgcatgttc atatactctt tcttgaataa gagaagatga
    18361 ttttataaga ctataatcta taaatcctgg atgagtaacc atcaatgtta tatctttatc
    18421 taaatttata tatttttctt caaataattt aacaaaagat ttatttaact tgtaaaaatc
    18481 attctgagga tatttacttg gcatgctaat acatgaatct aaataatagg gacaacttgg
    18541 tataccgtat tcttttgcag catcacacat tgctgatatt gatacatctt cttctatacc
    18601 atgtatatca atatactcag gcaatttact catatacaac ttgtaagttt caatttgagc
    18661 acaaacttct tcatatactt cttcataatt aaaaacctct ttacctgtca gtagttgcgt
    18721 tcttcttatt ttgctagaaa caaattttcc accttcatct aacaaattct ttatcttaat
    18781 atgttctttg caacaaggtt ctcctattac taaattaaca tgtaaaccaa aacatacatc
    18841 atcatttttt accatttcta tagcttgtct tgaaaaaggc atattcacca tgattccagc
    18901 acttgtaaca ataccgttct tataagaaga tattatccct agtgtaacac cttctgaata
    18961 tcctatatca tctgccctaa ctactagttt cttcatttta tgcgtctgcc tgctttctca
    19021 actcatcaag ttgcatttcc atctcatcct ttactttaac aacatccgtt cctatgatga
    19081 tttgaactga tgtattactc aatttaatta cccctttggc tcctgttttt ttaatattat
    19141 catcattcaa aatagtagca tccttcactt ctaaacgtaa tctcgtcata caattattaa
    19201 gagtcactat attttcataa ccaccacaat ttttaattat ctgtttagcc atatatgaaa
    19261 aattaccatg agaaagtttt aaattctttt ctttttcact aactttatca cctatctcta
    19321 tctcaacttc acgtcctggt gtttgcacat tatcttttat tattatatat ttaaatgtta
    19381 cataatataa tacaaagaac acaactccta caggtataac taaccacgct ttatctccat
    19441 aactaaaact taagacatag tcaattatac ttgctccatt aaatgaaccc aagcgagcac
    19501 ccagtaaata tagtacaact ccacctagac cagaataaaa agaatgaatt atatataact
    19561 ttggagatgc aaataaaaac gaaaattcta ttggttcagt tactgttgcc ataaaagaag
    19621 tcaatgctcc agcggtcatt gcacctttta cttcttgctt ctttttatct tgtgctgttt
    19681 tatatatagc ataagaaata gcaggtattg aaaacatcat aattatgtaa aatcctgtcc
    19741 agaagaatcc tgcatttggg tctcctgcca taaatctagg aatttctcct cttataactt
    19801 ctcctgttga tgttgtatat tcacccattt caaataatat gtaagcattt aaaacttgat
    19861 gtaacccagt tggtattagt gcacggttac agaataaaaa caagaatatt cctattgcac
    19921 cagctcctcc caaccaaaaa ccaaattgat tcattgcagc ttgtggatat atccataata
    19981 aactaaatat atatgataaa attaaagcaa ccactggtga tagtgtaatt acacattttt
    20041 ctcctgcaaa gaagcttaaa aatgctggaa ctttaacttt atatgtttta ttgtaagtcc
    20101 atgctataca agctccaaca attatcccac caaatacacc tgcatttaat gtatcattca
    20161 ataactctaa atttgactta aatatcatat atgcaactac ggctgcaacc attacacttg
    20221 tcttgtcttt agactttgag taggcactag ctgctcctaa taaaaataag tagtccacat
    20281 ttgaaaatac tatattagat atattagata tagctggcac attgagcata tttggaccaa
    20341 ataataatcc taatatacct gctgctggca ttacagctat tggcaccagc atagtctttc
    20401 ctaaaagtga cagttgttgc atcattttgt ttaagaacct cttcatatac aatccccttt
    20461 ctatttcggt ttaatttttt gtgaaaacga tttattcaca ttttatattt taaatattat
    20521 atttgtttaa acaaattgtc aatatttttt attcatttta tttttatcat ttagtttaac
    20581 atataaatcc ttttaagtgt attgctatat ctagcctatt tttttaattc atttttttta
    20641 ttttaaaaat atgtttaacc atatatttga aacaacccac aaaataattc tattttgtcc
    20701 tttttatggt tgatatgatt ctattttcac tatacatgtc taaacacatt tgatataatt
    20761 tgttcatatc agttcaataa tattactaaa gacctttaag gagggatagg ggtatatggc
    20821 taaaaataaa ttttcagaga tatatgaagt attgaaagag gaaatacttg atggtaaata
    20881 tacaagtaat atgatgctgc ctactgaact tcaacttatt gaaaggtttt catgtagcag
    20941 aaacactgta cgtagagcaa tttctcaatt aaacacagaa ggatatgttc aaagtataaa
    21001 aggcaagggt gttgtagtgt tagaaaatag ttgttctaat gatttttttc taaatatgca
    21061 caatttcaaa ggtgtagaat cgattgtaga agataaaaag gttaatactg ctactagtgt
    21121 tctccacttc agcaaaatct taattgataa taagttatct aaaaagacag gttttaaagt
    21181 tggtagtgaa gtatattacc ttcacagact aagatatatt gataacatac ctaaaattct
    21241 cgacattaac tattttctat gtagtatagt taaagacctt gatgtttcta tagcacaagg
    21301 ctctatttat aaatatatag aagagtgtct aggtacaaaa attgtttcat ccagaaagat
    21361 atttaaaatt gaaaaagcta cagagcttga gttaaaaacg ctacctctaa atgattataa
    21421 ttgtgttggt gttattaaaa atagtgtata tactgatgat ggaaagcttt ttgaatatac
    21481 tgaatcaaag catacacctg aaacatttgt attcatggat gttactcaaa gatattaaaa
    21541 aaattatgtt taattaaaca taattttttt acgtttagac actttatccc atactaacct
    21601 cactattcta tatacaatta tatgtttata caaataatta tttattgtat attttagctt
    21661 taatattagt ttaacgcttg acaatttgtt taaacatatt ataattaact tataaattac
    21721 aggaggtatc aattatgaaa aattggtgga aaaaagcaac tgtctatcaa atatatccaa
    21781 aaagtttcaa agatagcaac aatgatggta taggtgatat aaatggtatt atagaaaaat
    21841 tagattatct gtattcttta ggtgttgatt tattatggtt gactcctatg tacgtttctc
    21901 ctcaaagaga taatggatat gatatagaag attattataa tatagaccca aaatatggga
    21961 caatgaatga ctttgaaaaa ctcttaaaag aagctcataa aagagacata aaaataatga
    22021 tggatatggt cctaaaccat acatctactg aacataaatg gtttaaagaa tctaaaaaaa
    22081 gcaaagacaa tccatatcgt gattattatt tctggaaaga tgctaaacct gatggttctg
    22141 ttcctaataa ctggatatcc agatttagtg gaactgcttg gaaatatgat gaaaccacta
    22201 accaatatta tttacattta tttgaagaaa ctcaagctga tttaaattgg gaaaatgaaa
    22261 aggttcgtga agaatgttac aaaatactag aattttgggc tgataaaggt attgatggtt
    22321 ttcgtttaga tgtagtcaac ctactctcca aaacacctgg attacctgat gaccctataa
    22381 caggaccaaa aggagatgga agaactcact acgctgatgg tcctcgtata cacgaatatt
    22441 tacataatat gaatcaaaaa gttttcaaac ctaaaaatat agttactgta ggtgaaatgt
    22501 catctactac acctgaagag tgtatcaact atactagaga aaatcgagaa gaactatcta
    22561 tggtttttag cttccatcat atgaaaacag attataaagg aggcgcaaaa tggactaatg
    22621 aaatgttctc tctggataaa ttaaaaaaag cacaatctga ctttcaatat aaaatgtatg
    22681 aaggaaaggg atggaatgct ctattttact ctaatcacga ccaacctaga gctttatctc
    22741 gatttggaga tgatagattt tttcgtcaag aaagtgctaa gatgttagcc ataacattat
    22801 ttggtctaca gggtactaca tacatatatc aaggtgaaga aataggtatg acaaatgcct
    22861 actttactaa aatagatgaa tatgatgatg ctgaaagtaa gaatgcatac tatcatatga
    22921 ttaaatctgg tattgatgaa aaggaggcat tgcttatact tcaacaaaaa tccagagata
    22981 atgcccgtac tcctatgcaa tgggataata ctatttataa ggggttttct aatcataaac
    23041 cgtggttaaa ggttaataat gacaatatta gtgtagaaaa tgagcttaat gattctcgtt
    23101 cagtatttta tacttatcaa agattaatca aatatcgtaa acaatatgat atttttacag
    23161 aaggtactta tagattgtta gatgaaaatc ataaaaatct atttgtttat gaaagagagt
    23221 ataaaaacca aaatcttctt gttataagta attttactca tgataatgta gtatataaat
    23281 tacctgaaac ttatttgaat aaattaaact acacaatact tgaaaccaat tacactagag
    23341 atataattga aaataaaatg gaaataaaac cttacgaaag tataatgata tattataaat
    23401 aaagttattt atgtaaaaat cggtataaca ccctggtgtt ataccaattt tactaaattc
    23461 aaagtaatat ttatagaata tctatagatt agaataatta tttgacgtct acatcattat
    23521 atttctttga atatacttta gtcatataga aatatatagg aactacaatt atcatcccac
    23581 ctaatcccca aactatcttc ataggttcat caatacctgc ttttatcagt aataatatac
    23641 ttataataac tgcaaatatt ggtataattg ctcctccagg tattctaaaa tttacatctt
    23701 tatcagcata cttctttctc aatattagta cagctaaaca tgtaggaata tattgaataa
    23761 atctagatac aacacttatt tgagataatg ttgtgaatga acccgaccat gctattagta
    23821 aagttcctat gacagatact ataattgcta tataaggagc tccattttta ttagtttttc
    23881 ttatagactt aggcattaat ccttgttcaa ccaaagcagc agcagacctt ggtgtaaata
    23941 tggatgatgc aatatttata cctccaacag atactatagt tcctattgta attatatacc
    24001 cacctatagg acctaacatc ttaatggcag catctgcaac aggagtagct gaccctgcca
    24061 agccattacc caacactcct atacttattc ccagtataag catatatatt actgatacta
    24121 caaacattac cattaccaat gctttaggta catttttctt agggttctcc atatcttttg
    24181 ctgcaacagc taaagattca aatcctgtaa aagcataaaa taaagttata gatactgcta
    24241 caaaagctga tgacgcactt tgaccttttt gtattattac aaatggggta aaattagaac
    24301 cctttataaa gaataatcct acagctataa ataaaactat tggtaataat tttcctatag
    24361 tcatgatatt atttaagtgc ttagattgtt tcattcccat aaaatttata atcccaagtc
    24421 ctacaactaa tataccaatt attatatttt tagtaagtgg ttctgctgct tgtggccata
    24481 cagatgatag tgttacagca aagaaatttg ccatagtagc ccaagcaatc atactaacta
    24541 tccacttcat aaatccaact tcaaatccaa caaaatctcc aaatgcctct tttgcataaa
    24601 gatagggccc tccatcagta tcaaaccaac tacctgcttc agcaaaacat aaagcaatag
    24661 aaacagctaa aagcatacaa aaaactaata ctaatatact tgctggacct atatctgtat
    24721 aagcatcacc aggtaacccg aatatacctg accctattat tgcattgatt cctaatagta
    24781 caatactcca aaaacctaac ttaatgcctt tttccattgt tattcccccc aaagccaact
    24841 taatatattt atatttttta tttagatgct tctttaacta ttgccataaa tatttttttc
    24901 atattatcat aatctctagt catcatttct ggatgccatt gtatccctat tacaaactca
    24961 cttccttcct tttctatagc ttcaattaaa ccgtctttag atgtagctga aacaatatat
    25021 cccggtgcta ctttgttgac aactaagtga tgaaaactat taactaaaac ttctttttct
    25081 cctaatattt catataattt tgtcccctcc tttatctgta ctgtatgtgt aggtacatta
    25141 gatagatgtt gctgattgtg tttaatataa gcaccttcaa ttaaagataa atcttgatac
    25201 aaactacctc cttcagttac atttataatt tgttctcctc tacatatccc taaaactggt
    25261 tttttcatat ctaaagcatg ttttaataat ttcatatcaa aactatctct ctttggtgat
    25321 attgccccca acttattatg aggttcttct ttccatatga gtggatttac atcttgtccc
    25381 ccagatagta taagcgcatc tatattagat acctgctcct ttataatctc atcatcatat
    25441 acaatcggta caatatatgg tatagcttta cacataacta ctgattgtat ataatcatta
    25501 tttacataag ccctttcata gccaggaaac attcctcctt catcaattag taaattacct
    25561 gatattccta ttattggata tttcttttta gccaatttct tcacccctta acttatttgt
    25621 gaacaataaa aaatgctact tctcttaaag agcaagtagc ataccaagat tttatttatt
    25681 tgaacattat aatttatttt agaaatactt tttttgaata cgagtaatat aaataacttt
    25741 atttacttat attttatttt aatcctgttt agtttgactt ttttaaattg ttttaagtac
    25801 aactaatagg taaaataggc atttagattt tcctttttat caacctatta aatactgttt
    25861 aatatatttt ttccccttct atttaattca cttccctttc taccagattc acatattatc
    25921 aaatccagct cttttaacat attaagtatt ccccttacct caccttctgt tatattgtag
    25981 cctaatccat ttattgaatt aacaatacca tttctaccta tatttttatt aagattattg
    26041 gcatttttta aacactccat tactaacttt actttctcta tagatgttct atcctttaat
    26101 atgcttattt ctttactaaa atcttcagtt atatttaata agttatgagg taaatcattt
    26161 attaaaataa catcctcaga actcataatg ttaatataca tagcagtatt ttttaattct
    26221 cttatattac ctggccattc atatgcatta ataatgtctt gaacatcttg actaattgct
    26281 ctcttccttt tcataaaatg ccttaacata acttctatat cttcttttcg ctctcttaaa
    26341 ggtggtatat ttatgggcaa tacatttatc ctatagtata aatctgctct aaatctatct
    26401 ccttttatca tttctaataa atttttattt gtagccgcaa ttactctaac atctatatct
    26461 attacatttt ctcctccaac cctcattact tgattctctt gtaatactct aagtaatttg
    26521 ctttgtaagt atataggcat gtctcctatt tcatccaaaa atatagttcc attgttagcc
    26581 tgttcaaata atcctttttt acctcctttt aaagctcctg taaaagcacc ttcttcatat
    26641 ccaaaaagtt cactttcaag taaattttct ggcatagctg cacagtttat tgctataaat
    26701 ggctgactat gtcttggtga tgcattatga atagactgag ccatgagttc ttttcccgtt
    26761 ccactttctc ccgttataag aattgtataa tcagattcag ctattttcat acctaatttc
    26821 ttagttcttt ccatcttcgt tgaaactgtt tttatatcat caaaagtata tctagcaact
    26881 tgtccctttt ctctaagctt ctttgatagg ttttgctcca attttcttat gtaagttatt
    26941 tcttgaatac aaaaataata tccagtaacc cttccaaaac tctctatatt atacttattt
    27001 acgtttaagt acttattatt aaattttata acttcatcat gtgccttact caaactaaaa
    27061 atctttttaa tattctcatt ctctaattca tctatatttt tccctataat attactttga
    27121 atatctagta tatcccctaa actattattg caaataatta tgtttccctt atctgatacc
    27181 attgccatac ctaagtttga taaatttatt atagaattta gttcatctgc ttttatagta
    27241 agttctttgt atttagtatt tattcctgaa tataaactaa ttatttcatc tgtatactta
    27301 attagcctct cagtaatttc tttatcatcc agtttgagtt ttgctattat ttgaataaac
    27361 gttgaactat caatatacct atggcctaaa tcaataactt cttctatata ttttggaact
    27421 aaacttgact ctcctggcgt tatagctatc ttaatatttc tatattcttc attgggattg
    27481 tatggtataa aattcagatg atttattcct atattgtata aattagatat tgtttccaat
    27541 acagtatgct tattatcgtt tactactaat acatctattc cttctggcaa tgaaaataac
    27601 ttatatatat ccttttgctt tatgctccta tttatagtta ttatctttga gacatcttct
    27661 acatattttt ttatttttaa tactctatca tctatcatta ccaatattat gtcatctttt
    27721 attaattcct tctctgttat tttatttaaa taataatttt ttatcattac tttatcttta
    27781 aatatcattt ctagattttt atttaagaaa cattctaatt tagacaaccc atcagttact
    27841 actgctattg ttttcatttg attactcctt ttaaaatata ctctactctt ttataatata
    27901 catctgattt tcactttaat agcacaaaaa attgtacatt tagtatacta acatattcct
    27961 ttattagttt tatgtcccta aatatcatag ttgcaatatc tatcatattt cttaaacttc
    28021 tataaagcaa aaattgtgcc aaattaataa ttttcttaat tttattctaa agttcctcaa
    28081 tagtatttac agacttagca cttatagctt ataagtcttt atattattaa tctattttta
    28141 taggttaatt gtacaaatca tatatcactt ttacccatta aatgcattat aaaaaagatg
    28201 gaataacgat tcgtcactcc accttttttg taataaaaaa ttgtatatat aaacactatt
    28261 tactactctt ctaagtacaa acagtatcta catactataa cctttcacca ttagattcta
    28321 ttacttgttt ataccagtca aaactctttt tcttaatcct agacaagtct ccacttccat
    28381 catcatactt atccacgtat ataaacccat atctcttggc catctcacca gtagatgcac
    28441 ttactaaatc tatacatccc catggagtat atccaaataa atcaacacca tcttctacag
    28501 cttccttcat ttgctctata tgtgctttta aatactctat acgatagtca tcatttatca
    28561 ctccattaga ttctttttta tcataagctc ctaaaccatt ttcaactacc atcataggta
    28621 ttctatatct atcataaatt tcatttagtg catatctaag cccttcaggg tctatttgcc
    28681 atccccattc actagactta agatatggat ttttcactcc acctaatatg ttgccacttg
    28741 tattatcttc tttagaatca ctacttacac atgtagacat ataataacta aatgtataaa
    28801 aatctacaca accttctttt aaaatttcta aatctccttt ttccattttt atttcaatat
    28861 tattttctcc aaaatatcta ttcatataag ttggatattc gccacgcact tgtacatctc
    28921 cacagaaata attcattact ctactatctc tttgagtttt aactgcatca tctggattac
    28981 aagtataagg atacattgtt gcaaatattt gcatacatcc aacttgataa tttgaatcta
    29041 tttcatgagc taacttaaca gccttagcac ttgctacaaa ttgatgatgt aacccttgaa
    29101 aacgaagttg aggtatatct acttggttta taaaatctgt agtcccttca tttaatattc
    29161 cttgtgataa gtagcctccc ataggcattg tagctgaatt tatttcatta aatgtcagcc
    29221 aatattttac ttttccttta tatcttttaa atattgtttc acagtaattc atatacaagt
    29281 ctattacatc tcttgaaacc catccattgt atttctctgt tattgcaaat ggtatttcat
    29341 agtgagatat tgttattaat ggttctatat tatattttaa acattcatct atcacatttt
    29401 cataaaattt aagtcctgct tcatttggtt ctttctccat tccagttgga aatattctag
    29461 tccatgctat tgaaaatcta aataccttaa atcccatttc tgcaaataaa gctatatctt
    29521 ctttgtatct gtgataaaaa tctattgctt catgacttgg ataaaatgtt cctttttcta
    29581 taactctcgt tattctttta cttacttttt gagaacctcc agtacacata tctgaacaag
    29641 aaggaccttt cccatctaaa ttccatgctc cttcacattg atttgctgct gttgctccac
    29701 cccataaaaa atctttttta aatgacatac ctatatcctc ctattataat tcttcaccat
    29761 ttgattctat tactttttta taccaggcaa aactcttctt tttactgcgt tttaaagttc
    29821 catttccatc attatcttta tccacatata taaatccata gcgcttcttc atttctccag
    29881 tacttgcact tactaagtca atacatcccc atggtgtgta cccccataaa tcaacaccat
    29941 ctaactcaac agcatctatc atggctttta tatgagcacg taaataatca attctataat
    30001 catcatttat atttccattt tcatctggat tatctactgc tcctagtcca ttttcaacta
    30061 taaataatgg tttttgataa cggtcatata aagagttcat agttattcta agccctaatg
    30121 ggtctatttg ccatccccac tcacttgcat ctagatatgg atttttaaga cttgcaaaaa
    30181 catttccttc tgttaaaata tttacttttg ggtctgcact tgtaagtctt gatgagtaat
    30241 atgagaacga aataaaatct actgtgttct ctttaagtat ctttttatca tcttcttcca
    30301 tcactatatt tattcctttt ctctccaatt cttttaatgc atatgttgga tattctccac
    30361 gagattgtac atctataaaa aagtaatttt ccctatcttt ttctatagat ttccatacat
    30421 ctagtgggtt acaagtatta ggataagtat ttcctgctgc taacatacac cctattttaa
    30481 attcttgatt tatgttatgt gctagtttag taactaacgc actcgcaact aattgatgat
    30541 gtgcagcttg gtattttatt tgttcttcat tttctccctc ttcaatatag agacctgcac
    30601 ctataaatgg taaatgaagt atcatgttta tttcattaaa tgttagccaa tactttacct
    30661 tacctttaaa ccttgtaaaa ataacttcac agtattttac atataagtct actagcttac
    30721 gatttctcca tgctccatat ttttttacca aatacatggg aacatcaaaa tgacagattg
    30781 ttactactgg ttctattcca tactttaaac actcatcaaa taaatcttca tagaatttta
    30841 gtccctcttc gtttggaaca tcctcaactc ctgttggaaa tatcctagtc catgctattg
    30901 acatacgata gcatttaaat cccatctcag caaataattt tatatcttct ttataattgt
    30961 gatacatatc tattgcttca tgagctgggt aatagtgttc atcatcacat tcaaacattt
    31021 tcattttccc agccaaaact ggaaatctat cttttccttg tggtgtaacg tcaacattag
    31081 ctagacctct accatcttct ttatatccac cttcacattg atttgctgct gttgctccac
    31141 cccataaaaa atcttttcta agtgccataa ttctacctcc aataatttat ttatagtaac
    31201 tttattttaa aataaaaata catatatata attcttaatt aatagaactc tcttttacgc
    31261 taaaacagtt atcaattcct gactattatt tacatgcttt tgtgtatttt ctacaatatc
    31321 cacataatca gaataattag taacaactac tggtgtttct aagcaatatc cttcttttga
    31381 aatactttct atgtcaaaag ttattaatat ctgacctttt ttaatcttat ccccttgatt
    31441 aacaaaagat ttgaaatatt taccttccaa ctgaatagta tttaacccta tatgtatcaa
    31501 tagttcacaa ccattatcag atataattcc tattgcatgt tttgttggaa ataaagtcat
    31561 tacagtccca tcaaatggtg ccactacttt tccttcttta ggtgttattg ctacaccttt
    31621 tcccaatata cctgttgaaa acgctgcatc ttgtatctgc tccaaagcct ttacttcacc
    31681 ttttattggg ctatatacta cttctctttt gtttttttct ataacgcctt gcccattatc
    31741 atctttagaa atttctacta tagcatcatc tttccaaaaa aataatgtta aaacaaatcc
    31801 tactacaata gctactatta ttgctattat agttgcataa acacctttca tatctccagt
    31861 tgttggattt atataattag gtataccaaa tattccaagt ccacccatta cataactttt
    31921 tacattcatt atccccatta cagctcctgc cacaccacca cctacacagc tatatatgaa
    31981 tggtgttttt cttggtaaag ttattccata tattgcaggc tctgttacac cacatatccc
    32041 tgaaataact gcaggtatac acagctcttt tatcttctta tcttttaatt taaagtacat
    32101 tgctgctact actgctgttt gtgcaaaaga tggcacaaat gctcccacta atattgtgtc
    32161 aaaccccata gtcataaggt ttgacatagc tattggtact aatccccaat gtagaccaaa
    32221 tattacaagc acttgccaca ataaaccagt taatgcacca cataaaattg gtgaaaagtt
    32281 aaataaagct aaaaatccat ttccaactat attagaagca taggtagcta taggccctat
    32341 aaccataaat cccaatggta aagaaactaa taaaactgcc attggtacaa agaaattctt
    32401 tactacatct ggtattattt tagcaaacac tttctccaac ttagctgcta ataatactat
    32461 taaaattact ggtactactg ttgaagtgta gttcattgct attactggta atcctaagaa
    32521 agttatatat actggtgatt caaatattgt tccagtaaat agattgtata aaggttgacc
    32581 tgtagatgct agagtatcta attgaatacc tgaatagcat aaaactgcac ctataagcat
    32641 tccaataaat ggttttaatt taaatttctt agcagctgta tatccaagaa ttattggcat
    32701 atactggaac attgcatctc caattgcatt taacataata tatgcaccat cagtttctcc
    32761 atacaaaccc aatgctgtaa atactgcatt aaatcctttt atcataccag cagcacacag
    32821 aactcctaat attggttgaa aaactcctga tattatgtct ataaacttat taagtaatcc
    32881 ttttggcgca tctgagtctg aattatctat tgtatctcct cctaatccag ccaaatcgca
    32941 cacaacttca taaacatctg gaacatgatt accaataact acttggtatt gacctgcact
    33001 tttcattaca gtaactatac catccatttt ttttagcaca tcatcatttg ctattgactc
    33061 atcttttaat ttaaaacgta gcctcgttat acagtgagat aaactgctaa tattatcctt
    33121 tcctcctaca ttcgcaatga tttcttttga tagttgttca tactttctca ttttttctcc
    33181 tccattttct ttttgaaata tgaaggtttg gccaacttaa tgtcaccacc caatatatat
    33241 aaacctaatt attaggattt atatctgact taatactttt acttaccact ctttcaacgt
    33301 gaatagttaa gtataatttt tcctcatctg ttaaatcgta gttgtatttt ttatgtataa
    33361 attcatccaa cttttctatg aatttataag catttttata tttaactttt attacattta
    33421 ataaatcatc ttcattattt tcatgtaaat tattactaac aattcgttct gcaaaatact
    33481 ttaagtgagt aataaatcta tgataataaa ctgaatcttc atcaaatact attctgtaat
    33541 ggtatttaac catatttgaa atttcttgta ttatttgtgt aatctcatat atagttttta
    33601 tactttgatc catttgtgca ttaactatat gcagtgctat aaatccagct tcatcttctg
    33661 atagtctaat ttcaaatttt ttttctatat aatccaatgc ctctaaccct attttaaatt
    33721 cactcttata aaatcttttt atatcccaaa gtaatacatt ttttacactt actccttcac
    33781 gagctctttc tattgcagta tacatatggt ctgttaaaga aatataaata ctatcattta
    33841 atcttttccc tagtttttct ttagcatata aaattatttc atctgataat tccatatatc
    33901 ttataggaat atctgctatt agttctttaa atttatcaga tatatttggg tctgaaagtt
    33961 tgaatatctt atcaatttta tcctttgata tatgctctcc tgtacgtttt ttataagcta
    34021 aacctctccc cataactact atttccttat gattttcatc agttgtaata attacattat
    34081 tattgagtat tttttcaata atcattgtga tgcccccttt attagcatta agttaaaata
    34141 aatctttttt aattatttag aatatttaaa atgtaattta ttacataaaa aaaaacctat
    34201 ttattacaca ttaaatagac tcccatgagt cctgagtaat aaataggttt agcttgtatt
    34261 taaacaatca ctatccgttt gtattttatt ttaattatta tttattatat gttagcaaat
    34321 ttatttttta gtgtcaactt tttctcaata ttttaatttt aaataaatta tataaattga
    34381 atcttcaaag tactattttt atcaataaac aaaataaaaa aggtattact ttctttaagt
    34441 aatacccatt tatttttaat ttaagtggtt tttcttaatc tcatcaagta gtccatcttc
    34501 taaataaatt tctaatgcac atcctaccat tgcctttact gctttgtgaa gtttatcata
    34561 agcttcctca gaatttgcat aatttagaaa ttctttttca tgaacatata ccattggact
    34621 tatatctaag gctatttctg tatacatagt agggcaaact tgacttacat tacctatatc
    34681 tgtagaacct actggaccat ctttaccttc taatatatct gttatacctg cctcaatcaa
    34741 attattcttt gttatctttt gaagtacttt gttatttatt atgttgtcaa aagaattttc
    34801 aaagtatcta taagaaagct tagctcctgt cattaactct gcaccttttg cacagtttat
    34861 aactttttta gttacttcat ctaaataact tctttcagca gctcttacga aaaacttaca
    34921 ggctgctttt tctggaacta tatttggagc ctctcctcca tttgaaacta taccatgtat
    34981 tctaacatct gattttacat gttgtcttag tgcatttatc ccagcaaaag taagttgtac
    35041 tgcatctagt gcattgactc ccatatgagg gtgactagct gcatgtgctg ataccccttc
    35101 aaaactaaac tctaatgaat ctatcgccaa agccttacag tttatattat taaaagcttc
    35161 tatatgcatt tgataactta catcaacatc atcaaaagct cctgccttaa ctaaatcaca
    35221 ttttcctcct gttgtttctt cagctggtgt tcctattagt acaatttttc ctttaaaatt
    35281 ttcttttaac ttacttaata ctatacaagc tcctagtgta gatgctgcaa tccagttatg
    35341 tccacaagca tgagctggtt ctttattttc tccatatcca ggaagtgcat catactcagc
    35401 taaaaatgct atggtaggtc catcattatc tcctaattca gctctaaaag cagtctccat
    35461 attacaatat ggataaactg tatcaaatcc atattccttc attttttcta ctaagtattt
    35521 tgatgatatg tattcttctt cccctaattc aggattgtta aatataaaat tacaaacttc
    35581 ttcaatttct tttccaagtt cattctctat ctcataagct ctcttcttta tactctctat
    35641 cataatttac ctcctactag aatcctagat ataatcctat tgtcaaaaat gccattacaa
    35701 gaattgttaa tactaccatt aaaggaatta cgaatctaac ccactttgac caaggaatat
    35761 tggctactgc tagtgctccc attaaaacac ctgatgtagg tgttatcaag ttagtcacac
    35821 ctataccaaa ttgataagct gtaactatca tttgtcttga tacatctact aagtcgccaa
    35881 gtggtgcaag tacaggtatt gtaagtgatg caagccctga tgctgatggt attaaaaatg
    35941 ctattaatcc ttctattaag aaagttaaat ttataaatat agtttttggt aatccattta
    36001 ataaatttgc tgcagaattt aaaattgtat ctattataaa tccattttct gctattatta
    36061 cgattccacg tgcaaatgct atacacaatc ctgctgtcat taaatctcct gctccactta
    36121 taaatgaatt aactatatca tcttgtttaa gacctgatgc tatacctgat attataccta
    36181 tagctgtaaa tatcattgca atttcagtta tataccatcc caactttaaa actccatata
    36241 tcataatcgc catacctatt gtgaaaattg ctattaccat ggcttctctt tttgtaaatt
    36301 cctttatatt tgtatcacta ttaaccattg attcttgatt tagtgatata tcgtgtacta
    36361 aagatttttt agggtctttt ttaactttat tggcatacat cataacaaat cctatagata
    36421 tagccatata tattataaac ataactactc taaactctat tccagagcct ggtgctattt
    36481 gtgataatgc ttgtgctatt cctacagaga aaggattcgt agtagctgct ccaaatccag
    36541 ctgttgctcc tataaatact gtggccactg ctgtcaatga atcatatccc atactggtca
    36601 taagtggaat aaatatcata taaaaaggta gtgtctcttc actcattcca aacgtcgtcc
    36661 cacctagtcc aaatagaatc attgaaatcg gtattattaa aaattgaaac ttcttcattt
    36721 tatttacagt atgagctata acagcttcta tagctcctgt tttattaact atcccaaaag
    36781 ctcctccaac tattaaaaca aatccaacta cttcagctgc atctataaac ccgtttatag
    36841 gtgctgtaaa tacatcagta attccttgtg gatttgatgg tgctttttca taagttccag
    36901 ctacaacaac tgaccttccg tctatatcta ctctttcaaa atttccactt ggcactatcc
    36961 aagtaagaat tgccattaac actattagca aaaatattat tgtaagtgtc tgtggcatag
    37021 taaacttttt cttttttaaa ctgctcattt ttaatctccc cactttttaa attaaatatt
    37081 ttattattgc atccctttat cctattttgt atagatgtaa aaaagctcaa catggataca
    37141 tgttgagcta aatgtgctaa atacaacata cctgcatctt ccctttatct tgtttaagat
    37201 agctctccat caaaattcgg gataattttg atgacagtac tgtacctatt aaatacagtc
    37261 ccagcactag cttcggacac tagcacttcg gcacttactc cttttatatt gcctcattgg
    37321 tttccaacct cttcaatatt aatcttagta aatgcaacct ctacctcaat ctgtttgtta
    37381 gatgaggtaa ttcttattaa tttttattaa ttcaaagtaa ttgtagcata atatgtcgag
    37441 attgtaaata ttttcagtat aataattctt actttatgat ttcattccat ttttcacaaa
    37501 taattttttc tccatataat ttaaatactg tagtatattt taaatacaca caaactttcc
    37561 acaaatttta aattattata atacctttat aataaaaaat aaaagctatg taaacttatc
    37621 aatttacaca gctttatata cattaatcta ctccaagtac agctttaagc tttgatgcta
    37681 ttgtaataac tcgtggacca aatataactt gaactgagtt tttcttttta acaactccac
    37741 ttgcacccaa ttcatttacc cagaagccat cttcatagaa tccatcaatt gaatttagct
    37801 ctaccctaag tctagttgca cagtttgtaa cattttttat attattactt cctcctaaac
    37861 cttctattat agaatgtaca agctttgtat ctttatcatc tacttcctct tctactgcat
    37921 tgttgtatgc tgttttacca gcatctacag attgtctttc tctatattct ttcttactat
    37981 aaagcttaac ttcttctgaa cttcttcctg gtgtttttat atcaaacttc ttgattgccc
    38041 atgtaaatat tatataagta actataaaaa atattggtat caaaaataat aatggtaacg
    38101 catccacttt ttgtggttgt aataaattag gaaacatatt tctaaatgat tctcccatta
    38161 tagaaacatt aaacatttct gtaaatacat acgtcaatcc tgccagtggt acatatgcta
    38221 caaaatataa taatggtgca acaaatagga atgtatactc tattggttcc gttaccccta
    38281 ccaacatggc tgttactatt gctggtatca atatacttat tgcttttttc ttattagcat
    38341 tatcagccaa cttatacata gctaaagctg ctgctggtaa tattgcaaaa tgtattaata
    38401 aacgacctgt tgtgaagtta cgagttatat aagatagtgt atctgggtct cccatttgag
    38461 ctaatcttat attattaaca ccctcgtaaa ttgctccacc tacttccatt gttccaccta
    38521 ctcttgtata ttctataggg aatgcaatta agtgatgtat accaaatggt aataatgctt
    38581 tatccattgc tccaaaagta aatgttccta ataatccact agaacctatc caattaccaa
    38641 gtttagataa tgccaaccct actggtggcc atataaagta cattgctgta cccaatacta
    38701 ttgatgctat aaacatcata atcattgaaa atcttggacc tgagaaaaaa gataatgcac
    38761 taggaagttc tatgtcaaca aatttgttat gtactgcact agctacaaat ccagctatta
    38821 tagctccaaa tatactacaa tcatatgaaa agattcctaa gaaagaacca tataaagaac
    38881 ttcgactata tgcttctgcc tctcctaatc cagatgccat aaacgcttct gctgtaactg
    38941 tatctgaatt tataccattt gctgctaaca atgtacttat aactgtgtgc attcctataa
    39001 ataaaacaat tcctcctaat gcaccccatc ctttttcctt tttagataat ccaaaggcta
    39061 tacctgtaac aaagaatatt ggtaagtatc tcattaccat aaatcctaaa tcgtttatta
    39121 ctttaaagaa tacatttata aatgaccctt cttttattag tccgctaagc cctaatgcag
    39181 aaacgttttc catatttgca aaagctgacc ctaatcctat aaatactcct gctacaacaa
    39241 gtaatataac aggtatcatc attgctccag aaagtacttg taatttatcc ttcatagtat
    39301 tatttcctcc atttttattg aaatgccagg ctatataatg catatatata tacttctatt
    39361 gctttgtata atgtatctat acttatattt tcatttgcct gatgtgaatt tggattatcc
    39421 ccctctattg tgggtccaaa tgcaactcca ttttttaaca ctcttgcata agatgcagct
    39481 ccttttgtaa aaagttcggc ttcttttcca aaaccttgtt tatatgcatt tgataattta
    39541 tttattaatt cactatctgg ttttacatac agtaatttta aatccttata tatatcaact
    39601 attaaatttt ctttttttcc aatttcatta attctattag ttataaactc ccaacttata
    39661 cccttaggat atctaaagtc tattttcata ttcaattcat ttttctctaa gaagatactc
    39721 attatacata ttgtagaaac tcccatatct acatcttctt tatacatccc taattttttt
    39781 ccatgattat catctgtaaa gtatgaattt aaaatatccc ttattataac gcctttatca
    39841 tttagttttt taattttctc taaatctttt gctaaactaa atgcacagtt atatgacctg
    39901 tgtggatttc tcgataaagc cctttctcct gtatatctta ctaatacttt accttcatct
    39961 aattcttcta tctctgtata ataagctaat gattctacta aatctttctt atcattagtt
    40021 ttaaaaacag cctcaatttt gtcacataca aatccatctt ctttgtttga ctttatatca
    40081 actaaattgt gatttctaaa tttatcttta tctattttct ttcttagatt gaagtataaa
    40141 ataccttttt ctccatttac tattgggaag tttccatctg gagaaaatgc aaattttggc
    40201 tgttcattga cactaaagta atgctccata cattcccaag tcgtctcttc agctccaccc
    40261 actatcaatc taatctttct cttcggcttt tcattaagtt ctcttaagaa aagtaatgca
    40321 tataaaatcc ctatcaaagg tcctttgttg tcattaactc ctctaccata aagatagtta
    40381 tctttttgac atactttaaa aggtttactc ttccaaagtt ctttattata tattggcacc
    40441 acatctatat gattaagcac ccctactact tcttctcctt ctccatattc tatatgaatt
    40501 gcatatccat caaagtcttt tacaacaaaa tctttgtctt ttgctatttg aataagctta
    40561 tcgaaggcat ttcttatttc tatcccaaat ggttgattta tatctgttgt actttcatct
    40621 ctaattgaag gtatttcaac tagacttcta atatcattta tgatattttc ttgattggat
    40681 tttaaataat caataattag ttctttgttt actatcatct taattattta tcaccccttt
    40741 atattaattt tacaaagcat ttcagttcat atctacatta taaattttga atattatttt
    40801 ttcaatattt caaatacttc tctaagttac cataatgata cggtaaacaa tcaacagatt
    40861 atgacaaaat catactatac ctattactaa tattttaact tatctattac gtctttttta
    40921 atgtaagttt attccaaaaa atacataaaa aaagatgtag ccttagctac atcttcttat
    40981 atctattcaa cttccattat tatgctatta atatctacat ttccaggttc tatcctatct
    41041 tttactgttc tcttaacaga atcagcatca tccattataa ctacaggagt tattatattt
    41101 aaatcatttg acgttataaa atctaaatct gcttccatta ttaaatcttt agcttttacc
    41161 ttatctcctg cctttacctt aacatcaaac ggctttcctt ctagtgacat tgtctctaaa
    41221 cctacatgta ttaatatatt tgtattatca ttactcttaa tcattattgc atgctttgta
    41281 tcaaatacat tgactatttc accatcaaca ggagaatata acttaccttc acttggtatt
    41341 attgctactc catctcccat catcttttgg gcaaaaactg catctggaac atcttctaag
    41401 ttcactaaat ttcccttaaa agggctatat attttctcaa tcttattttc ttttttctta
    41461 aatatattaa acataacaca ctctccacat tataaaattt tatgataata tttttcttaa
    41521 agcttttgct acaccatcat ttacattagt atcagttaca aaatctgcca tagattttac
    41581 ttcatctgta gcatttccca tagcaatacc aagacctgca aatttaatca tggatatatc
    41641 attttcatta tcacctatac aaataacttc tttgctatct atacctaata tatcacaaaa
    41701 ttttttaact gcaatacctt ttgaagtacc tttactcata acttcaaagt ttatctttcc
    41761 agaacttact acttctaaat cttcttgttt tttaaattct tcctttgctt ctctcaaaga
    41821 atctacgttt tctgagaaaa gtatacattt taaaatttca ttatcagctt tttctaataa
    41881 agtttcacaa cttgcatttt ctattatttc aattctgtct tcttctttat ttttactatt
    41941 tattaatcta tatggatgtt cttcacctat ttcattgttg gatataacta ttttacagcc
    42001 tttaaaatgt ttgcttaaat tatacttttc acctattgta tagattttct ttagagcttc
    42061 tttgctcaaa accttcttat actcatatcc cagtaattta aaatatgtcc cattagttga
    42121 tataatatat atatcatccc ctaaaacact tgtaaaatat ttcatagcat tataaggtct
    42181 tcccgtaact aaggctacct tgataccctt ttccatggct tcttttagtg ctttaatatt
    42241 ttcttctgat acttcaaatc ctttacccat caaagtacca tccatatcag tacaaattaa
    42301 tttatatcgc atttgtatca catcctagta agatttttct aatatttcaa caaggtcttc
    42361 tttctttcca aaaggtaaaa atcctaaaac tggacagctt gttatttgtt ctaattgttc
    42421 ctttgttaca ttaaactcag acatttttgt cttaagcccc actctcttta aaaaactttc
    42481 tatttcattt cctaaagttt tagctgcttc atcatcttcc actttttcta attcttggtt
    42541 tagtattctc gctactttgg caaatttctc tatattatcc ttatagaact tttctgcaaa
    42601 ttttgggaac actatagcca aagcctcacc atgaggaata tgtgtaactc ctcctattat
    42661 ttcacttagc ggatgtggtg ctgctgctcc agcatttgcc agtgaacttc ctcctaatgt
    42721 atctgctact gctaactttg ttctgtattc tatattattt ttatcttcca ttactttagg
    42781 tagatactta atgactaatt ccatagattt ttcagacatt atctctgtaa agtcattagc
    42841 atttggattt atataacttt caaatgcatg agtaaaagca tcaaatgctg ttgatgcagt
    42901 aattctctct ggcagagtta acataagctc tgggtcaact atacattctt ttgagaaaca
    42961 attttgatga aatattgtat ttttctcgct tcctctcgat attactgctg cttgtgtaac
    43021 ttggcttcct gttccagaag tagttgatac agctattaat ggtaattctt tttcacttac
    43081 cttatcgtat attttaaatg gatttgtata tgtactaaac atataatccc aatctatctt
    43141 ttctaaacca aaagtcaatg ctaatacttt tgcagtatct atacttgaac ctcctccaac
    43201 agctaaaaca acatctacag attctttttt agctaattca aacccttcgt ttattaattc
    43261 tacagttggg tttggctcta ctctctgaac ctcccatgac taaagtcaca gggttcctgc
    43321 ttcacagaat tttgcatact aaaatatgtc ttactaattc tccacaggcc atccccgtag
    43381 ttcctacggt tcttacataa gttatttatt ttgaattgtt attagtctta atccttcttt
    43441 taatatattt atacttgcat ttatatctct atcgtgagtt tcattacaac taggacaaat
    43501 ccattctctt atatttaagt ccttcacttc ctcgttttta tacccacatt tattgcatat
    43561 ttgtgaactt gcaaagaatt tacctacctt tacaatttgt ctaccatgcc aattagcctt
    43621 gtattctaat tgacgaataa attctgacca tgatacatct gaaattgagc gagatagttt
    43681 acgattttta atcatattct ttacttgtaa atcttctatg caaataatat cattctcttt
    43741 tattaattta gtagataatt tttgcagaaa atcatttctt tgattagcta tatgttcttg
    43801 aagtcttgca acttttaatc ttgctttatt tctatttaaa ctaccgattg tttttctcga
    43861 taattctctt tgtaatttag caagtttgtt taatgacttc ttaagatact taggattttc
    43921 tataaattct ccacaacttg agatacaaaa ttctttaatt cccaaatcta aacctatatg
    43981 attattagta ttttcaaatg cttcaatatc tacatcagta cagcataatg atacataata
    44041 tctaccactt ggttctttag atatagtagc attaagtatt ctaccttgag gtacttgttt
    44101 atcccttatc ttaaccatac caagtttagg tagttttata tattgaccac aatacataat
    44161 gtttccattt gtaaagttag ttttatatga aaatctatta attttctttg atttaaattt
    44221 aggaaatcct gctttttctt taaagaattt tttatatgca ttatctaaat cttttaaggc
    44281 gttttgtagt gaaaatttgt caggttcttt aagccaattt aattcttttt ttaaattggt
    44341 taaatcagaa ctacact
//
LOCUS       DAEMIK010000011        43387 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEMIK010000011 DAEMIK010000000
VERSION     DAEMIK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.011"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..439
                     /locus_tag="KPK87_000521"
     CDS             170..439
                     /locus_tag="KPK87_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBG2536714.1"
                     /translation="MANNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAHATEAADYEEQEENADEE"
     gene            429..1157
                     /locus_tag="KPK87_000522"
     CDS             429..1157
                     /locus_tag="KPK87_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG2536715.1"
                     /translation="MKNKLKKTMTALCAALILMGGFSVPAYAQGAATEPPAGDATNDS
                     NVVVEEKEETPPLTPKGNATLVDDFGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEAAKKPEICNCTEKCEAGKVNTSCPVCVTSMTSCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFMKPKQNVKGDTDLEDFEFED
                     YDEDEPEADTGETSEDEETEEETL"
     gene            1434..1763
                     /locus_tag="KPK87_000523"
     CDS             1434..1763
                     /locus_tag="KPK87_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG2536716.1"
                     /translation="MNFVSSRKLTWIICSIGILLAVVGVFFLPEIIPVHFANGIADDF
                     GNKVEIFLFPILLLIITLLTGKENIKYFLTHSKTFLTDMQYNLMIDGVLGIILIVEVY
                     VIYASFV"
     gene            1896..3986
                     /locus_tag="KPK87_000524"
     CDS             1896..3986
                     /locus_tag="KPK87_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG2536717.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWNYDSLPILPQEWKYTVASDKEKQFKTLKELMHRAEVSEVVNAC
                     DAGREGELIFRFVYEMADCKKPMRRLWISSMEDSAIKEGFSRLKNGEEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASERISDKAEADALKTTCEASKAVCTSLVKEKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSAFLTDDMSDTAAGIIKLLCGKFSFIAGKD
                     FTPELGKVLNSKKVSDHHAIIPTMELAKTDLAALPESERNILTLAGARLLMATAAPHT
                     FEVVTAVFECDGQSFTARGKTILCEGWKEIDRKYRAALKNKPETDDADSDTENTLPQF
                     TEGQTFENPAATVTEHDTTPPKPHNEASLLSSMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADP
                     TAFMEGIEDMARELVKTYPFLSGDKAQMFKPEREALGSCPRCGSPVYEGKKNYYCSNK
                     ECIFTMWKNDRFFEERKVAFTPKIAAALLQSGKVNVKKLYSPKTGKTYDGTILLADTG
                     GKYVNYRIELPKKK"
     gene            4117..8145
                     /locus_tag="KPK87_000525"
     CDS             4117..8145
                     /locus_tag="KPK87_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2536718.1"
                     /translation="MASLRDTVKNYQEELRDGIAWVAFWKTGRSWNAEYFHLEMSDYL
                     YPEDRSRMEEIKQADPAAVVVNGYYSGYLGEDMNLDELTAGVRRHYENGYSNIGEFIE
                     AHDDRLPPELIEEARAAAHAAGLPFSEKAYRDGEEPDPYLFDGSMSMEDYELMHRMIE
                     KERSEHMEQPILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEVFDRIGIDGKNYGEL
                     HITEYQSSIAGLAEKLTELENLDELNYLSELLKMQFDDDREKFVAAMEYGDHTRDLQD
                     IINLAQNLDCYWLYPSVQSAEDYGHYLIEELDELELPEEAKKYFMYEEYGRDAAINDG
                     GSFTEQGYIYNNRNTFTEWYDGRDVPEEYRVTPQPPVQEKEQADLDASAAIPTTATEQ
                     PPVLPIILSSEKPADKMKEITDRLEQGILGLYESDRYAYYLRTMSKFHDYSLNNTILI
                     AMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFTVKKQVEKIDPDTQKPVFDKD
                     GKPVTEEKEIKIPAFRVVSVFDISQTEGKELPALTYELTGNVEQYKDFFAALEKTSPF
                     AMGFEALSGGVKGRCNYEEKRIIINEGMDELQNIKTAIHEIAHATLHDTALAMPERPD
                     RRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRSTAASLI
                     DTIDGHFAEIQKAQDKEQTTEQTQPTQETAKQPEAEVAAPELPEETAPVQEKEAQPEQ
                     AAPAAPYYTINEAAAKRAKDMNSFSDYKQGSATAEYRHYVDEAVQLAEKQKQRVDPMY
                     HEKIDSLLDTYARKLAANMNKGYEIDARVPSILIAGGSNFPTRKKEKQNAARDSNYRE
                     WQEIQGLLDKIRSTGMGGISADDPQVVQKLERKLESLEKSQETMKAVNAYYRKHKTLD
                     GCPHLPPEELEKLKADMASSWHLEDKPFATWALSNNSAEIRRVKDRIKSLSQQKEIGF
                     VGWEFDGGKVEANTEANRLQIFFEDKPDEATREALKSNGFRWSPKAGAWQRQLTSNAY
                     YAADYVKAIAPLTGEKPTELQRAHIRQQKVAAQKQPVQEQPEQKAPQSKDTFSIYQIK
                     GGDETRDLRFEPYDRLTATGHRVDPKNYALVYSAELTPGTSLEDIYTRFNIDHPKDFK
                     GHSLSVSDVVVLHQNGQDTAHYVDSFGYKDVPEFFKEQEKALIPDDLETGETIKTPRG
                     TFYVTAMSREQMEAAGYGLHHQSDDGKYLIMGNGTRAFAVPVQPENYLKAAEQTTEQN
                     YNMIDGQINNTPTTAELEAKVKAGETISLVDLAEAVKADKERGKAVKTEKKPSIRAQL
                     RADKEKTAQKKAAKSKNHELEV"
     gene            8148..8354
                     /locus_tag="KPK87_000526"
     CDS             8148..8354
                     /locus_tag="KPK87_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBG2536719.1"
                     /translation="MIRLTVEETNLLSIYNEGGKRGLMENINAALPFMDEDMRELAKR
                     TLAKIAPLTENEYVELAIFAADEV"
     gene            8351..8716
                     /locus_tag="KPK87_000527"
     CDS             8351..8716
                     /locus_tag="KPK87_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBG2536720.1"
                     /translation="MTGVKRLTPPQSRKVNSLVKKECCNCDNGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECGTSFASTSNSVKYCPDCRKR
                     ITRRQAAERMRKRRALVTQ"
     gene            complement(8895..9146)
                     /locus_tag="KPK87_000528"
     CDS             complement(8895..9146)
                     /locus_tag="KPK87_000528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536721.1"
                     /translation="MKIVDITTCIKITEENDMYGIERYLDIGSKIKITFNSGNTLTGY
                     VQYLDYGHYPGENVLLVLKTDEDTLLGAMVCFISNIEVL"
     gene            complement(9149..10495)
                     /locus_tag="KPK87_000529"
     CDS             complement(9149..10495)
                     /locus_tag="KPK87_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG2536722.1"
                     /translation="MAVTKIKPIKSTLSKALDYIQNPDKTDGKMLVSSFGCSYETADI
                     EFGFTLAQAIEKGNNLAHHLIQSFEPGEVDYEKAHEIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGTSWKGKLKIAVDTLIPQVASFEELLQRLKAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIAGKRTKAAKAPKEQRGVSLLIDIENSIKAAQSRGYE
                     QWAKIHNLKLAAKTMNFLTEHKIEQYADLVSRIEEINAENEKTADALKSVEKRLADMA
                     VLMKHVTTYQKTKPIYEAYRRATDKDAYRAKQESSLILHEAAVKALKAAGVTKLPNLA
                     AMQEEYGKLQAQKEALYADYGKLKKQVKEYDVIKQNIDSILRQGKEPEREKGKERE"
     gene            complement(10456..10785)
                     /gene="mobC"
                     /locus_tag="KPK87_000530"
     CDS             complement(10456..10785)
                     /gene="mobC"
                     /locus_tag="KPK87_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG2536723.1"
                     /translation="MNGRKRKIQIKFYVTEEERALIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIARRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            complement(11029..11388)
                     /locus_tag="KPK87_000531"
     CDS             complement(11029..11388)
                     /locus_tag="KPK87_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2536724.1"
                     /translation="MKQSKEKFDFKAFGQAIKAARKAKGISRNQLADTLNIAPRYIAS
                     IENSGQHPSLQILYELVTLLDVSVDQFFFPEREQEKSTRRRQLDTMLDTMSETDLKIM
                     SATAKGIEEANNDRTGE"
     gene            11564..11758
                     /locus_tag="KPK87_000532"
     CDS             11564..11758
                     /locus_tag="KPK87_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2536725.1"
                     /translation="MEYISCMEAAENWGISERRVQKLCEENRIPGATKFSRIWLIPKK
                     AEKPIDRRKQHAADTTNSNR"
     gene            11727..12416
                     /locus_tag="KPK87_000533"
     CDS             11727..12416
                     /locus_tag="KPK87_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536726.1"
                     /translation="MQQILQILIVEDDILLSDMLKSLLSQNGYRVNTAYSGSEALLIL
                     EKETFDLILLDLMLPGISGEEVLTTIRKKQTTPIIGVSAKTDIDSRIDLIKNGADDYI
                     TKPFDNKELLVRMEAILRRCHRSSNNSLLKFKDLMLNPATMEVTIKDIPLVLTRYEYL
                     ILQLLMTAPNKVFTKNNIFESVWNETFCGDDNAINVHISNLRKKFAKINPNEQYIQTV
                     WGLGFKMQSNP"
     gene            12448..13374
                     /locus_tag="KPK87_000534"
     CDS             12448..13374
                     /locus_tag="KPK87_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536727.1"
                     /translation="MHNTYTIIERGGSVGLVLEVKNLSYVFPNGVGIIGIDFQIDYND
                     IYYLYGSHNSGKSLILQSILGIISPQKGSIKIFENPDFLQEKRRIGYVPQSPYYIDRM
                     TITDMMNYFSLSFGVLEKNFADILNLNLKEKKPVRHLSSFIQRKLNLWIALLGNPDLV
                     FIDDPFGGLDTQESEQLISIISYLNKNNNITFLLTGHNYELASRIATKYGVLKNGRFL
                     IELTPEKLDSECKRCIKIRTPQLLSAIPLLEKEFPQYEVLEDDLIRVFCPLSHSAKIN
                     TLLVSAGIEVSEIWIAGTMPQEYLLKLTEGGI"
     gene            13378..14154
                     /locus_tag="KPK87_000535"
     CDS             13378..14154
                     /locus_tag="KPK87_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2536728.1"
                     /translation="MIKMIQGELFRIRKNRLFKICLIVSTFFVFALALTFHYNTIKHS
                     VAAVPKASLNDYTQFLFSDYSLILPITVFLGCYFTENSRTGTCSIFLSKGITRTSIFF
                     GKLISSWIATVLYMLFNFLMAYIFILVMWVKQSYIDCSFGLIGIYLFLQILCFIGYTS
                     FIWLLSILFRYRSLVITISIFLLGTLYFYLTKISSALDLSYSLYRYWIAGLSHAMEIR
                     LVLPQLPLILLTITGYVFFPTLCSFVIYKKIDLRKHERDK"
     gene            14156..15052
                     /locus_tag="KPK87_000536"
     CDS             14156..15052
                     /locus_tag="KPK87_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2536729.1"
                     /translation="MSIFCIFLVVTSLILLLKVIIQKQQITKIKNQIDFLCSRDTQAE
                     ITLEKLDKQTEELAISINHLLKKYRLTGIKIERNDSLFRDTITSLSHDLRTPLATANG
                     YIQLLLEQPLSAEQKEYVKIANERVSAVKILLDQLFEFARIEANELKLNKQLVDANSV
                     LRDALATYYNDFEIKAITPTIDIPDSPAMIWADKDALFRVFSNIIYNALIHGDSVYTI
                     HATVERKCYYISISNHSNTICKEDIPHLFDRFYTTDSSRTKKTTGLGLAIARKLITQM
                     GGEISAHLHNEIFEICISFTIL"
     gene            15162..15338
                     /locus_tag="KPK87_000537"
     CDS             15162..15338
                     /locus_tag="KPK87_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="receptor"
                     /protein_id="HBG2536730.1"
                     /translation="MLSKISLIISIVALAVCFIAAISGILSDGMKGLQKKGKLLGVTF
                     CIYAITFLTFIISQ"
     gene            15351..16043
                     /locus_tag="KPK87_000538"
     CDS             15351..16043
                     /locus_tag="KPK87_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2536731.1"
                     /translation="MLQIKNVNLCVGSNKLLHNINLSLEQGKIYGLLGANGAGKTTLF
                     KSMLGLTAYTGEILSDKNPAKSSEFGSLIEYPAFYPKLSVMENLKLHASYIGLTNPDI
                     ENSLKQVNLWEARNKLFSQLSLGMRQRLGIARTFLGNAKYLLLDEPTNGLDPMGIKDI
                     RILLRDKLKTPKHCIIVSSHNLTEIAAIADILIFIRNGQIIEMIENKYDEKELEVLYE
                     NLMTSITTEEVQ"
     gene            16040..16795
                     /locus_tag="KPK87_000539"
     CDS             16040..16795
                     /locus_tag="KPK87_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2536732.1"
                     /translation="MKVLIKNEFTKLKREWFVLFLLLLSWLPVLTGGVGAVFNNSTKT
                     VSDLFFFMNNQFSMFFPMVIVILTGSLFYQEYKNKTYINWITYGYSKQQLFSAKIIVG
                     FVIEIVFAASLICLFHILILMLKIAGKVSVDIQEILSLTVGFALEAIIVILITTCIAA
                     IIINISRNIIVTSVIGVIYGFVSCFFIGSEFGYVIPGSFAYRLSMYFSDSSTYYELAK
                     QATIGGCISSIISFGLMLFMGIFIFTRKKKIES"
     gene            17259..17699
                     /locus_tag="KPK87_000540"
     CDS             17259..17699
                     /locus_tag="KPK87_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2536733.1"
                     /translation="MTPNRREFEKQCAFQKFCKSVLHNEACNAHEEIRRRRVREVAFS
                     DLALHEERQLYTVDKYFQDEEVEPSYQMAGKEITPKLLLEAIRALPEERRKIVLLYYF
                     EGLTDIEIGKQLNLSRSTVQYRRTSSFEQLKKYLEENADEWIEW"
     gene            17680..17937
                     /locus_tag="KPK87_000541"
     CDS             17680..17937
                     /locus_tag="KPK87_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2536734.1"
                     /translation="MNGLNGNQNDYPENALVPYPVILAASKGDPDAMKIVLQHFSGYI
                     ASLSMRKLYDERGNVYFGVDEEIRERLQAKLMRAILTFRAE"
     gene            18356..18559
                     /locus_tag="KPK87_000542"
     CDS             18356..18559
                     /locus_tag="KPK87_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HBG2536735.1"
                     /translation="MNNNDVPIWEKYTLTIEEAAKYFRIGESKLRKLAEENPVPDWVM
                     MNGNRIQIKRKQFEKMIDTVDAI"
     gene            18639..19832
                     /locus_tag="KPK87_000543"
     CDS             18639..19832
                     /locus_tag="KPK87_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2536736.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLDKQLINVDHQLLKIADVGYYVETPKTKSGNRVIPMSE
                     KVLEAFQRVLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
     gene            complement(20362..21201)
                     /locus_tag="KPK87_000544"
     CDS             complement(20362..21201)
                     /locus_tag="KPK87_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2536737.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGIL
                     GLF"
     gene            complement(21225..21983)
                     /locus_tag="KPK87_000545"
     CDS             complement(21225..21983)
                     /locus_tag="KPK87_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2536738.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(22030..22503)
                     /locus_tag="KPK87_000546"
     CDS             complement(22030..22503)
                     /locus_tag="KPK87_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2536739.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(22532..22948)
                     /locus_tag="KPK87_000547"
     CDS             complement(22532..22948)
                     /locus_tag="KPK87_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2536740.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(22995..24047)
                     /locus_tag="KPK87_000548"
     CDS             complement(22995..24047)
                     /locus_tag="KPK87_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2536741.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            24443..25123
                     /locus_tag="KPK87_000549"
     CDS             24443..25123
                     /locus_tag="KPK87_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG2536742.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(25256..25576)
                     /gene="sugE"
                     /locus_tag="KPK87_000550"
     CDS             complement(25256..25576)
                     /gene="sugE"
                     /locus_tag="KPK87_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG2536743.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            25958..26785
                     /locus_tag="KPK87_000551"
     CDS             25958..26785
                     /locus_tag="KPK87_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG2536744.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            27607..28281
                     /locus_tag="KPK87_000552"
     CDS             27607..28281
                     /locus_tag="KPK87_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536745.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            28269..29429
                     /locus_tag="KPK87_000553"
     CDS             28269..29429
                     /locus_tag="KPK87_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2536746.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKNQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            29700..30173
                     /locus_tag="KPK87_000554"
     CDS             29700..30173
                     /locus_tag="KPK87_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG2536747.1"
                     /translation="MLSFTFCSLPFWICYFWCGISDILDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIIAIALLWLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            30459..31127
                     /locus_tag="KPK87_000555"
     CDS             30459..31127
                     /locus_tag="KPK87_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536748.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            31130..33598
                     /locus_tag="KPK87_000556"
     CDS             31130..33598
                     /locus_tag="KPK87_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2536749.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYTLSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITNSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLTC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(33838..35262)
                     /locus_tag="KPK87_000557"
     CDS             complement(33838..35262)
                     /locus_tag="KPK87_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2536750.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(35281..35973)
                     /locus_tag="KPK87_000558"
     CDS             complement(35281..35973)
                     /locus_tag="KPK87_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536751.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(36028..36804)
                     /locus_tag="KPK87_000559"
     CDS             complement(36028..36804)
                     /locus_tag="KPK87_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2536752.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(36801..37571)
                     /locus_tag="KPK87_000560"
     CDS             complement(36801..37571)
                     /locus_tag="KPK87_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2536753.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(37568..38272)
                     /locus_tag="KPK87_000561"
     CDS             complement(37568..38272)
                     /locus_tag="KPK87_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2536754.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(38527..39039)
                     /locus_tag="KPK87_000562"
     CDS             complement(38527..39039)
                     /locus_tag="KPK87_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536755.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            39365..39622
                     /locus_tag="KPK87_000563"
     CDS             39365..39622
                     /locus_tag="KPK87_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536756.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            39765..40391
                     /locus_tag="KPK87_000564"
     CDS             39765..40391
                     /locus_tag="KPK87_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG2536757.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(40638..41417)
                     /locus_tag="KPK87_000565"
     CDS             complement(40638..41417)
                     /locus_tag="KPK87_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2536758.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSFVLRLVGKNMQIATTKFFVENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(41485..41946)
                     /locus_tag="KPK87_000566"
     CDS             complement(41485..41946)
                     /locus_tag="KPK87_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2536759.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(41922..42599)
                     /locus_tag="KPK87_000567"
     CDS             complement(41922..42599)
                     /locus_tag="KPK87_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2536760.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            42832..43218
                     /locus_tag="KPK87_000568"
     CDS             42832..43218
                     /locus_tag="KPK87_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2536761.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVARESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
BASE COUNT        14366 a         8121 c         7352 g        13548 t
ORIGIN      
        1 gaccatgtgg ggcttgtgct tggcagggac ggcagccgcg tctataccgt tgagggcaat
       61 tccggcgacg cctgcaagat aaagagctat gaccttaact atcaatgtat caaaggctac
      121 gggctgatga actggtaaag cagccccaaa aagaaaggag aaatgaccta tggcaaacaa
      181 taaaatcgac cgtatcaata aggaaatcgc aaagacccgc gagaaaatca cagagtatca
      241 gaacaagtta aaaggactgg aagcgcagaa aaccgaagcg gaaaaccttg aaatcgtgca
      301 gcttgtacga gctatgcgtc tgaccccgca ggaacttaac gctatgctgt ccggcggcgg
      361 tattcccggc atgaacgccg cacacgctac cgaagcggca gactacgaag aacaggagga
      421 aaatgcagat gaagaataaa ctgaaaaaga ccatgactgc cctttgtgcc gcccttatcc
      481 ttatgggcgg tttttctgtc cctgcctatg cacagggcgc agccacagag ccgcccgcag
      541 gggacgcgac caacgacagc aacgtagttg tggaggaaaa agaggaaacg ccgcccctta
      601 ccccaaaggg aaacgctaca ctggttgacg atttcggcgg caataagcag cttatcactg
      661 ttaccaccaa aagcggcaac tatttctata tcctcattga ccgcgccgcc gagggcgaac
      721 agaccgtaca tttcctaaat caagtggacg aagccgacct tatggcactg accgaaaacg
      781 gcgaagctgc caaaaagccg gaaatctgta actgtacgga aaaatgcgaa gccggaaagg
      841 taaacacgtc ctgcccggta tgtgttacca gtatgacaag ctgcacaggc aaagaaccca
      901 ccccaacccc aaccgagcag ccggaagaac cgaaagaaaa aggcggcaat cccggtgtgg
      961 tgcttgcctt agtcctcttt gccctgcttg gtggcggcgc agcgttctac tactttaagt
     1021 ttatgaagcc gaagcagaat gtaaagggcg ataccgacct tgaagatttt gaatttgagg
     1081 actacgacga ggacgagccg gaagcagata ccggggaaac ttccgaagat gaagaaacgg
     1141 aggaagaaac cctatgaccc tgtttaccga caatcccttt gaaaagatga tgatacagaa
     1201 accgaacggg cggcgtgata atgcgccgcc tgttccttat cccccggctt gcgcttcctg
     1261 cccttataag ggacagtccc cttgtgtggg gtactgtatc agaaagattg aaaccaaaca
     1321 accgacagaa cgataaaaag atatacttac gatatgaaat gtgatataat tttccgcaaa
     1381 tagaaaatag caatgctaaa caaatctaaa tttgatagaa agaaggaatt tttatgaatt
     1441 ttgtaagtag tagaaaatta acatggataa tttgcagtat tggtatttta ttagcggtag
     1501 taggtgtgtt tttcttacca gagattattc ctgttcattt tgcaaatgga atagcagatg
     1561 attttggcaa taaggtagag atttttttat ttcctatttt gttattgata attacacttt
     1621 taactggaaa agaaaacatt aaatacttct tgacacattc aaaaacattc ttgacagata
     1681 tgcagtataa tttgatgatt gatggcgttc ttgggattat tttgattgta gaggtatatg
     1741 ttatctatgc ttcatttgtt taaacatatc gaagatagac tctgactttt tcatacaatt
     1801 tattattttc atatcgcaca attttataca atcacttgga ggagcaatcg aaaaatcggt
     1861 tgctcttttc ttttacccga aaggaggatt tatcattgat tttagttatc gcagaaaagc
     1921 ccagtgtggc gcagacaatc gctgccgtac ttggggcaaa ggaaaagaaa gacggatttc
     1981 tcacaggaag cggctatatc gtttcttggt gcgtgggaca tttggtaggg cttgcggaag
     2041 ctgccgccta cggggaacaa tataaaaagt ggaactatga cagcttaccg attttgccgc
     2101 aggaatggaa gtacaccgtc gcttctgaca aggaaaagca attcaaaacc ctaaaagagc
     2161 ttatgcaccg ggcagaagtt tccgaagtcg tcaatgcctg cgacgcaggg cgcgagggag
     2221 aactcatttt ccgttttgtc tatgaaatgg cggactgcaa gaaacctatg cgccgccttt
     2281 ggatttcttc tatggaggac agcgcaatca aagagggctt ttcccgtctg aagaacggcg
     2341 aggaatacga cgcgctcttt gcttccgcat tatgcagggc aaaggcagac tggctaatcg
     2401 gtatcaatgc cacccgtctt ttctctgtcc tttacaatca taccttgaac gtgggacgcg
     2461 tacagacccc gaccttaaaa atgcttgttg accgggacgc agcgattacc acattcaaga
     2521 aagaaaaata ctaccatgtg cgccttgcct tatccggcgc ggaagctgca agtgaacgaa
     2581 tttcagataa ggcagaagcc gacgcattaa aaacgacttg tgaagcgtca aaagcagtct
     2641 gtacttccct tgtgaaagag aaaaagaccg cagccccgcc gaagctcttt gaccttactt
     2701 ctttacagag ggaagcaaac cgcctgttcg gatataccgc aaagcagacc cttgaccttg
     2761 cacaagccct gtatgaaaaa agacttctta cttatccgag aacagacagc gcatttctga
     2821 ccgacgacat gagcgacaca gcggcgggca ttatcaagct gctttgcgga aagttttctt
     2881 ttatagcggg aaaagacttc acgccggagc ttggaaaggt gctgaacagt aaaaaggtat
     2941 cagaccacca tgccattatc ccgactatgg agcttgcaaa gaccgacctt gccgcgctgc
     3001 cggaaagcga aaggaatatc cttacccttg ccggggcgcg tctgctcatg gcgaccgctg
     3061 caccgcatac ctttgaagtg gttacagcag tttttgaatg tgacggacag tcctttaccg
     3121 caaggggcaa gaccattctt tgtgagggtt ggaaagagat tgaccgaaaa tacagggcag
     3181 ccttaaagaa caagcccgaa acggacgacg cagacagcga cacagaaaat accctgccac
     3241 agtttaccga gggacagacc tttgaaaatc cggcggcaac ggtaacggag catgacacaa
     3301 caccgccaaa gccccacaac gaagcgtcgc tcctttcttc tatggagcgc gccggaagcg
     3361 aggacaccga cccggacgcc gaacgcaaag ggcttggaac tcccgccacc cgcgccgccg
     3421 tcattgaaaa actggtaaag ggcggctttg tggagcgaaa aggcaagcag ctacttccca
     3481 caaaagacgg tatcaacctt gtgtgcgtcc tgccggacac cttgacaagc ccgcagctta
     3541 ccgcagaatg ggaaaacaat ctgacgcaga ttgcaaaagg caaggcagac cccaccgcat
     3601 ttatggaggg tatcgaggat atggcgcggg aactggtaaa gacctatccg tttctttctg
     3661 gtgataaagc gcagatgttc aagccggaac gggaagcatt gggaagctgc ccccgctgcg
     3721 gctctcctgt ctatgaggga aagaaaaact actattgcag caataaagaa tgtatcttta
     3781 ccatgtggaa aaatgaccgt ttttttgagg aacgcaaagt agcctttacc cccaaaattg
     3841 ccgcggcact cttacaatcc ggcaaggtaa atgtgaaaaa gctctattcc ccaaagacgg
     3901 gcaaaaccta cgacggaact atcttattgg ctgatacggg cgggaaatat gtcaactacc
     3961 gcattgaact gccgaagaaa aaataactga atagcaagca taggaaaaga gtaccctttt
     4021 ccgtaaaatc cctgctttct ctaccgagaa cggcggggat ttttgcttgt tgggaagttt
     4081 cccaaaccct ttaagagcga agaaaggagg ttacacatgg caagtttacg ggacaccgta
     4141 aagaactatc aagaagaatt aagggacggt atcgcttggg tggcgttttg gaaaacggga
     4201 cgttcttgga acgccgaata tttccacctt gaaatgagcg actaccttta cccggaggac
     4261 agaagccgca tggaagaaat caaacaggct gaccccgccg ccgttgtggt aaacggctat
     4321 tattccggct atcttggcga ggatatgaac cttgacgagc tgaccgccgg ggtgcgccgc
     4381 cactacgaaa acggatatag caatatcggg gaatttatcg aagcccacga cgacaggctg
     4441 ccgccggagc ttatcgagga agcaagagcc gccgcccacg ctgcggggct tcctttctct
     4501 gaaaaagcct accgggacgg cgaagaaccc gacccctatc tatttgacgg tagcatgagc
     4561 atggaggact acgagcttat gcaccgcatg attgagaaag aaaggagcga acacatggaa
     4621 caaccgattt taagcgggta tctttctaat cttggaaagt acaccgaggg cagacctgcg
     4681 ggcgaatggg tatcattccc cacgactgcc gagcatttga aagaagtctt tgaccgtatc
     4741 ggcatagacg gcaaaaacta cggggagctg catatcacag aataccagtc ctctattgcg
     4801 ggactggcag agaaattgac cgagcttgaa aaccttgacg agctgaacta tttgagcgaa
     4861 cttttgaaaa tgcagtttga cgacgaccgg gaaaaatttg ttgcagctat ggaatacggc
     4921 gaccatacaa gggacttgca ggacattatc aatcttgcac aaaaccttga ttgttactgg
     4981 ctttatccgt ccgtacagag tgcggaagat tacgggcatt atctgattga agaactggac
     5041 gagttggagc tgcccgaaga agcaaaaaaa tatttcatgt atgaggaata cgggcgggac
     5101 gctgctatca atgacggggg cagctttacc gagcagggct atatctataa taaccgcaac
     5161 acctttacag aatggtacga cgggcgcgac gtgccggagg aataccgggt aacgccacag
     5221 ccgccagtac aggaaaagga acaagcagac cttgacgctt ccgcagccat accgaccacc
     5281 gccacagagc agcccccggt tctcccgatt atcctatctt ccgaaaagcc cgccgataaa
     5341 atgaaagaga ttaccgaccg actggaacaa gggattttag gactttatga aagcgaccgt
     5401 tacgcctatt atctgcgtac catgtctaaa ttccatgatt acagccttaa caatacaatc
     5461 cttatcgcca tgcagggcgg caaccttgtg aaaggctata aacaatggga aaaggaattt
     5521 gaccgccatg taaagccggg agaaaaagct atcaagatac ttgcaccgtc cccgtttact
     5581 gttaagaagc aggtggaaaa aatcgacccg gacacccaaa agcctgtttt cgataaggac
     5641 ggaaagcctg ttaccgaaga aaaagaaatc aagatacccg cctttcgtgt ggtatctgtc
     5701 tttgatattt cacagaccga gggaaaagaa cttcctgccc ttacctatga gctgacgggc
     5761 aacgtggaac aatacaagga ctttttcgct gcccttgaaa agacttcccc ctttgctatg
     5821 ggatttgaag cactaagcgg cggcgtaaag ggacgctgca attatgagga aaagcgtatc
     5881 atcatcaacg agggtatgga cgaattgcag aatatcaaga ccgcaatcca tgaaatcgcc
     5941 cacgccacgc tgcatgatac cgcccttgcc atgccggaac gccccgaccg ccgcacccgc
     6001 gaggtacagg cggaaagcgt cgcctatgct gtctgccaac attacgggct tgatacgtcg
     6061 gactattctt ttgggtatat cgccgggtgg agcagcggaa aagaacttgc agaactgaaa
     6121 ggctctcttg aaacaatccg cagtaccgcc gcaagtctga ttgataccat agacggacat
     6181 tttgcagaaa tccaaaaggc acaggataag gaacagacca ccgaacagac acagcccaca
     6241 caggaaaccg ccaaacagcc cgaagcagaa gtagccgcgc cggagcttcc cgaagaaaca
     6301 gccccggtac aggaaaaaga agcacagccg gaacaggctg cacccgccgc cccttattac
     6361 actatcaatg aagctgccgc caaacgtgca aaggatatga acagcttttc cgattataag
     6421 caaggcagcg cgacggctga atacaggcat tatgttgatg aagccgtaca gcttgcagaa
     6481 aagcagaaac agcgggtaga cccgatgtac catgagaaaa tcgacagcct gcttgacacc
     6541 tacgcccgga aactggcggc gaacatgaac aaaggctatg agattgacgc gcgtgttccc
     6601 tctatcctga ttgcgggcgg ctctaacttt cccacaagga agaaagaaaa gcagaacgca
     6661 gcccgcgaca gcaattaccg ggaatggcag gaaatacagg gattgcttga taaaatccgc
     6721 agcaccggca tgggcggtat cagcgcagac gacccacagg tagtacagaa attagaaagg
     6781 aaactggaaa gccttgaaaa atcacaggaa accatgaaag ccgtaaacgc ctattaccgc
     6841 aagcataaga cccttgacgg ctgcccgcat ttaccgccgg aggaactgga aaaactgaaa
     6901 gcagacatgg cgagcagttg gcatttggag gacaagccct ttgcgacttg ggcgttatcc
     6961 aacaacagcg cagaaatccg gcgcgtgaaa gaccgtatca aatccctttc acagcaaaaa
     7021 gaaatcggtt ttgtgggttg ggaatttgac ggcggcaagg tggaagcaaa caccgaagca
     7081 aaccgcctgc aaatcttctt tgaagataag ccggacgaag ccacgcggga agccctaaaa
     7141 agtaatggct tccgttggtc gcccaaagca ggggcatggc agcggcagct taccagtaac
     7201 gcctactatg cggctgatta tgtcaaggcg attgcacccc ttaccgggga aaagcctacc
     7261 gagttacagc gggcgcatat ccggcagcaa aaggtagctg cacagaaaca acccgtacag
     7321 gagcagccgg aacagaaagc cccacagagc aaagacacct tttccattta tcagataaaa
     7381 ggcggggacg aaacaagaga tttgcgcttt gaaccttatg accgtctgac tgccacagga
     7441 caccgggtag acccaaaaaa ctatgccctt gtctattccg cagagcttac gccggggact
     7501 tcccttgaag atatttacac ccgctttaat atcgaccacc cgaaagattt taagggacac
     7561 agcctttccg tttccgacgt ggtggtactt catcagaacg ggcaggacac cgcccactat
     7621 gtagacagct ttggttataa ggacgtgccg gaatttttca aggaacagga aaaagccctc
     7681 attcccgacg acttggaaac gggcgaaaca atcaaaacac cgagagggac tttctatgta
     7741 accgccatga gccgcgagca aatggaagct gccggatatg gattacacca ccagtccgac
     7801 gacggaaaat atctgattat ggggaacggt acaagggctt ttgctgttcc cgttcagccg
     7861 gaaaactatc tgaaagctgc cgagcagacc acagagcaaa actacaatat gattgacggg
     7921 cagataaaca acactccgac caccgcagaa ctggaagcaa aggtaaaggc aggagaaacc
     7981 atttcccttg ttgaccttgc ggaagctgtt aaggctgaca aggaacgcgg caaagcagta
     8041 aaaacggaaa agaagccctc tatccgggcg cagcttagag cagacaaaga aaaaaccgca
     8101 cagaaaaaag cagcaaaatc aaaaaatcac gaattggagg tatgaacatg attagactga
     8161 ccgttgaaga aacaaacctg ttgagcattt acaatgaggg cggcaagcgc gggcttatgg
     8221 agaacatcaa cgctgcgctg ccattcatgg acgaggatat gcgggaactt gcgaaacgta
     8281 cccttgcgaa aatcgcacct ctgacagaaa atgagtatgt ggagcttgcc attttcgccg
     8341 ctgatgaagt atgaccgggg ttaagcggct tacgccgccc caaagccgga aagtcaacag
     8401 ccttgtaaaa aaggagtgct gcaactgcga taacggacac tgtattttac tggacgacgg
     8461 ggaggaatgt gtctgcccgc agcttatttc ctattcgctt ctttgcaaat ggtttcagac
     8521 cgccgtattg ccccttgata agctgctgta tgcagagctt ttcaagaccg aggataaaaa
     8581 gcgttgtacc gagtgcggaa cctcttttgc gtccacctct aacagcgtca aatactgccc
     8641 ggactgccgg aagcgtatta cccgcagaca ggcagcggag cgcatgagaa aacggcgcgc
     8701 ccttgttacg cagtaggggc aaaaaaagtc ttgatttacg gggcataaca gatacgaaac
     8761 ttgcataagc gatacgaaat accttttccc ttaaaatcag ggttctactg cgtaacatat
     8821 caaatcatct gcacccacaa aaaacgggcg cggcggctat gattacatgg ctgctgcgtc
     8881 cgttcttttt ttgtctacaa tacttctata ttcgatatga aacataccat tgcgccgagt
     8941 aatgtatctt catctgtttt taagacgagc aatacattct ctccgggata atgtccgtaa
     9001 tccaaatact gcacatatcc tgtaagtgtg tttccgctgt taaatgtgat ttttatttta
     9061 ctgcctatat caagatacct ttctatgccg tacatatcat tttcttctgt tattttgata
     9121 cacgttgtaa tgtcaacaat tttcataatt attcccgttc cttccctttc tcccgttccg
     9181 gttctttgcc ctgccgcaaa atgctgtcta tgttctgctt gataacgtcg tattccttga
     9241 cctgtttttt cagctttcca taatcagcgt aaagggcttc tttctgcgct tggagcttcc
     9301 cgtattcttc ctgcatagcg gcgagattgg ggagcttagt aacgcccgcc gctttcagtg
     9361 cctttactgc cgcttcgtgg agtatcagac tgctttcctg ttttgcccgg tatgcgtcct
     9421 tatccgttgc cctgcggtat gcttcataaa taggttttgt cttttggtag gtggtaacgt
     9481 gcttcatcag tactgccatg tctgcaagac gcttctccac gctctttaat gcgtctgctg
     9541 tcttttcatt ttccgcattt atttcctcaa tccggctgac taaatctgcg tactgctcaa
     9601 tcttatgttc tgtcaagaaa ttcatggtct ttgctgccag tttcagatta tggattttcg
     9661 cccactgttc atagccccgg ctttgcgctg ccttgatact gttttcaatg tcgataagca
     9721 gcgatacgcc gcgttgctct ttgggagctt tcgccgcctt tgtgcgcttg cctgcaatcc
     9781 gttctttgat tgcttcctct gtataatctg caccgagagt ttttaagcgg gtaaatcgtt
     9841 cctgtccggg agcgcggcag gagatatatt ttcccggttt tatctcatag cccgctgcct
     9901 ttaaccgctg caataattct tcaaaacttg ccacttgggg gataagtgta tcaacggcaa
     9961 ttttcagctt gcctttccaa cttgtaccga ccttttccgc ttgatattct gcatagcttt
    10021 ttcccttgct gcctttgccc ggaacgacaa cagacagccc gttttcccgg cacagcttat
    10081 cactcatgtt ccgtatgcca tagtagctgc gtttattgga attgtattta tggtagtcaa
    10141 cgaaattgac cgcacaaaaa atgatatggt tatggatatg ccccttgtct atgtgggtgg
    10201 ttaggacata ttcatgctgt ccctttgtta ccgcgtcggc aagctgtttt ccgatttcgt
    10261 gggctttctc atagtctact tctcccggct caaaggactg tatcaaatgg tgggcgagat
    10321 tgttcccttt ctctatggct tgtgcaagcg taaatccgaa ctcaatatct gccgtttcat
    10381 agctgcaccc aaaggaggac acaagcattt tcccgtctgt tttatccggg ttttggatat
    10441 agtcaagggc tttgctcaac gtgcttttaa taggcttaat ctttgtaacc gccatatctc
    10501 tgcaagcacc ccctttattt cctctatgtc ctcttggtag acgctgcctg tcgcattgac
    10561 acgccttgca atctgattga tgttgacccc gattttctgt atctccgctg tcatggcttt
    10621 tatgtcgcta tggtctgtct gaatgatata cccgtcaatg gcaatcttgc gaagatacgc
    10681 cgccatgttc cgggtgggaa cgagcttcat tttctgctca atcaatgccc gttcttcctc
    10741 tgttacatag aatttgattt gtatttttct tttgcgtccg ttcatgcaat cgctcctttc
    10801 cattccgagg gtttgggaca cttcccaaca agcaattttc aaccgacgca ccgcaggacg
    10861 ggagggcgaa aatacggaaa agggtactct tttcctatgc ttgctaagaa agttttttcc
    10921 tcacttatca ctacaacgaa actcccagta tgccaaaaaa gagcaaaaga aaaacgccgc
    10981 aatcctgcga cgtttcaatc actttatgca gacaaaaacg agggaacttt attcccccgt
    11041 cctgtcgttg ttggcttcct ctatgccttt tgcagtagca gacatgattt ttaagtctgt
    11101 ttcgctcatt gtgtcaagca tggtatcgag ctgacggcgg cgtgtggact tctcctgttc
    11161 tcgttccggg aaaaagaact ggtctactga tacgtcaagc aaagtcacaa gctcatacaa
    11221 aatctgtaaa cttgggtgct gcccgctgtt ctcaatggac gcgatatatc ggggcgcaat
    11281 attcagcgtg tcggctaact gattgcgtga tattcccttt gcttttcttg ctgctttaat
    11341 ggcttgtccg aaagccttaa aatcaaattt ctctttactc tgcttcataa ttatttcacc
    11401 cagttacatt ttactgttcc tcttttcttt gagatatgat tacacatatc gtagtattga
    11461 ctaaaataga gcatattatt tatgcgccac ttcttgtatt tgttcggttg aacgtatata
    11521 ataatattgg cgaagttaat ttctgatgaa agggtgtcaa aatatggagt atatatcttg
    11581 catggaagcc gcagaaaatt ggggaatttc tgaaagacga gtacaaaagt tgtgtgaaga
    11641 aaacagaata cccggagcga ctaaattcag ccgtatatgg ctgataccaa aaaaagcaga
    11701 aaaaccgatt gacaggagga aacagcatgc agcagatact acaaattcta atcgttgaag
    11761 atgatatttt actaagtgat atgctaaaaa gtctattatc tcaaaacgga tatagagtta
    11821 atacagctta ttcggggagt gaagcacttt taatccttga aaaagaaact tttgatttaa
    11881 ttttacttga tttaatgctg ccgggaatat ctggcgaaga agtattgaca acaataagaa
    11941 aaaaacaaac tacgccgatt attggtgtgt ctgcaaaaac ggatattgac agtagaattg
    12001 atttgataaa aaacggtgct gatgattata ttacgaagcc ttttgataat aaagaactgt
    12061 tagtgcgaat ggaagctatt ttacgtcgct gtcatagaag cagcaataac tccctattaa
    12121 aatttaagga cttaatgctt aatccggcaa caatggaagt gacaataaaa gatattcccc
    12181 ttgttctcac aagatatgaa tatttaattt tacagttatt gatgactgcc cctaataagg
    12241 tctttaccaa aaataatatc tttgaaagtg tatggaatga aactttttgc ggagatgata
    12301 atgcaatcaa tgtccacata agcaatcttc ggaaaaaatt tgctaagata aatccgaatg
    12361 agcagtatat acagacagta tggggattgg gctttaaaat gcagagtaat ccttagaaaa
    12421 tgcttataaa taaagctata atctttaatt cacaatacat atacgataat agaaagaggt
    12481 ggaagtgtgg ggcttgtttt agaagtgaaa aatttatcct atgttttccc aaatggcgtt
    12541 ggtattattg gcatagactt tcaaatagat tataacgata tttattattt atatggaagt
    12601 cataactccg gcaaaagttt aatattacaa tcaatacttg gtataatttc tccacaaaag
    12661 ggaagcatta aaatttttga aaatcccgat tttttacaag agaaaaggcg tattggatat
    12721 gtaccacaaa gtccatatta cattgacaga atgactataa cagatatgat gaattatttt
    12781 tctttatcat ttggggtatt agaaaaaaac tttgcagata tattaaacct taatctgaaa
    12841 gaaaagaaac ctgttcgtca tttatcttcc tttatccaac gaaaactaaa tttgtggatt
    12901 gctcttttgg gaaatcctga tttggttttt atagacgacc catttggagg actcgatact
    12961 caagaaagtg aacaacttat ttcaatcata tcttatctga ataagaataa taatatcact
    13021 tttttgttga caggacataa ttatgagctt gcttcccgaa ttgcaacaaa atatggagtt
    13081 cttaaaaatg ggcgtttctt aatagaactc actccagaaa aattagacag tgagtgcaaa
    13141 cgctgtatca aaataagaac gccacaatta ctttctgcaa ttcctttatt agaaaaagag
    13201 ttcccgcaat atgaggtttt agaagatgat ttaatcagag tattttgccc cttatctcat
    13261 tctgctaaga taaatacatt attagtatcc gccggaatag aggtatcaga aatttggatt
    13321 gctggaacta tgccacaaga atatcttcta aaattaacgg aaggaggaat atagcctatg
    13381 attaaaatga tacaaggaga gctttttcgt attcgcaaaa atcgtttatt taagatatgt
    13441 ttgatagtat ccacattttt tgtttttgca cttgcattga cttttcatta caatacaata
    13501 aagcactccg ttgcggctgt tccgaaagcg tccttaaacg attatacgca atttttgttt
    13561 tcagattata gtttaatact tcctattacg gtattcttgg gctgctactt cacagaaaac
    13621 agccggactg gtacttgttc gattttcttg tcaaaaggaa ttacaagaac atcaattttt
    13681 tttggtaaac taatttcttc atggattgcc actgtcctgt atatgctgtt caattttctt
    13741 atggcatata tttttatctt agttatgtgg gtaaaacaat cctacattga ttgctcgttt
    13801 gggctaatcg gtatctacct gtttttacag attttatgct ttataggcta tacttctttt
    13861 atttggctac taagtatctt atttcgttat cgtagccttg ttatcacaat ttctattttt
    13921 ttattgggga cattatactt ctatcttaca aaaataagtt cagccttaga cttatcctat
    13981 tcactatatc gttattggat agcagggtta tctcatgcaa tggaaatcag attagtactg
    14041 ccacaattac cgctcatatt gcttaccatt accggatatg tgtttttccc tacattatgt
    14101 tcattcgtta tttacaaaaa aatagatttg cgcaagcacg aaagggataa ataatatgag
    14161 tatattttgt atttttcttg tagttacatc tttgatatta ctattaaaag taattattca
    14221 aaagcaacaa ataactaaaa tcaaaaatca aatagacttt ttatgttcca gagatacaca
    14281 agcagaaata accttagaaa aactggataa acagacagaa gaacttgcta ttagtatcaa
    14341 tcatcttttg aaaaaatata gattgaccgg gataaaaatt gaacgaaatg acagtttatt
    14401 tcgtgataca atcacaagcc tttcccacga tttgcgtacc ccgctcgcaa ctgcgaatgg
    14461 ctatatacaa ctacttttag aacaacctct atccgctgaa cagaaagaat atgtaaaaat
    14521 agcaaatgag cgagtttcgg cagtaaagat attgcttgac cagctttttg aatttgcgag
    14581 aattgaagcg aatgaactaa aactaaataa acagttagta gatgccaaca gtgttttaag
    14641 agatgcctta gcaacatact ataatgactt tgaaataaaa gctattaccc ctactattga
    14701 tattcccgat agtccggcta tgatttgggc agacaaagac gcattattta gggttttctc
    14761 aaatatcata tataacgctt taatacatgg cgatagtgtt tatacaatcc atgcaacagt
    14821 tgaaagaaag tgttattata tcagtatttc caaccattca aatacaatct gtaaagagga
    14881 tatacctcat ctgtttgacc gtttctatac aacagacagt tctcgtacaa aaaaaacaac
    14941 gggactgggg ttagcaatcg cgcgtaaact gattacacaa atgggtggag aaatatctgc
    15001 acatttacac aatgaaatat ttgaaatatg tatttccttt acaattttat aaaaacaggg
    15061 tggctatggc taccctgttt ttatctttag acttttctta gacttttcct aaaaaaatca
    15121 taactctctt tgttactata cattacaagg gaggtggtca aatgttatct aaaatttcct
    15181 taatcatttc gattgttgca cttgctgtat gttttattgc agcaatcagt ggtattttaa
    15241 gcgacggtat gaaaggcttg caaaaaaaag gaaaactact tggtgttaca ttttgtattt
    15301 atgcaataac ttttttgact tttataatct cacaataatg gaggtctaca atgttacaaa
    15361 ttaaaaatgt aaatctttgt gtcggaagca acaagctact tcacaatatc aatctttctt
    15421 tagaacaagg aaagatatat ggactacttg gtgcaaatgg cgcaggcaaa accacattgt
    15481 ttaagtctat gttaggactg actgcttata caggcgaaat tctttcagat aaaaatcccg
    15541 ctaaaagtag tgagtttgga agtttaattg aatatcctgc attttaccca aaactttccg
    15601 tcatggaaaa tctgaaactt catgcaagtt atattgggct tacaaatccc gacatagaaa
    15661 attctttgaa acaagtcaat ttatgggaag caagaaataa gctcttttca cagctttctc
    15721 ttggcatgag acagcgactt gggattgccc gcactttttt aggaaatgcg aaatacttac
    15781 tattagatga gccaacaaat gggcttgacc ctatggggat aaaggacatt cggatattat
    15841 tacgtgataa attaaaaacc ccaaagcact gtattattgt atcaagccat aatctcacag
    15901 aaattgcggc aatcgcagat atacttatct ttatccgtaa tgggcagatt atagaaatga
    15961 ttgaaaacaa atatgatgag aaagaattgg aagttcttta tgaaaatctt atgacttcca
    16021 taactacgga ggaagtacaa tgaaagtttt aataaagaac gaatttacta aattgaaacg
    16081 tgaatggttt gtattatttt tacttctcct ttcatggcta cctgttttaa ctggcggagt
    16141 gggggctgtc tttaacaata gtacaaaaac cgtaagtgat ttatttttct ttatgaataa
    16201 tcaattttcc atgtttttcc ctatggtaat tgttattttg acaggttcgc ttttttatca
    16261 agaatataaa aataaaacct atataaactg gattacttac ggatattcaa aacaacagtt
    16321 attcagtgca aaaattatcg tcggatttgt tattgagatt gtatttgccg cctcgctaat
    16381 atgcctgttt catattttga ttttaatgct gaaaatcgca ggaaaagttt ctgttgatat
    16441 tcaagaaatt ttatccttaa ccgtaggatt tgcattagaa gctataattg ttattctgat
    16501 tacaacttgc atagcagcaa ttatcatcaa tataagcaga aatatcattg taacgagtgt
    16561 tataggcgta atttatggat ttgtgtcctg cttctttatt ggaagtgaat ttggatatgt
    16621 tattccgggg agttttgcct atcgtttatc tatgtatttt tcggatagtt ctacatatta
    16681 tgaattggca aaacaggcaa caatcggtgg ctgcattagc agtataattt catttggact
    16741 aatgcttttt atgggaatat ttatttttac ccgaaagaaa aaaatcgaaa gctaatgatt
    16801 gccggacgac ggcaaaagaa aaaaaagccg tcgtaatgca gatatatttt cacgcgcctt
    16861 tatagcggtg gctttgattt tagagccgcc gtttcttttt gcataaattt agcaacgacg
    16921 accccatttg accttgcaat cagcatggcg gcatatagga ggttgccgtt atgctgagta
    16981 aaatagcttt tgcttatatg gcaaaagaaa gtcttttgtc aaaaaaagtt tgtaccttac
    17041 aacgggatat tccgggcagt ggtatgaact gtcaaatcaa ttaaatactg ctgttatttc
    17101 ctatgcgtct gtctgcactt tgcaggcagg cgcattttgc attttacaga agatttttca
    17161 aaaactttgc tctctttttg gcaaagcaaa aagtcgtctg tattatcccg cgaaaagggg
    17221 aggtactacc gcctttcggc agaaaggagg tgaacaccat gacacctaat cgcagggaat
    17281 ttgaaaagca atgtgcgttt caaaagttct gtaaatcagt gctgcacaat gaagcgtgca
    17341 acgcccatga agaaattcgt cggcgcagag ttcgggaagt tgctttttct gaccttgcct
    17401 tgcatgagga gcggcagctt tataccgtcg ataagtattt tcaagacgaa gaagtagagc
    17461 cgtcttatca aatggcagga aaagaaataa ccccaaaact gctgcttgaa gctatccggg
    17521 ctttacccga agaacggcgc aaaattgtac tgctgtatta ctttgaggga ttaactgata
    17581 tagaaatcgg aaagcagctc aatctctcaa gaagtacggt acagtatcgc cggacaagct
    17641 cctttgagca gttaaaaaaa tatttggagg aaaacgctga tgaatggatt gaatggtaat
    17701 caaaacgact acccggaaaa tgcccttgtt ccctatcccg ttattttggc agcgagcaaa
    17761 ggcgacccgg acgcaatgaa gattgtattg cagcatttca gcggctacat agcaagtctt
    17821 tccatgcgga agctctatga tgagcgagga aatgtttatt ttggagtaga cgaagaaata
    17881 cgcgaaaggt tgcaagcaaa actaatgagg gcaatcctca catttagggc tgaataaacc
    17941 aacgcggtat tggaacgctt cccccttttc cgttccatat cgcaggacag ccctctgctc
    18001 tttgacaaag aaagcggcgc aatagaacgg cgacgcagta taaggcaaat cggatacgtt
    18061 ccttttgtgc cgagccatgc ggtgggtacg ccatgacctt tttcttaacc gaaaagcgag
    18121 cgagaaatac cacaccgtaa agcagggata gcaccctgcg ggcgataacg cacaaaatac
    18181 ataatgatac ttccttacag ccatagtccg agcgttagaa gcgtcgcagg caatgggtag
    18241 ggcttcggca gaccgccgga ggggtgcaag tcccatgagg ttacgctaat agccgtccga
    18301 ttaaagccta tgttacagat tgacttttga cccattaaga aagggggacg acaaattgaa
    18361 caataacgac gtgccgattt gggaaaagta cactcttacc atagaagaag cagcaaaata
    18421 tttccgtatc ggagaaagca agctgcggaa actggcagaa gaaaatcccg tcccggactg
    18481 ggtaatgatg aacggaaacc gtattcagat taagcgtaag cagtttgaaa aaatgattga
    18541 tacggtggac gcaatctgat agtgaaatcg agccttgaat gtggtacaat atagtcaagc
    18601 atatcaaggc tctttccatt atggaaagga gcataacgat gtcggaaaaa agacgggata
    18661 ataaaaatcg tattctccgc accggagaga gccagagaaa agacgggaga tatgcctaca
    18721 aatatataga tacctttggg aaaccgcagt ttgtttattc ttggaagtta gtgcctacgg
    18781 acaaaacccc ggcaggaaag cgcgacgata tagcgttgag ggaaaaagag aaagaaatcc
    18841 aaaaagacct tgacgacggt atcgaccata tcggaaagaa aatgacggtc tgccaactct
    18901 acgcaaagca gatacgccac cgggcaaatg tgcggcatgg tacaaagcag gggcgaaaac
    18961 agctcatgcg gattttgcag gaggataaac ttggagcctg ccggattgaa aatgtgaagc
    19021 tctccgacgc aaaggaatgg gcgttacgca tgaaagaaaa aggttacggc ttcaagacta
    19081 tcaacaacca caagcgttcc ttaaaagctg ccttttacac agccatacag gacgattgta
    19141 ttcgtaaaaa tccatttgac ttccagttga acacagtcct tgaagatgat acggaaccaa
    19201 aggaacctct atcccctacg caggaagcgg cttttctgtc ctttgtgcag catgataaag
    19261 tctatcagaa atactacgac gagattatca tactgttagg gacagggctt cgcatttcgg
    19321 aactctgcgg actgacggaa gctgacattg acttagacaa acagcttatc aatgtagacc
    19381 accaactttt gaagattgca gacgtgggct actatgtgga aacgcctaag acgaaaagcg
    19441 gcaaccgggt tattcctatg agtgaaaaag tgttggaagc atttcaacgg gtgctgaaca
    19501 aacggaaata tgcacagcct gttattttgg agggctacac aaagtttctg ttccttaacc
    19561 ggaacggctt gccaaaagta gcagtcaact atgaaagtat gttccggggg cttgtaagga
    19621 aatacaacaa aacgcaaaag gtagcattgc caaaggtaat gacaccgcac accttacgcc
    19681 acacattctg caccacgtta gcaaatgcag gaatgaaccc aaaggcattg cagtacatca
    19741 tgggacattc caacatcaat atgacgctga actattacgc gcatacgact tccgaaacgt
    19801 cgataacaga aatgaagcgg ctgattgcat aagtagtaaa cggagaattt actactccac
    19861 atactacttt tgaacaccaa aacatacggg gatataagaa gatttgagaa tatcttccat
    19921 aacgaaaaat gccggaaaag cctgtaacaa taggctatac cggcatataa gaacttttga
    19981 aaagatagtc aaaaattact ttgtgattta taatacatta gttatatatt ataatatctc
    20041 gacaatgtct taacaatatc tcgattatat attttaatat gtaactaata tgttataata
    20101 ttttaattaa attaaataaa tttatatttt tttgaaatta cttaatattt tatataaaaa
    20161 tgaaaaagca cctcattctg attaaaaatc tttctgagat actttttaaa atattaaata
    20221 ctacatatag cttacataag tttaaaattt aatctattat aaaattaccc taattgccaa
    20281 actttttaat ataaattttc caacatccta caaatataat caatagatat agaatcctaa
    20341 cttacaccaa tatatattta tttaaaatag tcctaaaata cctgtaatag ctcccactac
    20401 tatcagtata cacattattt ttattggaga aagacctttt tttaaagcaa agagtactaa
    20461 taaagtaagt accaaaggaa ttaacccagg aaatatctta tcaagtaaat ccgtttgaag
    20521 actaaattta aaatcaccac tagtataact tattggtgta gataaattaa taaacctacc
    20581 tacaagtccc cccatgacta aggtacctag tatagaagcc ccttctataa ccttatttac
    20641 tattccacct tgaagtatac tttcaacagc ttttttccca tacttatatc catttgtcca
    20701 cattccaaaa gaaattgaag tcattattat atattgagct attacaaata atataggtcc
    20761 tgcaattgat gctcctccct cagttaaacc aatacaaaca gccaacacta taggagttat
    20821 aactccttga gttaaagtat cgccaattcc cgctagtgga cccatcaagc ctgttttgat
    20881 agcattcata ccatctccac ttatttcatc tgctccatta gctttttctt cttccatagc
    20941 tattgttatt ccatgtataa cacatccaca tataggttct gtgttaaaaa atgctagatg
    21001 agtctccact tcttgtttta actcttcctt atctggatat aattttttaa gtactggcaa
    21061 cattgaatgt gaaaaagctg ttgattgcaa tctttcataa ttgtaattag attgtgcaaa
    21121 gaaaaaccat cttaaccatg attttattac atctttcttg cttaacgttt tcttagatac
    21181 ttcatttttt atatctgaca taatattttc cccctcacat gtatttaaga tgtagcacta
    21241 tcttttccgt acttaattga agtaattgta agagcaagta ttactccaat aacagatatt
    21301 actattatat ctactttaaa ataaacagcc attagtattc ctaatatgaa aaagggcata
    21361 gtaaacttgt ttccaatcaa ttttaaattc atagcaatcc caagtgctgg caacatagcc
    21421 ccaattacat taaatacatg taacacatta tcattcatta cttccaacat accatctatc
    21481 gcatttggac caaaataaac aactaataat gctggtataa aactcattgc aaataacagt
    21541 atctgtggtg gtactatatt tataaatgct actctatcaa cattcccttt ttgaacctct
    21601 ctatcagccc aatgtgcaaa aaaagaattt actgtcatct ttccaatcca aagtacagtt
    21661 gcaacagttc ccaatggtac agctattgcc attgcagtac ttacatctag cttagatgct
    21721 atagctaaag cagtacctac atatcctgca aaagctgggt cccccatctg agctccccct
    21781 gctgatataa aacctagata tatcatattt atacctgctc ctataattgc tccttgaaca
    21841 gggtctccta atataattcc cgttacaaat ccagccaata gaggtctatt ccatgtaaaa
    21901 aatccaacat aagtcaacca cggactccaa gaaagataat aaaaaattgc tattaatact
    21961 gcttgaatta gtgaaatgtg catattcttc ctccctattt tcttttaaag atttacaaga
    22021 tagtcttctt tataataatg ttcctacatc aattttcttt gcatctggta ctatttgtat
    22081 aaagcattta acatcttttg atattagctc tctaaatatt tctttttctt catctgacac
    22141 agatatattt ttataaaaac tttttctacc aagccctgct cccatacctc caacattcaa
    22201 ttcctttaaa tctaccccgc tatttactaa acctaagaca gttttagggt atttgaccaa
    22261 tactattaaa ttttcatctc cattatgtga ttttaagact tcagcacctg attctacatc
    22321 atgtacttca accttaacag ttgatggagc tgccatttgt aaaacttgaa ctataaatgg
    22381 gtcctttact actaaatcat ccacgattac tatccttttt gctgatgtaa tcttacacca
    22441 agcagtaata acttgcccat gtattaatct atcatcaatt cttgttaatg ctatattact
    22501 cataattatt cctccagatt tttaatttat tttacaactt taaaacagtt ttaacatctt
    22561 tgattcctga tatcccaact tcctcacata aattcttcaa gtcttttaac ttcatagtat
    22621 ctctgtttaa aaataactct aaaagcatag gtaaatttac acctgtcaaa cattctaact
    22681 cacatacctt agcaaattta tttattataa tagcagatgt attaaatgga gttccgccat
    22741 aaaaatcaag caatactaaa accccttcat ccttatattt ctcaattcca tcttctaact
    22801 ttctactaaa ctcatctatg ctgtcaccat ggtttaatcc taaagtttct acattttcct
    22861 gtttgcctac tattaattca gcactttcta acattgcttt agccatattt ccatgtgtgg
    22921 ctataattat tcctgccata tgaatcatac ctttcttaac cttttttata tggattatta
    22981 tttagtactc taatctaatt atctatatta atcattatct taccaaagaa aatatccctt
    23041 ttgtatactt tttcaaaata agctggtact tcttctaaat ttattctatg tgttactaat
    23101 ttttcaacat tcatgtcacc cttttgcata tgatacaatc cagcactcca ttcttttcct
    23161 gggaatggaa aggagtttcc aaaccaagtt ccctttacag ttaattcact tcttactatc
    23221 ttttcaaatt gctctctagt caaagctaca tccccatatg gtacacctgc atacaatact
    23281 gtacctccct ttttagctag caacagtact tgaccacatg tcagtggagt accagccgat
    23341 tcaataacaa tatctgcacc atctccatca gttaaccttt ttatttcttc tacaatattc
    23401 ttttcttttg catttataca aatatctgcc cctaactctt ttgccaagtc taacttctca
    23461 tcaaatacat ccacagctat tatttttgta gaaccaaata tttttgccca ttgtattgaa
    23521 aataatccta ttggacctgt accaagtact acaacagtat ctccaacttt agcttctgac
    23581 ctaaaaagac catgtcctgc tatacatact ggttctagcg cagcggcagt ctcataactt
    23641 atctcatctg gtatttttat taaatttctc tctttcacct ttgtatactc tgcaaaacaa
    23701 cctccaagtt ctttatttcc tattattgct acattattac atcttgaata caagcctttt
    23761 ttacattcat cacattcaaa acatggcata gctggacaaa cagcaactct atctcctatt
    23821 ttaaaactcc ttacttcttt accaacctgt gccacttctc cagaaaattc atgccctaaa
    23881 atttctccaa ccatatgtgg acctgtcttt gaatattttg atatatcaga accacatatt
    23941 ccagctactt ttactttaat tattacatca tctttttcta atatttttgg tacatccaca
    24001 tcctccactc ttgtgtctct tataccatag aatctaactg acttcactat taaccacccc
    24061 tactttttat ttgagaatat atccctattg tttatatgtt aaatatctaa agcaactttc
    24121 atgccaatat atgtaagcat tgtattcatg cattttataa aattatcatt aaatgtgtgt
    24181 caaagtgtgt caaaagtttc ttatattaaa acttatgtgt gtcaatataa acgacactaa
    24241 cattttatat tagttctctt attataagtt ttataaaatg tacttttttt gttattttta
    24301 taaaattgtt cggaaatttc ccttctattt gtgatataat attaaatata aggtatcaat
    24361 ataaattttc atgcgaatat cagaactttt ttgacactaa tgccttattc aatataataa
    24421 tttaactttt tggaggttgt ttatgaaaaa agtgtatcaa ggaaagacta aagatgtttt
    24481 tgaattagat aatggaaatt acttacttaa atttaaagac gatgtaactg gtaaagatgg
    24541 agtatttgac ccaggcgaaa atagtgtagg tctaagtata gatggaattg gtagagctaa
    24601 tttagagaca tctgttaagt tctttgaaat tctaaacaat gctggaatta aaactcatta
    24661 tgtaagtgct aatttagaag aagcaactat ggaagttcta ccagcaaaag tttttggtaa
    24721 tggattagaa gttatttgtc gttatagagc tgttggtagt ttcttacgtc gttatggttc
    24781 atgtgttgaa gaaggtgcta aactagattg ttacgtagaa gcaactttaa aagatgatga
    24841 ccgttgtgac cctcttataa catctgaagg tttagaagct ttaaacatta tgactcaagc
    24901 tcaatttgat tcaatgaaaa gcatgactca aaaaatttct aacatagttc gcgatactat
    24961 agctgaaaaa ggtcttgagc tttatgatat taaatttgaa tttggattct acaatgatga
    25021 agtaatctta atagatgaaa tagcatctgg aaatatgcgt gtttataaag atggtgtaat
    25081 agttgaccct atggatttaa ctgctctttt attagataaa tagtatatac atataattta
    25141 caaatataat aaacttataa ataagataag ctatgaagta aagacttttt aaatgttgat
    25201 tatttttaaa cattttctcg cctttaattt catagcttat ttaaattaat atactctaat
    25261 tagtagataa tagctttaac cctacaattc ccaccacaat aaaacctata catgcaattc
    25321 ttgatatact aataggctca tttaaaatag ccatgcctac aatagctgta ccgacggtac
    25381 caattccagt ccatattgca tatgaagttc caagtggaag atgttttagt gctaatgaca
    25441 aaaaataaaa acttacaatc attccaacaa cagtaagtat acttggagtt aatttagaaa
    25501 atccatgtga attttttagt tgaaaagccc aaaacacttc aaacaaacct gcaacaaaca
    25561 aaaataacca tttcataaat tcatccctct tttcaaatat gtctcttatt tttccaataa
    25621 aataacctga gaaatattat aaaatatctc ccaggttttt gtccttccgt gttcacaaca
    25681 aaaactatgt tgtgggtttt atctcggacc agaccaatca ttaataattg cggaacccta
    25741 taaaactcta ttaattattc tattttatct ataataatac tatcaacata tataaaaaaa
    25801 gtcaatatat gggttccata tataataaaa ataactttta tatacctttt aatattctta
    25861 ttcacttctc tccataccca tttgggtcaa tctaagaata tctaattttt atttaatatg
    25921 ttataataac ataaaaatat tgaaaggagt aatttctatg aatttctata aaattgcagt
    25981 atgtcagatg attactacag aaaataaaat tgaaaatatc aatcatgctg tagatatggt
    26041 aacagaagct gccatcaatg gatctaaaat tgtagttttg ccagaaatgt ttaactgtcc
    26101 atatgaaaat aaatattttc ctaaatttgc tgaagaatat ccaggtgaaa caacaacaat
    26161 attgagtaaa ttagctgaga aacatggaat ttatctagtt tctggtagta ttcctgagtt
    26221 agaagatgga aaaatctata atacttgcta tgtatttgat aaaaatggag ctctaattgg
    26281 aaaacatcgt aaaatgcact tatttgacat tgaagttaca ggaaaagtta gttttaaaga
    26341 atcagatact cttacagctg gaaatgatgt cactgtaata gataccgaat atggaaaagt
    26401 tggtatagct atttgctatg atattcgttt ccctgagtta tcacgtttaa tggctcttaa
    26461 aggtgctgaa atcgttatct tacctgctgc atttaatatg actactggtc ccgcccattg
    26521 ggagttatct ataagaatgc gtgctttaga taatcagata ttctatgttg gtgctgcacc
    26581 tgctagaaat atgaatgctt cttatatagc ttttggaaat tcaagaattt cagacccttg
    26641 gggaagaata attgctcaag ctgatgaaaa agaatgtatt atttatgctg acattgatag
    26701 agatttaatt cctgacataa gacaacagtt accacttctt aagcacagaa gaactgattt
    26761 atatgaactt aatactttga aataaaacag aacataaccc cataaatcgt aaattaagtg
    26821 ttaaaaaaat ttatggggtt ctcattaatt tgatttaaaa ttaatttcat aaaaataatg
    26881 ctaaaattaa tttaattaaa taatactaat aattactaat gattatgcta attatcagca
    26941 tttactaatt taatcactat tagttatttc ttttattaaa tatctttact tttttactta
    27001 ttatgtcttc tagctttaca tttggattat ggtatttctt tctattttta gtcacatctt
    27061 tctattgaat tacttgctta aaacaccaat gcacacatgc cagataatgt tcacaattat
    27121 gtagaaatct tgctctacct tcttcaatgt tgatattatc tctcctagtt gatattatct
    27181 ttcctaaaat ttcttttaat taaaaaacaa cattagttga tatatcaagt atattttaat
    27241 caactgaaaa aaacaataat aattgatata tcaactaatt aatgtagatt aattcatttt
    27301 tataatttat atacttttta attaatctac cttatttaat taaaatcctt cctttctatt
    27361 tatccatttt caacttaaat tataagattt catcagctat atttttgcat accaataagt
    27421 attaaatatc aaatatatat tctattaaaa tttctaataa tttcatcctt gatttattta
    27481 atatataaaa atatactaac tttaatgatt aagttttgaa atataactaa aaatttgata
    27541 atttattgag atttttatac taaaatatac atactataca aacttaattt agaagggagc
    27601 attcttatga acaataaaat actcgtcgta gatgatgagg aaggtataat aacactctta
    27661 aaagattact ttgaaatgaa tggttatgag gtttacactg ctcaaagtgg aaaagacgca
    27721 ttaaaaaaaa tatccaaaaa tccagatatc attctacttg atataaacat gcctgaaata
    27781 gatggaattg aagtttgtaa tagaattaga gattatgtat catgccctat tctatttctt
    27841 acagctagaa ttgaaaacac tgacaagata aatggttttc gtgctggtgc tgatgattat
    27901 attgtaaaac cttttgattt agacgaactt ggagctagag tatctgccca tttaagacgt
    27961 gaacaaagga agtcaaatca aactaaaatt aaattttata atgatttaac aatagattac
    28021 gaaaaaaggt taatttgtat atataataat cagatttcac tttcaaaaaa ggaatttgat
    28081 atagtagagc ttctctctat gaatcctgga caagtatttg ataaagaaaa aatatatgat
    28141 agagtctggg gatacaatag caatggaaat agtgatgtta taatggaaca cattagaaaa
    28201 atacgaatca aactatcaaa acatactctt gaaaattata tagaaacagt ctggggagtt
    28261 ggttataaat ggataaaata aataatatgt cattaaaaaa agctttcttt gttcttactt
    28321 tatcttcact aattatagca tcagtactta ctactttcgc atacatttta cttaataatc
    28381 tatataattc tatacaagat aagtatttaa aaacacctat aaatacaggt attataacca
    28441 tagttcaaaa caatgtgaac caatttagtg attatgataa aacattactt aatataatca
    28501 acatactcca gcttgtactt ccacttatat tttttatagg acttttatta ttagctgata
    28561 taatttttta caaggtaaaa ttaaaaacac ccatagaaat actcaataaa ggtgcaatgg
    28621 aaatatccaa caacaattta gacttttgct tagaatataa taataatgat gaattaggta
    28681 atctatgtaa tgcttttgaa aaaatgaggt ctgaactaaa taaaaacaac ataaagatgt
    28741 ggactatgat taatgataga aaacaactta atgctgcttt ttctcatgat ttaagaaatc
    28801 ccctaacagt tctaaaaggt tactcaaact atttaacaaa atacatacca acaggtaagc
    28861 ttagtgatga gaagatttta tcaaccacac aacttatgtc tgagcatatt aacagaattg
    28921 aatactatgt agaaaatatg agtaatgctc aacgattaga agatttagtt gtttcaaaat
    28981 ctatgtcgaa tataaacaag tttatagaaa acttagatga aaatatatct atcattgcta
    29041 aacaagaagg aaaatctttt acacttaaaa atcaaataaa taatataaat ctattttttg
    29101 atgaaaatat cattcttaga gtcgtcgaaa atataatatc gaatgctttt agatatgcta
    29161 gaaataatgt atccatactt atatatcttg aacaagaact ccttaccttt gtaattgaag
    29221 atgatggtat aggatttagt aaggaatctc taaaattagc tttaagacct ttctatagag
    29281 ataagacttt aaatgacagc aatgcacact ttggtatggg tctttatatc tcaaaaatat
    29341 tatgtgaaaa acatggtggc tctatttcta ttgaaaataa ttctactaac agtgctaaaa
    29401 ttactgctaa attctcaaca agaaattaaa atgattttgt aagctttcac tgctattata
    29461 taaaatttgt tagtttagaa tttataaaat gtatttcaat ttataaccaa tatatatact
    29521 attttgtaga aattgtaaga gttgcttcaa ttataatatt ttggcaaaag gcatttgaaa
    29581 caataataga aatatatatt taaatatgta gaatagtatg aaattagaat tttggaggtg
    29641 attatattaa aagttttgct aataatatta cattctctcg tattgtgttt gcattgctaa
    29701 tgcttagttt tacattctgt tcactgcctt tctggatatg ttatttttgg tgtggaatta
    29761 gtgatatact tgatggtctt attgcccgaa agttgagaca acaaagtgga ataggtgcaa
    29821 agttagatag tattgctgat ttcatttttt cggttacaat tttggttact gtcggaaaaa
    29881 atattgctct tcccatgtat ctatggtggt gtattattgc aatagcattg ttatggttga
    29941 ttagttacag tattggtttt tttaagtatc gcacattttc atcgcttcat actttgatga
    30001 ataaaatcac tggaatttct atctttgcgt ttccattact ctatatttgc tttggtcttg
    30061 atgttgcagg aattatgata tgtatcgtgg cttttctttc ttcttttgaa gaaatagcaa
    30121 ttatgataaa atcaaaagaa ttaaatcgtg atataaaaag tatatttatg tgaaaactta
    30181 gaatttgtga caaaaataat ttatataata atgaggaagt aaaacataaa aaattatcca
    30241 caagtttatg tgtaaaatag ttttaaagca atagagttgt attgtaaagc ctttagtgca
    30301 taaattacat tttaaattaa aaatgatact gaaactgctt atcagcactg cgaattatcc
    30361 gcaaatagtg aagacttttt agatttaagc taaaagttgg gaaaaaattg agttttaaaa
    30421 cttacactct tcttatatag aaaaggagag tgttttttat gattataaaa gcaaaacaat
    30481 tatcaaaaat atatggttct aataacaata aagttatcgc acttaataat gtaaatttag
    30541 aaataaactc aggtgaattt gtttctgtaa ttggaccttc tggaagtgga aaaagcacac
    30601 ttttacatat tttaagtgga ttagataatc ctacctctgg acaagtgtta cttgatgata
    30661 aagatatata taaacacact gaaaaagaac tatctgcact tagaagaaaa tcctttggat
    30721 tcgtctttca acaatttaat cttttacctg ttcttacagc atctgaaaat atttcaatgc
    30781 ctgtacttct agataaaaaa caacctgata aaggctattt aaatgaaatt tcatcattac
    30841 ttggaattgc agatagactc aatcatcttc cccatgaact ttcaggcggt cagcaacaaa
    30901 gagttgcaat tgctagagca cttattgcaa aaccagatat aatatttgct gatgagccta
    30961 caggaaactt ggacagtaaa agtggcagtg aagttatgaa tcttcttata aaaacttcaa
    31021 aacagtttgg aaagacttta gtggtaatta ctcatgatga tagaattgca aaacttgctg
    31081 atagaaaaat ttcaattatt gatggtgttc ttatggaggt gaaatagaaa tgaagaatta
    31141 cctttcactt tcaataaaag aattaaaaag ccagaaactt atgactacat tcatcattat
    31201 agcaatagta ctatcaacta ttatgacaac tgtggttgga cagtcaattg gtatcctaca
    31261 aaatttaaga attgaacaag cccgttcttt taatggtgat agacatgtat cctttcacca
    31321 actcactaaa aatcaagttg atgacataaa gaaagatgat agagtttatc aagctggtac
    31381 ttcaataact attggcagta gtaaaataaa ggattctggt atctctgtac ttgtaaaaga
    31441 gtatgataaa acaggacttt ctaactaccc aaaattaatg aaattaaaat ctggacattt
    31501 gcctaaagat aaaaacgaaa tagccttaga tgaaaacaca ctaaagctta tgggtataaa
    31561 accaagacta ggagtgacta tacctatgaa tttagatatt tcacttttaa atgataccat
    31621 accaccatat aattacacag ctaactttaa attgagtggc attttagagg atgattatac
    31681 aggatatgta agtggtatag taaatggtat tgtaggtaaa ggtacatcag aaaatctatt
    31741 acctaagaga tatactttat cctctttgga ttttaaaata aaacagcaag aaaagtttca
    31801 agaaatagta aatcaattag ctaaaaaaat aaatctttct cacaattcaa ttcaatataa
    31861 ttggatttac ttaaatgctc ttaagataca gtttgaaaaa gatgaaaata gttctaatag
    31921 tgatggtata tctatgatta tattggtatc tttatttgtt gccttattgg ttttactggc
    31981 ttctggactt gtaatctata atattttaaa aatctctgtt acaaagaaaa ttaaagagta
    32041 tggatgttta agagcaatag gagctgaacc aaaccagata tataagatag ttattttaca
    32101 aatcttaatt ttatgtactg ttgccatacc tattggagct gtaattggaa ttatatcttc
    32161 gaaaggcata actggaatgg tcactaatat cttaaatcca gatatacttc tagcaaatga
    32221 taataaagaa attactgagc ttatacataa aaatacaaca gcatatatgt tcccattagt
    32281 attaagcact aatgtatctt tgattttttc acttatttca gcattaccat cagcaatata
    32341 tgcttcacat gtctctccca aaattgctat ggctggtagt actaccaaaa tcaaaagaaa
    32401 aattaaaaga gaaaaaacaa taaaaaactt tgaaagacat ctagcatggc taaacttaaa
    32461 aagaaataaa ggaagaacta taataactat actatcatta tttatgagta taacagtatt
    32521 cgttgcactg agtgagttct ctaatgtatt agatgtatct aggtcagtaa gtaatttaaa
    32581 agaaggagat ttttccttaa caaatgaaat atctggtttt gataaatcaa cactagataa
    32641 aatagctaaa atgaaaaacg tcaatcgcac ttcctttata aagtactctg aatacaaaca
    32701 gggtgaaata gatacagata taaattttga aaatagtggt gagatgttaa aaatcattgg
    32761 aattgatgag caaactttaa aagacttaat gccttctatt acaaattcta tactagaaga
    32821 ctttaaaaat ggttctatct gttttataaa aaatccgatt gctatttcta caccaggtgt
    32881 aaaaactaga tatacaaatt taaaacctaa agatagtatc acaatcaaca aaaaacaact
    32941 agatatatat agtacagtag ataaaatgtt ctttttacaa ggcaatggct ggataaatgg
    33001 tgttgatgtt attgtatatg attctgttta taacactttg acaaataaaa acaagttcaa
    33061 ccagataaat atttatgcta aagataaatc aaaattagaa caaataagat tgtctattga
    33121 acagatttgt gaaaataatc ctggctctca ttggatatca tatattgaat cagataaaca
    33181 acttaaagag agttttaaac aaatagaatt tctagcatgg gcagttatat tatttgtggg
    33241 attgattggt actttaaaca tcattaacac aactcataca aatataaata ctaggactaa
    33301 tgagattgga gttaaaagag ctattggtat gagtaatagg agcttatata aaatgttttt
    33361 atgggaaggt gtttattatg gaatttttgc tgctatattt ggttcaattg ctgggtatgc
    33421 cagtgctatt attataaaca tggcaactat agaaaagctt gatttcacta atataccaat
    33481 tacttcaatt ttacaagcta ctattatttc tgtacttaca tgtattatag ctactcttat
    33541 tccgttaaga aaagttaaaa agatgaatat aattgactgt atagatatca atttataact
    33601 attttacaga taataatata aattaaatta tcaatgactg tctctatttt ctttcaaatc
    33661 taactaataa atctttttaa ctataatata agctttaaaa aaggcctgtc tcaaaataga
    33721 gataagcctt ttttcattgt cgaaataatg aaaatatatt taacttcaca aaaaaagagt
    33781 gcctcacgaa ttaaaaattt cgctttgaga cactctcctt taatttaaaa tatatagtta
    33841 aatgctatgc caaaatttca cacatgctcc acctgatttt gaattttcta ttttaacaga
    33901 acctccaagc tgttccacta attgtttgac aatgtaaagc ccaagaccag aatgtccacc
    33961 ttttgtctgc ctagcttcat ctcctcgata aaatttatca agagcctttt ttaaatcttt
    34021 agagctaaac cctctaccag aatcacaaat ctcataagaa atacaatttt tttcatcctt
    34081 tacagtaata ctaatattac cacctgatgg agtgtattgc agactgttgg atatgatatt
    34141 atcaaaaatt cgtgtcaacc tatccacgtc gatttgaatt ggggattgta tattcccctg
    34201 cattttcaat ataagttcaa cttccttttg atgagcctgt aattcatagt cttgcacctt
    34261 ttgcctcaaa aattcaggta aatttatagg aacaagattt aattgtatat ttgacttttc
    34321 taagtcagac gtatattgca tcttttggac aagagcagca cttttttctg tattttctct
    34381 tatcactgta agatacctat gaagtttttc attgtcatct gtattattac caataagtgc
    34441 atctgtatat cccaaaataa tagaaagagg agattttaaa tcatgtgcta gagcttccac
    34501 catttcaaca cgttcctgct ccatcttcca ctgtgcagat aaagagcctt ttaattcgtc
    34561 tttcatttca gaaaatgcac tacaaagttt accgagttca ttatctgaat aatagtcgat
    34621 ttcaaaatca aggtcctttt cttttatctt cttggacgca tcaatcaata gatgtaatgg
    34681 ttgattgata ttctttacaa agcgcttaga aagccatcta gtaaaaccca cgatatatag
    34741 aaatggagaa aacagagcca ttataataac tgcaaataca aatctccctc taatatttgc
    34801 aaaggtgatt ttcacttggt agaatagaat aacagctccc tcaatctttc cattatcccc
    34861 tttgattgga acagtatgga tataaccacc tttacgaaca gttttattga taaaattatt
    34921 aaataattct tcttttgttt taaatagttt tttggggtta gtaccatata aaatattgcc
    34981 attattatcc acaacttgat atagcatatc atccccacga atcgttcttt ttaaaccttc
    35041 ttcattcgat tgtgataaca aagttatgtt tttttcattg atatattttt caattctagg
    35101 aacttgtcgc tcataatagt ttggtggata gatatcctta tttaaggaat gaatatacaa
    35161 atagagagca aatacataag taatcactgt ggcaactata ctggctatta taataaaaat
    35221 aaaagtatgt ctaaattggg taataaggga ttgatttttc atttaataac ctctctctct
    35281 ttacatttta ttccacttat acccaatacc ccaaactgtg gaaatatatt ctttttctgg
    35341 tgtcacagca gaaaacttag ctcgtatttt ttttactcgc tcaacaacag ttgaggaatc
    35401 accttcagaa tcacaccccc atactttttc atagatttgt tctcttgaga aaacttgtcc
    35461 tgcatggagt gctagtaatt caacaatatc atactctatt tttgttaaat caatattctt
    35521 accatcaatt ttaattgcac gttccttcat atataagcat aacttcccaa aatacaaagt
    35581 agatctcttg ttctcttcat tgatatactg tgaacgcttt tccctacgca gattagcttc
    35641 aatccttgcc ataagttcac gcagtccaaa tggctttgtt acataatcat cccctccaaa
    35701 ggtcaaccct ttgattttgt cggtttctga ttgctttgca cttaaaaata taattgggca
    35761 taaaacatca tctctaattg cctgacatac ctcaaatcca ttcattttag gcatcataat
    35821 atccagtata ataaggtcag gctcctgttt tgataactct atacctgcct gaccatcata
    35881 agctatcaac acttcatgtc cttttgcctt caattcatcc tcaagtagca ttactaagtc
    35941 catttcatca tcaataatca atattttact caatataaca ccacctttct cttatgggaa
    36001 ggtcagtatt aaaacaatat tcgtctacta ataacttttc cttccctccc acattttaaa
    36061 ccaaataacg cctcctatca agaatataat caaacctatt gctactacta taagctctat
    36121 actagctgtt tgcatcactt tagagtaaaa tgcctcagtt gaaatagttc cagcttcttt
    36181 aataaaataa attcctggaa gcatacctag ttttactggc catgtccatg gaacaaatgc
    36241 ccatactttt tcaccaacgc tagttaatcc aagtattgct gccatcaaaa ttccaccgat
    36301 actaatacca atagatgcac ccatacccca tgcaaaactc acccaaagat gaatagctaa
    36361 tagtggaagt gtcccaatca ccacaagtcc tgctgctgac aggaataacc atattgttgc
    36421 tccagatggc actgcaatac tcattcctat gcaaagtatc aacgtagcaa taaatgtgca
    36481 tatagttagg caaaacagaa gaaaaagaaa ttttcccaga tataatctaa cacgagaaat
    36541 acctacacat aaaaaaccat taaaatttcc agcaagctct tcctcctgca ctataaatcc
    36601 tgctaaaata cctactccaa taggaatgat gaatacagtc catactgtaa aaaatccttc
    36661 aaaagcaaat tcttgtgtac ttccagattt agttgtgaga taaaaaacca cacataatga
    36721 aaaaataata ggcataaaaa gagtaagcca ctggacagct gtcctctttg ctcgaagcca
    36781 ttctgatgat aatagcttta tcatttgtca tccctcctat tgaaccaagc tgcagtaagt
    36841 gctgttaata ctataaacac aaccaaagaa acaacaatcc caataggaat cactgaagta
    36901 ttcaaaagtg ggtctccagc ttctaatata gtaccgttag gatgtatgtt gatgattggg
    36961 cataccattc taactgccca actccaaggt actgcaatcc aaaatggttt tgtcgcagca
    37021 aggacaccaa atatcatacc taaagctcca actcccatac ttaggaacat acctttccat
    37081 gtagctagcc atagctgaag tggaagtata gcaagagaaa caacccagca tacaatactt
    37141 gcccctataa tctgtccaaa aggaactgga ccagctttca atattaaacc agttataatt
    37201 gttacgacta ttaaaactag cgttgaaata aaactgtaga ctgccatagc tataacttta
    37261 ttaatccaaa gtgtcatagg agacacattg tgtgtacgta aagcacgata atttccagct
    37321 tttttttcct ggaaagctac caaagtagca aacaatgcaa atccaatagg cagaaataaa
    37381 aatgaccacc agttaaaaat taatgctaaa agactttgcc acgcccaaga agttgtttga
    37441 gataatggat tttgcatgag tgttgattgc attataaatg catatgctgc aaaagacact
    37501 gggaaaaaaa caataagttt acccataaat gttcttttat actttaaaag ttctgattgt
    37561 aaagcattca tctgttttgt cctcctttaa gaataacatc tgtaaaaaat tcttctagat
    37621 tttcattagg gtcaggcatt ccttgaaata atagtcgtcc tccatttata attcctatat
    37681 tatctacaac ttgagcaacc tctgatagaa tatggctaga aagaattaca gttatacctt
    37741 tttttggaaa agaagcaatc aattcacgca attcttgaat gccaaagggg tcaagaccat
    37801 tggttggttc atctagtatt aacaactctg ggtcactcaa aagtgcaata gctattccta
    37861 acctttgttt catacccata gaaaattgtg aagctctctt tttacctgta tttttcaaat
    37921 ttactatttc caacacttca taaattttat ctttaggtat tcccaatatt tttgtatgta
    37981 ctaacaaatt ttcctctgct gtaaggtttc catacaatgc aggggattct attaaagaac
    38041 ctatttttgc aagagattct cgtttccaag gctttctatc aaaaataata cgaccatttg
    38101 ttggacgaag aagaccaaca agcattttaa gtgttgtgga ctttcctgct ccatttggtc
    38161 ctaaaagtcc atatatacta tttcgttcaa tttgtagaga aacattgtta actgccattt
    38221 gttctccata tctttttgat aagttttttg tttcaagaat taaatctttc atattaattt
    38281 ctcccttctt gtaatacctt aaatctgtta ctaagagaat actaacatga aaaaataaca
    38341 attctataac acctactaat tttaataaaa tttaaaataa aaaatagagt taccaatttt
    38401 attttggcaa ctctatttaa gtttatgttt tacagttata ttatatttaa acttagtttc
    38461 attatcacac taacttcaaa tttactctat aatctttaaa tcaaacacta atttattaat
    38521 caaacactaa tctattaatt tatatttctt tagacatcta atatagtttg ctactaaata
    38581 ataattattt atatcatatt tatctaatcc tttaaaagaa gttatttcat cattaagctc
    38641 atcactacct tctacaaaaa cacaacctgt ttcaaattcc atacctacaa taatcttaca
    38701 gccactataa atttcctctt gagtatcgta caattttttt gcatttcctt catttggaat
    38761 tgtaaataca ataggtttta attctttatc actggaaatc ttttgtgaaa aaggcttgtt
    38821 taacttaata ttattgataa ctacatgttt aaattcctct gaagtttctt ctactattac
    38881 atattttcgc tttaaaaact ctattatttc taaaacagac ttagaattta cttttaaaat
    38941 acttttatat ttttcatata aagatttcca ctgttcaatt ttttcctgtg gtatttcttg
    39001 gatatgtgta cttatttttt cttgagatat ttttttcaat taaactctcc tttcaatact
    39061 taaatattct tatgaatctc acatgactaa agttacaagg tttctactta atagaatttt
    39121 gcatacttaa atatgtctta ttaattctct acaggatatc tccgtagttc ctacgattct
    39181 tacataagtt acttatttta aattgttatt agttagatta taaaaattat ttattttatc
    39241 actaatataa tactcttaaa aactttacaa atcaatttaa atttgctacc ttttatttat
    39301 aacaacttat agtacatttc tcctatgctt ttcaatatat tacttttaaa aggaggtttt
    39361 cattatgatt ttaaatagag ctgagctttt ccgaggatat tctttaaata gatttaacga
    39421 gatatgcaat atattatcag taaataaaat taaatataag tataaagtga aaaataatgc
    39481 ttactctaaa aaaccttttt ttaatgaaat aacacttggc aatttaggcc aaaaggaaga
    39541 tttctcttat gagtacttaa tttatgttca taaagacgat tatgaacagg cagagtctct
    39601 aataaattct aagttgcgat agattatcta taaaacatga cattctgttg tcatgtttta
    39661 tatgttatta tactttcaaa tatgctacta tattttcaaa attgtgtgct ttaaaactag
    39721 ctttttttag taatttaaag cttaaaacta aggagactcg ttatatgaaa ataaatataa
    39781 aatcaaagtt aagtgaattt attaaacaga acaatctttt tgatgacagc agaataatat
    39841 cctatcttcc taatataaca atagaaacag acaaaataaa aactataatg attaatgagg
    39901 ttgtcccaac taataccaat gacgattttt atagtgtaga taaagatgct gattatttaa
    39961 aaactactat tcctttattt gacagtgcag gaataaaagt atctaacata aatgatatcc
    40021 tagatatggg tatctatata actaatgcag taaaacttcc taaatcagaa tatacaataa
    40081 ctcgtgatac aattaaatta catatgccta ttttagaaga agaaattaaa ttatttaaaa
    40141 acctagaggt agtaatgtta atgggagatg ttgcaaaaaa atcttttaat atgattacta
    40201 aaaagcatat aaaaaagaat gtcatccctt ccatttcaac atataaactt cgtaataatg
    40261 aactttatta tgataattta agagtatttc catcatatat tatgactggt ggaaacatac
    40321 tgattgaaaa atcaaaattt gaaatggcat cagaagatat aaaaaaaatg ttcgagatta
    40381 taaacggttg atattacaaa ataataaaaa ctaatataaa ataaaaagtg cctcaaaaac
    40441 ttctatttat gaggcacttt tattttatat tagtgtaact atatactatt aacataattt
    40501 attatttata ttgttctttt taatctctat tccatttagt ctaggtaaca ttactcttta
    40561 caagattact ttatatttct tttatatctc taacgttttt atttaataaa acaattatta
    40621 atacaagtca attatatcta tttatctaaa aatatatctt ttacagtaat atctggatgg
    40681 tgatacttct tcctattcgt taaaaaagat ttttttacat taatagcctc ttttggacac
    40741 cagtgaaaac aagctaggca tctttcacac tcattacccc actttggctt tttatcaact
    40801 atacttatat tctctacagg acaaaccctt ttacatgtcc cacatccatt acatttattt
    40861 tctacaaaaa atttagtagt tgcaatttgc atattcttac ctactaaacg aagtacaaaa
    40921 ctatctatat gtgactttaa attgtttttt ccttctatat ttccaatctc caagttattg
    40981 atacattttg caatctcagc taccctatta gtctgatttt tcaatctttc ttcttcaaca
    41041 tctggaggtg ttatcaaagc atttccaggc atattgatta aaaaaccagc agataatttt
    41101 ttcccccttt ttttaagtaa cttatctata gtaaataaac tatgtccagc taccccatta
    41161 caagtggcaa tggcatatat atgagtatta atattaaaat ttaatttctc tacaaaagac
    41221 tttactatat caggaacatc agcaaaataa acaggaaaaa caattaccaa tatatcttca
    41281 tttatgtcta tattattttc ttttctatgt atagctaaag gtataacttc tttacttcca
    41341 tttaacgttt cttttaaaat ctttgaaact actagcgaat ttcctgttcc agaaaaataa
    41401 taaatcttac tattcattct tatccctctt tctatatttc tacatcatca atagttaaaa
    41461 agtctcttaa aataggattt atatttactt tccaattcta tccaaaatat cagtaaacaa
    41521 aggcaattct tcatcagaga tagatttaaa tgtctctgtt gcatatatac ttgcaatttc
    41581 atttaatggt tcttctaatt ctctacctaa atctgttagg aatatttgaa acttacgtct
    41641 atctttaaca tctactttac ggacaattaa tcccttattt ttcataacat ctaagattct
    41701 tgtcgtatta gatttatcct tacatgattt ttcacataaa tctatctgag aaatgccatc
    41761 ttgctcccat agaaacctta ataacgcata atggtcagtt gttatatcat atccattttg
    41821 tttaaacatc ttttttaaat catcttttac ttttaacgaa gtacttacta acttagttgt
    41881 agcttcatat agtttctcaa aatctttcat tttacctcca tttaaataat cccttgctcc
    41941 tcccatctat caattattcc cttatattgc tcaatatctt taaaagccat tggctttaaa
    42001 acagcattta tagcaataaa attatagatt tgagaaagca caggtggatt tttcttttcc
    42061 tctatgcaat aaataaattt tttagatacc ttttgtatct ttgattctac tttttcagtt
    42121 ataggatttt gattaaatcc agtttttata gataaactac cacaaacata acctcccgaa
    42181 tctaagacca tggattttat aaaagaaatt gcttttgaac cacgtgcatt ttcatatgta
    42241 gtaactgcta tacatggctt acgatacaat aatctttcaa gcatcatatg tgctctatct
    42301 gtaaacacct tgaaaagtcc agttacacta cttccataag ttgggctacc aaatataaca
    42361 ccatcactct tagtaattat atcatgaata tactctactt tatcattttg attgatacac
    42421 ttgcccattt tgtaacaatt taaacaacca atacaatatg atggatttac ctcacttaaa
    42481 tcaaaataat taatttttac atctggtatt aatctttcta tatctttata caagtaagtt
    42541 aaaacttttg atgtagctcc attttttcta gggcttccat ttataactgt aattatcatt
    42601 tctatatctc ctttttagtt gttatatcaa caataagtat atcaactata tttttagctg
    42661 tcaatataaa ccatttattt aaagccatac ttacacagct taaataaaag ttttatttaa
    42721 ctacatgtgc ttatttaatc aactataaat gaattatttt taataattga caaactaaat
    42781 taatgataat atactttatg tattacaaat gtaagtagaa ggaggtatag tatgaaaaag
    42841 ttaccacata tttcagaagc agaatatcaa gttatgaaaa ttatttggaa atatgctcct
    42901 atcagcacta ctgaagtaat tgaaaagttg gtagaaacaa gtacatggag tcctaaaaca
    42961 attcaaacaa tgttattgag attagttaaa aaaggagctt taacatatga aaaaaatagc
    43021 agagtattcg tttatactcc acttgttaaa gaagaagaat acgtagctag agaaagtagt
    43081 tcttttttaa atcgttttta taatggtact cttaattcta tggtactaaa ctttttagaa
    43141 aatgataaac tatctgaaga tgatattgag gaactgagag aaatcttaaa taaaaggacg
    43201 actaaggggg ataaataaat atgtatgccc cattctttat acgattttta ctcagtacat
    43261 taaccttatc aatattaatt gctgtaattt tacttgccaa aaaagtattt aaaaaacata
    43321 tttctgtaaa gtaccaatac aatattggat ttatattgct tataatgtta ataattccat
    43381 ttgtccc
//
LOCUS       DAEMIK010000012        42876 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEMIK010000012 DAEMIK010000000
VERSION     DAEMIK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.012"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(172..360)
                     /locus_tag="KPK87_000569"
     CDS             complement(172..360)
                     /locus_tag="KPK87_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536762.1"
                     /translation="MTKTILCDYCNKGMNKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHGYSKK"
     gene            618..761
                     /locus_tag="KPK87_000570"
     CDS             618..761
                     /locus_tag="KPK87_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536763.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     regulatory      complement(964..1059)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1335..2216
                     /locus_tag="KPK87_000571"
                     /pseudo
     CDS             1335..2216
                     /locus_tag="KPK87_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2221..3939
                     /locus_tag="KPK87_000572"
     CDS             2221..3939
                     /locus_tag="KPK87_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2536764.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWSYDL
                     RLTLEHFSMKNSIDGIKPFINSIKISLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(4019..5173)
                     /locus_tag="KPK87_000573"
     CDS             complement(4019..5173)
                     /locus_tag="KPK87_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2536765.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            5672..7033
                     /locus_tag="KPK87_000574"
     CDS             5672..7033
                     /locus_tag="KPK87_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2536766.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            7124..7828
                     /locus_tag="KPK87_000575"
     CDS             7124..7828
                     /locus_tag="KPK87_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2536767.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            8027..8338
                     /locus_tag="KPK87_000576"
     CDS             8027..8338
                     /locus_tag="KPK87_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2536768.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            8360..9673
                     /locus_tag="KPK87_000577"
     CDS             8360..9673
                     /locus_tag="KPK87_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2536769.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            9750..11060
                     /locus_tag="KPK87_000578"
     CDS             9750..11060
                     /locus_tag="KPK87_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2536770.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            11086..11817
                     /gene="chbG"
                     /locus_tag="KPK87_000579"
     CDS             11086..11817
                     /gene="chbG"
                     /locus_tag="KPK87_000579"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG2536771.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            11830..12147
                     /locus_tag="KPK87_000580"
     CDS             11830..12147
                     /locus_tag="KPK87_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2536772.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(12259..12723)
                     /locus_tag="KPK87_000581"
     CDS             complement(12259..12723)
                     /locus_tag="KPK87_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536773.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            12993..13931
                     /locus_tag="KPK87_000582"
     CDS             12993..13931
                     /locus_tag="KPK87_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2536774.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            14059..15072
                     /locus_tag="KPK87_000583"
     CDS             14059..15072
                     /locus_tag="KPK87_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2536775.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            15078..16100
                     /locus_tag="KPK87_000584"
     CDS             15078..16100
                     /locus_tag="KPK87_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2536776.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMNVIK
                     TFLWQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIVVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            16115..16897
                     /locus_tag="KPK87_000585"
     CDS             16115..16897
                     /locus_tag="KPK87_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536777.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            16909..18249
                     /locus_tag="KPK87_000586"
     CDS             16909..18249
                     /locus_tag="KPK87_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2536778.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(18344..20623)
                     /locus_tag="KPK87_000587"
     CDS             complement(18344..20623)
                     /locus_tag="KPK87_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2536779.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20960..22123)
                     /locus_tag="KPK87_000588"
     CDS             complement(20960..22123)
                     /locus_tag="KPK87_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2536780.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(22136..22411)
                     /locus_tag="KPK87_000589"
     CDS             complement(22136..22411)
                     /locus_tag="KPK87_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2536781.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            complement(22432..23406)
                     /locus_tag="KPK87_000590"
     CDS             complement(22432..23406)
                     /locus_tag="KPK87_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG2536782.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVK"
     gene            complement(23677..25590)
                     /locus_tag="KPK87_000591"
     CDS             complement(23677..25590)
                     /locus_tag="KPK87_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2536783.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASKK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            complement(25767..26981)
                     /locus_tag="KPK87_000592"
     CDS             complement(25767..26981)
                     /locus_tag="KPK87_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG2536784.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            27225..28607
                     /locus_tag="KPK87_000593"
     CDS             27225..28607
                     /locus_tag="KPK87_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG2536785.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            28619..29968
                     /locus_tag="KPK87_000594"
     CDS             28619..29968
                     /locus_tag="KPK87_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG2536786.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            30118..30651
                     /locus_tag="KPK87_000595"
     CDS             30118..30651
                     /locus_tag="KPK87_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2536787.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINHYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            30735..31544
                     /locus_tag="KPK87_000596"
     CDS             30735..31544
                     /locus_tag="KPK87_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2536788.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            31954..33999
                     /locus_tag="KPK87_000597"
     CDS             31954..33999
                     /locus_tag="KPK87_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2536789.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKKNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            34260..35459
                     /locus_tag="KPK87_000598"
     CDS             34260..35459
                     /locus_tag="KPK87_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2536790.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            35511..36206
                     /locus_tag="KPK87_000599"
     CDS             35511..36206
                     /locus_tag="KPK87_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG2536791.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            36223..36900
                     /locus_tag="KPK87_000600"
     CDS             36223..36900
                     /locus_tag="KPK87_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536792.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            37011..38612
                     /locus_tag="KPK87_000601"
     CDS             37011..38612
                     /locus_tag="KPK87_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2536793.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNIN
                     KEIDCCGKVMTPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRTPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            39061..40350
                     /locus_tag="KPK87_000602"
     CDS             39061..40350
                     /locus_tag="KPK87_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG2536794.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            40379..41059
                     /locus_tag="KPK87_000603"
     CDS             40379..41059
                     /locus_tag="KPK87_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG2536795.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            41160..41699
                     /locus_tag="KPK87_000604"
     CDS             41160..41699
                     /locus_tag="KPK87_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG2536796.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            41875..>42876
                     /locus_tag="KPK87_000605"
     CDS             41875..>42876
                     /locus_tag="KPK87_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2536797.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     "
BASE COUNT        15814 a         5098 c         7191 g        14773 t
ORIGIN      
        1 aatatataaa catcaataac attactcaac acaccttaaa attgatttaa ataactcttt
       61 ttcatgcaca aaattaatga tataataaaa aagtaaatat gtaaccccaa cgcatattta
      121 ctgagtcaaa cattattata tagagcattc ttcgttgtgg agagtgcttt ttcatttctt
      181 tgaataacca tgttgattat ttataatatt ttcatttaat ttatctttat ctatcaaatt
      241 taatgcacaa ttaatacaaa tgttagtttt catatgactt ttcttatgaa aagtaatata
      301 cttattatca tctttattca ttcctttatt acagtaatca cataatatag tcttagtcat
      361 atttttattc ctttcatttt atattttcca taattaagtt taacatattt agtatatacc
      421 tatgtatata tatttaaaac aatgtttttt attgcaatat agaatatatt gtataaataa
      481 aaaaaatgga gaatagatat ttgataattg taaaattatg ttataattaa aatgcaagaa
      541 taatcttgtg gaactacaat ctaagagtgg agcttcattt tctacattcc atcccttaaa
      601 aggaaggagg tggagaaatg agcgaatttt tactaggagt gttagctagt ttaacagcta
      661 gctttattac atatattatt tccagaaaag taaaaagcca ctctggcagg agtgactttg
      721 agcttgatgt aaaaatcaag tttaataaaa aacgacatta atatttagtt aatgaaactt
      781 cactctaggc taatagattg tagtttcttt ttttgttttt gatacgaatt tacatcttta
      841 ttatatcgca ttttaagaaa aaataaaact atgaatatta gaaatatatt ttattttatc
      901 tctaagtata tatattatta gccacctaaa aaactttcat aaaaacaaaa agaacaatat
      961 ctttttggca actggctggc ataatttata agaattttat aaatcttagt ccctatagct
     1021 ttgcgtcact aattttctct agttttgccg atttagtttt gttctacaac taaaataata
     1081 caatagaatt agttaatgtt caacaaggtt gtttgaaaat taaataaatt tataggtatg
     1141 aatagtattt attcctaaaa aggaacagag tatttaaagg tcaaggtaag ttagaatagt
     1201 attgtgaact aacatttgat tatgcgaaat tctatgaaac aggaaccctg tgactttagt
     1261 catgggaggt tcagtgtata tcgcgtattt caattgttat aagtatttta tttatttgga
     1321 ctaactaata atagccaagt ggatgtggta aaactacatt acttagaata atagcaggtc
     1381 ttgaagatgt aaatagtgga actataattc tccaggataa agatattaca aatttagagc
     1441 catcaaagag aggttttggt atagtatttc aatcatatgc attatttccg aatatgactg
     1501 cttataacaa tatagctttt ccactaaaag agagaaaagt ttcaaaagaa aaaatagata
     1561 ataaagttaa ggaagtactt gaaacagttg gattgactaa tgaagctcat aaatacccta
     1621 aagcattatc aggtggacaa caacagagga ttgcaatagc aagagcatta gctcttgaac
     1681 caaagttttt acttttagat gaaccaatgt ctgcattaga tgcaaaggtt agacacaagc
     1741 ttagaatgga cattaaaaga ttacaaaaag aacttaatat aacgacaata atggtaacac
     1801 atgaccaaga agaagctata actatggcag ataaaatagc tattctaaat ggaggagata
     1861 taatgcaaat agggactcca gaagaaatat accaaaatcc tcaaaaccta tttacagctc
     1921 aatttatagg agatacaaac tgttttgata atggagattc tatattaact gtaagaccag
     1981 aatatgttca aatagagaaa tcgacaaaag aaaactacca agggataata tcaaatatag
     2041 agtttagagg gaatttattg agagttgaaa taaaagataa attaaatgaa aatttcataa
     2101 taagtgatgt ttctataaaa gagtgggtta atttaaattt agtagaaggt gaccttgtaa
     2161 aaataagtat agacgaaaaa tactatttaa agtatccaaa agttaaagga gcataagtgt
     2221 atgaataata atgctataag ttctaaaagt ataagtctta aatcaaaaat gaatttagat
     2281 ataaaaaaat gtatgcttac agtattagtt atgtctttag taatattttt attagcacct
     2341 ataatatatc tatttgtaca ggctttttta gataaagatg gagtctttgt aggattacaa
     2401 aactttcaag aatacttggc ttctgaatct ctattaaatt ctctaaaaaa tacagtattt
     2461 gtatcagtta tatcaactgt aatatcacta acacttgcat tagtatatgc atatgcatta
     2521 actagaacaa agattaaatt taaaggtttt tttaaatcat tagctatgtt tcctatgttt
     2581 gctccaacaa tgatgcatgc aataggtcta gtatatttat ttggaaatca aggtttggta
     2641 actaatttac ttggagtgca tgttccaata tatggagcag tgggaataat attggcacaa
     2701 attatatatg tatttcctca atcatttcaa atactttata tatctctaag ttcaaccgat
     2761 tatagactat atgaagctag taatactatg gggataagca agataagaga atttataagc
     2821 ataacaatcc caagtataaa atactcttta ataagttctg cattcgtatc gtttacatta
     2881 tgttttacag attttggagc tccaaaggtg ataggaggag agtttaatgt attatcaata
     2941 gatgtatata agcaaattat agggcaacag aatatgccaa tgggagcaac tgtatcaata
     3001 ttacttttaa tacctgcaat aatatcgttt atagtaacta aaaaaattga aaaaaatcaa
     3061 aattcatata taacttcaaa gtcaattcca tataaggtta aaactaataa gttaagagat
     3121 agtattttag gaggggttgt aggaattatt gcatttggta taatagctgt aatgttaact
     3181 gtaatcttat cttcattaat atcagtttgg tcatatgatt taagactgac actggaacat
     3241 ttttctatga aaaacagtat tgatgggatt aaaccattta taaattcaat aaaaatatca
     3301 ctattgacag ctattctagg gacgagcttt gttttcttat ttgcatattt gaatgaaaaa
     3361 acagaaaaag ctccatattt aaaatcattt gggtattttt taagtacatt gcctatggca
     3421 ttgccaggat tagttttagg tatagcttat gtattatttt ttaataaatt agagtttaat
     3481 tttatggata caatatacat taaaaatttg tttaatggat tatatggaac attacttata
     3541 atggttatat gtaatatagt tcatttcttt tcagtagcat ttataacagc tactactgct
     3601 atcaagaaag tagataaaga attggatatg gtagctaaat caatgggaat aaacagcgca
     3661 tcaattctta agaacgtaac tataccactt tgtttgccag cagttttaga gaactttatg
     3721 tactttttcc taaactccat ggtgacaata tcagcattag tttttatata tactgcaagt
     3781 tcaaaagtag ctgcaatatc aattgtacaa ctagatgata aggggagtac tgcacaggca
     3841 gcagcactag ctgtgttaat attagtcaca aatatattag tgaaagtaat atatgaattt
     3901 ataaagaatc gtttagaaaa aagaacaaac tttaattaaa taatttatct tataaaacac
     3961 aaatttaaaa agtctattct tatgatttat tatttaaaga atagactttt taaatttatt
     4021 attgatattg tttattaatt ttccttttgt tatcatacat ttgagaatct gcacgacgat
     4081 atgtatcaaa taaagaaaca tctacttttt catcaaaaat agcatatcca atagacattg
     4141 ataattttaa ttcattagtt gagttttctt caactatttt taaattaata ttatttataa
     4201 tattatttat ttctttgtga gataaatttt ttagaataat tatgtattca tcacctccaa
     4261 tacgataaac tatatatttt ttatttatta catattgtat aattttactt gcttgcttaa
     4321 tatataagtc tcctgtctga tggccaaatt tatcattaat cttttttaaa ccatctaaat
     4381 ctattgatat gatagatatt agattcttta gatttgaatt gtttaagtta ttcatatcta
     4441 tttcaaaagc atttctattt tttaggcctg ttaaataatc tgtattagca aaatcagtat
     4501 atgtaggatt agatattctt ttaaatagaa tcactgcaat caatgctgat attagacaag
     4561 ttaataatgc tattattggt gtaactatac ttataaattt aaatgtattg tattgatgtt
     4621 ttgcttcaaa ttcaattcct attactccaa caaccttacc aacatcattg tgaataggaa
     4681 aatacgatat aaatatgtat ccccaagatg ttgatttaat ctttttaggt aacaatatat
     4741 gattatctaa tgctttgtta aggtctcccc aaatctcttt ttctatcaaa tcaccagcat
     4801 ttctaaaatc actcctagat gaatttaaac catcaacaac atatactagt gatccatctt
     4861 ttgttttttt agcagtatat agatacatga ctccagtagt attttttaca gattctaaaa
     4921 ggattttaga acttttataa gacttttttg attggtcttc tttagaatta atttcagtaa
     4981 aggtattttt atcaatattt ctttctacat attcatagat atgttttgac ctctcactta
     5041 aagagtatat catgctctta taagttagtg tataattaaa aaaatataca catgaggaag
     5101 atattattac aataattgca gtaagtattg aaacctgcat atcaactcta ttgaattttt
     5161 tatttatttt catagcttaa atgtcttgtg aaaagaacct cccacaatct ccccataatt
     5221 aatatatatc tataaatttc accataatat attcatatat tatcaaattt gtgtggaaat
     5281 tacaattagt aaacatgctt aaagcattca aatatagttt ttttattatt atagtatact
     5341 attttatgta taaataaaat tatatataaa tcattgatat tggttgttta ttaatggaag
     5401 ataccgtata aaatattcta attggttacg atatgtatac ttaattgaag ttaagttaag
     5461 ggttagaata tgtattgatg aaatggctag atttatattc ataagtatta attttatcat
     5521 agataggtaa aatactgtat cttaaacata agtatgattg agatgcaatc ctgagatatg
     5581 ataatataaa ttgaaggata gtgaactcaa tcaaataaaa tgattaatct agctatataa
     5641 gcaaataaat ttaatataaa agaggtggaa aatgaaagac agacttaatt taacagaagg
     5701 aagaattaca gaaaaattac ttaagctatc acttccaata atggggactt catttataca
     5761 gatggggtat aacatgatag atatgatatg ggttggtaaa gccggaagta aagcagtagc
     5821 tgcagttgga acagctggtt tttttccatg gcttgcaatg gcatttatta tgatttcaaa
     5881 agtaggtgga gagataaaag tagcacaaag tattggtgca aataatataa gcactactaa
     5941 atcatatatg aagtcagcaa tagaaataaa tattatacta gcaataatat atacaatttc
     6001 tttaatagta cttaataaac aattgatagg tttttttaga ttaggagact tagaagttat
     6061 aaccatgtct agacagtatt tgataatagt agctctaggt atggtatttt attttattaa
     6121 tccagtattt actgcaattt tcaatggtct tggaaatagt aaaactcctt ttcgtattaa
     6181 tacagtagga ttaataacta atattatact tgaccctata ttaatttttg gatggggatt
     6241 tgctccaaaa cttggtgttg caggagctgc tattgcaact gtatttgctc agatagtagt
     6301 tactacatgt tttatatata taatagttaa aagtaaagaa gaatatttta aattaagact
     6361 atttaaagaa atagagacta aatattataa agttctgtat aaattgggat tacctatagc
     6421 aatacaaagt ggtatgttta cagtattctc aatgctatta ggagtaatag tagcatcatg
     6481 ggggcctgtg gctgttgcag tacaaaaagt aggctcacaa atagaagcaa tttcctggat
     6541 gacggctgaa gggctatcag ttgcattagg cagttttgtt ggacaaaatt atggtgccaa
     6601 gaagtattct agaataaata aaggttgcaa aatagctata actgtatctt ctgtacttgg
     6661 tataataact actttagtat tagtgtttgc aggagagagt atattttcta tttttataga
     6721 tgaaagtgag gcaatagaaa aaggagcagt ttatcttaaa atattaggct attctcaatt
     6781 ttttatgtgt ttagaaataa taacaacagg agctttcaaa ggattaggga gaacctatat
     6841 accttctatt ataattacta tactgactgg tgcaagagta ccattggctt actttatatc
     6901 tagaccagaa atgttgggtc taaatggtgt atggtggagt ataaccttat caagtatatt
     6961 aaaaggtata ttaatgataa gtctatttat atctttatta aaactcggaa agttgtacaa
     7021 gatttctgaa taaaatttaa attatcttaa atgtatagac attttctttg tttgtatata
     7081 aaattatctt attatataaa agatttaata aggagtgaat taaatgaaag agccaattta
     7141 taaagttata gaaaatcatg ttagagagtt aataaattca gacagtttga aagaagggga
     7201 tttaatccct tctgaaaaac agctgagtga agaatttaat gtaactagaa tgactgtaag
     7261 gtctgcactt aataatctag ttaaagaagg ctatataact agacaaagag gtgtaggtag
     7321 tatagttatt gcaaataata tatatgataa tatatcgtct gtaagtggat ttactaaaga
     7381 gatggaaagt aaaggctata aagtatctaa tatattggta tctcttgaaa tagttcaggc
     7441 agatgaaaat ttatcaagta agttgaatat aagtttagaa gaaaatgttt gggaaataaa
     7501 aagagtaaga ttagctaatg atgcaagagt ttcatatatg attacttata tgccagttaa
     7561 gttatttcca aatttaaata agactcattg tgaaaattct ctttataatt ttgttgaaga
     7621 agtgtgtgga tacaagatag ccatgtcaga gagagaagta caagctgtaa tatcgaatga
     7681 agaatgtatg gataatctaa aattagaaga accagagcca cttttatata taagccaaat
     7741 ttgtaagtta caaaatagtg agatttttga gtactcacac acatatcact atggatatac
     7801 attaactttg aatgctgttg tcgaataaaa tttaaataaa atgaaaaagt tttcttgaaa
     7861 tgtatataca tttggtatat aataatatca agaattaaat gtatatacat ttaaaaataa
     7921 gatatataca ttaagaaaaa attaatagaa aagcatattt taaatgtata gtttaaaaat
     7981 ttatgactta aaaaatattt aatgaatcta atttggaggg gaaaaaatga taaaaataat
     8041 gttagcttgt tcagcaggaa tgtcaactag tttattagtt acaaaaatgg aggatgcagc
     8101 aaaggaaaac ggtatagaag caaaaatatg ggctatatca gaagtgaact taaaaaatga
     8161 aatagaaaat tgtgatgtct tacttttagg gcctcaagtt agatatgtat taggaaaagc
     8221 aactgaaatg gcaaaaccat ataatattcc agtagaagtt ataaatatga tggattatgg
     8281 aagatgtaat ggaaaagctg tgttagatag agctgtagag ttaaatagtt caaaataaag
     8341 ttgtactgga ggatgtaaaa tggaaaaatt catgtcattt atggataaat atatagtgcc
     8401 tgtagctgct aaaataggag ctcaaaggca cttagtagca gtaagagatg catttatagt
     8461 aatgatacct ataactatgg taggtgcatt gggaacattg ataaataact taccattgga
     8521 agcatacaaa aatttgatgg caagtatatt tggagaaaac tggaccacat ttggaggcga
     8581 cctttggtgg ggagcaatag gtacaatggc agtattctta gtaataggtg ttgcttattt
     8641 tctagcaaaa tcttatgaga gtgatgggtt acaatctggg cttatagctt tatctatctt
     8701 ttttataatg gctccacaaa ttggtaaaat agttcctgag ggaggaacta cagtagttga
     8761 aggatgggga atgatacaac aaacctactt aggtacagca gctttattct cttctatatt
     8821 aataggatta ttatcaactg agatatttgt aagattatct aaggtaaaga aattaactat
     8881 aaaaatgcca gatggagttc caccagcagt ttcaagatca tttgcaaagt taataccagg
     8941 aatgttaact ataatgatat ttactgtaat aggaatattt ataaaaatgc tttccaatgg
     9001 aagtttctta acagatattt taaatactta tttaggagca ccactatcaa atgtagctga
     9061 tagtttaggt tctacaatgc ttattgcatt tataattcat atactttgga ctgttggact
     9121 tcatggtgca aatatagcac ttccttttac agaaactata cttatgaaat taggtggaga
     9181 aaatgctgcc ttggctcaag caggagcaac ggaaggatac catgtacttg caggtgcatt
     9241 ctttgatgca tttgtatatt taggtggttc tggaatggta ttgggattaa tagtagcttt
     9301 attaatagct ggaagaagac gtaaagagat gatagtatta ggtggtccac ctgcaatatt
     9361 taacatagga gaaccattga tatttggact tccaatagtt ttaaatccta tatttatgat
     9421 tccatttgta cttgcaccag taatatgtag tgctgtatca tacttagcaa tagattttgg
     9481 attagtagca ccagtaattt tacctaaaat accttgggta acacctccta tattaggtgg
     9541 agcaatggct acaggtgact ggactggcgg agctttagca ctatttaatt taatattatc
     9601 aatattaata tatattccat ttgtcatagc gtctgaaaaa atggaagcta ataaattgaa
     9661 aattaacaac taggtagttt tatttcggta aataaatgta tagagttttg tagagtatat
     9721 taaattatag gtaaggatgg tataatgaga tgaaaaaggg actaaaaata gttacaattg
     9781 gaggaggttc aagttatact cctgagcttg tagagggttt tataaataga tatgaagagt
     9841 tacctgtaaa ggaattatgg cttgtagata tagaagctgg aaaagaaaaa ttagagatag
     9901 ttggaaatct agctaagagg atggtaaaaa aagctggagt agatatgaaa ataaatctta
     9961 ctttagatag aagagaagct ctaagagatg cagactttgt gacaacacaa cttagagtag
    10021 gtcttttaga tgcgagaata aaagatgaaa ctattccttt atctcatgga gttatggggc
    10081 aggaaactaa tggtgctgga ggtttattta aagcccttcg cacaatacca gttatatttg
    10141 atataataaa agatgtggaa gaattatgtc caaatgcatg gatggtgaat tttacaaatc
    10201 caacaggaat aataacagag gctgtattca aatatactaa ttttagaaga tatataggtc
    10261 tatgcaacgt gccaattcat ttaaaaaatg atgttgccaa actatttaat gtagaaagtg
    10321 acagaataag tatggatttt gcaggtctta atcatatggt ttatggtcta aatgtatctt
    10381 tagatggaga agatgttaca aaagaagcaa ttgataagtt tgtcaaagca gatataagta
    10441 tgcaaaatat aaaggcaatt gattttaatg ctgaatttgt aaaatctttg ggtgcaatac
    10501 cttgtccata ccatagatac tactataaaa ctaaagaaat gcttgaggat gagttgagag
    10561 agtttaaaga gggaaaagct agaggtcaag tagttaaaga attggaagaa caattatttg
    10621 aattatataa ggatgaaaaa ttagatgtaa aaccacctca gcttgaaaaa agaggagggg
    10681 cttactatag tgatgctgct tgtaatttaa taagttctat atacaatgac aaaaaagata
    10741 tccaagtagt taatacatta aataagggtt ctataagaga ttttaaagat gaacaagcag
    10801 tagaagtaag tagtgtaata acaaaaaatg gtccaaaacc aataagtata ggatatttac
    10861 cagattctgt gcatggatta gtaagtcaaa tcaaatcatt tgaagtattg gcagctaaag
    10921 cagctgtata tggcgattat gaatctgctc ttttggcact ttgtataaac ccattgattc
    10981 catctgatga cttagcaaaa actatattag acgaaatgtt ggaagctcat aaagattatc
    11041 ttcctagatt taatagatag ttgtctatag aaagtaggtt atggtatgac taaaattata
    11101 ataaatgcag atgattttgg atattgtgaa gctgtgaact atggaataat ttcagctcat
    11161 aataatggta tagttaggtc tacttctatg atggcaaata tgcctggggt agaacatgga
    11221 gtaggcttac ttaaagaaaa taaaacttta aattgtggag ttcatatgac tttaagttgt
    11281 ggtagaccat tattatctaa cttaaaaact attgttgata aagacggttt ttttattagg
    11341 aggattacag acgaaattat tgaaaaaatg gattgtgatg agatttatag agaactttgt
    11401 gctcaaatag atagggtaaa agggcttgga atagatatat ctcatttaga tagtcaccat
    11461 catatccata ctttagtaag tttaaagcca gtaatagaga agattgtaac caagtataat
    11521 cttccaataa gaggtggatt tgaatataat ttagagtatt ctaaagtagt tcctttaata
    11581 gatagttttt ataaggaaaa tgtaagtgaa gaatatttta taaaaaatat agacgaaata
    11641 atgaagtatg atgtagtgga tattatgtct cacccagctt ttttagatga ttatatttta
    11701 aattctacat cttatgctat tgatagaacg aaagagcaca agatacttac atctaaaaaa
    11761 gtcaaagaat ttttagaaaa gaatggatta gtcatatcaa gctatagaga tatttaggag
    11821 gtcgaataaa tggatgagaa gatgatagag atatcttttg gaataattgg atttgcaggt
    11881 gatgcgaaag ggctagcatt tgaagcaata aaagaagcaa agagcgggaa tatagataaa
    11941 gctagagaat gtattgaaaa gtcgaaagaa tcaattcata aagctcataa attccaaaca
    12001 gaattgatac aaaatgaagc taatggtaat aaaactgaaa taagtgttat acttattcat
    12061 gcacaagacc atttaatgac agctatgaat tttcaacaat tagcagaaga atttatagat
    12121 gtatatgaaa gattagaaag taagtaattt aaaattatta ttaaaaaaga gtgtataaaa
    12181 ataattttta taattattaa atgcactctt tttttattta agtagagttt agcaatatag
    12241 tattatgtta ttgatacttt aaaatttaga cttctcataa ttgtaaggta tactagtacc
    12301 aacaactaca tcttcaattt tttcaacaat aagccaatca tccatatttc cttgatggtc
    12361 aaaatcaaga ggtgttattt cttcaacatt ttcaaattca actaagcata aacatttttt
    12421 atgccaacga actttttgct tttcagataa atttaatttt ggttgatttt catttaaaat
    12481 tttagaaatt tcatcttctg ttaatttaac atagttttct acctttttaa cagtggcttt
    12541 tgcagatatt tttgcagttc ctttttccat aaaataaagt aactcatctt caaatactct
    12601 actatgtggt atttttctac cagcagcacc tctaataacc attgtttttg ttccatccaa
    12661 gattttatct aagacctttt ctcctttttt tcctgcatta tcacaatata ctaaatgtac
    12721 catgttttga atcctcccta ctaaattcct tatctataaa ttgcatttta tcattgttta
    12781 actgaaaagt cattgtattt gtgtccaatt cttaaaaaaa atactgttta atataagaaa
    12841 accttatcta agctatatat aattaagatt aattaggaat ttttatcctt ttacaaaata
    12901 atattgacta aaaatagaaa aatgctataa taaatatgtg cttatgataa tcaatatcat
    12961 gtaagtataa taaacaagta ggagaagtaa tcatgaaaaa aataaaatca cttgcaatat
    13021 ttatatctat tataactacg ttagtacttg ttacagcatg ttcagataag aatacagagg
    13081 ataaagataa atcagagaca agagttgtac aatctgttaa aggagaggtt aagataccat
    13141 caaatcctaa aaaaatagtt gatatatcag gaagtagtga agaactatta ttagcaggtt
    13201 ataagcctgt tgcaacagca aatgtagact catatgaaac tgataaatta ccaagttaca
    13261 taagagaaga gcttaaaggt gtaaagatag ttgggcactc aatgatggat actatggata
    13321 tggaagctat attagaagta aatcctgatt taataataat gagtcaaaga caagaaaaaa
    13381 tatatgacca attaaaagaa atagctcctg tagttatgat gaaagattat gcaaatgact
    13441 ggagaagcaa gttaactgat gtttcaaagt tatttgacaa agaagaagaa gctaaaagtt
    13501 ggctacagaa gtatgatgaa aaagctacaa agttaggtaa ggaagttata gaaaagaatg
    13561 gagaaaaaac ttatttacca gtgttggcaa gttcaggtca atttatggta tttagtgatg
    13621 gtggaatagg tactctaatc aatgatgata tgaaattagc tagacctaag aatatgccaa
    13681 aacaggatgg aataacacta ccaatggtaa gtatggaagg tttaacagat atagatgcag
    13741 accatatagt tgtaattgct acagaagcag ataaaaaaga tttagaaaat agtgctatct
    13801 gggctcaaat aagagcagta aaagatggta atgtaacaat acttgatgct gctccatttt
    13861 tcagtcaatc atacaatcca attggaaaag agttattatt agaatcagtt aaaaatgaat
    13921 taacaaagta attcttaact tagtaggtgt gagataatga tattcatttt tcacacctat
    13981 ttttaataaa tcttaaaatt aaaatactta atagatgata ttatactgac gattaaaagg
    14041 gaaaaggagg taggataatt gactaagctg agtaaaaaaa atataactta tttgttgata
    14101 ttagtaagta tagttgtact tgtatttgga attgtattat ccataactat tggggcaaaa
    14161 gatatgaatc tatcaactgt tatagatagt ttgataaaaa tggaagatgg aataaatatg
    14221 agaatagtaa aggatgttag acttccaagg gcaatagcag ctgcacttgt tggagggttt
    14281 ttagcagttt ctggggcaat aatgcaagga ataacgagaa atcctatagc tgaaccatct
    14341 gttataggaa ttacacaagg agctacattc gcaatttcga tatcactggt acttcaaaaa
    14401 aaacttcctc aacttataca tggaagtttt agtgtaatga tgtttgcatt tattggagca
    14461 agtataagtg gattatttat atattttata agttctaaat caagaggaag agttaataat
    14521 gtaaaattag ctcttgcagg agtcgctctt ggtacattgc taatttcttt ggcatcagca
    14581 atatctatgt attttaacct atctcaacaa ttaagtttct ggatatcagg agggcttgta
    14641 ggagtgaagt gggaaggtat aaaattgcta tttgtagctg gtggtattgg atttatttta
    14701 gctattatta tggctccaag aataactata ttaagtcttg gagaagaagt ggcaatagga
    14761 ttaggacaaa aaactaactt tgttagattt atatgtatag tacttgtaat attaatgact
    14821 ggagcatctg tgtcagtgtc tggtaatatt atttttatag gtcttatagt tcctcaaata
    14881 gctaaaggaa tagtaggttc agattataag tatataatac cgagctcttt agtattagga
    14941 gctgtattgc ttgtatatac agatatatta agtaggatga taaatcctcc atatgagaca
    15001 ccagtaggtt ctataacagc acttatagga gtgccaatat ttatatacct tgttagaaag
    15061 ggggagaaat aggtactatg aaaaatatta taaaaataaa ttctaaaaca ttacttgtta
    15121 tatcaatttt aatggtgctt atttttatca cttttttatt gagtttaaat atgggctcat
    15181 tttctataga accaatgaat gttataaaga cttttttatg gcagggtcag cgtaatcatg
    15241 aaattgcaat atttaaatta agattaccaa gaatagtaat agcacttttg gttggttctg
    15301 cattatcaac agcaggtgta atacttcaag gagttacaaa gaatgattta gctgattctg
    15361 gaattttggg aataaattca ggtgctgcat tgtttgtagt agtttatata tatcttatga
    15421 atggtaatgt atatgaaggt atgagtaact tgactgtatt tacaatgcct attgttgcac
    15481 ttataggagc tttatttgga gcatttctta tatattctct tgcatggaaa ggtggtataa
    15541 attcgtcaag attactactt attggtatag gtgtaaacat agcttttact tcaattttga
    15601 cgatattcca attgaaattt acaacacaag aatttaatag ggttatggca tggacatcag
    15661 gaagcatttg gggaaccagt tggaaatatg taatagttgt gcttccattt atagtgattt
    15721 ttatgggtat aactatttat aaatctaaat atatagatgt tttaaatttg ggtgatgaag
    15781 tatctataag tcttggtgta gatgtagaaa aacaaagaag aagacttata atatatgcag
    15841 taattttatc tggtgtatcg acagcagtag caggtagtat ttcattttta ggtttaatag
    15901 ctccacatat aggtagaaaa ataataggac caaagcataa aagattaata ccagtatcag
    15961 cattgatagg aacattatta cttttaatag cagatactat atcaagaaat cttctagcac
    16021 ctatagaaat accagtaggt atagtagttt ctataatagg agtgccgtac tttatttatt
    16081 taatgttggt aaatgattag gaaagggtgt tgttatgaac tgtataaata ctaagaatct
    16141 aaatgtatca tatggaaaca cagatattat aaaaaattta aatttaaata taccaaaagg
    16201 aaaaataact accatcatag gggctaatgg ttgtggaaaa tcgacaatac ttaaaactat
    16261 aggtagaatt attagcccta atagtgggaa aatatatata aatggagagg acatctacaa
    16321 aaagaagtct aaagaaatag caaaggatat ggctgtactt ccacaaagcc ctcaagctcc
    16381 tagtggtctt accgtttcag aattaatttc ttatggaaga tttcctcata aaagtggatt
    16441 tggtaattct agtaaagaag acagagagat tgtaaattgg agtttagaag ttactggaat
    16501 tgcagaattt aaggatagaa atatggaaga tttatcagga ggacaaagac aaagggcatg
    16561 gatagctatg gctttggcac aagaaactga tatattatta ctggatgagc ctacaacata
    16621 tttagatttg gcacatcaat tggagatact aaaattatta gatgaactaa ataaaaaaca
    16681 aggaagaact attgtcatgg taatacatga acttaataat gcagcaagat ttgctgacca
    16741 catgataggt attaagaaag gtaaaatagt ttgtgaaggt gatgcatatg atgttatgac
    16801 gaaggataat ctaaaagaat tatttaatat tgatgcagaa atagcaacag acccaagaac
    16861 aaataaacca atttgtataa cttatgacat ggtgtaaatg gtgacaacat ggagaatcaa
    16921 gaagcattaa gacatgaaaa aatatggatt ttattattta ggtattcgat acctgcaata
    16981 atagcaatga tggtaacttc actttataat gtagtagata gagcttttat tggttctatg
    17041 gaaggtattg gctctatagc tattgcaggt cttggtgtaa ctatgccagt atttactcta
    17101 ataatagctt ttggaatgtt ggtatcagta ggtgctagca caagattgtc tataaagtta
    17161 ggtgaaagaa atagagaaga agcagagaag atattaggta atgctttgac attatctata
    17221 attatctctt taattataac tatattagga ttagttttct tagaagatat actttttatt
    17281 ttgggtgcaa gtaaagattc aatattttat gcaaaagact atatgagtgt catattggtt
    17341 ggaagtatat ttaatttagt tgcattttct cttaataatg caattagggc agaaggtaat
    17401 ccaaaattag cagcaaggac aatgattgta ggatgtgtat taaacctgat attggaccct
    17461 atatttatat ttgtatttaa tttaggtata aaaggagcag ctatagcaac agtactctgt
    17521 caaatagtag tattcttttg ggtaatacat tattttataa gagggaagtc caatttaaaa
    17581 ctaaaaaaat gtaacttaaa attgaataaa gatatagtaa agaaggtatt tgcaatagga
    17641 atgacacctt ttgctatgga agtagctata agtataactc acatgtttac aaataattct
    17701 ctaaaggttt atggtggaga tttagctatt ggagctatga cagcacttac atctatttta
    17761 ttgatgttta tgatgcctgt gtttggattg aatcaaggta tgcaaactat aatatcatac
    17821 aattatggtg caaaacaata tgagcgtgca aagaaaactt taatattatc tatcatagtg
    17881 tcagttgtca tattgtcatt tggattttta gtagtacaag ttttcccaga agtatttgta
    17941 ggtatattta ataaagattc agatttgatg gatatagcag taagaggtat aagcctaaat
    18001 ctaattacat tgcctataat ggggatttct attgtaggac ctgtgtattt tcagtgtata
    18061 agtaaagtta aacattcaat gtttttaact cttttaagac aatttatttt atttatacca
    18121 ttaatcattg tacttccaat tcaatttaat ttagatggtg tttggttggc acagccagtt
    18181 gcagatttta ttgctatgat aatcgtatta ttattcttaa aaagggaatt taaaacaagc
    18241 cgagtgtaag aaatttagaa aataaaatta aattggaaaa taaaaagact tgtctttata
    18301 aaatgattaa aattatttta tagagacaag ttttttattt tatttaaacg gaactatcca
    18361 tactggtttc ttctagtttt tttccaaaag tcattttctc aaatatttct tgaggtagag
    18421 gttttgaaaa aacatatcct tgtatcatat cacaattaat ttttttaaga taatccattt
    18481 gcaagattgt ttcaacacct tctgatatac tattcatatt taattttttt gccaaattaa
    18541 ctatagattc tattacatga ttttcagcaa tattatctgc attaggtgag ctaaaaaaag
    18601 cacggtcaag ttttaatgtg tcaactttta tttcttttaa catattgagt gaagagtatc
    18661 cacttccaaa gtcatctagt gaacattgaa aaccttcttc atggatttgg tctattatat
    18721 taattaattt ttggagattt tcaaatacaa gtgtttcagt taattcaatt tctaagagct
    18781 tagcaggaat gttgtattta ttacgaagca ttctgtattt ttctaaaaaa tctatattat
    18841 ttaaattagc acgtgaaaaa ttaactgaaa caggtattgg agtttctcca ttatctatcc
    18901 attttcttat catactgcat actttttcaa aaacatagtg gtcgagtttt gtaataaaac
    18961 catttttttc aaagaaagga ataaactcgt ctggtggtat aagaccttta tttgggtctt
    19021 gccatcttac taatgcttct gcacctacaa tagtattatc tttgaggctt attttaggct
    19081 gaagatatac tataaattcg ttattattta atgcttcctc cattctattt tccatttctt
    19141 tttctttaat catttttaaa cgctctaaat cactataaaa aacacatgta taaagtggat
    19201 gatttccaat gtttccttta ttattttttc gtgcaacatt tgcacggtct tgaattgaaa
    19261 ctaaaggtag atttgggtca tcaattttat atacacctat agatagagct ataaaatatt
    19321 tacattcttc atttttattg aaactattaa tatcattggt gatattttct atgtacttaa
    19381 gaatttcttt ttgaggaata gttttaataa gtacattaaa ctggtcatta gaaactcgac
    19441 aaagcaattc tcctggttct agataattac taatgacatc atgtatgtac tttaatgttt
    19501 tatctcctgc ttcactacca aaagaatcat taataagttt gaatttctga atatttatag
    19561 ataccaatga atagctttct gatgaagatg attgtataat ttttttagct tcaatatcaa
    19621 aacgtgcttt actgtaacct ttagttacag agtctacata ggcgattttt ataagttgtg
    19681 ccctgttctt ttgatttata aacataatca atataataag caataaaaat agtataacaa
    19741 ttaaaatatt gatgaagatt gcttttttta gaagtaaatc aaactgtgca cttgctgttt
    19801 ttgttggaac aattgaaagg aggtataagt tttcttcttg taatggaaga tagtgcattg
    19861 ccttagaaat accatcattt aatgtataat acatagaacc agttttgttc ttttttaagt
    19921 ttattttcat ttcttcaagt ttatttttat caacctgttt tccttggtgt ttaatcatgt
    19981 caaaaaaatt taccacattt tttggagcat ttttgtttgc tgaacttaca ataaaatttc
    20041 catcttggtc gataatttgg gaaaatcctt caccatgaaa gctttctaca cttaataaat
    20101 ttctcatagt atcaatagta ttccaagcag caacagctcc aacaatttta tcgccattgt
    20161 atagtggtac agaatataaa attccatatg taccagattg tgatttaaca ggagtaattg
    20221 tttctttgcc ttgaagggct tttctataaa agtcttgtcc tttaacatta cctgtatctt
    20281 taagggcact tacaaaattt ccattagaat ctataatacc taaccaatca aattcaaata
    20341 attttaattt ttttgataag tatctattga cttctttttc actatcatta atctcaaaat
    20401 aagtatctcc catagaatat agtgtcctaa atatactttt acatttcaca ttaattgtag
    20461 tggaaatacc ttgtgaaact tctacaagat atcgttctgt ttcattatgt gttactttat
    20521 ttagattcat aataaaagtg acacatgtta atgacacgat taaaaggagt gtcaatagta
    20581 aagtagctat tattttttta ttttttgtgt tttcaaagtt cattcttttg tttcatcctc
    20641 catcctttat ttgtttacaa ataagaattc tctcagacat attataccaa atatcttttc
    20701 atacaaagat attaaatttt atttatccca ctttacattt agctttattt atgtcttctt
    20761 agagattaat caagatacgt ttattgttta aatcttattt tataatattt tgagtaaaag
    20821 aggttaaaaa aagaactgtt ttcaaaataa ttttgataca gttccttttc agtatagaaa
    20881 atttttatag tattagaaag aacttttagc ttatgtatct acataagtat tatatttata
    20941 aaagatattt tgttacaatc taaaatgtag atgaaattct gtaaatgcca aattctctat
    21001 gaccaagact ttcagctttg gtatgtttac cattacagac ttctattatt tcattaaata
    21061 tttcttgacc tatatctgct attttagcac catctgttat aattctacct gcatttatat
    21121 cgatattgtc tggcattttg ttgtatgtat cactattccc agtgatttta ataactggtg
    21181 aaatagggct tcctgttgga gtacctctac cagttgaaaa gattacaatt tgtgcaccac
    21241 ctgctaccat accagtgata gaatctatat cttgacctgg agtatccata aaatataatc
    21301 cttttttatc aggaggtaca atatcagcat attcaagagc acctttgaat ggcttattgc
    21361 ctgccttata catacaccca agtgactttt cttcaattgt tgaaagtcca ccagttttat
    21421 tgcctggagt aggttgacca ctacgtaaat cttctcccat agcaattgct cttttttcta
    21481 catcacttac aaatttaaga aacttatctt tcatttcttc atcttcaaaa cgggtagcca
    21541 acaaatgttc tgcaccaatt acttctgtag tttcacttag tatgctactt ccaccttcat
    21601 ctacaagaag gtttgatgca acgcctattg aagggtttga agctattcca cttgttgggt
    21661 cagaaccacc acattcaagt gctaatgtaa tttcactcat accaaattca actcttactt
    21721 cttttgacac ttcacgagcc attttactta ctatttcagc tcccttagaa actgttttta
    21781 atgttccgcc acattcctgt ataacaacgt aatctacctt ttttcctgtt gtagctattt
    21841 cagatgctaa ttcttttgct tgaataccat cacatccaag accaactact aaaactgcgg
    21901 caacattagg attttttcca aatcctatta atgtctttgc tgtaagctct atatcagaac
    21961 caatttgaca acatccatgt tgatgaggga ttgctattgc tccaggcaca agtgatgcta
    22021 ttcttgtagc tgtttcactt gaacatacag atgttggaat aattaaaaca tgattacgaa
    22081 taccaaactg accattttct cttaaataac ccattaattt cattttaact tcctcctatt
    22141 ttttatctcc acgaccacgt gtactttcaa tgttatgtac atgaacatag tcaccagttt
    22201 ttatgtcaac tgttgcaatt cctataattt caccgtattt gtatacaaca tcaccttttt
    22261 ttacatcttc tgtagccatc ttatgaaaga caggtatatt atcatttact atgtaagact
    22321 ttccatcaat tgaaactttt tcacctttaa ctaaaggtct gacacatgta actagattat
    22381 ctttttcatt tgcatgaata attgcattca ttaattttac ctccttaagt attactttac
    22441 ttcaatattt ttatttgttt taaaaataga ttttttcttt ttttctcttt tatctagata
    22501 ggaaactaac attggacaac atattgctgt aacaataata gcagcagcaa cctgtactgt
    22561 tgaaacagct gctattgtag ctagagtagg gtctatagct gctaaagctt caggagtacc
    22621 agctgcattt ccggctgttg tacctgtagc tgcacctact gctggatgtt ctgcacgtat
    22681 aagtttcatt acaaagtacc cacccatacc tgttattata gtaactccta aaccaagtac
    22741 aactccaggt agtccagcag ttataatttg attaaagttt aatgctgctc ctaaaggaaa
    22801 agcgaagaaa ggaattaata atgttgtacc aggtgcaaga aattctcgta tatcttcatc
    22861 aaggttacca agtataaatc caagtagtat tggtataagt gctgcaaata gtgctactat
    22921 aggtacattt gcaagtccag ttactccaaa agcaagcatt gtaaaaaaag gaccatcatt
    22981 tagagataag atagatattg ctccaacatc tgtggagtct ccatattcac ctgcaagagc
    23041 agcaaataga cctccatttg aattagtcat ggtagcaact atggcaagag gtgtaatacc
    23101 aattatacct ttaggtccaa taaatttact aacaataata cctaatactg caccaattat
    23161 aaattttgtt gctgtaagta ctataccttt agtcaatggt ttaccagctt gtctgatatt
    23221 gatttgagct ccattgcata gacaaaatag agcaagtata ctcattgcac cagttttaaa
    23281 taaggatgtt gtgaatcctc caatttcaag agcttgtgga catactgtat taaatagtgc
    23341 tccaaataac aagggacaga ccataagtcc tcctggtatc tttttcactg attttaagat
    23401 gtgcatctag tatcactccc tttcattttt atataggtat tataaaagca attactatgc
    23461 caatatatag agtttgtatc tttagttatc atgattattt ttaataatcg attgaaatgc
    23521 tgtagattac ttataattaa aagtatgatt gacgagaatt tcacttacaa agattctttt
    23581 tatcatataa ttgtattttt tattgaaaac aaataaacgt cttatgtatt aaaattaaac
    23641 ataaaacgtt ttgatatata tagatatgta ttatttttaa acatcatctt ctatttcttt
    23701 taacttacgc catagagtgg agcgagttat ctttagaatt tcacaggttt ttgtagtatt
    23761 tctattattt tcgttataaa ttttatagat atattttttt tctaaggttt ttaaatctaa
    23821 attactgcat tcaaaatcac tattatcttt atcaactata ttatttgtat attcattgct
    23881 aagcttttct aataaaatcg agtcaaatga ttccacaata acagcccttt ccatcatacc
    23941 ttccaattcc cttatattac cttcatattc tcgttcaaga agtgcctttt taatatttgg
    24001 agtgataatt aaaggcgttt tattgtattt gcttgaatat ttatgtataa aaaattctga
    24061 taattttata atatcttctt ttctttcttt taatgaagga agatataatg ttagtatatt
    24121 tattctaaaa taaaggtctt gtctgaataa accattgcgc atcatatttt ttaagtcttt
    24181 atttgaagca cagataattt ttacatctat aggaataact ttatcaccac caattctcat
    24241 aacttgtttt tcctgcaata ccctcaataa tcgaccttga agttcaagag ggatttcact
    24301 tatttcatct aggaagattg tgccttcatg tgctagttcg aatacacctg ctttaccttt
    24361 ttttctagca cctgtaaatg aaccttcctc ataaccaaaa aactcactct ctattagaga
    24421 tggaggtatg gcagcacaat ttacagcaac aaaaggtgca tttttacgct tactatagtt
    24481 atgtatactt tgacaaaata gttctttacc aactccagat tctccagtaa taatcatagg
    24541 agtatcataa caagcatatt tttttgcaag ttctatacaa tcttttgtct ttttactagc
    24601 atgaattata tcatcaaaag tgtatttagc agatagacct ttgcttaata agctccttct
    24661 tattgtatgt tcaaaagaac ttatctcctt gctatcttga aatgttgaaa cagccccttg
    24721 aatctgacca tcaacaatga ttggaactct atttgtaata atttttgtac tccctatatt
    24781 ttgtatttca ccaatttgag gttccatagt ctcaactact tgtggaagaa ctgtgtttgg
    24841 gataacatct acaacattat gataaagtgc attttctttt tttataccaa atattttttc
    24901 agcactctta ttaaataatg ttattttaaa atttttatca actgttatta ttccatcatg
    24961 tacaaaatct gtcatagctt ctatctgtaa tcttcttttt tgttcatcct tggttgcttc
    25021 gagtatttgt tctgcttgtt gaatggctct aagtatgttt tcttctctag attcgattaa
    25081 tattccctta caattaagtc tcttaacaat atgtgcaaca gtggtatctc ctatatatgt
    25141 gtttatacct ttctttctgt acttttctat agcatcataa atttcttctt ctttttcaat
    25201 ttcaatcata gttattttct tattcaatat ttcttctata atatcgaaac cgtagatgac
    25261 attattaaat ccaattattc caaatggttc attggaattt tttacctcct tgtagctttt
    25321 tataatatca taagcatcta ccttaatttc aactataggt atattgtaag tagcttttaa
    25381 catattgtaa gttccacctc ggcttatgat aattctagta ccatgattaa tacttttttc
    25441 agcaagaggt ataccttctt ttagattgcc aaatacaaca tcaatatttg tatatttgtt
    25501 ttttttaact atgtgaattg atttttcata ggttccttta gtgggagcaa taacgagtat
    25561 ttccttcata ataattcctc catatttcat caaataaact taaatattat cttatgacta
    25621 aattttatca gttatatttt aacattttac tttatattta attataaata aagtattttt
    25681 aaaaggaaca ttataaaaga ctgtatcaaa gttaactttt agaattataa agtttcatat
    25741 ttttacagtc ttttaatata taatatctat ttatcaaatc tatctacttt aaataatttc
    25801 atatttttat tttgtttgcc aagatataat tcacttaaca ttatacctcc taatattgca
    25861 aagagtatac catttgctcc ataacctaag caataccata gattgttatt tttattatct
    25921 ggaccaataa aacctaaatt atctaaagtt gaagcaaaac aaccacaata tttatattca
    25981 acttcaatgt cttttatatc cttaaacata cttttcaatc tttgttctaa aatatcatac
    26041 ttttcattgg ctaaagtttc attttctata tcatcaaaac ttacatcttc accacctata
    26101 attaatcgat tgtctttagt agttcttaga taattatatg ggtcacagtt atctctaatt
    26161 aaaatgttgt ttttccaaga agctatgttt ttaagtggtt tcgttgctat attaaaagta
    26221 tttgacttag ttgcaaaatt tctttttgta aataaacttg tattgtaccc tgtagctaca
    26281 attaattttt taccatgaac tttgtatcca tacgatactt cagcagtaac cttatcattt
    26341 gataaatcta ctttttttac ttctgtattt tcatatactt gaagaccatt ttttaaactt
    26401 acatcaatca aatggtgact atatttatat gggtctaatt ctcgtcctcc attctttgct
    26461 atcaatcctg attttaaatc aaagctaaaa gggtttgtgg attcatcaat atattcaaca
    26521 tcaaatccat tttcttttct taagttatat tcttctttga tagccttaac ttcaagtttg
    26581 ttggcagtat aaagtagagt atctcttttg gcataatcac acttatttcc atatagctct
    26641 ataaatgtat ctaattcttc aagtgctgaa gtacaaaggt tgtatgcttt tagagcatct
    26701 tttagagtca taacctctgt taaatctatc aaattgtcat ctaattcata ctgtaataga
    26761 gaagttgtca cgctagtgct tccatgtgca atccttcctt tttcaagaat gacagacttt
    26821 atattgtttt tagctagata gtaagcacaa atacatccag ttacgccacc accaacaatt
    26881 ataacatcag tatcaatatc atcagtaagg tatggaaatt gtcttggaac tttattaata
    26941 ttagtaaaaa taggatttcc ttttacatat tcttgattca tatacttcac tcctaaaatt
    27001 tattatgtat gagtgtatta ttttctatag caaataatat tatacatttt aattatctag
    27061 ttatatgggt atgtatagat aaaatgtgat aacattttag tataaaacat atgtataata
    27121 tattcataat taccttattt taagtaatat agtatataat gtttgttaac aagtaaaaag
    27181 attgaataaa agttaattaa aaatttaaag taggaggtaa atttatgctt tcaaaaaata
    27241 tagcttctaa ggtattagaa aaatgtctaa ttactggagg agattttgct gagatttttg
    27301 aagaagacac tataaataat tctattagtt taatagataa taaagtagaa aatgcaatag
    27361 gtggaagaaa ttatggtata ggaataagaa tatttaaagg attgaaaagt atatatgctt
    27421 atacaagtga caattcatta aatagtctgt tggatgtagc atacaaagct gctgttgctt
    27481 taggtaaatt ggaagatgga aaaagtgtaa tattaaatga cagtattaaa ataaataaca
    27541 tacataatat aaaaatttat ccaaattcaa taggaaataa agataaagta agtgttatga
    27601 aggttgcata taagagtgca aaagaattta gtaatgatat atctcaagta agtgttagtt
    27661 atttggataa agaccaaaaa atattaattg caaacactga aggtatatat gttgaggata
    27721 gaagaataag aactagacta gggataagtt caattgcatc taagggtaat gaaaatcaaa
    27781 ctggatttga aggccctgga ggttgtaaag ggtttgaaat atttgaagaa atagacccag
    27841 aatattatgc aaaagaatca gccagagtag cgcatactat gttgcatgct aaaaattgtc
    27901 ctgcaggtaa tatgatggta gcaatagata atggatttgg tggagtaata tttcatgaag
    27961 cttgtggaca ttctttggaa gctacatcag ttgcaaaggg gaactctgta tttgcagata
    28021 aattaggtca acagattgct tccacaaaga ttacagctat agatgatggt acaataccaa
    28081 actactgggg ttctatgaat atagatgatg agggaaatcc aactcaaaag aatatactga
    28141 ttgaaaatgg aattttgaag tcttatatga tagataaact taatggtaga cgaatgggaa
    28201 tgaatcctac aggaagttct cgtagacaaa actacaaatt tgcaccaact tcaaggatga
    28261 ctaacactta tattgctgct ggagaagata gaccagaaga tattataaag tcaataccag
    28321 atggacttta tgctaaaaaa atgggtggtg gttctgtaaa tcctgtaaca ggagagttta
    28381 attttgcagt ttcagaaggt tacttaataa aaaatggaga aatacaagag ccagttagag
    28441 gtgctagttt aattggaaaa ggtagcgata tacttatgaa tatagatatg gttgggaata
    28501 acgtaaaaca agctcagggg atgtgtggct catcatcagg aagtatacca actaatgtag
    28561 gccaacctat gataagggta aaagaaatta ctgtaggtgg aagataggag ggataattgt
    28621 ggaacttaaa aactttaaag atatattatt taaaaaggct ctaagtgaag gttttgaaga
    28681 gtgtgaaata tattattata caggagaaaa tttaagtata aatatatatg aaggagaagt
    28741 tgagaaatac aatcttgata aatcttttgg tttatctttt agaggtaagg taaatggaaa
    28801 gataggttat tcttatacag aaattttaga tgataaagct gttgatatgt tgataaaaaa
    28861 tgtaaaagat ggagtaaaca ctattgaaaa tgaagatgta caatttatat atgaaggaga
    28921 taagcactat aatgatgtaa agacttattc taaagagcta gaaaatttag aggcagataa
    28981 gttgattgat ttagctttag aaatggaaag agagactaaa gcttattcag ataaggtggt
    29041 aaatttatct aaatgtacta tatcttattc tgtatcaagt aatggtatat ctaatactaa
    29101 aggtcttaat ttaagcaata aaactaatat gcttataggg tttgtagttc ctatcataga
    29161 agacaatgga caaaaatatg atggaatagg atataatata gcaaattcta ttgaagaaat
    29221 aaaaccttgt gagatagcaa aacttggtgt tgataatgct ttatctaaag taggtggtaa
    29281 gagtattcca tcaggtaaat ataaaactat attgcttaat gaagctatgg tatctttgct
    29341 tagcacattt tctgggatat ttaatgcaga ttcagctcaa aaaggattat ctttattgaa
    29401 aaatagagaa ggagacatga tagcatctcc tatagttaca atagttgatg acccactttt
    29461 agaaaatggt atggcatcta ctccttttga tgatgaagga gtagctacat ttaaaaaaga
    29521 agttgtttta aatggaaagc ttataacttt acttcataac ttaaaaacgg ctaataaggc
    29581 aggtgtaaag actacaggaa atggaataaa atcttcatat tcatcaccta taagcatata
    29641 tccaactaat ttttatatag agaaaggaga taaatcttta gatgaaatta taaaggatat
    29701 agatgaaggg ttgatggtta ctagctttgc agggcttcat tcaggtgcaa attcagtaac
    29761 aggagacttt tctttagctg ccaaaggctt ttatatcaaa gaaggaaaaa aagtatttcc
    29821 agtggagcaa attacagtag caggaaacta ttttgacctg ttgaaagata ttgaagttat
    29881 aggggaagat ttagaatttc caatgagtag tataggttca ccttcagttg ttattaaaga
    29941 gctatcagta gctggaaaag atgaataatt aaaaagtaat tttgtggata aaaggtcatg
    30001 ttaattttaa ttaatatggc tttttattat ttaagtagat tttattatct aagttatatg
    30061 aaaaagttgt gatgcataca attaatatca tacattaaat ttaaaggagg agatattatg
    30121 agtggtgcac attatgacat gcacaagcga aatggatatt caagtgatga gccatatcca
    30181 gagataaaag ttttaggtcc aaataagtat tatgcagaat tattgatgga tgactatgct
    30241 ggagtatcaa gtgaatttac gagtgtaaat caatatctat accataattt tgatttagat
    30301 gaaacgcata gagagttaag tgaaatgtgg ataaatatat ctataacaga aatgttacat
    30361 atggagatat tagcaaagac tataagatta ttgggtggca atcctgtata tagaggttct
    30421 acaagttctt gtggtgcata ttggaatggt ggttttgtat gttatggaaa ttctatttgt
    30481 aacagattaa aactagattt gcatttggag catgtggcta ttaatcatta ttataaagat
    30541 ataagtctta ttgaagaccc ttatataaaa gctatattaa atagaataat cctagatgaa
    30601 aaacttcatg taagtttatt tgaaaaggca atagagaagt attgtaaata aatttgaaaa
    30661 tttagttatt catcatttta ttcatatata taaaataacc atcatacatt ttcttgaagg
    30721 gagttgctgt tgatatgtat tatgtatatg tgaatgatat aaacatagca gtatatgatt
    30781 tgaatccgag tgctaaaaaa actgtacttt ttatacatgg ttggccctta ggacataaga
    30841 tatttgaata tcaaacaaat atattgccaa aactcggata tagaacagtt tcaattgatt
    30901 taagaggttt tggtaaatct gatgcaacat caggaggata tacttatagc caattagcag
    30961 acgatattta taaggtggta catgctattg gattaaaaga ctttaccctt gttggttttt
    31021 caatgggagg agcaatcgta cttaggtata tgagtttatt taatggatat ggagtgtcaa
    31081 aattggtatt agctgcagct gctgcaccat catttgtaca aagacctcca gaatttccat
    31141 atggtatgac acgtgaagat gtaaataaat tgatagcaca agcatgtact gatagaccag
    31201 aaatggtaac cgattttggc gaaaaagtat ttgcatcaaa tccaccagaa agctttagaa
    31261 gatggtttaa tgatattggt tttagtgcat caggtattgg cactattgga actgctgtat
    31321 ctttaagaga tgaagaatta tttaatgatt taaaatgtgt aagagtgcct actggtatat
    31381 ttcatgggaa attagatgaa atatgtccgt atgaatttgc agtttttatg aatgaaaaaa
    31441 tagaagattc aattttatat acttttgagt atagtggtca tgctatattc tatgatgagc
    31501 ttaaactatt taatcaggaa tttttacagt ttttagagga atagatatat tttattatca
    31561 cttagaaaaa gagtaatatt atgtgaaaag atttaagcta aattttatga tgttttttac
    31621 actaaacaaa tagggtttta gaacttgtaa ctaagttcta agaccctatt attatatatt
    31681 cctaaaattt gttataattt attccttgtc attcttaaaa tgtattaata tattaacgaa
    31741 tttatattaa atttttgttt atattccaat aatgtcaaac taaccgtttt agaaattaaa
    31801 ttatatgtga aaaaagatag cttacattat tttattatgg aaaataatag taaatggtac
    31861 aaaatttgat ttttaagatt taaaactttt ttaaaaagtg ctacaataga ccaaattatt
    31921 actttctata ctcgttaaac aggaggtggg actatgaaaa aacataatat tttatttgta
    31981 tcaactgatg acaaaataaa tatagatata tcaaaacaat tggaaaatat atttggagaa
    32041 ttttgtaata tagataactt ggtatatgta aataggataa atattgaact gtcaagctat
    32101 gagcttgtag tatgttcaga caatgatata aaggaatata tacataataa tattgataaa
    32161 aatataccta ttgtaatagt acatcgtact ataaatattg aaaatataaa tcagataata
    32221 agtatagaaa atgattctga tgttatggtt atagatgctt ataaagagtc agcagatgaa
    32281 actgccaaga taattagaaa actaggatta attcatatta atctaatacc atattatcca
    32341 ggttgtgata agtctaaatg tgagattgga attattacgg gaagcagaaa cagcatacca
    32401 caaaacataa agcaaataat tgacataggt gataaggtaa ttgatataaa tacagttata
    32461 gaaatcttta caaaactaaa tatatctata gataaactac atattattaa ggaaaaatac
    32521 aatgaagata cagtaagtgg atataggtat tatacaacta tgaataaaac catgaagagt
    32581 tttttggaga ttatagatga aggtattgct tctgttgata aattaggaaa atttatatat
    32641 tgcaacaaag tattttctaa tctagttgga atagaccaaa atgaaattat atctaataac
    32701 tttatggact tatttagtga taaggttgtc aaaaaaatat tttttcaaga agatgaagtt
    32761 aatgatgaag tagtaaattt aaataacaaa aaactgataa ttaataaagt aaatgtttat
    32821 gaaaataatg aacgaataaa gagtattata agcattaaaa atataagtgc aattcaagtg
    32881 cttgaggaca agattcaaaa taaacttcag tcaaaaggtt ttgtatcaaa gtatacattt
    32941 gagagtgttg taggtgagag taaaataata aaagaaaaaa ttaatattgc aagaaaaata
    33001 gcaactacag acttttctgt gttgatactt ggagaaaatg gaacaggaaa agaaatattt
    33061 gcccaggcaa tacataatga atctctaaga aaaaacaagc catttgttgc agttaatcta
    33121 tcttctttgt cagatacact tattgaaagt gaactttttg gctatgatga aggaagtttt
    33181 acaggagcaa taaaaggtgg aaaaatgggt atatttgaaa gagcacacac aggaacaatt
    33241 tttttagatg agataggtga tatatcttta gatgtacagc agagattgct aagagtatta
    33301 caggaaaaag aagttatgag aatgggcgga agtaaaatta taccaataga tgttagaatt
    33361 atagctgcaa caaataaaga tttaaagaaa aaaattaaag aaggttcctt tagagaagat
    33421 ttgtattata ggattaatgt attacatata gagataccta gacttagaga acgtaaggaa
    33481 gatatacctc ttataagtaa gtatttttta gatgaaataa atagcaacaa atgttttaca
    33541 gaggaatcaa tgcaagcttt aacattgtat gaatggccag gaaatgtaag agagcttaaa
    33601 aatttaattt attatattga tactatagtt gaggaagata gagtagatta cgagcatttg
    33661 ccagaacaat ttagatttga aaaaaaaaat actttagtta atgaaaattt cgattcaatt
    33721 atcttagatt tcaaacaatc taatttcttt gaggaaagta tatgtatatt gaccagtata
    33781 gaaacttgga ataataaaaa tatattatta ggaagaaata aactgcaaga gattttaaaa
    33841 gaaaaagata tagttttaag tgtagaccaa attagaaaaa gaatagataa gttaaagagc
    33901 catggtttac ttttatctgg agtaaaaaaa caaggtagtt ttataactga tgaaggaaag
    33961 aactttatat catatgtaaa aattaaggga gtgatatagg ggactaaaaa ggcaaaacac
    34021 cctaaaaaaa tccctataat ggatagaaat aaataaaatt atctttaaga ttaagataat
    34081 tcaaaaatat tgaataaata atctaagaag attattttta ataaaacagt agtaaatatc
    34141 aaccttacca ctgtttttat tttttgcaag atttattata caattaaaat ttatattttg
    34201 gcacattaat tgctacatag gaaaaatata aaaaattgta gaacttggag ggtgttgtta
    34261 tgaagtcagc ttacaaagga tatgaagcct atagttattt agaaaaagga aaagatattt
    34321 catctattga gatgtgtaaa ggagataaaa gagttaaaga gtaccttatt gaattagatg
    34381 atattcaaga agcgaaggtt aaaagtatag tggatgaaaa aatatttata tcattacatg
    34441 aacatccagt attatttcca gaacacctag aaagagatat atttgaatat aatagggaag
    34501 gtaaacaaag atgcgcttat gaagcattat caagaggata ttatgatgct atatttgata
    34561 atttaatgga tggtgtatgt acgataacat caaattcagg gtggaaatgg gatgatatat
    34621 taatagattt aggcatgaga ctatgtgatt tagctcatca agattttgtc ataaagtgtg
    34681 aaaaagtaga agatatatac agagctaaaa gtgaaggtaa agtagcattg ataccaacat
    34741 tagaaggtgc agcaccaata gaaaatgaac ttgacagaat agatatactg tatggatttg
    34801 gtataaggtt aatgggtata acatacagcg aatccaatgc attaggttca gggctaaaag
    34861 aagagaaaga tggaggtctt acaaatttcg ggaaaaaagc tgttgaaaga atgaataaaa
    34921 ttggaatggc aatagattgc tctcacgttg gagttcaaac tacacttgat gtaatagagt
    34981 atagcaagga cccaataata ttaagccatg ttggggcaag aagtttatgg aacacaaaga
    35041 gactaactcc agatgaagta ttaatagcat gtgctaaaaa aggaggggtt ataggtgtag
    35101 aagcagcacc tcatactaca ataacagaga agaataaaga acattcaata gagtcattta
    35161 tggaacattt tgagtatatt aaaaatttag taggaataga ccatgtagct tttggaccag
    35221 atacagtata tggcgaccat gtaaaattac actcagtact agctgctaaa tttgatataa
    35281 aaaaaataac aggtacaaaa gaacatccca aagtagaata tgtaaaaggt cttgagaacc
    35341 caacagaaac ttcaaaaaac attgtcagat atttagtaaa gcatggatat agtgatgagg
    35401 aaatagagaa ggtactaggt ggaaatatac ttagagtact aaaacaggtt tggaaataga
    35461 tatattagaa atattaataa aatttgagga tttataggga ggttttataa atggattatt
    35521 taaaattagc taatcatcca cttttgtgga tagcgagtac agtagcagta tcaatagtag
    35581 tggtacaatc ccttatattt gctataaaat cttgcaaagt tggtaaaaca atgggaataa
    35641 cagataaaca aataaagtca gctgttaaga gtagtgctat ttcagctatt gggccttcaa
    35701 tgactatatt tgctggaatg gtatcgctta tagtaactat gggtgggcct atagcatgga
    35761 tgagattatc atttataggt tctgtaattt ttgaatcaat gtcagccgga tttggaacag
    35821 atgcattagg tataacttta ggttctcctg aaatgacaaa acttgcattt acaaatgcag
    35881 tttggactat gatattaggt tctttaggat ggataatatt cacattacta tttggacata
    35941 aactagataa gataaccaat gttatatcta gtggcaagaa atcatttata ccaataatat
    36001 ctctaggagc aatgttgggt tcatttgcat atctaaatgc tgatagggtg ttgcgttttg
    36061 ataatggaac aattgcatgt ataagtggaa tggctataat ggtaatattg tgtatgcttg
    36121 aaaagaagaa aaatataaaa tggttaagag agtgggggct tacaatatca atgtttagcg
    36181 gaatgataat agcttcagta atataagggg ggagttagtt ttatgagtaa agtaaaatct
    36241 atatacaatg aagaatattt accttttatg ataaggtatg gaagattaac actatctttg
    36301 ggtattatag cagcattagt tcctggaata attttgagct ttggatttgg tattatgcct
    36361 ccaatatcag cattattagc aagtacaatg gcaattgtca gtatgagtgc tccaaattac
    36421 ataatagaac cagtttctta ttcaccaatc ttaggtatac caggaactta tatgagtttt
    36481 ctttctggta atatatctaa tatgaggcta ccatgttcaa tagcagctca aaaggcagca
    36541 gaagttgaat caggtacaga agaaggctcg ataatatcta caataggaat agctgtatct
    36601 attttagtga atataagtat actaactata ggggtaattt taggaggaag cgtactatct
    36661 aaaattccag ctgaagtagt agaaaaatta aacttaattt taccagctct atttgggtca
    36721 gtttttggac aagtattttt acaagataaa aaattaggtc tagtagcaat tgtaatatct
    36781 gtattgacta ttatattatc aaagcaaggt ataataccac agtctctagt agttcttata
    36841 tgtgtatttg gtactatatt aattgcaaga gctatgtata aagataagct tagtgactaa
    36901 ttacaatgaa ataatagtga attttcaaat atgaaattta ataatttgat tttcaaatat
    36961 aaaaattaca ttaagattat ttaaaaattg gtttttaagg agggaatatt ttgatatatg
    37021 atttaatctt aaaaaatgga tttataatag atggtacagg taatcctggt ttttatggag
    37081 atatagcaat taaagacaac ttaatagcaa aaatagattc taagataaat agcaatataa
    37141 ataaagagat tgattgttgt ggaaaagtta tgacacctgg atttattgac cctcatgttc
    37201 atgaagaaat agtagcaata ctagatggta aatttgaaaa gtttctaaaa caaggagtta
    37261 caacaactat aaatggtaat tgtggacatt ctataacacc atattcttct gaaaatgtgt
    37321 atgaatatat gtataaaaat ggtctattac ttgaagaaga aaaaaagtat cttatagata
    37381 aaaataaatg ttggaataat tttacagaat attgtgacct tatatctaaa agtggtattt
    37441 ctgtcaatat gggattcttg ttggggcatg ggactataag atggagtgtt atgggaggtt
    37501 ctaaggatag aactcctaca gaaaaggaaa aaaatgaaat tacagatata ataaatgatg
    37561 gaatgaaaag tggtgctttt ggaatatcta ctggattggc atatatacca agtaagtatg
    37621 ctgatattga tgaacttgtt gatattgcaa ggcagattaa agagtatgat ggtatatata
    37681 catctcatat tagagattac ataggaagat ataatgcagt aaaagaagcc atagaggttg
    37741 ggcaaaaaag tggtgctagg gttcaagtat ctcacttaag tccagttgaa atagaagcat
    37801 ttgatgagat attaaaggct aggtataatg gtgttgaaat aatggttgac acagtaccaa
    37861 gaagttctgg gcattgtatg aagaaaaaaa gagtaattca atttattatg gcaatatcat
    37921 ctagtctatt tgaattaggt atagatgggg taatggatgc tcttagaaat gaagagggaa
    37981 gaacacttat attaaaagaa gcatttatac ttggagatag ggggagtata atacttctaa
    38041 acactaaaga tattaatatg gaaaagaaat caatacgtga aatagctaca caaaaaggaa
    38101 tagatgagga caagttatta ctcgatttac ttttagatgg agacgaagaa cttatctttt
    38161 gtttaggagg aatgtataga gctgatttcc cagataaact acatgatgat aaaataattg
    38221 ataatccatt tgttatggtt ggttcagatt gtttgtttag tgtgggtggg gatatgtcat
    38281 ggtttgaact acaaagaaga ggagcttttc caattttctt caatatgtac agaaagtctg
    38341 gtgttagatt ggaagagatt gtaagaagag ttacttcatt accagctagg caatttaaaa
    38401 taaaaaatag aggtattctt aaagaagggt tgatagctga tatagcagtt attgacatgg
    38461 ataactatag ttattctagg tcagaagata ttgatttttc aaagccacaa ttactagcca
    38521 atggtgttga atatgtaatt gtaaatggaa aaattgcatt ggaaaatgga gagattactg
    38581 agaataaatg tggtgaagta ttaaaaagat aaggttttaa aaataatagg atttagaaag
    38641 taaataaata tctaagtaag taaagacgaa gtttgagatt tatttaaata aaaatcatgc
    38701 ttcgttttta tttatagtat taatatttat tttaataatc agataatatt tgtactttat
    38761 agtgaaaaaa ttaatatttt taatcattaa attatgtaaa aaattggata aatagataac
    38821 taaatcattt gagaaaagat ttttagaaat aaaaaatgat aactaaataa cccctttaat
    38881 atttatcaaa tagccagtca taaatattaa aaggggttat ttagtttata aaaattattt
    38941 gtaataaagt aggatatatt aataaatatg atagaatata tattataaat taaatttata
    39001 aatataaggg gggaactttg agttcttata gatttcttac attataagaa ccaatacttt
    39061 atgcgggagt tattaaaagt aacacttata gtaatgcttg ttttgataaa tgcttttttt
    39121 gtagcttgtg aatttgctat ggtaaaactt cgtagttcta ggattgatac catgataact
    39181 gaaggtaata ataatgcaaa aagatgtaaa ataataaaag acaacttaaa ttcctatctt
    39241 tctgcatgtc aacttggtat aacactttgt tcacttgcat taggttggat gggagaatct
    39301 acagttaaag agttaatttt accaatagta agtgtattta atcttagtga aagtattata
    39361 tatactatat caatttccat ttctttttta attataacaa tgatggaggt tgtaatagga
    39421 gagttggttc ctaaagcttt agcactttat aatacagaaa aaataatgtt gagtacatct
    39481 tttttattag taggatttta taagttaaca tatccaataa tatatttttt caacttaagt
    39541 actgatttat ttcttaaacc atttggatat tcacaagctg atgaggttga tgaaccccat
    39601 acaggtgacg aaataagact attagtagaa gaaagttata aaagtggact tatagatgaa
    39661 tcagagcaaa gattagttga taatattttt gaatttgaag aaaagaaaat tagagagata
    39721 atggttccta gaactgatat ggtatgtata tatgaaagtg attctgaaga aaagatatta
    39781 gctattttaa aagaggaagg tgttacaaga tatccagtat gcagaaaaaa taaagatgat
    39841 atattaggtt ttgtacatat aagagattta tacaatcaaa agataaatga aaataaaatt
    39901 gaactagaag aaatattgag agatattata tatatatctg aaaatttaac tatagataaa
    39961 gctcttgaga gaataagaaa ggaaaaactt caacttgcta tagttgtgga tgaatatggt
    40021 ggtacatctg gtgtggtaac aatagaagat atactagaag agatagttgg agaaattcaa
    40081 gatgaatttg acaaagaaga gactcatatt aagaaaatag gagcaggttc ttacttagtt
    40141 gatggaactg aatctatcaa ctatattaat aagtattttg gacttgatat agagcatgat
    40201 ggatttgaca gtataggtgg gtggatatcc tatatgcttg gttcaaatat taaagtaaac
    40261 caaagtgtaa cttttgaaaa ttacaatttt acaatattag aattggataa attgagggtt
    40321 gtaaaattaa tcataaaaag tgtaatataa ttatatcaaa aaatattgga ggaattgtat
    40381 gcaaaaagta gaaggaatta tttttgacat ggatggagtt ttgtttgact cagagagaat
    40441 ttctcttgaa ttttggatgg agacgtttga aaagtatgga tatactatga ctaaggaaat
    40501 ttatacatca gttatgggaa gaaatcgtaa aggaataata gaaggtctta cagatatata
    40561 cgatagttct gtaccaataa tagatttata tgacgaaaaa actaaaaaca tgattgaatt
    40621 tatggagagg aagggagctc ctatcaaatt aggagtcaat gaactaatct cttttttaaa
    40681 agaaaatgga tacaaaatgg ctgttgcaac atcaacaaag agagaaagag cagtaaagag
    40741 attagctaag gcaaatttaa aagattactt tgatgctata gtttgtggag atgatgttgt
    40801 aaactctaaa ccaaaccctg agatattctt aaaagcagct aaaaagataa atgttaatcc
    40861 caaaaattgt attgtcatag aggattcgcc tatgggagtt gaagctgcat acaatggtgg
    40921 tataagatgt ataaatgtac cagatttaaa agagccagat gagcaaatta aatcacaatc
    40981 acataagata cttgaaaatt tactggaagt tagagaatac ctaaaaagtt tgaactctaa
    41041 agaatgtcac aatctataat ataaagtatt aattaaataa attaaatata atttctttga
    41101 tttagaataa atccatgaat atgagaaaaa ataagtataa acagctagga ggaatattca
    41161 tggataatgt aggttctcta ttatattttt tatttaattt tttcttgtat ggttttatag
    41221 gttggataat tgaaaactta tatagttatt ataaacaagg gcattttcaa gaagatggat
    41281 ttttaaatgg tccatttaaa ccaatgtatg caattgcaat gtcgattata atagaggtct
    41341 caagatttat tgaccccaat aatatatttg cattgctttt attatgtcta gtagtgccga
    41401 cagtagtaga atatattaca ggaatactta tgaaagtata ttttaataag gtctattggg
    41461 attattcaga ttataagtac aatagtagag gaatagtatg tctaaagttt tctgtatatt
    41521 ggactatatt gacatttatt ggagttagat attttcaagt gtatatagtt gatagtttat
    41581 atagtcttat aataccttat tggccaattg ttgtaactat acttttagta acattgtttg
    41641 tggatgaaat tcttactata aaatttttga ggggaaataa agatatagta gaaagataac
    41701 tttaaaaata tgagttgaat ttagattgta gttaaaagaa agctatgtta aaatggtgaa
    41761 atcatttaaa attagctttc ttttttattt gataaaatta ttttataata tatctataag
    41821 ttgtaaagtt ttttattaaa attaaaataa agtgtagaaa atggggagtg atttatgatg
    41881 ggtaaaataa gatttggtat tgttgggaca agcaatatag caaatatatt tctaagagct
    41941 gcatctaggg ttaaagactt tgaattggtt gctgtatatt ctagaaattt agaaaaagct
    42001 agagaatttg gaactctata tggagcaagt attttttttg atgatttaga acaaatggct
    42061 aaatcgaagg atatagatgc tgtatacata gcttctccaa atgcattaca ttcaaagcaa
    42121 accattactt gtttaaaaca caaaaaacat gtgctttgtg aaaagtcgtt agcatctaat
    42181 ttaaaagaag tcaaatcaat gataaaaaca gccaaagata ataatgtatt gcttatggaa
    42241 gctatgagaa ttacatgtgt tccaaacttt aaagcagtta aagaaaactt atataaaata
    42301 gggaaaataa gaagattttt tggaagctat tgtcagtatt cttcaagata tgataaatat
    42361 aaaaatggaa ttatagaaaa tgcctttaaa aaagagttat caaatggagc tttaatggat
    42421 ataggagtgt attgtataca tcctatggtg aatttatttg gcgctccaaa aactgtaaag
    42481 gcagtttctc atatacttca aactggagtg gatggagaag gttcagctgt atttcaatat
    42541 gatgatatgg atgcgataat tcagtattct aaagtagctg attcttatat accatctgaa
    42601 atacaaggag aagagggaag tataataatt gaaaagctta atctatttga aaaagctatt
    42661 attaaataca gagatggaag agaagaagat atatctattc caaaagaaaa agcagaggaa
    42721 aagcctagag aaatagaagg aatgtattat gaacttgtag agtttataag tcttataaat
    42781 aacaataaaa tagaatcaaa tataaattct catgaaaatt caataatagt aatggaaatt
    42841 atggatgaga taagaagaca aagtagtatt gtttat
//
LOCUS       DAEMIK010000013        41822 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEMIK010000013 DAEMIK010000000
VERSION     DAEMIK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.013"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            131..319
                     /locus_tag="KPK87_000606"
     CDS             131..319
                     /locus_tag="KPK87_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG2536798.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(479..631)
                     /locus_tag="KPK87_000607"
     CDS             complement(479..631)
                     /locus_tag="KPK87_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG2536799.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(729..1205)
                     /locus_tag="KPK87_000608"
     CDS             complement(729..1205)
                     /locus_tag="KPK87_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2536800.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1424..2185
                     /locus_tag="KPK87_000609"
     CDS             1424..2185
                     /locus_tag="KPK87_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2536801.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2445..4373)
                     /locus_tag="KPK87_000610"
     CDS             complement(2445..4373)
                     /locus_tag="KPK87_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2536802.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4607..5059
                     /locus_tag="KPK87_000611"
     CDS             4607..5059
                     /locus_tag="KPK87_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2536803.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5062..5370
                     /locus_tag="KPK87_000612"
     CDS             5062..5370
                     /locus_tag="KPK87_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2536804.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTIAKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5484..6590
                     /locus_tag="KPK87_000613"
     CDS             5484..6590
                     /locus_tag="KPK87_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2536805.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMMGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6665..9343
                     /gene="mngB"
                     /locus_tag="KPK87_000614"
     CDS             6665..9343
                     /gene="mngB"
                     /locus_tag="KPK87_000614"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG2536806.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRSFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDIIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9660..11993)
                     /locus_tag="KPK87_000615"
     CDS             complement(9660..11993)
                     /locus_tag="KPK87_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2536807.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11968..12684)
                     /locus_tag="KPK87_000616"
     CDS             complement(11968..12684)
                     /locus_tag="KPK87_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536808.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(13099..13755)
                     /locus_tag="KPK87_000617"
     CDS             complement(13099..13755)
                     /locus_tag="KPK87_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2536809.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(13810..14439)
                     /locus_tag="KPK87_000618"
     CDS             complement(13810..14439)
                     /locus_tag="KPK87_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2536810.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(14570..15211)
                     /locus_tag="KPK87_000619"
     CDS             complement(14570..15211)
                     /locus_tag="KPK87_000619"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG2536811.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(15220..15891)
                     /locus_tag="KPK87_000620"
     CDS             complement(15220..15891)
                     /locus_tag="KPK87_000620"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG2536812.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(15896..16780)
                     /gene="rsgA"
                     /locus_tag="KPK87_000621"
     CDS             complement(15896..16780)
                     /gene="rsgA"
                     /locus_tag="KPK87_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG2536813.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANIDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(16878..17615)
                     /locus_tag="KPK87_000622"
     CDS             complement(16878..17615)
                     /locus_tag="KPK87_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2536814.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(17723..19738)
                     /gene="pknB"
                     /locus_tag="KPK87_000623"
     CDS             complement(17723..19738)
                     /gene="pknB"
                     /locus_tag="KPK87_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG2536815.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(19755..20507)
                     /locus_tag="KPK87_000624"
     CDS             complement(19755..20507)
                     /locus_tag="KPK87_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG2536816.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(20527..21558)
                     /gene="rlmN"
                     /locus_tag="KPK87_000625"
     CDS             complement(20527..21558)
                     /gene="rlmN"
                     /locus_tag="KPK87_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG2536817.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(21566..22891)
                     /gene="rsmB"
                     /locus_tag="KPK87_000626"
     CDS             complement(21566..22891)
                     /gene="rsmB"
                     /locus_tag="KPK87_000626"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG2536818.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQNKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(22953..23654)
                     /locus_tag="KPK87_000627"
     CDS             complement(22953..23654)
                     /locus_tag="KPK87_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG2536819.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(23723..24475)
                     /locus_tag="KPK87_000628"
     CDS             complement(23723..24475)
                     /locus_tag="KPK87_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG2536820.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(24485..25414)
                     /locus_tag="KPK87_000629"
     CDS             complement(24485..25414)
                     /locus_tag="KPK87_000629"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG2536821.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(25429..25869)
                     /gene="def"
                     /locus_tag="KPK87_000630"
     CDS             complement(25429..25869)
                     /gene="def"
                     /locus_tag="KPK87_000630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG2536822.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(25887..28412)
                     /gene="priA"
                     /locus_tag="KPK87_000631"
     CDS             complement(25887..28412)
                     /gene="priA"
                     /locus_tag="KPK87_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG2536823.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(28651..29850)
                     /gene="coaBC"
                     /locus_tag="KPK87_000632"
     CDS             complement(28651..29850)
                     /gene="coaBC"
                     /locus_tag="KPK87_000632"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG2536824.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(29855..30121)
                     /locus_tag="KPK87_000633"
     CDS             complement(29855..30121)
                     /locus_tag="KPK87_000633"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG2536825.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(30124..30741)
                     /gene="gmk"
                     /locus_tag="KPK87_000634"
     CDS             complement(30124..30741)
                     /gene="gmk"
                     /locus_tag="KPK87_000634"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG2536826.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(30771..31652)
                     /locus_tag="KPK87_000635"
     CDS             complement(30771..31652)
                     /locus_tag="KPK87_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG2536827.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLSKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(31656..32486)
                     /locus_tag="KPK87_000636"
     CDS             complement(31656..32486)
                     /locus_tag="KPK87_000636"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG2536828.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDYVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(32773..33807)
                     /gene="ltaE"
                     /locus_tag="KPK87_000637"
     CDS             complement(32773..33807)
                     /gene="ltaE"
                     /locus_tag="KPK87_000637"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG2536829.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            34103..35878
                     /locus_tag="KPK87_000638"
     CDS             34103..35878
                     /locus_tag="KPK87_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG2536830.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(36093..37745)
                     /gene="abc-f"
                     /locus_tag="KPK87_000639"
     CDS             complement(36093..37745)
                     /gene="abc-f"
                     /locus_tag="KPK87_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBG2536831.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(38044..39255)
                     /locus_tag="KPK87_000640"
     CDS             complement(38044..39255)
                     /locus_tag="KPK87_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG2536832.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(39539..40072)
                     /gene="pyrR"
                     /locus_tag="KPK87_000641"
     CDS             complement(39539..40072)
                     /gene="pyrR"
                     /locus_tag="KPK87_000641"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG2536833.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(40432..41379)
                     /locus_tag="KPK87_000642"
     CDS             complement(40432..41379)
                     /locus_tag="KPK87_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2536834.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVIEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(41348..41794)
                     /gene="lspA"
                     /locus_tag="KPK87_000643"
     CDS             complement(41348..41794)
                     /gene="lspA"
                     /locus_tag="KPK87_000643"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG2536835.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
BASE COUNT        13954 a         7154 c         4587 g        16127 t
ORIGIN      
        1 tatgataata agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt
       61 ttgttattta tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag
      121 gtgtgcgtag atggcaaaag tatgtagcgt atgtggtaaa ggtaaggttt ctggaaacca
      181 agtatcacac tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag
      241 agcgattata gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg
      301 taaaattgaa agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta
      361 atttaatcat ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt
      421 aattttattg ttactacttt catcaatact caccccacac tttatagtta aaactatttt
      481 aacaaaaaaa taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa
      541 tccagccagg aagcaacatt gacagaatta cagccattcc tgttgctaga caaagtattc
      601 ctagcaatac cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta
      661 taaatatttt catattcaag tttatgcgac agttttccat tttagaccaa taaacacatt
      721 atgtaatttt aatttttcat actagaatta ttactgaact ccgatataga ttctacaata
      781 tccaaaattc ctttttttct acttaacctt tcaggtccac tatataaaac aactggtata
      841 actttaggaa gtttaaaatt tttatcttct atttcttcaa gacttttttt cataataaaa
      901 cttttccaaa tttgttctct atacgatttc aatcttggga caatttcagt atccatttct
      961 gtttgaaatt ctaacactat ataatgtgat actttgccgc ttttagaaaa taccttataa
     1021 attatatcaa cttcttttcc tttatattca catataaaag atgtttctaa ctctatattt
     1081 ttaagagtta attcttttgc tattgagaca cttgtaaaat tttgaatcaa acttaaaaaa
     1141 ctttctttat ctgaatacat tcttctgtac tcttctcgtt tttcattgct tctttttaaa
     1201 ttcatacagt tcctcctaga ctatagaccc tctatttttt agtaaagata gtctataaat
     1261 tttatttttt aattttcatt agtgagtatt gacagttgtg tgtaaaatat ccagtaaaga
     1321 tattttttta tatttttatt aaaagatata aatttttata ttttaatgta ttgacattta
     1381 cgttgcgtaa aagtgtatat ttaatttata ggaggtacta cttatggaat atacagttca
     1441 aaaattaagt aaactagctg gtataagtac tagaacactt agatattatg atgaaatagg
     1501 tcttcttaaa ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt
     1561 taataaactt caacaaatac ttttttatag agaattagga attaacttag aaaatataaa
     1621 aaacattata aattcaccaa acttcgatag tttaagtgcg ttaaaagaac accataataa
     1681 attactagcc aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc
     1741 tctgaaggag ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa
     1801 gatgattgat gaaaatgaaa aaaattatgg aactgaaatt agagagaaat atggtaaaga
     1861 tattatagat acatcaaata aaaaacttaa aaatatgtct aagcaagatt atgaaaattg
     1921 gcaaaaccta aatatcgaaa taattagtaa acttaaaaaa gcttttaaaa ctggtgatgc
     1981 ctctagtgaa ctggctcagg aagttgcaaa attacatcat aaatggttga gttacacttg
     2041 gaacacatac tctaaagaag ctcattcctc tctagcacag atgtatgtat atgatgaacg
     2101 ttttacatca tattatgata gagaacaacc tggattagct aagtttttaa gggatgctat
     2161 agtaatatat acaagagata aataatataa aataataaag gtggtgtctc aaaatgaact
     2221 ttttagttca tgagacactc ttttttgtat gaaatcagat atatttttat cattttatca
     2281 atgaaaaaca ggtttctctc tctattttga gacagaatct ttattgtata ataattataa
     2341 tcaatacaaa ataataacaa attatacaaa acttacttat actatttatg ctaaaaacaa
     2401 aaaatatttt attaaagttt ttaaaactta tgtaatataa aaatctatac tatagacttg
     2461 attatggaaa gaaaattatc aaaatcatta caattagata tactatctct aatatcattg
     2521 tcatgaatta tctttaaaag gatatcataa atggagttta tttttatata atcatcttta
     2581 tttatagcca acatcatgat taatttaaca gtagaatctt cactccaata tattggttca
     2641 tccaatatgg aaatacaaat cttagttctt atagagctta attccatagg atgaggtata
     2701 gctaaaaaat catctatatt ggtagaagat aaactttctc tgtaaaatac agattcagca
     2761 aatgatggaa atacaatttc actttgagtt aatttgttgc acatcaactt tataagctca
     2821 tctttatctt tgattttagg attaacaata aataaatttt tatcaaagaa attgtccaaa
     2881 agattactat aaatatggga attattagtg attgacttag atatattctc tatatctttt
     2941 tttaatagtt ttaaatccac taatacaata ggaatactat catgatttaa aggaattgtc
     3001 gatattatta tatctacatc tgacaattca ctagctaaaa attgattaaa tgatagacat
     3061 tgaagtatat ttattttatc atgaaaaact ttgtttaatt gaacctctaa taacctactt
     3121 gaaccatacc ctgaaccaca tactaatact acgtttttac acttaatatt actttgaaaa
     3181 aatctctcta ttccagcacc tatatgcagg gatacatatc caatttcatc ttctgttaaa
     3241 gagtaaatac tatttttaaa aactttttct atagcattta aagttatttc aaatgctaaa
     3301 ggataattac tctttattgt atttataagg ggattcacct tatttaaatt ataagattta
     3361 gaattgagaa ttgatttaaa gtgcagtact aaatcatcaa ataatacact atcatttcta
     3421 aggtcaaaag tatattgatt gttgattact tctaaaattt cttcaacata ctctataatt
     3481 ttagtatcta ccctattgga aacgttatcc aaaagagaac ttttagttat aaaatgatta
     3541 tatagataaa ctctgtctgc ctttgaaata attatagtat atttactctc aatataatta
     3601 aatatatttt caacagttgt attcgattga aaatctgtta aaacaacatc taaaacatta
     3661 tctaagcagt aaccatcaag tattctactt acagtaatag ctaaatggat aataaagttt
     3721 tttagattaa aatctaggaa attgatatta aatttattaa tttcacttaa cactaccctt
     3781 tgaagctcta ttaaatctac attattaaat aattctattt ctctatctgt aattccaatt
     3841 atatagtttt caaaattcct atttatcata ttattagaaa tacatcttcg aatatctatt
     3901 tccttacctt caactttaac tccataatat ggcttagaaa ctaatattaa attatacttt
     3961 gacaaaatag gctttattct ttttatgtca tttagtattg taaatttact cacatacaag
     4021 ctattagcta aatcttctag ttttatatat gtattgttat agagaatata ttttaaaata
     4081 tattgatttc tttctatagg agaatctggt atttcacttg aatccataat atcatcagat
     4141 attaatgcta gatactgttg gtataatgct aaattaagaa tatctatata atatccttct
     4201 ccttttttta ttttaataat agcaccattt ttctctaaag ttccatttat agaagtaata
     4261 tcatttctta tagttctctc actcactttt aaatgattac ttaaagtttt acaagtaatt
     4321 ttatcatcac tttcatttaa taatttaaac aacgtgagca atctcttgtg cataaatatc
     4381 cccccttata gaatttcttc taaattatac tatttacact catataatac aattactcta
     4441 ataaaattgt attataataa tttatttcca cttctatatg gaaattagtt attatcataa
     4501 tataaatttc catgtcgaag tggaaattag ttatatttaa tttaattagg ataatgatta
     4561 caatataaat ataagttaag acattatgaa tggagtggaa aaatatatgg tattacaaga
     4621 aaaatatata tttatagata aagacttaaa aagcaaagaa gaagtattaa attttattgc
     4681 agatagtgct tatatactag gtattacaac taataaagag gctcttttaa aagaccttct
     4741 aaaaagagaa aatgaatttc caacaggaat aaaagatgga tttgcgatac ctcatacaaa
     4801 aagtgtaaat gtaaaggaag cttccatact ctatattaaa acaactaacg agttagagtg
     4861 gaaaacttta gatgatagta aggttagata tgtatttagt ttgttagccc cacataaaaa
     4921 tgaaggaaat atacatttga aaactctatc taaattagcc acatgtttaa tggaggatga
     4981 ttttacaagt gaagttaaaa atgctagtga tgcaagtcat ttagtaaaat atataactaa
     5041 taaaatgaag gaggaataat tatgaaaata gtagcaataa catcatgtat agcaggatta
     5101 gcacacacac ctatggcagc aaaagcttta gaacaagctg catcaaaaat gggtcatgaa
     5161 atcaaagtag aacaacaagg tgctatggga cgagtaaata ctatagcaaa agaagaagca
     5221 caagatgcgg actttatact tatagcatct gaccaaacta tatctggaat ggaaagattt
     5281 gatggtaaaa aagtgataaa agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc
     5341 ttaacaaagt gtatagaagc tatttcttaa taaaaaaatc tcactctaat atagaatgag
     5401 aaaaatcaaa aattaaaaaa ttgtctaatg taattataac atattatcaa aaaatataac
     5461 aaacttatat aggaggatta aaaatgagta aaaaaaataa atctctttta agtgaactta
     5521 agggacactt aatgacaggt atctcatata tgttgccact tgtaataggc tcatctttag
     5581 ttgtagctat cccaaagctt acagctttag cagtgggtat aacaagttta gatgcttata
     5641 aaaatgtatc tggtttttat catattcttt acttaatgga acaagttggt tggactggca
     5701 taggtctatt aaatacggta ttagcaggat ttatagcatt ttcaataggt gagaaaccag
     5761 ctatgggtgc aggttttatt ggtgggctca tagcaagtaa tactaatgca ggattcttag
     5821 gagcagtaat agcaggtttc tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta
     5881 aaatatctgg gtcagcatca ggaatgatgc cacttataat aatgccactt ataacagttg
     5941 gattaactgg ttttttaatg agtgttgtat tagcaggacc tttaggaaac ataaatactt
     6001 cattaaatgc ttgggtagct gaaatgtgtc aaaatggaac aaattcggta gtattggctt
     6061 taattttagg tgcaatgata ggattcgatt taggtggacc agtaaataaa gcagcttgga
     6121 tggcaggaaa tgcattattg ttagagggaa tctatctacc agcgatattg gtaaactgcg
     6181 caatagttat acctccacta ggatatggtt tagctacatt cataagaaaa tcaaaatttt
     6241 ccaatgcatt agctgaaaca ggaaaaggca atatagttat gggcataatt ggaataacag
     6301 aaggtgcaat tcctttcact ttaacaaatc cattaaaatt agttcctgtt aatatgatgg
     6361 gatgcgcttt gggtgtagga ttatcagctt tgttaggtgt acatgctata atgcctccag
     6421 ttggtggatt atatggattt atatcaatag gttctggatg ggcttactta gtaggtgcaa
     6481 tatttggagc tttagtaata gcaatattat caacaacttt agtagacttc aatgaagatg
     6541 aaactacgga aaatacagaa tttgatgaaa tagagttaga tataatgtaa attaaaaact
     6601 taattttaga taattgagaa ttgagtgtac tctcaattct caattaaatt ttgggaggat
     6661 ttttatgaaa aagacaaaag tacatgtagt tccacacagt cattgggata gagaatggta
     6721 ttttacaaca tcaaggtcaa aaatatattt aatgaaagat tttcaagata tattagacat
     6781 attggaagaa aaagaagact ttaaatactt tactatggat gcacaagctt ctctcttaga
     6841 tgattattta aaatggagac ctcaggatga atatagaata agagatttag ttcaaagaag
     6901 aagattagtt attggacctt ggtatactca aacagaccaa ttggttatat caggagaatc
     6961 tataattaga aacctttact atggaataga tagatgcaat gaatttggtg aacctatgag
     7021 ggttggatat gtaccagatt cttttgggca atcagctcaa atgccacaaa tatacagaag
     7081 ttttggaata gatagttcat tattttggcg tggagtttct gatgatatgg tagatacaac
     7141 agagtttata tggaaaggtt ctgatggttc aaaagtatta gcagttcaaa taccatttgg
     7201 ttattactat ggaggtaata taccagaaga tgattcagat ttaaaagaat atttaaaaga
     7261 tataatcgga aaacttaagg ttaaagcttc tactaataac gtatattttc caaatggatt
     7321 tgaccaagca ccaataagaa aaaaccttcc tgatatatta aaaaaagcta atgaaataga
     7381 taatgaaaat gaatacgaaa taagtgatat agaaaactat atagattcag taagaaatga
     7441 aagaaatgaa tttacagagc ttaaaggtga atttttaaat gctaaacata tgagaataca
     7501 caaatccata ttctcctcaa ggtcagacct taaaataatg aataacaaat tagaaaatta
     7561 tattacaaat gtacttgaac cattattaac attaagttat tcacttggaa atgaataccc
     7621 tcacctagtt ataaaagata tatggaaact tatgtttgaa aatgcagctc atgacagcat
     7681 aggttcttgt aatagtgaca caacaaatga ggatgtatac acaagataca aacaagccag
     7741 agatttgagt ataaatttat tagatttgca tatgagactt ataagtactc aaatcaaaaa
     7801 taataatgaa gaaataacat taacagtatt caatggattc ccagaagtca gaaatgaagt
     7861 tgtagaattt gatacttata ttccaggtca agattttgtg ataaagaata aaaatggaga
     7921 aattcttaat tatataataa aagaaaaaga agatattaca aattatatat taactcaaac
     7981 aataagatta aacccaagta aaaaaatata tatacccaaa actgtatata gagcaaaaat
     8041 tgcactttca gtaaaagata taccaccaat gggatatact caactagttt ttgaattgga
     8101 tactaaaaat aaatgtgaaa tagaaacaag taattgcaaa gaaatagaaa acaaatacta
     8161 caaaataaca gccaataaaa atggttcatt agaaatatta gataaagaat ctaataaaac
     8221 ttataaaaac caagctgtaa ttgaagataa tggagatgat ggagattcat acaactattc
     8281 tcctccaaga aaagatattt atgtttcatc tttagattct gtatcttcag tagaagtgct
     8341 aaaatcaaac attcaagaag aaatgatact taaatattca ttgaccatac cagcttcact
     8401 ggaagaaagg gctataggta aagtttctgt gaaaatgcca gtaacaatga aaattacctt
     8461 agaacctaaa gaacaaataa ttaagtttaa tgttaatata aaaaataacc aagcactatc
     8521 tcatagagta agagttttat ttaatacaga aatagcatct aagttttcta tagcagatca
     8581 acaatttgga ataatacaaa gaccaacagt attagaaaaa gaccttgcat tgtggaaaag
     8641 agacaattac tcatggcaag aaaaacctat aactatcgaa ccaatgcaaa gttttgttac
     8701 tttagaggat ggacaaagag gaatagcatt aataactggt tgtgtaagag aatatcaaat
     8761 agttggtgat aatttagata taatagcatt aacactattc aggtcatttg gatatatggg
     8821 aagagaaaat ttactttata gaccaggaag agcttctgga gaaaaaataa tagcaacacc
     8881 agatgcacaa ttacttaaag agttaaactt tgactttggt ctttacatat ataacaataa
     8941 atttgatgaa gcaaatgtag ctaatacagc taagagattc ttaacaccta tacaaatata
     9001 tgaatatgct gattttttaa atggtagact tatatttgca ttcaatgacg aaaaacaaat
     9061 atatgaaaat gaatttagtt tgatgaatgt aaataattca aatttcacag taagtgcaat
     9121 aaagaaagaa gaaaaaggag atggaattgt agttagaatc ttcaatggta tgaaaaatga
     9181 ggatgctaag ggaagtctta acatcaataa aaatgtcaat gatgcttata gtgcaatgct
     9241 cgacgaaatc tatgataata ctagcaagct taaagtaaat actaaaactg tagaggtaaa
     9301 ttctttatca cattgtaaag tacaaacaat agtaattaaa taatataaaa tagcttatac
     9361 aagatttcaa aataaattgc tagtactatg ttaatagtaa agccagatat tatgctattt
     9421 ataagctctg taacactaaa taacattttg taaaataaaa aggtgaacct tgtatattag
     9481 gattcacctt ttttaaataa atcaacattc tgttttaaaa taacatttat aagctgtttt
     9541 gaaataatgc ttacaaatta ttttaaaaca atacctataa atttttattt aaagaataca
     9601 cataaatatt tccagactct tttatcctat agactttata attattaaag aagtctaatc
     9661 taatttttgt taaaagtaat tgtaacttta aataaatcac catccacact tatattcatc
     9721 ttaccaccct gtaattctat aaaacttttt gctattgcta aaccaagacc agaaccttct
     9781 gtgttacgtg atttatctcc tcttacaaat ctctccatta tatcatctac actaaaatca
     9841 atctcagatg cagacatatt tttaagcata atatttactt tattatctgt ttctaaaata
     9901 tcaatataca ctcttgaatt tggcattgca tatttgctta tatttattat aagattttca
     9961 aatactctaa atgtacgctg actatctaat ggtaatatta ttttttcatt agaaaagtta
    10021 ttctttacta ttagagatga atctgcaagt ttgtcatcta attctaaaag tgtctgcttc
    10081 attagagata caatatccac atttactata ttcaaataaa catctccact atttgccttg
    10141 cttacttcaa acaaatcttc tattagatgt tgcaatcttt gtgactttct gtctaatgta
    10201 tcaatataca acttacgatt ttcatctgtt atactttcat cttttaataa atctatataa
    10261 gtgattatag atgttagagg tgtttttaag tcatgtgata catttgatat aagctctgtt
    10321 ttcatctttt gactcttaac ctcttcgtca acagcttttt taaatccagt ttgaatattt
    10381 cctaattcgt cttttatagg attaaaaaat cctaaatctt tttccatatt tacatctaag
    10441 tttccatctg aaattttctt tgtaacatct aaaagttgat tatattcata tattgtcttt
    10501 ttagagtaat ctgacagtat cttaaataat actactgaat atattaatgc gagtattatt
    10561 ccaaagaacc acatactaca cattataact ataatcaata aatttattcc caaaattgta
    10621 actatagttt tatttggctt ttcttttaga tttattgttc taatttgatt aataactgtt
    10681 tttcctgctt tttttagcca tacaaaaaac attcctgtca cagtatgttt tgtaaaatac
    10741 tctcttattc caacatttaa aatatgtttt aaaagtatta taaatacaaa tattattgag
    10801 aatattacaa accaataagt taaattcatt aaacttacca atatactagc cattttagca
    10861 cttatatcat tgtttataag gtactctaca taattcccac tcaaggtatt acttattata
    10921 acagtaggat ataaatctac aagcccctgt ataatagcaa gagcaataaa gagtatctct
    10981 aacggtattc ttgataatgt atcaaatcct agcaactctt tagattttct atatgggaat
    11041 gctaaagcta caataacaac tatagctaat ataattttta aaatcattcc agaaatctta
    11101 taataagaat tattttctac attgcttata tatgaaatta taccatcatc aaaataagta
    11161 tttccataac catactctac actatcacct ttacttatta ctttaggtac agcatatact
    11221 aatgttatat tttttattgg agataactta tatgaaattg aatttcctcc taaacttgta
    11281 tccattaaat cttctcttgc tttagataac aacatttgat taataacttt ttgatttact
    11341 ccataagaat ccacaatatt tacttttcca tctttatcaa aattcattac aacgtagaat
    11401 ctatagtttt cttttatatc tgtaatttgc ttttgtcttt cctcatcatt catttctgta
    11461 ttttttgact gattttcatc agaattttct tttgaagcat cattctttgt tgtatcaagt
    11521 actcctaatt ctttattatc attaaaaaat tcaaaattgc tttctttatc aactaaatag
    11581 tactttaaat tctttcctga cgctttaatg aaatgctccc aattttcaaa gttttcatta
    11641 aaatctgcat taatactatc atctgtataa taagtttctg aaccatcata agtatctttt
    11701 tctttactta cattttttat atagttcata gtttcttcat tgataaatag ctttgatggt
    11761 tgtatcatct taccttcttg ttcttccttc atattatgat ataaaacata acttccttta
    11821 tatatactat ctacaaagct ttcctgttca aatggattct ttgctccacc tttagcacct
    11881 gcttgtatag tagaatattg actaatcata cctaatgatg ctgataacat aagtataaaa
    11941 actataatag taactatata tttatcttta aattttttca attttgtatc caacacccca
    12001 caccaccttt aaatatttag gattttttgg gtcaatttct atcttttccc ttatattcct
    12061 cacatgtacc ataactgtat ctgtatttac agcattctca ttccaaactc tttcatatat
    12121 ttcatcagca gaaaaaactc ttcctgcatt tctcataagt aaacttagta ttttaaattc
    12181 tataggagtt gtttttatat actttccatc aatactaact tcttttgtat tttcatttaa
    12241 ttctaatcca cctattgcaa acacactatc gtcactttct accttttgct ctttatttcc
    12301 aaccatgttt agatacttag tgtaccttct aagttgtgaa ttaactctag ccaatagttc
    12361 taatggctta aaaggttttg ttacataatc gtctgctcct atattaagcc ccattatttt
    12421 atcaacttct tctgattttg cagatagcat tattactgga aaattatagt tttctctaag
    12481 cttcattgta agtgttatac catccatttt aggcatcatt atatcaacta tagctaaatg
    12541 tatttcttgg ttttctataa ccttaagacc ttctaatcca ttgtatccct taaaaacatt
    12601 atatccttga tttaatagat atatttctat agcatctgct atctcctttt catcttctac
    12661 caccaaaata ttatatgaat tcattctaat ctcctttata ttaatttata aattttcttt
    12721 acatatatga ttatacaata tgatagaagt ttttccattt atatatatat ttatatcttt
    12781 tgttctggtt atttgtattt aatgtccatc ttagaaacct ccttctagtt taaatttaat
    12841 attttttcat tttgttttat atacttaata ttaaaattaa aaacttaaga tattacatat
    12901 acttttctaa agattttcta aagatttaaa tatatttaaa attaatatac aaaaaaaagt
    12961 agttcttatt gtattaatta aattaatgat ttgaaatata aaattaatat caataaaacc
    13021 tactttttaa ttttttaaat atttattttt aatttatttt gtttttaatt tacttcgatt
    13081 ttaaaatgtc cttgactttt attttataaa tgtacatatt tcttctactg gttttttaga
    13141 tggactcttc aaattgtcct ctggcactcc aagagctaac attgcaccta aaaaatactc
    13201 tcccttttca aaaccagttt ctgcttctaa aacagcttct atctcatctg ctgcatagtt
    13261 ttgacttgtc aaccaacaag aaccatatcc aagttcaatt gctgatagtg taaagttttc
    13321 aattgcagca cctagactct gcatacctgg atttcttata aataatttat ctatagtctc
    13381 cttaggtgca tctataagct ctaattcata atatcctgat ggattatata cctttgtaaa
    13441 aactaatact aaaactggag cttttaagta aaatagtgta aaatttttaa caaattttct
    13501 aaatctatta gccttatcca ctgatacttt atccatttct acaagtattt cttgttgctt
    13561 tttagttatc acatctgcta tcttttccat taaatcacgg cgttttataa caacaaaatg
    13621 ccagttttga atattcttgc ctgatggtgc tgcacctgct gcttttatca tttttaaaat
    13681 atcttcatct gatacatctt gatttttaaa ttttcttaca ctttgtcttt taaaaatagt
    13741 atcttgtaat tccattttta aatcccccca taactttttt attattttaa tataattttt
    13801 aaactcatct tattttaata tatcatgttc taacaaatct ttaaatgagt ctataaaata
    13861 atcatataca tctacattcc caaacccata tttggcatat ataaaaggta tacctacaaa
    13921 cttagcacta tttgcatcac cttgcgtatc accaacatag attggattta ttaatttatt
    13981 tctttcaatt ataagtttat tattttctcc tttatgcaaa ccagtattac caggacattc
    14041 aaaatctata aaatactgtt ctagtttatg tgcttttaaa aaagattcta tatatccagc
    14101 ttgacaatta cttactataa ataatttata ttttttagat agtaatttaa gagtatcttc
    14161 tatatcatca aataatttag ctccattttc agataaatag tcacattctt tcaaagaaca
    14221 ttcattcaat aaattcattc tttcttcttc acttaaattt ggaaataaac gatttgctat
    14281 ttcctcaaaa gaaagcccca taagacttct aaataaatct tttgtgacac ttaattctat
    14341 ttccttatgc tcacttaaaa catcctgcca gactttgcat acttcttcag ttgaatccca
    14401 caatgttcca tctaaatcaa aaataatact atctaccaca aaaaatcccc cttatgtcaa
    14461 agtttattat ctatttataa ttatatacta aaaagcagat acaatcataa atatatttta
    14521 cttgattgta tctgctctac aatataaaac caattgtcaa aatcaaattt tataaatttc
    14581 taactacaag tacacagcct tgctctactt taatcttaca agaattactc aacataacat
    14641 ttgatattcc aataggtaca gagtatttca tataataatt atcgagaggg tattctaagc
    14701 cagtaagtgt tacaccctta gcatcaccat ttatagctat tacagaaata gtgtcccctg
    14761 ggtttccata caatgaaatt tcctcatttt caactatata catctcttct ttatcagaaa
    14821 gtatcctaga atatatacca tcttctttta aataatataa tagctttata tttgcaagag
    14881 tgtggtcaat ccttccccct aaagcaccaa aaaaatcaat atgatttgct ttaagtgtct
    14941 tagctaaaaa aagacaaagc tctgtatcag tctcatcttt tttagatgga aatttttcaa
    15001 atttaacacc tttattttta tagaagttta tcttttcttc ttctactgaa tctaaatctc
    15061 ctaatatata atctggcatt atttccattt tataagtgtg atttgcccct ccatctgcac
    15121 atattatgca gtcataacat tccttaatta taatatctct tgtaacttcg taatcttcaa
    15181 tttcgccatt taaaacaata cagattttca ttattaaatt taaactaatt cttcaagaga
    15241 agcattcttt ctaaataaat taactgtttc ttgaatgtca tcacttccaa atattgcaga
    15301 acctgctact attacattag ctcctgcttg tacaacttcg gctacgtttt tagggcttat
    15361 accaccatca acttctatat caatgtttaa tccttgacta tctataatag cttttaactc
    15421 tttaatctta tctaaaacac aaggaataaa tgattgtcct ccaaatccag ggttaacaga
    15481 cattaataat accatatcta aatcaggtag tacatgttta atcgtatcta ctggtgtagc
    15541 tggattaagt actacaccag cttttattcc atgtgatttt atattttgta tagttctatg
    15601 tagatgaaca caagcctcct ggtgaacagt tattatatca catcctgctt ttacaaattc
    15661 ttcaatatac tggtctggat tttctatcat aagatgagca tcaaatacca tatttatatt
    15721 ttctttcttt aaacttttga ctacaagtgg tcctaatgta atgtttggta caaagtgtcc
    15781 atccattaca tcgatatgaa gatattcaca tcctgcactt tctacttttc ttacatcttc
    15841 taataatttt gcaaaatctg ctgataatat tgatggtgct aatttaatca tgtttctaat
    15901 acctcctact attattttgt cttatttcat gtagcagctg tatataacta tcatatcttt
    15961 ctttagatat ttctccattt gtaacagctt cttttatagc acaattaggt tcattttcat
    16021 gaatacactt tgaaccaaac ttacaatcat taaatttatc aaattctata aaataatctt
    16081 taagttcaac ttcctctata tcttcaaggg caagggaact aaaacctggt gtatctgcaa
    16141 ccattccacc aaattctaat tttaaaagtt cagcgtgacg agtagtatgt tttccccttt
    16201 ttattttatc acttacaact cctgtttgaa gtttaaaatt ttcatctatt tcatttaata
    16261 agcttgattt tccaactcca gatggtcctg caaacactac tacattttct tttaattctt
    16321 cttttacttt atctatattt aacttagtaa tattacttac aggaatgact ttatagccac
    16381 taagttcata tatattttta acagtttcta atgtatcatt atcatctaaa tctgcttttg
    16441 taaggattat tacagtttct aaattttctt tttctgccaa aactataaat ctatctaaaa
    16501 gagataagtt tggtttagga ttttttattg cgaatactat taatgcttta tctatatttg
    16561 caataggagg tcttataagt tctgtatctc tactgtcgat ttcttctaag ataccctttt
    16621 tttcatcttc atcaacaatg cttattttga ctctatcacc aaccaaaggt gttattttct
    16681 gctttctaaa tatccctcta gccttacatt cataaatacc attataagta tctacataat
    16741 aaaatccact aatccccttt ataatttttc cttctagcat caaatgcctc cctttataaa
    16801 atttatataa acacatactg tttatgagta gtatatcgtg ttattctttc ctatttttat
    16861 ataataccac atttttttta aataacaaat cctccgtttg gactaattac ctgaccagtg
    16921 ataaaatttg atttgtctga tgccaagaaa atagcacaat ttgctatatc ttctggagtt
    16981 cctagcctca ttagaggggt ttcttccaca agagcgtcta tatcttcctc attatatcca
    17041 gataacatgt cagtattaat gacacctgga gctatgctat ttactctaat atttgatggt
    17101 cctacttctt tagctaatgc ctttgtcatg cctattatgc ctgcttttgt tatagaataa
    17161 tgcacttcac aagaggcacc tgatataccc cagattgaag atatattaat tatatttcct
    17221 tttttttctg atatcatgta ttttaaagca acttgtgaac aataaaagct tccttttaaa
    17281 ttaatattca tcatattatc ccaatcttca tcagttatat ctgtaaacaa cttatcttga
    17341 cttacaccag cattgtttac aagtacatcc aatcctccaa attcttttat acaataatct
    17401 accatatcct ctacatcttc tctatttgaa atattagcct taaaaagttt tacagaaaaa
    17461 tttttttcat ttaatatagt gtacaactct ttagcttcat tttctgactt attaaaattt
    17521 actaatacat tgtaaccttc ttttgcaaaa gctttagaca tagctttacc aatccctcta
    17581 gctcctcctg taattaaaac tgttttcttc ttcataaata cttttctcct tatattataa
    17641 ccaattcagt attttataca aaacaaactg ccctttaaag aaaatatttt ctataaaagg
    17701 acagctctta ttttattaat atttattctg ttttaccaat gtttgcgcca gttcctgatg
    17761 aagttcctcc attatctcct gagtttgagc ctcctgaact atctccattg tctcctgagt
    17821 ttgaacctcc tgaacttcct ccattgtctc ctgagtttga gcctcctgaa cttcctccat
    17881 tatctcctga gtttgagcct cctgagtttc caccactatt tcctgagttt gaacctcctg
    17941 agttacttcc attattgcct gagtttccac cactatttcc tgaatttgag cctcctgaat
    18001 ttccaccact gtttcctgag tttgaaccag gttctgttgg tgtattattc tctggtgttg
    18061 ttggtttttt atctggtact gttggagtct gtgtatttga tttttctgaa cctttgctta
    18121 catataggtt tacctcatca ccttcctgag ctcttgatga gccagcaccc ggactctgac
    18181 ttaaaactgt accttcttta tatattgagc tgtattcata cttaacagtt cccactttta
    18241 agttattttc ttcaataagt tgttttgcat tactcaaagt aagtcccact acattaggaa
    18301 cagatgcctg acttgaacct ttacttacga caacatttat agtatcaccc tttttgacat
    18361 ttgtacttcc accttcaggt gtctgtgata ttatacaatt ttcatcaact tcactattat
    18421 actcttcact ttttacactc aggtataatc cttctttttc aacagcactt tgagcttctt
    18481 ctaatgtcat atttacaaga ttaggaacag tcaaagattt attgccaaac cctccagcaa
    18541 ataaaaactt atatgctaag aatacttgag cacacaatat cagtatcaaa acagccgcaa
    18601 ttacttttaa tcttcttcta gattttgggc tttcctgttt tttcttcttt ttcttagttc
    18661 tcttacttgg agtactctta ggtctctgat tctttttagc tctcattatc tcttcctctt
    18721 cttcatcttc atcatcatct tcttcataaa aatcatcata gtaatcttca tcatcatcca
    18781 aatcagctac ttcaacaggc ttaaccactt tttcaggagc tggttttgca agtgttggat
    18841 ttacaacttt attaatttcc ttttcatcaa ttttcttagt tgcaaaatca tcatattctt
    18901 ttataaaatc taaatcaata tttttttcta tatactctat atcttctatc aattcttcag
    18961 cagtttgata tctatctgca ctagattttt ctgtaagctt ttttattgta gttcttacac
    19021 tttgaggaat cctaactttt tcttctgatg taaagtctat atcatcatta atgtgttgaa
    19081 gtgctataga aattggacta tctcccctaa atggaacttt tcctatcaac atttcatata
    19141 aaactatacc tagagaatac aggtcagcat tattagttac aaatttacct ttagcttgct
    19201 ctggtgaaaa ataatgcact gaacctatta tactaccaat attagtcatt gttgaatttg
    19261 aaacagcttt ggctatacca aaatcagcca cttttactac tcttccttca tttgatatga
    19321 gaatattatg aggttttata tctctatgta taatcccctt tttgtgcgct gcactaagtg
    19381 ccatagctat ttgttttgtt atatctaatg cagtatattc atctaatata ccttcatctt
    19441 gtataatctc ttttagattc ttcccatcta caaactccat tacaatgtag tgaacttttc
    19501 catcttcacc aacatcataa acatttacta tatttggatg tgaaagactt gctactgctt
    19561 ctgcctctct tttaaacttt gttagaaatt catcatcatc tacaaattca ggtctaagta
    19621 ctttaagtgc aacagtcctg tttaataatc tgtctttagc ctcatataca aaggccattc
    19681 ctccatcacc aatcttcctg atgatttcat atcgatttcc taaaattgta tctcccacaa
    19741 tatcaccgtc ctaattatac ttttatcact ataaccgaaa cattatcttt accagaaaca
    19801 tcattggcca tacttatcaa ttcttcactt atattactag ttctctcata ttcataatct
    19861 aatattaaat ctcttatatc ttcatcatca ataaacccag ttaatccatc tgtcgcaaga
    19921 agtattatat cacttttttt gatttctttc ttaaaaatat ctacaacaac catatcatct
    19981 gttccaattg ctctagttat tctattcctc tgtggatgtc ttcttgcctc ttcttctgta
    20041 atgacatggg ccatcataag ttcttcaacc acggaatggt ctatagttat tttatcaaat
    20101 ttttcatctg ttaacaaata acatctgcta tctccaacat ttgccacata taagacatta
    20161 ttatacacta tagccgtaac taatgtagtt cccattcctt caaattctat atcttccata
    20221 gattttttgt gcacaattga gtttacatta ttataagcct gttttaatat atcatctata
    20281 tagtctattt ttacattatc atgttgtaat aagttttcct ttagaaaatc aattatattt
    20341 tctactgcta atttactggc gacttcacct tttttatgtc cacccattcc atcagctatg
    20401 gcaaatatcc ctattggacc atgttctgta tctataactt ctccctcaca atagtcttca
    20461 ttgttttttc ttatttttcc tatatgggag gcacaactat aaaccatacg acttcctcct
    20521 tacaaattac ttgtattttc ttctcaattg cccacaagct ccactaatat cagaacccat
    20581 agacatccta actgtggcag gtatattgtt cttctccaaa ctatccctaa atttataaat
    20641 aaatgcttta tctggttttt cgtattctct ttcttcaact ttatttattg gaattaaatt
    20701 tacatgacat agcatacctt ttaggagctt agctaatgcc tttgcttctt tttctgagtc
    20761 atttacccct tttataagag aatattcaaa tgtaaccctt ctatttgtct tttttatata
    20821 atatctacat gcattcaata tttcttcaat agaataagca tttgctacag gcataatttt
    20881 tcttcgttct tcatcatatg gtgaatgaag tgataaagct aaatttatag ctatttctaa
    20941 gtctgccaac tcatacattt taggtactat tccacaagtt gaaagagtta tatgtctata
    21001 ccctatatta agaccatttt tttcgttaat tatttttaaa aattgttttg tattttcaaa
    21061 gttatcaagt ggctcaccac tccccatcaa tacaagattt gataccctct taccagtatc
    21121 ttcttgaatt ttaataactt ggtctaaaat ttcccatggc tctaagtttc taattaatcc
    21181 atccattgta gatgcacaaa aattacatcc catcctacaa cctacttggt tagaaacaca
    21241 gacagtaact ctactatcat aatccatcat cacagtttct attatatttc catcatctaa
    21301 taaaaacaaa tatttttttg tattatctac cttagattct aactttaatt ctatatctat
    21361 atgccctata catgaaacct cttctaattt atttctcaaa ctttttggta tattgttcat
    21421 atcatcaaaa gtttttgcac ccttatatat ccaagagaat atttgacttc ctctaaaggg
    21481 cttttcatct atagttttca taaattcttt aagttcatct tcagtaaaat tttttaatac
    21541 tatctttttc ttttccattc tctcactacc ttattctctt taattttgct atgaaaaaac
    21601 catctatacc atgaatatta ggatatatct ttaaatatcc tttatcttga ttgtctaagt
    21661 ctacatttac tgtatcaatt ggaacaaatt caaagttgtg attttcttca acaaaagaag
    21721 taactatatt tatattctcc acatcttgaa ttgtacatgt actatatact aaagttccac
    21781 caactttgac atatttagat gcattttcta aaattttatt ttgtatagaa gtgatgtctt
    21841 ttaactcttc ttctttctta tacttaattt ctggtttacg tcttattata ccaaaaccag
    21901 agcaaggaac atctgctaaa acataatcaa atttacttat actattttca tcaatctcag
    21961 aagcatcaaa tctttctaca aacacatttt taagtcccaa tctatttaca gtagagttta
    22021 taagctttag tttatgctca aaaatatctc tagctactac ttgaccagta ttgttcatta
    22081 aagtagctaa gtgagtagat ttccctccag gagcactaca tacatccaaa acaaatgaac
    22141 cttcttttgg attaattact ttccctacta tcatagaact tatatcttga attgtaaata
    22201 aaccagcctt aaataattca tttttttcta tatttttcat tttttcgact ttaatagctt
    22261 cttctaccat aggaactttt aaacatacaa tacccatttc acttaatttt tcaataagct
    22321 cttctctact tattttaagt gtattcgttc ttatatagat actaggcttt tcattatttg
    22381 cttccaacaa atcttcagca aattcttgtc caaatttatc tatccagttt tttataatcc
    22441 aagagttata ggaatacttt atagataagt attttacact atcttcctca gttatgtcca
    22501 ttatagtctc tttagctctt atttcatttc taagaatagc atttatgaaa ccagaagatt
    22561 ttttatcata tttcttagat aattttacag tttcattaac tgctgcataa tctgatatac
    22621 tatctaaaaa taaaatctga tatgttccca ttcttaaaaa gattttaaca taagttgaca
    22681 ttttttttac ctttattttt gaaagtttat tgattatata atctaaataa tatttatttt
    22741 ctattactcc ataaattaac tctgttgcaa gtcctctatc catatcactt atttctaaat
    22801 ttttaaaatg cttatttatt gcaatatttg aataattgtc atttttttct atatcacata
    22861 atactttaaa tccaacttct cttgcatcca tataaatttc tcccttttat taaacataaa
    22921 aaagggttgt ttctaaacaa cccactttac ttttagtcat ttcttctttg agctattagt
    22981 aacaatctaa gtaattgaag tattgaaacc aatgctgctg caacatatgt taaagctgct
    23041 gctgaaagta ctcttctact ttgtcttgac tcactttcat ctataatccc taaattccca
    23101 agttgaacga ttgctctact tgaagcatta aattctacag gcaatgttat tatttggaat
    23161 aacactgaag ccgaaaacaa tagtatacca atttttaaaa atggaccagc cataataaat
    23221 cctaaaaata ttaaaaacca agatatactt gaagcaaaat tcactactgg tactaaacta
    23281 cttcttattt gaagaggtgc ataaccttta gcatgttgta tggcatgacc acactcatgt
    23341 gcagctactg caacagaagt tatagatgtt ccataatata catcttctga caaccttaca
    23401 gcctttcttc ttgggtcata atgatcactt agatgccctc taaccatttc tatctttaca
    23461 tcatacaaac catttgaatc aagtactctt cttgctgttt gctctccagt atatcctcga
    23521 cgtgtattta ctcttaaata cttatttgtt gttgaactca ctttaaattg tgcatatata
    23581 gtaagtaata tagctggaat taatacaacc attgttgggt caaatcccca aaaaccgcca
    23641 tatacagggt acataaataa cactctccta aaatatattt ttattataca tcctcttaat
    23701 attcacttaa cttaaaaaca gtttaattac aaactaatat attttttcct gttaaaaagc
    23761 ctatagcttt ttcaatatcc atcatgtcta cattagtatt tacacaaggt ccattaggtc
    23821 ttttgttaaa tacacctaaa acaggaattt ttttaatatc ttgtattcca gatgtcaaat
    23881 ctctttcaca tgcaacagct acaacagctt taggtttagt gtccattata atttttctag
    23941 ctagagttcc tcctgtagct acaaatattt ttacattagt tttttccttt agcttaatta
    24001 attctcctat gttgcatctt ccacattctt tacaattatc tatatcatta gtaactttta
    24061 atttacaact attctcttga atacaatgag gtattaaaat tattatatcc tctggattaa
    24121 aattatattt attactgtat atatattcat tatttaactt tatatatatt cttctaatat
    24181 cactttttga cataccaaat gatgatgcaa tgaaagatat aataggaaat aatccactga
    24241 caatattaaa atttattttc attatatttg agttaacata ctttccttta attactctat
    24301 aagtaactgc tgttgaacat agtattataa gtattagtga tattattata atatttatca
    24361 tcaaggagaa tatttgagtt aaacttatta taaaaaaatt aacaactcct atcaaaattc
    24421 ctaatattaa aatcaaaatc ccaacacttg ttaaatactt ctttatatct gtcatacttt
    24481 tcacctaacc caatactaaa ccagtttcta tagtattccc ttttatatat tcaccaacca
    24541 acattctctt tttattcggc atctgaattt cttcaattaa aagtacatta tctatagtac
    24601 ttacttttat cccatctttg cttacatcaa ttattgtacc tacatctttt gtgctttttt
    24661 catctaacac tttagttttc caaactttca ttatgacatc attataggta gtataagcac
    24721 ttggccatgg atttactcct ctaactaaat tgtgtatttc tctagcactt tttgaaaaat
    24781 ctatgttacc taatgattta ttcattattg gcgcatagga aaattcttca tgattttgta
    24841 cttctcttgg agcatttcct tcttcaatta atcttaatgt ttcttttaaa gtttctgcgc
    24901 ctatattcat cattttatca tgaagttctc ctgctgttat attttcatca agatttactt
    24961 ctgtctttag tatcatatct ccagtatcta gcccttcatc catatacata gttgtaacac
    25021 cagtcttttc ttcaccattt attattaccc aatttatagg tgccgcacct ctatattttg
    25081 gaagtaaaga aacatgaaca tttatacatc caagtttagg aatctctaat atttcttttg
    25141 gaagtatctg tccaaaagct acaactacta ttacatctgg atttaaagat ttaattttat
    25201 ctataaattc tttatctcta gcctttactg gttgatatac aggtatatta ttttcaattg
    25261 ccagttcttt tactggactc atacctagtt ttttacctct tccttttggt ttatctggtt
    25321 gagttacaac acccaatatt tcatacttct catctattat tttttgtaaa catggaactg
    25381 ctatatcagg agttcccata aatactattt tcatcaaatc aactcctact acttttctat
    25441 tttgtcaaca aataaaattc catctaagtg gtctatctca tgacaaaatg ctctagctaa
    25501 aagttcttct ccctctaact ctattgtttc accatttcta tttaaagctc tgacctttac
    25561 ataatttggt cttctaacat ttcccgcttc tccaacaaca cttaaacatc cttcctcatc
    25621 tatttgctcc ccagatgttt ctattatttc aggatttata agttctataa gctcttctcc
    25681 aatatctatt actactactc tttttaatat tccaacttga ggtgcagcca gtccaacacc
    25741 atctgcatca tacatagttt ctgccatatc atctaataat tgtattatct tttcatcaat
    25801 tttttcaacc ttttttgatt tttttcttaa tactggttct cctatttgaa ctatttgtct
    25861 taaagccatt ataattcctc ctaaatttat aatacactgt ttggattaat atctatagat
    25921 acattgatat tcttatcaaa tatcaaatct cgttttgtta tacatatata ttttattata
    25981 ccctttaaga gattaatttc aatattttca tctttaaaga gtatttgcca tctataattt
    26041 tgatttattt ttgatattga acatggattt ggacctaata taaatcctaa atcatttata
    26101 cccctttttt ctaataaata aatcaatgat gcatgcatat tttttatatt ttttatcact
    26161 aatctttcat cttcaccact aacaactaca cttaacatat tattaaaagg agaatatcca
    26221 aatgcttttc ttatttttat ctcatcttca taaaaaccct cataatcata ttcaatagca
    26281 tgcttaatag catagtgttc agtgtcataa gtctgaagta ctacttttcc ctctttatca
    26341 gctctccctg ctcttccaga gacttgagtt atcaattgaa atgtagtttc aaaactttta
    26401 aagtctggaa aatttaatat catgtctgca gacaaaatac ctactaaagt tacattctca
    26461 aaatccagcc ctttacttaa catctgtgtt ccaataagta catctgcttc tttatcttta
    26521 aatttattta gtatctcatc taaagcacct ttttttgaag ttgtatcttt atccattctc
    26581 aaagttttta tatctggaaa aatatatttt aattcctctt caattttttg agttccaact
    26641 ccaaatggct taacataagt acttttacat tcaggacact cttttggaat ttctttctca
    26701 tatccacaat agtggcatct tccagtattg cttttcttat gataagttaa agatatatca
    26761 caattttcac attgaaacac atatccacac tttctacaag atacaaaatt tgcataacct
    26821 cttctgttta aaaatagtat tacttgatta ttttcttcaa tcgcataacg tatttctttt
    26881 tgtaatttca aactaaatat acttctattt cctttatcta actcatcttt catgtctacc
    26941 acttcaatgt ttggaagagg cttatcattt gctctacttt ttatatttat aagtttgtat
    27001 tctccagatt ttgccctata atactcttca attgagggcg ttgctgaacc taaaataaga
    27061 gttatattat tttttaatgc cataaatcta gcaacttcta tagcattaaa ttttggattc
    27121 ttctcagact tatatgatga ctcatgacat tcatcaatta ttattaagcc taaggaatta
    27181 aaaggtgcaa ataaagctga cctagctcca attaacactc ttactttacc tgctttgaca
    27241 gctttgtaaa catcatgttt ttctccttca gatagtttac tgtggaacac tccaactaaa
    27301 tctccaaacc tactctttag cctatctatt gtttgaggag ttaatgctat ctcaggtact
    27361 aagaatatac tgtctaagcc ttgatttaga gcaaattcta ttatttccat gtaaacttca
    27421 gtttttccac ttccagttac tccatgaatt aagtatggtt ttttatcatc tacaaacata
    27481 ttcgacttta tctcatcaac tgcttcttgt tgctcattgt ttaattttat actttttagt
    27541 acagatttgt aaatactttt tggctctcta taataatcct taaattctag ggttattagc
    27601 tttttcttac ttagagaatt tatactttgt tttgaagcat ttaataaatc caataaatca
    27661 tttatctcta cattttcatt atttttaaga aaacttaata tctctttttg tttacttccc
    27721 acatttattt tattttgctc tatataatcg tcaatcttat cactttctaa actcaaagat
    27781 atataacata ccttcttttc atttttgtga tttttatatt cccaacataa ttttataatt
    27841 ccatttttat tcattgtgta caaagaacta tttaagtttg gtatatattt taacttatca
    27901 accttaattt tacctttact atcaatcact ttctctacta tttctctatt tttatcactt
    27961 aaagttgata ttttttcctt caattcaata tcatttaatc cacttaaact ttcacctaat
    28021 aggactactt tataattatt taacttgtat ccctttggat atatcaaatt tatacattct
    28081 atataagtac acaaatatct atttttcatc caataaacca actctaaatc ttcttttcta
    28141 aatatcggat tttcatctaa tatatcaata atttctttta tttttatttt ttcatctaag
    28201 ctatctgtaa attgaaatac aaaggcttct gttggtttat taccttttcc aaatggtact
    28261 aatattctgt gcccaatttt tattacatca gataaaaata caggaatttg atatgtaaat
    28321 aaattatctg tatatatagt attacttcta actattacct ttgcatattt tttcattata
    28381 aaacctctac ttaaatttaa tatattgttc atattttaat atactttaca agatattaaa
    28441 atctcagaat ttataagcaa tttcaagtta ttaatattaa ctttatttta ataattaact
    28501 attaattata ttttatactt tatgacaaaa taaatgctga aaactacaat ttaactgaca
    28561 tttgcgagtt tttactcgct aaaattaaat tgcagctttc agccacttta gctgtgataa
    28621 aatacacaaa tatcatattt aaaaaaggtc ttatttctca agcaacgctt taactttatc
    28681 taaaataaca ttggctactt cttctttttt catcttagga tactcagtaa tatttccctc
    28741 tacatctatt atttttacaa tattagtatc agctctaaaa ccagcacctt ctttagttaa
    28801 atcatttgcc acaataaaat ctaaattttt cttttttatt tttcctttgg catgttctat
    28861 aaggtcatta gtttcagctg caaatccaat tagtatctta tcttctttta tttttcctat
    28921 ttcataagca atgtcataat ttctacttag ttctattgat aaatcatcat tcttcttttt
    28981 aatttttttg tcagaataag tttttggttt ataatctgca actgctgcac tttgaattac
    29041 tattttattt tcacttaaat ttgatactac tgcttcatac aaatcttgtg ctgactctat
    29101 ttgaacaaat tttttaagat tttgtggaat agaaagattt gtaggacctg atattaatgt
    29161 tacatcagca cctctagcta tagcttctct tgctatagaa tatcccattt taccagaaga
    29221 cctattagtt aaatatctta ctgggtctat actctctaca gttggaccag ctgtaactat
    29281 catactttga ccttttaaat cttgctcttt agtaagatta gtcaaaactt cctctactat
    29341 tatatctgga ttagctagtt tacctgttcc aatatctcca catgcaagcc ttccactttc
    29401 cggttcaata aatttatatc caaagctctt taaagtttgt atattttttt gaacaattgg
    29461 attttcatac atattagtat tcatagctgg tgctataaga acctgtgctt ttgttgccat
    29521 aacagtagtt gtcaacatat cgtcagctat accatttgca atcttgccta taacatttgc
    29581 acttgctggc actattaaga ataaatctgc tttttttgct aatgatatat gttcaacttc
    29641 ccaagttttt ggttcttcaa acatatcttc tactacataa ttttgactca gcgattgaaa
    29701 tgtaagtggt ttaacaaatt ctgttgctga ttttgtcatt ataacatgga tatttgcatt
    29761 tagttttttt aacttactta caacatcaca agccttatat gcagctatac cgccacttat
    29821 gccaattaca acagttttat cttttagcat gaccttactc ctcttctgaa atttgttggt
    29881 gttgctcagc atgatgtcta gcttcttcta tctctatttc ctcttctgtc aataatctat
    29941 atgttatttt tccacttgca acttctttag ttgctacaca tacaggcttt tccttagttt
    30001 tatttgaaac atatggttct tctccatcaa ttaattttct tgctctcttt gaaacagtcc
    30061 ctactaaata gtatctgttg tctatttttt ctagtacctc attgatagat ggttttaaca
    30121 taattataat tcctccttaa atttctctat tataatattt ttatatctgc taactttatt
    30181 cttttcagat gatattatac cttctatttc tttagcagat gtttttacat cttcattaaa
    30241 tataaaataa tcataatcct taatcatttt aatttcttca tatgcacagc taaatctctt
    30301 ctctatttca gcttctgttt ctgtacctct tccaacaatt ctatttttta attcttcaag
    30361 tgatggtggt agtacaaata taaatactcc ctctgggcaa acttctttta tttgttttgc
    30421 tccttgcatt tcaatctcca gtaatacatc ttgtcctttt tctaaacatt ccattattgc
    30481 cgcttttgga gttccataaa aattatcata aatttgagca tactccaaaa actcaccttt
    30541 ttctatcatc tcttcaaact tttccttact tatgaagaaa taatttactc catcaacttc
    30601 tccagttctg ggttttcttg tagtagctga tacagaaagt tttattgtat cattttcttt
    30661 aagtagttct ttgcaaatag taccttttcc tgcacctgat ggtcctgata caactagcaa
    30721 taatcctttt ttcacaaaca tttaagcatt ccccttctaa tattgtattc ttactctata
    30781 ttttgtattt gttctcttat tttttccaat tcgcttttta cttctactac taaattagtt
    30841 atatttatat cagatgactt cgaacctata gtgttagttt ctctattcat ctcttgtatt
    30901 aagaaatcta tttttctacc tatagagtcg tctttaaata tagtattttt aagttgccct
    30961 atatgacttt taaatctaac tatttcttct gttatactgc ttttatctgc atatattgca
    31021 acttcttgtg ctaatctatt ctcatctatt atacttgggt catccagtaa ttcacttatt
    31081 ctcaaattca atttttctct ataatcttct acaacactac ttgaatattt ttcaatctct
    31141 tctatatgat tttttaataa atcacatctc atggcaatat cttcagctaa ttttttacct
    31201 tcttcacttc tcatttctct taatttgatt aatgcatctt caactgcttg attcaacatt
    31261 gaccacaata aatcttcatc ttcttccttt tcttctattt tcacaatatc aggaaacttt
    31321 gcaacattca taacgctaat atcatctata atatcaaatt tattttttat ttcttttagt
    31381 atgtctatat actgagatgc taatccctca tcaaaattta agtttacatc ttctttgctt
    31441 aataagtcaa actttatgta taaatctact cttcctcttt ttacataatt ttttactaaa
    31501 tttctaactt tatcctctaa aaatgaaatt tttcttggaa gacgtatgtt tatatctgaa
    31561 tacttatgat ttatcgtttt acactctact aaaaagtaat aattatcatc cttgtattct
    31621 cctcttccaa aaccagtcat acttatagcc attaattaaa cctccaaaac accttcacat
    31681 atttttactg ctggacctgt catataagag aaatctccta catctatttt aactgttcca
    31741 ccttgagacg ttacatttac actactttct accatattaa gtaaattgca cactattgct
    31801 gatgcagtca taccagtacc acaacctaaa gtatatccac aacctctttc ccaagtataa
    31861 acatgtatat tatacttatc ttctaccttt acaaaattta cattcgtttt ttcaggaaat
    31921 attttattat tttcaatctc ttttccatat ttacaaacat aatctacatc taattcatct
    31981 actataacta ctgtatgagg aacccccatc aacatagaag taactttaag ctccctatct
    32041 aaaaccttta tactttcttg cacaaaacgt tctttatttg taagtacagg aacgtcttta
    32101 gctataaaac ttccttttcc catattgact cttatagagt ttatttctct attggtaaga
    32161 tttattttta tatcttttat accatctaat gtatctactg taaaactttc ttttttaact
    32221 atacaattat cataaacaaa tttacaaaaa catctaaggc catttccaca catagctgct
    32281 cttgaaccat cagaattata atataccatt tctacatctg caacttttga atctttcaca
    32341 actaacaatc catccgctcc tacagaaaat cttctatggc atactttttt tgctaaagca
    32401 gtataatcag atggatttat ctggtcgaat cttccatcta ttgctataaa atcatttcct
    32461 attccatgta gtttccaaaa tttcataagt atcgctcctt cgtaaattaa cgcttaaact
    32521 attataacat aaaaaaattc ccataaactt ataatttatg ggaaaagttt tgtaatgaca
    32581 actataattt gaggatgtga gggagagttg ccattacaat tagggtaatt atattatgtt
    32641 tttaagctag ttctttctat gtttatatat taatatttct atatgaattt tatgtatgtt
    32701 ttatttaaac tgtctatgaa ttttttaatt aaacatctgg taactagcta taatccttct
    32761 agtagatatc tattaacata cacctattaa ttctttaagt gtttttaaaa catagtctaa
    32821 atcttcctta gtaatatcta catgagttac aaatctatat tcaccatttt ccattttatt
    32881 aatttttata tttctttcaa aaaattcttt tattaaaaca tcctctttta taatatcttc
    32941 actcatctta aaaaatacca tacttatatc tggattaaca ttgtttactt caatgccatc
    33001 aattttattt aattcacaag ctaaatattt tgcattttca tggtcttcat tcaatcttaa
    33061 agtcattttt tcaatggcaa ttataccagc tgctgcaact atacctactt gtctcattcc
    33121 cccacccata actttttgct ttcttctagc ttttgctata aattctttac ttccagcaag
    33181 tatagaaccc attggtgcag ctaaaccttt tgacacacag aacataacac tatcacaaca
    33241 ctttgctatt tctttagctt ctacacccaa agttaatgct gcgttaaata atcttgctcc
    33301 atctaaatgt actggtaagt tatgtttctc agctattttt ttaatttctc ttaaattatc
    33361 aagactcaca actgttccac ctgaatatgc attttctaca catataagcc ctgtctgtgg
    33421 ttcatgtatg tcatctcttc taattgcctt ttcaacaagt tcaggattta gagctccact
    33481 ttcgtcttca tctatagttc taagctgaac tccagctatt acagcaggtg ccccaacctc
    33541 atgagtcaca atgtgataac ctttaccaat aattatttct tgccctctta ggcaatgagt
    33601 aaatagagct aattgattcc caaaagttcc acttggaagg aaaatagcat cttcttttcc
    33661 aactttagct gctgctaatt cttcaagttt atttacagta gggtcatctc taaagattga
    33721 atctccaaca ggagcctttg ccatagcttc tctcatttcg tcagtaggca tagtaacagt
    33781 atcacttctt aaatcgataa atctcatttt ttcaaccttc tttcatattt atatactata
    33841 gaaccttaaa attgattttt tctgtaaagt tcatatactt tcatagctta tactctacac
    33901 aataattata ctatatttac tttatattta atctacttta aattattaat tttaaaaata
    33961 aagtttcctc tctaataaat ataaataatc atacaattta aatattaatc tctattgttt
    34021 aaattccccc tagtcacaaa gcgtatatta tgtgtataat taatttactg actcattttg
    34081 gtcaaagaaa ggggtgctat aaatggcatt agatggatta gttatacatt caatagtcga
    34141 tgaattatca tcaaaactta caggtggaaa aatagataag atacatcaac ctgaagatga
    34201 tgaggtaatt tttaatataa gaaataataa agaaaatttt agattggttt taagtgcaag
    34261 tgcctcaaat cctagagttt atcttacaag taattatcaa aaagaaaacc ctctaaaagc
    34321 gcctatgttt tgtatgttac ttagaaaata catacaaggt ggaaatatag tagaaatttc
    34381 tcaaataggt tttgaaagaa taataaaaat aagtgttgaa tctttagatg aattaaaaga
    34441 aaagacagtt aaaaatatta tgattgaaat tatgggaaga catagcaaca taataataac
    34501 tcatggtgaa gaaaataaaa ttatagattc tataaaaaga gtccctttta gcataagtag
    34561 agttcgtcaa gttttacctg gtcatgatta ttcattacca ccagaacaaa ataaactcaa
    34621 ccctcttgat gatatatcta aggatttgtt tattaaaaac ctagaggaat tggaaggacc
    34681 tatctttaag tctatatatt ctaaatttct aggtataagc cctattatag ctaaagaaat
    34741 ttgctacaga gcaggtgtaa atcaaaatgc tatcataaag gatatatctg atgaacaatt
    34801 tgattcttta cacaaagtat tttgtaattt attcaatgat ataaattcta acaaatactc
    34861 tccttgcatt attatagata aaaaggtgga taaggtagtt gatttcagtt gtataaacct
    34921 tacattattt agcgatttat catatataaa taaggatagt atgtctcgta ttctggagga
    34981 tttctataga actaaagata ttaaagatag aattaatcaa cgttcatctg atttaaagaa
    35041 gagtatatct gttaagcttg atagacttta taataaactt aaaaaacaag aagaagaatt
    35101 atcagaatcc gaaaatgcag acatttataa gataaaagga gaattgatta cttcatatat
    35161 ttatatggta gaaaaaggta tggaaagtat tgaagttgct aacttctatg atgaaaattg
    35221 taatgatgta acaatagaat taaataaaaa cttgacacca tctgaaaatg ctcaaaaata
    35281 ctttaaaaag tataacaaaa tgaaacatgc taaagtcgag atatctcatc aaatttcctt
    35341 aaataaggaa gaaatagatt acttagaaaa cataatttta agtattgaaa actgtgaaaa
    35401 tttagctgaa ttacaagata taaaagaaga acttgctaag gttggataca tcaagactca
    35461 aaagaaaaac agtaaaaagg atactatccc atctacaaaa cctcatgaat ttgtttcttc
    35521 tgatggattt aaaatattgg ttggtaaaaa caataagcaa aatgactatc ttacactaag
    35581 acttgctgat aatgatgact tatggatgca tacaaagaat attcctggtt ctcatgtaat
    35641 aataaagtgt gctggtaagg aagtccctga taatactgta tttgaaggag ctatgctagc
    35701 agcattcttt agtaagtcaa aactttcttc tcaggtgcct gtagactata ctaagagaaa
    35761 aaatgtgaaa aaacctagtg gttctaaacc aggtatggtt atttatgaaa ctaacagtac
    35821 tatttatgtt actcctgaag aagaaacagt agccaagctt aaggttaaat ctgaataaat
    35881 tctgcactaa atacaaaata aatgttagac aactacacat tatcattcta aataaaaata
    35941 gtggccttct tataaagtac aataataagg ccactttcta attagcttac ttttaatttt
    36001 ctaacaaaat acttttctgt tttaataact atacttatat tcccaacaaa tattttcagt
    36061 cttcttatat agatgtaatt aaaaagttaa cactaaaagc tattttttaa tttattgatt
    36121 tgttttagta attcattata ttctgattca tacaattctt tttttagatt atctttttct
    36181 atagataaca tagatataac agttgtaagt ctattttcta atactagaag attttctttt
    36241 ttgatttggt tttctctact aggagttttt ttatttctgc taattatata ttcgtcataa
    36301 ttacaatcaa actcccttat tttagcatct tttatctcta taatatagtt acaaatatgt
    36361 gatacaaata ctctatcatg agacactata agcatagttt tatcagtatt tatcagtgcc
    36421 ttttccagtg cttccataga ttttatatct aagtaatttg ttggttcatc aaatactaaa
    36481 aaattattat cttccaatat tattttacat agtgctattt ttaccttttc tccaccacta
    36541 agtactttta cttttttgta gacatcatca cctttaaacc caaataggct taagtttatc
    36601 cttataaaag attcatcaaa tgaactatta actttagtat tgtatagaac actcttttcc
    36661 tcatctaata aactttgatt ttggtcaaaa taacctactt ttaccttgtt atttattttt
    36721 atattgtcat ttttatcagc caatatttct tttatcagag tactttttcc acacccatta
    36781 tcacctaaca atgctatctt tttacctctt tttatcttaa aggaaacatt atctaacagg
    36841 agcttatttt ctaactttag agtcatatct tttacctcta ctagattttt gctaattatc
    36901 tccataccat cttgcatctt aatgttcatt tctttagaaa ctttaggttt attctttacc
    36961 tcaagtttat caattctgct ttttatagcc ttcacatttg agtccaactt tttcttatta
    37021 gtctgaccac ccattttgtg caacctagct tctgaattac ccattctttt tggtgttttt
    37081 cttatgccat cacttaaagc ttttttatat aaaagagctt tttcaagcct ttttttctct
    37141 ttaacatatt cttgatactc aaaatcagct ctttttactt cttcatcttt ttgcattaaa
    37201 taatcagtgt aattgccttt gtaagctttt agttttccat cttctagttc taatatagtt
    37261 gtacataact catccagaaa acgtctatca tgagatatta ataataatgc tccctcatgc
    37321 cttttaaaca tatcttccaa aaccccaatg ctttttttat ctaagtttga agttggctca
    37381 tcagctatta tgatattttt cttattactc agcgcatctg ctatttttaa cttaaccttt
    37441 tcccctcctg ataagtgttc ttcaaattta tctggagcat ttaaaagact cttctcctta
    37501 ctatgactac atgtttcaat attattttca ctctgactta tataagaata gctttctgta
    37561 aggtaagtat atccttcatc aatttctatt tctcccaata ttgcttttaa taatgttgtt
    37621 tttccttgac catttgaacc aactagacct attttgtcat tttctaatat ttcaagttta
    37681 tctatatcca aaattaattt atctgcataa tatttcttta aattctctac ttttactaac
    37741 aacataaaaa atccctcctt aacacactta aatataagga gagattctat aaataggcat
    37801 aacaaataaa cgtatagact ataatctact ctccttaatt taagcattgc aaataaagcc
    37861 accaaaatag gcttttcaaa aattacaata ctttaaatta taaaaaatag attatattct
    37921 cattgtccta gttaccttgc cctttctttc atttattttt aatcattata tcaacattaa
    37981 ttacaaatgt ctatagaaaa ttaactaatt ttagttattc aatatacaac ttattcttta
    38041 aatttattct tcttcaactt taaagtcctg attatttaaa attctgttta atacaattcc
    38101 aacaattgca gctaaactaa gaccagttat ctttactgta cttgttatag ggattccaac
    38161 agaaatacct ttattagata aatatgttgt tccaagacct attaataata taactgctat
    38221 gattaatata tttattttac tttctacaca agaactgtcc cttatagttt ttaatcctac
    38281 ataagttatc atcgaaaata gcattatact aattccaccc ataactgaac ctggtatact
    38341 ttgaagaaat ccaccaaact tgcctacaca tgcaagcaat atagcaaata ttgctgtacg
    38401 tctaagtatt gaagggtcat agtttttagt tatagcaagt actgctgtat tttctccata
    38461 agttgtattg gctggtcctc ctaaacaccc tgcaactata gtcgcaaatc catccccaag
    38521 taatgttcta tttaatccgg gattttctat aaagttttta cctactacag caccattagt
    38581 agtcatatct cctatatgtt ccataaatac agctaataca actggagata taatcacaat
    38641 tgctcctaaa ctgaacttag gaactgtaaa gttaggtatt gcaaataaac ttgcttctgt
    38701 aatagtagtt atatctacta atcttaatat caaacagatt atatacccag aaaaaactgc
    38761 tattaatatt cctaattgtt ttataaatcc tctaccaaac ttattggtta aaattgctat
    38821 tgctaaagtt ataaatgcta ttacaaaatt ggctgaagcc atactaaaag ctgttggcaa
    38881 taaatttaat cctataacta ctatcatagc tcctgtaact tgtgcaggaa aatataattt
    38941 aattttattt acaccaacta tttttactat aatcgacatt ataatataaa ttaatcctgc
    39001 tactattata ccaccttgag catatgctaa atctccacca tacgcctctt tagctgctag
    39061 tataactggt ataaacgcaa aacttgaacc taaaaaggca ggtacttttc cttctgtaca
    39121 aaaatgaaat atcaatgtac caacaccagc acagaaaatt gcaactgacg gattaaaacc
    39181 tgtcaaaatg ggaactaata ctgtcgctcc aaacatggct aatagatgtt gtaaagacat
    39241 tatcgttttt ttcataaaaa cctccaaaat tattatatat aaataaatcc tctgtttaat
    39301 taatatcttt taattaatta tcattcccta gattctattt tttaacctct atatacttat
    39361 tatataattt atcattattt tacattttac gacatcaaaa actcaaataa aggagaataa
    39421 aaactacatt aagactcaca tccataaaat tataatattt attttgtgca atctatgtaa
    39481 gctttatcct cctccctgac tattcaatgc taagctttta tataatactt aaaaataatt
    39541 attctttgat tgtaactgaa tcttctccat caatttctaa taaactgaca gatataattt
    39601 catgtctaga agtaggtaca ttttttccta cataatctgc tcttataggc aactctctat
    39661 gccctctgtc cactagcact gctaattgta ttgatttagg tcttcctata tctataatag
    39721 catctagtga agctctcaca gttcttccag tgtataaaac atcatcaact aaaactactg
    39781 tcttatcatt tacattaaaa tctaaataag ttcctttaac tacaggttca acatgaattt
    39841 tttccaaatc atctctatac aaagttatat caatatctcc tgtatctact tttgttcctt
    39901 ctatttgttc tattttctta gaaattctat ttgcaattgg cacacctcta gtctttattc
    39961 caactaaaac tagattttct acacctttat ttctttcaat tatttcatga cttattctag
    40021 taattgctct tgctattgcc ttttcatcca ttaattgagc tttttctacc atatcataat
    40081 tattgtcaaa gatataatat atctatttga caaatctact cctttctttt aattttttat
    40141 aaatttattt ttatttaaaa tttcaattta aacaacaaaa aaacttctca tcacaagaca
    40201 agaagctctt atataaaaaa tatacagtgc aatttagcaa ttgtcttacg atatctctgt
    40261 atcgtttaaa gataattttt taatctatca aatcaaatta tatatttata tttcaaattt
    40321 ttcaagcttt tttaaacaaa atactttaat ataagatatt ttatttaaat ttcactaaat
    40381 ttatttactt atataaaaac aaaaaatgat ttataagtat tatacaaatg gttattttat
    40441 ctttttaata acttccttaa aatactcagg taattctgaa ttaaattcga tatattcctt
    40501 agtagtagga tgtataaatc ccaacttctt agcgtgcaaa gtttgtcctt ttaatttaaa
    40561 tttagtttct tttggaccat atacataatc acctaaaagt gggtgattta tagacaaagc
    40621 atgaactctt atttgatgag ttctaccagt ttcaagccta gctctcatat gtgtaaactt
    40681 atcaaatctt tctacaactt caaagtgagt gattgcattt tttccatctt ttactacagc
    40741 catttttaat ctatccttag gatttctccc tataggttta tcaacagtta ttttatcttc
    40801 tttaaccaca ccatagcata taaattcata ctccctagtt atagtatgtt cctttaactg
    40861 ttctgctaga aaattatgtg cataattgtt cttagctatc ataagaagac cagaagtatc
    40921 tttgtctatt ctatgaacta taccaggtct aataactcca tttattgatg acaacttatc
    40981 tttacaatga tataaaatcc cgtttactaa cgtattttca tagtttccag gagcaggatg
    41041 gacaaccata tcctgaggtt tatttattat taagacatca tcatcttcat atacaatatc
    41101 tattggaata tcttgaggta aaacttctaa tactttaggt tctggtatct ctataacaat
    41161 attatcttcc tctttaacca gatatctagc tttttctact ttattattaa cctttacttt
    41221 tccatctttt ataatttttt gtatataact tcttgacata tcacctaatt gttctgctag
    41281 ataaatatca agtctaactt cttcttcctc ttcaattact aaaaactctt ttacttcatc
    41341 cattatatca cctactttta ctttcaaaaa ataaaacata tatacataaa aacacagttc
    41401 ctacaactac aaatacatca gctatattga atacatattc ccatataatt ctaaagtcga
    41461 agtaatctac tacaaaacct aatcgtactc tatcaattag atttcccaat gcaccagata
    41521 taattaatat aattcccaac cttccaaata tatgtacttt ccttgtatta agatagtata
    41581 gtccaaatac tgttgcaagt aatgcaacaa ttataaatat ccattgatta ttttgtaata
    41641 atccaaatgc tgcacctcta ttttcgacat aagttaaatg aaatacatta tttattattg
    41701 gtattgttga tacatccacc aaattattca atacccatat tttagacagt tggtctaaac
    41761 ctatgagtag aattattatt aatatatata gcaatgattt ttccccctat aaattattta
    41821 aa
//
LOCUS       DAEMIK010000014        41287 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEMIK010000014 DAEMIK010000000
VERSION     DAEMIK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.014"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            310..1095
                     /locus_tag="KPK87_000644"
     CDS             310..1095
                     /locus_tag="KPK87_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2536836.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(1192..2016)
                     /gene="pdaA"
                     /locus_tag="KPK87_000645"
     CDS             complement(1192..2016)
                     /gene="pdaA"
                     /locus_tag="KPK87_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG2536837.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            2237..5356
                     /locus_tag="KPK87_000646"
     CDS             2237..5356
                     /locus_tag="KPK87_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG2536838.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKGGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            5374..5973
                     /locus_tag="KPK87_000647"
     CDS             5374..5973
                     /locus_tag="KPK87_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG2536839.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(6215..8344)
                     /locus_tag="KPK87_000648"
     CDS             complement(6215..8344)
                     /locus_tag="KPK87_000648"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG2536840.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYDKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            8610..8858
                     /locus_tag="KPK87_000649"
     CDS             8610..8858
                     /locus_tag="KPK87_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536841.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            9024..10415
                     /locus_tag="KPK87_000650"
     CDS             9024..10415
                     /locus_tag="KPK87_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG2536842.1"
                     /translation="MYFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLVQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            10526..11995
                     /locus_tag="KPK87_000651"
     CDS             10526..11995
                     /locus_tag="KPK87_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG2536843.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            12060..12653
                     /locus_tag="KPK87_000652"
     CDS             12060..12653
                     /locus_tag="KPK87_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG2536844.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            12835..14181
                     /locus_tag="KPK87_000653"
     CDS             12835..14181
                     /locus_tag="KPK87_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2536845.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            14363..14734
                     /locus_tag="KPK87_000654"
     CDS             14363..14734
                     /locus_tag="KPK87_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG2536846.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            14727..15416
                     /locus_tag="KPK87_000655"
     CDS             14727..15416
                     /locus_tag="KPK87_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG2536847.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            15936..16802
                     /locus_tag="KPK87_000656"
     CDS             15936..16802
                     /locus_tag="KPK87_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2536848.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            17044..17853
                     /locus_tag="KPK87_000657"
     CDS             17044..17853
                     /locus_tag="KPK87_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536849.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            17997..19061
                     /locus_tag="KPK87_000658"
     CDS             17997..19061
                     /locus_tag="KPK87_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2536850.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            19089..19808
                     /locus_tag="KPK87_000659"
     CDS             19089..19808
                     /locus_tag="KPK87_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2536851.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            19831..20415
                     /locus_tag="KPK87_000660"
     CDS             19831..20415
                     /locus_tag="KPK87_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG2536852.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            20417..21766
                     /gene="pabB"
                     /locus_tag="KPK87_000661"
     CDS             20417..21766
                     /gene="pabB"
                     /locus_tag="KPK87_000661"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG2536853.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            21756..22499
                     /locus_tag="KPK87_000662"
     CDS             21756..22499
                     /locus_tag="KPK87_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG2536854.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            22533..23234
                     /locus_tag="KPK87_000663"
     CDS             22533..23234
                     /locus_tag="KPK87_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536855.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGTKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            23260..23832
                     /gene="folE"
                     /locus_tag="KPK87_000664"
     CDS             23260..23832
                     /gene="folE"
                     /locus_tag="KPK87_000664"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG2536856.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            23896..24687
                     /gene="folP"
                     /locus_tag="KPK87_000665"
     CDS             23896..24687
                     /gene="folP"
                     /locus_tag="KPK87_000665"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG2536857.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            24698..25063
                     /gene="folB"
                     /locus_tag="KPK87_000666"
     CDS             24698..25063
                     /gene="folB"
                     /locus_tag="KPK87_000666"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG2536858.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRARDE"
     gene            25056..25562
                     /gene="folK"
                     /locus_tag="KPK87_000667"
     CDS             25056..25562
                     /gene="folK"
                     /locus_tag="KPK87_000667"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG2536859.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            25543..26328
                     /gene="aroF"
                     /locus_tag="KPK87_000668"
     CDS             25543..26328
                     /gene="aroF"
                     /locus_tag="KPK87_000668"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG2536860.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            26583..28373
                     /locus_tag="KPK87_000669"
     CDS             26583..28373
                     /locus_tag="KPK87_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG2536861.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            28391..29557
                     /gene="rpoD"
                     /locus_tag="KPK87_000670"
     CDS             28391..29557
                     /gene="rpoD"
                     /locus_tag="KPK87_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG2536862.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            29722..30411
                     /locus_tag="KPK87_000671"
     CDS             29722..30411
                     /locus_tag="KPK87_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2536863.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            30425..31522
                     /locus_tag="KPK87_000672"
     CDS             30425..31522
                     /locus_tag="KPK87_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG2536864.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            31585..32157
                     /locus_tag="KPK87_000673"
     CDS             31585..32157
                     /locus_tag="KPK87_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2536865.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(32217..32684)
                     /locus_tag="KPK87_000674"
     CDS             complement(32217..32684)
                     /locus_tag="KPK87_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2536866.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(33070..33546)
                     /locus_tag="KPK87_000675"
     CDS             complement(33070..33546)
                     /locus_tag="KPK87_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2536867.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(33666..34757)
                     /locus_tag="KPK87_000676"
     CDS             complement(33666..34757)
                     /locus_tag="KPK87_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG2536868.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(34750..35139)
                     /locus_tag="KPK87_000677"
     CDS             complement(34750..35139)
                     /locus_tag="KPK87_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2536869.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            35331..35777
                     /locus_tag="KPK87_000678"
     CDS             35331..35777
                     /locus_tag="KPK87_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536870.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            36041..36766
                     /locus_tag="KPK87_000679"
     CDS             36041..36766
                     /locus_tag="KPK87_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2536871.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            36753..37997
                     /locus_tag="KPK87_000680"
     CDS             36753..37997
                     /locus_tag="KPK87_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2536872.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            38077..38754
                     /locus_tag="KPK87_000681"
     CDS             38077..38754
                     /locus_tag="KPK87_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2536873.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLDLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPIGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            38755..>41287
                     /locus_tag="KPK87_000682"
     CDS             38755..>41287
                     /locus_tag="KPK87_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2536874.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNAEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDILFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENI"
BASE COUNT        16881 a         4145 c         7068 g        13193 t
ORIGIN      
        1 agtaaataaa tatataacat gactatagaa atttaacttt aaaataactt atgataattt
       61 agaggaggaa tatatatgaa tatatcaaga aaagctatga aaataataga attagcacaa
      121 aaaattgcaa ataagagagg tatttcagta gaagaagctt ggagtgaagc agtaacagaa
      181 tataaaaata aatacgaaca tattgcatag atatttaaaa atttagaata tttggtagaa
      241 tataatgatt ttaaatgata ctataaatta taaggtatat cattattaac atagaaggag
      301 gatatctgct tgattgtaaa aatagataat actttagaaa aagaattttg gcaatatgta
      361 tcacatgaag aaagtttaaa tttattcata attggatacg ttgagaatta tggatttagc
      421 tctcaatatc aagatatatg gtctcaggta gaagatggaa atataacctc tataatatta
      481 aaaaataagt caacacttat aatttatagt tttaaaaata attttaatat aggagaaatg
      541 aagaatcata taaaagattt agatgtagaa agcataagtg gaaaaaaatg tgtaatagat
      601 agactaataa gtaaatacaa agatttttat gaaaaactag ataataaatt ttgtgtatta
      661 aaagaaataa aagaaataga tttttccaac atgaaggagt ataaaataga aaatgctcag
      721 gaaaaagata ttgatgaaat aggaaaactt ttaaataggt cagactataa agtaagtaaa
      781 aattacatag aagaaagaaa agttcattta aaagagggaa atgtaagagc atattttatt
      841 agaaatgatg atactatgat tagcactgtg tcaactggta tggaaactag ttttttggca
      901 atggtggtct ctgtaagtac agataaaaga tataggggaa aaggacttgc aagttatatg
      961 gtttacaatt tgagtaaaga attgttgtta gaaggaaaag ttccatgtct tttttataat
     1021 aatgatgtag caggtaaaat atatcataat ataggataca aagagataaa tgaatggaca
     1081 attcttttta aatagctata aaaaataaaa aactctatcg agaattaaag tatttataaa
     1141 taactatatt tttactataa ttcatgatag agttttttta aatttatgat actatttttt
     1201 attatttaaa taatcaagtg ttttaagttt ataaccttct ttttcccaat gtgtaagaac
     1261 ctcatctaat atctcagtat ttgttgatga atttggatgc agtaaaacta ttgcaccagg
     1321 atgagttctt gaataaattt tgtttttggc aaattcatga gtaggttgtt tcttttcata
     1381 ccaatctaca taagcaaaac tccaaaatat tgatttatat cctaaatctt gtgtatattt
     1441 taaagattgt tcgctaaatt tacccattgg aggtctaaaa tacttaggca tttcttttcc
     1501 agtaacatct ttgtatgctt tttctacact tgtaatttct tctttgaatt tttcaaaatc
     1561 tgtaatacct gccattgatg gatggctttt tgaatgatta caaactaaat gtccttcttt
     1621 ttccatacgc tttattaact ctggattgct ttttatataa ctttcaacta cgaaaaattg
     1681 agctttagca ttgtgttttt taagcgtatc taataatttt ggagtgtttc cactttcata
     1741 tccagcatca aatgatagat atataacttt ttcattggga tttcctacat aataagctcc
     1801 gtatttttta aaaaaatcag cttcttttat aggtgatggc tgtttaccat cctccctagg
     1861 gttaaaatac caatcatatt catgtgtgtc gagtgttgga ctagaaattt gagtttttgt
     1921 tttatctaat gatttaaaat ttatactagc aattccaaaa agtattagag caaatgctcc
     1981 tatcataatg tattttttta ggctatcttt tttcacaatt tcacctctta tttaaaattt
     2041 aaaatatatt tcttataata tagtttgaat atttatagtt aaaaatattc acatatacaa
     2101 ataataaatg aaagaagttt actgtaagat ataaagttga tattaattat cttaaataaa
     2161 gtgtatataa atgataaaat ataaagagaa atgattgtaa ataagttagg ctggtttaac
     2221 ttgaagggat ataaaaatgg ataatataat aagtgtattg aatgatgttg tttttttaga
     2281 tatagaagtt agtggattag attgtttaaa ttcagaaatt ttagaagttg gagctgtcaa
     2341 ggttaaagac tggaaaatat atacctatga atcattaata aaaaacaaat ttgaggtacc
     2401 tgtagaagta ttctcagtat gtaaaaattt agataaaaat gatttagaaa tagctaatga
     2461 aatagaactg gtggaagata gacttgtaaa ttttgtagaa gatagtttca ttatatgtca
     2521 tgatttaagt ctgaaaaaaa agttttttga gtatcatatg ccaaagttaa aaaacaaatt
     2581 tattgattta attgaattag cagttatttt agaaccatat cataaagatt atagcttaga
     2641 atacttaaaa aatactttaa caaattgtaa ttctaaagtg gaaaatagag ctttatcaga
     2701 tgctattgat ataataaata ttgtaaattg ccttttagta aaatttaata actatgaaaa
     2761 aactactttg gagccattga gttttaaaat aaattcatat cttaaaaaat ttaatcttcc
     2821 aacatgggaa tggagcaaat ttttagaaga ggcaaattat gatttatcaa ataatataaa
     2881 tataaaaaaa gaatataata tatttgactc aaaagaagaa aagaagaagg aaagagaaac
     2941 cttaaaaatt ttaaatgaag aagaaaaaaa ttatgaagaa ttattaaaat acaaaactat
     3001 ttgggagaat aaagaaggat ttacatatga atatagacct ggtcaatatg agcttactaa
     3061 aacaataaga gagttgttta gaaatagcga agatgaagaa aaaattgcat gtatagaggc
     3121 accaactggt ataggtaaaa gtgtaggata tttattgcct gcaatattag aggctagaat
     3181 taataaaaaa agattattaa tttctactga cacaaaggaa ttgcagatac aattaataaa
     3241 taaagatatt ccaaatgtgt tagattcttt gggtctaaat ggaaaagtaa gttacggata
     3301 tataaaggga aaaaataact atatttgtat agatagactg gaagcatata tagatgatta
     3361 tgagtctcaa aaccctacta aaggagaaat tttatcttta atatttttga agagattagt
     3421 tgaaggtggc aagtatggag atatagaaga aataaattat ttagtgtttg acaacttcaa
     3481 ggaaataagt actcacttaa gaaatgtaag ttgtgaccca aatatgtgca gacctaaaaa
     3541 atgcaaaaaa gattgtttat ataaaaacag aatagaagaa ttaaaagaag aacatataac
     3601 agtagtaaat cattcactat tagctaaatg gccgtataag gatgaaaaac ctttagaaaa
     3661 tataatagta gatgaagcac ataatttgac tgaaaaagga tatgatttct tttcaagcat
     3721 tataaattca aagtctttaa ggtatttatt acaagaaatt tatccttacg agtttattca
     3781 aaatagtagc tttatatata aaaaatatag tagaaatatg agaaaaataa aggcttttga
     3841 taaattttat aatgtattaa aaattggaag agaagataaa caaaaaattg ctagaagcat
     3901 aaatctcatt attgaagaga tagattcaat attaaatttt ggtaattgta atgaatataa
     3961 taatgtaagt aattataatt taagatggga actgaatttg caaatagatg aaattgtggg
     4021 aaaactaaaa aagggtggta tagatactga aataagttat agagcctata gtgaaaagat
     4081 aaagttgagt tgtgagaaaa taataaaaaa tttagtttca ataataataa taatttatag
     4141 gaatattgat gatgatagta tagataaaga agcagatata tataaatttg gaaaggcaaa
     4201 gacaagagat ttagaggata taaagattat atttgaaatc tttttagagt atgatgaaaa
     4261 ggatgattat gcaaggatag ttgaaattga taagaattat aatgactttg aatttcgagt
     4321 agtgccatta aaaatagcag atttatttga agaaaatatt ttatctcaat tagaaaaagg
     4381 aatattttta tcagcaactt tgagtttatc tgaaagtatg tcttatttta aaaatacatt
     4441 aggaatagac agagttaaga atgtggaaaa gattatagaa ccaatttttg attataaaaa
     4501 tagagtctct gtagttggat tttctgatat ttgtgagtac agaaattctg aatttccaaa
     4561 tgagatgagt aaaattatat ctaatatatc aaagattaca gaaggtcata cattagctct
     4621 ttttaatagt aaagatagac aagaaaaaac ttatgaaata ttgaaaaaat acttacatag
     4681 ttttaactta gaaatttatg ctgataaaaa gggcattaga caccttaatg atttaaatag
     4741 aaaatgtgta gtacttggct caaaaggatg ttttgaaggc gtagacattc caggggatgg
     4801 tttggtttgt gtaactttag acaagcttcc gaatctaaat ccaaaagacc ctttgtattt
     4861 tactataatg aagaagtatg gtatagacta ctatactata aattatcctc aaatgaccat
     4921 aaaggtaaag caagctatgg ggagaatttt aagaagcaaa tatgactatg gatgttttgt
     4981 gatttttgat gtaggaacta atataagtgt tttaaagaga ttagagaaag atttacacga
     5041 ttgtaaaatt tcaaaggtta atagcaatga gttttatact tatataaggc gacatttaaa
     5101 taaatctaga agtttaatac taaaatctgt tatatttgat actataaaag cgttgaatgt
     5161 tgatgctaaa atggataaca atgatgttga taaggatatt ataaaaaaag atataaatga
     5221 aaatataaga caaagagctg taaaaggaga agtgtaccat attgatatta taaaaaaaga
     5281 tatgaaagta aaatattttg atagaaacta tcttataaat ttagatatat ttatgagaga
     5341 agaagataaa aattaagagt gggagtgata tatttggcaa aacaaaagtt ttatgctgtg
     5401 aaaaaaggta aaaatatagg tgtatataat acatgggatg agtgcaaaaa acaagtaaat
     5461 ggattctcag gagctgaata taagagtttt tctacttttc aagaagctaa agaatatatt
     5521 gatgggtcag aaaaattgag ttttcaagaa gataaggagt ttatagaagc atatgtagat
     5581 ggaagttatg aacattcagt aaaaatgtat ggttcgggtg ttgtgatttt aaaaaataat
     5641 gaagttataa aaacttatag tgaaaaagga aaagagaaaa ctttagtaag tatgagaaat
     5701 gtagctggag agatagaggc ttctaaaatt gcaatgcagt actgtattga caataatgtt
     5761 caaaacttga ttttatactt tgactatgaa gggattgaaa agtggtgtac tggagtttgg
     5821 aaaacaaata aagaaggaac tatagcatat aaaaatttct atgacagtat taagaataag
     5881 cttaatgtaa aatttacaaa ggttaaagct cattctggaa ataagtataa tgaggaagct
     5941 gataaattag ctaaaaaagc tattggtgta taataaaaaa atatttatac ataattacat
     6001 ggtataagag tatatctaaa ttcctacttt ataattaaac aacttgtaat taaacaactt
     6061 ataattaaat aaattttgat taaaaaagca gattgtatta tgaaaaaata agtatctaat
     6121 ttataaacaa ttaataatag tattaattag atacaactta tttaatataa taaaatctgc
     6181 tttttatata actataaaat tttcctacat tctattagaa ttgcccataa ataccttcaa
     6241 gttcttcttt taatacttca gggtcattta taccaataga catggttcta gccagtatat
     6301 ttgcttggtc ataagttgat gtccatgaga atacaccacc tagacaattt ctaagaacat
     6361 attgagtttt taagaatatt gataaagcat catcatatga cattgcaaaa tctccatcct
     6421 taactaaata tggaacttga gcagtattat caaatctata ttcatatcca tttttattta
     6481 tataatccct tctaagctca tcataagttc ttgttatagt agcacctaat cttccataaa
     6541 atggtatacc aagaaggatt ttttcagaag gcattccagc attctcaaga tttcttacca
     6601 ttgcatcaac actatatcct ggcaaagata agtctgaatc aaaaagattt gcttgatgtt
     6661 ttctaccatt tgggcctgtt tcacctgctg taaaatcata actcataaga ttaaaataat
     6721 ctattatagg agctatttta tctatttcag cacttgaatt tatatatcct ctatctcctg
     6781 ttccagctac actaagccat ttatcatctc ctataacatc tcttatggca gttagtaaga
     6841 gtgtaaagtt ttctctatct tgaggtcttg atgttattcc agatgcacta cttccaggat
     6901 attcccagtc tatatctatt ccatctaaag catattcatt tatcatctga ttgacctgtc
     6961 ttgcaaaatt atatctagat gtaggtgtta atgctgcatc agagaatcct tcagctcccc
     7021 aacctccaat agctacaatt acttttaagc taggtttttc acctttaaga gcaagtaatt
     7081 gatttaaaaa tctaggataa ggaataaaca aatttccaga ctggtctatc tcagcaaaag
     7141 caaatataac tgcatctaat agttgagcat ctacattagt tggatttcca agaacatatt
     7201 ctgctacaat tggatttata tctggacagt ttgcattttc aactattgga ttgtatgggt
     7261 ctgttatttg tggtctaact aatttttttg aatcttttaa agttttcata gctatttttt
     7321 tagtattttt ttcgctagta taatcgtagt ttttataatt atgtttatag aaatcttcat
     7381 aactactatc ttctttatca tagctagaat ttattttgtc gcaagattca tctttatcat
     7441 agttaaaatt catttcatca caagacttat ctttaaactt atcttgtttg gagttgtgaa
     7501 ctttgttagt atttttagta tgttttttct catggttgca atcatcttta atatcagtct
     7561 tgtgttcttg tttacaatca agagctttgt tcaggtagtc atgatgttca tgttcacagt
     7621 tacattcatt ttcaatgttt ttagtatgtt cttttttcca gctacaagta ttatcaattt
     7681 tcttgctcac ttcttcatca ttataattat ctggtacaaa acataagtac cagtccatac
     7741 atgaatattt cttattacaa ctgttaactc tattttttaa ttccttatag tttttaggat
     7801 aaggtaaaat cactggcatt ccaggaaacc agtttgcagg agtaactata ttatctctat
     7861 cactagtctg aagtgcatct actattctaa gtatttctgg aatatttctt cctgtagtta
     7921 gtggataata aagaatcgtt cttagaattt gattattatc tataataaat acagagcgaa
     7981 cagttgatgt atcactcatt ggttttgata tcatgccgta taacttggca attctcatat
     8041 ctctatcttc tataatagga aatggaattt ctacacctgt aagtaaagaa atattataca
     8101 tccaagctaa atgtgaactg ttactatcaa cacttagacc aattagttct gtatttcttt
     8161 ttttaaattc gtcataatat ttagcaaaac ataaaaattc tgtagtacaa actggtgtaa
     8221 aatcaccagg atgtgaaaat aaaacaatcc aattaccctt atagtcagag agtctaatag
     8281 gaccatttgt tgtattggct ttaaaatcag gagcctttga ccctaaactt ggcaaatttg
     8341 gcatgtaaat cacccctcaa taaattatca tttattttat tctattaatt aatacatatt
     8401 agtgttacta ttttattaaa agttaagtaa tccttatgtc aaaaaaagat atttattaaa
     8461 aagatttaat attttcctaa tataaatgac aatagtaatt gctatttttt tactaatttg
     8521 gtatagtaat ggataagggg tagtgtcaat tttagtgatt atacgaggtg agtggattga
     8581 aaaaaattct aagaatttta gttatctcca tgatgatatg ttttgcaatg acttttgcat
     8641 tagtgttaat aaattcagtt atgggagtac atataggatt ggattattac tttttgatgg
     8701 gaagtgtgct tacagtttgt ttaacaattg gaattgtttt atgcaatgct agaaaaatat
     8761 ttgatagcaa agctatacac aagacaagaa cgaaaactgt cagtactaat aaaaaacaaa
     8821 agagtcattc tagggatata aaaagaaaga tatcataatt tatgtaaagg aaatatttat
     8881 gtataaaata atgattgata ggtaaaataa aaacacgatt taacttgtgt ttttattttt
     8941 tttcaagaat tatgatataa taaccaataa gaaatttagt ttaccataaa atataaagat
     9001 tattaaatgg agagggaact ataatgtatt ttagagattt atttgagaag gctgtagatt
     9061 ttattgatga aaaaaggaaa tctatagttg cgatttcatt atcaacatta ggtgtaattg
     9121 ctttgataat agtatttttc ttaagtagta acgagtttag tgtaggaaat gaagctaatg
     9181 agttgttaaa gattatagag aaaagacagt attctatagc tgttgattac tacacaagta
     9241 tagaaaaaaa gttttctgat tcaaaaatgg aaaggtttaa taagtctgtt tctaaaaaaa
     9301 taaataaatt gctattaaat agtggtgata aatatttaga tggagacata agtcaagaaa
     9361 gttttattgg attaataaat accgtgaaag aattaaacaa aataagtata gatgtggatg
     9421 acttattagt tcaagctgat agagttagag aaatgtataa agaagaaaat actacttatg
     9481 atattgccat taactacata agtactgttt caatattaaa tggtatgggt agtaacctag
     9541 atgtatacaa acagaatata gaaacaatca aagagtctag agatgtttat gactcagcag
     9601 taaaagacca aaaagcatat aagtattatg aagctattga aaagtataat aaagtattaa
     9661 aagaagatga aaagtattac agtatggctc aaaatgggaa agaacaatgt attgaagaaa
     9721 tgtatgatta ctatataagt agagctgaag aggcaaatac ttctggagat tatgaaagag
     9781 cattacaata tattgagtat ttaaaagacg attattcaga tgatgaaaag gtacagagtt
     9841 tagaaaaaaa atataaaaag aatttatcat tgtatacttt aacatctgat gacattataa
     9901 atgtaatagc taaaaaaagt ggcaaagaca aggcaaatct atctataaat tcacttccac
     9961 aaatgataaa aaataataaa tactattatg ctgaagtata tgagtatgat aaattaataa
    10021 atgaagtttt ggtaagtgca aaaactaaag atttatattc atataaagat ggtaaaaaag
    10081 attacaaagt agattatggt gatggatact ttagaatatt agaagatgga agttatcaat
    10141 ttggtattac aaaagataaa gctaagtttg tgttaactaa tactctcgat gaaaaagaaa
    10201 ataagtataa aaagattgag atattagata tagagaaagc agatagatat gtaaaaagta
    10261 aaaagagttt agaagaattg tttggaaaat ataaaaatat ttattattat gctgtagtaa
    10321 ataaaggctt atttagaggc aaagaagttt atgctattaa tatttacaat gaaaaaatat
    10381 atgccatatc agaaaatgga ctaaatgaat attaataata ttaaattaat atataattaa
    10441 aaagtttttg gggttttaag gcgcattatg tagtaatata ataataggga tatataaata
    10501 attttattgt aaggagaagt atgggatgag taatgtgaac aaaaagctag ttatagctat
    10561 tggagctgta gcattagtag taataatttt tataggcata atgtgcatgc aatttaaagg
    10621 ggagaaaata gcaaaaaaca catatgttaa tggagtaaat attggaaaat taactaaatc
    10681 acaagcaaaa caggaattag ctaaaaaata caaattagag aatgttgatt ttaattacaa
    10741 tgataaaagt tggaaagtaa agagcaaaga tttaaattta tctactgacc tggataaaac
    10801 agtagaaaac gcatataatt taaatagaaa gagtgctttc tttggcaatc tatcaaaaac
    10861 tatttcagca aattttggca aaaaaagcaa cttagtagtt gtgataaatt atgataaaaa
    10921 taaattaaaa gcagaaatgg aaaagatagc taaagaaata gatgtagatg taaaagatgc
    10981 tacacttgat ataagtggtg aaaaagtcaa ggttatacca gattctgatg gtcttaaaat
    11041 ggatatttct aagagtatgg agaattttga taatcaaact aaaaaaggta actataaaaa
    11101 tgagttagta gtaaaagcta ctccagcaaa agtaaaaaaa gagcaacttg ctaatataga
    11161 tacaaattta ggtacgtatt caacaacatt taaaacttct caaataaata gaagtataaa
    11221 cataaaatta gcaacagata atataagtaa tgtattactt atgccaggag aaactttctc
    11281 atttaataaa catacaggga aaagaagtaa ggaaaatgga tataaaagtg cacctgtaat
    11341 aatggaaggt gagatggaag aagattatgg tggaggagtt tgtcaagttt catctacgtt
    11401 atacaattct gtgttatacg caggtttaga aattgttaat gtaaaaaatc atactatacc
    11461 atcaagttat gttccaaaag gaagagatgc aactgttgca gatagtggaa tagatttctt
    11521 atttaaaaat aatctaaagc atccagttta cataaaaaat tacgtttcag gaaatcaaat
    11581 tgtttgcaat atatatggaa gtgcagaaga taaacaaaat ataacaatat caacaaaatt
    11641 agatggcgtt tcacaaacta caatgaaaag agttaatgac cctacaatgc ctaaaggtaa
    11701 agaaaaagta gacaaaagtg gaagaaatgc atattctgta tcaacatata gaacatttaa
    11761 tgatgcaaat ggtaaaaaaa ttaaaactga aaaaatagct aattcatatt atcctaagaa
    11821 ggaaggtatt atattagttg ggactatgga accaaaacca gaggaaaaac caaatacaga
    11881 cgaaaataag aataatcaaa atacaaacaa tcaaaatcca aataatcaac aaaaaccaga
    11941 aacaccacca actgataata agcctaatga gacacaacca caaccgcaag cttaaagttc
    12001 tcaagaatag gatttatgta gagtaataaa aaatataaat ctaaatagga gggattactt
    12061 tgaatttaaa taatataacg aattacgttc aaagtatttg cgagaatatt tctaatgtct
    12121 tggatgtaga tgttacttta gttacaaaag atttaactag gatagcaggt acaggaatat
    12181 tcagaggtaa aataggagag acaatatcag agaagtcagt gtactctctt gttttaaaag
    12241 atggaaaatc ctatgttata aataaagaaa tagaagagaa ctgtaataaa tgtgcaaata
    12301 gagaaaattg taaagagtta gctgatatat gcactcccgt taaaatggga gagcatatac
    12361 ttggaatctt gggtatagct gcgtttaatg aagaacaaaa agaaaagata ctttcaaaag
    12421 ataaggagtt aactgctttt gtaaggagta tgtctgattt aatatctttt aaactagatg
    12481 aactttataa acaagaagtt aagactatag atgaccttga gagggaagct atagaaaatg
    12541 ccataaagaa atatggaagt aatacagaag gtatgaaaaa agtagctgag gcattaaata
    12601 taggtatagc tacactctat agaaaagtaa aaaaattcaa cattaaaagt taatagtatt
    12661 gaaaaagagg taaccttttt atgaaaagaa gaggattacc ttttttatta tgagaatggt
    12721 ggaaaaatat gtatagtttt gacttgacat attaaatatg ttatcttaaa tatatatttt
    12781 agagtggagg tgagtattat tttagttaaa gatataaaac aagacataga aagcatgcct
    12841 agaaaaattt tagatatatc tttgatagaa gataataaaa gtgatttgac tttttttaat
    12901 atagaagaag aagacggtga tgaaacatta aaagcattaa aacagagtta tgtgactaca
    12961 aattataaag aagaaactct attaaacttg caaggagctt taaattcaaa gtcagacaca
    13021 acgtctaagg agttaaaatt agaatcatat aaaagatata acaatgcatt ggatttagca
    13081 tataaaaatt atataactag tgccattaat atggttaata aagctctgga gataaatcct
    13141 aaagacattg atatactaaa tcttagaggt cttttaaaat tattaaaatg tgattttgct
    13201 aaatcatttg agagttttta tactgcttta tgttatgaaa ataatcatat atctagaaaa
    13261 tatgtaaatc taatttcatc tgaagatttt aaaacttttt taggaagata caatcattct
    13321 ataagattta taaatgaaga attaaattat gagtcaatac atattttaga gaacataatt
    13381 gaagaagaac ctgaactaat agagccatat gtaattttat ctttgttata tgataaacta
    13441 ggtaatgtaa aaaagagaga aggatattta gacaaactta aagagctaga taaagataat
    13501 cctgtatttg aaaaaaatga agaagaaaaa gaagatacaa gtaaaaagga agaaataaaa
    13561 aaagtaaaaa gaaagaaaag tattttgcca tatattatag ttggctgtgt tttaataggt
    13621 atggggatat atttgattca aagcaaaaag agaatagaaa acttgaataa ccaaataagt
    13681 agtaaagagg aaaagttaag tgaaactgat aaaaagttag gagaaacgag taaaaaatta
    13741 gaaaaaacga ataaggaatt aaatgaggtt aaaaataaag aaccagaaag agagataact
    13801 gtagctgatg aggaagattt atattatcaa gctctaaatt taaagaaaaa caaagaatat
    13861 gaaaaagcca tagataattt taaaagtgta gtttcttctg gtaaaactaa aaaatatatt
    13921 tctgagtcaa tatatcaatt agcaataaca aataaattat taggaaataa ggatgaagct
    13981 ataaaatatt acaaaaaata tataaatact tatactaaaa atgaccagta ttatgatgat
    14041 tcatattacg agttgggtat gctttattat gataatggcg atttaaaaaa tgcacaacag
    14101 actttttata gtttgagaag tgaagttcca gacagtatgt ataacaattc aaaaataaaa
    14161 gaaattttaa gtgaaaaata ggtttatata gttaagtttt ttgaaacaac caagtatgta
    14221 ttttattaaa ttaaaataaa aataaattta aaaagagatt gtgtcaaatt ataactttct
    14281 attttaaaga aagatatttg cttgttgata aaactacaaa tatatgattt gaagcaatct
    14341 ctttaatttt tggaggtgga aaatgaaagt tttcaatcaa ttagtaataa ttttatctat
    14401 ctgggtaata ggagagtata taagttcctt tatacaagga attatactta ttcctgggag
    14461 tatagttggt atgctattat tatttctact actacaattt aaagttttag acttgagtag
    14521 tatagaaaat gtaagtggat ttttcctcga taatatggct atatttttta ttccagcagg
    14581 tgtatcactt ataaagtcat tagacctaat aagggaaaat gtattagtgc ttttattagt
    14641 catatgttta agtacactaa tagtaatgta tactactgga attattgtgg agaaaatgat
    14701 aaaaaagaag gaagaaggac aaaaaaatgt ataatgtatt acagacacca gtatttggta
    14761 taatagtaac aatagttttt ttcaatttag gaagatacat tcaacgaaaa acaagtaatc
    14821 ctatatgtaa ccctttatta attgcaatcg taggaataat attgttttta agcatatcta
    14881 aaatccctta tgagaattac aagataggag ctgatagtct aaatttcttt ttaggtcctg
    14941 tcacagtagt acttgcagtt ccactttata aacaatttga attatttaaa aagcatatgt
    15001 ttgagattat tgtaggaata ggttgtggta tagtgatttc ttttgtatct gtattgataa
    15061 ttggcaaaat agctaattca gatgtaagta taataaattc actaattcca aaatctataa
    15121 caacaccaat gggaatatct ttagcaaatt ctttaaatgg tatagaatca ataacagttg
    15181 tagccataat ttttacaggt atttttggtg gtatggttgc ttctacagta ttcaaactgg
    15241 ggaatataaa tcatccagtt gctaaaggaa ttgctcttgg aacatctgca catgctttag
    15301 gaactacaaa ggcttttgaa cttggagaaa tagaaggagc tatgagtggt gtttctattg
    15361 gagtctctgg tacaattaca gtaatactaa ttccaattat aatgaatttt atataaaaaa
    15421 taaaattaag acgagtacct tgaatactta aaacaaaata aaataaaaaa aatttaaaat
    15481 aaaaactgaa cgaaaaaaat gcaaataatg aaataaaaaa atcatgtact gaaattataa
    15541 aaagaaaaat ttcatttagg aattaattat aaagagacaa ttactaaact tgaatttgct
    15601 aaaataaaat aaattactgc gaaaatagat atttagaaaa aaatactata aatttactat
    15661 attgcaagtt aaataataag cagtatcata ttatgctaga aaaagtttca tttaaggcat
    15721 aaatgagatg atttgaacgt ttttaaagat tagaatataa aagattgcac tagacatata
    15781 tgaaaaagat aatataatag atgatgtaat agatgacagt tcaagaaatt ataaaaaaaa
    15841 tttatttgaa atattatcta ttacatcagg aataatatat gtaagagtaa gcttttaaaa
    15901 ttctaaagat ttagaaagat tgaagggaga aagaaatgag tgaagttaag aataccctac
    15961 aaaaacatcc agttttaaga tattcaacaa tgctatttgg gatgttgatg acatctatag
    16021 gaataaatgg gttcttaaga ccagcacatc tacttagtgg aggtgctaca ggtatagcaa
    16081 catctataaa ctatttgaca aatataaatg taggtttatt aacattttta atcaatattc
    16141 caatatttat cttagggttc atataccttg aaaaagagtt ttgtatttca agtttggtaa
    16201 atatgattgt attttcatta ttattaggag caactcagga aataagtaat attataccta
    16261 tacatgatat attgcttcaa agtgtatatg gtggtatatt atctggttta ggagtcggag
    16321 ttgtatttag aactaggtct tctcaaggtg gtacagatat aatagctgca atactaaaaa
    16381 ttaagaagaa tatagaaatg aaagacactg ctttagcaat aaatggcttg atagtactta
    16441 caggaagttt tttatttggg ttggatttag cattatacac tcttataggt ctatttctaa
    16501 atgcgtattc aatgagtttt attaaagatg caatgaatta ccaaaaatct gttatggtaa
    16561 tgtcaaatga agtcgattta atagcagaag atataatgaa gagtttagtt agaggtgtaa
    16621 cttttttaga tgcagaaggt gcatacactc atcaaaaaaa gaagattata tatacaatag
    16681 tttcatcaaa tgagatacca aagataaaag atatagcttt gaagtatgat aaaaaggcct
    16741 ttatatctgt aaatgatgtt acagaagtaa aaggaagagg gtttaaagca aaagatttat
    16801 agaaaatttt acaagctggg agtttataat gtaaatttgt atattatatc tctcagctta
    16861 tttaaattta gtatacgaaa ataaaggttt tattaatgat ttgtgtatca ctttaaaaga
    16921 tatcatttta aaattaaaat tgaaaaaatt acttaatatg tagaaaacat gaccactatt
    16981 aggatataat aaatttaata gatttagtaa aatatctagg aataacgttg gaggaatggc
    17041 attatgaatt tatctaaaga atatgttaat ttgtatgata attatttgaa atatataaat
    17101 gaagtaaaca aagagattag aagtataaag aacatgaaga aacgtgtttt aaaaaggggc
    17161 gattttgctc cggaaaatga agtattagaa ttggaaggaa aaacaatacg cgatatagat
    17221 gatgtcgata caaaaaaacc aaaaaataaa atctataaat tttcaaatgg ggatgtatat
    17281 gtagggaaat tcttagaggg aaagatggag gggaaaggct catatacttt ttttgtagat
    17341 gaaggaacag ttatggagta cataggagaa tttaaagaag ataaaaaaaa tggaaaagga
    17401 aactttatat tttcaaatgg aaatgaatat ataggttatt ttgaggaaga tatgatgagc
    17461 ggtattggaa atatgttata tagtagtaaa gatgaatata ttggaacttg gaaaaacgga
    17521 aaaaaagatg gaaaaggtat ttataagtgg agtgatggat gtatatatgc aggtgaattt
    17581 aaaggtggaa agatggaagg gtacggtatt tgctataata gtaaagggga agtactttat
    17641 gaaggagaat ggaagaataa tttgattcat ggtaagggaa cttatatatg ggaaaaaggt
    17701 aaaaagtata taggtgaatt tatgcatggt aaaaaacatg ggcaaggaac tttttatcta
    17761 aataatgaat tggtttatga agggacttgg aaatttgata agccatctat atttgataga
    17821 acattagatg aaatattttc cttgagactg tagctttatc taattagtag ataaagcttt
    17881 tttctaatac ttactgtaaa taggtatgaa tgatattgat tagtatacac tatcaatatt
    17941 aacttaataa atgcaatatt aaaaaatttt gattgatttt aagaaggaga gatatattga
    18001 agagattaat tgctttagtg ttagtagtac ttattgtttt attttatagt cctgaattat
    18061 taaaagaagg atatgattta aaagaaaagt tttttcaaag tgaaatatgg aatgaatttt
    18121 tagacaaaat aaatataaat gataattcta agaaagataa aaaagaaaaa agtcaaaatt
    18181 tacaatcaaa taatactgaa ggggagaact taggagagtc agatataaag aattttgaaa
    18241 aaataagctt aggagataat ttaagctatg ttttatcaag tatcggcaaa cctggtagaa
    18301 ttgatattag tgaatatggt tttgattggt atgtatataa tcaatatgga aaagaatttg
    18361 caatggttgg acttgaaaat gatgaagtag tagcacttta tagtaattct attaattcat
    18421 gtgaaaatca agatataaaa ctaaatcaag atagacaaac agtaagaact aagattacac
    18481 cattaaaata taagagaaaa ggaaatacca gatatataat aaactcagaa aatcaatatg
    18541 atataataag taaggaaggt aagtatatta caattttcta cgatattcat gaagaaaata
    18601 gagtttgttc atacttaata attgataaaa gcactgaaga tgagtttaaa aatttatatc
    18661 cagatgatag cgaagaactt aaaaaatgtt ttgaacttga agttatagac cttgtaaata
    18721 gtgttagaaa tcaaagaggg ttaaactcat tgagatacag tgaacaggca actttgagtt
    18781 caagaaaaca tagtgaagat atgagagata ataatttttt tgaccatgta aataagaaga
    18841 atgaaactcc atttgacaga atgaaaagag aaggtatagt atatacttca gctggagaaa
    18901 acatagcagc aggacagatt aatgcaatat atgcgcatga agcttggatg aactctgaag
    18961 gacatagaaa aaatattttg gggaactata ataatattgg agttggagta atatttggag
    19021 gaagctataa aacatattac actcaaaatt tttataaata actaaaatat ttgaaaggaa
    19081 gttaaaaaat gacatcatgg aaaaaaaaga cagtatataa gtgcttaata gcagtagtct
    19141 tgttttgtgg aatagtgtta atatctaatt ttagtaaagt ttctgctttg atgatggata
    19201 ctaatggaaa tgttttgatt aaacatggtt caagggaaaa aaagctaata gcaattacct
    19261 ttgatgatgg tcctcatcct aaagaaacta gtcaagtttt agatgtatta aagaaataca
    19321 atgtaaaagc tacttttttt atagctggaa aacatgcaaa atggtataaa gaaccattag
    19381 taagagcaag taaagaaggt cacgagatag gaaatcatac tttcaatcat cctgatataa
    19441 gtaatttaag ttctagtcag atagaagagg agattgttaa gtgtgaagac attttgaaag
    19501 aagtaactgg aaaaaaaccg acattattta gaccaccatt tggaagttat agagaaaaag
    19561 aattaataga aattgcaaaa aagcatgact ataaagttgt tttatggaca ggtgtagatg
    19621 tgaaagattg gaaaaatcca ggagctaata gcattgcaga taaaataatt aataaagtac
    19681 aaaatggaga tataatactt ttgcatgatt atgcaactaa tgatacagtt gaagctttag
    19741 atatgtttat tccaaaaatg attgaaaaag gttttaaatt tgtaacagtt tctgagttga
    19801 taaaataatt tttaggggga gatttacagt atgattctta tgatagacaa ttatgattct
    19861 tttgtataca acctagtcca atatatagaa gaattaggag aaacagttgt agtaaaaaga
    19921 aataatgaaa tcaaaataag tgacattgaa gaactaaatc cagaggtaat tgtgttatct
    19981 ccaggaccgt gttctccaaa agaagctggt atttgcatag atattgttga gcattttaag
    20041 gggaaaaaac ctatactagg aatttgttta ggtcaccaaa ctattggaca tgtttttgga
    20101 ggagatatta taaaagcaca acagcctgtg catggaaaag tatatagtat caaccatact
    20161 aataaaggag tttttagggg acttaaaaat cctttaaatg tcactaggta tcattcacta
    20221 atagttgact ccaatacagt tccaaaagaa ttagaaatta cagcaattac tgataaaggg
    20281 gaaataatgg ggattaggca taagaaatat ttaatagaag gagtacagtt tcatccagaa
    20341 gctattttgt cagagtatgg tcatgaaatg cttaaaaatt ttataacaga agctagagaa
    20401 agggtacata tgtaatatga taagagagat aaatactaaa ttaaattctt ttgagatatt
    20461 tactattttt agaaatgaac atgacagttt tatattagac agtgctatgg ataaagaaaa
    20521 attgggaaga tattctttta taagtagcca accatttaag gtattaaagt ataaagatac
    20581 tgatgaaaat cctttagaag ttttaaaaga agaactacat aagtataggg ttgtaaatga
    20641 tactaatctt ccatttgtag gaggtgctgt gggatattta tcttatgatt taggtaatta
    20701 catagaaaat ttacctagaa ctgctgtaga tgatattgag atgcctgata tgtattttgg
    20761 attttataat catgtaatag ttatagacca tcttgtgcaa aaaacttata tagctactcc
    20821 aaacatagat atcgaattag aagaaaagat aatagatgat atagaacaaa gaatattaaa
    20881 agaagagaaa aaaggtatag atagcatatg ttatgaagaa aaagaagtaa cacctataag
    20941 acttaagtct aattttacaa aggaagaatt taaaaatgca gttcaaagtg ttagagagta
    21001 cataagacaa ggagatatat atcaagccaa tttaacacaa aggttcagtg gagaaacaga
    21061 acttacaagc tttgaattgt atagggactt aagaagattt agcccagcac catttggggc
    21121 attcttaaac tttgaagatg cacatatatt atcaaattct ccagaaagat ttataagatg
    21181 tgttaataag agaatagaaa ctagaccaat aaaaggtact cgtccaagag ggaaagataa
    21241 agaagaagat ttaagacttc aacaagaact tagaaatagt gaaaaagata gagctgaact
    21301 acttatgata gtagatttag aaagaaatga tattgggaga atatctaaaa ctggaagtgt
    21361 taaagttcca gagctatttg taattgaacc atatgcaaat gtaaatcact tagtttccac
    21421 agttgtaggt gagttaaaag atgataaaga tgctactgat gtaataaaag caacttttcc
    21481 aggggggtct ataacaggag cacctaagat tagagcaatg gaaattatag acgaattaga
    21541 gccaacgcaa agaaatgtat atactggttc aataggttat ataggtttta atggtgatat
    21601 ggattttaat atagcaatta gaacaataat caaaaatgac aaaaaagtct actttcaagt
    21661 tggaggagga atgacttggg attctgaccc agatgaagaa tatcaagaaa cacttgataa
    21721 agctaaatcc ataatgaagg ctttgagggg atattatgaa gaataatgta ggttttgata
    21781 gtagtttaag taaatttgga attggtttgt ttgaaactat tagagttaaa aatggaatag
    21841 cagtagattt agatatccat atagaaagaa tgctaagctc aataaattgt ttggatttag
    21901 atataaacta taaaaaagat ttcttgataa atgaaattgt tacatatata aaaaaagaaa
    21961 atgtcattaa taaagcactt agaattactg tttttgatga aggttataat atatctatta
    22021 gggatattcc atataatcaa gagacgtatg agaaggggtt taaattagct atatctccaa
    22081 tagttagggg gaatagttta attcatagac ataagactac taattatttt gaaaacatat
    22141 ataccaaaaa tatcgcaaat aagactggtt ataatgatgg tatattttta aattcagaag
    22201 gtgtaatatt agaatgttct atgagcaata ttttctttat aaaaggagaa agagtgtaca
    22261 ctcctagtga tagattaccg atattaaatg gaataataaa aaaaaggatt atagagattt
    22321 gtgacgaatt acatatagag ttgatagaaa atgaaattaa tatatcagaa attagtagtt
    22381 ttgattttgt ttttgtcaca aatagtctta tgggcgctat aaaggttaca gaaattgaca
    22441 aaataaaatt taataaagaa aatgttgttt ttgataaaat agtgaagtta ttagaataaa
    22501 ttaaaatatt aacaatttgt tggagacaaa agatgatagg ttgtatttat gaagtataca
    22561 atgaagagat aactaaaata atagaaaacc aaaacacttt ggctgtatat ttagtgggtt
    22621 ctagtaaaga cgtagatttt actctttatg atgtagagat aaacgatatt gatgtgttcg
    22681 tttttgtaaa agaaggggaa aaacaagaga gaatactgtt taaaaaaaag ggaatagaat
    22741 ttgatgtaaa ttacttttca aaagatggtg taaaaaaatt attatcaaat agggaatatt
    22801 tctttgtgaa agaaatgaaa gatgctaaag tactgtttga tagggagaat atatcacata
    22861 ttataaaaga tataagtaga gatatttact tagaaggacc tagtaaaatg tcattagagg
    22921 aaaagtcttt tataaagcaa gatattggaa caaaaatatc gaatttaaaa aataaagaaa
    22981 aatttgatgt ttttgaatat cattttttga cgaatttata tttaaaagat ataataattg
    23041 ggtactttaa tataaatgat aaatgggtac caaaagacaa aaaattgcta aaagttttaa
    23101 aaaaagaaaa taatgagctt tttgaactag tgtcaaaagt aagtgaaaat tatgactatc
    23161 aaagactttt agatgtttat aattatattt ttaaagaaat tgagacaaaa gaggttataa
    23221 aactaatctt ctagtcacaa atcaatgggg ggagtttaaa tgaataaagt tgataaagaa
    23281 aagatacaac atgcagtaag agaaatacta gaggcaattg gggaagaccc agatagagaa
    23341 ggtctaatcg aaactccaaa tagagttgct aggatgtatg aagagatatt ctctggtctt
    23401 agtgaagaac caagagacca tttaaaagtt ctttttgcag atgaaaaaca tgaagagtta
    23461 gtattggtaa aagatattcc attttattca tgttgtgagc atcatctagt tcctttcttt
    23521 ggaaaagctc atattgctta tttaccaaaa ggaggaagac ttacagggct ttctaaactt
    23581 gctagagtaa tagatacatt ggcaaaaaga ccacaattac aagaaagaat cactaaaaat
    23641 gctgcagata taataatgga agaattacaa ccttatggtg tcttagtagt agtagaagct
    23701 gaacatatgt gcatgacaat gagaggcgta aaaaaaccag gttcaaaaac tgttacatca
    23761 gcagtgagag gaatatttga aaaggacatt gcatctagag ccgaggctat gagtttgatt
    23821 actatgaagt agcaatttat tatagatata atcatttatt gagataaaat ttaatttaag
    23881 ggagtgtcat taattgtgtt tgattatggt aaaagaactt atataatggg aatattaaat
    23941 gtaactccag atagtttttc tgatggagga gattttaata acttggacat agcaatacaa
    24001 catgcaaaag atatggttga ccaaggtgca gatataatag acctaggagg agagtctaca
    24061 cgcccaggtc atagctatgt agactcagac gaagagctaa gaagagtaat ccctgtaata
    24121 aaaaaactta aacaggaatt ggatatacca atatcaatag acacttataa ggcagatgtt
    24181 gcagaagaag cattaaaatt aggtgtcact atggtaaatg atgtttgggg tcttagaaaa
    24241 gataaaaata tggcttctgt aataggaaaa tatgatgcag aagtgtgtat aatgcataat
    24301 caagatggga caaactatga caaagatata atggagtcaa taaaagactt ttttaaggta
    24361 agtatagaaa tggccatgag ttgtggtgta aaaaaagaaa aaatagtact tgacccaggt
    24421 gtaggatttg gcaaggattt tgaacaaaat attgaagtat tgagaagatt aaatgaatta
    24481 aaagatttag ggtatcctat tttgcttggg acatccagaa aatcagtaat aggaaaagta
    24541 cttccagttg agcctaaaaa aagattagaa ggtacaatag ctactactgt tcttggtata
    24601 agagatggcg tagatatagt tagagtacat gatgtttatg aaaatttaat ggcagctaga
    24661 atgactgatg caatatatag aaagtaggta taatataatg gataaaatat tacttagtaa
    24721 tctaggattc tatggatatc atggtgttct taaagaagaa aattttctag gtcagaaatt
    24781 ctttgtcgac atggaacttt atattgattc aagagaagca ggtcttagtg acgatataaa
    24841 taaatctgta agctatgccg aggtttacaa tgttgttaaa gatattactg aaaataaaca
    24901 atttaatctt ttggaggctt tagcggaaaa tattgctgaa gaagtcttga ataaatttat
    24961 acttataaat ggagttatgg tcagagttag aaagcctgag gctcctgtaa atggtattta
    25021 tgactatttt ggtgttgaga taaggagagc aagagatgaa taaggcttat ttgggaatag
    25081 gtactaacat gggtgataga tttgataatt tatcaagagc atgtgaactt ttgaaaaata
    25141 gtgattctat atataaagta aaagaatcaa gcttatacga aacaaaacca tggggatata
    25201 cagaacaagc agattttcta aatatgtgtg tagaaataga aacagagttt gaaccttatg
    25261 agttgttaga atattgtcaa gaaatagaga gagaactaca tagagagaga atagttcatt
    25321 ggggaccaag aactatagat gtagacgtac tattttttaa tgatgtagtt tccactgatg
    25381 aaagactaac aatacctcat ccaaggattc aagatagagc tttcgttttg ataccactta
    25441 tggatttaaa tgaagaactt ataataaatg aaaaaacaat aaaagaacat ttaaacctcc
    25501 tatctgctga agaaagagaa gaagtaaagg agcttgtagg ttatgaaaga aaacctattt
    25561 aataataatt caaagataga gataaataca aataacaaaa agataactgt aaaaaaagat
    25621 aaacttataa tagctggacc atgtgcaata gagagttatg agcaactttt ggagactgct
    25681 aaatttgtaa aaagtcaggg agcaaatatt ttaagaggtg gagcatacaa gccaagaaca
    25741 tctccaaatt catttcaagg attaaaaaaa gaaggtcttg aaatattaaa agcggttaaa
    25801 gatgaagttg gtatggctgt tattacagaa cttatggatg taagagatat ggacgaactg
    25861 tatagtataa gtgatataat tcaaattggg tcaagaaata tgcaaaactt tactctttta
    25921 agtgaagtgg gaaaacaaaa taaacctgtt atgttaaaaa gaggaatagc atccacaatt
    25981 actgaatgga taggtgcttc tgaatatata gcaatagaag gaaatagtaa tattataatg
    26041 tgtgagagag gtataagaac gtataatgat tatactagaa atacattaga tttagctgcg
    26101 gtacccatta ttcaaaagga aacaggtctt cctgttgtgg tagacccaag ccatgccact
    26161 ggagttagat atttggtaaa acctatgtca ctggcatctt ttgcatgtgg agcagatgga
    26221 ataatggtag aagtacatcc tgacccagaa aatgctttgt cagatggtgc tcaatctctt
    26281 tgctttaatg aatttgaaga tttaatgaaa tcaattaata attattaaaa gaggcaagag
    26341 cctctttttt attcgtaatc gttgctaagg taaatttata agttataatt tgatagttag
    26401 attatttaat ataaataaag atttaatatt gatagaatta atataaaaaa taaaaatttt
    26461 ttgattaata agaaggatat caataaaaaa agtcgaattg tgtatattga gtgattgaca
    26521 aatttagaat atagtgtaga atagaaatgt taacacaaaa aattacacca gaaggtgata
    26581 atatgaatga tataaaagac attattgaag aaataaaagc tagatgcgat atagcaagta
    26641 taatatctga atatatgaat ataaaacaat ctggagctaa ttacaaagga ttatgtccat
    26701 ttcatggcga aaagacacct tctttttata taaatacatc aaaacagatt tataagtgtt
    26761 ttggttgtgg tgaaggtgga gacgtaataa attttgtcat gaaaatggaa aatttggatt
    26821 ttatggatgc tgtaaaaatt ttagcaaata aatgcggtat agaaattaac actaacatga
    26881 atgaagaaac taggattaaa atagaaaaat ctaagaaatt tcaagatatt catacggaag
    26941 cagctagatt ttatctttca aacttattgg gaagcaaaaa tcttgggtat gaatatttaa
    27001 gaataagagg actagatgat aaaattatta agaaatttgg tttaggattt tctttggatt
    27061 catggaattc acttatgaat actctaatca gtaaaggata taaaaaacaa gacttattgg
    27121 agtgcggtct aattgcaaaa aatagagatg gaactaattg ctatgataaa tttagaaata
    27181 gagttatgtt tcctatattc gattatagag gaaatataat aggatttggg ggaagagtat
    27241 tagacgattc tttgccaaaa tacttaaatt ctccagacac attaattttt aataaaaaac
    27301 agaacttata cggattaaat tttgctagaa aaaatttaga aagtaagact atagtcttag
    27361 ttgaaggata tatggattta atttcgcttt atcagtatgg tataaaaaat gtagtagcaa
    27421 cccttggaac agccttgact gaacaacaag gcattttaat aaagagatat gcggacactg
    27481 ctattatatc ttatgattct gatgaagcag gaataaaagc gactttaaga gctatagata
    27541 tacttactaa attaggtatc aatgtaaaag tgttagactt aaaagatgct aaagacccag
    27601 atgaatttgt aagaaaatac ggacttagtg attataaaaa agctatggat gtttctactc
    27661 actacataaa atataaaata gatcatctta aaaaagaatt taacattcaa aaagatgaag
    27721 aacgagtgaa gttcgcaaaa gaagcttcta aaattataaa acaattgaca agtcctgttg
    27781 aaattgattt ttatacaaag tatttaagca atcaaataga tattaatgtc gagtctataa
    27841 agagagaagt ttatggtaaa aattacaata agccatataa taacaaaaat caaaagaaaa
    27901 tagaagaaaa agtaattgaa aaagtcgaag tcagacaaga cggaaaacaa ttagttgaag
    27961 aaactctaat aaaaataatg ttagaagaca aaaaaattag agaaatagcg ttacttaaag
    28021 tagaagaaag tgatttttta ttgaaagaaa gtaaagaaat tttaaattac atgattaaaa
    28081 atcaagaatt ggacaaaata actattgaca aattgaaaag tttaaacata tccgaagaat
    28141 acttaaaaga attaaattca atttctttga atagtataaa cttagagaac acaaaagaaa
    28201 tagaaggaat aataacaaat atcagaaaaa actcattaga agagcaaatt aatagcttgt
    28261 tgagagaaca acaagaatta gaaaataata atgatatgaa agaggtagat ggtagagtta
    28321 tggaaattgc tttaaaaata gttgagataa ataaaatttt aaagagcttg tagagaaagg
    28381 aggtttgagt gtggaaaata aatcgaataa aaaagagtta aaaaaggtta ctgctaagac
    28441 attaatagaa aaaggaaaaa agcaaggttc tctgacactt gcagagataa tggaagcttt
    28501 ttcggagact gaacttgata aggatcaagt agaaaatctt tatgagactt taggaaattt
    28561 gggaatagaa ataacagaaa caaaaaacta taaagctgat atagattttt cggttgctga
    28621 tgacgattta agtataggcc acttagatga agatgcagag gcaatttcac atgatgactc
    28681 ttctgcaata gaaatagaaa ctgtggattt atctttacca aaagggataa gtatagatga
    28741 ccctgtaaga atgtacttaa aagaaatagg aaaaattcct ctacttaaac cacatgaaga
    28801 agtagaattt gctagaagga tgcacgaagg tgatgagata gcaaaacaaa ggttagttga
    28861 agcaaactta agactagttg taagtatagc aaaaagatat gtaggaagag gtatgctttt
    28921 cttggattta atacaagagg gaaatttagg tcttataaaa gcagtcgaaa aatttgacta
    28981 tacaaaagga tataagttta gtacttatgc aacatggtgg ataagacaag ctataacacg
    29041 tgctattgca gaccaagcta gaactataag aataccagtt catatggtag agactataaa
    29101 taagctaata agagtatcaa gacaactact tcaagaactt ggaagagatc caaaaccaga
    29161 agaaattgca aaagaaatgg aaatgacaga agataaagta agagaaataa tgaaaatagc
    29221 tcaagaccct gtgtctttag aaacaccaat aggagaagaa gaagatagcc atttaggtga
    29281 ctttattcca gatgatgatg ctccagcccc agcagaggca gcagcatatt ctttattaaa
    29341 agaacaaata gaagatgtac ttggttcatt aaatgataga gaacaaaaag tattaaagct
    29401 tagatttggt cttgaggatg gtagagccag aactcttgag gaagttggaa aagagtttga
    29461 tgtaactaga gagagaataa gacagataga agcaaaagca ctaagaaaac taagacatcc
    29521 aagtagatca aaaaaactta gagattattt agactaaaaa gatacatcat tgatgttttt
    29581 gtgataaatg ccatgagata agtgatatct catggctatt ttgtagtaaa gatttaattt
    29641 tatttttgta gttatttcag tctaatatct ataacttgga ttaaatccta taaataaagg
    29701 gaattgaaag gagaattata tttgaaatta acagatagat tattgaaaat agcatcatta
    29761 gtgtcagatg gtaaaaaaat agctgatata gggacggacc acggatacat accagtatat
    29821 ttgttaaaag agggaaaagt accatttgca gtgctagcgg atgttaataa aggacctctt
    29881 gacaatgcac ataaagaagt catacagaac aatcttttag ataaagttga tttacgattg
    29941 ggttctggta tagagatact tgaaataggg gaagtagaag aagtgataat tgctggtatg
    30001 ggtggaatct tgataagtga gcttctcgag gctaaaaaag aagtagcaca taatgtagaa
    30061 aaattaatat tacagccaat gcaagcacaa gaagaactta gatattacct tttaaacaac
    30121 ggatatgaaa ttttagaaga agttttagtg agagaagatt ttagaatata tgagataatt
    30181 gttgccaaat atacaggtaa aaatactata atagaagatg agatatatta tgaggttgga
    30241 ataaagcttt tagaaaacaa agattctctt tttaatgatt ttatagaaaa aaagataaag
    30301 acatatagtt ctatagttaa taaattagaa gggaaaaatg gggaagctat agataagaag
    30361 agaaaggaaa gcgaagttgc aataaaaaaa cttgaaaatt taataaaata aataggaggt
    30421 atatatgcta ttaaagagtc taactagaaa aattgaaaaa aagtatcctc taaacttagc
    30481 agaggattgg gataatgtag gtcttatagt tggagatttt gatatggatg taaaaaaggt
    30541 attggtatct ttagaagcta atgaagatgt tattgatgaa gctatatcca aaaatataga
    30601 tttaattgtg acacatcatc cttttatatt tgggaaaatt aataaaatta atagtggaga
    30661 tttaaaaggc agacttatcc aaaaacttat taaaaatgat atatcacttt attctatgca
    30721 tacgaatttt gatatagcat ttgatggtct aaatgactat tttatggaaa ttatggaatt
    30781 tggtaattca aaggttttag atataactaa aagcgaaaat ttgtataaat tagcagtata
    30841 cgttccacat aactattcag atgaacttag aaaggtttta agtaattcag gagcaggtca
    30901 tatagggaat tatagtgatt gtacgttttc aatagaaggt gaaggacaat ttaagccatt
    30961 agaaggctct aatccgtttc ttggaagtgt aaatgatata gaaactgtaa atgaagttaa
    31021 aattgaaact gtagtaccac agaagctttt aggtggggta ataagttcta tgttagatgc
    31081 acatccttat gaagaagttg catatgattt gtacaagtta gaaaataaag gtgagatatt
    31141 tggactaggt agaataagta aacttgataa aagtatgaca ttagaaagtc tttcaaggaa
    31201 aataaaagag aaattaaata tgaaacacat aagagtagtt ggaaacctta gtacagacat
    31261 aacgaaagta gccgttgtaa caggagctgg ttcagaattt gtaaaaaaag ctaagagaca
    31321 aggtgcagaa gtactaatta cgggtgatgt aaagtatcat gaggcacaag atgcacttga
    31381 tattggaatg tgtatagtgg attgtggtca ttttgataca gaagatatat tcaaaaatgt
    31441 tatgaagaga tttttagatg aatttagtga aattgaagta ataaaaagta atgtatattt
    31501 gaatccattt agtactattt agcagtgttt gataataaaa atactatata aaaaataaat
    31561 ttgagtttaa ttagggggat aatgatgaaa gtagcagtaa tttttcatag tgtttgtggg
    31621 agtacatatt tattagctag agaatataaa gaagcgcttg aagaaatgaa tatcgaggta
    31681 gatatattta gagtaagtga cgaagttgca aaaacacttc ctcaatatta tttgataaat
    31741 tccaaagaat acaaagacga atttgaaagt ataaatgtaa ttaaatctgg aaaagaaata
    31801 ttagattatg atgctatatt tatgggttca ccaacatatt atggaaatgt atctggacca
    31861 atgaagatgt ttatggatag tttttctgat atttgggtag gtactccttt gagtggtaaa
    31921 atatttggtt gttttgctac agcaggttca caacatggag gaggagaatt agctttgcaa
    31981 gctatgaata tatttgccca acatatggga atgactcttt tgtcagttcc atgttcagta
    32041 agaggaggtt atccagcgta tggaatactt catatagcag gagataattc tgatataaga
    32101 ccaaatgatg atgccaagat aggtataaga gattacctaa aaagacttaa catataagaa
    32161 tatcatataa aaatacctaa aagcgactgt atatactttt aggtattttt acttaattat
    32221 aaacgtgaag ctttaccaga aatatggtca atttctattt taacaacagt tgttaagttc
    32281 atatccttct ctatgtactt catgccttct tcaaagaatc ctttagaata tttttttatt
    32341 atttctacaa gagcattttt cttttcttca ttttctacag tgcaagcttt tccaaaaact
    32401 atagcactag aataagtagt actaaattta tctggaataa cactttcatt agcaactact
    32461 aaaaaagaaa ctttgttgtt tttcgatata ttatctaact tatgcccatt tcttgcacaa
    32521 tgaaagtaaa tagaatcatt aaagtataca taatttacag caactccata aggataacca
    32581 ttttcagata tagtggaaaa agtaccaaat tcaccatttt ttaatacttc aacagtatct
    32641 tcttttgtca tttctctttt tttcaatctc atttctttaa acataatatc atcctttcta
    32701 attttgatgc ttaaattaaa ttttagaaca tttataaaga ttatgctatg ttttttacaa
    32761 attttaagaa aaaaatatag tttaagataa aaggtagtga aagagaagct atttggaggt
    32821 ttatttatga tattattgtt agagtagtat aaacaatact aataaattta atagctggtt
    32881 tcttgttatt taagatagta agcatgtcta taagaagggg aagttatcga gggtagaagt
    32941 tgttatttat aactatatta taaagaaaat attattatat aaaataataa atagtaaaga
    33001 ggtatcaagt tgaatataat acttaatacc tcttcaaaat taagttataa aagtgttgtg
    33061 ggaattgtcc tatacatggt aaggagataa taaagaatgt atctttaaag tctcaactaa
    33121 acagcaaaat ttatccaaca aacatacaat aaatgcttct ttgtatctag gtggcttaac
    33181 agtaagtggc cacatgtgtt ttaaaattat gtccgtttct ttttcattta tttgaaaaaa
    33241 taaagttgca ttttttaatg cttctctagg gtgagtaaat ccatgtaatc cttttctatc
    33301 acctttgtaa tgccaatcat aaagaaaaaa gtcgtgtagt aaagctcctc taataacact
    33361 ttgaatatca acacttaagt gcaatttttt acataataga taactataat atgctactga
    33421 caaactatgt tcaagacatg aaacattgcc atgatgagga aatgttctca taagttgaac
    33481 tttttcagaa gaaagtatct cagaagcaca ttcattaaaa tatttgattt cagcttttga
    33541 caacattatg aaaatctcca ttcttagtaa tatataaata ctatacttaa acgataatat
    33601 gaaatatttc ttatcctaca aaatataata cccaataata caagtaagat accttttatg
    33661 gtattttaaa gtaagagaga ttttattagg ctaaacaatg aaaaaattaa aagtaaagct
    33721 atagcaatat atagtatttt tccaagagga ttgccaaaat ttatagtcca tccaatacca
    33781 aaccttttat taaccattaa tgatgggtca tttggattat tatacatgat gcctgctatc
    33841 caatacttct catcttcttc tggagaataa gatgagttga attttaaatt tggggaatta
    33901 atatatgttg ctattaaata aattataact aaaagcgttg taatattcat agcagttgat
    33961 agttttgttt ttattgataa aagagtagta aaaaactgtg ttgtcattat aatcatcaat
    34021 attaaaaaca aataaccaat tttatttaaa tatttaatgt tttcaactct actttcttca
    34081 attctatttg tatctatctt aatccttgac tttatggatc caatggaagt tatatataat
    34141 aaaaccatta aacaaaagtc agtaccgatt aatttaaata cattaagaaa tgatttatct
    34201 ataaaaacat caggagaccc atttatgttc caatgagtgg caattgagtc tggcaattga
    34261 ttataattaa atgctataaa aatactcatt cccaaagtaa atagtatcgg tattaaatag
    34321 attattttaa atttttttat tattttattt ttcttattta taaaatccat atctactatt
    34381 aattttgaat ctatatctat atgacctaat ttagaataaa tttctgattt aagcttctta
    34441 gcttttttat gtgtatgaat atatagtata ctttcatata ataaaaaacc tataatacca
    34501 ataaaatagg aaagttccaa tttattaaat gtaaagacca gaataaaaac taatataaag
    34561 tctataataa aacctaaaga taatagtttt ttaaattctt tatctaactt tataaagtaa
    34621 ttataatttt tatgctcttg gtctatagaa actccataga tgtgtttttt acctacaaaa
    34681 tagggcataa aataaaatgt agcatacatc aaaattagaa ctggaaatat tgtaaataga
    34741 cattcaatct tactcataat atttcacctc atacttgtca aataaacttg aacttagtga
    34801 caaaaattct tctttactaa tacctgataa ataagatttt gccaccaata attccaagtc
    34861 aagtttattt ttttgacgag ttgtattatc tattttcatt ggtagttgag ctacaatagc
    34921 accttttctt ctatcaatat ttaagaatcc ctcttctttt aattcattgt aggatttgtt
    34981 cactgtatgt aaatttacac caatattttc agccatactt cttacagatg gaagtgattc
    35041 atttattttc aattctcctg aagctatagc cttagttatt tcttctttaa tttgtacata
    35101 aatagattta tcactattaa aatctaaatt taaaaccata aaaaacctcc attatgcttt
    35161 ataaatgaaa acgattgtta tatacatagt ataacataaa aaataataac aaacacaaaa
    35221 tataagttta tattttgtaa aaattgtaat ttaaaaaata acataatttt tattaaaata
    35281 atacagatac tatttataga tagaaataaa aattagaaag gagaaaaaat atgttaacat
    35341 atttaatgaa aagacaaatg cataaaaaag atgatggaat gagtaaacca atgatgatta
    35401 caggagctgt aattacaggt gtaggagcat atgctgtata taaaatggta aaaagccata
    35461 aatctaattc tcaaatggtc aatacaaatt attatggaaa tagcgattat gatgattata
    35521 aatatgacga atttgattat gactgtgatt gtagagacac aaaacaagat tatgaatatg
    35581 aagaattaaa aaaaaaagta aaagaattta attcaagaag aataaattgt gaaaattgtc
    35641 cacatgtttc tcaagaagaa atgatgcaat atgtaaatag tgttaaagat gtaaaggaag
    35701 agattgattt acatgaagat gctaaaaaag ataatattta caaagaagat gaatacaaaa
    35761 aatatgaaga agaataagta atattttgta taactgaaca aatagtaatg tcaaattatt
    35821 taatatctag ttttaatatg taagttgtaa cttaacttga aagaatcagt atcattcttt
    35881 agatagttaa gtaacaactt acattttatt tttataaatt ttaatagttc tttgtgacta
    35941 ttttgtaatt ttagaaatat tttagtatcc agggtaattg tgtataataa aacttgtata
    36001 gatagatagt atgaaattaa gcagatagtt taaaattaaa atgaaaagaa aggaaatagt
    36061 tatgttaaag ttgttgatag ttgaagatga tagtactata tcttttggaa taaagtatgc
    36121 tttagaacaa gaaggattta gtatagatat atcaaaggat ttatctagtg gaaaagaaat
    36181 gatttcttct aatgaatata gcatgatact gttggatgtt acattgccag atggaactgg
    36241 ttatgagctt tgtcaatata taagaggatt ctctcaggtt cctataatat tcttgactgc
    36301 ttgtgatgaa gaagtaaata ttgtaatggg tctcgatata ggtggagatg attatataac
    36361 aaaacctttt agaattagag aattaatatc cagaataaaa gctgttttaa gaagaaaagg
    36421 taatacttca gaagaaaata agaaaatact taaatttgga gatttaagta tatatacatt
    36481 agaagctaga gtatataaaa atgataaaga aatatttctg acatcagttg aatataaact
    36541 tcttttaatt cttatacaga ataaaaatac tgttttaagt agaactcaaa tattggaaaa
    36601 actttgggat gtaacttatg attttgtaaa cgacaatact ctaactgttt atataaaaag
    36661 gcttagagaa aaaataggtg atgatttatg tgaaccaatc tacatagaaa ctgtaagggg
    36721 gattggttat aaatggatag gaagtgaaaa tagtgtttct atataatcca gaagtgaaga
    36781 agtttttaag taagtatgtt actttgatgt ttattgttat cataatatct attggattta
    36841 gcataatcaa cgtgtcctta actaaagaga tggttgtaag aaataatcag gctataatag
    36901 gaactttatc aagcaagtat ccaaacttag aaagtcagat agtagacata ataactcaag
    36961 ggaagtctat ggagaacata gattatggtc agaaaatatt aattaagtat aattatgata
    37021 aatctattag gataaactct gagcctatta tttctaaatt agttttagag actatcaaaa
    37081 taaatataat attagtatgt ataatattta ttttgatatt tgtgttggtt gttagatatt
    37141 ttaagtcaat ttacaatgat ttatcagata tgactaagta tgtttactat agttcagagg
    37201 gtaagccatt tgatatgaaa aacaagaatc aagaagggca gataggtctt ctaaaaactg
    37261 aactcttaaa gatgacgaca attcttaatg aaaaggttga acttttaaaa agagaaaaga
    37321 tatttttaaa caatacaatt tccgatatat cacatcaatt aaaaactcct atgacttctt
    37381 taataatgtt gaatgattta ttatataatg atgtaccata cgaagtaaaa atcgattttt
    37441 taaataagat aaaaaatcaa ttaaatagaa tggattggtt gattaaaagc atgcttaaat
    37501 tatcaaaggt agaagcaaaa gtgataaatt ttaaaaaaga taaggtaaaa tttagtgaac
    37561 tcatacatag agcaatgcta tctatgaaaa tacctatgga gattaaaaat caaaaactga
    37621 ctatagaggg aaatgataat gtatcttata ttggtgatat tgattggtcc gtggaagcat
    37681 tagtgaatat aatcaaaaat tgtgtagaac atactcctga atttggaaat ataattataa
    37741 cctataaaga aaatccatta ttttctgaac taataataaa agatgagggt gaaggtatag
    37801 ataaaaaaga tataccacat gtatttaaac gattctatag aggaagaagt agttcaaaag
    37861 aagatagtgt aggaataggt cttgcaatgt caaaatctat aatagaaagc caaaatggcg
    37921 atatatatgt aaacagcgaa aaaggtcagg gtacagaatt tcatataata tttcataaaa
    37981 tgtatgatag tgactaatct gtaatattta attcacttta tagtaatagc caaaacctaa
    38041 tatatgaagt atagaatata aatattggag gtagttatgg aaattttaag agtagaaaat
    38101 ctaactaaaa gttatggtaa aaatgaaaca aaagttgatg ctataaaaaa tgtaagttta
    38161 tcagttgaaa aaggaacgtt tatagcaata acaggtccaa gtggaagtgg taaaagtacc
    38221 ttattacatt tattaggagg agttgacaga ccaactagtg gtaaggttta tataaatgat
    38281 gttgatatct ataatttaaa aactaaggat ttggcgatat ttagaagaag aaatataggt
    38341 cttatatatc aattttacaa cttaattcca gtcttaaatg tgaaggaaaa catattgctt
    38401 ccagcagagc ttgacaatag agatattgat agagaatatt ttgatgattt aatgaaaaca
    38461 ttagatctaa ttgatagaga aaagcacctt ccaaaccaat tatcaggagg tcaacaacag
    38521 agaacctcta taggacgtgc attaattaat agaccttcta taatattagc agatgagcca
    38581 ataggtaatt tagatagtaa aaattcaaag gaaatattag aattgttaaa attgtcagta
    38641 aaaaaataca atcagacact aataatgata actcatgaca aaaatatggc acttcaagct
    38701 gatagaatta taagcataga agatggaatc ataaaaagtg atgaggtgat gtagatgaat
    38761 ttatatacat cactaaccat tagatactta aaacaaaata aaagaagaac cattgtaacc
    38821 ataatcggta taatactttc aacagcttta atttgtggta taggaaatat atttgaaagt
    38881 tttatggatt atcaaatgag agaaactata aagaatgatg gaagtttcca tgttacattt
    38941 tatgatgtaa atagaaaaaa tgcggaatat gtaacgaaat cagctgaaat agaaaaacat
    39001 gcatttacca aacaacttgg atattctaaa ttagaaaata gtgaaaatgc tatattaagt
    39061 ataaagcaat atgataaaaa tgcaataaac ggatataaag tttctataaa agaaggtaga
    39121 tttccagcta aggaaggaga aattgtttta agtgaaagta ttattaatct tatagatgat
    39181 aagttaaaaa taggagataa gataacttta aaagtagggg atatgtttga ctcaagcaag
    39241 aaaaagattg atagtatgac atttcatgaa ggtgattata tagcaaatga aaaagataga
    39301 acttttaaag ttgttggtat aattgaaaaa cctgggtttg aacgttataa tggaattgca
    39361 acagctatag cctactttaa tcctatgaat aattacaatg atacaattaa tgtatcagta
    39421 gcagtaagaa atcctaaaga tgtgtataag ataagtaata aaataagtaa aaatatatat
    39481 tcaaataaag ataaagaagc agtttccgat atgataaaat acaatgaaca tttattgagg
    39541 ttacaaggtg ctagtaagta ttcaaatata aatagctcta taaagtctat aattatagca
    39601 gtaactattt tagtaatcat atgtacaatt gctacagtgt acaattcctt tagtatttct
    39661 ataaatgaac gaaaaaaaca gtttgggata ttaaattcta taggtgctac atctagtcaa
    39721 attaaaagat tagtttttat agaaggtatt ataattagtt taattggtat accaatagga
    39781 ctaatatctg gaacagtagc tatagacata ttgtttaaaa ttataaataa gtattttact
    39841 gaatcggttg ttactaagat gagtttgcag atagtgtata atccaataat aattattgtt
    39901 agtataataa tagttttgct tacaatcttt atatcaattc tactacctgc aatatcggct
    39961 tctaatattt ctcccctcaa tgtgattaaa aatactggag agtataaagt gggcaaaatc
    40021 aaaagctcaa gacttattaa aatgattttt aaaactgaag gagtacttgc atataaaaat
    40081 attaggagaa atagaaaaaa atttataata actctttttt ctttaatggt aagtataata
    40141 atatttatat cttttagtgg ctttacatta cttttactaa gaggtgaaga aattagaaat
    40201 tctcaaagaa aatatgattt gtatttaact gcaaagaaca cagctaggtc tgtagaggat
    40261 actataagtg aactagaaga tattgatggg ataaaaaact ttgaattggc aacaggacaa
    40321 tatgtgtcta taagagtaag tgaaaataaa ataaataaat ccaaggaaga tttaatcagg
    40381 aaatattatc aaaaatataa gattggagat agttatgaat atgatttcag taataatgaa
    40441 ttaatttttc caggagattt tgcagtaaaa aacaaaataa ataacttagt acagggctct
    40501 tttaataaag agagagcaat tgaagaaaat ggtgttatat tagttagaaa aagtgcattt
    40561 gaagaaaatg ggaagaaagg tgtagtagaa cttaccaact ataaagtagg agatacagta
    40621 aattgtgaat atcttgatga aaatgcctta agtaaaaaag taaaggtcaa gattcttgct
    40681 ataactgacg aagaacgtct aggtcttggt tatcaaaata tggggttaca atttataact
    40741 tatgatgaag ttgctaaaaa tttaaattta aaattaagta aaagccttat atttattgat
    40801 tcaggaggaa gcattaagac taagaaggaa atagaagctt tggcaaataa aaataatttt
    40861 aacttttatg atgaaagtgc aagcaatgaa agtgaaaaac aagatttaaa ggttattaaa
    40921 atatttgtat atggttttat agtagtgata tctttagtga gtgttacaaa tattctaaat
    40981 acagtaagta caagtataaa tctaagaaaa agagaacttg cagtaataca atctataggg
    41041 gttactccta aaggatttag gaaaatgata tatttagaaa gttttatata tggtatactg
    41101 tcattgttgt ttggaatacc aattagtatt ggtataattc taattatgaa taaacttatt
    41161 tcaggtgtaa ttgaattttc tccgattata ccgtggacag ccatagttat ctgtatagta
    41221 agtgttttta taataacttt tatagcaggg tatataccaa tgagtaaatt aaataaagaa
    41281 aatataa
//
LOCUS       DAEMIK010000015        40801 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEMIK010000015 DAEMIK010000000
VERSION     DAEMIK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.015"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPK87_000683"
     tRNA            27..101
                     /locus_tag="KPK87_000683"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPK87_000684"
     tRNA            116..200
                     /locus_tag="KPK87_000684"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPK87_000685"
     tRNA            210..293
                     /locus_tag="KPK87_000685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KPK87_000686"
     tRNA            317..391
                     /locus_tag="KPK87_000686"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KPK87_000687"
     tRNA            416..492
                     /locus_tag="KPK87_000687"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            500..575
                     /locus_tag="KPK87_000688"
     tRNA            500..575
                     /locus_tag="KPK87_000688"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:533..535,aa:Gln,seq:ttg)
     gene            584..659
                     /locus_tag="KPK87_000689"
     tRNA            584..659
                     /locus_tag="KPK87_000689"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:617..619,aa:Lys,seq:ttt)
     gene            662..750
                     /locus_tag="KPK87_000690"
     tRNA            662..750
                     /locus_tag="KPK87_000690"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Ser,seq:tga)
     gene            754..829
                     /locus_tag="KPK87_000691"
     tRNA            754..829
                     /locus_tag="KPK87_000691"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:787..789,aa:Phe,seq:gaa)
     gene            836..909
                     /locus_tag="KPK87_000692"
     tRNA            836..909
                     /locus_tag="KPK87_000692"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Met,seq:cat)
     gene            924..1000
                     /locus_tag="KPK87_000693"
     tRNA            924..1000
                     /locus_tag="KPK87_000693"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:958..960,aa:Ile,seq:cat)
     gene            1018..1094
                     /locus_tag="KPK87_000694"
     tRNA            1018..1094
                     /locus_tag="KPK87_000694"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1052..1054,aa:His,seq:gtg)
     gene            1103..1178
                     /locus_tag="KPK87_000695"
     tRNA            1103..1178
                     /locus_tag="KPK87_000695"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Lys,seq:ttt)
     gene            1186..1259
                     /locus_tag="KPK87_000696"
     tRNA            1186..1259
                     /locus_tag="KPK87_000696"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1218..1220,aa:Cys,seq:gca)
     gene            1267..1343
                     /locus_tag="KPK87_000697"
     tRNA            1267..1343
                     /locus_tag="KPK87_000697"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Arg,seq:acg)
     gene            1356..1431
                     /locus_tag="KPK87_000698"
     tRNA            1356..1431
                     /locus_tag="KPK87_000698"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1389..1391,aa:Val,seq:tac)
     gene            1507..1920
                     /locus_tag="KPK87_000699"
     CDS             1507..1920
                     /locus_tag="KPK87_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536875.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVYGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2229..5660
                     /locus_tag="KPK87_000700"
     CDS             2229..5660
                     /locus_tag="KPK87_000700"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG2536876.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKISKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5902..7830
                     /locus_tag="KPK87_000701"
     CDS             5902..7830
                     /locus_tag="KPK87_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG2536877.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8283..8747
                     /locus_tag="KPK87_000702"
     CDS             8283..8747
                     /locus_tag="KPK87_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG2536878.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8754..9248
                     /locus_tag="KPK87_000703"
     CDS             8754..9248
                     /locus_tag="KPK87_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG2536879.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9264..10289
                     /locus_tag="KPK87_000704"
     CDS             9264..10289
                     /locus_tag="KPK87_000704"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG2536880.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10276..12723
                     /locus_tag="KPK87_000705"
     CDS             10276..12723
                     /locus_tag="KPK87_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG2536881.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12903..14276
                     /gene="radA"
                     /locus_tag="KPK87_000706"
     CDS             12903..14276
                     /gene="radA"
                     /locus_tag="KPK87_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG2536882.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14279..15349
                     /gene="disA"
                     /locus_tag="KPK87_000707"
     CDS             14279..15349
                     /gene="disA"
                     /locus_tag="KPK87_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG2536883.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15438..16532
                     /locus_tag="KPK87_000708"
     CDS             15438..16532
                     /locus_tag="KPK87_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG2536884.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16661..18838
                     /locus_tag="KPK87_000709"
     CDS             16661..18838
                     /locus_tag="KPK87_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536885.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(19024..19794)
                     /locus_tag="KPK87_000710"
                     /pseudo
     CDS             complement(19024..19794)
                     /locus_tag="KPK87_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(19912..21030)
                     /locus_tag="KPK87_000711"
     CDS             complement(19912..21030)
                     /locus_tag="KPK87_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2536886.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKKKAGFPKF
                     KSKKINRFSYKTNFTNGNIVYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKFAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRYINASINILKEGLRLITIQNK"
     gene            21970..23136
                     /locus_tag="KPK87_000712"
     CDS             21970..23136
                     /locus_tag="KPK87_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG2536887.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYIAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMAKGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFESKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            23158..24804
                     /locus_tag="KPK87_000713"
     CDS             23158..24804
                     /locus_tag="KPK87_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG2536888.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMENESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            24901..26274
                     /locus_tag="KPK87_000714"
     CDS             24901..26274
                     /locus_tag="KPK87_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2536889.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VISASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            26719..27672
                     /locus_tag="KPK87_000715"
     CDS             26719..27672
                     /locus_tag="KPK87_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG2536890.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARVFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            27710..28672
                     /locus_tag="KPK87_000716"
     CDS             27710..28672
                     /locus_tag="KPK87_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG2536891.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            28697..29683
                     /locus_tag="KPK87_000717"
     CDS             28697..29683
                     /locus_tag="KPK87_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG2536892.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDEDVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            29696..30742
                     /locus_tag="KPK87_000718"
     CDS             29696..30742
                     /locus_tag="KPK87_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG2536893.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            30909..32639
                     /gene="lpdA"
                     /locus_tag="KPK87_000719"
     CDS             30909..32639
                     /gene="lpdA"
                     /locus_tag="KPK87_000719"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG2536894.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            33056..36004
                     /locus_tag="KPK87_000720"
     CDS             33056..36004
                     /locus_tag="KPK87_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2536895.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            35982..36446
                     /locus_tag="KPK87_000721"
     CDS             35982..36446
                     /locus_tag="KPK87_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2536896.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEMDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVNLSEESVSDLLNSKLKN"
     gene            36559..36861
                     /locus_tag="KPK87_000722"
     CDS             36559..36861
                     /locus_tag="KPK87_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2536897.1"
                     /translation="MGVTNVAKKILVACGTRVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            36923..38287
                     /locus_tag="KPK87_000723"
     CDS             36923..38287
                     /locus_tag="KPK87_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2536898.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPVASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADILQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVIFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            38322..38795
                     /locus_tag="KPK87_000724"
     CDS             38322..38795
                     /locus_tag="KPK87_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536899.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINNGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            38967..39617
                     /locus_tag="KPK87_000725"
     CDS             38967..39617
                     /locus_tag="KPK87_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG2536900.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECSSTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            39791..40723
                     /locus_tag="KPK87_000726"
     CDS             39791..40723
                     /locus_tag="KPK87_000726"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG2536901.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKYNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFDNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
BASE COUNT        16280 a         4573 c         7497 g        12451 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatatt ggggattagc caagtcggta aggcacatga ctttgactca
      541 tgtatgcgta ggttcgagtc ctgcatcccc agccaaacag tatggtctat tagctcagtc
      601 ggtagagcac ctgactttta atcagggtgt cccgcgttcg agtcgcggat agatcaccaa
      661 tggagaggtg tccgagtggt ttaaggagct ggtcttgaaa accagtgact tcgaaagggg
      721 ccgtgggttc gaatcccacc ctctccgcca aatgcccaga tagctcagtc ggtagagcag
      781 gggactgaaa atccccgtgt cggtggttcg attccgcctc tgggcaccaa aatgtggcgg
      841 tatagcttag ttggctagag cgttcggttc atacccgaaa ggtcataggt tcgactccta
      901 ttaccgctac taaaatttaa tgtgggccat tagctcagtt ggttagagcg cccggctcat
      961 aaccggtagg tctggggttc gagtccctga tggcccacca cattaaatta acttatggtg
     1021 ggtatagctc agttggttag agcgccagat tgtggctctg gaggtcgtga gttcgactct
     1081 cattatccac cccaacttat atggtctatt agctcagtcg gtagagcacc tgacttttaa
     1141 tcagggtgtc ccgcgttcga gtcgcggata gatcaccaaa aaggcggcga catagccaag
     1201 tggtaaggca gtggactgca actccttgat ccccagttcg aatctgggtg tcgcctccaa
     1261 taaaatgcgc tcatagctca actggataga gtgtctgact acgaatcaga aggttagggg
     1321 ttcgagtccc tttgggcgca ccatatgaat agcgagggat tatagctcag ctgggagagc
     1381 acctgcctta caagcagggg gtcacaggtt cgagccctgt taatcccacc agtgcaaacc
     1441 tctagtaaaa ctactagagg ttttattttt gattgaaaaa atttttatat ttaggaggta
     1501 aaaaatttgt atatagtaat aggaaatgaa atagttgata gtgaagaact taaaataatt
     1561 atagataaaa atagtgaatt taaagttgaa aaagatttaa gtaaaagcac aaaaagagaa
     1621 gatgtaatag catatcagtt aagtatagat ttaaattacc tagattctct tataaatgag
     1681 caatgtaatt tagccagtct atctgatgaa gagaaatttg atgaatatat gactttgtct
     1741 gatgaacttg cactggattt agaagagtta atgccaaaat atacaataat aaatgctaga
     1801 gcatataaat tagacgaggt atatggaatt gtaaaaataa tactagctgt agcgtatgca
     1861 gatttagggc acttaaaact ttctgatgta gtaaagaggc tatctagaca agtagattag
     1921 gatatgaata tgaactatat taaaaaaatg tataaaatct tacgtaaaga tgaaaataat
     1981 tatgtaaaca tagaaaaaaa cagtataaaa tcaagtcaat tgtagaaatt ttcacataaa
     2041 ttcatctgaa attctataaa aaatgcctta attttaatga ggtaaatcga gtttacacag
     2101 tctaaaaatt acttatattt ataaatttat cttattaatt aaggaacttt cttaaacatc
     2161 ttcaaaaata aagtatttta gtctataatc tttaatatta cacaaacaat aaaggagaag
     2221 aatagagtat gcttaaaaag tttaataaaa tactagtggc taatagaggc gaaatagcta
     2281 taagaatatt tagagcctgt tcagaattgg ggataaaaag tgttggtata tacagtaagg
     2341 aagataagta tggattgttt agaacaaaag cggatgaatc atatttgata ggagaaggta
     2401 aagggccaat agatgcatat ttagatatgg atggaataat agacctagca aagagaaaaa
     2461 aagttgatgc tatacatcca ggatatggtt ttctagcgga gaatgcagaa tttgctcgta
     2521 aatgtgaaga gaatggaatt acttttatag gaccatcatc taaggtaatg aatatgatgg
     2581 gggataagat taactctaaa aaaatctcaa aagaagttaa tgttcaaact ataccaggag
     2641 tagaaaaagc aataagaagt actgaagaag caaaggaagt tgcaaacaaa ataggatatc
     2701 ctgttatgat aaaagcatct aatggcggtg gcggtagagg tatgagaatt gtacatcgcg
     2761 aagaagactt agagcttgaa tatgaaactg cttgtagtga atcaagaaaa gcttttggag
     2821 aagatattat atttatagaa aaatatatag ctgatccaaa acatatagaa gttcaaatat
     2881 taggtgataa ttatggaaat atagttcact tatatgaaag agattgttca gttcaaagaa
     2941 gacatcaaaa aataatagaa tatgcaccag ctttttcgtt agatgataaa gtaagaaaag
     3001 agatatgtga agatgcagtt aagttatcaa aacatgtggg ttattctaat gcgggaacat
     3061 tagaattttt agtagatgca aatggtgggc attattttat agagatgaac acaagagtac
     3121 aggttgaaca tactgtaaca gagatggtta caggaataga tatagttcaa agtcaaatac
     3181 taatagccca aggttacagc ttagatagtg aagaaattaa tataaaatca caagatgatg
     3241 tggaaataag aggttactct atccaatgta gaataactac agaagaccct aagaataaat
     3301 tcatgcctga tacaggtaaa attcaagttt atagaactgg ttcaggattt ggtataagac
     3361 tagatggagg aaatggattt acaggggcaa acataagtcc tcattatgat agtttattgg
     3421 ttaagacaat atcttgggat agaacattcc aaggagcaat aaataaaact ataagatcta
     3481 taaaagaact tagagttaga ggtgttaaaa ctaatgtagg attcctagtt aatgttttaa
     3541 ataatcctat attctcaaat ggtaaatgta gtactaagtt tatagatgaa aatccagatt
     3601 tatttgaaat aacagaaagt aaagacagag gaacaaaatt acttcaattt ataggagatg
     3661 taattgtaaa tgataatgct tgtaaagaaa aaccattatt tgatgcatta catgacccaa
     3721 gaatggataa ggatggttca aaatcagaag gaagtaaaat acttttcgat aagttaggta
     3781 aatctgcata tatagaaaag ataaaaaatg ataagaaatt actattaaca gatacaacaa
     3841 tgagagatgc acatcaatca ttattagcaa caagaataag aacatatgac ttattaaaag
     3901 ctgctaagcc aacagaaaaa tatcaaaagg atttattctc acttgaaatg tggggaggag
     3961 ctacttatga tgttgcatat agatttttaa aagaatctcc ttggcgtaga ttacaaaagc
     4021 taagagaaga aataccaagc ataatgttcc aaatgttgct tagagcatca aatggagttg
     4081 gatataaaaa ctatccagat aatgtaatag aagaatttac aaaagaatct gctagacaag
     4141 gtatagatgt atttagaata tttgattcat taaactgggt tgaaaatatg aaaccatcta
     4201 ttaatacagc tttagaaact ggaaaaattg ttgaagcaac tatgtgttat acaggtgata
     4261 tactagataa gactaaaact aaatacaatc tagaatatta tattaaaatg gcacaagaat
     4321 tagaaagctt gggagcagat ataatagcaa taaaagatat gtcaggtctt cttaaaccat
     4381 attcagctta cacactagtt aaagagttaa agaaaaatgt aaaagcacca atacatttac
     4441 acactcatga tacaagtgga aatggtgtag caacttgttt aatggcgtca gaagctggag
     4501 tagacataat tgatgctgca ttagaatcta tggcaggact tacaagtcag ccatcactta
     4561 atgctatagt agaggcactt aagaatacag agagagatac aggaatagat ttatttggat
     4621 atgatgagtt aggaaaatat tataaggatt taagaaaagt ttataataaa tttgaaagtg
     4681 atttaactaa ttcttgtgca gaaatatata attttgaaat accaggaggc caatatacaa
     4741 acttaaaacc tcaagctgat agtttaggat tagttaatag atttgatgaa gttaaagaaa
     4801 aatataaaga agctaatgaa gttgtaggag atataataaa agtaactcca tcttcaaaag
     4861 ttgtaggaga cttagctatc tttatgacta aaaataaatt agataaagat aatataatag
     4921 aagaaggtaa aaatttatca ttcccagatt ctgttgtgga ttactgtaaa ggtatgattg
     4981 gtcaaccaga aggtggaata ccaaaagatt tacaagaggt agttctaaaa ggtgaagaag
     5041 ctataacagt aagaccaggt tcattattac cagcagaaga ttttgatgaa atagctaaat
     5101 atctaaatga aaagtatgat ataaatgcta atataagaaa tgttataagt tatgctttat
     5161 atccaaaagt atatgaagat tatatcaaac atctacaaca ttataatgat atatctaaac
     5221 ttgaaagtga tgtattcttc tatggattaa ataaaaatga agaatgtgaa gtagaaatag
     5281 aagaaggtaa ggtattaaca ataagattag ttgaaattgg tgaagttaag gaaaatggat
     5341 ttagaactat aggatttgag ttaaatggaa tggttagaga agtagaaata aaagataaga
     5401 atttctctgg aaaaattaat aatgttgaaa aagctgatat gaatgaccct cttcaaattg
     5461 gagcaagcat accaggaaaa gttataaaaa taatggttaa agaagaagat gaagtaaaag
     5521 caaatcaacc attaatcgtt atagaggcta tgaagatgga aactataata gttgctaaga
     5581 ctgatggagt aattaagtca atcaaagtaa aagaagatga tatggttgaa gataaacaat
     5641 tattaatgat aatgaagtaa tttaattaaa ataaattaaa aagcactctg aaatgtttaa
     5701 taaaaaatgt tttagagtgt ttttatttat tttaattaaa tatttgttaa aaaaaagagg
     5761 acaattacct ccagatgtag aaataaagct agtaaggaaa atgttatcta aaaattctaa
     5821 atatagtaaa tattttccat aatatttctt aggaaataac agcaaagata aaatacataa
     5881 atttaaaagg tgggtgctta tatgaaggtt tatgatagta aaatgttgag aaatgtagcg
     5941 gtattaggac atagcggatg tggaaaaaca aatctaatcg aaactatagc ttacacagca
     6001 aacactaata agatacctaa attaacagac aaggtaaata tgacttatag catgggatta
     6061 atacctatag agtataatga ttataagttt aacttattgg ataccccagg atattttgac
     6121 tttagcggag acgttgtgtc atctcttaga gcaagtgatg cagcaataat agtaatagat
     6181 gcaacagcgc ctattcaagt tggtacagag aaatcattag aactaactga gagtattcct
     6241 aagataatgt ttataaataa aatagataat gaaaaggcaa gatacaaaga tgcaatagca
     6301 atgttaagag aaaaatacaa taataagata gttcctatga taagtcctat atacaaagat
     6361 aaaaactttg taaagttaca taatgttttt gaaaacatag atgatttaga aggtgaattt
     6421 aaggaacaag ctatgagtgt taaagaagca ttaatggaac ttatagcaga aactgatgac
     6481 caaatattag ataaatattt taatggagaa gaactaacta cagaagagat acaaaaaggt
     6541 attattatag gtatacaaag aggtgatatt atacctgtaa tatgtggttc aactataaat
     6601 aatataggca caaaggaaat attagataca atatcaagtt atttagaacc tatctttaca
     6661 gaggaaagca aaccatttag gggattagta tttaaaacta tggttgaccc atttgttgga
     6721 aagatgtcat atataaaaat tactgagggt gtactttcta aggataaaga tgtttttaat
     6781 ataaataaaa atgtaaaaga aaaaatagct aatatatata cattgagaaa tagtgaatta
     6841 gtagaaatag aaaaagctaa agctggtgat atagtagtta taactaaagt taattctcta
     6901 aaaacaggag acactatatc tgcagataaa gatgcagaag ctttagaaaa gatagatttt
     6961 cctaaaccac aaatatatta tgcagtaact cctaagaata aaggtgatga agaaaaagta
     7021 gcatctgtat taaataaact agtagaagag gaccctacat tacattggta tagaaataca
     7081 gagacaaaac aagcactttt aggaggtcaa ggagaacttc atataaaaac aattaaaaat
     7141 aaaatgaaag ataagtttgg tgttgatgtt gaattgaatg accttaaagt tccttataga
     7201 gaaactataa aaggaacagc tgatgtacaa ggaaaacata aaaagcaatc aggtggtcat
     7261 ggtcaatatg gagacgtaaa aataagattt gaaagatgtg aatctgattt tgaatttaca
     7321 gaagaaatat ttggtggttc tgtaccaaaa caatatatac cagcagttga aaaaggttta
     7381 aaagactcta tgcaaaaagg tatattagca ggatatcctg ttacaaacat aaaagctacg
     7441 ctttatgatg gttcatacca tgatgtagac tcttctgaaa tggcctttaa aatggcagca
     7501 agtgcagcct ttaaaaaagg tatggaagaa gcacacccaa tactactaga acctataatg
     7561 aaacttaaga taactgttcc agaagaatat atgggagatg tcatgggtga tataaataag
     7621 agaagaggaa aaatatttgg aatggaacca gatgacaaag ggaaacaaat aatatttgca
     7681 gaagctccac aagcagagac atttaaatat gcaatagatt taagagcaat gacacaaggt
     7741 agaggatact ttgaaatgga attagagaga tatggagagg ttccatcaca gtttgcagaa
     7801 aaaataattg ggctagctac tgcaaagtaa tgaacaaaat aaatagaaaa ataattaata
     7861 tatattatta aaaatatttt tagggtttta actaggtata ggtttatgtt aggtaaactt
     7921 atacctagtt tttatacatt tttgatgaat aaaaaaatat ataatacttt cttaaatatt
     7981 ttaataaaaa gaaagtattt ttttattatt atttttacaa atgtttagta ataaagatga
     8041 gaaggtatat ttaaaatata aagtatttaa agttctgaaa tctaatcatg aagcaaaaaa
     8101 ttagcatttg gttatcaata aatataataa ataaaaactc ataattaatg aaagaataaa
     8161 ttcatatcta aatttctata aaaaatttaa atttatacaa tttagggctt taaaaattta
     8221 aataataggt gtaaaataat agtcaaagaa agtcaaaaat aaaaaggtgt gtggtgatat
     8281 taatggcaac tatgacagat ataattgaaa aatttattaa agatttgatg gaagaagata
     8341 atagcataca aattcaaaga aatgaactag caaacttgtt tagttgtgca ccatcacaaa
     8401 taaattatgt cttaacaaca agatttacaa tagatagagg atattacatt gaaagtaaaa
     8461 aaggtggcgg aggatatgtt caaatagaaa aaatacgaaa aagtaaagat gggcatatta
     8521 gagaactact aaatgaaaaa ataggaagtc agatatccta taaaaaagca aaagaattat
     8581 tagaaggttt aaaagagtcg gatttgatta acgaaagaga attaaaatta atattatatg
     8641 caatagatga taaatcattg tgtatgccaa tatatgaatt aaaagagaag gtaaggtcta
     8701 atattttaaa gaatataata ataggattat ttagtataga ggagtgataa tttatgctat
     8761 gtcaaaaatg caataagaat aaagcatctg tatattataa taagatagtc aatggagaaa
     8821 aaacagagat gtatttatgt agtgagtgtg caaaagagaa tacagaaatg aattttaact
     8881 tagatatgcc tttttcaatg atggatatat tttctaattt aggttttcaa cctaaaaaag
     8941 aacttgagga aaagctagtt tgtcctaaat gcaatacaac atatagtgag tttaagaata
     9001 atgggagatt tggatgtagt gaatgttata atgcatttag tagtcaagta aatccaatgc
     9061 ttcaaaatat acatgggcat atagaacata caggaaaagc acctaaaaaa tctttttata
     9121 agataagtgt agaaaatgag attaaagaac taaaagaaga tttagataga gctataaaaa
     9181 atgaagagta tgaattagca gctcaattta gagataaaat taaatattta aaaggttcta
     9241 ttgactaaga ttggagtgat attatgaaag agaacatagt catgaaatca agagttaggc
     9301 tagctagaaa tttaaataac tatccatttc caaataaatt agataaagaa tgtgccatgg
     9361 aaataataga aaaagtaaaa aatgcgttta ttaattctaa cttagaacaa aaagaagaat
     9421 ttgatttcta taaaattgaa gatttagacc aatctaaaaa aatgcttatg gttgaagaac
     9481 atataataag cccagattta gcagaaaatg ataaaagtgc agttatagta aaaaaagata
     9541 aaactataag tataatgata aatgaagaag accatataag gatacaaact atatgtgatg
     9601 atttaaactt agaatatgca tatagtgtag caaatgaaat agatgatttg ttagaatcaa
     9661 gtttagaata tgcctttaac acaaagctag gttatctaac atcttgtcca actaatacag
     9721 gaacaggaat gagagcttct gtaatgatgc atttaccagc attaagtcaa ctaggctata
     9781 tggatgaact ttataaaatt tcatcacaaa taggtatagc aattagaggg atatatggag
     9841 aacgaacaga agcattggga aatatatatc aaatatctaa tcaacttaca cttggaagaa
     9901 cagagtctaa tattatagaa aatgtaagtg gacttacaaa agatgcaatt tcaaaagaaa
     9961 taaaagctag agaaatattg caaaaaaaat taggtataaa actagaagat aaaatattta
    10021 gatcgatagg gacacttgaa aattcaagag taatgagtag cgcagaggca atgagccatc
    10081 tatcaaatat aaaaatggga atagaaatga actatataga taaactagat ttaagggcta
    10141 tagaacaact tatgataggt atacaaccag cgcatcagtc aataatgtat aagagtgatg
    10201 atgttgagaa tagagatata aatagagcaa catatattag agaaactttg gaaaaattaa
    10261 gaggagggat gaattgtgaa cttcaataga tttacacaaa gagctaaaaa ggcaattgat
    10321 ttagcgtttg aatctgctaa aagtctagga cacaatattg tcggaagtga acatatactt
    10381 ttaggtcttt taagagaaga agaaggtata gctgctaaag ttctaagtaa agttggattt
    10441 acagaagctt atttagaagg caaaatagtt gatatggaag gtaagggaga agaaatctca
    10501 gaagatatag tattaagtcc aagaagtaaa caaatacttg aattatcagg aatgtttgca
    10561 aataaattaa aaacaaatta tattgggact gagcatatat tattagcaat tattcaagaa
    10621 ggtgaaggaa tagctaataa gattttaaat tatgctggag taaatgatag gactttagcc
    10681 caattaacaa ttgatatgat gggtattagt gataaaaatc aatacaaagc agaaaatagt
    10741 tacacgggca accagaatca agcagaatct aaagtacttg ataagtatgg aagaaatctt
    10801 acactatatg caaaacagaa taaaatagac cctgtaatag gtagagaaaa agaaatacaa
    10861 agagtaatac aaatattaag tagaagaact aaaaacaatc cagtgttgat aggagaccca
    10921 ggtgtaggga aaacagccat agctgaaggt ctggctacaa atatagcctt aggaaatgtt
    10981 ccagaaacac taaaaaacaa aactttatac tctttagaga tgggttcatt attggctggg
    11041 gctaagtata gaggagaatt tgaagaaaga attaaagaag ttgtagatga agttgttaaa
    11101 aatggaaaca taatcttatt tatagatgag atgcacacaa taataggggc tgggtctact
    11161 ggagaaggct ctatagatgc ttcaaatata ttaaagccag ccttagcaag aggagaaata
    11221 caagttatag gtgcaactac aattgatgaa tatagaaagc atgttgaaaa agattctgct
    11281 ctagaaagaa gattccaacc agttatggta gatgaaccaa ctaaagagga ctcaataaaa
    11341 atattagaag gattaagaga taaatatgaa gctcatcata aagttaaaat aactgatgat
    11401 gctataaagg cagctgtaga attatcaact agatatatat cagacagata tctgccagat
    11461 aaagctatag acttgattga tgaagctgca tcaaaagtaa gattaaaaga aaatacacct
    11521 ccttcagaaa taaaaaaatt agaactagaa atagaaaata tagataaaga aaaagaagaa
    11581 gcagtaagat gtcaagactt tgagaaagca gccaaaataa gagatgaaca aggtctactt
    11641 aaaaaacaat tagaagatgt tagagaaaga tggaacaagt catctaaaca ttcagattta
    11701 gttgatggag aagttattgc tgaagtagtg ggcttatgga caggaatacc agttaataaa
    11761 attcttgaag aagaggctga tagacttttg agacttgagg aaatactgca caatagagtt
    11821 ataggtcaag aacaagcagt aaaatctatt tcaaaggcaa tcagaaggtc aagagcaggt
    11881 cttaaagacc ctaatagacc aattggttca tttttatttt taggtccaac aggagtaggt
    11941 aaaacagaat tatctaaggc attagcagaa gtgcaatttg gagatgaaaa tcaaataatt
    12001 agaatagata tgtctgaata tatggaaaaa catgctgtgt caagaatgat aggttcacct
    12061 ccaggttatg taggtcatga tgaaggtggt caattaactg aaaaagtaag aagaaatcca
    12121 tattcagtta tcttatttga tgagatagaa aaagctcatc cagatgtatt taatattctg
    12181 ttacaaattc tagatgatgg tagactaact gattcaaaag gaagaactgt agattttaag
    12241 aatactattg taataatgac atcaaatgtt ggagcatcta caattggtag acaaaaaact
    12301 ttaggattta gtatagctaa aggagacgaa gaagaaaaat ctcaatatga aaaaatgaaa
    12361 gaaaatataa tgggtgaatt aaaacaaaga ttcagaccag agtttttaaa cagaatagat
    12421 gatataattg ttttccactc actaaatgaa aatcatatat caaaaatagt acttttaatg
    12481 gcagcgaaac tacaagagag attaaaagaa atggatataa aattagaaat gagtgatgag
    12541 gctgttaaat taatttctaa atctggattt gacttagaat atggagcaag acctcttaaa
    12601 agagctttac aaaaagagtt ggaagatgag ctatctgaag caatcttaaa aggtaatgtg
    12661 aaaaagggaa gtaatgtagt agcaaaagtc aaagatgaaa aaatagtttt tgaaactaaa
    12721 taatttaata gagttgcatt ttaaaatatt gcaaatataa gttttataat ctaaacaaaa
    12781 ttttaagggc aatgaatcta taataattca ttgtccttaa ttattttatt taatctttac
    12841 atattgtctt tcagatatgc taaaattata gtatagaata aaaaattaag gatgataaaa
    12901 gtatggcaaa aataaaaaca aaatatgtat gtcaatcttg cggatatgaa acagctaaat
    12961 ggcttggtaa atgtccagaa tgtacaaaat ggaatacatt tgtagaagaa atagaccaaa
    13021 aaagcaccaa aaaagaggtc tttataatag ataaatcttc atctaaacca gtcagtataa
    13081 actcaataga aagtaaagaa gaagagcggt ttacaacaga tataaatgag ttagatagag
    13141 tgcttggagg aggtatagtt aaaggctctt tagtgcttgt tggtggtgac cctggtatag
    13201 gtaaatcaac acttttaatc caagtatcta gtaatgttgc caatttagga aaaactgttt
    13261 tatatataac tggagaagaa tcagaatctc aaataaaaat gagagctaaa agattaggta
    13321 ttaactcaga aaatttatat atatttgctg aaaataattt aagtataatt gaatcttatt
    13381 tagaaagtgt aaatccagaa ttaataattc tagactcaat tcaaactgtc ttcagtccag
    13441 aaatatcctc ggcaccagga actgtcagcc aaattaaaga aggtacttca aaattcatga
    13501 aaatttctaa aaaaatggga atttcaacgt tcatagttgg gcatgttaca aaagaaggtt
    13561 cactagcagg accaaaatta ttagagcata tggttgatac tgttctatat tttgaaggag
    13621 aaagatataa tacttataga ttagtaagag ctgttaaaaa tcgatttggt tcaactaatg
    13681 aacttggagt atttgaaatg agggatttag gattagtaga acttgataat ccatctaaaa
    13741 ttttaatctc tgaaaaacca aaagatgtag caggttcagt aataatatct actgtagaag
    13801 gtacgagacc aatgttactt gaattgcaag cattagtttc tcctacaagt tttggtatac
    13861 caaaaagaac atctacagga gtcgattata atagagtagg tatgttatta gcagtattag
    13921 aaaaacgtgt tggtttgcaa atacaaaatc aagatgtcta tataaacata gttggaggaa
    13981 taaagataaa tgaaccctca attgatttag ggatagccat ttctgtagca tctagtttta
    14041 gaaatatacc gatagatgaa gatatagctg ttacaggaga agtgggactt acaggagaag
    14101 ttagagcagt aagctttata gaaaagagaa tagctgagtg taaaaaactt ggatttaaaa
    14161 agatagtagt tcctagaagt aattatgatg ttgtaaaaga gaccaaaggc atagaaatat
    14221 ggccagtaga taacttaaga caagctataa atatagtact tgggaggaat caataaatat
    14281 ggagaatttt ctagataata aaaatatgct atatgcatta aaaatgatat ctcctggaac
    14341 tccacttaga ttaggtctaa acaatgtact aagagctaag actggtggat taattgtaat
    14401 tgcaacaaac gaagatgtaa tgaaaatagt agatggagga tttgctataa atgcagaata
    14461 ttcaccatca tatctatatg aattagctaa aatggatgga gctatagttt taagtggtga
    14521 tgtaaagaaa atattatttg ctaatgcaca acttatacct gactatttta tagaaacatc
    14581 agagacagga acaagacata gaacagcaga aagagtagca aaacaaactg gtgctatagt
    14641 cataggaatt tcacaaagaa gaaatgttat aacagtttat agaggaaatg agaagtatgt
    14701 agtcgaagat atatctaaga tatttactaa ggcaaatcag gctatacaaa ctctggaaaa
    14761 atataagaca gtattggacc aagctgtaac aaatttaaat gccttagagt ttaatgattt
    14821 ggtaactatt tatgatgttg cattagtcat gcaaaagatg gaaatggtaa tgagagttac
    14881 aagtataatt gaaaaatatg tgatagaatt gggtgatgaa ggaactttag taagtatgca
    14941 attagaagaa ttaatgggta caaccagaat agaccagaaa ttaatattca aagattataa
    15001 taaagaaaac acagaaataa aagaacttat gaaaaaggtc aaaaatttaa attcagaaga
    15061 actaatagaa ttggtcaata tggcaaaact attagggtat agtggttttt cagaaagtat
    15121 ggatatgcct ataaaaacaa gaggttatag gattcttagc aaaatacata gactaccaac
    15181 agcaataata gaaaacttag taaattattt tgaaaacttt caacaaattt tagatgcatc
    15241 tattgaagaa ttagatgagg ttgaaggaat aggtgaaata agagcaacat atataaaaaa
    15301 tggactcata aaaatgaaac aattagtctt attagataga cacatatgat gaattttaaa
    15361 tgaattttac tacttttttc ttgaatataa tatacacttt ggtctataat agataagtat
    15421 aactaggggg tgaagcattg ataaggaaag taacaagaat attgtttatt ttacttggat
    15481 ttacaatagg tataacaacc tatttaacat taatgaaaga ttttgaaata ttaacatttg
    15541 gaaaagaaac atatggttat atcgcagctg tagtagcagg tataataata gccattttag
    15601 gatatcttat agaaccatgg gttgtaaata aagtaaaaga aattgctaaa atagtggata
    15661 aagaattgtc aaaatatcct cagactgata tattgttagg gtctatgggt cttatagtag
    15721 gatttgttat tgcgtattta ttaagtggat tagtaaatag aataccaata gtaggtggaa
    15781 tactatcctt actattatat ttattcctag gatatcttgg aatgaaagtt gcgcttaaaa
    15841 gtaaaaatga tttatttaat attggaaaac taggaagatt agcaaatcct attaaggata
    15901 aagataagga taaagaaaat aaaaaagaag taaaagccat tcctccaaaa gtattagata
    15961 caagtgtaat aattgatgga agaatagctg atatttgtaa gactgggttt atagaaggaa
    16021 aacttattat tcctgctttt gttttggagg aactaagaca tattgcagat tcttcagatg
    16081 atttaaaaag agtaagaggt agaagaggtc tggatatatt aaatataatt cagaaagaat
    16141 taaatataga agttgaaata agtgagagag actttgatga tatagcagaa gttgatagta
    16201 aattattaaa gcttgctcag gttttaaatg gaaaagttgt tacaaatgat tataacttaa
    16261 ataaagttgc tcaattccaa ggtgttgaag tattaaatat aaatgagtta gctaatgcta
    16321 taaaaccagt tgctatacca ggtgaagata tggttgttca agtggttaaa gaaggaaaag
    16381 aggctcaaca aggtgttgca tatctagatg atggaactat gatagttgtt gatggaggaa
    16441 gaaaatatat gaatgagact ataaaagtac tcgttacttc tgttcttcaa actcctgcag
    16501 gtagaatgat atttgcaaaa cctaaaaatt aatatgttca aagaaaccta taatttgggc
    16561 tgtttatttt aaatagatgg tccaaattta tttatttata acgataatgt ttaatgtatt
    16621 cttaaagatg gggatttaaa tcagaaagca gggagaattt atgagaaaaa agattatatc
    16681 tttcttagca acatttatca taatattaac atcagcaagt cagtatagtt ttgcagatga
    16741 tatatcaaca cgtggaaaag taatatttat agatatgaat agaactagta tgagtaatat
    16801 gttaagaata aaatcattaa gagaagaact agacaataga gggtatattg gcttgatgaa
    16861 tataagaggc gataaaggaa gtgatgatag aaggtcatat gctagtatgg gtgctggtgg
    16921 tagagctaat gtggctaatg aagaagatat taattttgaa agctcgagta aggatagaaa
    16981 tatagttttt gaatcagcta caggaaaaag tgcaaaaggt ataaataatt taactataaa
    17041 taagtctata aatgaaaatt taaactttgg agagtatggt tctgtattag ggtcattagg
    17101 acaaagttta tctgataatg gattaaaagc atctgtattg ggtaattcag atataattga
    17161 aaatggtcaa cttataaaaa atagaaactt atgtctaact gcaatggatg aatatggaag
    17221 aatacctaat ggaaatgtag atactataaa taaaaaagac ttatctatgc cttatggaat
    17281 atcaactgat tatgataaat tgattgtaga gactaaagaa gcttacaaaa ataatgatgt
    17341 gatttttgtg gagcttggag acacatatag attagattta tataaaccaa atttaaatga
    17401 aaaaacatat gagagtatga aagataacat agaaaaaaat atagatatat atttaaaaaa
    17461 tatattttct atggttgaag aaaatgacac tgtctacata gcaagtgcat ttccaagtga
    17521 tttagattat aaaaataaaa gaagactttc gccaataata aaattaaatg gagaagggaa
    17581 aggtatatta tcatcttcaa ctactagaag agaaggtatt gtaacaaatt tagatgttgg
    17641 ggttgaaata ttagacaact ttaatataaa aaatcaaaat atggttggaa gaaaatatga
    17701 acttattaac agagatgata ataaagaatt tttgatggat gaatatcaaa agatagtatc
    17761 tatttcaagc ataaggtcta caatactgaa tggttttgta agtatagttt tcttgtcatg
    17821 gatagttgct atgattgcaa ttttatttag aaagcatata tcaaaaaatt ataaagaaac
    17881 aacatttttt atattaaagg aacttttaaa aataggaata gtaatgccac tgtcatttat
    17941 gataacacca ataatgaatt tcaaaacacc attagcaata tctttaggaa taattataat
    18001 aacacttact atatatctta taagtaaagt tttaataaag aacgatttaa aaaacatgct
    18061 gttttttact ggattaacca tagtaattat ggttattgat gctgggtttg gctcatattt
    18121 aatgaaaagc aatgttatga gttatgattg tataattgga gctagatatt atggagtagg
    18181 aaatgagtat caaggtgtgg caatcggtag tgcaatattt acatttgcaa ttttacttac
    18241 atataaaaat ataccaaagt ggtctataat agttttttct ttggttatct taattacaag
    18301 tgcatctcca attatgggag caaatgttgg aagtgctatt tctgaatgtg ttgcattttt
    18361 actatttata ctattgatat ataatgtaaa gatagatttc aaaaaaatcg tattattagg
    18421 tatagcagta gtatttgtat taggagtttt tgtagcaata gatatgattt tgggaagtaa
    18481 ttcacattta ggcatgtttg taaaagaaat ttactttaat gggccaggag aaataataca
    18541 aacatttagt agaaagatag agatgaattt aaagctagct caaaccagtg catgggtaaa
    18601 tatactgtta actggaatag gtgtaatttt agttcttatg attaatcaga ttagatactt
    18661 taagcaactg atggatgagt atccaattgt atttaaagga tttatagcat caatagcagg
    18721 atgtttagta actcttttag tgaatgactc tgggattgtg tcatcagcta cagcctttat
    18781 ttatgtaatt gtacctatga taacattaag tataaatctt acagcactaa aagaataagt
    18841 actgtatgaa taatatgtaa atattaatac attaatttaa cttctttatt tacaaaataa
    18901 agaagaaagt ttataaaaaa taaattataa taaaaaagag tttatctcaa aattgattat
    18961 tctaatcaaa ttttaagata aacttttttt attataaaga taaataacat ataacctcat
    19021 attttaggta gatttttttc ttatatgttt aggagtgcat ttaaattttt ccttaaaaat
    19081 tctgtaaaat aattttgtat tagtaaatcc atgagcttct aatatactgc ataaggtatc
    19141 atttgtgtta attaaatctt tataaatatg agaaatacgc acatcattta aatactctaa
    19201 gtaagtttgt cccatatact gtttaaattt tctacaaaaa tattctggtt gtaagtgtgc
    19261 taccttagcg attttgttaa tagaaatagg cattttgtaa ttaatctttg tatattctaa
    19321 aactggttca aattttgaca aatcaagtac tgtttttttc atatttttat tacctacaga
    19381 tattttaaag tttgtatata attcaaataa aagttcaaat aataagcttg taaagagcag
    19441 attagctgca ggaggagaac taagtttaat ttctctcatg ttttctaata taagtttaac
    19501 tttacttgaa ttttttttat agttattgtc attggcatta accttaaagt caaaatagca
    19561 atttttaaaa tcagggatat atttgtttag aaaagacaat ggtacttgca ataatataga
    19621 ggtattttga taaatacatc tagttgagtg aataattcca gaattaatga acatacactc
    19681 attagctttt aaattataag tgtaaccctc catgttaatt tgtaaatcac cagaagttat
    19741 ataaattatt tctaatgagt tatgccaatg atttgctaca taactaccac tatcctgaac
    19801 ctcccatgac taaagtcaca gggttcctgc ttcatagaat tttgcatact aaaatatgtc
    19861 ttactaattc tccacaggct atccccgtag ttcctacggt tcttacatac gttatttatt
    19921 ttgaattgtt attagtctta atccttcttt taatatattt atacttgcat ttatatatct
    19981 atcgtgagtt tcattacaac taggacaaat ccattctctt atatttaagt ccttcacttc
    20041 ctcgttttta tacccacatt tattgcatat ttgtgaactt gcaaagaatt tacctacctt
    20101 tacaatttgt ctaccatgcc aattagcctt gtattctaat tgacgaataa attctgacca
    20161 tgatacatct gaaattaagc gagatagttt acgatttcta atcatattct ttacttgtaa
    20221 atcttctatg caaataatat cattctcttt tattaattta gtagataatt tttgtagaaa
    20281 atcctttctt tgattagcta tatgttcttg aagtcttgca acttttaatc ttgctttatt
    20341 tctatttaag ctaccgattg tttttctcga taattctctt tgtaatttag caaatttgtt
    20401 taatgacttc ttaagatact taggattttc tataaattct ccacaacttg agatacaaaa
    20461 ttctttaatt cctaaatcta aacctatatg attattagta ttttcaaatg cttcaatatc
    20521 tacatcagta cagcataatg atacataata tctaccactt ggctctttag atatagtagc
    20581 attaagtatt ctacctttag gtacttgttt atcccttatc ttaaccatac caagtttagg
    20641 tagttttata tgttgaccac aatacacgat atttccattt gtaaagttag ttttatatga
    20701 aaatctatta attttctttg atttaaattt aggaaatcct gccttttttt taaagaattt
    20761 tttatatgca ttatctaaat cttttaaggc gttttgtagt gaaaatttgt caggttcctt
    20821 aagccaattt aattcttttt ttaaattggt caaatcagaa ctacactgtt tataagtaaa
    20881 agtttcttta tcgtttttat aaacttctat tcttttagca agatatttat tatatacaaa
    20941 tctacaacat ccaaaagttt tgttaattaa ctcttgttgt tttttatttg gatacattct
    21001 aaacttataa gctttttcca ctactatcac ctcactttaa ttgttttttg atacactttt
    21061 taatgatttt atcatattta atatattata taatgctgtg atgaacaatt tattaatcac
    21121 ctatcataat attaataaat gtaattattt aaaacaatta tttagaaggt tttatattta
    21181 attttaaata acatatttaa gaaccgtaag tcttaacttt tatcattttt gaggttgtcg
    21241 ttcacataag ttcgctacta cctatgcagt tctcttatga acttcttaca ctttcatgca
    21301 agcacagact atatcttatc cttcggcatt atccgttaag gtctacccac ttccactacc
    21361 aatagcttgt agtgtacttc cctcaagagg aatagtcgtt gaagtttctc ctattcggag
    21421 tttacctgct gattgcccat tttaaagtac ttagggttta accttatacc atctaaccaa
    21481 ttttttctac tttcgtaact ttcacactta ggtttatttc atccttatgt tttagtttgg
    21541 ttagctttag gggtttccag caattcaagt agtgttggat aggttctatc ctatctctac
    21601 atatatgttt ccctatatgc tgactatttt aactactaac tcatgactaa agtcacgagt
    21661 gtgcgtagct attttttaat caaatagttt gaaattaaca tctaaattat caataggttc
    21721 aattatttca tgtgaaaaat tatttctgtt cataataata tcctccttat aattttaaat
    21781 tataaatagt aaatttataa ttttcaatat taaaaagata atatagacta ataaattaga
    21841 taataaacat acttaagaaa acggataaaa tcaaatataa taactgtata ttaaaataac
    21901 ttaaaaaaat tattttatta taagtgcaaa atatacctat ttaaataaga taagagggga
    21961 ggattttatg tgtatataaa aggtgtaaat ttaggaggtt ggttagtttt agaaaaatgg
    22021 atgacctcaa gtttatttga aggcacagaa gctgaagatg aatactattt accaagacag
    22081 ctttcaagag aggcatatga atctagaatt aagactcatc gtagtgaata catcacagag
    22141 agagattttg caacgattaa atctatggga tttaattcag ttcgtatacc agtaccatac
    22201 tttatttttg gagattgtga accattcata ggatgtgtta aagaacttga taaagcattt
    22261 gcttgggcag ataagtatgg gcttagtata ttgatagact tacatacagt tccaggaagc
    22321 caaaatggat ttgataatgg aggaatatca ggtatatgta gctggtcaca aaatccagaa
    22381 tatatagcgt ttacattaaa tgtattagaa agacttgcaa aacgttatgg gatgaggcat
    22441 gaattgtatg gaatccaaat tttaaatgag cctataacag aaagaatgtg gaacataatg
    22501 aatgttccaa atcggtttaa ggctgtagat aaggagatgg caaaggggag caaacctaat
    22561 tcattagagt ttttacgtga tttttatata aaagcatata aggtaatgcg tccttatatg
    22621 agagaggaaa acgttatagt gtttcatgat gcatttgaat caaaagcatg gaaagacttt
    22681 atgagagagg aagaatttaa aaatgttgtt ctagatacac accaatattt aatgttagct
    22741 gaagcagatg gatgtgaaca aagtattgat agctatctca aatatattag agaaaattat
    22801 gctaaagata ttttacaaat gcaaaaatat tttcctgtta tatgtgggga gtggagctta
    22861 tttaattcat atgcttgtgg tatagataca aatggaggtc aatcacctct aaatgggatt
    22921 gaatcaaata tagataaatt atcaaaagac gataaaagag aattatatag gaagatagca
    22981 aaagcacagc ttgatgcatg gagaaatgga agtggtcact actattggaa ttataagtta
    23041 ttactagata cagttaatga agaaggttgg ataggatggg atagttggga cctagtcaaa
    23101 tgtgtagctc aagaatggta tcctatagaa tattaaacta aaattggagg aaaagatatg
    23161 attaaaaacc ctatacttcc aggttttaat ccagatcctt gtatttgtag aaagggagat
    23221 gattattatc ttgtagtttc atcttttgaa tggtttccag gtatacctgt ttatcactct
    23281 aaagacttaa aaaattggga gttatatact catattttga cagatgaaac aaaaatagat
    23341 ttgaagaagc ttccttcatc taaaggaatt tgggcaccct gtttgactta ttgtgaagaa
    23401 gaagatttat tttatatagt ttatggaatt atgaactcta tgaatgcaag atattttgat
    23461 gttgataatt atttaattac atcaaaagat ataaagggag agtggagtga accagtatat
    23521 ctacactcat caggttttga tgcgtctatt tttcatgatg atgatggtaa aaagtggatt
    23581 gcatcattag actgggagac aagagaagga tatgaaaaac ctggtgttat atgtttagtg
    23641 gaatactgta caaaaaagaa agaaatagta ggatatccaa agagaatatg gtcaggggga
    23701 actgatagag gttgtattga agcaccacat ataacaaagc gtggagatta ctattatata
    23761 atgtgtgcag aaggtggaac tggatatggt catagtgtaa ctatgggaag ggctaaaaat
    23821 atatggggac cttatgaaaa agattcaatg aatccaattg taacttcaat tccaggcgat
    23881 ttttatgaaa gacatgaccc tgaccatcta aagcctaaat actataaccc agaatcaaaa
    23941 cttcaaaaat ctggtcatgg aagctatata gaaacaacat caggtgaagt atatttagtt
    24001 catctaacat ctagaccatt tgtaccagag ttaaggtgta ctttaggtag agaaacagct
    24061 attcaaaaaa tgaaatggac aaaggataat tggcttcgaa tggaaaatga aagtaatttg
    24121 gcaaaagaat acgtatcaga aagtaaacta gaagaacatt tagtttcatc tataccaagt
    24181 tttgatgact ttgattcaaa tgaattaggt ttacagtact atgctcctag gatttcacca
    24241 ttatcatttg cagatgttaa gtctagacca ggatatgtaa ggattagagg acaagaatca
    24301 agaacatcat taaataaagt gagcatattg gcaagaaaat taacttcagt atatgcaaga
    24361 ataaccacta agatggagtt ttacccagag gtacatcagc atagtgcggg tttgataatg
    24421 tattatgata atatgaatta tataaattta agaaagtatt atagtgaaac attgggtcaa
    24481 agtgcactat caataataca tcttgaaaat ggagagaaaa cggaattttt aaatactagg
    24541 ataccaataa aagatatacc aatatatctt cgtctgtata tacaaggtcg taagtcatat
    24601 tttgaatgga gttatgatga aaaaaactac cagagaatag gaaaggtatt tgatacaact
    24661 aagttttcag atgagtactg taaatatgga gaatttacag gtacgtttat aggattaact
    24721 tgtgcagatc gtgtaaagca taaacactat gctgattttg atttttttga atatatagta
    24781 gatgaatcaa aagatgtcga ttaataaaat cattactaaa attttcagag caaaggtaaa
    24841 tcttatgctc tgaaataaga aacattagaa ggcaaatctt acaaaaagga gtatggtatt
    24901 atgaatacag aagttaatgt taaagcaact acgattagat atgaaaagat ttcatttttt
    24961 gaaagaattt cttatggttg tggggatttg gggtgcaata tcatatactc agctatgtca
    25021 gcatttttgc tgttttatta tactaattat gcagatgtta gtgcagcagc tgtaggtagt
    25081 attatgcttg tatctaggat actggatggg tttagtgact taacaatggg aattatagtg
    25141 gatagaacta aatccaaata cggtaaggct aggccttgga ttttgagaat ggcgattcct
    25201 tttgctatag cagcagtatt attattttct gttccatcaa atcttggtat cacttctaaa
    25261 ttaatttata tttttattac atataactta gtttctactg ttatttatac agcaatcaat
    25321 gtaccctatg caactctaaa ttcacttata actcaaaacc agtatgaaag aggagtatta
    25381 agtatattta gaatgatatt agccacttgt ggtaccttac taataaatgg acttacacta
    25441 cctctagttg aatactttgg aaataattta tcagcatgga ctaaaacatt ctttgtattt
    25501 ggaatagcat ctataatggt attttttata acatttactg gaaccaagga aagagtaaaa
    25561 gcagttaagc aagataggaa tgaagtaata ccatttaaaa taggtattaa atctttattt
    25621 agaaataagt actggataca aataacatta tgtttagttt gcatatttat tgtatttgcg
    25681 ataaatggtg gttcatccgt atattatgca aagtttattt tgggagatga aaaattattt
    25741 ggtcctataa atatggtttc aaatatatcc caaattatag ctatgtttat ggttgctcct
    25801 tttataaaaa aatttggaaa aagaaatgta ttaatagtag gttcaataat attaatatcg
    25861 tcaaatgtta tgtttataat agttggacaa aactatatag gagtaatatc tgcaagtgct
    25921 ataaagggaa taggaagtgc tggaatagca tcaacaatgt ttgctattgt ttcagatact
    25981 attgaatatg gagaatggaa gactggatat agaactgagg ggcttataaa tagtgcatct
    26041 agttttgggt ttaaagtggg aaatggatta ggttcagcaa tattaggtgc agtattatca
    26101 attggtggtt atgtaggaac atcagctact caaagtgatt tagctatagt atcaataaaa
    26161 gtatgtttta tatacctacc tatatttata acaatattac aaattatcat aatgtctttt
    26221 tataagcttg ataaagaata tagtactata cttaatgaat taaatactaa ataatgaaaa
    26281 agtttcttat aaagtcttaa gtttaattaa ggtctattgt aaatgttccc tataaaaact
    26341 attatatatt tatgattatt gaagaatttt tagagaagcg aataataaaa atataagaag
    26401 actttaaatg atgaaatggt tggtttttta gaagtgattt aataagttat tgttatttaa
    26461 agcattataa gtggacaatt aatttttaat ttatggagat gatatttaac aattagaagt
    26521 aatatataat atgcactaag aagcaatttt tcaactattt atgaaggtgg tggtacaaga
    26581 aaagtatcac cttttttttg ttatacaaaa aaacagttga ctttttatat gaaccccttc
    26641 tataatgtta ctgaaattaa gtaaaatgat tgaacaaatg taaagataat tgaaaagctt
    26701 gattttggag gaaagcaaat gaaaaaaatt ggagacttaa gattaatgat gaagtgttgt
    26761 tcactttatt acgaagataa tttgaatcaa caggaaatag caaatcaatt gggaatatca
    26821 agaccaacaa tatcaagaat attaaaagaa gcttttgaac aaggaatagt aaaaatacag
    26881 atagttgatg ttttgaaaaa tgattaccaa aaaatagaga gaagtttgga gagaaaatat
    26941 aaattaaaag aagtaatagt agtagatgat aaacaagatg cactaacaca aaaacaagaa
    27001 ctagcaagag ctgtatctga atatttaact agagtagtaa aggaaaatga tataataggt
    27061 gtttcaatag gaacaacact aaaagaaata ccaagatatg tggaaaaaag taattgtaaa
    27121 aatgttacat ttatacctct attaggaggg attggagaca atgaaataga tattcatgca
    27181 aatcaaatag cagtaagttt agcgagggtt tttggaggtg attttaaatt attacatgct
    27241 ccagctgtta tgtctgactt gtctacaaaa gagaaacttt gcaaagacga aaaaattaaa
    27301 gaagtactag atttaataga taaaacaact atagcaattg tgggtatagg aaatcctatg
    27361 agtttaaact caacaataat ggcttctgga tacatgaatg aagcagatat agaagattta
    27421 aaaaaataca actcaatagg tgcaatttgt ttacaagcct ttgataaaga aggaaaaact
    27481 tctatacttg aatttaatca aagagtatta ggagtgaaac tggaagattt aaaaaagata
    27541 aaaagaacaa taggtgtcgc ttcaggagat gaaaaaatag aggcaataaa ggcatcatta
    27601 aaagcgaaat ttataaatag tttagcaatt aatcacagtt tagcacttaa attattagaa
    27661 gattgtgact aatagaaaga tagataaaag ttaacaggag ggaatgtata tgtcaatatc
    27721 aaaagaaaca ttgcttgaaa tgtataagag aatgaatcag gctagaaaat ttgaagagaa
    27781 agttagttgg ttttttgcaa gaggtatggt tcatggaaca actcacttat ctgtaggtca
    27841 agaagcgtca agtgtggcgg ctgtaatggc tttagaaaaa ggagatttag tatcattaac
    27901 tcatagagga catagtcaat ttataggaat gggaatagac ctaaataaaa tgatggctga
    27961 acttatgggt aaagaaactg gattttgtaa gggaaaagga ggctcaatgc atatagcaga
    28021 tattgagtca ggtaacttag gagcaaatgg agttgtaggt ggaggtctta caatagctcc
    28081 aggagctgca cttacacaac agtataaaaa aacaggcaaa atagttttat gtagttttgg
    28141 tgatggagca tcaaatgaag gtacttttca tgaaggaata aatttatctt caatatggaa
    28201 attgccaata atattttact gtgagaataa tttatatggt atgtcaacaa gcataaaacg
    28261 tcatatgaat atagaaagta ttgccactag agcagcttct tatggaatag aaggaatatc
    28321 aatagatgga tataacccaa tagaagtata cgaaacagta caaaaagctg ctgagaagtg
    28381 tagaagagga gaaggacctg tattaattga aagtagaact tatagatggt taggtcattc
    28441 aaaatcagat gcaaatgtat atagaacaaa agaagaaata gaatcatgga aagcaaaaga
    28501 ccctattgaa tttttaaaaa attatcttat agaaaataat ctatctaatg aagatgaatt
    28561 agataagatt caagaatttg caaagcaatc aatagaagat gcagtagagt ttgctcaaaa
    28621 tagcccaaat ccaaaaatag agtctttatt agaggatgta tatgcagact aagaatggta
    28681 aaggaggaaa ataaaaatga gtacaaggga attaacatat gcacaagcaa taaaggaagc
    28741 aatgtcagaa gaaatgagaa gagatgaaga tgtaatattt atgggagaag atataggaat
    28801 atatggagga gcatttggtg tatcagttgg tatgatagat gaatttggac cagaaagagt
    28861 aagggatact cctatatcag aagcagctat agcaggagcg gcagcaggtg cagcagcaac
    28921 tggcttaaga cctattatgg aagtaatgtt tatggacttt gtaacaattt ctatggatgc
    28981 aattgtaaat caagcggcaa aaatgagata tatgtttgga ggaaaagcac aagttccaat
    29041 ggttgttaga tgtccaggag gttctggaac tggttcagca gagcaacatt cacaaagttt
    29101 agaagcttgg ttttgtcatg taccaggagt aaaagtagtt gcaccttcta caccagcaga
    29161 tgctaaggga ttgttgaaag cagctataag agataataat ccagtcatat ttgttgagaa
    29221 taaattgtta tacagaaaaa aaggttttgt gccagaagat gattatgtaa ttgaaattgg
    29281 aaaggcagat attaaaagag agggtactga tgttacagta attacttatg gaagaatgtt
    29341 acaaagtgtt gaagaagcag cagagactct atcaaaagaa aatataaatg ttgaaattat
    29401 agatttaaga actttatatc cattagataa agagactata gtaaagagtg tatgtaaaac
    29461 tgggagagtt ttaatttgcc atgaagcagc taagactggt gggctaggtg gagaaatatc
    29521 agccctgata acagaaagtg aatcatttga ttatttagat gctccagtaa aaagaatttg
    29581 tggaaaagat gtgccaatac catataatcc agagttagaa aaagcagttg tcccaagagt
    29641 tgatgaaata gaagaagcaa taaaatcttt aattgttaga taaggaggga tagatatggt
    29701 ggcaaataaa ataaaagcca caccagcagc tagaagtcag gcaagaaaag acaatataaa
    29761 attagatagg ctgattggaa gtggagaaaa tggaaggatt catttagtag atgtgttaaa
    29821 ttatttaaaa gataataaag caaatactac tcctttagct agaagaatag cagaagattt
    29881 aaatatagat ttagaaacta ttgtaggtac tggatataat ggaaagataa gaaagtgtga
    29941 tgtggaaaaa ttaactggaa aagaaactat tgtatcaact aatacatcta aatctagtga
    30001 gaaaaaagag ctgaaaattg aaaatgaaaa ttcaagaatg tttaacacag tagaaggaat
    30061 atttgaaaag ccaaatccaa tgagagctac tgtagcaaaa cgaatgtcag aaagttattt
    30121 tagtgctcct gtatttacat tcaatataga agtagatgct acagaattga aggtacttag
    30181 agcaaagtta atcgacacag taaaagaatc aactggagta aaattaacta tgacagattt
    30241 aatagtaatg gcagtatcta aaatacttcc aaatcatcaa gccttaaatt cagcatggac
    30301 agatgagggt atatttagat ataaagatgt aaatatagct attgcagttg gtttagatga
    30361 aggcttatat gttccggtag taaaaaatgc aaacaaaaag tctttaaaag aaatagcaaa
    30421 agaaagcaaa gaacttgcgg aaaaagtaaa gacagggaaa cttatgccag cagaccaaga
    30481 aggtaataca tttacaataa gtaatgttgg aatgtatggt ataactacat ttacacctat
    30541 aataaatatg ccttcaagtg caatcttagg ggtaggtgct acacaagata agtttgttcc
    30601 agtaaatgga gaggctaaaa taaaacctat aatgaactta tcattaacat cagaccatag
    30661 agtaatagat ggaacagtag ctgcaaaatt cttaaaagat ttaaaagaat tgttagaaaa
    30721 tcctttatca atgcttgtat aggttttgat atttcgttac tgatataaat agtttgaata
    30781 gattatatta ttataattgt ttcagtaaat ttaaagcttt tatatattaa gaattatttt
    30841 taacagtaac tatatgtgaa tatgatttgc ttaattaaaa tatatgaaaa taaataggag
    30901 gagagcctat gtcagtagaa gtaataatgc caaaagcagg agtagccatg gaagaaggta
    30961 ctatagtatc ttggctaaag caagaaggcg aagaagtaaa gattggagaa cctatactag
    31021 aaataacaac agataaagtt aatatggaga tagagtcaga aggagaaggt acacttgctg
    31081 taatcataca taaagaggaa ggtgaagtat taccagtatt tacagtaata ggtgtaatag
    31141 cagaaaaagg ggaaaatcaa gaggaagtaa aagctaaata tttatctgga aatgtttcaa
    31201 aagaagatat agttgaagag aatcaaaata tagaatttaa agaagaaaaa ataaacaaaa
    31261 aagagtgtaa tcatgattat gatgtagtgg taataggagg aggacctggg ggatatttat
    31321 cagcattaaa agcagctctt ttaggtggaa gagttgcttt agttgaagaa aatatactag
    31381 gaggtacttg cttaaataga ggttgtatcc ctacaaagac ttatataaaa acagcagaaa
    31441 tattagaaga aattgaccaa ttatcaaaga gaggtgtaaa agtaactgta gataaagaac
    31501 aggacattaa gaaggccata aaatataaaa atagagtagt taaaaaactt acagctggtg
    31561 ttggcggatt attaaaaagt agagatgtag atgtgtttaa tcttaaagct agtgttaaag
    31621 aagagcataa agtaatttta tcagatggaa aagttttaga tacagaaaat ataataattg
    31681 ctacaggttc caaagtaagg atattaccta taaaaggaat agaatcaaat ttaataataa
    31741 ctagtacaga agcattagat ttggaaacag tgccagaaga attagtaata attggtggtg
    31801 gagtgattgg ttgtgaattt gcagagatat tcaattcaag aggttctaaa gtaactatag
    31861 tagaaatgga agatagagta atcccaagaa tggataaaga gttaagcgaa tctttaaaat
    31921 attctcttag taaaaaaggt ataaatgtat taactaaaaa gaaagtttct gaatttaaag
    31981 aagaaggaaa taacatatta gtgtgcattg aaggtgaaga gccaataaaa gcagatttat
    32041 gtctatatgc tataggaaga gaagctaatc tttctggcat agaggactta gatattaaaa
    32101 tagataaagg ttcaatagtt gtaaatagta aaatggaaac aagtatacca agcatatatg
    32161 cagtaggtga tgttactgga ggtgtaatgt tagctcatgc tgcttttaaa atgggagagg
    32221 tagcagcttc aaatgctcta ggcgtgaata aagaagttga cttaggtgca ttacctagtt
    32281 gtgtttatac aattccagaa gtagcatcag ttggtattac agaagaggat gctagaaaaa
    32341 aatataatgt aaaagttggt aagtttaatt ttgctggaaa tggaagagct ttagcttctg
    32401 gtcaagaaca aggatatgta aaagtagtag cagatgctaa atatggagaa attttaggta
    32461 tacatatgtt tggatgtggt gtagcagaac ttataaatca tgcagcatca tttaaagcgt
    32521 tagagatacc tactgatgaa gcaagtgagt tgatatttgg tcatccatgt acttcagaag
    32581 ctttaatgga agctttggca gatgtaaatg gagaatgttt acacttacct aaaaaataaa
    32641 tctaaacata aattattaat ttaagtctat tattaatatt aatttatatc ataaaatttt
    32701 aaaacactta cttagccata ccccttatta tgcaaataat ttatttgcat aataagggat
    32761 attttttatg gaaaaatata gaatatagaa tccttgtagt ttttgttgta caaaaaagat
    32821 agtaatataa aataacatta tcattttaga atgatttcaa ttgtaattag gaaaagtgat
    32881 ttttttagat taaaaaaagt attatttgta aaatataaaa aaaaatttct taatatttga
    32941 ataaaagaat agcttaattt aaatgttatt actttataaa aaaataataa gtacaaaaaa
    33001 aacaatatat ggcatataag ttgctacata aataataagg agggaacaat tataaatgag
    33061 gcaaagaata aaaaacttaa ttgaaaatga agataagaaa aatcctttaa cagatgaagc
    33121 gatttcaagt aggttaaata ttcgaagaga agatgttacc tttttgagaa atgagttaaa
    33181 aatagaagat tcaagacaaa gaagaaaagt tgttttaatt aaagctataa aagatatttt
    33241 aaaagaagaa aaaactataa ataaaaatga tataacaaga agattaaaca gcattgggtt
    33301 tacagtatca agatttacag tcatacaata cttaaaagag attcaagatt ctggtgacct
    33361 aaaacttgat aaagagagtg atgataaaaa agaagaaata atcaataata gagaaaataa
    33421 agatatagca tttgataaac tcataggtag tagtggtagc ttaagtacaa ttataaaact
    33481 agccaaagca gcaattcttt atccacctca tggtcttcat actattatta tggggccaac
    33541 tggtgttgga aagagtgagt tagcagagtg tatgtataaa tttgcagtag aatcatccag
    33601 attccctaag aatgcaccat ttatagtttt taatgcagca gattattcag agaatccaca
    33661 gcttttgcta tcacagttat ttggacacgt taaaggtgct tttacaggag ctgatgagaa
    33721 caaagaagga ttagtatcaa aggcagatgg aggaatattg tttatagatg aaatacatag
    33781 attacctcat gaaggtcaag aaatactatt tcaactgatt gataaaggta tggtcagaaa
    33841 gttaggagaa acaagactaa cacataaaat agatgtaatg attatttcag caacttctga
    33901 gccaatagat tctcatttac ttaatacatt taaaagacgt atacctgtaa caattgaaat
    33961 accagaatta gttgcaagac cattaaatga gaggtttgac attataaata atttcttctt
    34021 aatagaagct caaagaatgg gagcaaacat acatataaag tcagatgtat taaaagctct
    34081 tatgctttac gattgtatag gtaatgtagg tcaacttaga agtgatattc aagtagcttg
    34141 tgctagggga ctattaaacc aacttacaaa caaactaaaa gaagtaaata taagcatatc
    34201 agatgtacct ggatatgtaa aacaaggtct tataaaaatc agaaattgta gaggaaaaat
    34261 agaaaattat gtagatgggg atttaattat agactcaaat cttcaaggtg agatagaaaa
    34321 gaaaaatgaa gatatatata cttttccaga tgaaatatac aaacttactg aaagaagaca
    34381 tattgattta ttggaacaag gtttagattt tgatgttata aatagaatca ttggaggaga
    34441 attagaaagt tgcattcaaa aatacataaa acaagttaaa aaagtttcta ttagtaacaa
    34501 tcttccagat ataagtccaa ttgttggaaa atatatcgta gagctaactt atgaagtaat
    34561 caaaatagca tcacaatacc taagttcact agaaccgaat ctacatttat gtttagcagt
    34621 tcacctaaaa gcagcttttg agagattaaa agaaggaaaa gtaatatcaa atgctcattt
    34681 gaaagaaata ataggaaatt atccactaga atattctgta gcagaaaagg tagctcaata
    34741 tttaaaggaa agatataaca taaacttacc aaaagaagaa ataggtataa tagcaatata
    34801 ttttaaaatg agtagtagaa gagatatgtc tgaacaaaaa aagataggcg tacttcttat
    34861 ggctcacgga agagtagcat cttctgtatg tgaggttaca aataaactat taggaattaa
    34921 tcacgcaagg tatttagata tgccatttga gaagaagccg gaagaaatga ttgaagaagc
    34981 tactacaata ataaaagata ttgatgaagg tagaggtgtg attatattag tagatatggg
    35041 ttcactttca tattttggag atattatttc tggaaagact ggaattaata taaggacaat
    35101 atctagattt gatacattgc tttctataga ggttatacga agagcagttt taccagattt
    35161 aacaatagac gagatagttg atgaagttaa aggcatagag ccacaagtaa aagaaaaggt
    35221 tagtaaatgt aacaatacaa aagtagattt tagaaaacct ataattttaa cattatgtat
    35281 tactggatta gggggagctt gtaagataaa aaaaatctta gaggaaaata ttaaaagtat
    35341 aactgaaaag gttgatatag aacctatagg tatgattgaa tatccaagtc ttgatttaga
    35401 gataaaacaa ataagcaatg ataaaaatgt aatcgccata gttggaacaa ttgaccctca
    35461 gatagcaaat atcccttttt ttagtctagt agacataaat gataaagcaa agttaaatca
    35521 aataaataac ctaattgata caaatcaaat tggtgaaaca aataaaaaat atgaattatc
    35581 agatttaata cataaagaag atatatttgt ggatttacag gttaaatcaa aagaagaagt
    35641 tctaaaatat atgagtaaaa aacttagaga gaaaggttat gtgacaaaaa atttctataa
    35701 aaaagtagtg gaaagagata acttatttcc aactgaatta tcagatgaaa tagcaatacc
    35761 tcacacagat ggaatagatg taattagacc agctataagt atagccatct taaagaagcc
    35821 tattgtgtgg agtaaaagta aagtaaaaat aatactttta ttagctgtag accaaaagtg
    35881 ttttaatcca ttaagcactc tactctcatt tatagaaaca gatgaattta agaagataaa
    35941 aaacattaaa gatagagatt ttattaggga gatgatatta gatggagtca atgaaaataa
    36001 ttagtagcaa tttaattttt aaaaatattg aagtgtctaa caatgaagat gcattaaagt
    36061 ttttgggaca acgtttgttt gacgaacagt atgtaaaaga aagctacata caagctgttg
    36121 tagcaagaga aaaaaagtat gctacagggt taccaacaga aatatacgga gtggcaatac
    36181 cacatacaga tatagtacat gtaaatgagc caggaatagc aattggaata ctgaataaac
    36241 ctgtaaaatt tataatgatg ggtacagacg atactgaaat ggatgtaaaa gttgtattta
    36301 tgttagcagt gaaagagcca caagaacaat tgcaactatt agaaaaactt atgactatat
    36361 ttcaagataa aaatatacta aataatattg ttaacttaag tgaagaaagt gtgagtgatt
    36421 tattaaactc taaattgaaa aattaaacat gtaaagtata ttaaaaatgt aaaattaaac
    36481 atgcaaagta tgttaaaaaa tataaaaaga ttattaaaga tttctaacta tagatatctt
    36541 taaaataaaa taatttcaat gggggtgact aatgtggcta aaaagatatt agttgcatgt
    36601 ggaacaagag tatgtacttc aacaattgct ataaacaaat taaagaaagc acttcaagac
    36661 attggtaaat tggatatggt taatattaca caatgtaaag tagtagaagt tgcatcaaaa
    36721 gcacctgatt atgatttaat aatttgtact acacaagtat ctagtagtat aaaaacacca
    36781 gtgataaatg gattaccttt tttaactggt gtcggaatgg ataaattaat caatgatgta
    36841 ttagaagagt tagaattata attaaaaaaa ttaaggaaca tggtctctaa aatcataagt
    36901 tttagagact taaaattaaa taatggaggg tctttatatg gagatattaa atttcattgt
    36961 tggtctaggt gcacaagtaa tgatgccaat aataatatgt atttttggtt tgatattagg
    37021 tacaaaacta ggaaaatctt taagagctgg tttaactgtg ggagttgggt ttataggtct
    37081 taacactatt atagctttgc taacagacaa tttaggtcca gctacacaac aaatggttaa
    37141 gaatttaggg ctaagtttat caataataga tgtaggatgg ccagtagcat cagctatagc
    37201 ttttgcatca acagtagggg ctttaattat tccaataggt cttgtggtaa atatagttat
    37261 gctaattact aatactactc aaactgttga tgtagacata tgggattact ggcattttgc
    37321 ttttacagga gctttagtgg caggggctac tcaaagtgta atgtggggag tatttgcagc
    37381 agtagcaaat atggtaattg tattggtaat ggcagattta actgctccag gtatagagga
    37441 atatttaggt atgccaggta tatctcttcc tcatggattt acacaggcct ttgttccaat
    37501 tgctatagta gtaaataaat tattagacct aataccaggc ataaataaga ttgagataaa
    37561 tgctgatata ttgcagaaaa aatttggatt atttggagaa cctttaataa tggggtctgt
    37621 aataggtgta ataattggta ttgctgcgaa atatgatata aaaggaatat tacaattagg
    37681 tgtaactatg ggagctgtat taatattaat tccaaagatg gcagcattat taatggaagg
    37741 attattacca gtatcagaag ctgcacagga atttattgag aagagattta aaaacagagg
    37801 caagatttat ataggcttag attcagcagt aggaataggt catcctgtta cattatcagt
    37861 tgcactagtg ttggttcctt tgactatact tatagcagct atattgccag gaaataaagt
    37921 tttaccattt gcagatttag cagtaattcc atttgcactt gtattaatcg taccaattac
    37981 aaagggaaat gtgtttagaa cattaataat aggaataatt ataattactt ctggattatt
    38041 aatcgcaact aacttagctc cattatttac ccaaatggct ttaaatgcta gttttaaaat
    38101 gcctgaagga gcaactatga tttcaagtat ttgtgatggt gcgaatccat taagttgggt
    38161 atttgtaaaa gttatgaact ataaagttat tggaggagta atttttggag ttatagcttt
    38221 aggaatggct atttacaata ggaatagaat aataaatgaa aataaaaagc tatctataga
    38281 agaataaggt taaaacaatt ttattaagga gtgtataggt tatgaaaaaa tataaatatg
    38341 attctaaaag gtacttactc agtgttacaa taccaggatt ttttatgatt ttaatattga
    38401 tttatgcact gtttaataac tatacaaatt ttaatttgaa tatatatagt ttgttaataa
    38461 ttgtctgtac ttatggtata ttcaatactt ttatatcatc aagtaatcct aaaaaaatta
    38521 ttattgatag caaatgtata cattttacct catttatgac tacacataag tatgaaatta
    38581 aagatataga aaagataaga ataagagaat tttataataa aaaaatttat ctaaaaatta
    38641 ataatggaaa cctttttaaa ggtaggtatt ggataagaac aaatatgttt aataatagta
    38701 tagcattata tagctatttc attgagttgg aagagtgctt atatccagat tcactaaaat
    38761 ttagaaataa aaaatttgag aataagaaca tataaaaata aaaaattgaa ttgaagccta
    38821 gcttaatctt ttttgataaa atataagttt gaactttata ctaaattttt agaattttca
    38881 atcaagacaa taacataata gctaagcaat tagaacacaa ttaagatgta taaggcatgt
    38941 atatgatgaa tgggaggatt taattaatgt tattagagaa attaagaaaa gaagtattac
    39001 aagcatcttt agacctttta aactataatt tagtgacatt aacaggagga aatgtaagtg
    39061 ggagagatga acaaactggc tatatagcaa tcaccccaag tggaatggat tatagaaatc
    39121 ttacaccaag tgatattgtt atagtagatg tagatggaaa tattatagat ggaaaatgga
    39181 aagcatcagt ggatttatca gaccacttgt atatatataa gcatagagaa gacataaata
    39241 gtataattca tacacattct acatactcaa gttgttttgc tattttaaat gaacctatag
    39301 aatgctcaag tacaactctt gcaaatgaag tagggggttc agtaccagtt gcaaaatttt
    39361 caccaccaac atctaaaaag atgggaaaat gtgttattga agcaattggt gacaaaagag
    39421 catgtctttt agcaaatcat ggcgtaattg cagttggtcc atctgtagga catgctctta
    39481 cagccgctgt aatgttagaa gattcagcaa aagtatatta cttagcaaaa tcaattggca
    39541 caccagtgct actgcctgat gaagaaattc aaagagcgag agatgtattt tttaatgtct
    39601 atggtcagga taaataaatt agataaaatt ttaaaagtaa tatgaaaata tagatattca
    39661 atgatattaa ggagatttat aatgatataa taagtttaat agttaagaag taaaatatga
    39721 aatgtaatag tatataaatt catttaagac taaaaataat aagataaaat aaaaaggagt
    39781 ctgtatttct atgtattact tattaaacaa aagcacaaat ccttacttta atttggcatt
    39841 agaagaatat ctatttttaa atgataaata taatgatgat attataatca tatggagaaa
    39901 tgaagaatcc atttttatag gtaaaaatca aaatccatat caggaagtat atcatgatgt
    39961 tattgaaaag ggagaaattc ctattttaag aagaatatct ggaggaggaa ctgtttatca
    40021 tgacttaggt aatatcaata tgtcattcat acaaaaagat aggcaacttc atgagataga
    40081 ttttttggag catactaagt ttatgcaaaa gatgctttca accttaggac tagatgtatc
    40141 tataactgaa agaaaagatt tatttttaaa tgggaaaaaa atttcaggtt ctgcacagtc
    40201 tataaaaaga aaaaattctc tttatcatgg aactcttctt tacgattcag atttaaataa
    40261 attaactaaa tatttaaatt caaataaagc aactgaatca aatgcaacta aatctgtttc
    40321 aagtaaagtt acaaatatta agtttttact cgaaaaagat ataaattact ttttagatta
    40381 ttgtgttgaa tatttaaaat ataatataaa gaatataaaa gaacttgaat tggatgaaga
    40441 agatattaaa agtgtataca atataggaga agataaatat aaaaagatag agtggacata
    40501 tggtaaaacg ccgaaatttc aagtagtgct accatttgat aatgaaagta aaataaatat
    40561 acatgttaac agatggaaag tatcaaaatt ttttatttct tatgatgata atgtaattaa
    40621 tatggacaag ttgttagatt tgaatttctt taaagaagaa ataaaagaga gtattttaga
    40681 aaattatcca cattacatag atttaatcaa tttaatattt taatataaaa cctctaattt
    40741 gataaaattg tcttatatca aactagaggt ttttaatatt cttgcatatt aacaaccatt
    40801 a
//
LOCUS       DAEMIK010000016        40453 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEMIK010000016 DAEMIK010000000
VERSION     DAEMIK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.016"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(63..1268)
                     /locus_tag="KPK87_000727"
     CDS             complement(63..1268)
                     /locus_tag="KPK87_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG2536902.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1443)
                     /locus_tag="KPK87_000728"
     CDS             complement(1291..1443)
                     /locus_tag="KPK87_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2536903.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            complement(1446..2831)
                     /locus_tag="KPK87_000729"
     CDS             complement(1446..2831)
                     /locus_tag="KPK87_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG2536904.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2860..3246)
                     /locus_tag="KPK87_000730"
     CDS             complement(2860..3246)
                     /locus_tag="KPK87_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2536905.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3262..3576)
                     /locus_tag="KPK87_000731"
     CDS             complement(3262..3576)
                     /locus_tag="KPK87_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2536906.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            4103..5029
                     /locus_tag="KPK87_000732"
     CDS             4103..5029
                     /locus_tag="KPK87_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2536907.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTENSTYKNPESIHEII
                     IRKLNNESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(5382..7034)
                     /locus_tag="KPK87_000733"
     CDS             complement(5382..7034)
                     /locus_tag="KPK87_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG2536908.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            7633..8754
                     /locus_tag="KPK87_000734"
     CDS             7633..8754
                     /locus_tag="KPK87_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2536909.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            8790..9203
                     /locus_tag="KPK87_000735"
     CDS             8790..9203
                     /locus_tag="KPK87_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2536910.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            9196..9735
                     /locus_tag="KPK87_000736"
     CDS             9196..9735
                     /locus_tag="KPK87_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2536911.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(9935..10420)
                     /locus_tag="KPK87_000737"
     CDS             complement(9935..10420)
                     /locus_tag="KPK87_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536912.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            11325..11702
                     /locus_tag="KPK87_000738"
     CDS             11325..11702
                     /locus_tag="KPK87_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2536913.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            12098..13903
                     /locus_tag="KPK87_000739"
     CDS             12098..13903
                     /locus_tag="KPK87_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2536914.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            13980..14519
                     /locus_tag="KPK87_000740"
     CDS             13980..14519
                     /locus_tag="KPK87_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBG2536915.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            15178..16392
                     /gene="dpaL"
                     /locus_tag="KPK87_000741"
     CDS             15178..16392
                     /gene="dpaL"
                     /locus_tag="KPK87_000741"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG2536916.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            16759..17946
                     /locus_tag="KPK87_000742"
     CDS             16759..17946
                     /locus_tag="KPK87_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG2536917.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            17962..19545
                     /locus_tag="KPK87_000743"
     CDS             17962..19545
                     /locus_tag="KPK87_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG2536918.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCNVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            19573..20901
                     /gene="ssnA"
                     /locus_tag="KPK87_000744"
     CDS             19573..20901
                     /gene="ssnA"
                     /locus_tag="KPK87_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG2536919.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            21046..22398
                     /locus_tag="KPK87_000745"
     CDS             21046..22398
                     /locus_tag="KPK87_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG2536920.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     TAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            22590..23699
                     /locus_tag="KPK87_000746"
     CDS             22590..23699
                     /locus_tag="KPK87_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2536921.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            23982..25373
                     /gene="hydA"
                     /locus_tag="KPK87_000747"
     CDS             23982..25373
                     /gene="hydA"
                     /locus_tag="KPK87_000747"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG2536922.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            25367..27928
                     /gene="xdh"
                     /locus_tag="KPK87_000748"
     CDS             25367..27928
                     /gene="xdh"
                     /locus_tag="KPK87_000748"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG2536923.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            28080..29426
                     /gene="rpoN"
                     /locus_tag="KPK87_000749"
     CDS             28080..29426
                     /gene="rpoN"
                     /locus_tag="KPK87_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG2536924.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            30208..31230
                     /locus_tag="KPK87_000750"
     CDS             30208..31230
                     /locus_tag="KPK87_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2536925.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            31290..32297
                     /gene="gap"
                     /locus_tag="KPK87_000751"
     CDS             31290..32297
                     /gene="gap"
                     /locus_tag="KPK87_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG2536926.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            32519..33721
                     /locus_tag="KPK87_000752"
     CDS             32519..33721
                     /locus_tag="KPK87_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG2536927.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            33747..34490
                     /locus_tag="KPK87_000753"
     CDS             33747..34490
                     /locus_tag="KPK87_000753"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG2536928.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTDIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            34499..36031
                     /locus_tag="KPK87_000754"
     CDS             34499..36031
                     /locus_tag="KPK87_000754"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG2536929.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            36619..37911
                     /gene="eno"
                     /locus_tag="KPK87_000755"
     CDS             36619..37911
                     /gene="eno"
                     /locus_tag="KPK87_000755"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG2536930.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            38516..38740
                     /gene="secG"
                     /locus_tag="KPK87_000756"
     CDS             38516..38740
                     /gene="secG"
                     /locus_tag="KPK87_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG2536931.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(39124..40062)
                     /locus_tag="KPK87_000757"
     CDS             complement(39124..40062)
                     /locus_tag="KPK87_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG2536932.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSILTVFLIIFILKQNNLI"
     gene            complement(40050..40448)
                     /locus_tag="KPK87_000758"
     CDS             complement(40050..40448)
                     /locus_tag="KPK87_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2536933.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
BASE COUNT        15504 a         4972 c         7415 g        12562 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacg gggggttaag tgctggcgtg gctattgcca caccagccag
     1441 caagatcagt ccacacctgc gacttccgct tcgcacgtcg cctgcgtgga ctgtctgctg
     1501 ttggataact ttttaatttc ctccaagaaa tcatatcctt ttggtacaag gggagtataa
     1561 aactctgata tgacacttgt tcctacatca acatagccac gacctttgat tcgctttaag
     1621 aagaaatcct tttgtacgtc actgccaaac atcatgccat agcccatttc agacatacga
     1681 cctaaagcca ctctgaaatt aaactgatca cggattccgt cgcctaaata ttttgcgtct
     1741 ggacgttgac aagccagtat tagaaagaag ccagcttgac gacctaacat gacaatctgt
     1801 ttcagcttat tcataactgc ggtgttttct tttgttccca gcatttccat gaaagcgacg
     1861 tattcatcaa agattaagaa gtgtgccggg agacctaagt aagcataatt tttgccagtc
     1921 ttatagttct tcatctgctt catttcctca ctacgtttca tcatttcttc atagaatgtt
     1981 tcaatgcaag aaagcaagtc ttcttttcta tagtagacat ttgccatcac agaacctaag
     2041 tccgcaagat cagcattttt cgggtcaaga atatacagtt ttgaatctgt atgaagcaag
     2101 gcttcaatca gtgtcagtat aaagtaagtt ttaccgccac ctgtaccacc agcaatcaac
     2161 atatgaggga gcttatcata ttcccaccat acgtttttca ttaagcgaag tttaccatct
     2221 ttagcttcta cttcatcaat agaaatacga ctggctatgg tgtcatagag caaagtatat
     2281 tccacatagg aatcctttaa ctctttatcc gtcagctcac agtacaagcc actctctaat
     2341 ttcttttcca agtgtaagag ttggtcttga tattttccca gcgtgatttc cacccgtatc
     2401 tgtatcaagc catttttaag tcgataatac attttaggga agtaggttat cttttccttt
     2461 gtacgaccag cactatcttt aaagaaaccc tctgttttga cctgttcaga ttcataccac
     2521 ttgttttcaa gtatcatctt tgccagtttt tgacggtggt aaagttgttt aaccgtatca
     2581 tagcgaaccc gtttgaatac aaacgctacc agcaagcaga taagaattgc gacactgaaa
     2641 ctgataatta aatagggaat gtcaatctta tctgcttgtg ataggttaaa atcctgccag
     2701 ttgatctgct ggattgtctt cacatgaaac agtccgacaa ccagcaggaa aacaggcagg
     2761 agtgacgcta tcgtaaaatg aaagactaaa tctttaccag atgggcgaat ccttttacca
     2821 cgctgtttca tgcgaaaaag tctcctttct acctagcgac tatttgtctt gtgtcggttc
     2881 tttctttgct tgtggttgag ctttgaatga actagaatcc tttgtcagca caatatcgtc
     2941 tgccttgata taccagtcaa catctgctcc ttgataggtg gcagtagcaa cggtgtccgc
     3001 aatgggattg ataagttcca cccgtgcgtt ataatcaaac tctttcaaag gcacgctggc
     3061 aggaatactt acttgaatca tgcgtccttg tcctttggat tttaagtcat aggtacgttc
     3121 cttgatttca tctgaaaccg acccgtcttc attttggatt ctcacttcac gacgtagagc
     3181 agagaatttc aattctccaa aagtcgtgtc tttatctaat acaatgccat ttgctaatct
     3241 catcattttt cctctctttc tttattcttt tatcatgtcg tcagcatgta aaaggtaatt
     3301 tgtaaaacca cgagtgccga ttttgtagcc ctctgcggta atacgtggat tgactaactt
     3361 cacacgttcc tcaaagccga aatgtttttc gccagcttca gcaggaagca ccaccacaat
     3421 atcatctgct ctttgaacat cagaatagag attatagctt cttgataaga cagttagccg
     3481 tccgttgatt cttcgctgaa cgactttatc ctcgccagca aattctaaat tgccgaatgt
     3541 tttttccatg ttgggaatca caaatttaag ttccatattt ttacctatcc tttctttttt
     3601 attggctgaa tgaatgtttg atggtcttaa agagtgggga acgacctttt gattcttgat
     3661 tttttgtttt cataagttca cttcctttca aaatcgggta aaaaaataga cacctcattt
     3721 tttgaagtgt ctacctatta aatattcaaa ttttattgga agtatcttta tatcttcact
     3781 tttcaaggat aaatcgtcgt atcaaagctc attcataagt agtaaattag tagtaaattg
     3841 agtggttttg accttgataa agtgtgataa gtccagtttt tatgcggata actagatttt
     3901 tatgctattt ttagtataaa aataatatta agtttattgt ttaaataaaa aagttaaatc
     3961 tacactatta acttattata cttaaaactt taaaatgaaa atatcaaaga atacttttta
     4021 ataaaaataa tcatttaata agttatttaa aaatctaaaa actttacaac aaattatcta
     4081 tttaaattgg aggagtggga atatgagtaa tagtttttat caattaaatt cagcagtatt
     4141 atattttaat gaaaaaagag atgtaaatag aataaggttt aatcactatg taatgagaaa
     4201 agaaaatata caaagattaa aaggaactct gccaagagga caatttatga tgacagtaag
     4261 gaaagctgca tctgaccttg atgtatctat atctacaata agtagacttg taaatgaatt
     4321 tatagattta ggtatattga gattaataag taggggagta aagggaaatt gttgttctgt
     4381 atatagttat gtttgttctg aaagtagtaa tgcagaaatt gtaacaaaga atggtgatat
     4441 tttcaaaaat atagagaaat acattgatgc aactattaac aataaccaaa aaacaatgaa
     4501 atataaacat agagatgagt tgtttaagaa cagtaatacc aacatataca gccgtataaa
     4561 tcaagagagt atagacaaag attatgattt gataattcat actgggtatg aaacagaaaa
     4621 tgagacaaag aaaaaagaat tattaaaaaa taatttaaat aaaaagaatt taaataaaaa
     4681 tacctttgaa aattcggatg aagatttgac tgaaaattct acttataaaa atccagaaag
     4741 catacatgaa attattataa gaaaattaaa taatgaatca ggaaaaaatt ttaaagctga
     4801 tttgagtata actaaggcac ttatagataa aagattaaaa gaagggtata gtttagaaga
     4861 tttttacaag gttatagagg caaaggtaag aagatggaga tatacagata tggaaatata
     4921 tatagaacca acaattctgt ttgggtatga atttgagtcg tatttaaatg aggagagttc
     4981 tttatctgct tgtaattatg gtgatatgtg ttatgaagat atgtattgag atattagttg
     5041 ctttttagtt gtttaaaata tgtttatata tgaatataaa tagtgtatta aaatggagtt
     5101 gtctcaaaat agagagaagc ctcttcttaa ttgtttaaat gatgaaaata tatttgattt
     5161 aatacaaagg agagtgcctt atgagctaga aaattcattt tgagacactc tcttttttta
     5221 atatactgga gaaatataac ccattttaca agagtgcata gatatatata atggcataat
     5281 gtggatttaa attaaaaaaa taaaataaag ccataaatat tgaaaaattc aatatttatg
     5341 gctttattgc ttgtaataat taatagaata tatatttttt attaatcatc tatttcaaat
     5401 tctaatattt taccagtaat tgcattaatt gttatctcat attcagtttt tccttttatt
     5461 atttcacctt catattcttt gtcatcttca tcatacttaa attttactat tttcccacca
     5521 ggcactttat ctagcattat tgatttagct ttttcagcac taataataga actgttgtca
     5581 ttagtattag agttattctt atcattatca tcaaagtcat catcagaatc attgtccatg
     5641 tcaatatcta caattcttcc atcaaatgca cttatctcta attcatatgt tttcttattt
     5701 tttattactt tagcctcata ttcgttatct tcagcatcat atttacaata gattaaatta
     5761 ccattaggaa tagcatctaa tgctatagac ttagctttat ccaatgatat attttgtgta
     5821 cctaaattat tatcatttaa gtcatcatct ttttcaattt ctattatttt accagagtag
     5881 gcatcaattt ctatctcata taggatatta tcctttataa ctttgcagtc atattcatta
     5941 tcatcttcat cataataaca aactaaaata ttgcttcctg gaagagcttg ttttaatatt
     6001 tctttagcct gtattaaaga tattttttta gttttatttc ttatgttcac attttttagt
     6061 tttaaaatat cttcaaatgc tttttgatta tatcctcctc ctacttgtgt tatttcatct
     6121 atatctttat ttttaataaa ttctttttga ctatttacaa ggctaccatt agatatcatt
     6181 ataggagaat tttctttagc agctagtact cctattgata atgaatctac taatgcattt
     6241 tcttggttaa taccatcttt agctacaaat acattattta tttttttatc tttataaaat
     6301 ttatcaataa tttttgcatt agtattgttt ctatctgaac cactgattct ttctctgttt
     6361 ggatatttct ttactgtatt gtctgataaa atcttatttc ctccaactat gtaagatttt
     6421 tttatagacc tattgtttaa gaaatttata ctactatcat tgctgttttc atctgatact
     6481 aatattatag gcatattatg tattgctgct ggagaagcta tactaactgc atcagatagt
     6541 ccctttagtc catttacaac tgcaacattt tctatctttt ttatcttatc tatttcttta
     6601 agtacatttg tagacatttc atatatattg tttcctacta ttttatttat ttttatgtta
     6661 agtgatttta tttcattttc tactttagaa gaaatgctat ttccactatc tacaatgtac
     6721 acattatcta caccaagcct tttaagctca gccttagttg aattttcaat actgttgttg
     6781 tttactaaaa gtataggtgc attctttagc ttagcaaatg gatttgcaca aagcgcactt
     6841 tgaatagaat taccattaac tataatggct gtttttgatt gaacccatcc caatttactt
     6901 acttctactg ctgtttcata tcttgtttta cctattaatc tttcttgctt tgtagtattg
     6961 tctgcaaaaa ttgctgaaga actagaaaaa aacatcatac ctgatattaa tatagctaaa
     7021 tttttcttat tcatattttt acctcacaat ctttaaaaat ttgttattta gcatacttaa
     7081 taaaatataa tcttaaaatc aaattgatac tgagtttgaa gttacaaaat caacaaataa
     7141 gatttcttta atacaaaatt ttccttttta tatttaacat tgtatcacaa atataaaaag
     7201 gaaaaaactt tttaaaaagg gtatacacta ttcatttttt atcttataaa taagtcttta
     7261 ccactgtttt atatatttta aaatagacac aagacctatt tctaaatctt ttataaacat
     7321 tttaggtgaa atttcttttt aaacatatct aaaaagtatt tgtagtaata ttaatggact
     7381 gaatttctta aagtatatat ttctttacat gttggagttt gtgaagtttg agaattggag
     7441 ggtgaaagaa acatgtaatg gattaatatt aaagaactgt atttttataa tatttgtctt
     7501 aaaaataaaa aaatctattg acttgaagtt aactccaagt agtaaagtat aattatacaa
     7561 gaattatata cttgtaagaa tagttaaagt agagagaaaa aacaaacaag ttttaaaggg
     7621 ggaagtaaaa caatggaggc aaaaaaatta ggatttggat taatgcgttt accaataaaa
     7681 gatgaaaatg atgttacaac tattgatatg gaacatttaa ataaaatggt agacactttt
     7741 ttagaaaggg gatttacata ttttgataca gcctatgtgt atcacatggg aaaaagtgag
     7801 atagcactta gagaatctct agtaaaaaga cacaaaagag agagttttac tatagctaca
     7861 aaacttccat taatggcttt aaagaaaaaa gaagagcaag aaaccatttt taatgagcaa
     7921 ttagaaaaat gtggtgtaga ctattttgac tactatcttt tacacaatat aggcgtgtca
     7981 cattatgaag tagctaaaaa atttgatagc tttaagttta ttgaagaaaa gaaaaaagaa
     8041 ggaaaaataa agaatattgg tttttctttt catgatagtg cagaattatt ggataaagtc
     8101 ttgagtgagc atccagaagt agacttcgtg caattacaga ttaattattt agattgggat
     8161 aatgaaagca tacaatctag aaaatgctac gaggtggcag agaagcataa taagcctgta
     8221 attgttatgg agccagtaaa aggaggtaca cttgcaaaga taccagaaaa agcagagaaa
     8281 ttattgaatg actacaatcc agatatgtca atttcatctt gggcaatacg ttttgcagca
     8341 agtaaagata atgttatgat ggtcttaagt gggatgtcaa atatggaaca attattagac
     8401 aatacaggat atatgcaaaa tttcaaacca ttcgtacaag aggaatataa tattatagat
     8461 gaagcagtag agattatcaa cgaatcaatt ttagttcctt gtactgcctg tcagtattgt
     8521 gtagaaggct gtccaaaaaa tatcgctatt ccaaactatt ttgcgttgta caatgcagaa
     8581 aaacaagctc ttaatactgg attttcaaca cagatggtct actataataa ttatactaaa
     8641 acttatggaa aggcctcaga ttgtattgaa tgtaaacaat gtgaagaaag ctgtcctcaa
     8701 cacattaaaa ttatagatgc tttaaagaat gtagcagaaa cctttgagaa ataattcaat
     8761 ctatacaata tcaagaggag aagatatgta tgacgatttc agaagttagt aaaaaatacg
     8821 agctttcagc ggatacttta cgatattatg aacgaattgg tttaatacca cctgtaaata
     8881 gaaataaaag tggaattagg tcttttacag aagaagattg tgagtgggtt aatttcataa
     8941 agtgtatgag aggtgcaggt ctttctattg agacattaat tgagtatgta gctatgtttc
     9001 aacaagggag cagtactatc aaagctagaa aagaactttt aatagagcag agaaaccaac
     9061 tagcaaaaag aatagaagaa atgcaaaaaa ctttggaacg attaaatttt aagattgata
     9121 gatatgaaga aggtattatt gagaaagaaa aagtattaaa gagcagtaga aataagaaaa
     9181 tggaggcagt atcttatgag taaaaatgta ttaatagtat cagctagtcc tagaaaaggt
     9241 ggaaattctg atttattgtg tgaccagttt atacaagggg tattagaatc taaaaatcaa
     9301 gtggagaaga tatttttaaa agataaagat attaactatt gtactggatg tggtgcttgt
     9361 tatgaaaaag aagcaaattg ttcacagaca gatgacatgg gagaaattct tgaaaaaatg
     9421 attgaggcag atgtgattgt tttagcgact ccagtttatt tttatactat gaatgcgcaa
     9481 atgaaaacta tgattgatag aacttgctct agatatactg aaatttgtga taaagatttc
     9541 tattttatcg ttacagcagc agatgaaaat gtggctgata tggaaagaac aatagaagga
     9601 tttagaggat ttacatcatg tctaaatggt tctaaggaaa aaggtatagt atatggtgta
     9661 ggtgcttgga atgtaggtga tattaaggga agtaaggcaa tgaaacaggc atatgaaatg
     9721 ggtaagttgg cgtagtatgt ttgtataaaa ttgtataaaa ttgaatatat tcttatcatt
     9781 tcaacaataa aaagaggctt atctgtattt tgagacagcc tctttgtgct atatcattta
     9841 tttttattct atatccttat tatacccttt atttttttat gcctatttaa gtatctgttg
     9901 ttgctcatat attttaataa ttacccttaa aaaactatac agattcctgc ataatattat
     9961 tttctttagt aattaatttt tctaggtttt tccaatccat ttcttccata tttgcaaaat
    10021 aatgaccact tcctatgcaa gtcaggtaag actttccatc taattcaaac gaagaacctc
    10081 caatacactt tgaattacat ttcttacaag tacgagtttt agttatacta tctttaatag
    10141 ttttagagca ttcctccaca gcatctttat atgtatttag atgaaaaaga tttgctttaa
    10201 ctctccattt tttttcatta cattcaatag taaataagct acgttttggt ttcttagttg
    10261 aatatactat tttgtaagaa cgagcacctg tttttggttc aattttacaa tctaatgatt
    10321 taccaagttc atgtaaatga catgcaatat cataatcttt accacttagt acttctaaca
    10381 tttcttctgt tgtttctgct gttaattcac ttttgctcat attaaaaaaa tccccctata
    10441 taaataataa tttcatatga aaataattaa tataagtata ttaaataaaa ctattaaaat
    10501 caataaaatt actgcaatct tacattattg ggcatagaat gagagctaat gatttattag
    10561 aaataagact taatagtatg aaaatttact tagacgtggt acaattctat aaaagattta
    10621 aaactaggaa aggagaaagt tgttgagata cggccaatat aacaatttat ttataagttg
    10681 taaatttatt atgagattaa ttagattgtt aagtaatact atcaaaaggt agttttttgg
    10741 attttcagca acagtggtag atattgataa ttttttggtt attattatat ttagctgtta
    10801 tattttcaat aggctctatg ataactaggg taaataaggt atgggtaaag gttttattat
    10861 ttataatagt gggattgtca actatagttc tttcatatgt aataacttga ttatgcaaaa
    10921 ttctatgaag caggaaccct gtgactttag tcatgggagg ttcagaattt aaggttaaaa
    10981 aataacttta atctgtaaat tctcaaaaca agaattctca tgttggtaaa tttctagata
    11041 tgagaaaaaa attgacatca taaaaaagta attttagagc gtttttttac gaaaaaattc
    11101 agaagtaact attttgaatg acttcacaaa attttatata taaataaagt ttggcacgtt
    11161 aattgcttta ataaaagtta acaagacaca aataaacata atcatctaag tattgattat
    11221 ttgaagtttt agatttgaaa tttagtctaa aggttcgtat taatattggt gtttaggaaa
    11281 ttttattttc ctgtaaatat aacttaataa taggaggtga agtcatgtca aaagtagtaa
    11341 tagctacaga gaaagctcca ggagcaattg gaccatattc tcaagcaatt caagttggaa
    11401 acatagtttc tacatcaggt caacttccaa tagatatgaa gactggcgag ttagtatctg
    11461 acccaaaaga agcaacaaaa gcttctttag ataatgtaaa agcaatatta gaacaagctg
    11521 gtacaagtat ggataaaata tttaagacta cagtattcgt aaaagattta aaggatttta
    11581 atgatattaa tgaagtatat gctacatatt ttaaagaaaa tccaccagca aggtcatgcg
    11641 tacaagttgc tcaattacca aaagatgcag ttttagaaat tgaagcaatg gcagtattat
    11701 aagtattata acaataaaac caataacaaa actaataaaa attaaaaact aataaaaaaa
    11761 gtctacaatt aaaaaagttt atattaaaaa agtctatata aaaatctatc taaatattta
    11821 actattgagc ttttctcata taatgtatat ttttatgtca aaaatatatt tgttatatga
    11881 ggaaagcttt tttttattat tgtattgtga tttattttat tgtctgcata ttataattaa
    11941 ggttactata taaaacaaac tttttataaa ttaatattgt ctgttttaac gcaaatatat
    12001 aaataagtaa tgttttatat atattttaaa gagtgactat atgttaaaat atagctaaac
    12061 tgataatttg gtaaattaaa ttctaatggg ggaaactatg ttaagtaaat taaaagaatt
    12121 tcaacaagaa atgataaaat acactgaaac agtagctagt gtgttagatg tagatattga
    12181 aatagttgac gatagattaa taagaattag tggtacaggt ctgtataaat caaagataaa
    12241 tgaaagcgtt gttacagaag gatttattta tgacaatgtt atacagacag gtcaggaatt
    12301 agtcgtattg gatatatgtg ataatcagct ttgtatagaa tgttcccatt acatgaaatg
    12361 cctaaataaa gtaataatag cagttccaat aaagtataat aatcgaacta taggagtaat
    12421 tggtgctatt agtacagata aaactaaaaa agtagaaata agtgcaaaaa tagataatta
    12481 tttaaaattt gtcaatcata tatgcgattt aatttctatg aaaatagaag aacatgaagc
    12541 gtcaaaaaat agcagtagaa aaatggatat gatgatagaa atcattgaaa atgttgaaaa
    12601 aggcgttata atactagaca taaatagcaa aatatcttat ataaacaata tagctctaaa
    12661 aaaattagat atagataaaa atataataga aaatatagta aatatagtat ctgttgaatc
    12721 ttcctcaaat ggacatgaat tactagaaat tgatattgat aataaaattt ataatataaa
    12781 tgccaaaata attccggtat atccatatat aaatcaatac gacaaaataa taatatttga
    12841 caagacatat atcaatcata aaggacatgg aaaagtgaat agtggctggg gaaatagtga
    12901 tatagagtct ataattggaa attcagaagc aatgttaaaa gtaaaagaga gaactaagaa
    12961 attagcaaag tcaaattcaa ctgtacttat aactggggaa agtggtacgg gtaaagagct
    13021 aattgctagg gctatacatg ctgaaggtag cagatggaac aagccattta ttgcgataaa
    13081 ctgtgctgct attccagaaa atctccttga aagtgagcta tttggctata taaaaggtgc
    13141 atttagtgga gctagtagtg gaggtaaagt aggtaaattt gaacttgcaa atgaaggtgt
    13201 tatatttctt gatgaaatag gagatttgtc aatgccttta caagcaaagt tacttagagt
    13261 tttacaagag agaaaatttg cgagaattgg gtcaaataaa cttatagatt tagatataag
    13321 agtgattgct gctacaaata aaaacttatt gaaattagta aatgaaggta agtttaggga
    13381 tgatttatac tatagattga atgtaattcc tataaatttg ccacctctta gggaaagaaa
    13441 ggatgatatt gaggcaataa tgatgaagtt tgcttctaaa tattctttag aattaggtat
    13501 tcagttaaat aaaatagaag aaaatgtaat gaatatgttg attaactata attggccggg
    13561 aaatataagg gaactggaaa atgcagttga atatatgatg aatttggttg gagatgatgg
    13621 aataatatat aaagatatgc ttccattaga tattttaaac tattataata ttaatggaaa
    13681 tatttataaa aataaggatg taaatataat atttgaagat gatatagttg gagggatagt
    13741 agagaatcaa gaaaggatac tttcaattaa agaattagag ttgacttaca taaacaagtt
    13801 gttgaataag tatggaagag atacaaagac gaagaagaag atagcaaaag acttaggcat
    13861 aggtttggct acactatata gaaaattgga agaggaacag tagtaatata atattaaaat
    13921 gataggtatt tgtgtgtttt tatagatagg tatttttact ttatggtgga ggtgttatta
    13981 tgcaatatgc gatagagcta tattatgata aaaaaacgga aaagcagctt tttgatttat
    14041 ctaaaaaaat cgctgatgaa aaaattagta cgaagtactt agaatggaaa acaagacctc
    14101 atcttacttt ggcttgtttc aatgatgtgg atgaggcttg ttgcatagac aagctaaagg
    14161 ggtttgcaca aaaccataag gtaatacctg cttacattgg ttcagtaggc atgtttaatg
    14221 atactaaaac ggtgtttgta tcacctataa tgaatagcag tatgtatcag tttcagagag
    14281 aattatatga ggtactacaa gattttgatt caaatggttg ggaatggtac caccccaatc
    14341 gttgggtacc ccattgcaca atagcactta ctggagatga tgaaagtgag gctttttttc
    14401 gggcaagtga tgtattactg cgtgagttca aaaaaataag tggtgagttt gtctcaatag
    14461 gattggttaa aattacattt cctgtggaag aaatttttac agtgaatatg aataggtaac
    14521 tttaactctg ttgtggatgt tatgaatatt ttgtatagtg catttcatca gataaatgcg
    14581 aagttgcgaa tttcgacagc tcaatagtcg caagtagggc acaatggtac acatattgca
    14641 cttttgctta ttggggggac gaccatgtaa acttctgtaa gaagctatga aaagatttaa
    14701 gccattttgg aggctttggg taataaagaa tatttgggta ataaagaata tttgggtaat
    14761 aaagaatatt tgggtaataa agaatataaa gtatataaat ttatagagtt tatgcgtaag
    14821 aaggttatag ccatatattt atagaaaata aaagtagtaa tatatctgac aaaacaatag
    14881 acatataaaa aataagagca taataaagga agaattaaga taaataaacg atttctcgaa
    14941 ttgataattc tcgtattgat aaaaatttcc caatatgaga attttttctt ttataaatct
    15001 tatttttcac actcaaaatt tataaacttt aaagaaattt tataaaagta tgtaattcca
    15061 acataaaaaa attgttttta gaataaataa tattggcacg gtaattgctt tgctataagg
    15121 cacaaagata aaaaattaaa atgacaccga aaaatttgaa aggggaatat aaaataaatg
    15181 agagaaataa aatggaaatt aaataactta cctaaatcag aggaaaaaga aaaaggaatt
    15241 gaatttttaa aagatgaaga gattgcaaag gctaaagctt ttcatgagag ctttccacaa
    15301 tatgaaaaga ctcctttagt taaattagat aacttagcaa aatcactagg cgtaagcgga
    15361 atatatgtta aagacgaatc ttatagattt ggtttaaatg catttaaagt tttaggtggt
    15421 tcttactcta tgggtagata cttagctcaa agattagata cagatatatc tgagttaggg
    15481 tatgataaat taacttcaaa agaaataaaa gaaaaattag gagaaataac tttctttaca
    15541 gctacagatg gaaatcatgg tagaggagtt gcttggacag ctaataaatt aggtcaaaaa
    15601 tctgttgtat taatgccaaa aggttcttct gaatttagat taaataaaat caaaggagaa
    15661 ggtgcagatg ctagtataac agacttaaac tatgatgatg cagtaagatt agctaatgat
    15721 tatgcagaag ctgatgacca tggagtaatg gttcaagata cagcatggga cggatatgaa
    15781 gaaataccag catggataat gcaaggatat ggaactatgg ctaaagaagc tatagaacaa
    15841 ttaaaagaat atggagtaga tagaccaaca cacgtatttg ttcaagcagg agttggttca
    15901 cttgctggtg cagttcaagg gtatgttgct tcaatatatg atgaatgccc aataacagta
    15961 gtagtagagg ctgatgaggc tgattgttac tataaatcag cagaagctgg agatggaaaa
    16021 ccaagatttg ttggaggaga tatgccaact ataatggcag ggcttgcttg tggagaacca
    16081 aacactatag gttttgaagt gttaaaaaat tattcatcag cattcgtttc agcaccagac
    16141 tgggtatctg ctaaaggtat gagaatatta ggaaaccctc taagaggaga tgagaaagta
    16201 atctctggag aatctggagc agttacaact ggagcattag tttcaatcct tgaaagtgaa
    16261 gatttaaaag acttaagaga agctttgaaa ctagatgaga actctaaagt attattaata
    16321 agtacagaag gagatactga ccctgataaa tatagagata tagtttggaa tggtgaatgc
    16381 caaagtaaat aaaagactta aggaaaagaa aaacttaaca aataaatgaa aaatttaagg
    16441 aaaataaaaa cttaaataag tgaaaaattt aaataaaata aaaactaaaa caaatgaaaa
    16501 atctaaataa atgaaaaagt aaagcaaata aaaaagtaaa gcaaataaaa aagtaaagca
    16561 aatgaaaaat tcaaaataaa aactaaataa aattaaataa aaccaaataa aattaaaata
    16621 aagtctaaat aaaaatttaa taaataaaat ataaagaaat aaaaaatcaa tagattaagt
    16681 gaaatgctaa taaattaaaa aagtgaaata tcaaaataaa acgtcaacaa attaaaaatt
    16741 atatggggga atatcaaaat gttaaatgaa acaagaaaaa aacaattaac tgaagtatgc
    16801 caagacttaa taagaaatgc tagttattca ggtcaagaag aaaacgtagt aaaagctata
    16861 gaagaaaatt ttgctttact aggttttgat agttggtcaa gagatagata tggaaatata
    16921 gtaggatgta taaaaggaaa taaaccaggt aaaaaaatat tatttgacgg acatatagat
    16981 acagttccag ttccagatgc aagtaaatgg agtgttcctc cttttgaagc gagaatagta
    17041 gatgggaaaa tatatggtag aggaacttct gatatgaagg ggcaagtaag tgctatgatg
    17101 gctgctgttt catactttgc agaagatact aacaaagact ttgaaggtga attgtatgta
    17161 gcaggtgttg ttcatgaaga aatatttgaa ggtgtttcag ctagagagat aagtaaggct
    17221 gttcaacctg attatgttgt aataggtgaa tcttcagaat taaacctaaa gataggtcaa
    17281 agaggtagag gagaaatagt agtagaaact tttggtaaac cagctcactc tgctaatcca
    17341 gaaaaaggta taaatgcagt ttataaaatg gctaatgtaa tccaaaagat acaacaatta
    17401 gtacctccaa ctcatccagt tttaggtgat ggaatattag ttttaacaga tataaaatct
    17461 tctccatatc caggagcatc agtagtgcca gattattgca aagctacttt tgatagaaga
    17521 ttattagttg gagaaacaag agaaggcgta ttagctccaa tacaagcttt attagatgaa
    17581 atgatgaaag aagacccaga attaaatgca aaagtttctt atgcagttga aaaagctgat
    17641 tgctatacag gaaatactat agaaagtgaa agattcttcc caggttggtt atatgatgaa
    17701 gaagatgaat ttgttcaagc agcgtataag ggattaaaag aagctggaat agattcagaa
    17761 ataactcaat actcattctg tacgaatgga agtcactacg caggtgaggc tggaataaga
    17821 actataggat ttggtccttc taaggaaaat cttgctcata caatagatga gtatatagaa
    17881 ttagaccaat tattcaaagg tgcagaagga tattatggta tattaaaatc agtttataaa
    17941 aaataattag gagatggttt tgtgagaact ttaataaaac atgggcttat agtagatgga
    18001 aataaaacac cagcatatga aggagatatt ttaatagaaa acgagaaaat attaaaaata
    18061 tcacaagatt taaacgaaga agctgacaaa gttatagatg caaaaggtag agttatatgt
    18121 ccaggtttca tagatactca tagccattca gacctagtta ttttagttaa tccatataat
    18181 gaggtaaaga ttagacaggg aattacaaca gaagtgttgg gccaagatgg tatatctatg
    18241 gcccctctac ctcaagagca tatatcttct tggagaaaaa atttagcagg tttagatggt
    18301 gaaagtgatg aaattgattg gaaatatgaa acaacagaga attatttaaa aatgatggat
    18361 tacaatgggg taggtttaaa tgaaacttat ttagtacctc atggaaacgt tagaatggaa
    18421 gctatgggac ttgaagatag acctgctact aaagaagaaa ttcaaaaaat gtgtgaaatc
    18481 acagagagag aattaaaagc aggagctata ggattgtcta ctggacttat atatatacct
    18541 tgtgcatact cattgactga agaaattata gagatgtgta atgtagtagc aaaatatgac
    18601 ggagtatttg tagttcacca aagaagtgaa gcagatacaa ttttaacttc aatggaagaa
    18661 atcatagaaa ttggaaaaca atcaggagta aaagttcatt tttcacattt taaggtttgt
    18721 ggtaaagcaa actggaagta tataccacaa gttatagagc ttttagaaaa agcagaaaag
    18781 gaaggaataa gagtatcttt tgaccaatat ccatatgcag caggaagtac aatgcttgga
    18841 gtagttttac ctccttgggc acattctgga ggaactgata agttaataga aagattaagt
    18901 gatgaaaacg aaagagcaaa aatgaaaaaa gatatagcaa atggaataga aggctgggat
    18961 aattttatag aatttgctgg aatagaccaa atatttgtaa caagcgtaaa aacagaaaag
    19021 aataaagata caattggtaa gaacttatta gaaattggaa aaatgagagg aaaagaccca
    19081 ttagatgcaa cgtttgattt attaaaagaa gaagaaaatg cagttgggat ggtagatttc
    19141 tatggattag aagaacatat aataggattc atgaaaagag acgagcaaaa tgtgtgtaca
    19201 gatggtcttt tagcaggtaa accacatcca agagcttatg gttctttccc taaaatacta
    19261 ggaagatatg ttagagagtt gaatgtgtta acaatcgaag aagctgttta taaaatgact
    19321 aaaaaggcag ctacaagctt tagtataaaa gatagaggcg aattaaaaga aggatatttt
    19381 gcagatatag ttatatttga caaggataca gttagtggtt gtgatgattt tatcaactct
    19441 atgcagtacc ctacaggtat agattacgta attataaatg gaaattgtgt tgttgaagaa
    19501 ggaaaataca ataaaattaa agcaggaaaa gtacttaaaa attaaatctt agctttaagg
    19561 aggaataaat ttatgatttt agtggggaat gggaggctaa taagccaaga ttctttaaat
    19621 ccctatatgg aagatggatg tgtagtaata aatgataatc taattgaaga tataggtact
    19681 acagaagaaa tgaaagctaa atatccagta catgaattta tagatgcagg caagaaaata
    19741 atcatgccgg gtctaataaa ttcacatatg catatataca gctcatttgc tagaggaatg
    19801 gcagttccag gaaaaccttc tgagaacttt ttagagatac taaacaattt atggtggaga
    19861 cttgacaaac agcttacact ggaagataca aaatacagtg catatgcaac gtatatagaa
    19921 tgtataaaaa atggtgtaac aacagtttat gaccatcatg caagtccaaa tgcaattgaa
    19981 ggcagtttgt ttacaatatc agatgttgca aaggatttgg gaattagaac gtgtctttgt
    20041 tatgaagtgt ctgatagaga tggtggaaat acaatccaac aaggtatcaa tgaaaacgta
    20101 aactttataa aatacgctca aaaagatgat acagacatga taaaaggaat gtttgggctt
    20161 catgctgcat ttacactatc tgatgataca ttaaataaat gtgcttcaga gatggctggt
    20221 cttgatacag gttaccatgt acatgttgct gaaggactag cagacttaca atataatgta
    20281 gataaatatg gaaaaagggt tttagaaaga ttcaatgatt ttggagtact aaaagaaaaa
    20341 acaatagcag ttcattgtat atatataagt gcagaagaaa tcagtctttt aaataaaaca
    20401 aatacatttg tagttcataa tccagaatct aatatgggaa atgcagttgg atgttctcct
    20461 gcaatagagt tattaaaaag gggagttaca gtaggattag gaactgatgg atatactagc
    20521 gatatgtttg aatcattaaa agttgccaat ataattcaca aacatcactt aaaagaccca
    20581 agagttggat ttgaagaatc acaaacaatg ttatttgata acaatagaaa aatagctaaa
    20641 gcttattata aaaatgacct aggagtactt aaaaaaggtg cttatgcaga tgttatagta
    20701 gttgattata taccacatac tccattgaca gaaaatacaa tagggggaca cactattttt
    20761 ggaatgagtg gaagaagcgt agatacaact attataaatg gaaaggtact tatgaaagat
    20821 agaaagttaa taaacataga tgaagaaagt attctagcaa aaagtagaga attaagtcaa
    20881 aatctatgga atagaattta aataattgat attaatatac aagtaagaaa caagaattta
    20941 gatgttaaaa acagagctta aacaaaaata ggaatttaaa tgaagtatat aaataaaaaa
    21001 tatacggaat agaatattaa attaaatata gaagggagac ccaaaatgtc tcgagataaa
    21061 agccctaaaa tagctccagt agatgaaagg ttacctgtat ccaaatcttg ggtattttca
    21121 ctacagcacg ttttagcaat gtgtgcagga gcagtagcag ttcctatgat gataggaact
    21181 gctgcacgac tagatcatgc tagtattgtt tttttaataa atgcttgctt gtttatggca
    21241 ggtataggaa cactcataca aagtgttggt ataggaaata ttgtaggtgc taaaatacca
    21301 gtaatagaag gaactagttt tgccgctgtg gcaggtatga cagcaatagc ctcatcatat
    21361 agtgattcac aagttgcaat acagacagta tttggctctg taatagtagc tggattgttt
    21421 tgtatattac ttgctccagt atttgaaaaa ttaataagat ttttccctag tgtagttact
    21481 ggaacagttg ttatggtaat aggtatttca ctatttccag ttgggataaa atggataact
    21541 gacaacaaat tggaagcggt tgaaccaaac tcattagcat tagctttagc agtattggca
    21601 ataactttac tttcatttaa attcttaaaa ggaatttgga atagcacagc tatattggta
    21661 ggtatagtac ttggtacact actttccatg gcagttggaa tggcagattt tagtgcagta
    21721 aatggagttg gttggcttaa cttaaatgta cctttaaaat ttggaatgcc tagatttgaa
    21781 ccatctgcaa tactatcatt gtgtttgatt atgttggtac ttatgacaga atcagttggt
    21841 aatatgatag ctattcatga aatggtagac aaagaagtaa caggtaaaaa tattaaaaga
    21901 ggtctatttg gagatggaat atctacagca ttatctggaa tattcaattc atttccaata
    21961 actccatttg gtcaaaatac aggaatagta ggtcttacag gtataaaatc aagatttata
    22021 gcagtttacg caggggttat attgctagta cttggattct taccaaaatt tgcagcaata
    22081 ataggagcaa tacctaagcc tgttcttgga ggcgtaggat ttgcaatgtt tggaatggta
    22141 atggttggag gagtaaaaac tttaagcaaa gtgaaatttg atggaactaa aaatggggtt
    22201 atagtagcag ttagtgttgg tttgtctatg ataccactaa caaatcctga tttctatcat
    22261 aatttcccaa cttgggtaca aacaatattc catagtggaa taacaacagg tagtttatca
    22321 gcagtgcttt taaacatatt cttcaatgaa ctaggttcta aaaaagaaaa aaataataaa
    22381 gttgaagaag caaaataaaa ataaaacaag taaaaatcta tattgactta aagtcattaa
    22441 gtattaataa aaataattca gagatattaa taattcaaca aaattatctt atattttaaa
    22501 taatataaaa aagtaaataa ataacgaata caaataaaag aatacaattt aaatattaaa
    22561 aatatttaat ttaagggaga gataataata tggctaattt aaaagttaat ttatatggaa
    22621 tggaattttt aaacccaatt atgacagcgg caggacctgg agcaaaagat ggtgctctct
    22681 gtcaagaggc agcaaaaggt ggagcaggag ctatatgtac aaaaactata tctgtattac
    22741 cagctgatgt accaagacca tgtatggcaa aaactaatag tggtttttta aacacagaat
    22801 tatggtcaga gcttccaaaa gagcaatgga tagaggaaga atacaaaaag gtaaaagaaa
    22861 cagggcttcc agtaatagta agtatgggat attcagcaga gcaaatagct gaggttgctc
    22921 ctttagtaaa gccttatgca gatgcagttg aattatctac tcattatgta ggtacagacg
    22981 ttgcaccaat agtaaatgct ttaaaagctg caaaagctgt tttagatgta ccagtattta
    23041 tgaaaatgag tcctcatact gacattcaaa ctattgcaaa agctgttgaa gaagcaggtg
    23101 cagatggact tgtaatgata aactcatttg gaccatgtat ggcaatagat gtaaatacag
    23161 gataccctat aatgggaagt aaagctggat acggatggtt atcaggtgca ccaataagac
    23221 ctctagcaat aagatgtata tatgaagctt ctcaagttgt aaatatacca ataatcggtg
    23281 ttggtggaat cacttgtgga aaagacgtgg ctgaaatgat gatggctgga gcaagtgctg
    23341 ttcaagtatg tacagaagca atccttaaag ggccttctat ctatggaaaa attgctaaag
    23401 aattaaatga atttttagat gagaatggat atgctgatgt taatgaaata aaaggattaa
    23461 ctcataagaa aatggcagct agagggttta gaactcattc agtagctcca gtagtggatg
    23521 ataacaagtg tatcaaatgt aagcaatgtg taacaagctg tgtatacgaa gctttagaag
    23581 ttacagataa gttgaacata gatgcagaca agtgttttgg atgtggatta tgtgtaacta
    23641 gatgtccaaa aggagcttta agcatctgct acaatcaagt agaagctatg aatgagtaat
    23701 aattatatag aattttggta attaacttag agtaataggt attaggataa taaaatatat
    23761 cagataaaca aacacacatt atgcattaat aatacagtct aaagttaata attttattat
    23821 attgatttaa ctaaatatta gtttagctga atattaattt aattaggtat tggtttaatc
    23881 ttaatataga tttactgcgt ataagtgaac taactagatt actttaagtt aattttttca
    23941 tataaaataa attgatacat tacaagtaag gtggagtcta tatgggaatt gttttaaaag
    24001 gtgggaaagt agtaacaagt gaaaaaactt actgtgcaga tgtaagaata gaagatgaaa
    24061 aaatagtcga tataggtctt gatatatcaa aagaaaatga tgaaatagtt gatgttagtg
    24121 attcttatgt tataccagga ggtatagata cacacacaca ctttgactta gatacaggcg
    24181 ttactaagac agcagataac tttgagacag gaacaaaagc tgctatagta ggaggaacta
    24241 ctactatatt ggattttgct acacaagcta aaggatgttc tttgaaagac ggattagaag
    24301 aatggcatga aaaagctaag gataaatcct attcagatta tggctttcat atggctataa
    24361 cagattggaa tgactcggtt tgtaatgaaa tggaggatat ggtgaaagag ggagtatctt
    24421 catttaaact gtatatggcg tataaaggaa gccttcaagt tgatgatggt gtaatctttg
    24481 aagcattgag aaaagcagaa gagataggag ggattattgg tttccattgt gaaaatgggg
    24541 acataatatg tgaacttgtg gataaagcaa agtcagaaaa taatttatct ccaaaatatc
    24601 atcaattaac tagaccagct ataatggagg cagaagctac atcaagactt atgaagatag
    24661 ctgaagttgc aaactctaaa gtatatgtag ttcacttaag ctgtaaagaa tccttagagg
    24721 aggttttaaa agctaagcaa agaggtgtgg atgtaatagt tgaaacttgt cctcaatacc
    24781 ttctattaaa tgatgaatta tatgatttag atggatttga aagtgctaag tatgtaatgt
    24841 caccaccttt aagaaataaa gaaaataatg aaatattatg gaatgctatt ttagataatc
    24901 aaatagacac tataggaaca gaccattgtt cttttaatta taagggacag aaggaacttg
    24961 ggataaacga tttcagtaag ataccaaatg gttctccagg tgtagaacat aggatgagtc
    25021 ttttatacac atatggagtc tgtaaaaata aaatatctat caacaagatg gtagaagtaa
    25081 catcaactaa tgcagcaaag ttgtttggaa tgtatcctaa taaaggaact atagaaatag
    25141 gcagtgatgc agatatatta gtcttaaatc caaacaaaaa atccaaaata gaagcaaaaa
    25201 aacaattaca aaatgtagat tatacacctt atgaagggta tgaagtggat tgtcaaatag
    25261 aacatgttta tttaagagga aaagaagtag ttagagatgg agaattaatt tgtaatactc
    25321 caagtgggag atatataaat aggcgtctaa caaagggggc tagagaatgg tagaatttat
    25381 attaaatgga gagaaagttg ttgcaaaaga aggcaaaaaa cttttagact tccttagaga
    25441 agatatgaat ataacttcag ttaaaaatgg atgttcagaa ggtgcttgtg gaacatgtat
    25501 ggttataata gatggtaaag ctactaaagc atgtgtattt aaaactgata aattagctgg
    25561 gaaaaacata acaactatag agggattatc agagagagaa agagatgttt ttgcatattc
    25621 ttttgctagt caaggagctg tacaatgtgg attttgtatt ccaggtatgg taattagttc
    25681 aaaagcactt atagacgtta ataataatcc tactgaagat gatataaaga aagctttaaa
    25741 aggtaatata tgtagatgta cagggtatgt aaaaataatt aaagctgtcg atttagcggc
    25801 aaagattttt agagaaaata tagatgtgcc aaaagtaaac tgtaagggtc tagtaggtga
    25861 aaatcttcct agagttgatg cagttggcaa agcacttgga tattctgaat atgtagatga
    25921 tatgagaatt gagggtatga tttatggttc agcagttaga gctaagtatc ctagagcttt
    25981 agttaaaaaa atagatatag aagaagcaaa agcttatcca ggagttgttg cagttctaac
    26041 agctaaagat attccaggaa ctataaaggt agggcactta aagaaggatt gggatacttt
    26101 aataccagaa ggtgaaataa ctagatactt aggagatgga atagttttag tagcagcaga
    26161 aactagagaa gcgttagaag aggccaaaaa gctagttaag atagattatg aggaattaac
    26221 accattaagt actacagcag atgcacttag agaagatgct ccaaagatac atgagacagg
    26281 gaatatactt gttaaggaac atttagtaag aggaaacgct aaagaaaaga tagaaaattc
    26341 aaaatatgtt gtaactaaaa gatatactac acctgctaca gaacacgcat ttttagagcc
    26401 tgaatgtgca gtagctggtc cttatgaaga tgatggagta ataatctatt caacagacca
    26461 aggggtgttt gccactcaac atgaatgtgc agatatgtta gggttaccat atgagaaggt
    26521 aagagttata aacaaaatgg taggaggagg gtttggtgga aaagaagaca tgagtgttca
    26581 acaccatgca gccttacttg cataccatac aaagagattt gtaaaagtac agttaacaag
    26641 aaaagaaagt attattgttc atccaaaacg acatgccaca gaaatggaat ttacgacagc
    26701 atgtgatgaa aatggatact taacgggtat gaaggcaacg ataatatctg acacaggtgc
    26761 atatgcctct cttggaggac cagtgctaca aagactgtgt actcatgcag caggaccata
    26821 caattatcaa gatattgata taacaggaac agcagtatat actaataacc ctcctggagg
    26881 agcatttaga ggatttggag ttacacagag ttgttttgca actgaatgta atctaaattt
    26941 acttgctgaa atggttggaa tatctccttg ggagataaga tataaaaatg caataagacc
    27001 tgggcaagtt ttaccaaatg gtcaatttgc agatgaagga acagcaatgg cagagacact
    27061 agaagctgta aaagaagagt atgaaaagca tccacgtgca ggtatagctt gtgctatgaa
    27121 aaatagtggg gttggagttg gtatacctga tgttggaaga tgtagattgg tgataaaaga
    27181 tggcaaagtt cacataagaa caagtgcttc ttgtataggt caagggcttg gtactgttat
    27241 gactcaagta gtttgtgaaa ctgctaacct acttcctgaa aatatagttc atgaagttcc
    27301 agatactcat ttaacaccag actctggtaa tactacggct tcaagacaga cagtatttac
    27361 gggagaagct actaaggttg catcaaataa gttaaaagaa gctttagact ctggcaaaac
    27421 tttacttgat ttagagggag aagagtttta tggagaatat gaaagtatta cagataaaat
    27481 gggttgtgac aaagaaaatc cagtaagtca tgttgcttat ggatatgcaa cacaagttgt
    27541 aattctggat gaaaatggaa aactagaaaa agtagtagca gcacatgatg ttggaagagc
    27601 tataaatcca atcagtttag aaggtcaaat agaaggtgga gtagttatgg gtcttggata
    27661 tgctctaacc gaagattacc cagtagttaa ctctattcct acagctaagt ttggtacatt
    27721 aggtttattt aaatctacag atataccaga aatacaagct ttaatagttg aaaagaacaa
    27781 agatttattg gcttatgggg caaaaggtgt aggagaaatt tgtacagttc ctactgcacc
    27841 agcagtacaa aatgcctatt atgtatttga taaagaattt agaactgagt taccactaca
    27901 aaatactcca tatagtaaaa aaaagtaaca aaaaaaagaa atattaaata aaaaaataga
    27961 tgaaattgta aataaaaaaa acccttttgc ataaggtata ttttaaatga tagaatatac
    28021 atatacagga gggtttttct tttataaaat aagtttaata aaattgagag gtgagcagaa
    28081 tgaattttaa taatagtcta gaattaactc agtctcagaa gctaataatg actacacaac
    28141 ttaagcaatc attatcaata ttaaatatga gtaagttaga gttagaagaa gagataaaaa
    28201 gagaagcaga gaataaccca ttagttgaag tcgagaaaag tggggagata aattgggaag
    28261 agtatataaa agacatggat aggtcaagac gtttagatag aacagagata agctataatc
    28321 ctgataatga agttaatcta gaaaatttag ttaaatattc gtcaaattta tatgaagatt
    28381 taaaatttca gataagcctg tataaactta cagacaaaga attggaagtc tgtgagtata
    28441 taattgatag tttagatgag gatggatatt taagaactga ggaaaaagta attgttgata
    28501 ccttaaagat agatgaagaa ctttttgaaa aatgtttaat tagtgttcag cagttggaac
    28561 ctagtggagt aggggcaaga agtctttcag aatgtctgat gatacaaatg gctagtttag
    28621 gaatatataa tgaaatttta gaggaaatcg ttacaaaaga cttaaattta attgcaaata
    28681 ataaatacaa agagataagt aaaaagtaca atatgtcatt acaaaaatgt gttgaactaa
    28741 taaacataat taagacatta gaccccaaac caggaagaac ttgttcagta gagaaaagtg
    28801 tatacattca acctgatgta acagtagaaa aaattgatga tgaatttatt gtatatttaa
    28861 atgagaaaga ttcttatcaa ataagaatca atagttatta taaagacata ttaaaaaatt
    28921 cacagtctga tgaaagtgca aaagaattta taaaagaaag actaaattca gcaacaggac
    28981 ttatgaaaaa tatagagagt aggaaaacta cagtacttaa gattgcagaa gagatagtaa
    29041 aagctcaaga tgaatttttg agaaaaggga caaaatatat aaaacctttg aaaatgaaag
    29101 acatagcaga aaaattagaa tttcatgaat ctactataag tagaggtgta aatggtaaat
    29161 atatgctaac accttttggc gtatatgaat ttaggtactt tttcagtagc gccattgaga
    29221 ctgagaataa tgaaatggca tctagtacaa gcataaaaaa aattataaag gaaacaataa
    29281 aagacgaaaa taaaaagaaa cctttaagtg atgaccatat atctaagtta ttaaaagaaa
    29341 aaggaatcaa tgttgcaaga aggacagttg ccaaatatcg tgaagaatta ggaatcccat
    29401 cttcaagtaa gagaaaagaa tactaataag taaaagacaa atatcagtat ggtaagggga
    29461 aaaggctaat taagagatga atgtagattg ggctaaaaat tttataaagt aaaataatat
    29521 aatatgaaaa agtattaaaa tgctataagg tattaaaaaa ctggggaaca ttaaaacatt
    29581 aagatagcaa agaacataaa aaactaaaga agattaaaac attaaagaac attagagctt
    29641 tgaggaatat tagaacttta aagaatatta aaatttataa aatataaaaa tactaaaaag
    29701 aattgaaaaa ctggggaaca ttaaaatatt gtaaaatgct aaaaaactag aaaacataat
    29761 taaataagtg cttataaaga atgattaaat taaataagtg tttgtaaaga ataattagat
    29821 attagaaaaa gaagaatacc tagataaata aactattatt aaatttaata atcacaaaag
    29881 gaaaacacgt agggtataaa taaaaatctt aactagataa aagtaaactt agttgtagat
    29941 aaaaataact agaaataaaa ttatttatag taataaatat tagaatgaaa atataaaaaa
    30001 atatatataa aaagccttat ataaaaagaa aaaatactgt gatttgaaaa tggtggagaa
    30061 atattttata aaataaaaaa ataaaaaagt ttttgaaaaa ctattgaaat ctatacgcaa
    30121 atcctataaa ataaaaatat cttgtgggac ttaaataaac actagggaca aaaaaagtcc
    30181 cgaaaggaaa gcaataagga gtatgttatg aagaacttat tagaaattca aaagaaattg
    30241 attccacagg caattgaact tatggaaaga agatactcta ttttaagaca aatatcttta
    30301 agtgaaccaa taggtaggag aacattatca aatgtattag agattagtga aagaataatc
    30361 aggtcagaaa ctgagttttt aaaagaacaa ggacttatag acgtagctgt atctgggatg
    30421 actatcacta aagaaggttc tgatttgtta gataaactta aggacatcat gagtgacatc
    30481 atgggattgt ctaagttaca agatagagtc agagaaaagc ttggaataaa aagggtaata
    30541 ttagttcctg gaagctgtga tgaaaatgga agtttactaa aagatgtaac tagatatgga
    30601 tgcgaatatt tcttaggcat tttaaaagat ggagatatcg tttcaataac aggaggaagc
    30661 actatgttgg agttttcaaa cgttattaaa actgataaga gatatccaaa ctctacaata
    30721 gttccagcta gaggaagtat gggaactgat gtagagatgc aatctaacaa tatagttgct
    30781 tcaataagta agaaattaca ttctaattgc aaacttttac atataccaga tgagttggat
    30841 gaaagtgcaa tgaaaactct tagccaagtg ccagagatta agacaacttt ggagtatatt
    30901 aaaagaacag acattttagt ttttagtata ggtagagcag atgtcatggc aaagagaaga
    30961 aaacttccag aagacaaggt tgatgagata ttatctaaaa atgcagttgg agaagcattt
    31021 ggatattatt tcaacaaaga tggagaaata gtttacaaac tgaacactgt tggaattgat
    31081 ttagaaacag ttaaacaagt aaaagaaact attgcaatct ttgcagggac tgaaaaagta
    31141 gaagccttat tagctatatc aaatatgcat aaaaacatgg ttcttgtaac agatgaagaa
    31201 agtgcttata agatattgtc attattataa aagtcaagac ctagcacaga gctagaaaac
    31261 atacatttac tttataggag gtagcgaaaa tggttaaagt agctataaat ggatttggaa
    31321 gaataggaag attagcatta agaaaaatga tggagcaaca agataagttc gaagttgttg
    31381 caataaacga cttaacagac gctaaaatgt tagctcattt atttaaatat gatacagctc
    31441 aaggaagatt taacggagaa atagaagtta aagaaggtgc ttttgtagta aacggaaaag
    31501 aaataaaagt aacagcagaa agaaatcctg ctgacctacc atgggctgaa ttaggagtag
    31561 atatagtatt agaatgtaca ggattcttta catctaaaga taaagctgaa gctcatatac
    31621 aagcaggggc taagaaagtt gttatatctg caccagctac tggagattta aaaacaatag
    31681 tattcaacac aaacagtgac atattagatg gttctgaaac agttatatca ggagcttctt
    31741 gtactactaa ctgtttagct ccaatggcta aagtattaaa tgataaatac ggtatagaaa
    31801 aaggattaat gactactata catgcataca ctaatgacca aaatacttta gatggtcctc
    31861 atccaaaagg tgatttaaga agagctagag ctgctgctgg aaacatagtt ccaaacacta
    31921 caggagctgc aaaagcaata ggcttagtaa taccttcatt aaaaggaaaa ttagatgggg
    31981 cagcacaaag agtaccagtt gtaactggtt caataactga attagtttgt acattaggaa
    32041 aaaatgttac agttgaagaa ataaatgcag ctatgaaaga agcttcaaac gaatctttcg
    32101 gatatactga agaaatgtta gtatcttctg acataatcgg aataagctac ggttcattat
    32161 ttgacgctac tcaaacaaaa gttatggaag tagatggaaa acaattagtt aaagttgttt
    32221 cttggtatga taatgaaatg tcttatactt ctcaattaat aagaacttta ggatactttg
    32281 cacaattagc aaaatagtca gttttgagaa aatagaaaag tagattgaca aagtccgagt
    32341 ttgtagttta atactacgga ctcaggactt tttttaagaa aaaatatata aaatacacct
    32401 aaataatgtt acatactata tataaaaata tgataatatg atatatttaa tagggtaaaa
    32461 gtatgtacta tattatactt ggcaataaaa aattacttta ttaaaaggag agatatttat
    32521 gtcaatgctt aacaaaaaaa ctatagaaga tattgatgtg tgcggaaaaa aagtattagt
    32581 aagatgtgac tttaatgtgc cattacaaga tggtgtaatt actgatgaaa atagattgaa
    32641 cggagcttta ccaactattc aatatttaat ttctaaaggg gcaaaagtta tattatgctc
    32701 tcacttagga aagccaaaag gagaagctaa accagaatta tctttagcac cagtagcaaa
    32761 gagattatct gagatgttag gaaaagaagt tgtatttgca gcagacgata atgttgtagg
    32821 agaaaatgca aagaaagcaa ctgaaaaaat ggaaaacgga gatgtagtat tattagaaaa
    32881 tactagatat agaaaagaag aaactaaaaa cgaagagaat ttctctaaag aactagcatc
    32941 acttgctgaa atatttgtaa atgatgcatt tggaactgct catagagcac attgctcaac
    33001 agttggtgca ggcgagtttt tacaagagag agtttgtgga tacttaattc aaaaagaatt
    33061 aaaattctta ggagaagctg ttgcaaaccc tgtaagacca tttacagcta tacttggagg
    33121 agctaaagtt tctgataaat tagctgttat aaacgaatta ttagaaaaag ttgataatct
    33181 aataataggt ggaggaatgg cttatacatt cttaaaagct cagggttatg aagtaggaac
    33241 ttctttactt gaaatagata aagttgaata tgctaaagaa atgatggaaa aagctaagaa
    33301 taagggtgtt aacttattat taccagtaga cgttgttatg gcagaccact ttgcaccaga
    33361 tgctactcca atagtaacag aagatgctaa tgtaaaagaa gattacatgg gactagatat
    33421 gggacctaag actatagcaa actttgtaaa aactataaaa gaatcaaaaa ctgtagtatg
    33481 gaatgggcca atgggagtat ttgaatttga aaactttgca aatggtacat tatcagtagc
    33541 tagagctatg gcagagttga ctgatgcaac tactgtaatt ggcggtggag atagtgcagc
    33601 agcagttaat caattaggat ttggtgacaa gatgactcac gtatcaacag gtggaggagc
    33661 atcactagaa tttttagaag gtaaagaatt accaggtatt gcagcattag ataataaata
    33721 aactaatata tagaggagat tgtaaaatga gaaaacctat aattgcagga aactggaaaa
    33781 tgcataaaac aataaaagaa gctttagaat ttgtaaatga agttaaagat aaagtaaatt
    33841 ctgataaagt agaagctgtt atatgtgcac catttacttt attaaaggat ttaaaagaag
    33901 ctactaaggg tacagatata aagataggtg ctcaaaatat gcactttgaa gaaaaaggag
    33961 catttacagg agaagtttca cctcttatgt taaaagaaat agatatggac tatgtcgtaa
    34021 taggacattc agaaagaaga caatacttca atgaaactga tgaaactgta aacaagaaag
    34081 ttttaaaagc attagaagta ggaatagacc caatattatg tgttggagaa actttagaac
    34141 aaagagaagc tggaaaaact aaagatgttt gtaaagttca agttgaaaaa gctttagaaa
    34201 atgttcttaa agatgattta gcaaaagtag ttgttgcata tgagccaatc tgggctattg
    34261 gaactggtaa aactgcaact gctgaagatg ctaatgacgt tatatcttat ataagagaag
    34321 ttataaaagg attatatgga gaattagcta atgaagttag aatacaatac ggtggaagtg
    34381 ttaaaccttc aaatgtagcc gaaataatgg gtcaaagtga tatagatgga gctttagtag
    34441 gtggagctag tttagctagt aatgattacc tagaccttgt taatttctaa ggagtgaaat
    34501 gatgaaaaaa ccagttgctt taattattat ggacgggttt ggatacaata aggatgttaa
    34561 gggaaatgca attgctgagt ccaaaactcc taatttagat agaataaaaa aagagtatcc
    34621 aaacacattg ataaatgcta gtggtcttga tgttggtcta cctgatggtc aaatgggaaa
    34681 ttcagaagtt ggacacacta acataggtgc aggtagaata gtatatcaag atttaactag
    34741 aataacaaaa tctataaaag atggtgactt ttttactaat aaagttttat gtgaagcaat
    34801 ggataatgca aaagaaaatt ctcttcatgt aatgggactt ttgtctgatg gaggagtaca
    34861 ttcacatata gaccatctta aggctataat caaaatggca aaagataaag gcgtacaaaa
    34921 ggtgtatgtt cacgcattta ctgatggtag agatactgac cctcaaagtg cattagaata
    34981 tgccaaagaa gtacaagcta gtatggatga aattggagta ggtgaatttg ctacagtttc
    35041 tggtagatac tacgctatgg atagagataa gagatgggaa agagtagaac ttgcatacaa
    35101 tgccatggtt agaggtattg gtgaaaaagc aaattctatt gaggaagcta ttcaaaactc
    35161 ttatgatgat ggaaaaaatg atgagtttat aatgccaaca gttattatga aagatgataa
    35221 accagttgga agtataaagg aaaatgattc aattatattc ttcaacttta gaccagatag
    35281 agctagacaa ataactagag ctttagtttg tgaagagttt gatggattta aaagagaaga
    35341 tattaaaaac ttctttgtat gcttaacaga gtacgatata actatagaga atgtgcatat
    35401 agcatttgga cctcaatctt tagccaacac tctaggtgag tatctagcta aaaatggaaa
    35461 aactcaactt agagctgctg aaactgaaaa gtatgcacat gtaactttct tcttcaatgg
    35521 tggagtagaa gagccaaata aaggtgaaga aagactattg ataccttctc caaaggttgc
    35581 aacttatgat ttaaaaccag aaatgtcagc atatgaattg acagataaag ctttagataa
    35641 attaggagaa gataagtttg actttatagt attaaacttt gcaaatcctg atatggtagg
    35701 tcatacagga agtatagaag cggctataaa ggctgttgaa actgtagata cttgtgtagg
    35761 aagattgata gataaaatag tagagttagg tggaagtgca ataataactg ctgaccacgg
    35821 aaatgctgaa tatatgttag acccagaaac aggaaaaact gtaacggctc actctattaa
    35881 tccagtacca tttattgtag ttggtcaaga atatgaaagt gcaaaattat tggatggagg
    35941 aagattatct gatatagcac cgactgtttt agatatgatg aagttagaaa agccagaaga
    36001 aatgactggt cattcactta tttcaaaata gtcgattctg ataagtagag aataatatat
    36061 aaactacaat ataaatttaa aaataatgca gtttaggttt attttgtaaa gtttagactt
    36121 tatgacataa gatttaaatt ttgtaagttc tggatattgt aaggtgtaga tgaagatata
    36181 ttttataaaa tttacaagat agacaattta taaaataaaa aattacaata tagataataa
    36241 tgtaaataat gatataaata ataacaagat agacaataac aatataaata ataataatta
    36301 atatatatta tataagcaaa aattttagaa tcattagttg tacattttat gtttaatgtt
    36361 ttaaatctag ttataatatt tgtgtattta agattggttt tggattttag acaagttttt
    36421 ttatattata taaattaaaa tattataaaa aataaaataa aaataggaac ttttacaaat
    36481 ttacccatca aataaatttg tacctaattt tcaaaagcaa ctttatgtac tataatatag
    36541 aaagtagatt aaaatagtag acggatgttt acagtaaatg aaaatttaac taatagaaaa
    36601 tcgaagggag atatacaaat gtcagttatt gaattagtat atgctagaga agtattagac
    36661 tcaagaggaa atccaactgt agaagttgaa gtagtattag aagatggagc aatgggaaga
    36721 gcaatagttc catcaggagc atctacagga gcttttgaag ctgttgaatt aagagatgga
    36781 gataaaggaa gatacttagg aaaaggtgta gaaacagctg ttgctaacgt aaatgaaata
    36841 atagcacctg aaatagaagg aatggatgca tttgaccaac cagctataga tgcaatcatg
    36901 atagaattag atggaactcc aaacaaaggt aaattaggag ctaatgctat attaggtgtt
    36961 tcaatggcag tagctagagc agctgctgat gaaataggtt taccattatt ccaatactta
    37021 ggtggagtaa atgctaaaca attacctgtt ccaatgatga acatattaaa tggtggagag
    37081 catgctgata ataacgttga tgttcaagaa tttatgatat taccagtagg agcttgttgt
    37141 ttcaaagaag gtttaagaat gggagcagaa gtattccatt cattaaagaa agttttagga
    37201 gaaaaaggat tagcttgtgg tgtaggcgac gaaggtggat ttgctccaaa cttaggttca
    37261 aacagagaag ctttagaatt aatagttgaa gctataacaa aagctggata taagccaggt
    37321 gaagacgtta tgttaggtct tgacgttgct gctacagaaa tgtacaacaa agaaactaaa
    37381 aaatacgttt tagcaggaga aggaaaagag ttaactgctg ctgaaatggt tgctttatat
    37441 gaagattggt caaacaactt cccaataata actatagaag atggtttaga cgaagaagat
    37501 tgggatggat ggaaattatt aactgaaaaa ttaggtaata aattacaatt agttggtgac
    37561 gacttatttg ttactaacac agagagatta gaaaaaggaa ttgaaaatgg agtagctaac
    37621 tctatattag ttaaagttaa tcaaataggt acaataactg aaactttaga tgctatagaa
    37681 atggctaaga gagcaggata tactgctgtt atatctcaca gatcaggaga aactgaagat
    37741 tctactatag cagatttagc tgttgcagta aatgctggtc aaataaaaac tggtgctcca
    37801 tcaagaactg atagagttgc taagtacaac caattattaa gaatagaaga aatggttgga
    37861 gagcaagcta gatattgtgg attaaaatct ttctataact taaaaaaata gttattaaaa
    37921 tggttactaa aaaagattaa tgaagagaga atataagata tttaagctca tcttataaga
    37981 tataaaagag ataagtgtta attgattttt caaggcactt atcctttttt atattttatt
    38041 acttaatttt attcatagat tcagaaaaaa tatgaataaa tgtttctatc tcatttttat
    38101 ctaattgcat aggaggtttt tattaaaaaa taaaagtgaa gacatatttt gatttaatca
    38161 taattgtcat taaagtatta ttgctaaatg tggattattt attttaaatt atgttgaaat
    38221 taaaagttat atcaatattt taaaatgatt gttttatttt ggtggctatt tataatatat
    38281 gcaatgagag tagattattt taagtgttaa tttatgcatt aatttatgta ttaatttaaa
    38341 tagttttctc ttctaagttt tattcatttt tatattgaga atatagtaat atattatagt
    38401 aacaatatat ttttttgtat gttatttata gcataatagt cttgaaatgg ctatattatt
    38461 gtgatacact aaagagtaat gaaatttaag taataaacag ataggagggg caagaatgag
    38521 caacatttta atgggtgtgc aagtagtact agggatttta ctagtaatta gcataatgcc
    38581 tcaagattct aagaatgctt taccttcaga atttggtgga gaaggaagcc aagcttactt
    38641 taaaccaaaa ggaaaacaag catttcttgc tagagttaca aagataacag cagtattatt
    38701 ttttataaat gcattggcat tacttatagt taagaagtag tgatattgtt gaataactgg
    38761 ccattattta attagtgcat ttgctagaca tttaaaaatt aaatttagtt tttaataaaa
    38821 gtacatcaac ataaaaagta cgtaaaataa aagctggact aatgatttta tatgattagt
    38881 ccagttttta ttttatagat tgactatttg ttggcgttta ttatttgtat ttatatattg
    38941 tttgtattta tatatttttt tcaaactaaa aaatatatag caacactact ttcaccatgt
    39001 ttaatgagat aatatacaca aagttatatt atatagtaaa aatagtataa taatatgttt
    39061 ttacgcaatt aaatcgattt tatatatgtt tctcataata ccaatttgtg ttatcaaata
    39121 tcattaaata agattattct gctttaaaat aaatattatt aaaaatactg taagtataga
    39181 aaatccagaa gtaaatacta caatttgtcc agctaattca ctgtcagaat ccatctgttg
    39241 tgccattgta aatgaagaaa ctgcagttgg agcagataac attatcataa gtgaagctaa
    39301 ttctacattt ctaaatccaa gaaaaatgct aattggtaaa aatatagcag gtacaagtat
    39361 aagttttcca catacgccca atataatggg ttttatatgc ccctttatag cagaaaaact
    39421 aaaagagccg cctagaatta taagtgataa aggagtagct attttagaaa tatcaccaat
    39481 agttttgtgt aaaaatgtag gtatattaat atttaaaatt agcattgtca atcctataaa
    39541 agaggcgatt atgagtggat ttgttaagac gcttttaatc atatgtttta tatttacttt
    39601 gccacctcta aaaatttcaa gaattacaac agataataca ttaaacatag gtacaataac
    39661 tgcaattaat aaagatgtga ctccaatact cttatcgcca aataatgaaa ttgtcacagg
    39721 aataccaaat ataacgaaat tgctacgaaa tacaccttgt attaatgcac cccttgtctt
    39781 attatctttt tcaattttag gaataataaa gactaagatt aagaagattg aaaaaataat
    39841 tataacagct acagccataa gcctaaaatt aagagaacca tctaaatctg tattgtatat
    39901 attaaaaaat aataataatg gcaagaaaga cttgaagcag acattattca ttttatttaa
    39961 agtatgagta tcaaatagac ctaatttttt tagaacatat ccaagtgaca ttgtgagaaa
    40021 caaaggaaga acaacattta ttgataggat taaattttcc aaggtttact ctccgtgatt
    40081 ctatgatttg ataattcaat taaatttcca ttagtttttt tcaaataaat agatttcata
    40141 ttaccaaaga cacctgtaca ttctacaatt cctcgcttaa tttgaatctc attttgttct
    40201 aagtattttt ttggctcatc aatatttgtg tttattttaa aacataagtt atctctactt
    40261 gcttttgaat ttttaacata aggtaaaaat ttataacctt ttatgttaag atttatctta
    40321 acatttctag cattaagttt ataatttttg ctcatttctt tagatgaaaa accaaatttt
    40381 ttataaaaat tgatataatt ttctatatta ttattagtaa tgaccacatg ttctattttt
    40441 tttatcatgg caa
//
LOCUS       DAEMIK010000017        40283 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEMIK010000017 DAEMIK010000000
VERSION     DAEMIK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.017"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            129..866
                     /gene="cas6"
                     /locus_tag="KPK87_000759"
     CDS             129..866
                     /gene="cas6"
                     /locus_tag="KPK87_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG2536934.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILSAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLGSIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            871..2346
                     /locus_tag="KPK87_000760"
     CDS             871..2346
                     /locus_tag="KPK87_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536935.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLMYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLKNMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2366..3379
                     /locus_tag="KPK87_000761"
     CDS             2366..3379
                     /locus_tag="KPK87_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG2536936.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3395..4192
                     /gene="cas5"
                     /locus_tag="KPK87_000762"
     CDS             3395..4192
                     /gene="cas5"
                     /locus_tag="KPK87_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG2536937.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4219..6627
                     /locus_tag="KPK87_000763"
     CDS             4219..6627
                     /locus_tag="KPK87_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG2536938.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKNILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQKEIYDYIE
                     KGILEKEDFNLEDIEDYIKEILYVDFKFNEDVLDYDYIDKLSESMVIDSDYNNGQIED
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGENDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKSILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7068..8015
                     /gene="prmA"
                     /locus_tag="KPK87_000764"
     CDS             7068..8015
                     /gene="prmA"
                     /locus_tag="KPK87_000764"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG2536939.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8034..8792
                     /locus_tag="KPK87_000765"
     CDS             8034..8792
                     /locus_tag="KPK87_000765"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG2536940.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8794..10092
                     /gene="mtaB"
                     /locus_tag="KPK87_000766"
     CDS             8794..10092
                     /gene="mtaB"
                     /locus_tag="KPK87_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG2536941.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10135..10485
                     /locus_tag="KPK87_000767"
     CDS             10135..10485
                     /locus_tag="KPK87_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG2536942.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10648..10827
                     /locus_tag="KPK87_000768"
     CDS             10648..10827
                     /locus_tag="KPK87_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG2536943.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10857..11300
                     /locus_tag="KPK87_000769"
     CDS             10857..11300
                     /locus_tag="KPK87_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG2536944.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11411..11908
                     /locus_tag="KPK87_000770"
     CDS             11411..11908
                     /locus_tag="KPK87_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG2536945.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12101..12664
                     /gene="raiA"
                     /locus_tag="KPK87_000771"
     CDS             12101..12664
                     /gene="raiA"
                     /locus_tag="KPK87_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG2536946.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12877..13113
                     /locus_tag="KPK87_000772"
     CDS             12877..13113
                     /locus_tag="KPK87_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG2536947.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13126..14469
                     /gene="yqfD"
                     /locus_tag="KPK87_000773"
     CDS             13126..14469
                     /gene="yqfD"
                     /locus_tag="KPK87_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG2536948.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14408..15523
                     /locus_tag="KPK87_000774"
     CDS             14408..15523
                     /locus_tag="KPK87_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG2536949.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15580..16041
                     /gene="ybeY"
                     /locus_tag="KPK87_000775"
     CDS             15580..16041
                     /gene="ybeY"
                     /locus_tag="KPK87_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG2536950.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16047..16766
                     /locus_tag="KPK87_000776"
     CDS             16047..16766
                     /locus_tag="KPK87_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG2536951.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16885..17286
                     /locus_tag="KPK87_000777"
     CDS             16885..17286
                     /locus_tag="KPK87_000777"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG2536952.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17350..18243
                     /gene="era"
                     /locus_tag="KPK87_000778"
     CDS             17350..18243
                     /gene="era"
                     /locus_tag="KPK87_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG2536953.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18255..19634
                     /gene="mgtE"
                     /locus_tag="KPK87_000779"
     CDS             18255..19634
                     /gene="mgtE"
                     /locus_tag="KPK87_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG2536954.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19707..19862
                     /locus_tag="KPK87_000780"
     CDS             19707..19862
                     /locus_tag="KPK87_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG2536955.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19828..20601
                     /gene="recO"
                     /locus_tag="KPK87_000781"
     CDS             19828..20601
                     /gene="recO"
                     /locus_tag="KPK87_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG2536956.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20594..21193
                     /locus_tag="KPK87_000782"
     CDS             20594..21193
                     /locus_tag="KPK87_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG2536957.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21353..22231
                     /gene="glyQ"
                     /locus_tag="KPK87_000783"
     CDS             21353..22231
                     /gene="glyQ"
                     /locus_tag="KPK87_000783"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG2536958.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22231..24297
                     /locus_tag="KPK87_000784"
     CDS             22231..24297
                     /locus_tag="KPK87_000784"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG2536959.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKIKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24463..26010)
                     /locus_tag="KPK87_000785"
     CDS             complement(24463..26010)
                     /locus_tag="KPK87_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG2536960.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKILHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DTFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26290..27057
                     /locus_tag="KPK87_000786"
     CDS             26290..27057
                     /locus_tag="KPK87_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG2536961.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTIHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27340..27537
                     /locus_tag="KPK87_000787"
     CDS             27340..27537
                     /locus_tag="KPK87_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2536962.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27566..28642
                     /gene="vorB"
                     /locus_tag="KPK87_000788"
     CDS             27566..28642
                     /gene="vorB"
                     /locus_tag="KPK87_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2536963.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28645..29364
                     /locus_tag="KPK87_000789"
     CDS             28645..29364
                     /locus_tag="KPK87_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2536964.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29377..29916
                     /locus_tag="KPK87_000790"
     CDS             29377..29916
                     /locus_tag="KPK87_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2536965.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            30073..31131
                     /gene="buk"
                     /locus_tag="KPK87_000791"
     CDS             30073..31131
                     /gene="buk"
                     /locus_tag="KPK87_000791"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2536966.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31149..32042
                     /locus_tag="KPK87_000792"
     CDS             31149..32042
                     /locus_tag="KPK87_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG2536967.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            32072..33280
                     /locus_tag="KPK87_000793"
     CDS             32072..33280
                     /locus_tag="KPK87_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2536968.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTSE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33304..34563
                     /locus_tag="KPK87_000794"
     CDS             33304..34563
                     /locus_tag="KPK87_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2536969.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            35208..36671
                     /locus_tag="KPK87_000795"
     CDS             35208..36671
                     /locus_tag="KPK87_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG2536970.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            36727..37863
                     /locus_tag="KPK87_000796"
     CDS             36727..37863
                     /locus_tag="KPK87_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2536971.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            38099..39214
                     /locus_tag="KPK87_000797"
     CDS             38099..39214
                     /locus_tag="KPK87_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG2536972.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            39366..40235
                     /locus_tag="KPK87_000798"
     CDS             39366..40235
                     /locus_tag="KPK87_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2536973.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT        16166 a         4084 c         7455 g        12578 t
ORIGIN      
        1 gtagaatata tttaaaaaat taaaaaacaa aattaaattt attatcaaaa aatcatttta
       61 tgatatgttt tatttttata ttacaaatag agaataatga attatactag aaagtaggag
      121 gtgaaaatat gagcagaatt tcaattttta tggaatcatg taatggcaaa tttccaaatg
      181 aaaattcaat gttgtcagtt tcctttataa aaaatatttt aagtgcagaa aacaaagatt
      241 ttgctaaaaa catatttaac tatggggaag aaagaaaaaa caataagcaa ataaaggata
      301 taaatacagc catttacata ccaaatctta taagaagcaa aaatgagtta cttgttgatg
      361 gagatataat attcaatata agtttttata attattctat gttcagtaaa ttatacaatg
      421 gaattttaaa agtaaaggag ctagaatata aaggttatag atttaaaata aaaaatataa
      481 aaatacataa agaacacgag ataaaagaaa atggtgttat atttaaaact atgtctccta
      541 taatagtaaa gaataaagaa ggtaaatatt tagatgtaga agattcaaat tatatagaat
      601 gtttaaacta tatagctaat ctaactctgg ggagtataag aggtagtggg cttagaaagc
      661 cattggaatt tatacctatt aacttcaaaa aaagagtgtt aaaagaaaag attcgtggat
      721 ttaaagtgag agaatattac tatataaatg cttatgcagg aacatttttt ttaaaaggag
      781 atatggaaga tttaaatgca ctgtataaaa tgggaattgg atatagaagg acagaaaatg
      841 caggaatggt tgatatatta aagtagcgag gtgagagaaa taaaagctaa attttataga
      901 tcaggtgata ttgtaagaga tattggcatt gtttactttt atgaattact aatggattta
      961 aaaaacgaac tagaaaaaaa tgattataag ctaaaattta cttgtgagtt aaatagaaat
     1021 tatttatctt tagaatctgc agaatatata gaccctaagt atatatctga ttatatatta
     1081 gaaaatcaac tttttaaggt atttaaaaat gggaaaaagg aaaccttaga aaaaatattt
     1141 agtaatatag ataatttaat gtatgtaaat tttattgaag aattggataa atcttctgca
     1201 actgctaaac aaaaggaaga tattaaaaaa gattttaaaa acaggaggtt tccatatgtt
     1261 agaaatagtg gtaagtatgg attaaacacg agtttaaaaa atatggaaac aaatgtaaaa
     1321 agaatagtag atactgtaat taaatcaaat atagagatag atgaaataga taaacttgat
     1381 ttgacacaat atgaagaaaa agatagtgta tgtaatgttt gtaatataaa taaaacaaca
     1441 aaactagata ttgatttaag agagcgaaag gactctaagt ataacttttt atttagaggt
     1501 gcagaaaaat caggctttaa aagaagtgga caagttgaaa gtaatatttg ttttgagtgt
     1561 gaatttttca acttaatgtg tcttttatat ataaatttaa aaagaccaat ggtttttgca
     1621 tatacgaatg atttaaggga attagcattt ttaaatcata agataatgct taaaagaaag
     1681 atgtattcag ataaatcatt ttataaaaaa cttttgcatg aaaaaataag ttcaattaga
     1741 ctatataggt ttgatattga tacgaataaa ggtataattc taaagtttga ttctattata
     1801 gagtataaag aattattaaa aaaaattgaa cttatagata ttatagataa ttataatttt
     1861 tcaagagagc caggtaatac tagaaatttg ggtaaaagaa tgattgataa tgggaattta
     1921 tcaaatttaa aagaattatt acttgataat ataagtgttt taaagcaatc aactggaact
     1981 tctcgagaaa tggattttgt atctagctca tataatatcg gtttatatat aaaattgtgt
     2041 tggattgtag atggagggga ggagtataaa atgaaaaact atatggaaag taatttaata
     2101 tattcaagag taggtaaaga cttatttaat aaaatgacag atgaatccag aagaaaaaat
     2161 ttttctatga aagtgattca actacttaag tctaatgata gaactcagct atttcaaact
     2221 ataatgcatg tgttagtttc taatggaatc ttaataggag aagggtttgt agaaggtcta
     2281 atgcagagtt ctgaattaga attaaatacg aatgtaggtc tatttattca agaaatcatg
     2341 aaataaaaaa gggggaaaat ttaaaatgaa tgaaaaaaga gtaaaaggag taacattaac
     2401 tatactgaca gaaagcccag ttccactttc atatgaccaa gggtatggaa attatacacc
     2461 aataaaaaaa gaacaatata gagaaaaaat acatgcaaaa actagtatag ccacaataac
     2521 atatgattta agaagaatgc tccaccaaga atatggttgg aatttaagta atattgtttt
     2581 tggaaaaaaa ggaaatatat atccatctat taagaaaaat gtaggatgtg tagatgaaaa
     2641 tggattagag actgatgtat ttggttattt aataccactt aaagataaag gttctataag
     2701 taaagcttct ccacttagaa taataccttt taggtcatta aatccatata aaggttcaac
     2761 acagctaata acaaatagag gctttttaag tagtgaattt ggtagaaaat attatgatga
     2821 gaaggaagaa aatgaagtac ctagagatga aaactttcca acatcacaag cattagcaat
     2881 agaagagact ttatcagatt actacgttta tacaataaca ttggagcttg atagaatagg
     2941 agtagtagaa gtagaagatg gaaagttact tttaccagaa gaaagaaaat ttatgagtaa
     3001 ggagttaaga gaaaaagcag taaaagatat attagatgca attactgtgt ttacaagaaa
     3061 cataaaacat tcatcagtac tattaaaacc acttgcagta ataggtggag cttttgataa
     3121 agttgtacca tttttttggg atgatgtaga ttataatgct gatagtggtg agataaattt
     3181 agagggagtc atagaaacta tagaaagtta tagccttaaa gaaagtaaca caatacttgc
     3241 tatcaatgat agattaaaaa tttctaataa aaaggaatta aataagttta atttaagtaa
     3301 ataccctgta aaagagatta aaaatctagc agatagattg gaaattggag aagataatat
     3361 gtggtattta aaagagtagg aggttttttg aattatgaaa gtattacaat gtaaacttaa
     3421 gcaaccaact gctcactata gagacccaaa agtatttcaa aatgaatata taagtacttt
     3481 aaatttacca tctaagacca caataatggg aatgataacg tatttatgtg atagaagatt
     3541 aaactctgat atagatatag gaataattgg aacacatcat catagagaac tagaattttc
     3601 aagaggagaa aacattgatt tttggaatga atatataaaa atgggaaaag gtaaagataa
     3661 agaaaagttt ttgcttaaag gaaactacta tgattactat aaagagcata aagctcaaaa
     3721 tagcatatta aactatgaag ttcttaaaga agtagaactg actatattta tttcttgcaa
     3781 ggatgatgaa gaacttgaat ttattagaaa aaagcttgaa agtccatgta aatatgcaaa
     3841 tttaggaaga aaagaagatt ttgtaatacc aagtgaaaaa gggtgttttg tgaaagaagt
     3901 taagttaaaa gaggttatac ctatgaatac tcgtgatgct ataaaagaaa atataaaatt
     3961 aaagaataca tatgtaagag ttgacttaag agataaagag aatgtcgaga ctataataaa
     4021 tcaaggtgta ttgattgcat tgcctcataa atataaagat ttagaagcaa atagagatga
     4081 tagagtttat gagttttgcc attatatata tgttgataat gatggtatat atcctaaaaa
     4141 tattaaggta aatgtatgta ttgatacaaa agaagttttt acatggcttt aaattatttt
     4201 gaaagatagg tgtttatcat gaaaaatatc atgcaaaaag aaatattagc taagtcatac
     4261 aatttaagaa ctaatcaaca agagcaaaca ttagttgaac atattaagga tttatttgaa
     4321 gtattagaat ctatattaga actaaatcta tattcagata aagatgtaga gatactaaaa
     4381 atatgttgtg ctttgcacga tttaggaaaa attaattcaa ttatgcaaca aaaaatggag
     4441 attaataata aaatatcgta tagttgttca gaagaagaga ggaaaaagtt agagtctgac
     4501 aaaaaatctt taaaaaagat agcaagacac aatatatttt ctggagcatt tttaaaaaat
     4561 atactagaaa aaatgaactt gtcagaagaa gataagttat atatatataa aagtataatg
     4621 ttacatcatg gaaactatga agattatatg aggttaagta ctagtaaagt tcaaaaagaa
     4681 atttatgatt atattgaaaa aggtatttta gaaaaagaag attttaattt ggaagatata
     4741 gaagattata taaaggagat tttatatgta gattttaaat ttaatgaaga tgtattggat
     4801 tatgattaca tagataaatt aagtgaaagt atggttatag acagtgatta taacaatggt
     4861 caaatagaag attcagtttt aaattataga aagtttaagt atattttata taaagggatg
     4921 ctgaacttaa tagaccatag tgcatcaagt agacagaaag gtataaaatt ttataacgat
     4981 tttactgatg aagaaattga taatatgatt ttaaatgaga tatataagag tcagggtaat
     5041 ttagaaaaaa ataatataga gttcaatact atacaaaaaa ggttgagaga actttctgga
     5101 agaaatgttc tgactgttgc atttacaggt tcaggaaaaa cagctgcaga ttatagaaag
     5161 acttttaaaa gaaaattctt tttagtgcca aataaaatat cagcagaaag tttttataga
     5221 aaaaatattt ttcaaaataa aaatgattta gatacaagaa gtagtaatga ttacattgga
     5281 ttacttcatg gagatataaa cttatattca gagaatgaag ataatggaga aaatgatttt
     5341 gtattgacat taagggatat agatttgagt ataaactttt gcaaaccttg tgtaattgca
     5401 acagttgacc agttattact tagtatgttt aaatttccgg gatatgaaaa aatattcgca
     5461 gccgtaaaaa atgcatctat aactgttgat gaagtacatt tattggaacc aaagatgttt
     5521 ttgattttga tatattttat gcagtttgca tgcaagtact tagatgttga ttttcactta
     5581 atgacagcta cacttccaaa atcatataaa gagcaaatga taaacaaggg aattattttt
     5641 caagaagaaa gtaatgaaaa tgttactgat aaaggagaaa tagtttttat tgagagcaac
     5701 aaggaagaag agatttgtga aggtaaagat gtaaaggttt cttttataaa ggaaaaagaa
     5761 atcaagtcaa tagttgaaga tgctttagaa aataaacaaa aaattcttat aataaagaat
     5821 actattgatt cagtcaatag aacatatgag tttttaaaag aaaatttatc tgataagtat
     5881 ggtggtgtag atatagatgt tttacatagt agatttaagt tcaaagataa aaaagagaaa
     5941 tatagtaaaa tattaaatgg agagggtgat atttggatat caactcaatc agtagaaata
     6001 tccttggatt tggatttcaa tataataata agtgatttag caactatgga tagcttaata
     6061 caaagaatgg gtagatgcaa tagaaataat aaatatgaat atggaaactt ttatattcta
     6121 cctagtgaag ataaaattta tgataacaaa cttaagaaca caactaaaag catcttaaag
     6181 gacatattaa aaactaactc tatttttaca atggggatta gaaaaactat tttggatgat
     6241 tactatgata atagtgtagt taaaaaatat tttgaagaga attttataag ttgtgataaa
     6301 gaaattaaga atatttatgg tataaataaa gaaatttttg atggattgga tttaattttt
     6361 aattttgaac cttacaaaaa tattgttgat agtaagaagg aagcagctaa aatttttaga
     6421 gatgttgatg ttagttacaa aattattctg gaagaagatt tttataagga agatagggag
     6481 ttacaacaag attcgattca agtatcaggg tttatattta atagattata ttattatgga
     6541 ttaataagca aagtagaagg atacatggta ctaaaatcta gtagtaagtt tgagtataat
     6601 agtactgtag gcttaatttt gaagtgagca tatgtttaaa tataatatac ctttagacat
     6661 tgaaaataca aaaagtacta ctgtaagtct actgtaaaaa ctatttttta aaaggaaaaa
     6721 taagttgtga gatagagtgt ttacaagtgt atatagataa taaatttgag gtttaagatt
     6781 aactaagtgg gtttcaggta catcgcgaaa ataattataa gttggagatt ttgtatgttt
     6841 aagattaact aagtggattt caaatattga tatagtagat ataatagaag ttttattatt
     6901 aggtttaaaa ttaactaatc ggatttttaa atattctgac tattatataa ggatattatt
     6961 agattgaatt taaaaccaat aatataaaac aacaaaatat tatatttatg atataataaa
     7021 agttatggaa aaacaggaat attactatag agaagagagg taaacttatg aataattgga
     7081 tagaagtaac tataaaaaca acaacagaag cagttgaacc tataacgaat atactatatg
     7141 aacaaggagc tggaggagct gtaatagaag acccaaaaga ctttttattc caaaagaaaa
     7201 atgaactaga ttgggattat gttgaagaag aagtatttaa aaagaatgaa gaagatgatg
     7261 ttcttataaa aacttatgtt tctgaagaaa aaaatgtgat ggaatttgtc gaaataataa
     7321 agcaaaaagt tttaggatta aaagattttg gtatagatat aggtgaagga agtgtatcat
     7381 tggaccaagt aaatgaagct gattgggcaa atgcatggaa agcatactac aaaccaacaa
     7441 aagtgggaca aagggtagta gtaaaaccta catgggaaga ttatgctatg caagaggggg
     7501 atttaatcat agagcttgac cctggaatgg catttggtac agggacacat gagactacta
     7561 gtatgtgcat tagagaatta gaaaagtatg tgaataaaga ttctaaagta tttgatattg
     7621 gatgtggtag tggaatcctt gcaatagcag cagccaaatt aggagcaaaa gaagttgtag
     7681 ctgtagattt agatgaagtg gctgtaaaag ttgcaaaaga aaatgttctt gaaaataaag
     7741 ttgaaaaaag tgtatctgta atgcatggta atttaaccga tgtaataaaa gataaagcgg
     7801 atgtaatagt tgcaaatata atagctgata taataaagat acttgcaaaa gatgttcaaa
     7861 actttatgaa agaagatgct atattcatat cttcaggtat aattttggat aaagtagaag
     7921 aagttaaaga aagcttaatt gaaaatggat ttgaaatagt tgaagttcaa aaattaggtg
     7981 aatggtcagc aatagtttct aagcttaaaa aataagggat atggtgacgt ataatggata
     8041 gattttttgt tgaaaaaaat aatataaatc tacaagataa aacttgcact atagaaggtg
     8101 aagatgtaaa acatatttca aaagttttaa gatgcaaatt aggtgagaaa cttgaaatat
     8161 gtgacaagaa taataatgag tatatttgtg agataatgaa tatagataag agtatagtta
     8221 atttagagat tttagaaaaa gttgatataa atagagaatc agaacttaaa gtaaggctat
     8281 accagggact tccaaaagct ccaaaaatgg aaatgatact tcaaaaatta actgaagtgg
     8341 gtgtagaaga aataatatta gttcaaacta agagaagtgt tgttaaagtt gatgacaaaa
     8401 aagaagataa aaaatttgaa cgttgggaaa gaataattta tgaagcagca aagcaaagca
     8461 aaagaggtaa gataccaaaa ttaagaggtg ttttaagttt taaagaagct ttagaagata
     8521 tgaaaaaaaa taatgtaaat atttgccctt atgaaaatga aagaacagtt tcaataaaac
     8581 atgcactaaa aaaatgtgat tctaatatag atagtgttgg aatttttata ggtccagaag
     8641 gtggattttc tgaagaagaa atagaacaaa tacaaaaaaa taattgtaat gttgtatcat
     8701 tggggcctag aatattaaga actgagacag cttctgttgt agcatcaaca atagctttgt
     8761 atgaactaag tgatttagga ggagaaaaat aaattgaaaa aagtagcttt ttatacactt
     8821 gggtgtaaag ttaatcaata tgaaacagaa gctatgctag aactatttga gaaagatggt
     8881 tatgaacaag tcaatagtga agagtatgct gatgtttatg ttataaatac atgtacagtt
     8941 actcatatga gtgatagaaa atctcgtcaa tatataagac gagttaaaaa gaagaatcca
     9001 gatgcaataa tagccgttgt aggatgttat tctcaggtat ctccggaaga aattttggat
     9061 atagaagaag taaatttagt catgggtaca aatgatagaa gaaagattgt tgaagaaatt
     9121 aaaaagataa attcaagtaa gaaagttagt acagttgacg acataatgaa agttaaagct
     9181 tttgaagaaa tagaaatcag ccaaactaat ggaaagacta gagcatttat gaaaatacaa
     9241 gatggatgtg acagattttg tacttattgt atcattcctt atgcccgagg acgcgttaga
     9301 agtagagata ttgatagtat agtcgatgaa gtgaaaaaat tggctaataa tggctataaa
     9361 gaggttgtat taacaggaat acatgttgcc tcttatggaa aagatttaaa ggatagggat
     9421 attaaacttc ttgatgtaat aaaacaaata aaccagatag aaaaaataga aagaattagg
     9481 cttagttctg ttgaaccaat tttatttaca gatgaatttg taaatgaagt tttaaagatg
     9541 gataaagttt gtcctcatta ccatttatca cttcaaagtg gatgtgatga aactctaaaa
     9601 agaatgaata gacgatacac aactttagag tacaaaacta ttgtagatag attgagaagt
     9661 aaaatgccag atgtggcaat aactacagat gtaatagtag gttttcctgg agagacaaat
     9721 gaagagttta aaaaaacgta cgagttttta aaagaaatag agctttcaca aatgcatatt
     9781 tttaaatatt caccaagaaa aggaactcca gctgcaacta tggaaaatca agttgaccct
     9841 caaatgaaac actttagaag tgaacaactt ttaaatttaa gtaaagtaaa ttttaataaa
     9901 tttgcaacta aatttatcgg ccgtgagttg gatgttttgt ttgaacaaaa tatagaaggc
     9961 aataaatatg agggattaac ttcaaattat ataagagttg ttgttgaaag tgacaaaaat
    10021 atacaaggtc agatattaaa agtgaaaata aatgatgtaa aagatgaata tgtagaagga
    10081 attttgctat aacatgtaga aattataata tttataagat aggaggtgga aagaatggat
    10141 tgtatttttt gtaaaatagc gaacggagaa ataccttcaa ctaaagttta tgaagatgat
    10201 agagtcttag cttttaatga cttgaaccca gttgctccat atcatatatt agttgtacct
    10261 aaaaaacatt atgatagttt aattgatatt cctgataaag agatggacat tgtgtcacat
    10321 attcatgttg ttattaataa aatagctaaa gagaaaggtt ttgaccaaac tggattcaga
    10381 gtcataaata attgtggaag tgatggaggg caagaagtta aacatttgca ctatcatatt
    10441 ttagctggta aaaagcttcc taattacgaa gcgggacaaa actaaactaa attattgaaa
    10501 aattgagttc cttcttgata aaaatacttg ataaaagtaa aaatttagtt tataataaac
    10561 aaagtgttag gttgtaatgc atattatgca aaaagaacat agttacagtc ccagcataat
    10621 tgcgctattg gagggaggga aaaagaaatg tcagaagtaa gagttagaga gaatgaaaca
    10681 ttagacagcg ctttaagaag attcaagcgt caatgtgcaa tgtctggcat catgtcagaa
    10741 gtgagaaaaa gagagcacta tgataagcca agcgttaagc gtaagaaaaa agctgaagca
    10801 gcaagaagaa aaaacgctaa gaaatagtag taaataagaa taaagaggtg aaggggatgt
    10861 cccttaagca aaagttacaa gaggatttga aatcttcgat gaaaaacaaa gatacagtga
    10921 gaaaatctgt agtaacttta ataagagctt ctataaagca gtatgaggtt gataataggg
    10981 tcgagcttga tgaagatgga attatcgatg taatagcaaa acaattaaag caacgtagag
    11041 atgcattagt tgaatttgaa aaagctggaa gagaagactt aataaaagaa acagaaggtg
    11101 aaattgaagt tttaaaagag tacctacctc aacaattaag cgaagaagag ttagaagaaa
    11161 ttgtaaaatc tactatatct gaagtagggg caacttctat gaaggatatg ggaaaaataa
    11221 tgtcagttat ccaaccaaaa gttaaaggaa gagctgacgg aaaacttata aataagttgg
    11281 taaaacaaaa cttacaatag attgatatag cctagagaga aatctctagg ctttttatag
    11341 tttttgggaa taacgagtca tatttgagca tatctataat caaagaaaat tttgaaagaa
    11401 ggggaaaaat ttgattatat cagtaaaaga gattaggaat tttttgataa acatatttat
    11461 accactaata ataggttact taagtagcat cttaacaatg attatcagtg gaacagatat
    11521 ttcaacctat tatttacaac tcacaaagcc aagctttgca ccaccagctt ttatctttcc
    11581 tatagtttgg actatattgt acatattgat gggtatatct tcatacctaa tattaagaaa
    11641 aggttacaac ttacctaaag taagagatgc aattttttat tatggattac aacttgtatt
    11701 aaactttatt tggagtatac tattttttgg atttggactt agatttacag ctttgataga
    11761 tataataata atgattttgg tttctataat aatgatttcg aagttttcta aaatagataa
    11821 gagatctgga tacataaatt tgatttattt aatgtggctt gtttatgcag gatttttgaa
    11881 ctattttata tggtttatta ataaatagaa tgtaaagatt ttgtaaatta ccctgtactt
    11941 ttaataatat cataacaaac gaaaaataaa tagactatta aaaataatca ttttcatata
    12001 caataatatt aatttaaata caataaattt ataaaaattg tatttaaaga ttaacatatt
    12061 aaagtattag aattggaaag gcttgaaggg agaggttttt atgcaaataa tagtatctgg
    12121 gagacaaatg aaattaactg acggaataaa aggatacgta gatggaaaat taagtaggtt
    12181 agaaaaatac cttgatcctg aatcagaagt gaaagtaaca gtaagtgcga aaaaggatag
    12241 acaaaaagtg gaggttacaa taattcctat taacggtcaa ataataagag cagaagatgt
    12301 agaagatgac ttatatgcag caattgatat tgtttgtgac aaattaagta gacaagttgt
    12361 caaatataaa actaaagtta aagacaaagt tcaaaataat aagagcataa gatttgaaaa
    12421 tcttgacttt atagataata gcagtgaatt tgatgattat gattatgatg atgaagaaga
    12481 tgaaaatata gtaatagaaa gaagaaagaa atttaatgta aaacctatga gttctgagga
    12541 ggcgatactt caaatggaac tagtaggaca caatttctat atgtttagaa accaagataa
    12601 ttttgaaata aacatagtgt ataagcgtaa agctggtgga tatggtttaa tagaacaaga
    12661 ttaataaaaa ttgtatacgt ttagatttta tataagaaaa aataaaaagg taatcatgtt
    12721 gattttttat caatatgatt acctttttct tgtttaaata atatattaga tattaggtat
    12781 ttattatata aaaattttaa aactaatctg ttacaacatc aataaagtgc ataaatacct
    12841 atttcaagca taaaattaat taagggggta atgcttatgt tagaaatatc cactgattta
    12901 caagtaaatc aaccagttat tactgttaca agtaatacgt ttattagtat agaaaattat
    12961 ctatcaatat tagaatatga agttgattta ataagaataa agactaaagt taaaacaatt
    13021 aaaatatctg gggataaact ttcacttaag tatattactg atagtgagat aggtataaaa
    13081 gggattatct acaatgttga gtatgttgat taggaggaga tacacttgat aagttttata
    13141 aggggatact atgttatagt tgtggaagga gtaggactcg aacaattttt aaatcatctt
    13201 ataagaaatg ggataagtgt ttacaatgta actagaataa aaaatactaa aatggagttt
    13261 catatagata gacaagatat aaaagacttt aagaatgttt atagaggaag taagtttgat
    13321 ataaaggtaa agcaaaaaac tggggtccct tttattatca aaagagttta taagcacaaa
    13381 ggtatgtgga tttgtgctct tgtttcttta tttttattga tgtctacatc acagtttgtg
    13441 acagatgtat atatacaatc tcctgaaggt ataaaaaaag aagctcttag aaatgagctt
    13501 tataaggttg gagtaagacc aggagtctat aagaagagta ttgatagaaa agaagttaga
    13561 gaccatatga tgtcaaaatt taatgatgtg gcatatttat ctatcaatgt aaaaggaaca
    13621 aatatatttg ttactgttac aaaaaaggca gaatccctaa aatctacaga ccaatcaaat
    13681 tattgtaatg taattgcttt aaaaaatggt ataatagaaa aggtaatccc tagaagtggt
    13741 aaatcagtta ttaaatctgg agacatagtc caaaagggag atgtcttatt aaatggagct
    13801 aataccaagt caataccaga agtatgggca agtacttttt atgagtccac aaaaaaggca
    13861 agttatgtag atactgtaaa taaaaaaact ggagaaaaga aaaatatata tacactaagt
    13921 ttttatgata aagagtttac aatgagaaaa aatataaaat ataaagatta tgttgttgaa
    13981 aataaagaaa aaaaactatc aataggaaac tatacttttc caataaagat aaaaacgagt
    14041 actttttatg aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt aaaaaaagaa
    14101 ttatctgaaa aagctttaaa agagttagaa tacataatcc cagcttcggc tagaataata
    14161 gatgtaaaac ataactttaa ggttaataaa aatatgttag aatatttaat aacagttcaa
    14221 actagtgaaa atattgctaa aatttatcct cttagtaagt cagaagcaga aaggtttata
    14281 aaggaagaaa gtaaaccaga tgaaggtgaa gaggaagttc cttccaatcc agaaaaaaga
    14341 ccattaaatg atataagaaa tgagtttgat gaggataata aggataataa agatcaaaac
    14401 aattctgatg aaaataatag taatcaaaat agtaataata gtcaaaataa taatagcaat
    14461 aacaactaat aaaattaaac ttaaaatctt aggaggacta attcaattga taatacaaaa
    14521 gaaatttaca atagcagatg gtaactttga aagagagtta tttggcaact ttgatgaaaa
    14581 tgtaaaatta atagagaaga ctttaaatat agatgtaata cttagagaag gcaatataat
    14641 tttaattgga gaagaaaaaa atgtagattc tgcattaaaa cttatgaatg aattacacca
    14701 aactgtatca aatggtaagc atttagataa gcagagtatt tcttattcat tatctctttt
    14761 gcttgaagga agtgaacaaa aaataaaaga acttgagggg acaatcgtca taactcaaag
    14821 gggaaaagct gttcaaccta aaaccttagg tcaaaaagaa tatataaaac ttatagaaaa
    14881 taatgatata acttttggtg ttggacctgc tggaacagga aagacctatc ttgcggttgc
    14941 tatggcagta aaagctttta aaagagatga agttagtaga attatactta caagaccggc
    15001 tgtggaagca ggagagagtc taggtttttt accaggagat ttaaaggaca aagttgaccc
    15061 atatttaaga ccactatatg atgccttatt tgaaatgttg ggtgctgata aatttaataa
    15121 gtatttagaa agaggaacta ttgaggttgc accacttgca tttatgagag gtagaacact
    15181 tgataattca tttataatat tggatgaagc tcaaaatact acttctgaac aaatgaaaat
    15241 gtttttaact agacttggat ttggctctaa agctgtagtt acaggagatg taactcaaac
    15301 agatttacca caaaataaga agagtggtct aatacaagct acagagattc ttaaaggtgt
    15361 tccaggtatt gggtctataa tgctaacaga tagagatgtt gtaagacatg agttggtaca
    15421 gagaataata agagcttatg agaagcacga caaaagagaa gaatttaaaa aagaagaacg
    15481 taaaaaagcg aaaatgcagg aaaagacttt caaaagaaag taaaagtgta gtaatataga
    15541 tatatagcat tattaatgaa taaaaagtag gtgtagtgaa tggatttgat actagatgat
    15601 agacaagata aattagaagt aagtgaggag ttaattgaaa agataaagga tataatcata
    15661 gaatgtttag attatgaagg atatgatgat aattatgaag tgagtctttc ttttgtagat
    15721 aataaagaga ttcatgaatt aaatagagaa tatagaggag ttgatagagt gactgatgtt
    15781 ttatcatttc ctcttttaag tgatgatttt gaggatgttg aattagaaga agaatcccta
    15841 ggtgacatag tagtatctct tgaaagagcc ttagaacaga gtatagagta taaccatagc
    15901 tttgaaagag aagtatgttt tttaatatgt catagtatgt ttcatctttt aggttatgac
    15961 cacgatacag atgaaaatac aaaagaaatg agagaaaaag aagaacacat attaaataag
    16021 ttaaacataa caagggagta gtttttatga aaccagaaaa aactcgtcaa ggtataatta
    16081 aagcatttaa tgcagctata gaagggatat tgtatacatt taaatttgaa agaaatatga
    16141 aaatacatta tttaggttca gttgctgttc ttataataag tttgtttttt aacttttcaa
    16201 aattagaaat gataatgtta cttatgtcca tatgcttagt tgtagttgct gaaatgttta
    16261 atacagctat agaaaaagct gttgatttag taactgatga atatcatgtt cttgctaaga
    16321 tagcaaaaga tgttgcagct ggaggcgttc tagtggcagc actaaattca gtagtagttg
    16381 gatatatttt gttttatgat aaattgacag atatatctgg aatattaatt tataaaataa
    16441 gagaatcaga attacatata accttgatat gtatattatt ggttcttata gctgttgtag
    16501 ttgtaaaagc attgacttct acaggtactc ctctaaaagg aggaatgcca agtggtcatg
    16561 ctgccttagc atttgctata gcaactgcaa ttacacttat gactgaaaga gttgttgcct
    16621 ctactcttgc atatataatg gctgtattag ttgcccaaag tagaattgaa ggaaaaatac
    16681 ataccttttg ggagactatt gctggagctt tgttaggtgt gttgatagct atattagtat
    16741 ttcagcttgg aatgttctat aattaaaata aatgtacata aaatatttaa aataaaacaa
    16801 atctgtgcta attaaatttt tatgatataa tgtagagtat taaataaaaa taacttttaa
    16861 ttattgtata caggaggagt tgttgtggat aacatagaat tgttaaggtt ggcagaagat
    16921 gccagacaac attcttatgc tccttactca ggttttagag taggtgcagc tcttttaact
    16981 aagagtggta aagtttatac tggatgtaat atagaatgtg catcattagg tggaacaaat
    17041 tgtgcagaaa gaacagctat ttttaaagca atatcagagg gtgataagga tatctataaa
    17101 atagctatag caagtgataa ctcagaaaat aatgagcaaa catatccatg tggaatatgc
    17161 aggcaggtaa ttatagagtt tggttctgat ataaaaataa taacgggata tactaaagga
    17221 gaggtgtttg agcatagtat aaaggatttg ttgccaaact atttctctgg aaatgattta
    17281 aaataaattt aaaagtataa ataaatttaa gtaattaaaa caagaagata aaaagttagg
    17341 agacagttaa tgttcaaatc aggttttgtc agtatagtgg gtagaccaaa tgtaggaaaa
    17401 tctactttaa tgaataatgt agttggagaa aaaatagcta taatgagtga taagcctcaa
    17461 actacaagaa atacaataca agcagtatat acagatgaag aaatgcagat agtattctta
    17521 gatacaccag gtattcacaa accaaaaaat aaattaggtg aatttatggt aaaggcagca
    17581 acagaagctt ttaaaaatgt agatttaata ttatttgtag ttgatgattc taaaaaaata
    17641 ggtcctggag atagaaagat aatagaggat ttaagaagtg ttaagacacc aataatttta
    17701 gttgtaaaca aaatagacca actagaccaa aaagatgagc tatttgatat aatcaaaatg
    17761 tatgatagag aaggcatttt taaagaaata gtaccaatat cagcattaaa aggaaagaat
    17821 acggatacct taataaaagt tatacaaaat tatttagaag aagggccaaa gtattttcca
    17881 gattatatga ttacagacca gccagaaaga gtattaatag cagaacttat aagagaaaaa
    17941 gttcttcact atcttaatga tgaaatacct catggtgtag ctgttgaaat tgaaaaaatg
    18001 aaagcgagaa atgataagga aatagtagat gtatctgctg taatatactg tgaaagagat
    18061 tcacataagg gaataataat aggaaaaaat ggtaggaaac taaaaggaat aggtaagtca
    18121 gcaagacaag atatagaatt gcttttaggt tcacaaataa atcttcaatt atgggttaaa
    18181 gtcaaagaaa attggagaaa tttacaaaat tatataaata attttggata taatgataaa
    18241 taaaggtggg ttatatggat aggaaactgg atgcttccca agatttatta tatgaagtaa
    18301 aatcattaat tgataataat aaagtattgg agttaagaga attaatagag gaatatcata
    18361 taattgatat attcgatata atggaaaatt tagaggaaga catgaaaatt caattatttg
    18421 aagtacttcc tttagatatg gcatcttcta tattagaaga aggtagtgta gagtttttta
    18481 ttagtatttt atctaaactg gatgtagaac actcgaaaaa tatacttgaa cttatgtctc
    18541 ttggagatat ggcagacaag ttgagtgaac ttgaagaaga agaaagagaa cacataataa
    18601 atcttttgaa tcaagagaat gccgattatg taaaagagtt actattctat gatgaagatt
    18661 ctgctggtgg aacaatgacc actggatata tttctataaa taaagatatg acagctcttg
    18721 aggctataga ccatatgaga gaagaagctg aagaagctga aactatttat tatatatatg
    18781 tggttgatga tgaagaaaaa ctagttggag tattgtcatt aagagaatta ataatagcta
    18841 gagacgcaaa tatagtagaa gatttgatga gtgaaaatat aatctctgtt tatgtagatg
    18901 aagatagaga agaagcagta agattagtat ctaaatacaa tttaatagca attccagtag
    18961 tagacagaca agaaaaatta aaaggaataa taacagtaga tgatataatt gatgtaatgg
    19021 aagaagaagc aactgaagat atgtataaat ttgcaggaag ttcagaacat gaaagagaag
    19081 ttgctgaaaa agaaaaccct acattgagag agcaaataat atcagcactt agaggacgat
    19141 taccttggtt aattattacc ttagttggag ggttgttggc ttcgttaata ctttctaact
    19201 tagactatat aatgaatcct gtatatgctc cattagtatt ttttatacct gtagtaattg
    19261 gtatgggtgg aaatattgga actcaatcat catctgttac agtaataaca ttgtctaata
    19321 aggatttaaa ttttagcaat gtggttagag aaggtattgt aggtattatt acaggtcttt
    19381 tgtgtagtat aataacaggt attgtaattt actttgttat gagaaagtta gacattgtat
    19441 taattgtttc aatatcatta tttataaata tggttttagg tgcaacaata ggtgcgttta
    19501 tgcctgtttt attaaaaaaa atggatgcag acccatctac agtttcatca ccaattatat
    19561 ctacagctct tgatataact ggtatagcag tttactttat aataacaacg gcattattgt
    19621 caaaaattgt ttaataactt tatgtatgat ttatcctctt cttactaaaa atataagtat
    19681 aaatgtaaga agggggtttt ttaaaaatga acaatatcag ttgggaagtt tttaaaaaaa
    19741 caggtagtat agatgcatat ttatatttct gtgattgcaa aaacatacat gaagaaattg
    19801 aagaagtaag ggagaaaaaa gaagacgatg ataatcctga acacccaggg gatagtactt
    19861 aaagctataa ggtataagga aagtgatata atacttacac tatttacaag aaagcttgga
    19921 aaagtatctg caatagcaaa aggagctaaa aaaaataaga gctctttgct ttcatcatca
    19981 cagttatttt cttatagcaa ttttacctta aaaaaacaag gtaatatgta caaggttact
    20041 caaagtgaaa taataaaaag tttttataat atatcctatg atatagaggc attttcttat
    20101 gctacatata ttacaaaatt agttgaaaat tctatacttg aaaatcagac taataatagg
    20161 ctatttatat tacttgcgca gactctgtat ttgtatacac aggataatac agacaatagg
    20221 tttataacag cagcattcga gttaaaattt ttagactata taggatttaa acctatagta
    20281 aataaatgca ttaattgtga atgtaaaatt ttacaaaatt ctgtgtttaa tatctacgaa
    20341 ggtggtatac tatgtagtaa gtgtagtaaa ctttttgata ataatataaa attagactta
    20401 acaactataa gtcttatgga atatgtatta agaaatgata ttttaacatg tagtaaagct
    20461 aaagtatcaa aatatataac acatgaactt gagaatattc tagacaagta tttgaaagtt
    20521 tatgttgata atattaattt taaatcatta cacgtattgc aaagtgtaaa aaataataag
    20581 ggagtggata atgatgagta gtaatataac tattgaaatg gttgattcag tctttgaaag
    20641 agttccagga gcaacttatg cagaagcaaa agaagcatta gtaatgtgca atggagatgt
    20701 aattgaggca gtaatatatt tagaaaataa aaaaaatact tgtaattgta aagcaaaatc
    20761 tgctaaagaa actatggaag aagtatttgg caaagatgga gaaaatataa agtttcaatt
    20821 aaaagagctt cttagaaaaa gtagtgtagt gagaataata gttgaaaaag atggaaaaac
    20881 tatgatgaat attcctttaa cagttggagt tgttggatta gcttttgtac cattagctac
    20941 tttagttgga ttatcagctg ctgttataag taaatatcgt ataaaagttc aaaatgaaga
    21001 agatggagaa attgtagatt taggtgaatt gaatgaagaa aaacttcacg ttttgaagaa
    21061 tatgataact aatgcagcaa aagatgtgaa ggatgtagta gttgacaaaa aagaagatga
    21121 caaagatgta actgctgacc ttatgaaaga agaatctgaa aagaataaag aagacgaaga
    21181 cgaaaataaa tagatataaa gtgactttta aaaaatgaag agaagataat tataagaatt
    21241 atcactaaca ttgacaaatt ttatagaatt tgatattcta aaagttgtag aagaactatt
    21301 aaaatagcct ttcgtgtaga cgagaggctt tattgttata ggagggattt ttatgaattt
    21361 tcaagagatg atactggcgt tgcaaaaata ctggtcaaag caaggttgta tcatgatgca
    21421 gccttatgat attgaaaaag gtgcaggaac tatgaatcct aatacttttt taagatcatt
    21481 aggacctgag ccttggcaag tatgctatgt agagccttca agaagaccag cagatggaag
    21541 atatggtgaa aacccaaaca gactatatca acatcatcaa ttccaagtta tactaaaacc
    21601 ttctccagat aatatacaag aattatattt ggaaagttta aaagagattg gaattgaccc
    21661 aagtgagcat gacataagat ttgttgaaga taactgggaa gctgctacag ttggtgcttg
    21721 gggacttgga tgggaagttt ggctagatgg tatggaaata actcaattta cgtattttca
    21781 acaagtaggt aatattgaat gtgagcttga gacaggagaa attacatatg gattagaaag
    21841 attagctatg tacattcaag aagttgatag tgtttatgat ttaaaatgga atgataaaat
    21901 aacttatggt gaagtattta ataaagctga gtatgaaaac tcaatgtacg catttgaatt
    21961 atgtgatgca gatatgttgt ttaatctgtt tgatatatat gaaaaagaag cattaagact
    22021 tatggaaaat ggtcttgtaa taccttcata tgactatgtg ttaaaatgct ctcatgcatt
    22081 taatacattg gatgcaagag gtgcaatagg agttagtcaa agagcttcat ttataggtag
    22141 agttagaaat atggcaaaga ctgttgcaga aacttttgta aaacaaagag aagaaatggg
    22201 attccctctt ttaaaggacg gtgacaaata atgaaaaatt atcttttatt tgaaatcggt
    22261 gttgaagaaa tgccatcaag atttgtaggc agtacactag aacaattaaa aaacaatctt
    22321 agtaaattat ttgatgaaaa tagaatagct tttgataaaa taaatgcgta tgggacacca
    22381 agaagactta ctttagtagt cgaaggattg agtgatagac aaaatgattt agaagaggaa
    22441 ataaagggtc ctgctaagaa aattgctgtt gatgcagaaa ataatttaac aaaaccagca
    22501 ttaggattta taaaaagcaa aggattaagc gaatctgaca tatattttaa acaagttgga
    22561 aaagaagaat atatatttgc gactataaag caagatggaa aagaaacttc agaagtacta
    22621 aaagacatat taccagagac tataaaatct gtagtattcc caaaagctat gcgttgggga
    22681 ggaaaaaatc ttaaatttgc aagacctata agatggatag ttgcattgtt aaatgataaa
    22741 gtattagagt ttgatttaga aggaataata tcttctaatg taacaaaagg tcatagattt
    22801 ttaggtgaaa gtacaataga agttgattct atagaagatt attttgaaaa attagaaaaa
    22861 aattatatag ttttagacca aaacaagaga aaagaaatga taagaaaaca atgtatagaa
    22921 gtagcaaatt ctttaggtgg agaagttgaa tttgatgaag atttacttga agaggtaact
    22981 tatttagttg aatatccaac tgctttttat ggagaatttg atgtggatta tataaaacta
    23041 ccaaaagaag ttgttataac tcctatgaaa caacatcaaa gatattttcc agtacttaaa
    23101 gaaggaaaac tgttacctaa ctttatagct gttagaaatg gtgattctta cagaatagac
    23161 aatgttaaag ctggaaatga aaaagtttta gaagcaagac ttgcagatgc tttatttttc
    23221 tatagagaag atactaagag aaacttagaa agctacatag aaaaattaaa aactgttgta
    23281 ttccaagtaa aactaggaac tatttacgac aaaactttaa gacttgaaaa attaagtgct
    23341 gatatacttg atagtttagg tttagtagat gaaaaggcag atactataag ggctgctaaa
    23401 ttatctaagg cagatttagt tactggaatg gtaggagaat ttgatgaatt acaaggtttt
    23461 atgggaaaag aatacgcaaa agttggagga gagaatgatg ttgtaagtga agctattttt
    23521 gaacattatc ttccaagatt tgcaggagat atacttccta agactaatgc aggagttgcc
    23581 ttatcaatag ctgataagtt ggattcaata gcaggtttct ttgctatagg aatacaacca
    23641 acaggttctc aagacccata tgctttaaga agacaagctt taggtatact tagtatactt
    23701 atggacagaa aagttgctgt ggatataaaa gtacttattg aacatgcttt agacaattat
    23761 tctgaattag attttgataa agaagaagtt gttgagcaaa taatgaattt cttcaatgag
    23821 agagtaaaaa atctatttaa agatttagga ataagatatg atgttgttga tgctgtttta
    23881 agttctgata taaatgatgt ttctgatatg catatgagag ctttagagct taataattgg
    23941 ctagaaaaag atgaattagt ggagatgctt actgcattta atagagtttc tactctagct
    24001 caaaaagcag aaagtgataa aattgatgaa tctttattaa aagaagatgc tgaaattaaa
    24061 ctttacaatg aatttaaaca agtaaagata aaagttaatg aattattaaa ggataagaaa
    24121 tatggtattg ctttagatga atttgcatct ttaagaccag tagttgataa tttatttgat
    24181 acagttatgg ttatggataa tgatgaagct attaagaaca atagacttgc tttacttaaa
    24241 caagtatatg atactatgct agaaatttgt gatttatcta aaattgttta taaataaaat
    24301 ttaaactatt tataagaaat aaggctgtct caaaatagag atagtcttat ttttcattgt
    24361 tgagatgatg aaaatatatt tgatttcata gaaaaagagt gtctcataaa ctaaaaagtt
    24421 cattttgaga cacccatgtt ttttgtaagt aaatataata aactatattt tatacttatc
    24481 agttattttg tttaaaatat tatcatttga atttacccat tcataaaaat tgcctgatga
    24541 tgcgtccgat attttataaa gcatttcagg aatatcacta gctttaaaat ctccaagtga
    24601 ttttcccaat ctagtcttac ctatttcaaa actgcctcca acaaataatt caaatgtatc
    24661 tgtagatata ccatcaacat tcttcttttt accacataaa cctatgctac caacttgatg
    24721 aacaccacat gaatttaaac atcctgatat atataactta ggtgctttat ttaaaatttc
    24781 ttcattattt tgtaatctaa aataatctat gatttcatgt aatattttct gactattttg
    24841 tatgcccatt tgacaaattg gaacacctat gcaagatata ctttgttcta attgagaatt
    24901 acaactaata gttttactta tttctaatat tctttttgct tcatttccat ttaaatttaa
    24961 aatatataat ccctctgtca ttcctagtct aatcatagga ttttcaacat tatctaattc
    25021 tttaagtaaa gtacttaaat cctttgttaa caaaatacct ccaactggat gtatatatac
    25081 agtgtataaa ccatcctgtt tttgaggtat tagtctagaa tcttgtatat ctattttaac
    25141 tccaggttta gagtaatcta tttcttcaat attgagttca agactaccat tttctttttc
    25201 taatttatag tattctttaa atttttctaa gaatagttct tcaccaagtg attcgaccat
    25261 atatctaacc ctagctctgt ttttattttc ataatttcca taatccataa agaatttaat
    25321 cataccttca acacaatata aggcgtcttt cggctttata agttcatcaa gttctaaagc
    25381 aactttgggt tgtttaccta gacctccacc taagaaaact ctgaaatatg gttcatcatt
    25441 tttcaaagta gcaacaaatc ctaagtcttg tatactacaa tgagcagtat ctgtgtaaca
    25501 attagaataa gaaactttta attttctagg taaatggtat gtagtagcat ttttgataaa
    25561 atatttatca gtagcaactg catatggagt aacatcaaag acttcactag ggtcaactcc
    25621 agataatgga gataatccga catttctagg ataattacca ccaccacctc tagtgaatat
    25681 atcatttttt attccttctt ccatgatatc aacaattgaa tttatagata aatcgtgtaa
    25741 ttgtatagct tgtctagttg ttaagtgtat cttatctaag ttgtgtttaa tagctatttc
    25801 atagatttta ttaagttgat actgagataa aactccagca gatactctaa gtcttatcat
    25861 aaaagatttt ttgtctcttt gagcataaac accatatcca ccagaaaatc ccttatattg
    25921 cattttattt atttctccat ttaaaaattt tgaagaatat tctttaaaat taggtagttc
    25981 atttaacaat atctttttaa aattatccat tatttcacct cattgttagt cttgtataaa
    26041 aatatatcta ttgtataata gatatatata ttattatcta gtcaggataa aaatataaca
    26101 gaataattct attaaaagta agaataataa ggacatagtg agtattttgt ttttatttta
    26161 caaattaagt ataatatttt tgttttataa aatataatta agataaatag ataattttgt
    26221 aattttaata tataatattt agtagagtgt gggagtagca cttaatattt gcaggaggag
    26281 tttttgtata tgggagaaat aataaatgca ttagatagag ccctagacat aattttgctg
    26341 ttatatcatg aaaagagaga aatgggaata actgaaataa gtaaggctat gggagtatat
    26401 aaaagtacta tacatagaac tttggttact cttgaaaaca aaggttttgt tatacaaaat
    26461 gcagagaatg gtaagtattg gctaggaata aatttatatg caataggtat ggttgtggga
    26521 gaaaaaatgt cattgactga aatagtaaaa ccatatacga aaaaattaaa tcaagaattt
    26581 aacgaagtag ttaatgtatc tatattagaa gaaagagctc aagattctcc aagaagtatc
    26641 ataatacata aagagtatgg ttcaaatcaa ttactttcag taaatccatc cgttggttca
    26701 agtagtgaat gttattgttc agcagttggt aaatgtctta tggcatttaa tgattctata
    26761 gattttgaaa aatatagaaa aacaccaata cacaagtata cagaacatac aattgataat
    26821 tgggatgata tgatgctttt tttagcaaaa ataaaagaac aaggatatgc tatagatgat
    26881 gaagaactag aacatggact aacttgtatt ggagcaccta tcttagataa aaataataaa
    26941 gctattgcag ccataagttt gtcaggtcca actataagaa tgagagaagg ggactttgag
    27001 tataagatta aaagagttat agaaactgca aaaagtatat ctgaactttt tagatgattt
    27061 ttaatctcga taataaaaat aggctatatt taaattgtta tttgtaaata tcagtttaaa
    27121 tgtagcctat ttttctttaa tctaaaatta aatattctta aaatttaaaa tgttattgaa
    27181 atatataagc gaatgtgtta tcttttaatt aagcaataaa aatagaacaa ggttctatac
    27241 tatagaacaa aattgatgca tatatagtat ttatacaaaa tataaatatt atatatttaa
    27301 aattaagtaa aaatattaca taaaggggag atttgagaaa tggaaaaagt caagttgagt
    27361 gttgaaagat gtaagggatg ctatctttgt attgaagctt gcaaaaaaga tgcaataagt
    27421 ctttctgact acacaaataa aaaaggcttt gtaactatta aagtcgatga agaaaaatgt
    27481 attcaatgtg gcgcttgtta tacaatgtgc cctgatttag tatttgagat attataaatt
    27541 atttttatta gaggggagaa aaggtatgca aagtttatta attaaaggaa atgaagcttt
    27601 agcagaaggt gctatacgtg gtggttgcag attttatgca ggttatccaa taacgccaca
    27661 atcagaaata ttagaaatga tggcatggag acaaaaagaa gttggaggag tatttatcca
    27721 aggtgaatca gaaatagcca gtgtaaatat ggttatggga gcaagtgcac ttggagcaag
    27781 agctatgact tcttcatctg gtccaggatt ttcattaaaa caagaaggta ttgcatattg
    27841 ggtatcagct ggtttgccag cagttgtagc atgtgtgcaa agatatggta taggtgatgg
    27901 atttatagga gctggtcaag ataattattg gcaagcagta aaaggcggtg gaaatggaga
    27961 ttatcattta atcgtgttag cacctaatag tgttcaagaa aatatggata tggcatatga
    28021 atcttttgaa ttggctgaaa aatatatgca tccagtactt atactttctg atgctacaat
    28081 aggtcaaatg gtagaacctt gtatagcacc accaatgaaa gaatacgata tggataaagc
    28141 tccatgggct gcaaaaggaa cacctgtagg agaaaaaaat aaagtaataa ctgatataac
    28201 atattttgaa gaccaagata catggcaaaa agggtatata gaaaaaatga gaaaagttca
    28261 agaagatgaa caaagatggg aagagataga aatagaagat gctgaagtgg tatttgtggc
    28321 atacggtatt tcatcaagaa tagcagaagg tgcagttaga gatgctagag caaaaggaat
    28381 gaaattaggt ctaataagac caaaaacatt gtggccttac ccaagaaaag catttaagaa
    28441 attaagtgat tcattgaagg gacttgtaac agttgaaatg aacatgatgg cacagatgaa
    28501 ggaagatgta attatagcat gtgataatag atttccagta tattcagtag caacagctca
    28561 atatatggca gaagtaaatc aattaataga ttttgctcaa cagattatag atggaaaagc
    28621 taagcaagag gaggtatttt aattatggct aaaaaagcac cagcaataat gatgtgtgaa
    28681 aatatgttct gtggaggctg tggacactct atagtcgata gaattattgg agaagtatta
    28741 acagaaatgg atatatgtca agatacaatt atatgttcag atataggttg taatcatatg
    28801 tttcaattta gtatgaatgt agatgttgtc gttccacctc atggtaaaat gggagcagca
    28861 atggcagcta tgaaaagagt tagaccagat aaatatatat ttacaatagc tggagatggt
    28921 ggagcttatg caatagggct tggagaaact atgtcagctg ctactagaaa tgataatgta
    28981 atatttcttg ttatgaacaa taatatgttt gcaatgacag gagggcaaat ggctccaact
    29041 actatagaag gtcaatatac agcttctact ccagcgggta gaaattacgc agatcatggt
    29101 tcaacatttg atgttgtaaa agttatggga aatttagata tagcatatct agcaagagga
    29161 acaataacta accctaaaga aataaataca acaaaaagat acatcaaaaa agctctagaa
    29221 aaacaaaaag aaaagaaagg tttttgctta atagaagtgc ttgtaccatg tccaacaaac
    29281 tggggattaa agccagtaga tgctatgaag agaattgaaa atgaacttgt tccaaagtat
    29341 ccacttggag aatttattga ttagggagga aataatatgt tgaatgaaat tatatgtgct
    29401 ggttttggag gacagggagt tcttacagca ggaaaaatta taatgtatgt tgcatataag
    29461 gcagaaaaga aaattacatg gttcccatct tatggaaatg agatgagggg aggaagtgca
    29521 aattgcaatg ttgttgtaag tgatgaaaaa attgcaaatc cttgtgtaaa aaatccagat
    29581 atactattgg gaatgaacag cgtatcagta gataagtatg aaagtttaat gaaaaaaaat
    29641 ggttatatgt ttgtcaatga atcactagta agtaaggata gaaagtacag agaagacatt
    29701 aatgttataa aagtacctgt tactgaaata gcacaaaatt taggcaatga aaaagctgct
    29761 aacatttgca tgttaggtgc tatggtaaag agtttggatt tatttacaaa agaagaattt
    29821 gaaagtggta tgtgtgaata ctttgaaaat caaggcaaag gtaaattcaa ttctaaaaat
    29881 gtagaagcat ttgaagctgg atacgactac gaatgaatat tttaaatatt taaaataatt
    29941 attgaaatac ttatttttat atgttagtat ttaattaata aaatatagaa cgtcgttctg
    30001 gacaatggga cgaagtcttt gggtcggaat acaagaacgg cgttctgtat aatagaacta
    30061 gggggtgtta aattgaaagt tttagtaata aatcctggtg gtacatccac aaaaatttct
    30121 gttttccaag atgaaaatga aattttgaaa aaaaatataa cccatacaag agaagattta
    30181 aaaaattttt ctaaagtatt tgatgaatat gattatagaa aacaactgat agtagatatt
    30241 ttaagtagtg agaatcatag tataaattct tttgacgcag tagttggtcg tggtggactt
    30301 atgaaggcta taaaaggtgg tacctatact gtgagtgaag aaatgattga ggatatgaga
    30361 aatgaaataa atggggaaca tgcatcaaat ttaggtgcat tattagctaa aactatagct
    30421 gatgaaatag gagtacaatc atttgtagta gacccagttt ctgtagatga atttgatgat
    30481 gtatcgagga ttacaggaat atcagacata gaaaaagcaa gttggttaca tgcattaaac
    30541 cataaagctg tttcaagaaa aattgcacaa aaactagggg gaaaatacga ggattataac
    30601 tttattgtag ctcatttagg ttcaggaata tcgattgtag cacataaaca aggcaaaatg
    30661 attgatggaa gtggtggtag gtctgatggt ccattttctc cagaaaggag tggtggatta
    30721 cttgcatatc ctcttataga attgtgttac tccggaaaat atactcatga tgaaatggtc
    30781 gcaaaagtaa gttcgattgg gggaatgtac gattatttag gaacaaagga catgatagaa
    30841 attgaaaaca gaatcaagga gggtgatgaa aaggcaaaac ttataatgga tgcatttatt
    30901 tatcaagtag caaaagaaat ttctatgtat ggagcttcac tttcagggaa tgtagataga
    30961 gttatcttga caggagggat atctcactca gaaatggtag tcaaaggagt agcagaaaaa
    31021 gtttcatatc tagctccaat agagatagta ccaggagaga tggaaatgga ggcacttgct
    31081 ctaggggcac ttagagtact taatggagaa gaaatagcaa aaacttatta atatttagga
    31141 gaaagagtat gttaaaagat tttaataatt tattagaaga ggctaaaaag cttgaacctg
    31201 ttattgtaag tgttgctgtt gcagaagaca aagaagtatt aaaggctgtg aaatcagcaa
    31261 cagatttaaa cattataaag ccaattcttg taggagacaa ggataaaata gaaagtatat
    31321 caagagaaat tggattaaaa ggtcatgaaa taataggttg tcaaaatccc gatgaatcag
    31381 ttaaaaaagc tgtagagata gttaaaaata aggaagctaa agttcttatg aagggattag
    31441 taaatacaag tgtctatatg agagctatat taaataaaga aacgggtctt agaacaggaa
    31501 gattattaag tttgcttgca gtatatgaat tacctcaata tcataaactt ctttattgta
    31561 ctgatagtgg aataaatgta tcgccaaact taaatcaaaa aaaagatata atgacaaatg
    31621 cacttcttac tatgaaaagt ataggcatgg aaaatcctaa aactgcgata cttactgcaa
    31681 atgaaatggt agaccctaag atacaatcta cagtggatgc caacctactt gtagagatgg
    31741 aaaaatcggg agaaatacca aagtgtatag tggaaggtcc aatagcattt gatgtagcat
    31801 ttgatgcaca tgcagcagaa cataaaggca ttaaaagtaa tattgctggt gatgttgatt
    31861 tattagtatt tccaaatata gaagcaggga atatattagg aaagtcatgg cttagattta
    31921 ataaagctaa gtgggcaggt atagttttag gagcaacaaa tccgataatt ttaggttcac
    31981 gttctgacac agctgaaata aaaataaatt caatagcatt agcgtgccta gcatcacaat
    32041 aagaaattat tattaaggag gaaaaagtat tatgaaatat gcaaaaagaa tggatatggt
    32101 aaaagcttca gctataagag attctcaaaa gaaaatagct cagaaggtag caagtggtgg
    32161 aactgttata tcttttgctg cagggcttcc tgacccaaat ttatttccaa tagaagagat
    32221 aagtgaaata actcaaaaag tacttaatga aaggggaaaa tttgccctag catatggacc
    32281 tactaaaggt gaagatgagt tgttagatct attagtaaaa agaatgaaag aagaagaaaa
    32341 tattgataca aaagcagaaa atattataat aactactggg tcacaacaag ggatagcatt
    32401 aagtgctatg attttattag aaaagggaga tgtagtagtt acagaaaatc caagttactt
    32461 aggtgcaata aatgcattta gaccttatga atgtgatttt ttaggagtag atacagatga
    32521 agaaggaatg gtaacagatg atttagacaa attattatct gaaaattcta acataaaggt
    32581 gatatatgta ataccaacat ttcaaaaccc tacaggaaaa acatggtctt tacaaagaag
    32641 aaaagatttt atggaagtcg tgaataaata tgatgtcata gtaatagagg ataatcctta
    32701 tggagaaata agatttacat cagaacctct acctacactt aaatctcttg atactaaaga
    32761 taaagtattg taccttgggt cattttcaaa ggtattatgt ccaggattta gagttgcttg
    32821 gatgtgtggt aatgtagaca tcatagataa ggcagaactt ttaaaacaag gtatagattt
    32881 acagtcaaat caattttcgc aagtacaagt aatagaattt ttaaagaaat acaacttaaa
    32941 tgaacatata gaaaaaataa gagcagagta taagaaaaga tgtctgctaa tgttagatgc
    33001 tatgaagaaa catttcccag aggaagctaa atatacagaa ccagatggag gaatgtttat
    33061 atgggttgaa cttcctgaaa gtataaatgt agatgattta ttagataaag ccattgatgc
    33121 tggagttgct tatgtttctg gagagtcctt ctttgcaaat aatggtccaa aaaatactat
    33181 gagacttaac tatacaacaa tgtcagaaga acaaatcgtt aaaggtgttg agatacttgc
    33241 agaagttata aaaaaagaac tcaattatgc ttgtgaataa atataaaaag tgagggttta
    33301 aatatgggag aaaaagaaaa cggtataaag tttggaaagt taatgtttca acttgttatt
    33361 atagcaatat cttgggaatt agtttatatt ataccattta tccaatatac attatatgat
    33421 ccaatcctaa aagcattaga atgtacaaac actcaactag gttttctact tacaatttat
    33481 gggttaggta atatatttgg agctcctata ggaggctggt tagcagatag atttgattat
    33541 agaaagataa ttttaggttc tgtatttctt aatggagtag tatcattttt atttgcattt
    33601 aatatgaact atccatttgc agttgctact tgggtaggtt gtgcaatcac atcattagtt
    33661 atgaactatc cttctatggt aaaaatactt agagttatag gaaaagataa tcaaggtaag
    33721 gtttatggat ttaatgaagc tatggttgga gtttctggag tagtaatggg agcaatattc
    33781 ttatatattt atacatgctt tgcaactcct acattgggta tgagatgggt aatgatatct
    33841 ttaggaatat tatcaatagc aatgtgtcca gtattatggt ttgtaattaa agatgtagat
    33901 attaaagaag aaagagaaga agcaggtgaa aaaatgtctg ctggtgattt tataacagta
    33961 ctaaaatcac caaatacttg gctagtagga ataagtatat tctgtgtata ttcattcaca
    34021 gttacaatgt cttattttac accatatata acatctgtac ttggaggttc tgtagcatta
    34081 tctggtgcat tagcaataat aagacagcat ggtctaaaat tatttggagc accatttgga
    34141 ggatattgtg cagacaaatt aaaatctcct acaaaagtac ttttaccaat atatgtattt
    34201 ggtatagcag taatagtttt attcctagta ttacctgcat caactccaat gactatattc
    34261 atagctttaa catttgtagt aggtatatta ggttacatgg gaaaaggtat atattatgca
    34321 gtacaagatg aagtaaaagt tccagttaaa tattcagcaa ctactatagg tatagctgca
    34381 gctttaggat tttcaccaga tgtattccaa tttgcattaa taggacactg gatagatact
    34441 tatggcaata aaggttacac atatacattt atattccaaa tagcaattct agttattgga
    34501 atactatctt gtttatatat attaaaggtt aaaaagagaa gagcaagtaa attagaagct
    34561 taaatattca atttggtaat taagtatata taaatacaat acttattttg atgaatgtga
    34621 ttgtataaat aacataaaaa atatcaatag attaagtttt ataataatgt ttagagaata
    34681 aagtataaaa atcaaaatgt tatcaagttt tctaggtttt aacataaagt tacccctgaa
    34741 ggtgtaaaat tgagtggcaa ttattatcta ttttcttacc tacataaacc tatttaattt
    34801 aaattctaag agattttaaa aatagatggt agttgtactc aattttaaac ctcataataa
    34861 atacgaaaat ttaaaatcta actagatact atttaattat atattaataa atatatattg
    34921 tactatttat ataataataa tttcaggctt ttcattatat gtattccaat ttatattgtt
    34981 tgattattat tcaaacacac gtcgttaaaa attttataaa tttatgttta gatatgatta
    35041 atactcttga ttttagaaat aaatatttgt agattgtaga ttaaagataa tataaataaa
    35101 tagaaggtag attttttaga tatattaata taaagattat ataaaatatt gaaatatagc
    35161 tgtttaaatt attgtctata aatataaaat gaatggtggt gagttaagtg accgatttag
    35221 ttaacaccat taatggaatt gtttggtcac ccgtattagt tgcaatgtgt ttgtgtatag
    35281 gattatattt ttcattgaaa ttaaaatttt ttcaaataag agatgtaaaa gaaatgttta
    35341 ctctcttgct tgaaggagat agttctcatg ttggtatatc atcatttcaa ggttttgcta
    35401 catctatggc tggaagaata gggactggaa atatagctgg tgtagctgta gcaatagcaa
    35461 tgggaggtcc aggagcatta gtatggatgt gtataatggc tacattaggt tcagcaactg
    35521 catttgtaga atctactcta gctcaaatat ataaagatga gcatgatggg cagtatagag
    35581 gaggacctcc atattatttt gaaaaaggtc ttgggtggaa aaagtgttca gttttatttt
    35641 caatagttgc aataataagt tatacatttt ttatgcctgg tacacaatca aacactttat
    35701 atctagcaat gaatcaagca tttggatttt caaaaatgac aatagcagta gcatcttcta
    35761 ttgcattagc gataataatt tttggaggaa ttaaaaggat tggtttattt gctgaaaaag
    35821 tagtacctat aatgggtgga gcatacctta ttatgacatt agtaataatt ataacaaata
    35881 ttagtttagt tccagaggct attgtaacaa tgtttaaatc tgcatttgga tttggttcag
    35941 tttttggagg aatgctaggt tcagcaatat cttggggagt aaaaagaggt atatactcaa
    36001 atgaagcagg agaaggttct gggacttatg gtgcggcagc agcagaagtg agtcatccag
    36061 ccaaacaagg attagttcaa gcattttctg tttatgtaga cacattatta atatgtatgg
    36121 ctacaggtat tatgattgta ataacaggaa tgtataatgt tacaggtgtt tgcacagcac
    36181 tgccaggtgt agaaccggga ccacaatatg tacagagtgc aataaataca ttacatccac
    36241 aattaggtga tatttttata gcaatagttt tattattatt tacatttaca actttattag
    36301 ccttctcata tatgatggag actaatgtat cttacttagt aactagaatt aaagcagaaa
    36361 aacatgaaaa gtacataatt aatgtctgta gatttatgct tatagcatgt gcagcaatgt
    36421 gttgttttat agaaccactt acaagctggg cattaggtga tatatgtata ggtgtattaa
    36481 catatataaa tttaattgcc atctttacac taaaaaaacc agctataaag gcatattatg
    36541 attatataag acagaagaag atgggaataa aaaggagtga tagaactttt aaccctatag
    36601 agttagatat aagaaatgct gagttttggg aaaatagata cttaaaagag gataagcatg
    36661 agttaaatta aagttataat aaaatataaa aattactaaa taaatttagt gggaggtaag
    36721 attggtatga aagaaagaat aatacaagtg gctgattcaa agaaagaaaa aattctaggg
    36781 ctatgtcaat ctttatatga tgaaccagaa attgctttgc aagaatataa atctgctaaa
    36841 aaaatatcag aatttttaag agaagagggt tttgatgttg aggaaaactt ggcaggtatg
    36901 gctacagctt ttaaagcaac caagaaaaat ggagatggtc ctaaaatagc atttatagca
    36961 gaatatgatg ctcttccagg caatggacat gcatgtggac atcatttaat tgcatctatg
    37021 ggagttggag ctggaatagc tctttcaagt atactggata cttacaaggg agaagtaagt
    37081 ataattggaa cacctgctga agaaactgga gatggaaagc cttacttgat agaacatggt
    37141 gtatttgatg gctatgatgc agcaatgatg atacatccaa attcaaaaac ttgtgtaaca
    37201 cctgaaatta tagcaatagg aggtctagat ttcatattta ctggaaaagc atctcatgca
    37261 ggagctaagc catataacgg aataaatgct ttagatgcag tagtattact atataataat
    37321 ataaatgcac taagacaaca attagtagat ggaacgagaa tacatggtat tatactagaa
    37381 gcaggaactg ctgcaaatgt aatacctgat atgggaaaag taagattaga gataagggca
    37441 aaagaacaaa attactttga tgaagtagta gaaaaagtga aaaattgtgc tagaggtgct
    37501 gcaatagcca ctggatgtga acttgaattt tatcactttg aaccaacttg ccaaggatta
    37561 aatgaaaata aagttttggt agacatattt acaaagatta tggaggaatt tggaatatat
    37621 gaagatgaac aagtaatgtt aggttcaaca gatatgggaa atttaagtca aataatgcca
    37681 tgtatacatc ctttgatgaa gttttctgaa aatggagaag aactacatac aaaagaattt
    37741 ttggaggctt ctataaattc atatgcaaaa gatagagtaa ttgatggtat aaaaatattg
    37801 gctttaacag gatttaattt atttgaaaat ccagagttat tgaaaaaaat gaaggaagag
    37861 tagaacttag actattttta attatactct ttaaaataag aagagtgtct caaaatgaac
    37921 tttttagttt atgagacact cttttttgta tgaaattaaa tatattttta ttattttaac
    37981 aatgaaaaag aggcttatct gtttttatat gacctttttt aacttgtagc acaaaatatc
    38041 atgaatgatt acaattcgaa tgataaaatc gattataaga tatataggag gggtacatat
    38101 ggaaacttta gaagtaagaa atattgaatt gagtggtaca aattatgaga taggatacag
    38161 actaggagaa ttggttgcta atatgccaga gataatagaa ggtcagatta ataaatctaa
    38221 tctggtaagc aaaaaagaag aaaaagagat gatagagttg tttgataaat attgtccagg
    38281 tcttaatgaa gaattgcaag gttttgctga tgcaattcaa gtgaattgta atcaaatttt
    38341 atattatact atgacttatc ttaaaccagg ttgtagtcaa gttgcattag caccagaatt
    38401 aactgaaaat gggcatgtac tatttgcacg taatttcgat ttctcacaca atatggagga
    38461 ttttgtttta tgtaaaacta aggtaaatgg aaaatatgca catataggta ctaccgttat
    38521 gcaatttgga agaggtgaag gtatgaatga atgtggctta ggtgtaagtc agtcatcatg
    38581 tggtattcct gtaggcaatt cagatggttt aagaaaacca gcaattgtag gtttacagtt
    38641 ttgggctgtt attagatatt tgttagaaaa ttgcaaagat gtagatgaag cattagaata
    38701 tttaaaagat atgccaattg catacaatat taatttattg cttgcagata agtctggaaa
    38761 cattgcactt gtggagactt tagatgggaa aaaagaagta aatataatca atagtttaga
    38821 aagtaaaaga gaatattttt tacactctac taatcataca catattgata aacttcataa
    38881 attagagcca caatcaatga aaaattctat tcatagatat aaattaatta aagaatatat
    38941 aaataagtct aaaaaaattg gagagaaaga gttaatgaat ttattgtctt caaaatatcc
    39001 aaatggctta tcttgtaatt actataatga tttttttgga actttaaaaa gtatagttat
    39061 ggatttaaat ataggaaagt ttaatatttt atggggtgga ctagagaata aatgggagag
    39121 ctactattta aaaaatgaca ttaaatctat aacacagagg ataaacataa atattgaaaa
    39181 agcaccatct gacttttttg atttcattga ataaaaaatg tatttaatta ttctaaataa
    39241 ttaggatata taaatcaact aaaagttttt atttatcgta ttgaattagt tataatattt
    39301 tagtatagta aaaaatagtg ctattttaca tatttaaaga gtttaagtag aagagaggtg
    39361 ccatattgga ttacttaaag tattttgaaa aagctgtaat gtatattgag aataatttac
    39421 atgaaaatat aactgtaaat gatgtggcaa aagaaactgg atattcttat tatcatttaa
    39481 cacgcttatt taagtctatg tttggtgaga gtgtaggaag ttatataaaa aaaagacgat
    39541 tagtaagtag tacaaaagaa cttttatata gtgataaaaa agtgatagat atagctattg
    39601 gtagtggatt tgaatcttcg gaagcattta gcagagcatt taaatctata tacaaggtta
    39661 gccctataga atatagaaaa aatagaattg atgtatttgt tggtaaaaag aaaaaattag
    39721 agttagattt tatgagacat cttgttggaa atataacgat taaaccagtt ataaaagaaa
    39781 tagaaaaaat aaaggtaata ggtataaagg ataaagtaat tttagaaaat aattcattac
    39841 ctgacttgtg ggagaaattc agaaaagtac atcatattgt cccaaatact ttgccatcaa
    39901 agagagtttt tggaatctgt gaagcaatct ctgaaataca cctagttagt gaaagtatgg
    39961 aatttaatga gataatagga cttgaagtta atagttatga tgtgattcca aattcatttg
    40021 tttcaaaaac tattaaggaa ggaaagtatg ctatatttac tcatactggt agcttagata
    40081 atttagacaa aacttatgaa tatatatggg gtacttggtt tttaagttca aaagaagaac
    40141 ttgatataag agatgatttt gaagtatatg atgaacgatt tttaggtcct agtaatgtga
    40201 actcacaaat tgatatttat atacctataa aataatctat aagaaaacta aaaaataatt
    40261 tgattaagaa atagatgttc gta
//
LOCUS       DAEMIK010000018        39469 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEMIK010000018 DAEMIK010000000
VERSION     DAEMIK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.018"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..505
                     /locus_tag="KPK87_000799"
     CDS             <1..505
                     /locus_tag="KPK87_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG2536974.1"
                     /translation="VYSYGFEILIAFIVNITTMLFIGFLFGKFTYVLFFLMCYCPIRQ
                     FSGGYHADNYFRCLLTFIFIILSTILIIENINIDLFKNIIMIIASVSWVGICVLCPIE
                     HRSNPISDREKLVYKKTAIFISTVVLLITILSLSLSIFVDYFTYSAFAMFWIFVMLVL
                     GKLKAKV"
     gene            535..681
                     /locus_tag="KPK87_000800"
     CDS             535..681
                     /locus_tag="KPK87_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG2536975.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            986..2365
                     /gene="scfB"
                     /locus_tag="KPK87_000801"
     CDS             986..2365
                     /gene="scfB"
                     /locus_tag="KPK87_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG2536976.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            2394..2891
                     /locus_tag="KPK87_000802"
     CDS             2394..2891
                     /locus_tag="KPK87_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG2536977.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            3189..4853
                     /locus_tag="KPK87_000803"
     CDS             3189..4853
                     /locus_tag="KPK87_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG2536978.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            4866..7316
                     /gene="recJ"
                     /locus_tag="KPK87_000804"
     CDS             4866..7316
                     /gene="recJ"
                     /locus_tag="KPK87_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG2536979.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            7329..7841
                     /locus_tag="KPK87_000805"
     CDS             7329..7841
                     /locus_tag="KPK87_000805"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG2536980.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            7915..10122
                     /locus_tag="KPK87_000806"
     CDS             7915..10122
                     /locus_tag="KPK87_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG2536981.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            10147..10596
                     /locus_tag="KPK87_000807"
     CDS             10147..10596
                     /locus_tag="KPK87_000807"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG2536982.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            10611..11225
                     /locus_tag="KPK87_000808"
     CDS             10611..11225
                     /locus_tag="KPK87_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2536983.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            11316..12872
                     /locus_tag="KPK87_000809"
     CDS             11316..12872
                     /locus_tag="KPK87_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG2536984.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            12862..14124
                     /locus_tag="KPK87_000810"
     CDS             12862..14124
                     /locus_tag="KPK87_000810"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG2536985.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            14198..15985
                     /gene="aspS"
                     /locus_tag="KPK87_000811"
     CDS             14198..15985
                     /gene="aspS"
                     /locus_tag="KPK87_000811"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG2536986.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            16444..17676
                     /locus_tag="KPK87_000812"
     CDS             16444..17676
                     /locus_tag="KPK87_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG2536987.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            17690..18496
                     /locus_tag="KPK87_000813"
     CDS             17690..18496
                     /locus_tag="KPK87_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG2536988.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(18608..19477)
                     /locus_tag="KPK87_000814"
     CDS             complement(18608..19477)
                     /locus_tag="KPK87_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2536989.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            19849..21276
                     /locus_tag="KPK87_000815"
     CDS             19849..21276
                     /locus_tag="KPK87_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG2536990.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNDLNSQTEKELK
                     RLGVKKVYIIGLSSSISNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(21353..21622)
                     /locus_tag="KPK87_000816"
     CDS             complement(21353..21622)
                     /locus_tag="KPK87_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536991.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(21625..23067)
                     /gene="nhaC"
                     /locus_tag="KPK87_000817"
     CDS             complement(21625..23067)
                     /gene="nhaC"
                     /locus_tag="KPK87_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG2536992.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(23092..24258)
                     /locus_tag="KPK87_000818"
     CDS             complement(23092..24258)
                     /locus_tag="KPK87_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2536993.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGDYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(24483..26603)
                     /locus_tag="KPK87_000819"
     CDS             complement(24483..26603)
                     /locus_tag="KPK87_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2536994.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIAAKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            26831..27232
                     /locus_tag="KPK87_000820"
     CDS             26831..27232
                     /locus_tag="KPK87_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2536995.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            27275..27568
                     /locus_tag="KPK87_000821"
     CDS             27275..27568
                     /locus_tag="KPK87_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG2536996.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            27583..28023
                     /locus_tag="KPK87_000822"
     CDS             27583..28023
                     /locus_tag="KPK87_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG2536997.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(28115..28588)
                     /locus_tag="KPK87_000823"
     CDS             complement(28115..28588)
                     /locus_tag="KPK87_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2536998.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYKKSK"
     gene            complement(28588..29376)
                     /locus_tag="KPK87_000824"
     CDS             complement(28588..29376)
                     /locus_tag="KPK87_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2536999.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            29704..30948
                     /locus_tag="KPK87_000825"
     CDS             29704..30948
                     /locus_tag="KPK87_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG2537000.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            31271..32368
                     /locus_tag="KPK87_000826"
     CDS             31271..32368
                     /locus_tag="KPK87_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG2537001.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            32359..33057
                     /locus_tag="KPK87_000827"
     CDS             32359..33057
                     /locus_tag="KPK87_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2537002.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKKVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            33060..34124
                     /locus_tag="KPK87_000828"
     CDS             33060..34124
                     /locus_tag="KPK87_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2537003.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            34307..35011
                     /locus_tag="KPK87_000829"
     CDS             34307..35011
                     /locus_tag="KPK87_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537004.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            35004..36326
                     /locus_tag="KPK87_000830"
     CDS             35004..36326
                     /locus_tag="KPK87_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2537005.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            36513..37886
                     /locus_tag="KPK87_000831"
     CDS             36513..37886
                     /locus_tag="KPK87_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBG2537006.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(38258..39196)
                     /gene="pdaA"
                     /locus_tag="KPK87_000832"
     CDS             complement(38258..39196)
                     /gene="pdaA"
                     /locus_tag="KPK87_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG2537007.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        15071 a         4546 c         6721 g        13131 t
ORIGIN      
        1 agtctattct tatggatttg aaatattaat agctttcata gttaatataa ccaccatgct
       61 gtttataggt tttttatttg gtaaatttac atatgtatta ttttttttaa tgtgttattg
      121 tccaataaga caattttcag gagggtatca tgcagacaat tattttagat gcttattaac
      181 ctttattttt ataattttat ctacaatctt aattatagaa aatataaata tagacttatt
      241 taaaaatatt attatgatta ttgcaagtgt tagttgggtt ggaatttgtg ttctgtgccc
      301 aatagaacat aggagtaacc ctataagtga tagggaaaaa ttggtttata agaaaactgc
      361 tatatttata tctacagtag tgttattgat aacaatttta agtctttctt tgagtatatt
      421 tgtagattat ttcacatata gcgcatttgc catgttttgg atttttgtga tgttagtatt
      481 aggtaagtta aaagctaaag tataagtaaa atatgtatga gggagagtta agttatgaaa
      541 aagtttattg taagatttat gaagtttgct agctcattgg cacttagcac tgctattttg
      601 tcagctaata gtacctgtcc ttggattatt catcagccta aagtacccaa agaaatcagc
      661 aatctaaaga agacaaatta aatacaagca cttatttata caaaattatc aggaaaaaag
      721 aaattattat attttaataa gattaaaaca atataaaatc agatacaaaa tttattttat
      781 ttattttata ggagcctaat acctatttgg taatatatta gtgcgccaat ttagggggat
      841 atagtgtatt taatgaaatg acttacggag agtaagtcat ttttgtattg tatgtatttt
      901 atgtaaactt atgatataat gaagggtaaa gtttaattaa aataataaaa aactttagta
      961 tagatagtaa tttaggaggt agggtatgag tatgatacat aaattttcta tgaatggata
     1021 taatatcgtt ttagatgtga atggtggagc tgttcatgtg ttagatgatg ttgcatacga
     1081 tttgttagat ttttataaag aaaaaagtaa agaagaaata ttagagatat taaaatcaaa
     1141 atatcaagaa gaaaaaataa atgaagcata tgaagaaata ttaaatttgg aaaaagaggg
     1201 tcttttatat acagaagata catatcaata tcatccaagc tttgtacata gagagcctgt
     1261 agtaaaagct ttatgtttaa atgtagctca tgattgtaac ttaaaatgta aatactgttt
     1321 tgcagctcaa ggtgattttg gaggagaaaa agaacttatg agttttgaag ttggtaaggc
     1381 agcaattgac tatttgattg ctaattcagg aagtagaaag aacctagaga tagatttctt
     1441 tggtggagag cctttgatga actttgaagt tgtaaaacaa ctggttgact atggaagaag
     1501 tgtagaaaaa gattataata aaaatataag atttacaata actacaaatg gtgtactgct
     1561 aaatgatgag attatagatt atataaatga aaatatgcat aatgtagttt taagtttaga
     1621 tggaagaaaa gaagttaatg ataatatgag accaacttta aatgataaag gaagttatga
     1681 tattacatta ccacgattta agaaacttgt ggaaaaaaga gcaaaagata agtattatta
     1741 tataagaggt acttttacaa gagataattt agatttttca aaagatgtta tgcattttgc
     1801 agatttagga tttaaattaa cctctgtaga gccagtcgtt ggagatgaga gtaatccata
     1861 tgcacttaga gaagaagatt tacctaagat atttgaagag tacgaaaaat ttgctgtaga
     1921 gtatgcagat agacaattac aaggagatgg atttaaattt ttccatttta tgatagacct
     1981 aaatcaaggt ccatgtgtaa taaagagaat aactggatgt ggagctggaa atgagtatct
     2041 ttctgtaact ccaaatggag atatctatcc atgtcatcaa tttgttggaa atgaagaatt
     2101 taaaatggca aatatatttg atgaagaaat agtacttcca gaaaatttaa aaaatatgtt
     2161 tagagaagct catgtatata ctaaagaaga gtgtaaacaa tgttggaata agttttactg
     2221 ttcaggtgga tgtcatgcaa atgcgataaa ctttaataat gacatatcaa aaccatatga
     2281 attaggctgt gaaatgcaaa gaaaacgtac agagtgctct ataatgatac aagctaagtt
     2341 aatgttagaa ggagcaacca actaaattta tattttaaag gagttgttta tatatgatta
     2401 gagattattt agaagataaa cctttaattg acgaaagtgt atttgtggca aaatcagcag
     2461 atgtaattgg aaatgttaaa ataggcaaag attcaagtat atggtacaat gcagtagtta
     2521 ggggtgatga aggaccaata actattggag aaaacactaa tatacaagat tgttctatag
     2581 tgcatggaga tacagaaact ataattggta acaatgtaac agttgggcat aggtctatag
     2641 ttcatggttg taaaattagt gataatgtgt taattggtat gggttcaata atattggata
     2701 atgctgaaat aggagaatat actctgatag gagcaggtac actaattaca tctaataaga
     2761 agtttccacc aggtgttttg ataatgggtt ctcctggtaa ggttgttaga gagcttacag
     2821 aagaagataa aaaatatatt gatgaatctt atgaatggta tttagaggca gcacaaaatc
     2881 aaaaatatta gtttaaagag tggttgtttc aaaacatatt taaatctgtt ttgaggcaac
     2941 cactaattta ttttattaga ttttgttgat attttagttt tcaaaaagta tttgattaaa
     3001 ccagatataa aatgtcaaaa ataaaaatta actgagttaa ataattttaa taaaattggt
     3061 agtatatgtg ataaaattaa atattaaaga ggatatagga tattagaata tcaattggtg
     3121 aaataatgtc atatacgata aattaaggtg taagtttttg ggtataccta tcttaggagt
     3181 gaaaagattt gagaataagt tatagaaatt taaagcgata tagagaaatt ggatatgttt
     3241 taataaagta tggttttagt tttattgtag aaagactaaa tatagaagga attgcctata
     3301 aaataccact atttgaccca ccagaagaaa taaaaaatat gacaactggt gagagaatga
     3361 aaagagtttt agaagaacta ggacctacat atataaaaat aggacagata ttgagtacaa
     3421 gaaaagactt gttagaccag gatataattg atgaaatatc aaaactcaga gatgatgtag
     3481 agaaatttga ttcaaatata gctattgata tatttaaaga agaagtgggt ttaagtatag
     3541 aagaaatatt cttagaattt aaagaagaac caatagcagc agcttcaatt ggtcaagttt
     3601 atgaaggtgt acttaaaacg ggagaagaag taatcgtaaa aattcaaaga ccaaatatag
     3661 agaaaataat aaaatctgat ttagagattc ttagaactat tgcaaatact ttaaaagatt
     3721 taaaaaagga ttttaattta gatttagtac agatgattga agaatttcaa actcaattaa
     3781 tgagggaact tgattataca tttgaagcta taaatgcaac taaattctct aggatattta
     3841 aaaatagtga tgaagtatat atacctaaag tatactcaga atataatact aagaaaattt
     3901 tagtaatgga gaaagttaat ggtacaaagt taagtgatgt agaaaaaata aggagactag
     3961 gttataacac aaaaactatt gttgaaatag gcgttaggtc attttttaca caggtattat
     4021 ctcatggatt ttttcatgca gacccacatc caggtaatat attcgtagtt gctaaaaata
     4081 agatagcata tatagatttt ggaatgattg gaattattga taataaaact ttaaaccaat
     4141 taaatgaaat tgcattagca ggtgtagaaa agaatgttga caagataata tatctattga
     4201 tagaaatgga tgcattaaat ggagaggctg atattaaggg acttagacag gatttacttt
     4261 atctaataca ttattactat gatatttcta tagaaaaaat aaatgtaaca gatatattaa
     4321 atgaactatt taggtttttt agacagtata aaatagtcat gcctgcacaa tttgtaactc
     4381 ttgcaaagac agttataaca ttagaaggaa caagtagaac tttaaacaca gatttttctt
     4441 ttggttctat gggaaaagag tttatgaaac atcattataa gagtaaattt aaccctaaaa
     4501 atgttgtttt aagttcaaga caaaatgtag aagaaatatt actggacata aaaactattc
     4561 caaagcaatt aaaagcaatt ttaaaaaata tagaaagaaa taatataaaa atgcaaattg
     4621 aagatgttaa aatgacgagg ttagaaaatt gtataataga gctaacatct caaatatctt
     4681 tgagtttagt gttggcatct attatagtag gttcttctct tataattgca tctccaaata
     4741 tagaaaataa tatatggata aaatttactg caatagcggg tttctttata tcctttataa
     4801 taggcctttg cttagttata agaagtatta ggtcaaaata caaaaaagat tagggggaat
     4861 taaatttgaa aaaatggata ttgaagtata aaggtaagat agaggaaagt gaatttagca
     4921 aaaaacttaa tatttccccg gaaatctgcc aaattttaaa aaatagaggg atatgtactg
     4981 aaaaagattc agaaatattt atgaatccat ctttagatta tttaagagac ccttttttaa
     5041 tgaaagatat gcaaaaagct gttgatagaa taaagttagc agttgagaag aacgaaaaaa
     5101 tttggattta cggagattat gatgttgatg gtgtatcatc tacatctatc ttgtgcttat
     5161 attttaagag tataggttac aatgttgatt actatattcc taatagatta gaagaagggt
     5221 atggtataaa tgaagatgct attaagctta taagctctag gggatgtgac ttgattataa
     5281 gtgttgactg tggaattaca tcagtgtctg aggtcaatac agcgaatgat ttgggcatag
     5341 atgttataat tacagaccat catgaatgtc aaagtgaaat tccatcagcc tttgcagtaa
     5401 taaatccaaa acaagaagat tgtaattatc catttgattc actatgtgga tgtggtgtag
     5461 catttaaaat gattcaagcc ttgactccaa aagaagaatt taaaactagt atgtataatt
     5521 atttggaaat agtaacacta gcaactattt gtgatattgt tccacttata gatgaaaata
     5581 gaataattgt aaaaaatggt ttaaaatcaa tgaaagaagg caaaaatatt ggattaaggg
     5641 agcttataaa agtttgtggt gtagaaagtg ataagatagg ttcttctcat ataggttttg
     5701 caataggtcc taggataaat gcttcaggtc gattgggata ttcttactta ggagtagagc
     5761 tatttactac tcaatcacaa gaagaggcag tagaaatagc tagtatattg gaagaaaaaa
     5821 ataatgaaag acagatgata gaagctaaga tgtatcatga agctgaagaa atgctaaagt
     5881 ctaatagcag atacaatgat gacaaagtgt tggttttagc taaagagggt tggcaacatg
     5941 gaataatagg aattgtagca tcaaagttaa ctgaaaaata ctacaaacca acaattcttc
     6001 taggtataga aaatggggag gctactggtt cagcgaggtc aataaaagga tttaatatat
     6061 ttgaagctct cataaagtgc aaagacttga tgactaagtt tggtggtcat gaacaagcag
     6121 cagggttatc cttggattca gacaatgtag aaattttagc aaatgaaata aataaatttg
     6181 cagattataa tttaactgaa gatgatatga tagagaatgt aaatgttgag tttgaattac
     6241 aagaaaatgt aataaattta aatcttgtag aagaattaca taagttagaa ccctttggat
     6301 taaataatcc taatcctaga tttatagtta gaaattatat tttaaaagat ttgaaagtta
     6361 taggtaagaa tcaacaacat ctgaaattaa gtatagaaaa agagaaaagc tatgaatgta
     6421 taggatttaa tatgagtcat ttaaaatcaa tgtataaagt aggagacaaa gtagatgtac
     6481 tattccaatt agatgaaaat aactatatgg gaaataggaa agtacaattt ttacttaaag
     6541 atatacgatt ggctcgacct aaaagtgcaa gtaatgacaa actttcattg aaattaatgt
     6601 caaaaataat accaaaagat actcaaagcc tgtataatat atctgtgtct gattttgaat
     6661 tatttgatgg aaatacagat ataaatatat ttgattattt tgaaaaagat acactaataa
     6721 tatctaattc tataaatgga ttttatagag caatgtcaga tatatcactt atagatttgg
     6781 attttaacat aaattacaat attattgaag atgttagcaa aaatactgat aaattagagc
     6841 ttatattttc tcctaatatt gataaaatag acttaaaaag atataataat attattcttt
     6901 atgattattt atacaataaa ggcgaatatt cgtatatata tgagaataaa agagaagaaa
     6961 gtgaaattat aaaatattat aataaaacag acctattata tttaaagaat gtagttagta
     7021 atatagttcc atcaagagat gaatttataa caatttataa acaagcactc ataaaaaaag
     7081 aaattgattt ggatatggta aatataaggg aaacttttaa tgtaattcca ttaaagtttt
     7141 ttacgatatt aaatgtattt agagaattaa atttacttga ctttaattta aattatgaaa
     7201 aaaattctgt actcataaga atattgccaa aaccacagaa aaaattagac ttaaatgaga
     7261 gtttaatact caataatcta aaaaatttag aaaaacaata caatagcagt tattaggagg
     7321 cgtttcccat ggatttaaaa aactttataa ggaatattga tgatttccca aagccaggca
     7381 tagattttaa agatgtgaca actctattta aagatggaga tgcatttaaa tatgcagtgg
     7441 attctatagt tgaagaattg aaagataaag atgtagattt agtaatagga ccagaagcaa
     7501 gaggattttt gatgggtaca cctgtagcat atgctttagg agtaggattt gttccaatta
     7561 gaaaacctgg aaagttacca ggtgaagttg agagttatga gtatggatta gagtatggta
     7621 cagatacttt agagatacat aaggatgcaa ttaaaaaggg tcaaaaagta gcaatagtag
     7681 atgatttatt ggctactggt ggaactatgg aagccgctgc caagcttgta gaaaaattag
     7741 gtggagaagt agtttctatg caatttttga ttgagctaaa attcttaaat gggagagaaa
     7801 agttgtctaa ctatgatgtt aattctctta taaaatacta gtacttttat aatagttggt
     7861 ataacaccaa ctattttcat atatattaaa attttataca aaataggtgg ttatatgcat
     7921 gataaagaat tacaagaaat aattgaaaaa ataaaagttt atgcacctaa tgcaaatttt
     7981 gaaatggttg aaaaagccta taatcttgca aaaaaagcgc atgaaggaca atatagaaaa
     8041 tctggggaac catactttat tcatccatta gcagttgcaa atattttaat agagatggaa
     8101 ttagatattg aaactataac agcaggttta cttcatgatg ttgtagaaga tacagaatat
     8161 acctatgaag acataaaaaa agaatttggc aaagaagtag cggatttggt tgatggagta
     8221 actaaacttg gtcaaataaa atatcgttcg aaagaagaaa ctcaatctga aaatttaaga
     8281 aaaatgtttt tggcaatggc gaaggatata agggttatac ttataaaact tgctgataga
     8341 ttgcacaaca tgagaacatt aaaatacatg cctccagaaa aagcaaagta taaagcaaaa
     8401 gagacacttg aaatttatgg tggtatagct catagattag gaatttccaa gataaagtgg
     8461 gaactagaag acagagcatt aagatttatg gacccagaag ggtattatga tttagttagt
     8521 agagtatcaa tgaagagaag tcaaagagag gattatattc aaggaattgt tgaacttctt
     8581 aaagataagt ttaaagattt aagtatacca tgtgatgtat atggtagacc aaagcatttt
     8641 tacagtatat atagaaagat gcaaaaaaaa cataagactt ttgaagagat ttttgattta
     8701 acagcagtaa ggatattggt tgataatgta aaggattgct atgcagtact tggtatagtt
     8761 catacattat ggagacctat tccaggaaga tttaaagatt atatagcaat gccaaaaccc
     8821 aatatgtatc aatcattaca caccactgtt gtaggtcctg atggagaacc tttagagata
     8881 caaataagaa ctcatgagat gcataatata gctgaaaatg gtatagcagc ccattggaaa
     8941 tataaagaag gtatctctag caaagagtca aaggttgaag aaaaacttca atggttgaga
     9001 cagatgatgg agtgggaaaa agatttaaaa gacccacaag agtttatgga tgcacttaaa
     9061 gaagatgtat ttaatagtca agtttatgta tttacgccaa aaggagacgt aattgaatta
     9121 ccagcagaat caacacctat agattttgcc tatagggttc atacaaatgt aggtaataaa
     9181 tgtgtaggag ctaaaattga tggaagaata gttcctattg attataagct tcaaaatggt
     9241 aatattgtcg aggtacttac atcatctaat tctaatggac caagtaggga ttggcttaat
     9301 atagtgaaga ccccaaatgc aaaaagtaga attagacaat ttttcaagaa ggaaagaaga
     9361 gaagaaaatg ttgaccgtgg aaatacaata ttagaaagag aatttaagaa gcatgatata
     9421 ccacaaaaag accctcttgt tgaaaaatat atggcattag ttgctaagaa atttaatcag
     9481 ccaagtgttg aggacttaat tgccactgta ggttatggtg gtataatgtc atctcaagtg
     9541 gtatctaagg taaaagacct atatttaaaa gaggttaaaa aagctaaaaa ggaacaacag
     9601 atacaagaag aagatttaaa taaacacaat ataagtgaaa cagaatacag taaaaagaga
     9661 aagaaaaacg catcacaagg aattatagta aaagggcttg acaatatctt agtaagattt
     9721 gctaagtgtt gtaatcctct tccaggagat gaaattgtag gatatattac taagggtagg
     9781 ggagtagcca tacacagagc tgattgccca aatgcaaaca ataatggtga tttcttccaa
     9841 aatagattgg tggaggtttc atggagttca gcaggaaaag gtaagtttga agcggaaatt
     9901 caaataaaag ctattgacag aagaggaatt gttaatgaaa taactcatat tgttgcaatg
     9961 gataaactta gtttaaacgg aataaatgca agaaaaggta aggatagtat tgtaagtata
    10021 aacttacttg tagaggttaa tgacacagag gaattaaaaa ttctaatgaa aaaattaaaa
    10081 gcaattcctg gtgtagaaag tatttataga atgacaaatt agagttaaaa taatagggag
    10141 gaaactatga gagcagtagt gcaaagagta tcttcatcta aagttacagt agatgaaaac
    10201 actataggtc aaataaataa ggggttacta gtattgttag gagtgactca tgatgataaa
    10261 tcaagtgatg tagattatat gattgataaa atattgaatc tgagaatttt tgaagatgaa
    10321 aatgacaaga tgaatttatc tctaatggat ataggtggag agcttttagt agtatctcaa
    10381 tttactttat atggagattg cagaaaagga agaagacctg gatttagtaa tgcagctaga
    10441 ccagaacttg caaataacct ttatgaagaa tttgtaaaaa aagcaaaaga taaaggtgtg
    10501 acagttggta caggtcaatt tgcagctcat atgatggtag agttaactaa tgatggtcca
    10561 gtaacaatac tattagactc aagtaaatct ttttaagggg gaaaacttta atgattataa
    10621 aaaaatttgt agatagttat tttggagtaa atacttatgt attaggtgat gaaaagacta
    10681 aaaaatgtgc agttatagac ccaggtggaa gtttagtaga gacattaagt tttgtaaaag
    10741 aaaatgaatt aaatattgaa tatattatcc ttactcatgg gcatggagac catataggat
    10801 atgttaagga tattaaagaa aaaactggtg ctaaagtagt tgcacatgtt gatgaaaaag
    10861 aattattaaa tgacaaaaat aaaaatctaa gttatacaat gagatgtgga gcacaagagt
    10921 ttgatgcaga tatctatgta aatgataaag acaagcttga acttggtgaa ttaaaaatga
    10981 ctttcttgca tactccagga catactcaag gatgtatgtg tataagagtt gaagatgaaa
    11041 tgttcacagg agatactttg tttgctggaa gtattggtag aactgattta tatagtgggg
    11101 actttaacca aatggaaaaa tcacttaaga aattatgcaa atatgaagat gctgttagag
    11161 tatatcctgg acatggacca accagtactc taggggttga aaagaagaca aatccttaca
    11221 tgtaatttta tattagtatt taaaaaagta taaagtattt aataactata ttagtgaggg
    11281 ggttcttcta ctgttaaata gaaggagtta agttgatgtt gtatgttttt ttaaaaggac
    11341 atgattataa gtatgaagta gcagaactta taaaattgtt taccagtgaa tttaagttta
    11401 aagactctaa tgctaacaat aatatagagt caaagcattt aataaataga ttgatttatg
    11461 agaatggtgt attattctca agtactgaat actatgaaaa tggaaactta aagtacgagt
    11521 caatacagaa tatagaaaat atgaatcttg aatttttaga tggatttgat agcagcaaaa
    11581 cgattgaaga atttcttaat agcctagata gtgaaagtaa gaggaacttt aaaaagctgt
    11641 gtaaagaaaa tatcaaaaaa agcatgtttg tagtgcttaa acaagtattt aattcttatg
    11701 taccatgggg aattttgact ggtattagac cagtcaaaat tgtccacaat ttaatggata
    11761 aaaagttaga tgataatagc ataagaacca ttctaaaaga taattatttt ataatagatg
    11821 agaaaataga tttagctctt gaaatagcta aaagagagcg attatttatg tatcctatag
    11881 ataaaaataa aatttctctt tatgtaagta ttccattttg tccaactaga tgtgtatatt
    11941 gttcatttcc ttcaaattca ttaaaacaat ttggacattt aaaaagagaa tatgtttata
    12001 aattgattga agaaattaag gggtttgcca aagttattaa ggagactaag aaagaagttg
    12061 aaactctata tattggtggt ggaactccaa ctacacttga tgaggaagaa ttggatttgc
    12121 ttataaattc attgttcaat gaattagatt taagtaaaat taaagaattt actgtagaag
    12181 caggtagacc tgacactatt acagaacaaa aactaagagt gttaaaaaaa cacaatgtta
    12241 gtagaattag tataaatcct caaactatga atgatgatac cttggtaaag attggaagag
    12301 agcatagagt atctgattta gtagattgct ttaatatggc tcgtagaatg ggatttgata
    12361 atataaatat ggatataatt ttaggtctta tagatgaaga tttaaatatg gtaaagaata
    12421 cattggagga gataaaaaaa ctttctcccg aaagccttac agttcataca ttagccataa
    12481 agagagcatc caacttaaat ataaatatgg ataggtataa agaacatcta acacaatatg
    12541 aagaaatggt aaaaatgata gacttatcaa tgaaatatgc aaaagatatg ggattaaatc
    12601 catattatat gtacagacaa aaacatatgt tgggaaattt agaaaatata ggttatgcta
    12661 aagaaggata tgagtgcatt tataatatac agattatgga agaaaaacaa agtaactatg
    12721 cattaggtgc aggtgctata tcgaaatttg tttatgtaga tgaagataga attgaaagag
    12781 tagaaaatgt aaaaaatgtt gaacaataca ttgaaagagt agatgaaatg ataaaaagga
    12841 agaaagaaga ggtatataaa aatgttgact aaagctccta gaggaactaa agacataact
    12901 ccaaaagagg cttacaaatg gcgttatgta gaaaataaat ttagagaaat atgtgcttta
    12961 tatggatatg aagaaatggt aacacctata tttgaacata cagaactttt caagagaagt
    13021 gttggagata ctacagatat agttcaaaaa gaaatgtatt cttttaaaga taaaggtgat
    13081 agagaaatta ccctaaagcc agaaggtaca gcaggtgtag taagagcatt tatagaaaat
    13141 aaattatatg cagatactca accaacaaaa ttattttatg tgacaccttg ttttagatat
    13201 gaaagaccac aagctggtag acaaagacaa tttcaccaat ttggaataga agcacttgga
    13261 agtgatactc cttcaatgga tgctgaaata atcgcactag ctgttcaatt ttttaatgaa
    13321 gttggattaa atgatttggt agtgagtata aattcagttg gctgtccagt ttgtagaaaa
    13381 gagtacaatg ctttattaaa agagtatctt gactcaaaag ctgatatatt gtgtgatact
    13441 tgcaatgaga gaagagaaaa aaatccaatg agggttatag actgtaaaaa tcctacatgt
    13501 aaagaaaata tcaaagatat accatttatt gcagaccatt tgtgtgatga ttgtaaatct
    13561 cactttgata aacttcaaga atacttaaag gaaatgaata taaactttgt aattgataaa
    13621 acaatagtta gaggcttaga ttactataga aaaactgctt ttgaaattat atctaatgat
    13681 ataggagctc aaagtactgt atgtggtgga ggaagatatg atggtcttgt tgaacaatta
    13741 ggaggaccaa aaggtataag tggaattggg tttggactag gaattgaaag acttttactt
    13801 acacttgaag gtaatggaat tgaaatagaa aaccctcaat ctacggatat atttatagta
    13861 actataggtg aagaagcaaa tacaagaagc tttaaattgt taaaagactt gagacaaaat
    13921 catataagtg cagataaaga ccatatagag agaagtgtta aagcacaatt taaatattca
    13981 gataaaataa attcaaaatt tacaatagta attggagatg atgaacttaa aaatgacact
    14041 gcgacattaa aaaatatgaa aacatcagag caaactactg taaaattaag tacattagtt
    14101 gaagaattaa agcaaaagct gtaaaataag aaagtatgat gttttgtgta ttagataaat
    14161 atatagcatt aaaattaaat aaaatggagg tagtatcttg gaaactttaa agggtttaaa
    14221 gagaactcac tactgtggag agttgagaga aaaaaacata aatgaagaag tcgtacttat
    14281 gggatgggta cagaaaaaaa gaaatttagg tggtcttgta tttgttgatt taagagatac
    14341 aagtggatta tgtcaaatag tttttgatac agatgtagat aaagaagctt ttgaaaaagc
    14401 tgaaaaatta ggggctgaat ttgtaatagc tgtaaaagga aaagtatgcg aaagacaatc
    14461 taaaaacccc aatatgccaa ctggtgatat agaaatattt gcaactgaac ttagactttt
    14521 aaacaagtca gaaactccgc ctatttatat aaaagatgat gatgatgttt ctgaagcact
    14581 aagattaaaa tatagatact tagatttaag aaaaccatct atgcaaagaa atttaaaatt
    14641 aagacataaa gtaatgaata ttacaagaaa ttacttatca aacaatagat tttgcgaaat
    14701 agaaacacca tttttaatag ctccaacacc agaaggagca agagattatc tagtaccaag
    14761 tagagttaat ccaggaaaat tttacgcatt accacaatca ccacaattat ataaacaatt
    14821 attgatggta agtggtatgg atagatattt ccaaatagtt aaatgcttta gagatgaaga
    14881 cttaagagca gatagacaac cagagtttac acaaatagac tgcgaaatgt cttttgttga
    14941 gcaagaagat gttatgagta tgattgaagg tttgcttgaa gcgatattta aagaagtttt
    15001 agatgtagaa ttggcacttc cacttcctaa gatgacttat gcagaagcta tgtctaaata
    15061 tggttcagat aaaccagata ctagatttgg atatgagtta acagatatat ctgatgttgt
    15121 ttgtaactgt ggatttaaag tgtttgctga tgctacacaa ccaggaaaaa gtgttagagg
    15181 tataaacgta aaaggcaaag ctgacgactt tactagaaaa caaatatcat cactagaaga
    15241 acatgctaag acatatagag caaaaggtct tgcttggatg aaagtaggac aagaaggagt
    15301 aacttctcca atagcaaaat tcttcaatga agaagaaatg aacgctatac ttacaagaat
    15361 gaatgctgaa gtaggagatt tacttttatt tgtagctgat aaaaactcaa tagtatttga
    15421 tgctttagga caagttagac ttgaagtagc aaataggctt aatctattgg ataaaaatgt
    15481 atataactta ctatgggtaa ctgagttccc agtttttgaa gaagatgaag aaacaggcac
    15541 attctcagca atgcatcacc catttacatc acctatggat gaagatttag acaagttaga
    15601 agaaggtgat aagtcatctt taagagcgaa agcttatgat attgtattga atggttatga
    15661 aataggtgga ggaagtgtta gaatatcaaa ttctgatgtt caatcaagaa tgtttaaagc
    15721 acttggtttt acagaggaaa gagctaatga aaagtttggt tatttattag aagcatttaa
    15781 atatggtact cctcctcatg ctggtctagc ttttggtctt gatagacttg ttatgttact
    15841 tgcaggaact gataatataa gagaagttat tgcattccct aaaaatcaaa atgctgtttg
    15901 ccctatgaca aatgctccta cattggcaga agatgagcaa ttagaagaat taagtataaa
    15961 agttgatata aaagataatg aataatatgt agttgcaaga aactaaaatg taaaaagtct
    16021 ggatataaag tatatttata ttcagacttt ttttattaga attattttat attttttctc
    16081 tatatatttt cataaattat acttgtattc tcaaccaata taattaaaat actatgttta
    16141 aaaattttac ataaacttgg ttgttttaga aactattttt ttaatgagta cacgatttta
    16201 attaaagaat atataacaaa tttgaaataa gtctaatata tgacttaata gattaaatta
    16261 aaataataaa tttggacgat ttatctattt ataacttaaa ttatatcaaa ttgataacaa
    16321 actagatatt atactttttt gtagaataga attacaatta tataaagaaa aattctatta
    16381 atagtgaaaa aaaagtagaa aaaagtagaa attattcttt tataaagaaa gggagggata
    16441 atattgcaag agagagaaga attagtttta aatccagttc caaaagagga aagagttgga
    16501 tggttagctc cattgtttaa tatgcttggt tctaacatag ctatatctga actaatggtt
    16561 gggggaactt taattttagg aatgactctt tctaatatga ttattacatc aattataggg
    16621 aacttaatat tagttgcaat tattatgata caaggttata taggttacaa agaagggtta
    16681 aatacgtatg tattagctaa gggggcattt ggagagatag gaggaaagta tcttatatct
    16741 ctattacttg gtataactag ttttggatgg tttggtgtac aagcaggggt tgcaggacta
    16801 tcagtacaaa agatattccc aagtgtaaac ctaacattag ttacagttat acttggatta
    16861 tttatggttg tttttgcact gtatggtttt aaagctatgg ctaaatttaa ttacttagtt
    16921 ataccaccac ttatgatatt aatggtgtgg ggagttttta aagctttttc tacttatgga
    16981 gttgaagcta tttataatta tactcctcaa acaactatga gtatggttga aggtcttaat
    17041 attgtagtag gtcttgttat agttggtgca attatatcac ctgaccagtt aagatatacg
    17101 agacgagtaa aagatatttg gattatcagt tttttaggtc tagggattat ttcattattc
    17161 caacaagttg cagctggtgt aatgtctatg ggagcaccta cttgggatat aacagaagta
    17221 ttggctaatt taggttttgg atgggtagcg tttgttattt taatacttgc atcttggtct
    17281 acaaacgtat caaatgctta ttctggtgga ttagcattaa aaactatttt tccaaatgta
    17341 aaaagaaatg ttttgacttt agttgcaggt ctaataggta caataatagc agctactggt
    17401 ataatattta aatttcagtc atttttatct ttcttgggaa tcgcagttcc tgcaatagct
    17461 ggaattatgt ggtgtgagta ttattttata caaggaagaa cttataagca tagagaagga
    17521 ataaattgga ttgcagtaat aagctggtta ataggttttg cagcgtcgta ttatagttca
    17581 aaaataaatt tcttaatacc tccgataaat ggaattgtag tgagtatggt tatttattat
    17641 atattaatga aatgttttgg aatcaaagat aaataaaggg ggcaattaca tgggtcgtaa
    17701 ggtgaaaata ggtataattc aacaacacag tgtacttgga aatgttaaaa aaaatataga
    17761 aaaggcagta gagatgatag atgacttagg aaaacaaggg gcagatataa tttgtttacc
    17821 agaactattt gctacaggat ataatttaga gtctttaggt ggagttaaaa cattagaatt
    17881 aataagagaa cataataagt acatagagga atcaatgtca gaagctgcta aaagaaataa
    17941 tgtatacctg atatctccat atggaacctt agaaaaaggg tctacacatg tttataattc
    18001 agcagttata tttgatagaa aaggtaagat tatgggagaa tactgtaaaa atcatctgtg
    18061 gtcattagaa gcagtatact ttaaaggagg ggaaaaagta gaggtatatg atgctgattt
    18121 tggtagattt ggtgtaatga tatgttatga tgcaggtttt ccagaagtat ccagagaatt
    18181 gacattaaaa ggttctgaaa taatatttat accatctgca tggagaatac aagatgaaga
    18241 tatgtgggat ttaaatgtat ctcaaagagc tttagagaac actgtatata cagtaggtgt
    18301 aaatttagta agtaatgact ctaatttaat actatttgga aagagtaaaa tatgcaatcc
    18361 tagaggtact gtaataactc aattagatac atacagggaa aaatatgtat tatcagagat
    18421 tgatttagat gaaataaaaa agtataggga agatatcccc tatttgaaag acagaaaaag
    18481 tagctataac atataattct atactataat acaagttcaa tataaaatga taaaaagagg
    18541 atgtatcaat attaagatta aatttattat actttgatat atcctcttta atattatcaa
    18601 ctttgattta aatattttta aaatagttga taagaaagtt tttgaacaac aacacagctt
    18661 tagattcata gttttcgcta aaccatgaaa gatttaaata tcttttaaat tcaatatctt
    18721 tgatatgtaa caacttaatc tcagggtctt gagataatcc ccatgatttt ccacatataa
    18781 atccaactcc ttgaagtgat tttataagag cataaattgt atatggtgaa tcagactcaa
    18841 atgctatttt gggtttgaaa ttagctgatt cgcacaggat atcaattacc tctctataat
    18901 tctcaccctt cgttataact ataaagtttt catctgaaac ttcgcttaga tatacttcat
    18961 cctttaatga taaaggatgg tttattgaaa ctcctaatag tatttcttcg catgttaaag
    19021 ttatgctatt ttctgagttt agctttgtaa atgaagaaga tatgtacaaa tcaaaatcac
    19081 tttttttata agatgatggc aaagtatgtg acacattaaa agtaatattg ggatatagtt
    19141 ttctgaaatt actcaaaagt gatggtaaca cattagccgc tgcaggagat aatatcttta
    19201 cttctcctga attttctaaa gaagtgtctt gtaactcctt ttttgaactt tctactaaat
    19261 ctaaaatgct atctactctt tttaaaaaca atcttccata ctcatttaaa acaatatatc
    19321 ttccttttct gtcaaataac tgaactccaa gttctttttc taaggaagat atagttttgc
    19381 ttaaagctgg ctgtgctata tttaacttgt gtgatgcttt tgttatgtgc tccattctag
    19441 caacagtttg aaaatactta agatgcaata aatccatagt ataaaataca cctcttttaa
    19501 tatgacttga gttatatcaa ttttataatc aactccacat tatacattat aatataaagt
    19561 ttatataata aactacaata tattaaaaag ttatttaaat tgttatttat attttgtaca
    19621 taatctaatt aaaaaaagta actttaaatg attaactaag gaaatgttgt tacaaacttt
    19681 agttatataa aaaattcatt tgatgagcaa agtattaatg tcaagtaaga ataaaagctt
    19741 attagataag aaaaataata ttttataata atgatgttac ttaaattatt atgttatact
    19801 attttaaata ggaaacattt agatactata attttagggg gagtcaagat gaaattaact
    19861 aaatcaatat catcaatgct gtgcttatct ctagttttaa gcttatcacc agtagcatat
    19921 gcaaatgaaa taagtagaac aacagttgag aatactttag taggtcagga taggtatcaa
    19981 actgctgttg aagtaagtaa aaaaggatgg agtagtgcaa atgaggctgt agttgtaaat
    20041 agtgaatcta ttgtagatgc attagctgta acaccatttg ctaaattaaa aaatgctcct
    20101 atattattga cagaaaaaaa tgatctaaat tcacaaactg agaaagaatt aaaaagatta
    20161 ggagttaaaa aagtttatat aattggtcta tctagttcta tatcaaatga tgtgcaaaaa
    20221 cagttagaat ctaataattt aactactgat agagttggtg gtagtgatag ataccaaaca
    20281 gctttgagta tagctaaaaa aatagaagga ttaaaagata tatcagaaat agctgtagta
    20341 aatgggtaca caggtcttgc agatgcagtt agtatagctt ctgtagctgc tactaatgga
    20401 atggttatac ttccagtatc tgatgcatct ggtatatcta gctttaaaga ttttatagaa
    20461 tctaagaata taaataaatc atacataatc ggctctacta atgctgtatc agataaaata
    20521 aaacaaagtt tacctaattc agaaagaata ggtggagcag atagaaatga aactaatggt
    20581 aaagtaatag aaaagtttta tacatctaat aaattaaaca atgtatttgt ggctaaggat
    20641 ggaatgaaaa atcaaaatca acttatagac gctctagcag taggagtact agcatcaaaa
    20701 gaaaattctc ctgtactaat cgtaggagaa caattaagtg caacacaaaa gagtgtattt
    20761 tcgaataaaa atgctacctc tataactcaa gttggtagtg gaggaaatga aaatgcattt
    20821 aaagaattaa aagatttaca ggcaaatagt aacaataaga gagtaatgta tgtaactaat
    20881 acagataagg taaatataag aaatgatgct acaatagaag cttctgttat aggatatcta
    20941 aataatggtg atgaagtaga agttttagat gtgttaaaaa ctggatgggt aaaaataaaa
    21001 tacaatgaag gtattggata tgttagtggg tcttatttga caaataataa gcctgataat
    21061 agtaatgaaa atataaaaat aaagtatgta aaagaaaaag atggtctaaa tgttaggaaa
    21121 ggaccaagta cagaagatga aaaaataggt cacctttcat atggaagcaa agtagaaaca
    21181 atagaaatgt ttgctactgg atgggtaaaa ataaagtata atggtggata tggctatgta
    21241 agtaatgatt acttatctga tacaccagtt atttaattat aagtaacaat aaaaaaactg
    21301 ctttaatttg taaagcagtt tttttataaa aatttaagtc taataactat ttctattttg
    21361 ataccttaat cctgggattt attattatgg atattatcaa catacaaaca ctccaaatcc
    21421 agtgtctaat tccaaactta gatattaata aattcagtcc tgctatggta aataagcagt
    21481 atttaattac aattaaaagt gtatttatag aaagcccctt catagaaagt atcctaaatc
    21541 caataataga cattctaagt cctataaatg ccaataatat acctgcaata tcctgaatta
    21601 agtaatagta ttcaaatttc atattcaatc aactcttctc tgagcagtat caaatatttc
    21661 taactcttca atttcatcat ctgtgtatgt aagcatagta aaaccagtta caccataaat
    21721 aaatgaaatt ataggtgtaa tccaacttaa gaaacaatat ggaataaatt ctagtgggct
    21781 tacaccaaga gtctgagcac agaaaacagc atttgaattc catggtatta atggaccacc
    21841 aagtgtagct gtatcttcta taattctaga tagattttgt ggttttaatt taaattcttt
    21901 gtatattgga gacattagag ttccgacaaa tacagaagtg aacatcattg atgaactaaa
    21961 tgcatttgca aagtagctta ctataaaagt agacccaacc aatttagttc ttgattttcc
    22021 tattttagtt ataagaggtt gaagcagtac ttctaagaat ccagtttttt ggagaatacc
    22081 agcaataacg aaaactacaa atactagtaa aacggtttca gccatactca ttattccacc
    22141 acgatttaat agtttgtcgg catagacaaa tccagtatct atagtaaatc ctgatagcat
    22201 gtaatttaaa acatcaccta ttttttctcc ttcttgaaat actgctataa taagtcccat
    22261 gatagctgaa cttagtatag atataatggg tggcttttgt agcaataaaa gtaataataa
    22321 aaatattatt ggaatcattg ctactaaacc aatatgaaag tttgaattta atacatcctt
    22381 gatttgattt atttgaacat agtctatagt attatttgag tacttaaatc caattactgt
    22441 atacagaact atacatatta cgtatgcagg tcctgtggta taaaacatat gcttcatatg
    22501 tgtaattaca tctgtcttac aaactgctgc agctaagttt gtagaatctg atagaggtga
    22561 catcttatct ccaaagaaag caccagaaat gactgcacca gctgttaaac caagaggaat
    22621 acccatacta gtaccaatac tcatcattgc tataccagaa gttccaagag tcccccaaga
    22681 tgtaccagta gcaacagaag ttaatgaaca tacgattaat gttgtaagca gaaaatattt
    22741 aggggttatt attgttagtc cactatatac tactgttggt attgtaccag cagctatcca
    22801 agctgctatt agtacaccaa cagaaagtat aattaagttt gattgaaagg catctttacc
    22861 aacttcaaat ccaaaagctt ctatttcatc attagtataa ccaagtttca tggctgcagg
    22921 aactactata agccatgaaa gtaaaaacat tgtagttata gaagcattaa atacaactat
    22981 accaactgat gttagagcta ttattgcaag cattactaac aaggaatatc taaatgaagg
    23041 tatttttctt tctgtattca ttttcatagt gcaaattctc cttaaatatt tttagttaat
    23101 tgcatttttt attcttataa gagcttcttc aagcatactt cttggacaag ctaagtttat
    23161 tctttgatac ccacttcctc caatgccaaa gctgtttcct tgatttagag caactttacc
    23221 tttttgtaca agtatacttt ctagttcctc atcacttaat ccaagtgcac taaagtctac
    23281 ccataataaa taagtacctt ctggttttct aactttaagt tttggcatat tttcatttat
    23341 atattttata gcaaaatcta tattagattc taaatattct aaaaatgatt ctagccaaga
    23401 ttcaccatta ttatatgaag cttcagttgc aactaagcta aagcaattat ttctctttat
    23461 atcaattctg gtaaaggcat catctaacaa tttatagtct ttttcatcag gtagtattac
    23521 atatgatgat tgaagaccag ctatattaaa agttttagtt ggagccatac aagtaattgt
    23581 atttttttca aattctttag aaatagatgc cataggaata tgcttatgtt tttttaatat
    23641 aatgtcacta tgtatttcat cagatatgat ttttacatta tgcttaagac aaatatctcc
    23701 tagttttttt aattcatctt ttgtccatac tctaccaact ggattatgag gattacaaag
    23761 tatgaataat tttacatctt taatcttgtt ttcaatgtct tcataatcca ttatgtagtc
    23821 tccattttct agtttttgta atgggcttat aattagttct ctattgttat tttttacaac
    23881 actattaaaa gggctgtaaa ctggttcttg tatcattatt ttatcatttg cttttgtgag
    23941 ttcatttatt aaaagactta tagctgggat aacacctgga ctataaatta accactcact
    24001 ttttattttc cagttatgtc tcctatctag ccagtttact atagattcat tgtaagaatc
    24061 tggtcttgtt gtgtagccat aaatctcttg ttctagtcta ttttttaagg aatcaattat
    24121 acaaggtgca gccttaaaat ccatgtcagc cacccacata ggaagtaaat cattagttcc
    24181 atactttttt tccatttctg accatttaga tgaaaaatta ttgcttctat caacaatctc
    24241 attaaaatta taattcattg taaatacctc cagtaatttt aattttatgt taataatatt
    24301 aagcaaacaa cgtgccaata ttgataataa aaaatattta ttcacaagtt aatatttaat
    24361 attaagtaat ttcagagcat agataagata aaaatattaa ttatacttaa aactttgtgg
    24421 aaaaggttat ttctaataaa tttttaaata tatgaaaatg taattaaata ggttaaatag
    24481 gtttaactgt ttaacttgtt agacctattt atatttttta gaaattctac tccttttgat
    24541 gttataattg aacctccacg accaacaagt atgtctacaa gcccaaagtc ttgtagttct
    24601 aaaagcatat ttctaatctg ttgttctgtc aaaaatctat tttcctctaa agctatttta
    24661 cataagcttt ttctacctat tctttctttt aaatcataag ccttttttaa attatccaat
    24721 ataaatatgt attcatctag tgacctttta aaattatagt cgtatttcat gatgtttttg
    24781 tctttgccaa tgttgaattg atactttttt atattatcac ttactttatt acaaacggtt
    24841 ctactataat ttgaatctat ggtatctaat atatattcag gtaaatcaca tatttctata
    24901 atatcttttt ccaagtaaca gaagtattct cctaagtttc ttaattctct tacatttcct
    24961 tcccatctat acattttaaa gatttctttt acttcctcac ttagaataaa attacatgga
    25021 atatcatatt ttaatgaacc aaaaatttca aatatatcag caggtctttc cctaagtgga
    25081 tgtattttaa ggggaagaac attcaaccta aaatataaat ctcttctaaa catattttta
    25141 gatacaagtt cttttaaatt tctattagta gctgcaatta ttcttacatc aatgtcaatg
    25201 acattattag aaccaatacg tcgtacttgt ttttcttgta tgactctaag taatttagat
    25261 tgagaattta aatccatttc tccaatttcg tctaaaaata tagttccatt gttggctaat
    25321 tcaaataaac caattttccc acctttttta gcaccagtaa aagccccttc atcatagcca
    25381 aataattcac tttgcagtag attttcttga aaagtactac aatttactgc gacaaaagga
    25441 ccatcttttc tcctagaagc actatgaatg gattgagcaa ataattcttt accagtacca
    25501 ctttcaccaa ttataagtat agatgaattt gattgagcca ttttattagc aatttttttt
    25561 gtattaatag tttgtatact agaacctaat atatcatcaa atgtgtattt actgatatta
    25621 ccagagttta ctagttgtgc tcttaattta gcttgctttt tttctaattg agaaaacttt
    25681 gtcatcttta gtataaatcc atcaaataca tttaattgta tatgtttaat ctctaaattt
    25741 atatcaatat gatttatttt aatcaatttc tcaaaaaatg gagttttatt ggtcaatatg
    25801 ctttgaaaat tgatgtcttt tatgcaatca ccaacaaatg agtcaatcat ttcatttgca
    25861 tttatagaca aaatttttct agccatatga ttatagaatt gaattattcc atcgttattt
    25921 gtgcatataa ttccatcatc aattatagat agtaacaaat caaattgtct ttcaaactta
    25981 tttgcatcta acaataactc acctgtacta taacttgtag gaattatttt ttctacgtat
    26041 ttttttatta ggtcatcttt tattagatgt tttaatttgg tttttgcagc aatttcaaca
    26101 atacaactta aatctattat tctgtatttt aaatctataa cttcacaatt tttaaactta
    26161 ggcaagattt tttcgccagg agttaggatt acagcatctg tgggagtaaa ctcaatctct
    26221 ggatagcagg gcaggatatt tatattgtct attcctaatc tatgtattaa agaaattgtt
    26281 tctattgcca tatctttact taagttatat acataaataa ttttatcttt ttctaatttg
    26341 gatatcatat caatactgtt cttttcaaat gtcaacttgg gaacaatgat ttgagcatca
    26401 tagtgagata attttactat ttcatcatat tttaaataag cagataggag tataagtttt
    26461 tctttaaaaa actttaaatt tccctcttca aatgagtata aatttatctt tagattatca
    26521 cctaagaatt gccttatttg attttcataa atttctccag cgtctttttt taaacttaca
    26581 atagctaatt ccatttgttt cattttaacc tctcatacaa atttgactta ttttagatta
    26641 cataataatt aaattaaata atattattta attattatta taaatttatt atagactaca
    26701 cattgaagta aaagaagtag ctaaaattat tccttttatt tttgtttttt gtatctattt
    26761 ggtataatag tattagagcg tataataatt taggcaaaat tttttaagga agactagagg
    26821 agaatataaa atgaaaaaat gtctttactg taatagggat ataaagtcac caaataataa
    26881 attagctata aaatatgatg acaatatgaa tatatatcca tgtcgtgcag aatgtaagga
    26941 aaaaattgaa gaatatatag caaaaaaacc tacatataag tttataaata tattagaggt
    27001 tcttttaggt gtgggcggaa tattctgctt tttgagtaaa tggactattg cagcccaagt
    27061 tgtacttgga attgatgcat tggttatatt tttattccca ttggcaggat ttaagatgaa
    27121 tggaaagtta agtatggaac aaactaagaa tcaaagtagg tctattgcaa tatcaatttt
    27181 ggcatttata gtaataataa ctgtattata ttttaaacaa gttggtaagt agagcataat
    27241 tgtataagac atcattagag tatgaggtga gtttatggaa gaaagaaaat tagcaccaaa
    27301 caatgagagg gaagctctca taaaaagatt aaaaagaata gaaggtcaag ttaagggtat
    27361 acaaaagatg gtggaacaag aacgttattg tgttgatata ttggttcaaa tatctgctat
    27421 aaggtctgct ataaatagag taggcacaat tatcttagag aatcatataa aaggatgtgt
    27481 agcagaaagc attaaacatg atgatgctga taaaactgaa gagatgataa aagagcttat
    27541 ggatactata aataaattca caaaataagg aggcggttag ttatgaatca acaaggattt
    27601 attatggaaa ttgttgatga tagaactgca aagttaaaaa tgcaaagaca ttcagcttgt
    27661 gctgcttgtg gaaaatgtgc tactacaaat tcagcagagt caaaagaaat attagtagaa
    27721 gtagacaaca caattggtgc aaaagttggt gaccatgtag aagtgagtat ggataatatg
    27781 aatgtattaa aagcagctgt aatggcttat ataatacctc ttatagcact tcttgtagga
    27841 acaataggga cttattatat attgggggca attggaatga caactggagt tgaagccata
    27901 agtggattag ttggaatttg tcttacaata atctgttatt tatacttaaa gaagaatgac
    27961 aaaaaattta gagacagtag agaatttata ccagtaataa caagaatatt aattgacctt
    28021 taagataatt ataattaaaa aatagcaatg tgctttttaa ataaatactc aatacgtatt
    28081 tgtagagttt aagtacattg ctatttttat atttttattt tgattttttg taaggaataa
    28141 agttttttct gattttcaaa agattcaatg ttcttacaaa agaagttaca actaatacac
    28201 caagtgctat tgcagcactt agaaatagcg catctgtacc ttttgacata gccaaagaat
    28261 aatttttctg aatcaaagac aacattgtat tatatagccc aattcctggt attagaggaa
    28321 gaatacctgg aattacgaac acaatagctg gttgctttag ttttcttgct aaagtctcac
    28381 tgcacgctga gaccaaagca gcagcgataa aacctgcaaa aactgcattt gtagtaaaat
    28441 taaataaata aacatataca atccaaccaa ttccgccagt tactccagct ggaagaagta
    28501 gatatagtgg agcattaaag aaaacagcaa atccagcagt cgcaacacct gaaaataaaa
    28561 aatgcaaaat catagacata tcagtcatta gaaaggacct cctattctat accatgtatc
    28621 aaggacaaaa ccaactccag aagcaattgc aataataatc atagcagcat caaaagcacg
    28681 agaaattcct gatattaaat tccctgatat taaatctcta atagctttta taaaagcaac
    28741 tcctgggagt agtggcatta tagcgccgac tataagcatt ttagggtcat ctaaaatgcc
    28801 tatttcaact agtagaactc ctgaaagtgc aattaaaaaa gaagatacta aacaggaaaa
    28861 tgcagatatt gcacttatct tcatcgtttt atcataagta attgcagcaa agattgctat
    28921 tataaacgta agtataaaat ttaatacata attccctcca agcaaacaag caaaacaagc
    28981 agatgcaatt cctgtacaag agtaaaataa ccataaagga taaggtttta catcttggat
    29041 ttcataaaga cgtgcaatgg cattatcaat atcatactct ttgtcactaa caaattctcg
    29101 agaaaaactg tttaataaag aaatcttatt taagtttata cctcttgatt gaatcgtttt
    29161 cataaacgaa aagccatcaa atttctcatc tgaaataatt attactgtag gagttgtaaa
    29221 aatatttaca tgataaaacc ctcttgacct gcatattcta aggacactgt cttcaaccct
    29281 agatgtttca gctccatttt ttatcatcag ttcccctaca aatagagcta gttttagaat
    29341 atttttctta tattcaagtt cctcattttg attcataaat acacctccaa attaaaaaac
    29401 gtaatattga aattatatca aatttttaag taatattcct taatatctat ataaatatat
    29461 tatctttttt ttgtttcaaa atagtcacat ttaaattata tccaattaaa agaattagac
    29521 aatattattt attatatttt aacttaaaaa taaagaaagt aactatgtta aatatggaaa
    29581 aatggcaaaa cacttgacta ttaattggta aaggactata ataataaatt atcatattga
    29641 taaaaacgaa aattatgaaa tgtctgtaaa aataaaattt aaataataac taggaggagt
    29701 aatatgtttg agaacttgaa aaaagcggac cctgagattt atgaaagtat gaaaagagag
    29761 ttaaaaagac aacaaagaaa tattgaatta atagcatctg aaaacatagt atcagttcca
    29821 gtaatggaaa ctatgggaag tcatcttaca aataagtatg cagaaggtta ctcaggcaaa
    29881 agatattatg gtggatgtga atacatagat gaagttgaaa ctcttgcaat agatagaatc
    29941 aaaaaaatat ttggagcaga acatgctaat gttcaacctc actctggagc aaatgcaaat
    30001 attggtgtat attttgctat gttaaaacca ggtgatgttg ttatgggaat gaatttatct
    30061 caaggtggac atttaactca tggtgctcca gttaatattt ctggtcaata ttataaattt
    30121 tatgaatatg gtatagataa agacagtgga cttatagatt ttgatgaagt tagaaagata
    30181 gcacatgaag taaaacctaa aatgatagtt gcaggagcaa gtgcatatcc tagagaaata
    30241 gattttaaaa agtttagaga gatagctgat gaggttggag cattgcttat ggtagatatg
    30301 gctcatatag caggtcttgt ggctgctggt cttcaccaaa atccatgtga agtggctgat
    30361 tttgttacaa caacaactca caaaacttta agaggacctc gtgggggtgt tattctttgt
    30421 aaagaaaaat atgctaaaga catagacaaa gcaatattcc ctggtattca aggtggacct
    30481 cttgaacata taattgcttc taaggcagtt tgctttaaag aagctttaag tgatgagttt
    30541 aaagaatatc aggtacaagt tgctaaaaat gcaaaagcct tagctgaaga acttattaaa
    30601 agagatttta aattgatttc tggaggtaca gataatcatt taatactatt agatttgact
    30661 aataaaaata taactggtaa agcagcagaa aagagattgg atgatgctta tataacagct
    30721 aataaaaata caattccatt tgacccaaat ggtgctcttg ttacaagtgg aataagactt
    30781 ggtactccag ctgtaactac tagaggtatg aaagaagaag atatggcaat aatcgctgag
    30841 gctatagacc tatgtttaac ttatgatgaa gaatctaagg ctagaacatt ggttgttggt
    30901 ttaactgaaa aatatccttt atatgaagaa tataatatta tggattaatt ataaagaaca
    30961 actaaataaa atacatttaa ataattacaa tgattgtgta tgtatttgaa tatatttaga
    31021 tataaaaaag gtgtctcaaa atgaactttt tagtttatga ggcacttttt tgtatggaat
    31081 cgaacatatt tgtattattt aaataataaa aaatatggtt atctctattt ttagatggtc
    31141 tctttgtatt ttttaattaa aactttaaga tttcttaaga attatactag gtttttctaa
    31201 agatttaaag tctatactta taagtatagt aatcatatga aaagaaaagc tttaacgagg
    31261 agggaatctt atgaaggtat taattcttac aggaaaattt ggtatgggtc attactcagc
    31321 atcaaattct ttaagtgaag atataaaagc aaaatttgat aattcagaaa taataattaa
    31381 agatatattt gagtacatta tgcctaacta ttcagataaa atgtacaaga ctttttctat
    31441 tcttgtaaat cggggaagta gtctttataa tttgttttat aaatgtgctg aaaatggaaa
    31501 gaaagatata aaatttactt tttcagatta ttttttaaat aaattagata cattacttca
    31561 tgaagtacaa ccaacagtag ttatttcaac ttttccattt tgttctcaat tagtatcaag
    31621 atataaggaa aaatataact caaatcttcc tttaataaca tgcataactg atataagtag
    31681 tcactcagaa tggattagta aaaatacaga ctgttattta gtagctagta aatctactaa
    31741 ggaagaactt gtttttaaag gaatagatga atctaagatt aaagttaatg gtattccagt
    31801 aaaaaaagaa tttaaaagaa tagaacatgt aaatcattca actaaaaaaa atattcttat
    31861 aatgggtgga ggattaggat tactgcctaa atcagaacaa ttttacaaag aattaaatag
    31921 tttagaagga gtaaaaacta ctgtaataac tggtaataat aaaaaaatgt actataaact
    31981 atatggtaga tatgaaaata tagaggtagt aggttacacc aatgaagtat ataaatatat
    32041 gaaagattct gaccttatta tttctaaacc aggtggaata acactatttg aaacaattta
    32101 ttcagaactt ccaatattgg catttaatcc atttttacaa caagagatag ataatgctag
    32161 ttttatatta aataatgaaa ttgggagaat acttggtaaa aataaaaaat actatgtaga
    32221 tgagataaaa gatttaattt atgatgatgc tactcttaaa gaaatgagca gtaacatgaa
    32281 agaattaaaa aaacaatttg ataataatac cttagagaat attttatttt cacttgatga
    32341 acaaggagcg tgtagagaat gtatgtagta ggtttaatta tcgttgttgc attgattttt
    32401 ttagtccatt caattatacc tacatactat aataaactat taaataaaaa ggtattaaaa
    32461 aatatggctg gagaaaatga aattgcactt acatttgatg atggtccaga taagagatat
    32521 acagaaaaat tactagatgt gttaaaagaa aatgatatac aagctatgtt ttttgtagta
    32581 gcaaagaatg ctgaaaaaga gcctgaaatt ataaaaagaa tgttgagaga aaatcatata
    32641 gttggtttac attcattgga acacagaaat gcatggcttt atagttatag ttatgttaaa
    32701 aaagatttta ttgaaagtac aaatataatg aaaaatttag gtgtagatgt aaactactat
    32761 cgtccaccat ggggtcatac taatatattt tcaaattcat ttgttaaaaa atataatcta
    32821 aaaatgactt tgtgggatgt aatggcagaa gattgggaga aagatagtac agtagacatt
    32881 attataaaca agttaatgag tagaacaaaa gaaaattcta taatttgttt acatgatgca
    32941 ggtgaaaatt caggtggtgc agtaggtgca cctgagcgta ctattgaagc tttaaagata
    33001 gctattccaa aacttaaagc cagtggttta aagttcgtaa caccagaaag gatgtagtta
    33061 tgataagtaa aactgaaaag aaaaataaat gcctaggtag tgtagctttt ttagttttac
    33121 taatgggcat aacaggatat tttgttttta gagggcagtc agttgaatca ttgataaaat
    33181 cattaaaagg tgcaagccct atgtttatac taattggatt tgcaatgatg tttatatatg
    33241 ttgcctgtga aggtataaat atatacttag gtatgaaagc tttaaaccaa aagactactc
    33301 ttttaaaatg tatgggatat gcttttattg gattttattt tagttctatt acaccttcag
    33361 catcaggtgg tcaaccagca caagtttatt atatgaaaaa agacgatata aatatatcat
    33421 attcttcact tatactatta gtcattgttg ttattcacca agtagttata ctggcatata
    33481 gtggaataat gttcattatg gaacgtgagt ttatattaaa taatgtaagt ggtatgaaca
    33541 ttttactaat atatggtgta ataacaaatg ttgctcttgt cattggagta attgcaatta
    33601 tattttcaaa aaaacttgta aacaatttta taatatcaat aacaaattta ttaggtaagt
    33661 taagaataat aaaagatgtt gaaagttcaa gaaaagttat aatatctcaa attgaagagt
    33721 atgttaaagg tgctcaatat ataaagcaga atccaaaact tgtagtccaa attttagtca
    33781 taactattgt acaaataaca gctatgtttt tagttccttt ctttgtatac aaagcttttc
    33841 atctatcaac atatactgta tttgagatat tagcgataca atccttgctt aatattgctg
    33901 tttcttcatt accattgcca ggggctgttg gagcttctga aaatagtttt atgacattat
    33961 ttaagatatt tttccctggt cacttattag ttccggcaat gttacttagt aggggaataa
    34021 gtttttatgc atttgtagct ataagtggat tgatatgtat agtagtacat gtaaaatctt
    34081 ctgaaaaagt aaaagcagtg aaaaaagttg cttatgtaag ataatattat atagattatt
    34141 tttattaata tgtcaatttg tgtgtatatt ttaattaagc tttaaacatg tttacaggag
    34201 tagaaactat taaaacgtgt gacattgcta agcttttgct aataaagtat aatattttat
    34261 atagtttgaa atatttaaga aatatagcaa aaaaaggagc ttggtgatga ataaagagat
    34321 tccaaaaaaa attttagtag ctgatgataa tcctgagatt agggaaatcg tagaaatact
    34381 tttaactggt gaaggttatg aagttattat ggcaacgaat ggagaagaag ctgtaaactt
    34441 agtagatagt acgattgatt tgattatttt ggatgttgta atgcctgtaa aaacaggatt
    34501 tgttgcatgt aatgaaatta gagaaaaaac gacagcacca atattatttt taactgcaaa
    34561 aactcaagac tctgataaag ttattggatt ttcagcagga ggagatgact atttatcaaa
    34621 gcctttttca tattctgaac taatttcaag ggttaagtcg ctacttagac gttattatat
    34681 ataccaggga aaggaaaagg aaagagagcc aaatatatta aaaacaaaag agttatgtgt
    34741 caatttagat actcaagatg ttacactaaa tggagaacct gtgtttttga caactataga
    34801 atatagtata ttaattctta tgttgaaaaa tagaaaaaaa gttttttcgt ctgaaaattt
    34861 atatgaaact atttgggatg agcagtattt ttatacagca aataatacaa taatggttca
    34921 tattagaaat ttaagaaaaa agttagaaaa agaccaaaaa aatccacaat atataaaaac
    34981 cgcatgggga aaggggtatt atattgatta acaaaattaa gccatctaag ttgggagtta
    35041 tactattgat atatgtagta atctcagctg cagtggcttt cattttaaat ataccatttt
    35101 actctgctgt tgaatctata gtaaaagatg tatcagaagg gaaagtatat tttaaaagag
    35161 aagtaaatca acttatggat aattttcaaa cttatgtgaa taacaatggt ataagagcag
    35221 atgatggaga tgatatagca aaatggaata gtgataattg gtttgtttct atgaagatat
    35281 ataaaggaaa taaggttttt tatgatacat tatactatcc taatgaagta cctgataaga
    35341 gtgaggaaaa tgagttttat agcagtccca tggctagaaa ctatgagata caattttctg
    35401 atattaaagc taatatagtt gtagtagctt tatttagatt tagatttcaa aattttatag
    35461 aagcaatttc tattacattt atggcactta catttattat aacattttta ctgttgttta
    35521 gtagaaagat aaagtaccta aggaaaatag aaatgggtat aaagatagta gagagtggca
    35581 gtttaaagta tagaatccca gtaaaaggta atgatgaatt atcatcactt gcatctagta
    35641 ttaatgagat gagtcataca ttggagaatc aacttataaa agaaagggag atgaagaata
    35701 aaaataaaga tatagtaact tcgatatcac atgatgttag aactccatta acgtcagtaa
    35761 tttgttattt agatttgatt aaagaccata aatacaatag tcctgagcaa ttggaacaat
    35821 acataaaaaa tgtaaggtct aaagcatatc aaataaaaga tttaacagat gaccttttta
    35881 cgcattttgt taattctgga gaggaagttt caaaagggta tgaaaaaata aatgctaatg
    35941 aatttatttt tcaattactt ttagatagtt catatacatt ggaggagaaa ggatttcata
    36001 taacaataga aaatgaactt tataaccaat atcatattta tgtagatgtt acacaattta
    36061 gaaggatatt tgataattta tgttctaata taataaaata tgctaggaaa gataagccta
    36121 tagtattttt aattaaagta gaggattcta aattactaat tatacaaaaa aatagagtaa
    36181 gtaatttttc taagaatgta gaaagttttg gaattggtat aaagaattgt gagaaaatag
    36241 ttgcatatca taatggaagc ataaatgtaa attcagatga taaagaattt gctattgaaa
    36301 tatctattcc agcaaaagaa tcataaaaat ttaaattata tattgtgtag ttgctattat
    36361 atattgtgta attgcttatg taaaattcta tgaagcagga agcatgtgac tttagttatg
    36421 ggatgttcaa tattgtgtac ctctaggcaa gtaacaagtt tacttacaag accatataat
    36481 attattatct taaaaaaagg ggacataata atatggtttt gacaggagca cattatattt
    36541 acattctatt tatggtaatt atattaatta ctatgattat gaaaaaggat acaatagtac
    36601 catgtatatt aggtgttttt tttatgggat tgttctttga aaaaaacatt tttggtgcta
    36661 taacagcagt gtttaactct tttattatat ctttaaatga gttgggtcca attatcttga
    36721 ttatagctat tatggttgca ttatctaaag ccctagaagc caataatgct atacaatata
    36781 tggtgagacc attttcgaga gttataaaaa attctaatac agcatttttt gtaactggat
    36841 ttgttatgtt agttctttct tggtttttct ggccaactcc agctgttgcc ttagttggag
    36901 ctgtattttt acctgttgct atgagagctg ggctaccagc aattggtgtt gcagtagcat
    36961 taaatttatt tggtcatggt cttgccttgt ctacagactt tgttattcaa ggagctccaa
    37021 gtataacagc tggtgcagca ggtgttgcag tatcatatgt tataaatgat ggtatgattt
    37081 tgttttgggt gatgggaatc gtaaccatat ctatggcatt ttatacatta aaaagagata
    37141 ttaacaaagg aatgtttaga gaagaactaa aaggttttga aagtgaagaa gtagaagagt
    37201 tcaatggaaa atctaaaata gcaactattt tagtagctct gggatttctt gctgatataa
    37261 ttgctatgta tgtatttgac ctaaaaggag gagatgcatc agctcttttg ggtggaacag
    37321 cagtattttt aataataatt ataaatgtta taaattttgg caaagatagt cttgaaaatg
    37381 tatgtgagaa tataattgat ggttttgtgt ttgggataaa aatatttgga gcaattatac
    37441 caatagcagc atttttctat atgggtgaag ttgcaccatt gacaggtgta tttggaaaag
    37501 tattagcacc aggttcacaa ggtttattat cagatatagg catagctctt tctcaaacaa
    37561 taccacttaa taaatttgcc gtatcaggta tagaaactgt agtgggagca ataactgggc
    37621 ttgatggttc tggattttct gggatttcat tagttggttc acttgcctct gtatttggaa
    37681 ctgctataaa tgcaagtgtt ggtgcattag ctgcattagg acaaatcagt ggtatatggg
    37741 taggtggagg ctgtctagtt ccttggggtc ttatttcagc agcggcaatt tgtggggtta
    37801 gtccaataga attggcaaag cgaaactttg tcccagttat aaccggattg gttgtaacaa
    37861 ccatagtagc aatgtttatt atttaataaa tcgaataaat ctttaattgt ataaattaaa
    37921 aatagatgtg tagctataat ttattataat tgttttacta attctaagcc atacatctat
    37981 ttttattgat atttttatta gtattcttta ataaagtaat atttttcatt atattataca
    38041 taacatctaa atataacatc taaaatactg ttatctatat ttaatatctt gccaaatttt
    38101 ttgatgtttt attgccctgt cccaccaaca tatataagct atcacaagtt tttacctata
    38161 ctatcaccaa acaaaaattc taattttaat ttattatttt ataacctata ttatattaac
    38221 tatattagtt tgtagacttt agatgtaatt ataaatatta atactccaag agttcaaatt
    38281 catatcctag ttttcttgca tttgatataa aatcatttaa aatttctgta ctagttttgg
    38341 aaactgcatg tagtaacaag atagaaccat catgtagatt atccattatt ttttgcgttg
    38401 cttcttcatg tgatggttgt ttatcagtat cccagtctct gtatgcaaaa ctccaaaata
    38461 cagtcttgta tcctaaattt ttagtcattg ccaagctttt ttctgagtat tttcccatag
    38521 gaggtctaaa aaatttaacc atatctttcc cagttacatc tttatataac ttttcaacat
    38581 catataattc gtcattaaag ttttttagat tgcttgtttt tgttggcata gatggatggg
    38641 ttttactatg atttccaact atatgtccct cagaaaccat tctttttact aaatctttat
    38701 tttcttttat atatggagct gtaacaaaaa aaactgcttt aacctggttt tgttttagag
    38761 tgtcgagtat ttttgtagtg tatccatttt cataaccttc atcaaatgta agatatagtg
    38821 ttttttgacc atcttttgta ggtccattgt atagggcatc atagtcagaa aatttaaatt
    38881 caatgtccgt atttacttct ggtgttttat gcttattgtt tggtatataa aaccaatcaa
    38941 gagtagtgtt atctaaagaa gatacatcta atttattatt atcattatta gtagtggctt
    39001 ttgatggttt atctgttccg tctgaaacta ttacattgga agtatcttta ccactatcta
    39061 ttttttcctt atcttcttgt aactgtgttt ccttattttg attttcgttt tgattgtttt
    39121 gtgaatttga acatccaaca ataaaaaaaa tagaaataga aagtagtgtt aaataatgca
    39181 atacttttct aaacattttg aagctccttt ctaaattaat aataattgca tataataata
    39241 attatatgat taattacaat tgcatatagt attattataa ttggaaatta ctattaataa
    39301 tagttatatc tttaaaaata aagtcacaat agtatgacaa agggtgaata atcaataaat
    39361 ttaaaaaaaa atgaaaaaaa tagttgacca tattatgaga agatgttatt ataataaatg
    39421 tgctcaacaa gaagtaacaa aataacaagc acaaagaagt taattgaat
//
LOCUS       DAEMIK010000019        38369 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEMIK010000019 DAEMIK010000000
VERSION     DAEMIK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.019"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            535..1719
                     /locus_tag="KPK87_000833"
     CDS             535..1719
                     /locus_tag="KPK87_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537008.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(2096..3205)
                     /locus_tag="KPK87_000834"
     CDS             complement(2096..3205)
                     /locus_tag="KPK87_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2537009.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3668..4861
                     /gene="nifV"
                     /locus_tag="KPK87_000835"
     CDS             3668..4861
                     /gene="nifV"
                     /locus_tag="KPK87_000835"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG2537010.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4886..6811
                     /locus_tag="KPK87_000836"
     CDS             4886..6811
                     /locus_tag="KPK87_000836"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG2537011.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6833..7828
                     /locus_tag="KPK87_000837"
     CDS             6833..7828
                     /locus_tag="KPK87_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG2537012.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            8154..8627
                     /locus_tag="KPK87_000838"
     CDS             8154..8627
                     /locus_tag="KPK87_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2537013.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8661..9155
                     /locus_tag="KPK87_000839"
     CDS             8661..9155
                     /locus_tag="KPK87_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2537014.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            9234..9773
                     /locus_tag="KPK87_000840"
     CDS             9234..9773
                     /locus_tag="KPK87_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2537015.1"
                     /translation="MKGMIYMIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSK
                     GLRTWEFIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAA
                     SYIQLQAHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEE
                     KKAYSDSDLDYKKVHFNHF"
     gene            9962..10273
                     /locus_tag="KPK87_000841"
     CDS             9962..10273
                     /locus_tag="KPK87_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2537016.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            10301..10987
                     /locus_tag="KPK87_000842"
     CDS             10301..10987
                     /locus_tag="KPK87_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2537017.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLFLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            11219..12103
                     /locus_tag="KPK87_000843"
     CDS             11219..12103
                     /locus_tag="KPK87_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG2537018.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            12202..13377
                     /locus_tag="KPK87_000844"
     CDS             12202..13377
                     /locus_tag="KPK87_000844"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG2537019.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            13505..14359
                     /locus_tag="KPK87_000845"
     CDS             13505..14359
                     /locus_tag="KPK87_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537020.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            14403..15638
                     /locus_tag="KPK87_000846"
     CDS             14403..15638
                     /locus_tag="KPK87_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG2537021.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            15739..16680
                     /locus_tag="KPK87_000847"
     CDS             15739..16680
                     /locus_tag="KPK87_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG2537022.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGIDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(16899..17252)
                     /locus_tag="KPK87_000848"
     CDS             complement(16899..17252)
                     /locus_tag="KPK87_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG2537023.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLCAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDDLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            17548..18459
                     /locus_tag="KPK87_000849"
     CDS             17548..18459
                     /locus_tag="KPK87_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG2537024.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            18720..19154
                     /locus_tag="KPK87_000850"
     CDS             18720..19154
                     /locus_tag="KPK87_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537025.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            19177..20712
                     /locus_tag="KPK87_000851"
     CDS             19177..20712
                     /locus_tag="KPK87_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537026.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            20796..22163
                     /locus_tag="KPK87_000852"
     CDS             20796..22163
                     /locus_tag="KPK87_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2537027.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            22198..22422
                     /locus_tag="KPK87_000853"
     CDS             22198..22422
                     /locus_tag="KPK87_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG2537028.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(22872..23270)
                     /locus_tag="KPK87_000854"
     CDS             complement(22872..23270)
                     /locus_tag="KPK87_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG2537029.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(23273..23674)
                     /locus_tag="KPK87_000855"
     CDS             complement(23273..23674)
                     /locus_tag="KPK87_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2537030.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKYNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            24034..25020
                     /locus_tag="KPK87_000856"
     CDS             24034..25020
                     /locus_tag="KPK87_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537031.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            25004..25915
                     /locus_tag="KPK87_000857"
     CDS             25004..25915
                     /locus_tag="KPK87_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537032.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            26028..27596
                     /locus_tag="KPK87_000858"
     CDS             26028..27596
                     /locus_tag="KPK87_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2537033.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            27671..28696
                     /locus_tag="KPK87_000859"
     CDS             27671..28696
                     /locus_tag="KPK87_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537034.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            28689..29633
                     /locus_tag="KPK87_000860"
     CDS             28689..29633
                     /locus_tag="KPK87_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537035.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKLLSWIEVSDGHFVYSSKSDINKYQQNLKVV"
     gene            29992..32649
                     /locus_tag="KPK87_000861"
     CDS             29992..32649
                     /locus_tag="KPK87_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2537036.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            32864..33493
                     /locus_tag="KPK87_000862"
     CDS             32864..33493
                     /locus_tag="KPK87_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537037.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            33628..33954
                     /locus_tag="KPK87_000863"
     CDS             33628..33954
                     /locus_tag="KPK87_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2537038.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            33970..35259
                     /locus_tag="KPK87_000864"
     CDS             33970..35259
                     /locus_tag="KPK87_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2537039.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLASAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            35283..35612
                     /locus_tag="KPK87_000865"
     CDS             35283..35612
                     /locus_tag="KPK87_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2537040.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKYHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            35760..37088
                     /locus_tag="KPK87_000866"
     CDS             35760..37088
                     /locus_tag="KPK87_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2537041.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            37405..38265
                     /locus_tag="KPK87_000867"
     CDS             37405..38265
                     /locus_tag="KPK87_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG2537042.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRSNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
BASE COUNT        15185 a         3978 c         6825 g        12381 t
ORIGIN      
        1 gttacgttta ttttaagaga aatcaatata cttttatatg aaatattgta aaaaaattta
       61 tttttaatga gaatatattt tcattaataa acaattttat tatgtattaa taatgttaga
      121 ataaatataa ataaatcaaa gttacttaca ttacagtata ttttttcaca aaatatattg
      181 atttattctt ttaaagtttg ataattccaa tgaatgtaat aaaagaagga attttatact
      241 atactattat tattataaaa taataaagaa aaaattatat aataataaag aaaaaatgta
      301 taatatttaa cattaaaaaa gatatttcat aaaattaaaa aacaaagtga atttgaatac
      361 tatttaaaaa ataaaataaa tatataaaaa atatttaata taaaggtaga aataagttga
      421 aaatgtaaag tcattggaaa aatatagttg aatttaatga gcaaaaatgt tattgtttta
      481 tcaataaagt gttaaaataa aaacttttaa tatcatttta gggaggatga tttcatgcaa
      541 aaaagtgtaa agaaagcaaa agtaacgggc tctatgttag ccatttttgg tgttgcaagc
      601 gttcttttta gttcacatgc aggaggagga tttgctacag ggaatcaaga aactcagtac
      661 tatgtgcagt atggatggac agcaccactt atggcaattt tagcaatgat tatacttact
      721 gctaccatga gagaagttat cataatgtat aataataaca attgtaggaa ttataaggat
      781 ttattttgtg agctttggag accatatcca aaacttgaaa taatatggga gatatattac
      841 tatctaatgg ttttaatagc tgtaagtgct gttatagcag gtgcagcagc agtatttcaa
      901 agtataggag taaattattt tgtagctgta tttattattg gagttgtact tcttgtcttt
      961 actatatttg gtgctatgct tgtatctaaa gcagctacag caatgacaat agctattcta
     1021 gtatgtactc ttactatttt tattgttggt ataaaagcta aagtaccaga aataactgaa
     1081 atattatcaa atagaacgag tttcacacct ggctatatca agccaatact taatactttt
     1141 atttatgcag gttttcaatc ggttgttata cctacacttg caggatgttc aagaccactt
     1201 cttaagaact caaaagaagc tactaaggca atgataattt catttgttat gaatgcaatt
     1261 gcattaggac ttgctgtaac tatgctaatg ggatggtata gagaaattat tgctgctggg
     1321 caaacaacac tccctactct ttatgttgca ggtcaatctg gaaatcatac tatatacata
     1381 atttataatg ttgcattatt cctttgttta atgtctacag gagtaactac tatatttggt
     1441 cttgtaaata gatttgaaga ccataaagca ctttctttcc tttcttcaag aatgaaaaga
     1501 agggtattta cagcttgtgc aataatggta gtgtcaatgc ttatatcact tacaggatta
     1561 agtaatattg ttaaatatgg atatggatat tgtggttatc ttggtctatt cactatagta
     1621 attccattcc ttacactagg acattataag aataagaagt ttgcaaagga aaatccagaa
     1681 gctaaatggc cagcggaatt aaatgaaaat gtaaattaga tgtagtagca aaaaatattg
     1741 ctaaagagaa tactatagat aagaatacta tagataatat atctgcatgt ttattaagat
     1801 tatttgcaaa atctaatatt aaacaactat aaacaagcaa tataaaacta agaataaaag
     1861 ataaaaagag ggtgtctcaa aatggacttt tagtttataa ggcactcttt tttatatgaa
     1921 gtcaaatata ttttcattat tttgacaata aaagagacta atcctacttt ggatagcctc
     1981 ttttttcttt aatataaaat agtataacag caaagtatag attgatacat gttttctgta
     2041 gctttctata tattacagtg tttgtgtggc attggtattt taagcaatct ataaattata
     2101 attctataat tcttctacta attttagatt ctatagccgt ctctatttca cttaacattc
     2161 gttcattttt agggtcaata aaagcacatg tatagccttc ttcaccagct ctaccagttc
     2221 taccaatacg gtggatgtag tcttctggtg tttctggtaa atcgtaatta tatatatgac
     2281 ttactccaga tatatcaaga ccacgagaag ctacatctgt agctattaag tattgtatat
     2341 ctaaatttct aaatgatttc attattcttt ctcgttttga ttgagcaata tcactatgaa
     2401 gtttttgaca attatatcct cttatagcaa gagcttcttc aagattgtca acccttctct
     2461 tagttctaca aaatattatt gccataaaag gattatcttc atctagtacc ttacataagg
     2521 catctaattt ttttctatct gtagtttcta ctacctcttg ttttatatta ttaagagtga
     2581 tttcttcttt ttgaatagaa actacaacag gattttttgt gtatctataa gctaattttt
     2641 ttacttgaga atttatagtt gcagaaaagc atagtgtttg ctttttgttt gaagtctctt
     2701 ttaaaatagc ttccacttca tttttaaatc ccataagcaa catttggtct gcttcatcta
     2761 aaacaaaagt ctttaactta ctaaggtcta ttgtttttct atttagatgg tctaaaagcc
     2821 tacctggtgt agctataatc atatgaatat ttccttttag tttctttatt tgagaaccaa
     2881 tatcttttcc tccatatgct gcaagaatac ttacatcttt agattcttta agtttcatag
     2941 cttcttctgt tatttgaata gctagttctc ttgtaggaga aagtataagt acttgtatat
     3001 catttatatc taatgatata ttttcaaata tcggaagtaa aaaagctagt gtttttcctg
     3061 ttccagtttg agcttctgct attacatctt ttccttcttt tattagttta atgctttctt
     3121 tttgtatagg tgtaggtgtt gttattcctg atttttttag aatatttatt gtattttcac
     3181 taatccctaa gcttttgaaa tccattgtta tcttaccttt catgaaaaat tttctttttt
     3241 attaaccaat taataatatc atatttgtaa gtatttttag taaatttttt aattattatt
     3301 tatatagctt aaaaatatat taagatactt taaaatggaa tgtaacttaa attaaatttt
     3361 agtaggaatt ttataggttt atttttaaag gttttttgaa aataaatcta tttaaaaatt
     3421 aaattaaaat gcaagaaaaa cataataact ttcattttta agaagttttc acaaaaaaga
     3481 aaaaatatag tgcaataaat gatacaaagt aatataatag attacaagat atgaaaagct
     3541 aaaaaaataa attgcaaatg aaattaaaat gaataaaaaa taaaaaaata atcataagtt
     3601 agaggtatca atgaaaaata agacttaatg tttttaaaat taaaagtata ctaagggggg
     3661 cattaatatg tgcgttatat caaaagatag agcaaaggaa ataaaaatag tagatactac
     3721 tttaagagat ggagagcaaa cagctggggt tgtatttgca aatagagaaa aaataatgat
     3781 tgctgaaatg ttaagtgacc taggtgttga ccaaattgaa gttggtatac ctactatggg
     3841 tggagatgaa aaaaatgtaa taaagcacat atgtagtaga aatttaaaat cagatataat
     3901 ggcttggaat agagcagtaa taaaagatgt tgaagaatct atttcttgtg gtgttgatgc
     3961 agtagctata tcaatatcag tatctgacat tcatatagaa aataaattga gaacttctag
     4021 aggctgggtt ttagaaaata tggctaaaac agttgaattt gcaaagaaaa atggattata
     4081 tgtttcagta aatggagaag atgcttcaag agcagatata gacttcttaa ctgaatttat
     4141 aaatgttgga aaacaagcag gggcagatag atttagatac tgtgatacag taggagttat
     4201 gaatcctttt tctataaaga atgcaataga aactctttat gaaagaacta attttgatat
     4261 agaaatgcat acacataatg actttgggat ggcaactgca aatgcacttg caggaatagc
     4321 agcaggagca aattatgttg gtgttacagt aaatggatta ggtgaaagag caggaaatgc
     4381 agcattagaa gaagttttaa tggcacttaa gtgtgtttat aaatgtgatt taaataatat
     4441 agatactaga aaatttagag gaatatgtga gtatgtagca caggcatcag gaagaatact
     4501 tcctacatgg aaaccagtag ttggggataa tatgtttata catgaatctg gtatacatgc
     4561 agatggagct ttaaaagacc cacataacta tgaaccattt gacccaagtg aagtaaatct
     4621 tgaaagaaaa atagtaatag gtaaacattc aggaagagct gcagttgtaa ataagttgag
     4681 tgaatatgaa atgtatattt ctccagaaaa cgctaccaag ctattaaatg ctataagagc
     4741 tacatctata agattaaaaa gaagtttaat ggataaagaa attcttcaat tatattgtga
     4801 tatactagca catgagaaag gcacaacaga agaagaagca gttagaggtt catatattta
     4861 agatataatt gtttaggagg aaaatatggg agataatata gtttataaaa taattaaaaa
     4921 acatatagta gatggagaag ctgttgctgg aagttcaatt ggtataaaaa tagaccaaac
     4981 tttaacacaa gattctactg gaacaatgac atatcttcaa ctagaagcta tgggaataga
     5041 taaagtaaag actaaaagaa gtgtggcatt tgtagaccat aatatgttac aacaaggatt
     5101 tgaaaatgca gacgaccata agtatataca aacagtggca gataaatatg gagtttattt
     5161 ctctaagcca ggaaatggta tttgtcatca agtattttta gaaagatttt caacaccagg
     5221 agatacactt ttaggttctg atagccacac acctactgct ggaggtgtgg gcatgatggc
     5281 tattggagct ggtggattag atgttgcttt agccatggca ggaggagcat actatataaa
     5341 agcaccaaaa gtctgtaagg tgaatctagt tggaaaatta aataatatgg tatcatcaaa
     5401 agatataata ttagaagtgt taagaaaaca aactgtaaaa ggtggagtag gtaaagttta
     5461 tgaatatggt ggagaaggtg tcaaaagcct atctgttcca caaagagcta caataacaaa
     5521 tatgggggct gaacttggag caactacttc aatcttccca agtgatgaaa aaacactaga
     5581 attttttaag agtcaaggaa gagaagacgc atggatagag ctaaaaccag atgcagatgc
     5641 agtttatgat gaagagatta ctataaactt ggatgaatta aaaccattag ctgcaaaacc
     5701 tcatagccca gacaatgtag atgaagttga aaatataggc aagattaaaa tagaccaagt
     5761 ggcaatagga agttgtacaa attcttctta tgaagactta atgaaagtag cacaaatcct
     5821 gaaaggaaat aaagtacata aggatgtgag tttggtaata gcaccaggtt caagacaagt
     5881 tatggagatg attgctagaa atggggcttt ggcagatata ataagtgctg gagctagaat
     5941 actagaaaat tcttgtggtc catgtattgg tatgggtcaa tctccaggta ctgactctgt
     6001 gtctttaaga actttcaata gaaatttcta tggtagaagt gggactttgt cagcacaagt
     6061 atatttagta agtccagaag tggcagcagt aagtgcaatc aagggagtgc ttacagaccc
     6121 aagagaattt gatattaaat ttacaaattt agatgtaaat gagtttttga tagatgatag
     6181 catgataatt aaacctgctg atgtaggaag tgatgtagaa gtagttagag gacctaatat
     6241 aaaacctttc cctcttaata cagagctaag tcagtctata ggtggaaaag ttatattaaa
     6301 gacagaagat aatataacta ctgaccatat aatgccatca aatgctaagt tacttccttt
     6361 tagaagtaat ataccgtatc ttgcaaacta ttgtttcaat actgtagata cagaatttcc
     6421 tcagagagca aaagacaata atggtggatt tatagttggt ggagataatt atggtcaagg
     6481 ttcaagtaga gaacatgcag ctttagcacc attatatcta ggtgttaaag gggtaatagt
     6541 taagagcttt gcaagaatcc acaaggctaa cttgataaat agtggtataa ttccaatgga
     6601 gttttgtgat gagaaagatt atgaaaatat atctctttta gataatctgg agataccaaa
     6661 tattttagac aatctaggta gtggaatttt agaagttaag aatacaacta aaggaacttc
     6721 ttttaaggtc aaagtagaat tgtcagcaaa agaagttgat gtattaaaag ctggtggaaa
     6781 attaaactac actaaaaatc aagctaatta atatgaaaag agggaatatt gaatgtataa
     6841 agtgacactt ataccaggag atggaatagg accagaagtt gcgaaagcta tgaaaaaggt
     6901 agtagaagct actggagtag aaatagaatg ggaagaagtt aatgcaggag aggcagttat
     6961 tgaagagtat ggtactccac ttcctgaata tattatagat agtataaaaa agaacaaaat
     7021 agctataaaa ggcccaatta ctactcctgt aggtaaagga tttaggagtg taaatgtaac
     7081 tttaagacag gcattagatt tgtatgtcaa tttaagacct attaaaagtt ttaaggggat
     7141 aaaatcaaga tatgaagatg ttgatttagt ggttgtaaga gaaaatacag aagatttata
     7201 tgctggaata gaacataaaa taggtgatta tgcagctgaa agtataaaga taataacaag
     7261 aagtgcaagt gaaagaatcg ttgattttgc atgtaactat gttaaagata ataagagaaa
     7321 aaaagttaca gctatacata aggcaaatat aatgaaaatg tctgatggat tgtttttaga
     7381 tgtgtttaga gaagttgcat caaaacatgg tgtagaatat gatgatttga ttgtagatgc
     7441 agcagctatg aacttagttt taaatccaga aaattatgat gtaatggtaa tgccaaatct
     7501 ttatggagat atactatcag atttaggtgc tgggcttgtt ggaggtttag gtataattcc
     7561 aagtgcaaat attggtaaag attgtgcgat atttgaagct gttcatggaa gtgctccaca
     7621 aatagcaggt caaaataagg ctaatccaac agcacttatt caatcatcag taatgatgct
     7681 tagatactta ggtgaatatg aaaatgcaca aaaaatagaa acagctttag aaaaagtatt
     7741 tcttgaagga agtaaattga ctgtagattt gggtggctca gcttctacta cagagtttgc
     7801 ggatgaggtg tgtaagtata ttgtatagat tttatttagg gaaaaagtaa aatatagatt
     7861 gaaggaaaga ggctttctca aaatagagat aagccttttt tattggaatg ataaaatata
     7921 tttgatttta tacaaaagag agttcctcat aaacgaaaaa gttaattttg ggatatccat
     7981 tttaatttta aaattaattg attaatacgc aaagaaagat taaataaatt tattattttt
     8041 aattgaactt tcaaccaaaa tatggtaaaa tttaaaaaag ttgaactttc aactaaaaat
     8101 gaatatattg attatgtgaa attctataaa acagaaacta tttgacttta gccatggtag
     8161 tttcagtgga ggtgtgtttg attaagactt tagatagtaa tatattaaga gaagtaggta
     8221 ctttatctag agcagtaaat tcaataaatg atattaagta caaggaatta aagttacaaa
     8281 aaggacaatt tacatttttg acaagaatct gtgaaaatcc tggtataaat cttgtggaat
     8341 tatcaaatat gttaaaggta gacaaggcta caacaaccaa ggctatacaa aaattaataa
     8401 aggcaggata tgtagataaa aagcaggata agtttgataa aagaggatat aatttaactc
     8461 caactgataa atctttggaa gtttatgagt tgataattga agaagagaat agaagtatag
     8521 agatatgttt tgataatttt acagatgaag aaaaacaagt ggtaactaag ttattagaaa
     8581 aaatgagcaa aaacgtagaa aatgaatggt ttaaagtaaa gagatagtaa ataaatacat
     8641 taacttaagg agatattttt atgattagaa aagcgaatat gaatgattta gaaagcatta
     8701 tgaaaattat aaaatctaca gtggaagaaa tgaagactta taataataca caatgggatg
     8761 aaaactatcc attagaaaaa gactttgttt cagatattaa gaaacaagat ttatatattt
     8821 atgaagtgga tggagaagta gcagggttta tatgtcttaa ttatgaagag ccagaagaat
     8881 atttaggctt aaattggtca tctaataaaa aagcaatggt aatacataga atggctgtaa
     8941 atccaaactt tagaaagaca ggaatagcat ctaagttagt tgattttgct gaaaaattag
     9001 ctgtagaaaa caatgtatca tacttaaaga gcgatacata ttcaataaac tcaaaaatga
     9061 atttattact tacaaagtgt ggttttataa aaatcggaga aatgagtttt ttaggaaagg
     9121 agaaatcttt ctactgttat gataaaatat tataaagagc atttacaata caaggggtaa
     9181 ataatagatt ttcagcatta gtaagggcat aatataacct agtgtatgct attatgaaag
     9241 ggatgattta tatgattgga tttctaaaga aaagaagaag tattagaaaa tacaaggatg
     9301 ttgaagtaga aaaagaaaaa ttagataaaa tattaaaagc tgctctttta gctccatcat
     9361 caaaggggct tagaacatgg gaatttatag tggtagatga taaagaaaag ctaattaatt
     9421 tatctcaatg tagaactaaa ggtggtggat ttttcttaaa aaatgcacct ttagctattg
     9481 taattattgc agataaagaa aaaaatgatg tttggataga ggatgcaagt atagctgctt
     9541 catacataca acttcaagct catgaactag gtttaggttc ttgttggata caagtaagaa
     9601 atagaatgca tgatgataat atagaagctg ataagtacat tagagaagaa ttaaaagtac
     9661 ctagcaagta cagtgtagaa tgtataattt ctataggata tcctgatgaa gaaaagaaag
     9721 catacagtga ctctgattta gactataaaa aggttcattt taaccacttt tagtatataa
     9781 tatgttgtat taagacatta tatttttttc tattgttaat atatacttaa aattgtaaaa
     9841 atatatctga attttactaa cttatctagg ggttatgtac aaggatttta aagataaata
     9901 tagaaattaa tgtttataat cgatacataa cttgagtaac aaaatttaag gaggttaaag
     9961 tatgggtata tttaaaacaa aaatggatga ggactggaaa gtaaactaca ttaaagagtt
    10021 taatgaaatg agagattctt atgagagtaa acttcaaaaa aaacagtttg aggtagatag
    10081 tttgaaatca gaattggata gactaagaag ctataaaaat agccttaagc caaaagaaaa
    10141 acaaattact gatgatgata taaataatat aaagagttta agaagagatg gcttaagcta
    10201 taaagaaatt tcaaatcaaa caagttggag caaagcaact gtaagtaggg tattaaatgg
    10261 actttacgat tagcatatac atggggtgat ttaaattggg atgaagtata gttacaaaaa
    10321 atgcataata gattctattt tattgatgtt tatagttcaa atattaagga tgattttgaa
    10381 ttatgtactt ctaagtcaat ttgaatttac tcttgaaaat tttaatatta taaacttaat
    10441 ttcttttaca ttagttggtt tatcgttaat tttattttta aaagataatt ctttgtataa
    10501 caaggttaga aatagaaaaa tcacagaagc ttttgaagaa aataaaaaca atatacttat
    10561 tgaaaagtgt aagctgatac tttttgttgt ggttttattt ttagcaataa ttgtaactta
    10621 ttgtactaag ggatatgtac tttttaatgt aactatgatg actttgtccg tattgatagt
    10681 cccaatattt gaagagttat tttttagaga atatatatgg aactatctaa gtaattttat
    10741 aaagtcaaag agtaaaatta tctgtataac atctatacta tctggaattt ataatatagg
    10801 atacatagat gttataagaa attatgtaat attgtataac aattcgagct acacctttga
    10861 agttattatt tcaaaaatta tgataggtac tgtttttggt attgtacttg gattagttaa
    10921 gtataggttt agagatgtag ggttttgtat attattgagg agcttatttg ctatatttat
    10981 taggtagata gtataatcgg tttataaggt tgttttaaat ttaattttaa atcaacctta
    11041 ttttatttgt gttttataaa gcacttatat attaataact aataaattta ataaaaatta
    11101 tttgaatatt tttaaaatat ataaatatgt attgacatat aaatattatg aattacaatt
    11161 tatataaact aataaaaatt aaataatatt aatcaactta ttgaatggag gaataaaaat
    11221 gaaatttgtg actttttgta gtagcaatga agaaaaaata ggtgtattta attcagaaac
    11281 aaatagtata tatgaaatta atagtttagg attaagtaaa ttatatactg atatgaatga
    11341 ttttattgaa aacgtatcta caggagattt agaaaaaata aaaaacaatt catttgaaaa
    11401 cgcaaagtgt tataagttag aagaagttaa attatgttcc ccaattgtaa gacctaaaaa
    11461 agatattata tgtttaggtc taaactataa agaccatgtc aatgaaatcc cagatggagt
    11521 tataaaaaac gtagttatgc cagattatcc aatttatttt tctaagagag cagacaaaat
    11581 aattggagtg gatgataaga taagcttaca tggagatttg gtagagaagt tggactatga
    11641 atcagaactt gcagtaatca taggtaaaga aggtataaat atatcaaaag aagatgctta
    11701 tgaatatata tttggatata caatagtaaa tgatataagt gaaagagcac ttcaagataa
    11761 acatgttcaa tggtttagag gaaaaagctt agacacacat acatctatgg gaccttgtat
    11821 agtacataaa gaagaatttg aacatccttt aaaattagat ataagtagtg tagtaaatgg
    11881 agaagtaaga caaaattcta atacagagta ctttatattt gatataccta ctataataaa
    11941 tgatttatca agaggaatga ctctaaagcc aggagatata atttcaactg gaactccagc
    12001 aggggttgct atggggatga atcctcaagt atatttaaag catggcgata tagttgaatg
    12061 taaagtcgaa ggaataggag ttttaaaaaa tatagttgat taatggataa atatgccata
    12121 taattttatt agtacgaata atttaattaa tataggatta atcaaactta tgattaattt
    12181 attatataga gggatgagta tatggcaaga gaactagaat taaagtatgg ctgtaatcca
    12241 aatcaaaaac catcaaagat ttatatgaag aatggagaac ttcctataga agtattaaat
    12301 ggaaaacctg gatacataaa ttttttagat gcatttaata gttggcaact tgtgaaggaa
    12361 ttaaaagaag ctacaggtct tccagcagca acttcattta aacatgtaag tccagcagga
    12421 gcagcagtag gggtaccttt atcagatact ttaaagcaaa tatattttgt tgatgattta
    12481 gagctttctc cattagcttg tgcttatgca atggcaagag gagcagatag aatgtcttct
    12541 tatggtgatt ttatagcact ttcagatgta tgtgataagg agactgcaac tattatagca
    12601 agagaggttt ctgatggtat aatagctcca ggatatacag atgaagccct tgaaatatta
    12661 aaaggaaagc gtaaaggcaa ctataatatt gtaaaaatag atgaaaatta tactcctgaa
    12721 cctattgaaa caaaggatgt ttatggaatt acttttgaac aaggtagaaa tgaaatatta
    12781 ataaatgaag acctattaaa agatattcca actgataata aaatttttac agatagtgca
    12841 aaacgtgatt taattatagc tcttattaca ttaaaatata cacaatcaaa ttctgtttgt
    12901 tatgctaaag atggacaagt aataggggta ggtgcaggtc aacaatcacg tattcattgt
    12961 actagacttg caggtaacaa agctgatact tggtatcttc gtcaacatcc taaagtatta
    13021 aacttaaaat ttaaaaagga tataggtcgt ccagatagag ataacactat tgatgtatat
    13081 ctttctgatg attatatgga tgtgcttgct gatggcatat ggcaaaactt ttttgaagaa
    13141 aaaccagagc ctctaactgg agaaggaaaa agagcatggc ttaaaacttt aacaggagta
    13201 gcacttggtt cagatgcatt tttcccattt ggagataata tagaacgtgc aaaaagaagt
    13261 ggagtaagtt ttatagcaca accaggtggt tctattcgtg atgataatgt tatattaact
    13321 tgtaataagt ataacatagt catggcattt actaaaaata gattattcca tcattaaaat
    13381 actgaaagta taaagataat atagattaag taaaaatcta caacaagttt tgttgtagat
    13441 ttttttattt gctgtataat tttaaataga actttatttt tagatattca atttggaggt
    13501 tgatatggct ttaaatataa gaaactatag cctattatgt aatcaaggaa cagctaaatt
    13561 taatgaagat gtagtcggta taaatccatt tggcgcatgg gttttagatg gtgctacagg
    13621 acttaatgga aaaaatttag tgtcaactga aagtgacgca aggtggtatg ttcagtggtg
    13681 gaataggtat ttatataaaa atataaataa agaagaatca ttaaagagta ttattaatac
    13741 tggaataata gaagtaaaaa aagagtatga actaagatta aatgggttta aggttgaaaa
    13801 attagattta ccatctagtt ctatagttgt tgtaaaattt catgaaaata aagtagagta
    13861 ttttttatta ggagattgtg ctttgtttat taaaaatgga gattccaaag ttataaaaga
    13921 taggagtatt tgtaaatttg ataatactgt atttgatgaa atgagtaaac tttctaactt
    13981 ggaagagatg gcttttgatg agattagaac tagtgttatg gatacaatag taagcaatag
    14041 attgaagaaa aatacaaaag agggctattg gatattggat tttgatgagg aagctgtgga
    14101 aaattctata tatggataca ttgaaataaa aaaagatttt caattgatgc taacgagtga
    14161 tggttttaca agtgcatgtg atagatataa tctaataaaa gaagaagaat tgatacgtat
    14221 agcagaaaaa tttggtatag gatatataca taataaagta agggattttg aagataatga
    14281 ctataaagcg gttaaagtgc caagatttaa agtaaaagat gattcatctt gtttatattt
    14341 agatatatat aatgattaga ctgtataaaa ctatttaatt ttagaaagga ggcaagtgaa
    14401 aagtgaaaat tcttcatata ataacacaaa aaccaaatag tacaggtagt ggtatatatt
    14461 tgtctggtat gataaaaggg ttcgaaaaaa tcggtcataa gcaagctgta attgcaggta
    14521 tagatgtaaa tgatgatgta aattgttttc caagtgaagt aagtttttat ccagtaaagt
    14581 ataattgtgg ggagttaaat tttcctgttg taggtatgag tgattctatg ccatatgaaa
    14641 gtacaagata taaagattta aatatagaca tgataaatag attgaaatat caatttaaag
    14701 taaatataga taaggctatg aatgatttta aaccagaact tataatatgt catcatttgt
    14761 accttttaac tgcatttgtt agggaaatgg taaaagatat aaaggttatg tctatttgtc
    14821 atggaacttg tttaagacag ttaaatacca ttgatttaga gaaagaatac ataatagcaa
    14881 atataaggaa attagattta atatttgctc ttcatgaaaa tcagaaatat gatattataa
    14941 agacttttgg agtaagtgaa agtaaagtag ttgtaattgg tagtggttat aatgatgata
    15001 tattttataa taaaaattat aaaataaagg atgataaaat aaaaatagta tttgcaggta
    15061 agatatgtaa atcaaaggga ctgattccat ttataaaagc tatatctaaa ttaaaatata
    15121 gtaaagattt aatagaagtc aattttgcag gtactggaag cgatatcgaa tcatataatg
    15181 agatagttaa actggcaagt aagtctccat ttaaaatgaa tttcctagga aagttagaac
    15241 agagagattt agctgagcta ttcaacaggt cacaaatatt tgtgttacca tcattttatg
    15301 agggattacc agttgtagtt ttagaagctt tatcttgtgg cacagatgta atcactactg
    15361 atattcttgg tgttaaagag tggattggta gtgagattaa taattctgga aagatagaat
    15421 atgtttctct accttttatg gagaaagaag gtattccaaa agatgaagag ttatatgatt
    15481 ttgaaaataa tttgtataat gcaatagatt ctaagataca atctttattg aataactcaa
    15541 ataagaaaac atctgtagat atgtcaaaaa aaacgtggga tggcttagca tatagaataa
    15601 atgaagttat tttaatggat gaattgtgtt tagtctaaaa ttatttaaat tataaaaaag
    15661 tgtaactatt tttacactat ttttaaatga ttttatatta tactaatact aaatatattt
    15721 gttttaggga ggtaatttat gaggattagt aaaaaggtat tggcgttagg tatttcagca
    15781 ttactgttaa gtgttagttt tccaacatca ataaacgcac tagataagat tgaaaatatt
    15841 caaggagtag ataaatatga aacagctggg cttatttcag ataagcaaga ttttacaaca
    15901 gcagttctta tcaatgcaga ctctacaatg gtagatggaa ttgctgcaag tggtcttgca
    15961 ggagtaaata atgcagctat tttattaact aataaggatg atataccaga agcaacgctt
    16021 caaagactga atagagttac taagatatat gtaataggtg gagaaaattc tataagtaaa
    16081 gatgtagaaa aaatgttact tatgagaaga atgcaagtta taagaattga tggtgtagat
    16141 agagtagata caagttataa aattgcaggt gaaatagaaa aaattaaaaa cagtgataag
    16201 atgtttttag taaatggatt taaggatgaa gctgatgcag taagtgttgc atctgttgct
    16261 tatagagatg gagctcctat aatattgaca aaagatatac cttcagcaga agaagacaca
    16321 gacttagacc catggtttgg tgtttctcct gttccagtat atgctattgg aggagagtct
    16381 acattaagtg attacatagt gagcagatat agagcaacta gaattggcgg tattgataga
    16441 tatcaaacta ataaaaatgt tattgaaaag ttctataatg gagcaaaaga gttctatata
    16501 acaagtggag acgatttagt ttatgcttta gttgcatcac cacttgcaaa aaatgctcct
    16561 gttgttttag tttcaaataa aagtgataag agtatattaa gtggagctag taaagtaaca
    16621 gcaataggta taagtgataa gagtattata gagcaatgtt tggatgctgt aaagaagtaa
    16681 tggagtgtta ttagtaaaat gaatatatac attgaaaatt agtagattgt tcattttata
    16741 atggtataaa atatatggga agaggctatc aaatggagat aagcctcttt ttcattgtta
    16801 aaattatgaa aagatacttt attttatata aaaaagtatc ttatgaacta aatgtctatt
    16861 ttgatatact atctcctttt cttatattat attatttttt atatggaata aatttcataa
    16921 gtattatctt cctttaattt ttctaaccaa accatctcta aaatatctag ttcttctaaa
    16981 tcgtctttct ctgttttgtt atcatcatgt tttataagtt ctaaaacttc aaatgtaaat
    17041 gctttttcac catatttttt ccattcatta tttaattctt ttatcataca tgagccagca
    17101 caaagctgaa atttgagaga gttaaatttg ctttttatgt cattactttt tccaaggtaa
    17161 atctttttag taatatcatt ttttattatg aatacacctg tgtcaaattt catttctttg
    17221 tataattgtt ttaattcacg ttttctatcc atgattttag atacctctct ttataattga
    17281 tatgtaatat gtaagtgtaa aaatatatta catatcaatt ataaagaaat taattttata
    17341 tgtaaatagt ttttataaat ttaattatga attagtcatc aataaataag aaatttagct
    17401 gtagaaatcc attataaagg ctattataaa ttttaatatg ctaaaaatat aaaaagaata
    17461 gttgacagtg tagaaaatat ggaataaact aaaagtgtaa tagtaatatg aaattatata
    17521 ttacattgaa aggtgtgaat gattattatg aataaaaaaa tagaattaat agggaatttt
    17581 gcaagtgatt ggattaggta tagtgattat gaatataaaa ttactgaaga agatgaatta
    17641 tatatagttc caacagaaga tgctgtgttt accatgtata acccatttga tgtagcagat
    17701 gatataatag tagatataat tcgcatagga caagaggcat tacaagatat atcaaaagag
    17761 acaaaagaaa tgaaactaaa gaaagaagta cttgaatttg taaaaaagta tggattattg
    17821 ggtttaatat attcaagtgt atataataga gatatagttg gagaagaaag agtactgatg
    17881 attgaaaaga attatattac aaaagagaat atattgagta cagatgaata tgtaaacatg
    17941 tttataccat ttgcaagtga tgaagatgtg tattttaaaa agtatagaag gggtatagat
    18001 gtatcaaaga gagaagaatc tccaaagttt tttggaaaga gaccattgat tttagattta
    18061 gtattttcaa agttctacac tgaacaacta gattggattg taagttttgc taaagatatg
    18121 tgtaagcatt ttaatcaaat ttgtgtatat aaaaatacat ctaatctatt aactgaaagt
    18181 gttactatta tgtctggagg gtttaatcct caaaagatag gattttctat aagccaatta
    18241 gataaaccaa ttattgcatg ggagtttgat tctttgaaaa cagctattga gactatctat
    18301 gcctttgcaa ttacaagtga accttcttct ttaaataaat gcaagtactg tggtaaaatt
    18361 tatattccta caaattctaa atctcaatac tgttcacctt cttgtagaaa ttgctcaaat
    18421 gtaaagaaaa gtagaagtag agccaaaaaa gataaataaa attaataaat atatcattgt
    18481 ttagatttta gtaaataact actgaattaa aagttgttat agaaaattat atatctaaat
    18541 gatggtattt tttttgttga gaaaattgaa aagtttaatt gagatagtaa ttttgagttg
    18601 acaaaaacca cttttaacac tactattata agtggtttgc aaaataatgt atattaactt
    18661 aagtatagca taaaaaacaa aaaaagtcaa tggaaaaaat aaaaaaggag aatttaaaaa
    18721 tgatatcacc tattattttg agttctataa atcagaattt aaaggaaata gaaagaaatg
    18781 aactgcttga aactaatata gaatctggag attatggtct agctctttct gaatctgatg
    18841 taaaagatat aataaattcg agagataata ctttaaaggg gtatggaaga atagaattag
    18901 atataaaagt tacaaaacag ttaatagaaa atatatatac atcacaatat acaaatgtgg
    18961 acaattattt agaggctatt aatgatatgc aagaaatttt ttattattta aaaaatgaaa
    19021 ctgatgacaa gatatgtgat gatgagatta tagaaatact gggtgaattt tatgaaaaat
    19081 tttcaggcaa tatggacaat gtaagaggag aagcagacga atttgcaaag aaatttaaat
    19141 ttggagaagt ttaatatatg ataggagaga ttttgtatgt taaataattt aagtaatatt
    19201 aatataaacg aaaataattt aataaagaac caatattcca tatctctact taaggaatgt
    19261 ctaaattgta aagtgataga tgaaagagaa gtatataata tacaacaaga aatatctcta
    19321 atacttatgg atttgattaa aaaatataca aatggtcaaa gcacatcagt aaaaacagaa
    19381 gttgccgaaa aactgttgat ttcaatatgg tatgctattg atgcatatat aaataagctt
    19441 ggagatatag aaaaaagagt tgtggagttg actgctaaga atgttaaaaa agtgtacctt
    19501 ggaggaattg aaatattaga ggaagagtta gttaggctaa aagattttta taaactaatg
    19561 atgagtagaa aattggatac taattcaata gcatataatg ataccttgat ggagttatca
    19621 agtttcttta aaagttatat tgtaaaattt gaagctcagg acgttccagt aagtatagat
    19681 tatccattgg cactagatga tatggatgtt aagggtattt attatgtgaa gaattatatt
    19741 gagagtatag atatagaaaa tagattttgt aacctattta agaaaaaaga tagagaaaaa
    19801 ttattttatg actatgggat gacttataaa atagattgtg atgttatact tgtaaattta
    19861 tttgaactta ttataaataa ttgtattttt tctactattt taggaaatga agtgactaat
    19921 ttagaaattt caaaatatga gtatgaattt ttagaaaaac aatttaaaaa tatgtacaat
    19981 aatattactg aagataaagt tatagactct gaagaaagta atataattga attgattgaa
    20041 gtagatgata gttttaacac tgaagtagat agtaatttgg attataatga ttttgaagaa
    20101 attaacagag aaattagaat aaataaaaaa ataactttac tacttatgga agcaattgaa
    20161 gtattaataa aaagattaag tatagaagat gaagatgtaa taagatatat taaaaaatat
    20221 gaaaagatat ttattgaatc tgtgctaagc tcaataaaaa gtaacacttt taaaagtatg
    20281 gtaactgtga gtaaaaataa aaatgaacac ttagaagatt atattgttga tgatgaaagt
    20341 aaattggatg atgaatcgtt tagagagata ttcaatgaga tattagatag tacatctata
    20401 attgaaaaaa taagaataat aaaggaaaat atcaatgcta aaaaagactt tatcgatatt
    20461 cttgaatctg aatgtttatt tggagaagag tatttaatgt tgtttgcatc attaagtgaa
    20521 ttagaattgg caatattggg tagtgtagta ttttatgaag atattagaat gagagaaatt
    20581 aatatattag aatttatgtt aaatgagaaa actgaaacta ctttatggaa gatagagtac
    20641 attaaattta tgaagaggca aagtgaagat aagatatact ccatagaaga gcatatgaat
    20701 aaaattaatt aacaaaaaat tgtgaataaa tagaatatta gtatataata taatctgtaa
    20761 agcatatcaa aaatacatat atttggagga aaattatgaa agagtatatc ataaataatg
    20821 ttgaagaaat aagagaagaa ttaattgatt tatctaagaa aatatgggaa aatccagaat
    20881 tagcctttga agaaaagtat gcaagtagca ttcaaaaaga atacctaaag tcaaaaggat
    20941 ttaaaattga agaagtagaa aatttaccta ctggatttat agcaagtttt ggagagggta
    21001 aacctgtgat tggcatactt ggagaatatg atgctttacc agagctatca caatgtgtaa
    21061 gtgcagaaag aaagccattg gtagaaggaa aagctggaca tggatgtggg cataacctgt
    21121 tgggtgtagc aggtgtagga gcagttgttt ctataaaaaa attaatagaa gaaaataaat
    21181 ttaatggaac aataaaatat tttggttgtc ctgctgaaga agaaggtgga ggaaagacag
    21241 ttatgtgtat aaatggctgt tttgatgatg tagattgtgc atttacatgg catccttttg
    21301 atataaatgc accttggaga ggtggaagtc tggcaaactt atctgtaaaa tttaaattta
    21361 aagggataac agcccatgca gctcaagctc ctcataatgg tagaagcgca cttgatgctg
    21421 ttgaaattat gaatgttgga gctaattatt tgagagaaca tgttattgac tctataagaa
    21481 tgcactatgt tataactaat ggtggtggaa gacctaatgt agttcctgga tttgctgaaa
    21541 gttggtactt tattagaggt aaaaaagcaa aggatgcaga acacgttctt gatagattaa
    21601 taaaagtagc tcaaggagca gcaatgatga cagaaactga aatggagtat aaggttacag
    21661 atggaatata tgactatata ccaaaccaat gtttgactga tttagtttat gacaatatgg
    21721 tttttgttgg ttgccctaaa aatactcctg aagaagaaga atttgcaaaa aagttgtgtg
    21781 atactcttac tagagaagaa agattaggtg tagcaacatc attacaaaat gataaatcta
    21841 ttgtagaaag ctatatacac aaggaaatag ttgacggtga caaagataaa ggactagctg
    21901 gttctactga tgttggagat gtaagttatg taataccagt ggctcaattt gcaatggcag
    21961 cttggcctgt tggaattgca agtcatacat ggcagtcatg ttcatcagca ggttcaaata
    22021 taggattttc tgctatgata aattcagcta aagtactggc ttgtagtgct tatgatgtat
    22081 ttatggatac taaaattatt gatgaagcaa aaattgaatt tgataaatca ttagatggac
    22141 aaaaatttaa accattggta taattttggt aataaaatat ttctgggggg aatagctatg
    22201 agagaaaaag ttgagaaagt gcttgaggaa aagataaagc cagtattaca aagagatggt
    22261 ggagatgtag agcttataga tgtaaatgaa aatggtgtgg ttttagttag actacaaggg
    22321 gcatgtagtg ggtgtccagg agcgactatg acaatcaaag ctattattga aaatgttttg
    22381 gtgagtgaag ttccaggagt aactcaagta ttaggtgttt aatagttagc gtttattaaa
    22441 tgtatgttat acataagtat ttaataaaga agtaaaaaaa atagtgaagc aatatatatg
    22501 aaaaagtatg ataaagataa taaaacatta tagaaataat gaaatatttc atatgtatat
    22561 gaagaaataa gaaaaaatag tgaagcaata tatatatgaa aaagtatgat aaagataata
    22621 aaacatttat agaaataatg gaatatttca tatatacata tgaagaaata gtaaaacaat
    22681 atatatatga aaaagtgtga gaaaataata aaatactata gaaataatta aatattctat
    22741 ataggtatta agaaacatga aagaaataat ggagtattat aaataaatat gtaaaagtgt
    22801 gaaagaagga tgtagataaa tttgtcttat acaaatttac ctacagtatt tcttacacac
    22861 tttttatttt actatccagc cataccaata aacataccta taaaatatgg aactaaccat
    22921 atagtccata caactatact aaacttatga aagaattttt tcttttcctc atcatctttg
    22981 tataggacaa aagtagccca acttgcatga aaaagcataa gcacaattgc aagaagacct
    23041 gtaatactat gaaggttttg tgataaagca gattttactt caaaagtatg gctgttggct
    23101 atgttactca ttgtaaatgt tcctaaagta tcaaatataa aaccaagcca aaatataatt
    23161 acatgtttct tttttaaaat ttttgccttt ctttctccaa atacacctat agtgtagaaa
    23221 attaaagctg atgttataaa tactattgca agtattaatt tataattcat aattatctct
    23281 cctttacttt tgccaatata tttctaagat tttttactaa gtcactcaat tcgttttcat
    23341 cacaattttt aaaaatatca tcaaaacttt cttgcatttt aattttaaaa tttgtggcaa
    23401 attcaaatcc tgttgttgta aacttaacgt atgtatttct tttatcatta tatttcttga
    23461 atttttctat atatccaatc tgttctaatc tgctgattat gcctgaaacc gtacctttag
    23521 ctaaagacat ttcatcacat agttgtgata ttgtaagttc ttgattatga gctatcagtt
    23581 ttataactat aatctgttgg tgtgttaatc cattttcttt aaaattttct tctactgtgt
    23641 acatagtttt agaataaagt tcttttaaga gcatggctat tctaaaacag tcataattac
    23701 ttttcaataa aatcacctct taaatggttt gcatacaaac taatataata tacttttctt
    23761 atatagtcaa cctaaaattt tataatttta tagaaaataa tgaagaatag aatataaaaa
    23821 ttttatttta ggtataaata aaataatttg atgaaatttt aacaattttt aaaaagtttg
    23881 tttacacagt taataaatga tgctaaaatt aactcataga taaatataga aaatttaatt
    23941 ttgtttaatg ataatggaac cacgaaggtt ttaacgttac tttacgtttg cttttatgtg
    24001 gtttttttta ttgcaacaaa ggggggttgg ggtttgttaa aggatatatt gaaaaagttg
    24061 ttgcagttta ttttagtaat gtttttatta tcttttattg ttttttacat ggcaagactt
    24121 gctccaggag acccattaat ctcttactat ggggatggag tagaaagaat gagtacacaa
    24181 gaaaaagaaa atgctatgaa aaaacttggt ttggatgaac caatatacag tcaatatata
    24241 aaatggattt caaatgcatc aaaaggggaa tttggaattt catttaaata taaacaaaat
    24301 gttacttctg ttataaatga tgtctatata aacactataa tattaggtgg ttcaggatat
    24361 atacttacct ttgtgctagc cttattgtta ggaatatttt gtacattaca tgaagataag
    24421 ctcattgata gaatcatatg caaattagga acaataacca actgtattcc atcattttgg
    24481 gttgccttag tactcatact tatatttagt ataaacttaa gtatacttcc aagtagtggt
    24541 gcttattcaa tgggagaaga gtccagtata ttaagtagga tatcacatct aatcttacct
    24601 cttacagtac ttatattaag ccatctatgg tattacacat atatgataag aaataaatta
    24661 ctagaggaaa taagagaaga ttatgtgctt ttatgtaaag caaaaggttt aaataataga
    24721 actattgttt ttaagcattg cctgagaaat ataatgccat catatattag tataatggcc
    24781 atttcgatac ctcatatact aggaggaact tatgtagttg aaaaagtttt ttcttatcct
    24841 gggttaggaa cactttgttt tgaaagtgca aagtatcatg attataatat gttattagtt
    24901 ttatgcttaa taacaggggc tttagtagta tttgggaata tgttagctca aattataaac
    24961 aataaaatag accctagaat gaaatatgat agaggtgata ctaatgaagc cacaatatag
    25021 tgattttgaa attgtaggag aaaattatat tttagtagca gaagatgaac atatagaaac
    25081 aaagaaaacc ttatctaaaa aaattaaaga attaccatat atatcaatta ttattttatc
    25141 aatcatagtc attggaagtg tattctcatc ttttataatg acacatgaac caacatacat
    25201 ggatttagca agttctaatt tagctccaaa taaaactttt ttatttggaa cagactcaat
    25261 gggtagagat atatattcta tgatatggta tggaggaaaa atatctttat ttataggact
    25321 attttccact gtaatatcaa ctgttatagg aattgtttat ggtagtataa gtggttcagc
    25381 atctgaactt gtagatgatg caatgatgag gtttacagaa atcatactta gtataccttc
    25441 aatattaatt ataatatttg tacaagctat acttggaaat tcaaatccag tatctatgtc
    25501 catagtaata gggataacaa gttggatgaa tatatctaag atagtgagaa cagaagttag
    25561 acaaataaaa aatagcgaat acatactagc agctaagagc atgggaggag gattcttcta
    25621 tgtattaaag caacatttac ttccaaattt tgtggcatca ataatgttca tggttgtaac
    25681 aaacataggc gcagctattg gtactgaatc gacacttagc tttttaggaa ttggtttacc
    25741 aatagaaatt gtttcttggg gaagtatgtt atctttgtca gaggaagcat tactttcaaa
    25801 tagatggtgg attatactga ttccgggtat atttttggtt accacattgg tgtgtataac
    25861 aaatataggt aattatataa gaaagaataa taataaaaaa tctagtaatt tataaatcag
    25921 catttgagta ttaaataagc aaatactgag gtatttgaaa tatctattta aataaatggg
    25981 gtcaaagctt tgaaaaatat tttataaaaa gcaaaagggg gagcatcatg aaattaaaga
    26041 agttaaaagt tttaagttta gtaatgatac ttagtctaat ggcaggttgt tctagtggag
    26101 gagacaaaga taagaaggca gatacgccta aagatggaaa ggtacttgtt tatggaagta
    26161 atgactatac aagtataaat cctgcactat acgaacatgg agaaataaat tcgttaatat
    26221 ttaatggatt aacagctcat gatgaaaata ataaggtggt tccttgtcta gcaaaggact
    26281 ggaaatttga tgaggcaaca aatacatata cttttaattt aagagatgat gttaagtggc
    26341 atgatggaga aaaatttaca gcaaatgatg ttaagtttac aatagaaact ataatgaatc
    26401 cagataatgc ttctgagata gcatcaaatt atgaagatat aacaaaaatt gatgtggtta
    26461 atgataatac tataaaaatt actttaaaag ctccaaatac agcaatgctt gattatttaa
    26521 cagtaggagt attaccaaaa catgctttgg aaggtaagga tatagctact gatgaattta
    26581 atcaaaaacc aataggtact ggaccattta aacttgaaaa atgggacaaa ggtcaaagta
    26641 taacacttgt taagaatagt gattattttg taaaagaacc aggattagat aaagtagtct
    26701 ttaagattgt tccagatgat aaggctaagg caatgcaatt aaaatcagga gaattagacc
    26761 tagctcaaat aactcctaaa gatatgtcta actttgaaaa agacgaaaag aattttaaag
    26821 taaatataat gaaaacagcc gactatagag gtatattata caattttaat tctaaattct
    26881 ttaaagataa aaaagctaaa ggattaccaa atgctttaag ctatgcaata gatagaaaag
    26941 caatagttga cagtgtatta ttaggacatg gtgtaccagc atattcacct ttacaaatgg
    27001 gaccatataa taatcctgat atagaaaagt ttgaatataa tccagaaaaa gctaaacaag
    27061 aaattgaaaa gctaggttgg aaattaggtt ctgatggaat ctatgaaaaa gaaggtacta
    27121 agttagcttt cgagataaca gcaggtgaga gtgaccaagt tagagttgat atggctaaaa
    27181 tatgtgcaca acagttaaaa gaaattggtg ttgatgctaa agctgttgtt gtaactgaaa
    27241 cagattgggc taatcaagat gctcatttaa taggttgggg aagtccattt gacccagatg
    27301 accatacata taaagtattt ggaacagata aaggtgcaaa ttacagtgct tactctaatc
    27361 ctactataga taaaatactt caaaaagcaa gagaaacaga agataaagat gaaaaattaa
    27421 agttatataa acaattccaa gtagaaatga ctaaggatat gccttataca tttattgctt
    27481 atatagatgc aatatatgtt ggaaaaccaa acataaaagg attaacacct gatacagttt
    27541 taggtcatca tggtgtaggt atattctgga acatagctga ctggacaatt gaataatact
    27601 gaaattaatt agaaacctta gggataaaaa tatatcccta aggtttgaat agcatagggg
    27661 ttgatggttt ttggaacatt tattggaggt taataatcta tctgtaagtt ttaaagtaga
    27721 agaaggagaa gttcaggctg taagaaatgt atcttttaat ctaaaaaaag gtgagacatt
    27781 ggctatagtt ggagaatctg gttgtgggaa atctgtactg tgtaagagtt tgatgaggat
    27841 actcccatac aatggatata ttaaaaatgg agaggtttta cttaaatcaa gtgatttggt
    27901 taagaagtct gaaaaagaga tggaagatat aagaggaaag aatatatcaa tgatatttca
    27961 agaccctatg acatctctca atccaactat atcaatagga aagcaaattg cagaagctgt
    28021 aatcattcat caaggtataa gtaaaagtga agcaaaaaag agagccatag agcttattga
    28081 attggttgga attgataatc ctgagaaaag atttaaacaa tttccacatc atttttcagg
    28141 tggaatgaga caacgtatag taatagctat agcacttgcc tgtaatccag atgtattgat
    28201 tgctgatgaa cctacaactg ctttagatgt aactatacaa gctcaaataa ttgatttaat
    28261 aaaggattta caacataaaa taggtttgtc aataatattt ataactcatg atttaggagt
    28321 agttgcaact atagctgata gaatagcagt tatgtatgca ggaaagattg tagaaatagg
    28381 aactgtagag gatatatttt atgaccctag acatccatat acttgggggt tgttgggttc
    28441 attacctact ttagattcgc aggatgacta tttatataat atacctggaa tgcctccaaa
    28501 tttattgaac ccaccaaaag gagatgcttt tgcaataagg aataaaaatg cacttaagat
    28561 agattatgaa aaagagccac caatgtttaa aattaatgat acacatagtg cagcaacttg
    28621 gttattgcac ccagatgcac ctgaagtaga tgtaccagtt agagtaaatt gtggaagggt
    28681 gatttctaat gaatagagaa aaaatactgg aaataaaaaa cttaaagcaa tattttcatt
    28741 tggacaaaag tactactgta aaagcagttg atgatataag ttttgatata tataagggtg
    28801 agatatttgg attggtagga gagtcgggtt caggaaaatc tacaactggt aaaactataa
    28861 taagactaca tgaatctact ggtggagaag taatttacaa aggaaattgc atatcagata
    28921 aaaaaacgta caaatttata aaaaaagatg taaacaaaag tatgcagatt atttttcaag
    28981 attctacttc atcattaaat ccacgtatga caattgcaga tataatttca gagccactaa
    29041 aaattcaagg tatctgtaaa aataaaacag atagaatgaa taaagtatat gagatgctaa
    29101 agctagtagg acttgataga agttatgcaa ataagtatcc atctgatttt tcaggtggac
    29161 aaagacagcg tattggcata gcacgagctc tttcagtaga cccagaattt ataatagctg
    29221 atgagcctat agcttctttg gatgtgtcta tacaagcaca aatagtaaat ttatttaaaa
    29281 aattgcaaca agaaaagaat ttaacttgtt tatttatagc tcatgattta tctatggtga
    29341 gacatattag tgaccgtata ggagttatgt ataatggaaa attagtagaa ttggcagatt
    29401 caaatgaatt gtataataat cctattcatc catatacaaa atcactactt tctgcaattc
    29461 ctgttcctga ccctagatat gctaagtcaa gaaatagaat agagtataat tctaatggat
    29521 atgattgttc aaatgaaaag ttattaagtt ggattgaggt ttcagatgga cattttgtat
    29581 attcttctaa atcagacata aataaatacc aacaaaattt aaaagttgtt tagtataaaa
    29641 atacttgaac aggtatttat ttgaaataca aactaaaacc ctatttttgg agaaatttta
    29701 aaactactcc aaaatagggt tttttatttc tacttttcaa atataataaa ttcaaatata
    29761 ataaaattaa aaacaagtaa agtatgttgt taagaaagta ctataaaatt aaaaatagta
    29821 atgtagaaaa aatgagcaat aatcttattt tttctacaat aattttctat aatttaaagt
    29881 aaggaaagtt tgataaatta taaccttagt taaagaataa gagtttatta aaaaataaag
    29941 tgtgtatagg ggttttagtt aataaaatac tataatgtag gggagcttgt aatgatatat
    30001 aaaaaagata aagtaatgaa agttttagaa tcatcatata gggaaaatga aaaagggatt
    30061 acagctaacc aattatcaga aaagacagga attgcaagaa acaatataag tacatattta
    30121 aaccaattat tcaatgaggg tatggttacg aagataaaag gaagacctgt gtattatatc
    30181 cctgcaaatc ttaaccttaa caagaatgaa ttgcttgata cacaaagtga gttgcttggc
    30241 ataaaaaaag gaacaaaagt agaaaacaaa ataaaaaaag atagttttga aacactaata
    30301 ggtaaagaac atagtttaag acctataata gaaaaatgca aggtcgcaat gctctatcct
    30361 acaaatggac ttcatactat tatctatgga gaaacaggag taggaaagag tatgattgct
    30421 agatatatgt acgattattc tatagatagt ggaattagaa aaaataaagc tccttttgta
    30481 acatttaatt gtgctgatta tgctaataat gcacaattac ttatgggaca tatttttggg
    30541 gtagaacaag gagcttatac aggtgcagaa tcttccagaa aaggtctttt ggaaattgca
    30601 aatggaggga tactattttt agatgaaatt catagactac cagcagaagg tcaagaaatg
    30661 ttgtttactt ttatagataa gggaacattt aaaagactag gagatgcaac gaaagaaaga
    30721 gagtctaagg tattaataat atgtgctact acagaaaatc cgacatcaac actattagat
    30781 acattcaata gaagaatacc aatgaaaata gaaataccat cattgagaga taggacaatg
    30841 attgaaagaa tggaattagt caaaaatttt ttcaaggaag aatcaaaaag tatttctatt
    30901 tcaataaaag tacataaaga aataattaaa tcactattac tttatgattg taaaaataat
    30961 gtaggtcaac ttcaaaatga tataaaatta gctgttgcaa atggatacct aagatataaa
    31021 agagataata aagaaccagt ttatatagag aaaaaatatt ttgataaatc tataaatgaa
    31081 gtgagtgatg attatagaga gaagtctatt aaagcagatg aagttataac acatggaata
    31141 gaatattttg tatttacgtc attaggagaa gaagaagtta ttaactacaa tgatataaat
    31201 attattaatg cagtaaatac taatcttaat aaatctgtag aaatgaatat agcagatgct
    31261 tctctattta ataatgttaa gtttaaaagc atgtgtgaat caataagaca aattattaaa
    31321 gaagatgaag aaatagatat aagtgaatca atatttaagt caatggcaat ttatataaaa
    31381 agtgtattgg ataaaagtag ctctacaaac aacatagatt tgaatcaaat tagaagaaat
    31441 aacagaagtg aatttaaaac agcattaaaa atagttggaa taattgaaaa tgaatttgat
    31501 ttatttttat ctatagaaac tgtagcatat attacattat tcatagtaca agctaaacaa
    31561 gatgaacaaa ataaaatagt gaataacatt aatattatag ttgctatgca tggagaaact
    31621 acagcaagta gtatggtaaa agcagttggt gatattattg gaaagtgcag tgctatttct
    31681 ttcgatatga agttagataa aagctatgat gaagtaattg tagattttaa aaatttaatt
    31741 aataatatag acaataaaga tgtattacta tttacagata tgggttcatt aaactctttt
    31801 gatgaaatta taaagaaaga aaaaaagtgt ggagtaagag taattcctat ggtgacgaca
    31861 ttaactgtgt tagaagcagt tcagaaggcc aatatgggat tacctttaaa tgacgtatac
    31921 aactctatca ctaacacgag aaagtactat tttggaacta atgaaattca aaataaagaa
    31981 aatctgtcta aaacaattat tatagcatca catgtttcag aaggtgtaga taataaaaca
    32041 agaaaaatat tagaagaaaa gatgagtagg tacttagatg gaatagatat tatatctgtc
    32101 ccatataaaa cagaaaaaga tttaagttta aatattacga aacttaaaga aagtagtaat
    32161 attgttgcag taatcaatga acaaagaatt aatattagag gaattgacta tattagcaaa
    32221 aaagatatag ataaggatga aaatatcaat aaattaaaaa atattataaa gataagtata
    32281 ggttatgatg atgtagttga aggtttaaag acatccttaa aaagctctaa ttataatagg
    32341 atatttaaag atataaaata tgtgtctgat gaattgtttt tagtatttaa tatagaaaag
    32401 aaatacgata aagtaattgg acttatgatg catttagctt tcatggtaga tgggttgatt
    32461 ggaaatacta gagaaattga aaagcttgat aaagaaaaaa cacttgatta tcacaagagc
    32521 ctaagcaaaa taaaagatat agtatctcaa ttagataaaa aatataatat agaaattaat
    32581 gaaaaagagt gctatcaaat attattaata ttagaatatg cagaaataat cgaaaaagat
    32641 taccaatgag tataaaaact tatttggtaa ttttttttag tctaaaataa tattaatatt
    32701 agacacatat aaatatactt aaacaaagta tctaagtttt gatgataaaa atgattgaaa
    32761 taacagtgtt ttaaagtgtc taaaaaaact ggcataatac ttgcttatat tattaaatga
    32821 gtaatataaa taattaaaat ttaaataaaa aggagtaaat aatatgcaag ataaaatatt
    32881 aagagataaa aaggcaaatg acccatgggc aagaatgact acaagaaacg gatttagtgc
    32941 agacgaaata atatcgtcat ttcaaaaagc tataagaaga aatatggtgg aagaagcttg
    33001 tgaatttgct tatgaactat atatatcatc accagaatta gaagataagc tatggagaag
    33061 gatacttaca atatcagttg aagatatagg aatgggggac ccatcagctg taataatcat
    33121 aaataattta aatcaagtaa gaaaggaata ttcatatgca gatggagaca gacctttatt
    33181 tttcatacat gcaataagat atctttgtgc atgtgagaaa gatagatcaa gtgatttatt
    33241 aaaaaatata attataaaag gatttgcaat gggtaaagtt ccagaagtta tggacgtggc
    33301 acttgacaag catacatata gaggattaga aatgggaagg gattcattcc actttttaaa
    33361 tgaagctagt atagtaatac cagaaaaaga agtagataat gattataaag aaagatactt
    33421 aaaaatatta gaaaaatata agcaagaaga agttatagat acggctttca agtttaatca
    33481 ttggcagtac taagaattag agtaaatatt actaagaatt aaaataaaat aataagaatt
    33541 aaagtaaaaa ctgataggaa ttagagtaag aattggctga taaaaaatta gaaaacaaaa
    33601 gaggttagat attgtaggag ggtaattatg aatattttat tagtatgtgc aaatggagct
    33661 tcaacaggtg tacttgtgga aaagatgaaa agtttttgta gtgagcatga aaagttaaaa
    33721 actaaaacta taaatataga ggcaacatca tttgaaaatt taaaatcata cattgaagct
    33781 aatgatacag atgtagtact tgtagcacca caaattaggt ttaaggaaga tgatgtagtt
    33841 gaagcttgta agaattacaa aatagcagtt ggtttaatag atactaagca ttatggtcgt
    33901 atggatgctc cttctgttat gaagtcggca atcgaattat ataaaaatag ataattgggg
    33961 ggaataaaaa tggaagcact tcaaaataat ttaagaagat ttcttcttcc aatagcacaa
    34021 aagattgaaa aacaaaggca tcttcaagct ataaaagaag gtatgataag tattacccca
    34081 ataataattg ttggttcttt aagtatgtta tttatggcat taaacaatat gttacctgaa
    34141 ggaagtgcaa aaacattatt atctgaaaat atggatacat tacttatccc taataaattt
    34201 actatgagtt tattatctat atattcagca ttttttatag ctcaggcact agctaaaaaa
    34261 tataatctta atcatgtaga aataggtatg acagcagttg ttgcacaact tgttgtatgt
    34321 ggacaagtag tagatggtgt attagataca agttatttag atgcacaagg tttatttgta
    34381 agtatattag tagcactttt agtagttgat attactaagt ttatgaatga taaaaatctc
    34441 gtaattagat ttccaaaaga agtacctagt gtagtaaata aaagttttag aaacttaact
    34501 ccaatgatag tatgtataat gttatttaca gcaatagcag caataactaa aaatgtctca
    34561 ggtcaaccac taccagctat tataatgaat ttcttagcat cagctataag cagtgtagat
    34621 aatgtttttg cagtaactat aatactattt ataactcaat tattatggtt ctttgggctt
    34681 catggagcag caataacttc aagtatatgg atgccaatag cagctacata tatggcagaa
    34741 aatgcaacac tgatagcagc aggaggagac ccaaaatatg tgtttacaat aggattttac
    34801 tatggtttct tgcaagtaac tggttcaggg ataactttgg gattagtgta ccttatgtca
    34861 agaagtaagt gtaaaagttt taatagtatg ggaaaagttg tgattttacc atcactattt
    34921 ggaataaatg agccagtaat atttggaaca ccaatagtta tgaacccata tatgtttgta
    34981 ccatttgtat ttggacctgt attggttgga gcacttaatt ttatggcatt aaaagtagga
    35041 ttagtaggat tacctattgc agaaccacca ggttttttac ctccaggggt tgcagcattt
    35101 ttgatgacat tagattggaa ggcaatcgta ttagtatttg caagtataat acttatgacc
    35161 ttgatatatt atccattctt taagataatg gagaaggaag aattaaataa aaatgcagaa
    35221 ttagctacaa cattagatga tgatagcttt gatttttaac aagtcattag aaggagaagg
    35281 atatggtacc tgaaatggaa gaaaaagtat ttagtataat atcagaagca ggagatgcta
    35341 aaagtgatgt aatgatgtct cttaaggaaa taaaaaaagg tgactacaat aaagcaaaac
    35401 accttttaaa ttcagcctca gagaaaatac aaacagcatc aaagtatcat ttagaattac
    35461 tatcaaatgc tatgaatagt gaagatagtg ggactgattt tttagttgtg cactctgaag
    35521 accattattc aaatgcatta tttgctcatt ctttagtatc tgaacttgta gatatatttg
    35581 agatgatgga cccaagaatt aataagaatt aaatttcttg taaatagtgc ttgaagtttg
    35641 gctatagttt attaaattaa tagatttaat aaatgaactc ccttttactt taaagtacta
    35701 gtatttataa atatatttat aaatacagta aatttttaaa tagaaaagga gatattatta
    35761 tggaaaaatt ttcagtagta atagctggag gaggaagtac atatacccca gaaatcatat
    35821 taatgttgct agataactta gataggttac cattaagagc aatcaaatta tatgataatg
    35881 atgaagaaag acaaaataag gtagctaaag cttgtgaaat actaataaag gaaaaggacc
    35941 ctaatataga gtatttagca acaacatgcc ctaaagaggc ttatacagat gtagattttt
    36001 gtttagctca tataagagtt ggaaaacttg aaatgagaga actagatgaa aagatacctc
    36061 taaaacatgg ggtagttggt caagaaactt gtggaccagg tggaatagct tatggaatga
    36121 ggtctataga aggtgttctt gaaaacatag attatatgga aaagtattct ccaaattgtt
    36181 ggatgttaaa ttattctaat ccagcatcaa taatagcgga agctgttaga aggttgagac
    36241 ctaattctag ggtaataaat atttgtgata tgccaatagg tatggaacat aatatagcaa
    36301 ggattgcagg actcaaatct cgtaaacata tggatataag atactttgga ttaaatcact
    36361 ttggttggta tacttcaatt aaagataaaa aaggaaatga acttttacca aaattagtag
    36421 aacatataaa agaatatggt tttataaatg gtgaagaagg aatgaaaaat gacaagaaag
    36481 atagttggtt tgaaactaat ttatttacta aagaaatagt aaaaacagat ttaactacaa
    36541 taccaagtag ttacttaaag tactatctat ttccagatta tgttgtaaat cattctgatg
    36601 taaattatac tcgtgccaat gaagttattg atggaagaga aaaagaagtt tttggttcat
    36661 gtgcattgat agaaaagcaa ggacattcta agggcagtgg gcttaagata ggaatacatg
    36721 cagagtttat agtggattta gctacagctc tagcttttaa tacacaggaa cgtatgttat
    36781 tgatagttca aaataatgga gctatagaaa atatacaaga tgatgctatg gttgaaattc
    36841 catgtatagt tggaaaagac gggtatgaac cattatcaat agggaaaata ccaacattcc
    36901 aaaaaggttt aatagaacaa caattagcag ttgaaaaatt agtagttgat gcttggattg
    36961 agaagtctta taataaattg tggcaagcac ttatgctatc taagacagtt cctagtgcaa
    37021 cagttgcaaa agaattacta gatgatttaa tagaagcaaa taaaaattat tggccaaaac
    37081 ttaattaaga ataatatatt tttaaagttc ctacagttta gtacatagcc tagttaatgt
    37141 atttgtaggg actttattta tttacaaatg agatacaagt ggcaaacata gtgtattttg
    37201 cagttaggaa agtaggttta gttacttgtt agttggatta aatttaggta caataattta
    37261 ctatcaatca atagaaataa ccagagaagt tgcataataa aaaatttaca cttttaacag
    37321 gatttatgta ggtgtttata gaaataaata aatatgtaca taaatacata attattttta
    37381 agtctaaaat acaagaatgg agagatggct atgaagtgga gagattatgc tgatgatgta
    37441 aatttatttg aggattttga taaaactatc aagcctctaa ctgacgaaca aagaagaaaa
    37501 aatataaaca ctttaattgc atatttttca aaagaagttc cttctaaaat atacaattta
    37561 tcaaatgatg aaattaagcc tagagatata cttagaggac ttttgaatgt atatccacca
    37621 aaagaaattg ctccagagat acttaatatg ttacataatt tattgttgat tgaatgtgaa
    37681 gaaagagagt tagtagatgt aaatgatata gaagaagtag aggaagggat tgccatatgg
    37741 agaagtaata taacgaattt aagagcagat gcaatagtaa atgctgcaaa taacaagtta
    37801 ttagggtgtt tacaaccatt acatctatgc gtggataatg agatacattc atgcgcaggt
    37861 cctagactta gagaagattg tgacaaaata ataaaaaaac aagggcattt agaatatact
    37921 ggagatgcta agataactag aggttattgt ttacctgcaa aatttgtagt acatactgtt
    37981 ggacctattg tatctggtgg tcagccaagt aaagagcaag aaaagcaact gcttcattgt
    38041 tataaatcat gtttaaatac aataaaagaa attgatgaaa taaaaaatat tgttttttgt
    38101 ggaatttcta caggtgtatt tggttatcca aaaaaagagg cagctaatct tgctgtaagt
    38161 agagttagac tatggctaaa agaaaatcct gaaaaaaatt taaaagttgt atttaatgtg
    38221 tttactgaag aagaagaaga gaaatataga agaatattta aataatatta tgtaacagaa
    38281 tcctaaaaat acttattata taatacaaat tcattgtata agataataat ttacaagtag
    38341 taaaaattaa taaaatataa tttgtattt
//
LOCUS       DAEMIK010000020        37000 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEMIK010000020 DAEMIK010000000
VERSION     DAEMIK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.020"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1019
                     /locus_tag="KPK87_000868"
     CDS             <1..1019
                     /locus_tag="KPK87_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2537043.1"
                     /translation="EKLYYTALIGYIFYEAPREEKNFATLLDMIDASEVREDDETYMN
                     PIDRLFEALEKKEPTHFAVKQYKKYKLAAGKTAKSILISCGARLAPFDIRELRDLMGE
                     DELELDTLGDRKTALFVIISDTDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVR
                     CLLDEFANIGLIPKFEKLIATIRSREISASIILQAQSQLKAIYKDNADTIVGNCDSTL
                     FLGGKEKTTLKELSETLGKETIDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMD
                     GGKCIFQLRGVRPFLSDKYDITKHKNYKLLEDYDKRNLFDIESYIKRKGKVKMNRHTV
                     ITRL"
     gene            1183..2049
                     /locus_tag="KPK87_000869"
     CDS             1183..2049
                     /locus_tag="KPK87_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2537044.1"
                     /translation="MNIIKSFNSTIDYLETVLDDEIDEKKVTRISGYSYSMFSRLFSI
                     LTEMTLSEYLRSRRLTEAAIILRDTDEKIIDVAFKFGYESSDSFGTAFKNFHGFTPSE
                     VRIGKPFKLVSRVQLALSVRGGRSMNIRIQKKKAFTVAGVNQQNINSSICASLWQKLF
                     SKVSHEEMLNLGTGEVVGVCHDILSLDNHDVLDSNTINYMAGYIVTDVDKARSMGLDI
                     LEIGETEYAVVELTGSVPECIHNGWKYAMEVFFPEHGYVHSGKPDFEYYYEGDMYSKD
                     YKMELWIPVCKS"
     gene            2258..2569
                     /locus_tag="KPK87_000870"
     CDS             2258..2569
                     /locus_tag="KPK87_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-strand DNA-binding protein"
                     /protein_id="HBG2537045.1"
                     /translation="MKQEMININANLLAEPTFSKFEKEGEKVEVANFTLVKKYGKGKE
                     YINCAAYGEKAKTVKAFEKGDLIHIFGYFKKREKDGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            2573..2788
                     /locus_tag="KPK87_000871"
     CDS             2573..2788
                     /locus_tag="KPK87_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG2537046.1"
                     /translation="MEFFTQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLR"
     gene            2808..3671
                     /locus_tag="KPK87_000872"
     CDS             2808..3671
                     /locus_tag="KPK87_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537047.1"
                     /translation="MFGIFDKIEEFFKEFLLGGIQANLESMFLDINDKVGAIATDVGK
                     TPMGWNGQVFSFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFDFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSATVSGNQIVAMIDSLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYIYSSVSAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIKITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            3689..3949
                     /locus_tag="KPK87_000873"
     CDS             3689..3949
                     /locus_tag="KPK87_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537048.1"
                     /translation="MNKRKTVFIVVIGAGIMAVIDRIRLHSKVNDLEERKKDIGRCHN
                     DFCLMQERFNRSIDEQITSIQEEIGSVYEHIEELSKGKEDGR"
     gene            3953..4342
                     /locus_tag="KPK87_000874"
     CDS             3953..4342
                     /locus_tag="KPK87_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG2537049.1"
                     /translation="MAYVPIPKDLKKLKTKVAFNLTKRQLIGFTIAGLIGIPIYLFMR
                     KVVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QKKKDSAAKVRTKQKEVKKSKTGLKAK"
     gene            4254..6683
                     /locus_tag="KPK87_000875"
     CDS             4254..6683
                     /locus_tag="KPK87_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2537050.1"
                     /translation="MNKKRKIQQLKLEQNKKKLRSQRQDLKQNKGKSKKDKGGLLDPI
                     FRKEPKRYTVEDTIPYLRLLKSGICQIDENHFNKSIAFEDINYQLALEEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKGDGFDDIRLEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERMEIDILSSFKSMGVRAESLNGEERLKILHD
                     VLNPNKTFEFSYKDLKKKESTKTYIAPDEFNFTPSRYFKFGNFIGAASHFQILASELS
                     DRMLSEFLDIDDNINISFHIKAIEQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVTIIFMNFARTIQKLDNTISQISSIANKH
                     NCKLKRLDHSQEQGLISVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSANSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILTTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYGKYFENPILENMPILQDLYDMLKNQEEKVGKKLATEMEIY
                     VTGSLNVFNNRSNVDLNKQLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTTSYSVEIWKRFRKWGGIPTGITQNVKDLLMSNEIENIFDNTDF
                     VLMLNQASGDREILARKLKISQPQLKYVTNSNAGEGLLFFGNTIVPFLDKFPKDTILY
                     QKMTTKPEEVR"
     gene            6688..8850
                     /locus_tag="KPK87_000876"
                     /pseudo
     CDS             6688..8850
                     /locus_tag="KPK87_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8862..9095
                     /locus_tag="KPK87_000877"
     CDS             8862..9095
                     /locus_tag="KPK87_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537051.1"
                     /translation="MKKELTAVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLMAKVNREKSEMKKENGNEKNEE"
     gene            9076..10650
                     /locus_tag="KPK87_000878"
     CDS             9076..10650
                     /locus_tag="KPK87_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG2537052.1"
                     /translation="MKKMKNKKAAGVILGAVLLIVGVVISLFAGSKIVFAQGGLFTTL
                     VNPIEAVAQIEVEVNVKYIFEDEKVFKEEKIKAEKEWLLDSGDLPIIPDDMKFIDEFL
                     FYEVKGDGKDEIIRKVTKIDVKDKETQTENESSKSEQSSQSNASKIENKGTQTELSKD
                     DISKMERESKELKEKLDKLNQEIKDKDKLSEKQKDKIKDLEEKIDKLKEKLEKGKDDK
                     DLSADMKKEIDKLTDKIKELEKKANEVGRAPIMHNPNVTPISPISGIKQGTGNVSPQT
                     SINSSGKGSATQESKGANTKDTNSNESKEKEKQVRYPNKLTPKQPANNGSHDSSLDGS
                     SKNINTNKGVASEPSKARGTVTENKDNANKNYPIHHNDSKDNKETDKYSADARQFVTF
                     TTKNGKTFHLIINHDEDSENVMLLTEVSEDDLLNMVEKKAPEKEIVKEESQKEEPKQV
                     KKEESSNTGTYLILILVVAGALGAGYYFKVIKKKEDKELEGFEEEDDDFFSEAEESEN
                     EVNEAVTEDKEDDEIE"
     gene            10746..12452
                     /locus_tag="KPK87_000879"
     CDS             10746..12452
                     /locus_tag="KPK87_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG2537053.1"
                     /translation="MKLVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGHLI
                     QMANPESYDEKYAKWNMADLPIIPREYKYEIAKSTKKQFTILKKLMNDKEIDIVINAC
                     DAGREGEAIFRLVYNQANCKKKMKRLWISSMEDSAIKEGFDNLKDGSFYDNLFESAQA
                     RAIADWLVGMNLSRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYFTVELD
                     LDGFSLSTDRIDDKTVTEQLINLLGNSIEITDVIQKEKITKPELPFDLTTLQRECNKY
                     LNYSAKQTLDYTQSLYEKKLITYPRTDSRYLTEDMITSTINNIIGKHDFDTGRIKMVF
                     NSKRVTDHHAIIPTASSMNEDLASLPESELKVYELVLNKLHASVGYPLIENTTKIVAV
                     FDGFEFTSSGKVIVDEGFTKYLKKYIKKKNEDTVFPDLKIGDSLDIKEKEIKEKYTSP
                     AKHFTEDTLLKDMELAGNDALEKGVEVERKGLGTPATRAGIIENLIFKGFVERDKKNL
                     IATHKGISLVTIVSDTFKSAGTTAKWEMELSDIAQGKASKENFLKAIENEIKELVSTY
                     SK"
     gene            12536..12721
                     /locus_tag="KPK87_000880"
     CDS             12536..12721
                     /locus_tag="KPK87_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG2537054.1"
                     /translation="MSEVFMNNAKVMAKGQVTIPKKIREILEIENGDYVTFVVTEGKI
                     QIVNSKTFIEKNIQGRK"
     gene            12755..13801
                     /locus_tag="KPK87_000881"
     CDS             12755..13801
                     /locus_tag="KPK87_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBG2537055.1"
                     /translation="MNKEKNSLQNINIEKNNNSFEEIVMIVENAKDRAYRKVNEELIL
                     MYQEVGKYISEKTKEASYGSGFVDNVAEFFSINYPDLKGFNRRGLYRMKQFYELYKDD
                     EKVSTLLTQLSWSNHLKIMSGAKSREEREFYINLAIKENLTHRELVRQMDSGYYERCM
                     LSNEGNMPAIQRAKQETHNLFMDSYVLEFLDAPKIGNEREFQKSILENLKNFVLEIGK
                     DFSFIGNEYRVQVGNHDYYIDLLFYHRGLSCLVAFELKIGEFKPEYVGKMNLYLEALD
                     REVKKQAENPSVGVILCASKDDEVVEFALSRSLSPTMVSEYRLKLIDKELLQRKLKEY
                     TGIAEQEIERHDEK"
     gene            13912..14718
                     /locus_tag="KPK87_000882"
     CDS             13912..14718
                     /locus_tag="KPK87_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2537056.1"
                     /translation="MRTGNEFNGVEDTLYIPLAARIYVSEKFPHFFYDEKALSLKPKI
                     PTGGIDNNSTEYFYMASVCRQQTIDMKIKKFLDENKKGNMVFLGAGLETSYNRIGNQI
                     VNFYQVDLPGVIEVRKRVLGNAKNEKLISGDMFDLNWIKEIDTSLPTLISVAGVYQYF
                     DEYKIIDMIQKLKAQFSKGELIFDATNSKGLKLANKYVKKTGNKNAQMYFSVDNSKDL
                     ADKTGTILLEVDGFFHGALKACQGLKLMTRLYMYFADKLHRTLVIHLRFN"
     gene            14831..23812
                     /locus_tag="KPK87_000883"
     CDS             14831..23812
                     /locus_tag="KPK87_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG2537057.1"
                     /translation="MRINDFHNILELVKQDILQSEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHKDVLKEMIKTEGYEESESTLENIFSLSRLYGDEKIDNLMNELR
                     IADEDRISFTKFVRDSISYAVASRFKLDYPMDKELLKENFAMLDSISLMSLGETVSDI
                     SGTVIDTTIQKSKELELKKEVLRGKEAGYNKIKEELEEVEENVLRRDDQERISENERI
                     FRNGKYGRDNRENQGEYTESVRGRDGLYEGISESSVRSDEAELSFKQRRAEPLRDVSG
                     SIQGEEADQTPDGYSETGDRVYESRKAKTDDVLADREREPSSVQSNDFSPQRNDNQGS
                     SGNLKENTDKEIREADKASFSLPENSSGQIKLTIPLAQNDIDSILVNGGNHEGGRLPI
                     IAEFSKGKTVEKLGEYLKDTMGGGNGFFLNGTEVSSWYSDKGIHLAYGTSAREDMTQI
                     LSWSDAARRINELLENGEFATNVELLEAQDYERNRISESLWYLYHDLSEEGKAQGYFD
                     FIETGGGFPKETRQLSEALKNPDYLKDVVSEYSRFLTGYKENKEVLRFHYHKVDSLYQ
                     RLQELALTRKEYTSNLIELPKIQGFITEDEVLATISRGSGVDKGKERITKFFKENHTL
                     QEKANFLKGEYGIGGGSHAVSGERGSGEWHDAKGLKLQKNHCNDVFLTWSNVAKHIDE
                     LLSKNLYLEEKNIEKKVEIEEQKEPSYYSKDDPYNLITDEMLEKVPELYAQEDVALAD
                     KQVHAAYIIPFRSNWTWYMTEYDRESKDAFGLVLGIEPEWGYFNLDELKELNAQRLVL
                     EDFPKTFRELKDSELKKQMDEQELYQVFNGELSFEENNIELEAPEDVEESKQAEPFQA
                     TLFDYLKEKEEVELNEKENSLSDEFTVKEGDTVYFNHEEYKVRDLSKNQITGKNDLWL
                     DPVRQGNHQIPIVAFTDNEDLLRQISLERPEFIIGDEVKYKGKDYAITKFDNMGNNLK
                     TVTVKDNTEYLGGMITGSDVIPYRQESELNKIFENLTYKKEELYDLAVRIDEIAYDFD
                     SYDYADSVEAESEKRLDNINQIYGDLQAGNVSVYINSLNEMIEEDVEDTEIIKQVLTQ
                     LEEKKIDIQPKQEVFNFKITEETLPQRLSPSERLNNNLEAISMLNRVESGERELDITA
                     QEVLAKYVGWGGLSDVFDESKDGQWKDARAFLKENLSSSEYEAARESTLTSFYTPKIV
                     IDGIYKTLSGMGFKQGNILEPSMGIGNFIGNVPDEMGKSKFYGVELDSVSGRIGKLLY
                     PESEVQVKGLEETGFSNNFFDVAIGNVPFGEYKVNDREYNRNNFLIHDYFFAKSIDKV
                     RNGGVITFITSSGTMDKKDESVRRYLAARAEFLGAIRLPNDTFKGVAGTEVTSDIIFL
                     KKRDSVLERDEAWIHLSEDKNGLVYNKYFVDHPEQVLGSMREVSGRFGKTLTCEPIAY
                     LGQEINMASLKERIEIAGERISKDTKYEEIELLDDEITSIPATDDVKNFSYTLNDDEV
                     YYRENSLFIKKEVSDKNKEKIKDYLELNVALKDVIYKQKEDFSEEEIKASQEKLNEVY
                     DNFSKKHGFVNNLSNTRALKEDSNFPLVSSIEILDEEENFKAKGDIFSKRTITKAKVI
                     DHVDTSLEALVLSVSEKGYVDFDYMGSLTGKDRATLIEELRGEIYLNIREEQNFYRSL
                     SFNPEDGDLPFACANGSNSYKYGYVTKDEYLSGNIRDKIAIVDSYLSKLRQTERELPH
                     LGFVENGKEKELISYEMNRLEYQKAELTKVLPKELEASEINVRLGATWIPIKDIEKFI
                     FETLKTPGYARWDIKVKFSNITSEWNVEGKSRDKGNDLAEMTFGTSRVNAYKLIEDAL
                     NLKETKVFDQIINPDGSKTSVLNKKETMLAGQKQELLKEEFKNWIFNDQERRNRLVKL
                     YNERFNSIRNREYDGSNLSFEGMNTEIDLRPHQRNAIARSLYGGNTLLAHVVGSGKTF
                     EMVASAMESKRLGMCSKSLFVVPNHLTGQIGREFMQLYPSANIMVADKKDFEPKNRKR
                     FIGKIATGEYDAVVIGHTQFEKIPMSKEYQEKHIQDQINEIVNYVEEYKHDRNQNFTV
                     KQLEKTKKKLETRLEKLNDDFKKDDVITFEELGVDMLFVDEAHNYKNLYLYTKMRNVA
                     GIGQSEAFKSSDMFMKCRYMDEMTGGKGIVFATGTPVSNSMTELYTMQRYLQYESLKK
                     NHLEHFDSWASTFGETQSSFELSPEGTGYRVKTRFSKFYNLPELMSMFKEVADIQTAD
                     MLNLPTPEANYEVIKTEPSEEQKEILKSLSERADDVRNRVVEPDVDNMLKITNDGKKL
                     ALDQRLINPLLPDNPDSKVNVCVKNVFAIWDKTKENRSTQLLFSDMSTPKGDGEFNIY
                     DDIREKLVAMGIPKEEIAFIHEANSDKQKDELFAKVRKGEVRILMGSTQKMGAGTNVQ
                     NKLIALHDLDVPWRPADLEQRAGRIVRQGNENEKVNIYRYVTENTFDAYLWQTIENKQ
                     KFISQIMTSKTPVRVAEDVDESSLNYAEIKALATGDPKIKEKMDLDNEVTKLKMLEAN
                     YKSNRYRLEDKVAKNYPEEIARTEKLIEAVKKDIASVEPKAEGEEKFTSITIAGEKIN
                     DKKLAGERLLEAISKVKINESKVIGKYRNMDLEVSYNFFTNEHNFSLNGAAKHSGELG
                     TSADGNITRLDNALEKMPEKLNRLEEKLISTKEQLQNAKEELKKPFEKADELKTKVLR
                     LAELNKLLDMGEVEEKRNDNPLVEDVKMAIIDFCNREYEENHSYDEFNTLYPDLKHIG
                     IAYTNTPDERHGIQYELDLEEKTWTQYIDGTPIKTESFYYENKGENEALRNMKNEIEL
                     FSFNDLVYVDSEDLKAALGLDIDDEGNFYDPLSKDLDNDGIADRYDNDFRDSDYFEST
                     YDVEDNLNTKVESVTKAEDKPSILSQIRAYQNESKTEEKQKTKEQEILR"
     gene            23855..24514
                     /locus_tag="KPK87_000884"
     CDS             23855..24514
                     /locus_tag="KPK87_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2537058.1"
                     /translation="MDYKTMINQIEDMVNYNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLREQGQIKDLPYVQEEKDNLINIVGNIVGEVDVVEREN
                     KNGEAFKVANFSVVSKDDEGNKVYHNCSAYGGKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHRNIRILSSKLLKEKEQMKGQEEKKESVLGAIKKYQAEDKEKPKEKKEANQEAE
                     R"
     gene            24655..24867
                     /locus_tag="KPK87_000885"
     CDS             24655..24867
                     /locus_tag="KPK87_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537059.1"
                     /translation="MRAFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEEIVECIRIDK"
     gene            24888..25916
                     /locus_tag="KPK87_000886"
     CDS             24888..25916
                     /locus_tag="KPK87_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HBG2537060.1"
                     /translation="MSKEKYYMNLPKDLSGSIAKNRFRLELLWGISKIIDEHKANNEY
                     TIIFDFKCDIELHKEDELDFYQIKTKKSGNYNSNNLCKKGKNKNNSILGKLYALYSPN
                     YNIKLAIVCNKQLKINNKEIDFPEQCFGDLDQDVLDDVRKKLCTELNLDTVCLDNVFY
                     IFDNMDLLNPEDSIRGKLVKSFVDIKGEEPQNPNALYRLVVDSVKEKASYEFDSGTYE
                     DVVKNKGITRSEFDKMLNAHKKESKNGINETQEYINNLSFAKRRRYNTALGNILETQQ
                     SESLRLIKIKIYNYIVEHENSLDDIESYLKEISKLFDDDFDVEFTDDMKSVQYIMIYY
                     MYASGGIL"
     gene            25916..27598
                     /locus_tag="KPK87_000887"
     CDS             25916..27598
                     /locus_tag="KPK87_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG2537061.1"
                     /translation="MNVIFDKIYIFDVVKKKAYSTEFKKGVNIITSSDIDGTDRGKSV
                     LLRSLYHALGADSHFDSKWGEKDKVYILFFKVDDKDFSIYRSQKLFKIFNAKGDLIFT
                     TIHRSELADFFGNLFNFSICLPQKNTNQLVIAPPAYSFLLNFLDQDEYIGTKFNSFKN
                     LAQFSNFKLNVIYSHLGIYNKEYFELVKHKEELELEIKSKKEEIEDLIKMKDRTILIL
                     DGFSCPETSSALENELNIETQKYSSLINEMNLVRNKLVDLRNQLVEQNITLSQISKFE
                     IKEEKEIKTILKSKVCPECHSVLDSTLELRSKRYNHIDNALSLKDAIKTENAHIEDEI
                     LKNEGIYSNLTLVLEEHNKKICKNKKEIKDYIKFRGLNKLIDEINNDLTINSRVIDST
                     KEALKPLKKEFKSVKDKIKSVDENYYYLIDKLKIRFNLNELEADSYEQLSKNFCASGS
                     NKPLSTVIWYLTLNDLKQKFNPEGTTFPMVFDSPNNAETDQEKKQALVEYIMESSDQF
                     SQLIVSAIGFSEKDYSIDSNINIKVLENEKYSLLNSEMYVQNYELLQCMNDA"
     gene            complement(27625..28956)
                     /locus_tag="KPK87_000888"
     CDS             complement(27625..28956)
                     /locus_tag="KPK87_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG2537062.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGEKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPEMAHQIGMELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYHSNKKSYHQIRYQSDKLCKENNLSVIDKFYESYKRK
                     YKTNGKSWYENEQSKKGTSWKSKLQFDIDRLIKQSKDWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKQRFTRAKTIGEDYTEERLKERITENQTIKTPPVKKRIGNVIDMNTNVKVKE
                     SKGYEYWATKHNLNTMAESVIYIREHGIKSVKQLDEYIQKVADERQNLQDKIKIIDKE
                     MQELSTTMEKVHTVKKYKQYYMEYKANPSDKAFFEEHKAEITRYETALAKLKKSYSKL
                     PDSKGILDKLDKLQEKKNTLMQEYSSTKSTMDELYQIRKNYGIYMDKEMER"
     gene            complement(28962..29312)
                     /gene="mobC"
                     /locus_tag="KPK87_000889"
     CDS             complement(28962..29312)
                     /gene="mobC"
                     /locus_tag="KPK87_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG2537063.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATNNINQIAKRVNSTGVIYKDDINDIKNEIEHFSKELW
                     QIHSLLLNRTSGGD"
     gene            29621..30778
                     /locus_tag="KPK87_000890"
     CDS             29621..30778
                     /locus_tag="KPK87_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG2537064.1"
                     /translation="MKKIVSRGACLLLALCLSLNAVGCQKSNENNKIKEGQSISSEEA
                     GMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYIADKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSAKEWKQVIEKELEKIKNK"
     gene            30868..30963
                     /locus_tag="KPK87_000891"
     CDS             30868..30963
                     /locus_tag="KPK87_000891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2537065.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            30956..31846
                     /locus_tag="KPK87_000892"
     CDS             30956..31846
                     /locus_tag="KPK87_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2537066.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLLQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRSAIGFLYYWKGIILIVTLLLSIIMY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            31865..33142
                     /locus_tag="KPK87_000893"
     CDS             31865..33142
                     /locus_tag="KPK87_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537067.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQEIYTGLSSDFSENIVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            33152..33808
                     /locus_tag="KPK87_000894"
     CDS             33152..33808
                     /locus_tag="KPK87_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537068.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            33808..35184
                     /locus_tag="KPK87_000895"
     CDS             33808..35184
                     /locus_tag="KPK87_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537069.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFDNITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            35282..35935
                     /locus_tag="KPK87_000896"
     CDS             35282..35935
                     /locus_tag="KPK87_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537070.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFINLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            35932..>37000
                     /locus_tag="KPK87_000897"
     CDS             35932..>37000
                     /locus_tag="KPK87_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2537071.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAV"
BASE COUNT        14154 a         4709 c         7418 g        10719 t
ORIGIN      
        1 ccgaaaaact ctattataca gctctgattg gatatatctt ctatgaagct ccaagggaag
       61 aaaagaactt tgcaacactc cttgatatga tagacgcttc agaagttaga gaagatgatg
      121 aaacatatat gaatcctatt gataggttat ttgaagcatt ggaaaagaaa gaacctacgc
      181 actttgcagt aaagcaatat aaaaaataca aacttgctgc cggaaaaaca gcgaaatcta
      241 ttcttatctc ttgtggtgca aggcttgctc catttgatat tagggaactt agggacttga
      301 tgggggaaga tgaacttgag cttgatacac tcggagatag aaaaacggca ctctttgtta
      361 ttatctctga tacagatgat acctttaact ttgtagtgtc tattatgtat tcgcagttat
      421 ttaatctctt atgtgataag gcagatgatg tatatggcgg acgattacct gtccatgtga
      481 ggtgtctact tgatgagttc gctaatatcg gcttaattcc taagtttgag aagttaattg
      541 caacaatccg ttcaagagag atttcagcaa gtataatact tcaagcacaa tctcagttaa
      601 aagcaatcta caaagataat gcagatacaa ttgtaggtaa ttgtgatagc accttgtttc
      661 ttggcgggaa ggaaaagact acattaaaag agctttctga aacacttgga aaggaaacga
      721 tagaccttta caatacctcg gaaacaagaa gcaatcaaaa gagctttgga ttgaattatc
      781 aaaagacagg aaaggagcta atgagccaag atgagataac agtaatggac ggtggaaaat
      841 gtatatttca gcttagaggt gttcgacctt ttctatcaga taaatacgat attacaaagc
      901 ataagaatta caagctcctt gaggactatg acaagaggaa tttgtttgat atagagagct
      961 acattaagcg taaagggaaa gtgaaaatga atagacatac tgtaattaca agattgtaac
     1021 atttttctta tcactatatg tagatgtata aatttggtat aatgttgttt aaataaatga
     1081 tattttatgt atgaaaccta taatatttgt actaatactg tggggtagga aaagaaaact
     1141 tcctatatac taaaatcaac cgaaagaaag gaggaaatta gaatgaatat tatcaagtca
     1201 tttaacagta caattgatta tcttgaaaca gttcttgatg atgaaattga tgaaaagaaa
     1261 gtaactagaa tatccggata ttcttattcg atgttcagtc gcttgttttc tattctaact
     1321 gaaatgacac tttcagaata tttaagaagc agaagattaa cggaagctgc gattatatta
     1381 agagatacag atgaaaaaat tattgatgtt gcattcaaat ttggatatga gtcatcagat
     1441 tcgtttggga ctgcttttaa gaattttcat ggatttactc cttctgaggt gagaattgga
     1501 aaaccgttta aattagtttc cagggtgcaa ttagcattaa gtgtaagagg aggaagaagt
     1561 atgaatataa ggattcaaaa gaaaaaagca tttacagttg caggtgtaaa tcaacaaaac
     1621 atcaattcat caatatgtgc aagcctatgg caaaaactat tttcaaaggt atctcatgaa
     1681 gaaatgttaa acttagggac cggagaagtt gtaggagtat gccatgatat attaagtctt
     1741 gataatcatg atgttttaga tagtaacacg ataaattata tggcaggata tattgttaca
     1801 gatgtagata aggcaagaag tatgggacta gatattttag aaattggaga aacagagtat
     1861 gcggttgtag agttaacagg tagtgttcca gagtgtattc ataacggttg gaagtatgcg
     1921 atggaagtgt tctttcccga gcatggatat gttcattcag gaaagccgga ctttgaatat
     1981 tattatgaag gagatatgta tagtaaagac tacaaaatgg aactttggat tcctgtatgt
     2041 aaatcttaac aaatttcagt ctatagtttt aaaacaaaat atataaaata caaggtgata
     2101 gaaagtaaaa gctatcgcct tttttatgta aaaatttaag gagatagaat gataaggata
     2161 agaagtccta cttaaaggaa aatcaaatag caagagtata agcgattggc aaagtatgaa
     2221 gtccggtcgc ttttttaaaa gaaaaggaga agttttaatg aagcaagaaa tgattaatat
     2281 taatgccaac ttgttggcag aaccgacttt ctcaaaattt gaaaaagagg gagaaaaagt
     2341 ggaagttgca aacttcacgc ttgtaaaaaa gtatgggaag ggcaaggaat atatcaattg
     2401 tgcagcctat ggtgaaaagg cgaaaacagt aaaagccttt gaaaaaggcg atttgatcca
     2461 tatctttggt tactttaaga agcgtgaaaa agacggaaag acttacaaaa actttgttgt
     2521 aaagtcatat aacaagattg aaaagaaaga agaaaacgag gaggaataaa tcatggaatt
     2581 ttttacacag gcggtaaatg tattaaagat tttagttatg gcagtaggtg caggacttgg
     2641 agcatggggc gttattaacc tgatggaagg ttatggtaat gacaatccgg gtgcaaaaag
     2701 tcaaggaatc aagcaactta tggctggagg tggcatagta cttatcggat taaagctgat
     2761 tccacttctt gcaaatgtac tcagataaga agaaaaggag aaactaaatg tttgggatat
     2821 tcgacaaaat agaggagttt ttcaaagagt ttctattagg cggtatccaa gcaaacttag
     2881 agtctatgtt tcttgacatc aacgataaag ttggtgcgat tgcaacggat gtaggaaaga
     2941 caccgatggg gtggaacgga caggtcttca gttttattaa aagcattaac gattccgtta
     3001 tcatccccat agcggggcta atcataacgg cagtcctttg tatcgagctt atcaatatgg
     3061 ttatgcaaaa gaacaatatg cacgatacag atacgtttga tttttttaaa tacatcataa
     3121 agatgtggat tgccgtatgg ttagtatctc atgcctttga gttttcaatg gcagtttttg
     3181 atgtagctca gcatatggtt aataaagcag caggagttat aaacacatca gcaactgttt
     3241 ccggaaatca gattgtagca atgattgatt ctctaaaaga caaaggtctt ggagagcttg
     3301 tgatgatact ctttgaaacc tctctcataa aggtagctat acaggttatt tccattgtga
     3361 taatgcttgt ggtttatgga agaatgtttg agatttatat ttactcatcg gtttcagcca
     3421 ttccatttgc cacaatggga aacaaagagt ggggacagat tggaacaaac tatatcaaag
     3481 gattgtttgc cataggttta caaggactct ttttaatggt ttgtcttgga atatacgcag
     3541 tattagttaa gacaataaag ataacagaca tacacacaag tacctttacg atacttggat
     3601 atgcggtttt gctagggtta atgatgttaa agagcggaac actggccaaa agcgtattaa
     3661 atgcacacta aaagaaagga agggtaatat gaacaaaaga aaaacagtat ttatagtagt
     3721 aattggagca ggtattatgg cggtgataga taggatcaga cttcacagca aggtaaatga
     3781 cttggaagaa agaaaaaaag acatcggtcg ctgccacaat gatttttgcc taatgcagga
     3841 aagatttaac agaagtatag atgaacagat tacaagtatt caggaagaaa tcggctccgt
     3901 atatgagcat atcgaggagc tttcaaaggg aaaagaagat gggaggtaag ttatggcgta
     3961 tgtacctatc cccaaagact taaagaagtt aaaaacaaag gtggcattta acttgacgaa
     4021 aagacaactg ataggtttta cgattgcagg acttattgga atacctatct atttatttat
     4081 gcgaaaggtc gttcctaatg atatagcagt catatttctc attgtgtcca ctctccctat
     4141 atttttcatc actctttttg aaaaggacgg attgacattt gaaaaatatt ttaaatatat
     4201 ttaccttcat aagttttatc agccacaaaa aagagtgaga aaggaggttt accttgaaca
     4261 aaaaaagaaa gattcagcag ctaaagttag aacaaaacaa aaagaagtta agaagtcaaa
     4321 gacaggactt aaagcaaaat aagggaaaat ctaaaaaaga taaaggcggg ctacttgacc
     4381 ctatctttag gaaagaacca aaaagatata ctgttgaaga taccattccc taccttagac
     4441 tattaaaaag tgggatatgt cagattgatg aaaatcattt taataagagt atcgcctttg
     4501 aggatataaa ctatcagctt gctttagaag aagataggga tttgattttt aatcagtttg
     4561 caaactttct taattccttt gatccaagta taagcataga gttttcatat atcaatcagc
     4621 ttggcagaaa tgaagaaatg aaatcggcaa ttcaaatacc ggataaagga gatggttttg
     4681 acgatatacg tcttgagttt cgagaaatgc ttaaaagcca gattgtaaaa ggaaataacg
     4741 gactgaaaaa atcaaaatat gtaactttta cagtagaagc ggataattta gaacaggcaa
     4801 catcaaaact ggaaagaatg gagatagata tattatcaag ctttaaaagt atgggagtaa
     4861 gagcagaaag ccttaacggg gaggaaaggc taaagatact tcatgatgtt ttaaatccga
     4921 ataagacctt tgaattttca tataaggact taaagaaaaa ggaaagtaca aagacatata
     4981 ttgctccaga tgaatttaac tttacaccgt caaggtattt taagtttgga aattttatcg
     5041 gagcagcaag tcattttcaa attcttgcaa gtgagctttc agatcgtatg ctgtcagagt
     5101 ttttggatat agatgataac atcaatatct cttttcatat caaggcaatt gagcagtcag
     5161 aagccatcaa gatggtcaaa agaaaaaata ccgatattga caagatgaag attgaggaaa
     5221 acaaaaaggc tgtaaggtca gggtatgaca tggacattct tccaagtgat ttaattacct
     5281 atggggaaga tgtgaaaagc ctattaaaag acttacagac aagagatgag agaatgtttg
     5341 ttgtaaccat catttttatg aactttgcaa ggacaattca aaagctggat aacaccattt
     5401 ctcaaattag ctccattgcc aataagcata attgcaagtt aaaaaggctt gaccactcac
     5461 aggagcaggg cttaatcagt gtacttcctc ttggggtaaa taagattgaa attgatagag
     5521 ggctaacctc atcatctacg gcggtcttta tgccgtttac cacagaggag cttttcataa
     5581 actcggcaaa cagtttgtac tatggactaa atgccctaag tcataacctg attatggcag
     5641 atagaaagaa gttaaaaaac ccgaacggtc taatccttgg aactccggga agtggtaaat
     5701 cctttagtgc aaaaagagaa atggcaaatg ccattcttac aaccgatgac gatgtgatta
     5761 tctgtgatcc ggaaggcgaa tacggaaacc ttgtaagaca atttaaggga gaagtcataa
     5821 aggtcagcag taagtcgaag gactatctca atcctttaga tataaatatg aactatggag
     5881 atggtgacgc accactaaag gacaaagcaa acttcattat gtctatgctt gagcttgttg
     5941 taggaggtag tggtcttaca gcagaagaaa agtcggttat agatagatgt ctgcctaaga
     6001 tttatggaaa gtattttgaa aatccaattc ttgagaatat gcctattctt caagacctgt
     6061 atgatatgtt aaaaaatcag gaagaaaaag taggtaaaaa gttggcaaca gaaatggaga
     6121 tttatgtaac aggatctctt aatgtattta ataacaggtc aaatgtggac ttaaataagc
     6181 agcttctttg ttttgatatt aaggagttag gctcgcagct taaaaaaatt ggaatgcttg
     6241 ttattcagga tcaggtgtgg aacaaggtat cgcaaaacag aggaaataag gctacaaggt
     6301 attatataga cgagtttcac ctattgttaa aagacgagca gacaacttcc tattccgtag
     6361 agatttggaa aagatttcgt aagtggggtg gaatcccgac aggcattaca caaaatgtca
     6421 aagacttact tatgagtaat gaaattgaaa atatctttga taatacggac tttgttttaa
     6481 tgcttaatca ggcttcaggc gatagagaaa ttttagcaag gaaacttaaa atctctcagc
     6541 cacagctaaa atatgtaact aattccaatg caggagaagg acttttgttc tttggaaaca
     6601 ccattgttcc tttccttgat aagttcccaa aagacacaat actttatcag aagatgacaa
     6661 ccaagcctga agaagtgagg taggcttatg ggaaagaaac tgaaaaagga ctttaaggaa
     6721 aggcaaaggg caaggattga aaaggaaacg ctgcaaagta atatgggaat tgagcctgaa
     6781 gaaagtaagt taaagtataa cgatgattac agagggaaaa ttgttcatga taaagacaga
     6841 ttccaagata aggtgcatga gaaaacaagc aaaatgacct ctgacagtga accagttaga
     6901 gggacttcca aaagaaatgc aagatataga gcttccaata aggatagcat aacttcttca
     6961 actgaaactg gaggtgtaag tcaggaagaa atcgggaaag cggattatgg tacggaggtt
     7021 aaggatggaa aaatctatga tcctttagga aaagaccttg ataatgacgg aatcatagat
     7081 agatatgaca atgattttag ggacagcgac tattttgagt caacctatga tgtagatgga
     7141 ttaaaaaaag atgagattag cggaaatccg gtaaataagc aaaacactca aaagaaaaat
     7201 tataagagaa aaaactattc cgataagctc tatacaagaa agaaggacaa agaagctaaa
     7261 gaggaaaaag cagataataa aaaaagcgga aaagaagctg ttcttgatag agaaaagaaa
     7321 aacaggcttt ctaaagaaca ggaaacaatt ttcgagaata agacttctaa agtatctgct
     7381 ttatcaggct tagcaaaagg aagcgaaacc gtaagagatt atttatctca tggaagtgat
     7441 gaaaatcaag gggtagaggc aggagaaaag acagcagata caagctcaaa actcattcat
     7501 ggcattaaga actactagga taagaaaaga acaaagaaag gatatgacct tacaaataag
     7561 gactataaaa tcagaaagcg aaaatccaaa ttagagtttc gagatgccaa agaggaactg
     7621 aaaaagacgg aggagtatca aaaagcaaat aactttaaac gatttcaaaa aagaaagcag
     7681 atgaaagatt ccattaaaag gcagaataag tcaaggctta gagatcgcat taaggaaggg
     7741 cttatcggca gtcttaaaag ttcaaaggaa gttatattaa gaaaaacgaa gggacttatg
     7801 attattttca taggtcttat tcttttggga actttcttta ttaactttgc agggacagga
     7861 atgacaggct ttatgaactc tacaagtaca gttcttacaa caagttattt atcaaagcct
     7921 aatgttctca cagaaattca tcaacaattt tctagtatgg agcaaggatt acaagatgag
     7981 cttgaaagtg ttgaagaaaa ttatcccggt tatgatgagt atattgtcaa taaagaggga
     8041 gatattggtc ataataccca tgagctttta tcctacatta catcaaggtt cggagaagta
     8101 aagaatgtat cagaagtaag ctctgtatta gatgagcttt ttaagtccat gtataggctt
     8161 gagtataagg aagaaattga aatcagatac aggacagtta cagaaatcta tactgatgaa
     8221 gatggaaacg aatataccga aagccatgaa gaaccttatg agtacaagaa actcattgta
     8281 actctttata aaagagaaat ggatagtgtt gttcgagaag tgtttcaaaa ttatccagat
     8341 aatgtaaaac attatgaagc attacttgca agtcttggta atatgagtga cttttttgga
     8401 agtggaagta atgattattc tgaaataata aacaatccta attttggcaa tccgggaata
     8461 gctttcaatg aagaaacggt taaacgactt ttctcagaag cagaaagaca tataggaaag
     8521 aggtatgttt ttggtgcaaa tgggccgtca aattttgact gctcttcatt tgtgtgttgg
     8581 agctttactc attcaggtat taaaaatatg ccaagaacaa cagcttggag aatttatacg
     8641 gattactgta atcctgtaag tacaggtgaa gccaaggcag gagatataat attctttaaa
     8701 aatacctata atagtggaag cccgatttct catgtaggta tatatgccgg aaatgggatg
     8761 atgatacacg caggggatcc tattcaatat acttctatta actcaccata ttggagagaa
     8821 catttttatg ggtttggaag accaagatag gaggatacaa tttgaaaaaa gaactgacag
     8881 cagtaaaaaa tagaataaag aaattgaaag ataaaaaggc tctgattgat gaagaattgg
     8941 agcctttgtt cattcgtgaa gaagaacttg aaaacgaaga aattattgcc atttgcagaa
     9001 aaaataacat tacaatttct gatcttatgg caaaagtaaa cagagaaaaa tcagaaatga
     9061 aaaaggagaa cggaaatgaa aaaaatgaag aataaaaagg ctgcgggagt aattcttgga
     9121 gcagtgcttt tgatagtggg agtagtaatc tctttatttg caggtagtaa gattgtattt
     9181 gcacaaggag ggttgtttac gacacttgta aatccgatag aagccgttgc tcaaatagaa
     9241 gtagaagtta atgttaagta tatttttgaa gatgaaaaag tctttaagga ggaaaagatt
     9301 aaggctgaaa aggagtggct tcttgacagt ggagatcttc caataattcc tgatgatatg
     9361 aaatttatag atgaatttct attttatgaa gtaaagggag acgggaaaga tgaaattatc
     9421 cgtaaagtta caaagattga tgttaaggat aaggaaacgc aaacagaaaa tgaaagctca
     9481 aaatctgagc aaagctctca atcaaatgct tcaaagatag aaaataaggg aactcaaaca
     9541 gagcttagca aagatgatat ttctaaaatg gaaagggagt ctaaggaact taaagaaaaa
     9601 cttgataagc taaatcagga gattaaggac aaggataaac taagcgagaa gcaaaaggat
     9661 aaaatcaaag accttgagga gaaaatagat aaactgaagg aaaaacttga aaagggcaaa
     9721 gatgataagg acttatcagc agatatgaaa aaggagatag acaagctaac cgataagatt
     9781 aaggaacttg agaaaaaggc taatgaggtc ggtagagctc ctattatgca taatcctaat
     9841 gtaacaccaa ttagccctat ttccggaatt aaacaaggaa caggaaatgt atcaccacaa
     9901 acatctataa atagcagtgg taaaggttct gccacgcagg aaagtaaagg ggcaaataca
     9961 aaagatacaa acagcaatga atcaaaggag aaagaaaagc aagttcgtta tccaaataag
    10021 ctgacaccaa aacagcctgc taataatggt agtcacgatt cttctctgga tggttcaagt
    10081 aagaatataa ataccaacaa aggagttgct tcagaacctt caaaggcacg aggaacagtt
    10141 actgaaaata aggataatgc aaacaaaaat tatcctattc accataatga cagtaaagat
    10201 aataaagaaa cggataaata ttctgctgat gcaagacagt ttgttacctt tacaactaaa
    10261 aatggtaaga cctttcattt aatcattaac catgatgagg atagcgagaa tgttatgctc
    10321 ctaactgaag tatctgaaga tgatctctta aatatggtag aaaagaaagc accggagaaa
    10381 gagattgtta aagaagaatc acaaaaagaa gagcctaaac aagtgaaaaa agaagaaagc
    10441 agtaatacag gaacctactt aattctcatt cttgtagtag cgggagcctt aggtgcagga
    10501 tattatttta aggttattaa gaaaaaagaa gataaggagc ttgaaggctt tgaggaggaa
    10561 gatgatgatt tcttttcaga ggcagaagaa agcgaaaatg aagtaaacga agcagtaaca
    10621 gaagataaag aagatgatga gatagagtaa acttgtcccc aaaattatat aatatttggg
    10681 gcaaaacaca gggcgagaga gtaaaatctt tcgccctctt atattgaaaa caggaggaaa
    10741 actatatgaa acttgtaata gcagaaaagc caagtgttgc aatttctatt gctaaagtaa
    10801 ttggagcaac aaaaaagaaa gacggatatt atgagggaaa cggatacaag gtaagttggt
    10861 gtgttggaca cttaattcag atggcaaatc cggaaagtta cgatgaaaag tacgctaaat
    10921 ggaatatggc ggatttaccg attattccaa gagaatataa gtatgagatt gcaaagtcca
    10981 caaagaaaca atttacgatt cttaaaaagt tgatgaatga caaggaaata gacattgtga
    11041 taaatgcttg cgatgcagga cgtgaaggag aagcaatctt caggcttgtc tataatcagg
    11101 caaattgcaa aaagaagatg aaacgccttt ggatttcatc aatggaagat agtgcaatta
    11161 aagagggctt tgataaccta aaagacggaa gtttttacga taatctcttt gaatctgcac
    11221 aagcaagagc gattgcggat tggcttgtgg gaatgaattt aagtaggcta tactcgtgcc
    11281 tatataagca gaattattct gttggcagag tacaaacacc aactcttgcc atgattgtaa
    11341 aaagagatga tgaaatagca aatttcaaga aagaaaaata ttttacagta gagcttgatt
    11401 tagacggctt ttctctttct accgatagaa ttgatgataa gacagttaca gagcagctta
    11461 tcaatctatt aggtaattcc atagaaatta ccgatgttat tcaaaaggaa aagataacaa
    11521 aacctgagct gccatttgac cttacaacgc ttcaaagaga gtgtaataag tatttaaatt
    11581 atagtgcaaa gcagacctta gattatacac aaagtttata cgagaaaaaa ctgattacct
    11641 atccaagaac ggacagtcgt tatcttacag aagatatgat tacaagtaca atcaacaata
    11701 ttattggaaa gcatgatttt gatacaggtc gcattaagat ggtatttaat tctaaaaggg
    11761 ttacagacca tcatgcgatt ataccgacag caagcagtat gaatgaggac ttagcttcac
    11821 ttccggaaag tgaattgaag gtatatgaac ttgttttaaa taagctacat gcaagtgtag
    11881 gctatccttt aattgagaat actacaaaga ttgtagctgt atttgacgga tttgaattta
    11941 caagcagtgg aaaggtgatt gtagatgagg gctttaccaa atacctaaaa aaatatataa
    12001 aaaagaaaaa tgaagatacc gtgtttccgg atttaaagat tggtgatagt cttgatatta
    12061 aggagaaaga gataaaagaa aaatacacat caccggctaa acattttacg gaagatacac
    12121 ttttaaagga tatggagctt gcaggaaatg acgcactgga aaaaggtgta gaagttgagc
    12181 gaaaaggact tggaacacct gcaacaagag caggcattat tgaaaattta atctttaagg
    12241 gttttgtaga aagagataag aaaaacctta ttgctacaca caaaggaata agccttgtaa
    12301 cgattgtctc agatactttt aaatcagcag gtacaaccgc taagtgggaa atggagttat
    12361 cggatattgc acaaggcaaa gcttcaaagg aaaatttttt aaaagcgatt gaaaacgaga
    12421 taaaagaatt ggtttcaact tatagcaagt aagatagcac taattttaat atcgaaaaaa
    12481 tgtcctaaaa atgctataat ttttgtaggg aaagtaggaa atatggaggt gttttatgtc
    12541 ggaagtattt atgaataatg caaaggttat ggcaaaggga caggttacta ttccaaagaa
    12601 aataagagaa atattagaaa tcgagaatgg agattatgta acttttgttg taactgaagg
    12661 gaaaatacaa attgttaatt caaaaacttt tatagagaaa aatatacagg gcaggaaata
    12721 aattatagat ggcagatgca gataggagga atttatgaat aaagaaaaga attctttaca
    12781 aaatataaac atagaaaaaa acaacaattc atttgaagaa attgttatga ttgtagaaaa
    12841 tgcaaaggat cgtgcatata gaaaagttaa tgaagagcta atcttaatgt atcaagaggt
    12901 tgggaaatat attagtgaaa aaacaaagga agcatcttat ggttcaggat tcgtagataa
    12961 tgtagctgaa tttttttcta ttaactatcc tgatttaaaa ggatttaatc gcagaggcct
    13021 ttatagaatg aagcaatttt acgaactgta caaagatgat gaaaaagtgt caacgctgtt
    13081 gacacaattg agttggtcta atcatttaaa aataatgtcg ggagctaaaa gtagagaaga
    13141 gagagaattt tatattaact tagcaattaa ggaaaatttg acacatcgag agctggtaag
    13201 acagatggat agtggatact atgaacgctg tatgctttca aatgagggaa atatgcctgc
    13261 aatacaaaga gcgaaacagg aaacacataa tttgtttatg gatagctatg tattagaatt
    13321 tcttgatgct cctaaaattg gaaatgaaag agaatttcag aagtcaattc ttgaaaattt
    13381 gaagaacttt gttttagaaa ttggaaaaga tttttctttt ataggtaatg agtatagagt
    13441 gcaggttgga aatcatgact actatattga tttattattt taccatagag gtttatcctg
    13501 cttggttgct tttgaactta aaatcggaga atttaagccg gaatatgttg gtaaaatgaa
    13561 cctatatcta gaagctttag atagagaagt taagaaacaa gcggaaaacc caagtgttgg
    13621 agttattctt tgtgcatcaa aagatgatga agtagttgag tttgcactaa gcagaagcct
    13681 ttcgccaaca atggtatctg aatatagatt aaaattgata gataaagaac tattacaaag
    13741 aaagttaaaa gagtatacag gaattgcaga gcaggagata gaaagacatg atgaaaaata
    13801 gataaatata actttgtgca tcaaaaaatg agtgaagtag tccatgtgga aacaataata
    13861 aaataaaagt gtttcaattt agtaatatac tatgaaagga tatgaaaaga aatgcgtaca
    13921 ggcaatgaat ttaacggtgt tgaggatact ttatatatac ctttagctgc cagaatatat
    13981 gtatctgaaa aattcccaca ttttttctat gatgaaaagg ctctatcttt gaaacccaag
    14041 attccaacag gcggtataga taacaatagt acggaatatt tttatatggc tagtgtttgt
    14101 agacaacaga ccatagatat gaaaattaaa aaattccttg atgaaaataa aaagggaaat
    14161 atggtattct taggagcagg tttagagact tcatataatc gcataggaaa tcaaattgta
    14221 aatttctatc aagtagatct tccgggtgta atagaagtac gaaaaagagt gcttggaaat
    14281 gctaaaaatg agaaacttat atcaggagat atgtttgacc ttaactggat taaagaaata
    14341 gatacttcat tgccgacact tatttctgta gcgggtgtat atcagtattt tgacgaatat
    14401 aaaattatag atatgattca aaaattgaaa gctcaatttt ctaagggaga attgatattt
    14461 gacgcaacaa actctaaagg tttgaagctt gctaataaat atgtgaagaa aacaggaaat
    14521 aaaaatgcac aaatgtattt tagtgttgat aattctaaag atttagcaga taagactgga
    14581 acaatactat tggaagttga tggatttttt catggagcat tgaaggcttg tcagggatta
    14641 aaactaatga cgaggttata tatgtatttt gcagataagt tgcatcgaac attggttata
    14701 catttgaggt ttaattaaat tctattcaat tagttcttat tacttgtatt caacgtataa
    14761 tatatagaat caaaaggtgg ttagagaaaa tctaattgcc ttttttattt gtaaaaaaga
    14821 aggaggaaat atgcgaataa atgattttca taacattttg gagcttgtaa aacaggatat
    14881 tctgcaaagt gaagcagaat atctgaagct cttaaaggtt gtcggaaaca atcagagata
    14941 tgactttaga agtcaactta gtatctacga taaaaatcct gaagccacag cctgtgccaa
    15001 gttcgactac tggagagaac gctttaaccg aacagtaatg cgaggtcaaa agggtatccc
    15061 tattttagag gactacggca catacaaaaa agttgactat atctttgata taagccagac
    15121 agtttcaaag aatagggatg tcaacgaggt taatctttgg agatttgata aggaagctca
    15181 caaagatgta ttaaaagaaa tgataaaaac tgaaggctat gaagaaagcg aaagcacctt
    15241 agaaaatatc ttttctttaa gcaggctcta cggagatgaa aaaatagata acctgatgaa
    15301 tgagcttaga atagctgacg aggatagaat ttcctttact aaatttgtga gggattcgat
    15361 aagctatgca gtagcttcaa gatttaagtt agactatcct atggataagg agctgttaaa
    15421 ggaaaacttt gcaatgcttg acagcatttc tcttatgagc cttggagaaa cggtgtcgga
    15481 tattagcgga acagttattg atacgactat tcaaaaaagc aaagagttag agcttaaaaa
    15541 agaagtttta agaggtaaag aagcaggata taataaaata aaagaagaat tagaggaggt
    15601 agaagaaaat gtacttcgac gagatgatca ggaaagaatt agtgaaaacg agcgaatttt
    15661 ccgaaatgga aagtacgggc gagataatag agaaaatcaa ggagaataca ctgaatcagt
    15721 tagaggaaga gatggacttt atgagggaat atccgaatcc agtgtacgca gtgatgaggc
    15781 tgaattatct ttcaaacagc gaagagcaga gccacttcga gatgttagtg gatctataca
    15841 aggagaagaa gctgaccaaa cacctgatgg atactcagaa acaggtgata gagtttatga
    15901 gagccgaaaa gcaaaaactg atgatgtctt ggcagataga gaacgagagc catcctcagt
    15961 acaaagcaat gatttcagcc ctcaaagaaa tgacaatcaa ggaagtagtg gaaatctaaa
    16021 agagaatacg gataaagaga taagggaagc tgataaggct tctttttcct tgcctgaaaa
    16081 cagctcagga caaattaaac ttacaatacc tcttgcccaa aatgatattg acagcattct
    16141 tgtaaacggt ggaaaccatg agggtggcag acttcctatc attgctgaat tttccaaagg
    16201 aaaaacagta gaaaaattag gagaatacct taaagatacc atgggaggcg gaaacggttt
    16261 cttccttaat ggaactgaag tatcttcctg gtattcggat aaaggcattc atttagccta
    16321 cggaacttcc gcaagagaag atatgacgca aattttaagc tggagtgatg ctgcaagaag
    16381 aatcaatgag cttttggaaa atggagagtt tgctacaaat gtagagcttt tagaagcaca
    16441 ggactatgaa agaaatagaa tttcagaatc gctatggtat ctatatcacg atttaagcga
    16501 agaagggaaa gcacagggct attttgactt tatagaaaca ggtggtggct ttccgaaaga
    16561 aacaagacag ttatcggaag ctcttaaaaa tcctgactat ctaaaagatg ttgtttccga
    16621 gtacagtcgc tttttgacag gatataaaga aaacaaagag gtactaagat ttcactatca
    16681 caaagtagat agcctttacc agagattaca ggagcttgca cttacaagaa aggaatatac
    16741 ctctaacctt atcgaacttc caaagataca gggctttatt acagaagatg aagtccttgc
    16801 tacaatttca agaggaagtg gagtcgataa aggaaaagag cgaattacta agtttttcaa
    16861 agaaaatcat accttgcagg aaaaagcgaa tttcctaaaa ggtgaatatg ggatcggtgg
    16921 aggctctcat gctgtatcag gcgaaagagg aagcggagaa tggcatgatg caaagggact
    16981 gaaattacag aagaatcatt gtaacgatgt ttttcttaca tggtcaaatg tagcaaagca
    17041 tatagatgag ctgctttcta agaatcttta tcttgaagaa aagaacattg aaaaaaaggt
    17101 agagatagaa gaacaaaaag agccaagtta ttactctaaa gacgatccat ataatctaat
    17161 tactgatgaa atgcttgaaa aagttcctga actttatgca caagaggatg tagctttagc
    17221 agacaaacag gttcatgcag catacatcat tccatttaga agtaactgga cttggtatat
    17281 gacggaatat gacagggaaa gtaaagatgc ttttggactt gttttaggaa ttgagccgga
    17341 atggggatat tttaaccttg atgagctaaa ggaattaaat gcccagaggc ttgttttaga
    17401 agattttcca aagaccttta gagaacttaa agacagtgaa cttaaaaagc agatggatga
    17461 gcaggagctt tatcaagttt ttaacggaga gctaagtttt gaggaaaata atatagaact
    17521 tgaagcacct gaagatgtag aagaaagcaa acaagcagaa ccttttcagg ctactttatt
    17581 tgactatcta aaggaaaaag aagaagtaga gcttaacgaa aaagagaaca gcctttcaga
    17641 tgagtttaca gttaaagaag gcgataccgt ctattttaat catgaggagt acaaagtaag
    17701 agatctttct aaaaatcaaa tcacaggaaa aaatgatttg tggcttgatc ctgtaaggca
    17761 aggtaatcat caaatcccta ttgtagcttt tacagataat gaggatttat taagacaaat
    17821 aagtcttgaa agacctgaat ttatcatcgg tgatgaagtt aagtacaagg gaaaagacta
    17881 tgccatcaca aaatttgata atatgggaaa taacctaaag actgtaacgg ttaaggataa
    17941 taccgaatat cttggaggta tgattacagg ctccgatgta attccttatc gccaagaaag
    18001 tgagcttaat aaaatttttg aaaatttgac ctataaaaaa gaggagcttt atgaccttgc
    18061 agtaagaata gatgagatag cttatgattt tgattcttat gactatgcgg attccgtaga
    18121 agcagaatca gagaaaagac ttgataatat caaccaaatc tatggtgatt tacaagcagg
    18181 aaatgtttca gtatatataa atagtttgaa tgagatgatt gaagaagatg ttgaagatac
    18241 ggaaatcatc aagcaagtat taacacaatt agaagaaaaa aagattgata ttcagcctaa
    18301 acaagaagta ttcaatttca agattacaga agaaacactt cctcaaaggt tatctccaag
    18361 tgagaggtta aataataacc ttgaagcaat ctctatgctt aacagagtgg aaagtggaga
    18421 acgagagctt gatattacag ctcaggaagt tttagctaaa tatgtcggct ggggtggact
    18481 ttctgatgtc tttgatgaaa gcaaggatgg acagtggaaa gatgcaagag ccttcttaaa
    18541 ggaaaatcta tcgtcatcag aatatgaagc tgcaagagaa tcaaccctaa cgagctttta
    18601 tacaccgaaa atagttatag atggaatata taagactctt tccggtatgg gatttaaaca
    18661 aggtaatatc cttgaaccgt caatgggtat cggaaacttt atcggtaatg tacctgatga
    18721 aatgggtaag tcaaagtttt atggagtaga gcttgactcg gtaagtggtc gcattggaaa
    18781 actactgtat ccggaaagtg aagtacaggt aaagggactt gaagaaacgg gattctccaa
    18841 taacttcttt gatgtagcaa tcggaaatgt cccatttgga gagtacaagg taaatgacag
    18901 agaatacaat cgcaataact ttcttattca cgattatttc tttgccaagt ccattgataa
    18961 ggttcgtaac ggcggtgtta tcacctttat tacatcatct ggaacaatgg ataaaaagga
    19021 cgaaagtgta agaaggtatc ttgcagcaag agctgaattt ttaggagcaa tcagacttcc
    19081 aaatgatacc tttaagggtg ttgccggaac tgaggtaaca agcgatatta tattcctaaa
    19141 gaaaagagat agcgtattag agcgagatga agcttggata cacctttcag aagataaaaa
    19201 cgggcttgta tataacaaat attttgtcga tcatcctgag caggtgcttg gttctatgcg
    19261 tgaagtaagc ggaaggtttg gaaaaactct aacctgtgaa ccgatagctt atttagggca
    19321 agagataaat atggcttcat taaaggagcg tatcgagatt gcaggagaaa gaatttcaaa
    19381 agataccaag tatgaagaaa tagagctttt agatgatgaa atcacttcaa tccctgcaac
    19441 cgatgatgta aagaacttct cttatacctt gaatgatgat gaagtctatt acagagaaaa
    19501 ttcgctcttt attaaaaagg aagtatcaga caagaataaa gaaaaaatca aagattatct
    19561 tgagctgaat gttgccttaa aagatgtgat ttacaagcag aaggaagatt ttagcgaaga
    19621 agaaatcaag gcttcacagg aaaaactaaa tgaagtatat gacaactttt caaagaagca
    19681 tggctttgtc aataatttaa gtaataccag agctttgaaa gaggatagca actttccact
    19741 tgtttcctcc atagaaattc ttgatgaaga agaaaatttc aaggcaaagg gagatatttt
    19801 ctccaaaaga actattacaa aagccaaagt tatagaccat gtggatactt ctttggaagc
    19861 ccttgtttta tcggtatctg aaaaaggcta tgtggacttt gactatatgg gaagtcttac
    19921 aggaaaagac agagcgactt taattgagga gcttcgaggg gaaatctact taaatatcag
    19981 agaagaacaa aatttttaca gatcactttc ttttaatcct gaagatggag atttaccttt
    20041 tgcctgtgca aatggcagta attcctataa atatggctat gtaacaaaag atgaatacct
    20101 aagtgggaat atcagggata agattgccat tgtagatagc tatttatcaa agttaagaca
    20161 aacggaacga gagcttcctc atcttggctt tgttgagaat ggaaaagaaa aagagctgat
    20221 aagctatgaa atgaatcgtt tggaatatca aaaagcagag cttaccaaag ttcttccaaa
    20281 ggaattagaa gcaagtgaaa tcaatgtgcg tcttggtgcg acttggatac cgattaaaga
    20341 tattgaaaaa ttcatctttg aaactttaaa aactccggga tatgcaagat gggatattaa
    20401 ggttaaattt tcaaatatta caagtgaatg gaatgtagag ggaaaaagca gggataaagg
    20461 aaatgacctt gcagaaatga cctttggtac atcaagggta aatgcctata agctgattga
    20521 agatgcccta aatcttaaag aaacaaaggt atttgaccag attataaatc cggacggttc
    20581 taaaacttct gtcttaaata aaaaagaaac catgcttgca ggacaaaagc aggagctatt
    20641 aaaagaagaa tttaagaact ggatatttaa cgatcaggaa agaagaaacc gtcttgtaaa
    20701 actgtataat gagcgtttta actcaatccg aaacagagaa tatgatggaa gcaatctttc
    20761 ttttgaggga atgaatacag aaattgattt aagacctcat caaagaaatg ccatagcaag
    20821 aagtctttat ggaggaaaca cattgcttgc tcatgtggta ggcagtggaa agacctttga
    20881 gatggtagct tccgcaatgg aaagtaaaag acttggaatg tgcagtaagt ctttgtttgt
    20941 tgttccaaat cacttaacag ggcaaatcgg tcgtgaattt atgcagctat atccgtcggc
    21001 taatattatg gtggctgata aaaaagactt tgaaccgaag aacagaaaga gatttatcgg
    21061 aaagattgcc acaggggaat atgatgcggt tgttatcggg catacgcagt ttgaaaaaat
    21121 cccgatgagt aaggaatatc aggaaaagca catccaagat cagattaatg aaatcgtaaa
    21181 ctatgtagaa gaatacaagc atgacagaaa tcagaacttt acagtaaaac agcttgaaaa
    21241 gacaaagaaa aaactggaaa cgaggcttga gaaattaaat gatgatttta agaaagacga
    21301 tgtaattacc tttgaggaac tgggagttga tatgctcttt gttgacgagg cacataatta
    21361 caagaacctt tatctctata ccaaaatgag aaatgttgca ggtatcgggc agtcagaagc
    21421 ctttaagtcc tccgatatgt ttatgaaatg cagatatatg gatgaaatga caggaggaaa
    21481 aggcatagtt tttgccacag gaacacctgt aagtaactca atgacagagc tttatacaat
    21541 gcaaaggtat cttcagtatg agagtttaaa gaaaaatcac ttagagcatt ttgattcttg
    21601 ggcttccact tttggagaaa cacaaagctc ctttgaacta tctcctgaag gaacaggata
    21661 tagggtaaaa acgagatttt caaagttcta taacttgcct gaactaatga gtatgttcaa
    21721 ggaagtagca gatattcaga ctgcggatat gcttaacctt cctacacctg aagctaatta
    21781 tgaagttatt aaaactgagc cttctgaaga acaaaaggaa atcctaaaga gcctttctga
    21841 aagagcggat gatgtcagaa atagggttgt agagccggat gttgataata tgcttaagat
    21901 taccaatgac ggtaagaaac ttgccttaga tcagcgttta atcaatcccc tacttcctga
    21961 taaccctgat agtaaagtaa atgtatgtgt aaaaaatgta tttgctattt gggataagac
    22021 aaaagaaaat aggtcaacac agctactttt ctccgatatg tctactccaa aaggagatgg
    22081 agaatttaat atctatgatg atattagaga aaaacttgta gcaatgggaa taccgaaaga
    22141 agaaatcgcc tttatccatg aagcgaattc tgataagcaa aaggatgaac tctttgcaaa
    22201 ggtaagaaaa ggcgaagttc gtatcttaat gggttcaacg cagaagatgg gagccggaac
    22261 gaatgtgcaa aataaactga ttgcacttca tgatttggat gtgccttgga gaccagctga
    22321 ccttgaacaa agagctggta gaattgtaag acagggaaac gaaaatgaga aagtaaatat
    22381 ttatcgttat gttacagaga atacctttga tgcgtacctt tggcagacga tagaaaataa
    22441 gcagaagttc atttcacaga ttatgaccag taagactccc gtcagagttg cagaagatgt
    22501 ggacgaaagc tcccttaact atgcagagat taaagcactt gccactggtg atccaaaaat
    22561 caaggaaaag atggacttgg ataacgaggt tacgaaactt aaaatgcttg aagcaaacta
    22621 taagtccaac cgttacagat tagaggataa agtggcgaaa aactatcctg aagaaatcgc
    22681 aagaacggaa aaactcatag aagctgttaa gaaagatata gcaagtgtag aacctaaagc
    22741 ggaaggtgag gaaaagttta cttccattac cattgcggga gaaaagatta acgataagaa
    22801 attagctgga gaaagactgc ttgaagctat ttccaaagtg aaaatcaatg aaagcaaggt
    22861 gataggcaag tatagaaata tggatttaga ggtaagctat aacttcttta ccaatgaaca
    22921 taattttagc ctaaacggtg ctgcaaagca ttcaggagag cttggaacaa gtgcggacgg
    22981 taacattaca agactggata atgctcttga gaaaatgcct gagaaattaa ataggcttga
    23041 agaaaaactt atcagcacaa aggaacaact tcaaaatgcc aaagaagaat taaagaaacc
    23101 ttttgagaag gcagatgagc tgaaaactaa ggtgttaaga cttgcagaac tgaataagct
    23161 ccttgatatg ggagaagtgg aagaaaagag aaatgataat ccacttgtag aagatgtaaa
    23221 aatggcaatc attgatttct gtaacagaga gtacgaagaa aatcatagct atgatgaatt
    23281 taataccttg tatcctgact taaagcatat cggtatcgcc tataccaata caccggatga
    23341 aaggcatggt attcagtatg agcttgattt agaggagaaa acttggacac agtatattga
    23401 tggtacgcca atcaagacag agagctttta ttatgagaat aaaggagaaa atgaagctct
    23461 taggaatatg aaaaatgaga ttgagctatt ctctttcaat gatttggtct atgtagattc
    23521 agaggacttg aaagctgctc ttggacttga tattgatgat gagggtaact tctatgatcc
    23581 gctttctaaa gaccttgata atgatggtat tgctgatagg tacgacaatg actttagaga
    23641 cagcgattat tttgagtcca cttatgatgt ggaggataac ttaaatacaa aagtggaatc
    23701 tgtaacgaaa gcagaggata agccgtctat tttaagtcag atacgagctt atcaaaatga
    23761 aagtaaaaca gaagaaaaac aaaaaacaaa agaacaggaa attttaagat aaggggaggg
    23821 caacctccct tttgccatga aaggagataa aaacatggat tacaaaacaa tgataaatca
    23881 aatagaagat atggtaaatt ataaccataa ggactttgta aaggcgatta taagcatgga
    23941 aaaaggtatc aatgatgaaa gtgctttaga caagctctat gacgcttata tggacaatga
    24001 cagccttaat ttactgcatg aggaatttga ctatatgatt gaggatttaa gggaacaggg
    24061 acagataaaa gacctgcctt atgttcagga agaaaaggat aatcttatca atattgttgg
    24121 aaatatcgtt ggagaggttg atgtagtaga aagggaaaac aagaatggag aagcctttaa
    24181 ggtggcgaat ttctccgtag tatccaaaga cgatgagggg aataaggtat atcataattg
    24241 ctccgcttat gggggaaaaa gcgatattcc aaaggacttt aagcagggag attttgttaa
    24301 gctctttgga caaataagaa catctattga tgataacggc aaggaacata ggaatatcag
    24361 aatactttct tcaaaactgt taaaggaaaa ggaacaaatg aaaggacaag aagaaaagaa
    24421 agaatctgta cttggagcta ttaagaagta tcaggctgaa gataaggaaa agccaaaaga
    24481 aaagaaagag gcaaaccaag aagctgagag ataaatttat ggtcggtgtg gtcttaggac
    24541 tgcaccgact ctttttttat ttgcacgagt tcgattaaag agaatatatt tatgaaaaaa
    24601 gtatatttta tggtataata tactaaatat agttttcttg agcggaggat aagaatgagg
    24661 gcttttagtt ataaaagatt gttcaaaaaa ctcattgatt tagatatgac aaataatgaa
    24721 ttgatggaaa aggcaaaagt gagtaaaagt acattttata aaatgaaaaa cgggcagaat
    24781 gtaactacgg atgttttgct taggatatgt aacgctttgg attgtgatat tgaggaaatc
    24841 gtggagtgca taagaattga taaatagatt ttgaaaggag gttaagtatg tctaaagaaa
    24901 agtactacat gaacttacca aaagatttat ctggaagcat tgctaaaaat cgctttagat
    24961 tagaattatt atggggaatc agtaagataa tcgatgaaca taaagcgaac aatgaatata
    25021 cgataatttt tgattttaaa tgtgatatag aattgcataa agaggatgaa ttggattttt
    25081 atcaaatcaa gacgaaaaaa agtggtaatt acaattcgaa taatttgtgt aagaaaggta
    25141 agaataagaa caattctatt ttagggaaac tatatgcttt atattcaccg aactacaata
    25201 taaaattagc aattgtgtgt aataaacaac ttaaaatcaa taataaagaa attgattttc
    25261 ctgagcaatg ttttggagat ttagatcagg atgtacttga tgatgttagg aaaaagttgt
    25321 gtacagaatt aaatttagac actgtatgtt tagataatgt gttttatata tttgataaca
    25381 tggatttatt aaatccagag gattctatta gagggaaatt agtaaaatct tttgttgata
    25441 ttaaaggtga agaacctcaa aatccgaatg cattgtatag actggttgtt gattctgtta
    25501 aagaaaaggc ttcttatgaa tttgattccg gtacttatga agatgttgta aaaaacaaag
    25561 gtattacaag aagtgaattt gataaaatgt taaatgcaca taaaaaggaa tctaaaaatg
    25621 gaatcaatga aacacaggag tatataaata acttgtcatt tgctaaacga agaagataca
    25681 atacggcact tggtaatata cttgaaacac agcaaagtga gtctttacga ttaataaaaa
    25741 taaaaattta taattatatt gtggaacatg aaaattcgtt agacgatatt gaaagttatt
    25801 taaaagaaat atcaaaatta tttgatgacg attttgatgt ggaatttact gatgatatga
    25861 aatcggttca atacataatg atttattata tgtatgcttc aggaggaatt ttataatgaa
    25921 tgttatattt gacaaaatat atatatttga tgttgtaaaa aaaaaggctt attcaacaga
    25981 atttaaaaaa ggagttaata ttattacatc gagtgatata gacggaacag atagaggaaa
    26041 atcagtttta ttaagaagtt tataccatgc cttgggtgcg gattctcact ttgactcgaa
    26101 gtggggagaa aaggataagg tctatatctt attttttaag gtggatgata aagatttttc
    26161 gatttataga tctcaaaaat tatttaaaat atttaatgca aaaggtgatc ttatttttac
    26221 tactattcat agaagtgaat tagcagattt ttttggaaat ttatttaatt tttctatatg
    26281 tttgccacaa aaaaatacaa atcaattagt aatagcacca cctgcatata gttttttgct
    26341 gaatttttta gatcaagatg aatacatcgg tacaaaattt aattcgttta aaaaccttgc
    26401 tcagttcagt aattttaaac ttaatgttat ttactcacat ttgggcattt acaataaaga
    26461 atatttcgaa cttgtgaaac acaaagaaga attagaactt gaaattaaaa gtaagaaaga
    26521 agaaatagaa gacctaatta aaatgaaaga cagaaccatt ttaattttgg atggtttttc
    26581 atgtccggaa acaagcagtg cattagaaaa tgagcttaat atagaaactc aaaaatattc
    26641 atcgctaatt aatgagatga atttagtaag gaataagctt gttgacttga gaaatcaatt
    26701 agttgaacag aatatcactt tatctcaaat atctaaattc gaaataaaag aagaaaagga
    26761 aataaaaact attttaaaat caaaagtttg tcctgaatgt cattctgttt tagattcaac
    26821 tttggaacta aggagtaaga gatataatca tattgataat gctcttagtc tgaaagatgc
    26881 aataaaaaca gaaaatgccc atattgaaga tgaaatactc aaaaatgagg gaatatattc
    26941 aaacttaact ttggttttgg aggagcataa taaaaaaatt tgtaaaaaca aaaaggaaat
    27001 caaagactat atcaagttca gaggactaaa taaactaatc gatgagatta ataatgattt
    27061 gactattaat agcagagtta tagacagtac aaaggaggct ctaaagccac taaagaaaga
    27121 atttaaaagt gttaaagata aaattaaatc tgttgatgaa aattactatt atttgattga
    27181 taaattaaaa ataagattca atttaaatga gttagaagct gacagttatg aacaactatc
    27241 taaaaatttt tgtgcaagtg gaagcaacaa accattatca accgttatat ggtatctaac
    27301 actcaatgat ttaaaacaga aatttaatcc tgaaggaaca acatttccaa tggtttttga
    27361 tagccctaac aatgcggaga cagatcaaga gaagaagcag gcattagtcg agtatattat
    27421 ggaatcatca gatcaattta gccagttaat tgtttcggca ataggatttt cagaaaaaga
    27481 ttatagcatt gattcgaata taaatataaa agttttagaa aatgaaaaat atagcttact
    27541 aaatagtgag atgtatgttc agaattatga gcttttacaa tgtatgaacg atgcttaata
    27601 tagcaggggt taattaggag atggctatct ctccatctcc ttatccatat aaattccata
    27661 gttctttcgt atttgataaa gctcgtccat agtagatttt gtagaagaat actcttgcat
    27721 tagggtattc tttttttctt gcaatttatc aagtttatct aaaatgccct ttgaatcagg
    27781 aagctttgaa taggactttt taagttttgc aagagccgtt tcatagcgtg tgatttcagc
    27841 cttatgctcc tcaaaaaatg ccttatcgga tggatttgct ttatattcca tgtagtattg
    27901 cttgtatttt ttaacggtat gaactttttc catagtggta gaaagctcct gcatttcttt
    27961 atcaataatc tttattttat cctgtaaatt ttgtctttcg tcggctactt tttggatgta
    28021 ctcatcaagc tgtttaacgg atttaatgcc atgttctctg atataaatta cagactcagc
    28081 cattgtatta aggttatgtt tggttgccca atattcgtat cctttgcttt cctttacttt
    28141 cacattggta ttcatgtcaa taacattgcc gattcgtttt ttgacaggtg gggttttaat
    28201 cgtttgattt tctgtaatac gctcttttaa tctttcttct gtatagtcct caccaatcgt
    28261 cttagctctt gtaaatctct gtttgtcttt tggcttaaag gctatatgtt ttccatactt
    28321 tatttcataa ccaagctccg ccattttctt tagaaattct tcccaatcct tagactgttt
    28381 tatcagacga tcaatgtcaa attgaagttt gcttttccaa gaagtacctt tcttagattg
    28441 ctcattttca taccatgact taccatttgt tttgtacttt ctcttatagc tctcgtagaa
    28501 cttatcaatc acagaaaggt tattttcttt gcatagctta tcgctttgat acctgatttg
    28561 gtggtagctt ttcttgttgg aatgatagca ttttcctgtt accatattta cattattgaa
    28621 gataatatgg ttgtggatat gccctttatc tatgtgggta gataagacaa attcgtattc
    28681 gtcctttaat atctttttac atagctccat accgatttga tgtgccattt caggacttgc
    28741 ttctccgggt aaaaaggatt gaataaggtg tcttgcaaga acggttccgt ttgttccgac
    28801 atcatttcgt gttcttaaaa attgggtatg agcagtttcc tgatggcatt tatgagtgct
    28861 tactaagatt tgctcatctg ttttttctcc attaacgata tatgaaatgg cgagattaag
    28921 ggttgatttt ataggatgta tttttgtaat agccataaga tttaatcacc tccggaagtt
    28981 ctgttaagga gcagggaatg aatttgccat agctcttttg agaaatgttc aatctcattt
    29041 tttatatcat ttatgtcatc tttatagata acaccagtgg aatttacacg ctttgcaatc
    29101 tgattgatgt tgtttgttgc attggaaagt aagccttgta aatctcggaa aggctctaaa
    29161 tctacctgat aaatttcctt ttccaaaacg catttgcgga taaagtggct catatttctg
    29221 caccttgcga gctttcgttt ctcctcaaaa agagcctttt cttcttcagt tagtttaatt
    29281 tccagtcttt catttcgtat tctatttgcc ataggtaaat cctcctttga atgtatttcg
    29341 gggtcttagg gtatacccta acaagttaaa aattgataaa aaaagaagca tatcccagag
    29401 ggctgcttca tcaatttttt cgtaagtgtc cgtacactta ctgtgcttgc tcatatacta
    29461 atcgtgtaag aacaacttta agcataatac ttatattatt ataccatagt ttggaaaaat
    29521 aaaaaattat atttcatcaa cgtttcataa aaggtgtgta gaatgttatt gaaagtagct
    29581 ataaaatgct attgtgtaaa aaataaagga ggaataattt atgaaaaaaa tagtatcaag
    29641 aggagcatgc ctattacttg cattatgttt gagcttaaac gcagttgggt gtcaaaaaag
    29701 taatgaaaac aataaaatta aagaaggaca atccataagt tctgaagagg caggcatgtc
    29761 aattaatgca aatggcaaaa atgaaacttt taagccatca gattatacat tggaggcaaa
    29821 gaaagaatat gtttatgaat atttagggct taaatttaaa ctctcagaca aatttagaaa
    29881 ttatatagct gataaaaaga tagcgatgtt agatgatcaa agtcctatag ataaagaact
    29941 aaagtatgct attttgacat ttgaaaaaat gacagaagaa caaaagaatg cagttataga
    30001 aaagatgggc gatggatata aaaattggca aaatgagctt gaaagaattg ggactattgg
    30061 tatattcgaa aaaaatacat cagaagaaaa aatatctaaa attacaaaat gtgatactca
    30121 tactaaaata ggagtttcaa gtgatggaaa atacgattgc tatttaagca caaatagcgg
    30181 agctgaaagt aatcttctgg atgaattaaa aagaactgag attcaaatta tagataaaaa
    30241 agaacgtcct aaaaatggtt ttgtgctatc agaaaaaact gatttagaaa atacagaagc
    30301 atttaacaaa gaatcggtta aagatttacg taaactatca acaaaggaca taaatggcaa
    30361 ggattttaca agtaaagatt ttgagaagta tgatcttact atggtaaatg tatttgcaac
    30421 ttggtgtaca gcttgtgtaa aggaaattcc tgatttggtt gaggttcaga atgaaatgaa
    30481 aagcaagggt gtaaatatcg ttggagttgt tacagatact gttgatgata acggtgaaaa
    30541 caaggaagca attgaaaagt caaagttaat acatgaaaag acaaaggctt catatccttt
    30601 cttaatgcca gataagacaa actttaatgg tagattgaat ggtatacagg ctatgccaga
    30661 gactttcttt gttgatagta atggaaatat tgtaggtgat acttattctg gggcaaagag
    30721 tgcaaaggaa tggaagcagg taattgaaaa agaattggaa aaaataaaaa ataaataaaa
    30781 aataaataaa agtaaagcgg tgattggtat atggttaata agtttttaaa aagccgtgcc
    30841 ttgtctttat taattatctt tattagtatg gcgatgattt actatggtca tagtaatgga
    30901 gaaactaaag ttgtcttgga taaggcgata agaatttgta tggagtgtat tggaattgga
    30961 tagaattaaa aatattataa atggtagtac aaaaaaagat aaatataaaa taagagatcg
    31021 ttttaggcat ttacttcaat ccgtttggtt tttaattact aattcttact ttgaagggtt
    31081 taagactggt aaaatctacg gtgggaattt aaagaaaatt tgtgttccag gtatgaattg
    31141 ttattcatgc ccgggagcaa aaggttcttg tccaatagga tcactacagg cagtaattgg
    31201 aaattcaaaa tataagatta gttattatat agttgggatt ttgtttttta taggagcatt
    31261 ggtgggaaga tttgtttgtg gatggctgtg tccatttgga ttgattcaag atttgctaca
    31321 caagattccc ttttttaaaa aagttgaaac atttaagttt gataaacatt tgagaaaatt
    31381 gaaatacata atattacttg tatttgtaat aatattacct ttatttttgg ttgatatttt
    31441 aggtcagggt tctccgtatt tttgtaaact tatttgtccg attggtatgc tggaaggagg
    31501 attaccactt gttttattaa ataaaagtat gagaagtgcg ataggtttct tgtattattg
    31561 gaaaggaatc attctaatag ttactttgtt actttctata attatgtata gacctttttg
    31621 taaatatata tgtccacttg gagctattta ctcaattttt aaccctattt caatatttag
    31681 atatagattg gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat
    31741 gaatatagat ccagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa
    31801 aaatgcttgt ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag
    31861 gaaagtgata aaaaatgcta tagcatatat tacaagaaag agaaatagaa ctttaataat
    31921 ttttatcata ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc
    31981 aagcaatgaa atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa
    32041 aaaagatggt aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat
    32101 tgaagaaaaa ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag
    32161 cggagaacaa agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact
    32221 tgaagctaca aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa
    32281 agaaggtaaa aatataggag aaaatgataa gaattcaatt attgttcatg aagaatttgc
    32341 taaacaaaac aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa
    32401 aagtggaaaa ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca
    32461 ggaaatatat acaggattat cttctgattt tagtgaaaat atcgtgtttg tagattattc
    32521 aacaagccaa gagatattaa ataaatcaga aaataataaa atcgcaaata aaattttaat
    32581 gtattctggc agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat
    32641 tgatgagtca aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc
    32701 agtgagtgga ataaaacata taataaaaat aatgacttat tctattatgt taggtgggat
    32761 ggttgttctt tcattaatct tgattctatg gttaagagaa agaatttatg aaataggtat
    32821 atttttatct attggaacat ctaagataca aattataatg caatttatat tcgagttaat
    32881 attcatatcg ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt
    32941 aattgtagat ggattcatta actcagagga ctcaatgatt tccggtggaa gtttaataaa
    33001 taatagtagt tttatgttaa atataacaac acttggacaa agttatttaa tattaataag
    33061 tattattgtt ttatcagttg tatttgcttc ttcattaata ttaatcaaga agcctaaaga
    33121 aatattatca aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc
    33181 atatagttat gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact
    33241 tgggaagttt tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt
    33301 acttgctgga cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca
    33361 aaagaaagga tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa
    33421 tttaatagat tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga
    33481 aagtatcttg tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt
    33541 atctggtgga cagcaacaaa gggtagctat tgctagagca ttggtatcag atgctccaat
    33601 aatactagct gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa
    33661 tatattaaag acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa
    33721 ggaagtagca gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt
    33781 aaataaaatg aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga
    33841 aaaagcttaa aatcattaat tattatatta gttattttat ctatgtcaac tttaagtctc
    33901 attagtttat ctattaaaga tgctacggac agagcttcaa aagaaacatt tgataatata
    33961 acaaatagtt tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg
    34021 ggaaatgtaa aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat
    34081 gtaaaaagaa taaacagtgt tgcagattta gttgattatg atattattga aactcaagag
    34141 actcttgcga atcaatcacc tgaaagagcg aagaatttta aaagaacagt tatgttaact
    34201 ggggttaacg actcatcaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa
    34261 ggaaagcatt tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa
    34321 aaaaataatc ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat
    34381 aatgagaaag gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat
    34441 aatagcggtg gagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatgtt
    34501 catacagctg ctaaagttta tggtaataca gaagatacag ctgtatacca agatgcaaca
    34561 ttctttgtaa aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat
    34621 ataaattgga gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa
    34681 tctatatcag gtatctactc aatttcaaat aaattatttg ttggctcatt aatatttgca
    34741 ggagttgtag tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca
    34801 gtattactat cattaggtat atcaaaatta gaaatctttg gtcaattcct aattgagatg
    34861 gtatttatat caatccccgc attcgttggt tcttatttct tagctcaata tacagctgat
    34921 aagcttggga ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaga
    34981 caatctgcat ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta
    35041 tcaagtttag atataaatgt attacctaaa ttcataattt atgtagttat atttatgagt
    35101 ttagtacttc ttgtatcatt gattatttcc tcattcaata tattaagaag gaatccaaaa
    35161 gaattattaa ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aattttttgg
    35221 cttttaaata tcaaataata tgctgtataa tataagtgta taggcttaag tggaggtata
    35281 tatgaaaata ttaactgttg aagatgataa tatgataaga gagggaataa gtgaatatct
    35341 ttcagaattt ggatatactg ttattcaagc taaagatgga agagaagcct tgtcaaaatt
    35401 taatagcgat ataaatttgg ttatcttaga cattcagata ccttttataa atggtttaga
    35461 agtgttgaaa gaaattagaa agaaaagcaa tttaccaatt ctaatcttga ctgcatttag
    35521 tgatgaagaa tataaaattg atgcatttat taatttagta gacggatatg tagaaaagcc
    35581 attttcattg ccggtcttaa aggctagaat agattcttta attaagaaga attatggaca
    35641 tttagagaag tttgaatata aggatctatc ggttaatttt aatagctata cagctaaaat
    35701 aaatggtgaa gaaatagatg taaatgcaaa agaactagaa gtattaaaat gtttattgga
    35761 taataatgga caagtgttaa caagaatgca aattattgat tatgtatgga aagatagcga
    35821 agaaactcct tatgatcgag taatagatgt atatataaaa gaacttagaa aaaaattaca
    35881 attagactgt ataactacta taagaaatgt aggatataaa ttggagagaa aatgaaaaaa
    35941 ttaaagatat ttccaaaaat gttcatacaa atattttctg ttcttggaat aataattata
    36001 ttagttcatt cattagtttt ttttatcttt cctaaaacat atttggagac gagaaaagaa
    36061 aagattcata atatggcaaa tgaaatttct agtaatatga atggaaaaga aataaaatat
    36121 atagaacaaa ctttggagct ttattctaag agtagtgaaa taaaagcatt tataaaagaa
    36181 aaaaataata agaatgaaat acaaatcaaa gataatataa atgttaattt agaaagtgat
    36241 agtaattctt tgataattga agaaagagaa ataaagctta atgatggtaa aaaaacaaat
    36301 ctacaatttg tttctacagc tgatatgcaa aaagatgcga aagatttaag ccttaaattt
    36361 ttaccatatt ctttattaat atcaatttta ttttctgcta ttatttcttt aatttatgca
    36421 aaatcaataa aaaataatat acaagaaata aaaattgtta ctgataaaat gatgaaactt
    36481 gataaaaaaa cgcgtttaaa agttagctct aatgatgaag ttggagaatt aaaacaacaa
    36541 attaatgatt tgtattctac tttattaaga acaattgacg atttagaatt taaaaataaa
    36601 gaaattttaa aattagaaaa gttaaaatat gactttttta aaggtgcatc ccatgaactt
    36661 aaaactcctc ttgctagtct taaaataata ctggaaaaca tgaaatacaa tattggaaaa
    36721 tataaagaaa gagatattta tattaatgaa tgtattgaaa tcgttgatag tttaacaaaa
    36781 aatatttctc aaatattatc agttcattct atagaaaatt tgaataatga tgaagaatat
    36841 ttgaaaataa atgatacatt agaagacata ttaaaaaagt atgaaatatt agcaaatcaa
    36901 aagaaaataa ctgtcaataa ttatatatta gatgagaatg tttatatagg aaaaacagct
    36961 ttaaagatta ttctatctaa tttgattagt aatgcggtaa
//
LOCUS       DAEMIK010000021        36566 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEMIK010000021 DAEMIK010000000
VERSION     DAEMIK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.021"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..341
                     /locus_tag="KPK87_000898"
     CDS             <1..341
                     /locus_tag="KPK87_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2537072.1"
                     /translation="VKLDTDGYMRILNNLIQNVISHSHADKIEIILSKQEKNMQIRLA
                     DNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQLVEKMNGTITANSIQGKGT
                     EFTLLFPLAK"
     gene            478..1404
                     /locus_tag="KPK87_000899"
     CDS             478..1404
                     /locus_tag="KPK87_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537073.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGVPNRHAIKDALDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGTLLEEES
                     LAELEQKSSKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            1397..2182
                     /locus_tag="KPK87_000900"
     CDS             1397..2182
                     /locus_tag="KPK87_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537074.1"
                     /translation="MLKLIKCEFYKFKRRSLFVLATLVALVFPVVMTALYWNIPTQVN
                     YDNLFSGIVDYGDFLLLLPILVVIATSLFFTETDNDTLKNLTTIPISKSKIVVAKIGV
                     MAIISVAYTLLGFFASVLCSKILGIAMENMAQKFVLSIGLGIMLLAAALPCVALVVWF
                     NKSDLISIVITLFYTIINYVIHVTDIGMLTPVGVNIGTILPIPVINRWIYQFYDEGNG
                     AAAEFYNTMRPYFVPTHICVCIIALFLFVSIYAIIKIYNRREI"
     gene            2194..3012
                     /locus_tag="KPK87_000901"
     CDS             2194..3012
                     /locus_tag="KPK87_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537075.1"
                     /translation="MLAFITTEFQKLKRYSIILVGMLGVACSPIISIVMQKIMTDEAK
                     NVLNYTFPDLLNSTIWNNMTMFFPMIIALIGGYMINREYTDDTLKNLQVIPISFPKLM
                     IGKLTTIGLLSILLGLCNSFVTIIVGIIACRENMTLSTIIIGTIQICGMALFTFIGEM
                     PLIAHCGKKQNSFKGGAVAAFLLGYISIYFKNPIIRNLYPLSAGLSIVGFNGEGFVTD
                     TTGAYSSSQSILLGVLTMLAMLILSLIIISIPQKETLATPLSQKRRKTERKIAK"
     gene            3009..3467
                     /locus_tag="KPK87_000902"
     CDS             3009..3467
                     /locus_tag="KPK87_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537076.1"
                     /translation="MKKLIPLFLMLCCLVSVTACSKDEIVSNYNQALQSIGDKGLTDD
                     KDLQGKREFGIDSYVGNYTADYDDFSGNEIIFGGTALERDNGNKIEISCDIAAQTGNL
                     KLILQTGTDEPKILCDTESSYNDTIELPSASNYVLIEADNFTGSLKLKLK"
     gene            3487..3750
                     /locus_tag="KPK87_000903"
     CDS             3487..3750
                     /locus_tag="KPK87_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537077.1"
                     /translation="MKEKILSIISLVTIFVPLTMLFVWKPTAANATAIAIGYGVFIVA
                     SFLYALFLFLKKQQRDIYVKVGLGVNAFYLLGILFMVIIPRLF"
     gene            4207..4647
                     /locus_tag="KPK87_000904"
     CDS             4207..4647
                     /locus_tag="KPK87_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2537078.1"
                     /translation="MTPNRREFEKQCAFQKFCKSVLHNEACNAHEEIRRRRVREVTFS
                     DLALHEERQLYTVDKYFQDEEAEPSYQMAGKEITPKLLLEAIRALPEERRKIVLLYYF
                     EGLTDIEIGKQLNISRSTVQYRRTSSFEQLKKYLEENADEWIEW"
     gene            4628..4885
                     /locus_tag="KPK87_000905"
     CDS             4628..4885
                     /locus_tag="KPK87_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2537079.1"
                     /translation="MNGLNGKPTEYPENTLVPYPVILAASKGDPDAMKIVLQHFSGYI
                     ARLSMRKLYDERGNVYFGVDEEIRERLQTKLMRAILTFKAE"
     gene            5304..5507
                     /locus_tag="KPK87_000906"
     CDS             5304..5507
                     /locus_tag="KPK87_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HBG2537080.1"
                     /translation="MNNNDVPIWEKYTLTIEEAAKYFRIGESKLRKLAEENPVPDWVM
                     MNGNRIQIKRKQFEKMIDTVDAI"
     gene            5587..6780
                     /locus_tag="KPK87_000907"
     CDS             5587..6780
                     /locus_tag="KPK87_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2537081.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLGKQLINVDHQLLKIADVGYYVETPKTKSGNRVIPMSE
                     KVLEAFQRVLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
     gene            7061..7909
                     /locus_tag="KPK87_000908"
     CDS             7061..7909
                     /locus_tag="KPK87_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537082.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMSNNINSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNISNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(8346..9434)
                     /locus_tag="KPK87_000909"
     CDS             complement(8346..9434)
                     /locus_tag="KPK87_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537083.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            9559..10287
                     /locus_tag="KPK87_000910"
     CDS             9559..10287
                     /locus_tag="KPK87_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG2537084.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(10489..10980)
                     /locus_tag="KPK87_000911"
     CDS             complement(10489..10980)
                     /locus_tag="KPK87_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537085.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(10991..11434)
                     /locus_tag="KPK87_000912"
     CDS             complement(10991..11434)
                     /locus_tag="KPK87_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537086.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(11424..12263)
                     /locus_tag="KPK87_000913"
     CDS             complement(11424..12263)
                     /locus_tag="KPK87_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537087.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            13033..13479
                     /locus_tag="KPK87_000914"
     CDS             13033..13479
                     /locus_tag="KPK87_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2537088.1"
                     /translation="MIKFEKLNIINNKDMKEILDTWESSVRATHDFLSEEKIISIKPQ
                     VKEGAKYVSNFLCVRDKKGLIKAFMGIHDFKIEMLFVGNYSIGNGIGKRLAEYAIEVL
                     NVNYVDVNEQNQQALGFYKHMGFKVFKKSEFDEQGNPFPILHMKLK"
     gene            complement(13709..13849)
                     /locus_tag="KPK87_000915"
     CDS             complement(13709..13849)
                     /locus_tag="KPK87_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537089.1"
                     /translation="MKNENISYLALGLCFGVILGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(14410..15795)
                     /locus_tag="KPK87_000916"
     CDS             complement(14410..15795)
                     /locus_tag="KPK87_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2537090.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDGLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDSCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTSMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKY
                     TVGSFEYTPII"
     gene            complement(15968..16711)
                     /locus_tag="KPK87_000917"
     CDS             complement(15968..16711)
                     /locus_tag="KPK87_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG2537091.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(16731..17993)
                     /locus_tag="KPK87_000918"
     CDS             complement(16731..17993)
                     /locus_tag="KPK87_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2537092.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(18177..19175)
                     /locus_tag="KPK87_000919"
     CDS             complement(18177..19175)
                     /locus_tag="KPK87_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG2537093.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(19159..20670)
                     /locus_tag="KPK87_000920"
     CDS             complement(19159..20670)
                     /locus_tag="KPK87_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG2537094.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(20724..21686)
                     /locus_tag="KPK87_000921"
     CDS             complement(20724..21686)
                     /locus_tag="KPK87_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG2537095.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(21734..22624)
                     /gene="rbsK"
                     /locus_tag="KPK87_000922"
     CDS             complement(21734..22624)
                     /gene="rbsK"
                     /locus_tag="KPK87_000922"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG2537096.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(22666..23679)
                     /locus_tag="KPK87_000923"
     CDS             complement(22666..23679)
                     /locus_tag="KPK87_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2537097.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(23908..24885)
                     /gene="bioB"
                     /locus_tag="KPK87_000924"
     CDS             complement(23908..24885)
                     /gene="bioB"
                     /locus_tag="KPK87_000924"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG2537098.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(25091..25354)
                     /locus_tag="KPK87_000925"
     CDS             complement(25091..25354)
                     /locus_tag="KPK87_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537099.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(25424..26497)
                     /locus_tag="KPK87_000926"
     CDS             complement(25424..26497)
                     /locus_tag="KPK87_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG2537100.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(26745..27503)
                     /locus_tag="KPK87_000927"
     CDS             complement(26745..27503)
                     /locus_tag="KPK87_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537101.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(27500..28417)
                     /locus_tag="KPK87_000928"
     CDS             complement(27500..28417)
                     /locus_tag="KPK87_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537102.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(28417..28617)
                     /locus_tag="KPK87_000929"
     CDS             complement(28417..28617)
                     /locus_tag="KPK87_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG2537103.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(28622..29716)
                     /locus_tag="KPK87_000930"
     CDS             complement(28622..29716)
                     /locus_tag="KPK87_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537104.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(30226..30366)
                     /locus_tag="KPK87_000931"
     CDS             complement(30226..30366)
                     /locus_tag="KPK87_000931"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG2537105.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(30676..31983)
                     /locus_tag="KPK87_000932"
     CDS             complement(30676..31983)
                     /locus_tag="KPK87_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2537106.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(32048..32527)
                     /locus_tag="KPK87_000933"
     CDS             complement(32048..32527)
                     /locus_tag="KPK87_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG2537107.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(32574..33437)
                     /locus_tag="KPK87_000934"
     CDS             complement(32574..33437)
                     /locus_tag="KPK87_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2537108.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGALF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(33437..34192)
                     /locus_tag="KPK87_000935"
     CDS             complement(33437..34192)
                     /locus_tag="KPK87_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2537109.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(34219..34707)
                     /locus_tag="KPK87_000936"
     CDS             complement(34219..34707)
                     /locus_tag="KPK87_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2537110.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(34725..35126)
                     /locus_tag="KPK87_000937"
     CDS             complement(34725..35126)
                     /locus_tag="KPK87_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG2537111.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(35129..35626)
                     /locus_tag="KPK87_000938"
     CDS             complement(35129..35626)
                     /locus_tag="KPK87_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2537112.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQTDDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(35650..36054)
                     /locus_tag="KPK87_000939"
     CDS             complement(35650..36054)
                     /locus_tag="KPK87_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2537113.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(36284..>36566)
                     /locus_tag="KPK87_000940"
     CDS             complement(36284..>36566)
                     /locus_tag="KPK87_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2537114.1"
                     /translation="NRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESLVYKSYEER
                     ISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
BASE COUNT        11897 a         6622 c         4530 g        13517 t
ORIGIN      
        1 gggtaaaact tgatacggac ggctatatgc ggatattgaa caatcttatt caaaatgtaa
       61 tttctcacag tcatgcggat aaaatagaaa taatcctgtc aaagcaggaa aaaaatatgc
      121 aaatccgatt agcagataat ggaataggga ttgaaaaaga agatttgaag catatttttg
      181 aacggctcta taaatgcgat aagggacggt ctgaaaaagg cagcggtctt gggctttcta
      241 tcgcacacca acttgtagaa aagatgaacg gcacaataac agcaaatagc atacagggaa
      301 aaggaacaga atttactttg ctttttccct tagcaaagta aggttctccc gtccttttga
      361 cgggagcctt aattatttgg ggagaaatat ttcaaattgc aaggttaatg caaggttgcg
      421 gcaaggttaa tgcaaggttg gtgtgataga atactttaca gaacaggagg tttgaatatg
      481 gacaaaaatt atatcattga aacaaaaaat cttacaaaac agtacggctc acaaaagagt
      541 gttgctgact taaatattca tgtgcagaaa gggagaattt acggactgct tggaagaaac
      601 ggagccggaa aaacgaccac tatgaaaatg ctacttggat tgacaaagcc aacttccggc
      661 gaagttaaaa tatggggaaa gtctttgcag gggaatgaaa agaaattgtt gccccgtatc
      721 ggtagtctga ttgagtcgcc cggtttttac cctaatttga ctgctacgga aaacttacaa
      781 atttttgcta ccttacgggg agtaccaaac cgccacgcaa tcaaagacgc tttagatttg
      841 gtgggattgc cttataaaga caaaaaactg ttttcccagt attctcttgg tatgaaacaa
      901 cggcttgcga ttgcacttgc tgttatgcac gacccggagc ttttgatttt agatgaacct
      961 atcaatgggc ttgacccgat tggaattgca gaggttcgtt cttttattcg cgagctttgc
     1021 gatacaaagg gaaaaacaat tttaatttcc agtcatatcc tttcggagat ttctctgcta
     1081 gcagatgata ttggaattat cgaccatggc acactattgg aagaagaaag tcttgcggaa
     1141 ctggaacaaa aaagcagcaa acatatccga tttactcttt ctgataccgc acaggcagca
     1201 agaattttag agcgtacttt ccatgagagc catttctcta tacaggacga ccataattta
     1261 cgcttgcata atcttgattt gtccgtagga aaaatcgtga ctgcttttgt agaaaacgga
     1321 ttggaggtat cagaagctca tacttgtgaa gaaagccttg aagattattt caagcgtgtg
     1381 acaggaggcg agggaattgc ttaaactgat taaatgcgaa ttttacaaat ttaaacgacg
     1441 ttctcttttt gttttggcta ctcttgttgc tttggttttt ccggtagtaa tgactgcttt
     1501 atattggaac attccaacac aagttaatta tgataatttg ttttccggca ttgtggacta
     1561 tggcgatttt cttcttttgt tgccgatatt ggttgttatt gcaacttctc tgttctttac
     1621 agaaacagat aatgacactt taaagaacct tacaaccatt cctattagca aaagtaaaat
     1681 agttgtagca aaaataggag taatggctat tatttcagta gcttatactc ttttaggctt
     1741 ttttgccagt gtgttgtgtt caaaaatcct tggtattgct atggaaaaca tggcacaaaa
     1801 atttgtattg agtataggat tgggaataat gctcctagct gcggctttac cttgtgtggc
     1861 attagttgta tggttcaata aaagtgactt gatttctatt gtcattaccc ttttttacac
     1921 aattattaac tatgtaattc atgtaacaga tataggaatg ctgacaccag taggggtaaa
     1981 tataggaaca atcttaccta tacctgtaat caatcgttgg atttatcaat tttacgacga
     2041 ggggaatgga gccgcagctg aattttataa cactatgcgt ccttattttg tcccaactca
     2101 tatatgcgtc tgtattattg cgctctttct gttcgtatca atttatgcga ttataaaaat
     2161 ttataatcgt cgtgagatat aggaggtgtg aatatgcttg cttttattac aacagaattt
     2221 caaaagctaa aaagatacag tattattttg gttggtatgc ttggtgtagc gtgttcccct
     2281 attatttcaa tcgttatgca aaaaattatg acagatgaag ctaaaaatgt cttgaattat
     2341 acatttcctg atttacttaa tagtacgatt tggaataata tgaccatgtt ttttcctatg
     2401 attatagctt tgattggtgg ctatatgatt aaccgagaat acactgatga tacactaaaa
     2461 aatctgcaag ttattcctat ttcttttcct aaactgatga ttgggaagct aactacaata
     2521 ggactacttt ctatactttt aggattatgt aattcatttg tcactattat agtcggaata
     2581 atcgcttgtc gtgagaatat gactttatca acaattataa ttgggactat acaaatttgt
     2641 ggaatggcat tatttacatt tattggagaa atgccactta tcgcacactg tggaaaaaaa
     2701 caaaactctt tcaaaggtgg ggctgttgca gcttttctat taggatatat tagtatctac
     2761 ttcaaaaatc ctattatcag aaatttgtat ccgctctctg ccggattaag tatagtcggc
     2821 tttaacggag aaggatttgt gacagatact accggagctt attcatcttc tcaaagcatt
     2881 ttattaggtg ttttaacgat gttagctatg cttatattat cattgataat aatttctatt
     2941 ccacaaaaag aaactttagc taccccttta tctcaaaaaa ggagaaaaac agaaaggaag
     3001 attgcaaaat gaaaaagctg atacctttat ttttaatgtt atgttgtctt gtttctgtaa
     3061 ctgcctgttc taaagatgaa attgtaagta attataatca agccttacaa tctattggag
     3121 ataagggttt aacagatgat aaagatttgc aaggaaaacg tgaatttggt atagatagct
     3181 atgtaggaaa ttataccgct gactatgatg atttttccgg taatgaaatc attttcgggg
     3241 gaacagctct tgaacgagat aatggaaata aaatagaaat ttcttgtgat atagctgctc
     3301 aaaccgggaa tttgaaacta attttacaaa cgggaactga tgaaccgaaa atcctttgtg
     3361 atacagaaag ctcatataat gatactattg aacttccgtc tgcaagcaat tatgttttga
     3421 ttgaagcaga taattttaca ggttcactga aattaaaact caaatgataa tagaaaggaa
     3481 accattatga aagaaaaaat tttaagtatt atatcccttg ttacaatttt tgtacctctt
     3541 actatgttat ttgtttggaa gcctacggct gcaaatgcaa cagctattgc tattggttac
     3601 ggtgttttta ttgttgccag ttttctttac gcactattct tatttctcaa aaaacagcaa
     3661 cgcgatattt atgtaaaagt tggattgggt gtaaatgcct tttatctact tgggatatta
     3721 tttatggtta taatcccacg tcttttctaa aatttaaaat ctaaatatct gccggacgac
     3781 ggcaaaagaa aaaaagccgt cgcagagcag atacattttc acgcgccttt gaagcggtgg
     3841 ctttgatttt taaagccgcc gcttcttttt gtctactgct acctataaca taaattagcg
     3901 gcatttagga gggctgccgc tatgggaatt tctttgctgt tatacagcat acataggttg
     3961 cattcattaa aaaaagtaca gttccaccac cgggtaagtc tggttataga tatgaactgt
     4021 caaatcaatt aaataccgct gttatttcct atgcgcctat ctgcgttttg caggcaggcg
     4081 cattttgcat tttacagaag atttttcaaa aactttgctc tctttttggc aaagcaaaaa
     4141 gtcgcctgta ttatcccgcg aaaaggggag gtactaccgc ctttcggcag aaaggaggtg
     4201 aacaccatga cacctaatcg cagggaattt gaaaagcaat gtgcgtttca aaagttctgt
     4261 aaatcagtgc tgcacaatga agcgtgcaac gcccatgaag aaattcgtcg gcgcagagtt
     4321 cgggaggtta ctttttctga ccttgccttg catgaggaac ggcagcttta taccgtcgat
     4381 aagtattttc aagacgaaga agcagagccg tcttatcaaa tggcaggaaa agaaataacc
     4441 ccaaaactgc tgcttgaagc tatccgggct ttacccgaag aacggcgcaa aattgtactg
     4501 ctgtattact ttgagggatt aactgatata gaaatcggaa agcagctcaa tatctcaaga
     4561 agtacggtac agtatcgccg gacaagctcc tttgagcagt taaaaaaata tttggaggaa
     4621 aacgctgatg aatggattga atggtaaacc gaccgagtac ccggagaaca cccttgtacc
     4681 ttatcctgtc atactggcag cgtcaaaggg cgacccggac gcaatgaaga ttgtattgca
     4741 acatttcagc ggctacatag cccgtctttc catgcgaaag ctctatgatg agcgaggaaa
     4801 tgtttatttt ggagtagacg aagaaatacg cgaaaggctg caaacaaaac tgatgagggc
     4861 aatcctcaca tttaaggctg aataaaccaa cgcggtattg gaacgcttcc cccttttctg
     4921 ttccatatcg cagaacagcc ctctgctctt tgataaagaa agcggcgcaa tagaacggcg
     4981 acgcagtata aggcaaatcg gatacgttcc ttttgtgccg agccatgcgg tggatacgcc
     5041 atgacctttc tcttaataga aaagcgagcg agaaatacca caccgtaaag cagagatagc
     5101 agcctgcggg cgataacgca cagaatacat aatgatactt ccttatagcc atagtccgag
     5161 cgttagaagc gtcgcaggca atgggtaggg cttcggcaga ccgccggagg ggtgcaagtc
     5221 ccatgaggtt acgctaatag ccgtccgatt aaagcctatg ttacagattg atttttgacc
     5281 cattaagaaa gggggacgac aaattgaaca ataacgacgt gccgatttgg gaaaagtaca
     5341 cgcttaccat agaagaagca gcaaaatatt tccgtatcgg agaaagcaag ctgcggaaac
     5401 tggcagaaga aaatcccgtc ccggactggg taatgatgaa cggaaaccgt attcagatta
     5461 agcgtaagca atttgaaaaa atgattgata cggtggacgc aatctgatag tgaaatcgag
     5521 ccttgaatgt ggtacaatat agtcaagcat atcaaggctc tttccattat ggaaaggagc
     5581 ataacgatgt cggaaaaaag acgggataat aaaaatcgta ttctccgcac cggagagagc
     5641 cagagaaaag acgggagata tgcctacaaa tatatagata cctttgggaa accgcagttt
     5701 gtgtattctt ggaagttagt gcctacggac aaaaccccgg caggaaagcg cgacgatata
     5761 gcgttgaggg aaaaagagaa agaaatccaa aaagaccttg acgacggtat cgaccatatc
     5821 ggaaagaaaa tgacggtctg ccaactctac gcaaagcaga tacgccaccg ggcaaatgtg
     5881 cggcatggta caaagcaggg gcgaaaacag ctcatgcgga ttttgcagga ggataaactt
     5941 ggagcctgcc ggattgaaaa tgtgaagctc tccgacgcaa aggaatgggc gttacgcatg
     6001 aaagaaaaag gttacggctt caagactatc aacaaccaca agcgttcctt aaaggctgcc
     6061 ttttacacag ccatacagga cgattgtatt cgtaaaaatc catttgactt ccagttgaac
     6121 acagtccttg aagatgatac ggaaccaaag gaacctctat cccctacgca ggaagcggct
     6181 tttctgtcct ttgtgcagca tgataaagtc tatcagaaat actacgacga gattatcata
     6241 ctgttaggga cagggcttcg catttcggaa ctctgcggac tgacggaagc tgacattgac
     6301 ttaggcaaac agcttatcaa tgtagaccac caacttttga agattgcaga cgtgggctac
     6361 tatgtggaaa cgcctaagac gaaaagcggc aaccgggtta ttcctatgag tgaaaaagtg
     6421 ttagaagcat ttcagcgggt gctgaacaag cggaaatatg cacagcctgt tattttagaa
     6481 ggctacacaa agtttctgtt ccttaaccgg aacggtttgc caaaagtggc agtcaactat
     6541 gaaagtatgt tccgggggct tgtaaggaaa tacaacaaaa cgcaaaaggt agcattgcca
     6601 aaggtaatga caccgcacac cttacgccac acattctgca ccacgttagc aaatgcagga
     6661 atgaacccaa aggcattgca gtacatcatg ggacattcca acatcaatat gacgctgaac
     6721 tattacgcgc atacgacttc cgaaacgtcg ataacagaaa tgaagcggct gattgcctaa
     6781 gtagtaaacg gagaatttac tactccacat actacttttg aacaccaaaa catacgggga
     6841 tataagaaga tttgagagta tcttccacaa tgaaaaatgc cggaaaagcc tgtaacaata
     6901 ggctataccg gcatataaga acttttgaaa agataatcaa aaattacttt gtgatttaat
     6961 aaaaatagaa tgcacatatt ataaactttt ttaatatata tattattaga ataagttttt
     7021 gtaaactttt ttatagtttt ctaggaggtg caagtttaat atgagtaaca agaaaaaaaa
     7081 ggatttagat actagttaca tgccttactt agacccagcg aactatgcag attatattac
     7141 taatgaagaa attccccaag atgaattgag aaatgaccct caaatttcta atggttatac
     7201 aaaaatacct aataaccaga acataaataa cttatatcct aataatgtaa atcattatcc
     7261 taaaagtagt aattctccaa acaacttgcc taacacaaat aatacatccg gaaatatgaa
     7321 ttacaataca tctgctacaa ataatatgcc taataacatg aataataata cgggtatgcc
     7381 taataatatg tctaacaata tgaatagtaa tatgggtatg cctaacaata tgtctaataa
     7441 cattaatagt aatatgggta tgcctaacaa tatgcctaat aatatgaata gtaacatggg
     7501 catgcctaac aatgttccta ataacatgaa tagtaatatg ggtatgccta acaatatatc
     7561 taataatatg aatagtaata tgggtatgcc taacaatatg tctggcaata tgaataacaa
     7621 tatgtatggt aatacaggta tgcctaataa catgaacagt aatatggggt ataatatgaa
     7681 caatactatg ggtattccta acttctcatg taatcaaaat atgcctccta atgtacttat
     7741 gatgatacca ggagttatat gtcataatac aatgcaaggt atgcctgtag ttatgcctag
     7801 tactatgcct cctaatatat atcctactcc ttatggttct agtaatatgt ctatacaagg
     7861 tataccacag gctactaata ttgaagaatt tgatgaggaa gaaatgtaat atatattttt
     7921 ttaagtaagt tgtataaaac attaataatt gaccataata ataaatgtat tcctcataat
     7981 attcccccaa tattattata ggtatagaaa gttgactgaa tatgcgctta ttcggtcaac
     8041 agatattaac ataatctaat aagataaaca tagaaaaagt cagataaaat ttaattttat
     8101 ctgacttttt catttaaaaa tgctctcttg tgttggtgat tcagaatagt atgatactcg
     8161 tatttgagac taatattggc aaacaacata aagaaaaata taaatttttt ataataaact
     8221 gtataaaatt ttaataattg accacaataa taaatgtatt cctcataata ttcccccaat
     8281 attatagaaa taaatttaag cgagattaaa ttcagttaat ctcgcttaaa taatgtctaa
     8341 atttattatt ccaaaaaatc atcaaaatct aatgaacctg attctgcaaa atcatataat
     8401 gcattttgct caaagaaaaa tttctcttca aatttaatat agtcgctata attagattga
     8461 cttatttcat tgatataatt gcaatcattt tcgttataag taccaataaa aaacttattt
     8521 cccattaagt aatatatagc tttcatatca ttatttatta tatatacatt ttcttttaca
     8581 tggtcattat taaaattaaa tctagtaaag aatatacctt cattcatatt actttttaac
     8641 agaaatggat tttctctata aaatactcct gcaaattctt caggtgaatt tacactgtaa
     8701 acacttacaa attcactttt agaaatctca tcaaatacct caaaatcata cataggttct
     8761 ttatctgtca ctaatagaga attaattcta aaaccagttt cataacactt acttatgcaa
     8821 aaagcaattt tacatatata atatccatca ttatcttcat ataaagcttc acttatatat
     8881 ctgccttgac ctttatcact taccacgttt ttaagtaatg ttccatttat attagtaatg
     8941 tgcatatttg caatctcgtc acttccagaa aagtacttta aactttctct atcccatgct
     9001 ataaatctca tggtcatata gtttataaat tcaacttcct cgtcaactct tttacatatt
     9061 ttattgtata atttttctat agttaaatct gttttatagt tttttataat atcaatatac
     9121 tcacatttat ctcctggcaa tagcttatca taataaagat tcttattacc aaaataagat
     9181 actaaaaata aagattcatc cttcgttaat tcaactctat ctgaaccaaa tcctcccatc
     9241 aatctttctt cttccatata agcttcttct tgtgttgggt tatttaaact cacataatcc
     9301 gctaatccta atccctctgc atctaataaa aaatattgac aaaagtgatt gttttcatca
     9361 tctatccaat ttattataag acctaaactg cccataagtc tagaatttgt agcctttgca
     9421 aaattaaaat tcatatagtc acatccttta aactctaaaa tacttgtttt ttaatatata
     9481 aattattata catatatttt aacaacttag atacaattta taatataata taaggaaatg
     9541 attaattgag gtggtcttat gaaaaatata gcagcttttt ttgatataga tggtactctt
     9601 tatagagact ctcttatggt tgaacatttt aaaaaattaa ttaaatacga tataattgac
     9661 caaaaaaatt ggtataaaca tgctagagac acttttatgg attgggataa aagacaaggt
     9721 aattatgatg attatctatt agaaatatgt gacctttatg ttgattcact aaaaggatta
     9781 gataaaacct gtatcaactt tacaagtgac caagtaatta aattaaaagc agatagagtt
     9841 tataaataca cacgttcaag gattgagtgg catctaaatc aaggacacat agttatattt
     9901 atatctggta gccctggatt tcttgttgaa aaaatggcca aaaaatataa tgtaaccgat
     9961 tttctaggaa gtgattatgt ctttgaaaac aatatattta caggcacagt tattccaatg
    10021 tgggattcta taagtaagaa taatgctata aacgattttg ttgtaaagta tgatttagat
    10081 ttatcaaact cgtatgccta tggagatact aatggtgata taaatatgct aaaaagagtt
    10141 ggaaatccaa tagcaataaa tccaacaaag gaacttttat ctcaaatatc acttgatgag
    10201 gaaatttcta aaaatgttga gataatagta gaaagaaaag atattattta ttctctatct
    10261 acagatgtaa atatagtgga tatttaattt atactatcta ttttaaaaac ttatattata
    10321 tctaatattt atatctaaca tataaaagct ttgactaaaa atcaaagctt ttatatgttg
    10381 cactaagaat atattaaatt tagctttatg ctttaatgta tattcatata tatcatttac
    10441 acttatctaa actacaattt cattaaaatt aaatataaaa atcaactatt attttttaaa
    10501 tagcatcttt cttaacctat tattgtaata acctataaaa gaactgtaaa catagtcata
    10561 tactataaag gctatctcaa atgaacctat aataaatatg tttattggaa tataaactat
    10621 tgttcttaat aaaaaataca atataagtat cattatattt gcaaatacta atttcataaa
    10681 atactcaata tatatagacc tatctttttc tataagatat tttacaatac catatattcc
    10741 aaaggtaagt gaatatacaa tccattgaga cttacttgcc atcactataa atcctaaaac
    10801 tatagaacct atataaaaag cgattccact cttaaagccc cattccatta ttacaattga
    10861 tactaaaagt gatgctaacc ccataataaa caatgtattt acaggaataa tatttgtaag
    10921 agtcaacaat atcacattta atgctaataa aactcctcca taagcaactt ttatactcat
    10981 atttatcatc ctaacaacaa gttattaaat ctccaccaaa acattcacaa catgaatcta
    11041 aacaccaaag cttagcacaa ttttcacaac aatcgtcacc tagacaacaa cagcaatcat
    11101 cacagcaaca gcaacaacat ccatctaaat cattacgtct tcttctatta taattgtcag
    11161 ccctattgct ataatcattt ctatattgtg tataacttct aagtgcatca ctatattcac
    11221 tattttcagg ctccataaat tttgctcttt ttaagaagtc ttcaccttct tcataatatc
    11281 ctatgttcat tgcagaaaag ccagttaaat aataccactc tgcacatttg tcagtcaaag
    11341 tttttaaaaa atcatatgct tttttaaatt cactattttc tatgaatgat cttgctctca
    11401 taaaattttc attattataa gtattactgt acattctttc cacctctact ttctaatata
    11461 ttaatatatc ttaaataaac accagaataa attatatttt ctattattcc aacattttta
    11521 tttagattta gattatcaat tcccctagct aaaaaaccta atgatgttac tattattttt
    11581 tttacttttt cttttaactc atcattttta tcaatatact ctataaatgg attatatctt
    11641 cctttcttaa aatcttcatc taaatcttca taggcatcta gtagatatat atacttacct
    11701 atattaaaac ctatcattct taagtttctc tcattttcat cttttttata gacaaatact
    11761 tcacccatta tatttccaaa tatactagat acttcatcta tatttatatt tttatctttt
    11821 tctaacttat ttaattcatt tagttgttgt tttatatact ctgctttttc aggatacttt
    11881 tcatatgcta attttaattt gtttttatat atataatatg ccaatttatc ttttatgcgt
    11941 ttatcatcca ttaaattatc ttccaattta taatacgtta aaagaatatt catacttgct
    12001 gcatattctg taatatcatt tgtaattacc tttttctttt taaatggatt tactatacac
    12061 acttcctctg tacttataga ctctgggtta tatactgctg ttaataaaac aattaaaaat
    12121 gttatatcat aattcaaaga aaacctagag atttctccat gatttctttt taagtattta
    12181 catagtccac aatagtaagc tttgtaataa tcatattctc taaatgttaa atccattttg
    12241 tttattttaa catatccaaa cataaatact cctttttgta ctactttatt atataaatta
    12301 tatttatttt ctaactcatt tattttatag tgcattatgt aagtttttac tctttttact
    12361 tactgatgct atatagtcct tttgacttac tacatattat ctctaagtta cttatatcat
    12421 ttaatagtat attctttgcc tttgaacaag tcatttttta tcccttatta tttaaggttc
    12481 aatcaagatt taacaagcta tatatttaca ttttttgtaa gtctaacata attaatatat
    12541 taaaaagatg tatttatcct atatgtttat gtgttaatta gactttagta accttaattg
    12601 aatctattgt ttcattggtt agaaaatgag tgtatgccat caaacattaa ctcgctcctc
    12661 ttttattttt tgttaattat tattatatca ttaataaaat tataattcat ttattttata
    12721 tgtaatcatt aataaattct tatctacttc tcttttcttt tttaagtctt ctatattcgt
    12781 ctaagaatgt tctgttaggt atagttactt aattacaatc tcatctattg ttatacttac
    12841 atctttattt tgctataagc tatcatttat tacctatttc ttcttatttt ttctatttta
    12901 gtggacattt aatttatgac aaataaatat atttatttga ttatatttaa tacaaaacta
    12961 ttcttataac ctttttaaat catatttaac atataatatt aagtaacata cttacttaag
    13021 aggaattatt aaatgataaa atttgaaaag ttaaatatta ttaacaataa agatatgaaa
    13081 gaaatactag atacatggga atcatcagta agagcaacac atgacttttt aagtgaagaa
    13141 aaaataatat caataaagcc tcaagttaaa gaaggtgcta aatatgttag taatttttta
    13201 tgtgtaagag acaaaaaagg tcttataaaa gcttttatgg gaatacatga ttttaaaatt
    13261 gaaatgttat ttgtaggtaa ttatagtata ggaaatggaa ttggaaaaag acttgcagaa
    13321 tatgcaatag aagtattaaa tgtaaattat gtagatgtga atgagcaaaa tcaacaagca
    13381 ttaggctttt ataaacatat gggatttaaa gtctttaaaa aatctgagtt cgatgaacaa
    13441 ggtaatccat tccctatact gcatatgaaa ttaaaataac aatataaact atagtataac
    13501 taaataaata taaatagagt aataattgaa aaatattact ctattatcat aaccttactc
    13561 tcacttatct tgttataata attatataat atatttttag gtcgtttaaa ctgtattttt
    13621 agtttttaaa taacaaatat tttctattgt tgatttccaa tgattttata caaaaatagt
    13681 ataattgtta tatgttacta tagaagtttt atttattttt cttactgtct aatactactc
    13741 caataactaa tccaaaggat aaaccaatag ctaagttttt aaataatgaa cccaaaataa
    13801 caccaaaaca taatcctaac gccaaataac ttatattttc atttttcata tattactcct
    13861 ttaaatttaa ttgtaacatt ttaacttttc taattgattc ttttgaagtt ttgactatat
    13921 caactaaacc tcttatctca tagttattac actcacttag cactcctttt actaatattt
    13981 taagatatct cccctaattt ataaacagta gtagtaaaaa atggttttta aacatatatt
    14041 ttcaaagtct aatatatata aaactatttt tatgaatctt tactaattcc aactgctctt
    14101 aaaggaccga gtaacgacat tctaacaagt atcagtacca tttcttcagg gtaatctata
    14161 acctaactaa acctaaaaaa taaaattaat tataaacaaa aaataaatat aataaaaggg
    14221 cttacaaatt ataagtcctt ttatcgaaca taaaatcaaa ttattttcct taaataggaa
    14281 actttaaaaa tatattctaa ttattatatc ttagattgaa aataattaga aagttaagtt
    14341 aaaaataatg ttaatttata tatttatcct aattcataca attcaatctt acttgtatta
    14401 aattactctt caaataattg gagtatattc aaaactacct acagtatact tcaattcttt
    14461 ttttgcttta tttataacca attcatcaag cattacatca tttattgaac atgatataga
    14521 tttagatgcc aataacattg ctttaaatcc catatcagaa cctgcacttg cacaagattg
    14581 ccatgtatgg gcagaaatac caataggcca aactgaacaa gaacactgtg ctgtaggaat
    14641 tatgtaacta acatctccaa tatccaatga taatgatata cagttattat gagtaaatgt
    14701 atcatccgta acttcatcat gtaaattcat cttagtaata tccttatttc cacttaagac
    14761 actagaaaca ctacttcttt tatctataga cactgtctca gctaatttat ctgcaaaata
    14821 gtagtcatct tcattatagt tttgtacacc tactaatttc atattttgtg atagaatatc
    14881 tgttaatatt gtatttggaa tatagtcata tactccactt ataatatcat attccatact
    14941 agtaccagtc attaatgctg ctccttttgc tatatcaaca atcctaccaa atacatctct
    15001 tacatgctcc attttaactc ctcttatgta ataccacact tctgcaaaat ctggaactat
    15061 atttggcatt tttccaccat tagtagttac atagtgtact ctacaactat caaatatatg
    15121 ttctctcaaa taattacaac ctatattcat aagttctact gcatcaagtg cacttcttcc
    15181 gttataagga gcttgtgctg catgagcact aatcccatta tatctaaatt tcacagagta
    15241 attagcaagt gttgggattc ttataggagt atttatatca aatggatgcc aactaaaagc
    15301 acaatcaatt ccatcaaaaa aacctttttt tatcatcaat acttttccag ataaatcttc
    15361 ctctgctgga catccatagt attttatagt ccctttcagc ttttttagtt tgattaattc
    15421 ctttactgca attacagctc ctattgaacc tgtacccagt aagttatgtc cacatccatg
    15481 acctgcacca ccatctacaa taggttcttt aatagtggat actttttgag acaaaccatc
    15541 taaagcatca tattctccca aaactgctat aactggtcta ccttctccat agctagccgc
    15601 aaatgctgta tccatacctt ctaattcatc tatactaaaa ccatattttt ttaagtattc
    15661 tttttgtaaa tttgaagcat aatattcttt atactgaagt tctggatttt tccatatctt
    15721 atctgatata tctcttaatt ctccctgtat ctcatctata tattccatca aaaacttttt
    15781 atttaaattc tccatattaa ttatataaac ctccctgttt ctaataactt atcgattatc
    15841 agccaaaatg actaatttta actgatttaa aaatattttc taccatacaa ccattttata
    15901 caataaaacc tcttgacttg taagtttcat tttatcaagt aagaggtttt caagttttat
    15961 aaaattttta gcatgttaac caatcaagca tattaaccca taatttatta tagtatttcc
    16021 aatcagtaaa ttcttttgga ccccagtgag gtgcacagtc agaactaaag atagcagatt
    16081 ttccttttcc aaactcgcca acagctacaa atgggtctcc tccaatagtt gctaaaacag
    16141 gacaattatc tctagctact gttttgttat atcctaaaaa ctttggccat tctgttggta
    16201 taccttttaa tactggatgt tcagagtcaa taacttctgg aattattcct gctggttttt
    16261 caactctatc atctttatct atcattgtta ttggtaatac atctttgata gcagtttctc
    16321 caaatctagc ttttgcatct attcctgtaa atgacatata tccacctacc atgattaacg
    16381 ccccaccatt atttacatac tctttgatta gttcaagtct atctggatta ctattgcaat
    16441 ctataaaagt actattagat aataagaacg tattagaacc tatatcagat aatattatac
    16501 agtcatactc atttaactct tctaatttat acggaaaact atctgctgct acatgtgcag
    16561 gcatatgaac tgtctcatat cctccagatt ctattgcttc tttcaaccac tttacagctt
    16621 cttcataatg agttgtaaca aacgtatcaa acccttttat atgagttata ttttttaccc
    16681 aagattcccc tactattaat acttttttca tttatatatt cctcctactt ttataagttt
    16741 aatattagac ttgcatacat aagtatacta tctaaatact ccttaatttc aacatattca
    16801 tcaattgaat gacattgttc tattgaacct ggccctaata taagtgttgg tatttcagct
    16861 aaatttttca ttaatctcgc atcattgcca gctgttgaac cttttatctc taattttttt
    16921 cctagagttt cttctagtat tttatgtgca ttcactacaa aatcagaatc cttgtccatc
    16981 tcaaaaccta accctgattg atatatgttt atatttggtt tattatcttt aagccataaa
    17041 tctccattag cccttgtcat tatgacattt gttacatcat ttacaacttg ttcataactc
    17101 attatatttg gcaagaagtg tacacataaa ttaaatacac acttatctgg cacagtagaa
    17161 cctgctgttc caccatttat tacacctaaa tttattgtag gtgaaggtaa tagtggatgt
    17221 ttatatatca taagccagtt atgttccaac tcttttatat cttgaagcaa caacatagcc
    17281 ttctcaattg catttacacc ctcccactta ctaccacagt gaagagacac acctttaacc
    17341 tcaacttcaa agaatacaaa tcccatatgt gcaactatta agttttgttc tgatggttca
    17401 caaattatgc aacaatctcc accaatacca ttcataactg catttatggt accatttccg
    17461 ccaccttctt catctactac tgacattatt tttacattac caggtacatt tagaccacta
    17521 tcttttataa gctttacagc taatattgat gctataagtc cagatttcat atcagcagtt
    17581 ccaagaccat aaagttttcc attatcttct gtagcacgat atggattata tttccactta
    17641 cttatatcac ctggtggcat agtgtctaca tgaccattaa atacaattgt cttaccaggt
    17701 aaatcatcac tatacttaca aattaaatta tatctatctt gataattgtg acctagattt
    17761 ccttctttat atatattttt tgctttttta attaattctt cgctcatttc ttgtctatct
    17821 acactaaaac ctatgtaatt tgcaagtttt tcaatatatt cctgtccttc tttttcaaat
    17881 ccacctagta ttccatgacc aacatcttca gtctttatac ttacaagttc ttttaaataa
    17941 tctatgtatt cttgtttttt actatttaat gtatctaaga gaattttttt catatttcct
    18001 cctaagattt atttttacat ttaaaatttc aagtctaatc tcatctattt aagccatagt
    18061 aaactttttc acttagcata ttcccattca atgtataaag atgtatgcaa attaaagtat
    18121 aaaaatatat acaaaaactt aataattttt atagtttata aactaatatt taattactat
    18181 ttagatttag ctgatttttt taattttata ttatttatcc atatagcaaa taacattacc
    18241 aaacctttta taatcatttg attatatgaa gatacactta ataagtttaa tccatttcct
    18301 aatattccta ttattccgac acctatacat gtacctacta tagaaccaga accacccatc
    18361 atattagtac caccaagcac aacggctgct atagcatcca attctgctcc atctccaaca
    18421 gttggtgaac ctgatgccaa acgtgatgat agtattatcc cagctaatgc tgttagtagt
    18481 ccagctagtg cgtatacttt tactataact ttatttactt ttactccaca taatctagcg
    18541 gcctcttcat ttccacctac tgcatgtaca tgacgtccaa aattagttct ttttaataga
    18601 aaatgtcctg ctattacaac tataatcata attataactg gtattggtat tggtccaata
    18661 tatcctctac cgagcatctt aaatgcatct gtatttactg atataggtct tcctcctgta
    18721 aatacaaagc ttgccccact tattatcgtt aacatggcta aagttacaat gaatggaggt
    18781 agtttttgtg ttgaaataat tgcacctttt atgactccta aaattgtccc tattgctaat
    18841 gcaagtatca tacctaccca tataggtgca ttataattca ctactgttgt agcccaaaat
    18901 gtacctacta aagcaattac tgctcctaca cttaaatcta ttcctcctgt aattattaca
    18961 aatgtcatac ctactgcaag tattgcattt attgatattt gtcttccaac attaattaaa
    19021 ttatctaaag ttaaaaagtt agatgaagct acactcatta ttataaaaat tattacaagt
    19081 cctacagctg cactcccttg gtctctaaat acacttgtta attttgtcaa taatggagaa
    19141 gatttttctt ctttatgctt agttattgaa ctcattaatt ttacctccga aagccaattt
    19201 taatacatta ttttcaacca tttcttcacc ttctaaaaat ccacttatat gaccttctct
    19261 cataaccatt attctgtcac tcattgatat taattctggt atttcagaag atatcattat
    19321 tatacttaca ccactctcca ctaaatctcc cattagattg tatatctctg ccttagcatt
    19381 tacatctata cctctggttg gttcatccat aatcaatata tctggttgta ataaaagcca
    19441 tcttgctatt atagcttttt gttggtttcc accacttaaa ctttgtataa cttgattcat
    19501 atgtggtgtt tttatcttta aagcttctat gtatttttct aaaatctctt tttccttttt
    19561 tctatctata attgatttaa acttagatat cttttttaac ccagccatag ttgtattttc
    19621 tttgacactc atttgtaaaa acaatccagt accttttcta tcttctgtta ttaatcctag
    19681 tccattttct attgcatctg atggagtttt aaactttact tcttcattgt ttaaatatat
    19741 ttttccagaa tcatatgttc taagtccaaa tatagcttct actatttcac ttctcttact
    19801 tccaactagt ccacctattc ccaatatttc accttttctt atattaaagc taatatcatt
    19861 aaaaacattg ttcttagtta gattttccac tcgcataatt tcttctttta tttctacttt
    19921 tctttttggg aaaaattggt ctagctttct atctatcatt aaattaacta attcttcctc
    19981 agacgataca tctttggtat tcaaagtcga aatcgttttc ccatctcgca ttatagttat
    20041 tctgtcacaa atctcaaaga gttcttccat cctatgggat atatatatta tactaactcc
    20101 actctctttt aaatcattta taagtctaaa aagtatttca gtctcttttt tacttattga
    20161 agaagtaggt tcatccatta ttaatacttt agtttctgat gataaaactc ttgctatttc
    20221 taccatttgt ttttgagcta tactaagctc tttgactaaa ctatttggat ttatatctat
    20281 tcccatatta tcgaaaatct ttttagcctc ttcattcatt tttttgtcat tggttaagac
    20341 tttgccattt atttcttttc ccataaaaat attttgtgct actgttaatt cgtcaaaaac
    20401 tgttagttct tgatatatgg catttatacc tatcttttta gagatatctg gggatatatt
    20461 ctctatttct ttgccttcaa aaataaattt tccagaagtt ttcgaatgtg ccccagttaa
    20521 aatctttatc attgttgatt taccagcacc attttcaccc agtagtgcat gaacttcgcc
    20581 atgaaataac tcaaaattaa cattatctaa tgctctgaca ccaggaaatt ctttagcaat
    20641 attacttaac ttcagaatta tattactcat aatgacactc ctttttaaat aggatttctt
    20701 tcaaataatc tcattttatt ttactaattc ttaaagtctt ttatattttc cttatcataa
    20761 agtaaagctg gtactggtat attcttttct accttttccc cttttatagc ttttactact
    20821 gcttctacag ctaaacgtcc catctcttta ggttgttgtt gaatcatagc aagaatagtt
    20881 ccatctgtta ttccacttga tgcttcttca gttaagtcaa atcctacaat cttaacatcc
    20941 ttattttttg cagattcaag tgcagcttta gctcctagta cactattttc agctgttgca
    21001 tatattatat ctacatctgg attagattga attaagtttt ctactgtatt aagagattct
    21061 tctctgtcat atcctggttg tgaatttaat atcttaacat ttgctgaacc ttttaatgca
    21121 tctttaaatc catctattct ctgcatttgt atttgagatt ttaaatctgt cactattgct
    21181 atttctgact ttccacctaa cttttcatct atgtattttt tagtgtattc ccctaatttt
    21241 ttaccagcat cataactaac tgttcctaca taacttgtaa tattttcagt atttaataca
    21301 gcatctactg taactactgg cattttttca aatgctcctt ctactgctgg tactatagcc
    21361 gaattatcag ttccaagtac tatcattcca tctacattct gttgtataaa atcttctatt
    21421 gctgatgttt gcttagctac atcttggtct gggtcattta ctataacttt cacacctaat
    21481 tctttagctt tttcttttat tccctcatca atatctataa aaaattgatg ttgaagagtt
    21541 attgtagata ctccaatagt taaatctttc ttgtctgaac cacctttatt atctgattta
    21601 cttggtcctt ccattgaaca ccctgttaac attcctattg ctagtattcc agcaattcct
    21661 aaagataaaa tttttctcac tctcataatt aacctccatt tttaatagtt ttaaatttga
    21721 acaaattaga tttttattta ttatttaaat acttttcaac atcttcttta tagggcatac
    21781 cagattgtgc acctagttta gttacagaaa gacctgatac tactactgca tattctactg
    21841 ctgtatataa atttttccct tctgttatag ccactgccaa tgctccatta aatgtatctc
    21901 ctgctcctgt ggtatcttga acatcaacac ttatacaaga tacatgcttg atttcctcac
    21961 catcataaaa tctagctcca ttttttcctt ctgttattac aagtttattt ggatattttt
    22021 ttaatacttc ttctatccct tcatttgtgt caaaaactat cttatattca tgttcatttg
    22081 gtgttaagta agttactttt tctattatat cttcatttag ttttactgct ggtgctggat
    22141 ttaataaaac cctaatccta ttttcaaaac agaaattcac tacataattt attgttttta
    22201 atggaatctc taattgtagc aatactatat ctgcattctt tatctcttct tcattctttt
    22261 taattaattc tatatctact agctcattag tccctggtac aacaacaata ctattatcat
    22321 tttcacataa tgttataaca gcaacaccag aacttttgtg attatttctg gatactaagc
    22381 tataatcaac tttattcgtt ctaaaatttc ttattaactc ctctcctaag ccatcttccc
    22441 ctatgcatga tatcattttt acatttgctc ctagtttaga agctgagata gcttgattag
    22501 ccccttttcc tccaggtgat gtaaaaaaac tattacctaa tactgtttct cctttttctg
    22561 gtcttttatc tacagaacat accatgtcca tgtttacact tcctatgaca actatgtttc
    22621 ccattacatt actcctttac atatctattt atgtctctcg tgctgttatt cactttcttt
    22681 tgttgaatct cttatgacca attctggttt gaaaatcaat tcatcaaacg attctttttt
    22741 atctcctctt aaacaattta tcaatatttc actggcttta tatcctatat cataagacgg
    22801 ctgttttact gtggtaagag aagggcttat tagacttgat gtttgaatat catcaaatcc
    22861 cacgacactt atatcatttg gtatacttaa tcctctttct ttaagacctc ttatgacacc
    22921 tattgcaatc aaatcatttg cacaaaatat tgcatcaaaa tctatctttt ctaaattttt
    22981 tataaattct tgtccccatt caatcttata ttttccttca aatacataag caggattgta
    23041 gcttaaatta cactcgatta gagccttttt atatccttct aatctatcta ctgctatttc
    23101 attttgaagt ggaccactta ggtatattat tttcttattt ccaatatcta ataaatgttt
    23161 tgtagctata taggcccctt caaagttatt tattttaaca attcctttta aggtttcttt
    23221 gttcatactt atcttcttat caatcaagac agttggtatt tttatatctt tataatttgc
    23281 ttctttccag tttgtatttg aagatgctgc aaatataatt ccatctatca tcctctctga
    23341 tagtgttctt attccttcat attctttttt agcatcttca tctgtattac ataaaattat
    23401 attgtaacct tcttcattag ctttgtcttc tacacctctt gatacatctg taaagaatgg
    23461 atttcgtata tctggtataa gaagccctat agtattagtt tttttagtta ctaaactttg
    23521 tgctaattta tttggtacat aatttttttc cttcattatt ttcaaaattt tttctcttgt
    23581 tgcctgactt atattttcat ctttattatt aatgactttt gacacagttg ttttagaaac
    23641 accagccaat tcgccaattt cttttatagt tatactcaag aagttcaccc ccaaaattta
    23701 ggaaaccgtt ttacttaacc ggttacctag atactatcac atcttttttc catttgcaac
    23761 cattttatat aaattttgat atttttccct tttttcaaaa tattgcaact tataattcaa
    23821 ttttaatatt taaattaatc tgcaaaaaaa aattaatata cttttataat tatctaaaag
    23881 tatattaatt aatttaagca gtatttatta aagttcaaat ccttgctcta taatcatttt
    23941 tttatcattt tcaatcccac ttccaacagt tgtcaaataa tcaccaacaa ttgcagaatt
    24001 tactccccca ttaaatccaa ctttgtcata tccctttaat gatagccttc caccagcata
    24061 ccttatgtac acattaggaa ttataaacct aaatactgct gttgttttta gtatttccat
    24121 tggttctaat acatcataat tttccattgg tgtattctta ataggattta aaatgtttat
    24181 tggaaatgat tttacattta attctcgtat ttcaaaagcc atttttagtc tctgttcttc
    24241 actctcattc attccaatta taccacctac acatacatct agcccagctt ttttagcatc
    24301 ttttatagtt tttatcctat ctttatatgt gtgtgttgta catatattat gaaaattatc
    24361 tctacacgtc tcaacattgt ggtgatatgt agtcacacct gctgacttca acattttagc
    24421 ttgctcataa tctataatac ctaaagatgc acataatttt aaattagtat tttccctcaa
    24481 tccttcatag atatttaaaa tattattaaa ctcctttcct gacatacctt taccacttgt
    24541 aaccaatgag aatctatgca ctcctttact ctccatctct ttagctctat ttaagatttc
    24601 attgtaatct aataatgaat attcttctac accagtttta taatgagatg attgagcaca
    24661 aaatttacag tcttcactac atttgccaga tttagcattc attatcgtac ataaatctgc
    24721 ttttcttccc ataaaatatt ctcttatttc atttgcagat tttaatagaa tatcaaaatc
    24781 gtttttatta tttgtatcaa ggcttattaa attaagagct tcttcataac ttatttcttt
    24841 ttctctcaat actttatttt ttaattttat tatgtatttt tccatatatc ctcctatttg
    24901 tcaacttaat atttttatgt agtttactta ttatatatag tattgcatat ataacattta
    24961 ttggtcaact ttattttaga cacaagttta ctatacacaa aaaaatactg cttcaaaata
    25021 atattagtaa atggattata catattatta ttaaaacagt attttttatt agaatataat
    25081 tcttactggt ttataaaact atccttgtct taactccatc caagctttcc attgtaataa
    25141 cctctaagcc ttcatattct ctaaatagcc attttaaatc cttcatggct tgattaacct
    25201 gactttttgt tagattattt ataaaatatt cttttctagt ttcctcatac tctttgctag
    25261 tcaaaaagtt tcttagaaaa ttaaacgcaa atacacttgg aatattgttt acattagcct
    25321 tcttgctgtt ttccttctca tagtaaattt tcataaatgc atactccttt gatactttta
    25381 gatttctctt acaataatta tatgcattta agtgctacta ttcttataat tttattaatt
    25441 catattcttt acttcccata cccatttcac atgcatagtc tattattcta gttccattta
    25501 catgaggatg ttcatgtttg aaaacatcat gacccatttc cttacaaatc aaatcatagc
    25561 atgcttgttc aatagctaca gggtctgttg atgctaatat tcctatatca tgggcaattg
    25621 gtctacctga ccaaggtaca caatcacaaa gtggagttac attcataaca aaagttatat
    25681 accctacctt gtctttttta tttgatactg ctccataagc gtattctgcc atcttttcga
    25741 caaacacatc tgagtcactt tcccattgta tagttactgc tctagttgga cagacagttg
    25801 gacattctcc acatccataa catacatcag aatctataac tgctttttta tccactatac
    25861 ttatagcttt tgtaggacaa gaattaacac actttccaca acctacacac ttcttttcct
    25921 ttactactgg agtaacatca gaatgttgca tttgttttcc tgctgcactt gcacatccca
    25981 tagctaagtt ttttaatgct cctccaaaac ctgctgcttc atgaccttta aaatgactca
    26041 ttactatcat tgcagaagaa ttataaattt ctccacctat ttttacactt tcaaaatgtt
    26101 ttttgtctat ttttacattc tcataatttc tactatataa tccatctgca attattactg
    26161 gtgcatttac aactgcataa gcaaatccat tttctatagc agttgtaaga tggtctacag
    26221 aatttgttct actccctgta tacagtgtat ttgtatctgt taaaaatggc tttccttcgc
    26281 agtctttaac cttatcaacc acttgtctaa ctgccacagg actcatatat gtagtatttc
    26341 ccttttctcc aaagtgaagt ttaattgcaa cttggtcatt cttttctatt aaatctttga
    26401 atcctgcttc atcaaataat cttcttacat tgtttggtac gtttttattt tgcgaatttg
    26461 agtataaatc acaaaaatat acttttgact tttccatatc tacacccctt taaaatattt
    26521 acttatacta attttatcac ttttaaagta atttttagta tattttaaca taattaaata
    26581 ttcttatatt tatttgatac aaataatatt atttgtatta ttttctacac ttaattctat
    26641 atattaaaaa tcaaaaagac ttgaaactta agtccaagtc tttttgattt ttaggtgtag
    26701 tttttaacaa cataagatta ctttaccttg tgataaaggt attattaaac tggtttttta
    26761 ttaaataaaa taacagctgc tagtaaacat actacagaaa taagaactgt tataataatt
    26821 ggaataataa attctggcat tgccactttc cctgaaatca agtctatatt ttttgacatc
    26881 aagaaattag gagtataagg tgcaatctgt ttaggaatag aaattaaact caataaaaga
    26941 gttgccacca atacaagtaa aacactggca aatgctggtc taaatagtac acacccaaga
    27001 attaatacac taatatacat aattcctata agccataaat taaatgctga aaatataata
    27061 tgattaagat ttgcagttgg ccaaaaatat attgtatagc cataagcaaa taaaaaactc
    27121 aaccaaaaac caattgtcat aataatcacc gtaacagaga atttagacaa tactatagat
    27181 gaacgtgata aacctttagt cagcataata acaagtgtcc ctttagaata ttcatttgat
    27241 aaaatattac aaaaaataat tagagttaaa ctcataccaa gaccagagat attgctaaaa
    27301 aattgtgtcc aagaatctaa agcagttggt gcagcagtaa cttttaaagt cggtgcaaaa
    27361 tgagcaatca agtctggcat aaattttgca gaaataggac tacttatacc aaatattaga
    27421 aataaaataa ttaaactaaa taatttataa tttcttaaat tctctttaaa ttcttttttt
    27481 gtaaatgcta tgtattctct cactttacta cctccaaaaa taaattttca agtgataatt
    27541 ctattagttc catctttact ggacaaagat tcatttctgc gagtgtagat ataactgttt
    27601 tttgtattgc ttttatatct ttaccatgca aaacaatttc catattagtt tcttctgaat
    27661 cttttagtaa ggatttaata ccatcactag acttaaactt ttgaatttca tcattacttg
    27721 caaactccag taaaagtcta tcctttccgt gcatactttt tatttctgaa agtgtaccac
    27781 taagggcaat gcttcctttg tttaatatag caacatggtc acagattctt tcaacatctg
    27841 atagtatatg tgttgaaaat attacagttg ttgagtcttt gattttaagc ataatatcta
    27901 atatttcttt tcgaccaaca gggtcaagtg cacttgttgg ttcatcacaa ataagtaatt
    27961 tggggcgtga aagcaaagct tgagctatac caagtcgttg cttcattcca cgagaaaacc
    28021 cacctattcg tctcttttca tttccaagac ccacaagtga taacaactct tcactcctta
    28081 tctgtatttc tttttttgaa agtcccgtaa tttcaccaca aagagaaaga tattcaagtg
    28141 gtctcatata gttgtaaaac tctggaacat caggcagata acctacatga cgattagaac
    28201 ttgttttccc ataggtcact ttctcaccac aaacatctat agttccacta tctggtttaa
    28261 gtaacccaag taccattttc attgttgtag tcttacctgc accattctta cctacaaacc
    28321 caaagactga cccttctggt acagtaaaat ttaaattatc tattatcttt cgattaccaa
    28381 aaccttttga taatccattt attgagacaa tattcatcat tcttcacctc ttcctattgt
    28441 aaaatacaaa attggtccta ttagactaat aaacagtact acaataatcc atattgcctt
    28501 atttccaatt ttataatttg gatgtctcaa tacatgaaca agtgcagtaa ttataagtat
    28561 taaatctaaa ataataacag gaaccaaaag tggtaagtat tccatcaaat tttccataat
    28621 cttattcctc caataaagat aatttttcta taagaaattt gtattctttg ttttcactta
    28681 atacagaaaa cattggatta tttttaatag cagcaacaat acctgctttt acgattgtat
    28741 catcacgtgt aatacctgtt ccaatttcag aaatccatct gtcaagttta ttaaaaaagt
    28801 catccccatg tattataatt gggtatatat cacgtgttgc taaatctaca tattttcgta
    28861 atgatttaat agctccttca ttatcaccat gtattaaatt aatttgtgcc tgtgttaaat
    28921 atgaagaaag cataatagca ggagataatg tatcaagctc aaatatatct gaaattgcta
    28981 gaatacgtct ttctatttcc ttcatttgta atttatctgc attttgcaaa atacccacta
    29041 ggttttggac taatgatata aggttctgat atgcaccaag ttgaaatgtt tctctcgctt
    29101 catccatttg accattcatc atttgcccct gtgcaagaag tatagattca tttatcatgg
    29161 gaaaattgct gttttgcaat aaatcaatca cttgtatggg ttgatttaac aatatataac
    29221 aagttgcctc catacttttt gcttgacggc aaatatctat gtcattacaa gtttcctgta
    29281 tacgaataaa tatttctaat gcttcactaa ttaatagttc acgctttttt tcatctacaa
    29341 tatcatagtg attaactatt aaaagaccca tccgaaaaat caaaggaaaa catgaataat
    29401 atttttttat gatttctctt atttcatcca tgacttcatc aaatggttta accgtaaagt
    29461 ctttgcacag tttattatac agtttattaa tatcttcctt catcatttga ggttcataac
    29521 atattaactc atcaacggta atgttgaaat atgtcgcgag ttgcggtaaa aaggttatgt
    29581 caggatagct ttgtccagtt tcccatttag atacagaagc tttagaaacg cctatataat
    29641 ttgcaagttc atcttgtgta attccttttt ctttacgctt taaaatcagc gtttttgcga
    29701 tgtttaattc attcatgtca atccctcctt atgtataatt atatccttaa acatgtaaag
    29761 ctaacaagcg atttgaagtt gacttttgac gaaaaaatca accacagaga aacaaataaa
    29821 caagtatata aaatatatat ttaaatttat ctttaatctc ctttgtaaaa tatgtaaact
    29881 ttgtaaataa aatccatatt atataaaact aatagttaat ctatctaaat acagttttta
    29941 tttatcacgt ttttaaatat tttgtgtaaa acctctttta atataaacac cttattttca
    30001 tttctcattt catattcttt ttaaaatact gaatataact cttcgatttt ccatttaatc
    30061 cttttataaa gttattattc gtatttattc ctctaattat aacaggcaac tatcatgtat
    30121 tggtatttgt ctattatata aaaatctata aaagaaaagc agtcaatcat ttagattaac
    30181 tgcttgtaca tatatcttat taaactgtat aaactataat ttttattatt ctccattttc
    30241 atcccaagtc ataaatcccc atccactatc tggataaata tcgatagact tttctctaat
    30301 catataacca actttataac actcttttgt tattttatta gatagtatac atccttctcc
    30361 atttatctaa ttccatttaa ttaacttttc ttttcaacct taacttttat aaaatcatct
    30421 tttttaataa tttcatactt attttctctt caacatcatc aaatgagggt gtctcaaaat
    30481 taacttttta cttcataagg cactcttttt tgtatgaagt aaaatatatt ttcatcattt
    30541 caacaataag taagaggctt atctctattt tgagacagcc ccatttgatg gtgttgaaca
    30601 tttaaataag gttgaactcg ttttatctct ctttttaaga tatctgtaaa tttgcacata
    30661 aagtctcttc ttcttttaca attctaatag ttttattaaa gccaaagtgt atatctccat
    30721 attctttcgt atatgattta ttggtactct ttcatctact ccatgaccac cagttttata
    30781 cgctaagaac ttaggtccaa acgccaccat atctttaaac caacttgcat atgtgccacc
    30841 acccatagca attgtatatc cattttcatc tgttacttct tcataggctt ctaataattt
    30901 tttaagtact aaattactct catccacata atgtaagtct ttatgagcaa taacctctat
    30961 tttccaacca ttacaaaaaa cctttttgaa tctatctatt atattgttta aaactatatt
    31021 tttaccatac cttatatcta actcacatac gattttttcc tcatttactt ttaatattcc
    31081 taaatttaag gtagtgtttt tgaatacctc atcatattga ttaatcccca atgcatctcc
    31141 ataataatca ctacaaaggt attttgaaac taattctata tcttctctat gctgaaaatc
    31201 catattatcc tcaactatct ttattaccat tttagatata gcattttctc ccttttcagg
    31261 attacttgca tgtacagcta ctcctttagt agttaactct gaaataattc cattaataat
    31321 ttttgcagta caatgagatg gaacaacatt ttctttagtt cctccactta ctattatgta
    31381 aggtttactt ttgtcaattt taatttcttt ttctatcctt atatgaaccc ttcctttttc
    31441 tccattaacc actggataca tagcatctgg agtaaatccc atacttggaa tttcttcacc
    31501 tttatctaag taatatttga tacatttcat acctgtctcc tcatttgtac caaaaattaa
    31561 tcttattctt ttattaattg taggatacat atcctctatg tttttcaata catgtataag
    31621 catagataca gcacccttat tatctatgac acctcttcca taaataatat tgtcaattac
    31681 tgctcccgaa aatgggtcta cactccaccc ctctcctggt ggaactatat ctaaatgcat
    31741 tggaatccct attaattcct ctccctctcc aatttctgca tatccaatat aattatcaat
    31801 atttttaaca ctaaaaccta aactagcaca gtaatctaaa gttttatcta aagatttttt
    31861 aggtcctata ccaaaaggac attgtgaaat aggttcacct tgttgtgaat caatttttat
    31921 caattctttt agaaattcta tggttctatt taaactatta tctacacttc tgcttatatc
    31981 cataaatcaa cctactttct atctttttaa agcctgtcct tgaagaacag gcttttagca
    32041 ttaaaaatta tttttttgta aatactaatt cacaaacttc atcatcatga gcacattttt
    32101 gagcaaaatc taatttaaca ccttgtgcac agtcaactct tccataatcc ccataacaag
    32161 ctaatctaca aatttctttt ctttcttctt tagttaatcc atattctttc caagcagcat
    32221 ctaatggaca gtgatggaat tttaaaacgc cattctcatc tgtattttct actaattcca
    32281 tttcaaacac tttttgtcct ttactgttat ataacatttc tgccattttt cctggagtat
    32341 ctgctacgtt atatttttga cctctcattt taccaaatcc atatattgct tctttacata
    32401 tatcatctgg attagctcct aatttttcag cttctttcat aagaagatag aaccatactg
    32461 ctctttctcc tgttgctcct ctaattcctt ctaaatattc cttttctgta tacatttttt
    32521 ctgacatttc tttctcctcc taaaattaaa ttctatattt taatcactgt gaattagaat
    32581 attccaatga atgaccctac caatgctaat acaactaaaa ttactaataa cttagttatt
    32641 gtccacttct tgtatttcat tccccaatat acgaagaata caacaccaag tggtaataaa
    32701 cctggagcaa tcatatttaa tgtatcttga acaacaattg ctttaccacc aatatcaaat
    32761 gatagcggag ttgaaagctt aactgtagtt gcagataatg ctcccatcat aaacattcct
    32821 agaattgaag caccatcaat tatcttattg acaataccac ctgatagaat tttcttaatt
    32881 gaatctctac ctaaagaata tcctaaatga aataagtaat aaccttcgca aaatagtaat
    32941 atactaaaca ttattgggaa aagtgcacct ataacacttc cttctgctcc aaatgaagca
    33001 cctaaagcta agaatattgt ttgaaatgta ccaaaatcta aagtatctcc aattccagcc
    33061 aaaggtccca taagaccatt cttaacacct gaaataactt ctcctgggat ttttccttca
    33121 gtagctttct cttcttccat agctaaaact gtaccgtgaa ttaatcctcc ccatatagct
    33181 tgagtattaa aaaattgtaa gtgtctcttt aacgcatcta ctaaatcttc ttttctctta
    33241 tataactttt ctaaagccgg agtgaaactt gcacaaactg ctaatgcttg cattctttca
    33301 aaagagttgg ataattctgc tgtccaccac catcttagat atgtttttgt tacatctttc
    33361 tttgtaagta ctcttgtctt attaacttca gttggacctt gtccattttt aacttcttta
    33421 ttttttattt cttgcattat acttcaccta cccttttgtc ttctttcata aaggtaatta
    33481 aaagagctat acaaacacca aatattgcag ctgcagtaat tgatatttca aaatattgaa
    33541 ccataaaaaa tcctataata aacattggta agaatttatt tttacctata gtaaatattg
    33601 ttgttgcaaa acctaatgca ggcaatatac cgcccattac tgttaatcca ttcattaacc
    33661 attgtggtat aacatctaat aatgattgaa ctaaatctgc tccaaaaaag ttacatacaa
    33721 aaactattgg aaaccttaaa acaaacccaa atattaaaga ataaagtgtt gcacatctcc
    33781 agatgccttt tgtattacct tcttcagcat acttatctgc cttatgaacc aatacagcat
    33841 taccagttct tcttaagtta ttaactaata ctcctataac acctaaaggt accgcaatag
    33901 atactgcaac ttctgcatta acaccagttt gaagtgctac tggtattgca ataagagcag
    33961 ctagacattt atctgatgga atatttcccc ctgctgcgac cattcctaga taaaccattt
    34021 ctatacttgc accaacaatc ataccagttg ttacatcacc ccaaagtaat ccaaggctaa
    34081 aacctataaa taaaggagac tttagtgttg agaaaagggt ataccctgca agacctgctg
    34141 caaaccaata ccataatcct gttacaattg ccaacatcaa aacagattcc atttataatt
    34201 cctcctaatt tatatttttt atttaaattt ctttaacgca ttttcatatg tcatagatga
    34261 ttcctctggt ataacttgta ggactatttc aaccccattt tcattaagtt cattaagttg
    34321 ttctacatcc tcatttccta atgaaacagc tgtaaacacc attttctttc ctgcttcatt
    34381 tggaatacct cctaattgaa tcttttcata tttaagtcca gctttataag ccttatttgc
    34441 catatcaaca tttttaaata gaataaatat atttttatct cctaaagtgt tgttttggaa
    34501 tgcttgtccc gcatcttctg ctgttaaaat aactacttct actcctgatg gtgctgccat
    34561 tttatatatg tcagccataa attcatcttg acctaagaag tcatcaacta ttattatttt
    34621 ttgagcttga gcgattttta cccattttgt tataacttgc ccatgaatta atctgtggtc
    34681 tactcttact aatgttattt ttgccattat attcacctca taatttatat ttttttaaat
    34741 tctttaatta aatccacaca aaggttatta ttttttttga cgattctttc tggtaatgta
    34801 tgtaatttat cttctttgct taattcttca tctacgatta aaattgtctg taagctaagt
    34861 ccatttattg catctattcc tcttcttata gcaacagcta ttgcgacact tgatgtagat
    34921 cctccatata aatctgatat tataattttc acatcttctt tatctactac ttcttcaacc
    34981 tctttcataa atacagaaac atctttttca gaacttagtg ataaaacttc atattgattt
    35041 gacaatccaa agtttttatt tgtttcttct actaagactt ttccccaagt tccatggcac
    35101 agtatgatta ccttatattt attcattgct aaataaactc tcctttatag cgattaatat
    35161 tttggtttaa ttctacttct tcttcatcag gtaccatttg aattgataca ttaatattct
    35221 ttttaataaa ttcatctatt aaaagaaact cttttttatt aatgtataca ttttgagata
    35281 ttttcttttt tcctattcct gtaggtattc ttccaacaat aagttttttg atattaatcc
    35341 cttcttctat cattctattt gcagtatcta aatttttaat taatacaaaa acgttatcat
    35401 cagtttgatt attataaaaa tatctccttg attcacttac agttaatatc gataaatcaa
    35461 tattctttgg tgctgccatt tttatcaaac ctttagcaat aacatcattg gttgtttcat
    35521 catctactac tactatcttt tttacaccta gatgcttaac ccatttatat ataacttgac
    35581 catgtattaa tctatcatca catctgcaat attttactaa agacattttt aatcaaactc
    35641 ctttttctat tacattgagc taagaacttt attaatattg tttataccag tttgtcctgc
    35701 ttcaatacat gcttctgcta actcttctaa actttttccc tctctattaa ttaaagcttc
    35761 aagtagcatt ggaagattta ctccagtaag tgcatgaaac ttgccacctt ttttaaggtt
    35821 taaacttgca acattacttg gcgaacctcc taataggtca gctaatacta aaactccgtc
    35881 accttcatct aattcattta acttttctac aaaattttta tttaagtctt caatattatc
    35941 attcctattt agtttaattg ctgctgttct ttctgctggt cctgcaataa gttcacagct
    36001 ttttataatt tcttcactaa aactcccatg agtcataact attattccta tcaccaattt
    36061 cacctcactt ttcttgtcac tttttatata agcaattgcc gtgccaactt atgtgtatca
    36121 ataacaaatg gctattttac aggatttaat caaaactttt tactcaattt tttttaaaaa
    36181 cttattattt cgatatgtgt caaaagattt tttcaacctg tatcaaatct gtatcaaaaa
    36241 aagtaaaaaa aagatagcct tttggctatt tttaaatttt gtatcaactt ttgaatttat
    36301 ctgtatcata ttgtgattca aatatatcta atatatttgc atattccata tctgaaataa
    36361 ttatatcgaa tgtctcttct actagcttaa aactttcttt tataactaaa tataattctt
    36421 tatttctact aattctttct tcatatgatt tataaactaa agactctttt attattactc
    36481 tttcaatcat acatgaacaa tgaaatataa actttactaa aagttcatct tgaattacta
    36541 aagaatagtg ttcactaatt ctatta
//
LOCUS       DAEMIK010000022        35002 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEMIK010000022 DAEMIK010000000
VERSION     DAEMIK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.022"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..1177
                     /locus_tag="KPK87_000941"
     CDS             167..1177
                     /locus_tag="KPK87_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2537115.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1313..1801
                     /locus_tag="KPK87_000942"
     CDS             1313..1801
                     /locus_tag="KPK87_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2537116.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1977..3905
                     /locus_tag="KPK87_000943"
     CDS             1977..3905
                     /locus_tag="KPK87_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2537117.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4082..4492
                     /locus_tag="KPK87_000944"
     CDS             4082..4492
                     /locus_tag="KPK87_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG2537118.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4514..4816
                     /locus_tag="KPK87_000945"
     CDS             4514..4816
                     /locus_tag="KPK87_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG2537119.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5033..5725)
                     /locus_tag="KPK87_000946"
     CDS             complement(5033..5725)
                     /locus_tag="KPK87_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG2537120.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5751..6938)
                     /locus_tag="KPK87_000947"
     CDS             complement(5751..6938)
                     /locus_tag="KPK87_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2537121.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7274..9151
                     /locus_tag="KPK87_000948"
     CDS             7274..9151
                     /locus_tag="KPK87_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2537122.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9508..10914
                     /locus_tag="KPK87_000949"
     CDS             9508..10914
                     /locus_tag="KPK87_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2537123.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10942..11790
                     /locus_tag="KPK87_000950"
     CDS             10942..11790
                     /locus_tag="KPK87_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2537124.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12451..13143
                     /locus_tag="KPK87_000951"
     CDS             12451..13143
                     /locus_tag="KPK87_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537125.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            13140..14075
                     /locus_tag="KPK87_000952"
     CDS             13140..14075
                     /locus_tag="KPK87_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2537126.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            14223..14348
                     /locus_tag="KPK87_000953"
                     /pseudo
     CDS             14223..14348
                     /locus_tag="KPK87_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(14370..16118)
                     /locus_tag="KPK87_000954"
     CDS             complement(14370..16118)
                     /locus_tag="KPK87_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2537127.1"
                     /translation="MPRVRKVKKSNSKNHMVWRIAVYIRLSKDDGNDESLSVTNQKKI
                     IREYLENFFEEEYILVDIYIDDGISGTTDDARTEFIRMIDDVKAGLVNCIICKTLART
                     FRNYADQGYYLEELFPLYNTRFICLGSPSFDTYKNPESITDGMDVPITGLMNDRYSAR
                     TSNDVRRTFNMKRRNGEFIGAFAPYGYKKDPTNKNCLIIDEVPAQVVRDIYRWYVEDG
                     MSKIGIVRHLNELGVPTPMDYKHSQGLNLKTPNQAKNDGMWGISTIARILSNRMYIGD
                     MVQGKQRVISYKVHKAIATPESEWFIVENTHEPIIDKVTFEMAQSLSQRDTRTAPTKR
                     NVYLFSGFLRCADCDKSMTKKTNKKNMANGETKEYTYYVCSTYALKNKDKCSRHSISL
                     EDLTEAVLNAIQVQISLVSNMAEVITEINKQEAVCNQSLRLTKQLQTKEHELEKITNV
                     IDNLYMDWKCGEITRAEYVRMKSKFEAKADSMKNAIVNLKAEIELSSKGVGNNDPYLQ
                     MFLKHKNITELNRGILAELIDTIYIHENNEITIKFNFADQHKRLLEFISNNQNTSKKE
                     GRVYTLPSLSKSTLAV"
     gene            complement(16184..16423)
                     /locus_tag="KPK87_000955"
     CDS             complement(16184..16423)
                     /locus_tag="KPK87_000955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537128.1"
                     /translation="MNDLGNKQTTVCSDYLEAVKSINTDKALSNTERLSACIEELNNP
                     YCFLCGKTLVKVVFNEDAPTLDFCLINYFKSLKSY"
     gene            complement(16748..16981)
                     /locus_tag="KPK87_000956"
     CDS             complement(16748..16981)
                     /locus_tag="KPK87_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2537129.1"
                     /translation="MPKELTPTFTVISKACDGDETAISKILEFYDPYINQCCMRPFYD
                     DNNNLRLAVDLEMKGFIKEAIIRKILNFDIKRF"
     gene            complement(16966..17406)
                     /locus_tag="KPK87_000957"
     CDS             complement(16966..17406)
                     /locus_tag="KPK87_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537130.1"
                     /translation="MKPSELQEKTCHQFDCAIKTVIRHRAIDFYRAIRRSEKKEMPFA
                     EIMDDLLNSIGTEDTYSTDYVAVFEVCGEKVSIEDEQLARALKAIEERKRNIVLQYFF
                     IGDTDTYIGKMLDCSRANVTKCRIQALDKLKQLIEEEKEKCQKS"
     gene            complement(17681..18316)
                     /locus_tag="KPK87_000958"
     CDS             complement(17681..18316)
                     /locus_tag="KPK87_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537131.1"
                     /translation="MLEVKNVSYAYKTKKDKTILNNVSATFEEGIFYTIVGPSGAGKT
                     TLLSLLAGLDTAVKGTVLCNGEDITKKGLNYHRKHNISLVFQNYNLIDYLTTVENVKL
                     GGSGNAEKLLEEVGIGKEYWQRNVLQLSGGQQQRVAIARALASDAHVLLADEPTGNLD
                     ETTADEIIALLKKTAHELGKCVVVVTHSKHLAEEADEILEIKNGAISFLSE"
     gene            complement(18330..19772)
                     /locus_tag="KPK87_000959"
     CDS             complement(18330..19772)
                     /locus_tag="KPK87_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537132.1"
                     /translation="MNFFGLARRSVFRKPVKSILLLLIVFAISTLLLAGVASKNASIE
                     VQDKTRQAIGAGFLLERNAEYRTERLREYSEKIGNKEGSFGGYHQKKVVVNGVENWQG
                     WSTNEFETLDIKDIEKLAKTKGIADYNLTTAITAVNPVNFKRIEDKDVDQNADVGGVS
                     LIGNKDMKLDRNVLSGNLHIKEGRMITPEDKDMCVISEELAKQNNLKIGDKISFNDYH
                     DTVNSKVSEAEIVGIYQVDQKMSPLMQGDTYRSENVIFTDLRFPEKAEGETSPLYERA
                     YFKVEDVEAYDEVKENLQKVDINWEQYDLIDNNGNSETMSSNFNDLAKVSEMMILVIS
                     VASFVILVLVFLFWLKNRVQEVGIFLSLGVPKFRIIGQIWSEAIMIAVLSLMLSFAVA
                     PAVSKVTANYLVSQQVQQMQEEEKNNEGKVSTEYVAPKQDVQSISVEVTPEMYLLDGA
                     SVLVLITASVLVSGIVILKRNPKDILSEMS"
     gene            complement(19776..20999)
                     /locus_tag="KPK87_000960"
     CDS             complement(19776..20999)
                     /locus_tag="KPK87_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537133.1"
                     /translation="MKFYQLGFRYLQRKKGKTILLLIVLILVNSMILGTSMILRTTNE
                     SKQAMQEKTNSKIVAEITSKDSKITENEVNKIETLEDVSSINRIGRQEVFPNNFAPVT
                     LNTSTNEENLKIALLSYDDLEKDSPFSEMQYRLASGDYIKHEKKGAVINTNLANQNGL
                     KVGDTIELSTENGTKVSVQIIGIFMSAGNAEKDQPSETTAVNRIENQIFIDNSTYKEL
                     FKEAEFEKICIYSKRPEKLDVLETSLQKIFGNDVALSTSDTLYQQMSAPLEQISKVAN
                     LMLVLTLVTGTVVVSLLLCMWMRTRQKEVAIFMSLGKTKIEIFLQTLLEAGSVFTLSV
                     LGATAIGKLFSGVLQNIITSSESVTENLKVVLQIQDIGMLFLLGGAVILVALCCSILP
                     ILRANPKDILAKMEG"
     gene            complement(21011..21505)
                     /locus_tag="KPK87_000961"
     CDS             complement(21011..21505)
                     /locus_tag="KPK87_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HBG2537134.1"
                     /translation="MKKTNISKFIAVGLCICALTGCGASPDEKPDTSNPIVNSNTNEE
                     NANGSSENKGNDILDSANLIGSVLEFTDNGCSVSQAKEIEGGAGIKSEAAGLENKDNA
                     VSVTYNPDCEFVVATLNKQSGVTNVTTGSISDVKKQSEVYLYGEFADTNHFNATKVVV
                     ARWE"
     gene            21700..22386
                     /locus_tag="KPK87_000962"
     CDS             21700..22386
                     /locus_tag="KPK87_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537135.1"
                     /translation="MKILLLEDDLELCKNIKISLEKENYMVDCCNDGETAMLYALNTD
                     YAYDLAIVDRMLPIIDGLTIIKSMRRKGISIPILIITAMSTLDNRVEGLDGGADDYLV
                     KPFHIKELLARVRALTRRPANIQLSPKLQYGDLTFDKDSRTLSTPVNSILLTSKETEL
                     TFVFMQNPETLFSREQLILKIWGSSSEVETGNVDSYISFLRKRLKELKSSCKITTVYG
                     AGYKLEKETC"
     gene            22380..23672
                     /locus_tag="KPK87_000963"
     CDS             22380..23672
                     /locus_tag="KPK87_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2537136.1"
                     /translation="MLNNLYRKLHILFASSVMLIITLVISFVVANTVHTEKINESTLF
                     QRLTTLLIYQVESASSDMDKALKTYEESYHIFSLITDTRGNTIYQSDFPFPTSADNLL
                     HDVEMQISTQPLSQTESNTTSQGGFLEIRGTHHDTYFVIPATIRTANDTVYHATFLYQ
                     TASLTDILQKTLPIYLLIWFLACIVVIVLTRYLLKKSFAPTERILQSQKDFVATASHE
                     LKSPLAVMISNTDMLLDNVSLNEQAKQAVQTVDFECMRLSRLVKDMLLLASSDAKTWT
                     LHKSTINVDTLLITLYETYEPVCMKQNLKLKLHLSEESYPAMLTDKDRLFQILCVFMD
                     NAIQHSKNNSLIEIEVIATEKNIAFSVVDHGQGISDEDKQYIFDRFYSGDKSHTNKSN
                     FGLGLSIAKELTQMLNGAITINDTAGGGATFTVKFPLK"
     gene            complement(23718..23942)
                     /locus_tag="KPK87_000964"
     CDS             complement(23718..23942)
                     /locus_tag="KPK87_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537137.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTAP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTKI"
     gene            complement(23988..24683)
                     /locus_tag="KPK87_000965"
     CDS             complement(23988..24683)
                     /locus_tag="KPK87_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537138.1"
                     /translation="MCTRFVYNGIDKIVGFNFDIDLSVWDHTVITEENRFYIGIKMPD
                     GMYHSFHGINKNGNVGTLLYVHGNENARYIESEDCITISELTEKFIKAEISFDKALDI
                     VKKKKIIYAPDATMQAMLSDKKGRVLIIEPGIGYRYEQENFSLITNYSILKPDITKPY
                     IVSGDTRYEVAKELLAGYDKDFSVNDAFKVLKSVSQKDLWATRVSFVYSTEKNKVYYV
                     LNNDFSNVFEFQF"
     gene            24861..25418
                     /locus_tag="KPK87_000966"
     CDS             24861..25418
                     /locus_tag="KPK87_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2537139.1"
                     /translation="MKTTKEAVIKATSDLADEKGLNNVSLKAVAQKLNIRTPSLYNHI
                     ESLDNLLLEVAHNGMRDMNERMMKIAVGKIGKEAIKLVSIEYLNYMIEHPGVYETIQW
                     AVWHGTEETATIFNNYLSLLTTLIQSCSLNKDKTLEILNMLTGIIHGYTTLQLGNAFS
                     ASDKVRFELAEAIDTLLVGIFQKYK"
     gene            complement(25465..26376)
                     /locus_tag="KPK87_000967"
     CDS             complement(25465..26376)
                     /locus_tag="KPK87_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537140.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIEARTQA
                     INGYLTKELQDLNVDTIRMDIPTSSTVTDVIVWSIEQSGTDTFSATYEVDQQIKEGEQ
                     ARNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVSGNVIEPIGRDYLYSELVNPIFTKDGDNVKVKVDVKF
                     LDNQTKATQVSQYELALHKDSNWKIVG"
     gene            complement(26392..27399)
                     /locus_tag="KPK87_000968"
     CDS             complement(26392..27399)
                     /locus_tag="KPK87_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2537141.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYAELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            complement(27396..29591)
                     /locus_tag="KPK87_000969"
     CDS             complement(27396..29591)
                     /locus_tag="KPK87_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2537142.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFGSTGFYVGSLLLIIL
                     IIGIYVAYTGLIKRETSKAVQAVINFLVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEKIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLFIFNIGITIFVFLLT
                     GIMMLSQILFIIFAMFLPISFLLSMIPSQENLAKQAIVKVFNTIMTRAGITLIVTVAF
                     SISSMFYNISKEYPFFMVAFLQIVCFAGIFIKLGDLMNMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMERRLAGAVTAGGVAGAVAGNTISNRKPTRNTATKDSRGNTTSSMGQR
                     AGSKVGAVLDTRNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKRGMVQE
                     QQSRQTGRLEKQEQHKKNIADKRMELQKAQEARQVQRKADGSATTGATRPHERPATAS
                     IIPKPSAEKTQEVKRPATATTSKASEPVKTNVIKERPLSSGASDRKATQSAQPVHRQN
                     VEKVVSQETRQNYTKDRRTKVHQTQSVQKNQQTTEKTRNLVMKKGQKKK"
     gene            complement(29591..32041)
                     /locus_tag="KPK87_000970"
     CDS             complement(29591..32041)
                     /locus_tag="KPK87_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2537143.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIVQSREGKIHALQIATESSIRSQQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIMENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPAGKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTLISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVAKESLKNNIGLK
                     FAFRSTDLGEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(32019..32417)
                     /locus_tag="KPK87_000971"
     CDS             complement(32019..32417)
                     /locus_tag="KPK87_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537144.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            complement(32527..33030)
                     /locus_tag="KPK87_000972"
     CDS             complement(32527..33030)
                     /locus_tag="KPK87_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2537145.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISYYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            complement(33048..33551)
                     /locus_tag="KPK87_000973"
     CDS             complement(33048..33551)
                     /locus_tag="KPK87_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2537146.1"
                     /translation="MQETRVLVETRGTTGEETKSYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPFADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVAKQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(33470..33766)
                     /locus_tag="KPK87_000974"
     CDS             complement(33470..33766)
                     /locus_tag="KPK87_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537147.1"
                     /translation="MNDIFKDMQANVGCEYISDLPSYKRKVWHEMKRLNPADYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKRNHTGLTCR"
     gene            complement(33858..34166)
                     /locus_tag="KPK87_000975"
     CDS             complement(33858..34166)
                     /locus_tag="KPK87_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537148.1"
                     /translation="MKKLYCMNCGAQIKGGIPNKCSCGAEFDLADRKNIKKIITSIIF
                     FVILMSPLLILIHFGRQYLQASIILYALLLILGVFWYRIVQTILVRLGLVTMTNIEIK
                     "
     gene            complement(34407..34736)
                     /locus_tag="KPK87_000976"
     CDS             complement(34407..34736)
                     /locus_tag="KPK87_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG2537149.1"
                     /translation="MKLVGSRKLTWGICGIGILLALVGVLFLPQIIPVHFANGIADDF
                     GNKIEIFLFPILLLIITILTGKEKIKYFLTHSKTFLTDIQYNLMIDGVLGIVLIAEIY
                     VIYASFV"
     gene            complement(34823..34984)
                     /locus_tag="KPK87_000977"
     CDS             complement(34823..34984)
                     /locus_tag="KPK87_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537150.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALIAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
BASE COUNT        11207 a         6099 c         6193 g        11503 t
ORIGIN      
        1 attttgggta ttgtatactg gaattagagt aattaattta ttttttaaca tacaaatgat
       61 tttatgaaaa aaaacaagac tcaaaaattt ttatattctt tgatagaata aaattgagag
      121 taaatttatt gaattttata aaatttataa ggggtgagtc taagatatgc atgaagataa
      181 aaatgatata tttgaatcaa tagatactga ggattttgat aataaaacag aagaagaatg
      241 tttagagcta gtaagaaaaa atggattgaa tttaatatac ataaaaaatc aaacagaaga
      301 gatatgtcta gaggctgtta gacaaaattg taatgcaata aaatgtgttc agaatcagac
      361 agaaaaaata tgtatagaag cagttaaaca agattggaga atgttagaat ttgtggaaga
      421 gccaacagaa gaaatttgca tgttggctat taaccaagat ggaacagcat taaaatatat
      481 agaaaatcag acagaggagt tatgcttaag agccgttgaa aaaaatggtg cggctttgga
      541 gtatgtaaaa gaacaaacag aagaaatatg tatagaagca gttagaaaca gcgaatttgg
      601 attggcaaga gtgaaaaatc aaacagaaaa aatatgtatg gaagcagtta agcattgtag
      661 ctataatcta aaatgggtag aaaatcaaac agaagaaata tgtatggaag cagtgagaca
      721 aaatgggctt gatttgaaat ttgtaaaaaa tcaaacagaa acaatctgtt taagagcagt
      781 gagacaaaat ggaatggcat tagaatttgt aaaggaacag actgttggta tatgcttaaa
      841 ggcagtaaga caatatggaa tggcattaaa atttgtaaaa gaacaaacag aagaaatttg
      901 tacagaagct ataaaacaag ataaaagagc acttagtttt gtaaaaggag ataaggaaaa
      961 atacaaggct ctatatgata ataatgaacc atttgcaaag agatatgtta gaaatgtaat
     1021 agaaaaagaa aacagagcat tgattaaaaa aggagaagaa aatgcaaaaa tcaaaattgc
     1081 aggaaaactt tatgatagag gcatgtcttt tgaagatata tcagatatag ttgagattga
     1141 aataagcaag ttaaaagtga gtttaggtgt tttttaaagt aataaatgta ggtgtttttt
     1201 atgggaaata gttaataaaa aaataatcga ctagctcttt gtaggacagc aaaattagtg
     1261 atataatttg atgtataata aattattttt attagataag ggggaattaa agttgaagat
     1321 tacagtatta taccaaacaa gaagtggaaa cacagaaagg gtcgctaagc tgatagaaga
     1381 gggcgctaaa aaggtggatg gagtagatgt aaagcttatg agacttgata atattgattt
     1441 agattattta aatgaaagta aagcagtaat atttggaaca cctacttatt tagcaaatac
     1501 aacttgggaa gtaaaaaaat ggtttgatga agattcaaaa aaagttaatt tagctggaaa
     1561 attaggagct gtttttgcta catgtgatta tatatgtgga ggtccagatg ttgcgatact
     1621 tactatagta ggacatttga tggttaaagg tatgcttgtg tattctggag gtggttcttt
     1681 aggaaagcca tttatacatt taggacatgt acattcaatt gaaggtccag agttacaaga
     1741 tgagaaggct ataatatttg gtgaaagaat tgctactaag gcaaaagaac tatttgctta
     1801 atttctatat gcataataat gattagagta aatttagggt tttaagggag atttgaagta
     1861 aaagttataa aataaaaaat tttccataaa attactcaag atgaattata atataattat
     1921 aaataaatta tattattaaa atatttaaaa tcaaacgtca atagcgaggg aagtttatgg
     1981 tatggcatat agaatatgag attttttctg caattatagt tattttttta atggtatact
     2041 tttttagaag taaatttata cctactttac aaaacaaaat atattgtgca cttttaatat
     2101 tctcattttt atttataatt tcaaatattt taggttcatt ttgtctaaat aatatagaca
     2161 aaattccaat ttttattact tttttattga atcagatata cttattgtta ttgccaatac
     2221 cagcagcact tgtatcattt tatgttatgg ctattattta tcaagatata aggtatatga
     2281 aaaaaagatt attattttta tctataccac ttataatttg tataatctta tctattacaa
     2341 atccttttac gcatattcta tttggattgt caaaagaaac aggttatata cgaggttgtg
     2401 gatatgttgc aacttttgca tcttttttct tttatgcaat atattcagca tttttatctt
     2461 ttaaatatag aaaagcaatg catgaatcaa aaatatgggc tattagaggt tttcttatag
     2521 tttcaattgt ggcaattatc ttacaggcaa tttttttaca gtatcttctt acaggaactg
     2581 catgtgcatg tagtttactt ttgacatatc tttctatgca gaatcgaggt cttattattg
     2641 atgaccttac tggtgtactc aatagacaat cttttattca agagctagat ttgaatatca
     2701 atactgaaca atcaggattt attattacaa ttgcgcttga tgattttaag tttatgaatg
     2761 aaacttttgg aacaaaaaat ggagatattg cactaaaaga agttggaaaa tatttgattc
     2821 agatacatga tgtaaaccat gtatatagaa tagggggaga tatattttct attgttgtca
     2881 atagtcaatt aggtatagaa ccagatgata ttatcagaaa gatagaagaa agatttaaaa
     2941 agccttggct agtagaagag ataagtttta atttagcaac tagtattgct gttgtttatt
     3001 acccagaaaa tgcagaaacg acagaagatg taattgttgc tatagatttc tctatacatg
     3061 aagcaaaacg ttctgagtca aggcgagttg tttatgcaga tgcgtctata agtgaaaaga
     3121 taaagaagaa acatgttatt aaaaattgtt taaaagaagc attaaaaaat gatgggtttg
     3181 aagtgtacta ccaacctata ttttctaata gcaggggcag gtttacttca gcagaggcat
     3241 tacttaggct agaacacaaa gagttaggtt ttatacctcc tgatgaattt attccaattg
     3301 cagaacaaac aggattgatt aactcaattg gattagtagt ctttgagaag gtatgtagat
     3361 ttatagctag tgatgagttt gaaaatttaa aattggataa tatagctgtg aatctttctg
     3421 tagtacaatg tatgcaaaaa aacttagctg atgatttact ttatcttatg aataaatata
     3481 atgtatcacc aactaaattt aaacttgaaa ttactgagac tgtggctatg ggctctttta
     3541 atataataaa agaaacaatg gaaagattaa ttgatttagg tgttaagttt gcacttgatg
     3601 attttggaat tgcttattct ggagttacta atatgctttc tttaccattt tcagtaatta
     3661 agttagataa gagtttgatt tggtctatga atgaagatag taggcataaa cttacagttg
     3721 agacgattat agcacttata aataaattga atatgaaggc tgtagcagaa ggtgttgaaa
     3781 ccattaagtg tgctgaagat ttaatagcta tggagtgtga atatttgcaa gggtattatt
     3841 tttctaaacc agttccagaa gatgttttta aaaatttgtt gaaagaaaat acttttacaa
     3901 gttaacaccc tttatttttt taattacaac tttgttcatt gacactgcca gcgatatatt
     3961 ataaaattaa cacaataata aatattaaat ttattataca tattattaat attaattaat
     4021 ggagtaaaga tttattatat attttattta attatcctaa agtgagaaag gaagatgtga
     4081 catgaataaa aagaaaatat tagtgccaat tgatgggaca gaaagaagta tgcattcttt
     4141 ggaatttata aaaggaatat ttaaaaaaga tgaagttgaa gtagaaataa tgaatgtaaa
     4201 agaactagta tttattgatg gcatatccct ggctgaagag ataaaaaatt ctgaaaattt
     4261 aggaagaaga atcttggata aagcagcaga gataatggga gagtatgatg ttaaagtaca
     4321 ttttactttt ggatatccag gagatgagat aattagaaaa gcaaaagaag ataatgtaga
     4381 ttttatagtt atgacaaaat ctactaaaaa aggtcttaca agaatgatag gttcagtaac
     4441 agcaagtgta gttaaacaag caaaatgtat agtgatgatt gtaccggagt agaattagaa
     4501 aggtatttga aatatgaata aaaagatagt agcagtttgt gaaagtgaaa gaagtttaaa
     4561 ctctcttaag acagctgcaa atgatttagg atttgattta gtttatgaaa ttcaagaaaa
     4621 tgataatata aaaaatgaac tttctattta tgatatagaa gatgcatcca tagtgttatt
     4681 tgttacaagt gattctatag aaagcataga gaagattgat agatttatag atagagaatt
     4741 ttatgaagtt gatccaaagt atataataga ggatgcaaaa tctatagtaa gtgaaatttt
     4801 aatagactta aattaatcgt gggtgtcgca ctaagaatag agcgacgcct ttttatatat
     4861 tttacacaag aaataaagag gttgtatcaa aatatagata agactctttt tcattgttga
     4921 aatgatgaaa atatatttga tttcatacaa aaaagagtac ctcataacta aaaagttcac
     4981 tttgagacac tccattttta tatgttagtt gcatttaagg taaaatataa gtttaatcat
     5041 gttgaaataa ctgaattaca tcttctaaat gattgtacat tgatgtataa cctaagtaag
     5101 agtctttact atcaaatgca atcattatct tgttatggtg gctaatcata tccttgagag
     5161 aatttctata tttaaaaccg aagtattggt tagaagaaac tgttttaggt attaactcat
     5221 aatattttat aaaaattgga ttatgagaag ctaaaattat ctctttgtga aattgcaagt
     5281 cagcatcata acattcttct atcaaaccat gagaaacctt atccaattcc tctattgact
     5341 ttaggtaagt atattttatt ttttgaagtt ctttagatgt agcatttaaa attgctagtt
     5401 tagtagcttc actttcgata agttgtcttg ctcttaaaga ttctacataa aattcttgta
     5461 tgttatgatt tttatagatt tcaccatcat caaataggtc aactttttta aatccttcaa
     5521 ctataggact tgaataaggc acagctctaa gtattccttc cttacagagt atatttatgg
     5581 catttcttac aggtattcta cttatgttaa ataggtcagc aatctttctc tcacttggta
     5641 atttactatt aattttaaaa gttccattta gaatgtttaa tttaatataa tcaataattt
     5701 tttcagtggc atttaagctt ttcataaaaa ctcctaaaca tataattttt ttatgattgt
     5761 attatatcat agttaagagt taaatctact tttttataat atgtataaga caggatagag
     5821 gccaaaatag ctataatagc caagataaag tatccatatt gtattgaagt atattccgat
     5881 attttaccaa ctatcattgg aaatacagac attcctatag cataaattgc ttgaaaaaaa
     5941 cccattgcag tagatttctg atgttttggt acatttttca tagactcgct agtaaggtaa
     6001 gaaaggagta tacctgtaga tatacctggt agtacttgta gtacaaatat tatattaata
     6061 ttttttattg ttggaactaa aaagcaatag gctgaaacta aaataaaaac taaaggaatc
     6121 caaaattttg gtccatactt agtacaaaat ttagtagaag caaagccaga agaagcaaca
     6181 gcacacacca tgtaaaatat agaagataga cctatgaggg tagaactaga acctaagttt
     6241 ttaagaattt gagtagtaaa tgacattgtt gtagacattt gaataccctg ttgaattaat
     6301 gccataaagg aaaaaaatac aagtctttta tccttgcaga tagataaata ttttttaatt
     6361 gatatttttt caggtatttt ttttgaatta tgatttttta tatttaaggc tagaatcgtt
     6421 ccaataactg aagacaatac actcatgata cataaggctg tcattcctaa tttttcataa
     6481 aataaagtac tcataacaaa tccaatcaac attcctagat tgtttaccaa tattatcgta
     6541 cttgtagctt tttgttgatg ttctgtagaa aaaaagttgg tatagtgaac catgtaagaa
     6601 atccaagttg atgatgatat tccagataaa atgttagcta taagaaaacc attgcctcca
     6661 ggagaaaaga ctcttataag agatgctaaa ccagcaaaga aaattccaga cagtataaat
     6721 attttatgtt tatttataga atcagcaaat actccaacag gaagccttaa taaacattgt
     6781 gatattccat atgcacctat tattatacca atcatatttg tagatacatt acatgcactt
     6841 aaatatggat tttggaaagg tatatataca tattgtgaaa accagaaaaa tgtaacaata
     6901 gaaagtaaaa gtaatttttg attagatatt tttttcatat tatcctccat atggtatttt
     6961 tataatgcaa ataatatacc atttagattt tttaatcaag tactttctct attaaaaaaa
     7021 atttaaatat ataattatta tttacaaaaa aattatactt tatagttgaa ttaagaaaac
     7081 gcttatgata ctataaagtt aacaaatttg aatatataac agtagacgtg ttactggtaa
     7141 tgcaggcaag atcgaatcaa tttttataga ttatctatag agtactatgg gtataatcta
     7201 tttatttggt tcgatttttt tgtttaattg gccataaaac aaaaagccta tagttgaaaa
     7261 tggaggattt tttatggatt ataaaaatat agctcaagaa attctactaa atgtaggagg
     7321 aaaagagaat gtaaatgaag tgactcattg tatgacaaga cttagattta aagtcaaaag
     7381 cgcttctaaa gtgaataagg ataaattatc taaaactgaa ggagtaataa ctgttgttga
     7441 gagtatgggt caaatacaag ttgtaattgg aaataaagta aaaaaagtat atgatgaggt
     7501 tataaaaatt gtacctcaat caaataacat aggtaaaaag aatgaaagtc aagaaaagca
     7561 aggtataatt aattctatat tatcagcagt agctggtata tttacaccta caatacctgc
     7621 tatagcaggg gttggtatga ttaaaggtat tttatcagtt cttgctatgt attatatgaa
     7681 taaaaatgga gtagatataa aagaaacgca aagctatatt atcttaaatg ccatggctga
     7741 ttctatattc tattttatgc ctattatact aggatatact gcggctaaag tatttaatgc
     7801 aaataaaata atatctatgg tattaggagc tacactttgt tatccaactt ttactgcttt
     7861 gatggcaggt gaagaatctg ttagattttt aggattggca gtaacaaaag ctacgtatac
     7921 atcatctgtt attccaataa ttattgctat atgggctctt tcatacgttg aaaaagtatt
     7981 agaaaagtat atacctgagg taattaaaat aataatggtt ccaactttat cattggttgt
     8041 aatgttacct gcaacattat ttttatttgg accaatcgga atttatatag gaaatgtcat
     8101 aaacttttct tataagtata tttatgaatt aagcccagca ttgtgtggag catttgtagg
     8161 tgggttatgg tgtgtgttgg ttatatttgg tgctcataga gcattattac caataggtat
     8221 aagtgatgtt gctcaaacag gaagacagaa tttattggca tttgcaggag ctgcaaactt
     8281 ttcacaagca ggggctgctc taggagtatt ctttaaaact aaaaatcaag gactaaaaac
     8341 aatttctatg tctgcaacta taacagcttt atttggtata actgaacctg caatatacgg
     8401 tgccaatctt agattaaaaa aacctatggt atgtgccgta atatgtggtg catttggagg
     8461 tgcgataatg ggaatgggtg gagcttatgg taatgcattt gcaaaccaag gtgttttaac
     8521 tatacctgta tatgctgaag caggtgcatt aggtttctta agttatctag gtggttgtgc
     8581 tatagcattt tttggttcag ctatttcaac ttatctagtt ggatttgaag atttggaaga
     8641 gtcttctaat aaggaaaatt catctataaa agtagaaacg aaggatggtg ttattgatat
     8701 aacttcacct gtagagggcg aatgtataga attgtcagaa gtaaaggatg atgtatttgc
     8761 ttcaaaagct atgggagaag gtatagctgt gttacctaca aaaggagtaa taacagcccc
     8821 tactgattgt gaagtagctt ctttatttcc aacattacat gcaataggat taaaattaga
     8881 taatggagca gaaatgttaa tacatgttgg aatcaataca gtagagttaa atggtaaata
     8941 ctttacaaaa catgttaatc aaggagattt agtgaaaaaa ggtgataaac ttatatcgtt
     9001 tgatatagat aaaataaaaa aggctggtta tgatgtaact acgccagtta tagtaaacaa
     9061 tacatttgat tttggacaag tagtttcttg taaatctagt tatgtttcaa ccaatgataa
     9121 tataatttca ttagtattag ctggtaatta agttaatttg actagagata agtaaattga
     9181 tttaaaagga aataagtaaa tcaacaatta aatacatcta aaagtaagtt tattgcaatg
     9241 aattgttaaa gaaaataatt aatatggagt aaagctattg caatgaagta acaaagaaaa
     9301 taattaatat agagtaagtt tattgtaatg aattgttaaa gaaaataatt aatatagagt
     9361 aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc tatctcaatg
     9421 aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca acaaagaaaa
     9481 taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga ctttttatgg
     9541 ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa taaaggaaaa
     9601 accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac taaagtcgca
     9661 tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt agggatgaaa
     9721 acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg agagataaac
     9781 cagaaaggtt tagaatttta taataagata atagacaaac taatagagtg tgatattgag
     9841 ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa atataatggt
     9901 tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg ctttaagcat
     9961 tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat tgttagagtg
    10021 gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa aatgcgtgct
    10081 cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg tcatgagatg
    10141 ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc tcttacttct
    10201 aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta ctatatgctt
    10261 gatatgcatc attatggaga atatccaggg tattatatga agtatcttga aaaaagaaat
    10321 atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa aatggatttt
    10381 atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga ttcacaacat
    10441 ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt taaaatgtca
    10501 atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc aggtcttaga
    10561 gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac agaaaatggt
    10621 ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg tatagactat
    10681 ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga gatgattgga
    10741 tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa taagcgttat
    10801 ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag aatcaagaaa
    10861 gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa ataaacataa
    10921 atatatattg agggttttag tatgtacatt acaaaggtat taaataattc gttgctactt
    10981 gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg ccataattat
    11041 aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct tcatgatgaa
    11101 aatataaaaa agagtattat acaattggca acagaaatag atgcagaata ttttggtata
    11161 gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat gaaccatata
    11221 tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga aggtataatg
    11281 attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta tgatatagga
    11341 aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga agaagagatt
    11401 ggtaatatag caggccattt tataaatgca caacaagata atccttatag tgatagaaat
    11461 aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca cttttctatt
    11521 gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa ggccttttca
    11581 caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata tgagcaagtt
    11641 caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta tatgtataag
    11701 gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca tattcataag
    11761 attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta atattataaa
    11821 aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg gggttgtctt
    11881 aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct ttatatcatt
    11941 acaactttaa tgtttatacg actgtacttt agatgtatat ctacttttta catttctcaa
    12001 catgcaaacc taccattttt tgcagtatgt taattgaaat aaaccattga gttaacccta
    12061 aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt tctggaataa
    12121 ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct tcaactctgt
    12181 atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata cgcttgattt
    12241 tttcacaatg atgaaatata ctaatgggtg ttataggtat aagaatcgca ttacacttga
    12301 agaagtaagt gataaatgag atataaatcc tcatatgata aactactatt tcattgagga
    12361 acatattcta agtgatgtca agatactaca atatgactaa attcctagtg ataaaaaagt
    12421 ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat agatgatgat
    12481 aaagatcttt gtattttgtt aaaaaagagt atttcaattg aacagattga agcaaactat
    12541 tgccattctg ggagagatgg actggttgaa ctttcaaaac aagattatca gttagtagtt
    12601 ctagatgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat acgaaaatat
    12661 agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa agtgcatgga
    12721 ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga atttactgct
    12781 cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga ggagccacag
    12841 caactttctt tcaaaggttt gttgattgac cttgaccagc atacagttac taccaatggc
    12901 gaacctatgt tgcttccacc caaggaattt ggtgtacttc tttactgtgc aaggcatcag
    12961 ggtaagattt tgacaaaaca acagatttat gaagccgtgt ggggtgagcc atatgtatat
    13021 gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga actagaccca
    13081 tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa cacggaggta
    13141 tgaaaatgga tgaacaaacg ataattcttt tcttactctt gctgtcgatt ggtctacttt
    13201 ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta aaagatatag
    13261 aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta attaagccat
    13321 gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat tatgaaaata
    13381 agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca agtttatctc
    13441 atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta cacagtggta
    13501 tagttatcga acaagagcgg gaggaatacc ttgaaatcgc taggcgtaga gcctacgacc
    13561 tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat gaatttacgc
    13621 tgtcaataga atctgtggaa attgcagagt tgtcacgcaa tattttgaaa gactggattc
    13681 ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat cgactaatgg
    13741 taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa aatgttcttg
    13801 ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga atggtattac
    13861 tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat atttttgagc
    13921 gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt ctatctattg
    13981 tcagtcaaat ggtggagcga atgggtggtc aggtttccgt agaaagtgag attggaaagt
    14041 ataccgtatt tacagtgagc ttaccattaa tataaaattg tttttccccc atgtgacatg
    14101 tatacacatg gggatttttt gtaagttata aagttgcaag gttaatacaa ggttgttgca
    14161 aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg cttttattat
    14221 ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt gctcaaaaga
    14281 gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg ctaggaagaa
    14341 acggtgcata gatatataga caaaaattgc taaacagcta atgtagattt agatagtgag
    14401 gggagtgtat aaacacgtcc ctctttttta gatgtgttct gattgtttga gataaactct
    14461 aacaatcttt tatgctggtc tgcaaaatta aacttaattg taatttcgtt gttttcgtgg
    14521 atatagattg tatcaatcaa ttcagctaat attccacggt ttaactcggt tatgttctta
    14581 tgtttcaaga acatttgtag gtaagggtca ttattgccta cgccttttga ggaaagctca
    14641 atctcggctt taagattgac aatcgcattt ttcatgctgt ctgcctttgc ttcaaatttg
    14701 gatttcattc ttacatattc agctctggta atctcgccac acttccagtc catatacaaa
    14761 ttgtctatga cattagtaat cttttcaagc tcatgttctt tggtttgtaa ttgctttgtc
    14821 aatcgcaagg actgattgca tacagcttct tgcttattga tttctgtgat tacctctgcc
    14881 atgttactaa caagagaaat ttgtacttga attgcattga gtacggcttc tgtcaaatct
    14941 tctaatgaaa tgctgtgacg gctacactta tctttatttt ttaaagcata agtagaacac
    15001 acataataag tatattcttt ggtttctcca tttgccatgt tctttttgtt ggttttcttt
    15061 gtcatgctct tatcacaatc tgcacaacgt aggaaaccag aaaacagata gacatttctt
    15121 tttgtaggag cagtacgggt atcacgttga gataaagact gtgccatttc aaaggttact
    15181 ttgtcaataa taggttcgtg agtattctct acgataaacc attcactctc tggcgttgct
    15241 atcgctttat gaaccttata gctgattact cgctgtttcc cttgtaccat atccccgata
    15301 tacattcgat tacttaaaat tcttgctatc gttgaaatgc cccacatacc gtcattcttt
    15361 gcttgattag gtgttttgag gttcaagcct tgactgtgct tgtagtccat aggtgtaggt
    15421 acgcctaatt cgttcaagtg tcttacaata ccgattttac tcataccgtc ctctacatac
    15481 cagcggtaaa tatcccgtac cacttgtgca gggacttcat caatgataag gcaattttta
    15541 tttgttgggt ctttcttata tccataagga gcgaaagccc cgataaactc gccattacga
    15601 cgtttcatat taaaggtacg tcgaacatca ttagaagttc ttgctgaata acggtcattc
    15661 atcaatcccg taatcggaac gtccatacca tctgtaatcg actctggatt tttgtaagtg
    15721 tcaaatgacg gacttcccag acagataaat cttgtgttat aaagtggaaa aagttcttcc
    15781 aaataataac cttgatctgc ataatttctg aatgttcgtg ctaatgtctt gcaaataata
    15841 caattcacaa gaccagcttt cacatcatca atcatgcgga taaactcagt tcttgcgtca
    15901 tcagttgtac cgctgatacc gtcgtcgata taaatatcca caaggatata ttcttcttca
    15961 aagaaatttt ccaagtattc acggataatc tttttctggt tcgtaacact taaactttcg
    16021 tcgttgccgt cgtcctttga taatcggata tatacggcaa ttctccatac catgtgattt
    16081 ttgctatttg attttttaac ttttctaact ctcggcatat tgatttctcc tattagaaaa
    16141 gtcatttatc acttttttat tataccatat ttcgcccatt ttttcagtaa ctttttaggc
    16201 tcttgaagta atttatcaag caaaaatcaa gcgttggagc gtcctcgtta aacacgactt
    16261 ttacaagtgt tttaccacat aagaagcagt agggattgtt taattcctct atgcaggcag
    16321 ataaacgttc tgtgttggaa agagccttgt cggtattgat tgacttgacc gcttctaaat
    16381 aatcggaaca tactgttgtc tgcttatttc ctaaatcatt caatagatat accccctttg
    16441 ctttgctgtt tagcagtttc tacttatata caagcaggca gacggctttg gaaagctccg
    16501 ttagtatctc aactattccc attcttgtgg gcagaaccgc accatgcgga cgtatcatta
    16561 ttctgtgagg ataggtcgtg gcaaagcgac ttttccaacg gtcgctctcg gatcactgcg
    16621 tgggtcgctc gctttctttt ggaaaggtca tggcgtacag tccccgtggc ttgctatctc
    16681 acaaacgtat ctgtctgctt tattcagttg tcaaagacca gacgggcata atcaatcccg
    16741 atatgaatta gaagcgttta atatcaaagt ttaagatttt acgaataatc gcttctttga
    16801 taaaaccttt catttccaaa tctaccgcaa gacgtaagtt gttgttatcg tcgtaaaacg
    16861 gacgcataca gcactgattg atatatgggt cataaaattc caagattttt gaaattgctg
    16921 tttcgtcgcc gtcgcaggct ttggaaataa ccgtgaaagt cggtgtcagc tcttttggca
    16981 tttttctttt tcctcctcaa tcagttgctt taacttgtct agtgcttgta ttctgcactt
    17041 cgttacattc gctctggaac agtcaagcat tttcccaatg taagtgtccg tgtccccaat
    17101 gaaaaaatac tgtaaaacga tattacgctt tcgttcttca atagctttca atgcacgagc
    17161 cagttgttca tcttcgattg atactttttc tccacagact tcaaatactg caacataatc
    17221 cgtagaatat gtatcttctg tacctatgga atttaagagg tcgtccataa tttcggcgaa
    17281 tggcatttct tttttctctg aacgacgtat cgctcgatag aagtcaatcg ctctatgacg
    17341 tataactgtt ttaatagcac aatcgaattg atgacaggtt ttttcttgta gttctgacgg
    17401 tttcattttc ctcacctcct ttcgctagtc gaaaagaggt gtctgcccct tttcatatat
    17461 accgcgttga aagtggggta cagcgtaacc aaaatcaaaa aaaacttaaa aaattttttt
    17521 ctggcatgaa aaaagcccgc acaaaacata tagtctgtac gagcctttga aactgtattg
    17581 tatttagttc ttataacaat gccgttaggg tcaaattgat tgtgtcgagg tatatctaaa
    17641 atattccata atgacagctc ctttacggca ctattcttgt ttattcagac agaaaagaaa
    17701 ttgcaccgtt tttaatttct aagatttcgt cggcttcttc tgccaaatgc ttactgtgtg
    17761 taaccacaac aacacatttt cctaattcat gtgcagtctt tttcagcaaa gcaatgattt
    17821 catcagcggt agtttcatca agatttcccg tcggttcatc tgccaaaagt acatgagcgt
    17881 cgctggctaa agctcttgca atcgctacac gctgttgttg tccaccagat agttgcaata
    17941 catttctctg ccagtattct tttccaatgc cgacttcttc caataatttt tcagcatttc
    18001 cactaccacc taatttgaca ttttcaaccg ttgtcaaata atcaatcaga ttataatttt
    18061 gaaataccaa tgagatatta tgctttctgt ggtaattcag cccttttttt gtaatgtcct
    18121 cgccattgca aaggacagtc ccttttaccg ctgtgtctaa gcctgctaac aacgataaga
    18181 gtgttgtttt tcctgcacca ctcggaccga caatggtata gaagataccc tcctcgaaag
    18241 tagcagatac attattcaaa atggttttat ctttttttgt tttgtaagca tagcttacat
    18301 tctttacttc taacattttc gtcgtcctcc taactcattt ccgataaaat atctttcggg
    18361 tttctcttta gtattacaat tccagatacc agaacagaag ccgttatcaa tacaagaaca
    18421 cttgctccgt ccagtagata catttctggt gtaacctcta cgctgatact ctgcacgtcc
    18481 tgctttggtg caacgtattc tgtggatact tttccctcat tattcttttc ttcctcctgc
    18541 atttgctgaa cctgctgtga taccagataa tttgcagtta ctttggaaac ggcaggagcg
    18601 acagcaaagg ataacataag ggaaagaaca gcaatcataa tcgcttcgct ccaaatttgt
    18661 ccgataattc taaatttcgg aactccgaga gataagaaaa taccgacttc ctgcacccga
    18721 tttttcagcc agaaaaggaa tactaaaaca aggataacaa aacttgcaac agagattacc
    18781 aatatcatca tttcgcttac ttttgctaaa tcattgaagt ttgaggacat ggtttcggaa
    18841 tttccgttat tgtcaatcaa atcatattgc tcccagttga tgtctacttt ttgcagattt
    18901 tcttttactt cgtcgtaagc ttccacatct tctactttga aataggctct ttcatataaa
    18961 ggacttgttt caccctctgc cttttctgga aatctcaaat ctgtaaatat tacattttcc
    19021 gaacggtaag tatcgccctg cataagtggg gacatctttt gatccacttg ataaattcca
    19081 acaatctcag cttctgatac ctttgaattt acagtatcgt ggtaatcatt aaaagaaatc
    19141 ttatcgccga ttttcagatt attttgcttt gcaagttcct ctgaaatgac acacatatct
    19201 ttatcctctg gggttatcat tcgtccctct ttgatatgca ggtttccaga taaaacattt
    19261 ctgtctagtt tcatatcttt gtttccaatt aaacttacac cgccaacatc tgcattctgg
    19321 tctacatctt tatcttcaat ccttttgaaa tttacagggt ttacggctgt gattgctgtt
    19381 gtaagattgt aatcagcaat tccttttgtt tttgccagtt tctcaatatc ctttatatct
    19441 agggtttcaa attcatttgt tgaccacccc tgccaatttt ctacaccatt aacgactact
    19501 ttcttttgat gataccctcc aaaactacct tccttattac caatcttttc tgagtattca
    19561 cgaagtcgtt ctgttcggta ctctgcattt ctttctaaaa gaaagcctgc acctatggct
    19621 tgtctggttt tgtcctgcac ctcaatactg gcatttttgc tcgccacacc tgcaagaagc
    19681 agggtactga ttgcaaaaac aatcaacaat aagagaatac tttttacggg ctttctgaac
    19741 acagaacgcc ttgccaatcc aaaaaaattc atgtactatc cctccatttt cgctaatata
    19801 tcttttgggt ttgctcgtaa tatcggaaga atggagcaac acaatgctac caaaataact
    19861 gcaccgccta aaagaaacaa cattccaata tcttgtatct gcaacaccac tttcagattt
    19921 tctgttacgc tttctgaact ggtaatgatg ttttgcaaaa cgcccgagaa caatttacca
    19981 atggctgttg ctcctaaaac cgaaagcgta aatacactcc ctgcttctaa taaagtttgc
    20041 aggaaaattt caatttttgt cttaccgagg ctcataaaga ttgccacctc tttttgcctt
    20101 gtccgcatcc acatacataa cagcagggaa acaacgactg taccagttac taatgtaagt
    20161 actagcataa gatttgccac cttactgatt tgctctaatg gggcagacat ctgttgataa
    20221 agggtatcag acgtgctaag tgctacatca tttccgaata ttttttgcag gctggtttcc
    20281 agcacatcta atttttctgg tctttttgag taaatgcaga ttttttcaaa ttcagcttct
    20341 ttgaacaatt ctttataagt actgttgtca ataaagattt gattttctat tcgattgact
    20401 gcggtagttt ctgacggctg gtctttttcc gcatttcctg cggacatgaa aattccaata
    20461 atctgaacag atacttttgt tccgttttct gtacttagtt caatcgtatc tccaaccttt
    20521 agcccattct gatttgcaag gttcgtattg ataacagcac cttttttttc atgtttgata
    20581 taatcgccag aagctaagcg gtactgcatt tctgaaaagg gactgtcctt ttccaaatca
    20641 tcataggaaa gcagtgctat tttcaaattt tcctcattcg tagaagtatt gagcgtaacg
    20701 ggagcaaaat tattcggaaa tacctcttgt ctaccaatgc ggttgataga cgaaacatct
    20761 tctagcgttt caatcttgtt tacttcattc tctgttatct tgctgtcctt acttgtaatt
    20821 tcagccacaa tcttagagtt tgtcttttcc tgcatggctt gtttggactc atttgtagtc
    20881 cttaaaatca tgcttgtacc taaaatcata ctgtttacaa gtatcaaaac aatcaacaga
    20941 agaatggttt ttcctttttt tcgctgtaaa tagcgaaaac caagttgata aaacttcata
    21001 cttcacacct ctattcccaa cgagctacga caacctttgt tgcattgaaa tgatttgtgt
    21061 ctgcaaactc tccatagaga tatacttcag attgcttttt cacatcggat atagaacccg
    21121 ttgttacatt tgtaacacca gactgtttat ttagtgtcgc aacaacaaat tcacaatctg
    21181 ggttataggt tacagaaaca gcattgtctt tattttccaa tcccgctgct tccgatttta
    21241 ttccagctcc cccctcaatt tcttttgctt gacttacgga acaaccattg tctgtaaatt
    21301 ccaaaacact tcctatcaaa ttagcagaat ctaatatgtc gtttccttta ttttctgaag
    21361 aaccatttgc attttcttca ttcgtattag aattgacaat aggattagac gtatctggtt
    21421 tctcatctgg acttgcacca caccccgtca atgcacagat acataatcca actgctatga
    21481 atttactgat gtttgttttt ttcatttttg tattccacct ttcttgttcg atatgcccct
    21541 attgtaaaag caaaccattc taaaaatcct gtaaaaaagt aagaaaatca tcatcatttt
    21601 ttagaggatt tttacagact tttctgttat actgtctata attcaaactt cttggactgc
    21661 ttatgtccaa gatgatatat agaaaaggat tgatacatta tgaaaatact tcttctggaa
    21721 gatgatttag aattatgtaa aaatataaaa atttcgctgg aaaaagaaaa ctatatggtt
    21781 gattgttgca atgacgggga aactgctatg ctgtatgcct taaatacaga ctatgcttat
    21841 gaccttgcca ttgtagaccg tatgttaccg attattgacg gattaacaat cataaaatct
    21901 atgcgtagaa aaggaatatc tattccaatc ctcatcatta ctgccatgag tacccttgat
    21961 aatcgggttg agggtttaga cggcggtgct gatgattatt tagtaaaacc atttcatatc
    22021 aaagaattgc ttgcaagggt tcgggcattg acaaggcgac ctgccaatat ccagttatcg
    22081 cctaaattac aatatggaga tttaactttc gataaagaca gccgaacctt atctacaccc
    22141 gtcaattcta ttttattaac ttcaaaggaa accgaattga cttttgtatt tatgcagaac
    22201 ccagaaacgc tattttctcg tgaacagcta atccttaaaa tctggggcag ttcttccgag
    22261 gtagaaactg gcaatgtaga cagctacatt tcttttctgc gaaaacgcct gaaagagtta
    22321 aaaagttcct gtaaaataac aacggtctac ggtgcagggt acaaactgga gaaagaaaca
    22381 tgttaaataa tttataccga aaacttcata ttctgtttgc aagttctgtt atgctgatta
    22441 ttacccttgt gatttccttt gtcgttgcga acacagttca tacagagaaa atcaacgaaa
    22501 gcaccttgtt tcaaagattg acaaccttgc tgatttatca agtggaaagt gcttcttcgg
    22561 atatggacaa agcactcaaa acctacgaag aaagttatca tattttttct ttaattactg
    22621 atacaagggg gaataccatt tatcagagtg atttcccatt tccaacttct gcggataatc
    22681 tgttgcatga tgtagaaatg cagatttcta cacaaccatt atcacaaaca gaaagcaaca
    22741 caacttcaca aggcggtttt ttggaaataa gaggaacaca tcacgatact tattttgtta
    22801 ttcctgctac aattaggaca gcaaatgata ccgtgtatca tgcaaccttt ctttatcaga
    22861 cagccagttt gacggatatt ttacaaaaga cattgcctat atatcttctt atctggtttt
    22921 tggcttgtat cgttgttatc gtgctaaccc gttatctgct gaaaaagtcg tttgctccaa
    22981 cggaacgaat actgcaaagc caaaaagatt ttgttgcaac agcgtcccat gaattaaaat
    23041 ctccacttgc tgttatgatt tctaacacgg atatgcttct tgataatgtt tccttaaacg
    23101 agcaggcaaa gcaagccgta cagaccgtag attttgaatg tatgcggtta tcccgtttag
    23161 tcaaagatat gttgcttttg gcttcttccg acgctaaaac atggacattg cataaaagca
    23221 caatcaatgt agataccttg ctcattacgt tatatgaaac ctacgaaccc gtctgcatga
    23281 agcagaattt aaaactaaaa ctgcatttat ccgaggaaag ttatcctgcc atgcttaccg
    23341 acaaagaccg attatttcaa atcttgtgtg tctttatgga taatgccatt caacattcca
    23401 agaataattc tttgatagag atagaagtaa tcgccaccga aaaaaatatt gctttttctg
    23461 tggtagacca cgggcaaggc atttctgatg aagataagca gtatatcttt gaccgatttt
    23521 atagtggcga caagtcccat acgaacaaat cgaattttgg actgggacta agtattgcca
    23581 aagaattaac ccaaatgctg aacggtgcta tcactatcaa tgatactgct ggtggaggtg
    23641 ctacttttac tgttaaattt cctctgaaat agattaaggc ttgcagatca tacactatgt
    23701 atggagcaag ccttttttca aatctttgtc tttttggtaa tcttgtaaat atattcttct
    23761 ggggtagggc gtttattacc aaaatatttt ttgaaattcg cctgcacttc tggggcggtg
    23821 ctattcatag cttcatcaat cgtcgcttca atatctgccc gaacatcagc caatgataca
    23881 ccgccagctt ttgcaccagc tttcaacgct ttttttatat ctcgcttttt tagtcctatc
    23941 ataataacct ttcttatcat aattgcaaat ttataaattc attagtatta aaactgaaat
    24001 tcaaatacat tactaaaatc attgttcaag acataataaa ctttattttt ttctgtagaa
    24061 tatacaaatg aaaccctagt tgcccacaag tctttttgag aaacagattt taatacttta
    24121 aatgcgtcat ttactgaaaa atctttatca tatcctgcca ataactcttt agccacttcg
    24181 tatcttgtat ccccagaaac aatgtacggc tttgttatat ctggttttag aatagaataa
    24241 tttgtaataa gtgaaaaatt ttcttgttca tatctatacc caattccggg ttcaataatt
    24301 aaaactcgtc cttttttatc agaaagcata gcttgcatag tagcatctgg tgcataaata
    24361 atttttttct ttttaacaat gtccaaggct ttatcaaaag aaatctctgc cttaatgaat
    24421 ttttctgtta attcagatat tgtaatacaa tcctcgcttt ctatatacct tgcattttca
    24481 ttaccatgaa cataaagaag tgttcctaca ttaccatttt tatttatccc atgaaatgaa
    24541 tgatacatac cgtcaggcat tttaatacca atataaaatc tattttcttc ggtaattaca
    24601 gtatgatccc atacagaaag gtcaatatca aaattaaaac caacaatttt atctatacca
    24661 ttgtaaacaa atcttgtaca catataatca ctcctcaaaa tactacttca aatagttatt
    24721 taactaattc aattagtatt ttaaaatgtt tttgtaggaa agtcaataaa taatcataat
    24781 tttctaattg tgttattgac tacactaatt caattagcta gaattgtgtt tgtatgatat
    24841 tttgaatgag gtgcataact atgaaaacca ctaaagaagc agtaataaaa gcaacatcag
    24901 acttagcaga tgaaaaagga ttaaataacg tttcgttaaa agctgttgct caaaaattaa
    24961 acattcgtac accatcttta tataatcaca tagaaagttt agataatctt cttttagaag
    25021 ttgctcataa tggtatgcgt gacatgaatg aaagaatgat gaaaattgcc gttggaaaaa
    25081 tagggaaaga agcaattaag ttagtttcta tcgaatacct aaactatatg attgaacacc
    25141 ccggagttta tgaaacaatt caatgggctg tgtggcacgg aacagaggaa acagcaacca
    25201 tttttaacaa ctatctttca ttattaacta ctcttatcca atcgtgcagt cttaacaaag
    25261 acaaaacact tgaaatatta aatatgctta ctggaataat tcacggttat acaactttgc
    25321 aattaggaaa cgctttttct gcctcagata aagttagatt tgaattagct gaagcgatag
    25381 atactttatt agtcgggatt ttccagaagt ataaataata tatttaggct tgcaaatcaa
    25441 tacagaactg caagcctttt tcttttatcc aacaatcttc cagttgctat ccttatgtag
    25501 tgcaagttca tactgcgata cttgtgttgc ctttgtctga ttatcaagga attttacatc
    25561 aaccttaact ttcacattgt ccccgtcctt tgtaaagata gggtttacca gttcagaata
    25621 aaggtagtcc ctgccgatag gttcaatcac attccccgat acatagtagg caagctcttt
    25681 ttctgtggct gttggataga gcttaaagaa tgtttccaaa aatgcggtag cgtcattgac
    25741 agtatcagcg tctacgcttg cgtctgcttc tggtgtctta ggctcatagt ctgatttttc
    25801 gattgctggt gcaagggtag ggttctgaac gattaccata tctccgtcag cgtccacatg
    25861 gactttcacg gtataggttg ccttgacatt tcttgcctgt tctccctctt ttatctgctg
    25921 atctacttcg taggtagcag aaaaagtgtc cgttcccgac tgttcaatac tccatacaat
    25981 cacatctgta actgtggaac tggttggtat atccattctg attgtatcaa cattcaagtc
    26041 ctgcaattcc tttgtcagat aaccgttgat tgcctgcgtc cttgcttcaa tcgcttcttt
    26101 gctgttgctc catgtgtaat aggacttcgc aaagttcttc acgaaatttt ctatcccgtt
    26161 ggtgtcctgc aagcgaagct caatgatttc tttttcatgg gtcgtgtgct ggtcgatagc
    26221 ggtaaaattc ttatacactc caaagctcac gcttgcgata agcaccaccc acaacgcaat
    26281 cacggttttc ttatgtgtgc ctaccttgac agtacgcacc tttttttctt ttggttcttt
    26341 gatagtttct gtctgtttct tattcttctt aaacatattt tcaagtcctt tctattgttt
    26401 tactcgtcct gcaccgatta agtgctgttg ccagtaacta ctacttaggt ctgcatatcc
    26461 tatcgggtcg cctgcatggt acatctggtt atttcctaca taaataccga cgtgagttac
    26521 atacgaacca gcgttataag tggaatggaa aaataccaag tccccagcct ttgcctgcga
    26581 aagcggaaga tgttgggttg cgtcatattg tgcttgtgct gttctcggta aggagatacc
    26641 agcttttcca taacaccatt gcacaagtcc actacaatca aaggaagtgt tcgggttact
    26701 gccaccatat acatacttcc agccttgata tttcaacgct tcattcatta ccttttgtgc
    26761 cagttctcca gatacctgcg gaacagttaa atactgattg actaattccg catagaacat
    26821 atttccatag ccataccgcc agcccccgtt cttcgcaaca gctatcgggt tggtgtaggt
    26881 tactttcttt ccacccgact tctcacgggc gaagctctct gcaagattaa aagtgtgttt
    26941 ctttcctttt cctgccacat atcccacata gccaccgcca taattataag actgtatcgc
    27001 tacattcaaa tcgtcgatac cttgattttt gcaggaagaa agcagggacg caaaatactt
    27061 acacccctgc ttgattgagc tttccgtatc taaggaatta ggcggtaaac caagactttc
    27121 cgaactctgc ataacatctt ctgccgtacc gccactttct acttgaatga tagccaatag
    27181 cacattgaca tactcggaga taccgtattc tctggcgtat ttttctacca taggctgatg
    27241 tttcaagact tctgcggata agttcatacc tgtaatgcca gaagaaaaat tgctattctc
    27301 gtcgtcgctg tccgcactaa tcaacacccc aaagaaaagt accagcgaaa agaggatagg
    27361 aaacagactg ccgataagag cgatatgttt cagtttcatt ttttcttctg tcctttcttc
    27421 atcacaaggt tacgggtttt ctctgtggtc tgctggttct tctggacgct ctgtgtctga
    27481 tgaacctttg tcctgcggtc tttggtgtaa ttctggcgtg tttcctgcga taccactttt
    27541 tctacattct gcctatgtac tggctgtgca gactgggtcg ctttcctatc agaagcacca
    27601 gaagataacg gacgctcttt gataacattt gtcttgactg gctcacttgc ttttgaagtg
    27661 gtcgctgtgg cagggcgttt gacttcctgc gttttttcag cactcggctt tggaatgatt
    27721 gaagctgtgg ctggtcgctc atggggacgg gtagctcccg ttgtcgctga tccgtcagcc
    27781 tttcgctgta cctgccttgc ttcttgtgcc ttttgaagct ccatacgctt gtcagcgata
    27841 tttttcttat gctgttcctg cttttccaat cgtcccgtct gtctggattg ctgttcctgc
    27901 accatgccac gcttgaagtc ggacacgctg gactttgcct tttcctttgc ggaatacacc
    27961 gcataagcgg tctgtgtcgg catatccttg atattctctt tgacagcatt tgccttgtct
    28021 ttcactttat ttctcgtatc taagacagca cccacctttg agcctgcacg ctgtcccatg
    28081 ctggaagtgg tattgccccg actgtctttt gtagctgtgt tccttgtcgg ttttctgttt
    28141 gaaatcgtgt tacctgccac cgcaccagca acaccgccag ccgtaaccgc accagcaagc
    28201 cgtctttcca tacgcctagc cctatgtcgc ataaacagat acggtctgcg gaatatgcga
    28261 cgtcccatgc tttgactatc gccagcgttc aaagagaaca tattcattaa gtcgcctaac
    28321 ttgataaaga tacctgcaaa acacactatc tgtaagaacg ccaccataaa aaacgggtac
    28381 tctttggaga tgttataaaa catactggaa atactgaacg ccaccgttac aatgagcgta
    28441 attcctgccc gtgtcattat ggtattaaat accttcacga ttgcctgctt tgccagattt
    28501 tcttgactgg gtatcatgga aagaagaaaa ctgataggta aaaacatagc gaagataata
    28561 aaaagtatct ggcttaacat cattatccct gtcagtaaga atacaaagat tgtgatacct
    28621 atattaaaaa tgaataggaa gaacaccatg cctaaacggt tcacgacctg cggtatcgtc
    28681 agattgttgt tgtcattgtc ctcgatttct gttttcacga cttcttctct ggttttcccg
    28741 tcctcgtcct ctgggcttgc cgatacgaga gcttcgacac ggtctgttcc gattttctct
    28801 gcgttgctgt taccgaattg taagagtagc cacggctgtt ccacttgaat agaaaatagg
    28861 ctgtcccgta tcaagtccac gctgtccttg ccctcgctgt cagagttagg gagcatgatt
    28921 tttgttccca agtcaagtga agcggtacta atatcacttg aaaattcatt tatcttcttg
    28981 atgtagtcgg gagcataggc gataaacgaa gcggacagta cgaacaccac aagaaaattg
    29041 ataaccgctt gaaccgcttt gctggtttct ctttttatca gtcccgtata ggcaacataa
    29101 ataccaatga ttaaaattat cagtaataga gaacccacat aaaatcccgt actcccgaag
    29161 ccattctcgg taacacctgc aagggtctgt atgctttttc caatactgtc tgccatatcg
    29221 ttaataaaat caagtttata ggcttcctgc accacatagc ccgttgcatt gcttaaataa
    29281 aggcttatcg tccagacaaa gttggtaata cagtaaagcc cgtactgcac cgattttccg
    29341 ataccgtcca gccagttcca cggaagccac gaccagctat tatccacata aaaatcaagc
    29401 tggtagttgg aaaggggata ttttgaatat agattttctg cgtttatggt atcgtccaca
    29461 agccccgtcg catgagccac cgtccccaga agtgaaagca ggataaggga gagtgccacc
    29521 acgaacagag ccattttgag aaagtgaaaa atcttctttt ttgtgaacgc accttttatc
    29581 ctttctttca tcactccacc tcatttctct gtacgggcgg tctggtatca aaggcgtgta
    29641 gcagttcttc aaaaactggg tgtatctgca ctacaccgac acgcccgtat aagtcctgca
    29701 ataagcattg tccgttctcc aaatcacgca agcgtttctg gttgttttcg tcgtccttgt
    29761 cgataccgaa aaattctaag gtctgcttta tctcgcccaa gtcggtacta cgaaaggcaa
    29821 acttcaagcc gatattgttt ttcagacttt cctttgccac gtccccagaa gattgtgtaa
    29881 cgaaataaac gcctgcctgc atagctcgtc cagcacgaac cagcttgttt gataaggttt
    29941 cgccttgtgc cacatttaag aacgcccacg cttcgtcaag gtctacaatc ttaaaaatac
    30001 ttctatccga atggataaaa tcaagggcaa aggtactaat cacaatcagc atagacaccg
    30061 acaataattc aatggtcgtg tattcctcaa aagtggtatc tttatctggc aatactaagt
    30121 ccgctacttg aatgatattg agctggttat ccaaactgat agcattttct actgtaccgt
    30181 ccgaaaacag caggtgtgca aagtcgtagt ccgtgaagct gtcgatatgg tctgcgatat
    30241 ttcttgatat gagcgtatct tcacggcgta gctcgtctat cacatggagc aagccccgac
    30301 tgtcgctctg ggtaacggaa cgcaccgctt ttcgtaacac ggggaatttt tcgccgtccc
    30361 tagaggaaat acccgtaagg aatgttaaga tatcgattgc cagactttca gcgtctttta
    30421 cattcttcat aatcacgaat gggtcaagaa gacctgcatt gtctttatcg ctggttaagt
    30481 ttacgatatt gatttcatgg gctatctctg ggagcgtttc tttccagttg ccacgctctg
    30541 attttgggtc taagataacc gcttgtcccc caaagagaac agagtagtac acaagaagat
    30601 tgttgcagaa cgacttccca ccgccaagcg aaccaacaaa agcagaagca agagcatttg
    30661 taactgtgcc ttttacgccc tgcgaagcta aagacggttg caggtacaca tttcttcccg
    30721 tatcaacgga atagcccata tagatacccg tagtttctcc taactgctgg gtcgctccaa
    30781 agccaagccc agctaaaaaa tctgatttta catactgcac atagtcattg atatatcgct
    30841 tgccggcagg aagaaattca gaatgaagcc ccagcatatc cccagcagga cgcaccagct
    30901 ttacattgag gtcgtcgtaa aagtctttga cttcatcaca acggcgtttc agctcgtcaa
    30961 gatcgggtgc agacacccgt atcacgtagc ttaacttata catactttct ttgctctggt
    31021 ctaagtctgt ttccagctcg tccacgctgt ctaatgcgtc caccacattt gagctggttt
    31081 cacttcctgc ttgataagcg tgattgtcaa ggtctttcaa ttcctttttc ttgttgcgga
    31141 cggttgttaa tgcttttcga ttttccatga tttctacatt catagaagta tcaacgggga
    31201 atgtgaattg ctgttgctgg aaatagaaga tttcacttga cggaaaatca agctcgccga
    31261 caatcgcatt gacggtaaaa taggacacat agctttcctt gtcctcatgt tccaatcgta
    31321 aataccgctg gctttcctca atcacacacc ttgtcgggcg gataaggtcg tagtatttta
    31381 tcagcgtttc tttctgcaat ttcttctttg gtagctggta ctcataatct tcataggcaa
    31441 taccgtccct gccgtaaaga tgttccatga gatacccaaa atcattgatt tccaagcgac
    31501 gcaccttaaa gcgacgggag attttatttt ccagtaactt ttccattttc atgtaacggt
    31561 tgatttcatc attcggcatg gaaacaaaat cattcatcag cgtatggttc acttcatgga
    31621 gaaattccgt gaacgtcagc cacgccgatt ttttgatgtt cttcagattg agctgttctt
    31681 ccgtaaccat gagcttaaag ccaagaaaaa agcggtagtc cacttgattg tccccaatca
    31741 tagatactaa cgcttcggtc tgttcgtcta tcttctgata ggcaacttcc tttaattttc
    31801 cagttaccag cttctttgac tgttcctgct gacttcggat tgagctttct gtggcaatct
    31861 gcaacgcatg aattttaccc tcacgggact gtacgattag ctgtcggaaa ctgtcatgca
    31921 cgataaattt ctgctctgcg gatagaaaag aatagttata cggtatcagc tcatagtaag
    31981 cgaacacctc attgtccttg ttccagacaa ggttattgtc aatatatttt atcgggaaca
    32041 tagttcacac tccttactgc cgtgattgtt tcattcaagg tctgcttatg cagttttacg
    32101 gcttttcctg cataagtgat tttaggtcgc agagcatagg ttatctgtga tttcaaaaag
    32161 ctgtacggct tctttccgtc aaaggttttc tgcgacataa accaagtgag agcaacggga
    32221 ataccgaagt atttgagaaa tgctccctca atcatggaaa gtgggggaat atccccaaac
    32281 agaatgatga taaattccgt aatcacaaac catgtaatct gtgtaaaagt aacgggaaag
    32341 ggtaagttaa agtcattgat tgcatacaag actttttcca cgttccagat acccgtataa
    32401 gatttaatct ttttcaagtt ctttcagctc ctttcaaaat agaaaaggac agccatgttt
    32461 cagactgtcc cttaaaagag aaatacgctg tcagtagcaa caatgcttgt cgctgatctt
    32521 ccagcgttaa tatggtatct cgcacatacc tcggcttgtt tcaataaagc aaccgcccat
    32581 atccaaatct cgcccgtagg cttcatagtc aatgtaattc tgtaagctgg gtggaatatc
    32641 tcctagtgcc tgcaattcgt ctatgtagta gtaggcaacg tctgtcatgg tttcacagtc
    32701 gggataataa taaatatcgt ccttgtgttc cacgacttct tccagcgtcc catagtagct
    32761 gataaattcg tccagacatt ctacgatata gtcgggaagt tcctctatca tttcatacat
    32821 ctcgttgagt tcttcaatgg aaacatactc gccaatcgca atggggaagt tgtcggtatc
    32881 atggatagcg tattcctcat actgttcatt caagccgatt ttttctttca catcttcctc
    32941 gtcaatgggg aacgtgaacc aagcaccgac taaatagccc tcattgtatt tgccaagatt
    33001 agcaatgtaa accgccatat catcaatcat aagcaccacc tcatttccta ctctggcagt
    33061 tcaaagatac cgtgttctgt tactaaaaat ttcccgtgtt cctgcaagtg agaagcgtag
    33121 gcttcaaagt caaaatagta ttcttgacag tcctcggata aatgcttaaa tgtcgggtcg
    33181 ttcatcagct tttgctttgc aacatctatc atgcttttgc agtcgggata aaccgtaatc
    33241 acgttcctgc aaatataaag tgcttctaaa ttttcatata cggtaagaag tgatacatat
    33301 tcttcctgca tatcacttga aagctggcgg tacataaaat ctaattcgtt gagctgatac
    33361 acgcttgtat gttcgtggac ttcatcagca aagggcagta ccttttcgat aatgcggtaa
    33421 tccccacttt ctgcaccgac acctaacttt tcttcaaact cggcaacatc tatcggcagg
    33481 tcaaaccagt atgatttcgt ttcttcgcca gttgttcctc tcgtttccac cagcaccctt
    33541 gtttcctgca ttgttcctca cgtcctttct gttgtttcat cacatctttt aaggtctggt
    33601 acgacatacc aaacacatac tttgaaaaat cttctaactg tctttcttca tagtcagcag
    33661 ggttcagccg tttcatttca tgccacacct tacgcttgta agagggcagg tcggaaatgt
    33721 attcacagcc gacgtttgcc tgcatatcct taaaaatatc gttcatttca tcactccaat
    33781 ctgaaattta ggtatgaaaa aagcaggaga ccttttcaga tttcctgctc agtagagttg
    33841 tgaagatgtt attgttgtta tttaatttca atgttcgtca tagttactaa cccaagacga
    33901 actaaaatag tttgtacgat acgataccaa aaaacaccta gaattaggag taaagcatat
    33961 aaaataattg acgcttgtaa atattgtctg ccaaagtgaa taagaataag cagtggagac
    34021 attaatatta caaagaatat aatgcttgta attatttttt ttatgttttt tctatctgct
    34081 aaatcaaatt cagctccaca agaacatttg ttaggtattc ctccttttat ttgagcacca
    34141 cagttcatgc aatatagttt cttcattcta atcacctcta caacctctaa ttcgtcgttt
    34201 tcttcatatc cacattataa cattcgcata tcaacactac aataaaattt tatgctccga
    34261 taatcttatt gaacagctct aacagtacat tatttcatca ctccaatctg aaattttggt
    34321 aagaaaaaag cagtcaatcc taagactaac tgctttgtgt gtagggatat gaaattgtaa
    34381 aactggaatt ttttctttga aagctcttaa acaaatgaag cataaataac ataaatttct
    34441 gcaatcaaaa caattccaag aactccatct atcatcaaat tatactgtat atctgtcaaa
    34501 aaagtttttg aatgtgttaa gaaatacttt attttttctt ttccagttaa aatcgtaatt
    34561 ataagtaata aaataggaaa caaaaaaatc tctatcttat ttccaaaatc atctgctatt
    34621 ccatttgcaa aatgcacagg aataatttga ggtaagaaaa gaacgcctac taacgctaat
    34681 aaaataccaa taccgcaaat tccccatgtt aattttctgc ttcctacaag tttcataaaa
    34741 atgccttctt tctgctaaat tataatgttt tgcttttcat atccacatta tagcactcac
    34801 atatcatcac cacaataaaa ttttatgctc cgataatctt attgaacagc tctaacagta
    34861 catctttcac accgccagca ttgaacacca aaccgacagc aattaaggca actaccaaga
    34921 agccgataag tttggaaaac tcacgcttga accctaagta aataccgata accacaatcg
    34981 ccataagcac taagctctgt gc
//
LOCUS       DAEMIK010000023        34933 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEMIK010000023 DAEMIK010000000
VERSION     DAEMIK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.023"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            16..156
                     /locus_tag="KPK87_000978"
     CDS             16..156
                     /locus_tag="KPK87_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537151.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(331..651)
                     /locus_tag="KPK87_000979"
     CDS             complement(331..651)
                     /locus_tag="KPK87_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG2537152.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(771..1508)
                     /locus_tag="KPK87_000980"
     CDS             complement(771..1508)
                     /locus_tag="KPK87_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG2537153.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1723..3546
                     /gene="acd"
                     /locus_tag="KPK87_000981"
     CDS             1723..3546
                     /gene="acd"
                     /locus_tag="KPK87_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG2537154.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESNGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3761..8059
                     /locus_tag="KPK87_000982"
     CDS             3761..8059
                     /locus_tag="KPK87_000982"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG2537155.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8263..8724
                     /locus_tag="KPK87_000983"
     CDS             8263..8724
                     /locus_tag="KPK87_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG2537156.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8754..9908
                     /gene="nusA"
                     /locus_tag="KPK87_000984"
     CDS             8754..9908
                     /gene="nusA"
                     /locus_tag="KPK87_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG2537157.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            9915..10196
                     /locus_tag="KPK87_000985"
     CDS             9915..10196
                     /locus_tag="KPK87_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG2537158.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10186..10497
                     /locus_tag="KPK87_000986"
     CDS             10186..10497
                     /locus_tag="KPK87_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG2537159.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10519..12459
                     /gene="infB"
                     /locus_tag="KPK87_000987"
     CDS             10519..12459
                     /gene="infB"
                     /locus_tag="KPK87_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG2537160.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12502..12882
                     /gene="rbfA"
                     /locus_tag="KPK87_000988"
     CDS             12502..12882
                     /gene="rbfA"
                     /locus_tag="KPK87_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG2537161.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12863..13540
                     /locus_tag="KPK87_000989"
                     /pseudo
     CDS             12863..13540
                     /locus_tag="KPK87_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
     gene            complement(13774..13923)
                     /locus_tag="KPK87_000990"
     CDS             complement(13774..13923)
                     /locus_tag="KPK87_000990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537162.1"
                     /translation="MQAQTISYPSALSVKVYPLPLPIACSVLPSRGIVVEVSPIRSLP
                     ADCPF"
     gene            14187..15305
                     /locus_tag="KPK87_000991"
     CDS             14187..15305
                     /locus_tag="KPK87_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2537163.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KSDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKIRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEVFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            15423..15728
                     /locus_tag="KPK87_000992"
                     /pseudo
     CDS             15423..15728
                     /locus_tag="KPK87_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            15741..16646
                     /gene="truB"
                     /locus_tag="KPK87_000993"
     CDS             15741..16646
                     /gene="truB"
                     /locus_tag="KPK87_000993"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG2537164.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCDVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            16777..17706
                     /locus_tag="KPK87_000994"
     CDS             16777..17706
                     /locus_tag="KPK87_000994"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG2537165.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGYDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            17810..18067
                     /gene="rpsO"
                     /locus_tag="KPK87_000995"
     CDS             17810..18067
                     /gene="rpsO"
                     /locus_tag="KPK87_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG2537166.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            18307..19152
                     /locus_tag="KPK87_000996"
     CDS             18307..19152
                     /locus_tag="KPK87_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2537167.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELFEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            19277..21388
                     /gene="pnp"
                     /locus_tag="KPK87_000997"
     CDS             19277..21388
                     /gene="pnp"
                     /locus_tag="KPK87_000997"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG2537168.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            21654..22397
                     /locus_tag="KPK87_000998"
     CDS             21654..22397
                     /locus_tag="KPK87_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2537169.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            22555..23802
                     /locus_tag="KPK87_000999"
     CDS             22555..23802
                     /locus_tag="KPK87_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2537170.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTKNLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            23837..24082
                     /locus_tag="KPK87_001000"
     CDS             23837..24082
                     /locus_tag="KPK87_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG2537171.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            24092..25315
                     /gene="dapG"
                     /locus_tag="KPK87_001001"
     CDS             24092..25315
                     /gene="dapG"
                     /locus_tag="KPK87_001001"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG2537172.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIESVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            25428..26162
                     /locus_tag="KPK87_001002"
     CDS             25428..26162
                     /locus_tag="KPK87_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG2537173.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            26285..28696
                     /locus_tag="KPK87_001003"
     CDS             26285..28696
                     /locus_tag="KPK87_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG2537174.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDDEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKAEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVIE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            28699..29757
                     /gene="mnmH"
                     /locus_tag="KPK87_001004"
     CDS             28699..29757
                     /gene="mnmH"
                     /locus_tag="KPK87_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG2537175.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            29751..31085
                     /gene="rimO"
                     /locus_tag="KPK87_001005"
     CDS             29751..31085
                     /gene="rimO"
                     /locus_tag="KPK87_001005"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG2537176.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            31072..31614
                     /gene="pgsA"
                     /locus_tag="KPK87_001006"
     CDS             31072..31614
                     /gene="pgsA"
                     /locus_tag="KPK87_001006"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG2537177.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFVIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            31902..32948
                     /gene="recA"
                     /locus_tag="KPK87_001007"
     CDS             31902..32948
                     /gene="recA"
                     /locus_tag="KPK87_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG2537178.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            33124..34665
                     /gene="rny"
                     /locus_tag="KPK87_001008"
     CDS             33124..34665
                     /gene="rny"
                     /locus_tag="KPK87_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG2537179.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        14177 a         3728 c         6652 g        10376 t
ORIGIN      
        1 gagagggtgt aacaaatggg aataatagag tcagcatcaa aattggctga gatggttcat
       61 ttattggctg ttgaaaaggg gataactgat atagaagcat gggatgaagc tgtgaaagaa
      121 tacagcaaaa tttatgaaga aagaagaaat gaatagaatg ataaaaaatg aaaacttgat
      181 gataaaaaat aactttattt aaagaaattc aatttttata gaagtattaa aaataatata
      241 aataaaaaga tggtatttca aatgtaagta aaaaatcatt ttgggatacc atccttatta
      301 tttattttaa taatatataa gtaataaatc ttacttatcc atatttttta tgtaacctct
      361 tttttctaac ttttttatat gctccttacg tatttcatca agattaatac catattgttc
      421 ttctagctta aacatcatag taacacatgt ctgcgctaca tccattagtt ccttagcagt
      481 tttctttata atatcaactt catctaaatc attattttct ccacttagat ttctgaattt
      541 acctatacat tcagctaatt caccttgttc ttcacccatt ttgataaatg tagattctag
      601 tgttgggtct aaattattaa gctctggtaa agatatagtt tttagtttca taaaagctcc
      661 tttcaagtta ttcgtattta tactaaaaca tcataacaaa aataatacta aaaaaaaact
      721 ttaaattcaa cataaaaact tacatatata ataaaaatcg tgaaataaat ctatatttgt
      781 cttaatacag cctcatataa aacttctcca ataggtttat ttattacaag agaagctgac
      841 ttatcataga ctgtatttgc tttattaata agagcaatat aatcaccttt gtaataatct
      901 ataaaacttg ctgctgggta cacagctaat gatgtacctc ctataatcaa taaatcagca
      961 ttacttatag cagaaattgt tttagtaatg acatcactgt caagagcttc ctcataaagt
     1021 actacatctg gttttactat agaaccacag ttgtcacagt aaggaatatt acctcccaga
     1081 tttaacatag attctaagtc aaaaaactta ccacatttag tacaataatt tctatgtaca
     1141 gaaccatgaa gttctagcac atttttactt ccagccattt gatgaagtcc atcaatattt
     1201 tgagttatga cagctttaag cttacccatt tcttcgagtt ttgctaaagc tatatgagca
     1261 ttattgggtt ttgcattagg atatattaat ttatccttat aaaattcaaa aaattcttct
     1321 ggatatctaa caaaaaaagt atgtgaaact aattgttcag cagtaaattg tttattgagt
     1381 ttttggctaa ataatccagt agaacttcta aagtctggaa tattagattc agttgaaaca
     1441 cctgctccac caaaaaatac aatattattg tgattagcta ttaagtcttt taagctattt
     1501 gcatccatag ttacacctcc aaattattat atataaattg tactacatat taggtcgttt
     1561 atgttttatt aatttaaata aatatatagt ttaataagtt aaataaaggg aatataaata
     1621 aaatgtatac agagtataaa tagacaatat ttactaaaat aacaagttgt cgtataatta
     1681 tattaaaatt gttttagtgt ataagaaggg cgggggaagt acgtgaagaa aaaagcagct
     1741 ttagcaacat tggctatgtt acccttaggt gtggtaaatg cacatgctga tggagatata
     1801 ggtatagtga ctataaatta tttaaatgta agaaatgaac caactgccga aagtagcata
     1861 gcctttgttg ctaaaaaaga tgataaagtt ttgattaaag actcttctaa tggatggtat
     1921 aaaataaaag ctgaatctgg acaagaaggt tgggcttcat caaaatatat agcaaaatca
     1981 aatagtgact ctttaagaac gtctacaaat aaagaaaaac aagttatttc aaatagttta
     2041 aatatgagaa atggagcagg tacaagttac agagtaataa ctgtactaaa aaaaggtcaa
     2101 aaagtagaag taatatcaga gagtaatgga tggtctaaaa tcaaatatga tggaagacta
     2161 ggatatgtat ctagctctta tttaggagac gtttcaaact caactaataa atcaaaaact
     2221 aaacaagtga atacaacttc actaaatgta aggagtggac caaatacaag ttatggtttg
     2281 ttaggcaagt tgccaaaagg aagtaaagta gaagtaatat cagaaagtaa tggatggtca
     2341 aaaataaagt ataatggaaa agatgcctat gtatctagta tgtacttatc ggatgtaagt
     2401 caaagtaatt cagacaattc tagtcaaagc aatgataaaa agaatactga taaggttgta
     2461 aatacagctt ctttaaatgt aagaagtgga ccaggttcta catatagtaa gttgggaaaa
     2521 gtttataaag gaagtaaagt aactgtacta tcagaaagta atggatgggc taagattaat
     2581 tttaacaata aagaagcatt tgtagtaggc aattatttat ctacttcagc agatacttca
     2641 aataataact caaatagtaa ctctgataac agttctaata gtaatggcaa taattcttca
     2701 tcatcaggtc aagtaaatgg tatgtcaggt ataagtgggg ctaaaattga ttataaatct
     2761 ttgagttata ctttagaatc tcatataagt aaacaagttg agaaagcagc atcaggagga
     2821 aatgtgatag ctccaagtaa taggaaaagc actccaagtc ctgaatttag tactttttca
     2881 gctcaaagaa caagctcatt tgtaaatgca agttcaagtg atatagaata ttatttaaat
     2941 cctaagaatt ttacaaatac tactaaaggt atgatgcagt ttttaaagat taacagttat
     3001 agagatggta tttcagaatc aagtcttaac tcgtatctta atggtttatc ttctagtgta
     3061 tttaaaaacc agggagcagc ctttataaat gctgccaaga agtataatat agatgttgta
     3121 tatctagtat ctcatgctat gtgggaaact gcttatggta agtctacact tgcccaaggt
     3181 caaacattaa catcttataa gggacaacct cttagtaagc cagttaaagt atacaatttt
     3241 tttggtatag gagcaataga taaaagtgct aatgtttcag gtgcagaagc agcgtattcc
     3301 aatggctgga ctagtgtaga agctacaata gatggctctg caaagtggat atcacaaaat
     3361 tacgtaaata gttctaagta caatcagaat actatttaca agatgaagtg gaattatgat
     3421 tatacatggc atcagtatgc aactgatgta aactgggcta atggaatttc tggaattatg
     3481 gaaaatttaa ttggtcttta tggaggagga agtagtttgg tttttgaagt tcctcaatat
     3541 aagtaataca taaataaaaa atatagacta tgggtagttt tcattttatt taaattatct
     3601 atagtctttt tgtatataat ttataatctg taaatttaat gttaacaaaa cttttatgtt
     3661 ataatattaa ttctaagtaa atataagtga gtaatgggta gaaatagata aaaagtggta
     3721 ataatatata taaagataat tttgagaaag gaggatatat atggaaagta ttaaggaata
     3781 tttggataag ttagaaatta ataatagtgg tcttggaaag caacttaaag aagtttatat
     3841 aaatagagta gtttatttta aggaagataa gatagtttat ttttatctaa cttctaaaga
     3901 tattgtttct catgaacttt tagataaatt taaagaagaa ttgatgtata aactcgatta
     3961 ttttaaagat atgaggatga agataagatt tactggtctt gaaagaaaat ccaataaaga
     4021 tgttataaaa aaatattgga ataatatatt gtatatatta aagtatcttt gtccatctat
     4081 tgcagggtgg tataaacaag tagaatttct ttgtttagaa gaagaattaa aaataaaact
     4141 tccaaaaggt atattttatg agcgattaat gaaaaaaaat gtaacttatg tacttaaaac
     4201 cgtgcttagc gaagagttgg gattagattt aaatataaca atagaaaaag ctgttgatga
     4261 aaaggtaaat aaagaaagac tcataagaat caatgataga gaaatggaag aaaaaattag
     4321 ggctttagaa attggaaaag ttaataactg tgaagaaaat gaagaggaaa gttatgtcat
     4381 taagtctgat gtagatgaaa atttaatcta tggagataat gcaaatgcta tgattgaaaa
     4441 tatagttgaa ttaaatgcat cttctggaac agttgcagtt gttggagata tatttgatgt
     4501 tgacactaaa gaacttaaaa acggaaaaat acttatgata gcttcaatta cagattatac
     4561 tagctcaata agctgtaaac tttttttaac agatacaaat aaggatggag tacttgaaaa
     4621 tgttaaaaaa ggagcatact taaaaatcaa gggtgacata gtttatgata catatcaaag
     4681 agaaattagt atgatgatta gtgggataag aaaagaaact agaatagaga gagaagataa
     4741 atcagaggaa aaaagagtag aactccatgc acatacacaa atgtcttcta tggatgctat
     4801 ttgttctgtt aaaaagcttg tagagcgtgc agctaaatgg ggtcattctg ctattgctat
     4861 aacagaccat ggtgttgtac aaggatttcc agatgctatg aatgcaggta agtcaaataa
     4921 tataaaaata ctttatggag tagaagggta cttggttgaa gatgattctc tcattataga
     4981 ggatgcaaat gataaagaac tttcacagac atttgttgta tttgacatag agacaactgg
     5041 tttttctaat acaaatgata aaataactga gattggtgct gtaaaaatag aaaactttaa
     5101 gattgtagat agatttagtg agttaataaa tcctgaaaaa gatatttctt ataagataca
     5161 ggaattgaca ggaataacta atgaattaat taaagacaag ccaaccattg aagaagttct
     5221 tcctaagttt atggaatttg ttggagatag tgtattagta gcacataatg cagagtttga
     5281 tactggattt atatcgcaaa aatgtaggga acaaggcttg ttatataata acaaaaaagt
     5341 agatacactt atgttagcaa gagtaatgtt gccaaactta aaaagacata gattaaatgt
     5401 cgttgcaaaa gaacttggta ttcctctttt aaatcaccat agagcagtgg atgatgctga
     5461 agctacagca cttatattta ataagttttt gcagatgctt acagaaaaag gtgcaaaaac
     5521 tctaagtgat gtaaacaata tacttggaaa gattgattac acaaagctta gcacaaatca
     5581 tataaccttg atagcaaaaa attctatagg tataaaaaat ttatataaaa taatttcaga
     5641 tgcacatgta aatcattttt ttagagcacc aagaatcctt agaagtgtat taaatgaata
     5701 taaagaagga ttaataattg gttcggcttg tgaagctggg gtggtttttc aagctgtaaa
     5761 gaaaaacgtg tctgatgagg aaatgaaaaa aataatagat ttatacgatt atattgaagt
     5821 aatgcctata gataataata gatttatgat tgataaagga gaagtaaagg acgaagaaga
     5881 gttaagggag ttgaatagga aactaattga tacagccaaa aaatttgaca aaattccagt
     5941 agctactggg gatgttcatt ttttagacaa acatgaagct gtcttaagaa aagttcttaa
     6001 atattctcaa ggttttaaag tagatgaaga agaaacttat ttacatttta gaacaacaga
     6061 tgaaatgttg gaagaattta gttatcttgg agaagaattg gcatatgaag ttgtagttga
     6121 aaatagtaat ctgattgccg atatggttga agatataaaa cctataccag atgatactta
     6181 tcctccaatt atagagggct cagatgtgga gttaagagaa atgtgttaca aaaaagccaa
     6241 acgaatctat ggagacccaa taccagaaat agttcaaaaa agactggata gagaattaaa
     6301 ttcaataata agcaatgggt atgcagttat gtatattata gctcaaaaac tggttgcaaa
     6361 atctctttca gatggatatt tagttggttc aagaggctca gttggctcat cttttgcagc
     6421 tactatgagt gatataacag aagttaatcc tcttccagca cattatattt gtgaaaacga
     6481 agactgtaaa tattcattct tttatgaaat aggtgaatgg ggctcaggtg ttgatttacc
     6541 agacaaggat tgtccaaagt gtggaagaaa acttgctaaa aacggtcatg atattccatt
     6601 tgaggttttc ttaggttttg aaggtgataa agaaccagat atagacttaa atttctcagg
     6661 aaattatcag ccaacaattc ataaatacac agaagaacta tttggagagg ggtatgtata
     6721 tagggctggg actataggta ctgtagcaga aaaaacagct tttggatacg caagaaaata
     6781 tgtggaagaa aataatataa gtgtaccaaa tgcagaagta cttaggcttt caaatggatg
     6841 tactggagta aaaagaacct caggacaaca tcctggtgga gttatggtta taccagacta
     6901 taaagatgta tacgatttta cacctataca gtatccagca aatgatactt cttgtggtgt
     6961 tataactact cactttgatt atcattcaat aagtggtaga atacttaaac ttgatatact
     7021 tggacacgac ggtcctacca ttataaggat gcttgaagat atgactggaa taaatataac
     7081 agaaattcct ttagatgata aagagactat gtctctattt acatctacag aagcattagg
     7141 tgttacacca gaagaaataa actgtcctat aggttgcctt gctgtaccag agtttggaac
     7201 taagtttgta cgacaaatgt tacttgacac aaaacctact acatttgcag aacttgtacg
     7261 tatatctggt ctctctcatg ggacagatgt ttgggtaaat aatgcacaag acttagttag
     7321 agaagatata gttggattaa aagatgttat atctacacga gatgacataa tgaactattt
     7381 aatatttaaa ggattacctc ccaaaatgag ttttacaata atggagagtg taagaaaagg
     7441 taaaggttta aaaccagaac acattgaaga aatgaaaaaa aataatgttc cagaatggta
     7501 tataggctca tgtaaaaaga ttaagtacat gttcccaaaa gcacatgctg ttgcttatgt
     7561 aatgacatca tttagaattg catattgtaa agttcactat ccagaagcat tttatgcgac
     7621 ttattttacg acaaaagttg aggattttga tgcagattta attgttaaag gattagatgc
     7681 tataaaatca aaaataaatg aaattgagtc actgggaaat gatgcaacca caaaagaaaa
     7741 aggtatgttg accgttttag aagttgctct tgaaatgtat gctagaggaa taaaattatt
     7801 accagttgat atatataaat cagatgcaac tgagtttata gttgtaggag aaaaaacatt
     7861 acttccacca atggcagcaa tacaaggtct tggaggaaat gcggctataa acatccaaaa
     7921 tgaaagaaaa aatggtgaat ttatatcaaa agaagatttg agaaagagaa caaaaatatc
     7981 aaaaacggta atagaaactc ttactaatca tggttcactt gagaatatga gtgagagaaa
     8041 tcaattgtca ttgttttaag ttagcccaaa aagttgcaaa taaaagaatt ttatgatatc
     8101 atagtataga aaatagtata ctttgcagaa actaagagtg agacagctgt tctcactctt
     8161 ttattttaaa tatattatta aaaatctggt ttatttttat taaatatatt ttatttaaga
     8221 ttatataaac tggtcattta caattgaata ggaggaactt taatgaaaaa aaatttagaa
     8281 gctacaattg aggagattgt aactaaaata acggatgaac atggatttga aatggttgat
     8341 gttgaatatg taaaagaagc aggagaatac tacttaagag tatacataga taaagaagaa
     8401 ggaatatctt taaatgaatg tgaattagtt agtagagaat taagtccaat actagatgaa
     8461 aaagacccta taaaagaaaa ttacttttta gaggtatctt ctccaggact tgatagagca
     8521 ttaaaaaaag acagagattt tgttagatat caaggtagag atgtagattt aaaattatat
     8581 aaaccattaa atggatgtaa gcaatttgaa ggtgaacttg taggattaac agaagataat
     8641 aatataaaaa tcattgcaaa tggtaaggaa atggaattta atagaaaaga tgttgctatt
     8701 gtcagattag caataaagtt ttagtggtat attaaatagg gaggatataa aaaatgaatc
     8761 atgaatttat ggaagcatta gatgaattag tagttgacag aggtatagat aaagaaatac
     8821 ttatagatac tatagaacaa gctcttttaa ctgcatacaa gaaaaacttt ggttcagcac
     8881 aaaatgttag agttgaattt gatagagaca agggagatat aaaagtattt tctcaaagag
     8941 ttgttgttga tgaatcagat ttatatgata ctttcttaga aatagagtta gcagatgcta
     9001 gagagataaa tcctaattat gaattaggtg atattataga acatgaagtt acacctaaaa
     9061 actttggaag aattgcagct caaacagcaa agcaagtagt tgttcaaaga ataagggaag
     9121 ctgagagaga aatagtgtat aatgaattta tagaaaaaga aaatgaaata gttacaggag
     9181 aggttgctag agcaaataaa aatgttgttt atgtaaatct tggaagaata gaaggtataa
     9241 tgactcaaag tgaacaaata ccatttgaga attataaagc aggtcaaaaa ataaaattct
     9301 atatacttga agttaaaaag actaacaaag gacctcaaat agttgtatca agaagtcatc
     9361 cgggtctagt aaagagattg tttgaagaag aagttcctga aatatatgaa ggaatagttc
     9421 aaataaaatc tgtagcaaga gaagcagggt caagaactaa aatggcagta agatctatag
     9481 atgaaaaaat agacccaatt ggagcttgtg ttggaccaaa aggacttaga gtaaaaaata
     9541 tagtggatga acttggcgat gagaaaatag atataataaa atatagtgaa gacccagcag
     9601 agtttatatc agcagcactt agcccatcaa aagttgttag agtagatata gatgaagatg
     9661 aaaagtctgc cttagtaatc gttccagatt atcagttatc gcttgctata ggtaaagaag
     9721 gtcaaaatgc aagactagca gcaaaattga ctaactggaa aatagatatt aagagcgaat
     9781 ctcaagcaga acaagaaatg ttaaatcttg aaaattccaa agaaatagaa gctgaggatg
     9841 ttgaagctga atttatcgac caagaagagt tagatacttc agaggataat tttagtgaag
     9901 aagagtaagg agggatgatt ttgaaaaaag taaaaaaagt gcctcaaaga aaatgcatag
     9961 catgtcaaga tagagactct aaaaaagagc ttataagaat agttaaaaat aaagaaggaa
    10021 aaatattttt agacccaaca ggaagagcca atggaagagg agcatatgta tgtaaaagct
    10081 ctgaatgtct aaaaaaagct ataaagagta aagcattaaa taaagctttt aaaatagatg
    10141 ttcctgatga agtctatgat aatctactta tggagttaga agaatatgaa aaataataaa
    10201 gaaaaagtac tttcgttctt agggctcgca actcgtgcag ggaaaatagt gtctggtgat
    10261 gattcaactt tgcttgacct taaaaaagga aaagtaaatt tgattttaat cgcagaagat
    10321 gcatcaaata atactaaaaa actttttaaa gataaatcaa cttttagaaa tattccatat
    10381 ctattttttt caacaaaaga ggaaatagga tttgcaatag gaaaatctcc aagagcagtt
    10441 gtaggaataa aagatgaaaa tttttctaaa aagataattg aactaataga aatttaaaat
    10501 aaaatagggg gtgatcttgt gtcaaaaaca agagtatacc aaatagcaga agaattaaat
    10561 atatcaaatg aagagcttat aaataagtta gctgaattag atataaatgt aactgacaaa
    10621 gattcagtat tagaaggtga agagttagaa ttagcacttg aaatgttagg tgaagattta
    10681 tctcaagaaa atggaaatgt tatagaaata gatggaaagt tgacagtaca agtattagca
    10741 acgaaattag ataaatctcc atcagaaata attatgaagc ttatgaaaat gggaactatg
    10801 gcaacaataa atcaagagat aagttttgag attgctgctt tggctgccaa agactatgga
    10861 tttgaattaa cagtagctga aagtgatgat acagaagctt tagaaataga agctttaatg
    10921 gaaattgaag aagacaaaga agaggattta aaacctagac caccagttgt tacagttatg
    10981 ggacatgttg accatggtaa gacatcttta ctagatgcaa taagaaaaac tgatgtgatt
    11041 tcaggtgaag ctggaggaat aactcagcat ataggtgctt cagaagttaa aatcaatgga
    11101 cacaaaatag tatttttaga tactccagga catgaggctt tcacatcaat gagagcaaga
    11161 ggagcacaag ttacagatat agcaatatta gtagttgcag cagatgatgg tataatgcct
    11221 caaacagttg aagctataaa ccatgctaaa gcagcaggtg tacctttaat agttgctatt
    11281 aataaaatag ataaaccagg tgcaaaccca gataaggtta aacaggaatt agcagaccaa
    11341 ggattattag ttgaagactg gggtggagaa gtaatagcag ttccagtatc agctaagaaa
    11401 aaagaaggaa tagatacact tttagaaatg gtattattgg ttgctgaaat ggaagagtta
    11461 agagctaatc caaataaaag agcagtagga actgttatag aagctgagct tgataaagga
    11521 agagggccag ttgcaacagt acttgttcaa ggtggtactt taacagtagg tgacccaatc
    11581 gttgcaggtg ttgcatgtgg taaagttaga gcaatgataa atgcaaaagg taagagagta
    11641 aaaacagcag gaccatctac agcagtcgag atattaggtc tatcagaagt tccacaagga
    11701 ggagaccaat ttgtcgaagt tccaactgat aagattgcta gaagtgtagc agcaagaaga
    11761 caacaaatag ttagagatga aatgttaaaa tcaactcaaa gattatcact agatgctcta
    11821 tttagtcaaa tgagtgaagg ttctataaaa gaccttaata tagttataaa agcagatgta
    11881 caaggttcag tacaggcagt taaacaatct ttagaaaaat tatcaaatga agaagttcaa
    11941 gttaaagtaa tacatggtgg tgttggagct gtaacagaat cagacatatt acttgcagca
    12001 gcttctaacg caataataat aggattcaat gtaagaccag ttccaggtgc agaatctcta
    12061 ggagaaaaag aaaatgttga tataagaaca tatactataa tatacaaggc aatagaagat
    12121 atacaagcgg ctatgactgg tatgttagac cctgaatatg ttgatgaaga aacaggaaaa
    12181 gctgaaataa gagaaatata taaaatatct ggtgttggaa cagtagctgg atgttatgtg
    12241 actaatggta agatatttag aaattgtaaa gtaagattag ttagagatag cataataata
    12301 catgaaggtg agcttgcagc acttaagaga tttaaagatg atgtaaaaga agttaattct
    12361 ggatacgaat gtggtatgag ttttgtaaat tataacgaca taaaagaagg cgatatagta
    12421 gaagcttata taactaaaga agtagaaaga aaattataaa ttcgtatcaa agaaaatcag
    12481 ttagtaaaga aggtcgatgg tatggcatca tataatagaa caagaaggat agcagaagaa
    12541 ataagaaaag tcgtaagtac tatgcttata aatggagtaa aagaccctag aataacatct
    12601 atggtgagtg taactgatgt agaagttaca aatgacctaa gatatgctta tgtatatgta
    12661 agtattctgg gtggagatga agaatccaca ctaacaggtc ttaaaagtgc tggaggatat
    12721 ataagaagag aagtaggaaa aaatataaaa cttagatata ttcctgagat agtatttaaa
    12781 cttgatgatt caatagaaaa aggaatgtat atggatagcc ttataaaaag agttaatgaa
    12841 aaaaatgctc aacaaaatga ggatgaaaat tatgatgaat aatatggata gtattataaa
    12901 ctatatttat gagggtgact attttatagt tacctctcat atcagtccag atggagacaa
    12961 tattggttct acactttcaa tgtattatac attgaagaat ttaggaaaaa atgtatatta
    13021 tgtacttgat gatgaagctc cattaaattt aaggtttttg gttgaaggtg taaaaatcta
    13081 taagtctaat gaatttaata tgaagaacta ttctctaatt gcacttgatt gtggggataa
    13141 gatgagaatc tgtgtgagtg atgaaatcaa aaataatgca tcaaaattaa tctgtattga
    13201 ccatcatgct agtaatgatt catatggaga tttaaattat gtagatattg atgcatcttc
    13261 tacatgtgaa ttagtttata atttattggt tagatttcag caactaaaag atattaatat
    13321 tataaatgaa gatatagcaa catgtttata tacaggtctt gtaactgata ctggaaactt
    13381 ttcgtattca aatgtacatg caagtagctt tgaaatggca aaaaatttac ttgttttagg
    13441 tgcacaaaaa aatactataa tacaaaatat atatcaaagc aattcttctg attattacaa
    13501 attattagga gaagctctaa agggtttgga gatatttgat taaaaaatag ttacgcacac
    13561 tcgtgacttt agtcatgagt tagtagctaa aatagtcagc atatagggaa acctatatgt
    13621 agagatagga tagaacctat ccaatactac ttgaattgct ggaaacccct aaagctaacc
    13681 aaactaaaac ataaggatga aataaaccta aatgtgaaag ttacgaaagt agaaaaaatt
    13741 ggttagatgg tataaggtta aaccctaagt actttaaaat gggcaatcag caggtaagct
    13801 ccgaatagga gaaacttcaa cgactattcc tcttgaggga agtacactac aagctattgg
    13861 tagtggaagt gggtagacct taacggataa tgccgaagga taagatatag tctgtgcttg
    13921 catgaaagtg taagaagttc ataagagaac tgcataggta gtagcgaact tatgtgaacg
    13981 acaacctcaa aaatgataaa agttaagact tacggttctt aaatatgtta tttaaaatta
    14041 aatataaaac cttctaaata attgttttaa ataattacat ttgttaatat tatgataggt
    14101 gattaataaa ttgttcacca cagcattata taatatatta aatattaaaa tcattaaaaa
    14161 gtgtatcaaa aaacaattaa agtgaggtga tagcagtgaa aaaagcttat aagtttagaa
    14221 tgtatccaaa taaaaaacag caagagttaa ttaacaaaac ttttggatgt tgtagatttg
    14281 tatataataa atatcttgct aaaagaatag atgtttataa aagcgataaa gaaactttta
    14341 cttataaaca gtgtagttct gatttgacca atttaaaaaa agaattaaaa tggcttaagg
    14401 aacctgacaa attttcacta caaaacgcct taaaagattt agataatgca tataaaaaat
    14461 tctttaaaga aaaggcagga tttcctaaat ttaaatcaaa gaaaattaat agattttcat
    14521 ataaaactaa ctttacaaat ggaaacatta tgtattgtgg tcaatatata aaactaccta
    14581 aacttggtat ggttaagata agggataaac aagtacctaa agggagaata cttaatgcta
    14641 ctatatctaa agaaccaagt ggtagatatt atgtatcatt atgctgtact gatgtagata
    14701 ttgaagtatt tgaaaatact aataatcata taggtttaga tttaggaatt aaagaatttt
    14761 gtatctcaag ttgtggagac tttatagaaa atcctaagta tcttaagaag tcattaaaca
    14821 agcttgctaa attacaaaga gaattatcga gaaaaacaat cggtagttta aatagaaata
    14881 aagcaagatt aaaagttgca ggacttcaag aacatatagc taatcaaaga aatgattttc
    14941 tacaaaaact atctactaaa ttaataaaag agaatgatat tatttgcata gaagatttac
    15001 aagtaaagaa tatgattaga aatcgtaaac tatctcgctt aatttcagat gtatcatggt
    15061 cagaatttat tcgtcaatta gaatacaagg ctaattggta tggtagaaaa attgtaaagg
    15121 taggtaaatt ctttgcaagt tcacaaatat gcaataaatg tgggtataaa aacgaggaag
    15181 ttaaggattt aaatataaga gaatggattt gtcctagttg taatgaaact cacgatagag
    15241 atataaatgc aagtataaat atattaaaag aaggattaag actaataacg attcaaaata
    15301 aataacttat gtaagaaccg taggaactac ggggatagcc tgtggagaat tagtaagaca
    15361 tattttaata tgcaaaattc tgtgaagcag gaaccctgtg actttagtca tgggaggttc
    15421 agtcaaaggt atctagtata gttttaactc aagacatgat gaatagaaat aatattagct
    15481 ttaatgatgt tgatggaata acaacatata caagagacat aaaaggtata gaagttggaa
    15541 ttttatttaa agagaaaaaa caaaatgaaa ttaaagtgag ttttaggtca aaaaattatg
    15601 ttgatgtaag tgaaatagcc aagttatttg gtggtggtgg tcatgtaagg gctgctggat
    15661 gtactattag agatagtatt gataatgcaa agaaaatggt actagaggca gtgttaaaat
    15721 caatttaagg tgacagtaaa atgaataaga ttataagtat attgaaaccc acaggtatga
    15781 catctcatga tgttgtaagt agagtgagaa agattttaaa tataaaaaaa gttggtcata
    15841 caggtactct tgacccagat gcttcaggtg ttttaccaat ctgtataggg aaagctacaa
    15901 aagtatgtga cgttatttta aacaaagata aatcgtatat atgtgagctt actttaggta
    15961 taagtacaga tacttatgat gcttcaggtg aaattttaaa aaaagttgat gattttaaat
    16021 ttagtaatga agacatagaa agagcttttg atacacaaag aggagagata aatcaacttc
    16081 ctccaattta ttcagcttta aaggtaaatg gaaagagaat gtgtgattta gtaagaagtg
    16141 gtagacaatc tgaaattact ttaaaaacaa gaagagtaaa tataaaagac ataaaaatac
    16201 taagtataaa aggtaataaa gtaatgtttt atgttgagtg ttctaaaggg acttatgtga
    16261 gaagtatttg ccatgatata ggagaatatc taggttgtgg agctcatatg tcgtttttga
    16321 atagaacatc ttctggtaaa tttgacttag ataactcaat aacattagaa gaattagaat
    16381 tattttatga aaataaaact ttagacaaat acttatatga tatagattat gtccttgatt
    16441 cgtttaatta tgttgtatta aaccctaatg ctataaaata ttatagtaat ggtggctcaa
    16501 tagatgataa aaggttttta aaaaataatt tcgataaaga tgatgaattt gtaagagtat
    16561 acagtacaga taatttctta ggattaggaa agttatcaaa acacaataat acaataagtg
    16621 ttaaaagtga taagatgttc atataaccaa tgtatttaaa aaaacaacaa aagagatagt
    16681 actataataa cttctatagt ctatctcttt tacttatgct ataatagttt gtgaataagt
    16741 taagagtaaa ttatatttta gcggggaaca tttattatgg tgattataaa atctataaaa
    16801 gaaatagcaa acataaaaga aagtgtcatt accataggaa attttgatgg tgtacacaaa
    16861 ggacatcaag ttcttatagg aaaaacagtt gaacatgcaa aaaaagaaaa tatacagagt
    16921 attgtattta catttgcaaa tcatcctgta aattacttta gaccaaactc aacaaaaaat
    16981 attataagca atgatgataa aataaaatta ttagaagatt tgggaataga tatagttgta
    17041 atcataccat ttgatgagta tatgactaaa atacctgcaa aggatttcgt agatgagata
    17101 ctagtaaaaa aattaaaagc taaaaagata gtaattggat atgactttac atttgcaagg
    17161 gcaaaagaag gcaatgttaa tctattaaaa agcttagaac atgaatttgg ttttgaggta
    17221 gaagtaataa aacccatcaa aatcaatgat gtaagagtga gtagtactta tattagaagt
    17281 ttagtatccc agggtgatat ggctaatgtc aaggaatatt tgggtagaaa ttacaagtta
    17341 gaaggttgtg ttatacattc taaacacctt ggaagaacta taggattccc aacggcaaat
    17401 attgacctta agaataatat gctcgttcca aagagaggta tttatgcttc tattgtacat
    17461 attggagatg agacttattt tggtgctaca aatgtaggct ataacccaac agttaatgga
    17521 aaaactttgt ctattgaaac aaatatttta gagtttgata gagatattta tggggaaaat
    17581 ataattgttg aatttttgga aagaataaga gatgaaagaa agtttaattc aatagatgac
    17641 ttaaaaaatc aattgtataa tgatacaaat tatgtgtatg aaaactatgt ttgcaaaaac
    17701 aagtaatgta tgataaaatg attaaggaaa ataaccatta ctcggcattt gaatactcca
    17761 atcaatgctt agtaataggc gtttctaaaa aaatacggag gtaatctaaa tgataaacaa
    17821 atcagaaata ataaaagaat atggaagagc tgaaggggat actggttctc cagaagtaca
    17881 aatagcttta ttaacagcta gaataaacga gttaaatgga catttaaaaa ctcataagaa
    17941 agaccaccat tcaagaagag gactattaaa aatggttggt agaagaagaa acttattagc
    18001 ttacttaaag gaaaaagatc ttgaaggata tagagcatta atagctaaat taggattaag
    18061 aaagtaattt cattctttat aaaaaagcag gtatagtgta tacctgcttt tttatagtta
    18121 attcattata aagtaaaata gttaataata aaagtagttt atatgttata aaaaattata
    18181 aaatgaataa tattgggtaa gaatattaaa atagtataca aatacataat caatttaaaa
    18241 ggaaagacag gataaatata gaaattttat aacaggataa ttagttggaa taaggaggat
    18301 attattttga aaataaagtt agtatgcgat agtttgtgcg atattccaga tgaaatatca
    18361 gaaaaagatt atatagagat agttccactt acagttattt ttaatgatag ggaatatata
    18421 gaaggagtag atataaagaa agaagagttt tataaaaaag taaaagaaat caagcaaata
    18481 cctaaaactt cacaagctac atatatggag tttaaagaag tgtttgataa gttcataaca
    18541 gaaggttatc atataatctg tatgacaggg gctgcaaacg catcaggaac ttttcaaagt
    18601 gctatgatag ctaaaaatga tgtaaatgaa aatgaaaaaa tacatatatt tgatactaga
    18661 aatttatctc taggtagtgg acaatatgtt ataaaggcat gtgaactttt tgaagaagga
    18721 ctgggatttg aagaaattat agatgaatta gaaaatactc gcagtagtgt aagattgctt
    18781 tttgcaccat atacacttga ttttttaaag cagagtggaa gagttccagt tgcaactgct
    18841 ttgataggaa atatgttaaa tataaagcct atatttttct ttgataatgg ggaagctaag
    18901 ttggtaaata aggttagagg aataaagaat atagcaagta aattggtcga tataatttta
    18961 gaaatgaatg aagggtcttt agaaggaaaa atcgtcacaa taggttgtgg agataattta
    19021 cacgattgtg aaatattaaa agatgaagta aataaaagaa taaaagcaag aagagtatta
    19081 tttactcgag gaggagtttc tatttgctct catacagggc cagatatatt ggctataagt
    19141 tgttctaatt agtagtaata taaggttttt gcatatatat gtgaagataa ttttatattg
    19201 ttaatttata gtatttttga taatatagta aatagagtga tatacgtgtt ttttaataag
    19261 aggaggacaa gcattaatgt ttgaacataa aatatttaaa atggattttg caggaagaga
    19321 actttctgta gaaataggaa agatttgtga aatggctagt ggtagttgta tagttcgata
    19381 cagtgatagt atggtaatgg taaatacaac taaatcagcc aaacctagag acggaataga
    19441 cttttttcct ttaagtgtgg attatgaaga aaaattatac tctgtaggta agatacctgg
    19501 aggtttttta aagagagaag gcaagccttc agagaaagct atacttacat caagattgat
    19561 agatagacca ataagacctt tattcccaaa aggatttaga aatgatgttc aggtagttgc
    19621 aacagtttta tcagtagacc aagattgtac accagatata gtagcaatga taggttcttc
    19681 aatagccctt tctatatcag acataccatt taatggacca acaggttcag tatgtgttgg
    19741 ccttgtagat ggtgcatttg ttgtaaatcc aaacgcagag caaagagaaa aaagttcaat
    19801 gcatctagtt gtttcaggta caaaggaagc aataatgatg gttgaagccg gagctgatga
    19861 agttccagac gaggtaatgc ttgatgctat tttatttgct caccaagaaa taaaaaaaat
    19921 agttgagttt atagaaggta tagttgcaga agttggaaaa gaaaaaatgc ctgttgaatt
    19981 ataccatgca ggagaagaaa taactcaatt ggttcgcgaa tttgcaactg ataagatgaa
    20041 aaaagcagtt cagacttttg aaaagttaga gagaatggaa aatatggata gagttaaaga
    20101 agaaacttta gctcactttg aagaaacttt agaagatttt gaagattttg ttggagatat
    20161 agaagaagta ttacaagata taataaaaga agaagtaaga aaacttatag tacatgaaaa
    20221 tgtaagacca gataatagaa aacttgaaga aataagacct atatggtgtg aaactggcat
    20281 gatacctaga gctcatggtt cagccatatt tactagagga caaactcaag tacttaatgt
    20341 tgctacttta ggtgctttag gagatgttca aaaactagat gggttagatg aagaagaaaa
    20401 caagagatat atgcatcatt ataatttccc tgcatatagt gttggagaag ctagaccatc
    20461 aagaggacca ggaagaagag aaataggaca tggtgcttta gcagaaagag ctttattacc
    20521 agtaatacca tctcaagaag aatttcctta tgctattaga ttagtttctg aagtattaag
    20581 ttcaaatggt tctacatctc aagcaagtgt atgtggctca acattatctt tacttgatgc
    20641 aggtgtacct ataaaagata tggttgcagg tatagcaatg ggacttataa agcatgatgg
    20701 taaggttgca gtgctttcag atatacaagg tatggaagac catttaggcg atatggactt
    20761 taaagtagct ggaacagagt atggtataac agcaatacaa atggatataa aaattgatgg
    20821 tatagataaa gaaattctac aacgtgcttt aaaacaagct aaagagggta gaatccatat
    20881 actaggagag atgagaaaaa ctatatctca gccaaaacca gaactttcac catatgcacc
    20941 taagattgtt aaaatgcaaa taaatcctga caagataaaa gatgtaatag gacctggtgg
    21001 aaagataata actaagataa tagacgaaac tggagttaag atagatattg aacaaactgg
    21061 ggaagtattt atatcaggta ttgagattga tatgattaag aaagctcaag aacttataaa
    21121 taatatagta gttgaaccag aagtaggaaa aacttataaa ggtaaggtaa gtagaataat
    21181 gaactttgga gcatttgttg agatacttcc aggaaaagaa ggattacttc atatatctca
    21241 tatagctcat gaaagagttg ccaaagttga agatgtattg aatatagggg atgaagttga
    21301 agttaaagtt acagagattg atgaaaaagg aagagttaat ttatcaagaa aagttctttt
    21361 accaaaacca gaacataaaa ataaataact tattatataa ttaaaagcaa agtcttagga
    21421 ttttgctttt ttaaatacaa aaaagtagat aattttaaaa tgatagttat ggatggctta
    21481 tagttctttt tctgtcaggc tttactctta taaagaagct atttaaaagc tgattaatag
    21541 agagctttat tgatatatat agaatcactt aagttatatt tagtgcataa acagagctta
    21601 tttttaatag tattaaataa gaaacaattt gtacataatt agggggagtt ttaatgataa
    21661 tgatggtact taataagagt aagctcaaaa aaatcataat actaatttta gtagtgttat
    21721 tagtattatc aggaggggta atggtattaa agttatttaa caacaagcca gtatttaatt
    21781 tatttaatgg tgtagattta ccatatgaaa gaggtacaaa ggataaaagt ggatatgttg
    21841 cattaacctg taatatagac cttggatggg aaacagagta cgtagaatcc attttagaaa
    21901 cacttaaaaa agaaaatgta aagattacat ttaatgtaac tggaaaatgg gcagaaaaga
    21961 ataaggatga attattaaag ataaaaaaac aaggacatga aataggtaat catggataca
    22021 aacatttaga ttattctaca ttatcatatg aagacaatta tgagcaaata gagacctcta
    22081 aaaagataat tgaagaaatt ataggtgaaa aaactaaatt ttttcaagca ccagcaggtt
    22141 cttttggtcc agaaacagta aaggctgcta aagcattagg gtatacttct atcaaatggg
    22201 atgccgatac tatagattgg aaatacaaag accaaccaga ggtaatcatt gatagaatga
    22261 agaaaaaaga cattaaagat agtagtataa ttttaatgca tccaaccaac gcaacgacaa
    22321 aatgtataga tgatattata gctattgtaa gagaaaaagg actaaaacca ggaaaactta
    22381 gtgatgtatt taagtagaca aagtataaat atttggttaa gattgtataa gttgtagttg
    22441 aattaattaa aagactgact tataatgtaa tacatataaa aaataaatat aattaaaaca
    22501 tagtgttttc aataatccaa tatgaaacaa agaataaatt gttaggagaa tgtaatgtat
    22561 aagacaaaaa tactggaaaa tgggcttaca ataattggtg aagaaatacc atatctaaag
    22621 tcaataacat taggtatttg gataaatgca ggctctagga tagaagaagc acaggtgagt
    22681 ggaacttctc attttataga gcatatgatg tttaaaggaa ccaaaaacag aacatcaaaa
    22741 gagattgcta gttcaataga taatttgggt ggtcaaataa atgcatttac aagtaaagaa
    22801 tgtacatgtt attatgtaaa actaatagat gagcatatag atacaggaat tgatgtattg
    22861 agtgatatga tactaaattc taaatttgat aaaaatgata tagataagga aagacttata
    22921 atattggaag aacttaagat gtatgaagac tctccagatg acttatctta tgatttatta
    22981 gtggaaaata tatatgctaa tgatggatta ggaatgaata taattgggac aaaagaatct
    23041 ctatacaata taactagaga atccatgtta gaatacttaa ataagtatta tattccaaat
    23101 aacgctgtta tttctatagc tggaaacttt aactttgatg atatggtgga gaaaatcaaa
    23161 tcaaaatttg gacactggga aaagaaaaat ttaagtatag acataagtga ggctaagttt
    23221 aatccttgct ttatatcaaa aaataaagat actgaacagg tgaacttagc tatgtgccta
    23281 aaaggtattc cttttgaaaa tgatgaagaa gtctattcaa tggcagtggt aaataatatt
    23341 ttcggtggaa gtataagctc aaggcttttt cagaaaataa gggaagaaaa aggtttagta
    23401 tattcaatat actcatcaca gacattatat aggaaatgtg gtgaattagg tatatttgca
    23461 agcatgagta caaaaaattt acaagatgta tataatttaa taaagaaaga aattgaaaat
    23521 ataagagaaa actatcttac agaaaaagaa atatctgaaa gcaaggaaca gttaaaagga
    23581 aattatatat tggatttaga aagtactagc agtagaatga tgtctactgg taaatctatg
    23641 ttacttagta aaaaagtaaa aactacagat gaaatacttg aatgtataaa taatgttaat
    23701 attaattcaa taaagaaggt tgttgataag gtattcaata tcgaaaatat tggtacatgt
    23761 atagttggaa gagatgtaga aaagattcta cacttggatt aatattgaaa aaagtaaatc
    23821 tctggaggta taaaatatga atttatctga aatagcaggt aaggaaatag ttaacttggt
    23881 cacaggagaa agattggggg tcattggaga atgtgacctt atactagatg aaactacagg
    23941 cggtatatta gcgcttctaa ttccaaaaga aaaaggattt tttgccagaa gaaaagataa
    24001 gtcattttta gaagtaccat ggagaaatgt aaaaaaaata ggtaatgaca tgataattat
    24061 agaatatgag ggatatattt aggaggaaaa aatgagtatt ttagtgcaaa agtttggtgg
    24121 gacatcagta gaatcatatg aaaaaatgaa tgaagtatgt aagatagtaa aagcatacaa
    24181 aaaaaatgat gaagaattac aattagtatt agtagtttca gcaatgggaa gaaaaggagc
    24241 tccatatgct acagatacac ttataaattt gtgtagtgca gtaaatgatg agccaagtaa
    24301 aagagaactt gatttaataa tgtcttgtgg agaaataata tcaggaacaa ttcttgctaa
    24361 tttattgaat gcacagggga tagagtctgt tttctttact ggtcagcaag caggtataat
    24421 tacaagtgat gaatattcta atgcaaagat aaagtatata aatcctaaaa aggtaaaaag
    24481 agctttagat gatggtaagg ttgtagttat agctgggttc cagggtgtaa cagatgatgg
    24541 agaaataact actcttggaa gaggcggaag cgatacatca gcagttgcta ttggtaaagc
    24601 tttagaatgt gaaacagttg aaatttacac tgatgtggat ggtataatga ctgcagaccc
    24661 tagagtagaa ccaaacgcta aagtattgag ttttatagac tatgaggaag tattccaaat
    24721 ggctgacaaa ggtgcaaaag taatacatcc aagagcagta cagttggcta aaagtggaaa
    24781 tataacttta gccataaaga atacaatgaa tccaactttt gaaggtacaa aaataggttc
    24841 tttatgtaga cacttagaag acaatataga atatgaacaa gaaagagatt ttaaggttgc
    24901 tgttgcaaat aaagatagcg tagcacaagt taagattaag tcagcagaag aagtgtttac
    24961 agaagtatta aatgagatag aaaagaaact tataactata gatatgataa atttctttat
    25021 atcagaaaaa gcatttgttg tagaagatgc tgatataaaa tcattaaagg aaatactaga
    25081 taaatttgaa ttagattatg aagtaaatag agactgtgct aaggtaacat tgatatgttc
    25141 aagaattgct gatgaaatgt ctggtatcat gtcaaaggta gttagaggat tatcaaaagc
    25201 aggggtatct cttttacaaa catcagactc aaatatgact atatcttgtt tagttagtga
    25261 agaagatatg catacagcag tacatgctat acatcaacaa ttttatttaa aataaactgt
    25321 ataatacaat tattttttaa tcaaatttaa gaatttcata tatatcttaa tatatttgat
    25381 ttatttggta aaactaaatt aaatataatt ttgataggag tttttttatg aaagattata
    25441 gagaagataa tgaaagattt gataaagata attgtagtga agaatctagt tgtgaagaga
    25501 gagaatgtat aaagcaattt ggtaccaatg aaatgccacc tcaacctcct aaagatattc
    25561 aatgtataac tataattgga gaaattgagg gacattttat aggaaatcct caaaaaaaag
    25621 ctacaaagta cgagcatata attccaatgc tatactcaat tgaggaaagt aatgatgtta
    25681 aaggagtgtt agttgtttta aacactgttg gaggagatat tgaagcaggt ttagctattg
    25741 cagaactttt aaacagtaca tctaaaaaag tagttactct tgttctagga ggaagtcata
    25801 gtataggtgt tcctcttgct acagcaggag attactcatt tatagcacca acagcaacca
    25861 tgattataca tccagtgcga actactggtc ttgtgattgg tataaatgag acttttgaat
    25921 attttaaaaa gatgcaagat agaataattc aatttataat aagaacatca aatatcaaaa
    25981 aagatgtact tgaaaaactt atgcatgaaa aagatgaact tgtaagtgat gttggtagtg
    26041 tattaatagg aaaagaagct gtggattatg gtcttataga tgaagttgga ggactaaaag
    26101 aagcacttaa aaaattaaga gagcttataa aagaatctga ggaagaaaaa aataatgatt
    26161 aaaatttgtt taaataataa aatttatgat ataatgatgt aataaagtag gagtgttagt
    26221 gttaaggcat tagcactttt tgctgtgaag gggtaaaact tgctaaaaat aaggagtgat
    26281 attaatggcc acgaaaaaga agaaaaaaaa gaaggtaaat accttaagtt ttaatgcaga
    26341 atatcataat ttaataacaa tatttatagg tgtattttta ttatatagtt taaattctaa
    26401 ttctatggga tggatacctg tgttaatgca aaatcttttt aagggactat ttggagggct
    26461 ttcaatagca ataccattta tagtaattat aacaggactt ttaggatttt ttgatggaaa
    26521 tgaatatata tataggctaa gaaagactaa actatactac ataattattt tatttatatt
    26581 tgtattttat ggattattaa atgcaggaac tcttcctgta gatagcccac taaaaggtaa
    26641 catgtttgat gatgttatga aattgggtgt aagtggacaa ggtagtggtt tgatagcaac
    26701 aactattgcg tattatatga gtaaaatatt tggtatagca ggtggatggt taataagtat
    26761 cttcgcactt atactttctg taatgtttat atttaatatt tctataaaag atttggtgtc
    26821 aaatgcaaaa tcaaaatcta aagcatctaa agacagcaat ttaacgttta aagataaaat
    26881 tgcaaatatg aaaaaatctg caatagatat gatgactgat gaagtagatg atactactat
    26941 aaataaaaaa ccaggtttct ttaaagggtt aatgtcaaaa ggtcttaata atgatgatga
    27001 tgaagatgaa tattttgaag aagaaaatag tgatggtgta gatgacaaaa ctataaaaat
    27061 agttggattt aataaggcag aagatgagta tttagaaata ttagaaggaa ctcaaagtat
    27121 gccagagtta gatgttttaa aagaattaca aaaagctacc aatgaaaatc cagttgtaga
    27181 tactaaagca gaaaaaaaag ttgatatagc taaacctaat ttaaatatag aaaaaactca
    27241 acctatgagt attgtagcag agccagttaa tgaggattat tcaaactata aaaagcctag
    27301 cattgagctt ttaaataaag taaataagaa gtctgatgaa aatggtaaaa agaaagtgtt
    27361 gaaaaatgct tctcttttag aaaagacact atcagatttt ggagtggaag caaaaattaa
    27421 tcaagttaca gtcggaccta ctataacaag atatgaaata caaccaagtc caggagtcaa
    27481 ggtaagtaaa attgtaaatt taactgatga tatagcttta agtttagcag ctaaaagtat
    27541 aagaattgaa gccccaattc ctggtaaaag tgcaattgga atagaagttc caaatgaaga
    27601 agctcaaatg gttggtgtga gagaagttat agaaagtgaa gaatttaata attttaactc
    27661 tcctcttgca atgggacttg gaaaagacgt agcaggaaaa attataattg gagatatagg
    27721 taagatgcca cacttactaa tagctggttc tacaggttca ggaaagagtg tgtgtgtaaa
    27781 tacattaata agtagtattt tatataaggc aaatcctgat gaagtaaaat tactattaat
    27841 agacccaaaa gtagttgaac ttgcaaatta taatggaata ccacatctat tgataccagt
    27901 agttactgac cctaaaaaag ctgcaaatgc tttaaattgg gcagttacag agatgaatag
    27961 aaggtacaaa ttatttgcag atgctcaagt aaaagatgta acaagttata atgagaaagc
    28021 ggaagaaaaa cttcctaaaa tagtaataat aatagatgag ttagcagatt taatgatggc
    28081 aagtgcaaat gatgtagaag attatatttg tagattagct caaatggcaa gagctgctgg
    28141 aatgcacttg attgtagcaa ctcaaagacc atctgttgac gttataacag gggtaataaa
    28201 ggctaatata ccatctagaa tagcatttgc agtatcatct caaactgatt caagaacaat
    28261 ccttgatatg ggtggtgcag agaagcttct aggtaaaggt gatatgttat tctatccact
    28321 tggtgcagca aaaccagtta gacttcaagg ggcattcata tcagagagtg agtcggaaaa
    28381 agtaatcgat tttgtaaaaa gtcaagttaa agatggaata aaatatgaag aagatataat
    28441 tgaaactata tctaaggtta atacttcaaa gggtagtgat gaggatgagt ttttgagtga
    28501 agcgatagaa tttgtcgtgg aaagtggtca agcatctgcc tctatgctac agagaagatt
    28561 taaaatagga ttcaatagag cagctagact tatagattct atggaagaaa gaggaataat
    28621 tggagcaagt gagggaagta aaccgagaaa ggtattaata agcaaacagg atttgcagaa
    28681 tttagaaggt gaatagaatt gtcagttata aaaatagaaa aagcacttaa gctagataaa
    28741 gtaatatttg tagatgtgag aactgaagga gaatatgaag aagaccatat attgaatgca
    28801 atcaatatgc ctctttttaa aaataatgaa cataatgaag ttggaacaat ttataagatg
    28861 cagggaaaac atgaagcaat acaaaaaggg tttgactatg tatcgtataa attaaaggat
    28921 atatatttac aagcagcaga acttgcatta aattatgata atattgtcat atattgtgca
    28981 agaggcggaa tgagaagtgg cagtatagta aatttattat catcattagg tgtaaatgta
    29041 tatcaacttg aaggtggata taaggcttat agaaattttg tattagagta tctaaggcat
    29101 gtaatggata caaaaaactt tgtatctgtt catggattga ctggtacagg aaagactgat
    29161 ttgttacatt tattagatga aaaaggaata gacgtactgg atttagaagg catagctaaa
    29221 aacagtggtt ctacctttgg ttttataact tttgataaaa agccaccatc tcaaaagaac
    29281 tttgaaacaa aactatttga aagtatattt ttttcgaaag agaattatat atttgtcgag
    29341 agtgaaagca aacgagttgg aagtgttaca gttccaaatg aaattttcga ggcaatgaca
    29401 cgagaaggac atcatatatt attagaatgt gatattgaaa atagagtcga tagactttgt
    29461 agagattata tttatgataa agatgaaggt aatatattga ttttaaaaga atgtataaac
    29521 aagtttagaa aaagattggg taataaagta gtagatgatt atattgattt attagaatca
    29581 ggtgaatata aagaacttgt aaaaagatat cttttagaat actacgaccc actttatatg
    29641 cattcagtag agaaatataa gtatagtaag attataaatt ttaatgatac acaaaaagct
    29701 ttagaagatg ttatagactt atatgagagt atattggaag gagaaagtga atgttaaaaa
    29761 tagcattgga atcattagga tgctcaaaaa atttagtaga tgcagaaata atgatgggaa
    29821 ttttaaataa caaaggctat aaattaattg gagattttga agaagctgat gtaattatag
    29881 taaatacatg tgggtttata gagtcagcta aacaagaatc tatagatacg ataataaact
    29941 ttgcagagtt aaaaaaaact ggcaatttaa agttgttgat agtaacaggg tgtctagccc
    30001 aaagatattc tgaagaatta aaaactgaaa ttcctgaaat agatgctata gtaggaacag
    30061 gaagctatca aaatatagat aaaatactta aagaattaag tgaaatgcat caaattgtaa
    30121 gccttaatga tatagagttt gtatttaatg aagatttacc aagatatata tctacaccta
    30181 gctatatggc ctatttaaaa attggagaag gatgctccaa taattgtact tattgtataa
    30241 taccaaaact aagaggtaaa tatagaagta gaaaatttga agatataatt aaagaagcta
    30301 agaaattagc tgaatctgga gtaaaagaac ttgtggttat agcacaggat acgacaaagt
    30361 acggttttga tttatatgga aaagaaagat tatcagaact tttagaagaa cttgcaaaaa
    30421 tagatggatt taagtggata agggttatgt attcatatcc agaatctata acagaagaat
    30481 tgatacaagt tataaagaaa tatgataata tatgtagtta ctttgatatg ccaatacagc
    30541 atgcaagtaa caatatttta aaattaatga atagaaaaac tactaaagaa gatatattaa
    30601 ataaaatcaa tctgataaga agtaatatac cagatgctat acttagaact acaataatag
    30661 tagggtttcc tggtgaaaca gaagatgact tcaaacaact tgtggacttt gttgaagagg
    30721 ttaaatttga tagacttggt gcatttgcat attcaagaga agaagataca ccagcagata
    30781 gattgccaaa ccatatagat gaagaagtaa aaatacaaag aagagatact ttaatgatga
    30841 ttcaacaaaa aatatctgaa gaacttaatg acaaaaagat aggaaaaact tatgaggtgt
    30901 taatagaaga acagatagaa gataatgtat atacaggaag aacacaaggt gatgcagaag
    30961 aaattgacag tatagtgtat gtaaaaagtg tagacaatct tgaagtagga gaatttgtaa
    31021 gtgttcaaat aaatgatgca atggaatacg atttaatggg agatgtttta tatgaacttg
    31081 cctaacaagt taactttgtt tagaatattt ttaataccag tatttgtttt aattatgttg
    31141 ctcaatatac caaataaatt tttaatagct tgtattattt ttgtaatagc ttcaattact
    31201 gatgctttag atggaaaaat agctagaaaa tacaatctag tcactgactt tggaaaattt
    31261 atggacccat tagcagataa gttattggtt atttcagcac ttacatgtat gatagaagac
    31321 catcttgtaa gtagttggat ggtaattata atagtggcta gagagcttac agttagcata
    31381 ttaagagcta tagctgctgc tgatggaaaa gtaattgctg ctggaaacag tggaaaactt
    31441 aaaacgataa ctcaaatggt atctatagtg tttttattgt taggtgctca gtttgaaaat
    31501 gtattgatat taaatattgg tgaaattctc attcttatag ctacactact tacattatat
    31561 tctggatggg aatatctata taaaaataaa gagcttttta tgtcttctaa gtaaaaaatt
    31621 atgctttaat aaataaaaac taatgattaa taaataaaaa ataacaactt aaaattttaa
    31681 aaatgtaaaa aagggtatac tattatagta tacctctttt cattttaaga atattttctt
    31741 ttgtgtatat aattattaat aatggtttat aaataaaatg taatgaataa aaatataatc
    31801 attagtagcg tttttaaatt gactaggttt gatttatgtt atataattaa tatataacaa
    31861 gagaacaaat gtttgtagat aaatgaaggg atggtataga gatgagtgta gatcaagaaa
    31921 aattaaaagc gttgaatgaa gctttaggta aaattgaaaa agattttggt aaaggttcag
    31981 taatgaaatt gggagaagca acatctatgt ctatagatgt tatatcaaca ggagcgattg
    32041 gtttagacat agctattggt ataggaggtc tacctagagg gagaatagtt gaagtatatg
    32101 gtccagaatc ttctggtaag actactgttg cgcttagttg tgtagcatca gctcaaaaag
    32161 atggaggaat agctgcattt atagatgcag aacatgcact tgacccagta tatgcaaaag
    32221 ctttgggtgt ggatgttgat aacctaataa tatctcaacc agatacaggt gaacaggctt
    32281 tagagatagc agaggcattg ataagaagtg gagcgataga tataatagta atagactcag
    32341 tagcagcatt agttccaaag gctgaaatag atggagatat gggtgattct catgtaggtt
    32401 tacaagctag acttatgtca caagcactta gaaagttaac tggttcaatt aaaaaatcaa
    32461 attgtgttgc tatatttata aaccagttaa gagagaaagt aggaataatg tttggaaacc
    32521 cagaaactac tactggagga cgtgcactaa aattctattc atcagttaga ttggatgtta
    32581 gaaaaataga tacaataaaa caaggtgata aagttatagg tagtagaact agagttaaag
    32641 ttgttaaaaa caaagtagca ccaccattta agcaagctga atttgatata atgtatggag
    32701 aagggatttc aaaaattgga gaccttttag atatagctgc tgatgtagat atagttaaaa
    32761 aatcaggttc atggtatagt tacaatgata ctaaacttgg acaaggaaga gaaaatgtta
    32821 aaaaattctt ggaagataat ttagatttaa ctactgaaat agatgagaaa gttagagcat
    32881 tttacaattt aaatgaagaa catgaagaat caggtacttc agtatcaaaa gaaattgtag
    32941 aagaataagt aaaaatacaa aaaaataact agtaattaaa gacatgccac gctccctatt
    33001 agcttgacac aatgttgaaa aaattataaa attaagtgag gataaaatat ctaaatttaa
    33061 ttttaattat taggaatatt ttttaatcat agtgatgaca ttttgggatt attaacgaag
    33121 gaggtggatg tcatagatag tgtagtaatg attgtaatag gtgcagctgt tggtattata
    33181 gctggatatt ttgttagaaa aaatatatct gaagcaaaaa ttggacaagc tgaaaacttg
    33241 gccaaagaaa taatagataa agcacaccac gactcagaaa cagtacaaaa agaaaaatta
    33301 ctagaagcta aagaagagat ccataagtgg agaacagaag ctgaaagaga aaacagagaa
    33361 agaagaacag aagtacaaaa gtatgaaaaa agagtagtac aaaaagaaga agtcttagat
    33421 agaaaattac aaaatttaga atctaaagaa gtaaacctaa gtgaaaaatt aaaggtagtg
    33481 gaaaagaaag aagaagaagt tgaagtaata aaaactcaac agttagaaaa attagaaagt
    33541 atatcaggaa ttacttctga taaggccaaa gaaatcatct taaccaatgc ggagagagat
    33601 gtaagacgtg aaatgtctat aatgataaaa gagatagaaa gtcaagctaa agaagaagct
    33661 gataagaaat caagagaaat tataggatat gcaattcaaa aatgtgcagc agatcacgta
    33721 gccgaaacta ctgtaacagt agtaagcttg cctaatgatg aaatgaaagg tagaataata
    33781 ggtagggaag gtagaaatat aaggacttta gaaaccttaa caggtataga ccttataata
    33841 gatgacactc cagaagccgt aattttatca ggatttgatc caataagaag agaaattgca
    33901 agaattgctc tagaaaaatt aatagcagat ggaagaatac atccagctag aattgaagaa
    33961 atggttgaaa aggcgagaaa agaagttgac aatattataa aagaatatgg tgaacaagct
    34021 gcatttgaaa ctagtgtaca tgggattcat ccggaattaa taagattatt aggtagactt
    34081 aactatagaa ctagttatgg acaaaatgta cttaaacatt ctatagaagt tgctcatata
    34141 gctggtataa tggctgctga aataggagca gatattagat tggctaagag ggcaggttta
    34201 ttacatgata taggtaaggc tgtagaccat gaaatggaag gtacacatgt tgaaataggt
    34261 atggatttac ttagacgtta taaagagtct aaagaagtta tacatgccat gagtactcat
    34321 catggagatt acgagcctca aacagtagag gctgtattag ttacagcagc agatgcaata
    34381 tcagctgcta gacctggtgc gagaagagaa actttagaag catatataaa gagacttgaa
    34441 aaattagaag aaatagctaa ttcatatgaa ggtgtagaaa aatcatttgc tatacaagct
    34501 ggtagagaaa taagaataat ggtaaaacca gaagctataa atgatgaaga aatacatcta
    34561 ttggctagag atatgactaa gaaaatagag gatgaacttg aatatcctgg acaaataaaa
    34621 gtaagcataa taagagaaac aagagctata gagtatgcga agtaaaagaa ataatatcta
    34681 aataataaga aaatattttt agaaatttaa atttgccaca agatatgata atatatcttg
    34741 tggcttttaa attgtttaag tttaatttta tataatgcaa aaataatcta agtgctaaaa
    34801 gtgctactta caaaagatta ataaaataaa gctttatata tgtattattt atataattat
    34861 ccaagtttgt ttgatataat gaagttgtaa aatcaatact ttactacaaa ataaatagta
    34921 aatactacag taa
//
LOCUS       DAEMIK010000024        34247 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEMIK010000024 DAEMIK010000000
VERSION     DAEMIK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.024"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(30..1046)
                     /gene="tsaD"
                     /locus_tag="KPK87_001009"
     CDS             complement(30..1046)
                     /gene="tsaD"
                     /locus_tag="KPK87_001009"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG2537180.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREILSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(1052..1528)
                     /gene="rimI"
                     /locus_tag="KPK87_001010"
     CDS             complement(1052..1528)
                     /gene="rimI"
                     /locus_tag="KPK87_001010"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG2537181.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(1518..2234)
                     /gene="tsaB"
                     /locus_tag="KPK87_001011"
     CDS             complement(1518..2234)
                     /gene="tsaB"
                     /locus_tag="KPK87_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG2537182.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVDFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(2234..2686)
                     /gene="tsaE"
                     /locus_tag="KPK87_001012"
     CDS             complement(2234..2686)
                     /gene="tsaE"
                     /locus_tag="KPK87_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG2537183.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(2825..3865)
                     /locus_tag="KPK87_001013"
     CDS             complement(2825..3865)
                     /locus_tag="KPK87_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2537184.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMESVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(4194..4799)
                     /locus_tag="KPK87_001014"
     CDS             complement(4194..4799)
                     /locus_tag="KPK87_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2537185.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(4924..5041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5101..6267)
                     /locus_tag="KPK87_001015"
     CDS             complement(5101..6267)
                     /locus_tag="KPK87_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2537186.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEIGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(6415..8556)
                     /locus_tag="KPK87_001016"
     CDS             complement(6415..8556)
                     /locus_tag="KPK87_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG2537187.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIREI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(8700..9807)
                     /gene="prfB"
                     /locus_tag="KPK87_001017"
     CDS             complement(join(8700..9731,9733..9807))
                     /gene="prfB"
                     /locus_tag="KPK87_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG2537188.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(9822..12497)
                     /gene="secA"
                     /locus_tag="KPK87_001018"
     CDS             complement(9822..12497)
                     /gene="secA"
                     /locus_tag="KPK87_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG2537189.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(12629..13180)
                     /gene="raiA"
                     /locus_tag="KPK87_001019"
     CDS             complement(12629..13180)
                     /gene="raiA"
                     /locus_tag="KPK87_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG2537190.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(13732..14406)
                     /locus_tag="KPK87_001020"
     CDS             complement(13732..14406)
                     /locus_tag="KPK87_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG2537191.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     INIISR"
     gene            complement(14409..15461)
                     /locus_tag="KPK87_001021"
     CDS             complement(14409..15461)
                     /locus_tag="KPK87_001021"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG2537192.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(15510..16469)
                     /locus_tag="KPK87_001022"
     CDS             complement(15510..16469)
                     /locus_tag="KPK87_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG2537193.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCVATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(16565..17083)
                     /locus_tag="KPK87_001023"
     CDS             complement(16565..17083)
                     /locus_tag="KPK87_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG2537194.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(17094..18428)
                     /gene="celB"
                     /locus_tag="KPK87_001024"
     CDS             complement(17094..18428)
                     /gene="celB"
                     /locus_tag="KPK87_001024"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG2537195.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKILDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(18467..18793)
                     /locus_tag="KPK87_001025"
     CDS             complement(18467..18793)
                     /locus_tag="KPK87_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2537196.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(18817..19125)
                     /locus_tag="KPK87_001026"
     CDS             complement(18817..19125)
                     /locus_tag="KPK87_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2537197.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(19266..21179)
                     /locus_tag="KPK87_001027"
     CDS             complement(19266..21179)
                     /locus_tag="KPK87_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2537198.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(21511..22293)
                     /locus_tag="KPK87_001028"
     CDS             complement(21511..22293)
                     /locus_tag="KPK87_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG2537199.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(22296..24515)
                     /locus_tag="KPK87_001029"
     CDS             complement(22296..24515)
                     /locus_tag="KPK87_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG2537200.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(24779..25876)
                     /locus_tag="KPK87_001030"
     CDS             complement(24779..25876)
                     /locus_tag="KPK87_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537201.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(26192..27385)
                     /locus_tag="KPK87_001031"
     CDS             complement(26192..27385)
                     /locus_tag="KPK87_001031"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG2537202.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(27521..27624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            27813..28322
                     /gene="yyaC"
                     /locus_tag="KPK87_001032"
     CDS             27813..28322
                     /gene="yyaC"
                     /locus_tag="KPK87_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG2537203.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(28433..28861)
                     /gene="fabZ"
                     /locus_tag="KPK87_001033"
     CDS             complement(28433..28861)
                     /gene="fabZ"
                     /locus_tag="KPK87_001033"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG2537204.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(28877..29896)
                     /locus_tag="KPK87_001034"
     CDS             complement(28877..29896)
                     /locus_tag="KPK87_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG2537205.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(30093..30353)
                     /gene="spoIIID"
                     /locus_tag="KPK87_001035"
     CDS             complement(30093..30353)
                     /gene="spoIIID"
                     /locus_tag="KPK87_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG2537206.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(30451..31119)
                     /locus_tag="KPK87_001036"
     CDS             complement(30451..31119)
                     /locus_tag="KPK87_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG2537207.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(31185..32249)
                     /gene="spoIID"
                     /locus_tag="KPK87_001037"
     CDS             complement(31185..32249)
                     /gene="spoIID"
                     /locus_tag="KPK87_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG2537208.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(32336..33589)
                     /gene="murA"
                     /locus_tag="KPK87_001038"
     CDS             complement(32336..33589)
                     /gene="murA"
                     /locus_tag="KPK87_001038"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG2537209.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(33610..>34247)
                     /locus_tag="KPK87_001039"
     CDS             complement(33610..>34247)
                     /locus_tag="KPK87_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG2537210.1"
                     /translation="SKALEISEANFKFYNVKINSVINHNLSNKEFHNIFIEIINNLKL
                     DKKGINWNQKGSGDKIQLCATIKGSDKEISIKGINKNNKESYIVVDILDNKVYKHKEV
                     IYSNVENVLNKYSSQVDVSACIVGEYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVN
                     FLSITAYSKVLNDYELEYLGEKINLNIGIKYNEDDGKTLIYIATPIIKLDY"
BASE COUNT        10181 a         6449 c         3565 g        14052 t
ORIGIN      
        1 tacttttata tttacaaatt catctaactc tactgattta tctttaaatt tggaactgca
       61 tttaaactca tatcatgcgt tttcccgttt ataaaattat aatatcctgc acaccctatc
      121 atagctgcat tatctgtgca taaaattaga ggaggatact taacagtaat accattgtct
      181 ttagcaagct ctgttatttt agctctaagc cccgaattag aagcaacccc accagaaaga
      241 gttatgatat tgtatccttt gtcttttact gcttttagtg ctttagtaga taaaacctct
      301 actacagcct cttggaaaga tgctgctaca tcttcaacta ctatttcttc atttttcatt
      361 ctttttccat ttagataatt taatacagaa gattttaatc cactaaaact aaaatcatag
      421 ctatcttctt ctaaataggc tcttggaaat tctatagcat gtttatttcc attcttggcc
      481 agattatcaa taataggacc tcctggatag cctaaattca tagctcttga aatcttatca
      541 aatgcttctc cagatgcatc atctcttgtt tttcctaaaa tttcatactt gccataatct
      601 tttacttcaa ccaagtgagt atgacctcca gatactatta atgttataaa aggaggtttt
      661 aaatccttat gctctatata gtttgcactt agatgccctt ctatatgatt tactcctaca
      721 agtggaatat tcaaagtata tgcaagtgct ttagcataag atagaccaac caataaagca
      781 cctactagcc caggtccata tgtaactgcg atatggtcta tatcattaaa acctatattt
      841 gctttgtcta aagcttcttg aacaactata tcaatatttt ccacatgttt tcttgaggcc
      901 acttctggaa ctacaccacc aaatttttta tgtgtctcga tttgagtaga gattatgttt
      961 gaaagtattt ctctaccatt ttttaaaact gatgcagctg tttcgtcaca actactctct
     1021 atagctaatg ttattatatc actcataaac tctacacctc ttttaattgg ttccacatta
     1081 taattgcatc ttctttatta tcactgtagt attcttttct tattccaccc attttaaatc
     1141 catattttct atataaattt tgtgctattt tatttgatgc tctaacttct aatgtcatag
     1201 atgcaatatt attatcttta cataaatcaa ctatgccttt tatcaacttg tcccctattt
     1261 ttttccctct atagtcacta tgaacagcca cattatttat atgcccttca tcaagtataa
     1321 gccaaattcc cacatatccg acaatttttc catttaactt agctactata taccttgcta
     1381 cttcattaga caactctttt ctaaaagact cttttgacca ataatcttca aaacaatttt
     1441 tttctacttc aaaaacttca tctatatctt ctgtagtcat ttcttctatt tttatatcat
     1501 ctactattag ttttgtatta tcttccatta tttagcctct tcattttttc atcatattgt
     1561 acttcagctt gagattttct gatatacata ggatttatcg tataacaagt atgtacatct
     1621 atatttttat tatatttatt taatgctaaa gagcataagc ttcctgcgtt agatacatta
     1681 ttagctggtg aaggtatttt tatatttttt atattctcta acttgtcttt atatttgtat
     1741 acagcttctc caactattat aaattcttca ttagttgata caatttcatc tactaaagca
     1801 tcaaaatcta caacatcaac accatctaat tctatcatct ctccattttc aaacttgtat
     1861 ttagctgaat aaacttgatt tttctgcgca tctaatatac aacatatctt tctatcacag
     1921 aaatttatgt tatttgctag agattccaaa gaatttactg ctattattgg tatattattt
     1981 acatgggcca tagcttttac tgtagccata cctattctaa gtcctgtaaa cgaacctgga
     2041 cctatacata cagctaataa atccatatct tttatactta aatcactcat actcaacata
     2101 ttttctatca ttggcataag cttttgtgaa tgagtttttt tgttgtttac tgtaaactca
     2161 catattaagt tatcatcttc aacaactgct acacttgctg ccatagaaga cgtatccata
     2221 cctagtattt tcattattta atcaactcct caactatctc tttataaaac tctccttttg
     2281 gagttaatat catttctctt ccttcatctt tatatctcaa ttctatattc aaatattctt
     2341 ttggaagtat atcttctatt agattagccc attctattat acagattcca tttgaattta
     2401 catattcatc ataaccaata tcatacattt catcactact acctattcta taaacatcaa
     2461 aatgatacaa aggtattttt ccttcatatt catttattat agtaaaagta ggacttgtta
     2521 tataatcttc tatccctaat gaatctgcca aactttgtgt cattgtagtt ttaccagctc
     2581 ctaaatctcc tactaaacag ataacactgc cttcttttaa aagtttacct aatttatatc
     2641 ctatttctct agttttattt tcattttcta aatatatttt tgccatcaat tcatctccat
     2701 tttgatataa tcgtcatgtg aatttatatc actgcttaat taattatagt ataagcctag
     2761 attattataa agtacttgtc tttatctttt tttatctttc actattgatg gctgtatcta
     2821 caccttatat acaatcatca atagcaattt ttacatactc ttttgcttta tggttttgct
     2881 tcaatgcttc tttacatatt ctctcagatt gctcttttac atacttaaga gacttatagt
     2941 tctcttttac tgcttcaata cacatttttt ctgtttgatt ttttatatac ataagcattt
     3001 ttccattttg tcttacaatc tctatacata tatcttctgt atacttatct atgtaactta
     3061 gtacatcttt aatattatat tttttattta aatatcttat agcatttata catatttctt
     3121 ctgtcttatt gttgacaaat tgtatacttc ttccatcttg tttgacagct tctatacata
     3181 ttttatcagt ttgtttattt acatattgta atgccatacc attttgtctt acggattcta
     3241 tacacatttt ttccgtttga ttttttacat attttaatgc tttataattt tgcttaatgg
     3301 cctctataca taattcttca ctctgttctc taacgtatgc aagacacctg ccatcttttt
     3361 taattgcttc taaacatatc cttcttgttt gagtctctaa agcctttagt atacttacaa
     3421 catctatatt ggaagaatga cttattgctt ttataataag cccttctgtt ttatttcgta
     3481 catacttaag tgcattacaa tcttttttta tagcttcaag acacaagtat tctgtttgtt
     3541 cttttacata ttcaagtgct ttataattat actttattgc ttctaaacat aattcttcgg
     3601 tttgattttt tacatactct aatgccttgt aattctgcct tattgctaaa atacacatac
     3661 catcagtttg attttctata ttttttaatt cacgataatc gtattttaca ctttccatat
     3721 tttcaatata ttcattagtg atatacttta aagaatctcc taattttgta tcataaatac
     3781 caatatgaat atctttccaa ttactattaa aataataccc tttatcatct aaaaataatt
     3841 tttttatttt tttagataaa tgcatgaata tacaccccta tcattattaa attagttatt
     3901 attaaaagtt tgattattaa tctttatagt tttttaataa taactttatt ttttgtttat
     3961 tataaattcg ctctgtatat tgtagatacc tttttcatat tttaatagtt aatcgtttta
     4021 attcttcctt tattttaaaa tttaaatttg tctaaatatt tcttatttta tatacatttt
     4081 ttgataaatt aataaagcca taatatattt aatatatcaa tcacaatata ggactgaagt
     4141 tataaataaa ttatggctat tcaatacatt taattttata ttttcttgtc taattatttc
     4201 tttattatat ttcccatttt tttataaagt ggcaatgtta ctaaagatat tataacagcc
     4261 ttcagcacat taaatggagc tatcatccaa attacaaaag tgaataaatc atgaacttga
     4321 ggatttatag ctgaacctaa acctattata gcatctagtc ccataagttg accataaagt
     4381 ggcaacatta caaaataatt tactaatgaa ccaattatag tcatagatat tgttcctaat
     4441 acaaaaccaa tcagtacacc ttttaaatct ccacttctct tatatgaata acaaagtatc
     4501 attacataag aaccaccaat taatatattc gcaaattctc ctattcctcc agttgttgtt
     4561 gctgttattg cttgtaacac attttttact acaactattc caaatccaac taatggtcct
     4621 agagacattc ctccgaatat tgctggaata tctgataagt caatttttaa gaaatctgga
     4681 aatatacctg gtattggtgc ttgaataaac attaataaat aagatattgc agataaaatt
     4741 gacattacta ctaaagttct tgttgaaatc atattgcctc tttttgctgt gttttgcata
     4801 attattttcc ccctaatcaa tatgtttata ttttttagga aaaacaaaaa actcgaagac
     4861 cctatcttcg agtttttatg gatatagttt aaacaaacta aaataaactt atttatcttc
     4921 cataaatatc ttctctcatc cagactttac tgtcggcttt ggaatctcac caaatcaacc
     4981 acctaagtag ttcgcgggct ataccgccgg tagggaattt caccctgccc cgaagatctt
     5041 ctttgatttt ttaattttcc ttacactctt atattagtta attttttaat aattgtcaag
     5101 ttattttttc tcatacacta ctttgccatt tattatagtg taaagtacat tactttgaat
     5161 ctctagtgga gaattatccc aaattactag gtctgcatct ttaccaattt ctatagagcc
     5221 tactctatct tctattccta aagccttagc tggatttatc gtaattgatt ctattgcttt
     5281 ttcttctttc ataccatgtt ttactgcaat acctgcacat attggaagat attgaactgg
     5341 tattactgga tggtctgtca ttatacatac atcaagacct gcatttgaaa gtattcctgc
     5401 tgtattaaat gtcaaatttc taagctcaaa tttagacctt tctgacaaag atggccctac
     5461 tattacagga aacccttctt ccactaattc atctacaata agatgacctt ctgtacaatg
     5521 gtctaatgta agctttaaat taaattcttt tgcaattctt attgctgtaa acatatcatc
     5581 tgctctatga gcatgtgcct taaaaggaat ttctcttctt aaaactggta ttaaactttc
     5641 catttttata tcatattcag gtttttcaca gtcttcatca tcatgactct catacatatc
     5701 aatatcctct aaatattctt ctgctttttt taggttttct ctaagtaaag cagctatagc
     5761 cattctagtt tgaggagatt tatcatcctg accataacag ctctttggat tttctccaaa
     5821 agcaatttta gatgcaactg gattttttat taccatttta tcaattcttt ttccacatgt
     5881 ctttatagct atacattgac cacccataac atttgcactt ccaggggttg tacaaacaga
     5941 agttattcca ccttcaaaag cttctttaaa agttctatcc attggattaa ttccatctat
     6001 aggatttaat tgtggtgtta ttgggtcagt ctcttcattt ccatctgctc cttcaaaacc
     6061 tataccatct tcccataaac ctaagtgagt atgagcatct ataaatccag gaaatacaag
     6121 tttattacta gcatctatta cctctacatc taaaggagct atcaaatctt ctccaatttc
     6181 tattattttt ccttcatcta taagtatatc tccatgaatt atcccatttg ttatggtatt
     6241 tattttacca ttttttataa aaatcattca ttttcctcct gatttttaag actatcgttc
     6301 ttattttaat atattttctt actatatact tttacaaatt tttaacattt atacactcga
     6361 gtaatctcat ttacaaatat agaatctaag tactataacc atacataaac ttatctattt
     6421 tttcattgat aaggcaactc tttttttgtt taagtcaatt ccaatgactt ttacatctac
     6481 tatatcacct actgtaacaa tactcatagg gtctttcaca aaactattac tcatttctga
     6541 tttatgaact aatccgtcat tttttattcc aatatctaca aatgcaccaa agtctacaac
     6601 atttcttatt gtacctttta aagtcatacc ctcttgtata tcttcaattt ttaagacatc
     6661 agttctaagt ataggtttta taccttcttc tcttgggtct cttcctggtt ttttgatttc
     6721 agctatgata tcttttaacg taacttgtcc tacttccagt ttttcactta attccctcaa
     6781 gccaatttct tttatttttt catctatttc acctatattc ttattcttta catcatctaa
     6841 agaatatcca attatctcaa tcattttttt acatatatca taactttcag gatgcacacc
     6901 tgtattatct agtggatttt taccctctaa gattctcata aatccagcac attgagtaaa
     6961 tgcttgaggt cctagtcttt ttactttttt caattgtgct cttgacgtaa aatctccatt
     7021 ttcttctcta tatgctatta tattctttgc tatagcttta cttattcctg caacatgttc
     7081 tagtagggag tatgatgcag tatttaaatc tactccaaca ctatttacag aatcttcaac
     7141 aactccatct agtacttctt ctaatctctt tttgtttaaa tcatgctgat attgaccaac
     7201 accaatactt ttagggtcta tttttacaag ctctgctaat gggtcttgta atcttctagc
     7261 tatagatata gctcctctta ttgatacatt tatatctgga tgttcctcat ttgcaagttc
     7321 agatgctgaa taaacagatg ctcctgcttc actaactatc acatactgca cttcgctatc
     7381 tatttctctt atcatttctg atacaaaggt ttctgattct ctagatgcag taccatttcc
     7441 aattgaaatt atatctatat cgtatttttc aatcaatcct tttaaaactt tctttgctcc
     7501 ctctacatca ttttgagggt ctgttggata tactgttgta taatctagta gtttaccatt
     7561 tttatctact acagctattt tacatccagt tctaaatgca gggtcaaatc ccataactac
     7621 tttgtcttta actggtggtt gaagcaataa acttttaaca ttctttccaa atacacttat
     7681 agctctttct tgagctattt ctgttaaatg atttcttatt tctctttcta ttgatgggaa
     7741 aattaatctt ttataagaat cttctattga acttacgatt tcttctttat ttttgaaatt
     7801 tttatcattt acatattcgt ttattatgta atttaaaact ttatcattat ttatttccaa
     7861 cttaaccttt aggaagcttt ctttttctcc tctgtttatt gccaaaactc tatgtggagc
     7921 catacttttt actgcttcgc tataatcata atacatatca taaaccgatt tctcatcagt
     7981 agcacttttg ctaactatca taccttctct taaagctaat tctcttatat actttctaat
     8041 cttagcatca tctgagacta gttctgcaat tatatctcta gcacctttta gagcatcttc
     8101 aatagaaagg acttctttct cttcatcaag ataattcttt gcctctattt ctatattatc
     8161 taaattattc tctagtattg acaatgctaa attttctaat cctttttctt ttgctattgt
     8221 tgctctagtt cttttctttt gtttatatgg agcatatata tcttctactt cttgtaatgt
     8281 ttttgctttt tctatatttt gtgttatttc atctgttaat ttcccttgtt catctattag
     8341 tctaactaca tcatcttttc tactttgaag atttctcaaa tacattaatt tttcataaaa
     8401 ttctcttagt gtaacatcac tcatttcgcc agtcatttct tttctatatc tagctataaa
     8461 tggtattgta tttccttcat caataagttt taatgtatta tttatttgtt catctctaag
     8521 attaaactct ttttttaaga tttgatttat atccaattta ttcactccta atgttagttt
     8581 ttcataagta taacaaaaca gaacgaaggc tgagggttat cgtcctcagc ctaagtgctg
     8641 taagtgcata ttaagcacta accttcttat acatatatct taattacagt gcatatttat
     8701 taaactattt tattcatttt taagtattca ttaataaaca agtctatatt tccattcatt
     8761 acactatcta catttccaaa ctcagcattt gttctatggt ccttaaccaa tttatatggt
     8821 tgaaaaacat atgacctaat ttgactaccc caagttattt gtgagtattt gccttgtata
     8881 tcttctattt tttccttttg ttctaactct tttaattcta tcaatttcgc cattaaaagt
     8941 ctcatagctc tgtccttatt tagatgctga gacctttcat tttggcactg aactactaca
     9001 ccagtaggga tatgtgttat tctaactgct gaatcagtcg tatttacatg ttgccctcca
     9061 gctccagaag ctctatatgt gtctatcttt aaatctgatg gattaatttc tacttctata
     9121 ttttcatcta actctggtgt tacatctata gatgcaaatg aagtatgtct ttttcctgat
     9181 gggtcaaatg gagatattct cactaatcta tgaactcctt tttcactctt taaataccca
     9241 taagcatttg ttccttctac aagaattgta gctgtcttta tccctgcttc agggtctgat
     9301 attatatcta aaagcttaac tttataaccc ttactttctg accatcttat atacattctc
     9361 aaaagcattt gagcccaatc ttgagcatct aagccaccag taccagcatt tatagataat
     9421 atagcattat ttttatcata ctctccattt agaagagtct ttattttcat ttcagataat
     9481 ttttcttcca ttgattctat ggacttttct atatctcttt ctacagaatc atcatcttct
     9541 tctaaaccta tttcaattaa tacttctata tcttctaata agctttttaa gctttcatat
     9601 tcttctaatg tctcttttaa agatttattt tcttgaagta ctctttgagc gacttcatta
     9661 tcattccaaa agtcttgttg attcatcttt tcttcattaa tttgtatatt tttcatcaat
     9721 gaagctatgt caaagagaaa ccctcaattc ttctaaattt gaagccatct gctccatact
     9781 atgtctatac tcttgtaact ttaacattta atcctccgag attatcttcc acaacagttt
     9841 ttatattttt taccacttcc acaagggcat aagtcatttc ttccaacctt ttcatctttt
     9901 ttaacagttt tacttgttgg aagtgttcca tctgatggag aagatagatt ttcaacatct
     9961 acaactgctt ttctttccac tggagtttct attgttatat taaataaata tcttacagta
    10021 tcttctttga tatgtttatt catttcatca aacatatcaa aaccttccat tttataagca
    10081 ataactgggt cttgctgacc tacagcacga agaccaatac cttggcgtaa ttggtccata
    10141 gcatctatat ggtctatcca atggttatca acagcttgaa gtaagattac tctctcaact
    10201 tctctcattc tctcatagcc aacttgttct tcctttgaag tatatatctt catagctatt
    10261 tcataaactt tttctgttat ttcaactgga ttaagttttt ctatttctgg tatttctatg
    10321 ctaccttttg gcaagaacaa attatacata tgttctttga atccttcttc atcaaatcct
    10381 ttatcttgtg tataaagagt aactgcttct tctattatag aatgagtcat tgattgtatt
    10441 tgttcttgta aatcttcacc ttctaataca cgttttctct ctgcatatat tatttctctt
    10501 tgtttattca taacatcatc atattgaagt acatgctttc ttattccaaa gtttttacct
    10561 tcgactttct tttgtgctcc ttcaatagac ttagataata ttctatgttc tatcggcatg
    10621 tcttcttcaa gacctatttt atctacaata ccagatattc tatcactacc aaacaatctc
    10681 ataaggtcat catcaagacc tatatagaat cttgaactcc ctgggtcacc ttgacgtcca
    10741 gcacgacctc tcaactggtt atctattctt cgagattcgt gtctttctgt accgattata
    10801 gcaagtccac ctgcttctac aacttgtttt tgttcttctt gtgtttgttg cttaaacttt
    10861 tcaaatagtt tttcatactc ttctctagct tcaaatagaa tttcattacc ttttacaggt
    10921 ataccttcta ttggagtatc tactctattt actatttctt ctttaaatcc atttcttctc
    10981 atttctctct tagttaaaaa gtctggattt ccacctagaa ctatatctgt accacgacca
    11041 gccatattag tagctattgt aacagctcca agtctaccag cttgtgctat tatttctgcc
    11101 tctttatcat gatgcttagc atttaatacc tcgtgcttta tgcccttttt ctttagtatt
    11161 tgtgataata attctgattt ttctattgat actgtaccta caagtattgg ttgattgact
    11221 ttgtgccttt caattatttc ttgtgctaca gcattaaatt tacctatttc acttttgtat
    11281 acacagtctg gtaagtcttc ccttatcata agcttattag taggtacttg gaaaacatcc
    11341 attttgtaga ttgctttaaa ctcttcttct tctgtttttg ctgtaccagt cataccagaa
    11401 agttttttgt acattctaaa gtaattttgg aatgttacag tagctagtgt tttagattct
    11461 ctttgtattt taagaccttc tttagcctct attgcttgat gtaaaccttc tgaatatctt
    11521 ctaccaaaca taagtctacc tgtgaactca tctactatta ctatttctcc atctttagca
    11581 acataatcaa catctttctt cattattaca tgtgctctta aagcttgatt tatatgatgg
    11641 tataattctg tatgagatat atctgttata ttatcaacat taaaatacac ttctgctttt
    11701 tgtatacccg atgctgttag agatacagcc ttatcttttt cttctaattc ataatcatct
    11761 ggttttaagg ttaaaacaaa tgtgtttgca tctgaatata agtgagttga tttgtctcca
    11821 ggtccagata tgataagtgg tgttctagcc tcatctatta gtatagagtc aacctcatcc
    11881 actatagcat aatttaatcc tctttgaact ctttgctctt tgtgtattac catattgtcc
    11941 tttagatagt caaatccata ttcattattt gtaccataag ttatatcaca atcatattgt
    12001 tcttttctaa cttttggatt ttgaccgtgt ataataacgc ctacactcat tcctaaaaac
    12061 tcatagattt ttgccatttg gtctctatca cgttttgcaa ggtaatcatt tactgttaca
    12121 acatgtacac cttttccagt aagcgcattc aaataaacag gagcagttgc aaccaaagtt
    12181 ttaccttcac ctgttttcat ttctgctatt cttccttgat gtagtactat acctccaatc
    12241 atttgaactc tgtagtgtct aagtcctaac actctcttag aaacttctct aactactgca
    12301 aaagcttctg gaagtatatc atctatactt tcaccatttg ctaatctttc tttaaatata
    12361 tttgtcatat ttttaagttc cgagtctgcc atagattcaa atttaggttc taatgaatct
    12421 attatatcaa cagttttatt gaactttttt aattcttttt tatctgccat gttgaataaa
    12481 ttatccataa agctcatatt ttacatctct cctcgttctt tattgtacta ttattcattt
    12541 taccataaaa actatacttt taatattaat ttaaaaagct atcgataatt tatttatcga
    12601 tagctaatat gattaaatct aaattgcttt attcatgttc tattatacca tatccaccat
    12661 tattacgctt ataaactact gctatttcat ctgaatctat gttcttgaac atataaaaat
    12721 catgtcctat taattccatc tgtaatacag cttcttcttc actcattggc ttaacactaa
    12781 atttcttacg tctttgaatt actaattctt gattttcatc cccataatcc tcttgttcat
    12841 cagctagatt tatctctgca tcttggaatc ttatactctt gttgtcttta tgtttatctt
    12901 gtaatctctt tttatatttt tttaattgtt tacttaattt atcatataca acatctatag
    12961 ctgtgtataa attttcctgc atatcttctg ctctaattat aggaccattt acagtagcta
    13021 ttgttacttc tattttttgt cttgcttttc tcgcactcac tgtaactttt acatcagtgt
    13081 ctggatgaat gtaattatct aacttactta gtttagtttc gattttattt tttattgcat
    13141 cagttaattc gatttgtttt ccagatataa ttatgttcat aaatacatct cccttcaatt
    13201 atgccagtat aagaaaattt atatttaata atatattcta cacgaactct cataatcctc
    13261 tttttattat ttacttttat gattttttct attcaattga tagaattatt tatcattttt
    13321 agttacattg atacttatgt ggattaatat tattacaaac acctgtggat aatgtggata
    13381 aaactgttaa taactcttgt ttttgtttat ttaaagctat ttttactcct atttctatcc
    13441 acaaaaatct ccattaattg tttattaata tgtgaattca tgtcataaga tggtaatttt
    13501 aggaaattat gtgtattata tgccaatata cctttataat tttttaataa ctataaaccc
    13561 tctaaaacct ccataatcta tcttttcttt cattatttat atttaaaact aattaaaagt
    13621 tataaagcat actcaacaat aatttcatat ttaataccta ttaacattca tatacccaaa
    13681 ttataattaa cttactctct ttatttaaac taagtatttt atattcatat actatcttga
    13741 aattatattt atgagttgtt taagcttttg tctatctata tcttcaaaag gactgttatt
    13801 tattctaatc gctgtaccaa aatgaaaatt acttgcctta gttaattcct ttatcttttc
    13861 tatattatta aaattaagac ctccacctaa aagaattttt atgtgattag aactaagcat
    13921 catattcttt atcatttgta tgttatcaac tatacttcct ttccctcctg acgttaatat
    13981 attcgtaatt ttatcataat ccttcaaaat tttaacacta tctattgtat ttgattcatc
    14041 tattgctcta tgaaatgtaa catcaagatt atcacaacat ttcaataaaa catttagatt
    14101 tttctcatct atattattgt ttttatccaa tacaccaaat acaactccat tagcaccaat
    14161 ctctttaacc acacttatat ctttttgcat tatacttata tcttctttac tgtatacaaa
    14221 acttttcgca tgatgtctta tcattacgtt cacaggtatc tttacactgt taacaacact
    14281 ttctataaga ccaaaacttg gtgtcaatcc accttcagtt aaagcactaa ctaactctat
    14341 tctatctgca ccacaatctt ctataatttt agcgtcttct actgacattc ctattatttc
    14401 taacataact actcctttgc taattcatat attgcatttg caaatatctt tgcatttaat
    14461 attatatctt ctatatccat gtattcatca ggattatgag ctattccttt ttgtcctgga
    14521 aatgaaggtc caaacggaac tatatttggc attacttttg catacgtacc tccattagtt
    14581 gttacgggag ttccatctaa tgatgtaacc ctttcataag ctagctgaag ctttttaaca
    14641 agattactct ctttatcaaa atatacagga ttaaaattag aaattacctc tatatcgata
    14701 ctatttggaa aactagcttt tatttcatcc aatacatctt tcaatttata actaaaatcg
    14761 tattttacct ttatatctaa tatagtatca ctattttcta tattcagttc tttatatgta
    14821 acaagcacat tttcagacat attttctata aaacctaggt atacatcttc ttcataattg
    14881 attttacttt ttattctaat ctttgccatg cctttttcac catatacaac aggatactta
    14941 caatctggtg taaatcccat aataggtgct ttttcttttt ctaaataata tggtatatct
    15001 tcaaatccac tttcttcatt tgtaccaaat attattctaa ctctcttatc taattttaag
    15061 tttaattctt taatagcata taatccatat aaggcagaca ttataggtcc tttattgtct
    15121 agtactcctc gtccataaat tcttccatta gtttcttctc ccttataagg ctgatgcttc
    15181 caaccatctc cttcatgtac aacatctaca tgtccaatta cacaaacata atcatcacta
    15241 tctccatgct ctgcataacc aatataatta tccaagtttc taaccttaaa tcccaacttt
    15301 tctgatattt ctaatgcttt attaagtgcc tcccctactt tttcgccaaa agggaagcca
    15361 ttttctgact cccctttgac actaggtatt tttacaatat ctataatatc acttaataaa
    15421 tcttctttta actcatctat tttattactt atgatattca taaaaatctc cttattataa
    15481 aactctattt taattatgta accactactt tactcactta cttctattgg tgcttttatt
    15541 ctagcctcat atgcttccat ccactcattt gatataacct tatgtatagt ctttccatct
    15601 actctatttg aaatatatac agtaggagat tcttctttag ttactactga aaaagtaaaa
    15661 tcagctatat ttgtagctac tccccattca cctttattat tcatggcaac taatgataaa
    15721 tcaccagctt ttcctcttct tttgataagc tgtttatcaa gttcatttac tgcaatatca
    15781 catgcttctt gtggatgctt cccttctttc ataagtctaa ctatctcata agatatacat
    15841 cccttcatta aatcttctcc caaaccagta gctgtagctc ctcctatact actatcaaca
    15901 tagaatcctg aaccagatag tgcagaatct cctattctac ctcttttctt cataaacaat
    15961 ccacttgttg atgtagctac acacattttt ccagttttgt ctaaagatat cataccaaca
    16021 gtatcatgac cagcatatgg gcttaatcct ttgtctaaag tctctttttt tctcttttta
    16081 taatgtaact tcgctctatc agtcaacata ttttttcttt caaatccatt tttatgagca
    16141 taatcttcag cacctattcc aactaaaaaa ttatttacct tctcataact taacttccta
    16201 gctatgctga caggattaga ataatctctt ataccagcaa ctgcgccaat atctaatgtg
    16261 ttaccatcca taaatgcagc gtcaagttct acctcacaat tttcatttgg aagtccacca
    16321 tatccaaccg atttatagaa tggatagtct tcaacccttc ttacagcaag ctctacagca
    16381 tcacctgctt ccatgccttt ttctaaatgt tctgatgcct cagttattcc ttctagggcc
    16441 attctccatg tcgctatcat tccccacatg ttaaaattcc cctccttaaa ttatatagat
    16501 tttactaatg tatgcttatt ataaatagta taatatttaa acttgctaat aattaatact
    16561 cttattaata acaaaattct tctttcatca aagttatatc ttctttattt gctattgctt
    16621 ttgccaattt acatatgtta tttaacgaat cattaagacc aattccacct ttttttggag
    16681 tcttaactat acatatgtca tttttatatt cgtttatcac atctatattt tcttctgaca
    16741 tagctgcaaa agctggtata tctgttttat catttatatt tttagacagt attgctgaca
    16801 tcttagaaaa gttttcgtaa ttaaagcttg gtccacagat tactacatca gggtttaatt
    16861 tttttaccat agctatcatc ttactcgtta cttcttcttg attatttaca aaaaattcat
    16921 ccccacaaaa taaggtagct attaccttag attcatttaa aaattgctcc atcattacac
    16981 ctggtcctaa tgggcttgat tttccagcag gaggtatatt acttttctct tttcctccag
    17041 ctccagcttg tatttggtcc aatatcataa ctattttctt cataatcttc ctcctataat
    17101 actaaatcat caaaagatat atcatcatct tcctcttcaa cagtttcatt ttcttctctc
    17161 aagtattgct tatctagtat ttttataaat ggtatataca taaatattcc tagtataaga
    17221 agtactaatt gtaagactgc accttgccat cctgttgtta aaaaaccaga gaatataatt
    17281 ggagttgtcc acggtaactg aactccattt gtaagaggca ctaatcctgc tgacatacag
    17341 aagtaagcta ttattatatt tattgttgga actattgcaa atggtatcaa tagtattggg
    17401 ttaagcatta ttggtagacc aaatatcaat ggctcattta taccaaatat tcctggtaaa
    17461 attgatagtt ttcccattga ctttatacgt ttactcttac atattaataa catagctatg
    17521 actaatgata gtgtactacc tgctccacca aatgtcgcaa acatatcttg gaattgacct
    17581 gttattatgt ttggtatttt tgaaccagct tgaaaagcag ctaagttttc agctgataga
    17641 atttttaata ttggattata cacagcccca acaacactac ttccatttat tccaaagaac
    17701 cagaataaat gtaagaatat ataagcgatt gccatagcac ctaaactatt tcctaatttt
    17761 aataatggca tttgtaaaaa cttaaatata aattcatgta tacttccata tggtgtagct
    17821 tcaaaagcta tcttaactaa aaagaaaact accataacta ttgcacttgg tattaatgct
    17881 gcaaatgatt ttgatacagt tggaggaact ccatctggca ttttcaatgt ccatcctttt
    17941 tttacaactg cagcaaataa ttttacagag gttattgctg ttatcatacc tacaaacatt
    18001 cccttagaac ccatccaacc tagtggtata cctgttacca tgtaaactga ttctgttcct
    18061 tctggtgtat atggtattac aaatggtgtt attataaaaa aggcaactat tgcaactgct
    18121 gctgatgcta ttgggtctac atctatttgc tttgcataag aatatccaat ccctaaaact
    18181 gcaagtagag tcattacttc aaatgttgct acagttggtt gttgcaattt tacagcccaa
    18241 tctggaccaa aaatttgacc tacaaattca ttccatcctt ttataggaaa atttgctact
    18301 aataaaaata atgaacctat tattaataat ggtgatgata ctaaaaaacc atctctaatt
    18361 gcaattaata atttattttt acctattttt tcagccaatg gcataagtat tctttccaat
    18421 ctattcatct atccaccctc atttcctatt attttttact ataattctac gcttgtttag
    18481 attttattag ctttatagca agttttaata ctttttcccc attcatagtt ccataatcaa
    18541 cttcattgat tatatctata agttttcctt gagcttcaaa ctttggtttt aactccttta
    18601 gcatgtattt aacttgtggt cctaataata tacaatctgg atttttttct tcaactattt
    18661 tgtcaatttc tgctatcgga aatgcctcta cttctattgg taaattgtgt tcatctgcta
    18721 ccttctgcat tttatctgca agtaaacttg ttgacatacc aaagctacaa aataaatata
    18781 cttttctttt cattttttaa atccccctta atttttttat tttaattttt gaatttcttt
    18841 tcttaacata atcatttctt ttataaggtt tctctcactc atacaagtca ttaaatggtc
    18901 ttgagagtga atcaataaaa tagatatatc tgatttatct cccccagctt ccttttgtat
    18961 aagttcagtt tgtactttat gtgcttttaa aatttcttca tttgcatttt caattaattc
    19021 atctgctttt ttaaaatcgt tttcctttgc atagtcaagt gcttcataaa gcatagattt
    19081 tgcatttcca gcatgaatta taatctttaa aactatttct tccatttttt cctcctaagt
    19141 tacttctcta cttttagatt atttatacct tacaaacata taatatctta taaaaacaaa
    19201 aaaacatgca caactttttc catcacatgg aaaattttgt gcttatccat agttcactat
    19261 gaattttata taagaatatc ttttaccttt tcaatgtcac atttacttag aatatctagc
    19321 atattttcat cctctgttaa attagatatt tctttaaata attctctaaa tacttttcta
    19381 tcatcatctt ttttatatgc taacaaaagt ataatcttta tatcctttcc atttctaatt
    19441 tttattggtg tttttaacct tgcaaaacat aaaacagatt gttttatata tttcatgtct
    19501 ccatgaggga ataatattcc ttttccaaca ttggttggat atgacttttc tcttgttata
    19561 atagattgta aatactcttt ttctgcataa tgtttttcaa ttaataaatt agttaaatat
    19621 gttatagctt cataaatatc atcatgttct aactctctta ttaaataatc agacatcatt
    19681 aatctgttca taagtttata tgaagtgtct ttttctttta ttttgtttgt gaactcttta
    19741 agtttattct catcctcatc agtaaataat ggtgttgtta taaaaattgg tttttggata
    19801 ttttcatact cttgaaatgc aattataaaa tctatagtct tattaaagtt atctcttttt
    19861 aaatcttcct ctcttaatgt ttcaataatt tttatgttat caaatctctt ttctatcttt
    19921 acttttaata gcatacttac tccaaaacta aatggacata caatacttgc ctttttattt
    19981 gattcccttt tgctgtattt tctttctaga gaggtttgaa agtgaagtgt aagataacca
    20041 atttcatctt ccgttatttg ttttcctttt aattcaattt ctattgaact tgatattact
    20101 gtaaataaaa aacaaaattt agtttttatg ttatctaaaa gtggattttc taagtaaaca
    20161 ttgcttttca tttgatgcat tgtaacattc aaatgacata ttaattgttc ataaagtaat
    20221 atatcattat taaaatttat tcctgattct tctgatacta aatttataat tttttttgta
    20281 taacttgcta attcttcatt tagatataaa gatttcttaa tattattact atttatttgt
    20341 ttgttcatac ccaatattaa agattctata aacaattttt cttcataatt taatactatg
    20401 gataaattag atttcaattc atcttctata tccaatataa gcatttttag cttaagctct
    20461 ttcttagtat ttgatacttt taaagaattt cccagtctta ttcgtattat atttataagc
    20521 aaaaatagca tcaattgctt gaatgatata tctgtaagtc ttatatccat atcctcctct
    20581 atagtaagca ttatgttttt tacaattatt atatctacta tatggaagta ctctatttca
    20641 tctatgttta atctgtattt atctatatat ttaaataaga atgatactaa cattattctt
    20701 atgtctttct catccccttc aatatatata ccactgcctt ttttagtcga gatatttaaa
    20761 ttataattat ttaatgctac tgataattta cttatttctt ctctgacaat atttttattt
    20821 atgtacaact tattagataa atcatttaaa gtaacttttt tattattaca aagtagtaaa
    20881 tttaatatct caacttgctt atgaagctca ctatttactc ttttaacttc acctcgcaac
    20941 ttctgattta ccagtatttt gtctgttcca tctccactaa atttaatccc aatattaggt
    21001 tttcttttga tataagcatt acaaaaagta gataaccatt catcaatata tttgcaatca
    21061 tttcttattg tcctttctga acactcaaaa tcattggcaa tcttattaac atttataaac
    21121 tcttctttta atagcaatgt tgttaataaa ttttcttgtc ttttattcaa acatatcacc
    21181 tctttatgac tcaacctaaa tttattatat ataaaaaaga gctatacata aaggtatgct
    21241 agctctcttt taatatgata tttagctgac ctggtttttg accccacact cgcctactgg
    21301 agggttcgct tgggttcgcc gcactactac tctctctcga ttttattatc ggtaggtctt
    21361 acttctaagc cgcaccatga tcattaaccc aatttttagt gttaacttac gtggatccct
    21421 ttgcctgcga ctggcttgga ctttcaacca cagacctaaa tatcacttat tcattttaat
    21481 attttaaact aaaattgtca atatataaga ttattccata atataattat catttgcttt
    21541 tgtaagtaga gtcaatacaa aaaccttatt agcacctgat aactttaaaa gtttagaaat
    21601 ttcgtttgtt gttagaccag tagtaaatat atcatctatt aaaagtatat ttttattatt
    21661 tattaattta acattttctt taacaacaaa tacatttttt aattcttctt ttcgttcttt
    21721 cttattcaat ttatatagca ttcttgtata tttttttcta cttatgcaat ctaataatgg
    21781 aatatttact attttactta aattaaatgc tattttttgt gcctgattaa aacctctttt
    21841 atttagcctt tttttatgaa gtggaacaaa taatatataa tcaaatttta tgttttctaa
    21901 atataatttt tctttcatta tttgagctat atacttagcc ataaaggttt tttggttata
    21961 cttcagccct aacaccatct tttttgtcac atcattgtat tcaatacaag atattgattt
    22021 atcaaaataa aagtctttat taaaacaata actacactct tcaataaatt ctttttcaat
    22081 tgaatgacgt attattggtt ttccacactt catgcaacca tcttgtataa aattcatttc
    22141 tttaaaacaa cttttacata ttgaataagt atttgttttc tttatggatt tatcacaaat
    22201 tatgcaactt atattctcag gatatataaa atccaaacat ttattaatca tatttttaaa
    22261 attcttaata aaattatcat tgcatatttt catatttatt ctatcaacaa cccttcttgt
    22321 ttaaatttat ttagcttata tcctaaattt gaataccttt gattaactct attatttttt
    22381 atcatatatt caagatattt cacatctcca actaatacaa ctaatttttt tgctcttgtt
    22441 actgctgtat ataataaatt acggctaagt aacataggag gagcccacgc tattggcaat
    22501 actacaactg gaaattcact tccctggctt ttatgtattg ttgtacaaaa actatggtca
    22561 atttcatcta attcatcata taggtatgat acaattttag tctggtcaaa taatatataa
    22621 atagttttct tatctttatc tatgtgataa acatatccta tatctccatt atagatacct
    22681 tctccacttt ctttttggtc ttctgtttcc catttttttg tataattatt ttttacttgc
    22741 attaccttat cgccaactct aaataatctt ttagaaaaac tttcttcaac tttaaatttt
    22801 tcttttttgt taagatactt ctgtaactct atattgagat tagtaactcc caattcacct
    22861 tttctcattg aagataaaac ttgtatatct tttaatttat caactttata aaatttaggt
    22921 aatctttcat taaccaatcc tattatttca tttaaaattt cttcattagt tgattttctt
    22981 ataaaaaaga aatctttacc ttttgtattt agataaagtg gttctccatt gtttatctta
    23041 tgggcattta caattatcat actttcttgc gcttgtctaa atatttcatt taatttaact
    23101 acatttataa tattagaatt aatcatatcc ttaagtacat ttccagcacc tacagaaggt
    23161 aattggtcac tatctccaac taaaattact cttgttccta actttatagc tctaagtaaa
    23221 ttgtacatta agataatgtc taccattgat acctcatcaa caattattac atctgaattt
    23281 attgggtctt cttcatcctt aaaaaaagtt aaatcatcat ctgttgaaaa tcccatttct
    23341 agcaacctat gtatagtttt tgcttcttta ttagaagttt cactcattct ctttgctgct
    23401 cttcccgttg gagctgccag agtcacactt ttaccattat tttcaaatat atctatgata
    23461 gcattaatgg tagttgtttt accagttcct ggtccaccag ttataattaa tactccacta
    23521 ttaacagatt ctttgactgc taaaatttgt ttttctgcta acttaatttt cttatcttct
    23581 tctaaaacat taatctcgtt atctatatct atttttaagt cttcaaattc atactgtgat
    23641 aattttacta tttgactaca tactccattt tcagctaaat agtatggcat aaggtatata
    23701 agattttcat tgtttacttt ctctatatgt attttttgat tataaactag catcattaca
    23761 cactctttta ttatttctcc atttagctcc aataattttt ccgaatcttg tattagaaca
    23821 tgctctggta aatatgtatg cccattactt aaagatttat ttaaagtgta taagattccc
    23881 tgacaaattc tatctttaga gtttttatct ataccaattt tacttgctat actatcagct
    23941 actttaaatc caattcctct tatatcttca gcaagttggt atgggttttt atttataact
    24001 tctattgcac tgcttttata ttttttatat atttttaggc agtaatttgg tgttattcca
    24061 aaaggagata gctgtattat tatatttctc aattctctgt cttcttcata actttttact
    24121 atttgtttta cttttttact ccctatacct tctacttctt gtagtttttc tggtgaattt
    24181 tgtatcacct ctaacgtatc tacaccaaac ttatcaatta ttctttttgc cattttttca
    24241 ccaataccat gtatcattcc agaagataag taaacataaa ttccttctaa ggatgatggt
    24301 gttactggca taaaactatt cacttcaaat tgagtcccat aaatcttatg atttacccac
    24361 tttccttcaa cttctatgct ttctccaatt gccaaagtag gcatacatcc tacaaccaca
    24421 atttcatcat tttcatttac caggtgagct attgtataac cattatcttc gtttttaaag
    24481 actatttcac ttatcattcc ttctaatttt tccattttta tctcctaaaa atactaataa
    24541 tcgctccatt taaatgttag tataatttta tatattaaaa ctatgataat cgtagaaaag
    24601 ttttatattt ttatacttaa cgtaatttcc aactattata cattttatca gaaacaaaaa
    24661 aaatctgcat ccataaaata tggtaccatg aaatacaggt accatattta aatagctatt
    24721 ttattttaat tgctctatat ttagagtata aattaaattc cataagtata ttacagtact
    24781 aacttatttc agatttattc tccttgtacc cctcagtttt agtaattctt ctataaagca
    24841 tacatagtaa aattaaaatt cccatgtaac ctgtatatgg atatagtgta cctactaact
    24901 tatcaaatgg cagtaaacct cctatacaag ctaatatact taccactatt gttataattt
    24961 taaacttagg gtggtcatct tctacaaatc ggtttgttac tgaccataac aatggtacag
    25021 ctgtagtgta tattcctagt aaaagtacta ctgagaataa aatacctatt attggagata
    25081 tcttatcagc taagtaaagt gctggtatct cttttgtgta tatatttcct atgtctgaaa
    25141 gtaatgccaa attcatcatt atagctgctg ccataagtgc tactccacct actataccac
    25201 cccatacagc atctttctta tttactgcag aagctcccat tcctgtcaag aatgttatta
    25261 ctattattac attgtaacaa gtataaagta ctcccgagaa atatccattt gatgtagcag
    25321 ttttaatatt taaactatca accatatttc cagcatttga taaagcatca atattgctaa
    25381 ataaactaat tgctcctact aaaagtgtaa atattattat tataggtcct atattaccta
    25441 gtattttaga aagccttgtc aaacctaatg aaacagttat tagggaaacg attgccatac
    25501 caatcctacc tacatatgga tttaatccat agtactctga taatgtagct cctgctcctg
    25561 caatcattat tactaatgtt ccaaataaaa acaatggacc aaaccattca aaaaatgttc
    25621 caagatattt tccacaatat acttgataaa tttttattgg ctccttaagt ttcaattctc
    25681 ttcccttatc tataacttct gctccaaacc atgaaaatag taccatagag attattccac
    25741 ctataatacc cgcatatcca taaaacgtaa aaaattgcag tatttcttga ccagttgcaa
    25801 atccagaacc tattacagta gcaacataag ctccagcaaa acttataacc gttttaatat
    25861 ttactttgcc tgacatacac tactcccccc caattaaaat tatttttaaa gtgtaagttt
    25921 attaagatta tattagactc attattgtga tatttcaata tatttttaat tatttaacat
    25981 tagttaacac attcttaacc atcctttaac atatatccca taatatgtat aaatttagta
    26041 gagtattata taaaatgtat tgaccagatt aaatactgtt tattcattat atgatgataa
    26101 attaacattt attaaaaata tttaactcat aataaaaaag ctgttagaaa gtaatcgtaa
    26161 aattacattc taacagcttt ttagttaaaa attattcacc taaagcatct tttcttaact
    26221 gctcaacctt gtcagttctt tcccaaggta aatctatatc agttctacca aagtgaccat
    26281 aagcagcaac ctttttataa agaggctttc ttaaatctaa gtctcttatt atcgctcccg
    26341 gtcttaaatc aaagtgttta tttactaatt caacaagttt ttcttctgaa accttgcctg
    26401 tcccaaaagt atctataaat atagataatg gtctagctat acctatagca tatgcaagtt
    26461 ctatctcaca tttgtctgca agaccagccg caacaatatt ttttgcaaca tatctagctg
    26521 cataagcagc agatctatca acttttgtag ggtcttttcc tgagaaagct cctccaccat
    26581 gtctagaata tccaccataa gtatctatta ttatttttct tcctgtaaga cctgtatctc
    26641 cttgtggtcc tcctataacg aatcttcctg ttggatttat ataaatttta gtctcttcat
    26701 caagtaattc tgctggtata acttccttaa taacatgatt aattaaatct tctcttattt
    26761 tatcatttga tactgtttca caatgttgag ctgatataag aactgtatgt actcttacag
    26821 ctttacttcc ttcatattca acagtaactt gagtttttcc atctggtctt aaataatcaa
    26881 ctaaacctgt ttttctaact tctgtaagtc ttcttgataa tttatgtgcc aaagatatcg
    26941 gaagtggcat taattctgga gtctcattac atgcaaatcc aaacattata ccttggtctc
    27001 cagctccaac tttttctatt tcttcttcta tctcttctcc agtcttactt tctagacctt
    27061 catcaactcc catagctata tctccagatt gttcatcaat agaagttata actgcgcaag
    27121 tatcacaatc aaatccatat tttgctcttg tatatccaat ttctcttact gtttctctta
    27181 ctaatttagg aatgtctaca taagcagatg tacttatttc tcctgctact aaaactaaac
    27241 cagtagtaac tgtagtttca caagctactc tagattgtgg gtctttttct aataatgcat
    27301 ctaatattga atctgatatt tggtcacata tcttatcagg atgtccttct gtaactgatt
    27361 ctgacgtaaa taaatgtctt gccattttta ttccccctta tttattctta atttttatta
    27421 taggttttac cttattttta attttagaaa tttactcaaa ataaaaaacc tctcctttat
    27481 aagaagaggt taataatcgt aattacatac taattaactg acctcatctt tcaagacttt
    27541 tgtcttgtag gaattagcac cttttcccat ttgggtggtt gctgggcttc acagggccta
    27601 tccctcagcc tctcttgata aggtaatcaa gatttaagtt ttgttttact ttactagctt
    27661 actatttttt taaaattttg tcaatatttt tagtcatatt ttttctattc ctttttttgt
    27721 aagacaaata aaattttcta tctatctgaa atttaaagtt aggtcttttt aatgcttttt
    27781 attcatatat ttttttgaag ggggtgttcc tattgtattt agctaaagta aactacagcg
    27841 atgatgatgt tatacagatg ctaagttcag ttctaaaaac tataattaat gaaaatacga
    27901 ttgttgtttg cataggaaca gacagggcta taggtgatac tttaggccct ttagttggaa
    27961 caatacttaa gaacagtaac tttaaatacc ccgtttacgg tacactagat aaccctattc
    28021 acgccttaaa tatatatgag tctttagata ctataaaaaa tacacatata caaggtaact
    28081 tcttggctat agatgcttgc ttggggtcgc agagtaacat aggtaatata cagattagag
    28141 aaggacctat cctgcccgga aaaggtgttg gcaaaaaact acctcaaata ggaaattatt
    28201 ctatagtagg aattgtagat aaaattgatg aaaataataa actttcattt aataatatac
    28261 gccttagctt tatattagat ttagctgaaa caatagcctt ggctttatta gtatccacct
    28321 aatctaaata taaactaaat gataaaattc tttatataaa aaggtagata ttttagagta
    28381 tctgattaaa tactccaaat tatctacctt tttactttta tatttcaagt ttttattttt
    28441 ctactaaagc aaacattaat tcacctttac agacaagttc ttcacctaca tatgccttag
    28501 catctgcctt agctatattt tttctaagag ttgtcatttc aacttccata gttaatgtat
    28561 ctcctggtct tacttctctc ttgattctaa ctttatctat acctgcaaat acacctagtt
    28621 ttcctttgtt ttcctctaca gacatcattg ccactccaca tacttgtgct agagcttcta
    28681 ctattagtac tcctggcatc aaaggatatt ctggaaagtg accttggaaa aatggctcat
    28741 taactgttac attctttata ccaacagctc ttttcccaac ttctaactct gtaatcttgt
    28801 ctactaataa aaatggatat ctatgtggta ttaatttttt tatttcatct atatttaaca
    28861 ttctaaactc tcctaattat tttcttttga aagacccacc agtttcaagt aagtctattg
    28921 aacctaaagc tattcctgta ccttttgcaa cacaagataa tggttcatct gctattgtaa
    28981 cagttattcc tgtcctctgt tctattattt tatctaatcc atataataat gaacctcctc
    29041 cagtcattat tataccgaca tctcctatat ctgctgctaa ctcaggaggc gttttttcca
    29101 aaacagcatg agtagcaact actatttgct caacacattc tctaagtgct tccaacattt
    29161 ctgttgaatt tatagttata gaactaggaa gtcctgtaat taaatttctt cctgttatct
    29221 tcatatattt ttcttcttct ctcttaaatg cagaacctat ttcaaacttg attttctctg
    29281 ctgttctttc tcctataaga agattgtgtt gttttttcat atatttaact atagcatcat
    29341 caaatctatc tccaccaacc tttatagatt cacttacaac agctcctcct agagatatta
    29401 cagctatatc tacagtacct ccaccaatat ctataatcat attaccatta ggctgtgata
    29461 tgtctacacc agctcctatg gcagctgcta ttggttcttc tatgatatat acttctctag
    29521 ctccagcttg tctcgtagca tcttctactg ctcttttttc cacttctgtg acacctgttg
    29581 gtacacatac cataattctt ggcataaaaa atcttccaaa tcccttttta tcaactatct
    29641 tatctataaa agattttaac atcttctcag ttacatcata atctgaaatt acaccatctc
    29701 ttaaaggtct tatagcgact atattacctg gtgttctccc tatcatcttt ttagcttctt
    29761 ctcccacagc taaaacagaa tctgttcttt tgtctatcgc cacaacagat ggttcttcta
    29821 aaacgattcc cttaccacta acataaacaa gcacatttgc agtacctaaa tctatgccta
    29881 tatcagctcc agccattttt tcaactcctt tatttataat attagattat attataagcc
    29941 tattttaata ctcctataat aaagcatata caaaaatctc gctttaatca agagaaaata
    30001 aatcttttga gagtattatt attttgctat ttataattac ttaatatatt tctttttata
    30061 atttaattca ataaaaaaag taggttttta acctacattt ttttatgttc cttctcatat
    30121 tttaatttcg ttgctaatcc ccctctaata tgtctctctg atttattttt gtccagcaca
    30181 tttttaactt ctttggcaag ggatggattt atttccttca atctttctgt aacatcctta
    30241 tgaattgtac ttttacttac tccaaatgtc ttggcggttt gtcttactgt agtgtttttt
    30301 tcaagtatat attttgctac cactatggcc ctttcctcta tatgagatct caaagattac
    30361 ccccctcaca taatagcttt aatgatatat atgtaaaaac accaaaaaat ataacaagct
    30421 taaccaaata tgattaaact tgttatacta ttacttaatt agactcattg ggtctataga
    30481 tttttctcca ctatacgcct ctaaatgcac atgtatacct tcttcacttt ctatttgaga
    30541 agtactacct acagtaccta gagattgtcc ttctgttacc ttctgttctt tcttaacact
    30601 aacattttta tctagatttg aatatactac tacaatgtta ttgtcacttt ttattttgac
    30661 tgattgtcca tattcttcat catcaaatac atctactact attccgttta atagagattt
    30721 tacttctttg cctttagtac aactaatatc tacaccttta tgagtttccc aaacatcaag
    30781 tgttttagaa taactaggct ctttttctga atatcctctt attactttat ctccaatgta
    30841 atttaatttt gttgtactcg atttattttc ctcttttgac ttagcttttt ctaagttttg
    30901 ttttgaatcc gttgcagtag ggatagcatc tttcttgtct ttttcaatta aatgtatttc
    30961 ctcttcacta tctttattag cattttccat aattccttta ttagaagcca atttatctac
    31021 attattattt gtaaaccaaa ctccacctac tgctaataaa caaacacata caaacaaaga
    31081 taaataaaaa ccatcttttt ctaacagctt tttcttcatt ttgcacctcc aaatatattt
    31141 ctacatttgg attattgacg tgtttttggc attttataca attattagta tatatctttt
    31201 atttttgtat ctgtgtagta atgtgataat atatcataat atttatagcc ctcttctgcc
    31261 attccttctg ctccccactg gctcatacca acaccatgac catatccttt taccacgaaa
    31321 tctatgtatc cttctccaaa ttttatgtca aagtttgcag agttcaattt aaaaacagtt
    31381 ctaatgtctt ttccagtcaa ttctttattt cctaatttta ttttttctac agttccggca
    31441 tcacttcttt tagttattga aacttgttta cttaaattat tcacatctat cacaatagtg
    31501 ctatatgccc ttctaagact tttaacaaaa tctgtgttac ttattttaag tgttgacgca
    31561 aatttagggg aatacttatc ataaggactt tccacagact ttaaataagg atattttgca
    31621 gaaaatactt cttcactatt ttctgttttt cctgaagatg tagaaaaata aagaggaagt
    31681 atcgccttat cattataagt tattatttga cctttagttc ctctaactgc ttcttggatt
    31741 ttggaatatt tattcttaat ccattcttca ccatttaatt ttttaagagt atcataactc
    31801 ttatattctt gacaatgttt ataatcagta catactactg catttttatg tttactagat
    31861 ttaccatgtt cctgtttata tactacatat gttctagctg ctacagattg agcttttaaa
    31921 gcttctatat caaactctga agacatctca ccagctagta ctccacataa atagttctcc
    31981 atgtccatct tttcggtttt ccctgttata tgattatata cattgataat tggtgctttt
    32041 ttatttacag tttcatagtt cactgtattt ttttcttctt tattaccttt gtcttcaata
    32101 tcactttttt taatagtttt gtttatagat actgggctct ctgctttttc agttaattct
    32161 acatttttat aagaaactaa agttataagt gatggaacta acacagagca agtcacaaat
    32221 cctaacaaaa caaccaatgg gttcttcata tactctcctc cttagctttt aaaaccttgt
    32281 atttaagtat atgaacaacc ctattaattt ataactttct tctataaacc aattattaat
    32341 cttctactat ttcgatttga ccacctagag ctgttatctt cttatctata tcaacataac
    32401 ctctttggat atggtatatt tctcctattt gagtttctcc ttctgctata agaccgcata
    32461 aaataagggc tgcccccgct cttaaatcag tagcgtttac tgctgcaccg tgtagctcat
    32521 ctacaccatt aacaaccgca cttcttccat ctatttttat attagcaccc attctattaa
    32581 attcagcaac atgcataaat ctgttttcaa aaacagtttc tataactaca ccagttccat
    32641 tggctacagt aagcatagcc ataaattgtg cttgaacatc tgtaggaaat cctggatgtg
    32701 gtaaagtttt tatatctatt ggttttaata cttttggacc tacaactctt acagaattgt
    32761 ccatttctgt tatttcacaa ccagcttctc ttaatttagc tacaactggt tttaaatgct
    32821 ccattaaaac attttcaaca gttatatcac cttttgtcat agctgccgca accatataag
    32881 ttgctgcttc tattctgtct ggaattacat tatgttctgc tccttttaac tctttaacac
    32941 cttttatttt tatagtatta gtacctgcac ctttaacatc tgctcccatt tcgttcaaga
    33001 agtttgctaa atctactatt tctggctctt ctgctgcatt ctctattatt gtagtccctt
    33061 ctgctaaaga agctgccatc attatgtttt cagttgctcc aacagatggg aaatctaaat
    33121 ataatttatt tcctactaac ttttctgttg ctgcttcaac aaaaccatgg tccatttcta
    33181 tctttgcacc taaagcttta aatcctttta agtgtaaatc tataggtctt gttcctatag
    33241 cacatcctcc tggcatagaa attttcgtac tattaaatct agcaagcaat ggtcccataa
    33301 ccaaaaaaga agctctcatc tttcttacaa gctcatatgg agcttcacaa gtttttatat
    33361 tgcttgcatc aacagtaagt gtattttctt tatattcaac ttctgctcct acatgtctta
    33421 ataaatctga tataacatga acatctctta aatttggtac tccatttaat gttgattttc
    33481 cgtttgctaa taaagtcgct gctattattg gtaaaactgc attttttgca ccatctattt
    33541 taacactacc tttaagtgga ttactctttt ttactattat cttagccata ttttttgatc
    33601 tcctcataat taataatcta attttataat tggtgtggca atatatatga gtgtttttcc
    33661 gtcatcctca ttatatttaa ttcctatatt taaattaatt ttttccccta aatattctaa
    33721 ttcataatca tttaatactt tactatatgc tgttatagat aaaaagttta cgtctttaac
    33781 ttcatctatt tgcttagcac tcatattata taatattttt tgtaaaatat catcatattt
    33841 gttgttttgt aacctttttg tgtattctcc aacaatacaa gcagacacat ctacttgaga
    33901 tgaatattta tttaacacat tttctacgtt actataaata acctctttat gtttatatac
    33961 tttgttatcc aatatgtcaa ctactatgta actttctttg ttatttttat ttattccttt
    34021 tatagaaatt tctttatcgc taccttttat agtagcacat aattgtattt tatcgccaga
    34081 ccctttttga ttccaattta ttcctttttt atccaacttt agattgttaa ttatttctat
    34141 aaaaatatta tgaaattctt tatttgataa attatgattt ataacagaat taattttaac
    34201 attataaaat ttaaaatttg cttctgatat ttcaagagct ttactta
//
LOCUS       DAEMIK010000025        33685 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEMIK010000025 DAEMIK010000000
VERSION     DAEMIK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.025"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            234..1076
                     /locus_tag="KPK87_001040"
     CDS             234..1076
                     /locus_tag="KPK87_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG2537211.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            1091..1555
                     /locus_tag="KPK87_001041"
     CDS             1091..1555
                     /locus_tag="KPK87_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537212.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1542..2714
                     /locus_tag="KPK87_001042"
     CDS             1542..2714
                     /locus_tag="KPK87_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2537213.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            2758..3693
                     /locus_tag="KPK87_001043"
     CDS             2758..3693
                     /locus_tag="KPK87_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG2537214.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3968..6661)
                     /locus_tag="KPK87_001044"
     CDS             complement(3968..6661)
                     /locus_tag="KPK87_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG2537215.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            6875..7573
                     /gene="yunB"
                     /locus_tag="KPK87_001045"
     CDS             6875..7573
                     /gene="yunB"
                     /locus_tag="KPK87_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG2537216.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(7785..8792)
                     /locus_tag="KPK87_001046"
     CDS             complement(7785..8792)
                     /locus_tag="KPK87_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG2537217.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            9020..9904
                     /locus_tag="KPK87_001047"
     CDS             9020..9904
                     /locus_tag="KPK87_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2537218.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            10106..11656
                     /locus_tag="KPK87_001048"
     CDS             10106..11656
                     /locus_tag="KPK87_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2537219.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            11685..12332
                     /locus_tag="KPK87_001049"
     CDS             11685..12332
                     /locus_tag="KPK87_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG2537220.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(12474..12734)
                     /locus_tag="KPK87_001050"
     CDS             complement(12474..12734)
                     /locus_tag="KPK87_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG2537221.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKNCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            12951..14234
                     /gene="hflX"
                     /locus_tag="KPK87_001051"
     CDS             12951..14234
                     /gene="hflX"
                     /locus_tag="KPK87_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG2537222.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            14242..14919
                     /locus_tag="KPK87_001052"
     CDS             14242..14919
                     /locus_tag="KPK87_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG2537223.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            15005..15448
                     /locus_tag="KPK87_001053"
     CDS             15005..15448
                     /locus_tag="KPK87_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2537224.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            15753..16397
                     /locus_tag="KPK87_001054"
     CDS             15753..16397
                     /locus_tag="KPK87_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG2537225.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(16483..16860)
                     /locus_tag="KPK87_001055"
     CDS             complement(16483..16860)
                     /locus_tag="KPK87_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG2537226.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(16860..17246)
                     /locus_tag="KPK87_001056"
     CDS             complement(16860..17246)
                     /locus_tag="KPK87_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG2537227.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            17425..18180
                     /gene="nadE"
                     /locus_tag="KPK87_001057"
     CDS             17425..18180
                     /gene="nadE"
                     /locus_tag="KPK87_001057"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG2537228.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            18263..18982
                     /locus_tag="KPK87_001058"
     CDS             18263..18982
                     /locus_tag="KPK87_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2537229.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(19149..20153)
                     /locus_tag="KPK87_001059"
     CDS             complement(19149..20153)
                     /locus_tag="KPK87_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG2537230.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            20419..21315
                     /locus_tag="KPK87_001060"
     CDS             20419..21315
                     /locus_tag="KPK87_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG2537231.1"
                     /translation="MNKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATIFGCPFEGLPKIEDAIRFAK
                     RLWENDIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHINIKSDSCNC"
     gene            21453..22715
                     /locus_tag="KPK87_001061"
     CDS             21453..22715
                     /locus_tag="KPK87_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537232.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            22791..23984
                     /locus_tag="KPK87_001062"
     CDS             22791..23984
                     /locus_tag="KPK87_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG2537233.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            24613..25407
                     /locus_tag="KPK87_001063"
     CDS             24613..25407
                     /locus_tag="KPK87_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG2537234.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            25502..26842
                     /locus_tag="KPK87_001064"
     CDS             25502..26842
                     /locus_tag="KPK87_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2537235.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            26979..28211
                     /locus_tag="KPK87_001065"
     CDS             26979..28211
                     /locus_tag="KPK87_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG2537236.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            28251..29393
                     /locus_tag="KPK87_001066"
     CDS             28251..29393
                     /locus_tag="KPK87_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG2537237.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            29404..30198
                     /locus_tag="KPK87_001067"
     CDS             29404..30198
                     /locus_tag="KPK87_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2537238.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVKDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            30209..31231
                     /locus_tag="KPK87_001068"
     CDS             30209..31231
                     /locus_tag="KPK87_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2537239.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEIVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            31330..32706
                     /locus_tag="KPK87_001069"
     CDS             31330..32706
                     /locus_tag="KPK87_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG2537240.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(33038..33382)
                     /locus_tag="KPK87_001070"
     CDS             complement(33038..33382)
                     /locus_tag="KPK87_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537241.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT        12510 a         4124 c         6148 g        10903 t
ORIGIN      
        1 tatgaatgaa taattttatt caattatttg aaaatccgtt attttaacaa tataaattaa
       61 taaattattt gtattttata tcatgaatct ataatatttt tttgattcat aactaaaagt
      121 atataaaaat gatgaataaa tatatattat acattatcaa tgataggtta tataataaaa
      181 tcactaacaa ttgtattata agaaaattat aaaaattaca aggaggtata gaaatgaaag
      241 aaaaagcagg gattagaaat gccataatag cagcctttat aatcacaata ttggcagaat
      301 taattggacc tatttcattt aaagttatgg gaattaatgt aactttactt agtatactat
      361 gggctatatt cataggaatg gctgtttctc ctcatttatt agggagagtt ataccagcct
      421 taaaaaagtt tataggggat aatgaaataa atgtatctcc atatttatta tctttgacac
      481 tatatccatt agggataatg tttggaatta atgcaggacc taaagttggt atattacttc
      541 aagcaggacc agcactttta ttacaagaat ttggtcatat gggtacaatg ttgatagcat
      601 tacctgttgg agtagcatta ggtcttggaa gaagtgcatt aggtggtact ttttcattat
      661 gtcgtgacac agcactagga attattggag ataagtatgg attaaactca ccagaaggta
      721 taggaacact aggaacttac ataagtggta gtatatttgg aacattatta ttttcatttt
      781 tagcaccagt gggtgtactt ttaggatttc atccatatgc tttagcaatg gcatcaggaa
      841 tgggtagtgg ttcaatgatg ggagctgcaa cagcatcatt aatgaatacg gttccacaaa
      901 tgtcagagca gattttagca tattcagcta ctagtggatt gattacagct gttactggaa
      961 tatatataga attattctta tctttaccag tagccaacta ctattatagc aaagtagctc
     1021 cagttattga aagatttaga ggaagaaaaa caagaagaac aagtaataaa ttgtaaagga
     1081 ggatatatat atggaaaaaa cttttaagac tattatggag cagataaaaa tagtattgtt
     1141 tatatatatt attatttgct ttggacaaat gatagcactt aagactcagg ctcaagattt
     1201 gttgattgga agtgtatctg gatttttgtt ggtgataatt gctattataa taaagaattt
     1261 tgttaaaaaa cctaatttac caggttttgc atgggctaca ttagttgcat tcgttttatc
     1321 gcttccaata tctccagtgg gggatatcat agcaacaaat gtagggaaaa taaattttat
     1381 gtctacagta acaccattat tagcttttgc aggaatctct gtaggtaacc aattagatat
     1441 attaaagact ttgagttgga agttagtagt tatatcattt atagttatga catctactta
     1501 ctttggttct gcttttatat ctcaaacagt tttaagatta aatggaatgc tttaaggagg
     1561 tagtaaatat gaatgaatta aaagcaaaaa ttaaagagga aataaagtta ttatctgaag
     1621 agaaaaagac atcatttgaa aaagtttctg actatttatt ttcaaatcca gaattagcat
     1681 ttgaggagta taagtcacaa aaagcattat gtgatttgtt agaagaaaat gaatttaatg
     1741 taactaaagg tgtaggagga ttagaaacat cttttgaagc tgtgtattca aatggaacta
     1801 atgggaaaac agttgctttt ttagctgaat atgatgcact tccaggaatg ggacatgcat
     1861 gtgggcacaa tatcataggg acaagttctg ttggtgcagg tatcatatta aaagaaatca
     1921 tgaaaaaaca taatatagaa gggactgtaa aggtttttgg aacacctgca gaagaaagag
     1981 taggtggaaa aataactatg attaaagaag gtgtatttaa taatgtagat gctgcattaa
     2041 ttttacatcc aagtgatgct tcaatgcctg atgatatatc atttgctcaa gttaatttaa
     2101 aatttgattt tactggtaag gcatcacatg ctgctgcttt tccttgggaa gggaaaagtg
     2161 ccttaagtgg agtaatagca ctatttaaca gtgtaaatag catgagatta catcttaaag
     2221 attatgcaag agtacatgga attataactg atggaggtag catacataat ataattcccg
     2281 aaaaatctac agctatattt aatgtaagag ctttaagcat agaatattta aatgagattt
     2341 gtgaaatgct taaaaattgt gctaaaggag ctgcaatttc tactggaact aatgtagaaa
     2401 tagttcaatt agatgaaata tacaaagaga ttaagaatga ttctgaatta gtgaatatag
     2461 ttagacaaaa ttttgaagtt ttgggtgaag actatgttga aagagattta agtcaaggta
     2521 taggttcaac tgatacagga aatttaactc atgagatacc tgcaatacaa gcatatataa
     2581 aattaaaaga aaatactgca actcatacag atgaatttgc tgttgcagct ggtggtgaag
     2641 aaggcaaagt agcacttata aaagcaatta aggtgctagc aatgtgtgga gtagatattt
     2701 tgtatagtaa ataatatata attttaaaat atattaagaa aggaaatcag attagacatg
     2761 atattaaaac aagtaataga ggtttatgac attttagata aagcaaatgc taatggggaa
     2821 gaagtaaaaa cttacttaca aggttatggt gaagtagatg taactgtaaa agaattgtct
     2881 tcaagtaaag gctcaacaga tttagtaaag cttacaattc ctgggaaaaa tggaaaatta
     2941 aaaggtggag atgctcctac tcttggcatt ttaggtagac taggagggat tggagcaaga
     3001 ccagaagtta taggttttgt ttcagatggt gatggagcat tggtagcaat tgcagtggct
     3061 gctaaacttt tggatatgca acgaaaaggg gatatattag atggtgatgt tgtaatttca
     3121 acccatattt gcccagatgc tccaactaaa gaacactatc ctactccttt tatggattct
     3181 ccaattgata tgatgactat gaatgaaaat gaagttgata gtagttgtga tgcaatactt
     3241 tctatagata ctactaaagg aaatagagta atcaatacta gaggatttgc aatatcgcca
     3301 actgttaaag aaggctacat attaaaaact agtgataacc ttttagatat tatgcagact
     3361 gtaacaggga aatctccatt tgtattccct ttgtctattc aagatataac accatatgga
     3421 aataatctgt atcacttaaa tagtatttta caaccagcag tagctacaga tgcaccaata
     3481 gtaggagtag caattacaac agagatgcct gttgctggat gtgctacagg tgcaactaat
     3541 tttagtgatt tagattcagc tgggagattt gcaattgaag ttgccaagct atttggtaga
     3601 aataagtgtg atttctatga taaagaagag tgggaaatgc ttattaagag atatggcaaa
     3661 ttaaatagat ttcaaacatt tggtatacag taattagaaa aatattaagt ttatataatt
     3721 tgtgatataa ctatttaaga atatataaac aattaggaat aaaagtaaga ataaagcact
     3781 tagttaaaaa ctttaaaaat aaaatattta tttgtttata taaaaaagga ggattctggt
     3841 tattgggatt ctctttttta tgaatacaaa taatatattg taattataaa aaaaggagcg
     3901 tttcaaagtg atttttttaa tcaccttgag acaactcctt atatttttat ttaaattcta
     3961 ttttttatta agttgctgga gttccaccac catcaggagg agtagtagag ccaccaccat
     4021 tatttccacc accatcagga ggagtagtag agccaccacc attgtttccg ccattatcag
     4081 gaggagtagt agagccacca ccattatttc cgccattatc aggaggagta gtagagccac
     4141 caccattgtt tccgccatta tcaggaggtg tagaaccgcc accattatca cctgtgttat
     4201 tattgttatt attattttct tctggttggt tattgtctgg tttttttgta tcttcagatg
     4261 atggttgaga acttacttta gtaggagcag taccttcaat aaatgctgct ttagagccag
     4321 aagattgctt tcctccatcc tctaaattaa tttttgtaaa atatacattt ttaggaactt
     4381 caaattcagt aaccgttaaa ttagcatgta ttttttccat aattttttcc catagttttg
     4441 cagtactagt acttccatgt ggtacttctc tacgttttat agtgttacca gaatcatctt
     4501 ttctaccagc atcatctcca atataagttg caccaacata ataaggagtg taaccgacaa
     4561 accaagcatc atatgcacta tttgtagtac ctgttttacc tgctacaggc attccctttg
     4621 gaagtgtagc aacaccacca gtaccttcag taattacgct ttctaacata tctgtaacaa
     4681 cataagctac ttcagggtct acaactttat gttcttcagg agcattttta actaataatt
     4741 gaccatcaaa ggtagttata gtagtaaaaa tagaaggttc tacataaata ccgtgatttg
     4801 ccaatgtacc ataagcagca gccatttgaa gtggagtaat accattcgcc ataccaccta
     4861 gtgtcaatga tgggaatttt ctatcacttg cttcagatga acctgagtga ttattcttta
     4921 aagttgttat accaaagttc tttaagtaat ccatcattat atctacacat tcatcataag
     4981 agtctccaag catttcagca gttttgacag ctatcgtgtt tgaagattta gcaagtgctt
     5041 tacgtaaact catagaacca tgaccagcag tagtagttct tggattccat ttattatttt
     5101 cttcaaactt atatcctcct cgagaatcac ttagagcagt tgcttgtgtt atttttagag
     5161 tatcaatagc tggcgtatat attgctaaag gttttataga agaacctggc tgatgagggt
     5221 tagtagcacg atttaaagtt tttctaccac ttatatttct tcctccagct agtgctctta
     5281 tttcaccatt tttataatct aagattacca ttgcagcttg cggttggcta actccatcag
     5341 gaccgaccat atgtcctggg aaattgctat ttctatcaaa ttcttcttct aaagcatctt
     5401 gcattttagg gtctatagtt gtgtttacaa ttagaccacc attgtagaat aagttttgag
     5461 cttcatcatt tgtatgtcct tgttccatta aagcatttat aacttcactt tcgataaggt
     5521 cttctacaga tgaagaaact ttatctgatg attgaggcaa ttttatttct attttagctt
     5581 tgattgcttc atcatattca ctttgtttta tatatttcaa ttctaacatt ttctttaaaa
     5641 caatttcttg tcttttttta gcttcaggat tacttacagc ctttctaaca actaatgtac
     5701 ctgcttttaa ttgtctataa gtatcattag atataagctc ccaagatttt attttttcaa
     5761 tcatatcaaa gtccacttga gtagggtcat ctaaatcatc atcagttgtg ttaacaaaaa
     5821 ataataattt attctctaaa tcctccttag tttcatttcc ctctaactta gaagttttat
     5881 aggctgaaaa tcttgatgga tttttagttg aaccagcaag aagtgctgac tctgctagag
     5941 taaggtcagc tgcagactta tcaaaataac cacgtgcacc agcctcagca cctgctagac
     6001 ctctaccaac gaagaagtta tttaaataag tttctagtat tttatcttta cttactgttt
     6061 tactcatctc atgtgcatag taaatatcct ttattttacg aggtatagtt tgctgttcag
     6121 ttgttaacag cattttggat acctgcatag gaatagtact accaccttgc ttagtacctg
     6181 taagggtctt taatactgac ctaaataaac ctttaatatc gacaccctta tgctcataaa
     6241 aacgctcatc ttctattgca attacagcat gttgaagata aggggaaatc ttatctaatg
     6301 gaacgggtgt ctttttattt acacttggag ctgtagataa tgtttttcca ttagcgtatt
     6361 taatagtagt tgtttggttc gtctgtttat ctaaaacagc ttctgtaact ggagttacat
     6421 ctcttagtga cgcaaatact agaccagcag tacctgctgt accaacaact agtaaaacta
     6481 aaaatactat cccaaatact cttagttttt taaatttatc gcttttttta gattttttct
     6541 tgttttttgt tgaacgagtc ttgttagctg aagttctatt aattggttta tttgtattac
     6601 cagatgagct aacttttttt ctcctgattt tatttccatt tttatcatta tcgttattca
     6661 taagatttta acctccttac ttaatgcaat attataacac aaataggagt agttttgtaa
     6721 ttaatagaaa aaatgaattt gctataatat tctgtacatt gtatatgtat aaacgtaata
     6781 tattaaaaag tctatatttt tattttattt atttttgaca aatattataa aaattatgcc
     6841 aaagctacat ataaataaat aggaggggat aattttgaat agaatagtag atagaaaaac
     6901 aaaaaatgaa tttagaaaaa tcatagctat ttttttagta attcttttct tgtctgtatt
     6961 tataggcagt tttatatata tagataaaac tttaagaccg acaataacag ttttagcaga
     7021 gactaaagct cttgaactag caaataggtc gataaataaa gctgttgctg agatggtaga
     7081 aggaaaaata aattatgaag atttaatgga tatacaactt gataataatg gtaaaataac
     7141 tatgatacaa gcaaacacta taatgatgaa tgaaattgca tctgcaatag ctttagaaat
     7201 acaagatgaa ttaaaaaagg ataagacagc aagttcttac atacctatag gaacagcatt
     7261 aggaagtcct atattagcta aatacggtcc taaacttgaa gtatctattg aacctatagg
     7321 aacagtatca gtaaacttta aaacggaatt tgagtcttca ggtataaatc aaactagaca
     7381 tagaatatat ttagaagctc aaacacaagt taaagtagta ataccactta taacttcaac
     7441 aaaacaaatt aaagctcaaa tacctatttg tgaaactata atagtaggtg atgttccaga
     7501 aagttatgta aatattccag aaaaaaatct aggaaatgtt ttacctaaca ctggaaagaa
     7561 taccaacaag taatgtaaag ttgtatattt ttaaataaat tcaaaataat agtttataaa
     7621 taaaattttt ggaaataata ttaatataat tttaataaaa atttatattt tatactatgt
     7681 aaaatatcta atatatttgg tctgtaggtt aaataaaagt acctttagac caaatatatt
     7741 attaataact taataacatc ttaaaactta tttatttata ttatttacaa catccattta
     7801 ataaatacac cataacctac agctgggtcg ttttcaattg catgtgacac tgctccgatt
     7861 attttattat cttgaactat tggagtacca ctcatacctt gtacaatacc accagtttga
     7921 gctaaaagct ttttatctgt aattttaatt ttaaaacttt tactttctgg atgtttttgg
     7981 ttttctatat ctataatttc aatattatat tttttacact tattaaagtt atcttgtaaa
     8041 attatttgag cttttcctaa tttaacttca tctaaagatg ctactttata tttttttaac
     8101 ttggaaatat caaagttatt aatttgacct cttattccaa aatcagtatt ttctgtaaag
     8161 ctaccaataa tattttctcg ttttgcattt atacatccaa cactaccttt ataagatttt
     8221 tctatattaa ggtgcttagt tgtagaaatt gaacctgttt taataggtat ttttcttgca
     8281 ttaccaacac ttattggatg acctacagct ccaaactctg atgtatctgg attgatatac
     8341 gttaaagtag caaatccaga aagtaaattg ttaaaactaa ttttttctaa tatgtgtttg
     8401 gtagttttca ttgttataag ttgtccacct ctatttaata aaatagatac ttgtttttct
     8461 tctggtaaag catttaaggt attggcaaag tcactatatc catcaacttc agcattattt
     8521 acctttacta tagcatcacc caatctaaat ggagatgctt ctccaggatt tctaactatg
     8581 atatttttta attctgcgtc aatttgaagt acgtttccaa ttggtattaa atattcaggt
     8641 tcattttttt gtaaattttg tgcataaatt aaattatttg agaaaaaata taataaaaat
     8701 aaaaatgtta aaacaattgt aaattttgac ttagctaagt tggttttttt acttaaagta
     8761 ttatttttaa aatgaaaatt tttaagttgc ataaaacacc tctttcttat taaattattt
     8821 taataataga attgaagttt tcttgctgct tctatattat gttgtgcaag aatcatgttt
     8881 tgatgcttgg aaaactagct caataacacc ttagttttca taaaaataaa taaaataaat
     8941 agtgtatatg ataaaataaa tacatattca tataaataaa aataaacaat aactgtctag
     9001 cataagaaag gagcaaaaaa tgaaaaaata tagattgtta gttacttgta taattgccat
     9061 gggaatcata attataggaa catcaaatcc agttagcaat ttttacaaga ctacaaattt
     9121 gattagttca ggaaatatca caaatgaaaa agcagataaa gatgaaaatt caaacaaaaa
     9181 taataaagat aaaaatgaag acaaatctaa ttctaaaagt aataaagata agtcatcaga
     9241 ggacaaatcg tcagataata aaaatgaaat tcaaaaaaat aaaaaacttt taatttgtat
     9301 agacccaggg catcaaggaa aaggagatag caatttagaa ccagtagctc caggttcttc
     9361 ctctaaaaaa gcgagagtat cttcaggaac agagggtatt gctacaaaaa agccagaata
     9421 tgttttaaac ttagaagcat ctcttgtttt aaagagtata ttagaatcta aaggttataa
     9481 tgtaataatg actagagaga cacatgatgt aaatataagt aattcagaaa gagcaatact
     9541 tgctaatgac aaaaaagcag atatggttgt aagaatacat gcagatagtt taaataattc
     9601 ttcaaaaact ggagcatcta tattgattcc tgaaaaagat ggaaaatata cagctcctat
     9661 atatgaagaa agtaataaat gtgctgaatt tatcaaacag aatatggaac aatcaggtat
     9721 acaaatcaat ggaatcgttc aaagaggtga tttgactgga ttcaactggt caaaagtacc
     9781 agctgtatta gtagaaatgg gatttatgag taactataat gaagaccaaa tgatgtcaaa
     9841 tccagactac caaagaaaaa tgatgcagtg tatagctgat ggattggatg cgtattttaa
     9901 ataagatatg aatagagaaa aagttaggtt ggtattattt gcctaatttt tatctctatt
     9961 ttttattgta cataacacaa agaagaagtt tattttaggt taaattgtcg taaatataac
    10021 taaaaatatt gggaatactt gatttaagtt taaaaagaat atattatagt atagaatgtg
    10081 taaaattttt ttgagaggtg ttgaaatgct agtagtagaa aatgtgagtc atggatttgg
    10141 tgcgagaaca atattagaaa atgtatcctt tagacttaga aaaggtgaac atattgcact
    10201 agttggtgct aatggagaag gaaaatcgag ttttttaaat ataataacaa agaagcttat
    10261 gccagatgct ggaaatataa aatggtcttc aagagctaca gtaggttact tagaccaaca
    10321 tacggtatta agcaagggaa aaactataag agaagtgctt agagaggctt ttaagcatat
    10381 gtttgactta gagcaagaaa tgattgctat gtatgacaaa atgggagaag ctagtgatga
    10441 tgaaatgagt aagcttcttg aagaaacagc agagatacaa actatacttg aaaatagtgg
    10501 tttttatatg atagatgcaa agatacaaga agttgcaaat ggtcttggtc ttggagaaat
    10561 tggtcttgat aaagatgtaa ctgacttaag tggagggcaa agaacaaaag tacttcttac
    10621 taaacttcta cttgaaaatc caactattct tatattggat gagcctacaa actatttaga
    10681 cgaagagcat ataacatggc ttactaagta ccttcaagag tatgagaata gttttgtctt
    10741 agtttctcat gatattgaat ttataaataa tacatgtaat gtaatatatc atatggaaaa
    10801 tggagagtta aatagatata aaggaaatta tgatgaattt gtaagactta atgatataaa
    10861 gaaacgtcaa gaagaacaag cttatgataa acaagttgaa gaaagaaaaa gactagagga
    10921 ttttgtagct agaaataaag ctagagtagc aactagagga atggctaaca gtaggcaaaa
    10981 gcaacttgat aagatggaaa tattagaaag accaaaagaa aaaataaaac ctacttttgc
    11041 ttttaaagat gctagagcag ctagtaaaat aatttttgaa acagaaaatt tagttttagg
    11101 gtatgatgaa gcattgacaa aaccacttaa tttccattta gaaagaggaa aaaagattgc
    11161 cttaaaagga atgaatggta ttggaaaatc taccctatta aaaactttac ttggaataat
    11221 aaaaccattt gaaggaaatg ttaaattagg tgactatttg gaagttggat attttgaaca
    11281 agagagttca agagaaaata gtaatacacc tatggatgaa gtttggagtg aatttccagg
    11341 gcttacaaac tttgaagtta gacaggctct tgcaaaatgt ggacttacaa atgaacatat
    11401 aacgagtcaa atgagagtat taagtggtgg agaagctgct aaggttagac tttgcaaagt
    11461 aatgttaaaa aatataaatt tcttagtatt agatgagcct acaaatcatt tagatgttga
    11521 ggcaaaagat gaattgaaga aggctatcaa agagtttaag ggaacagttc ttttagtatg
    11581 tcatgagcca gaattttatt cagaaattgt tgatgatgta tggaatatag aagattttac
    11641 aactaaaatc gtgtagtgat ttaacttatt gctgggggga tttcatgaaa aagtttctaa
    11701 atgtactttt ttcaatttgt attagtattg ttataatagt aggtgttatt aattttactg
    11761 ttggatttaa acaactttat tattttgaca ttgattactt aaatatatca gagttatctg
    11821 gtttatctaa agatgatata aaattgaact acgattatct aattgattat aacttaaata
    11881 agaatgtgtc tgagttcaaa ttaccaacat taaaatcttc tccacaaggg aagatacatt
    11941 ttgaagaagt caggaatata tttcaaaata taaataaatt agctaaatta ttattggtag
    12001 tttctttagt aggaattata ttgagtgtaa aaaataaaaa tataaaaatc ttaaaaacta
    12061 catcaataac acttataatt atgccattat tattaacagt tccaatttta ttaaattttg
    12121 aaaagtcatt tattattttt cataaattat tatttagaaa tgattattgg atatttaatc
    12181 ccgatttaga cccagttata aatatattgc cagaggaatt tttttttcac tctggaatga
    12241 tgattttaat attaattttg ttagtaagta tagtactgtt tgtaatgtat aaattatgta
    12301 aatctttatc aataaaaata tttcaatgtt aaatagaggg atatttttat ttccaaaata
    12361 agataataaa aaaggattag attaaaagtt atacaataaa aatagctgtg caataatatt
    12421 tagactatga aagtagttaa aaaatattta tgcacagcta tttataatag aatttacttt
    12481 ttactagtga ttttaactaa atcttgaaat ttactcataa tgtaattgta attatctttt
    12541 ttatgaggta atatacaact actgcaattt ttgatacctt tatctgtata cctaaagttt
    12601 ccaccacagt tttctcctaa agcatacaaa ggacaatagc aaaatagaca gttaaactct
    12661 tctggtttat tagttttatg acaagggaaa aattcgcaat ctttgtgatt aaaaaactta
    12721 taattttcgc ccatattttc ctcctaatta aataaaattt aaatttttta tactatataa
    12781 gattttggtg ataatataat tatactaaat aattttatga atagtcgata atatagtatt
    12841 attaatcata gtattaatgg tataattagt caacagaaaa ataatgtaaa aataatgtaa
    12901 gtgtattttg taattagaat atgttttata acaaaaaatg aggtgattaa gtgaaagaaa
    12961 ttcaagaaaa agcacttctt gtaggtctga atttgactac aatagtgaaa aaaaatgatg
    13021 atatagacac taatgaatct atggaagaat taaaagaact aacaaaagct gctggggcag
    13081 aagttgtagg tagcttaatt caaaataaac actctgtaga tgcagcttat tatataggaa
    13141 aaggtaaagt agaagaaata agagcctata gtgactcttt agatgctact ttagtaattt
    13201 ttaatgatga attatctggt gctcaaatca gaaatataga gaatgttgtt ggaagaaaag
    13261 taatagatag aactacttta attttagata tatttgctca aagggcactt agcaaagaag
    13321 gtaaattaca agttgaatta gcacaattaa aatatagatt gccacgactt tatggaatgg
    13381 gtggtgagat gagtagaaca ggtgcaggga ttggtactag aggtcctgga gaacaaaaac
    13441 tagaaataga taagagacat atacttaata aagcagcaga tataagacgt gaacttaaag
    13501 aagtaaaaaa gaatagagag acacagagag ttaagcgttt aaagtcaaat ataccgattg
    13561 tagcacttgt tgggtataca aatgcaggaa aatctaccct gttgaacgaa ttaataaaga
    13621 cacacaagga ttatgaacaa gaaaaagaag tttttgtaaa agatatgctt tttgcaactt
    13681 tagatgtaac tttgagaaag gctcttttgc cgaataaaaa agaattttta gtagtagata
    13741 ctgttggatt tgtaagtaaa cttccccatg atttagtaga agcgtttaag gcaacattgg
    13801 aagaagtaca gtatgcaaat ttaatcttac atgtaattga tgcaacaaat actagttatg
    13861 aattgcaaaa aagtacgact gaaggtgtat taaaagaact tggtgtaaat gataaaaaac
    13921 atatacttgt atataataaa gtggacaagc tagaattgga tatataccct aaaagtcaag
    13981 aagatattgt gtatatttct gcaaaacaag gcataaatat ggataagtta ttaaatatga
    14041 tagaaattgc tttaatggaa aatacttatt cagtaagttt gatgttacct tatgagagag
    14101 gtgatatatt tagtagaata aaagataaat ataatgtgga gaattttgag tatggggaaa
    14161 gtggtataac gctagatgca gatttagatg aagaagattt taatatctat agagagtaca
    14221 tactagaaaa ataggttgaa tatggatata aactgggaaa atatagataa tcttgaagat
    14281 tattttataa cctatcttct ttataaagaa tctaaaacag tatctcaaat tagtaaaatt
    14341 agaaatattt caagtactga agcaagtgag caacttatac aagcaaagtt aaaaataaag
    14401 gaaatgcaaa aagatgattt tgaggcatct aaggatattt tggataaatt tctagaacta
    14461 gataaaatta agagacttga ttttatggat agcttagatg acgaaaaaat ggtatacttt
    14521 aagagaaagg ttttcaaaag aatccttgta gaaaaaaatg ctgaagattt aattgtttta
    14581 atttgggcga caggtgagct aaaagatgat aggtttttga aattgcttca tcagctcaca
    14641 aaccacagac actcagatat tagaagaata acttattcag caatcagaaa gattgaatca
    14701 ccaagtagta gagaagtact gcaaaaaggt ctctacgaca aaaatgcaca gactaggcag
    14761 tactgtgcaa aagctctttc aaaattggga gatgaaaaca gtttaaagat tttacagcag
    14821 ttgaaagaca agaataagaa ttttgaaaaa gaatatgtgc ttagagctta tgacgaagcc
    14881 attaagtcat tggaaaatgc aaaaaatttg aaaaagtaat tttttattaa agcaatttgt
    14941 ttacttaatg atgtatttct aaagtttaac aaggtaataa ataaattaat gggaggggtt
    15001 tcaaatgata gtaagacgtt gtgcaggtgg agtagtgttt tatgctaata aagtacttat
    15061 tgtaaaaaat gataggggtg aatggacact tccaaaagga aagatactag gagggggact
    15121 tccatatgag agtgctgttc aaagagttaa agtagaaact ggcatagatg ctaaaatgat
    15181 agatgtggct ggcgatacta tgtatgaatt tttctcaaga agtagacaac aagaagtttg
    15241 taatgcaata atgtggtatg ttatggaagc ttgtaacact gattgtgtac ttgctcctga
    15301 atttcaagaa ggtggatttt ataaagtaaa ggatgcttta gaaatgttat cacatcataa
    15361 agagcaagct ttagtagaag tatcttataa aaagtttaag gaattaaaga aattagtttc
    15421 agagggcgat gatgttccag caaattaatt gtgaatttag aggattctga ggaagggata
    15481 gccccttcct tttttaattg aagcagtttt aagcgtaaat tatatagtct ctttaaacta
    15541 tgatttatca atttagagat ttagtagttt atatttaaaa tcagtaagat atatttaaaa
    15601 taaaaattaa aaagtataca attttattat ataattgtct tatgatgttg attaaagaat
    15661 tattaaaagt ccatttattt ttaaatagaa taattttata aatatatatt tgatgtgtat
    15721 attagaaaat gattaaaata ggaggaaata caatgagcaa ttacaggaca ttacatgaat
    15781 ttggaacgga tgaaataacg attgaaaagt ctgtttttat tggatatgct aagcctataa
    15841 agagtgaaga agaagcatta gagtttataa atgaaataaa gaaaaagcat aaagatgcaa
    15901 ctcacaatgt gtgggcttat actgtaggta aaaatatgaa tatacaaaga tatagtgatg
    15961 atggagaacc acaaggaaca gctgggatac ctacattaga ggttataaaa aaagaagatt
    16021 tgagagatgt agcggttgtt gttactaggt attttggagg aacaaaatta ggtgcaggtg
    16081 gtctagttag agcttataca aaaggtgcta aactgggact tgaagcaggt aagataattt
    16141 ataaggtaat gtatcaagaa gttaaggtta agatagatta tactcaactt ggaaaagtgc
    16201 aaaatgagtt aatgaatttg gggtatttta taaaagatac tgtatatgag gataatgttg
    16261 aaatagtagt atattcaaga ttagaagatg tagagaaatt atcagaaaaa atgattgata
    16321 taacaagtgg aacaggaaaa atagttttag gagaggaatt ttatttgtct gaacaaaatg
    16381 gagaaatttt aatataatct aaaaggttat tagaatagtt aatacctcaa atagccaatt
    16441 agtattttta agtgaaaagc cattaaattg atttaatcta aatcaattta atggcttttt
    16501 atcggatttt gcacagaatc ctttttttac ttcaaataaa ccgactaaaa gtaaaaatat
    16561 cccaaatatt ttttttaata tatctgaaga cagataatta gctaatagtg aaccaaatac
    16621 agctcctaaa accccaaaca cagctatagg tataaccaat ttaaaaatta catttttatt
    16681 ttttatatgt atagcaagtg ctataagtgt catagggata gatgaaagta aatttacgct
    16741 ttgtgctatt ttagtgtcta tgccagcaag taaaacgagt gcaggtataa ggattgttcc
    16801 tccacccatt cccattcctc caataattcc tgcaaaaaat ccaattagag caaataacat
    16861 taaaacacca tccttaatgc tgcaattatc ataattattc caaaagaaat tctaagaaac
    16921 tttccagtaa ccttatttag taatttagct cctataatac caccaagaat acttccaact
    16981 gcaactttaa ttgttaagtt aacatcataa gtacctttag atacatataa aacagaactt
    17041 gctgtcgtta agaatattat tatagaaaga gcagttgcat gagatttatg ttcttctacc
    17101 ttaacgatgt catttaatat tggaactaaa agagtacctc ctccagaacc aaaaactcca
    17161 tttataaatc ctgtaaaaac tccaattatc gtatttttta agttaaaatc aaagtatttt
    17221 ttctttaaat catcagtttt tttcataaaa atcaccttct gaaatagttt taccaaaata
    17281 ggcaaattat atttatataa aataagatta aatataatct ttcatgtttg tttaattttg
    17341 cataaaaaaa aacatattaa aacaacatat taatatatgg tataatatat taaaacaaag
    17401 tagcaaattt gagaggagat aacaatgtca aatataaaaa tacaaataga caaaactgta
    17461 gaatggctta taaataaggt taatgaggca aatgcaaaag gattaattgt tggtgtatct
    17521 ggaggaattg attcggcagt agttgctaat ttaattaaaa aggcatttcc agaaaactct
    17581 atgggagtta taatgagcat aaaaagtaat cctcaagata gagaagatgc acttaaggtt
    17641 atagagggat gtgatataga ataccttgat ttagacctta tagaaccaca aagtgctata
    17701 ttagacatgg tagtaggaaa tttaaaagat aaacatttat atagagaaga atatttaaag
    17761 atgacagatg caaacttaag agcaagagta agaatgagta caatttatac aattgctaac
    17821 aatctaggat atctggttgt tggtacagac aatgctgcag aaatacacac aggatatttt
    17881 actaaatttg gtgatggtgg agttgatatt ttacctattg caaatttaac taaaggagag
    17941 gtgtatgaat gggcaaaaga gcttggtgtt catgaagatt taataaacaa agctccttca
    18001 gcaggccttt gggaaggcca aacagatgaa gatgaaatgg gaactactta caatatgata
    18061 gatgctgtgt tagaaggtag attggatgaa gttcctaaaa gagaccaaga gataattgaa
    18121 agacttcata gattaagtga acacaagaga aaaacgcctg cacaacctcc taagttctaa
    18181 tttactattt ataagatttg tgataaaatt tacaagtgat gaaaattaga ataattaatt
    18241 gtacgtatta aggagtggaa atatgggacg tataggtaat ataattaata gaaaaggtaa
    18301 acaagatgct caaagagcaa agatatttac taaacatgct agagcaatag cagttgcagc
    18361 taaagaaggt ggagcagacc cagagtacaa tgcagcgtta aaaacagcta tagaaaaagc
    18421 taaggcagat aatatgccaa atgataatat agatagagct atcgctaaag gtgctggagc
    18481 aggtgctgga gaagactatg aaactatagt ttatgaagga tatggacctg gtggagttgc
    18541 tgttatagta gaaactttaa ctgacaacaa aaacagaact gctggtaacg taagatacta
    18601 ttttgacaaa aacggaggaa acttaggaac tagtggatgt gtgtctttta tgtttgataa
    18661 aaaaggacaa atattagttg gtttaggaga tggagtttca gaagaagaat tgatggatgt
    18721 tgcattagaa gcaggagctg aagatttcat aacagaagaa gatggttatg aaataataac
    18781 tacaccagaa gatttttcta gtgttcgtga tgagttaaaa gctaaaggat atgagtttat
    18841 atctgctgat gtgaagatga tacctcaaac tacaactgta ttgactgaag aatctcattt
    18901 aaaaatgatg aataaattag ttgatatgct tgaagaagat gatgatgttc aagatatata
    18961 tcataactgg gaagtagaat aaatagtata agcatattga aatttataag tatttaatca
    19021 attttaacat gtgataaatt ttatagaata agaaacacta aaatagacat acgcacctct
    19081 aacctatatt tgcataataa tatgtaagca taggtaaggg gtgtttttat atttataggt
    19141 taagaaattt aaagtaaatt ttgagctgga ctcgattgtc tataaataat tgatgggtat
    19201 ataacttgtt gctgaatacc atttatacgc ccagttactg tatcaataat catcctaaga
    19261 ctacgttctg ctgtctcttc catttttaaa tctataactg tcaaaggagg aatacttctt
    19321 gccattagtc gtgaaggtcc attacttgct gcaaatattt gaatatcctc tccaattaca
    19381 attcccgatt catgaaatgc attcatcata ccaagagcta ccacatcatg attacaaaaa
    19441 attacttttt ttagtgaatt ttttgcaagc attttttgag caatatcata tccactatct
    19501 attttattag aacaataata tatagaatct gatagatgta ttccatgttc tttacatgtt
    19561 tttaaaaaag atttacccct taaagtcata catgtaaagg aagttgggtt gaatacaaca
    19621 gcaatatcat ttttaccatt ttgaatagca tgtaaagctg ctatacgacc agtttcctca
    19681 ttatcaatag atacacttga aaaaccaggt atttcacgat ttacaacaac tacaggaatt
    19741 ttaggtttat tttttactag atattctata tcatcagcat tgatacctgt taaaacaatt
    19801 ccatcataat aattatttgc aaaaaagtat tcatcatcac atagatgacc aactttgtaa
    19861 ggtcgtatga tgatatttat gtttaaatgt tctaaagaaa ttatgtggtt tattgcttga
    19921 agtatattgg ttgatgccaa ctcaaatccc tcatcctgcc aataaaaagc tattttaata
    19981 ttattatgtt taataggagt agagcgttta tatccaagct tatcagctga atttaatata
    20041 agctcttgtg tatgtagcgg aatcctgcgt tcatttcctt taccacttaa gacgtacgag
    20101 acagttgctg aagaaagacc acagtctttt gcaatatctt tgattgttgc catgataaaa
    20161 tcctccttaa atagttgctt aaatttgctt ataccaataa tcttaacata tattcaacta
    20221 aagaacaata ttctacaagg tgcatatttt acaggattag aataaataaa tttaaaaaaa
    20281 cttcttactt atgttgacat aatttagttt ataggattat aatatgctta aataagctta
    20341 ttattaactt aaattaagca aagttaagca ggaacatgca atgctagctt gcaatgtgac
    20401 tgtaaagggg gaaaaggtat gaataaggta aatatatttg aagttggtcc tagagatgga
    20461 tttcaaaacc tgaaagagtt tttagaaact gataaaaagc ttaagattat tgatggtctt
    20521 attgcttcag ggattaaaca acttgaaatt agttcatttg taagcccaaa agcaatacct
    20581 cagatgaaag atgcaaaaga agtagcaact tattgtgtag aaaaatatcc tgatgtgaaa
    20641 ctttatgcat tagttccaaa tttatacggt gctaaagttg catgggagtg tggtataaga
    20701 gatatatcct atgttatatc tttgagtgaa actcataata aaaaaaatat aaatcgtacc
    20761 catgaagagt ctattgatga gcttaaaaaa ataatgaata cttatccaga catgaatata
    20821 tgtattggaa tggctactat atttggatgt ccttttgaag gtttaccaaa aattgaagat
    20881 gccataagat ttgctaaacg cctttgggaa aacgatatca gggagataaa tctagcagat
    20941 acaataggga ttgctgaccc taagcaagta agagaaacta ttagtgcatt agttgaagct
    21001 ttaccagagt gcagatggca agtacatatt catgatacta gaaatatggg aatggttaat
    21061 actcttgtgg ctattgaatc tggaattact actgttcagt ctacacttgg aggattagga
    21121 ggatgcccat ttgctcctgg agcatctggc aatactgcaa ctgaagattt agtatatatg
    21181 cttgaacgta tggggtatga tacaggagtt gattttgtta aacttcttga attatcaaag
    21241 tatcagaaga gttttgtaaa tggagtatat agtggtcatc atataaatat aaagtctgat
    21301 agttgtaatt gttagttcta ttgattaatc gattaaaaaa atatgttgat taaaaaaata
    21361 tgctgattgg taaaatagat ttattgataa aaaatataaa ttataaaata cattatctaa
    21421 taaaacaaat aaaaaaattg agggggatta gtatggatat tatagcttta cttttatttt
    21481 taggagttat tattattgca tttgtacgta agaataatgc aggtattttg gctctagcta
    21541 ttggtgtcat agctgttaga atttttggaa tggatgataa agaactttat ggagctataa
    21601 gcaactcaat gtttactaca cttgttggta taacactact ctttgcaata gtgaactcaa
    21661 caggagcatt aaagcttgtt gcagaaaaaa ttgtagctgc ttctggtaaa cgtgtttggc
    21721 ttatacctat agcagttttt atagcaggat ttgttgttgc tggtgttgga cctggggcaa
    21781 ttccagcact tgcaattatc ccagcaatgg ctgttcctgt agccataaca gttggttata
    21841 atcctgtaat gcttgcactt ataggtgaat gtggattaat ggcaggtcgt atgactccta
    21901 ttactccaga aggtcaaatt ataacaaccg ttgcagaagg ggttggaatt gaaaatgtta
    21961 tgcctactat cttagcctgt caaactcttt ctacaattgt attttcaata gtattgttta
    22021 taattttcaa aggatataaa cttaaaaaac ctataaatgt tatagatagt aagcaaatag
    22081 aaaaattcaa tataaagcag ataatttctc taataagcat agtagttatg attgctttta
    22141 tcataatttt tgatatgaat gttgggcttg cagcttttgc agtttcagct gtacttattt
    22201 ttttcaatat tgctgatgaa ggagaatgta tcaaatcaat tccatggagc acgatagtaa
    22261 tggttcttgg agtaggtgca atgatgacaa tcgtagatgc agcaggaggt attgacttga
    22321 tgagtaatgc attatcatca ctgatgaata gtaaaacagc tactccaatt atgagtattt
    22381 cagcaggtct tatgtctatg gttagttctg ctttagcagt agtttatcca actatgatgc
    22441 ctatgtgtgt tgatatagct aaggccgttg gaggagtaga cccactagca cttattgctg
    22501 cagttggtgt tggaggttct cttgctggtg taagcccact ttctactggt ggggcattga
    22561 ttttagcagc tatgggttca agtaagaaag actttaataa ggaagaacaa aataaggttt
    22621 ttgtacagct tttcattatg agtgcagttg gcttattggt tattgcagta gtatcaatat
    22681 tatcatttaa tgcaataaca catttaatga actaattaga ataactgaaa ataaatcatt
    22741 aaatttaact tttaatatta atcaatataa tataatttgg aggggaagtt atgaaaaaag
    22801 gagcattaag caatataaag gtacttgacc ttacaagagt tcttgcaggt ccatattgta
    22861 caatgatgtt ggcggatttt ggagctgatg ttataaaaat agagatgcca agaaaaggtg
    22921 atgatacaag aggttttgga ccttttgtaa atggagagag tttatactat gcaaatataa
    22981 atagaaataa aaaaggtatc actctaaacc ttaaatcaga agaaggaaaa aatatatttt
    23041 tagaaatggt aaaacatgct gatgttgtag tagaaaatta cagaccaggt gtaatggaaa
    23101 agttaggtct gggttatgat gagttggcta aagttaacaa tcaaataatt tatggagctg
    23161 tatcaggatt tggatgttat ggaccatatt cagaacgtcc aggttatgat attattgcac
    23221 aggcaatggg tggtcttatg agtataacag ggcaagaagg aggagaacca acgagagttg
    23281 gtaatgctat gggtgatgta ctaggtggaa tgaatttaac tattggtata ctcacagcac
    23341 tcaacgctag aaaaataata ggaaaaggac aaagagtaga tgtttccctc gttgattctg
    23401 ttgtatcgtc tctcgaaact ggtatacaga gatactttga ttcaggacaa cctgaactta
    23461 tgggtaatag atatgcttca gcctatcctt atgactcttt taaggcaagt gatggaagtt
    23521 ttgtaatagg ctgtggtaat gacaaacttt ttgaaaagtt atgtaaaaaa gttcttttta
    23581 gagaagacct tttaatagat gagagattta aaacaaatct taatcgttgt gaacattaca
    23641 aggctcttaa accagaaata gaaaaatggt ctgttaagca tacaataaaa gaagcagttg
    23701 acttaatatc agaggctgga gttcctgctg ctcctatcta taatcttgat tgtgttacaa
    23761 cagatgaaca tatagcaaaa gcaagagaaa tgattgtaga tattagtcat cctattatag
    23821 gaaatatgaa agtcaatgga aatccagtta agttgatgac aacaaaagct aaaattgata
    23881 tgccagcacc atctctaggg gaaagtaacg aggagattta taatggttgg cttggttttg
    23941 atagtgataa aataaaagat ttaaaagaaa atggagttat ttgattaaaa aatagctaaa
    24001 cacacttgtg actttagtca tgggaggttc agttagaata atatcaaaaa atattaaatc
    24061 aatactgtaa atcaaatata gaattgtcag aagcatttaa gatgggaaaa gctacttaga
    24121 cattgagtct ttgtggcttt tcctattttc attaagaatt ttatattatc agaatagata
    24181 tgtactaaac taaagtatta acaatttaaa ttaatagaaa ataaactacc taattatgtt
    24241 gaataaagac tactttagtg aaaataaact tatagagatg ctatttaaat tattttaatg
    24301 gaaatatcaa aaatgagact gcattctcaa aaatgagact gcgtttttta agtattttct
    24361 cgaaaatgag acaaaaatat tgttttatgg gttttattga agaaaaattc ttctgtgaat
    24421 tacaatttaa tcaattttaa agttgaaaat tattaaaaat ttagtgaaat ttatgtatat
    24481 ttcgtttatt ttattaaaaa taacaatttt ttactaaaaa taaagaactt caatggtgat
    24541 tggcatagta cttgctattt tatattaaca agaaatgtag aaaaaataac aatgtataat
    24601 ggggggaata tgatggaatc tgtaatggaa aatttgaaca atcttaaggt ggaggttaag
    24661 gacaaagtct gtgttattac tataaataga cctaaagctt taaatgctct taatagtgac
    24721 actcttaggg aactatcaca agttattgat gtagtatcag aaaatgaggc aatattagga
    24781 gttattatta ctggagaagg taaagttttt gtagccggtg ctgatatacg ccagatgcaa
    24841 aactataaaa gtgaggaagg cagaaaatat gcaggttatg ctcagggaat ttttgataaa
    24901 attgaagctc ttgaaaaaac agtaatagca gcagttaatg gatatgcatt aggtggtgga
    24961 tgtgaacttg ctatgagttg tgatattaga attgcatcag aaaaggctat atttggacaa
    25021 cctgaagtta atttaggagt tattccatgt tttggtggaa ctcaaagatt atcaagactt
    25081 gttggaactg gaatagctaa agagttaatt tttacaggga gacaagtaaa tgcagaagaa
    25141 gctaaatcta ttgggcttat taataaagtg gttccaagtg atttactttt agaagagtct
    25201 atgaagatga tgaaccaaat agttgaaaaa gcgccaatag caataagata tgcaaaagtt
    25261 gttattaaca aagggataga tatggattta aagaatgctc ttgaactaga gaaagatatt
    25321 gcaggtttaa cttttgctac aagagataag caagaaggaa tgaatgcatt tatagaaaag
    25381 agaaagccag tttttgaaaa taagtagcta ttataactaa aattggaaca atgtagaata
    25441 tattcaaaat ctaaatttcg tgaaaaaata catatagttt aattttaaaa ggagttaatt
    25501 tatgaatacg ggagtaatta taagtttagt agggattatc atagctctat ctcttctagt
    25561 tgtaatggtt atgaagggaa taaatatttt tattatagca attgtatgtt catcattagt
    25621 tgctataaca ggaagcctaa atgtgtatga tgcacttaaa gtggattata tgacagggtt
    25681 tgttggattt cttcaggcaa atttctttat tttcctaaca ggtacattaa tgggaaaaat
    25741 aatggagatt actggtggag caaaatctat tgctaagatg attgtaagat ggattgggaa
    25801 ggataaagca ctactttcta ttccaatagc ttgtggaata ttagcctatg gtggagtaag
    25861 tgttttcgtt gtaagttttg cagtgtttcc gatagcttta gaaattttta aagaagctga
    25921 tttgccacga agatttatac cagcagcttt gacttttgga tgctcaactt ttgctatggt
    25981 tgcaccaggg gcaccacaaa ttcagaatat tgttccagca tcaacattag ggacagatat
    26041 tatggctgga gcagtaaatg gttttatatc atgtgcagta atgtttatag ttggaagtac
    26101 aattttgtac aaaatggttt caaaagaaaa agccaatggt ggtcatttta tagcacatga
    26161 atcagatata tttgatgaag cagaagcaac taattctaag agagaaaatg gtcccaatgg
    26221 tgtaattgcc ttaattccat tgatagtgtc aattttaata attaatgtaa aagttaatgg
    26281 aaaagcgata gtaccaattg aggtgggagt atttattgga gctttattag tatatttatt
    26341 gttaaataaa tatcaagata atactaaaat agttggacat gttggtgatg catgtaaaac
    26401 tacagtagta gcaatatgta atacttgtgc tgtagttgca tttggttcag tagtaaaatc
    26461 agcagttggt tttgacttca tagttaatgc catgactaat attcctggtc ctcctattgc
    26521 aggagctgcg gttggaacta cagttattgc aggtatttgt gggtcagctt caggtggact
    26581 aggaattgca gctccacttc ttggaccagt atttttagcc aagggtgttt ccgctgcagc
    26641 attaactagg actatggcaa tttcatcagc agcattagat tcacttccac acaatggata
    26701 tattgttact gtaacaaatg gtctttgtaa agaaacacat aaggatgctt atatgccaat
    26761 attctgggta actgttttaa ctccgtttat tggaaccata gtagcagtcc tactgtttac
    26821 aatgttccca ggattgccat aggagaaaag ataaaaggta taaattatat tttgatatta
    26881 taaaatcaat tttcaaaata tatacaataa atttcatgtt ttaatactgt aaatatgtat
    26941 ttaaaataac aaattgacca tataaggagg aattgaaagt gttaaataaa ccattagagg
    27001 gagttaaggt tattgaccta acgtattttg tagcaggtcc agggacttct aaaatattag
    27061 ctgactgggg tgctgacgtt attaaggtag agccaagctt tggagaccca ggaagaaaga
    27121 caggggcaac aatgacaatg ccaatagatg attataataa tcctttttac agcacatata
    27181 attcaaataa aagaggtttg tctataaatc ttaagagtga aactggaatt gaaattatgg
    27241 ataagctttt aagcgaagca aacgtatttg tttcaagtta tagaacagga gcattaaaaa
    27301 ggctaggtct tgattatgaa tcattaagta aaaagcatcc acaccttatc tggggtcaga
    27361 taaatggttt tggcgatttt ggacctgcta aggataatgc aggatttgat acagttgcct
    27421 tttgggcaag aagtggggct atgcttgata tagcagaaaa agatacaagt ccaataaatc
    27481 cacctatagc ctttggagat gcaacaactt cttgtagttt atctggagga atatgtgcag
    27541 ctttgtacca gcaagctaag actggcaaag gacaaaaagt aatggcatct ttatttgggc
    27601 aagcaatatg gaatgcatca tctttattag catctacaca gtttagtgat gagtatccaa
    27661 aaactagaaa aaatgctatt tcaccagtaa taaattctta tcgttgtaag gatggaaagt
    27721 ggatattctt aagtatttta gagcatgaaa gatattttga ggcactttgt agattatttg
    27781 gaagagaaga cttaataaaa gaagagaaat ttgcatcttc agtagaaagt aagaaaaatg
    27841 ctacagaact tattaatatt ttggatggtg aatttgcaaa gtttactcaa aatgagatgg
    27901 ttgaaaaact tactgctgct gatatagctc atgaaaaaat tcaacatgtt aatgatgtag
    27961 tgactgatga acaggctatt gctaataact atatatatga acatacaagt aaaaatggaa
    28021 ataagacaat gcttgcatcc actccagtta aatttggaaa tattgaagtt aacatgactt
    28081 gtgatgctcc acttatagga gaacattcag atgagatatt gagagaactt ggatacagtg
    28141 attctgatat agaggctcta attgaatctg gaattgtaac aatgaataaa gaagcatgtg
    28201 aaatatgtta ataaacatgt gaagtatgtt aataaatagg agggaatttt atggatttta
    28261 gattaacaga agcacaatta atgcttcaaa gagtagcaaa agaatttgca gagaatgaaa
    28321 tagcaccaat agctgctgaa acagacaaaa caggtatttt tccaagggag ctttttagca
    28381 aaatggctaa aataggattt aatggaattg gaacacctgt agagtatagt ggttctggtg
    28441 gggcagatat agaaaaggta attgttgtta ctgagattgc caaaaaatgt gcagcatctg
    28501 cagcaatatt atcaatacac acaatatatg ctcaagctat tttgaaattt ggtactgagg
    28561 aacaaaagaa aaaatacctt ccaatgatgg ctgaaggtgg gtgtgtaggt gcttttgcct
    28621 taaccgaacc taatgctggt tcagatgctg caagagctgc aacaactgca attattgatg
    28681 aagaaactga tgaatatgtt ttaaacggaa ctaagtgttt tatttctggt ggaggtcaag
    28741 cagaatcatt aataatattt gcactgacag acccatcaaa gggaataaag ggaatgtctg
    28801 ctatcattgt cgacaaaggt actccaggtt tttcaatagg aaaaattgaa gaaaaaatgg
    28861 gtattcatgg ttcagaaact gcagagttaa tatttgataa ctgtagagtt ccaaagtcta
    28921 acttattagg aaaagaagga aaaggattta atatagctat gacttgttta gatggagcaa
    28981 gaattggagt tggagctcaa gctgtaggaa ttgctgaagg ggctttagaa gaaagtatca
    29041 aatattctaa agaaagagtc cagtttggca agccaatttc agctttgcaa ggtattcaat
    29101 ggtatatagc agatatggcg acaatggtgg aatcagcaaa attattagta tattatgctg
    29161 cagatttgaa agctagagga gaaaaacaca ctaaagaagc tgctatggca aaatacaatg
    29221 cgtcacgtac tgcaagagag gttacaaatt tagcccttca aatccatggt ggatatggat
    29281 atatgaagga ttatccatta gagagaatgt acagagatgc caaaataaca gaaatttacg
    29341 aaggtacttc agaaatacac aaagttgtta tttcaagagc agtactagga taggaggaaa
    29401 agaatgaata tagttgtttg cttaaagcaa gttccagata ctaatgaagt taaaataaat
    29461 aaagagacag gtactctaat aagagatggt gtaccaagta taatcaatcc agatgataga
    29521 aatgctttag aagaagctct aaaaatgaaa gatgaactag gagcagtgat taaggttata
    29581 agcatgggac ctcctcaagc taaaagtgct ttaaaagaag ctttggcaat gggagctgat
    29641 gaagcttatt taatctcaga tagagctttt ggtggttcag atacttgggc tacatctaca
    29701 attattgctg ctgctataga gaaggttgga aagtatgatg taatattctg cggaagacaa
    29761 gctatagatg gagatacagc tcaggttgga ccagaggttg cagaattttt agggatacca
    29821 caagttactt atgcgaaaga agtaaaggtt aaagatgaca aattactagt aactcgttat
    29881 acagagacag gggattattt aatagaagca aagatgccag ttttattaac tgctataaaa
    29941 gagttaaaca atccaagata tccaagtgtc aaaggaatct tagaagctta taataatgga
    30001 gatgcaaaga ttactgtatt aacacttgca gatttagatg ttgatacaac tcaaattgga
    30061 ttaaagggtt cacctacaaa cgtttataag tcttttgtac cagttaaaga caagcataat
    30121 gaaataatag aaggtataaa taaaaaagaa aaagctgaga aactaataga aatactattc
    30181 gatttgaaac tagtatagga gtgttaagat gatgagagca aaagttaatc aaggtataaa
    30241 tttaaatgat tataatggtg tttgggtaat aggagagcaa agggaaggta aaattaatcc
    30301 tgttacgata gaacttattg gtgaaggaag aaagcttgct gaccaattag gaaaagagct
    30361 tgcagttgtg atagctggat atgaagtaga gaaagaagtg aaggaattac ttcactatag
    30421 tgttgataaa gtatattaca tcaatgaccc tcttcttaaa gatttcacta ctgatggata
    30481 tgcaatttca attgcaaatt taatcgaaag aaaaaagcca gaggttgtat tggttggtgc
    30541 tacttctata ggaagagata tagcgcctag aattgcaggt aaggttggaa caggtctaac
    30601 tgctgactgt acaaaacttg aaatagattc aacagataat aagttattac aaacaagacc
    30661 agcatttggt ggaaatttaa tggccactat tgtttgtcca aaaaatagac cacagatgtc
    30721 aacagttcgt ccaggagtta tggcaaaagc tgtaagaaac gaatcagaga ctggaatttt
    30781 agaaattgtt actccagaac ttactgaaaa aatgattaga actagattag tagagatact
    30841 tccacaagaa aagaaatctg taaatctaac tgatgctaga ataatagtat cagggggaag
    30901 aggattaaaa agagcagaag gatttgagct tataaaagaa ttggctgata aattgggggc
    30961 agagattggt gcttcaagag cagcagtaga ttcagggtgg atagaacatt ctcatcaagt
    31021 gggacaaaca ggaactactg ttagaccaga actatacatt gcatgtggaa tatctggagc
    31081 aatacaacat ttagcaggta tgagtgattc taaatatata gttgctataa ataaagatgc
    31141 aaaagcccct atttttagta tatgtgacta tggaatagta ggagatttat atgaaattat
    31201 acctgaaatg atagaatcat taaataggta acttttagaa aacattgaat atgtagaatg
    31261 ataagtctta taatattgta ttgtaagact tattattttt gctgaaggtt atagtgggga
    31321 gatgaaacaa tgaaagagca gtggtataag gatatatttg caagagtatt atctatgact
    31381 gatgatggtt ttattgttgt aaatacaagt ggagtaataa tagatattaa tgataaatat
    31441 tgtgactttt taggaaaaga aagaaaagat ataataggac aaaatataca aagtattata
    31501 ccaaatacaa aaatgctaga tgttatgaaa aataagtatt gtgaagaagg agctatacat
    31561 cattattcag gtggaaatac aaaagaaaaa agtgtaatag taagtaggtc ttatgtggaa
    31621 aatgacaatg gagaagtagt tgcaggagta gctcaagtta aatttagatt gcaatcattt
    31681 gatgtagcta aaaagttaat gtcagaatat atggagctcc aatattataa agagcagttt
    31741 aaggataact gtggttttga caagctcata ggtgaaaata gagacttcat agaacttaag
    31801 aaaacaggtg tgaaagcttc taaaacaaac tttccagtac ttttaacggg tgaaacaggt
    31861 acaggaaaag aagtctttgc tagagcaatt cacaataata gcagtaggtc agataaacct
    31921 atggttagta taaactgcgc tgcaatacca gaagaattat tagagtcaga gctatttggc
    31981 tatgacgaag gagcttttac aggagcaaaa aagggtggta aaaaaggtaa gtttttagtt
    32041 gcgaataatg gtacaatctt tttagacgag ataggtgaca tgcctttgac catgcaagca
    32101 aaacttttaa gagttcttca ggaaagtgag atagaaccag tgggaggtct taaaactata
    32161 aagatagatg taagagtaat ctctgccaca agaaagaatt taagtaaaat ggttgaagag
    32221 ggattattta gagaagacct atactataga ttaaatgtaa taaacattca tatgatggaa
    32281 ttgaaggaca ggcaagatga tattttattg ctagcaaatt atatattaaa caaattaaat
    32341 gttgaataca aggaattgaa ggttttaagt gataaggtta aaaattgttt tattaactat
    32401 acttggccgg gaaatataag agaactacaa aatgtaataa aaagtgccta tgctgtaagt
    32461 gatgatatgg tcattatgat gtgtgacctt ccatctaaga tggataatat cagcagagta
    32521 gctcaatgta atgtggatag caattgttca attcatgaaa tggttgagaa ttatgaaaag
    32581 agcttaatta ttgatgtgtt aaggaagtat aattggaaat gttctaaggc agcagaggtt
    32641 atgggaatac acaagagtct tttatataag aaaataaaaa agtatgaaat tgaattaaat
    32701 aattaagata aattgaatta aggcaatgct aaaaaagcgc ctttattcaa agcgaactgt
    32761 ataaactgtt ttatgcagtt aaaacgtgta aaagtagatg tgaagtacta atgtcaatca
    32821 ttagtacttt tttattatat ttataattac tatctatatt tcagtatctt ttgatatcta
    32881 agattattat gtttaattta ggatataaca aaaccctctt agttatggac agttttttat
    32941 attttaactg tctactaaga ggggatagtt tagtttaaat ttagagtaaa ttttatctag
    33001 catttataaa aaatattttg aatagctttt ttgttattta gtcataattt tctacttctt
    33061 tatcaagttc tttgccacaa gaatcacact tatatatagc attgtatccg taatcatatt
    33121 catcaactat tttcttttgt ctaaatacac caccacactc acataggaaa ccaattttta
    33181 taagtgtaac gataaattgt gattttataa attttatatc attacattta tcgttagtgt
    33241 aaaatgtata aatataagag ttatcgcact catctcttaa ttcaaatctt tcaatatatg
    33301 tgttattttt taattcctca aattcttctt tagttaaata tgatttttgt tgcaattcat
    33361 gtaaacgctt aatttggtcc attatttata tcctcccaat aattttagta ttttaagctt
    33421 agataaggaa tctaagtttt attaaattta tcccaaggaa ttatttaaat catatttagt
    33481 tttattaatt acaacatata taaattacac gaaaataaaa ctaattgtaa aaataatatc
    33541 ctgtgaaaac ctaaactact aaccccttac taacccgaaa actaaaaaaa gaaaatatat
    33601 ccaaatataa gtgatatata aataaagatg tttcatataa atttaacttt aaatattctg
    33661 acaataatat atataaatat attat
//
LOCUS       DAEMIK010000026        33629 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEMIK010000026 DAEMIK010000000
VERSION     DAEMIK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.026"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            309..1142
                     /locus_tag="KPK87_001071"
     CDS             309..1142
                     /locus_tag="KPK87_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2537242.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            1268..3109
                     /locus_tag="KPK87_001072"
     CDS             1268..3109
                     /locus_tag="KPK87_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2537243.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            3132..4565
                     /locus_tag="KPK87_001073"
     CDS             3132..4565
                     /locus_tag="KPK87_001073"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2537244.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            5270..6148
                     /locus_tag="KPK87_001074"
     CDS             5270..6148
                     /locus_tag="KPK87_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG2537245.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            6184..8079
                     /locus_tag="KPK87_001075"
     CDS             6184..8079
                     /locus_tag="KPK87_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2537246.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            8347..10254
                     /locus_tag="KPK87_001076"
     CDS             8347..10254
                     /locus_tag="KPK87_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2537247.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSESNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            10244..10693
                     /locus_tag="KPK87_001077"
     CDS             10244..10693
                     /locus_tag="KPK87_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2537248.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            10793..11680
                     /locus_tag="KPK87_001078"
     CDS             10793..11680
                     /locus_tag="KPK87_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2537249.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            12085..13533
                     /locus_tag="KPK87_001079"
     CDS             12085..13533
                     /locus_tag="KPK87_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2537250.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNKIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            13559..15949
                     /locus_tag="KPK87_001080"
     CDS             13559..15949
                     /locus_tag="KPK87_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG2537251.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKIDVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSTLDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            15951..16622
                     /gene="pgmB"
                     /locus_tag="KPK87_001081"
     CDS             15951..16622
                     /gene="pgmB"
                     /locus_tag="KPK87_001081"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG2537252.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLA"
     gene            16661..18061
                     /locus_tag="KPK87_001082"
     CDS             16661..18061
                     /locus_tag="KPK87_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2537253.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGIPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYIFG
                     VEKNVKEGIDNVVEEV"
     gene            18376..19219
                     /locus_tag="KPK87_001083"
                     /pseudo
     CDS             18376..19219
                     /locus_tag="KPK87_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            19364..21292
                     /locus_tag="KPK87_001084"
     CDS             19364..21292
                     /locus_tag="KPK87_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2537254.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIV
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            21353..22780
                     /gene="ascB"
                     /locus_tag="KPK87_001085"
     CDS             21353..22780
                     /gene="ascB"
                     /locus_tag="KPK87_001085"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2537255.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            23191..23967
                     /locus_tag="KPK87_001086"
     CDS             23191..23967
                     /locus_tag="KPK87_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2537256.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            23983..24348
                     /locus_tag="KPK87_001087"
     CDS             23983..24348
                     /locus_tag="KPK87_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2537257.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(24514..25263)
                     /locus_tag="KPK87_001088"
     CDS             complement(24514..25263)
                     /locus_tag="KPK87_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2537258.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            25741..25875
                     /locus_tag="KPK87_001089"
     CDS             25741..25875
                     /locus_tag="KPK87_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537259.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     "
     gene            26389..26919
                     /locus_tag="KPK87_001090"
     CDS             26389..26919
                     /locus_tag="KPK87_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537260.1"
                     /translation="MEKILENTLLLMILPWIFVWGILCILEKRKRKNEDGNKDEKSYV
                     IQNDNIFFPFFSLGMFLFSFCLFYTVFFTEFDISEVISVSVFVIFFAILTLDSAIWRV
                     IVDGDIIKYRSFYGITRTYNFNDITKGVYTKKGSLKIYIEGKRIFTFYDNLEFSLFEN
                     QMRKRKIPLEYKRKEK"
     gene            27204..27482
                     /locus_tag="KPK87_001091"
     CDS             27204..27482
                     /locus_tag="KPK87_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537261.1"
                     /translation="MKKLEFIFYGGESLIIKFLEKKLTISGKLARVLGTILMVLIGTF
                     LLYCAINPPTYFHFLKENINRIIYGVIGVLLILKALRMLFTNKLKNKS"
     gene            27621..28835
                     /locus_tag="KPK87_001092"
     CDS             27621..28835
                     /locus_tag="KPK87_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2537262.1"
                     /translation="MNDKNFIEELRQKREDYGVTQTRLAIACGISREYYNRIEKGKQS
                     LNDELKEIIEKQIERFNPQETLFLLIDYFRVRFPTTDALKIIRDVLQLKADYMLYEDF
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERTWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKAKYKAGECISYFRMQKDYSGTEKCGSDTPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEVEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWKMNDDWAWFVGDNREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDKENHTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTVVPQENDGIF"
     gene            29494..29703
                     /locus_tag="KPK87_001093"
     CDS             29494..29703
                     /locus_tag="KPK87_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBG2537263.1"
                     /translation="MNCLERFYRKGVCVIDNTVKTIQKKNNKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            29815..31428
                     /locus_tag="KPK87_001094"
     CDS             29815..31428
                     /locus_tag="KPK87_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2537264.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDEETKWIVEKIFSLAYQGYGSAKITKILREEKVPTSSWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYSCSFYGQFGKGYCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LHKIQKVGNAERIREKKKKANALKKAENRQNEIDRLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            31457..31780
                     /locus_tag="KPK87_001095"
                     /pseudo
     CDS             31457..31780
                     /locus_tag="KPK87_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            31812..32999
                     /locus_tag="KPK87_001096"
     CDS             31812..32999
                     /locus_tag="KPK87_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG2537265.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGIPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            33191..33595
                     /locus_tag="KPK87_001097"
     CDS             33191..33595
                     /locus_tag="KPK87_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG2537266.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT        13151 a         3722 c         6169 g        10587 t
ORIGIN      
        1 atatatgacc ttaattttgt tatgaaataa cttcaaaact tattttagca tgtttagatt
       61 atataaatca tataaaaaaa gtagaatgag tgaatactta tgacaataat ctagtagaga
      121 tatacatgaa attatatatt gactaagtac agttattatt ttataatgaa aataaagtta
      181 tcatacagat aaataaacgg atcgttactg agatagatgt aatcaggcgt gacctaaaaa
      241 ttctagtaaa tgcttaaagt attattagtc ttttttaggt ctctttttgt ttacagtgga
      301 ggtagtttat ggttataaag aaagtattta ataataatgt tgtatttgcc agagataatt
      361 caggacaaga agtaatatta atgggaaaag gaattggatt taataaattt actaaagatt
      421 tagttgaaat agataaaatt gaaaagaaat ttatattcaa taatgaggaa tcattaagtg
      481 aattacattc actactagat aggatacctt taaaagatgt tgagcttgta agtgaaatta
      541 ttcaaatggg aagagaagaa ttgggtgata atcttaatga tagtatatta atcacattat
      601 cagaacatat aagttatatg ttaaaaagaa tggatgaagg attagtattt caaagcccgc
      661 ttcaatggga aataagacaa atatatccta atgaatataa ttttagtaag aaagctgtag
      721 aatatttaag aaaaaaatta gataaaaata ttcctgattc tgaaatagca tttattacga
      781 tacactttgc aaatgctcag ttagaaacaa aagatatgca agaaacttta ttgttaacta
      841 agataattga taatgtatta gatatcataa agtatcacta tggaatcgaa ataaatgaat
      901 attcctttga ctacacaagg tttataagtc atttaagatg ttttataaaa aggcaattaa
      961 gtggtgagaa tacaaatgta gacacatcat tgttagatat aataaaaatg aaatataaaa
     1021 gtgattacaa ttgtgcaaaa aaagtaaaaa actttcttga aaagacgtat aaatggaaaa
     1081 ttagcgaaaa tgaattgttg tacttaacat tgcatttaaa tagattatca aatataaagt
     1141 aaatagtgta ggattgttac tgtttggcag gcatgaccta agttggtata attagttttt
     1201 aatatctaat tgtattgatt taggtctatt tttatacata aaattatgaa aatggaggat
     1261 agattgtatg aaatttgaaa gagagtctga agaaattata aaatatgtcg gaggaaaaga
     1321 aaatattatt agtttagttc attgtgcaac taggctacgt tttaagctga aagatacttc
     1381 aaaaactaac aaagaagagt tattaaaaat gaaggatgta ctatctgtag taaatagtgg
     1441 aggacaatac caagtggtaa ttggaaataa agtaactgat tactttgaaa ctattataaa
     1501 aaaagcagtt ttacaaggac aaaatactga agaacaggga gaaaaagttt caatagtttc
     1561 taaaatattt gaatcaattt caggtgcatt ttctccatta atacctgcac tagctggagc
     1621 aggaatgatt aaggcactgc ttacaatact aactactatg aaatggatgt ctgatacaag
     1681 tagcacttat gctatattat cagcagctgg aaatgctgta ttttactttt taccaatatt
     1741 cttaggtatt accttagcta taaaacttag tgcaaatcca tatgttggtg gagtgatagg
     1801 agcagctttg ttagatccta attttacagc tttaattgga gcaaaaggtg gtgctaactt
     1861 ttttgggatg ccagtaacag caattgatta ttcttttggt gtattcccta tatttgttgc
     1921 tatatttatt tattactatg ttgataagct tttaaagaag attatattaa aggatttaca
     1981 attattctta gtaccgatga tatcattatt agtcatggtt cctttaactg tgattttatt
     2041 tggaccaata gcaactaatg ttggtgatat aatatctaat atttttatgg gattatttga
     2101 atttaacaga gtaattgcag gagctgcaat aggtatagct atgccattct tagcagtatt
     2161 aggactacac tggggcttca caccaattac tttgcaaaac ttggctacaa tgggtggaga
     2221 cccaatagaa ggtgcagctg ttgcagcagt atttgctcaa ataggtattg caataggttt
     2281 atttatgaag gttagaaaat ctaaaaaaaa ttcagaatta aaggtattag caggttcaac
     2341 agcagttaca ggaattttag caggagtaac agaacctata atttatggaa ttatattaag
     2401 atacagaaga atgattccag tattggctat tgcaggaggt attggtggtg gtatatgtgg
     2461 tgtatttggt gttacttgta atgcatatgt atttcataat atattctcaa tgccagttta
     2521 tacaccattc ctaggatata tattaggaat aggaacagca ttagtgctag gagcagtatt
     2581 gtcatatttc tttggattga gtaaagaaga atgtgaagaa ggtaatgaag aagttgcttt
     2641 tgatgaagat gatggtatag aggagatatt atctccaata gaaggcttac aaatagagtt
     2701 atctgaagta gaagatgaag tgtttgcatc aggaactata ggtaatggca tggcaataat
     2761 accttcaaag ggagaagttg tgtctcctgt aaatggaaca ataacaacta tttttcctac
     2821 aaagcatgca ataggaatta cctctgataa aggtgtagaa atattaatac acgtaggtct
     2881 taacactact atgttagatg gaaagtatta tactactcat atgaatgcag gagataaagt
     2941 taaaaaagga gataggattg tttctttcga taaggataaa atagaaggtg aagggtatag
     3001 tattgttaca ccaataatac taactaacac aaatgattta gcagatgtaa tagttaaaga
     3061 aaataaacat attaaaattg gaacgcctgt aataaaagtt gtttattaat tatactggag
     3121 gagatatttc tatgaaatct aatcaagaat ttttatgggg aggggcagtt gctgcccatc
     3181 aagttgaagg tggatataat aaaggtggaa aaggaataag tattgcagat gtgatgactg
     3241 caggtactca tactatttca agaaagataa cagatggagt aattgagggt ttaaattatc
     3301 ctaatcatga agccattaat ttttatgaaa attataaaga agatattaga ttatttgctg
     3361 aaatgggata taagtgtttt aggacaagta ttgcttggac tagaattttt ccaaagggcg
     3421 atgaatcaac tccaaatgaa gatggattga aattttatga tgatttattt gatgaattat
     3481 taaaatacaa tatagagcca gtaataactt taagtcattt tgaaatgcct tatcatttag
     3541 taaaaaacta tggtggatgg agaaatagaa aattaataga tttctttgta aacttctgtg
     3601 aagtggtaat gaatagatac aaggataaag ttaagtactg gatgactttc aatgaaataa
     3661 ataatcaaag tataactaca aatcctattt atgcatttac taactcggga ataatctatg
     3721 aagagcagga agacaaagaa gaagttatgt atcaagcagt tcattatgaa tttgtagcaa
     3781 gtgcaaaggt tgtgaagata ggtcatgaaa taaaccctga gtttaaaatt ggatgtatgg
     3841 tggcagctat gccaagttat ccttattctt gcaatccaga ggatatgatt aaatttgtag
     3901 aatctaatag agaacaactt atgtttactg atgtacatgt aagaggtcat tatccaagac
     3961 atacactaaa attatgggaa agaaaaaatt ataatttaga tattacagag gaagataaaa
     4021 aaatcctaaa agaaggaata gttgatttta ttggatgtag ttattattta actactgtag
     4081 taacagctga taaaactatg aaaacaacag gtaatgactc ggcaggaaag gctgatgtag
     4141 ttgaaaatcc atacttaaaa atttctgatt ggggatggaa tatagaccct gtaggtctaa
     4201 ggttttacct taatcaacta tatgataagt atgaacttcc tatattcatt gttgaaaatg
     4261 gatttggagc agaagatgta ttaaaatcag ataatactgt agatgatgat tatagaatag
     4321 agtatttggc tagtcatata agggaaatga aaaatgccat tgaaattgat ggtgttgatg
     4381 ttatagggta tactgtttgg ggatgtatag atccagtatc atttacaaca ggagagatga
     4441 aaaaaagata tggatttata tatgtagata aaaataatga tggaagtggg actcttaaaa
     4501 gatataaaaa gaaatctttt gattggtata aaaatgtaat aaaatttaat ggagaaattt
     4561 tataggtaga taaattttaa tatttataag attatgttaa ggaaaccatt aaaaattaga
     4621 ttaaaatcgt gggtatttaa atccactaaa agatattgac tatttagtca atatcttttt
     4681 ttattaaaaa gtaaatataa agaggtaagg ttaaaaattg ggatatatat gatttatata
     4741 gtatgattta attagaagga gctatattag tattcatttg tatagttgga gaataaatgt
     4801 cagtcttacc ttacgatttt tataatagtc ctatttaatt tctattgtaa ctaatatgca
     4861 agaatttaag atagaagtat ctatgactta tggtgtatta ataattggta tctgctatct
     4921 agtatctttt taattttttt gattccaata tagtgctaac tacgatacga atatctttaa
     4981 atttatagct aaactatcta attgtttatt ggaaagagtt aagaaggcta aagcataaaa
     5041 cacttaaaaa gagaagatgt atatgagaaa tataattaac tatgatgcag acttctaaaa
     5101 attatttagt gaaaaggaac ttagagtgta taataaaagt tccttttcac taaggaaaac
     5161 gattgttatt ttctgatctt cattaccctg atattattta aatataaaca acaacaaaat
     5221 taacaagaag caaattcaac ctaaaattta aaatatcggg aggtacaata tgttaataaa
     5281 tatgaaggaa atgctaaaag tggcacaaga aaatcaattt gctgtaccag catttaatat
     5341 aggaagtggg caaatactta aagcagtagt acagagtgct aacgaaaaaa atgcaccagt
     5401 aatacttgca atacacccaa atgagctaag ttttctagga gatagctttg tagctagttg
     5461 catagaagaa gcaaataaga gtaaagtacc tatggtaata cacttagatc atggggaaaa
     5521 taaagaacaa atattaaggg caataagatg tggatttaca tcagtaatga ttgatggctc
     5581 acatttacca tatgaagaga atgtagctat atctagggaa atagtagaaa tagcaaaagg
     5641 attaaatgtg tccgtagaag gagaacttgg aacaatagga acaacaggaa catcaagtga
     5701 aggtggtact gatgaaataa tatatacaga ttcaaaatta gctaaggatt ttgttgaaaa
     5761 aacaggagta gacacactag ctattgcaat tggtacagca catggtatat atccaaaagg
     5821 ttttaaacca gagcttaaac tagatttatt aaaagaaata agagaagtag tagatatacc
     5881 tttagttctt catggtggtt catcaaatcc agatgaagaa atagcacaag cagttaaact
     5941 aggagtttgt aaaataaata tttcgagtga tgttaagtcg gcatattata agaagtgcag
     6001 agaactatta gaacaaaacc caagcttata tgagccagat actatatatc caccatgtat
     6061 aaaatctgca agagaagtta tagagtttaa aatgaatcta ttcaatgcta tagataagtt
     6121 aaagtgcttt tataaaaata aaaattaatt atgaggtaac aaattaaaag gaggggagaa
     6181 tttatgatag gtaaaatact acgaagcgat tgtattgtac tagataaaca attacaaaca
     6241 aaaaaagatg taattaatta tatggcaaat catttgtacc atggaaatta tataaataat
     6301 aaagaaaagt ttatagctga tattaataaa cgcgaaaaag aatcatcaac taatatgggt
     6361 aatttaatag ctatacctca tgctaagtca aatgtagtaa atcaacctgt aatgatgtat
     6421 ttaagaacag atagagagat tacagataat gatggtcaaa aacttaggtc attttttatg
     6481 atagcagttc cagaatcagg ttcagatgaa catcttaaga ttatatctaa actagctaca
     6541 tatctaatgg aagaaaattt tcttaagcat ctactaagtg cagaaactcc atacgaaata
     6601 ttaagtatat ttaataataa agctgcagaa tcaggagatt ttagtaataa tcaaaataaa
     6661 ggtcttatag ttgggataac aggatgtcca acaggtatag ctcatacttt tatggcaaaa
     6721 aaagcattag aggaagcagg gcaagaactt ggatatgaag ttaaaataga aactcatgga
     6781 gctataggtg tagaaaataa gttgactaaa gaagatataa aaagagctga atacgtaata
     6841 atagcttgtg ataaagaagt tcaactgagt agatttatag gaaagaaagt gctacaaaag
     6901 aaagtagtag atgctaagaa tgtttcatta gctaaaaatt taattataaa cgctgaaaat
     6961 ggaatggcta aagaaattaa accaggtgga aagactttag aagattcaaa agaaatgctt
     7021 caagggtcag agaaaaaggg tgtttataag cacttaatga gtggtgtatc tgaaatgatt
     7081 ccatttgtag tgataggtgg aattggtata gccatagctt ttatgtttgg tatatttgca
     7141 tcagacccaa ctcatgaaac atacaaccct atagctggat tctttggaca acttggaggc
     7201 gattcagcat ttaagttatt tataccgata cttgctggtt ttatagctaa atcaatagca
     7261 ggaagacaag gatttgctcc tgctatgata gctggtttta tggcagttat tggaggttca
     7321 gggtttatag gtggtatggt agctggtttt gcaacaggat atatagcttt aggagtaatg
     7381 aagataacag aaaatttacc agatgtatta aaaggagtaa atgctgtatt gatatgtcca
     7441 cttttgattg catttacgag tggagctata atgttctttg tagttaatac gccagcatca
     7501 tggttaaatg aagctttaca atcttggtta caaagtatgt ctggaactaa taaagtatta
     7561 ttaggtgcaa tattagcagg gatgatggca tcagatatgg gaggacctat taataaaaca
     7621 gcttcagctt ttggattggc tatgttttca agtaatatat ttgaaccatc agcagcatta
     7681 atggtaggag gaatggtacc tccattagca atatcattgg ctatgttaat attcaaaaat
     7741 aagtttacta aagaagaaag aaagtcatct ctaccaactg ctataatggg ggcatctttc
     7801 ataacagaag gcgcaatacc gtttgcagct gcagacccat taagaatgat tccagcaaat
     7861 ataattggtt cttgtatagg tggggcaata tgtatgagtt tagatataac tttaatggca
     7921 ccacatgggg gaatatttgt aataccattt gcatgtagtg caccagttgc atatatagct
     7981 tgtatattga ttggtacagt aataacagca ttgattatag gggtaacaaa gcctactata
     8041 aggagtgaaa atagtgaagt tgaattactt ggtgcttaaa ttataaataa tagtaaaaat
     8101 aattaatgga ataacaacaa gtatatagaa taataataag gatatttaga atcagttaac
     8161 tacttctaaa tatccttaat ttttattttg aaattatatt atctattata tcaatttagt
     8221 aaggtgtatc ataaattctt ttatgataca gaagtatctt agtaatttta catagttagt
     8281 aaatatgtta aaatagtact taatataaac atagaaatat ataaacatag gtagggaggg
     8341 ctaagtatgc ttgtgtcgag gcaaattgaa tttcttaaat tgatactaaa cgaaaataaa
     8401 tatagaccta taaaatactt taaagataaa ttaaatgtat ctgataaaac tttacaaaaa
     8461 gatttgaaga tgattgagaa atatttagaa acttttaata taaaaattga tataaaaaga
     8521 ggctatggaa tattaataga agatttagca aagaagaaca tagacttaat taatagttta
     8581 aatatacaat cgaaggaaaa taaatcttta tcagtagagc agagaagaat ggagatttta
     8641 aagtatttat tactaaattc aaataatata acttcaataa atcagttagc agataaatat
     8701 tatgtaagta aaacttctat agtaaatgat tttaaatata tagaggcatg gataaaagaa
     8761 tacaatttaa aacttaataa aactttagaa ggaactaaga ttataggtaa agaagtggat
     8821 ataaggaaat cgattgccaa aatgatggat gatttattag atgaaaatta tgaacaacat
     8881 gatatatctg agttaacaag attagattct gctacattta gtgcattgat aaatttattt
     8941 gatattgatt ctattatatt tgtagaaact ataattacag aattagaata caatttagga
     9001 tatactataa atcagcccta ctatataaat ttaattaccc atatacttat atgtttaaag
     9061 agaatcgaag aaggtaatca aatagaatct caggaagaaa gagaaatttc tgtagataat
     9121 ttggacgagt tagtatataa aaatgtcact acacttatag ataaaataga aaatagatac
     9181 agagtaaaaa taacgaaaga tgaacttatg tacatatata tttttttggt atcatctgga
     9241 tttagtagcg aaagtaatag tagttacaat gatgaatcag atagattaag tgaaagtgaa
     9301 aaagtttcat ctataatgat agagaatatg tcacgatttt taaatataaa tctcaaaaat
     9361 gatgacttac tacagaaaag tctagcatct catgtaaggc ctatgttaaa cagattaaga
     9421 tatgatatcc aaataaaaaa cccgttgtta ggagagatac aagagcgatt ttcagatgtg
     9481 ctgggattat gtatgatgac tataaatatt atgacagagt atgataattt gaagaatatt
     9541 agtatagatg aagtctcgta tcttgccaca tattttcaag cggcaataga aagaaatatg
     9601 agtagtaaaa gagtaatagt agtttgtcat acaggatatg gaacatctca gctccttgca
     9661 gccagattaa aaagagaatt tcctgaatgg actatagttg atatagttcc tatgcatcag
     9721 ttgagtaagc gaaatttaga tgatgtagac tttgtaatat ctactgtaaa cttagatttg
     9781 aaagataaat tacatatagt tgtatcagta ctattaatgg aaaatgatat taataatata
     9841 aaaaatgctt tactttctaa acctaaaaaa agtatgaact tagatacaaa cttattagga
     9901 atgtatttag aagataatat atattttaat tttgatgtag ctcagatgga aagtttaata
     9961 ggtgtgaatt tagggagtaa atatgaatct atttcattag gaaaagattt aaatatatat
    10021 atacataagc aaagtaaatt aaataaaggg ataatgaata tcaataatgt tactagaacg
    10081 attgatattt atttagaaat tagtaatcaa agttttttaa agaatatttt aatggaaata
    10141 tcaaatttat ataggcaaaa gaactatata aactatataa tagattgcaa gagcaaagag
    10201 gatattaaaa tattatttaa gaataatcta ttggagaaaa caaatggaaa ttagagaaat
    10261 attaacagaa gaaagcatta ttttagattt gaaatcaact acaaaagaag gcataataca
    10321 ggaattagta gataaattat acgatacagg aagtataact ttaaaaagta agtttataaa
    10381 ggcattaaaa catagagaaa aaataggctc tactagtata ggttatcaaa tggccatgcc
    10441 acatggaaaa acaaaatatg taaagaaggc ctctgtagta ataggtaaat ctaaaaaagg
    10501 tgtagattgg ccaagtatag atggcttacc tgtaaaagta ataattatgt ttgctatgcc
    10561 acaatctgga ggcaatgaag atatgatatt aatatctaaa atagcttaca ttttaggtgg
    10621 taaaaatata ggtgaaagac ttttaaatgc acatacaaaa aaagaaatat tagatatatt
    10681 ttatgaagtg taggtattgt ataaattata aatttgaggt ttttaaaata aaatgtgtat
    10741 tcttttaaat agtatagatt aatttttaga cactagagag gagaggatat atatggcaat
    10801 atatttagaa atgcctaaat taatacatga atttcctttt cgtagtctat caaatgaagg
    10861 agaagtatta acaacaccac actggcataa ggaaatagaa atattactta taacagaagg
    10921 tgtagtaaat ctatttataa atgacaagcc tatgcaatta aaaaaaggcg agatagtgat
    10981 aataaaagga ggagatattc attatatact gccatcaccg ggaagtgaaa gattagtatt
    11041 tcaatttgat atatcttttt ttaatgattt aattttatta aatgaggaaa aagaaagctt
    11101 aattaattta ttcagtagta taaaaaaatg tagtagagat tggtcagcaa aaataagaaa
    11161 gtcagttttc gatatattga tagatatcta caatgaagac ctatacaaaa tagaaggata
    11221 tagttatgca ataaaaggta aaatgtataa tttaattcct atattatata gacagattcc
    11281 tagagaaatt gaagaaaata cagtagaata tgaaataaac tctaaagaga tattagagag
    11341 attgaacaat atattcaaat atgtagaaaa aaattataaa caacctataa aattagaaga
    11401 tgtgtcttat gaagtaggat ttagtgtata ttattttact aaatttttca aaaaaaatac
    11461 aggaaaaaca tttataactt ttttaaatga atatagatta gaaaaagcaa agtggatatt
    11521 acttaataac aatatatcca ttgtagattt ggttgaagaa gttggttttg gaagtacaaa
    11581 aactttttac agagtattta aagaaaaaat tggagtctct ccattagaat ttaaaaaaag
    11641 atataatagc ttggagaaaa aagggagtgt ctcaaaatga acttagttca tgaggcactt
    11701 tcttttatat gaaatcaaat atattttcag tatttcaaca atgaaaattc cattcattta
    11761 taaagtctaa attttagttc tcaatacaac ctctatttcg aaataaatcc ttttatatat
    11821 ataaatatac agcaatatat gagaataaga taacaataat gaggatataa aaatgtattt
    11881 gctatatata taattatata catagatatc taatattgat atttaatatg aaagtattga
    11941 aaatagacaa tattaattat agtgagtaaa aaatataatt ggatttttaa attgaattat
    12001 ttaaaaggta tagtatgaaa aaaatcttat aactaaatat gagatagaaa ttataaaact
    12061 ttatattgtg aaagaggtag atgcatgaaa ttagggaaag attttttatg gggaggggca
    12121 atttcagcca atcaaagtga aggattttat ttagatgggg gaaaaggttt atctcagttt
    12181 gataaactac cattaaatga taagagatta aaagatgtat gtttagatga ggataatatt
    12241 ttaaatgaaa ataatcaata ctatccaagt catataggga ttaaattttg tgaaaattac
    12301 aaagaagata tagcaatgtt agcagaaata ggttttaaat gttttagatt ttctatatct
    12361 tggtcaagaa tattcccaac aggagaagaa agtgaaccaa atgaaaaggg attacaactt
    12421 tatgataata taataaaaga attaaaaaaa tataatatag agccattaat aacgataagc
    12481 cactttgata taccttattc tctagttgaa aaatattctg ggtgggctga tagaagagtg
    12541 gttaatttat atgtaaaata tgctgaaatt ttgatgaaaa gatataaaga tgatgtcaaa
    12601 tactggattc cttttaacga aatgaatatg ataatgcata ttccatatat aggtgctggg
    12661 ttaactttta tagctgatga aaatcaactt cagaaaaaat atcaagcagc acaccatcaa
    12721 ttattagcta atgcaaaaac tatagaaata ggaaggtata taaataaaga atttaagttt
    12781 gggtgtatgt tagctgctgg aaaaacatat ccatacacat gtaaaccaga agatgtattt
    12841 gcaactttag agcatgaaag gcataatttg tttttttctg atgttcaagt taggggtgtg
    12901 tatccaagct ttatgagtta ttatttaaaa tcaaataaaa taaaattaat aataaaagag
    12961 gaagatttat caacacttaa ggataatact gtagattttg tatcttttag ctattattct
    13021 agtgcttgta gttcagctag agctgaaggt ttagaaaaat caaaggcaaa cggaccagaa
    13081 accataaaaa atccatatct tccaaaatca aatagtgtat ggcaaaacga ccctgtaggg
    13141 ttaagaataa ctatgaatca gttatatgaa aggtatcagt tacctttatt tatagtagaa
    13201 aatggactgg gtactcaaga tattgtaaat gaagatggaa aaataattga tgattatcgt
    13261 atagattaca tgaaagaaca tattaaaaat atgattcttg gagtaaatga agatggaata
    13321 gagcttatag gatatcttat gtggggatgt atagatgtaa ccagtgtaag tgaaggaaag
    13381 atgacaaaac gatatggcat aattcatgtt gattctgatg actatggaag aggctcattt
    13441 aaaagaacta aaaaacaaag tgctttttgg tataaaaaag taatagaaac agatggagaa
    13501 attttattta atgatatgga aactcatgca taattcattt taagaaagga gtgttattat
    13561 gagtttaata tttactacta aggaccaact tatagagcaa gggtttagta aggatacaat
    13621 aaaatccaat gaaacgctgt ttacactagc taatggacat ttaggagtta gagggaatat
    13681 tgaagagtct gattttaata atgagtatgt agataatatg ggtacatatg taaatggatt
    13741 ttatgaagaa tctccaatta catatggaga aagcgcttat ggctatgcaa aacaaaatca
    13801 gactatctgt aagttaccta atgctcatat agtaaatttc tctattgaag gagattattt
    13861 tgatttatca aaaggtatta caagtgaaca tactaggata ctagatttaa atgaagggat
    13921 tcttaaacgt tcatttatat gggaaaactc ttgtggtaga aaagttaaag tatctataga
    13981 aagattagta tcacaagata tagaagaatt aatgatgatt tcatatacaa taacaccatt
    14041 gaattttaat ggaaaaataa aatttaaaaa taaaatggaa aatagtatgg agaagtttaa
    14101 ttgtgaaaaa gtagatgacc caagagtggc atctataaat aagaagcaat ttaaaattga
    14161 tgtaaaaaaa tataacaata agtattttat gaatttagtt acagaaagaa gtagattagg
    14221 gttatggtgt gggtttgatt catatattac ttgtggaaat attacaaatg agacctataa
    14281 tgtttcagat gaatgtattg agcgagaaat agaagtctat gccaaaaaag atgaaaggtt
    14341 aaatttagaa attgttgtag gctatagtag agtatattct gatttaaatc taaatgaaga
    14401 acgtttaaaa tcattagaat gtctggagtc gttaaaatgt ttagaatcag aagcacattt
    14461 aaaatcatta gaattggttt taagagaagc atgtaatatt ggatttgata aagtcaaaaa
    14521 tattcagaga aagaaaatga atgagttttg gacaacctct gatgtagaga ttgaaggcga
    14581 taaaactctt caactgggga taaaaaccaa tttatttcat atttatcaat cagcaggaag
    14641 agatggaaag actaatatat cagcaaaagg tcttacaggt gatggctatg aggggcatta
    14701 tttctgggat acagaaatgt atatattacc attttttata tatactcaac catccattgc
    14761 tagagcttta ttagattata gatactcaac cttagatgca tcaagagaaa gagctagaga
    14821 acttggaata aataaaggtt gcttatattc atggagaact ataaatggta atgaatgcag
    14881 tgcatattat ccagcaggaa cagcacagtt tcatataaat gcagatatag cttatggtat
    14941 tagaacgtat tttgaagcaa ctaatgatga agaatttatg atgcaaaatg gcttagaaat
    15001 tcttatagaa acagcaagat tttgggttga atttggagat tatatagaat caaaaaataa
    15061 taaattctgt ataaatggag taacaggacc agatgaatat acagcaatag taaacaataa
    15121 ttactacact aatttaatgg ctaagtataa tttaatatat gcagtacaga tggtagataa
    15181 atttaagcat aatttatttt caaatgaagt atttagaaaa gttaaattac aagaatatga
    15241 agtgaaaaac tggataaatg ccgctcaaaa tatgtattta ccatatgatg aagagaagaa
    15301 aataagcaaa caagacgatt catttttaga aaaatcagta tgggatttta aaaatacacc
    15361 aaaagaaaaa tatccattac ttctccatta tcatccatta acaatatata gatatcaagt
    15421 aaataaacaa gcagatactg tactttcagc gttgcttttc cctgatgagt ttagcttaga
    15481 acaaaagaag agagattttg aatactatga aaaaataact actcatgatt catctttgtc
    15541 aaggtcaata tttggaatga tggcaagtga aattggaaat catgaaaaag cttataacta
    15601 ttttatggac acagcattaa tggatattaa taacctacaa aaaaatacaa gtgatggaat
    15661 acatgctgct aatatgggtg gaacatggat gagtttagta tatggatttg caggaatgaa
    15721 aataaaaaat gataaattaa gttttgaacc aaggatacct aaacactgga aaaatattaa
    15781 atttaatata ttatttagag ataatttagt ttctgtaaat atagaagaat ataaatctta
    15841 ttataagtta atcaaaggta gaaatataac catattacat gataataaag aaataaaatt
    15901 atcaaagaac ataatagaag ttaataattg ttttaaaggg agtaattaat atgaaagcag
    15961 tcttatttga tcttgatgga gttataacag atactgctaa gtatcatttt gaagcttggc
    16021 atgagatagc aaaatcgcta gatatagaaa tagatgaaac ttttaatgaa aaattaaaag
    16081 gcataagcag ggaagaatca ttagataaaa tattgttatc tgctaataaa gtagatagtt
    16141 atactaaaga ggaaaagtta aaggtgtgtg aagaaaaaaa taacatttat ataaagttaa
    16201 taaactcatt aacatataaa gatatattac cagggataaa tgagcttata aaagaactta
    16261 aaaaaaataa tgtgaaattg ggaatagcat ctgctagtaa gaatgcacct aggatattag
    16321 aatctttggg aataaaagat gaatttgatt gtatagtaaa tccatcctta ttagaaaggg
    16381 gaaaaccata tcctgatatt tttattgaag ggagcaagat gctaaattta aaacaatatg
    16441 attgtgtagg aatagaagat tcttattcag gtattaaagc tataaatgct gccaatatga
    16501 catcaattgg tgtggggaaa aaagaagtac taggaaaatg tgaccatatt gtagaagata
    16561 ctagcttatt aaattacaat acactattac aagcttggag taaaaagatt aatttggctt
    16621 aaaactagat taaaaattat tttaaaagag aggataaggt atgaagaata aatatgaaga
    16681 tgtagctcaa ttgataattg atggacttgg aggaaaggat aatataattg acttaactca
    16741 ttgtatgaca cgattaaggt tcatcttaaa agatgaaaag aaagtcaatg cagataaatt
    16801 aaaatctata gataaggtag ttggagtaaa ctcaacttca actcaatatc aggtcataat
    16861 aggtaatgag gtaggtgttg tatttaatgc aataataaat aaggggataa ataccacagg
    16921 agatattaaa agcgatataa aaaataaaaa taaaaaaaat agtattttaa ataatataat
    16981 agatacaata actggttgta tgacacctat gataccagcc cttacagcat ctgggatgat
    17041 aaaagttata ttatctcttt gcaccacttt taattggcta tcatcaaatt caagtacata
    17101 tagagtatta gatataatag gagatgtagc attttacttt atgcctataa tacttgcaat
    17161 aaatgcagct aaaaaattta atgttaatac atcaatagca gttatagtag taggtgtgtt
    17221 tttacatcca aatttctctg catgggtatc aagtggggac cctatatcat ttataggaat
    17281 accaattcaa ggtgttatat atgcagcatc tgttatccct gcacttttaa cagtatggat
    17341 gatgtcatat atagaaaaat tcattgataa attaacacct agcatgttaa aaacaatttt
    17401 aaatccaact ttagtattat taataagtgc gccaatagca ttaatagtaa ttggaccaat
    17461 aggaaatttc ttaggagagg gattagctag tataataaat ttactacaag gaaggttagg
    17521 atttataatg gtttgtttat tagcagctgc aatgccattt atcgttatga cagggatgca
    17581 tcatgcattg actcctatat tcatatcagc atttgcaact acaggacaag aatcgctaat
    17641 attaatatct caagtttgtg ctaatttagc gcaaggtggc gcgacattag ctgtagcatt
    17701 aagaagcaaa gatagtaaga tgaaacagtt agcatcagct gcatggatat cagctattat
    17761 gggtataact gaaccagctc tttatggagt tacattgaaa cttaaaaaac ctgctatgtt
    17821 agcatctata ggagcaggaa tcgcagggtt atttgcagga attactcatg tgactttata
    17881 tgttccacaa aatagtataa tggcagtact tggattatca ggggaaaagg ggatttcaaa
    17941 tatagtaaat ggacttatag ttatatttat agcattggtt gctccattta ttatgacata
    18001 tatttttgga gttgaaaaaa atgttaagga aggtatagat aatgtagtag aggaagtata
    18061 atattaacta tttacattaa tagaattttt attattattg actataactt gttttgtatt
    18121 tatattgatg caaagaaata agttaatatt aataagaata attattagaa ctgacaaaaa
    18181 gcaacataaa agattgatga agtatggaaa taaaaagttg acaaaatatg tctgatgttt
    18241 tatcatatta attggaatta ggtagtaatt gtttaagtac aagctaagct tattcattaa
    18301 taatagcagt atcctaggaa ggagtatgcc cattattatg gataagcttt ttttatgctt
    18361 aaaaggagga taatcatgat aattaataag atattaaaca ataatgttgt aatcacatta
    18421 gatgataatg atgaagaagt tatcgttatg ggtaaaggaa ttggttatca aaaatcaaaa
    18481 ggcaatctaa tagataaaac taaagtaaat aaagtattta gaatatcaaa taaagaaata
    18541 tcaaacaaac tacaagaatt actcaataat attcctatag aacacatgaa attatcaagt
    18601 gaaattatag aatacgcaca aataaagtta aacaagaagc taaatgaaag tatttacata
    18661 tccttaagta gaccatacat attctgcaat tcaaaggatg aaagaaggaa taaatgtaaa
    18721 aaatgcaatc ctttgggaaa ttaaaagatt ttataaagaa gagtttgaaa ttggaatgaa
    18781 agcactagat attattgaaa acaaaacagg tataaaatta ccagaagatg aggctggatt
    18841 tatagccttt catatagtga atgctcagtt aagtgaaggt catactttgg catctgatat
    18901 tacgaagtta atacaagaag tacttagcat agttaggtat cattttagga tagaatttca
    18961 tgaagaatca gtgttttatt atagatttat aatgcattta aagttttttg cgcaaagatt
    19021 gcttttagat agtgcacatg aaggtgagac agacaaagaa ttactaagca ttattaaatc
    19081 taaatataat aaagagtttg aatgtgtagt taaaattaaa aatttcatta aaaaacaata
    19141 taactacata ttaacagatg atgagcttat atatttaaca atacatttag ctaaagtagt
    19201 taaagactca aatatgtaag tgaaataatt tttcagtagt atattaagaa tgattgaaag
    19261 gagtgatact aacaaaatta tacttatggt tggatggtaa cattgagttg gccaaacctt
    19321 caaatttcaa aaaattaatt taaacgaaat atggaggaga aaaatgggaa aatacaaaca
    19381 attagcacaa gagatagtaa acaatgttgg tggaaaagaa aatattagtg ggttagtaca
    19441 ctgtattaca agattacgtt ttactttaaa agatgaaagt atagcaaatg acaatgtatt
    19501 aaaaaacatg gaaggtattg ttactgtaat gaaaagtgga ggacaatatc aagtagttat
    19561 aggaaatcat gttgaagctg tctataaaga tgtagttgaa ataattggtt tagatgattc
    19621 aagtacaagt tcagaaacta aaaaatcagg taatatacta gataaaggta tagatataat
    19681 atcaggaatt tttcagccag tgttaggaat aatggcagct tgtggtatgt tgaaaggatt
    19741 taatgcatta tttgtagcaa tgggattata tagtgatgta tctggtggtt atatggtaat
    19801 aaatgcagca ggagatgcat tatttacatt ccttcctttg ttcttaggat atacttcagc
    19861 taagaagttt ggattaaaac caatgttggg attagcacta ggagctgcag tatgttatcc
    19921 tgctatacaa ggtagcagta tatcagcagg agcagatgtg atgtacactt tatttaaagg
    19981 aacaatgttt gcatcaccag tatatattga tttctttgga ctaccaattg tatcaattga
    20041 ttatactgga acagtcgtac caattatatt tatagtgtat tttgcttcta ggtgtgaaaa
    20101 attatttaat aagtgtgttc caaatttagt taagtttttc tttgtgccaa tgcttacatt
    20161 attaataaca gtgcctgttg cacttatagt tataggccct atagcaacat ttggttctac
    20221 acttatttca caaattgtaa tttcaataag agattttagt ccactattgg caggtgctat
    20281 agtaggattt acttggcaaa ttcttgttat ttttggtatg cactggggat ttattcctgt
    20341 atatataaac aatgttatga ctttaggata tgataatgtt atgatgccat tctttgcctg
    20401 tacatttgca agttcggcag ttgtgttggc aatatttttc aagactaaag ataaaaaact
    20461 aaaagagatg gcaataccaa actttatatc tggtatattt ggagttacag agccagcaat
    20521 ttatggaata ttattgcctt taaagaaacc atttataata agttgtattg ctagtggtat
    20581 tggaggcgct ttctatggat tctttaatct tagaaaattt ataactggag gaatgggaat
    20641 atttgaactt ccagcaatga ttgaaccaga tgggggaatg ggtaacttaa tagtagctct
    20701 ttctggtata gttatatcaa tggtcgtagc ttttgtatta actatgattt tatataaaga
    20761 tgagaaagta gaagagtcta aaaaagtaag aaataagggt aaagaagaaa taaaagaagt
    20821 caagtctact aaattagaaa gagaaatagt aacaagtcct ataaaaggtg aagtattaaa
    20881 attaagtgat atagaggatg cagcatttgc atctggtgta ctaggtcaag gggtggcaat
    20941 aatacctagt gatggaaaag tagttgctcc agtagatggg gtagttacaa ccttatttcc
    21001 tagtctacat gctataggga ttctttctga tacaggtgtt gaagtgctta ttcatattgg
    21061 attaaatact attcaattag agggaagagg ttttgaggca tgtataaaac aaggtgatag
    21121 aattacaaaa ggtcaaacta tattaaactt tgatgttgat gcaataaaag agttggggta
    21181 tagtactgta actccaattg ttattacaaa ttcatcacaa tttttagatg tagtagaaac
    21241 agaaagtaaa aatattgaat tagaagacaa tctaattaca gtcttatttt agtaaagatt
    21301 gaggtattag cataatctag tgctttggtt ataattttag gaggctttga ctatgagttt
    21361 taagaatgat tttttatggg gaggagctac tgcggccaat caatgtgaag gtggatacaa
    21421 tgaagacgaa agaggattgg caaatgtaga tgtatgtcca acaggaaaag atagaacagc
    21481 agttattaca gggaaactaa aaatgtttga ttttgatgat gagcattatt atccagctaa
    21541 aactggtatt gatatgtatc ataattataa agaagatata aagttatttg ctgaaatggg
    21601 atttaaagta tatagaatga gcattgcatg gagtcgaata tttccaaaag gtgatgaaga
    21661 aacaccaaat gaaaagggac ttcaatttta tgaaaatata tttaaggagt gtaaaaaata
    21721 taaaatagag cctttagtca ctattacaca ctttgattgt ccaatgcatt tagttaaaaa
    21781 gtatggtgca tggcgtaatc gaaaaatgat aggtttctat gagaaattat gcaatgttat
    21841 ctttagaaga tataaaggat tagtgaagta ctggctaact ttcaatgaaa taaatatgat
    21901 tttacatgct ccatttatgg gtgctggtat ttgctttgaa gaaggtgaag atgttgaaaa
    21961 gataaaatat caagctgccc atcatgagct tgtagctagt gcaattgcaa ctaaaatagc
    22021 acatgaagtt gattcaaata acttaattgg atgtatgttt gcagctggaa gtgtatatcc
    22081 ttatagttgt aatcctaatg atgtatggga agctacaaag ttagatagag aaaattactt
    22141 ctttgtggat gtacaatcta aaggtaaata tcctaactat gcgttaaagt atatggagca
    22201 aaaaggtatt acaccagaaa tggaacctgg tgatattgaa ttattgaata aatatacagt
    22261 agattttatt tcattttcat attacaatag ccgttgtgtt agaaccgatg aaaatgcaga
    22321 tgatatggct gaaggaaaca tatttacatc tgctaaaaat ccatatcttt catacagtca
    22381 atggggatgg ccaattgacc cattaggttt gagaattaca ctaaatcatg tatatgatag
    22441 atatgaaaag ccactattta ttgtagaaaa tggattaggt gctaaagata tagctgatga
    22501 aaatggttat gtagaagatg attacagaat tgattatata agagaacata taaaagctat
    22561 gcatgatgca gtttctatag atggggtcga tttattaggt tatactacat gggcacctat
    22621 tgatttagta agtgctggta caggtgaaat ggaaaagcgt tatggattta tatatgtaga
    22681 tagagacaat agtggtaatg gaagtctaag aagaatgaaa aagaaaagtt ttgaatggta
    22741 taagaaagta attgcttcaa atggtgaaga tttatattag acaagaaatc tatgttaaat
    22801 aagaaatttg tattaaataa gaagagtata aaaattaata ataagtataa tttttatctt
    22861 ttttaactat agaaaatcaa tacagaatta ttttacattg tatttatgca atagtatgat
    22921 tcacaaataa aattttagaa aataaaattg ttaagaagta tgggaagtaa gtgcaattta
    22981 ctttgtaata aaagatgaaa aaggcaagct taggttttaa gcttgtcttt tttattatgt
    23041 ataaactatg atactttaaa attctatgaa aaatttattt aatagagaaa ttaaaaattt
    23101 aatggtaatc aaattcttta ccaatgaata taaaaaatgt ctacgtgtta taatagttta
    23161 aactaataaa cgaaaggtag atgaatgaat atgaaatacc aacatataat agaagttgac
    23221 aaagaacttt gcattggatg tgggctttgt aaaaatgatt gtccagtaaa caatataatt
    23281 atagaaaata aaaagtcagt aattaaaaaa caagattgtc taatgtgtgg acattgtgca
    23341 gcaatatgtc ccacaaaagc tattaccttg acaggatttg atgaacctcc tatagaactt
    23401 acaaataagc caaagctaga ttcagatgaa ttactaatgg caattaaatc tagaagaagt
    23461 atacgtaaat ttaaagacaa agaagtttct tcagaaatta taaagcaaat tattgaggca
    23521 ggtaggtata cccctagtgc taaaaattca caagatgtat catatattgt tttagacaat
    23581 aaaaagtcaa tttatgaaaa cgaagcagtg aaatttttta gaaaaataaa acctattgca
    23641 aatatagcta ttaaatattc aaaagaagtt gagattgacg ataacttttt ctttaagcat
    23701 gctcctattg caataatgat aattacaaaa gataagatta gtggttcact tgcagcatca
    23761 aatatggcat taatggcaga gtcttatggc ctaggtgttt tatttagtgg atatttttca
    23821 gatgtagcaa ataattcgcc aaaattaaaa aagcttttag gtctaaaacg cagtaatcat
    23881 gttcttacaa cattagtaat tggataccca gatgttaaat atagacgtac tgcacagaaa
    23941 gaagtagcaa ctgttagata cttataagag agggtggatt ttatggaaaa agaatgtcaa
    24001 caaagaatgc aagaaataac gtctaaattt aaagaatgtc gcaaagctat ctctgctatg
    24061 ggtgatgaga ctagacaatt gattttaatt gctctcttag agagtgattt taatggtata
    24121 cgagtgggag aaatcacaaa aaaaacacat ctttctcgtc ctgctgtatc tcatcatctt
    24181 aagatattaa aagaagcaga gattgttaat ttacgtagag aaggaacgaa aaattattat
    24241 tatcttgatt caaaaaaatc acaatggaag tcactaacag agctaataaa tcttatattt
    24301 gttggaatac aacatattag tgaaaatgat ataagaaaag gtgattaaat tatacttgat
    24361 ttaatacaaa aagattgatt taggaactaa aaaattcact ttgagacact atcttttgaa
    24421 ctatacccat tttaggagac ggttaaaata tgtaaaactg tataaaacaa tgggtatagt
    24481 tcattttata atggtttaaa tattaagaat atattattct aaatagtatc tttctctgtt
    24541 ttgagtttta tttttatact caatagcttc agtggggcaa ccacaaatac atgccataca
    24601 atgagtacag ttatttttcc aaactggttt tttgtttttt aaattaatat tatttaaagg
    24661 acatagctct acacattttc cacagccaat gcatttatca gtataatgaa atcctttggc
    24721 actaataatt gtcttataaa aaactgtatt tattatacca cttttaattg tacctcctaa
    24781 gccactagga gtaatagttt caaattcatt ttcattgcgt atatcagaaa taatcctata
    24841 tatttgttta tctgcttctt taatcatttg ttttgatgtt tctttgtctg ttgtagaaaa
    24901 taaagctata taattctctg gcattttaat ttctgccata ccttttaatt gccattcctt
    24961 gtatttacat agtttttgaa tatagtttat ggattttgct gtatctccac cacaagtcat
    25021 tacaaagtaa actctgttgc ttcctagaaa ttttgtttct tttataaaat ccgtaaccac
    25081 tattggcaat ctccatgcat aagtaggaca tacaaatatg aatggtttat ctgttgatat
    25141 taattcatct gttttttcct cttttataag ttgatttagt gatatcagtt catcatttaa
    25201 ttcttgtgca attttacgag ccacatatcg gctattacca gttccgctaa aatataaaat
    25261 cataataata tctccctaac tattttttat tttaactctt aattttttat ttattatagt
    25321 aatttattga tatattctat tcaaatgtat gtaaaattta gtagttaaga aaattataac
    25381 acattagtaa tttttatact tattggtaaa gaatttgatt accattaaat ttagcaacaa
    25441 gacatcaact ggacttgtat atgagaaggg ataataattt gcttatgaaa gattataaat
    25501 ataaaggtta ttttgaaggt gtagaaatat ttgataatgt aaatgacctt aacttaaata
    25561 ctttaacaac agttagtaga tttgtgtttg tttttttgtt tacgataact taaggaaata
    25621 tgttacctga acaataaatt tatataataa aagtatataa atatatatat gaatgttaag
    25681 attagttgat agaatttaaa taataaatcc aatattataa acataggagg tatgaaaaat
    25741 atgattaatg agaatttaaa aagtctaaga aaaatcaatc agtatacaca agaagaatta
    25801 gctgaaaaac ttaatgtttc tagacaatca atagcaaaat gggaaagtgg agtttcccaa
    25861 acaaaaggat attaaggaat aacctgtgtt tacctgcttg caaaaatact gaatataaga
    25921 gaacagtact tgaaatatag tgtttttcct atgtgttata aattttactt gatagtctaa
    25981 aagtaatgac aggtcgtttt ttatttagaa aaaagattaa aaatctgatt atctgcataa
    26041 acacaagtac tttaagtaat aggaatgaaa atacaaaaga aatttaccac gattgaaaga
    26101 accttgatac gataaagaaa aatcgcataa aaaagaaaaa aggtatttca tacaagatat
    26161 gagatgtctt tttgttgctg taacacagca taatttgttc ttttttctgc ttgattatct
    26221 atcatttttt agagcatgaa ttgtcaagag cttgttgcgt aagcaatgct tgcactcttg
    26281 ataaggcatg ggataaaaaa tattctgaca gatagcagaa aagtaatgaa ctgaaatgag
    26341 gtaagtgcta taatatattt atcaattttg gaaaacgtgg agagatatat ggaaaagata
    26401 ttagaaaaca cattattact catgatactg ccatggatat ttgtctgggg aatactatgt
    26461 atcttagaaa agagaaaacg gaaaaatgaa gacggaaata aagatgaaaa aagctatgtt
    26521 atacaaaatg ataatatatt ctttcccttt ttttcgttag gtatgttttt attttcattt
    26581 tgcctttttt atacggtgtt ttttactgaa tttgatatat cggaagtaat atcagtttct
    26641 gtctttgtca tattttttgc tattttaaca ttagatagtg caatctggag agtaattgta
    26701 gatggtgata taatcaaata ccgttcattt tatgggatta ctaggacata taattttaat
    26761 gatattacca aaggagttta tactaagaaa ggttctttaa aaatatatat tgaggggaaa
    26821 agaattttta cattttatga taatttagaa ttttctttat ttgaaaatca aatgagaaaa
    26881 agaaaaattc ctttagaata taaacgcaaa gaaaaataat aaggaaattg ttcagaatta
    26941 gtttaataat attaccactt taaagacgga gtgatccaga cacggtggca gggaaatctt
    27001 tcagattttt ctgccatgtc ttgcaagtgg ctggttcact tcgtctgtga gcgagcctgt
    27061 ggcgagcgaa gattaagccc tcgttatcta acaagggggg cacaaaaagc aagagacaag
    27121 agacatttac aagcgaaaaa cgctatatat acaaggaaaa gtcgctttta atatgatgtg
    27181 atataaatta tgccattatc actatgaaaa aattagaatt catattttat ggaggtgaaa
    27241 gtttgataat aaaatttttg gaaaagaaac tcacaatatc agggaaatta gcaagagttt
    27301 taggaactat cctgatggtg ttaattggta cttttttact atattgtgca ataaatccgc
    27361 caacatattt tcactttctc aaagagaata taaatcgaat tatatacgga gtaatcggag
    27421 ttcttttaat ccttaaggca ctaagaatgc tttttacgaa taaattaaaa aataaatcat
    27481 aaaatttcag ctttaaaatt tcatattcat acacacaagc agttagtctt aggattgact
    27541 gctttttcta tacagatttt tattgacagg caattatcaa gatacggtag ttgccttttt
    27601 ttaattggag gaattgaaga atgaatgata aaaactttat tgaagaatta agacaaaaac
    27661 gtgaagatta cggagtaaca caaacaaggc ttgccattgc ctgtggtatc agccgtgagt
    27721 attacaaccg cattgaaaaa ggaaaacaat cattgaatga cgagttaaaa gaaataatag
    27781 aaaaacagat tgagcgtttc aatccacaag aaacattatt cttattgatt gattactttc
    27841 gtgtccgctt tcctactacg gacgcattaa agattattcg tgatgtatta caactgaaag
    27901 ctgattatat gctttatgaa gattttggaa agtacggata tgaaagcaaa tatgttttag
    27961 gagacatcaa tatcatgtgt tctatgcaag agcatttagg cgttttgtta gaactgaaag
    28021 gcagagggtg tcggcaaatg gaaagttatc tattggcaca ggaacgcaca tggtatgact
    28081 ttatgcttga ttgtatgacg gcaggcggtg tgatgaaacg gcttgacttg gcaatcaatg
    28141 ataaagcagg aatattggat attccaaaat taaaggcaaa atataaagca ggagaatgta
    28201 tttcctactt tcgtatgcag aaagattaca gtggtacaga gaaatgtggg agtgatacac
    28261 caaagaacac aggagaaacc ttatatcttg gttcaacaag tagcgaatta tatatgtgtg
    28321 cttatcagaa gaattatgag cagtatgtca agaatggcac agaagttgaa gatacagaaa
    28381 tcaagaaccg ttttgaaata cgcatgaaga atgaacgagc ctattatgca gttgtagatt
    28441 tactgaccta tcgggacgct gaacgcaccg ctttttctat catcaatcat tatgttcgct
    28501 ttgtagatag agaggacgat aaacctaaaa gtcaatggaa aatgaatgat gattgggcat
    28561 ggtttgtagg ggataacaga gaacctatac gcttaaccac aaagccagag ccttatacct
    28621 tacaaaaggc attgcattgg ttacaaagac aagttgcacc aacgataaaa atggtacagg
    28681 cattggataa agaaaatcat acaacgatac tgaaagatat gattgagcag gcagagctca
    28741 aagacaagca taaacattta ttacagttag aaaaatcaac catagaagaa cgcatagata
    28801 ccgttgttcc acaagagaat gacggtattt tttaatttct ttgaaaaatc tgcaacaaag
    28861 cattaactag cgtacagata tgctgcgaga aaaggaaatc taactttttt caaaaccact
    28921 cattaaattg aggtttccat tccctatatg gagtgaaaga agaaaatatt ttcattctca
    28981 acttaacatt tggcagttgc cgttccctat atggagcaaa gaaaatcttt cattccatag
    29041 ttggcaataa cggtgttgca tgggtagtta gggtgagaaa agaaattttt tcatcagtaa
    29101 cttagcagaa tgacactttc tttccctata tgatacagga aaagaaaaaa gttttgaata
    29161 ttggttacgc ttttgctctt ttccaacgcg atatatagga aagacgattt tcttctttcg
    29221 tgttctttga caactgaata aagcaattca ataccttaga cagacagcga gccgttgaaa
    29281 cagatacgcc atgacctttc ccctgcaaaa gcgagcgaaa atctatttgt gttcttgtaa
    29341 aagacttgtc ctcttttatc gctatgacct gttctgtctg ataatgatac tcccgtacag
    29401 ccacagcttg agcgttaaga gcgtcgcacg caatgggtac ggctacattg gataatgcag
    29461 aggtggaagt cctgtgggct atgacaaaag ccgttgaatt gcttagaaag attttacaga
    29521 aagggggtat gtgttattga taatactgta aaaacaattc aaaagaaaaa taacaagtgt
    29581 ggcattggaa acagaggaaa gacaaagatt gttgtaaaag agcatttttc cgaaaaagga
    29641 aagacaatgg aagaacttct gactgatgtt atgctggaaa aagcaaagca gacaatcgca
    29701 taaattcggt gcagttgtaa gaaatagatt gaatggcaaa aagtacccgt gttatacttt
    29761 acttgcaagc aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa
    29821 caacagattt acaatactgc actttatctt aggttaagcc gagatgatga attacaaggc
    29881 gaaagttcta gcattaccac acaaagaagt atgttgcgtc tatatgcaaa agaacatcat
    29941 ttgaatgtga ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgacagaccg
    30001 agttttcaga gaatgattga ggatatagag gcaggaaaaa tcaactgtgt tgtaacgaaa
    30061 gacctttccc gacttggcag gaactatatt atgacaggac aatatacaga attgtatttt
    30121 cccagccata atgtccgtta catagcgatt gacgacggtg tagacagcga aaaaggcgaa
    30181 agtgagattg caccatttaa gaacatcatc aatgaatggg tagcaagaga tacaagccgt
    30241 aaagtgaaat cagcgtttaa gacaaaattt gcagagggtg ctcattatgg ggcttatgct
    30301 ccgttaggat ataagaaaca ccccgacatc aaagggaaac tgctggttga tgaggaaaca
    30361 aaatggattg ttgaaaaaat cttctcccta gcctatcaag gttacggtag tgcaaaaatc
    30421 acaaagatac tgcgagaaga aaaagtcccg acatcgtctt ggctgaattt tacaaggtat
    30481 ggcacttttg cacatatttt tgagggcaaa cctgaaagca agcgttatga gtggacgatt
    30541 gctcatgtca aggcaatctt aaaaagtgaa gtctatatcg ggaacagcgt tcacaatatg
    30601 cagtctacgg tatcgttcaa gagcaagaag aaagttcgta agcccgaaag tgaatggttt
    30661 cgagtagaaa acacgcacga accgattatt gacaaggaag tgttctatcg tgtgcaggaa
    30721 cagataaaat caaggcgtag acagacaaag gaaaaggcaa cgccgatatt tgcagggctt
    30781 gtcaaatgtg cggattgtgg ctggtctatg agatttgcga caaataaagc aaataagaca
    30841 ccatacagtt attattcttg cagtttctac ggacagtttg gtaaaggtta ttgttctatg
    30901 cactatatcc gctatgatgt gctgtatcaa gctgtattgg agcgattgca gtattgggct
    30961 aaggcagtac agcaggacga agaaaaggta ttgcataaga tacagaaagt cggcaatgca
    31021 gagcgaatac gggaaaagaa gaaaaaggca aatgccttga agaaagcgga gaaccgacaa
    31081 aatgagattg accgtttgtt tgcgaaaatg tatgaagata gagcctgtga gaagataaca
    31141 gaacgaaact tcattatgct gtcggggaaa tatcaaaagg agcagataga actggaacag
    31201 cagataacca acctaagaga agaattaagt aaaatggaac aggatatgat aggtgctgaa
    31261 aagtggattg agttaatcaa ggagtattcc gtaccaaagg aactgacagc accgttatta
    31321 aatgcaatga tagaaaaaat actcattcac gaagcaacaa cgaatgagga gaacgaaaga
    31381 atacaggaaa tagagatata ctaccgattt attggaaaag tagactgata aataggggtc
    31441 atatctttaa ctaagggaaa gcggagaatc tattcctgat ataggtagtt gcattaaact
    31501 tgcaaaattg tacaatgtta aactagatga cttagttaat cattcggaag aaaaaacagg
    31561 tattatagta cctcctaaag gaaagttttt ctttggagca gttgtagttg gtgaacgtgg
    31621 tcagattgtc attccaaaag aagcaagaga agtatttaat ataaatgcag gagataaatt
    31681 attggtactt ggagatgagg aaagaggatt aggaattgtt catcaacgtg atttgataaa
    31741 ttttataggt catgtgggag tagtacataa agaagaatga tttaaaaaaa ttataaaagg
    31801 aggtatgaaa tatgagtaaa atagtatttt ttagcattcc tgcatatggt catactaatc
    31861 ctactattga agtagttcgt gaattagtgg atagaggaaa tgaggtattg tattattctt
    31921 ttaatgaatt taaagataaa attgaaggtg ctggagcaaa atttatttgt tgtgataaat
    31981 accttccaga attactccct ggagatgaga aaaaaattgg taaggatttt cctggattaa
    32041 ttgagatgat agtagatact actatttctt tagatgaaaa agtttgtaga gaacttaaag
    32101 attttaatcc agattgcatt gtatcagatt cattaagttt ttggggaaag ttatttgcac
    32161 aaaaacttaa tattccatat gtatgctcta caacaacttt tgcatttaat aaacatacag
    32221 caaagttgat gaaacaaaac tttatagaaa ttatacgtat gatttttggt gtaagacgca
    32281 ttaataaaaa aataaagctt ttacaaagaa aagggtatga agtaaaaaat tttatttcta
    32341 tagtttctaa tgataatgat acggatacca ttgtatatac atcaaaagaa tttcaaccaa
    32401 tggtagaaac cttctctagt aagtattcat ttgtaggtcc atcagtatca aagttgatta
    32461 ttgaacctaa agagagaaaa agaaagctaa tatatatttc gcttggtaca attaataata
    32521 aaaatgttag cttttataaa aactgtataa gtgcatttaa agattctgat gttgatgtga
    32581 ttatgtctgt tggtagaagt actgatatta aaagtttagg aaatatacca aataattttg
    32641 aggtcaaaaa tagtgttgaa cagattgcta tacttcaaaa aactgatgtg tttgttactc
    32701 attctgggat gaatagtgtt aatgaaagtt tatattatgg aatacctatg gtattatttc
    32761 ctcaacattc ggagcagaga atggtagcta agagagttgt tcatttggga gcaggaatta
    32821 tgttaaaaga ggataagtca gaaagtatta aaaaagcagt atttcaagtt ataaatgaca
    32881 atgaatataa agaaaatgca actaaactat caaaaagctt ttataatgca ggaggctcaa
    32941 aaaaagcagc agatgtaata ttacagatta tatgtaatag tagacaagtt gacatataat
    33001 agatttgtta aaaatatata tacatacatg taatttaatc aaagtactga cagtggggtc
    33061 ttttaaccta ccagttgggg catatatatt gatgctaaat atgagggatg ttatatttta
    33121 ataaaaaaga taacatcctt tttttagtgc attatatcga gttttaaata ataaatgaag
    33181 gaggattaat atgaaaaaat ataaagttat ttttatagta ttaattatag gtattataag
    33241 tgtaacaggg tttgttgtat ataaaaactg cattaaacca tggaaagatg ctgaaacata
    33301 tattgataag tatatggtaa aacaagatat ttcaaaagat aatataaaaa gtattacaaa
    33361 agaaaaagcc aagaaggcaa gttacgaagg aatactatac aaagtgtatt acaaagatga
    33421 ccctcgatat gagtatcaat atttttatag tgataattat tatgctctat atgtaaataa
    33481 agtaatgtta caaatttatg atatggaaaa taataataaa cttttaagaa gtaatgagga
    33541 attaaaaagt gtaaagtatc caccaatata tcttaaatat aagccagata aatgatttat
    33601 gacaaaatag aatgattttt attaaaatt
//
LOCUS       DAEMIK010000027        33487 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEMIK010000027 DAEMIK010000000
VERSION     DAEMIK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.027"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            338..1129
                     /locus_tag="KPK87_001098"
     CDS             338..1129
                     /locus_tag="KPK87_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG2537267.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            1158..2378
                     /locus_tag="KPK87_001099"
     CDS             1158..2378
                     /locus_tag="KPK87_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG2537268.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2398..3093
                     /gene="pxpB"
                     /locus_tag="KPK87_001100"
     CDS             2398..3093
                     /gene="pxpB"
                     /locus_tag="KPK87_001100"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG2537269.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEVEKNNLSI"
     gene            3123..4109
                     /locus_tag="KPK87_001101"
     CDS             3123..4109
                     /locus_tag="KPK87_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG2537270.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4328..4894)
                     /locus_tag="KPK87_001102"
     CDS             complement(4328..4894)
                     /locus_tag="KPK87_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537271.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5246..6313
                     /locus_tag="KPK87_001103"
     CDS             5246..6313
                     /locus_tag="KPK87_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG2537272.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIRKAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6332..7492
                     /locus_tag="KPK87_001104"
     CDS             6332..7492
                     /locus_tag="KPK87_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG2537273.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7505..9601
                     /locus_tag="KPK87_001105"
     CDS             7505..9601
                     /locus_tag="KPK87_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG2537274.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPFEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9760..10104)
                     /locus_tag="KPK87_001106"
     CDS             complement(9760..10104)
                     /locus_tag="KPK87_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2537275.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPSRACVEARLAREH
                     LLVEMSVVAATK"
     gene            10396..11286
                     /locus_tag="KPK87_001107"
     CDS             10396..11286
                     /locus_tag="KPK87_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2537276.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11504..12790
                     /locus_tag="KPK87_001108"
     CDS             11504..12790
                     /locus_tag="KPK87_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2537277.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12879..13322)
                     /locus_tag="KPK87_001109"
     CDS             complement(12879..13322)
                     /locus_tag="KPK87_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG2537278.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13346..14140)
                     /locus_tag="KPK87_001110"
     CDS             complement(13346..14140)
                     /locus_tag="KPK87_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG2537279.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14706..15182)
                     /locus_tag="KPK87_001111"
     CDS             complement(14706..15182)
                     /locus_tag="KPK87_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2537280.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15283..16452)
                     /locus_tag="KPK87_001112"
     CDS             complement(15283..16452)
                     /locus_tag="KPK87_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2537281.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVILHVKNVLSF"
     gene            16615..17478
                     /locus_tag="KPK87_001113"
     CDS             16615..17478
                     /locus_tag="KPK87_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG2537282.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17549..18142
                     /locus_tag="KPK87_001114"
     CDS             17549..18142
                     /locus_tag="KPK87_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG2537283.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18392..19027)
                     /locus_tag="KPK87_001115"
     CDS             complement(18392..19027)
                     /locus_tag="KPK87_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2537284.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNKVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(19107..19838)
                     /locus_tag="KPK87_001116"
     CDS             complement(19107..19838)
                     /locus_tag="KPK87_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG2537285.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMNIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLTNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            20169..22358
                     /locus_tag="KPK87_001117"
     CDS             20169..22358
                     /locus_tag="KPK87_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG2537286.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            22769..24697
                     /locus_tag="KPK87_001118"
     CDS             22769..24697
                     /locus_tag="KPK87_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG2537287.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            24684..25031
                     /locus_tag="KPK87_001119"
     CDS             24684..25031
                     /locus_tag="KPK87_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG2537288.1"
                     /translation="MSKSNDVLDIVFKITVGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(25273..27192)
                     /locus_tag="KPK87_001120"
     CDS             complement(25273..27192)
                     /locus_tag="KPK87_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2537289.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(27218..28024)
                     /locus_tag="KPK87_001121"
     CDS             complement(27218..28024)
                     /locus_tag="KPK87_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2537290.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            complement(28083..29531)
                     /gene="ddlR"
                     /locus_tag="KPK87_001122"
     CDS             complement(28083..29531)
                     /gene="ddlR"
                     /locus_tag="KPK87_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG2537291.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(29555..30454)
                     /locus_tag="KPK87_001123"
     CDS             complement(29555..30454)
                     /locus_tag="KPK87_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG2537292.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            30712..31968
                     /locus_tag="KPK87_001124"
     CDS             30712..31968
                     /locus_tag="KPK87_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG2537293.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            32026..32805
                     /locus_tag="KPK87_001125"
     CDS             32026..32805
                     /locus_tag="KPK87_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2537294.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(32909..33487)
                     /locus_tag="KPK87_001126"
     CDS             complement(32909..33487)
                     /locus_tag="KPK87_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG2537295.1"
                     /translation="MNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYKLK
                     KHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSERW
                     EFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMNDL
                     KFSAYTCNSVINYILDSILSNTIKSYHIANCG"
BASE COUNT        12379 a         4065 c         5408 g        11635 t
ORIGIN      
        1 aagaaaaatg attctgtctt gcatataaag tgattaaaaa tgaaaaatac tttaaaaatg
       61 aaggttaaag cccttatttt aagtgaaaat aattaattat taactaaaaa atggtaaaaa
      121 gattttttga aaaagaaata caaatgaatg ttcaattaaa ataattgcaa aaatattcct
      181 taaatggtaa aattatatta attaaatgca atagaatagt tgcaaaagta aaattgaata
      241 caagtgataa aatatttcat gcaatatata aaataaacaa aatttaaaaa ttaaaactac
      301 ttattctatc aaaatgttag caaggagggg tcataaattg tataaagtag atttaaatag
      361 tgatttagga gaaagttttg gaacgtataa gattggttta gatgaagaag ttttaaaata
      421 tatttcatca gctaatatag cttgtgggtt tcatgcaggt gaccctagtc atatggaaaa
      481 aacagtacag ctagctaaaa aaaatggagt gaaaataggc gcacatccag gtttcctaga
      541 tttaatagga tttggaagac gtgagatgaa aataacaaag caggaagcta aagattatac
      601 aaaatatcaa ttgggtgctt taatggcatt tgcaagttca aatggatgta atatacaaca
      661 tgtaaaacca catggagcat tatacaatat ggcagctaaa gataaagagt tagctatggg
      721 gatttgtgaa gctatatatg aagtcgataa agatattata ctgttggggc tatataatag
      781 tgaaatgata aattctgcta aagaaattgg acttaggttt gcaaatgaag tatttgcaga
      841 tagagcttat gataataatg gttttttggt tccaagaaat gtagaaggag cagttataca
      901 tgatacaaaa catgcaatag atagggttgt taggatggta aaagaaggaa ctgtagaaac
      961 tttaacaggt gaagttatac atatcaaagc agattctata tgtgtacatg gggacaatcc
     1021 aaaagcaata gagtttgtca aagaaataag aaagcgtttt gaattagaaa gtatagaagt
     1081 ttgtccatta gaaaacatag aagtttgttc gtcagagaac atagtataag actgataaca
     1141 tgagggggag ataaaatatg agtacaaaaa atattacaga taaaactaaa aataaaaaag
     1201 atgtcggagc acttataggg gctgcattta taatggcaac atcagcaatt ggaccagggt
     1261 ttttaactca gactgcacaa tttacccaag attttggacc gaatttttca tttgtaattt
     1321 taattacaac aatccttttt ataggggctc aagtaaatgt atggagagta attggtgtat
     1381 caggacttag aggtcaggat atagcaaata aaataatacc gggattaggt tatttagtag
     1441 cttttttagt agctcttgga ggattagctt ttaatatagg aaatgttggt ggagctgcac
     1501 ttggtatgaa tgtaatgttt aatatgaata tgacattagg aactgtatta agtggattaa
     1561 tagccatatt tgtatttatg tcaaaaaatt caaattctct agtcgataag ataactaaat
     1621 ttttagcatt aggaatgata attatagttg gatatgtagc aatatcaaat catcctcctg
     1681 ttggagaggc agtatcaaga atggtaaaac cagaaaatcc aaaaggactg atattcccaa
     1741 taataactct acttggagga agtgttggag gttatataac ttttgctgga ggacatagat
     1801 taatagacgg tggaataaca ggagaagaaa atataaaaga aataactaaa tcttctcttt
     1861 tgggtatatt agttgccaca atgatgagag ttcttttatt tttagcaata ctggcagtgg
     1921 tttcaaaagg tcttcaactt gacccagaaa atccagctgc ttcagctttt aaatttagtg
     1981 caggagcaat aggatataaa ttctttggac ttgtattatg gtctgctgca attacatcag
     2041 ttataggagc tgcttataca tcagtttctt tcttaaagac actaaatcct tttatagata
     2101 aatatgaaaa atattttata atagcattta tagcaatatc aacacttata atggcattta
     2161 taggaaaacc agctacttta ttaattttag caggagcttt aaatggattg atacttccaa
     2221 tcacattagg aattatgctt atagcatcta aaagaaaaga tattgttgga gattacaagc
     2281 atcctacatg gcttttaata tttggattaa tagtagtttt aatatctgca tatacaggaa
     2341 taacttctct tagcagccta ggtgctttat ttgcttaatt tagaagaggt gacatttatg
     2401 gaaactagat acttgatatc aggggataag gcagtggttg ctgaatttgg aaatgagata
     2461 tcagaagata taaacaaaaa agtaatttca tttatgagag caatagaaat atcgaatctt
     2521 aaaggtattg taacagaaat ggtaccaaca tataggtcac ttatgataag ttataatcca
     2581 ttagaaatag attttgatag tttgattgag aatttaaaaa aaatagaaga taatttggaa
     2641 agtatagtat taccaaaacc taaaatacat gagattccag tatgttatga tgaggttttt
     2701 ggtatagata ttaaaaacgt tgcaagctac aataatctca ctgtagatga agttataaaa
     2761 atacacacaa gtagagagta cttgatatat atgctagggt ttactcctgg atttccatat
     2821 ctaggtggta tggatgaaag aatagctaca cctagattag aagtgccaag gactaaaatt
     2881 tatggtggga gtgttggaat agcaggttcc cagactggtg tatatcctat tgatagtcct
     2941 ggtggatggc aaataattgg taggacacct ttaaaattat atgatgaaaa tagagaagaa
     3001 cagattttgc ttagagctgg agactttata aaatttgttc caataacatt agatgaattt
     3061 atagaagtcg aaaaaaataa cttaagtatt taaatatcgt tatagtacag ggagtggtat
     3121 atatgggatt caaagttgaa ttaggaggtt tccaaacatt aatacaagac agaggtagag
     3181 ttggctatgg tcaatatggt gtatctggat gtggagcaat ggatgagtat gcacatagag
     3241 taggaaatat attagtagga aattctgaag atgaggcttc tttagaagtt ttaatgttag
     3301 gtcctacaat tacctttgat gaatatacgc aaatagcagt aactggggga gatttagggg
     3361 ctaaaataaa tggaaaagaa attcaaaact ggaggtcata tcaaataaat ccaggtgatg
     3421 tattatcatt tagaggtgtg aaatcaggtg ctagagccta tgtgtcaata gctggtggaa
     3481 tagatgttcc acttgcaatg ggaagtaagt caacttatac aagagctaaa attggtggat
     3541 ttgaaggtag agcacttaaa aaaggagatt atataaatac ttttattcag gaaaaagatt
     3601 ttacaataaa taaaaaatta agttcaaaat atattccaac ttatagttca gaaatcgtac
     3661 taagaattgt aaaaggtcct caatttgatg ctttttctaa cggtgaagta gagaaatttt
     3721 tatcaaataa atacaaagtt acgaatgaaa tagatagaat gggttgtaga ctagatggag
     3781 agagtataaa gcaccttaat ggagcagata ttatatctga tggtatttct tatggagcta
     3841 tacaagtacc tggtcatgga aaaccgatta taatgttatc agatagacag actagtggtg
     3901 gctatactaa gatagggaac gttataagtg tagatttgta taagttagca caagcaaagc
     3961 caaatgatgt agtaaaattt gaacttgtag atatatatga agcacataga ctattgagag
     4021 agcaagaaga taagattcaa gatatctaca agagtatgaa aaatataaga gttgtaaagg
     4081 caaaagtatt aaatgatata gcagtttaat ttaattttaa caaatacaat ttttattata
     4141 atttataata taacttacaa atagctattt actatttacc ataattaaga tggtttagat
     4201 tagtcaaata gctatttttt tagtttgagt aaatatttta aatttaaata aatacttcaa
     4261 ttttaaaata aaaccgatat ttaaatttat tgtattatct acaaaaagta ggtgtgttat
     4321 attgagttta tttagatttt attggccaaa ttggaggtga tataactcca tatccacgag
     4381 ctattgtatc tttacttgga ttaatgattt tatgtggtac attagctttt ataaatgttg
     4441 agtcacctgg atttaatcta actacctttt cacccataca aatatcaacc acaccactcg
     4501 ttaccaccat aatttcttct gatgcatgat atatataatc tttggcatcc tcactttctg
     4561 gtttaagttc aaactctaca aataaaattg aaggtgtaaa ctcagttgta ggcattggag
     4621 atactatctc gtaaaacatt tcactctttg gaaatttcat cataactcta tcttcttttc
     4681 taatgactaa atcatcagta ttattgtcat ccataaacat atataaagga gtgtctaaaa
     4741 cttcagcgat ttttctcaat gaagacaagg atggttcaat agcatctctt tctatttgag
     4801 agatataact tatagataaa tctgtcaatt tggaaacatc acttaatgtt tttttcttgc
     4861 tttttcttat atttctcatc ttttcaccta gcataagaac ctccaaataa taaaattaat
     4921 ataaaatatt cgcttaaaac aaagtaatta tagcacaaca tagaagtagt ggcaataaga
     4981 atacatagta gattttgaca attaaaattt aattaaatcc aaataaaagt ctatgaaaat
     5041 gaattattgt taaattaata caaagaaaat aataataaaa aaatttaata gaagagtatt
     5101 aattgaatta agattatcac attatatttt ggaaaagagt aaattttcag taaattatct
     5161 tgacatatat attgaagtaa taatataatt taaagtataa aattttaaaa attgaattat
     5221 aacttcaaaa aataaggagg gtaatatgaa gataacagat atcaaatttg aaaagcttag
     5281 aattaaatta aaaaaacctg tagtagtttc atttggagta attgagtatg gagaaagtat
     5341 aattttaaaa attgaaactg atgaagggta ttgtggattt ggcgaagcag cacctcttgc
     5401 agcagttaca ggtgaagttt tggataatgt tctttcagta ttgcttatgt ttaaaaaaga
     5461 gttaataggc aaagacccac tagatattga aaccatacat accatcatgg atggaattat
     5521 tataggtaat acttctgcaa aagcagctat tgacatagct ttatatgata ttaaaggaaa
     5581 aattatgaat gcacctcttt ataaagttct tggaggattt gatagtaaag tccaaacgga
     5641 tattactata agtatagata agccagaaaa aatggcagta gaagctttag aaagagtaaa
     5701 agaaggtttt aggatattga agcttaaagc aggtataaat cctgaagacg atattgaagc
     5761 tgtaaagtta attagaaaag ctgttggaga gagtataaga attagaattg atgcaaatca
     5821 aggatggaac gtaaatagca gtataaatac tatcaaaaag ttagaagaat ttaatgttga
     5881 tgcaattgaa caagcacttc cacattggga tttagatggt actgcttaca taagaaataa
     5941 aagtaataca aaaattatga tagatgaatc attacattca ccaatagatg caattaaggc
     6001 tataaaaaag aatgcagttg atacatttaa tattaagctg atgaaatcaa gtggaatata
     6061 tcctgctata aaaatcaaca acatagctga ggcaagtgga gtaaattgta tgttaggatg
     6121 tatgctggaa acgagaattg gtataacagc agcagcaaat ttaatagcat ctaaaaagaa
     6181 cataacagaa gctgaccttg atagttttat gttttgtgaa gaattagaag gtatatctgg
     6241 aggatttgta atggatggag atattatgaa tcttgtcaat aaaccaggtt taggaataga
     6301 agtaaactta tgataaattt ggaggttaaa tatgaataca cttttttcaa atccaggtag
     6361 tatacttgct gtaatgacat caatgattgc attgggtttt tacttacaaa gatacaaagc
     6421 cattaaaagc ttaggaccag cacttacaat aatcataatg ggaataatac tttcaaactt
     6481 aaaagtagtt ccagtaagta ctgagttata tggaactatt tcaacatatg ctattccagt
     6541 ttctatgact ataatgttga tgagtgttga cttaaaagaa atgacaaaat tatcaagaga
     6601 acctttaatt gcaatttttg tagctgtatt aactgtaagt atcatggcat ttttatttgg
     6661 attagtattt gcagaaaaaa tatcagaagg ttggaaggtt gctggaatgt ttgtaggtac
     6721 atacactggt ggaagtgcta atcttacagc tattggaaca ggtcttaatg taagtagaca
     6781 aactcttgca gcagcaaatg cagcagacta tgtaattgga gtaccaacat tgatatttat
     6841 gtttgctcta ccagcaatat taaaaaattc aaagaagttt aaaaaacttt ggccatatca
     6901 tgtagaagaa tcagaattag aagattgtca aaatgaagaa tttatggagt caaaagaatg
     6961 gagtattaaa gatatagctt ggatgttggc tataggtttt gttgttacag aagtttcaac
     7021 aatacttgct ggatatttca attcaagttt tagtagtgca gcaagaatac ttttagttac
     7081 aacaatttca attataattg ctcaattaaa gccagtaaag aaactaaagg gaaatttaga
     7141 tttaggatta tttgttgcat tattcttttt atgtacaata ggtttttcag tagatataaa
     7201 agagttttta ggttcaactt ttactataac actttactgt tttagcataa tttttgcttc
     7261 atttgttttc catttaggca taactagact tttaaagata aagtatcaat atgtaatact
     7321 ttctatagta ggagcaattg ctgatggtcc tacttcagct ttagtagctg cttctgcaaa
     7381 gtggaattcg cttgtaagtg tagcagtcgt aatgggagta attggtggag tattaggaaa
     7441 ttatgcaggt atatcagtgg catatgcaat aaagatgtct ctaggattat aggggggagt
     7501 ttaattgata aaatttaaat tatacatatc tggactatac agtggaaatg taatctttga
     7561 tggagattta ttaattgaaa agttgaatcc ttttacaaat aaaatagaaa gtttaaaacc
     7621 aataagcaaa gaagaaaata catactattt aaatcttaca aaaatagatt taaagtcttt
     7681 atttaataac tttgatgtat atactaaaag cttagtaaac acagataaag acacagttat
     7741 aaataactta ggagaaacgc attctaaact taatgaatat atatgggttc aaagaaacaa
     7801 aaagttccca ctagatataa ttattgtaga taataaaata gtaggtttta tctgtttatc
     7861 cagagaaact tgtactatat taattatgga tggatatgaa gagtatactg tattaaaaga
     7921 atgggaaaaa actcataaaa atgaagagat atattctata agatttggtg gaaattatat
     7981 gattgatatg aaagatggta taaaactttc tacagatgta tatctccctg attttgtaga
     8041 ttctaccaaa aaagcaccga caattcttat gagaactcca tatggaaaag aaaatgataa
     8101 agaaatatat tataaatatg tacaaagagg atatgctgtt gtaatacaag atgttagggg
     8161 aagaaatgaa tcagaaggta agtgggaacc tatgattcat gagagggaag atggagatag
     8221 taccataaat tggatagtat cacaggaatg gtcaagtgga atagttggaa tgcttggagc
     8281 atcatatctt ggttatgtcc aatgggcagc tgcatcttca ggcaataagc atcttaaagc
     8341 tttagttagt atagtaacat caggaagccc ttttattgat ataccaagaa aaggaggagc
     8401 ttttgtatct ggtatgcttg catgggcatt tatggtatca agaaataaag ttgatagaag
     8461 caaaatggta agagatgatt gggatgatgt tttaaacata cgaccaatag aaaatatacc
     8521 ttttgaagct cttggatata gaattgaatt tctggaagaa tggttaaaga gagtagaaaa
     8581 agatgagtat tgggatttga tggattggca tttacaaaaa gataaaataa atgtacctgc
     8641 attggttgta tctgggtggt atgatgataa tagtatggga actacagagg ccttagatgt
     8701 tataaaggac tatgaaaaag gaaagagaaa ggctattcta ggtccatgga tgcataattc
     8761 aaatacttta agagatataa atggtatttc tttaggaaat agttcattaa gatatgattt
     8821 ggattataat tatctattat ggtttgataa atacttaaaa ggtatagaaa ataacataga
     8881 tacgactgct ccagtagagt attactctgt aggttctaat aagtggaaga cagaagaaaa
     8941 ttggccaata ataaataaga tagataaaag tatgtatcta ataagtgatg gaaatgctaa
     9001 cacatcttta ggaaatggta gattagtatt tgataatgat ttagaagaaa agtatgatag
     9061 ttatatatat aatccaaaag acccatcagt acaactaatt gatatgtcag agaatgaagt
     9121 tggagttcca aataactaca aagatttaga aaaaagaagt gatatgttgt gttatacatc
     9181 agatgctttt agtgaagaat ttactgtaac aggtgatata aagttagagt tttttgcatc
     9241 aagttctgcc aaagatactg actgggttat aaaaattatg gacgtggatt tagatggaaa
     9301 ttcaattaaa ttagctgatg gtatacttag tgctagattt agaaatagtt tttataagtc
     9361 agagtttatg gaagaaggag agatttataa gtttacgatt ataacttcaa aaatatcaaa
     9421 cacgtttaaa gttggtcata aaattagact agatataacg tctagtgcta aaaactttat
     9481 tttccaaaat agcaacacga cagagggata taatagtatt gaatatatag aagcaaagaa
     9541 tacgatttac catggaggta aatatccatc taaattgata ttgccaatag agaataagta
     9601 aatttatata gattaaatag tgaggaggtg tctcaaaatg aattttttag ttcgtgagac
     9661 actttctttt atacgaaatc aaatatattt tcataatttc aacaatgaaa aagaggctta
     9721 tctctctgtt ttgagatagc ctcctataag ctaaattgat tattttgtag ctgcaacaac
     9781 gctcatttca actaacaaat gctctctagc aagtctagct tcaacacaag ctctagaagg
     9841 ttcaaatcct ttttcaaccc aagcatccca aacagagttc atagcttcaa agtctttcat
     9901 atctcttaaa taaatagtaa cagatagcaa atgttgctta tctgaaccat atttgtttaa
     9961 taaatcttct attttagcta aaacttcagt tgtttgttca attactcctc cttcagcatg
    10021 agtttgtcca cataaataaa ctgtattgtt atgaactaca gctctactca ttcttccagt
    10081 tccttcatat ctctttattt ccatatttta aatacctcca tagttaaaaa atctattaaa
    10141 aatctaaata ataaattaaa aaaagtctat actatataaa attatattat atattctgat
    10201 aataatccat aaaaacatat aactttttag ctataattat tattgttatt aattgaaaag
    10261 atttattagt tacttgacat agtttacact tttaattata gtataaattg gaatatttag
    10321 ataattatta tttaatgtat aagctaagtt tttaattaat atggctttat acatttatat
    10381 agaaaagagg tacgtatgag tagcacaatc aaatcaaatc gaataaaagg tataatgttt
    10441 ataatagcat cagcgcttgg ctttgcaatg atgtctgcat tcgtaaagct agctggagat
    10501 ttaccaagtt ttcaaaaagt gttctttaga aatctggtat cagctataat agctttttgg
    10561 cttatcataa aacataaagg aagtctaact ggtaaaaaag aaaatttaaa agtactttta
    10621 tataggtcaa tatttggaac tctaggagtt atatttaact actatgcaat tgacaagttg
    10681 atgctttcag atgctaatat gctaaataaa atgagtccct tccttgtagt aatattttgt
    10741 gcaattgctc taaaagaaaa aataaatgta aagcagatag gagctattat aattgcattt
    10801 ataggagctt tattcataat aaaaccaaca tttagtgtag aagtaattcc atatcttggg
    10861 ggaattgcag gagctgtatt tgcagcaatg gcatatactt gtttaagagt tcttggagat
    10921 agagaagatt actatacaat tgtatttttc ttttcagttt tttcattagt tactgtaggg
    10981 ccaattgctt ttgcagtata tgaaccaatg acccttatgc aacttatata tctattacta
    11041 ggtggagtat ttgcaagtct aggtcaattt ggaataactc tagcatataa atatgcacct
    11101 gctaaggaaa tctctatatt tgactatagt aatataatct tttcagcaat tttaagtata
    11161 ttcttattca atgtctatcc agatatgtta agtgttgttg gatatttaat agtattttcg
    11221 gcagcatttt atatgttttt atacaataag aagttagata aattagacaa agaaaagaag
    11281 aagtaaacta gataaaagtg tttttataat ttgtattgaa tatttaagtc tttgtattaa
    11341 aattaaagaa aatttaatta aaaatgtttg gtatattaac ttataacagc agttaaaatt
    11401 gaaaaattat aactgctgtt tcttttatta aaaatatcca aatgtctgta aaaatagact
    11461 ataatagagt agtataaata atataaaggg gataaattaa agtatggaaa aagtgaaagt
    11521 tgctgtgatt ggagcaggta atagaggaac gtatgcatat gctccatata tttatgaaaa
    11581 ttctgatgta tgtgaaatag ttgctgtagc agaacctaaa aaaggaagac gagaactatt
    11641 tacacaaaaa tataacctag atagtaaaaa tgtatttgaa actttagaag acttttttaa
    11701 acatgacaag atggcagatg cagtaataat agctacaaat gatgatagac attatgatgt
    11761 agcaaaacta gctcttgaaa aaggttatca tgtactatta gaaaagccaa tgtctaatag
    11821 tttagatggt ctggtacata tagatgactt gtgtgataag tataaagata agatttttat
    11881 gatttgccat gtactgagat attctccatt ttataataaa ttaaaagaaa ttgtagaaag
    11941 caaaaaatta ggagagctag ttagtataca atataatgaa aatataggat attggcattt
    12001 tgcacatagc tttactagag ggaattggag aaatagcaat gaaaccagcc ctttaatttt
    12061 agcaaagagt tgtcatgaca tggatatttt gttatattta gtaggaagta gatgcaaaaa
    12121 aatatctgca tttggaagct taaagcatct taataaccaa aatgctagtg gagagatggc
    12181 acaaaattgt cttcagtgtt tagttgaaaa aaaatgtcca tattcagcaa agagaattta
    12241 tttagaaaaa gatagaagta taaatagagc tgttcacata aatccaactg aagagaatct
    12301 attaaatata cttaagacaa gtccatatgg aagatgtgtt tacagatgtg ataataatgt
    12361 tgtagacaat atggtaaata tactacagtt tgaaaatgga gtgacggcta cttttaattt
    12421 atgtgcattt actaaagaaa atggaagaac tataaagtta atgtttagcc atggtgaagt
    12481 tggtggggat ttaaataaaa atgagataag aattaaagaa tttggaaaaa acgaagaaat
    12541 agttatgaat ccatcaaatc aacagaatat ggtagaggaa tatgatagaa atcttatagc
    12601 tgaatttatt aaattagttt caaataaaga actagaaaag ggtagagtag cagcaaaaga
    12661 agctatacaa agtcatgtaa tggcatttgc agcagaatat tctagggtca gtgatgaagt
    12721 agtgtatata gaagaatttt ttgatagtgc taagcaaatg actaaggaga tagaagaaac
    12781 tatattttaa ttaataaaaa tttattaaat aaaataaaga tagattaaat aatagaaata
    12841 cgaaaatatg tgttctatta aggaatctat cttttttatt aaggtttttt cttatcccta
    12901 tcaaaaaaat ccatacattc tgataaaaga taagaaagta aagcgaataa gatttctgta
    12961 cttaaaggaa tcgttactga atccataaag gtatcaatta tgttcatagc tagaaaagtt
    13021 agtataaaat caattataaa ttcgcaaagt ttatatattg agtatggtaa tttgtttaca
    13081 tatctgaata tatctaaaat agtagtgcat agaaaatcaa ttggagtggt tatacaaaaa
    13141 tatattaaaa agaaaattaa tacagctcca agagatttat attcaaatcc taagaatgat
    13201 aagaagccaa ttcctataac cgtgaaaata caaaaagtta taccaaaaaa aacagcaaaa
    13261 aataaaaata cagaaataaa ttcgcctaaa ggaatccttc ttttattact tatcttaatc
    13321 aactccttaa tttatgatat gaaatttata taaataataa cattcttaaa aattcataat
    13381 agtcctctgt ttcaaatagt actgtttgag aatctatttt tttcatattt ggataaaaag
    13441 aggccttaaa agctttataa tgttctctaa attttatttc tatcttaaat ttatctggca
    13501 attttattaa gtgcctagat aaatctccag ataaagcttt tttgacacct ttttcaattt
    13561 tttttagtgc aaggtttgga tggattgaaa ttgaaccatt tccaattcct tttgacacag
    13621 gaacagtgac tatatttgga tttagttttt ttgcactctc acaaagcatt tcatctccac
    13681 ttaaaaatgc tacaggaaca ttataatatg cagcaatata agcatttata gtaaattcag
    13741 atgctatttc atcatttatt ttaaaatagt cataatcaat atccatagta tgagaaagtg
    13801 gattaccatt acaagtagct ccagagtgat agccggtgaa tatacatgca tcaaagcttt
    13861 catcaattcc tgccatcata actagtgggt ctttggtcca tcctctaagt attttgacgt
    13921 tttcaggtaa aatatttgga ttaatatttc taccactgtc atgtgcatct ttaacaagta
    13981 tttcatgtac accagattga gtagctccat cacatgctgc tgttatctct ttactcattt
    14041 gtgttctata gtgttgcgaa aattcacatt caagttttgt ttcatcccaa tttacaatac
    14101 cacaagtacc ttctatatca gcacttaaat acaatttcat aatatatacc cccttatgtt
    14161 tattattgat gataataaca gataacagaa aatacataat caataaaggg cataaaaata
    14221 ttatgtctta ctattaacta ttattaaatc tgtaactgaa aaataactaa attggtataa
    14281 aaaaatataa cactatgtgt catttattta tatagttttt tatatttgat gtttatataa
    14341 aattgtaaca tataagtggt tacatttact agtttaacat agttaaaata gttaggtatt
    14401 atttttaagt taaattgatt attataaaat cgcagaataa aagaaaaatt taatttatta
    14461 aaatgcatga aatgtttttt cctcaacata atgctaaatt aaactcaaaa agaaataata
    14521 gaaaaatctt atgtagctta gaaagaatgt gaatgattta agggaaaaat agaaatttat
    14581 tatataatat taggttgtat tatataatac agcctaatat tatatgtata tgtgacataa
    14641 aatcttggta agtgtaaata aaataaatgt ggaaaacaag ttaatgaaat caagaaaaaa
    14701 gtaatttaaa attcaatttc atcttcccaa ttaaattctc ctgatttttc tacactctta
    14761 ggccagtgac tacaccattt aggaatggac ggattttcaa ccctatcaac gcttggagta
    14821 tatggcttta agtctataac aggactatta tcattagcat caatatatgg tatttttatt
    14881 ataccatttt catggtcgat attgataatt tgtacagcag tcaatgctat tggattgggt
    14941 cttataggtg accttgttgc aaatattcct ataacatctg gtccactttt ataaggtttt
    15001 ggggtttcta aaatacttct tagttcagga ttatcaaacc catcagccca ccaaaataca
    15061 tttaaatgac tgaaatcgtc gagttcattt aatgcaggta catacttttt gtctaattta
    15121 ataaagacct cttcgccttg aatacatatt ttacctatag gatttattaa tatttctttc
    15181 ataatattac ctccaactat ttaatttatc atttgtaatt ttagggtata acatgacatc
    15241 atgtgagggt caatataaaa taataaataa aaatattaat agttagaatg ataatacatt
    15301 ttttacatgg agtatgacac cagtaataag gcaatcttcg tcaatatcaa aagtagaggt
    15361 atgcaaaggg gaagtaatgt ttttttcacg atttgcacaa ccaaggtggt aaaaagctcc
    15421 ttcacagtgt cttagaaaat acgaaaagtc ttcagcccca agagtgggcg agtctttaag
    15481 gataatattt tcttctccaa acaattctac agcattttgt ttgacgtaat ctactagatg
    15541 gtttgaattg ataagagctg gataaccttc ctcaatttca acagaaatac ttcctccaaa
    15601 tgttttacaa gtaaaatcgc aaatttctct aattcgtttt tttgaaaact ctctagtttc
    15661 aggagtcaag gttcttaaag taccccttat aaccactttg tcacaaacta tattttcttt
    15721 gattccacct tcaatctttc ctagagaaat aaccacagaa tcagttggat ttgtgtttcg
    15781 actaacgatt gtctgtagag ctgtaataat atgagcagat ataacaatag catcaatacc
    15841 ttgatgtgga taagctccat gacattttga accatgtaca gttaattgaa gtgtatcggt
    15901 ggaagcattt agagtgttgt attttaattc aattagattt ttgttgatat tgggatttac
    15961 atgaagtcca aatatatatt ttacatttgg gttttcaaga catccatctt ctatcataag
    16021 ttgagcccca ccaatagttt cctcggcagg ttgaaaaaag aattttacat ttacatttaa
    16081 gtaatccttt aatttaaata gtatattaca agtacccagt aaaatagcag tgtgtgcatc
    16141 atgaccacat gcatgcattt tgccaatatt aatagattta taagaataat taagttcttc
    16201 tgttataggt aaggcatcta tatcagctct tatagcaact gtttcaaagt ttggactcac
    16261 gttaatgtat gcagttatcc ctgtatgatt cttatattca atataattta tacctatttc
    16321 atttaagtat ttaataattt ttttcttagt ttgaaattct tctaatccaa gttctggagt
    16381 cttatgtaaa tctcttctta tatttataac ccaatcattt atctcatgac aaagagtttt
    16441 tatattaagc ataaaacaca tcctaactat attaaaaatt tatttttctt cattaattat
    16501 attattttat gtaaatcatt attatgtatc aagtacaaat aaaatatctt ataattatat
    16561 tagagatatt tagtatggag aagaatatat cattttataa gaagaggtgt ttaaatgtta
    16621 ggaataattg cgccatctgg gccattaaga aatacaagtt tggaggaaat aaagtttaat
    16681 ttagaaagtt atggttacga agttaagttt tctgaaagtt gtagtttaaa ttataaaggt
    16741 tatttagctg gtaatgatga tattagagca agggatatag aagatatgtt tttagataaa
    16801 gatgtagata taattatgtg cttaagagga ggttatggaa caacaagaat attggataaa
    16861 ataaattatg atataataaa gcaaaatcca aaaccattta taggtttttc tgatataaca
    16921 ggtttaaatt tagctttcta taaaaattgt ggtcttttac catatcatgg aataatggca
    16981 gcaaatgtag gtaaatggga taaatttacc tataaatcat tggtaaatgc tttagaattt
    17041 aaagatgaat tatatttaga aaatccaaaa gaagaaaaga tttatactgt atgtgaaggt
    17101 aaagcagagg gaataataat gggaggaaat ttatccttaa taattgccac aatgggaaca
    17161 aaatatgaaa tagatgcaaa agataaaatt ttgtttattg aggaaattgg agaacctcaa
    17221 tataaactgg atagaatgtt aacacaacta tattcttcag gaaaacttga ggaatgtaat
    17281 ggtataattt ttggagattt taaggattgt attgaagaaa atgatttgat ggaattacta
    17341 atagaatttg caaataaggt caataaacct tctatatata atcttcagtc aggacattgt
    17401 atcccaatga taactattcc tttgggcaga atgtgcgaat tagatgctac agaaaaaatc
    17461 gttaaattaa aaaaatagtg tgtatataaa tataatagaa atatggtata atcttataca
    17521 agtaaaaatt tagctaggag gatgaaaaat gtctgatgat agaataatag actttaacga
    17581 actaaagaac aaagttaaag attctgatgt agataaattt gagcaataca tatataactt
    17641 atatttttct gttatggatg gaaagatgtc tatggctgag ttttcaagaa aaatattcga
    17701 ttatatgaga gataacaaca tatctcaaga aaaatttatg aaaatacaaa agcaatttat
    17761 ggagagatat ggtatggaca ctgaagaagt agaaaaacaa cttagaaact ttggaatcga
    17821 cccttctaca gcaggtttta tgagtaataa tacttccagc aaagtatcta cagaagattt
    17881 agagtcattt aagaagagtg caggatttta tgagaaatat ggagaaaaaa tccaaccaaa
    17941 gagttgtata acaacattta taaaaaatga cttaaatgat attaatgtaa taatcgacca
    18001 agaaaagata atgctttgta gtgatagaaa aataaatctt atggattcag aactaaatga
    18061 gtttttatta gaatataaga atatgtttaa taaaaaaata aaggtagtaa tgtgcgaaac
    18121 tacgaataag tatgattatt aaaaaatgaa tttaaatact gaagtagtat caattaattg
    18181 tactacttaa aaataaaaat cgcaatttat tattccttat aaatttgtat aaggaatatc
    18241 taaactgcga ttttttatat tgtgaagatt agtcataata gatagtgttt taaattgtaa
    18301 tcttctcact aaaactgtgt ttttctatta gaaattttat taaaaataaa tatctgataa
    18361 ttgtattata tggatgtaaa gtattagagg cttaacgaat ctcttttaat acattaactc
    18421 cattttgaat catactgttg aaaaatacta agtttaaaat ctcagcaggg tgaacgttag
    18481 gaatatcata agtatctact aaattcatag gtacaactat atttttttca atattatgtt
    18541 gattaaaata tgattttaga gttattgcaa attgatatat acatatatct gtgcaatcgc
    18601 caactataat tatattatct atattatcaa aatccaaatt ttctaatgca aaaaatccat
    18661 tagtagaatt tttaggaaga atttgtaggt tttctatcga cttaagttca tcaaccaatt
    18721 cactttctac atcattttcc aaacagtgaa caggataact caaaagttct attgatttag
    18781 gagtatgaga atcagtaaat gctattactt tatttaattt gtttgatatt ttttttgtaa
    18841 acatttcaat tggctttatt aaagactcta ctctaggaga gtataatgca ccttgtcttg
    18901 caaagccatt attaacatca attataaata aagcagtttt actcaaatca taattttcta
    18961 ttttttctat tggtagatta tctaaattac tttttaaagc ttcaagttca tttaataagt
    19021 tatccatagt tatacctcca agataaattt aataaaattg tacatattaa ttatataaaa
    19081 gtcaaggagt gcaaactatt gtataattag ctgtattgtt ttgagtgtaa aagatttgaa
    19141 gcaatatctg gatagttagt aaaaatacca tctacattga gttttaggca gtgtagtata
    19201 tcatcctctt catttacagt atatacattg acaccaagta gatttttgtg acaaagttca
    19261 attagagatt catttactaa aactagaggt ggatgaaggt aactattacc cagaaattta
    19321 gcaaaattga ctacattgtt tataggtaaa taacacaata atcctgtttt tacactaggg
    19381 tctaatttat gaaagtttgt taagctattg ctgttgaaac tagatataag aactctattt
    19441 ttcatattat actttaaaat aagctctaaa acgtcttttt ctatattggg atagtttatt
    19501 ttgtcagttt ttagttctat atttaaaata aggtctgtag gtgaaattag aattaatact
    19561 tcttctaaag ttggaatttt acattcttta ttgctttgat atccttcaaa agatgagttt
    19621 agagttttta attttcttaa agtaaagtct tttacaaaac catgtccatt aaaagttcta
    19681 tcaacctttt catcatgaat aactacaatt tttccatcct tagttctgtg gacatcaagt
    19741 tctattccat atatattcat atccaaacat tttttaaaag ctagaatagt attttctgga
    19801 tactttccac taaaccccct atgagcataa atattcattt atatggttcc ctcctataag
    19861 tataacttat cttataaaaa tttaattaat ataatatatc atattccaaa aggtattgtt
    19921 atatattagt taaataacta tttgtaattt atatattata taaatgtaga aaaataaaat
    19981 catatatgtg ttataatata ttttaaacat tgtaaatatt acgaatttag tagtcataat
    20041 aagtgtaatt ttatgcaagc tcatgttttt agagcttgca tatgtattat taatatattt
    20101 atatattgtg aaaataaaat tccaaaagaa ttttttctat agacttagta gtaaggggga
    20161 attttgatat gtatgaagtt aatttagaac gagctatgaa ttattggatg gatttgttag
    20221 atgattatac gaatatagca aaaattccaa atgatttttt ttataaagat tgttctgatg
    20281 aagtagtaga tgaggttttt aagatgaata ttgagaaaca tgaaaggtta attcaatatt
    20341 ctaaagtgaa caatattagc atagactcta tcatagaaac tatatatggc cttatacttc
    20401 aaaagtatat atatgaagat gatgtagttt ttggtaaaac aatgaatata attccaataa
    20461 gagtaagagt tagacaaagt gaaaaattta tagatgttgt aaggaagcta aaaaggcagt
    20521 ggagcaggtc tttagagtat ggatactatt tgttatcaga tatagagaat aaaaatcatc
    20581 taaatactaa gcttataaac acaatattta tagctaaaag ttttgattta tcatatgatt
    20641 atgacataga aaaaaaagga tatgatttat ctgtaatagt atatactaag agggcactta
    20701 aaataaaaat aaagtataaa ccatctatat ataaaagaga aactattaga agaattttaa
    20761 atcaatttga ttatataata gagcaaataa tagaagataa tgatatttta atagaagatt
    20821 tagaatttgt atgtaaggaa gaagaaaaag tattattgga agactttaat aataatcaat
    20881 atgaaatacc ctatgataaa agttatgttg atttatttag ggagcaagtt aaggtaacac
    20941 ctagtaatat agctgctagg gatgaaaata gaaagttaac atataaagaa ttggatgaag
    21001 taactgataa acttgcaggt tatttaaatc atataggagt ggaaagtgaa gacatagtag
    21061 ctgtaatgtt accaagagat attaacataa ttattacagc aatagggatt atgaaatcag
    21121 ggggagcatt ttttccaata gacacaagta atccagaaga aagattgaat taccttcttg
    21181 aagatagtaa tgcaaaagta gtgataacta cagatgaact caaaagtaag gttgtaaatg
    21241 agaataccat agtactcgat ataaatgatg aagaaatgtt taagcttgga tatgaattaa
    21301 ctgaaaaaat aacatcatca aattgtgcat atacgatatc tacttcaggg tcaactggta
    21361 ggccaaagac catagctata gaacataaga gtttagtaaa tatgtgttat tatagcgtaa
    21421 aatctataag cgctacagaa aatgatatat gtggtatata cttaagtttc agttttgatg
    21481 ctgtaatgaa acaactattc ccttatttgc ttgtaggtgc atcaatagat attatgcctg
    21541 aagaagctaa atttgatgaa tatacagtaa atgaatactg tgaggaaaat gacataacta
    21601 tactagcatt gccaacagca tttgcaaggt tatttataca aaattgcaat aataattcgc
    21661 ttagagttgt acagactgga ggagaaagat taaaaggata tagaaagcgt aattatgaac
    21721 tatataatga gtatggacca accgaattta ctgttgtatc gacttctttt catgtggaca
    21781 gagaatatgg aaaaatacca ataggaaaac ctatatttaa tacttatgca tatgtattag
    21841 ataaaaagaa caaactatgt ccaattggag caccaggaga gctatgtcta tcaggcattc
    21901 aaatatcaag aggatattta aataaaaaag gtctaacaga gcaagtattt gtagagaatc
    21961 cttacaaaac ttgtgaacat aataagttaa tgtatagaac tggtgattta gtaagatggt
    22021 tagatgatgg aaatttagat tatattggaa ggatggacaa tcaagtaaaa attgatgagt
    22081 ttaggataga gctatatgag attgaaaata taataaataa tattacagaa attaaaagtg
    22141 ttgtttgtat atcgagaaca aatgatgatg gagatatgta tatatgtgca tattatgtaa
    22201 ttgatgagga agatagtggt aagataaatg aaagaactat tagagaatat ttaaatgagc
    22261 atctacctcc atatatgatt ccaacaataa taatgaggat agataagata ccagttacac
    22321 caatcggaaa agtaaataaa agagcattac cagaataatt ttttattatg ataggtgtca
    22381 taaagtgatt tcagacattt ctttatgaca cttattttta taaaagaatc aatttgcata
    22441 aaaacaagaa ttttctgtat atatttacta tggtgaaaat ttatttataa gaatgattat
    22501 tgtatttttt atacttttcc gtgttttaaa gcattttttt tacaaaatta attttacatt
    22561 aaaaaaattt tttattttat atcttaatag tttttagtag aattgaaaaa taaaaagaaa
    22621 attagcataa aaatatctaa aagtagaaat taataatata gggttaaatt ttagtagttt
    22681 tgactcgcta tttgtaaaat aataaaaagt taagttttat atgtatatat gttataatat
    22741 atacaaattg aaaaggggtg aaaattttat gaacaaaaaa ttgcctaagg gtgcttatgg
    22801 tgaggttagt ggaaaagatt acgttccata tatcacagat aagtccagaa ccggtggaaa
    22861 tgtagctgta ctgattatcg gtattatttt agctgccatt tttgccgcat ctacaactta
    22921 ctctggaatg aaagccggtc ttacagttgc cgcaggtatt ccaggagcta taataggttc
    22981 tgcttttgtt ggagcatttg ctcgttcaaa gggtattctt ggtaaaaact taatccaagg
    23041 tatgtcaagt ggtggagaat ctgttgcaag tggatttatt ttcgtattac cagcagttat
    23101 attaattgga agccaaatta cattctttga aggcttagct gttggagttg gtggagtttt
    23161 atttggtata ggaattgctg ctatagttca caactatcta atagttgaag aacatggtaa
    23221 acttatgtac ccagaatcaa tggctatatc tgaaacactt gttgcttcag aggctggtgg
    23281 agattcaatt aagtacatgg gaattggatt tgtaataagt ggatttatta ctgtattaac
    23341 tggttcattc ttaaatgtag caaacaatgt aatgagtctt gtaggaagca aattctacaa
    23401 atggaaattt gatattgaag taaatcctct acttttagga attggattta tagtagggct
    23461 tgaagtttct ttaacaatgt ttgcaggaag tattttatca aactttggta tagcaccttt
    23521 aataggatac tttacagata tggctaaaga tggagctatg gtatggaata atcctgcaat
    23581 gcctcttaac caaatggatg ttggagcaat atctagtagt tatgtaaaat atataggtgc
    23641 tggtatgatg ctttgtggag gtattattgg tgcaataaaa cttataccaa ctataatcgc
    23701 ttctataaaa gaaacactta aagctaaatc aagtgctgga gaaggagaag aaggttcatc
    23761 tatacaaatg atacttttat taggtggagt agttattgga tttttagcag cattcttaat
    23821 ttctggtaac attgttatgg caattattgg agctatcata tctcttttat tatctttatt
    23881 atttgtcata gtagctggtc gtttaacagg aacaatcgga acatcaaatc ttccagtttc
    23941 tggtatgact atagcttctc tagttattgt aacattagtg tttgttataa tgggatggac
    24001 tgatttagaa gctaataaat cattactttt atttggttca tttatagttg ttgctattgc
    24061 tatagctgga ggttatactc aatctcaaaa agtaacttat attattggtg gtagcaaaaa
    24121 cgaaatgcaa cgttacttta caattgcaag tatagttggt gttatagttg ttgtaggagt
    24181 aattttatta ctttctgacc aacttagagc aactggtgat aatgtacaat ttgcattacc
    24241 acaagcaaac ttaatgtcaa cattaacttc tggtattatg tcaggtagtt taccttgggt
    24301 tatgattatt gttggagtat ttatggcaat cgttctatat gcattaaatt taccaataat
    24361 gactatagct ataggattct atttaccaat tgcaacaact tctataatat tagttggagc
    24421 attaattcgt ttatttgttg aattagtatc taaaactgaa aaagaaaaag aagttaaagt
    24481 ttctaatggt ataagtttat cttctggtct tgttgctggt ggttctatca ttggtttaat
    24541 aggtataata ttacaagtaa ctggagttgt aactccaaag gtaccaagtg gatttgctgc
    24601 aacaaattca atggcaattg ctttattggt agttttagtt gtgcttacag cattacctat
    24661 tgttttatct aaagtaaaaa ataatgagca agagtaatga tgttttagat attgtattta
    24721 aaattactgt tggacttgaa tatgaagtag ctgaaaatag tgttgttact atacttgaaa
    24781 aacaggacca taaaattcaa aagtttttta gaaagttaaa agctagaata cctaaataca
    24841 aaaaaataga actagatgaa tattccagtt ttgttttttt acaaattgat ggtcaaagga
    24901 ctgttagaga aataggcaag aacctagaag ctaaatatgg agaagaatct catccacttt
    24961 atgaaaggtt gttagtattt ttaaatcata ttgaagttaa ttgtcactat attgaaagac
    25021 tggatatata gatataataa gaattattat agatatatta gagcatataa attagaaggt
    25081 aattatgaaa gaggctgtct caaaatagag ataagcctct tttgtattgt tgaaatgatg
    25141 aaaacatatt tgatttctca taaaaaagag tgtctcataa aactaaaaag ttcattttga
    25201 gacacttcct ttaaaatgtt tataaagtac agatttaaag taaaacttat taaacttaag
    25261 attattttaa tatcacttat tattagctat ttcatcagct agattattta aatattccca
    25321 tctttcatat ttgtattcaa gttctttttc tagtttagat ttctcatcta aaatttcttg
    25381 aagtttagta aaatcagttg aaaattttgc agtactttca tcaagtgttg caattttaga
    25441 ttcgagtttt tctatatcag agtcaatatt ttcaaactct ctttgttcat tatatgagaa
    25501 cttaagcttt ttatcatttt taggtttttc tttttttagg gtagtagagt catctttttt
    25561 aactgattcc tttttagatt cttcaaattc aattccttga atctctctat aaataagata
    25621 atcactgtaa ttacctgtat agatatgaat ttttccatta ccttcatatg caaatatttt
    25681 attacaaatt ctatctaaga aatatctatc atgtgatact gtaactacta cacctttaaa
    25741 ttcgtctagg tattcttcta atattttaag cgtttcaata tctaaatcat ttgtaggttc
    25801 atctaaaaga agcacattag gagctgtcat aaggatttta agaagatgta gtcttcttct
    25861 ttcaccacca gataatttac ctatcaaagt atattgcata gtaccattaa aaaggaatct
    25921 ttcacacatt tgagaagctg taatcctagt accatctgaa gtttctatat agtcactagc
    25981 ttcttttaca tagtctatag ccctcatatc aatattcata tgagagtcgt cttgagaaaa
    26041 acaacctatt ttaaccgttt ctccaattga aatatatcca ctgtcaggct ctatttctcc
    26101 attaagtata tttataaggg tagatttacc cataccattt ttgcctatta taccaattct
    26161 atctgtacga gttaaagtgt attctaaatt atctataaca gtattttctt caaatgattt
    26221 agatatattg tgtatttcta ttattttatt tccaagtcta cttgatgcaa cagatatatc
    26281 gagattttca tcaggtttaa aagaatctct atttataagt tcatcaaatc tttgtaaacg
    26341 agctttttgt ttagtgctac gagcctgagc tcctcgtctt acccacgcaa gctctgttct
    26401 tattaaattt tgtcttttat cttcaatact tgcttctagc gaaagtcttt ccattttctt
    26461 ttccaaaaat attgaataat tcccatcata actgaataat ctacctttgt ccagttcaat
    26521 aattctattt gtaacacggt ctaaaaagta tctatcatga gttatcataa gcaatgagcc
    26581 ttttctggaa ttaagatatt cctcaagcca atctatagta tcattgtcta gatggtttgt
    26641 aggctcatct agtatcaaaa actcacaagg tgtaataagg gctgatgcca gagagactct
    26701 ctttctttgc ccaccagaaa gttctgctat tttttgatta aagtcattta taccaagctt
    26761 tgtaagtact gacttagctt cactttctaa atcccaaaga tttaaggcat ctattttttc
    26821 gtgtactgat aataattttt tatttaattc ctctgtaaag tttgaattaa gtttttctag
    26881 gatttcttga tattcaccaa gtagctttaa ttcttcagaa gttccattaa atacttgttc
    26941 aagtacagtt gaattttcat tgtaagttgg attttgaggt agatattcta tcctaatacc
    27001 attagttttt gttatattac cagtctcact ttcttcaact cctgcaataa ttttgagtaa
    27061 ggtagattta ccagtaccat ttacaccaat aagtcctatt ttttcccctt catttattcc
    27121 aagagatata ttttctagta attttttttc agaatagctt ttacttatat tttccaatgt
    27181 cattaaattc atataattca cctttatttc taatctatta tttttcaaaa tgttgataat
    27241 ctgggttttt ccaatagcca ccccaactcc aacctctgct tacaaaagca ttataacaat
    27301 catcaccttt ttttatcatt ccaaattttg tgctagacct atcagcataa actgtactta
    27361 ctttaggtgt aacatcattt ccagaaactt gaggattctg taatgggttt atatctatag
    27421 cacgtccttt accatgattt gaaactacat ttgtaccagc tatagttcta taacaaaatg
    27481 atgaggtatt attatcagac atagattttt catctacagc atcatagtca tctattaact
    27541 taattttttc aattggatat tttttttcgt ataattcctt aaaaatatcc actacttctg
    27601 gagctagttc actgtttact atcatttctc cttgatgagt tttttcatca aatccataat
    27661 atgtaagttt taaataagat agactatcaa agcttatagg ttcatcagta ggcatagatt
    27721 taccaatcat tttatttttt atattatctg gtattgaaga atatgaaaaa gtaggcttga
    27781 tattattatc ttcctgcttt ttggtagagc tttgattatt tttagaatca tctgttgtat
    27841 tatttttatt ttgcatagtt tcttgactct ttatattacc ttttttgttg ggattagaga
    27901 acagtatctc atatatagat aataaagcaa gaaataatac agttattaca atcgttaatt
    27961 ttaatttatt aatttttctt cgttttgact ttttactcat attatccccc agtattagat
    28021 tcatataaac tatatatatt atacctcata aaaataaact aggctaacaa tatttagtta
    28081 acctagaaca tttcatttat agtttctcca attattttta ttccaatttc aatgtcttca
    28141 aaagacaacc tagaaaatcc gagtcttaaa gtatcaaaac cagagttatc tgtaaaaaat
    28201 aaatctccag gcataaatat aacatttttt tcataacatt tttctaataa aactctagta
    28261 tctatatttt ttagttttat aaatatatgc aaaccaccat cacccaagat atattctgtt
    28321 tttatatatt tttttataca cttatatgca aagttaaatt tgtctccata gaatttacgt
    28381 atttttttaa tgtatttttc aaaacctcca ttacttaaat aatcaaataa aattccttgg
    28441 tctaaaaagg atacatgtat gtttctgcac ctttttacac tttctagttt attgattact
    28501 tttttatctg caaatatcca tccaattctc attccaggaa ataaaatctt tgaaaaacta
    28561 ccaatatata cgaccccatt attcatatta tctagggaac agataggaaa tatatgagaa
    28621 ctattgtaga gtaattcttc attaaatcca tcttctataa ttgctatatt gtattttttc
    28681 ataagattat agaatttgta cctattttca ggagtcatta caattcctgt aggattgtga
    28741 taagagggtg taatgtatgc aaactttatt ttatttttat acttaactaa attgtcttct
    28801 agcatattga agtccaaacc atcttcattt atatctacac ctaagatatt gaggttatga
    28861 gatttcataa tttttatagc agtattatga gtaggatttt cacatattat gtagtctttt
    28921 tcttgggtaa aagatgacat aataatatca aagccttctg tgaatccatt tgttattagt
    28981 atatctttat tacttatatc agcacccttg tttgtcatat attctagtaa atagtcaata
    29041 agtggcttat aaccttgtgc atatccatag ttgagtaatt tatgtccttc tagtgaaatt
    29101 ctatttaaaa aggatttttt taattcttcc atgtcgaata aatctccatc tggagaaata
    29161 cttttaaagg aaatcaggtc agagctccaa ggaatttcac tttttataat gtccaattca
    29221 ttggcttttt tagagtatgt attctctaaa caatcccagt ttaatgacca tgtagtattt
    29281 gaagacttat tctttgattt aacaaatgta ccttttcctg aaatagagta gattaatcct
    29341 tctgatttta attcttcata agttaaaact acagaatttc ggctaacttg taaaagctga
    29401 ctaagctccc ttgtagaagg gagcttacta ttgtctggaa ttagtccttt tgatatcata
    29461 tcacttatat aatttttaag ttgtatataa attggttcat tatcatttaa ttttagattt
    29521 gaaaatatca aattaccacc tcaaattatt tcatctaatt taaagagtat tctataagtt
    29581 tatctaatag ttcactgtac ttaatacctc tagcagcagc acttctagga attaaactag
    29641 tttgagtcat tccaggaaga gtatttagtt caagaacata tggaatacct tcacttataa
    29701 tcatatcaac tcttacatat gctttgcaat taaaaatttt ccaacaagtc tcagaaattt
    29761 cgtttactct tttttgaagt tcttcatcta aataaacaac ttcttccttt gcaccttttt
    29821 caactgagta tttagcttca taatcaaaga atcctgaatc agattttata gatattgtag
    29881 ggaacacttc tccatttaat atgaatgaag tatattctcc acctggtata tatttttcaa
    29941 tcattacaaa ttcatcaact tccagacctt ttctaactgc ttcttcaact tcatccttag
    30001 aatgaataaa gaatgtggct acacttgaac ctcctgagtt tggttttata aatactggat
    30061 aacctatatt atctatttca tcatagtcta tttcatcaac tgattttact aaaacccatg
    30121 gagcagtagg taggtttgaa tctctaagca tttttttagt tatattttta tccatacaca
    30181 tcccactgca aagaggtcca catccagagt atggtatatc catagtctca agtattgatt
    30241 gtatacatcc atcttcacca aattttccat gtaaagcaag tattgcaaaa tctatatctt
    30301 ctggtatctt tgtaaatata tcttttttat catctattat tacttttaca acttcatatt
    30361 tatttttatc aaggttatta tatatctctt ttcctgaatt taaagaaact tctctctctg
    30421 atgatattcc acccattata actgctattt tcatattata cactcctatt ttaattttta
    30481 ttattttaaa gtaaactttt tatttacata cttaattata tgaattaaca gttgaggatg
    30541 aaagtaccac tatatacgaa gtttatatta tgtgtggttt tagaataata atctagttta
    30601 aagatatgtt taaatgattt tttaacttta tttatgttat tatttttgct aaaattattg
    30661 ttatgaactt aagactagtc ttaatggcta taaatcaagg agtgataaat tttgaaagct
    30721 gaaataataa gtgtaggaac agaaatatta ttgggagata tagtaaatac aaattctcaa
    30781 tttttagcta aagaacttgc atcattggga atagaagtat accatcagag tactgttgga
    30841 gataacaaac aaagactttt agaatgtttt gatgaaagct taaagagaag tgattttgta
    30901 attactacag gtggtcttgg gccaactggt gatgatatga caaaagaaac tgctgcagaa
    30961 tactttggtc agaaattaga actacataaa ccttcactag aggttttgga atctttcttt
    31021 gttaaaacag ggaaaaaaat ggccgaaaat aatatgaaac aagtgtactt tccaaaagat
    31081 gctatagtac taaaaaataa taatggtaca gctccagggg caattttaaa aaaagatggt
    31141 aaatttataa tagtattgcc tggacctcca agagaaatga aagctatgtt taatgagagt
    31201 gtcaaacctt acttacaaca attcacaaat gaaatgctag tatctaagac tttaagatta
    31261 tatggaatag gtgagtcaaa tctagaatta gaaattctcg atataattaa tgagcagact
    31321 aatccaacag ttgctttata tgcaaaagaa ttagaagtta caataagaat aactgcaaag
    31381 gctgaaaatg aaagagaagc ttttaaatta ataaaaccag tagaagaaaa aataaaaagt
    31441 agagttggta aatatgtata tactgaagga gatatttcca tatctgaagg ggagactgct
    31501 ttagaagatg ctgtttctaa gctacttgta gaaaaaaatc taacaatagc agtggctgaa
    31561 tcttgtactg gaggtttggt ttcttcatct cttataaact atccaggtat atcctctgta
    31621 tttcttgaag gttgtgtaac atattctaat gattctaaaa tgaaaagatt aggagtaaaa
    31681 agagaaactt tggaagagtt tggagcagta agtgagcaaa cagctataga aatggctgag
    31741 ggcgttgcta aaggacttaa agctaatata ggtatatcta caactggagt agctggacct
    31801 ggtggaggaa ccaaagagaa gcctgtggga ctggtttata ctgcaatata tataaatgga
    31861 aaaacaatag taaagaaaaa tatatttaat ggggatagaa gaaagataag attaagagct
    31921 actagagatt tgcttaatga attgagaata caattagaaa agctttaagt tattttgatt
    31981 aaagtattag taaaatataa aattgttttt ggaggctttt aatttatgat aaaacatata
    32041 ttttgtgatt tagatggaac tctatatgaa aatggaacca taacaaaaga agatatagta
    32101 gcaattgaag aaatagaaaa aaagggagta cagtttaatg ttgctacagg tagaattttt
    32161 aaacaagcac ataatattat caaagatagt ttagatatga atggatatta tgtatgtgaa
    32221 aatggttcat ttatatatga taaagattat aatgtaattt ttaaaagaac gatagatgat
    32281 aatttagtaa aaaaagtcat tgatagattt gagtcaagtg atgcacaact ttattttaaa
    32341 tataagggtg atgtaattgt gggtagtgat accactgctt ttagacatta ttctagtgac
    32401 tttatagttg acccagattt tgaaaaaaga agtagttttg ataatcttat aggaaatata
    32461 ggcgtatgtt ctgaaaattt ggaagagtta tccagaatag aactttatct aaaaagtgag
    32521 tttagtgaag tcttagaaat atatttttct ggaacatata ctttaaatat agttcctaaa
    32581 agtgtgtcaa aaagaggaag tatagagcat gtaataaaaa cgttaaatgt aagtccagat
    32641 gaagttgcta ctattgggga ttctccaaat gatatttgca tgttagaagg atttaaatat
    32701 agttttgcaa tgtctaaggc aagggaagat gtaaagcaaa gtgcaaatta tgtagtagat
    32761 agtgtaaaaa gtgccataga tgttataatg gagataaata gttagtataa aaaatgactg
    32821 tcctagagaa gtataaaatt atattctata gagcagtcat tttaattata ttcaattata
    32881 agtttatata agtttaaaaa ttcttaagtt aaccgcaatt tgctatatga tatgatttta
    32941 ttgtattaga taaaatagag tcaagtatat aatttatgac actattacaa gtataagctg
    33001 aaaattttaa atcattcata taatcagttt tctttttgta atcactataa agtttagtaa
    33061 aaaacttctc aaaactacta tttatagtac cacatttaat tttagataaa tcaacatctt
    33121 ttgcaaatgc tccaagttct ctttcataag taacatgttt atttaagcta tgcttaaact
    33181 cccaacgttc actgtgagct acacagaaaa aatcacataa aaaatgacat atgactccta
    33241 gttcctgact aagtctactt actgaaaagt atttagaaag actatctata ttaagactgc
    33301 atagatagtt tattttagaa acaatcatat taaatgactc atctaaataa tgttttttta
    33361 atttatattt agaaaaagca tcaggcttta tattgccata tataaaattt ttttcgctta
    33421 taaagaatgt tttattttca tctatattat caagtattga ttttgccata ataaaatggg
    33481 tattcat
//
LOCUS       DAEMIK010000028        33060 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEMIK010000028 DAEMIK010000000
VERSION     DAEMIK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.028"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            85..1254
                     /locus_tag="KPK87_001127"
     CDS             85..1254
                     /locus_tag="KPK87_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2537296.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            1303..2154
                     /locus_tag="KPK87_001128"
     CDS             1303..2154
                     /locus_tag="KPK87_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG2537297.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            2480..4381
                     /locus_tag="KPK87_001129"
     CDS             2480..4381
                     /locus_tag="KPK87_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2537298.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            4384..5427
                     /gene="ypdE"
                     /locus_tag="KPK87_001130"
     CDS             4384..5427
                     /gene="ypdE"
                     /locus_tag="KPK87_001130"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2537299.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            5442..5747
                     /locus_tag="KPK87_001131"
     CDS             5442..5747
                     /locus_tag="KPK87_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2537300.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            5844..6179
                     /locus_tag="KPK87_001132"
     CDS             5844..6179
                     /locus_tag="KPK87_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2537301.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            6211..7533
                     /locus_tag="KPK87_001133"
     CDS             6211..7533
                     /locus_tag="KPK87_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2537302.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETITHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            7543..8613
                     /locus_tag="KPK87_001134"
     CDS             7543..8613
                     /locus_tag="KPK87_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2537303.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGKYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            8618..9805
                     /locus_tag="KPK87_001135"
     CDS             8618..9805
                     /locus_tag="KPK87_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2537304.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKDFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(10002..10874)
                     /locus_tag="KPK87_001136"
     CDS             complement(10002..10874)
                     /locus_tag="KPK87_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG2537305.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(11268..11459)
                     /locus_tag="KPK87_001137"
     CDS             complement(11268..11459)
                     /locus_tag="KPK87_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537306.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            11679..12725
                     /gene="cobT"
                     /locus_tag="KPK87_001138"
     CDS             11679..12725
                     /gene="cobT"
                     /locus_tag="KPK87_001138"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG2537307.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            12757..13317
                     /gene="cobU"
                     /locus_tag="KPK87_001139"
     CDS             12757..13317
                     /gene="cobU"
                     /locus_tag="KPK87_001139"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG2537308.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            13327..14100
                     /gene="cobS"
                     /locus_tag="KPK87_001140"
     CDS             13327..14100
                     /gene="cobS"
                     /locus_tag="KPK87_001140"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG2537309.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            14120..14734
                     /locus_tag="KPK87_001141"
     CDS             14120..14734
                     /locus_tag="KPK87_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG2537310.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      14801..14980
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            15109..16608
                     /locus_tag="KPK87_001142"
     CDS             15109..16608
                     /locus_tag="KPK87_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG2537311.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            16647..18023
                     /locus_tag="KPK87_001143"
     CDS             16647..18023
                     /locus_tag="KPK87_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG2537312.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            18024..18995
                     /locus_tag="KPK87_001144"
     CDS             18024..18995
                     /locus_tag="KPK87_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG2537313.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYVIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            19027..20097
                     /locus_tag="KPK87_001145"
     CDS             19027..20097
                     /locus_tag="KPK87_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2537314.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            20099..20986
                     /locus_tag="KPK87_001146"
     CDS             20099..20986
                     /locus_tag="KPK87_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG2537315.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNHLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            20974..21606
                     /locus_tag="KPK87_001147"
     CDS             20974..21606
                     /locus_tag="KPK87_001147"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG2537316.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            21615..22841
                     /locus_tag="KPK87_001148"
     CDS             21615..22841
                     /locus_tag="KPK87_001148"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG2537317.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNIDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            22838..23446
                     /locus_tag="KPK87_001149"
     CDS             22838..23446
                     /locus_tag="KPK87_001149"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG2537318.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            23436..24011
                     /locus_tag="KPK87_001150"
     CDS             23436..24011
                     /locus_tag="KPK87_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG2537319.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            24004..24756
                     /locus_tag="KPK87_001151"
     CDS             24004..24756
                     /locus_tag="KPK87_001151"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG2537320.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            24740..25861
                     /gene="cbiG"
                     /locus_tag="KPK87_001152"
     CDS             24740..25861
                     /gene="cbiG"
                     /locus_tag="KPK87_001152"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG2537321.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            25858..26583
                     /gene="cobJ"
                     /locus_tag="KPK87_001153"
     CDS             25858..26583
                     /gene="cobJ"
                     /locus_tag="KPK87_001153"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG2537322.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            26580..27332
                     /gene="cobK"
                     /locus_tag="KPK87_001154"
     CDS             26580..27332
                     /gene="cobK"
                     /locus_tag="KPK87_001154"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG2537323.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEINYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            27351..28829
                     /locus_tag="KPK87_001155"
     CDS             27351..28829
                     /locus_tag="KPK87_001155"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG2537324.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIGDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            28859..29764
                     /gene="hemC"
                     /locus_tag="KPK87_001156"
     CDS             28859..29764
                     /gene="hemC"
                     /locus_tag="KPK87_001156"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG2537325.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            29754..31253
                     /gene="cobA"
                     /locus_tag="KPK87_001157"
     CDS             29754..31253
                     /gene="cobA"
                     /locus_tag="KPK87_001157"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG2537326.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            31270..32235
                     /gene="hemB"
                     /locus_tag="KPK87_001158"
     CDS             31270..32235
                     /gene="hemB"
                     /locus_tag="KPK87_001158"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG2537327.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            32388..32966
                     /locus_tag="KPK87_001159"
     CDS             32388..32966
                     /locus_tag="KPK87_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG2537328.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        13380 a         3312 c         6104 g        10264 t
ORIGIN      
        1 aattataaaa gagtatattt ttttatagtt taaagtggtt ataacctcat taccaaatgt
       61 aaacgttaca agtatgaaag gagaatgaaa atgtttaaat tagaagaaca aaaactaaaa
      121 gatttaggtg caataataac aactaatgaa ataaaacaac agcctgaatt atggttagaa
      181 acttatgaaa tatataaatc taacaaagaa aaattaagta gattcataga tacaataagt
      241 aataatcatg ggcagtttag agttatattt acaggtgctg gaacttcagc ttacatagga
      301 aattcaatac ttccttacct taaaaataaa aatgacataa gaaaatacat atttgaagca
      361 atacctacta cggatatagt ttcaaaccca tatgactact taaaaaaaga tataccaaca
      421 ttattaattt catttgcaag aagtggaaat agtcctgaaa gtttggctgc tttaaattta
      481 ggaaataaga ttgtagataa tttttatcat ttagctataa cttgtaatcc agaaggagaa
      541 ctggctaaga tgactaaaaa cgacgaaaat aattacttgt tattaatgcc atcaaaatca
      601 aatgatgaag gctttgccat gacaggaagt ttttcgtgta tgatgttatc tgctatgtta
      661 atattcgata gtttagaaga tgatgtagaa aagtcatata taaatgcaat aattgaaatg
      721 ggaagaaatg ttatagatag aaaagatgaa atacatgagt taataaataa agattttgat
      781 agagttgtat atttaggttc aggaggatta ggtggtttga cacaagaagc acaactaaaa
      841 cttttagaac ttacagcagg aaaaatatct acagtgtatg attctcctat ggggtttaga
      901 cacggaccaa aatcatttat tgatgaaaat acacttgtat ttgaatttgt atctaactgt
      961 ttatatacaa gaaaatatga tttagatgtt ttagaggaga taaaaaggga taaaattgct
     1021 aaattcactt gtgcagtgtc agtagaaaat gaaaataact tctcaggaac aaagtttgag
     1081 tttaaagaaa agtataataa attaccagat gtgtatcttg caatgccata catattgttt
     1141 gcacaaacta tagcattgtt tgtatcggta aaggttggaa ataagccaga tactccatca
     1201 gctacaggaa cagtaaatcg agtagtaaaa ggtgtaacaa tatatgaata ttaaaagata
     1261 agattaaaat attataacta aataatttgg gagggtatca atgtggcatt agtaacaaca
     1321 acatcaatgc ttaaaaaggc acaagatgaa gggtatgcag taggagcatt taatgtagaa
     1381 aatatggaaa tggtaattgc tgttatagaa gctgctgagg agttaaattc tccagtaata
     1441 ttacaaacta ctccatctac tgtaaaatat gcaggtcttg attattatct agcaaatgca
     1501 aaggttgctg cagaaagagt aaatatacct gtagcaatgc atctggacca tggttcaagt
     1561 tttgaccttg caatgaaagc tttaagaagt ggatatacat caataatgat agatggttct
     1621 catgaaagct ttgaaaataa tatatctata tctaaatcag tagtaaaagc atgtagtcct
     1681 tctcttatac cagtggaagc agaacttggg aaagttggtg gaaaagaaga tgatttagat
     1741 ggcgggaata gtaatgatta tacaaatcca aaagaggcaa aagagtttgt cgatagaaca
     1801 ggaatatctt cattggctat tgctataggt actgcacatg gattgtataa gggtgaacct
     1861 aaaatagatt tagataggtt atcagaaatt aaaaatgtag tttcaatacc tttagtattg
     1921 catggaggtt caggaataca tgatgatatt ataaaagaag ctataaaaag aggtattgca
     1981 aaagttaact atgcaactga attaagaatt gcatatagta aaggggttaa aaaagtttta
     2041 gatgaagatt ctgaggttat agaccctaaa aagtatggat tagcagggct tcaatcagta
     2101 aaggattttg tgaaagaaaa gattaaagtg tgtgggtctg taaatagagt ataaaaataa
     2161 aatatcctaa atttatttta gataagttta ggatatttgt tatatgaaaa atttatatta
     2221 attatatgta aatggtgtat ttttttcatt taattgatga aaaatatcag cttttattgg
     2281 taaagtatta catatataat taaaatatac taaaaattga actatatatt ttattattat
     2341 aaaatattta tattaaattg tggaattaaa atatactagg atataaaatt cttaaataaa
     2401 aagaaaaggt tgtttttata aaaaatattt tttataccac tttaaaaaac acaataataa
     2461 cactgaggga ggtggggata tgagagaacg cattattctt gaaacattaa aaaatagtag
     2521 tggttatcta aacatagatt attttgcaga taagttgggg gtatctactc gaactatcag
     2581 aaatgaaata aaaaagatag aaactataaa agaatgtaat ggttttaaat tagaatataa
     2641 gtctaaatta ggatatattc taaatattaa agaccaagat aaattcgagc attatctaag
     2701 aaacttaccc tcagatttag ttgaaaatcc agaacaacgg ttggagtcta taatagtaga
     2761 gcttttagta aatgaaggtt ataaaactat agaacagtta agtaaaaaat ttctagtaag
     2821 ttcatcccaa ataaaaaatg atttaaaaaa gatagatgaa aagataaagg atacagaatt
     2881 aaaactagag cgaaaggctc attatggaat taaaatagaa ggttctataa aagaaattca
     2941 aagtatattg gtggatagtt attttagagg gaatagaaat ataacagagt acaggaataa
     3001 gtttatagat aatttaaaat tagctaatat tagaagtaca ataaaaaatg tacttaatga
     3061 acatgattta gaagcaaatt taacagaact tgaggaaata ttagcacaaa ttattatatt
     3121 gtatataaga gttagtatga gagtagcggg aaatgctagc gatttaaaat tatatacaga
     3181 agatttaatt atagataaat tattagataa aatttttaga gataaaaaat ataaccttaa
     3241 ttatgatgaa aagtattatt taaaggagct tataaaacta aagactaaag ataagaaagc
     3301 cactataaag aatattgata agaataaact tcaagatatt atgttcgaat tttttagaga
     3361 gatagataaa aaatataact ctaatttttt agaagataaa gagttcttta acttatttta
     3421 cttacatatt gcatgtttaa tagaacgtat aaagaagaac cacaagataa taaatccttt
     3481 ttcagttaaa ataagtcaac agtatccaac tgtttttaat ttatctattc aattttcaaa
     3541 aatcatagaa aatgagtatc atataaaaat aagtcaagat gaaatagggt ttatagctac
     3601 tcacattgct gttccttttg aaaagagaga agaagctaat tttaataaaa aatataaaat
     3661 agcaataata tgctcttctg gtggaggtag tgcatttttg ataaatttaa gacttaaaga
     3721 aatttttcca aatgctgaga taagaaattt ttcattacta gaagaaaaag ctgtaataga
     3781 atttgcacca gatttgattt tttcaataac aaatctttta tttgaaataa atgctccagt
     3841 tatacttata aatgaaattt tagacgaatt ggattattta gatattaaag aaagtgttag
     3901 atttgcagac catataggaa atatatcaag cccaaaacaa tatatattag gtctatttga
     3961 taagaatcac tttaggtgta taaaagataa agctaagtat aaggatatcc ttgatagtat
     4021 gtcaaaatgt atagttgacg agggagcttg ttctccaacc tatcccaaag atgtatggga
     4081 aagagaaagc tatttgagta ctatttatac aaatggggtg gctatacctc atccaatcga
     4141 gatgacagga aacaaaaata taatatcagt agctttgata caaactgata ttatttatga
     4201 aaatagggtg cccaaaatta ttttcatgat ttcattaata aagggaaatt tagaacttca
     4261 taagcagata tctaaatatt taagtaagat tatggcaaat aaggatatgg tagacatgct
     4321 aaataagtca caatcttatg aagagtttat gtataagtta aaaatctatc tgggaggtta
     4381 gacatgaata tagagttttt taagaggttg tcagaagcag atggtatagc atctaatgaa
     4441 gaagaggtta gacatgtttt attggaagaa ctaaaagctt atagtgataa aattatctgt
     4501 gatgggctag ggagtatcat cttttctaaa ataaaagatg agagtgcacc aaacgtaatg
     4561 atttgtgctc atatggatga agttggattt atggtgagaa gtatagacaa acttggtatg
     4621 attcacttaa taacaatagg aggagtaaaa cctcttgcac agtttgtaca aaaagttaga
     4681 attacaacaa aagaagggaa gaaaatcccc gctgtcataa atgctacata taataatggt
     4741 aaggctgaaa atatatatgc agatattgga gcatatacag aagaagatgt atataaccta
     4801 gggattaatg ttggggatat ggtaacatat acaacaagct ttgaggaatt tactttacca
     4861 gaccgattag tgggaaaggc ttttgatgat aggataggat gttttgtgat gggagaagtt
     4921 ttaaaagaac ttaggaaaga aaatctaaat tgtaatattc actttgcggc tacaagtagt
     4981 gaagaggttg gaataagggg agcaaagaca tcaacacagt taataaatcc agatatagta
     5041 tttgtaatag atgtagcatg tgctaaaaat gagtttgtga gagactatac aaatcagaaa
     5101 caaattgata aaggtgtaat gctaatgcat agagatagga ctctagtccc tagtagaaaa
     5161 atgatagatt atgtaatgga aattgctgat aaaaataata taccacttca acatgatatg
     5221 tttgaatcag gtgggactga tggaggagaa gcacatctcg taaatgaagg gaaaccttgt
     5281 gtagtaactt gtgtaccagt tagatatggt cattgtgcat tttcaatagt aagtaataaa
     5341 gatttggaga acattataag attgtataca cagttaattt taaattttga tgaagaacaa
     5401 tataaacata taagaaattt tatctaaagg agaataagaa aatgtcaaca gaagtacaaa
     5461 taatggaact tatatcaact gcaggagaga gtaaatcgaa agcttttgag gctttaaaaa
     5521 aagtgaagtc aaaagatttt aaaggagcaa aattgttaat agaggaggct cgtaaaatag
     5581 atattgaagc tcacaggata caaacagaat taataactca agatttaaat gaagatgttg
     5641 aaagtccagc aatgagtctt ttaatggtac atgctcaaga ccactatatg acttctcagc
     5701 tagcaaaaga cttgatagag gctttgatat attgtctcga taagtaattg taatgtagaa
     5761 atagattatt tatgtaaaaa ttcttttaaa agtttagatg cttttaagag ataataaaaa
     5821 tacaaacttt aggaggaatt attatgaaaa tattattatg ttgtgcaggt ggattttcta
     5881 ctaatatgtt aatgcaaaat atgaaaaagg tggtaaaaga gagtgctaaa ctcaatgaaa
     5941 aagattttga gtttacagct ataccagcag attcaataga aagtgaagtt gatgattggg
     6001 atatagtact tataggtcct caaatagctc acaaaataga cttcttaaaa ggaatattag
     6061 agccaaaaaa taagcctttt gcagtaatag ataaagatgt atatggaaaa atggatggag
     6121 ctacagttat gaagttggca ttaataactt ataagaaaca tcaattagct caaggataat
     6181 aaaaataatg agggaaaagg gagattagat atgttttcaa aatttgaagc atttatgaat
     6241 agagtattta tgcctttagc tcataaggtt gacaatcaac gtcaccttgg ttctataaag
     6301 gctggtatgg ttgctatgac accattcaca atattaggaa gtatatttgc aatacttcca
     6361 gcacttccaa atatgcttgg agaaaataat ccaataagca aattcatact taacaattca
     6421 gcaatattgg atttaccagt aagtctttcc ataggtttaa taggattata tgcttgtatg
     6481 tgtattgctt ttaatctggg gaactactat aaattatata taccaggttg tgttgcacta
     6541 tcaacttttt cctttttatt cctagtagcc tcatttacac cagatggagc tttagaaata
     6601 ggaaatttag gagctaaagg gctatttaca ggtatgatag cagggcttgg ttctgtggag
     6661 ttatataatt tttgtaataa gaagaatatc acaataaaaa tgccagaaaa tgtacctgat
     6721 tttgtatcta ggtcttttga attaatacct actactataa tagtttgtgg aacttttata
     6781 gttgctcgtt ttatttcatt aaaagtattt ggagaattac ctccacagat acttactcaa
     6841 tttttatctc cattagtagg aagtatggat aacccatggt cagttcttgc acttaacttt
     6901 gctatatgta ctatattctt ttttggaata cattcatcag tatttagtcc tataactaga
     6961 cctataatga ttacatttat agctgaaaac atagcagcta tgcaggctgg agaaacaata
     7021 actcatttct ttacagcagg agcttcaagt gcattctttg gatttacagg atgtggaatt
     7081 agtataggtt gtgtaattgc atgtatgtta tcaaaaaatg cttcctataa gaagattgga
     7141 cgtgtatcat tattccctgc attatttggg ataaatgaac caatattatt tggagcaccg
     7201 ataatattaa atccaataat gtttatacca catgtatttg gtggagctat aataggtaca
     7261 ctaccaatgt tttttatgca ctggggatgg ttagcaaagc cagtatttga ccctccatat
     7321 gtaggggtat tcttagaagg atttttaact aatggagatt ggcattctat attagcaaat
     7381 gcattgcaat tagtattagc agttttatta tattggccat tctttaaaat aatggaacgt
     7441 caacaaaata ataaaaaaca aaaagaagaa actaagaata tattctctaa agaagaagaa
     7501 gacttattgg atgatttagg attggatttc taatattttg ttatgaataa tacaatggag
     7561 agattagtat ccttaatcta taaaagaggt gttgacgctc ttttgattaa agcaaaaaat
     7621 actaaaagat atataggagc tttatcaggt agtggtgtat atgtgcttgt tactaaggat
     7681 aaaaattatc aaattctaga tggcagatat attgatgaag cagataaaaa aacgactggt
     7741 ttcataaaaa ggattgtatc tcaaggttct tatataccaa ctataattga gttattaaaa
     7801 gaattaaata taaaaaagtt agcaatagaa aaccaagcta tgagtataca agagtatctt
     7861 tcactaaaaa atgaaggttt tgaaatcaat ttaataacaa atgaattatg gaagctaagg
     7921 gctataaaat ctaaagaaga aattgaactt attaaaaagg cttgtgaaat tacagatgaa
     7981 gtatttgatg aggttatttc tgaaattaaa aaagatatga ctgagctaga agtatcagct
     8041 ctaattcagt atcatgcatt aaaaaaaggt gcaagtggaa tgtcttttga aacaatagtg
     8101 gtatcaggag aaagaggtgc aatgccacat ggaagaccta caaacaaaaa attgaaagtg
     8161 aatgaagcta taacaataga ttttggtgtt gtttatcaag ggtatcaatc agacatgact
     8221 cgtacaatat ctataggtaa acctcctaaa attattaaag agatatatga tgtagtattg
     8281 gaagcacaat tatctgcaat agagtcaata aaggaaggaa ctagggcaag tgatgttgac
     8341 aaagtggcta gaaaaattat agataaacat ggatttggca aatattttaa tcatggactg
     8401 ggtcatggta taggtcttgg agatggtgaa gtccccactt taaatccaaa tagtgaagat
     8461 atattagtgg aaggaatggt aatgtcttgt gagccaggta tttatattcc aaatgtaggg
     8521 ggagttagaa tagaagatga cattgttata atagatggaa aaggtgttcc actcaataag
     8581 acatccaaag agtttataat attaggagag taaaattatg aaatataatt ttaataaagt
     8641 attaaatcgt aaagaaggaa attgtagaaa atggagcaac catgtgatta aagaaaaatt
     8701 cggcttaaat gaagatgcaa ttcctatgga tttagctgat atcgattttg agtgtgctcc
     8761 tgctataaaa gaatctatct taaaaagagc atcagtagga gattatagtt atacttttat
     8821 aggagatgac ttctatgatg cgattataaa ttggaataaa agaagatttg atgtatatat
     8881 agaaaaggat tggataaaat taacttttgg tactgtttca acccttcatt atatagtcca
     8941 agcatatact aaagaaggag agggtgtatt gataaataca cctgcttatg acccttttgc
     9001 agaggctgta gtaaataata atagaaagtt gtgttgtagc tcattaaaat tagataacaa
     9061 tagatattat ttagatttag aggatatgga aaatcaaatg aaatacgaga atataagatt
     9121 gcttatattc tgtagtccac aaaatccatc aggtcgtata tggacaaaag aagaactata
     9181 tcaagtgtca gagttatgtt taaagtataa tgtaattttg gtaagtgatg aaattcatag
     9241 agatataata tttaaagatt atagatttgt atctttatgg aatgctcatc cggaaattta
     9301 taaaaatagt ataatctgtg tatctcctaa taaaggcttc aacttaggtg gattaaaaac
     9361 atcatatgta ttaataagag atgtaaaaat tagggagact ttattagaaa ggctaaaaag
     9421 taattcaata acttctccaa atgtatttgc aataccagca atcgttgcag cgtacaatga
     9481 aagcgaagaa tggctagatg ctatgactag ttatgtagag gagaattttg aaatagttta
     9541 tgatttcttt gaaactaata taccaaaagc caaagtgatg aagtcagact cttctttttt
     9601 agcctggata gatgtaagag aagtgtttag aaatgaagaa gaatctaaag acttttttag
     9661 acatgcaaac ttaactatgg tagttggaag ttattttgtg aaagatggag atggatttat
     9721 tcgtattaat gtggggtgtc ctagagaaac attaaatgag gctctaaata gaatcaaaaa
     9781 aacgtatata agtatgtatt gttaaatcat aaaaaaagta tctatggttt atataaaaac
     9841 acattattta gttatcaaat attactggaa ttttgtattc cagtaatatt ttttgattaa
     9901 ggaatattat tgaggaatat taaataatat tatttcatac ccttatatta acttatataa
     9961 aaatatttta ttattttgga aacgggttag ctaaccttta tctagtgtat atttatattg
    10021 taagaagcag taaaaacttt tcctttacta agtttattga taaacttctt atctttataa
    10081 attttgttag aatttatact atcagcaaga ccataccaag gttctataca taaaaaagga
    10141 gcagtagaat tagtttcact ataataagga gtccaaatac caactagagg gaaatcttcc
    10201 atagatatag ttacatattt actaccatca ttattacata tagatacttc atctatgttg
    10261 gtgtatatca aagcatcatt cttgaatact tctggattta actgtaagtt ttttaatttt
    10321 ccaataggta ttatatcatc tgtaaaagaa ccatttaaat tgattttttc tacatcattt
    10381 ttacttttaa actcaaggta gtgtttagaa aaatcatttt gttcgtaaaa aggaaggttg
    10441 aatgcaggat gtccacctat agaaaagaat atatctttag aatcagtatt ttttacaatc
    10501 cattctacct caatactatt atcatttaat gtatagttta tgattagctc aaaagaatat
    10561 ggatacttct ttaaagtaga ttcattatct gttaatttat aagttacaaa tgtatctcct
    10621 ttatctacaa tctcaaagtc catatctcta gcaaacccat gttgagtcat gctatataaa
    10681 ttttcttcta ttattgtttc attatcaaaa agtttaccta ctatagggaa caatataggt
    10741 gattgtctgc cccaatattt tttattacca tcccaaagaa tgtttttgtc gtatttttta
    10801 gaaaatattt tagttagttc agcaccaaaa ctgcttgatt cgataattaa attttcattt
    10861 tgtaatatat tcatttaaaa tctcctttat tatattttat gagtatatta tactataaag
    10921 tataaagatt attaaatttt atttgatatt atatttgaaa ggtagatttt agtatatttt
    10981 gaaaattttg ttttgaaaat ttgggattat ttaagaaatt ttgtattata aatagaagga
    11041 aatagataaa ttgtatagaa taaaatatat taagataagt taataatgtt aaatgggaag
    11101 tgagtttatg gaaaaaccaa atacaaaagt tgttaacttt caagagtata aagacagaaa
    11161 aaaccaaaga gaagtaaata gagacgaact tttaaagctt ataaaaaaaa ttgtgacttc
    11221 aaaataggga gttgttaaac tccctatttt aatgggtttc ataataatta agacattctg
    11281 agtccatcta tataatcttt gaattgtgag aaatctgatt cactacttaa ttctatggtt
    11341 tctgaacctt ccgtataaaa attcttaaga tagatagtat aattattatc aacacaatca
    11401 tgataaatac atctatatcc atcttcatac aaacttttta acttagaaaa atcatccaca
    11461 atatcatctc cttaaagttc ttttaaatat atttttacaa aaagcatcta attgtatgca
    11521 aattgacaat gtaaaataaa ggaaatgaac ataaaatgta gaataatttt tatatgtatg
    11581 taaaaataaa aagaaaaagt tgaaattttt agaaaataag agtatttttt atctaaaaag
    11641 agagtttaaa aaataaaaaa atgttgggag gataaaaaat gagtttatta gaaagtatat
    11701 caaaaaacat atattcccta gataactctt ctattgaaaa aacaaagcag agattggata
    11761 ggcttataca tccaactgga agcttaggaa agatagaaga tatttgcatg caattagcag
    11821 gaatatttgg caatgaaaat tttgacacaa gtaaaaaggt tataattgct tttgcgggag
    11881 accatggtgt atatgaagaa ggcgtagcac ctgaccctca aaatataaca aaattacagt
    11941 ttccaaattt ttcgaaaggt ctatgtggag ttggagtaat aagtaaattt gtaggggcag
    12001 atgttgtagc tgtagatgta ggaataaact gtgatgaaaa actggatggg gtattagact
    12061 acaagataag aaaagggaca tcaaatatgg caaaaggtcc agctatgagt aaacaagaag
    12121 caattagatg tttagagata ggtattgaaa tagcagagca gtgtatagaa aaagattata
    12181 aggttattgg tattggggag atgggtatag caaatactac tccaagtaca gcaataatat
    12241 ctgtaatttc agggtgtgac ccgttagaag ttactggtat aggtgcaggt cttaagaaag
    12301 aaagactaaa gcataaggct gaagtaatta gaaaggctat tgaaattaat aatccaaatc
    12361 caactgatgg agtagatatt ttatctaaag taggaggatt tgaaataggc tcaatggcag
    12421 gtgttatatt aggttgtagt gctaatagga ttccagtagt aattgatggg tttatttctt
    12481 atgcagcagc attaattgca tataagataa atccaaaaac tagagaatat atgatagcat
    12541 ctcatttatc agcagaatca ggaactaaaa gagcattaga catattaaaa ttagaccctc
    12601 tattaaatat ggatatgaga ttaggtgagg gaagtggagc tgctttagca tttgatataa
    12661 tagaagcttc aaactatact tataaaaata tggcaacttt tgatgaaatt gacatgggaa
    12721 gataaatata aaaatttaat atgtaggagt gttttgatga gtaaaattat tctagtaaca
    12781 ggaggagcta ggtcagggaa gagtaacttt gcagaaaaac tatgttttga cagaaataat
    12841 aatacagctt atatagcaac ctctattcca tttgatgatg aaatgaaaga tagagtaaaa
    12901 aaacatcaag agaatagacc tagaaactgg aatacttatg aaatatatga agatatatat
    12961 tccataatta aggagatata taaagaacat gaaactgtta tactagattg tgtaacatta
    13021 ttagtaaata atctaatgtt tgaacacaat ctaaatatag aagaatctag tcaagaagaa
    13081 ataaacaaat tagaacaata cataaaagaa caagttgtca aacttattga agaaatagaa
    13141 aaaacaaatc tatactttgt atttgtaacg aatgagttag gtatgggaat tgttcctggg
    13201 aataaactta gtagaattta tgctgatatt gtaggtcgta taaatcaata tatagcttcg
    13261 aaaagtaatg aagtttattt tgtagttagt ggcatagcta tgaagataaa ggagtagtat
    13321 gcaattatga aaagatttat tttaatacta cagtttttga caaggatacc tataaaatta
    13381 aatgtaggat ttgatgatga attttataag tcaatagtat attttccact tgtaggtttt
    13441 gtcataggta tattatccta tttaatagga tggatttcta tgcttttatt tgaaccattt
    13501 atagcttcta taataataac tctagctggt gtcttaatta ctggaggact tcatatagat
    13561 ggattaggag atacatttga tgcaatatat agttataggg ataaagaaaa gatgcttgaa
    13621 ataatgaaag attcaagatt aggtacaaat tcacttctag caattatgtt tgtactttta
    13681 ttaaaggttg gattcgttta cgatataata agtaataatt cattgtgggt tataattttt
    13741 atgccaatga ttgcaaggtt aggagtaatg ttattgacat ataaaactgt gacaccaaga
    13801 gaaaaaggaa tgggaaattt atttatagga aaattaacta caagtatgtt aataactgct
    13861 ataatataca cattattgat agttgctctt attactaaat ttatattttt attacctaat
    13921 atagtgttga ttaaagtatt aggttcgatt atagtggttt ttgtatttat aatattattc
    13981 aaaaaacata tttataaaaa aattgacgga gtaacaggag atatattagg ctgtggaata
    14041 gaactttcag agcttgtata tttaatttac atatatttgt taatttttat gtttttttaa
    14101 agtaaaaagt ggtgataata tgataagatt aatattaata agacatgcac tgacaaatga
    14161 taataaaaaa ggaagattat caggccatat aaatagttgt attagtgaag agggcaaact
    14221 acaaataaat aaaattacca gatatctgag taatgaaaac atagataagg tttatacaac
    14281 accatctaca cgtactaaag atacagtaga aaaaatatca aaattgaaat tattagaaat
    14341 agaagaaaaa gaagctttaa gagaaattag ttttggagac tttgaaggaa gaacttttga
    14401 agaaattaaa atcaaatatc caaatgagtt tgaaaaaatg ataaaagaag gtaacaacta
    14461 tagatatcca aatggagaaa gtttgataga ctcttataaa agagtggcga aagaaattga
    14521 taatataatt ttagaaaatg atagtaattt agatacaaag acaatactta tatgttctca
    14581 tgcaggaaca ataagaaata taataacaca tttgatttct ggtagttata agtatcactg
    14641 gaactttaaa attgataatg catctattac agttttagaa attgatggtg gatttgctgt
    14701 aatagataaa atgaatttta cagacttcat ataatttaat caaaaccaaa cattacaatc
    14761 tgtgtttgta atatatctgt aaaattagca atgaaaatag aatattagat taaaattagg
    14821 ttcttaatta gatgttaaaa gggaaaaagg ttagatgcct ttgcagcccc cgctactgtg
    14881 aaaccaacga aactataaat accactgtca gtttgatggg aaggttatag aagtaggatg
    14941 aagttaagcc aggatacctg cctaatttaa tttaaaacaa gaggtcttcg gggtgaaaga
    15001 tttttaatta atatatataa aagtcctatc tttagaagta ttaggacttt ttttatttgt
    15061 tttttagtaa attttaacaa ggagggatgg aaagtgggga aaaaaataat gctacaagga
    15121 acagcttcaa atgtaggtaa aagtgtctta gtagcaggtt tgtgtagaat atttaagcaa
    15181 gatggttata ctgttgctcc ttttaagtca caaaatatgg ctttaaattc ttttataact
    15241 aaagaaggat tagaaatggg aagagcacaa gtatttcaag cagaggctgc gaaaatagag
    15301 ccaatagctg atatgaatcc agttttatta aaaccttctg gaaataataa aagtcaagtt
    15361 attgtaagag gtaaagttgt aggagaaatg ccttcttcta agtatcatga ttataaattg
    15421 gagttagtag atgtgttgaa ggaaacattt aatagcttaa attcaaggta tgatattgta
    15481 gttatggaag gggcaggaag taatgcagag ataaacttaa aagatagaga tatctctaat
    15541 atgggtatgg cagaaatagc tgatgcacct gtaataatag tcggagatat tgatagagga
    15601 ggcgtatttg cctcattagc aggaacaatg cttttattaa atgaagatga gaaaagaaga
    15661 gtaaaaggtg taataatcaa taaattcaga ggaaaaaaag agcttttaca agatggtgta
    15721 aagatgctag aagacatcat aaaagtacct gttataggag tagtacctta tacagatata
    15781 aaaatagaag atgaagacag tgtaactacg agatttaaga aaaaaatgaa taaaagtgat
    15841 atacacatag aaataattag aactccacat atgtcaaatt ttacagattt taatatattt
    15901 gagacacaag aagatgtaag tattagatat gtagactatg gagaatcttt aggaaatcca
    15961 gatatattaa taatacctgg aactaaaagt actatagacg atttaacata tataagaaaa
    16021 agtggtcttg aatcgcaaat caaaaattta catagtcaag gtaaattgat atttgggata
    16081 tgtggtggat accaaatgtt aggtaaaaaa ttaaaagacc cttatcatgt tgaaagtgaa
    16141 attgaagaag ttgatggaat aggtctttta gatacagaaa caacgtttga gaaagaaaag
    16201 actacaacac aagtagaagc tactattgtt gaatgtatca gtgaatacat gaataatctt
    16261 aagggaaaaa aagtaaaagg ttatgaaatt catatgggaa ttacaaagcg aggtaatgat
    16321 gtaagaagtt tagatttaat aactaaaaaa cttgataaag aagtgaatta tactgaggga
    16381 agtgtaaata aagaggggaa tgtaattggg acttatcttc atggaatatt tgatgaaata
    16441 agctttacaa gagcaatttt aaacaatata agaaagaaaa aaggtttagg agaattagaa
    16501 agcaaagtaa actcttttaa tgaatttaag gataaagagt atgataagtt ggctgatttt
    16561 ttaagagaac atttagatat agagaaaata tatgagatta tgaaataatg atgaccttta
    16621 tttataattt aagtaggagg tgtattatga aaaaaatttt aattgcgggt actaatagtg
    16681 gagtaggtaa aacaacaata tcattaggaa taatgcaagc tttggttaag agaaatatga
    16741 aagtacagcc atataaagta gggccagact atatagaccc ttcatatcac acttttataa
    16801 ctggaagaca ttctagaaat ttagattctt atatgttaga tgatgaaaaa ataaagtata
    16861 tagtaaataa gtcctcaaaa gatgcagata tttcagtaat agaaggtgtt atgggcttat
    16921 atgatggctt tggaatagat ttagataatt gtacaagttc acatacttca aaggtattaa
    16981 agtcacctgt tatattagtt ataaatggta aatctatggc agcatctagt gctgctatgg
    17041 tacttggata taaggaacta gataaagatg taaatataaa gggtgttata gtaaataatg
    17101 taaagactaa tagccattat caaataatta agagttctat agaaaaatat tgtaatgttg
    17161 aagtattggg gtattttcct ccaaataaca aattctcttt ggaatctcga catctaggat
    17221 tagttcctag tgtagaaatg aaatcactaa gggaaaaatt ttatacctta gcagatgaaa
    17281 ttgaacacta tatagatatt gacagaataa ttgaaatatc agaaagcgat gaatttgaga
    17341 gtgattttga ttttcaaact ttaatagaaa ataataaaat aaaagaacat gttaataata
    17401 aatcaatagc tatagcatat gataaagcat tcaattttta ttatagagaa aatatagagc
    17461 ttttagagga acttggttta gaaataaact attttagccc tcttgaagat gtatcagtgc
    17521 caaatgcaga ttatatttat attggaggag gttttccaga aatatttgga aaagaattag
    17581 aagcaaatga gtcaatgaga acttccatat tagaagcaca taacaataat atacctattt
    17641 atgcagaatg tggaggactg atgtacctag gtgagaaact attgaatcaa gaagaacaag
    17701 aatttaatat ggttggaata tttagtggaa ttagtaagat gacatcttct ttaaagagat
    17761 ttgggtactg tattggtgta gccaaggaaa atactctttt atctaaaaaa ggggaatcta
    17821 ttaaagggca tgagtttcat cattcaatat ttgaaagtga tgaaaaatgt gtatattcaa
    17881 tgagaaaaga aagagatgga aatatagtag aagagtggga tggtggttat agtaaaggaa
    17941 acactttggc tacatatctt catactcatt tttataataa cttaaattgt atagaaaatt
    18001 ttttgaaaaa aggaaatgaa taaatgagta ttttatctat ttatatagga tatgtgacag
    18061 acttaattgt aggagaccca tattcttttc ctcatccagt gagatttatt ggtaaactta
    18121 taaattttac tcaaggtata ataagaaagg tctctaaaaa tgataggcag ttaaaatttg
    18181 ggggatttat attatggttt ataaccgttg gaataacata tttaacaact tatgttatag
    18241 tgaagttatt tagttttaat atattattga gtattgtagt gaatagtttt ataatttata
    18301 caacattagc cacaaaatgt ttaaaagacg aagcattaaa aatttataat gtattaaaaa
    18361 ctgaagatat agaaaaatct aggattcagc tatcttatat agtaggtagg gatactacta
    18421 atttaagtga acctgaaata ataagagcaa ctgtagaaac tgttgcagaa aatactgtag
    18481 atggtataat tgcgcctatg ttttatgcat ttattggagg agcaccactt gctatggcct
    18541 ataaagctat caatacattg gactcaacag ttggatataa aaataaaaag tataaagata
    18601 ttggttttgc atctgctaag attgatgata tagccaatta tataccagct agaatatctg
    18661 tcattcttat gacaattgga agtttctttt taaattataa ctataggaac tgttttaaaa
    18721 tctctattag agatagaaag aatcataaaa gtccaaattg tgctttttca gaaggtgcag
    18781 tatctggtgc tctaggaata caactaggag gtaccaacgt ttattttggg gaaaaagtat
    18841 ataagccaac aataggggat aagcttaggg agatagataa agaagatata attaaaacta
    18901 ataaaattat gtatgcatct tcatttgtat ctatgttggt atttacacta atatatgtgt
    18961 tatgtaggat gattttaatt aacttaatta tataaattat tgtttgttaa cttaaggggg
    19021 acatagatga aggatttagg gcatggtgcc aatgtagatg aaatggcaag attatatggg
    19081 aaaaacccaa aagaaataat tgattttagt tcaaacataa atccaaatgt attaccaaat
    19141 ttagagagat acattttaaa aggattagaa gagtgtagga attatccaga tataaattat
    19201 acaaacttga gagaaaatat atcaaaatat atagatataa atccagactt tataatacct
    19261 ggcaatggtg caacagaagt gatttatttg cttatgaaga gtattaaaaa aaaattggct
    19321 ataataaatc cgactttttc agagtataga agaagtgctg agttaaataa tttagatatt
    19381 atagatttag agttagattt agaaaataac tttaaactta atatagatat aataaaagaa
    19441 aatataaaaa gatttgatag tttatttata tgtaatccta ataatccgag tggtaatgtc
    19501 caagatttaa aggaacttgt tcatttacta gataaacata ataaggtact aataattgat
    19561 gaaactttca tggaatttgt tgaagatgaa agtaaatata gtttggttaa atatatagaa
    19621 tcaaataaaa atatatttat aattaaagct gtgactaagt tttttggtat gcctggttta
    19681 aggctaggat atggtcttac aagcaacact gaaattatga acaaaattta tgaacacaaa
    19741 gagccatgga ctatcaattc ttttgcagat atactatcca attttatttt tgaagataaa
    19801 gagtatatta aaaatagtaa agaatattac atagaagaaa gaaaatatat gttacaagag
    19861 ttgagaaaca taagaaatat aaaggtctat gatacagatg ctaattttat cttgataaga
    19921 atatataaaa aaacaactaa agaattgaaa aaagatttgt ttaagcaagg aaacatatta
    19981 gttagagatg catctaattt tataggatta gatgatagtt ttataagagt ggctataaaa
    20041 tctcatgaag ataacaaaat tcttatagaa aatatgaaaa atttattggg tgattaaaat
    20101 gaaatcttat ggaatatgcc cagcatcttg tggagaattt gtacaaggta taattgatga
    20161 tgaagaatat ttatgttcat atgcaataga tatgtattct aaagtgtata ttgaagaaaa
    20221 attagtagat ataaatttag ggagatataa gtcaaggtta gcaattgaaa aggtatttga
    20281 aaaatttaat ttacctaaaa aatatactaa aaatatttct ttaaatataa atagtaaaat
    20341 acctgttgga aaaggaatgg caagttcaac tgctgatata ggagctacta taaaggcaac
    20401 attatcattg atagataaag atttaagtag cgaggagatt tctaaattag cagcagaaat
    20461 agaaccaaca gattctatat ttatagataa aaatagtata tttaatcctc taaatggaac
    20521 tgttataaaa tacctaggaa atctgaccaa tgcgaaagtt gtaatacttg aaccaaacaa
    20581 agtactagat actatgaaaa ttagattgag gcaagattat aataaattaa aagtagagaa
    20641 taaagaagta ataaaaaaat catttgctct tttagaagaa ggattgaaaa aaaatcattt
    20701 atctctagtt ggagaggctt gtactttgag tagcttagca aatgagaata tagaaaaaaa
    20761 agagtattta aatgaaataa taaaaatatc aaaaaaatat ggagcatatg gagtaaatat
    20821 tgctcacagt ggaactgtag taggtatttt aattgataag tctatgaatg ataaaaagat
    20881 gatagatgcc ttatgtgaat ctaacataaa ttctgtctat aataaaattt atacacaaaa
    20941 cataattaat ggaggaatta agggggaaat agaatggaat acataaagaa tcctatgaaa
    21001 atagaagaaa aaagttttga attgatacaa gaaataatag acgaaattag accagattat
    21061 aaatttaaga ataatatcga agaaaaaata ataaaaagag ctatacatac tactgctgat
    21121 tttgattact tagatatatt aaaaatatca gaagaagcag ttgatagtat aatagatgca
    21181 ttaaaaaata atgcaagtat ttatactgat acaaatatgg ctcttagtgg tataaataaa
    21241 agaaagttag aatcattagg atgtaagtat aaatgtttag tggcagatga tgaaactgta
    21301 aaattagcta aagaaaaagg aattactcgt tctatggcag cagtagaaat tgcagctaaa
    21361 gaagaaggaa gaaaaatatt cgttttaggt aatgctccaa cagctttata taaagttatg
    21421 gagatgaaat cagaaggtag attagattta gatgcagtta taggagttcc tgtaggtttt
    21481 gtaggagcac aagaatcaaa agatgaagtt gaaaaaacag atataccata tataatttct
    21541 aaaggtagaa aaggtggcag taatttagca gcagcaatag taaatgctat attatatact
    21601 atgtaggtat ctatatggaa gaatatgtat atatagatgg aaaaaaatat agaagaggat
    21661 atactacagg ttcatgtgca acaggtgcat ctaaggctgc tgtatatatg ttgattacaa
    21721 aaaatagaat caatactata aatatagata ctcctaaggg gataccatta ctactaaaag
    21781 ttgataatat aaatatttct gatacatttg tggaatgttc cataaaaaaa gatggtggag
    21841 atgatataga tgcaactcat accatggata tttatgcaag agctgaaatt gtagctaaaa
    21901 atgataagaa caaagggtat cttactctaa aggatataga tagtttaagc accaatagtg
    21961 agtgtaaaag tgagctctat aagtttatta gggtttatgg cggaactggt attggtgtgg
    22021 ttacgaaaaa aggtcttagc gtggatgttg gtaaacctgc tataaatccg actccactta
    22081 aaatgataaa tcatgaaata agaaagctta taggagataa ttttgaatct atacttggaa
    22141 atgataaagt tttaaaaata acaatctttg ctccacaagg agaaactgta gcaaaaaaaa
    22201 catttaatcc aagattaggt atagttggag gaatatctat aataggtaca actgggattg
    22261 ttgaacctat gagtgatgat ggttggaaga agtctctatc tatagaactt cagatgaaaa
    22321 aagaacaagg cttagataaa ataatattgg ttccaggaaa tcatggagaa cagtttataa
    22381 gagaaaaatt aaacatagat attaagtatg tagttagggt aagtaatttt ataggctata
    22441 tgattaaaga agctcaaaga ataggatata aaaaaatctt aatggcaggt catataggta
    22501 aatttataaa agtatctgct ggtattttta atactcatag taaggtagca gatgctagaa
    22561 gtgagatatt agtggcaaac ttagctttaa tgggagctag atacgaattt ttaaataaaa
    22621 taaatcaatg cgtaactact gaagaagctg tagagctaat aaataatagt gaatatagag
    22681 aagtatataa tatattaagt aataaatgta gggagagagt aaagcaatac ttgaatgaag
    22741 attcagatga tattgatgtg gaagtaatca tattttcaat ggataagtct ttattaggta
    22801 aatcggataa tacagatgat ttagtggagg tctttatatg ataaacataa taggtctagg
    22861 acctggtaac ttagactata taacaaaaaa aggtgaaaat ttaatttcca cttctgatgt
    22921 attaattggt ggtaaaagaa atttagagtc tataaaaaat tttgaaggag aaaaaatagt
    22981 tctggattct aatttaagag aaattataga gtacataaat aacaataaag aaaagcaaat
    23041 atcaataata gcatctgggg acccattaat atatgggata ggtagatatc tatctaaaaa
    23101 tattgataat aagatgttaa acatggtttc tgggattagt tcattacagt acatattttc
    23161 aaaaatatat gtagatatga atgatgtata tattacaagt agtcatggca aagtaccaga
    23221 ttttgattac atattatcac ataaaaaagt atgtatggtt acagattcaa agataggacc
    23281 aaaacaaata agtagagaaa taatagatag aaatctaaat aaaattattg ttgtaggaga
    23341 aaatctgtca tatgacaatg aaaaaataac aatagcaaaa ccagaagaga taataagaat
    23401 ggacaatttt gatatgaatg ttgtggtgat tttagatgag gaatagtgag tttataacag
    23461 gtaaagttcc aattactaag gaagaggtta gagcaatatc aataagtaag ttaaatttag
    23521 taaatgctaa aaacctcata gacatagggg ctggtacagg aagtgtaagt gttgaagctg
    23581 catataatta tcctaattta aaggttatat ctatagagaa aaatgatgat gctatagagc
    23641 ttatagagaa aaataaaaaa aaattcaact taaaaaatat agaagtgata aaaggatatg
    23701 ctccaattaa tctagggtta aatgttaaag ttgagtctat ttttcttggg ggaacaggaa
    23761 ataatctgga agaaataata gaatggtcta aaaaaaatct cgttacagga ggaagacttg
    23821 tagctaattt tataataata gatacattta atcaaacttt aaaactgctt aaaaagcatg
    23881 gatttaaaga aatagatgta tgtgttttga atgtttctaa actggagaaa ctaggaaaag
    23941 gtgaatattt taaaccacta aatcctatat atataatatc ttgtgaaaaa ggggaaaatg
    24001 atgatgaata aagtacattt tgtaggagca ggacctggag ataaagagct gataacatta
    24061 aaaggataca aattattaag taatgcagat gtagtgatat atgcaggttc acttgttaat
    24121 cctgaacttt tggaatactg taaagaagat tgccaaatac ataatagtgc acatatggat
    24181 ttacaagaga taatagatgt tatgagagaa ggtatagaaa ataataaatc tgttgtaaga
    24241 ctgcaaacag gtgatttttc tatatatggt tcaattagag aacaagtgga agatttaaat
    24301 aaattgaata tagattatga ctgtactcct ggagtaagtt cttttttagg ggcagcatca
    24361 tctttagggg ttgaatatac agttccagaa atttctcaaa gtgtaattat aacaagaatg
    24421 gaaggcagaa ctccggttcc agagaaagaa tcaatacaat catatgctaa gcatcagaca
    24481 tctatggtaa tatttttatc tgttcaagaa attgagaaag ttgtgtctaa attactggaa
    24541 ggtggatatc caaaagatac tcctatagca gttatataca aagcaacttg ggcagatgaa
    24601 aaaatagtta aaggaacttt aagtgatata gctgtaaaag ttaaagaaaa caacataaat
    24661 aagactgctt taataatggt tgggagattt ttaggagaag agtataataa ttcaaagtta
    24721 tatgataagg atttcaaaca tgaatataga gggtaatatt gctataatct gtattacaga
    24781 aaatggaaaa aaattagctc taaagataaa ttctctagta aataactgtg ttatatatca
    24841 agtaaaaaat aaaaaaagta atttagttgt tgaagaaaca tgtgtacata cagttcaaaa
    24901 gaagttaagt gattttgtag gagaaatatt tagcaaattt gattacatag tatttataat
    24961 ggctacaggt atagttgtac gaagtatagc accatacata ataagtaagt ttagtgaccc
    25021 tgcaattttg gtaatggatg aaaaaggcaa aaacgtaata agtttattga gtggtcatat
    25081 tggaggggca aacagcttga cacagtacat aagtagtctt attgatgcta atccagtaat
    25141 aacaacagct actgatataa atcaaaaagc ttccttagat atgatagcaa aaaaactaaa
    25201 tgcacatatt gataatttta gagaaagtgt aaaagaagta aattctatgc tagtgaataa
    25261 taaatgtgtt ggattatata ttgatgaagg atatcaagtt gatactaggg gatttaaact
    25321 tatagaaagt aaggatgagt taagagatag tttttcaaac acatatatag aaaaattagt
    25381 tgtagtaagc aataaaataa attgtattga agatattgag aatttaaacc tagataataa
    25441 atttgaacat tgctatagta ccaaaaaaat aataaaggta attccaagag atatagtgat
    25501 aggaattggt tgtagaagaa acacagatag tgaactgatg tataattcac ttgaagagtt
    25561 tcttaataaa caaaatatag atattagagc aataaacaca gttggaagta tagaactgaa
    25621 aaaagacgaa aaagcaataa ttgaattatc aaaaatattg aaggtgccat ttaaaacatt
    25681 ttcagtagat gaaatatcta aggttgataa tctttttgat aaatctgatt ttgttaaaaa
    25741 aaatgtgggc gtgtattcag tagcagaacc tgtagcatat cttttgagca aaggtaattt
    25801 gttagttgaa aaacataaat ataaaggaat aactttttca gtggggagaa taaaaatatg
    25861 atatacgttg taggaattgg accaggaagt aaagatacaa tgactttaga agcaataaaa
    25921 gcaatagaag attctgaggc tattgttgga tataagactt atataaaact aatagaagaa
    25981 tttatacaag gtaaagaaat aatccaaaat ggtatgaggc aagaagttga tagatgtaaa
    26041 caagcagtag aggaagcaaa aaaaggaaaa aaagtagcag ttgtaagcag tggtgatgct
    26101 ggaatatatg ggatggcagg tcttatttta gaattaatat ctaaagaaga tgaagatata
    26161 aaagtaaagg taattccagg tgttactgca agtattggtg cagcagctat acttggagct
    26221 cctattatgc atgatttctg tcatataagt ttgagtgatt taatgactcc gtgggaagta
    26281 attgaaaaaa gattgagatt ggctgctgaa gccgattttg taatttgttt gtataatcca
    26341 agaagtaagg gaagaagtga acatctaagc aaagcattta aaataatggg tgagtttaaa
    26401 ggtggagata cacctgttgg agtggtaaaa gatgtaggta gagaaaaaga agaaaagttt
    26461 gtatgtactt ttgaaaatat ggactttgaa aaagtcgata tgactacaat ggttataata
    26521 ggaaataagt ctacctatat taatggtgaa ttgatgataa ctccaagagg ttatacagta
    26581 tgattttaat tttaggagga acttcagata gtttatctat atgtgataag ataaatgaac
    26641 tgaaaaatca accatatatc ctttcagtta ctacagatta tggaagggaa cttgcacaac
    26701 aacatgcaga gaatgtaatt ttaggtaagc ttggaaaaga agatatgcta aaatttataa
    26761 aagataataa tatagtaaaa ataatagatg ccactcatcc ttatgcagtt gaagtatcca
    26821 aaactgctat tacttgtgca aaactattga atatagatta tataaggttt gagagaaaat
    26881 ctttaattga agaaataaat tatgataata aatttatagt agatacaatt gaagaagcct
    26941 gtgaaatagc aaataaaata gggacaaata tattcatagg aacaggaagt aaaaatttga
    27001 acagatttat agaggaaata agtgataaaa atttagttgt tagagttctt ccaacgagtg
    27061 aagtcatatt aagttgtgaa aaattaggtt taagtgcaga taatatagta gctatgaaag
    27121 gtccatttag ccagtatata aatgaagaaa catataaaca ttacaatata gatttagtca
    27181 taacaaaaga aagtggaata gctggtgggt tcttagagaa ggtaaatgca tgtaaaactt
    27241 tcaatatacc agtaataatt ataaaaagag aatcgattaa ctacgaaaat gtatttaatg
    27301 acataaataa aattaaagac atacttgtgt aatttttagg aggagaatat atgaagaaag
    27361 ccttattagt agtgagtttt ggtacaagct atcatgaaac gagagaaaag acaatagata
    27421 tatgtgaaga taaaataaaa aattcgttaa aaagccatga tttttttaga gcttatacat
    27481 caaatatgat aataaataaa ataaaaaaaa gggatggaat agaaatagat aatccaatac
    27541 aggctttaga taaaatatat gaacaaggat atgatgaagt aataatccaa acacttcata
    27601 ttatatgtgg cgaagaattt aacaaattaa aagaacaagt agatggatat tcttctaaat
    27661 tcaaaaagat tgtattagga agacctttgc ttacacatat tggagattat caggaagctg
    27721 tagaggcaat taagcatcaa attcctcata tggaaagtaa tgaagcagta gtatttatgg
    27781 gacatggaac acttcatgaa tcacattcag cgtatccagc attggaatac atgcttaggg
    27841 ataatggaat aaatgcttat gttggtacag ttgaagggta tccagaaata gaacatgtaa
    27901 taagaagatt aaaagaagga aatataaaaa ctgtaaattt gatgccattt atgttagttg
    27961 caggagacca tgctataaat gatatggcag gagatgaaga agattcttgg aaaacaatat
    28021 tagaagaaaa tggatttaac gtaagagttc atttaaaagg actaggagaa aattcttata
    28081 tacaagataa atttgttcgt catgctgtaa aatgtgcaga gaatgcgaag ttctatggta
    28141 taggagcagg gccaggagat ggttctttac ttacgataaa agcggttaat actttaaaga
    28201 aactggatat tctatacact ccagaatcaa aaaagggtgg agatagtcta gctttatcaa
    28261 tagttagtga atatttacct gaatcagtgg aaataaagtc taggcatttc ccaatgagtt
    28321 ttgatgggaa tgaaaaaagt ttagcttggg acaatgtggc acaagaaatt atatcagatg
    28381 ttaatgatgg taaaaatgta ggatttgtta cattaggaga ccctatgatt tatagtacat
    28441 atgtatatat aatgaaaaga atattaggag aggtagaagt cgaaactata ccaggaattt
    28501 catcattttc caatatagca tctaatcaga attttccatt agtcatggat aaagaagctt
    28561 tagtggtaat tccttgtact atggaagatg ataagataga gtatgctttg caaaattata
    28621 attcaatagt attgatgaaa gtatataaaa attttaaaga aataataaaa aaattagaaa
    28681 agtatgattt aatagaacat gctattttag taagtaattc ttcccaagaa agcgaaaatg
    28741 tgtttacaga tttaaaagaa gcgcaccttg aagataagat atcttatttt tctactatac
    28801 tagtaaataa agataataaa ataaaatagg tgaaagcaat atttaaagga gacttaatat
    28861 gaatatagtg gttggaacta ggggtagtaa tctagcgctt atacaaacag aatgggtaat
    28921 aaatgaatta aaaaagaagt atccagaaat tagttttgag attaaaatca taaagacaaa
    28981 aggagattta attcaaaatg tttcactgga taaaataggt gataaaggat tgtttgtcaa
    29041 agaaatagag caacaattat tagatggaaa aatagacata gcagtacata gtatgaaaga
    29101 tatgccttct tatttagcag atggactaaa atttgctcat acgcctaaga gagaagaccc
    29161 aagagatgtt ctaatactta gagaaggata taaaaattta gatgatttgc ctcatggagc
    29221 agtaatagga acaggaagta aaagaaggaa gtttcaatta ttgaaacaaa gacctgattt
    29281 aaatatagta caagttagag gtaatgtgga aactaggata cgaaaaataa aagatgaaaa
    29341 catgcatggt attgtgttgg cagcatcagg aataattaga gctaatttac aagataaaat
    29401 aagttcttat ttacctgttg atgttgttat tccagcacct gcccaaggag ctttagctat
    29461 agaaattaga agcaatgata gtacgataga gggaatagta aattcactga aggatgagaa
    29521 tacagaaatc caaatattgg ctgaaagagg ttttttagat ggtgtaaatg gaagttgtca
    29581 tattccaatg gcagcttatt gtgaaataat ccaagataaa atacatctta ctggcttata
    29641 tggagattca gaaggaaaaa aagtagtaat caagtctata gatggagata tttcttctcc
    29701 tagagagtta ggtttaaagt tagctaaact tgtattgaag gagtatgaga attatgaagg
    29761 gtaaggttta cttagtaggg gcaggaccag gtgattataa actcatgact ttaaaaggtt
    29821 tagagtgtat aagaaaatcg gatgtaatag tatatgatag attagcaaat agtagttatt
    29881 taagagaagc taagcctgat tgtgaactta tatatgtagg caaggcatca agtaatcata
    29941 ttttgccaca agaagatata aatagaatta tagcagaaaa agctaaagaa ggaaaaattg
    30001 taactcgtct taaaggagga gacccttatg tatttggtag agggggagaa gaagcagaga
    30061 ctttagtaga tgaaggtata gaatttgaag tagttccagg tataacttct gctataggag
    30121 gtttatgtta tgcgggtatt cctataacac atagagacca tgcttcatca tttcatgtaa
    30181 taacaggtca tttgaagggt gatgatagtg gagaattaaa ctggaatgct cttgcaaata
    30241 acaaaggtac tcttgtgttt ttgatgggaa tttcaaacct agagaaaata agtgaaaact
    30301 taatgaaaga aggaaaagat aaggatacac ctgttgctct tataagttgg gcaactagat
    30361 ataatcaaag agttgttaca tctacacttg aaaatgttta tgaaactgct attaaagaag
    30421 aagtaaaacc gccaactctt atagtagttg ggagtgttgt aggacttaga gaaaagttga
    30481 acttctttga aagtaagcct ttatttggaa aaagtattgc tgtaactagg tctagaaatc
    30541 aaaatagtgt attggtagaa aaaataatgg atttaggtgg aaatcctata gaaataccta
    30601 ctattaaggt agaaaaaata caaaataata ttaacttaga aaatgaaata aaaaatataa
    30661 ataagtataa ttatctaata ctaactagta aaaatgccgt agaaatcttc tttgaaaaga
    30721 tatatgaaat gaattttgat ttaaggatat tatctaattt aaaggtatgt gctataggtt
    30781 ctgctacatc aaatgagttg aaaaaaagag gaattatagc ggatatagta ccaaaaaaat
    30841 ttgtagcaga gtcactgtat gaagagttag ctcctatttt aaatgatgac gataatatac
    30901 ttatacctag agcagaaaat gctagagatt atctggttga caaacttaag gctatttgtg
    30961 aagtaacaga agtacatata tatagaacag taatagatga ggagaaaaaa ggtgagatat
    31021 tagatatttt aaattctaat gacatagatt atataacatt tactagttcc tcaacagtaa
    31081 ataatcttgt tgaaattata ggaaaagaaa atattaaaat attagaaaaa ataaaaacac
    31141 tatctatagg tcctataact acagagacaa taaatagttt gggaataaaa ttgtacaaag
    31201 aatctgctac atctactata gattcgttag tagatttaat tataaaaaat taattttgga
    31261 ggcttgaata tgttaagaag accaagaaga ctaagagcaa gtaaagcaat cagaaatttg
    31321 gtaagagaaa ctaaggtgaa tgtagaagat ttaatatatc cactgtttgt agtagagggg
    31381 gaaaatataa aagaagaaat taaatcttta ccagatgtct atcatttatc aatagatatg
    31441 ctagaagacg aaataaaaga aataagtgag ttgggaattg aacatataat gttatttggt
    31501 gttccagatg aaaaagatga gtgtgcttca caagctttta atgataatgg aatagttcaa
    31561 aaggcaatta gaaaggtaaa agaaataaat tcagatataa atgtaataac agatgtctgt
    31621 atgtgccaat atacaagtca tggacattgt gggattttaa ctgatactgg ttatgttgat
    31681 aatgataaat cattggagta tttggctaaa atatctgtaa gccatgctaa agcaggagct
    31741 gatatggttg ctccatctga tatgatggat ggaagaatac aagcaataag agaagcttta
    31801 gatgaaaatg gattagaaaa tgttggaatc atggcttata gtgttaagta tgcttcaaat
    31861 tattatggac cttttagaga ggcagctcat tcagcaccat cttttggaga tagaaaaaca
    31921 tatcaaatgg acccagcaaa tagcaatgaa gctataatag agactgaact tgatatattg
    31981 gaaggtgcag atatacttat ggttaaacct gcattatcat atttagatat tataagaaga
    32041 gttaaagata attttgactt acctctagca gcttataatg taagtggaga atactctatg
    32101 cttaagtttg ctgtgaagga aggtttatta aatgaagatg taattcttga atcaattaca
    32161 tctataaaaa gagcaggagc agatataata ataacttatt ttgctaaaga cattgctaaa
    32221 ctgttgaaaa aataaattct agtagtataa agaatatcaa aaagtatagg aattgtaatt
    32281 aactattata cagtagtttt gtttagacat ttattgaaga ataatagaaa ctaatttgca
    32341 atttctatac tttatttatg taactaaata aaatttggag gtagaagatg ctatatccta
    32401 ttaatttaaa attagatgag ttagatgtag ttataattgg tggtggagaa gttgcatata
    32461 ggaaatgtaa aaatttttta gagtttaaca aaaatgttac tatagtttct aaacaaattt
    32521 taaataaatt ttatgattta aaaggaaata taaaaataat aaaagatgat tataaagaaa
    32581 tatatataaa agatgcttct gtaataattg ctgccacaaa taatagagag ttaaatatgg
    32641 aaataggatt atattgtaga gcaaaaaata agctagtgaa tgtagttgat aatatagaaa
    32701 tatcgaactt tactgttcca tcatatataa aaagaggaga tttacttata agtgtgtcta
    32761 ctgggggcaa gagtccttca ttatcatcaa agataaaaaa agaaattgaa gagagatata
    32821 ctgaagatta tgaggagtat ttaaatgtat taggagatat aagaaaaaag gttataaaaa
    32881 aatacgaaga taaaagtaaa agaaaagatg tgcttaatat gttggtaaca cttgacctag
    32941 aagaacttaa gaagtttgat atataaaaaa taaaataaaa aatgttaaaa aggtgttgac
    33001 ggattatttt taagatgata tagtattact tgtccttaag aaaagggcaa acataaaaaa
//
LOCUS       DAEMIK010000029        31226 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEMIK010000029 DAEMIK010000000
VERSION     DAEMIK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.029"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            97..2325
                     /locus_tag="KPK87_001160"
     CDS             97..2325
                     /locus_tag="KPK87_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG2537329.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2563..2862)
                     /locus_tag="KPK87_001161"
     CDS             complement(2563..2862)
                     /locus_tag="KPK87_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG2537330.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3068..6109
                     /locus_tag="KPK87_001162"
     CDS             3068..6109
                     /locus_tag="KPK87_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG2537331.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKGNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSSFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6270..7544)
                     /locus_tag="KPK87_001163"
     CDS             complement(6270..7544)
                     /locus_tag="KPK87_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2537332.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(7781..12862)
                     /locus_tag="KPK87_001164"
                     /pseudo
     CDS             complement(7781..12862)
                     /locus_tag="KPK87_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(12873..14405)
                     /gene="purH"
                     /locus_tag="KPK87_001165"
     CDS             complement(12873..14405)
                     /gene="purH"
                     /locus_tag="KPK87_001165"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG2537333.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKNHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(14398..14991)
                     /locus_tag="KPK87_001166"
     CDS             complement(14398..14991)
                     /locus_tag="KPK87_001166"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG2537334.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGI
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(14985..16061)
                     /locus_tag="KPK87_001167"
     CDS             complement(14985..16061)
                     /locus_tag="KPK87_001167"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG2537335.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVSGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(16055..17422)
                     /gene="purF"
                     /locus_tag="KPK87_001168"
     CDS             complement(16055..17422)
                     /gene="purF"
                     /locus_tag="KPK87_001168"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG2537336.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(17434..18132)
                     /locus_tag="KPK87_001169"
     CDS             complement(17434..18132)
                     /locus_tag="KPK87_001169"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG2537337.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(18138..18614)
                     /gene="purE"
                     /locus_tag="KPK87_001170"
     CDS             complement(18138..18614)
                     /gene="purE"
                     /locus_tag="KPK87_001170"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG2537338.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            19064..19576
                     /locus_tag="KPK87_001171"
     CDS             19064..19576
                     /locus_tag="KPK87_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2537339.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQILLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            complement(19960..21123)
                     /locus_tag="KPK87_001172"
     CDS             complement(19960..21123)
                     /locus_tag="KPK87_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2537340.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(21120..21518)
                     /gene="tnpA"
                     /locus_tag="KPK87_001173"
     CDS             complement(21120..21518)
                     /gene="tnpA"
                     /locus_tag="KPK87_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG2537341.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            21966..22181
                     /locus_tag="KPK87_001174"
     CDS             21966..22181
                     /locus_tag="KPK87_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537342.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            22187..22480
                     /locus_tag="KPK87_001175"
     CDS             22187..22480
                     /locus_tag="KPK87_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG2537343.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(22608..23375)
                     /locus_tag="KPK87_001176"
     CDS             complement(22608..23375)
                     /locus_tag="KPK87_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2537344.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDKVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSKKMLIIELYKIINNNYIKLYV"
     gene            complement(23463..24728)
                     /locus_tag="KPK87_001177"
     CDS             complement(23463..24728)
                     /locus_tag="KPK87_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG2537345.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            complement(24832..25140)
                     /locus_tag="KPK87_001178"
     CDS             complement(24832..25140)
                     /locus_tag="KPK87_001178"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG2537346.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            complement(25207..26262)
                     /locus_tag="KPK87_001179"
     CDS             complement(25207..26262)
                     /locus_tag="KPK87_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG2537347.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            complement(26264..26716)
                     /locus_tag="KPK87_001180"
     CDS             complement(26264..26716)
                     /locus_tag="KPK87_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2537348.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            complement(26713..28743)
                     /locus_tag="KPK87_001181"
     CDS             complement(26713..28743)
                     /locus_tag="KPK87_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2537349.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVYINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IAIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            complement(28999..>31226)
                     /locus_tag="KPK87_001182"
     CDS             complement(28999..>31226)
                     /locus_tag="KPK87_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2537350.1"
                     /translation="SRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSKNYL
                     NDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGIIDL
                     EGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSILIG
                     VSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEPWAQ
                     KMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNKLNS
                     FTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKPLIS
                     ESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHDADL
                     FYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLITIQD
                     HANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKIDIK
                     SGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWIKEG
                     KEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAIDIIE
                     DIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSIIEL
                     SKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIKNIH
                     Y"
BASE COUNT        10217 a         5408 c         3613 g        11988 t
ORIGIN      
        1 aagacaaaat tttaacttta ctatttgtgt aaaaagtaat ataatttaaa attgattata
       61 aatctaactt ttttctttat caaaatattt taggaggtga gacatttgaa aaaaactaat
      121 agtttaaaag aaattttaaa ctggaataat atcaagaatt taagaccttc aaagggttta
      181 aaaaatatat tagaaatttt taaaagtgac ctaaaagata tacgtggcaa ttatgccgta
      241 cttgtaatag ttttaggtct tgctttttta ccatctctgt atgcttggtt caatataaag
      301 gcatcttggg acccttatgg aagcactgct aatatgcttg tagcagtaac aaatgaagat
      361 aaaggaaatg aaatagcagg acaaaagatt aatgtaggag accaattaat tgaacaactc
      421 aaagataaca agagtttagg ttggcagttt gtagatgaaa aaactgctct tgaaggtgtt
      481 aaaaaaggta cttattatgc atcacttaag attccaaaaa attttacgaa agatttgaca
      541 tctttaatta cagatgatgt taaaaaaggg gaaattatct atactgttaa tgaaaaaata
      601 aatgcagttg caccaaagat tacagataaa ggtgcttcta caatacaact acaggttaat
      661 cagactatag taaaaacagt tagtgaaatc gttcttggta tatcaaacgg agttggtgca
      721 ggaattgaac acagtcttcc aaaattgaca aaagttgaaa attcccttat tgaccttcaa
      781 aagagatttg accatataaa taagactgtt gatttagctt ctgatgctac ttcaaaatta
      841 tctgatacag ttaaagatat taaatctgat ttacctacaa ttaaaaagac tctgaatgat
      901 acaaaactgt tatcaagtga cttaaaaaaa ttcttagagg atacaaatga taatttagac
      961 gaactttctc ctcttattaa gtctgactta aatttaatgg ttgacctttc atcttctgct
     1021 tcttctctta cattaaattt aattgatgca gtaaatagtg gcagtgaaga tgtacctaag
     1081 ttgatagata atttatcaga aaaactttct aatttgcaat ctttaaatga tactttggtg
     1141 gattttttaa ctaaattaaa ccaacttaca tcaaataata gattggatga tgtcatagat
     1201 aatcttgaag actctagcaa taaaatagat tcatctatat caacattaaa tgatattaaa
     1261 aacaaagtta tatctggaca acaaccatct ataagtgctt tgaataatgt tttaagtcta
     1321 tcaaatggta ttggaagaat taatttaaat attttaaata attttgactc aaaaatatca
     1381 aaacctataa atagtatatt tgcaaacagt ataaaagtag caaatgatat aattactgtt
     1441 ttagataagg cagaagcaaa acttccaaag gttgaagaaa tacttactac ttctctaaaa
     1501 ctttcaggaa atgcacagga aagtatatca ttgataagag aaagacttcc tttagcaaaa
     1561 ggaatgttgg atgatttgat tgatacttta agcaaaatta gtaatggtga agatatgaaa
     1621 aaattagtca gcttgcttga aagtgatata ttgacaaagt ctaatttctt aaaagaacct
     1681 gttgaggtga aaacagagaa attatatcca attgaaaatt atggttctgc aatgacacca
     1741 ttttatagtg ttctttctac atgggttggt atattactta tttcagcact attgtctaca
     1801 tctgtacatg gaaactataa accttatgaa atttactttg gaagaggcct tactttttta
     1861 agtatagctc ttgtccaagg ttttataata ggaactggag atatattatt gttaggagta
     1921 acagcaaaac atccaatttt attaatagtt ctgcttatgt ttactagtat ggtatttaac
     1981 tttatagtgt attctttagt ttctgtattt ggaaatgtag gaaaagcaat tgcaatagta
     2041 ctattagtcg ttcagattgg ttcttctggt ggtacattcc ctatacaagt tacacctaca
     2101 ttcttccaaa gaataaaccc tataatgcca tttacctatg caataggtgc tacaagggaa
     2161 gcagttggtg gtatatatgc tccaaatcta actaaagata tactagttct attactattt
     2221 atgattctgt ctatttgttt aaatgtactt ctaaaaggac ctataaatag agttataaaa
     2281 ccatttaatg ataaattcgg cgatagtaat ttaactggtc attaaaaaat taattctgtc
     2341 tttaaatata tttttttaat aatattctaa gttcatatta aaaggtagta gctaaaaagc
     2401 tactaccttt ttccaactta aaaactaata tttactacaa aaactgtcat agaaaaaaac
     2461 gaaaaaattc gttctttcct aaatttatct tataaatatt tagatatatt cctacttaat
     2521 aagcagagct atacatagta tttaatgtat aacttacatt atttaactca tataatcaat
     2581 tattcctgaa tttaaattat ataaatgctc aaaaccttct tcagctaaca aattacatgc
     2641 agtaacactt ctatgaccac ttctgcaata aactactact ttcttatctt tataatcgta
     2701 aatttcatct atattattga ttaactcttg taaagatata tttatagcat tttctatttt
     2761 atattcctta aattcatctt tagttcttac atctaataat aaaacatcct tttcattttt
     2821 catcatttct ttaagttgct ttttatttat attcttatac attgtctaaa attcctccat
     2881 tttatttata tattacctat accctttatt ctttatacaa caacaaatat tatttttata
     2941 tttttctctg ttatataaaa agctatactt ataaaagctt tttttattga taataatctg
     3001 ttgattaaaa taattaagaa tgttatgcta attataaatt attaaaaagg gaaagaggta
     3061 atgtaatatg tttaagcaaa ggctctctaa attattatct agtacattag tattgagtat
     3121 gttatttact gctgctccaa atattacttt tgctgataat acaaaaggta attccgaaaa
     3181 atatcaaagt tctgatatag aattacatga ttatagtaaa aacgcggaat cttacacaaa
     3241 aactaaagct cttgctaaag agaaaatcca aactttgcta tcaaagtatg gtgctgtaag
     3301 tgctcaatat gctttgattg ataatggcaa aattgagatt tctggtaatg gtggagttta
     3361 cagcaaacag gataacaaaa acttaaataa ggataatatg tatagtatcg catctatcag
     3421 taaaatgttt actacaacag ctgttatgaa gttggttgat gatggaaaat taaatttaga
     3481 tactcctgtt gtaaagtata tacctgaatt taaaatggct gatgatagat acaaggaaat
     3541 cactccaaga atgttattaa atcattcatc agggttgatg ggaagtagtt ttaaaaacac
     3601 tattttatta gcagacaatg attcttatgg tcatgataat ttcttaaaag aattacaaaa
     3661 acaaagatta aaagctaaac caggagcttt ttctgtttat tgtaatgatg gatttacttt
     3721 agctgaaatt ttagtagaaa gagtatctgg tatgtcattt actaattttc ttgataaata
     3781 cataaataat ccacttaact tacaaaatac aaaaactcca gaaaatagtt ttgatagttc
     3841 aaaactagca aaagcatacg taccttattg ggaagatgca gttccgcaag ataaccttaa
     3901 tgctataggt gcaggtggtt tatattcaag tgcagaaaat ctatgtacat ttgcacaaac
     3961 atttatgaaa aattcaaatg gtattctttc tcctgcttca gtaaaagcta tggaaaacaa
     4021 agaatattta aatggtctat ggcctgaagg agaagattct atacttggat atggtttagg
     4081 ttgggattgt gtcaacactt atccatttaa tcagtataat ttaaaagctc ttacaaaagg
     4141 tggagactct ttattattcc acagtaatct tatagttctt ccagatgaaa atatggcagt
     4201 tgcagttctt tcttctggtg gaagtagtca acttaacgaa ataattggac aagaaatctt
     4261 actatctgca ttaaaagaaa aaggtaaaat taaagaaata aaacctgata aaacatttag
     4321 taaaccacaa caagttaaaa tgcctagctc tctaaaagaa aatagtggtc tttatgcttc
     4381 ttcaaatatg ataaaagtag atgttaatga taatggtact ttaacagtat cttctcctta
     4441 tatagaaaat ggacctgaag ataaatacgt atacataggt caagatagat ttgtatctga
     4501 aaaaggtaac agctgtctaa aatttgtaaa agaaaaaaac aatatcacat atttaaatat
     4561 gagctcttat gatgatgtcc ctggattagg tcagacagca agtttatact atgtagctca
     4621 aaagatagat gacaacaata tatcaaatag tgtaaaagaa gcatggaaaa aaagaaatgg
     4681 taaagattac tatctagtag atgaaaaata tacttctcaa agctatatgt ttggttctgt
     4741 gaaggcaact cttgctcttt ctgatgaaac tccaggttat atagttaaca ctaaaataat
     4801 ggatgagaat aactcaaatg cttttataga aatacctggt gtaataggtc gtgatttatc
     4861 cgacattaaa cttcataaag aaaatggtac agagtattta tcttttggaa cactaacgta
     4921 tgttagtgaa gattctataa ctaatttgcc agcagaaaaa tcttttactt gtgaactaga
     4981 atccaatggt tatgcaaaat ggtataagat tggtgacgat atcgcaaata aaaaaataga
     5041 agttaatctt cctcaaaatt catcttttgc tgtttatgat gataaaggtg ttcctgtaaa
     5101 ctattcttta gttacaaaga acaatagagt tagattgcca aaaggaggcg taattgtttt
     5161 cttaggaagt ccaaatgcta gatttgaggt tacatatcaa gatgaagtaa atgcaagtgc
     5221 acttacagga actgatagat atgaaacttc tattaagata agtcaagcag gttgggaaaa
     5281 tgctgaaaat gcagttttaa taaacgattc agcaattgca gatgctttgg ctgcaacacc
     5341 atttgcatac aagaaaaacg ctcctatatt attgactgga agctctcaaa taaatgaaaa
     5401 aactttggct gaattaaaga gattaaaagt taaaaatgta tatgtagttg gtggagaggc
     5461 ttctataaat gaaaaatctt tagatactat aaaatcaaac aatatctctg tatctagaat
     5521 ttcaggaagt gatagatatc aaacttctat gaacattgct aaagaattga acaatattag
     5581 caatatatca aagatttcag tagttaatgg cgaaaaagga ttagccgatg cagtaagcat
     5641 tggagctgta tcagctcaaa atgatatgcc tataatactt actaatgaaa atagtaatat
     5701 aactgaaata aataacttgt ttaaaaacaa aaagattgat aaatcttatg taatcggtgg
     5761 agaatataca gtttctaaaa atatagaaag taaacttcaa aatccacaga gaatatcagg
     5821 tagcactaga aatgaaacta atgcaaaagt aataaaagaa ttttacaagg attctaagat
     5881 agacaattta tatgttgcta aaaatggaat gaataaacaa gatgatttaa tagacggatt
     5941 atctgttgga gttttggctg gaaaaactaa atctcctgta atgctagtag gaaactcact
     6001 agattataat caaaaagaac tatttaaaac tatgagattt aaatctgtaa cacaaattgg
     6061 tggaaatgga aatgaaaact catttaagca aataaaagaa atagcttaac ataactaaaa
     6121 aaggctgtat tcaagtaata ttaattgaat atagcctttt tttatttaag ttataagttt
     6181 tatatgtgta aaaattaaat ttaaatatta aatgataagt ttattaagat atagataagt
     6241 ctaaaacttt gcctttttta agttagtttc tatttatcat tcatacttaa caatttatct
     6301 aaatatactg actttaattg tttactggct aaaatctttt tagctggttc aagctttaat
     6361 atagctgaca gtatagctcc catagctcta tgactagcca gagcattatt atcaaaaatt
     6421 aaattttgta actgcccttt ttcaattgcc ataagaactg ctctatgtac tgaatttgct
     6481 ggagttatta ttttttcatc tctctttaat aacataatgc tatttttatg gcaatttcta
     6541 acacaaactc cacaacctaa acatctatcc tcatcaattt ttatatattc tttaccatct
     6601 tccttaacct tgcttatcgc atctattgga catgcagtta tacatttacc acactttaca
     6661 caattttcat ggtttatatt tggtataaaa cttgttgtct gtactggatg aagatttcca
     6721 aatcttttag ctgctaccat tgcttcacag caacatccac agcaattaca tataaacgta
     6781 acccctttcc taacattttc tccacattga actaagttat gttcatatgc ttgatgaagt
     6841 agttctttac attctatttt atccacacgt cttgcaaatt tattatttat taaagaattt
     6901 gctacattgt caaacgtcat acatatatcc atgggagcat cacaagcttt tccaacatgt
     6961 tgcattctat gtctacagta acacatactt atacctatat gagttgattc atcaattata
     7021 tgagtagccc tttcataatc taaaatacta acctcattat cattagttaa aacttcctct
     7081 tgaacgtata ctcttccaag cttcgtttct gttgaataaa ataaatcttt gataaaatct
     7141 tcttctacat tcatatattg atagtaaagc tctgccaaaa gtttttggtc tatatcgtgt
     7201 ctcgttctca tcatggcaaa ctcaaaaaaa ccagccattg gaggaggcat tatatatttc
     7261 ttccctttat tatcttctat atctagtatc agagccttac tagcaagttt atccaatact
     7321 ctatatgctt cactttcact tacactccaa attttagctg ctgtttttac tctgaaaggc
     7381 tttattggaa gctgagcaac taacttcgcc tcttgttctg tatatagtac atttagtatt
     7441 ttgtataatg tatcagatgg tggagcacct tgtggaaact tgtttattct ttcctctaaa
     7501 cttttataca tattcttcgc agttatatga gaaatagtaa tcaccttctt tttcttttgt
     7561 tttccaataa aatcaaatat tctttcttat tatatattct cttactttat ataataccaa
     7621 ttctttctaa atagttaaat acctctagta atttattatt agatttctta tataaataaa
     7681 aaagagatgt aatattaaat gattatcatc acattattac atctcttcaa atctaaatat
     7741 ctaaaatata tattctagct atcaattaaa catcaaagtt ttatctaaaa tacttaactc
     7801 cagattcaaa tatcttctgt tctttttccc ctatgatatt cttatatact tcctcaccaa
     7861 ttctttcaga atgtcccatt ttaccaaaca ctctaccatc agcactagtt ataccttcaa
     7921 cagcatagaa tgaaccattt ggattaaact caatatcata agtagcttca ttattaaagt
     7981 caacatattg agttgctact tgtccattag caattaattc tctcataaca tcttcactgg
     8041 caacaaactt accttctcca tgagaaattg ctatattatg aatatctcca acattagttt
     8101 gagctaacca aggagattta ttagatgaaa ttctagttct agcaatctta gcttgatggc
     8161 gaccaatatt attgtaagta agtgttggag cactttcact tggaactctt atttctccat
     8221 atggaactaa ccctaattta attaatactt ggaatccatt acagatacct aacattagtc
     8281 catctttttg agttaaaaat tcatttatag cttcttgcac tctaggattt ctaaatacag
     8341 tagctataaa tttaccagaa ccatctggct catcaccagc actaaatcca cctggtaaca
     8401 ttattatttg agaagatttt atttcgttta ctattgtatc tatagaatct tctatgtctt
     8461 tataagttaa attcttaaat actcttatac ttgcattagc tcctgctctt tcaaatgctc
     8521 ttgcagagtc atattcacag tttgttccag ggaatgctgg tataaatact cttggagtag
     8581 ctatacttaa tgatgatttt ttagcttctc cttgtgatat ataacttata gtttctatct
     8641 ttgattttac ttcttctgtt ttagttggga aaataggctc taaagtacta caatgtgctt
     8701 tatataattc atctaatgca agttcttcac ctttgataaa tatactagct tctttaactg
     8761 tacttccaag cacaacataa ttgtatcctt ctaacatact taaatcatca tttgctaatt
     8821 caagtactat atttccataa ctagcctcaa ataatttatc atctgtaaat gctttttcag
     8881 cctcttcact aaacttaaat cctattttat taccaaaagc cattttactt attgcttcac
     8941 ctacacctgc aaatcctaat gcatatgttg ataaaacttt atttccatgt ataagttctc
     9001 ttactttatc taagtttctc tttaattcct caaaatctac aacatcattt tctgccttgt
     9061 ttacacataa cattactact tttgagtctg cttttttaaa ttctggagat actactttct
     9121 tcgcatctac tgtgtcaaca gcaaatgaaa ctaatgttgg tggaacttca atatctttaa
     9181 atgtaccaga catactgtct tttccaccta tagcaggtat ctcaaattta ctttgtgcat
     9241 aaaatgcgcc taaaagtgct gagaatggtt taccccattt ttctggattg ttaccaagtt
     9301 tttcaaaata ctcttggaat gtaagtcttg ttgtactata atttcctcca atagcaacca
     9361 atttacacac agattctact actgcataca aggcaccatg gaatgggctc cattttccta
     9421 cctttggatt gtatccataa gtcattattg tagaagtatt agtttctcct ccaagtactg
     9481 gtatcttagc aaccatacct tgtgttggag tcgcttgata ctttcctcca aatggcataa
     9541 gtactgtatt accaccaatt gtattatcaa acatctctac taatcctttt tgagaacaaa
     9601 cattaaggtc agatagtact tttgtaaact tgtctttcat acttgcacac ttaacatctt
     9661 ttactatttc attagagcta aagaatgtat tttcttcatc tactttagta acatgaacat
     9721 cagtagtttg ttttactccg tttgtatcta aaaactctct atttatatct acaattgctt
     9781 ttccattcca gaacattctt aaatatcctg tatctgttac tgtagctaca tgcgtagctt
     9841 ctaagttttc ttctactgct aattgtataa atttatcttt attttctttt tttatggcta
     9901 ctgccatacg ctcttgtgac tctgaaattg caagctcagt tccatcaagt ccatcatatt
     9961 ttttaggaac taagtctaag tttatatcta agctatcagc aatctctcct atagcaacac
    10021 aaactccacc tgctccaaaa tcattacatc tttttatcat ttgagcaact tctttatttc
    10081 taaagaatct ttgtattttt ctttcgttag gagcatctcc tttttgaact tctgcactac
    10141 aagttaagat tgactcttca ctatgttctt tagatgaacc agttgctcct ccacagccat
    10201 ctcttcctgt ttttcctcca agaagaacga ttacatctcc tgcttcaggt ctttctctta
    10261 ctacattctc ttttggtgct gctgctataa ctgcacctat ttccattctc tttgcaacaa
    10321 agttttcatc atatacttct gcaacttgcc cagttgtaag acctatttgg ttaccatatg
    10381 aactatatcc atgagcagct tctgtagtta tttttctttg cataagtttt ccaggtaaag
    10441 tatcttctaa agtcgttctt gggtcagcac ttcctgtaac acgcattgct tgataaacat
    10501 aacttcttcc tgataatggg tctcttatag ctccacctaa acaagtagct gctccaccaa
    10561 atggttctat ttcagttgga tggttgtgag tttcattttt aaacattact agatattttt
    10621 ccatcttacc atcaacttca acatctacgt ttatactaca tgcatttatt tcttcacttt
    10681 catctaggtc atttaatttc ccatttttct ttaattcttt tactgcaaca gtggctatat
    10741 ccattaaaca tatatcttta tgtctgttca catatacatt gtctcttgag ttaagataca
    10801 tttgatacgc ttcttttact atatcattaa acttaccatc ttctatcttt acattttcta
    10861 tttcagtcat aaaagtagta tgtctacaat gatcagacca ataagtatct aatactttta
    10921 tttctgttat agttggattt ctcttttctg tatctttaaa gtatttttga acgtgttgta
    10981 agtctcctaa agtcattgct aatccttttt cacttacaaa tgtttttaat ccattttcat
    11041 ctaaatctat aaatccatct aatacttcaa ctgttgttgg tatttcagtt tccattttta
    11101 acgtttctgg tttctctaaa gatgcttctc tactatctac tggatttata cagtaatctt
    11161 ttattttaga aaactcttca tcagttattt tgcctgataa tatataaact tttgctgtat
    11221 taatagcagg tcttatccct tgatttacaa tttgtacaca ttgagctgcc caatcacctc
    11281 tttggtcgta ttgaccagga agaaattcta ttgcaaatac tctttcatca tttaattttg
    11341 gtatttcttc ataatatact acatctaaat ttggttcaga gaatattgtc tttgctgcat
    11401 tttcataaac ttcttctgat attccttcta catcatatct atttaatatt cttaaatttt
    11461 ctatattatc tatgtgtaaa ctttctacta agtctttttt taaagcttta gcctcaaggt
    11521 caaaaccttc tcttttttca actaatactc ttctaaccat tgagtcttta ttttcagtat
    11581 tcaacatacc ttatcctcca ttttagaaaa attatcttcc tatgtctgtt ctatagtgca
    11641 ttccttcaaa gtttatcttc tttatgtttt cataaacttt ttctgctgca tccttaactg
    11701 tagttgactt tgcagttata ccaattactc ttcctccatt ggtaaccaaa ttaccatcaa
    11761 acatcttagt gccactatgg aatactacta tatcatcatc caagttttca agaccagtta
    11821 taattttccc tttttcataa ctgtctggat accctcctga tgtaagcata acacatacag
    11881 cttcatcatc actatagttt atttctatat cttttaactt attatctagt atagcttcca
    11941 ttatctcatg taaatctgtt tctaatctaa ataaaacaga ttgtgtctca gggtctccaa
    12001 atcttacatt atattcaagt actttttctc catcttcagt tatcataaga ccaacaaata
    12061 aaataccttt aaaattaagt ccatcttttt tgaagccttc taaagttttt tctaatactg
    12121 tctcttttac tttatttgct aattcttctg tatatatttc actaggtgaa aaagttccca
    12181 ttcccccagt gtttggacct tgctcatagt tgtttacttt tttatggtct tttgcacttg
    12241 ccataggaac tattgtatca ttgtctacaa atgctagaat tgaagtttct attcctttta
    12301 aaaactcttc aattactatt ttatctccag cagctccaaa cttcttatct gacatcattt
    12361 cttttaaagt tgctatagca tcttctctat tttcaggtat tacaacacct tttccagcag
    12421 ctaatccgtc cgcttttatt actactggat atccaaaact atctatttct gatatagctt
    12481 cttctaagtt tgtatattct ttatattttg cagtaggtat gttatgtttt atcataaatt
    12541 cttttgaaaa agctttactt ccttcaagtt gagcacattt tttattaggt ccaaatactc
    12601 ttaatccttc tttttcaaac tcatctacta ttcccataac tagtggtact tctggtccta
    12661 ctattgtcag acctatttca ttttctttgg caaattttaa aagttcttct atattagtat
    12721 cacctatatt tacacattct gctacgtttg caattcctgc attacctggt gcacaataaa
    12781 ttttttctac atttttttct ttgcttagtt tccagcatat agcatgctct cttccgccac
    12841 cgcctacaac caatatcttc ataatatcct ccttagtgtt tgaagtgtct tattcctgta
    12901 aataccataa ccatatcata cttgtcacaa gcttctattg aatctttatc ttttatagag
    12961 ccacctggtt gaactactgc tgatattcca tattcatgag ctaatgttac acaatcatca
    13021 aatgggaaaa atgcgtctga cgctaaaaca gctcctgtaa aatctttatc tttgttattt
    13081 tcaagagcat tttttaatgc ccaaattctt gatgtttgtc cacaacctaa agctagagtt
    13141 tgtccatttt ttactattgc aattgcattt gatttcatat tttttactac tctcatacca
    13201 aattccatgt ccttaagttg tgcatctgtt ggtttttcct ttgttacatt ttcacttttt
    13261 tctgctaata tattgtttct atcttgtatc aataattttc catctaaata tttcatttca
    13321 tatggttgta aactattttc tattttagct aattttaata ttcttagatt tttcttttgt
    13381 ttaagtatct ctaaagcttc tggttcaaaa tcatatgcta ctactatttc taagaatatt
    13441 tcatttaatt tctctgctgt agctttatct atagtagaag ttattcctac tattcctcca
    13501 aatattgaaa ccttatccgc ttcataacat tttgtatatg cttcaaaagc atcttttcct
    13561 aaacctaccc cacatgaatt agcatgtttt atagctactg aaactacttc ttcactatct
    13621 ttaaattctc tcattacttc taaacatcca tgtaaatcat ttaagttgtt aaaagaaagt
    13681 tctttgccgc ctaattgctc ataatttaag attggatttt ttgcatttga ctgtgaatat
    13741 aagaaaccat tttgatgtgg attttctcca tatcttaaag tttgttcttt ttggaaagtt
    13801 aaatttaata tgtcaggata cgtatctcca acttctccag caaaataact agatattaac
    13861 gcatcatatc ttcctgtagt tgagaaagct ttatatgata atttttttct gtcttctaat
    13921 gtcattgtat ctgtttttaa tttttctatt atagaatcat aatcttttat atctactact
    13981 actattacat ccttatagtt tttggcagca gaacgtatca tagatggtcc accaatatct
    14041 atattttcaa ttatttcatc atgatttttt ccagctttta aagttccttc aaaatcatat
    14101 agatttacag ctactaaatc tattgaatgt attttgtgtt catttacagt ttctacatga
    14161 gattctttat ctcttttata aagtatacca ccatgtatat atggatttaa tgtttttact
    14221 cttccatcta atatttctgg aaaatttgta acatcttcaa cttgcattac attaacacca
    14281 ttttctttta atgttttaaa tgtatttcct gtagatataa tttcataatc taatttattt
    14341 aactctttag caaattccac tacaccagtc ttgtcagtta cacttattaa agctctctta
    14401 ctcatttata aacactctcc taccttgaag ttttaattta ttttcacaaa aaagacttat
    14461 actttctttt aaaatcctat gttcaacttc taacactctt tttgcaagtg tctttgcatc
    14521 atcatcttga tttaccttaa ctacatcttg cattatgatt ggtccagtat cagcaccttc
    14581 atcaacaaaa tgaactgtag caccagttac tttagctccg taatctataa caccttgatg
    14641 aactttttct ccatagaatc cagctccaca aaatgaagga attaatgatg gatgtatgtt
    14701 tatcattttg tttctaaact cattaaccag ctttgggctg attattttta aataaccagc
    14761 caaaactact aaatctattt tattttcctt aagaatttct attatcttgt cttcatcagt
    14821 ttcacaaatt gcttttatgt tatgattttt agctctctct atcccataag caccttgctt
    14881 acttgagata actactttaa cctgaccttt tatttctcca gattcagtac catctataac
    14941 tgcttgtaaa tttgttcctc caccagatat taaaactcct atatttagca taattctaca
    15001 ccttcatgac tatctactac ttcaccaatt atataagctt tatcttctaa taattcacta
    15061 tattttttat ctacataatc agcattgtca ttttctctat tatttataaa gtttacaaca
    15121 tcatcagcat tttctttatc tactattaaa actagcccaa tacccatgtt aaaactctta
    15181 tgaagttctc tctcatcaac agcattaaaa ccttctatca ttttaaatat aggaggcttc
    15241 tcccaagatt ttttattgat atctagtcct aaccctttag gtataactct tgttatattt
    15301 tctataagcc ctccaccagt tatgtgagct atagctttta tgtcatgttt agctaataca
    15361 tctaaagcta atttaacata tattttagtt ggtgttaaaa gtgcttctcc aactgtcatt
    15421 cctagttctt caacatattg ttccatttta taattgtaag tttctaaaaa tatctttctt
    15481 ataaaagaaa atccattgct gtgtactcca cttgaagata taccaaccaa tacatctcct
    15541 gatttaacat tgtttccaga aacaatcttt tctttgtctg ctattcctac agaaaatcct
    15601 gctaaatcat agtcatcttc tccatacatt ccaggcattt cagcagtttc tccaccaatt
    15661 agtgcacaac cagacatttt acaaccatct gcaattccac taactatttt ttcgatatgc
    15721 tcaggcacta atttcccaag agctatatag tcaaggaaga ataatggttt tgctccttga
    15781 caaattaaat cattcacgca cattgcaact aaatctattc caacagtatc atgcttgtcc
    15841 atcatctgag ctatttttaa cttagtccca acaccatctg tagatgcaag caaaactggt
    15901 tctttcatac ctacaaaatc ttttaaactg tataatccac tgaaattacc taaatctcca
    15961 actacatttc catcataagt tccttttatt ttacctttta taaggtctac tgctctattt
    16021 ccttcatcta tatctacacc tgattcttta tatgttaaca tgaaagtagt tcctccttat
    16081 caattttctt aactgggtat tctccatcaa aacaagcttt acagaaagta gacttatgtt
    16141 ctgttgcatc taacattcct tctatatcta ggaattttaa actatcacat ccaatatatt
    16201 ctctcatttc ttctacgttg ttacttgatg caattaattt agagcgattt ggagtatcta
    16261 ttccatagta acaagagtaa gctactggag gagaagttat tctaagatga atttcttttg
    16321 ctcctgcttc tcttaaagat tttactaatt gtttagatgt agttcctctt acaattgagt
    16381 cgtctactaa aattatagat tttcctttta ttattgttga aagaggattt aattttattt
    16441 ttactgctat ttctctttct tcttgtgtag gttttataaa tgttcttcct acatatctat
    16501 ttttaactaa gccttcactt atttttaatc cactagcatt tgcataacca atagctcctg
    16561 gccaacctga atctggaact ggaactacca tatctgcctt tacagtttca ttctgagcta
    16621 atctctcacc acatttaatt ctaaattcat atgcatttac attgtctata gttgcatcat
    16681 ttcttgcaaa gtatatgtgt tcaaatatac aacttttctt aactggttta taattttctg
    16741 aataaaaata tgattttagt tctccatctt tgactactat tatctctcct ggctctacat
    16801 ctcttataac ttctccgcca agtatatcta ttgcacaatt ttctgatgca aatatatatt
    16861 catcaccctt tttaccaagt acaagaggtc tgaatccatg tgggtctcta actgcaacca
    16921 attcttcttg actcattatt acaagagcat atgcaccttt tatataatcc attgtcactt
    16981 ttaaactttc tactatatcc cctttgtaat atctagcaag tatatataaa atcacttctg
    17041 tatctgaatt agtttggaac atatatccat cttcttctag catatctttt aaataatttg
    17101 cattaactag atttccatta tgagctatgg ctaattgtct ttttctacaa cttccaacta
    17161 aaggttgaca gttagaaaca tgacttcctc cagctgttga atatctaaca tgtccaatcc
    17221 ctatatttcc ttttaactta gataattcat tttccttaaa aacatctcca actaatccca
    17281 tatctttttt gtacttaatt tctccatcat ctaaaagagc taaaccacag ctttcttgcc
    17341 ccctgtgttg catagaatat aaagaataat atagttcttt agttacatct ttattagagt
    17401 aaatccctaa aactccacac atacatttgc ctcctattta ttattcattc ttgaaagtac
    17461 ttccgtataa ccttctacaa ggtctccaag gtcttttcta aatctgtcct tatctaactt
    17521 ttcatttgta tttacatccc aaagtctaca agtgtctggt gatacttcat ctgccaatat
    17581 tatatttcct tcagaatcct taccaaattc tattttaaag tcaactaact ttaggtttaa
    17641 ttttaagaaa aatgctttta gtaactcgtt tatttttaaa gtttcttctc ttaagaattt
    17701 taactcttct ctagttgcta acttcattgc cactgcataa tcatcattta gcattgggtc
    17761 accataagca tcatttttgt agcttatttc aaaaattggc tcatcgaaaa ctacaccttc
    17821 ttcaagacca actcttttac agatagaacc agcagcaata tttcttacta ttacttctaa
    17881 tggtaaaatc tctacttttt taactaacat ttctctatct gataaacttt ttataaagtg
    17941 agtatttata ttattttctt ttaacatttc aaagattatt gttgatatct tattatttag
    18001 tatacctttt gaagatattt ctgctttttt ttctccatta aatgctgttg catcatcttt
    18061 ataataaaca acatattcat tttcattatc tgtagaatat acttgttttg cttttccttc
    18121 atataataac atttctgcta cccccttaaa ttcttgtcgt cttctaacac cttttgtgcc
    18181 atctcttctc tatatgattt caaatcctct tttaattttg ggtattttaa agtcattatt
    18241 tgtaatgcca ataaagctgc gtttaaacct aagtctattg taactgttgc aactgggatt
    18301 ccttttggca tttgaactat tgataaaagt gagtctagtc catccatagt tgatgattta
    18361 ataggtaatc ctattacagg aattaatgtt tgtgaagcta ttacacctgg taagtgtgct
    18421 gcttttcctg ctgctgctat tattacatca gtatcatctt ctatttcttt taggaatatt
    18481 gaaagttgtt ctggtgttct atgagctgat aatgctcttg ctacaacttc tattccatat
    18541 ttttcaagca gttttatccc ttcttctaat tttggataat ctgattttga acccataact
    18601 actgctactt tcattagact tcccctctcc gtttataagt tattttacac tacaaatcat
    18661 tcgtattatt atacgaatta tacaccttaa attttatatt aatatacata tattatcaac
    18721 tcttttttga aattttttaa aaaaaatatt tcttgtaatt ttttcattcg ctatgtatcg
    18781 aactttattt atgttttttt gttaaaataa aaaacagatt atctcaaaat tggttttcta
    18841 attaaatttt aagcccaata ctctttttta aatcttgtat attatttttt attacttctt
    18901 ttttaattaa tactttggat ttatagtctt gttttttagt tctgaatgtt acttttactt
    18961 agataaaacc aacacaaagt ttattcttta tataagtaat agctaaaatt taaagtaatg
    19021 atattatata aatagaaata attttattta aaggagaatc tgtatgttag aagttataaa
    19081 aaatagacgt tcaataagaa catatataga taaaaatata gaagaagata aaataactga
    19141 aatactaaaa tcagctatgc aagcaccttc atcaaaaaat gctcaacctt gggaatttat
    19201 tatagtagat gataaggaac tgctgaaaga gctctcaaag tcacagcata gagcaaagca
    19261 tatagaattt gcaccattat gtatagtagt gcttggaaat agagataaat tcttaaaacc
    19321 tggtaaatgg atacaagatt taggtgcttg cacacaaatt ttattattag aagttactaa
    19381 tcaaggtcta gctgcttgtt gggcaggagt atttcctaaa aataaagtgg taaataaagt
    19441 tagacaaact ttagacttac cattaaaatt agttccttat gcacttatat ctattggata
    19501 tagtgaagaa aaaaatgagt ttatagaccg atttgatgaa aataaaatac atagaaatgt
    19561 atataaaaat agataatgtt tagtaatata aatgaaaata catagtaaaa atgcctttag
    19621 aatacatttg tattctaaag gcattaactt tgactaaaca cctaattagc ctctaaggta
    19681 aaatttaact atcatttatt atttatatac caattgaata ttatatcctt caatattttt
    19741 atttatttca aatatattaa gaatatccta caattaaagt tagctatttt tcattaaagt
    19801 attttattga cacactcccc atagctaaag caaggggatt cttggtggct aacacactta
    19861 tttatactta aacagtacaa cagaggtggt tacaaagcca agctacttcg gacggtgtct
    19921 tcgccactac taccttaaag ttattcgccc acacatggct caaacggtag atacttttaa
    19981 atttattggt tgcttatgat ttattctaaa atctactaaa ccatatttat gctttttatt
    20041 taaaatattc caagcaccaa ctatatctct atgagtttca taaccacaag tacaactata
    20101 agttctacca gttggtttgt gttttttaga acactttggg caagtttgag aagtgtagtt
    20161 ttctttaact aacttaactt caataccttg ctcttttgct ttatatttta tatagtcctt
    20221 tattcgacca tactccattt gagatatttt ctgtctatta actctgttta atcttttctt
    20281 tttcttagta tctttttcaa ccccttttat atccccaaga actatttcac ataccttctg
    20341 ctctacacaa aagtctataa actttctagt tgtttgatga tataaattca ttaactgatt
    20401 atctgtttta cttcttatct tattttttaa tctccataac ttctttgatt gtctactacc
    20461 ttttttacat ttacttaatc ttttagatat ataagcatac atcttatttc taaattgttt
    20521 aatacttctg ccttctctat tagatagaat aagccctaca ccttcatttg ttacactagc
    20581 tatcgaatgt atttccccta agtctacacc tacggtatta gtgcctttgg ctacaactcc
    20641 atttaattca ttgtcaaaag tgtagtataa ataatatccg tttcgccata ctatttcagc
    20701 agtttttata ttctcaacat taaaatctaa ttctaaatac ctgccttttg acattgataa
    20761 ttttaagttg cctttagcat ttctattaat agcactagct ttaaaaggta ttatataaaa
    20821 tttcttagtc ttgtatggat attttgcttt agtattacct ttttttctta attctgatat
    20881 agtctttcta tttgcatcaa atttatctga tatagcttga atagtttgac tatgaagatt
    20941 gtacttacct ttgattaact tttgtatatc attcttttta atccattgtt tatgttcaaa
    21001 atagtattct ttagataagt taactatgta gttccaacag ttagcacttt ccatttgctt
    21061 ttcccttact aattcaaatt gtgttctagt taggtttaat ttcactctat gagtttttat
    21121 catttctttg tcctaacttg ttgattttct atatattgtt taactacttc taaactcaca
    21181 gaaccaacag tacctacaaa atagctatta gtccacatgc taggcatact agactttaaa
    21241 tgtggaaagt tctctcttaa tactctcgaa ctaaagcctt ttaaaccttt tactacttta
    21301 tgaattccat attgagggtc acaagatatt aataagtgca catgttcttt atcagtttcc
    21361 atttctatta tttctgctcc aagttcatct gctttatatg gtagtatttc ttttaacatc
    21421 ttttcaactt cgccaactaa aacaggttta cgatacttag ggcaaaatac aatatgatat
    21481 ttgcaatcat aaactatatt tttgttagat ttatacataa aaactaacac ctcctaaata
    21541 tagggtatca gtagcggtat tagattacaa gcattattct aacttttttt atattaagac
    21601 cttacctttc atccctacac ctaaaggaga gggcttttcg gcaattttat tgtaaattaa
    21661 taatgataat cacaacttta agaaaaatat agcccttaat gattatcata aaaattaatt
    21721 ttttgaaaaa actcatattg actcttgaag aagtacatac aaataaattt tgctttatga
    21781 atattcaaat aatttatttt atagatattt aatgataaat atcctacaaa attatattaa
    21841 tatattaggt cgactaaatt ttgatataaa gagaatataa aatactgtaa aaaagtgtaa
    21901 tggttatttc cttgaaaaga aagacatact attaaagcat aaattacaat ataggagatg
    21961 attaaatgag aatactttca gaaactgaaa aaatatcttt agcagcaatt ataaaaatgg
    22021 agtctgacgg attacttatg caaagagcaa taaatatgtt aatatcagat gaagacctaa
    22081 aaagacaatc agaatctagt atattagcaa cagaaggaag aattaaagct atacaacaat
    22141 ttatagttga aaatgaaata ctaatttctg aggaggtata gataacatga gaaatatgat
    22201 agaaaatcta attaagaaca atacttatat agacgataaa gtaataatgt taagtatgct
    22261 ttcatctgct aaagcatctg ccaatatgta tcttaattct gcattgacaa gtagtacacc
    22321 tgaattaaga gctatatatt cagcttcatt aactcaaatg gtagaaggtc acacagcact
    22381 tacagaatta agtattaata aaggatgggt taaaccatat gataaaccta ttcaacagtt
    22441 aacatgtagt tataaagaat cccaaaatgt tattgactaa actaaataaa atatatttct
    22501 aactatttca ttttaattaa ataataaaaa ggggatattc aaattttttt aagatttccc
    22561 cttcttctaa ataatctatt tttaacataa aatagattgc tcacaatcta aacatataat
    22621 tttatatagt tattattaat tattttatac aattctatta ttaacatttt tttactacat
    22681 atttttataa ataaagatat caaatcctta tgttccaaaa ttacaaaatc aggtttataa
    22741 aacaattctt ctacctcatg tcctctatgg ttcaaccata tactcttcca accagctttt
    22801 ttagcaccta atacatcatt tctataagaa tcacccacat aatatgtatt ttctctatct
    22861 aaattcataa cattctcagc tactctaaaa atattagtat ctggcttaga aaatccaacc
    22921 tcactagaaa taaaaatatt acttttatca acccagtttt caatatttaa ctgctttaac
    22981 ttcattcttt gatggtttga tgggccatta gttataattc ctagattaat atttctttct
    23041 ttagaaaaat ttaaaatcct ctcaacttct ggtattagtg taatttgtga ttgttgatat
    23101 atatactcat cttgaaaact ttgtgcatct ttttctgtaa tagaatttcc caactcctca
    23161 aaagctttca ttatcctata aatatgcatt tctttgattg gcatttctcc attttcagtc
    23221 atatgaaata ccttatcact atactttcta ctactcatat ataaatcttc aatagaaata
    23281 tcctttattg aagaaaacac tttattatat gcagtgtaaa aaggcgttaa ttgattatat
    23341 aaagtatcat ctacatcaaa aattaaatta atcaaaatat tagtcctcct aaatttaata
    23401 gtggtaagta taattataac tataaatacc tagtataata atactcacca ctataagtcc
    23461 tattatttat ttaaaatcct cgtagtaaca ccttttaagt tatagcattc tgcataatca
    23521 cgcaaaggac gtttaatatg gtctacaaca aatctttgac catcaatatt attcttactt
    23581 aaattatcat aaagcacttc tatttctttt ttaacatcat cattattaaa tgtataatgt
    23641 cctgcaatat ctagtatttc attagataaa acttcatcct taaatatctc ttcagtagtt
    23701 agattttttt gttctcctac tacccatttt ctccaacgtc cacattcaat tgcattaaca
    23761 agaagaacat gtctgatatt agaagttttc tcaactaagc cctctttttc taatttacac
    23821 tcaacttctg ctaattttaa atatgcccta gtttcttcag taccatattg aggagctaca
    23881 tttgtagcaa taatttgaga tggtatatgt tctaataaag ttacatcatc taagtaatcg
    23941 ccattatgct cttttaaacc aactccatat ttcttagcca tttgagctaa atcataagca
    24001 tttctaaaat taaaagttcc tacttgttct gtctttctag ttaaagtacc agtttgacca
    24061 actataaatg ttggcattgg caaacctcta ttatcaagtt caacctttag tctagttata
    24121 aatgtttcat acgtctcagt tgaagtcaga cctccattgg tttcctctgt accaacctca
    24181 taacctatgt ctggtagatt aagtcttttt ctctcgtttt cacaatactc aattaattct
    24241 acagttcttt ctagtactac atctagtgga attacttttc caatttcaaa tgggtctttt
    24301 gtagggtcaa tcatcaatag gtcaaaacct gcttcaatgt ccgctaaata agattttttt
    24361 cctaactcca ttgcttcatc aacaggcaaa tggtcatttc tctctttatc tctttgccat
    24421 ggcccaccat ggtctctaca taaataatat aaaccatcaa atccaacttt gtcggcaacc
    24481 ttcttaatat cttttgtaaa agtttcctga ttccaaccat ttacataacc gccacctagt
    24541 tcatctgcat ctacttgatt tctacttgcg ataaacatta aaggaaaatc atcatctttt
    24601 gctagttcaa aacttgcttg taacaagttt ggtgacatag gaccaattcc taaaagagtt
    24661 gcacttttcc cctcttcttg taagtttagt aatctttgca cgacagtctt tattggtaac
    24721 tttttcatga ctatatctcc ttttattcta tttttaagat attttcaaag atgcttttaa
    24781 taaaaatttc tttaaaaaca tctttgagtt atatgtaaaa taaatcaatt tttattgaaa
    24841 agtatctttt attttcttaa ttaatgcatc agatttctta attatctcag atacactcat
    24901 tcttactacc ttctttcctt taaaacgttc catatttcta atctcaacat cattagtcaa
    24961 tattacacaa gttgcttcat caatatcttt aagtgacaat tcattatcta ttccagagct
    25021 tccttgagtc tcaaccttta tttctatacc aaattttttg gctgataatt caagtgattc
    25081 agccgacatg taagtatgtg ctactccact aggacaagat gttactgcta caattttcat
    25141 attcttatct cctttaaatt tatagtttta tatttacttt tttatacaaa ttccttccat
    25201 ttatatttat aattcttcaa atattaattc aattgattca tctaaatctt cttcaatttc
    25261 ttcatcttct ttcacgtctt tttttactaa tttcattaat accgcaacaa ctactgctcc
    25321 agttaatact gatgcaatat accccaacct attgtcaact acaggtaaca ctatcagacc
    25381 tccccagggt gcatgattta aacatcctaa taaaaaagcc ataatatttc ctacaatcga
    25441 cccagccata atacaaggta ttactctaac tggatctgat gttgcaaatg gaatagctcc
    25501 ttctgtaatt ccaacacatc ccattaatag tgaagcttta cctgaatctc tttcttcctt
    25561 gctaaatttt tttggaaaca ataatgtagc taatcctaaa ccaattggag gtatacaaat
    25621 tgcaacgcca actccaccca ttaaatatgg aagtgtgtca acttgagttt gggcaaaagt
    25681 agcagcaacc ttatttatag ggccacccat atctgaggct atcattcctc ctaagattcc
    25741 acctaaagga attttagaaa caccagacat tccatttaac cagtttgtca tccatgtcat
    25801 aaaagaagct ataggttctc caattaaaaa tacaattata ccacctgtaa tcaatgtacc
    25861 aactaaagga taaataaata tagaacctaa agttctcatt gtaggagcta atttaatttt
    25921 ctttaattgt aaaacaacaa atcctgctat aaatcctgct atgataccac ctataaaacc
    25981 tgcatttact tcttttgcta aatatgcacc tatcatacct ggagctaatc ctggtttatc
    26041 tgccatacta aatgctatat atccacctaa tataggaatg aataaagcca agccagcagc
    26101 tccaatttga ttcagtttag ccaaccagcc tgtttcagga acagcagctt gtccacttaa
    26161 agttactgat aaagcaaata atattcctcc tgcaaccaca aatggaatca tataagatgt
    26221 tgctgtcata agatgttttt gcaaaaactt taaaattctc attttaatct tcctcctcta
    26281 ttttttcttt tataagatta taaacagttt gttcatctga acatctaatt tgatttctaa
    26341 aactttcatg catcaataaa cgtgctaatt ttgataaaat ctttaaatga agattatttt
    26401 cattgtcttt tgttactgat atcaaaaata ttaaatccac tttttctcct tcttctgtcc
    26461 attcaatagg agtatcaagc tttcctattg taatacaaga gtttttaaca cctacacttt
    26521 gactatgagg aagacctata ccatgtccaa tataagtcga caatgtttct tctctcttta
    26581 aaacatcttt taaaaattga tttttatcta ttaaatatcc ttcttttgac actacatcaa
    26641 ctaacgatat aatggcttct tcttttgaat taacatgtat tttaaatgaa cattttacat
    26701 taactaattt attcatacag tgacctcctt ctcatattta tgaattaaaa cttctgcttc
    26761 ttcttttgtc tttgcacttt ctaacatagc aataaaatta gtcttataaa ccattttagt
    26821 tagattaatt attgcaggaa tttgctcttt tttatctctg cttgctaggc aaaatatgat
    26881 tttaaccttt ttatctccaa aactaactgc ttctttacat attaataaac ttatcgaact
    26941 tactttgact aatgaactat cattgccatg tagtaaagca aattcgttat cttttacagc
    27001 ataaaaaccc acatttttta aaatatctat aatattctca gcatattgtg ctgatacaaa
    27061 atcacaatta gcaagtatgt ttgaactaaa ttttacagca tcttcccatt tcatatctcc
    27121 atcaattatc cttatggtat caatccctat taaatcactt aaattaagtt gtctaaataa
    27181 tattctctca tcactgtatc caagttcttg acatataata cttaatactc ttttttgggc
    27241 atcttcttct aaaaagtcca accttttttt tattgacata taattagtta agacgttctt
    27301 tctttttatc cctttttctt caaacttcaa ataatcctct tcttgtaaaa atggatttat
    27361 ctcaataatt gttttaggca agtctattgc tatctttgat gttgttataa tgaagtcaat
    27421 actttcccat tctttataat cttgtaactt atatgatgga atactctcta ctatatttat
    27481 ctggtaatca tttactaaac tatcctttaa catagcaatt acaccatatc caaatccaca
    27541 aactaacaat atatttttgt agttattcct ctcatttcta tgtatacttc ctaaaaaatg
    27601 tattgccaaa agtattgttt cattttcatt ctttatttga cttaaaactc tgtttttttg
    27661 aatagaaacc ttagtagctt caagtacata ttgatattta tctggtatta catgaagtaa
    27721 ttcctcataa atctgtatat tctgcttgat tctctctatc aatggagcaa tatgattgag
    27781 taatccttta taaagaatca tatcttcttt aaagctaatc tctaatattt ctgacatatt
    27841 gtaaattagg tcttgaacta taatttctgt actaatcaaa tccatatcct cgtttaaact
    27901 tgcatattta tttgtaaaaa atacaaaact ctttaacaga aaaatttgtt ccttagtcaa
    27961 ctttttttga ataattgttt caaattcttg gtcaaactta ttttcaaaac acggctcact
    28021 taatgaagca gattctaaag gatgtttttc atctgaatgt atataccatg taaaaaggaa
    28081 aatgttcgac acataccatg taaaagattc atcatttaaa gtccatccca tttgcaataa
    28141 taaattcata gaccaattat atatatcaat caaataaatc cctttgaata ttttttcaat
    28201 tacattcact aaatacttct caaatacact taaatcagat ttttccttac caaaaagata
    28261 agaatacgat tttaatatgt tcaatctttg cttatatata atgtcttctt ctccacttat
    28321 tttaaaagat ttatcataag taatagttat ttggtttctt tctaattcac ttgacaaaac
    28381 attaaggtca tttttaattg aacttctaga aacctgaaat tctttactta atttttctaa
    28441 gtttagatta tctattttaa acattgatat aattttcata atagatactc gctcagttaa
    28501 ggaaaataca tattcgttat tatttatata tactacttgg aagtcatcag gaataattaa
    28561 cacacctttg ttcatctttt gtattactga cttgtttaat gaacgcaaaa caatatttaa
    28621 attatttata tcatatctaa cttttctttc ttctatacta agttgtttgc taatatcctt
    28681 gtaacttgtc ttattttttc cttttagaaa attcatgatg agtaacattc tcccacttat
    28741 catattttcc ctccttccaa aataattaga gttattaata tagcaaattt tatattatta
    28801 tgtatgtttc agtttttatc taagacagta actatctttc tatattcaga ataatacaat
    28861 aaagctttag ccacaactta caacatttga aaattatttt tacaaaatct gcaaatatat
    28921 ttttattaaa tatataaata aaaaaggcaa gttatatttt taattatata acttgcctaa
    28981 attttttatc ttgtacattt aataatggat atttttaatt ttattatttt catatgcaaa
    29041 attttcaaag tcagaaatta ataatggttt tgaaaaaaca taaccttgaa tataatcaca
    29101 tcccacattt tttaggaact caacttgttc taattcttca attccttcag cagttacttt
    29161 catacctagc cttttgctaa gttcaataat agacttaata actaccttac cacggtctat
    29221 attatttttg ctaacaaaga atgaacggtc taacttaatt atatcaactt taaaatcctt
    29281 taatatccct aatgatgagt acccaaatcc aaaatcgtct atagaacatt taaatccatt
    29341 agaatgaata tcctctatta tatctatagc ttctggattt tctaagaaaa tagattcagt
    29401 aagttctaaa tctatcagac atgttggtac attgtattta ttgcatatat gcttatattt
    29461 ttctaaaaac aatttatcat tcagatgttg acgtgataca ttaacagaaa ttctcaacac
    29521 ttctttacct tcttttatcc acttagaaat caatttgcat gtttcttcta gtatatataa
    29581 atctaactta catataaatc cacttttttc gaaaagagga ataaaatcac ttggataaat
    29641 tatgcccttt tctgaatttt tccaacgcac aagagactct gctccactaa tttttccact
    29701 ttttatatca attttaggtt gtaagcacat aaaaaattct ttattttcaa gggctttttc
    29761 cattaaattt gaaatttcct tttcagatat taatcgtttt ctttctatat cactataaaa
    29821 agaaaaatca ctttgatgag ttttattaaa gctttttctt gctgtatttg catggtcttg
    29881 tattgtaatt aaatcttcat tatgattttc aattgtgtaa atacctactg atagtcttaa
    29941 aaagtatgga ttttccctat ttttattaaa ataatttgtg tcagcttcta ttttatataa
    30001 taactccagg gcttctaaca ctggtctatt cttaataagc aaatagaaaa ggtctgcatc
    30061 atgacggcac acaaactctt cattttcatc aatgttttta tacaaaatat tgtaaagatg
    30121 ttttagtgtc atatttccct tttcactacc gaaacaatca tttattagct taaaatcttt
    30181 tatattcatt gctatcatca tataagtact atcaggtgat tctgaaatta aaggtttgac
    30241 tagcatacta aacttagttg aatttatacc atttgttata ctatctaaat atgcaatatt
    30301 ttctatttga tttacatatt ttcttcttat aatatataaa attaatgtta taattccaat
    30361 aatacatgta attataaatg tcattgttat tgtaaaagag tttaatttat taagttcacc
    30421 aagtacagca tcttcaggta ctatgagaag aaaatgccaa tcatttaaac ctcttgtaat
    30481 tggatgataa gttaataagc attgtacacc ttttgatgag gttattgtaa tattccctga
    30541 tttatcattt ttaaaatcct caagcatctt ttgtgcccaa ggttcatctc cagaactctt
    30601 atatttatgg ctaaggtcct ttattaattc agtatttttt cctatgaaaa gaggttttcc
    30661 cttactatta ataatttcaa tagttccttt tccattaaag ttatctatat taagaatctc
    30721 attcatactt tctttattgg atacacctat taaaatacta gatacttcat gattatcatt
    30781 aaatataggt acactataaa gaacaaaacc cattgactct attacttctg agtattcttt
    30841 accttcaatt ccttttttat aggaaacagt attctttaag tcaaactttt ttccacttaa
    30901 aaactcagct ttaccttcta aatctattat tccaacatca taatacttat aactagtctt
    30961 tcttttagat aaatactcat atcgactatc accttcatat tgcttaataa aatctgccat
    31021 tattcttaag tcggataaaa taaatttgat tttagaatca acattcatta caatctgtgt
    31081 agatacatca tttaaatagt ttttagattg tttattagat atattgtgta gttttacggt
    31141 attaaaaaca gtaattacaa ggattgaaat aataagtgaa cataaaatta tagacagaac
    31201 taaactattt ttcttatacc ttgata
//
LOCUS       DAEMIK010000030        31170 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEMIK010000030 DAEMIK010000000
VERSION     DAEMIK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.030"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..485
                     /locus_tag="KPK87_001183"
     CDS             39..485
                     /locus_tag="KPK87_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2537351.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            487..1581
                     /locus_tag="KPK87_001184"
     CDS             487..1581
                     /locus_tag="KPK87_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG2537352.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMTKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFTSEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1669..2199
                     /locus_tag="KPK87_001185"
     CDS             1669..2199
                     /locus_tag="KPK87_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2537353.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2526..2885
                     /locus_tag="KPK87_001186"
                     /pseudo
     CDS             2526..2885
                     /locus_tag="KPK87_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3136..4512)
                     /locus_tag="KPK87_001187"
     CDS             complement(3136..4512)
                     /locus_tag="KPK87_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537354.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNELTLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4780..5952
                     /locus_tag="KPK87_001188"
     CDS             4780..5952
                     /locus_tag="KPK87_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2537355.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            5966..6613
                     /locus_tag="KPK87_001189"
     CDS             5966..6613
                     /locus_tag="KPK87_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537356.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6615..7124
                     /locus_tag="KPK87_001190"
     CDS             6615..7124
                     /locus_tag="KPK87_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG2537357.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7489..8601)
                     /locus_tag="KPK87_001191"
     CDS             complement(7489..8601)
                     /locus_tag="KPK87_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG2537358.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8898..9164
                     /locus_tag="KPK87_001192"
     CDS             8898..9164
                     /locus_tag="KPK87_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBG2537359.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9409..11136
                     /locus_tag="KPK87_001193"
     CDS             9409..11136
                     /locus_tag="KPK87_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537360.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11136..12926
                     /locus_tag="KPK87_001194"
     CDS             11136..12926
                     /locus_tag="KPK87_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537361.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13088..13396)
                     /locus_tag="KPK87_001195"
     CDS             complement(13088..13396)
                     /locus_tag="KPK87_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537362.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13658..14206)
                     /locus_tag="KPK87_001196"
     CDS             complement(13658..14206)
                     /locus_tag="KPK87_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537363.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14309..14980)
                     /locus_tag="KPK87_001197"
     CDS             complement(14309..14980)
                     /locus_tag="KPK87_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2537364.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15376..16281
                     /gene="garR"
                     /locus_tag="KPK87_001198"
     CDS             15376..16281
                     /gene="garR"
                     /locus_tag="KPK87_001198"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG2537365.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            16417..17799
                     /locus_tag="KPK87_001199"
     CDS             16417..17799
                     /locus_tag="KPK87_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2537366.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            17871..19307
                     /locus_tag="KPK87_001200"
     CDS             17871..19307
                     /locus_tag="KPK87_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG2537367.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKYLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19493..20347)
                     /locus_tag="KPK87_001201"
     CDS             complement(19493..20347)
                     /locus_tag="KPK87_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537368.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20615..20938)
                     /locus_tag="KPK87_001202"
     CDS             complement(20615..20938)
                     /locus_tag="KPK87_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG2537369.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21141..21770
                     /locus_tag="KPK87_001203"
     CDS             21141..21770
                     /locus_tag="KPK87_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537370.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22082..22591
                     /gene="ruvC"
                     /locus_tag="KPK87_001204"
     CDS             22082..22591
                     /gene="ruvC"
                     /locus_tag="KPK87_001204"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG2537371.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTHAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            22584..23186
                     /gene="ruvA"
                     /locus_tag="KPK87_001205"
     CDS             22584..23186
                     /gene="ruvA"
                     /locus_tag="KPK87_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG2537372.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23293..24312
                     /gene="ruvB"
                     /locus_tag="KPK87_001206"
     CDS             23293..24312
                     /gene="ruvB"
                     /locus_tag="KPK87_001206"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG2537373.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            24630..25655
                     /gene="queA"
                     /locus_tag="KPK87_001207"
     CDS             24630..25655
                     /gene="queA"
                     /locus_tag="KPK87_001207"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG2537374.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            25667..26152
                     /locus_tag="KPK87_001208"
     CDS             25667..26152
                     /locus_tag="KPK87_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537375.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26231..27352
                     /gene="tgt"
                     /locus_tag="KPK87_001209"
     CDS             26231..27352
                     /gene="tgt"
                     /locus_tag="KPK87_001209"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG2537376.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27486..27779
                     /gene="yajC"
                     /locus_tag="KPK87_001210"
     CDS             27486..27779
                     /gene="yajC"
                     /locus_tag="KPK87_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG2537377.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            27837..28199
                     /locus_tag="KPK87_001211"
     CDS             27837..28199
                     /locus_tag="KPK87_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG2537378.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28265..28408
                     /gene="scfA"
                     /locus_tag="KPK87_001212"
     CDS             28265..28408
                     /gene="scfA"
                     /locus_tag="KPK87_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG2537379.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            29033..30928
                     /locus_tag="KPK87_001213"
     CDS             29033..30928
                     /locus_tag="KPK87_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG2537380.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT        11934 a         3547 c         5326 g        10363 t
ORIGIN      
        1 tagtcaaaca tattataatt atagaaggga aatttaagat ggattttaaa aatttacagt
       61 atgaaagttt gtcatatatt atttgtaact tgcaaaaaaa ctttaaactt tattgtgaaa
      121 aatgtttaaa accatataaa cttacgaatg gtctatattt ctatttaatt tatattaata
      181 aaaataggaa ttgttctttg aatgatgttt ctacagaatt tgaagttgat aaagctcata
      241 caacaagaac tatatctagg ttagaacaag atggatacat agaaaaaatc caaaacccta
      301 atgatagtag agcttttcag ttgagagtaa cagataaggg tgaggaagta ttaggggata
      361 taaaaaacat attttcaaag tgggataatc acataaaaaa agagttctcg gatacagaat
      421 ataaagaact ggtaaagaat ttacatgtgg taaaggatat aaaaacagct gtggaggaag
      481 aataggatgt attgtaattt attaaaacca attaaaattg gaaatttgga attaaaaaat
      541 agagtatctt ttgcacctac aagcatgggt cttaaattgg aagaaaaaat taaaaagttt
      601 tctgatattg ctaagtcagg agtagctctg attactctag gagatgtatc tattagacct
      661 agttttcata aggtagctat aagcctatcg gatgaagatg gagtcatgaa atacaagaaa
      721 attgtagatg aaatacacaa ttctggtgca aaggtatcag cacaactatt ttgttcagat
      781 tatgatgtta atttaattaa agatactatg aagatgggaa ttacttcaca tgatgagata
      841 aaaaaaataa tgaatgacgg agtaaaagat tacataacaa atatgaccaa agaagaaatt
      901 aaaaatatta taaatttatt taaagtaaca gcattgaatg caaaaaaagc aggttttgac
      961 atgattcaaa ttcatggtga taggcttgta ggaagttttt catcaagtat atttaataat
     1021 agaaatgatg aatatggtgg aacgtgtgat aatagaagta gatttacaag cgaaataatt
     1081 agtagtatcc gagatgaagt taaggatatt ccaatagact ataaatttgc aataagacaa
     1141 gaaaatcctc attatggaaa tgcaggagtt ttactttcgg aagttgagta ttttgttaaa
     1201 aaatttgaat ccctaggtgt aaacagtttt catgtaacac ttgcaaatca ttcaaaatta
     1261 gaagatacta taccaaccaa taatcatcct tactttaaag atgaaggctg ttttttatat
     1321 ttagctgatg aggtaaagaa acacacaaat cttccagtat gtggggttgg aaaattgtct
     1381 agcccagatt ttatagaaag tattatttca aataatagag tagatatggt ttctatgtca
     1441 agacaattac tagcagatag taattggtta cagaaagtaa aagatggaag agtagatgag
     1501 attaaaaagt gctgttattg taataagaag tgtgcagatg ctttacaaac acgctctcag
     1561 tttggatgta tactagatta gaatatcctt ataaaaataa ataatttgtt aaggttaata
     1621 tatttttggt gatttagata agtatttaga taaagaggag gagacatcat gaaaagttta
     1681 gttttatatt caagtttaac tggaaatact aaaaagattg cttatactat atatgatgaa
     1741 attcaagaag aaaaagacat taaagatgta aatgaattag ttgattatag tattgactat
     1801 gaaaattatg atatagtatt cttaggctat tgggtggaca agggaatttg tgataaaaat
     1861 tcaaaacaag tgcttgaaaa tattcacaat aaaaagatag cactatttgg aacaatggga
     1921 gcaagtaaaa aaggaagtta tggagctagt ataatagaaa agatagaatc aattatccca
     1981 aaagataatg aaatattagg ttcattcata tgtcaaggta agatagcaga aggattgaaa
     2041 gcaaaatata aagagatgct aaaactttct cctgacaacg aacatattcg ccaacaatta
     2101 aataaccatg aggaatctca aagtcatcca gatgaacaag aaatatatga agccagtatg
     2161 tttgcaaaaa atatgatgat taaagctagt attgtgtagt agttaaacaa taagttcata
     2221 taagatatag aataaataat ttagtaaaaa agtatagcaa ataatttgct atgctttttt
     2281 tatgaaatca aaaaaataat aaaaatactt gactgtaaac cttgtttata cattatttta
     2341 tagaaaataa gatagtttta aatataaaaa aagatatatt attgaaagga gatattattg
     2401 tatgaaaact tataaaagat aattagtttg attttaagat atactaaata tacaatttta
     2461 tagagtgaaa atatttaaga ctatattata gttataaatt aatagaatag agaggtagtg
     2521 tttaaattgc aataacagta tttccaaagt tgccacctca aattcctatc caatggaata
     2581 atggtgtagc taattatgta gataaaaaat ttatttatgc ctaccctgtc atttgtgttg
     2641 tattcagaat acttttacgt ccatttatat ggagatggtt acaaatacat gctttttata
     2701 gtgatactat tgcagattat ttaacgaatt ttatgtgttt tgtagcgttg tctatagaaa
     2761 tcttctctat actttttatt tacaatattg ccagacatgt tacaataatt atttttatag
     2821 atatacttgt atttattgct ttactgataa taggtatgta taaattattt aaaaaataca
     2881 attaattttt aaaaattaag taataagtga tagtgtaaaa agtagccact gcaaattatg
     2941 gattgtagtg gctattttat tatttgtata tgaaattatt ttgtttttct aataagttac
     3001 gatatattat ggtttatttc actgataaaa ggtttaggta ttttaaagta aaaagttgta
     3061 tattctaata aaatttaaac attttgattc tttaaaatta gagaaaatac attgctttta
     3121 ttaaaaaatt aaatattaag aatttaattt ctgcatctta tttagaaaag ataagaaatc
     3181 aactttatat attttttttg gccttcctct ttgattatcg agttttgtat ttacaatagt
     3241 tgcaattcct gctttatata aaatactcaa tattctatta gcacttctaa gagttatgtt
     3301 catatagtct gctaatattt ctgctgacac attatttgaa tttctatcat taatcataca
     3361 catgatttta gatacatttg tattagatag aggtataaaa gaagcaactt tattggctat
     3421 tgagctatct tcaatattat tagaattttt atttgaatat aaaggaccta ttgtatcatt
     3481 agtatcattt actataaagg tacagtttcc attcaaagaa gcagatttac cattagcttg
     3541 acgagcattt tgctctgcat gaatatttgt atttccaatg ccccaaccta tatttaagct
     3601 atatttaatt tcttttaaat cttctgaaat aaaaaaatct gtatagttat tggtcatatt
     3661 gtttaacatt tctttagtag tgtgtatctc tattttcata tcattttttt tgacctgaac
     3721 tccatagaat ccatgtcttg atagagaatt taatatagtg ttatatattt gattttgtat
     3781 ctcatcattt ttaaatatca aatcttcatt tatgtactct gaactattta ctgtaactat
     3841 acaagttgca gatttatttt tgtctaagtt ttgtaatgtt agttcattca ccaaagattt
     3901 aaatgtattc aggattgatt ctgtagatgg aaccatgtaa atacaatcat atccaagctt
     3961 attaagttca caactaatga tgtaatttct agtaaaagaa aggttaatct tattatctcg
     4021 caataagtct aaatgtattt ccaataattt atcatttaat tttttaaagt catatacagg
     4081 ttgagggata ttaaaatctt taattgtata tggacattta ttagagtcta ttaatgaata
     4141 tatatcataa taattgttgt cctctcttaa aaaatccata aatgactttg taaagtcaat
     4201 atctttttgg ctatttaata atttgaataa gtatttataa aaatctcttt catcaagctt
     4261 taaaaaattt aatggaactt ttatgtcttt tatatttatt ataagttcac tatatggaac
     4321 tataccacta catacaaatc catcaaaatt atctacttgc tcatagtata tattaggtaa
     4381 attattaaaa ggattctccc ctttgagctc aataaatgta atttcacaag tatcatttaa
     4441 ttctttaaga gcttgtttaa acttatctga cataaaataa gatgttgtca aaaaaccaat
     4501 tttatatttc ataaaacctc ctttaaaatt tcgagagtat tgtaactaat caatcaattt
     4561 ataacctcat tttataatag tagataaaac gcgtcaattt attttacaaa aggataattg
     4621 aaagaaaaat cagaaaatat aaaaaagatt gaaaaatatg ttgactggca agaataattg
     4681 tgctattttg taattaagga ctagtctttt aagatttcct aaaataaact taggttttag
     4741 gaaagaaata cattttattt gaaaataaag gagaagatta tgaatacata tttaaatgaa
     4801 atttctaagt ttgttgatga gaatagagaa gaaatagttt cattatggaa agagattgtt
     4861 aatatagaaa gctatacaca ttgtaaagaa agcgtaaata aattggctaa gagattaaag
     4921 tttgagtttg aaaaagaagg tttagattgc gagttagtag atgttggaga taatggtagt
     4981 acattgatag gtacacttgg ttctaatata gataaaaagc caataatttt ttcaggtcac
     5041 atggatactg tctttgaaac aggtacattt ggagagaatc catttaaaat aatagaagga
     5101 aaagcatatg gcccaggtgt tttagatatg aagggtggta taataatttc cttatatgtt
     5161 attaaagcat taaataaaat aggatacaaa gaaagaccaa taaaaatagt attttctgga
     5221 gatgaagaga taggacataa ggattcaaca ggtgcagatg ttatattgag ggaagcaaaa
     5281 ggtggtcttt gtgcttttaa tatggaaact ggattagtag ataatagttt gtgtgtaggt
     5341 agaaaaggaa gaataggatg taatatacat gtaaaaggtg tagaaacaca cgcaggtaat
     5401 gattttgaag gtggaagaaa tgctatagaa gaaatggcta ataagatact acgcatacaa
     5461 aaattgacta atttagatgt tggaactaca gtaagtgtaa gtgtgattaa gggtggaaga
     5521 gtggaaaatt ctattccaga agattgtagt ataaaagtag accttagatt tgaaaaagta
     5581 gaagaaatgg agaatgttaa agaacaagtt agagagatat gtaaagaaac ttacatagaa
     5641 ggaacttcta cagaagttaa ttttataagt gaaatgatgc catttgaaac tacagaagat
     5701 gttatgagat ttcatacatt tgtaaatgaa gtgtcaaaag aaaatggatt tggagaatta
     5761 aatgctaaga gacttggagg tagttcagat gcaagttatt taactattgc aaacgttcct
     5821 acaatctgtt catttggtgt aagaggagaa tggaaccaca catcaagaga atatgcagta
     5881 gtagattcta tgtttgagag ggtgaaatta atttctacag ttattttaaa cttagataaa
     5941 tttgaggtat aaagggggat tttatatgga tgtaacagct ggagataata aggtaacatg
     6001 gaaagggtat ttatctttat ttgtattgat agtattattt tcaggagtgt ttaaagatag
     6061 tcaaggcttc cttaaagcat tagattttgc taatttgaca ggttcatttg gtactattgc
     6121 agatggtgta gactttatgg gtaaaggagg aacgggagca aaagatggtt tcttatttgc
     6181 tctgactttg attccgactg tctgttttgc tgtaggtctt atagatgtag ttgaatctat
     6241 gggaggaatg aaagcggcat caaagatatt taatccattg cttagacctc tgcttggtat
     6301 acctggaata gcaggaatag catttgtatc ttcatttact agttcagatg tagcctcaat
     6361 aatgacgaaa gaattatttg aaagtgggga aataacagat gatgaaagaa ctatatttgt
     6421 agcatatcaa tatgctggtt cagctgttat actgaacaca ataaatactc aagctccact
     6481 tttaccaata gcattattgg cattaggtcc aataatcctt atagagatat tctgtaaaat
     6541 acttggtgct aatctagtta gattaatcat aggtgtaaga aataaaaaga ataaggctaa
     6601 ggaggctgtg tagtatgagt caacaaaata ctggaaatgc aattgtaaat agtaaaccta
     6661 agaaaactgc tgttgagata tttatggggg gagcaaaaaa aggtttttac ataggtgttg
     6721 aacagatatt accagccatg ataatgggtt atgtaatagt gcagttttta caattaactg
     6781 gtttagttga tattttagga aatatttttg gaccagtaat gggtattttt ggactacctg
     6841 gggaatctgt tgtagttttg atagcagcat tctttgctaa ggcagcagga gcagctacag
     6901 ctgcaaactt atttgcacaa ggtctaatca ctgcaccaca agcaaccata ttaattatgc
     6961 catgtatgtt aatgggtact ctagttggtc attatgccag aatagtgctt gtagcaaatg
     7021 ccagtactaa gtatagaatg ttactgttgg cagttcctat atttgatgca atagtgggga
     7081 tgttaatagt aagagtttta ctcactgtta tgggattaat gtaaataaca taaaattata
     7141 acctaactat aaatgatata taagaattaa tatatacaag gattaatgaa tagtaattga
     7201 tatatagtaa taaactaaga tagtaattaa tgtgtatatt aattaaaaaa aagagagtat
     7261 ctcaaagtga actttttagt tcatgagata ctctttttgt atgaaatcaa gtatattttc
     7321 ataatttcaa caatgaaaga gaggcttatc tctattttga gacagcctct tcttttaaat
     7381 ttatatttct agaatttatg ttttttattt atccaacatt aacattagtt attagaaata
     7441 attttaagaa aaatatactt tcatgttaat atcagaataa atctgttttt aaaggatgat
     7501 ttgttttgaa ttttcacgaa tatctacttc tataactttt tctattgtat ctcctctaaa
     7561 acctaataaa ttacttgtca agtttgcagt ggaacaagaa tgattaggta taattcttat
     7621 tctatcacca atttttatat cagtttcacc atttacttta atttttccaa cttcttctga
     7681 aagtgaatca atagtaagtt ctggatgtcc acatacccta ccataaccaa caatactact
     7741 atttccatgt gcacctttat caagtccaag gcacttactt ccacaatcaa ttaaatagta
     7801 tccactgcta ttttttgaga ttacagtgca cacaacagta agagcacaca tatcttcagt
     7861 agcacaacct aaacctatct gtatagcatc atagtataca tagtttccag gtcttacagc
     7921 agttatagtt ttagaagcag cttcagattc aactgttgga gtactaccag aagcgacaat
     7981 ttctatatta aatccattat cagttagagc tttgtatgct ttctccatag aagaatcttc
     8041 ttgaacacaa acatctggaa caccttctgg agaactacaa ccatacactt gacctggatg
     8101 cgaacttata ccaacaatct ttatgttttc tagcttagaa atttcagtag caagttcaac
     8161 cactttctct ggttcaaccc caaaacggtg taagccacaa tctacaataa taagtagctt
     8221 gcactctatt tttgatttag aaaaagcttc actataagcc ttagcacatt ctatattatc
     8281 tgtagcacag attaaattta tttttgatgc catagttacg attctattta tatttacttc
     8341 tcccatgtaa ggataagcca acataatatc ttcaaaacct gcttttatca gagcttcacc
     8401 ttcatcaagt gttccaacta gaaaaccact agcacctgca tttttttgaa gtctcgctat
     8461 gtaagtactt ttatgtgtct taagcatagg ccaaagttgt ttattattat ttgtacaaat
     8521 ttgttgaatt ttatctatat tcttttctaa ctggtctaaa tctagcaaaa aatttggagt
     8581 agcgacttct gaaattttca tgttatttac cacctttcaa caaaagtaaa ttaactataa
     8641 gtattataat tattatatta gtaatatcgg ctttataata ttaatagctt ataattatat
     8701 tatagaacta gcgctaacag agagcaatat ttatttgtaa taattagaat tttcaataag
     8761 ttagtgtagt tttaaattat ttatataata tatttttaat tttatgcaaa gtttatttaa
     8821 attagtttga ggatttatta atagggtatt attgaatgag taaaaagtga tgtttacata
     8881 gaataatcac attataaatt aatttagaac ctaaaaaatt aattgagcaa attggaaaag
     8941 agaaggataa aaaagatatt tggatagttg gtggaggcga tatagtaaaa ttatttatga
     9001 aagataactt aattgatgat tattatatat atatattgcc aataatattg gggagaggga
     9061 tttcactatt taagtctggt atagataaga ctaatttgaa ttttaagaaa gctagtaata
     9121 ttggagaact tgtaaaattg gaatacagta aaaaacaaaa ataaattaca tgccaatttg
     9181 ttacaatgtt atgaaatgaa gcatttaaaa gacttttggc tatatgaatt gacattactt
     9241 gtcaaatatg ttaacatata agcgacttgt cggagggtgt attaaaaaga atatgctgga
     9301 gaagagagtg cgttaactct tttctccttt ttttataaaa ttaagcattt ataaatttag
     9361 tattattaca tgattacact atctcaaaaa ttaagaaagg agaaaaatat gaaattaatt
     9421 ttaagttatt taaagaatta taagttatta atagtattaa acatactggc tatatttagt
     9481 tttgcattag tcgagttggg gattccaact ataatagcta aaataattga caatggtatt
     9541 gcaaatcaga atatagctta cataaaacaa atgggaatag tgattgttat tatttctatt
     9601 ataggggttg taggtagtat attgttgggt tattgttctg ctaaggtatc aacaagtgta
     9661 accagagata taagaaatga catatttgag aaatcacaag aattttcaca tactgaatac
     9721 aataaatttg gaatttcttc aatgattaca agaacaacga atgatgcatt tcaaattcaa
     9781 caatttgtta atatactact aaggactgcc ttattaactc cagttatgtt cattataagt
     9841 ataataatga caattagaac aagtgtggaa ctttctatag tattggctat atcagtacct
     9901 tttataatta ttggagtagc aataatagca aaagtatctc aggcaatatc ttcaaaacaa
     9961 caaaaaggac tagataaatt aaaccttata tcgagagaaa atttaactgg aataagagta
    10021 attagagctt ttggaaatga tgattatgaa acagagcgat ttgaaaaaac aaatacatat
    10081 tatgctaatg tttctaagaa actatttaag cttatgtcaa ttactcaacc agcattcttt
    10141 ttactattaa atatagctgt tttggctgta ttttggattt caagtgagaa gataaatatt
    10201 ggagagcttc aagttggaca gttggttgca tttttggaat atcttttcca tgccatgttt
    10261 tcaattatgc ttttttcaat ggtatttatt atgtatccaa gagcagaggt atctgcaaat
    10321 agaataaaag aacttttaaa tgaagaacca ttgataaaaa atcctgaaaa tggaattaag
    10381 gatactgaaa ataagggtat catagaattt gataatgtaa cttttaccta tccagatgga
    10441 gaagcttctg tacttaagga tatttcattt actgcaaaaa caggtgaaac agttgctttt
    10501 ataggaagta ctggtagtgg gaaaagtact ctaattaact tgatacctag attttatgat
    10561 gtaacagaag gtagtattaa gataaatgga gtagatataa gagaatatga tttaaaagct
    10621 ttaagaaaaa agataggatt catacctcaa aaatcactgc tttttactgg tagcatagca
    10681 aataatatta gatttgggaa acataaggca ggagagtcag aattagaata ttccgcaaaa
    10741 gttgcacaag catatgagtt tatatcaaaa aaacctagaa agtttgatga attgattagt
    10801 gaaggtggag caaatgtatc aggaggtcaa aaacaaagac tttctattgc aagggctatt
    10861 ataagaagac ctgaaatata catttttgat gatagttttt ctgcacttga ctttaaaact
    10921 gatgcaatac ttcgtgctaa attgaagaag gaaacaaaag atgcaattgt attgattgtt
    10981 gcccagagaa ttagttctat tattgatgct gataagatta tagtactcaa tgaaggacaa
    11041 gttgtaggaa tgggaactca taaagaatta ttaaaaaact gtgaaatata ctatgaaatt
    11101 gcaacatctc agttaaaaaa ggaggaatta gaataatgaa aaagtctaaa ggttttaaga
    11161 aaacaattgg tagacttctt ccatttgtaa agaaatataa attttccttt attatagcta
    11221 ttatatgtat aatttcagca gctactatga atgcacttgc acctaaaact gaaggattga
    11281 taataacaca attgactaaa gatgtaatta gtatcgcaaa aggagtacca ggggcttccg
    11341 taaattttga ttatgtaaca aaaatattgg tgattttagc atgtatatat tttgccaatg
    11401 caatttttac gtatacttca agttttctac ttacgaatgc tattcaaaac acaatgagag
    11461 atttaagaaa tgaagtcgaa aataaaataa gaagattgcc tattagttat tttgatagta
    11521 atagttttgg agatgtatta agtcgtatat caaatgatgt agatacaata tcaaatgcat
    11581 tgcaacagag ttttatgcaa attgtaaact caatcttagt cataattttg gcactttcaa
    11641 tgatgtttac aattaatata tatatggctc ttatagctct ttttataatt ccaattagtt
    11701 attttgtatc taaatttgta gtaaaaaaat ctcaaagtag attttcattg caacaaaatg
    11761 ctcttggaaa attaaatgga aaagtacaag aaatgtatac tggatttaat gaaataaaac
    11821 tatatggtaa agaagaagat tctataaaag aatttaaaaa agtaaatcaa gaactttgtg
    11881 aaaatggatt taaggcacaa tttatatcaa gtatgatgaa tcctatggtt tcattagtaa
    11941 cttactttgg aattgcagct gtggcagttg tcggttctat atatgcagta agtggaggaa
    12001 taacagtagg aaatttacaa gcatttgtgc gttatatatg gcagataaat cagccacttt
    12061 cacaaatgac tcagttatct acagtaatac aatcttcttt tgcagctata gaacgtgtat
    12121 ttgaaatttt agacgaagaa gaggaaatac ctgatgttga aaatccagtt aagatagaaa
    12181 atgtaaaagg aaatgttaca tttgagcatg ttaattttgg atatggagaa aattcaactt
    12241 taatagaaga tttaaatgca gaagtaaaaa gtggtcagat ggtagctatt gttggaccta
    12301 ctggagcagg taaaacaaca ttaataaact tattgatgag gttttatgat gtaaagaaag
    12361 gtgctattaa aattgatgga gtagatataa gagatatgaa acgtaaagat ttgcgttcaa
    12421 tgtttggtat ggtgcttcaa gatacatggt tatttaatgg aaccattttt gaaaatatag
    12481 aatatggtag atttggggct actaaagaag aaattataca agcagcaaaa gtagcaaatg
    12541 ttcatcactt tataaccact cttccagatg gatacaatat gtttttaaat gaagaagcat
    12601 ctaatatatc tctaggggaa aaacagcttt taaccatagc tagagctttt atatcagacc
    12661 cttcaatatt gatacttgat gaagctacga gttctgttga tactaggctg gaacttatgc
    12721 ttcaaaaagc aatgagaaat cttatgaatg gtagaactag ttttgttata gcacatagac
    12781 tttctacaat tcgcaatgct gacttgattt tagttatgaa caatggaagt attatagagc
    12841 aggggaatca tgatgaatta atggaaaagg gtggatttta tgagaagctt tataatagcc
    12901 aatttgcaga caaagaatca gaatagataa cataaacttt tgtataaaat tttgtatcaa
    12961 attcaaatat taatcatata attaaaaaga gtatcagtaa gttgtttttt tatagattta
    13021 ctgatactct ttgattttaa gactaaaggt atttatgatt ggtacttata attcacaact
    13081 agtcttgtta catctgataa ccagaattac agtacttttc ttttttacct ttttgttctc
    13141 taacatataa caattcgctt aacttatcaa tgtaataacc atcatcactt atgcgaatta
    13201 aactagtcag ataatatcca tctttgcttc taggaatctt tttatttatt gctaaatcaa
    13261 tagctgtctt tgctgcttca aaatgcttta aatgtgtatg accttcttca aaactctttc
    13321 tagtattata aactacataa ccttttttaa cttgaaaaac tataaattct ttcttttcat
    13381 atattttatt aaacattcaa tcagcccttc tcaagatagt tttatagcat aacattagaa
    13441 gcttctaaaa aattttacaa agaacataaa tattttataa attataaatc taaaactata
    13501 atttataaag ataactttct gattatttat atgataattc atatagatta aaagtaaatt
    13561 atctttaggt taaacaaaag taaaataaaa gactagttat aatatttata tgaaaaagta
    13621 caaaatatag tatatgaata tagaaaattt aaattattta tttacagttt ttatttccat
    13681 aagtatattt accttcagat accttgtcat catggaaata tattctttca caacttttat
    13741 ctccccatag aatgtttttt gttattttat gggacgtatc ccctgtaaat acttcagcta
    13801 cagtagtatc tgtaacatca cagtaaagag aagcgaattt tttaaaccaa ggattttcct
    13861 caaatcttaa aagccaagaa gctccatagg ggcaatgtaa ctcttcaaat tcaggaaccc
    13921 atcctagaaa tggaacatca gttgtgctaa taatattgtc tgcaattaat tcatctccat
    13981 tttcatgagc tctctctctc atagcaatag ctctttcttc agacctttca aataaaactt
    14041 ttcttaatat ttcgctagtt ttatcttcac caaactcttc aaataacact ttggaaaaat
    14101 aaaaatataa atcaccaaaa tgtttgcatg ccatacgaac ttcttcaata gcataatctt
    14161 tgtcgatagt ttcattagtt acaacatctt cgttcaaaaa aatcactcct tagaataagt
    14221 attagttaca atataattat agaatatttt ttataaatta ggaaattaat ttgagataga
    14281 tatattttta ataaatgaag gtataatgct aaaataactt attgttacaa aaatcttcta
    14341 aattaatgta tttaggagtt tccatatgaa tgagagtagt tctgtaaata tcttcaatac
    14401 atccatttgc aatattatta aaaatatctg tgtgctcttt taatgttgtt tcaagccttc
    14461 cttcatgtga caatgaatcg agagctaatt tttttattct gtacatgtaa ttgttaaaca
    14521 ttttgtcaaa ttcagtattt tgagcatatg aaactatagt tgtgtgaaat aatgtatcgt
    14581 attctgcaaa tgagtttaaa tttttatcac ctaataaaat atctttttgt ttatctaaaa
    14641 gatgttttaa tttacttata gtttctactg ctctagcttc tttgtattga cttgtaatta
    14701 aaaaagtaca aaacccttct attgcagagc gtatttggaa tatctctaca atttcatttg
    14761 ctgtaatttg atggattcta aatcctttgc ttgggattat atctataaat ccatcttgac
    14821 taagtctttg aagagcatct cttataggtg ttctagagat acctatttca ctagcaagtt
    14881 ttgtttcaga atatatttca ttttctatca tttctccaga tagtatcaat tcttttaagt
    14941 agtcatatgc ttgttcttgt aatcttttaa atgtagccat acttaataat acccccctaa
    15001 acaatataaa aatacaaaaa cttatataat tttaagcata caacatagtg aaaataaggt
    15061 caatcttttg caatttaaat tgcagataag attaaaaaat ataaaaaatt attgaagtgt
    15121 gaagcatgat taacattaaa ggcagattaa ttaaaactaa ttgaaattag ttaaaataca
    15181 aaactgtaaa agtagtatat tatatttgaa aacaatagta tgtattaaaa cttatatagg
    15241 atgactaaat ataataaatg actttaaaaa ttactattta ttgttaaaat aaatactttt
    15301 atattttata ttgaccggta tactggtatg tagtatactc taaaacataa aaaacgtttt
    15361 caaaaatgtg aagatatgga gggttataaa atgaaattgg gatttattgg attaggaata
    15421 atgggaaagc caatggcaaa aaatttatta aaagatggat gtaatctttt ggtgtatgac
    15481 attaataaat ctgctgtgga tgaacttata tcatgtggag caaaatacgc aagtgtttta
    15541 gaaatgggtc aagaatgtga tattgtattt acaatccttc caaatggaac tattgtgcag
    15601 gatatattgt ttgggatgga tggattggca aaaactttaa aagaaggaag tatagtagtc
    15661 gatatgagtt cagttactcc tacagaatcc atattgtgtg caaataaatt aaaagatatg
    15721 ggattagaat ttatagactc accagtaagt ggaggggagc caaaagctat tgatggtaca
    15781 cttgctttta tggctggtgg taaggaagaa atttataaga aagtagagcc attttttaat
    15841 ataatgggaa gctcatctat tttaattgga gataatggta gtggaagtgt tacaaaactt
    15901 acaaaccaag taattgttaa tttgacgata gcagcagttt ctgaggcatt tgtacttgca
    15961 gcaaaagcag gtgcagaccc agaaaaagta tataaggcaa taagaggagg cctagctggt
    16021 agtacaatat tggatgcgaa gataccaatg attatgaatc gtgattttaa accaggtgga
    16081 aaaatatcta ttaatttgaa agatattaag aatgtaatgc aaacagcaca caatttagat
    16141 gtaccgctac ctatgacaag tcaattatta gaaattatgc agacattaaa agtacatgga
    16201 catttggaag atgaccatag tggaatagca cagtattttg aaaagttggc aggtgttgaa
    16261 ataaaaaaac acaataatta atatttaaat atatcaatat aattaaatac atattattag
    16321 gattagatta tataaataca gcgaggttaa gttatcatag taaaagaaaa ataaaattga
    16381 taaataataa agtagaatga taaaggggta aagattatga ttacaggata tggacttttg
    16441 atagcatttg ttatatctat aggaatttta cttgtatcga ttattaaatt taaagtaaat
    16501 ccgtttttgg cattactgat aacatctata attacaggct ttatggttag aatgcctata
    16561 aatgaaatat caaccacaat atcgactgga tttggaaata cattaggtag tataggtatt
    16621 gtaattggac ttggaataat atttggaaat attctttcag aatcaagagc aacagaatct
    16681 attgcaaagg ggttgttagc taagactgga gaaaaaaaca gtgctttagc agtaactaca
    16741 gcaggttttt taatttcaat tccagttttt atggatgcag cttttgttat aatgatgcct
    16801 ataattaagt atgtttcgag agtaactaag aaatctctaa tggtttttgt atgtgcctta
    16861 ggagttggga ctatagtagg tcatgcactt gtaataccta caccaggacc tttggcagtt
    16921 gcagcaaatg taaatgcaaa tgtaggtagt tttatacttt attctataat tgttgctttt
    16981 ccagcagctc ttataggtgg atggatttat ggaaaacgtt ttgaaaaata tccagcttat
    17041 gctattgatg agaatgatag agaaaaaaat ttagagcagg gaaaagatag cataaaaatt
    17101 gaagatgatg attcttctaa agtaccaggt tttggaattt ctatgttttc tttattgttc
    17161 ccaatacttc ttatacttat atcaaatgta tttagtatgt tcttagaaaa aggttctaca
    17221 atgagtggag tacttgcatt tataggagat aaaaacatag caatacttct tggaatattg
    17281 gtggcaatag gatttttaaa gaaatacata aataaaccta tgggagatgt agttattgaa
    17341 gctgctgatt cagcagggct aatattattg ataacagggt caggtggtgc gtttggcagt
    17401 gttataaatg caagtggtat aggtaacttc ttagttgata ctatgtctgg tcttagtata
    17461 tcagttgtag tgcttggatt cttattaagt gccttgctta gaatttctca aggttctgct
    17521 actgtagcct tagtaacaac atcatcaatt ttaggaccta caattttggc aactggaatg
    17581 tcaccagttt tagtagggct tgctatatgt gctggaggag ttggtttctc ccttccaaat
    17641 gattctggat tctgggtact ttcaagattt agtggattat cagttaaaga tactttaaac
    17701 tcttggacaa ttggaggaac cattgctgga gttgtagcat ttataatggt tttattgctt
    17761 agtgttataa atggaattat accacttcca ggtctataaa taacaaacta ttgtttatat
    17821 tatataaaat tacatttaga ctatacaaat tacataaagg ggtgttttaa atgagagaag
    17881 taaaaccaat tagtatagat atacttaata cttttaaaca agtagatgag gatagattaa
    17941 atgaacttct tgcagatgag cttaagtatt tagatagaaa aattgttgta ttagatgatg
    18001 acccaacagg tgtacaaaca gttcatgata tttctgttta tactgattgg gataaggata
    18061 gtatggaaca aggttttaat gagaaaaata gtatgttttt tatacttact aattcaaggg
    18121 gatttactgt tgctcaaact acaaaagcac ataaagaaat ttcaaagaat attgtggatg
    18181 tatcaaagaa agttaataag gattttataa tcataagtcg tagtgattct actatgagag
    18241 gacattatcc tcttgaaact aatttattaa aaagcgaagt agagagatta tcggggaagt
    18301 tatttcaggg tgaaataata atgccatttt ttaaagaagg tggtaggttt actatagata
    18361 atgtacatta tgttaaagaa ggagaaatac ttgtaccagc aggaatgact gaatttgcaa
    18421 aagataaatc ttttggatat aaatcatctg atataggtga gtggtgtgaa gaaaagacaa
    18481 aaggaaagta caaatctagt gatatgatat atatttcgtc aaaagaactt agagagctaa
    18541 atattgaagc tataacagaa aaacttaaaa aagctgaagg atttaataaa attattgtaa
    18601 atgcaaccga ttatgtagat gtaaaggtat ttacaattgc gtttatacga gcagtaaata
    18661 gtggtaagga atttatgttt agaagcgctg ctgccataac aaaagtactt ggcggagtga
    18721 gtgacaagga actacttact aaggatgagt tagtatcaaa aggtaatact aatggaggaa
    18781 ttatacttgt tggttcacat gtgaataaga ctacccaaca gttagaagaa cttaaaaatt
    18841 gtaaatatcc gatagaattt atagaattta atcaacattt agttcttcaa gaaaatggtc
    18901 taaaaaatga ggtaaaaaga gttataaaag ttgtagagga aaaaataagc agtggtaaaa
    18961 ctgttgctgt ttatactaga cgagagcgtt ttgacttgga tacgaatgat aaagataaac
    19021 agcttatgat ttctgtagaa atatcagatg cagttaccag tataattggt atgcttaatg
    19081 taagaccaaa ttttattata gctaagggtg gaattacttc tagtgatgtt ggtactaaag
    19141 cactaagagt aaaaaaagct acagtaatgg gacagataaa acctggtata ccagtatgga
    19201 tgacaggcaa agaaagcaaa tttcctaata tgccatatat aattttccct ggaaatgtgg
    19261 gagagattag cacacttaga gaatcagttg agatattgat ggggtagtat attagtgtat
    19321 tgaagtttaa acgcttaaat ttactgtgtt aaaagtaaaa taaactaaaa ttataaatta
    19381 agattaaaaa taatagtata ttaattaggg tttagagtat tttacaaaaa atattctaaa
    19441 ccttaatctt atgtattagt atttttatta tttaacttat gtgtttattt tattatttat
    19501 cttttttaat tgatttaata ttttctttga ttttatttaa aatatctgta tgcttattag
    19561 agcttatgtt ggggaatgct ttgattatac gtctatgaag taatttgtgg ttagacagca
    19621 aagtattaag tttatttttt agctcatcaa tacgattttt agcatagcga taattttctt
    19681 tttctttaag tatttcaaac ctatcttcca aagtatccag tctattttca actaaagatt
    19741 caagtttgtt ttctatagtt tcttgtgtaa taagtttgta gtattctttc ttatctttta
    19801 attccttaat tataagattg atttgtttta acttaccatt taattgtaaa attgtatgga
    19861 ctgtgataac taaatctaaa acaaaataaa tttctataga tatagcaaat ataaatagta
    19921 catttttaga gatagagtta ataaaatcaa ccacaatagg atgtactata agcattacaa
    19981 tcaatgacaa tacaccaaac ataagtgagt tttccagaca aactcttcca tgaatattaa
    20041 atttatgttt agaataatcc caccaagtca tattaaatat tttttctaaa ataaagctcg
    20101 tacaatactc cattatgcta gttataatta tactaaacaa aaataacaaa ggaatattgt
    20161 gtacaaaagg agtcaaggta gcaactattg ctaatgctcc aaatccatat atagggcaaa
    20221 atggaccatt taaaaaacct ctatttacaa attgtttttg taaaactgaa caatatgtag
    20281 tttcacaaca ccatcctata aagctataaa ttacaaaata aagaaattga atataaaatg
    20341 tactcataag cacctcattt ctagttagta taacactata tactcgatat tttattttta
    20401 tgattaattt actaaattca atttacatat tgattatacc aatttataga aatttatcaa
    20461 atttaattac agttagaaca ttataaacat ggttataata atgaaatttt atttaaaata
    20521 aataagggtg tcaagtataa tcaaactaat agttgtagat actctacact atgttagttt
    20581 aattacattt gacaccctta tatttatact atgactattt acattcgaaa ctagagcaac
    20641 atgtttcaat ataaaggttt actctatcag tttcaggagc aaccaatata ttatcaagtg
    20701 tacataagct accatctttg ttgtatttac aattttttac agtacaaact aattcctcac
    20761 aatttctatt attaacgttg ctagataaat tactataaat agattcatct aaaaatgaac
    20821 tacaacaagt atgtctgtca ttgtgagctt tttcaccatt tataaaaatt ttatctgcat
    20881 aacatatatg gctgttgtta tgtgaacagt taattacatt acagtttatt ctttccaaag
    20941 ttattacctc ctaaacgatt atttattata aattatgtcc atataattac aagaatataa
    21001 ccattaagag aaaactacct tttagaaaat atctattctt agagtaattt gaaatataaa
    21061 tatattttct tctaaaattt acctaaattg tataaaaact tacagcttgg agaaaataat
    21121 agaaaaagga ggcgtaatca atgtttgaaa agaagaaaaa agaattattt ccttggaaaa
    21181 tatctgcctt aatactggtt ggagtatttg tattaagtag cggagtttat atagggacaa
    21241 gattaaaaca caatggagtt caaactggtg aagttgtaag tactagtgcg aatatgaatg
    21301 aaaagtcaaa agaagctttt ggattaaata aaaattgtga agtttggctt caaagtgaag
    21361 gcgaaaatga aactaactat aagagaacac ttacaatggc aggaatagtt ccagagtcat
    21421 tattagataa gacgaagtca gaaatagtat catattttaa aaaagaatat ccaactaaaa
    21481 aaataaagag tatggataaa aatgaaatca tattggtaga atctaaatat agtgataata
    21541 aagatgtaca agcaatggat gttactataa aaggtaaata tactattgaa aatgataatg
    21601 gaaatatagc actatataaa tatgatgata gcggaagtaa aacattagta gaaaaaacaa
    21661 aaataagagt agattcactt cctaaaacag ttcaagatga aatcaaaaaa ggtgtagtta
    21721 tggatactga agaagaggcc tattcaagat tagaagactt tgcaagttaa tagtaaatta
    21781 atattatttc aagttaaata ctaagtaagt taataataaa taatttaata ttaaatagat
    21841 tagtattaaa attgtagtca catttaattc ctataaaaat acaataattt atttgtaata
    21901 aatataaaag ttatattgag gtaaaatctt gctttgatat aactttttta tttgatttta
    21961 tattttacat agagcatttt atgatttgtt aatattattt atgatataat gatttagaac
    22021 ataagttttt atgagaaata cattttggta tttcttttat ttttaggggg aattttaaaa
    22081 aatgataata ttggggattg acccaggtat agccatagtt ggatatggta taattgaata
    22141 caaaaatagc aagtttaaag taatcgatta tggagcagtt acaacacatg cccatatgaa
    22201 tatatcgaga agattggaac ttgtgtataa aggaattgat acaatagtaa agaattacaa
    22261 tatagatgaa gttggaatgg aagaattatt ctttaacaag aatgtaaaaa cagctataac
    22321 agttgcacaa gctagaggtg ttactatgct tgcatgtgct cataatggga agcctgtata
    22381 tgaatacact ccacttcaag taaaacaagg tgtagttgga tatgggagag cagataaggc
    22441 acaagttcaa cagatggtaa cttcattttt aagtctaaaa aaagttccaa aaccagatga
    22501 tgttgcagat gctttagctg tggctatttg ccatgctcat tcaaacaaac ttgaaaaaac
    22561 tctaaagaat ataggtggta agtatgtata gttatataaa aggtacagta gaagaaattt
    22621 atatagacag tatagttgtt gaaaataatg ggataggata taaaataaat gtatcatcca
    22681 atacaataat gaatttacaa gtgggagaag ctacaaaaat atatactaaa ttaattgtaa
    22741 gagaagatga tatgagtctt tgtggttttg taagtaggga agaacttaag atgtttgaat
    22801 tactaacatc tgtatctaaa ataggaccta aagtggcgct ttcaatacta tcatttgcat
    22861 caccagcaca gttaggagca tatattttaa gtgaagacat tggaaaatta tccaaagctc
    22921 ctggtgttgg taaaaagaca gcagaaagaa tcgtcttaga gcttaaggat aaggtagaca
    22981 aaaataacat agagtttgaa ccaacgttat tatcacaaaa acctaccttg atatcacaag
    23041 atgagtctgt agatgcactt gtagctttag gctatacttt gtcggaatca aaagaggcag
    23101 ttcaaaaatg taaaaaagat ggtatgaata cagaagctat tataaagaaa gcacttacat
    23161 atataatgag taaatccttg aaatagtatt tggattttat aatatttgga ttttaaaaca
    23221 atatttaagg agtttatgta aatttgaaaa tatataaagt agattttcaa aagtatgaga
    23281 ggaggggtat aaatgcaagg ttttgaagat gaaaatagga taataacttc tacaatgaag
    23341 atggaagata tagatataga aaacagttta agaccaaaaa ctttagaaga ttatttgggt
    23401 caagaaaaat ccaaagaaca acttagtata tttatagaag cagctaaatc gagaaatgag
    23461 cagttagacc atgtgctttt atatggacca ccaggtcttg gaaaaactac actagcaagt
    23521 ataatagcca atgagatggg ggtaaattta agaattacgt caggacctgc aatagaaaga
    23581 gctggagatt tggcagccat acttacgaat ttaaatgaaa atgatgtttt atttatagat
    23641 gagattcata gaatcaatag aagtgttgaa gaagttttat atccagcaat ggaggatttt
    23701 tgcctagata taataatagg gaaagggcca tctgctagaa gcataagact tgaccttcct
    23761 aaatttacac ttattggagc tactacaaga gctggtatgc ttacaaatcc acttagagat
    23821 agatttggtg ttatttgtaa attggattat tatacagttg atgaactttc aaaaatagta
    23881 cttaggtctt caagcatctt agatgcagag atacagtcta atggagcatt agaacttgca
    23941 aaacgttcaa gagggactcc aaggatagca aatagattac taaaaagagt tagagatttt
    24001 gcacaagtca gagctgatgg aaaaataact gataaggtgg caaaagatgc tctggaactt
    24061 ttaggagtcg atagtttggg tctcgatttt gtagatgaga agcttcttat gactataata
    24121 gaaaaattta gaggaggacc agtgggttta gatacattgg cagcttctat cggagaagat
    24181 agaaatacaa tagaagatgt atatgagcca tatttgcttc aattaggatt tataaataga
    24241 ggtcctagag gaagagtagc catgccatta gcatatgaac acttaaaaat tccatatcct
    24301 aatgaaaaat aaaaatagat tatacgagta tttataaaat aaaagagtca ttaaattaaa
    24361 atatttctaa aatggctaaa aattcaaaca tatccacaaa atggtaatta tatctgtaga
    24421 tactgtggat aaacacgata aaaattgtgt gtattgtatc tatatattgt ggataagaca
    24481 tgtatatcgt ggaaaaatat ctatatattg tggatatggt gtctgttaaa aatagcaatt
    24541 tcttgataga ttatggtaat attgtggaaa ataagttgtt tttttgatat tattgtagct
    24601 ggagaaattt taagagagga agtttaacat tgaaaacaag tgattttaaa tttgacttac
    24661 cacaggaact tatagctcaa gttcctatag aagatagagc tagttcaaga ttgatggtat
    24721 tagataaaga gacaggtaat attgaacata aagtatttag agatataata gaatatttaa
    24781 atccaggaga ttgcttagta ttaaataata ctagagttat accagctaga ttaataggag
    24841 agaagcttga aactggaggt aaaatagagt ttttattatt aaaaagaaca gaggaagata
    24901 cttggcaggc tttagtaaaa ccaggaaaaa gagcaaaagt agggactaaa ttttcttttg
    24961 gaaatggaaa gctaattgga gaagttgtag atttatcaga tgaaggttca agaattataa
    25021 aattccatta tgatggaatt tttgaagaaa tattggatga acttggaaat atgccactac
    25081 ctccatatat cactgctaga ctagatgaaa aagaaagata ccagactgtt tattcaaagc
    25141 ataatggttc agcagcagca cctacagcag gattacattt tacagaagaa ttgttaaata
    25201 aaataaaaga aaaaggtgta gatatagcct ttgtaactct acatgttggc ttaggaactt
    25261 ttagaccagt aaaagtagag gatgttttaa atcacaagat gcattcagaa tattatatgg
    25321 ttagccaaga agcagctgac aaaataaata gagcaaagga aaatggaaaa aatgtaatat
    25381 gtgttggtac tactagttgt agaactatag aatcagcttg taatgaggat ggaaagatga
    25441 aagaaactag tggatggaca gaaatattta tatatccagg ttataaattt aaagttttag
    25501 ataaacttat tactaatttt cacttaccag agtctacact aataatgtta gtaagtgcta
    25561 tttgtggaaa ggataatgtt ttaaatgcat ataatgaggc agtaaaagaa agatatagat
    25621 tcttcagttt tggagatgct atgattataa agtagggagg taatggatgc atagtttaca
    25681 aaaacaaaaa aagaataacc ctatattaaa gattgctttt atatttgaac gtgttttagc
    25741 agtagtagtt ctaatagcag tatttttagg aactatagat gttctaagac ttatgtgggg
    25801 agcatatata gttgattttc aaaatccagt acaatacagt cagcttaatg attttttagc
    25861 acaaatatta cttttagtaa taggtgttga acttgtagta atgctatctc ttcatatacc
    25921 aggagcattt attgaggcat tactttacgc aattgctaga aaaatgttgc tactgcctaa
    25981 aaatgaaggt atgatagatg ttttaatagg agttatagct atagcaggac tatttgctat
    26041 aaaaaagttt ttagtaacaa aagacaagag tgctcttaat ataatgagta ttcatgatga
    26101 agaagcaatt aaggaacaac agaacaaaga agacgaagat aaatctattt agacaataaa
    26161 ttatatgaat agcttattac aaatatatct taagaaataa tgagaaataa atatgattaa
    26221 ggagaacttt atgtacgcag taagatacga attgataaag acttgtaagc agagtggtgc
    26281 aaggttagga agattacaca ctccacatgg gattattgaa acaccaatat tcatgccagt
    26341 tggaactcaa gcaactgtaa aatctatgac accagaagaa ttaaaagaga ttggttcaca
    26401 aataatactt agtaacacat accatttata tatgagacca ggtcatgaac ttataaaaag
    26461 agctggtggt cttcataagt ttatgaattg ggataaacca atacttacag atagtggagg
    26521 gttccaggta tttagtctag ggcctttaag aaagataaaa gaagaaggtg tagaatttag
    26581 gtcacactta gatggttcaa aacattttct aactccagaa aaagctatgg aaatacaaaa
    26641 tgcattgggt tcagacataa tgatggcatt tgatgagtgt gcaccatacc catcagatag
    26701 agaatatgta aaaaattctt tagaaagaac tacaagatgg ttaaaaagat gtaaagatgc
    26761 ccataataat accgacaagc aagctttatt tggtataata caaggtggga tgtataaaga
    26821 cttaagggaa caatcagcaa aagaaataac taatattgat ttaccaggat atgctattgg
    26881 tggacttagt gttggagaac caaaaccact tatgtatgac gtgttagagc atacaactcc
    26941 acttatgcca aaggataaac caagatacct aatgggagtt ggtagtccag atgacttagt
    27001 agaaggtgtt ataagaggag tagacatgtt tgattgtgtt ctacctactc gtatcgctag
    27061 aaatggaact gcaatgacta gccaaggtaa agtagtagtt agaaatgcaa cttatgctga
    27121 agactttact ccacttgacc ctgaatgtga ttgttatgct tgtaaaaact attcaagagc
    27181 ttatataaga caccttataa aagcaaatga aatactgggt gcaagattaa taactactca
    27241 taatcttcac tttttattaa atttaatgaa acaaataaga caagcgataa tggaagatag
    27301 attacttgat tttagaaatg aattttttgc aaaatatgga tatgaaatat aggttttgca
    27361 ctattaatta taatcatttt aatatataat attcaatagt actcaaagat agagaaagta
    27421 aattgttata taaacattta ttatgattta caaaagagta taatttacaa aggagtgaaa
    27481 aattaatgcc acagcaacaa ataattatga gtataggtct atgggtagta gtaatcgcaa
    27541 tcttttattt cctaatgatt agacctcaaa agaaaaaaga caaacaatta aaagaaatga
    27601 gaagtagcct aagtgtagga gacaaggtca ttacaatagg tggaatagta gctaatgtag
    27661 ctaaagttga agatgatgtt gtaatattag aattaggacc aaatagaact aaagttccat
    27721 ttgaaaaatg ggctatagga actgttaaat ctaaaaaaga agaaatagaa gaagactaaa
    27781 ttagtaattt aaaagccttt tctcgaatat atattaattt gagaggaggt ttttttatgg
    27841 gaaaatctaa ttatattttt aaaggtctag gatatgcata tataataact ttggctgttt
    27901 tattagtgta taacttattt ttgacattta cagatattgg tggagataat ataactatgg
    27961 tctcatcatt tataactact atttctgcag ctataggagg gttttacact tcaaaacaca
    28021 tgaaagaaaa aggacttatg tatgggcttt tagtaggact tttgtacata gtatgtattt
    28081 ttttgactgt gtttttagca caagaaaagt ttgtatttga agtagggatg atttataagt
    28141 tattgttaat atcagcagca ggaggcattg gaggcgtatt gggtgtcaac tttaaataga
    28201 gtttatatta aagtagtttt atgatataat caataggaaa caattagaaa tggaggcgtt
    28261 gaaaatggaa aataaacatg taaaaacttt atctcaagct actttaaagc aaagtgctgc
    28321 aaaaggtgga tgtggagagt gtcaaacttc ttgtcaatca gcttgtaaaa cttcttgtac
    28381 agttgcaaat caagagtgtg aaagatagaa aattattgat ttagttgttt gtaagagggt
    28441 atctcaaagt gattctttta tcattatgag gtatcctctt ttattttcaa ttattatata
    28501 aaaatatatt ctatttatgt aaaattaaat ttgatttctt atcgtttaat tatgcaaaaa
    28561 tatctaatca tataagagca ttcattttaa agtagctact cttatatttt attttttatc
    28621 aattacatgt attagaataa tttttaattt taaactctaa tttatgatga tttttataat
    28681 atgttatata ttttaatatt tggagataga tattaattta gtgatatata tttaagtaat
    28741 aagagtaaag caaaaattag tgcatagtag tatataaaaa tggagtttga atacttttgt
    28801 tagtatatga ttgtttagta gaaaatcact ataaaatagg gatttctatc tttgagcata
    28861 attatagaga gctaagcagg gggaaggggt tcaagaagat attattagtg tttgtgttag
    28921 taaattatgt aaacacataa aatatattga aaaataatat tatatataat attatttatg
    28981 tgtgggaaat acgaatataa aaaataaaaa ataatattcg gaggacataa atatgttaag
    29041 caacaaaaag agaagtatgg caatagtaat ggcaggtgct acagttatga gtgcagcagc
    29101 accaatattc gcagacaata ctgtgacaga aaatgttgat aaaaattaca cagtaagtgc
    29161 gaaagattct gctaaactaa tcgaagaggt tagaaaagca ttagaagtta agtttgaaga
    29221 tacaaaagca ggagcaaatg taaatgatag agtttacgat ataaaagtag acaatgtaaa
    29281 cttaactaat gcaactcaat tacaaaataa gataaactct ttaacagaag ggcaaagttt
    29341 aaaagttact attcaagata aaggacatca agtgttaggt ggaaaagtag ttgactataa
    29401 aattgaaaac tacaaaactg ctcaagaaat agtagatgca gttaatgctt acaatgcaac
    29461 tttagcagaa gattctgata acaagttaac agcaactata aaatctacaa atacagttga
    29521 agtaaaaaga gcaaaagatt cagcaaatgt tattacatta aatgtaggag accaacattt
    29581 agatttctca aaagtgataa catcagaaga aggtactttt gaaggttatg aaaagagtta
    29641 tagtgacata gattcaaaag aattacatac gataacagtt aaaaacgcag atttacaaga
    29701 catatcagca gaagaattat ttgatggaat tagattaact acattaggaa gagaaatagt
    29761 taataaagtt aaaaatggat atgctttaac atttgaaaat gaagcaatcc ttactcaaga
    29821 acaagaagat agtgatgata aagataaacc agaaaaatca agctttgata tagttttaag
    29881 caaggcaaat gaaaagccag aaacaataag tgtttcaagt aaaaaccata agttagttag
    29941 agacttacac aaagtattaa ctgatgtaaa agatggaaaa gaattaaaag tagaagtttt
    30001 atctggtgat tcaagattta caacagcagt agaagttagt aaagagagat tcaaagatgg
    30061 agaagcagaa gctataattt tagttggaga agatgctata gttgatggat tagcatcagc
    30121 accacttgcc tctcaaaaaa atgcaccaat attattatct aaaaaagatt cactaccatc
    30181 agaaatagaa gctgaaatat taagagtact tggaagtaat ctatcttcta agaaaatata
    30241 tatagtaggt ggagaatcta aagtatcaaa agaaactgaa gaaaaacttt ctaaattagg
    30301 tgtaagtaaa gttgagagag tttctggaga agatagattt gaaacttctt tagaaatagc
    30361 aaaacaatta aaagatacat ttaagactgc ttttgtagta ggtggaaatg gagaagctga
    30421 tgctatgagt atatcagcta gagctgctca atttggtgct ccaataatag ttacaggtaa
    30481 cgaattagat gcaaatgctg aaaaattatt aaaaggaaaa gaattagaaa tagtgggtgg
    30541 agaaaattct gtatcaaaag aagttgaaga caaattagtt gatatagatt taaataataa
    30601 agttgaaaga ttagctggag aaaatagaaa agatactaat gctaaagtaa tcaataaata
    30661 ctatgcaggt gcaactaaag catatgtagc aaaagatggt tatgtaggtg gaaatggaca
    30721 attagttgat gcacttacag cagcaccact tgcagctagt tcaaaggctc caatagtatt
    30781 aactacagaa gaactttcta aatcacaaga agaagtagtt gagttaagac ttaagaatgc
    30841 tactaagtta gtacaaatag gtgaaggaat agctaaaaac gctattgaaa aaatagcaga
    30901 aaaaataaac ttatttacta agaactaata agatatagga attaatatta aaagtttatt
    30961 tagataatat atgattatac aatgcgttaa tatgtaagac atagtgtaga tattataaaa
    31021 tatacaaata acaagataaa ataataaaaa taaggtgtct catttagaat tagattgaga
    31081 taccttattt tattttttga ttataatgtt gagaaattca tattgcaaaa attattataa
    31141 aaagatactt cttataatga ttaaacatct
//
LOCUS       DAEMIK010000031        30838 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEMIK010000031 DAEMIK010000000
VERSION     DAEMIK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.031"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..476)
                     /gene="pilM"
                     /locus_tag="KPK87_001214"
     CDS             complement(<1..476)
                     /gene="pilM"
                     /locus_tag="KPK87_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG2537381.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLN"
     gene            complement(529..888)
                     /locus_tag="KPK87_001215"
     CDS             complement(529..888)
                     /locus_tag="KPK87_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG2537382.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(942..2027)
                     /locus_tag="KPK87_001216"
     CDS             complement(942..2027)
                     /locus_tag="KPK87_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG2537383.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAELFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVAITRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(2198..3577)
                     /locus_tag="KPK87_001217"
     CDS             complement(2198..3577)
                     /locus_tag="KPK87_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG2537384.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3570..4250)
                     /locus_tag="KPK87_001218"
     CDS             complement(3570..4250)
                     /locus_tag="KPK87_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2537385.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4641..5720)
                     /locus_tag="KPK87_001219"
     CDS             complement(4641..5720)
                     /locus_tag="KPK87_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2537386.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      6008..6123
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6298..7668
                     /locus_tag="KPK87_001220"
     CDS             6298..7668
                     /locus_tag="KPK87_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2537387.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWISASVCFVGLILTVFFMRK
                     TDKSIS"
     gene            complement(7934..8545)
                     /locus_tag="KPK87_001221"
     CDS             complement(7934..8545)
                     /locus_tag="KPK87_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG2537388.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8535..10898)
                     /gene="lon"
                     /locus_tag="KPK87_001222"
     CDS             complement(8535..10898)
                     /gene="lon"
                     /locus_tag="KPK87_001222"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG2537389.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            11078..11590
                     /locus_tag="KPK87_001223"
     CDS             11078..11590
                     /locus_tag="KPK87_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2537390.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11578..12105
                     /locus_tag="KPK87_001224"
     CDS             11578..12105
                     /locus_tag="KPK87_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2537391.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(12176..13426)
                     /gene="clpX"
                     /locus_tag="KPK87_001225"
     CDS             complement(12176..13426)
                     /gene="clpX"
                     /locus_tag="KPK87_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG2537392.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13445..14029)
                     /gene="clpP"
                     /locus_tag="KPK87_001226"
     CDS             complement(13445..14029)
                     /gene="clpP"
                     /locus_tag="KPK87_001226"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG2537393.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(14179..15465)
                     /locus_tag="KPK87_001227"
     CDS             complement(14179..15465)
                     /locus_tag="KPK87_001227"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG2537394.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEVIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLLGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(15659..16129)
                     /locus_tag="KPK87_001228"
     CDS             complement(15659..16129)
                     /locus_tag="KPK87_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2537395.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(16129..17478)
                     /gene="rph"
                     /locus_tag="KPK87_001229"
     CDS             complement(16129..17478)
                     /gene="rph"
                     /locus_tag="KPK87_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG2537396.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNVHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17638..19662)
                     /gene="ligA"
                     /locus_tag="KPK87_001230"
     CDS             complement(17638..19662)
                     /gene="ligA"
                     /locus_tag="KPK87_001230"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG2537397.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKEMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(19847..20785)
                     /locus_tag="KPK87_001231"
     CDS             complement(19847..20785)
                     /locus_tag="KPK87_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG2537398.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(20887..21309)
                     /locus_tag="KPK87_001232"
     CDS             complement(20887..21309)
                     /locus_tag="KPK87_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG2537399.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21497..22780
                     /locus_tag="KPK87_001233"
     CDS             21497..22780
                     /locus_tag="KPK87_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG2537400.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(22858..23373)
                     /locus_tag="KPK87_001234"
     CDS             complement(22858..23373)
                     /locus_tag="KPK87_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2537401.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23373..24749)
                     /locus_tag="KPK87_001235"
     CDS             complement(23373..24749)
                     /locus_tag="KPK87_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG2537402.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(24774..25325)
                     /locus_tag="KPK87_001236"
     CDS             complement(24774..25325)
                     /locus_tag="KPK87_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2537403.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25383..25598)
                     /locus_tag="KPK87_001237"
     CDS             complement(25383..25598)
                     /locus_tag="KPK87_001237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537404.1"
                     /translation="MQIHRELLKSENEYMCTKKEKQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(25567..26400)
                     /gene="fdhD"
                     /locus_tag="KPK87_001238"
     CDS             complement(25567..26400)
                     /gene="fdhD"
                     /locus_tag="KPK87_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG2537405.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVRKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26449..28593)
                     /locus_tag="KPK87_001239"
     CDS             complement(26449..28593)
                     /locus_tag="KPK87_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(28177..28179),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HBG2537406.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(28844..29902)
                     /locus_tag="KPK87_001240"
     CDS             complement(28844..29902)
                     /locus_tag="KPK87_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2537407.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(30113..30772)
                     /gene="cprR"
                     /locus_tag="KPK87_001241"
     CDS             complement(30113..30772)
                     /gene="cprR"
                     /locus_tag="KPK87_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG2537408.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTIREHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT         9308 a         5567 c         3325 g        12638 t
ORIGIN      
        1 atatttagat tttctgaaat gttcttaatt aattgaataa tactctttgg gaaaagaatt
       61 acttgtatat tcaatttatc atttttagaa tttataactt tatattttat tgaatagtta
      121 tttatgttta ttggaatgta ttgtgttatt tcaaattcaa tcatgcttaa tatgtctctt
      181 tttttcttag tatttagaac ttcaacattt ctaatgacta tatcctgaat ttgaatgtta
      241 aagtataaat ttttatataa tatttttgat ttaccaatct cctcttttac aaatctaact
      301 acatcttcta tatatttaat cttctcaatc gaattactat cattttttat atcaaagtta
      361 atctctttta taattgattt atctataaaa acttttttat gactttttct taaaaataca
      421 agccttatat aatcatcatt tatttctaag tttagaattt ttttaattat agccatctcc
      481 ttcttaaata aaaataattt taaaacttac agcaatcaat ataaaatatt attttggata
      541 aaattgctta gaatttatat ttacgcttat atctgttccg ctatcattta tgacaagttc
      601 gaatttacct gatgtactct gaggaactgg aacagtattt aggtatccct tttccttcaa
      661 tgtctctaca cttagagagt ctattgtctt ttcatcatca ttaattgcca tgctagctgc
      721 ggttactata ttagcagcat tcgtatagtc tgcatttaat ttggcacttt cttgaacctt
      781 actatactta actaatgcta tactagatat aacgcctaaa atagttacta ctacaatcat
      841 ttcaacaagt gtaaatcctt ttcgtttttt atttatcaaa tttatcaatt aaatattctc
      901 tccttttcaa aagtttcatc taataaaatt tatttttctc tctaaaaatt agtaattgca
      961 tcaaacatag gaacaaccat tgctaaaata aaagtaccaa taattaatcc cataaataca
     1021 attagcatag gttctataaa tttcattatt tgctctattt tgttatttaa ttcattttca
     1081 taaaatttat ttattacatt tagactttta tctagtttcc cactttcttc tcctgtattt
     1141 gccattgaaa taaataattt tggaaaaaca tttgctaaat tcaaagattt acctatgcta
     1201 tttccttttt ttatataatt attggaaatc tttaaccttt cataaataaa ttcgttgtct
     1261 ataacactag ctgacatgtc tatagcttcc acaatttgta ttccactgct aattaaaacg
     1321 gacaatgccc ttgaaaatct tgttatagct actaactgat taattttttt tataaaaggt
     1381 attttgaatt ttaacgaatt aatcaatttt tttatattat catttgttat aaagaaataa
     1441 atgcttccta ttgaaaacaa tataaaacta aatattatat aagctaagtt atttgtaacc
     1501 actgtagata ttcccattaa aactctagtt attaaaggtg gttcaattcc attgttggta
     1561 aaaaccacct gaaaattagg tattacaaat acgaatataa acaacattga taagatagat
     1621 aatattataa gtattattgg atatattgaa atagttatta ttttcatttt taatttgtat
     1681 tctttatcat aataattaga taaatcactc attattatat ctaagtttcc actagtttct
     1741 cctgctttta tcatacttat aaagaattta gaaaatatac ctgttatttg aaataattct
     1801 gctattgagt tacctttttg aatatgattt gatactatac taagcaaatt ctttactttt
     1861 ttacttgact gactctcaat gatttcaaat atttttgtaa tttcacatcc agattccagc
     1921 aatatgccta tctctttgca aattactcta atttctttat cactgattct atttaatact
     1981 aaataaaact tttctaaatt ataaaatata tttttagata atctcatgta ctataatacc
     2041 ttccaaatta tattaattac aattaaataa acttaatatt ttctaattta atgcatttaa
     2101 ttttatttca cttaaatata atttatactc aaacatctta attaaaatag attaaataaa
     2161 gacaactgac caaacgaagt ttaactatac aattcagtta aatcatttca tttactacta
     2221 aacactcttc taatgtcgtt attttttcat ttattagtct tttacaacta tcttcaaatg
     2281 ttatcattcc attattttgt gccacatctt taatagtact agaatcctcc atatttctta
     2341 tacatgtctt tatatcatca ttaatttcta agatttcata aattgctgtt cttccaaagt
     2401 atcctttatc attacatttt tcacacccta cagctacctt agtatttaca tctccactaa
     2461 aattatcttt tatcataatc tcatggctac aatgattgca gacttttcta accaactttt
     2521 gagatataac acctataaga gaggcattta aaagatatgg tggtatttgc atttctagta
     2581 atctagctat ggaagatact gcatcattag tatgtaaagt acttataact aaatgacctg
     2641 ttatagctgc tcttactgct atcttagctg tttctgcatc tcttatttct ccgaccatta
     2701 ttatatctgg gtcttgtctt aatatcgctc taagacctac atcaaagctc aaccctactt
     2761 ttgaatttac ttgaatctga cttatgccat ccatcttata ctctacagga ttttctatag
     2821 tcataatatt tttatttatt ccttttaaat catttaataa ggaatatact gttgtcgtct
     2881 ttccactacc tgtaggacct gtcactaata atattcctgc tcttttattt attatacgat
     2941 ttatagattt tatagcttcc tctgaaaaac caagttctaa tttgtctttt aaaaaagtat
     3001 ctctattaag tatcctcaaa actatttttt caccatagac agttggtatt gatgaaactc
     3061 taatgtcaat taaatttcca cctaacttta tatccaccct accatcctga ggaagccttt
     3121 tttctgttat atccattgat gcctttaatt ttataaccgt agacaatgat ggatagacat
     3181 caactaaaaa ctttgatact tcctttagtt ctccatctac cctcattctc actattaaat
     3241 gttctttaaa tggttctata tgtatatcac ttgcgttttc ttttatagct ttttctaaaa
     3301 gattttcaaa taattttttt gcataatcct catcaagttc aaagctatta taatattttt
     3361 caatcttttc acttataagt tcttttttct ccttcttcaa tataatttct ttacctgtag
     3421 ctaatctcaa gtcctgtaga gcataaatat cctcatcttc tatttctaca actaaattat
     3481 catcttgaaa ctcaactgga aatacattat atttttttgc aagtttttct ggtattaaca
     3541 ttggcaaact gctttcctct aatataatct taatcaccct ttatatactt taatttatta
     3601 ggtatattca tttttttcat agagatattt tcttcagttt ttatgcttat tttcattgga
     3661 taattggttt gtatgctatc taaatattta ctacctttat ctacataatt agatagtttc
     3721 tccttatctc caatgcactt tatttcatat ggttgtgcta caggaactcc atttatattt
     3781 atatggcttc ctgcaagtac aacttctgaa tattgattaa ttctttgacc atttatttct
     3841 atgtattttc ctccgtttac ttttaattca tttataactt ttacaagaat cttattatag
     3901 tctatactat tagctatatt ccctgttttt tcatctatag catctatact taatattatc
     3961 ccactaccct ttacatctga atatgataga ctatctctaa gtgtttctat ttctgatatt
     4021 tcttcagaat ttttttgatt ttgtttcatt gaattaattt ctttatctaa cttgtctttc
     4081 tctttggtta attcttttat agattttctt gtcattttta tttcttttct tattgacttg
     4141 tctttgcttg tataaactgt ttcttcattg ctataatctc taaatatggt tccaacaagt
     4201 atacctaaaa tagtacttgc tagtattata aacacatatt gttttttcat actgtattgc
     4261 tccttacaaa tttattttta tatgataatt ttctttaatt aaatattaaa catttacata
     4321 tgcttattat atcaaagtta tatattaaaa ttcatcttta gtatataata tcatacattg
     4381 aactcctatc actaatttac caatatcaag tatgcatttt tagatgttct taaaattata
     4441 taaactttca ataaacattg ttgtgatatt ataaaaaaca taaattagga aaggaaataa
     4501 taattatgaa aaacaaataa tgactttgga gattattaat atcatttttt agcaaaaaaa
     4561 agtaaaatgg catagcctca aagctacacc aatttactaa aaattatatt attattaaca
     4621 ttaattttaa acatatacgt ttataagttt tcctcatatt ttaaaattct tttaattaac
     4681 ttatctctta atttgcctac ttcatcaaat ctcatattag gactatagta actttctata
     4741 atatcatgtt tagacttggc tctcttaaga tttagtactg cccaacaaat taaatcatct
     4801 aaaacttttt catcaaattt aatatcttct tcatatttaa gtagtttttc tctatttaaa
     4861 aactgattta aatcaattgt atcttcttct ttaaaagtcg tacttatagt tatgcctata
     4921 tctaaatcag ttaatagtac agtttcaatt ttctctggaa taagtggata atgataaact
     4981 tctacattta aacctttttc aatagctttc ttatatactt tgtttaataa tgtagttttc
     5041 ccagttccta tttctccatc taaataatag actttcttta catcttttaa aatgttttct
     5101 gtataatcta aacaccctac aggagttata gctgaaccaa ataaatgctt ttctcttttt
     5161 agatttcctg tgcttttaac tccatcaaat aaccttttta taaattcttc tgtgaccaaa
     5221 tttacttttc cataatccat acaatcagaa tatttttctt ctatgtctct aaagattggt
     5281 tctgctgatt ttaaaaactt aaatgctctt ttaaatgaat tactaatatc tttatcataa
     5341 actgatattt cttgtttatt cttttctaat tcttctgtat tccagtaatc acctaaatta
     5401 attatctcat ccaaggctcc aggtagcttt gggtccatag tatgtggggc agttgcatcc
     5461 ataagtacca cgcctaattt ctttataact aaaccatcca acgaacttgg gtcagaagga
     5521 caatgatata aatcaatatc atatcctcta tgcaaaaact ctttataaat ttttttcata
     5581 agtgaggatt ttcctactcc aggtccaccc tttaaacaaa aaactctttt tacattttca
     5641 ggaattatat agtcaaaaaa agaatatgag ccttgtgatg tatttccacc cgggaatgcc
     5701 ttctttacct ttccttccat attagcgcct cctcataatt tacaatatct cttaattaag
     5761 atattcatat tataagaatt ttattacagc tttctgattt tgacaaattt aaaacttgcc
     5821 atctaatact aataacaggt taattttatc taattattat agattctcat actgtgcctt
     5881 attctatcta taaatacaat ttaagtgtcc atattgaaat atttgtattg taatacagct
     5941 ggatattact taaatccaat tgtttccatt ataattttat gttaaaataa tattacaaaa
     6001 tacatctgtt tttcttcata aacgggtgaa attccctatc ggcggtaaaa gcccgcgagc
     6061 cttatggcat aatttggtca tattccaaag ccgacagtaa aatctggatg gtagaagaaa
     6121 atagtatatg agtaccttta tgtaatttta catgagtaat ctatacaaat ccttcaacta
     6181 ccgtatttat tcatgaaatt agacacattc aaggtaccta atatacaggt gctttttttg
     6241 ttgtttattt tacaattata tcgtacttat aaaatctatt aagattggag tgttatcatg
     6301 aaacaaaaat ggatagtatt gattatcatc tgtattggtg tatttatgtc tactcttgat
     6361 ggaagtatac taaatatcgc aaatcctaca attgctgctg actttaaaat taacatgtca
     6421 caaattcagt gggttgtaac agcttatatg cttgttgtaa cagctactat gttatttttc
     6481 ggaaaacttg gcgataaagt aggttcaaat cgtctataca cattaggatt ttttatattt
     6541 acaataggtt cttttctgtg tagtatgtca aacaatttat caacattaat ttcttcaaga
     6601 atctttcagg cagttggagc aagtatttta atggctactg ggcttggtat agtttccaat
     6661 gcatttccag caaatgaaaa aggaaaagct ataggaatca ctggagctgt agttggtata
     6721 ggaaatatga gtggtcctgt aattggaggt ataattttag aacattttgg atggccttct
     6781 atttttatta tcaatatacc tattggtatt atagcagtct ttcttggcat taaatttctg
     6841 cctaaacctg ttttggatga acaaaacaag agctttgata ttccaggtct tctacttttc
     6901 gcatcttgca ctacgcttat attacttgca atgaatgaga aaggtaatac aagattgtat
     6961 ttaggtataa cagcacttat aatcttctta ttgttggcat taagagaagt aaaatttgaa
     7021 caaagcttta ttgacctacc tctatttaaa aatagaaact ttactgtagg caatatcatt
     7081 ggagtagctt gttattttcc tcaaatggca gtatcttttc tacttccatt ttatttagaa
     7141 caattaaaaa atttatctcc tatgatggct ggttatgtaa tgactgtaca tccacttatt
     7201 atggtactta tagcacctat tgcaggttca ctttctgata aacacggtgc aaaaaacatt
     7261 ctaacagcat ctttttcatt tatgaccatc tcactagttg gaatggcact tttaaaagca
     7321 gattctccat tgtatctttt aattgtatgt ttagttattt ttggacttgg gctaggagca
     7381 tttagttcac caaacaatag tagtatttta gcagatgttc ctcctcaaaa acaggggtac
     7441 ggaggcagtt ttcttgcaac tattcgtaat ttatcatttg ctcttggaac agcatttttt
     7501 agtagttttt ttgcacaaag tcttacatac aatcaaaagt ttaaaagtca cacatcagcc
     7561 tatgttatag ctagcaatca gtcttattgg atttcagcat cagtatgttt tgtagggctt
     7621 atactaactg ttttctttat gaggaaaaca gataaatcta tttcttaaat gctatttatt
     7681 actgactaca taaatcaata attttttagt ttaactattt tatactaatt ttcccatacc
     7741 ttactaaaat ttattagtat ttagcaaaat tactgatatt tattgtttat aaacaataag
     7801 gatgccaaaa aaaagttcat taaaactatt ttaaggcatc ctatatttta ctttaatatt
     7861 aatataatta aaactaacta ttattacatt tattattcaa ataaatctta tgtcaatatt
     7921 taacatttta aatttaatct actgttattt catatccatg ttgattgtat tgatttacaa
     7981 tctcttccca taattcttca acaccagtct tttttagtga agaaacagga attacttttt
     8041 cactactttc catttgcaat ttttttctta ttatacttat gtgtttttga tattgtcctc
     8101 tagatatttt atctgcctta gttgcaataa ctacacagtt atatccaaag tgctttatcc
     8161 attcatacat aagcttatca tctgctgtag gctcatgtct aatatctaca agtaggaata
     8221 tagaacataa ctcttgtcta ctttctagat atcgttccat tatacctccc cacttttctt
     8281 tttcagactt agctatcttt gcatatccat agcctggaag gtctacaaaa taaaattcat
     8341 tatttattag ataaaaattt atagttctag tttttcctgg tgtttgactg gttctagcaa
     8401 agtttcttct gttaagcaat gtatttatta ttgatgattt tccaacattt gacctaccag
     8461 ccaatgctat ctctgggatt ccttctgctg gatactgtga tttattcaca gcactcatag
     8521 ttatctctga acttctaatt ttcattattt tcaccactct ttaataaagc ttgttctaat
     8581 acttcatcca tatgttctac aagcacaaac tccatttttt cttttacatt ttctggtatt
     8641 tcatctaaat cagcttcaca ttctttagga attaatactt ttgttatacc agctctatga
     8701 gctgctaata acttttcttt aactcctcct actgctaaga ctctacctct taaagttatc
     8761 tctcctgtca ttgctatatt tccaggcaca ggtcttttag taagtgctga gattactgca
     8821 agagccatag ttataccagc tgatggtcca tcttttggaa cagcaccttc tggaatgtgt
     8881 atatgaatat cattcgtctt ataaaattca gggtctatat caaacttgtc cactattgac
     8941 cttatataag agatacctgt cttcgcagat tccttcataa catctcctaa ttttccagtt
     9001 aatacaattt cgcctttacc tttaagtacg tttacttcta cttcaagtgt aactccacca
     9061 acttctgtcc acgcaagacc atttactaat cctatttgag ggttaacttc tgcaagttga
     9121 tacttaaaca tgtcttttcc aagatatgtc tctaggtcag acttatttat aacaacctct
     9181 tcaagagttg ggtcttcaac gtattttttg gcaacctttc tacaaatctt accaatagtt
     9241 ctttctagtg ttcttacacc agcttctctt gtataatggt tgattatact tcttaaagtt
     9301 tcatcatcta tttttataaa gttttctttt aaagcatgtt ctttaatttg ttttggaagt
     9361 aggtattttt ttgctatatt taatttttct tcttctatat atccagatac ttctattact
     9421 tccattctat ctaataatgg tcttggtata ttacttagac tatttgctgt tgtaacaaat
     9481 agtatttttg ataaatcaaa aggtatttct aagtaatggt ccacaaaatc cttattttgc
     9541 tctgggtcta atacttctag cattgctgaa gctgggtcac ctttataatc tgctgccatc
     9601 ttatctatct catcaaataa gaatacagga ttttttgttt gggcttcttt taccccatta
     9661 attattcttc ctggaattga acctacataa gttcttctat gacctcttat ttctgcttca
     9721 tctctaactc cacccaaaga tattcttaca aattttctat ttaatgctct ggcgattgat
     9781 tttactatag atgtctttcc tgttcctgga ggaccaacta gacatattat aggcccttta
     9841 agtgacttag caagagttct tatagctaga tactctagta ttctctcctt aactttttct
     9901 aatccataat ggtcttcatc taatatatct tttgccttag ttatatctaa tttatctttt
     9961 gtctctttat tccatggcaa tgagaatata gtatctaaat agtttctact tacagataca
    10021 tctggagaca tagaagatat ttttgaaaat ttatctatct ctttttctat tttctcttta
    10081 gttgtcttag gtgcttttat ttttttaagc ttttctctat actcatctgc ttcagagttt
    10141 atatcttctt cttcacctaa ttctttctgt atagctttta attgttctct aagataatat
    10201 tctttttgaa ccttattcat ctgttttttt actcttaaag ttattttctt ttctattttt
    10261 aatatatcta tttcctctaa taatatagaa taaataagtt caagtctttt tctaatatca
    10321 aattcttcta atatttcttg tttttggctt gattttagat atatgtttgc agctatcgta
    10381 tctataaatc tatctacatc ttctatatca gctaaagata ttaatatttc tggtgatacc
    10441 ctatttccaa tatttatata ctcttcaaag gcatcaaata catttcttac aaatgcttca
    10501 atttcaactt ctgtttcact atccaaatta tcactatcaa atactatttc ttctattact
    10561 gctctgaaat acccatcttc ttgttctatt ttctttactc tcccccttga tacaccttca
    10621 accaaaacac gaactgtatc tcctggtaat tttatcattt gcttaacttt acagattgtt
    10681 cccacatgat aaaaatcctc ttctcctggc tcatctacct ctgcttcttt ctgagaagtt
    10741 aaaaatataa gttcttcatc catcatagct tggtctaaag ctttaagaga tatctctcta
    10801 cctatatcaa aatttaatat catatatgga aatatagcta atcctcttag aggaattaat
    10861 ggtaattcat gatctatttt agtataattt tgttccatca acattactcc ttttctagtc
    10921 taattgcttt gcttttaata ttatatattt gaacaagtta attttcaaca ttaatgataa
    10981 attcttagaa tactatcata ctatattatc tttttattat gatataatat taatattagt
    11041 atacttagat gttatttatc ttttggaggt ttctattatg ttgaaattgt ttttaacctt
    11101 tttaaaaatt ggtgcgttta catttggtgg tggatacgct atggttcctc ttatacaaga
    11161 tgaacttgta aacaaacaaa aacttttaga gcaggaagaa tttattgatt atttatccat
    11221 agctcaaagt tatcctggag tactggcagt taatatatct gtattattag gatataaact
    11281 ttatggtata ccaggagttc ttatatgtac cttaggctct gctttaccat cattttgtat
    11341 agtcttagtc ttatcgtatc tatactttaa aaattcatca tctaaaattt tagatggatt
    11401 ttttaaagga gttgcgcctg ttgtaatagc tcttatattg tattctttta cgagcatgtt
    11461 caagaaacta ccaaagtcta aaactaacat actattatta attatagcag ttttatctgt
    11521 tggtgtattc aatataagcc ctatttattt aataattgga ggaggtgtat atagtctatg
    11581 tcagaaataa tactactctt cttaatattc cttaaaatag gggcatttag ttttggtggc
    11641 ggttatgcca tgctaccatt tatccagcaa gaatttatat ctaaatatca ctttataact
    11701 aatcaagagt ttttggattt acttgctata tcacaatcta caccaggtcc tgtagcaata
    11761 aatagtgcta catttgtagg atttaagact gctggcttct ttggttctct agctgcaact
    11821 attggtgtta caatttttgc attaataggt ctaactatta tatcaaagtt attttctaaa
    11881 tttaaaaata ataaaaaggt agaaaactta ttgacagttc ttagaccaat tactctagga
    11941 ttcatattat cagcagcttt ttcatctgcc aaagaagtta attgggactt atatgggata
    12001 attgcattta tagcatcatt ttatctttta tataaaggaa aaattggttc cattgcaatt
    12061 gtattcgtct ttggtattct aggtatatta ctcacatttt tataataatg atacctattt
    12121 tatatttagc atattaacat aaaaaaagga gcttttaaag ctccttttca tcatcttatg
    12181 cactttcctc ttgatcctta agtactatga taggatttac tgagtcttcg ttcacagatt
    12241 tctctgttac tataaccttc tttatattat ctctagatgg tacttcaaac atagtttcca
    12301 tcattacgct ttcaactata cttctaagtc ctctagctcc tgtatttctt tctatggcct
    12361 ttttagcgat tgcacgaaga gcaccttctt caaattctag ctcaacatca tctaactcca
    12421 ataacttttt atactgtttt actagagcat ttttaggttc ctgtaatatc tgcattaatg
    12481 catcttcatc caacaactct aaagtagcta aaactggtat tcttcctata aactctggta
    12541 ttataccata ttttaacaaa tcctctggaa gtactttttc atatagcttc cctaaatcta
    12601 attctttttt actttctatt ttagctccaa aacctagtgt tttatctcca ccacgttttt
    12661 gaattatctt ctctaatcca tcaaaagctc cacctaatat aaataataca ttagtagtat
    12721 ctatctttaa aaattcttga tgaggatgtt ttcttcctcc ttgtggtgga acatttgcaa
    12781 cagtaccttc taatattttt agaagtgctt gttgaacacc ttcaccacta acatctctag
    12841 ttatagatgg gttttcagat tttcttgcaa tcttatctat ctcatctata tatattatac
    12901 ctctttcagc tttttctata tcaaaatcag cagcttgaat aagttttaaa agtatatttt
    12961 caacgtcttc tccaacatat ccagcttcag tcaatgatgt agcatctgcc attgcaaaag
    13021 gtacatttag agttcttgct agagtttgag caagtaatgt ctttccagaa cctgtaggtc
    13081 ctaataaaag tatattactc ttttgaattt ctatatcctt actactagat tttttactgt
    13141 atattctctt ataatgatta taaaccgcaa ctgataaagc ttttttagct ttttcttgac
    13201 ctattacata atcatttaga atctccatca tttcctttgg ttttggtaaa gatgtagtat
    13261 cctcatctat agtatcttct atctcttctt gaataatttc gtcacaaagt tcaacacact
    13321 cgtcacaaat atatacattt ggacctgcaa ttaatcttct tacttgatct tgatttttac
    13381 cgcagaatga acactttaat tgtcgttttt cttcatattt agacatatta acacctctct
    13441 ctatctacgg tctctttgta aatacttcat ctattaaacc atattctttg gcttctagtg
    13501 cactcatgaa gttatcacgt tcagtatcca ttttaatttt ttctaatggc tgaccagttc
    13561 tttcagataa tatctcattt aaagtttctt ttatttttaa aattctcttt gcatgaattt
    13621 ctatatctgt tgcttgacct tgagctccac ctaatggttg atgtatcatt atttcactat
    13681 ttggtagtgc tagtcttttt ccttttgctc ctgctgccaa caagaatgct cccatagaag
    13741 cagccatacc tatacatata gtacttacat ctggtttgat gtattgcata gtatcatata
    13801 tagccattcc agaagttata cttcctcctg gagaatttat atataaatgt atatctttgt
    13861 ctgggtcttc agcttctaaa aataatagct gtgctactat aagccctgct gtggcatcgt
    13921 taacttgatc tcctaagaag attattctgt cttttaatag tctagaaaaa atatcataag
    13981 atctttctcc tcttcctgtt tgttcaacaa ctacaggtac taatgccatg gtttcatacc
    14041 ccctttattc tatataattt acagtagctt tttttaagct actgtaaatt attatacgca
    14101 tttaactaaa ttatatttgc acatcattat atactaaaca tttcataacg tcaactatat
    14161 tagttagcgt ttattttatt aagctattgt tgcattatca actaataaat ctattgtttt
    14221 tcttattttt atttgacctt ttatatcttc tttgtcagca tctcttaaag aagcttctat
    14281 tttttctact tccatattgt aagcagaagc cattttttct aactcagcct taaattcttc
    14341 ttctgttgct tcaattcctt cagctttagt tattgcttct aatactaaag aagacttaac
    14401 taatttttta gcatcttctt ttctctcttc tcttaattct tccatagttc taccagtcat
    14461 ctctagtaat tgttgtaatc caaatccttg atattgtaat tggtagttaa gttcatttaa
    14521 catattatct atttgatgtt gtatcataac ttctggtatt tctatttctg tattttcagc
    14581 aactttttct actactgaat ttctagtttc tgcatctgct ctatttttag cttcttcttc
    14641 taacttagct cttacatctg cttttaattc atctaaagaa tcaaattcac tagtatcttt
    14701 agcaaattca tcatcaagtg cagataattc ttttactttt acatcattta tttttacatt
    14761 aaatacaact ggttttccag ctaaatcttg tgagtgatat tcttctggga aagtaacatt
    14821 aacttctact tcttcaccag cttttttacc taataattgt tcttcaaatc ctggtataaa
    14881 tgtgtttgaa cctataacta agttataatt ttcaccttta ccaccatcaa aagctacacc
    14941 attttcaaaa ccttcgaagt ctattatagc tgtatctcca tcttctaaag ctttgtcttc
    15001 tacacttact aatctggcat ttttttcaac catttcttgt agtttagcat caacattttc
    15061 gtcacttaca gtattctcaa ccttagctat ttctataccc ttgtagtttc ctaattcaaa
    15121 ctctggttta acttctacat taagaaccat aaccaaccca ttgtctttac ttatttcttc
    15181 tatatctaaa tctggattgt ctattgggtc tatgtctaat tccttgataa cttctggata
    15241 aacttctggg aataatattt ctatagcatc attgtagaat ataccttttc cgtattgagt
    15301 ctctataaca attcttggag ctttaccttt tctaaatcca ggtatattgt atttatttct
    15361 gcttttatta taagccttat ttactgctgc ttcaaattta ttgttatcta ctgttatttt
    15421 taaagtaact ttattaccct cttttttaat tagttctgct ttcatccttc taatcctcct
    15481 agtgatattt atgtaatacc cgataacggt attataattc attctattta taattcaacc
    15541 acttaattat atcacataag tttttttatt tacaagtgtt ccattaatat cgttgtactt
    15601 atttgataaa atttaatttt ttgagcgttt tttgccattt ttttagataa atacaccttt
    15661 atatgcgtat ttcttcaatt tttatgtcat cttcttctat atccataatg acaaaacttt
    15721 tatacgatac tcctcttgga agtgaagtgc ttccaggatt tatgtaaagt acaccatctt
    15781 taatttctct aaatggggta tgggtatggc caaatataac tatatcagca tcaacttcag
    15841 ttgccttttt ttctaacata ttagttccat attttacacc atacttatct ccatggcata
    15901 agaatatggt tttatttgcc acttcaaaga caacttcttc ctcaacagca tcaaagtcac
    15961 aattaccttt aactgcaata atacctacat tagtcatgct atgaatatat cttgaatcag
    16021 taaagttatc tcctgcatga attattaggt cacattcttt caaatatggt attgtttttt
    16081 ccatattttt aatcatcata tgtgtgtcgc ttactatgcc tattttcatt acttcactac
    16141 cctttttata aactcttgtt tcataagttt tagtgcgttt gctctatgac tgattgaatt
    16201 ttttataaca ctaggtattt caccaaaagt cttatcatat ccttttacta taaataagct
    16261 atcatatcca aaaccatttt tacctttttc ttcaaatcct atcattcctt cacaaattcc
    16321 tcttactaca aattcttttc catcaggaaa aactactgct attgcagaga cgaatcttgc
    16381 atttcttttg ctcattggta cattttgcat agcttttaac agtttttttc tattttcttc
    16441 atctgttgca tgttctcctg catatctagc tgaataaaca ccaggttttt tgcctaaagc
    16501 atctacttct aagccagaat catctgatat tgctataagt ttagtctttt ttgctattgt
    16561 tctagctttt atcaacgcat tatgctcaaa tgtttttcca tcttctacta tctctatacc
    16621 tgctaaatca acatctttta aagagtacac tttatattca aaatctttta agatttcacc
    16681 tatttcttct agcttatgaa cattattagt agcaatcact acttcattac cataatccat
    16741 acccaatatc tcatctgata tttcacctaa agcttctctt tgaagcctta ttaattcttt
    16801 attccctttt tcacctaact ctaacaataa atttaaatct gctctcgaaa aaggtttctc
    16861 ttctcctgtc ccctgtactt ctacaaactc acacttatca gtcattatta tattcatatc
    16921 tacctgtgct ttagaatctt cttcataaca taaatctagc atgtgtattc catttacaat
    16981 accaacacta attgcagaca caaaatttgt tattggcatc tcttttattg tctttgcttt
    17041 atataattta tatattgctt ctgcaactgc tacaaatgcc cctgttatag atgcagttct
    17101 agttccacca tctgcctgaa tcacatcaca atctacccaa atagttcttt ctcctaattt
    17161 attcaagtct acaacagacc ttattgctct tcctattaac ctctgtattt cttgtgttct
    17221 tccatcaatt tttcccctag atgcatctct tatctttctt tgttgtgttg ctcttggtag
    17281 catagaatat tcagcattta tccatccagt accactattt cttaaaaaag gaggaacttt
    17341 ttcttccact gatgcagtgc atataacctt tgtctctccc atttctataa gaacagaacc
    17401 ttctgcatac tttgtataat ctctagttat ctttacaggt cttatctgtt cattacttct
    17461 atcataactt ctggtcattt tttatatcct ttctcatatt ttttattttt tataaattta
    17521 tttttataat ttttaatttt gtcaaatatt aattattgtc atttttcgta atcttattag
    17581 cttactgaac tattctatca ttaattatag tagttttcta ctaaattaag cgacttttta
    17641 actctctaat acagctttta cttcatcttt cgatgataac tttaataatt ctaaaaatct
    17701 ctcttcatct ataactttaa ttcctaaatc atttgcttta gtaagtttag aaccagcttc
    17761 ttcacctgcc aaaacaaatg ttgtcgattt actaacacta gatgtagctt ttccacctct
    17821 tttttctatc atttcttttg catcatttct ttttaatgta ggaagtgtac cagtcaatac
    17881 gattttcatc ccttcaaata ttttttctac tttttcacct gtattatcca atgattttgt
    17941 atttactcct acatttttta acttgtttat aacgttcata tttttgtctt ccttaaaaaa
    18001 ctcgacaacg ctgtttgcca ttatacttcc aaattcctct aattcttcta gttgctcact
    18061 agttgcagaa attacttcat ctaaatcctt aaagttatat gcaagtatct tagctgcttt
    18121 aactcctata aatttaatac ctaatccatt tataagtctc cataaatcat tttcttttga
    18181 tttttcaatt gaatttatta aattgtcagc agatttttcg cccattctct ctaggtcgac
    18241 tacatcttcc ttttttatat aatataaatc agatacatct tgaataattt tttcatttaa
    18301 tagcagtgta attatagatt ctcccaatcc atctatattc attgcatccc ttgacacaaa
    18361 gtgtataatt ccccttctta tttgggctgg gcaagacata ttgatacatt ttacagcagc
    18421 ctctccttct aatcttattg ttggctctga acaaactgga cacttttctg gcattttaaa
    18481 atctatttca tctccagtac gttcttcttt tataacttct aaaacttgag gtattatgtc
    18541 ccctgctttt tgaactaata ctgtatcatt tatttttata tctttttctc ttatataatc
    18601 ttcattgtga agtgtagctc tacttacagt ggtacctgca agtctgactg gttcaagaat
    18661 tgctgaaggt gttattgtac cagttctccc aacttctact attatatcta ctattttagt
    18721 tttcttcttt tctgctggga atttatatgc aattgcccat ctaggacttt tggctgtata
    18781 tcccatttcg tctctttgtt ttatattgtt tacttttatt accataccat ctatttcgta
    18841 gtctaaatta cccctgttat ctgtccagta ttcaatatgc tcaataactt catctatact
    18901 tttacaaact ttataactct gacttactga aaatcctagt ttttctaagt actctagtga
    18961 ttcactgtga ctttctaaat cgtattcttc tatattttcc atattaaata caaatatatc
    19021 taatggtctc tttgctgtta gttttgggtc taattgtctc aatgaaccag ctgcaagatt
    19081 ccttggatta gcaaataatg gctgttcttg ttcttcttgt tgttcattta cttttctaaa
    19141 attatctttt gatatatata cttctcctct aactattagt tcttttttat catttatctt
    19201 aagaggtatg ctttttactg tcattaagtt ttgagaaata tcttctccta caactccatc
    19261 tcctctagtt gctccttttt caaactctcc attgttataa gttagaccta cagaaagacc
    19321 atctatttta aattcaacaa catattcaac attttctccc acatattctt gaactctctt
    19381 atcaaaatct ctcaaatctt gctctgaaaa agcatttgat aaactcaaca taggagtttt
    19441 atgtacaatt tgattgaact tatctaatgg cttaccgcct accctatttg ttggagaatc
    19501 aattctcttt agttctggat gttcttcttc taaagaaatt aactccttca ttaacttatc
    19561 gtattcataa tctgatattt ctgggctatc ttgattataa tacctttcat tatggtagtt
    19621 tattgtctct ataagtttat taatttcatc ttttacgctc actttttgca cctcttcata
    19681 tttttaaaat taaattattt tgaaatttcc ctataattaa ttatctccta tttatatatt
    19741 ttttagtata tcactttttt ggctttaaat ttattacaaa aaaactctta ttagtatctt
    19801 taatacgact aataagagtt tttattttta tcaaaatttt ttatttttaa tagcctcttg
    19861 atatgtctac aacattatct acaggtttat tctccattat atagtgtttc aaattattat
    19921 aaaccatatt aaatgttctc tctttgtttt tatctgacac ccaagaatta tgaggagtta
    19981 ctatgacatt ttcgcactcc cacaacttat tgtccttgtt taaaggttca ctttcaaata
    20041 cgtctaatgc tacccctcta aatttctcta cacattcttc taaatctttc tgattaactg
    20101 tatttcctct acctacattt ataaatacag aaccatcttt cataagttta aacatatctt
    20161 tgtttactat tccatctgta ctttttgtag aaggcattgc tacaactact atatcacatt
    20221 cttcaaatac aatattcata tcctcagtag caaaacactt atcaaagtat tttttactac
    20281 ttccatcagt attgacaccc catatttcta caccaaaagc ttttaatctt tttgctgctt
    20341 ctgaagatat agtacctgtt cctaaaaatc caactctttg tccatacaat tctgatacag
    20401 aaaaatccat cttccattct ttattttgtt gttgtttaaa aaattttgca ctatttttat
    20461 atatttctaa aatatacaat acaatacttt caccaatagg tacactatat ccacctctat
    20521 tattagctat cttaatattt ctcttcaata cctcttcttt tggaagttgg tctataccta
    20581 cactagttgt ctgtatatat tgtaaatttt tcatcttaga aatatctaat ctctcaaatg
    20641 gattgtatgt aactagtaca tctgctgtat ttacatcctc attattttca atactatctt
    20701 cagagtaatg tatgacatca tagcctaatt ctcttatttt atcaaatttt tcttttccat
    20761 aatttattgt aaataaaact ttcatctcaa atctcctcca tctacccaca tttaaaattt
    20821 tatattctaa aatataatat gatactcaat gtacattagt taaaattact ataagatatc
    20881 ttaaatttac ttatgttcaa ttgtttttcc taaagacata gcattacgtt cttgaactaa
    20941 attttctatt aaattattca atgtccaatc aatagatgtc tctgttacca tagctgtaaa
    21001 aggtctattt attctcactt tttttagccc ttctaaaaat gcatttactt gtgtgcttgg
    21061 agtgttatta aatattacag acttactctt aatcccattt tcacattcac ttgatatatt
    21121 tccatctaaa atgtctctta ttatattctc tccattttta tcacttaaga tgatatggtc
    21181 tcttattcca gcaaagttac ttacattttt aattaatgat aattcttttt tattaaagtt
    21241 tacaagcata atacacttcc tgttgtgtat tttatctagg ttattattat ttattttttc
    21301 aaatgacatt ttatccatcc tttcatttat ttatatactt taatttaccc catacattta
    21361 aaataatcaa tttttatagt taatttttat attaataccc aaattatatt taaatagtta
    21421 ggctatccaa aactaatata taaaattaga ataattagat taatatctac aaatcataac
    21481 ctaaaggaga gataccatga atacattact aaacaataaa tttagcatta aaaacttttc
    21541 aaatgcttgg gttatagtac ttggttgtat gttgatacaa gctgttccat ttagtattgt
    21601 agcaagtata cagcctcaat ttataagtta tgttgtagaa ggagaaggct ttagtttagc
    21661 ttctttttct ctaatattca ctattggtac tattgcctca gcaattgcat caccttttat
    21721 aggtgtatta ttttctaaag tcaatattaa gatactatat ttattaggtt gtataatttc
    21781 tagtattgct tttgctactt tttctatatg tactgaacta tggcaatttt acactgtatc
    21841 atgtattgtt caaattggcg ctacaattat atctgctata ggaatacctg ttttaataag
    21901 tgcttggttt gatgaagaaa gcaaaggaaa agctttgggc tttgcttttg ctggtggttc
    21961 tattggtagt atatttctac aaccacttgc agttcgctta ttagcaactc aaggatacag
    22021 tcgttcttat ctaatatttg gaattatagc attaatagtt ggtatacctg taatattatt
    22081 catactcagg atgcctaaag atattaatga aaaggtaaaa tctaaaggta aaaaagagaa
    22141 agcatctgat acaatttctc cttcagtatg gggttataca ttaaaagaag ctaaaaaaat
    22201 taaatatttt tggattatga ctattggatt tatatttgtt ggtatatatg tttcatcatt
    22261 atccgtacaa tatccaactt atcttagaaa ttatctaaat cctattctaa taggaacagt
    22321 aggctccaca tttgccttat ttgctctcat tggtaattta tttggaggat tattgtttga
    22381 taaattcggt caaacaaagc cacttattat atcttttgta ttggttctta tagcaacttt
    22441 atcattacta ttggtagata aatcttctac ttttgcattt gtatttgcca ttttgaaagg
    22501 tctttctgta tttgcttata tgattggtcc tgcttattta actggctctt tctttggtca
    22561 aaaggagttt ggtagcatat atggaatcgt acaattaatt tttgcagtgg gatttgcatt
    22621 aggttcaaca ttatttggat tgttagttga taattttgga tacagatttt cttggtctat
    22681 tataatttta tcagttataa ttgcctatac tttacttatt ataacttcta taggaatgaa
    22741 aaaattgaat aaagaaagat tagataaact aatgcaataa ttgattttta gatatataaa
    22801 tattataata aaaaagatgg agcttttaat cataaataaa actccatctt ttttatatta
    22861 caatatagct aattttctct tatttttaac tttactatct atatcctcat ccttaactat
    22921 agtaaatgcc ttagttggac aaacatttac acatgcaggc ccttctggcg attctataca
    22981 aaaatcacac ttatttgctg tcattctttc tctaaagcaa agctttcctt catctacatc
    23041 ttttaatgta actatgtcta ttgcacctat aggacatgct aacatacaag tcttacatcc
    23101 tatacaattt tcttcattta tttttattgt gttaccaacc ctaactatac caccatttgg
    23161 acatgccttt gcacaaggag catcttcaca atgtctacac tgtattggag cacttacttc
    23221 ttttgtcttt atgacagaaa gttttggatt aaaatttatt tcatctggat tttggtaaaa
    23281 aatatcttca tctgaatgtg caactacaca tcctatcata caagttctac atcctatgca
    23341 tttatcaggg tctgctataa caaaatagtt catcacttag ccacttcctt tcctatacaa
    23401 tatgtagtgt gtaatagatg atgagatttt tctcctaatg gttctaccag atattcatca
    23461 tacagttttt taatatcagg attctcatga gattttctaa ttgctaaatg ttcatcatgc
    23521 acgtatgttg cttttgttct attttcataa gccaattcct tatattcttc tagtaaaatt
    23581 tttggttgac ctccaccact tacacaccca acaggacaag tcataacttc aataaaatct
    23641 acatctaatt ttcctttttc caagtcttca agcactggta ttacattttt taatccactt
    23701 actactccaa ctcttaagtc taagtctcct attttaatag ttgactttct aaaaccattt
    23761 tctcctctta cttgttttac ttctacatca ggaatacttt ctccagttat aagctcatat
    23821 cctgttctta tagcagcttc cataacccca ccagtaacac caaagatagt tccagctcca
    23881 ctataactac ctaaaggact atcaaatttt tcttctctta aatttttaaa atcaattccc
    23941 atgtctttta ttaaataagc tagttctcta gtagttaata ctacatctac atctctatat
    24001 ccactagaat ccatttcttc tctatcacat tcatattttt tacatgtaca tggcattata
    24061 gaaacactaa atatctttga tgcatctaca ttatttatct ctgcaccata agttttaaat
    24121 attgcaccac ccatctgctg aggtgattta caagaagata aatgattagt taatttagga
    24181 taattttttt ccatgaattt tacccaagct ggacaacaag atgtaaacat tggaagagtt
    24241 ccaccttctg tcactctttt aattaattca ctaccttctt ccattatagt taaatctgct
    24301 gcaaaattag tatcgtagac tctatcaaaa tttaaagctc taagtgctgc tgccattttc
    24361 ccagcaccta agtatcctaa ataatatccg aaatcttctc ctaaagcaac tctaactgct
    24421 ggtgcacatt ggaccatagt gaatacattt gggtcactta aagctttctt caccttatct
    24481 atatcacata cattgtatgc tgcaaatata ggttcattta tagactctgg tatctctctt
    24541 tcttgttttt tctcttgtaa aaactcaaat ccttcatcta taactgaggc ataagattta
    24601 caagtttgaa cacattgacc gcacataaca catctatcta aatttatctc ttgtggcttt
    24661 ccctcttcac cttcaatagc atttactgga cagacctttg aacattctct acatccagta
    24721 cataaatcct tatctatatt tattatcata aacgtcacct acccctgtat cctttattta
    24781 tttgttaaca ataaactaag tgcagcttgt ctattcttag cttttttgtc atctattgga
    24841 gtaacaagtt ttaatgcttc ctgaggacat gcacttatac aagctatttt ttcattgtct
    24901 ttacataagt cacatttata agctatcttc ttattttcat ctaaaattac ttgttctact
    24961 tctttaccat cttcatattg tggaagtaag tctatagctc caaaagggca tgctagcaga
    25021 caagttttac atcctataca cttttcttca tttacagaca taatattgtt ttctttaact
    25081 atagcttttt gtggacatga gtttaaacaa ggagcatctt cacaatgtct acactgtatt
    25141 ggcatacaga aattgtcacc ttttactaag taaagtctag gtataacagg tatatcaact
    25201 gttccaactg tataacccac attattatgc tgattatgca ttgtaaaaca agctacttca
    25261 caagctctac aacctgtaca tttgtttggg tctgctatta caaaagagcc tattttattt
    25321 ttcatattat cccacctttc aatttgtata tttccaaccc tctagttatt ttcatcatat
    25381 gtctacttac tttcatcata tatatttata tatttcacta ttttctcagt acttatattt
    25441 cgtttacatt ttttgcacaa attgtatgtg ttattataca ccttatcagt atcatcaatt
    25501 aatactccac agatttcaca ctttgaatat ctttgctttt cttttttagt acacatatat
    25561 tcattctcac tttttaataa ctctctgtgg atttgcataa atattcatcc gtcttcctct
    25621 tacaaatcca ataagcgtta tatttaattt tttagaaagc tctattgata aattagtcgg
    25681 tgctgatttt gatactacaa taggaatttg agtcatagct gcttttaata tcatttccaa
    25741 tgatattctt ccacttacag ccacaatttt atcttttaat gttatgtcat ttaaaacaca
    25801 aaatccaata gccttatcca ttgcattatg ccttgctaca tcttcacaag tgactatttt
    25861 tttatcaaaa ttaaatacag atacgctatg taccccacca gtatttttaa ataattctga
    25921 tgaatttaaa tttttatcca ttatactgta aatcgtatca taatttattt ttatattgct
    25981 ctttacagga gcacatgtta tatagtctag tgaatttaaa aatataattt gctcatcttg
    26041 atttttttgt ttctcttgaa tagttacttt tgcaaaacgt tcactctcat ttatctcaaa
    26101 ttttaataag ctttttttgt tttctatgta tccttttgtt ttcaagaatc ctactataag
    26161 ttcctctaaa ttatatgggg tacataaaaa tgtatttaca tatttatcat ttaaaataaa
    26221 actaagtgga tattctgcaa ttatttcttc attatctatt gatacttcat gttcatttat
    26281 ttttcttact tctacattaa aagaatttaa atatttgtag tccactagac tataactatt
    26341 ttcaaaaact ttatccttac tattttcttg taatttagtt tttgagtata tattgttcat
    26401 aaaatattag cctttctgcc ttttataaat cttaaatcta ataaatcatt agatatttgt
    26461 agctgtcatt ttgtctttca aactttgata ctcttctctt acacattttt ctgctaattc
    26521 ttggtcttca agtttttcaa gtttaacagc acaatattta tactctggtg ttttagatat
    26581 agggtctaaa tttgctattg taagttcatt gcaagctcca acccaccact gataagtcat
    26641 atatgttgca ccttccttaa ctctatcagt aactgttgct cttgttatta cacttcctct
    26701 tcttgaacta attctaacta attcatcatc ttcaattcct aatttttcag catcattaga
    26761 atttatttga acacgtcctg gttcatcttc taatgaacta agtgttctac aattaccagt
    26821 cattgttctt actgaatagt gtccaacttc tcttacagta cataaactaa atgggtattc
    26881 atcatcttca acttccatag ggggtctcca ttcagcagcg aacaaattac ctttaccatt
    26941 aggagtagaa aacttttgac cttcatacag atacatagtt cctttatctt ccatactttc
    27001 actcttacaa ggccattgaa cgcatccttg agtttctatt ttttcataag tagcacctaa
    27061 gaaacttgga cataattgtc taagttcgtc ccatatttcc ttagtatttt tataattcat
    27121 aggatatccc attgctgtag aaatttcgct tataatttgc cagtctggtt ttacatctcc
    27181 ttgtggctct atagcttttc tcatcaattg gaatcctcta tctgcacatg tatatactcc
    27241 ctcatgttct ccccaagaag tagctggaag tataacatca gcgtgaagtg ctgtcttatt
    27301 cataaatata tcttgaacaa tgacaaattc caattcatct aatgcttctc taacttctga
    27361 tgcatctggg tcactttgca ctgggtcttc tccaaatata taataagctt taagcttttt
    27421 ctcttttagt actaaatttg gaacttgagt taaactataa ccattattag gagataattt
    27481 taccccccaa gctttttcaa acttttctct tatcttatca tcagttacat tttgatagcc
    27541 aggatataca ttaggaagag ctcccatatc acaagctcct tgaacattat tttgtccacg
    27601 tacaggtcct attccaacgc tttctctacc aaaattacct gtaagtagag caattgatgc
    27661 aagtccctta actacatcaa cagcttgacc aaattgacag acacccattc cataaagtat
    27721 catagctttt ttaccttttg catattctct catagctttt cttataagct cttctgggat
    27781 tcctgtaatc ttttcagcat atttagctgt atatggttta acaatttctt tatactcatc
    27841 aaatccttgt gtatgatttt gaacaaattc tttattgtaa agaccttctt ctattaatac
    27901 atttccaaaa gcatttacta aaaccatgtt tgttccacct tttataggta gatgtatatc
    27961 agcaattctt gcagattcag ttactctagg gtcagtaaca attaatttag caccattttt
    28021 tttagctttt actattctat ttgccactat tggatgtgaa tctgctccat tatatccaaa
    28081 tataaataaa acatctgcat tttctatctc tggtatagaa ttagacattg caccactacc
    28141 taatgagtaa gccagaccgg ctacagatgg tgcatgtcag acacgagcac aatggtcaac
    28201 attattagtt cctattgtag ctctcataaa tttttgcatt atatagtttg cttcattacc
    28261 aggtcctctt gcagaacctg ttcccataat agaatcaggt ccatattttt ctttaatttc
    28321 atttagtctt gaagctgtat agtttatagc ctcatcccat tcaacctctt ctagtacacc
    28381 ttgttttctt atcattggct tttttagcct aggtgttaga attttagggt catttagaaa
    28441 atcccatcca taacgacctt ttaaacacaa actttcttga tttgttctac cattagccgg
    28501 ttcagctctt aatatcttat tatcttttac tactagatat aattggcatc cagctccaca
    28561 gtatggacaa actgttaaaa tttttttctc catacaattc cccccttaga tttatcccct
    28621 gataagtaaa atttatctta aagataatga gtgtttttat aatatgcaaa atcataaata
    28681 ttttgcaata ctatctatta aattcaacaa ttttctcaca attcctctta aatttcaaaa
    28741 aaaatatgtc ataataatct ttatatttca accatttaat taaaatattc tagttcttat
    28801 gacatatttt ttaatatact caatatatat tctttaaact tatttagttt tcacttaaaa
    28861 caatatttct gccaattcta tttttcattc tatagtgagt aattacagta aatattccac
    28921 ttccaatcag aatcaaatag tacataaatc caccatataa aaatactgca tatcccatca
    28981 aaggttgagg gaatactgat ttgaatatag tgaaaaaggc taattcatta gctcctacat
    29041 tcccaggtgt tggtattgga gatattgcca tatataaaaa tgcttgtaat gtcagtatat
    29101 aaatatagct ataaccttgt aaattaaatg ctttatatat ccaaaatgaa acactaaagt
    29161 aagcacttaa ttgaataaaa gttactatta tagttgaaat taataccttt ttattctttg
    29221 caaaaaaatt tattgcttta ctataatcat ctataaaact ttctatggat tcaatcttat
    29281 tatctaaaaa ttttaaaaac ttaatttttg ataaatgctt tatagtaatt ctcataaaat
    29341 attttatttt tttaggattt aaaataacta atattatcat aattaagaca aatgaattaa
    29401 tggctatccc tagaaacact agaggcataa tcattttcat ttgtttttct aacatagcaa
    29461 aattcattaa aacaagtatt gtacaataaa aagtaactac tatttggaag ataatagact
    29521 tattggtaac aactgcactt gccttactaa gtggcatttt acatttttta agtacatata
    29581 tctgtactgg ttggcttcct gaagcaaaag gagttattaa attataataa aatcccataa
    29641 tggctagctt aaaaccaata aatctaatat taactttgtg agtgctttct ataattatct
    29701 tcattactac tccttcaagc attatatgac acattattgc caaagcacct atgaataaaa
    29761 acttcttatc taccataacc ataacatttg atagcatttt aatatccaat gttttaaaca
    29821 ctaggtaaat tgttatacct attaacaaaa ataaaaatgt atactgtagt aaactctttc
    29881 taatattctt tagtctatcc attagaaata accatccttt gctcaagatt tatactaaat
    29941 actatattat tagtatacac tatgaatctt aatattttat aacaaaatac cttacaattt
    30001 tgtcatttac aaaaaatatt aatctataaa ttaaatttta atatatagat taatatttta
    30061 caaacatttt gtagatatat ataatatttt cactaaacaa cacttatact tattacaccc
    30121 atttataccc tatcccccat acagtttcta ttgtataaat atcatattgt tttaatttgc
    30181 ttcgtatatt ttttatatgc tctctgatag tacttatatc actttctcca tcaaaaccat
    30241 ataccgactc atatattctt tcttttgaaa atacttgtcc cttatttttt gctagaaatt
    30301 cacatattag atattcactt ttagtaaaat taattttttt accatcaatt gaaacttctt
    30361 tgcctaaaaa gttaaattga acatttgaga tagttaataa attctttttt tctctacttt
    30421 ctctccttaa atgagcagtt atcctagccc ttaattctcc tacaccaaaa ggtttggtta
    30481 tatagtcatc tgcacctata ccaagaccgt acataatatc actttccata ttttttgctg
    30541 tcaaaaacag tattggacaa tcaaccttat ttcttatagt tttacatact tcaaaaccat
    30601 ccatcttagg catcataaca tctaatatta ttagattaaa ataattaaaa tccatgttca
    30661 tagccttttc tacatcacta attaccttta cttcatgtcc atctcttttc aacacatttt
    30721 caatcaactt taacatatct aactcatcat ctactactaa tatcttagac ataataactc
    30781 ctccatttat ccatctaata gcattctaat ttatatattt aataatatct ttaaaatt
//
LOCUS       DAEMIK010000032        30300 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEMIK010000032 DAEMIK010000000
VERSION     DAEMIK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.032"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..1144
                     /locus_tag="KPK87_001242"
     CDS             32..1144
                     /locus_tag="KPK87_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG2537409.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLICAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KPK87_001243"
     CDS             1398..3422
                     /locus_tag="KPK87_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2537410.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KPK87_001244"
     CDS             3642..4328
                     /locus_tag="KPK87_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG2537411.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KPK87_001245"
     CDS             4423..5478
                     /locus_tag="KPK87_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG2537412.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="KPK87_001246"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="KPK87_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG2537413.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="KPK87_001247"
     CDS             7195..8901
                     /locus_tag="KPK87_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG2537414.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFKEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="KPK87_001248"
     CDS             8923..9996
                     /locus_tag="KPK87_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG2537415.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="KPK87_001249"
     CDS             10008..10730
                     /locus_tag="KPK87_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2537416.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="KPK87_001250"
     CDS             10746..11903
                     /locus_tag="KPK87_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG2537417.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="KPK87_001251"
     CDS             11906..12745
                     /locus_tag="KPK87_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2537418.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="KPK87_001252"
     CDS             12760..13935
                     /locus_tag="KPK87_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG2537419.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="KPK87_001253"
     CDS             13964..14713
                     /locus_tag="KPK87_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2537420.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="KPK87_001254"
     CDS             14735..15643
                     /locus_tag="KPK87_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2537421.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="KPK87_001255"
     CDS             15691..16443
                     /locus_tag="KPK87_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2537422.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16421..17764
                     /locus_tag="KPK87_001256"
     CDS             16421..17764
                     /locus_tag="KPK87_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG2537423.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEDLGIEYYSIGR"
     gene            17770..18861
                     /locus_tag="KPK87_001257"
     CDS             17770..18861
                     /locus_tag="KPK87_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2537424.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKKAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="KPK87_001258"
     CDS             18945..20858
                     /locus_tag="KPK87_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2537425.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFMATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIADLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="KPK87_001259"
     CDS             21057..21764
                     /locus_tag="KPK87_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2537426.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="KPK87_001260"
     CDS             22066..24177
                     /locus_tag="KPK87_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG2537427.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="KPK87_001261"
     CDS             complement(24375..25340)
                     /locus_tag="KPK87_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG2537428.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="KPK87_001262"
     CDS             complement(25625..26581)
                     /locus_tag="KPK87_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG2537429.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="KPK87_001263"
     CDS             26949..27737
                     /locus_tag="KPK87_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2537430.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="KPK87_001264"
     CDS             complement(27860..28330)
                     /locus_tag="KPK87_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG2537431.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="KPK87_001265"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="KPK87_001265"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG2537432.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="KPK87_001266"
     CDS             29156..30061
                     /locus_tag="KPK87_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2537433.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHPNRKKRKLNKKKLAMLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQAVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12005 a         3301 c         5382 g         9612 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgtaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attgtttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tatctacatt
      481 tataatagtt gggtactaca tcttagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa gggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatat gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgaaata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaat gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccatagtaca aacttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataat aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagagtt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt aaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaagca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gctttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga acaatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaagat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggccg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaat tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcgacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctaatct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtact atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgacccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcagca tgttttttaa
    11101 tggtagctat attattattt aatctatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaataattt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccaa
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggcatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagatt atacaagatt tgatgagaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttgg gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagaa tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggctca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagagcttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa accagatact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga tttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt gaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aaacataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa ggctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagtgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttatgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taaaaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agtacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag atattattga tatatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt gaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 acttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gttatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtgcttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttgta tgaagaacta cttatgtcag
    20641 aagaaggtct attggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtaccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tataaatggg gagcaactgg accaaattct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaaa aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaggaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggataattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaag gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcggctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta caaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgcac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctgtgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc gcccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtctt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ttccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtgctat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca cgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagtattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactaatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atcttataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggtcagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaggg agacatattg aagatagctg tatttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat atctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatcgc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggatgc atttagttgg tatgatacac aagatgtcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catccgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcaatgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactagtcaa caacaaaatg ttaattcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacggca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag gcagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat tgatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 agttaacttt acttcaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgaa aacattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaatttaaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
//
LOCUS       DAEMIK010000033        28763 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEMIK010000033 DAEMIK010000000
VERSION     DAEMIK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.033"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(67..1293)
                     /gene="murG"
                     /locus_tag="KPK87_001267"
     CDS             complement(67..1293)
                     /gene="murG"
                     /locus_tag="KPK87_001267"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG2537434.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSASKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(1311..2441)
                     /gene="spoVE"
                     /locus_tag="KPK87_001268"
     CDS             complement(1311..2441)
                     /gene="spoVE"
                     /locus_tag="KPK87_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG2537435.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(2493..3848)
                     /locus_tag="KPK87_001269"
     CDS             complement(2493..3848)
                     /locus_tag="KPK87_001269"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG2537436.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(3867..4835)
                     /locus_tag="KPK87_001270"
     CDS             complement(3867..4835)
                     /locus_tag="KPK87_001270"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG2537437.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(4876..6249)
                     /locus_tag="KPK87_001271"
     CDS             complement(4876..6249)
                     /locus_tag="KPK87_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG2537438.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(6416..8395)
                     /locus_tag="KPK87_001272"
     CDS             complement(6416..8395)
                     /locus_tag="KPK87_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG2537439.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIEAFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(8407..8754)
                     /locus_tag="KPK87_001273"
     CDS             complement(8407..8754)
                     /locus_tag="KPK87_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537440.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(9235..10170)
                     /gene="rsmH"
                     /locus_tag="KPK87_001274"
     CDS             complement(9235..10170)
                     /gene="rsmH"
                     /locus_tag="KPK87_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG2537441.1"
                     /translation="MEFHHVSILLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(10200..10946)
                     /locus_tag="KPK87_001275"
     CDS             complement(10200..10946)
                     /locus_tag="KPK87_001275"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG2537442.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(11340..12626)
                     /locus_tag="KPK87_001276"
     CDS             complement(11340..12626)
                     /locus_tag="KPK87_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2537443.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(12641..13906)
                     /locus_tag="KPK87_001277"
     CDS             complement(12641..13906)
                     /locus_tag="KPK87_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2537444.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            14222..15319
                     /gene="ychF"
                     /locus_tag="KPK87_001278"
     CDS             14222..15319
                     /gene="ychF"
                     /locus_tag="KPK87_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG2537445.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            complement(15798..18077)
                     /locus_tag="KPK87_001279"
     CDS             complement(15798..18077)
                     /locus_tag="KPK87_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2537446.1"
                     /translation="MLSDAKNNRYGLFIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(18348..19802)
                     /locus_tag="KPK87_001280"
     CDS             complement(18348..19802)
                     /locus_tag="KPK87_001280"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG2537447.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVLDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            19966..20835
                     /locus_tag="KPK87_001281"
     CDS             19966..20835
                     /locus_tag="KPK87_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2537448.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(21048..23120)
                     /locus_tag="KPK87_001282"
     CDS             complement(21048..23120)
                     /locus_tag="KPK87_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2537449.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PVLANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPTIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(23423..24265)
                     /locus_tag="KPK87_001283"
     CDS             complement(23423..24265)
                     /locus_tag="KPK87_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2537450.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(24526..26568)
                     /locus_tag="KPK87_001284"
     CDS             complement(24526..26568)
                     /locus_tag="KPK87_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG2537451.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQKGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(26720..27691)
                     /locus_tag="KPK87_001285"
     CDS             complement(26720..27691)
                     /locus_tag="KPK87_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG2537452.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     QTLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(27692..28669)
                     /locus_tag="KPK87_001286"
     CDS             complement(27692..28669)
                     /locus_tag="KPK87_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537453.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
BASE COUNT         9336 a         5071 c         3041 g        11315 t
ORIGIN      
        1 aataaacccc taatttataa taatttagaa aatctaatat attataaatt aggggtaaat
       61 atttacttat cttttcttta ttcctattac cttagcttga ccttctatac ttggagtcgt
      121 ttcttttttt atttctttta cttccttaat aactttttct ttttttgttt ttttagaagc
      181 tgatttttgt gttgaattgt atactttcat tatctcatca tatataaggt ctatggcttc
      241 aggcttacct atagttttac ttgcatttgc catatcaacc aaaagttctc tatctcctaa
      301 aagtttaaat actgctgtat ttaggctctc tggagttaga tttttttcta aaatagcaat
      361 tcctgcccct tgtttttcta tactcttggc attatactct tgatgatttt cagcagtata
      421 agctttaggt attattatag atggctttcc aagagccgtt atctcagcta atgaaattgc
      481 tcctgcgcta cctataacca aatcacttgc tgcaagtgca tttgccatat cttctaagta
      541 aggcacaact ttttggtatg gtttaagatt tatatcgcta atacttccca taaattcatc
      601 ataatagctt ttgcctgtag caaatataaa agctatatct tcatttacca tattctttat
      661 tacaagtctc attgcatcat ttatttttct tgaacctcca ctaccaccat agcaaagtac
      721 cattcttttt tcgtcactta ttgatagatt ctttcttgca atattttttc ttgataataa
      781 aatttctttt ctaactggat ttcctgtaaa aactaattta tcttctgctg cctctggaaa
      841 tcttttatgc gaatcttcaa aacttgtaag tacctttgta actgttttag ataaaatctt
      901 attagttaca ccaggaaaag aattttgctc atgtattatg gctggtattt tccccataga
      961 tgcattaaat aaaacaggtc cactaacata tccaccagtt cctataacta tatctggttt
     1021 aaatttctta acaatctttc ttgattgctc caaaccttta aaaagcttaa aaactctttt
     1081 tacattatcg aaatctattt ttcgcttaaa accttgaact gttacagtct ttaattcaaa
     1141 tccatactta ggaactattt ctgactctat tcccttttcc gtcccaacaa aaattatctc
     1201 agcgtctgga tgctcatctc ttattttgtt tgcaatagct atagctggat acacgtgccc
     1261 tccagttcca ccgcctgata ataaaacttt catcaaatca tcatctcctg ttaattaatt
     1321 ttgacatgct ttgagatgtt taacactatt cctacagctc ccataaagat ggtgagtgaa
     1381 gttcctccat aacttataaa tggtagtgct acacctgtag ctggcattga tgatgtcgca
     1441 actgctatat ttaaagctgc ttgtattcca atttgtgcac ctatacctat aacaaccata
     1501 caagcaaata cattagaagt cttcaaagct attcttacac atctatagac taaaactaca
     1561 aatagcataa taactattat acaaccaatc aatcctaact cttctcctat tatagcaaat
     1621 ataaagtcat tttgtggctc tggtatataa aaatactttt gtttactctt tcccaagcct
     1681 agaccaaaca atccacctga acctagtgca tatagacctt gtattacctg atacccctta
     1741 ccaagagggt cttgaaaagg gtctaaaaat gatgtaacac gactaagtct atagggttca
     1801 gcaattacta atgcagcaaa taaagcagcc ccactcccta tcatagcaaa tacaattttc
     1861 atatccattc cagcaacaaa tatcattaca aaagtaacta gaattacagt acctgctgtt
     1921 gacaaatttg gctgaagtat tatcaacgca aaaaatatcc ctggaactac gagaagtggt
     1981 acaacacctt ttgtcaaaga ttttatttta tcataatttt tttcaattaa ttttgcagtt
     2041 aatattattg tagcaaactt tgcgatttct gcaggttgaa atgttaaagc tcctattccc
     2101 aaccatcttt ttgctccgtt tgcttctatc cctaaagggg tcaaaactaa caataataat
     2161 actactgcta tagctccaat tgctgtagca tttttcttcc aaaaactata atctattctt
     2221 gatgtaataa tcataactat aaatccaagt acagcatata ttacattctt ttttagaaaa
     2281 tagtacgcat cattgtgttt aaatgaagat tgtataaaac ttgcactaaa caccataact
     2341 attcctacaa atactaaaag catagtagtg taaaacacta ctccatcaaa ttcagtcttc
     2401 atccttatgt caatttgttt tttcaaattt tctttaggca tccaactaat ctacctccat
     2461 tcttccattt aagtaagttt agaggtaaga gattatttta aattgtttac attatctttg
     2521 aagtcttttc ctcttacttc aaaactttca tacatatccc aacttgcaca agctggtgaa
     2581 agaagcacta tatctccact ttttgaaatt tcataagaag tcttaactgc ttcttccata
     2641 tcttttacta tatgtatgtc attaaatcct ttatttttag cacaattttc tatatttgat
     2701 gcagtttctc ctaataagac caatgattta acatatgact ttgcagtttc aagaagttca
     2761 tcaaaattac ttcccttatc catcccccct gctataagta ttataggtct atcataagac
     2821 tgtatagctt ttatagtgga atctggatta gttccctttg aatcatttac atatattaca
     2881 ttgtctaaat ttcttacaaa ctcttgtctg tgttccactc ctttaaatgt cataagtacg
     2941 tcctttataa cttctaaatc aattttgcag acatatgcta ttgctatagc tgccatacaa
     3001 ttttctaagt tatgacctcc aggtaaactt acatcatctt tatttagaaa tttgatttta
     3061 tcatctatat ctattattat gtcattattt tctaaatata ctcctccatt tactttttta
     3121 gttcttgaaa agtaaattac tttggcattg catttatcag ctaaagattt tactatctca
     3181 tcatcatagt ttaatacaca gaaatcctca acagtttggt tcataaatat atttgccttt
     3241 gcttctatgt atttttccat agtatggtgt ctatttaaat ggtcctctgt aatatttaat
     3301 attgcgctta ctttaggtct aaattcatct atactctcaa gctgaaaact gcttagttca
     3361 gttactaaca cagactcttc acttgaagtt tctatagtat ctattacagg atttcctata
     3421 ttaccaacta catatgtatc tcgttttgct cttgaaaaaa tttctcctac caaactagtt
     3481 gtagttgtct ttccatttgt acctgtaatc cctataaaaa ttggattatt cgatagttta
     3541 aaagctaatt ctacctcacc aattatatac ttgcctgaat tttttaattt caatatgaat
     3601 ggtaggtcta gtggtacacc tggagacact acaaccatat ctatatcatc tacatcttct
     3661 gggtgatgtc ctaatatgta ttttatgtca tttatagatt tcaattcatc aagtatattt
     3721 ctcaatttat tttcatcttt tatgtcattt acgattatac ttgcacccaa tttatctaaa
     3781 tgtttaattg tagatatacc agtttttgca agtcctacta ataaaacttt ttttcctttt
     3841 aagtccattt ttcattcccc ctagccctaa aatactgcta ttatacttat ccaagctaaa
     3901 acaactgtta taatccaaaa aataaataca actcttgttt ctggccaacc acactgttca
     3961 aagtggtggt gtattggagc catcttaaag aatctctttc ttgttgcctt aaaatatcca
     4021 acttgaatca aaactgatag agcctccgca aaataaattc ctccaattat aggtattata
     4081 agcactgagt tagttagaac tgaaaatgct acaactgcac ctcctaatgc cattgaacct
     4141 gtgtcgccca taaacactct agccggatat gaattaaatc ctaagaaacc taaacatgct
     4201 ccaacagttg ctgctgctaa aactgcgact tctgtatttc cagcaataga acttgcaaat
     4261 agcataaaga atacagaaac tattaatgtt actcctgatg ctaatccatc taatccatca
     4321 gtcaagttta cagcatttac tattgctacg attataaaca tcattattgg tatatataat
     4381 attccaacat ttatcacaaa atctgtaaat ggtatcataa gttgtgaagc acttgatgat
     4441 gatgtatact gataaaaagc aacataaaaa gataatgcaa cttgaaggat tattttttga
     4501 tatgctttta gtccaagtga tctttttagc tttattttta taaaatcatc taaaaagcca
     4561 ataaaaccaa aacctgcaat acatataaga cctactgcca tatcatgact aacttttact
     4621 cttgtaagtc cagttattag aattgcaaca atcattataa ttccacccat agtaggtgtt
     4681 ccgtttttag ctagatgtgt ttggggtcca tcatctctaa ctgtctgacc aaatttaaat
     4741 tttctaagca ttggtataaa tattggtcct attataatta ctattaaaaa cgcaatcaat
     4801 gcggtgtacg taagctctgt tattcctaac atcataaaac tcctctccct tgtcaattaa
     4861 ctcattagtt taagcttatt tatttagata ttcgactatt ttttctaatc tcatgcctct
     4921 tgagcctttt actaaaatag tgtctcctgt ttttactaat tcatttaact tgttaaacac
     4981 atcttctcta ttctcaaagt gatatatatt ggctgaattg aatcctaatt gttttgcttc
     5041 ttctccaata aacactgaat tttcacctat agttattata atatcagtat tattcataga
     5101 tgattttcca actaatctat gaccatactc tgaaatttca cccatttcta gtatatctcc
     5161 aagtatcgct acccttctac ctttatatct tccaagtatc tttaaagcag catccataga
     5221 atctggacta gcattatatg aatcattaat tattgttaat ttttcatttt ttattatgtc
     5281 tagtctcatt tttgtccctt taaaattttt taaacctttt tttatataat ctaaagaaat
     5341 atttagacac aaaccaacta atatagcaga cattgcatta tatatattat gttctcctat
     5401 tgttggtata aatatttcct cttttttccc atttattaca catataaatg ttaaactatc
     5461 ctcttccata gtataacttt cacaataaac atcattgttt ttattaaagc caaatgtttt
     5521 taacttatat acatgttctt tttcttttaa tgtagataaa aacttatcat ctccatttac
     5581 aattaaagta tttgtttcat caaagtttga agtaatctcc atctttgctt tcaaaatacc
     5641 ctcttgtgaa cctagttttt ctacatgtga taatcctata ttcgatatga ctgcaatttt
     5701 aggatttact atatccacta aatattttat ttcattgaat ccagacatac ccatctctat
     5761 tacagcacac tcatgttctt tatttagatt aaataaagtc aatggaactc caaaatgatt
     5821 atttaagttt ttttcatttt ttagtatatt taactttgca gatatggcag agtaaatcat
     5881 atctctagta gtagtttttc ccacactacc tgtaacacct ataaatggaa tatcaaattt
     5941 ttctttatag tattttgaaa tatctcctag agccaaagat gtatctttta cctctattaa
     6001 atttatatcc gaactttcta attttattcc attactttca ttttttataa aagttttaca
     6061 tccactatca tatgcagact tcataaatgt atgcccatct aaattttctc caactatggc
     6121 tacaaatgca ttttgggcat ttgcctccct gctatctata actatatcac ttacacagtc
     6181 attataatct ccatatatta acttcccatt agttgcaagt actaactctt ttattgttaa
     6241 gcactccatt attaaactcc tcctttaaaa ccttagctat attctcttag caacaaaagg
     6301 caatgtatac cgttatatat acattgccta cgataactct taactttata attatatact
     6361 atatttctgt ttttttgaag atgtttatgg gtatatgtta attatctgtt actttttagt
     6421 tttcaaaata tagggttata cttgagtctt cattaacttt tactccaggt tttgggaata
     6481 tatcctttac ctttgtatct tctggaagtt ctatatctgc atctaagtct ggttttaact
     6541 tcgcatcctc caatgccttt acagcatctc ctatttttag atttcttaca tctggcactt
     6601 taacttgctt cttttcatac tcagctttct cttcttcttt atatactggt ttaactccca
     6661 aatattttaa agagtcattc attatttctt tcactattgg tcctgctgta gtactaccaa
     6721 atgcacttac tcctgttggt tcgtcaacaa tagcaagaac tactatttgc gggtcatcac
     6781 aaggtgctat tccaacaaat gaacatatat attttcctgg agcatactta ccatctataa
     6841 ctttttgagc tgtacctgtt ttaccaccaa gtctataacc tggtatatat gctatctttc
     6901 ctccaccttc agttacaact gattctgcaa tttctaacat ttttttagat gtttctttag
     6961 atataacact tcttactttt tttggtttta ctgtttcagt tatatttcct ttattgtcag
     7021 tataagattt taccactctt ggttgcatta aatctccacc attagctatt gatgatatag
     7081 ctgttataag ttgtattgga gttactgata tagattgacc aaatgatatt gtagccaatt
     7141 caactggtcc tacatttttt tcgttataca gtattccctt agcttctcct ggtaaatcta
     7201 tacctgtctt atccataaga ccaaatgctt ctatataatc atacattttt ccaacaccta
     7261 atctgcttcc taactcaaca aatactgggt tgcaagagtt ttgtactgct tgtttaaatt
     7321 cttgtgttcc atgaggtctg taatgtctcc aacatttaat ctttcttcct ccaacagtta
     7381 cactacctgt acatgtgaat ttttctccat ctttaattac tccttcttca agagcactag
     7441 aagatgtaat aagcttaaat gtagaacctg gttcatatgt atcacttact gctggatttc
     7501 tccacatttg ataatagcct tttattttat ctttatcatt atatttttca agttcttctt
     7561 gataatatgg atatattggc gttcttgagt cattagggtc ataatctggt tttgatgcta
     7621 aagcaagtat atcacctgtt ttagggttca tagctattgc agtaaccttt tttgcattat
     7681 ttaactcata agctttctgt actgcttttt cagtatagtg ttgaattact tcatctatag
     7741 acaacacaag accattacct tgtacaggct cataatattt ttccattcct tgtggtattt
     7801 ctcttcctga agcatcagta cttactataa gttttccagg ctttcctttt aactttttat
     7861 catattgcat ttccacacca gatatacctg ttgcatctga agaagtatgt cctaacacat
     7921 atggtgcaaa attcccatat gggtaatatc tttgattatc ttctgctacc catattccac
     7981 ttaatttagc atctcttatt tgacttgcct tatcatcatc tatccatctt tttactttta
     8041 caagagccat attttttttg cttattaaag cgtaaatatc tttataatct tcatccaaaa
     8101 tctcagctac cttttcggct gcttcttttt tatcttctac ttccacaggt ttacaccaaa
     8161 cagtatactt agttacactt actgctaact ctttcatatt tctatcatat atagttcctc
     8221 ttttgggttc tattggtata tctcttgttt gttgttctag tgcttttgta ctcaaccaat
     8281 ttcccttcat aagttgtaga tacccagtcc taataacaag gcaaaaaaac aatgcacatg
     8341 ctagaataag aacgagtacc agccttttct tacttatcct ctttactttt ctcaaatcta
     8401 ttccccctag ttatccacat ctatatactt tatttgtcca tcttttggat agtccatatt
     8461 gagcaattcc ttagccttta gctcaatttc ttttatcgat gttttttcag tttcttttgc
     8521 ctttagttcc tctaatacta tctctttttt tcttaaatct ttttgtaaat aatagatatc
     8581 atacttcatt tccgagattt ttgaataacc acatagattc aatattatta ctgaaaaagt
     8641 tgcaatcagt aaaatgcatt tagctcctat ttttttctta tttctctttt tataattatt
     8701 ctttttttta tttttatatt catttagatt atgtatttta taattttttt tcaaattctc
     8761 accccgatag tatatagttt aaattttcct ctatactata tttgttatta atcatatttc
     8821 ttcctctaaa atttcatcgc taaattaata cactattaca caatctaatt gtattttttt
     8881 ctatacacaa caaaaaccta taaattcact tatactttta tatgaatcta taggttagat
     8941 attaatttct atttattttt atcacttcta gtatttttag ataatttctt ccataaatat
     9001 aatatgtaac taactatcta ctctctgatt cctaagttct ccattatatt acttaatacc
     9061 atctatatct atatttttat atctaaattt tcattatata tctcaataac aaagaacatt
     9121 tcttatagtg ttaattgttt tttaattgct ttaccagtgc aacaaaatat ataatacttt
     9181 ttaattaatt ataataatct tatatataag attattcttt agccttataa ctacttatat
     9241 tttttctgct actcttagct tagcacttct tgaacgtgga tttacttcta tttcttcctc
     9301 actaggtaat ataggttttc tagttattat tttcacctca gattctttat cacattggca
     9361 tataggtaaa tgttgtggac atatacaatc tgatgctaaa tgcttaaatg catttttcac
     9421 tattctatct tctaatgaat ggaatgttat tatacatatt cttccacctt cattcattat
     9481 agaagaagca tcatttatca tctttgtaat tactccaagt tcattattta cttctattcg
     9541 tattgcctga aaagttcttt tagctgggtg aggtccatct attctagctt tcttagggat
     9601 agctttttta attacatcta ctaattctcc tgtagtttct attggtttgt ttgctctttc
     9661 ttcaactata aatttagcta tacgcttagc ccagttatct tctccataat cttttattat
     9721 cttagttaat tcatcttctg tataagtatt tacaacatca tatgcagaaa actcacacct
     9781 aacatccatt ctcatatcaa gtggtgcatc ttgcatataa gaaaatcctc tatcagcttc
     9841 atcaagttga tgagatgaaa ctcctaaatc agctaataca ccatcaattt tgtaaactcc
     9901 tattttttct aattcctctt ttatattttc aaaattactg tgaacaaact tcactctact
     9961 ttcatattca ctcaatctct cttttgctgt gcttatagca tttttatctt ggtcaaaccc
    10021 tataaataat cccttatctg acagtttttt tactatttct ttagaatgtc ctgctcctcc
    10081 catagtacag tcaacataca cgccatctgg ctttatattt aaattctcta tacactcatt
    10141 taaaagaatt gatacatgat gaaattccat agttacctcc cttaaattca ctttttaaac
    10201 tattcttcac taatattatt tttatttttc tttgataaat aaacatgtgc aattatgcca
    10261 ccaataactc caattagact tataacttgc gccattctaa gtggtcctaa cattaaacta
    10321 tctgttctta atccctctat aaaaaatctt cctattgaat ataaagttat ataagtaact
    10381 ataacttgac cttcatattt tttatttttt ctaaatagta ataaaactat aaatattcca
    10441 aaatcccata tagactcata taaaaaagtt ggatgtacct ttactccatc aaccattatt
    10501 ccccaaggta aattggtagc acctccatgt gcttctccat taataaaatt tccccatctt
    10561 ccaatagctt gtgctaaagg cattccaagt ataactgtat cagccatttt taagaagtta
    10621 atatttttaa tcttagtgta tatataacca gctaatattc caccaattaa ccctccatgt
    10681 atagcaagtc caccacctct aaaattaaaa atttgagaca tattttgagc atagtaactc
    10741 caattaaata caacataata gattcttgca cagattagtc caactggaat tgctattata
    10801 gctatattta atacatcatc ttctttaatt cccactcttt ttgcttcttt tattgctatt
    10861 aaagttccta aaatcattcc acatgccata agtattccat accacatgat gtctatgcca
    10921 aacagtgtaa atgctactct atccataacg tcacctcttt gtattaattg aaaatatttc
    10981 caaagcaata tgctataatt acaattaatt gtaattatac taaattatga taacacatta
    11041 atgttaattc tacaaaaaat attaaaattt attctacaac tccctacact atattatatc
    11101 taattactat aaaatttttt aaactgaatc tctcatgact aaagttacaa gtgtacatag
    11161 atatttttta atcaaaaaga ttgatgcata tatacatcaa cctcttctaa ttcttatcta
    11221 tagctcttaa atttttacct caatttatat aaatatattt tataaatttg ttttaccaaa
    11281 ataaacattt aaaaacataa actaatactt aattagttat tcttattatg attaagtttc
    11341 tatttaggct ctacaataga aataacacaa tactcttctt taaaatgctc ttttaatctt
    11401 tcttcaactt cctcaaaagt aatatcttta ataatatcta aataatccaa tagatttata
    11461 tccttaaaaa cataagatat aaaactatta cctataaagt ttatagaatc aaagcactta
    11521 ataaatgacc ctattttttt ctttttagtt ctctcaaact cttcttttga taatccttct
    11581 ttttttgatt tttctatata atcaagaatt atttctttta cttttttagg ctctttagaa
    11641 tctccagcta ttatgctaaa agcataatcc acttgagacg aaaatccagc tccaaaattt
    11701 tcatttatta aaccttgcat atacaaatct tcataaagct tacttccttt tttaaataac
    11761 atccctacaa gtatatcagt tacaatctct ttacgtaaaa gttctttccc ttttaaacca
    11821 acattactat ctttaaaacc tatgttgaac ataggcatag atatagggaa tttttctata
    11881 acctcttttt cttttacacc ttctggttct tctggataaa atctttctat actcttagag
    11941 agtttgtcta ctttgtaatt gtttgacttt ttagttacat ctataacttt ttctacatct
    12001 aagtcaccaa caacaaataa agccatattt cctggattat agaaagtatt ataacattta
    12061 tatagctctt ccttagttat cttatatata ctatcaacag tacctgctat atcaattcta
    12121 gcaggataat ttacatacat tgccttcaag caattaaagt atacattcca atcagggtca
    12181 tcattgtaca tttttatctc ttgtgctatt ataccttttt ccttttcaac attttcatct
    12241 gtaaaataag gtgtttgaac atagtcaatt agatgttcta aactctcata aaaattttca
    12301 gtagcagaaa ataagtaggc tgtcattgta aaattagtaa atgcatttgc atttactcct
    12361 aattttgaaa atttatcaaa tgcatctcct ccatcaggtt gctcaaacat cttatgctct
    12421 agaaaatgtg caataccttc attcactcta attttcttat cttctccaat tggtacaaat
    12481 tctaagtcat ttgaaccata attagtagcc aaaatagcat actttttcat aaaacccctt
    12541 ttaggcataa aatatacatc taatccattt tgcaatttct cataataaac ttcttctttc
    12601 aatatatcat taactatttt ttccatagac atctcctcca ttaatttctc aagaagtata
    12661 cagtatcaag ctctatattt tttatagctt caactatatc tttttttgta accttttcta
    12721 tactagatat tatatcttgt acttcagaat ttgtctttgc catagattga gagaaaacaa
    12781 aatcagacat accacctata ttatcagtta tagatttcat agaattaact aaagcaagtt
    12841 tactgttttc taattcttca tcagatatat cacctgcttt tacactttct acttgttttt
    12901 ttatcaattc tacagcttta tcataatctt tagtttctat accagatgat ataaacatga
    12961 tacttttata cttctctatt gaagagaata tatagtaaca taaactttct ttttctctta
    13021 cattaacaaa taatttagag tgtggaccac cacctaagac attgctccca actactaaag
    13081 cataatattt atctatgtct gcataatcca catttgctct ataacccata actaacttac
    13141 cttgagttat ttccatttgt tcatctataa cttttacctc atccacattt tttataaaat
    13201 ctgctcttgg tatatttata atttcttctc tttcaaattt tagattttta cttattatat
    13261 ctacaacttt atcttcatca aaattacctt ctacaactat atctataggt gatgttttaa
    13321 gtatgttttt ataatgttca tataactctc ttgaagttat actatctatt tcatcttcat
    13381 aaccaaattc acttacacta tatctctctc ctttacacat agcctcaata catttatctt
    13441 gcgcatattc ctttttatca tttattattg attgtatctt ttctcttaaa ttctcttttt
    13501 caatatttaa atattcttct ttaaacccac catctacaac aagcgtatta tttattactt
    13561 cattaaaaaa ttctactact tcttcaaata tactctcatc taaatacttt tcacttatgt
    13621 taattacttt aaaactcaat acttgacgtt ctccacgttt tacagcatca gctcccatag
    13681 atgaaccata taaatcatct aatttattgg atattgctct agcactagga tatttagcgc
    13741 ttccacttgc tattattgat ggtattagag cattttttgt agtttctttt tcatccaata
    13801 gtctctgaac atataaactt ataagattcg atttaaactt ttctgttttt atcaaagtaa
    13861 gattaatatt attacctaaa tttattattt ttttagtatt aaccatatgt acctcctatt
    13921 tttatttaaa catcaatttt aaaattgaaa tttatttatt ttttaattac atatactcac
    13981 atataaaaac cttctattta attatatcct ttttattatt ttctaaaagc aatattttta
    14041 tttaaattaa atacaaatat tatttaaatc aatatgcttt aaagtctagt ttacaagctt
    14101 gatatatatg atgtataatc aatatgtatg tctataatta aaacaatttt gcaatcctat
    14161 agattgattg tttaaagtta tataaagaat ataaaaaaga ataagagaga ggacgattaa
    14221 tatgaaatta ggtatagttg gtttaccaaa tgtaggtaaa agcacactat ttaacgcaat
    14281 aactcaagca ggtgcagaat ctgcaaatta ccctttttgt acaatagacc caaatgtggg
    14341 agttgtatct gttccagatg aaagattaaa caaactacaa gaattatata attctgaaaa
    14401 aatagttcct actgctatag aattttgtga tatagcaggt ttagttagag gagcttctaa
    14461 aggagaaggt ttaggaaata aattcttatc acatataaga gaagttgatg caatagttca
    14521 tgttgttaga tgttttgagg atgaaaatgt tgtacatgta gatggttctg tagacccact
    14581 tcgtgatata gaaactataa acttagaatt aattttctca gatatagaga ttcttgaaag
    14641 aagaattaat aaaactcaaa aagctgctaa ggctgataaa actttaggtt ctgaacttga
    14701 tttattaaaa tcaataatgt ctactttaga agaaagtaag tgtgtaagaa ctatggaatt
    14761 tacagaagat gaacaaacat tcgtaaattc attggattta cttacatcta agcctgtaat
    14821 atatgcttca aatgtatcag aagatgattt agctgataat ggagaaaata ataaatatgt
    14881 tcaacaagtt aaagcatttg cagaaacaga agatgccgaa gttgttgtag tatgtgcaca
    14941 aatagaagca gaaatttctg aacttgattc tgctgaagaa aagaaagagt ttttagaaac
    15001 attaggtctt gaacaatcag ggcttgataa gcttataaaa tcttcttatg ctttacttgg
    15061 tttaatttca ttcttaactg ctggtccaaa agaagttaga gcatggacta taaaagttgg
    15121 aagtaaagct cctcaggcag gaggaaaaat acattctgat atagaaagag gatttatcag
    15181 agcagaaaca atagccttta atgatttggt tgagcatggt actatggctg ctgctaaaga
    15241 aaaaggattg gttaaattag aaggtaaaga atacatagtt aaagatggag atgtaatatt
    15301 atttagattt aatgtttaaa atgattaatc gtatacactt tgactattat atataataac
    15361 taaaaaacta agattataat aatacaatat tatcttataa taattatata gaatttaaaa
    15421 ataaaagaag agtatttcaa atgatttgta taaattatta tgaaaaactc tttttttggt
    15481 ataataaaat tacttacata tataacattt aaatttataa attatctttt tattttttaa
    15541 aatatgtata acacacctat agaaattact attattacta tctaaagtat tttgctataa
    15601 tcccatatac tcttacctca aaaacattca aatgtaaaaa atactacata ttaataaact
    15661 ttgcccaata attcctaaaa taatatccta acaatgattt atgtttttat aatagtatat
    15721 ttttacataa aaaagtgaac attgattaac aaagttcact tttttataaa tttttatgtt
    15781 ataattcaca attttattta atttgtttct tttctattta aactatatct atctaatatt
    15841 aatttttcat attcttctat tggcattggt ctagcaaata aaaatccttg agccatatca
    15901 caacctataa accttaaaaa ctctgcttgc tctacagttt caacaccttc tgaaacaact
    15961 tttatatcaa gttgctttgc catttgaaca atacttgaaa ctacaatttg actctttttt
    16021 gcatttccca ctgttataaa gaattgccta tctaatttca atacatcaat tggcatatct
    16081 ttaagcatat ttaaagaaga ataaccagaa ccaaaatcat ccatagaaat tttaaatcca
    16141 acagatttca aagtttgcat tattttaaat aatatttttg tattgtcaaa tattgcactt
    16201 tctgtcaatt caatctcaat taaatttggt tcaatttcat attcttttgt tattctttca
    16261 agctcagaaa taaagcctgg attctcaaga tgtactcttg ataagtttat tgatatagga
    16321 aatgtgggga aattttcatt ttcccaacgt ttaaaatttt tacaaacttg ggtaaataca
    16381 aacatatcca attctgttat aaagccattt ttctcaaaaa taggaataaa tacacctgga
    16441 cttattagac cttttttagg atgttgccaa cgtacaagtg cttcagcacc tacaatttta
    16501 gtagttgata aatcaacctt aggctgtaaa taaactttaa attgttcatt ttttaaagca
    16561 tcatgcattt cttgttcaat atcacgttct tctatgagag tttcttttaa attttcagca
    16621 taaatctcat aaacttttaa atattttctt tttactgtag tttttgcaat catagctttg
    16681 tcaagacagg tagaaatatt tgtttctccc tcttcaacaa aatatacacc tatggatgga
    16741 attaaattga gttctactcc caaacttgtt tttatcattg acaatttttt acgtataagt
    16801 tcacatattt ctataattga ctctctatta tttttctttt caaatattat agcaaaattg
    16861 tcatttgaaa ttcttgaaaa tacagattgc tctttaaata tattcttaat tatctctgct
    16921 atttccttta atactctatc tccttcttca tacccaaacc tatcatttac tagcttaaac
    16981 ttatctatat caaaataaat taaaactaga ttctttttct catgagagga taaaaatttt
    17041 cccccttcaa gattaaattt atttgaattg ccaatacctg taacgttatc tttaaaagca
    17101 aattcttcca atttttcctt actcttttta tgtataaata gtatataaat tgttaatgcc
    17161 gtaaagatta taatcaagac tacacatgct gataatgtaa gttttataat agcatatgat
    17221 ttctcagata tagcagtttt aggaacttca gaaaaaagat accaatcatt gattcctatt
    17281 ggggcataac ttaaataata tccttttcca aacatactat aacttaatga acctgattta
    17341 aagtttttta tatttgactt catattattg atactagatt ttgatattga atcacttcta
    17401 ccaatgtaag aaaataaatt ttctttagta gagttggcgt ttttactgct tggatttaaa
    17461 acaatatcac cattacttct agttatataa gaatatcctt ttccattaaa tgtaggtaca
    17521 gaaagtattt tttcatacaa cttcgtacta tgtgtggcaa atataactcc tttaacctca
    17581 ttatctttat aaattggtac agaatataca ttaatattac tattggtatt tttatcatct
    17641 tctattacat cttttaattt gcctgttata accacttctc cttgcattga ttttaagaaa
    17701 taatctcttt tagagaagtc ttctatataa ccatctgtag tgtatgctgt accatttggt
    17761 aaaataactc ccattctttt aaatgaacta ttaatagctt gtctttttag tatagaaagg
    17821 atattatcta cttcaaaaga ctcttctcct tcaataaata ttgaaattga ctctagtaac
    17881 tttatatctc cattaatttg tttttttaac acattgacgc tttgatttgc tatttctgaa
    17941 agatattgat tcgtttcttc acctaatgtc ttattcaacc ttgaaccata aatcaaacag
    18001 gaaattgtaa ttactaagat acttatgaca gcaattatga acaaaccata cctattattt
    18061 tttgcatcac ttaacattat gcttcctgcc tttctcgaat ctatgtatgt ataatcatta
    18121 tcttatgaca attattgtaa acattttata ataatactta tttttccata tttactgtaa
    18181 ttatacaaaa ttataaactt tattacaaca ataaattaat taatatagat attagtgttt
    18241 atattattac attatctatt ttacaattat ttacatataa aaaaatgtaa tatcaaagtg
    18301 aagcttgata ttacattttt gttcttgtat tttgtttaat ttttatgcta catcttactt
    18361 aaacactcat ttgcaacttc tttatcatca aaatgatgct taactttacc tataatttga
    18421 taagtttcat gccccttacc tgttataatt acaacatcat ccttttttgc catacttata
    18481 gcttctgcta tagcttgttc tctatcaaca acaacatggt aattttcttt tttcttgtca
    18541 attccttcaa gtatatcttt tattattgcc tctggttctt cagttcttgg attatcagaa
    18601 gttataatac ataaatcaga atatttttgt ccaattgctc ccatcaaagg tctcttttca
    18661 gtatctctat ctccaccaca cccaaacaca gaaattattt ttccttctgc aaattgttga
    18721 gttgtcttta gtacattttc aagtgcgtca ggagtatgag catagtctac aattacgctt
    18781 atacctttat catttggaac tgtttcaaac cttcctgata ctccattaga aagtctaagg
    18841 ccttctttat atattggttt aggtatgcca agtacatagc aagcagctat aactgctaaa
    18901 gtattatata ctgtaaacat ccctggaact ggtataaata ttacttcttc atatgaaggt
    18961 gttattaatc tataagatac tccatccgaa tctgatttta tatctctagc cataaaatca
    19021 gccttagtat caacaccata agtgtaacaa ggtacattta taccttttat attttcatat
    19081 atcttttttc caccttcatc atctatgttt attatatttg ccatagtagt cttaaaaaat
    19141 aatttttctt ttgcttttct ataatcctct aaatctttat gaaaatctaa atggtctggt
    19201 gttaaatttg taaatatgcc tagtttaaag tctgtttcat caactctatt taatgccaat
    19261 gaatgtgatg aaacttccat tgcacagtag tcacatccat tatccaacat attattaaaa
    19321 tgatattgta aatctatact ttctggagtt gttgaattgg aaacaatgtc tttttctcca
    19381 tcaaaaattt ttatagttcc tataagtcca actttattct tatttaaagt taatatctca
    19441 tttagtatag tagttatact agtttttcca ttagtacctg taactcctat aacgttaaat
    19501 ttttgagatg gatgattgta aaaattatca gctacttttg ccatatcttt tcttgtatct
    19561 tttaccttta taaatgtaca tccttttatt gctacatctt cttcaacaag aaatgcactt
    19621 gctccctttt ctatggcatc ttttatatac ttatgaccat ctaacacaaa tccttttata
    19681 catataaata atgtaccttt tgtaaccttt cttgaatcat attgtacatt attaatatct
    19741 atatttaatt cacccttgac attgattata tccaacccct gtataatatc atttaatttc
    19801 ataaatgctt tcctcccttt aaactctttc tgctaaaatt acactacata taatattata
    19861 ttaagaattt tttactatgt gaatggttaa aaatgcttga aacattgcaa aaaatgctct
    19921 ataattttct ataatataac tgaggaaagg tcttctataa aaactatgga taataaaatc
    19981 gggtacttaa acgataactt taaaatattt aatattagag ataaaaaaga tatacaattt
    20041 gaataccata atcatgattt taataagata attatattta taaatggaaa tgtaacttat
    20101 tttgttgatg gtattcccta taaactaaag ccttgggata tactctttat cagcaataat
    20161 gaaatacata aaccagttat agattcaaat gtcttttatg agagaattgc aatatggatt
    20221 agcccagaat ttatgaaaaa aaatagtgat gctactagtg acttagaaaa atgttttaaa
    20281 atagctacag agaataaatg caatagattg cgtttaaata tacatgatat tgaatctatt
    20341 aagagtttta ttgagcaaat aaatatttcc gaatccaata ctgaatttgg aaatgaaata
    20401 ttgagaaatg cattatttat acaacttatg atattcttaa acagaatttt cctaaaaaat
    20461 gaaaatatgg aatcaacaga agacgttttt tatgatgaaa ctattaagaa agtattaaac
    20521 tatataaatt caaatataga tgataagctt tcaatagata gcatatcgtc tgaatttttt
    20581 ataagtaagt attatcttat gagaaaattt aaatctcata ctggaacgtc tatacacaat
    20641 tatatagttc aaaaaagatt aattttggca aagtcactaa taaccaatgg tcaccttatg
    20701 agtgaagttt gtaataaatg tggattcaac gattattcta gttttgtaag ggcatttaaa
    20761 aaaaactacg gtgtatctcc taaaaattat ataaacaata aaaatccttt agatattact
    20821 cttttagatg aatagataat ttatgtaaat tttattttaa taaaacttga agtattagtg
    20881 tatttatctt actaatacct caagtttttt aatgttttaa aatatatcaa aatataatga
    20941 tatatatttc tattacttaa aatatatatt aaataaattg catgttaact gaacctattt
    21001 atatatttat taaatctcac ataactttta gttacactat catatagcta tcctattttt
    21061 tcaatatcat ctcttaatat acttactcta ttctcctctt ttgctgtggt tactcctttt
    21121 ttaatgctta atttctgatt ttgttctaaa ttcgtaaata ctacaggact tattgttgat
    21181 agaccatttt tttcaatata ttctaagtcc attttaacta atttttctcc cgcttttact
    21241 tcatctccta cattcacaaa tacttcaaat ccatttcctt ctaagtttac agtttctatc
    21301 cctaaatgta taagtatttc tactccactt ttttgggatt ttattattat tgcatgctta
    21361 gttggaaata taactccaat agttccatca actggtgaat aaatcaatcc atcactagat
    21421 ttaattgcaa atccatcccc catcagttta cttgaaaata cagaatctgg cacttcttct
    21481 atatctaata tctctccatt tgttaatgaa tcaatctttt ctactaaagt attttccata
    21541 tttgcaactt ccatattttt tttcacttta tttttatcaa catttttagt aacagtactt
    21601 tcaacctttg tttttccctc tattatatct tttatatcag actttattga atctgatttt
    21661 ggtccaaata tagcttgaac attgttgcca ataaccataa cacctgctgc tccaagtgat
    21721 ttcaattttt ctttatctac tttatttata tcatttacaa ccaatctaag tcttgttata
    21781 cacgcatcta gactctttaa gttgtcttta tttcctaaag caatcaaaat ctctgatgct
    21841 aattcattct tagacatttc aatgacttca gcgttagttt ctgactcatc ttctcttcct
    21901 ggtgtcttta aattaaattt tcttatcata aatctaaata cactatagta taatactgca
    21961 aaaccaatac ctaccaagat agccaagtac catggagttc tattaggaag aacaccaaat
    22021 aataagaaat ctattaatcc tcctgaaaat gtgaatccaa ttttaactcc taataattgc
    22081 ataattgcgt atgataaacc tgaaaataaa caatgtattg caaataatac tggtgcaaca
    22141 aataaaaacg caaactctat tggctcggtt attcctgtta aaaaagaggt taatgctgct
    22201 gatgccatta tacctgctac aaattttttc ttttctggct ttgcttcatg caccatagca
    22261 agagctgctg ctggaagacc aaacatcata aatggaaatg cacctgtcat aaaagtacct
    22321 gctgtgaaat ttactccatc tttaagttga gcaaagaata tcttttggtc acccattaca
    22381 agctcaccag ctttatttac atattctcca aattgatacc aaaatggatt atggaaaaca
    22441 tgatgcaaac caaatggtat taatagtctt tccaaagaac cttccataaa tgctgctagt
    22501 gtttgatttg cttctataac attttttgag aaactaagta atccctcttg tattgtaggc
    22561 caaataaaag acaatataat tcctattgct atagcaacta atgaagtaac gattggtaca
    22621 aatcttttac ctgcaaaaaa acctagatac tgaggtaatt caattttata aaacttttta
    22681 taaagataag cagccactat ccctataata ataccaccaa atacccctgt ttgaattgtt
    22741 ggtattccaa caatatttgc atacatagaa ttgtctacca tatcttctgt aacacccgcc
    22801 ataacaccca tagatgtatt gagaattaaa aaacctacta tggctgccaa tgctgcaaca
    22861 ccttctcctt cagttaaacc aacagctacc ccaactgcaa atataagtgg taagttatta
    22921 aatataattt gacctgaact ttccattata cttattataa gttgaaatgt aggattcgcc
    22981 aaaactggaa aatgagccaa caatgcgtct cctgctaaag cattactcac gccaagtaat
    23041 atacctgctg caggaagtaa tgcaactggt agcattagag attttcctac tttttgtaaa
    23101 acaccaaata cttttttcat ttacttttct ctccttatta ttttatattt ttttaaataa
    23161 aaaagactta agtttataaa aagttacatt agtatactta attctaaaat taaacttatg
    23221 ttaatataaa taaacctata ttaacctaat ttcagaatta aaaataaact attataattt
    23281 ttttataaac ataagtcttg cctgcattat cagtaacaat cttaatgttt taaaattatt
    23341 attttatatt aaattttaaa taatatatta aaattaaaca taatatatta aatctataca
    23401 ttctgtatta aactactctt tattataaat tcagtacaga tataactctt ttgatatgta
    23461 atgttaaata aagtagttca tcttgactta catagtaatc ataattcttt tctacatgag
    23521 tttttatttt atatgcacaa tcataatcct tttcatactt tactttaatt atatctataa
    23581 tttcattatt tgtatcattg attttttctt tttttactaa tcgttttgca aagaatttta
    23641 aatgagtcag aagcctatca taatttatat catcttcttt aaattcaact ctataataat
    23701 atcttataat atttagtatg tcttttacaa ttttagtaat taatagtgat tcctttgaac
    23761 ttcccttata gtttgaattt actatatgca ttgctataaa accagcttca tctactggaa
    23821 atttcacatc aaattcttta tttatataat ctacagccca tttcccaatc tcatattctt
    23881 ttttatgtat tctttttatc tcatgtagta aatcattctt tatagttatg ttttcatgat
    23941 atcttttaat tgcaaatgca atatggtcag taagtgctac ataaatatat tcataaagct
    24001 tagtgttcaa attctcctct gcataagcaa ttatttcagt actaacctca aaaaatttct
    24061 catcaacctg gcttgctagt ttactaattt tatctaaaaa gctttcatct tgtattacaa
    24121 aagttctttc aattttactt ttatctattt cttgaccttt ctttttttga aacccaattc
    24181 cacatcctgt taatattaat tcttttttta tattttcatc taaaactaat acaacattat
    24241 tattataaat ttgttgaata atcataatca atatctctcc caaaatacaa tttcccttat
    24301 tcataaaaaa atacctaaat taagatacaa ttcacctgta taccttaacc taggtattgc
    24361 ctgcttacca gtaacaatcc agcttgttaa tgcttatata ttgtattcaa tttatggttt
    24421 tattctatta taaataaata tatatgtcaa gtaaattatt gaaaactatt tcctattttt
    24481 tagtattaat tttaacatca aaaatcaaat tttattccat tatatttatg attctatcat
    24541 catcatttca tcatagagaa tattttccaa taactcttta gccacttctc ttgatatttg
    24601 cttagtttca actaattgtt gaacactgtc tctttgactt tgtatcgcca ttactctgta
    24661 gtattctact cttgcctcaa acttatcatc attttgaatc tttctagtaa tccaattcaa
    24721 ataacgatta tagaatctga ttgctattaa ggcctcatgt ctgttatctt catcagtatt
    24781 tttatttata aatgatatag cagttttagt agaaagtgca tatgcatggt gaaactcttt
    24841 gattatttct ttattctcag atactatttt tgtaattttt tcttttactt ctttctttac
    24901 ttttctattt ataatccaca tgcttatttt taattttaca attttaaata tagatttttg
    24961 aacagatttt cttgattttg taatataaaa tctatacaat ttaaaggctc tatcagaaat
    25021 tttaccatct tctaatagtt tcaaaacagc taataatccc ttttggtctg cttctgcaat
    25081 catcatactt ataagtttat tatctggagt atttagtatt cctttttctg agtctaccaa
    25141 ctgttcattt aaatcatgta taactggttg tacttcttgt gctttttcat tactaagttt
    25201 taaaatggct tctttcaaaa ttagtttata tgcctcttta tcagataata aaggttctga
    25261 ctcttgtacg ttatttttta ataatcttgg cagtaaaact gttgccaata ctaaacttat
    25321 aagaataact tctgatgata taaataaaac agtatctctc attggaaata catctttatc
    25381 agccaaataa aaaggtatag ataaggcagt tgctagtgta aatgtaccat gaacaccaca
    25441 agtagcaact attagtgagt atttacattt agaaatactc tcttttacaa cttcttcatc
    25501 cttaaaacct aaaaaaccta caaaaaaatt atttaaagga ttctttttag gttttataaa
    25561 agaattatgc cataaatata cccatataaa tcttataaca aataaaataa gtacaattaa
    25621 agtagaaata aaagtcaatt ccaatgccat attttcattt ttactactaa gtcctgtata
    25681 tatacttggt aacaaaaaac caagcagtgt aaagacaaat ccattcaata catactccaa
    25741 aacataccaa gtattgtctg atattatcct taatttagta gttgtatttt gtaaatgctc
    25801 tttttcaatt ccatgagcta tccctgccat tacaacagct aggattccag atactcctat
    25861 ttcttctgct acaaaatata caaaaagtgg agtcattatt tgtataacta ttaccattgg
    25921 aatttcttcc aaattccact tgtgtaaact aagtcttatt tttataatta tgtatcctaa
    25981 aatacttcct actattattc ctccaaaagc tgtaatcata aattgaatcc ctgcctcttc
    26041 tattgaaaaa actcctgtaa ctgtggcaag tactgcaacc ttaaatgcta ctactccagc
    26101 agcatcattt aataaagatt caccttcaag tatactcatc atatttttag gaaaatttaa
    26161 tccttgtgta attgatttta ctgctactaa atcagttgga gatattactg ctgccaaagc
    26221 aaatgaaaca gataatggca tcccaggaat aagaaaatgt ataaagattc cacctgcaaa
    26281 cacggttatt aaaactaaac caaatgcaag aagcaaaatt ggttttctaa gctctaatag
    26341 ctcttttctc gatacatttt gtccttcact gaatagtaat ggagctatga tacatattac
    26401 aaatatttct ggattaaatg aaaatgagaa tttaaatggc aaaatagaca atataatccc
    26461 tagtacaatt tgatatattg caagaggaat attactccac tttttgctaa atacagttga
    26521 aatcgttgta caaaccaata atcccaacaa tgttactaaa attgacataa ctacctctct
    26581 tttcaataat aatattatag attaaactta tatatcaact aacttaatat taacagcatg
    26641 aaatcccatc ttaatcaaaa aaattttcag tttattctat tattcttatt atatacatca
    26701 aatttaattt ttgaaatatc tatactagat gacatgcaac aaaatgattt cttgatattt
    26761 cttttaaaac tggaatctct tttttgcata tttccttaca ataagggcat cttgtatgaa
    26821 acttacatcc acttggtgga tttgttggag atggtatgtc tccttttaat ataattctat
    26881 ttttcttaat tgtagggtct tgtattggta ctgcgctcat aagtgctttt gtatatggat
    26941 gttgagaatg taagtaaagt tctcctttag ttgcaatttc tattatagaa cctagataca
    27001 taacacaaac tctatgtgca atatgattta ctacactcaa atcatgagat ataaataaat
    27061 atgaaagttt atatttatcc tgtaaatcca taagtaaatt tataatttga gattgaatag
    27121 aaacatctag tgcagaaaca ggttcatcac aaactataaa ttctggattt aatgctaatg
    27181 ctcttgctat acctattctc tgtctttgac ctccagaaaa ctcacctggt tttctattta
    27241 aatggtactt agaaagacca caactttcta caacatttaa tatattatct tttatttcat
    27301 tctttggcac taaattatgt tctataactg cctcggatat tatttgttcc acatttaatc
    27361 ttggatttaa tgaactatat gggtcttgaa atatcatttg cattttgggt cttaactttc
    27421 taagttcttc cttctctaac ttatatatat ctacaccatt aaattcaact ttaccttcag
    27481 ttttttcaat taatctaaga atggttcttc ctgcagttga ttttccacat cccgattctc
    27541 caactatagc caaagtttgt ccttttttta aatcaaaaga aattccatct actgccttta
    27601 cacatttaat ttccttatca aataatccag atttaacttc aaagtacttt ttaagacctt
    27661 ctacttttaa taaactctct tcttttttca attatatctc ctccttttta tataaccaac
    27721 atgcaatact atgaccacta ttcaattcta ttaaaggagg tatctgagtt ctacatttat
    27781 cacatacttt ttcacatcta tcactaaaat aacatgaatc tggcattcca attggatttg
    27841 gtacttgtcc tggaatcgaa tacaatcttt tatctttgtt ttttccaaga gcaggctttg
    27901 attttagaag tccaatagta taaggatgtt ttggattttt aaataattca acaacctctg
    27961 ccacttctat aattttacca gcatacatca ctaatacttt gtcacacatt tctgcaacta
    28021 ctcccaaatc atgtgtaatc atcattatag atgtgtttaa tttttctttt atatttttca
    28081 ttatatctaa aatctgagct tgaatagtaa catctaaagc tgttgttggt tcatcagcta
    28141 taagtaattt tggattacat gataaagcca tagctatcat tattcgttgt ctcataccac
    28201 ctgataattc atgtggatat gaattatata tttcttcagc tcttggtatt tctaccagtt
    28261 ttatcatatc gattaccttt ttcttagctt cttcctttga taaatcttga tgtatcataa
    28321 taccttcaat gatttgactt ccaacagtaa acgctgggtt taaagatgtc attggttctt
    28381 gaaaaatcat tgatatctca ttccctctta ttcccataag ttcatcttca ttcatcttta
    28441 aaatgtcttt gcctcgaaag attatttctc caccttctat ttttgatgtt ggaagtaact
    28501 tcatcaaaga catggctgtt acagacttac cacatcctga ttctccaact attccaatag
    28561 tttcaccttc atttatatta aaacttactt catttacact ctttacaatc cctttatctg
    28621 tatgaaaata aacttttaaa tctttaacct caattagtgc tttactcatt attttctcct
    28681 ctcagcaaaa ttacttaaga taacatacac cttttgattg tattttataa aaatttaaat
    28741 tacaacattt tcttacattt tat
//
LOCUS       DAEMIK010000034        28441 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEMIK010000034 DAEMIK010000000
VERSION     DAEMIK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.034"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            580..1197
                     /locus_tag="KPK87_001287"
     CDS             580..1197
                     /locus_tag="KPK87_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HBG2537454.1"
                     /translation="MKGNEMENYKFVFDLDSTLTKQEILPEISKCINKYELMQNLTNE
                     TMLGNLSFEESFKKRVDLLKCIPISKVKSIVAKINLNEKIVKFIKENPDRCTVVTNNL
                     DIWICDLMKELSLENKYYSSIAHSNGDFIDKIKVIIKKEDIIKKMKGPIVAVGDGSND
                     IKMIENADIGIGFGGVRPIAPGILKVCDYAFYEEERLCQLLKQLL"
     gene            1170..1814
                     /locus_tag="KPK87_001288"
     CDS             1170..1814
                     /locus_tag="KPK87_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBG2537455.1"
                     /translation="MPTTKTIVVNCAGRGSRLGLNSNKSLIDIDGKPLIIRQLECLDD
                     YDDIRIVVGFQAEKLIEVVCSYRKDIMFVFNHDYLNTKTAASLSLGKKYSRDTVISLD
                     GDILFFKEDFDNFVNTDGEIIGVCQPYTKDPVYVKTDFINDGEFATEFSRTNGDYEWT
                     GLFKIYSKNLSSGTNHVYKMISPNLPLKIAHVELREIDTPEEYNEALKWYRNKK"
     gene            1827..2567
                     /locus_tag="KPK87_001289"
     CDS             1827..2567
                     /locus_tag="KPK87_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2537456.1"
                     /translation="MGNATRIYEKHVDIKKDTVQDFWNKRAKKHIPENPYISVKCNDQ
                     NPDYAKNLDKYEKEIIIPKLNITRNSNILDIGCGVGRLADDIVPICNYYLGTDFAEDL
                     ISIAKKRFSDNDCDFQACDFINTLYDTKVKSKMPFDTILLAGVTMYINDDELKIALKN
                     LLGILDKKATIYISIPIGINKRLTLKEFYSKELNSEYNVIYRTIEEYEEILKPLLDEG
                     FEIVESKSFLTDINQYSDTERHYFILKR"
     gene            2605..4080
                     /locus_tag="KPK87_001290"
     CDS             2605..4080
                     /locus_tag="KPK87_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HBG2537457.1"
                     /translation="MNYNKKNEVLNLIQILIEGIDYIKNNNLSESIVNSSLEAINYMK
                     KYINNSGRSNKNLLNLIDETYVKILRLSMYKNIRLIEDCELIISNLNYLSNIIENSLN
                     KKTKIVFMPYNAKMWNSLESIWKSAVLDEQCDCYVVPIPYYKLIDTPNGITQIYTYEG
                     NDFPEDVPVIHYDDFDLSKEKPDIIYVHNQYDDCNNATMVDSNYFSYNLKQYTNMLVY
                     VAYGILGTYPVSFYLNFYELIASRNFDKVIVQSPAFEIIAECSGINKNQILTMGSPKF
                     DSLIYNLKQKNINKNYESKLKGKIIFLWTTNLMKIPNGKDGVIDEIENVFDIIENSQE
                     YGLIYRPHPLELEYVKSKVPECFNRYKTLLDSISIKNNIILDDSVSYYESFNLSHALI
                     TDRSSVLIEYIQTKKPILIYDIDMERGYYDSRIFDIFSNYVVGEEDMDLIKFMNLVKN
                     NNDYKLNQRLNSLNSVLSNTDGSCGEKIHTNVLEYVLNNHI"
     regulatory      4606..4696
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5101..5418
                     /gene="flgB"
                     /locus_tag="KPK87_001291"
     CDS             5101..5418
                     /gene="flgB"
                     /locus_tag="KPK87_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG2537458.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            5426..5830
                     /gene="flgC"
                     /locus_tag="KPK87_001292"
     CDS             5426..5830
                     /gene="flgC"
                     /locus_tag="KPK87_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG2537459.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            5840..6157
                     /gene="fliE"
                     /locus_tag="KPK87_001293"
     CDS             5840..6157
                     /gene="fliE"
                     /locus_tag="KPK87_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG2537460.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            6182..7732
                     /gene="fliF"
                     /locus_tag="KPK87_001294"
     CDS             6182..7732
                     /gene="fliF"
                     /locus_tag="KPK87_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG2537461.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            7745..8815
                     /gene="fliG"
                     /locus_tag="KPK87_001295"
     CDS             7745..8815
                     /gene="fliG"
                     /locus_tag="KPK87_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG2537462.1"
                     /translation="MSTEITNVNAFDLSDISESDKNEKIEGEGLKKAAVLLMTLGPDV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAIRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDAISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIVNS
                     VRKLEREGAITIVRNFGDDFVE"
     gene            8799..9539
                     /locus_tag="KPK87_001296"
     CDS             8799..9539
                     /locus_tag="KPK87_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG2537463.1"
                     /translation="MTLLNNKIIKSKKASYKGVLDLSIIDKVRVGESNLNKENDCRFR
                     DEVIKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFKLFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            9543..10862
                     /gene="fliI"
                     /locus_tag="KPK87_001297"
     CDS             9543..10862
                     /gene="fliI"
                     /locus_tag="KPK87_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG2537464.1"
                     /translation="MYKTDIYIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            10893..11339
                     /gene="fliJ"
                     /locus_tag="KPK87_001298"
     CDS             10893..11339
                     /gene="fliJ"
                     /locus_tag="KPK87_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG2537465.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFDKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            11368..12569
                     /locus_tag="KPK87_001299"
                     /pseudo
     CDS             11368..12569
                     /locus_tag="KPK87_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
     gene            12587..13195
                     /locus_tag="KPK87_001300"
     CDS             12587..13195
                     /locus_tag="KPK87_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG2537466.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEQESPEEA"
     gene            13222..14205
                     /locus_tag="KPK87_001301"
     CDS             13222..14205
                     /locus_tag="KPK87_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG2537467.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQIYAFQNPAGLDAAGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            14218..14421
                     /locus_tag="KPK87_001302"
     CDS             14218..14421
                     /locus_tag="KPK87_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG2537468.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            14421..15239
                     /locus_tag="KPK87_001303"
     CDS             14421..15239
                     /locus_tag="KPK87_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG2537469.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            15229..15924
                     /locus_tag="KPK87_001304"
     CDS             15229..15924
                     /locus_tag="KPK87_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2537470.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            15936..16379
                     /locus_tag="KPK87_001305"
     CDS             15936..16379
                     /locus_tag="KPK87_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG2537471.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            16394..16768
                     /locus_tag="KPK87_001306"
     CDS             16394..16768
                     /locus_tag="KPK87_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG2537472.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            16769..17434
                     /gene="fliP"
                     /locus_tag="KPK87_001307"
     CDS             16769..17434
                     /gene="fliP"
                     /locus_tag="KPK87_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG2537473.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGSQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            17447..17716
                     /gene="fliQ"
                     /locus_tag="KPK87_001308"
     CDS             17447..17716
                     /gene="fliQ"
                     /locus_tag="KPK87_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG2537474.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            17728..19542
                     /locus_tag="KPK87_001309"
     CDS             17728..19542
                     /locus_tag="KPK87_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG2537475.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            19551..21626
                     /gene="flhA"
                     /locus_tag="KPK87_001310"
     CDS             19551..21626
                     /gene="flhA"
                     /locus_tag="KPK87_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG2537476.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELISNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            21601..22617
                     /gene="flhF"
                     /locus_tag="KPK87_001311"
     CDS             21601..22617
                     /gene="flhF"
                     /locus_tag="KPK87_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG2537477.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            22614..23492
                     /locus_tag="KPK87_001312"
     CDS             22614..23492
                     /locus_tag="KPK87_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG2537478.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            23506..24207
                     /locus_tag="KPK87_001313"
     CDS             23506..24207
                     /locus_tag="KPK87_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG2537479.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            24223..24645
                     /locus_tag="KPK87_001314"
     CDS             24223..24645
                     /locus_tag="KPK87_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG2537480.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            24630..24815
                     /locus_tag="KPK87_001315"
     CDS             24630..24815
                     /locus_tag="KPK87_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG2537481.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            24856..25629
                     /gene="flgG"
                     /locus_tag="KPK87_001316"
     CDS             24856..25629
                     /gene="flgG"
                     /locus_tag="KPK87_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG2537482.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            25650..26411
                     /gene="flgG"
                     /locus_tag="KPK87_001317"
     CDS             25650..26411
                     /gene="flgG"
                     /locus_tag="KPK87_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG2537483.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            26434..27315
                     /locus_tag="KPK87_001318"
     CDS             26434..27315
                     /locus_tag="KPK87_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2537484.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            27318..27692
                     /locus_tag="KPK87_001319"
     CDS             27318..27692
                     /locus_tag="KPK87_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2537485.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            27724..>28441
                     /locus_tag="KPK87_001320"
     CDS             27724..>28441
                     /locus_tag="KPK87_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537486.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNK"
BASE COUNT        11721 a         2999 c         5144 g         8577 t
ORIGIN      
        1 tcgatattgg catcaaataa aatagaatat actcaatgat tttagtatat agaagattta
       61 attcaaggga aactaatttt atcatattta cttataataa taaaatagat aagcatgttc
      121 aaaaaataaa caacaaaaaa caatataaat gaaaattata catattttct cttaattttt
      181 tttaaaatta gacgataata tgtatataat taaatcgggc aatatcctaa aaacgaaggt
      241 tttgcacatt aagacaattt tttagtgatt tcaatgattt tagtgttttt aaataattga
      301 attaattcga cataaaacag cagtatttgt aataattgaa tttttattaa gtcattttaa
      361 tacttaaaaa ccatttatag agtatttttg ttagttaaga acgcaagatt tctttatttt
      421 gataagattt agtttttagc tttttgattg agctatatac aattttataa taaaaaataa
      481 aattatatta taaaattata aactctgtaa aattattgaa atttctaact caatattgtt
      541 tgatgtactt atcactcaaa aataggattt tatgcttcaa tgaaagggaa tgaaatggaa
      601 aattataaat ttgtttttga tttagattca acattaacta aacaagaaat acttcctgag
      661 atttctaagt gtataaataa atatgaactt atgcaaaatc ttacaaatga aactatgcta
      721 ggaaatttat cttttgagga aagttttaag aaaagagtcg atttattaaa atgtattcct
      781 atttctaaag ttaaaagtat agttgctaag attaatctta atgaaaaaat tgtaaagttt
      841 ataaaagaaa atccagatag atgtactgtt gtgacaaata atttagatat ttggatttgt
      901 gatttaatga aagagctttc tttagaaaat aaatactatt catctatagc tcattcaaat
      961 ggtgatttca ttgataaaat taaagttata ataaaaaaag aagatattat aaaaaaaatg
     1021 aaaggtccta tagttgcagt tggtgatggc agtaatgata taaaaatgat agagaatgct
     1081 gatattggaa ttggatttgg aggggtaaga cctatagctc ctggaatatt aaaagtatgt
     1141 gattatgcat tttatgagga ggaacgttta tgccaactac taaaacaatt gttgtaaact
     1201 gtgcaggtag aggaagtaga ctaggattaa attcaaataa gtcgttaata gatatagatg
     1261 gaaagccatt aataataaga caattagagt gtcttgatga ttatgatgac atacgtattg
     1321 tagtaggctt tcaagcagaa aaattaattg aagttgtatg ttcttatcgt aaagatataa
     1381 tgtttgtatt taatcatgat tacttaaaca ctaagactgc tgcaagctta tctttaggca
     1441 aaaaatattc tagagatact gttatatcac tagatggtga tatacttttt ttcaaagaag
     1501 atttcgataa ttttgttaac acagatggag aaattattgg tgtatgtcaa ccgtatacta
     1561 aagaccctgt ctatgttaaa acagacttta ttaatgatgg agagtttgct acagaatttt
     1621 caaggacaaa tggagattat gagtggacag gtttgtttaa aatatattct aaaaatttaa
     1681 gctctggtac aaatcatgta tataaaatga tttcacccaa cttaccactt aaaatagctc
     1741 atgttgaatt gagggaaatt gatacaccgg aagaatataa tgaagcttta aaatggtata
     1801 ggaacaaaaa ataaaggaga ttttatatgg gtaatgcaac gagaatttat gaaaaacatg
     1861 tagatataaa aaaagatact gttcaagatt tttggaataa acgagctaaa aagcatattc
     1921 cagagaatcc ctatatttca gtgaaatgta atgaccaaaa tcctgattat gctaaaaatt
     1981 tagataaata tgaaaaagaa ataataattc ctaaactaaa tataactaga aattcaaata
     2041 tattggatat aggttgtggg gtaggtaggt tagctgatga tatagttcct atatgtaact
     2101 attatcttgg gacagatttt gctgaagact taatatctat tgctaaaaaa agattttctg
     2161 ataatgactg tgattttcaa gcatgtgatt ttatcaatac tctatatgat acaaaagtta
     2221 aatccaaaat gccatttgat acaattctat tggcaggtgt aacaatgtat ataaatgatg
     2281 atgaattaaa aatagcattg aaaaatttat taggaatatt agataaaaag gcaacaatat
     2341 atatatctat acctattgga ataaataaga gattaacttt gaaagaattt tactcgaaag
     2401 aattaaattc agaatataat gttatatata gaactataga agaatatgaa gaaattttaa
     2461 aaccattact agatgaagga tttgaaattg ttgaaagcaa aagcttctta acagatataa
     2521 atcaatactc tgatacagaa aggcattatt ttatactaaa gcgatagttt taatataaat
     2581 aaaatcaaaa ttaaggtgaa tctgatgaac tataataaaa aaaatgaagt tttaaatctt
     2641 atacaaatac ttatagaggg tattgattac ataaaaaata ataatttaag tgaatcaata
     2701 gttaattcat ctcttgaagc tattaattat atgaaaaaat atataaataa ttctggaaga
     2761 agcaataaaa acttgttgaa tttaattgat gaaacttatg taaaaatttt aagactttcc
     2821 atgtataaaa atataagact tattgaagat tgtgaactta tcataagtaa tttaaactat
     2881 ttatcaaata taatagaaaa tagtctaaat aaaaaaacta aaattgtatt tatgccttat
     2941 aatgctaaaa tgtggaatag tttagaaagt atctggaaat ctgcggtttt ggatgagcag
     3001 tgcgactgtt atgtagttcc tataccttat tacaagttaa ttgatacgcc taatggtata
     3061 actcagatat atacatatga aggtaatgat tttcctgaag atgttccagt aatacattat
     3121 gatgattttg atttatctaa ggagaaacct gatataatat atgttcataa tcaatatgat
     3181 gattgtaaca atgctacaat ggtagatagt aattattttt cttataactt gaaacaatat
     3241 actaatatgc ttgtatatgt tgcttatgga atacttggaa cttatcctgt tagtttttac
     3301 ttaaattttt atgaattgat agcatctaga aattttgata aggttattgt gcaatctcct
     3361 gcatttgaaa ttatagcaga atgtagtggt ataaataaaa atcaaatact aacaatggga
     3421 tctccaaaat ttgattcttt aatttataat ttaaaacaga agaatataaa taaaaattat
     3481 gaatctaaat taaagggtaa aataattttc ttatggacaa ctaatctaat gaaaatacct
     3541 aatggcaaag atggtgtcat tgatgagatt gaaaatgtat ttgatattat tgaaaatagt
     3601 caagagtatg gtcttatata tagaccacat ccattagaac ttgagtatgt aaaatctaaa
     3661 gttccagaat gttttaatag atacaaaact ttgctagatt caatatctat aaaaaataat
     3721 attattttag atgactctgt atcttactat gaatcattca acttatccca tgctttaata
     3781 acagataggt cttctgtatt aatagaatat attcaaacga agaaacctat attaatttat
     3841 gatatagata tggaacgtgg atattatgat tcaagaatat tcgatatatt ttcaaattat
     3901 gtagtaggag aagaagatat ggatttaata aaatttatga acttagtaaa aaataataat
     3961 gattataagc taaatcaaag attaaattct ttaaatagtg tactatcaaa tacagatgga
     4021 agttgtggtg aaaaaataca tactaatgta ttagaatacg ttttaaataa ccatatttag
     4081 ttaaaattaa ttaataatta gataaattta ggcttggaaa ggagttaaaa cattatcaat
     4141 tttaataaat ataagataat aaagggcaaa ttaattaaag tttgaccttt tttaaattta
     4201 ttttgttact ctatacaaca ttttagactg tattcttata taaccagtct aaaataaaat
     4261 ttatcaaaag tagagaaaat tacaaatttt ttatccctaa aagtgaaaat aaaagggaaa
     4321 actttaaaca gatataaaat cctaagcata taattggaaa ataatgttaa aataaatgct
     4381 tttaatgaaa ttattgtaaa agacaaattt agaataaatt tattttatat tgtaaatttt
     4441 tgtaaatttt taatatatat gtacatcata taaattaaat gttataaata taaatatatt
     4501 aaaaatatta taaaatttaa tattagaaat taaattaaaa aattatataa aaaaatactt
     4561 aaaaaaatat ttaaaaaatt tgttatttat tgtatactta agttaaaact aaataggcaa
     4621 atctagagaa atctagtgac gcaaagctat agggactaag gtttatatac ataaactatg
     4681 tcagccagtt gccaaaaaga gtcctaggtg tattgtatac ctaagaaaag tctataatga
     4741 ctgccttttt ggcagtcatt ttgttatgga aaaatcactt tatttcagta gaaaatacat
     4801 ataataacat attttaccaa ttataacata aaataaagaa aaattatctc aaaaagttac
     4861 tattttacaa aaatgacttc tttatttatt taaattaatc gcatttccat tacattaata
     4921 caataatgta tcaatttagt aagaaaacta atattttttt aatgaagtcc catgaaaata
     4981 taaaaaaatt ttaatatgta tttaaatatt tcttcgaaaa gttgctcttt ttgtaatata
     5041 gcaacttcat tttttattaa taaacaaaat agataaaata tattttgagg aggggtgagt
     5101 gtgaatacat atgacattat aaaaatggga ttagatgcaa ctaatctaag aagtcagaca
     5161 atagcaaata atatagcaaa tataaatact gaaggatata aaagacatta tataactttt
     5221 gaagaaacac taaatgatgc tatgcctaaa atagaagaaa aaacagacaa ttctaaaggt
     5281 ttaagagtag atggtaataa tgtagacata gaagaagaaa aagtaaatca agcaatggca
     5341 acattacaat ataatgcttt gataagtttt gcaaatggaa aaatagcaat gaccaaatca
     5401 ataataagtg ggaggtaatt agaagatgag tgtgttcagt ggaatgagaa tatctgctag
     5461 tggattaaca gcagaaagtt tgaaaatgga tatcatatca tccaatgtag ctaatataaa
     5521 tgctacaaga ggtccaaatg gagaagcata taggagaaaa gttgtttcat ttgaagaaaa
     5581 ctatgataat aaattaggta tgctaggagt aaaagttaca tctgttgaag atgataaatc
     5641 acctcttaaa gaagtatatg acccatctca tccagatgca aagccaaatg gatatgtaga
     5701 atatccaaat gtaaatatat tggtagaaat gacagattta ataacagctt cacgagcata
     5761 tgattctaac gtagatacat tgaatgctca gaagtctatg atatcaaaag ctttggaaat
     5821 aggaaagtag ggataaatta tgccaattac acaaattaac aatttaatgc aaggtgcaaa
     5881 gttagataat ttgattggag aagcaaagcc attaactctt aataataaag aagaaaaaaa
     5941 gaattttgaa gatgtaataa tagatactat agataaagtt aattttcaac aagtaacagc
     6001 agatgatatg aaagaaaaat ttataaaagg cgaagatgtt tctatgcatg aagttatgtt
     6061 aataggtcaa gaagcacaac tatctatgca atatttaata gaagttagaa ataagattca
     6121 tgatgcatat caagaaatga ataaggtgca aatttagcct tttgaacagg agtaataatc
     6181 aatgataacc agttttaaaa ataagattaa ggagcaagtt gctaaatcta ggacaaagat
     6241 aagttcttta aaaaagggaa ctaaaatagc attaggtata ggggttattg ctatatgttt
     6301 agcgctagta tttacagcaa tatctgcgag caaaaataag tatgcagtct tattttcaga
     6361 actagatgct caagatgctg cagatatatc agcagagcta gagtctaaaa agatagatac
     6421 taaggtaaaa ggaagtacga tttatgtacc tagaacagaa gttgataagt taaggctgga
     6481 gttagcgcca agtcttacag gtggttctaa aggatttgca ctaatggatg atagttcatc
     6541 aatagggctt actgatgaag aatttcagat taagaaacaa agaatgatac aaggtgaaat
     6601 agaaaaaaca ataaagagtt ttccacaagt ggaagacact agagtacata taacatttgg
     6661 agagtcatca gtatttgaga aggaggctgt tccagggagt gcagcagttt atttgatgct
     6721 aaaaccaggg acgactttag aaaaaaacca aataaagtct gttatggcac ttatatcagg
     6781 aagcgttaca aatataccag agaaaaatgt tcaggttatt gataacaaaa tgaatttatt
     6841 atctgaaggt attttggagg atgaaaatgt tgatgaaaat aatcaaccaa taaatagtgg
     6901 cattagtgtt gataaaaata aggcatctga acaagcattg aataaacaac tagaaaagtc
     6961 tattttagaa atgctagaac cgatatttgg taagggaaag gtgaaagcta cagtaaatgc
     7021 tgatttaagt tttgatacag ctgaaaaaac tgagataaaa attgaccctg ataaagttgc
     7081 aataaaggag acaaggtcga aaaattcatc taaatctgct gcaaataatg ttcaaggtgt
     7141 agatgctaac atgaataacc aaggaaataa caatggtact aatacagaag atagtttaga
     7201 tgagagtttt gagtatgaaa caggtaagat tgaaactcac actatagttg cgcctggaga
     7261 acttaataga ttgacagctt ctgttgcaat agatggaaaa atagctaaag gtgttcaagc
     7321 tgatgtagag gatattgtta ataatgctat aggtatggat ggagcgaggg gagatactct
     7381 tagcgttgtt tcaatggtat ttgacccaga aggtaagaaa gaggctaaac aagagctgga
     7441 agagattaaa aaagaaggat ttaaaaagaa cataataaaa gcagttattg gagtagtagc
     7501 agtaatagca gttggagcta ttggatactt aatatataag aaaagaagtg aacgagaaga
     7561 tatggattat gaagatgaag attatatttc caaatctgat tctgttgata ggaaagataa
     7621 tcctctatta ggagatgacc cagaactgac attagaggaa gctgttaaaa tgtatgcaga
     7681 agaaaagcca gaccaagtaa cagagataat aaaaacttgg ttaaatgagt aagggggata
     7741 gaaagtgtcc acagaaatta caaatgttaa tgcgtttgac ttaagtgata taagtgaaag
     7801 tgataagaac gagaaaatag aaggtgaagg tttaaaaaaa gctgctgtat tattaatgac
     7861 attagggcca gatgtctcta gcgatataat aaaaagtttg cctgataaac aagttcaaaa
     7921 aataggtttg gaaattgcaa atatttcttc aatagcttct aaagaaagaa gagaaatctt
     7981 aaaagagttt gtagaaataa atagagcaaa agattatgtt atagaaggtg ggcttgaata
     8041 tgcaagagag ttattaagta atgctttggg aaatgctaaa gcaaacaaat tacttgaagg
     8101 tatatcaata aatacaagta caaagccttt taaacatatt atcaaactag atgtaaagaa
     8161 gatattagaa atacttagga atgaaagtgc acaaactata gccgttttac ttgcacatat
     8221 ggaaccagaa aatggggcag aagttttatc atatttagac tttgatttac aaaatgaagt
     8281 agctattaga ataagttcaa tgggttcaat atctccagaa gtaataaagg taattgaaag
     8341 ttcattagaa gataaattac attcaataag tggtgatgat tttaaggatt atggaggagt
     8401 gtctacctta gttcaaatat tgattaattc tgatagaaaa acagaaaaga atatcttggg
     8461 aactattgaa gaacaaaacc cagatttagc agctcaaatt aaatctcata tatttgtctt
     8521 tgaagatatt gtcaagttag atgatataag catacaaaaa gtgttaaaag aagtaactat
     8581 gaaagattta gtatatgcct taaaaggtgt ggatgatgcg atttcagatg ctatatatag
     8641 aaatcaatct tctagggcag cagaggcact tagagaagaa atggaaatgg ttggtgttat
     8701 aaaggttaca caggttgagg ctgctcaaca aaaaatagta aatagtgtaa gaaaacttga
     8761 aagagaaggt gccataacta ttgtaaggaa ttttggtgat gactttgttg aataataaga
     8821 ttattaaaag taaaaaagca tcatataaag gagttttaga tttatccata atagataaag
     8881 ttagagtggg agaatcaaat ctaaataaag aaaatgattg tagatttagg gatgaggtaa
     8941 taaaggaagc tatagaatct gcagagataa aaaagaatga gatattagaa gaagcaaaaa
     9001 taaatgcaca aaatatagaa aagcaagcat atgaaaaagg gtatagtcaa ggtcaaaaaa
     9061 atggatatga ggatggatat aaagagtcat atgatgaata tacccaaaaa gctaaagatg
     9121 aagcagattt tataaaaaat caggcaaatt taatcttatt acaagctaat gagaagatga
     9181 ctgagtacat aaaagaaaaa aataaagaga ttgtaagact tgctattaat atggccagtt
     9241 ctgttttaaa aaagcatttc gaagatgaag aatctatgag tgatttgtta aagcaaatta
     9301 ttcaagatta tgaaggaagt agttcattta tagtaagatg taattctttc tataaagata
     9361 atatagagaa agaatttaaa ttatttaaag aggaaacatc actaaaatca agtgtatttg
     9421 ttattgcaga tgatagtata gataaaggaa atgctattat ccaaaaggaa aatggaagaa
     9481 taatagttgg tattgattgt gctatggaaa aactaaaaga agaattgctt ggtgaataaa
     9541 gtatgtataa aactgatata tatatagatt ttgacaaaat aaataagaaa atagaaaatt
     9601 tagaactcac tatatcagaa ggtagagtaa agaagataat aggacttact gtagaagttg
     9661 aaggaataaa agcttttgtt ggggagttat gcgttatata caaccaagtt aataaaccag
     9721 tgaattgtga agtagtaggt tttaaagata aagaagtcat attaatggcg ttgggagaat
     9781 taacattgat agcacctggt tgtaaagtta tttctaaggg gataccttta agtgttatgt
     9841 gtagtgataa tttactaggt aaagtacttg atggtcttgg aaatcctata gacaactcag
     9901 atgctgtttt aggagataga tacaacctga acaatgaacc tcctgaccca atgaagagaa
     9961 aaaagatacg aaatataatg gaaacaggtg taagagcaat agatgcattt actacttgtg
    10021 gtgaaggaca gagaatcggc atatttgcag gaagtggagt aggtaagagt acgactctag
    10081 gtatgattgc taggaatgca aaagcagatg taaatgtaat tgctcttatt ggagaaagag
    10141 gaagagaagt actagatttt attgataaag atttaggtga agagggaatg aaaaaatctg
    10201 ttgttgtgtg tgcaacatca gataaagccc ctctagttag gttaaaaggt gctcttactg
    10261 ctactgcaat agcagaatat tttagagacc aaggcaaaaa agtaatactt atgatggatt
    10321 cagtaactag atttgctatg gcacaaagag aagttggtgt agcaatagga gagccaccag
    10381 ctcaaaaagg atatacacca tctgtatttg caatgttgcc aaaacttatg gagagaacag
    10441 ggacatcaga taaaggttct ataactgcat tttatacagt attagttgat ggagatgact
    10501 ttaatgagcc aatagcagat acaacaagag ggatacttga tggtcatata gttttatcta
    10561 gagacttggc aaataagaat cactatcctt ctatagatgt attaaacagt ttaagtaggc
    10621 ttatgaatga aattgcttca aaagaagaca taaaaatagc gtcttttgca agagacatgc
    10681 ttgctgagta tagagaagca gaagatttga taaatatagg tgcatatgct tcaggtacaa
    10741 ataaaaaaat agatgaagca atctattatc atgagcatat tattaatttt ttaaaacaag
    10801 ggataaatga aaagagttct tttaatgaaa ctataagtag tttgagaaga gtttttgaat
    10861 aaacaattaa aaaaatattt gaataggttg tgatggaatt ggataaaaac tttaagttta
    10921 gattacagaa agttttagac ttaaaagtga aagatgaaga agagataaaa atggaatttg
    10981 ataagattca acaaaaaaaa atagatatag agactaattt agaaaactta gaaagtaatt
    11041 atagcaaata ttctataagt aaaaataacg atagtattca aaatcaaaaa ataactatta
    11101 actatttact agctttaaac aattctataa tggatttatc agaagagttg gataagtcaa
    11161 caaatgaact tgaaaaagct agaaagcagc ttataagcaa acagatagag agaaaatcat
    11221 tagaaaaatt aaaagaaaaa aagtatggac agtattataa agaagaaaat ttaaaagaac
    11281 aaaacactaa tgatgagttt gcaagtatga gttatttaag gaatagacaa gttctataag
    11341 aatagtagga agtgaggata ttattgtatg aagatacaag cagattctta taatattatt
    11401 aaagatattg gggatagaaa aagttcatct aagaacaaaa atataaaaaa taataaattt
    11461 gaagataatc tatataaggc aacagaccat aaaaaggtta aaaatagaag ggaagtaagc
    11521 aaaatattag ctcagataaa ccggaaaata gtgataatga gtgtcattta gatgaagata
    11581 aaagtaatca cttagaagat gaaaatacat atgaaaaaga ttgtagtaag aataatgaag
    11641 ctttgcagca attactgaat cttttaaagg ataattataa aaaagataaa gatataaaca
    11701 ctatattgaa taatatagaa aaactaagta tatcagatga attaaaacaa gaacttaagc
    11761 ttaataaaaa tatattaaat aagattgcaa atcaaaattc agagctaaat aaagctaata
    11821 taaataactc taatgatata gaaaatttag acatagttaa attaagagaa cttttggaag
    11881 ataatgctaa taaagatatt gaagtaatta ctaaaactac agaaaatgat actaaaaata
    11941 tagatttgta tagttttgat agcaatagaa tggataatct taactcaaat aaacagaaag
    12001 atgatagctc tgatattttg gaaaaactag caggggtaaa tggaaataag agtgggaatt
    12061 ttggacaagt aataaataaa aatgttgata tgaataaaat taaaaatgat aatgtcaatt
    12121 taaaacagat agaatcaaat gtaatggatg actctataaa agctattaag catatgaaaa
    12181 caaatgatat acaagaattg acaattaaat taagaccaaa agaattagga gatatgaata
    12241 tacaattgtt aaaagatggt gaaagtatga gagctgttgt gactgttttc aataaagatg
    12301 tctttgactc tataaataaa aacatagcag atttaaagca acatcttgaa cttactaatg
    12361 taaatataaa agatgtatca gtgcaaatgc acagcgataa tacaaataca tctgatacct
    12421 ttgataaagc atttgaacaa cagaatagac aaaataacca agaaaacatg agtaagaaga
    12481 atacaaatga taagcataat aatgtagtca tagaagatga cattaaagaa aatattattg
    12541 atgatgatag agtagattta ctagcttagt aagggggaag tattgcatgt acggaatagc
    12601 aacggctttg gcaggaactc agggaaacta taggtcaaat aacttaagcc aacttgaaag
    12661 aaatattaat gatattgcca ataaacaagt tagaagcaaa ggaaatcaga cagtaacaga
    12721 caatgggact ccaattatag gacaaggtga tgatgctgat aaagacttgt ttatgaagat
    12781 acttgtggca cagatgggta atcaagaccc aatgaatccg caagacccaa cagaatatgt
    12841 gtcacaatta gcacagtttg caactttaga aaagatgtca gctatgaatg agaagctaga
    12901 tgtactttta gccttaagta attccactct aataaactct gctttgagta tggcaacatc
    12961 attaattggt aaagaagtag aagtatctgg ggaagagaaa gaagatgaaa acattgtagg
    13021 agtagtaaag tccacatata taaaagatgg ggaagtacac ttagaattag aagtagatgg
    13081 aagtgacaaa actgtaggtg ttaaatatga aaaattactt aaaataggag aggtagatga
    13141 aaattcagga gaagaaaatg actcttcaga acaagaatct ccagaagaag cataatatta
    13201 aatgagaaaa gaggtaaatt catgataaaa gcaatgtatt caggtgtaag tggaatgaaa
    13261 gcaaatcaaa caaaattaga tgtaataggt aacaatgtag ccaatgctgg aactacttct
    13321 tttaagaaaa gttcagctag aataacagat tcattttatc aaactgtact atatgcaagt
    13381 gctccaacag ctgcattagg aggaacaaac ttaggacaag taggtgtagg tagtaagata
    13441 tcaagtataa ataaagatat ggtacaagga aatgtacaac caactaatag accatctgat
    13501 ttaatgatag atggagatgg atatcttcca gtagtaagac aaggaacagt tatgtatacg
    13561 agagatggtt cttttaattt agatacagga tttaaagcag ctgatggtgc tttgaagaat
    13621 attaaaggtg gaagacttgt aaatggtgat ggatatatgt tacaaggtat gatttatgat
    13681 ggggaatata aagctgatac tggagaattt gttaaaaaag gtgatgctaa aggaaaagag
    13741 cctattgtaa taccacttca aagtataagt cctaaggatg gaacagttca aaatgtacta
    13801 gagtataata tatcaaagga tggaacagta gaatttttat tgagtgatgg tcaaagaact
    13861 caatttgtct atgatatacc tgatggtgct aaggtaggag atacacctgc tggaatagat
    13921 aaattaaaag gtacaataca aaaagtgcaa atatatgcat tccaaaatcc agcaggcctg
    13981 gatgctgctg gaggaaactt gtttaaacca tcagcaaact caggagaccc acaaatagca
    14041 ggagctacag gaacaataat acaaggggca atagaagctt ccaatgtaga tatagcagaa
    14101 gaatttacag aaatgataat agcaagtagg tctttccaag caaacagtaa aataatatcg
    14161 acatcagacg agatacttca agaaataata aatcttaaga gatagtaggt acatattatg
    14221 ataagagtaa cggatttaaa cggaagaaaa tacatattaa attcagacct tatagtgagg
    14281 attgattctg taccagaaag taggattctc cttacaacag gagaaaaaaa ccttgtaaaa
    14341 gagtcggtag atgagatagt agataaagta ataaagtata aaagaaaaat aaatgtaggt
    14401 tatattagga gcgaaaaata atgaaaaaaa cagattcact gacatttatt ggactaatag
    14461 tatctacagt attagtttta gttggtgccg caaaaggaag tagttcaggg ttaaagaatt
    14521 tttttgatgt ttcatcaata ttaatcacag tgcttggttc atttggagct ctaatgatta
    14581 catttactat agatgatata aaacttataa aaaatgcatt acaatactca tttaagacaa
    14641 tgagtgtatc aaagttagat ttactagaac aatttaaaac actttctaaa aaagctagaa
    14701 aagaaggtct tttatcaata gagagtgatg taatgggaat agaagatcca tttatgaaga
    14761 aagggttaga gctgtgtatt gatggattgg aaatcaatga gataaggtcc atcttagaac
    14821 tagagataga ttctgttgaa gatacaaata acagatactc taaactattt aaaacttggg
    14881 gaacctttgc acctgctttt gggatgttgg gaacactaat aggtttaata caaatgttag
    14941 ctgaccttac atctccagat ttaatagcat ctggcatggg aaaggcatta attacaacgt
    15001 tttatggttc tttacttgcc aatatcgctt tgaacccaat tgcatataat atagatgaga
    15061 agactgaaaa agaaatttat gtaaaggaaa tgatgttgga aggtataata agcatacaat
    15121 ctggggagag cagtatagta gttgaggaac gtttagccac atacctttca aacaatgaga
    15181 agttagaaat tatgaaatcc aataaaaata cagaaagagc aatgagcaat ggcgcgtaaa
    15241 aaaaataaga aaaaggatga gataaatcca gatgcatggc tggctactta tgcagatact
    15301 ataacactga ttcttacatt tttcgtatta ttgtatgcaa catcagttgt agatatggaa
    15361 aaatttaaaa acatagcaga tgcgttaaaa ggtcagtttt ctgggacaag tatatttagt
    15421 acgggttcaa gtacaagtga gaaatctcca atagacagtg ttttagtgga taaaaatgat
    15481 aaatcaaatt tagaacagga tttaaaagat aaagttaata attctaactt acaaaatagt
    15541 gtaacagtca aagatgatag tagagggatt ttactagagt tagatgattc aattttattt
    15601 gactctggga tttctgagtt aaaagtagat agtaaagcta ctcttaataa agtatatgac
    15661 atgataaaga tgatgaaaaa taagatagtt atagaaggcc atactgataa tgtacctgta
    15721 aaatcaagta cacatgaatc caattgggat ttatcttcta gtagagcagt aagtgttgta
    15781 agatactttg tagaagaaaa gggcatggac cctagactat tttcggcaac aggatgtgga
    15841 gagtatcaac ctttggtttc aaatgaaaca gcaaagggaa gggctgagaa tagaagagtt
    15901 aatatactta taactattga ctaggaggaa gaggtttgtt caaaaataaa aaaattatta
    15961 taattatttt gatactgatt attttctcag cgggagcagg tttttttata ttaggagaag
    16021 gtggaaataa acaagttgac aagattgcag gtttgtttat tccaacagga aaaggtaaag
    16081 atgaagtaaa gaccgaaaat cttgacttag aagagatggt attaaagcta gctgatgaag
    16141 gtgtaagata tttaaaagta tcaataactg tatcttatga ttcttcatat aaagatatag
    16201 aaaaaaatac gcctttaatt agggataata ttataaattg ttttatggca aaaagggcag
    16261 atgattttac tgctgataat ttaaagaaca taaaagaaga aattaaaaat acattaaata
    16321 tacaactagg tgaaaatgta attcaaaata tatatttcac aaatatagta gtgcaataaa
    16381 aggatggtaa gacatgatac tttatttact aaaaatatta atatgtatcc caataatttt
    16441 aatattgata gtcttatctt taaaaattag ccaaggaact ttattaaaaa aatataatga
    16501 taaatatgta aaaatactag atattgtaaa tataagtaaa aacaatagca taataatttt
    16561 aaaaataggt gaagaaggat gcattatggc atcttcttct tctagcatga gtaaaataaa
    16621 agatttgacc aaagaagaaa tattaaaaat agaaaacgac attaaagaaa gaaattttaa
    16681 aattcaagat tttaaaatgg aaagaattaa aaagaaatat gatttcagta gatatattgg
    16741 aaaatttaca aaaaaggaag ataagtaaat ggaactatta tcaaaattac ctttggctga
    16801 tgtaccatat acaagttcaa tgcaattgtt tttatttcta actgcattaa tgtttttacc
    16861 atttgtaatg ttcatgatga caagctttgt cagaatagtt ataagtttat catttttgaa
    16921 atcagcatta ggatcacagc aagcaattcc aagtcaaatt ttggtaggac ttgcaatagt
    16981 attgacaata tttataatga gaccagtatt aaatgagata aacgaaaagg cattacaacc
    17041 atatatgaaa gaagaagtta ctatggaaga agctatgaag gaagcagaag gacctattaa
    17101 agagttttta ttaacgcaaa ccagacagac agatttagat ttatttgtag aacaggctgg
    17161 tttaaaagaa aagaaattaa ctcgtgaaaa tataccacta tctgttgtag ttccagcatt
    17221 tgctataagc gaactaaaaa ctgcttttca aataggtttt ttaatatata taccattttt
    17281 gataatagac cttgttgtgg caagtgtttt gatgtctatg ggtatgttta tgttgcctcc
    17341 agttatgata tcattaccat ttaagttatt attatttgta atggtggatg gatggaactt
    17401 gatagtaaaa acattgatat tgggatttgg ataggagaaa aatagtatga gtgaatttat
    17461 gataatgagt gttcttaaaa atggcatgtt tacaggagct atgattgcag ctccagtact
    17521 cattgtttct cttgtagttg ggcttttaat aagtgtgttt caagctacaa ctcaaatcca
    17581 ggagcaaaca ttgacttttg taccaaaact aatagctatt cttttagtag tagctattgg
    17641 tggaagctgg atgttacata ctcttattag ctttacttct gagatattca atatgatatc
    17701 tcttataact aaataagtag gatacatatg atttttgtat ttattagaat agtagcattc
    17761 tttggaacac taagaatact atttccagct ggtacacctg ctttatttaa gtcactcttt
    17821 gcaatcatta tatcaatatt gatttcaagt actatgcaaa ttgaatatgc aacaaatata
    17881 gacaacattg tattgtttac cttatatggt gtaaatgaga ccataactgg gattatgctt
    17941 ggatatataa ccaacctatg tttttatagt atcagaatgg cgggttctat gatggaccaa
    18001 cagatgggat tatcaatgat taatatgttt gacccaaatt caatgactca gacaacttta
    18061 attgataatc ttatgaactg gacagcactt atgatatttt ttagcatgga tggtcatcat
    18121 gttttaatta gaggtataag gtatagtttt gagttaattc caataggaaa accatttgta
    18181 gataataaca tagattatat aataaacata tttgtgcagt gctttttaac tggattcaaa
    18241 atagcaatac ctattgtact ttgtctgctt atggcagatt ttattttagg gcttatatca
    18301 agaagtgtac ctcaattaaa tgtaatgata gttggtatgc cattaaaaat attggttggt
    18361 attgcattat ttatcatatc aataccttta attgtcaatc aaataagtca tttattaagt
    18421 caaataccaa agatgtatga gggaactttt gcactagctc ctatgttttt tatgggttca
    18481 gcagataaaa cagaagaagc tactccaaag aaaaagggag aacaaagaaa gaaaggtaat
    18541 atagcaaaga gtagagaatt acccgttgca atgactctac ttgcattcac cttattagta
    18601 cctacattat tttcatatgt agtagataca cttaaatctt cgcttaatta ttttttgagt
    18661 ctagattttt atatgaacat aaattattca aacctagaga aattagttat agcaggatta
    18721 atggattttt ttaagatatt tttaccaata gctattccct ttttagtttt aggcataata
    18781 gcaaacctat ttcaggtggg aatactgttt actggtgaaa ctttaaaacc aaatttatca
    18841 aaattaaatc caattagtgg attcaaaaac atgttttcta tgagaagttt gtcaacctta
    18901 ataaaggata cagcgattat atctattttg gcttacatag gatatacatt cttccaagat
    18961 aattatttag atatattaaa gctaggtaac atatacctac caactttaat gtacacagtt
    19021 aaagacttag tatattcaat attgagtaag atttgtgtag ctatgatagc tatagcagtt
    19081 gcagattatg tttatcaaag atacagtcat aaaaagcaac tgagaatgac aaaacaagaa
    19141 gttaaagatg aatataaaaa ctcagaagga gacccagaag taaaagctaa gataaaacaa
    19201 aaacaaagac aaatttcgtc tcaaaggaca atgcaagcag taccaagtgc tactgtaata
    19261 gtaaccaatc caacccactt atctatagca gtgagatatg aaaaagggaa agaccaagct
    19321 ccagtagtgg tggcaaaagg tgcagattat ttagctttta aaataaggga aattgcaaag
    19381 ggaaatgaca tccctataat agaaaacaaa cctattgcta ggttgttata taagcaagta
    19441 gaaatagacc aagaaattcc agaggacatg tatcaagctt ttgcagagat attagtggca
    19501 gtatataaaa tcaaaaatag atacaaagtc cccaaaaggt aggataagta atgaataagt
    19561 ttaatttaaa ggaaaatatg gatgtaatag tggcatttgg tgttgtaggg attgttctta
    19621 tgatgatagt gccattacct acatttatat tagatatttt actggcaatt aacataagtt
    19681 tatctgtgct aattttacta atgacattat ttacaacaaa tgtacttgat ttatctatat
    19741 ttccaacagt gttacttgtg acaacactat ttagattggg attaaatctt tcatcaacta
    19801 gattgatttt aacacaggga tatgcaggtg aagttataga ggcatttgga agttttgttg
    19861 taggtggaaa ttacgtagta ggaataatta tattcttaat tatattaatt gttcaatttg
    19921 ttgttataac caatggttct ggaagagtat ctgaagtaac agctagattt acgttagatg
    19981 ccatgccagg taagcagatg agtatagatg cagatttaaa tgcaggagtt atagatgaca
    20041 aaactgctag aaaaagaaga aaagagcttc aacaagaagc agatttttat ggttctatgg
    20101 atggagcaaa taagtttgta aagggagatg ccatagcagg tcttatagta acagcaataa
    20161 acataatagg tggaattgta ataggagctg taatgctgaa tatgactctt atgggtgcag
    20221 cacagacata tgtaagactt actatagggg atggtcttgt aagtcagata cctgctctat
    20281 taatatctac atcagcaggt ataatcgtaa caagagcttc tactgatgaa aactttggtg
    20341 gattagtagg taaacagtta acggcaatac caaaagcagt aatgatgact ggttgtgttc
    20401 taatcgtact tggtatgatg cctggattac ctaaaccagc atttttttca ctaggtatag
    20461 gagctatagc agcaggttac tttttaggaa gagaaaagaa agatacttta gaagacttgg
    20521 agtttgaaaa acctgatatg gtaaacccag ataaaaatga agtagaagat gtaacaaact
    20581 taataaatgt agaatcaata gaagtagaaa ttggatatgg tttaatatct ttagctgatg
    20641 aatcagcagg aggagattta cttcagagaa tagcatcaat aagaagacaa tgtgcaatag
    20701 acatgggtat agtcgtacaa cccataagaa taagagataa cttacagtta aatccaaacc
    20761 aatacaatat aaaaataaaa ggaaatgtag ttggtgggta tgagataatg cctaatatgg
    20821 ttttatgtat ggatcctatg aatcaaggat ttagcatatt tggaataaag acaattgaac
    20881 ctacttttgg aatagaggca ctttggatag aaagtgacaa ggtagaagat gcagagttaa
    20941 agggttatac agttgtagat tcatccacaa tacttataac tcacttacta gatataataa
    21001 aatcaaatgc acacgaatta ctgggaagac aagaagttaa gactattata gatgcagcaa
    21061 gagaaaatta tagtgctgta gtagatgaat tgataccaga tttgatgaca cttggtgaaa
    21121 ttcaaaaagt acttaaaaat ctgttaagag aaaaagtaaa tataaaggat agagttacaa
    21181 tacttgaaac gcttgcagac cagtctagaa atacaaaaga tatagagctt cttactgaat
    21241 atgtaagaat agccttggct agaagtattt gtaataattt agttgatgat gataagacaa
    21301 ttgtagtggc tacattgtct ccagaaactg aagagttgat atctaataat ttacagaggt
    21361 ctgtaaatgg aacttatcca gcagttgacc cagaaaatac aaataaaata tttgaaagta
    21421 tacaagaagt aatgagcaat gtatatttca atcataatat accagtaata gtagtatcac
    21481 caaagataag agctccattt agaaaattag ttgaaattgt atatcctaat ttgactgtat
    21541 tatcattaaa tgaaatacct aacgatatta aaatcaaagc agaaggtgtg gtgagcatat
    21601 atgatgacga aaaagtatac agctaacaca attcaggatg ctatgaatct agccaaaatg
    21661 gaattgggtg acaatattac attaatagat aaaaaagaag taagaaagtc tggaatacaa
    21721 ggtatctttt caaagaaaga tattgaacta actattggat gggaaaagag agaaaacgta
    21781 gaacaaaaag atttaaaacg agagatagaa caattaaagt caattattaa caatatgggg
    21841 tttgataata agaatgataa tgatatagat aaaatatgta aaaatctact aagtttagaa
    21901 ttgaatgaag aaattgtaga atctataaga attgatttgc aagaaatgaa gttcaatgga
    21961 atcgatacaa gtaaaaattt agtagaaatt ttaaagaaaa agattaaaat agaaaatcaa
    22021 gtaataaatg gtaaaattgc tctagtcgga ccaccaggag taggtaaaac gactacaata
    22081 gcaaagttag ccgcaaaatt agtttttgaa gaaaataaaa aagtaggagt aataactata
    22141 gatacataca ggattggtgc tgtagaacag ttgaagatat atactgatat aatgaatatt
    22201 ccctttaaag gggtaataag tcctgatgaa atggaattgg cacttgacga gatgaaagat
    22261 tgtgatgttg tattaataga tacaacaggt agaggctaca aaaactctat gcaaatacta
    22321 gaaatcaaaa atcttataga taaagctgaa actgataata tacatttagt tttgaattgt
    22381 acaacaagag aaagtgatac aaaagcaatt atagattcat atagaaatgt gaattttaaa
    22441 agcttaataa ttacaaagct agatgaaaca ataacatatg gctcaatatt taacataatg
    22501 aattatgcac aaaaaccaat atcatatatt acaacaggac aaaatgttcc tgatgatatt
    22561 ataaaaccta atgaagataa gattataagg ttactattag gagttgaaag tatatgatag
    22621 accaagctca aattttgaga aaaatagcaa tggaaaaaag aggacttgat gagtttatag
    22681 tagagaatga gaatgtacct aagataatta caatagcttc tggtaaaggc ggtgttggaa
    22741 agagtaatct cgctacaaat ttgtctatat gcttgaccaa acttaataaa aaagttctta
    22801 tattagatgc agacatagga atgtctaata tagatataat aatgggggta aatgtaaaag
    22861 ggactataat tgatgtaatt aatggcgaaa aaaatataga agatataatt tcacagacta
    22921 agtatggtgt aaatataata tctggaggtt ctgcccttaa tcatatagaa gactttacag
    22981 aagctcaaag aaataagttt atacatagca tagaacaaat tcacgatgtg gattttataa
    23041 taattgatac aggagctggt atgagcaaaa gcctattgtc atttatatac tgtagtactg
    23101 aatttttctt aataacaact ccagaaccga catcattgac tgacgcatat agtttattaa
    23161 aagctataag taattttggc ataaagagaa gtgcaaatat aataatcaat agggtaattg
    23221 atttagaaga agcaaaatct acatacaaaa gaataaaaat ggttgtggac aaatttctaa
    23281 accttaatct agaattgtat ggatatataa tagatgacaa aaaagtaagt ttatgtgtaa
    23341 aaaaacagat accattcatt ttagaatatc ctacaagtat tgcatcaaaa tgtattatag
    23401 ccatagcaaa gagaatggta agtcagtcaa gagaagatat tgttgataat aaaagttttc
    23461 taagaaagat gtttagtata tttaaaagct aaggaggcgt agttaatgaa tagagaagaa
    23521 ttaataaaag agaatatgcc tcttgtaaag agtatagcaa gaaaattttt tataccaggg
    23581 aaaggttttg aatatcaaga cttagtaaat agtggagtca ttggattgat tgatgcaata
    23641 aataaatttg atgctgaaaa aggggcaaag ttttctagtt actcttatat aaaaataaaa
    23701 tctgctatat tagatgaaat tagaaatcaa agtccaattt ctaaacataa tctaactaaa
    23761 gtaaataagt ataatagagt ggtagaaaag ttgcaatcta aattattaag agagccaact
    23821 tcatatgaaa ttgcaaaaga attaaaggtt tcagaaaaag aacttcatga tatcgaaagt
    23881 aatatagata tgttaaatat tgtatcatta aactatgtaa tctttgaaga tacaaatgaa
    23941 actgtacaag atgtaataag tgatagagaa gaggaagctc cagaaaatat aatagaagaa
    24001 gaagaaaagt tagagattct atccaaagca attagtaatt taaatgaaag agaaaaatta
    24061 atactttcac tgtactatta tgaagattta aatctaaagg agatagggaa agtgctaggt
    24121 gtttcagaat caagagtttc acaactccac agaaaatcaa taagaaactt aagaaacaag
    24181 ataaaagaac ttaaatattc tatatagatg gtgattgata agatgataca aattatatta
    24241 atagtagtgt ctattttaat aatagtaact atagttataa atttacataa ggaatcaaaa
    24301 aataaagata acagatactt tgataaaata tttgagcaag aatatgaagc aaatgataga
    24361 ttaataattg cagaaaaaag aagattcttt ttagacaaag tacttgcaga aaaaagaatt
    24421 aatagtcata taaaagttta tgaaaattat aacctaataa aggctaatga agaaagaaag
    24481 gaaaacatat ttatagaatc aaaaaaggaa gtaagacaag aaaatgagtt atctcttaaa
    24541 gtgaaacatc ttataagttc aggttatgat gatgtggaaa tatgtaagat gctagatatt
    24601 ggaaaggggg aactgttatt aataaggagt tgttacaaaa tataaggata ttattatgtg
    24661 ataaaaaagt attcttagga ataggtattg gtatattaat atcaacttta tgtataatgc
    24721 tttttagtaa taatgatatg agtaaggcag agatagaaaa aaaggccaga gcgttaggca
    24781 tgaaatatac aagtgagatg aaagttttag aatagatgat attgtttaga catttacaaa
    24841 aaaggaggtc ataatgtgat aagaggatta tatacagcag tttctgctat gataactaat
    24901 caacaaaagc aaaatgttgt tgtcaataac ctagctaata tggatacaaa tggatataaa
    24961 agtaaacaac ttttgactaa aagttttgat gaattaaagt tggagggtta caataactac
    25021 gcaaatggaa ctaaacaaaa gcagattata ggagatataa gtccaggtat ttctatggat
    25081 gaaactatca caaattttaa tcaaggacct ataaaaacta ctgataataa aatggatgta
    25141 gctattcaag gcaagggatt ttttctagtt agtgatgcag ctggaaatca attttacact
    25201 agaaatggga acttcagaac agacaatcaa ggtgacctta taacaagtga agggtattat
    25261 gtacttggga cgaatactgc tactggtgct gtaggtcata taaatgtagg aaatcaaaaa
    25321 tttgaagtgg caaaagataa tacaatatca ctagacggaa atgctatgta taaatttaat
    25381 attgtagatt ttaataacta caataactta aaaaaagaag gagataactt atattctgga
    25441 ggaggagcta tgaaagtaaa taatgcactt acaatacaaa gtgcgataga gtcctctaat
    25501 gtagatatgg tatctgaaat taataatatg atgacaatat caagagaata tgaggcaaat
    25561 caaaagataa ttcaggctat ggattctaaa ttagcaaaaa tagcaagtga gattggttca
    25621 gtaagatagg taataggagg tgaattttta tgtacacaat gttttatact agtaaaacag
    25681 ctttatcagc aaatcaaagt aaactagata taatatctaa caatatagca aattctgatt
    25741 caacaggata taaaaaaata aatatggaat attctgattt agtggatgaa gtactaaata
    25801 gaccatcata tcctacaaat ggcaatgata tatcgactgg aacaggagct aaggcaacag
    25861 ctcccatgag aaattatgaa caaggtgcat tggctcctac agatagtaag agtaatttgg
    25921 caatagatgg agaaggattc tttagagtga taagaaaaga tggtacatat gcatatacaa
    25981 gaaatggtgg atttaatgta gatgctttag gtaagattgt agatgataat ggaaatatat
    26041 tagatgtcca atttgatgca ggatataact ataataatac aaatatagat agtacaaacc
    26101 tgacagtttc aagaaatgga gagctattcg tagataataa aaaaatagga aaaatcaact
    26161 tatatcaacc tataggaact caaaatttta tatctgaagg agatagttta ttcgtcgcaa
    26221 ctgatgcagc acagattaaa caagttgaga aagtcgatat agttcaaggc tatagagaga
    26281 ggtcaaatgt ttctttacaa gaagaatttg ttgacttaat agccactcaa agagcatttc
    26341 aaatgaactc aaagggaata aagacagcag atgaaatgtg gcaaatagcg aataatttaa
    26401 gagctaaata attatgtaag gtgaggaata ttcatgaatg atatgaaaga gtacgatttt
    26461 ggcaaacctc agagttattc aagagaaaat ttaacttgtt taaatttttt attagctgaa
    26521 ttttgtaaaa aatataaaaa ctatataatt tatgaactta agtgtaattc tagtatggtt
    26581 gttgatagta tagaccaagt aaattatcag acttttttag atagagtaac ttcttcatgc
    26641 gtagtagtac aaaatgcaat ggaacctgtt atgaaaaact tttcttttag aatagataga
    26701 agtgtagctg atatgtggat tgatataact tctggaggaa ctggagttgt aaaggataat
    26761 gatagagagc ttacagaact tgataaaaaa gtgcttttac accttatgga tgatttagta
    26821 agaaatatgc acttatttga gggttttgaa aatatacaag tattggatac acatacacat
    26881 attaatttgc cacaattatg ttctccaact gcaccagtat gcgttgtaga tttaaaagtg
    26941 ctaaatgata atgagtatat tggaagtgtt tctctttgtt ttccatacaa tggtttagag
    27001 gctctgttag aaagtatatc tgttatagaa ttttttgata atgatgtagg agatgattca
    27061 gaggaattta cccaaaaaat ctataatagt gtatctaata tagaattatc agttattgca
    27121 gagataggaa aagtatcaat taatgttgag gatttactta aactagaagt tggagatgta
    27181 atagtaacaa ataaaaaaat aaatgattat atagatatat ttgtagaaaa ttcaaaatca
    27241 tacacagcaa caccaggatt tatatcaagt aaaaaaggtg taaaaataaa tgatgctgtt
    27301 ggaaaagagg tgtagttatg gacaatagta atcttatttc agatgaagaa gcaaagatgt
    27361 tattaaatga taaagttgaa agtgaagttg atggagatga tgagttagcc ttagatgtct
    27421 ctgaagttaa tcgaaataat atacaaagga tacttgacct taagttagaa ttatctgtag
    27481 ttataggaag aactaagatg actctaagag atattttaaa tttacataaa ggttctctta
    27541 ttgaactgga tactttagca gaccaagatg tggaaatact aatagataat aaggttttgg
    27601 cttatggaaa agtagtggtg gtagatttaa attttggtgt aaagataaca agtatagtaa
    27661 gtgaagaaga catggtaaaa tctcttgttt agagataaca tcaattaata cttaaggcat
    27721 gaggtggtac ttattttaaa cttaaatgat ataaaaaaag atatactaaa actcaataaa
    27781 aaagactcat gttctctata taatgaatct attaataata taaataataa ttgtatatca
    27841 actggattaa aatttttaaa acaagcaaga gatttaaacc cagatgatgt tgatatatta
    27901 aatctcacag gtcttgtaaa cttactaaaa tgtaattttg atgatgctgt agaaagtttc
    27961 tataagagtt cactttgtga aaaaactgct ctgtgtgaaa agtatataaa catgcttaca
    28021 tctgaggagt ttttaatttt ctttgaaaag tacaatcaac ttataaggtt aataaataaa
    28081 ggaatgtata aagaagctat acaagggttt aaactcataa ctgaccaata ctgtgattta
    28141 atagaacctt atgagttact tgccttactg tatgacaagg aagaagaata tataaaattt
    28201 gatgaatgtt tagaagtatt gaagattatt gataaggaaa attatttatt aaatcaagaa
    28261 aataacacat tagatatgta taatagtgct aatatacaaa ctaaaagtaa tataaaagta
    28321 gatgattata agaatgagtt tttacataaa agtagtagta atcataaaat taatgattat
    28381 aataatgtaa ctaaagaaaa gagttcaagc aaaaaccaga aaagttcaga ttctaataag
    28441 a
//
LOCUS       DAEMIK010000035        28426 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEMIK010000035 DAEMIK010000000
VERSION     DAEMIK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.035"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            109..738
                     /locus_tag="KPK87_001321"
     CDS             109..738
                     /locus_tag="KPK87_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2537487.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(852..2366)
                     /gene="glpK"
                     /locus_tag="KPK87_001322"
     CDS             complement(852..2366)
                     /gene="glpK"
                     /locus_tag="KPK87_001322"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG2537488.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2610..3152
                     /locus_tag="KPK87_001323"
     CDS             2610..3152
                     /locus_tag="KPK87_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2537489.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3166..5658
                     /locus_tag="KPK87_001324"
     CDS             3166..5658
                     /locus_tag="KPK87_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2537490.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6079..6477
                     /locus_tag="KPK87_001325"
     CDS             6079..6477
                     /locus_tag="KPK87_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2537491.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6694..6903)
                     /locus_tag="KPK87_001326"
     CDS             complement(6694..6903)
                     /locus_tag="KPK87_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537492.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(6935..7144)
                     /locus_tag="KPK87_001327"
     CDS             complement(6935..7144)
                     /locus_tag="KPK87_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537493.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7443..7862
                     /locus_tag="KPK87_001328"
     CDS             7443..7862
                     /locus_tag="KPK87_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537494.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8090..8545
                     /locus_tag="KPK87_001329"
     CDS             8090..8545
                     /locus_tag="KPK87_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537495.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHIKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(8753..9013)
                     /locus_tag="KPK87_001330"
     CDS             complement(8753..9013)
                     /locus_tag="KPK87_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537496.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(9276..9629)
                     /locus_tag="KPK87_001331"
     CDS             complement(9276..9629)
                     /locus_tag="KPK87_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537497.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(9646..14967)
                     /locus_tag="KPK87_001332"
     CDS             complement(9646..14967)
                     /locus_tag="KPK87_001332"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537498.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKFINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTFT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIITTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(14984..15967)
                     /locus_tag="KPK87_001333"
     CDS             complement(14984..15967)
                     /locus_tag="KPK87_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2537499.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(15968..16666)
                     /locus_tag="KPK87_001334"
     CDS             complement(15968..16666)
                     /locus_tag="KPK87_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG2537500.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(16663..17715)
                     /locus_tag="KPK87_001335"
     CDS             complement(16663..17715)
                     /locus_tag="KPK87_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2537501.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(17708..18136)
                     /locus_tag="KPK87_001336"
     CDS             complement(17708..18136)
                     /locus_tag="KPK87_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2537502.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(18136..18462)
                     /locus_tag="KPK87_001337"
     CDS             complement(18136..18462)
                     /locus_tag="KPK87_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG2537503.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(18478..20007)
                     /locus_tag="KPK87_001338"
                     /pseudo
     CDS             complement(18478..20007)
                     /locus_tag="KPK87_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            complement(20289..20711)
                     /locus_tag="KPK87_001339"
     CDS             complement(20289..20711)
                     /locus_tag="KPK87_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537504.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(20711..23164)
                     /locus_tag="KPK87_001340"
     CDS             complement(20711..23164)
                     /locus_tag="KPK87_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537505.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(23164..23331)
                     /locus_tag="KPK87_001341"
     CDS             complement(23164..23331)
                     /locus_tag="KPK87_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537506.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(23358..23804)
                     /locus_tag="KPK87_001342"
     CDS             complement(23358..23804)
                     /locus_tag="KPK87_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2537507.1"
                     /translation="MSENGLLKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(23930..24358)
                     /locus_tag="KPK87_001343"
     CDS             complement(23930..24358)
                     /locus_tag="KPK87_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2537508.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(24372..25436)
                     /locus_tag="KPK87_001344"
     CDS             complement(24372..25436)
                     /locus_tag="KPK87_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG2537509.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(25441..25884)
                     /locus_tag="KPK87_001345"
     CDS             complement(25441..25884)
                     /locus_tag="KPK87_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537510.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(26133..26573)
                     /locus_tag="KPK87_001346"
     CDS             complement(26133..26573)
                     /locus_tag="KPK87_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537511.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(26678..26875)
                     /locus_tag="KPK87_001347"
     CDS             complement(26678..26875)
                     /locus_tag="KPK87_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537512.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQS"
     gene            27044..27364
                     /locus_tag="KPK87_001348"
     CDS             27044..27364
                     /locus_tag="KPK87_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2537513.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVVAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            27446..27952
                     /locus_tag="KPK87_001349"
     CDS             27446..27952
                     /locus_tag="KPK87_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537514.1"
                     /translation="MNNLDKLFELASQEGIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT         9368 a         4739 c         3185 g        11134 t
ORIGIN      
        1 atgcacaata tttgtacata ttgtagacat ataaacctat aaattataca atagtattgt
       61 taagattata tattttttta acataaattt tgtattgggg gaaaaacaat ggataaaaac
      121 tatacgatgc caatatacca aaaaatagcg ttggatattg caaataaaat atacacgggt
      181 gaaatacaag aggattcagt actatttgga cgctctgttt tggctggtaa atacaatgta
      241 tctccagaaa cgataagaag agctgtcaaa attttagagg acattggcgt tgttaaaagc
      301 ataaagggta aaggtgttat tgtactatct ccagacaaag cttcttcttt tataaaaaaa
      361 tatagagata ttacaaatat atcatcatat aaatcaacac tttataattt aatagatact
      421 aaatctaatt tagaaaatga aatattagat actataaata aaattttaga ctattctaac
      481 agacttgaaa taatcaatcc tttagtgccc gttcagttca ccataaattc taattgcaaa
      541 tatattggtc aaacagctgc acaaactaaa ttttggcaaa atacaggtgc tacaatagtt
      601 gcaattaaaa gaggtgaaga acttataata tctcctggac catatattga atttttagaa
      661 ggagacattc tcttagtagt tggtgaccaa cacatttaca attctatacc aatgttttta
      721 tatgaaaacg aaaaataaat tatataattt agtatgatta tataaaaatg caaaattaac
      781 aacaaaaaaa gagcttcata ttttacataa agctcttttt aaattcaaat aaatttaact
      841 ttgattaatt tttaaatatc caattcatct tctttttccc agcttaaagc tctcgataca
      901 gcttttttcc atcctttata taatttacat ctcttctctt cactcatatt tggattaaac
      961 tctttttcta ctgaccactt tgaagtaatt tcatttcttc caatataaaa tccaacagac
     1021 aaccctgcta aataagcagc tcctaaagct gtagtttcta ctattttggg tctatctata
     1081 tctacattta atatatctga ttgaaattgc attaaaaaat tattattact tgctccacca
     1141 tcaactttta atgattttag ttttaatttt gaatcgtttt gcattgcttc taaaacatcc
     1201 tttgtctgat aagcaattga ttccaatgtg gctcttacta tatgttcttt ttttgctcct
     1261 cttgtaagtc ctaaaattga acccctagca tacatatccc aatatggagc tccaagacct
     1321 gtaaatgctg gcactacata tacaccatta gtatcctcta ccctatttgc atacatttca
     1381 ctatcttttg cagattcaat tatctttaat tcatctctaa gccattgaac agatgctcct
     1441 ccaataaata tacttccttc taatgcatat gttacttttc catcaactcc ccaagcaatt
     1501 gttgtaagta acccatgctt agatttaact atgttactgc ctgtattcat aagcaagaaa
     1561 caacccgttc cataagtatt ttttgcagta ccttcttcaa cacaattttg accaaataat
     1621 gctgcttgtt ggtctccagc acatcctgca ataggtattt tagctcctga taacatgcgc
     1681 tcatctgtat gaccataaac atagctagaa ggttttacta atggcaacat gcttattggt
     1741 atgtccaatt ctcgtaatat atcctcatcc cattctaaat catttatatt gtacatcatt
     1801 gttctagatg catttgagta atctgttaca tgtacttttc cacgagttaa attccatatt
     1861 aaccatgtat ctatagttcc aaataataac tctccttgtt ctgctttttc cctagctcct
     1921 tctacattat ctaatatcca ttttactttt gttgctgaaa agtatgcatc tattaaaaga
     1981 cctgtttttt cttttattga ttcttccaat cctttatttt taagttcatc acatatttct
     2041 gacgttctcc tacactgcca tactattgcg ttataaatag gtttaccagt atacctactc
     2101 caaacaaccg tagtctctct ttgattagtt atccctattg aagcaatttc ttctggtctt
     2161 atacctgctg tttctataac ttctctcatt accccacttt gtgaccccca tatttccata
     2221 gggtcatgct ctacccatcc agcttttgga taaatttgat tgaactcttt ttgtgcaatt
     2281 ttcactattt cgcctttttt attaaatagt atagctcttg aactggttgt tccttggtct
     2341 aatgccatta catacttctt ttccatcacc atattctcct tatcatataa tatttaattt
     2401 attaaaaaga aaaatttaat atcacatatt tcaataaata atcatactta aatacaaatt
     2461 taatataata gatatttagt aagaagacac ttattttcta tcatatctat ataataatat
     2521 tatacattat agcttacata ttgattaata aattttaaag gagtataata aacagtgttc
     2581 ataataattt taataaaaga ggtggtaata tgccaaagat tttagaaaat gtaaaagaag
     2641 atatattaaa agtgtctcgg gatatgattt tagaagaaga ttactcgaat attagcataa
     2701 ggaaaatagc tcaaaggtgt ggtataagtg cagggacagt gtataattac tttaattcaa
     2761 aacaagaaat aatagaatat atcacaaaat ctgaatggga tttattgata aggcgaattg
     2821 aatactccaa taaaaacact gaagattaca taaaaaaact aaacattatt tttactgaaa
     2881 taagaaattt tataaacaaa gttcataata tccaatacaa tgacttttta aacacttttg
     2941 aaacagagag attttttgaa atgaaaaaac acaaagacga ctttcatgaa cagctttcaa
     3001 ataaagtata tgaagcttta gaaaaagaaa gtttttttaa taatgacaaa ttagtatgca
     3061 atatcataat aaaaatgttt ttttcatatt caacatatct tcatatagaa tttgaagatt
     3121 taaaacccta tattgaaaaa ttaatacatt aaggtggtgt ttaccttgaa tcataatact
     3181 agtattaaga acaaggacag taatccaaaa atatttttag tacttacact ttatctatta
     3241 ggtatattta tgggagctat tgatactgga atagtatcac cagcaagaac gattatacaa
     3301 aatagccttg gagtaaatga aaaaacggga atttggatga ttaccatata cacattaaca
     3361 tacgcttctg taattcctat ctctggaaaa ttagcagata aaataggaag aaaatatgtg
     3421 tatctagtta gtatatttct atttggtagt ggttcattaa tatgtggatt atcatcacta
     3481 ttttccaatt tctatatttt attaattggt cgtgttattc aagcaattgg tggtggtgga
     3541 atcatgccaa ttgctacagc agaatttgga actaccttcc cagaaaacaa aagaggcatg
     3601 gctttaggtt tagttggggc aacatatggt attgccaata ttttaggttc aagcattggt
     3661 agcacaattc taagtatctt tggaactcaa aattggaagt ggctattttt tgtaaatctt
     3721 ccaatttgtt taatcatact tattggtgga gttttctgta ttaaaaacaa taagtctgaa
     3781 tctaaagaaa aaatagataa gcttggtaca ttgatgttga tatgtataat cgtatcacta
     3841 ttatatggtc ttatgaatat agactttttt aattttaaaa attctataca agatatatca
     3901 gtttatccat acttattgac atttattgta cttataccta ttttcatatt tattgaaaat
     3961 agagctaaag accctatttt aagctttgaa tattttttaa atccgagaat attaatagta
     4021 cttattttat ctttaattgt tggtattgga atgatgggta tggtattcgt tccacagtat
     4081 gctgaaaatg cattaaagat aaatgctggt tctggtggat attttgtagc tatattggga
     4141 ttatttgcag gtattgctgc tcctttaagc ggaagactta ttgacaagtt tggggcaaaa
     4201 aaaatactat tactaggatt ttctgtatct atgataggct ctctttacct agttttaatt
     4261 gctctaaaaa ccaatactgt atttagtgta tgtgtaagtt taatatttat gggattagga
     4321 atgggattta ctatgggaac tcctctaaac tatatgatgt tatcaaatac aagattagaa
     4381 gaatctaatt cagcacttgc aacattatcc ttgatacgct caattggtac ttcaatttct
     4441 ccagctataa tgattggatt tatagcacat gcaggattat ctgtgcaaga taatattatg
     4501 gacttagttg gaaagccctc aactcctaaa atagttcaac ttgaagaact tgaaaacatg
     4561 atagatgact taaaatcaga ccctgaaatg gcaaaaaatt taaaaaatgt ctctatccca
     4621 aatatgaatg atacttcaaa tataaatatg gacatgacag gtggtaaact tccaaaatcc
     4681 ttacttaata aagttcagtc agcagataca accaacatta ccaatataac aaaagaaata
     4741 tcaacaacaa tgtttgatga aaaagttcca tctataatta ctaaaataca gaaaaatgta
     4801 caaaaaggaa tagatggaac acaaagtgga ataaacggta ttgaagatgg tgaaaataaa
     4861 ctaaatagtg gtattaaagg agttcaaact ggtattgaca atatggtaaa agctagagct
     4921 ggtattaatc aaggaataga tggtgtaaaa aaaggtattg ctggtgttga taaaggtata
     4981 aaaggtatgg aacaaggtct tgaaaaacaa gatgaggcca tcaaggaact tacaactgct
     5041 tataataaaa tacctactac agaaaaaact cctactgata acgaaaataa ctcaaatgaa
     5101 caaaaccaga gtaataccag taattctgaa tcagatgaca ctaagaatga ttctacagaa
     5161 aataagaata acaattccag cgagaataaa ccaaatgtaa atgaacaaaa agaaagttta
     5221 aatgctcaaa tacagcaact aaaaaagaca agagatgact taaatacaaa gttacaaaaa
     5281 aataaaactc aaaagaacga attaagtaaa aaactaagat caatggaatc tcaaaagaaa
     5341 gagctacaaa gcaagttaaa taattctata agccaaaaga aagacatgga aaaaagcctt
     5401 gatacaatgc aacagcaaaa aaaatcactc caagcagttt tagcaaagac acaagaggta
     5461 aaaactgaaa taccaaaggc ttttgataaa tcaaaattgg attatataaa ttccatagaa
     5521 aataatagaa caaaaataga aaacacacta caatctactc taaactcagg atttaaacag
     5581 atgtatataa cagtattctg tgtaaatcta ctagcattta taatattatt gttctataaa
     5641 gaaaataaaa ctagataaaa gagtgttata gtattatatt gtaaatttat cttattctta
     5701 aatattttat tctaaagaat aaattcataa aatttttgta attgattaat ataaattaca
     5761 atataatact atttttctct taatcttttt ctcttttaca gacaatcaaa aagcaatctt
     5821 agtttatcga aataaattta tacaatctat tcatacaccc ctttacattt ttataacaat
     5881 taaacctatt gttatatata caaatcaaaa taaaccatat attttcaact ataatatgtt
     5941 aaaatcatat tatgagcata ttaatttaag caaaatgtca tttagacttt aatttagtag
     6001 ttttatatta taaatttata tatttttaat tttaaatgtc atttagacat tactaataaa
     6061 aagaaaggag tgtattttgt ggatacttta ggaaaaagaa ttgcttattt gagaaactcc
     6121 aaaaaactta ctcagcgcaa acttatggat attttaaaat ttgaaaactt aggcaagtat
     6181 gaaacaggag atagaaaacc taattgtgat atactaatgt caatagctga ttattttaat
     6241 gttacgactg actggcttct ctatggtaaa gagaaggtaa atgtcaatag tagtgtaaaa
     6301 gaagataaag aagattactt acatgttact aatgatgaaa tgacgatatt aaatctatac
     6361 agacaactaa acgaacgtga taaaataaaa atagaaggaa tacttgaact taagatatct
     6421 gaatataaag acttaaaaaa gcattcctct aataataatg aagataaaat ggtttaaaca
     6481 tacataattt gatacgattt tacatcatct attaaccttc tttttttgta gtatattact
     6541 aaaatatgga tattgaatta tttaaaaaca agtatagtct aatttcaagc acatttataa
     6601 tatttagatt atcttatcca tattttagtt taagtacttt gaaaaatata atacatctct
     6661 atgtataact tatattccta agaaatgcat tactcatgtt tttaattctt ccaacatctt
     6721 tctatacaca attgtacatc cttcataatt cccctcttta agaaggattg aacatgtact
     6781 agaatattta gttaaagtat ttaatctttc catatttaag ctttttatat tgctgagtgc
     6841 tgtaataaaa tcatcaattg ctctacaaca ctctataacc ccatctgaat ccaatttaca
     6901 cacaagaata ttcacctact ttatatacta tttattaatc atcttctgaa tattctatat
     6961 taagtaattg ataaattgat gatttatatt ttttacctgt tcttcttcca tttaaaatag
     7021 ctgacaaata gttttcatta acacctattt tcttcgctaa ttccctttga gtcatattta
     7081 attcaataag tctttgcttg acttgcactc ccaaaaaagg tatccatttt tttcgagcat
     7141 ccatttatca acttcctttc taagtgattt atatttactt ttatattatt ttagcttacg
     7201 caccccctct atttagtata tatttttaat atgtacacat attaagtata taaatttatt
     7261 taatactaaa taagattttt tgattacttt gtgctaaaat taggatgtaa attcgtatca
     7321 ttaagttaat tataaagtca ttttgacctt aagtcaatgg tttttatgtc attttgacct
     7381 ttaatttgaa actatttaca tcttaaaaag tctttttgac attaattgaa tggagagtgt
     7441 ttatggatac tttaggagaa agaattgttt atttgagaaa agcaaaaaac cttaaacaat
     7501 atgaactaga agaaatgttg ggctgtgaca acttaagtaa atttgagaga aacattagaa
     7561 aaccaaatta tgaaatatta aaatctatag ctgagatatt taatgtttca gtagactggc
     7621 tattaaatgg agataatcta tcacataaat cagatttaat ttgcgattcc tcctcaaact
     7681 atccacttaa tagcataaac tctaatgaaa taaaactttt aaacaacttt agaaagttaa
     7741 atgattatga taaagccaaa atagaaggta tgattgaatt aaaacttcat gaatatgaaa
     7801 aagagaagga tttaggaaaa attgagtata acaaaaataa agatgagaaa atagataaat
     7861 aatttttatg ttgcttttga tacgatttta catcatttct ttgttctaat atttttattt
     7921 catttatttt cttttttata attttatgat aaaataatta gtatattttg ctttacttga
     7981 atgtaattat ttatacaaaa gtcaaacata atataaatgt agtaaatttt tagtttcaag
     8041 taatatctcg ttattataac taaatattta ttaaaattag gaggataaaa tgggcttaag
     8101 agacaaattc gcgcaatcat ttgctagatc aaaaactatg agtggccctg agaaaaaggc
     8161 taatgaaata atgggtaaat tgcttcttaa gaaagctata ttacctatcg ttttaatgtt
     8221 cgtgataata atcgctggag ctatgcttaa aatcaattct tgggttacac ttggcataaa
     8281 tttagttatt gctgttggtg ctttcttcta cataagaaat tctagtaaaa agtatcagaa
     8341 ctttaaacct tatgttggca atttgattag tttagagaaa aaaggtaaaa aagagtacgt
     8401 tgctattata aaacaaggta agttaccagt taagttacaa atagcttatg gtggtgaaga
     8461 ccttgaacat attaagaaaa atcaaatggt tcaaataagt tataatccag atgctaaaat
     8521 agctattttg gttaatagac aataatttaa taaaaaaaag gagttgtctt aaaattgatt
     8581 ttttctaatc aaatttaaac tcaactcctt ttttaatttt tatatattat ttttattact
     8641 ttttttaatt aatattttta aatattaaat ccttctcaat aaaatagtga actatctaca
     8701 atactacttt atcttttcct aacaatatat aatctggtgc tactctttca tgttatacac
     8761 cgttttgtaa catacctttt atatcagtaa catcttcctt aatttcctct acatctgttt
     8821 tcattattcc catttgaaca agtatatttt tgtttatttc ttgttgttgt gtagataatt
     8881 ctataaaatt ttctacagtt tttttataca tgtctctatc ttcttttttc tcctgcatag
     8941 tatttttaaa taatagagca catagtatac ctattgcccc tagactactc aattcggtta
     9001 ttaatttatc cattatagcc tcctattgtc taaatttttt atacttatag ctttaatttt
     9061 tgttgtattc ttacatcatg caatattata ttttcaggat tatcaattta atcttctgta
     9121 ctttaaattg taggtgtaat ttatagattg aataaataat aaatatatat acaatttcta
     9181 atataacaaa aaataagtac aaggcttact tattaaagtt aagaccttgt actttacata
     9241 aaaaaaataa ctagtatata gttattctaa atttactatt caactggtat ttctaattct
     9301 ttacatctaa ttctaacttg ctctctaatt actgctggta cttcttctat tgtttttttg
     9361 cctcttaaaa ttaaagctac atatacttct accattccac tctcacctcc agattttctt
     9421 acactaaaag tgcttatatt acttgcttca ttgacagaac tttgatttaa caacatctca
     9481 tatatttctg catttgctat aaaattattt gttatatcat catcttgctt tacttgacct
     9541 atacttatgt ctgagatatc tttatactgc atttctactt ttctaccttg tttgatttgt
     9601 tctattttat ttttatcgaa tttaaacaat tttcacacct ccattttaag cttgtggctc
     9661 gttgttttca gcatcatatc taaaaatcat agatggacgt tttatagctg tccatttatc
     9721 tcctttttct actatgattt taggctgagc actttttatg ttggcttctt ctaattcata
     9781 gatttcttgg taacttgagt ctgatttttt gatttcaact agtttcatgt ctttgaataa
     9841 gtcagattta tttattttta cttgaacttt agttttattt attaattgat atataggatt
     9901 gttttccatc atgtcatcct ttaatccctc atttaatgtt actattaaat cagtgttatt
     9961 ttcagtaatg tttatgattg aatatacttt attgtttatc attaaaatag aatccttttt
    10021 tactttaagc cctgtttctg atttaaatat aaacatactt gaatataaat cgcctgtagg
    10081 actactggct ttaatcagta tcttattttc tcctaatact attttatttt gtggtatctc
    10141 aaatataatg ctatttattt ccaaattagt cttagtagat attaattcat catttataaa
    10201 catttcaagt ttttctataa cattattagg atcttgtact acaactctca cttgagatat
    10261 tgatttttca actatttcta tagatgcttt acttgtatct acttttgtta tcatgtatat
    10321 tgtatcatct gaaagctcta tatctaaaat atcatttaga tgttcaggaa ttggtatttt
    10381 agtaaattca tataaatcag taattgatat agatatttta tacatagatt tatttttacc
    10441 agttatccat agactattat catttttaat tactagtatg tggtcatcat tatgtgactt
    10501 aatttcttta acattagtta atactttagt aaaagttgtt attattttat ctttttcact
    10561 ttctcctagt ccaaacaatc catttgtatt atacccttga gcgtacaact ctccctcatt
    10621 agttaaaaca agtgtcattg agttatatat acttatatct ttaacagaag gtaatccagt
    10681 aaccttttgt aactctctta catcattatt gtgaccaact cccaattggt actcagtatt
    10741 ctttccatat gaccatagag tattatctgt cttaataata taggagtagt tatctccaca
    10801 agcaactttt ttgacatctg atatatttac ttcagtaaaa gttaataatt ctatatcttc
    10861 aagagtttga ttaatttctt tatatgaatt acttcctatt ccatataact tgccatcctt
    10921 agcaacagcc aatgaatgtg tttttgatat atcaatatca gatacctcaa atggcaatat
    10981 tattgaagta tacttaaaat tatctttaaa tatgttttta taatttatat acttattatt
    11041 tgtttttata tacttatcat taaacattac tagcttttca tttttcttat taaaaactat
    11101 tttatctgat gtgttatctg ttttaaaata tgaatgtagg gaaccttcaa gagcttcatt
    11161 tttatcatta tcatatctat ggtgaatacc taaatgatta tatctattat ctcctgttga
    11221 agaataaaat tttccatata tatctttaat aaatatagta ttgtaactag aaaatacagt
    11281 atcaacatct ttctttatag atactttata atatggaact gacatatccc tataagattc
    11341 atcagatata ccataagtat ctctacctga tgatatcaaa ttgttattat tatctatata
    11401 aagtgtatta ttagctgtta caaaaacatc tttaacatct tccacactat ttgtattatt
    11461 attatttgct atacttccat atccactaag acctccaaca aatagtttat tgtcatttgt
    11521 taaaatggct gaagtatatc cattaactac tatttttttt gcatttataa tattattaat
    11581 tactcgtatc ccctttatat cattagtctc tgttgaaaag aataccctct cttttttccc
    11641 tgtagtataa atcatattgt catttgttat aaaaaatgta gcttcacttc ctgctgtcac
    11701 aacagatttt acattctcaa tatttatttt tgtaaatgta cttatacttc cttcagtaaa
    11761 cccaaaatag ccatttacaa gtccacatac atagcatgta ttatcatttt taacaatgat
    11821 tgatacatca ttacatagat atacatctct aacaccattt ataggtatac gtgtaaatat
    11881 attcctactt gtagtatcac ccaatcctaa ctgtcctttt ccattattac ctgctccata
    11941 taaaagacca tcattagtta ataatagaga tgtgtttcca tatacagcta catattttac
    12001 attttcaaca tttattttag taaatatatt tctatttaca ttatctccta ttcctaactg
    12061 accagaatta tttaaaccaa cagcataaca ggtattatca ttttttatcg caaatgcatg
    12121 tttactattg tcagtaaatc tatttacatt tataaatttt atattatcaa tatttatttt
    12181 agtaaatacc tttctattta tctcatctcc cactcctaat tgacctacat catttaaacc
    12241 aacactgtaa agcatattat tattagttaa taaatatgta gactttgaat ggctatatat
    12301 ttcttgtata ttagaagata tttcactact gctattcttt ttattgtaat ttatcttatt
    12361 atttaaattt aaactactat taactgatat accatgcgta cccttagagt tatatccaca
    12421 tataaataag ctcttatcag ttaatattgc aaatctagtt ttatctctac ttaaatcata
    12481 aggaggtata aatatatttt ttatattttt cattaccttt ttataattag tggcattata
    12541 tccatcttgt gtatctgaaa catctagttc ttttgcactt tcagatatat tcccaaaacc
    12601 atatagcgtg ttagaagata aatacaatag agctttatat aaaaatatgg ctttatcaga
    12661 ggtaatagga cctgaattta tattagatgc tgcaatatta ttagatgatt gattgttaag
    12721 ataattcctt ccatattgat gtatataaga tgaacctcca gaaataagta aattatctgg
    12781 attgacattt tttaaattac ttgacgattc cccattatat aaataagcaa aagttgtaga
    12841 agatgatgat tgtgtacatc ttgtaggata ataattatat atataagaat caagatatcc
    12901 tgcatatgaa attccctgat attgaactct atagcttgaa tcagttggtt ttgtccatga
    12961 agtaccatgg tatccagtta gaaaagatcc aatgtttgta caaaatacag ttacatactc
    13021 atatatgata atattattaa ttttagtaat attattcgtg catgaaactc tactataact
    13081 tgaccctgat atatttatat aataccaatc acttaataaa cctagattta cattagatcc
    13141 atcagaagta ctaaaaccta acaggtaatt tgtatttata ccagttgcat ataccttttt
    13201 ttcatcaact acaataaatg ttgcattata tgaactataa gcatcagtta cattactaag
    13261 gctaatctca gtatattgac ttattaatgc attactaagt ccattaacat aacttgaact
    13321 ctttccagaa gtgtatattt gcttattatt agttacaatt aatgaaaacc agtttgataa
    13381 atcagtattt ataacaactt tttttacatt atctatagat attttcttaa atacatctga
    13441 attagaagga agaacacctg ctattgagtt actatttggt ccagaataat aacaagtatc
    13501 atcattttta atgtagaata ttcctccaga aatatttttt gatgctacta tagactttac
    13561 attagagata cttaattttg tcaactcatt taattgactt ggtaagtcag aacccattcc
    13621 tttgtatcta ttatttccta gaccatatac atctccattc cctaaaataa ccagagtaca
    13681 ataaggacta aaaacgacat ctcttacaga atgtatatct agtggaagtt caatatcctt
    13741 tatttccatt tcttctttta ataaagttaa tttatcctca gatttaatta aatttatata
    13801 attactataa ctatattttt cactatccat tttcttaaat tctacaatgt gcatatcatc
    13861 aaaaaacgaa cctaattgtc cataagtatt atctcctgta caatatgaat cattatttga
    13921 atcaattagt atgctatgtg ataaaccaat gaaaatattc ttagctacaa tattttttaa
    13981 atctataaat gaattatcta tattctcaga gtaatttgct atacctaact gatatatatc
    14041 attatttcca gcaaccttta aagtaccttt gttatctata agcatagtgt taccatgacc
    14101 acatgcaata gtttttatat ttacttcttc tatcttagta aatagtgatg attgaagtgc
    14161 atttccaaaa catttttcct ttccacaacc atatatatca ttattatacg tcattaacac
    14221 agtgtgttca gaaccacaag ctatagtctt aacatcatta atagaaacta aactaaataa
    14281 atctctatta ttcttatctc ctaaacctag ttgaccttga ttattaaaac cagtagaata
    14341 gcatttattg tcattcttta ttataaaagc tgatttatta tatgaaaata cagcctttac
    14401 attgtcaaca tctacttttg taaatgtatt tacatttgca gatgctccat tagttcctag
    14461 acctaattga ccatacaaat tatttccagc aacatataaa gttccatcat tgtataaaag
    14521 atatgttgat tgaagtccac atgcaatatc ctttacgcct tttatttctg gaaccttctc
    14581 aaatgtagaa actgttttcc ctactttttc taatcccaat cccatttgac catctgtatt
    14641 tatacctgcg acaagtaatt ctccatctga agtaagtata actgcatgag attctccaca
    14701 tgcaactttt ttaatattgg tagatatatt tggtattttc gtaaataatt ttcttgttgt
    14761 attgtcttct cccaatccaa gttgaaagtt actattatct ccacatccat acaagatatt
    14821 attatttttt agaccaaatg tatagttaca acatccagta aaaaaattaa taaagtcata
    14881 attatcatac ccttcatcaa attcatcaac taccatattt ttatcgccaa agaaattacc
    14941 tctttgaagt agtttagttc ttttcattat aaaaacctcc taattatctt atttcatacg
    15001 aatatttatt taaagttata tctgtctgac caattgaata tgccctatta gattttgttt
    15061 caacaaagtt attatccatt tcatgtatat atctaactct atttgcatta aatggtagca
    15121 ccacattctt aaatgtaaca ctaccaagtt ctccagttat acactttcta tttaaatcta
    15181 acttaaggcc ttctatagct attatgtttt tagcattagt taaggtatca taccaatatc
    15241 cagcttcatc tacatctatt tctgactcta attctaagta tattaagata tctaatactt
    15301 tttcagataa tttattgtaa ctatttttta atacagatag ttcagtttgt atttgacctc
    15361 ctattacatc atcaatagat atatttttaa ttttttctac tacctcattt atagctcctg
    15421 ataagttttt agcttctgta gataagagtt cttcgccaat ttttactttt atttctttta
    15481 tatttttatc aaacttatca tctaaatctt gaatatcctt ttgagttaca aaaaccaacg
    15541 tcgggtctac ttctaatgta acatttgaag tattggacaa ttgtaaaatc atttttacaa
    15601 ccaaatcttt aatactgcca tcttcagcac gtggcttata cgtctctgga tacttaccta
    15661 tagctattat attattctca ttatccagaa cagcagcttc tcttatcata aatccaccaa
    15721 ctgttcctgg taaaatagtc tctataacta tccaattagg atttttttca tcaattctta
    15781 gagaatttat atttccttcc caaactacat tctttaaagc agtttgttcc tctgttggct
    15841 cataatattc tccattacta tctccaactt gaaacttggt taatccaaca agctctccta
    15901 gtgcagttgc atttgctatt ttttctctac caattttggt taatattata taaaattttt
    15961 cttccaatta taccacctcc gatttaggat aaactactac attttctaca cctgtatcat
    16021 ttgcaatagc tatattaatc ttacaattat tttcaatgca tttaatgctt aaaggataaa
    16081 cctttatttc ttctctactt atcattccag ctccacagtt tacaacagca ctacatttta
    16141 taattggttc aaagtatgga tgaactttta cctcttcact acataccata cctccaccac
    16201 aatagaccat acttttatct agtattattg gttctaaata gtgtaataag tgagcaggtt
    16261 ttactctttc aatcatatcg ctacaatcta aaagagtttt atagtcacag ttatttgcta
    16321 taaaactcaa tacgaatgta aattcatcac tataaacttt tatatctgtt tctgattttg
    16381 tatatgcctc acatatactt tttataactt caatagtact agtacctctg cttctcattt
    16441 tagcttttat attgctacgt cttgtgtcaa aatcaagttc ttttttttca atgcagagta
    16501 tgtcttccca catatctaat ccccatgttg cagtgtcaac aaataattgg ttaaaggtat
    16561 catcaatatc ttctttaaga atatttaatt ctgtatcgta tgcaccttgt atctcctcta
    16621 caatgtaatt tctatcaaat gatggtagtt tatcaattag cttcattgat tttccacctc
    16681 actaaaatta acagttgtta cactaggtat tttatcttca ttaatagtta tattatctgt
    16741 acttccattt ataagtaggt tacttaaatc gtgtacacca gtagtattta taagtattcc
    16801 cattactttt gtatagatta tctctcctct aatatctcta aagtatgtat ttatactttc
    16861 taggaaagat tcttttacat tgtctaatgt atatccatct tctagtttca ttactgcact
    16921 tatacttatt tctattggta atggtgtcac aactgttata gttggtccaa taggcttctc
    16981 ttcatctata tgttgctgac acctttcaat tgtttctgta tcaacagctt gattattttt
    17041 cccaaatatc aagaccttaa ctgttcccgg accatcccat cttggataaa ccttaacatt
    17101 atagactcca tctacttcca aagcccattc ttcatagtga gctttatttc cacttgtagc
    17161 ttgatttctt tggattttat aaaatctttc ttttagttct tcatctgtct ctatatctgt
    17221 accaccttca aaacttcttg tgttagtaat ctttgttact ccagatatat tatcaactag
    17281 tttaaattca caatttgtag gtaaattata tttcttacca acttccagag cttgaactgg
    17341 gctattgtca ccttcttcac taccaatagt tacatctttt attactacaa atagtaaatc
    17401 tctatatgat attattgtgc cattatttat tacagtacct ttctctccaa taaattcaac
    17461 ttctccattt gactctgtac ctaactttct atatacacca aattcattga ctcttttatc
    17521 aagaaactgg ttatatgtgt cttgtataaa agccatttta tgcatctttg aaagttctag
    17581 atatatcttc gaaagttcta gattatttcc agataccatg ttgtttagaa aagaaccttc
    17641 tcctttataa atatcaagat taatattttc aagagttcta ttttttatta cttcatatgt
    17701 ctggtcacta tacatttatt tccacctccc catagatagt ggatactttt acatttgcac
    17761 tcaatatgtc tctgttaaag ttagcacttt ttacgtttac ttctaatata tatggattta
    17821 ttagaagggc ctcttttatg aatctactag cttcactttc tgtaagacct tttgtatatt
    17881 tttgtcctat tagttctgaa agctctgtcc catattctaa agagtatatc tcatgttcat
    17941 atctatttgt ctttatacac ttgtatatcc aaacttttaa ggcttctttt ttttcaatta
    18001 ttttaaagtc acctttttct aaaataggtt catctttttc aaaatcccat gccacttcac
    18061 gaaagattgg caattcttct gttttaggta agatataatc ctctgggaca cctataaaag
    18121 gaaatattgt actcattata agctcaccaa cttacttaca acagcaaatt tttcgcctat
    18181 tctaaacatt attaccatat ccccagattc aaagttatct ataaaaggat tttttacttc
    18241 atgctggtgt tcttgatttg tttctgtgtc aaatgtttct agctgtctat caatcatcca
    18301 actatctatc aaaatatctt ctttttctaa tatgatgtta tttgtctcta tttttaaatc
    18361 tggtaattta cttttaattt ttccaataaa aaaagaaggt tgattatgat actttccttc
    18421 ttctcttatt attcctataa attcatttat tggattagcc attatatcac catcctttta
    18481 taatactctc ctagctgtgt taaaatctct tctactactt aacttactta ttttaactac
    18541 atcacctttt tgtggagcat gtaaaaatta tccattacct atatataatc caacatggct
    18601 gactggatta tgaaaaaata ctaaatctcc tgcttgaaga tttttttgtt ctactttctt
    18661 gcctttttta gattgttgat ttgacgttct tggcaaacta acattaactt ttttaaaaca
    18721 atagaccatt aaaccagaac agtcaaaact acttggtcca tttccacccc atttataagg
    18781 tttacctaga tgtttttttg cttctgatat tactatttta gctttatcag atacattacc
    18841 agaataacca ccaataataa tttttccttt tcttcttcca aatttactag cttcttcaac
    18901 atttccaaat aaaatatcta tatggtatgt tccattcttt tctatagtta ttgcaggtcc
    18961 tacgtcattt actttataga ctccatcacg accagaaact cctgtaatta cctgtatttc
    19021 atctccatag tttaataaag gatgtttatt taaaaactct tttgtataat aagtttgctc
    19081 atatttacca accataggag cagcgcaagt ttttttagat gggtcaagtt ttttctttct
    19141 acaatctgta tctccacctt cttcttttct aggacagtaa gctgtaaatt ctgctgtaaa
    19201 ctcttttcct cctgcataat cttctccccc taaattactt tcttccttag gttcatcctg
    19261 tcctgctgac ttttcatcca taagattttg aaaattaagt tcaagctcaa tttgatattc
    19321 tccattttgc caagtatgtt tgtctgtatc tatataaaat agtcctacaa gctttgtata
    19381 agaatcttta acttttactc ctctaccagt tatacaactt acatctccat aacctttaag
    19441 agaacagctt ttttctatcc cattaaactc gctatcaata tctacatctt gattttcttg
    19501 ttgctgaatt actttttgca ttattacatt tacttcctta aaaatttcat tatctatttt
    19561 ttcgctaatc ttgcttccat actggtctac cactattact ttatttttta cattttccat
    19621 gctctccgaa aaggtggtat ttataatatt aaatccctct tcaaacataa cacttaatgt
    19681 aacagttcct ttttcaataa cattaaactt atctaaattg gcctctatca tatacttttt
    19741 ctttgtcttt ttacttgctt ctgtatatgc actcattatt gtgtcataac catttactcc
    19801 tataaacatc tttgtatact tgacattggt ctttgctatt gttccaactg aaagcctatt
    19861 ttcagcaaat acttgctttg ctatgtcttc aactaactta tctttaaaat tgtatgacac
    19921 ttcactttgt gtaagtaaaa atcccatatc tttagataca aaactaattt cattactgct
    19981 tgaatcttta gacctattaa ttatcattcc tctaaagagt tctttctcat ctacatagaa
    20041 acagactgta ctagctattg gtatatcgat ttgttggaaa tttacatcaa atgatgattg
    20101 tattattgaa aactctagtg tccttgatgg agatttataa tcacctgacc atgatacctt
    20161 gtctactatg tcagttatgt catatatact tccgtttttt atgtgtaccc aaatcttaat
    20221 aataaaaaat cacctgcctt ttcatttaat tctatcagca gttatttata acttattatc
    20281 tataatagtt attgttttgg agttactttt ggtatcttta ttcttttata ctcttttaaa
    20341 tctaatgtaa aatatacatc tcgagtccca tctctttctt cataactaaa cccttcaatt
    20401 atgacttcca tatttatatt tgtttccgta attgtaaatc ttaatataaa accttccttc
    20461 atccattttt ctatcttatt tacacaatca tatggtgatg gaaaacctgt atagtcacag
    20521 aaagtatagt ctttatttgg aaaaaaactt gatattgaaa tgcttttaag agctacacca
    20581 ccaaaagttg ctatttcacc tattttgagt atactagaag agtttacagc agcttttcca
    20641 ttaatactaa aggaagatgg aaatactgga aatctaaatc tatcttcagc ttgtctaagc
    20701 cacatttcca ttataagaca ccccctaact gtgccaattg gatacttgca actaattttg
    20761 atgttatctt ttctatatca gcttcttctc taatcactat tgtatcagct aacttagcta
    20821 tgtttatatt tccaccattt tttctgcttc tatattgatt tgcttcttgt ttagttaaaa
    20881 ccatttcacc ttcatggagt ctggtttgat agtcattata aggaacatag ttcaatccaa
    20941 atgcattatt acctttctta cctgaataag ctttaccaga atcaccagcc tttgttccag
    21001 catatggatt atctttgtaa ttttttttac tcttttccac tagatttaaa actgttcctg
    21061 tgatgcttcc attttttatc ttattaatca tagtttgcca ccacttgcat atatctttaa
    21121 ctattttaca tactttatct aatatgttaa aaaaagctcc caatattggc tctataattt
    21181 tccatccagt ttccaacaga ggtcctaagg tctcccatac agttttccaa ataacaccca
    21241 gtgtctctat aaaattgttt atttgaggtg catattcacc taaaaaagta aaaattcttg
    21301 tgataatagg actaacagct tcaactacag gtccgattag ttggaatgct gtagttacta
    21361 ttgtaccaaa tatacttatt aatccttgtg caattggtga tttattgttt atactatcta
    21421 gtaaattatt tactaagttt actactggaa gtaatgaatc tattaatgct gtaaaatcga
    21481 tattttgtaa tagtgtactt cctgcgttaa caattgcttt tggtgcattc cctatcattg
    21541 ttgctgcatg attttcaaaa cgttcttgct tattttcaac catcatttta cgcttttcag
    21601 cctcattaga aattcgatat tgagtagaat cagaaaactc ttttccattt tttacacttc
    21661 tagattgcat ctggtcatat ttgttgccaa taattatctt accattcatt acattgccta
    21721 ttttaccagc cattttttca gataagccag ctaaattgcc taatccactt tctataaaag
    21781 tttttatttt ctttagtcct tgacctaaaa tatcatcaaa aggtttaaca atactcttca
    21841 agccattgtt aatgcttgtt tgagctcctg atattatacc tggtattgta ttactcattt
    21901 ctttagctaa ccccatctgc tttacactta tgccttccaa tgcttcatca aaagttttat
    21961 aattttctcc taccagagta ttaagagatt ccatgtttcc ttttgctgca cttaagaaag
    22021 ctgctgatac atcttgttca ctacttgtat tcatatttaa agctctaaca tctaccatag
    22081 cttgagttat atctttttct tttttgccac cagtaatatt actaatcatt ttagctttag
    22141 ttcccattgc aacaacatct tcagggtcaa aaggcgtatc atttgccatt cttactaaac
    22201 tgccataata atcatcaaca tctatttgat taacttttcc agtatttctt ttcattacac
    22261 tttctagaac aactttctga ttttgttcct gaccaagtaa atcaacaccc tttttcacag
    22321 cgacacctat agtagcacca tttgtcaaat cagatatctt cccttttatc gaaccaagta
    22381 ttccacttgc catatccttt acagctattg caccactcca taccttacct gcaaataatc
    22441 tcaatttggg aaatacttta cttataatgc cacttgccat atcttttata gctattacac
    22501 cactccaaaa agtattttta aacaacatta acttaggcaa aattctaccc atagcactac
    22561 tcaccatatc tttaagatta atacttcctt gataaggacg tttctctact tgttttaatt
    22621 ttcccaagat actacttatc actctcttag tcatatcttt tgcttttatt acgatattcc
    22681 aaatcttgcc accaatcttt tttaaatttc caagtaattt atttttagtt ttttctactt
    22741 catcactttt agctttaaat attaatataa tcataatatt tgaattaaac tcatttttct
    22801 tttgaatcaa cttattaaaa taatcaataa tcatagctat agttattgct agtctcataa
    22861 tacttccatt taaacttcca ataactcctg aaacagcatt tatgctagaa cttaattttg
    22921 tcatagttga acttacacta ctagcagtag tttgaacttt tcttaacgag gaatttaaac
    22981 tgtttactct attacttgca cttccaatag aattttttat gctactagcc attacattag
    23041 tggaaagtga aacagccaca gttgctctcc taaaattatc agttactgat ataatttgat
    23101 tcatttttgg tgtgtaatta tctgtaaggt acatatttat accttcttct ctcacatttc
    23161 ccattacata ccacctcctg tcaaaggaca cactccatat ccatgtttca ttttttcaac
    23221 ttcttcctca atttctaagg cgaaaaaagc ttcaataagc tttaattcgc ctttatccat
    23281 tgcataaaaa agggacggtc ttataccttt tttcttccaa taatagtaca ttattgtagt
    23341 cctggtatcc gtccctatta gttttttacc tcttctatca ctgcattttt accataaccc
    23401 ataacatctt gaagtattct atataagttg gtaatttcac ctggtaataa caattttctt
    23461 gctaattctt ttggtgttgg agccttaaat ttatccatta gctccttatt tttaaataaa
    23521 agctttccat ccaaatcaca aacaccttct ataattgtaa gcatctgcat ctccggtaag
    23581 tctatatccg ttttcatatt ttcatcaatt ttaagacaag tattttgtat ctcttcatct
    23641 ttacttattg ttaaagctct acacattact ttaaattcct gcccaaatat agttgataat
    23701 ctcttaagtt caactatagt acttggtctt tctaagtttt ctgtatctgc atttaaaagt
    23761 aaatctacta tgtttatatt ttttaataat ccattttcac tcattattat ttgctccttt
    23821 atcaatcaaa ttcaatcaac taactttttt acttattaac tatctgaatt tattaagtat
    23881 atatttacta aatatcagta taaaaccaaa tataaaacta aatataaaac taaattatat
    23941 caagaaaatc atactcagta aatgtgaaag gtgcttctat ctctcctttt acgccaacct
    24001 cccaatcagc taaagttaaa tcatcaaatg ctatattctt tactgctatt ctttctgctc
    24061 cataagaatc tgggtcattt aatttagata tagcaacaaa tctaggttca gaacctctct
    24121 ttatcttttc tccaattaac ttgtgcattc ttgaactaac atggtataga gttattgagc
    24181 cttttccttt atatcccata tactttgtat cagtacccat ttgacctgct attataatat
    24241 cttcttttgt aaattccatc tttgcttgaa acttctttac ttctgctact ttgtttccat
    24301 caagccaaag ttctccccaa gtaccactca ttacatttct agcttccata tttgccatat
    24361 taataatcct cctatatttc tattgataaa tctatatctt ccatagcatc aagtactttt
    24421 atttttgctt ttaaaaatac tttagaacct gtgttagctt cttttatttc ttgtaatgtc
    24481 atataagata aatctactcc ttttgatttt aaatacgatt tttgtgcttc aaaatctatt
    24541 tcaacagtag aatcagattc tataagtgct gatttttcta attcttctaa ataacttttt
    24601 atagctacta tcaataaaca tttgttgtca taactgttag taacctttcc tatatagtca
    24661 tctattatca cctttcttat gtcactatgt ataatatcta aagtgtcaac tatttttatt
    24721 ttctggaaca tttctccttt ttctgctgtt aattcagtta aagaatttac tcctctagca
    24781 attcttatag ctcctgcttc tttaataagt ataagctctc ctttattaac ctttgattct
    24841 gcatcaactt tcgtcatctt aggtatatcg actacatcac taagtttagt ataagttact
    24901 gattgactta aaggtgtacc tgctataagt ccagccaccc tacttgtaaa ctcatcaaca
    24961 ctgtatttct tttctccaac taacacatct tctgtagtaa aattaattat cccttcatga
    25021 tttcctacaa cttttcctaa tacagcttta accttaacct tatctatatc tctaagttta
    25081 attatccaat tttttatagc agtcttatca gcttctactg cttttggcat acatagataa
    25141 ttaaattcct tagtctctaa aaaatctaat gcagtttgaa tatctgcttc tccttctatt
    25201 acataaacta ataatttatt tggagtgtta acatttccca tcaaggctaa atttatatat
    25261 tctttatttt cagcagataa atcaactggt atatcctctt tttcatgtat ttcattaaga
    25321 cctagtgcct tagcatcttt aagcaccatt gcaattattc ctctagctga acgttcttta
    25381 acagttgtag ctagctcctt aaatgatatg ttgatacttg gtaatcctat agccatacta
    25441 tcactctcct aaatttaaat tgatttcttc catattttca tatactacat cttcttcata
    25501 tacactatcc agatagttta gtgtaagtgt aaagttcaat acataaattg actcttcttt
    25561 tttaatactt tgttctagct ttgttatatt taattttctg tctgttacct ttatatttct
    25621 gttaaatagc ttatttaatt catctgaaac ttcatacatt ctaatactct ttgactgtgg
    25681 ttttggtaag tattttatag agataacaat atctttgtta ttcgtattta acattacagg
    25741 tgtacaaata actgatagta tacttacaaa aaaacaagac ccttcagagc cttgcacact
    25801 ttcatcatca ataaaaatac tttcagtaaa gttttttttg agaatctcaa taattgtttc
    25861 taatatttcc ttgtatttaa gcaaataatc accacgctat cactaaatat tcttgcttaa
    25921 tctgataatc ttactataaa tgatataagt catttttaaa tatatatctc atttattcct
    25981 gtcaagtatc ttctatattt ttaggtaata ttattctttt caatctatca cctccttcaa
    26041 tatggatttc tttcacaata acattatctc acatatttta ttgcctattg ttgcaatggt
    26101 gtatataaag tgttgtaaaa ttgtcctatt ttttataata tctcactcaa tttaaatatc
    26161 atctttttct ttaaattgta gcaagatgtt ctatctaagt gtagtttcat agaaatatat
    26221 gtcatattgt tttttgtctt actattataa aaaagattaa aaaattccat ttctatatca
    26281 tttagacacg ttagagcatt ttctatcttc tctttttcta tttctatatc ttctttttcc
    26341 atttttaatt ttcttaatct ttcctctctt tttaacacct cattttccac agaagagctt
    26401 atgttatatg tgttactagt cttttctgta tataccattg ctccacatcc agagtattca
    26461 ttctcacatc tttttatgtc caagttaata ttctttatct ttatatccaa atacttataa
    26521 ttatgtaatc ttttatctgt ttctttaaat aatatatctt ttttgttttc catagtactt
    26581 ctcctttttt atatacttat tattagcttc aaatttacaa taatctcttt agtgcacagt
    26641 tttttactaa tctcacaact ttcaagaaca tattgttcta tgactgtata aaaaaaactt
    26701 cttctatatt ttgttctaaa atactagaaa tcttcttagc cataagaagt ccaggatttt
    26761 ttatgcctct ttctatttga gatatataat catttgtaat tccagccttt ctggcaagtt
    26821 gaatttgtgt catacctttg cattttctat aatatttcaa gttattttta aacattgttt
    26881 tatacctcca ttactattaa gaatattttg ttcttcctca cttagattat atagaattat
    26941 ttgttcttta tcaatatatt ttagaaaaaa atattctttt atattttatt agttttattt
    27001 tttagaacaa tttgttataa tctatacata aggtggtgtt ataatgtttg ccaaaagatt
    27061 aagagaactt agaaaggaat ttggattgac tcaaagagaa cttggagaaa aagtaggcgt
    27121 ttcccaaaga gtcttagggt actatgaaac agaaaacaga tttcctgatg aacacatttt
    27181 aaataaattg gctgatgtat ttaatgtatc agttgattac cttcttggaa gaaccttagt
    27241 taaggaaaat attgatacag tagttgcaca tagaaaaaat ccacatgaag agttacctga
    27301 agaagcacaa gaacaactta atgattatat agaattttta ctaaataaat ataaaaaaaa
    27361 ataaaccata tgatgagcag tctatttttc tgctctttta atataattta aaggcaaaca
    27421 tacattctat tttaggggga tttttatgaa taatttggat aagctttttg aattagcttc
    27481 tcaagaaggg ataataattc attataccac ttatattgca ggtgatttag aaggactata
    27541 tataaacaaa catggtatta aaattatatc attactcagt aatttaaaac aaaactcaaa
    27601 aaaattaaca tccatcttag ctgaagaact tggacatcat ttcactagcc ttggatacta
    27661 tgtatcctct tataacgatt attatacaaa aattattata gataagtgcg aaaataaagc
    27721 cttaaaatgg gcttgtgagt tcttaattac tgaagaagat ataataaaca taattaactc
    27781 tggcatcacc tgcgtttatg aaatggcaga catacttaat gtggatatta ctttctttca
    27841 aaaaagatta gaatttttat cattaaaaaa acagtcttta caacttggaa ataataaata
    27901 tttaatatta actaatttgc cgtatttcta catatttgat ccaatttcat aataaaaata
    27961 gtagtaattt taactggctt aacagtaaaa tctatatatt tattttaaca aattttggtt
    28021 tctataaatt caatattaat atataataat gatatcatat tatttaaaat tctaaaaaca
    28081 aatatatgct tttgttaaga aaggttgttt gatgaatgta taaagaaaag ttgtttgata
    28141 attattttaa gttcttagca ctattatttt ggccaattat gtggtataag tggatagtaa
    28201 tttcaaatgg tactcttgaa aatatgttat ttaccatata tgcaatcgtc gcaattgttt
    28261 tcatcatttt atattcggtg tttatgataa aatataaaga cattacacaa atagatttct
    28321 tttacaggat aagtactcta cttgctttta tatttacact ttttagcttt ttaatatacc
    28381 caaagagttt atttttcttg tatttaaaga ttatttttac tggaat
//
LOCUS       DAEMIK010000036        28207 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEMIK010000036 DAEMIK010000000
VERSION     DAEMIK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.036"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            37..822
                     /locus_tag="KPK87_001350"
     CDS             37..822
                     /locus_tag="KPK87_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2537515.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRIIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            991..2556
                     /locus_tag="KPK87_001351"
     CDS             991..2556
                     /locus_tag="KPK87_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2537516.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            2570..3901
                     /locus_tag="KPK87_001352"
     CDS             2570..3901
                     /locus_tag="KPK87_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2537517.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            3915..4403
                     /locus_tag="KPK87_001353"
     CDS             3915..4403
                     /locus_tag="KPK87_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537518.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            4417..4884
                     /locus_tag="KPK87_001354"
     CDS             4417..4884
                     /locus_tag="KPK87_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2537519.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDIQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            4887..6041
                     /locus_tag="KPK87_001355"
     CDS             4887..6041
                     /locus_tag="KPK87_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2537520.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            6357..7109
                     /locus_tag="KPK87_001356"
     CDS             6357..7109
                     /locus_tag="KPK87_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537521.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNELYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            7110..9017
                     /locus_tag="KPK87_001357"
     CDS             7110..9017
                     /locus_tag="KPK87_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2537522.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            9169..9849
                     /locus_tag="KPK87_001358"
     CDS             9169..9849
                     /locus_tag="KPK87_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537523.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            9843..10862
                     /locus_tag="KPK87_001359"
                     /pseudo
     CDS             9843..10862
                     /locus_tag="KPK87_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
     gene            11293..12453
                     /locus_tag="KPK87_001360"
     CDS             11293..12453
                     /locus_tag="KPK87_001360"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG2537524.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLIQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            12455..12862
                     /locus_tag="KPK87_001361"
     CDS             12455..12862
                     /locus_tag="KPK87_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537525.1"
                     /translation="MSNNKKKKRKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNI
                     PTISFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVET
                     KRGFSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            13124..13432
                     /locus_tag="KPK87_001362"
     CDS             13124..13432
                     /locus_tag="KPK87_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2537526.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            13521..14867
                     /locus_tag="KPK87_001363"
     CDS             13521..14867
                     /locus_tag="KPK87_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2537527.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            15100..16197
                     /locus_tag="KPK87_001364"
     CDS             15100..16197
                     /locus_tag="KPK87_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG2537528.1"
                     /translation="MGSLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWTVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            16296..17210
                     /gene="murQ"
                     /locus_tag="KPK87_001365"
     CDS             16296..17210
                     /gene="murQ"
                     /locus_tag="KPK87_001365"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG2537529.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            17320..18239
                     /locus_tag="KPK87_001366"
                     /pseudo
     CDS             17320..18239
                     /locus_tag="KPK87_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
     gene            18256..19107
                     /locus_tag="KPK87_001367"
     CDS             18256..19107
                     /locus_tag="KPK87_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2537530.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            19719..20780
                     /locus_tag="KPK87_001368"
     CDS             19719..20780
                     /locus_tag="KPK87_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2537531.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(20896..21870)
                     /locus_tag="KPK87_001369"
     CDS             complement(20896..21870)
                     /locus_tag="KPK87_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537532.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            22326..23207
                     /locus_tag="KPK87_001370"
     CDS             22326..23207
                     /locus_tag="KPK87_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG2537533.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            23589..24392
                     /locus_tag="KPK87_001371"
     CDS             23589..24392
                     /locus_tag="KPK87_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537534.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            24404..25534
                     /locus_tag="KPK87_001372"
     CDS             24404..25534
                     /locus_tag="KPK87_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2537535.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            25538..26947
                     /locus_tag="KPK87_001373"
     CDS             25538..26947
                     /locus_tag="KPK87_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537536.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            27158..27667
                     /locus_tag="KPK87_001374"
     CDS             27158..27667
                     /locus_tag="KPK87_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG2537537.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT        11092 a         2877 c         4894 g         9344 t
ORIGIN      
        1 tttatgacga tttttaatta aagggggtaa tagtttatga gattagaaga aatgattttt
       61 ttaaattata ataaattgaa tgatacagat ttattaatat ggaaatatat aactgcaaat
      121 aaaaaacatt gtagtaagat gtcaataaat gaacttgcta tgaaatgtaa tgtgtctcgt
      181 gcaactatta caagatttgt taaaaaatta gatttaaaag gtttcagtga gtttaaagta
      241 ttattaagct gggaatcaga aaaggttcat gtcatagaaa atcagtcatt tgatattgca
      301 tgtaattcaa taattaagta tgttgaagat caaaaaaata aagattatga tgcattgtgt
      361 aaacttcttt atgaatcaag aataatatac gcttatggtt caggtgatat tcaaaatgtg
      421 gttgcaaaac agataaagag aatgttctta tcatgtcaag aaataatata tgattttgag
      481 ggaagaacat ttgatgattc tttttacaat attgtcagta aaaatgatgt tatgattatt
      541 atatccctaa gtggtgagaa tgagaataca cttaatattg ctagaagatt aaaattaata
      601 ggaactaaaa taatttcaat tacagagttt aaaaataata cattgactga tttaagtgat
      661 gaatcaattt atatttcagc gactaatatc tcatttttac aaatgcaccc tagttataaa
      721 ttgacaatgt tatattttat attgattgag ttattattta ttaaatatag tatttataaa
      781 aggcatagaa tgattacaga agggattgat tgcaaattgt agtcattctt ttttttatga
      841 gaatttgaac ataaaaatgt gcaaaaactt atttgaatgt gaataagctc cgaatgtacc
      901 ctaaagattg tatatattta ttaacctgtt aaaataatag atatctaagg ccaaagataa
      961 actaaaaata tgaggagggg agtaatttat atgatgaaga aaatacaacg ttttggaggg
     1021 gcaatgttta caccaacact attatttgct tttgctggga taatggttgg attctcaatt
     1081 gtatttcaaa atcagtcaat tatgggttca cttgctacac cagaaaatat ttggtaccaa
     1141 ttttggggtg taatatctag tggagcttgg atggtattta atcaattacc attattattt
     1201 gctatttctt taccaatagc acttgcaaaa aagcaacaag caagagcttg tatggaggct
     1261 ttagcaacgt atttgacttt taattatttt gtaggttcta tgttaagttt ttggggaaaa
     1321 agctttggag ttgatttcgc agctgaaata agtgctggaa gtggacttgt aagcattgct
     1381 ggtattaaaa cattagatac tggtatggtt ggtgctttgt taatatcagg tattgtcatt
     1441 tatattcata atagatttta tgataaagaa ctgccagatt ttattggatt atttagaggc
     1501 tcatcacttg tagttgcaat ttgtttcttt gttatgatac cagttgcatt attaacttgt
     1561 tttatttggc ctcatattca aaatgttatt agacatttgc aaacgttttt tataaatagt
     1621 ggaaatatag gagtttggtg ttatgcattc ttacaaaaga ttttaatacc aactggatta
     1681 catcattttg tatatgcacc aatttgttat gatagtgttg ttgttccagg tggtacatca
     1741 gtatattggg ctactcatat tcaggacttt caaacttcag ctaagactct aaaggaaatg
     1801 tatccaatcg gattttcatt atccggatta tcgaaggtgt ttggttcatt aggtgtattt
     1861 ggtgctttct atgtaactgc aaagccagaa aaaaagaaaa aagtattagg tttaatgatt
     1921 cctgctactc ttactgcagt attaactgga attactgagc cgttagaatt tacattttta
     1981 tttgtagcac cacttttatt cctagtacat gcatttttag atgcatgctt gcaaacgatt
     2041 tcgtttgccc taggagttgt tggtgatttt ggtggaggaa ttattaactg ggttgtatta
     2101 aattggcttc ctcttggaat gtatcactgg aaggtatatg ttgttcaagt tgttgtaggg
     2161 attatatttt catttatctg gttctttgta tttactttct taattaagaa gtttgatatg
     2221 aaaactcctg gtagagagga agatagtgaa gaaacaaaac tatatactaa aaatgaatac
     2281 ttagaaacta aagatgaaaa aggaaataaa ttgagtaaag cctctcaaca agcttctgaa
     2341 tacataaaat tagttggtgg agcagaaaat gttgtagatg ttacaaactg tgctacgagg
     2401 ttgagattaa ccttgaaaga tgactcaatt atttctaaag aagaagattt taaggcagta
     2461 ggtgcacatg gtttagtgca taatggaaaa gctattcaga ttattattgg gctttcagta
     2521 ccaagtgtaa gagaagaatt tgaaaattta ttataaagga gaaggaaaga tggaaagaaa
     2581 aagacagagg atactaattg ctggtggggg tagtacttat actgctggaa ttgtaacaat
     2641 gttaattgaa agtgttgcta aatttcctat tgaatcaatt aaattatatg ataataatga
     2701 tgagagacaa agaaaagttg ctgaagcatg tgcaataatt gttagagaga aaaatccaga
     2761 aattaaattt agttatacaa caaatccaga agaagcattt actgatattg attttgtaat
     2821 ggcacaaatt cgtgttggtt tatatgcttt acgtgaacaa gatgaaaaaa ttccactaaa
     2881 atatggggtt gttggtcaag aaacttgtgg ggcaggtgga attgcctatg gattaagaac
     2941 aataggtcca ataatcgaaa tgattgatta tatggaaaaa tattctccaa atgcttggat
     3001 gcttaattat tcaaatccag cagctatagt tgcagaagca tgtcgtgtat taagaccaaa
     3061 ttcaagaatt attaatattt gtgatatgcc agtatgttta gaggaaatat ttgctagagt
     3121 tttaggttta aattctcgta aagactttga tgttagatat tatggattga atcatttcgg
     3181 gtggtggaca agtattaagg acaaagaagg aaatgattta atgcctaaat tacaagaata
     3241 ttgtgctaaa aaaggatatg aggaatttac acctcaaggt caacacaaag aatctagttg
     3301 gttagaaact atgagagcag caaaggattt attggaaata gaaccgacta cattacctaa
     3361 tacttattta aaatattatt taatggctga tgaaactgtt gaacatgcta atccaaatta
     3421 tacaagagca aatgaaatta tggatagaag agaaaaggat acattcgaag aatgtgaaag
     3481 aattataaaa aatggcacag caagagatac atggtttgat gcaagtgaac attctcaatt
     3541 tattgttgaa ttagcttgtg cattagcatt taatactcag gaaagatttt tactgattgt
     3601 tcctaataat ggagctattg aaaattttgc agatgatgca atggttgaaa ttccttgcct
     3661 tgttggaaaa gatatggttg aacctatgtc tataggtaaa attccaactt tccaaaaagg
     3721 tttaatggaa caacaagttg cttctgaaaa attagctgta gaagcatgga tagaaaaaag
     3781 ttatcaaaaa ttatggcagt cgtttactat gagtaaaact gttccaagtg caaaagtagc
     3841 aaaagaaatc ttagatgaaa tgattgtggc taataaggat ttctggccaa cttttaaata
     3901 aggagaacaa aaacatgata acaaataaaa taaaaaatat cacagcagta ttaaaagatg
     3961 aaaatatatt tctttcagtg gttatggttt gttttctaat attacctgtt ttatatatag
     4021 tgtatttaga attgacatca ttatttacaa atatttcatt ttcacaactt ttaaacaatg
     4081 atgttaatgc aagtttaaat ttaatctcta actttagtgc agtatacagt gcatatgtta
     4141 tttacaatat aaaaaagaat aaagagataa aagaaaacat gattgctttc tatttagtct
     4201 tattagggca gatattccaa atttacacaa taactattgt tatcatgttt acctatattt
     4261 ataaatttgt tggaattaaa caaatcataa gcagtattaa agaactacaa tggggaaaga
     4321 gctataaggc tcttttttcc tcaattgcag tatgcttaat atcgttattt gtaatgataa
     4381 ttagaataaa gttaattatg tagaggagtg tctataatgt tctttaaaag aaaaaaagaa
     4441 gcaattacaa ttaattccta cattgatgga aaaataatta atcttggaga aattcctgat
     4501 aaagtatttt caactaaggt aatgggagat ggatttgcta tgatagcaaa ggacaatatt
     4561 cttaaatcac cgactaaagg agtaataagt tttattgctc aaactaacca tgctatagga
     4621 attaaattgc aaaatgatat tcaaattctt attcatattg gattggatag tgcaacaaca
     4681 aaaaaagaaa actttaaatt agaagttaaa caaggtcaag aagttaatat gggggaaaag
     4741 ttagtagaaa ttgataataa gttacttgaa aatactttat atattccttt agttattgta
     4801 gagaatccta atacggtaaa atatcatctt tcaaacttaa atcaggaagg aaatagtggt
     4861 attaaagtta tcaattttga ttgattatga ttaataaagc gattaaattt aaatttatca
     4921 tattctattt tttctcatat attatttttg cctttgcttc aacaaaattc acacctttct
     4981 tatcaaaatt gggttactca gcgtttgaaa gaggaattat attatcgagt tatgcaataa
     5041 ccaatatatt atttcaatta ttatttggag tattagctga taagtatcaa acaatgaaaa
     5101 aaattgtttt aattagttta tcagtttatg gaattgcatc agttattatg ttttctactg
     5161 aatctgcaac ttttataata tacttattac ttgttgcttt atcaggagga ttattaaata
     5221 cttgctgtag tttatatgat acatggataa taggttgtgg agataatatt aataattatt
     5281 tgtcatttat taagactttt gggtcgattg gatgggctat tggaagtatg gtagcatctt
     5341 acttgattct tagattctca tattttggat tatctgtagc gattggttta attgtagtat
     5401 tacttattat aaatatacta ttattacctg atattgaaaa gattaataag aaaataaatg
     5461 taactaataa agatattatt gaattattaa aagataaaaa atatatctta ctagtatgta
     5521 ttttattctt attatactca atggtggtag caaataactg tactgtaata gataaaatga
     5581 tagctttaaa tgctacaaat tctcagatat ctttaaagtg gtctttacag tcattgttag
     5641 agataccaac atatctggct ggttcttatt tattaaaaaa atatagcagt ttaaacttat
     5701 taaaattaag tgctattatg ataacagttc agtttctatt atttgcttta acaaatgatt
     5761 caaatatcat tattatactg tgtgtttttc aagtgttttc tactccttta attttaatta
     5821 cttcaaaaag attaatattt gaagtttctc ctaaaaaact tagaagtagt agccaattaa
     5881 tagctttaag tatatttatg ggaggttcat ctctgattat tccaactgtt gctggttttt
     5941 tgagcattaa tattgggtat aattatacat taatggtatt aagtcttttt ggatgcttag
     6001 cttattgctt gatagtctta ttgaattatc ttcaggaata attgttttaa tatatagtag
     6061 taatatcact tgtttatagt atctgtattt gccacaatca atgtgtattt tggtttttta
     6121 tagagaagaa taattgtata gattagaggc ttcttaggag cttcttttct ttgtttatac
     6181 aaagaagtaa atcatgcttt tattttaaga tatttccatt ataattataa tagtcttcca
     6241 tatgttacaa aagtgtaatg ctaacagtta aaatgtaata tttacttaag gatttattta
     6301 tataaaacct ttaaaataaa tatatattaa aaaattagca actggaggct attattatga
     6361 aagaaatatt aaaaataaaa aatatatcaa aagattatgg aataaaagga tttaaaacaa
     6421 atgtattaaa aaatatatct cttacagtca atgaaggtga ttttatatca ataatgggtc
     6481 catctggtgc aggaaagaca acattgctaa atttaatgtc tacacttgac aaacaaactt
     6541 caggagaaat aatactagat ggaattaata tatcaaaagt aaaaaataat gaactatcaa
     6601 aattaagaag agaaaaaata gggtttatat ttcaagacta taatctatta gacaatatga
     6661 aactaatgga caatatagca cttcctctag cattaggaaa aaagaaaagt aaggagatag
     6721 aagctaaggt attttctata gctaaaaaat ttggattaga aaatcattta gataaatatc
     6781 catatcagtt atcaggagga caaaaacaaa gaggcgcagc agctaggtca ttaattacaa
     6841 atccaacagt catatttgct gatgagccaa ctggtgcttt agactctaaa tcagcatatg
     6901 aacttttaga atcattagaa aaaataaaca gagaaaataa cgctactatt ataatgatta
     6961 cacatgaccc tctaacagca agttattcaa atgaattata tatgataaat gatggaaata
     7021 taaagtgtaa attaaacaaa ggaaatagta gaaaagaatt ttatggaaaa attatggata
     7081 tgctagcctc tatgggaggt gagatgtaag tgagtatact tggaattgct tataataatt
     7141 ttaagagtaa tataagaact tatctagcat tttttatatc tatggttttt tcagtagttg
     7201 tgctaactaa ttttgaatta ttaagatatg gtgatgctat aaatgtgtta caagatgaaa
     7261 ataaaaaatt tacactatca gtgttagtag ctgtaataat gatactatca gtatttttat
     7321 ttttctttat atggtatgca acaaatatat tttttaagaa taggtctaaa gaaattggaa
     7381 tattgtcttt tatgggatta gatttatata ctataggaaa aatatatttt gtagaaaata
     7441 tgcttatagg aataagttct tgtataacag gaataattat agggataata acatctagat
     7501 tttttcaagt agtgataata aagttatctg gatttaatat aaaggtatct aatggactta
     7561 gtatagaagc tatactatat gctactttaa tctttatttc tatatttata ataatggctg
     7621 taaaaggttt tataacaata tgtagaagta gtgtaataaa tctaatcaat atatctaaaa
     7681 aacaagaaaa aataccaaag gtaggaatag gtatatatat attagctata gtatcaatta
     7741 tagtaatagg atatggctac tatctttcta ccaatataag aactggagat ataactagtg
     7801 tactacctgt tataggtata ataatagtag gtacttatgg attattcaaa tcagttatgc
     7861 ctgttatatt tgatattata atcaaaaata aaaaaatatt atttaatgga aataatataa
     7921 ttgcaataaa caatataaac taccgattaa ataaaaacta taaaacttat gctataatag
     7981 ctataattat aactacaaca atatcaactc ttggtgcttc agttgcgatg aagcgtatac
     8041 ataagaatgc ccaagaacaa agaaatgtgt acactattag catagtttca atggataaag
     8101 atgaagtgaa taaaaaacaa atagagaata taataaaaga tactaataag ataaagtact
     8161 cagtaaatcc agaactaaca attttaaaga aaacagatat aaaagaagct tatgatacaa
     8221 cagatactat aatgaaatat agtgattttt gtaatactct taaagtgaat ggcaatgata
     8281 aagaattaaa agagtatggt gaaagattag ttagtggaaa taatgtaata gagttaaaaa
     8341 gccctcaaac gatagcatca atagctacaa aagaagccaa gataaaactt gatggtcagt
     8401 tatataatat ttcaagggga gaagtaaaag ttccagtact agggacagga cttaatcaaa
     8461 gtattatagt ggtaaatgat gatacatatt ttaaactaag agataagggt caacaagtgt
     8521 atttttatgg tgcaaaggta tttcaagaag aaaatagtca agtaatgttc aacaatattg
     8581 aaaagaattt aagtttaaaa aatgtgtata ttcaaaatgg atataaagct caaggtgaga
     8641 gtcaatggat gaagtttgca tatgtagtgc ttgtgttttt attcttagtc tttgcaattg
     8701 tagctggaag tataatatat atgaaaatat atagtgatgc atatgaagat aaagagaaat
     8761 ataagatttt gttaaaaata ggtactacag aaaaagatat aaatagagct atacttaaag
     8821 aagtagctat tttttatact ttgccaatgt taagtgcaac tatatctagt tattttgcat
     8881 tgagattagc aggagattta ttgatgactg atttatttgg tatatatata ctaagtttag
     8941 tgatatgttt gataatattc ataatatatg gagcagtatc tgttaataag tttaagagtg
     9001 tagtgtataa aaattagttg atatattaat aaagagaaag gagattcttg taataagagt
     9061 ctccttgttg attaactaaa taatttgggt aataaattca tataaaatat gttatgatat
     9121 taataattca tagcatagtg aaatattagg ataatggaga atatacaaat gtatagaatt
     9181 ttattagtag aggacgatat tgatttatca aaagagattg cgttagcact tgaaaaatgg
     9241 ggatttaaag tagaattaat tgatgatttt gaggtagtat tagatgaatt tatagataaa
     9301 aaaccagatg tagtactttt agatgtaaat ttaccattat ataatggatt ttactggtgc
     9361 gaaaaaataa gagctatttc aaatgtacca ttaatatttt tatcatcaag agattctgat
     9421 atggatttaa taatgggtat aaataatgga gcagatgatt acattaccaa accattttct
     9481 atagaaatat tggttaccaa aataaatggt ataataagaa gagtctataa ttatagtgat
     9541 tctaatagta ttttgtattg tgaagatttg atgtttgatg tggggaaagg gattataaaa
     9601 cataagtata aggataaaag tatagagttg actaaaaacg aaataaaaat tttaacatta
     9661 ttattaaaaa ataagaatag ggttgtatca agggaaagtc ttatgatgac tctatgggat
     9721 aatgatgagt ttgtgacaga caatgcactt actgtaaata tgaatagatt gagaagtaaa
     9781 gttaaagaac taggttttga tgattttata aaaactaaga aggggatagg atatataata
     9841 caatgctagt aaaatattta aaaggtaggt ctaagtatat tgctctacct ttagttgtag
     9901 ttagtataat aaatatctac ttatttgcta tagatatttt taaaaataaa tattcagagt
     9961 taatatattt agatttttta gtactgttta taataatggt attttttgtt atagattata
    10021 taaattttag aaatagttac acaaatttat ataattgtat agaaaatagt ggtgaaatag
    10081 acagttattt agttgatgga caatcttttg aagaaaatct aataaaagat attatagaaa
    10141 acttaaaaat aaaaaataat agtgacatag agacttataa acaatcatta aaagaattgg
    10201 atgagtacat agctaaatgg gttcatgaaa taaaaattcc aatatcttct ttaagtataa
    10261 ttacagatag gttaagtagc atagaagata gtttggatat taagaattag gttgcaaaga
    10321 taaatttttt agtaaactct attttgtata gtagtagaag caacactatg tttgaagatg
    10381 tatttataaa taaatttaat ttagaaaaat tggtaaaaat gtctataaaa aataattcat
    10441 ttttgcttat aaaaaataat gtagaagtta gtttgaatga attagaaaat gatgtttaca
    10501 cagattctaa atgcatgtca tatgttttag accaaattat taataatgct ataaagtatt
    10561 caaaggaagt tggtaagatt gaatttaatt caaaaaagtt agaaaataga gtggtattat
    10621 ctataaaaga ttttggtata ggtataaatg aggaagatat atctagggtt tttgacaagg
    10681 aatttacggg gaaaaatgga agaaatcaat tgtataagtc tactggaatg gggatgtatt
    10741 ttgtaaagaa aatgatagat agtttagggc atgagattga ggtatgttcg gaaaatggtt
    10801 catatacaat ttttaatatc tacttttatg atatatcgga ttatttaagc ctagatagtt
    10861 aggttattag actataaaat ctatcatcat atacaaatcg acatatacaa taagaataaa
    10921 tttagttctt gaaaatagtg aagatttaat tagtgcctta aatgatgctc tatctatatt
    10981 ataaaataat aattattata tatgaaaaac atatcagata tgtacctggt atgtttttta
    11041 tttgtagtaa ctattttaat atggtttttg gagagagaat atgttttgtt tgtgaaaaaa
    11101 aattacatgg tattacattg acactgaaaa taaattaatg taagatttag ctaaggtaaa
    11161 aaacgtgtat aagattaact caattaattt cataagagta aattatcgta attaaagatg
    11221 aaataagatt aattctaaaa ttattaaact taataccttg atttggggag gaataactat
    11281 aaacttaaaa ggatgataac tatgtatagt gtaggtttaa tgtcaggaac ttccttagat
    11341 ggagttgatg cagtactagc tgaaattagt gggaatggta gaaatactaa agttaagcaa
    11401 atagaattta taactctaga aattcctaaa gatataaaag acgaaataag aaagtgctgt
    11461 atagaagagg aatcgagtgt tgatttaatc tgtagcttaa attttaagct tggatattta
    11521 tttagtaaag ctgtaaaatc agtgtgtcat aaagcaaact ttcatatagc aaatttggat
    11581 tttatcgcat ctcatggtca aacaatcttt catataccaa gaagttataa taattttgta
    11641 tcttcaacgc ttcaaatagg tgaaccagca gttatagcat atgaaacaaa tacaaaggta
    11701 atatctaatt ttagagtaat ggatatagca gcaggtggag aaggtgctcc attagtacca
    11761 tatagtgaat ttttattata tagtgataac aataaaaatt tagcacttca aaatattggt
    11821 ggaataggaa atattactat aatacctaaa tcatgtaaca tagatgatgt atttgcattt
    11881 gatacaggac caggaaatat gataatagat ggagtatgtc aaagattatt taatagaaag
    11941 tatgataaaa atggatactt tgcatcaaaa ggtaaaatta atgaagaaat gttaaaagat
    12001 ttgatgagtc ataagtatct tagtcaagcg ccacctaaaa gcactggaag agaggtattt
    12061 ggacaagttt atttggataa catgcttaac aaatataaac atgtagataa atatgatttg
    12121 atagcaacag taactatgtt tacggcaaaa gctatatatt ataattataa aaacttcata
    12181 cttccaaagg ttaatgtaga tactttatta attggaggtg gaggagctca taatctaact
    12241 ttaataggat atataaaaga gttattatta gaggttgaag tattaattca agatgaatat
    12301 gggtattctt ctgatgcaaa ggaggctttg gcatttgtaa tcttgggcaa tgaaacatta
    12361 aataattctt tttctaatgt aatatctgca acaggggcta aaaataaagt tattttaggg
    12421 aatataacac ctaaaccttt tggaggtaaa tgacatgtct aataataaaa aaaagaaaag
    12481 aaagaaaaga aagaaaaata ataattataa aaatgcaaaa agtacaaaaa gtaataaaaa
    12541 tactaagaaa caaatggaga aaaatcaaag aacagattat aatattccaa ctataagttt
    12601 taaaagtgga ttaatactaa tagtttcatc tcttgtaagt actgtagttt ttcctttgtt
    12661 attatctata tttggaatag ataatagatt tggagttgtt ataggaaatt cattaattac
    12721 atcttttgca gttgcatata caagatactt tgttgaaact aagagaggtt tttctttggg
    12781 attttttaga ctgtatattt tatttgcttt atcatttgca ataataggtt acttctggat
    12841 atataaagga atacatattt aaaaaagtat tgagactatt ttatgttaat aataagatag
    12901 tcttttttct tttttaagaa aagatatgag tattttaagt agtgtgtaat tttagacaaa
    12961 agaatgatta gataaataaa gcaattaaag caagatataa atgcatgaaa taaaattaca
    13021 atttaaaatg taaaatatga aaaaatatat acaaacgtat tcctgtaatc aaaaatacat
    13081 gctataatga ttgaaaagaa ataaaaaaac agggggaatc aatatgagag tattatttgt
    13141 ttgttcatca gggatgtcaa gtgctatagc agttaatgca ttacaaaaag aaggagcaaa
    13201 aaatggaata gatatagatg ttttggctgt tggtactcaa gagtttgaga atgaagttaa
    13261 aaatggatgg gatattgcta tggtagctcc acaagtcaga catagatttg actatttaaa
    13321 agcttttgct gatgaggtta gtgtaccatg tgcattaata caagcacaag cttatagtcc
    13381 attaggagga ccaaagttat taaaacaagt tcaagaactg ttgagtaaat agtagttgca
    13441 gtgttgttag attagaagaa agttaatttt ataaaaattt taatgataat aaaaataaat
    13501 attaattaag gagagaaaag atgctagata aatttacaga atttttagac actaaattat
    13561 caacttctat ggccagattg gcagaacaac gtcacttaag agctgtaaga gatggaatcg
    13621 tagcttcatt accacttata atagttgggt catttttctt gatattagca tttcctccat
    13681 taccagattc atggggggta actcagtatc taaaggaaaa tgcagttaca atattactac
    13741 catatagaat gacatttttt attatatcag tatattctac atttggcata ggttatagtt
    13801 tggcaaagtc atataagtta gatggattga caggaggttt attagctaca gctacattcc
    13861 ttttgacaat agttcctact gcaatagagg gtcttacccc tgatttatta aagatagttg
    13921 aaaagagtcc agaactttta gcttggtata attcaattcc tcaaggatgg caaatgccaa
    13981 tggctaatct tggtggaggt tcaatgtttg ttgctattgt aacatctata ataggtgttg
    14041 agatttatag atttactgac aaatctaaat tcaagataac catgccagaa gcagttccag
    14101 catcagtcac aaggtctttt gaagctctga caccagccgc attaataata attttaatgg
    14161 ctattgttac ttatttccta gggtttgatt ggcatggctt catagctaaa ttagtaactc
    14221 cattagtttc agccactgac acattaccat cagtaattat actaatattt ttaataacgt
    14281 ttttttggtc atttggaata catggtgtat caataatagg ttcattggca agacctttat
    14341 ggatacaatt attagatgct aatacagctt catatgctgc gggtcaagca gttccaggta
    14401 ttgctgctga accctttttt caatggttta tatggattgg aggttctgga gctacaatag
    14461 gcttagcttt acttcttgca tttagagcaa aatcagctta tggaaagtct ttaggaaaag
    14521 caactgtaat accagcaata ttcaatataa atgaaccaat aatatttgga gcaccaatag
    14581 tattaaaccc aatattaata ataccatttg ttgtagcacc aatagtaatg gcaacagttt
    14641 catggtttgc tatgagtgct aatttagtat ccagagtagt agttactgct ccttggacat
    14701 taccaggacc agtaggggca tatttagcca ctggtggaga ttggagagct tcagttctta
    14761 acttattatg tatagctata tcaataataa tttactatcc atttgtcaag atgtacgata
    14821 agagattgct tcatgaagaa aaagttgaag agcaagaagc ttattaatta gaaactatat
    14881 tggaaattaa aaactatatt agattagtat aaatttcaaa gtataacagt tatgaaataa
    14941 gtaacgaaga tattttgttg attgtttata aaaaaagaaa aggtaaactt ttcttttttt
    15001 ataaatatat atgaaacttt ttttcaccaa atttttaaaa aagtttcgtg taattcgaaa
    15061 taaatttaaa cacatatagg atataaggag atgacacaga tgggaagctt aggaatatca
    15121 atttatccag aaaaaacaac atctaaggaa atatatgatt atatagataa agctgctgaa
    15181 aatggattta cacgtatatt ttcatgttta ttatcagtta aagatactaa agaaaatata
    15241 attaaaaaat ttaaaagtat aaatgaatac gctaagttta aaggttttga ggtaatactt
    15301 gatgtaaatc ccagagtatt tggtgaatta gaaataagct acaaagattt gacttttttt
    15361 aaaaagacga aggcagacgg aataaggctt gatataggat ttacaggttc tgaagaggca
    15421 ttaatgactt ttaatcctca aaatcttaaa atagaaatca atatgagtaa taatactaat
    15481 tatatagata caataatgga ctaccaacca aataaagaaa acttgacagg atgtcataac
    15541 ttttatccac atagatatag tggattaaat ttagaacact ttataaaatg tacagaaaga
    15601 tttaataagt atggattaag aacagcagtt tttgtaacaa gtcaaaatga aactacattt
    15661 ggaccatgga ctgtaactga tgggcttcca acaattgaga tgcacagaaa tctacctata
    15721 gatattcaag taaagcatat aatagctatg aataatataa atgatataat aatatcaaat
    15781 tgttatccaa catatgagga gctaaaggta ttaggttcta tgagaaaaga tatggttaca
    15841 tttgatgtag caatagagga aaatatacga gaagttgaga agaagatact ctttgaagaa
    15901 atacatttta atagagggga catatctgac agtctgataa ggtctactca atctcgtgtt
    15961 aaatataaag gatacaaatt tgaactattt aatactccta aaataataaa aagaggagat
    16021 atagtaattg aaagtagtga atatggtcat tatgcagggg agttacaaat agccttgact
    16081 gatatgaaaa acagtggtaa atcgaatgta gtaggatata taagaaaaga agaattattt
    16141 atattagatt atattaaacc atggcaaaag tttaatttta aaccctcaac aaaataattt
    16201 attatgaaaa tattaattta ttataaaaat ataattatga aagttggaat taaaatgtgt
    16261 ttaaaaattt aatattaaga aaatgaggaa atgatatgtt aataaaaact ttagaaaatt
    16321 tagttacaga aggtagaaat aagaatacat tgcaaataga taaagaagat acattaggca
    16381 taatagaatt aataaataac gaagataaaa ctgtagccta tgctgttgaa gaacaaaagg
    16441 agagtatagc aaaagctgtt aatataatag tagatagaat gaagcaggga ggtagattat
    16501 tctatatagg agcaggcacc agtggtagga taggaatact agatgccaca gaatgtcctc
    16561 caacttacgg agtggatttt gagcttgttc aagctataat agctggtgga aatcaagcaa
    16621 tatttaaagc aatagaaggt gctgaagatg ataaagaact tggaaagcaa gatataatag
    16681 atagaggagt tacatctaag gatgttatat gtggtatagc tgcatcagga aggactcctt
    16741 atgttatagg tgcaatggag tatgctaagg aattggggtg tgcagtacta tcaataacta
    16801 tgaatccgaa tagtgaaatg agtaaaaaag cggatttacc aataaacatt atagtagggg
    16861 cagaagtaat aatgggttca actagaatga aaagtggtac agctcaaaaa atggtatgta
    16921 atatgttaac aacagcttct atggttaaga tgggaaaagt atactcaaac ttgatggtag
    16981 atgttaagac atcaaatgaa aagttggttg aaagagcaaa aagaataata atgatagcta
    17041 caaatgtaaa atatgatgtt gcagaaaaat tcttagaaga agcagataat agcgttaaat
    17101 tggctatatt tatgataaaa agtggtttag ataaggatag tgcaaagagt atcctagata
    17161 gacaagaagg ttatataagt gaagctttaa aatcaataga aaaattgtaa aataaataat
    17221 agtactgata acctttcaat attaaaaaaa cagcattgga aggttatttt tagatattaa
    17281 gtattgaaag aattattaaa gatattgtag gaggatagta tgtatttttt aggtgtagat
    17341 ggtggaggaa cgaaaacaac atttacatta gtagatgaag aattaaatat agtaggaacc
    17401 ataaccaaag gaacttgtca ttataatcaa ataggatttg ataatctaac gaaattatta
    17461 ataactgggt tggaagaagt ttgtaaagat gctaagataa atgtagaaga aataacgtat
    17521 gcatttgttg gacttgctgg atatggaaaa ataaaagaag tattgtatgc attagaagta
    17581 gcaacaaaaa atgcatatag tcatataaat ttactctagg taatgatgta gaaatagcat
    17641 tagcaggttc tttaaatggg gaaaaaggta taaatataat tgcaggtact ggttcaatag
    17701 cacaggcttt agataaagat gggaatcttc acagatgtgg aggatggggc tatgtattag
    17761 gagatgaagg ttcagcgtat tatataggaa tggctacctt aaaaatgttc acaatgcagt
    17821 ctgatggaag gtgttcaaaa acaaaattat atgatttaat aaaaagacat cttaatatag
    17881 agaatgatta tgacataata aaatatgtaa atgatgaaat acaaggagat agaatagaaa
    17941 tagcaaagtt tgctacgata tgtttaaaag cagttattga aggtgataat actgctagta
    18001 aaatatttga tgatgcagct tatgaattaa gtaggcttat aataggatta gagcatcatt
    18061 ttgaacaagg tacaaagata aaggtatctt attcaggtgg agtgtttaag tcaggagatt
    18121 taatattaga gccacttaaa aaatatttga ataaaacgag atttggtata gtgagaccag
    18181 tgttgactcc agatttagga gcatgtttac ttgctaagaa gaaatattat agtaaatagg
    18241 tagaggtgat agagtatgag ttgcatttta aaattaaaac agatatatga agatttaacg
    18301 gaagtagata aaaaaatagc tgattacata cttaataata cagaggctat aagtaagctt
    18361 tctgtatcag aattggcgtc aaactcaaaa acaagtacag caagtatagt taggttttct
    18421 agaaaaatgg gatattcggg ttttggggat ttaaaaattg agatagctaa agatttgatg
    18481 ggcaaagaaa atgaatacac atatgtagat gaaaactatg attgtaatat agatagtgtt
    18541 ataaacaaaa ttacaaacaa aaacatagag actatcaacc aaacaaggtt gttgaatgaa
    18601 aaagatataa taaaagaagc agttgaacag ataataaagg ctaaaaatgt atatatattt
    18661 ggagtaggtg gttcggcttt agttgcacta gatttacaga tgaaactttt aaggataaat
    18721 aaacaggcat ttacctcttt agatagtcat acacaactta tggtatcttc aaatgtagat
    18781 aaagaagata tagctatagc aatatcttac tctggagaat caaaagaagt aataaagtct
    18841 atagaaaatg caaaattaaa aggatgtaag gtcatttgta taactaaata tagtgacaat
    18901 catcttagta agattagtga tttaaagcta gtagtaccaa atatagaaaa aagacttaga
    18961 gaaggagcta tttcttcaag aatagcaatg cttacgttga tagatataat atatatttct
    19021 ataattcaag aaaacttaaa tgaggcagaa cagaaactac aagaaacaaa aaggatactt
    19081 gattatttaa atattaataa tgcttaaaac taggttatat atcaactaaa caaataagtt
    19141 aggctataag cgtaagtcaa taagaaatag ataaatatct aaaatattga cttacttaat
    19201 aataatatat agcattttta taatacaaat gagttgtctt aaaattgatt ttttttaatc
    19261 aaattttaag ttcaactcct tttttatatt tttatagata tatcctatat tttcccagaa
    19321 aacgtattga atatacaaat tataagaagt ataatgttgt tgggagagaa tgtatgaaat
    19381 gtatacaaag ttgattttaa ctgtattatt gataattctc tttactctta ccattattaa
    19441 tgattgaaga caatggtatt tattataggt caaagattcc gaatgtatta atcatcatca
    19501 ttttctcaaa ctaacagcaa ataccctgat ttgcgagttg tattgtatac aaattatcta
    19561 gaaaatatct attagatttt ccataaaatt taaatatttc acgaatgtat gaatggagaa
    19621 atatacatgt tgacaatcca cctttttaaa tcctaaatta attttattaa tggtagagaa
    19681 taggaagtaa caatgtttta taaaggagaa tattaagtat gaagaaaatt aggaagttaa
    19741 ctacaaagtt attgattaca acaatagttt taatctcagg tatgagtatg acagtttatg
    19801 gtatgacagc caaagaggta acagcaaaaa caccaaaaag ctatgtcact ggaaccaata
    19861 gcgtatatgg tccaaagctg tcacaagccc aactaaactc agtagcacaa gcaacagcag
    19921 attttatgaa caagaaaatc acaaagaata tgaccacaga tgctaagatt ttagttgcct
    19981 acaatcacat taaaaataac acaacatatg ttgactggaa tgcagtagag ggagcaaata
    20041 ctgcctatac attggttact aaaaaaggtg catgctcagg aatggctagg agcatgaaag
    20101 ctttgtgtga tgctatggga attgaatctt attatgtaca cagcacatca aatgaccacc
    20161 agtggaatct tatccgtttt ggtgatgggc gtttgtatca tgtagatatt gatgctaata
    20221 aatcagcagg aaaggatatc atatacaaga gtcttagcca cccattacca tttgataaga
    20281 cagcatatcc aaaagttggt gcaatagtac agacggtaaa accagaacag acacagaaac
    20341 cagcaccacc tggaaagcac actatgttac cacatcaaga aggggcaaag gctgttggaa
    20401 attttggaaa aaaaggagaa gatataaatg aaaaacatgt aatgtatgaa ggtgttagat
    20461 gtagtgtagt taattcatca gtaaatacaa ttacatttac aggtagtgaa tcaaatttag
    20521 acccatcaaa tcttacgatt gctgtaatag gatttatgaa cacaccatat gggatgtttg
    20581 cattagcacc agagggtaaa cctgttgaat atccttttaa atatgataag ccattttctt
    20641 tagattttag tggtaaaaat cgttctggtg cagaattagc gaagtactgt aaaaaaaatg
    20701 atgtgactat taaaatagaa atcactgatg aaaaagctaa tattggactt ggtcaaataa
    20761 tgtatgttcg atatgaataa tgatttaatc atgacagtag aataagttat aaacattgcg
    20821 ccctcttact aggggcgcat ttaatattat atctgatatt ttgtagtcag gtcaactgct
    20881 agatttctcc cctttttatt tataaaaatt attatctcta agcatctgag catgtttacg
    20941 attaactaaa aactgtttta actcagtcaa gcataggaat aaaatagctc tattttcaaa
    21001 ctctgttctg tcaacaggta gaggctggtt tttcatacta agcattagtc tctttaattc
    21061 ttcaagaaga agattgcctg tttcagcttc aaattcagta taacctatct tatgtataaa
    21121 agcagataga atatgagctt gtggtggtgt agaatttagt tgagatacat aactatacaa
    21181 attttctaag atatcccgtt gactttttat aatatccata tgctctaaga agaatctagt
    21241 atcacttaat aaagtattat taatattatc gtaagtttca gaaatactgc tgtcaataag
    21301 tgaatttaat gtatataaat ctctactata tccattttct tttttaggat ttacaataat
    21361 atctgcaatg tctataagta caatactcac ttcttcctgt aacttttttt gaacttcatg
    21421 gattttatgt atatttgttg ggatatacat atttattaag acacctattc cagttcctac
    21481 tgcaaatatt ccagcttcat ttaaaatcca tttgatagaa gtttctcctt gaaggtagta
    21541 atgggatgct atgactacac acatagcaat aacatcttga atatttagca aaaaacaaag
    21601 tggtataatt ataaatataa atattgaaaa tgaagtcaaa ttatatccta aaatattaaa
    21661 acataaatat gaaaaaatag tacataaaag aaaaccataa atctttccaa tggagccttt
    21721 aagagtttct cttttagtgt cctttactgt aagtagtact ataactccag caaccattga
    21781 gtattctaat tttaatagcc aagcaataaa tatagcaaga gaacatccaa gagataattt
    21841 tattacttta agtgaattaa tattattcaa atgcaaacct cctgtaaatc aatataataa
    21901 aattctaaca taaaactaat attgcttgaa aggtaagtaa ctatacttaa ttattttatt
    21961 gccctatttg tttagataca atcaatataa ttttatttta tgaaaatttt ttgtaaaatt
    22021 ttaaatttag agactaacat gagaccacaa agaggaagtt tattataaga agaaaactta
    22081 aaattgattt taaatagcaa atgaaaataa tcaaatatgt atatactatt attaaaaaat
    22141 aaaattatat agttaatata attttataat tggtttagta tagagatttt taacttttat
    22201 gttatactat ttataaaagt aggtataaag attaaaatac ataatcaaat atgtataaga
    22261 ggttttaaat agtattgtta atgaacttga ttattgttta taattacaaa ttggaggaga
    22321 taaacatgaa aattactgaa ggatatatgc cttttaaagg ttttaaaacg tattaccgta
    22381 tagtaggaga aaatacggaa gggaagaagc cattagtatt acttcatgga ggtccaggtt
    22441 ctacacacaa ctattttgag gtattggata agatagctga aagtggtaga caagtaataa
    22501 tgtatgacca aattggttgt ggaaattcat ttgtagaagg gcatcctgag ctatttaatg
    22561 cagatacttg gatagaggag cttattgaac taagaaaaca tctaggactt gatgaaattc
    22621 atctgttagg tcaatcttgg ggaggtatgc aggctatttg gtatgctatt gaatataaac
    22681 caaaaggtat taagtcttac atcctttcat ctactctttc ttctgcaaaa ctttgggaga
    22741 aagaacaaaa aagaagaata tcatatatga gtgaggttga ccaaaaagca ttacttgatg
    22801 cagtaaatac tggagattac tctagcaaag aatataatga tgcattagaa agatttatgg
    22861 aaatgtattg tgcaggtgaa gtgacagaag attctccaga gtgcctgaga agacctaaaa
    22921 aatcaggttc tgaagcatat attgtaggat ggggacaaaa tgaattttct cctacaggaa
    22981 ctctttctgg atatgaattt acagatagat tacatgaaat aaaagagcct tgtctagtaa
    23041 ctagtggagc aatagattta tgttcaccat atattgcaaa gactatgtat gatagaatac
    23101 ctaatagtaa gtgggaatta tttgagtatt caagacatat gccattcgtt gaagaaaatg
    23161 agaaatatat aaaagtctta actgagtggt tgaatgcgaa cgattaaaaa ctatataaat
    23221 ttatataaaa aaatgagtgt ctcaaaatga actttttagt tcattaggca ctctttttgc
    23281 atgaaatcaa atatattttc attatttgaa cgataaaatg aagcttatat ctattttggg
    23341 aaagtaattt tttattacta gcgttagatt ctaatctaat tgtatactta ttgaaataat
    23401 agaaatatat tggtgtttat aacttaattt tattgttttt tagaaatttt tttatatatt
    23461 ggtataatta ttctgtttaa atttgattaa aaagtatatt aattataata agaaacaatt
    23521 atataaatag gaatagatta ttataattaa tatagatata aaaataatta tagaaggaaa
    23581 ggcacattat ggaaaaattg agagttattg aaatgataaa aaataatccc aatattatag
    23641 ctacaataga taatccaact gatgaaatga aattgttagc tattaaagaa aatgggcttg
    23701 tactagaata tataaataat ccaaccagag agatgcaaga actagcaata gataataata
    23761 ttagagcaat taaatttata gataatccaa cagaagatat gatgataaaa gcagtaaatg
    23821 agggatggag tatactggat tatatcaaaa acccaactga taaagttatt gaaatggcta
    23881 taaatcaagc aggatgggca attaaatatg ctaaaaatcc aagtgaagaa ttacaactat
    23941 tagcagtaag aaaaaattat gattcgataa agtttataaa acaaccttat gaaagtgtac
    24001 aagaggaggc agtgagaata agttatgatg ccttgagata tatagattca cctagttaca
    24061 atgcagaact tatagctata aaaaataatg aagcagctat tagctttata actgatttag
    24121 ataaaaataa aatgttggaa tttttaaaag tgaatatttt agtgattaag tacattctga
    24181 aaaaagtatc taaagatgaa ttagagagtg tattaaaaga agtattatcc aaagaagatg
    24241 ttgaggaaaa gtatattaga gattttttga attgtagtgt tatagataga aatagcgaaa
    24301 gtataccaat agataaaatt atgtttattt ataaatatgg aagtaagaca gcaaaaaaaa
    24361 tagctgtaga tgagaaatta aagattatgt aggagagaga aaaatgaatt ttatagacac
    24421 attaaaaagc gttgacttaa tattatccac tggagaagta cctttaatag taggtgaaag
    24481 tggaatagga aaaacagctt tagcaaataa gcttgccaaa gaaaatgatt ggagtttaat
    24541 tgttattgat ggaaacttac ttaaagaggg tgaaataggt ggtcttccaa ctatagaatc
    24601 ttatgtagga gttaattcta atggatataa gactgaaaag aaaacaacag tgtatgctgt
    24661 tcataacaaa ttaagagaaa ttgatgaaga gatatctaaa agtaagacag ttcttttatt
    24721 tatagatgag ataaatcgtt gtgagcatac agtacagcaa gaacttatga atttaatttt
    24781 aaatagagaa attaatggat ataaactgca tgatgatgta aagattttag cagcaatgaa
    24841 tccatcaagt aaatatggtt cagattttga ttatcaagtg gtagatatgg atgcagccca
    24901 agaaaatagg tttgtttggt taaatatgga gagtgaccat acacaatgga taaaatgggc
    24961 aatagatgag ggtattgaaa gaaaagttat cgagtttatt tccacttttc cagaatatct
    25021 gcataaaata aatgaagatg atgtaagagc cactcctaga agttatgaga gagtatctaa
    25081 aatttataaa gtttataaag aaaaaaataa ttctattcct agagcagtat ttttaaatgt
    25141 tgtaaagggt aatgtaggta aagttattgc agaagaattt ataagtttta ttgaatcaaa
    25201 ctctgaacca ttaatatcct atgaagatgt ttttttagga gaatctattg atgagtctat
    25261 tgtagaaaga gtaaaaaatg agagtcatac aagactttat ctatcagcta tgaacattct
    25321 aaaagactta gaattaaata ttaagaatga taaatatgaa tcaaaccact atattaatag
    25381 atttattgaa tttttaaaaa tgtatcctgt agatttaatg ataggtatta tgaaggatat
    25441 tagaaacagt tatattgaag tatataaaaa agctatagaa aatgaagagt ttgtaaaatc
    25501 atattttgaa tcttatagtt taataagggg ataatgtatg gaaaattatt ttgataaaca
    25561 agcaaaagag ctttataata aagcaagtga aattataaat actcattcta tgctaaaggc
    25621 aaatcgtgct aatgagaaat ttgatataga tataccacaa gattttaagt atgagttttt
    25681 tagtcttgta gataaagtga atttaagtct tatggaagaa gaagataatt tttatggata
    25741 ttttttgttt cagatgtcaa gagaaataag atttgatata agtagtccta caggtgtgaa
    25801 ttttaaggga gctaaatatg ttatatattt taatccaata atctttttaa cgcttgacat
    25861 aaagcaaatg gagactacaa ttaaacatga aattcatcat atattatcta tgcatttgat
    25921 gagagcaaag gagttaaaag gaaagtatag tacattagca attaatatgg caatggatat
    25981 agtagtaaat aaatttctaa acaatttacc accatatgca actaccttag agtgggtaaa
    26041 tttaaagtat tccttaaaac ttgaaccata tgaacctttt gaatattatg tagaaaagat
    26101 tcagactgaa ttagatttga tggaagtgga tgaagatggt gaggaagatg atagtgataa
    26161 atatggagat atagaaattg attatagccc cgaaagaact catgatatat gggatgaatc
    26221 caatgagata gatgaaaata cccttagaga atttactgaa aaatttatta acagttctca
    26281 aaaaggtaaa gttcctgctt atttagaaag tatgatatca tcacttaaaa atagtaaggg
    26341 tgaattgcca tggaatttat atcttaatag aataatggga acagttgaaa ataataaaaa
    26401 gaagactata acaagaagaa atagaagaca accaaatcgt ttagatttga ggggagaact
    26461 tagaagtcat aagtcagaaa tagcagttgc acttgatgta agtggaagta ttagtgatga
    26521 agagtttaaa caagctatta aagaagttct tagtatagtg aaaaactata atcatgaaat
    26581 tacaattata gaatgtgata atgaaattag acgtgtatat aaagtgagaa ctgaaaaaga
    26641 tataaaagat agaattagga taggtggagg cactagattt tctccagtct ttgagtatgc
    26701 taataataaa aagattaatt tgttagtcta ctttactgat ggtaaaggtg aaaataaact
    26761 acaagtaata cctagagggt ataaaatttt atgggttatt tctggaagag gagataaaat
    26821 ttcattaaaa gaaccttatg gagcagttaa aaaacttact aaggttaaaa caaatgatga
    26881 aatattagaa ataaatgatg taagaaatga tgggtactca atgaatagcc aagcaccaat
    26941 tttataggta ttcacaggag ataagcttta ttaatttatt ttgatatgct tgttataaaa
    27001 atctaaaaaa tacattaatc aatggttaat gtatttttta attttaataa tataagtttt
    27061 acttacattt aagtcttaaa atgtgttaaa atataatata tagcagatag ttttggcaag
    27121 gtggatttag ttgtcaaaac tatttttttt attaaggatg gtgatgtttt tgtttaagat
    27181 tgatgattat ataatgtatg gtatgacagg agtctgcaaa gtcatggata taacaaatga
    27241 acgatttaca aatggaataa aaaaagagta ttatgtattg agtcctattt attctaataa
    27301 tacagtaata aaaattcctg tagataatga aaaagtacca atgagaaagt tactttctaa
    27361 aattaatgtg ctttcattaa taaatgatat tcctaatatg gatacatcat ggatagataa
    27421 tgaaaaattg agaagtgagc aatttaaaaa aatactcaga ggtggtaaat gtgaagagtt
    27481 aataaaatta gtaagaagta tagataataa cagagagtat gtaaaatcta ttggtaaaaa
    27541 aacacatcaa gcagatgata atataatgaa agaagctgaa aggttattaa atgaagaatt
    27601 tgcaactatt ttagatattt ctcctaatga agttagttca tatatttcaa gtcatatacc
    27661 tcaataaagt gttttagtat cctttattta atctatttta agtattattt atgtatgctg
    27721 gttttggtaa aataaaatat ataattaaat tatataaaaa ataataatga ttgtattgaa
    27781 gaattacaga atagtgcgga gagcctactc ctttaggtgt gagatggata tcacttaaag
    27841 atttaaattg ttattgtaga tgtttttaat ataaaacaag attaataagt gtattgtaag
    27901 ctttaagtga aattgatact ataatgagtt acttagattt taaaatatat aatattataa
    27961 aaaatccttt taggttttga atgaataaga tggatactat gttgaataga atccatctta
    28021 tttaagtttt atttatgttg tttatttgta agcattattt ttgtacatga aaacacagtg
    28081 taaaaatatg cttagaatta tgaagtttta ttaaaaagat attcgacaaa ataagactaa
    28141 aaatattctt aaacttataa ctaagataaa tattgtatat ttatagtgta gcctgaaaaa
    28201 ttgtgaa
//
LOCUS       DAEMIK010000037        28199 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEMIK010000037 DAEMIK010000000
VERSION     DAEMIK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.037"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..196
                     /locus_tag="KPK87_001375"
     CDS             35..196
                     /locus_tag="KPK87_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537538.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALIAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            280..810
                     /locus_tag="KPK87_001376"
     CDS             280..810
                     /locus_tag="KPK87_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2537539.1"
                     /translation="MCKILLVRPSKQYADQVMSYKEEMSQNGDSFDGCAGLENVQSFE
                     EWIDFEARLKAKYKDGYVPSEVFLAIRKKDNYVIGMIDFRHPLSDFLFNFGGNIGYSV
                     RPSERQKGYASEMLRLILPICHEFGESKVLLTCDKKNEASQKTIVRNGGVLEKEIPDT
                     VGLSESGIIQRYWISI"
     gene            885..1427
                     /locus_tag="KPK87_001377"
     CDS             885..1427
                     /locus_tag="KPK87_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2537540.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNHCGGCGNGNQSCKIARC
                     SLEHGMIEYCYECKQYPCEKYQHIDDYDIFITSRRQKADLERAKIIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EMFQKIADRRNIKLKLIKKK"
     gene            1531..1827
                     /locus_tag="KPK87_001378"
     CDS             1531..1827
                     /locus_tag="KPK87_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537541.1"
                     /translation="MNDIFKDMQAKVGCENISDLPSYKRKVWHEMKQLNFADYEEKQL
                     EDFSNYVFGMSCQTLKDVMKQQKGREEQCRKQGCWWKRAEQLAKKQHHTGSTCR"
     gene            1746..2249
                     /locus_tag="KPK87_001379"
     CDS             1746..2249
                     /locus_tag="KPK87_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2537542.1"
                     /translation="MQETRVLVETSGTTGEETTSYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPFADEVHEHTSVYQLNELDFMYRQLSSDMQEEYTALLTVYENLEALYICR
                     NVIAVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLLENRRYLVTEH
                     AVFELPE"
     gene            2267..2770
                     /locus_tag="KPK87_001380"
     CDS             2267..2770
                     /locus_tag="KPK87_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2537543.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQC
                     EEYAIHDTDNFPIEIGEYVSIEELNEMYEMIEELPDYIVECLDEFIGHYGTLEEVMEH
                     KDNIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            2880..3269
                     /locus_tag="KPK87_001381"
     CDS             2880..3269
                     /locus_tag="KPK87_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537544.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFFI
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLDKQILNETITAVRSVNYVPD"
     gene            3256..5706
                     /locus_tag="KPK87_001382"
     CDS             3256..5706
                     /locus_tag="KPK87_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2537545.1"
                     /translation="MYPIKYIDNNLVWNKDNEVYAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLREVAVRKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEELLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIDDFGYLMEHLYGRDGIAYEDYEYQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKSVRSVTQSETRGLLHVIDELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFNEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            5706..7841
                     /locus_tag="KPK87_001383"
     CDS             5706..7841
                     /locus_tag="KPK87_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2537546.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTMAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNENNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNASDSQSMGRRIFSRP
                     YLYMAHRARRMERRLASAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTYSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDMPTQTAYAVYSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATSGATRPHERPATAPT
                     SKTSKPVKINSAKKRPLSSGVSDVKAPQSAQPVHRQNVEKVPVSEKTRQNFKAERTTK
                     AHIVQQPKKITETTEKNRNAIFTKGQKKK"
     gene            7838..8845
                     /locus_tag="KPK87_001384"
     CDS             7838..8845
                     /locus_tag="KPK87_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2537547.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTADLQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLNSSYWKQHLIGAGRVK
                     Q"
     gene            8861..9763
                     /locus_tag="KPK87_001385"
     CDS             8861..9763
                     /locus_tag="KPK87_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537548.1"
                     /translation="MFKKNKKTTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISD
                     YLTKELQDLNVDTIRTDIPTSSAVTDVIVWSIEQLGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDADGDMVIIQNPTLAPAIEKSEYEPKTLETDASVDADTVNDATAFLE
                     TFFNLYPTATEKELAYYVARNVLEPIGKDYLYSELVNPIFIKDGDNVKVKVAVKFLDN
                     QTKATLVSQYELVLHKDSNWKIVG"
     gene            9866..10681
                     /locus_tag="KPK87_001386"
     CDS             9866..10681
                     /locus_tag="KPK87_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG2537549.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALDCSNDTIIEQLDKKSIEIMDNIQKEQEKLKKIE
                     IAKKELLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            10762..11238
                     /gene="tsaA"
                     /locus_tag="KPK87_001387"
     CDS             10762..11238
                     /gene="tsaA"
                     /locus_tag="KPK87_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBG2537550.1"
                     /translation="MTTYKVNSIGTIYNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTDFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSVVEIINIDYMNGMI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPNVPDWCSHWPHSLEESASFAWENEFNF"
     gene            complement(11398..12582)
                     /locus_tag="KPK87_001388"
     CDS             complement(11398..12582)
                     /locus_tag="KPK87_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBG2537551.1"
                     /translation="MILVGIDIGKKQHTFSIIDKGTGEILSNPSVFNNNQKGFLLLIG
                     KLSSYVKSELLIGMEDTGHYHFALLKYLLERHYTVALINPTTTDLTRKLQGGITKNDP
                     LDSLTICDVIGSNQRKKPYRITKVNRFDLYEQKQLTRHHHNLKEELNIYKNRLQKCID
                     IVFPEFNSLFRSKYGIVYMNVLKTFASAEKIANSDIRIIRKCFEYEGRGKRIPLSAEQ
                     LKTVAKASVGIPSVAEEIQIRHLVCQIEMIEEQLSEIDKKIEEFSLQNNSPILSIPGI
                     SHFSGTSILAELGDICNYTKASQIIKFAGVAPYHYESSQFTAQHTAITKKGSRYLRKT
                     LYQIILPVINHNRVFTAYYNKKLAEGKGHRCAQGHCIRKLLRIIYHLLSTGQTFDPAL
                     LI"
     gene            12889..13440
                     /locus_tag="KPK87_001389"
     CDS             12889..13440
                     /locus_tag="KPK87_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2537552.1"
                     /translation="MSLQDYIIEKPVIDTEHLILRTMDKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPEVLFSKPEKATKSFHWRIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKQYQGNGLMSEALKSIIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITS"
     gene            13707..14618
                     /locus_tag="KPK87_001390"
     CDS             13707..14618
                     /locus_tag="KPK87_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2537553.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKK
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHIIPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     AEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEVWIIPV"
     gene            14696..15130
                     /locus_tag="KPK87_001391"
     CDS             14696..15130
                     /locus_tag="KPK87_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2537554.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            15595..16338
                     /locus_tag="KPK87_001392"
                     /pseudo
     CDS             15595..16338
                     /locus_tag="KPK87_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            16289..16900
                     /locus_tag="KPK87_001393"
                     /pseudo
     CDS             16289..16900
                     /locus_tag="KPK87_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            17113..17970
                     /locus_tag="KPK87_001394"
     CDS             17113..17970
                     /locus_tag="KPK87_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2537555.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYIMEDMLHNVYDATTYGIETFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EVTLYKYLG"
     gene            18048..18629
                     /locus_tag="KPK87_001395"
     CDS             18048..18629
                     /locus_tag="KPK87_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2537556.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            18733..19578
                     /locus_tag="KPK87_001396"
     CDS             18733..19578
                     /locus_tag="KPK87_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537557.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISKNRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            19575..20258
                     /locus_tag="KPK87_001397"
     CDS             19575..20258
                     /locus_tag="KPK87_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537558.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            20255..20953
                     /locus_tag="KPK87_001398"
     CDS             20255..20953
                     /locus_tag="KPK87_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537559.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKFGIPFLER
                     ITDFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPVPFLIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            20965..21876
                     /locus_tag="KPK87_001399"
     CDS             20965..21876
                     /locus_tag="KPK87_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2537560.1"
                     /translation="MEKGRLLQNKNINKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSEQYNTLSAPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            21928..22068
                     /locus_tag="KPK87_001400"
     CDS             21928..22068
                     /locus_tag="KPK87_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537561.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            22359..22985
                     /locus_tag="KPK87_001401"
     CDS             22359..22985
                     /locus_tag="KPK87_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2537562.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNVLYQKCLVNGPISYIEDIFNEMIKKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            23107..23823
                     /locus_tag="KPK87_001402"
     CDS             23107..23823
                     /locus_tag="KPK87_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2537563.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            23987..24178
                     /locus_tag="KPK87_001403"
     CDS             23987..24178
                     /locus_tag="KPK87_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG2537564.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            24202..24432
                     /locus_tag="KPK87_001404"
     CDS             24202..24432
                     /locus_tag="KPK87_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537565.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            24699..25118
                     /locus_tag="KPK87_001405"
     CDS             24699..25118
                     /locus_tag="KPK87_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2537566.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVEGLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            25121..25375
                     /locus_tag="KPK87_001406"
     CDS             25121..25375
                     /locus_tag="KPK87_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2537567.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            25750..25953
                     /locus_tag="KPK87_001407"
     CDS             25750..25953
                     /locus_tag="KPK87_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2537568.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            26031..27221
                     /locus_tag="KPK87_001408"
     CDS             26031..27221
                     /locus_tag="KPK87_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2537569.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDMEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     KLAS"
     gene            complement(27415..27732)
                     /locus_tag="KPK87_001409"
     CDS             complement(27415..27732)
                     /locus_tag="KPK87_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537570.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(27754..28092)
                     /locus_tag="KPK87_001410"
     CDS             complement(27754..28092)
                     /locus_tag="KPK87_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537571.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         9388 a         4620 c         5877 g         8314 t
ORIGIN      
        1 atggttttta agtaacgccc agagcttagt gcttatggcg attgtggtta ttggtatcta
       61 cttagggttc aaacgtgagt tttcaaaact tatcggcttc ttggtagttg ccttgattgc
      121 tgtcggtttg gtattcaatg ctggcggtgt gaaagacgta ttgttagagc tgttcaataa
      181 gattatcggt gcgtaaaatt ttattgtggt gctgatatga gaatgctata atgtggatat
      241 gaagaaagcg acaaatggga attttggggg agaagtaaga tgtgtaaaat attgttagtc
      301 agaccgtcta aacaatatgc agaccaagtt atgtcatata aagaagaaat gtcacaaaat
      361 ggagatagtt ttgacggttg tgctggtctt gaaaatgtac aatcatttga ggaatggatt
      421 gattttgaag caagattaaa agcaaaatat aaggacggat atgttccatc agaagtgttt
      481 ttggctatac gaaaaaaaga taactatgtt attggcatga ttgattttcg acacccatta
      541 tccgacttcc tctttaattt tggtggtaat atcggatata gtgttcgtcc atcggaacgg
      601 cagaaaggtt atgcgtccga aatgttaaga ttgattttac ctatctgtca tgagtttggg
      661 gaaagcaaag ttttactaac atgtgataaa aagaatgaag catcacaaaa aacgattgta
      721 agaaatggtg gcgtgttgga aaaagaaatc cctgatacag taggacttag tgaaagtgga
      781 attatccaac gatactggat ttcaatatag actctctttc atcaaatcca gtttgtaaaa
      841 gtcaataaat acaaagataa atttgaagtt aggagactaa tactatgaaa ggatttgaac
      901 gcaaaaacca attattctct ctttgtggat tgaattgtgg gttgtgtcct atgcttttag
      961 gaaatcattg tggcggttgt ggcaatggta atcaatcatg taaaattgca agatgtagtc
     1021 ttgaacatgg aatgattgaa tattgctatg agtgcaaaca atatccgtgt gaaaaatatc
     1081 aacacattga tgattatgat atttttatta cgagcagacg acaaaaagca gatttagaaa
     1141 gagcgaaaat cataggcatt gagcaatata atcttgaaca gcaggaaaaa acacaaattc
     1201 tttcttactt actttctaac tataatgacg ggcgtagaaa gactttcttt tgtgtagcag
     1261 tcaatttatt agaactttca gaattgcaag aagcaatgaa acaaatacaa tcaaatgacg
     1321 aattgacctt actaccattt aaggcacaat gcttgtatgt ggtagagatg tttcagaaga
     1381 ttgccgacag aagaaatata aaactgaaac ttatcaagaa aaagtgattt ttaaatttca
     1441 gtttgacaac ttcatatcca tacacacaaa gcagttagtc ttaagatcga ctgctttttt
     1501 cttactttaa tttcagattg gagtgatgaa atgaacgata tttttaagga tatgcaggca
     1561 aaggttggct gtgaaaacat ttcagacctg ccctcttaca aacgtaaagt gtggcatgaa
     1621 atgaagcaac tgaactttgc tgactatgaa gaaaaacagt tagaagattt ttctaactat
     1681 gtgtttggta tgtcatgcca gaccttaaaa gatgtgatga aacaacagaa aggacgtgag
     1741 gaacaatgca ggaaacaagg gtgctggtgg aaacgagcgg aacaactggc gaagaaacaa
     1801 catcatactg gttcgacctg ccgatagatg ttgccgagtt tgaagaaaag ttaggtgtcg
     1861 gtgcagaaag tggggattac cgcattattg aaaaggtact gccctttgct gatgaagtcc
     1921 acgaacatac aagcgtgtac cagctcaacg aattagattt tatgtaccgc cagctttcaa
     1981 gcgatatgca ggaagaatat acagcacttc ttaccgtata tgagaactta gaagcacttt
     2041 atatttgcag gaacgtgatt gcggtttatc ccgactgcaa aagcatgata gatgttgcaa
     2101 gacaaaagct gatgaacgac ccgacgttca aacatttatc cgaggactgt caagaatact
     2161 actttgactt tgaagcctat gcttctcact tactggaaaa cagacgctat cttgtaacgg
     2221 aacacgctgt ctttgaactg ccagagtaga aaatgaggtg gtacttatga ttgatgatat
     2281 ggcagtttat atcgctaatc ttggcaagta caatgagggc tatttagtcg gtgcttggtt
     2341 cactttccct attgatgagg aagatgtgaa agaaaaaatt ggtttgaatg aacaatgcga
     2401 agaatacgca atccatgata ccgacaactt ccctattgag attggggagt acgtttccat
     2461 tgaagaactc aatgaaatgt atgaaatgat agaggaactt cccgactaca tcgtagagtg
     2521 tttggacgaa tttatcggtc attacgggac gctggaagaa gtcatggaac acaaggacaa
     2581 tatttattat tatcccgact gtgaaaccat gacagacgtt gcctactact acatagacga
     2641 attgcaggca cttggagaca ttccacccag cttgcagaac tatatcgact atgaagccta
     2701 tgggcgagat ttagatatgg gcggttgctt cattgcaaca agccgaggta tgtgcgagat
     2761 accatattaa cgctggaaga tcagcgacaa gtattgttgc tactgacagc gtatttctct
     2821 tttaagggac agtctgaaac atggctgtcc ttttccattt tgaaaggagt tgaaagaact
     2881 tgaaaaagat taaaagctac acgggtatct ggaatgtaga aaaagtctta tatgcaatca
     2941 atgactttaa cctacctttt ccagttacct ttacacagat tacatggttt gtgattacag
     3001 agtttttcat cattctgttt ggggatatgc caccactttc catgattgag ggagcatttt
     3061 taaaatactt cggtattccc gttgctctta cttggtttat gtcccagaaa acgtttgacg
     3121 gaaagaagcc gtacagtttt ttgaaatcac agataaccta tgccctgcga cccaaaatca
     3181 cttatgcagg aaaagccgta aaactggata agcagatatt aaacgaaacg attacggcag
     3241 taaggagtgt gaactatgta cccgattaaa tatatcgaca acaatcttgt ctggaacaag
     3301 gacaatgaag tgtacgccta ctatgagctg ataccttaca attacagctt tctatctgcg
     3361 gaacagaaat ttattgtgca tgacagcttt cgccagctaa ttgcacagtc ccgtgagggt
     3421 aaaatccatg ccttgcagat tgctacagaa agctcaatta gaagtatgca ggaacagtcc
     3481 aaacgattgg ttacggggaa acttcgtgag gtggcggtaa gaaaaattga tgaacagacc
     3541 gaagcgttag tatctatgat tggggacaat caagtggact accgcttttt tcttggcttc
     3601 aagctcatgg taacggaaga actgctcaat ctgaagaaca tcaaaaaatc ggcgtggctg
     3661 acattcaaag agtttctcca tgaagtgaac cacacgctga tgaatgactt tgtttccatg
     3721 ccgaatgatg aaatcaaccg ctacatgaaa atggagaagc tgttggaaaa taaaatctca
     3781 aggcgtttca aagtacgccg attagaaatc gatgattttg gctatctcat ggaacatctc
     3841 tacggcaggg acggtattgc ctatgaagat tatgagtatc agctaccaaa gaagaaatac
     3901 aaaaaagaaa cgctgataaa atactatgac cttatccgtc cgaccagatg tgtgattgag
     3961 gaaagccagc gatacttacg actggaacat gaggacaagg aaagctatgt gtcctacttt
     4021 accgtcaatg cgattgtggg ggaacttgat tttccgtcct ctgaaatctt ttatttccag
     4081 caacagcaat tcacattccc cgttgatacg agcatgaatg tagaaatcgt ggaaaataga
     4141 aaagctctta ccacagtacg caacaagaaa aaggaattga aagaccttga caatcacgcc
     4201 tatcaagcag gaagtgaaac aagctcaaat gtagtggacg cattagacag cgtggacgag
     4261 ctggaaactg atttagacca gaccaaagaa agtatgtata agctaagcta tgttatacgg
     4321 gtgtctgcac ccgatcttga cgagctgaaa cgccgttgtg atgaagtcaa ggacttttac
     4381 gacgacctca atgtaaagct ggtgcgtcct gctggggata tgctggggct tcattctgaa
     4441 tttttacctg ccagcaagcg atatatcaat gactatgtgc agtatgtaaa atcagatttt
     4501 ctagctgggc ttggttttgg tgcgacacag cagttagggg aaactacggg tatctatatg
     4561 ggctattctg ttgatacggg aagaaatgtg tacctgcaac cgtcccttgc cagtcaaggc
     4621 gtaaaaggaa cggttacaaa tgcccttgct tctgcatttg tgggttcgct tggcggtgga
     4681 aagtcgttct gtaacaacct gcttgtgtat tactctgttc tctttggggg acaagcggtt
     4741 atcctagacc cgaaatcaga gcgtggcaac tggaaagaaa cacttccaga gattgcacat
     4801 gaaatcaata tcgtaaacct taccagcgat aaggacaacg caggacttct tgacccgttc
     4861 gtgattatga agaatgtaaa agacgctgaa agtcttgcga ttgatatact gacattcctt
     4921 acgggtattt cttctaggga cggcgaaaaa ttccccgtac ttcgtaagtc ggtgcgttct
     4981 gttacccaga gtgaaacaag gggcttgctt catgtgatag acgaacttag aaaggaagat
     5041 accgcaatct cacgaaatat cgcagaccat atcgacagtt ttacagacta tgactttgca
     5101 catttattat tttcggacgg aacggtggaa aatgctatca gccttgacaa ccagctcaat
     5161 atcattcaag tagcagattt ggtattgcca gataaagata ctacttttaa cgaatacacg
     5221 accattgaat tattatcggt ggcaatgttg attgttatta gtacctttgc ccttgatttt
     5281 atccattcgg acagaagcat ttttaagatt gttgatttgg acgaagcgtg ggcgttcttg
     5341 aacgtggcac aaggcgaaac cttatctaac aagctggtac gtgctgggcg agctatgcag
     5401 gcaggcgttt attttgttac ccagtctgcc tatgatgttt caaaggaaag tttgaaaaat
     5461 aatatcggct taaaattcgc tttccgttct actgacatca acgagataaa gcagacctta
     5521 gaatttttcg gtatcgacaa ggacgacgaa aacaaccaga aacgcttacg agatttggag
     5581 aacggacaat gtttattgca ggacttatac gggcgtgtcg gtgtggtgca gatacaccca
     5641 gtctttgaag aactcttaca tgcctttgat accagaccgc ccgtacagag aaatgaggtg
     5701 gagtgatgaa agaaaggata cagagtgcgt tcacaaaaaa gaagattttc cactttctca
     5761 aaatggctct atttgtcgtg gcactttccc ttatcctgct ttcacttctt gggacgatgg
     5821 ctcatgcaac gggacttgtg gacgatacca taaatgcgga aaatctttac tcaaaatatc
     5881 ctctttccaa ctaccagctt gatttttatg tggataatag ctggtcgtgg ttgccgtgga
     5941 actggctgga cggtatcgga aaatcggtac agtacgggct ttactgtatc actaattttg
     6001 tctggacgat aagcctttac ttatctaatg ccacgggcta tgtggtgcag gaagcctata
     6061 aacttgattt tattaacgat atggcagaca gtatcggaaa aagcatacag acacttgcag
     6121 gcgtaacaca gaacggtttt tcttctacgg gcttctatgt gggcttcctg ctactcatta
     6181 tcttagtggt gggactatat gttgcttaca cggggctgat aaaaagagaa accagcaaag
     6241 cacttcacgc tgttatcaac tttgttgtgg tgtttgtatt gtcggcttca tttattgcct
     6301 atgctcccga ttacatcaaa aagataaatg aattttcatc agacatcagc accgcttctt
     6361 tggacttggg aacaaaaatc atgctcccca attccgacag cgagggcaag gacagcgtgg
     6421 acttgataag ggacagccta ttctctattc aagtggaaca gccgtggcta ctcttacagt
     6481 ttggaaacag caatgcagaa gaaatcggga cagaccgtgt agaagctctt gtatcggcaa
     6541 gcccagagga cgaggacggg aaaaccagag aggaagtcgt aaaaacagaa atcgaggata
     6601 acgagaacaa caatctgacc ataccgcagg tggtaaatcg tttaggtatg gtgttcttcc
     6661 tcttgttttt caacttgggt atcacgatat ttgtattctt gcttacgggc atgatgttat
     6721 ttagccagat actttttatt attttcgcta tgttcctgcc tattagtttc ctgctctcta
     6781 tgataccgag ctatgaaagc atggcaaagc aggcaatcgt gagggtattt aataccataa
     6841 tgacaagggc aggaattacg cttattgtaa cggtggcgtt cagtatttcc agtatgtttt
     6901 ataacatttc cacagactat ccgttcttta tggtggcgtt cttgcagata gtgtgttttg
     6961 ctggtattta tatgaagtta ggtgacttaa tgagtatgtt ctctttaaat gccagcgaca
     7021 gtcaaagcat gggacgaaga attttcagca gaccgtatct ctatatggca catagggcta
     7081 ggcgtatgga aagacggctt gctagtgctg ttaccgctgg cggtgttgct ggtgcggtgg
     7141 ctggtagtac agtttctaat gcacggacaa caaggaacaa ttcagcaaaa gataaccgag
     7201 gcaatacata ttccagcatg ggacaacgtg caggctcaaa ggtgggtgct gtcttagata
     7261 cgaaaaataa agtcaaggac aaagcaaacg ccatgaaaga aacggtcaaa gatatgccga
     7321 cacagaccgc ttatgcggtg tattccgcaa aggaaaaagc aaaaacaagt gtatcggact
     7381 tcaagcgtgg catggtgcag gaacagcagt ccagacagtc gggacgcttg gaaaagcagg
     7441 aacagcataa gaagaatatc gctgataagc gtatggagct tcaaaaagca caagaagcaa
     7501 ggcaggcacg aaagacggac ggatcagcga catcgggagc tacccgtccc catgaacgac
     7561 cagccacagc tccaacttct aaaacaagca agccagtcaa aataaattct gcgaaaaaac
     7621 gtccgctatc ttcgggggtt tctgatgtga aagctccaca atcggcacag ccagtacata
     7681 gacagaatgt agaaaaagtt cccgtatcag agaaaacacg tcagaatttc aaggcagagc
     7741 gtacaacaaa ggcacatatc gtccagcaac caaagaaaat aacggaaaca actgaaaaga
     7801 accgtaacgc aatatttacg aaaggacaga agaaaaaatg aaactgaaac atatcgctct
     7861 tatcggcagt ctgtttccta tcctcttttc catagtcctt ttctttgggg tactgatgag
     7921 tgcggacagc gacgacgaga acagcaactt ttcttcgggc attacgggta tgaatttatc
     7981 cgcagaagtc ttgaaacatc agcctatggt ggaaaagtac gctaaagaat atggtatttc
     8041 tgagtatgtg aatgtgttac tggctatcat tcaagtagaa agtggcggta cggcaaaaga
     8101 tgtcatgcag agttcagaaa gtcttggttt accgccaaac tcgttagata cagaaagctc
     8161 aatcaagcag gggtgtaaat attttgcgtc cctgctttct tcctgcaaaa atcaaggaat
     8221 aaatgatttg aatgtggcga tacagtccta taactatggc ggtggctatg tgggatatgt
     8281 ggcaggaaaa ggaaagaaac atacttttaa ccttgcagag agcttcgccc gtgagaaatc
     8341 gggcggtaaa aaagtaacct acaccaaccc gatagccgtt gcaaagaacg ggggctggcg
     8401 ttatggctat ggaaatatgt tctatgtgga attagtcaat cagtatttaa ctgttccgca
     8461 ggtatcggga gaactggcac aaaaggtaat gaatgaagca ttgaaatatc aagggtggaa
     8521 atatgtttat ggtggtagca atcccaacac ttcctttgac tgttcggggc ttgtgcaatg
     8581 gtgctatggg aaagctggta tttcattacc gagaaccgcc gacttgcagt atgacgcaac
     8641 ccagcatatc ccattatcgc aggcaaaggc tggggacttg gtatttttcc attctaccta
     8701 taacgctggt tcgtatgtta cccatgtggg aatatatgta ggaaataatc agatgtacca
     8761 tgcaggcgac ccgataggat atgcagactt aaatagtagt tactggaaac agcacttaat
     8821 tggtgcagga cgagtaaaac aatagaaagg acttgaaaat atgtttaaga agaataagaa
     8881 aacgacagaa aatgtcaaag aaaagaaagt gcgtaccgtg aaagtgggta cacataagaa
     8941 aactgtgatt gcattgtggg tggtgcttat cgcaagcgtg agttttggcg tgtataagaa
     9001 ttttaccgct attgatatgc acaccgtaca tgaaacagaa accatacaat taagacttac
     9061 tgataccaac gggattgaaa atttcgtgaa gaactttgcg aagtcctatt acacatggaa
     9121 taacagcaaa gaagcgattg aagcaaggac gcaggcaatc agcgattatc tgacaaagga
     9181 attgcaggac ttgaatgttg ataccattag aaccgacata ccgaccagct ctgccgttac
     9241 tgatgtaatt gtatggagta tcgagcagtt gggaacggac actttttctg ctacctatga
     9301 agtagatcag cagataaaag agggagaaca gacaacagca gtcaaggcaa cttatactgt
     9361 aaaagtccat gtagacgctg acggggatat ggtaatcata cagaacccta cccttgcacc
     9421 agcaatcgaa aaatcagagt atgagccaaa gacactagaa acagacgcaa gtgtggacgc
     9481 tgatactgtc aatgacgcta ccgcttttct ggaaacattc tttaatctgt acccgacagc
     9541 aacagaaaaa gagcttgcct actatgtagc tagaaatgtg cttgaaccta tcggcaagga
     9601 ctatctttat tctgaattgg taaaccctat ctttataaag gacggggaca atgtgaaagt
     9661 caaggttgct gtgaaattcc ttgataatca gacaaaggca actttagttt cgcagtatga
     9721 gcttgtgcta cataaggata gtaactggaa aattgtagga taacatggaa atgggcttgt
     9781 atcataatta tggtatgcaa gcccttttta tattgaagta taaattaaca ataattataa
     9841 ttaggtacat gaatggaggg ataaaatgtt caaaattggt gagttttcaa aattgacaca
     9901 agtttctact cgtatgttgc gttattatga tgaaacaggt ttattaaaac ctgctaaaat
     9961 tgattcatta actggatatc gtatgtattc agcagaacaa atctcaactt taaatagaat
    10021 tatatattta cgtgatagtg gtttcaatgt cgctgaaatt gcattagccc tcgattgcag
    10081 taatgatacc attatagaac agcttgataa aaagtctatt gaaattatgg ataatataca
    10141 aaaagaacaa gaaaaactaa agaaaattga gattgctaaa aaagaattgt tgcatggcaa
    10201 aagtgaattg tattttaaca taaccataaa agagataccg agttattgcg ttttatcttt
    10261 aagaaaagaa ataccaaatt attattatga gggcgaactc tggaaagaac tctctgcttt
    10321 tgctaaagaa aataaaataa atatttctga tgaaacattc tcaatatatc acaatgaaga
    10381 atataaagaa aaagatgttg atgtagaatt atgtgccatt gtaaataaaa taggaaaagc
    10441 tacaggagat tttaagtttc gttttaccga accagtttct atgatggctt gtactatggt
    10501 atatggagat ttttctaata tttcttatgc ttatcgttca tttgcaaaat ggcttcaaga
    10561 aaacagtcaa tataagatga aaggttcaag ccgtcaaata gttcatcgtg gaccgtggaa
    10621 cgagaaaaat ccagagaact atctaacaga aatccaaatt cctttagaaa atctcttgta
    10681 aaaaatgata atctgccttg actttaacat ggtgttaagg tttatcgttt atttatcaag
    10741 aagaaaacgg aggaaaatat aatgacaact tataaagtaa attcaatagg tactatctac
    10801 aacaatgaaa aaggtgcttt cattcaacta gaagcaccat atattctagc attaagggca
    10861 ttagacggat tcagtcatat aaatgtttta tggtggttca gtgattttga taatacggat
    10921 tttagaagtg tattggaaac agaacagcct tataaaaatg ctccagagaa aatggggatt
    10981 tttgcaacaa ggtcgccagt acgtccaaat ccgattgctt tatcagtagt agaaattatc
    11041 aatatcgatt atatgaatgg aatgatacaa attgcttaca ttgatgctaa tgacaaaacg
    11101 cctatcttgg atattaaacc atacacaccg agtttggata gggtggaaaa tccaaatgtt
    11161 ccagattggt gtagtcattg gcctcatagt ttagaagaat ccgcttcttt tgcatgggaa
    11221 aacgaattta atttttaagg agctaactat ataaaaatca agtggttttt tataaaaata
    11281 gaaatttagt tagtaaaagt atgtaccttg aaaatatgtg aataattatg gattttgtgg
    11341 cacaacaaat agacctttca aagaaggctc gttgtatcag ttggggtgta tttatggcta
    11401 tatcagtaac gcaggatcaa aggtctgtcc ggtacttaaa agatgataga tgattcttaa
    11461 gagttttctg atacagtgcc cctgagcaca tctgtgacct ttgccttcgg ctaacttttt
    11521 gttgtaataa gcagtaaaga ctctgttgtg attgataacc ggcaaaataa tctgatacag
    11581 cgtttttctg agataccggg aacctttctt ggttattgca gtatgttggg cggtaaactg
    11641 gctggattca tagtgatatg gggcaacacc tgcaaatttg attatctgag acgcctttgt
    11701 atagttgcaa atatctccta attccgctaa aatggaagta ccagagaagt gtgaaattcc
    11761 tggtatggag aggataggag agttgttttg caaggagaac tcttctatct ttttgtctat
    11821 ttcagaaagt tgttcttcta tcatttcaat ttgacataca aggtgtctaa tctgaatttc
    11881 ttcagcaaca gaaggtatgc cgacagaagc tttggctact gttttgagct gctcggcaga
    11941 aagtgggatt cgttttccac gcccctcata ttcaaaacat ttccgaatga ttcgaatatc
    12001 ggagtttgca atcttttcag cagaagcaaa tgttttcaaa acattcatgt aaacaatgcc
    12061 atactttgaa cgaaataagg aattaaattc aggaaatacg atatcaatgc atttctgaag
    12121 ccggtttttg tagatgttta attcctcttt caggttgtga tgatggcgtg tcagctgctt
    12181 ttgttcataa aggtcgaaac ggtttacttt tgtaatgcga taaggctttt tacgctgatt
    12241 agagccgatg acatcacaaa tagtaagaga atcaagagga tcgtttttcg taatgccgcc
    12301 ctgaagtttt ctggtaaggt cagtagttgt tgggttgatc agagcaaccg tatagtgacg
    12361 ttcaagaaga tatttgagta aggcaaagtg ataatgtccg gtatcttcca tgccaataag
    12421 aagttctgat ttgacatagc tgcttagttt tccgatgagt aacaggaatc ctttttgatt
    12481 gttattaaaa acagaaggat ttgatagtat ttctccagtt cctttgtcaa tgatggagaa
    12541 agtgtgttgt tttttaccga tgtcaattcc gactaaaatc atgataatac attcctttcg
    12601 taaaaaatat gtaatgtgtt atccacagca cttacttcgt aaaacctggt acgagatacg
    12661 aattcaagac tcatctaact aatcattatt tagaaataag gtgtggtgag aacctcctta
    12721 aagtagtcag agccacagga tgaaaatgta caaatccaca tcacatattt atatgttaca
    12781 aatgtacaac accgtaatca accacggatc aaaaggttga tagaaaggag aaggtatatc
    12841 tgtaaatgac tattagatga gtcaattaca atataccagt gatgaaccat gagtttgcag
    12901 gattacatta ttgaaaaacc agtaattgat acagaacacc ttattttaag aacaatggat
    12961 aaaaatgaca tagatgattt gaaagaatgg attagtgata tttctttata cgagtattgg
    13021 gggaaacgtc cttcaaaaag cgaaaaaaat ccagaagtac tctttagtaa gccagaaaaa
    13081 gctactaaaa gttttcattg gagaattgtc cataaacaag atagcaaagt gattggtgaa
    13141 atatggatat accttattga aaataataga atggcaaaag tggctttcag actttctaag
    13201 caatatcaag gtaatggact aatgagtgaa gcgttgaaaa gcataattga attttgtttt
    13261 tcaaaaacag aattacaaaa aatatggaca gatgtgcata ttttaaatca tgcttcttac
    13321 aagacattgg agaaagtcgg ttttcaaagg gaaggacata ttcgaggggg aaaaatggta
    13381 aatacctatt gcgactacta cctatatgga attttaaaat ctgattatat cactagctga
    13441 tgttatggct aatattgaag cgacgattgg cgaagcaatg aacaatacag ttccaaaggt
    13501 ctatgatacg aaaagcatta aaagatatgt gatgaattta ttagatatgg ctcattaaaa
    13561 agtaccaaag aaataaaagt taccatatag acaaaattgg ttttttatat cttttgattt
    13621 cttataagat aatggcattg tgcgacaagc tatacaaaaa attttcatgt atactttgat
    13681 tataaattat acaaggaggt actgatatgt acgcatatga aaatattgaa atgtcattaa
    13741 actatattga acaacattta agtgagaaaa tcgaaacaga gaaactggcg gaaattgcct
    13801 gtctttctac attttattac caacgcttgt ttaaaaagtt agttaagaaa tcagttcaag
    13861 agtatatcaa acttcgccgt ttagcaatgg taatagcaga attgaataat tcagaggaaa
    13921 gaatattaga catagcttta aaatatggat tttcagacca tgctaacttc acaagagcat
    13981 ttaaagaagt ttaccatatt attcccgatg aatacagaaa gaatgttcct atgttaaata
    14041 catttgacaa gccagaactt tcttcacgtt atatcatgat tgatgaaaat gtcccattaa
    14101 ttgtaggaaa tattgttttg gaaattcaaa gaaaaacatt aagaacaaag gaaacatatt
    14161 ttggttttga aaaaacagta aatattgctg aacaaattcc tgctggagaa agtacaggaa
    14221 ttgatgttcc aggtcagcta tggcgcgaat tccatgcaaa gaaactgcaa accgaaaatg
    14281 ccatttttaa caatgatatt gaacttggtg tatctcactt agcaagtcca gataaaggaa
    14341 cattccttta ctttgcagga ggtatggttt catcaacaga tgtatgtcct aaaaatatgg
    14401 tacaacatac attaccagca ggtgagtata ttgtatgcaa aattgaggca gaatcttttg
    14461 aagaacttgt tactactgct ttaaatcaag caaataagta tctgtttgaa acttggcttc
    14521 caaatcataa tttggttaca aagccattca tggcagaaaa atattacaaa ggaaatgcag
    14581 agattaatta tatggaagta tggataattc ctgtgtaaac agtaaatgct aaataggaat
    14641 tatctgacgt aataaaaagg aaatagaaag cttataaagg agggcatagt ccgttgtgat
    14701 aaacataaaa gatataaatt atgtacctaa tattagtgaa ataagcgttt atattgaaaa
    14761 ctctgcattt gatgagttgt atcagcatat gaacaatgaa tataaagcag tttgcaaaat
    14821 cgaatacagt aaagatgtct gggcacgagg ttggaacgta aagtttaaaa aatctgggaa
    14881 atcattgtgt gttgtctatc cgaagaagaa ctactttact gtattggtgg ttgtaggtaa
    14941 taaagagaaa gaagatgttg aaaacttgtt acctaggctt tctgaatatg tacaggaaat
    15001 ttatcgtaac actagagagg gaaatggaca aaggtggctt atgatagact taaaatctaa
    15061 aaataatgta tatcaagatg tattaaggct tattgaaatt cgttatgcca taaaatgtgt
    15121 aaaaccgtaa taaaaatatt tttcacatac gtaatctcaa ggtattatcg ttgagacaag
    15181 taacctatat aattatggga aatgagattt ttagatactt aagaaagtac tttagggggc
    15241 ttgcgttatg gaaagattta agtacaattc cacacaatca aatacaatct aataaagctt
    15301 tactaaaaat tgaatgcaat acagctttaa aggctcgtac aaactatatg ttttgtgcgg
    15361 gcttttttca tttaatagga atttggattc gtcagagagt atattataaa aataaaacga
    15421 taagggtacc ttaaaaataa ggtacctcag gctgtagaca aagtcccgga ctctcgtctg
    15481 ggcttttctt atatttgagt tatccacaat tcaacttttt aagaattatc ttattaagaa
    15541 aaaagtgtat gttttccacc cgatagtttt acttctgtga tatttgcaga tgccattcaa
    15601 gtagaaagtg gtggtacggc agaagatgtc atgcagagtt cagaaagtct gggactaccg
    15661 cccaactctt tagatacgga aagctcaatc aagcaggggt gtaagtattt tgcgtccctg
    15721 ctttcttcct gcaaaaatca aggtatcgac gatttgaatg tagcgataca atcttacaat
    15781 tatggcgggg gttatgtggg ctatgtggca ggaaaaggaa agaaacatac ttttaacctt
    15841 gcagagagct tcgcccgtga gaaatcgggc ggtaaaaaag taacctacac caacccgata
    15901 gccgttgcaa agaacggggg ctggcgttat ggctatggaa atatgttcta tgtggaatta
    15961 gtcaatcagt atttaactgt tccgcaggtg tcgggggaac tggcacaaaa ggtaatgaat
    16021 gaagcgttga agtatcaagg gtggaaatat gtgtttgggg gaagcaatcc caacacttcc
    16081 tttgattgta gcggacttgt gcaatggtgc tatggaaaag ctggtatcta cttaccgaga
    16141 acggcacaaa cacagtatga cgcaacccag catatccctt tatcgcaggc aaaagctggg
    16201 gagttggtct ttttccattc aacctataac gctggttcgt atgtaacgca cgtcggtatc
    16261 tatgtgggaa ataatcagat gtaccatgca ggcaatcagc ggttatctaa caaagaaatt
    16321 gcaggactta aatgttgata ctattagaac cgttatactg actagcccca cagttacaga
    16381 tgtgattgtg tggaatatcg aacagtcggg aacgaacact ttttctgcta cgtacgaagt
    16441 agatcagcag ataaaagagg gagaacagac aacaacagtc aaggcaacct atacggtaaa
    16501 agtccatgta gacgctgaca gggatatggt aattattcag aaccctaccc ttgcaccagc
    16561 aatcgaaaaa tcagactatg aaccaaggac accagaagca gacggtaaac tggacgctga
    16621 taccgtaaat gacgcaacca catttctgga aacattcttt aatctgtacc cgacagcaac
    16681 agaaaaagag cttgcctact atgtagctag aaatgtgctt gaacctatcg gcaaggacta
    16741 tctttattct gaattggtaa accctatctt tataaaggac ggggacaatg tgaaagtcaa
    16801 ggttgctgtg aaattccttg ataatcagac aaaggcaact ttagtttcgc agtatgagct
    16861 tgtactatat aaggatagta actggaagat tattatgtaa gacattttgt tggcaggctc
    16921 tattttcgga tatagtctgc cttttctgta gcaaatgtac tgcactgaca agctatttca
    16981 ttgtatttta agcaatggaa tctttcttga caattttacc ttaaatcata tatttatagc
    17041 tattgacttt ctgcttgcaa taagctataa tatgactatg aaatcaaatc agtcatatag
    17101 gaggtatggt gtatgtataa agaagttggc atttcttcaa ataatgttcc gataacactt
    17161 tctatttggt atacagaaag aagtagtcct accattgttt ttttgccagg tactatgtca
    17221 caccctctca tgtatgagaa ttttctatgt ggattgagcg aaagagggtt taatataata
    17281 ggtgttcact atctttcaca cggaaaaagc cctaaaatca aaaaaaatta tataatggaa
    17341 gatatgttgc ataatgtata tgacgcaaca acttacggaa tagaaacttt cggtgaaaac
    17401 atagctgtca tgggttccag tcaaggcggg attttagctg caatggcggc aggtagggac
    17461 acaaggttaa aagccgtttt tccacataat atcatgctga ctacattaaa agaaactatg
    17521 agcttaacaa aatatcccgc atggtcgcac cgttttatgg ggctgataca gtgggggttt
    17581 cgtgtaggtg gaaaattgct gcctaactac aaggttccag gagacgctta tttagactat
    17641 gacagagtat tttatagtga acaggcaaaa caagattgct taaatgaccc tatgctttta
    17701 ccctattatc ctttaagatt tgtcgccagc ttatttaatg ctgacttatc gtgcctggag
    17761 aatggggcta tacaatgtcc tgttatttta attactgcca aaggtgaccc gctttttagc
    17821 tttgattata ccaacaaagt atttgattta attcatgtac cgcaaaagga attacttgct
    17881 ttagagctta atcgtcacat gatttttaat gaggacacgg acactacaat aaacgcattg
    17941 gaagttacct tgtataaata tcttggatag catattgatt atataatcag taaatgttat
    18001 acttttcatg acgatataaa tattttagtc ataaggaggt tatgagtttg ccacgattta
    18061 cggaacaaga aaaagaaatc ataaacagta aacttttgat agagggagaa aagctgtttg
    18121 cattacacgg cttaaaaaaa gtaacagttg atgatttagt agctgctgta aacatatcaa
    18181 aaggttcttt ctatgctttt tatcccagta aagaacatct atatgttgaa atcaattttc
    18241 gcttacaaaa agagttattt acaagtatag aaacaaccat taaaaagaaa aagtataaga
    18301 accatagaga tttagcaaag gacgttatca tgctaagttt aacgggggtg attacttcgc
    18361 ctattctttc acagattgat ttatctttaa tggactatct gcaaagaaaa atatcgtcag
    18421 atatttttga aaatcatatg tacagtgata ttcgtatatt ggagatattg gaagatttag
    18481 gggttgtatt tttggttccg catacggtta ttataaaatc cctgtattct gtcttatcct
    18541 gtttggaaca attcaaagag gacgaagaac tgaatatgat acaaaatctt ttagtgaatg
    18601 gtattattca gcaagtagta gcagagtaac aattttgttt ggttatttga caggcaggta
    18661 gccaaacaaa ttttttacag tttatatgac tatatatcta taaacgtcat aaagttaaag
    18721 gaggaatgaa taatgattga tgtaaaaaaa ctgtatttta gctatactga caaaccgttt
    18781 gtggagaatg taagtttcca tgttgggaga ggagaaattt ttggtttttt aggaccctct
    18841 ggtgctggaa aatcaactat tcaaaaagtt ttaactgggc ttaatacaag atataaaggc
    18901 agcgtaaaag ttgcaggtac agaaataagg gagcatacaa atcagtttta tgaaaatatt
    18961 ggggtagact ttgaattttc aacctgctac gagaaattca cagcacgaca aaatctcgcc
    19021 tattttgctt ctctatatga aaagcaacct cggtctattg atgaattatt gcatatggta
    19081 ggcttggaaa atgacgggga caaaaaagtt gctgactttt ccaaaggtat gcgttctcgc
    19141 ttgaatttca ttaaagcgtt agtccatgac cctgatatat tattccttga tgaacctaca
    19201 agcggtcttg accctacaaa tagtcgttta atgaaagata ttattcttgc ggaaaagaaa
    19261 cgcggaaaaa cgattatcat tactactcac aatatgtttg acgcaacaga gctatgtgac
    19321 caagtagcct ttatcgttgc cggaaaagtc agtgcattgg atagcccgca taatttgatt
    19381 atgtcaaggg gagctgccaa aatacaatat acctactatg ataaaggcga gaaaaccgga
    19441 gaatgtctgc ttgatagaac cacagaagat aagttgttaa aaagtttaat ttccaaaaac
    19501 cgtttgcttt ctattcatag tagtgagcct acattaaatg atatttttgt tgatattaca
    19561 gggaggacac ttcaatgaga ttgggacgtt taatttgtgg tgatatacat ttccaatgga
    19621 aatatggttt ctattttatc tactttatat taacagtttt atatgtgtgc gggattgccg
    19681 ctttgccgga acattggaaa acggatattg cttctattat gatctactct gacccagcag
    19741 caatgggatt gtttttcatg ggagcgattg ttcttttgga aaaaagtcaa aaggtattaa
    19801 atgctatggt ggtatcccct gtaaaaatat cggaatatat actttcaaaa acagttgcgt
    19861 tgattgctat ttctacggta atcgcattga tattaggggt agtttccggc agcaatcatt
    19921 tactgggtat cgcagttggt acggctttga cctcagcaat ctttactatg cttggaatta
    19981 ttgcagctac aaaaatttca aacttgaacc aatttctcat tgtgattatg ccgatagaaa
    20041 ttgtttgctt tgtaccacct attgttggtc tgtttgtgaa gttaccatat ctgttccgct
    20101 tttttccatt cacagcttgc atgaacctta taactggaaa aagcgtttta ctatcgtttg
    20161 atatggtact tgtaattgct acgttgatta tcctctacat agttgcgcga catacagtta
    20221 agcatatgtg gaaaagttta ggaggtgtaa agctatgaaa aggatattat ccagtaccat
    20281 acaggtattt agacaaatca agagcgaccc tatgatgttt gcagcttgtt ttacgccttt
    20341 tgtcatggga gctttgatta aattcggtat tccctttctt gaaagaataa cagatttttc
    20401 tttgcaagcg tattatccga tctttgattt attactttct atcatggctc ctgtattgct
    20461 ctgctttgca tttgcaatga ttacattaga agaaattgac gataaagtgt cacggtactt
    20521 ttcgattacc cctcttggta agtcggggta tctttttaca agattgggag taccctcaat
    20581 tatttcggcg gtaatcgctt ttattgtact gttgcttttt tcattagaaa agttgtccat
    20641 tggaatgaca atatgtttgg cgcttctcgg ctcagtacag gcaattattg tttcacttat
    20701 gattattaca ttatcaagca ataaattaga gggtatggca gttacaaaac tagctgcact
    20761 tacattactc ggaatacctg tccccttcct tattgatagt tactaccagt tcgcggttgg
    20821 cttccttcca tcattttggg tagcgaaagc catgcagaat gaagcggttc tttatttctc
    20881 cataggattg gtggtagctt tagtctggta ctacttcctt acaaaacgtc tgtttcggaa
    20941 gctggcagga taaggaggcg tattatggaa aagggtagac ttctgcaaaa caaaaatata
    21001 aataaggcta ttcaggtttc caaaatcaca ctaatcatca acctgagttt atcgctactt
    21061 aaatttgctg ccggatatat aggaaagtca agtgccatgc tgtcggacgc tgtacactcg
    21121 gcttctgatg tattaagcac aatcgttgtg atggttggaa ttaaaatatc agaaaaacag
    21181 cctgatagag aacatcctta tgggcatgaa cgtatggaat gtgtggcttc cattattctt
    21241 tctgtcgcat tggctataac aggtgctgga attggatatt caggtatcaa gaaaatcttt
    21301 tcagaacagt acaatacgct ttctgctcct agtggaatcg cacttacggc ggcagtctta
    21361 tccattgtta taaaagagtg gatgtactgg tttacaagaa gtgctgcaaa gcatacaaat
    21421 tctgacgcac ttatggctga cgcatggcac caccgctctg atgcactctc gtcggttggt
    21481 tccttaattg gcattttagg agcacgttta ggctatgcta ttctcgaccc tattgcaagc
    21541 gttgtgattt gcggttgtat cttaaaagct gctttggaca ttttcaaaga aagcattaac
    21601 aaaatggtag accatagttg tgacaatgct actgaaacaa agattaggga agttgtgctt
    21661 cagcagcaag gggttgatgg tattgatgaa ttaaaaaccc gcatgtttgg agcgaaaatg
    21721 tatgtagata ttgaaatatt agctgatggt aatttagctc tttatgacgc tcatagaatt
    21781 gctgagggag tccatcaaac gattgaaaac aactttccac aatgtaagca ttgtatggta
    21841 catgttaata cgaatgaact tctctattct gcataggaca gggaagatga tttaaggcta
    21901 ttgaaaggga aaggagggaa gtaggttatg gaacaatact ggtggattat cattgttgtg
    21961 gttttgattg tcgttggctt acttaaaatt gtaattatga agaagtataa ccaaaggaga
    22021 aattctaaca atcgttcaaa agatagcgag gacgacaaga tgtattaagg tttattgaaa
    22081 tctgttatgt tgtagaatga tgtttttaga tactagaaag tgctttaagg ggggctaacg
    22141 ttaaggaaag ctttgagtac aattctttac aatcaacagt cttttgcata gagaaatgta
    22201 tgtaggaaat atggtgcaag gcacaaaaca ccagcaattg agaaatcaac ctatgaacca
    22261 aaggcacact acattttata ctacttgaca agtaaacgaa cgtttactat aatggtttaa
    22321 agaagtaaac gttcgtttac taaagaagga ggttgtaaat gtctgacaca aaagaaaata
    22381 tattaaaaat tgcactacac ttatttgcag tgaatggtta tgaggctgtt tctgttagta
    22441 tgattgcgga agccttggga atgacaaaag gagcattata taaacattat aaaaataagc
    22501 aagatatttt tgactgtatc gtagaacgta tgtatcaaat tgataataag cgagctaagg
    22561 attatcaagt accagaaagc acaatagagg taaccccaaa tgcatacaga aatacaacta
    22621 ttggaagtct aataacattt actcttgcac aatacgattt ttgggtgact gatgaatttg
    22681 cgtctaattt tcgcaaaatg ttaatactag agcaatatag aaatttaaaa atgaatgtgc
    22741 tttatcaaaa atgtcttgta aatggtccta tcagttatat agaagatata tttaatgaaa
    22801 tgattaaaaa aggtgttctt caagagtgtg atacaaaaca gcttgctatt gaatattatg
    22861 cgccattatt tctattgata agtgtagcag acgcagttga aaattgttgg gatttaaaaa
    22921 ctcaattaac tacacacgtt aaacaatttg tcaagaaata tattataaat gagaaagtaa
    22981 aataatcctt tattctgtcc taaatataaa cctaactttt taatttcagc agagaaactc
    23041 aaaatgaata ttgttataag tcacaaatat taactattaa aatattacaa taacggagat
    23101 gagaaaatgg aatatattcg cagtaaaaaa tattttacac ccgacttaca gacaaaaatt
    23161 atggggccga atcctataaa attacaagag gaactattac aaaatcataa aatcccgtct
    23221 ggtagctatg tctgtgacct tggaagtgga caaggtttaa cttctgtatt tcttgcaaaa
    23281 gaatatggat ttgttgtttt tgcagttgac ttatggagtg agccaaaaga aaatgaagaa
    23341 tttttcaaaa aaatgggatt atcttctgca caaattaaat ctgtaaaagc tgacgccaat
    23401 gaacttaaat ttgagaaaga gttttttgat gctgtagtaa gtacagattc atataattat
    23461 tttggacgtg accctatata tttagatgaa aaattgcttc catttgttaa gaaaggtggg
    23521 tttgtttata tcacagttcc tggcatgaag aaagattgcc atgagaaact tccagaagag
    23581 ttattgttat catggacacc agaagaactt gattatatac atgatataac atattggtct
    23641 aacataatta gtcattgtaa aaaggcagat ttaatttcaa tatatgaaat ggaaagtaat
    23701 tcggaagttt ggtcggattg gataaagcaa aataatgaat atgcaataaa tgaccgtaag
    23761 gcaatagaag ctggcggtgg agattatctg aattttattg ctataattct acaaaaaaaa
    23821 taaattcttt agaaccaaac actataattt gcgttctaat atgtttaccg attgacatca
    23881 ataattttat ctttgagggt aatgatttga aaatagaaga ataattccag tttgtaaaag
    23941 tcaataaatc caaagataaa tttgtatttg aaaggagttt tgaagaatga aaaaatatga
    24001 gtatgtagaa attaactata atgcgaaagg agttgtattc ctttgtactg ataaacacaa
    24061 agaaattatt gacagttacg caaaaacggg gtatcactac gcagggtttg tacccacaga
    24121 aattgacgca aaaggctgta tgcgaaagat agatttaatt tttgaaaaag aagattaact
    24181 tattttaggg agttggttct aatgatagga cttaaaagac gagatatgaa ttatattatg
    24241 aaagttggtg ctaaagctca aggtgtttct gtatctgaat ttagggctat gctacaagat
    24301 accatagaca aaactatgga aagtacagac ccagacgtgc aggcaaattt taaacgatac
    24361 tttggaaata agcgcccaac accagaggaa tatatttata cgattaccaa aaagacaaag
    24421 gttaaggttt aaatgttgta aagggggctt gccgtatgaa acgctttatg tacaattcag
    24481 tatattcaag cccaatttaa cagcatacta tttacaatta aatacgctat accagtagct
    24541 cgtacaaaat ttatattttg tgcgggcttt tttaataccc gaaaagtttt tttaattatt
    24601 ttcatttttt tggaagcaaa tcacaccttg ctgtacagat atggtgcgga aagagggata
    24661 aacacttttc aattcatagc tggaaaggag gtgagattat gaaaccgtct gacttccaaa
    24721 aaacaattca gtgtcagttt gaatgtaagc tgaaaaaagt tgtcaaaggt attatctgga
    24781 actaccgtaa agaattaaaa cgaagaaaaa acaaggaaat atcttttagc gaattaccag
    24841 acattacggt agaaggtctg gcaacatggg acgactacga aacaaactac acaattttta
    24901 atgtgtgtgg catagatatt aaagtccttg atgatgaact tgcagaagct ctcaaacagc
    24961 tacccgaaaa aaaacgtaac aatgtattga tgtattactt cttggaaatg agtgatacag
    25021 agattgcaga tattatgaat tataccagag gggctgtgtt caaacacaga caccgtgcac
    25081 tagaaactat gaaagagata ctaaaggaga agaaatagaa atgaagaaaa agtacaagca
    25141 cccgtcttat tatttgattt catcagctat ggatggggat gaaactgcga ttgagaaatt
    25201 actggcattc tatgacccgt acatatcaaa gtgttgttta cgtcctttat atgatgaata
    25261 cggcaatgtt tatattgttg ttgatatgga attaaaggga cgtattcgag aagccttaat
    25321 caaaatgatt ttagactttg agattgatat agatatagaa accgaagaag aataagcaga
    25381 taccagcagg gcgtgattgg ttaaattccc ttctttcctg ccctgctcct gcttttatac
    25441 atggaagcct acggctttcg ccgattgttc ttagacaact gaataaagca gacagatacg
    25501 tttgtgatac agcgagccac ggggactgta cgccatgacc tttccgacaa gaaagcgagc
    25561 gacccacgca gttgatccga gagcgacttg tggaatggtc gttttgccat gacctgctat
    25621 cacagaataa tgatacgtcc gcatggtgcg gttctgccca caagaatggg aatagttgag
    25681 atactaacgg agctttccaa agccgtctgt ctgcttatga taagacacac agaaaggagt
    25741 tgttgcaaca tgaacaatac tgatgtgccg atttgggaaa aatacaccct tagtatagaa
    25801 gaagcgtccc gatattttcg tatcggggaa aagaagttgc gtaaacttgc cgaggaaaat
    25861 ctggactctg gttgggttat cgttaatgga aaccgtattc agataaagcg aaaacagttt
    25921 gaaaaaataa ttgatacact ggacgaaatc tgatgtctta gagccgtaga tatggtataa
    25981 tattagtagc gtatcacggc tcattccatg acggaaagga gctttacact atgtcaaaaa
    26041 tgaaaagaca agacagtaaa ggacgcaatt tgcgtcttgg agagagccag agaaaagacg
    26101 gaagatacgt ttataaatat accgatatat tcgggaagcc acagtttgta tatgcttgga
    26161 aacttgtacc cacagacaaa acacctgctg gaaaacgtga tgatatatcg ttgagggaaa
    26221 aagaaaagca gataaaaaaa gaccttgacg acggtatcga cacagtgggt ggtaaaatga
    26281 cagtctgcca gctttacgaa aagaagaaca atcaaagaaa gaacatcaag agaaacacaa
    26341 aaataggacg acagtatctt atgaacgcac tggaaaatga cccattgggt atgagggcga
    26401 ttgatacggt caagcagtcc gacgctaaag aatgggctat cagaatgaat gacaaaggtt
    26461 attcctacaa aacgatagat aactataagc gttccttaaa ggcttcattc tatatggcga
    26521 tacaagacga ctgtatcaga aaaaatcctt tcaatttcaa gttgagtgat gttctggaag
    26581 atgatatgga agcaaaggtt atcctaacac cagagcagga agaaaaactc cttgccttta
    26641 tggaaacaga taatgtctac agcaagtatc gtgatgaagt tattcttctg ctggaaacgg
    26701 gacttcgtat ctctgaatta tgcggactga caacacacat tgatatgctt aaccgtgtga
    26761 ttaacataga ccaccagcta ttgcgtgata cagaggtggg ttactatatt tccacaccta
    26821 agacaaaaaa cgggaagcgt gagcttccat tgacagaaag agcatatcag gcacttgaac
    26881 gaattttaaa gaaccgagga aaagcacaac cgcttgttgt tgacggttac agcaacttct
    26941 tgttcttgaa ccgtgaggga ttgccaaagg tggcaggaaa ttatgaaagt atggtgcgtg
    27001 ggctgattaa gaagtacaac aagacgcata aggacaagtt gccaaacatc acgccccatt
    27061 cattcagaca tacttactgt acaaacatgg caaataaggg aatgaaccct aacaccctgc
    27121 aatacattat ggggcacgca aacataacca tgacattagg gtattatgct cacggtactt
    27181 tccagtctgc aaaggctgaa ttagaaaagc tggcttctta atatcgaaaa tgtatcttac
    27241 tacttattta ctacttttgg ttgcattttc atgctggtaa atgccatctt atgcgaggta
    27301 tcttccaaaa ggaaaatgcc aataaagccc gaaaatatgg gatatatcgg catttaccaa
    27361 cttatgaaaa gataatcaga aatttagtaa gttttttata taaaatataa agaattatat
    27421 tttattcttt tttatgatgt ctataatctg tgatatcaga tttagttctc ttgaatttaa
    27481 gtcttttaac tcttcattta tatcattttc taaatatgtt tttccagtgg aagtaatatc
    27541 ttttataaga acatctgcag aaatatttaa tatatttaca aattttatga atgttttaag
    27601 acttggattc tttttaccac tttcgatttg agttatatac caaggcgata ctccaaccat
    27661 atttgcaaat tcttttgaag ttaaatttgc ttttttccgt tcttctttaa gtcttttacc
    27721 tatataattc attattgtta ctatttccct cacttatatt tctttgtatt taatgatgca
    27781 ctttattaac tctagcattg caatttgttc tttttcagtt aatttattaa tcatttcata
    27841 acattctaaa ggtacagagt tatcaacgcc ttctgttgta ataatatttg caaataacaa
    27901 ttcaagtggt atgtctaaag cagtagcaat tttcattata atattgatag atgtatttgt
    27961 ctcagctctt tcaatctgac caatatatgt tggatgtaaa cctgcctttt ctgctagaaa
    28021 ctcttgacta tatcccaatt ttgttctata gacttttatt ctatctccta ttatttttcc
    28081 tatatttgcc aattattatc cctccacttt agattatatt catagtttaa gttgttttat
    28141 atagactatt tatacttttt tagtctaaat atagactata tatgttttta tactgaaat
//
LOCUS       DAEMIK010000038        27933 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEMIK010000038 DAEMIK010000000
VERSION     DAEMIK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.038"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..196
                     /locus_tag="KPK87_001411"
     CDS             35..196
                     /locus_tag="KPK87_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537572.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALIAVGLVFNAGGVKDVLL
                     ELFNRIIGA"
     gene            273..509
                     /locus_tag="KPK87_001412"
     CDS             273..509
                     /locus_tag="KPK87_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537573.1"
                     /translation="MKDIFTDMQAKIGCPYLSDLPYYKRAVWFEIKRLCLSDYPKKQL
                     EDFSRYVFGVPYTVIQEALQRKDVMKHGRNACAD"
     gene            484..981
                     /locus_tag="KPK87_001413"
     CDS             484..981
                     /locus_tag="KPK87_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2537574.1"
                     /translation="MEEMRVQIEAREPTDGEIRVFWFTLPIDTEQVEELLGIDIDTDY
                     YYITGKELPFADEVQEDTTIEKLDDLYHTYESLPSDLKEDYGELMCYFTDLDELHRYR
                     NDIIHYSWCKNMTDVARHILNNDPDFTSLSENVTRYFDYEAYGQYLDDNGRFVETDHG
                     IYELP"
     gene            999..1502
                     /locus_tag="KPK87_001414"
     CDS             999..1502
                     /locus_tag="KPK87_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2537575.1"
                     /translation="MVDDMRVYIANLGKYNEGELVGDWFSFPIDEEDVAERIGLNSYY
                     EEYAVHDTDNFPIEIGEYISIEELNEMYDMICELPDYITDALDEFVSYYGSLEEVYEY
                     KDEIYYYPNCEDMTDVAYYFIDELQVLGQIPLPLQNYIDYEAYGRDLSIEGTFIETSR
                     GICEIPY"
     gene            1612..2001
                     /locus_tag="KPK87_001415"
     CDS             1612..2001
                     /locus_tag="KPK87_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537576.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFTLPFPVTFTQITWFVITEFAV
                     ILLGDLPPLSLIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKVTY
                     AGKAVKLEKEVFSEPVTAVRSVMYVPD"
     gene            1988..4435
                     /locus_tag="KPK87_001416"
     CDS             1988..4435
                     /locus_tag="KPK87_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2537577.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSVRSIQEQSKKLVTGRLRDVAIQKIDEQTEALVSMI
                     GDNQVDYRFFIGFKLIVTEEKVSLDSMKKSAFMTFKEFLHEVNHTLMNDFISMPDDEI
                     NRYMKMEKLLENKISRRFKFRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLSKKKLRKA
                     TLIKQYDLIRPTRCLIEESQRYLRLEHEDSESFVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVGNRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLIVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKENAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIDELRREDTAIARNIADHIDSFTDYDFAHLLFSDGSV
                     SNAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVKSEVE"
     gene            4435..6567
                     /locus_tag="KPK87_001417"
     CDS             4435..6567
                     /locus_tag="KPK87_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2537578.1"
                     /translation="MKDKIKGLLTRKKLFRFFKIAVIVVLTTIALLSLLGTVAHASGL
                     VDDTVNADNLYSQYPLENYQLDFYVDNSWGWLPWNWLDGIGKSVQYGLYCITNFIWTI
                     SLYLSNATGYVVQQAYKLDFINDMADSIGKSIQTLAGVTENGFGSTGFYVGFLLLIIL
                     IVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINDFSSDISTAS
                     LDLGTKIMLPDSDSAGKDSVDLIRDSLFSIQVQQPWLLLQFGNSDIEEIGADRVEALV
                     SASPSAGDGATREDVVKTEIEDNDNDNLTIPQVINRLGMVFFLLIFNLGITIFVFLLT
                     GMMIFSQILFIIFAMFLPISFLLSMIPSYENMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMIAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YMFMRHRARRMEHRIARAVGAGSVAGAVAGTVAGTTMNDGSGKSARAGTGRKERRENA
                     SFGSRAGSTVGAVLDTKNKVKDKATAVKENIKDMPTQTAYALHSAKEKAKENVSDFKR
                     GIVQGQETRQSQRADKREQHRQNIADKRMELQKAQEARQTGRTTDGSATTGATRPHDR
                     PVTASKTDTGKVQEIKRPVTTDKVHTEPTKAQPVKERPLASKSAEPVLQKHGTRLNGV
                     ELNQQTTRKERNIKKATLNMTGKKGRKK"
     gene            6564..7151
                     /locus_tag="KPK87_001418"
                     /pseudo
     CDS             6564..7151
                     /locus_tag="KPK87_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7865..9730
                     /gene="ltrA"
                     /locus_tag="KPK87_001419"
     CDS             7865..9730
                     /gene="ltrA"
                     /locus_tag="KPK87_001419"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2537579.1"
                     /translation="MNRVKTSKTLRHSEYYDMTETFDRLYEASQNQQIFVDIMSVIMS
                     DENIMLAYRNIKKNKGSNTKGTDKQTIQNIELMSEKEFVQKIKDMFCCYTPKPVKRVN
                     IPKPNGKLRPLGIPTIWDRIIQQCVLQVLEPICEAKFHNNSYGFRPNRSAHHAIAQCY
                     RLMNQSKLQYVVDIDIKSFFDNVNHAKLIRQMWTMGIRDKHLLGIIRAMLHAPIILTD
                     GKIEHPKKGTPQGGILSPLLSNIVLNELDWWIASQWEEIPTKSKSARVLDRRDKGKGI
                     DKGNKYKELRKSTLKEIYIVRYADDFKIFCRKRKDADKIFHAVIQWLEQRLSLDISEE
                     KSKIVNLRKQKSEFLGFEMKLVRKADKYVVQSHMSKKAMNKAENNLKEQIKRIQHPAN
                     DREQAKEISIYNSMVVGIHNYYNKATLIAFDCSKVNYIISNWIDNRLNVTKQGAIQNK
                     YLSKKYGKSQQIRWLNNCPIIPIGYVKHSKAISIKNEICKFTPKGRELIHKSLDIDVG
                     VLVQLMKNPILDMSVEYNDNRISKYTAQLGRCAVLGVSLSFQEIHCHHIKPISDGGTD
                     KFQNLIIVHESIHKLIHATSQEIISKITNKFKLNKKQISKINALRIKVNNGVITV"
     gene            9825..10160
                     /locus_tag="KPK87_001420"
                     /pseudo
     CDS             9825..10160
                     /locus_tag="KPK87_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            10816..12612
                     /locus_tag="KPK87_001421"
     CDS             10816..12612
                     /locus_tag="KPK87_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2537580.1"
                     /translation="MKQTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPVRSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIVNQKIKDARFLKLIRLFLKAGYMEDWKYHETYSGCPQGGIISP
                     ILANIYLNELDRHIMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKTATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTGIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTDSDRYEIHHVNKVKNLKG
                     KSEWEKVMIAKRRKTIVVCHKCHMAIHHGEKK"
     gene            12730..12837
                     /locus_tag="KPK87_001422"
                     /pseudo
     CDS             12730..12837
                     /locus_tag="KPK87_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010989669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
     gene            12862..13755
                     /locus_tag="KPK87_001423"
     CDS             12862..13755
                     /locus_tag="KPK87_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537581.1"
                     /translation="MFKKKENPIKEKKIRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDQHTTHEVETIQLRLNDTNGIENFVKNFCKSYYTWSNSKEAIEARTQAINAYLT
                     QELQDLNVDTVRTDIPTSSTVTDVLIWDVEQSGENAFSVTYEVDQQIKEGDQTRNVSE
                     TYTVTVHVDADGDMVIIQNPTLAPAMEKSSYEPKAQEADNSVDADTVNDATAFLETFF
                     KLYPTATDKELAYYVEGNALEPINGDYLYSELVNPVFTADGENVRVSVAVKFIDNQTK
                     ATQVSQYELTLHKDSNWKIIG"
     gene            13867..14478
                     /locus_tag="KPK87_001424"
     CDS             13867..14478
                     /locus_tag="KPK87_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2537582.1"
                     /translation="MNQNKENSKKTFDKQAISYDTDMVGEHARNLYAPILKILKQEHH
                     VGSLLDLGCGTGALLESIFNLNITRQLSGIDLSPNMIEEAKKKIGDNVKLYIGDAEKL
                     PFEDGLFDTVICNDSFHHYPAPNRVIKEVSRVLKKGGLFVIGDCWQPAGARQIMNFYM
                     KHSKSGDVKIYSKKEMENLLSKDFCTVLWERISNTSSLTIARK"
     gene            14542..15102
                     /locus_tag="KPK87_001425"
     CDS             14542..15102
                     /locus_tag="KPK87_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2537583.1"
                     /translation="MRISKEPEERKQEILETAMKLFAQNGFEKTSISDIAKEIGVAQG
                     LCYRYFPSKDVLFQTALNEYANILVSKMTKNINIKQDTLKEILNKMTLFSEHESDLYY
                     QVFHDKQNKNFHDLLVLCVCKKLTPIVQDIIIRANQNHEINISDPETCASFCVFGQLG
                     ILMNNEIAFSEKSDRIKTFFNNLFNF"
     gene            15179..16495
                     /locus_tag="KPK87_001426"
     CDS             15179..16495
                     /locus_tag="KPK87_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2537584.1"
                     /translation="MNSKIDFINGNTKKCLLSMTLPMIVAMFLNMAYNLVDSLWIGNL
                     LGEQAYAALTNSTPIILILTSIGMGATNGVSILLSQAIGAKEETKKKRVLSTSFFVSV
                     IFSILITILLEVFLNPILTALNTPDEIFDMARSYLSIYILGYVAVYLYLYFTAILRSF
                     GNTMFQAVAMLVSTLLNAILDPILIKIIGFHGAAIATLTSQIICLIFMCIYISKKKLF
                     KFTLSDFDKLEIMPLIKNAVPSVIQQSIPAISTTFLTALVSGYGISAIAGYGVTGKLE
                     TILFYPAMALNMVLTSIVGQCIGGKRIDRAKDYLKLSLKYGSITLIILSLIIIVFSKQ
                     LAGLFVASENVAVIVNQYFTIVSIGYVLYTITSCCLGTLNGLGKPTRSMILMIFYYIV
                     IRIPLAYIFANLGSGLVGIWLAILISHIVACIASIICGIGSMKIKE"
     gene            16500..16724
                     /locus_tag="KPK87_001427"
     CDS             16500..16724
                     /locus_tag="KPK87_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537585.1"
                     /translation="MIGLKRYDMKALLKAGAKARGLSLPEFRAEIQKNIDNAMNSQDP
                     VVQKHFKKYFGNKIPTPEEYIYTITKKTKI"
     gene            complement(16794..17156)
                     /locus_tag="KPK87_001428"
     CDS             complement(16794..17156)
                     /locus_tag="KPK87_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537586.1"
                     /translation="MRKKKDTHSFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGKHPSFQLFHDLVTMFHISVDEYFYPQEKAEKSTIRRQIDVSLDELTDEELK
                     IIQGTIDGIMKYKGTGSK"
     gene            17683..18105
                     /locus_tag="KPK87_001429"
     CDS             17683..18105
                     /locus_tag="KPK87_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2537587.1"
                     /translation="MKPSDFQKTIQCQFDCKLKRTVKGVIWNYKKELKRRKDKEIPFC
                     ELPEIVVEKLAVWDEYMSDYTVFEVCGVEIRVLDEELSEALKQLSERNRENLLMYYFL
                     EMSDTEIAKAQKISRAGVFKNRYRALQNLKKTLTKERE"
     gene            18109..18357
                     /locus_tag="KPK87_001430"
     CDS             18109..18357
                     /locus_tag="KPK87_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2537588.1"
                     /translation="MKKGYKLPPYRLISSAVDGNEQSIERILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREAIIKMILGFDIGTVPIEE"
     gene            18728..18931
                     /locus_tag="KPK87_001431"
     CDS             18728..18931
                     /locus_tag="KPK87_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2537589.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGENKLRRLAEENIGSNWVI
                     MNGNRMQIKRKQFEKILDKLDAI"
     gene            19010..20206
                     /locus_tag="KPK87_001432"
     CDS             19010..20206
                     /locus_tag="KPK87_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2537590.1"
                     /translation="MAKEVRRDNKGRILRTGESQRADGRYLYKCTNALGESVSLYSWK
                     LVPTDKIPKGKRKDISLREKIEQLQKDLNDGIDPAGKKITVCQLYDKKNSLRKNVKHN
                     TVKGRKQFMNLLKKDPLGSMTIDTVKLSDAKEWAIRMSENGVAYSSIRNYKRSLKASF
                     YLAIQDDYIRKNPFDFKLSDVLDDDTEEKVVLTPEQEASLLSFMQSDKTYRKYYDEVV
                     ILLETGLRISEFCGLDLNVAVDMRNRSILVEHQLLKDKEIGYYISTPKTKNGSRQVPM
                     SKKAYEAFKRVIKNREKGISFTVDGYSRFLFLNQKGLPKVAGNYDGMLRGLVKKYNKT
                     HDEPLPHITPHTFRHTFCTNMANKGMNPNTLQYIMGHANITMTLNYYAHGSYASARSE
                     MDRLCA"
     gene            complement(20446..21111)
                     /locus_tag="KPK87_001433"
     CDS             complement(20446..21111)
                     /locus_tag="KPK87_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG2537591.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            complement(21111..21767)
                     /locus_tag="KPK87_001434"
     CDS             complement(21111..21767)
                     /locus_tag="KPK87_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG2537592.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            complement(21776..22954)
                     /locus_tag="KPK87_001435"
     CDS             complement(21776..22954)
                     /locus_tag="KPK87_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG2537593.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            23309..24211
                     /locus_tag="KPK87_001436"
     CDS             23309..24211
                     /locus_tag="KPK87_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2537594.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(24427..25239)
                     /locus_tag="KPK87_001437"
     CDS             complement(24427..25239)
                     /locus_tag="KPK87_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537595.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(25242..26201)
                     /locus_tag="KPK87_001438"
     CDS             complement(25242..26201)
                     /locus_tag="KPK87_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537596.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(26307..27866)
                     /locus_tag="KPK87_001439"
     CDS             complement(26307..27866)
                     /locus_tag="KPK87_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2537597.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSNGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
BASE COUNT         9143 a         5118 c         5602 g         8070 t
ORIGIN      
        1 ctggttttta agcaacgccc agagcttggt gcttatggca atcgtggtaa tcggtatcta
       61 cttaggattt aagagagagt tttcaaaact tatcggcttc ttggtagttg ccttaatcgc
      121 cgtcgggctg gtattcaatg caggcggtgt caaggacgtg ctgttagagc tgttcaaccg
      181 tatcattgga gcgtagccta tcgttaaaag cggttactcc attggagatc aaccactttt
      241 tctatactca aatttcagat tggagtgatt gattgaaaga tatttttaca gatatgcagg
      301 caaaaatcgg ctgtccgtat ctctccgacc tgccctacta caagcgtgcg gtctggtttg
      361 agataaaacg cctttgcctg tctgactatc caaagaaaca gttggaagat ttttcacgct
      421 atgtattcgg tgtgccttac accgtcatac aggaagcgtt acaaagaaag gacgtgatga
      481 aacatggaag aaatgcgtgt gcagattgaa gcaagagaac cgacagacgg agaaatccga
      541 gtcttttggt tcaccctgcc tatcgacaca gaacaggtag aagaactgct ggggattgat
      601 atagatacgg actattacta tatcacggga aaggagcttc cctttgctga tgaagtgcag
      661 gaagatacca ccattgaaaa actcgacgac ctgtaccata cctatgaaag tctaccctct
      721 gacttgaaag aggactacgg ggagctgatg tgctatttca ccgaccttga cgaactgcac
      781 cgatacagaa acgacatcat tcattattcg tggtgtaaga acatgacgga cgttgcccgc
      841 cacattctga acaatgaccc agactttact tcccttagtg agaacgtcac ccgctatttc
      901 gattatgaag cctacgggca gtatcttgac gacaacggac gctttgtgga aaccgaccac
      961 ggtatctatg aacttccata gaaaagaggt gtaagcctgt ggtggacgac atgagggtgt
     1021 atatcgctaa tcttggcaag tacaacgagg gcgagcttgt cggggactgg ttttctttcc
     1081 ctatcgacga ggaagatgtt gcggagcgta tcgggctgaa cagctattat gaggaatacg
     1141 ctgtccatga taccgacaac ttccctattg aaatcgggga gtatatctcc attgaagaac
     1201 tcaatgaaat gtacgacatg atttgtgagc ttcccgacta tatcacggac gctctggacg
     1261 agtttgtttc ctactatgga agtctggaag aagtttacga gtataaggac gagatttatt
     1321 attatcctaa ttgcgaggat atgaccgacg ttgcctatta cttcattgac gagttacaag
     1381 tgctgggaca gataccgcta ccgttgcaga actatatcga ctatgaagca tacggcaggg
     1441 acttgtccat agaggggacg ttcattgaaa caagccgtgg gatatgcgag ataccatatt
     1501 agagctggca gatcagcgac aggaagcgtc gctactgaca gcttttttct atcaaggaca
     1561 gtctgaaaaa tggctgtcct tttccattac aacgaaagga gctgaaacga attgaagaag
     1621 attaagagct acacaggtat ctggaacgtg gaaaaagtgc tgtacgcaat caatgacttt
     1681 actctcccgt ttcccgttac ctttacccag ataacgtggt ttgtcattac ggagtttgcc
     1741 gtcattctct taggggactt accgccgtta tcgcttatcg agggggcatt tttaaaatac
     1801 ttcggtattc ccgtcgccct cacatggttt atgagccaaa agacctttga cggcaagaag
     1861 ccttacagct tcttgaaatc gcagattacg tttgccctgc gaccaaaagt gacctatgca
     1921 ggaaaagccg tgaagctgga aaaggaagtg ttcagcgaac ccgtcacggc ggttaggagt
     1981 gtgatgtatg ttcccgatta aatatatcga caacaacctt gtctggaaca aggacaatga
     2041 ggtattcgcc tactatgagc tgataccgta taactattct tttctgtcag cagagcagaa
     2101 atttatcgtc catgacagct tccgtcagct tatcgcacag agccgtgagg ggaagataca
     2161 cgccttgcag atagcgacgg aaagctccgt cagaagcata caggaacagt caaaaaagct
     2221 ggtgacgggg cgacttcgtg atgtggcgat acagaagata gacgaacaga cagaagcgtt
     2281 agtaagcatg attggggaca atcaagtgga ctaccgcttt tttatcggct tcaagctcat
     2341 tgtgacagag gaaaaggtca gccttgacag catgaagaaa tcggcgttta tgacgttcaa
     2401 agagttcttg catgaggtca accatacgct gatgaacgac tttatctcca tgccagatga
     2461 tgaaatcaac cgctacatga aaatggaaaa actgctggaa aataaaatct cccgccgttt
     2521 caagttccga cgcttggata agaacgactt cgggtatctt atcgagcata tctacggcag
     2581 ggacggcgtg gcgtatgagg actacgaata ttcgctttca aagaagaaac tgagaaaggc
     2641 aacgctcatc aagcagtatg accttatccg tccgacccgc tgtctgatag aggaaagcca
     2701 gcgatacttg cgtttggaac atgaggacag cgagagcttc gtgtcctact ttaccgtcaa
     2761 tgcgattgtg ggcgagctgg atttcccgtc gtcggaaatc ttctacttcc agcaacagca
     2821 gttcaccttt cccgtagaca cttccatgaa tgtggaaatc gtcggcaacc gcaaggcact
     2881 ttccacggtg agaaataaaa agaaagagct gaaagatttg gacaaccacg cctatcagtc
     2941 tgggagtgaa accagctcaa acgtggtgga cgcattagac agcgtggacg agctggaaac
     3001 cgacctcgac cagagcaagg aaagcatgta taagctctcc tatgtgataa gggtatcagc
     3061 tcccgacctc gacgagctga aacgacgctg tgatgaggtc aaggacttct acgacgattt
     3121 aaacgtcaag ctagtgcgac ctgctgggga tatgctgggg cttcactctg aatttctccc
     3181 cgccagcaag cgttacatca atgactatgt gcagtatgtg aaatcggact tcttggcggg
     3241 gcttggcttc ggggcgaccc agcagttagg agaaaacaca ggtatctata ttggctattc
     3301 tgtggacaca ggaagaaacg tctatttaca gccctctctt gcttcacagg gtatcaaggg
     3361 gacagtcacc aacgctctgg cttccgcttt tgtcggttcg ctgggcggtg gaaagtcgtt
     3421 ctgcaacaat ctgattgttt actattctgt cctgttcggc gggcaggcgg tcattctcga
     3481 ccctaaatca gagcgtggca actggaaaga aacactccca gagatagccc atgaaatcaa
     3541 tatcgtcaac cttaccagcg ataaggaaaa cgctgggctt cttgacccat tcgtgattat
     3601 gaagaatgta aaagacgctg aaagtctggc gatagatatt ctcacattcc ttacggggat
     3661 ttcctctagg gacggcgaga aattccctgt ccttagaaaa gcggtgcgag ccgtgaccca
     3721 gagcgatcag cgaggactgc tccatgtgat agacgagcta cgcagagagg acacggcgat
     3781 agcccgcaat attgccgacc atatcgacag ctttacggac tatgactttg cacatctgct
     3841 gttctcggac ggttcggtgt ctaacgctat cagcttggac aaccagctca atatcataca
     3901 ggtggcagat ttggtacttc ccgacaagga cacgaccttt gaggaataca cgaccataga
     3961 actgctgtcc gtggctatgc tgattgtcat tagtaccttt gcccttgatt ttatccacag
     4021 cgacaggagc atttttaaaa tcgtagattt agatgaagcg tgggctttcc tcaacgtggc
     4081 acagggcgaa accctctcaa acaagctggt gcgtgcggga cgtgctatgc aggcgggcgt
     4141 gtacttcgtc acgcaatcct ctggggacgt gtccaaagag agcctaaaga acaatatcgg
     4201 gctgaaattc gctttccgct ctacggacat caacgagata aagcagaccc ttgaattttt
     4261 cggtatcgac aaggacgacg agaacaacca gaaaaggctt cgtgatttgg agaacggaca
     4321 atgcctgctt caagacttgt acgggcgtgt gggcgtggtg cagatacacc ctgtctttga
     4381 agaacttctt cacgcctttg ataccagacc gcccgtaaaa agcgaggtgg agtgatgaaa
     4441 gataagataa aggggcttct gacaagaaag aagctctttc gttttttcaa gatagctgtg
     4501 attgtggtac tgacgaccat tgccctgctc tccctattgg ggacggtggc acacgcttct
     4561 gggctggtag atgatacggt caatgcggac aatctgtatt cgcagtaccc tttggagaat
     4621 taccagcttg acttctatgt ggacaatagc tggggctggc ttccgtggaa ctggctggac
     4681 ggtatcggaa agagcgtgca gtatggactt tactgtatca ccaactttat ctggactatc
     4741 agtctgtacc tttccaacgc cacggggtat gtcgtccagc aggcgtataa gctggacttc
     4801 atcaatgaca tggcagacag tatcggaaag agcatacaga ccctagcggg cgtgacggaa
     4861 aacggctttg gaagtacggg cttctatgtg ggcttcctgc ttctcattat ccttatcgtg
     4921 ggtatctatg ttgcctacac gggactgata aagagagaaa ccagcaaggc actccacgcc
     4981 gttatcaact tcgttgtggt gttcgtgctg tccgcttcct ttatcgccta cgctcccgac
     5041 tacatcaaga agataaacga tttttccagt gatataagta ccgcttcgct ggatttgggg
     5101 acaaagataa tgctccccga cagcgacagt gcggggaaag acagtgtgga cttgataagg
     5161 gacagcctgt tttccataca ggtacaacag ccgtggctac tcctgcaatt cggcaacagt
     5221 gatatagagg aaatcggggc tgaccgtgtg gaagctctgg tatcggctag tccctctgcc
     5281 ggcgacgggg cgacccgtga ggacgtggtg aaaacggaaa tcgaggataa cgacaacgac
     5341 aatctgacga taccgcaggt catcaaccgt ctgggaatgg tgttcttcct gctcatcttc
     5401 aatctgggga taacgatatt tgttttcctg ctcacaggca tgatgatatt ctcccagata
     5461 ctttttatca tcttcgctat gttcctgcct atcagctttc ttttaagcat gatacccagc
     5521 tatgagaaca tggcgaaaca ggcaatcgtg cgagtgttca atacgattat gacaagggcg
     5581 ggtattaccc ttattgtcac agtggctttt tctatttcca gtatgttcta caacatatct
     5641 accgattacc cgttctttat gattgcgttc ttgcagatag tatgtttcgc tggtatctac
     5701 atgaagctgg gcgaccttat gagtatgttc tctcttaatg ctggggacag ccagagcatg
     5761 ggacgcagga tattccgtcg cccgtacatg ttcatgcgac acagggctag gcgtatggaa
     5821 caccgtatcg caagggcggt tggtgctgga agtgtcgctg gtgcggtggc tggcacagtg
     5881 gcaggaacaa ccatgaatga cggttctgga aaatctgcca gagctggtac gggaagaaag
     5941 gaacgtcgag aaaatgcttc tttcggaagc cgagcgggtt caaccgtagg tgcggtactt
     6001 gatacgaaaa acaaggtcaa ggacaaggca acggctgtca aggagaacat caaggatatg
     6061 ccgacacaga ccgcctatgc cctgcactcc gctaaggaaa aggcaaagga aaatgtgtct
     6121 gacttcaagc gtggcattgt gcagggacag gaaaccagac agagccagcg tgccgataag
     6181 cgtgagcaac acagacagaa tatcgctgac aagcgtatgg agcttcaaaa ggcacaggaa
     6241 gcaaggcaga ctggaagaac gacggacgga tcagcgacaa caggagctac ccgtccccat
     6301 gacagacctg tcaccgcttc taagaccgac acaggaaaag tgcaggaaat caagcgtcct
     6361 gtcaccacgg acaaggtaca taccgagccg acaaaagcac agcctgtcaa ggagcgtccg
     6421 cttgcttcaa aatctgctga acctgtattg cagaaacacg gaacaaggct caatggtgtg
     6481 gagctgaacc agcagaccac cagaaaagag cgaaacatca agaaagcgac tctaaacatg
     6541 acaggaaaga aaggacgtaa gaaatgaaat taagacatat cgcccttatc ggcagtctgt
     6601 ttccctttct tcttgcgata gtgcttttct ttggtgtgct tatcagtgcc gaggacgacg
     6661 acgggggcgg tggttcttcc tcatgggtaa cgggaatgaa cctgtccgca gaggtattga
     6721 aacatcagcc tatggtagag aaatacgcta aagagtacgg catttccgag tacgtcccct
     6781 atctgctggc aatcatacag gtggaaagtg gcgggacagc cgaggatgtt atgcagagtt
     6841 ccgagagcat gggcttaccg cctaactccc ttgatacaga aagctcaatc aagcaggggt
     6901 gcaagtattt tgcgtccctg ctttcttcag cagaaagtca aggcatagag gatataaacg
     6961 tggtggtgca gtcctataac tatggcggtg gctatatcgg ctatgtggct tccaatggaa
     7021 aaaagcacag cttcacgctg gcagagaatt tcgcccgtga caagtctggc ggaaagaaag
     7081 tcacctatac gaaccctatt gctgtcgccc gcaatggtgg ctggcgttac gggtatggga
     7141 atatgttcta tggtgcgaca cgtttctgac tgaaaaggtc aggcacttaa atctgaaagc
     7201 gtaataatcg ttttccttta aggagaactg ataactcaaa gggtggaatg attgggtaac
     7261 gccttgaaac gccctctctg atactccgat tagcatttat tttatagcag ataagtgtta
     7321 aaagctcggt gaagtcggct gaaagttacc gtaaatatac aaaatggata tacgaaacct
     7381 gttcagaggt ggacggtgta actgataggt cgggagtcta taaactgttc acggtgagaa
     7441 tgaaattact gacgaacttg cgaatgaaag agtctataac atgacggtat agaaatgtat
     7501 cgggttctat aaaacggacg catatcgtgg ttgagtaaaa atcgtagata tgaaaaacca
     7561 tgcagtgtta caggcgttgg agatatgcag gcttaaagca agcacctaaa aacagatgta
     7621 aagatagagt tatcggaacg tggaaagctg acaacgggga gattgctata aatctatgaa
     7681 acggttgcta ggaatacata atagcattta tatcagtgaa agtagtggca cagtacctaa
     7741 gaagccatag aaatatggtg gagggatagc cacaagtcag tctttaaact taactatgct
     7801 atatacttca tagaatcgag taagacaaaa taaacaccaa atcctaatag aaaggactgg
     7861 tgctatgaat agggtaaaaa caagcaaaac acttcgtcat agtgaatatt acgatatgac
     7921 ggaaaccttt gaccgactat atgaagcaag tcaaaatcaa caaattttcg ttgatattat
     7981 gagcgttatc atgtcagatg aaaatatcat gctggcatac agaaacatta aaaagaataa
     8041 agggagtaat actaaaggga cagataaaca gaccatacaa aacatagaac tgatgtctga
     8101 aaaagagttt gttcaaaaaa ttaaagatat gttctgctgt tatactccaa aacctgttaa
     8161 gcgagtgaat atacctaaac caaacggaaa gttacgtccg ttaggaatac cgaccatttg
     8221 ggatagaatt attcagcagt gtgtcctaca agtattagaa cctatttgtg aagcaaaatt
     8281 ccataacaat agctatgggt tcagacccaa tcgctcggct catcacgcca tagctcaatg
     8341 ttacagattg atgaaccaaa gcaaattgca atacgttgtg gatatagaca ttaaatcatt
     8401 ttttgataat gtaaatcatg caaaacttat taggcaaatg tggacaatgg ggatacgaga
     8461 taagcattta ctaggaatta tccgtgccat gcttcatgct cctattattc taacagacgg
     8521 aaaaatagaa cacccaaaga aaggaactcc gcaaggagga atactctcgc ctttactatc
     8581 aaatattgta cttaatgaat tggactggtg gatagcttcg caatgggaag aaattccaac
     8641 caaaagtaaa tcagcacgag tattagatag gcgagataaa ggaaaaggaa ttgacaaagg
     8701 caataagtat aaagaattgc gtaaatcaac cctaaaagaa atttatatag tacgttatgc
     8761 agacgatttt aagatattct gtcgaaagag gaaagacgct gataaaatct ttcatgctgt
     8821 tatacaatgg ttagaacaaa ggctttcttt agatataagt gaggaaaaat caaaaatcgt
     8881 aaatcttagg aaacaaaaat cagagtttct tggatttgag atgaaattgg ttcgcaaagc
     8941 tgataaatat gtggtgcaat cccatatgag taaaaaagct atgaacaaag cggaaaacaa
     9001 tctaaaggag cagattaaac gaatacagca ccctgctaat gacagagaac aagcaaaaga
     9061 aatcagtatc tataactcta tggtagtagg catacataac tactataata aggcaacgct
     9121 catagccttt gactgttcaa aagtcaatta cattataagc aactggattg ataacagatt
     9181 aaatgtaaca aaacaaggag ctattcaaaa caaatatcta tcaaaaaaat atggaaaaag
     9241 tcagcaaata cgctggttaa acaactgtcc tattattccg attggatatg taaaacacag
     9301 caaagctata tctattaaaa atgaaatatg taagtttacg ccgaaaggac gagagctaat
     9361 tcataaatcg cttgatatag atgtaggggt acttgttcaa cttatgaaaa atcccatatt
     9421 agatatgagt gttgaatata atgataaccg aatttctaaa tatacagccc agcttggaag
     9481 atgtgctgta ctaggagttt cactatcctt tcaagaaatc cattgtcatc atataaaacc
     9541 tatttcagac ggtggcacgg ataagtttca aaatctaatt atcgtccatg agtctataca
     9601 caagctgata cacgcaacat cacaagaaat aatcagcaaa ataacaaaca aatttaaact
     9661 caataagaaa caaatatcaa aaatcaatgc gttgagaata aaggtcaaca atggtgttat
     9721 tactgtttaa cttgaaaaat atatagcaag taagaaaaag attgatggaa cgccgtgtgc
     9781 ggtgaaagtc gcacgcacgg tgtggagcgg gggaaaactc ggagattacc tcaaagaatt
     9841 atctatcgct attggagctt gtcagccagt atcttacggt cagtcaagta tcgggcgagc
     9901 tggctcagaa gataatgaat gaagctctga aatatcaagg ctgggactat gtgtacggcg
     9961 ggagcaatcc taacacttct tttgattgtt cggggctggt tcaatggtgc tacggcaagg
    10021 cgggcatttc attaccgaga acggcacagg cacagtatga cgctacccag catattccgc
    10081 tttcacaggc acaggctgga gatttggtgt tcttccacag cacctatgac gcaggaagct
    10141 atgtaacgca cgtcggaata gtgcgtccag agggacgaaa gttagaagta gattaaagaa
    10201 agggggacag aaaataaggc actaccccgt aagataacaa gggaaaccac ctgcctaacc
    10261 gaaaggcgaa agctgtacgg gaaaatagca cggcaggaaa gcagaaagtt gttcaaagac
    10321 aatagagcac gactgaactg caaggttaag tgaatatgag gataaaacta gatttgttga
    10381 atgtgagttc caaacctctg ttacgtgtgg acacaggaaa cttgtctgaa acctgtggta
    10441 tgagtaacgc tgaaaataaa tgcctttcag tgggttatca attaactcat acggtacgct
    10501 ggagaaccag tagtaacgga acgaaagcat atccgacaat tcacactagc ccactacttt
    10561 cgtcaactgg agattaccta aaccagaatg cctacaatgg ctatgcgtaa tgctcttgta
    10621 gtgataaatc acaaggctat gctaagtgag atgacgctga aaatctggca aggtaacgga
    10681 gctttcatag tagtctgaga accctgaaaa gacagggaaa gccgtaaaat cggatacggg
    10741 aaaaccgttc acatggcgaa ggaaagcagt cttagcgaat taaaattaga aattgattag
    10801 ggaggaaaac ctcaaatgaa acaaacaatg gagattttaa ccaaattaca ggaaaattca
    10861 aaaaagaacc atgatgaagt atttacaagg ctctatcggt atttactccg tccagatata
    10921 tactatgttg catatcaaca cctctactcc aacaaaggag caggaacaaa gggtgtaaca
    10981 gacgacacag cggacggttt tagtgaaata tatattgaaa atatcattga agccttaaaa
    11041 aatgaaatgt atcaaccaaa accagtaaga cgtacataca tcaaaaaatc aaatggcaaa
    11101 atgcgtccct taggactgcc tgtctttact gataagctga tacaggaagc aatacgcatg
    11161 atattagaag caatctatga gcctattttc tcagactact ctcacggttt ccgaccagta
    11221 agaagttgtc atactgctct tgctcaaata aaaaaagaat ttacaggagc acgctggttt
    11281 atagagggcg acataaaagg gtgttttgat aacatcaatc atgcggtact tgtggaaatt
    11341 gtcaatcaga aaattaaaga cgcacgtttt ctgaagctca ttcgtttatt cttaaaggca
    11401 ggttatatgg aagattggaa ataccacgaa acatatagcg gttgccctca aggtggtatc
    11461 atttcgccta ttcttgcgaa tatctacctt aacgaattag accgtcacat aatgaagata
    11521 aagaaagagt ttgatgtggc aacaaaggca cgttatacac cagaatatac aaaactggta
    11581 ggactacgcc aaagattaca caacaaaatt aaaaatagca atggtataga aagagaaaag
    11641 ctgattgaag aatataaaac tgcaacagct caaatgctca aactaccagc gaaacaatgt
    11701 gacgacaaga aaataaaata tgtgcgttat gctgatgatt tcctaatagc tgtgaatggc
    11761 aatcggcagg attgtgagaa gattaagcaa gaacttacag aatttataag taccacatta
    11821 aaaatggaac ttagtcaaga aaaaactctg attacacaca gtaatactcc tgctcgtttt
    11881 ctcggatatg atgtcagagt acgccgtgac cagcagatta aaccaaaagg aaaattcaaa
    11941 acccgttcta tgaataacaa ggtggaattg agtattccat tcaaagacaa gatagagaaa
    12001 tttttatttt caaatggaat tgtaaagcag aggtcagaca atgggaaatt agaacctata
    12061 caccgaccac aactgcttaa caggacagat ttggaaatag tgactattta taatgcagaa
    12121 ttaagaggta tctgtaatta ttatggattg gcaagcaatt ttaacaagct aatctatttc
    12181 aattatctta tggaatatag ctgtttgaaa acacttgcag gaaagcaccg ttccaaagtt
    12241 tctaaaatta gagctatgta taaagacggt acaggcaaat gggctattcc ttatgaaact
    12301 aaaacaggaa taaagaaaat gtattttgcg aattatgctg actgcaaagg aaagaagttt
    12361 actgatattg ttccacaaac agcaaaaaat tattcccatg acgtaacaac attagaaagc
    12421 cgattaaagg cgaagatatg tgaagtatgt gggtgtacgg atagcgacag atacgaaatt
    12481 catcatgtaa acaaggttaa aaaccttaaa gggaaatcag aatgggaaaa ggtaatgata
    12541 gccaaaagac gaaaaacaat cgtggtatgc cacaagtgtc acatggctat acatcatggc
    12601 gaaaagaaat aacctgtaat ggagcaaaga tggagagccg tgtgcattga gaaatgcaag
    12661 cacggttcgg ggagaggact gcacaaacct accttagaaa taaggaaagg cggtgctttc
    12721 ctactctact atgtagggaa caatcagatg tatcacgctg gcgacccgat aggctacgct
    12781 gatttgaaca atagctactg gcaacagcac cttatcgggg ctggcagaat taagtaatag
    12841 aaagaaagga atggaaatat catgttcaag aagaaagaaa atccgataaa ggaaaagaaa
    12901 atccgcacta tgaaagtggg gacgcataag aagtccgtta ttgccctctg ggtggtgctt
    12961 atcgcaagcg tgagctttgg tgtgtataag aattttacgg ctattgacca gcacaccacg
    13021 catgaggtag aaaccataca gctacgactt aacgatacca acgggataga aaattttgtg
    13081 aagaatttct gtaaatccta ctacacatgg agcaacagca aagaagcaat cgaagcaagg
    13141 acacaggcta tcaatgccta tctgacacag gaattgcagg atttgaatgt tgatacggta
    13201 agaacggata taccgaccag ctctacggtc acggacgtac ttatctggga cgtggaacag
    13261 tctggggaaa acgcattttc tgttacctac gaagtagatc agcagataaa agagggcgac
    13321 cagacaagga atgtatcgga aacctatacc gtgactgtcc atgtggacgc tgacggggat
    13381 atggtgatta tccagaaccc gacccttgcc cctgctatgg aaaaatccag ctatgaacca
    13441 aaggcacagg aagcggacaa cagcgtggac gctgatacgg tcaatgacgc tacggcgttt
    13501 ttggaaacat ttttcaagct gtaccctact gcaacggaca aggagcttgc ctactatgta
    13561 gagggaaacg cccttgaacc gataaacggg gactatctct attccgagct tgtcaatcct
    13621 gtattcacgg ctgacggcga gaatgtgaga gtgagcgttg cggtgaaatt cattgacaat
    13681 cagacaaagg cgacacaggt atcacaatat gagctgacgc ttcataaaga tagcaactgg
    13741 aagattatag gttaataaat aaataggctt gtcattatat tggcgagcct attttacatg
    13801 agtgaatatt attcattgac aatcgttcat tcgtgctgta taataaccaa tgaaaggagc
    13861 gatagcatga accaaaataa ggaaaattca aaaaaaactt tcgataaaca ggctatttct
    13921 tatgatacgg atatggtagg tgaacatgca agaaatctat atgctccaat tttaaaaata
    13981 ttgaagcaag aacatcatgt tggttcactt cttgatttag ggtgtggaac gggagctttg
    14041 ttagaaagca tatttaacct gaatattaca agacagttat caggaataga cttatctccc
    14101 aatatgattg aagaagccaa aaaaaagatt ggcgataacg tgaaattata cataggagat
    14161 gctgaaaagc taccttttga agatggttta tttgatacgg taatatgtaa tgactcattt
    14221 catcattacc cagcaccgaa tagagtgata aaagaagtat cacgagtatt aaaaaaaggt
    14281 gggcttttcg ttattggtga ctgttggcaa ccagcaggag ctagacaaat tatgaatttt
    14341 tatatgaaac atagtaagag tggtgacgta aaaatatact caaaaaagga aatggaaaat
    14401 ctattatcaa aagatttttg cacagtatta tgggaacgta ttagcaatac ttctagttta
    14461 acaatagcac gaaaatgact gaatattagt cattgacata taatgctctg gaagttatga
    14521 tgaaaaagga ggtgtggaat aatgcgtatt tcaaaagaac cggaagaaag aaaacaagaa
    14581 attttagaaa cggcaatgaa gttatttgca caaaacggtt ttgaaaaaac atctatctct
    14641 gatattgcaa aagaaatagg cgttgctcaa ggactttgtt atagatattt tccgtctaaa
    14701 gatgttttat tccaaacggc gttaaatgag tatgcaaata ttttagtttc taagatgaca
    14761 aaaaacatta acataaagca agatacctta aaagaaattc tcaataaaat gacacttttt
    14821 tcagaacatg aaagtgatct ttattatcaa gtatttcatg acaagcaaaa caaaaatttt
    14881 catgatttgc ttgtcttatg tgtttgtaaa aaattaacac ctattgttca agatataatt
    14941 attcgagcta atcaaaatca tgagataaat atttccgacc cagaaacatg tgctagtttt
    15001 tgtgtttttg gtcagttggg aatattgatg aataatgaaa ttgcttttag tgaaaaaagt
    15061 gacagaataa aaaccttttt taataaccta tttaattttt gatatatccc ctgcttaagg
    15121 cagggaatat ttaaacgcta tatatgaata acattcattc actaaggaga gcttaattat
    15181 gaacagcaag atagatttta ttaacggaaa taccaaaaaa tgtttattat ccatgacgct
    15241 tccaatgatt gtcgctatgt ttttaaatat ggcatacaat ttggtggata gtctttggat
    15301 tggaaactta ttaggtgaac aagcatatgc ggcacttaca aattctacac ctataatctt
    15361 gattttaacc tcaatcggta tgggggcaac aaatggtgtt tctattcttt tatcgcaggc
    15421 aatcggtgca aaagaagaaa caaagaaaaa gcgtgtatta tctacatcgt tttttgtatc
    15481 tgtaatattt tcaatactga ttacaatttt attagaagta tttttaaatc ctattctgac
    15541 tgctttaaat acgccagatg aaatctttga tatggcacga agctacttat ctatctatat
    15601 cttaggttat gtagctgtct atttatattt atatttcaca gcgattttaa gaagttttgg
    15661 aaatacaatg tttcaagctg ttgcaatgct tgtatctacc ctgcttaatg caattttaga
    15721 ccctatactt attaaaataa ttggatttca tggtgcggct attgctacat tgacatcaca
    15781 gattatctgt ttgattttta tgtgtatcta tatttcaaaa aagaaattat tcaagtttac
    15841 tctatctgat tttgataaat tggaaattat gccacttatt aaaaatgctg taccgtctgt
    15901 tatccagcag agtattcccg ctattagcac gacttttctg accgcattag taagcggtta
    15961 tggtatttca gctattgccg gttatggtgt aacgggaaaa ttagaaacca tactattcta
    16021 tccagctatg gcattaaata tggtattgac ttcaatagtt ggacaatgta taggcggaaa
    16081 acgaattgat agagctaaag attatttaaa attatccttg aaatatggaa gtatcacttt
    16141 aattatacta tcactgatta ttattgtttt ctcaaaacaa ttagcagggc tttttgtggc
    16201 aagtgagaat gtagcagtta ttgttaatca gtattttacg atagtcagca taggctatgt
    16261 tctttacaca attacaagct gttgtctagg tacattaaat ggacttggaa aaccaacaag
    16321 aagtatgata cttatgattt tttactatat tgttatccgt attccattag catatatttt
    16381 tgcaaatcta ggaagcggtc tagttgggat ttggttggct attttaatca gccacattgt
    16441 tgcctgcatt gcaagtatta tatgtggtat aggttctatg aaaataaagg agtaaaaaat
    16501 tgattggatt aaaaagatat gatatgaaag cacttttaaa agctggtgca aaagccagag
    16561 gattgtcgtt accagagttt agagccgaga tacagaaaaa cattgacaat gctatgaata
    16621 gtcaagaccc agtggtgcag aaacatttta aaaagtattt tggtaataag atacctacac
    16681 cagaagaata catttacaca atcacaaaga aaactaagat ttaagtgctt atcctgcttc
    16741 ctgcggagca gatcagcagg atagcttatg aaaggaaaaa gaaaacgaga agcctatttg
    16801 cttcccgttc ccttgtattt catgataccg tcaatcgttc cttgaatgat tttgagttct
    16861 tcatctgtca gttcatcaag cgatacgtct atctgtctgc ggattgtaga tttttctgct
    16921 ttctcctgcg gatagaagta ttcatcaaca gagatatgga acattgtcac taaatcatga
    16981 aataactgga agctggggtg tttccctata ttctcaatat ctgcgatatg acgttctccg
    17041 taaaagacct tatcgcctaa gtcattacgg gaaagcccag ctttctctct cgcttctctg
    17101 attgccagtc ccagcggacg gaaatcaaag ctgtgcgtgt ctttcttttt cctcatttta
    17161 aatcacctgt catgagttta tacttcatgg cacatgatta gaatgtgtta aaaacacata
    17221 tattatacgt tagtagttcg tatcaatatt accttggcgg taaataaaaa aaaccgttct
    17281 agggtaatat ctttttccat gtctttcaat ttgattagga cggttttgga taaccagagc
    17341 tgtccttttc ttttgcactc acggtacagc tctgaataag ggggttgtgc tatgattgcg
    17401 tatcagcaaa cacgccagta ttcacaaccg aataaccgcc ttacaatggt tatccagtca
    17461 atacacctta aacatgagtt aaaaattcat atcgcaaaca taacaaaaac agtctgttgg
    17521 tacattcatg tgcttattga ctgtttttgt tatttatgag aagttcaata tttttttgaa
    17581 tttctttctt atacaggtta gcaaatcacc cactcctgta ccgatgtggg gcggaaagag
    17641 ggatagatac cttttacgtc tggacagaga gggggtgaga tgatgaaacc gtctgacttc
    17701 caaaaaacaa tccagtgtca gtttgactgt aaactcaaac gtactgttaa aggcgttatc
    17761 tggaattata aaaaggaatt aaaacgtcgt aaggataaag aaattccttt ctgtgagctt
    17821 ccagaaatcg ttgttgaaaa gctggctgta tgggacgagt acatgagtga ctataccgtg
    17881 tttgaggtat gcggtgttga aatccgagtg cttgatgaag aactctcaga agcattaaag
    17941 caattatcgg aacgcaaccg tgaaaatctt ctgatgtact actttctgga aatgagcgat
    18001 acagaaattg caaaagcaca gaaaatttcc agagcaggag tttttaaaaa ccgttacaga
    18061 gctttacaaa atttaaagaa aacactgaca aaggagcgtg aataaaacat gaaaaaaggg
    18121 tacaagcttc caccttaccg acttatttca tctgctgtgg acgggaatga acagtctatt
    18181 gaaagaatac tggcatttta tgacccgtac atatcaaaat gctgtttgcg tcctctgtat
    18241 gatgaatacg gtaatgttta tatcgtagta gatatggagc ttaaagggcg tatcagagaa
    18301 gctattatca aaatgatttt aggcttcgat attggtactg tgccaataga agaataaagc
    18361 aattatggca gggcatgatt tgttgaaatc ccctctttcc tgtcctgccc tctgctttta
    18421 cacatgagag cctatggctt tcaccactgt tctttgacaa ctgaataaag cagacagata
    18481 cgtttgtgat acagcgagcc acgggaactg tacgccacga cctttccaaa agagagcgag
    18541 cgacccatgc agtgatccga gagcgaccgt tggaaaagtc gctttgccac gacctgtatt
    18601 cacaggataa tgatacgtcc cagaggtggt tttgctcata ggaatgagat tggttgagat
    18661 accagcggag cttttccaga gccgtctgtc tgcttccaaa agacacacag taaagggggt
    18721 gcatattatg aacaatactg atgtgcctat ctgggaaaag tacacgctga cgattgagga
    18781 agcgtcaaaa tacttccgta tcggagagaa taaacttcgc cgtttagctg aggaaaatat
    18841 cggctcaaac tgggtcatta tgaatggaaa ccgtatgcag ataaagcgaa agcaattcga
    18901 gaaaattctt gataagttgg acgcaatctg atgaaagcaa gccttaaata tggtataatt
    18961 atactatcga gtttaaggct tgctcctttt acgaaaggag cttcctgtaa tggcaaaaga
    19021 agtcagacga gataacaaag gacgtatttt acgcacagga gaaagccaga gagccgacgg
    19081 aagatattta tataagtgta ccaacgcatt aggagaatct gtttcgcttt attcatggaa
    19141 actcgttccc acagacaaga taccaaaggg aaaacgtaag gatatttcct tgcgagaaaa
    19201 gattgaacag ttgcagaaag accttaatga cggtatcgac cctgcgggaa agaaaatcac
    19261 ggtatgccag ctttatgaca aaaagaacag ccttagaaag aatgtcaagc acaatacggt
    19321 aaaggggcgt aagcagttta tgaacctgtt gaaaaaagac cctttaggaa gtatgacgat
    19381 tgatacggta aagctgtccg acgccaaaga gtgggcaatc cgtatgagtg aaaatggcgt
    19441 ggcgtacagt tctatccgca attataagcg ctccttgaaa gcgtcgttct atcttgcaat
    19501 acaggacgac tacatcagaa agaacccgtt tgattttaag ttgagcgacg ttctggacga
    19561 cgataccgag gaaaaggtag tgttgacacc agagcaggaa gccagcctat tgtcctttat
    19621 gcagtcggat aagacgtacc gtaagtatta tgatgaggtc gtcatactgc tggaaacagg
    19681 cttgcgtatt tctgaatttt gtggattgga cttaaatgta gctgttgaca tgcgtaacag
    19741 gtcaatcctt gtagaacacc agttgctaaa ggataaggaa atcggttatt acatttccac
    19801 acctaaaaca aagaatggta gccgacaagt gccaatgagc aaaaaggctt atgaagcatt
    19861 taagcgagtt atcaagaacc gtgagaaagg catatcgttt acagtagatg gttacagccg
    19921 tttcctgttc cttaatcaaa aaggattgcc taaagtagct gggaattatg acggtatgtt
    19981 aagaggtctg gtaaagaagt ataacaagac acatgatgaa cctttaccgc acatcacacc
    20041 gcatacattc cgtcacacgt tctgtacgaa tatggcgaat aaaggaatga acccaaatac
    20101 attacagtac atcatgggac acgccaatat caccatgacg ctgaattact acgcacatgg
    20161 ttcttatgct tctgcaaggt ctgaaatgga caggctttgt gcatagtggt aaaaagaata
    20221 tttaccacaa aatttactac ttccgaacgg taaaatacat agagatataa aaatctatgt
    20281 gaagtgcttg accctatgaa aaatgcctta gaagcctata aaataaggct tcaagggcat
    20341 ttaagaagat ttaaaaagat aataggaatt tatgataaga ttttaagcaa tacaaattta
    20401 tatgtaatac gtctaagaga tttttatttt ctagttttaa ttattttaaa caacagatat
    20461 ttttccattt aatgaatctg acaatattaa aggagcttca gtagcagaaa taacatcatc
    20521 taatgtgaaa gctggattta tttctttaag taaaattcca tctgatgtta cttccataac
    20581 tcccatttca gtaactatca aatctacagc atttgttgca gtaagtggaa gagtacaatt
    20641 atttaatatt tttgcagaac cttttgaagt atgttccatt gctacaataa cttttttagc
    20701 tccagttact aggtccatag ctccacccat acctggaacc atcttacctg gaatcatata
    20761 gtttgctata ttgccatatt tgtcaacctg aagtgcgcca agaacagtta tatcaacatg
    20821 acctcctctg attattccaa aagaagtagc actatcaaag aaacatcctc caggaagtat
    20881 agtaactggt tgaccacctg catttacaat agtttcatca cttgaatttc catcttcggc
    20941 tgaacctaaa cctaaaaagc cattttcaga ttgtaagata acgtttacat tatccggaat
    21001 ataatttgct actttggttg gtagacctat tccaaggttt acaacggctc cgtcttttaa
    21061 ttctttagat actctatttg caatatattc ttgcatttct aatttatcca ttataaatta
    21121 cctccttcaa cgatgtaatt cacaaaaata cctggtgtat taacttcatt tgggtcaata
    21181 tcaccaattt caacgatttc atctgcttca actattgtaa ttttggcagc agatgccatt
    21241 aaattattaa aattgttcat agagccttta taaactagat tacctgcttt atcgaccttt
    21301 gatgcaaata gaagagcaac atctgcataa attggttttt caagaaggta ttctttgcta
    21361 tcgacaacaa ttttttgctt tccctcctca acaagagtac caataccagt cggtgtgagt
    21421 atacctccta gaccataacc agctgcacgt atttgttcag caagagttcc ctgaggaact
    21481 aactcaacat ctgtttcatt gttgttcatt tgtcttccag tttctttatt taatcctaca
    21541 tgagaagcaa ttatcttttt aaattgctta tttacaacca ttttgccaac tcctttgtca
    21601 ataaagccag tatcattgca aattagtgtc aaatctttta tatttttact acataaataa
    21661 tcaattaaat tttctggtga accattggcc atgaatccac caaccattat tgtatctcca
    21721 tcttttacat gagatagagc ttcatcaatg ctaactattt tattcatatt tcctcctatc
    21781 gtgaaaccac cattgatatt ccttgaccac caccaataca aagtgttgca agtccagttt
    21841 caactttacg tttttgcatt tcataaatta aggtaacaag tattctagct ccacttgctc
    21901 ctattggatg tcctaatgct attgcaccac cattaacatt taactttgat gaatctattt
    21961 gaagttcatt tttaacagct aatgcttggg cagcaaacgc ttcgtttgct tctattaagt
    22021 ctatatcatt gatgctaagc ccagcttttt tcagtgcttt tctagttgca gggattggac
    22081 cagtacccat gacttcaggt tcaacaccag ctgaagcata ggattttatt ttagcaagtg
    22141 gtctaatacc tagttcgtct gctttttgtt ggctcattag tattaacata gctgcaccat
    22201 cattaatacc tgaggcattt ccagctgtta cagtgccatc ttttttgaat gcaggtttaa
    22261 gtttagctaa accttcaaat gtcataccta atttaggata ttcatcttta tcaatagttt
    22321 ctatttttcc tcgacgtatt aaaacatcta ctggaacgat ttcttcttta aagcgattgt
    22381 tttttatagc attctcagcc ttattttgac tttctaatgc caatttatct tgcatttctc
    22441 tagtaaattc aaatttagtt gcaatatttt ctgcagttat tcccatatga tactgattga
    22501 aagcatcaat aaggccatct gttagcatac tatctaccat ggttatgtca cccattttac
    22561 taccaaagcg agcagttgga acgatataag gagcttggct catattttca gtaccacctg
    22621 caattaccac atcattttca ccagatgtga ttgattgggc agctagctgg acacttttaa
    22681 gccctgaacc acataattta tttactgtgt agcttggaac agaatttggt atacctgcat
    22741 ttatagcaat ttgtctagca acattctgtc caagccctgt ttgaagaaca tttccaatta
    22801 ttacttcatc tatttcagat aaattaagtc ctacacgtga tatcgcttct ttaacagcga
    22861 tagttccaag ttgaacagct gaagtatttt taaatacacc tccaaaactt cctataggtg
    22921 ttctgactgc tgatacgatt actacatctt tcatatctaa tagttacctt tcttgaatga
    22981 taattaaaaa ttttctgaat aaagaaatta gggaacacta taataaagta cttttttaat
    23041 acaagcttgt ttttaagtat ttcaattact tttattccta atgctttagg ttttcctgtt
    23101 tctaattata gattacggcg aaaaaagcgt tttgtctaag tctaattttt tatatagtgt
    23161 ataagaaaaa tttataggat aaaaatttat agtagattta gaaaaatatt ttgttaaaat
    23221 attaatgaaa ttatgttgat tttcctataa aatatcatta gaagaaaaaa tataatctaa
    23281 attatacttt tggggaaggg ggtttaaaat ggatataaaa cagttgaaat attttgttga
    23341 aatagtgaaa tctggtttta atttatcaat agcatctaag acacttcaca tatctcaacc
    23401 agcattaagt caaattataa aaaattttga agaaagtgaa gatgtatatt tatttgaaag
    23461 gtataaaggc aggttaaatg ggttaacacc agcaggagaa agattttatg taaatgcaga
    23521 acatatcata aatgaataca aaaatatgat ggaagattta agagaagatt cagttcaatt
    23581 taaaggaaaa gttagaatag gaatacctcc acttatactt ggaatagcat tttcagatgt
    23641 agttgcacag ttagttgcaa ataatccaga tatagaattt gatatagtag agaagggtgc
    23701 atatgactta aatagaatgc ttatcttaca ggaattagat tatgcagtat tgttacatcc
    23761 acataaaatt gataaaagtg tagtaactga acatgtgctt caagaagatg aacttacagc
    23821 atttttaaat ataaatcatc cacttgctaa gaaggataag atagattgga acgatttgaa
    23881 tgagcagtta cttgcaatat ttaatcctac ttttatgata catcataagc taatgcaaaa
    23941 atttactgat gaaaatgtta agttaaaaag atatataatg tctggttcat gggatttttt
    24001 gttgctatca accacaaatt ctgattttat aacaattcta ccatcaccag ttcatgattt
    24061 ttttgaaaat agcaaaatta tagaaaagcc ttttaatcat cctattacat ggaaagttat
    24121 attatgtaga cctaaaaaag aacgttatag ccatgtagac caacatgtat ttaaatttat
    24181 tatagatttc tttagtaaaa agaactctta aaaatgttat taaaataaca aataaataaa
    24241 tcaattttta ttataataaa ctattagttt ttcatctata tgtttgagag caataatagg
    24301 ctttacataa catggtagag tagtatttaa agttataata tacaaaatat gtattttttt
    24361 aaacgaaaaa taaaaacagt gtatatcttt agataatcac tgtttttatt ttctatataa
    24421 aatttgttaa gtattactag aaaatttttt gcttattata gtcaaactca tgactgttaa
    24481 aaatataagt aatcctggaa atacagctaa atataacttg tcaaaaatat gtctttgagc
    24541 atcttgaagc atattacccc atgatgcaag aggcaattgg attccaagac ctaaaaaact
    24601 aagagcagat tcagttaaga tagcattagc aatatttaac gaaccagcaa ctacaatagt
    24661 ctcttttaag tttaaaacta tatgtcttaa tagtatattt ctagtcttga taccagtagc
    24721 tttagcagct agacagtatt cattttcctt tatttttaat gtttcgccac gtacaattct
    24781 tgaaacttcc atccaactta agaatccaat aattagtata atagtggaaa ctttaggtgt
    24841 tagataagta tttaatacaa tcaaaactaa gaaacttggt atactcataa gcatatccac
    24901 taatctcatc aagaattggt ccagcttacc tccaatatat ccactaataa cacctacagc
    24961 agtaccaaat actacagaaa taagcattga taaaatacca acactaagtg aaatccttcc
    25021 accataaaga gctctagaga agtaatctct accaagttcg tctgtaccaa agatatgctt
    25081 taagctaggt tcttgaagtt tagaagtagt atcaattgca ttagagtcta tatttaatat
    25141 aaaagaagct ataattaata tgattattat gctaagagta atacatgcaa aagcaaagga
    25201 tttatctttt tttaattttt ttaagatact tgtaaacaca actagaatcc tcctttgcta
    25261 gattttattt tagggtcaat taataagtat agtatatcag caactaaatt acctaaaatt
    25321 aaaaatagtg ctgataacat agttattgcc ataattacag tataatctct tgaaaaaatt
    25381 gaatcaatca ttaatcttcc catacctggc caagagaaaa ctacttctgt aacaaatgca
    25441 ccagtgacta atttttgtat actcatgcct aggattgtaa ccaatgatgt cagtgaattt
    25501 ttcaagacat gtttaaatag tatgactttt tctgataatc cttttgctct tgctgtaata
    25561 acatattgtt tttttaattg ttcatttaca ctagcttcta cataatttgt aaaaatagca
    25621 gtattatata gacctaaagt tattacaggc aaaactaaat gttttactaa atctccaata
    25681 ctatcatttc caatagtatg catacctcct gatgggaaaa tgttaagttt aactgaaaaa
    25741 attgaaatga gtatcatacc tatccaaaaa gaaggtatag aaacacctat ataattaaaa
    25801 acagatgata ctttactagt tattgaattt ttatttacag ctaaaaatat tccaagaggt
    25861 atagctacca atattgaaaa tatcaaagaa gtacccataa gaataaatgt atttcccatt
    25921 ttttccataa tttgttctgt aacgggcata tggtttatat aagaaatacc taagtttcca
    25981 cttaaagtgt ttttcaacca gtttaaatat cttatatata ctggtttatc aagtcccata
    26041 ttaaccctta tattttcaat atcctgtgaa gacattaaag gatttatata tgcatctgct
    26101 gggtcaccag gagctaattc taaaagtcca aatgataata tagatattaa gattaacata
    26161 ggtattaaaa cttttactct attcaatatt aattttaaca taagtaaatt ctcctaatta
    26221 aaagagatgt ctcgttttaa aataataaaa caatattatt tttagacatc tccttttcta
    26281 attaataata taaaatttta catcaattat tctacataca gtttagacca atcttcaaat
    26341 aatattgcag gagatggctt agcatctttt attcctttta gatttttttg agctgccatt
    26401 aaattttgtt cataatctat agtgtaaata ggcgcatcct tagatatatc ctgttgtatt
    26461 tgcttatata tttcttcacg tttttccttg tttaactcag taaatccttt ttcccataaa
    26521 gcatctattt cagcattaga atatttagtt tggttgcaat attgaccagt ttcaaacata
    26581 cctctatatg ggtttggttc taatccccaa gcataaccat ttacatacat atcacattca
    26641 gtgctattac taaatagtat attgaagaat gccttacttt cataaggaac tatttcagct
    26701 tctataccaa tttttttcaa ctcttgttga gctactaagg catagttttt atgcccaaat
    26761 ctacctgtgt tataacctat ttttaattta tcaattttaa caccactttt atccaataag
    26821 tctttagcct tatttgtatc ttggtcatat ccttcaacac cttcttctgt atagaagtct
    26881 gcttctggaa ctaatataga tttagcaggt acagaacctt cttttccata tgctgattct
    26941 atcatttcat ttctatttaa ggcatatgat aaggcttgtc ttacttcttt gttagccatg
    27001 tttgatatat tttggttaaa tacaatgtag tttaatcttt cttcactata agtataagtt
    27061 tgtaagtttg agtttgattt tgccttttca tatccttcag cagatgtctt cataagtgaa
    27121 atttcgccat tatttaaagc tgcttcttga gatgcttcat taggaattat ttttagcgct
    27181 atagaatctg ccttaggttc tccaccaaaa taatctgcat tcttttcaaa tactatgctt
    27241 tcaccttttt tccattcctt aaacttgaat gcaccagaac caactggatt attatttttt
    27301 tctgacttag ctatatttga ttctccttca aacacatgtt ttggtattgg agaaatttta
    27361 gatataccat ataagaaaga ttcacttgca gttggtaaag taatcttaac tgttaaatca
    27421 tcaatttttt ctactttaac tggtttctca cccaccaata agtattgtct tgatggtgaa
    27481 ttttgttttt cgtctaaaac tgtattaaat gtaaataaga catcttcagc agttattggc
    27541 tttccatcat gccattttaa attggatttt aattttatag ttaattcctt actatcttta
    27601 aaatctactt tttctgctaa ataatttact aaatttcctt tttcataagt ataaagagga
    27661 gcataaacta tatttgatgc aagagagtct tctttaacgt ttgcaaaagc tccattgatt
    27721 atatctgggt cacttgccat aggtattaca acagttccac cagatttgct tccattagac
    27781 ttgtctttat cattggaagc acaggctgta aatgtaagca ttaatgatga aactaagatt
    27841 aatgatgcca attttttaaa tttcatattc ttataaaacc tccataaaat aatatttaac
    27901 acatattttt tattgtataa tagtaatcat tat
//
LOCUS       DAEMIK010000039        27777 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEMIK010000039 DAEMIK010000000
VERSION     DAEMIK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.039"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..189)
                     /locus_tag="KPK87_001440"
     CDS             complement(<1..189)
                     /locus_tag="KPK87_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2537598.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILIS"
     gene            373..717
                     /locus_tag="KPK87_001441"
     CDS             373..717
                     /locus_tag="KPK87_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG2537599.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            748..2349
                     /locus_tag="KPK87_001442"
     CDS             748..2349
                     /locus_tag="KPK87_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG2537600.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KEFVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2368..3468
                     /gene="dprA"
                     /locus_tag="KPK87_001443"
     CDS             2368..3468
                     /gene="dprA"
                     /locus_tag="KPK87_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG2537601.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YRGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3501..5588
                     /gene="topA"
                     /locus_tag="KPK87_001444"
     CDS             3501..5588
                     /gene="topA"
                     /locus_tag="KPK87_001444"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG2537602.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGKFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5753..6538
                     /gene="codY"
                     /locus_tag="KPK87_001445"
     CDS             5753..6538
                     /gene="codY"
                     /locus_tag="KPK87_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG2537603.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6645..7910
                     /locus_tag="KPK87_001446"
     CDS             6645..7910
                     /locus_tag="KPK87_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG2537604.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7941..8711
                     /locus_tag="KPK87_001447"
     CDS             7941..8711
                     /locus_tag="KPK87_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2537605.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8820..9266
                     /locus_tag="KPK87_001448"
     CDS             8820..9266
                     /locus_tag="KPK87_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG2537606.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9284..10477
                     /gene="nifS"
                     /locus_tag="KPK87_001449"
     CDS             9284..10477
                     /gene="nifS"
                     /locus_tag="KPK87_001449"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG2537607.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10531..10971
                     /gene="nifU"
                     /locus_tag="KPK87_001450"
     CDS             10531..10971
                     /gene="nifU"
                     /locus_tag="KPK87_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG2537608.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11260..12339
                     /gene="mnmA"
                     /locus_tag="KPK87_001451"
     CDS             11260..12339
                     /gene="mnmA"
                     /locus_tag="KPK87_001451"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG2537609.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12482..12720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12788..15427
                     /gene="alaS"
                     /locus_tag="KPK87_001452"
     CDS             12788..15427
                     /gene="alaS"
                     /locus_tag="KPK87_001452"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG2537610.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15452..15709
                     /locus_tag="KPK87_001453"
     CDS             15452..15709
                     /locus_tag="KPK87_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG2537611.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15719..17890
                     /locus_tag="KPK87_001454"
     CDS             15719..17890
                     /locus_tag="KPK87_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537612.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18096..18521
                     /gene="ruvX"
                     /locus_tag="KPK87_001455"
     CDS             18096..18521
                     /gene="ruvX"
                     /locus_tag="KPK87_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG2537613.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18599..18886
                     /locus_tag="KPK87_001456"
     CDS             18599..18886
                     /locus_tag="KPK87_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG2537614.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19168..19626
                     /locus_tag="KPK87_001457"
     CDS             19168..19626
                     /locus_tag="KPK87_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG2537615.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19743..20480)
                     /locus_tag="KPK87_001458"
     CDS             complement(19743..20480)
                     /locus_tag="KPK87_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2537616.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20697..22373
                     /locus_tag="KPK87_001459"
     CDS             20697..22373
                     /locus_tag="KPK87_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG2537617.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22580..22780
                     /locus_tag="KPK87_001460"
     CDS             22580..22780
                     /locus_tag="KPK87_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG2537618.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22834..23997
                     /locus_tag="KPK87_001461"
     CDS             22834..23997
                     /locus_tag="KPK87_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2537619.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24208..24630)
                     /locus_tag="KPK87_001462"
     CDS             complement(24208..24630)
                     /locus_tag="KPK87_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG2537620.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24651..25487)
                     /locus_tag="KPK87_001463"
     CDS             complement(24651..25487)
                     /locus_tag="KPK87_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2537621.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25785..26381
                     /locus_tag="KPK87_001464"
     CDS             25785..26381
                     /locus_tag="KPK87_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG2537622.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            26411..27130
                     /locus_tag="KPK87_001465"
     CDS             26411..27130
                     /locus_tag="KPK87_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG2537623.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27132..27671
                     /gene="scpB"
                     /locus_tag="KPK87_001466"
     CDS             27132..27671
                     /gene="scpB"
                     /locus_tag="KPK87_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG2537624.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11257 a         2904 c         5001 g         8615 t
ORIGIN      
        1 agaaataagt ataaatttat tctcatttga taaagaaaaa aatctaactt gattatttat
       61 tataattaga aataatatta tcatagttat gaccggatac aatgtatttt caacattttt
      121 caaatagctt acaactaata taaataaaga taaatatcgt ataaaagcaa ctaatttttc
      181 tctgttcatt aaaggcactc ctccaaaaaa ataaactttt cctatgaaaa ctagaataat
      241 atcataaaag aataaagtca atagaagtat ttattaatat tgatggaaat aaaatcaatc
      301 caaaaacttg aatagtaaaa ttgtatactg acaataattt aaaagtgaaa atttaatgta
      361 ttggagtgaa taatgaataa taaagaaaaa ggtgattttg gagaaaaagt agcagttaat
      421 tatttgctat caaaaggagc aaagatacta gaaaaaaatt acagattaaa gataggggaa
      481 atagatataa tagcaaaaat ggaagatgaa attatatttg tggaggtgaa atcaaggtct
      541 aacataaagt ttggctaccc ttgtgaatct gtaagcttca aaaaaagaaa aaagataatt
      601 ggagttgcaa gttattatat tataaaaaat aacttaaata acactcccat aagatttgat
      661 gttatagagg tatatttatt agaaaaaaga ataaatcaca taatgaatgc tttttaaaaa
      721 agataatgtt tttggaaagg attaaatatg ttaagtatta ttaattcaag caacttagta
      781 ggaatcgaca gctttttagt taaggttgaa gtagatgtaa gcaatggaat acctagtttt
      841 aatattgttg gtcttccagg caaagaaatt aaagaagcaa gagaaagagt aaagtcagca
      901 attcttaata gtggatataa atttccaagt acgagaatag ttgtaaattt atcaccagca
      961 gatataaaaa aagaaggagc ttttttagat ttatctataa gtataggcct tttaagagag
     1021 ttaataaaaa aagatgaaaa ttacataaga gaaagtatgt ttataggaga attatcatta
     1081 gatgggaaaa taagaaaagt aagaggtata ttgcccataa ttatgggtgc aaaaactcag
     1141 aacataaaac gtatttttat accaattgag aacataaaag aaagtctatt tgtagatgaa
     1201 attgatataa ttcctattaa aagtttaaaa gaatttgtcg attttttaaa cgaagagata
     1261 aaagtcgata aagtaaggat aatgtctttt ttggatgata agtcaagaaa agagaacgaa
     1321 gaattagaaa aagataatag ttatatagat tgcaaatata ctaagattaa taatgaagaa
     1381 agtaaatatg atgaagactt taaagatgta aaaggaaatt attttgtaaa gaggagtgca
     1441 gaaattgctg ctgctggaaa tcataatatg tttatgattg gacctccagg ctcaggaaaa
     1501 actatgattg caaaaagagt aagaacaatt ttaccagata taagtgtaga agaaatgata
     1561 gaagtaagta aggtctatag tatattgggt atgataaatg aaactaaagg aataattgat
     1621 aaaagacctt ttagagcacc tcatcataca actacaaagc aatctttaat tggaggagga
     1681 atggatgcaa gacctggaga aattgcttta gcacatagag gtatcttatt tttagatgag
     1741 atagccgaat ttgatagaaa aatacttgag actttaagac aacctataga agatggatat
     1801 gtaaatatat ccagagtaaa gtacagtgca aaatatcctt gtagagtact gttagttgca
     1861 gctatgaatc catgtccatg tggttattat atgtcagaga cagagtgtag atgtagaagt
     1921 aacgaaattg atagatacat aaataaaata tcaggtccat tgttagatag gtttgatatt
     1981 tttgtagaag ttaattctat aaaatattca gattttaatt ctttaaaaca agaagaaagc
     2041 tctcaaaaaa taaagagaag agtagaaaat gctagaaaaa tacagataaa tagatttaag
     2101 aaggataata taaaaaataa tagtgaaatt aaagcttaca atttatttaa atattgtaaa
     2161 ctggaaaaag aagcttcaaa aacggctgag atgatattta ataaatacaa cctaagcagt
     2221 agaagttata caaaactttt aaaaatggca agaacaatag cagatttaga agaaagagac
     2281 ttgataaatt cacagtgtat tatagaagct ttttcattta gaaaggctta ttatagttat
     2341 tttaaatagt tttttgaggt ggattttatg gaaaaaagag atacttattt atggttaaaa
     2401 tcaattggtg gaataacaac aaaaacaatt gaaataatag aaaatgaaat agtaaatatt
     2461 gaagatatat ttgatttttc tgaaaaagaa atatataatt taaaaaatat aagcttgaat
     2521 attagaaaaa atatagtaaa atatagaggt cacgcttatt tagaaaatat aaaagaacta
     2581 ctttacaaaa aagcgattaa atatatttgt aaatatgata aagaatatcc tgaaaattta
     2641 aagaatatat acaacgctcc aaagttatta ttctacagag gtgacatagg tttagttaat
     2701 aataatttta atatagctat agtaggttca agaaaaccta cagcctatgg aattaattgt
     2761 gcaaaaacaa taagttgtca actatcccaa tatggagtta atatagtaag tggattagct
     2821 ataggaatag atgcatactc gcacataggt tgtatgagtg gaaaatctaa aactatagca
     2881 gtacttggtt caggagtaga caatcctctt ccaaaacaaa atttacattt atcaaataaa
     2941 atattagaaa atgggggatt attattatca gagtataata ttaactcgac agtagccccg
     3001 taccattttt ctaatagaaa tagaattatc agtggtttaa gtgatggagt agttgttgta
     3061 gaagcagcaa taaaaagtgg tgcactaatc acagtagatt ttgctcttga acatggaaaa
     3121 aatgtttttg caatacctgg taatataaat tctcaaatga gtagaggttg tcataaaatt
     3181 ataaaagaag gtgcaaaatt aattgaaaat atagatgata ttttaaatga atataacata
     3241 tttaatatta ttgataaaaa aataaatcaa aaatatgata atataagtct aaatgccaag
     3301 tctaagcaaa taattgaagc tataaaaaga gaaggaaact tgcatataga tagtatttgt
     3361 gattatactg gtatagaaat aaagtatgta aattcaataa tcaatgaatt agtactaaat
     3421 gagttagtag tagaaatgaa taataaaacg tacagtttaa atgtataaat taagattagg
     3481 tagtgggggt gtatggtttt atggccaaaa cattagtcat cgtagaatcg cctgcaaaag
     3541 ctaaaactat agaaaaattt ttagggaaaa gtcattacac agttaaagca tcagttggtc
     3601 atgtcagaga tttacctaaa agtaaattag gtgtagatat agaaaataat tttgaacctc
     3661 aatatataaa tataagagga aaaggcgatg taataaaaga attaaaaaaa gaagctaaaa
     3721 aatctaaaca agtatatcta gcaactgacc ctgatagaga aggtgaagct atatcatggc
     3781 atttagctca tatactaaat ttagatgaat ctgaggattg tagaatagag tttaatgaga
     3841 taactaagga tgcaattaaa aaagcaatta aacatcctag aaatattgat ataaatttgg
     3901 ttgatgcaca acaggcaaga agagtgctag acagactttt aggttatcaa ataagtccta
     3961 tattatggca aaaggttaga aaaggtctta gtgcaggtag agtgcaatct gttacaacaa
     4021 agttaatttg tgatagagaa aaagaaataa aggcatttat tccaaaagaa tactggacaa
     4081 tagatgttga ggctaagaca gtaaaagaca aagatataac tttgaaattt tatggtaaaa
     4141 atgatgaaaa gattgaatta gaaaatgaag aagctgtaaa tgaaatacta aaagaaatgg
     4201 aaggtaaaga tttaaaggtt gagaagatag aatctaaatc aagacgtaaa tcagctccaa
     4261 aaccatttac aacaagtatg cttcaacaag aaggagcaaa taagttattt tttactacta
     4321 aaaaaacaat gatgatagca caagaattat atgaaggtat cgacatagaa ggtgaaggta
     4381 ctgttggtct tatatcatat ataagaactg actctaaaag aatatctgat gaagcaaaag
     4441 aaaaagctaa gggctatatc gtaaacaatt taggagaaaa ctactataaa aacactggtg
     4501 ataaaaaaga aaaaaaggat actaaaaagg ttcaagatgc ccatgaggca ataagaccaa
     4561 cctcagttga tagaactcct gagagtataa aagcctcatt gagcaaagac cagtacaagt
     4621 tatacaattt aatttggaga agatttgtag ctagtcaaat ggaagattca gtgtttgata
     4681 tactaaatgt agaatgtaaa ataggcgata ttgtatttaa ggcaactggt tcaaaaatga
     4741 aatttgatgg ttataccaag gtatataatt tttcagaaag agaagacaaa attttaccaa
     4801 gtatagaaaa aggagatata ttaaatatag aaaatatatt gccagaccaa cattttacac
     4861 aaccaccagc aagatataca gaagctagtt tagtaaaaac actagaagaa ctaggtatag
     4921 gtagaccaag tacgtatgct ccaacaatag ctactatact gaatagagag tatgttgaaa
     4981 agcaaggaac aagtctatgt ccaacagaac ttggaataat agttactgat attttggaaa
     5041 ataatttcca taaatttata gatgtagatt ttactgctga tatggaaagt aaacttgatg
     5101 ttatagaaga aggcaataca gattggaaaa agattgtatc agaatcatat gcaccactta
     5161 aagaggctat tgaagaagct cttgaaaata tagaaaaagt aaatatggat gaagaaactg
     5221 atgagatttg cgagaactgt gggtctaata tggttatcaa atatggtaaa tttggaaagt
     5281 ttatggcttg taaaaactat cccgattgta aaacaactag accaattatg aataaaattg
     5341 gtgttaaatg tccaaaatgt gaagaaggag atattatact tagaaaatct aaaagaggta
     5401 aagcttttta tgggtgttct aattatccaa actgtgactt tgtagcttgg aataagccaa
     5461 caggagagtt ttgtgagaag tgtggttcat atatggttga aaaagtgact aaatctgaaa
     5521 caaaaacagt gtgttctaat aaagaatgta ataaagagac aaaagagcat aatgacggaa
     5581 aagagtgaaa attgaaaaaa atcacataat tctcttgaaa aaggttccaa tctatgataa
     5641 tctttcaagt gtaaacaact aatagaaaaa taaattttca ttaaatgtga aaataatatt
     5701 agaaaatata aaaggttatt aaaaggccta aatttcatga ggagatgatt aaatggcaag
     5761 tgaagtgtta caaaaaacaa ggaaaataaa taaaacatta caaacaagtg gtggaagcag
     5821 tgtctctttt gatttactgg ccggagcatt gggcgacgtt ttaagttcta atgtttatgt
     5881 agtaagtgca aaaggtaaag tactaggtct tcatttaaat gatgttcaag acagttcagt
     5941 tatagaagat gagtatacta agcaaaagaa attttcagat gaatatactc aaaatgtgtt
     6001 aaaaattgat gaaacattag aaaatttaaa tggtgagaag atattagaaa tctttcctga
     6061 agaacatgga agattacaaa aatatactac agtagttcca atattaggaa gcggtcaaag
     6121 attaggaaca ttggtacttt caagatattc aaattcattc aatgatgatg atttagtaat
     6181 agctgaatac agtgcaactg ttgttggtct tgaaatatta agagcaatag gtgaagaatt
     6241 agaagaagaa atgagaaaga aagctgtagt tcaaatggca ataggcactc tgtcctactc
     6301 cgagcttgaa gcagttgaac atatttttgc tgaattggat ggaaaagaag gtttacttgt
     6361 agcaagtaag atagctgata gagttggtat aactaggtct gtaatagtaa atgcacttag
     6421 aaaatttgag agtgcaggtg tgatagaatc aagatcatta ggtatgaaag gtactcatat
     6481 aagaatactt aatgacaaac ttacagatga attaaaaaaa ttaaaaaaca atcaataatc
     6541 tatattttat aggtttagat tagaaaaggt atctatttca aatagatacc ttttttagac
     6601 gaattattaa ggtaatgata agtatattaa aaaggagtga gaaaatgcct ttagcagata
     6661 aaataagacc aaaaacaatg aatgatgtaa taggtcaaag tcatataata ggtaatggaa
     6721 aaatattgtc taagatatta caatctaatt ttttaccaaa tatgatattt tttggtcctc
     6781 ctggtgttgg taaaactaca gtagcggaaa taatagcaga aagaagcaat aaaagcttct
     6841 ataaaataaa tgctactaat tcatctttag aagatataaa aaaggtgata tcagagcttg
     6901 gaagtataaa taatgtaaat ggagtcctat tatatataga tgaaatacaa agttttaata
     6961 aaaaacagca acaatcaata ttagaattta tggaaaatgg gagtataact ttgattgcta
     7021 gcactacaga aaatccatat cattatgtat ataaagctct tttaagtagg tctactgtat
     7081 ttgaatttaa acctttagaa aaacttgata tagaaaaagg actgaaaaga gctgttgaag
     7141 tattaaatga agatagctat atggatattg agtgcaataa taatgctatt gaagatatag
     7201 caattctttc tgatggagat atgagaagag ccttaaatat tttagaagtg gtagtctatt
     7261 caactaaacc aaataaggat aatataactt atatagattc agatgtaata agggcatcta
     7321 catttaataa aattataaac tatgacaaaa atggtgatag tcattatgat attttaagtg
     7381 catttcaaaa atctataaga ggaagtgacc cacaggcatc tatacactat ctagcaagac
     7441 ttataaaagg tggggatttg ataagtatat gtaggagact tttagtaata gctagtgaag
     7501 atataggttt agcttatcca aatgctattg taattgtaaa ggcatgtgta gactcagcta
     7561 tgcaacttgg atttccagaa gcgaaaatac cattagcaga agctactatt cttttggcta
     7621 catctccaaa gtctaattct gcatgtatag caataatgaa agctatggat gaactggata
     7681 aagagtttgt taaggatata ccaaatagta taaaggatgc tcactattct ggttctaaag
     7741 aaataggtag gggttgtgga tacaaatatc ctcataattt taaaaatcat tatgtaaagc
     7801 aacagtattt accagatagc ataaaagata gtatttatta cataccacaa gaaaataaaa
     7861 ctgagaaaaa tataaaaaaa tatttagaat atctaaatag tgatttttag actgaacttt
     7921 atgtatcggg ggtagatatt atgattataa aacttaactc aacttatcac agtagagtta
     7981 tgaaatactt aaaaaaagaa cctgagtaca atttgtttat aataggggat attgaaagat
     8041 atggatatgg aaataatttt ttaaatatat gggcagatgt gggtgaacat ggtgaaataa
     8101 aggcaatatt acttaaatat tttgaattta tgatgtttta ttctgatgga gagtatgatg
     8161 ttgaaggttt ttataatcta cttcgtaata ctaattacga agaaatatct gggaaaatat
     8221 gtgctgtaga tgctttagca aagagattag gacttaataa tttaaaggta gttgattttt
     8281 gcaaacttca aactaaaaag tttttgatag ataataactg taatgcaaag gttaagagaa
     8341 ttaggcttgg aaatctaaaa aagacagtga aactttatga cttaatagat gaatttcaca
     8401 gtacaacttt agagaattta aaaaatggac taaggacagg tagaggatat tgtatagaga
     8461 ttaacaaaca ggtggtgtca atggcaaaga gtacttcaga aaataggact catgcaatga
     8521 taattggagt aggaacacat cctaaatata gagctaaagg acttgctaca aaatgcctaa
     8581 ttaaactatg ttcagagtta ctgagagaaa acaaaattcc ttgtttgttt tatgataatg
     8641 aagaggctgg aaagatatat aaaaaactag gatttgaaaa cattggaaaa tggggaattt
     8701 attcaaaata aaaaattaaa attaatcttg acaaaattag tagggtataa taaaattatc
     8761 ttgaaagtaa ttcctactaa aacagtatga attagatttc tatatagagg tgataatata
     8821 tgaagttgtc tactaaaggt agatatggac taaaagcaat gtttgaatta gcattaaatc
     8881 aagacaatgg accagtatca ttaaaattta ttgctaagaa gcaaaaaata tcagatcagt
     8941 acttagaaca aatattttca tctttaaaaa aatcgggtct agtaaaaagt gtgagaggtg
     9001 ctcaaggagg ttatttgctt tcaaagaatg ctgaagacat aacagtagga gatatacttg
     9061 tagtattaga aggaccagtt gcattatctg actgtgtact agatgaagat gtatgtgaaa
     9121 actcaaacat gtgtgtaaca aaaatagttt gggaaaagat gaaaaagggt atagaagatg
     9181 ttatagattc aattactctt aaagatatga taaatgacta taacaaaaat aaattagaaa
     9241 atgatataac aaatattaaa aaatagattt ggagatgaat gtaatggaaa aaagaagatt
     9301 gtatatggat tattctgcaa caacacctat taaaaaagaa gtattggatg caatgatgcc
     9361 atacctaaca gattattttg gtaatgcatc tagttttcac acattcggaa gagaagcaaa
     9421 agatgctttg gataaagcaa gagaacaagt agcagccctt ataaatgctg aacctagtga
     9481 aatatatttt acagcaggtg gttcagaaag tgataactgg acactagagg gtgtagctta
     9541 tgctaataaa aacaaaggta atcatataat aacttcaaaa atagagcacc atgcaatact
     9601 tcatacatgt gaatacttag caaaacatca tggatttgaa ataacttatt tagatgttga
     9661 tagcgaagga aaagtagact taaagcaact agaagattca ataaaagata ctactatact
     9721 tataagcata atgtttgcaa ataatgaaat aggaactata cagcctatta aagaaatatc
     9781 cgaaatagct aaaaaacata agatattatt ccacactgat gcagtacaag ctacaggtaa
     9841 tattccagta gatgttaaag aattaggaat agatttgatg agtatgtctt cacataaaat
     9901 atatggacca aaaggtgtag gagctttata cataagaaaa ggtgtaagac ttcataactt
     9961 tgtccatggt ggagcacaag aaaaaagcaa aagagctggt acagaaaata tacctgcaat
    10021 agttggatat ggaaaagcag cagaattagc taaggaaaat atgcaaaatc atgtagaaac
    10081 tttaactcgt cttagaaata agttaataga tggtgtgtta gaaagaatac cttacacaag
    10141 agtaaatggt agcttagaaa atagattgcc aggaaatgca aactttgcat tccaatttat
    10201 agaaggtgaa ggaatacttt tattattaga tatgttagga attgctggtt caagtggttc
    10261 tgcatgtact tctggttcat tagacccttc acatgtactt cttgcaatag gtttaccaca
    10321 tgaaatagca catggttcat taagacttac agttggagat tttacaacag atgatgatat
    10381 agattatata ctagagaact taccaaaagt aatagaaaga cttagaagca tgtcaccact
    10441 ttatgatgat gcgaagaaac aaggtttagt aaaatagaca taaagataaa gcataaaaaa
    10501 tagacataaa ttgagtagga ggaaaagata atgcaatata gtgataaagt tatggaacat
    10561 tttatgaatc caagaaatat gggagaaatt gataatgcaa gtggtgtagg tgaagttgga
    10621 aaccctacat gtggagatat aatgaagata tttttagaca tagatggaga tgtaataaag
    10681 gatgttaagt ttaaaacatt tggctgtggt tcagctatag caagttcttc tatggcaact
    10741 gaaatgataa aaggaaaaac tataaaagat gcattagaac ttacaaataa agctgttgca
    10801 gaagctttgg atggtttacc accagtaaag atgcattgtt cagttttagc tgaacaagct
    10861 gtaaaagcag ctcttataga ttatgctcaa aagaacaata tccatatacc agaattggat
    10921 ggatatgtta tagatgatgc tcatgaccat gatgttgaag aagaagaata aaaataagtg
    10981 attgtgttat gagaagttta caatgattaa aaagaagtac taatgatata attaggacat
    11041 aaaaaatagc tgtttttaaa atggctattt tttattgcct aaattaagta ttttaacttg
    11101 atttagaggt taattagtta acaaaataat ccataagatt caaataataa agatgattat
    11161 ttttttagaa aatagaacaa gctatatttt aaataagtac aatacttgct ctatatggta
    11221 taataataag taatgcaaaa gatgaggtga aatttgttta tgaataaaaa agtaatgata
    11281 ggtatgagtg gaggagttga tagctctgta gctgcatatc ttttaaaaca acaaggatat
    11341 gatgtcattg gtgttactat gaagttgtgg caagatgatg atgttgttga aattgaaggt
    11401 ggttgttgtt ctttatctgc tgttgaagat gctagaaggg tagcaaataa aataggtata
    11461 ccattttatg ttttaaattt tagagaagtg tttaaagaaa aagtaataga ttattttata
    11521 gatgaatatt tggaaggaaa aactcctaat ccctgtatag cttgtaataa acatataaaa
    11581 tttgatgatt tttataagaa ggctagacaa ataggttgtg attatgtagc tacaggtcac
    11641 tatgcaaaga tagaaaagga tgaaagtaca ggaagatatt tgctaaaaaa atctgttaca
    11701 gataaaaaag accaaactta tgcactttat aatcttacac aagagcaact agagcatact
    11761 ttactaccaa taggtgacta tgaaaaagat agagtaagag aaattgctaa ggaaatgggt
    11821 atggcagtac acaataagcc tgatagtcaa gaaatttgtt ttgttaaaga taatgactat
    11881 gcaaattatg taaaaaaaca ttctaaaaag cgtattgaag aaggtttttt tgtagatact
    11941 aaaggcaata tacttggaaa acataagggt atattatatt atactatagg ccaaagaaaa
    12001 gggcttggaa ttacttttgg aaaaccaatg tttgtaatag acataaatcc aataaataat
    12061 accatagttc ttggagacaa tgaagattta tttaaaaagg aacttattgc taaagatgtt
    12121 aattttatat caatagatac gttagaagaa cccttaagag ttcaagccaa aataagatat
    12181 tcagcaaaac catcaccagc tactattcat agagtaggag aagatactat taaaatagtt
    12241 tttgatgaag ctcaaagagc tattactaaa ggtcaatctg tagtaatgta tgatggagat
    12301 attgtagtag gtggaggtat aatcgagaaa agtttataaa aacatctaaa aaattaaaaa
    12361 aataagcaat aaagtatatt aagagtaaat aaagtttata aaataaattt attgtatatg
    12421 aaaatatagt attgattaat tttgtaaaat ttagtaatat aattagtata ttaattaaaa
    12481 aacaaataca gtgataagga atagtaaaat atttgatgtc ttacagagag agggaaatgc
    12541 tggaagccct tagaatatga tatttgaagc agccttttag tagcaatctt gaacaattta
    12601 tattttataa agaagattta agtagagatt gacggtagtt acgttatagc taaggttttc
    12661 cgaagatatc atactttgta tgataattag ggtggtaccg cgattgtcct cgcccctata
    12721 aatttattta tagggacgag gttttttatt taaacgaaaa aataaaattt attaggagga
    12781 acatgatatg gaaaagatgg gattaaatga aataagaagt aagtttttag aattttttga
    12841 gtcaaaaggt cattatgtag caaatagtta ttctttagta ccaaacaatg ataagagttt
    12901 gttacttata aactcaggta tggcaccttt gaaaaactac ttttcaggtg tagaagtacc
    12961 tccaagtgtg agaatgtgta catctcaaaa atgtataaga acaggagata tagaaaatgt
    13021 aggtataact gcaagacatg ctacattctt tgaaatgatg ggtaacttct catttggaga
    13081 ttactttaaa agagagtcaa tcaaatgggg atgggaattt gttacagaat ggctaaatat
    13141 accagaagat aagatatggg tgactgtata tgaagaagat gatgattctt atgatatatg
    13201 ggcaaaagaa atgaatttcc cagaagaaag aatggttaga cttggaaaag atgataattt
    13261 ctgggaaata ggtacaggtc cttgtggtcc ttgttcagaa atatattttg atagaggaga
    13321 agaatatgga tgtgacaatc cagactgtaa accaggctgt gattgtgata ggtatttaga
    13381 attttggaac catgtattta ctcaatttga tagagatgaa gaaggaaatt atagtctatt
    13441 agaaaacaag aatatagata caggtatggg tcttgaaaga atgggttgta ttatgcaagg
    13501 cgtagacact atctttgaag ttgatacaat taaatctata ctagaagcag ttgagaagtt
    13561 aacaggagta aaatatggag agaatccaaa aaatgatata tcaataagaa taataactga
    13621 ccatataaga gctgtaacat tcttagttag tgatggagtg cttccatcaa atgaaggaag
    13681 aggatatgtt cttagaagac tccttagacg tgctgctcgt catggtaagt tattaggagt
    13741 aaaagaatta tttttacaga aactaataga tgaagttata aaagtaaatg ataaagcata
    13801 tccagtttta gttgaaaaag aaagttatat taaaaaagta gttggaatag aagaagaaaa
    13861 attcaatgaa actatagacc aaggaacaga aatattgaac tcttatatag aagtattaaa
    13921 aaatgaagga aagactgtcc taagtggtca agaagctttt aaactatatg atacttatgg
    13981 cttccctata gacttaacta aagaaatatt agaagaagaa catctatctg tagatgaaga
    14041 agcttttaat gaagaaatgg aaaagcaaaa agaaagagct agaaatgcta gaggaaatat
    14101 ggatggagaa agttggaagg aagaccctct atcaaaatta gaatctacag ttgacagtac
    14161 atttaatggg tatagtgaaa tttatggtga aggaactatt gaagctatag ttaaagatga
    14221 tgagctagtt caaagtgcag aagaaggcga taaggtatct atagtactag ataacactac
    14281 tttctatcct gaaggtggag gacaagttgg agattgtgga ttgataacta atgaaaactt
    14341 agttttagaa gtattaaata ctaaaaaagg tgctaataat agtataaaac atataggtat
    14401 aataaaatca ggaagaataa gtaatgggga caaagtaaag acactcgtag atagagaaac
    14461 tagaatgtca gctgcaagaa atcacagtgc tacacattta cttcataagg cattaagaga
    14521 agtattagga gaacatgtta atcaagctgg ttctttagta actccagaaa gacttagatt
    14581 tgatattact cactttgaag cgatatcaaa tgaagaatta aaagtaatag aagaaaaagt
    14641 aaataatgtt attttatcat ctttagacat aaaatgtgat attatgaata taaaggaagc
    14701 taaagaaaaa ggagctacag cactatttgg tgaaaaatat ggtgatgagg taagagtagt
    14761 ttctatggga gattattcta cagaactttg tggaggaact cacttgacaa atacttctca
    14821 agtaggaatg ttcaaaatat tatctgaagg tggagtagca gcaggtgtaa gaagaataga
    14881 agcaataaca ggaaaagctg tttatgaata cctaaaagaa agagatggaa ttatctctga
    14941 agtttgtgta aacttaaaat ctaaagaaga taatttaatt caaagaataa gttctttatt
    15001 agaagaaaat aaaaacttat ctaaagaatt gcatgatatg aaagctaaga tgagtttaca
    15061 atcagttgat tctatttttg attctaaagt tgaagtaaat ggagttaatt tgataactaa
    15121 taaatttgaa ggtatggata tggatacact aagagaaaca gcagataatc taagagataa
    15181 gcttggttct ggtgtagtag tacttgcaaa tgtagtagat gataaagtta actttgtagt
    15241 tactgccact aaagatgtat tagataaggg aatacattca ggtaatattg ttagagaagt
    15301 tgcaaaaata gctggaggaa aaggtggagg aagacctaat atggcacaag caggagctag
    15361 tgatgtatct aaggttgacc aagctttaag ctatgcaagt gaggttatta agacacaagt
    15421 taaataacat tcactcaagg aggtttttag tttggaaaaa gatttagact acactatgaa
    15481 atttgaagga ataccagaag ataggatgag tgtaggagat actattgatt ttgtatataa
    15541 agcattagtt gaaaaaggat ataaccctat aaatcaaatt atagggtatc tcctttctgg
    15601 tgattctagc tatataacta gtcataaaaa tgcaagagct ataattaaaa aatttgaaag
    15661 agatgagata cttgaagaag taatcacgca ctatctgaat agaaagtagg tcaaataaat
    15721 gagaaaaaag attatgtcgt tgtttgcaat agtcattatg atttttgtat caactgctag
    15781 ctatggtttt gctgaagaaa aaggcaaggt catatttatt gatatgaata ggaccaatct
    15841 tggaagtatg atgaatattc caatcttgaa aaatgagata gaaaagagag gctatgtagc
    15901 tctcatgaat ataagagggg atcaaggtac tgatgataga aggtcttatg caagtatggg
    15961 tgcaggaaca agagctagtg taactactga agactatatc aattttcaaa aagtagacca
    16021 agatactgct aaagcttttg aagtcgcaac aggtcaaaaa gctaaagaaa taaatgattt
    16081 aggcattaat agatctataa atgaaaatct tgagtatgga gaatatggag caaatttagg
    16141 gtcattagga aaaactttat cagaaaatgg tttaaagaca tctgtaattg gaaatgctga
    16201 tataattgaa aataatcaat tagttaaaaa cagaaactta tgtttagtag ctatggacca
    16261 atatggaagg attgattcag gtaatattga taatattaat aagaaagatt ctaaaatgcc
    16321 atttggaata tctacagatt atgataaatt actagatgaa actaaaaaat cctataaaaa
    16381 tagtgatgct atatttgtag aacttggaga tacatataga ttagatatgt atagattaaa
    16441 tctaaatgat aatacttatg atacaatgaa gaaaaatatt gtaaaaaata taaataaata
    16501 tcttgaacaa gtattttcta tggttggaga aaatgacact gtttatatag caagtgcatt
    16561 tccaagtgac ctagattata agaataagag aagactttct ccaataataa aatttagtgg
    16621 agaagggaaa ggtctactgg aatctgccac aacaagaaag gctggaattg ttgccaattt
    16681 agatgttgga gtagatatac taggtacatt tggtcttgaa aataaagaga tggtaggtag
    16741 aagttatact ttagtaaata aagaggacaa tattagctat ctaaaatcac tttatgaaag
    16801 gatagtgtct atatcttcaa taaggtcaac tattataaat ggctttgtag gcgtaatatc
    16861 tgcttcttgg gtaattgcca tgtttgcgat tttctttaga aattatatgc cacataaaga
    16921 gcgtgtattc aaagtactta aggagtttat aaaattaggc ataataatgc cactatcatt
    16981 tatgttagct cctttgatga attttaaaac tccaatttca atatctttgg gaatatttgc
    17041 aactacgcta atattatact tgttaggtag aaaattattt aaggacaatg atataaagca
    17101 catgggattt tttgcaacac ttactatttt agtcatagtt atagattctg tatttggaac
    17161 atacttaatg caaaataaca taatgagtta tgatgctgtc ataggagcta ggtactatgg
    17221 tgtaggaaat gaatatgaag gtgtaactat agcaagtgca gtatttggct tagcagtatt
    17281 attacattat aagaaggtat ctaaattact tgttgtaatt ttttcattaa taatacttat
    17341 aactagtgca tatccttcaa tgggtgcaaa tgttggtgga gctatatcag aatgtgtagc
    17401 atatttgttg tttataatgc ttatatatga cattaaaata gattttaaga aagctgtatt
    17461 attagctgtt tcagctgtag ttgtcgtttt tgcatttgca gctttagata tactttcagg
    17521 tagtgaatct catttaggta tgtttgtaca gagaattttc ctaaatggac caggagagat
    17581 attacaaaca tttggaagaa aaattcaaat gaatatgcaa ctagcacaga caagtgtatg
    17641 ggtaaatatt ttacttgtag gaattggagt aattgcagtg cttatattta aaccatctag
    17701 acactttaga aagataatga ataattttcc aatcttgttt aaagggttta ttgcatctat
    17761 ggttggttgt ataattacac ttttagttaa tgattctgga atagttgctg catctacagc
    17821 atctatatat atacttatac cactaataat aataagtata aatatgataa tatttgaaaa
    17881 aaataactaa aaaaattttt actgttcata taggatttaa ataaaaaatg caatatgatt
    17941 attaatgtaa actggatgct tacataaaaa atcatattgc aatttttatg attgattatg
    18001 taaaattata tgaagcagta agcctgtgac cttagttatg agaggttcag aatatgtatt
    18061 taatctatag aatataaata gataaaggag gaactatgtt agatggaaga ataatgggtc
    18121 ttgatgttgg tgataaaact attggagttg ctgtgagtga tttgatggga ttaactgcac
    18181 aaggtgtaaa aacaataaaa agagttggca agaaaaaaga tattgaagag ctaaaagcaa
    18241 taataaaaga aaaacaagta aataaaattg tatcaggatt accaaaaaat atgaatggaa
    18301 ctttaggtcc acaaggagaa aaagtaatca agttttgtga attggtaaaa gcagaaacag
    18361 gtattgatgt agagttttgg gatgagaggc tttctacagt agcggcagag aggtctttat
    18421 tagaagcaga tgttagtaga caaaaaagaa aaaaggttat agatatgttg gcagcagtaa
    18481 taattttgca aggatattta gattttaaaa taaattcata aaagaactat aaaattgaat
    18541 acttatgata tataatataa ataataaaaa taaaaataaa tttgaggtga atgacattat
    18601 ggaagaaaat ataataaatt taatagacga aaatggcgtt gaaagccaat ttgaaataat
    18661 attaacatta gaagcagaag gaaaagagta tgcaatatta atgcctttag atgatgaaga
    18721 agctgaagaa gctttaatct ttagaattga tgaggatgaa gaaggtgaaa tcttaatacc
    18781 tttggaaagt gatgaagagt atgaaactgt tgtagctgtg tatactgcta taatggaaga
    18841 agaaggttta aattacgacg aagatgaatc aaatggactt aactagataa gtaattaagt
    18901 ttgtatataa aaatcagata tttattatat ttgctttaat taaaccttat taattgtaaa
    18961 agcatttata gtatatatct gtttttttgt tttttattaa aaataccaaa aaatttatga
    19021 attttggtga ataatatatc ttttatgggt atatatttat attatgagaa taaattaaat
    19081 ttatttaaat gtcttgacaa aaaatgaatt atcaattaca atattattta ataaagatta
    19141 tcaattgaaa atagtaaggg tgatgttatg gcaaatacaa tggatttatt aaaagacaag
    19201 ttaaaagaaa ctggttttaa gattacgcca caaagaagag caatagttga aatattgctt
    19261 aaacatgacc attcacatct aagtagtgag gaaatctatg atttagttag ggttgactgt
    19321 cctgaaatag gcttagctac tgtatataga acaatgcagc tattggatga aataggatta
    19381 atatcaaaac taaacttaga cgatggatgt ataagatatg agattagtct acataaagaa
    19441 gactgtcata atcatcatca tcttatttgt aaaaattgtg gaaagataat ggaagctaaa
    19501 gaagaccttt tagacaatat agaaaaagag atacaatcat tgtacaaatt taaaatactt
    19561 gatcatgatg ttaaatttta tggactatgt gacgagtgta atggagtaag cgattcagaa
    19621 gaataaactt ctattgctta atgtcgatgg aagaatagta tagaactttt atattctatg
    19681 ctattcttcc attttttatt ataaaccatc tataaaatgc aaatatacag ttttcaagat
    19741 gcttatagtt ttaatattga aaatataatt aatcctataa gtacaaaaat gaatgatttt
    19801 tctatgaaat tactaaatgg tcctccaaca gttaaaaaac ctaaagaaat tttcttttcg
    19861 cttagcggat aaaaaattgg tattccttgc tttgtaaata aatctcctaa aaacatatga
    19921 cttgcataac caatcgtacc ataaaaagat aaattattaa tattagtgat taattctatc
    19981 tgttttagta aaaaatatac tattatagtt gcaaatatgc tatgagaaaa acttctgtgc
    20041 tttaaccagg gaaaaattga caacattaga caaaaaattg taaaatatat ttcaacttct
    20101 atgatgtata agcatatagc taatagtata aatattaatg atagaaaaac ttttcttaga
    20161 aatgtgtggg ttagttttgc ttcaataatt ataattgcaa caaatgtaat tattgcgctt
    20221 aaataaaaat tatcatttat atttattgaa ataaagaaaa taataatatt aacagcatat
    20281 ataaaaattc tatatatgta tttggataca ttgtttttta gaatgaaatt tgagaccata
    20341 gaattagatt tatccaaatc gggtagtaca gcaaaagtag ctgaaactaa aatatcaact
    20401 aaagatatag gaattttaaa tattaaagat gtttggatag ctgttaagat tcctattgca
    20461 caatgagttt taccacgcat aaatcctcct aaattatccg atattaaatc atataatatt
    20521 tagaggatat caaaataaaa taggtttgtc aaaataatac gttaaatgta ttttaaatat
    20581 agcttaaaat atgatacgaa ttatggtaca ataagaataa ctggtagcga gtgcgtgatt
    20641 tgatattatt tatgattgta gaacgtgtta aaaaattaaa tagacagaag gagatgatgc
    20701 aattgttcaa aaagaacacc aacaagataa aggtaatggc cttgggtgga cttaacgaag
    20761 ttggaaaaaa tatgactgtt gttgagtata aagatgaaat aattgttata gatgctggac
    20821 ttagttttcc agaggatgaa atgttaggag tagatatagt tataccagat ataacttatt
    20881 tagtaaaaaa tagggataaa ataaagggta tatttattac acatggacat gaagaccata
    20941 taggagctct tccatatata ttaaagaaaa taaatgtacc tgtatatggg gcaagactta
    21001 gtataggtct tatacaagta aaacttaaag aacataaaat gaataatgtt aagttaaatg
    21061 ttataggtcc tagacaagtt ataaagcttg ataatatgga agtagagttt ttgaaaaata
    21121 accatagtat accagatgct tactctattg caatacacac agaccaaggt ataatatacc
    21181 acacaggaga ttttaagatt gacttgactc ctattgatgg agatgttatg gatatgcata
    21241 gaatctgtga gttgagcaaa aaaggagttc ttttaatgct ggcagatagt actaatgcag
    21301 aaaaaccagg atttactatg tctgaaaaga ctgttggggt aggtcttgac gaattatttg
    21361 caaaaggaaa tggcagaaga attatagttg ctacctttgc atccaacata cacagattac
    21421 aacaaataat aaatactgct gaaaagttta atagaaaagt tgctatatca ggtcgctcaa
    21481 tggtcaatgt agttggagtt gcaaaagaat taggatacct agatatttct gatgacatgc
    21541 ttatagattt aaatgatata tgtaagtatg aagatagtga attagtgata ataacaactg
    21601 gttcacaagg tgaacctatg tcagctcttg ctagaatggc tttttcagag cacaaaaaag
    21661 tagaaataaa gagtggcgat ttagttataa tatctgctca tccaatacca ggaaatgaaa
    21721 aattaatatc aagagtaatc aatttcttat ttgaaaaagg agcagaagta gtatacagtg
    21781 atatagctga tatacacgtt tctggacatg cctgtcaaga agaattaaaa cttatacatg
    21841 cattagttag accaaagttt tttatgccag ctcatggtga atatagaatg ttaaaaagac
    21901 atgctgaaat agctgaacag cttggtatgg ataaagaaaa tatttttgtc atgcaaacag
    21961 gagatgtttt agaattagat aaaaattctg ctaaggtagc aaaccgtata caaacaggaa
    22021 acatattagt tgatggtcta ggagtaggcg atgtaggtaa tatagtttta agggatagaa
    22081 agcatttatc agaagatggt cttatgatag ttgtagttac tatttccaag gatgagggta
    22141 aggtcttagc aggtccagat ataatttcca gaggattcgt atatgtaaga gagtcagaag
    22201 atttaatgga tggagcaaag gatataatta agaatgttct aaatgaatgt gaagagaaaa
    22261 atattaaaga atgggcttat ttaaagaata atattaaaga aaacctaaaa gaatatttat
    22321 atcaaaaaac taaacgaaat ccaatgatac ttcctatcat aatggaagta taatataaaa
    22381 aaactataaa tttgaaaatt agacctcttg taatttttaa agtaatatca aaaatccaat
    22441 tgatattatc ttaagaatta caagaggtct ttttaatact cattttaaaa aatatatata
    22501 ttacataaaa taccaattat aacaatcttt tattagtaaa aactacttat atataaattt
    22561 tactgatagg aggattctta tggatgatgt ttcaagacaa aatgctataa aagctttaaa
    22621 acaaacaaaa atggaaatag caggagagta tggaatgaat tatgaagatg cttttgaaat
    22681 aatagaaaat gcgtctaata aaggtgtttt agaaggttac tttaaaaagc ttgaaaagaa
    22741 aaaaaattta ggtcaaggta tatctaggca tttagaataa aaaaagttaa atgtgagaaa
    22801 ataagtttaa ataaacttta agaggtgaca tcgatgaaaa aattagctag tactgctttg
    22861 gctatattaa ttgcgcttac tcctttaagc ttttcttttg caaataataa ggaaaatgct
    22921 gatgccaacc aactaaatat atcttcaaaa tcagcaattt tgatggatgt tggtagtgga
    22981 caaattttat atgaaaagga tgctcataaa aagcttcctc ctgctagtgt aactaaggta
    23041 atgactatgc tacttatagt agaagctttg gattcaggca agattaagct tgatgatgaa
    23101 gttcaagtaa gtgaaactgc atctagtatg ggtggtagtc aaatattcct agagccagga
    23161 gaaactcaaa aggttgatac attattaaaa ggtatagctg ttgcttctgc aaatgatgct
    23221 tgtgttgcta tggctgaaca cttagctgga agtgttgaag gatttgttga tagaatgaat
    23281 gctaaagcaa aagagttaaa tatgaatgat actcattttg caaatacaaa tggacttcca
    23341 gttgcaaacc actatacatc tgctcatgat atagctttaa tgtctagaga actattaaaa
    23401 cacgaaatga taagtaaata cttaactaca tggatggata aggttgtagt agggaagaaa
    23461 caagttacag taggtcttgc aaatactaat aaacttataa agcattatca aggagcaact
    23521 ggtgttaaaa ctggttttac tcaagaagca aaatactgtt tatcagcatc tgcaaagaga
    23581 ggtaatactc atctagttgc tgcaacttta ggagcagaga cttctccaga aagatttaat
    23641 gatgcctcta gccttttaaa ctatggattt gctaattatg aaagtgttaa gttatgtagt
    23701 aaaggtgata atatagcaac acttacattg gataaggctg atgaaaacaa agttaaattg
    23761 gttgctaaag aagatttaaa tgctcttatt aaaaaaggaa gttctaaaga atttgagaag
    23821 aaaattgaaa tagtaaaaaa tcctaagatg ccaattaaaa agggaactgt tttaggaaaa
    23881 ataaagatat gtaaagataa aaaagtaata ggcgaagtgg aattgataaa tactaaggat
    23941 ataaataagg caagctatct acaaatgctt caaagaataa ttgataatat gatataaata
    24001 attatcagat gaaatataat tataatacac aaagatatga ttataataca cataaaaaat
    24061 acccttatgt aattatttgc cataggggta tttttattta ccatatagaa gtattttata
    24121 atcttatgat ttttatagaa atatagaaaa gtttatgtat cttaaaatat catttgattt
    24181 aatatcatat atagggttgt ttaacaatta tctaagtaaa ggcatactac cagtaagttt
    24241 attcactttc tttttattcc cataaattgc tatcccaaaa tactgtaact catcttctgg
    24301 aacactagca atcttttgtg taaattcttc ataaagattg caactttgtg ctacatctga
    24361 aaaatctaca acagttaaat cgttaaattc caaagtatat aattttttac gtaaatcttt
    24421 tattatttct ttgtttcctt ttaaaatagg tacaggaatg tcaataattc ctaagtggct
    24481 attacctgtt ttgtcaatta catctgggcc aacagtttct ggggcatgtt ttcctagtgt
    24541 aattcccatt atagctgctg tattggatat aattcccatt ggtaaatttt cgtctattac
    24601 catgacacat tttgaatttt tattttccat ttttatttct cctttttacc ttatttaaag
    24661 ttgtttgaat catcatttat aaatatattt tgatactgct ttggtgtaag accaataaaa
    24721 ttcttaaaaa agtttgtaaa gtgactttgg tctacaaatc ctgtttgtat cgcagcatct
    24781 ataggttcta cgcctttttc taaaagcttt ttagctttgt ttacccgtat attttctagg
    24841 tatctatatg gtgtaatacc ttttaatttc gtaaaattac gaagtagaga gtacttattc
    24901 atacctgcta tagtgcttaa ttcgtcaagt actatatgtt cagcataatt attttctaga
    24961 taatcacata ctgcttgaat ttctatattt gtattttcca agttagattg caaatttggc
    25021 tctgtatgtt cttcaattaa ttgttcaatt aggaagaaaa aaagctcttc ctttttaaaa
    25081 tccaattctt cttccatgat tatatagtgt aattcttgta gtagaggtac tagctcactg
    25141 cgaaatataa caggttgatt aaattttggt aaataatttt tacctgtaat ttcaaagacg
    25201 gtttttttca ttatttctgg cttaatatta atacatctgt aatcaagtac tttatcatct
    25261 atttgttcac aagtatgact atcaaaaggg ttaaaaagta gcaaatctcc agtacttgtg
    25321 gtatactctt tatttttaca agataaatat ctttgacctt tttctataaa accaattaca
    25381 taatgttcat gaaaatggtt tgggaatttt tgcattatgc ctttaaatct atatgcttca
    25441 atttttaatt ccctatcaaa gcatactgtg cgttcttctt gtctcactta ggtaacctcc
    25501 tttcatgtta tacaaagtgt aacacatatt tttttatact tcttgtataa tattgactaa
    25561 gagacaataa tgtcaattca tgtcaatata atattacact tacagtaggt gtaattatta
    25621 tattttcaca atgaatatga tataataata aaagttataa aataaaaagc aaattttaat
    25681 tttatgccta gaagttaaaa ttattataat gtaaaataat tttgattata agctatttaa
    25741 ttgttataat aatattaaaa atttaattta ggagatgtac attaatgtta agtatgaata
    25801 taggagaaat agtagcaagt ttagttggta tagctatggc aatatcaata catgagtttg
    25861 ggcatgctta ttcagctcac ttattgggag atgatacagc taaagcttat ggtagaatga
    25921 cattaaatcc tgtaagacat gttgacccaa taggtctttt ggctatgttt atccttaaga
    25981 tagggtgggc aaagccagta ccagtaaatc caaataactt taaaaactat aagataggaa
    26041 acctgatagt ttcacttgca ggcgtttttt gtaatgtttt gactgctata ctgtgtgtaa
    26101 ttatcaataa gtatgtgcat atgtatgcta taaatacaat agctggatat gtgcttttat
    26161 atagtatggg atttgcagct tttaacttac ttccgatacc tccacttgat ggatggggag
    26221 ttatatctac atttgtacca tataaatgga atgaatattt gtataaattt gaaagtatga
    26281 gttatataat tttattaatt gctttattta ctggtatgta tagaataata ttaacaccaa
    26341 tatatgcagt gttgctaaaa atagtatatt tatttgttta atataataaa cagattaaag
    26401 agggattgat atgaagtata atatacaatt acaagtctat gagggaccat tagatttact
    26461 ttatgattta ataacaaagc ataaaataga tataaaggat atatccataa tagatataac
    26521 taagcagtat ctaaattatt tgaaaatgct agataaaatg gatttagaga taactagtga
    26581 atttataact atggcatcaa aacttttaga gataaagtct aaatatctgc tttataagca
    26641 aaaagatgaa gaagaagacc caagaataga acttatggaa aagttagaag aatatagaaa
    26701 gtttaaggta gcatcacaag atataaaaga aaatataact tatgttaatg agagatttta
    26761 tagaaataaa gaagagataa ttattgatga caatgtggat ttagaagata tatcaattga
    26821 agcaataaaa aatatacttc catacatatt taaagttaaa acatcacaga tagaaaatgc
    26881 taatgatgag aaactagata aaatagtaag gaagaaaata atatctgtag aagaaaaaat
    26941 attatatata agagatatta taaaagagaa gattgaagtt acttttacaa atattataaa
    27001 atcatatgaa aatgatgaaa taatagctac ttttttatct atattagagc ttataaaaga
    27061 aaaagaaata gtcgtagtac aagatatatt ttttgatgat attctaataa gaaaaagttc
    27121 ggagtgttaa aatgaaaaga gaagatataa agtatattat agaatctgtt atgtttgcat
    27181 atggtgagcc aataagtata aaagaattaa actatataat aaataaagaa ctttcatcaa
    27241 aagaaataga gattatgtta aatttactta ttgaggagta cagagaacaa aatagaggta
    27301 ttcaaataat caagttagag aataagtacc aaatgtgtac aaataaagac tatgcagaat
    27361 atataaagaa gattattgaa ccaaagaaaa agaaaagttt gagccaagct acattagaaa
    27421 ctcttactat aattgcctac aaacaaccaa taacaaaggt agaaatagag gatataagag
    27481 gagtaaaatg tgataaggta ttacaaactt tgtttgaaaa tgaacttata agagaagctg
    27541 gtagattgaa taagataggg aaaccaataa tttataaaac aacagatgaa tttttaaagt
    27601 tgttgaatat agaaagtctt gaagaattgc cacctataga aaattatcaa gaagtggcaa
    27661 caaatgaata aaaatataaa atcacaataa tttagtatgt aattttaaat tttaaaagat
    27721 ttaaaatgct ctaagttgtt gtgttttttt gtataaatat aaaattataa ttctctt
//
LOCUS       DAEMIK010000040        27403 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEMIK010000040 DAEMIK010000000
VERSION     DAEMIK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.040"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1859)
                     /locus_tag="KPK87_001467"
     CDS             complement(<1..1859)
                     /locus_tag="KPK87_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537625.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIF"
     gene            complement(2042..3517)
                     /gene="spoIVA"
                     /locus_tag="KPK87_001468"
     CDS             complement(2042..3517)
                     /gene="spoIVA"
                     /locus_tag="KPK87_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG2537626.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3689..4984)
                     /locus_tag="KPK87_001469"
     CDS             complement(3689..4984)
                     /locus_tag="KPK87_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2537627.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSVINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPIASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMVYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENATKVS"
     gene            complement(5095..6246)
                     /locus_tag="KPK87_001470"
                     /pseudo
     CDS             complement(5095..6246)
                     /locus_tag="KPK87_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986090.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(6298..7437)
                     /locus_tag="KPK87_001471"
     CDS             complement(6298..7437)
                     /locus_tag="KPK87_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2537628.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDTKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            7756..8406
                     /locus_tag="KPK87_001472"
     CDS             7756..8406
                     /locus_tag="KPK87_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2537629.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRNVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(8563..9582)
                     /locus_tag="KPK87_001473"
     CDS             complement(8563..9582)
                     /locus_tag="KPK87_001473"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG2537630.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(9613..10227)
                     /gene="plsY"
                     /locus_tag="KPK87_001474"
     CDS             complement(9613..10227)
                     /gene="plsY"
                     /locus_tag="KPK87_001474"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG2537631.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10262..11581)
                     /gene="der"
                     /locus_tag="KPK87_001475"
     CDS             complement(10262..11581)
                     /gene="der"
                     /locus_tag="KPK87_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG2537632.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTYFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(11615..12943)
                     /locus_tag="KPK87_001476"
     CDS             complement(11615..12943)
                     /locus_tag="KPK87_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG2537633.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13181..13381)
                     /locus_tag="KPK87_001477"
     CDS             complement(13181..13381)
                     /locus_tag="KPK87_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537634.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13383..14315)
                     /locus_tag="KPK87_001478"
     CDS             complement(13383..14315)
                     /locus_tag="KPK87_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG2537635.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14360..15292)
                     /locus_tag="KPK87_001479"
     CDS             complement(14360..15292)
                     /locus_tag="KPK87_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG2537636.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15347..16636)
                     /locus_tag="KPK87_001480"
     CDS             complement(15347..16636)
                     /locus_tag="KPK87_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2537637.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDMVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(16665..17357)
                     /locus_tag="KPK87_001481"
     CDS             complement(16665..17357)
                     /locus_tag="KPK87_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537638.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17372..18133)
                     /gene="pgeF"
                     /locus_tag="KPK87_001482"
     CDS             complement(17372..18133)
                     /gene="pgeF"
                     /locus_tag="KPK87_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG2537639.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18130..18591)
                     /gene="nrdR"
                     /locus_tag="KPK87_001483"
     CDS             complement(18130..18591)
                     /gene="nrdR"
                     /locus_tag="KPK87_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG2537640.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(18613..18876)
                     /locus_tag="KPK87_001484"
     CDS             complement(18613..18876)
                     /locus_tag="KPK87_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG2537641.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(18980..19753)
                     /gene="sigG"
                     /locus_tag="KPK87_001485"
     CDS             complement(18980..19753)
                     /gene="sigG"
                     /locus_tag="KPK87_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG2537642.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(19845..20567)
                     /gene="sigE"
                     /locus_tag="KPK87_001486"
     CDS             complement(19845..20567)
                     /gene="sigE"
                     /locus_tag="KPK87_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG2537643.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(20592..21464)
                     /locus_tag="KPK87_001487"
     CDS             complement(20592..21464)
                     /locus_tag="KPK87_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG2537644.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(21754..23028)
                     /locus_tag="KPK87_001488"
     CDS             complement(21754..23028)
                     /locus_tag="KPK87_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2537645.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23312..24469)
                     /gene="ftsZ"
                     /locus_tag="KPK87_001489"
     CDS             complement(23312..24469)
                     /gene="ftsZ"
                     /locus_tag="KPK87_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG2537646.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(24659..25012)
                     /locus_tag="KPK87_001490"
     CDS             complement(24659..25012)
                     /locus_tag="KPK87_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG2537647.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(25111..25785)
                     /locus_tag="KPK87_001491"
     CDS             complement(25111..25785)
                     /locus_tag="KPK87_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2537648.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(25790..26488)
                     /locus_tag="KPK87_001492"
     CDS             complement(25790..26488)
                     /locus_tag="KPK87_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2537649.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(26511..27251)
                     /locus_tag="KPK87_001493"
     CDS             complement(26511..27251)
                     /locus_tag="KPK87_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG2537650.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
BASE COUNT         8486 a         5054 c         2820 g        11043 t
ORIGIN      
        1 aaaaaaataa tatctttttt tagattatat tctttagatt ctaatttata attattattt
       61 attattccat cataatcact tatatttgac ttatttttat ttgtatcttg atttttgtta
      121 gaaatacttt ccctataagg aaaagaatgt tcattggtac caaatactga ttttgagctt
      181 aaatcataat tatcatcatt ttctgtaatt gaatttttat tgtaaaatcc tttttcttta
      241 tcagaattat tttctttatt accgtcttta ggtatatcat gaggattttt attttcattg
      301 ctatcagtac ttgattctac atcatcttta ggagtatttg gttgtttctc atcagaatta
      361 tcattttcat tattattatt ttcattatta ttgttttgat tgttattact atcattattt
      421 tcatttccat tattcccatc ttgattgtta tcatcctcat ctctatccgg gtcgtctcta
      481 cctcctcctc cacggtcacc atcaatatca tcattttccc gttcatcatc tggtgtctct
      541 tggttgtctc cgcctcccat tataaatcct ggtttaaaaa ctatatttga atacccttct
      601 tttaatcctc ctaccacttt tactctaaaa caacgcaact taaaatcttc aaaaaaaagt
      661 atcgcttgat ctctaatatc ctcctgttct tcagatatcc aattgattcc atcgcttgaa
      721 tattcaacaa aaaccttact tgcactatac ggcatatcat aaaataaaac agctacatat
      781 ccattttttg tattttgaaa ttccagttcc aaattgtcta ttggtttttt ctctataaca
      841 gatacattca aaataggaac tacatcatca tttattataa ttttttcttt atttaacatt
      901 tctgttttaa actttcctgt tatattaaaa tcttctccct tttccaactt ttcataataa
      961 tcagtaaaat cccattctac atctatatca agtcgattac cactagatgt ttctaatacc
     1021 atatatttgg gaaattttat attttctatt ttatcatctt tacttacttt tatttcatca
     1081 ctaaatacac caattcccct atgacaacat ttcacaatat tatagccaga atcttcagct
     1141 atagagggta taagctcatt ataatcatca attatataaa ctttcttact ttctgatata
     1201 attgattgtg ccaacttatt attatctctt agtaacccac tttcattgtt gctactatat
     1261 gcttttattg aagtttcttc tatctcaaca tcacctttag aataaacacc tatagcatgt
     1321 tctccattca cttcaatatc cttatttagt attctaatat cattttcagt ataaattcct
     1381 attgcatctt ttccatttgc ctttattttt tcaacagatt cgtaggatga tttaatatgt
     1441 aagtctcctc cttcttcaac gtataaggct gtcccatcag tagcaattat gttgtttata
     1501 ccaatactta tacttccacc attttctata tgtattactc cttcttttcc tccttcacct
     1561 attatattca attcattatc atcaataata aatctcccat gattttttat caatatttta
     1621 tatttgttgg tatctattgt aaaattactg tcataaggaa tataaaatct aaactcttca
     1681 tctattacca tatcacaaga aagagtataa actatttctt ctttattatt tttattttct
     1741 tgaacccacc tttcaaaatc ttcaagacaa ttttcctctt gtccaaaaac tttatgtgga
     1801 aaacttataa aacacaaaac tattatactc ataaaaataa atatctcata ctttttcatc
     1861 ttacactcct agaaattttt agctttgaaa caatcctgtc gaaacatata cttcacccac
     1921 tataattttt ccacaattat caattattgt caatttatat aagtatcttt attaattgtt
     1981 ataaataaaa agctacttac taaagcaaac agacgtttca ctttaataag tagctatatt
     2041 tttataacaa aatagttata atattagaac ttccttcatt tacaattttt tgtaaagtct
     2101 tttgcatttt aactcttatc tcttctggca tagtatataa tttactttgt aattgctctt
     2161 ttactaagtc atgtagtgat ttaccaaaca tatttgattc ccataaatct gctggttgct
     2221 cttcaaatac ttccataaga tattttatca tctcttcacc ttgattttga tttccaacta
     2281 ttggagaaac ctctgtagat atatctgctt ttataatatg aagtgatggt gcattggctt
     2341 ttaacttgat accatattgt tttccttgtt tcattatttc tggttcttcc agactaagtt
     2401 cttctaagga aggagctact actccatatc cttttatctt ggcatctatt aaagcacttt
     2461 ctattttatc atactcattt ttaactttag atagtctagt tatcagacta agcagttgat
     2521 aatctccttc aattttaaat ccactttttt cttcaaggac attatagaat aaatcttgct
     2581 ttgtctgtaa atcaatattt ataacacctt ctcctagctc tacattatca acacctgtat
     2641 cttccaaaaa ctctaactca gaaaatcctt gtataatacc ttctatatcc cttattttac
     2701 ctatatctat aatactttgt tttaatgttg ttattatgct actttttatc caatgatttc
     2761 tttcaagacc ttcaacccat tttggtaagt tgattcttat ttctgtaagt ggaaaatcat
     2821 ataatacagc ttccataacc tcctctatat cttcttcttc catttcaacc acattcattg
     2881 gaagaactgg aacttcatac ttttcttcca attcacttct aagcatactc gtttcctcac
     2941 tttttggact taacgtattt aaaaccactg caaatggttt tttaagattt ttcaattctt
     3001 gtataactct ctcttctggt tctacatagc tttttctatc aattccagta actgaaccat
     3061 ctgtcaagac aactatccct attgtagaat ggtcttttat aactttttta gttcctatct
     3121 ctgctgcctt ttcaaatgtc atagcttctt gtgaccaagg agtggatact aatctttgtt
     3181 ttccaccttc ctcatgccct aaagctcctt caactatgta tccaacacaa tctaccattc
     3241 ttacttttaa agatactgtg tcttttattt ttatttctac accatctgct ggtacaaatt
     3301 ttggttctac tgtcattatc gttttacctg aaccactttg gggtatctca tctcttgttc
     3361 tatccttttt aaattcattg tctatattag gtataaccaa cttttccata aattttctta
     3421 taaaagttga ttttcctgtt cttacaggtc caactactcc aatatatata tccccctgag
     3481 tccttttgga tatatcttcg tatatgttat tattcatatt attcctccta tacatctaat
     3541 cttattaaat tatatttaga gttaagggtt aatattctta aaatagaaaa ataaagacca
     3601 gcaatattaa tttaatttac atatagaaat ttgtaaattt caatgtaaat taaattaata
     3661 ttgctggtcc aaatagtagt gtattatatt aactgacttt agtagcattt tcatttgaat
     3721 tattattcat ctcaataagt tctttacgtc tttcttttgt catgttcatc catataaatg
     3781 taaacaccaa tccaattatg gctactatag taagaactat gaagtagtac ctatatccag
     3841 ttacacccgg aaatgcatcc aatatcttcc ctgctgctaa aggacaaagt acttctggaa
     3901 gatatcctaa cgtagcaact attcctatag cagttcctga tattgataat gaataattac
     3961 cttcttcaag taacgaatag tgtaaacctt gaattgcata catagataca tatattgcaa
     4021 cacatccaat caatagtata taaaccatag atgattttgt tggtgtaaat attacaccta
     4081 aaagacctgc aatcataagt ataaatccat aagatataat ctttgatgaa ccaactttat
     4141 ctccaagtaa tccagaagca gctgatgcta ttggtctaca atactgagct aatattgaga
     4201 ttgcagatgc aaacaccaca gtagctccaa atgcttcagt tgaataaggc gtaaaataat
     4261 aaaatgacat attagctgaa tatgaacaaa acattattat tgttattatc catgtatgag
     4321 gcatttttat tactgcaaaa aaggcttctt tatcaaattt tgctgatacc tctttttcat
     4381 tatcctttaa tttgaaaaat actaatactc caactattac atttattact gagtataata
     4441 ctataatagc tgtaagacca gatttatcac ttacttttga tgaaatatac ccaaatagta
     4501 tcaatactag agatagatga attgcattgg taattccacg accactttcc ataaatccaa
     4561 aagcttttcc ttgttcattt tcagatgcta acattctaac agcttttaca agagctggcc
     4621 aaaaagtaag tattgttgtt attccccata aggcatggat taaaaacaca acaatatatg
     4681 gaggataaag tgttaataca agaccagata ttcctgtaat aattaaagaa cctgaaagca
     4741 attttctggc tgaaatacgg tctgctaaaa aaccaccaaa aaggtatgat accattgcaa
     4801 gtagaccata aatgcttcct aagcttccca tttgtgtatt tgtaagatta aatgcttgtg
     4861 caaaagtatc ataataataa ttctttaaat acggtaaagc atatataaat gctcctgcta
     4921 caaagagtat tatcaagact ttgaaatttt tccaaatgct tggtttattt acttcaactg
     4981 acatacttcc tccttatgta aatacctgta aattttcatg ataattaatt attgcttatt
     5041 gtattgttta ttcaatattt cggaatattt attaatatat taggttaatc tatctctaat
     5101 ataagatgta aaaagcaaga catatggaaa taatgctgtt aatgctgtca tagtaacaag
     5161 aacattgtat atattatcta ctgatggtaa taagtttgtt cctacaagaa gtagactcac
     5221 tattatagcc tgaactatta ctgcatttga tggtatattg tatttatttg taacagtcaa
     5281 ttgcttagga aatacacctt ctcttacatt tccaaaaagc atctttactg gacctgctat
     5341 ataaagtatt aatgctccta gtactgataa agttattcca aatgctacta ttcttataaa
     5401 ccaacttcct atacctaaat cttgtgcaac tttagctaat gcatctagta tccctgtaga
     5461 tgctgcaatt ttatctggag gaagtatcat tgttattgct actgaaccta agatatagat
     5521 tcctcctatt aaaacagcca cagttaatat agctttagga aagtttttct tagcattatc
     5581 catttcggtt atgaaattag cagtcgtttc agctcctgat aatgcaaata ttatagcaga
     5641 tatagaaact aatgaattgg catctatttt tggtattata ttttggattg tatatatcga
     5701 tgctgatgga gcttttttga gaataactac agacataaat gccatcacta taagtaatat
     5761 agcaggaatt gtagaaccta atgctcctgt attcgtaaaa aattttccaa atgccatccc
     5821 acgagtactt actaaggaca acagccaaaa tattatgagt gaaagtgcta aaataaacat
     5881 cttattgttt gataaatctg gatttccaag catatatgca atatttatcg ctaaaaaagt
     5941 caagaaagaa gaataccaaa atatctttga tgtccaattt agccatgaca ccataaatcc
     6001 ccacttctct ccataagctt gtttaaccca ttctccaagt ccaccatttt ttggatatgt
     6061 tgcagccaac tctgcacata taagtgatgc aggaacaaag aatataaaag caaacaataa
     6121 ccaaactggt atagaaccaa gacccaatcc aaaactagag gcagtagatt tagctatcca
     6181 tctaatccca aatagtgctg atatattcat aaatactaaa tcccaaaatc caattttctt
     6241 tttcactgta attcctccaa tttcaaattt attcagactc atacacaatg tattgactta
     6301 aatatcaagt ataagttctt caacacttct tggataatta gttataattg tagcaccatt
     6361 cttacctact attgctgtat ctgagtgtct aaatcctcca actccaggta tatatatccc
     6421 tggctccata gaaaatacca ttccttcttc taatattaat tcattgtcaa atcttaaata
     6481 tggctcttca tgttctgata aacctaaacc atgaccaatt ctatgatttg aatataactc
     6541 tagaccatac tttgcaacaa cagctcttgc tgcttcatcc accattcttg ctggtatacc
     6601 agcttttatt gtatctaatc cagcttgttg agcctctact gcaatcttaa acacttcttt
     6661 ttgtctttct gttggtttgc ctataataaa agttctttca ttttctgctc tatagttttc
     6721 ataccaaacc tgtctactat gtattactat atctcctctt tgaagtattc tagtattgga
     6781 atctaaatga ggttgtgctg ttctatcaat ccctgaacaa gtccagtttg caaatcctat
     6841 atatgtatca ggataatttt cagaaacata ttttagtaaa gcattatccc ctgcaacgtc
     6901 aaactccaat tcactcatcc caactctaac attttctaaa gaccctttta ttcctatatc
     6961 agataggtaa cctgctatct tcaaaaattc tatttctttt gtatctttta catatctcat
     7021 ttcaaatatt ttacttccaa catctttgat agcaaatgac ttatcagtta tatactctga
     7081 aaagcttgca gatactatat ctttttctat tccaacagtt gttcctttag ggtatttatt
     7141 taaaattata tctaagtaat gtttatgtga tattccatgt tctttcattt ctggtatttc
     7201 ataatacaca tatacattat caacttttgc aacttcttta gcatgtaatt cttctaatcc
     7261 aggtactatt aattctattt tatcttgagt tactactgtt acaattggtc tagaataagt
     7321 tattgcttta aatccactaa tataaaattg attgtctggt tcaaatatta aagcaatttc
     7381 aatgcttttt tcattcatgt actttctcaa tctttctagt ctcatattat ttgacataat
     7441 gcacctcttt cttaatattc agataattta ttcaatacaa caaggatgaa tatattattt
     7501 taaaattctt aatattttta gtacttgtcg tatttgtatg taaattaatt ttatttgtga
     7561 ttattttatc ataatcatga ttattttgtc aatcatgatt ttattttaaa aaatttttta
     7621 tattctagtg attttaaatt atctaaaata taacgagtat acattgtatt ttgtaatatt
     7681 attcttgatt tatggtaagt ttaatgataa aatcacgatt atataaacta tattgattat
     7741 ttgtaggtga ataaaatgac aaataaagaa ttacaaaaga aaaggatatt gatgtatttc
     7801 atagaagctg ctcaaaatat tatggaaaac gaaggtatag aaaatattac acttcgtaat
     7861 gttgcagata tggctggcta taatagttca actctatata aatactttaa aaatttagac
     7921 catttaatat catttgcatc tataaaatat tttaaagatt ataacttgaa tatttcaaga
     7981 tgtatagaaa atgtcaatga tgaatataag aaatatataa ttatgtggaa attattttgt
     8041 aagcattctt ttgataatgc tcaagctttt taccaggtat tttttaactt gtctagtgat
     8101 gaacttagct atataacaaa acaatattat gatatgtttc cagaagattt aggtattcat
     8161 gatagtgaca tatctttaat gattacagga gaaagtataa aggaaagaaa taagatacta
     8221 ttaaacaatc ttgtagatac tggatatata ccagctaaag atttagacat aatcaatgat
     8281 ataattattt ctttttatca aaatctatta ttcttaaaaa agaacacagg aaaaaattct
     8341 gatgatgatg aattaactat gaaaatgatt tcttctattg aatacataat aaaacatgct
     8401 aaataaaatt aaaatatggc tgtctcaaaa tagagataag ccttttttat tgttgaaata
     8461 ataaaaatat atttaatttc atataaaaaa gagtgcctcg taaactaaaa agttcatttt
     8521 gagacacccc taaaataaat ttattattta ttatacaata gtttatatat catcacctaa
     8581 aactacttct tccatttcat gctttttact tctcatcatt agttctattc caacttcttt
     8641 aggatttgaa cctttcttta ttacagaata tatagcatta gttataggca tatctatatc
     8701 taatttttca gcaacatcat gtgctacttc agtagcagtt atgccttcta caaccatctt
     8761 tacttctttt aaagtatctt ctaaactcat ccctttacct atgagtatac cagctcttct
     8821 atttctacta tgcatacttg tacaagtaac tattaaatct ccaatacctg aaagacctgc
     8881 aaaggtagac atattagctc ccatagcaat tccaagtctg ctcatttcac taatacctct
     8941 tgtcataagg gcagcttttg cattatcacc ataccctaat ccgtcacata tcccagctcc
     9001 aaatgctata atatttttta atgctcctcc aagttctact ccaactatgt ctggattcgt
     9061 ataaactcta agtttagggc tcataaataa atcttgtatc atctgtgcta tttttaaatc
     9121 tttagaagca actactacgg tagtaggaat atcccttgct acttcttcag catgagatgg
     9181 cccagatagt attacatatg gattttgtgg taattcttct tcacagactt gagataatct
     9241 aagcccagtc cctttttcaa gacctttagc tacattaaca ataacttgtc cttcttttat
     9301 aaatggtttt attttttggc aagtacttct tacagcctgt gatggaactg ctaaaactat
     9361 taccttacaa tcttttatag ttttttctat atcagtactt atagttatat tatttggaaa
     9421 taatactcct ggtaaatagt ctgtattttc tttagttctg tttatttttt tagcttgttc
     9481 ctcttttcga gtccacatgc tcacatcatt accttttttt gcaagcccta aagctaatgc
     9541 acttccccag cttccagtac ctaatacaca tactttctcc atgtttaaca ccacctgagt
     9601 ataatatatt tactattctt tcttttgacc aatttttctc tctgtcccat ttaataatcg
     9661 ctttatattt gctctgtgat tatatattac tgatgcagtt aagaacagag ctactattaa
     9721 accagcttta ttttttacca taatcataaa tattggtgaa catcctattc ctacaacaga
     9781 acccaatgaa acatactttg ttatagctac caccaatata aatactgcta gacacattag
     9841 tgttattact ggatttacag ctagcattgc ccctagtgaa gttgcaacac ctttgcctcc
     9901 tctaaatcct aaaaccgctg gccaattatg acctgctaca acacatataa ctgccagata
     9961 tccagctaaa agagtatcta ttccaaccaa tcttgctgca aattctgata ttaaaacagc
    10021 cattacacct ttaagaacat cacctaaaaa tgtcattgct cctgctcttt ttccaagtgt
    10081 cctaagtaca tttgtcgaac ctgcattacc agaaccttga gtccttatat caaccccagc
    10141 aattcgcttt gcaactatgt atgaggtaga tatatttcct aaaagatatg caactacagc
    10201 aataattata taactaaata tctccactga atttcccccc tttagttata ttttaagata
    10261 tttatttttt atttttttgt ctatactcaa actttatgga agttccttca aatccaaaat
    10321 tttctcttat cttattctca aggtatcttt gatatgagaa gtaagttaaa tccttatcat
    10381 ttataaacaa tgtaatttta ggaggtctaa tgtcagtttg agtcccatag taaattttta
    10441 atcttctacc tttgtcagat ggtggttgat ttaacattac agcttctcca attacttcat
    10501 tcagtgctga agttgatatt ctcttagaat gttcatttga cacatattca actgtatcta
    10561 gtatcttgtt cattctttga ttagtttttg cagaaacaaa tacgattgga gcatacatca
    10621 taaatggaaa tttttctttt atatcttttg tgtaattact catagtctta ttatctttct
    10681 ctattaaatc ccatttattg attacaaata tgcatccttt tccttcatca tgtgctatac
    10741 ctgcaacctt tgtatcttgt tctgttacac cttctaaggc atctattact atcaatacta
    10801 catctgctct atctacagca ctcatagctc ttattacact gtatttttct attgtctcat
    10861 atattttact ttttcttcta agccctgctg tatctattag aagaaatttc tgtccatttt
    10921 tttcaaaata agtatcaatt gcgtctctag tagttcctgc aattggactt acaattactc
    10981 tttcttctcc aagtatctta ttgagtatag aactttttcc tgcatttggt ttacctgtta
    11041 tagctactct tattatatct tcttcatact ctgtatctaa tcctgctgga aaattttcaa
    11101 ctatctcatc taataaatct ccaaatccca tactattggc tcctgaaact gcaaaaggcg
    11161 ttccaaatcc caactcataa aaatcgtata tattatcaaa ttgactttga ctatcaatct
    11221 tattaaccac taagattact ggtttttttg tctttctaag caattgtgca atttctttat
    11281 cagcagctgt tattccagct ttaccatcaa ctataaaaag tattacatgt gacatgtcca
    11341 ttgcaagcat ggcttgattt ctcatttgag ataatattat atcttcgcta tcaggttcaa
    11401 ttcctcctgt atccactaat gtaaaatatt tatctaacca ttctacttca gcaaatattc
    11461 tatctcttgt aactccaggt gtattttcaa ctattgatat cctttttcct gcaaatttat
    11521 taaatattgt agacttacca acgtttggtc tcccaactac agctacaact ggcctactta
    11581 tactcatttt atttctcctt ccatcttttg ttttctattt taaaatttta tccacaaaat
    11641 cttttccttc attcaaacaa gggataattt ttattctcat aagttcttct aattgattta
    11701 attcaatatt atctaagaaa atttcttcat ctgcttttag catacttctt gttatataaa
    11761 gagcttctcc taattcttta tcttcaagtt gttcttttaa atccttagct gttaaaagac
    11821 ctgatacagt tattgtctct ccaaaaaact tgtttttgat ttcatataca tttacttgaa
    11881 gatttttaaa cttgtccatc atagcatctg ccatactctg tataaattca tatgcagaat
    11941 gaccagtagc tattgatact ttcttctctc tttttagaat cttctcaggc aatatattta
    12001 gatactcttc aatctctgtt tttaacttac gtatcatacc tacaccatct tcaaactgta
    12061 aaaaaccttc atattcttca tatccaggca attcactatt agatagtata taaaattcat
    12121 ctgataaaaa tgcaaatcga gtacctaatt tttcaagata tttctgttgt atttgatgta
    12181 tttgctctat agttttactt gcgctcttat cattaaatat atttaattct actagatttt
    12241 ctctatgctt tgtaatccca actggaacta tcgcaacact gtttacatac ggataaagtt
    12301 gagctagttc ttttattgtt ctatccaatt cctttccatc atttatacct ggacataaaa
    12361 caatttgaca gttcatttct atatgagcat ctgccaatct cttcattata ccatataatt
    12421 ttcctgcaaa tttattgctt atcatttttt gtcttaactc tggattagtt gtatgcacag
    12481 atatatttat aggacttatt ctatatttta taatattatt tatatcttct tcactcatat
    12541 ttgtaagtgt gacaaaattt ccttgtaaaa aagataatct cgaatcatca tctttaaaat
    12601 aaagagtttc tctcatacct tctggcaatt ggtctataaa acaaaacata cacttatttc
    12661 tacagctttt agcttggtca attatcggat ttgtaaattc aacacccaaa tcttcatcat
    12721 agtctttttc tatctcgtat atgtagactt cgccatcttt tttttgtatt tctacatcta
    12781 aatattcatc acttagtaag aatctatact ctattatatc atgtattggc tgttcattca
    12841 cagatataag taaatctcct acctctatac ccatttcttc tgcaatacta tctttataaa
    12901 ccttacttat aatattattt acttttttta catttacttc caatattttc accactcttt
    12961 tgtattcatc aatgcttatt ataatgtatt ttaatatgca atttatttat ttattatatt
    13021 ttatgatagc atttttaagc atttcatgtc ataataacac attttaattc taatagtcaa
    13081 tataagtaaa tagctataaa atagattaga acaaataata ttcaaattta agtttcttaa
    13141 aaatttaaat attatttgtt cttaaaaaaa ttttataaat ttaatgtttt actatgatta
    13201 acgtcccatt tgtcatatca tgaaccattc cacctacaca ctttgacatc aacaatgcct
    13261 tattttgtaa gtctttctca tcatttgcat aaaatattgg acaacctcca ccatttttca
    13321 tattcttatc aagtgtaact atagctaggg tatattcatt taaacctata tccattccca
    13381 tcttatgcac ttcctttctt tcctatttta taatttttta gtgataagtt ttttccttta
    13441 gagcttgaaa gtattgggca tgactttaca gcttccatta ctttttccac attcttatct
    13501 actggtaaat aagcaatcat tatactttca tcctctttat ttcttctagg taatggtacg
    13561 aatgaaggtt ctccataatc tccatataaa cctaaccttg aatatatatt gtatactatt
    13621 gcttgtcttt gaccggagtc aaataatatt cctgcattag cataatcctt atttttagga
    13681 attatttgta ttcctacacc tctattaata tatacttctc tatcactttt tagacctata
    13741 tttgttattc cctttaaatc tcctaccttc aatatagact catctacaaa actaatctca
    13801 gcaggcatta catcagctat gtctccaata ctttttctag taagaagttt ttttaatata
    13861 aaggctaaca ctaatccaca aattgaactt attaccaaac tcattccttc acttattttc
    13921 gtttctgcaa gtattacata atacaatcct attgttaaaa aagatgtaac tatacacata
    13981 taatttctta cttcataggt tctagctatc tcttctatga atgcagctcc tctctttaca
    14041 agttgaactt cctctaagtt ttgcaatgta tttcttctat ttgctctcac ttgtctaaat
    14101 tgctctgctg ctagggataa aaaagttata gatgtataag aacgatttat aagagctgga
    14161 accaataatg ctcctaatga agaagcgacg aatgctaata taagttgaga tagtaaaaca
    14221 tttggttgcg ttggatactg tttttggtct aaattcaaca ttataattct agatgttata
    14281 ccaataataa cagcaacaaa aaatactcta tccattaaat cactcctaaa atatttaatt
    14341 tgaaatttta catgtaaatc tacaaagtct ttctagaaaa atcactctgc tttccttttg
    14401 atgtttctat gataggtgta cttttcacta catctatcat gacatctata tctttgagta
    14461 ttggcatata tggtataacc acagtacttt tttctaaatt ggtcttagat atagcaacaa
    14521 tatcaacctc atctacatct ctatctatcc ccatatgaat aaatagatta tgtattatag
    14581 cttgtctttg ccctatatca tttactattc caaatgcacc taaatctctt ggtataactt
    14641 ctatagccag acattcattt ctatactttt ctcttgtact ttctagacct atgttagtta
    14701 ttattacacc atttaccatt aaaataggtc cttcaaaact tatttttgca ggaactatat
    14761 ctgctatatc tgctatactg ttccttctta aaaatcttct aaaaatcaaa ccaacaatag
    14821 ctcctgacac aatagctaag attgtgcaat aaaaaaagct aaggccatac tttcttgcaa
    14881 aaacgatata aactatcgaa gataccagtg cagaaaaaag acttatataa cttctagatt
    14941 catacgtaga tgctatttct tcaacgtaag cgtctccttt tggtactaat tcttcattat
    15001 cgatattctt tagtgttatt ctttcttgtt ctgcaagacc ttgaaactgt tgtattgcta
    15061 ctgcaaaaaa tgtcaaggct gcaaactcct tatctataag tgcaggtaat gctatagcac
    15121 ctaacgaagc tgaaagccca gatataatta tctgttctaa ataatcttgt ggtcttgatg
    15181 ggtattgttt gtctgtaact cttaaaacta atcctctaca taatattcct acaactaatg
    15241 caactataaa agagtgtcta aataaatttg cattcaataa actttcttcc attttctcag
    15301 ctccttctct tatatagttt gtgcaaaaaa caaaaaaaat tcccttctat ttaaggaaat
    15361 ttttttgtaa tattgtaact ataaattcgc taccaatgcc aagttcactt tttactttta
    15421 tacatccatt tagtgatttg actatatgct ttgttatagc caatcctaaa cccgttcctc
    15481 ctacatccct acttcttgct ttatctaccc tataaaatcg ttcaaatatg cgttctacat
    15541 cttcttttgg tatccctaca ccattgtcta taactttaat tacgattttt tcttcatctt
    15601 ttaacactac aacttgtaca atcccattat cttgggtata ctttatagca ttatcaacta
    15661 ggtttaagaa cacttgcttt atatagtctt tattggatag tatacttatt gatttatcat
    15721 cacatttata taaaacttct atattttttt cactagctga atgttgagtt atttcatata
    15781 cctctttaaa tacatcatat actacaacat tttccataag aagagtatct ttattttcta
    15841 caaatgataa cagcaatata tcatcaatca acctttttaa tctatctgat tcgctttcta
    15901 ttatttctaa gaatctattt ctagtattca catctatatt ttcatttgac tttagtgttt
    15961 caacaaaccc acttatagat gtaagtggtg ttttaagttc atgacttaca tttgcaacaa
    16021 aatcacttct catattttca agtttaactt ttttagtaat atcttctata tttactatcg
    16081 aacctattat tacgttgtta gtattctgaa gataaactgg gtctactttt atcttatata
    16141 ccatatcatc tttagtagta atattagcag tttcattatc tttagaacca ataaattgaa
    16201 gtatttgttt taatatttct tcttctttta ttacaacatt aatattatga ccttcaacta
    16261 attgatttgg ttcactcttt atcatttctt ttgcttcatc atttatcaga agtatatttc
    16321 catttacatc tatagctaaa attccatgag atatactctt taatatagac cttagctgta
    16381 aatgtttata ctgtacttct tctaaggttt tatccatatt ctttatcata tgattaaaat
    16441 ttctagctaa ttcgccaagt tctcctttgg ctgatatgtt aagcctagca tgatattcat
    16501 tattacttac tttttttgag acgtatataa actcttttaa atatttcctt aatgttattg
    16561 tgtatcttac agctaatata acaatggcta tggacaacat aacaataaaa aaatatatac
    16621 tatttaaatc ttccacaaga taacactcct aaaatataac ctttttaatc tattttgtat
    16681 cctacacctc ttatagtctc tatatatttt tccgatttat cttcatcttc tatttttttt
    16741 cttaaatatc ttatatgaac atctactgtt ctagtttctc catagtattc atacccccat
    16801 attttatcca aaagataatt tcttgaaagt acttttcctt tattttgaat taataatttt
    16861 aacaattcaa attccttaag agttaactct atcttttcag agcctttggt aacttcatgt
    16921 tttgataaat ctagttttaa attacctgta gtaagtatat tgttttcttc acctaagcta
    16981 gtcaaattat atctccttaa aacagaacta atcctagcta acaactcttt tatactaaat
    17041 ggcttagtta tatagtcatc agcacctatt tcaagccctt caaccttgtc attttccata
    17101 tttttagcag taagcattat aacaggaata ttttttaagt ccttatcact tcttattttt
    17161 ctcaaaactt ctattccact tatatttggt aacatccaat ctaaaagtat taaatctggt
    17221 ttaatttcct tagcctttat aaatccgtca aatccatcat atgaataact aacttcgtaa
    17281 tttgacacct ccaaattgaa ttttaatagc tcaactatat gcatttcatc gtctataaca
    17341 agcactttag tattcattaa aaagctcctc tctattttat ctgtagtacc gttcctattc
    17401 gttctgatgc cttattatgt tttctataag agtggaattt atctgctcta caactagtgc
    17461 ataaatttaa atttattata ttttcttttt taactccact acatctaagc atatactcat
    17521 taattttcca taaatctaaa tagtaacttc cctcttttat tatatagaac ttttcatcat
    17581 tatttgtaag aattgtgtta aatttttcta ctaaatcttc agacacttca taacaacatg
    17641 gtccaattga tggcccaatt atacatacta aatcttcagg aactgtttta tagcattcag
    17701 acattaattt tattgtttta tgacctatat tactaaaagt ccctctccaa cctgcatgag
    17761 ccagtcctat ggctttattt ttagcatcta taattgatat aggtacacag tctgctgtaa
    17821 acagtaaaag tggtactttt tccaagtttg ttataagtgc atctccatct cttcttttcc
    17881 ctatagtatc tttattgact atattaacta tatctgaatg tatttgagaa ttactagtta
    17941 gattttcaaa tgtcaaatca cattctttac atacttttaa catatcttct ctattttttg
    18001 catctatatt cttacatgtt ataactagat ttacagaatt taatttttca tcaagttctt
    18061 cacaattacc acttaaatta acatactctt taatatcttg tacatttttt atatttatat
    18121 aatctttcat catttgtctc ctttttctat cagtatactt ttaagttcat ttacaaaagt
    18181 atttatatct ttaaattgtc tatacacaga agcaaatctt acataggcaa cctcatctaa
    18241 ctctttcaat ttatccatta ccatctcacc gatttttttg gtttcaattt cttctataaa
    18301 acatttattt atttcatttt caatatgtac aacaatactt tcaatctcgt caatagaaac
    18361 aggtcttttt tcacatgctt ttagcaatcc atattttatc ttttctctat caaaatattc
    18421 tctactatta tcctttttaa tgaccattaa aggagttgtt tctattttct catacgtagt
    18481 aaacctcttt ttacatgatt cacactcacg tcttcttctg atagatttta aatctgtatg
    18541 tctagaatct attacctttg attctttata attacaataa ggacattgca tctatattcc
    18601 tcctttgttt aactatatct caaactcatc taagcttaat tctgaaccta tatttactat
    18661 tattgtatca caacctattt taagtatatc attccaaaat ataacttctt catcccctcc
    18721 tcttgaaaaa aaacttctac ttccatctcc aaaaatggta aatgagacta ccttaccttc
    18781 gtgaatatcc atgtctatgt ctattataaa ccccatcctt tttccatttt ttacatttat
    18841 aatttctttt tccattatat cagaaactct aatcatattg tactcaccct ttctttaaaa
    18901 ataaatatgt atcgaagtaa aaaaaaatga cctatatagg tcatttaaat taaaataatg
    18961 tttacagtcc atggattact tatacatatt ttctcatatt ctttaaagca tttttttcaa
    19021 ttcttgatac ttgtgcttga gatataccga tttcatcagc aacttctatc tgagttcttc
    19081 ctttgtaaaa ccttaaatct aataccaact tttctctact atttaatttc ttaatagctt
    19141 ctttaagtga tatttcttgc aaccaatttt catcagtatc ttttttatct tgaacctgat
    19201 ccataacgaa tattgcatct ccattgtctt gatatactgg gtcaaacaga gatattgggt
    19261 cttgtatggc atcaagtgcc attacaactg attctacttc taattctaat tcctttgcta
    19321 tttctgatac tgtaggttct ttagaatttg ttctaattaa tctttctcta acttgtagtg
    19381 ccttatatgc tatatctttt aatgacctac ttactctaat tgggttattg tctcttagat
    19441 accttctaat ttctcctata atcataggaa cagcataagt tgaaaacctt acattttggc
    19501 ttaaatcaaa gttatctata gcttttataa gaccaataca acctatttga aataaatcgt
    19561 ctatattttc tcctctgttg ttaaactttt ttattacact caagactaat cttaaattcc
    19621 ctcttacaaa ctgttgtctg gcttcttcat caccattttt tatttgtaat aaaagttcct
    19681 tcatttgctt atttttaaga actggaagtt ctgatgtatt aacaccacat atttcaacct
    19741 tattaacttg catatatttt cagtcctctc tcaaaagtat tcatctattg aaattattta
    19801 catttccaat ttttttatac tttttaagaa gaattaattt agcattatac aaattttttc
    19861 atttcttttt gcaaccttga gattattttc ttttcaagtc tagatatgta ggactgtgat
    19921 attccaagca ttccagcaac ttctttttgt gttttttcaa tacctttatc aataagacca
    19981 aatcttaatt ccataatttg cttttctctg tcacttaatc tgtcaagtgc cataaccaac
    20041 aaatctctat ctatttcttc ttcaattatt ttgtatattt catcattttc tgtacccaat
    20101 acatcagata ataaaagttc gtttccatct aaatctatat ttaaaggttc atcaaatgat
    20161 acttctgttt tctttttatt attttttctt aagtacatta atatttcatt ttctatacat
    20221 ctcgaagcat aagttgccaa ttttatattt ttattcagtt taaaagtatt aactgctttt
    20281 atcaaaccta tagttcctat tgaaatcaaa tcttctacat ctatacctgt attttcaaat
    20341 tttctcgata tgtagactac taatctaaga tttctttcta ttaaaattga ctttacactt
    20401 tcatctgttt ctagcttttg taaaagctcc atttcttctt ctggtttcaa tggaggagga
    20461 agtatatttg cccctcccat atagtatatt ccctttggct ttattatttt taatttaata
    20521 cctaacatag taataaagct tattattctt tctttcaatc gtaacattcc ttgtccccct
    20581 taaattttca cctaaaatcc aggcaatata tttggattca ttattgctcc atattctcta
    20641 tcattaaaat ttgaaatacc aagtattata tttcctatct tttgctcatc tacttctata
    20701 tagtcagctt ttaagcctat tattatgctt gtcttattac ttccagcatg tttataaggt
    20761 attattctaa ctctacctga agttttatca tctaaactcc ctataatttc ttgtgctttc
    20821 ttcatatcta tatcttcata attaatattt aatagttttt ctggaataaa tccttctaac
    20881 acactagact taactattat tacatcactt tgacttattg ggtcttttaa caaattacca
    20941 ctatctacta aagctttaca gcaaaaactt ttttctagta agtttatatt tatagtcttt
    21001 gtaagctcct taatgtactt aagcattttc aaatctttat aaatgtattt taataattca
    21061 caactcacat aagtacatgc aattaagaaa gatattttta agtgacttat tcctgtaaaa
    21121 taaattataa aataagtgct tccagaaata aaaatgttta ctagataaaa aactaacgct
    21181 acatgaacat aatctttttt gctcttactt ctaaatgaaa ttaaagttat tatactcatg
    21241 attattattt taaatggtac agtaaatagt atattcattg atggatacaa ataagcaaca
    21301 gaataggcac ctcctaaact agcccccaat aacttatttt ttttaggatt atatctttta
    21361 gtaagaatag aagtacaact tataataata taatttatta ctaggttttc tataacatag
    21421 tactcaatat acaccatgta tcccccctta aaacctttaa acatattata agatagtatg
    21481 tacaaaattt tatgtcactt ttgatggtcg aatccaaaac aaattctatt taaaaagaac
    21541 aatgatatat gtcttatact ttatgtcgtc aaaagatatt tttttaatgc caaacaaata
    21601 gacttttttg tagaatccaa tatatctagg tagaagttat tcgctctact tttggtatat
    21661 ttacaaaaaa agactacata atttaataat atatcaagta aatcttttac ttatataaaa
    21721 tcaaattata tagtctgtaa attcaaatat aaattattgt gctagtaatc catttgcata
    21781 ttttgcagat tcatttagtg cttgttctgg agttatgtct ccagctatca tcatttgaag
    21841 ttgtttccat aaatattcat atatctctac tccatgaggt ccaactgtca atggtctata
    21901 catatgttta tctcttgtca ccatttcttc aaatcttggg tctccttggt atttttcacc
    21961 tttagcaact ggagctctag gtatttcttt gtggaaagct tccattgatt ctttgcttag
    22021 catatactca agaagtttaa atccaagttc tttattttta catgctgaca ttaatgttaa
    22081 atcatctact gaagcaaaac ttccaactgt cttatcttct aatgatgtta catatcccca
    22141 gtttaaatcc gggaatgatt tatcaaatgt acttgtagca gcagaggata gcattatagt
    22201 aaatgcagct tttcctggtc caaatacact ttcaatcatt tcattactat cttttgataa
    22261 agcatcttct ggaatatact ttttcatatc atttataaag tttgcagctt tcaagccttc
    22321 tgcattatta aatttaactg attttaaatc atcattatat atatctccac cagcttgcca
    22381 taaatacgga taccagttcc agtttaagct tccaaatact ttagaacccc aaccttgtgc
    22441 aagaccccac tggtcgattt ttccatctcc atcagtatct tttgttgctt tttcacagat
    22501 tcttttaaaa tcatcccatg tttttggagg tttttctcct aatttttcta gtatatcttt
    22561 attgtagtac aatacgcctg gattagcagc ttctataggt aaaccataaa taccacccat
    22621 catttttgca tcatctaagt atagataatt atctgaagtt cctgattttt ctaaataagg
    22681 tgttaaatct tctaccgcac ccatttctat aaattgagga aacatctctg catacatgta
    22741 tcctatatct ggaccttcac ctgcacttat tgctgttgca tatttttctg cgtagttatc
    22801 ccatggtact atctgaaatt caatttcaca gttattttct ttttcaaatt tttcaaatat
    22861 cggcttccat gcatctgcat catggtctgt taatggtgca gaccatacaa ctaatttttc
    22921 tttaccctct ttgcctttag aatcttcttt ttttgaacat cctgcaagaa ttgatactga
    22981 tagaatccct accataacta aagaaagaac cttttttagt ttaatcatgc ttaatcccct
    23041 ttcagacttt aatttgtctt tttcctcaaa tgtttacatt taattcagat ttaatattaa
    23101 caaataagat tactcttgtc taatcataaa aaactatact aactcctata actatatttt
    23161 ttttctatac ctattagatt taattcatac taaaaactta ctttttaaat tttatcaata
    23221 tttaaaaatt tttttaagtc tagccacaaa ctaactatct aaattgagca aaataaaaag
    23281 aagtctattg acttctttat aagcttacat attatcttct tcttcttagg aatgtaggta
    23341 tctccatgct ctcatcatca tcttcttcaa ttatacttgc ctttttagga ggctctattt
    23401 tttcctcagc aattacttct tcttcaattt cttttacagc ttgttttact gttgtattta
    23461 atgaagaacg tatgcttgat ttaggtttag tatctaaatc taaggcacca ttttgtaatc
    23521 cttcaaatcc tgtcgcgatt actgtaatca ttatttcatc acctaaatct tcttttatag
    23581 aagctccaaa tataacattt gcttctgggt cacaggattc cataactaaa gttgatgcct
    23641 cattaacttc aaataatcct aagttaggac cacctgtaac attaagaagt actcctttag
    23701 ctccttgtat tgatgtttct agaagtggag attgaatagc ttgtcttgca gcatcaattg
    23761 ctctagtttc accactagca ctacctatac ccatatgagc aagtcccttg tctttcatta
    23821 tagtagtaac atctgcaaag tccaagttta ttaacccttc aactgctata aggtctgaaa
    23881 ttgattgtat accttgtttt aatacatcat ctgcaactgc aaaagcatct aacattgatg
    23941 tatttttttg tactatctgt aaaagtctat catttggtat cgttataaga gtatctactt
    24001 ttgatttaag ttctgctatt ccaccttcag cgttcttcat tcttatctta ccttcaaatg
    24061 caaaaggttt ggtaactaca cctactgtaa gtatccccat ttcttttgcc aagcctgcaa
    24121 ccactggagc agctcctgta ccagtaccac cgcccattcc ggctgttaca aagaccatat
    24181 cagctccttg aagtaacttt actatttcat ctttactctc ctcagcagct cttttcccta
    24241 cttctggatt tgctcctgct ccaagtcctc tagttaattt ttctcctatt tgaactttat
    24301 attcagcttt tgatgtatat aaagcttgtt tatctgtatt tactgatata aattcaacgc
    24361 cttttagttg agcttctacc attctattga cagcattgtt tccaccgcca cctacaccta
    24421 ttactttaat ttgtgcacat tcttctaatt ctacgtcaaa gtttagcatc ataaaccccc
    24481 ttctctcatg attcaaagtt atcgtatgta ttaattatat cattttcttc ttttattttg
    24541 ttaaccttta agtaacatat tccacaaaaa cgtaaatttt cctttttaaa tttcttactt
    24601 atattgtcaa atattactct ttaagtataa aataatttac gtatatatta aatgctcttt
    24661 aatttttacg ttttttgttc ataatattac taataatata tcgtctaatt atagataagt
    24721 tattaaacaa tctcatacca aaaactaata cagcaacata gtaaagagaa actcctaact
    24781 tttctcctat ataagtcaat aatatagcca atatagcatt ccctatgaaa ccagtaataa
    24841 aaattacatt atcatagtta tcttcaaaat tggattttat cgctccaaaa atagaatcca
    24901 ttattgcaag aatagctact gacatatata tggaataatc catagaatat gtaaatggaa
    24961 tatagtaacc tactactaca ccaaatgcta atcctcctaa aacccataac ataaatcttc
    25021 acctctttat tttactttga aacacaacta cattacaatc ctttttatta atttttttta
    25081 ctttttcttt atttttttgt atttttcgtt ttattttgca aataaatcca ctttatttcc
    25141 attattttcc tgaactttta tattaattcc atatatatcc ttaagagatt tagcatatga
    25201 ttctggagag tttatagctg cactcaataa atctatatct cctatagctt ttattataaa
    25261 tggttgtcca tatgtagtat catttactgt aatagtagct cctgaacatt ttatcttact
    25321 tgttgctagt actctttctc catttataga aattgctctt gctccagcct tttttaaatc
    25381 atttaaaacc ctcaaaatat ctatatcatg tataatcaaa tcgtttggat tctgtccgtc
    25441 ttttaactct ctctcactat ccctcatagt aatagttatg cctggccctg taacagttga
    25501 ataaccactt aattctttaa cttcttcaag ctctttttgc ataagtgttt ggatagattc
    25561 atttccattc ttttttgctt ctttatattc attcaaagtt tttttgttgt ttttaagagt
    25621 ttcttttaaa ttttttattt ctttttttgt attttctacc tcactttcaa tactttcagt
    25681 ttgacctaat gttatgtata ctctatttgg gtctagtgat tttatataag ttgatatgaa
    25741 aacgcctaat atgaatgctc ctaatataac tattttcttt ttcatactat tatttcccct
    25801 ctgctttgtt tatatcacta tgaccattta tttctatatt gtcttctttg gtcactttta
    25861 ctttggcatt ataatatgtt tctatttccc agacaatacc atactttata ttaagtgccg
    25921 aagccaatgt atcagaatca ccaattgctt ttattataat tggttctttt atgacagtgt
    25981 cattcatata aagcttgtct tcttttaaat acatatatgt attagaaact attctctggt
    26041 cgtttataga tatggcactt gcttgtgctg aatttaattt atttatcatt gacaataata
    26101 aatcataatt atatagtata ctatcatttt tatcagagtt tcctaagtct tttgtagaat
    26161 ttatctcaat ttttatgcct gttccttcaa catctgtata tccagctaat gtttcatatt
    26221 tttctatttc tgacttaagt atgtcttgtt ctccttcatt gtttttgcct ctataagtct
    26281 ctatatcttt cttcacacta ttaacttctg attgtagatt ctctttttct ttttttaaag
    26341 atttaagttc ttctgtaagt tgctctcctc ttttaaaagt tgtcatccct ctattttcta
    26401 tattaataat tttaagttgt aaactaacaa gaacacccaa tacaatactt cctatgatta
    26461 tacttttatt tataaatctt tttttcaata tatcacttcc ttacaattgt ctatccttct
    26521 ggactataca ctgccaactt tccatagtta agatttatag tccctccatt agtattttta
    26581 ttttgcaggt caatcagaat tttacttacc ctagatatat tataatctaa gttatcatca
    26641 ttagaaagta agatttttat attacttctt gtaagcattt ctatattgct ttcttttttt
    26701 aagtttacat aatttatttt tctataaatt ccttcttctt ttaaataatt taaagtttta
    26761 aatacattct ccttagtttt ataattttta aattttatag atttattatc atcaatgcta
    26821 taatctacat ctactatcaa gtctttttta ctttcattag aacctcttag ttcttccaat
    26881 aaatttcctt ttttatctat ataacaataa ttatcttcat cttttaatac tgcaaatatt
    26941 tctttttctt ttaaacttat aattatctta ttgggaagtt ttctttttat ttccacattt
    27001 tctatatatg gattttttat aagtttattt ttcatatctt taaaattgta tgcaaaaata
    27061 ttttcattta gatttacatt tagttctttc attatatttg atttggtcac cctcttattt
    27121 ccaattacat ctattttctt tacatcaaat aaatcacttt ttacaattat acatatacaa
    27181 aaagataaca taagtataaa aactaaaact atcatgacat tattcgtatt taactttctt
    27241 ctttttttca aatataatca acccctttgg ttaataaata cacctttaga gcttgttttt
    27301 cctacaaaaa aattataata tttaaagtca aatttttgca ttttattaca taacatccaa
    27361 ataaggtatt aaattttata aataaatcaa tatttcatca aaa
//
LOCUS       DAEMIK010000041        26936 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEMIK010000041 DAEMIK010000000
VERSION     DAEMIK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.041"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..974)
                     /locus_tag="KPK87_001494"
     CDS             complement(<1..974)
                     /locus_tag="KPK87_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG2537651.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKE"
     gene            complement(1340..2734)
                     /gene="gltA"
                     /locus_tag="KPK87_001495"
     CDS             complement(1340..2734)
                     /gene="gltA"
                     /locus_tag="KPK87_001495"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG2537652.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            complement(2734..3627)
                     /locus_tag="KPK87_001496"
     CDS             complement(2734..3627)
                     /locus_tag="KPK87_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG2537653.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            4253..5104
                     /locus_tag="KPK87_001497"
     CDS             4253..5104
                     /locus_tag="KPK87_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG2537654.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNISNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(5237..6850)
                     /gene="ggt"
                     /locus_tag="KPK87_001498"
     CDS             complement(5237..6850)
                     /gene="ggt"
                     /locus_tag="KPK87_001498"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG2537655.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(7075..9012)
                     /locus_tag="KPK87_001499"
     CDS             complement(7075..9012)
                     /locus_tag="KPK87_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537656.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNKIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(8999..9766)
                     /locus_tag="KPK87_001500"
     CDS             complement(8999..9766)
                     /locus_tag="KPK87_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537657.1"
                     /translation="MEEILSAENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            10054..10728
                     /locus_tag="KPK87_001501"
     CDS             10054..10728
                     /locus_tag="KPK87_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537658.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            10752..11756
                     /locus_tag="KPK87_001502"
     CDS             10752..11756
                     /locus_tag="KPK87_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2537659.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            11868..13097
                     /locus_tag="KPK87_001503"
     CDS             11868..13097
                     /locus_tag="KPK87_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG2537660.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            13105..13779
                     /locus_tag="KPK87_001504"
     CDS             13105..13779
                     /locus_tag="KPK87_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537661.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            13782..15014
                     /locus_tag="KPK87_001505"
     CDS             13782..15014
                     /locus_tag="KPK87_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537662.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(15300..16565)
                     /locus_tag="KPK87_001506"
     CDS             complement(15300..16565)
                     /locus_tag="KPK87_001506"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG2537663.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(16618..17766)
                     /locus_tag="KPK87_001507"
     CDS             complement(16618..17766)
                     /locus_tag="KPK87_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG2537664.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(17866..18411)
                     /locus_tag="KPK87_001508"
     CDS             complement(17866..18411)
                     /locus_tag="KPK87_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG2537665.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(18634..19578)
                     /locus_tag="KPK87_001509"
     CDS             complement(18634..19578)
                     /locus_tag="KPK87_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2537666.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            19973..20854
                     /locus_tag="KPK87_001510"
     CDS             19973..20854
                     /locus_tag="KPK87_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG2537667.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(20915..22123)
                     /locus_tag="KPK87_001511"
     CDS             complement(20915..22123)
                     /locus_tag="KPK87_001511"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG2537668.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(22196..22448)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22600..23589)
                     /locus_tag="KPK87_001512"
     CDS             complement(22600..23589)
                     /locus_tag="KPK87_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG2537669.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            23741..24181
                     /locus_tag="KPK87_001513"
     CDS             23741..24181
                     /locus_tag="KPK87_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2537670.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            24508..24735
                     /locus_tag="KPK87_001514"
     CDS             24508..24735
                     /locus_tag="KPK87_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2537671.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            24761..26881
                     /gene="feoB"
                     /locus_tag="KPK87_001515"
     CDS             24761..26881
                     /gene="feoB"
                     /locus_tag="KPK87_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2537672.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIYAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
BASE COUNT         9331 a         4233 c         3341 g        10031 t
ORIGIN      
        1 ttttctttat tttttattcc acttaaagaa tcataataag ccaatttttt aagttgttct
       61 attaatttag cattacttaa tttccacaat atcataaata ttactatata aaaatacaac
      121 tttatcataa aattaactat tagattttta aagttcttaa atatattaca ttttgatact
      181 aaagtccaat tagtccactt tatgttcgta tattttacat tatataatct ccaattatcc
      241 caagataaaa aatcaccttg acatttttta tttatatttt ttaagatttc acttttagtg
      301 gttcctataa tattcccttc taaatcaagc atcttacttt tatcatttac atattttttg
      361 tttggatgag aaataatagt ataatcttta tctatcatta gaaatttaat agaatcatca
      421 aaattgcttc tacttattaa attatttaca tcttgaaaat aaaaagaagc aaaaatggtc
      481 ccaatcatct cattattttt tacaattggt acatatatga taaatacttt gtcacctgtt
      541 acattactga cttcaatatc tgaaactact acttgctttg ttttaattgc tttctcaaaa
      601 ctaggcttat cttttattga gttaacattt tccctatatg tacttgaagc atttcctttt
      661 gtatctgtta taccaatcat aaaaagatta tattcctttt gatatggact taataatttt
      721 cccttttcaa ttagtgatgt ttcaccatta gaaaatctat catcttgact aagagctttt
      781 aataaagata tgtttacttc taaatagttt tcaagttctc tttttgatgc ctcaatcata
      841 tttctagaag taatttccat agagttactt gcatatatga tggtacttgt taaatcaatc
      901 aaaactaata gtattaataa gattaatata tgcttaaaaa ctagcccatc aaatactttt
      961 tctctttttt tcatattaaa ataccattaa ttacacaatt tatttttcta ttatttaatg
     1021 ttagatttga caaatctgat tttattagcc acacattatt cactccttac aagaccttta
     1081 ttgtataact attttatcat agttagacaa agttatgtta cttagtatca aatattacaa
     1141 taataactga taaattatta aaccatctaa aaaagtggct acatcaaaat agagattgta
     1201 ctaagaccta aaagttagac tttctacagg aatgaaattt tcattgttga aatgattaaa
     1261 acatatttga ttttgtacaa aaagagtgcc tcatgaacta aaaagttcat tttgagacac
     1321 cctctcttta atttacaaat taagcattaa cttttgcttt taaatattca tctattgatg
     1381 ctgctgctgt tttaccagca cccattgcag atattaccgt tgctgctcca gtaacagcat
     1441 ctccacctgc aaatacaccc tctttagaag tctgaccatt ttcatctgtt attaagcatc
     1501 tcttcttatt tatatcaaga ctctttgttg tagaagatat tagtggattt gggcttgttc
     1561 caagtgacat tataactgtg tctacatcaa gtacaaattc agaaccttct atttctatag
     1621 gtcttcttct tccagaatca tctggctctc ctaattccat tcttatacaa accatacctt
     1681 taacatatcc attttcatct actaaaattt ccttaggatt tgttaatgta ttaaatataa
     1741 tgccttcttc ttttgcatga tgaacttctt cagctcttgc tggaagctct ttttcacttc
     1801 ttctataaac tatatatgat tctgaaccta atcttaatgc agttctagca gcgtccatag
     1861 caacatttcc accacctact acagcaactt ttttaccaga actaataggt gtatcataat
     1921 catctctata agccttcatt aagtttactc tagttaaaaa ctcatttgca gaaaatactc
     1981 catttgcatt ttcccctggt atattcataa acattggaag accagctcct gaacctatga
     2041 atatagcttc aaatccttca tcttctataa gttcatctac tgtaattgtt ttaccaacaa
     2101 ttacattagt ttctattttt acacctaatt ttcttatatt gtctatttca ggttgaacaa
     2161 cagcttgttt tggaagtcta aactcaggta ttccgtaagt taaaactcca cctggctcat
     2221 gcattgcttc aaagattgtt acatcatagc cttttttagc taaatcacca gcacaagcta
     2281 gacctgctgg accacttcct attactgcta ctttttgatt tttcttaggc tctgtatcag
     2341 ataaatttat gtcattttct tttgaccaat ctgctacaaa tctttctaat ttacctattg
     2401 atacagcatc tgattttatt ccaagtatac atacaccttc acattgactt tcttgtgggc
     2461 atactctacc acaaacagct ggtaatgaac tgcttttagc tattacttta gcagcacctt
     2521 ctatatcatc ttctttaatt tttgttataa accctggtat gtcgattcct actggacatc
     2581 cacccacaca ttttggtttt ttacaagcta aacatctatt agcttctgcc atagcttctt
     2641 ctttattata ccctaagcat acttcatcaa aatttgttgc tctcacagca ggttcttgtt
     2701 ctctaactgg tacttttact tttttagcat ccattatttg ttacctccac atccacaatt
     2761 actatgatga tgactatcgc cttcttcaag ttttagcata gctcttccct cttgagtctt
     2821 gtacatagac tgtcttctca ttgcttgatc aaaatcaact aaatgaccat caaattcagg
     2881 accatctaca catgcaaact tagtttcatt tccaacacta actctacaac caccacacat
     2941 acctgttcca tctatcatta ttgtatttaa acttactata gtaggtatat ttagttcttt
     3001 agttaattgg cacataaatt tcatcattat cattggtcca attactattg cttggtcgta
     3061 ttttttacct tgattatcaa ctaaatcttt taataggtct gtaactcttc cattaaatcc
     3121 atatgtacca tcatctgttg atacatatac atttttagct acttttttca tatcatcttc
     3181 taatataatt aattctttag ttcttgcacc aattattaca tctacatcta atccatgctc
     3241 tttaaaccat ttaacttgtg gataaactgg agcagaacca actccaccag ctacaaataa
     3301 tatcttttta tttcttaatt cttctatatc ttcatgtata aattcacttg cttgtcctaa
     3361 tggccctaca aaatccatta caaaatcgtt ttcttcaaac attgctaact ttttagttga
     3421 agcaccaact gtttggaata caatagttac tgttcctttt tctctatcat agtcagctat
     3481 tgtaagagga atcctctctc ccttttcatc atttttaatt attataaatt gacctggttg
     3541 agaagatttt gcaactctag gtgcctctat ttccattaaa taaatactat ctgttaattg
     3601 tctcttacta actattttat tactcatctc gctattctcc attctaacaa atatattttt
     3661 cccactttaa atttaattaa aaacataaac acacaaaact ataactcttt ataatataat
     3721 attagttctt ggttgattgt tttgtattgg acatcccccc tataataatt ataactgccc
     3781 aaactacaaa tagttgatat taacaaattt cttgtaaatg ccaataaaca ctaagtctat
     3841 atcatacgtc taatttgtta tagatttaac ctaatacaca cttaaagtat actatattat
     3901 gccaaatttg taacaaaaat tttataaata cataataaaa tttttttatt caattattta
     3961 tttaataaat taacttaggt tttcataata acactaattt ctttaaaaac taaacgtgta
     4021 taaggcttta atctattgca ataccagctt tcatattgta taaaatatac tcttatttta
     4081 ttatatatct aaaattttca atattcatta attatttgaa tttttatata aaatttttac
     4141 aatttctaaa gatttattta cttatgtaat aaattataat aatattagtt tataaagtta
     4201 ataattaata taaattaaaa taaagataat ataaacacag gaggtacatt ctttgaaaaa
     4261 cattatttca gcaaaagaac ttatatcaaa actagaaaaa aatgataatc tagttgtaat
     4321 agattgtaga tttgatctta tcaatagaac ttatggtatt gattcttata aaaaaggaca
     4381 catcaaaggc agttttatct tagatataga caaggattta tcatctccta caaaagaaca
     4441 tggtggtaaa aatcctttac aagacccttt aatactaaaa gaaaaattag aaaaaatggg
     4501 tgttgataat gacactacga ttgtaactta tgatgatgga gatttaaatg gagcttgcag
     4561 attatttttc caattaaaac atttggggct taaaaatgtc tatgtactag atgggggaat
     4621 aacatctttc gtaaaggaag gtggagaatt ggaagagaaa gtgaatatac caagttgtac
     4681 aggtaaggaa attagaccaa atataagcaa tgatttagtt gttccaatgg aatatgttaa
     4741 gtctaaatta tacaaaaaag atactgtaat aatagattgc cgtgctaatg agagatacca
     4801 gggtttagtt gaacctgctt actcaaaagc tggtcatata ccaagtgcaa aaaattattt
     4861 ttgtaaggat ttaataaaaa gtgactttga aaatggctca cttaaagata tagaattttt
     4921 aaaaaaattc tttaaagatt taaataatta tgatgaagta attttatctt gtggttcagg
     4981 gatatctgct tgtgttaaca gtcttgctct tagagaactg gatatacctc acaagattta
     5041 tattggtagc tttagtgact ggattagtta tgatgacaat gaaatcaaaa ctggtcaaga
     5101 ataaggctca taatctatat aaaatcagca tatgatagct taagttttgt aaaggcgtca
     5161 gtcattgtag actttcgcct ttacttatta tttcatatac tctttaaatt tattatatat
     5221 attttagttg ataatattaa taaactgcaa tatgaccatc acatcttgac tccgtcccac
     5281 aaatataact ttcctgttct gttttaaata ttatttgacc tcgtcccatg tcaacagtat
     5341 catgaattac ttttatttca tgaccctttt caattaatga atctattata tgctttggca
     5401 tctctggttc aacttctatt tcttttcctt ttatccactg ccatcttgga gcatctagcg
     5461 cctcttgtgg atttaatcca aaatcaatca tatttgttaa cacttgaata tgtccttgtg
     5521 gttgcataaa agctcccatt acaccaaatg gacctattgc tttatcttct ttacctaaaa
     5581 atccaggtat tatagtatga taaggttttt taaatggttt tactacattg tggtgtttag
     5641 ggtctaaatt aaaattattt cctctactat gtaaagcaat cccagttctt ggtatcacaa
     5701 ttcctgaacc aaaatttatg taattgctct gtatatatga aaccatattt ccatccttat
     5761 ctgcagtaca aaggtatact gtaccaccac aatatggctc tcctgcagtt ggatacaaag
     5821 cctctttatt gtcaatcaat aaacttcttt ttttggcata ctcttggctt agtaattcct
     5881 gaatctttac cttcatatgc tctatatcag ttacatatgt tttagaatct gcaaaagcta
     5941 attttaaact ctctataatt ttatgtatgt cttctacatt ttctatattt ccttctaatt
     6001 ccaattcttt taatatgtta agtgccatta aaactgttat tccatgacca tttggtggga
     6061 tttcaaatat tttatatccc ttatattctg tacttatagg ttctacccat gttggataaa
     6121 aacactctaa gtcactcttt cttatagccc cattaaattt tttagaatat tcatctattt
     6181 tatcagcaag tcttcctcta taaaagcttt cagcctgagt atttgctatc tcttctaatg
     6241 tacttgcctg ttcttcacat ataaatatat ctccagcctc tggagcttta ccatctttag
     6301 aaaatgtatc aaaccatggt ttgaactctt ctccaataag ttctttttca tagagttcat
     6361 atgatttttt ccatacctta gctacattag gagatactac gtatccctct cttgcatagt
     6421 ttatcgctgg agataagcat tccattaagc taagtttacc atatttttta tttaattctg
     6481 accaagctgc tggtattcca ggtacagtta ctgctccaaa cccatatttt ggcatttctt
     6541 tcatattatt ataatttttt aattccatat tttctggagc aaagccacta gaatttaaac
     6601 catacaatcg tttttcttcc tctatccaaa caagggcata tgcatctcct ccaatcccat
     6661 tgcttgtagg ttctactact gctaatgtgg cggctgttgc aactgctgca tcaacagcat
     6721 ttccaccctt ttttagtatt tcaagtccag cttgagatgc taatggtgtt gatgttgcaa
     6781 ctgctccatt tttagcgtac acgacatttc ttattgatga gtatttatgt tggtaggcat
     6841 taaacttcat aaatttcagc tcctcgtcat tttttatttg atattctgct tagatttatt
     6901 atataacatc aagtaatgta actgatacaa aatgatataa aatatagact ttttcctaac
     6961 tttaatttta tctttaaata aatatcttaa actcaattct agtattttct atctgcactt
     7021 aaataagtta ttttattcaa aacatatatc taattctttt gacttaaaaa taatttattc
     7081 ttttatcatg tttagagcat attttaaagt taaaaaataa tatataaaat atcctataaa
     7141 ataaatgact agagttatga taattggtac aagtaatgac cttggtataa acttttgtgc
     7201 tatagacata gcaaataaat tatgaactgt acctacaact aatggcatta aaaatataac
     7261 ttttaactgt tttgatatta ttttctcaat atctttatta tttgctccaa gttttattaa
     7321 cataatatat ctttccttat catcatatat atttgataat tgtttaaata agattatact
     7381 acctgtacat aaaagaaata ctacagacaa aaatatgcct ataaacagca ttagaccact
     7441 taattttatc aagttattat aactttcaat acttgatgta aaattaaaag gataagtaaa
     7501 tttcatattt tcacttacta tattttttaa ttttaaagtc aaatccagtt cttttttttc
     7561 attttttata tcaattaatc ttattaatct agaatttcca tgtaatttta actgattaaa
     7621 tacgtcatct tttactacaa ttaaatcaaa cgtagcttgt gaatttattt ctggctccaa
     7681 gatgcttttt tgtactttaa acagatttat atcatactca tttgtatctt taatatgcaa
     7741 gtttattttt tcaacctcaa agtctttaaa aaatattttt ttgtatgaat cagaaacata
     7801 gtacacagat tcctttgaag ttaattcttc ataattgtta ttctgatatt gtcttaattt
     7861 tttaaaatca ctttctctaa taatatctac agacgttcta tcttctatat aactatttcc
     7921 atttttataa tacatttcaa tatttatatc tttgtctata agttctattg ttttatcaaa
     7981 gattatctta ttatcttttt tatatttata taataatttg tcaattttgt tatttacata
     8041 agaatttcct aaatttatgg tataactgta tttataattt tcacttatgt tcctatctat
     8101 atcgtaataa agtgatatgg taaaccctag catagttaca cttgtagcta ttaatattgc
     8161 tattacagcc aatacactac tattgctctt taatttaaat ccaagttcag aaaatgcaat
     8221 taaattctgt cccttataat aaaaactttt tctcttctta acagcatcaa taatcatata
     8281 catcgttgat gaaaaaaata aaaaagttcc aggtattatt gtaactaata taaaaactga
     8341 aagcataata ttttcagcaa taaaataact aattgacatt aaataagaaa tagctattaa
     8401 taaaacacca agtacacatt ttaactttat aaatttttta aacttatact tttctggata
     8461 tttattaaat aactctataa ttctcttatt tctgataact atagtatttc ttattccaat
     8521 aactataaat attattaaaa ataccgctag tgtctgaact aaggccttta tacttaagtt
     8581 catatttact actatcattt cattcattaa ttttacaagc atcatagtca taagctttga
     8641 aaataacagt cctaaaaata tacctattag tattgcaact atacccatca aaaaagtttc
     8701 atataaaaat atttttccta tctgattttt cctcattcca agtaaactat aagttcctac
     8761 ttctcgccta cgtctattga aaaaaaatga gtttgaataa tatataaata aaaatacaaa
     8821 taccactatt acaattgaac ttgtattaat actcatcctg gttttctctc caagtgaact
     8881 caacgcttca ttatactcta tagatttaaa actaaaaaat acaaatacac taaatacaat
     8941 tgaaataaag tacattaaat aattaaaaaa actattcttt acattttgca ttgcaattct
     9001 aaataaagtc atttttatta actcctccaa ttaaggaaac tgtatttact attttattat
     9061 agaactctct gttactttct ccctttacaa tttctgtgaa tattattcca tcctttataa
     9121 agagtattct attgcaaaaa ctcgcactta tagcatcatg agttaccatc aatattgttt
     9181 tattgctgtc tttgtttaat tttaatagac aattcaacaa atccctagca gatttagaat
     9241 ctaatgctcc tgtaggctca tctgcaagta ctaatgatgg ttttgttgct atagctctac
     9301 atacagctcc tctttgcttt tgtccaccag aaacttcgcc aggatattta tctaatatag
     9361 attctatatt taattctttt gaaatctctt ttattctatt ttctattact gatactcttt
     9421 cattttttaa agaaagaggc aacattatat tttcttttat tgtaagtgta tccaaaagat
     9481 tagaatcttg aaatacaaag cctagattat cacgtctaaa atctgccaat tcattttgct
     9541 tcattttaat agtattcttt ccatttatat aaatatctcc tgatgaaggt aaatctattg
     9601 tagaaataat atttaaaagt gttgatttac cagcaccgga agaccccata ataccaacaa
     9661 attctccttt ataaaccttg aaagttattc ctcttagagc ttcatgtttt gaaccttttc
     9721 ttccatactc tttttttata ttttcagcag ataatatttc ttccatagtt aataatcacc
     9781 tttcatcaca attagttatt tatcaattct cacaaatata ttatatatat taaaatggct
     9841 tttaaacatt taattatatt aatttaaggt tacattttcg taagtattaa ttatatttct
     9901 tcaaatttat tgcaattatg tttaaaatat aatttatcaa gtaaaatata gttatattaa
     9961 tataattatt ttaggtaata aggtagaaat attttaattt atcaagtaga atatagttat
    10021 gttaatataa ctattttgaa ttaggaggta agaatgttta aaataatggt agttgaagat
    10081 gacgtctcat tgaaaaatat catagcaaag tgtcttacta aatggggaca tgatgttcat
    10141 caaatagaaa atcttgaaaa tataatagaa gagttcaaaa actataaccc agaattagtt
    10201 ttattggaca taaaccttcc attttatgat ggttttcatt ggtgtaatga aataagaaaa
    10261 atttcaaaag ttcctataat ttttatttct tcaagaaatt ctaatatgga tgtaataatg
    10321 ggtgtaaatt taggtgcaga tgattatata caaaaacctt tttctgtaga tgtcttagta
    10381 gctaaagtta atgccctttt aagaagaact tataattttg ttgataataa ctcaaatcag
    10441 attattcaca atggtgttac tttagattta tcaactgcaa caataaacta tgaagataat
    10501 actattgagc taacaaaaaa tgaaatcaaa attctccatg agcttatgaa atataagggg
    10561 cagatagtaa gtaggaataa actcatgaaa aaattatggg ataatgattg gtttgtagat
    10621 gataatacat taactgtcaa tgtaaataga atacgctcaa aattaaatga aattggttta
    10681 gaagatttta tagaaacaaa aagaggattg ggttatataa tcagttaaaa caattctaag
    10741 taggtgaatt tatgagcttt aaagattttc tcaaggataa actaggtttt ttagtataca
    10801 attttacttt cttaattttt acagtatcag ttattatatt ttctcctgta gatgtttttc
    10861 tgactgatac tattttatat atattagttg taaatgttgt gtttttattt ttatatctat
    10921 ttattagcta catcaagaag aacaaatttt tagataagat aaaaaatgat acattcaaaa
    10981 ttaatatgaa tgatatagaa ttagctagat gtaaaaatga agaaaaaata tacttgaaca
    11041 ttattaaaaa tcatcaatat caatgtaata acataataaa taataatatc gagaaatttg
    11101 aagaaaacaa agagatttta aatatgtggg tacatgatgt aaagatacct atagctataa
    11161 taaaactcat tcttgaacaa aatgaaaatt taattgatga aaaaatttca gatgacatag
    11221 atagtgaaat atttagaatt gaaaactccg ttgaaaaaat tttatattta tcaagactcg
    11281 aagattttca taaggatttt ttaattcaag aggtaaatat tgaaaaaatc attagaaata
    11341 ttataaaaaa atactcaaaa tattttattt caaaaaaaat tattttagac ttaaataatt
    11401 tgaattttaa ggttttatct gatgaaaaat ggctatactt catattcgag caattattga
    11461 gtaattccct aaaatactcc tcattaggag ataatatatc tatctattgc aaaagaacca
    11521 aaaattattt acaattaaga gtggaagata ctggttgtgg tataaaaaag gaagatttaa
    11581 atcgaatatt caataaggga tttacaggta ataatggaag aaacaattct aaatctacag
    11641 gtcttgggct ttatttagta aaagaacttt gtgataagct tggtcataaa attgatgtag
    11701 attcagagta caaccaatat acaaaattta ctattacatt caaatgtgat ttatagtatc
    11761 ttattattga tagtttattt tcattaactt atattcaact tcttatagta attctatatg
    11821 tacatcccag tatataatta agtagttttt ataaaaagga ggaagacatg tcttttctaa
    11881 aaaaatttaa aaataaaaga tttagaaata acagttttaa aagtaacaac tttagaaata
    11941 gcagttttgg aagtagtggc ttcaagtcta aaaataaagg ttttaaaaac aatcccttta
    12001 aaaataagag attcaaaata tatgcaggaa tagcagtaat catactttta attctaggag
    12061 ttcttgctta tgttgattcg aaaaacactg aattacaagc taatgaaaat tttatcgaaa
    12121 catatactat tcctgaaaat gaaaaaatat ttataaatgg gatggttgtt cctaagcaaa
    12181 ctaaggactt caatatatct ggagattatg agttaagtga tgttaatgtt acaaatggtc
    12241 aaaaagtaaa tcaaggagac cttcttttta ctgctaaaaa tccaactata atagcagaaa
    12301 tagatagttt aaaatcacaa cttagtcaat acaaaaaaca aaaaatatca ctttctgata
    12361 ttgctgaaaa taaagatgct attgcttcta taaatgcaca gataactgct ttaaactctc
    12421 agatagcttc tctggataaa aaagcttatg acagagttac tgctcctttt gatggcacag
    12481 tatacttgaa tgaccaaact ggaaatccag accaaccagt ttcatttatg actattcaag
    12541 gtctagagtt ttatatgaaa ggacaagcaa gtgaacaaga tttacctaaa atgaagattg
    12601 accaaatggt caatatacta gttttttcaa ctgaccaaaa gcttactggt agaataagtt
    12661 ttatatctga taaaccatct actccaaata ctgaaatggg agctcagcag aatacactat
    12721 cttattatga tataaacata gcatttgata atcaagaagg attagtaaat ggattccatc
    12781 ttcaagcatc acttgaagta gctaattcaa gctttaaaat accagcatct tgtgtactta
    12841 aagacaacaa acactcatat gtatttaagg atttagatgg tatattgaaa aagcaaattg
    12901 tagatgtagc aagtcaaaat gatgattttg ctgtagttag aggcggtctt gaacaaggcg
    12961 atataataat aaaacaccct actaaagaaa tgaaagaagg agacccagtt caaggcgatg
    13021 gcgtaactgc tggttctaat aatggagata ctactccaaa caaaaaagta gaagaagtta
    13081 ctatggatgt gaattaattt acatatgtta attaaattag aaaatattca aaaatattac
    13141 aaagtaggga aagatgaatt acatgtttta aaatccttaa atttagaaat tgaatctgga
    13201 gaatttgtca tgataatggg taagtctggt agtggtaaaa ctactttact taatatctta
    13261 ggttttctag atgtatttga tgaaggaaga tatatatttg atggaacaga tgtaactaat
    13321 ttgagtgaaa atgaacgttc tgtttttaga aatattaata ttggatttgt tttccaacaa
    13381 tttaatttaa tagaaactct aaatgtatat caaaatgtag agttgcctct aatttacaat
    13441 aaagcactaa aaaaatctaa tagagaagaa attgttaaag ataaattaag ttctgttggg
    13501 cttttggaca agcttaagca gaaaccactt caattatctg gtggtcaaca acagcgtgta
    13561 gccattgcac gttgcctagc aaatgaccct cagataatct ttgctgacga accgactggg
    13621 gcattagata gtgaaactag cagagaaatt atggagcttt taacaaggct caacaaacaa
    13681 ggtaagacta taataatggt tacacatgat caagatttaa ctaagtatgc aactaaggtt
    13741 atacgactta aagatggtgt gtttacttcg gaggtgtaat catgagttta ttaaaaaatt
    13801 ctttggccaa tttaaaaggt cacaagcttc gtgtatttgt agctttactc tggattataa
    13861 taggaataac ttcagtaata cttgttagtt ctataggtaa tggatttcaa aaagaaatta
    13921 agaagtctgt aaacaatgta aaccctaata aaactactat ttcgtttgaa tcagccgata
    13981 acacaggtct tactgatgat atgagtattt ttttaaaacc ctttaatgca aaagatttag
    14041 aagaattatc atttgttgaa ggagtagaaa gaatagctcc ttcaagagat gggtttaacc
    14101 ttgattcagt ttactcttca caagcatctt ttgataaaaa aactacgtat gttgatgttg
    14161 gaccagtaaa aaaagattct aaaataaatt taatttgtgg tagagatttt tctttagatg
    14221 atgaaaaaag aaaagtaatt ttacttacat tacaaagtac aagtgaaatt tttgaaaatc
    14281 cagaagatgc cttaggtcat ggtatcaaca taaatggaac tatatttgaa ataatagggg
    14341 tattagatga ttctcagcaa aaccaagctg gaggattctt tggaggttac caagatatgc
    14401 aatttactac ttccctagta cctaaaaagg cttttgacac tttaatgagt caaaattctt
    14461 attcaaatga aatttaccag ttagacttag tttcttctaa aggctataat gtaaatgaag
    14521 ttgcaaacaa tgttatagct aaattatatg agatgcaccc tggtatcaat ggttcttata
    14581 ctacaccaga cccaactgaa cagacagctt atcttgaaag cattaattcc aatgtaaaca
    14641 aatatgtatc tataataact gtagttgcaa tgtttgttgg tggtattggt gtaatgaata
    14701 taatgtatgt atctgttatg gaaagacaaa gagaaattgg tataagacgt gctattggag
    14761 caaaacctag gtctatatta tttcaatttt tagttgaagc tgtttttata acagtatgtg
    14821 gtggaatttt aggaactata gttggatttg ctgctactaa ctatgtttct aaatatatag
    14881 gttttgaagc tatacctagc ttaaatagct tattttatgc aattgtagct actatattga
    14941 ctggagttgt atttggtctg attccagcct ttaaagcctc taaactagac cctataaagg
    15001 caatttataa gtagaaattg tataaagatt ttcgattttt agattagttt atataaaaca
    15061 tacttaaata tataacgcac tattatatat gatagattag gaacttaatc tttccaaata
    15121 taatagtgcg ttctttcata aaaaggattc tcttccttac tatatctaaa ttaccaagat
    15181 gttatgttct aattgttcaa actaatactt ccaattttga ttaatcaatc tcattctcct
    15241 aatatacaat cttttacttt caatcattgt acactccgcc taaaaaaaat aatcttaaat
    15301 taggcgttat atacaatctc cccattacat attgtataat taacctttcc atataaagat
    15361 ttacctttaa atggtgaatt atcagcttta gaaacaaagc tttcaactgt ccattcttct
    15421 tctggattga atataacaat gtctgctaca gccccttctt ctataaatcc aatatttaaa
    15481 ttgtacaact ttgcaggatt tatagacatt ttttctatta actgcatcat actaagatga
    15541 tttttatgca ccaaatttgt tattccaaga gctaaagaag tttcaagccc aataattcca
    15601 cttggagctt ttataaactc tctatctttt tcttctttac tatgaggtgc atggtctgtt
    15661 gctattattt caatagtacc atcatttaat ccctctataa tcttatctct gtcccattta
    15721 cttcttagag gtggattcat ttttgcattt gttccaaact ccaatacatc ttcctcagtc
    15781 aatgtaaagt gatgtggtga agcttctgca actacatttg cacctaagta ttttgcaaat
    15841 ctaaccatat caacagatac acctgaactt atatgttgta tatcaacttt agcaccagtc
    15901 tttagtgcca acatacaatc tctggctacc ataacatctt ctgctacact tgatgcacct
    15961 tttaatccaa gttctgaagc tacctttcct gcatttacac ctggatttcc aactaaagaa
    16021 gggtcttctt catgaaaact aagtggtaca tccaactctt ttgccttaat cattgcctcc
    16081 ataattagat tgctatccat taaaggtaat ccatcatcag taaaccctgg tacaccagct
    16141 tttttaagag cttccatatc aactaactct ttaccttcaa aacctttagt tatagctgct
    16201 gcttgtaaaa catttataca tgcatttttt gacttttctt taacataatt aaaagtatct
    16261 tcattatcta caataggatt agtatttgcc atacatataa cagttgtaaa tccacctcta
    16321 gcagcagatt tagctccact ttctatatct tctttatatg taaacccagg gtctctaaag
    16381 tgtacatgta catccactaa acctggagca actacattac cactagcatc tattattttt
    16441 tcatattcat cactttcatc aaacttgcct attttataaa ttttattttc ttttataatt
    16501 aagtcaatat tctcatctct ttgactttta gggtctatta ctctaccatt tttaattaaa
    16561 atcatattat ttctccttta tatttttaat ttatatagac tcatataata ttatacatta
    16621 ttttacatac ttaaatacaa aattacgttt gacatcaata gcctttttag gacaaagttc
    16681 atggcaacag taacatctta tacatttact taagtctata atagcaccct tagtccctat
    16741 ctctatagtg gatgcaggac aagcttcctt gcattttcca cattttatgc aatcttgaaa
    16801 tcttaccaca ggctttggtg ttataagctt tgaaataggt tcatttaaaa attttggtag
    16861 ctttacggaa aacaatctta aatctttact tacatttgga atcttgtaat tttttataat
    16921 taaatctttt atatcttcac ctaaaatttc aatatctgaa aaatcttctt gtatatatcc
    16981 tctcttaata gcacctctaa ttgtagcaac tctatttgga agtaaactta taatcttaca
    17041 tgcaacaaca tctatagcat atggattttg tgatgctaat aaaactccta tttctcgagt
    17101 agtaccagct gaaggtccat caccttccat acctactata ccatccataa ttgtcaatgt
    17161 tggttttata taatcacaga tatctaaaag tacattttcg caaaacaact cttctgtagg
    17221 aaatctataa tgtatctctg cttttttcaa tccagctatg catccatata agttttttgt
    17281 accaccagta aaagttgcca ttatatgagt ctttaattta caaagattta ttatataatc
    17341 tgaatctact attggtttaa taatatccat atattttaga acatgtgctt tttcactatg
    17401 tatcttaaca tctgaaacat cataatttaa cttaatgccc agttcatttg ctacttcttc
    17461 aattccagat gccttataaa tgctcttaag agaattttga ttatatggac caccagggct
    17521 atctgctatt gtaatatcac aatttaactc taaaagtttt tcacatacaa cttttacaac
    17581 cataggatgt gtagttgtag cttcttcagg cttttttttc atcaataaat ttggttttat
    17641 aagtactttg cttccttttt taacaaactt atctatacca ccaatatcct ctaaattttt
    17701 ttcaatagat aactttactt cttcataatt gtaagtttta caacttctaa cagataccga
    17761 cttcatatta tctccatcct tctatcacaa cagtttattt gtataattta aaaaaggaga
    17821 aaccaaagtt tctcctttta atctatgttt taattgtttt aattattatt ttccaaaatg
    17881 tctgtttaat aatcctaaga atgctttacc atgtctagct tcgtctttac acatttcatg
    17941 tactgtatca tgtatagcat ctaatcctaa ttctttagct ctcttagcta attttaattt
    18001 tccatcagtt gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc
    18061 aactacaact tctccaagaa gttctgcaaa tttagcagca tgttcagcct cttcaaaagc
    18121 tattctctta tacgcttcag ctacttctgg ataaccttct ctatcagctt gtctactcat
    18181 tgctaaatac attcctactt ctgtacactc accagtaaag ttggctctta atccttcgat
    18241 tatttctgca tctacacctt gagctactcc tattctatgt tcatcagccc aaaccatttc
    18301 gcctttcatt tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc
    18361 agcatctcct tcatgtatat atccacatac tgtacaaaca aattttttca taattaattt
    18421 cctcccaatt ttatataatt aaatttttat ttttaagttt aataacttgc tacaagaata
    18481 tatatacccc agactttata gcttaaacac taaaaataaa atttatttcc taattaattt
    18541 tatcactttg aaacaccagt atcaaccaag ttttgatgtc aaatttctac ataatacata
    18601 atttatttga ttaattagga aatagttgtc atattaaata ctttctaatg aagactcctc
    18661 tgaatcttta agtttcactt ttttgctctt taaaaaagat aatttagttt ctgcagttat
    18721 gattgcaaca aatatcaaga tactaccaat aaccaatttt gctgtaataa gttcctttag
    18781 aattattata gaaaatattg taccaaatac agcctctgtg gataatatta tagctgttct
    18841 agttccatca actctttttt gacatatagt ctgaaataaa aatccaattg tagtactaaa
    18901 tacagctaga tacatagttc ccatgtaacc agataattca gcttccatct ttaattgacc
    18961 tgcaaatgtt tgaactacaa cagacattaa aaacgctact gtaaattgaa tagctgtcaa
    19021 tgccattggg ttgttatctt ttgcaaactc acttgtaaag aatatatgaa atgcaaatcc
    19081 gaatgaacat ataagtgtta gaaaatctcc aaaattaata gagaaatcag cttctaaaga
    19141 aagtatccca attcctataa gagctaccaa actacttatt attcctattt tatcaagttt
    19201 tcttctataa agtataaatc ctatgaacgg aactattaca acattagctg ctgttataaa
    19261 cgcattttta gatggtgtag tgtacataag tccaatagtt tgtactgtga atgctagaaa
    19321 taaaaatatt cctaaaatcc ctcctgcttt taataatttt ttacccatat tggcacgaat
    19381 ctgtttaaaa aacagtaaat tcataattat agctgccaag aaaaatctta aagttatgat
    19441 ttgtagaggt gtaagcccac cgtctaatgc aaattgggtt gctacaaagc cacttcccca
    19501 tataattgct ataaatatta gtcctatttc tccaaagtat tttttcaata aagccccctc
    19561 ctttttattt ttttgcaaaa ttataaaccc cctaaacagc aagagagttt ttctataagc
    19621 attactataa attaatatag taaatttttt cagtatttta aatctaaatt tacatacatt
    19681 ttgagctcaa atttatatcc actatttaac agtaatatat taatcatttt ttgtaaacac
    19741 ctttttaata aatttttata ttttttatat tttttatatt tttttaccta tactaaattt
    19801 ttttatagat atttttaata tatatgatac tttaatatta tgcaaataat gtcatataag
    19861 ttacacttta gttatcttag ttacaacttt gtcggtttac tttttctact ttcttcgata
    19921 taattataat atatttttga tacaattcga caaaaggggg aatgctgtaa ttatggtaaa
    19981 aaaaagaaaa aagaaaactg ttacacttac tatacttgtt gtaatagtta ttggtatttt
    20041 ttctatggca tgggcggaca tgtctagaaa aaaagaaatg aaagaaactc aagaaagact
    20101 taaagctgaa agattagaaa aagaaagaat tgaaaaagaa aaaaatgaac ctattattgg
    20161 tgcaaaaaaa gaagctgtaa aagaatatgg atatgacgca aaaatagttt gggacaaact
    20221 taacaaatac gactattcaa acaatggaga aaagatagtc tttcttacat ttgatgatgg
    20281 accatctact actaatactc cacaagttct agatatcttg aaaagacatg gtgttagagg
    20341 aactttcttt attaagggtg attctcttga aagaaaagga gctaatgaaa ttttaaaaag
    20401 aacatttgac gaaggaaatg caatagccca tcactcttat actcatgact ataaaaaact
    20461 atatcctaac agaagcctaa atctcgatac attcgtaaat gaacttaaca aaactgatga
    20521 agctatgaaa aaagtcttag gaaagaattt ttcctcaaat gtagttagat gcccaggcgg
    20581 gtatatgtca tggaaaaata tggaacctct aggaaactac ttaaaagaga aaaacatggc
    20641 atcaattgat tggaacgctt taaatgctga tgcagaaggt aaaaagaaaa atgcacaaga
    20701 attatttgaa catgcaaaga aatcttctga aggcaaagaa atggttgttc ttttaatgca
    20761 tgatacttat ggaaaacaag aaacagtaaa tgcattggac caaattataa catattttaa
    20821 agacaatggt tatcaattta aaattttaat ataataaaaa agcaagttta ttgaatgtac
    20881 attttatact caatagactt gcttttactt tactctattt tactagctcg atttttacaa
    20941 attttttctt tcctatttga acaactaatt cactctcttt tacaatagtg cttaaatcct
    21001 ctactttttc accatttacc tttactccac cttgagaagc taatcttcta acttcactct
    21061 tactcttaac aatctcatta gaaactagta cttcgattaa gtcaaatcca tcttccttaa
    21121 cttgaattgt ttgtatatct tcaggtattt gacctttttg gaatacagat ttaaatcttt
    21181 cttcagcttc tttagcagat tcttctccat ggtataatgt tactatttct cttgctaagt
    21241 tcatctttat atctcttgga tttactgaac cctctttcaa ctgagattct attttattaa
    21301 cttcatcagg atgaacatct gtcactaaat tgtagtattt aattataagt tcatcaggaa
    21361 tttccataga tttttggtac attattcccg cttcttcatc tattccaata tagttaccta
    21421 aactcttact cattttttct ttaccatcta atccttctat taaaggcatc attattacta
    21481 tttgagactc cataccaaat tctctttgta atgatcttcc cattagtaaa ttgaatcttt
    21541 ggtcagttcc accaagttct atatcagctt ctaatgctat agagtcatat gcttgcatta
    21601 atgggtagaa gaactcatgt actgatattg gcatttgtcc ttcatatctc tttttaaaat
    21661 cttctctttc taacattctc gcaacagtaa tagttgctgc taattttatt acatcttcaa
    21721 aatttaactt agctaaccac tcgctattaa atcttacaat agttttttct ttatctaaaa
    21781 ctttgaaaat ttgttcttca taagtttttg catttgcaag aacttgctct gttgttaaag
    21841 cttttctagc tttagatttt ccagttgggt ctccaatttt tcctgtaaaa tctccaatta
    21901 ttattactat ttgatgacct aaatcttgta attgtttcat ctttctaaga actactgtat
    21961 gccctaaatg tatatctgga gcacttgggt ctagacccaa ttttactatc ataggttttc
    22021 cttctttttc tgatttaata agcttttccc ttaactcttt ctcatctatt aaatcatcta
    22081 cacctttcat aattattcgc atttgctcgt ctatcgattt catttttcat tcctcctaaa
    22141 atatttatat aatctataat ttttattttt ataataaaaa aagctttcat cctcttaaaa
    22201 ggacgaaagc attgcttacg tgttaccacc ttagttcatc aatatctcac gatattaatc
    22261 tcttagagta caattacata tactcgtgta ctataacgtg tactaatccg atatctctta
    22321 atggttgtta tctttcagag atagtgctct gagatgtatt caaactaaat caatttattc
    22381 catttcacca aactggaact ctctgttaaa taaaatatag ccctacttct tctcttcata
    22441 gcatttatat attcagatta tttcatcaat tacaagaaat ttttcttaac ttactcataa
    22501 tatattatta tttttatatt ttgtcaacct tttttattat tatatacatg tttttttaca
    22561 ttatactact tttttatatt ttttaccaat acccaaaatt tatttttcaa tcaaaagatt
    22621 tatttgttta ttgactatat tactctgact caatcttata tttattttct tatttaaaaa
    22681 tgcgtttata attattaaac caataactgt tacatcaaat ataaaaaaca gcaataaaat
    22741 tattatcaaa aataaaattc tagcatataa taattttcta aacttagcta ttataaagtt
    22801 ttgtagtgac atacatgagt ctctaatgat aaaatagaca cttatatagg ttattgatat
    22861 aaagattgtt tttaaataaa cagaatcaac tttaacattc aacagtttta gaacttcaaa
    22921 aatggctaga taagaaacac atatgtaaaa agcttttttt gagcaacata aaaattggtt
    22981 taaatttcta aaattcttaa atattaaaaa tttttttaaa gaattagaat ctaaaatatg
    23041 caatttattc cctaaaatta aacttaataa atatatgcaa aacaccatac caaaaggaaa
    23101 caatgctatc attaaataat aaataaaatt tgtataccca ttaaaataaa tccatttgct
    23161 atatccttga aattctttca taaaactata acctattaat gttgaagcat atatatcaat
    23221 gaaaaccatt acaataaccc caaatactga accatatact aaatcatcca taaccattgt
    23281 aggcttattt attagatagc cacacattat accaagtaat acactctgac ttgcccaaat
    23341 agcagttcct agatttccaa gtaccagaga aattattaaa agcgaagtaa ttccagataa
    23401 aatggtatac tttaaatcac atttcaagca gattatacaa aaaaatactg gcactataaa
    23461 atctaagtaa atagcatatc caaatccagt actcacagct ataattgtta ctactatgaa
    23521 taaagatgac aataaagctg cttcagttaa ttttttagta gtattcaaaa cactgcctcc
    23581 ataaatcata aattttctaa ctaaagtttt gtttttaata tagtatatca ctttttaaaa
    23641 atatttttaa taaatataaa ttttaataaa ggtatttatg ttaaaaatgg tataatgtat
    23701 cttaatattc agttttttaa gatgctagga ggtaattact atgtttaaaa ttaaacactt
    23761 taatgatttg agtttagatg aattttatga aatagcaaaa tcaagatatg aggtatttgc
    23821 ttgtgaacag aagatatttt cattaaacga ttatgatgat atagataaaa gctcttatca
    23881 catattttta aaagaaaatg gattgatttg tgcttacgct agaataattc ctaaagaata
    23941 cagctcttat aatgatgttt ccattggtag agttttagtt ctttctagtc atagaagaaa
    24001 aggattagca aaacaaatga tggattgtgc tatagatttt ataaaagtta acttacacga
    24061 aaataatata accttatcag cacaaactta tataaagaac ctttacttat cttgtggttt
    24121 taaagaaatc tcagaagtat acgacgaagc aggtattgaa catattaaaa tgagattata
    24181 gtgttgacaa gctaatattg ttaatatata atttaagtat aaattaaata ttgaattgat
    24241 atttaggtat ttgaaaatac cttacataga gtcagaaggc ttttgctttc tgactttttt
    24301 attatcaaaa ttcaataatt atatggattc aaagtgttaa tttttattct atattctact
    24361 ttccccaaaa gtaagaatta tacccaactt tgtatgatag gcatcttgcc tatctttttt
    24421 ttgtaaaagt aacaaggtaa taatataatg catagtttaa aataagaaga ttagcccagg
    24481 ctaaatttca agcatggagg tgctattatg aaaaacctta acgatattca agtgaaaaaa
    24541 actgtcaaag ttgaagatat attatctagt ggtaatttaa gagaaagaat gttagcgctt
    24601 ggtcttacta gaggagctgt tatagatgta gttagaaaag gtccaaaaaa taatttaact
    24661 gtctataaaa tcagaggtag caagatagct ctacgacaag aagaaagtag cttaatatta
    24721 gtttctgaca tttaaaaatt tctagcagga ggatattaat atggggttga cacataactc
    24781 aactaaaatg agttctttaa aagacatgtt tgatatagac aataaggaag accagtttgt
    24841 aattgcattg gctggcaatc caaatacagg taaaagtact gtatttaatc atcttacagg
    24901 acttagacag catactggaa actggccagg aaaaactgta gctacagctc gtggtaattt
    24961 taaatataaa aataccgaat atgcattaat agatttacca ggaacatatt ctttatttgc
    25021 cttatcacaa gaagaaattg tagctcgtga ttttatatgt tttggtaatc cagatgcagt
    25081 aatagtagta tgtgatgcta catgtcttga aagaaattta aatttagtat ttcaagtgat
    25141 ggaacttaca gataaagtta ttttgtgtat taatttaata gatgaagcta gaaaaaaagg
    25201 cattactatt gataaaaaac ttttagagga ttctcttggt attccagtta ttttaactgc
    25261 tgcaagaaat ggctctggta tggatgaact attggatact ttaaatgatg tatcttttga
    25321 caaatacaaa ttaaataata aacctgttag atacaatgaa aatatagaga atgtggtaaa
    25381 atcaattcaa ccagaattgg ataatattat accaggtata aattctagat ggttgggact
    25441 tagacttatt gatggcgatg aaagtatatt tgaatctatg agtaattata tagacaaaga
    25501 ttctatatat gctattaatg aagtaaagaa aaagattcca gacaatataa ataaacagaa
    25561 aataagagat gagtttacta aaatcaacta tgattatgct aaaaaactga gtgatgagtg
    25621 ttgttctaat gttgctaaaa aatcaactga tagagaagaa aaagtagata aaatattaac
    25681 atctaaaata tttgggcttc caataatgct cttattatta ggtactatat tgtggattac
    25741 tatagaagga gctaactatc catccacttt attatctaat ttattactcg gatttgagcc
    25801 aagtatttcg ggtatattaa atagtattaa ttgtccatca tggttaaatg atatgttggt
    25861 tttaggatta tacagaacat tagcatgggt aatctctgta atgctacctc ctatggcaat
    25921 attttttcca ttatttaccc tacttgagga ttttggatat ttgccaagag ttgcatttaa
    25981 cttagaccac ctatttaaaa aagcttgtgc acatggaaaa cagtgtttga ctatgtgtat
    26041 gggatttgga tgtaatgcag ctggagttat tggttgtagg ataattgact ctccaaggga
    26101 aagacttata gctatactta caaacaactt tgttccttgt aatggtcgtt ttcctacttt
    26161 gatagctata tctacaatat tttttagctc agttataacc aactctttcg tatcaagcgt
    26221 agcaactgca ctttgcataa ctttactgat aattctaggt gttataataa cattgttggt
    26281 ttcttatact ctttctaaaa cgctattaaa aggagttccg tctacattta cattagaact
    26341 tcctccttat agagttcctc aaataggaag aactttatat acctctataa tagatagaac
    26401 aatatttgtt ttaggaaggg ctgtaatggt agccatacct gctggtgtca taacttggat
    26461 atttgcaaat atatatatag gagatttaag tatattatca catgttgcaa actttttaga
    26521 ccctttagct aaattaattg gtcttgatgg ttttatatta cttgctttta ttcttggatt
    26581 cccagcaaat gagatagttg tacctatact tttaatggct tacctagcaa ctggttctat
    26641 gatagaatta gatagcttta gtgctttagg tcaagttctt agagaacatg gatggactta
    26701 tttgacagct ttaaatgtta tgctgttcag tttactgcat tggccttgtg caacaacact
    26761 tttaacaata aaaaaagaaa ctggtagttt aaaatggact gccttaggat tcttaatgcc
    26821 tactatactt gcttttgttg tttgtttttt aacaactaca gtttacaatt tatttatata
    26881 attagtatta actgacagta gacatatatt agagaaacca tttaaaaatt tatata
//
LOCUS       DAEMIK010000042        26552 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEMIK010000042 DAEMIK010000000
VERSION     DAEMIK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.042"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            19..180
                     /locus_tag="KPK87_001516"
     CDS             19..180
                     /locus_tag="KPK87_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537673.1"
                     /translation="MAIVVIGIYLGLKREFSKLIGFLVVALIAVGLVFNASGVKDVLL
                     ELFNKIIGA"
     gene            240..1013
                     /locus_tag="KPK87_001517"
     CDS             240..1013
                     /locus_tag="KPK87_001517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537674.1"
                     /translation="MKGIKVIMISDEMRVDILHAVFINQNFIKDEENCNYEKYLLELV
                     NKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLEITTRLHASKELTGQIQK
                     FCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFDIQTYVKL
                     LTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAEYDTFLAY
                     IYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            1112..1408
                     /locus_tag="KPK87_001518"
     CDS             1112..1408
                     /locus_tag="KPK87_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537675.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            1327..1830
                     /locus_tag="KPK87_001519"
     CDS             1327..1830
                     /locus_tag="KPK87_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2537676.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            1848..2351
                     /locus_tag="KPK87_001520"
     CDS             1848..2351
                     /locus_tag="KPK87_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2537677.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            2469..2867
                     /locus_tag="KPK87_001521"
     CDS             2469..2867
                     /locus_tag="KPK87_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537678.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            2845..5295
                     /locus_tag="KPK87_001522"
     CDS             2845..5295
                     /locus_tag="KPK87_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2537679.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            5295..7499
                     /locus_tag="KPK87_001523"
     CDS             5295..7499
                     /locus_tag="KPK87_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2537680.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            7496..8503
                     /locus_tag="KPK87_001524"
     CDS             7496..8503
                     /locus_tag="KPK87_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2537681.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYTDLSSSYWQQHLIGAGRVK
                     Q"
     gene            8520..9422
                     /locus_tag="KPK87_001525"
     CDS             8520..9422
                     /locus_tag="KPK87_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537682.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKDSNWKIIG"
     gene            complement(9621..9956)
                     /locus_tag="KPK87_001526"
     CDS             complement(9621..9956)
                     /locus_tag="KPK87_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537683.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKTSVKTIQLVCIEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            9906..10115
                     /locus_tag="KPK87_001527"
     CDS             9906..10115
                     /locus_tag="KPK87_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537684.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRESYEKMLAVSIRAPNLEGM
                     VENFDKDLSHPNETDLIELKNAISK"
     gene            10208..12871
                     /gene="mgtA"
                     /locus_tag="KPK87_001528"
     CDS             10208..12871
                     /gene="mgtA"
                     /locus_tag="KPK87_001528"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBG2537685.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGGIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEEGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEILSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLSSVAKYFYKKKYQELI"
     gene            12887..13390
                     /locus_tag="KPK87_001529"
     CDS             12887..13390
                     /locus_tag="KPK87_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2537686.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWGEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            13528..14205
                     /locus_tag="KPK87_001530"
     CDS             13528..14205
                     /locus_tag="KPK87_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2537687.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            14452..14763
                     /locus_tag="KPK87_001531"
     CDS             14452..14763
                     /locus_tag="KPK87_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG2537688.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            14929..17694
                     /gene="mgtA"
                     /locus_tag="KPK87_001532"
     CDS             14929..17694
                     /gene="mgtA"
                     /locus_tag="KPK87_001532"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBG2537689.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNHRIQFAATNPIKDVLKKLHT
                     TLRGLDVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKVRV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            17707..17874
                     /locus_tag="KPK87_001533"
     CDS             17707..17874
                     /locus_tag="KPK87_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537690.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            18924..19223
                     /locus_tag="KPK87_001534"
     CDS             18924..19223
                     /locus_tag="KPK87_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537691.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEYNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFTVSVSAKRGRSLFALATFAGKGVRL"
     gene            19220..19636
                     /locus_tag="KPK87_001535"
     CDS             19220..19636
                     /locus_tag="KPK87_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2537692.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGVMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            19641..19880
                     /locus_tag="KPK87_001536"
     CDS             19641..19880
                     /locus_tag="KPK87_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2537693.1"
                     /translation="MMKSTKKCLSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVVDMELKGRIRAALMKAILGFEVKVK"
     gene            20198..20341
                     /locus_tag="KPK87_001537"
     CDS             20198..20341
                     /locus_tag="KPK87_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2537694.1"
                     /translation="MNNLIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            20404..22164
                     /locus_tag="KPK87_001538"
     CDS             20404..22164
                     /locus_tag="KPK87_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2537695.1"
                     /translation="MQEVEVIKARNVLSKQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVITKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCLGYDYHPEDKTITVNQEEAEIVRYIFNRYVEGAGGS
                     VIGQELENLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEIFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGSTLSRRNWHSSSEYSKVIWQCVTATKKGKKFCPDSKGIPETAIEEAFIESYRLLC
                     DNNKDVLEEFLHRLDETLSNSTVSKQLSKIEKEISAVEGKKNKLVDMRLEDSIDKDTY
                     ETKYTALMEKLEQLASDRQKLQETAVNEKDIKRRLKEFKTTLEQNEVLDVFDRHVFES
                     IVEKVIVGGYDEDGNKDPAQLTFVYKTGMKNSVDGSRFKPQRKNARGRHRTDELCSHE
                     SNEVNSLCSDNSADTCGNCS"
     gene            complement(22658..23155)
                     /locus_tag="KPK87_001539"
     CDS             complement(22658..23155)
                     /locus_tag="KPK87_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537696.1"
                     /translation="MKKRKIVIITLLLIGVLLLGGILYNLFLVKTANISMLKESWNFD
                     IPIPNKEIEVFDTQDSINGDGQSYFIQEFSEKNFKKVFNLKGGIVVSKDNINEIEKYI
                     DKFKRDSVNINKSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYS
                     LIWNQ"
     gene            complement(23482..23652)
                     /locus_tag="KPK87_001540"
     CDS             complement(23482..23652)
                     /locus_tag="KPK87_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537697.1"
                     /translation="MFRDKMDRCTLTAYIGSSYDYCDFIDTQLDDFILEYGENVVESC
                     LHQVMVLVSKYN"
     regulatory      complement(23706..23802)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            24598..24999
                     /locus_tag="KPK87_001541"
                     /pseudo
     CDS             24598..24999
                     /locus_tag="KPK87_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
     gene            24996..26063
                     /gene="rsgA"
                     /locus_tag="KPK87_001542"
     CDS             24996..26063
                     /gene="rsgA"
                     /locus_tag="KPK87_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG2537698.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQVSKYIKPGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKIE
                     YLAKKEKKFKNISKINKKIRR"
BASE COUNT         8339 a         4443 c         5688 g         8082 t
ORIGIN      
        1 gcacagagct tagtgcttat ggcgattgtg gttatcggta tctacttagg gctcaagcgt
       61 gagttttcaa aacttattgg cttcttggta gttgccctta ttgctgtcgg cttagtattc
      121 aacgctagcg gtgtgaaaga tgtactgtta gagctgttca ataagattat tggagcataa
      181 cgaattttat tgttgctgta atatgagaat gttataatgt gggtacgata aattattttt
      241 tgaaaggaat aaaagtgatt atgatttcag atgaaatgag agtggatatt ttacatgcag
      301 tattcataaa ccaaaatttt attaaagatg aagaaaattg taattatgaa aaatacttgt
      361 tggaattagt taataaatca atatatttta gagaaaaatc aaattttgca gaatatgtac
      421 cacctaaatc tgaaaatcat ggagaatgtg attgtaactc acctaattat caaatggact
      481 ttaaattact tgaaatcaca acaagacttc atgcttcaaa agaacttact ggtcaaatac
      541 agaaattctg tgatggtgta attggtaagt gtccaccgag aagaccaaat acacaaatga
      601 ctgtaactag gttatttgct tcgttgcggg attatgattg tgaaagttta cattcttgtc
      661 ttacagagaa atatgagtat ggaacgattg aatttgatat acaaacctat gtgaaattgc
      721 ttactttcaa gaaaaactta tttttctttt ttccatacaa gtttagtttt aatacttgtt
      781 ataactttaa atatgcttta gacagtataa gaattgcttt agaaaaagat tttagagagt
      841 caaatctttt tagagaaaaa tactatgcag agtatgatac tttcttagca tatatctatg
      901 aagataatct gataataagt aaatttgaac aagacggcaa gttacaaatg attgattgta
      961 tatatttatt taaaagtcaa acatacagta aactttacga gtacacatgg taaatcagtt
     1021 acacttaaac aaattcatac ttacacacaa agcagttagt cttaggattg actgcttttt
     1081 tcttacctaa atttcagatt ggagtgatga aatgaacgat atttttaaag atatgcaggc
     1141 aaaagtcggc tgtgaataca tttccgacct gccctcttac aagcgtaagg tgtggcatga
     1201 aatgaaacgg ctgaaccctg ccgactatga agaaaaacag ttggaagatt tttctaaata
     1261 tgtgtttggt atgtcatacc agaccttaaa agatgtgatg aaacaacaga aaggacgtga
     1321 ggaacaatgc aggaaacaag ggtactggtg gaaacgggaa gaacaaatgg cgaagaaaca
     1381 acatcatact ggtttgactt accgatagat gttgcagagt ttgaagaaaa gttaggtgtc
     1441 ggtgcagaaa gtcaagatta ccgcattatc gaaaaggtgt tgccttatgc tgatgaagtc
     1501 cacgaacaca ccagcgtgta ccagcttaac gaattagatt ttatgtaccg ccagctatca
     1561 gccgatatgc aggaagaata tgtagaactt cttaccgtgt ttgagaactt agaagcactt
     1621 tatatttgca ggaacgtgat taccgtttat cacgactgta aaagcatgat agatgttgca
     1681 aggcaaaagc tgatgaacga cccgacattc aaacatttat ccgaggactg tcaagcatac
     1741 tatttcgatt ttgaagccta cgcttctcac ttacttgaaa acaaacgcta tcttgtaacg
     1801 gaaaacagta tctttgaact gccagaatag gaaatgaggt ggtgcttatg attgatgata
     1861 tggcggttta tattgctaat cttggcagat acaatgaggg atatttagtc ggtgcgtggt
     1921 tcactttccc tattgacgag gaagatgtaa aagaaaaaat cggcttgaat gaacagtacg
     1981 aagaatacgc tgtccatgat accgacaact ttcctatcga cataggggaa tatgtttcca
     2041 ttgaagaact caatgaaatg tatgaaatga tagaggaact tcccgactat atcgtagagt
     2101 gtctggacga atttatcagc cactatggga cgctggaaga agtcgtggaa cataaggacg
     2161 atatttattt ttatcccgat tgtgaaacca tgacagatgt tgcctactac tacatagacg
     2221 aattgaacgc acttggggac attccagcta acttacagaa ctatattgac tatgaagcct
     2281 acgggcgaga tttggatatg ggtggtcgat tcattgaaac aagccgaggt atctgtgaga
     2341 taccatatta acgctggaag atcagcaaca agcattgttg ctactgacag cgtatttctc
     2401 tttaagggac agtctgaaaa tggctgtcct tttctctttg aagaacttac gaaaggggct
     2461 gaaagaactt gaaaaagatt aaaagctaca cgggtatctg gaacgtggaa aaagtcttat
     2521 atgcaatcaa tgactttaac ttaccctttc ccgttacctt tacacagatt acatggtttg
     2581 tgattacgga atttatcatc attctgtttg gggatatgcc cccactttcc atgattgagg
     2641 gagcatttct caaatacttc ggtattcccg ttgctctcac ttggtttatg tcgcagaaaa
     2701 cctttgacgg aaagaagccg tacagctttt tgaaatcaca gataacctat gccttgcgac
     2761 ctaaaatcac ttatgcagga aaagccgtaa aactgcataa gcagatattg aatgaaacaa
     2821 tcacggcagt aaggagtgtg aactatgttc ccgataaaat atattgacaa taaccttgtc
     2881 tggaacaagg acaatgaggt gttcgcctac tatgagctga taccgtacaa ctattctttt
     2941 ctatccgcag agcagaaatt tatcgtgcat gacagttttc gacagctaat cgcacagtcc
     3001 cgtgagggaa aaattcatgc gttgcagatt gccacagaaa gctccattag aagtatgcag
     3061 gagcagtcaa agaaactggt aacgggcaaa cttcgtgagg tggcagtcca aaaaatagac
     3121 gaacagaccg aagcgttagt atcaatgatt ggggacaatc aagtggacta ccgctttttt
     3181 cttggcttta agctcatggt tacggaagaa cagctcaatc tgaagaacat caaaaaatcg
     3241 gcatggctga cgttcaagga atttcttcat gaagtgaacc acacgctgat gaatgatttt
     3301 gtttccatgc cgaatgatga aatcaaccgt tacatgaaaa tggaaaagtt actggaaaat
     3361 aaaatctccc gtcgctttaa ggtgcgtcgc ttggaaatcc atgattttgg gtatctcatg
     3421 gaacatcttt acggcaggga cggtatcgcc tatgaagatt atgagtacca gctaccaaag
     3481 aagaaattga agaaagaaac gctgataaaa tactacgacc ttatccgtcc gacaaggtgt
     3541 gtgattgagg aaagccagcg gtatttacga ttggaacatg aggacaagga aagctatgtg
     3601 tcctatttta ccgtcaatgt aattgtcggg gagttggatt ttccgtcctc tgaaatcttc
     3661 tatttccagc aacagcaatt cacattcccc gttgatactt ctatgaatgt agaaatcgtg
     3721 gagaaccgta aggcattaac gacagtccgc aacaagaaaa aggaattgaa agatttggat
     3781 aatcacgcct atcaagcagg aagtgaaacc agctcaaatg tggtggacgc tttagacagc
     3841 gtggacgagc tggaaaccga tttagaccag agcaaagaaa gtatgtataa gttaagctac
     3901 gtcatacggg tgtctgcacc cgatcttgac gagctgaaac gccgttgtga tgaagtcaag
     3961 gacttttacg acgacctcaa tgtaaaactg gtgcgtcctg ctggcgatat gttgggactt
     4021 cattctgaat ttttacctgc cagcaagcga tatatcaatg actatgtgca gtatgtaaag
     4081 tcagacttct tggctgggct tggttttggt gcgacccagc agttagggga aaacacgggt
     4141 atctatatgg gctattccgt tgatacggga agaaatgtgt acctgcaacc gtctttagct
     4201 tcgcagggag taaaaggcac agttactaat gcccttgctt ctgcttttgt gggaagtctt
     4261 ggcggtggaa agtcattctg caacaatcta cttgtatatt atgcggtgct gtttggcggt
     4321 caagcacttc tgttagaccc taaaagtgag cgtggcaact ggaaagaaac gctcccagag
     4381 atagcccatg aaatcaatat cgtaaacctt accagcgaca aggacaatgc aggacttctt
     4441 gacccatttg tgattatgaa gaatgtgaaa gacgctgaaa gtctggcaat cgacatctta
     4501 acattcctta cgggtatttc ctctagggac ggcgaaaaat tccccgtgtt acgaaaagcg
     4561 gtgcgttctg ttacccagag tgaaaataga ggactgttac acgttattga ggaattgaga
     4621 aaagaagata ccgctatctc acgcaatatc gcagaccata tcgacagctt cacggactac
     4681 gactttgcac acctgctgtt ttcagacggg acggtagaaa atgctataag ccttgataac
     4741 cagctcaata tcattcaagt agcagacctt gtactgccag ataaagatac cacttttgag
     4801 gaatacacga ccattgaatt attgtcggtg tctatgctga ttgtgattag tacctttgcc
     4861 cttgatttta tccattcgga cagaagcatt tttaagattg tagatttgga cgaagcgtgg
     4921 gcgttcttaa atgtggcaca aggcgaaacc ctatcaaata agctggttcg tgctggacga
     4981 gctatgcagg caggcgttta tttcgttacc cagtctgcct atgacgtgtc aaaggaaagt
     5041 ctgaaaaaca atatcggttt gaagtttgca tttcgttcta ctgacatcaa cgagataaag
     5101 cagaccttag aatttttcgg tatcgacaag gacgacgaaa acaaccagaa acgcttgcgt
     5161 gatttggaga acggacaatg tttattgcag gacttatacg ggcgtgtcgg tgtggtgcag
     5221 atacacccag tctttgaaga attactacac gcctttgata ctagaccgcc cgtacagaga
     5281 aatgaggtgg agtgatgaaa gaaaggatac agagtgcgtt cacaaaaagg aagatacttc
     5341 actttctcaa aatggctctg ttcgtggtgg cactttccct tatcctgctt tcacttctgg
     5401 ggacggtggc tcatgcgacg gggcttgtgg acgataccat aaacgcagaa aatctatatt
     5461 caaaatatcc gctttccaac taccagcttg attttttcgt cgataatagc tggtcgtggc
     5521 ttccgtggaa ctggctggac ggtatcggaa aatcggtaca gtacgggctt tactgcatta
     5581 ccaactttgt ctggacgata agcctttatt taagcaatgc aacgggctat gtggtgcagg
     5641 aagcctataa acttgatttt attaacgata tggcagacag tatcggaaaa agcatacaga
     5701 cccttgcagg cgttactgag aacggctttt cttctacggg cttctatgtt ggtttcctgc
     5761 ttcttattat cttagtggtg ggaatgtatg ttgcctatac gggactgata aaacgggaaa
     5821 ccagcaaggc acttcacgct gttatcaact ttgtggtggt gttcgtgctg tccgcttcgt
     5881 ttattgccta cgctcccgac tacatcaaga agataaatga attttcatca gacatcagta
     5941 ccgcttcgct tgatttggga acaaaaatca tgctccccaa ctctgacagc gagggcaagg
     6001 acagcgtaga cttgatacgg gacagcctat tttctattca agtacaacag ccgtggctac
     6061 tcttacagtt tggaaacagc aacgcagagg aaatcgggac agaccgtgtc gaagctctcg
     6121 tatcggcaag tccagaggac gaggacggaa agaccagaga agaagtcgtg aaaacagaaa
     6181 tcgaggacaa cgacaacaac aatctgacga taccgcaggt ggtaaaccgt ttaggtatgg
     6241 tgttcttcct cttgttcttt aatcttggta ttacgatatt tgtattcttg cttacgggta
     6301 tgatgttgtt cagccagata ctttttatta tcttcgctat gtttttacct atcagctttc
     6361 tactttccat gataccgagc tatgaaagca tggcaaagca ggcaatcgtg agggtattta
     6421 ataccataat gacacgggca ggaataacgc tcattgtaac ggttgcgttt agcatttcca
     6481 gtatgtttta taacatatcc acggactatc cgtttttcat ggtggcgttc ttgcagatag
     6541 tatgtttcgc tggtatctat atgaagctgg gcgacttaat gagtatgttt tctctcaacg
     6601 ctggcgacag tcaaagcatg ggacgcagga tattccgcag accgtatctg ttcatgcgac
     6661 atcgggctag gcgtatggaa caccgtattg caagggctgt aagtgctggc ggtatttcgg
     6721 gcggtgtggc tggtgcggta gctggaagtg ccgttgctgg caagagagcc gaaagaaaaa
     6781 atacggcttc taaagaaaat cggggcaata ccacttccag tatgggacag cgtgcaggct
     6841 caaaggtggg tgctgtctta gatacgaaaa ataaagtgaa agacaaggca aacgctgtca
     6901 aagagaatat caaggatatg cccacacaga ccgcttatgc ggtgtattcc gcaaaggaaa
     6961 aggcaaagtc cagcgtgtct gatttcaagc gtggtatggt gcaggaacag cagtccagac
     7021 agacgggacg cttggaaaag caggaacagc ataagaaaag tatcgctgtc aagcgtatgg
     7081 agcttcaaaa ggcacaagaa gcaaggcagg cacagcgaaa ggctgacgga tcagcgacaa
     7141 cgggagctac ccgtccccat gagcgaccag ccacagcttc aaccgtttca aagacgggtg
     7201 cggaaaaaat gcaggaagtc aaacgccctg ccacaactcc gactacgaaa gcaagcgaac
     7261 cagttaagac aagcggtgtc aaagaacgtc cgctatcttc tagtactcct gacgggaaag
     7321 cgacccaatc gacatatagg cagaatgtag aaaaagttac cgtgtcaaag gaaacacgcc
     7381 agaattacac caaagaccgt aggacaaagg ttcagcagac ccagactgtc cagaagaacc
     7441 agcagaccac agagaaaatc cgtaatcttg taacgaaaaa aggacagaag aaaaaatgaa
     7501 actgaaacat atcgctatca ttggcagtct gtttcctatt ctcttttctc tggtgctttt
     7561 ctttggggtg ctgattagtg cggacagcga cgacgaaaac agcaactttt cttctggcat
     7621 tacgggtatg aacttatccg cagaagtttt gaaacatcag cctatggtag aaaaatacgc
     7681 cagagaaaac ggtatctccg agtacgtcaa tgtactactg gctatcattc aagtagaaag
     7741 tggcggtacg gcagaagatg ttatgcagag ttcagaaagt cttggtttac cgcctagttc
     7801 cttagatacg gaaagctcaa tcaagcaggg gtgtaagtat tttgcgtccc tgctttcttc
     7861 ctgcaaaaat caaggtatcg acgatttgaa tgtagcgata cagtcctata actatggcgg
     7921 tggctatgtg ggctatgtgg caggaaacgg aaagaaacac acttttaatc ttgcagagag
     7981 cttcgcccgt gagaaatcgg gtggaaagaa agtaacctac accaacccga tagccgttgc
     8041 gaagaacgga ggctggcggt atggctatgg aaatatgttc tatgtggaat tagtcaatca
     8101 gtatttaact gttccgcagg tatcgggaga gctggcacaa aaggtaatga atgaagcgtt
     8161 gaaatatcaa gggtggaaat atgtgtttgg tggttcttct ccaaccactt cctttgactg
     8221 ttcgggactg acgcaatggt gctatggaaa agctggtatc tccttaccga gaacggcaca
     8281 agcccagtat gacgctaccc aacatcttcc actctcgcag gcaaaggctg gcgatttggt
     8341 atttttccat tccacctata acgctggttc gtatgtaacc cacgtcggta tctatgtggg
     8401 aaataatcag atgtaccatg caggcgaccc gataggatat acagacctaa gtagtagtta
     8461 ctggcaacag cacttaatcg gtgcaggacg agtaaaacaa tagaaaggac ttgaaaaata
     8521 tgtttaagaa gaataagaaa cagacagaaa atgtcaaaga aaaaaaggta cgtactgtca
     8581 aggtaggcac acataagaaa accgtgattg cattgtgggt ggtgcttatt gcaagcgtga
     8641 gctttggagt atataagaat tttaccgcta ttgaccagca cacgacccat gaaaaagaag
     8701 tgattgagct tcgcttgcag gacaccaacg ggatagaaaa tttcgtgaag aattttgcaa
     8761 agtcctatta cacatggagt aacagcaaag aagcgattga cgcaaggacg caggcaatca
     8821 gcggttatct gacaaaggaa ttgcaggact tgaatatgga taccattaga acggatatac
     8881 cgaccagctc tgccattaca gatgtgattg tatggagcat tgaacagtct ggaacggaca
     8941 ctttttccgc tacctacgaa gtagatcagc agataaaaga gggggaacag acaacagcgg
     9001 ttaaggcaac ctataccgta aaagttcatg tggacgctgg cgggaatatg gtaattattc
     9061 agaaccctac ccttgcacca gcaatcgaga aatcagacta tgaaccaaag acaccagaag
     9121 cagacgcaag tgtagacgcg gataccataa atgacgctac tgcatttctg gaaactttct
     9181 ttaagctata cccaacagcc acagaaaagg agcttgctta ctatgtgtct ggaaatgtgc
     9241 ttgagtctat cggcagggac tacctctatt ctgaattggt aaaccctatc tttacggttg
     9301 atggggacaa tgtgaaagtc aaggttgccg tgaaattcat tgataatcag acaaaggcaa
     9361 cacaggtatc gcagtttgaa ttagtgctac ataaggatag caattggaag attataggat
     9421 aattattgaa agcgtgcatt tctttaggaa tttgcacgct tttgttaaga gcattattct
     9481 ttatatttat gaaagagatc ttaaacaaag tattaatggc ttcgcaaaat ttttttctta
     9541 atatatttgg attagaaaaa gcgcatctta attgtagact tactatacat atgaattatg
     9601 ccagtcaacg tatttaaaat ttatgttgtt ttattttgtt gtaaatcgca agaatagata
     9661 agagtaatca atggtgaagg ggaattatta aaaatactat tggtttcttc tgtcccgttc
     9721 caatttgcgc attgtatttt ttcataaatt acagagtggt caattatgta gttcagatat
     9781 tcaatacaaa ctaattgtat tgtttttaca ctcgttttcc atactgccac ttttgtcatt
     9841 ctatcactca taatacaaat attattatga gcaacttttc taagtagttc atttaaattt
     9901 gatacatggt caaggtgaaa accaagaaga agtttgtcag cactcatatg tgtcaagggg
     9961 aaatgcctat ttatgtgcga gagagctatg agaaaatgct tgccgtatcc atccgtgctc
    10021 caaatttaga aggaatggtt gaaaactttg ataaggattt atctcaccct aacgagacgg
    10081 atttgatcga gctgaaaaat gccatatcaa agtaaattgc aagaaaaaag caagaatata
    10141 aatatagcat tttcagaaca ctgtgttaaa atgttattac ttatacttat aaaggagggg
    10201 atgtttcgtg tcaaaagcaa cactatttga tagtcgtatc aaaaaatacg catactgtag
    10261 aacatcagaa atttatcgag acattggctc atctccggat ggtttatcca ttgagcaaat
    10321 cggttcaatg cgggagaaat acggagcgaa cagcttcaat gggcggaaaa atgatacgac
    10381 gatgcaacgg ttgagacggg catttattaa cccgtttcat gtgattcttt ttgttctggg
    10441 cattgtttct cttgttacgg atgttttcgt agcatccaat tttgcgagaa atgctactac
    10501 cgccattatc atcttttcca tgatcgtaat tagtggcgga attcgtctga ttcaggagct
    10561 gcgagctaag agcgctgctg ctcagcttga tcgcctagtt catgaaagcg tcacagtgag
    10621 gcgtgatgga aagttaatag agattcctgg cgaagaatta gtggtagggg atattgtact
    10681 attctctgca ggtgatcgtg ttccggcgga tatcagattg acgaagatta cggatctctt
    10741 tatctctcag gctgcgatta ctggcgagag cgccatcatt gaaaagagct gccgaaaact
    10801 cagttacgga gagcaggata cactgactca gcttgaaaac ctcgctttta tggcaaccac
    10861 agtcatcagt ggaaaagggg aaggcattgt actggcagta ggcaaagata ccctgtatgg
    10921 aggatttacc aaaccggatt ctgatgataa aaattccttt aaaaaaggag ccgattccat
    10981 cgcctgggtg atgatacgtt ttatggcgat attagttccc attgtattta tattactggg
    11041 gattactgat ggaaaatgga tggaatcctt tgctttcgct ctgtcagttg ctgtaggtct
    11101 gatgccggaa atgcttccta tggttattac agcctgcctt gccaaaggaa gtcttgctat
    11161 gagcaaaaag cagaccatta tcaaggatat taatgccatg cagagctttg gtagcatgga
    11221 tgtgctttgt atggataaaa caggaacact taccaatgaa agcatcctgc tggaatatta
    11281 tatggatatt tttggaaatg aaaatgcaga ggttcttgat cttgcttatc tgaatagctg
    11341 ctatcattct ggtgtctgca ataccattga taatgccatt cttgcctgca ataccatgcc
    11401 agggcgggaa gcccattacg caaatcttct gacaaaattc caaaaagctg atgaaattcc
    11461 ctttgactat gctcgtaagt ttgtcagtac acttgtacag gatagcaatg ggaatagtca
    11521 gcttattatg aaaggtgata ttgaacatat attctcacgc tgcaaccatg tagaatatcg
    11581 tggaaaactt ctccccatgg ggcaggatgc gagacagagt gtatcttctg tagtaggtga
    11641 aatgttacag gacggaatga aggtcattgc ggtggcacga aaaaatgtgg gagcgcaaaa
    11701 gaaaataact cctgatgacg aatggggcat gactctagtt ggctatctat ccttctttga
    11761 tgcaccaaag cagacagcaa aggagtctgt aactgcactc aaaagattaa aagtgatccc
    11821 taagattctg acaggggacc aggcagcgat tgctgtgtct gtttgccgcc gtgtgggtat
    11881 ttatacagag cgtatcttaa ctggtactca actggatgaa atgacagact gtgaacttaa
    11941 aaaggctgtg gaacatactc atgtttttgc agagcttact ccgggacata aggtgcgtct
    12001 tgtctctgct atgcaggata atggtcattc cgtgggattt cttggtgatg gagtcaacga
    12061 cattccggca ctgaatgaag caaatgttgg aatctctgta gatacggcag tggacgcagc
    12121 gaaagatgct gccgatgtgg ttttgctgca aaaggacttg aatgttttgg aggaaggtgt
    12181 aatggagggg cgaaaaactt ttaccaatat gctgaaatat attaaaatca ccgctagctc
    12241 taactttgga aatatttttt ccatcatttg tgccagtgcc tttttaccgt tcctgcctat
    12301 gacttcaatt cagattctgc ttttgaatct gctgtatgat acgttgtgta ttgtgcttcc
    12361 ttgggacaat gtagatgagg aagagatact gtccccaaga gattggtccg gcaaaacact
    12421 gagacagttt atgctgtctt tcggaccgat cagttctctg tttgatattg tcaccttcct
    12481 atttttgtat tatttcctat gtccgatgct atgtggaggg gcaacttatc tgcagcttac
    12541 agatccttca ctgcaacttc agtatgtagc actgtttcag acgggatggt ttttggaatc
    12601 tatgtggaca caggttttga ttttacattt tctgcgaact tccaagattc cttttttcca
    12661 gagcagggca tctgcaccgg tcatttctat aactctagtg gggattattg cttttacttc
    12721 ccttacattt gtcggcggtg cgtcgttgtt tggactgacg aaattacctc tttggtattt
    12781 cgtattttta ctgctggtgg tatctctcta tatgctgctg agttcagtag ccaaatattt
    12841 ttataagaaa aaatatcaag agttgattta agaggaggca ttcatgatga aaaagaatat
    12901 tagttatatt ccgatggatt cggctcttga aatctggcta ttagaaaaac tactaagaga
    12961 tccaccggac agtgattgtg cgaaggattt actttctggt attcaggaga tgattcgtgg
    13021 agaggtatct tcgttattgt taggagcatc agaagaaagc gattggccag tgggtactct
    13081 gtcagataga atcctggcag gggatctgga aatttatcca agaggcagaa aagtactgat
    13141 aaaaggttct gaagtcaacc tgactccaaa ggaatttgat attttgtact ttcttgtcca
    13201 gaacaaaggt gaagttttta caaaggaaca gatttatcgt gccgtatggg gggaagatta
    13261 tattctggat aacagcaaca ttatggcttt tatccggaag ttgcgaaaaa agattgaacc
    13321 tgctccggat tgtcccacat acattttgac catctggggt attggataca aatttaatga
    13381 tcagatatga caaagacgga ctaagaccat tttcggtttc agtccgtcta ttttcttagc
    13441 aaatcgcaag aaaatcgcaa gactttagcc ataggattgt atttgaattt tttatatact
    13501 gttagtagtt aaagggaggt gtttcatatg ttttacacag attttatttt gagaattgcc
    13561 ctctccttaa cacttggatt tcttattgga ttagaacggc aattgactgg tcatcctgca
    13621 ggtattcgta tcaatgtgtt aatctgcatg ggtacaagtt tctttacctt gtttcctatg
    13681 ctctacggtt cggatcaggt attccgtgtc ggaagcagta ttatatccgg tgttggtttt
    13741 ttatgcagtg gtgttatttt caaggacaga gggacagtac gtggaatgaa taccgctgcc
    13801 accttatggt gtactgcagc cattggaatt cttgcaagta cgggaatgta tgcaatggcg
    13861 atcaccgccg ctactatttt aattggttcc aacctgattc tacgtccctt tgcaagaaag
    13921 tttcacccaa ttacttccag tgatgaatct gaaaagcagt atcgtatttc ggttacatgt
    13981 caggaaaagg cagagcagga tatacgctta ttgctgatta acagcaattc ctgtaagacg
    14041 ctctttttga acaatctgga aagcggtgat gtggttggtg acaaggttga gattgtcgca
    14101 gaatactgtt ctgtcggaaa atccaaaaac aatattttag aaggaatcgt tgggcaggta
    14161 ttggtaattc cggaagtgat tagtgctggt tgggaggtgt tgtgagtgag gagagaaatc
    14221 gaagatttga aacgagcaat tctttttgct ttctaccctg tatttacaat cttatgtagc
    14281 tatttgggtt ggaatttctt tgaacaaaga atggcaatct ttaagatatg gtatcaggtg
    14341 agtattgtga tctggtttat tcttgctgtt actattattg tgctcttcat taaacttctg
    14401 aagctgacga aactaagaaa caggtttaag cactgaaagg aggaacaaac gatggatttt
    14461 acatgtatct tttttggaat tttatttact cttgcaggct tttggctggc ctttggaaaa
    14521 ggatatcgtc atctttccct gtggaaaaat atgccgcagg aggagaaaga taaaattaat
    14581 attgtcccgc tttgtcgtaa tgttggagaa atgattgcgt taaatgggat tatattctta
    14641 atgaaaggat ttttgccggg attttcaaac cactggtttg tatttgcaat aatcgcatgg
    14701 ctgattgttg ccggctttga tgtctggtat atcgaaaaaa gtgtccgcta tcgtaaccaa
    14761 taatccacat tgaggtttca catagatatt tttttaagat tgtgaggtga aagacatgga
    14821 ctctgatgtc agtccacgaa ggcagagaat attatatgag aagttggaga ttccgtgtcg
    14881 cccatataat attccttgcc cctatgaaaa ttcaaggagg taagaccaat gaacaaaaaa
    14941 gaaaatcgta tggctattcg tcagactgta gaaaaagcag tcatccgtga cgaacagaac
    15001 caccgcattc aatttgcggc aaccaatcct attaaagacg ttttaaaaaa acttcatacc
    15061 acacttcgtg gcttagacgt agatgaagta tccgtaagtc gtgctaaata tggtaccaat
    15121 aaagtcactc atgaaaaaaa gaaatctctg gcaaaacgac tggttggcgc atttatcaat
    15181 ccatttaccg ccattttatt ttgtctggct ttagtatcca caattacaga tatgattttt
    15241 ccgtattttt ctctgtttgg cagtgtgcca gaggactttg attccctgac tgttgtaatc
    15301 attttaacaa tggtattcat ttccggtacg cttcgttttg tacaggaatc cagaagcgga
    15361 aatgcagcag agaaattgtt agccatgatt acgaccactt gtacagttac tcgtagaaac
    15421 tatgaaaaaa aagaacttcc tatggatgat ttggtggtgg gagatattgt gcatctttct
    15481 gctggagata tgattccggc ggatgttcgt attttggatg cgaaggattt gtttgtcagt
    15541 caggcaagtc ttactggtga gagtgaacct attgaaaaaa cacccaatgt aagtgctcag
    15601 aaagaaagcg tgactgatta cacaaacatt gcttttatgg ggagtaatgt aatttctggt
    15661 agcgcaacag ctgttgttgt ttgtgttggc gatcatactc tgtttggctc tatggcgtcc
    15721 gcagttgctg gagaagcggt tgaaacaagc tttaccaagg gtgtcaatgc cgtttcttgg
    15781 gtattgattc gttttatgct cattatggtt ccgctggtgt tctttgtcaa tggtatcacc
    15841 aagggcgatt ggttggcggc cttcttattc ggcgtttcta ttgctgttgg tttgacacca
    15901 gaaatgttgc cgatgattgt aacaacctgt cttgcgaaag gcgctgtttc catgagcaag
    15961 aagcagacaa tcgttaaaaa cctcaattcc attcagaact tcggtgctat tgatattctc
    16021 tgtactgaca agactggcac gctgacccag gataaggttg tgctggaata ccatctgaat
    16081 gtaaatggcg aggacgatac gcgagtactg cgtcacgctt acctcaatag ttacttccag
    16141 actggctata aaaacctgat ggatttggca attatccata agacggagga agaggaagcc
    16201 gcagattcgc gtctcattga cctgtctgaa aactatgtga aagtagatga gattcctttt
    16261 gacttcacac gtcgtcgtct gactacagtg gtacaggata ataagggtaa gacccagatg
    16321 gtaacaaaag gtgctgtgga agaaatgctc tctatttgtg cgtttgcaga gtgtaatgga
    16381 ggagtgcagc ctctgactga tgacgttcgt tacagaattc tggagacagt ggacaatctt
    16441 aacgataagg gattccgcgt actagctatt gcacagaaga gtaatccttc cccaatcggc
    16501 gcatttggtg ttaaagatga gtgtgatatg gtactgattg gatatctggc attccttgat
    16561 cctccaaaag aatctactgc ggatgctatt aaagctctga aagaccatgg tgttataacc
    16621 aaaattttga caggtgacaa cgacaaagtg actcgaacta tctgcaagca ggtgggatta
    16681 gaggttcgca atatgcttct cggttctgat ttggaacaaa tgggcgatgc agagcttgca
    16741 agagtggcag agtctacaga tgtatttgca aagctaaccc ctgatcagaa agtccgtgtg
    16801 gtttctgttc tccgtgaaaa tggtcatacg gtcggtttca tgggtgacgg tattaatgat
    16861 gctgcagcta tgaaagccgc cgatattggt atttcggtgg ataccgccgt ggacgtggca
    16921 aaagaatctg ctgacattat tctattggaa aaagatctga tggttttgga acagggtatt
    16981 attgagggac gaaagacata tgccaacatg atcaagtaca ttaaaatgac tgcttcatcc
    17041 aacttcggca atatgttctc agtgcttgct gcatctgctt tgttaccttt cttaccaatg
    17101 atgagtgttc atcttatatt tctaaactta atttatgatt tatcctgtac tgcaattcct
    17161 tgggataatg tggatgagga gtttattacc aaacctcgta aatgggacgc ttccagtgtt
    17221 ggaagtttta tgatctggat tggaccaacc agttctatct ttgattttac aacctatatt
    17281 tttatgtact ttgtattttg tccattgttc gtatcgaatg gtgtattatt taatgatttg
    17341 cctgctcatt tcagcggtgc agaactggca atgcttcagg cacagtatat cgggatgttc
    17401 caagcagggt ggtttgtgga atctatgtgg agccagacat tagtcattca catgattcgt
    17461 actccaaagc tgccttttgt tcagagtcgt gcatcagctc cggtgacttt gctaacaatg
    17521 actggaatta tagttctgac cattattccg tttactgtgt ttggaactat gcttggattt
    17581 gttgcattgc ctgcagcata ctttgcttat ctgattccat gtattctgct gtatatgact
    17641 ttggcaacaa gtttaaaaaa agcctatgtc cgtcatttcg gagaactgct ttaaggaggt
    17701 ggaatgatga actggaaaga actttggatg accctttttg gaactaccga atggcttggt
    17761 cttaatatcg gcttttgggt ggctatggca gttgtgttgc tgattgttat tgtaatgaat
    17821 gtagtgtttt ggtctatgaa accaaaaaaa gacacaaaag agtcttataa ataagatatc
    17881 agtatgaatg aatcaaggga gatgcacagt ttttattgtg gaatctccct atcatatcaa
    17941 ggcgcattca attgtggcaa tttagtagta aagtatgtgt ttttttatca ttgaattacc
    18001 tataaatgct gtatataagt ggataattgt aatttcgata taaaaattat ggtacttaaa
    18061 agaaatttcg ttttttattg tttgtttacg cgtctataac gaaatcgtga aataatattc
    18121 aaaggtagat tttattatgg aatgtatgtc tgtagaagaa gcttcgataa aatgaggaga
    18181 tctctgaaag aaggatataa aaattatgta atggaaacag aatcccaaat ctattgtaac
    18241 aacaatgaca ggtaatgaat ggttacgtag attgataatt cttattcaaa ttattatcta
    18301 tctaatattg agtggtgttt tgtaattaca tttttataca aaaagaatat gttgaacgca
    18361 caatgataaa tattttgtat tccccaacta atagaataaa gtttttgctt ggaaagtatg
    18421 ttatataggc attatggttt gtaatgatca cgttgacaga ttataccgct atatatgaag
    18481 tcaaagaact taaatcattt acaagtgaga taatctatct aagtctaaaa aactggattt
    18541 ctcaatctat tatcaatgag tccattactt attatttgtg ttttgcaaag aaatgttttc
    18601 tactctagtt tactttttag ttactaaata tcgggaattg gttttgttgg actatattga
    18661 tctgaaagta tacaaaatat tatttcgtgg tctgctgtaa cccctataag tttattagat
    18721 gcacaaggat tgctacctta tatttcaatt ctactttgtt attttgcaag actgattata
    18781 aatacatacg gctttagagg ggttagttta gttctttttg aacttttgaa taagacgacg
    18841 gttgtatagc cgccgtttaa tttttgccta acgttggaca aattactgga tggtcttgtt
    18901 ttatcggctc taggggtagc cgtatgaaac acttacatat tcaacaactt tcgaccatcg
    18961 gtagttcatt aaaaaaacat ttaaattcta cggaatataa tgctcctata tgtggttttt
    19021 tacagcatat aacaggaata atttgtttag cacaagatca atacgtctgt attgcccttg
    19081 tgctttttta ctgcataaaa ctttttcaaa aaatttttgc ggttgggtat caaatcccac
    19141 ctttcacggt gagtgttagt gcgaaaagag gtagaagtct tttcgcttta gcaactttcg
    19201 ctggaaaggg ggtgagatta tgaaaccatc ttctttcgag ggcgctatca gacttcaatt
    19261 tgattgtctg gctcgcaagg ttgtaggacg aactgtaaag aactatgacc gagagcttgg
    19321 cagacgtgca agacgtgaaa cgccgttctg tgatttatct gaaatggagt taaaccagat
    19381 cggtgtcatg gacgagtatt gcgttgagtt tacgtcattt gatgtatttg gttcagaagt
    19441 ccatatctat gatgaacgct tatgtgaagc tatcaaagaa ttaacagaaa gccgacgtaa
    19501 cattctgctt atgtcctact tccttgaaat gaccgacgct gaaattgcag aggttatgga
    19561 aatggaacga ttttccgtat gcagaaatag gctgcacaca ttaaagctca tcaaggatat
    19621 gtatgaggag gaataaacac atgatgaagt ctacaaaaaa gtgtctgtca ttttcgacca
    19681 tcagctctgc ggctgatggt aacgaggtgg caatcgaaaa gattttgaac cattatgacg
    19741 cttacatatc aaaggccagc ctgcgcccgt tctatgatga acacgggaat atgtatatcg
    19801 tggttgatat ggaactgaaa ggcaggatac gggcggcatt gatgaaagca attttggggt
    19861 ttgaagtcaa agtgaagtaa atataacgag agtgtctacc acctcatttc aactctttcc
    19921 tgttctttga caagtgaata cagtaatcag atacgtttga tatacgacga gccagcggac
    19981 tggaacgcca cgaccattga aagggggata gcagagagcg ataatgccag tgaaccgcaa
    20041 gtgatctttg gaaaggttgc tgccatgact cgtatatctg gataatgata cacccgtatg
    20101 acacggttca cccatcagaa tgggaatggt gaaagtccag tggagctgcc aaagccgtct
    20161 gattacttcc ataaataaaa aagcaaggag ggttttaatg aataacttga ttaggtatag
    20221 tatgcaaatt gctatgttaa aacaactgct tgcccgtaaa ctgatttctg aaaaggaata
    20281 cactcttgta aaacaaagac tgatgaaaga ttataaagtg ctttctgaca taactgcttg
    20341 aattgtgttt ctttaagtga tataataaca ctataaaagg aacacaaaat aaggaggaat
    20401 attatgcagg aagttgaagt aataaaagca agaaatgttt tgtcaaaaca agcccgtgga
    20461 aaagccattg agcgtttgcg tgtagctgca tattgccgtg taagtacaga tagcgaagat
    20521 cagttaaaca gttataaatc acaagttcag tattatactg atatgataaa gaaaaacaag
    20581 gaatgggtgt tagctgacat ttatgctgat gaagccatta cgggtacaca agtaacaaaa
    20641 cgagaagatt ttcagcgtat gataaatgac tgtatgaatg gtgaaattga catggtaatt
    20701 acaaaatcca tttcaagatt tgccagaaat actttagaca cattgaaata tgttcgtatg
    20761 ttgaaagaga gaaatattgc cgtctatttt gaagatgaaa aaatcaatac acttacaatg
    20821 gatggagaat tattgcttgt tgtccttagt tctgttgcac agcaggaagt cgagaacatt
    20881 tcagccaatg taaaaaaagg gttgaaaatg aaaatgaagc gtggcgagct ggtaggtttt
    20941 caaggttgtc ttggatatga ttatcaccca gaggacaaga cgattactgt aaatcaagaa
    21001 gaagcggaga tcgtcagata tattttcaac cgctatgttg agggtgctgg tggttctgtt
    21061 attggacagg agcttgaaaa tctaggttat aaaacgaagt atgggagttc cacatgggca
    21121 ccatctacgg ttatcggaat tattaagaat gaaaaataca agggtgatat actcttaggt
    21181 aaaactttta ctgttgatcc gatttccaaa cgtcgccttg aaaacatggg tgaagaagat
    21241 aaattttata tgaaagatca ccatgagccg attatcagtg aagaaatttt tgaaaaggca
    21301 caggaaattc ttaatcggag aaataagaac cgtacaacgg taggcagcgg caagcgtgag
    21361 aagtatagca ggaaatacgc atttagctgt atgctggaat gcggcttctg cggcagtacg
    21421 ctatcaagac ggaattggca cagtagttca gaatacagca aggtaatctg gcagtgtgta
    21481 acagctacaa agaaaggcaa gaagttttgt ccagatagta aaggaatacc agaaacagcc
    21541 attgaagaag cgttcattga aagttaccgt ttattgtgcg ataataacaa agatgtatta
    21601 gaggaatttc ttcataggtt ggacgaaaca ttaagcaaca gtaccgtgag taaacagctt
    21661 tctaaaattg agaaagagat cagtgccgtg gagggcaaga aaaacaaact ggtggatatg
    21721 cgtcttgaag atagtataga caaggacacc tacgaaacca aatatactgc actcatggaa
    21781 aaactggaac agcttgccag tgatcgtcag aagctacagg aaacagcagt gaatgaaaaa
    21841 gacatcaaaa ggcgtctgaa agagtttaag accacacttg aacagaacga ggttttagac
    21901 gtgttcgacc gccatgtatt tgaaagtatt gttgaaaaag taattgttgg tggctatgat
    21961 gaagatggga ataaagaccc tgcacagctc acatttgttt ataaaacagg tatgaagaat
    22021 agtgttgatg gcagccgatt taagccgcaa agaaaaaatg caagaggaag acatagaact
    22081 gatgaattgt gttcacatga gagtaacgag gttaattcat tgtgttccga taatagtgcc
    22141 gacacatgtg gaaactgtag ctaagataat caaacaatag taatgtttat atagattata
    22201 tactaataat atattgtatt tgccaccgat ttgccactgt aaagtaattt tggtggcaaa
    22261 tttagtctac aatgttatca aaaatctcta aagttttatt tttaattcta tagaacttat
    22321 tataattgta attttaaaag aaatcgtcaa tctacagttg ccagatttta tacaactcat
    22381 ttttttcata gaataattta cataatctat agttgaatat tatttaatat ttttatatta
    22441 taattcacta gtaaatatag gcattacaga tgttgataaa catgtgaaag cacatatcca
    22501 cttataatta tttttttaaa aaccataaaa tcacctcttc atttgttgta tgttcttata
    22561 acaaatgaag aggtgatttc cagacaattt aaaaggtttt tgtatattga ctaaaatttg
    22621 tttttattat ttataattct taataataca tatgtaccta ctgattccat attaaactgt
    22681 ataatttttg ttcatctttt tttataatta aaactacata gttttcataa tttctcttat
    22741 atatatactt atcatctttt ttaacttcta acttatattt tttaaaatct tcttcaattt
    22801 tatttttatt acttttatta atgttaacag aatctctttt aaatttatca atatattttt
    22861 ctatttcatt aatattatct ttgcttacaa ctataccacc ttttaaatta aaaacttttt
    22921 tgaaattttt ttcagaaaat tcttgaataa aataactttg tccatctcca ttaatactat
    22981 cttgagtatc aaatacttca atttctttgt taggtattgg tatatcaaaa ttccaagatt
    23041 cctttagcat tgaaatatta gctgttttta ctaaaaaaag attatatagg atacctccta
    23101 ataacaaaac acctattaat agtaatgtga ttattacaat ttttcttttt ttcatttttc
    23161 gttcacaacc ttttgaaaaa acatgattta attcatcatt tgaaggtatg tcgtcaagaa
    23221 taattttgtt aatctaatcc ttcaaactta aaaatcattt atgtttacag aatttcattt
    23281 taagagatgt ttattttcac atagataaga gaatttaagt tctcatattt attctttgat
    23341 acaaatttac atctttatta tatttatttt aaataaaaaa taaaattatg aatactagaa
    23401 atctatctta attagctttc taacataaga ctatttaaag tagataaaac taaaaagggt
    23461 tctctagaaa accaaatcta tttaattata cttacttact aataccatca cttgatgcaa
    23521 acaagattct accacatttt ctccgtactc tagtataaaa tcatctagtt gtgtatctat
    23581 aaaatcacaa taatcatatg aactaccaat ataagcagtc agtgtacatc tatccatttt
    23641 atctctaaac atatatatta ttatccccct aaaaaacttt cataaaaaca aaaagaatat
    23701 ctctttttgg caactggctg acataactca tagtttattt tataagcgtt agtccctata
    23761 gctttgcgtc actaaatttc tctagttttg ccgatttagt tttattctac aattacaata
    23821 atacaataga attggttatt attcaatata cttatttgaa aattaaatga atttataaat
    23881 atgaacgtta ttttatatca aaaaaatagg ctatgtatgt agaatgtatg gaaaaacttg
    23941 attgtaggtt ttgtattaaa agacctttgc tattaattta agttagataa attgagcgat
    24001 aagcataaaa tgagaaaaca aataatagca gttaattttt attattaata ctaggtaaat
    24061 attaaatttt aaaataccac tagattttat agaataagaa aatgagacaa acataaatat
    24121 atgaatatat taaattatac ttgaacatac ttatatttta tggtaaaatt tcaataataa
    24181 ttattagtaa aggagtgttt tgcctgatgt cagagttata tataatggta accaacaaat
    24241 aaatagttgg agacgcatat gggttctgtt taatcagaat ttttaagtgt atttttgata
    24301 catttgattg ttgtgtctta ctctgccttt atagataact aacattagac aacaaataat
    24361 aatattacat attactttgt taaattgtgg tgtagtgggc tttaatctgc tacactttta
    24421 ttaattttac acagtattat tttgaatata attatgttga aatagaagat attaaatttt
    24481 ctagtccaca ggagttttct gtttttgtgt aggtaaatat gtataccaca taaaaaggca
    24541 gatataaata cctgtggact ttttttgtag aaaatacaag atacggagga aataaaaatg
    24601 aattataaaa atagaaaacg agcttattat caggaaaatt cctgtatgga taactttatt
    24661 tgcaaaaatt gtggttaact tgtagttcct gaaggagctg gtacacaaca tagaaatcat
    24721 tgtccacatt gtttacatag tcttcatgta gatatcattc ctggtgaccg tgctgcagat
    24781 tgtgatgcta atatggaacc tattggagtg tgggtgagaa aaaatggtga gtgggcaatt
    24841 attcatcgtt gtacacgctg tggacatctt agttctaatc gtattgcagc tgatgataat
    24901 tcagtgaaat taatgtcaat tgcattaaaa ccactgtcac agcccccttt cccactagaa
    24961 agaatcattg aaaagttgga aaaggaggaa acagaatgaa tggtatgaaa caaatcaatt
    25021 tacaagacta tggactaaat aggaatttta ttactcaaag tatggaatat catgatttat
    25081 accttgctcg aattatctcg caggaaaaga atttttatca agctgtatgt gtaaatggtg
    25141 tactgtttgc agaagtttca ggaaaactac gttttgaggc tactatacaa tctgattttc
    25201 ccacagttgg tgatttcgtt atgttggatc gttgcaataa taaaaatggt aatgctatta
    25261 ttcatcatgt gcttcataga aaaagcttgt ttgtgcgtaa ggctgctgga aaggtaaatg
    25321 aggaacaggt ggttgcagct aatattgaca ttgttttttt gtgcatgtca ctgaataagg
    25381 attttaacat tcatagattg gagaggtatc tgtctttaac atgggaaagt ggagctatcc
    25441 cagtagtagt acttactaaa tcagacttat gtgacaatgt gcatcgtaaa ttacaagtag
    25501 ttaattctat agccttgggt gtagatgtag ttgttacctc atctatagag ccagatggtt
    25561 ataaacaggt ttctaagtat attaaaccag gctgtactgt tgccttactt ggttcttcag
    25621 gagttggaaa atcttcatta attaattgtc tgctgggtgg agaattttta aatacaaagg
    25681 aaattcgcat agatgatgat aaaggaagac atgccacgac tagacgagag ctatttttaa
    25741 tttcaggtgg tggtattgtt attgacacac caggtatgag agagttggga atgtgggatg
    25801 tttcagaagg tttagataaa agttttgcag atatagaagc ttatttcgga aaatgtcatt
    25861 ttcgtaattg tactcacacc tcagaaccag gatgtgctat ttgggaagct attgctcatc
    25921 aagaactttc gcctaaaaga tgggaatcct atcgtaggct taaaactgaa gcagtatttg
    25981 cagacaataa aatcgaatac ttagcaaaaa aagaaaagaa atttaaaaat atctcaaaaa
    26041 taaataagaa gattagaaga taagtgtagt ttttttgttc attatatgag tctacatact
    26101 attaatatat taaatttgcc atcaatttgt tactttaatg tgattttgat ggcaaatttg
    26161 gtttgtggta ttatttaaag caatttatta atttttttaa taaagtttga ttttttatat
    26221 gatgatatat acatgtgata atgtatacat atttgttacg atgggctctt ttataaagag
    26281 gtcttatgta agataagaaa aggcatattt atactaatac ataatagagt atatgcttat
    26341 gtatgataga ggtgtttctt aaaagcaaaa ctgtaatatt actaatttat aaagtaaaaa
    26401 tacatgaatt ataaaatata aataagatat ttataaaaat tattgtttta atttataata
    26461 aaaatttttt gttgaaaata atgctcgaat ctataaaact attaactaat atttgtaaat
    26521 gtatactttt gatgatagaa tataattaag tt
//
LOCUS       DAEMIK010000043        25927 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEMIK010000043 DAEMIK010000000
VERSION     DAEMIK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.043"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            110..2758
                     /gene="polA"
                     /locus_tag="KPK87_001543"
     CDS             110..2758
                     /gene="polA"
                     /locus_tag="KPK87_001543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG2537699.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            2767..3369
                     /locus_tag="KPK87_001544"
     CDS             2767..3369
                     /locus_tag="KPK87_001544"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG2537700.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNWGTITELESKAHKFIEYMKENWRE"
     gene            3362..3916
                     /locus_tag="KPK87_001545"
     CDS             3362..3916
                     /locus_tag="KPK87_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG2537701.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            3916..5631
                     /locus_tag="KPK87_001546"
     CDS             3916..5631
                     /locus_tag="KPK87_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2537702.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5704..5916
                     /locus_tag="KPK87_001547"
     CDS             5704..5916
                     /locus_tag="KPK87_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG2537703.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6117..6716
                     /locus_tag="KPK87_001548"
     CDS             6117..6716
                     /locus_tag="KPK87_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537704.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            6944..7315
                     /locus_tag="KPK87_001549"
     CDS             6944..7315
                     /locus_tag="KPK87_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2537705.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            7447..8721
                     /locus_tag="KPK87_001550"
     CDS             7447..8721
                     /locus_tag="KPK87_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2537706.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            8832..9776
                     /locus_tag="KPK87_001551"
     CDS             8832..9776
                     /locus_tag="KPK87_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537707.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            9992..11329
                     /gene="rsxC"
                     /locus_tag="KPK87_001552"
     CDS             9992..11329
                     /gene="rsxC"
                     /locus_tag="KPK87_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG2537708.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11352..12329
                     /locus_tag="KPK87_001553"
     CDS             11352..12329
                     /locus_tag="KPK87_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG2537709.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12329..12898
                     /locus_tag="KPK87_001554"
     CDS             12329..12898
                     /locus_tag="KPK87_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG2537710.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDDLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            12911..13504
                     /locus_tag="KPK87_001555"
     CDS             12911..13504
                     /locus_tag="KPK87_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG2537711.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13519..14094
                     /gene="rsxA"
                     /locus_tag="KPK87_001556"
     CDS             13519..14094
                     /gene="rsxA"
                     /locus_tag="KPK87_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG2537712.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14110..15081
                     /locus_tag="KPK87_001557"
     CDS             14110..15081
                     /locus_tag="KPK87_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG2537713.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            15301..15885
                     /gene="maf"
                     /locus_tag="KPK87_001558"
     CDS             15301..15885
                     /gene="maf"
                     /locus_tag="KPK87_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG2537714.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            15930..16589
                     /gene="radC"
                     /locus_tag="KPK87_001559"
     CDS             15930..16589
                     /gene="radC"
                     /locus_tag="KPK87_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG2537715.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            16710..17720
                     /locus_tag="KPK87_001560"
     CDS             16710..17720
                     /locus_tag="KPK87_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG2537716.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            17730..18623
                     /gene="mreC"
                     /locus_tag="KPK87_001561"
     CDS             17730..18623
                     /gene="mreC"
                     /locus_tag="KPK87_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG2537717.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            18638..19126
                     /gene="mreD"
                     /locus_tag="KPK87_001562"
     CDS             18638..19126
                     /gene="mreD"
                     /locus_tag="KPK87_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG2537718.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAVIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            19138..22116
                     /locus_tag="KPK87_001563"
     CDS             19138..22116
                     /locus_tag="KPK87_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG2537719.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22214..22897
                     /locus_tag="KPK87_001564"
     CDS             22214..22897
                     /locus_tag="KPK87_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG2537720.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            22921..23718
                     /gene="minD"
                     /locus_tag="KPK87_001565"
     CDS             22921..23718
                     /gene="minD"
                     /locus_tag="KPK87_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG2537721.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            23736..24020
                     /gene="minE"
                     /locus_tag="KPK87_001566"
     CDS             23736..24020
                     /gene="minE"
                     /locus_tag="KPK87_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG2537722.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24105..25235
                     /gene="rodA"
                     /locus_tag="KPK87_001567"
     CDS             24105..25235
                     /gene="rodA"
                     /locus_tag="KPK87_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG2537723.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            25276..25689
                     /locus_tag="KPK87_001568"
     CDS             25276..25689
                     /locus_tag="KPK87_001568"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG2537724.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
BASE COUNT        10279 a         2823 c         5064 g         7761 t
ORIGIN      
        1 acatatttaa ttttaatata ctattattat tatgggaata ttaaataaca gtatattggt
       61 atataatata tatgttacaa aaatagataa tttttaggag tgatataatt tggaaaaaaa
      121 attaattata atagatggaa attcaataat aaatagagct ttttatgctc ttccagaaat
      181 gaataataaa gaagggctaa agaccaatgc aatatatgga tttacaacaa tgctatttaa
      241 aatgatagat atatataaac caacacatat aagtgtagca tttgacagaa aagcacctac
      301 atttagacat ttagaatata aagagtataa ggctggaaga aagggaatgc cagatgaact
      361 ggcagaacaa ttacagccat taaaagacct tttagataaa tttagaataa atagattgga
      421 aatagatggc tatgaggcag atgatattat aggtactgtt tctaaaaaag cagaagaaaa
      481 tggatataag gtatacatag taactggaga caaagatgct attcaacttg cttcagacaa
      541 tacaactact cttataacaa aaaaaggagt tggagaagtt gaagaataca actttaattc
      601 tgtaattgaa aaatacgaga tgactccaac acaatttatc gatttaaagg gtcttatggg
      661 agacaagtct gataatatac ctggagtacc tggaataggt gaaaaaacag gtataaagct
      721 tataaaagag ttttctagta tagagaatct aatagagaat acagataagc ttaaaggtag
      781 tgtaaaaaag aaaatagagg aaaacaaaga aatagctatt caaagtaaaa gacttgcaac
      841 aattataaga gatgtgccaa tagaagtgga tttagatagt atgatttttg gcgattatga
      901 taaagatgaa cttttagata aatttagata ctttggattt acaagtctct tatcaagatt
      961 agttgatttg gtcgattcag atgacacaga acacgtagaa gaaaaaatag aaatattaaa
     1021 attaaaagat atagaaaaat ttatagatga agtaaacaaa aaaggacaag ttattttaaa
     1081 aactgttaga ggacaaggta atatacttga aaaaaatata atatatattt ttataagtgt
     1141 agatggagaa aaaatatatt atgtagattc aaatgaagtg tcaaagttag atagtatatt
     1201 ttcaaataat gaaattaaaa agtatggata taatttaaaa gatgattata tatctttaaa
     1261 accatataat ataaacttag aaaatatgta ttttgatata acaatagctg agtacttaat
     1321 agattcaact tcatctacct catatgattg tagtgcaata gctatgaagt atttatcaaa
     1381 aaaggttaaa tcaaaagaag aattattagg aaagggtgtt aaatctaaaa actatgagga
     1441 tttagaattt gaggatttag cagagtatat gggtcaaata atatgcacag ttaaagaaac
     1501 agttcctatg atggaaagaa gtctaatgga tatgagtatg gatggtcttt tttatcatgt
     1561 agagatgcct ttagtagaag tcttaggtca tatggagtat gaaggtataa aagtagacaa
     1621 aagtatattg gatgaattga gtgttgaatt taaagaaata atagctacat tagaaaagga
     1681 aatttatgaa ttatcaggag agccattcaa tataaattca ccaaaacaat tgggtgttat
     1741 attatttgaa aaattagagc ttccaataat taaaaaaact aaaactggat attcaactaa
     1801 tgcagatgtt ttagaaaaat taagagataa gcatccaata atagataaga taactgagta
     1861 tagacaaata gtaaagttaa attctactta cgttgaagga cttttaaata taataaatcc
     1921 agaaagtaat agaatacatt catcatttaa tcaaactata acgactacag gaagaatttc
     1981 atctacagaa ccaaatcttc aaaatatacc tataaaaatg gaaatgggta gaaaaataag
     2041 aaaagtgttt attgcagatg ataactgtaa gttggttgat gccgattact cacaagtaga
     2101 gctaagagtt cttgcacata tgagtcagga tgaaaatatg atagaagcat ttaaacatca
     2161 tgaagatatt cataccaaaa cagcttcaca agtttttaat gttcctatgg aagatgttac
     2221 tagtgaattg agaggtgcag ctaaagctgt aaactttggt attatatatg gtaaaagtga
     2281 ctttggtcta gctgatgatt taaatatacc tgttccaaag gcgaaagaat atatcgaaag
     2341 ttattttgct aaataccata aaataaaaga gtttatggac acaacagtag aaaaggctag
     2401 tgaggatgga tatgctgtaa caattttaaa cagaagaaga tatataccag aaataaagtc
     2461 aagcaatttt atggaaagaa atagaggaaa gagatttgca atgaatgcac caatacaagg
     2521 aagtgcagca gatataataa aaattgctat gataaatgtt cataaaaaat tggaagagaa
     2581 caatttgaaa tcaaaactta tactacaagt tcacgacgag ttgatagttg aagcaattga
     2641 tgatgaaata gatatagtta agaaaatagt aaaagaagaa atggaaaatg ctgtaaatat
     2701 ggatgttcac ttagatgtag atttaaatgt aggtagttct tggtatgaga caaagtaggt
     2761 gattttatgt tgatattagg acttacagga ggaataggct gtggaaaaag cagcctatca
     2821 aatatattta gaaatcttaa cattcctata gtggatgctg atattatatc caggaagata
     2881 tttgaggata agttgttatt ggaaaaagta tttgtccatt ttgggcaaag tataaaaaat
     2941 gatgatggaa ctctaaatag aaaagctctt gggaaaatag tttttagtga tgaagaaaaa
     3001 cttaaggaat taaataactt gactcatcca cgaataagag aaaaaataat aagtgaaata
     3061 gaaaaattga gaaaaaaagg cgaaaatata gtagtgttag atgcggcaat attggtagaa
     3121 agtggttttt tagatatggt tgacaagcta ttagtagtta cttgtaaaca agaagtacaa
     3181 attagtagaa tacaaaagag agataattgc agtgagcagg aagcacttag taggataaat
     3241 tcacagatga gtcaggaaga aaaatccaaa tatggtgatt atataataga taattgggga
     3301 acaataactg aattagaaag taaagcacat aaatttattg agtacatgaa ggagaattgg
     3361 cgtgaatagt aagaaagtat tgattctatc tatttttata atcttatttg gggcactatt
     3421 aatggaaagc aaagtaatac ataaattttt atatcctaaa aaatattcag agtatgtaga
     3481 aaagtattcg aaagaattta atttagatga aaatatagtt tacagtgtta ttaaagccga
     3541 aagtaagttt aatagttctg ctgtttcaaa aaaggaagca aaaggattaa tgcaaatatt
     3601 agacataact agagattggg gagcagagga actaaattta aaaaatgtgg atattttcga
     3661 cccagagact aatataagac ttggctgttg gtatttaagt aagttataca aagaatttgg
     3721 taaattagat ttagtgatag ctgcatataa tggtggttca ggtaatgtga aaaaatggtt
     3781 agaaaataat gaatatagta aagatggcga aaatctacat gatatacctt ttaagcaaac
     3841 ttcaaaatat gtagaaaaag taaaaaataa ttacgaacat tataataaga tatatggcaa
     3901 gaaaggaaaa aactaatgaa gagaataaaa gtcttaacag tcgttttagc tataactctt
     3961 atggtagctg gttgtagtaa tactaaaaca aaacaaagta gtagtgattc aagtttacaa
     4021 agtcaaagtt cgatagatgc taagtatata aacttaacaa tggtaactcc aaaaactata
     4081 aatcctataa ctaatactga taagtctgtt ggttatataa tgaatttagt ttatgatagt
     4141 ttatttacta tagatgagaa ctacaatgta attcctcaat tagttaagga gtataatata
     4201 gctcaggatg gaatgagtat agatataaag ctaaaagatg caaagtggca tgatggaaaa
     4261 aatgttactt caaatgatgt aaactatact attggtttaa ttcaaaaaag tgcagatagt
     4321 ccatataatg aatttactaa aaatatagct tctgtgaata ttaaatcaga taaagatttt
     4381 acaataaaat ttaaagctag atatgctttt tcaattgata gtttaatttt tccaattgta
     4441 tctcaaaatc atttagattc aaaagatgta aatgacaaga ataataatat gataggaaat
     4501 gggaaatata aaatagaatc atatacagaa agagaaggaa tggttttatc tgtaaacaaa
     4561 gattactatg aagaagttcc aaaaaccatg aagaacataa aagtaggtat ggtgcctaat
     4621 gaagatgcaa ggacatctat ggttatggct cttgatagtg atataaccaa tgttacatta
     4681 aatgatttaa gtaagtttca agaaaaagag tttaatataa caaaatatca aggaagagat
     4741 tatgagtgtg tattatttaa ttataataat ccttttttca aagatgttaa ttttagaaaa
     4801 gcaattgcac actctataga taaagataga attattagtg aaggttatat ggatgatgct
     4861 actccagtta attttccact taattcaaaa tcaaaatact ataatagtga aatgaaggat
     4921 ttagagttta ataaagataa ggcgatagag tgtcttgcta aggtagaata tgcaaatgta
     4981 aactcagtaa atcaaaatga caataaagta aaaaatcaga aaaaatcagc taaaaagaac
     5041 tcaaagaagc ttacgccaga agaagaagca aaagctaaaa aagcagaaga agatagaatc
     5101 aaaaaagaag ctgaagagaa aaaaaataga gaaaaagagg aagtaaagaa aagtctttct
     5161 gaaatgaact taaagataat agtaaacaga gataatagtg agagaataaa aactgcaaac
     5221 ataataaatg aaaacttaaa agctatagga ataaatagta cagtaaatca gctgtcagat
     5281 aaggatatgg aaaatgctct taattctaaa aattatgatt tagcattagt tggatggaaa
     5341 ctatcaatta taccagatgc aagtagtatt atagcgagta gtggatatac tgatgataag
     5401 ttaaatggat atatgtctgc acttacaagt tctacgtctg aaattgaaac aaaaaaagca
     5461 tacaaagatg tacagacata tataaaagat aatgcaacat ttataagttt agctgtgaga
     5521 aacaactata ttgtaagtaa tagtagatta aaaggtaaaa ttactccaaa tgattttgat
     5581 gtatatgagg gaatatctaa tttagatata aaatcgaatg aatcaaatta aatgattaat
     5641 ttctatttaa aaagtataaa ctatatgata gaataccctt atagggtata aggaggtaat
     5701 taaatgaaga agaaactatt agtagaaggt atgagttgtg gtcattgtgt aaaccatctt
     5761 aaaactgcat taacagaaga tatagatggt attgaagttt tagatgtaga tttagaaaat
     5821 aagtgtgcat ctgtagatat gaaagatgat gtatctattg aaaaattaaa agaagtgata
     5881 gcagaattag gatttgaact aaagggaatt gaatagaaat tgataaacta aaaaaagacc
     5941 tctattgggg gagtaataga ggtctaaaaa tgggggagat tgagtatgtt tgattttaca
     6001 ttactattat ctcccctttt aaattaatat atacataaaa agtaaaaaaa ttttgtaatg
     6061 actcacattt cttgagcttt ttgaatatac tataaatagc taagggaggg agtatcatgg
     6121 aatctagagg atataaagag gatttaaaaa aaatgatact tgatatggcc cacaaagaac
     6181 ttgagagtta tgtatcacaa aaaggagcaa aaaaatactt tgaaggtaac ttggataaga
     6241 ttattaataa taatataaaa aaattcaatc aaagtattgg tcttaataga gtatatttaa
     6301 aaatgttaaa aaagtgtgca tatcaagaaa gtgtaacttc aatatcaagg actataaaga
     6361 aagaagcatt atttaaatca aaaaaagagc tatttgcttt ttcagagtac ttaaaacttg
     6421 atattaacaa gcgactttca tataatcaaa ttttaagaaa aatatcaaga tatatatact
     6481 acaatagaag ttcatatgct caaaaatacg ttgttttcaa aagaggagat gaagaatatc
     6541 ttttagaacc agagaaaata aaagatgaat taatatcaag ctataggtca aagactagag
     6601 aagatatgag gtcaatagca aaacttttgg atatagaagt agaagatgat tataatgctg
     6661 aagatattag aaaaaaagta ataaattata ttatcaaaga aaaacttaaa agttagagtt
     6721 ttaaagaata agttctgcca atttgcatga ttaagtataa cttgggtata aattttttat
     6781 gcatttgaaa gagcttattc tttttttgac atattctttt aaataattta ccatgatttg
     6841 gtaattggtc atcatcattc aaatttttaa cttaatatga tttaaataaa tttataataa
     6901 tgatataata taaaaaaaat aaaaattgga gagtgatttc aaaatgaagt ttaatttttg
     6961 tcataacaat ttcaatgtta ctaatttaga aaaaagttta gatttttata aaaaagcttt
     7021 agggcttaaa gaggtaaagc gcaaggaagc agaagatgga agttttatat tagtttattt
     7081 aggagatggc attactagtc atacattaga attaacttgg cttagagatt gggatagacc
     7141 atataattta ggagacaatg aatttcatct ggctttagaa gttgaggagt ttgatgaggc
     7201 aaaaaaactt cataaagatt tagattgcat atgttttgaa aatgaaagta tgggaattta
     7261 ttttatagcg gacccagata attattggat tgaaatactt cctaaaaatc actaatttta
     7321 aggatatgtt tataatttct ttacaattaa taagaaaata agttatcatt aaattgtagg
     7381 gactaaaaat ggataataat gttatgaatt tatgactata aaaatttgtt ggggggagtg
     7441 atagtagtga aaaaagctat agcagcttta ggaattgggg cagtagcagt atctgttagc
     7501 tccataaatg caagtgctct tgaaaaggga acagtaacag caagtgcctt gaatataaga
     7561 agtggaccaa gttctgattg cgataaggta gcaaaattat acaagggaaa aactgtagag
     7621 attttagaaa agtctaatgg atggtataaa gtaagggtat cgagttctgt tgttggatgg
     7681 ggaagtgcaa aatacatatc aacaagtggt tcgtctgaag gtacatcaag tcaaaataat
     7741 tcaacttcaa gtgggacaac tattagtgga aatggtaaag taaatgtgag ttctagactg
     7801 aatgtaagaa gtggtgcagg tactaactat tcattagttg gtaaagcaaa taatggagat
     7861 gtagtaaagt tactagaaca aagcaatgga tggtataaaa taaaactatc aaatggagtt
     7921 actggatggg ctagtagcca atatatttct aaaacatcag aggatgttgg aacgaataat
     7981 tctagtaatt caaatagtac taataattct gataaaaaac catctagcga agaatcaatt
     8041 gaaggcaaga atggtaaggt aacatctgct gtgagtttaa atgtaagaag tggtcctggg
     8101 acaagctatt ctataatagg aaaattaaat ggtggagatg ttgttgaatt aaaagctaaa
     8161 agtaatggtt ggtataaggt aaagttatcg agtggaacaa ttggatgggt gagtgccagt
     8221 tatatttctg agactaatga ggatacaaaa gaaaaaccaa attcatcatc taaccaaaat
     8281 tctcaatcta atagtaactc aaagccatca tttactggaa attcagataa atctacagca
     8341 aaaggttcaa caatcgtaga ttttgcatat actcttattg gaataccata tcaatgggga
     8401 gcttcaggac ctgataaatt tgactgttct ggatttacac agtatgtttt taaacattct
     8461 gtaggcgttt ctattcctag agtttctaga gaacaggcta attttggaag tgcaatttct
     8521 atgggaaatt atgcaccagg agatttagtt tattttgaca ctgatggtga tggaacaact
     8581 aatcatgtag gtatatacgt aggaaatagt aaatttatcc attgtagtgg tactcagact
     8641 aatcctaata aggttaaagt ggataattta actagttcat attggtctaa agtattactt
     8701 ggtgcaagaa gatttgttta gtaagtacaa tttcttatta aaaaattaga agaaataatt
     8761 atttatattc ataaattgtg actcttaaaa atatacttta ttaataatat aattttggga
     8821 gggacaaact aatgaataga agaaaaacga tatacagagg atttaataat aatagacata
     8881 agattgattt acgtaaatat gttataacga ttgcttgctt gtgtctaatt ggctattatt
     8941 cttatacaaa aataaaagat agcaagatat tggagtatgt atcggctaaa ataccatttc
     9001 taaataattc atcagatata acttataaag atatatctga tgaattaaat tcaataaaaa
     9061 aagggaaaaa atctaaatca caaaccaatt cagatgataa acaagaaact aatccagaaa
     9121 aagcggtaaa taacactaaa gagccagaag aagtcaaatt agctactata gaaggctggg
     9181 atatgtatac tattcaagtt gctgcaatag ataataatga tgatttgaaa aaaatacaga
     9241 catcattagt taataatgac ataccttttt cagttatgga gaaggatgga gtaaaaaaaa
     9301 tacaaaccta ttcctctttt gatgaaaatg atgttagaaa gcagataagc agtgtaagaa
     9361 aggtgtttcc agatgcattt ttatcacact tagatgcacc tatgttgtct ttagagtata
     9421 caagtaacta cgcttatata gagagtatat ctaaagaatt aaataaatta ataacaaact
     9481 ttaaagaaga atcaagtttt tggtcgaatg ctgaaaataa tgtagatatg gaaaaatata
     9541 atactatttt gacaaataga aaggcaatat cacaaaatat atcaaaagaa gctgaaaaga
     9601 ttgactattc tgaaatgcgt ttatttaaag ataatttaat agagtatgta aagaatgtga
     9661 atgaaaaaat tgatacagct tcaaaagcag caaatgaaga aaaatatagt gtaagtaaaa
     9721 gtctgctttt atcatccatg caggagtact ctatgtttat aaattctata aaataaaata
     9781 cagaaaattt ggactttaga aagctatatg aaaaggattg tttaattaag caatcctttt
     9841 cctcggtttg acttaatata gaggtattga tttataaaat aaattataaa aatacggata
     9901 tttttttaac aattgttaag attttttaat atatatagta tacttataga aggcagaaaa
     9961 aatgtttttt tatagataag ggggtaaaac aatgaaactc ttaactttta agggagggat
    10021 acatcctcca tatagaaaag agtactctaa cacaaaggca cttgaaaaag ctcaagctcc
    10081 aaagatagtt tacattcctc ttcagcaaca tataggggca ccagctaaac ctattgttga
    10141 agttggtgat gaagtaaagt ttgggcaaaa aataggtgaa caacaaggtt ttgtttcatg
    10201 taacgttcat tcttcagtat caggtaaagt tattgctatt gaacaacatg aagtaccagg
    10261 aggttcagct caatgtgtag ttatcgaaaa tgactttaag gaagaattac atgagagtgt
    10321 tcaaccaaaa ggtcagttag aggatttgag caaagaagat atagtaggta tcataaaaga
    10381 ggcaggaata gtaggtatgg gtggagctac attccctaac catgtaaaag tatctcctcc
    10441 accagatagc aaggcagaag tagttatatt aaatggtgca gaatgtgaac catacttaac
    10501 tgcagaccac agattaatgg ttgaaaatcc agaagatgtt gtgtttggtt taagagcatt
    10561 aatgaaggta ttagatgtca aaaaaggatt tataggaatt gaaacgaata aaccagatgc
    10621 tatagaagca attcaaaatg tagctaaaga ctatagcgaa atcgaagttg ttggccttca
    10681 agtgaaatat ccacaaggag cagagaaaca acttatttat gcgtgtacag gcaaagaagt
    10741 tccatctggt ggattaccaa tagcagcagg tgcagttgtt gacaacgttg ccacagcagc
    10801 acagatagct aagtctatta aaacaggtat gcctttggtt gaaagaatca ctacaataac
    10861 aggtagttgc atcaaagagc caaagaacct aataacaaaa gttggaacat tagtttcaga
    10921 aataatagaa caatgtggtg gatttaaaga agataagaag attggtaagg ttataatggg
    10981 tggacctatg atgggaatag ctcaatatac tactgaaata gctactaaca aaggttcttc
    11041 aggaatttta tgtttggatg aggaagaatc acgtactcca gatattcaaa actgtttaag
    11101 atgtggaaga tgtacagatg tatgtccatc attcttacaa ccacttttca taagtgcata
    11161 ttctttaaaa gatgactatg acacagctga atatcataga gcaatggatt gtatagaatg
    11221 cgggtcttgc tcatttattt gtccagcaag aagaccatta cttcaatcta taagatcagc
    11281 aaagagagaa ataggagcaa agagaagaaa gcaagctgct cagaaataaa gaaagttaag
    11341 ggggaaaaac gatggaaaat aagttgatag tatcatcttc tcctcatgtg agaagtaatg
    11401 aagatacttc atatataatg aaacaagtta ttatagcact ccttccagca gcagtagcag
    11461 gagtatactt ctttagactt aatgctttga gtgctatgtt tttttgtata cttggtacag
    11521 taggtactga atttttatat caaaaattta cgaagcaaaa aagtactata ggagattttt
    11581 cagctgttgt aacaggatta ttattagcat ttaacgtacc agcatcactt ccttggtgga
    11641 tgtgtctagt aggaggaata tttgcaatat tagtagttaa aatggtattt ggtggaattg
    11701 gatgtaactt tgtcaatcca gcacttgctg caagagcatt tttattagca tcattcccag
    11761 tagcaatgac tgcttggact caaccaggtg ttaactggat aggtaaaaat ttagatgcag
    11821 ttactacagc aactccatta agctttttga aaaatggagc agcaggactg gcagatcttt
    11881 ctagcaatgg aattagtctt gctgacatga tgatagggaa tattggtgga tgtataggtg
    11941 agacatcagc aatattatta ttattaggtg gagtatacct aatgtataaa ggcataataa
    12001 attatgttat accagtattt tacatagcaa ctgtatttat attaacattc cttttaggtg
    12061 gatttaatat aactttcgca atatatcaac tgtttgctgg aggacttatg ttaggtggat
    12121 tcttcatgct tacggattat acaacttcgc ctatgactaa aaaaggtcaa ataatatacg
    12181 ctgtattagc tggtcttata acaactgtta taagaatgta tggtggatat ccagaaggtg
    12241 tatcttactc aatactatta gtgaactgtc ttgctccact tatagataag tttgttagaa
    12301 atagagtatt tggggaggtg gctaaataat gaacagtatg gtgagattag gtggtacttt
    12361 acttgctata agtgcaatag ctgcattagc tcttggagct actaatcagg ttacagctcc
    12421 agtaatagaa caaagaaata tacaagcaaa taatgaactt agaaaagcag tccttccaga
    12481 agcaaaagaa tttaaagaaa tgaaggaaga tacttacaaa ggccttggag atgatcttat
    12541 agctgaagtg tatgaaggtt tagatggttc tgaagtggtt ggatatactt taaaagctaa
    12601 accaagtgga tatggtggag aaattgaagt tatggtaggt atatcatctg aaggtcaagt
    12661 tacaggtgtt gacattggta atatgtcaga aacagcaggt cttggagcaa aagctaagga
    12721 tgatgcattt aagggtcaat acaaaggaaa aactgctgag cctcttgaag tagcaaaagg
    12781 aagtacaaca gcagataatc aaatattagc aatatcaggt gcaactatta catctacagc
    12841 agttactaca ggagtaaatg ctgctataga tgttttcaat agtgctctta ataaataagg
    12901 agggttaatt atgaatttag ctaaagtatt taaaaatggg ctaatagatg agaacccaac
    12961 atttgttcaa gttataggta tgtgtccaac actagccgtt acaacttcag caataaatgg
    13021 aattggtatg ggtctttcaa cagcagcagt tttaatatgt gcaaatttag ttatatcttt
    13081 aataagaaaa attacaccag ataagataag aatacctata tttatagtag ttatagctac
    13141 atttgttaca atagtaggta tgcttttaaa agcatatgtt cctgctcttg ataaggctct
    13201 tggtatatat ataccattaa tagttgttaa ctgtttaata ttagctcgtg cagaaagttt
    13261 tgctttcaaa acaggagcta tgccttcaat agttgatggt gtaggtcaag gtcttggatt
    13321 tactgttgca cttacaataa taggtgcagt aagagaatta cttggaaatg gtagtttatt
    13381 tggaatgaca ttatttggag catctttcca accagtatta atatttatat taccaccagg
    13441 tgcattctta acattaggat tcttatttgc aggatttaat aaattaagaa gtaaaaaagc
    13501 atagtggagg tgtaataaat gaatttaata cttttatttt taagtatcgt tcttgttaat
    13561 aacgttataa catctcaatt cttgggtata tgtcctttct tgggagtttc taagaaggtt
    13621 gatacagcag tagggatggg agttgcagtt acgttcgttt taactctagc ttcaattata
    13681 acttatttta tacagatttt attagtaaaa actggaactg gattcttaca aacaatagct
    13741 tttatattgg ttatagcttc tatagttcag ttcgttgaaa tggtaattca aaagatgagt
    13801 ccatctttat atcaagcatt aggagtttat ttacctctta taactacaaa ctgtgcagtt
    13861 cttggtatag ctctagttaa tgttcaaaaa agttataatt tagtagaaac aataataaac
    13921 ggatttggag ccggagctgg atttacttta gcaatagtaa tattcgcagg tataagagaa
    13981 agactagaat tagctgatat accagaagca tttaaaggtt tccctataac gcttatatca
    14041 gcaggtttaa tgtcaatagc tttcttagga tttacaggac ttataaagct ataaaaaaga
    14101 ggggtgaaaa tggtgatact tacagctgta ttagttttag gaattatggg cttaatcttc
    14161 gggatagtac tagattttgc atctaaaaag tttgctgtag aagtagacga gagagtggaa
    14221 gctatactag gagtacttcc aggagccaac tgtggaggtt gtggatttcc aggttgtgga
    14281 ggtcttgcaa atgcgatagt tgaaggcaat gcaccagtta atggttgccc tgtaggtgga
    14341 gcagatgttg gtgcaaaagt tggagaaata atgggaataa gtgctgaagc aggtgagaaa
    14401 caagtagcaa aagttatttg taaaggaact tgttcaagtg caaaagataa atatgagtat
    14461 gaaggaatat cagattgtag agcagcaaat gttttaaact caggtgctaa gatgtgtaaa
    14521 tttggatgtt taggtcttgg aacatgtaaa gatgcttgta aatttgatgc tatttctata
    14581 gttgatggaa tagcagtaat agatgaagaa aagtgtgtaa attgtggaaa atgtaaagaa
    14641 gtttgtccta aggggataat aatcacaaaa ccagaaagtc aagaagtagt tgtagagtgt
    14701 aatagcaaag agtttggtaa agctgttaag gaaaaatgta ctgctggatg tattggctgt
    14761 ggaatgtgtg taaaagcttg taaatttgat gcaataatat ttgaagacaa aatagctaag
    14821 atagacccta ataagtgtgt agggtgtatg caatgtgtag caaaatgccc tacaaaagta
    14881 atatcaggtg acataactaa gaaaaagaaa gttactattg accaagaact ttgtgttgga
    14941 tgtacagtgt gcaaaaaaca atgtaagttt gatgcaatag agggagaatt aaaagaaaaa
    15001 cataaggtag atgcagataa atgtgtagga tgccatttat gtatggaaaa atgtccaaag
    15061 aaagctatta aaattttgta atatggacaa aataaggata tggaataatt tccatatctc
    15121 taatgagtac ataaaacaga ggtatggaat aatttccata cctctatttt atgtatattt
    15181 attctaatgg taataaatat aacaatagtg taacaaaaaa ggttcgacat ttctgttgaa
    15241 ttttttacaa tatagtgata gactaaatag gtacaaatta cgtacaaatg aggtgaataa
    15301 atgaatataa tcttggcatc agcctctcca agaaggaaag aaattctaga gaatacgaat
    15361 gtaagatttg atataattaa aaatgaaatt gatgagatta tattggaagg tgaaacacca
    15421 aaacatttag ttatgagatt agcttttgaa aaaagtatgt ctgtagcatc tgaacataat
    15481 gaagatgttg ttattggagc tgatacagtt gttgttttag acaatgctat tttaggaaaa
    15541 ccaaaagatg agtcttgtgc aagagatatg ttaaaaagac tatctggaag agagcatcaa
    15601 gtcataacag gtattagctt aataaacttg tgcgaagata aaaaggtaat tgactatgta
    15661 atcagcaatg tgaaatttaa aacgttatca gagcaggata ttgaggatta tctgaaaact
    15721 aatgagtcat ttgacaaagc aggagcctat ggtatacagg gttatggagc tttgttagtt
    15781 gaagaaatta gaggagatta ttttaatata gttggtcttc caatttcaag gttaggcgac
    15841 ctattaaaaa aatattttag tataaatctt ttttatggag tgtgatttat ctgaaaaagt
    15901 catttaattc aaccattaag gtaaaagaaa tggcaccaga ggaaagaccc agagaaaaga
    15961 tgcttgccaa aggcgtaaaa agcttatcaa atgctgagtt gttggctata cttttaagaa
    16021 ctggcaacaa gaataaaaat gctattgaat tagccaacta tataataaat agagatattc
    16081 agggtattag acatttagaa gatatgacaa tagaggagct gtgtaatata gatggaattg
    16141 ggttatcaaa atcaacccaa attaaagcag ctttagaact aggctctaga gtagctagtt
    16201 ttaaacctat aaagtataaa ataaggaatc cttgggatat acagaggtat tatatggata
    16261 gcctaaggta tttaaaaaaa gaggttttta aagcggttct tttaaacaca aaaaatgaga
    16321 taatatctga tgtagatgta tctataggga ctttaagctc gtcattagtc catccaagag
    16381 aggtttttaa agaagctata agaagaagtg caagtaagat aatagtgatg cataatcacc
    16441 cttcaggaag tgtagaacca tcaagagaag ataaaaatat cacatcaaga cttattaaat
    16501 gtggagaaat aattggaata gaaataatag accatataat tattggagat ggattatact
    16561 ttagctttaa agaaaatatg ataatttgac tttatatgtg ataaaataat aggagaatgt
    16621 tttaaacatg attcttaata gtaggaagga gtaaaatcat ggctaaggaa aaaaagaaag
    16681 aaaaaaaagg tttttttagt tttaataaga tgacaaagga tatgggaata gatttaggga
    16741 ctgcaaatac attagtttac ataaaaggac aaggtattgt tgttagagaa ccatcagttg
    16801 tcgcaataag agatgatagt aaagaggtat tagcagtagg tgaagaagcc aaaaaaatga
    16861 taggtagaac accaggaaat atagtagcta taagacctat gaaagatgga gtaatagctg
    16921 attttgacat aactcaatca atgattagct attttataca aaaagcggct gataaaaaag
    16981 gtgttgtaag tccaagaata gctatatgtg ttccttttgg agttacagaa gtagaaaaga
    17041 gagctataga agaggctgca agacaagcag gagctaaaga tgcattcctt atagaagagc
    17101 caatggcagc ggctataggg gctggattaa aagttgaaga accagaaggt aatatggtag
    17161 ttgatattgg tggaggtact tcagaaatag ctgtaatatc tttaggagga atcgttactg
    17221 ctaaatctat aagaataggt ggagacgaat ttgacgagtc tatagtggct tatgtaaaaa
    17281 aagaatataa tcttatgata ggtgaaagaa ctgctgaaaa tgtaaaaatt aacataggtt
    17341 ctacatttaa agatgatgaa gaaataaata tgcaaataag aggtagagat ttaatctcag
    17401 gattacctaa aacaatagaa atatgctcta cagaagttag agaagcatta aaagaacctg
    17461 taagttcaat agtagatgca ataaaatcta cactagaaag aactccacca gaattagcct
    17521 cagatataat ggaaaacgga ataatgctaa caggaggagg cgctttgctt agaggtcttg
    17581 ataagcttat tacacaggaa actggtatgt ctgtacaaat agcagaaaca cctcttgatt
    17641 gtgttgcact tggaacaggt aaatctgttg aagaccaaga aatctttgaa aaagtattaa
    17701 tgatgaatac taagaattag aagttggtga tggccttgag atttgacaag aacaaaaatg
    17761 agaaaaaaag gattaatgtt aaagtgatag caacgggcgt agttgctatc actttaattg
    17821 gaattgtggg tatatcaatt ggtaaatttt ctagtggtag tccagtaaat ttaggagtag
    17881 cttcagatgc tattacaagt gtaggtaagg gaataaatga tggattttct tttatcaaaa
    17941 atggttttaa agatgtagca aattttaaag ataattctaa aaaggtaaaa aaattagaag
    18001 aagaaaatga aaaacttaaa aaaaatatga tagctttaaa tgccaaacta gataaaacag
    18061 aatcgctaga agagttaaaa aagacactga actttgtaca agaagagtat aaggcgacaa
    18121 gtatttcagc aagtgttgtt ggtaaaaatg atggaaactg gtatgagagt tttgtcatag
    18181 gtgcaggaaa aaattcaggt gtaaaaaaag aaagtatagt tatgaatggt agtggattag
    18241 ttggcatagt ttatgaagtt tccaacaatt atagtaaggc aatttcacta ttggattcta
    18301 aggcatctgt aagctttaaa ctagcaaagg atgccaatgc taaaggtaca attactcaaa
    18361 atactacttt agataataaa gatagttata atagtaaagg atatttgcaa ggatatatgt
    18421 ttgactcatc atataatgta attcaaggag atataataac aacatcaggt cttggattct
    18481 tcccagatgg tataccaatt ggcgaagttg aaaaagtggt tgatgataaa gataagtcat
    18541 taaaatatgt agttgtaaaa ccttatgttg attttaagaa tataaatgac gttgtagtca
    18601 tagaaccaag aaatatagga taaaggggat aaaaacaatg aaaaaagttt tactttgtct
    18661 gttggggatt gtgcttatga ctatagaaaa tagtataaca aattatatag atatttttgg
    18721 cataagcttt aatttagttc ttatatatgt aaccataatc tctctttatt tagatgaatt
    18781 agaaggtggg attatagcag taataatagg gcttattaaa gacataaccg ttggtggaat
    18841 ttttggtgta aatgcattaa ttttgtttat tattgcatat gcaattggat acatgagaga
    18901 tcagctatat aaaggtagtt atattattac tttcgtttta atttttatag gcagtttaat
    18961 tgattcaatt gtcaatatag gtacatctag tataatttat caaagctaca acatatcaac
    19021 actttttgta aaaggtctac tagtagcacc aatagtgaat agtttaattg gtttagtaat
    19081 atatcgctta tctaaaagag cagttttaaa acttaaagaa gattagttgg tgatttcatg
    19141 gaagaaaata aacaagttga tagactaaaa ataattaaga tagtcatggg cgtgatattt
    19201 ttagctattt tagtaaaaat aatctatatg actacattta aatatgaata ttacaatgag
    19261 ttagctgaga ataaaacata taaaaagttg gcaatagaag ctccaagagg agaaataaaa
    19321 gatagatatg gaagactgtt ggcaggaaat aaaaacttat ttactgttca agtttcaggt
    19381 aacgatatta ataagaaaga tgcaaataag cattcaagag caaatgaaat atcattaaaa
    19441 cttataaatt tgctggagag aaatggtgaa gaatatgtag atgagtttcc tatatatgta
    19501 gaaaatggaa agtactatta tacatatgac agagatatta gggaatataa atcagaaaat
    19561 ggtattccaa atgattataa tgctaaggaa agtttttatt acttggtgga caaacttata
    19621 tcagctggaa tattatctca agaagataaa agattggatg cgacaagatt acaggcaaaa
    19681 ctaaatgaaa atggatatta tccacctatt ttagttagta aatggatgtt cacagcagaa
    19741 agagataaga gagattggtt ggctagttat aagataaaag aaactaaact gagtgccaaa
    19801 gaagcgtttg aaaaagtaag aaatagtgat gcattagaaa ttgataaaag tttgagtgat
    19861 gaagatgcaa gaaaaataat ggtcgtaagg gatttgataa aatcaaaagg atattctcaa
    19921 tataatccag taactattgc taaggatgtg ggagaaacta cgattgctca aattgaagaa
    19981 agtgctatgg accttgttgg tgtttcaata gcagtagaac cagtaagata ttatcctaat
    20041 gggtcattag cttctcatat gttaggatat gtaggaaaaa tgccatccac tcaaatagaa
    20101 tcataccttc aaaaaggtta tgaaactgga gatatggtag gtcttgcagg agtagaaaag
    20161 agtaatgaaa gtagattaag aggaactgat ggttataaaa tggttaaagt tgatgcattg
    20221 ggtagaatta gtaaagaaat tgaatctaaa aaacctaaat caggagatac tgtgtatctt
    20281 acactagata aagatttaca agaagtttca gataatgcat taaaacagat aatcgaagtt
    20341 gcttctaaag gaggtacttt taaaagtaaa tttggggaca aaccaattag tgcatatgca
    20401 ggaaaagcac agtcagcagc tcttatagct attgatgtta aaaatggaga agtattagct
    20461 tcatcaagtt atcctaatta tgacccaaat aaatttgcaa aaggtatttc tacagaggat
    20521 tataaagcac ttcaaccaaa aaatccaaat gacttactag caggaagtcc acttttaaac
    20581 ttggttacac aaggggaatt tcagccaggt tcttcattta agatggtaac ttcaatggca
    20641 gcgttagaaa atggtctgga tccaaacttt actataaatg accctggggt tataatgtta
    20701 ggtaaaaaat cttttggtga ctatgtatgg aaccatggta gaggaaacca tggtatgaca
    20761 aatttatata aagcaataca agagtcttgt aatatatata tggcaacaat tggtacaggt
    20821 aaaacatggc cagatggaaa aagcattggt atagatatga atgcaaataa aatacttgag
    20881 tatgcaaaat tatttgggct tgaccaaaat acagggcttc aagatgaagt agaagaaaga
    20941 gcaggtaaag ttcctagcac agaagataaa ttgaaatcaa cacaagctct tctaaagtca
    21001 aatttagaaa gagaaatggc aaatgatttt gttgatataa ctagagaaaa aaatcctaaa
    21061 gaatatgaga aaagaatcaa tgagattgta agctgggcag cagaaaagaa aactcctggt
    21121 agagttgaaa caatgaatag gctaaaaaaa ttggatgtta aagaagatag aattgaagaa
    21181 gtagcagatt tagcagtatt cagttatttt aactttgcaa aatggagtac tgcagataca
    21241 ttcaacttag ctataggaca aggtgagaat gcctatacac cagctcaaat atcaagatat
    21301 gtagctgcta tagcaaatgg aggtaattta gtagaattat ctgtagttga tagagctgtt
    21361 tcaagtgatt attcatcagt taaaattaat gaccagaaga aagtcgaaaa aatacctttt
    21421 aaaaatccag ataacttaaa agaattaaca aaaggtatga aattggttgc aagacaaggt
    21481 acagcgaaga gtgcttttgc tgattttcca atagatgtag cagcaaaaac tggtacagct
    21541 gagaaaagtg ggaaaatacc tacagataac gagtatgaat atttaaaaag tcatatgtca
    21601 tcttataatg tcaacctaaa tgatgcgatt aaactagctg ataagatgaa agcagaaaaa
    21661 gaaaaagagc tttcattagc taaggaaaag gaaataaaaa agaaattaga aaataaagat
    21721 ttgaaagacg aagaaagaaa gaaattagag gaagaattag aagatggagt aaaagttaga
    21781 ttagaggaca ctgataaggt gaattcttca tatctaagaa aagctataaa agaattaaat
    21841 ccaaaaataa cagatgacca aatagataga tttaaacaag attatggttc ttttacgtgg
    21901 actgttgctt ttgctccagc agatgaccca gaaatagctg ttgtatgtgt aattcctcaa
    21961 ggtgattcaa gtgtattctc acttttacca actagagaag ttattggaac ttatatggga
    22021 ttagagccaa ctaatagtaa aaatgacaac aagacgaatg atgttaacaa ttcttctgac
    22081 gaaaatataa atttcgagtc acaaattaat agatagctca atctatgtat aaactttagc
    22141 tttatataga aaaaataaaa gggtttaaag cgtatttgaa gaatataaat gatatatcac
    22201 tctggaggtt gttatgtctt taagagaaat ttgtagtcaa gaactagtag agttcaaggg
    22261 taataaaaga gggataatag taaatataaa aagagaagct ccatttgagg aaatacagga
    22321 aaaaataata aacaaattgg aagcatatgt tggctttttt aatggggcta aaattagtaa
    22381 aattaatagt gattgtttaa ctgacatgga gattctagaa cttaaagaag gaattacttc
    22441 tagatttgat gtggaatttg ttgaagatca aaaaattgaa gaaaatagta attttccaac
    22501 aaaatatgtc aataccctaa gatctggtga aaatattgaa tttgaaggtg atgtagttat
    22561 attgaatgat atgaaacctg gttcaaaagt cctttcaaaa tcaaatacag ttgttatggg
    22621 agatataaat gctggtgcta aggttgtagc aggaggtaat gtttttgtaa tgggtaaaat
    22681 agaaggtttt gtacatgcag gtgcagaagg caatgaattt gcatatgttg ttgctggaaa
    22741 tcttaatcct aaaatattac aaatagcaga taatattgca gaagctccag atgatgaaga
    22801 aaattatgaa agtgaatcag aaattagccc agaaatagcc tttgtaagta atggaagaat
    22861 tgtaatcgaa agctatttgt caaagttaga caaatagaaa gaaaaatagg gggtagtatt
    22921 atgagcgaag ttatagttat aacatctggt aaaggtgggg ttggaaaaac tactaccgca
    22981 gcaaacttag gaactgcgct aagcctagaa aataaaaaaa cagtagttgt ggatgcagat
    23041 attggactta gaaacttaga tgtagtaatg ggtcttgaaa atcgaatagt ttatgacata
    23101 gtagatgtag ttgaaggaac ttgtaggctt aagcaagctc taataaaaga caaaagattt
    23161 gataatctat atttattacc agcagcacaa actagagata aaaatgctgt ctcagttgag
    23221 cagatgattg acctgtgtga gaaactaaaa gaatctttcg agtatataat aatagattgt
    23281 ccagcaggta ttgagcaagg ctttaaaaat gcagtagctg gggcagatag agctattgta
    23341 gttactaatc cagaaatatc agcagtaaga gatgctgata gaataatagg tctattagaa
    23401 gcaaatgaaa taaaagagat aagattagtt ataaatagaa ttagaaatga tatggttaag
    23461 cgtggagaca tgatggataa acaagatata atagaaatat tagcaataga tttgttaggt
    23521 cttgttcctg atgatgaaag cataattata tcaacaaata aaggagagcc agctatactt
    23581 gattctaagt cacttgctgg tcaagcatac aaaaatatcg caaaaaggat actaaatgaa
    23641 gaagttcctc tacttgatct tgaagttgaa gatggattct ttggtagact taaaaaaatg
    23701 tttagcatgg ctaagtagca aaggaggata catctgtgtt agatttattt agagtttttt
    23761 ctaacgaggc taaaactagt aaatctgttg ctaaagagag gttaaagcta gttttagttc
    23821 atgatagagt agattgttca ccacaacttt tagagatgat aaaaacagac attttaaaag
    23881 taatagctaa ttatgcagaa atagaagatg atggtcttga aataaaaatg tctaaatgca
    23941 gaggtgagca tgatgacaaa cctgtatctg cattagttgc taatatacca ctaaagaata
    24001 taaaagatag atgtatgtaa ttagtgctta aaagggggat atccttattg gtatatgccc
    24061 cttttattta attatatgtt taaagataga aagacggtga ttctttgaaa aaaatcaata
    24121 ttaattataa gtcgacaatt aagttgataa aacagttaga ctggaaactt atagttacag
    24181 tattagctat ctttattttt gggcttgtta tacttagtag tgcaacacat gctaattcaa
    24241 caggttcata taatcaattg attaaacaag gattagcttt tgtacttggt ataggaatga
    24301 ttatagtcat attatttttt gactataacc ttctaggcag gtattataaa gcgctttata
    24361 taataagtct tattttgtta gccatagttt tattacctgg aattgggaca gtaaaaggtg
    24421 gagctaggtc gtggattaac ttaggaccac ttgacttgca gacatctgaa atagtcaaat
    24481 taacatttgt actaagttat gcaaagatac ttgaatccaa aaaggataaa ttaaatacgt
    24541 taaaggaagt tatgcctgtt gtagtttatt ctcttccatt tatcggatta ttgattgcac
    24601 aaccagactt gggaacagga attgtatttt gttgtatgat atttgcaatg ctgttcacag
    24661 caggtcttag ttcaaagttg ataaaaagag gaataatcat actattagtg tcaatgccac
    24721 tgatgtattt aatgatggca gaccatcaaa aggtaagaat agaagctttt ctaaatccag
    24781 aagatgtaac tctaaaagga aactatcaag ttatgcaatc tctgattgca attggttctg
    24841 gaggcgtaac tggtaaagga ctttacaatg gaagtcaaaa tcaagaagat tttttacctg
    24901 tacaagatag tgattttata tttgctgttg taggtgaaga acttggtgta ataggaatgg
    24961 cagtgttgat aatcttattt atgatattcc tattaaggct tttagcaatt gcaagagatg
    25021 caaaagattt ttatggaacc ctcatagtgg ttggtgttat gggaatgttt ggttaccaga
    25081 taatccaaaa tataggtatg acggttgcgt taataccagt aactggagta acattacctt
    25141 ttgtaagtta tggaggaagt tcgttattga catctttagc aaacttagga ttagtactaa
    25201 atgtgtgtat gagaagaaag aaaataaatt tctaaattta aatacatcta aacataaatt
    25261 gaggagatta gatatatgaa tatagcatta gtagcacatg accaaatgaa aaacactatg
    25321 gtcggatttt gtataggtta tgaatcaatt ttaaaaaagt atggactata tgcaactgga
    25381 actacaggaa aaagaataat ggatgaaact gaattaaata taaatagatt agcatctggt
    25441 cctttaggtg gagaccaaca aataggttct ttaatagtaa cacaagaaat agatttagtt
    25501 atttttttaa gagacccatt gacatctcag gctcatgaaa cagatataca agcgttaata
    25561 agactttgtg atgtttatca tgttccaata gctacaaatc tagcttcagc agaaatattt
    25621 ataaaagctc ttgatagagg agaactatca tggagagaag ttagaaaaag caaaagtcaa
    25681 cgtatttaaa aaatattaat attacagtct aaagaaacag atatataagt gtttaatgcc
    25741 ctagtctttc tgttgcaata aacctagtac ctgtagaaat cttatgaaag atttaaatga
    25801 tgaagagcta aatttgagat taaataatta ggaaatagat tatagataga gtagtagaat
    25861 ttaaactatt actctatttt aataatatga aatagaatca agtctattat tatatatatt
    25921 taaactt
//
LOCUS       DAEMIK010000044        25621 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEMIK010000044 DAEMIK010000000
VERSION     DAEMIK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.044"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..913)
                     /locus_tag="KPK87_001569"
     CDS             complement(<1..913)
                     /locus_tag="KPK87_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2537725.1"
                     /translation="MFILKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNI
                     DKFKSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYT
                     FGNLDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLE
                     FVNLEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCF
                     NKFKESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEMMKDISKKMDTIDKL
                     KDIEEGYFYLLEDVYFDMKDVLEDLMLY"
     gene            complement(952..1812)
                     /locus_tag="KPK87_001570"
     CDS             complement(952..1812)
                     /locus_tag="KPK87_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG2537726.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2081..2290
                     /locus_tag="KPK87_001571"
     CDS             2081..2290
                     /locus_tag="KPK87_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG2537727.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2290..2556
                     /locus_tag="KPK87_001572"
     CDS             2290..2556
                     /locus_tag="KPK87_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG2537728.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2585..3157
                     /locus_tag="KPK87_001573"
     CDS             2585..3157
                     /locus_tag="KPK87_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2537729.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3392..4582)
                     /locus_tag="KPK87_001574"
     CDS             complement(3392..4582)
                     /locus_tag="KPK87_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2537730.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4842..5273)
                     /gene="dut"
                     /locus_tag="KPK87_001575"
     CDS             complement(4842..5273)
                     /gene="dut"
                     /locus_tag="KPK87_001575"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG2537731.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5386..7614
                     /locus_tag="KPK87_001576"
     CDS             5386..7614
                     /locus_tag="KPK87_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG2537732.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNDDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7725..8210)
                     /locus_tag="KPK87_001577"
     CDS             complement(7725..8210)
                     /locus_tag="KPK87_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2537733.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8462..9685
                     /locus_tag="KPK87_001578"
     CDS             8462..9685
                     /locus_tag="KPK87_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG2537734.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9689..12823
                     /locus_tag="KPK87_001579"
     CDS             9689..12823
                     /locus_tag="KPK87_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG2537735.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSSGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12857..14155
                     /locus_tag="KPK87_001580"
     CDS             12857..14155
                     /locus_tag="KPK87_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG2537736.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14444..14689)
                     /locus_tag="KPK87_001581"
     CDS             complement(14444..14689)
                     /locus_tag="KPK87_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2537737.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(14903..17530)
                     /locus_tag="KPK87_001582"
     CDS             complement(14903..17530)
                     /locus_tag="KPK87_001582"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG2537738.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLAALEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17591..18433)
                     /locus_tag="KPK87_001583"
     CDS             complement(17591..18433)
                     /locus_tag="KPK87_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG2537739.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18440..19078)
                     /locus_tag="KPK87_001584"
     CDS             complement(18440..19078)
                     /locus_tag="KPK87_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537740.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19514..20806)
                     /locus_tag="KPK87_001585"
     CDS             complement(19514..20806)
                     /locus_tag="KPK87_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG2537741.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(20843..21199)
                     /locus_tag="KPK87_001586"
     CDS             complement(20843..21199)
                     /locus_tag="KPK87_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG2537742.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21234..21593)
                     /locus_tag="KPK87_001587"
     CDS             complement(21234..21593)
                     /locus_tag="KPK87_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537743.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21662..23635)
                     /locus_tag="KPK87_001588"
     CDS             complement(21662..23635)
                     /locus_tag="KPK87_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2537744.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(23857..24870)
                     /locus_tag="KPK87_001589"
     CDS             complement(23857..24870)
                     /locus_tag="KPK87_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG2537745.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(24883..25428)
                     /locus_tag="KPK87_001590"
     CDS             complement(24883..25428)
                     /locus_tag="KPK87_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG2537746.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         8777 a         4258 c         3264 g         9322 t
ORIGIN      
        1 tataaagcat taaatcttct agtacatctt tcatgtcgaa gtacacatct tctaataaat
       61 aaaaatagcc ttcctctata tctttcaatt tatctatagt atccattttt ttacttatat
      121 ctttcatcat ttctaaattt tgtatattta catctatgat tgttaataag tacattctaa
      181 cagtttttat tatttcaaca gaaaaatcta caatattata atttccttct tttttatata
      241 atgactcctt aaatttgtta aaacaattat ctaaattaga atttatatcg ttaaatggtg
      301 gtcttattat gtcacgtatt attaaaagat tttgtatgtc tacattattt gtgattgcct
      361 catccaaaat aaataaatta tatgcatcat ttaatgggtc atgggcatct ccactaaact
      421 ctaagttaac aaattcaagt aaatttttca atgatatata gttcatacat ctcataccac
      481 aatttatata tttttctttt atatctacaa ataaatctct aatgttattt acaaaccttg
      541 gatggttatt ttttttagca cttctcatat ctgtgttatt gaaacatgtc aaatccaaat
      601 taccaaaagt atatattcct ttaatatcag aaaatatgcc aagccatttt ttaaactttc
      661 tcatgacctc ttcataactt ggtgcactct tcaattcttc agaagatata tgtgttaatt
      721 cttgtatatg aggatacaac tcctcattac ttactggtct tattaaagat ttaaatttat
      781 caatattttt tgttttttca tcatacataa ctgctccgat tgcaattata tcagaattaa
      841 atactgctct ttttgattta gaatttggca tattcatttc aaaatctata tatactcttt
      901 tcaatataaa caccctttcc aattgtttat agtgattctt tttaactatt tttattcaaa
      961 attttctaag aaagaatgtt tctccccatc tttttgccaa ccaagtactg aatcaagatt
     1021 tacattttct gccgccttaa atatagattt ttctacattt ttaaaatttg attctaaact
     1081 ctttattaag tcttttatct catatctttt attacctgtt tttttagcag caaccataca
     1141 tgcacaattt aatggccata taccactatt ttgaataaat cttataatat gttcttctct
     1201 tatataataa agtggtctta taatttttat accttcaaaa ttagtagaat taagtttagg
     1261 aagcattgtt ttaaaatttc cagcacacaa taagttaagc attgttgtct ctattacatc
     1321 gtcataatga tgacctaatg caagtttgtt acaccctaat tcttctgcct ttgaatataa
     1381 tgctcctctc ctcattcttg cacacatata gcatggataa tcttttgcta tttcatctgc
     1441 tatctcaaat atccttgaat caaataaatg tattggtata tttagatact cacaattatc
     1501 tataagtaat tgtcttatat ttgcatgata tccagggtcc atagcaataa attcaacatc
     1561 aaaattaact tgtccatgtt tttttaactc ttgaaacatt ttagccataa gaatactgtc
     1621 ttttccacca gaaatcgcta cagctatctt atcaccttct tcaaccaatt tataatctct
     1681 tattgccttc ataaacttag accataaatt ttttctatat ttttttatta tacttttttc
     1741 tatatctctt actggaagtc tttcctcaaa aggaactata atttcacagc cttttcctga
     1801 tagttcactc atctaatcac ctcgtatatt tatgttaata atattttaca aaaaagtcta
     1861 ccttcatcca caaaatacta taatttttac tttaatttta ctaaataatt tatagtttac
     1921 aataacacat aaacttataa taacataaat aacctctttc ttttattatc ttttttattt
     1981 ttctcattta atattttatt attagtaacg atttttaaat gattgaatat tatacactag
     2041 atagagttac ctatctattt acaacaagga ggttttcatc ttggaaaata agactagaga
     2101 tatcaaaaaa aattgtggtc catctttggc tagaccatac attaataatc aaattttcac
     2161 agaaatgtac tgcctttctg atgcattaaa atttggaact atatttccag aactaaactt
     2221 actagagtct gaaatgtaca acaaagaatt atatgctaag ccaaaaaaat atagaggagg
     2281 tagaagataa tgaaagactc atctagagct gaacttatga gaaaagttca ggaaacatct
     2341 tttgcatgtg ttgatatgaa cttatattta gacaatcacc ctgatgataa aaatgctatc
     2401 aacacttata attcattatg taatcaattt gcgcaagcta gatatgctta cgaaaataaa
     2461 tacggtcctt taactaactt tgggtatgct ccaagtagat gcccttggca atgggctgac
     2521 caaccttggc cttgggacag agaatttaat tattagacat caagtattgg aggattgaaa
     2581 attcatgtgg atatatcaga aaactataca acacccagtt aatataaaaa cttgtgaccc
     2641 tagaatggct aaatttctta taactcaatt tggtgggcca aatggagaac ttgctgcatc
     2701 tttaagatat ttaagccaaa gatatacaat gcctactggt aatatgcgtg cacttttaac
     2761 agatattggt acagaagaac tagctcacgt tgagcttata tgtactatgg tttatcagtt
     2821 aacttctgat gcaagcccag aagagttaaa agctgcaggt cttggttcaa actatgctca
     2881 aaatggatat ggaatttatc caacagattc aaatggtgtt ccatttgatg taagacctat
     2941 agcagttatg tcaaatcccg taaccgattt acatgaggat atggcagctg aacaaaaagc
     3001 acttgcaact tattatcaac ttataaacct aacagatgac gttgatgtta tagatgtatt
     3061 aaaattcttg ggtcaaagag aaataattca ctatcaaaga tttggtgaag ctttaatgga
     3121 tgcttacgag ttagaagaat ctcaaaaaat gttctaaaat ttttatttgt ataactatta
     3181 ttatttgata atttataatt tactgttgtg ttaaaattta taaaaaatat aaatataatt
     3241 tattttctat aaacagagaa aataaataaa aagcaggtat caatcaaata gtaacatgta
     3301 ttatactata cattactatc aatattgtac ctgctttcat attataagca ataaagaaaa
     3361 atctatttat taggttttat aataaaaaca attataaaac tcttcttgca tttacatatc
     3421 tacttgaata gtagctacta tttatattag atattgttac tttttttgat gaacctgaag
     3481 atgcatgtat catatctcca tttcctatgt atattccaac atgacttaca gaaccattat
     3541 ttgaaccttg tgtatcaaag aatactaaat ctccacttct aaggtctcct ctatttacat
     3601 atgttccata cttactttgg tctctagata ctcttggtat actaactcct acagattttt
     3661 tcataacgta ctgtgtgaat ccagaacagt caaaactatt tggaccttct gctccccata
     3721 catatggttt tccaagtagt gtctttgcaa aacttattac actatcagct ccttgacttg
     3781 gagaattagt cgaataagat ggaattgaag tatcaacaga acctttttca gcaaggtatt
     3841 gacttgacac atatccagtt tgattgccat aacttacttt tgtccatcct ccactttcag
     3901 aaatacttcc aacctcagta ccataactta atgtagctat tttagaatga cttgtacctg
     3961 gtcctgttct aacgttcaat gactttgctg ttacaacctt tgtacccttt acaaggtcat
     4021 ttgaactgtt ttcagcattt tcatttgtag agtcatttac actagttcct aaatatttac
     4081 ttgaaacata acctactcta ccattagaac ttatttttga ccatccattt gactcagata
     4141 tgtatccgac ttcagtccca tatcctagtg ttgatatttt agaactactt gtacttggtc
     4201 ctgttctaaa atttaatcct tttgctgtta caacttttgt cgacttaact gaagtatcac
     4261 tactttcatt gctacttcca aggctattta ttgctacata atttccatgc acataacctg
     4321 tgtttccatt atatttaact tttacccaag aaccttcttt tcctacgtac tcaactttat
     4381 cacctttgta aagtttcccc attgattcat ggtttgtacc tggtcctttt ctaaaattta
     4441 ccgcattccc tgttattgtt ctgctttcta tactcacact ctctacttca gactcattag
     4501 catatgctaa attccccata gtaggcatta ttatcgcagt tgccattata ctggctgcta
     4561 ttctcttttg attaaaattc ataactttcc actcatctcc tcttttctac tatctttatt
     4621 tcacttcaaa atataaaaga aataattgac actattaaaa tatcaattat ttcttttata
     4681 ttaatattat tacaaatttg taactatttg tctatagtaa tatttgctta ccataaatta
     4741 ccatgtataa attttaatct ttaatagcct gtaatttagt aatatagcca tcttttacct
     4801 tttttaaaaa tggctatatt ttttaacttt tttgtaactt tttaaaatcc agttgagcca
     4861 aacccacctt ttcctctttc ggagtcactt aattctttta cctcttctat gttaatatca
     4921 tagataggtt ttactatcat ttgagcaatt ttcatatgct tttcaacctt aaaatcattt
     4981 ttaccatgat ttattaatat aattttaagc tctcctctgt atccttcgtc aatagttcct
     5041 ggtgtattta atacagtaac agaatgcttt aaggcaagcc cacttctagg tcttacctgc
     5101 gcttctgtca tagtaggaag ctctatacat atcccagttt tgataagttt agtctctcca
     5161 ggaggtatta caacctcttc tatagaatac aaatccatac cagcatcacc tttgtgagca
     5221 aaattaggta ttatagcatc atcatttaat ttttttactt ttaaattgta catcttatat
     5281 ctcctttcaa ctttctataa atattataac ttaaatttcg acctcttgaa gttttttatt
     5341 tttttgtggt aatataatta ttagtgtttt aaaggaggat tttttatgaa acttgtactt
     5401 gctgagaaac catctgtggc aaaaactata gcatcatttt taggtgcaaa gaccagacaa
     5461 gatggttact ttgaaggaaa tgattatata attacttatg cagttggaca cttagtatca
     5521 ttatatgata tgaaggatta tgataaagat aaatactctg gctcttggaa aatgaacaat
     5581 ttcccatttg ttcctgagga taagtttaaa tttaagattg atagttctaa aactaaacaa
     5641 ttcaacactg taaaaaagct tttgaataga aaagatgtag aatatgttat aaatgccact
     5701 gataatgacc gagaaggaga acttatagca tttttaatat ttttactggc aaaaaataaa
     5761 aaaccagtaa aaagaatctt ggtaaatgaa tggacaccag aagatattac aagaggtata
     5821 aaaaacctaa aagatgaaga tgaaatgaga aatcttcaag ctgctggata tactaggctt
     5881 attactgact ggttgattgg aataaatttc acttctgttg ctacattgaa atacggaaat
     5941 gggaaactat taaacatagg tagggtaatt cttccaacag ttaaattagt ctatgataga
     6001 gatatggaaa tactaaactt tgttcctaaa acttattatg aaatagaagg gcattttaaa
     6061 gctgaagctg gtgaatataa aggtaaatat gttaagggta aagaaagtaa atttgataca
     6121 cttgaagatg caaataaaat tatagcttca attacatctg aaactggtaa aatactagat
     6181 aaaaaagtta ctatgtcaaa ggaatatgca ccaaaacttt ttagtttgac atcacttcaa
     6241 ggatatataa cttctaagta ttccaatttt acatctgata aggttttaaa tgtctgtcaa
     6301 tctctctatg agggtaatgg taagggtgga tacataactt accctagaac tgattccatt
     6361 ttcttagaag aaagtttggc ttcaaaagct tctcaaactt tagataaatt aaaggttggt
     6421 cttgaatatg aaaataaaat taaattttct aaaactaaaa gagtttttga ttcttctaaa
     6481 gtagactccc atagtgctat aattccaact tatataattc caaaaagttt atctaaggat
     6541 gaacaattag tttatgatgc cataaaagat agatttgtag caaactttat gcctcctgct
     6601 gaatatgaga atacagaaat taagactgaa gtagataatt gtactttctt aacaaaaggt
     6661 aaggtgctta aatcaaaagg ataccttgag gtatataata aggaagaaaa aaacgattta
     6721 cttccacttg taaataaaga tgatgttgta gatgtgttag aaataaaacc attaactaag
     6781 caaactactc cacctaaacc atatactgag gacacccttt taaaggctat gaaaaattgt
     6841 ggtaaaaatg tgcctgaaga agatacaaca gtgctatctg gatattcgat tggaacatca
     6901 gccacacgtg ctgatgtgtt aaagaagata agtcaagtag gctatgtaaa gaaaaaaggg
     6961 aaatcttatt ctataacaga ccttgggaaa aatttagttg aaatttttcc tgttaaagac
     7021 ttatttgatg tagattatac tggaaaactt gaaaaaagtt taagcgatat tcaaaaagga
     7081 aagtatacaa gaaaagaata tcttactaat atcattagtt ttatatatca aaatgtcaat
     7141 attataaaac aagatgcttc aaaaaatata aacacagaag catatacttt cgattcaaaa
     7201 actaaaaaat ttactaaaac aaaagaaaaa aaacagaaaa aagatgtaac taatactgaa
     7261 aaagaagttg ctatatcaaa ggattctaat aacaaaaaag aagttaaaaa tgatgatgaa
     7321 aatcaatctc ttggcaaatg ccccatttgt caaggtgatg tactagaatt tgacaaaggc
     7381 tttgcttgta aaaatcataa agaatgtaaa tttgttattt ggaaaaatga taagtattta
     7441 agtctttata aaaagaaagt taacaaaaca atggtcaaaa atattttaaa aaaaggtgaa
     7501 actaaagtaa aatctttaac tgctaaaaac ggtaataaat ttgatgctac attaaaatat
     7561 gataaaaatc cagataccgg ctatttcaat tggaaaatag agtttgacaa ctaaatattc
     7621 ataagaaaag aaatagcagt agtataataa tctatattat attactgcta tttcttttct
     7681 tatgaagttt taactttttt aatagtgact tttacaatca cactctaacc taatttcttt
     7741 actatttctg ataactttaa tgtatgttca tctaataaag ttagttcctc ttcagaaaga
     7801 ttagataatt tttttactag agattctttg agaatagctt ttactgcatc aaataattta
     7861 aatgctttat ccgttaattc aaccctaaga atccttctat cttttgggtc aggaaattta
     7921 ttgactaaac catgttccac taatctgtca ataatcggag tcatatttga ttttaaaatt
     7981 cctaaattag aagctatttg agaaattgat gatgaatttg atgtcataag ataaagaatt
     8041 accttagcat gtgatggagc cattggacat tcttcaaaat aagctttcat tatatcagag
     8101 ttattttgaa acttttttaa caaatcgctt tctttaaata tattattttc aagcacaaat
     8161 aaaaaatcta aaaaagagtc cgctatttta agtatattgt tgttatccat atttgtaaca
     8221 tcccttcaat agataatata ataaaacatt gttgtttaat atagtataaa ttataaacta
     8281 ttaataaatt taaaacaaga aaaagttatt gactcataat aattataaat ttataatcaa
     8341 attagagaat tataaattgc caataaaggg aagtttttta attagtctaa aactaaactt
     8401 aaattataag acactttaaa ttctacaaca agctagtgtg caatatataa taggaggaga
     8461 tatgaaaaga tatttaatag ttttcttaat gtgtagttca ctgtttttag ttggatgtgg
     8521 aaaaacagaa gaatctgaac ctgagaaacc aatagcagtt tctgttcaaa aagcaatagg
     8581 tggagaaatt gaaaacacaa attctttctc aggaactaca aaggttaaag atgaaacagc
     8641 tgttactgca cagactgttg gcacagttca agaagtgtat gtaaaacttg ggcaaaatgt
     8701 aagaaaagga gatgaattat tgagtataag cagtccagag ctagaaaata gtgtaaagca
     8761 aagtaaagcc tcactagatt tagcaaaagc aagttattca agtgccactg gtggttcatt
     8821 agaggcacaa gtaaatcagg caaaaacagc tcttgacaat gcaaagattc aatatgagga
     8881 gtctcaaaga aattatgaca ataataagat tctttatgaa caggaagtca ttagtttaga
     8941 ccaatttaaa aaaatagaat tttctttaga gcaaacaaaa caacagttag attctgctca
     9001 aagagcatac gatacagcaa cttcaaaaag tatacctcaa gctaaagcac ttgctaaaaa
     9061 acagcttgac caagctcaag tatcatataa tttagcaatg agtaatttag acaaattaac
     9121 attaacatct ccagtagatg gtacaataac agctaaaaac tttgattcaa aggaaatgat
     9181 aacacaatca caaccagctt ttattatttc taatcctaat atactagaag ttgatttaaa
     9241 tgtagcagaa tcagatatag gtaaatttaa gaaagatgga aatgtagatg ttataattga
     9301 agaccaaagg attttaggaa aaatagatta tgtaccttca gtagttgacc ctcagacatc
     9361 tttatatcct gttaaagtac ttgtaaataa tacaaacaac aaatttaagg caggtatgtc
     9421 tgctcaagta aacttaagtg ttgaaaaaga aaatggtgct gttacagtac ctaaaaaagc
     9481 aatatttgaa gaaaatggta aaaaatatgt atacatagca acaaaagaca atatagcaaa
     9541 gaaacacttg gttcaaactg gaattgtaac agaggataaa atagaaatta aaagtggtgt
     9601 aagtgataaa gatacagtga ttattggagg gataagtctt atttctgacg gaactaagat
     9661 atttcctgta gaaaaggaga agtagactat gaatttaaca cagacttccg ttaaacgtcc
     9721 tctcacaatc ataatggtct ttttagtatt aatagttttt ggtggtattg gatataaaaa
     9781 aatgtcaata aatctaatgc ctgatataga aataccagtt gtaatggtca tgactacttg
     9841 gacaggtgct ggtcctcaag acgttgatga acaagttagt caaaaagttg atgaaagttt
     9901 atctgccgtt tcaaatgtaa aatctactat atcaagttca caagaaagtg tttctatggt
     9961 agttgctcag tttgaatttg gaactaattt ggatgaaata atgaatgatg ttaggtctaa
    10021 agttgatgca ttacagacca gcctcccaga tgatgcagct aaaccaacag ttttaaagct
    10081 ggatatgaat gcacaggcta taggtcaact tgtcatatct ggaggaaatg aaaatagttc
    10141 acaagcctta agaaaatacg ctgaagatgt aattcagcct aaaatagaat ccatagatgg
    10201 tgttacttca gcagacctta aaggtggaga aaaagcacag gttaatgtaa tagctgaccc
    10261 agctgttctt tcaaattatg gagttagttt atccactata aaaggtgtct tatcctctag
    10321 taataaaaca ttcccttatg gttcaattac tcaaggtgag gataaaatag ttttaagagc
    10381 gattgataaa ttagaatccc tagatgatat aaaacagatt caaattcctg taaaaggagg
    10441 aaatacagtt agattagata atctttgtag tgtagattat ggatatgcag acaaagatag
    10501 tatttataga tacaatggaa aagacagttt ggtaatagat atttctaaac aacaagatgc
    10561 caacactgta aaagttatgc agagtgcata caaatacgta gatgaattga ataaggaaaa
    10621 tcctcaattt aaaattaaaa ttgctaatga tacaagttca tacataagtg aatcaataaa
    10681 cagtgtaatg agcaacttgc ttataagtgc tgttatcgca tttattgtaa tatttgcttt
    10741 cctaaaaagt gtaagagctt cactagttgt tgcagttgca ataccaattt ctataatagg
    10801 tgctatagca attctctact ttactggtga aagtttaaat cttattactg caagttcatt
    10861 ggtaatttca gttggtatgg ttgttgataa tgctacagtt gtaattgaaa atatatttaa
    10921 atataggaaa agtggtagat tgagtcttga tgattgtgct attgagggaa caagaactgt
    10981 aacaaatgca atacttgcat caacattgac cacaatagca atattcttac ctatcatgtt
    11041 tacagaaggt atagctaaaa taacttttgg ggcattaggt aaaacactaa tagcttcact
    11101 agcattctcc tttataggtg caattacact cgttcctagt gtatttgcaa aattaagcag
    11161 cggaaaaaat agtcaaaaga tgcaagaaaa agatagtcct atatttgata aggtaagtga
    11221 agcatataaa aaattattaa gtgtatgttt gagacatcaa cgcttagttg taatatcaag
    11281 tttacttatt tttgtagctt ccttatttgg aatgggattt ataggtatgg actttatgcc
    11341 tgcagcagac aagggtgaat tatctatttc tatagaactt ccaaaaggtc ttagcctaga
    11401 atccaatgat tactatgtat ctatgactga gaaaaaagtc tcagatattc cagaaattaa
    11461 aactttaata acaactttga catcagattc aaataatagt aaagcaagta tagcaattga
    11521 attagaatct caaaaaaata gaaaaagaac tactgatgaa atagaaaaag agataattga
    11581 tagagtggct actgtaccag attgtaaaat aaatgtaagt caaaacagta gtgttatggg
    11641 tggagctgga ggagcagact tcacattaga aatgaaaggt cctgatttag atgtattaaa
    11701 aatactggga aaagaagctg aaaaagaatt tagctctata aaaggattta gaaatgttga
    11761 aacttctctt acagatacaa cacaagaagc tcaatttact atagataaac ataaggctca
    11821 agaatatggt gtaaataccg ctgaaattgc aggcatgtta cgtacagcaa taaatggtga
    11881 ctccgtaacc actgctacta ttgatgatta caaagtggat gtaaacctaa aatttaaagc
    11941 agatactatt gatagcttag aagatataaa gcaactaaaa gtaaactctg caactggaaa
    12001 agaagttcct cttggtgcct tttcagatat aaaaatggca gatggtctac aatctatatc
    12061 taggactgat ggagattttt ctgtaaatat aactgctaca cttgacaatt tagatgctgg
    12121 tactgctaca aaactagcac tagaaaaaac gaataaaatg gtaattccta aagattactc
    12181 tataggtgtt ggtggtgaaa cagaaatgat gaatgaatct atggcaagtc ttggattttc
    12241 aatggtcata gctatagcat tagtttatat ggtaatggta gctcagtttg aatcatttag
    12301 taaaccattt ataatcatgt tctgtgttcc atttgcattc gttggagttg tgctttcgct
    12361 tctagttagt ggtcaaagtt taagtactgt cggtatgcta ggtataatct tacttatcgg
    12421 tattgttgtt aataatggta tagttcttat tgactacata gagcaactta gaaaaacaaa
    12481 tcgtaacaat gatttaattg gtttggtttc aattggttgt gcagcaagat taagaccagt
    12541 cttaatgact acagcaacaa caattttagg tatgttgcca acagcactat ctcttggtga
    12601 aggtggagaa actatgcaac cactagccat agttataatg ggtggtctaa ctgtatctac
    12661 acttataact ttggtgctta tacctactat atacttaata tttgataaat gggaaaataa
    12721 gttttcagac aaaatgccta aaatatttgg taagataaca gataaaatag gaagtataaa
    12781 acgtaaaata ataaagaaac caaaattgaa agctaaagtt taaatagaat ggagggtgat
    12841 tgtattgaat aaaaaaatga gcaaaatagt tgcaattggc tgtggtgttg gtataataac
    12901 tgccaattta atcccagttt acgcagataa taatacttct tctataaaca aagttgataa
    12961 ttctatttta agtgactcaa ataaaagaac atctgaaaag gaaattttga ctgctagaga
    13021 agctgttagt gctgcactta aaaatagtga aaaattaaaa atgaaatcag aagaaataaa
    13081 aatgttaaaa gaaaagttag aagtacaaga tgagtttgat agttttactg gaagtgacaa
    13141 ctcgtttcca tacgaccaaa ttggacttct taaaaatcaa agtgaacagg ctaaaggctt
    13201 tatggaggac caaatagcaa atgatattac taataaattt aacgatttag tttcaagaga
    13261 aaatgagcta gataaaataa aaaataatct tgaaataaaa actaaagaaa ttaaagatat
    13321 gaaattaaaa aaagatttag ggttagtaac atctttagaa actgaatcag ctgaacttga
    13381 acttcaaaca ctacaaaata ctcaaaaagc taaacttcaa gaactaaaaa acaatcaaga
    13441 ctattttaag ttattaacta atatagattt aaataattat caattggata aagaatatag
    13501 atttgaatct tttagagtag gtggctcagt tgattcatat atggaaggta aggttaatga
    13561 atatttaaaa tacgaccaat tgattttaga acgtacagaa gaaagcttta atgataaaga
    13621 tgagaacaaa gcagatttac cagatagacc taaccttaca agaccagttg ctcctacaaa
    13681 accagagcaa ggtaacctaa ctgatgatga atacaaggtt cttatggata agtatgaaaa
    13741 agattatgat agatataaaa aagaagttga agcatacaat cttgaaagac agacttatgc
    13801 tgctggcctt actacttatg caaattatct tcaacaaaaa tttaatactg aaaatggtct
    13861 agtaacatta gaagacagta aaaaagctct taaaaaaggg ttaattgata gctatgctca
    13921 attacttgct ttagaagata caattcagat tactaaaaaa caacttgatt tatcagaaaa
    13981 acaattaaaa aatactaaat taagatatga tttaggatta attactctaa ctgattataa
    14041 aaaacaagtg gtaagtaatg aagatgctaa aaatagctat gacactctta ttgtgaatta
    14101 taactcacta aaaaatggta tagaaaaacc ttggatactt aatactggaa aataattctg
    14161 gtaaatagaa agattacttt aacatacgtt tgattaaaaa atagttatgc atactcgtgg
    14221 ctttagtcat ggaagattca gaaggcaaat aatagaaata gattagtaaa aatatattta
    14281 cttataaaac tgtatacttt tatagaggct atctcaaaat agagataagc ctctttttta
    14341 ttgtttaaat tataaaaata tatttgattc catacaaaaa gagtgcctta tgaactaaaa
    14401 agttcatttt gagacaccct caaaagctta actctaaatt taattatttt ttatcaaatg
    14461 tctcatctat tttacatctc accatgttaa caaattcaga aatattatat cctctaagtt
    14521 tgcatccaca tttaggacaa taatccagtt tcctagatac caaagcccta caactaggtt
    14581 ctggacatct tatttttaga ctctcatctg ctactttaaa tccacatcgt gggcatatac
    14641 tacctggacg tgatgatact tcctttccac atttaggaca ctttgtcaac tttatcccct
    14701 ccagtaaact caatttaatt gatagttgaa atttctctct acaataatat atgcaatata
    14761 attaaatacg tttcacttac aactatataa gttaaatttt tataataaaa atcccctata
    14821 ttcaatttac aaatacttaa tttgatacat tgaacaatag gggattttat ttttatttag
    14881 ttacatattt tgcagtctat tattatctag gattttttat agatgcttga gctgctgcaa
    14941 gtctagcaat tggcactcta aatggtgaac aagatacata gttaagtcct acactataac
    15001 agaactctac tgaagatggt tctcctccat gctctccaca tattcctagt tttaattctg
    15061 gtttaacttc tctacctaat ttagctccca tcttaactaa cttaccaaca ccattttggt
    15121 ctagtacttg gaatgggtct ttttctaata tttcttcatc tacatattga cctaagaatt
    15181 taccagcatc atctcttgaa tatccaaatg ccatttgagt taagtcatta gtaccaaaac
    15241 tgaagaagtc tgcttcttgt gctatttcat cagcagttaa acaagctcta ggtatttcta
    15301 tcatagtacc tacagtataa ggaacttcta taccttcttc ttttattata gcgtcaacag
    15361 tttctactat catttttctt attattttta actcatttac ttctccaact aatggaacca
    15421 ttatctctgg agaaacttta acacctgctt tgattgcttc tatagcacct tgtattatag
    15481 ctttagattg cattacacat atttcaggat aagttattgc aagtctacaa cctctgtgtc
    15541 caagcattgg gttaaactca tctaaatcaa ctattctctt cttaatatca cttactttta
    15601 ttcccataga ttttgaaagt tcttctatag tctcatcatc atgaggcaaa aattcatgta
    15661 gtggtggatc tagtaatctt atattaactg gttttccatc cataacttta aataactcta
    15721 caaaatcttc tctttgcatt ggtaatattt tttcaagagc tttttctctt tgttctactg
    15781 tattagataa tatcatttct ctaactgctg gcaatctagc ttcatcaaag aacatatgct
    15841 cagttctaca tagacctata ccttctgcac caaactctat agctgcacga gcatctctag
    15901 gattatctgc attagttcta accatcatac atttgtgttt gtctacccag ttcattaatt
    15961 tttcaaaatt tccagtcaaa gatacttctg ttttcttaac atctcctagg tacacatttc
    16021 ctgtagaacc atctatagaa atgtattcac cttcttttat aactacatca tcaagtgctc
    16081 ttatttcttt attaaactca tcaactttta tttctccaca tccagcaaca caacatttac
    16141 ccatacctct agcaacaact gctgcatgtg atgtcattcc acctctagct gtaaggattc
    16201 cttctgcttt aaccattcct tctatatctt ctggagatgt ctctagtcta actaaaacta
    16261 ctttttctcc tttttcattt gcttttacta catcatcagc attgaaataa actttaccac
    16321 ttgctgcacc tggtgatgct ggtaaacctt ttgctataac cttggcttct tttaatgcct
    16381 tatcttcaaa ttttggatgt aataattggt ctaattgatt aggctcaatt ctcatgatag
    16441 cttctttttc atcaattatt ccagcttcaa ctaaatcaac cgctacattt attgctgctg
    16501 ctgctgttct cttaccattt ctagtttgta gtatatataa tctttcattt tctatagtaa
    16561 actctatatc ttgcatatct ttataatgac cttcaagtat atgagtaatt tttacaaatt
    16621 catcgaatac agctggcatt acatctgcaa gagttgatat attttgagga gttcttatac
    16681 ctgcaacaac gtcttcacct tgtgcattca ttaaaaattc accaaataat ttattttctc
    16741 ctgtagctgg atttctagta aatgcaacac ctgtaccact agtttctccc atattaccaa
    16801 ataccattga ttgaatattt actgctgttc ctaagttatg agctatatca ttcaatttac
    16861 ggtatacgat tgcacgagga ttattccatg atctaaatac agcctctatt gcaagcatta
    16921 attgctcttt tgggtcttgt gggaaatcat tttttatttc ttttttataa attgctttaa
    16981 attcttttac tatttctttt aaatcatcag ttgtaagttc tgtatcaact gtgtatccct
    17041 ttgcttcttt tactctgtct aatacgtttt caaatttata ttttggaact tccatagcaa
    17101 catcagaaaa catttgtatg aatcttctgt aactatcata tgcaaatctt tcattttgtg
    17161 tagcctctgc taagccaaca acactattgt cattaagacc taggttaaga attgtatcca
    17221 tcattcctgg catagagaat actgcaccag aacgaactga tactaataat ggattcttat
    17281 tgcatcctaa tgttttattt aagtcttttt ctaaagcagc taaatgctct tctatttctt
    17341 ttattatctc tgctcttata ctttcattgt taacataata atcattacat gcttctgttg
    17401 ttatagtgaa tccaggaggg acaggtaagc ctatcttagt catttctgct aaattggcac
    17461 ctttacctcc tagtaaagat ttcatgtctt tgcttccttc accaaaacta taaacatact
    17521 tagtttccat tgttaaatcc cccattaatc tttttatttt aatttatatc atttttatta
    17581 atagacatta ttaaatttct atttctttgt atatctttag accattttct ttcattatgt
    17641 ctaaaattat acctgctgtt tcttctattg ccttatttga aacgtttatt actgggcatc
    17701 caattctttt cattatctct tctgaataat ctaactcctg aagtattctt tctaaattag
    17761 cgtagtttgc tttactggac aatcctaaag cttttaatct ttgggttctt atctcattca
    17821 gcttttcagg tgaattagta agacctatta tttttttagt atctatctca aacacttctt
    17881 taggaattgg tatttctgga actaatggaa cattagctac ttttatattt ttattagcta
    17941 aatacatact caaaggagtt ttagatgttc ttgatattcc cactaaaatt atgtctgctt
    18001 gaagaacacc tctagggtct tttccatcat catatttcac tgcaaattct atagcttcaa
    18061 ctctcttaaa gtaagattca tctaattttc taattatacc agcttctctt ttaggttttc
    18121 ttcctgttct cttgcttatt tctcttagta tatctgtcat taaatctata caactaagac
    18181 cttctctttc gcaatatctt tcaacaatac tgcataattc ttcatcaact aatgtatata
    18241 caattaaagc gttttcagct tttgcattct ccaaagtttc aagtagtact cctttattaa
    18301 tcatgtatgg aaatcttttt atttcataag tttcattcac ataaaactga gacatacatg
    18361 cctttgcaac ttgttgtgca gtctccccta ctgaatctga tactgcatat ataattaggt
    18421 ttttcaccag catacctcct taattgtcaa ctatttctaa gaatagctta gttatgttgg
    18481 tttttgatat tctcccaaca actctcatat gatttttgtc ttctccctta tattcaacaa
    18541 tcggaatcga atctacttca tgttctatta tttttcttga agctaataaa acacttgctc
    18601 ctttagttaa agtgactaca tttggagttc ttgtcattat catacctatt ggcattttat
    18661 ttatatcaga acctcctata gttgctttta gcaagtcttt tctggaaaca actccacata
    18721 actcttcatt ctcatctata ataactatac tccctacatc agataaaaac atagtgacta
    18781 ttacatcata tatactttca tccttcttag ctagtacagg catgctcatt atatcctcta
    18841 ctgttttttc ttttacactt ttaccaatta aatttatttc actaactcct gaataaaaat
    18901 atccaacttt tggtcttgcg tctaatattc ctgtcattgt taatatagct aaatcagatc
    18961 taagtgtagc acgagtcaca ttcaagctgg aggcaatact ttcactcgtt attggttcat
    19021 tttccttaac tatatctata atttttagtt gtctttcatt aagctgaata ataatcacct
    19081 tctttcattg tgctatactt tttatctttt cgcatcttat atagtatatt atataactat
    19141 atataccatt ctgtaaacag attttttatt gaatacgata tattttttac taagttctct
    19201 cgcttaaaga tatttattat atattaatgg gggatattcc tttaattaat agaactatat
    19261 tagtattaca gttaatatta taatacataa ttggtcattt taattcattt tatattaaaa
    19321 tttttaataa tattgtttca atgtgaatat aaaattgcag aactaattca aaataatttt
    19381 tacgactaca aataacctat aatatacttt taaagaaagt atcctaacaa gaatatataa
    19441 aaatttaaaa gggttgattt aaaaataacc atcaaccctt tttactaata tctatttata
    19501 ttaccatcaa atcttaccca aatatttttt cttgcaattg tcttaaatga tataaagtag
    19561 tatctttttc tatctctacc atatcgtaag ttataacttc acctttttta atatctttta
    19621 caactttagt ctttttactt atcaaaccaa taggtacagc attcatagcc tttgctacat
    19681 cgtatttttc tatagtacca tagcatgtat atgctccata gccatccata aactcacctt
    19741 ttttcaaatc tttcttagct atagtcataa cttctgctac tagtccagct ttaggtacta
    19801 ttgtagcctt atggtctaag actgccatag ctgctgataa aggagtctct aaactacata
    19861 aatgatatgg tctatataat atgtaattag gtccatcacc catacttaaa tattttagtt
    19921 catcattaac tgcttttaat ttatgggcta ctactacgaa aactccaggt gctataccat
    19981 ttacaaaatc aactacacca taattatcta atactccacc ttcgcttttt aatgcatatt
    20041 tcttaggtac ttcattaact gtagcttcaa taccatgacc acctcttaca tcaggcacaa
    20101 atccagttgc attacacatt aaagccattt ctaccattgt tttagttcct tctttaaaag
    20161 aagctatcat atgaggtgaa gctccttttc tttctgcttc ttctctaact gtatcaggat
    20221 tacaatctaa atctaattta ttatttttac ctttacctat cactctaact tcaaacccca
    20281 ttgcatctgc aaaatcaaat aattccatta ctgcacctgg ctcatctcct gcagaacctg
    20341 taaatattac tccagcatca tctgctaatt ttttaagtat tggtccaaca acacaatcag
    20401 tttcagcatt taacataact atatgttttt tatttaaaat agagtttaat gctatctctg
    20461 caccaattgc aatacctcca gttgcatcta taactacatc tattgactta gttttacaag
    20521 ccacaagaaa atcattacat gtcaatactt ttccttcttg taattttatt tctgcatcct
    20581 tttcatttgt tacattatct ataatttctt catctattcc tgcatcagta aaagctttat
    20641 gagccaactc caaatttata tctacaacaa tagaagcttc aatacctttc atatttgcca
    20701 tttggcttac cattccattt cccatatgcc cagctcctat aagagctact tttatttttt
    20761 taccttcctc ttccaatttt gcaagttttc tgtttaaatc taacatacca attcctcctg
    20821 ttataataat tttattttca aattaagaaa tagttattaa atctccaata gttatatctg
    20881 gatttcctat ttccacatgt atttctcctg gtaaatttac ttcacatttt ccatcaaatt
    20941 tgattgtaca atgcccaatt tctctaagag tcttctgagc aatatcacca actgaaacta
    21001 tacaataatc tctattccct atagtaagtg tatctcctat ttcaacatca ctttttaacg
    21061 ttgctattga atgaattaca gatatatcct ttaagtcgtc atcattaatg gtttcatcat
    21121 atataataag acaattgctt tcatccatta actctttaac taaactgcct atcccactta
    21181 tcttaacttc atatttcatg acattcctcc tgataattta tatttatatt gagttaatta
    21241 aaagaaacat ttattttttc ttttataaat tttatagctt gtacaatact ctcttcatct
    21301 ttaaattttt gctctgcact atatatatct aatccttcta tttcttcaat ctctttaaat
    21361 ttagccatta ctgtacgtcc ttttaaaatt tctccaaact ttataaaccc atctgaatct
    21421 gaagatataa tggctatact tcctttagaa aaatagcttt tctttttgcc tgtagacact
    21481 atatcaccct tttttttagc tctatttaaa gttttaaaat acaatttatt ctgcttcatt
    21541 ataaatatag tatttaaaat ataagcaaca aataaaatca ctaataattt catatttata
    21601 caccttccaa aaatcacatt atttttaaat ctgtataaca actataaatg ctgtacaata
    21661 gttatataag gtttttaagt aacacctttt taaattctaa aaaagttcta catttactta
    21721 tcttattcgt taattttaca tcttggccta tctttaaaac ttcttcataa atttttacta
    21781 ataagtctga gtctatctta ttctcacatg gaaacattgt cataagaatt atttttatac
    21841 ttctatttgc atattctatt ggttcttcta aaattccaac tgccattaaa aacttatctg
    21901 atttttcatt tacagaatga ggaaataata aacctttatc aaatactgtg ggagattttt
    21961 cttctctatt gactatattt tttctaaact tatcatcaat gcatcctaaa tccataagat
    22021 ggtccatcat tttttctaat gaagcaagta tagtcttttc atttaatatc ataaatttat
    22081 cttcatctaa caaattattt attagtaaat ttgtagaatt agtagtgtca atattccctt
    22141 ctcttaggca caatactgtt tttaattgtt ctcttagttt gttttcatca aatattgtat
    22201 ttatttttaa tactattgta tttatatttg atgtgtctaa atcaatagtt gtaaaaacta
    22261 tatcatattc atctaagtta atatttctca aattaaaact tgaaagagta tcaattttag
    22321 gattatttcc tatcagtttt tctactctta ttttcaatag ttttgctgtt cctagacctg
    22381 tactacatat aattgctatt ttttttattg aatagaatct actgctattt ctttctaaat
    22441 atccactgaa atggattgct atatatccaa tctcatcttc tgaaacttct aagttatact
    22501 ttttctctat tacttttcct gctatttgtg ctaatttaaa tggaagtata taattttgct
    22561 taatctcttc taacaaaaca ttttttaact ttatattaaa taatagcctg tttaaagtaa
    22621 aactaagatg atatcctaga cctactctta attcatcctc atttataaaa atacccatat
    22681 cgacatatat ttgattaaat atattttcca tcaactcatc aatgttttta ctaattctac
    22741 tactatttaa agcacattct gatgctggtg catttccact aactaacggt atagttaaat
    22801 aaataatttc ttcatcattt aaagtacatt caaacattcc tgatattata tgttttattt
    22861 ccctagcaat aaagtattca tcatatgatt taagtaaatc aaactttaca tcataatctt
    22921 ttactttctt ttcttctttt actctataga ctaaaactac tatgtagtta ataacttctc
    22981 ttaacatttc atctgtaaca taaaaattat tttttttaaa taaagtcaat agtttttctt
    23041 ctaaatttat gatttcttct tttttgattc ctttaaaata ctttgaatta ctgaaatctt
    23101 tccttgagtt tttatacaac tgatttaata taaacagtct aatatcaagt tcatttccat
    23161 ttaatttcat acctgtattc tgttttttaa tcaagtttaa attatatgaa gccaaaactt
    23221 tttctacata ctggaaatca tttactaaag tagttcttcc tacacacatc tcatcagcta
    23281 aactatcaag tgtaataaat ccatctagtt ctatcaagta atttagtata aactctactc
    23341 tatcttcttt tgaatttaat gaaaatacat tttttctatt catatcaata atcttattga
    23401 gtatctgctc atcttttatt tctagtttat agccttgacc ctttatatat ttgattactg
    23461 ctatatcatt acctaaatct ttattcaatt gttttatata cgtccttaca cttctagttg
    23521 tgacattcaa ttcatttgct attgagttaa tactcacaaa ctctttttta ctacacataa
    23581 tcaatatctt aaatatcttg cggtcaatag aaggaatttt ctcagttaca ctcattttgt
    23641 atctcctcct tgatttcatt ctagtacaaa tttgaaacca aattaataca taaaatttca
    23701 ctttaataaa ggaaaattta gtattaatct aactcgataa ttacagctaa ttaatagtat
    23761 aaaagctagt ctttcattaa caaaaaatct tgactagctt aaatttatat ttacaatttt
    23821 attaattaat tccaagcctt atattgacat taaatattat ctcaatgcaa atactgcaac
    23881 tccatatgca ataataacag ctattggacc agttattaaa cgagagtaaa ataaagctgg
    23941 aacaccatac tctacagttt ctggttctgc ttctcccaaa cttaatccta ctggtacaaa
    24001 atcacatcca gcttgcccat ttattgcaaa taaagctgga agtgctaaat atgctggtat
    24061 tgcaccaaga ccaatttgtg tacctacaag agtccctact acttgagcta taactgcacc
    24121 aggtcctagt attggtgata agaatggaag tgtgcaaatt actgatataa tcaataatcc
    24181 accaatatta cctgcaagtg gagatataac tcttgcaatc cagtctccca gtccagatgc
    24241 taaaataatt cccataagca tacttacaaa tgccataaat ggtattacgt tattcattac
    24301 catttggata gtatctcgac ctgcatcata aaattttgat actacttgcc ctgccccttg
    24361 accaatagaa agtattatgt tctttttagc atattcacct tgtgcttttg acttaacctc
    24421 atcataatta tcaggctttc taatagaact cttattttcg cttttatttt cttgagattt
    24481 ttgtggcata tcttccccat ctacaacact tatacagtta ggattaacat ctgatacata
    24541 atattcttct gttataaatt ttgctaaagg acctgttttt ccaactggat ttacatttat
    24601 tgtaggtatt tttttctttg gataaactcc acatctagca gttccaccac aatcaataat
    24661 aactactgct acttcttcat cagaaactcc agttttaaat ccatctacaa tttcacaacc
    24721 cagtagttct tttattttta atgttacttc atggatacca ccagcagtca ttgacagtat
    24781 cttatttcta ttcccttctt tttttatata caaaggacct ccccagcctg aagttccctt
    24841 ttctattttt aatattttat tctcctccat tttatctacc tcctaagcct tatttgttgc
    24901 tctatttttt cttgttagaa aagcatacat tttttctgtt actatacctc ttataaaaat
    24961 tacaattaga ccaactataa aataccacaa tgccaatgta gtagtttctt tacctaaagt
    25021 agtcaaacct gcagatattc ccaaccatac aaaaagttct cctgcatttg catgtggaaa
    25081 aagacctgta attggatgta caaatgaaac tgctgcatca taaaatcctg ctttgtgctc
    25141 ttcttttaaa aatctaccaa atgtatagca cataggattt cccaagaaaa atactgatac
    25201 taatggaagt agaatatatc ttgtaaaaga gttctttcct agcttagaag cccaattatt
    25261 aactctttcc tcaccaatta tcttaatcaa agagttaata gctaccatta atacaattag
    25321 cgttggaaga gtacctgaaa caagacctat aaattgttct ccccctttct gaaacaaacc
    25381 gataaaccac tctgcacctt tacttagacc tgtaataacg ttttccataa aattataacc
    25441 tccctataat atctttaaat ttttaatttt actgcttacc ataaatttta caaattacat
    25501 cttttttctg tactatatgc taatattgta aatgtttttt tattaatatc attttaatat
    25561 aatatttttg ataaataaat acatcttttt tcaattttat aaaggaaaaa attgtactaa
    25621 t
//
LOCUS       DAEMIK010000045        25394 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEMIK010000045 DAEMIK010000000
VERSION     DAEMIK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.045"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(224..1072)
                     /locus_tag="KPK87_001591"
     CDS             complement(224..1072)
                     /locus_tag="KPK87_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2537747.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(1108..1578)
                     /gene="trmL"
                     /locus_tag="KPK87_001592"
     CDS             complement(1108..1578)
                     /gene="trmL"
                     /locus_tag="KPK87_001592"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG2537748.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(1772..2989)
                     /gene="nth"
                     /locus_tag="KPK87_001593"
     CDS             complement(1772..2989)
                     /gene="nth"
                     /locus_tag="KPK87_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG2537749.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINK
                     KGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKG
                     ETEKKTKKSSSNKATQKTSKETKSKFKIKNESANEKDVNKILDELEKLYPDAKCELNY
                     GTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKS
                     KSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVF
                     RVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIK
                     EDCNYYKELNETK"
     gene            complement(3187..5424)
                     /locus_tag="KPK87_001594"
     CDS             complement(3187..5424)
                     /locus_tag="KPK87_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2537750.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(5530..6444)
                     /gene="queG"
                     /locus_tag="KPK87_001595"
     CDS             complement(5530..6444)
                     /gene="queG"
                     /locus_tag="KPK87_001595"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG2537751.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7284..7688
                     /locus_tag="KPK87_001596"
     CDS             7284..7688
                     /locus_tag="KPK87_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2537752.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(7945..8940)
                     /locus_tag="KPK87_001597"
     CDS             complement(7945..8940)
                     /locus_tag="KPK87_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2537753.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(9224..10060)
                     /locus_tag="KPK87_001598"
     CDS             complement(9224..10060)
                     /locus_tag="KPK87_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG2537754.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            complement(10548..11111)
                     /locus_tag="KPK87_001599"
     CDS             complement(10548..11111)
                     /locus_tag="KPK87_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537755.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(11209..12138)
                     /gene="glsA"
                     /locus_tag="KPK87_001600"
     CDS             complement(11209..12138)
                     /gene="glsA"
                     /locus_tag="KPK87_001600"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG2537756.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(13073..14740)
                     /locus_tag="KPK87_001601"
     CDS             complement(13073..14740)
                     /locus_tag="KPK87_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG2537757.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMEIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(14814..15569)
                     /locus_tag="KPK87_001602"
     CDS             complement(14814..15569)
                     /locus_tag="KPK87_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG2537758.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KDRISKLGLSIGIHLPMELNTCENIEYIRDSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(15579..16115)
                     /gene="lepB"
                     /locus_tag="KPK87_001603"
     CDS             complement(15579..16115)
                     /gene="lepB"
                     /locus_tag="KPK87_001603"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2537759.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(16281..16817)
                     /gene="lepB"
                     /locus_tag="KPK87_001604"
     CDS             complement(16281..16817)
                     /gene="lepB"
                     /locus_tag="KPK87_001604"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2537760.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(16917..18227)
                     /locus_tag="KPK87_001605"
     CDS             complement(16917..18227)
                     /locus_tag="KPK87_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG2537761.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTRKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(18306..19022)
                     /locus_tag="KPK87_001606"
     CDS             complement(18306..19022)
                     /locus_tag="KPK87_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG2537762.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            19267..20538
                     /gene="sleC"
                     /locus_tag="KPK87_001607"
     CDS             19267..20538
                     /gene="sleC"
                     /locus_tag="KPK87_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG2537763.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(20964..21620)
                     /locus_tag="KPK87_001608"
     CDS             complement(20964..21620)
                     /locus_tag="KPK87_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537764.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(22893..23303)
                     /locus_tag="KPK87_001609"
     CDS             complement(22893..23303)
                     /locus_tag="KPK87_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537765.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(23519..24901)
                     /locus_tag="KPK87_001610"
     CDS             complement(23519..24901)
                     /locus_tag="KPK87_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2537766.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVEVGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(24912..25271)
                     /locus_tag="KPK87_001611"
     CDS             complement(24912..25271)
                     /locus_tag="KPK87_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2537767.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
BASE COUNT         8243 a         4693 c         2372 g        10086 t
ORIGIN      
        1 aattttttta tatacaattt acaagtacat tttgaaattt ttttaataaa tcttactata
       61 aaaaagtgtg caatagattc ttatctattt acacacttcc ctacaaacta aatctaatat
      121 atcaacactt aatttcatcc aaaataaatc caatttaaca tctcaataat taatattcta
      181 tcctaattta ataaaaaaat aaataatgtc atccaaaaat atattattta ttttttaagc
      241 atatgatacc agtaacacca ggaccagcat gtgcacctat acacacacca atttggaaaa
      301 atctaatttc tttaggtctt aattcatctt gcataacctt agataattgt tctttttcct
      361 taaaatcatc actatatcct atatatacaa tttggtctga taaatcatca ccacaatttg
      421 attttatcaa ttccatcatc ttagaaatta cattcttctt tcctcttact tgagatattt
      481 gcgaaactaa tccatctctt atttcaagaa tcggttttat atttaatata cttcctattg
      541 ctgccttagt agatgatatt ctacctccct tttgtaaata ttccaatgta tctactgaaa
      601 atataacaaa agccttctct tttaattcat ccaaaacagg caatatttca gcaacagact
      661 ttccttcact tgcaagttct cctgctttta ttacaagtaa accagttccc atacaaagca
      721 agttagagtc atatgtatat attttaccat ctacatcatt ctttgccatt acagcacttt
      781 gatacgtacc tgtagctgtg gcagaccctg ctatataaag tatctctcta tcctcatttg
      841 tatatttctc aaaaatttcc ttaaattggg cataagtagc ttgagaagtt tttggatata
      901 cattttcttc tctaagcttt ttataaaatt catctccaga tatatcaatt ctatccttgt
      961 attctctttc ctccagtata actgttaatg aaactatatc tatatcatat ttatctaaca
     1021 aatcttttgg cacgtccgat aaactgtcac agattatctt tagatttttc attaagtatg
     1081 ctcctttctc atttaggctt gttgaaacta cctcatattt gggtatccta tttgtcttaa
     1141 agcctcgtac actacaatag caactgaatt tgataaattt aatgacctag ctttttcaac
     1201 atccaacata ggcactctaa tacaagtatc catatttgct ttcaacaaag gttctggtaa
     1261 tccctttgtt tctttcccaa atactataaa acaattttct tcatatttca catctgaatg
     1321 tgactgttta gcctttgttg tagaaaaaaa gtatttacta tttggatact tttcttgtaa
     1381 ttcttcaaag ctatcgtaat attgtatatc agctatatcc caatagtcaa gcccacatct
     1441 ttttaggtgt ttatcatcca atgaaaaacc cagtggtttt actaaatgta aagttgaacc
     1501 tgttgctgca caggttctta ttatatttcc tgtattttga ggtatttctg gttctactaa
     1561 tactatattt aatgacattt taatcctccc taagtatcgc ttttatttat caaattttat
     1621 tttataaaca gaataacaat aatattatac tattatataa aataatgtta ataataattt
     1681 ttatttcatg tatataatag ttttttatct aacattttca caagtattat tatatcagaa
     1741 aatccctctt gtacaactta ctttctaaaa gttatttcgt ttcattaagt tctttataat
     1801 aattacaatc ttcttttatt gggcaacttg cacattctgg atttctagct ttacacattc
     1861 ttcttccatg aaaaattaat acatgatgtg agtgtgacca tctttctttt ggtatagctt
     1921 ccataagtgc aaattctgtt ttttgaggat ttggctcgtc cactattcct attctattgc
     1981 taactctaaa tacatgagta tctacagcta ttgctggatg attaaaagcg ttactaagta
     2041 caacccctgc cgtttttctt ccaacaccag gtaattttat aagcttttct aagctatcag
     2101 gtacttcacc atcatataat tcacatagct gttcagatgt atcctttatt ttttgagact
     2161 tgcttttgta aagaccacaa ctctttatct ctttactaat ttcttcaatg ctcaaattag
     2221 caaagtctct agctgtgttg tactttttaa acaactctga agtaacttta ttcactctta
     2281 catcagtaca ttgtgctgat aaaatagttg ctattaaaag ttcaaaggct gtcccataat
     2341 ttaactcaca tttagcatct gggtacagtt tttctaactc gtctaaaatc ttatttacgt
     2401 ctttttcatt tgctgactca tttttaattt taaacttact tttagtttct ttacttgttt
     2461 tttgtgtagc cttattagaa ctactcttct ttgttttttt ctccgtttca ccttttttag
     2521 tacttttttt acttactttg atactttttt tatcttcttc aatatccata aaattctcta
     2581 tatctttctc atcaattatc tcatcttttt taccaatcat tttatctatt tctttttcat
     2641 ttatctcttc cttctcaatt atatctttct tattcattcc ttttttattt atctcttcct
     2701 tctcaattat atctttctta tccatttctt ttttatttat ctctttcttc tcaattatat
     2761 ctttcttatc tattcctttt ttatttatct ctttcttctc aattatatct ttcttatcta
     2821 ttcctttttt atttatctct tccttctcaa ttatatcttt cttatctatt ccttttttat
     2881 ttatctcttc cttctcaatt atatttttct tatccattcc ttttttattt atctcttctt
     2941 ccaaaattac atctttttca tttatctctt ttagatttac atttttcata attatccccc
     3001 ttcaaaatac caatttataa aacgctattt ataaatagtt taatcatatc aacaaaaaaa
     3061 taaacatcaa tatataaaac atacaaaata agaatttaat ttatatatat ttacaaaaaa
     3121 agcaaaaacc tattaaagtt cttgcttttt attatacata aatacccatt aaattctata
     3181 ataaacttat tcttttgaca cagtttgtat cttgctgaaa gatattaatt tttcttttac
     3241 caatttcttt atttcttcaa atttaacatc tgtcaatatc tctatagcct cttctactct
     3301 ttcaacagta tatatcttaa attttccatc tcgcactgca tcattaactt catctgataa
     3361 aacgagattt ctttgatttt ttctaggaat tatgacgcct tgctcaccat ttaacccttt
     3421 agttttacat atagaataaa atccttctat cttttcactt ataccaccaa ctacttgtat
     3481 atcacccttt tgatttatag aacctgtaac agcaatattt tgtttaattg atactccact
     3541 aagagatgat aatagtacat ataattctgc agaagatgca ctatctccat ctattccacc
     3601 ataattttgt tcaaaacaaa catatgcatt tagtgacaat agtaaatcct gtgcaaaatt
     3661 ctcagaaaga tagccaccta atataagtac tcctttgtta tgtatagagc cactcatatt
     3721 tacttctctt tctatattta ctatgccttt acttcctggg ctagtggtcg ctgtaatcct
     3781 tgaagctcta ccaaaagagt attctcctgt acttaagact gacaaaccat ttatcactcc
     3841 tacttttctt ccctctgttt ctattagagt aaatccattt tcaattgatt catccatatg
     3901 tgtttccact ctattgattc tttttctctt ttcacttatt gcttttttaa catcacactg
     3961 ctttgtatac tccgaatttc taaactctgc ataagcactt ccttctatta caacttccat
     4021 tattttatta aacttagttg ataatttttc taaatcacca gataatctag tgctaaattt
     4081 aataacctct tcaactgctg atttagtaaa atgccttaaa ttattcttat cacattggta
     4141 tgctataaat cttgctattc cttcttcatt atcttcattt ttatccattt cactatcaaa
     4201 gtctacaaat actttaaaat atttttcaaa atcttcatca tatctatata aaatatcata
     4261 tatatagtgg cttccaatca gtactaattt aacatccaat ggcatatttt ctggtttaat
     4321 tgctgtttgt gtttccaata ttactttttt agtctgcaaa gtctttttaa gaatttccca
     4381 agacattgta tattttagta attggtctgc ataaagtact aaataaccac cattagccct
     4441 atgaaatgct cctgataata gttttgtgaa atcagtcttt atatttccat ttgcataatc
     4501 atactctgcc tttccaaata gatttgctgg acttgggttt atctctacta caacaggtgc
     4561 tgattcttta ttctttgagc ttccattgtc tacaaatagg ttaattttat atttaataaa
     4621 atgctcttta tcatacttat cttttaactc ctcttcatct aaatagaata aatacatata
     4681 ttctattata tctttcttta tattttccag ataagtttga atcttatcat aattttcata
     4741 tttttcacac aatctcttga tatgaggttc tactacaagt ttagctatct cttcttcaag
     4801 ctctaacact gctttttcag ctatttcttc taagtttcta attttatata cgacctgtat
     4861 cgccatattt tctagttctc ttttagcttt gtaaaactct tcatcagact catcactttc
     4921 atcttcctct tctttcaaag ggataaaaac tattcccatt ttactctgtt ttaatttaaa
     4981 tcctttttct tctccatatt ttttaatttg cttcaaaagc atatcttttt ctatttcata
     5041 ttcatccaga agtttatttt tttctatttc aaaattttca ctttcaaacg catttttaaa
     5101 atcttctaaa agactttcaa ttaatttctc catatctttc ttaagttctc ttccaaaccc
     5161 cttttctaat tcaactataa taggctctct agggttttca aaattatata tataacacca
     5221 atctttatgc gtattttttt tggatgcata ctcatttaaa acctttaagg cataagtact
     5281 tttaccagtt ccaggttcac cagctatata tatgttgtac gctggattat ttattttcaa
     5341 ccctaattcc atagcttcta cagctctttc ttgacccaat atataatcta gtggatttat
     5401 ttcagatgta ttattatatt tcaatattcc cccacctccc catatttaac tttaatgata
     5461 taatatgact tttacacaac aagtgttaaa gtaaatacac attaaagtat atgaaacaga
     5521 ctcttattat tagtcatcat acttatctaa aattatttcc atatttctct ttattatatt
     5581 tctacctctc cagctaaaag ctctatctcc atatctcctt ttaaattctt tatttgaaat
     5641 attttgtatc tcatcataat ccaaataatc tattatattt tctttaaatt ccaaaatatg
     5701 agttgttggt atttcaacat tatgtggaca tacttcttga caaatatcgc atccaaatac
     5761 ctttttgcca ttctttaaaa caactttttc tttctctgac aaatctcctt ttttttgagt
     5821 tatatatgat aaacattttt tggggttcat atcataattt cccaatatag catttcccgg
     5881 acaatatttt acacacttac cacatttaaa acaacttttt tctagtggtt tatcttcttc
     5941 aaatttataa ttatttacta tataccctat aaatacgtaa gaaccatatt catcagttat
     6001 gatattatta tttattccaa aatatcctat tcctgataaa taagctaaat acctgtcaac
     6061 taatggtcca ttatcagcaa ataacttgta tttaaaatct gttactgttt tagaaatata
     6121 atcacaaatt tgttgtaaaa agtcctttac tacaatgtga taatcctttc catgacaata
     6181 ttttgataaa tttgaatttt tattcttttc ttgtctattt atatgatatg gaaatgcaca
     6241 gactattata gagttagcat ccttcattat atgtcttggg ttaattctgc tctcaattat
     6301 tggttcttcc ataccagtta aataaccttt ttgttgtctg ttcttaagta tcttttctaa
     6361 attatcatat ttatctaccc ctgctactcc tacacagttt aatcctatta aattacaaaa
     6421 tgcttttaat tccttattat ccataaaatt attccttttt aatctaattg actttttaaa
     6481 tttatttaat ttttttagtc tttttaacct tttctagcta ctaactcttt taaatactta
     6541 atctttttag ctattcaatt ttcttacctg cttaactctt ttagatactt cgtcttttta
     6601 tctatttaat ttttttacct actaactctt ttaggtactt cgtcttttta cctatttaat
     6661 tttttaccta ctaactcttt tagatacttc atctttttac ctatttagtt ttttacctac
     6721 taactctttt agatacttca tcttttttat ttacttattt aactttttta cctactaact
     6781 cttttagata cttagccttt ttcatttact tatttaactt ttttacctac ttaactcttt
     6841 tagatactta gcctttttaa tctattcaac cttttttacc cacttaacct tcttaccttt
     6901 taatataaac tctaaaactt caaatatcat tagttatatt ttgacattct ttctatagtt
     6961 aagcaaaaag attgattttt tcttataatt aatatcccat ttacattgcc atttattaaa
     7021 tctttacttg acttatatgg atttcttttt actctcttta ctaaactata cttattaata
     7081 ttatgaaggt cttataatag tctaatatga atatgtccat catatatcaa attttaaaat
     7141 ttgtaagcaa aatacttatc ttatattacc acatctaaag tggaaaattt tacaaacaag
     7201 ctctgtaaaa acactttaaa taacaatttg cttttaaatc atatacagat tataataaat
     7261 actgtataag gatggtgaaa aatttgatta aaataacaat tccaggtaga cctattagta
     7321 aatctaattt taagcttcat aacataaatg gtcaagcatg gatgccttca aagggaaaat
     7381 attctaagta tcttgcatac gaaaacatga ttgcaggttt tataaaccaa cagtatcaag
     7441 gggaaactgt tgaagaaaat ctaataacag tcttgaaatt attcttccca aataagagaa
     7501 tgggggattt acataattat ccaaaaagta tttgtgatgg tattgaaaaa agtggaataa
     7561 taaaaaatga taaacaactt aagccagtac ttttatttga ttttatagat aaagataacc
     7621 ctagggttga aattgaactt tatccagtat ctaaatacga tatatcctat aatatttttg
     7681 aaaaataaag attttatgtg ttattcgaca ttatattgat actccagtat ttgaaactat
     7741 actaatacaa caataataat tgtgttatgt ctatactcaa acaaatgttt ttaaaaagca
     7801 ttaagtaaat atttaataag tattaaacaa atattaaatt tatataatag ctataactat
     7861 gtaaatttaa tatttgtata taagaccaca tctaataaaa agaaatcgga gtaaatttaa
     7921 tacaccgatt tcaataaata aaatctatcc tagcttttca acaacagtct taagcatttc
     7981 tcttatagga agttcatatg gacatctact ctcgcaaagc ccacagtcaa tacactcagt
     8041 aggtttaaca tccataacct catatttttc tttagcccac tcttttaatc catatctagt
     8101 ataataacct tcacataaaa aactcaatgg tatatttatt cctacagcac aaggcataca
     8161 atattcgcat cttctacaga attttttacc taaagaattt cttatttctt caatttcctt
     8221 attatctttt tcatctagca caagattttc aagtacagcg acattttgtc taacctgttc
     8281 aacactctcc atacctggta taactacatc tatgtaatct ttagatagta tatatttaat
     8341 agccagtgtt gcattgtcta aagcacctcc agctaaaggt ttcattacta ttatcccaat
     8401 tcctttttca tgagcttttt taaacacttc atctgcttgt ccttcaacta tattgtaagg
     8461 gaattgtata gtgtcaaatt ttccatcttc tatagctttt tctatagtat ttaaattgtg
     8521 actagttata ccaatatgtt ttatcttacc ttgctccttt gcctctaaaa gggcactata
     8581 agccatttta tctttaaaaa gattatcata ctcctcttct ttaacattat ggaattgata
     8641 taaatctata aaatcagttt taaaattatt taaacttatt tcaacatctc tcttcattga
     8701 atcataatct ctggacatac ttttagtagc taagaaaaat ttatctcttt ttccttcaat
     8761 ggcaattccg attgcttcct cacttattgt ataccctctt gcactgtcaa taaagtttat
     8821 tccttgtttt tctaactcat ttataactaa gttagtatca tcttgagtaa tcctttgtat
     8881 tggaatgccc ccaaagccaa ctctctttat tttcatgtta gtattgccta aatttctcat
     8941 atctttcccc acctaacttt taataatttt ttatataata taaatatgtc atctttaatt
     9001 atacaacaaa ttatgagaat ctttgcaata tatttacata ctattcataa tataattata
     9061 ttttatatca gtctattcat aatataatca tactttatac ccagttaatt tgtatacaat
     9121 ttttgttatt cctatccatt aatttaaatt atcacatttc atgattgtat ctccacccta
     9181 attatgttga gggtgaagta tttcacatat ttttaataaa ggtttattta ctagatagta
     9241 tttcttttat cattttaagc tctttcttgt gaccttcatc atcaaaagga gtttctaaga
     9301 aaaatggtaa atgttttaga ctaggatgtt ccataaaatc aattaatgct tttaatccta
     9361 tctttccttc ccctatagga gcatgtctgt cttttttgtc tccaaatggc atcatactat
     9421 catttagatg gacagttttc aatctttcta tgcctataac cttatcaaat tcttccaaaa
     9481 ccccatctaa atcattcact atatcataac cagctgaaaa aatatgacat gtatcaagac
     9541 acacacctat tttttcttta tgctcaattt tgtctattat actttttaat tgctcaaatt
     9601 taaaacctat ttcagttccc tttcccgaca tagtttcaag aagtatagtt atattttcat
     9661 caccctttat ggcattgttc aatcctttta ctattctatc tataccaaaa tctacaccac
     9721 cacctacatg acttccagga tgaaaacaca tgtattcaat tcctaatgag tccatccttc
     9781 ttatatcctc atctatcacc cttgttgcaa attcgtatac atcatccttt gtaccaccca
     9841 gattcatagt atatggtgca tgtgcaagca gtggtgcaaa attattttct tttctaattt
     9901 cttgaaattt aattatatct ttttcatcaa attctttagc atttcctcct cttggatttc
     9961 tactaaaaaa ttgaaatgta tttgcaccaa tatatacagc tgtctcagcc gcattagcaa
    10021 agccttttgc tattgaaaga tgtgttccta atttaatcat attaatctcc ttttatttgt
    10081 actacgactt gtactcaatt ttaattttga actactccac cttaactaca tttagccaac
    10141 atatgaagat ggagattcct atttgatagt actttttcac taaaaattca taagctctaa
    10201 gggatgttct atcccaacta atctcattcc tcttttaaca tatttctagc attgatttaa
    10261 tttatattaa tttttaattt taatatttac actttaatta tacccatttt aatcaatttc
    10321 aaatattata atcttacata ctttgtatat tatctaagca cttcttagat agtgtataca
    10381 tcttacacta tacatattat ttacaaatac aataggtata aaaaaacaaa aaaatagaga
    10441 ttaaaaatca ttgttttacc cataccaaaa actatttttt gattattaag taaattttga
    10501 tgttcaatct ctaatattat atctttaatt aatccataat taattctcta actaggacat
    10561 tcctcctcaa gagtaggaac tataaccata gctatatcat acttagaacc ttcaacattg
    10621 tgttgtcttt tatttaaaat ttcatctatc tttgacttca tagtgtcact aagttcaact
    10681 aattcatcat gagtaagaga aatatcatga taatcaacta tattttcatt ttctaaaaca
    10741 tcttcctctg ctgccttata aaaaacttca ctcatatttt caaattttga tatgtacaat
    10801 tcttcttttt cttccccatt gtctaaagaa aaattaagta tcctattccc tataaccaca
    10861 ttttttgcac ttggataata gtacttttcc acaatccccg actttatttt ttcctcaact
    10921 aattctaaaa taccaacttt gtataacatt tttatatggt agtttatttt tgcatgaggt
    10981 tcttccaata attgagataa ttgttttgct gacaatggcg atttgtcaaa tgcttttaga
    11041 atatctattc tacgtggatg cgcaatagcc tttatgcact ccaaatctcg taataccaat
    11101 acctctttca ttgacccctc ctaaaaaatt tatttaattc atatttcttt acaatttaat
    11161 tatatattaa attatatgtt gttgtcaaaa aaatcctttc ttaatatttt aaaaaatatt
    11221 taatttgagt ttatttgaca actcctctaa aactttaact cctgcgacgc tgttaccttt
    11281 tttgtcaagc gcaggtccat aaacaccaat ccccatgcgt cccggcacag aagccattat
    11341 accaccccca actccagatt ttgcaggaat gccaacttta atagcaaatt ccccagaagc
    11401 atcatacatt ccacaagtca tcataaatgt cttgacaatc ctagaaacca tttcactcat
    11461 taaatgctca ccattttcta tatctactcc ataatttgca agatttattc caattctcgc
    11521 taaatctaca gaatctatct caattgagca ttgtttaaaa taaaggtcta gaacatcttc
    11581 tacattccct ctgataaatc catcactttt caataagtaa gccatagctc tgtttctatc
    11641 tccagtcatt ttttcagact tatagacatc atagtttatt cctatattat cattttttgc
    11701 taactttcta aagaaactaa gcattctttc ttctttttct tctaaacaac tcccatttat
    11761 aagggaagtg gttactatag cccctgcatt tatcattgga ttacagggtt tttttgtgtc
    11821 attaatttca agtttcatta ttgaattaaa tgggtcccca cttggctcca ttccaacgtt
    11881 tgaaaaaacg tcttcccaat cattatccat aagtgccata gcaagcacaa ttggttttga
    11941 aatactttgt atagtaaact ttgtgctaca gtttccagca ctataaagat tattttcgct
    12001 atctatgata caaattccta aatcatttct tctagcgttt ttcaattctg gtatgtaact
    12061 agcgacttgt ccataattag tatacttctt attagaactt ataatttctt ttaataatgt
    12121 ctcatctaac gtattcatat gcacctctta tattatgtta tatattctat tttagcacaa
    12181 gtttatacta taataaaaca atgttaaaaa aaattatact ttaaaattat tttctacaag
    12241 aatcaattca tttttcaaac aatttaaata gtttaaagag cttaataatt taataattca
    12301 ataatttaac aacctagcaa tttagtaatc taataatcca gtaatttaac aacctaataa
    12361 tttagcaatc taataatcta actatctaat aatccagtaa cttaacaacc tagtaattta
    12421 gtaattcaac aatctaacaa ttcaataatt taacaaccta gtaatctaac tatctaataa
    12481 tccagtaact taacaaccta gtaatctaac tatctaaata atccagtaac ttaacaacct
    12541 agtaatttag taattcaaca acctaataat tcaataattt aacaacctag taatctaact
    12601 atctaaataa tccagtaact taacaaccta gtaatttagt aattcaacaa cctaataatt
    12661 caataattta acaacctagt aatttagcaa tccagcaatc taataatcca gtaatttaaa
    12721 tttttataac accaaaaatt tctaacccaa caccttttac tcttttactc ttttactctt
    12781 ttactctttt actcttttac tcttttactc ttttactctt ttactctttt actcttttac
    12841 tcttttaaaa aataatcaca taaactaaca aaatattaaa caaaaaagta aaatttaaaa
    12901 taaaaagctt gcttcataca aaagtataaa acaagccttt ttatatatca actcaaactt
    12961 taaaatctgt atcaatttaa ttctccactt tacacttaat atacttaaca taaaccaatc
    13021 acactacttg ctatgccatt taatcttcat aaaacaatct atagtacaca cattaatcaa
    13081 ctattttact tcctccaata aattccctaa gaattgaaat acctggtatg tcagaagtat
    13141 cgtccaattc aacaaaatac tgcaaaaact ttagtaatct agtagcttca atatgtgcag
    13201 gattatcttc ctttatctct tccaacaaag gtttcgcaaa tcgttttaaa actgccaatt
    13261 catatacaca agcagagtta aatatagcat ctgcctcctc ttggtacggg aatatattct
    13321 tcttttctcc tcttctaact gatgcccatt gtaaaatagt attatcagca ctatatcctc
    13381 taaaattata gtctctaaca attcttctta tcattctcaa atcagcagta gggattcgat
    13441 tatggtcatc caaatttatt tgagtaaggg cacttatgta tatcttaaat ttatcttcat
    13501 ctggtataga tgatgttaaa ataggattta gcccatgtat accttctaat ataattggct
    13561 gatttgaatc tatctttatc tttttaccag tgtattctct cttaccttct ttaaaattga
    13621 atcttatatc atcaatttct tcaccagcta aaagtttctt taagtctaag ttaaacttct
    13681 ctaaatctat tgcataaatt gattcaaaat cataatttcc atattcatct aaaggagtat
    13741 gttttctatc tataaaataa tcatctaaag aaattgaaat aggatttaaa ttatttactc
    13801 taagatgtat tgataatctg tgagcaaaac ttgtttttcc agatgaagat ggagctgcta
    13861 ttaaaactac tctcttattg ccatccttta tcatatccgc tatttgggat aacttctttt
    13921 catgcaatgc ttcaaatgtc cttattatat ctccatattc attgttttct attattttat
    13981 taagagatac taccttgtca atttccataa gtcttgacca ttcttctgct tcataatata
    14041 cacttgatag cttaggttga ggtacaaatt tcataggaag agtcttatct tcttcactag
    14101 gtcctaaaat tataacgcct ccattataaa gcttaatatc aaatgatttt atataaccag
    14161 ttgatggcaa catataacca taaaaatggt ctatatagtt tctacactta tatactttag
    14221 cactatcata ctctttgtat ttcaaaagct ctgccttttc atacatttca ttattttcaa
    14281 agatagaaat agcttcattt cttgtaactt caattttttc tataacataa tcgttgttta
    14341 ctatctcttt catcttgttt tttatatttt caacatctgc ttctttaagc tttctatcta
    14401 tatgaacttc acaatatagc ccatctgata gtgaatgttc tattgtaact ctgctatccc
    14461 aaaagatttc tctacatgcc attacaaata taaatgacat gactctaaag taaactctct
    14521 ctccatcttc attagtagta tctaaaaaat ttatctcaca atccttctta actctacaat
    14581 ttagctcttt tagtttattg tctacaactg caagtgttat ataaccctta tgctcatcac
    14641 aaaattcttt tgctatatct cccaatctaa ttccagggga attttcttct aatgaatatt
    14701 ccttatcttt tccttctatt tttaagttta tttttttcac ttcaacatct ccttcctttt
    14761 gatgaatata aattttaatt aatgaggcat atcaacaaga taaagagagg tatttaaccc
    14821 tctcctacaa tattattcaa tttacttata gtatcttcaa tgtcttcgta atttatttct
    14881 aatactaaat attctgcatc taatgttaag acctctttaa tcccctcttc agataaaatt
    14941 cctcttccta taccaacatg tgtatcattg ataccatcat tatcacttaa gtgtatcagt
    15001 cttattttat cctttaagtt ctttacataa gcatcctcat caattaaaca gtgtccagta
    15061 tcgtaacaaa aacaaatctt agaattttta atcctcttaa atatatattc aaaatcataa
    15121 gatatatttc caatatttga aaaatcccca ccttttgaat aagtattctc tatacataca
    15181 tatgaatttg tatttaattt atctaaaaaa tccacactat ttcctaaata tttgtctcta
    15241 ttctttctaa ctctatttgt cataacatat cccaaatgaa ggttaaaata ccttaaatca
    15301 aatcctttta gttcaaattc tatcttctca ataaaattta tccatgaatc tcgtatatac
    15361 tctatgtttt cgcaagtatt taattccata ggcaaatgta tccctataga taatcctaac
    15421 ttagaaattc tgtctttata tttacataac tcactacatt cagatatatt gtcaattcca
    15481 atctctaaat gagttatttg ctttatactc tcacaaatgt ttaaagcctc ctcaatatta
    15541 aacaataagg acgctatacc aaccgccata caaatcacct actcaaaaat acctatatct
    15601 gtaaaaggaa atactcttaa aataactttt cctttaatat tttcttcatc tactaatccc
    15661 acttctttat atctactgtc taaactaact tctctattgt ctcccatcgc aaatactttt
    15721 cccttaggaa tcaccatatc aatatcgcct tcagtataat tatcatgtat atatgaaact
    15781 tcatttaata acttaccatt tatataaacc ttagaatcct taatttttag atggtcacca
    15841 ggaacaccta taaccctttt tacaagctct tttttccttc cattctcttg taataaatca
    15901 gtcttaaata tcattatatc tccacatttt ggttctgata acttatatgt catcctgttt
    15961 gctaccaaat aatcatgtga tttaagtgtt gggtacattg attctccatt tactaatgtt
    16021 ggttttataa aacgagttat aataaatgca aaaaaaagtg cagtaataat tatttttatc
    16081 cactctacaa tctcttcttt aatagtttca ttcattcaca aattctcctt tatagttata
    16141 ttatctttat tgataatgtc tcttaaacac tatcttttag ataagtctac taaataaggt
    16201 gttgtctttt ttatatagac aactattgtg tatggtctgg tgatttcagc taatcagcga
    16261 ttgctccaaa ttttatatgt ttagtcaata gttcctatct tagaaaatgg atacaatctg
    16321 actagaacct ttcctaaaat ggtatgctcg tcgaccaatc caacctcatc gtatctacta
    16381 tctaaacttt tttctctatt atctcccatt gcaaataatt ttccttctgg aactacgata
    16441 tcaatatctc catcagtacg attattatgt atataggaag tctcatctaa caacttatca
    16501 tttacatata ccttggagtc ttgtattttt acatggtcac caggaacccc gataactctt
    16561 tttactaaat cttttttctt tccattttcc tctgttaagt cggttttgaa tactattata
    16621 tctttgtatt ttggctctcc taccttgtac gcaattctgt taactatcaa ataatcacgt
    16681 tcaactaatg taggatacat tgattctcct ttgactattg ttggcactat aaaacgagtt
    16741 attgcaaatg ccaaaacaag agctatgaca atcactttta tccattctac aacttctttt
    16801 ttaactgctt cacccactaa aatcgtctcc tctgtgttaa atattaagtc aatctacagc
    16861 aaaataatct accataaacc tacaatataa atattatact tcttttttct aatagtttaa
    16921 aatacttttc taagaccttt aggataatga ttcttaatta caccagaaga ttcttttccc
    16981 caacctaaag gtattccttc aaccgaaacc aaaacccatc ctctactatc attagaattt
    17041 acaacatccc cttttaaata tttaagaatt gattcactct gtacactgaa gttttcaaca
    17101 ttttttacat cttcaacttt taagtaatga gatagtgcat gagaaggctc aaatctattc
    17161 ttctttaaaa cccctaaatg caaaccatat ctcaatactt ttagttttct cgtatctgga
    17221 tatatttcag gaagtaggta caaattttct cctcttaaat aaaatttatt ttttttatta
    17281 ctataaaaat caacctttaa aaatttcttt tcaaactccc tataatcctt tatttcttta
    17341 tctaaatttt ttatttttaa ttctttgaca ttacattctt cttcttctaa tttttttatt
    17401 ttggcaacaa aatgaccttc tccctttatc ttatgaggcc ataatctttc catttcaata
    17461 agttctgcat gtggatattc actgataaac tcatttataa catgctcatt ttcctccctt
    17521 gaaaaagtac aagttgagta aattaaaatg ccatccttct ttaacatttt atatccatct
    17581 cttataatct ctttttggat ggattgacat tctactacct ttgcataact ccaatctgat
    17641 atggcaacct catccttcct aaacatacct tgcccagaac aaggtgcatc tataataacc
    17701 ttatcaaaat acccattaaa cgtctttctt aagctattag aatcggtgtt tgttataata
    17761 caatttctcg ctccaaatcg ctctaaattt tcccctagag ccttaattct actagcatta
    17821 atttcattag aaaccaataa acctgtatcg tttagcttag atagtatata tgtagatttt
    17881 ccaccaggag cagcacacat atcgagaact ttttcaccct ctttaatatc tgcctttggt
    17941 attacactca ttgcagaagg ctcttgcaaa tagtatgctc cagcctcatg cagtggattt
    18001 ctaccaggtc tgtcaatttt atcatcataa taaaaacctt cttctgccca tggtatttga
    18061 ttcaaatcaa acaactccaa atcaaataat tttttcttgt caatttttaa agtatttatt
    18121 ctaagcccag tagtcttact ttcctcataa ctttttataa aatcatcata ttcatctttt
    18181 agtatttctt caatatcttc taaaaattcc tttggtaatt ctgtcaaatc tataatcccc
    18241 cttcaatctg ctttacttgt gtgtcattta ttatacttta attatttatg tctttccttt
    18301 aaattttatt tcttcttctt tttcctaaga ttaccgaaaa acactttttt agagtgattc
    18361 atatatttct caagtttatc aagatttatc ttccacaaaa ttattacata aactataagt
    18421 aatataatag cccatctata ttctatcatc aatatgaaat caaaaactcc atgtaataaa
    18481 attggaatta acactgccat aaacaaatac tcacgtcttt tatttttatt tccctcaaat
    18541 ttatacttag aaatatagta gcccatagta attgcaaaca ttacatgagc tggtatagag
    18601 ataactgccc tattaatacc aacttgcagt gctagatttc tactttcaga aaaaatataa
    18661 accatatttt caattgttgc aaatcctaaa gctaaaaata cagagtaaat aataccatct
    18721 aacttttcag taaagtgttt ttctttaatt agggcaggaa tcagtatcag agcttttaca
    18781 ccttcttcta caagtcctgc aactacaaat gatacatata ttagttctga atacccttca
    18841 aatacattaa acttcatcag taaattttct aaaagtataa ttataacact tagaaaagca
    18901 cctattaaaa aaaatttaat tagatggatt aggggttctc tacaatatct atctttagac
    18961 cttatccaaa acatccctat caatatagga actattgcta ataaaaataa atctagcttc
    19021 atagattatt ttcctttcaa aatttttgat ttatttatga tttatatcat ctacttattt
    19081 ttaacttatt cgtgttttaa atacatacta aaatatacat taaattattt taaattattt
    19141 aaatacaaat atactcaaat actcaaaaaa atttaaattt atatctatat tatttaacaa
    19201 atttcaagct atattcatat cttgaaatat aagggaacta aattcattta aagaaagggt
    19261 gatttgatgc aagatggttt cttaacagta agcataattg atgctactaa caatagacct
    19321 attcaaaatg cagtagtaaa catatattca atgtctaatg gtagccaaag ctcatcaact
    19381 ttataccaaa acctaagaag taatgaatct ggtcaagtga ctggtttagt attaccagca
    19441 ccagatgtag attactctct acaaccatct gatgttagac cttatagcca gtatatagtg
    19501 gaagccatag cagatggata tgagacagtt gtcatagaag gaacacagtt gttagctaca
    19561 attgaagcaa gacaaggagt tcccatgtct cctagaacaa gaagtaagcg ttctttctca
    19621 agacaaagtg agttgatttt tgatatagga gaacatactc tatatggtac ttatcctcct
    19681 aagattccag aaagtaactt aaaaccatta ccacctccta ctggtttcgt tgtattagat
    19741 aaccctgtag tgcctgaatt tatagtagtt catgatggtt tgccagaaga ttcatcagct
    19801 ccaaactatt ggattccatt taaagagtac attaaaaaca tagcgtcatc tgaaatatat
    19861 tcaacttggc ctgaacagac aatctatgca aatgtaatag ctataatatc atttacttta
    19921 aatagagtct ttactgaatg gtatagaaat aaaggatata actttacaat aacttcaaca
    19981 acagcatacg accataagtt tataaataat agaaacttat ttgaaccaat taacgtagtt
    20041 gtagatgcta tctttaatac ttttataaaa agacctccta catcaagaca gccactatta
    20101 gcacaatatt gtgatggaca aaaatcccaa tgtcctgacc aaatgactca atggggcagt
    20161 aaagacttag gtgaccaagg atatgactat gagagtatac taagatattt ctatggagat
    20221 gagatagtat ttgaaagagc acctatagta agtggtgtac cagtgtcatt ccctggtact
    20281 acattacaag ttggttcttc tggacagtat gttagaacca tacaaaatca gttaaatgct
    20341 atatctaatt cttatccagc tgtaccaaaa gttatagagg atggtatcta tggaacagat
    20401 acagaaaacg ctgttaagat attccaagga atctttggtt tacctcaaag cggagtagta
    20461 gattttaaaa catggtatga aatatccaga gtttatgtag ctacaactag aatagcttct
    20521 ttaaatcctt taatttaaag cttgatttag aacataaact caaattattg aataaaatta
    20581 ttaatttaaa ataaaaatgg ctgttatgaa ctaatatata ccataagtat tacttataag
    20641 tattgtagtc ttaacagcca ttttttagtt ataacttgtt tttttagaat cttaaaactt
    20701 taaatggggg attttaagct tcttaaaaac agtgcttagc aacttagatt tctttaaaat
    20761 attatattta tttttaagtt taataaaatg ctattctaac gaaaaaccta ctttttattt
    20821 aaaatctcac ttattattta aaacactatc catcacttag aatattactt cttatataaa
    20881 ttattattta ttataaatac aaataatatt aatttaaaaa gttattccta atttaaataa
    20941 tactattatc ttcatcagtg aatctaaact gcaactttag acttattagc taaagccaat
    21001 attagaccta aagctataga tgcaaatgca gaagcaatta ctctatactc agcagggaat
    21061 aagaatacaa ttgtatgaca tggtatccag aaaaatggta tagtttttaa aacactaaaa
    21121 ccaaggtaag cattccagtc cacaatattt accacatctt taagttttac cttaatcccc
    21181 tttccaacct catatttttt atctataaaa cagtccataa ccttatggaa taaaaataca
    21241 gttggtgaca ttagtatatt catagttgca ctcttgaata gagcatttgc aaaagctatt
    21301 ccctcaaatg gaagcatacc atccacttgt gcttgaccta cacctgatga gtagattgac
    21361 attccatatg tggtaactac acctattatt ccccaaacta ttgctctata aaatactcct
    21421 actggtattt tccaatcctt atacaaaatt ctagcaccaa gtaaatctcc cattgtagct
    21481 aatacagcaa actttaaaaa acctccaata tatggatgtg cacctgttgc ttccataaat
    21541 acttctctac tacttggtat tgctaagaca gcaataatac aaataagaac tccactccat
    21601 ataaaatcgc cttttttcat atttataaat actatttaat atataaatag ttctcctttc
    21661 tattaaagat ttatttaatt tacaatctat ctacaattat ttattaatca cttattatta
    21721 gatattcatt ttccatattt aaatttattc tatcccttta tctctttatt ttcttactac
    21781 tctatccacc attatctata tatttatttt acgattactc tttcacccta accctttaat
    21841 tatctatttt tttctgatat tcttatctac catcatccat atatttattt tacgattact
    21901 ctttcaccct aaccctttaa ttatctattt tttctgatat tcttatctac cattatctat
    21961 atatttattt tacgattact ctttcaccct aaccctttaa ctatctattt tttcttacta
    22021 ttcttatcca catcatccat atatttattt tacgattact ctttcactct aaccctttaa
    22081 ctatctattt tttctgatat tcttatccac catcatccat atatttattt tacgattact
    22141 cttttaccct aaccctttaa ctatctattt ttttctggta ttcttatcta ctattatctg
    22201 tatatttatt ttatgattac tcttttaacc taccccctat actttatagt aatatgtttt
    22261 taaccttttt cattattccc aatatgcttt ataactacaa atactctccc cccttaaaaa
    22321 taataataaa attaatcaat aaatttataa tcatattatt actctatgta ttaagcaatt
    22381 actatgccaa taaactttga tttttttaaa caaatcttaa aaatcctaaa atgctaattt
    22441 tctttaactt ttaatgaatc taatagataa catttaattt tcgttatatt tttcagaata
    22501 tttctattat tatttttaag aaaaaaaaat aatgttaaca ttttttaaca tacattaaaa
    22561 aatgttaaca ttataaattg ggtttaatac ttttactttg actcgattta cttttattct
    22621 acttcttact ttatttttaa cttagacttt atttttttat tacttatttg agtcttattt
    22681 attttattct cactccatat gttcaattac atttgtctaa attctttgat acttttatta
    22741 cataatttta acaagcttaa gtgttatttg ggtagtgcag ttgataatta tttaaataat
    22801 catcaactgc atgtttatat cattacagta gcttaatgta tttaatcata cttacttagg
    22861 attttgaaat atcttagaga ttatatttaa cattagtcaa ttgctgcata attgtaagca
    22921 attgaaattt gagacccatc ttcattttct atagtgatag gcttcataat ttttaatcct
    22981 tcagttttta tcttttctaa ttcttgttcc attttcttaa ttgtttcctc aaggtctttt
    23041 tgacttacat ctttgactgc attagggtct ttagcatagt cttttaattc ttgaatatgt
    23101 ttagcatact cttcataagt ataatgctca acaccatact tttccaattc agtagggtca
    23161 gttattcctg attttattag agattcaaat ttaggtaaag caatattaga tgaatcattt
    23221 ccatttgcaa atactgctcc agttcctcct agtaccattg cagataccat cattgcactt
    23281 ataagggatt tttttatttt cataaccaat cttcctttct tagagattta tttgggtagt
    23341 gtagtttaaa atgactattg caatagttat caaccacatt cttagactat cacagtagtc
    23401 atgtttatgt caagcctttt taaaaaactt ttcaataaaa aatactatct caaaacaaaa
    23461 ttaattattt tgagatagta ttttaattct aaatctttta tattactaag atttatggtt
    23521 actctatcca tgttctatat tgttctttat taactgctaa caaattccct tttcttgata
    23581 tgacaaagcg attatcatta tagccacttt gctcaattga aacttctcct gaatcagtat
    23641 aaagtacatc cttactatta ctagatttat ttacatacgt ttcttcacta ttctcagaat
    23701 tagttacttt gctcatagat gattcttcat gctctctaaa ttcaattaca gatacttttg
    23761 ccgtactaaa atgagtctct ccatcacttt tagctttact aacaagaata tcagattcta
    23821 ctttgggaga atataaatca gttgatttac tacttattga aaatccaatt ttatcacttt
    23881 gttcattttt tgttaaacca aaagattcaa cctcattaat ctctttattt tctgttgcaa
    23941 aaacaagacc tgtcccacaa actaatacag ttgaaactaa aaaagtaaca taagttgatt
    24001 ttttaaattt cataatatta actattctct tttctatttt tgtctttcca aatccactac
    24061 atattgtcat tgaaaatgga ttattctcct ctgctagact tattagtgtg gttgcataat
    24121 cagccctata ttcttcacca agtatactca taaccttttc atcacaacta aattctatgt
    24181 ctttatttag taaaaaaaac atcacccaaa ctaagggatt aaaccaatgg acaacaagag
    24241 ctacaagcat aaataacttt aacagattat caaagctatt aatatgaacc atttcatgag
    24301 ttaaaacaaa gtctaaagtt tgattattag cataattcat ttgctttggc aaaataattc
    24361 gtggttttaa aataccagat gtaagtggtg tcctagtaaa ttcagatatg caaacaataa
    24421 atcttctaaa acggctattc ttacacatcc attttttaat gaactcatga ttttttatat
    24481 gaattgaagt tttaaaaaga ttaattattt taaaatatct aaagcataaa agtatgctaa
    24541 acataatagt aactataaaa gacaaaatgc ccaacatttc caacatcata atattttcaa
    24601 tattagacat tatttcaaat tcgtcagaat tgtttttacc tgtagagtca ctagctttct
    24661 taagagtata attttctgtt gatgtattac ccacttccac atcaaaagtc gattcaatta
    24721 tactataaat actaaaacca gaatttaaac ttactggtat tagaagttta aataaaacaa
    24781 tcaaccacaa taatacaaat acctttttag gtaatttttt aaataaacat gctcttataa
    24841 aaataactat taaaagtaat actccagaag taaaactcat ttctaacaat ttaatgaaca
    24901 taaatcttac ctcactttaa tttttcaaca atttgtttaa gttttgtaat ctcatcactt
    24961 gaaagatttt ctttattaat aaaagctgaa agaaattttt ctgaagaccc tccaaacatt
    25021 ttattaatta aagtttctgt ttcctgtttt tgtacttcct ctttgcttat taaagcttta
    25081 caaacaaaat tcggctcaat tctctctatt gcaccttttg acactaattt ttttataacg
    25141 gtatacgtag tgtttctatt ccatcctgtt tcttctttta aaatttttac taactctcca
    25201 gccttgcttt ctcctctctc ccatagaact tccattactt taagttctga atcaaataat
    25261 tttatctcca tataaaatcg ctcctcaaat atagttatca caatagtcat tactataata
    25321 aaaaactatc atagtagtct atactatgtc aatcatttat tttttaataa taaaaactgt
    25381 ctttttaaaa aatt
//
LOCUS       DAEMIK010000046        25183 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEMIK010000046 DAEMIK010000000
VERSION     DAEMIK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.046"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(117..896)
                     /locus_tag="KPK87_001612"
     CDS             complement(117..896)
                     /locus_tag="KPK87_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2537768.1"
                     /translation="MKKIIWGICICLLSVVGVYTYSHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(951..1433)
                     /locus_tag="KPK87_001613"
     CDS             complement(951..1433)
                     /locus_tag="KPK87_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG2537769.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(1699..3120)
                     /locus_tag="KPK87_001614"
     CDS             complement(1699..3120)
                     /locus_tag="KPK87_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG2537770.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILALLFSKKAKHIKEQNNHKLAS"
     gene            complement(3266..4036)
                     /locus_tag="KPK87_001615"
                     /pseudo
     CDS             complement(3266..4036)
                     /locus_tag="KPK87_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(4041..5243)
                     /locus_tag="KPK87_001616"
     CDS             complement(4041..5243)
                     /locus_tag="KPK87_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2537771.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            complement(5831..6832)
                     /gene="trpS"
                     /locus_tag="KPK87_001617"
     CDS             complement(5831..6832)
                     /gene="trpS"
                     /locus_tag="KPK87_001617"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG2537772.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(6855..7553)
                     /locus_tag="KPK87_001618"
     CDS             complement(6855..7553)
                     /locus_tag="KPK87_001618"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG2537773.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(7596..7836)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7927..9330)
                     /locus_tag="KPK87_001619"
     CDS             complement(7927..9330)
                     /locus_tag="KPK87_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2537774.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     gene            9826..10902
                     /locus_tag="KPK87_001620"
     CDS             9826..10902
                     /locus_tag="KPK87_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2537775.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(11005..11847)
                     /locus_tag="KPK87_001621"
     CDS             complement(11005..11847)
                     /locus_tag="KPK87_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2537776.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            12031..12603
                     /locus_tag="KPK87_001622"
     CDS             12031..12603
                     /locus_tag="KPK87_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2537777.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(12766..13038)
                     /locus_tag="KPK87_001623"
     CDS             complement(12766..13038)
                     /locus_tag="KPK87_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537778.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(13165..14037)
                     /locus_tag="KPK87_001624"
     CDS             complement(13165..14037)
                     /locus_tag="KPK87_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2537779.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(14333..17440)
                     /locus_tag="KPK87_001625"
     CDS             complement(14333..17440)
                     /locus_tag="KPK87_001625"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG2537780.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(17533..17765)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(17940..18467)
                     /locus_tag="KPK87_001626"
     CDS             complement(17940..18467)
                     /locus_tag="KPK87_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG2537781.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(18502..19287)
                     /locus_tag="KPK87_001627"
     CDS             complement(18502..19287)
                     /locus_tag="KPK87_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG2537782.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(19297..19554)
                     /locus_tag="KPK87_001628"
     CDS             complement(19297..19554)
                     /locus_tag="KPK87_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG2537783.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(19573..20031)
                     /locus_tag="KPK87_001629"
     CDS             complement(19573..20031)
                     /locus_tag="KPK87_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG2537784.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(20071..20775)
                     /locus_tag="KPK87_001630"
     CDS             complement(20071..20775)
                     /locus_tag="KPK87_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG2537785.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(20785..21420)
                     /locus_tag="KPK87_001631"
     CDS             complement(20785..21420)
                     /locus_tag="KPK87_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537786.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            21801..22841
                     /locus_tag="KPK87_001632"
     CDS             21801..22841
                     /locus_tag="KPK87_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2537787.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(23304..24659)
                     /locus_tag="KPK87_001633"
     CDS             complement(23304..24659)
                     /locus_tag="KPK87_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG2537788.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(24685..24954)
                     /locus_tag="KPK87_001634"
     CDS             complement(24685..24954)
                     /locus_tag="KPK87_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2537789.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         8151 a         4392 c         2790 g         9850 t
ORIGIN      
        1 ataaaaaatt taaaaatacc atactaatca atactttttg tgtattttat agattaaaga
       61 ttaatgatgt ctgtaattta aacttacaag caccactaat cttcaaacaa atatttttac
      121 ttttgcactt gtttattttt atctaaatgt tgtataagtt cagatactgt tacgaattta
      181 tatcctctct cttttaattt aggaattact acttctaaag cttgtatagt ttgtgattta
      241 tcatttcttt tagtgttata atcatgtaaa agtattatat taccattttg gacctttgta
      301 aggatagtat ttataattgc atctactcca gggttagacc aatctctagg gtctaagttt
      361 gaccaaagaa ttatattcat gcctttagaa actataatat cacagacact tttactcatg
      421 gctctatatg gaggtctaaa tatctttggt tctactccta ttacactttt tataatattt
      481 tgcgtatctg ttatttcttt ttcaacacta ctataactat tttttgcaac atttatatgt
      541 gtaaatgtat gattccctat ttcatgcccc tcatcatatt gtctttttaa taattctttg
      601 ttatattcta ctttttctcc aactacaaaa aaagttgctt ttacatcatt tttctttaat
      661 atatcaagta cttgtggtgt aaaatcctca tctggaccat catcaaaagt caatgctaaa
      721 attttttcat cctcatttcc tttttttatt attacatctt cataatcgtc ttttatattg
      781 tcatttaaac tatatgcttc ttttgaacta tcttgattat gtgtattatg actatatgta
      841 tatacaccta ctacagataa taaacatata catatacccc atattatttt cttcatgtca
      901 ttacctcttt tctaattcat tctagcaata tattttataa tgtcagtttt ttaatctctc
      961 aatattccat ttttatttaa atcagttaaa tttgttaggt cttctgaata aaagttagaa
     1021 tcttctacat cagggttttg cattgcatca cctaataaat tattttcttt ctttgcacaa
     1081 tcattacata aacttgtatc tggcaatata tccaatcttt ctaaatctat ttgattatga
     1141 cataaatcac aaatcccata tgttccattc tctattttag ataatgctaa gtctatttga
     1201 tacaatcttc cctcttcatg atttattaga ctgttttgca tattttgcat atataaatct
     1261 gttcccatat ctcctatatg attatcataa ctagataatt ctcctgatga atacttttca
     1321 cttgtatgtt tagttgtatc tccaaataaa gtattatctt tcatatcctc tactaaatta
     1381 gttaaatttt ctctttcttt caataattta tctttatatt ttttagtatc catttttaac
     1441 acctctaatt ttaatattta tatcataaaa atagtatgtg gaaaagtgaa ttaaacatac
     1501 aataaaaata agatttgatt ctaaaaatgg aatgtctcaa aatgaaaatt ttagttcatt
     1561 taaccctctt ttttatctga aatcaaatat atttttatcc tttcaacaat aaaaaagaga
     1621 cttatctcta ttttgagata gtctcttctt tgaacataat ttaaatataa ttttttaatt
     1681 tttatagtta ttattttact aacttgccaa tttatgatta ttttgttctt taatatgttt
     1741 tgctttttta gagaataaaa gagcaagaat tattgctaca ataacaccta ttccattaga
     1801 tatattaact ggtagtctaa atccttcttt tgcttgcatt atataactaa atgttactac
     1861 agtcataaaa agtgctggta atgtagttat ataatggttt ttatttgctt tgactaaata
     1921 tgcagaacca gtccacaaca ttatcattgc aagtgtttga tttgcccaag agaaatatct
     1981 ccaaattata ttaaaatcta taaatgtaag agcaacacca actgcgaata atggtaaagc
     2041 tattaaaaat ctatttttga ctgcatcttg attataattc attgcatctg ctattgtcag
     2101 cctagcactt ctaaatgctg tatctccaga agttatagga catgccacaa ctccaagtat
     2161 agctaaagct cctccaatag gacccattaa tccaacagat actgtattta ctactgttgc
     2221 tgctccacca tcattaaata tcacattaag ttgaggtatg ccatggaaat atgacatggc
     2281 tgctgctgcc caaactaaag ctattatacc ttctgcaacc attgacccat aaaatactgg
     2341 tcttgcctca ctctctttct caacacatct cgccataagt ggtgattgag ttgcatgaaa
     2401 acctgatatt gctccacatg ctattgttat aaataagtat gggaataaag gtgttccatt
     2461 tggattcata ttttgtaaat ttatatttgg tatgttatac ccctttatta ttaatcctcc
     2521 agctacacct actgccataa ttaataatgc tgctccaaat aaaggatata ttttaccaat
     2581 caatttatct attggtaaaa cagtagccat aatataataa agtattatca ctccaaccca
     2641 tatttgtgcg tttacacctg tcaatgtctt taataatcct gctggacctg ttacaaatac
     2701 aactccaacc aatactaaaa gaataacact aaatattctc attgctattt ttgcaccatt
     2761 tcctagataa aggcctacca actctgatgc agatgtacct ttgtttttca ttgatagata
     2821 tcctgaagca aaatcgtgta ctccaccagc aaagattgtt ccaaacacaa tccataaaaa
     2881 tgctgatggt ccaaataatg ccccttgtat agctccaaat atcggaccta accctgctat
     2941 atttagaaac tgaattaaaa agattctctt ccatttcatt ggcacataat ctactccatc
     3001 cctacaagct atagctggag tttgattttt gtcattggtt ccaaaaacct tttctacaaa
     3061 agtaccatag acaaaataac ctactataag tactactatt gaacctaaaa agcttaccat
     3121 aaattcatcc ccttagtttt tatataattg ctttataaat ttttatgagt ttaagaaaac
     3181 tatttccagt tcaaatagta atactttttt atgctaatat aaatattaat tacattaagt
     3241 gcatatttaa gtacatgaaa tgactttata tatgcattaa cagcctaaat tcttttactt
     3301 tgcttctact tacagttact tcataatcac tattttttaa ttttaatatg tatgtattat
     3361 taaaccatgg tataatttct tttactttgg ttaagtttac gatataagac ctatgtgttc
     3421 taaaaaatgt tttatcattt ataagatttt cgacttctta tatgccttct tttaatataa
     3481 acttttcttt ctcagtatat ataaatgtct gcctttcatt agcttcacag taatatatat
     3541 catcaatatc tattacaact aatttatcac ctttccaaag gctaatttta tgtgttattt
     3601 cttctatctc ctgatttact gcttccttct tatatttgta taaattacta tttacattag
     3661 atagttcaat atcactcatc tcactttttt ctaatttatt cagcatagaa ataattctct
     3721 cttcagaata tggtttcaat atataatcaa atacatccaa agaaaaagca tctactgcat
     3781 aactttcata tgctgttata aaaacaattt taggtcttga tttaaactga tttaatgttt
     3841 ttgcaagtag cattccatca agatgaggta tatttatatc taaaaaaatt acatctattt
     3901 tattttcctg aataaagtct aacacctcta taccattggt aaattcactt actacctcta
     3961 ttccactttt attttcaata aaatatctca attcctttct tgctggaaat tcatcttcca
     4021 ctattatagc tctcatactt ctactccttt acataaaata ctatcaaagt cccattatct
     4081 attcttctta tattaagccc tcttccatac atcagtttaa gccttaaatg tacattataa
     4141 agcccaatct tattttctgg catattatct tgatatacat tatttataat ttcctcactt
     4201 atacctatac cattatcctc tattgatatt ctcacagtat tttctaatga atacggtaac
     4261 ttttttatgg atattttcac tacaccagct ctaccacttt ctaaaatacc atgtattatt
     4321 gcattttcca ctaatggttg tataatcaaa cttggaatct ttatatttac atcttctata
     4381 tcataagata cagttagtaa ttctccaaac ctagcttttt ctatctctac aaaagattta
     4441 acttgttcaa tctctttatt tatatcaatt aagttatcac tatattcaag attatatctt
     4501 agataagttg ataaatttat tattaactct ctagccttat ctggactaaa tcttataaat
     4561 gaagctattg tgtttaggga attaaacaaa aaatgtggat taatttgtct ttgaagtgct
     4621 tttatttctg ctttatttct cgcttctata tcttttttat ctttctcaat cccctctact
     4681 atagatataa caaatcctat tccaatctgc ccaactatta aaggtaaata tattccaccg
     4741 attatttgaa ttgctaaaga atgtggatat gatattaagt atataagtaa tatagtaaaa
     4801 ctttcagaaa tcatgcctac caatattcca tacattactc tatgttgttt tggtatcctc
     4861 ttatatagaa atcctgataa tacacctgct agtatacttg ataaaagaca tggaacagat
     4921 gttatccccc caatatccat aaaatatcta tgaatccctg aaaaaactcc tgctgtaata
     4981 cctaccattg gaccaaataa aatgccacct gatactatag atattattct agtgtttact
     5041 attgacccca tataatttat accactataa gttccaaata tggctaaaaa agtaaaaact
     5101 aatgctatac agaccaagtc attaaaacta tattcttcct tttgaaatat ctgcttaaat
     5161 atttttaact ttgttattag aagaaacaat actacaataa ggcttgtctt ttctattaat
     5221 gcgattaaaa ttgatgtttt cataatcccc ctttaaaaaa taaaacatta cttaatttaa
     5281 gtttattatt cttataataa attagataca taaaaataaa ttccataatt attgtatcat
     5341 atcacctata tataaacata aattcattta attatataaa tttaaaaaat atttttttga
     5401 tatattattg acaattttca ttataagagt taaaattaac tcataaatta catattgggt
     5461 ataaaataca atgtatgtta gaagttgttt cagaaggcta tggttttctg atttttttat
     5521 gctcagtagt gatatgcaaa aaagacaaag ttgacatcag ttaactttta cctttattct
     5581 actttcccca aagtaagaat aatctaaccc ctttgagatg agcaaaagct catctttttt
     5641 tattatcaaa agatgctgtc tcaaaacaga gataatcctc tttttcattg ttgaaatgat
     5701 taaaatatat tttatttcat acaaaaaaga gtgcctcatg aacaaaagtt cactttgaga
     5761 ctctctcttt catatcatat acctttttaa taactttaaa atttatttat aaagtaaaga
     5821 tagctaataa ttagtatctt ctctctataa atccaacttt tttataaact tttctaagag
     5881 tttttctagc ttgtttttcg gctttttctg ctcccatact atatattttt tctaaataat
     5941 ctttattatt aagaaggtca acatattttt ctcttatagg tcttaaagct tctgatacta
     6001 cttcagccac atcttcttta aatatgccat aaccctttcc ttcatacatg tttacaatct
     6061 cttctacact ctttttactt aaattagaat atatatcaat tagattttta atacctggtt
     6121 gttcatcatt gtattttaca acacctaaag aatcagtaac acttcttttt atctttctct
     6181 ttatagaatc tgaatcatct gctaatagta taaatgcatt ttcatttgaa tcggatttac
     6241 tcattttttt agttggctct tgcaaactca tcactcttgc tccacctttt gggtagtatc
     6301 cttctggaac tacaaaagta ggtgaaaatc tattattaaa cctatttgct aaatcacgag
     6361 ctaattctaa atgttgtttt tggtcatctc caactggaac taggtcagtt tgatacaaca
     6421 atatgtcagc agccataaga actggatatg tgaataaacc tgcatttaga tttgcttcac
     6481 tcttttgtga tttatctttg aattgagtca ttctacttaa ttcacccata taagtcatag
     6541 tattcaatac ccacataagt tcaacatgct ctttgacatg tgattgtata aatatagtat
     6601 ttttttctgg gtctaatcca caagctatat attgagcaag taattctata gtattggctc
     6661 ttaaaacttt agggtcttga ggtactgtta ttgcatgcaa atcaactata ctataataac
     6721 aatcataatt gtcttgaagt tcagtccagt tttttatagc tcctaaataa tttcctaaag
     6781 tcattttgcc tgatggctgt gcaccactta atataacttt tttttcactc atttttttgt
     6841 acctccttat atatttatat tgattttaac atatttaaaa ctatgtcttt agagttattg
     6901 gcagcatcat gtacaaatac atcataagta acatcagcac ttccatctgc cttatcagac
     6961 attgctctta ttattacaaa tggagtatta tttaaatagc atacatgagc tatagctcca
     7021 ccttccattt ctccacaata tccaccaaaa tcatttacta attcttcttt taattcttta
     7081 gaatttataa atttatctcc tgtaactact cttcctttta atactttatg agtctttact
     7141 tcttccacag aagatttgta agcagcatct ataagtcttt catctgcttt aaatacagaa
     7201 gtcttcattc ttggtataac tcccttaggg tctccaaatg ctgtagtgtc tacatcatac
     7261 tgtatagcat cagttgatat tactatatca ttaacatcaa gtttttcgtt taaagctcct
     7321 gcaacacctg tattaacaat agcatcaacc ttaaattcgc ttattagtat ctgggcacaa
     7381 agagcagagt taaccttacc tatgccacac tttactaaca ctacattctt accttccaaa
     7441 attcccttat aaaattctaa gctagctttt tttatagtct ctctaatatc cattaaatca
     7501 actaaaatac taacttcttc atccattgca cctataattc ctattacgtt catttttcct
     7561 cctaaatttt ctatattaaa aaatccgtcc taaatcaata ggacggatat attttccgtg
     7621 gtaccaccta aatttatata cactcaaaat tgaatatata tatcttaata tatgctataa
     7681 cgtgcataca cgatatcaaa tactctgtaa tcttaaatta catttcattt tactcctcaa
     7741 agacccattc aataaatact ttttattaag ttctcagcat ccttaattct ctgtgcaaaa
     7801 atatatttat ttactatttc ttatctcagg atttaattta ttaatttctt tattttataa
     7861 tattccttac actataaatt gtcaactata tattataata tccaaaagaa atttttaaaa
     7921 tacattttat ttagctgttt tcttttcata tctattccat aaccatagac cagataatat
     7981 aactagaaga cctccaccaa tcatctctat ctcatatata acattttcag caaatgtagg
     8041 cataactggt gctgcagata ataatatacc cactacagtt acaactaaaa ccatttttgc
     8101 taaacctagg caagtacttg ttgattttgc catagtataa ggtctaattt catctggtct
     8161 ctcttttctt agtttaatat aagaagcata taacaataca tatgggaata atgcagttaa
     8221 tgctgtcata gttacaagaa cattgtatat agcatcaaca gaaggcatta atgtagtcac
     8281 caatagtatt atagtaacta ttattgcttg aagtataact gctttagaag gtatcttgtg
     8341 ttcatttacc tctactaaac tatctggaaa tattcctttt ttaacactac caaataacat
     8401 ttttattggt gatgctatgt aaagaactat tgctcctaat actgaaaaag ctataccaaa
     8461 agctattaat tgaaccaacc aagaaccaat tcctaaagac gcacaaacag cagaaagtgc
     8521 atctaataca cctttagaag cagtaatttg gtctggtggt attatcattg ttattgcaat
     8581 tgaacctaat acatataatg aagctactat tccagctgat attaatattg ccttagggaa
     8641 gttcttttca ggcttatcta tttctgttat gaagttagca gcagtctctg ctcctgctaa
     8701 accaaacatt atagctgata tagcaacaaa tgcatccata ttaagttttg gagtcattga
     8761 agctattgtg tatgtagaag ctatatcatg ttttttcaac acaaatactg ctacaaatga
     8821 aaatattata agtatagcac ttggtattgt tgaacctaat gcacctgtat tagtaaatat
     8881 ttttgtaaaa cttattcctt tagttgctat aagagataat gcccagaata ttattaaaga
     8941 taatataagt acaaacatct tattatcagc aagggctgga tttcctattg tataagaaac
     9001 atttactgct aagaaagtta aaaatgaaga ataccaaaat attttagctg tccagtttaa
     9061 ccaagaaact aaaaatccat acttttctcc aaaggcttcc ttaacccaat cgtaaagacc
     9121 tccatcacct ttttgatatg tagaagctaa ttcagcacaa attaaagctc ctggtacaaa
     9181 gtatataaat gagaataata accacatagg tattgcacct aatcctaaac caaagcttga
     9241 tgctgtagat ttagctatcc atcttatacc atataaagct gatacattca taaataccaa
     9301 actccatagg cctatttttt tcttttccat cctaatttaa tttcccctct ctattaatta
     9361 ttttaaattt aatttcaaaa ttaataaaat agattcatta ttattaaact gatatttttc
     9421 aaaatatttg catattcaaa aacagtttta ataataaacc ttaaatactt tgaaatcaaa
     9481 tcttattaaa aatgataaat ttaaaagttt taccacttta attctaatga tactaaattt
     9541 ttaaaattgt ttcaatatta ttttattata aaattttatt tattattttt cattagtata
     9601 taaaaagata aaatacataa ctttatagac ttaatttatt acttttataa aattttcaca
     9661 ataaaaagtc attttacttc cgtatttaat gcaaaagtag agattaaact taatataaaa
     9721 aatatataac aaattttttt atatttctag aagaaaagta tactcttttt tgtttaataa
     9781 tgctaaaatt gatttgtatt gaattttgat taggaggaaa ttattatgaa agctcaaaga
     9841 ttaaatgctg tacttgaaca aatgaaaaaa gatgatattt ctcaaatgtt agtaagtgac
     9901 cctacttcta tattttatct tacaggagta ctaatccatc caggcgaaag actattagca
     9961 ttatacttaa atttaaatgg taataataaa ttatttataa atgaattatt cccagtttca
    10021 gaagatttag gtgttgaaat ggtttggttt aatgatactc aaaatccagt agaaataata
    10081 actgaacata tagacaaaaa tgctactatg ggtgttgata aaaactggcc agctagattc
    10141 ttattaaact taatagaatt aggtggagga agtaagtttg taaattcatc ttatataata
    10201 gatactttaa gaatgtgtaa agatgaagaa gaaaaagagt taatgagaat agcttcaaaa
    10261 ttaaatgaca aggctatgga acaacttaaa gctactgttt ctggtgaact tacagaaaaa
    10321 caacttgttg gtaaattatc taaaatatat gaagacctag gtactgatgg attttcattt
    10381 gaccctatta taggttttgg acctaatgga gctaatccac atggtgaacc tggaaatgca
    10441 cttgtgaaac caggagatgc aataatatta gatataggtt gtataaaaga taactattgt
    10501 gctgatatga caagaactgt attctataaa gaaatacctg aaaaaggtag agaaatattc
    10561 gaaatagttc ttgaagctaa taagagagct gaagctattg taaaaccagg tgttagattc
    10621 tgtgatatag atgctgctgc tcgtgactac ataactgaaa agggatatgg acagtacttc
    10681 actcatagaa ctggtcactc tataggactt gaagttcatg ataaaggaga tgtatcttct
    10741 ataaatacag atactgtaca accaggaatg atattctcta tagagccagg tatatattta
    10801 ccaggtgagt tcggagttag aatagaagat ttagtacttg ttactgaaga tggttgtgag
    10861 atattaaaca aacatgacaa agaaatatgt gtagttggtt aattctaaaa tcaatttgat
    10921 aaaattaaaa agccatataa aaatgtttta aacattttta tatggcttta atttctcaat
    10981 ctatgattta tttcaatcca ttatttatat tgcagttatt aagattgcac cctttgaagt
    11041 atatgtaccc atcactgaac caacttcaca gattacaaca tctttgtatt ttacatcctt
    11101 ttccataact tcccttacct tttgagcctt ttctgggcaa ttagcatgtc ctataaataa
    11161 aaccttttct tctttatctt taatttttga ctctactaaa gtaatcagct ttttcaaact
    11221 gtttccttcg cctcttgcct tatctatagg agttacaacg ccatctgcaa cttgtattat
    11281 cgccttaaaa ttcaaggtat ttataatttt tcctgctatt ggatttattc taccaccttt
    11341 aatagcattt tctaaagttt caagtgttcc aaaaaatgga ggattatctt tatatttatt
    11401 tattattgaa acaatctctt caatttcttt tccttctttt attaatattg ctgcttttaa
    11461 cactaattgt cctattccaa ttgacccact aagtgaatct ataacctcaa ctttcttgtt
    11521 atttccttcc ttttcaaaca tatcttttgc taaaagtgca ctattataag tcccagataa
    11581 tcctgaactt atattaatca ctaatacact ttcttcctga caatggtatg cttctaaaaa
    11641 tttatcagga gaaggacatg atgtttttgg tagtacttta ctctcactca tttttctata
    11701 gaaagtttca ttatctattt cctttccaga tatataactt tcttctccaa aagatacatt
    11761 taaaccaaca acttctatgt tgtatttttc tattatatca tcaggtaagt cacatgcact
    11821 gtccactatc aattttattt tatccatatt aacctcacta taagatatta ttttatttat
    11881 tcagtcggtc gtacgaccga tttaattatt attttatata ttgaatgaaa aaaagtcaat
    11941 agatatagat tctcgcttta ttgacaataa atatccataa gcttataatg ttattaattt
    12001 gttataaata tacatggaaa ggtaggtttt atgaaaacaa ataatatgaa acaggctatt
    12061 ttatcatctg caaccaattt aatagccaaa aatggagttc aaaatactag ccttgctgat
    12121 attgcaaaag atgtaaatat aagtaaaggt actttgtatt atcactacgc atctaaagat
    12181 gatattatat atgatattgc ggatactcat ttagaagtta taactagtgc tatactaaac
    12241 tgtgtaaaaa atgttaaatc aaaaaattct caaatagaaa tggttaactt aatcttagag
    12301 aaaatatcta ctatagaaag tcgtggtagg gtacatatgt atcttatttg tgaggcaatt
    12361 acttcaaata atgatttaaa ggaaagaata agattaaaat atattgaatg gagaactact
    12421 cttaaatctg aaattgttga atcactagaa aaatataata gtagcgaaaa tgaacaggat
    12481 gctgaatcat tttctttttt actcatgtca atagttgatg gtttagttgt ccaaagttta
    12541 ttaaaaacag aaaaaatacc ttatgaaaat atagcttctt ttttagttaa tcattggatt
    12601 taaacttaat acttagataa ttaataaaag tgaactattt aagttatcta ctttaacagt
    12661 tcactttctt atttattctc aataattttt acttaaaaca ttcaattttt atttaaacct
    12721 tcatggaatc acaaaataaa aatctcttat aaatttaaaa tgtttttatt tatgttcatg
    12781 tgtatgccca tctgaatgtt catgaccatg tgtatgtgta tgctcatggc tatgctcatg
    12841 agtacctgaa aaaccatcat catgattatg gtcgtgacct acaccattta catgctcatg
    12901 ttcatgccca tgactgtgag aatgttgatg tgaatgttca tggttatctc ctccacagca
    12961 tccttctcct ccagtcccac aacacccact tgtatgtgga ttatgatgat ttcttactac
    13021 aaatctctct cttaacataa ttcttatacc tcctagtata ttattgagta cttcatgtac
    13081 cccattaact acagtataca tctaactagg tataatctca atacatatgg cattttttaa
    13141 catatataat taacatatat aatcttaaag aaatttatcc ttaaaacctc caccttttac
    13201 ttcttcaacg ttattgacag aaataaaagc ttcactatcc atatcataaa ttaaatcttt
    13261 aaatttacct atttcatttg cagatattat acaataaatc atctttttct tattttgtgt
    13321 atatgcacct tctgcctcca aaaaagtgac tcctctaccc attttattca ttatcacttt
    13381 ggatatttca tcatatttat ttgatattaa aagtatagat ttttttctat caaagcaatc
    13441 ttttcctata tccatagtaa aagaaattat atacattgta attaaagtgt acatcaccaa
    13501 tttagctcca aataaaaaag ctcctgcaca tactacaaaa aaattaataa ataaaaatgt
    13561 atttttcata ggtatatcat actttctttt cattatggct gctattatat caagtcctcc
    13621 agcagtagct cttgctttaa aagttaaccc catacctata ccagttaatg caccaccata
    13681 aatacactgt agcatagtat catctattgg gaaatattta gcaatatctt gtgttagccc
    13741 cagtgctaga gaaaatagta acatattgac aaaacttatt agcaaaaatt tattatcaaa
    13801 atactttcta gctataataa atattggtat gttcatcaag aaacttgata ttccctgatt
    13861 tataccaaat aatttgtata atataacaca aattccagct actcctccac ttaataactt
    13921 ggctggtgct agatataaat ttattcctaa ggttgataga atcataccca atataataag
    13981 acaatattgt aaaaatatat tattatcaat acttagcttt ttactttcac catacatcaa
    14041 aacgcacccc tttgcataat aaataaacca ccctgcataa cacacgataa atttacaaat
    14101 aaatttatca gaagtagttt cctaaaaata ttattcatcc tcacttaaac gtttattcat
    14161 taatattcta tcacatgaca taaataattt atatgattta tatagattta tttattgtaa
    14221 attttggaat attaaaacat tttattgaat agataattat attacataaa aaatccctat
    14281 agaaatctat aaactaattt ctatagggat tttggttaac taactattct ttttattttt
    14341 ttataacttt tatacctgtc atatggtcat ttaagttttg ttcctctaaa gatttatcat
    14401 ctactctgtt tatttctaaa gctaaagttt cagacttgat atattcatta aaatggtcaa
    14461 cagccttagc aatttcatca tcaccacagt attctattac tatattatct aatacttcaa
    14521 agttattaga ttttcttaat tgctgtactt tagatataaa ttctctagca taaccttcat
    14581 taatcaattc ttcagttagc ttagtatcta gtataacaaa aagattgttt tcaacagata
    14641 cattaaatcc ttccttagca gttataccta ttaatactaa atccttatta aattcaaaag
    14701 cttcaccatc taaatcaact gttaaagtct cacctttttc aagtttatta gcagtttcat
    14761 gagcatttaa tttacttaat gctccaacga ataaattcat cttagaacct aataatggcc
    14821 ctgcttcctt aaagtttggt tttaaactaa agttcatata ctcatctaaa tccttagcaa
    14881 atattacctc tttaacatta agctcttctt ttattaaatc aactacatca cttattgtat
    14941 cttcaaactt accatctact aacacttttt gtaatggttg acgaactttt attctttcta
    15001 cttctcttga agctctacct aaagtaacta agtttttagc taaatccatt ttttcttcta
    15061 atttagtatc tactaactct tcattacatt taggatattc agctaaatga acagatactt
    15121 ctcctgttaa gtttctatac atttcttctg acatatatgg tgcaaatgga gctatagctc
    15181 tacaaagacc atgtaacaat tcataagttg tattgtacac tgctttttta tcttctgtta
    15241 attcagttgc ccagaatctt cttcttgaac gtcttatata ccagttagat aaatcatcat
    15301 ttataaaatc ttgtatcatt cttaaagttt tatttaactc aaatatagct aaattttctt
    15361 ctacatcttt cattaaatta ttgaacttag ataatatcca tctatcaagt tctggtctat
    15421 ctttatattc tacaaaaaat tcagttggat ttatatcatc tgtgtttgca tataaagtaa
    15481 agaagttata tacattcttc atagtaccta taaatttact ttggatttct tttaatccat
    15541 ccatatcaaa tttagttgga gtccatggtg gagaaacata tagtaaatac catcttaatg
    15601 catctgctcc atattggtca aataattcca ttggatttac tgtatttcct cttgatttac
    15661 tcattttctt accatcttta tcaagtacaa ggtctggaac taaaacactc ttatatggtg
    15721 cttttcctaa tacaaatgtt gatactgcaa gtaatgagta gaaccaacct ctagtctggt
    15781 ctataccttc tgatatatag tcagctggaa acaattggtc aaagttttcc ttattttcaa
    15841 atggatagtg gtgttgagca aatggcatag ccccactatc aaaccaacag tctataacct
    15901 ctgttactct tgtcattggt tttccacatt tttcacattt taagtgtata tcatctacat
    15961 atggtctatg aagttcaact gtttctggat ttacatcttc tatagctttt tcagctaatt
    16021 cagctcttga accaactgaa tctgtatgac cacattcaca tctccatata ggaagtggag
    16081 ttccccaata tctagttctt gatattgccc agtcatttag attttctaac cagtttccaa
    16141 atctcttctc tcctacaaaa cttgggaacc attttacacc attattattt tctattaatt
    16201 tatctttaag tttagtcatc tctatatacc agcttggttt tgcatagtat actaatggag
    16261 tttgacatct ccagcagtgt ggataattat gaactacttt ttctttgcta tataatttat
    16321 tttcagaagc tagccatttt attatttcaa catctagccc atcttccatt acaaatctac
    16381 cttcccaagg agtagatgta aatttaccag tttcatctac tggttgaata actggtaaat
    16441 catatctttt acctgtgttg tagtcatctt caccaaatgc tggtgctgta tgaactatac
    16501 cagttccatc ttcagtagtt acataatctc ctactgtaat aaaaaatgct tttttgtcaa
    16561 cttctacaaa aggcattaat tgttcatatt ctaaaccttc taggtcttta cctttcattt
    16621 cttctaaaac ttcataatct tcacctagta ctttatcagc taaaggttta gatacataat
    16681 atacctcatc acctttttta acttttaaat agtctacatc agcatttact gttaatgaaa
    16741 catttgatgg taaagtccat ggagttgttg tccatgctaa gaagtattca tcagcatctt
    16801 ttttcttaaa tttagctata actgtattag tttttacttc tttatatcct tgtgcaacct
    16861 catgactagc aagaccagtt ccacatcttg ggcaataagg aagtatttta tgcccttcat
    16921 atatatatcc ttctttgttg aatttgttta gtatccacca tacagactct atatagttat
    16981 tatctaaagt tatatatgga ttatctaaat caacttcata agccattctc tcagtcatta
    17041 ttctccattg tttttcaaat gagaaaactg actctctaca tttttggtta aattcagcta
    17101 ttccatattc ttctatttgt tgcttactag taagtcctaa ctctttctct acttgtattt
    17161 caactggtaa tccatgtgta tcccaaccag cttttctctt tacttgataa ccagacattg
    17221 ttttatatct acaaactgaa tctttaagcg ttctagataa aacatggtgt actccaggat
    17281 ttccattagc tgttggaggt ccttcataga atacgaaact tgggtcatct ttcccttttt
    17341 ctaaagtttt ttcaagtata tttatgtctt tccaataatc aaaaacttgt gcttctgctt
    17401 gttttacaga actgtctacc aatggcttaa atttcgccat gtaaatcacc ctttcctact
    17461 aaatattata attccaaaat tttttattct ttcacataaa tttaaatact aaaaaactcg
    17521 ccccctttaa accaaaggga cgagttataa atcgcgttac caccctaatt ctatatgtaa
    17581 actaaatact agctacatat aacactctaa tagtataacg gtactaaccg gcacagctta
    17641 gtagaattta atcgttcagc ctgcagctca agagtgattt ttatctttat aaattcattg
    17701 gttctcagca tacccaattc tctgtaaaat tatctaaaga taaatgtctc tatcaacgct
    17761 tttaaaaatt tatctgtgtt tatatgaaat tctataacaa ataatttgaa tagtcaatat
    17821 atttttgaaa aatataataa tttctatttt tcttacattt ttatcctatt tttaatcttt
    17881 gttatagttt tctcaaactc acacaattat tctacataaa aaataaacaa attacaagct
    17941 tattctaaag ttgtagcagc ttcatcatta ctataattat acactgttgt tccttcaaat
    18001 gcatttacac atttttcatc tacatcataa aaaatctcat caacacttct tatttcatct
    18061 tctaacagtg atttaaattt atttctaaat atcttaaatt ctttaactaa agcatcatac
    18121 tcttttctta tctctataac acggttatta gcttgttcta taatttgtct agcttttaaa
    18181 tcagcttctt caacaattat cttagccttt ttattagccg cgctacaagt gtcttctgct
    18241 gcactttgag cagttattaa agtagctttt agtgtttctt caatattttc atacttgttt
    18301 acttggtctt gatataaccc taatttttct ttaagagctg tattttccct acatagagat
    18361 tcataatctt ccttgatgat atctaaaaat tcatcaacct cttcatctct ataccctctt
    18421 aaaccttttt taaactcttt attttctatc tcaattggag ttagcattac atttcctccc
    18481 agctataatt taaattagtc cttatacaat taatcttgct tgaactttta tttttccttt
    18541 tttagtaata tctcctattt gtgttacctt agctcttcct ttgcctctaa tagaaatcaa
    18601 atcatcgttt tttactattt tagaagttga agttatcttt tcataattga catggacttt
    18661 ttctccattt atgtattttg cactatcttg tctagatata ttgtaaatac cacttatcac
    18721 acaatctaac ctatctgaag atactgtaaa tgatacttct ttatattttg aagaactatt
    18781 aacaatattt tctctggaaa tttcctttac aattacatta tttcttgaaa ccttttctaa
    18841 gtttacaatt ataaaatcgc aaatatcact acttacaact acttgacaaa agctatcatg
    18901 tataattata tctccaattt tttctctttt tatacctaga cccagtattg accctaagta
    18961 atctttatga gatatatttt taaatttgaa attcccttct atttgtaaaa atctaagagt
    19021 atcttctata tcttcatatt ccatgtaaaa tggataaata aatacagtgc ttctttcagc
    19081 ttgttcataa cctccatcca cactgtattt tatttcattt gtagaattta atattgctac
    19141 agcatttttc acttcatatg gattaagaaa ctccgtagac ttaacatcat agttttttat
    19201 acaactatta gccttgtcta tcactttaaa cattttattt ttcaagtcta tatcctttat
    19261 gtgatttgtt agttttaatt tatccatcct atcaccttag aatataacca atactaattg
    19321 tcttaggaac atcaataata ctattgcaat taaaggagaa aaatctatag gtccatttgt
    19381 atatttatac attatagacc ttattggccc ttctattggc tctgtaagat tttccaaaat
    19441 atcaaaaatt ttactttgtc ttccatttgg aaaccaagtc atcaagcttt ttataactat
    19501 catccatgct attatatcta aaagataata aagtgcaatt cttattgttc ccatatatta
    19561 accgcctttt tactatttat tttgccaagg gaagaatgct ttactttcta attctttttt
    19621 catatttgca tctatatcca cattattagg agctagtata aatattgatt tagaaacctt
    19681 ttgtatactt ccatctaaga cataaactgc cccattcata aagttaaata tagatttgcg
    19741 aacttcagaa ttagtcaatc cttccaaatt cactataact gcccttcttt gttttagatg
    19801 gtctgctata gttgcagctt catcataaac cttaggttca actattacaa ctttcatttg
    19861 gctagttgta tgtaaattta caattttatt tgcttttgca gtactaaagc cactgttcat
    19921 ttcttcttct tgaactatat cgtccatgcc actttcatat tcatcttcaa tgtaatcttc
    19981 ttcttcatca actatccaat tcttaaattt agatattatt ccatttgaca ttattaactt
    20041 cctccttaaa atttattcaa atttgagtaa ttatttttta ttgtagtttc tttctccaaa
    20101 gattgatgtt ccaactctaa caatggtagc cccttcctct atagcaattt cataatcgtg
    20161 actcattccc attgacaaaa catccatccc tacattaggg atgtttagtt cttttatttg
    20221 ttttgataaa tctttaagac ctttaaatac acctcttatc tcatcttcat cttctataaa
    20281 aggtgccatt gtcattagac cctttatttt tatattttta tatttttctg acacttcttt
    20341 gacaaagtct acaacttctt ctctatcaag tccatgctta gactcttctt tagatatatt
    20401 cacttgtact aagcaatcta tttctctgtt tatttgttct gcacgttttt gaatttcttc
    20461 gcataatgca attctatcta aagagtgtat catacaaact ttatcgatta tgtacttaac
    20521 tttattggtt tgcaaagttc ctatcaggtg ccagtttaat ttatcaccta taatatcata
    20581 ctttctagct aattcttggg gtttattttc accaacagaa gtgattcctt ccctcattgc
    20641 ctcattaact aagtctacat caacagtctt tgtgactgcc aaaagggtaa tctctttgct
    20701 gtctctatct actttttttg cagctttatc aattttgttt cttatctcag acaaattatc
    20761 tttaatagaa cccattatta tcacctactt tatccttata tttgtgttta tactgttagg
    20821 tcttaaaatt attttgtcat atagattaac acttgtaact ccatttatgt catttttata
    20881 ataatccaca aatacagaag attcattctc ataagttatt ccttctaata tgacaaattt
    20941 tacactttgt gattgttcat caattacata tacaccacgt ttattgtctt ttgtttttat
    21001 agaagtttta ggtattttta aacattccat ctgattatat attatatcaa attcttcgac
    21061 acttgtatcc tgatttgcaa caatttggtt acttattttt aattttacca ttattttatt
    21121 attattttca tatattttgt aaacttttgc atctaatttt tgctcatgat attcaattat
    21181 tacattttga tttttttcaa agttattaga atcttcttta ctgatgtatg tagatagata
    21241 attactctgg tcattggcat ctttttctat agttcctttt acatgaatct ttgcatcaac
    21301 tttttcattt tttaacacca acgtatctac atttttgcta attatcatta ctaatatatt
    21361 aaatactaaa tatataagaa taccaagtat cactaatctg gtatatttta cctttttcaa
    21421 agactctccc cctataaaat tactttcatt ataagtactt cttctaaaaa atgtttattc
    21481 cttttttata agctaatatt ttacgttcta ataattattt tttccatatt aaatttattt
    21541 tttatcaatc ttcctataca agactgcatt atttatatat aattgtatta taaagtataa
    21601 gtttaattta cattttatac gttaagctta ctttatatgt ttttatagtt atttttattt
    21661 ttagatgcaa tataaaacca attaagattt aaaataactt ttgttatgtt gacatttatt
    21721 taggtaatca tttatgaatc aggataacgt tatcataaca aaagtttatg tcctaaaaaa
    21781 aatacaagga ggattttctt atgtcagaaa ttttattagg aacaggttta cttttattgg
    21841 ttctatgttg tttcacaatt tttagttaca aggccccata tggtatgaag gctatgggag
    21901 ctctagcaaa cgctgcatgt gctactttct tagtagaggc attcaacctt tctttacttg
    21961 gtgatgtttt aggtatggaa ttttttagaa gtgttggagc tgctaatggt agtcttggtg
    22021 gagttgctgc tgctatactt gtgcctttag ccttaggtgt atctccagtt tatgcagttt
    22081 taataggatt atcttgttta gatttttcta tattatctgg attcgttgct ggatactttg
    22141 tttctttctt aattaagttt atagagaaaa aagtgccacc tggagtggat ttagtggttg
    22201 taattcttct atgtgcacca ctatctcgtc ttatagcatc aaatgtagac ccattagtta
    22261 atagtacact actacaaatt ggacaagttt tgatagaagc taccaacatt agccctataa
    22321 taatgggaat tatactagga ggtctaataa cagtagttgg aacgtctcca cttagttcaa
    22381 tggctcttac atctataatt ggtcttactg gtcttcctat ggcaatagga gcattaggtg
    22441 cttttggttc agcttttatg aactcaacac tatttgcaaa attgaaatta ggttctaaaa
    22501 aagatattat atctgttgct attgaaccat taacccaatc agacataatt tcagctaatc
    22561 caatacctat atattctact aatttctttg gaggagcctt ttctggtatc attgtagcct
    22621 tgatgggtct tgtaaacatg acaccaggta cagctacacc tgtggctggt tttgctataa
    22681 tgtttgctta taatccagtt caacaagttc ttataacagc tggtttatgt gctttattca
    22741 atgtaattgc aggattttta ggttcaaaaa tattcagtac ctttaaagtt cttactgctg
    22801 gtgaaatacg tggtactgat gtagaagaag aaattgctta aaaaacaata agatttataa
    22861 atttatatct caagtagtag agttttatat tgtaggcaat tttattaatt gcaaacgttt
    22921 tattttttat ttatgtttat gattattatg tgtgttgaat atttagataa atttttattt
    22981 attattttta tttagatttt tgtttagttt tttatttggt tgatatatta ggaataaaaa
    23041 ttagtatagt ttattactag ttagatagca taacttttta gatacataaa aaattttata
    23101 ttaattagat attattaaaa taaaagcgat acatgaattt ttaaatgtcc atgtatcgct
    23161 ttatttttgc cataatctta acaactaatt tttaaagttt attatcttaa caaagttcat
    23221 tatcttatat aatatttaaa tgcatagact tctttaattt aaccatctaa atatttaatt
    23281 aaatattatt atatgtaata taactaattt ttaaatgaat gtataggagc tggaatcctt
    23341 cctcctcttt gagtaaataa ttcagatgaa tttgtattta taggcattac tggtgctcgt
    23401 cctaaaagtc ctccaaattc taccatatca cctacatcac atcctggggc tggtataatt
    23461 ctaacggctg tagtcttatt attaatcata cctattgctg cctcatcagc tatcatggct
    23521 ccaagcgtac ctgctgtagt atttcctggc acagctatca tatcaagacc tacagaacaa
    23581 atagcagtca ttgcttcaag cttatctata cttaaagcac ctttaataac agcatcaatc
    23641 atacctgcat cttcagatac tggtataaac gctccactaa gtcctccaac atgtgaacat
    23701 gccattactc cacctttttt aacagcatca tttaaaagtg ctaatgctgc tgtagtccca
    23761 tgagtaccaa caacttcaag acccatttcc tctaatatat ttgctactga atcaccaata
    23821 gcaggtgtag gagctagtga taagtctatt atcccaaatg gaacatccaa tctactagaa
    23881 gcttcctttg ccacaagttg tccaactctt gtaattttaa atgcagtctt tttaactgtt
    23941 tctgaaacaa catcaaaagg ctctcccttt actttctcta aagctctctt aacaacacca
    24001 gggccactta caccaacatt tataattcta tcagcctcac caacaccatg gaaagctcct
    24061 gccataaatg gattatcttc tactgcatta caaaatacta ctaattttgc acaaccaaaa
    24121 ccttttgcat cttttgttaa ttctgcagtt tcttttatga tttctcccat ttctttcact
    24181 gcatccatat ttataccaca tcttgtagaa cctacattta ctgaagcaca tactttatta
    24241 gtacttgcta gagcttttgg aattgactta attagtatct tatcaccttt tgtatatccc
    24301 ttttgaacta atgctgagaa tccaccaata aaatctatac ctaaatcctc tgcagcctta
    24361 tccaatgtct gtgcaaactt tacatagtca ttttcatctg ttgcacctgc aataatggaa
    24421 attggagtta ctgataccct tttatttaca attgggatac caaattcacg ctctatatct
    24481 tttccaacct tcaccaaatc tttagcagaa ctcataatct tattgtatat tttttctcta
    24541 gctttatctc catcagggtc tatacaatct aataaagaaa tccccatagt tatagttctt
    24601 atatccaact gttcttcatc tatcatcttt attgtttcaa gtatatttcc tacattcact
    24661 attatcacct ccccttactg tctactacag attatgcatt gaattaaata tttcttctcg
    24721 ctgaattttt acatccactg ataatttctt acctctttca accaagtcct caaccgtttt
    24781 ctcaaaagaa tttaatgatt tctccaaatc aactatcata atcatagtaa aataatcctg
    24841 taatattttt tgtgatatat ctactatgtt gatattttgc ttgtataatt catcactaat
    24901 tcctgccact atacctggtt tatcctttcc aattactgtt aaaattgctt tcataataat
    24961 atcctccctt atttttttat aaatcttata tttttacaaa ttgaactatg tgttaacata
    25021 tatttcattt atttgttagt aaaccacttt ttatgtatat atttaatata ttttaatacc
    25081 tatacattca tttgtcaatt ttttttcaat attctattat ttttatacta tttagaaaat
    25141 tcatatatta tatatgtttt ctcaacaata aatacagttt tca
//
LOCUS       DAEMIK010000047        25162 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEMIK010000047 DAEMIK010000000
VERSION     DAEMIK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.047"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            374..886
                     /locus_tag="KPK87_001635"
     CDS             374..886
                     /locus_tag="KPK87_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537790.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNVILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            complement(1824..2921)
                     /locus_tag="KPK87_001636"
     CDS             complement(1824..2921)
                     /locus_tag="KPK87_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2537791.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            3214..4740
                     /gene="glpK"
                     /locus_tag="KPK87_001637"
     CDS             3214..4740
                     /gene="glpK"
                     /locus_tag="KPK87_001637"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG2537792.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4781..5896
                     /locus_tag="KPK87_001638"
     CDS             4781..5896
                     /locus_tag="KPK87_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG2537793.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(6177..6941)
                     /locus_tag="KPK87_001639"
     CDS             complement(6177..6941)
                     /locus_tag="KPK87_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2537794.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            7200..7290
                     /locus_tag="KPK87_001640"
     tRNA            7200..7290
                     /locus_tag="KPK87_001640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7234..7236,aa:Ser,seq:gct)
     gene            7378..8166
                     /locus_tag="KPK87_001641"
     CDS             7378..8166
                     /locus_tag="KPK87_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG2537795.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            8187..8927
                     /locus_tag="KPK87_001642"
     CDS             8187..8927
                     /locus_tag="KPK87_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2537796.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            9103..9477
                     /locus_tag="KPK87_001643"
     CDS             9103..9477
                     /locus_tag="KPK87_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537797.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            9569..11029
                     /locus_tag="KPK87_001644"
     CDS             9569..11029
                     /locus_tag="KPK87_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG2537798.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAKKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            11082..14111
                     /locus_tag="KPK87_001645"
     CDS             11082..14111
                     /locus_tag="KPK87_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2537799.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            14188..16254
                     /locus_tag="KPK87_001646"
     CDS             14188..16254
                     /locus_tag="KPK87_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2537800.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQNEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    16917..17127
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17353..18168
                     /locus_tag="KPK87_001647"
     CDS             17353..18168
                     /locus_tag="KPK87_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2537801.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            18196..18873
                     /locus_tag="KPK87_001648"
     CDS             18196..18873
                     /locus_tag="KPK87_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2537802.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            18932..19654
                     /locus_tag="KPK87_001649"
     CDS             18932..19654
                     /locus_tag="KPK87_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2537803.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            20302..21453
                     /locus_tag="KPK87_001650"
     CDS             20302..21453
                     /locus_tag="KPK87_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG2537804.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            21499..22674
                     /gene="thiI"
                     /locus_tag="KPK87_001651"
     CDS             21499..22674
                     /gene="thiI"
                     /locus_tag="KPK87_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG2537805.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            22850..23653
                     /locus_tag="KPK87_001652"
     CDS             22850..23653
                     /locus_tag="KPK87_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2537806.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            23896..24537
                     /locus_tag="KPK87_001653"
     CDS             23896..24537
                     /locus_tag="KPK87_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG2537807.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT        10058 a         2540 c         4509 g         8055 t
ORIGIN      
        1 agtaaaatga ttttgatata ctctctatat tttgtatgtt aaaagtaaat aaattattgt
       61 taaagaaggt aaagactttt aaatttctta tcataggatt ttaagtaaat aagaaggatt
      121 ggagtaatta aaaattttta ttttgcaaaa ataagtattt atttatatgg ataaatttga
      181 ttttaaatct ataaatatag ataaaatcta attatttaac ttcaactatt cctcaaaaaa
      241 gcaactttta aggaatagtt gaagcaaaaa gtttggttac ataatagtaa ttttagttac
      301 aagaatgaaa ctgtaaaact taggattttc tttatataat ttcattgaga acatttaatt
      361 taggaggtaa gatatgaaag taaaaaaatt taaaagtggt atagtaatgg cattaacagt
      421 aatggcaatt ggagcatcat tgacaaatgt atcagctatg gaattaaaca agagtaaaga
      481 gtttgaaact aaatacaatg aaatgaagag ggattttcaa gaggaatatg gaaaagaaga
      541 caaatttaat ataacattgg atgatgaatt tacaaaagaa gaagtaaatg gagctaaagg
      601 gattataaag acaactaata aaatgaataa tgaggttgta gtattaaatt attttgatga
      661 aatgaaatct ggaattgaat ttgagttgaa gccaaaagca gaaaaaaatg taattttgag
      721 agatgaattt caacaaggtt atggaaagga agataaattc aaagttgtgt tagatgatga
      781 atttacaaaa gaagaggtaa atggagctaa gggcattata aagactacag ataaagagac
      841 aggtgaggtt atcgtatata attactttga tgagcttgaa aaataatgct taaagattac
      901 tttgattgaa tgaaaaggta attgtgttga gaatattaaa tgactgaata aaaacagttg
      961 tatcaaaaac aaacaataat agaatgtaag aatgattgtg tcgaaaatat aaaatgacta
     1021 aataaaaaac gattgcgtca aaaataaaca ataatagaat gtaagaataa ttgtgtcgaa
     1081 aatataaaat gactgaataa aaacagttgt atcaaaacaa acaataatag aatgtaagaa
     1141 tgattgtgtc gaaaatataa aatgactgaa taaaaaacga ttgcgtcaaa aataaacaat
     1201 aatagaatgt aagaatgatt gtgtcgaaaa tataaaatga ctgaataaaa aacgattgcg
     1261 tcaaaaataa acaataatag aatgtaagaa taattgtgtc aagaatatta aatgactgaa
     1321 taaaaacagt tgtatcaaaa acaaacaata atagaatgta agaatgattg tgttgaaaat
     1381 attaaatgac tgaataaaaa acgattgtgt caaaaacaaa aaataattaa gtgtaagaat
     1441 gattgtatca agaatataaa atgattcaac aaaaaacgat tgtgtcaaaa ataaaaaata
     1501 attaagtgta agaatgattg tatcaagaat ataaaatgat tcaacaaaaa acgattgtgt
     1561 caaaaataaa aaataattaa gtgtaagaat gattgtatca agaatataaa atgattcaat
     1621 aaaaaacgat tgcgtcaaaa atataaaata aattaaaaga aaaagattat attgaaggaa
     1681 aaagacttga aataggggtg aactttacat attatagttc acccttatac aaaaatataa
     1741 aatataattt attataggaa aatttaaaaa aatcagtcta tttaatttat actgatatat
     1801 tgtaggattt tacaaacaaa ctgttatata acttttttta tctccaattt tgattttaag
     1861 atatctaaga atttataaca atcttctgga tatatttctc ccatatttgc tacttggaat
     1921 atgttagcat ctaggaattt gccttttcct ggatagacaa catagccact ttccctttct
     1981 agccctaata taaactcatt tacattgatt tctttaggta gaaatacaga tgttactgta
     2041 ttagacatat acttatctgg aagtaaaaat gttagaccca attcttccat accattcctt
     2101 aaaatagcag aacattcttt gtatctttct attttactgt catcttccaa aaattcagtt
     2161 aaagcttcat taagggcaaa tattaaagta acattaggtg tatttggtgt ttgagaagat
     2221 gatttagcaa ctttatagtg tttataaaga ttaagataca cattcttaca ttgattttct
     2281 ttaatatttt gtaaaatact ctctttagca caaaggtatg cagaacctgg aaatgctcca
     2341 agagctttgc caccaacacc tgttattata tctatattaa actcttccac atcaatatat
     2401 tcacctgctg aggcacttat tgcatctaca aaaaatattt tgttatactt tttagcaagt
     2461 tttcctagtt cagggacggg atttatcatt cctgaactag tttcatggaa caccatagca
     2521 attactttta tatcagaatt ttcaattatg tatttttcta aaatgtctag gtccgggtat
     2581 tctgcccatt caaaattagg tttatatgtt tttacattat aagtagataa tatttcttct
     2641 agcctttctc caaaaacacc attactaact agcagaactg catcttcttt gtcaaagata
     2701 gaagatagta cacattcatt tacagatgtt ccagagccag atacaattaa tgagtagtaa
     2761 ttagaagttg cattatatag tttattgatt ttttgttgtg tatctttgaa taaatccata
     2821 aactcatttc ctctatggca tatatcatag tgaagaagtg aatttctgac tttatctgta
     2881 accattactg gtccaggact gaatagatat gtttttttca ttgtaaagcc tccttaaaaa
     2941 gttaaattta aatttttgaa tgactgatta tgtgtaaata ttaaaatgtt atgtgttatt
     3001 ttaactagat tatagaataa aataatgaat attgcaataa tgattcaata aaaatacttt
     3061 tttaatcact ataattaaaa ataacaaaaa atattgtaat gaatattgca taaatccaca
     3121 tgatgatata aaatataaat aacttgaata gattatatat tcaaaaaata aaaaagaatt
     3181 tattaattct gaaaataaag ggggacattt aaagtgaaat atgtattagc aattgaccaa
     3241 ggaacaacag gaacaagagc agtattgata aacgaaaaat cagagtttgt ttttagcgat
     3301 tatatggaac atactcaaat atatccacaa tctggatatg ttgaacatga ccctatagaa
     3361 atttggaata atacaaaaac tgtgagtaat aatgtattac aacaggcttt tgaaaatgga
     3421 atcaatgaaa gtgatataaa aggaattggt atagataatc aaggtgaaac agtaatgtta
     3481 tgggataaaa atacaggtaa accactttac aatgctattg tgtggcagtg caggagaact
     3541 tatgattatg tggaaaattt aaagaatata ccaggtttag aaaataagat aatagataag
     3601 actgggttaa ttatagaccc atatttctca ggtacaaaaa ttaaatgggt aatagataat
     3661 gtaaaaggtg taaaagaaaa aattaaaaat ggagaagtac ttgcaggaac attagatagt
     3721 tggttaatat ggaaaatgac aggaggaaaa tcttttttaa ctgatgtatc tacagcagca
     3781 agaacaatgc tttttaatat aagagatatg gattgggata gagagttgct tgacattata
     3841 ggagtaccta gaagtatttt agcagatata atgcctacga gtggtgattt tggaactaca
     3901 gataaggagg cattttgtgg ggtttctata aaaattactg cttcaatggt agaccaacca
     3961 gcagctttat ttggcaatgg atgttttgaa ccaggaatgg ttaaaaatac ttatggtaca
     4021 ggatgtttcc tatatatgaa tataggtgag gaactaaata tagcaggtaa tggtattttg
     4081 acaactgttg cttggaaaat aggagataaa gttacatatg cttatgatgg tggagtgtat
     4141 atagcaggtg ctgcaataca atggcttaga gatggcattg gagttataaa aaattattca
     4201 gaaactgatg acatggcaaa ttccatatca agtactggag gtgtatattt tgtaccagca
     4261 tttgcaggta tagcagcacc ttattgggac caatatgcta gaggtacaat ggttgggata
     4321 acaggaagta ctaaaaaaga gcatatagta agagcgactc ttgagtctgt tgcacttcaa
     4381 gtcaaagatg ttgttgattc tatgaattta gcctcaggga aaaaaataaa aatgttgaga
     4441 gtagatggtg gaattactaa aaataacttt acaatgcaat ttcaatccga catattaggt
     4501 ataccaattg aaattgcaga aaatcctgag acgacagtac ttggagtagg atatatggca
     4561 ggtctttctt gtggtgtctg gaacagtatt gatgaaatta gaagtgaatt tacaataagc
     4621 aaaaaatata ttccaaatat gactactcaa aaaagagaag aaatattact tggatggaac
     4681 caagcagtaa aaagagctat gaattgggaa aacgatacca aaaaggtgtg tgctaattaa
     4741 gttacaatag agtattttac atgtatagga ggaattaaaa atgccctttg ataaaattat
     4801 attatacata atggctgtgg gagtgctttt aggagcgtta gatagaataa ttggaaataa
     4861 atttggactt ggcgagcagt ttgaagaagg gtttaattca atgggtccat tggctttagg
     4921 aatggttggt atagtttgtt tagctccagt aatttcagat gtactaggtc ctataataat
     4981 accaatcttc aatctctgtg gagctgaccc atctatgttt gcaactattt tagcgaatga
     5041 tatgggaggg tatccattgg ctatggaact tgcacacaat aaagaggcag gaatgcttgc
     5101 aggattggtt gtgtcatcca tgcttggatg tacattagta ttttcaattc cagtaggact
     5161 aggtcttata gaatatgaag acagaccatt ctttgcaaaa gggttactga taggcttgat
     5221 aacaatacct tttggaggta ttataggagg attaatagca ggatttaact tatcaatggt
     5281 aattataaat atgataccag tgataatatt ggcagtaata ttggcagtag gtttaaaatt
     5341 taattctacg cttatgataa atgggtcatt agtttttgga aaatttatat ctatattgat
     5401 aacgataggt ttgggagcag cagcatttca agaaataaca ggtatagtac ttataaaagg
     5461 catgacacct ataagagaag gtattcaagt aattggaagt attgcaatcg tgttattagg
     5521 tacttttcca atattacatt tattagttaa agttcttaat aaaccactta caatagtggg
     5581 tggaaaatta ggtatggatg ccactagtgc agcaggatta gtttttactt tagccaatag
     5641 tattccagta tataaaatga tgaaagatat gagtccaaag gggaaagtag taaatactgc
     5701 ttggctggtt tgtgctacag cagctttggg ggaccattta ggatttacag caggagtagt
     5761 tccagagatg attacacctg ttgtaattgg aaaattggtt ggaggagtat tggcaattgc
     5821 acttgctata cttatgacaa aagatttaac cttggaagaa gaacaaagta ttaaaatgaa
     5881 gaaagatgca gtgtagaata aacaatactt aagtcaaatt taaattattt taaagtagga
     5941 gtttaaataa ctaaaaataa attaaaaaga cagacaatta aaaaataatc gaataactaa
     6001 aacttataaa ttaaataaaa tggcaatcaa ataactaaaa atgataagct aactgaaagt
     6061 agttacataa ttaaaatata gagtatagtt tagacataaa tggagctaaa taaataatta
     6121 tttggctcct taattttaat ttattacatc aaggaactaa aaatcaatca tataagctaa
     6181 ttatcagcta tgactaaagg tattcctttt tgagaaaact ctattattat attatggtct
     6241 atactagcag aggtaataat agtatctata tgttctatag gagctactga tgcaaatgaa
     6301 tttataccaa acttaggagg ttcgacaact actatagatt ttctagacct ttcaatcatt
     6361 ttttttctaa caagtgcttc ttctaagtga tagtcagaga ttccattttc aagagtgata
     6421 cctccagcgc ctaaaatagc tttatcaaca ttaaattgtt caatagctga cagtgtaagc
     6481 ggaccagaaa aagaaccttc agtttctcta agatagcccc caagcacata tacttttata
     6541 ttagtattca taagttcgtt tgcaactaaa attgagttgg ttattacagt tagattcttt
     6601 ttatctttca aattttttga aacttcaagt acagtagttc cagtatccat aaaaatagta
     6661 tcatttgtat ttataaactc actacattta atagcaatct gttttttatc gtttgagttt
     6721 attttttttc ttttaacata gttaagttct acgctacttg ctataactgg tatagcacca
     6781 ccataaactc tttttaaaag accgatactc tccatatggg ctaaatctct tcgtgcagtt
     6841 tctattgata tatcaaacat ttgaactatg tcatttattt taattatatt atctgatttg
     6901 agaatactta aaatcttctc atatctttcc tgtggtaaca taaaatatcc tccttggact
     6961 agtattattt tagttttggc ataacttaca attaagtcag agtttaggta tatttgttaa
     7021 tgtatgtata tttatcaaat aatattataa gtgttatgca agataatttt accatattta
     7081 aaacttacat ttaagcaaaa aagtaaaaat agatgtttaa taattcaaaa taactcaaat
     7141 tttataaaac aattgactaa aatatgattt tattatataa tttactagta tagagatatg
     7201 gagaagtact caagtggctc aagaggatcc cctgctaagg gattaggctg ggtaaccggt
     7261 gcgagggttc gaatccctcc ttctccgcca attattaaag gctattttta ttaaagatag
     7321 tctttttata atgtcataaa tgattagata tgtataacta aagaaaggag ggaatagatg
     7381 acaactttaa tttttttcat agtggggata gcatctttgg tagttgcttt tgttattgat
     7441 gggggacatg ttggagcatt atttgtattt tcatcagcac ttgttgtgtt tggtggaact
     7501 ataggagctg ttggagcttc gactccattc cctgtgttta aaaagtctat aaagataatg
     7561 ttattagctt ttaaaaataa aaagcctaat aacatagaaa atataatcta tttcaaagaa
     7621 attgcaacaa aggctaggaa agaggggctt cttagtattg acaaagaaat aagtgagaat
     7681 gatgaattag acccttttat tagaaaaggg cttgaactta tggtagatgg agtggatgca
     7741 gttactataa gaaatatatt ggaatcacag atacagataa catcaaaacg acataaagaa
     7801 ggaatagcaa tttttgattc agcaggggga tatgcaccta cactggggat aataggtaca
     7861 gttatggggt tagtacatat tttaggtaat ctttcaagta atccagatgc attaggacct
     7921 caaatttctg ttgcattctt ggctactttg tatggtgttg gttctgccaa tttaatatgg
     7981 ttaccaatag catcaaaact taaggctata aatcaacagg aaataagtga aagacttttg
     8041 gtgatagaag caatattatc catacttgaa ggtgataatt ctaatttaat gacagaaaaa
     8101 ttaaaaatat ttttaaattc aaaagaacta attgaacttg aagaaattgg tggtagagct
     8161 gaataatata aaaggatgga atgtttatgt tgtatgatga agatgaaaaa gaagaaaaca
     8221 atgagcgatg gcttttaact tattcagacc tcataacatt acttatgatt ttctttgtaa
     8281 taatgtattc tatgagtaat gttgatgcgg agaaatataa acaattatct caatctctaa
     8341 attctgcttt tggtggttct tctggagtta ttgaaggtgg taatagcaag atagaaccag
     8401 tagttgaacc aggaagtaat gatttagata ctttacaaaa tgctaagttt aaaaaagttg
     8461 gtgaagaaat ccagaagtat ttaaacgaaa atggtatggc taattcagta tcattaagag
     8521 ttcaagatag aggattagta ataagcctta aagatacaat actatttgat acagggaaag
     8581 cgattgtgaa ggataattct agagataaga ttattcaaat aggtaagatg ttaaatgaaa
     8641 tgaatagtta catgagagta gaaggccata cagacaatat gtctataaaa aattcagagt
     8701 ttaagtcaaa ttgggattta tcagttatga gagcaaccaa tgttgttcaa cttcttattg
     8761 acaatgcagg tatagcacca gataaattat ctgctgtagg atatggtgag tttagaccaa
     8821 tagcagaaaa ttcatctgaa gaaggtaggt caaaaaatag aagagttgat atagttttag
     8881 ttgatagtaa atatgataat gtagaaaatg tttcaaagga taaatagtta aattagctgt
     8941 ctcaatacag aattaattct aaaggagatg gcttttttat gcgaaaatca aaaatagata
     9001 catagtaagt tacctttttg taggaatata tggtatctta aatatataat atttaaataa
     9061 tgtataagct caattaaaaa atttatagag gatggaaaat aaatgggaaa taaaaataaa
     9121 aatactaaat ataaagataa aacaagagaa aggtataata aaaaagttga gaatttaaat
     9181 aaagaaaaat taaataaagt taataagcaa tctaagaagg tagataagat tgatgatatt
     9241 attaataaat ctagtattgt attatgtgta cttctagtag tgttttcatt gctttactta
     9301 tttaaactgg ttcaacttaa tataataatg atacttttag taattacagt agctattcaa
     9361 caaggattgg tgtcatttgg tgcatttaga aataaaaatt atgtaaaagc tttattttct
     9421 atatttgtaa tagtatgttt cttaattgct tttaaatatg ttgtaaatcc tgtttaaata
     9481 tatcaataaa cgttctataa ttaatataaa tagtgaagtt acagttttaa tgctattata
     9541 aattttataa tttgaaggag tagattttat gaataggtgt aagtttgcaa aattaattat
     9601 cacatcaatg attgttttat tacttacagg atgtagttta aagaaggaag ataattataa
     9661 gttagaccca gaaaatccga catcaataca aatttggcat tattataatg gacagcagaa
     9721 aatggctttt gataagcttg ttaaggaatt taatgacact gtaggtgcta aaaaaggaat
     9781 tattgttgaa gcattcagtc aaggtaatgt agttgattta tcagagaaag ttttagattc
     9841 tgcaaataaa aaagttggtt ctgaagatgt accaaatata tttgcagcat actcagatac
     9901 tgcatatgaa atggataaat tagggttact agtagatatg gataagtaca taagtaaaga
     9961 agaattaaat gaatacatag acgaatatgt agaagaaggc agaataaatt ctaaggatga
    10021 gtttaaactt ttcccaattg caaaatcaac tgaaattttg atgttaaata aaactgattg
    10081 ggataaattt gcaaaagcta caaattcaaa tataaatgat ataaaaacaa ttgaaggatt
    10141 agtagagaca tcaaaaaaat attatgaatg gacagatagt ttaacgaaag agcctaatga
    10201 tggtaaagca ttttttggaa gagatgcttt agcaaattat attattatag gaagtaaaca
    10261 actaggagta gaaatttttt ctgttaaaga tggtaaagtt acactaaata ttgatgaaaa
    10321 agttatgaaa aaactatggg ataatttcta tgtaccatat ataaatggat attttgcttc
    10381 ttatggtaag tttcgttctg atgatgcaaa aacaggtgat atattagctc ttgtaggttc
    10441 tagttcagga gcagcatact ttccagatag agtaatgcta aatgataatg agagttataa
    10501 tattgaaaca tctgtttttc aagcaccacg ttttaaagga gaaggaaaat atgctgtaca
    10561 acaaggtgca ggaatggctg taacaaaatc tactaaagaa gaagaatatg catcagtaga
    10621 atttttaaaa tggtttacag ataaagaacc taatataaaa ttctctattg cttctggata
    10681 cttaccagta aagaaaaaaa cgaatgattt ggatgttata aaagaagtta tgaagaaaga
    10741 taatttgagt ttatctgata aagttgaagg ttcaatagcc tcatctattg aacagattaa
    10801 cagttgtaat ttatatacta ataaagcatt tgatggtgga actaaagcta gacaaatact
    10861 tgaatattca atgcttgata aagctaaaga agattcaaat aaaataaatg aattagtaaa
    10921 aagtggaact tctaagaagg atgcagtagc acaatttaat aatgataaaa attttaaaac
    10981 ttggtttgaa agttttaagg ctgagcttga aaatgctata aaaaaataga tgaattggca
    11041 gttagtgtgt tgacgagcac aaaggtatag gtggaatggg tatggaaaac aatctaagaa
    11101 aaaacaattc aataatgaaa aagatgatat ttcccctaat tattgttatg ctaattcaaa
    11161 caagtttatt ttgtgccaca attttatggg gaggaactat taagaaatta aatgataatt
    11221 cgtttgacat cttaaatgaa agagtaatta atcgtaaaaa ttatatacaa aatgaaatgt
    11281 tacaaagatg gtcaaataca agtgaaacgg aagctacaat taattcttct gtaaaaaaag
    11341 tattaaatga aaaaaatgcc ttaattgagg atgtaggcaa caatgaagaa attaacaaag
    11401 atattataaa aagcatatca aatgatttaa tatatctttt gagaaaaaat tctgttacag
    11461 gagcttttat aatcctaagt gacaaaaata ttaacgataa gtcaatagat agtaaaggtg
    11521 aaataagtag gacaggattg tatataaggg atttagaccc taagtttaat ccaaatgata
    11581 attctgattt gttaattgag agaggttcat cagatatagc aagaagtttt ggaatatcta
    11641 tggacagtta ttgggaacct aagttttcat ttaaaagtga aaatgagcaa gatggagatg
    11701 atttttttta taaaccattt agagctgcaa ttgataatcc aaatgtatca agttctaatt
    11761 taggatactg gggaagtaag tttttattga caaatgcagg tgataggctt atgacatata
    11821 cagttcccct tatttatgaa gatggaactg tatatggagt acttggaata gatttaagtg
    11881 taaattattt aagagaatta cttccatatt cagagataaa tgggaataaa aagggtaatt
    11941 atattttagg tgtagataga aataatgata tggcctttga aaatgtaatt tcaaatggat
    12001 actcattaaa agaaaaattt tgggatggtt tacattctaa tttagagcag aaggaatcgt
    12061 ataaaaatat atataagatt aaaaataaag ataatgaatg tggtaatggc aacatttatg
    12121 ctagtgtaca atacttagat ttatataact ccaatactcc atttgaaaat gaacgttggg
    12181 ctcttttggg tattattgaa gaaaaaagtc ttttgtcatt ttcaaaaagg gtagagaaat
    12241 tagttataat atctatagca atttctttat taataggtct aataggtatt tacattgcat
    12301 caagattatt tacaaaacca attgttcttt tagccaacaa agttagaaac agtaatccat
    12361 caaaaccagt aaatcttgat aagataaata tatctgaaat agatgaatta tcttcagcta
    12421 tagagttgct cagtgcaaat gttgcagatt cttcgtcaaa attatcacag attattgaaa
    12481 tggtagatat gccaattggg gcttttgagt tatacaaaaa cgcagagaat gttttttgta
    12541 caaaagaatt ttttagcgtg tttactactg aaatcaaatt tgaagaatat ggatatatgg
    12601 aaaaaaataa gttcctgaaa aaattaaggg gactagataa atatttgcaa gaaagttatg
    12661 aaacagaaaa tacatatata tataagtttg aatctaaaga taaaaaactt aagtggataa
    12721 gaatgaatat ctttgaagat gagctaaagg ttcttggtgt tgttgttgat gtcacaaaag
    12781 aatttattga gaaaaggaag atagaatatg aaagagattt tgatatttta acgaatcttt
    12841 taaataggag agcttttcat tcacagatac aagaaaaatt taagaataaa gatgaactta
    12901 agatagcagc atttattatg tgggatttag ataatctaaa atatattaat gatacatatg
    12961 gacatgatgc tggtgatgaa tatattaggt gtgctgcaga catacttaag aagtttatat
    13021 catggaatgg tatagtatct agactttcag gtgatgagtt ttatgtattt atatatggat
    13081 atgaaagcaa agatagtata agagatatta ttaattctgt taagaaacag atgaataata
    13141 ccttattaaa acttcatgat ggaacagaat ttaaaataag ggcatcagca ggaatttcct
    13201 ggtaccctga tgactcaacc aactatgatg aattaatgag atacgcagat tttgctatgt
    13261 atcaaattaa aaatacaatc aagggtgaag ttagtgaatt tgatattaat atatataata
    13321 aggattcatt cttattgagt aatagagaag aattaaacaa gttaattgat ggtcaattag
    13381 ttgagtatgc atttcagcct attgtagatt gtaaaggtaa agtatttgca tatgaagcac
    13441 ttatgagacc taaaataaaa acttttagtt caccaataga tgtcattaga ttagctcgtt
    13501 cacagtctaa attatatcaa attgagcgta ttacatggtt taaagcactt aaatcattta
    13561 aaaaatacaa agaaaaattt ggcgattgca aactgttctt aaattctatt cctaataata
    13621 atttgtcaaa agtagatttt gaggaaattg aagatttata tggtgaatat ttaaatagag
    13681 tagtactaga aataacagag aatgataaga ttaatgaaga ttttattgag aaaaagaaag
    13741 catatataga ccattggaat atagaaattg ctttagatga ttttgggaca ggttacaacg
    13801 gagatgctgt actattatat ataatgccag actatgtaaa gattgatatg tctattgtaa
    13861 gaggtattga taaagatgaa aacagacaga aaatattaaa aaatcttatc tcatattcta
    13921 acgaaagaaa tattaaagtg atagcagaag gtgtagaaac aaaaggtgaa atggaaacac
    13981 taattaatct tggagtagat tatatgcaag gctactatat tagtaaacca tcatttatac
    14041 ctgaagatat aagcgacaaa attaaaaaag aactcaagat atgtcatgaa aataggaatg
    14101 atgtggttta atagaggaaa aattaaattt atttttatga acttttagtt tataattaaa
    14161 aagaaaataa tataattata aatcaaggtg gtggagctaa taatcacaac aaatatagat
    14221 ataagaaagt taaatgaaaa tcaattagaa gcagttgagc atatagatgg gccttgcatg
    14281 atacttgcag ggcctggttc aggtaagacc agagtaatta catatagaat agccaatatg
    14341 gttgtaaaca aaaacatagc accaacaaga atcttggcaa taagttttac caaggcatct
    14401 tcaatagaaa tgaaaaatag agctttgaat ttaagtgatg atataagact taataaggta
    14461 acctatggta cttttcactc tgtatttttt aaaattctaa gatactttga aagatacaac
    14521 ttagacagta tatttgatga aaagtcaaag cgaatgacaa tcaaggcaat cctaaaaagt
    14581 ttaaatatag agaatgctga tgatgatgag aatataggac aagttataaa tgaaatttcc
    14641 tatgtaaaaa atgaacttat ggataaaaat gaatttaatt cagaagtatt aacaaaagat
    14701 gagtttttaa aagtatacaa cttatacgaa gaacaaaaat ctaaagttaa taaaatagat
    14761 tttgatgata tgcttataag aacttattat ttacttttga acaataagtc tgcactagaa
    14821 atggttagaa atgtgtataa atatatattg atagatgaat ttcaagatat aaataaagta
    14881 cagtttgaag ttttaaagtt aatttgtagc ccattaaaca atatatttgc agttggagat
    14941 gaagaccaaa gtatatatgg atttagaggt gctagaccag attttttgct tgaatttgaa
    15001 caatacttta ataatactaa aaaaataata ttagatataa attacaggtc taaaagtgag
    15061 atagttcata ctgcaaatag acttatcgac aaaaataaaa atagatatga aaaaatcatt
    15121 aagtgtagtc agggggatgg aggaagtgtt acctatattt ctccgcatga ttcagaggaa
    15181 gaagccttat atattgccag ggaaataatt gacgaaatac aaaaagatta tgtagagtat
    15241 tcagattttg cagtaattta tagaactaat atacagtcaa gagcattagt agatgtattt
    15301 atggatatga ggattccatt tgtagtaaaa gactcagtaa ttacaatata tgaccattgg
    15361 gcaagtcaag atatactagc ctatttaaga ataggtatta atccaaaatc aaataaagat
    15421 tggttgagaa ttataaacaa gccttttaga tatatatcca aagacagtat aaatatggtt
    15481 aaggatgaaa aagactttat aacagcatta ataaataagt gtaatcttca tcctaaacaa
    15541 gttaaaacaa taaatgattt ggaaatagat ttaagttatt taaatacttt aaatcctaaa
    15601 aatgcaatat cttatattag aactagtctt gattatgata gatatgtttt agattattgt
    15661 agcaacagaa agataaagac taatggactt gtagagatat taaatgaact tgagagttca
    15721 gcaactaatt ttaatactgt tacagagttt ttagagcata ttgatagagt aaagactgag
    15781 ttaacggaaa ataacaagaa taagcagaat gagggtgtca tatttacaac tatgcatagt
    15841 gcaaaaggat tagaatttag aaatgtttat attataggtg taaatgaagg aacaatacct
    15901 catgaaaaat catatgatat ttgtaaggaa gagaaaaaag aagaacaatt agaagaagaa
    15961 agaagactta tgtatgttgc tattacaaga gctgaagaaa agttatgtat aagctctact
    16021 ttaaataagt atggtaagaa agttgataaa tcattattta ttaatgatat aaaatctcca
    16081 acgaaaaaag aaattgacag tattggtgta ggggataagg tataccataa aaaatttcat
    16141 gaaggagaaa taattaaaaa agatggaatt atgtttacaa taaagtttaa agatagagag
    16201 cgaatcttgg atttgaaaac ttgtttattg aagaatataa tttatactat ttaatttaga
    16261 tagaaatata atgtattatt tattatgtgc aagacaaata gatatgtgta aaatgtatta
    16321 tttaacttat tagaaaaaag aaataagggt ttaatatatt atttatagtt gtttagaaaa
    16381 agcaacaatg tggaaaatat tatttagtat tattaaagaa aagcaacaat gtgaaagata
    16441 ttatttagtg taatttaaga aaaagcaaca gtgtgaaaga tactatttag tattattaaa
    16501 gaaaaagcaa cagtgtgaaa gatattattt agtgtaattt aagaaaagca acaatgcgaa
    16561 agatattatt tagtattgtt taagaaaggt caacagtgtg aaagatacta tttagtatta
    16621 ttaaagaaaa agcaacagtg tgaaagatat tatttagtgt aatttaagaa aagcaacaat
    16681 gtgaaagata ttatttagta ttgtttaaga aaaagcaaca atgtgaaaga tattatttag
    16741 tattatttaa gaaatagcta aaaatatgaa atatactatt tataattaca caaataatat
    16801 caataaatat aaacagtagt atttagataa tttacaagta aaaattatta aaaaaataaa
    16861 aaacttattg acaaaaatat tataaggaag tacaattgta ttaacaattt aaggaaacaa
    16921 atccaatgat agagaaagta gcatatatgg atttacagcg agttaggggt tggtgtaagc
    16981 ctagcaatga agtctatgtg aagagagctc tggaggagat tatcagaaac tttagtatga
    17041 taattggttt acccgcctta agggattgag aggataagta cttacttatc aaaaagagtg
    17101 gtaacgcgga tataattcgt ctcttagctg taaagctaag ggactttttt gatttaaaaa
    17161 taaaaatatc catacagctt aaatgctaac agtcacaaat tttgaaagta atataagtat
    17221 ttatcaataa gagtggtaac gcggtaaagt cgtctcttag tcattaaaga ctgagagact
    17281 ttttttgtaa ctcgaaacat ttaagtatta aaatctgagg gaaattataa ttaaaagaga
    17341 ggggtagtta aaatgaaagt atttaaaaaa ttattaagtt taggattagt attaggattg
    17401 acattatcgc tagtaggatg tagtggtgga ggagaaaaaa caaagttaga acaaataaaa
    17461 gacaatggaa aattagttgt tggtactagt gcagaatttc ctccatttga atttcataag
    17521 gtagtagatg gaaaagattc aataaaagga ttcgatataa tgttggctga agaatttgca
    17581 aaagagcttg gtgtaaaggt tgaaattaag gatatgtcat ttgatggatt aataggtgct
    17641 ttaaatgcag accaagttga tattgttctt gcaggtatgt ctccaacacc agagagagaa
    17701 aagagtgttg atttttcaga attatattat ttaagtagaa atgcagttat agttaaagat
    17761 gcagatatag acaaagtaaa aactgaagat gatttaaaga aacttagagt tggagttcaa
    17821 gcaggaagta tccaagaaga atatgtagtt aacactttaa aaatgacaac tacaaaatct
    17881 ttgaaagcaa tacctgattt aattacagaa ttaaaaaatg gaaacataga tgcagttgtt
    17941 actaatgaag cagtttcttt aataaatgtt aaaaaatatg atggaataaa aatggcaaat
    18001 actgaagttg ggaaagatgt aacagaaggt atggcagcag caatcaaaaa atctgacaat
    18061 aacaaagatt ttatagaatt attaaacaaa aagataaaag aattacaaga tggcaagaag
    18121 atagaagaat ttttaaatga agcatctact gaagctgctt caaattagta agaaagttat
    18181 gtgggagatg aaattatgag tttagatttt agttttttaa gtagatttgg gacttctttt
    18241 ttggaaggaa caggtgtgac agtatcaatt tctcttgtgg cattatgctt tggatttata
    18301 ataggtataa ttatctgtat ggcaaaaata tcaaagagca aagtattaag agcaatatca
    18361 tcaatctata ttgaggtttt aagagggaca ccattattag tacagatata tatagtatgg
    18421 tttggtttac ctcaattagg aataagattc cctatgttgt ttggtatacc aagtgaattt
    18481 atagctagtg catttgcatt atcagtaaac tctggtgcat acgtggcaga gatacttaga
    18541 tcaggtatac aatcagttga taatggacaa atggaagcaa gtagatcatt aggattaaat
    18601 tattggtcaa ctatgagata tataataata cctcaagcta taaaaaatat attaccttca
    18661 ttagcaaatg agtttattac acttgtaaaa gaatcttcaa taatatctgt aataggagtt
    18721 gttgagataa tgcgtactgc tgatatagta aaaaatgcag catttagagc attagagcca
    18781 ttaatagtag cagcagcaat ttactttgtt ataacattta ctctatcaag attagttgga
    18841 ttattagaaa agaaactatc tgtgtctaac tagatgagag catgtttagc atttagggaa
    18901 aacatagaat agtaaaaagg aaagtgataa tatgattaca ataaaaaatt taagtaaatc
    18961 ttttggagat ttaaatgtat taaaaaatat agacttagaa atcgctaaag gcgaaataat
    19021 ggttatagta ggtccaagtg gttctggtaa aagtacactt cttagatgta tgaatttact
    19081 agaaatacct acaggtggag aaataatatt tgaaggaaaa aatttggtcg ataaaaaaac
    19141 taatattgat gaggttagac aaaatatagg tatggtgttt cagaacttta atttattccc
    19201 acataagact attttagata atataacatt agcaccaatt aaattaaaaa agatgactaa
    19261 ggaagaagca gaaaaaaaag cagaaatttt gttgagtaga gttggtcttt tagataaaaa
    19321 agattcatat ccatcacaat tatctggagg acaaaagcaa agaatagcaa tagctagagc
    19381 acttgctatg gaaccagata tgatgttgtt tgatgagcca acatctgctc ttgacccaga
    19441 aatggtaaac gaagttttgg atgttataaa agaacttgct aaagaaggta tgacaatggc
    19501 aattgtaact catgagatgg gatttgctaa ggaagttgca gatagagtta tttttataga
    19561 tggaggaagt atacttgaag ataatactcc agaagaagtt ttcggaaatc caaaacatga
    19621 aagaacaaaa gcgtttttag caaaagtgct ataatatata aaaaggatgt tattgatatt
    19681 agaaattcaa tacatccttt tttagtgatg gttaatatta attttaaaat taaagttata
    19741 ttaatattaa ctttattttt acgatttttt gcagaaaaaa aaagagctcg gggtaagctc
    19801 tttttttcca ccgaattaat ttccgttact aatccgattt ttaataaata taaaatatat
    19861 ccttatgaaa gggtattggg aatagatata ttaactaact agggttacca gggggataac
    19921 cttaatttat tataacaaaa tgcgacacaa aaatcaatat tttttttgaa ataaattata
    19981 aaagttattt ttataacaag taagcttata aatttaactt ttatagtaaa aataagtata
    20041 tgtataatat aatatattta ttttatataa tatattattt tatcatatat tgtattttat
    20101 aatatatttt tgtagggtat agtataagta tattatattt tactataaaa atttgtaata
    20161 aagtgaaaaa actagctttt catgaaataa tattttataa tttaagattg tctttttaat
    20221 aaagattaac aaaaaaaata ctataattag tggtataata gttagagttg aagattttaa
    20281 ataaaggtag gtgtaattaa gttggaaata tacttagaca atagcgcaac tacaaaacct
    20341 tatcaagaag ttatagataa aatggtttat gcacttaata cggaatatgg aaatccttct
    20401 tctgtccata gaaaaggtgt agaagttgaa aaagctataa aagaagtaag acaagatata
    20461 gctaaatctc taggagcgaa agaaaaggag atttatttta catcaggtgg tacagagtgt
    20521 aataatacta taataagagg tattactagt ttaaataaaa aaagaaaaaa tcacataata
    20581 tctactaaca tagaacatcc ttctgtttta aatacattaa aagatttaga agaagatggt
    20641 tttgaagtaa cttatttaga ggtcggaaaa gatgggaaaa taaatataga ggatttaaaa
    20701 aatgcgataa agtcaactac atgcttagta agcatgatgc atgttaacaa tgaaatagga
    20761 actatacagc caataggtga agttggaaag tatttgaagg gtttaaaaga aaaaatttat
    20821 ttccatgttg atgctattca atcatatgga aaaattaact ttagaccatc taaatacaat
    20881 atagatttta tgagtgtaag tgcacataaa tttcatggtc caaagggaat tggatttatg
    20941 tatataaaag aaaataatcg ccttaagcct atgttaacag gtggtggtca ggaaattgga
    21001 attaggtcgg gaacagaaaa tgtacctgga atatatggtc ttggagaagc tgttagaata
    21061 ctaaacaaag atttagatgc tgttatttcg aaagtagata acttaaaaaa tatattaaaa
    21121 aatgaaataa ttgataatat agaagatata aaaatcaatt ctccagaaga tggagtatgt
    21181 catatattaa atgtatcatt tagagggaca aaaggagaag ttttacttca ttacttagag
    21241 caaaaaggta tttatgtatc aactggctct gcatgttctt ctaaaaagaa aggtagttac
    21301 gtattaaatg ctataggtct tacaaatgaa gaaataaatg gaacaataag atttagttta
    21361 tctgatatga atacagaaga agaaatgtta gaagctgtga aagttttgaa agaatcaatt
    21421 tgtgacttaa ggtcaataat gaaaagaaaa taaagaatac tgtataaata gtgataataa
    21481 attagtttaa gaggtgaaat ggtgtataat atattaatag taaagtatgg agaaattgga
    21541 gtaaaaggaa agaatagata tatatttgag aataggctta taagaaatat aagaaatatg
    21601 ttaaaaccaa ttggaagatt taatgtatat aaagagtatg gaagaatata tgttgattta
    21661 gaagactatg attatgaaga agtaattgaa gaagttagaa aagtatttgg gatagtgggt
    21721 gtgtgccctg gagtaagagc taaaaaagat tatgatacat taaaagaaat agctttaaaa
    21781 atgttagaag aaaaaataga ggctggatat aaaactttta aggtagagtc aagaagagga
    21841 gataagtctt tcgggttgac ttctcaagaa atgagtatgg atataggtgg atacttatta
    21901 tcaaaggtag gagataggat taatgttgat gtaagaaatc cagaagttaa aataaagtgc
    21961 gaatacagag aatttcatac tatggtctat agtgatacta tacctggata tggtggattg
    22021 ccacttggaa cgaatggtag agctatgtct cttttatcag gaggtataga tagtccagta
    22081 gctacatgga tggttgcaaa aagaggtatg gaagtagagg cagttcactt tcatagttat
    22141 ccatttacaa gtgaaaggtc acaggaaaaa gttaaggatt tagctaaaat actggcaaaa
    22201 tactgtggta gagtaaggct tcataaagtt aatatcttag aaatacaaaa agcaattggc
    22261 gagaattgta atgaagaaga agctactatt ctttcaagaa gatttatgat gaggatagcg
    22321 cagaggcttt ctgaaaaaag acattgtgat gctttaatta caggtgaaag tattgggcag
    22381 gttgcatctc aaactataca agggcttact tgtactaatg ccgttgtaga cttaccagtg
    22441 tttagaccac ttatagcaat ggataaatca gatatagttg atatagccaa aaaaataggg
    22501 acttttgaaa catctatagt gcctgaagaa gattgttgta gtgtattttc tcctagaaag
    22561 cccgtcacta aacctagatt agagaaaata gaaaaatctg aaacagcact tgacattgaa
    22621 aaattagtac aggatgctat tgacaaaata gaggtagagg atattgagtt ttagcatacg
    22681 agttatttaa ttactaaatt gaatattgag tatattagta gttctgtaag tatattagta
    22741 gttctgtaag tatattagta gttctgttga aaggatttat gtagacaatt tattaatatg
    22801 tagtatttaa aaataagtac tataatttta tttaatggga atgattatta tgaataaaaa
    22861 gggttttaca ttaattgaat tgttggtagt tatatctata ataggaattt tagttatagt
    22921 agctgttcca gcgttattta gaaatataga aaaaagtaaa gcagttacat gtctttctaa
    22981 tagagaaaat ataaagactc aaattgttat tgcaatggct gaggaatcaa gtaaagacaa
    23041 gaatgaagtc ataaaagagg tattagaaaa caaagatggt aagtactttg aaacagaacc
    23101 aaagtgtaag tcaggtggaa tatattcagc aacgtttgat gatggttatg atggaataac
    23161 tggaatagaa agcattgcaa aagtgtatgt tacttgtaca aaacatccag acggtgttga
    23221 aatggctagg gatgtgcatc aaagtatgaa agatttgatt gcatcatttg cacaagaccc
    23281 ttctataata ccaggagcct caaagggtaa tgatgatttt agaaaatatt tattagacaa
    23341 taaatataaa aatgggtggc ctacaattcc agatgaattt aaggcaaaat atggattaag
    23401 taaggataca ctatatatac aaccatatgc atataatcct actaaatctg atgctactgt
    23461 agttgtattt gcaaataata agactggggg taattggtat acttccctag tttacgatta
    23521 tgatgaaggt agatggtata aaggtaaaaa tggtatttct gttgcaggta ggtcatggga
    23581 tgttgacaca gatagtgtta agtctgtaaa aacagagatt cattctaaag agggatgggg
    23641 tcctttaaat taatatatca tgttttatta atctggtatt gattaaacta tagaaaagag
    23701 cagtttctat agatgacgaa taccagattt tcatttatat atataattga gatatattaa
    23761 aagtgtttat atttttaagc cttcacatac aagctaaaaa taattataat aatattgtaa
    23821 agaaatttaa ttataagatt aattttctga agatttagta tttgtactaa gaaatacata
    23881 aaaaggggaa tagatatgaa taataatttt caagacaacc aaacaataaa tttaatacaa
    23941 agtcgtagaa gtatacgaaa atttacgaca gaacaaatta gtgatgagca agtaaacaca
    24001 ttattacact gtgcatttgc agcaccttca ggatgtaata aacagccttg gcacataact
    24061 gttgtgcaag accagaaact tttaaaagag attagtgatg atactttatc taggattcat
    24121 gaggtgagta atgttgagat aaataaaaac tttaagttat tttatggtgc tccaaccgtt
    24181 ttatttattt cttatgatga aagtagttca tgggctccat atgatatagg aatacttaca
    24241 gggaatataa caacagctgc acaagcacta gggcttggaa gttgtataat aggaatggtt
    24301 agaggtttat ttacacctgt tgaacaaggg gatattgaag gtcttgtgag tgtattggat
    24361 aaagaagatg taaaagaatc agagtcaata aagatgaagt ttgatacaaa taaaaaatac
    24421 agagaactac ttgatatacc agaagggtat tctgtaccat ttggtattgc tgtaggtatt
    24481 ccagatggaa atttaccaaa tgctagagag gtagtttaca aagtttccag agtttaggat
    24541 aaagtattgc aagaaccaat cagttacttg aagaattaaa tcagtaatat acaaataaat
    24601 tgatggaaga ggaagctatt taaaaagtag tttagatttt tcgttgtaaa ataattaaat
    24661 aaagaataag gtgataagta ttttatttat tatcttattc ttttttattt ttgttttgta
    24721 gaatgataaa aataaggatt aagtgtaata aaatagaaaa caagtatgtt aattttaagt
    24781 atatgcttta aaatttatcc aagttaaaaa tttaagatat cttttcagta taatggaaaa
    24841 aatgtcaaaa tattgaaata gggaaaaaat agtgatataa taagaaaaaa tagagaaaaa
    24901 agtaaccctt agttgtaaaa aagttactat ttattgaaaa tttagatatt tttctaaaaa
    24961 ttatctatgt taatagttaa atttatgaac attttttgtt tttatgttag gatatttctt
    25021 agaaatgaaa tacttcttaa tttgatttga tatgtatatt tttatagcta ttttattata
    25081 aaaaaaaaga accctttaca acaaatggaa actatggatg taataattaa aataaaaata
    25141 ttattttata tttataaaca tt
//
LOCUS       DAEMIK010000048        24938 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEMIK010000048 DAEMIK010000000
VERSION     DAEMIK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.048"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            68..1924
                     /locus_tag="KPK87_001654"
     CDS             68..1924
                     /locus_tag="KPK87_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG2537808.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYSHKREISNELSKKLEIEFSDL"
     misc_binding    2007..2247
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2313..2548
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2633..3850
                     /locus_tag="KPK87_001655"
     CDS             2633..3850
                     /locus_tag="KPK87_001655"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG2537809.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3967..4449
                     /gene="cdeM"
                     /locus_tag="KPK87_001656"
     CDS             3967..4449
                     /gene="cdeM"
                     /locus_tag="KPK87_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG2537810.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4713..5978
                     /gene="hisD"
                     /locus_tag="KPK87_001657"
     CDS             4713..5978
                     /gene="hisD"
                     /locus_tag="KPK87_001657"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG2537811.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6181..6933)
                     /locus_tag="KPK87_001658"
     CDS             complement(6181..6933)
                     /locus_tag="KPK87_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2537812.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7051..7320)
                     /locus_tag="KPK87_001659"
     CDS             complement(7051..7320)
                     /locus_tag="KPK87_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537813.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7795..8889
                     /locus_tag="KPK87_001660"
     CDS             7795..8889
                     /locus_tag="KPK87_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2537814.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8891..9841
                     /locus_tag="KPK87_001661"
     CDS             8891..9841
                     /locus_tag="KPK87_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG2537815.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     RGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVNHG"
     gene            9834..11018
                     /locus_tag="KPK87_001662"
     CDS             9834..11018
                     /locus_tag="KPK87_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537816.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DVYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11031..12527
                     /locus_tag="KPK87_001663"
     CDS             11031..12527
                     /locus_tag="KPK87_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG2537817.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12550..13500
                     /locus_tag="KPK87_001664"
     CDS             12550..13500
                     /locus_tag="KPK87_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537818.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFS
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13521..14483
                     /locus_tag="KPK87_001665"
     CDS             13521..14483
                     /locus_tag="KPK87_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2537819.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14855..15487
                     /locus_tag="KPK87_001666"
     CDS             14855..15487
                     /locus_tag="KPK87_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2537820.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15898..15990
                     /gene="kdpF"
                     /locus_tag="KPK87_001667"
     CDS             15898..15990
                     /gene="kdpF"
                     /locus_tag="KPK87_001667"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG2537821.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            16031..17713
                     /gene="kdpA"
                     /locus_tag="KPK87_001668"
     CDS             16031..17713
                     /gene="kdpA"
                     /locus_tag="KPK87_001668"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG2537822.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17738..19795
                     /gene="kdpB"
                     /locus_tag="KPK87_001669"
     CDS             17738..19795
                     /gene="kdpB"
                     /locus_tag="KPK87_001669"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG2537823.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19814..20440
                     /locus_tag="KPK87_001670"
     CDS             19814..20440
                     /locus_tag="KPK87_001670"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG2537824.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20677..21585
                     /gene="cysK"
                     /locus_tag="KPK87_001671"
     CDS             20677..21585
                     /gene="cysK"
                     /locus_tag="KPK87_001671"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG2537825.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21612..22202
                     /gene="cysE"
                     /locus_tag="KPK87_001672"
     CDS             21612..22202
                     /gene="cysE"
                     /locus_tag="KPK87_001672"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG2537826.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22362..22550
                     /locus_tag="KPK87_001673"
     CDS             22362..22550
                     /locus_tag="KPK87_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG2537827.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22604..23473)
                     /locus_tag="KPK87_001674"
     CDS             complement(22604..23473)
                     /locus_tag="KPK87_001674"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG2537828.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23670..24914)
                     /locus_tag="KPK87_001675"
     CDS             complement(23670..24914)
                     /locus_tag="KPK87_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537829.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINSKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9594 a         2676 c         4517 g         8151 t
ORIGIN      
        1 agaaaattgt catataatta attaagtagt attattataa ataacattga aaggtagtgg
       61 ttagaatatg gatacccata ataaatatgt aaattttata aaaaatatac ctgtaccttt
      121 tttatattgt agaattgtaa agagacaaga agatatagaa tatcgagtag aatatataag
      181 taagggtatg ggcaaagtat tacaattaga agaaggtatt tgtgataaaa atatattaga
      241 tgtattgcct gtatttaagt ctaaaaaata tttcaaagag ttgttctcaa atgaagtgga
      301 ttgtataaaa aggtacattc cgacacttaa aaactggata aatataaaaa aacagataat
      361 aggggattca tacataattt tgtattttgg taaaattgta tttgattata gacagataat
      421 cgattccttt gataaaaagg aaaaagtagc ttatattaaa gacgaagaag gcatatatat
      481 agattgcagt gaaaatttaa taccaatatt aaataacaac attaaaacaa cgaaagatat
      541 ctttggtaaa aatgatatag aagtatgggg agagaataca ggaaaattat ttagagatga
      601 ttatagagaa ggagtatcta gtaaaaaaag atttttacaa aatctttttg aatatgaaga
      661 aacatttttt atggttgaaa aatatttttt gtatgatgaa gacgaacttt taggtactat
      721 agggatagta gacaatataa tatatagtgg ttacagtaat agaaattata actcaaaaga
      781 tttaatgaag atgatagaac actcaattcc agagaatatg ttttataaag atgtgtatgg
      841 aaattatatt ggatttaatt caggtttttt gaatttagct tgtatgaata aagaagaatt
      901 attagggaag aatagttata aaatatcaga agaagaagct ttaatagata agatatttga
      961 aagtgataag ggtgtagttg aaaataaaaa agtagttaca tttgagttaa atataagtat
     1021 gaatgatgaa aacaagtgta ttgagattac aaaaaggccg ttttttgata gctatggaag
     1081 tgttattgga ataattggaa cagcaaggga tataagtaga agaaaaagat tagaagagga
     1141 aatggataaa actaggatgg aattttttgc aaatttatcc catgaactta gaacgcctat
     1201 aaatttaata tcatcttcat tacaagtgat agaaaaaaaa gaagcagatt taatagaatc
     1261 aaatgatact ttaaaaagaa atctaggtat aataaaacaa aatggaaata gaatattgag
     1321 gttagtaaat aatgttatag actttacaaa aatgcaatca ggatatttag attttaaacc
     1381 agaagaatct gatataattg catttataga ggagatatgt atgtctgtag ctgattttgc
     1441 aagccaaaat aatatacaac ttacatttga tacagaaata gaagaatttt caatgttatt
     1501 tgattcagaa aaattggaaa gaataatatt aaatttactt tctaatggaa ttaagtacaa
     1561 taagaaagat ggaaaaataa atatattttt atatgtgaaa gataatgtat ttaatatgaa
     1621 aatatctgat agtggtatag gtataccaaa agaaaaaata gataaaatat ttaataggtt
     1681 tgaacagatt gacaatgaac tttcttacag agttaaagga agtggtattg gactttcatt
     1741 agtgaaatct ttggttgagt tgcatgaggg aagtatatct ttaaaaagtc aacttggtat
     1801 tggaagtgaa tttatagttt cattgcctgt tagaagtaaa aataatattg aaaaatatag
     1861 tcataaaaga gaaatcagca atgaactaag taaaaaatta gaaatagagt tctcggattt
     1921 ataagtttaa atataaaaaa taaaatttta aaaaagtatt gacaattgat ttagaagggt
     1981 ttaaaataaa ttacaacata ataaataaaa aggcgatgaa taggaagagt aaatctaatt
     2041 cgattaccag agagaagagt ccgttggctg gaagactttt taaattagag ggattgaaaa
     2101 ctacctagga gctactttct gaagtcatct gcattagtag attttagtaa gaatagtcgt
     2161 tagctgcgtt aaagtgtaaa gtgagttttc atgacatatg aaaactaaat taggtggaac
     2221 cgcgggaata atactctcgt ccttatgtta taaggatgag agttttttgt tatttaaaaa
     2281 tataaacagt aatcataata attatagaaa atataaaagc aatgaatagg aagagtaaat
     2341 ttaattcgac tatcagagag gaaagttcac aggctgaaag actttctaag tttgagaaat
     2401 tgaaaactac ctaggagctg tggtctgaag ttactttaaa aagttttagt aagactaacc
     2461 gttaactgcg ttaaagtgta aagtggattt tatcaataaa tgataaaatc aaattgggtg
     2521 gaaccgcgag tatggactcg tccctttata tagggatgag tctattttta ttgcaataaa
     2581 agattgaaaa taaaaaaaaa tcggaaaatt ataaatttag gaggaaaata aaatggagaa
     2641 taaagtgaaa attggagtac taggatatgg agttgtagga agtggactta tagatataat
     2701 agataataat aaagaaaaaa gaaatataga aatagttgga attttagtta ataatttgga
     2761 aaaacataag gacaaaaaat attctaatat aataactaat aatatagatg atatatttaa
     2821 taaagatata gatatacttg ttgaagtcat gggtggttta gaaccatcat tatcttatat
     2881 aaaaaaagca ttaaacaata aaattcatgt tgttacagca aataaagatt tgttagctga
     2941 atgtggagat gagcttgcaa aattagcaag tgaaaataaa gtaagtatta aatttgaagc
     3001 ttcagttgca ggaggtatac cagtattaaa accaataata gaatcactag aaggaaataa
     3061 tatagatagt ataaatgcaa ttttaaatgg aactactaat tttatattat ctaaaatgta
     3121 tgatgagaat ctatcttatg atatggcatt aagacaagca caagaattag gttttgctga
     3181 agcaaatcca gagtcagatg tattagggta tgatgctgct agaaaactat caatattatc
     3241 aactctagct tatgacaata gagtatattg gaaagatgtg tatttagaag gaattacaga
     3301 tatagatgaa aaagatattg aatatgcaaa aaaattaaat tgtaaaataa aattgattgg
     3361 tcaaagtaag tatgaaaatg ataaagtaag tgcttttgtt agacctgttt tagtagaaaa
     3421 agataatatc cttgctagaa tagacaatga gttcaatgct gtcatagtga atggagattc
     3481 agtaggagaa gtttcttttg taggtaaagg agctggaagt ttagcaacag gaagtgcggt
     3541 ttattcagat gttatagata taatagataa tagagtttca agtatagatt cttttactaa
     3601 agataaaata caagttaata aaatagttag agaaaagtgt ggagcacttc ttagatttaa
     3661 aaaatgtaat aaagatgaaa ttctaaatat agttgagaat tgtttagtaa aattcgatat
     3721 tttaaatgat gatgatgaac ttgctattat ggtttatgct gattctgaat atgaaataaa
     3781 caacagttta tgtctaataa aagataaagg atactgcgaa aaaatgaata aaatgttgaa
     3841 aataagttaa aaaagaaaaa atataagttt atatgtaaca tattttgacg attttaatac
     3901 tatatagtat gagatgaaac aaaacatctc ataaaaaaat taaaattaaa gggagagata
     3961 tgttatatgg aaaataaaaa atgttattca gaagattggt atgaaagagg agaatctaca
     4021 gctaaatggt tccaaaatga tagagaagaa tatgaaagag aagcatatga tgaagataga
     4081 gaaagaagag gttcaaactg tggatgttca gattcaggag aaaatagacc tagaaactgt
     4141 gaaagattta gaagagaagc tgagataaga gaaagagaag caagagaagc attctgtgaa
     4201 tcttcagaga aaaagaaaga ggcattagca tatgaatgtg aagctagaaa attatgggaa
     4261 gaagcagaaa aatactggga tgaatattca aaatacaact ataaaggaat cgaatattta
     4321 gcagaagctg ctagattatt tgatgaaggt atggaatgtg aagctagaag aaatggaaat
     4381 aatggaggaa acaataataa ttgttgccat aaatgccata aatgtaattg taactgctgt
     4441 agaaaataaa aaaattaatt gacaaatcaa tagttaaaat atatcatatt atgtataata
     4501 aagagaaaat tcagaaaaaa ataatgatta gaagaaatat aagtatattt attagacaaa
     4561 tagtgctcag ttagtgaggt gagtacaata tgattaaact cccatcctct agcttttgat
     4621 agagggtggg agtttttatt tatttatata tcttaaatga ggataatatt gtaaaataaa
     4681 tgagtatgag attgtaaaat taggaggtaa aaatgaaaag tattaaaaca tcaagtatga
     4741 aagaaaaggt agatgaaaag atattaagag agcaagtaag ttctataata gaagatataa
     4801 gaaataataa ggatgttgca cttaaaaaat ataatgagaa gtttgataga aatacaagag
     4861 atgaatttag aattactaag gaagaaataa aggaagctta taagcatgta gatgacgaat
     4921 ttataaataa cttaaagatt gctgccaaaa atataagaga atttgctaaa gctcaaaaac
     4981 taagttttga aaaccctttt gaaaaagaaa tttatccagg tgtaatctta ggtcaaacta
     5041 atattccaat agagtcatgt cttgcatatg ttccaggagg tcagtatcca ttgttttcaa
     5101 cagctcttat gcttataata cctgcaaaag tagcaggagt aaaaagaata gtggcatgtt
     5161 caccaactat gaaaaatact gaaaagataa atcctaagac attggtagct atggatatag
     5221 caggtgctga tgaaatatat gcaactggag gagttcaagc tatagcagca tttacatatg
     5281 gaacagaaaa gattaatcca gttgacataa ttgtaggacc aggaaataag tttgtaactg
     5341 aagctaaaag acaatgttat ggacaagtag ggattgattt tgtcgctggg ccaagtgaag
     5401 ttcttataat agcggatgaa acttcaaatc cagtttatat tgcagctgat ttattagcac
     5461 aatgtgagca tgatttaaat gcaagaggaa tattattgac aaatagcttg gaagtagcac
     5521 aaaaagttga aaaaaatata gaaagtatgc taaaagattt gccaacaaag gacatagcat
     5581 atagttcatg ggaaaataat ggagagataa tattggttga tgatatggaa gaagccatta
     5641 aaataagtaa tttttatgca ccagaacatt tagaagttgc agtaaatgag tataatgata
     5701 tttgtgatag attaactaac tatggttctt tatttattgg aaatctttct gctgaggtgt
     5761 ttggtgacta tgtttcaggt accaatcata cattaccaac acttaaagca tcaagatata
     5821 ctggtggtgt ttgggttgga acttttatca aaacttgtac aaagcagata tttaatgaaa
     5881 aagctataca atcattagca cctgtagctg aaaagctggc aaaagaagag ggattatatg
     5941 cacatgcaaa ggctgctgag gttaggttta aaaagtagta aaaaatttta ttttggcagt
     6001 cgagacttag tattagactg agataaggaa aacgctgaga acctgaataa atactatatt
     6061 ttgtgggtct cggcgcttct tttatatctg tatataatag aataatacta tctgtgtgtt
     6121 taaatactaa cttaatattt cttttctaaa taatgtttat gaaatagtta atatttttag
     6181 ttaatacttg aaaaaaactt ttgctaactg tgcaaattca tctgcatttg tcaatatagc
     6241 agggtgtcct ccctgactaa aaatgtaaat acttgctttt gaaataaaag ttgccatttg
     6301 cttgtactct tcttcaagat tatgacgaca catttcgtct tccttacttc ctgtaaataa
     6361 aattggcatt tctaatttac acaattcttt atgaaacaaa gggcgtttct cgtttgcaca
     6421 ttgcaataga gcttttgtat caagattaac aacattctcc caatcttttc catgacacca
     6481 ttcatagaat tttcgtgctt gaatatcttg ctttgatttt tcccttccag tgattagatt
     6541 atctatgaag ttatcattaa gagtacgtcc atcaaagcta tctgctataa ttgcttcaac
     6601 taaatcggga cgttctaatc cagtattaac agctgcccat gctcctccac ttgttccaat
     6661 taagccaact ttaccacaat tcaaatgttc aatcagtgct attgtctgta aggcttcatc
     6721 atgccatatg tcaggagaaa attgagaaac tctatccgac tgtccattac ctaaaaaatc
     6781 aattaagata catctaaaat tttctgcata taatggcaca agtgctttaa acatattaga
     6841 agaaccagta ttaccatgca ggaaaattat cggtttacct tgtccatatt ctttataaaa
     6901 acaagaatta ccttgatact gaaaatatga catatatttt cctccttttt atttgttaag
     6961 ttaattataa caatataggt atgaatgaaa aacctcatgt tctttcttag aataaaaatg
     7021 tgccacgcat aaaacgtgac acatttctaa ttataaattt tgctgtttaa gtattctttg
     7081 tatgcttttc tctgtcaaaa agtatttctc tgataactgc ttgatactca ttccattttg
     7141 gaaatttata cagatttgag aattacgaga agctaagact tgtttggtgt ttgtgttttc
     7201 accccaatgc tttttgtgct cttgcttttt gggaatataa atcactttac catctatata
     7261 ttcttgcact tgctcaatca gttctttagg cagtaagtca atcgaatttt gatagctcat
     7321 tttgcacctc ctaaataatt attctttagg attagcaaag gctactgcca ctcacaatta
     7381 aaaataaaat tttttattat gtacaatagc ctttgctatg cacaagttcc aattcccata
     7441 aactaaatac tcctttctgg tttgaaaaat tcatctgaac attgatttcg cttttaagat
     7501 agagttcaat gacaaattta catttttatt atatatattc agatagataa ttgcaactgt
     7561 ctttgtatga ggatatttta ttatagtcaa tactataata tgtatttata tcatcaaact
     7621 aatatgaaca gttgaaagga atatgaacaa taaaacttag tcaggaagaa taattatgaa
     7681 gcagacaatt gagaaatttg attttaaagt tttattgaaa atttattgac aaatttaact
     7741 tgtaaatatt atactaaact taataaataa agtttataaa gtaaggtgtt gtctatgaaa
     7801 aatatagttg gaaaaccgca aacgataaaa aaagtaaatg aagatttaat aaaaaacatt
     7861 ataaaaaatg aaggtcctat aacaaaacca gaaatttcaa gcattactga attgagttta
     7921 gctacagtta ataagattgt agagcaatta gctttgaaaa atgaagttaa agtaagtgga
     7981 ctaagtgaat ctactggagg aagaagagct caattatttg aaataaatgc aaacttagaa
     8041 cacataatag ctttatatta ttatagaaat tgttgtatag gggtagttgc caatttatta
     8101 ggtgaaataa tttatcaaga agaatttaat ataagaatgg atacgtatga aaacgttaac
     8161 caagatactt tttttgttat agatactcta ataaaaaagg aaaaaggaaa caatataagg
     8221 gctattggaa ttggtgtccc aggagttgta aaaaaaggtg tagttaaaaa tataccaact
     8281 ataaaaagtt gggaaggtat aaatttagaa aatttaataa agagtaaata caatactaaa
     8341 gtgtttatag aaaatgatac aaatattact actattggga tttatcaaaa acagtataaa
     8401 gacaaatata aaaatatggc tcttatgtat ttagaagatg gtattggttc aggtatagtt
     8461 ataaatggtg aattgtattt aggttctaca aattttgctg gagaattaag ttatctaaat
     8521 atagaatttg gtaagtataa ctctaacaac ttaaatcttg aagattatat tatgagttta
     8581 agaaaaagat atttagaaag taaagataat aaatttaaga aagaaatttt atctataata
     8641 tcaaacttta taaggactct tgtatgtgtc ctaaatccac aagctatgat tatacagtgt
     8701 agtatattaa ccaagagtga catggagttt atagaaaaag atgtaagcaa taatgttgga
     8761 gaagaaaatt ttcctaaatt aataaaagta gactcattga gagaaggaag tataaatgga
     8821 gtaattagta tgtgtttaaa agaaactgac tatcaatatt ccttatctaa taaaaaagga
     8881 ggatattaat gtgaaatatt tggtttcaat agatggaggg ggaacaaaaa ctaaattttg
     8941 tgtaagtgac ttagatggaa acatacttaa agaacatacc actggttcaa ctaattataa
     9001 gtcagttgga attaaaaaaa catatgaaaa tattaataat ggatttaaaa aaatattaaa
     9061 agatttatat atcgattatg acgatattga gtacactgta tttggtatat ctggttgtga
     9121 ttctcctaat gattataaga ttataatgga tgaaatatta aaaattggga ttaacaaaga
     9181 aaaaatatat ctggcaaatg atgcagtttt agctttttat gcacaagctg attcccctgg
     9241 tttagtaatt gtggctggga caggttcaat tatactggga attaaagaag atggagaaat
     9301 atatagagtt ggtggatggg ggtataattt tagtgatttg ggttctggat atgatatagg
     9361 gagaaaatta ttaaagaaag tattgcttta ttgtgatgaa tgtcatgagt atagtgattt
     9421 atttagttgt gtcttggact tctttggagc aaatagtttt gaacaacttg catatatgat
     9481 tactgatata aatgataatg ttgaaatagc aaacttagct tcattagtga ttgattgtgc
     9541 cgaacgaggt gataaattgg ctattgaaat attaagagaa tcatcaactg aactatctaa
     9601 attagctcaa gtaatattga gaaaaatatc taatccaatg aaaatggata aaagcgaaat
     9661 aaatatagtt ttgtctggtg gtacattaaa taaaactgta tatgctcaaa tgttaatgaa
     9721 taatttaaaa acagaaaata cagataaaaa tttaaaattt atatctcaag aaaatcagcc
     9781 agtatatggg gggataagac ttgctatggc attagctaat aggagggtca atcatggata
     9841 gaaaaaaggt aatagtacaa atttttactg gaggttttaa ggctaaagaa ataaagttta
     9901 atgatataaa atgtaaacta ctagaattat tggactgtat tgatatagaa aaggttatca
     9961 tgggatggag tgtagatgaa gaattatatg tagaaactaa aaaactatta aataaatata
    10021 atgttgaact atatctttgg ttgccatgtt tttctgaaat aggattactt gaaaaaagta
    10081 atttactaat tgattatatg gggaaggaag tcaagggcta tgctttacaa gaggatgaaa
    10141 attttgagtt ttattgccca aataataagg aaaatataaa aaatataaag aaaatatata
    10201 gtacttattt tagtgatatt aaatttgatg gaattttttt agataaaata aggtatggtt
    10261 ctttttcgaa taaactaaat ggtgttttta attgtttttg taaagattgt ttaaatatat
    10321 atgaaaaaaa taatattgat aaagagtcgt taatgagaga aatgaataat gtgtctaagg
    10381 gagttataga taatgaagat actccatttg gtataacaga atacattaat ggaaaataca
    10441 aatttaaaaa taaaatatgg gaagattttt ttgaaacaaa aaatcaaaat atatacaagg
    10501 cattattaga gataagtagt tattttaaaa gtaagggatt gaaaataggt atagatactt
    10561 tttctccttt tatttcttac tttacaggtc aagatttaga aaaattacaa gaagtagcag
    10621 attttataaa accaatgatg tatagaacta caaaagctcc agctggttta ccatttgaat
    10681 ttgaaaggtt tattgatgaa tccatacata agaataaaga gattggaaaa agaaatttct
    10741 tagatatttt aggaattaag gagtatgaag gagatgtata ccctatagaa tttgtactag
    10801 aggaaatcga ttttatgaca agaaatctta ataaagatgt atatgtagga gttgaaataa
    10861 ataaaaagga aattatatct cctgttacac ctaagtacat aagtgaaaat atttcaaata
    10921 taaataagac aacagtaaaa ggatatgttt tatcttggga tttgttgtca gcaccaaaag
    10981 aaaatattga tgaaattata aaatggtttc ggaattagtg aggtgataaa gtgagtaaga
    11041 tattggaaat gcaacaaata tataaaagtt ttggaaaggt agatgtttta aaagggataa
    11101 attttgagtt agaaaaggga gaagtaagag ctcttttggg agctaatgga gcaggaaaat
    11161 ctacacttat taaaatttta ggaggagttc attctcaaac taaaggtaaa gtaattttga
    11221 ctggtaatga aataatgttt aaaaatccac atcattctaa agaaaatggt attagtataa
    11281 tttatcaaga attaagcttg ataccaacgc ttacagtact tgaaaatatt tttcttggta
    11341 gagaaatttt aaaaggaagt gtgtttttaa acaagaaaga catgcttgag gaatatatga
    11401 aaatttgtga ggattttgat tttaatatag acccacatat agtagtttct aatttaagtg
    11461 tagcaaacca acaaatggtt gagataatga aagctgtttc atgtaattct gaaattatca
    11521 taatggatga acctacaaca tcacttacta ataatgaaaa agatgggcta tttaaaataa
    11581 taagtagact aaagtctaaa ggtaaaacaa tagtctatat ttctcacata ttagaggaag
    11641 tttttaaagt atgtgatagt gcaagtataa tgaaaaatgg aattatgatt ggaacttatg
    11701 atgtagagga tttaactaaa ttaaagataa ctcaacttat gacaggaaat gataatcaca
    11761 aacttattga gaagaaggat tatacatttg caaaatatga agagccacca gtactagaag
    11821 taaagaatat aagctggtct aacaaaataa aaaatgtttc atttaaagtt tatagaggcg
    11881 aagttgtagg tcttgcaggt cttgtaggtt caagaagaac tgaaattata aatttgatat
    11941 ttggtataga taataaagat agtggagata tttttatcaa tggtaaaaaa gtagacataa
    12001 cttctcctaa ggttgctata aaaaacaaaa taggattgat tcctgaagat agaaaaaatc
    12061 taggattaat tttagaacaa gctatataca caaactcatc atcagtacaa attgataaat
    12121 tcaagaaatt cttgtttata gatagaaata aagaaattga gttttctaaa aaaggtgtta
    12181 aggaattagg aataaaagtt agtgatgtaa agcaagaagt aaaagagctt agtggtggaa
    12241 atcaacaaaa agtagtagtt tctaagtggt tagagaaaga tttagattta ataatatatg
    12301 atgagccgac aaagggaata gatattgctg caaaagaaga tattttcaat acaatcaaag
    12361 aattttcaag taaaggaata ggagtaatat ttatatcttc tgatttagaa gaagtaataa
    12421 gagtaagtga tagaatttta gtaactagag aaggttctat tgtagatgat ttaaaaaatg
    12481 agaatataca ggttcaagat ataatgaaca gtatatttaa tgtataaaaa atgaaaatgg
    12541 aggactgaaa tgaagaaagc ttttgagaag ttaaatttaa gagattatgg agtattatta
    12601 ggttttttag ctttatgtat agctataagt attgcaactc catcattttt aggtaaacaa
    12661 aatatactta atcttttacg acaatcttct ataattggaa taatttcagc aggtatgact
    12721 tttgttataa tatctggaaa ctttgatata tcagttggag cagtagcagc tcttgcaggt
    12781 gctataacaa tgaaatttgc gactggtgga actaatttgt ttgcatccat gctcttaggt
    12841 gtagcgattt gtgcagtaat aggattaatc aatggtatag tagttgcaaa gataaatgta
    12901 ccatcattaa tagccactat ggctatggtc actattgtaa gaggattgct gttgatgctt
    12961 acaggaggat atccaattac tgaaaatata ccaatacttg attacattgg aaatggatat
    13021 ttgtttagta taccaatacc agtagtaatt ttctttatag tggtgttagc ttcgtttatt
    13081 gtacttaata aaactaagtt tggtagatat gtttattctg taggagggaa tcaagaagca
    13141 agtaaactta atggtataaa tgtagattct cacaaagtga aagtgtttat tattaatgct
    13201 gtacttgcag gaatagctgg aatcgtacta acaggaagac ttgggactgc aactgcgatt
    13261 gcaggtgaag gatatgatat ggatgcaatt gcatctgtag taataggagg tacatcagtg
    13321 gcaggaggtt ctggttctgt acttaaaact gttataggag tattacttat gagtgttata
    13381 aacaacagtt ttaatcttct tggaatagat gtatatttcc agtacatatt taaaggatta
    13441 ataattttag cagcagttgg atttgattct tatagcaaga aaaaacttgc ttcaagataa
    13501 aaaataaggg ggattttttt atgaaaaaat taatgaaaca tttagcattg ttgttatcac
    13561 ttgttatgat atttggttta gttggttgtt caaatggtgg tgacaaagaa aaaaatggag
    13621 aaaagaaaat agcagtatta ctaccaggtt caacaggtta ctttgtagca actaaacaag
    13681 gtatagatgc aaaatctaaa gaattaggtg taagcgttga atatgcagat gctcaatggg
    13741 atgctagtaa acaattatca caagctgaag attttatggc taaaggggta gacatgattg
    13801 cattatgtgg agttgattct gctgtaagtg agagaatagt taaagctgct aatgattcag
    13861 acgttcctat agtagcattt acaaattcta taggaagtaa cccaactgga gaatttaaag
    13921 gtttagttac atatataggg caaaatgagg aagaaacagg agcattaact ggaaaaattg
    13981 ctaaaaactt attaggagaa actggtggga aagctgtatt aatagaagga gtaccaggaa
    14041 ctactccaca agtattaaga aaaaaaggtc ttgaaaaaga attaaaagat agtaatatag
    14101 aaatagttta taatcaaact tcaaattggg agaaagagca agctttaaag gtaacagaag
    14161 atttaattca aaagaaaact gattttaatg ttataatatg tcaagatgat aattctgcta
    14221 caggtgcagg tcaagcttta aaagatgctg gtcttaagga taaagttaaa gtcataggtt
    14281 tgggtggaag taaagatgga ttaaaagcta taactgatgg gttaatagat ggtacaactt
    14341 atatgtctgc tgtagaagaa ggtggattag tcatagaaaa ggcatctaaa ttccttaaag
    14401 gtgaaaaaat agaacctgtt acacaaatta aacaagttga agttaacaaa gataatattt
    14461 cagagttcaa gggtgaatgg taaattgtca tagataataa ataagatttt tagagttact
    14521 ttagaaacta tataaagtaa ctcttttttt aatttaattt agtgataaat tattgaatag
    14581 tatttctaaa caaataaaaa attaatataa acaaagactt aattatacta aattataagt
    14641 aaaactaata taaacttatg gaaaaaagga aaacgtctaa agcattggaa ttactatagt
    14701 gtaggtgtgg tattaaaaaa acataaaaaa acataaaaaa attgttgaaa aaaatataga
    14761 aattttgtat aattaaagat ttgaaaagtg cggtatacaa agccaattct tttgtatata
    14821 atatatataa attcagaaaa aggaagggat atgtatggcc aggcggttat ttttagaaag
    14881 gattggactt atatttttag gttcagcaat attagcattt ggagtttata atttttatta
    14941 tttaaacaac ataacagaag gtggagtgtt aggtatttta ctactgttaa aaaatttatt
    15001 caatatacaa ccagcaatag caaatgttgt aatagatggt ttactacttt tagtaggata
    15061 taaatttttc ggtaaaaaat tccttatata ttcaatagta gcttctataa ctttttcagt
    15121 gttatatgac ttatttgaag ctataggacc tctagtacca caatcacaaa atatgctttt
    15181 atcaactatt ttggcaggtg taactgttgg aactggtgta ggtatagtag taaaagcagg
    15241 ttgtgcatca ggaggagacg atgctttagc attagtaatt tctaagacta catctttgaa
    15301 catagggcaa gtttatttag caacagatgt tattgtattg ttgttatcat tattttatct
    15361 atcagcgttt gatatctttt attctttgat tgctgttaca attagtggca aggtaattga
    15421 ttttatttat tatcatggta aaagtttgga tatggatata agtaatgata tagttccaga
    15481 atgttaatat aaattaaata actttagatt ttattagggg tggcagattt gccacctttt
    15541 ttgtgtctaa atctttatag aaagtataat cccatctagg tttttgcaaa tctcaaatat
    15601 agaattatgt tggaatgact gggaatacga ttgccagatg tgcattttaa aaaccaaatt
    15661 ttatatttat gaaatactaa tcattaatgg atgtttctta atcctatctt tatacctaaa
    15721 atgataagat aaatgaagta gcaatacaaa gctaaaattt gtaaaagaga ttaaattaaa
    15781 tggaggagct aaaatgttgg atatagttat gatagcattg ttattcatag gatttgtttc
    15841 catgaagtta cttgctgatt ggtgtgggaa acaaactgaa aaaaaataat gtaatgtatg
    15901 gagggattac ctatgtggtt tctggcagtt gtaattataa tgttaataat atatttggta
    15961 tatgcgctta ttaatcctga aaaattttaa acagtataag gctaataagt aagttgaatt
    16021 ggggggaaca atttttatgg tgcagatagt aatagtgtta gcgattttta tgattttagt
    16081 aatacctatg ggcaagtatt tatatcatat agctactaat caaaaaacat ttggagatag
    16141 actatttgat aaagtggaca attttatata taaggtatgt agtatagata agaaaaaaga
    16201 aatgaactgg aaacagtatg cattagcttt attatttaca aatgcagtta tggtatttat
    16261 aggatatatc atacttagaa ctcaaagtat gcatattttt aatccaagtg gaattaaaag
    16321 tatggaacaa ggactatcat ttaatacaat aataagtttt atgacaaata caaatcttca
    16381 acattattca ggtgaatcag gactttcata ctttagtcag atgacggtga taatatatat
    16441 gatgtttaca tcagctgcaa caggatatgc ggctgctatg gcatttgtaa gaggtctagt
    16501 cggtaaaaag aaaacattgg gaaattttta tgtagattta attaggataa caacaagagt
    16561 acttttacca ggagctttaa ttattgggct tatattagtt acacaaggag taccacaaac
    16621 ttttgctggg acagaaacag tgactactat agaaggaaag cttcaagata tagctagagg
    16681 tcctgtggca gctcttgaat caataaaaca tttaggaaca aatggtggtg gattctttgg
    16741 ttcaaattca tcacacccat ttgaaaatcc aacaataata tcaaatatag tagaaatatt
    16801 atctatgatg atattaccag gagcttgtgt tgttgcattt ggtcacatga taaagaataa
    16861 aaaacaaggt tgggttgtgt ttggcgcaat gtcaatcata tttttaatag gtctagtagt
    16921 gtgctttaaa gcagaaagtg caggaaaccc tattttatca caattagggc ttaatcaaag
    16981 tatgggaagt atggaaggta aggaagttag atttggtata gcacaatcat cattattcac
    17041 aacagtgact acttccttta cgacaggtac agttaataat atgcatgata cactgactcc
    17101 tttaggagga ttagtaccac ttttaaatat gatgctaaat gtagtatttg gtggtaaagg
    17161 cgtaggtctg atgaatatgt tgatgtacgc tataatagca gtattcttat gtggattaat
    17221 ggtaggaaga acaccagagt ttttaactaa gaaaatcgaa ggaaaagaaa tgaagcttat
    17281 tgctttactt attatattac atccactttt aatactaatg ttctctggtc tttcagtagc
    17341 aataccagcg gggcttgaag gaatttcaaa tcctggattc catggattat cacaggtatt
    17401 gtatgagttt gcatcttcag cagctaataa tggttcagga tttgaaggtc taggagataa
    17461 cactatgttt tggaatataa caacaggtat agttatgttc tttggaagat atgtgtctat
    17521 tatagtacta ctagcaatat caagtttgtt agcatctaaa aaagctgtaa atgaaagtat
    17581 aggaacactt agaacggata attttacatt tacaatagtt ttagtacttg ttgttttaat
    17641 tgttggtgct ttaacatttt tcccagcctt agcgcttgga cctatttcgg agcatctagt
    17701 gttgtggcac taaaaaattg ggagggagta aagttttatg gaaactaaaa aaacaaagtt
    17761 tattacaaaa gatattttca aaacttccat tatagaagct tttaaaaaat taaatcctaa
    17821 atatatgatg aaaaatccag ttatgtttgt tgtagaaatt ggatttatag taacattgat
    17881 acttacaata gtacctagtt tgtttggtga tgaaggcaat aacttaagag cttataatgg
    17941 gattgtaaca ataatcttat ttataacagt cctttttgca aattttgctg aagctgtagc
    18001 tgaaggaaga ggtaaagctc aagcagatac acttaagaaa actagaaaag atacagtagc
    18061 aagggttata gatacgttag gaaatgaaaa aatgataagt gcaagtgagc taaaacttgg
    18121 tgatattgtt ttagtaaatg ctggagaagt tatcccaaat gatggagaag ttatagaggg
    18181 tatagcttct gtggatgagt cagcaataac tggagaatct gcacctgtca tgagagaatc
    18241 aggtggagat ttcgcatctg ttacaggtgg tactacagtt gtaagtgact ggttaaagat
    18301 aagaataaca acaaaaccag gagaatcgtt cttagataaa atgatagctc ttgtagaagg
    18361 tgcttcaaga caaaaaacgc caaatgagat tgctttaaat actttgttag tagggttaac
    18421 acttatattt atggttgttt taatatctct ttatccaatg gcaaactatg caggtgtaaa
    18481 gattccaatt tctactatga tagctctttt ggtatgttta ataccaacta ctataggtgg
    18541 attgttgtca gctattggta ttgcaggtat ggatagagta actagattta atgtaattgc
    18601 aatgtcagga aaagcagtag aagcttgtgg agacgttgat actatgattt tggataagac
    18661 aggtacgatt acttatggga atagacttgc atcagaattt atagcagttg gaaatgcaga
    18721 ttcaaaagat ttaataaatt attctgttat gtgttcatta aaagatgata caccagaagg
    18781 aaaatctata gttgatttag gtatgaaatt aggttcaaca ggtaagacaa aagaggcaga
    18841 ggaagctgag tttgtagaat tttctgcaca aactagaatg agtggtatta acttagctga
    18901 tggtagagct ataagaaaag gtgcttatga tagcataata aaaagagtaa aagaatctgg
    18961 tggtacagtg ccagaagatt tagaagaaaa tgtaaatcga gtagctaaac taggaggaac
    19021 acctttagta gtgtgtgtag ataatgagat atatggaatt atctacttaa aagatacagt
    19081 aaaatcaggg cttgtagaaa gatttgctag acttcgagag ataggaataa agacaataat
    19141 gtgtacaggg gataatccct tgacagcagc tacaattgca agagaagctg gggtagatgg
    19201 atttattgca gaatgtaaac cagaagataa aatagatgca ataaaaaaag aacaattaga
    19261 gggtaaaata gttgcaatga caggtgatgg aacaaatgat gctcctgcac ttgctcaggc
    19321 agatgtaggt cttgcaatga atagtggtac aacatctgca aaagaagcag caaatatggt
    19381 ggatttagat tcagacccaa ctaagatatt ggaagttgtt gaaataggaa aacagctttt
    19441 aataactcgt ggggctttga caacatttag tatagcaaat gatgttgcaa aatattttgc
    19501 gattataccg gctatattta ctcttgcaat acctcaaatg gagatattaa atgtaatgaa
    19561 actttctacg tcatttagtg cgatattatc agctttaata tttaatgcta taattatccc
    19621 atgtttaata cctcttgcaa tgaagggagt aaaatataga cctatgaaat cacaagctat
    19681 gttaatgaga aatatgctta tatttggtct tggtggagtt atagtaccat ttttaggaat
    19741 aaaattaatt gatttattga ttacaccttt agtaatggtt cttaatttag gttaataaat
    19801 ggaggatata aaaatgaaaa cattaaagcc agcattactt gtaagtattg tattgttagt
    19861 tgtttgtgga cttgtatatc cacttgtttt gacaggtgta agccaagttg catttaaaga
    19921 taaagctaat ggaagtatga tagaagtaaa tggtgtaaaa gtaggttcag aacttattgg
    19981 tcaaagcttt acagatgcga gattctttaa gggaagagta tcatcagtaa actataatac
    20041 ttatacaaaa gaagatttag taccagataa agatggaaat acatcttatg gaggtgtatc
    20101 atcagggtct tttaattatg gagctacaaa tccagaactt catgatagag tgcaaaaaga
    20161 tatagaaaag tttttaaagg ataatccaac tgtcaaaaga gaggatatac caacagattt
    20221 acttacagca tcaggttcag gacttgaccc taatataagt ccagcctctg caaaaattca
    20281 gataccagct atagcaaaag cgtctggaat atcagaaagt aaattacaaa aaattgtaga
    20341 tgataatact tccaaaaaac tttttggagt tttaggtgaa gatagagtta atgtactaaa
    20401 ggttaatgta gaggtcgcaa aaatattagg tcttatatag tattttgtag aattttagta
    20461 acaaaatatt acgcaagatt taatttaaag gttaaatttt aaagagtgaa aatcaataat
    20521 gattttcact tttttatttg aatattacta atatttggaa taaagtgatt gtaagtataa
    20581 aatccaaaaa aaggttgcat tatcaaaatg ataatactat aatatattta tcgttatcaa
    20641 tttgataata gatttggaca tataggagga aatagtatgt tatataataa cgcattagag
    20701 ttgataggaa acacacctgt agtaaggctg aataatttag gctgtactaa tgggtgttct
    20761 aacatatatg taaaattaga aaaagtaaat ccagcaggaa gtataaaaga tagagctgtt
    20821 tatgaaatgt tagagggctt agagcaaaga ggagaattaa aaaagggaga tgtactagtt
    20881 gaagcaacta gtggaaatac tggtattgca ttatctatga taggaaaatt aaaaggatat
    20941 gaagtagtaa tcgtaatgcc cgaaacaatg agcgttgaga gaagagattt aatgaaggct
    21001 tatggagcaa atttggtgct tacagatggt aaagctggaa tggcaggttc catagaaaaa
    21061 gcaaatgaac ttttaaaaga aaatccaaac tataaaagcc taaaacaatt tgaaaataaa
    21121 gataatccaa atgcacatta taaaacaaca gcagttgaaa ttatgaatga tgtcaaagat
    21181 ttagatatat tcgtatgtgg agttggaaca ggtggtactt taataggaac tgcaagatat
    21241 ttaaaagaac aaaatccaaa tataagagta attgcacttg aaccaaaaaa atctccagct
    21301 atatcagaaa acaagactgg acctcataaa atacaaggaa taggtgctaa ctttgttcct
    21361 gaaaattatg attctagtgt agttgatgaa gtaatattag tagatgatga agatgcattt
    21421 gatacagtta aattacttgc agcaaaagag ggaattttag tagggatatc ttctggagca
    21481 aatatatttg gtgctataga gatggcaaaa aagtatccag ataaaaagat agttacagta
    21541 gctccagatg gagttgataa gtatatgtct atgggaatat tttaataatt ttatttgagg
    21601 gagtgaatac tttgtttaag aaaataaata aagatattga atatataatg aagaatgatc
    21661 cagcagctag gtctaagata gaagtttttt tactttatcc atcagttcat gcaatgataa
    21721 tgcataggat ggctcatgct ctttataaga agaagaagct ttttacagca agattaatat
    21781 cccagatatc cagatttatg actggcatag aaatacatcc tggtgctaaa atgggggaag
    21841 gtatattgat agaccatgga atgggtgttg taattggaga gacagcggaa gtaggtaata
    21901 gggtgacaat atatcaagga gctactttag gagctacagg aaaagatact ggaaagagac
    21961 atccaactgt tggtgatgat gtattaattg gtgctggaac aaaaatacta ggacctttaa
    22021 atataggttc aaattcaaaa attggtgcaa attctgttgt cgttaaggat gtacctaatg
    22081 gtgcaacagt tgttggtata ccagcaaaaa tagtaaaaat aagaaaccta gaacctgtaa
    22141 aaaagaataa aaaagaagtg tcctatgagt atgatgaatt agataatgta tattatatat
    22201 aggatttata taactataaa ataaacttta taaaaattat tttaaaaggt atttttttca
    22261 aatttgagga cataataaaa aagaactgaa tgttacagtt ttggttcatc aatctttgat
    22321 aaacaagatt ttaaatttgt agcgtctagg aggattttat tatgaaagca aatgttaatc
    22381 aagatacttg tataggttgt ggattatgtc catcaatatg tccagaagta tttgatatga
    22441 aggatgatgg gaaatcacat gttatagttg atgaagtacc aagtgatgct gaagaatcag
    22501 cagcagaagc tagagaaagc tgtccagttg atgcaataga tgtgcattaa taaaataaat
    22561 gcctataaaa attgaatttt caatttttat aggcatttgt cttttattca gttagtattt
    22621 caacacctgt ttctgtaact aaaaccatac attcccattg agcagataat ttaccatctt
    22681 ttgtataaat agtccaatta ttttcttcat caacaaaaat ttcgtgagtt ccttcattta
    22741 ccataggttc tattgtgaat atcatcccag gaaccaatag catctcagtc ccttttttgg
    22801 atacataact tacaaaaggg tcttcatgaa agcttaatcc aactccatga ccacctattt
    22861 ctctaacaac agagtatcca tttttctttg cataagagtt gatagcatca cctatatctc
    22921 ctaaaaatcc ccatggtttg gcaacttcaa gacctttttg gacacattcc tttgtaactt
    22981 gaactaattt ttttgcattt tcattaacat tacctatcat aaacattctt gaagcatctg
    23041 aatagtatcc attaaaaata gtagaaacat ctacatttat aatatctcca tcttttaaga
    23101 taatgtcctt acttggaata ccatgacaaa cttcattatt tactgatgta catacacttt
    23161 taggaaatcc attatagtct agtggagcag ggatagctcc attttgtagt gtaaagtcat
    23221 gaaccaactt atttatatct tctgtagaca ttccaatttc gattttatct gaaatataat
    23281 ccaaaatttt tgtgtttatt acagcacttt tttttatttc ctcaatttga ctttcatttt
    23341 ttataatatc atgagttggt acaatatgac ctaacttagc aaatgaatct attttattat
    23401 ccatatccat atgacatttt ttatattttt tagtacttcc acaccaacag ctatcatttc
    23461 tatttaactt cataactttt catccttctt ttattattta tttaaattat gggttacata
    23521 aactatttta aaacataacc attataaata ttataacata aatactaaga aatcctttat
    23581 agatagtgat ttattcaaag tataaattat tatatggtta ttgttgatga aaagaggata
    23641 gttaaatgta ttattggata ttagcgcact taatagttta gttcaagttt ttcaagaaaa
    23701 tcatatgtta gaggacaatt ttcctttaaa gtattttttg aatcttcatc aagataataa
    23761 tgtacaaatg attctgcaaa aaattcttct ataaacttgc ttatatatgc atgtgcaact
    23821 tgcttagggt cataaagctt atatctttcc tgagcaaata cttccataaa ttcttcagaa
    23881 tttgattttt tatttaaaac atttttatct atagcatgtg cagtttcatg taattctaaa
    23941 ttttttgatg aatgtacatt tgcatagcta ggattactat aacctatttt agccactata
    24001 gaattatttc tatatatacc aggtacatct ttccaagtct tgcctgatct tacccaacat
    24061 ggaggtaatt gatattttaa atcactaaat tctggttcat cagttagatt tgaatttatt
    24121 aattttattt ttatatcctt ttctttaagt gtctgtaaat atttactatt tatttttgca
    24181 agtctgtttt tcatattttc aacttcatct ttatcataat tcaagtcatc aggaagtata
    24241 agcatttgtt caattatatc tatactactg tcttttatat tttcagattc ttcatcaaat
    24301 ttatattgaa gagtattatt ttcatagata gttatatttg tattattttc tatagaataa
    24361 gagtgaatat cctctaaatc taaaatattc atttgttgta aaatatgttt tgtttcgcca
    24421 ctccatacta ctaaagaaag tgaattaaaa ttaattagtg gattttcttt tgcttttata
    24481 tcattgctta tatctacatc aaatacatat tcaattgagt cttctatgtt aagactatct
    24541 gtattctttt taatatataa atacgcttgt tttaagtcta tataaatctc atctaaagat
    24601 tcgtcataaa attctgataa attattagta aagagttctt cacttaaaag ttcattttta
    24661 tcagttatac tttcaattaa aatattaatt tcttttatca tatcgtaaat acttattttt
    24721 ctatttgctt tttcactaga tacattgcat ggcgcaagag caatgcaata aaaatctttt
    24781 tcttttttat aaaaggcttt aagtgatttt gataaaagtg taaagtctac acctatctgt
    24841 aagttgacct taagtgagtc attttgtaat aattcagtat tatttctttt agctgtattt
    24901 aaattgtttt ccataatcat tgcccccttt ttattagg
//
LOCUS       DAEMIK010000049        24804 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEMIK010000049 DAEMIK010000000
VERSION     DAEMIK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.049"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            583..1605
                     /locus_tag="KPK87_001676"
     CDS             583..1605
                     /locus_tag="KPK87_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2537830.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            1901..2821
                     /gene="pyrB"
                     /locus_tag="KPK87_001677"
     CDS             1901..2821
                     /gene="pyrB"
                     /locus_tag="KPK87_001677"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG2537831.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILDLAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            2860..3552
                     /locus_tag="KPK87_001678"
     CDS             2860..3552
                     /locus_tag="KPK87_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG2537832.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3545..4447
                     /locus_tag="KPK87_001679"
     CDS             3545..4447
                     /locus_tag="KPK87_001679"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG2537833.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4532..5116
                     /locus_tag="KPK87_001680"
     CDS             4532..5116
                     /locus_tag="KPK87_001680"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG2537834.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5331..6257
                     /locus_tag="KPK87_001681"
     CDS             5331..6257
                     /locus_tag="KPK87_001681"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBG2537835.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(6309..7199)
                     /locus_tag="KPK87_001682"
     CDS             complement(6309..7199)
                     /locus_tag="KPK87_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2537836.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7387..8418
                     /locus_tag="KPK87_001683"
     CDS             7387..8418
                     /locus_tag="KPK87_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG2537837.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            8712..9587
                     /locus_tag="KPK87_001684"
     CDS             8712..9587
                     /locus_tag="KPK87_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBG2537838.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYAEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9605..11095
                     /gene="cls"
                     /locus_tag="KPK87_001685"
     CDS             9605..11095
                     /gene="cls"
                     /locus_tag="KPK87_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG2537839.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSYCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11288..11572
                     /locus_tag="KPK87_001686"
     CDS             11288..11572
                     /locus_tag="KPK87_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG2537840.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11605..13233
                     /gene="groL"
                     /locus_tag="KPK87_001687"
     CDS             11605..13233
                     /gene="groL"
                     /locus_tag="KPK87_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG2537841.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            13495..14235
                     /gene="larB"
                     /locus_tag="KPK87_001688"
     CDS             13495..14235
                     /gene="larB"
                     /locus_tag="KPK87_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG2537842.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14319..15053
                     /gene="larC"
                     /locus_tag="KPK87_001689"
     CDS             14319..15053
                     /gene="larC"
                     /locus_tag="KPK87_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG2537843.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            15083..15502
                     /locus_tag="KPK87_001690"
     CDS             15083..15502
                     /locus_tag="KPK87_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG2537844.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15562..15663
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15884..17419
                     /gene="guaA"
                     /locus_tag="KPK87_001691"
     CDS             15884..17419
                     /gene="guaA"
                     /locus_tag="KPK87_001691"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG2537845.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            17958..19388
                     /locus_tag="KPK87_001692"
     CDS             17958..19388
                     /locus_tag="KPK87_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2537846.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDID
                     NNTERYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(19536..19886)
                     /locus_tag="KPK87_001693"
                     /pseudo
     CDS             complement(19536..19886)
                     /locus_tag="KPK87_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            19881..20402
                     /locus_tag="KPK87_001694"
     CDS             19881..20402
                     /locus_tag="KPK87_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2537847.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(20531..20767)
                     /locus_tag="KPK87_001695"
     CDS             complement(20531..20767)
                     /locus_tag="KPK87_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537848.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFIENQ"
     gene            complement(21077..24505)
                     /gene="uvrA"
                     /locus_tag="KPK87_001696"
     CDS             complement(21077..24505)
                     /gene="uvrA"
                     /locus_tag="KPK87_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2537849.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLKKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCQQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
BASE COUNT         9463 a         2784 c         4378 g         8179 t
ORIGIN      
        1 atttaaaaaa gttagtaaaa aatttaacta ttgtatatta atgatagttg ctattgatat
       61 attatctaaa aaaattaagt atgaaaagat tactgctagg acaatctttt catacttaat
      121 ttttaatatt tatattatcc aaaattttca tctttttaac ttagaatttg agaatcacta
      181 ttaggaaata ggctttgatt tatgtagggt taaaaatttc acagaatatc taactttata
      241 ctaaatccat caaaaaatat acgccatata aaagcgcata aaaaaacaca taaaacaata
      301 tttatgcgca ctttaacctc gaatggtaca aaattcttta cttttacaga cccatattat
      361 ataattaagt aaataatgta tatttaggtg acatattgga taagctaatt tatttatttt
      421 attaatataa taatactata aaagttatat aaagtggttt acaaaaaagt tacagataga
      481 gcttttatta atgtattgtg tattagtatt ttatccttag gtacacttca cctacaaaat
      541 agaatcataa gataattatg ggaaaatttt aggaggttta tcgtgaagaa aaagatatta
      601 ataccagtat ttgcatcagt tatggcatta tcagtaagtt caattgtaaa tgcagatgaa
      661 gtaaatgatt catctcaaaa taaggatgat aaaacaaata ctgaattaaa tttaggtgaa
      721 tataaagaag taaaatataa agttgcaaaa ataaaagatg gagtagctat aaagataaga
      781 gaagaaggtc aagttcaaaa catagcttat tctggagatg aatttacggt tttaggaact
      841 caaggagaat gggttaaggt taaagttgaa gatggcgaag gttggttagc tactagatat
      901 gtagatatat ctgaaggtgt aggatatact aacgctgata aagtaaactt aagaaaagat
      961 aagagtgaaa gttcagaagt aattgaagag ctggaaaaag gaagttcttt attggttttg
     1021 gaagataatg gagattggtt aaaagttaaa gatggggaaa cagaaggata tgtaaaatca
     1081 tcttatatat ctgataaagc tcctgtaata gaagagccac aagtaaaccc aagtattgac
     1141 caaaatgtag attctacaaa taataatcaa caaaacaatg caaatactaa tcaacaaaat
     1201 aataattcaa atgtgccaac agctaactct aatgcagttc aagctgtact taacttggca
     1261 tattctaagc aaggttgtcc atatgtttgg ggagcagaag gaccaaacac ttttgactgt
     1321 tctggattta cacaatatgt gtatagaaat gcagtaggaa agaatatacc aagaacatct
     1381 aaagcacaat ctaaatatgg acaaactgta agtaaagcaa atttacaacc aggagactta
     1441 gtattcttca caactaatgg ttctggttct gttagtcatg ttggaatata tgtaggtggg
     1501 ggtaatatga tacattcacc atcaacagga aaaacagtta gtgtaactag tataaattca
     1561 tcatactata cagcaagatt tgttacagca aaaagaatat tataatcggt ttgtaatatg
     1621 acaaattgat aaatacataa atatatataa acgctctata ttatatttaa tttaatcatg
     1681 gaaacaaaaa atgattatat tttaaatgta ctatagagcg tttttgtatc actttaaatc
     1741 taatcaagat tcaagttaat atgactgtta aataggagcc ttttcatatg atgattattt
     1801 gcgcaatccc ttgacaggat agggcaaagg tggtaagcta ctaacagtac aaaaatactt
     1861 ttaattttag ttcagagagg ctaaaaaagg aggacgaaag atgttaaaat caagaaattt
     1921 aatccaacca gaagactttt caatagaaga aatagatgaa atattagact tagctcaaaa
     1981 aataattgac aatccgtcaa aatattctcg tatatgtgaa ggaaaactat tagctacatt
     2041 attttatgaa ccatcaacaa gaacaagatt aagctttgaa tctgctatga acagattagg
     2101 aggacgtgta gtaggctttt cagagccaaa ttcatcctct gcatcaaaag gtgaaactct
     2161 aggagataca atgaggatag tatctggtta tgttgatata atagctatga gacatcctca
     2221 atcaggtgca gcttcagaag cagctagata tacggaagtg ccatttataa atgcaggaga
     2281 cggcaagaac cagcatccaa ctcaaacact tacagactta cttactataa aatcattaaa
     2341 aggaacttta gaaagccata caataggatt atgtggtgat ttaaaatatg gaagaactgt
     2401 gcattctttg gttaaggcta tggcaagata taaaaataca aagtttgtat ttatagcgcc
     2461 tgaagagtta aaaatgccag attatataaa agaagctata aagggacatg catattatga
     2521 aactaataat ttggatgatg ttataggaag cttggatgtt ttatatatga ctagagttca
     2581 acaagaaaga tttgaagata aatcagagta tgaaagactt aaaaactatt atattttaaa
     2641 taaagctaaa ttagaaaagg catctaaaga tatgttagta atgcatccat taccaagagt
     2701 aaatgaaata gacatagatg tagatagtga tgatagagct gtatatttta aacaagctaa
     2761 atatggaatg tatgttagaa tggctttaat aataaaactt ttaggtataa atgaagacta
     2821 atagttatca tataatagta aaacaaagga gattttggga tgtataaaat tctggaaaat
     2881 atatatatag gcgaagatat gtatagaatg aaagtaaaag gtaattttga aggtaaaatg
     2941 ggacaattct atatgttaag agcatgggat acttatccag tactttcaag acctataagt
     3001 atacatgata tagatgaaga agggataact tttctttata aagtagtagg agaaggaact
     3061 caaattttga gtaacctaaa ggtaaatgat aatataaaat tagaaggccc ttatggtaat
     3121 ggatatgcta aggttgatgg aaaggtagct ctagtaggag gaggaatcgg tgtagctcca
     3181 ctatatttgg ttgctaaaaa tataaaaaat tgtgatgcat atcttgggtt tagagaagat
     3241 gttatattag aagatgaata taaacaagta tgtaataaag tctatacaac agtaggaaat
     3301 acttttgtaa cagatataat tgatgttgag aaatatgatt atatattaac atgtggtcca
     3361 actccaatga tggaaaaatt agttaagatg gtagaaggta ctaaaacgag aattatggta
     3421 tctcttgaaa accatatggc ttgtggagta ggtgcctgtt tagtatgtac gtgtaaaact
     3481 aatggaggaa ataaaaagac ttgtaaagat ggtccagtgt tctggggaga ggacgtgatt
     3541 tttaatgggt aatttaagtg tgaaatttgg aagtgtagaa tttaaaaatc cagtaataat
     3601 ggcgtctgga acttttggat ttggaaaaga atataatgag atttatgata tacaaaaact
     3661 tggtggtata agtagcaagg gtcttacttt aaataaaaaa cctggtaaca atggtatgag
     3721 agttcatgag acatcatccg gtatgatgaa tagtgttggg cttgaaaacc ctggagttca
     3781 aggatttata gactatgaat tacctttttt tagtaaacta gacttagtca gaatagctaa
     3841 tgttggaggt gggacattag aagactactt attaggggtt caaatgttaa atgacaagcc
     3901 tatagacatt attgaattaa atatatcttg cccaaatgtc aaagctggtg gaatggcttt
     3961 tggtataaaa aatgaagtgg caagagaagt tgttcgtgag gtaagaaaca taacgaaact
     4021 gcctttagta ataaaattat cacccaatgc agaagatatt gtaggaatgg caaaagtttg
     4081 tgaagaagaa ggtgctgatg gagtttcatt agttaatact tttaaagcaa tggcaattga
     4141 tataaaaaat agaagaccag tatttgaaaa tgtatatgct gggttatctg gaccagctat
     4201 aaaacctata gctttgagaa tggtacatga agtgtgtaaa aatgtaaata tacctgtaat
     4261 ggggatgggc ggaataacta aagccacaga tgctatagag tttataatgg caggagctac
     4321 ttgtatccaa gttggaactg caaattttat gaatcctaga ataggtattg aaataataga
     4381 tggaattaat gaatttatgg atagagaagg aataaaaagt ttagacgaaa taagaggaat
     4441 tatataaggt agaataaata gtattttaaa gataaaaaat atagattaaa taaacaataa
     4501 ttttataaca ttaacatgga ggaaaaataa tatgagtaaa gtagatgtag tggatatatt
     4561 aaagaaaagt gatgcattgt tggagggaca ttttttatta tcttcaggaa aacacagtaa
     4621 tagatacgta caatgtgcaa aagtattaag atttccagaa tatgctgctg aggtattaag
     4681 tacagttgtt gaacaaataa aagacttaga tatagactta gtagtaggac cagctatggg
     4741 tggagtaata gtttcttatg agttaggaag acaattagga aaagaagctg tatttactga
     4801 gagaaaagac aatacaatgg agttaagaag aggatttgaa gttaaaaaag gagcaaagat
     4861 aataattgct gaagacgttg taactactgg taaatcaact atggagacaa aaagagtatt
     4921 agaagcctta ggtggagaag ttgtaggtgt tgcatgtata gcagatagaa ctaatcatga
     4981 tataggtatg cctatatata gtgctataaa acttgatatt caagtttatg aatctgatga
     5041 gtgtccttta tgtaaggaag gaaaattacc agttgttaag cctggaagta gggagttcaa
     5101 ggaattaggg atgtaataaa aacttaatta tttatagtgt tacttaaaaa atgtaaattt
     5161 tttagagtaa aaatttgtaa tataacatat aacttaaaaa taaaaagcag ttagccttat
     5221 aaatgaaggt tagctgtttt ttttgttaaa aatatattta caaaataaga gatagtagca
     5281 gtacaatata agtataaggt aaaagtatta tatattaggg aggggtaagt atgaaaaatc
     5341 aattaaatga aaatcataaa aaggttgtat tttttggagt aggtgctgta ggagctactt
     5401 ttgcagaaca attttttaac tctaaatatg attttaaaat tctttgtgat aatgaaagaa
     5461 agaaaagata tttagaagaa ggatttataa taaatggaaa aaggtatgat tttgattatg
     5521 taactaaaga tgagtataaa caagaggctg attttataat tataggtcta aaatataata
     5581 atttaaaaga aaatataaaa gaattagatg gattagttgg aaaaaataca gttataatgt
     5641 ctctgctaaa tggagttgat agcgaagaga taataggaga aagatttgga attgaaaaaa
     5701 tggtatattc atatgttacc aatatagatg caaagaaaat caataataat attatacaca
     5761 ctactaatgg gataattgta tttggtaaca aagataatag tgaagataga aaaactaata
     5821 taataaccga agtctttgat gatgtaaata tagaatatac tttatcaaaa gatattcaac
     5881 gagatatgtg gtggaagtac atggttaata ttggtgtaaa tcaaacttca gctatacttg
     5941 gtgcacctta tggagttttt cagagttctg agcatttaag agaattagca aaatctgcaa
     6001 tgagggaagt tgttgctata gcacaagcaa aagacatatc tcttacagaa gatgatgtgg
     6061 aacattcatt acatagaata ctagaacatt caaaagaagg aagaacatca atgcttcaag
     6121 atgtggaagc tcatagactt acagaagtag atatgttttc taagaatatc tgcaaacttg
     6181 gaaaaaaata taacatacct actcctataa atcagacttt cttttatatg ataaaagtaa
     6241 ttgaaagtag attttaaagg gtctttgtaa gaaatattga taattattga ttttaaattt
     6301 tattaatgtt atatactatt taagttacta aatattttta actcttcaat gaaaatagtt
     6361 gctgcttttg ataaagttat atcttttgga aatatatatg agaaatgtct gttgtagctg
     6421 cttccaagct ctatgacaga cagctcattg tttagaacag gtaagcttgt cactagatta
     6481 gagactattg taattcctaa attatttcta acagattctt tgacagcata attacttcca
     6541 aaaaccatca tactttttgg aataatttct ttaacactta aaaacatatc taaataatct
     6601 ctggttccag aaccatcctc cctgactatc catttttggt tttgaagttt atcaaaacta
     6661 aaatctttta ataaatgact cttgtatgga agtgctaaaa ccatcttatc ttcaaaaaaa
     6721 tattcttgaa taaatgaagg ggaagagcaa gtgccttcaa taagaccaat gtctaaaata
     6781 taatccttaa catgagaaga tacgattgat gtatttttta taaaaacttc tacatctatg
     6841 tcaggatact ttttagaaaa taatgctaga aaatttggta aaatatactc tccaatagtt
     6901 aaacttgcac caatctttaa gtgaccagtt atagcatctg aaacttggct tacatcatga
     6961 taagtaatat ttaaaagatt taatatttct ttagctcttt tgtacaagat ttgtccactt
     7021 tctgttataa aaatggtttt ttgtttaatg gacctgttta taagaacaac tttaaaataa
     7081 ttttctagat tttttatgtg tttacttaca cttggttgtg atagatttaa atattcccca
     7141 gctttagtaa agtttttgta ttcaacaaca gctacaaatg tttttaattc ttcaaacaat
     7201 tttttgtctc ctttacctaa tgaaatttta cattttttat atactttcta cagataatta
     7261 tactatatat aataaccatt aaaaacatta atcattacga tattatatat ttattttact
     7321 tatggttatt acaattatat actattgcta tgaagaaaac tatattaatt ataaggtagg
     7381 tatattatgt taaagaaaaa aagcagtttg acattaaaaa ctataaggga aatattacca
     7441 gggctgtttg tatcagtatt agtaggttat attagtatat ttatttctat tttaattcct
     7501 aaggtagggg cagcatcaat ttctatattt ttaggtatgt ttgtaggaaa cctattttta
     7561 aatcaaaagg tatttcaaaa aggttataaa ttttcagaga cagatttgtt atcatattca
     7621 atagtactat taggagcaac actcagtgta tctaccttaa tagacttaaa agtttcagga
     7681 attttattta taatattgca aatgacaata acaataatag ctgcacttta tataggtaaa
     7741 aaactaggat ttgaagaaaa ctttagattt ctaatggcaa gtggaaatgc agtatgtggt
     7801 tcttctgcaa taggagcaac agttccagtg gttaatgcga cagataaaga aaagggaatt
     7861 gcagtaacta ttgtaaatgt tactggtata tttcttatgt ttttacttcc aataatatct
     7921 caatttttat atagccatga acttgtaaaa acttcagcaa tgataggtgg aactttgcaa
     7981 tcaattggtc aggttgtagc aagtggagca attgttggag aaaatgtaaa agatttagct
     8041 actattttta agatagtaag agtaatattt ctagttgttg ttgtattagt atttggacat
     8101 atgaaaaatc aatctaatag ggaaattctt gaagaagaaa aggatgagat aaaaaagaaa
     8161 aaagtgacta taccatggta tgtaattgga ttctttataa cttgtgcact tttttcaatg
     8221 aacattatac cacaagaagt atctgtgttg tgcaaagaaa taagtaacaa actagaaata
     8281 atagctttag cagcaattgg attaaaggtt aatgtaaaag atttagtgaa acaagggaaa
     8341 gaagtttctt tatatggttt atttgtaggt acagtacaag ttgtatcagc tgtagtttta
     8401 ataaaaatat ttatatagca taaaaatagt atttgtaata ttaatttgat taactaaaat
     8461 aatatgatac taataattta taatataaat aaaaaatgaa tttcgataag tttaagtggg
     8521 tataaactta tagaaattca ttttttattt aaggtaaaaa tttaaagtaa aatattattt
     8581 tacataataa taaccttata aattttgaaa ttacttatct ttgtgttaaa ataagtaaaa
     8641 tgcacatatt agagctaagt ttttaagtaa ataaacaaaa tgaaaatata aatattaagg
     8701 agatttggta tatgaatgtt tatgaaaaag gtaatggagt tatactagaa ggagtaacag
     8761 attttgaccc agtacatata tttgagtgtg gtcaatgttt tagatggcac aaacaagaag
     8821 atggctcata cactggagtt gcaaagggga gaatattaaa tgtcaaaaaa gagaatgata
     8881 aaatatattt aaataatact aatttaaagg agtttaacag catttggtat aattactttg
     8941 atttaggaac tgattatgca gaaataaaga ataagctaaa aaatatggat gaatatttaa
     9001 ataaggctac tgaatttgga tggggaataa gaatattaag acaagatgga tgggaaatgc
     9061 ttatatcatt tataatttcc tctaataata ggattcctat gatacaaaga gcgatagaaa
     9121 atttatcaag aaagtttggt aagtatatag gtgaatatga aggcaatgaa tactatgcat
     9181 tcccaacacc agaagaacta aataaggcct cacaagagga aataagagca tgtcaaacag
     9241 gttttagaga taagtacata aaaagtacta cacaggcagt tatagagaat aatgatgagg
     9301 tatctgaata tacaaattta agtacagagg attgtagaaa agagctttta aaatttaatg
     9361 gagtaggacc aaaagtatgt gattgtattg cattatttgg tatgcaaaaa tatgattctt
     9421 ttccagttga tgtttgggtt aagagagtta tgcaagaatt ttatatagat gaagacatga
     9481 gtttacctaa aatgagaact tatggaatcg ataaatttaa agaaatgtct ggatttgctc
     9541 aacagtactt attttactat gcaagagaac tcggtatagg aaaataaatt aagggggaga
     9601 aattatggga gtaataggaa caatatttct gttctattta attatatcat atctagctgg
     9661 agccataatt tctgtgatta tattacttga aaatagagac cctgcaaaaa caatgtcttg
     9721 gctattgatg tttattatat ttccaggtgt aggtttaatg atatatgcta tttcaggtag
     9781 aaatattagg aaaagaaaat tatttaagac tcaaaaacta gctaataata taaaagaaaa
     9841 gaaactattt gatacattag aaaagataac agaaatagtt gaattagaaa aagaatctat
     9901 aaaacaaaat aagcttttaa gagatgaaga ggatggtagt tatagaaaaa gggtaataaa
     9961 tatgttgtta aagactggta tgtttccctt tactaaaaat aataaagtgg atgtatttgt
    10021 agatggaaat gaaaagttta aaagacttat agaagatata agagaagcta aagaccatat
    10081 tcatttagag tactttatca tcaaagatag tgaaataggt agggtgttaa aagaagaact
    10141 tataaaaaag gctaaagaag gcattaagat aagaatttta tatgatgatg taggatgctg
    10201 gagattttgg tttaatagaa aatttttccg agaaatgagg gaagtaggta tagaaattgc
    10261 agcattttta cctacaaaat ttcctataat aggagggaaa ttaaactata gaaatcacag
    10321 aaaaattgtt gttattgatg gaataattgg atatacaggt ggaataaata taggagatga
    10381 atatttaggc aaaaatgata agtttggata ttggagagat actcatatta ggattaaggg
    10441 tatttctgta tacatgcttc aaatgacatt tctgattgat tggtattata cgacaaaaga
    10501 agtattagtg actaaaaatt atttcccaag tgtagggaat gttggtgaaa gtatgataca
    10561 agttgttgcg agtggtccag acagtgactg ggaagatatt cattatgctt atttttctgc
    10621 aatatgtcag gctagaaaaa atgtttatat agaaactcca tattttattc cagatgaaag
    10681 tctactaaaa gctataaaga gtgctgctct tagtggagtt gatgtgagaa taatatttcc
    10741 taaaattgca gaccacaaga tagtaaatat agcatcttat tcatattttg aagaaatatt
    10801 aagagcaggt ggtaaagttt atttatataa taaaggattt atacattcta aagtcgtaat
    10861 aatagatgat aaaattgctt ctgcaggtac agctaatatg gatttaagaa gttttatgct
    10921 taactttgag gtaaatgcat ttatctatga tgaagaagtt attagggtaa tgacagatga
    10981 tttctttgag gatttaagtt actgtgaaga acttaatttg gaggtattta agaatagaaa
    11041 tatcatacaa aaaatcaaag aatctgtagc aagattattt tcaccaatat tataaaaaat
    11101 tattaaaaaa ggtattgaaa attatgtgtg attggtatat tataataatt gttagcactc
    11161 tattagatag agtgataata aataaaaagt atatatataa atctacatat ataagtagta
    11221 ttaatttaaa aataactaaa aacataggcg aactagtcta tacttaatat tttaggaggc
    11281 gtgaataatg aaaataagac cattagctga cagagtagta attaaaaaag tagaagcaga
    11341 agaaaaaact gcaagtggaa tagttttacc aggagcagct aaagagcaac ctcaaatagc
    11401 tgaagttgta gaagttggac caggtggaat agttgaaggg aaagaaataa aaatggaatt
    11461 gacagtggga gataaggtta tattccaaaa atattctgga acagaagtta agatagaagg
    11521 acaagaatac acaatactaa gacagagtga tgtattagct gttattgaat aaatatagaa
    11581 taaatttatt aggaggggtt taaaatggct aaagaaatta aattttcaga ggaaacaaga
    11641 agagctttag aagctggtgt aaataaatta gcagatacag taaaagtaac attaggacct
    11701 aaaggaagaa atgttatatt agataaaaaa tttggttctc cacttataac taatgatgga
    11761 gtaactatag caaaagagat agaactagaa gatagatttg aaaatatggg tgctcaatta
    11821 gttaaagaag tagcaactaa aactaatgat gtagctggag atggtactac aactgctaca
    11881 gttttagcac aagctataat aagagaaggt ttaaagaatg taacagcagg ggctaaccca
    11941 atacttttaa gaaaaggaat acaaaaagca gtaacagtag cagtagaaga attaaaaaat
    12001 caatcaagaa tagtagaaac acaagaggct atatctcaag ttgcttctat atctgctggt
    12061 gatgaagaag ttggaaaatt aatagcagaa gctatggaaa tagtaggtaa agatggagtt
    12121 ataactgttg aagagtctca aactatgaat actgaattag atgctgttga aggtatgcag
    12181 tttgatagag gatttgtttc tgcatatatg gttacagatg tagataaaat ggaagcagtt
    12241 ttaaatgacc catatatatt aattactgat aaaaaaatat ctaacataca agaattatta
    12301 ccagttcttg aacaaatagt tcaacaaggt aaaaagttat tgataatagc tgaagatgta
    12361 gaaggtgaag cattatctac attagtagta aataaattaa gaggaacatt tgatgtagta
    12421 gcagttaaag ctccaggctt tggagataga agaaaagaaa tgcttcaaga tatagcaata
    12481 cttacaggtg ctcaagtaat atcagaagaa ttaggttatg atttaaaaga agctgattta
    12541 tctatgctag gtagagcttc atctgttaag gtaactaaag aaagtactac aatagtagat
    12601 ggttctggag ataaaaaagc tatagaagat agagtaactc aaattaaaca tcaagtagag
    12661 caaactactt cagattttga tagagagaaa ttaatggaaa gattagctaa acttgctgga
    12721 ggagtagctg ttgtaaaagt tggagctgct acagaagtag agttaaaaga aagaaagtta
    12781 agaatagaag atgctcttaa tgcaactaga gcagctgtag aagaaggaat agtagctggt
    12841 ggaggaactg cttttgttag tgttatacca gcaataggaa cactaattga gagtttagaa
    12901 ggagaagtta aactaggtgc tcaaatagtt aaaaaagcgt tagaagagcc attaagacaa
    12961 atagctataa atgctggtct tgaaggtgct gtaattgtgc aaaatgttgt taattctgaa
    13021 gcagaaactg gatttgacgc attgaatgaa aaatatgtaa atatgataga agctggtata
    13081 gttgacccaa ctaaagttag tagaagtgct ttacaaaatg ctgcatcaat agcaagcact
    13141 ttcttaacta ctgaagctgc tgttgctgac cttcctgaaa aagaagatgc aggaatgcca
    13201 ggaatgggtg gcggaatgcc tggtatgatg taatttaaaa aatttaaaat ataaatttta
    13261 atataaagaa ctatttcgac ttagatttta aactctaagt tgaaatagtt cttttgtata
    13321 tatctaattt ttgtatatct ataaattcaa tacacatatt tagataatca gtatctagtt
    13381 tgtaataata ttttagagta gtattgttat tttttaggtt atgattttag atatatatta
    13441 attaaggtat aattgttgat agagaataaa tcaaatttaa aaggatgcga taatatggac
    13501 ttaagaaaat tgcttgaaga agttaaaaat gaaaatattg atatagattt tgcgttagaa
    13561 aagttaaaag atttacctta tgaagacctt ggatatgcaa atatagatca tcatagagaa
    13621 ctaagaaatg ggtatccgga agttatttat tgtgaaggta agagtgatga gcatatttta
    13681 gggattattg ataaaatgaa tcaaaaaagt tctaacatat tgggaacaag gtgcagaaaa
    13741 gaaacttttc tgaaaattaa agaaatctat aatcatgctg agtacgaaga ggcgtctaaa
    13801 attcttaaga ttcaaaatca tgatatagag aatataggaa aaggtaaaat tgtgattgct
    13861 acagggggaa cttctgatat accagtagca gacgaagctt atcatacagc taagttttta
    13921 ggtaatgatg ttgatagaat atatgatgtt ggagtggcag gtatacatag gcttttaaat
    13981 aaaagacata aaatagatag tgctagagta atagtagccg ttgcaggtat ggaaggtgct
    14041 cttgcaagtg taattggtgg actggttgat gttccagtaa tagctgttcc aacttctgtt
    14101 ggatatggag caaattttgg aggattagca gcattactag ctatgttaaa tagctgtgca
    14161 tctggaatat ctgttgtaaa tattgataat ggatttggag caggatattt agcatcaatg
    14221 ataaataaac tgtaaataca ttgatataaa ttaaacaaaa ctatataata aaatttaatt
    14281 tctattatat ttttactatt aaaattgagg agacatatat gagtgaaaaa atactttatt
    14341 ttgatataat aagcggtata tcaggggata tgacccttgc aagtttatta aatttaggtg
    14401 tgcctaagga aatttttttg gaggaaattc ataaactaag aatgagcgat gaatttaata
    14461 taaacatcag ctcgaaaact gaaaatggaa tagtaggtac aaaagtagaa gtaattacaa
    14521 aagaaaaaca tacacataga aatttagtgg atatatttga ggttatagat aaaagcactc
    14581 taaacgaaaa tgtaaaatct aaagcaaaaa aaatatttat ggtaattgga gaagcagaag
    14641 caaaagttca tggaactaca atagacaaga tacattttca tgaggtaggg gctatagatt
    14701 caattgtaga tatagtagga gcttctatat tagttgactt actttgtgta gataaagttt
    14761 gtgctagtac agtgtctgtt ggttcaggat ttgtaaaatg tgaacatgga attataccta
    14821 tacctgcacc tgcaactata gaaattttaa aaggtgtgcc aattaaatta aataatgtaa
    14881 atggagaatg tacaactcca acaggagctg caatcataaa agttttgtgt gatgagtttg
    14941 tagatgagct tgaattttct ccaaagcaaa ttggatatgg tataggtcat aagaagtttg
    15001 aagtaccaaa tatactcaga actgtattag gtgaaaaaaa aaagaagaat taatttatga
    15061 aattagtgca aacattgatg atatgtcttc agaaatctat tcttatttgt atgaaaaaat
    15121 attagatgag aaagcattag atatttatac agagagcatt tatatgaaga agaatagacc
    15181 agcaacaaaa gtatcaattt tatgtaatga atcagattta gaaaaattta ttaaaatatt
    15241 acttttagaa actagtactt ttggagtgag atataatgca tataatagag aaattctaga
    15301 gagaaaattt gtaaaattag atacaaaata tggagtggtt acagtaaaac tagggtatta
    15361 taaaggaaaa ctagtcaagg ctactccaga atataatgaa tgtaaaacta tagcaaaaaa
    15421 tgaagatatt cctataaaaa aagtgtttgc tgatataaat tttagaataa aagaagaatt
    15481 tagtgaaaac ttattgacat aagtaataaa ttttgataaa ttaatattca gccttattaa
    15541 ataaaagtaa acagaaaaaa atatataaaa tataaactca ctcgtatatg ctcagaaata
    15601 tggtctgaga gtctctacca agataccgta aatatcttga ctatgagtga aattattata
    15661 ccaaaagacc tagttaagta aatgtgtagg ctgagttaaa ctccctatgt gttggctata
    15721 agataaaggt attttggaag cctgtatagt aatttcactt ttgctagaaa ttatggaagt
    15781 tactatacag gttttttgtg taaaaagatt taatataatt tagactgtat tgtgttaaaa
    15841 taaatatata aaaactatat tatacaagga gagatgtgaa tttatgaaac atgaattagt
    15901 acttgtaata gattttggag gacaatataa tcagttgata gctagaagag ttagagaaaa
    15961 caacgtatat tgtgaaatac ttccatgtac tgcaagcata gaaagaataa aagaaaaaaa
    16021 tcctaaaggg ataatattta caggcggtcc aaatagtgcg tatttagaag attctccaac
    16081 aatatcaaaa gaaatatttg aattaggagt accaatactt ggaatatgct atggtattca
    16141 aataatgtct catgtattgg gtggagttgt aagaaaaggt aataaacaag aaaaagaata
    16201 tggaaaaaca gctataactt atggaaaatc atcattattt gaaggaataa ctacaaatag
    16261 tgtatggatg agtcatacag atttaataga aaaagttcca gaaggattta ctatagttgc
    16321 aaatactaat gattgtccag tagcagctat ggaaaatgta gaacgaaacc tatatggagt
    16381 tcaattccat ccagaggtag aacactgttt ggaaggggac aagatactta caaacttctt
    16441 atataatatt tgtaaagtaa aaggtgactg gactacagat tcattcatag aagataaaat
    16501 aaaagaatta aaagaaaaaa taggcgataa aaaagcttta tgtgctttaa gtggaggagt
    16561 tgattcttct gttgctgcag ttcttattca taaagcaatt ggggacaact taacttgtat
    16621 atttgttgac catggtttac ttagaaaaaa tgaaggaaat gatgtagaaa gaatatttag
    16681 agagaaattt gatataaatt taataagagt taatgcagaa gatagattct tatctaaatt
    16741 aaaaggagtt tctgaaccag aagctaagag aaaaataata ggtgaagaat ttataagagt
    16801 atttgaagaa gaatcaaata aattaggaaa aatggatttc ttagtacaag gaacaatcta
    16861 tccagatgtt atagaaagtg gacatggaaa tgctgctact ataaagtctc atcataatgt
    16921 tggtggaatc ccagaagatg tagacttcca agaaattgta gaaccattaa gagagttatt
    16981 taaggatgaa gttagaaaaa taggattaga gcttggtata gaagaaggat taatatttag
    17041 acatcctttc ccaggaccag gtctaggaat aagagttata ggtgatgtaa ctaaagaaaa
    17101 atgtgatata ttaagagaag ctgatgctgt ttatatggac gagcttagaa aagcaggttt
    17161 atacagagaa atatggcaag cttttgcaac attaccagat gttaagactg ttggagttat
    17221 gggtgatgaa agaacatatg cttatttagt tggtttaaga gcagttactt cttctgatgg
    17281 aatgacttct gactggtata agatgcctta tgatgtacta gaaagaattt ctaatagaat
    17341 aataaatgaa gttgatggag taaatagagt agtttatgat ataacttcta agcctcctgg
    17401 aactattgaa tgggaataaa atatagaatg ccaaaaatcc acataaatac aggatttgag
    17461 gtctatctta cttctatgtt atactggttt gatactatca atatttgcca gtatcttatg
    17521 gattaataag caaaaaacat cctgctgaat gataaattca gcggggtgtt tttattttgt
    17581 tttagaactc ctttgctctg aaactcgaag tatttaacac tgatgatttt gttatctttt
    17641 agttcctttt gtgtcatgct ctgaaagaca aattgataca ataaaaaagt aatttcttaa
    17701 tcattgatgg agatatgtat ttatgttact cttgatggta atttgagtaa tattgttcat
    17761 agctttttat attcacttaa ttagaaaatc atttggtata atcataaata aacttgatga
    17821 tataatagaa aagatttata attattagaa gtatataaaa gtattttatg tctatgaaat
    17881 gtatgtctat aaaacatata gatgactttt gtatattaat gtatgaatat tgatattata
    17941 gaagaattga gttgtcaatg atactaaatc aaatatttat agaagctgga gggattatga
    18001 gaaataaaat aattatagga attacttttt cacttgtatt actaatgtct ggatgtgctt
    18061 caaaacaaac cgcagaaata aacgtattag caactactga tttacatgga gttatacctt
    18121 atgagttaac ttcttatgta aaagaagaaa gaaaaaaaga taaaaatcta actttagtag
    18181 atgcaggaga tttttttgat agtggagaag tgtttggttc tagcatggat agttatttta
    18241 gtgctaggta taaggatatt gataataaca ctgagcgtta cattgagaca cctatttcaa
    18301 aagatatgaa agaggtaggt tatgatgttg cagttcttgg gaatcatgaa tttatatcca
    18361 acaataaatt ttatctagat aatatgatat ctgattttga gaaacaaaaa ataaatattt
    18421 tatcggcaaa tacgtataaa aaaaatggag atagttatac aaaaccatac ataataaaaa
    18481 atattaatac acctgaagga aacgtaaaac taggaatttt agggcttact ataaaagaag
    18541 ttggtgagag gaaacagtgg aaagatggaa aacttgttga ctctaagtct ttagaactaa
    18601 aagaccaaga gggttacaat ggtgaattgt atatgaatga tttagtagaa gatgctaaaa
    18661 agtgggttgg tataatggaa aaagagaagc ctgacattat agttgctgtg gcacatacag
    18721 gtgagaaacc taagaaacct aaaaacccag gaaatagaat acaagactta gctcaacaag
    18781 ttgatggaat tgatgctata gttgcaggtc ataatcatgt tcaaattgag caacatgatt
    18841 ataaaaataa atcaggagaa aatgttatag ttactgagcc aggaaagcat ggggaatgta
    18901 tttctaagat taattttaaa ttagaaaaaa acaatgataa atggaatata gttgacaaat
    18961 ctagtaaatt aattaaattt gaaaaaaatc aagaaagtga taaatttgga gaatttattt
    19021 cttcaattaa taaagtaagt aaagataaga aagaaataag attatcagaa gtaaagactc
    19081 ctgaatggga taaaatatat gtttttaaat ctggaacatc tgcagatgat atatataaaa
    19141 caataggata taaatggaga aatatatcat ctccaaagag tgatggcatg gtgcagattg
    19201 ttgtaatgaa taataataag cctgttagct atatatatgg tgaagctgaa gttatgccaa
    19261 ttaatataaa atgtgatgaa tcagagtata aagataatgt tgtaactatt tatcctaata
    19321 aaaatgataa atttaaaatt gaaaaaggta aacaagaata tagtattaat ttaaaacata
    19381 tacaatagaa acttttatct tatttaagtt tagattgtac ttaaaataaa gtgtgtatta
    19441 ttttaaaaat tgatgataat taaaatcaca acattaagga atagtacaca ctttttatgc
    19501 taaaaaatag aatgttaaaa aattaaacct ttaaattaaa gtacattgct tatattatcc
    19561 aatattatct tcaaaggttg tgtaatattt ttatccttat gtaatattgc taacatttta
    19621 ttttccatat ctatcccatc taattcaatc aatcgcaatt ttttatgtaa gagtgaatct
    19681 aataataaat tttctggcaa aatggttata cctaacactg cttgtgcagc tttaataagt
    19741 gcaaaagaat taacactttc ccaaacagga taagcttttt ggtttgaaag agataataca
    19801 ctgtccaaag tatcgcgaat ggcacttcct tgctcacgta acaataacgg aaacgtgcat
    19861 aactgtcgta gagataaggc ttgtttagta ttggtggtgg ttatgcaatc atacctttaa
    19921 tccaagagaa agtagtaaat caaactggat ggataagtga gaaaatgttt acagatatta
    19981 taactatttc acaaatgaca ccagggccac tggctgttaa tacttctact tttgttggac
    20041 ttcaaattgg agggttttgg ggagcagttg cagccacttt aggttgtgta ttatgtggtg
    20101 tagtaatatc tctattttta tataattttt ttcaaaagca tcagaactct acttatatat
    20161 ttgaaatatt aaatggtcta aaatcagctt cattaggttt aattatctca gctgcggcta
    20221 caattataat ccttgcattg tttgggacga ataaagtgta cttcaattct atatttgaga
    20281 tgctaaactg gactgcacta ataatctttt ttgttacact actgatagta cgtaagtgga
    20341 aaattaatcc tattgtgatt ataataataa gtggtatact gggaatattt ttttatttat
    20401 aagtttttgc taagtaaagg atatctattt ttttatatct taaatccaaa tagccaataa
    20461 aataaggcta tttggatttt taataaattc tgaaaagata atcagaaacc tatataacat
    20521 ttaaattata tcactggttt tctataaacc aatcataagc ttcttggcaa aatttctcac
    20581 tatctatata gtaactatta tgaacattat taatttcaac aagtttcttt ttacatttta
    20641 gtgaattata atagctatca aaatattggg ttttaaaata tctatcatga agtgttttaa
    20701 agacaagagt aggtatgttg actccatctt ttatcttact atcttctgca ataaagcatt
    20761 ctaacattgt attaattatc tttgtactat ttttccaaat atgaggattt ttcttttcaa
    20821 atattttata aaattcatta tcatagactt cattataacc tttatattta ggtggtttta
    20881 ctgttatgtg aaaattagga agaaacttaa agaaaaaata taataatttt tatgttctgt
    20941 tttatatatt attcatattt tatcacagat aatatactaa agttaatttt tttattgtac
    21001 aactattata ataattcttc tataatataa gacattctat aattttatat ttaagttcat
    21061 gtgtatttaa ttgtttttat ataaaatgtc cagtaatact atttggattt atttttattt
    21121 catcaggagt tccagaagca acaatttccc ctccatttat tccactgtct ggacctatat
    21181 cgataatcca atcagaagca cgtataacct ctgtattgtg ttcaataatt aaaagtgaat
    21241 gctgttcatt tacaagtttt ctaaaagtaa ttaacaaatt ttctatatct ttaaaatgaa
    21301 gtccagtagt aggctcatcc aatatgtaca gcattcgttt tttagttttt gtccctaatt
    21361 cttttgctaa ttttatcctt tgagattctc ctccacttaa tcctaatgta ttttgtccaa
    21421 gctttagata tccaagtccc acttcatcta ataaggagag ttttctatat atatcagagc
    21481 aatcgctaaa aaatgttttt gcttctgcta cttccatgtc aagtatatct gcaattgtct
    21541 ttcccttgta ttgtatagac agtatttctt tttgatatct cttaccttga cattcactac
    21601 aagttaccca ttggtctgcc atatattgca gaataatttt tgttttacct aatccttgac
    21661 agtttgggca ttgccccttt ttgctattaa agctaaaata gctttcatcc aataattttt
    21721 gttttgcttg tggagtttca gcaaatagag aacgaatttc atcaaaaata ttgatatatg
    21781 tagcaggtgt agaacggcta gattttccaa taggtgtttg gtccattaag ataaaatcat
    21841 caatagcatc aaatccagta aattcagtat aatttttatc tgggatagat ttttgtttca
    21901 atttttcttc tagagcagga agtaaactgt gaaatacaag agagctctta ccagaaccac
    21961 taacacctgt aatagagcac atacagttta agggaatttc tacatcaata ttttttaagt
    22021 tatttgcatg gcatcctttt aagcttaacc atttacttgg tatagtttta ttttgtgaac
    22081 ctgttttatt gtatgtactt aaatatttac ctgttataga gtttggattt tttataattt
    22141 cttgggttgt accttctgca ataatgaacc ccccttttgt accagcactt gggcctacat
    22201 caataatata atcagcagac agtattgtat ctttatcgtg ttccacaact ataacagtat
    22261 tacccttatc tcttagttct tctatcattt tgatgattaa attatggtct cttgggtgta
    22321 aaccaataga tggttcatct aaaatgtagg ttaagcctac taattcactt cctagctgac
    22381 ttgataatcg tactctctgc agttctcctc cagataaagt tggagctgtt ctatctgttg
    22441 atatatagct aagacctacc tttaatagat aactaactcg aatttttaat tctgttaaga
    22501 tatcatttac caaagagagc tcattttttt gaagttgatt tggtaattca cacaaccaat
    22561 tccataactg ttctatcgtc atttttgtta gttcagggaa gcgataacct ttgattgtag
    22621 tgaatctagc ctcaatgcac agcaattcgc caccacatgt attgcaagga attttgttca
    22681 tatattgatg caaggtatta ttttcagaac tagattctct aaaaagtctt tgaatgtggt
    22741 tgattgctcc tgaagcaggt cttctaatct ttgattctcg tccctttgat tcaaagctaa
    22801 attcatatat cttatcgtct gtaccatata agattgcatc tataaaatct cttggtaatt
    22861 cattccaagg tatatctagg tctatatttt tatcttttgc tattgcatat aattcaccta
    22921 ccatccaatt accatttggc tttttccccc tcaattttcc aaaatagtaa gtggctccat
    22981 ctaatattga aatagttggg tcttttacaa tcaattgagg attaacttga taggtaaaac
    23041 ctaatccatt acaatcgtga caagctccag tatgggagtt agcattgaag gttgaagtat
    23101 ttatcttaga aaaaatagtt tgacaatgcg gacaataatt ttttgtatta ttcttttttt
    23161 ctaatggaat tttacaatat ggacagtaac tttttccgat tgatacaaat aacattctta
    23221 aataatccca aatgcttgta agagtaccaa cagtagagcg aggattttgg cttccttttt
    23281 tctgttcgat agctatagta ggggttaatc cctgaagtaa atcaaattcc ggtttttgaa
    23341 gctgttctga aagttttgct ccattactta gattttcaaa ataacgtctt tttccttctt
    23401 cataaatcgt atcgaaagct aaagaagatt ttccactacc acttacacct gtaataacag
    23461 ttatttgatt ttttggaatt gtaacctcaa catcctttaa attgttttgc cttcctcctt
    23521 ttaaatagat agagctttta ttatgacaaa gtgaattttt aattgattta tttattgatt
    23581 ttgtaaattt gaatttcttt tctagttcaa gaggattgcc taattctttt atttccgaaa
    23641 tacctaatat ttttaactga gaaaagattg atttcaaata atcttcaggc aatttttcta
    23701 ttaacaaatt aaggtcttct atttttatat ttccgcttga taatatctct gccatttcat
    23761 ctaataaatt tttttctgct cttgttaatt gattgtattt tttggaactt aattctgtcc
    23821 ataaatccca tggaagcaat tctaaaccaa tcatattatt aaagtcatgt agaagattac
    23881 cctttacaga atgccaacct ttccaatttt tattatatcc aaagtcatct ggatgagcat
    23941 gtccttgctg acatcttatc caggcttctg aaccagttaa aaaaccatct gtttttccga
    24001 catcatgagt gtcaaaatta atttgaaaat aatttttttg tatgtcatct atttgtgcat
    24061 ctattcttat ccatctcttt tttagagtat catagtattc tacaagccag tggtcttcat
    24121 aagtaagctc actttcaaaa taattagcaa aacctgctct cattctagcc tcaaaacctc
    24181 gatatcttag gagcgataca agaagtaaag aataatctct gcacattcca acaagccttt
    24241 gttttgggtt tcttggaata gttagtgggt tagaatttat ttcaaatatt ctttttaaca
    24301 tttgaggaat tgtacgaagc aattcctcat cagattgttg tttattaaag gatataccat
    24361 ataattttcc ttggtctcca tgaattacta ggctttgaac cacttttaat aactcaattg
    24421 gagtctttgg taaatcttga tatttatttt caaatttttt aggatttgtg atgatacttt
    24481 gatttttata gttatttatt ttcatttata tttcacctct ctaaatggat gatataccct
    24541 gacatggtgt catatgcaag gacaatttag attttttggc caaaatttaa aaataaataa
    24601 aaaatgatac agtgataaat tgtaatacaa atatagtttc aaattgcata taatataatt
    24661 atttaataaa accattatct attgaatgtt aatgaaatgt taagatggca ttcttgaata
    24721 ttaagaaata gaatactata atatagtgtg aaggattttt agtaaaaaaa tttgtaagtg
    24781 ttaatataca ttaaaaaata ggtt
//
LOCUS       DAEMIK010000050        24615 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEMIK010000050 DAEMIK010000000
VERSION     DAEMIK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.050"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            183..2282
                     /gene="nrdE"
                     /locus_tag="KPK87_001697"
     CDS             183..2282
                     /gene="nrdE"
                     /locus_tag="KPK87_001697"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG2537850.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2295..3266
                     /gene="nrdF"
                     /locus_tag="KPK87_001698"
     CDS             2295..3266
                     /gene="nrdF"
                     /locus_tag="KPK87_001698"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG2537851.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3342..3968
                     /locus_tag="KPK87_001699"
     CDS             3342..3968
                     /locus_tag="KPK87_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2537852.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4363..4992
                     /locus_tag="KPK87_001700"
     CDS             4363..4992
                     /locus_tag="KPK87_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537853.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            4993..5775
                     /locus_tag="KPK87_001701"
     CDS             4993..5775
                     /locus_tag="KPK87_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537854.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKEKMVEAYE"
     gene            5768..6646
                     /locus_tag="KPK87_001702"
     CDS             5768..6646
                     /locus_tag="KPK87_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537855.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            6679..7638
                     /locus_tag="KPK87_001703"
     CDS             6679..7638
                     /locus_tag="KPK87_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2537856.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            7952..8644
                     /locus_tag="KPK87_001704"
     CDS             7952..8644
                     /locus_tag="KPK87_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537857.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8654..9658
                     /locus_tag="KPK87_001705"
     CDS             8654..9658
                     /locus_tag="KPK87_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2537858.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            9906..10757
                     /locus_tag="KPK87_001706"
     CDS             9906..10757
                     /locus_tag="KPK87_001706"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG2537859.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDRVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKLLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            10861..11607
                     /locus_tag="KPK87_001707"
     CDS             10861..11607
                     /locus_tag="KPK87_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537860.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            11607..13523
                     /locus_tag="KPK87_001708"
     CDS             11607..13523
                     /locus_tag="KPK87_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537861.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGITYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            13802..14719
                     /locus_tag="KPK87_001709"
     CDS             13802..14719
                     /locus_tag="KPK87_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2537862.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEISVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            14797..15534
                     /gene="cas6"
                     /locus_tag="KPK87_001710"
     CDS             14797..15534
                     /gene="cas6"
                     /locus_tag="KPK87_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG2537863.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKSMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            15540..17417
                     /gene="cas8a1"
                     /locus_tag="KPK87_001711"
     CDS             15540..17417
                     /gene="cas8a1"
                     /locus_tag="KPK87_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG2537864.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRVLINSYKKG
                     VYKMDSKNLEKNDEKLKIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            17419..18333
                     /gene="cas7i"
                     /locus_tag="KPK87_001712"
     CDS             17419..18333
                     /gene="cas7i"
                     /locus_tag="KPK87_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG2537865.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRADGNMYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            18335..19036
                     /gene="cas5b"
                     /locus_tag="KPK87_001713"
     CDS             18335..19036
                     /gene="cas5b"
                     /locus_tag="KPK87_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG2537866.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKVLN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVDDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            19047..21368
                     /locus_tag="KPK87_001714"
     CDS             19047..21368
                     /locus_tag="KPK87_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG2537867.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTERLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            21383..21934
                     /gene="cas4"
                     /locus_tag="KPK87_001715"
     CDS             21383..21934
                     /gene="cas4"
                     /locus_tag="KPK87_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG2537868.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            21937..22917
                     /gene="cas1b"
                     /locus_tag="KPK87_001716"
     CDS             21937..22917
                     /gene="cas1b"
                     /locus_tag="KPK87_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG2537869.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            22920..23186
                     /gene="cas2"
                     /locus_tag="KPK87_001717"
     CDS             22920..23186
                     /gene="cas2"
                     /locus_tag="KPK87_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG2537870.1"
                     /translation="MFVIVTYDIVEARSLNRIRKILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   23370..24056
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=23370..23398
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            24245..>24615
                     /locus_tag="KPK87_001718"
     CDS             24245..>24615
                     /locus_tag="KPK87_001718"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG2537871.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIV"
BASE COUNT        10054 a         2251 c         4306 g         8004 t
ORIGIN      
        1 tatatgtagt atataataga taaagcactg actatatgta gtgcttttgt ttttgaaaaa
       61 tacttattgc atttctataa ttatagatta attttgggat ttcaactgat aagtaccaaa
      121 agaaataata agcaaattaa gtgaaattat ggcacaagat tatgttaaaa ggacggtaaa
      181 taatgaattg gatagagctt aacaatcaag tgataattaa aaatgaacaa ggcaagtacc
      241 aattggaaaa ggataaggaa gccttaagta gttacataga agaatttata cagccaaagt
      301 tgagaaaatt taataaccta gaagaaagat tgaagtattt aatagatgaa ggttattatt
      361 caaaagaagt aataaatcag tatagcatag atgttattaa aaatatatat gatatgattg
      421 ataaacataa gtttgaattt caatcatata tgagtgcaaa taagttttat caaaattatg
      481 cattaaagag caatgatgga aaggaaatat tagaaacata taatgataaa gtgttaattg
      541 tagctttgac actaggaaat ggcgataaag atttagcttt aaacttggca gataaaatag
      601 taaaacaaga atttcaacca gctacaccta cttttttaaa tgctggaaga aaaagggctg
      661 gagaaatggt atcatgtttt ttactatctg tagaagatag tacagaaggt atatcgtatg
      721 ctatatcttc atcaaatcac ttatcaaaga ttggtggagg agttgcacta aatttatcta
      781 aacttagagc atctggcgaa tctatcaaag atatagaagg tgcagctgga ggtgttgttg
      841 gagtagctaa aatgttagaa caatcattta gttactttaa tcaaatggga gctagacaag
      901 gttctggtgc ggtgtacctt actgtattcc atcctgattt tgaattgttg atggatacta
      961 aaaagataaa tgcagatgaa aaaataagac ttgctacatt atcattagga gctataatac
     1021 cagataaatt tatggagttg gcagaaaaaa atgaagtagc atatgcattt tatcctcata
     1081 ctgtgtataa gaaatatgga gtttctcttg atgagataga aatggatgag tggtatgata
     1141 aattggttaa tgatagtgat ataagaaaaa aagaaataaa tcctaggcag atgttgacaa
     1201 aaatagcaca gatgcaacaa gaaagtggat atccatatgt agtgtacata gatacagcta
     1261 atagagaaca tacattaaaa gatgttggaa ttattaaaat gtctaactta tgttgtgaga
     1321 tatttcaata tcaaacacct tcagaaatag aggggtatgg aggaaaaaat gaatggggac
     1381 aagatataag ttgtaaccta ggttctttaa acatagctaa tgtaatggat aataaaacta
     1441 tagaaagtac agttgaaaca gcaataagag cactgtcatt tgtagcagat agcacagata
     1501 taaaaccagt gccaacggta agcaatagta acagcaagtc tcattctata ggattaggag
     1561 ctatgaattt acatggatac ttagtaagag aaaatatttt atatacttca gatgatgcta
     1621 tagatttttc taatgtattt tttgcaatgg ttagatacta ttcaattaaa gcttctatga
     1681 aaatagctat agaaagaaat caaacttttg aaggatttga taaatcagaa tatgttaagg
     1741 gaagaaatag caaggtttta tcaaaatact atgaacaatc ttatctacct aagtcagaaa
     1801 aggtaagagc attatttgaa gggatttata taccaacaaa agaagactgg acaaaattat
     1861 tggatgaggt aaaagaaaaa gggatatata atgcatattt aatggcagta gcaccaactc
     1921 aaagcattag ttatgttcaa aatgctacat caagtataat gccaattact gagccagtag
     1981 aagtaagaac atatggagac tctactacaa tatatccaat gccattttta acaaatgata
     2041 atatgttgta ttatcaatca gcatacagga tggatatgag aaaggtcata gatttagtgg
     2101 caacagttca aaaccatgtt gaccaaggta tatcaacaac actgtttgtt acagatgaaa
     2161 agacaactag agatatagct agacattaca tatatgccta taaaaaaggt ttgaaaagtt
     2221 tatattacac aagaacaaag atgacaagag atactcatga gtgtctagtg tgttcagtat
     2281 agggagagaa atatatgaca tttaatttac ataaaataca taatgcagtt aactggaata
     2341 gagaagaaga tggatttaca caagcttttt gggaacaaaa tgtaaaacaa ttttggcttc
     2401 ctgaagaaat atctgtttct aaagacataa aggtatggaa tgaactaagt aataaagaaa
     2461 aggaattata taaaaaagta ttgggtggtt taacattact ggatacaaaa caggggaata
     2521 atggaatacc atctatgatg agtttaacag aaaacctaca aagaaaagct gtcttgtctt
     2581 ttatggggac tatggaagaa atacatgcaa aaagttattc ttctatattt atgactctac
     2641 tttctaattt agaaatagat gaattatttg aatggataga aacagaacca acattacaaa
     2701 gaaaggctga tttagtttta gctcaatacg aaaatactac aaatcaagag ggattatacc
     2761 tatcaatggt tacaagtgta tttttagaga gtttcttgtt ttattcagga ttcttttatc
     2821 cattgtatct atctggtcaa ggaaagatgg tagctagtgg agaaataata tcgcttattc
     2881 ttagagatga gtctttacat ggtaagtata taggattgct agcacaagaa atatacgatt
     2941 catttgataa aatggataag gagatgttag aagagaagat gtattctata ctttatagcc
     3001 ttatggagaa tgagatagag tatacaaatg ttatatacag agaaagtggg cttgaaaaag
     3061 aggttgtgaa ctttttaaaa tacaatgcaa atagagcttt agaaaatcta gggtttgaga
     3121 agctatatac tgtagatgct ataaatccaa ttgtattaaa tggtcttagc actgaaacca
     3181 aaacacatga tttcttttca actaagggta atggatacca aaaaggagtg tatgaggaat
     3241 tagaggatga agattttata atataattta taattatgac acactgttgt cacttttagc
     3301 gtgataatat caatgtataa aatatatatg gaggtatttt tatgaaatat gaatggagaa
     3361 aaagagataa agagttatat ttacctaaaa agaatcctgt aatagttgaa ataccagaac
     3421 agaaatttat catgttaaaa gggagtggag acccaaattc taaagaattt actgaagcaa
     3481 taggagtact ttattctttg gcatatgcaa taaaaacaat gcctaaaaaa ggaattgtac
     3541 cagaagggta ttttgactat acggtttttc ctttagaagg tatatgggat aagggagaaa
     3601 aaagtaaagg cttagatgta cttattaaag aagatttaat ttacactata atgatacgtc
     3661 agccagattt tgttacacaa gacctgttgg ataaagctac tgatatagta aagaagaaaa
     3721 aacctcataa tcttcttgaa aaagttagat ttgggtctat taatgaaggg ctatgtgttc
     3781 aaatgcttca tataggttca tatgataatg aaccagaaag ctttgacatt atgaacgaat
     3841 tttgcaaaaa aaataaactt ataagaaaat cttacacaca tcgtgaaatt tatataagtg
     3901 atgctagaaa agtatcgcct gaatcattga agacggtttt acgttttgaa gtagaaaggg
     3961 aagtataaat attttgaata atttataaga gttataaaaa gtgaagtctg ttgagacttc
     4021 attttttgat gcaattttaa aattaaattt gatgtttcat aatattttaa aaactttcta
     4081 cttttattag tgtgaataac tgttgctatt aaataataat actaattata taagtattat
     4141 tatataatat ttatatatta ttatataaat agattattag taagatgtat gcattattaa
     4201 ttaaaataaa ttacatatac tatttaatat ttcatttaaa atattaaaaa atgtagataa
     4261 aaattgtttt attaagaaaa aattaatata ttgacaaaat ttaagtgctc atttaaacta
     4321 ttaattgata ataatattca atattaaata ataggaggcg ttatgaataa atatcttcta
     4381 ataaatccag tggcagaaag aatgtatgga gaaaagtttc accttattaa agcaaatttg
     4441 ataaaaaagg ggtacataat agcagaatgt gaaccacagt tagagtatgt aaaaaagcag
     4501 tacaaagagt atacaaaaaa gactaaaaat acaatgttgg attgtagatg tccagaaagt
     4561 ataagtcttt taaaaagaaa taatttgata aatgattttg aagtacctat gatagaacca
     4621 atattagtaa ggacttgtag ggttttatac gataaatata taaaaagtaa agatgatatg
     4681 ttggtagtaa cctgtccatg tacacaatta agagactttg caagtgaaaa gtttaaggat
     4741 aagggtaatg ctatgtttta tacatggaaa gagtttgctg aacaagaagg tgtaaaatca
     4801 cttggaaaaa tagatgaatc accaattcca ttaggatatt tcaaagatac ttttgaaaaa
     4861 gttcttgaag tatcaagtga agacgaaata ttaagtgaaa tacaaaaaat aagaaatggc
     4921 tttgaagata agtatgacat agtagaaatg ttttattgta aagatggttg taataatgga
     4981 gatggtttgt aaatggtcaa taaatttaat aataagtttt taaggctagt aaagagtgtt
     5041 ttgatatttt ttataattct tttattatgg aaaattacaa attatcttgg tatttggagt
     5101 gattatatat taccatcacc tgaaaaggta tatagtacat ttttaaatat gataagtgat
     5161 ggttcaatat ttattaatgt atatgcaagt atgaaaagag ttttgatagg ttttgcaata
     5221 agtactgcta taggtgtgcc tttagggata ttctttggaa tttatagtgg agtatatgaa
     5281 tactttaaat ctctaataaa ttttttaagg aatacacctc cccttgcttt aataccaatg
     5341 cttatattat ggtttggtat aggagaggaa tcaaaaatta taattatagt tttagcatcc
     5401 tttttcccaa tctttacaag cacattgaaa gggattaaaa attgtgactc aaaactgatt
     5461 gaagtgggta gagtatttga gttttcaaag cttcagataa tatttaaaat aataatacca
     5521 aatgccattc ttgatattgc tgttgggtta aagttagctc ttggatatag ctttagagca
     5581 attattgggg cagaacttgt agcagcttca tctggtcttg gatacttgat atctgatgga
     5641 aaagaaatgt ctagaacaga tgttgttatt gtaggtataa tcgtaattgg acttttagga
     5701 ataattacag attacatatt ttcgattatt gtgaagaagg taagtaagga aaagatggtg
     5761 gaagcatatg aataatttag tatgtgactc tggatatata attagaaatt tatataaaag
     5821 ttataaagtt gacaatgaag aacatgtagt tcttagtgat atctctttaa acatagatag
     5881 aaaccatata acagtaattt taggtgaaag tggttgtgga aagactacct tgcttagagc
     5941 aattgcatca cttgaaggta taaatagtgg tgaaattagg tttattaatg ataataaaga
     6001 gtatagacct aatgttggtt ttgtttttca ggagagcaga ttaatgcctt ggttaaacgt
     6061 aagtgaaaat atagcttttc ataatcaaag aaaaaatact attttaaaca aggtacttag
     6121 aaaatttaaa cacaataaaa gaattagcag taaaattata aatgataatt ttagaagctt
     6181 agaaacaaat gataatttga acaaatcaaa taaatataat aggaaaagta atattgatgt
     6241 agaaaaatat cttaagatga tgaacttgca gaaatttaaa aactcttatc caaatgaatt
     6301 gtcaggaggt atggcacaga gagtaagcat agctagagct ctttctttca atccagatat
     6361 gttgcttatg gacgagcctt tttcggcatt agattatttt acaaggattg atatgcaaaa
     6421 tgaagttata agaatacata agtcaactaa taaaggcgtg atatttgtaa ctcatgatat
     6481 tgatgaagcg ttaaagatag gcaagaaaat agttgttttc acagacgaaa gaaaaataaa
     6541 agaatttaat atagaagatg gttataatag agatttaact tcagattact atattaaatt
     6601 aaaaaaagat attttaatga caatgaaatc aaataaaggc atttaaagaa attaataata
     6661 ttttaaggag taaagtaaat gaaattaaaa aagctattga taggtataac gatattaaca
     6721 cttgcaacag catctttagc tggatgtgct aaaaaaaata atggagaaaa actttcagaa
     6781 ataaatttaa cttatgtaaa atcaccatta aatgttcctt ctataattca gaaacaggat
     6841 gatttatttg gaaaggaatt taaaaaggat aatataaaag ttaacttcca cgaaattact
     6901 acaggtccag agcaaacaca agctttagct gctggagaaa tagacttctt acatgcacta
     6961 ggtggtacat cagctttaat agctgcatct aatggagtag agttaaagat attaaacact
     7021 tatagcaggt caccaaaagg atttatgatt ttgactaata ataattcaat aaaatcagca
     7081 gcagatttag taggtaagaa ggtggcaggc ccaaaaggga ctatattaca tcaggtattg
     7141 atttcagcac ttgataaaga aggattaagt atggatgatg tggaatttgt taatatgggt
     7201 attccagagg catcagcagc tctttctgat ggcagcgtag atgctgcatt gattgctggt
     7261 ccagcagctc ttaaagctat gaagtcaggt agtaaacttg tagcaaatgg tgaaggttta
     7321 gtagatggta taatagtaac tgctgttagc acagactttg cagagaaaca tccagaaata
     7381 gttgaaagat ttatgaaggt tgaaaaggaa actttagaat atgtaaataa taattttgat
     7441 gaagctatgg aaaaagtagc taaagaagtt gatttaagtc ttgaagaaac taaagaatta
     7501 tatgcttggt atgattttag tttagatata acagataaag acatatcttc gcttgaagat
     7561 acacaagact tcttaataaa aaataagtta caagagaaaa aagtgaacat aaaagaatta
     7621 atctataatg ctaagtagaa actatgataa aataatacta agtaagaaat ataataaaag
     7681 tttaagttac cttattaagg taactttttc tattttgaat tagtaatttc aatttatttt
     7741 agaatgatta gttgataata atgattaatt aatcataaaa agatactttt ttatagagtt
     7801 ttattaagat gtctaataat atggttatac ttatgaagtc agtaattgtg aagttagtaa
     7861 tatgattaaa tttatatatt gtaaaaagat tataatatat taaaattgga ctgttatatg
     7921 atgagtaaat aggtatatta ggaggaatta tatgagttat aaagtatata ttgtggaaga
     7981 tgatatatca ataagtacat tacttaaaga ttatattgag aaatatggat ttgaagtgat
     8041 tgttgaagaa aactttgatg atataatgat tacatttgaa agttttaagc cacacttagt
     8101 tctattagat gtaaatttac ctaaatttga tggtttctac tggtgtagat gtattaggca
     8161 acagtcgaat ttacctataa tttttatatc agctagagat ggtaatgtag accaaattag
     8221 agctttagaa agtggagcag atgattatat aacaaaacct ttttactatg atgtagtaat
     8281 ggcaaaaata aagagtcata taagaagggt atatggtgaa tattcaccta aaatagacga
     8341 aagaatagtg gagctagatg gattaaagtt ttatccagag aggttagaac tagaattaaa
     8401 tggtgagaat ctaattataa ctaaaaaaga aggtatactg attgaatgtc ttatcaagag
     8461 atacccaaaa gtagtaagta gagattatct tttagaaaag atatgggatg atattgagtt
     8521 tgtagaggaa aatactttaa atgtaaatgt tagcaggata aggaaaagat ttgaggaatt
     8581 aggtatagat aatgctgttc agactgtccg agggttagga tataaattaa ataagaattg
     8641 gtaaggagaa agtatgaagt tatttttaaa agattataaa ggttatattt ttatatatta
     8701 tataggcata gttatgacaa ttatttactg taatcttatg aagtttatta gttttggaga
     8761 gagtttatat attcttttat ttagttcttt tatattgtta tgttttttaa tatataagta
     8821 ttataaaaat agagaagtat ataatctgtt tgaaaaagga gtacattctt tggaggagtc
     8881 atttttgtat ttaggaagct cattcttggg gagaaatata tcagttattt taaaacaaca
     8941 acataaattg tatcaatcaa tgatacaaga gcataaaaag atacatagtg aacatttaac
     9001 tttcatcaac caatggattc atcagatgaa aacaccaata tcagttataa gccttcagct
     9061 tcaagattat gagggagaag aagtaactga aaatataaga aaagaaatag atagattaaa
     9121 taaagggtta gatatggcta tgcattttgc aagggcagat aattttcaga aagattttat
     9181 agttgaaaaa gccaaattaa atgaaattgt atctagtatt gtaaatgaag aaaaaaggtt
     9241 atttataaaa agtggaatga ttcctaagat tcaagtagac aaatttactt atgtttatac
     9301 agatgtaaaa tggatgaaat ttataatagg tcaattaatt acaaatggag tgaaatattc
     9361 taaagattat agtaactacc taactataaa atctgaagaa aataataaat atgtaatttt
     9421 aagtataata gatgaaggaa ttggaattgt ttctaaagat ttaaaaaggg tatttgaccc
     9481 attttttaca ggagaaaatg gtcgtaagtt tggagaatct acaggtatgg gtctttatct
     9541 agtaaaaaag gtatgtgatg atttaggtca taaagtaagt ataaaatcag aagtaggaaa
     9601 aggtacagaa gtttcaatct actttaataa agatgtaaat agtttaataa aggtgtaaat
     9661 tatctgttac aatagggttt ttagttaatt gtactttttt attatacaat ttattaaatg
     9721 aaatattcat agttaaataa tcaaaattta agagagttta tttgcaaacc ttacatagat
     9781 gtaaggtttg tgtaatgttt gcagatagtt aaattttcta atctaaatta caattaatat
     9841 aagaaataac ataagagaaa taacataaaa aaagaaattt tgtaaaaaag gaaggatgtt
     9901 tattgatgag tttagatgtt atttttatat tgaaatcagt cataatagct atagtagagg
     9961 ggcttacaga atttattcca gtttcatcta caggtcatat gattttggtt ggtaatttaa
    10021 tagattttaa ggggcaattt gctgaaatgt ttgaggttgt aatacaactt ggggctatat
    10081 tagcagtagt agtactttat tggaaaaaga taaaagatag ggttatagaa ttttttaaat
    10141 ttatatttac tggtggaaaa gaaggaaaga ttggttttag atttggaatg aatgtaatta
    10201 taggttgtat accatttgcg attatagggg ttctatttta cgataatata aaattattgt
    10261 ttaatttaca atctgtaatt ataggattta tagtgggtgg gattttatta ttagtagtag
    10321 aaactttgtt tagaaaaaag aatcattcca cagataatat agataaaatt actccaatac
    10381 aagctttaaa ggttgggaca ttacaagttt tgtctgcatg gccaggtatg tcaaggagtg
    10441 catctactat tatgggtgga tggatagcag gacttaattc tcctacagca gctgagtttt
    10501 ctttcttttt agcagtacca gctatggtgg cttcttcagg aaaagattta tttgaatttg
    10561 attattcaat aatgacacca acattgtgga ttgctttggt agttgggttt atagtagctt
    10621 ttattgtatc aataatagtt atggaaaaat tcgtgaattt tttaaagaaa aaaccaatga
    10681 gagtgtttgc tgtatacaga ataatcatgg gtgtagtact tgcagtatta gcttttacta
    10741 acataattag tgtatagaaa aaatagattt attatctata aaaattaaac taagataaat
    10801 tataattaac aattatatat atgttatttc aaaaattaaa ttaggaagga tagttaaata
    10861 atgaaaattt taactgtaaa taatttaagt aaagtatatg gtaaaaaaat aattttcaac
    10921 gcattaaatg acataaattt tagtatagaa gatggagaat ttgtaggaat tatgggtcca
    10981 tctggtagtg gaaaaactac tcttttaaat atgatttcta ctattgacaa gccaactaca
    11041 ggaactatgg aattaaaggg caagaatcct ttattgttaa gaggtgagga acttgcatta
    11101 cttagaagaa gagagttagg ttttgtattt caggatttta atttattgga tacattaact
    11161 ataggagaaa atatagtatt gccattgaca ttagataagg tcagtgtaaa agagcaagat
    11221 gagagattga atgaagtatc tacaatatta ggtataaaag atttattagg aaaaagaact
    11281 tttgaagtat caggtggtca agctcagagg actgctattg ctagagcatt gattaacaat
    11341 ccatcaatac ttttagcaga tgaaccaaca ggtaatttag attcaaagtc atcaaaggtt
    11401 gtaatggagc tatttcaaaa gataaataaa gaaaataaag ttactactat gatggtaact
    11461 catgacccat tagcagcgag ctactgtagt agaatcttat tcataaaaga tgggtctata
    11521 tataatgaaa tatataaggg tagtagtaga gagcagtttt atcaagagat aatggatgta
    11581 cttacattat tgggaggcga taactaatga atataaaaca gtttgctatt aacaatattt
    11641 caagaaatat taaagcgtat ttaggatact tagcaagtat tgttatatca tcttcactgc
    11701 tattttcttt tattatgttt acaagtcatc cagatttaga tgtatctcaa tttcctgatt
    11761 atttaaaaga aggtctcaag atgagtaaga taattgcata tctattctta ttcttctttg
    11821 tgttttattc agttagtgtg tttcttaaaa gtagatataa agaatttgga atactttata
    11881 ttacaggtat atcaaagcgc caagtaatga aattaatctt tatagaaaat atgataataa
    11941 atatagtatc ttctgtaatt ggaataataa taggcttaat ctttgcaaag atatttttag
    12001 tgattatgtc tactttttta gaattatcac cattaaaatt ctatatacca tttaatgcga
    12061 tattgtctac tttaatctat tttatgattt tagctgtatt aacatcaata tttacttctt
    12121 ttatagttaa agaaaatcag gtgttgaagt tacttaaagg tacaaaaact ccaaaaccag
    12181 agcctaaaac atcactaatg cttgctatat taagtatttg tttgtttata atagcgtatt
    12241 ataatgctat tacctcaacc gaccaatata caacatatct gcgcttagtt ccagttacat
    12301 cccttactat agtagcaact tacttgttct tttctcaatt tagtgtattt tttatgaaga
    12361 agctaaaact aaacaagaga ttttatagaa aaaatacaaa tatgctttgg atttcgaact
    12421 tgatttataa ggtaaaggat aatgctagaa tatttttctt gataactata acttctgcag
    12481 tagcatttac tgctataggt acagtttatt ccttttggaa agatgtagag cgtcaaatta
    12541 atttaatata tcctaacaca atatattatt caacaatgac attgcataat gaccctaaaa
    12601 agcctgatag caaagaaaaa gacaaggaaa gaatgatgtt tatagaaaat aatctaaaaa
    12661 aagagaatgt agactataca aggactaatg gtgaatttaa aactgtattt cccaagaaaa
    12721 gtgattttaa tgtcagaatt atgaaagaat ctaagtactt agaaattaca gaaactatag
    12781 gggttaagtc tataagtttt gaagataatg aatgtatatc attattatca tcaagtttac
    12841 caggaaataa taacgtcaaa gaaaatatta ttgtagggaa taggggttta aaggtggcaa
    12901 aacaagtgga agaatgtgtt atgccagcat attacaaaaa tatgtatgtg gtaaaggata
    12961 atttttatga tagtatagaa tcaaaattta tagtggatag atttactgca tttgaagtag
    13021 aggattcaag taaaactata gagatatgca gagaatttga aaataaattt aataatgagt
    13081 cagggatgca accatatttg tttttctcta aagaattaat gataggaact gggaaattaa
    13141 tgtattctac atttatattt ttagcaatat ttataggttt aattttcttt gttactacaa
    13201 gtagtttttt atataataaa ttttatatgg attgccaagt tgataagaaa aagtatgaac
    13261 agttaaataa attggggata acatataatg agattaaaaa agcttcaaca attgagattg
    13321 gaatagtatt tttgtttcct tatgtagtgg ctgttataca ttcaatattt gcacttactg
    13381 cactgaaaaa ttcttttgat atagaagtag catcatcagc tgttttggta atgggaagct
    13441 tgttcttggt tcaaatagtt tattttttaa ttgtaagaaa taattattta aaagagataa
    13501 ggttaaattt agtcaatttt tagttactat tattaaatga taataaaaag ggtttaaaat
    13561 taattaatat tactggaatt atagatgttt attttttcag taatattttt tattgtatta
    13621 actattaaaa ttttgtacaa atataaaata taatatggta aatgtcaaaa ttataaaaaa
    13681 attctactta tataaaatat aaagaggtta tatgtacata tgtgtagaaa taaataataa
    13741 aaatatcaat taatgttatt agggggggtt actcattagt aaattagcta atacacttag
    13801 aatgatgata ttaattcaaa gtagaggcaa aatgaaatgc aaagaattat cagaagagtt
    13861 ggaagtaagt gaaaggcaaa taaaaagtta taagatgtat ttagagcaag ctggtatatt
    13921 tataaattca acacctggga tttatggtgg ttatgaaata gataaatgta atagcatctc
    13981 attaattaaa ttattagata gtgaagtttc aattctagat atgataaatt cacagttaga
    14041 gtataataat gatatttaca aaaatgaatt taataatatt gtagaaaaaa taaaagcagt
    14101 attaaataca ggagaaaaaa gcgatacata tatggattat tttacagttc aagcacaacg
    14161 taattgtgat tatgagtctg aaaaaaataa gtgtaatgaa ataattagag catataccac
    14221 aaaacacaaa ttttggatag aatattattc attaaattca ggaaatagtg aaagaattgt
    14281 tcatccatat ggtttattta attataaatc agatacttat atggtagctt tttgtgaaaa
    14341 aagatttaag tttatagact ttaaattatg tagaattaaa gattataagg ttttagaaga
    14401 aaaatataat gtagataaaa gttttagttg ggatgaatat agtaaaaata gtataggaat
    14461 ttataaggga gaagaaatta gtgttgttat aaaaattagt catccatttt caacaattat
    14521 aaaggaaaaa gtttgggtta ataatcaaca aatcatagaa tatgatgata aatctataat
    14581 gtttaaagct aaaatgagag gttatgagga gataaaaagt tggattttaa gtatgggagc
    14641 ttatgttgag gttgtagaac cagatagact tagaaatgat atattgtcag agattgaaaa
    14701 aatgaaaaaa atttactgat tttttcaaat aatttatact tggaccaata tacttcacag
    14761 tctagtgtta atatattaat tgagagaggt gatattatga aaatgaaaat tgaatttagt
    14821 acaggttgta taccaatatc ttataattcg ctctttatga gtattataaa agaagcaata
    14881 agaaaatcta atgaagatta ctataaaagc atgtactatt ataaagaaaa gaataataaa
    14941 aaaactaaaa attttacatt ttcggtttat ataaaaaagt atagtattga aggtgacaat
    15001 tttattatag aagataaagt aatattaaat ataagtactc cagatttaga attgggtttt
    15061 catatataca atgggttaat gacttctaaa aaatgtttat ataaagacta cgaacttaca
    15121 agaattagaa tcgatttatc aagagaaaag aaggtaacag aagaaagggt actatttaat
    15181 gcattgtctc caatctgtgt aaagtctaaa gaaggaaaat tcttggagat aactgatgat
    15241 agatacatag aagaactaaa ctatataact aatgaagtgg ttaaaaatta tagggggaat
    15301 ggattaaaaa gaaagttaga atttgaaaat ataggtctca aaaaggtagt agttaaagag
    15361 agcttaagag aatttaaaaa aataacaggt aaagagtatc aatatattaa tggttacaaa
    15421 ggtaaattta tattgaaagg tgatattgat gatttaaatc ttatctacaa tctaggaatt
    15481 ggttttagac gtgctcaggg atttggagat gtagatattt tagaatggag gtgagataaa
    15541 tgaagttaag agtttataga ggagattggt tttttaatat gggtatagtt ggatttttaa
    15601 atataataaa gaaagcagat aaacaagctg aaatatttat tatggaagat tatatcgaat
    15661 ttgatagctt atttcttgaa aactttcatg agtattattt taattacttt atggatgaat
    15721 atgatgtatc aaaaagaatt aaaaaaaata ttgattatag tataaatttt attaaatcta
    15781 agcctgatag aatcaaagat ggaattaaga aaattaaaga tagtgtgaaa caacaaaatg
    15841 ataagattaa aaaatttgat gaagaaaaat ttaacctgat aaaagagaag ctagatagta
    15901 tgtctaagat aaaaagttat gatgaatttg acagtttaga agctttagta gatgagacta
    15961 tagatatatt taaaataaaa tcaataaatg acaggcttac agcaaatctt tataaatatg
    16021 tagttcaaga taattatttt ggacaagtta gtttttttaa tgttgctaag gcgaaattag
    16081 atttagatgg attaaaacag gtaatgttta atgattattt aagacaaatt atatattttg
    16141 gagagttaga agatttatta gaagagaatg actatgataa gttgaaaaac tatttaaatg
    16201 atagattaaa tagtatagct aaggatataa atgaaaaaaa agttagtaag tctagtataa
    16261 atactataga aaaaattatg aaggaaataa ataaaaactt tataaagaaa aataaaagca
    16321 tagaagatat aaaaaagtat atggatagtt tagaagtatg tgaaatgtgt ggtttatata
    16381 agggaatatt agatgaatac tcagaaagta attttgctcc acttggagta agcaccaata
    16441 atgctaggaa tatgttttgg aatcaggcgt atgtaccatc aatatgtgat atttgtaagc
    16501 taatattgtt ctgtactcca gcaggggcta cttatacaag aaagaattat ttaattaatg
    16561 aagaaaatga gttttattta tttgttaaca tggatacttg tataaatgag ctttttgaaa
    16621 gaaataatag tttaaaagct caaaaaagtg agtattcaga ttccaaagat gaaaatcctt
    16681 ttaatcagct tataaaaagt atagtagaag aaaatacatt aaaaagtgaa tggcaacttc
    16741 gtaatatact ttttgtggaa tttaaagcaa gtatagattc taaaaaatgt aaaataaatt
    16801 attttaatat tccaacttat ttagcaaaat tttttacaga caagtatgca aataaaaaaa
    16861 tacaaagtat ctatgattac aagcttaaat caaatgtact agatttactc ttgaaaaata
    16921 gggacttgaa gcatctaact aataacattt taagaaataa agttaaaaat gatatggaaa
    16981 gcaacaacaa atcaaatata tcaggaatag attgttttag agtagtccaa ttaagagtat
    17041 taataaatag ctacaaaaag ggggtatata aaatggatag taaaaatttg gagaagaatg
    17101 atgaaaagct gaaaataatt tattatttag gttgtgatat acatgactat tttgttaata
    17161 aaaattctaa aaataaaata gatggagtaa gctataaact tttaaattca gttaaagtcg
    17221 gaaataaaag tgattttatg gatacaatta taagagtttt tatgtcagct gaaaaacaga
    17281 ttccagcatt tattttagat atagaaattg aaaaagattt agactttgaa tctataggtc
    17341 atgcttttat atctggatta atttctggaa aatatgaagg aaaagataaa ctaccaaacg
    17401 aaaaggagga aaaataagat gaaaagagga ttaacattta cagtaatagc acaggctcaa
    17461 agtttaaact atggtgaagg gataggcaat atatcagaat taaaaaaatt aagtagagct
    17521 gatggaaata tgtatacatt tgcatctcgc cagtgtttaa gatatgacat agtacgatta
    17581 gcagcagaat tgtttaattg gaatttacag gttgtcgata aagaaaaagg tactattcaa
    17641 tttaaagata atataagtat aagagaatca gaagaaatgg atttatttgg atatatgaaa
    17701 acaaataaaa aagatgagaa agataaaaaa ggaggttctt tgactagaga agctactgta
    17761 agattaagta atgcgatttc tcttgagcca tatagaagtg atatggattt tttaaataat
    17821 aaaggatttg cagatagaat aggagaacac cctaatcttg ccaacataga gcaacattta
    17881 agttactata catatacggt tactatagat ttatctaaaa taggtaagga tggagatata
    17941 gaacttgata ataaagagaa atgtagaaga gtagtagaat ttttagaaat aataaaagta
    18001 ttgaatagaa atattagagg tagacaagaa aacttatctc ctctatttgt agtaggagga
    18061 gtatatgaaa tagcaaatcc atttttcttg ggtagaatta aattaaaagg agataaaaat
    18121 ggatttaaaa taaataagca agctatagaa gaagtaattc aaggtacttt tttaggaaaa
    18181 gatttaaaag aatttaccta tgttggaatg gtagatgggg tatttataaa taaagaagaa
    18241 tttaagggat tgtttgaaga taatttttta agtgtagata aattttttgg acaactagtt
    18301 aaagaagtaa aggaatatta tggggtgaat taatatgaag gttttgaaat tagatttatt
    18361 tcaagaaact gcatgctata aaaaaccatt tgctatgaag attacagaga catatccact
    18421 gcctccatat tctacaataa atggattaat tcacaaagta ttaaatgcta cagaatatat
    18481 accgtttaat ataagtgtac agggaacata tgaaagtata tttaataatt accagacaac
    18541 atatttttat aaaaaagata gtattacatc tatgccaatg aatagtcata tgctattgaa
    18601 tgtaaatttg ataattcata taggtgggga ttttgattta ttggaagaaa tatatgatag
    18661 tttattaaat tatgacgagc atttatcatt aggtagaaaa gaagatttag taagaattaa
    18721 tgatattaga tttgtagaag tcaataaatt tgaagtagat gaggatatac aattagatga
    18781 ttatgaaaat tataagttat cagaatgtaa tataagaatg cctatatata taccaaaaac
    18841 aagtttacaa gatacagata tagatggtat aagttatagg ctcaataatt actatgataa
    18901 taatgctgtg gaagataaaa aaagggtttg gaataaggta gatagttatt atgttgatga
    18961 tggaaatact atatcaattg gagatatatt attagataat gaaggagatt tattatattt
    19021 taataatata aattaggagg gagagtatgt tatatgctaa atctaatcca gtggaaacac
    19081 ttagagagca tactgatgaa cttttaaaac aaatgaatgt attaagagaa agttatggaa
    19141 aaaatataaa tagcttagat tttctagaag aggagatatt ttggcaatta cttgactttg
    19201 ttatagaatt tcatgatatt ggaaaggcat tttcaccgtt tcaagagtta ataaaaagca
    19261 gaatggatac taaaataaat gaacctaaaa tagtaactca tttagaaaat aatgtaggcc
    19321 ataattatct ttctcctgca tttattgatt atagttatat agacaggaaa aaaaataaag
    19381 aacttagagc agtattaaat caagttattg tatatcatca tgaaagagat atctttatag
    19441 ataaagattt taaaaattta atacaaaaaa tattagatga agatttaata aataaagttt
    19501 atgaactaca gcatgaattt aaagttagat atccaataaa aacagaaaga ttgagtaaag
    19561 tatatctaca aagtgtagaa aaaagaatag ataaaaatca taagtactat aatttatata
    19621 ttatgttaaa aggaatacta catagactag accatagtgc ttctgctcat gaagttgtgg
    19681 aatgcaataa tgttataaat ataggagaac agacagaaaa ttatttaatg aaagagtttg
    19741 gtagtttgag agaggctcaa tcatttgcta aatctaatag aaataagaat ataatactga
    19801 ttgcatctac aggaatggga aaaacagaaa ccgccttaat ttggatagat aaagacaagg
    19861 cattttttac acttccatta agagtaagta taaatgcatt atttgataga gcaaaaaata
    19921 tagttggagt tggagaatca aatgatacat tcttaggttt gttacattca acagcaatag
    19981 attatctaga agaaagtaat caagaaaatt caagtgagat tgtagattta gcaaagttat
    20041 tatcttgtaa actgacattt tccacaatag accaaatatt taaatttcct tttttgtata
    20101 gaggctatga aaaagtgtac tcaacattag catattcaaa agtagtaata gatgagatac
    20161 aagcatattc accagaaata gcagctgtat tggtaaaagg tatagaaatg atacataaga
    20221 taggtggcag atttatgata atgacagcta ctatgccaac catatatata gatgaattaa
    20281 aaaaaagagg tgtaatgaat agtaacttag ctgacttgac atgtaacaca gaaaaaataa
    20341 gacactgtgt gtctattgta gaaaattcaa tagatgaaaa cttaggtaaa ataatacaaa
    20401 gtggaatgaa taaaaaagtt cttgtaatag taaatacagt taaaagtgct gtagaaaaat
    20461 atgagttaat agaagaaata gttaaaccaa aaggaataga tgtcaactta aatcttttac
    20521 attctatgta tatacaagaa gatagagcaa aattagaaaa atatataaaa gaatttgcag
    20581 atagtgatag taatggtatt tggattacta cacaattagt ggaagcatcc cttgatattg
    20641 attttgatga gcttcataca gaaaattcaa cacttgacag tctatttcaa cgatttggta
    20701 gatgttatag aagtcgtgag tatgaagaaa atagcccaaa tatatttata tatacagaaa
    20761 aagctacagg aataggtagt atttatgata aggatattgt agaaaaagga cttgagctac
    20821 tgaaaacttt tataaatgga aaagaatgtg tgaagatgaa agaaaaatat aaggtagaaa
    20881 tggttaagat actatactct aaagaaagtc tagaaggtac agcttttgaa aagagattta
    20941 ccagtgcttt aaatatttta gatactataa cgccatatag tttaggttct aaagatgcac
    21001 aaaatatact tagaaatatt gaaggatata cagtaattcc agaggatata tataataaaa
    21061 ttgaagaaac tttaatcaaa gattatgaag agttgggaca agagcttata aatgcatata
    21121 gtaatgatga tagacaattg ataaataaaa taaagtctaa gagaaaaaaa gcaagaagag
    21181 agataattaa aaaaactgta aatttaccaa tatataaagc taaacaaaat gtaattgaca
    21241 tacaggtgaa aggtcttgaa gatttaaaaa tacttttata taaatatgat atacacgaaa
    21301 ataagcatac aaaacagata tttggtaaag gtgtgcttat aagtaatgaa atagaccaat
    21361 ttatttgatt tttatattaa caatgtggct aaaatttact aaagaaggtg attttgtgcc
    21421 aatagattta gaagtattaa aagttcaagg tgtaaaaata aattattatt atgtatgtaa
    21481 aagaaaatta tggctatttt ctaaaggaat aaccatggaa caaaatagta gtagagtatt
    21541 gtctgggaag atagtacatg aagattcata taaaagaatg aaaaataaag aaatgttgat
    21601 tgatgatatc ttgaagctag atataataaa ggacgagtat gtaaaagaag taaaaataag
    21661 ttcaaagatg aaagaagcag atgaaatgca gttatactat tacttatact atttaaagaa
    21721 tagtatgggt atagagaaaa agggaactat aaattatgtt aaggaaaaga aacaagaaga
    21781 attgatattg acagaagaaa aagaaagaat tattgaaaac acacttataa atataaatga
    21841 aataaatatg cagttatctc ctcccaaaat tgagaaaaaa cattattgta caaaatgtgc
    21901 ttattatgaa ttttgttttg tgaaggagga gtaacaatgt ataaagacta ttatattata
    21961 agtaatgggg atataaaaag gaaagaaaat acgatttact attacaattt agaaggtgag
    22021 aaaaaaatat taccagtaga acaagttgat gatattcata tatatggaga agtagattta
    22081 aatacaaaac ttataaatta cataagtaaa tatggggtga tgcttcattt ctacaattac
    22141 tatggatatt attcaggtac atattatcca aggaagaaaa atgtatcagg attcagttta
    22201 gtatgtcaat cagcctgcta tttagataaa aatgaaagat tatacttagc aaaatccttt
    22261 gtggaaagtg catcatacca tattttaaga aatatgagat attacaaagt tgatgaaagt
    22321 ttaattgata ctataaagga tgaactaaat gatttgaatg atgtcaaaga aatatcagaa
    22381 cttatgggta aagaaggcag aataagaaaa acatactata aatcatttaa tcaaatatta
    22441 aaagatgatt ttgaatttta taaaagagaa aaaagacctc ccaaagaccc agtaaatgct
    22501 cttatgtctt ttggaaatag cataatgtat acgaatgttt taagtgagat atataagaca
    22561 caacttgacc ctacaataag ctttttacat gaaccatcaa caaagagatt ttctttaagt
    22621 ttagatattg cagaaatttt taagccaatt atagttgaca caataatttt tagcctgatt
    22681 aataataaaa gaattaataa gaatgatttt gaatatcaag ataaaatttg ttatttaagt
    22741 gaaaaaggaa agaagaaatt tttgcaagaa tttgaaaata agatgcagac aactataaaa
    22801 catagaaatc taaatagaaa agtttcatat agaatgttta taaggttaga gtgttacaaa
    22861 ttaattaaac attttataaa tgatgaaaga tataaagttt taaaggtgtg gtggtaagta
    22921 tgtttgttat tgttacttat gatattgttg aagcaaggtc gttaaataga attagaaaga
    22981 tacttagaaa atatttgact tggacgcaaa attctgtttt tgaaggcaat attactgatg
    23041 gaaagttaca taaatgtatt tctgaaatag aaaatattat tgataatagc gaggattcaa
    23101 tctatgttta tgagataaaa aatcctaatt caattaaaaa gaaatgttat gggattgata
    23161 agtattctga tgaaatgttt atataggttt gcagtgagcg atatttatgc taaaataggt
    23221 gttaacagtt ggaatataag ggattgaagg tgtatgataa ctgttatcaa ttgcactact
    23281 gctcgctcac tgcaaatttt gatgttttta ttgaattata attgcttgat tgaagtattt
    23341 tcaatgtatt caattatacc tattttgggg ttttatatta actaagtggt atgtaaataa
    23401 gtttgaactg ctatcgcttc aacttttcct tgtaggtttt atattaacta agtggtatgt
    23461 aaataaaaca aaaggagtgg taaaaatggc taagaaaaaa ggttttatat taactaagtg
    23521 gtatgtaaat aaagtataaa caaatagttt tatctaaaaa ttataagttt tatattaact
    23581 aagtggtatg taaatttatt atgtattaca gctgtttcat tattcttaag atgttttata
    23641 ttaactaagt ggtatgtaaa ttaagtaatt tcttcatcaa gtaaattatc atggtagcgt
    23701 tttatattaa ctaagtggta tgtaaatgtt gatgtctctc tagtaccttc cacagtttct
    23761 aaagttttat attaactaag tggtatgtaa ataaagaacc tctttttctg tttcatcaac
    23821 tgtgtttaat gttttatatt aactaagtgg tatgtaaatg catatatata tatcattaaa
    23881 ataagtttta aagtcgtttt atattaacta agtggtatgt aaatataagt aaaacgggtc
    23941 cgagcgctgc tgctaatcct ggttttatat taactaagtg gtatgtaaat aggataaaga
    24001 aaagactcac acaaggcaca gtgtcaggtt ttatatcaac taagggtaga taagtagaat
    24061 attagcaata tcaacgagta tttagaaact tattttatat taataaagca gtatataaaa
    24121 ttgtagaatc aacaatagca tatactaaaa catccacctt atattaacta aatcatatct
    24181 aaataaaata cacttaccta taaacattat aaaatcaata caaaaatgag gtgaaataaa
    24241 atttatgata aagaaattaa acaataaaga cataaataaa atcatggaaa tatgggaaaa
    24301 aagtacaatc aaagcacatg actttataag taaagaatac tggcaaaata actataatac
    24361 tgttaaaaac gaatatatac ctatatcaga tacatttgta tatgatgatg gagatgaaat
    24421 aaaaggattt ataagcataa tagataaaag ctttatagga gctttattta taaagcccaa
    24481 ataccaaaat ctaggtatcg gaggtaaact tttagattat gcaactaaaa aatataaaag
    24541 tctaagctta gcagtatata aagataataa aaaagcagtt gtgttttata ataaaaaagg
    24601 ttttaatata gtaaa
//
LOCUS       DAEMIK010000051        24432 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEMIK010000051 DAEMIK010000000
VERSION     DAEMIK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.051"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            50..166
                     /gene="rrf"
                     /locus_tag="KPK87_001719"
     rRNA            50..166
                     /gene="rrf"
                     /locus_tag="KPK87_001719"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            286..477
                     /locus_tag="KPK87_001720"
     CDS             286..477
                     /locus_tag="KPK87_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG2537872.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            632..2041
                     /locus_tag="KPK87_001721"
     CDS             632..2041
                     /locus_tag="KPK87_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2537873.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVDDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2172..2861
                     /locus_tag="KPK87_001722"
     CDS             2172..2861
                     /locus_tag="KPK87_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG2537874.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKSMER"
     gene            2864..3799
                     /locus_tag="KPK87_001723"
     CDS             2864..3799
                     /locus_tag="KPK87_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG2537875.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3796..4689
                     /locus_tag="KPK87_001724"
     CDS             3796..4689
                     /locus_tag="KPK87_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG2537876.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4767..5513
                     /locus_tag="KPK87_001725"
     CDS             4767..5513
                     /locus_tag="KPK87_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG2537877.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5514..6347
                     /gene="rsmI"
                     /locus_tag="KPK87_001726"
     CDS             5514..6347
                     /gene="rsmI"
                     /locus_tag="KPK87_001726"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG2537878.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6370..7074
                     /locus_tag="KPK87_001727"
     CDS             6370..7074
                     /locus_tag="KPK87_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG2537879.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGINLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7262..7690)
                     /locus_tag="KPK87_001728"
     CDS             complement(7262..7690)
                     /locus_tag="KPK87_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG2537880.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7727..9157)
                     /locus_tag="KPK87_001729"
     CDS             complement(7727..9157)
                     /locus_tag="KPK87_001729"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG2537881.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9289..9873
                     /locus_tag="KPK87_001730"
     CDS             9289..9873
                     /locus_tag="KPK87_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG2537882.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9889..10410
                     /locus_tag="KPK87_001731"
     CDS             9889..10410
                     /locus_tag="KPK87_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG2537883.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10476..10722
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10806..12743
                     /gene="metG"
                     /locus_tag="KPK87_001732"
     CDS             10806..12743
                     /gene="metG"
                     /locus_tag="KPK87_001732"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG2537884.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12773..13543
                     /locus_tag="KPK87_001733"
     CDS             12773..13543
                     /locus_tag="KPK87_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG2537885.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13880..14311
                     /gene="phnG"
                     /locus_tag="KPK87_001734"
     CDS             13880..14311
                     /gene="phnG"
                     /locus_tag="KPK87_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG2537886.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14326..14901
                     /gene="phnH"
                     /locus_tag="KPK87_001735"
     CDS             14326..14901
                     /gene="phnH"
                     /locus_tag="KPK87_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG2537887.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14904..15989
                     /locus_tag="KPK87_001736"
     CDS             14904..15989
                     /locus_tag="KPK87_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG2537888.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16009..16878
                     /locus_tag="KPK87_001737"
     CDS             16009..16878
                     /locus_tag="KPK87_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG2537889.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGNDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTSEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16871..17737
                     /locus_tag="KPK87_001738"
     CDS             16871..17737
                     /locus_tag="KPK87_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2537890.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17778..18491
                     /locus_tag="KPK87_001739"
     CDS             17778..18491
                     /locus_tag="KPK87_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2537891.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18492..19661
                     /locus_tag="KPK87_001740"
     CDS             18492..19661
                     /locus_tag="KPK87_001740"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG2537892.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19665..20501
                     /locus_tag="KPK87_001741"
     CDS             19665..20501
                     /locus_tag="KPK87_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG2537893.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20502..21497
                     /locus_tag="KPK87_001742"
     CDS             20502..21497
                     /locus_tag="KPK87_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2537894.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21515..23203
                     /locus_tag="KPK87_001743"
     CDS             21515..23203
                     /locus_tag="KPK87_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2537895.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMKHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23215..24282
                     /locus_tag="KPK87_001744"
     CDS             23215..24282
                     /locus_tag="KPK87_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2537896.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLELYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9743 a         2745 c         4366 g         7578 t
ORIGIN      
        1 ttctataaac ttattaaaaa cttaatagaa atattaagca taaagattat gtggttacaa
       61 tagcagagag gatacacctg ttcccattcc gaacacagaa gttaagctct ctagcgctga
      121 tggtacttgg tgggaaactg cctgggagag taggacgtag ccacgtgatt ttttttattt
      181 tttgtcaaaa aaaacaaaaa atacgtattt cttttttcat ttcgggaaaa ctaaacacag
      241 gaattatgga ctttataaaa atttaattct tgaggagtga tataagtgaa aaaagttgct
      301 ttaattttag ctataatagg tgcgcttaac tggggtgcaa ttggtattct aggtacagac
      361 ttaataggta gtatctttgg tggaacatat gaaatggtaa gtagaataat atacttcatt
      421 gtaggtttag ctggattata cttaatacca agtttaatgt ctgatgatag agaataattt
      481 atgcttttat gttaaatacc tcattctaaa tgtttagaat gaggtattta attttataaa
      541 aatattaatt tgtttctttt ttaatggtaa aatagataag aattgaagtt ttaaaaagaa
      601 gtattatgta aaaaaggagt actattaata tatgtttatt attaatgaat taaataagat
      661 tgtaaatgaa gaagtaatat cttttcatat gcctggtcat aaaaagggac aggtttataa
      721 aaagttagga tataaagata tactaaaaaa tttgtataaa atggatacaa ctgagatatt
      781 aggaacagat aatttacact ttccagaagg aattataaaa aattcacaaa aaaaagcatc
      841 aagagtattt aaaagtgatt atacttgcta tttagtaaat ggtagtagct gtggaataca
      901 atctgcaata atgtcagtat gtagtcctaa aagtaaaata ataacaaata gagactgtca
      961 tcagtctgtt ataaatggat gtattttagg agatatagat atagagtatg tgccttgtga
     1021 gataagtgaa gagacaaatg tcttaaaagg gatatctata agtaatgtga ttgatataat
     1081 agataaaaac ttggatgcaa aagcagtact gcttacttat cctacatatt atggaatgac
     1141 atatgattta gaatacatat gtaattatgc acatagcaaa aagatggtag tgatagtaga
     1201 tgaagcacat ggtgcgcatt tagggcttag cgagaggctt cctaaaacag ctcttgagca
     1261 aggcgcagat atagttgtac agagtacaca taaaactctt ccagcattta cacaatcgtc
     1321 aatgatacat atacagggag accgagttga tatagataaa atattgaaca tgttaagaat
     1381 tacagaatct tcaagccctt catatttatt attatcttct ctggaattag cagtagatat
     1441 atatgaaaat aaaggaaaag aattaatgga agagctttta aataatatta taactttcaa
     1501 aaataaagtt gattcaaata ttaatatata cgacacaaat gataaaacaa aaatatttat
     1561 atcatctaaa aacatgggtt taacaggtta tgaactagaa aatattttga gaaataaata
     1621 taacatacaa gtggagttat ctaattatta tggagttctt ttaatatgta caataggaaa
     1681 tactgttgat gatttctcta gtttagaaac agcattaaac aatattgtaa aggaagactt
     1741 taaatttgaa aagttagaaa ataaaaaata ccctgttgtt attccaaaga aaatattaac
     1801 acctagagaa gcattttata aaactaaaaa aagtgttaaa atatatgaca gcataggtaa
     1861 agtttgtggt gaatgtatag ttccataccc acctggaata agtgtaatat ctcctggtga
     1921 agtaataagt aaagaaataa tagactatct aatgttttgt cattctaaag gcatgattgt
     1981 gagtggattg aaagatgtag atttaaattt tatacaagta atagatttag aagacattta
     2041 aaaattatga tataaaagta gatagaagta gatagtttaa aattgtgaag agaaataaat
     2101 agactgctta tatagagata tctaataaaa gtactaaaaa agtaaaaagt gtttgtatgg
     2161 aggatatgaa aatgaaaggt aagttaataa ttatagagag tggttcagat gcaagtggaa
     2221 aggctactca gacagaaata ttatatgaaa gattaaaaaa agaaggctat atgattaaaa
     2281 aggtagaata tccaaattat gaatctccat catctgcctt ggtgaaaatg tacctaaaag
     2341 gagactttgg taaaaatgca tcagatgtag acccatatat agcatcaact ttttttacag
     2401 ctgataggta tgcatcattt aaaaccgatt gggaggagtt ttacaatgaa ggtggaataa
     2461 ttatcgcaga tagatataca acctctaata tggtacatca ggcctctaaa atggatattt
     2521 gtgatagaga agagtattta aattggttag ctgattatga atttaattta tttaagatac
     2581 cacaacctga ttgtgttata tttttagatg tgcctattga atttagtaaa aagcttatgg
     2641 agaatagaaa aaataaaatt acaggggaag aaaaaaagga tattcatgaa agtgacatag
     2701 agtatttaga gaaatcttac aacaatgctt tatatatagc agataaatat gattggaaaa
     2761 aaataaattg tgttttagat aacaaattaa gaagtattga aagtatacat aatgaaattt
     2821 ataaaatagt tcttgattca ataaaatcta tggagagata aatatgtatt ttgaaaatat
     2881 tatagggcaa gattttgcaa agaaatattt aacaaattct ataaagaaaa ataaacttaa
     2941 taatgcgtat atgtttgaag gaatggatgg aataggaaaa aagaaatttg cagatgaatt
     3001 atcaaaacta ttacttgatt atgaaaatct cgaaaatagt ccagactatg tactcataaa
     3061 accagatgga aatagtataa aaatagctca aatcagaaat ttacaatcag atatagtaat
     3121 aagacctcat aaagactata aaatatatat aatcaataat gctgaaaaaa tgactgtgga
     3181 agctcaaaat gcacttttaa agacgctaga ggaacctcca aactatgcta taataatctt
     3241 agtcacaaac aacaaggaat ctttattaga aactataaaa tctagatgtg atataataaa
     3301 gttctcacct ataccaattg aagacttaaa acgttatttg ataaatacag gtatagaaga
     3361 agaaagagct caattattgg ctattttttc gagaggaagt attgagaatg ctctaaatct
     3421 atctcagtct tctgagtttt caatgatgag agaagatata caacagtata ttcagataat
     3481 gttagataaa aacatagtgg agattttaaa tataccaaat aatatggaaa aatatagagg
     3541 taagattata gctttattag atatgatgat aaactatttt cgagatatca tcctattaaa
     3601 agaaaatgta aacaagaaca tgttaataaa tgtagataag ctagttttca ttcaaaatat
     3661 gagtggaaaa attagttatt ctcaattgtc caagattatt gatataatag aagatgcaaa
     3721 gagtaaaata aaaagcaatt gcaatttcaa tataagcata caagttatgt ctttaaatat
     3781 atacgaggtg attaaatgat aaagatagta ggtataaggt ttaaaagtgc tgggaaaata
     3841 tattattttg accctgttga ttttaatata gagcaagata tggatgtagt tgtagaaact
     3901 gcaagaggct tggagtatgg gaaagtagtc gttggtccaa aagatatgga tgaaagcgaa
     3961 ttagtttctc ctttgaagcc aattataaga atagctacag aagaagataa aaaagtatat
     4021 ctagaaaata aggaaaaggc aaaagaaact tttgagttgt gccaacaaaa aataaaagaa
     4081 catgagttaa caatgttttt aatagattgt gagtatacgt ttgataggaa taagttgatt
     4141 ttttacttta cagcagaagg aagaatagat tttagagaat tggtaaaaga cttagctgct
     4201 atatttaaaa ctagaataga acttagacaa ataggtgtca gagatgaagc taaatctata
     4261 ggaggtctag ggccttgtgg tagaaaactt tgttgttcat cttggttagg tgattttcaa
     4321 ccagtttcaa taaaaatggc aaaagaccaa agtctatctc taaatccaac aaaaatatct
     4381 ggaatatgtg ggagattatt ctgttgctta aaatatgagc atgatgtata tagtgaagca
     4441 atagaaaaaa tgcctgttgt aggtgcattg gtgcaatcag atgatggaaa aggaaaagtt
     4501 gtagaagtga atcctttatt agaacaaatt aaagtggaat ttcaagacaa gacagtaaag
     4561 atatacacaa gagaagatat aaaaatactt agagagccta aaaaatgtga tggctgtggt
     4621 ggtaaatgta aggataatga tggattagat gaagccacac taagagagct aaagaaatta
     4681 gaagattaaa tttaaggtag ggcttttcct acctttttta aagcaattaa tgcttaaagt
     4741 tattaatatt tgaggagaat ttatatatgc aattaaaaga ttcagaaaga atagatgatt
     4801 tacaattaaa aggccttaaa attatacaag atactaatgg tttttgtttt ggtatagatg
     4861 cagttttatt agccaatttt actaaaataa aaaaagatgc caaagtagta gatttgggta
     4921 ctggaactgg cattatacct attttgatag ctggaaaaag tgaagctaaa aaaattattg
     4981 gagtggaaat acaagaagat gtatatgaaa tggcaactcg ttctataaaa ttaaatggat
     5041 tagaagaaag agttgagatt ataaatgaag atataaaata catagacaaa gtcttagaca
     5101 taaatggtta ccatgtagtc acatcaaatc ctccatatat gcatattgat ggaataaaaa
     5161 atccaaatga taaaaaagca atatcaagac atgaagtaag gtgtaatttg gaagatgtaa
     5221 ttagagctgc atccagattg gttatgccta gaggaaaatt cttcatgata catagaccaa
     5281 caagattggt tgatataata actttaggga gaaaatataa attagaacca aaggttattc
     5341 aatttgtgca tccaagacct caaaaagcac cgaacttagt tttggttcaa tttgtaaaag
     5401 atggaagacc agagcttaaa atattggacc cattgtatgt ttatggagaa gatgggaatt
     5461 atacaaaaga attaaaagca atatataata ataaagatat aggagagttg taaatgagcg
     5521 ggaaattgta catatgtcca acaccaatag gaaacctaga agatataaca tatagaactt
     5581 taagaacatt aaaagaagta gacttgattg cagcagaaga tacaagacat agtataaaac
     5641 ttttaaatca ttttgaaatc tcaaaaccac taactagtta tcatgaacat aataaggatt
     5701 caaagggaga ttatttgata aataaactaa ttgatggtga aaatatagct ctgataagtg
     5761 atgcaggtat gcctggtata tctgacccag gagaagagat aataaaacaa gctatacaaa
     5821 ataacataga gatagaagta ttgccaggtg caacagcctt tgtaacggct ttagttggct
     5881 ctggtatgga cacacataga tttgtttttg agggcttttt agatagagat aaaaaagtta
     5941 ggaaagcaca attggaagaa ttaaaggaag aaagtagaac tataattttc tatgaatcac
     6001 cacatagatt gaaggataca ctaaaagata tgcttaaaat tcttggtaat aggaatattt
     6061 ctataaatag agaacttaca aaaaaatatc aagaaataat aagagaaaat atagaaacta
     6121 ccataaaaat atttgaggaa aaagaagtaa gaggagaatt tgtattaata gtagagggtt
     6181 ttcatggtga gaagtcaaag aaagaaaatt atgatgggct tacagatagg gaatatgttt
     6241 taaaactaat tgaaagtggt atgaacaaaa aagatgcaat aaaaatagtt tgtaaagata
     6301 gaaagttaaa gaaagatgtg gtttataaac aagttttaga tttataaaac ctgaatctga
     6361 ggtgatttta tggaaatagg gaaaattata atagagagtt taaagaggga agatacacat
     6421 aaaatattta tagaattaga taataagcat gaattagaaa aaataattcc taaaataagc
     6481 tgtatgaagt ctgtaggtga atgtaaatat catgtagtaa attgttttga acattctata
     6541 aacgcattaa aagaattaga aatagtatta aatgataagg atttttttcc aatacatcta
     6601 agaaaacatg tgtcaaacta tttaaacaca tatattgaag aaggaataaa taagttacat
     6661 gtattaaagc tgggaacatt tttacatgat ataggtaagc ctgacagtat gaccttagat
     6721 gaaaccggaa gagttcattt cacaaatcat gaaaaaatag gtgctcaaat aattgacaat
     6781 atgggaataa atttagagtt atcaacagag acatatagac ttataagtaa atatgtaaga
     6841 tatcatatga tacttttgtc tctatataag aaaaatgatt tgagtaggaa agaattaatt
     6901 aatgtattta acttagtaga tgaggacact ataggtgtaa taatgcttgg gtatgcagat
     6961 atagtgtcta cagtaaaatt attgggtaag agtggagaag tcagtgtgtt aaagacatat
     7021 atggaatata ttttaacaaa ttacttgtat aagagtaatt atacacatgt ataaatttag
     7081 ttaatataat tgagttttag atgattaatt aaagtttgaa atatacggat ttcaaaaaca
     7141 taaaagaagt tgtctttgtt gtaaaattat gaaagacaac tttttttata tcaaaaattg
     7201 aaattttatt aaggatactt aatggtctat taattaataa actattagta tttaatttat
     7261 attacaatat tgattttgct tgtattggcg ttgatagtat aacagaggtc ttagtagaac
     7321 caattttctt tatagtatca atcacatttt ctagctcgta catatctttt acaattactt
     7381 ttaaaagtaa gcaatcatca cctgttatat ggtgacattc tacaatccta gggtcctttg
     7441 cagctgactc aataaattct gtatatccat tgcttggaag agaaatatga ataaatgcct
     7501 ttataactct gcctaatgaa tctgggttga caatagcttt atatccttct ataacaccag
     7561 actcttctaa tcttttgact ctttctgaaa ctgcaggaga agttaaacca actatttttc
     7621 ctaagtcctt catagaaatt ctcccatcat cctgtaaaat ttctatgatt ctgtaatctg
     7681 taacatccat aaataaacag tcccccttaa ataaaaataa ttaatgctat aaaactttct
     7741 tagaatctat catttgtgtt tttaaatacc aaagatttct agataaatca accttataaa
     7801 tgtgtggttt tgaaagtctt ttgtattgtt cccagatagt acttaattca tattgtacat
     7861 gattttttat ttcaagaaca gcttttttat tatatttgca tctgcctttt atatacaatg
     7921 gtttaagaag ctctttaact ttgtaatttg taaatacctt cgttttccat gtataagtag
     7981 gatggaagat ttctaaaggt ttagactcat ctataacttc atcatgtaac ataatcaaat
     8041 ctgcctctgc cttattgtct tcgttatata ttcttataac tttcttgtaa ccaggattat
     8101 ttattttttc tggattctca gaaatcttta tcttaggttc aagaactcct ttttcacaag
     8161 atgcagctaa tttataaact cctccaagag aaggtgaatc gaaagatgta atgagctttg
     8221 tacctactcc ccatgaattt atagtagctc cttctgcttt aagtgatgtt atggtatatt
     8281 cgtctaggtc attagatgca gtgatgctga tatctgaaaa gccagcgtta tctaattgtt
     8341 ttttagcttc tttagataaa taagctaaat ctccagagtc aagtcttata cccataggtt
     8401 tgtatccttt ttcagaaata tttttgaata cttttattgc attaggaact ccactgttta
     8461 atacatcata agtatctaca agtagtaggc atttatctgg ataaacatca gcatatgctt
     8521 ggaatgcttc aagttcatta tcaaattttt gtacccaact gtgagcttgt gtaccaatta
     8581 taggtatatc aaacattttc cctgctagta cgtttgcagt tccagcacaa ccaccaacta
     8641 cagcagccct agccccatat atccctgcat ctggaccttg tgcacgacgt aatccaaatt
     8701 caaatacagg gtctccttga gctgcaaagc aaactctaga ggctttagta gcaataagag
     8761 actgaaaatt aactattgtt aacaatgctg tttcaataag ttgagcttga tataaaggtg
     8821 ccttaacagt aattaatggc tcattaggga acatgatagt tccttcctca acagcatata
     8881 tatctcctgt gaatttaaat tcttttaaaa attttaagaa cttgtcagaa aataaattta
     8941 agttttttaa gtactctaaa tcttcatctg aaaaatgaag attatctatg tattcaacaa
     9001 cctgatcaat accacaaact atagtatacc ctccatcaca agcgtttttt ctaaaaaaca
     9061 tatcaaatac aacaatgtct tcatgaatat tcttttcaaa atagccattt aacatagtaa
     9121 gctgatataa atcagtaaga agtgttagat ttctcattat ttctctcctt aattattatt
     9181 aaatattaaa aattgtaaaa actcccacac ttatactaaa acataatata gtacaacttt
     9241 caagtataaa gtattaccta actatcataa aattatttaa agaggaggat gattataata
     9301 ggaaaaatat ggttttatat ggtatcgata ggaataatag gaagcttgat ttctaaaaat
     9361 atgggtgaat taaataaagt tatattaagt gaaacatcaa aaggtataga gtttgcaata
     9421 agtctagctg gtatcatggc actctggatg gggataatga atatcgcaaa agaatcaggt
     9481 ttaattgaaa agataggtca aaaactaaat cctataatga gaagattatt tccttctatt
     9541 ccaaaaggtc ataaagcaat gtcttatata gttatgaata ttgctttaaa tatgttgggt
     9601 gctggaaatg gtgcaacagc atttggttta aaagcaatga gtgagcttca aactttgaat
     9661 aacaagaaag atacagccac taatgatatg attatgttta tggtaatcaa tatatcatct
     9721 atacaaataa ttccatttac tataatcaaa ctaaggatgg atatgggggc tcaaaatcca
     9781 agtgaaataa tttttacgac tttatttgca accataatat ctacagcagt agcaataata
     9841 acatgtaaat tttttcaaag aagatataga taaaggggat gtagatacat ggagatattt
     9901 tcaaacttac ttataccaat aataattctg tacatagtag tttatggtaa atataaaaaa
     9961 atagatgtgt atgatagttt tgtaaaagga gcaattgatg ggttaaaagc agcatgggat
    10021 atcctaccat atataatagg tatatttttg gcaataggaa tattcaaaac tggtaaaggt
    10081 cttgatatgt tagaatggct atttacacca atagcaaata tgatgagtat accaaaagaa
    10141 ttaataggtc taataagtgt aaaaccttta tctggaagtg gtgctttagg gatgtacagt
    10201 gaacttgcaa atagagttgg aataggaagt ttagtagaaa aaatgggtgc tacgattgta
    10261 ggttcttctg agactatttt ttacacaatg gcaatatact atggaagtct gaagataaaa
    10321 aatactagac atacattatc atgtgctatg attagccatg ttgcaggtgt catagcagct
    10381 gtttttattt gctatgtaat atttgtataa tttgttgaca atatgggaaa aattttataa
    10441 tatagtgatt atatagaaac aaatagtaat acaaattaca agctatgaaa agaagagtaa
    10501 ataaaggttt tttctatcag agagctaggt taggtgaaag ctagtgtcaa aagctttatt
    10561 gaagatggtc ttggagcttt ttatctgagc aagtgtgcta gggtaaaacg gttggacacg
    10621 ttatagtcta ctaaggtatc tataaaatgt ttctacattt catgtgaaac ataactatta
    10681 tagatgaatt agggtggtac cgtgaataat ctcgccccta tgttaactca taggaggcga
    10741 ttttttatta tttaaatgaa agaatcatat gattacgaac gataaaaaat taggaggtaa
    10801 ataacatgag caaaccgagt ttttatgtaa caacaccaat atactatcca agtggaggat
    10861 tacacatagg acatacttat tcaacagtag ctgcagatac aatagctaga ttcaagcgtt
    10921 tctgtggata tgatgtaaag ttcttaacag gaacagatga gcatggtgaa aaaattcaaa
    10981 agaaagccat agaacaagga atgtcagaga tagagtatct tgatggaatg ataaaagata
    11041 taaaggcctt atggaatact atggatatat cttatgatga ttttataaga actacagaaa
    11101 aaagacatac agacataata caaaagatat ttactaaact atatgagcaa ggtgatatat
    11161 ataaaggtga atatgaaggt agatactgta ctccttgtga gagtttctgg acagaatctc
    11221 aattactaga aggaaataaa tgccctgact gtggaagaga aacatattta gtaaaagaag
    11281 aatcttattt ctttagatta tctaaatatg aagacagatt aaaagagtta tttaaagatg
    11341 atagtttctg ttttcctgct gctagaaaaa atgaaatggt ggcaaacttt ttagacaaag
    11401 gattagaaga tttaagcgta actagaacga cttttgactg gggtataaaa gttccttttg
    11461 atgaaaaaca tgtaatttat gtgtgggtag atgctttatg taactatata acagcattag
    11521 gttatatgac tgataatgat gaagaattta aaaaatattg gcctgccaat gttcaaatag
    11581 taggtaaaga aatagtgaga ttccatacaa taatatggcc agcgctttta atggctttag
    11641 gtctagaagt tccaaaacaa gtatttggac atggatggat actatttgct gatgataaaa
    11701 tgagtaaatc aaaaggtaat gtggtttatc cagaacctat aatagaaaga tatggaatag
    11761 acacacttaa gtacttctta ttaagagagt ttgcatttgg gcaagatgga agttatactc
    11821 atagaaattt tgtaacgaga ataaattatg atttagcaaa tgatttagga aacttaataa
    11881 gtagaactgt agctatggtt gaaaaataca ataatggtat aataccaaca gcaaaagttt
    11941 caactaattt tgatgctgac ttaaaagaac aagctgtttc aactagagaa aactttgaag
    12001 ctgaaatgga taaaatgcaa ttccatgaag cattagagag tgtatggaaa ttagttagaa
    12061 gaactaacaa atatatagat gaaactatgc cttgggcact agctaaagat gaaactaaga
    12121 aagatgaatt agacacagtt ttatataatt tatgtgaatc tataagaata atcgcaacac
    12181 taataaatcc tataatgaat gagactgcta ataaaatata tgaacatata ggaataaaag
    12241 gtcaagatga tataactaca tgggaaagta caaaaacatt tggtcttatt ggagaaaatg
    12301 taaaagtatt taaaggtgaa cctttattcc caagattaga tgtagaaaaa gagatagaag
    12361 aattaactaa aatgttctct gggaaacctc cagtagaaga aaaaccttta gagcataaag
    12421 aagaaattac tattgatgat ttagacaaaa tagagcttag agtagggaaa ataataagct
    12481 gtgaaaaaca tccaaaagca aataaattat tagtatcaca agttaagatt ggaccagaaa
    12541 caagacaaat agtatcagga atagctgaat actataagcc agaggattta gtaggaaaag
    12601 aggttacagt ggtatgtaac ttaaaaccag ttaaattaag aggtgtagaa tctcaaggta
    12661 tgatattagc agcaggtgat gatggagacc catatgtttt accatttaca caaggggcta
    12721 aagatggatg cgaggttcgt tagtaggtaa ttaaatagta aggagattaa agatgttatt
    12781 tgactcacat gcacatttaa atgatgaaag ttttgatgaa gatagagatg aacttattgg
    12841 ttcattaaaa gataaaggtg tagatttggt tgtaaatcca ggagcagata tagagacgtc
    12901 gataacagca attgaacttg cgaaaaaata cgactttata tattcagcag ttggggtaca
    12961 tccacatgat gtatcaaaac tagatgatac agcaatagaa actctaagaa aacttgcaac
    13021 tgagaatgaa aaagttgtag ctattggaga gattggatta gattattatt atgattattc
    13081 tccaagagaa gaacaaaaag agtggtttaa gaagcaaatt gaattagcta atgagctaaa
    13141 acttccaata ataatacatg atagagatgc acatggagat acttttgaga taataaagaa
    13201 tactaaaaat cctgaaatag gatgtgtact tcattgttat agtggaaatg tagagttagc
    13261 tagagaatat gttaagatgg gttgttatat atcaattcca ggcacggtta cttttaagaa
    13321 taataaaaaa actagagaag tagtaagaga aatacctcta gaaagattat tcatagagac
    13381 tgattcacca tatatgtcac cagagccaca tagaggaaag agaaacaatc cttcccaagt
    13441 atcttttgtt gctgataaga tagcccagga aaaagggata tcttatgaag aagtatgtag
    13501 agttacaaaa gaaaatgcaa agaaattttt taatataaaa tagacaaatt aaatcttata
    13561 atgatatata taataagtca ttataagtat aggatgagag tgcctcaaaa tgagtttttt
    13621 agtttatgag gcactctttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa
    13681 aaagaagttt atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa
    13741 tatattttct taacattgtt aaaaaagaat ttacaagata ttcattagaa ttaactcaac
    13801 tctaatattc acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt
    13861 agattaagga ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa
    13921 cttagagaaa ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat
    13981 taaagaacct aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac
    14041 atttaattta ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg
    14101 ttacggaata gtttcagaaa acaataataa gaaagccatt tacttagcag ttatagatgc
    14161 agttttacat agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt
    14221 atatgaagag aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa
    14281 aacgaaagtt caattcaatg ctatggatta aataggagga gaatcatggt aaattataca
    14341 caagaaatat atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata
    14401 gataatataa atatagaaaa taatacaaaa ctattgaatg gagcaattgg tatagcaatc
    14461 actttattag accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa
    14521 aatataagag cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt
    14581 ttagatatta attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct
    14641 gatgaaagtg caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag
    14701 tatataaagc ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat
    14761 ggaattagag aagaatttat agaaagatta tctaccataa ataaagatta tccaatagga
    14821 atagatttga ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa
    14881 ttaagttggg aggtattgta gaaatggctt atgtagcagt taaaggtgga gaagaagcca
    14941 tattacaggc taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag
    15001 atgtaaagca gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa
    15061 gtttatacaa caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag
    15121 aagcctcttt ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac
    15181 ccataaatac agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac
    15241 caggaggtca gtatcttggg gcaactagag attatgaaaa tagaatattg aatgtagaat
    15301 tattagaaga agatgaaaaa aagaatagag attttagaga tagttttata aatgaattag
    15361 aaacagtaga tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt
    15421 tattacaaga agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa
    15481 atagtttaag atttccagtt tctaggtcag caaagttaca ggcattaact agaggtgaaa
    15541 gtggagtagt gtcaggtttt gcttatgcta ttgtaagagg ttttggtgat gaacatccat
    15601 atataagtga acttagaact ggtaatgtat ttataaaagt aaaacatccc atctatgaag
    15661 atgaagaaat aataataggg gatatgctgg ttactgaatg tgaaatagtt tcaaaagcat
    15721 cacaaaataa gactgaaaat tctaaaacta attcggaaga taaaaataag atgtctttag
    15781 gatatggtct ttgtatgggg aataatgaga ataaggctat atctatggcc atactagaca
    15841 aatctttgga gccaaatagt gaaaaactag cacaagatga agaatttgta ttgcttcata
    15901 tagatggaat agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt
    15961 ttaaagcgga tgtagaaaga ataaaatagt ttgtggaggt aagtttaggt gatatataac
    16021 tatggttttt tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata
    16081 tcaatacctg gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg
    16141 ggtactggag gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta
    16201 attgaccaag gaaatgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt
    16261 gtaagtaatg tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat
    16321 agaataccag aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca
    16381 gaaaccctta gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa
    16441 gattatagta ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct
    16501 atagcagttg aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata
    16561 cctagatttg atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt
    16621 agagaaaaaa gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag
    16681 gattataagt ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca
    16741 aatacatttt tagatgaagt atatgacagt gacactagtg aaaagtacta tagttgctca
    16801 gatacaagtt attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga
    16861 ggtacttgga atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt
    16921 attaatagga agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag
    16981 cacaggatat gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa
    17041 tgtaaattta gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg
    17101 aaaaagcact ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata
    17161 tatatcccat tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata
    17221 cataaagaat cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga
    17281 ctttagtagt ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga
    17341 aaaaataaga gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat
    17401 agaccataaa ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc
    17461 tctttcaaat aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc
    17521 tgtgcaagcg gaagtattgg atttaataag agaaatccaa agagaactta agatttctat
    17581 gatagttgta tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat
    17641 gaaaaatgga agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca
    17701 cccatatact cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa
    17761 tgggggtttt taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact
    17821 ttgagttaca tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt
    17881 caaaaggtga gtttttagga ataataggta aatctggagc aggtaagtct accatattaa
    17941 aaagtatcta taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat
    18001 atggtaatgt agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag
    18061 aaataggata tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg
    18121 tagtacattc tttaattgag agtgggtttg aaaaagattg ttcgtatgat atggccaaag
    18181 atatacttaa ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt
    18241 ctggtggaga aaaattaaga ttaaatttag ctcaagctat ggtcaaaaag cctagacttt
    18301 tgttacttga tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa
    18361 aaatgttgga gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt
    18421 ttatggaaac tgttatagat aagaccttta ctatggcaaa agggactata agtgaaagtg
    18481 gggtagccta gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt
    18541 tagaaaatgg tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga
    18601 ttgattacgc atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg
    18661 taatacctgg tattatagat attcacagtg atgccattga aaaggaaata gagccaagac
    18721 caagtacatt attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa
    18781 atggaataac tacagtttat cattctatat ctttagggga tggagtagga gttagaagta
    18841 ttgataactc tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga
    18901 gcataaatca taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg
    18961 ttttagaact attagatgaa aataaaatag actacttatc aataatggac cattctccag
    19021 gacaaggtca gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag
    19081 ttacagaaaa ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag
    19141 attggaataa aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat
    19201 cacatgatga tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat
    19261 ctgaatttcc tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt
    19321 gtttaggtgc accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg
    19381 atgctatatt ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga
    19441 taaagtctat gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat
    19501 ttacctcaaa tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa
    19561 ataaaaaagc agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata
    19621 ctattgtaaa tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac
    19681 attgtcatac aagtatatca gataataatt ttactactga agaaataata aaaaaagcag
    19741 cagaaaagaa tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg
    19801 aagctcttgt gcttggagag cattatggaa taacaataat accaggtata gaaatttcag
    19861 cctatgatta taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca
    19921 gtcaagaaat aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta
    19981 agatggtaaa taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat
    20041 attcatcaaa gactggaata tttaaacaac atataatgaa agctcttata gataaaggat
    20101 atacagataa tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg
    20161 tatatgtatc tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg
    20221 gtggaatttg tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga
    20281 tggtaaaagt aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag
    20341 aaaaagagtc gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt
    20401 accacggatt ttattctcaa cataattatg aacttggaag caagagctta aataaagaag
    20461 atttatttaa gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa
    20521 aaaatgtagg taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg
    20581 aaaaaggaga gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta
    20641 gaataattgc agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata
    20701 tgactaattc accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat
    20761 ttccaaatat gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta
    20821 aatctaatat tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg
    20881 agaaaaaata tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg
    20941 cgattgcttt agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa
    21001 aagtaagact taaacttagg gagcagatta gaagtttaca tagaaaacta ggcattacaa
    21061 ctataatggt aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta
    21121 tgaatagagg agaaataata caagttggaa caccaaagga ggtatataaa aatccagaaa
    21181 caccttttgt agctgatttt ataggaacaa taaattttat agatgatggt attaataaga
    21241 tagctattag accagaagat ataaaagtag agtctaatag agattctaaa gataaggata
    21301 taaaagtggg agaaatttta gatatcgaat ttagaggatt taactataga attacagttg
    21361 agtatcgttc taagcaaatg aaattagatg tagtttccaa agttgcagaa caaatgaaac
    21421 tatgtatagg ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat
    21481 cagagggatg tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag
    21541 aataagtgat agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt
    21601 atttatatta tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa
    21661 ttttatagga atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt
    21721 taaaaattca atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat
    21781 atattcatac gcaataacaa gaacaaacat aaagtttaaa gattttttta aaactatggg
    21841 aatgtttcca ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg
    21901 aaataaggga atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat
    21961 aattatttct cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact
    22021 ttcgatggct gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa
    22081 gaaattttgc aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt
    22141 atcctttata cttgcattta cagattttgg agcagctaag gtagtcggag gaaactacaa
    22201 tgtactggct actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc
    22261 tactgtttct atggtaatgt taattcctgt actaatagca tttacattag ataagatggc
    22321 aagtaagaaa caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa
    22381 taagtttaga gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat
    22441 tttaatattc gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt
    22501 tacaatgaaa cactatacat ttaaaggtat tttgggaagt ggaataaaaa catacttaag
    22561 cagtttgaaa atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc
    22621 ttatatgata gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc
    22681 aatgatacca atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa
    22741 ctcagcaagt aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa
    22801 tgtagttcat ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga
    22861 taaagagtat gaattagttg caaaatccat aaatattcca ttttataaag tattttttaa
    22921 tataacagtt ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa
    22981 ttcaatggtt actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc
    23041 aatatcaata ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt
    23101 attagtgctt ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt
    23161 aaaaaataac acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa
    23221 tttaagaagc tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga
    23281 tgtagtactg attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta
    23341 acagtttaca cagcaattga agaagatagt atagagccat atttagctac atttaaagag
    23401 aaatatccag atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga
    23461 ttattagcag aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc
    23521 ttattggttg cagatgacca ggggatgtta gaactatatg ctccaaaagg ttctgaagaa
    23581 atagagtctg catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg
    23641 atgacaggta taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt
    23701 tcttacgaag atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct
    23761 tcttctggta cagggtactt aacagtatca gcccttatac aaattatggg tgaagaaaaa
    23821 gcatggcagt atatggataa gttacatgag aatattggtg tatacactca atctggttca
    23881 gctccagcta catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga
    23941 ggtataaagt taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct
    24001 ggatgggata tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa
    24061 gtatttttag attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca
    24121 gttactacta tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag
    24181 caaatgatag ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa
    24241 tggatatcaa aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg
    24301 agtaaaaaga aaaatttttt taagtagatt ttattataat aatataagtt gagaataata
    24361 taggtcaaga aaagtgaata ttctataaaa atgtatagag taagtatacc attcagtttg
    24421 aatggtatat tt
//
LOCUS       DAEMIK010000052        23160 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEMIK010000052 DAEMIK010000000
VERSION     DAEMIK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.052"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            31..1296
                     /locus_tag="KPK87_001745"
     CDS             31..1296
                     /locus_tag="KPK87_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG2537897.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            1335..1790
                     /locus_tag="KPK87_001746"
     CDS             1335..1790
                     /locus_tag="KPK87_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537898.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1934..2305)
                     /locus_tag="KPK87_001747"
     CDS             complement(1934..2305)
                     /locus_tag="KPK87_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG2537899.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(2308..2676)
                     /locus_tag="KPK87_001748"
     CDS             complement(2308..2676)
                     /locus_tag="KPK87_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2537900.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            complement(2946..3824)
                     /locus_tag="KPK87_001749"
     CDS             complement(2946..3824)
                     /locus_tag="KPK87_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2537901.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(3841..4158)
                     /gene="trxA"
                     /locus_tag="KPK87_001750"
     CDS             complement(3841..4158)
                     /gene="trxA"
                     /locus_tag="KPK87_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2537902.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(4308..5540)
                     /locus_tag="KPK87_001751"
     CDS             complement(4308..5540)
                     /locus_tag="KPK87_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2537903.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(5530..6201)
                     /locus_tag="KPK87_001752"
     CDS             complement(5530..6201)
                     /locus_tag="KPK87_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537904.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(6217..7053)
                     /locus_tag="KPK87_001753"
     CDS             complement(6217..7053)
                     /locus_tag="KPK87_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537905.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(7079..7753)
                     /locus_tag="KPK87_001754"
     CDS             complement(7079..7753)
                     /locus_tag="KPK87_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2537906.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(7767..8495)
                     /locus_tag="KPK87_001755"
     CDS             complement(7767..8495)
                     /locus_tag="KPK87_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2537907.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(8622..9311)
                     /locus_tag="KPK87_001756"
     CDS             complement(8622..9311)
                     /locus_tag="KPK87_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2537908.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNYFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(9319..9882)
                     /locus_tag="KPK87_001757"
     CDS             complement(9319..9882)
                     /locus_tag="KPK87_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2537909.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPI
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(9884..10852)
                     /locus_tag="KPK87_001758"
     CDS             complement(9884..10852)
                     /locus_tag="KPK87_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG2537910.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYENIIDSVEGVI"
     regulatory      complement(10953..10996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11361..11990
                     /locus_tag="KPK87_001759"
     CDS             11361..11990
                     /locus_tag="KPK87_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2537911.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            11965..12369
                     /locus_tag="KPK87_001760"
     CDS             11965..12369
                     /locus_tag="KPK87_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG2537912.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            12372..12848
                     /locus_tag="KPK87_001761"
     CDS             12372..12848
                     /locus_tag="KPK87_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG2537913.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            13395..14060
                     /locus_tag="KPK87_001762"
     CDS             13395..14060
                     /locus_tag="KPK87_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG2537914.1"
                     /translation="MIKLIGILIVIIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            14082..15014
                     /locus_tag="KPK87_001763"
     CDS             14082..15014
                     /locus_tag="KPK87_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG2537915.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKSATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            15035..15676
                     /gene="pcp"
                     /locus_tag="KPK87_001764"
     CDS             15035..15676
                     /gene="pcp"
                     /locus_tag="KPK87_001764"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG2537916.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(15796..16128)
                     /locus_tag="KPK87_001765"
     CDS             complement(15796..16128)
                     /locus_tag="KPK87_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2537917.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            16393..17013
                     /locus_tag="KPK87_001766"
     CDS             16393..17013
                     /locus_tag="KPK87_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2537918.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     TGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKTGIKEPQYEEIIMTNFIK"
     gene            complement(17338..18162)
                     /locus_tag="KPK87_001767"
     CDS             complement(17338..18162)
                     /locus_tag="KPK87_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG2537919.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYALMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(18173..19576)
                     /locus_tag="KPK87_001768"
     CDS             complement(18173..19576)
                     /locus_tag="KPK87_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2537920.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNTIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(19580..20305)
                     /locus_tag="KPK87_001769"
     CDS             complement(19580..20305)
                     /locus_tag="KPK87_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2537921.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(20417..21190)
                     /locus_tag="KPK87_001770"
     CDS             complement(20417..21190)
                     /locus_tag="KPK87_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2537922.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIAISIILMIVLTKITYENFTSSKRF"
     gene            complement(21196..22083)
                     /locus_tag="KPK87_001771"
     CDS             complement(21196..22083)
                     /locus_tag="KPK87_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537923.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(22097..23077)
                     /locus_tag="KPK87_001772"
     CDS             complement(22097..23077)
                     /locus_tag="KPK87_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537924.1"
                     /translation="MFISLGILVLILMISIFITPVLETESYYIDKEKGRIEDTRSGID
                     IGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNEYKDISFWKVFNYRV
                     TNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALKSKIFVALLVPLVIY
                     SVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKYVILNIGIELLMFEG
                     WAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLSVVSNVNYYDLIFGF
                     NKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIKSDYISR"
BASE COUNT         8024 a         3624 c         2580 g         8932 t
ORIGIN      
        1 caaaataata caaatggagg tacttatatt atggaatttc aacaaataat agctatatgt
       61 atatttttaa tagttatggc agctattatt acagaaaagg taaacagatc cgtagcagct
      121 gttggtggtg cactattaat gataatattc aatatactta cacttgatga aggattaagc
      181 catatagatt tcaatactat tggtgtatta gttggtatga tgctatttgt agctgttgtc
      241 aaaaactcgg ggttatttga atatatagct atctggactg caaagaaagc taaaggtgac
      301 ccatggaaga taatgatttg ttttgctatt ataacagcta ttttatcagc tgtattagat
      361 aatgttacaa ccgtcttgct tattggacca atgactatag ttattacaca aatattagga
      421 cttaatccag ttccattttt aataacacag attttagctt caaatatagg aggaacagct
      481 actcttatag gagaccctcc aaatattatg attggtagtg ctgccaatct tagttttatg
      541 gattttgtaa taaatttagg tcctgctgtt atagtaatac ttgctataac tataatatgc
      601 tttagattta tttatggaaa agaacttgtt gtgaatgaaa gagctaaaaa tgcaatatta
      661 aaactagatg aaaaaaaatc tgttaaagat aaacctctac ttataaaaag ccttatatta
      721 atagcattca tactttttgg ttttatgttt cacagtacaa tccacataga ttcttctgtt
      781 gtagcactga caggagcatc tataatgctt ttaataggaa aacaagatgt agatgaaatt
      841 atggcaggta ttgaatggtc tacaatttta ttctttatgg gattatttgt agtagttggt
      901 ggattagtag aagttgggat aataaacaaa ttggctcaag cattgattgg acttacagaa
      961 ggacatcttg tatttacaat gttgttgata ttatggttat cagctatagt ctcttctttc
     1021 ttagataata ttccatttgt tgcaacacta attcctctta ttttgactat gcaagctgaa
     1081 ggtattgatg taatgccact atggtgggca acatctcttg gagcttgtct tggaggaaat
     1141 ggaacactaa taggagcatc tgctaatgtt gtactagcag gtataggtaa taaacacggt
     1201 catcctatct cttttaagga gtatttcaaa ataggttttc cacttatgat tatatctatt
     1261 attatatcta ctgtttattt aattataaaa ttttaaaaaa tatagtataa taatactaaa
     1321 ggggatgtgt atatatgaat attaatactg ttcaaggtga ttcgattgaa gttcttttaa
     1381 gacagcttgg tgccacaaga atatcaaaag taagtagtac attatatttt atcaagtttg
     1441 atttaggaga cggttgggaa atatcttata cttataatat taatgctaaa gaccaatatt
     1501 ttttacagag aattgaacca tatcctattg gtagaggtct atttaatgat gaatatgaaa
     1561 tagtatcatt tatatcaaag gatttaaaaa aatttctaaa tgctaaaaat agtagtaact
     1621 ttaaaacatt tgttgaagtt actagaaaag tcaattctat cattgataat gtagaggaac
     1681 tatttttaaa ctacaatgta gatggtgatg atttaaaaac acttaataaa gaactcaatg
     1741 acatattaaa tgatattgac tatatagaaa aacatacaaa aaaaatctaa aagtagtaaa
     1801 cttgtaaact tgaaatatta ttaaggatac tacaaaaaac ataaaaattt aaattttggt
     1861 tttaaaagaa taaaatcgta tttattaaaa gaaaaaatag ctgatgaagt atatatttca
     1921 tcagctattt tttttaatga tgtccacatc cgtcatgtga gcaagtatgg tctgatggaa
     1981 actctactaa ttttccattt ttaagtaagt ctaaattagc acttatagta tcaccttctg
     2041 ctctgtaaac ttttattccc attccattta atttaattat agctccttgt cctattcctc
     2101 caacaactac tgcatctaca gctgtccctg acaatgcttt taatggttga cattttccat
     2161 gttcatgatc taaatctcca ttatctaaag atttaacttc tccactttct aaatcacaca
     2221 caacaaaaat tggagcagaa ccaaaatgtc catatggttt actatccaat cctttattct
     2281 cctctactgg tatacatact ttcattatta tttctccctt ctcttacaac atttttttat
     2341 agatttacat tctttactgc atcctagatg tattgtattt acatcaaata catctataaa
     2401 gctttcacta gaattactta acatagaatt tataaaatct acacatatat taaatgattc
     2461 tttattaata tgatataaaa catagtatcc ttctttatat cctgttatta tcccaacatc
     2521 ttttaatatt tttatatgtt gtgaaacagc agaatctgaa atccctagat atttagaaat
     2581 ccccttttga catatatttc tcttggatac taatataagt atgttaagtc tagtttcatc
     2641 actcaatgct ttaaatattt taaccaagtt ttccataagt acctcgtttt gattaagtaa
     2701 ttacttaatt aaattatagt actcttaatt agatttttca accattattt ttaaattttg
     2761 gagtatatta taaataattc caatttttac taatataact ttgagtttta ttaataaaag
     2821 taattacata tagattttaa aatactttta ttaataactt aaaaataaaa aactgtcttg
     2881 aaataataaa attatttcaa gacagttttt tataaatatc tttaaaaagt taatcaatat
     2941 aaatattaag ccctctttaa tttatctaaa tagtaaacag catttaaagc tgctatttgc
     3001 ccttgaccag ctgcttttat ataagaatat ggttttccaa cacaatctcc agcagcaaag
     3061 catccatcta tactagtctt catattattg tcaactttaa tatgaatgcc atcaatctct
     3121 attccaggaa ccaatgcact tggagccgta ctatctttta taacgaatac tccatcaact
     3181 tcaatgtggt tttctttaaa agatactttg tttacaagtt tatctccatc tatctgtact
     3241 ggtctgtcat gtataacttc aatagaatca tctaggttat ctgaacttct cataagatta
     3301 tcttttttgt acataggtat aaaataagtc tttgaagtga gttcacttaa aaaatttgct
     3361 tcttctttag attcttcatt atatcctata actgctacct ttttatttct ataaagtggt
     3421 gcatcacatg tagcacagta gcccacacct cttcctagaa attcttcttc acccttgatt
     3481 ggtctagtat actctacccc agttgcaagg attacagtag tagcttcata catatcattt
     3541 ccagacatta ttgtaaagta ttctcccata gcataaatat tatttattct tttattttca
     3601 attgaaatat ccatactatc tatatgtgat ttaaacttgt cttttaattc atcaccactt
     3661 atatcataaa aacctaaata attatctatt gaaggagctt taacaagctt attacttaaa
     3721 ttatcattac caaacatgat aattgtttta tttcttattt ttgcatttat agcagcagac
     3781 aatccagctg gaccactgcc gattatagct atatcatatc tcataatact cacccatttt
     3841 ttataaatat gattttactt gcatttcaat tttgttcttt ggcatgaatc caattaaagt
     3901 ttctactggt tttccatctt taaatataat catagttggt actgtagata cattgaattg
     3961 ttgagctatt tctaagctct catctatatc aacttttaca aatctagctt tatccttcat
     4021 ctcttcacct aattctgcaa atacaggtcc caacatgtta caaggtccac accaagttgc
     4081 aaagaaatca actacaacta ctcccttact accttcaaca ctacttctaa attcagatgt
     4141 atttataact tttgccattt ttaaatcctc ctattaaact ttattatccc tatataatta
     4201 tactatgaaa tttataaata taaatttact tttataaggt ctttttatat tttatactaa
     4261 ttagtatacc cattcttcat ttctaaaaac aattttttat atttttatta ttttaacttt
     4321 tttggtattg acaattcaaa tgtagttcca atatctacat cagaagttac atttatatca
     4381 atagataagt ttaaactaat catgcgagct attgaaagtc ctattccaga accttctaaa
     4441 tctttatttc ttacattttc acttctaaaa aatctatcaa atataagagg tatctcttct
     4501 ttttttatgc ctataccagt gtcacttata agtaaagtta cctcatcctc tatatcttct
     4561 ttcaattcta atgctatctc atcacccaat tttgtatact taaaggcatt atcaatgaaa
     4621 attaatataa gctgtcttaa tttattttta tctgttatta taactttatt cttcagttta
     4681 gaattaaaca cgaatctttt ttcctgaaac tcagcaatgt caatatagtc ttctgatatt
     4741 tcttccaata acttttctaa gtctatttct tctaaattaa ctttaacaat agagtcctct
     4801 ttagtaagag acaataaatc agttatcatt tttttaagtc ttctagtttc cttcattgca
     4861 gtagctattg tttcaacttc atcatttact gtactttctg gggattttaa catactttct
     4921 aattttgatg aaactattgt tattggtgtt cttaattcat gagaagcatc ttgtataaac
     4981 ttagcttgat ttttccatgc tgtttctatt ggaattaaag cttttctagt gagatataca
     5041 gccacaaagt aagtaattat tacagaaatt aatattccaa ttataagaac agatgtaagc
     5101 tgtcttaaag aattcatctc agaatctata tttctaatta tctgtatttg gtatctacct
     5161 acatctacat ttaattctct aaaagtatat ccattttctg tgtatttaaa aaacgaattt
     5221 tttttatcta gtcttctatc tggaagtaac tcatcaaaat attcattttg agtatagtaa
     5281 cttatccttc catcttcata tacatacacc atatctttag gatctttgag tcttattggg
     5341 ttcaaaaatg aagttctttt aaattgagag actatatatt caagttcatc atttatctca
     5401 ctatcaattc cactatatgt aagtccccta aaatatgagt atataaatat agaaaagatt
     5461 attaaaaaac tacctactac aataatatac atttttatta aatgattttt agtactggta
     5521 aatacattct tatttatcat cgaatatata tcctacctta cgtttagttt taatatattt
     5581 atcatatcca tatttactta gtttttttct tagattactt acatatactt ccactatttc
     5641 aatagtcgca tctgaatcaa ttccccatat tctatcataa atctgctctt ttaataaaat
     5701 tgaaccttta tttaatacaa aatactcaag caagttaaac tgtttgtttt gaagttttat
     5761 ttcttctttt tcaatataca ctcttttttc tagtgtatct aaatataaag atttaaattc
     5821 taatccgttt ctttctttta tttttccatt agtccttaat atagcgtata ccctagctac
     5881 taactcttcc atataaaaag gttttgtcaa ataatcatta gctcctatag taaatgcttc
     5941 tactttgtca tctaatgttt cttttgctgt caatataagt acaggtgtat ctatattatt
     6001 aactctcatt ttctttagga tatcaaatcc atttatatta ggaagcatta agtctaaaat
     6061 gactaaatca tatatatttt gatttactaa gtataaagct tcttctccat cttcacactt
     6121 ttcagtttca aaatgcttgc taagttcttc tactacactt tctaaaagta tcttattatc
     6181 ttcaactatt aaaaccttca taagtatccc ctacctttat ttgtctatct taactttatg
     6241 agattttaag tcaatatcca cattggcttt ttcttttata tcatcatacg tatatcccac
     6301 tatttcaaca ctttttgcct ttgttgaaca caatatatct atatctgtct cttctgaagg
     6361 ctttaaaatc ttatatgatg gcacacgatt tactgttaaa tattctccat tttcattaac
     6421 ttctgctgtc ttaagaataa tatttcctaa atcttttgat gatgaatttt ttacttttac
     6481 ttgatacgta attccctcat ttgattcatt tactttttta agttctgatg tagatagcac
     6541 ctcatactga ctactatttt ctagcttaat attattattt cttatattaa tagtattttc
     6601 ttttaaatta acatacacaa tcttaccctc tgcctcatat tcatatgcat aaacttctac
     6661 accctttaca aaagttttat gcccacattc tatgtaagca actttcccag gcattaaagt
     6721 catgtccaac aaagccttac tatccgaaga tataattttt ttattctcat catattcatt
     6781 atatatcaat tcaacattgg atatattaaa tgttgaatta ttttctacct ttgttgatgt
     6841 atctacttta tcaccttcat ctatatttat aatttctgat tttcctatat tcaatacttt
     6901 tgcatttccc tcatctattt ttatctcatt tttatcttct gtttgaatat caactgaatc
     6961 aggctgttca tctttttcac atgctgtcaa cataaaaata aatattgtta atataatcat
     7021 tagagaaatt gattttttta ctattttatt catattaagc acctcatata gtagtatttt
     7081 atagtagatt tttatacttt ttcataaatt ctttcaatgc ctgtgtaagt atttgatgtt
     7141 tttcaaaatt cgaattacta cataatcttt caaaatcatt ccatatttct ttatctatac
     7201 gtattcttgt tgtttttaat tcatctatgt gttcatcatc tatatgtaca tcttttttat
     7261 tagatacttt tttaggcttt atattgtaat cttttacttc taaataccaa tcatatactt
     7321 cacaaagctt atctaaatct tcttgagtta tatttatttt tctatctttc ttaggcttaa
     7381 cattttgttt tggctttgca ttttttaaac ctgtatcatt aaactcaatt tgttcaacac
     7441 ttttacttaa agccttttca ttagaaatct tttttttggt gcttactttt tttctattac
     7501 ttatagtatt ctttttactt ttttcatact ccttagcttt ctcttcttcc aagtttctat
     7561 tatccaaact tttattataa tttaattcgt cttttaatat gtatttacct ttttctgact
     7621 tatatccttt tttatttaaa aaagttctta aagtactttg actaatcttt agattttgag
     7681 ctatctctgc aaatttaaat cctttgcttt gaagttctaa aaatttgtca attctgtctt
     7741 cattgttatt cattaatatt cctccgtcaa catcttcttg tctttctttt catacttctt
     7801 attatattct tcttttctaa atctactcta tttgattcaa ttattttttg tagcatttta
     7861 ttataagtga aatcatctaa attattattt tttatataga gaaaagtttc ctcttcacat
     7921 ttaataaaac aaattgatag caaccatgcc atacccattt taacataata ttcttcactt
     7981 tgtatattat tacagtattc aaatatttct ttaatatgtt tatcatcaac ataatatatt
     8041 aaaaacataa ctaatgcaaa tctaatttcc catggattct ttgaataaac atatttttta
     8101 agtatatcat acatattttc tttatatttt ttcatagact ttaaatcaga acaaaatcca
     8161 tcgcaaactg accaattatc gattttaggt ataaatcttt ttacatagta taaagtttct
     8221 tcaaaattac tatttatatt gccaattacc ataccttgta gcctaacttc ttcatagtat
     8281 gtatcctctg ctacttctaa aaattccttc caatttcctt ggcttatttc ttttgataac
     8341 tttcttaaat taggaactct aatacctatt atattttctg ttccaggtat aagcttttta
     8401 ttaaaatctc tatattttat atcttgtata gacatcatat attctaaaag ttcattataa
     8461 tccaatttat tccattttcc cctatcaaac atcatttaat tgctccttaa ttcctttttt
     8521 cttattgtaa cataaaactc tacaaaaaaa gcttctaact attagaagct ttttcaaaaa
     8581 cataagaaac ttttcaattt taaatttaaa ctaaattaat gctaatctcc tacaccaaag
     8641 tctgtattat tgtataaaac ttctatattt ggattttcat ccaagaattt atacttacta
     8701 acaaaccatt tcactaactc ttcatctctt tttatactag aagaattatt tataaaaaca
     8761 ttcactgtcg ctttttcaaa agtatttagg ataatctcta tatctttatc tatcatctct
     8821 tttgtttgtc cttttattcc aaccataata caaggtgaat caaaatattc ttttacatct
     8881 tgtggagtct taaaatttgc atttttattt aaaatattat ttctaaaata attgtcaaag
     8941 gtttcaactc caattttaaa taccacagga atttcaaaat actctctcat ttccttaagc
     9001 ctatttttgt aagaccaatg actttctaaa aataattttt ttatattttt ctcttttata
     9061 atacacttta ttttttctaa agtatcctta ggtaattcaa aacagcttcc agaatttatg
     9121 acttcaagaa ctccatattt cccagttacg ttttttaaaa cctttagatt taatttattc
     9181 atctcttctt ctaaattaga attatcatct atataatcac aaaaagaaca ctttccccat
     9241 atacaaggaa atcctttaag cagaacaatt tctctttgat ttttatttgt tatctcacta
     9301 tatctatcca ttttctatct attttcctcc agtcaatgat acctttaaag tttttggaac
     9361 tgcatttaat cctaaattca ctaaaacaac tgctaacagc ttatctccat aatcagtaaa
     9421 tacttgggtt acaaacacac taaaaacatc tcccagtcct aacactttca agatttgcac
     9481 tatatatgat gaacctgaag aggtcatacc tccaaataaa aatgctgaaa ttattgaact
     9541 tattatagat gttggaatgg caaataaaag tactcctatt ggagttttta aaccccttaa
     9601 aaaattcttt ttaaatgcta ttccagcaag taatccggtt gatatttgaa ctggtgcaaa
     9661 atagagagaa tacacatcaa acgtaattcc acttattaaa cttccaaaca cacctgtaac
     9721 aacagcgtat ttaggtccta aaagtgaagc tatcattata gttcctattg aatctaagta
     9781 tacaggtaat cttaatccca tagctataaa agcgcctatt atatttatag aaataccaaa
     9841 agctataagt gtaatagttt ttacacatat ttttcttttc acactatatc actccttcca
     9901 cagagtctat aatgttttca taacctttaa aaagcctctt taaaaacatg tacataaatt
     9961 tcttctcatc aacttcattt agaacaattg cattaggatt ttttttatag aaattgaaag
    10021 aatccactat agactgaccc atagctattc catcttctac aacttctaca tatgattcaa
    10081 atcctttaca tatacttcta tctataaagt atgctactgc tagagggtca tttatcacac
    10141 agccaattat tccctcctgt tcccaatgga aatcaatata aaatctagtt atttcagtta
    10201 tatactttgc catcttctta tcaagtctat ttataaactc aataatatta ggagtaagta
    10261 caatttttct agttacatct aaacctacca tgtggatttt tttagataaa ttcttgtaaa
    10321 cataatctgc tccatgtggg tctacccaat aattaaactc tgctactgga gagcaatttc
    10381 catgaatcct aaatgctcct cccatagata caaactcatc gagattttca aatgctttct
    10441 tatctttaat aagtgcttta gctatatttg taagtggtgc aagtgctatt attgatactt
    10501 tttcatgatt atgtaaagta tttattataa aatccactgc tccatttttt gctttagctc
    10561 ctacaacctt ttgataaaag ttttctccaa taccatcttc tccatgtgta tcttgagctg
    10621 ttacaagttt tctttttagt ggtgcttctt ctcctatata tactggaata tttagtgaag
    10681 aacacatttg aagtgttttt agtgcatttt ctgcacctat atttgctgga acatttccac
    10741 aacatgtggt aattccaatt acttctagct ctggtgagtt taaagccaga agaattgcca
    10801 aagaatcatc aattcctggg tcacaatcaa ttattacttt tctcttttcc attttttatc
    10861 ctccttgatt tgtggtcgag gagaactaca catctttttt ccactttcaa aaaggtgtgt
    10921 ctaagggtat taattatata ctgccactat ataattcctt agttttttat acttggagtt
    10981 ctcgaacaag tacagatgta aaatcatatt tttaggctac ttactataga ctcttaaaaa
    11041 ctatagactg ttaaaaaatt tttaaacatc tgatttaata gctataaaat aaattttata
    11101 gcataaaaga atttattata ttttaatgta tcttattaaa gtttatttag aattatgaac
    11161 caactttaat aagatgtggc gcatttaaac cgattattat tatacaataa tatcttaaat
    11221 attacaatta ataacattaa tttattatcc ataaaaatat attaacttat attatttaat
    11281 aatttattga cagtattaaa attaaattat acaataacag tgtaaattaa cttaacaccg
    11341 ttaagcaaag aggtgattat atgagtatca aagaaagacg taaaaatgaa aaagaagaaa
    11401 tgaagaaaaa gattatggat gctagcatcg aaattataaa ccaacatgga tatgaaaatt
    11461 tatcaataag aaaaattgct acaaaaatag aatattctcc aactacaatt tatctttact
    11521 acaaagacaa agcagaaatt atcagcgata tgaccaataa actttataac acagtagaaa
    11581 gtaatgctat tgacattatg aataattgct catcactacc aatagataaa caaatcatag
    11641 aaattatgac tagctttata aaaactcttt ctagtgaacc agagatggca aaatctatca
    11701 tgcatagtag aatgaatatt atatttgcat ctgaaaatac aaacaatacg cctagcaata
    11761 atggtataaa aatgcttgat aaattccttt ccattggaat tgaacaagga atattcaaaa
    11821 aaaatataga taaaagctca tggatgctta taagtgcatt gttaggattc gttttatgtg
    11881 ttgttgaaaa taaactatat tctttacata atttttctca acttatagaa aatttcttag
    11941 atattttagt gggaggactt tacaatgaaa attcgcaaca aggctactaa taaaaagaaa
    12001 aaatatgtaa aaatatctat tgctctattc tttgtattta tatgcattat tattgtaatt
    12061 gtaagcaact taaagccaga aaacttggta gtaaaagata tagatattaa taatgttaaa
    12121 aatggcattt atactggaag tgctgataat aacttggtaa aagctactgt gtctgttgaa
    12181 gttaataatg gtaaaatcca aaatattaat atcttaaaac acgaccatct tttaggtaaa
    12241 cctgctgaaa aaattacaac aagtattatt aaacaacagt ctttagatgt ggatgctata
    12301 acttctgcaa cttatagcag taatactata cgaaaagctg tagaaaatgc tttaagaaag
    12361 ggagaataat tatgaatact attattatat attcaagtaa atatggttgt acaaaagact
    12421 gtgcaaatat actaaaaaat aaactatcag ataatgtcac tattgttgat attaataata
    12481 ataataataa aattgagtta tcaaaatttg ataaaattat tattggaagt tctatatatg
    12541 ttggttcagt ttcaaaaaaa atacaagtgc tatgtaatga taatgtagag ttattaaaca
    12601 aaaaacaagt tggcattttc ctttgctgtg gcttttctga acaagcagat aaatatttaa
    12661 aatcaaattt tccatcatcc ttattagaaa gtgcaaatgc tattggaatt tttggtagtg
    12721 aagctcgatt agaaaaaatg aaatttttag ataaacttat tatgaaggct gtttcaaaag
    12781 gtaattatga cagttttaga atatcacagg ataatataga taatttttta ataaatctta
    12841 atagctaaaa taaagctaat tatttctatg ataaattctt ttatttataa gtaatattgc
    12901 tcaaaatagt ctttaaactt tatcaaaatt ttatttttct tagtgaaatc taatgaaact
    12961 atcacttaat atatgcttta ttaatcataa caaaaagaga gtgtctaatg aactaaaaaa
    13021 ttcattagac actctctttt tgtatgaaat caaatatatt tttatcattt caaaagtgaa
    13081 aaatacactt gtctctatgt ttggcacatt ctctttttat aatataatat ctaatttaaa
    13141 acctatttca aatattgaca tatacttaaa ctatagactt acaaaatcaa ttcatattag
    13201 cttaaaatat aatctttgtt attcaaattc ctatacacaa ccattattta ctttgatact
    13261 ttaaaacatc ttccctattt tttattaaat tttataataa tagtcctatt aacgcaaata
    13321 ctataaaaag agtttaactt tatccattaa ataaatttaa attaaaacat atagaaatgg
    13381 aaggagaaca atttatgata aaactaatcg gtatattgat agttataatt ggatttatac
    13441 ttaaaattga cacattattt actgtattat tagcaggagt ggcaacagga attgttgcag
    13501 gattagattt taatcaaatt tttacaattt taggagattc ttttgtctca aacagaggtg
    13561 tatcattatt catattaaca ttgccagtaa tcggtgtatt agaaagatat ggtttaaaac
    13621 aaagagctgt ttctttaata gaaaaattaa aaagacttac aacaggaaaa gtacttacta
    13681 tatacatgat agctagacaa atagctggtg ctctatcaat aagaatgagt ggacatcctc
    13741 aatttgtaag accacttgtt aatcctatgg cacaagctgc tggtttaagt aatagtgatg
    13801 agctaaaaga atcggatgaa gaagccataa aggcattatc tgctgcatca gaaaactatg
    13861 gaaattttta tggtcaaaac ttatttgcag gtagtagtgg agtattactt atagcatcta
    13921 ctctgactca atttggatac aatgtaactg gacttaatat tgtaaaggct agtactatta
    13981 tggcagtaat agcacttgta gtggctacaa tccagtttac cttatatgat aaaaaattaa
    14041 acaaaagtca taaaaaatag ttttatttag gaggaataaa aatgcaaaat tttataaaca
    14101 tatcattaga aatcttttac gcactaatgg gatttttaat gattgttata gcatataagt
    14161 cttttactac cataaataac aataagaaat atggtacatc attattttgg atacttatat
    14221 cactgccatt catatttggt agattgattc cagccaatat aataggaata atattaatat
    14281 tatctagttt attaacactt tctaaacaag ttgtatttgc aaagtatgaa gaaccagatg
    14341 aaaattttgg aaaagaacaa gcagacaaac ttaaaaataa aatatttata ccatcattaa
    14401 tattagcatt tgctgcagta gtagtagcta tgagcctttc taattttgat aattcttctc
    14461 aatttgcaat aggtgtgggt tcaataattg cattaatatc agcccttata ataactaagg
    14521 caaaatctgc tacatcagtt caagatggct caagattact tcaacaaatg ggacctgcta
    14581 gtatgttacc acaactttta gttgcactgg gagctttatt tactcaagcc ggagtcggtg
    14641 aagttatttc aaccatgata tctggtgtag tccctgctga tagcagatta tttggtgtta
    14701 tagcttatgt attaggtatg gttatattta ccatgataat gggaaatgca tttgccgcat
    14761 tttcagtcat aacggcagga attggtattc cttttgtatt gtctcaaggt ggaaatcctg
    14821 ctataattgg agcattagca ttaacagcag gatattgtgg aactctactt actccaatgg
    14881 ctgctaattt taatatagtt ccagcagcct tgctagaatg taaaaatgat tatattgtta
    14941 taaaatatca agctccagtt gctttggtat taattatagc ccacatatta gtaatgtact
    15001 ttttaggatt ttaatataat taggaggtaa aataatgaaa attttgttaa caggatttga
    15061 cccatttggt ggagaaccaa taaacccagc tcaagaagca gttgaaaggg tcaataataa
    15121 tataaacggt gcagaaatca taaagataac tataccaaca gttatgacaa aatctgttga
    15181 agctatagat aaagctattc aagagcacaa tccagatata gtaatatcag ttggacaagc
    15241 tggaggtaga tttgacatta cacctgagag agttgctata aatatagatg attttagaat
    15301 aaaagataat gagggtaacc aggttataga tactattata aaagaagatg gtgaacctgc
    15361 atatttttca aaattgcctg taaaagctat ggtaaaacat atgaatgaaa ataaaattcc
    15421 agctagtgtt tcaaacactg caggaacatt tgtttgcaat catgtgatgt atggaattct
    15481 ttatatgata gacaagaaat atcctaatat aaggggtgga tttatacaca tcccatatac
    15541 aacttcacaa gtaatagata aaaaaaatac accattcatg tctttagaag aaatagtaaa
    15601 aggcttagaa ttagctatag aagcttgtat tatatataaa gaagatgtaa aagaaattgg
    15661 tggtgaaatt tcataataaa ttttttagta tttttataaa ttaaaaatac taactattaa
    15721 tttaaatata gactgtgagt agattattca aacaatttta ctcacagtct ttttatggta
    15781 tctacagata caaaattaag gtctattcct ctctccccac tcacacattg catctaaaat
    15841 tggaatcaga gattttcccc tttctgatag actatattct accttaggag gtatttgagg
    15901 atattcagta cgaataatca agttatctgc ttctaattct tttaatgata aactcaaagt
    15961 tttataggag atagtattta tataacgttt taatgcatta tatcttacaa cttcaaattc
    16021 tacaagacag taaagaatta tcatcttata tttaccacta atcagtgaaa gtgtataact
    16081 aaatcctgtt tcacttaaat ctgtatttga tatacagtct atactcatat attacactct
    16141 ccttttggtt agtattgaac aaaaatgtgc gtacttgttt ttagaataat ttataataaa
    16201 attatagtgt aagaataatt gaaagtcaat tttataatag gaggtgtatt tactaaaaaa
    16261 aagttatttt aaataggatt ctaagattag acatttctat ataaattgat aatattcatg
    16321 aaaatcctat tttataaaaa ataatatctc ttagaaaatc tattaaataa aattcatggg
    16381 aggaaattta taatgaaagt tttattgata aacggtagcc caaaccaata tggatgtact
    16441 tatacagcac taaatgagat tactaaagta ttgagtaaac acgatattaa aacagaaata
    16501 ctgtatttag gtaaagaaac aatcacagga tgtattagtt gcgcatcctg ttttgaaact
    16561 ggaaaatgta ttcgaaatga taaagttaat gaattgattg aagatctgga taatattgat
    16621 ggtattatta ttggttctcc tgtttacttt tctagtgcaa ctggtcaatt aacttctttt
    16681 cttgaccgtc tatttttcat agctggaagt cgcatggcta caaaattagg tgcttcagtt
    16741 gtgtcatgta gacgtggtgg tgcaagtgca acatttgacc aactaaataa atatttttca
    16801 atcagcaata tgcctattgt atcatcacag tattggaatc aggtacatgg ctttactcca
    16861 gaggatgtta tgaaggatga agaaggatta cagactatgc gaactctagg agaaaatatg
    16921 gcatggcttc ttaagtgtat atcagcaggt aaaaaaactg gaattaaaga accacaatat
    16981 gaagaaataa ttatgactaa ttttataaaa taaataaaaa atgtactaat attaattaaa
    17041 acaaaacact aatttataaa taaaataagc ataaatgtag gataaaaagc actaaaacag
    17101 aggtattgaa ttttatatca aacctctgtt ttattattac attaataacc aaaccatgta
    17161 taacatcata tatttctaaa taaattataa tattatattc taatgttatt gaaattacaa
    17221 taactgaatt tctgctcata aatttaaata ttctacaaat ctcatctaaa taacaatttt
    17281 aatagaccta aaacaatcct catattgttc attttgtatt aaaaatatca cttacaatca
    17341 aaaaccattt attaagtata ttcctaagat atacaatata tatacatgga ttggatagta
    17401 taaataaaat aaatacttca tactcttccc tttttctcca ttgtaaagta acattaaagg
    17461 tagagaagca atcatcatcc attggtaatt ttgtaaaata gaatttttta aactataatc
    17521 tccatttgac attgttgtaa atataaatat acacattaaa gcataacaaa tactcaaact
    17581 gaactttttc cctctacaca aataaaaaat aatcccaagt ataatccaaa ttggtccacc
    17641 ttctactaaa aatggtactg gtaccaaaat cataaataaa gcaataactc tgttagtgac
    17701 tttaaaaagt aaagctactg taaatattcc taaaattata ggaatgatta tcatcaataa
    17761 tcctataata aaatatttaa tttctttctc ttttctaaat ttctttatga actctattgc
    17821 ttgtaaataa atcgctatta aaaataatgt tgaaaaaatg ttatttataa ttatagctcc
    17881 ttctggatgt gaaaatacat tattaattat ttgatttcct atagccataa taacagacca
    17941 aatatacaat ctacttatat atttttttct gttactagtg tgataaaatc cttcaacagc
    18001 cataaatata aacagtggtg ctgatattct gcctacccaa tgaaaccaaa ttggtatttg
    18061 cattgtttgt ggcataaatg ctgctatatg gtcaaatgtc ataaatatta atgctaaaat
    18121 cttaatagta aacccactta tacctttttt aaaaaaatcc atatcatatc tcctaatttt
    18181 gttttatctc tataataatt tttgttccta ttccaatttc actacttaca taaattttac
    18241 ctttatgaat ctctattatt tctttagaaa tagccatacc aagacctgaa cctttatgca
    18301 tctcacctgt attagtacct ctataatatt ttttaaaaat atgtttcaaa tcacttttgc
    18361 ttattccaat tccattatct tttattataa tttctatatt atcttttttt acaatttcta
    18421 cacttatcaa agttccttcg ctattatgaa ctattgaatt aaatataaga ttggttatag
    18481 cccttctgaa taagattgaa tctacatata cttcaattaa atcttcatta ctttcaaatt
    18541 caatattcct atttttatat ctattatcat ttaaaatatc aattattgta tttctgacta
    18601 aactgactaa atttattttt ttcttgtcca gaactatagt attatttttt aatcgagtag
    18661 ataaatttaa atcatcaact aaatctttta tatacttaga cttattatat ataatttctg
    18721 tatactctcg tatttcatct tcttcaaact cataatcttt gtcattaatt atctctgcat
    18781 acccttgtat agatgctaga ggtgtcttta tatcatgtgt tatatttgcc agccattcct
    18841 ctctcaactc ttcattttct tttctctcaa cttcatttac tcttaaagta tcagctaaca
    18901 tatttatatt tttaaaaact tcttcatata tacctctgtc cttaagatat aaactataat
    18961 ttccattagc catagtctca atactccata gtacgcttga aattggtttt gtaagttttc
    19021 tactaaataa atatcctacg cctaaagcta ccacgctatc aattactagt gttataatga
    19081 taactttatt taaagttttt ttaatgttat tttgagaata ggttaaaatt attcttgata
    19141 tatctctact aggtatacct actattatgt ttagatgttt tttgtttaaa tatttttcta
    19201 gtacaaaatt aacatacttt gtttctatat acttgtaatt attcaccatt tgaaagggtg
    19261 tatatttttt gggagtatcc tttggcacat ttactccata aacttcctct cctaaatcat
    19321 ttaaaacctg tatccaggaa tttgattttt ctaatttttc agctccaatc ttagatagtt
    19381 taaattctcc atctttttca tataaatctt tttcaaaaga acgtgcaaat tcttcaggat
    19441 tatttccaga atctacaact ttaaaaaaac tattagttga aatcacatac aatatagata
    19501 tgatatttat aattactact gatatagcta caaacacaat tgtaaatata aaatttctag
    19561 taatcttcca cttcatattc taatcttcct ttactgctaa tttatatcca agacctttta
    19621 ttgtcagaat atattcaggc tttgaaggat tatcctctaa tttttcccta atttttctta
    19681 tatgaaccat tatagtattg tcaaatccaa cataatcttc tccccacact ttatcccata
    19741 gtttttcttt acttataatt tgattttgat tatgtgcaaa taaagatagg atttttagtt
    19801 cctttgcaga cagtcctata gattttccat cttttataag ttctgcccta ctttcattta
    19861 atatatatgg gccaaatgat ataatttttt cttcattatt atttagattt ttactttcgt
    19921 tttgagcatc actaaaataa cttaatcttc tcaaatgcgc tttaactcta aaagccactt
    19981 ccttagggct aaaaggtttt gtaacataat catctcctcc aatcgctagt ccaagtaact
    20041 tatctaattc ttcggtttta gctgataaaa atattattgg agaattggag gtttttctta
    20101 tttctttaca aatatcataa ccttcgccat ctggtaacat aatatccaat acaatcaaat
    20161 caggattaat acttttaaat aattctaatc cttctttttt agtttttgca gtatatatat
    20221 tattaaggcc ctcttttttt aggacagttt ctaaaagctt caatatttct acttcatcat
    20281 ctattattaa aacacgattt tccataaaca acctcctgta ataatttaca tatatattag
    20341 catatatttt atctaaaatt catttatctt atttttaatt aaactaaaat ataaataaaa
    20401 ttactattat ttttagttaa aaccttttgc ttgatgtaaa attttcatat gttattttag
    20461 ttaacactat cattaatata attgagattg ctatagaaaa aactatcaaa atacttggta
    20521 cattatactt aaccatttgt tggctcatca taatccaatt tggagtcact gacataaatt
    20581 ttatagcttt atctggtata aaacctacac taaagtatgg aattgccatt atcaatccac
    20641 ctagtataaa tggaattaca gccgatttaa atatagatga aagtgttaag cctatttgtg
    20701 caaatataaa agaagcaaaa atagtagtca atataacaat tatatacatt tgtattactg
    20761 taagatttaa attactattt ccaaagtacc ataagttatg aatcggttca tttaaatttt
    20821 taaaatcaaa aaaactaaaa cctctaattc ctactaacaa tataaaagaa atattaataa
    20881 tagataaaat taatccatta gctactagtt ttgataatac tactgatttt tttccctttt
    20941 tagttgtatt tattaaacct gtcatattag aatcatattc tttaatgtaa tttccagaag
    21001 ttattataaa cattatcaaa cctattagag gtattgagcc ttctatcttg taaaatatat
    21061 ctgcataagt atctaaaaca tcaaactttc taatcagaaa aaatccaaaa aatattaaaa
    21121 acagccaaat tatccaaaca ctctttgatt ttaatatttt tatcatttca gctttaacta
    21181 aatacatcat ttctactaca cctcccttgc atcttccaaa tcaaaataaa acatgtatac
    21241 atcttcgaat tttgcttgaa ctggttgtat attatcccca cttggaatat cctctgaaac
    21301 aattctaagt actactgaat caatatcact ttgaatactt acaaccttat atttagattg
    21361 tatatcatca acttcacatt catttttaac ttgaacatta tatactttat tttccatgtg
    21421 acttaatatg tctttgtgtg ttcctttcat tagaagtttg ccatctttaa tcattattgt
    21481 ttcctttgca actgattcta tatcggaaat tatatgagta gaaagtatta ttatcttatc
    21541 tctagacatt tttgctatta aattcctaaa tctagctctt tcttgtgggt caagacctgc
    21601 tgttggttca tcaaggacta ttatcttagg gtcgtttagt aaaacttgtg ctatcccaac
    21661 tctcctcttc atacctcctg aaaattttcc tatagactta tttctcacat cataaagacc
    21721 tactaaatct aataattctc ctatcttact atttaaatat tttcccctta aaccttttaa
    21781 agctccaata tactgtaaaa attcttttgc tgtaaaattt ttataaactc caaattcttg
    21841 tggcaaatat cctaaaatat ttctataatc agcatccatt ttaaatatat cctcaccatt
    21901 ataaagtatc tgaccttcat caggttttat caacgttgtt atttgtttca ttaatgtaga
    21961 ttttcctgct ccatttggtc ctagaagacc ataaactcca ttttccagag ttactgtaat
    22021 cttgtcgttt gctacttttt taccatatat ctttgaaaga ttttctattt ctaatttatt
    22081 catcttaact ccccttttat ctacttatat aatctgattt tattactaaa aaacataatg
    22141 acatagcacc tatcaatatt gccatcaaaa gtcctattgc tatattgaca atgtctatat
    22201 tcactttaaa tatcataata tcacctagat aatttcctat tattttatta aaaccaaata
    22261 tcaaatcata ataatttaca tttgatacaa ctgaaagtaa ttttttaggt aatatatgga
    22321 ttaccgacat tatcttagtt atagatataa taaatataaa aaaacttatt gaagatattg
    22381 aagacttact tataaaagac ccaaatatgg ctatcatggc ccatccttca aacatcaaaa
    22441 gctctatgcc tatatttaat attacatact ttgttatact taaattagtt ttcaatgcaa
    22501 ctacattgtc tactattcta aaagattcta agtctccatt aacaggtact ccttgttgca
    22561 tacaagttat aataaatatt cccattaaat aaacagaata aattactaat ggaactaata
    22621 aagcaacaaa tattttagac tttaatgctt ttttcttctc ttttgatgat agtatgatat
    22681 cttttaccga agtaacgact tcatctacgt aaatattgga tataattata gatataatga
    22741 ttaacattcc tacatttata agtgaatttg taactctata gttaaatact ttccaaaaac
    22801 taatatcctt atattcattc acacttagat tatcaagttt tttctttgac attaattcaa
    22861 tttttttaga aaattcacct gtatctgttt gatttgaaaa ctgctttaaa acattaatct
    22921 tattttgaaa tttctcatta cctatgtcga taccacttct cgtatcttct atacgtcctt
    22981 tttctttatc tatataataa ctttctgttt ctaaaacagg cgtaataaag atactaatca
    23041 ttagtattaa cactaatatc cctagagata taaacatact cttagattta gccaattttt
    23101 tatttcccaa tttaccaata tatttctcct cctaaatcat tttaattaca catatatatt
//
LOCUS       DAEMIK010000053        23068 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEMIK010000053 DAEMIK010000000
VERSION     DAEMIK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.053"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(283..1374)
                     /locus_tag="KPK87_001773"
     CDS             complement(283..1374)
                     /locus_tag="KPK87_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537925.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEVKQEMIVMKEDMSEVRQEINIMKEDISEVRQ
                     EMTVMKEDMSEVKQEINVLKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1604..2689)
                     /locus_tag="KPK87_001774"
     CDS             complement(1604..2689)
                     /locus_tag="KPK87_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG2537926.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2725..4038)
                     /locus_tag="KPK87_001775"
     CDS             complement(2725..4038)
                     /locus_tag="KPK87_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2537927.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAHVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKRAIVKEMKEVACEFWKF"
     gene            complement(4039..6330)
                     /locus_tag="KPK87_001776"
     CDS             complement(4039..6330)
                     /locus_tag="KPK87_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2537928.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMVMKKLNQGGK"
     gene            complement(6320..7114)
                     /locus_tag="KPK87_001777"
     CDS             complement(6320..7114)
                     /locus_tag="KPK87_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2537929.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7153..7599)
                     /locus_tag="KPK87_001778"
     CDS             complement(7153..7599)
                     /locus_tag="KPK87_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG2537930.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7671..9077)
                     /locus_tag="KPK87_001779"
     CDS             complement(7671..9077)
                     /locus_tag="KPK87_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2537931.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9095..10465)
                     /gene="hydA"
                     /locus_tag="KPK87_001780"
     CDS             complement(9095..10465)
                     /gene="hydA"
                     /locus_tag="KPK87_001780"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG2537932.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10598..11785)
                     /locus_tag="KPK87_001781"
     CDS             complement(10598..11785)
                     /locus_tag="KPK87_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG2537933.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11912..13126)
                     /gene="dpaL"
                     /locus_tag="KPK87_001782"
     CDS             complement(11912..13126)
                     /gene="dpaL"
                     /locus_tag="KPK87_001782"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG2537934.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13489..14160)
                     /locus_tag="KPK87_001783"
     CDS             complement(13489..14160)
                     /locus_tag="KPK87_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2537935.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14243..16459)
                     /locus_tag="KPK87_001784"
     CDS             complement(14243..16459)
                     /locus_tag="KPK87_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2537936.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETTEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16462..16932)
                     /locus_tag="KPK87_001785"
     CDS             complement(16462..16932)
                     /locus_tag="KPK87_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG2537937.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(16994..18388)
                     /locus_tag="KPK87_001786"
     CDS             complement(16994..18388)
                     /locus_tag="KPK87_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2537938.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18461..19822)
                     /locus_tag="KPK87_001787"
     CDS             complement(18461..19822)
                     /locus_tag="KPK87_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG2537939.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20119..21441)
                     /locus_tag="KPK87_001788"
     CDS             complement(20119..21441)
                     /locus_tag="KPK87_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG2537940.1"
                     /translation="MFDIDGRPPILKCLQLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGTFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIIKFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(21796..22836)
                     /locus_tag="KPK87_001789"
     CDS             complement(21796..22836)
                     /locus_tag="KPK87_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2537941.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         6866 a         4658 c         2535 g         9009 t
ORIGIN      
        1 caatatttgc atacatttgt atagtataaa atttttatgt tctcattgat aaaattttac
       61 ttatttcaag cgttctatta gattttatca tctaaatttt aggttttatt tttataaatt
      121 aattgtattt acataaaaaa aaagacctaa aaacaaattt ttaacggtct tttctcaatt
      181 ttttattttc atttctaaaa tttataataa atagtaagaa cactataaat tcttttatat
      241 aaaattctta gtttgtggat gataaattag tgaattaaaa tactacttta ctgattttag
      301 tttgacaata tcactccaat ttttactagt ggctaactct actaaattta agtctcttct
      361 tatttcagct atatcctctt ttatagaatt agtgtcacct tttatatatg ctatgttatg
      421 ttctatatta tcatgagtag ctttatttat ttcagaatta tgtaataaac ttcctaaaat
      481 attattctgt tctatctgta tctctttcat tgatttatct tcacattcta gactatcaat
      541 cttaatttca attccatata atttttcatt tgctatggat acctcgtcct taattccatt
      601 catatcttca ttaatgttat ttattttatt ttgcataaag tttacttctg atttaacttc
      661 ggaaacatct tctttcatta cagttatttc ttgtcttact tctgacatgt cttttttcat
      721 tacatttatc tcttgtttta cctctgatgt atcttctttc attacagtca tttcttgtct
      781 tacttcagac atatcttctt tcattatatt tatctcttgt cttacttcgg acatatcttc
      841 ttttaataca tttatctcct gttttacttc cgacatatct tctttcatta cagtcatttc
      901 ttgtcttact tcggatatat cctctttcat tatatttatc tcttgtctta cctctgacat
      961 atcttctttc attacagtca tttcctgttt tacttccgac atatcttctt ttaatacatt
     1021 tatctcctgt tttacttccg acatatcttc tttcattaca gtcatttctt gtcttacttc
     1081 ggatatatcc tctttcatta tatttatctc ttgtcttacc tctgacatat cttctttcat
     1141 tacaatcatt tcttgtttta cttccgacat atcttctttt aatacattta tttcctgttt
     1201 tacttccgac atatcttctt tcattacagt catttcttgt tttacttccg acatatcttc
     1261 ttttaacaca tttatttctt gttttacttc cgacatatca ttttttatgc taactacatc
     1321 ttcatttata gtatataata actctaaaat cttctcttgc acttgcatca ccatccctat
     1381 taattattat aacattttac acaaatgcta tacaatgtta acatatatat cagaattatt
     1441 aaaattttaa ttaaacaaaa ttgattcact ttattacttc aaaactaatt aatatctaaa
     1501 ataactacaa ataaacaaaa ataaggtcaa caacaaaaaa ggcttatctc tttgagatag
     1561 cctttcttat ttttatttaa aactttgttt tttataaaat aatctataca ttcagctctg
     1621 aacatgaaat ttttcttcca agtgcttttg gtttacaaac tgatatacaa agtccacaat
     1681 atctacactc ttcttcattc aattccatct tatcattcaa ttttctcgct tgatatgcac
     1741 aaactctctc gcacatctta catttagtac atttttcttc atcataagta aactcaaagc
     1801 taagtctttc attaacatct cccatatgat tatgtactat accacaagca ttttctatag
     1861 tattatatcc taatctatta agattagatt ttaaatcatc atgtatttta gatattactt
     1921 gtgcgccttt taaaattact actgaacata taccaacaaa gtcagcacct gccataatca
     1981 tttctaaggc atcatctcca cttacacatc cacctaagcc aattattggt atattaactt
     2041 gtttttttat ttcatatatt ctctgtaaag ttattggctt gatagtttct ccacttaaat
     2101 atcctattcc atttccacct aataaaggtt gccctgtttc tatgtctatt ctaaatgctg
     2161 gtcctacaga gtcacaagca gttattgcat ctgctcctgc ttcttctaat tttttagcaa
     2221 aatcgcctat ctcatctatc ataggaggaa gtttaactat tacaggtata tttactcttt
     2281 cctttgcatc tttaagcatt ggtatcaaat ctctatagtc ataagatact aattcaataa
     2341 agtcagctcc tgcatttgca actttctcta ctaattcgct actttcttca attgtatgac
     2401 ctattgaagc tatacagaca gtcccatctc ttttcatttg tggtatttca aaatctatcc
     2461 attgctctgg ctcaaaatct gtccattttt cattatttaa aagagcatta gctgtatttc
     2521 taaccatatg tggtgctgga tttatagctc tttcttttct tatagtctta gtcacaactg
     2581 cccctgctcc tgcatcactt aatatcttgc atccagctgc actatatgtt aatggtcctg
     2641 aacctattat aagtggtgat tttaattctt tacctaaaaa attatacatt atctgcttcc
     2701 ccttttcata tatatttaca ttcgttaaaa tttccaaaat tcacaagcaa cttctttcat
     2761 ttctttaaca atagctcttt tatctaaatc aactaactgt ttttctttta aaatatgtct
     2821 accatctatg tatacatttg atataaattc ttttttaccc tgaactacta tctggtcaaa
     2881 tatattatca agagtcacag gagttttaaa ttcatcttcc ataactatta tatcagcctt
     2941 attaccaact gctattttac cagaattttg aagtcctaat acatgagcac catgctctgt
     3001 tgccattcta aatactaaat ttgctggcat tactgatgca tcttcttcaa ctgatttatg
     3061 cattaaaaat gcacctctca taacttcaaa aaaatcattt atatatccat cagtacccag
     3121 tgccactttt atgccttttt taatcatttt tggtactgga gaaaaacctc cacctacttc
     3181 acagttgcta agaggcatat gaaccacttt tacacctttt tcttttaata tatctatttc
     3241 ttcatcattt acttttacac actgagatgc taatacagtt tcatccaata tatctaaatc
     3301 attatagtag tcaactgctt ttttgccaaa atgtttttca gcatagtttg gctcatatat
     3361 actctcacat aaatgaaatt gcattggtgc atttaatttt tttgcatctt ctttagcttt
     3421 cttaataaat ctatcagaac atgtgaagct tgtatgtgta cacattattc catttattaa
     3481 tttactattt tctctactcc actttattaa attagaattt tcatctaaac atctaagtcc
     3541 attttcaaag cttattcttt cacaactttc taaggataaa atcgctttca tgcctatttc
     3601 ttctagtatc tttccttgct caattaaagt tccttcttct gtgtttggag cttctagggt
     3661 atcacaaaaa gctaccacac cagattctat aagttctatt gctgatgctt tagttgttgc
     3721 cttcacttct ttcaatccaa ttctattttc tataaatggc caccaatagt catttaagaa
     3781 accttcaaag tcggtaaaat gtacatttgc gggtattccc ctagaaagaa ctccatattg
     3841 atgcatatga ccattgacga atcctggcat tattatagca tctgatttat ctattatttc
     3901 ttcaatattt ttatatttta tttcaacttc ttcatttggc aatatatctt ttataatgtc
     3961 attttctact acaacagcag aatttgtata tatttcatca gcagaagata ttaagtattt
     4021 acttttaata gcaatcattt atttacctcc ctgatttaat tttttcatga ccatataaac
     4081 tttctcagaa gttataggaa gttctcttat acgaactcct gtggcattgt atattgcatt
     4141 tgctattgct ggtgcaggtg tatgaataac tatttctcct aaagatttag ctccatatgg
     4201 acctgttggc tcataattat ctacaaaatc aacaattatg tttcctatat ctttttttgt
     4261 tggaactttg tattgtaata gattagaagt ttgaagcttt ccatcagaac catgtctagg
     4321 gtcttcatac attgcaagcc ctataccttg agttacacca ccttcaactt gtattctagc
     4381 aagtgcagga ttcataactg taccacaatc tgtagaacaa gcataattta ttactttaaa
     4441 ctcccctgtt gttttatcta actctatttc agcaaatcct gctaagaatg gtttagcact
     4501 ctcttgtata ccataagatt cagatgaata taaaacttct tgatttccac caccaacaga
     4561 ttcttttcct aattcactta acaaaacaaa cttctctaca tcttcactat aacaaactct
     4621 gtcttcatat agtattaaat catctattgg taaatcgagt ttcttctgtg ctacttttaa
     4681 tatcttttgt tttaaacttt cagcagattt tattgtagca tttccagtta cataagtagt
     4741 acaagaagcg tatgcaccag tatcatatgg acacatatct gtatctcctg tataaacgga
     4801 aattctatca atagatgtat tcaatgcttt tgcagctatt tgtgcaagta ttgtgtcaga
     4861 accagttcct aagtctgaag acccagaaaa caacttataa gttccatctt catccatcct
     4921 catatttact attgccatgt ctatgttggc tataccagaa ccatgagttc ccacagacat
     4981 tccaacagcc ctcaccttat tgtttccaat atccctaaca gggtactttt catcccatcc
     5041 aataagtttt ttacctctaa ttatacattc atcaagtgca caacttctta ttggaaaatt
     5101 cattattccg ccttcagttt gtttttttat aatattcttt aactttattt ctgttgggtc
     5161 catattaagt ttatgcgcta attcatcaat tgcacaatca agtgcaaatg taccttgtgt
     5221 tgctccatat cctcttaatg ctcctcctgg aactaaatta gtatatacag ttttcccatc
     5281 aaattttatt gcaggtacat tattatatgt tggaagtaca ttatgacctg attcagagca
     5341 aactgaaggt ccattatcgc cataagcgcc tgtattgttc aatgctttca tatctatagc
     5401 ttttatgttt ccttctttat ctgccccaat ttttatatca aaaaacatct gatgtctagt
     5461 attagtcatt gcaaaagttt cttctctagt ataaattagt tttgctggtc tttttgtcat
     5521 ccaagttaca aatgatgcat atatctctgt tacagctatg ttttttccac caaaacctcc
     5581 acctattctt ggttttatta ctctcacttt tgaaagaggt attcccagtg ctttagaaac
     5641 ctgtcttctc atatgataag ctgattggtt tgcagaagtt actactaatc tgccattcgt
     5701 atctatatac gtataagctc tatgagtttc catcatacaa tgggcttgtg attgagtcat
     5761 ataagaatgt tctactatta catcactttc tttaaactct ttttctacat cccctttacc
     5821 cattttttct ctcgatacaa tattttttgt agcatccaat ccaaaatcaa atggacaaaa
     5881 caaatcatct tcttcatgga ttaaaatttt atttccttcc gattctcttg catccaatat
     5941 aggagtcatt acttcatatt caacttttat aagtttcata gctttaatag cagccctttc
     6001 atcttcagcg gctattatag caacttcatc tcctacatat ctaactacgt cttcaagtat
     6061 taattggtca tgaggtgatg cttctggata tgactctcca accattgaat atctataatt
     6121 tggaacatct ttgtaagtaa atatgtttac tacacctttt acctttaatg cattttttgt
     6181 atctatgttt tttatctttg caaaagcatg tggacttcta agaagcttaa ttgttaaaac
     6241 atctttatga aaaattaaat cttctgtata tattggtttt ccagtaacaa tggcttcact
     6301 atctattttt cttacctctt tatttaccat cacactcacc tccaatacta ttatacattc
     6361 tcactactaa ggcttttgcc atatcttttc tgtattcttt acttcctctt atattacttc
     6421 caaatgaaag ttcttcactt gatacttcac cagctttttc tatatcattt tctaaactta
     6481 atatttcact tgctttatag gcaatttttg ctcttcttgg tctagcgcct atcgcaattt
     6541 tataaatact tccttctttt aacattgctc catttaatac tgcaaaatca cctgtacatt
     6601 ttcttataga atcaaataca cctatagcat cttttttagg taatataact tctattaata
     6661 tatctttttc aagctcattt tctaaaaaat cagctaagtt cataacccct tttttatata
     6721 acttaacctt tgcatctacc actagtaatg aaggtattaa atctgaaaat ccatacttag
     6781 caaatacgct agctcctact ctagctccac ttctaaattg aactcctact atattagaaa
     6841 cagcgtttga aattacccca ttacagtaat ttttaataac cttatttaat tctaaagttc
     6901 ttaaacttgt atctgctcca attaatatgc tatcatcagt ttcatttata taatctaaag
     6961 ctaagtcttt taaatctatt gctgaaccaa tatttttact acctagtttt aaaaatccac
     7021 atcctccaag tattatgttg ttttttcttg atattagcag ttcataagct tcatctaaac
     7081 tttttggaac tacatattcc tttatagtta tcatactctc tccccatatc aacttaattt
     7141 tcacttaaaa gtctaagtgt ttaaatattt atgtattgct cttaattgac ctttatatcc
     7201 tgtacatcta caaaggttac catttacata atctataact tcttcttctg ttggattttg
     7261 aagctctctt ttcattgcca agaccatcat tataaatcca ggagcacaaa atccacactg
     7321 gtcagcccct tcgtctgcaa gaaaatctcc aaacttcttt gcttcatcca agacaccctc
     7381 aagtgttgta atatgctgac cttctactct agcagtaaga taattacatg aaagcacggc
     7441 cttgtcattt accatcactg tacataaccc acatgagcct gtatcacacc cttttttaac
     7501 acttacatat ccattttctc ttaatgtatc tgctaaaaaa tcatctggtc ttatacttaa
     7561 ttctttttct ttattattta tggtacattt gataatcaaa taaatcacct caaaactcat
     7621 atttttaagc taaagtttta ctatactttt ttatgaataa attacattta ttaataaagg
     7681 ctattccatt tatgaccttc cattctattt tttatagttc ctcttacaca aagtctctca
     7741 gctgtatctt gagcatcttt taatgctttc tcttcattag ctgtcaatat ttttgaatct
     7801 tccattatta tatttccatc tactataaca ctctctatat ttttcatact agaagaatat
     7861 actaatgtag ataccggatt atgcataggt atcgcttttg gtgaaagcat tggattaaat
     7921 attaataagt ctgctttttt acctatctcc aatgaaccta tctcatcttc cattccaata
     7981 gctcttgctc catctatagt tgccaactca agaacttttt ctgctgacat tgctaatggg
     8041 tcacacttat taactttatg ttgaagtgct gttaatttca taagttcaag catatcttga
     8101 gagttattac tagcggctcc atctactcct aaactaactg ttattccttt tttaagcatt
     8161 tctggcactg gtgcaacacc tgaagataag tacatattac ttgctgtatt atgagacact
     8221 ttcatatcgt attttttagt aagttccata tctttttcag ttagatacac acaatgtacc
     8281 ataagcacat ttggtccaag tattcctaat ttttctaaaa catctatgtc atattgccca
     8341 tgcaattctt ttgctgcttc tctatcaaaa ggagtctctg atatatgtac tgtaaataaa
     8401 gcatcatcat attcttttac tactctccat agcatttcaa gcatttcttg agaatttgac
     8461 catattgctg caggagctac tcctattttg attcttccat tttctgtatt atggtgtttt
     8521 tcaaacaatc ttcttacatc tttttcaacc gtttctacat cttgcattat tccaggatga
     8581 actccaaatt gagctcctgt attcatacat cctcttccaa gtattcctct tattcctaat
     8641 tctttataag catctattat tccatcacaa agacctggct tactatgagg atacatgtag
     8701 tcaaccattg ttgttatacc gcttctaagt ccttcaatac atccaagcat tgcagcatca
     8761 taagtatctt ttggctctaa atagttagct gctggaaatg tcatagtttc taaccagtct
     8821 ttaagtacca tatcatctcc caatcctttt aataaagttt ggaataaatg attatgtgta
     8881 tttataaaac ctgggaataa aactttacct ttagcatcta taattttttt aacatcagta
     8941 tatttacttt ctatctcttt agaatttcca atatcagcaa tcttgttatc ttttactact
     9001 aatgccccat caaaaataac ttctctctct ttattcacag ttactattat ggcattctta
     9061 ataagtatat ccatcattct tatttccccc cgcattattt agtaattttt cttttaatat
     9121 attgacctgt tggatgttct ttaacattac aactttgtac tacatgattt cctctcaaaa
     9181 atacatcttc aactttacat ttaaccttaa atccttcata tggagtataa tctacatttt
     9241 gtttttgagt tttataacta attgtttctt ctttattgat atttaaaatg actatatctg
     9301 catcacttcc tacagctatc tcaccttttt taggatacat tccaaatatt tttgcagcat
     9361 tcgtagaagt aacttccaca aacttatttg cacttattct attttctaag actccatatg
     9421 tatataaaag tgctaaccta tgttcaacac caggtgctcc atttggtatc ttactaaaat
     9481 catcaattcc caagtctttt tgccccttaa aattaaatga acagtgatct gtgccaactg
     9541 tttgaatatc tccactagac aatccttccc aaaggtaatt tatgtcttct ttttttctta
     9601 aaggaggaga cattacatac ttagcaccct caaatttatc cttattatat aaattgtctt
     9661 ctaataatag atactgtggg catgtttcta ctatcatatc tatatttttt tctctagcca
     9721 tttttacagt ttcaagtgac tccctacaac ttaagtggac tacataggat tttgaattag
     9781 ataatttagt tatatcagca agtcttgaaa cagactcttt ctcaactatg ttaggtcttg
     9841 taagtggatg atattttggc tccaaattac cattttgtat attttcttct atcaatacat
     9901 ctagtaaatc cccattttca caatgaaatc caactataca acctaaatcc cttgctttct
     9961 ttaaaaccct gtatattgtt ccatcatcta ctttcatgcc atcatatgcc atatacatct
    10021 taaaagatac tatcccatcc gaaataagtt tttccatatg attaaatgta tctttattta
    10081 aacatgtaat tgtcatatga aaaccataat cacaataaca atttccactt gcttttttat
    10141 gatatgcttc tacaccttgc agtaggtttt ccccctcatt ggcttctgca aaatctagta
    10201 ttgtagtagt tcctccagct atagctgctc ttgttcctgt ttcaaaatta tctgcagttg
    10261 taatagaacc tgcattcata tcaaaatgag tatgtgtgtc tattccacca ggaattacaa
    10321 ttttacctgt agcatcaata accttatcac catttatttc taagttattt cctatttcaa
    10381 ctatctcttc atcttctatc cttaaatcac ttatataagt cttatcagag gtaattatag
    10441 ttccacctct tagtattgtt cccatatata cctcctaact tttaagcttt aaagtatttt
    10501 ctaagtattg taaaacttta tttaaaattg tattttctaa aggtgctata ttcagcacct
    10561 ctagactata tttaaataat ttattgtgaa ctaaatatta ttttccgtat acagatttta
    10621 atattccata gtaaccttct gttcctataa ataactgttc ttgctctata tactcatcta
    10681 ttgtgtgagc aagattttcc ttagaaggac caaatcctat agtttttatt ccagcttcac
    10741 ctgcatagtg acttccattc gtacagaatg agtattgagt tatttctgaa tctattccag
    10801 cttcttttaa tcccttatat gctgcttgaa caaattcatc ttcttcgtca tataaccaac
    10861 ctgggaagaa tctttcactt tctatagtat ttcctgtata gcaatcagct ttttcaactg
    10921 cataagaaac ttttgcattt aattctgggt cttctttcat catttcatct aataaagctt
    10981 gtattggagc taatacgcct tctcttgttt ctccaactaa taatcttcta tcaaaagtag
    11041 ctttgcaata atctggcact actgatgctc ctggatatgg agaagatttt atatctgtta
    11101 aaactaatat tccatcacct aaaactggat gagttggagg tactaattgt tgtatctttt
    11161 ggattacatt agccatttta taaactgcat ttacaccttt ttctggatta gcagagtgag
    11221 ctggtttacc aaaagtttct actactattt ctcctctacc tctctgacca atctttaagt
    11281 ttaactctga agactcacct attacaacat agtcaggttg aacagcctta cttatctctc
    11341 tagccgaaac accttcaaat atttcttcat gaacaacacc tgctacatat agttctccag
    11401 caaaatctct attagtgtct ttagcaaaat acgctacagc agacatcatt gcagtatatt
    11461 gacctttcat gtcagaagtt cctctaccat atatctttcc atcaactatt tttccttcaa
    11521 aaggaggaac actccattta cttgcatctg gaactggaac tgtatctata tgtccatcaa
    11581 ataatatttt cttacctggc ttgttacctt ttatacatcc tactatattt ccatatctat
    11641 ctcttgacca actatcaaaa cctaattttt caaaagactc ttctattgct tttacaacac
    11701 cttcttcatt tccagaagaa ctaggatttt gaattaattt ttggcatatt tcagttactt
    11761 cttgttttct ttgttcgttt aacatttcat tatcccccta gattttttat aatgtatgta
    11821 ttttatatac ttatatattt tctaaatttt agaacttata tatcttctaa atatgtaaac
    11881 caatatctaa taattgcatt ttagtaattc cttatatact tggatattct ccatcccaaa
    11941 ctatacttct atatttgtca gggtcagtat ctccttctgt gcttattaaa agtattcttg
    12001 aattttcatc taattttaaa tcttttctta aatctgctaa atcttctctt tccatagctg
    12061 ctactaaaag accagttgta actgctccag attctccaga aattaccttt tcatcaccat
    12121 ttaaaggatt acctaatgtt ctcataccct tagcagaaac ccagtctggt gctgaaacaa
    12181 atgctgctgc atgatttttt aatacttcaa atcctatagt atttggctct ccacaagcaa
    12241 gccctgccat tatagttggc atatctcctc caacaaatct tggttttcca tctccagctt
    12301 ctgctgattt atagtaacaa tcagcttcat cagcctctac tactactgtt attgggcatt
    12361 catcatatat tgaagcaaca tacccttgaa ctgcaccagc aagtgaacca actcctgctt
    12421 gaacaaatac gtgtgttggt ctatctactc catattcttt taattgctct atagcttctt
    12481 gagccatagt tccatatcct tgcattatcc acgctggtat ttcttcatat ccgtcccatg
    12541 ctgtatcttg aaccattact ccatggtcat cagcttctgc ataatcatta gctaatctta
    12601 ctgcatcatc atagtttaag tctgttatac tagcatctgc accttctcct ttaattttat
    12661 ttaatctaaa ttcagaagaa ccttttggca ttaatacaac agatttttga cctaatttat
    12721 tagctgtcca agcaactcct ctaccgtgat ttccatctgt agctgtaaag aaagttattt
    12781 ctcctaattt ttcttttatt tcttttgaag ttaatttgtc ataccctaac tcagatatat
    12841 ctgtatctaa tctttgagct aagtatctac ccatagaata agaaccacct aatactttaa
    12901 atgcatttaa tccaaatcta taagattcat cttttacata tactcctgca acacctaatt
    12961 tttttgctaa accttctagg tttgctaatg gagtttcttt atattgagga aagcttttgt
    13021 gaaaatttct aaccttagtt atttcttctt catttaaaaa tttgatacaa ttttccttat
    13081 cgccctttgg caaattattt actttccatt taatttcttt taacatctaa aatacctcca
    13141 aattattgtt aaatttttat actttaatat ataagcaatt aacatgccaa cactaaatat
    13201 atactagata tttttctatg actctttagt atagaaattg caatgctttt attatttttt
    13261 cttttctatc tttagttcat acaatgtatg tagaatttaa taactaaaaa accttttcta
    13321 tcttctcact ttttaattct catttttaaa atttaaaaat aaatctcaaa acactttgtt
    13381 tctatatttt ttaatataaa atctataaaa tctaaattaa aacataaaat agtgatgcaa
    13441 taatctatta catcactatt tgtatatatt caataatata ataaatcttt agtggtctct
    13501 aagaggtgat ttttcatctt ttttaacatc taattcttct tttacgacat tccatttttc
    13561 aagaccaaac ttagaaacca taactgcctc tgctgcacct tttccaagtc tttttactgt
    13621 gtccatacct gtctcatcct cttgggcacc ttttgctttt ccatctttac tccatattgt
    13681 accacctata tagcatccac ctaaacctcc agacactaac atttcatgca ttaggaagaa
    13741 atttattata ggaagttttg tcaattcttg gccaccatat ctagttccac cagtagttat
    13801 agctgcacca accttatttt gaagttttcc tggatgaact aaatacattg gacgaagtct
    13861 attaaatact gctgtaagtt gagcagttat attcatatca taaactggag aacctactat
    13921 aattccatct gcctccaata ttttttgctc taattcttgg atatcatctt ttataataca
    13981 catagtttct tttcttatac atgcatcaca atgtacacaa ggacttattt ttttccctct
    14041 aactgaccaa tactcagttt ctataccagg tatagtttct gcttctttca aagcttctaa
    14101 cactgcatat tctgttgctc ctttacgtgg acttccacaa atacctaata tctttgccat
    14161 aataatatct cctcaccaaa taaatctatt tttattatct atttacaaat acaataaatt
    14221 gttatttctg ctttaattta tactatttta attttattgg taatgaatgt atctctacat
    14281 cagttgcatt atatactgca tttgcaacag ctggcgcaac tggtacaacg gcacattctc
    14341 caatactctt accaccaaaa ggcccagctt tttcataacc atctacaaat gcaacttgac
    14401 attttggcat atctacagtt ctaaataatt tatatgtttt aaaatcagta gctttaggtc
    14461 taccttgttc atcaaatgcc aattcttcac tcaatgcgta tcctagaccc atatgaatcc
    14521 caccttctaa ttgtccttct aaatttagag gatttataac tctaccaaca tcatgtacag
    14581 ctacaaaatc aactactttt atatccttag tttctttatc tactaaaact tcagcaaaat
    14641 gtacaccata agaagtacgt ccagctggtg aagaataatc ttcaacaaca gttaactctc
    14701 ttaatgattt actttgacta tatactgcga catcagaaag tgatgctttt acattttcat
    14761 caagtttact aattacacat ccattgtcta gatataaatc ttcttttgat gtttcaagta
    14821 atttttctgc ttcttctaat ataagatttt tcaatttctc tgctgtcttt ttagctcctc
    14881 caccttctac aaatacccct ctacttgcat agtcaccaag gttgaatgta catgcatccg
    14941 tatcagtttc aaatgtatca actttatcag gtgttattcc taaaacttca gctaccatca
    15001 ttgtttgcat agttacaaca ccattaccca tatcatgagc tcctgttagt agtgtcaacg
    15061 taccatcttc atttaattta agagtcaatg taacaacatc tctatgtgca ccaaaaactc
    15121 cattaccatg tgcacctatt gccataccta tacctcttaa atattttcct tcttcttttg
    15181 gaagtttttt cttttcatcc cacttaaaca tcttcttacc ttgctctata caatccaata
    15241 ctctaggatt tccaagagaa ccgtggaaga ttatatcgac atcatctggc tctactgcat
    15301 ttttattttg aaaatcgact aaatccatac ctatttcctt tgctattttc gcaaattgag
    15361 cttgttgagc cataaatatt tgaggtgacc catagcctct catagctcct gctattggtg
    15421 tatttgtata tacaggcatc ccagtaaact ttatatttgg tattttataa actttaaata
    15481 ctttatgact aagtgctcct ataacattta aagcacttga tgcatatgct cctgtatttg
    15541 tatatatttt tatatcttgt gctattactt caccatcttt atttacacca gtttttaatt
    15601 ttacaaaaga cccatgacga gttcttgtcg atatcataga ttcttttctt gtaagaacca
    15661 ttttaactgg tcttcttgtc atttttgata atgcggctgc tactggttca attgtaagtt
    15721 ctaattttcc accaaatgag cctccaatag caggtcttat aactcttact ttatttaatg
    15781 acatcccaaa aatttgagat agtattattc taaatccaaa tgtattttgg caaggtgtat
    15841 gtacagttaa tttatcatta taatcatagt gtgcaattac actatgagtc tctattgcac
    15901 tatgatgtat tgctggagta gagtattcat cttcaaatat atagtcacat tccttgaagc
    15961 ctttttctac atctccccca tttatgttgt tttgagaaac tatatttgaa tctccatgta
    16021 tgcagatagc atcttctttt attgcatctt gtatagtaaa tacagctggc aattcttcat
    16081 attcaacttt tataagtttt acagctttct ctgctatttc tgtagtctct gcggctactg
    16141 ctgcaacacg gtcaccaaca aatctaactg tatctgaaaa tataacttca ttttcaggta
    16201 ttttgtgctc ataaaatctt aatgaaccat tgtattttct ttgtgatgta ttgagataag
    16261 tagctatagc tctaacacct actactttct cagcttcact tgtatctata ctttttattt
    16321 ttgcatgtgc tacaggactt aaaagcatct tagcgtaaag tacattttgt ggtttcatat
    16381 cagccacgta tttcatttga ccagtaactt ttaaagctgc atcttttaga gggtatgatt
    16441 ttcctattac cttttccata attatctacc ccctaatcta gctgcagata attttacagc
    16501 ttctataatc tttacatatc ctgtgcatct acagagattt tctttaaaag cttctctaat
    16561 ttcatcttca ttaggagatg catttctatt taaaagagct tgtgttgtta taatcatacc
    16621 aggagtacaa tatccacatt gtacagctcc aacttctata aaagcttctt gtattggatg
    16681 aagattttct ccattagcaa aactttctat agtgtatata actttacctt cgcagttttt
    16741 tgcaagaagt ttacaagatt tcatgtcaac tccatctatc aaaactttac atgctccaca
    16801 actgccacta ttacaacctt gtttcgctcc agttagattt aatttttcac gaagtacatg
    16861 caaaagagtt tcattctttt caacttctat ttcatacatg taatcattaa tatttaattt
    16921 tattttttcc attttctcct cctacagata aactaatgtt gtaatagaat attattacaa
    16981 tcttatttta atattagtat aagctattcc acttatgtcc ttccattcta ttagttacat
    17041 tagctctctt acataattct tctgctgcat gttgtgcatt aagcaacact tgttcttcac
    17101 tttcaattgt agttactttt ccattttcaa gtattacatt tccatcaact aaaacactct
    17161 ctacattgtg catactagct gagtatacca aagttgatac aggattgtga agaggtattg
    17221 attttggaga aagcattgag ttaaatatta ctaggtcagc ttttttccca atctccaaag
    17281 aacctatttc atcttccatt ctaagagttc ttgctccctc aattgtagcc atttcaagaa
    17341 ctttctctgc agaaattgcc agagggtcac aattatttac tttgtgttgt aaagctgtta
    17401 gtttcataag ctctaccata tcttgtgcgt tattacttgc tgcaccgtca agacctaaac
    17461 tcacatttat accttttttt aacatttctg gcacaggtgc aacaccagaa gcaagataca
    17521 tgttacttgc tacattatga gaaactttta tgtcatactt tttaagcatt tccatgtctt
    17581 catcagttat acatacacaa tgtactgcta caacttctgg acctactata ccccattttt
    17641 caagtaattt tatatctatt tctccatgta tatcctttgt tgctgttctt gcaaattctg
    17701 tttcagatat atgaactgta aaatatgaat catattcttt aactatattc caaagcatct
    17761 tacccatttc ttcagatata gcccacattg aagaaggagc tacacagatt tttattcttc
    17821 cattttctga attatgatat ttttcgaata cccttctcaa atctttttca accgtttcca
    17881 catcttctat aagctctttg tgaataccta aatctataca acctctacct acaacccctc
    17941 ttatacctaa atctttatat gcctttacta ttccatctgt aagtcctggt ctattgtgag
    18001 tatgcatata atcaaccatt gtagttatac cactttttaa accttccata catccaagca
    18061 ttgcagcatc ataagtatct tgttctgtta agaattttgc tgatggaaac atcattgtat
    18121 ttaaccactc atgaagtgcc atatcatcac ctaaaccttt taataacact tggaacaggt
    18181 gattatgagt atttataaag ccagggaata ttacttttcc attagcatct ataacttttt
    18241 tagcatcaag atacttaggc tcaatatcaa ctgtatttcc tatgtccaag attctgtcac
    18301 cttgaatagc tattgctcca tcaaatataa catctctgtc tttattaact gtaacaatta
    18361 ttgcattttt tattagtata tcaatcatct taaatccccc tttgaatact ttcattacaa
    18421 cattttacca tatggttttc tcatattata tctaatacat ttatccaata ttttcctcaa
    18481 gatttgaagt aacgttttca gcacttttac gtgactttaa tttaaataat gtttgaagta
    18541 atatagctat tattgctcca gaaactatac ttgaccctcc aaggacagtt ccaattgtct
    18601 ctgggaattg gtctaaaact tgtggagcag ttgtaagacc catacttgtt gctatagata
    18661 cacctgctat aagattgttt tcttcagtga atccatccat actcataata gagaaaccag
    18721 acattgctat tgatgagaaa actactaaag ttgctccacc aacaacacaa gatggtatag
    18781 ttgtcattat tgcacccaat gctggagata tccctgctag taataataca agtgccccta
    18841 gtgctattac aaatctactt gtaactttgt tcatagatac aattactgta ttttggctaa
    18901 atactccagt tggaagagct gcaaatagtg atgaaattat agaagttata gaatttccta
    18961 aaacaactga tgctaattct tcgtcagtta cctctctatc cattgctcct actgtagcta
    19021 ttgtacaggc acccatcata tcagctatac ctattatgta aataatagta aacattacta
    19081 ttaactctgg tctaaattct aatccaaaag ctgctggtgt tggtaaagat actatagctg
    19141 ctccttgaac ggctgagaaa tcaaccaatc ctaaaactaa agatatagca taacctacta
    19201 atattcctat taatattgaa gaatttttta ctaaaccttt accatattta tttatcatta
    19261 ttattacaaa agcaactata actcctactg tgaagtttat tgggcttcca aatgtttgtg
    19321 tgccttctcc tccagctaaa ttctttatag ctgttgcgaa tagtccaagt cccatacatg
    19381 ctattatagt tccagaaact attggagtga atatatgtct aattttctta actccaaatc
    19441 ctacaaagaa tacgattacc ccagcagcaa tctgtgctcc aaatagagct gctaaaccat
    19501 ctttagctgc tactgaaaga caagctccta aaaatacata actcattccc atcattacag
    19561 gtaatcttga acctaactta aatcctttaa ataaaggtat tggatataac tgtaaaaacg
    19621 ttgctaaacc tgctgctaac atagaaccct gaatcaacat tgttgcttgc ccctggttaa
    19681 gtcctaccac gtttgctatt aatatcgcag gtaccatatt tcctactatc attgctaaca
    19741 gatgttgtaa ggccaaaggt attgattcct ttagtggtgg tatcccatcg acatcatatt
    19801 ttgaagtatt acgtgttttc ataaaataaa tcctcccata cataaatatc ttgactaaat
    19861 atttttataa ttattttcta taaatatttt tatgaccatg aatttattgc tcaaccgata
    19921 aacacagcta aactgagcta tttttataaa aaattaacaa atttctaaat attccctccc
    19981 cgattttcaa cttaaatgaa cattaattac ctggtatata tttatttgtt aaatttttta
    20041 ttaaaatgaa tagctatcaa aaataatcta acaatctcat gctaaataat ttaaacaatt
    20101 cttttaaatt attatatctt aattagtctc ttccataaca gctctttctt tagcctcatc
    20161 ctctagactt ttatttggta ttattagatt taaaacgaat gcaaccaatg ttgcaattac
    20221 tattggagat tctccaaata ctaactttac aaatgaagga aatgcctctt gggctgctgg
    20281 tactgatgtt attcccatgc ctaaggcaat tgccaaacca acaatggtaa tatttcttga
    20341 tgacaactct tctgtaataa ttaactttat acctgtcatt gttatcatac taaatataga
    20401 tattgtagct cctcctaaaa cagaatatgg tatagtagtc attaatgcac caaatttagg
    20461 cacaaacccc gacaataaca taaatatacc agctattcca agcacaaatc tacttattac
    20521 tttgttcata gctaccatcc ccacgttttg gctatatgat gatggtggta aacctccaaa
    20581 caaagctcct atcatagtac aaattccact tcctattact ccacttgata attccttatc
    20641 tgtgatattc ctatccatgc ctcccattgt agtagctgat aaatctccaa ttgcttgaac
    20701 agcattgatt atacttacaa ttactactga gattattaca ggcatatcaa attccatccc
    20761 aaattcaagc ggtttaggta tcgcaaacca agttgcctct ctaatagggt caaattttat
    20821 tatacccaga actaaagata cacaatatcc aactataatt cccaaaaata ttgctgccaa
    20881 ttttgcatat ccttttgcaa attgactaag acctataact accactaatg ttatagccgc
    20941 tactgcccag ttttgcatag aggcataagt aggactattt acaccacctg ccatataatt
    21001 tattgctata ggatacaaag ataatccaat tgtaaaaacc actgttcctg caactatatt
    21061 cggaaaaaat tttcttatct tttttatgaa tgtccctatt aatactgtag ccactccccc
    21121 tattaattgt gcacccaaaa tgcttgctaa tccataattt cctccgactg ctacaagtgt
    21181 tggtacataa gtaaaaccaa ctccaaaaat tataggcaat cttgaaccaa tatttcctat
    21241 tggatatagt tgtataagtg ttgatatacc agctattatt aatgcatatt gcactaaaag
    21301 tgttatttgt gtagggctaa cacccacagc tcctcctacg actataggta ctgtaacagt
    21361 tccaactatc attgctagta tatgttgcat tgatagttgc aaacacttta aaattggtgg
    21421 ccttccatca atatcaaaca tagaaacatt cttttgatat ttactcaagg ttatcactcc
    21481 caactatttt taatgacagt tattattatc tcacacaaca tcctttcttt ttttattttg
    21541 tcatctcaac ttaatatgaa gcaattataa tgccaatttt ttaacataat tgttttacat
    21601 atataatttt tattatattt gttagtattt gataatttat taaagcattt atttttttaa
    21661 attcttttaa atttttttta tttttggata ataaaatgct attacacaaa aaaacactta
    21721 gaaaatagtt ttccaagtgt tttttatcaa ttttataatt tcaaatttta gaattgagaa
    21781 taaaaagcct aattattata catctatttt tctgtacaat gtagctagtg atattccaag
    21841 ttttttggca gccagttttt tagacttagt atcatttccg taaaacttta acactttctt
    21901 tatataggct ctttcaaact cttcaagtgt aagtatgtct tgttccatac ttatctcata
    21961 gtcactttcc tcaagtaact ttatatttat attatttaac ttaaaatatt caagtatctt
    22021 tctatttatt attcctttat gtattattcc atcttcctca agaaggttta atgccagttc
    22081 tatagtattt tccaactctc taatatttcc tggccaagga tacttaagca acatttgttc
    22141 cacatctggc tctatatggt acacgttttt cttcatcttt ctcgaatatt tgtctataaa
    22201 attgtttatt aatatttgta tatcgtctcc tctttctcta agtggaggca cttctaaagg
    22261 tattacattc aatctatagt ataagtcttc tctaaattta ttttccttaa ccaactcaaa
    22321 taaatctcta ttagtagctg atatgattct aacatctata tcaattagtt catttgaacc
    22381 tattctcgta aacttctttt cctgtaatac cctcaataac tttgattgta gttgaattgg
    22441 catatctcct atctcatcta aaaatacaac ccctccattg gccaattcaa acttaccaat
    22501 tttacctttt gaatctgctc cactaaaagc tccttttgta tatccaaaaa gttcactttc
    22561 tagtaaaggt tctggtatag cagcacagtt tatagcaacg aatgttttat tccctctgtt
    22621 tcctgcgaga tgaatggctc ttgctattaa ttcttttcca gtgccacttt ctccagttat
    22681 agctacagta gaatcactat ttccaatttt acaaattcta ttcttaagag cacgcattac
    22741 atcagactct ccaattatgg agtctactgt tgttgttccc caaatattat atacttccat
    22801 tccaccatac attactgaat cactgtgttt gtccataaaa tatcaactcc aaataagtat
    22861 tctatataat aatcattcac attatctatt taattatatc ataacagaaa agcttagtta
    22921 taatttaata atattatttt aaaacaaaac ttaataaaaa gtatatctac ttagttttaa
    22981 tttgatagat atactttctt cattttacaa aaatgctcta ttaaataagc aattacaatg
    23041 ccatatttta tatggaaata atttttaa
//
LOCUS       DAEMIK010000054        23043 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEMIK010000054 DAEMIK010000000
VERSION     DAEMIK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.054"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            316..1152
                     /locus_tag="KPK87_001790"
     CDS             316..1152
                     /locus_tag="KPK87_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2537942.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1505..3082
                     /locus_tag="KPK87_001791"
     CDS             1505..3082
                     /locus_tag="KPK87_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2537943.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPVEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3075..4259
                     /locus_tag="KPK87_001792"
     CDS             3075..4259
                     /locus_tag="KPK87_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG2537944.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLKHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEESEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4277..4876
                     /locus_tag="KPK87_001793"
     CDS             4277..4876
                     /locus_tag="KPK87_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2537945.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFIHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4900..5370
                     /locus_tag="KPK87_001794"
     CDS             4900..5370
                     /locus_tag="KPK87_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2537946.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIIVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5729..6475
                     /locus_tag="KPK87_001795"
     CDS             5729..6475
                     /locus_tag="KPK87_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG2537947.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHVELEHSDYLEQLINSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6536..7210
                     /locus_tag="KPK87_001796"
     CDS             6536..7210
                     /locus_tag="KPK87_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG2537948.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTHGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7579..8925
                     /locus_tag="KPK87_001797"
     CDS             7579..8925
                     /locus_tag="KPK87_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2537949.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLGGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMYYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8952..10352
                     /locus_tag="KPK87_001798"
     CDS             8952..10352
                     /locus_tag="KPK87_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2537950.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10562..11998
                     /locus_tag="KPK87_001799"
     CDS             10562..11998
                     /locus_tag="KPK87_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2537951.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIVSNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            12342..13286
                     /locus_tag="KPK87_001800"
     CDS             12342..13286
                     /locus_tag="KPK87_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG2537952.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEIFKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEGNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13455..14387
                     /locus_tag="KPK87_001801"
     CDS             13455..14387
                     /locus_tag="KPK87_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG2537953.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14492..14959
                     /locus_tag="KPK87_001802"
     CDS             14492..14959
                     /locus_tag="KPK87_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG2537954.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15203..16057)
                     /locus_tag="KPK87_001803"
     CDS             complement(15203..16057)
                     /locus_tag="KPK87_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG2537955.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16223..18187
                     /locus_tag="KPK87_001804"
     CDS             16223..18187
                     /locus_tag="KPK87_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2537956.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18187..18648
                     /locus_tag="KPK87_001805"
     CDS             18187..18648
                     /locus_tag="KPK87_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2537957.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18651..18971
                     /locus_tag="KPK87_001806"
     CDS             18651..18971
                     /locus_tag="KPK87_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2537958.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18987..20087
                     /locus_tag="KPK87_001807"
     CDS             18987..20087
                     /locus_tag="KPK87_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2537959.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20207..22807
                     /locus_tag="KPK87_001808"
     CDS             20207..22807
                     /locus_tag="KPK87_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG2537960.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGIKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPAIGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         9064 a         2355 c         4060 g         7564 t
ORIGIN      
        1 tataatctga atttcaataa atatatatat aattaagttg aaatacttga aaatactgga
       61 aaaaattgaa aataattttt gtaatataaa atgtcgattg attttaaata attaaatata
      121 cataagaaat aattaaatat atttcaatga aaatatgaca aattaattga tgacaataaa
      181 aaatattttg ttattgtagt attatagggt aaattgcaaa atatatacga aagtaaaatt
      241 ttaaatattg acattaaatt aaatatacta tactatgtaa gtgtgatgag agaatttttt
      301 tagaggggat ttatagtgaa tataaaaaag atatttaata acaatgtagt agtaagctcc
      361 ctagaagatg gcacagaaat aattgtcact ggtgctggag taggatttaa gaaaaaagta
      421 ggcgatttga tagatgaaaa tttgatttct aagaaatatt ttgttcaaga cgatcaaagg
      481 gacaaataca atcaaatact taataagacc tctattgaat actttaaaat ttcagaagaa
      541 attatagaaa aggctaatga agttttaaat actcaagtga atgattcaat aatattagcc
      601 ttaactagtc acatagaatt tgctgtccaa agagaaaaac aaggtataaa attaccaaac
      661 ttaatattga atgagacaaa acaactttat agggaagaat ttgaatttgg attatgggca
      721 attgatgaaa ttgaaaaaag gataggaata aaattgcctg aagatgaagc aggatatatt
      781 gctattcaca taatcaatgg tttggaaaat tctcaaaaag atgaaggtat taatatacta
      841 gagttttcaa aacaagttat tcagataatt gaagaagtac atggatttaa attggatgta
      901 aattcattaa attatactag acttataact cacttaaagt tttttgttca aaggattctc
      961 cgaaaagaaa cttacaatga tagtgatatt gaagatatgt ataagttagt gaataaaaat
     1021 tacaataaac ctaaagtgtg tactgaagaa attgcagcat tggtattaga cagatttgaa
     1081 tataagatta gtaaagaaga agaattatat ttaatgattc acataaataa aataacaaac
     1141 agtggttgtt aggattgtaa ctgataaagc aggcaaaacc taagtcattg agcttaacta
     1201 ttagattaag ttttttgatt taggtttttt tattagatac aatcaaaata cagacagtga
     1261 cttttaggat tgtaactggt taagcaggca aaacctaaat ctttgaattt aactattagg
     1321 ttaagttttt tgatttaggt ttttttatta gatacaatca aatacagaca gtgactttta
     1381 ggattgtaac tggttgagta ggcaaaacct aaatcattaa acttaattat taaattaagt
     1441 tttttgattt aggtttttct ataaatttga ttatgttttt agataaaaat aggaggggaa
     1501 aattatgaaa aaaaataagt taggtttatg gaacttcttc caaatgttag gaaaaacgtt
     1561 tatgttccca atagcactac ttagtgtttc tgggatgatg ctaggacttg gggcaggttt
     1621 tacagaccct aagatgattg aaatggttcc atttttaggg aatgaatatg ttaacttagt
     1681 tttaaacttc atgttaacaa taggattatt tgcatttaac aacttgggag cattatttgc
     1741 aatggcaatt ccactaggat tattaaaaaa agaaaaagag tttggtgctt tttcaggatt
     1801 agtaggattt atagcaatgc acataggtac aaatttttat ttaacaagag ctgatttgtt
     1861 agtaccagta gaacagatgt caactaatgg tcaagctatg attatgggta tacaaacata
     1921 taatacaagt gttttaggtg gtattgtagc aggattaatt gtttatgcaa tatatgataa
     1981 agttagtaat ttaaagatac cagaatcatt aggattctac agtggaccaa gattagttcc
     2041 aattgttagt ttaatagtta tgagtatagt tggattatta atacctattg tttggccacc
     2101 atttttcaat gcattccaaa gtttaggaag atggatttct tcagcaggac cattaggata
     2161 tttttcatat gcagtggcag aaagagttac aattccattt ggactaaatc atcttgtaac
     2221 atccgtattc cgttttacac caataggtgg aactgctgtt attgatggag aaacgtactt
     2281 tggtacattg aacatgttta tggcatatgt agaaaataat cagattatac ctcttgattt
     2341 agctggtaaa atggaacaag gtaaattgat gattcaatat ggattagctg gtgctgcatt
     2401 ggctatctac cgtacagcaa aaccagaaaa tagaaaggca cttaaaggac ttttaatatc
     2461 aggagtgttg acagttatta taggtggtat aagtgaacca atagagtttc tattcttatt
     2521 catatcacca gcattatttg ctttccatac atttatgaat ggtctagcaa acatggtact
     2581 tccatactta ggcgtgttga tgggattcac aggagattta attgcattta taagttttgg
     2641 tgtgttaaga ggaacagcta caggatggcc aatagcagta ttagtagcag ttttatattt
     2701 tgcaatatac tattttgtat ttaagtgggc aattattaag tttaatataa agaccccagg
     2761 acgtgaggat aaagaaataa aagtatctga aggaaatggt tcagatttca aaaatttgtc
     2821 tacatataaa ggacaacaaa tgatatctgc tttaggtggt caatctaata tattatcact
     2881 agataattgt gtaactagat tacgtttaaa attacaagat gtatcattaa taaatgagga
     2941 tgcaattaaa gaggctggtg gtatagcagt tgttaaattg gatgagcata caatacaagt
     3001 aattatagga actcaagtgt attctttaag aaagcagatg gataaagcta tggaaggtca
     3061 tttggaggca tgtaatgatt aattttgata aaaaagtaga ccgattgggt acatattgta
     3121 cccagtggga ctatgtagaa gatagatttg gtaagaagga tttgcttcct tttacaattt
     3181 cagatatgga tttagagttt ccatttgaaa taacagaggt gttgcaggaa aggttaaagc
     3241 atagagtttt gggatacagt agatggaaac atgatgattt tagaaattca gttacgaatt
     3301 ggtatattaa aagatttaat tgtagcgttg atagtaactg gatatattat agtccaagtg
     3361 taatgtattc aattagcaaa cttattgaga tgttaagtaa tgaaggagat ggaatcctta
     3421 taaatactcc tgcatacaat gctttttatg atgtgataat aaataacaat cgtaatatta
     3481 taaagtcaca tcttgtaaat aggaatggat tctattcaat tgattttaat gattttgaga
     3541 aaaagtgtaa aaaatctaag atatttattt tatgtaatcc tcacaatcca actggtagag
     3601 tatggagtga gttagaactt gttaagatga tagggatttg taagaataat gatgtcaaga
     3661 taatatcaga tgatatacac atggatattg tatatgacaa tagaatgaca cctatcttaa
     3721 atgttgcaaa ggactattta gattctgtat tcatttgtac atcagcgtct aaatctttca
     3781 atataccagc attgactggg tcatatgtga ttataccaaa tgaaggtatt agaaataaat
     3841 ttgaaaatat cactagatac cgtgattttg taaattctcc agcaattcta gcaatattag
     3901 ctacaatggt ctgttatgat gactgcgaat attggttaga tgaactatta aaatacctta
     3961 aagagaatct taaatatacg attgattata taaaagataa tttagaacca ttgaaattgg
     4021 tcatgccaga aggctgttac tttgcttgga tagatttttc cgaacttaaa ataagtggtg
     4081 agaaattcca aaaattattg atggatattg gagaagttgc tattatgtcg gggtctgtat
     4141 atggtgaaga aagtgagttt tatttaaggt taaatgtggc atgttcaaga gaaaaacttg
     4201 aagatggttt aaataggatt aaaaaaacta tggattatat tgcaaaaaga attttatagt
     4261 aggaagggag atagttatgg gatatttaaa aacaatggtt gatgttatga ctactgaaat
     4321 gaatgccata agatgtttaa tagatgaagc aggaatagaa tatgaaagta tagttagtga
     4381 aattgcaaat tgtgaaggca aagtaatttt tatgggtgta ggaaaatctg cacatatagg
     4441 taagaaattg gcagctacat ttgctagtac tggtacacct agctttttta tacatgcaac
     4501 agaggcagtg catggagatt taggaatgat tgaaagtaag gatattacaa ttttaatatc
     4561 aaatagtgga aattctatgg aagtggtaaa ctgtataaaa tatattaaag caataggttc
     4621 taagacaatt gcatttactt caaacagaaa ttcagtactg gcaaaagagt gtgattatgc
     4681 tttaatatat cctgcaaagg atgaagcaga ccatttaaat ctagcaccaa caacatcatc
     4741 tacaattact ttggttttag gagattctat tgcatgtgca ttatctaaga gtagtaattt
     4801 tggttcttca gatttttata agtaccatcc aggaggaagt ttaggtgaga agttaaaaac
     4861 agctaacaat ggataaaagc aaaaaggaga gtagttttta tgttcaaaat gtttaaaaaa
     4921 aaatcaataa aatcacccat atcaggaaaa gtaatagagt tatctaaaat acaagatggt
     4981 gtattttctc aaaaaattat gggagagggt gtagctatag attctacagg agacattgta
     5041 tatgctccaa tcaatggaaa gataatagta gtagcagaga ctaaacatgc atttgtaata
     5101 gaattagaaa atgggatgga acttcttatt catgttggtt tagatactgt aaatttaaaa
     5161 ggcgaaggtt ttgaggtatt ggtgagtatg ggagaacaag tgaaggaagg aacaccaatg
     5221 ttgaaaatta accgtccttt gattgaatca aagggtatat cacttattac tccagttaca
     5281 ataactaatc attcagagta caatatgaat acatgtaatg taggcaatag tgttgaaggt
     5341 gggaaagata ctgtaattga gtttaaataa agaactaaaa tacaaggtta atatttataa
     5401 atacctataa acttatgttt tataagattg attgtataga tttgttgtat aaaaaagaac
     5461 tctttaatta taaattaagg agttcttttt tattttgtaa atattgttta gatttatata
     5521 gaaaaacact tagtaataaa tagaataaat aagtatttat atagctaaaa atctaaatag
     5581 ttttgtttaa atatattttt atattttgta atttcaaatt aataaataat tagatttgat
     5641 aaacaaaaaa taaaaatgtt gattacatta ataaacttta atataatata tttatgaaaa
     5701 atgtaaactg tgaaatggag gtagtaacat gaaaattgaa aatctttcta ttgatgatat
     5761 taaacgaggc tatatattta atgatgattt aaattgttat agttgcatta aatgtacaaa
     5821 aacatatgag gtaggagaaa tatataaaat aggtaataga ttctttgaag cttcaaaagc
     5881 tattgaactt catgtggaat tagaacatag tgattattta gagcaattga ttaatagtga
     5941 ttcaaaatac aacacattga ctgaaaatca aaaacaatta tttcaattat ttattagtga
     6001 tttaccagat aaggagatag caaaacaggt tggagtatcc acatctacta tacgccatca
     6061 aaaatttatt ttcagagaaa aagcaaaaca ggctaagttc tatttagcac tatatgaatc
     6121 tgtatttgaa gataaatcta ataaaagtag tactataata ccaatccatg aacatgctcg
     6181 aatggtagat gaaagatatg tagtaactga agaagagaga aaacacattt tggatacatc
     6241 atttgaaagt atgaatcctt taaaattaaa agtattttct tcaaaagaaa agaaaaaggt
     6301 cgtaatttta tctaagattt tggaacaatt tgaacgtggg aagaaatatt cagaaaagga
     6361 agttaatcag attttagaat ctatatatga tgattttgca acaattagaa gatatctaat
     6421 tgagtatggt tttatgagta gaaataaaga atgtacaaag tattggttaa cataattaaa
     6481 ataataaatt ttactaatgt gtaatgttat ttttatctag ggggaggagt caaatatgaa
     6541 tgatgaatta aaatctattt tatacaataa aggtgtggac attgttcgct ttgttgatat
     6601 atctgaattt ccaataaatc aaacgcacgg tttttctaaa gcaattttat tttgtatagg
     6661 gttatcaaaa aaatttatta cagatatata caataactta cctacagata gtgatgagtt
     6721 tttggaaaaa gaggagaaag tagaagaatt agctgactgg atttcaaaat atattcaaaa
     6781 taaaggatat agagcgtatt ctcaatcgga gaaaaacaat ctagaacatg gatattttga
     6841 aaaagcctat attaatccag aaatgcaatc agggattagt cctttaccac ataagacaat
     6901 agcaaatatt agtggcattg gatttatggg aaaaaataat ttgtttgtaa cagaggaata
     6961 tggatgtgct tttagcatgt gtacagtact tacagatgct ccaatttctg ttgaaagata
     7021 tcctttgatt gattcaaaat gtatggattg taatgtttgt gtagaaaact gccctgcaaa
     7081 agctatacat ggaaatgagt ggactttgcc aggaaaaaga gagtctatta ttgatgtttc
     7141 aaagtgtttt tgtgtattga ggtgtatgat gagttgccca tggtcattaa gatatgcaaa
     7201 tcaaaaataa ctttactaga gaaggtatta aattgaatat tataaaattt ttaaaatgta
     7261 agattatata agatttctca aaataaaaat ttgtatagaa cactcagaaa tgagtgtttt
     7321 tttattggaa ttaaattata ttgatacaaa gcttaactat tttaatcatt atataaaatt
     7381 tgtattaaaa aattaagaat aacttggtaa tagtgaaaat ataatgagat tataattgat
     7441 ttataatata actataatga tattgtaaaa atgaaaattg tcctctcaaa attcccaaag
     7501 agagtaaaag tcacttcagt tatagtcaaa aaaatttttc tgtgaaaaga atttagattt
     7561 gaaaaggagg aattaatcat ggaaattgaa actaaaactc cagattggat taatgtacta
     7621 gaattaaaca gtaaaataga tattactggg aaatatgatg taagtaatat gatacaaaat
     7681 attgtctctg ataagtcact aggaggttcc attatatatt ttccaaaagg taattactta
     7741 tttgagaaag gtattaaaat tagtcaacaa ataacacttc aaggagattc ttattatgga
     7801 ggaggtaatc aagtatcaaa tttagataaa aaacaatcaa tcataggaac aaccaatttt
     7861 ataacccgag gagtttctaa tatgagtatt attacattga caggaacaag tcaatgtatc
     7921 aaaaatatta atttttatta tgatagtcat gatatagaga aaataccgcc taaaaatgta
     7981 tctgctatta cagaatatgg agaaacacaa ggattatctc attttgaaca tctgtttatt
     8041 tctggatttt cagggatagg aattgaaatt ccatactatt caactggtaa tgatattact
     8101 gttagttcat gtggtcttgg tatgaggctt ggagagaagt caatgctttc tagtagcaag
     8161 atttacgaat gtaaaaatgg aatggagatt actactggtg ttagcttaaa taatgtgaga
     8221 atagaagaaa taaaagaaat aggtattaat aataaaggat ttggttttaa tctgattatg
     8281 aatcttatgg tcgaccaatg tggatattgt ggatttatgt ttgaaaaaat gtactatagt
     8341 cagataactg ccagaattac tagatgcggt cagtattata aaagtgttga ttataatgct
     8401 tatgagaata tggaagatag aaaagaagaa gcttattcta ttttttatgg agatatttta
     8461 gaaaattgca atattgtatt gatgaatagc aatattgata ccttagaaag tgattcactt
     8521 aatgaacata agatatatgt aatcaaggca aatgaaacaa acaatgtaat gctaacatgt
     8581 tatgctgaag cagataactt tatacaatgt gcaaagggca acttgctgtt agaaaatggt
     8641 gaaaatactt ataaatttta taatggtgag atttgttctg taggaggtgt tcaaatttct
     8701 gatatagatg aaaaagattt aatcaaaata atagagaaca ctttgtatat agatgataaa
     8761 ggtaagtatt taatggttcc tagattaaaa aagaatactg taatatattc agtactagat
     8821 tctacagaag aaataaataa attacatggt ggaatatggg aacagattga tgtaaaagta
     8881 ttagctgggg aatccatgta ctattataaa aagataaaag attaaattat caaactagga
     8941 ggtagtgatt aatggaagag tctataataa ttaaagatat aagtggctgg attaatgtac
     9001 ttgattatgg aatttgtaat gatgaaacaa aagatgtaag ttttaagttg caagagatta
     9061 tggatgctgt accagaagat tctattatat attttccaaa aggaaaatac ttatttgaaa
     9121 gtggtgttaa gattaagaaa cgattaacct tatgtggaga ttcttatatt ttagcgaatg
     9181 ttcctaaaaa tactgctgga gtaacacagt ttacttttaa aggaaagaaa aatcctaaaa
     9241 gtgaaaatat agcaatcata acagcaaaaa gtgtaagaca ctgtattaag aacatttgct
     9301 tttattcaga tagttgtgat agagatatga catctaatca tgaacctcca acagatggaa
     9361 aacctagatt tcatcataat gtaattataa attatagaaa tatatctgct gttatatgtg
     9421 aagataaaca gggagaacca ggtcattatg aaaatctatc ttttagtggt ttttctggaa
     9481 ttgcacttga tatgcctaat aattctacag caaatgatat tacaattttt acatccggac
     9541 ttggaattag tacaggggaa aattcaacaa ttgctaacag taaggtttgg ggctgtgttg
     9601 atggaatgat aatttctact ggaactttta taaatggtat gagggtagaa gaaataaaaa
     9661 aagttggaat taaaaatatt ggtagaggtt taaattttat tacaaatatc acaattgacc
     9721 aatgtggata ttgtggagtt tggtttgatt ctatatccaa tgttcagata tcaggaaata
     9781 tcacaaggtg tgggcagtac tattatgatg taagctatga taattattta atgttggata
     9841 atagagttaa agaagcatat tctttattct atggtaattt attggagtca agtaatatag
     9901 ttttatcaaa taccaatgaa gataattggg aagatttcaa agaagataag cataaggtgt
     9961 atcttataga agcacttaaa accaagaatg tactattaac atgcaatata gatgctactg
    10021 actggattgt taagtcagaa aaaggaaatt taacgtacaa taatatgcga caaactgtta
    10081 tattttacga tggaaaacta agtagtatca atggaattgg tatatctgat gaagattatg
    10141 gggataaagt caagataaaa aaaggaaata tatatataaa tagtaatgat aaggatatta
    10201 agatgcataa accagaggta ggtgacatta tgtcaacaat tattaaagat aaaagtattc
    10261 tttctgctca ttatggggga acatgggaga aaattggtga aaaaatagag tttggaataa
    10321 ctgtacacta ttataaattg atagagaaat aagtaaatac gtagtatttg aatagatgta
    10381 ttttattgga gagctaggac tatagtttta atataaatgg tttggtaata gcatggattg
    10441 aataatataa aaataatata aaaaattaat ttgattatgt aaatttctat gaagtagtaa
    10501 aactgtgact ttagtcatag gaggttcaaa ctaagtaaaa gggaaaggaa tatataatat
    10561 tatggaagga agaactggga ttaaagatgg aagatggatt aatgtagtag aaagaggtat
    10621 taaaaatgat ggttcgtaca acataggtcc tgctttgcag gatattattg atgaaataga
    10681 aacagatttt tgtgtcttat attttccaaa aggtaaatac ctatttacaa gtggtgttaa
    10741 aattaataaa gaaattgtat tgcaaggtga ttctaattca tctacaggta ctacacaatt
    10801 tataactcgt ggagtagaaa atatgagtat tattacattg acagggaaga aacaatgtat
    10861 taaaaatata aatttctatt ctgatagttg tgagatacag gtaaataaag aaccaccgac
    10921 aaaaggtaat cctaaatatc attatgagat gattattaat aagaatgaaa agaatgttga
    10981 gatgaataat gtatcagcaa tcctgtatga taacagtaag aatatgatta aaggtctagg
    11041 tcattatgaa aatatttata taagtggttt ttcgggtaca ggaattagga taccatatta
    11101 ctcaattgta aatgatatta cagtttcatc ttgtgggctg ggtattgata caggaataga
    11161 cacaattgtt tctaatagta gaatatctaa gtgtcaaaat ggaatgagga ttaggactgg
    11221 aactagtata aataatgtaa ggattgaaaa aatacagaaa gttggtctta taagtagtca
    11281 agttaagggg tatgaggggt atggaagtta taaaattaat aatattacga ttgaccaatg
    11341 tgggtattgt ggcttttcat ttgattcaat gaaagatagt tatttttctg gtattataag
    11401 aaggtgtggt cagtattatt ataatacaga ttatgacaca tatttaagta ttaaagatag
    11461 agtagaagaa gcatattctt tattttatgg aaattatttt acagaatgta atattgattt
    11521 agttagtgat aataaggata actgggatga tggcttagaa tataaacata aagtttatgt
    11581 atttaaaacc cttcaaatac caaaattaac attgagatgc aatatagatg cctatgatta
    11641 tatcgttaaa tcggtacatg gaggaggtaa tttaatgtta aaaaataact ttaacaatta
    11701 tatatttatt gattcatcta atccagaagt tattaatgga attaatttta attatcaaaa
    11761 gaatggaaac ttgttaaggt taaataatgg taaattaagt tataaaaatg gaaaggaaga
    11821 aacagctata ttcagaccta attatggtct tattgtagca tcaacagatt cagaatgtga
    11881 acaaatagag actagctatg gaggaaaatg ggagaagctt ggttctgaag taattggaga
    11941 agaaacggta tattattata aacatcatga accagataaa acaaaccaaa tacaataaaa
    12001 tttgtattat ataatgatat aaagttttaa tatatagtgt ttacctttaa tgtaatattg
    12061 gtatatttgt atagtattaa atttgttaaa aagtaaaatt gtagggtgct tgaaaatagc
    12121 acctttttta gtttcattat aaaattagtt ttgaaagttt aggaataatg tatatttatt
    12181 tttaaagaat tataacctta ttaaggatat tttaaatgct agaaatgaat cagtaaatca
    12241 tactggaatt atcttaaatg ttttgattaa attaagtatt tagagctata attatattaa
    12301 aaatatatta gaacataatt atattactat ggaggttaaa tatgatagta agtgtgagta
    12361 gaagaactga tatacctgct ttctatagta agtggttttt taacagactt gaagaaggtt
    12421 ttgtttatgt aattaatcca atgaatccaa aacaggttag taagatagag ttaaatccac
    12481 atactgttga ttgttttgta ttttggacaa aggatgtaac tccaatgtta tatgatttaa
    12541 ataaattaag agactataaa tattattttc actatactat aacatcatat ggaaaagaag
    12601 tagagacatg tattttagat aaaagaaagg taatagattc atttaaggaa ttgtctagaa
    12661 aaataggcaa agaaagagta attttaagat acgacccgat tttcttaagt gaaaagtaca
    12721 cagtagatta tcatatcaaa gcatttgcga gtttatgtaa tcagcttgat ggatttacag
    12781 aaaaatgtat aataagtttt atcgatttat acaagaaaac aaaatttaat actaaagcat
    12841 tacatataaa gccaatagaa attacagaaa tagaaacatt atcaaaagag atttttaaga
    12901 ttgctcataa gcatgggata acacttgaaa tgtgttctga agaatataat ttaagtaaat
    12961 ttgggattgg aaaaagtaag tgtattgatg atagattaat atcaaaaatt ataggatgtg
    13021 aagtagaggt aaaaaaagat agtacacaaa gagatatttg tggatgtgtt aaaagtgtcg
    13081 atataggaca atacaatact tgcagacatt attgtctgta ttgttatgct aactttaatt
    13141 atgcaaaagt agaagaaaac tgtaggcttt ataaagaagg taataaatta ctcatagata
    13201 ctttaagaga tgatgcaaaa ataagcatta gagatatgaa gactattaaa gtagataaga
    13261 ataaacaaat tagtatattt gattaaaata aatatactaa ttaccttaat atattaagaa
    13321 tatagatact tttgataaca aaattaaact taatagatat tttctatata agaaagatgg
    13381 tcataatatg tcaatttgac catatttttg atataattaa aacacaaata atttagtaac
    13441 aaaggaggca gaacttgaat ttattaaaaa aatatcctat acctattgca agtttgattt
    13501 tgtcaatctt tactttaggt aacttattac aatcttatag tgaatcatta cgtaatgtaa
    13561 ttggatttat aggatttatt ttgtatgcaa tttatatagt caaaataata ttacttagaa
    13621 agggtgttaa agagcaatta gagaaccctt taatatcaag tacatttcca actataacaa
    13681 tggcaacaat gatatttgct acatatataa aaccattatc tttagaatta agtattatga
    13741 tttggagcat aggaattgtg ggtcatcttg tgttaatagt cttatttagt aagaagttct
    13801 tagttaaatt ctcaataaaa acggtatttc catcttggta tatagtatat gttgggatag
    13861 ttgcagctag tgttacggca ccagcattta atcaactttt gattggcaaa attgcctttt
    13921 ggataggatt tgtatcttat atagttctta taccagtaat tttaaagaga gtatggataa
    13981 tcaaggaaat gcctgaacca tctttaccaa gtatagctat attagctgca ccagggtcac
    14041 tattattgtc tggatatatt aattcttttt cagataaaaa taatgtagta ttatacttgt
    14101 tattcatatt atcaattgta ttttatatta tagttttggg atatttaccg aaattattaa
    14161 gattgccttt ttatccgagt tttgctgcat ttacatttcc tatggcaata agtgcaatgg
    14221 gtatgaaact aatgaatggt tatttaacta aatcaggtca ttttataagt tggatttcat
    14281 atgcttctaa gttagaagag ataattgcta cagtgattat tttatatgtt ttagttttgt
    14341 ttattatata tcttagtaaa tctgaaacta catctaaagc taaataagaa ttattaaata
    14401 tgtgtattta ataggaatat tgatataaat atatgaagat aagataaacc atagaaactt
    14461 aatttagctt aattttattg gaggaaaaca aatgaaaaca aaagaagaat atttaaaaga
    14521 agttagaaac atatcagaag aatactttag aaaaggagag tttttttgct ctgaagcagt
    14581 tctccagaca ataaatgatg ctttaggcca acctttaagt ccagagataa ctaagttagc
    14641 atcaggattt ccgataggac ttggaaaggc acaatgtctt tgtggagcaa tatctggggg
    14701 agagatggct ttaggtatag tatatggaag agttcatggt gaagctatga atccaaagat
    14761 gtttgagcat gctaaaggtt tgcatgatta tattaaaaaa gagtatggtg caacatgttg
    14821 tagagctatt acaaagaaat gggatggtga taactttatg tctcccgaaa gaaaagagca
    14881 ctgtattaaa ataacaggtc aagttacaga gtgggttgca gataagctta tagaagatga
    14941 aaaacttaat atcaaatagt gtaatattag ttggtgaatt tataaagtta aatttactta
    15001 tagtataaga tacttgatat tctcgtattg atagaaaaat ttatagcata tgtatttaat
    15061 gaaatatact tatatttaga gtgtggttta atttcataaa tacatatgct attattttaa
    15121 gtatataagc aaatttattt aaataaatag aaacagttaa tttatatgta caaaactata
    15181 tatgattagt gtaaataaat acctatttta taatactcaa cataaagaca ccagacatga
    15241 ttagtattat accaataaaa atagcaagcc attctttttt gttcttcttt tctttaaaaa
    15301 atataatccc tcctaaagtg gctactatca cacaactctg agagatagaa aaacttattg
    15361 taactcccaa tgtattagtc gttatgaaca tacctaaatt tgctattgac cacaatatac
    15421 cagtaattaa attgaataaa acattttccc tatatacaat ctctttagaa aaatacttga
    15481 caataataga tgaagttatc aacataccaa cagattgagg taatataatt ctatatcctt
    15541 caattaaaaa tatttgatta gttacaacat ataatgataa aaatagagat gataataata
    15601 caactccaac acattttaac atatttttat ctgtagattt tttagtttga taatttgtga
    15661 gaagtatacc aagtattata cataatattg ataaagaacc aatcagtaac attttagaac
    15721 tggtccattc attaaacaag attacagcta ataatgtcgt aaaagttaat tgtgaaccat
    15781 ttgaaattgg cattacttta gatacactag caatttgaat tgctttaaac tgcattaatt
    15841 gtcccaaact ccagaatatg ccagaaatca tacttataca aaatgaaaaa aatgtaattt
    15901 caggatgtat aaccagagtt attatagtgg aaaatataaa cgcacttatg gatgtaccta
    15961 aaagctgttc atgaggagaa cttttcttta gatttgcaac tatgggcatc aatccccatc
    16021 ccattacagg aagtaaagca ataattattg tcagcattaa actatttcct ttcatattaa
    16081 atttagacag cttttaattt atcatctaaa attaaagtgt tcaatacata aaaattaccg
    16141 tatttaatca ggaaatatta gtagtttttt agaaaaacat tttccattaa aataaagaca
    16201 aagggaagga gtaaaattga aaatgttaac tgatatgatt caaaatcgtg aaattaaaat
    16261 aataaagttt ttattaaaag aaggtcatac aactgtaaaa gcaatagcag atgatttaga
    16321 tgtttcagaa aaaacagttt caaaatctct taaagaagta gaagcagtgc ttaaaaagat
    16381 aggtctttct ttaataagaa aacctaagat aggtgtttat attgatggag atttaaaaaa
    16441 aatacttcct tacctagata ataaaggaag tcaaattcct acaaccagag aggagagagt
    16501 tatctatata tattcagtac ttttaaagag taatgattat attactatac aacaactttc
    16561 tgatgaacta tatataagtc gtggaactat tgaaagggat ttagcagaag ttgaaaaact
    16621 gctaaaaaac gaagggattt ccatatataa gaaacctagt aagggaataa agttaaatat
    16681 aagtgaaaga gataaaagac aacttactgc taagtttata cataaatttt ggagtagaaa
    16741 ctggtatatt aagcaagagc aagaaagctt ttatcaagca tttgaaaaga tacaagcaga
    16801 tgtaaatggt attttttcgg aagatgattt atcaataatt attgatattc ttaggaattt
    16861 cagcagagaa agaaaatttg agttctctga ttatgaattt cagtcattag ttattcattt
    16921 ggcaatagct gtagagagaa ttagaaaagg cgaatatata gaagaaagtt tgtatactgg
    16981 taatatagaa aataatcaaa tgtcaaatac caattattta gcaaaacaat tagaagaaaa
    17041 attttcaata ttattaccaa aatctgaaat tggatatata aatttacatt tagtagcgtc
    17101 tgaccaacat agagatacta atgaactaaa aaatacttca actaatcctt gtgagacaag
    17161 tacaaataat atatcaaatc ttcgggaaat tataaaaaat acattgtatg agacagctta
    17221 tgacaaggat ttaattgatg gattgacttc acatgtacat tcatcaataa atagactgaa
    17281 atatggactt agtattaaaa atccatatgt aaatacaatc aaacaaaact tttctttatc
    17341 atttgagcaa gctttagatt taaagaaagt aattgaattt atatatgata ttaggataaa
    17401 tgatgatgaa tgtgcttata tagcattaca ttttgaagct tttagagaaa gaatgaaaga
    17461 acttccacaa aatattagag taattctagt atgcagtaca ggattaggtt catctaggtt
    17521 attgtctgca agaataaaaa aatactttcc aatgataaag attgaaaaag tactttccgt
    17581 acaagaatta gtatccagta aaattgatgc agatttagta ataagtacta tttatttaga
    17641 gttagaaaca attccaacca tagtagttag tcctatttta aatgaaagtg atataagaac
    17701 cttggaaaga aatatacaaa atgtttcaag ttgtacttct aagaagtata aatctttcag
    17761 aaacttaatc tttgaagaaa atatattgtt gaatattgaa gcttcaaact atgaagatgc
    17821 aattaagtat ataacagata acctaataaa aaaaggtttt gcaaaagaag gtatagctaa
    17881 aagtgcaatt gagagagaaa atttatctta cacttctttc caatcaatag ctacacctca
    17941 tgctaatcct agttatatta aaaaatctaa tatatctgtt gcaacgttaa aaaatcctat
    18001 tacatgggga aatgaaaagg tggaagttat tttctttatt tcattagaaa atgacaaaaa
    18061 cctagagctt gatgaaatat atgaagtttt ttatgacttt attgacaata aaaaaaatat
    18121 aaatgacata ttaagtgcaa cttccacaaa ggaagtgtac gatttgctag tggaggagag
    18181 tatatagtga aaatatcaga tgtattgaaa aaagagcaaa ttgtttttaa tcttgataca
    18241 aatacaaagg aagaaacaat aaaaaaatta agtagtatat ttgttgaaaa tgggattgtg
    18301 gacaatgaag aagaatatgt taagtctgtt ttggaaagag aagaacattc aactacaggt
    18361 attggtaatg gtattgctat tcctcatggg aaaagtaatg ctgtcaaaaa atcttctata
    18421 atatttgcaa agactaagca taagattgaa tgggatgctt tagatgaaaa accagtagat
    18481 agtattttct tattggctat aagcaatatt gataaggata gcaatcatct tgtactatta
    18541 tctaaattag caactaaact tatggatgat gacaatgtag atgcactaaa attagccaat
    18601 tcagaacaag aaataatcaa tatatttagt gaagaaggag agatgtagaa atgaaaagaa
    18661 aaattatagc agttacagca tgtgcaacag gagttgcaca tacatatatg gcagcacaag
    18721 cattaaagaa agcttcaaaa aagatgggtc accttataaa agttgaaaca caaggagcta
    18781 caggaatcga taatgagtta actacaaagg atgcacaaat tgcagaagta gtaatatttg
    18841 cagtagatac taaagtcaga aatgctgaac gttttgaggg aaaagaaata ctagaagtac
    18901 cagttaatgc acctataaag gatgcagaga aagtaataaa tgatgcacta aagttaattg
    18961 acactaaata aatggaggga aatgtaatgt tgaaaaagat agtctctgag ttaaaaaagc
    19021 atgtcttaac tggaatctca tatatgattc cattagtaat tgcaggtgct atgataatgg
    19081 cgatatctcg tgtaggtggt tctttctata atataccaga tatatgggat gctaaatatg
    19141 cagaaagtgc tagtagtatt gttagattat tacatgattt agatgggttt ggaggtactg
    19201 cattaggatt aatgtttcct gtaattgctg catttatagg attttctata gctgataaat
    19261 tagctattgt accaggtctt gttggaggta tgattgctaa agatataggt gcaggatttt
    19321 taggagcttt ggcagttggt cttatagcag gatatacatg tttgtttatt aaaaatcatg
    19381 ttaaattacc aaaatcagca gcttcaatag taccaatatt tatagtacca gtatttggga
    19441 cattaatcac tgttgtatta atcaattatg tgataggtat accttttgcc acattaaata
    19501 ctggattaga aaattggtta aatggattat ctggtacaaa tcagatacta atggcagcta
    19561 ttataggggc tatgatggca gttgacttag gtggaccagt taataaagca gctttaacaa
    19621 catcacttgc attattaaca agtggaattt atgcgcctaa cactgctgca atggtaggaa
    19681 tagttatacc accactagga ttgggacttg ctactatact agcaaaacaa aaatataata
    19741 aacagttacg tgaagctggg aaatcatctc ttataatggg tttaattgga gtaagtgaag
    19801 gagcaattcc ttttgcagta gaatctccat taaaagtaat accttcatgt gtaattggaa
    19861 cagcaattgc ttcagctatg gcagttggtt taggttcagt aaatgcaact ccaattagtg
    19921 gattttatgg atggtttaca gtagagaatt ggccaatgta tgtgttatca atagcagttg
    19981 gaacagtaat tgtagctgga ttagttatag tattgcgtgg aaatccagtg attgaggatg
    20041 atgaatttga agatgatgat tttgatgaag cagagtggga atcttaaaat ttaataacta
    20101 tttaagacgt gttataagct atattcaata tgaatcttat actaaataaa gtgttaaata
    20161 ttcagtttag acttaaatat aaaagataaa gaacagagga gaataaatga taaaagcaca
    20221 tattgttaat catacacact gggatagaga atggtatttt acttctggag atgcattagt
    20281 acttagtgaa caactattta cagatgtaat tgatgaatta gaacgtaatc cagatgtaag
    20341 ttttgtactt gatggacaac tttctatttt ggatgattat gtacaattac acaaagaaaa
    20401 aatagaaatc attaaaaagt taataaaaga agatagacta catattggac catggtttac
    20461 tcaaactgat gcattctttg ctagaggaga gtctatttta agaaacttaa tgattggaat
    20521 ttttgaaagt aaaaagtatg gtaagtatat gaaaattggg tatttgccag atacatttgg
    20581 atttaatgca cagatgccta tactattaaa gcatgtaggt cttgataata tattcttttg
    20641 gagaggtata catcttggta aacaggttca atcaccatat tttaagtgga aaagcttaaa
    20701 tggagaaagt tatatatatg cagtaaatat gccacatgga tatggaactg gtatgctttt
    20761 agagccttca ttaaagtatg ttaatggacg cttagaccca gctattgatt ttattaaatc
    20821 atatactgat gtagatgagg tgttaatacc ttctgggaat gaccagttaa atataatagg
    20881 tgatttcaaa aataaaatta aagcgattaa tgatatcgga aagtattcat atataacgag
    20941 ttcatatcaa gagtttttaa aatatataaa gtccatagaa tcactagaaa gctatagagg
    21001 tgaatttaga gaacctgtat tagcaagaat acataaatca attggttcta gtagaatgga
    21061 tataaaatta gcatgtgata aactagaaaa caagctgatt aaaagaatag agccattatt
    21121 agtaattgct aaaaagagta aaattgaaat aagcaatcag ttacttataa atacatggaa
    21181 aaaacttttg gaaggacaag cacacgatag tttagctgga tgtgtaactg atactgtaac
    21241 agatgatatt cttcaccgta taagagaagc aaatgaaata tgtgacagta ttgaaaatac
    21301 tattgtaaaa aagatttcag aagggattaa attatctaaa aatgatattt tagtattcaa
    21361 tacagaagct aagcgtttta atggatataa agagatacaa gttgtaagtg atagtaaaaa
    21421 tatttatttt aaagataatt tagatgctac tataattgaa gaaacttatg taaaaccgag
    21481 agaaaatgta ttagaagaaa cacctgctgg taatatattt attgaagaac caggatatta
    21541 tatattaaaa gtaagaataa atatacagct tcctgcaatt ggttataaag ttgttagttt
    21601 tgaattaagt gataaagaaa tggtgtcact tgataaatct aatgacactt ttatatctaa
    21661 tgataattat aagataatat atgaagatgg aagcttaaat ttacaactta aagatggaac
    21721 ttacatatca aactttttaa cattaaaaga tagtggtaat gcaggtgata cctatgactt
    21781 ttctccacta aaaaatgatg aggatataaa actttcgttt gataaagtgt atacagaaaa
    21841 agctttagga tatgaaaaaa tggtggttaa aggtactact ttactaccac ttacattaga
    21901 agatagaaaa aacaaatatc ttaatgggaa attaaatgta aaagtaagta ttgtcttatc
    21961 taaagaaaac ccattgatgg atgtaaaact ttgtgttgat aacacgatat ataatcatag
    22021 actacgtgtt catataaaaa ctgatataaa agataataaa aacatagcat cattgccatt
    22081 tggatatata acaagagaaa atggagtttt agacaactgg gaagatattt acagtgaaat
    22141 gcctatagat ttagaacctt tggaaagtaa tattacgctt acaaaccaaa atagaagttg
    22201 tactgtattt actagaggaa taaaagagta tcaacatatt gaagatgaaa ttgcattgac
    22261 cttacttgct actacagatg aactaggaaa gcctgattta ttatatagac caggacgtgc
    22321 ttctggagat acaactaaaa aaggtcatat aagaattaaa acagataaag ctcaaatgct
    22381 taaggaatta gaattttcat ttgcaatata tatggattct aaatcatttg atgaattgga
    22441 aattgcgaat ttaacccaca attatctaag agaatgtgta aattatcaat tacaggatta
    22501 caatttcttt ttatatcgta tagataataa gattcaaaaa tcaattgtca ataaaactgt
    22561 aagtagagaa ttagaaataa tatctttacc agaaaactat cttatatcag catgttatcc
    22621 ttcttattac agtaaagata aatttataat aagagtggaa aatccgagta gtaatagaat
    22681 gatattggat gagcaaatat ttaaaaatag aaatgggaaa attgtaaatg caatagaaga
    22741 tgtagaagaa gaacaggtat atgaaatagc accttttgac gttatttcaa tactattgga
    22801 tttataagtg taaaagtaag tttattgttt aaagtctgac agatattaat ctgccagact
    22861 ttttaatttg tataaatttg ggtaaatatt aatgaatggt tttagtattt aagtatgaat
    22921 ttagtattta atatagaatt tattgaagta gaataattag tttaacagga taagcagttt
    22981 aaataaagaa ttattatatt agagtgattt tatatataat ttaaaataat aaaattttaa
    23041 taa
//
LOCUS       DAEMIK010000055        22859 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEMIK010000055 DAEMIK010000000
VERSION     DAEMIK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.055"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(63..812)
                     /locus_tag="KPK87_001809"
     CDS             complement(63..812)
                     /locus_tag="KPK87_001809"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG2537961.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(879..1766)
                     /locus_tag="KPK87_001810"
     CDS             complement(879..1766)
                     /locus_tag="KPK87_001810"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2537962.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(1883..2884)
                     /locus_tag="KPK87_001811"
     CDS             complement(1883..2884)
                     /locus_tag="KPK87_001811"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG2537963.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(3309..4190)
                     /locus_tag="KPK87_001812"
     CDS             complement(3309..4190)
                     /locus_tag="KPK87_001812"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2537964.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            4513..5706
                     /locus_tag="KPK87_001813"
     CDS             4513..5706
                     /locus_tag="KPK87_001813"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG2537965.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            5912..7030
                     /locus_tag="KPK87_001814"
     CDS             5912..7030
                     /locus_tag="KPK87_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG2537966.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            7161..7406
                     /locus_tag="KPK87_001815"
     CDS             7161..7406
                     /locus_tag="KPK87_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG2537967.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            7393..8139
                     /locus_tag="KPK87_001816"
     CDS             7393..8139
                     /locus_tag="KPK87_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2537968.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            8424..9299
                     /gene="hslO"
                     /locus_tag="KPK87_001817"
     CDS             8424..9299
                     /gene="hslO"
                     /locus_tag="KPK87_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG2537969.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            9690..10490
                     /locus_tag="KPK87_001818"
     CDS             9690..10490
                     /locus_tag="KPK87_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2537970.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            11018..13258
                     /locus_tag="KPK87_001819"
     CDS             11018..13258
                     /locus_tag="KPK87_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2537971.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            13672..15207
                     /locus_tag="KPK87_001820"
     CDS             13672..15207
                     /locus_tag="KPK87_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2537972.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            15223..15984
                     /locus_tag="KPK87_001821"
     CDS             15223..15984
                     /locus_tag="KPK87_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2537973.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            16322..17191
                     /locus_tag="KPK87_001822"
     CDS             16322..17191
                     /locus_tag="KPK87_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2537974.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            17520..17771
                     /locus_tag="KPK87_001823"
     CDS             17520..17771
                     /locus_tag="KPK87_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG2537975.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            17883..18662
                     /locus_tag="KPK87_001824"
     CDS             17883..18662
                     /locus_tag="KPK87_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2537976.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(18797..19447)
                     /locus_tag="KPK87_001825"
     CDS             complement(18797..19447)
                     /locus_tag="KPK87_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537977.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(19444..19947)
                     /locus_tag="KPK87_001826"
     CDS             complement(19444..19947)
                     /locus_tag="KPK87_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2537978.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(19996..20511)
                     /locus_tag="KPK87_001827"
     CDS             complement(19996..20511)
                     /locus_tag="KPK87_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2537979.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            20791..21318
                     /locus_tag="KPK87_001828"
     CDS             20791..21318
                     /locus_tag="KPK87_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2537980.1"
                     /translation="MHEQIDCCERKKIKTINIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            21302..22705
                     /locus_tag="KPK87_001829"
     CDS             21302..22705
                     /locus_tag="KPK87_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2537981.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         8511 a         2755 c         3885 g         7708 t
ORIGIN      
        1 cgtattatta tatctatatt gtatatgttt aatttatatt gtgtattatt atatttatac
       61 tattatccca acatgctgtc catattataa aatcctggtt tttgcttaac taagtatttg
      121 gcagcagcta tagaaccact tgcaaagata ccacgagatt gagcttgatg gcttattgta
      181 agaacctcat tgtctccagc aaatagagca tcatgttcgc caacaatatt tccacctctt
      241 atagcatgaa taccaacttc atttggttgt ctttttgcac tacgaccata tctaccataa
      301 ttattttcaa cgtttggcaa aacctctttt atagcgttag caatcataac agctgtgcca
      361 cttggggcat ctacttttct gttgtgatgt ttttctatta tttctatatc aaatccttct
      421 agtaatttag ctgcatcttt aaccaatttt actaatacat ttacaccaag agacatatta
      481 tatgattgga atacaggaat aactttagcc gcttcacgta ttttgttcat ttcgtcatca
      541 ttatatccag tagtagcaat tactaaaggt gttttagtct ttaaaacgta agataagata
      601 ttatctaaat tagagtgatg agaaaaatct ataactacat cagcttcctc aactatagta
      661 ctcatatctt cataaatttt aaggtcgcct tcatagctat ccagacctct agctgcaaca
      721 caaaccaatt ctaattcatt atcttctctt atagtatcgg ctaaaatctt acccattttt
      781 ccatcataac cagttgctat aacttttaac ataatttcct cctatgatta agggctaact
      841 tttgaccaaa cttacttttt accatgcatt aatctttact atgcattaac ttttagtcca
      901 aagttaacta attctttctt taacacttct aaatttgctg ggtccatttc tgctagaggt
      961 aatcttaaat ctccaacatt gaatcccaat aaattcattg cagtcttaac tggtatagga
     1021 tttacttcta taaataaagc tgcgattaga gcatccatac caagttgtaa ttttctagaa
     1081 ccttcaatat cgccttcaaa gaatttagct accaaatcgt gagtttcttg agggcaaaca
     1141 ttagcaagta ctgatataac tccttgacct cctaaagata ataaaggaag tatagtatca
     1201 tcattaccag agtagatagc aaagtcatct ggaactaaac tagctatttc tgcaacctgt
     1261 cctaggtctc cactagcttc ttttacagca actatgttat caattttagc aagttcaact
     1321 ataaggctag gacttatatt tacacaagtt cttccaggga cattgtaaag tataattgga
     1381 agtttaacac tgttagctat tgtttcaaaa tgacgcttaa gtccagcttt attagcttta
     1441 ttgtaataag gtgttattat taatagagca tctactccta atttttcagc ttcttgactt
     1501 aagttaactg agtgcatagt atcattagaa ccacttccag ctataacagg aactctttta
     1561 ttaacttttt caacaacata cttaatagta gcaagttgtt cttcatcagt catagtatta
     1621 gcttcaccag tagtaccaca agatactata gcatcagtac cattagctat ttggtattca
     1681 actaattcac ctaatttgtc aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga
     1741 gcaacagcag aacctttaaa tatcatataa aatcaatccc ctctttttta tttttgtgtt
     1801 ttatattaca gttacttcta atgcttgttt ttgagttact attaatattt tacaaaatta
     1861 tatatttata caagtttgta tattatacta aatcgtatga taaagccatt tcagctattt
     1921 gaattgcgtt agttgcagca ccttttctta tattatcagc aacaacccaa agatttacac
     1981 cattatcaac actaaaatct cttcttattc ttccaacaaa tacttcatct ctatcatcaa
     2041 attctacagc agttggatat tcgtttttag caggattatc aactactaca acacctggag
     2101 cttcttcaag tgtttttact aaatcatcta actcaaatgg attgttaaac tcaacattta
     2161 tagattcact atgaccattt tttactggaa ctcttacagt agttgcagtt atttttaaat
     2221 cgtagttatc aagtattttc atagtttcat ttaccatttt catttcttct ttagtgtatc
     2281 cattttcagt gaatgaatct atatgaggaa gacagttgta agagatttga tgagggtaga
     2341 atccagtagg attaccgttc attcctttct ctaaatcttc aacaccttta acaccagaac
     2401 cagatacagc ttggtaagta gagtaaacaa ctctcttaat tttgtattta tcatcaagag
     2461 ctttaagggg aaccatagct tgtatagtag agcagttagg attagctatt atacccttat
     2521 gattttttat ggcttctgga ttaacctcag gaactactaa aggcacatct ggattcattc
     2581 tccattgact agagttatct actactaaga ttccatttga agcagctatt ggagcatact
     2641 tttcactaat acttccacca gcagagaata atgctatttg tatgcctcta tcagtaaatg
     2701 aagtttcctt taattcttct actatatatt ctttaccaca aaacattact ttagaaccag
     2761 ctgatttaga agaagagaat aaaaatagat tttctattgg aaaatttctt tcttctaata
     2821 cttttaaaaa tgttctacct accattccag tagcaccaac tattgctaca tttacttttt
     2881 tcatataaat tcaccccaat tttaaattat taaaacatta ttttatttta gttatacaag
     2941 taagatatgt taatattttt atatatccaa aaactttaat attatattaa caactttaat
     3001 tatatacaaa aaacgtacaa aggtatactt tgtacgtcta atttatcact caaagtaaac
     3061 cctgtagctc atcacctaca aaaataagtg acagtattac acatgttatg tgcaatccca
     3121 gaaaatatta ttgcaagaaa tattttcttc ggctgtaaat cctttttcca atcattaaca
     3181 cctgccagct actattggat actcttagtt acagctacct ctaccctagg ttaataggat
     3241 attaaaattt gattattcta ataataatac atatttaaga aaaaatcaac ttatttatat
     3301 aaatattgct aaaatttaaa tccaaaattt gtcaattctt gttttaacac attaagatta
     3361 gtttcttcca tttcagctag aggaagtctt aaatcaccaa cattaaaacc taaaatgttc
     3421 atggcagttt taacaggaac tggatttact tctataaata aggcagcaat taaagcatcc
     3481 atatcaagtt gtaatttttt tgagccttca atatcacctt caaagaattt agttactaaa
     3541 tcatgtgttt ctttaggaca tatgttagca agtacagaga taacaccact tccacctaat
     3601 gataatagtg gtagtatagt atcatcatta ccggagtata tagcaaagtc tttaggtact
     3661 aatttagcta tttctgcaac ttgagctaag tctccacttg cttccttcac agcaactata
     3721 ttatctattt tagcaagttc ggccacaaca gatggcttta tattcacttt agttcttcct
     3781 ggtacgttgt ataaaataat tggcaactta acgctatttg ctattgtttc aaagtgaagt
     3841 ttaagacctt tctcattagt cttattatag taaggagtta ttattaaaag accatccacc
     3901 cctaattttt cagcttcttg actcatatga acagacaaca tagtattgtt tgaaccagta
     3961 cctgctataa caggtattct cttattaact ttttcaactg tatattttat tacagcaaaa
     4021 atttcagatt cactcatagt agtcgcctca cctgttgttc cacaaacaac tagtgcatct
     4081 gtgccattct caatgtgata ttcgattaat tcacctaatt tttcaaagtt gacattatta
     4141 tcctctgtaa atggggtaac taaagcaaca gcagaacctt taaataacat ataatcaaat
     4201 ctcctttttt atctaaattt aattttttaa gtacaataat aattatagta caaattaatt
     4261 aattttaata ggtatagtta atatcttaac tgtttaaata agatatttat attatattaa
     4321 tattagaaaa attttaaata aatgttttat tctcaaaata tgcatattaa tttaataaaa
     4381 tataccaaaa tggaaataaa aaataaattt tatgagaatt taataactaa taattaatat
     4441 atttaggtta taataaatat gtgcgtaaaa tctaacaata agcaataata ccgtaaattt
     4501 aggaggaata atatggatac tttaaaggaa ctttttaaaa taggaagtgg accttcaagt
     4561 tctcatacta tgggaccaca aagagcagca gagagattta agaatgaaaa cccagatgca
     4621 gaaagtttta gagcaatatt atatggtagt ttggcagcca cagggaaagg tcatttaact
     4681 gactatataa ttgaaaaaac tatagcacct aaaaaagtcg aaattgtttg ggaagaagat
     4741 attataaaag attttcaccc aaatgggatg aagtttgaag ctttagataa agataaaaat
     4801 gtaacagctg aatggactgt atattctgta ggtggaggga ctatagcaga agaaggtcaa
     4861 agaaacagta aaagcaatag tatatatcac ttagatacta tggatgagat agtaaaatgg
     4921 tgcaaagaaa ataataagac tttggtagat tttgtgttgg aatgtgaacc aaaagacata
     4981 aaagattata taaaaactat aaaagatgct atgagaaaat ctattgatga tggactaagt
     5041 acagatgaaa taataccagg caagttatta ttaaaaagaa gagcaagtac attttataat
     5101 gcatataaaa aagataagag cttctcaaca cttgtatatg catatgcact agcagcttca
     5161 gagcaaaacg cttctggtaa tataatagtt acagcaccaa cttgtggttc tgctggagta
     5221 ataccaggaa tattctttgc aatgcaggat ttctataatt atgatgacga aaaaataatt
     5281 gaagcactac ttgtagctgg aataataggt aatataataa aaactaacgc ttcaatatct
     5341 ggagcagagg tagggtgtca aggtgaagtt ggagccgctt gttctatggc agcagcagca
     5401 gtagcatact taaaaggtgg aactatagac catattgaat atgcggcaga aatagcactt
     5461 gaacatcatt taggaatgac ttgtgaccct gtatatggat atgttcaaat tccatgtata
     5521 gaaagaaatg caatggcagc tcaaagagct tatgatgcag ctaattatgc attattaact
     5581 gatggctcac attctgtctc cttagaccaa gttgttgaaa caatgaaaga aactggtata
     5641 gatatgatgg ataagtacaa agaaacagct aagggtggac ttgctaaaca tttcttctct
     5701 tgctaaaaat aaaattttcc aataataatt cctctgtttt gatttatgaa acaggggaat
     5761 tattattaat ggtgaattat ttaataatgc aaaatatttt tatattcaat ttagcttttt
     5821 atttttaatt agtataacat gctatataat attttaatta taaagaaata tagaaatatt
     5881 ttttaataaa ttagaaattg gaggagatta catgagagag gcatcatatt tacaagaaca
     5941 actttataaa cataggaagt cattaaatcc tattgcagag attgggagaa aagagtataa
     6001 gacttcaaaa tatataagag attatcttga taaattagga attgagtatg aagtttattt
     6061 agaaacaggt atagtaggtg taatagaagg taaaagacct aaaaaagata ttgcttttag
     6121 agctgacata gatggtcttc atagtgaaga aggggtaaaa catttatgtg gtcatgatgg
     6181 acatgctact atattacttg gattaattga gtatttaaat gataataaag aagacttaaa
     6241 tgataatata gtatttatat tccaaccagc agaagaaggt cctgggggag caaaagcttt
     6301 aatagatgaa ggagtgctta gaaaatacaa tattgatgaa atatatgggc ttcatatata
     6361 tccagagtta ccagaaggtt atgtaggtac aagagagggc tattttatgg ctcaaatagg
     6421 agacattgac atagaaatag tgtctaaaag tggacatggg gctatgccac aaaatggtat
     6481 tgatggtata gtgatagctt caaactttgt aagctcactt caaactatag tgtctagaaa
     6541 tataagccca atagacaatg cagtgttgac tattggtaga attgaagggg gagctagaag
     6601 aaatataata gctgaaaata taaagatgga aggtactatg agatgtttca gcccagaagt
     6661 gtatgagaaa atgaagctta gagttagaga acttgctaga gggtttgaat tggcttataa
     6721 ctgtaaggtt aatataaata ttatagatga ttatattgct gttaaaaatg acaaggattt
     6781 gtatcaagag tttgtagagg ctattggaag tgatactgtt gtagagttag agccacttat
     6841 gatatcagaa gatttttctt attatcaaaa agaagttcca ggattatttt ttatgttggg
     6901 ttcaagaaac gaagaaaaag gatttgtaaa tggtcttcat aatataaact ttaattttga
     6961 tgaaaagata tgtgtaaatg cattgaatgt ttattcaaaa ttattaaaat acaaagaagc
     7021 aatagattag gaagataagt tataaagatt aaaaagcata aatacctaaa tttaaaaaaa
     7081 gtgtattaat aaatacccct tacatatttg tataaattta ttttaattga caaaaataca
     7141 aataagaagg ggtgattttt ttgaaagaaa aagtacaaag agaaaagaag ataactaatg
     7201 gcaagaatac taaaatattg acaaatgatg atataatgaa atatgagata gcctcagaac
     7261 taggtcttat ggataaagtt ggtgaacgtg gatgggcagg acttacagct aaggaagcag
     7321 gaagaattgg aggtatgctt acatcacgca aaaagaggct taaaaagatt gtggaggagc
     7381 agaataaaaa ggatgagtca atatagtgat tttgcgtttg tatatgatga acttatgaat
     7441 gaagttgatt ataatggttg ggttaaatat atagaagata taatagatag tgaaggtgta
     7501 aaagtaaaaa atatattgga attggcatgt ggcacgggta atcttacaat tccattgact
     7561 aaaaaaaatt acgatatagc tggtatagat atatctgatg agatgcttag tgtagcaaga
     7621 gaaaagtcag aaaaagaagg agtagaatta gttttacttc aacaagatat atcagagctt
     7681 gattttgaaa tatctgattt ggactgtgta ctttgtgctt gtgatggatt caattatata
     7741 acttatgatg atgatttgga aaatgtattt tcaaagacat atgaattatt aaagaaagat
     7801 ggaatattca tatttgatat aagttctttt tataaattag ccaacatatt gggaaataac
     7861 atgtatggag aaaatagaga agatatagcg tatatgtggc aaaattactt tgatgaagaa
     7921 gagaatctaa ttgaaatgga acttacattc tttattaaag atgaatgtgg aaaatttgaa
     7981 agatttgaag agatacacca acaaagagca tatactgaag atgagatagt tgatatgctt
     8041 gaaaaatctg gatttaagga tataaaatat tatggggatt ttacatttga caaacctagt
     8101 gaagaaagtc aaagaatctt ctttgtatgt aaaaaataag atatttttga ctatataaga
     8161 taattattaa aaataaccat gatataaaaa tatagaaaat gcagttttat agattaaaag
     8221 taaagtgatg atactttatg acttttattt tttaataatt ataaagttct atagataaaa
     8281 attaaaatat aataactatt gcttagtaaa attaaaataa aatgggtttt gcccaaggtt
     8341 aagatagaca agtatttata gattaaaatt aaggtagtaa tataataaaa ataagacaat
     8401 taacgggaca tggaggaata ttaatgagag attatgtact aagagcgaca tcaggaaatg
     8461 gtcaagttag agcatttgtc gcaactacta gaaatacagt agaagaagct agaagattac
     8521 atgaaactac aaaagtagca acagcagcac ttggaagaac attaacagcc acatctataa
     8581 tgggattaat gatgaaaaat gatagtgata aattgactgt aataataaaa ggtggaggtc
     8641 caattggaac tattatcgcc acatcagact ctaagggaat ggtaaaagga tatgtaggaa
     8701 atccacaagt agaggttgaa gactatccaa atggaaagtt aaatgtagca gcagcagtag
     8761 gtacagaagg tgttgtaaaa gttataaaag acttaggtct tagagaacca tataatggga
     8821 cttatccatt ggtaagtgga gaaattgcag aagactttac ttattatttt gctgtatcag
     8881 agcagactcc atcagtagta gctttaggtg ttcttacaaa agaagatgaa gtagagtttg
     8941 ctggaggatt tatagttcaa ttaatgccag atgcagaaga agagactatt gctaagttag
     9001 aggaaaatgt agctaaatta ccttctataa ctaacatgtt aaaagaagga aaatcaccag
     9061 aggatatatt aaatattgtt ttggatggtt tggaacctaa aatacttgat acctgtgaag
     9121 ttgggtttat gtgtgaatgt tcaaaagaaa gagtaaaaac tgctttggtt gcaattggta
     9181 aaaaatcatt ggctcaaata atagaagaag ataaaaaagc agaagttggt tgccagttct
     9241 gtaataagaa gtatatgtat agtgaagaag agttgcttga aattttaaag gaaatgtaga
     9301 ctataaaaat agttgtattg atataagtaa tgttatagta atatagtaaa gtcgtattga
     9361 tatagtaatt aaagtataat ttattaataa tagacatatt tatattaaaa aataagttta
     9421 ataaattttt aagcttaata aattttaaga attaagaagc tttctggctg taatgtttgc
     9481 aatgttgcag ttagaaggct tttttatttt attatagttc gtgtcaaaga ttgtatgaaa
     9541 caataaaata ttgtaaaata atttgtatat ttagacctga ttgacacaaa ggtttataag
     9601 atatttgagt tgattatacg aaattctatg aagtagtaac cctgtgactt taatcatggg
     9661 aggttcagaa gaaggagaaa attttaaata tggaaagtaa aacgaagtac ataggaataa
     9721 gtatattaat attattgatg atatttttaa ttgcaaagaa ttttaacgga aaacttgaaa
     9781 tacctattcc tgagagtaat agtagaaaat ttaaatcagc agcagtgttt tatcctcaac
     9841 atcaagatga tgaggtttta tggggaggaa gtgccattgt agatgctata aaacagtgtg
     9901 gtgttgataa tgtatatgtg gtattagtat cagatggttc tggagttaac gttttcaaag
     9961 ctaacacaaa atttagaaat ttaactagga agcaaaagga agaacttaga aataacgagt
    10021 ttaaatcagc tttgagagag cttggagtga aacctcaaaa tgtgattata cttgcagata
    10081 tagataaaaa ggaaggcaca cattatgaat taatggaaaa gactatattg gattttgaac
    10141 ataagtttaa aggcaatgtg acccatatag cccatcacta taagtatgat gaccatataa
    10201 tgcatagaaa gaatggggaa gttttaaaga aacttaaacg tgaaggtaag ataaaagatg
    10261 acttgtattt tgttaagcca aaatatgtaa agtatatacc aagaaatgaa agagctatct
    10321 ataaagcaat ggattttgat gactacaata aggttaagag tgcttgttat gaatataagg
    10381 ttgtaaattc aaagcgtaaa atgtatggta taggatatat ttcggcacat agttattttg
    10441 aacatcttct aaatgaccct gaacttacgt ctgtattaag tgaagattaa tttcttataa
    10501 gtagtaagtt atgtttgata agttgattgg aattttctat agttttgaag gtattggaaa
    10561 tttagtttta tttaaagatt taaagataga ggtttttagt gtttaaaatt ttaaagttaa
    10621 gttgaataat tgtatagaat tatttgtgaa agaagaaaca gtgtaaaaca tagtatctta
    10681 tttatggttt taaaatcata ggcaagatac tttttttatg taactaatac cattttgaga
    10741 ttattaactt aagaatttta tataatttaa cactcatata taatgatgat atttaggtag
    10801 aatagatgta attacgctta aataaaataa ctgtattttt tataaaaatc caaaccctac
    10861 actatactac cagataaaat ctacatgcta taatttgacc tgtgtttata aaaaaaggag
    10921 taatctaata aaatagtatt tcacttatgt ataactaaca actattatta cgattttctc
    10981 taaataattt aaagaaaaat aatgcaggag gtaaaatatg aacaatgaat ttataatttt
    11041 aaaaggttgc aaagagaaca atttgaacaa tatttcctta aaaataccaa aaagaaaaat
    11101 aaccatattc acaggggtgt cgggttctgg aaagtcatct attgtatttg agacaattgc
    11161 aaaagaatca caacgccaac taaatgaaag gtttagtaca tttgtaagaa gttttttacc
    11221 taagtatgga gaggttaaag ctgattgtat agaaaatttg tcaacaccaa ttatcataga
    11281 ccaaagtaga cttggtggaa attcaaggtc aactctaggt actattacag atgtaaattc
    11341 ctttttaaga gctttatatt caaggtttgg tagtgagtat ataggtaatg ccaatatgtt
    11401 ttcttttaat aatattgatg gtatgtgccc acactgtcat gggcttggaa aaaagctagt
    11461 tcctaatatg aaccaaattc ttgatatgaa taaatcatta aatgaaggtg ctatattgct
    11521 ttcagggttt ggagtgggtt cttggcattg gaatatattc gctgaatcag gattttttga
    11581 tattaataag aggatatgtg attattcaga agtagaattg gaaaagtttt tgcatggtaa
    11641 agcagaaaag attaagatag aaaatgctgg tcaaactaac atgacatatg agggattaat
    11701 ggttaaattt aatcgcctat acttaagtag agagggagaa atatctgaag ctactaagaa
    11761 aaaattgagt aaacttctta tagaagatag atgtcctata tgtgatggaa gaagacttaa
    11821 tgaaagagta tataggtctt taattaatgg ttataatata gcagatttaa ctagtatgca
    11881 aattgatgaa ttggctgaag ttattaaaag tatagatgag ccagaagcac aacctttaat
    11941 taaaggaata atagaaaagt taaacagtat catagatata ggtcttggat atttaacact
    12001 agatagagag acatcttctc tatcaggagg ggaatctcag cgtataaaga tggttaagta
    12061 tttaaatagt aatcttgttg atttaatgta tatttttgat gaaccaagtg taggacttca
    12121 tccaaaagat gtgtataaat taaataatct tttaaaaaaa ttgcgtgata aagggaatac
    12181 aattatagtt gttgaacatg acccagatgt tattaagatt gcagaccata ttgtagatgt
    12241 aggaccaaag gcaggtaagt atggtggaga agttgtgtat gaaggcagtt atgaaaatct
    12301 acttacaagt ggtactttga cagggaatgc tttaagtaaa tttcttccta ttaaagaaag
    12361 tgtaagagaa catagtggat atttagaagt taaaaattgt aataaaaaca atttaaaaaa
    12421 tgtatcgatt aagataccta aaggtgtatt gactctagtt actggagtag caggagcagg
    12481 taaaagtacg ttgattaagg atgaattttt aaagcaaaat cctagtgctg tacttataga
    12541 ccaaagccct gtttcagcaa attcaaggtc gagtttggca acatatagtg gaattatgaa
    12601 caacataaga agcatatttt caaaaacaaa tggcgtaaat gcttctttat tcagttcaaa
    12661 ttcagaagga gcttgtgata actgtaaggg ttctggaata gtagaaatga atcttgcttt
    12721 tatggagagt ataaagagta cctgcaatat ttgtgaaggt aaaaaattta aaaaagaagt
    12781 tttagaatat aggtttcaag ggaagaatat aattgaagtt cttgaaatgt ctgttttaga
    12841 ggcaattgag ttttttaatt taaaacagat taaaacgaaa ttacaatgta ttgaaaaaat
    12901 gggtattgga taccttactt taggacaaac tcttgatact ttgtctggag gagagtgtca
    12961 aagattaaag cttgctagtg aattaaataa ggaaagttca gtgtatatat tagatgaacc
    13021 aacaacaggt cttcatatgg ctgatattga gaattttata agtataattg aagagattgt
    13081 agataatgga aatacggtca taataattga acacaatata gatgtaatta aaagagctga
    13141 ttggattatt gagctgggac ctgaaggagg aacaaaaggc gggagagtaa tatttgaagg
    13201 tattccaaaa caattatgta acaataaaat atcattaaca gcaaaatata tagtataata
    13261 aataaaatat aataaaatta taatgatatt ttaataaata taatgggtat ataaaatgat
    13321 aattaaactt aacagtaata aataatgtga gaaatattca aatttaatta aataaagtgt
    13381 cagaaatttg tttaaatttt agtattttaa cggttctgac cttttttctt atttttatgg
    13441 agaatatcga ggatggagtc tctaatagtt acttttttgt gtctacttgc aagtttgatt
    13501 aaaaaatggc tatacacact tgtgacttca gtcatgggag gttcagatta taatcttaat
    13561 atataatttg tgaaataatt tgatgacgat ttgctgatgt ataaacaata aagattaact
    13621 acattaaaat ttaaatcttg ttaagtaaaa cttataaaag gagagagatg gatgataaat
    13681 gatattctaa ccttattatc tgaaagaagt gattttttta taaagctatc catagaacat
    13741 ttgatgattt ctattatttc tataataatt gcgattgttt tgggtggtat aattggtatt
    13801 attgtaagtg aatttcataa ttcttctaaa cctatattgg gaactatcaa ttttctatac
    13861 accattccat ctatttctat gctaggattt ctgataccat tttccggaat tggaaatact
    13921 actgctgtta ttgcacttac catttatgca ttattgccaa tggttagaaa tacatatact
    13981 ggaattacta atgtggataa aaatattctt gaagcagcta ggggaatggg aagtacagat
    14041 tttcagatat tgtataaaat aaaacttccg ttagccctac ctgttattat gtctggtatt
    14101 agaaatatgg tgattatgac aattgcatta gctggtatcg cttcatttat aggtgctgga
    14161 ggcctaggtg tagccatata tagaggaatc acgacaaata acacaataat gacaattact
    14221 ggaagtatac ttattgctct attagcttta atatttgatt ttattttagg gctggtagaa
    14281 aaattttttc aacgaaaaag aaaaaagaaa aataggaaaa aagtttttgc cattctagga
    14341 agccttattc ttatagtaat tatctttacg tcttatttat ttaatccttc aaagactcaa
    14401 actattcata ttgctactaa acctatgact gaacaatata ttcttggtga gatgttggat
    14461 attttaattg aaaaaaacac aggtctaaac gtggaaatta cacaaggtgt aggaggggga
    14521 acatctaata tccagccagc tatggagaat ggagaatttg acatttatcc agagtataca
    14581 ggtacaggct ggaatatggt acttaagaaa aatgaattat atactgaaag tatgtttagt
    14641 aaactacagt cggaatacaa aactaaactt aaaatgcaat ggactggtat gtatggtttt
    14701 tctaatagct atgcattagc agttagaaaa gaagttgcag ataaatacaa tttaaaaaat
    14761 ataacggact taaaagatgt atcttctaaa ttgacatttg gagctgaata tgacttttat
    14821 gagagagagg atggatatac tccattgtgt aatacatatg gactgaactt taaagataca
    14881 gttgatttag atattggatt aaaataccaa gctatcaatg aaggtaagat tgacgtaatg
    14941 cctatattta caactgatgg tcagtatagt gtttcagatg ttgtagttct aaaagatgat
    15001 aaaaatttct acccatctta tcaatgtgga aatgttgttc gtttggaagt attagaggaa
    15061 aatccagaac tatcaaaggt atttaagatg gttggagata ttttgactga taaagaaatg
    15121 gctaaaatga attatgacgt agaaaacaac aatttagaac ctagagaagt tgctcgtgag
    15181 tttctgaata agaaaggtct tttataaatt ggaggaaata taatgacacc tattatacaa
    15241 tttaaaaata ttaaaaaaca atataatgat aaaactataa tagataatct taatttagat
    15301 atagaaaaag gagagttttt gactgtaatt ggttcgtctg gttctggtaa gacaacactc
    15361 ttaaaaatga ttaatggtct gattttgcca gatggaggaa atattttgat aaaccaaaca
    15421 gatatcaaaa acgaggactt aattaaatta agaagaagaa taggatattg tgtacaggga
    15481 agtgttcttt ttcctcatat gactgttgaa gaaaatattt catatgtacc aaatctccta
    15541 agtaaaaaga ataaattaga agtaaaaagt gctgtaaaca agtggatgga aatagtggga
    15601 ttgccaaatg atatgaaagt gcgttaccca tcagaattgt caggtggtca gcaacaacgt
    15661 gtagggattg caagagcttt agcttcttct ccagaaattt tattaatgga tgaacctttt
    15721 ggtgcagttg atgagattac acgaaagcaa ttacaaaaag agataaaaga aattcataaa
    15781 aaaacaggag ttacgattat ctttattacg catgatattt atgaagcttt gattttggga
    15841 acaaagactt tggtattaaa tcatggagtt gttcaacagt atgatactcc tgagaatatt
    15901 ttaaatactc ctgctaacca atttgtagac cagttattga atataagaaa atctataata
    15961 gatgatgata atttaaaaga ataaatttaa atttaaaatc aaattgttgt gataaaaact
    16021 attgatttag agctaacttc aagttgtata cttgaagtgg aaaagttaga ttaaggtata
    16081 gaagggcatt tagcagttgg ctgtagacga ggtaactgct gaaggtgctt tcaataaatg
    16141 gctagaacct gtatctgttg aagattataa taaattgaaa taagaagctc aatatacaga
    16201 gtagataggc acttcaaata gaagtgtctt ttgtgtgcca taaaataaat caaaatatta
    16261 gcaaagaatg tgaaaaacaa aataaaataa tatgctatct ttaaaatagg agggatgcta
    16321 tatggataac aatttgatta ccattgaaaa aactttagat tatatagaaa aacatctttt
    16381 agatgagatg ttagatttaa atgtaatttc taaagagata ggctattcaa aataccattt
    16441 acatagaatg tttacatcag ttgtcggatt tagtgtacat aattatatac aaagacgtag
    16501 acttactgaa gcagcgagaa tgctaatttt tacagaaaaa ccaattattg agattgcttt
    16561 aatctcgggg tatgatacac aacgctcttt ttcaagaggg tttaaaaaaa tgtttaaacg
    16621 tagtcctaat ttttttagaa gacatcaaga ttttttacca ctacaattaa aatttgatat
    16681 tcataataga aaaaaattac gtggagatat ggttttagat gttaaaaaag ttaattgtgc
    16741 agatataaat attgttggct attctggtaa agtaagaaaa ggatttcata ttattggaaa
    16801 atgctggagg gatttgcata aaaataaaga gatgatagaa aataaggtag atttaggttt
    16861 tttaattgga gttagtgatt atagcaattt taaagagatt gagaatactc ctacatttac
    16921 atacattgca ggtgctcaag tagctacttt tggcaaatta cctaatggaa tggaaaaatt
    16981 cgtattaccg aaaagtaatt atattgtttt tacttttatt gggaaaaatg aggatagtat
    17041 gcaaaatgtt gtagaatata tctatcaaga atggttccct aattcaagtt gccagtttaa
    17101 tgaaaataat ttatatgact ttgtaaaata tggagaaaaa actgatgaga atggtttgag
    17161 tgaaattcag ttttgggttc caatcattta aaagaaggta ttatgtataa agtagatgga
    17221 tttatatagg caaagtttta cagcagtatg tatttataaa taatttatag tttgtaggaa
    17281 agaaataatt ttactataag attatgataa aatacacaat actatgacaa aatagtgtta
    17341 aataatagat ttaattttgc ataggaaaag ttctataatg gtgtgtattc ataaataatt
    17401 tataatctgt aggaaagaaa tagttttact ataagattat gataaaatac acaatactat
    17461 gataaaatag tactaaataa tagatttaat tttgcaaagg ataaggtgtt ttaataaaaa
    17521 tgaatgtaga aaacgaatta cttaaaaacc tagatagatt gcatacaaca gagttaggcg
    17581 ttgtaagaat taagaaaaat ctttctttgg agacaaatga tgtagtgaac tggtgtaaaa
    17641 ctaaaataga atctccaaat gccattatta atagaaaggg aaaaaactgg tatataagtg
    17701 tatatgattg tattataaca gtaaacgctc acagttatac aattattaca gctcataaag
    17761 aaaagaaatg aattgcaaaa tacaaaggtc aaaagcattt tccactgttt gtagtaccta
    17821 tgaagaagta tataaaataa tattactttt gaataataat ttaaattttg gaggttgaaa
    17881 aaatgaatga tactgtaaaa gaaaaattgg cagagtcact aacttctgat actactgata
    17941 ttttacctta tttgccttat ttattacaag atttttggga attaggaagt tcaccagaac
    18001 aaatgttaca attatttaga aagcatatac cttttaatga ggatacaaaa gttttggatt
    18061 tagcttgtgg aaaaggtgct gtatctgtga agcttgcaaa agagttgaat atacatatca
    18121 aaggtattga tttgatacct gaatttattg attatgctag aagaaaggct attgaatata
    18181 aggttgatgc tttgtgtgat tttgaaatga atgatgctaa tagttctgta aaaattgagc
    18241 atgattatga ttgtgtgatt tttggagctg taggggatat tttaggtaat tataaagaaa
    18301 ctataagtaa gctattacct acaataaaaa gtaaggggtt cattttaatt gatgatgcat
    18361 atgtagtaga taaatctaca aatgaacagc ttaattttga gagggtgtat cccacttaca
    18421 atgagtggtg tatattgttc aaagaattag gtctgaagct aattgaatgt ataccatgtt
    18481 tagaacttga aaatgatgtt gaagaggaaa tgaattggat tataactcgt gcaaatgagc
    18541 taattaaaaa atatccttct caaaaagata tgtttgaaca atatgttatt agccaaaaaa
    18601 atgaatatac tgatttacag caggaccttg ttggagcaac ttggatttta caaaaagaat
    18661 aagataaata ttagactaga taatcaatgt tatagtgaat tatcccatgt ctagtgaata
    18721 taaataacat aaaaaaccgt caagatgaat agtttattcg tgcttgacgg tttttagtat
    18781 ttgttgaagt aaaattttat ttctttagta tatcactttt agtcaatcca tttgatttag
    18841 cttgagtatt ccattgatga ttccccataa gtttatatag gaatctaggg aagttaggtg
    18901 aaataaaaat agtacttttg ttggaatcat ctatatgtat attatcacta agtatatttt
    18961 ttgaaagttc ttttaatgca attcctaaat cttttttagg tcctttaccc aaaggtacgg
    19021 attctagtga accaaacatc tccccagctc caattccaat accttgaacc catgtaaagt
    19081 tgattttttt acaccaatgc tctatcattt gaattgccaa atgattatgt tcaccttcat
    19141 tgaatccaca gttaactatt gcatacactg gaataggttt tttattttgt gtttttaaaa
    19201 atttttcaca tttctctaaa aaatgaagta aatgagatgg aataccatct acataaagag
    19261 gagatgcgat tattaatcta tcacaattaa gtatggcatt aagagcgtca ggatacttag
    19321 atgtgctatc tactttaaat gtaaaaactt tattatcatt ttgaataagt tgttttaatt
    19381 cagataaaaa atactgtgaa ttatttttat ttacttttgg actaccattt atcattacta
    19441 tattcatatg gattcacctc ttatatcttg tatattttca tagaaacaaa cagagtgttc
    19501 ttttatattg aaatttatag catttccatt tactaatttc gtagcagttt ctttttcagc
    19561 ttctgtaata caatctccat agaatatagc ttttaaattt atctgtttat catatctgct
    19621 tttatgacgc atcatattat cttttattac aaagtatgga agaatataat taatgctttt
    19681 atcaagtacg tttttgacaa aagggctaaa ccctccgtaa cagcatctgc ttattataat
    19741 aattccatca gagtcattta taagtttacc catgttgctg taataatctt ttacaacaca
    19801 ttcagaagga gttttaatcc agcaaccaaa acaaccaata catttgtgga tttcttttgt
    19861 gtttggaagt atttttatat tgtcatcaag tttgggtaat aacttttcta attctttgtc
    19921 agttaaatca tgtattaaaa gcctcataaa gtccatctcc ttttattatg tatgtttact
    19981 tctaaaaatt atatcttata gatttttcag taaatcttta caccaattaa taacaaaatt
    20041 ataatagtca actccataaa tcattgttgc atacatatat gtatattggt caacacaatg
    20101 attttcttct gcatcatttt tttgtttact taatttattt agaagtaatt ctacttcagt
    20161 tattaacatt tttaagtttg tttcaattat ttctttgggt agatacttgt aaaagaatat
    20221 attaacaagg tggtcttgct ttgttttaga aaaatttata ggtttcttta accattcaag
    20281 aaatacttct ttaccagtat tagttatgga atatagtttt tttaatttgc taccatctat
    20341 aacttcgtga tagtgtatat atccttttgt ttctaatctt ttaagtgcag gatagatact
    20401 tccaaagctg gcgtcaaaaa agtacgaggt actttctgac attatttgtt ttaagtcgta
    20461 tccacttaat tctttttcca ttaaaaatcc taaaataatg tattctaaca tattacctcc
    20521 tggtatcaat tagatatata tcgattcgat atatttatta ttacagataa taaatcttac
    20581 gtcaagatgc tttttaaaat aagctaaaaa gaattaggag ttattgataa atatgcaaaa
    20641 aaattaatat aaaaggtaat taaatatcaa tgaagattta aaaatagtta aatcaaagta
    20701 agagtctaat atataaaatt tatagtaact ttatggttta aatataaact tgtgtgctat
    20761 tgacaaagta taaaattagc agtaaaataa ttgcatgagc aaatagattg ttgcgaacgc
    20821 aaaaaaataa agacaataaa tattaaagga gagaatatga gagagctatt aaaatgggtt
    20881 tccattataa atagatacaa tacaaattat attgacagga aattggcgga tattggaatc
    20941 aataatagcc aatattttta tgtattacac atatgtaata atccaggtat tacacaggac
    21001 actatatttg agaaaattct tgtgaatcct agtaatataa caagagcttt ggcaaattta
    21061 gaaaaagaag gctttataac aagagaacca ggtgtaaagg ataaaagaac atggcattta
    21121 tatcctacag aaaaatcttt agaatgttat gatgaaatac taaaaataac aaatgggtat
    21181 gttgaggagt tgttgagttc atttgagaaa gaagagcaag agttgtttat gtcaatgtta
    21241 aagaaagttg catttttggc tattgatatg aatgaagaag caaaattgga ggcgaaggaa
    21301 aatggaagaa aaatataagg agccattgga atatgaaaag gtttcaaagt tattaactaa
    21361 gtacgcaatt cctagtatta tagcaatgtt agtaagtgct ttatataaca ttgttgacca
    21421 aatatttatt gggcaaggtg ttggtgtatt gggtaatgct gctacaaatg tcacttttcc
    21481 actaacaaca atatgtactg caattgcatt attgttagga attggaggag cgtctaagtg
    21541 ttcattggaa ttaggtgcta aaaattcaga aaaagctaca aaagcagcag gaaattctat
    21601 atgcttgatg gctgtttttg gaataagttt atttatagta gtatcaatat ttttaacacc
    21661 actattaaaa ttctttggct ctacatcaga aatactacct tatgcacaca catatacaaa
    21721 aattacatca attggattac catttcttat aatgtccact ggaatgagta agttgatact
    21781 tgcagatggg agaccgaaag cttctatgtt ttgtatgtta ataggagcag ttattaatac
    21841 aattttgaat ccattattca tttttgtttt taatatggga attgctgggt ctgctttagc
    21901 aactgttatt gggcaactta tatcttttgg aataagtgtt cattatataa agaattttca
    21961 acatattaaa ttacataaga aaagttttaa attagatagt ataatttata aacatatatt
    22021 tgcgctaggt tcaagctcat gttttaatca attagcaatg actgtaacac agatagttat
    22081 gaataataca ttggcatatt atggggctag gtcagtttat ggaagtgaga ttccacttgc
    22141 ttgtgtcggt atcattatta aagtaaatat gatttttata tctataatta ttggtatatc
    22201 acaaggtatg caaccaatta tagggtataa ttatggggct gaaaagtata atcgtgtaaa
    22261 agaagcatat aagtttgcag tgggaagtgc aactattata tctgttattg cattcttatg
    22321 tttccaattc ttcccaagac aaattacgtc tatatttggt aacggaaatg agacttattt
    22381 tatatttgca gaacattatt ttagaacttt cttgttctta acatttataa atggtgttca
    22441 aatagtatca tcaactttct ttacatcaat tgggaaatca gtgttgggac tttttacttc
    22501 tttgactcgt caaattttat ttttaattcc attgatagta attttaccaa tgtttatggg
    22561 aattgatgga gtaatgtttg caggaccaat agcagatgca gcagcggcaa tggtatgtat
    22621 atactttgca gttcatgaaa tgaaatcatt gactataaag caaaataatt tgatgagtaa
    22681 ccataataag attgcagagg tataagacaa tatttaagaa gtatagaaaa ttaatttaaa
    22741 aaatataata aattaattta ggaagtgtag aaaattaatc taaagaagta taataaacta
    22801 atttaggaag tatagaaaat taatctaaag aagtgtaata aactaattta ggaagtata
//
LOCUS       DAEMIK010000056        22852 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEMIK010000056 DAEMIK010000000
VERSION     DAEMIK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.056"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(31..1128)
                     /gene="dinB"
                     /locus_tag="KPK87_001830"
     CDS             complement(31..1128)
                     /gene="dinB"
                     /locus_tag="KPK87_001830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG2537982.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(1518..3023)
                     /locus_tag="KPK87_001831"
     CDS             complement(1518..3023)
                     /locus_tag="KPK87_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG2537983.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(3284..3634)
                     /locus_tag="KPK87_001832"
     CDS             complement(3284..3634)
                     /locus_tag="KPK87_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537984.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(3857..4048)
                     /locus_tag="KPK87_001833"
     CDS             complement(3857..4048)
                     /locus_tag="KPK87_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2537985.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(4166..5080)
                     /locus_tag="KPK87_001834"
     CDS             complement(4166..5080)
                     /locus_tag="KPK87_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2537986.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(5214..6710)
                     /locus_tag="KPK87_001835"
     CDS             complement(5214..6710)
                     /locus_tag="KPK87_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2537987.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(6990..8480)
                     /locus_tag="KPK87_001836"
     CDS             complement(6990..8480)
                     /locus_tag="KPK87_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2537988.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            8947..9147
                     /locus_tag="KPK87_001837"
     CDS             8947..9147
                     /locus_tag="KPK87_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2537989.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            complement(9327..10205)
                     /gene="speB"
                     /locus_tag="KPK87_001838"
     CDS             complement(9327..10205)
                     /gene="speB"
                     /locus_tag="KPK87_001838"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG2537990.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNILNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISNKIK"
     gene            complement(10195..11046)
                     /gene="speE"
                     /locus_tag="KPK87_001839"
     CDS             complement(10195..11046)
                     /gene="speE"
                     /locus_tag="KPK87_001839"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG2537991.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(11076..11489)
                     /gene="speD"
                     /locus_tag="KPK87_001840"
     CDS             complement(11076..11489)
                     /gene="speD"
                     /locus_tag="KPK87_001840"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG2537992.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(11576..13051)
                     /locus_tag="KPK87_001841"
     CDS             complement(11576..13051)
                     /locus_tag="KPK87_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2537993.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            complement(13689..14342)
                     /locus_tag="KPK87_001842"
     CDS             complement(13689..14342)
                     /locus_tag="KPK87_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2537994.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(14587..16452)
                     /locus_tag="KPK87_001843"
     CDS             complement(14587..16452)
                     /locus_tag="KPK87_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG2537995.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKNSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKTADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(16457..18877)
                     /locus_tag="KPK87_001844"
     CDS             complement(16457..18877)
                     /locus_tag="KPK87_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG2537996.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(18891..20333)
                     /gene="glgA"
                     /locus_tag="KPK87_001845"
     CDS             complement(18891..20333)
                     /gene="glgA"
                     /locus_tag="KPK87_001845"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG2537997.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRFDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(20362..21492)
                     /gene="glgD"
                     /locus_tag="KPK87_001846"
     CDS             complement(20362..21492)
                     /gene="glgD"
                     /locus_tag="KPK87_001846"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG2537998.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(21494..22654)
                     /locus_tag="KPK87_001847"
     CDS             complement(21494..22654)
                     /locus_tag="KPK87_001847"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG2537999.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         7221 a         4104 c         2313 g         9214 t
ORIGIN      
        1 aaagctagtg ttatttcatt taaacaaaac tcacatttgg atactatcca tgtattcaaa
       61 taatgtaata tctttataag tttctttttt atcacttagg tttgatattg taactcccaa
      121 tagcctaatt tgttttccat taacctcaac atttctcaac aaattatcta caccacttct
      181 tatatcatta tattcgtcaa tatagtgttc taaactaagg cttctagtta tctttgtaaa
      241 gtcctcatat ttaattttta atgttaatgt ttttccaaac ttctcagagt tttttaatct
      301 atgacatact tcttcacaaa gttcatcaag aatgttgcgt gtttcttctt catcaatatc
      361 caaattatga cttaatgtag tctcagcccc aacagatttt ctaaccctat ttggttccac
      421 tggtctatcg tctattcctc tagcaaattt atataattca taacctcttt ttttgaatat
      481 attttcaagt tcaaataaat ttaaacacct taaatcatat ccagttttta tacctaaatt
      541 tttaagagta tttgaagtaa cttttccaac accaaaaaat ttattcacag gtaatttatc
      601 taaaaagtcc tgggcatttt cttcggttat tacagttaac ccatttggct ttcttaagtc
      661 tgatgcaatc ttagcaagaa acttattgta tgacacacca gctgaagaag taagaccaac
      721 ttctctaaat atatcttttt ttatttgctt tgctatctca attgatgaat caatattttt
      781 tttatttttt gttacatcta agtaagcttc atctaaagat aatggttcaa ctaaatccgt
      841 gtatctataa aatatatctc ttatttgctc tgacacactc ttataaacat caaatcgagg
      901 tcttacaaaa actgcatatg gacacctatt gtaagctaca gctgatggca tagctgaatg
      961 gataccaaat tttctagctt cataagaaca cgttgcaacc acacctcggc tttgtggatt
     1021 tccaccgaca ataacaggtc ttccttttaa ttttttatta tctctttgtt ctatagatgc
     1081 ataaaaagcg tccatatcta tatgaattat ttttctataa tgtttcatta ttatcacctt
     1141 ttaaagtata aatagattaa ttttatttat attacttttt aattatacca aacttatgtt
     1201 tctttttggt attttttcat tattttcttt atatattcat attttccata aataaaacca
     1261 tctataatcc attaacttca atataattta taaaaaatcc ttaaaaacac ttgcaaaaac
     1321 tatataaaag ctttacaata ggtattccaa ggaactaaat ttaaaattcc ttatggataa
     1381 cagtatatat atttaattta tgggatgttt taaaaacaaa agactgtctt atacaagaca
     1441 gcctactgat tttattataa ttgtaatgga ctgctcaaac tattacttta aaacagccca
     1501 tattttttta tataatttta actatagtaa agattttgtg aaggatattc tgggtcacaa
     1561 gttacatcta taccaagctt tctaaatgtt tgttcatcat ctctatttaa tatagtagta
     1621 gaatgagctt gacacccttt taataatgct aacttttcca tagcaaattg tgcagttgga
     1681 ttagttacag cacaaatact aagtgcagtt aacacctctt ggcaactaag tgaaacattt
     1741 ctactagcta aagtctttgt ttttaagttt atgataggtt ctaatattac aggtgatatt
     1801 aagtgcatat catcagatat attggctaaa tgtttaatgg catttaatac tactgctgca
     1861 gttccattca ttaaatcaga acccttacca gttaatattg taccatcttc taattctaat
     1921 gcaactacag gacaagtatc attcttgtcg gcactttctt tcaatttatt actgtagttt
     1981 ctagcaggta atacaacatt tctatcttct ggctttaagt taagctcttc cataatcatt
     2041 ttcattctat cataagcacc tttatcaact tgtccctttt tatactcaca ggcagtttta
     2101 aagtatcttc ttattatttc ttctatagaa gcttttctta ttacttcatc atctattatt
     2161 ccaaagccaa ctctattaac acccatatca gttggagatt ggaaaattga ttcttttcca
     2221 gttatctttt ctattatttt tttaagcact ggaaataatt ccaaatctct attgtagttt
     2281 actgacattt caccatacgt ttcaaggtgg aatgaatcta tcatattcac atcttttaaa
     2341 tcaactgtag ctgcttcata agctatattc aaaggatgtt ttacaggtac attccaaact
     2401 gggaatgtct caaacttaga atatccaact acattacctc ttttattttc atgatacaac
     2461 tgacttagac aagtaccaag ttttccacta ttaggtcctg gtgctgtgac tactactatt
     2521 ggctttgtag tttctatata ttcatttgct ccatatccct catcactaac tatagtctca
     2581 acatctgacg gatatccctt tgttggataa tgcttataaa ctttaattcc tctacgttct
     2641 aatttattta taaatacagt tgtagcaggt tgtccttcaa atctagtaat tactacacta
     2701 tttacatcta gttcataacc tcttaaatca tctattaatc ttaaaacttc catatcataa
     2761 gtgataccaa agtctcctct tattttgttt ctttctatat ctcctgcata gacacaaatt
     2821 acaacttcaa ctttttcttt catatgttgt aatactttta tctttgcatt ttcatcaaat
     2881 cctggaagta ctctttttgc atgaaggtct cccatcaatt ttcctccaaa ctctaaataa
     2941 agtttgtcaa aattatttac tctttctaag atatatttag actgctcttc taaatatttt
     3001 ttgtggtcaa atcctacttt catcttaaca cctcactaaa ttaaatttct tgttgataac
     3061 agtatattta tatatatttt tttagtaaaa catcttataa aaaaatgtta atactttttc
     3121 actcaaagta atattataca attaagatta atttttttca agatagtttt atggtaaata
     3181 agatatacct atataactaa taaaataaga atattatatt tttatatata agtataatat
     3241 tctcacctta gcattaattt atttcaatat tttcatatat cttttattct aaattcaata
     3301 cttttctaat atcttctata ccttgataaa cttcttgttt agaaaaattc atagcttcta
     3361 attcttcatc agtcttttcc ataagcatag tataaaattc tattgctata tactgcatat
     3421 tttcactatt tacactctta gctttatcaa ataaaatttc ttctgcttcg tttacatttc
     3481 cctgaaaaac taatcttttt aaagttataa gtataacttc ttcttctgta ttatacacta
     3541 aatctttttc ttcatattca ctgtattttt ttcccaataa aagtcttgca accataagtt
     3601 ttatctcatt gaaaatctta tccactatag ccatttttac atctcctata aataaatttt
     3661 attttctttt taattatatc agactttatc gtaaaaatta ttatatgtaa atgaaataat
     3721 tcaaaaataa attttaacaa taaaaaaaag aaggtagcgg gggatttccg actaccttca
     3781 aacactagat tgtgattggt ttggtatttt atttataccc accataagct ttattaaatc
     3841 atctaaattg atttttttag catccacatc ctccatcaaa aacgtcacca cattcacaaa
     3901 gtattaataa taatattaag aaaggtatcc atgcacataa gtccatttca gcccaacaat
     3961 cttggaatgc taagaataag aagaataata ttattatcca ccaagcacat ccaccaaatc
     4021 cgccttcacc aaaaaatcta tccaacataa tatgttacct cctagtcatt ttgtatgtgg
     4081 agtttcagct cctaaaatat actatgttta aggggtaata tttgctaatg atttttttaa
     4141 atttttttag atatttttaa aaaaattatt gattcacact caatgtctta ccatcactat
     4201 tgataactac ctctccatta gaagtcaaaa atgtttttat attcaatctt tccaatattt
     4261 ttaaaacacc ttcctctggt tcttttttct ctgaacatgt aataacagca tattggggtg
     4321 aaatagactc taaaaatgtt tcagttaatt tgtcatatct tccatgatga ggcattttaa
     4381 caaaatcata ttttaaagta tttccattta taaactctga caatctttct tcctctgcat
     4441 ctcctgcaaa taaaaactta tgtgctccat agcttatatt agtgattatt gaataatcat
     4501 ttgaactctc gtacttgctt tttgaagccg gatgtatgtt tatttctgca ctattgatta
     4561 caatattcat attatctttt agtagcactg gttctatatc agctttttta agtgaatcaa
     4621 tatattcatc atattgctta ctatctttct tataatttgc ttgtataata ttctttactt
     4681 taacactaga taacacacta tctactccac ctatatggtc cttatctaaa tgagttaaaa
     4741 ttaaatagtc aagtgtattt attccttttt cttttaacct atccactatt tgttttccat
     4801 ttttatcttc tcctgtatca atcattatga ctttgttacc tatttcaacc aatatacagt
     4861 cagactttcc agtacttaat atagtagtct taaatgcagt attttttgtg ctactctgaa
     4921 tattattttg actcacatca tttttgtcac tttttaaata agattcattc gttgatttta
     4981 tcttactatt agtagttata tttgttacaa gcaataatat tactattata gataatgtag
     5041 taactaaaaa caggatatct tttaatacta ttttcctcat ccaaaagctc ctataaattt
     5101 tatttattac atatttatag tatagcaacc ttaactctgt aaaatacaca tataaaaagg
     5161 gcaaatttta ggcatatttg tggcatgata aaaaaatgac tgtaccaaaa caattatctt
     5221 tctaaattta catcttcaaa ttctttaatc ttatcattag ataaaacact ctttttagta
     5281 gattgtccat tttttctatc aaaagtattt ttagctttta cacctgataa aaacttacct
     5341 gctccattaa tacaaccttc ttcacacatc attccttcta taaaattacc ttctaatctt
     5401 cccaatttag ccattgtcat tgtcttttta atttgtcttg gaccagatac ttttataggt
     5461 tcaaaatcaa aactaagccc cttttcagca atatagttct gtactgattt ggcaagtcca
     5521 ccagatgctc caaatcccct tccaaaaatc gaaccatcat ttatatcttc atttgaacat
     5581 tttgataaat ctatattaaa tgcttcaaat aatgccatta gttcttcaaa agtaagtaca
     5641 taatctactg aatcttttaa acttttctct gttgcctcac tctttttagc tgtacatgga
     5701 cctataaata caactttagc atcagaatct atttttttta ctaatcttcc tgttgctacc
     5761 ataggagaca ctgtagatga tattctatct ttttcatctg ggaacatctt ctctatatac
     5821 ttaaaaaatc ccggacaaca tgaatttgtc atgtaattat ctcctgattt tattctttca
     5881 ataagttcat tgctttcatg aactgtaact gcatctgctc cacaagaagc ttctatcata
     5941 tctacaaatc ccagtgattt tatagcattt tttaactgac catatgtaat attactatct
     6001 atttgacctg ttatagctgg agctactatt gcatacatat tttctttctt tgcaagtttt
     6061 tttgcaacag gtgctatcaa actcttatca gatattgctc caaatggaca tgctgacata
     6121 catgctccac aatttataca attatcttca tgtatcattg ctttcatagt gtttctatta
     6181 aaatccaaag ctcctgttgg acaaacgctt ttacaaggtc ttacaacttc tgatatagca
     6241 tcatattgac atgaactttt gcacattcca caagccttac ataaatcatg atttatatat
     6301 gctctcccat ttatataaga aattgcccca aatttacatg cttccttaca tctatgagcc
     6361 aaacaccctc tacaaagctc tgtaactgta aattcattta ttggacatct atcacatgct
     6421 gcttcaacta tatatataat ttgctcatct gtgtccaaac taactgaatc tggatttata
     6481 tctccatatt tactatttgg agttaaactt aatgcaagtt gcgccctttc aagaactact
     6541 tctctctctt tataaacaca acatctatat ctcgcctttt ctcctttaat tatttcatat
     6601 ggaatttttt ctatattttc ttttgttaaa ttttcttcaa tagatagtct tgctactgct
     6661 gttaaaactt catgttttaa cctttttagt tgcgtttcaa attcaaacat aagtaatcct
     6721 ccctataaat cacacctttt tgctatattt ctactattat gtatattgta ttttttattt
     6781 atttctataa attattataa cataggcata tactttgtta agttatatac aaaatattat
     6841 atatgtaatt tttattttaa gtgtatataa tttttatttt agttgttcaa taattaaata
     6901 tataaataaa aatagagttg tccaaaagtt aaacctcaac ttagaacaac tctattaaca
     6961 atatgtattt ttgttgcttt gtattatctt tatttttcta aatttatatt actaaaatct
     7021 tttatctttg tatttgctaa gacacttttc ttagttgatt gagaattttt tctatcaaaa
     7081 gtggctttaa cctttgcacc tgataagaat ttaccagaac catttataca accaccttca
     7141 cacatcattc cttctataaa attaccttct agctttccta aattagccat agtcatagtt
     7201 ttcttgattt ctcttgaacc agaaatcttt acaggttcaa aattaacacc aatacctttt
     7261 tcagcaagat aattttctat agcttttgta agaccaccag cagctccaaa acctcttcca
     7321 aaaattgatg catcatctat atcttcattt gaacacttag ccaagtctat atcaaatgcc
     7381 tcaaataatg ccattatttc ttcaaaagta agtacataat caacagctcc ttttaattct
     7441 ttcttagctg cctcactctt tttggctgta catggtccta tgaatacaac tttagcatct
     7501 ggttcatgac tttttataaa tctaccacta gctatcatag gagacactgt agaagatatt
     7561 tttcctacat gatttgggta tgttttttct atataaccaa caaatcctgg acaacaagaa
     7621 tttgtcataa aagcttctcc cttttccatt ctttcagcaa attctaagct ttcatgaact
     7681 gtaactgcat ctgctccaca agctgcttct aatacatcaa caaatcctag tgattttata
     7741 gcatttttta tttgaccata tgtaacattt gcttcaactt gacctgttat agctggagct
     7801 accaccgcat acattttctc tttttgaact aactttctag ctacaggagc tatcaaactc
     7861 ttatcagata ttgctccaaa tggacatgct gacatacatg ctccacagtt taaacattta
     7921 tcctcatgta tcatagcttt catattttct ctatcaaatc ctaaggcatt agttggacaa
     7981 acactcttac atggtctaac aacctctgag atggcatcat attgacaagc ttttttacac
     8041 attccacaag ccttacataa ttcatgattt atataggcca ttcctccgac atgtgttata
     8101 gctccaaatt tacaagcttc tttacatcta tgtgctagac atcctctaca aatatctgtt
     8161 actgtaaatt catttattgg acaactgtca catgcagctt ccaaaatata tataatttgt
     8221 tcatcttctt ctatatccat tgtatctaaa tctatcttat cttgacctgg ttgtaacccc
     8281 atacttacat ttactctttc agatattata gctctttctt tataaacaca gcatctgtat
     8341 gtaggtttct caccctttat tatttcatac tgaatattat ccattgcttc tctggtaagg
     8401 ttatcttctt ttccaagtct agctattgca gttaaaacat catgtcttaa tttgtgtatt
     8461 tgtgtttcaa attgaaacat tttaatacca cccccgtaaa tgattttata cgataccatt
     8521 ataacacaat atgtcctttt attaatatat acataaaata ttatatgttt aaattttatt
     8581 ttatcgaata tatatagtgt ttttttgttt tattatataa tattattgat atttctgaaa
     8641 tttcactatt tttattttta cttttcaatc taatatataa tattttttat tatatatttt
     8701 aattttactt tttacttaca tacctttttg ttacattttt tgatattaaa tttattatct
     8761 acatttacat tttcgtcaaa ttttacaata cttctcactg tataatttaa caatttttat
     8821 taaattctaa aagtaattaa tttttttaaa aattttattt gcttttattt aaaatttatg
     8881 ttatattaat agaacagact tattttttta gtttgtattt tttaataatg cggaggtttt
     8941 cttataatga aaaacggaat agtaaaatgg tttaacaatg aaaaaggatt tgggttcata
     9001 tctgtagagg gagaagatga tgttttcgta catttctcag ctatacaaaa tgatggatac
     9061 aaaactttag aagaaggcga aaaggttagc tttgatataa ctcaaggtaa tagaggacct
     9121 caagctgaaa acgtaaatag aatataatta tttaatttaa atatatatat tttagaaaag
     9181 gagctatcga gggctctttt ttgttgttta ttatatttat ttttaattac aaaaataaga
     9241 agttacttta gaaaagcgtc aaacttaaac taactttttt aaaataactt cttatattat
     9301 aatatatact atttatataa aatactctat ttaattttat tagatattat taagcatagc
     9361 tctcttaaga ttttacaagc tataactgtc gatacacctg ttgtatcgta atcagggctt
     9421 aactctacaa tatcacaacc aactatattt atattagagt tttttataat cttaaaaatc
     9481 tcttgaaact ctctataatt tacaccacca ggttctggtg tacctgttcc tggaaacaca
     9541 gatgcatcca atacatctaa atctatagtt agataaatat tctttccatt tagtatatta
     9601 actatatttt caaacgtatc tattcctcct atttccatgt atgtgtgttt ttcttctgta
     9661 gcaaatttaa attcttcttt tgtcccagac cttataccaa attgaaatat tttattatca
     9721 cctacaatat cccatattct tttaattact gttgcatgag aatttttact attattatat
     9781 tcttccctca agtcagtatg ggcatcaaaa tgaattacat atatatcatt gtacttttca
     9841 tgtactgctt taaaggctgg taatgtaact aaatgctctc ctccaatcat aaagggtacc
     9901 tttgaatctc taacaatctt atatgtctct tgataaattt cttttaagac ttgttctgta
     9961 cttccaacac taatttctaa atctccataa tcacatatat tataatcctc taaatccaaa
    10021 tctaaaaaag gactatatgt ctcaagaccg taaaattctt ttctcataga gctacttgca
    10081 aatctagccc caggtctgtt agaagtagtt ccatcaaatc caacaccaaa tactataaga
    10141 ttagattctt cataattttt gtccatactc ataaaagtgt tcatatgata gaaattattc
    10201 ttcatctagc atctccttca cgtattgagg taacataaac gaacctaaat gtatatcact
    10261 attataatat ttagttttta aacttaattt ctcccatcca tttctatctt gattttttac
    10321 tggattaagt ttttttgaag caaatccaaa caaccagtga ccagatggat atgttggtat
    10381 atgtgcttga taaacttctg atattggaaa tatctgtttt aattttttat tagctctttt
    10441 catctcttta gcattaaagt caaaatacgg actttcactt tgatttacta aaattccatc
    10501 atcagtcagt attctataac aatccttata aaaatcagtt gagaaaagcc cttcacctgg
    10561 tcctattggg tcagttgaat ctactattat caggtcatat gatttatcat gtgcacactt
    10621 aacaaaatta acaccatctt caaaatataa actcactctt ttatcatcta atttacatga
    10681 gcatatatcc atatactcct tagatacatc tacaaccatt ttatctattt caaccatgtc
    10741 aatcttttct atttgaggat aacggcttaa ttctcttact gtacctccat cacctccacc
    10801 tatgactaat actttcttta tgtttaaatt tgtagccata ggaacatgag ttatcatctc
    10861 atggtatata aattcatctt tatatgttac catcattaat ccatctattg ttagaaattt
    10921 tccaaattca tcactatcaa acacatctat cctttgaaat tgagactttc cttcaaatag
    10981 atgtttattt acttttatag aaaatcttac attttctgtc cattcttctg tataccaaag
    11041 ttccatataa taatcctcac tttctcttgt atgttttata ctgcttctgg tttatgagta
    11101 atcttaccta aatctttttt tgaatatttt ttaatttttg tagttaagcc tcttggaatt
    11161 tctgtagact cgcttctttc agactttaaa agttgctcta ataattcaaa acttttccat
    11221 gggtctatat caccacaagt aaaaaaatct gctgctgcat agccatattc aggccatgta
    11281 tgtattgtca aatgagattc agcaattata acagctcctg aaacccccca aggattaaaa
    11341 tggtggaata cagaatccac aattgtcgct tttgcattta atgctgaatc attcataaaa
    11401 ttttctataa gacgtgggtc tttaagtacg ttttcatcac aattatagta ttctactagt
    11461 atatgtcttc ctagtgtttc aaatttcatt tcaaccccaa ctcctttata cttttaataa
    11521 gttctatttg ataaactcta cttaacaaat acttgccctt gcaaaataaa gtaatttatt
    11581 ttattactag tattctatct aagtttttat cttgtacatc tgtaagatac gcattgctat
    11641 tttttaagaa aataatatac tccataattt cttctgttat caattctcct ggagcaataa
    11701 tcggtatacc tggtggatat gccattattg attctccaca aattctatct atacatgaat
    11761 tgatttctac actttccttt ggtgcataaa aagcttctct tggattaagt ttaataattg
    11821 gatttatttg aaccatatta aattcattta atgttgattc ttttctataa atctttgcaa
    11881 ttatagaaag tgcctttact aatctatcta tatctgattt atttgttcca atggacacaa
    11941 gtgccagtat attatatgaa tctcccaatt ctacttgaat tgaaaaattt ttatataata
    12001 aatcgtatac ttcaaatcca gtcaaattta attcttttac atttatacac aattttgttt
    12061 catctataaa tttaacacct ttttgtttca atatttctgt agataaaact tttattccat
    12121 caattttgtt taatttagat tttgcatacc tagatagatt taatgctttt gacagttgct
    12181 tttcaccatt ctcaactaga ttaactctag caccatctat acttgccata agcaaataag
    12241 atgctgatgt tgattgtagc atattgattg attgaagaac cttttcaaaa cttacattat
    12301 ctctattcaa taagagtgct gaagattgtg tcaatgctcc tccagtttta tgtatactta
    12361 cagctaccat gtcagctcct aaacttattg cagaaggagg taaatctaaa tgaaatggaa
    12421 aatgtgcacc atgagcttca tcaactaaca ctaaaacatt atttttatgg catagttcta
    12481 taatactctc aaggtctgca caaactccat agtaagttgg attaaggaaa aataatgctt
    12541 ttatattaca atctttttct atctctgttc taactttttc aagagtaata tttaaactta
    12601 tacctagttt ttcatcaaat tctggttgta taaatattgg tattcctcca caaagtatta
    12661 acgcatttat tactgattta tgtatatttc ttggaagtaa tactttttca cctggtttta
    12721 taacactcaa aatcattgca tgtattgctt gagttgtgcc attcgttata aagaatgcct
    12781 catcagccat atatgcttgt gctagcaaat cttgtgcatt ttttataatc ccatttggcg
    12841 cagatacatt atccatagtt ggtgatgaat ttatatccat tttcatgaga ctttcaccta
    12901 gatatttatt taatatagct acacctctat tttttacatg tccaggcaca tcaaaacaag
    12961 ctatatcttt ctctgagtat tcctttaacg actctaataa tggagcacac tcatagctta
    13021 attcaactgc atttgcaaac tcgctatcca tgatttcaaa ttcatctcct ctctatattc
    13081 aaatattttt aatatttatt atttattatt tctatttttt atttttactt ttattaaact
    13141 catgatattt attatattaa catcccagca tttttacaag tgtttttttg aagttactca
    13201 aaaacatata cattagctat atcaatattt ataaactttt taattttttg ttgtttatat
    13261 taattttttt aaaacttagt atttataaac tttcttattt tttattattt tggttttttt
    13321 acttttttga gttttagtaa gcttttgagt ttactatttt taatttataa gtattttatt
    13381 actaaacttt tatattattc ttatttcaca tatcaaaatt cttggtttag ttggactaaa
    13441 taattttcct atttttgtat tttaatttta atataaaatt tattaaattt tttaattgat
    13501 aaaataaatt ctatatttat gatattaaaa ttctataagt tcattgattt atatatgtta
    13561 atttaaatac attattctat cgtagtgcct tagagttttt atttgtattt aaagttaaat
    13621 ttattcttgt ttttccttta atatttaata gtaaaacaac aaaattataa aaaaaataaa
    13681 gagaaacttt ataaaagctt ctcttttttc atctctgcta taggtatcgt tttccaatgt
    13741 tctctcaaaa ctctttcaac attttctaaa tcattctccc tcatagattt tactacttca
    13801 atatgttgat tattgctgta tgtcaacatt ttatataact catcttcatt atcactagta
    13861 tatgactttg acataaaatt atatcttaaa gagttcaata gatttactaa cacctgatta
    13921 tctgatttag ttatgtatat atcgtgaaat tcattactta attttaaata gctattgtaa
    13981 tttttatact ttatagagac cgccatcatt tctgttaatt cttccatttt tactaggtct
    14041 gattcagtta aattttgtaa tgctgaagaa ccggctaacg catccaatac tccaattacg
    14101 gaataggtgt ctaatttctc cttttcctga aattctttta ctacaaatcc cttattaggt
    14161 attttttcta gtaaattttc acctgctaac ctagttaatg cttctctcgc tggtgttcta
    14221 ctaactccga tagatttaca aatctcagat tcagtaattc tctgattagc ttcatattct
    14281 ccattttgga ttttctttga tatatagtca tatacttgag ttgtaagtgg atctaccttc
    14341 ataagacgtt cctccatatt aagattataa aagttaaatc tccctacaac tttggtagag
    14401 agttcagttt tatcttacta atttatataa tatcacaatt atgatatttt ttatacttct
    14461 ttgtaatatt ttgaaatact aaaattctta tgataattta tacaaaaaaa gtagaaaaag
    14521 ttttgataaa acaaaactct ttccacattc accttatttt ctatttattt ttctaaaaaa
    14581 agatatttaa tttactctaa aaattctaaa tgatttagag tctaaatcta ctttcaactc
    14641 attattttca atttgtatag tactatttgc atttttaaga ctatacttta taggtatttc
    14701 ctcaataaaa ctactctcta tacataatga tatactttcc acattttcac ttctatttac
    14761 taaaattaac attctatcat tattttcatt aactctaata aaagaaaata cagaatcatt
    14821 attagtatat ataaattctg tatttccaga acttaaaagt ttattcttat ttctaatttt
    14881 tataacatct ctataaaaat taagcatatc ttcgtcttca tttttccatg gataagttct
    14941 tctattatct gggtcaacac ctccacaaag acctgcttca tctccataat atatatatgg
    15001 tactccttca aatgtcatct gtatagctac agcaagcttc actaaatcta catcatcatt
    15061 taattccgtt ttagctcttg taacatcatg tgttccaatt aaatttaaat tagacctaaa
    15121 tgattctttt ggataatttt ctttaatctg catatatcta ttacaaagtt catatgaatt
    15181 aatttctcct tttaagaaag aaaacatgtt atttctaaaa ggataaccca taacagaatc
    15241 taattcttca cctaataaat aacttcttct ctgaccatag ctaatcttat ttgatgcatc
    15301 ttcccatact tcacctataa gaattgagtc atcatctgcc gtttttaatt ccttttttaa
    15361 ctctcttata aacttagttg gtaattcgtc tgcaacatct agtctccagc ccttaatacc
    15421 catatttacc catttattta taacgctatc attgtcatat attatataat ccatatagct
    15481 atgttctaat tcatttatat ttggcaatgt cttaacatcc caccaacttt tatatttttg
    15541 aggaaattct tcaaacatat accatgaata atatggagat tctttagatt gatatgcccc
    15601 taaactatta tagtttccat acatattaaa atatttacta tctgctccag tatgactaaa
    15661 aactccatct aatactattg atataccttt ttcttttgct ttatcaataa gttctttaaa
    15721 tgtatcttca tccccaaaca ttgggtcaat ttttttatag tcacctgtat catacttatg
    15781 attactagag gcttcaaata tagggcttag atatagtata cttacaccta atttttttaa
    15841 atatccaagt ttattgatta tccctcttaa atttccacca tggaaatccc atcttataac
    15901 atccccttga ctattcttta tgtacatagg tgtatcttcc caatttccat atatgaaact
    15961 atttttttta ggattatcaa ccttaccact acgatttcca ttattgaatc tatctacaaa
    16021 tatatgataa agtattcctt ctttgtacca tgttggtact ttaaaatctt tgtaaacagt
    16081 gatttgatat ttattaatat cactataatt atattcacaa gaatatccat tatctctgtt
    16141 ttttccataa aataaagtct ttatctgacc atccatttca acatcaactt taaagtaata
    16201 aaaataaaca cctgtaccat caaacttttc aatcttacca caaaagcact ttccaagttt
    16261 atcattatct aattcttcat ttaaagttat tgttctcatt tcttcttctc tatttatttc
    16321 tagggaaata gacttgatat tgtatccctc atttacttta acacaaatgg tcaattcctc
    16381 atcaaatttt aaagctccaa acggagcctt aaaattttta tcccaagagt tatattctat
    16441 tacactttgc atttaatcac acccttttaa accaaatgtc ttttgcatac tcagaaattg
    16501 ttctatcaga agaaaaaaat ccagagttag ctatattaac taaagacatt ttattccatt
    16561 tttctttatc tctatataac ctgtttatat ctgcttgagc ttgaccataa ttttcaaaat
    16621 ctctcaatac aaaatactca tcattgtaag tagtgagtga attataaata cttcttcctt
    16681 cttcgccaag attaggaata aatccattta ttaaatcatc tactaccctt tttatatcca
    16741 tattagaatg atataggtca agtgaagaat atccaccata tttattataa tttagtactt
    16801 gttctgcact aagtccaaat aaaaacatat tttctttacc aacctgttca catatctcta
    16861 catttgcacc gtcaagtgta gccaaagtta tagcaccatt catcataaac ttcatattac
    16921 ttgttccaga tgcttcctta gttgtagttg aaatttgctc actaacattt gctgctggta
    16981 ttattatttc tgctaatgat actccataat tctctaaaaa tactactttt aacttgtcct
    17041 ttactctaac atcattattt atagtacttg caacagaatt tataaattta attatacatt
    17101 tagccaaata atatcctggt gctgcttttg cgccaaatat aaaagttctt gggtcaagat
    17161 tcaaattagg attatcaagt aattcatgat acatatgaag tacattaaat atattcagta
    17221 actgtctttt atacgcatgc aatctcttaa cttgaacatc aaatatcgag cttgggtcta
    17281 catttaaatc gtatttatct tttataaact ttgctagatt tgctttatta ttgtatttta
    17341 tatcatcaag tctttgtaaa acagaggaat catttttaaa tttttcaata ttttttaatt
    17401 gtaatgtatc tttcttccat gaatcaccaa ttaaatctgt aattaaattg ctaagttgag
    17461 gatttgaact tataagccat cgcctatgtg caattccatt agtcttatta ttaaatttat
    17521 ttggttcatc ttgatagaaa tcttttaata cctctgtttc aagaatatga gtatgaagct
    17581 tagcaactcc atttacacta tgacttgtga caatacataa gtttgccata ttgatattgt
    17641 cacagtctat tatactcatt ctttttattc tatcttggtc atatccttta ttatttaatt
    17701 cctctacata tcttctattt atttcttcta ttatcatata aattcttggt aatagttctt
    17761 tcatcatact tacaggccat ttttccattg cttctgacat tatagtatga ttagtatagg
    17821 acactgtttt tttagttatt tcccatgctt catcccaaga taaattttct tcatcaagca
    17881 aaattctcat aagttctggt atgcataagg taggatgtgt atcatttata tgaattgcta
    17941 ccttatcgaa taaattatta atatttaatt tgttcttttt atattttctt attatatctt
    18001 gtatccctgc acttacaaaa aagtattctt gtttcaacct caacaactta cctgcatagt
    18061 ttgaatcatc aggatataat acctgtgata tttcctcagt ataatactta tgttttaaag
    18121 cctcttcata gctaccagaa taatttagag catttgaggt aagttcacca aaatctctct
    18181 ttggtatttc actcttaaac aatcttaaag tatttataca ttggttttgg tatccgatta
    18241 ttggtatatc ataaggcatt gccattacag gaaggtagtt tttatgaatt acttttaaat
    18301 gactaccttc cttgattaat tctacctcac caccaaactt caccattgtt gcttcatttg
    18361 gtcttacagt ttcccatgca tatcgtcctt ctgtaagcca gttatcaggc acttctacct
    18421 gataaccatt tacaaatttt tgttcaaaca gaccatattt ataccttatt ccatatcctt
    18481 gaccacttat gttcaaagat gccatagaat ctaaaaagca agctgctaat ctaccaagtc
    18541 caccatttcc taaggctggg tctacttcag cttcaattaa atcatccaag ttgattccta
    18601 attcagataa accttctcta acttcttctt caatatttaa atttaataaa ttagatttta
    18661 actgtctccc tattaaaaat tctaaactaa aataataaac ttcttttttc ttatctaact
    18721 ttgtagcaac ccattttttt gctatctcat ctttcaatag actacataat acatttaaca
    18781 attgttcatc agtagcttcc ttgatagatt gtgcatataa gctatacatt tttacttcaa
    18841 atttttgctt gaatttcttt ttgcttattg ttaccaaaaa aaccactcct ttaatcacat
    18901 atgtctctat atgtttctat atactcctta gctgattttt tccaactatt atctgttttc
    18961 attgcatttt caactaattt aatccatttt tctttgtcct taaatacttt gattgcattt
    19021 ttcaaacaga aaaacatctc atgagcattg taatttttaa aactaaatcc attgccttct
    19081 ccagtaaatt tattataagg tgttactgta tctctaagcc ctccagtctc tctaactatt
    19141 ggtaaagaac catatctcat agctaacatt tgacctatac cacaaggctc aaataatgat
    19201 ggcattaaga acatatcact tgcagcatat atctgttgag ccaatgatgc attaaaagta
    19261 attctagctg atactttatc tgaaaaatta gaatcgtaat agttgaacat tgattgatat
    19321 tgatgttctc cagttcctaa aacgacaatc tgtaaatctt ctctcataag ttctggcatc
    19381 atgtatgcta ttaaatcaaa tcctttttga gaaactaatc tcgaaactat acctatcaac
    19441 ggaatagatg aatctttttt aagtcccaat atatcttgta atctcaattt attttctact
    19501 tttttgtcaa ttgagtctac atcataatga acaaatatat ctttatcttt tgcagggtca
    19561 tttaaatcat aatctatacc atttaatata ccttttaatt ttcctgactc ttttctaagt
    19621 aatccatcta attgttcacc ataaaaagat gtttgtattt catttgcata agtaggacta
    19681 acagttataa ttttatctgc aaaatttata ccagctttca taaaatttat tgagccataa
    19741 tactctatat ctccattatc aaagtatttt tgtggtaagt ccaatatatc atataaaaca
    19801 tgcttatcaa atacaccttg atattggaga ttatgtatag tatacatagt ttttatattt
    19861 ttatacccct caagggttgc atatctttct tttagaatta atggtaacat ccctgtatgc
    19921 caatcattta tatgtattac atctggataa aaatcaaatc tacttatagc ttctaaaact
    19981 gcatctgtaa agaaagtata tctttctgca tcatctccat atccatagat actctttctt
    20041 tcatcttctc ttctaaaata aaattcatta tctataaagt aaaattttac accatctaat
    20101 tccatttcta aaagaccaca gtattgattt ctccatccaa cacgtacact aaacacagct
    20161 atttctttta gttgatcttt taaataactt ggtatagttg aatatttggg cattattacc
    20221 cttacatcta caccttcttt ttttaattct ttaggcaatg cataagaaac atctcctaat
    20281 cctccagttt ttgcaaaggg ccaacactct gctgttacat aaaaaacctt cattagtcat
    20341 tcccccttct aaaaaattta tttatatatt tgtattcttt tccactacaa gaggatattt
    20401 ttcatctcct cttaattctt tcccatttga tacactcgta tttttatcta atattgcatt
    20461 atatagtttt gcatttgact ttattacaca attttgcatt actattgagt tctctataac
    20521 tgttcctttt tcaacattta cctttctaaa tatgatacta tcttttacag taccttctat
    20581 taaacaacca gatgctataa agctattttc tactttagca ctatctgtat aaattgtagg
    20641 tgattcattc ttttcttttg taaatatctt tctttcagaa tataataatt catctgcaac
    20701 ttctaaattt aataaatctt tgctcatttc aaaatatgat tcaacagaat ttacacattt
    20761 tagatatcct gtatattcat aggctcctat tttcatgttt tctgcttctt caataataaa
    20821 ttcttctaaa taacaataat tgcctttatt tacaatgtta tatatacatt caacaaaatc
    20881 ttcacgcttc ataatatatg tctccataca tatgtttgct ctagggaact tacctaaatt
    20941 atttcctaca tttattaccc tattgttact atctaaattt agagttaagt taccagcaaa
    21001 atctttattt gcattattta catttttata tattatagtt atatcattat gagatttttt
    21061 atggtattcc aatgcttgag aataatttaa attgcatagc atataacttg gagctattaa
    21121 aatatattct tcttcactct tttctatata atctagatta gctaagatag tatatatatc
    21181 accttctcta tgtgagtaag tatttctata cttagtgttt tctggactaa aaattgatag
    21241 gccacctttt tttctactta aatcccaatc tttaccacta cctatatggt ctgttaatga
    21301 tctgtatttt tcttttgcga atattccaac tttagtgatt cctgaattta tcatattaga
    21361 taaagcaaag tctattattc tatatcttcc agctattggt gtagatgcta ttggtcttcc
    21421 atagttaagt ttgtttattg ctgggtctcc ttttttgttt aaatttatta tccctaaaca
    21481 ttcatttctc atcttaaaga cctccttcta ttaatacgca tctaggttcg atattttcat
    21541 actcagaaac tacatttatt tcattattta cattctttat ctgaacatta tcatttatag
    21601 ttgcacactc acctatcata gctttttcta tagttacatt ttcacctatt acaacatttg
    21661 gcattattac tgaatcaatt actttacttc ctttaccaac aacaactcca tgtgataata
    21721 cagagttacc tacttcacct aaaattctac aaccatcagc tatcattgat ctatgaacat
    21781 ttgcattttt acctatatat tgtggaggca ttgccatagt atttgtatat atcttccact
    21841 tagggtctgc taaatcaagt gtttctggag attttattat atccatatta gcatcccata
    21901 gactttgtat agttcctaca tctctccagt atcctttaaa tggataagca tacattccaa
    21961 cattatcttc taacatttta ggtattaaat tttttccaaa atcatcataa tttatttctg
    22021 gatttttttc tgcttcctta aaataatttc tcaacatttt ccaatcaaat atgtatactc
    22081 ccatagatgc caaattgctc ttaggttcac ttggtttttc ttcaaattca tagatacttg
    22141 aatcttcatt tgtattcatt atcccaaatc ttgaagcttc atcccaagga acttcaatca
    22201 ctgctattgt agctttagag cctttttcct tatgaaattt taacattttg ctataatcca
    22261 tcttgtatat atggtcacca gataatatca atacatattc tggattatag gtatctacaa
    22321 agtccatatt ttgatatatc gcatgtgctg taccattata ccaatttcct tctttttcat
    22381 tcataaatgg ttgtaaaaca tagacaccac catttattct gtccaaatcc caatgacttc
    22441 ccatgcctat atgtgaattt aatatcaacg gtctatattg agttaaaaca cctacagtat
    22501 caataccaga gtttgaacaa ttgcttaaca caaaatctat aattctatac ttacctccaa
    22561 atgatacagc tggtttggca attctttttg taaaaacacc taatcttgaa ccttgccctc
    22621 ctgccaaaat catagctaac atctcttttt tcatttaatg acactcccct catctagtaa
    22681 ttaatattta taattgcatt tttactttta taaactttat cagtcaataa atgtcgtgta
    22741 taaatgttgc ttaaataata ccttaataaa aataaaataa tatcaactat acaattgata
    22801 taatgtatac cccataatgg acttaccgac gtatacatta ttttccataa gc
//
LOCUS       DAEMIK010000057        22783 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEMIK010000057 DAEMIK010000000
VERSION     DAEMIK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.057"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(426..566)
                     /locus_tag="KPK87_001848"
     CDS             complement(426..566)
                     /locus_tag="KPK87_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538000.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(826..1986)
                     /locus_tag="KPK87_001849"
     CDS             complement(826..1986)
                     /locus_tag="KPK87_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG2538001.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(2157..2360)
                     /locus_tag="KPK87_001850"
     CDS             complement(2157..2360)
                     /locus_tag="KPK87_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG2538002.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2447..3481)
                     /locus_tag="KPK87_001851"
     CDS             complement(2447..3481)
                     /locus_tag="KPK87_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG2538003.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(4003..5364)
                     /gene="rlmD"
                     /locus_tag="KPK87_001852"
     CDS             complement(4003..5364)
                     /gene="rlmD"
                     /locus_tag="KPK87_001852"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG2538004.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(5391..6113)
                     /locus_tag="KPK87_001853"
     CDS             complement(5391..6113)
                     /locus_tag="KPK87_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG2538005.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYIEYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(6113..6442)
                     /locus_tag="KPK87_001854"
     CDS             complement(6113..6442)
                     /locus_tag="KPK87_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG2538006.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIREGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6772..7698
                     /gene="fba"
                     /locus_tag="KPK87_001855"
     CDS             6772..7698
                     /gene="fba"
                     /locus_tag="KPK87_001855"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG2538007.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVEGKIDDVLGSANSIN"
     gene            complement(7966..9597)
                     /locus_tag="KPK87_001856"
     CDS             complement(7966..9597)
                     /locus_tag="KPK87_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2538008.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10080..11117)
                     /locus_tag="KPK87_001857"
     CDS             complement(10080..11117)
                     /locus_tag="KPK87_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2538009.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(11136..11921)
                     /locus_tag="KPK87_001858"
     CDS             complement(11136..11921)
                     /locus_tag="KPK87_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2538010.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(11954..13087)
                     /locus_tag="KPK87_001859"
     CDS             complement(11954..13087)
                     /locus_tag="KPK87_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG2538011.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(13135..14262)
                     /gene="hadC"
                     /locus_tag="KPK87_001860"
     CDS             complement(13135..14262)
                     /gene="hadC"
                     /locus_tag="KPK87_001860"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG2538012.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(14262..15488)
                     /gene="hadB"
                     /locus_tag="KPK87_001861"
     CDS             complement(14262..15488)
                     /gene="hadB"
                     /locus_tag="KPK87_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG2538013.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(15503..16303)
                     /gene="hadI"
                     /locus_tag="KPK87_001862"
     CDS             complement(15503..16303)
                     /gene="hadI"
                     /locus_tag="KPK87_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG2538014.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(16342..17541)
                     /gene="hadA"
                     /locus_tag="KPK87_001863"
     CDS             complement(16342..17541)
                     /gene="hadA"
                     /locus_tag="KPK87_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG2538015.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            18218..19216
                     /locus_tag="KPK87_001864"
     CDS             18218..19216
                     /locus_tag="KPK87_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG2538016.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            19578..20105
                     /locus_tag="KPK87_001865"
     CDS             19578..20105
                     /locus_tag="KPK87_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG2538017.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLATTILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMSLDKLDFKKRYFN"
     gene            20132..20728
                     /locus_tag="KPK87_001866"
     CDS             20132..20728
                     /locus_tag="KPK87_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG2538018.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(20793..22375)
                     /gene="asnB"
                     /locus_tag="KPK87_001867"
                     /pseudo
     CDS             complement(20793..22375)
                     /gene="asnB"
                     /locus_tag="KPK87_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
     gene            complement(22440..22577)
                     /locus_tag="KPK87_001868"
     CDS             complement(22440..22577)
                     /locus_tag="KPK87_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538019.1"
                     /translation="MKNKKFNVITLIILDNHIYAINKEQISTNSKRIYFEKSRFFSDY
                     K"
BASE COUNT         7112 a         4094 c         2684 g         8893 t
ORIGIN      
        1 tttttataat aaattgtata cattataaat tatattaaat ttttaattta agtaatagta
       61 tatgtaatct ttagtcatct tcttaccagt tttttatcca cttttctaat gaagttattt
      121 tttcatatgt taattcctct tgaaataatg atactaaaag actttttata aaattataac
      181 ttaaaagttt atgtattttt ttgatatgaa tttatagtac ttatcttctt taattgagat
      241 tgtgtatatt taacttaaat attacatcaa taattccatg caatcaaatc aattatttac
      301 atattttttt agaacaataa taagtatata ttcacaaaat tttgtgttaa tatttttagt
      361 ttgtgatata ataaaagcaa ggaaacatat tttacttaac tttgagtgat gtttccttca
      421 acaaattatt ttgaagattt acttttctgg aactttatat taagttccca gccactctta
      481 gttggtcgct cagagtggct ctttaattta tcgtaaatgt aactggctat aacaccagtt
      541 ataatactca tcattagatt ttccatagtt ttcacctcct ctcataagaa agtgaagaaa
      601 cttccatgga aacaccactc atagcttttt caaatattta ttccttgcca tattaattat
      661 atcataaata ttggaaacat aaaattttaa tacttatcgt ctttattatt attttttata
      721 attaataatt ttctttttaa taatacataa tataaattta tatttcttca ataaaaaaga
      781 caatcttaat taattgtctt ttttaatata tatttggcta atacattatt ttacacaaac
      841 attacaaggg tctattttac cttgtgactt agcttgtttt aatgttactt tatttattgt
      901 tttacttctt tttagtgtag aacaattttt tgttagatga tatgattttc cattaggagt
      961 tacatatact gtacttttat cagttaatcc aggattatta gagctaggct cattacttgg
     1021 tttgttcgta ttatttgaat tagtatcttt tacaatacct gtatttgaat ccccaacatt
     1081 attagttata tttaaacatt gttttataac aacatcatca atatttccaa tagcagttat
     1141 tttacttgca ttttttaata tactcttatt acttccattt ccaactaaca ctataggtgt
     1201 tttttgtgca attgtagaac caactaaaga ataaactaaa tcagtcccac ttgctatgta
     1261 aaattctttt gagccattat aaaatttatt tattatttgt ttatttgtat cgtatctatc
     1321 tactccacct attcttgttg ctttagtttc atttattaac ttatcactca tactgacttt
     1381 tccaccaatt gcataagttt ctaatccatc tgtagcaaaa cttgtagtat ttccatcagt
     1441 taatactatt ggagatttat ctctaactgc tatagaagca acactcatag catctggttc
     1501 gcctttaaaa gcattagtta atattacttt tttaaccgta cttactcttc ctatttcttt
     1561 tgcaacttta tgacttgtat ctattctatc agttccactt attctattta tgcttatttt
     1621 tttatcactt aatattttct gaactgatgc tcctatcgaa ttttttcctc caattagata
     1681 tacttctttt accttttcta atctttttaa tgtttctgaa gggattgtat ctttcttagt
     1741 taacaatatt ggggcatttt tagctcctga caatccactt gctgacaatc catcagataa
     1801 tccattttca gaattaataa gtattgcaat atcataattt tgtttatctg ctattaaacc
     1861 tgctgtttca tacttatttg taccttttat tgaattaata gaactaagag cgtttgatgt
     1921 tggacaacta gcaatcaata atgaaacaga catccctatt gccaatagtg ttttgcttaa
     1981 tttcataatg aaatcctcct ataaatttta taatattact taaatattat aattctacat
     2041 aaaagaatat tttcctttat gaaattagtt tatatttcaa aattttgtca ctttttaaat
     2101 ctatatacta aaaaatagac tatttgtaac agtctatttc ttagtatatc aaatatttat
     2161 ttaggtttac gtttctttgt actagttttt gtatatgata ttccagttcc aggtatactc
     2221 aatgtagctg attttcttcc ttttgaatta actgacactc tagcaccttt aacaccagca
     2281 cttataccta cactcttctt acctaaattt aatttgagtc ctcctcctag atttatgctt
     2341 ttcctaaagt taaatcccat agatataacc ttcccttcta tattttaaat atttatactt
     2401 aaaattatat ttgttataca cttgtcatca ttggtatatc tagcatctat atctttttac
     2461 gttttttctt cttatatact actccagcaa ctacagctgc tgttccagct ccaaccccat
     2521 aagtagcatt tctacttttc tcttcctctc ttttttctaa accttcatca tatacacctt
     2581 ttaacttagt ttcaatagac ttcatttcat ctggtatact aaaatcttct tcattgtaac
     2641 cttgattata tacactttct tttgctttag gtaacttaac cttaccatta tcatatcctt
     2701 ctttaaacca attagcatat ttaacattac ctatagttga tgtgtcaaat ctttctcctt
     2761 tgatagcact ctcaaatcct tgagtagtgt aatcattttt taattgttct tgtatttcgt
     2821 cataagcatt attataagca tctataaatt cagaatctat gttttcttca aatgtcattt
     2881 tttctttatc tttattgcta tcctctatcc ctttagcttt atattcttct atctttttag
     2941 tcttatattc attgtatcca gttgaatatc cggtatcata agcatttttt aaaacttctt
     3001 tttcatatgt attattagat ttagcacctg taagccctaa attatatcca gtctctttag
     3061 ccaccttttc ttcctcttct aatttagcct tacctttttc atatccttct gagtactttg
     3121 attcatatgt atcactataa tctccactat aatttgatga actataattt cctttatacc
     3181 catcttcata cccttgatta tatccttttt ctccaacaga cctcttttgc ttttcttcat
     3241 tattaaccga agttgttgtt ccagaactgc ttcctcctcc agtataagga caaccaccat
     3301 tattatgtaa atgagctgga taaccaccac aatgatagtg ataacttcct aatccagatt
     3361 tatttttatt atcacgatga cctcctgatg aatcagttct tcctgaatga gcatacgaaa
     3421 agctccctac actaatcata gttatactca ttgcaacagc tattaacttt ttcttaaaca
     3481 taatctattt ccccctaaaa tattctatga ttttattata tcagtcaata tattatgttt
     3541 aaaccgttaa aattcgacaa tgtaaatatt tgatgaattg tatttgtaaa taaatctttt
     3601 agcatatctt gaatatctgt agttgttttg acatcatatt cttcaataag ttttttaata
     3661 tttcgttttt gttaaacata aaaagcatgc actttccttt gtatagtgat atttgatatt
     3721 ttaattatca ctactttaga agaatacaca ctttatttta aaaaccctga tactatgtta
     3781 ttagaaaatg gtactaatat aaaagatatc cagcatatac tcggacatgc taaaacttca
     3841 acaactatgg atacatattt acatgtaact aataacatga gaaaccagac ggtaaatatt
     3901 tttgaaatca ttgtagaata aatttgccac ccaaaaataa ttatggtggc aaataggtgg
     3961 caaatctact tttaagacac aatataaaag tctgaaaccc tgttattctc taataagttt
     4021 aactacagtc tcacaatgtg gcgtatttgg gaacatatcc atacacttaa ccatcttaat
     4081 ctcataacca taccccttaa aatcaaccaa atcaacaacc aaagacttag gattacaaga
     4141 aatataaaca atctccttag caccaaatcc acaaatatcc ctaatagcat ccttatgaat
     4201 gcctggtcta ggagggtcaa caataatcaa atcaggcttg tcctttaaat ccttaacaac
     4261 cttagccaca tccccagcaa taaactcaca attagtaagt ccattcaact tagcattctc
     4321 attagcagca acaacagctt cctcaactat ctcaatacca tacactttct tagcagcacc
     4381 agccataacc tgtccaatag acccagtacc actatacaag tcaaatacaa ctttatcatt
     4441 gtaatctcca ataaaatccc tagcaatact ataaagaacc tcagctccct tagtattggt
     4501 ttggaaaaac gaaaatggag aaatcttaaa cttaagacct aaaatttctt cttgtatata
     4561 atctcttcca tacaatactc ttaactcatc acattggact gcatctgcta aaccatcatt
     4621 aatagtatga agtaccccca ccaactcaga ttttaaatca atccctaaaa gcatatcttt
     4681 aaactcattc atatcaaaat cttcttgaga agaagttact atattaacca taatctcatt
     4741 agtatgaatc ccttttctaa ctacaagatg tctcaaatat cctttgtgat tcattgttct
     4801 ataataagga agcttttttt cattgaaaaa ctctacacta gaaactaata tctttgagaa
     4861 gtcttcatca accaacatac actcctcaac agtttgtata tctatatgct ttcctttttt
     4921 atgaagacct aatgtcaaag gtccattttt tacttcatct ccaaaagtat attccatctt
     4981 atttctataa tatttgtttt ctggacttcc ttctatccct aaaaactgaa atccaaataa
     5041 atcttgcttt aaaaataaat ccataacttg tttttctttt atttctaatt gtttttcata
     5101 tggcacagac aatagtgtac atcctccaca ctctctaaag tgcttgcaag tttcttcagt
     5161 ttcaagtggt gagttctcta ataattctat catcttaact tcagctttct tacttctaac
     5221 ttttttaact ccagctctca ccttttgacc acttattcca cccttcatat ggataactct
     5281 atctccaact tggcttaaag atgtgccacc aaattccatt ttatctactt caaattctat
     5341 aacatctttt ttcttcacag acattcctcc aatttactat agtatcttta ttatctttct
     5401 tcaatcaatt ttacttcttc atctgttaat tctctatact cacctaactc taaactttca
     5461 tccaatttta aattgcccat agacaatctt tttaagtata ctactttttt gcctacgctt
     5521 tcaaacattc tttttacttg gtgaaactta ccttcatgta ttgtaagctc tatttctgat
     5581 tcatcatcac tttttaatat atttaactgt gatggcatag ttttatatcc gtcatcaagc
     5641 actacacctt ctaaaaaagc ttctacatct tcttcagtaa ctactccatc aatttttgca
     5701 taataagtct taggcacatg cttcttagga gataaaactc tatgagaaag ctttccatca
     5761 ttagttaaaa ctaataatcc ctctgtgtct ttatctaacc ttccaacagg aaatggttca
     5821 aaagctaagt atgataaatc aattaaatct aatactgttg ggtcatattt atctgtagtt
     5881 gctgatatat atccatcagg tttattcatc atcaaatata tatattctat atatataact
     5941 tcttctccat taaataatat ctcatcattt tcagtgtcta cttgagttcc tggatttgaa
     6001 acttcacttc catttactac tacagaccct tgtttacaat acttctttat ttcacttcta
     6061 cttccataac ctaaatttga aagaatttta tctatacgtt gctttttagc cattaaaaat
     6121 gctctccatt ctcataatat ttttcttttt caaatactgt aaatgctaaa tccatacttt
     6181 ctacatctaa attatttctt atactatcag ttattattct tgctactata tcttgtactt
     6241 cttgtcctct atcaaaccaa gctacttcaa caaaaggata tacatctgct attttaccct
     6301 ctcttattgc aactgacttt atacattcta tttcaaagta gtctcttgga cattttacag
     6361 cttcaactaa atcatttatc attttttcac ttattttaca tatatcattt tcattaattc
     6421 ctcttatttt tatctgtggc atagtttatc tcctttattt taaattttta gtgaattatc
     6481 atccacttaa ttcccttcga taacgtttga agtggtaact tcttggtcaa gacaactatt
     6541 gttatcagta tctccaaact aataaggtaa ttcctaccct tatagttagc attatatagc
     6601 tagttttcct aaattgttta tattctttat tattttattt aatcctattg tcttctattt
     6661 aacattatac ttgtaattta tagttattac aatttactat atactttttt tgatttaacg
     6721 ttataatata agtgatgggt aaattttaaa agttaatagg aggagtttaa aatggcatta
     6781 attactacaa aagaaatgtt taaaaaagct tacgaaggtg gatttgctat aggtgcattt
     6841 aacataagtg acttagaaca attacaaggt gttttaaaag ctgctaaagc taaaaattct
     6901 tatgttatga tacaagcttc tatgtcagct gttaaatatg ctggacctca tacattagtt
     6961 gaaatggtta aggctgcttc tgatgaaata ggtgtagatg tagctcttca tttagaccat
     7021 ggtccaaata tggatgctat taaaacttgt attgatgctg gtttctcttc agttatgata
     7081 gatggttctc attttgactt tgaagaaaat gttagaataa ctaaagaagc tgttgaatat
     7141 gcacactcta aaggtgttgt tgttgaagca gaacttggtg ttctagctgg aacagaagat
     7201 gatgttactt ctgatgttca caagtatact caaccagctg aggcagttga gttcgttgaa
     7261 agaacaggtg ttgattcatt agcaatagct attggaactt ctcatggtgc tttcaaattc
     7321 aaaggagaag ctaaattaag attcgatata ctagaagaaa tccaaagcaa attaccaggt
     7381 ttcccaatag ttcttcatgg tgcatctgct gttgaccaaa acgctgttgc tacatgtaat
     7441 gaatttggtg gaaatatagc tggtgctaaa ggtgtacctg tagatatgtt aagaaaagca
     7501 tcttctatgg ctgtttgtaa aataaacatg gatactgact taagactagc tatgactgct
     7561 gctatcagaa aattcttagc tgaaaatcca aaagaatttg acccaagaaa atacattggt
     7621 gctggtagag atgctataca agctgtagtt gaaggcaaaa tagatgatgt tttaggttct
     7681 gctaactcta taaactaata taacttttaa atatttataa aagaaaagag atgacttgaa
     7741 ataattaaat ttatttttaa gccatctctt ttaagttgta tcaattatgt atctttataa
     7801 cattctaatt aaattttaaa aaacatgtat atttatagta tgtttataat agttatgatg
     7861 tgttatatcc aattattatt ttaaagaaca tatatattta caatatactt aaaatctaca
     7921 aaatatatta aaaattttaa ttatattgtc tttttaaact ggcaactaaa catgaatgtg
     7981 tccacttttt atctttctat gaatagtaga aggatatata cctataactt cagcggcctt
     8041 tgttattgaa ccatacttat ttatagcctt acttatcatt atttggtcca ctattttata
     8101 tgcatcatta agattcataa ttccatttac cactattggt gataaatcat ctgtttcatt
     8161 atcaatcata tctaaattta tcagcatgtt aaattcatct tctccaatta catttttagc
     8221 tgataatact acaaatcttt caatgatgtt ttttaattct cttatgtttc ctggccaaga
     8281 atattcttct aataactcaa taactttagg cgaaacttct acttctctat tgtacttttc
     8341 attgtaaagt tttaagaaat aatttactaa tggtactata tcctcttttc tttctctaag
     8401 aggaggtatt tttactggaa taacacttaa tctatagtac aaatcttgtc taaatttaag
     8461 gttatttcta aggtcttcat gagaaatatt agtagcactt atatatctaa catctatttt
     8521 tataggctta ctaccaccta ttctagtaat agtattttct tgtataaccc taagtaattt
     8581 tttttgcatc tgcattggta actcacctat ctcatccaag aaaatagttc caccattcgc
     8641 ctcttcaaaa agtccctttt tacctttttt cttagctcca gtaaaagaac cttcttcata
     8701 tccaaaaaac tctgactcta ataattcatt tggtatagct ccacagttta tagctataaa
     8761 aggtttgtct ctccttgaac tatacttata tattgttctt gctattattt cttttcctac
     8821 acctgattct ccatttataa atatagaaga atctgaggct gcagctctct taacaacttt
     8881 ttttatattg tccataatct tactttgact tatatactct gtgtcttctg atatatcact
     8941 atattttatc tgagattcaa gcttaataac ccttgaaata tctctccctg ttagtactcc
     9001 tcctgtaagc tttcccttag aatcccatct tggaactgcc gtatatgcaa taagcttccc
     9061 atctgggtaa acgatatttc tataaatctt tttcttcact ttaagcatgt ttaatattat
     9121 tggttcttca actaatcctt gtctcacaag gtctgttata tttctaccaa gtacctcagt
     9181 tcttttaaaa ggtaatatct catcactgac agtgttcatc ttttcaatta ccccatcttt
     9241 gttaaacaca acaacaactt catctatatg ctctattata tcttcaatta ctttatcaga
     9301 tattagagac ttctcaaata aataaactat attattttca ttattattat ctttcatatt
     9361 gtatatatgt gcagtcatcc ttaactctct gaaagctatt ttctttttac tatctgtcag
     9421 tatttctgtt ttgtcaaaat ttgaatctaa ttcatttata tttctaggct tttttatatc
     9481 tagaaaacta aaaatactat ctattagatg attacagtat attatttcgc ctgattcttt
     9541 acataaaatg gctggatttt ctacatgttc tataagccct attaaattat tatccattaa
     9601 tattcgcacc tttcattaag taatttattg aactacatac aaaatttatt gtttgcttac
     9661 aataaatacc atcagcacat ataatcaact ccttataata ttcgtctttt caagtagttt
     9721 atttcattaa aacgtaacta agatataatt ttaatgatta tagtttgtta aaaatttcat
     9781 tttgctttta attgtatttt atcacatatt tagtaattat ttgtctattt aatacaattt
     9841 aattaaattt aagatttatt taaaaaaaca aagataatcc ttacagaata taactcacaa
     9901 tttgtaagca atatacatat aaggattatc cagattatct tgtattataa aattttattc
     9961 aaacagactt tttaataact gacaaataca cactagcaat aacttatcaa agggttaata
    10021 attatgttac taactaataa gacttattca atcagtaata tttatatagt ttgtatctat
    10081 taagctttta tagcttctaa taaatcatca acgctatcta atttttctat aagcattgga
    10141 actatttcat gtaagtctcc aactactcca tagtcagcga tttctaaaat tggagctgct
    10201 gggtttttgt ttatagcaat tatgaaatct gatgattgca tacctgctaa atgttgtatt
    10261 gctcctgata taccacaagc tatatatagg tttggtttaa cagtagttcc tgtttgacct
    10321 acttggtgag aatggtctat ccatccagca tcaacagccg cacgagaaga accaactact
    10381 ccacctaatt tgtcagctaa ttttttaagc atttcaaatc cttctggtcc acctaatcct
    10441 aaaccacctg atactataac atttgcatct gtaagagata ctaaatcttt cttagtttta
    10501 actgtttcta aaacagtagt tcttatatca tcttgagtta ttttgtagtc taatgctata
    10561 acttcaccag ttcttgtttc gtctttttca gctttatcca taacaccagg tctaactgta
    10621 gacatttgag gtctatggtt tggacaaatg attgtagcca ttatgtttcc accaaatgct
    10681 ggacgagttt gttttatttt cttatcttct gggtctattt ctaacttagt acagtcagct
    10741 gttaatccag ttccaactct tgaagctatt ctaggtgcta agtctctacc tatatgagtt
    10801 gctccgaaaa gcattatttc tggttttatt tcatctattg catcttttat tacttttgta
    10861 tatgcatcag ttgtatattt ttctaaaaga gcatcatctg caacataaac tttgtcagct
    10921 ccaaaagtga ttaattcttt agctaatcca tcaacatctt ttcctaataa tattgcacaa
    10981 agttctgcat ctacttcttt agctaatttt cttccttctc ctaataattc tataactact
    11041 ggagctattt ttccttctct ttgttctgca aatatccata cgtttttata agaacttaaa
    11101 tcttttatat cattcatagt ttgtactcct ccaacttata taatgtgctt ttgttttaga
    11161 ccgattatta ttttactagc tatttcatca gctttacctt ctaagatttc tccttttcct
    11221 cttggttcag gagtaaatga tctaaatact ttagttggtg atgcttttaa accaacttct
    11281 tctttattta catctaaatc atcaattgtc caaactttta catcatgttt atatgctttt
    11341 actattttat caacagacat gtatctaggt gtatttaact cttttactgc tgttaaaagt
    11401 acaggtgtac ttacttttat taattcgtat ccatcttcta attgtctttg aactattata
    11461 tcttttcctt ctattttgaa atcttgtaca tatgtaactt gaggaatgtc taatttctct
    11521 gcaatttgtg gtccaacttg tgcagtatct ccatctattg cttgtcttcc tgcaaatatt
    11581 atgtcatagt cacctacttt agatattcct gctgcaatag tatttgatgt agcccaagta
    11641 tcagctcctc caaatgctct atcacttaat aatattgcat catcagcacc catagctaaa
    11701 cactctctta gcataaagtc tgcttgagga ggtcccattg ttataactgt tatagttaca
    11761 tcatcatgct catctttaat ttttaaagct tcctctaatg catttgcatc atctgggttt
    11821 aatatacttg gaactccatc tcttataaga gtacctgttt ctttatttat tcttacttcg
    11881 ttagtatctg gaacttgttt tacgcaaact aatattttca aagtctatac ctccaatttc
    11941 attagattat ttattatttt aatacgtttg atgaaattac cattttttga acttctgatg
    12001 ttccttcgta tattgatacg attctagcat ctctgtacat tctttcgatt tcataatctt
    12061 ttataaatcc atatccaccg tgaagttgta atgctttata agcaacttca tttgcaattt
    12121 ctgatgcata gtatttagcc atagcagatt cttgagcaac tctcattcct gcatctctct
    12181 tttgtgctgc atcataaact aatcctctag ctgcacaaac ttttgtttcc atgtcagcta
    12241 tagtaaattg agtattttgg aatttagcta taggcttacc aaattgaact ctttgttttg
    12301 tatatttaac agcttcatct aaagcacctt gagctattcc tagagctaag gcagcaacac
    12361 ctattctacc aacttctaga gtacccattg ctatcttaaa tccttgacct tcttttccaa
    12421 gtaagttttc ttttggaact ttaacgtttt caaatattaa gtcagaagtt tcagttcctc
    12481 ttatacccat cttatcttca tgagctcctg ttgagaaacc atcccattta ctttctacta
    12541 taaacgctga tgttcctcta agtcctttac ttctatctgt aactgctatt actatagcta
    12601 catcacagaa tggtccatta gttatgaaag tttttctacc atttaatata tagtggtctc
    12661 caactaattc tgctgttgtt tgttgtccac ctgcatcaga accagctcct ggctcagtta
    12721 aaccaaatgc tcctattaat tcacctgttg ctataccttt taagtatttt tgtttttgct
    12781 cttcagtacc attgtaaagt attggtaatg aagcaagaga agaaccagct gtaacaaaag
    12841 tagctgttga agcacatctt ctagctattt cttccattac tatagcgtaa gaaacatatc
    12901 ctgctccccc accaccatat tcttctggga tattactgct tataaatttt gcatcagcta
    12961 attttttgat taattcctta ggcattacac ctgttttatc catttctgct ggtaaagtat
    13021 ctaattcttt actaacaaaa tctcttactg cttttcttaa aagttcttgt tctttgttat
    13081 ataacatagc ttcctccaat tttcatattt tatagtaaat ttattttaat gaacttacgc
    13141 taaactcatc atctcagcaa aagtttgaat acgagttctg gcttgttcat tattctgagt
    13201 ttgttggtcg atttcaacat ataaagtagg tattccacta tcttctatat cttttctaac
    13261 taaaggataa tcgtattctt ctgggtcaca gaatttcatc atacagaaga taacaccatc
    13321 tatatctttc tttttaactt catctactat aagtgaccca cgttttttct tagggtcata
    13381 agctaatgaa catccttcta tgtttgacca ttgtcttgct aatctttcta acgcatcatc
    13441 acctgctggt acatctgttc tgaattgtct tgtttcttgt gctaaatcgt cagctacaac
    13501 tgatatattg ttgtcttcta aaatgtctaa tatatcttta gaatcagcta atatacctgt
    13561 taataaaact ttctttccag aacagacttc ttctggcata gcatttaatt ttgctattaa
    13621 atctttcact agctcagtat gttcttcttt tctcataaag aacccactct taattactaa
    13681 gcttcttatt gatggtttta tagtattaga atgtttataa gctacttcaa caaagtctct
    13741 catagttttt ctatgttcat tgtaaacttc tatactttca tgaatttttg cttcttctat
    13801 ttcatatcca caaatttctt caagttctct ttttacgcct ttgtactcac tgattaagta
    13861 ttttacacca gcttcaagtt ttctattttg tgggtgtact aatgatatat attttatatg
    13921 aggtactgct gatttccagt tttgtcctaa acaaattagt gtatcacaca tacctggtat
    13981 aataactcca gataattcat cataagcacc ttttaatcca tattctaaac atgattgcat
    14041 tattgaacat gcaaatgcag ggaaatattg tttagctaaa tctaattctg tttgtcctcc
    14101 ccatatacca actggaagca ttccagctgc atgtataatt tcttctgggc aataaactgg
    14161 gaaacaacct atagcttttt taccagtttc acttttatat ttttttacag ccgcatttgg
    14221 attttcaact acttctttca ttttagataa aatagcttcc attatatctc acctctatta
    14281 agtttttttc tttcttccat tacttcaact aaaccttgaa ttcttgtttc aaattgagca
    14341 ttagtgaaag ctctagggtc tgcttggtca ccatcaaatc cagcatatgg taatccagtt
    14401 tcttcagctg ctcttctttg catttcatat tgtattaaac tcataagttt acagcttctg
    14461 ttcatatgat agaatgctcc atcacattta ccctctacta aagaatcaac tctatatttt
    14521 gtcatacggt ctaggtttac attgttaaac atagtactat atgctactgc cattccatct
    14581 aaatcattaa cttcatattg taatgcccaa gcatgtgggt aaacactacc tgtcatgtta
    14641 actccaaatt tagctaatgt tttcatcttg tatcctatat atggccaaca aggtatacct
    14701 tccatcatta ttctgtattt ttcttcccct ctgaaagaag atttaccagt tttcatgttg
    14761 tcctctaatt cttctataag taacttaaat gcttctgtag tttctttttt acctctagca
    14821 caaactatta cagccatgta agtaaataag tcaaatccat tcattggaga aggagaagaa
    14881 tctgctggta aactcataga atacttccat aatcttccgt tttcagctga tattttcatt
    14941 acttcttcaa acttcttagg gtcaaatttc tttcctgata taatttctag ttgttttata
    15001 gcttcttcaa attgagcttt aatataatct attcttgatt gagtaacttc gtcttcatta
    15061 ttgaaagttg tatcaatcat tattaaaggt atatctaatt ctcttgaaat attttcatac
    15121 cattttataa cttggttaca tatattgtta cagcaaagta ggaaatctgg agctggcata
    15181 tccaaagctt cacatccacc attttctaaa agaccaaaat ttgttcttgc atatgcacat
    15241 aggtcaatag aatatccttt agattcagct atttcacata attctaaaga acctttttta
    15301 gctgctactc cagctgcttg gttttctgga tataatacat ttaagtcaaa tacttctgct
    15361 aactcttgag gaaatactga tgttgaccaa cctacaggtc ttccttcttc tttagcttta
    15421 aatgcatttg catattgttc agctaataaa tcattaatta ctactctagc ttcttttttt
    15481 tcagacattt tcatccctct ttttatatat ttttcacttc tttttgtgat tcttttgctt
    15541 catcaaaagc atataacgct gctcctaaag caccagttaa ttgaggtata tcaggtacaa
    15601 taatttctgt gttgatttct cttgccatag ctcttacaat accactattt ctagcaactc
    15661 caccaaccat aactacattt ctttgtactc ctattctttt tactaggcta gaaactctct
    15721 ttgctactga agtatgaata cctgccacta tatcttcaat ttttgcattt tcagataaat
    15781 gtgatataac ctcagactct gcaaatactg tacatgtact gcttattgat acttcatttt
    15841 gagaattcat agatatactt ccgagttcag atacatcaac ctctattatt tttgccatta
    15901 catctaaaaa tcttcctgta cctgcagcac atttgtcatt cataagaaag tttaatagtc
    15961 ttccattatt atctaatttt aatacctttg catcttgacc acctatgtca ataatggttc
    16021 ttgtctctgg aattatgaaa tttacccctc tagcatgaca gcttaattca cttatttgct
    16081 tatcagcatc tgaatagttc attcttccat atcctgtaac tacaactttt ttaatatctt
    16141 ctcttgcaag acctgtcttg ccatataatt tttctaaaac tcttgatggt ccagtagtcc
    16201 cagtaccaga ggatattgtt tcagaagcta caatatcttc cccattcttt aagattactc
    16261 cctttgatgc agttgaacct atatctaatc ccattgtgta catctttagc attctccttt
    16321 taaaaaatct agaattacta attaatatct tacaacttta ctatctttaa agttatttat
    16381 tttctcttca tcgtagccta aagattttaa tacttctaca gtatgttgac ctacttttgg
    16441 tgctggtgta tattctttaa ttccttcatt tctaaacata actggagtat taactaagac
    16501 acctgtattt ccgctatcgt atgttttctt aaataagaag tcatttgccc aagcttgttc
    16561 gtcatctaat aaatcttcac agctttgaat tttttcaaat ggtaagtctg cttcttctaa
    16621 taaagctgac cactcgtcta atgttttttc taacatagct tctccaacta tcttaactaa
    16681 atcttcaaca tgattaacca ttgaatctat gttattatat ctatcatctt ctaatatata
    16741 ttctctattt ataaccttac agaatttgcc taaccacttg ttgtattgta ttaaagctaa
    16801 ttgaatccat cttccatctt tacatttata tgtagtcatt aatgggctgt ttggattttc
    16861 tcttgataaa ggcatttcat ttccgtattg tgctgttgtt atcattgttc ccattccata
    16921 tatagctgta tggaaaagac ttactgttac tctctcacct ttaccagttt gagctttttt
    16981 atgtaatgct gctaaacttc ctgctgctag tgctagacct gcatagtggt caccaaatcc
    17041 tgctgctgta tttgctggag atgttccttt ttccataaca gattggctaa ctcctcctct
    17101 tgcgaagtat gcagtatagt caaatcctgg tttatctttt aaaggtcctt tttcaccata
    17161 tcctaatatt tgagagaata ttaatcctgg atacttatct tttatttggt cataagctat
    17221 acccattttt tctaatgctt gaactctaac attagttaca aatatgtctg cttctgataa
    17281 taatttatgt aatatttcta ctccttcttt tgattttaca ttaatactta cacccttttt
    17341 atttccattt tctaattcaa acatagggtt ttcatcatct gatgctggag atttaaatgt
    17401 tccacccata actcttattc catcaccttc tataggttca atcttaataa cctctgcacc
    17461 ccagtcacct aacatttttg cacaacatgg tgctgcgatg aaacttgaaa gttctactac
    17521 tttaactcct tctaaaagca taacgttttc ctccttaata gtatcaatta taaaatttcg
    17581 attaaatcac tcataaaata cttttttctg atattaactg tctgatgtca aagttcctaa
    17641 tcttatctaa aataacttaa aaattattaa aattattttt tattgtttta ttgtcttaca
    17701 cctatattgt taagcattta tcgtgccaac tttatattta atcaaaatat cgcttgattt
    17761 gaatgttttt taacaatatg caattgaata atctttttca tatcgtggca tcttgccact
    17821 atccattgca ttttgctatt ctttctatgc ttataaaagt tttttttata tattttttta
    17881 tgaaaaagct tgacatttca aaaaaagttt tcattgctaa actcaaaaac acatgttatc
    17941 attgtaatta atcagcaagt tagttaacta atttcaaact tagataattt tttaataata
    18001 tttaatctaa aaaataacaa tttctttgat aaattttaga gtaattatta cattaaataa
    18061 aaaattacta aatatcaaaa taacacaggg aaaacaattt caaaactaaa tttttaacct
    18121 taaaaatttt gtataaaaaa tataacctta tatgctacat attatttcta aatgaaaaat
    18181 taccataata atttaataat tacaggaggg tattgatatg aaaatactag tatttggagc
    18241 acgcgattat gaagaaccag taataaaaaa atggtctgaa gaacataagg atgttcaagt
    18301 ggatatttat cctgaaaaca tgactgaaga aaatgtagtt aaagctaaag ggtatgatgg
    18361 tatatctata caacaaacta actatataga taatccttat atttatgaaa ctttaaaaga
    18421 tgctggggtt aaagttatag cttcaagaac tgcaggggtt gacatgatac attttgattt
    18481 agttaatgaa aatggactta tcgttacaaa cgttccttct tattcaccta atgcaatagc
    18541 tgaattagct gttactcaag ctatgaacct tttaagaaag actcctctag taaagaaaaa
    18601 agtctgtgaa ggtgattacc gttggatagc tgaacttctt ggaacagaag ttagatctat
    18661 tacagttggt gttataggta caggaaaaat aggtgctact tctgcaaaat tattcaaagg
    18721 cctaggagct aatgtaattg catttgacca atatccaaat agtgatttaa atgatatatt
    18781 aacttacaaa gattctttag aagaccttct aaaagaagct gaccttataa cattacatac
    18841 tcctttactt gaaggaacaa aacatatgat aaataaagat actctagcta taatgaagga
    18901 tggagcttac atagtaaata ctggccgtgg tggtttaatt aatacagggg atttaataga
    18961 agcactagag tcaggaaaaa ttagagctgc tgcccttgat acatttgaaa ctgaaggatt
    19021 gttcttaaac aaaaaaatga atcctggaga attaactgac ccagaaataa ataaacttct
    19081 ttctatggaa caagttatat tcactcatca ccttggtttc ttcactagta cagcgattga
    19141 aaatatagtt tattctagtt taagtagtgc tgtagaagtt ataaaaacag gaactgctac
    19201 taatagagta aattagtatt ttataaatat attttttcat taattagcta ttatctaaca
    19261 aaatagcctt taatttccca aatataagag agtcagctca gtatatattg agtagactct
    19321 cttatattat tttttatatt aaacttgtac tcttcttatt cataccaaat taaatttata
    19381 ttaacttatt ctaactactc ctactctata cattttacct atttttactt ttctaattat
    19441 atacatttga atgggtacaa tctgtatttt acaaaaaaaa cacctaacaa actatgaaaa
    19501 ttgtatgatt aatattaata tatttataag aaaacataac aattatgtta gctctggatt
    19561 tttaaggagg gagtatattg gaaaaaatga ataaagttac ttctaaaaaa actacctata
    19621 ttgttacttc agcattattt gcatccataa tatgcctgac aatagcatat atattacata
    19681 ttccagttgg tggaaacaat ggatatgttc atataggaga tgcttttatt tatctagcta
    19741 caactatttt gccaacaaac tatgctatag ctgcttctgc aattggtgct ggtctagctg
    19801 atttatctac aggtgctgct atctgggtaa taccaactat aatcataaaa ccaatacttg
    19861 tattattttt cacttctaaa agtgacaaga taataaataa gagaaatata gttgcttcgg
    19921 ttgtagcagg tatagtagga ttagttttat acatgtttgc tgaaggtata attattggaa
    19981 gttttacaag tgcatttgta atgagcttac ttggcttatt acagccaata ggaagtttta
    20041 ttgtttttat aatcttaggt atgtcacttg ataaacttga ttttaaaaaa cgttatttta
    20101 attaagaatt taaatttatg gagggaaaat tatgaatatc ttatatacaa ttgaaaatag
    20161 tatctatgta aatataacta atacatgtcc atgtagttgt gttttttgta taagaaacga
    20221 aaaagatgaa gttgcaaata gtggtagttt atggttggag catgagccaa gtgtagatga
    20281 agtaaaagaa gccttcaata aatacaattt agacgactat gatgaaatcg tattttgtgg
    20341 atatggcgaa ccattaatga gaataaatga attgatagag gtagcaaagt ttataaaaga
    20401 aaaaagtagc ataaagataa gaataaatac aaatggtcta agtgatttaa tacataacaa
    20461 aaaaacagct attctgttaa aagatgttat agatgcagta tcaataagtc ttaatgcccc
    20521 aaataaagaa gcttataata gagttactca accaaagttt ggagaaaaat catttgatta
    20581 tatgttagat tttgcaagag attgcaaaaa atatataaaa gaagtagctt tttcagtagt
    20641 agatgaaata tctccagaag aaatagaaga atctaaacaa ctggcaaaaa aacttgatat
    20701 accattaaga gttagacata agaattagca agtaaaatta taaaacatta agaatcacct
    20761 gaatggtatt tcaggtgatt cttcctcttg tattaacatt ttacaaaata gatttcatac
    20821 cacactagga atgtgtaaat catccaaatc tttctccaat tatcagagtt tccactaatg
    20881 tagtcattaa aaatctcatt aatctcatct atgttaaaga atttatctgc tatctcacta
    20941 ttgaatttat ctcggatatc cttattatat tggtcatcag ccatccatat acgaattggc
    21001 acaataaatc ctaatttatt tctaaaagca atctcttctg gtaatgtttt gatgcagctg
    21061 tacgaaaggc aattttattc tcttttcctt tcactttata ttctgatggt attctggatg
    21121 caatatcaaa tattcttgta tctgtaagag gcattctgac ttccaatcca actgcagtac
    21181 tcattttatc tatattaaaa taaatatctc cttccaacca tatttgaata tctacatttg
    21241 acatgttagt aagtgtatct agttcttctg tttcttcata aattcctttt accacttcaa
    21301 taggaagcac atcaggattg tatttcttaa gaatttttct tttctcaccc tcattcataa
    21361 tgtttgtatt tccaatataa aatgtcttca aattctctcc ataacactct gccttatggt
    21421 atatattata tccaccaaaa aactcatctg ctccttctcc agagtaacag atatttgtgt
    21481 tttcagctgc tgccttgcat ccaatagcaa aaacgatagc agatgcatct ccagttggtt
    21541 gctccatatg gtacattaca taaggtactg acgcaaaata ctcttcaggt gtaattatat
    21601 atctcgaatt tttacaccca agaatattag ctgtctgttg tgctaatttt gattcatcaa
    21661 aacgctcttc ctcataacca catgaacctg ttttttctat atctgacatt gccaatatat
    21721 aagatgaatc aacacctcct gataggaata actctgcagt ttcattctct gattttacct
    21781 ctggcataat tttctgaagt gttgtatgaa tttcatccgt ccattcctca atagacttgc
    21841 tgtggtcagg atgaaattca ggtttccaat aacgaccaat acatactgtt ttattctgat
    21901 agacaaggta atgtcctggc ataagcttct ttactccacg gaagaatgta ttttcacctg
    21961 ctacatatgt gaaagacata taaatctgta acatgtcttc atttaactct ttcacaaatc
    22021 ctggttgctc cataatctta cgaatagatg aaccatagag aagcttccca ttttcagtct
    22081 cataataata gaaaggtttt actccaaact ggtctcttag gcagaagagt ttcttttcca
    22141 cagtatccca taatgcaaag gcaaacattc catgcatgtg atttgccata tcatatcccc
    22201 atgtcttata tgcttccact aaaattgctc gttcacgctc atctctacat aatgaagaat
    22261 cgattccaag ttcttcacag agaactttcc agtttcgaat atgtccattg tattctagaa
    22321 tttttatcat atatgtgtct tttcgtgtta aatacttttc ttctaaattt atcatatgtg
    22381 tatcctttcg tattaaaaca tttaacttct aaatttatta aatgtttata tatatttatt
    22441 cacttataat cactgaaaaa tcgacttttt tcaaagtaaa ttctttttga attagtacta
    22501 atctgctctt tattaatggc ataaatatga ttatcaagta ttatcagtgt tataacattg
    22561 aattttttat tcttcataaa tattgccccc tttatattac caactaaata taatatatct
    22621 tatgttactt acatattctt taagaaattc tgaagatttt ataaagtttt ttgtattata
    22681 attaattaaa aataaaattc tagttataaa acatattttc cattaaacaa aaaaatagtt
    22741 aaaataaatt cataaccaca taaacttatt ttaactattt gta
//
LOCUS       DAEMIK010000058        22593 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEMIK010000058 DAEMIK010000000
VERSION     DAEMIK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.058"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..640
                     /locus_tag="KPK87_001869"
     CDS             <1..640
                     /locus_tag="KPK87_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2538020.1"
                     /translation="KVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEVISDLD
                     SVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKNSKLIG
                     VRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVFEYTNL
                     KKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            781..2052
                     /gene="brnQ"
                     /locus_tag="KPK87_001870"
     CDS             781..2052
                     /gene="brnQ"
                     /locus_tag="KPK87_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2538021.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2254..3441
                     /locus_tag="KPK87_001871"
     CDS             2254..3441
                     /locus_tag="KPK87_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG2538022.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3671..5695)
                     /locus_tag="KPK87_001872"
     CDS             complement(3671..5695)
                     /locus_tag="KPK87_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2538023.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            6085..6753
                     /locus_tag="KPK87_001873"
     CDS             6085..6753
                     /locus_tag="KPK87_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538024.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            6755..7240
                     /locus_tag="KPK87_001874"
     CDS             6755..7240
                     /locus_tag="KPK87_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG2538025.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            7301..8422
                     /locus_tag="KPK87_001875"
     CDS             7301..8422
                     /locus_tag="KPK87_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG2538026.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            8703..9893
                     /locus_tag="KPK87_001876"
     CDS             8703..9893
                     /locus_tag="KPK87_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2538027.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    10027..10282
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10341..11342
                     /locus_tag="KPK87_001877"
     CDS             10341..11342
                     /locus_tag="KPK87_001877"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG2538028.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            11446..11835
                     /locus_tag="KPK87_001878"
     CDS             11446..11835
                     /locus_tag="KPK87_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG2538029.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            11996..13204
                     /locus_tag="KPK87_001879"
     CDS             11996..13204
                     /locus_tag="KPK87_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG2538030.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(13290..14366)
                     /locus_tag="KPK87_001880"
     CDS             complement(13290..14366)
                     /locus_tag="KPK87_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2538031.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            14595..15122
                     /gene="hpt"
                     /locus_tag="KPK87_001881"
     CDS             14595..15122
                     /gene="hpt"
                     /locus_tag="KPK87_001881"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG2538032.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            15211..16143
                     /locus_tag="KPK87_001882"
     CDS             15211..16143
                     /locus_tag="KPK87_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG2538033.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIAAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            16205..17431
                     /locus_tag="KPK87_001883"
     CDS             16205..17431
                     /locus_tag="KPK87_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2538034.1"
                     /translation="MKKIVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            17515..17736
                     /locus_tag="KPK87_001884"
     CDS             17515..17736
                     /locus_tag="KPK87_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG2538035.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            17925..18095
                     /locus_tag="KPK87_001885"
     CDS             17925..18095
                     /locus_tag="KPK87_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538036.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(18188..18649)
                     /locus_tag="KPK87_001886"
     CDS             complement(18188..18649)
                     /locus_tag="KPK87_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538037.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            18832..19299
                     /locus_tag="KPK87_001887"
     CDS             18832..19299
                     /locus_tag="KPK87_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538038.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            19342..21105
                     /locus_tag="KPK87_001888"
     CDS             19342..21105
                     /locus_tag="KPK87_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG2538039.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            21113..21721
                     /locus_tag="KPK87_001889"
     CDS             21113..21721
                     /locus_tag="KPK87_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2538040.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            21793..22362
                     /locus_tag="KPK87_001890"
     CDS             21793..22362
                     /locus_tag="KPK87_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG2538041.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         8602 a         2397 c         3862 g         7732 t
ORIGIN      
        1 taaagtgcaa aaacctaaat ttacaggaga attagaaatt attgaaaatc ttgaagatat
       61 acttgaagat atatttaatg atgaaaaaat atttaataaa attatagaaa atgaagttat
      121 aagtgattta gattctgtaa gagttagctt tgaatcttgt atatttagaa atgtgtcttt
      181 tgaaaattgt aatttaaaaa agattgattt acttgatgtg gtatttgaaa actgcaattt
      241 atcaaataca ttttttgatg gaggaagtat atacagagtt gaatttaaaa atagtaaatt
      301 gataggagtt agatttgatg attgtatact taaaaacgtt ttgttcaagg atgttttagg
      361 taagtattct aacttttcat tctctaaatt taagatagtt tctatcttag attcaaattt
      421 tgaaaattct gtatttcaag aagtaaaagg tgacaatcta gtatttgagt atacaaattt
      481 aaaaaaagca aactttaatg gaactaaact ttctggcata gattttacta ctaataatat
      541 tgagggaatt gagattggta tagatgatat tagaggagca atttttgatg tgtcacaagc
      601 aattgattta acgaaattga tgggaattat cataaaatga aaaaaatatt cataaaataa
      661 attgggaaaa cgttaacaat gtgtgaattt ttaccagatt gacatttttc tgaaaataaa
      721 gtaagattat cttaatattc aaaatatttt caaagggggt aatttagtgt gagtaagcag
      781 atgagagata tttttatagt tggttttgct ttattttcta tgttctttgg tgcaggaaat
      841 cttatgtttc ctccattttt agggatggaa tccggcaaag actggttaat tcctcttatt
      901 ggatttgttg ttgcagatgc tggtatagca atgcttgtta ttatagcaac agcaagatgt
      961 aagggaagca tggatgatgt acttaggaga gctggtaaag ggttagcaag agtaatgagt
     1021 gtagctgctc tagcatgttt agcacttctt gtaataccaa gaacttgcgc aacaacatat
     1081 gaaatgggta ttatgcctat atttggagac catggtccag tagctaaagc tgtattttca
     1141 ataatcttct ttgggctaac tttagcattt actattaggt cttctaaggt tatagacata
     1201 ataggtaagt atttaactcc agcgttatta ttagtattat ttgttttaat agtaaagggt
     1261 ataatttctc cagcaggacc tatgagtcct gagcatatga tagataaaaa tctatttggt
     1321 gaaggtatat cacaaggtta tcaaacaatg gacgctctag gtgcagcatc tatggcaact
     1381 ataatgttga tgtctataat agctaaagga tatacatcag ataaagacca aataggaatg
     1441 actattaaag ctggttttgt agcctgtgta tttctagcag tagtatatgg tggtcttact
     1501 tatttaggtg caacagtttc aactttatat gatactagta tacctcaagc atctttatta
     1561 gttgaaataa ctagtatatt gttatatggt caagtaggaa aggtaatttt aggtgtaatt
     1621 gtggcacttg cctgtctgac tacttctaca ggtttaacag catcaatatc aacttatttt
     1681 gaaggaataa gtaaggcgag ttataaacaa ctggttatag gtatatgtgt agctagtatg
     1741 cttatatcaa atttaggtgt tgattctata atagccatat cagttccaat cctacaaact
     1801 atatatcctg ttttattagc aatagttgtt atggagttgg ctggtaaaca tataaagaat
     1861 gataatgctt ttaaaggtgc agcatatgtt acattagtga ttagtttatt atcagctata
     1921 aatggaatga caggagctgt tccatttata caaagcttgc cacttgcagg tttgggcttt
     1981 aactgggtaa taccagctat attgggtgga atagttggta actttataaa aagcaacaaa
     2041 caagtagcat aataatttgt aattaattta attttataat atattaattt ttagtactat
     2101 gggtaggttt aatctaaacc taatctaccc atagtacttt ttagattgga ttataatagc
     2161 atgtatataa ttttttataa tataattttt ctttgaagta tgataaaatt agtaatagtg
     2221 ataagaatgt acaaatttag gaggttttaa ttaatgctta gaaaaataac agcatttatg
     2281 ctatccatat gtttaattat tggacttaca ggttgtagtt tacttgtagg caagaaagat
     2341 aaagagaaaa ttatagatga ttttaaagtt gcagtggtaa ctcaaccatt atctgaaaat
     2401 aaagtacaat acaatatggt ggaagagatg gcaaaagaat atgaggaaga gaataaaatt
     2461 gataaagaca aagatggtca aactaaagtt aaacaaacta taaagcatgt tgtattgcca
     2521 gagaatttta cttctaatat agacagtgct attaataaga tagtaaagtt ggctgacgat
     2581 aaagaagttc aggctattgt agtttcaact gaccaagcag ggcttttacc tgctttacaa
     2641 aaggtaaagg aaaagcgtcc agaaataata actatttctg cccctatggg agatgataaa
     2701 aaccaattaa gccaatttgt tgatgtgaat ctaggggtta gtgcagagga aagaggaaaa
     2761 gtattagctg aacgttcaaa agaaatggga gcaaaagctt ttattcatta tgcctctaca
     2821 gatgatttga aagatgttaa tatagccaaa agattagaaa tgattaaaga gacttgcaaa
     2881 aatataggat tgccatttgt acaagttaat actccaaata taaatactga agaagacaaa
     2941 aacaaagtta agcagttttt aaatgaagat attgaaaaac aagttaaaaa atatggaaaa
     3001 gatataaatg tatttggtgt aaatgagtat atggatgaag ttatattgac taaggcttta
     3061 gaattaaagt atatagttgc agagcaaagc aatccatcac caatacaaac atatcctagt
     3121 gttatgggtc taaaaatatc tgaaaaagat gctcaaaatt atgataaaat aaacgacatg
     3181 ataagtgaaa aggctaaggc ttttggcatg tcaaatagac ttggtggata tcctatgcca
     3241 atggatgctt ttctaccatc agtagcaatt tatcttgcta ctgaaatggt gaaacaagat
     3301 ttaacacaag aagatgtatg tgacccagat tatttagagg cgtttacaga attaagattt
     3361 ggaatcggtt cagaatttac tcctttaaca gaggtacttt ataattatca atcagtaatt
     3421 ttaagccaat taatttatta atattaagga cgtgtctcaa aatgaacttt ttagtttatg
     3481 aggcactctt tttctgttaa atcaaatata tttttatctt tttaacaatt aaaaagaagc
     3541 ttatccatat gttgagatag cttcgttttt aatataaata agttttatta ttttataaaa
     3601 aatttataga aaaataaaaa attataatta tatataaatt ttgataaagt aaagatgaat
     3661 tactataggt ttaaattata ttattttcta ttatttttat gcctttctca gttattgatg
     3721 agccttttct tcccttacta gaaattaagt atccattgcg tgtcaaatat tctagcatat
     3781 ttcttacttc gtattctgtg acattatatt ccataaattt cattaacttt gtcctcccca
     3841 ttggtttcat gtgcaatgat tttaggatag aaatgcatat gtttttgtca tattggttta
     3901 aatcattgaa tatggacata ttacttgaca tatgattatt atttaatttt gaagatatgt
     3961 tttgaggtaa atcatttatt gtaagataat tggaacccat gtaagccata tattcaacac
     4021 aatttctcag ttctctaata ttccccatcc aagtgtatga atttaaagct tctaaaagac
     4081 tcttatcgag ttttatgttt ttatagggaa gttcttttaa aaatgattct aaaagaagag
     4141 ggatatcttc taatctcttt ctaagaggag gtaaatctaa agtgaacata cttatcctat
     4201 aaaataaatc catcctaaat ttattttcta taattaattt ctctaaatct ttatttgtgg
     4261 cagctaatat tctcacatca attggaataa tacttgtacc accaattttt ctaatttctt
     4321 tttcttgtaa gactcttaaa agtttaacct gtaaatcatg tggcatatct cctatctcat
     4381 ctaaaaatat agtgccagta tgagctaatt cgaataatcc tttttttcca cctttttttg
     4441 ctccagtaaa agaaccatct tcatatccaa atagttcact ttcaagtaat tcacttggta
     4501 atgaagcaca atttattgct acaaaaggtt tattttttct atttgaatga ttgtgtattg
     4561 actgagtaaa ggcttcttta cctgttcctg tttcacctgt tattaatata ggattatctg
     4621 taagagccat tttttttgcc tttttaatac aatcttttat aatagaacta tttccaataa
     4681 tattattaaa tttatattta gctacataac ctcttttgac agaatcactt cttatttttt
     4741 gttctaatat ttctatattg tttacatctt ttaatgtaat taagcaaccc tctatatttt
     4801 tataaagata aaatggtttt ttagaaagta tgaatttttt atctatttcg tctatgtata
     4861 taatcatatt atctatattt aagttctttg taattttatc taagaaactc tttggcatat
     4921 attccattaa ggaaggtgat tttataagtt tttcatctag ttccaacata ttaagtaaag
     4981 acttatttac aaaagtaacc ttattaaagg tattgaaaaa tattagacca tcacctattt
     5041 catcaatggt catgtaaagt atagttttta atttagatat attatctaat gatgtattta
     5101 agaaatcatg acttagtgtc tccttggaca atttgtataa tttttctata aatttctcat
     5161 tttttagttt taatactact aataatgaca tatatgtatc taatgagatt tttctccaac
     5221 ctaaatcaac aatatttttt atgtattttg gtataaatct aatctgacct ggtgttatag
     5281 ctgtatctaa atcatatatt tcatcacaat ctggatatgt cggtacaaaa tctatatgat
     5341 ttataccaat tttatataga taagtagcca tctcaaatgc tgaagtttta ttggttgtca
     5401 taagtaacga ttttgtacct ggaggcagtt tcatcatttt ttcatatcca agttttgaaa
     5461 gtgctttcgt tccatgaata atttcaatat cttccttaaa atgttttatt aaaaaattac
     5521 tagttaaagg tgatagagaa agcactacat ctccctctaa attttctttt attccatcaa
     5581 gtatattttg tgtttctatg attatttcgt ctccaaaaaa gtttgttaat tcttctttgt
     5641 agtggtcact attcaattta tcatatgtta taaggattaa cttcttttta tccattttat
     5701 ttctcctaac aaaatagtat ttatttttaa agtatttaaa gtataaggca agttatatta
     5761 tacaacaaat agtagtataa atttatatat ttgattaaat aataggtttt tttaggattt
     5821 tatcctataa aaacctattt tgaaattatt tttgcaagat aaggaaaata gctttttata
     5881 ttgtaatgta tattttaaat aattaataaa aaaatattat atttttaaac ttgaaaagta
     5941 agtaattata agctatttaa tcactggaat aaatttacaa taaaaaaata aatacaaagg
     6001 ctttatcatt aaggtattgg cataagtttt gcttattaaa caagtatact aataaaattc
     6061 atttaaacag gagggagaat ttatatgagt aaattagaat caacaacaga aaaagttgag
     6121 gtaaaatggt atgggtatgt agcattaata ttgggagcat tatttttttc aggaatattt
     6181 aaagatgcac caggaccatt aaaagtattg gattttaaca atgtattagg ttcttttgga
     6241 acattgggaa caattaatga tggtgtaggt acattagctg caaactttag aggtgatgga
     6301 ggtactggcc ctcgagatgg atggctatat gcattgacat taataccatc agttatgttt
     6361 gcacttggag tagttagagt tatagaccac ttagatggaa tgaaggcagc tcaaaagctt
     6421 cttagtccac tattaaagcc attattaggg ttacctggat ttgcaggact aactttgata
     6481 gctagtttac agtctacaga tgcagctgca tctatgacta aagaacttaa agatgatggg
     6541 tatatagatg aaaaacaaaa agctgtattt tgtgcatttc aattttcagg tgcgtctgca
     6601 attacgaatt tctttgcttc tggagcagca ctatttccat ttataggaga tgtaccaata
     6661 tttataccac ttgctttaat attaattatg aagtttgttg gggcgaactt acttagatta
     6721 tatttaaata aatttgacaa gggggaagct taacatgagt gaggtagcaa aaaaagaagt
     6781 aaaagttaaa aaaggaaatg tgatagatgc gtttattgga ggagctagaa atggattcca
     6841 aataagtaca aattcaatgg ctccaaatgt catcttcggt tttgctataa tttctgtgtt
     6901 caacttaaca ggtcttttgg atgtaatagg gacaatattt acacctgtaa tgagtatctt
     6961 tggacttcca ggtgttgcag caacagctat aatgacaata tttatatcaa tgggaggagc
     7021 atctggagta atcgcaggtt tatttacagc aggtcaaata agtcaagcac atgttgcaat
     7081 tctattgcca atgttattgc tttcaggagc gcagatacaa tttatgggga gacttttagg
     7141 aacagctgag ttaaaaacta aatattatcc acatatgttt gttatagcaa ctttaaatgg
     7201 ttgtattgca atgcttataa tgaattattt cgtagtatag gtaattataa taaaaattta
     7261 atataaatgg aaagtttaaa ttttaaggag gagctagatt atgggaacta acaaagatta
     7321 tattgagtat gtacataaag tgtatgatta tctacacact ataccagaaa aaggatttga
     7381 agaatataag acatcagagt ttgttttaaa agagcttgaa aaaatggggt ttgaaaatat
     7441 taataaagga gttgcaggaa ctggtattat agtaactata gattctaaaa aagaaggacc
     7501 agtatttgct ataagagcag atatggatgc attggaattt atcatagatg gagaaaagaa
     7561 aatgatacat gcttgtggtc atgatggtca tacttctatg cttttaacag ctgcaaagtt
     7621 aataaaagag agaaatttag taaaaagagg taagttaaaa atactttttc aaccaggtga
     7681 agaaaaatta tatggagcat taagagtaat tgattctggt ttgttggatg atgtagaaga
     7741 aatggtaggg atgcatttaa gacctggaca agattgtagg ttgggaaagg cgatacctgc
     7801 aatgtaccat tgtgctagct gtataatgga atttacgatt aaaggtatca cttgtcatgg
     7861 ttctaaacca catttaggag taaatgcagt agatgctgtc tgtttagcag tgaatgcaat
     7921 aaatgcaata agagtaaatc cagctatacc aaattctata aaagtaacaa atatatcagt
     7981 taagggagaa acttataata atataccaga tgaagcattt atggctttag atataagggc
     8041 agaaagtaat gatataatag aagaattaaa agaaaaagca atacatgctg taaaaactgt
     8101 tgtaagttct ataaatgcag aagctgtact aatttccaaa gatggagtac cagcagcaga
     8161 atttgatgaa gaacttgtaa acttggctga agaaactata aaagatgtat tgggagctga
     8221 tgcatctata ggtatattta aaaattctgg tggagaagac ttccattatt atacacaaaa
     8281 actaaaatgt aaaactacat atattggatt aggagctgat gctacaccag gatttcataa
     8341 tccaaatgta acatttgata cgaaagcttt agaatatggt gtagatatat ggtgtagatt
     8401 ggttgaaaag agattgggat aacatccaaa ctaataattc tacatttaaa attataatta
     8461 tagaagttat agaaaaagat tgcttatgtt aattagaaaa taattaatat gagcaatctt
     8521 attttgatat aatgaaactg attttggaat attgaaaaaa tatgatggaa aagtaattat
     8581 attttaaatc tattggacaa aaaagtgata ttattataat attaagaatt gtaaaataat
     8641 accaaagtgc atctagtaat agacttaaca attataaaaa ttttattctt aggagggaaa
     8701 aaatgagtaa gtataatttt gacagagtaa tagatagagt aggaacagat tgtgtaaaat
     8761 gggattttag aactaattgt tcaacaaaag cacaaaaaga tggtttgcct ttttggattg
     8821 ctgatatgga ttttgaatgt gcagaaccaa taatagaagc attacgtaaa aaagttgaac
     8881 ataaaatatt tggatatagt tcaaatgata gtgataaata ctttaatgca gtttgtgatt
     8941 ggtataaaag gagatttaac tgggaaataa atagaaaaga tattattttt agtcctggtg
     9001 ttgtacctgc tgttgctatt ttagtaagaa tattaactaa ttctaatgaa ggtgtaatag
     9061 tccaaaaacc agtgtattac ccatttgaag ctaagataaa gagcaataat agggacgttg
     9121 taaacaatcc tctaatatat gagaatggta cttataggat ggattatgat gatttagaag
     9181 aaaaagctaa gtgtagcaac aataaagtac taatactttg tagtcctcac aaccctgttg
     9241 gaagagtttg gagagaagat gaattaaaaa aggttgtaga aatatgtaaa aaatatgacc
     9301 tatggataat agctgatgag atacattctg atttaattag aaaaggattt aaacatactc
     9361 ctttacagtc attatgccca gagtacaagg ataaaatagt tacatgtata gctccaagta
     9421 aaacatttaa tctggcagga atgcagttgt ctaatataat agtgaataat gatgaactaa
     9481 aaaagaaata tcaagaagag gttacagctg ttggagttgc aacttcacca aatccctttg
     9541 ctattgtagc tacaatagca gcatataatg aaagtgaaga ctggcttaat gaattaaatg
     9601 attacttaga taataatata caatttattg atgaatactt aaaagaaaac ctaccaaaag
     9661 taaaacttgt ttatccagaa ggaacatatc tagcatggtt agattttact gcttatggat
     9721 taaatgaagt agaacttgaa gatttaatgt ttaaaaaagc taatgtatta tttgatgaag
     9781 gttatatatt tggaaaagaa ggaataggat ttgaaagaat aaatgtagct tgtccacagt
     9841 ctttattaaa agaatgtatg gacagattaa agactatatt tgaaaattta taaagtatag
     9901 gtaaaaattt ataaagtgtt taaaaataaa aaaataataa acttgacaaa ttttaactaa
     9961 tcacttatca tactattatg aataaataga aaacataaga gagctaaatt ttattgaatt
    10021 aaactcttaa agactttgaa aggaagagta gttatagaat gtaattaaag cgagttggtg
    10081 atggtgtgag accaatatga agtctataat gaagaacatc ctggagttgc taaccgaaat
    10141 tttaaaagag taggcttagt cggaaccaaa ccgttatcat attggggtgt atcgagtaat
    10201 atctgtacat tgtgagctgg gtttgaattt atttcaaact aatcagggtg gtaccgcgga
    10261 aaagcaacct ttcgtccctt ttatataggg atgaagggtt tttttaatgg aaaaatatat
    10321 aaatataggg agtgaatgat atgagcacaa taataccaaa ggattataaa agtagtttga
    10381 acgtaattga tacacaaata gctataaaaa aattaaaaga tttttttgaa atgagactaa
    10441 gtaatgaatt aaatcttata agagtttctt ctccgttatt tgtattgcca gaaacaggtg
    10501 caaatgataa tttaaatgga gaaaaggctg taagctttga tataccattt atgaataaaa
    10561 atgcagaaat tgttcaatca cttgcaaaat ggaaaagatt agcccttaaa aaatatgggt
    10621 ttgaagttgg tagtggttta tatacagata tgaatgcaat aagaaaaaat gaagaattag
    10681 acaatataca ttctctttat gtagaccaat gggattggga attggttata gataaaagta
    10741 gtagaaatga aaagactcta aaagatgttg taaaaagatt gtatggagta tttaaagata
    10801 ctgaaatttt tgtatgtagt atgtatgaag gtataaaaga aatattacca gaagaaataa
    10861 cttttataac atcacaagag ttagaagata tgtatccaga attatcacca aaggaaagag
    10921 aagataaaat tgtaaaagag aagaaagcag tatttataac tcaaatagga aaaacattaa
    10981 tatcaggaga aaaacatgat ggaagaagtc ctgattatga tgattgggaa ttgaatgggg
    11041 atatattatt ttacaatcct gttttagatt cagcattaga attatcttca atgggaatta
    11101 gagttgatga agaatctctt gatagacaat tgaaacttgc tggatgtgaa gagagaaaag
    11161 agtttgacta tcataagatg cttttaaatg gcgaacttcc gtatacaatt ggtggaggaa
    11221 ttggtcagtc aagaatatgt atgtatttct tacaaaaagc tcatatagga gaagtacaag
    11281 ttggagtatg gccacaggat atgattcaaa attgtagtag tgcaggaatt gaattattat
    11341 aatttaaata taaactaatt gataaaattt atatctaata tgttcatcaa attaattata
    11401 tcggttataa tgtagaataa cctataatta taagaggtga taaatatgga gtttttaaaa
    11461 ataatcatta atatagtttt agatatttta aaaaaaattt tggtcagatt taaaaatgct
    11521 aaatttggtt tggtttttgt atttgattta cttaaactac ctgattttat gacagataaa
    11581 agaatcaata tagttgataa aataaaggta atttctgtat taatttttac tatatcctac
    11641 tttgtatctg gggttgatat aattccagag atgattgcag gggcatttgg ttttatagat
    11701 gatgctatag ttttaatttg gagtataggt atagtgaatg aagaaattaa taagtataga
    11761 gttatcgcaa aaaaagataa acattcaaat ataattgaaa atgtagaatt ttctataaaa
    11821 gatgaagaag aataacaatt tatatatgtt attctttttt ttattaacaa aaaatggtta
    11881 aattgttgac ggagaggtaa aactctatta tcataataag tggatatcca atatttaact
    11941 aagtattcaa tgtgcaagaa tgcttaattt aaaaaagtta tggagggtaa atattatgaa
    12001 gaaaaaaata ggtttagtgc ccaaactaat catcggtatc atagtcggta ttttaatcgg
    12061 aagttatgca ccagaaataa tagtacaaat attagtaaca gttagtactt tattctcagc
    12121 attcctaaaa tttgttatac catttataat tataggtttt gtaacagcag gtatagctga
    12181 tttagcaaca ggtgcaggaa agttattggg gataactacg ggaatagcat atggttctac
    12241 cttaatagca ggattattat catttgcagt atctacatta atcttcccaa attttattga
    12301 tgcaagtgtt gcaagtcaaa ttggagaccc agaggcagga atgttagctc caatattcac
    12361 aattccatta gagcctatgg tagatgtaac agctgcaatc gtatttgcat ttgtaatggg
    12421 acttggaata tcagctttaa gaaatcatgg aaaaggagag actttattta atttcttcca
    12481 agaatttcaa ggaatcgtaa cgaaaacgtt gtcaacagtg attatacctt tattaccttt
    12541 atacatagca ggaacttttg caaatataac tatagcaggg gaagtatgga caatattagg
    12601 ggtattctgg aaagtatact tagtagtaat acctttacat tttgtatata tggcatgtca
    12661 attcacagca gcaggtgtat tttcaggaaa aaatccagta agaatgttaa aaaatcaagt
    12721 acctggatat ttaacagcta taggaactca atcttcagca gcaactatac cagttaatgt
    12781 tggttgtgca gaaaaaaatg gagtaagtaa gcaaataaga gaatttgttg tacctctttg
    12841 tgcaacaata catttatcag gtagtatgat atcaataaca ggatttacaa ctgcagtttt
    12901 aatgatgaat ggtatgccac atggaatagg tgtattattc ccttatattg caatgttagg
    12961 tatagcaatg gttgcagcac caggagcacc aggtggggct ataatgagtg cacttccttt
    13021 cctaccaatg gttggaatac catcagatgg tgggcttgca agtttaatga tagctttata
    13081 cttaactcaa gacagttttg gtacagcatg taatgtatca ggagataatg caatagcagt
    13141 agtagtagat aaaataaatg atggaatgag taaaaaagga aacaaagaag agcaaagtgc
    13201 ttagtttttt aataatcaaa aatgagttat ctcaaataga gatagctcat tttttatggt
    13261 ataaaaaatt ataaaataaa gtgatttttt tatttaaaaa ttgctttaag agatgatatt
    13321 aataatttat tttcattagg ttttaatata catactctaa agaaatactc atctagacct
    13381 tcaaatgaac aacaatctct tattattatt ttttgtggga gcagttgctc tcgaagagat
    13441 ttagcagtca attcctttgt cttgatttta cacaatatga aattaccttt agtattgtat
    13501 atatctaagc tttttatatt cttaagctct ttaagcaggt aatctctttc agtgttcatt
    13561 aaggaaatag tatttgatat aaaatctaaa tcactaaaca tgatttcacc cattttagaa
    13621 gctattatgt ttatattcca taaatctaga tttttattta tttcattttt gacattagtg
    13681 ttagatataa gaccatatcc aagccttatt ccaggcgttg aaaaaaattt tgatgtacct
    13741 cttataacga ataaattaga ataatcatca actaattgag tacaagagta tgtatctgta
    13801 tcagtaaact ctacataagt ctcatcaatc attaaaaatg agtctgtatt ttttaaaatt
    13861 tctctcactt ctaccttagt aaatgcaaat ccagttggat tactaggatt acagattact
    13921 accaaatcat aatctttagc attgatggtt tttataagat tttctaaatt tatatgaaaa
    13981 ttatcttctt cttttgcaaa gtatttttct atagaacaat taattttaga taactctttt
    14041 tcatactctg aataggctgg agagagtaaa agagcttgtt taggattaat tgtatgtata
    14101 aatgatgaaa ttagttctgt agctccactg ccaagtacta tattatctat agaacatttg
    14161 cagtaattgg atatagaagt ttttaagtct atgtaatcag ggtcaggata catagatacc
    14221 atatctatat tgtttacaat gtattgtttg gctaaacttg atgtaccaaa aggatttata
    14281 tttgagctaa aatccataaa ttcttccttt gaaaagccat acttactaga taaactatat
    14341 aaattagctc catggtttgt gctcataaaa gtacctccaa aaataaaatt atataatcga
    14401 taaaaataac tgattaatat atgtacatat agattataat acaaaggaat cgttattcca
    14461 aatttttaat gtaaaaagca aaaaagtatg gataagctgt taatttaggt ttaaaaatga
    14521 tgaaaaagat tttaattgat attttggtat gctataatat ataagttaat agaaggaaag
    14581 cgggaggata caaaatgtac aaagttacag gaaaaatgtt gacagaagaa caaataaaag
    14641 aaaaagttta tgaattagga aaaaagatag aagaagactt taaaggcgaa gatttactag
    14701 tggtaggtat acttaaagga gctagtgtat ttgtttcaga tttaataaga tgtatagatt
    14761 tggatgtaaa tatagatttt atgagtgtta caagttatgg aaactcaaca gagtcttctg
    14821 gaacagttaa aatattaaaa gatttagatg tagatattga aggaaagaat gtgttgattg
    14881 tagaggatat aatagattct ggtttaacat taagtaatct agttgcagct ttaaaaacta
    14941 gaaatccaaa atctttaaaa ctatgtactc tacttgacaa acctcagaga agaaaagcta
    15001 atatacctgt agactatgtt ggatttgtta tagaagacaa atttattgtt ggttatggaa
    15061 tagactatgc tgaaaagtat agaaatttac cttatatagg tatagttgag gatgtagaat
    15121 agatttaaat gtgaatcaaa agcactaatt tctaagggag ttagtgcttt ttagttaatt
    15181 atataaaatt ttagtaaagg agacatctat atggatattc agttaaatat attattattt
    15241 ttccaaaatt taagaaatcc aattttgaac tttatatttc ttgtctttac aataagcaca
    15301 gaagcaccgt tattaatatt aattacaaca attatatact ggtgtataaa taaaaaatgt
    15361 ggacagaaaa tactgtttgc tataattgga aattttgttg taaatttagg catcaaagag
    15421 tttgtaaaag ctcccagacc aataggaata aaaggtcttg aatcattgag agtttctact
    15481 gctggtggtt attcttttcc aagtgctcat actcaaactg caacaacatt ttgggttagt
    15541 attatgacta tatttaaaaa gagatatctt catttaatag ctgcagttat ggttttagga
    15601 gtaggattgt ctaggctgta tttagctgtt cattggccaa tagatgtgat agcgggttgg
    15661 atattaggaa tattttttac agttgtattt gtaaaaatat ttgactatat tgatgataat
    15721 aaaaattata ttttacttgt agttttactt ataccattta taatagtggc tatatttcta
    15781 aatagccctg aatattttga aaaatttggt ataatagtag gatttgtatt tggatatatg
    15841 gttgaggata gatttgttaa atttaataca gataataata acaaaaaaat aaattttagt
    15901 aataaaaata aggggagtaa aaatataatt ttttcaagaa tatgtaggtt tttggttggg
    15961 atagtaacaa taggaatgtt atatattggc attaagttgt ttatttcaat gttaatagtc
    16021 actttaaata taagtgactc tactatgagt ataattttta tgaattttat taagaataca
    16081 gtagttgttt tttatggaat agcaggagct ccagcattat ttaaactttt aaaattaaat
    16141 tgattttaag gtatagagtc aatagtttaa attaaaaaaa ttaagggtag aaaagagtga
    16201 ttatttgaag aagatagtaa taataggtgg tggaccagca gggatgatag ccgcatcaac
    16261 tgcatgtgag aaaggctatg atgtaacatt aattgagaaa aatcataaat taggaaaaaa
    16321 acttgcaata acaggaaaag gtagatgtaa tattactaac gcttgtgaaa tagaggaatt
    16381 aatagaaaat gtacctacaa atggaaaatt tctttatagt gctttttata cttttacaaa
    16441 tgatgatgta ataagtatgt ttaataatct tggagtaaag actaaaacag aaagaggaaa
    16501 aagggttttt ccagagagtg ataaagcatt tgatattgta aatgctcttg aaagacaact
    16561 taaaagtaag aaagtaaata tattattaaa ttcgaaagta gaaaaaataa tatctaaaaa
    16621 taataaaatt gagaaagtaa ttttaaacga taaaaaagaa attaagtgtg attcagttgt
    16681 agtagcaact ggtggattaa gttatccact tactggttct actggtgatg gatataaatt
    16741 tgcaatttca caggggcaca caattataga tacaaaacca tctttaattg gtatagaagt
    16801 acaagaaagt tttacaaaag atttagaaaa attatcattg agaaatgttg aaataagggt
    16861 gtttaatagc aaacaaaaga aagtttatag tgattttgga gaacttgaat ttactagatt
    16921 tggtttagat ggacctataa tcaagtctgc aagttgtaga atgaaagata caaggaaaga
    16981 aaattacact atattattag atttaaaacc tgcattagat gaggaaaaac tggataaaag
    17041 agttcaaaaa gattttcaga agtatacaaa taaaaaattt gaaaaagcat tagatgattt
    17101 acttccaaaa aaactaatac ctataataat aaacttaagt gaaattaatc caaatacggt
    17161 tgttcaccaa atttctagag agcagagaaa aaacttagtc catttattaa aaaatttaaa
    17221 gtttacagta aagagataca gacctattga agaagctata ataacttctg gtggtgtaaa
    17281 agtaaacgaa ataaattcaa gtacaatgga atcaaaatta gtagaaggtt tattttttgc
    17341 tggagaagta atagatatag atgcatatac aggtggattc aatttgcaaa tagctttttc
    17401 tactggatat ttggcaggtt ttaattgcta aaaattacct gaattaaaaa tgtttaaaat
    17461 cacataatat tatcagcaac acaaaataaa ttaaacagaa ggaaggtaat caacatggca
    17521 agtaacaata acaacaacag aacagtagta ccaggagcaa aagaggcttt aaaccaaatg
    17581 aaattagaaa tagctaatga acttggtatg agtaattatc aacaagttga taaaggaaac
    17641 cttactgcaa gagaaaatgg ttatgtaggt ggatacatga ctaaaaaatt agttgaaatg
    17701 gctgaacaac aaatggctgg aaaaagcaac agatagttat aaataattat ttataactat
    17761 tggctattag tttagctaga gctatctcaa atatagaaag taatttctaa attgagatag
    17821 ctttttttta ttaaattatt aaatagatat atttttatgt aataaatttt atgtatttgg
    17881 ataaaataat acaaatgaat atgttacata aggagtgatg taaaatgagt agtgtaaatt
    17941 ttgaggattt aaaaaataaa tttattaata gtgacttaga tgaaaaaata agaatatata
    18001 caacaacaga aggtttatca gtagaacaat ttaaagagct actaaaatat tatccaatac
    18061 aacatttatc taaactagaa aaagcacttg gatagcagta tttaatccaa aattaaaaag
    18121 gcaatttatt taatattaaa aatatttttt attaaataaa ttgccttttt ataataggtt
    18181 taaattatta tgcttttgat atataattgt agattctctc atttccaagt tcaatagacc
    18241 cagtattaat aagagtaacg attccttcta tatctctagc agttaagtat ttatatggaa
    18301 ttaataaatt ataaccagta ttacttagta agttttttaa ttgggttatt tgagagatat
    18361 tgaaacattt tgcagtttta gtagattttt ttcttaagtt agataaattt tcattttgaa
    18421 tatttattaa agtttctata tagtttctat aagatatctt aaagtttgtc tcattgacta
    18481 ttttttcatt aagtatacta ctgataaaat cttgagacaa tatattatct gcaaatttat
    18541 ctgctgttaa ttttccagat gttatgcctt tttgatattt tctaagaatt tttttagtat
    18601 agcagtatat tttatttctc gtttctaaat cgagttgttc tatttgcaca aaaccacttc
    18661 cttctaatta taaaaatatt tattaaaaat ctatacttaa ctaataaagc ttcaataact
    18721 tatttacaat aaatactaaa tcataaaata tttgttattt tttattatta ccaagtataa
    18781 aaaatattat aattgtaaaa cttacaaata tatgtaaaat atttaggagt gatgaaaatg
    18841 ttaaatggga taataaagct tttttattct gaaattgtaa aaacaaagat taataactta
    18901 gattcaagtg gatattcttt aagagatgaa tgtaaagaaa tcagaaatat aactctaaag
    18961 atagatagtg tattttttaa gcttttaaag ttagcttttg gttcaatatt gcttatggta
    19021 gcttttaatt taaacatggt tttaggtata ggagtgtttt tagttggtgt agtttatata
    19081 ctatataaaa gaaatatgga agaacaagta aaagagcata taaataatgt gaaaaataat
    19141 atcgacatct ctaaaataac ttttatggat gaaaaaggga gaacaggtct aaatgcatta
    19201 attacattac ttcttatagg attattgaca ggatttaact ggatattagt tgctagtttt
    19261 gtcgttgttt ttgtttttac tataaagaat gtatgttaaa atactaataa atagtttaat
    19321 atataggggg agaaattgag tatgactaat ggcagttata aaatgaagaa tttagtggaa
    19381 atatccgcta tggtgactga atctacagat ttttttaata taaaagatga cataatagaa
    19441 aaaatgttag aagttgttca ccctacaaag gcatgtgtga atctatttta taaaaatgat
    19501 tctaaatatg cctacttagt atgttcgcaa accttagaat atgtacctca aatttttcca
    19561 ataaatagtc ttaaaggtgc aaaaattgat tttgacacat atccagagta tatacatgag
    19621 gctgtaaatg aaaaaaaaat aatatatgtg gaaaatgtat ttgaagattc tagagcagaa
    19681 ggtgaacgag atttagcaaa agctgaaggt tatattggga gaatagtttt tccttttata
    19741 ataaataacg tagttgttgg atttatgact tgttttttaa aagaggatga ttatataaca
    19801 gaacaagata tagattttat atcatctgtt gcatcactct taagtttatc aatagaaata
    19861 actaataaaa ataataatgt acaaatttta attgataaac taagaggctc aataagtgcc
    19921 ataaatgaag ctactaaaaa actatacctt aataagagta ttgatggatt tttggaaaat
    19981 ttaagtaaac aagcgtgtaa tattactaat agtaaagaag ctttaataat tataaatagt
    20041 catcataata aggatgctat agttagttgt tataatgttg agcaaaaaaa gaaaacaaat
    20101 ttatatccta tgattgatat gtttttaaag aaagagtcat tgggggggta tttaaataat
    20161 ttaaaaccat gctcaaaaaa gggaataaat ttggaaagtt atatatatta caaacttcag
    20221 gataaaaatg agactatagg atgtatagtt tgtgcaaata gcaaaaacta tactagtgat
    20281 gatttaaata ttcttagtat attgtccaaa caagtttctg ttggtatgca attatatgaa
    20341 cataatgaaa aggaagtaaa acacaaagtt cttgaaaacg agttgaacat attaaataaa
    20401 caacaacagc ttattatgga taaaggtaaa atggattgta atgatgagaa agaactttat
    20461 ttttaccata aaccagcaag ggtagtaggt ggagattttt attatgctac aaaagtggat
    20521 gatgaacata tagtttatat tgttgctgat gtaatgggac atggaatggt atcaaattat
    20581 gttgttgcaa tgataaaagg tgcttttaaa gtattatgca atcaatacaa tacaccaaga
    20641 gaaataatga caaatttaaa taggatgtta tatgatgaat ttgacaagat gggagtattt
    20701 acaacttgtt tgataggtat tatagatact aaagaaaata tgattactgt gtctaatgca
    20761 ggtcactata gtcctgtagt tgtaaagaaa gatggcacaa tagaaaataa tttaaattgt
    20821 aaaaagggca tacctattgg aataatggaa gatgctactt atgaaaataa tactctttca
    20881 atggaagatt atgcaatggt atgtatgtac actgatggaa ttttagagat aaaaaattca
    20941 tcaaaagaag agtatggtat aaacagatta gaaaactttc taaaagaaaa ttttagatta
    21001 aatcaacagt taatagtaga aaatctaaaa ttggatttaa agaacttctc atcaaaagat
    21061 aattatgatg atgatatttt aatagttatg ttaaaggata ggtagattga ttatggattc
    21121 aaacgataaa aaaggactta aaagtccaga gattataata ataaatggaa gtccaagaat
    21181 taataaaaat tgcagtttta tagcaaatga agtaaaagct atgctagaag agtgtggatt
    21241 aagttgtaaa atatttaata tctacaatat ggatattgaa tattgcacag catgtggatt
    21301 ttgtgaaaaa acagggtatt gcaagtttaa tgatgatatg aagccattat ataaaatgtt
    21361 tgatgattca aaaggaacta tagtaataag tcctgtacat tttgattcaa tatcagctaa
    21421 attaaagact gttgttgata gaactcaagc tatatatgca agtaaatatg tattaaataa
    21481 accttcaatt gatagaagta agaaacggat aggaatgtat attgcagtgg gagggagtaa
    21541 atcatatgat tcacagttta aaggtggaca gattgttatg gattttttct ttaagagtat
    21601 aaatactaaa cttttacaca atatatattt aaacaataca gatgatgtat cttatataga
    21661 taatcaagaa tttaaagata taatatataa aaatttaaag atatatattg attcaatata
    21721 atttgatttt gtaggtaata ttatgtataa ttgtattgca ataatttaaa taattaaaag
    21781 gagggaattt caatgaaatt accaatagca ttatctaata aacatgttca tttgagtcaa
    21841 gctgatatag acgtattatt tggagcaggt catgagttaa ctaaatttaa accattatct
    21901 caaccagggc aatatgcctg tgaagaaaaa gtggatttag taggaccaaa aggaacttta
    21961 aaaggagtta gagtactagg accagctaga gctcatactc aagttgaaat atctttagca
    22021 gatggtttcc cattaggagt aaaagcacca ataaaagaat ctggtgatat agctggaact
    22081 ccaggtgtta aattagtagg acctgctgga gaagttgaaa tgaaagaagg agttatagtt
    22141 gcttctagac atatacatat gagcaatgaa gatgctgcta aatttggtgt aactgataag
    22201 caaatagtaa aagttaaaac atctggacca agagcattag tatttgaaaa tgtattagta
    22261 agagctagcg acagctttaa tctagaaatg catgttgata tggaagaggg aaatgcagca
    22321 ggcgttaaaa atggagattt agtagaatta atagttgatt aatattataa atagacttta
    22381 tgctagtaga tattttattt gctagcatat ttttaaatgt aataaatttc tataggataa
    22441 atttaatcga ttagattaat ttataaaata aaggggtatt ataaaaaatg taataaaaac
    22501 ttacaaaaat atgatgtaat atggtaaaat tatatatata aaaatttaca tgtaataagg
    22561 ataagcattt tactttttat actaatatga aca
//
LOCUS       DAEMIK010000059        22546 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEMIK010000059 DAEMIK010000000
VERSION     DAEMIK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.059"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1026)
                     /locus_tag="KPK87_001891"
     CDS             complement(<1..1026)
                     /locus_tag="KPK87_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG2538042.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISII"
     gene            complement(1174..1965)
                     /locus_tag="KPK87_001892"
     CDS             complement(1174..1965)
                     /locus_tag="KPK87_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2538043.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(2001..2657)
                     /locus_tag="KPK87_001893"
     CDS             complement(2001..2657)
                     /locus_tag="KPK87_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538044.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2650..3615)
                     /locus_tag="KPK87_001894"
     CDS             complement(2650..3615)
                     /locus_tag="KPK87_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG2538045.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3721..3827)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4117..5061)
                     /locus_tag="KPK87_001895"
     CDS             complement(4117..5061)
                     /locus_tag="KPK87_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG2538046.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNVVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKCCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            5172..5639
                     /locus_tag="KPK87_001896"
     CDS             5172..5639
                     /locus_tag="KPK87_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538047.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICILSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(5809..6408)
                     /locus_tag="KPK87_001897"
     CDS             complement(5809..6408)
                     /locus_tag="KPK87_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538048.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            6654..7250
                     /locus_tag="KPK87_001898"
     CDS             6654..7250
                     /locus_tag="KPK87_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG2538049.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(7348..8307)
                     /locus_tag="KPK87_001899"
     CDS             complement(7348..8307)
                     /locus_tag="KPK87_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2538050.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(8341..9072)
                     /locus_tag="KPK87_001900"
     CDS             complement(8341..9072)
                     /locus_tag="KPK87_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538051.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(9065..9835)
                     /locus_tag="KPK87_001901"
     CDS             complement(9065..9835)
                     /locus_tag="KPK87_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538052.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(9920..10630)
                     /locus_tag="KPK87_001902"
     CDS             complement(9920..10630)
                     /locus_tag="KPK87_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG2538053.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(10810..10962)
                     /locus_tag="KPK87_001903"
     CDS             complement(10810..10962)
                     /locus_tag="KPK87_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG2538054.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(10988..13144)
                     /gene="feoB"
                     /locus_tag="KPK87_001904"
     CDS             complement(10988..13144)
                     /gene="feoB"
                     /locus_tag="KPK87_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2538055.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(13244..13465)
                     /locus_tag="KPK87_001905"
     CDS             complement(13244..13465)
                     /locus_tag="KPK87_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2538056.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(13478..13690)
                     /locus_tag="KPK87_001906"
     CDS             complement(13478..13690)
                     /locus_tag="KPK87_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2538057.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(14011..16908)
                     /locus_tag="KPK87_001907"
     CDS             complement(14011..16908)
                     /locus_tag="KPK87_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2538058.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(17235..17843)
                     /locus_tag="KPK87_001908"
     CDS             complement(17235..17843)
                     /locus_tag="KPK87_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG2538059.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISCQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKCLA"
     gene            complement(18103..18648)
                     /locus_tag="KPK87_001909"
     CDS             complement(18103..18648)
                     /locus_tag="KPK87_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG2538060.1"
                     /translation="MKKFVCTVCGYIHEGDTAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(18766..20589)
                     /locus_tag="KPK87_001910"
     CDS             complement(18766..20589)
                     /locus_tag="KPK87_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538061.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTILSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(20602..22377)
                     /locus_tag="KPK87_001911"
     CDS             complement(20602..22377)
                     /locus_tag="KPK87_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538062.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRKRIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         7478 a         3910 c         2240 g         8918 t
ORIGIN      
        1 aattatactt atatcgttat caaatttctt caatttttca gcaacttttt catcttcctt
       61 tataaaagtt aatattttat aataattatt catatccttt gaaaattcat ctttaaattt
      121 attatttgtg taacttatat taaaatcata gtctgttact atccatacat ttggtattgt
      181 atccagagct tgtctaagta attcttttct ctggtctatg tagtttttta atgttctttg
      241 tataatcata tccttttcta ttgctaaatt tatctttcct aattgctctg actttttact
      301 taatttttta ttgattgaat caagttcata atatggatac actactctat tattaagact
      361 gtatacatat acataataag tatggttaca taatagtaaa tatattactc ctattaatct
      421 atctgaatct actatattaa tcttagaaat tgctacaaaa catataatag ttgtaaaaaa
      481 aacaaaatat gttaaatctt ttattaactt atagtgctca atgttactat caagtttatt
      541 caacaaatat agtaaattaa acatagatat acaaatatta attaatataa taatcatatt
      601 ccagttaata tcactaaaaa tatttcttat aaagtaatct aatatcacaa aagcactata
      661 aaaaattagt attgggctta ttcttagttt ttttacttta gaaatggtta agtatataaa
      721 agtagacata ttaattatgc ttaagttaag caatacaatt tggaattttt gaatttttaa
      781 atttactact cctaaaatac ttacaaaaac taatacaaga caccctactt tcaatatatt
      841 tataaattca tctgttttaa agctacttat ataaactata gaatatccaa atatccaaag
      901 taaaattatt ctatacatat ttatagaaac tgtatacaaa tttatattta aaagtgcaat
      961 aaccaaaaat aacccattag ctagtatata gaccttattc ttacttaaag ttcctttaga
     1021 ttccatattc cccaccactt cttcattttt atatagtttt acctataaat atgttaccac
     1081 aattcttcca aaaaatttgt cactttctag ttataaaaaa aaatgagtaa atatttttaa
     1141 ttttactcat tttgtagatt ttgtatattc agtttaaaaa gctggaacta tacttccttt
     1201 atattcatct tgtataaatt ttttaacttc tttactattc atagcctctg ataaagcctt
     1261 aatcttatca ctatctttat tattttctct acaagctatt atatttacat atggagaatc
     1321 acttgattct attactattg catccttagt aggatttaag ttagctgtta aagcataatt
     1381 agaatttatt actgctccgt caacatcttt taatactgtt ggtaattgct ctgcattcat
     1441 ttcttttatt actatatttt taggattttt agttatatct tttttagtta taagctctcc
     1501 atcttttact tctatcaatt tattttttgc taataattta agtgctctag ctccattagt
     1561 tgcatcattt ggcacagcta taacagctcc atcttttaat tcatctaatg acttaacttt
     1621 ttctgaatag aatcccattg gctctatatg aactttagat gtataagtaa gtttatatcc
     1681 tttctccttc acagtttcct ctaaaaatgg aatatgttga aagaagttag catccaaaga
     1741 cccttcatct aaagctgtat ttggaagtac ataatcatca aatattttta cttctaaatc
     1801 atatcctttt tcttttaaca aaggttttgc aacttccaat atctttgcat gtggatttga
     1861 tgaagctcct actagtattt ttttatcctc tttgtttgaa caacctactg ctgatattgc
     1921 tattgctgac accaatgcta ctgataataa tttctttaat ttcattttta ctccccctct
     1981 ttaaaatgtt tataattaat ttattttagt ttcttataag ccagatttcc tactccctgg
     2041 attatctgaa ctaatattat aagtgctatt acagtatata ccattactgc tgtatcaaat
     2101 ctttgatatc cataaataag tgctatatta cctaatcctc cagctcctac tgcacctgcc
     2161 atagctgtat aaccaagtat agatattatt gataatgtaa ttccagatac tattgaaggc
     2221 atagcttcct ttatcataac tttaaatact atttgtcttt ttgtagctcc aaaagatttt
     2281 gctgcttcta tcaatccttt atcaacttca tttaatgagc tttctataat tctagcgata
     2341 aacggtgctg ccccaatagt tattggcact attgcagcag tttcccctat acttgttcca
     2401 acgattaacc tagttattgg aatcattgca actataagta tcataaatgg gaaacttcta
     2461 aatatattta ctataaatcc taatgcacta tttattgtgc tatttggttt taatccatct
     2521 ggttttgtaa ctactaatat gattgctaat ataaatccta atattgttgc cactatagtt
     2581 ggtactataa ccatatacaa agtttgtaac aatgcatttg gaaatagtgt tgttaaaaag
     2641 tcaattaaac tattcattta tcatttcctc ccatcttatg cccttactag ttagatattg
     2701 ttttacttgc tctccacttt tatctgaaat gtttataata agtgaaccta aaatatcatc
     2761 tttaaattgt tctaatttcc caaatattat agacacatct atatttagtt ctcttgccat
     2821 agtcgtaata atagcttcat tagaaatatc tttagggaat agtatcttta tatttgttcc
     2881 ttttggcaat attatacttt cttcacctat taattttcta agtcctttag tatttcttaa
     2941 gaatatttct tctgtatctc ctacctctaa tacttctcca ttttccatta ttgctactct
     3001 tccacaaatc tgctttataa cttccatttg atgagttaca actattattg ttattcccaa
     3061 ttttttattt atatcttcaa gcaatgaaag aattgacttt gtagtatttg ggtctaatgc
     3121 tgaagtcgct tcatcacaaa gaagtacttg tggatttagt gctaaagctc tagcaattgc
     3181 aaccctttgt ttttgtccgc cacttagatt tctgggttta tcatttttct tttcagatat
     3241 tccaactact tctaataact ctaaaactct ttttttaatt tctgcctttg agtatccaaa
     3301 acattctaat ggcaatgcta cattatcaaa tacagttttt ctttcaagca atgaaaagtg
     3361 ttgaaaaatc attcctaact cttttcttaa atctctcatt tgcttttcat tcaatgactt
     3421 tacttcttta tcagaaacaa gtacactgcc ttcttggtat tcttctaatc cgtttataca
     3481 tctcagtagt gttgatttcc ctgcaccact atgacctatt attccaaaaa tttctccaga
     3541 ttctatttca atacttacat ctttaagcac ttgaatcttt ccataatact tattaacatt
     3601 ttttatgcta atcataaaat ttcctcccat atattctgct cagtttttat gtttatcctc
     3661 tcttatgtat tacaacaaaa taaaagcccg agtataaact caggcttaga aagctattta
     3721 tactcatcta tcaactatat tagttgcagg atttagcacc aatgtatata aaatatactg
     3781 gttgccgggt ttcaaagggc cagtccctcc accactcttg ataagacgtt atttaattta
     3841 attctattat catttgttgc tatgttatat atgatatatt cattaatagt atttgtcaat
     3901 aaatttattt aaatttttta ttttttatta tcgttaatta actattttta attagttttc
     3961 tagctcctaa cactttatat tttaatgtct atataactat agaacatcat tataagaaat
     4021 aaattaaata acatcaatct atctaaatca tctgtaaagt aaattaagaa aattaacatt
     4081 ttatccaaac tctatcctac tattaaataa aataatttaa atacaatgat gattatatag
     4141 ctctagtact ttagacttta tcaaatgttt aagttctata ggttctatta ttgtagcatt
     4201 tttcccaaaa gttaaaaaat attcagccac ccagttaagt gtagattttg gtatatacat
     4261 atctatgtat ccacctttag acaagatttt caatcctctt gccaacaaaa attctgtttc
     4321 acaacatttt actccctcat ctgtcaaatt aattttaata tgataatctg tacctacttc
     4381 catattctta agataatcat gtatagaaga taaaacctta tatttcttac ttgataagtt
     4441 ttctatactc aaaatcttca caattctgtc tacacgaaat tccttaatag attctgattt
     4501 tatacagtaa gctggacaat accaaagtcc attcatggca tacaaaccaa taggaacaat
     4561 ggtacgtaca ctattttttt gctttgatga atattcaatt ttaattgtag attcattcat
     4621 tactacatta aacaattctt tcaataatgg tgttgaacag tgcctatctg gtgtccaaaa
     4681 aacaaattta cgccttatat tctcaatatt atattgaata tcatttggta tacaatttaa
     4741 aaattttttt aaaacagaaa tagtttcttg ctcaaatggc aaatcattat aatattctag
     4801 tgactgatat gcaaagaata tagattttac ctcactttct gaaaatgcaa tcggtggaag
     4861 tatacgctct ttaagtacat gatatccacc agccgctcca acttttgaat ataatggaaa
     4921 tccaatactc tccagcacct gtaagtctct tagtatagta cgttttgata cagaaaattc
     4981 ttctgctaat tctcctgctg taaaacttct ttttgcattt attgttatca tcagttcaat
     5041 taaacgctca gctcttgaca tatttatact cctaatttaa atatagtgac aataattgtc
     5101 actattatat gataagatat tcttacattt aaagcaataa taatagtaaa gaaagaaggg
     5161 actgatactt tatagaaaat aatactatta aggctgatga tattatcaat tattgtctat
     5221 caaacttaga tgatgtttta ttaatgaata gttgggggga acgagcaatt tactacaatc
     5281 ctaatggtat tttaaagcga ggagtatatg ttcttaccat taaagaaaaa gacagtaatc
     5341 atgataaagg ttcgttggtt agtcgtccaa atgtataccg tgtgaatata ggattaaaaa
     5401 aagaaacttt tattgaaatg tttggatata ttccaaagcg tccaggtgta ggtcaaatag
     5461 ttgatatgga ttttgatttt acaaaattgg acacaatcat gccacatcct atctactcat
     5521 ggatgggatg gatatgcatc ctaagcccta ctgaaaagac atttgagaac tttaaaactt
     5581 taataggaga atcttacaat tttgcaaaac aaaaatttaa aaagagaaaa aacaagtaaa
     5641 aacatatgaa aataatccct aaataaatta taataaaaat attaaggtta caagataaaa
     5701 attaatatct taataacatg caaaaaagcg aagccaaaaa atgtattatt tataaggctt
     5761 cgctatatct caaataattt atttcaaatt tcaaaactat ttattctatt atttcttatt
     5821 atttcctgtt attcctaatc tatcattcat atcttttatc atttttctaa aaccactacc
     5881 tatatcattt ccaacatctt cagttccatg aactaatttt ataaagtcat tttgaccttt
     5941 tttcatatct tcatcagata ccttatctaa tttttctatt ctcttatcta tgtcttttac
     6001 taatctttcc ccttcagcga ttaggtcatc attcattttc tttaactctg tatcttctgt
     6061 tttaccatct tttttaagag atgttacaaa tgattgtatt ttatctctac tatctttata
     6121 tgcagccttt acatcactta gatatttttc atttcctgga tattctttat ctttaaactg
     6181 attatttata ctttctggag tatcataaat agaatatcta tttagacctg ctatataatc
     6241 attatagctt gttgaataat aattcatata tccttcatta ttatttacag attcattagg
     6301 attttttcct gtattttcac ttggtttttc attattttta gtacatgcag ttatacctat
     6361 agctaatact agtgctaatc ctacaaataa agttcttttt aatttcattt ttattaccct
     6421 cctagatgtt ttatactata aaaaatttta ataattttct ttcttaattt agtatttcta
     6481 tatttttata ttttatttac taaaattaaa caagttataa tttatttttt tggaaatcat
     6541 aatgaataaa taattaaata aaaaaattat aattttatta ttgatattga ttatcattca
     6601 tagtataatc taattacttg atattaattt tcatttgaag gaggttatct tttatgtctt
     6661 gttgtaattc acaattttgt tgtaaaaata aaattgaatc atcatatata aaatggcttt
     6721 tagaagtttt aggtccagtc tttttaggct ctaaacctgc tgaaataata aatatttcgt
     6781 ttactgatgt aaatagagag gaaaaaatta atgatattta taaatatcta tataaatgta
     6841 agaaaataga ttttatagtt attgataaag aaaaaaaggg attaaaaatc ttatttgtaa
     6901 ataaaaaagc attatcagaa aaattaaaat gtaaaaaaac agtaaacttt ttaaagtttt
     6961 taggatacaa acaaaatacg aatgttaatg cttatttaga gcatttagta gataaactta
     7021 agagtgatgt ttttcctgat gaaataggta tttttttagg atatcctctt aaagatgtga
     7081 ttggatttat gggatatagt aattatgaag tttctatgat taaatattgg aaagtttatg
     7141 gtgacacaaa acaatccgag gacacatatt cgaagttttt acttcaccgt aagaaaatga
     7201 gaaaattact agactacata agtgtagata aaatagtaag ctgtttttaa ttgtattttt
     7261 gtataataat aaaaatacac cctcttttac catatagttt ccataataaa taagggtgta
     7321 ttttccactt taatatttta aagtattcta attttgattg tacaacatat ctttaatatt
     7381 tattttcttt tgttgcattc cattagacat taaaaattcc tgtgtatctt ctaattcttt
     7441 tatatccttg tccgttattt tagaactaaa gtcataccaa gaatacattt cttttgtttc
     7501 atctaaagat aaaccaactt ctttagatac aacatccatc acttcatcct tgttttcatt
     7561 catatatttt aaagtttctt catgaacctt cataaatctt tttactaatt caggatattt
     7621 ttcagcaaag tcatcactta cagcagtaac tattattcca cttactaatc cttctccatt
     7681 tacaatcatt ttggcacctg aatttatcgt tttcaatgcc actggacctg caagcattgc
     7741 tacatcagca cttttacttt ctaatgcaga agcagcacta ggaatatcca tatttacata
     7801 ttcaacatca tctatagtat agccttcttt agcaagtgca gcaatcaaca gttgatgaag
     7861 tatagtccct tttggaccta caattttttt accttttaag tcttttatag atttaatatt
     7921 atcattatta gttataagca tatatccttt tggtgaacgg ctataaatat tcgtaatttt
     7981 aagaccaaca ccatttgatg cagctattat tgcagacgtt cctcctaatg catgtaaaaa
     8041 gtctagctca cctgctgcta atgcttgagt ttgctctggt cctgttgtca attcatggaa
     8101 attgactttt atattgtcct ttttaaattc ctttccaaat aaatcatcct ttttttctaa
     8161 tatactagga acattaagtg gtgattttac ataagttaaa tttatttcct taggtaaact
     8221 agcctctttt tttgcaggtt catctttctt atcatttgtt gttgaacatc ctacagtccc
     8281 aaccaacacc attattaaag atgtcattac tgctaaaatc tttttgtttc ttttaatcaa
     8341 ttaaaactcc cccaattccc ttaatatttc ttttttcaag tttatataat agtttttagt
     8401 taaatctcta ttatattcat cttcgacact aaattgttta atccttttat ttttactaaa
     8461 aacaattatt ttctttgcta tttccatagc ttcttcaata ttgtgagtta caaatactac
     8521 acctttttta gtatttttat ggatatttac aacttctttt tgcatttttc ttcttgtgaa
     8581 gtaatctagt gctgcaaatg gttcgtccat cagcaaaata tcgggattga aggaaagtgc
     8641 tcttgctata cttactctgt gagccattcc tcctgataat tcatttggat atgagttttt
     8701 aaacttttca agcttcatca tttttaaata cttgtctaaa tcaactttat ttctattatc
     8761 tttttcagta tgtaaaagta tattttcact tacattcagc catggcataa gtctactttc
     8821 ttgaaatacc attcctactt ttggagtaca ctttttatca ttattaaaaa aatatatgtt
     8881 tccactagta gcattttcaa gccctgctaa aattctaagt agcgttgttt tcccacatcc
     8941 actttcacct aatataacag ttatctcttc actagatata tttaaagata tattatcaag
     9001 aactaaatgc tctttattat caactaaata tttcttatat atattctcta gcaaaaatcc
     9061 acttctaacc atttaaatca acctgcttcc ctttgctaaa ttttgttatt atctttgaaa
     9121 atagataatc tgaaagtata cctaaaaatc caataacaaa aattcctacc aatacaacat
     9181 cagttctaga catatctttt ccatcagaaa tcaaatatcc tagtcctgaa gaagctgcaa
     9241 taagctctgc tccaattata gccctaaaac tatacccaag ccctaaacta agtccaacaa
     9301 caatatccag catagaatta gggattatta ttttataaaa aattctcttt ttagataaat
     9361 taaaagattt acctacttct attaatttat tgtcacaatt ttttatacct ttcaatgtat
     9421 ttaaaaatat tggaaaaaag gatgccaaaa taataattat aattttagat tcttcaccta
     9481 taccaaacca taaaataagc attggcacca aagctagtgg aggtgtactt cgcataaatt
     9541 caaatagtgg tttgaaatat tcatacattt ttttatttac tccaaataaa actccaagtg
     9601 gtatagccat caatgaactt attaaaaaac ctattaatat ccttcttaca ctggcataaa
     9661 catttaagaa aatagaaccg tcatatatca tttttataaa agtatctaag actatttcag
     9721 gaggaggcag aatatagcta ctccatatac ccaaaacaca tactatcttc cataaaatta
     9781 aaattattaa aaaaatataa tatcctttaa gccttttatg aattttctca aacatataaa
     9841 actcctttta acatatctat ccctgctacc ctttttattt tttaatatct taaataataa
     9901 ttaagctata tcataagatt taatctatgc caagtcttaa tccacctgaa ctcattctat
     9961 cagaaggtgt agaatctgtt tttaagccag acaatctttc agatggttgt ataatgacta
    10021 tttgtccagg ttcaacaaat tccatatgat aagattctcc tgaagtctga ccaataaaag
    10081 tcttccatga tagttttgat actttaggat atggttctct tccagtccaa gctactaatg
    10141 catctgggtc aacacaacaa ggtccttcaa gaattagagg attaccatct gttattatag
    10201 caacatcttg attttgacct gttttattta ctaaatgtgt agtaaaaaga cctttttgag
    10261 atattacccc tgaaccaatc aattttacac tgtaatttaa ttgttcagaa aatgccaata
    10321 agttttcact ctcaacataa atggaatcac ctgggtcaat tgatattaca tctacatggt
    10381 aagcattttg agctaggtaa atttcacctt caccttcaac caccattatt ggcatttgtt
    10441 ctcctgtcaa tgacctctga acatgaccta ataatgctct tcccaatcca ccgccattac
    10501 ttggacctaa cagcaatttt tcaaaattaa aatttccttt aaaggctacc atagcacctt
    10561 tttttgcaaa aaatttacca cttccccttg ctatacacat aagctcgttt gtaacatcaa
    10621 atttaaacat aatattcact tatattgctt aatttagcaa taatccctcc taatcatata
    10681 tttatatata attatacagt aaaattacag catagtctaa cctacttcaa atatgaaatt
    10741 tgttatttat ataaaaaata agaacctgat aataaagcaa tttaactttt tatcaggctc
    10801 ttatcctatt tatctcttag cgctatgaca actcttacta cttgagcatc cagaacaacc
    10861 acatccacaa ctgctaccat tcttttttga atttttaacc atatacatta cagctagtgc
    10921 cattaatact actacaatag ctgctataat aaatgtagcc atattcatca ctccttattt
    10981 atatgaatta atttttcata taacttaatt tacccatttt ttcttttttc atatttttat
    11041 ttgaataagt taaaactaca aagactactg ctactgctat aaatatagat attattgccc
    11101 cagcaatact tcctccagta cctaaaacta aacttccaac ttgatttata agaagagcaa
    11161 ttatatacgc tgttaaagtt tggaatccta atgtaatcca tgtccatttc catgaaccca
    11221 tttctctttt tatggctcca atagctgcaa aacaaggtgc acaaagcata ttaaatgcca
    11281 taaatgcaaa cgcactagct actgtaaaca tgcttgcaac tgaagcaaga agtgttgggt
    11341 cttgctctgt tgcatctgat ataccaaata aaactccaaa tgttccaact acattttcct
    11401 tagctactag acctgtaact gttgctactg atgattgcca atttccaaat ccaagaggag
    11461 caaatattgg tgcaactata tttcctaagc tagctaatat actatctcca gcatctacca
    11521 tttgtaatga ccaattaaat gattgtaaga accagattac tccacaagca acaaatataa
    11581 ttgttcctgc tttgattata aatgcttttc ctctatccca catatgtatt aaaacacctt
    11641 ttgcacttgg aatatgatat tgaggtaatt ccataacaaa tggagatggt tctcctgcaa
    11701 ataaactagt tttctttaat ataattccac aaatgattat cattgcaata cctaagaaat
    11761 acattgatgg agctacccat gaagcacctc caaataatgc ccctgcaaat aatgctatta
    11821 ttggaatctt agctccacat ggaatgaatg tagttaacat aatagtcatt tttctatctc
    11881 tatcattctc tatagtacga gttgacataa ctcctggaac tccacaacct gaacttataa
    11941 gcattggtat aaatgatttt cctgaaagac caaacttacg gaaaattcta tccattataa
    12001 aagctacacg tgacatgtac ccacaatctt ctaatattga taataataag aataaaagca
    12061 tgatttgtgg tacaaatcct agtactgctc caactccacc aattagacca tcaactacaa
    12121 gtccttgtaa ccactctgct acatttaaag aagctagaaa attacttaca ttgccttgaa
    12181 taatttctcc aaacagtaca tcatttgtcc aatcagttgc tatagtaccc aatgaactta
    12241 ctgctatata gtaaactccc cacattataa gtgcaaaaat aggtaatgct agtatacgat
    12301 ttgttacaat cttatctatc ttatctgata cagtttcctt accttttcga ttcttcttta
    12361 ttatacttga aattattgaa ctaataaatt cataacgatt ggctgttatt atactttcac
    12421 tatcatcatc caattcatct tcacaatttc tagttatttc ttcaatacta ttcaatattg
    12481 ttttagaaat atcaagtttt tgtataacat tttcatctct ctcaaatagc tttatagcta
    12541 accagcgtgt ttctacatct atatatggag tcttttcttc tattattttt tcaatttttt
    12601 ctattgcatc cttactttca tcaacaaagg gtaatttgaa atttaattta ttgttactac
    12661 ttgcaagttc aactgccttt tcagcagcct ccataattcc ttgaccttta actgctgtta
    12721 cttctaccac tgggcaacct ataacttcag atagttcttt aatatttatc ttatcaccat
    12781 tcttatttac aatatccatc atattaagag ctataacggt cggtatacct agctctaaaa
    12841 cttgagttgt aagatataaa tttctctcta tatttgatgc atctacaata tttattactg
    12901 catccggctt atcatctagc ataaaattac gtgtaactac ttcttctaat gtatatggag
    12961 ataaagaata aatacctggt aaatctacta tttccacatc tttatttcct tttaattttc
    13021 cacctttttt ttcaactgtt actcctggcc agttaccaac atattgtgat gagcctgtaa
    13081 gcccattgaa cattgttgtc ttaccacagt taggatttcc tatgagacct atttttattg
    13141 acatttccat tccctccatt attatttaaa tacatcactg tttgtgatat agaaaatcat
    13201 tctcattata taattacatt aaccctactc taattaatct ttattattcc acaataattt
    13261 tttctgcatc tgacttacga attgatagtt cataatctct gactgttacc tcaattgggt
    13321 caccaagagg agctactttt cttataaata tattaacacc tcttgtaatc cccatatcca
    13381 ttatacgtct tcgtgtagct ccttctcctt ctaacttttt gacttttaca gtctctccac
    13441 acttaatatc cttcagtctg ttcatgtcat tacctcccta tattattatt ctacttgcca
    13501 tttttttatc taatgcaact ctagtatctt ttacgtctat tattaagtta ccatctatct
    13561 tagaaactat tttaacattt gtacccacta caaaacctat actatttaaa aaagtcctcg
    13621 tcttatcact tcccgaaatt ttctttataa ctacttcttc acctgtattt gataatatca
    13681 atggcatcat ataatatccc tcctcttaaa gtaatttaaa tctacaagca atcgttatct
    13741 tatatataaa ttacaatacc ttaaatctta atctattaaa ataatatcct attttttcct
    13801 tctttttaca attcctaatt ttgatttaag tctatcacaa tagtatattt tttgcaagtg
    13861 attattgtat tcttttgatt ttttttctca tttgatttat tttattaata aaatcgtaaa
    13921 aattaataca aaaataaaga tgctctaaaa tagtatactc aactaatatt aaagcacctt
    13981 tattatatat gtcattattt acacattgct ttattctttt ttaaaagcga ttttttcaaa
    14041 ctcttttaca ggcattggct tagaaaacaa atatccttgt accatatcac acccaatttg
    14101 ctttagaaat tctacttgcg aaatagtctc tacaccttct gatattactt tcattttcag
    14161 ttcctttgcc attttaatga cattagatat aacaattttt tcattattac tctcatcttt
    14221 ttctgtaaaa aatgctctat ctagttttaa tatgtcaaca ggcaattctt ttaaaagatt
    14281 aagagaggaa tatcctgtac caaaatcgtc catagagatt acaaatccct tttctttcaa
    14341 attgttcatt gtatcaagta atccttctat attatctaat actgctgttt ctgttagctc
    14401 taattctata agtcttgtcg gaattttgta tttatctaca attaatttaa actcctctat
    14461 aaaattacta tttacaaaat gcattcgaga caagtttaca gatataggta ctactttttg
    14521 tccttcatcc atccattctc taatcttttt acacacttcc tcaaatacat agaaatcaat
    14581 atttacaata aaaccattcc tctcaaatac aggaataaat tctattggtg gtattagacc
    14641 tttttgtgga ttatcccaac gaacaagagc ctctgcccct ataatttgat aatctgaaag
    14701 gctatattta ggttgaaaat aaacaataaa ttctccatta ttaagagcat ctaccattga
    14761 attttcaatt tctttctctt ttaatatttt tttgtgcatt tccttatcgt aaaaagcaaa
    14821 tgaattttta tgaccacctt ttatagtttt tcttgcagta ttagctctat ccataatagt
    14881 atttatgtca ttatcacttt tacttatttt atatatacca caatctaaaa tcaatttata
    14941 atagtttatt ttaggattac taagtatttg aaccttattg taaatattgt ttaaccttgt
    15001 tttaatatca tcatctgtct tatacttaat aagaagaaca aaatggtcag cagaaactct
    15061 tgcaaaaatt tcatcttcct ttaattcact tgcaatagta tttgatatat gtatcaaagt
    15121 atcatttccc atatcatatc caaacatatc atttatatat ttaaatttgt caacatctat
    15181 ataaaacaac gcatattctt ctggattatt tttagcaaac agtttattag aatttatttt
    15241 aaatttatct atactatctg ctcctgtcaa tgggtctgta tatgctatct tccttagata
    15301 atcctctttc ttctttttat tataaattat atatttaata gcaattaaga ataaaataca
    15361 tacaaaagaa ataatttgta ttgaatattc tttaaaaata acaccaaatg gtactttata
    15421 aggtttgtca atagtattag aatatataca atcacttaat tcttcttcag taattttgtc
    15481 tattgattta tttaaaatag acactaatat agggtcagtt ttatttgata cccctatacg
    15541 catcccatag cttgtatcta aaacagatat aacagataaa ttctcactct ttccttctct
    15601 taaaagttca tcaaactgat aagaactaag tatcattaag tttgctttac cttcatttat
    15661 agcatttaga caactttcta cactcttata agatgttgtc ttagcatttt caaaactctt
    15721 ttctatataa gaatcaatag acttataact actagttaaa gcaatattta aatcattatt
    15781 gagattggta tccaattttc ctaccataat cattggaata tttatgtatg gatttgtcaa
    15841 tttcatatta aatcgttttg ccttatcatt ttcattgatt gagccacata gcatagttgc
    15901 cttaccactt tttatgtaat caagactctc attataattt tttgttttaa tatattcaaa
    15961 ttttatacca catttttttg atataattga cattaaatcg gaacttatac ctttaaatga
    16021 attgctttct ttatcataat actctacagg agaccactct gaatcatata ccatagtaag
    16081 ttttgggttt gctttaataa aattcatttc ttgccgagta aatgctatgg aattgttgac
    16141 ctctcttttg aaatattttt tatataattc caactcataa tacatatcat ttatttttaa
    16201 ttcttttaaa gcataatcta attcttttac caaattaggt ttatcttttg cagtagcaaa
    16261 ataaaaaggt tctactgaaa atttgctaac tattttcatt cctttagcat ttataatact
    16321 tccacaaaca aatgcatctg cctttttttc attgacggac tcaattaatt catcaataga
    16381 attacagtat atatattcaa ctgaaaaatt attttcttct ctatacttct caaatgcagt
    16441 attatgaaag tttcctctaa gtacagctac tctcattccc ttaaaggaac ttatatcttc
    16501 ataaaacata ttattgttac tatcctttgt atatagtgca ctatattcat atcctgaact
    16561 tagtttagga aaattaaata gtttatttct ttcatcattc ttctgtaaag gtccgagtaa
    16621 gtctatttca cccttttcta gtctgtctaa acattcttcc caagaccctt ttacaaattc
    16681 atattcccat cttgtatatt ttgagatttc tcttagataa tcataaccat atcctgtata
    16741 agaatgctct tttgatgagg atattattcc atagtcatca caatatccaa ctcttatttt
    16801 ttttgaattt tcagactcat cagcaaagca tagtatattg ttaaaacatg atataataag
    16861 taaaaatatc aaagaaagta tcaagaagtg ctttttaaat gctttcatat ttaaaaacct
    16921 ccaatttgcc aacttacttt aacttatttg gttttctagt tgtaactact tttattgtaa
    16981 tggtaaatat atcaaattgc aatattctca tataatattc ttattcaact ataataaatt
    17041 tattattatc tatttaaata cactatctaa tattagtttc ttataagtca taaaagcaca
    17101 actaacacta taattctagt gattaatttt atttcaaaaa atataataac actactaata
    17161 ttttaaatat caatagtgtc ataaaaactt ttcagattaa ttaaatcttt ctatatattt
    17221 tataataatc taaatcatgc taaacattta gacagaaaac ttttttcttt aatagtaaat
    17281 acttttgttt gaatatcaga ataattattt gaatatctat ctataaaacc atgatttgca
    17341 tctaaaatct caatttggat attttgtttc ctagaaagtt ggcaagaaat agaatcaaca
    17401 tcaaatgaat cgtgctttgc aaatactaat aaggtaggac attttggaat tacatccatg
    17461 taatctcgaa tccgagagcc ataacaacaa attatgccat cacaaaaaga atcttcactg
    17521 catctccatg caatagtcgc tcccacgcta tacccagcaa caaatacata tttatactct
    17581 gattttaaat catttataag cttttcaact tttttataaa tatcaaaacc aacattattt
    17641 ataaaaaaat catatgcctc ttggactttt gaatatggaa aaactctttt ttctcccaga
    17701 agttcaggac aaaatacatc atatccatat ttataatact tttgacaaac atcttctata
    17761 aagctattaa tcccatagat ttcatgcaaa acaacaatag ctacatcact tttatgtata
    17821 tactttatca gattatccac cacctttaca aaacagcaaa ttttttatac ttagtttaat
    17881 atattcttct ttacatgtca atttactttt ctaaaatcaa atttattttt ggattaatca
    17941 tttattttta aaagttgtgc attattacta ttaaattatt ctctattatt aaaaatataa
    18001 tcgttagaaa taaaaaataa agtacaaata attaaaaaat atctcactca taactttaaa
    18061 aattataagt gagatactcc taaaatctac tttatattaa atttattttc caaaatatct
    18121 atttaataat cctaagaatg ctttaccatg tctagcttca tctttacaca tttcatgtac
    18181 tgtatcatgt atagcatcta atcctaattc ttttgctttc ttagctaatt ttaattttcc
    18241 atcagttgca ccatactcag catcaactct aactcttaag ttttcttttg tatctgcaac
    18301 tacaacttct ccaagaagtt ctgcaaattt agcagcatgt tcagcttctt caagagctat
    18361 tctcttataa gcttctccta cttctggata accttctcta tcagcttgtc tactcattgc
    18421 taaatacatt cctacttctg tacactcacc aacaaagtta gctcttaatc cttcgattat
    18481 ttcttcatct acaccttgag ctactcctat tctgtgctca tcagcccaat ttttttcgcc
    18541 tttcatttct tcaaatttat cagctccaac tttacatact ggacattgtg ctggtgcagt
    18601 atctccttca tgtatatatc cacatactgt acaaacaaat ttcttcataa ttactttcct
    18661 cctaaatttt atatatcaaa ttaaattttt tatttattaa gttagtctat atatacccgc
    18721 accatatacc tttaaacaat ttttttaaaa gttttttcta aatacctatt ttccctgtgt
    18781 caataatatg tttctataat aatgtccctt taattttatc aattctttat gatttcctga
    18841 ttccacaatt ctaccatttt ctaaaactat tatattatca caatttgata tggtacttaa
    18901 tctatgagct ataattatac ttgtctttcc ataagttaaa ttatttattg cttcttgaat
    18961 tttagcttct gttgctatat ctatgttact tgtagcctca tccaaaataa gtattggagc
    19021 attttttaat actgctctag caatacttat taattgtttt tgaccttcac ttaaatcttc
    19081 acccctatcc gataatatag tactatatcc ttcacgcatt ttaactataa atttatgtgc
    19141 atttgctatt ttactagcat atataacttc ttcatcagta gcctccagat ttccatacct
    19201 tatattttcc ataactgtat cattgaatat ataaccttct tgtggaacta ctccaaaaca
    19261 agttctcaaa tagtttctgt taatagagta tatatcctta ccatctaaaa atatacttcc
    19321 ttcatctata tgataaaaat ttgtaattag atttataata gttgtttttc cacttcctgt
    19381 ctctcctatg atggcatttg atgtgccagc tttgattttt aggtttatat tatctaaaat
    19441 tttctttttt gaatcataag aaaaacttac atctttaagc tcaatttctc cctttacaaa
    19501 atgagagttt aaattaccat ctttattatc gttaatctca cttttaaaca tgttatgcac
    19561 ttcttgctct tcttgttcat ctataatttc aaaaactctt tcacatccag ctatggctga
    19621 aagtaatgta ttaaatagat ttgctaattc atttaaaggt cttgtaaatt gtcttacata
    19681 acttaaaaaa ctagctataa cccctatggt tataatatct ttagatgcaa gaatgcctcc
    19741 tataatacat atagttgcaa atcctatatt actaatcaca ttcataatag gcattaaaag
    19801 agaagtgtaa atttgcgatt taagggaaat atttagaagt ctatcattga cttctttaaa
    19861 atcatttaca gattttcttt cataattaaa agattttact acatttatgt ttgatatcat
    19921 ttcttcaata tgtgcattta gttttcccaa ctcaacttgt tgttgcttaa aaaaaactct
    19981 agttctcttt gcaataaaat tactcacaaa cattactatc ggtattaata ttacactgca
    20041 aaaggttaaa attgggctta atacaaacat cataataaaa gtcatcaata tgtttaagat
    20101 tcctgtaatt aactgtacta atgaatttga tattcctgta ctgatattat caacatcatt
    20161 tgtcactcta ctcataatat ctccagtaga acttttatca aaaaaggtta ttggtagatt
    20221 tttaaatttt aaaaataaat tacttctcat ttctctgact atactttgag ataatctagc
    20281 tgtacatatc cccttagaca gtttaactga aaactcaatt atataaaatg aaagtaaaat
    20341 aattattcct tttaagaaaa tggattttga ataagtatcg atatctatta tgtcaactac
    20401 ctttccaata gtatatggta ttacagttga tataattgaa tcaattatta ctagcaaaaa
    20461 aataaatcta gtaatcttct tatctttttt aaagtacata aaaattcttt taaacgtttt
    20521 tttaaaatct ttaggctttt cttttttctt taatctactg accttctgat ttccagtact
    20581 taaattcaat atacttcctc cttaatttac atcttcactt actaaatttt gcaacgcaaa
    20641 aattttttta taaattggag aactaaccag taaatcttca tgtgtagcaa atccaaccac
    20701 ctcaccctca tccaagacta aaatcttatc acagtcaatt atagaactaa tcttttgtga
    20761 aatagttatt acagtacatt tcttactttc cttttctctt aaattactta tatactcttt
    20821 tatattcttt cttataattg tttctgtatt aatatccatt gcactaaaac aatcatcaag
    20881 aatcaatatt tgtggttttt tggctatagc tctagctata gtaattcttt gtctctgtcc
    20941 tcctgataaa tttttacccc tttggaataa aacttcatca taagtatttt cgagttttgt
    21001 tataaattga tttgcatttg ctatttcaca tgattttaca acatcctctt tgcatatatc
    21061 tctatttccc ataactacat tctccaatat actttttcca aacacactac tattttgaaa
    21121 tactacacct attcttttcc ttaaactttc ttcagaaata ctgtatatac tacgtcctcc
    21181 aatcttaata tcacctttat caattatata taatttttga attaaattta ctaaagaagt
    21241 ctttcctgaa ccagtttgac caatcacacc aatattttga ccttcttcta ttttaaagga
    21301 tacgtttttt aatatttttt catctgtatt tttgttatat gaaaaatcta cattttcaaa
    21361 aattatatca caatttacaa actttaaatc tttttctcca acatctaaac tttcttctct
    21421 ctcaattact tcttcattct catcaaccaa aacctcactt attctctcat aagatgcttt
    21481 tgccctaaca aaaacattaa aaacatgtga aatcattata agagatgtta atattctagt
    21541 catatagttt acataagcaa ctataatccc aattttaatt tctcctgaat aaattaaatg
    21601 ccttccaatc agtaatatta ttattatgga aacattaatg attattgtaa ttgtcggtct
    21661 aaaaaatgac atcatagttg tagcttttct actattttta aaaagttcat catttaactc
    21721 ttcaaagctt tggttctcat aatcgtatct acaaaatagt ttaactactt ttattccact
    21781 taaaaactgt cttaattttg aatttacttt atctgtactt tcttgtacat ttttaaataa
    21841 tctataccct atcttcatat taagcaatat tataataaat ataattaaaa ctgcaattag
    21901 aataattatt gacatcttta aatttaattt tatagctata attaaacttc ctatacacat
    21961 tattggtgac tttataaaaa ctctcatcat tccattaacg aattgttgca tttgattgat
    22021 atcattagta attcttgtta ttaatgaagc tctaccaaat ttatctattt gttttaatga
    22081 gtatgtattt atcttttcaa atacatcctg tcttaaatca taagaaaaat gctgagaaac
    22141 tcttgccgag attacacttc ttattattgc aaatattgca cctaaaaaag ttatacctat
    22201 cattatagct cctgttgata gtatgtagtc taagttctta tttgaaaccc ctatatctat
    22261 tatttttgcc attatagttg gttgagctaa atcacaaaaa gcttccaatg ttaaaaaaaa
    22321 tactgctgtt agaaataata ctatatattt ttttatgtat tttttaaaat acctcattca
    22381 atcatccctt catagaattt tctcattacc aaaagtatat cataatggct aatttaattg
    22441 attatatcaa ttataaatat ttgatttatt attaattttt ataccatata ttttataaat
    22501 tatttgtttt tatttcctat atttatatat atttatagca tactat
//
LOCUS       DAEMIK010000060        22483 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEMIK010000060 DAEMIK010000000
VERSION     DAEMIK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.060"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1396
                     /locus_tag="KPK87_001912"
     CDS             <1..1396
                     /locus_tag="KPK87_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG2538063.1"
                     /translation="STEPLIEFISDTTYKNKRSSYIKEVYFTRYIGVDDSIDKYIEKI
                     KNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSKFNSWDLYNIDFPCK
                     IENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWMDYFLPKLFYNKVKT
                     TISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYPECSKFSTLFELSRK
                     TPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLTSKKIVQDEEVELSY
                     EDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVVNDGYSFLVRFENDD
                     KVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIVAIGSPLGLFNTVSD
                     GIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAGYDDGQNLNLAVESS
                     LVKIFANNFIEIVN"
     gene            complement(1488..3362)
                     /gene="asnB"
                     /locus_tag="KPK87_001913"
     CDS             complement(1488..3362)
                     /gene="asnB"
                     /locus_tag="KPK87_001913"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG2538064.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3565..4239
                     /locus_tag="KPK87_001914"
     CDS             3565..4239
                     /locus_tag="KPK87_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG2538065.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4352..4888
                     /locus_tag="KPK87_001915"
     CDS             4352..4888
                     /locus_tag="KPK87_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2538066.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5054..5674)
                     /locus_tag="KPK87_001916"
     CDS             complement(5054..5674)
                     /locus_tag="KPK87_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG2538067.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(5988..8126)
                     /gene="vanT"
                     /locus_tag="KPK87_001917"
     CDS             complement(5988..8126)
                     /gene="vanT"
                     /locus_tag="KPK87_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG2538068.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8144..8950)
                     /locus_tag="KPK87_001918"
     CDS             complement(8144..8950)
                     /locus_tag="KPK87_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2538069.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(8947..10047)
                     /locus_tag="KPK87_001919"
     CDS             complement(8947..10047)
                     /locus_tag="KPK87_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG-Cd"
                     /protein_id="HBG2538070.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLISDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10308..11390)
                     /locus_tag="KPK87_001920"
     CDS             complement(10308..11390)
                     /locus_tag="KPK87_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2538071.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(11440..12141)
                     /locus_tag="KPK87_001921"
     CDS             complement(11440..12141)
                     /locus_tag="KPK87_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG2538072.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12575..15106)
                     /locus_tag="KPK87_001922"
     CDS             complement(12575..15106)
                     /locus_tag="KPK87_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2538073.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15214..15831)
                     /locus_tag="KPK87_001923"
     CDS             complement(15214..15831)
                     /locus_tag="KPK87_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG2538074.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTTDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIIKPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16193..17077)
                     /locus_tag="KPK87_001924"
     CDS             complement(16193..17077)
                     /locus_tag="KPK87_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG2538075.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17268..18518)
                     /locus_tag="KPK87_001925"
     CDS             complement(17268..18518)
                     /locus_tag="KPK87_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2538076.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(18740..19504)
                     /locus_tag="KPK87_001926"
     CDS             complement(18740..19504)
                     /locus_tag="KPK87_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538077.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNENTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19498..20223)
                     /locus_tag="KPK87_001927"
     CDS             complement(19498..20223)
                     /locus_tag="KPK87_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538078.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20225..20593)
                     /locus_tag="KPK87_001928"
     CDS             complement(20225..20593)
                     /locus_tag="KPK87_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2538079.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKITLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(20769..22313)
                     /locus_tag="KPK87_001929"
     CDS             complement(20769..22313)
                     /locus_tag="KPK87_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2538080.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLIRG"
BASE COUNT         7744 a         3796 c         2351 g         8592 t
ORIGIN      
        1 gtcaactgaa cctctaattg aatttatatc tgatactacc tataaaaata agcgttcttc
       61 atatattaaa gaagtctact ttacaagata tataggtgtt gatgattcaa ttgataaata
      121 catagaaaaa attaaaaata tagatactta tttttttaat aatcctacaa ttccctatat
      181 tagattaaac aatctaaaaa taaattttga caaagaacaa acagataaga tgcttaaaat
      241 ttttaatatt tataatagtt ctaagtttaa cagttgggat ttatataata tcgactttcc
      301 atgtaaaata gaaaatgaca ccttaaattg gactaaaaag attgcattta aaaatacttt
      361 agatttattt tccacaagta tgccaaattg tacatcctct atcatcaaaa actttgcagt
      421 taaacttttg tgttggatgg actattttct acctaaatta ttttataaca aggtgaagac
      481 tactatttca ccaaagatag tatactttgg aaatattaaa aggcaagaac tttttttctt
      541 atatttttta tcacaacttg ggtgtgatat tttatatata aatccaaata aagatattct
      601 tgatttatat cctgaatgtt caaaattttc tactctattt gaactttcta ggaagactcc
      661 aaatatatta gaaatacctt ttgaaaggca tattacaaat ataaattaca gtgaaaatac
      721 caataagact tctcctacta cgaataaacc taactctaaa aatacactta ataacacaaa
      781 tctaacatct aaaaaaattg tacaagatga ggaagtagaa ctttcttatg aagaccttgc
      841 aaagttatca gcttctgttg ttatgattgt tgtgtgcaat aacgaaaata aaccgtttaa
      901 gagcggttct ggagtagtaa taaataatga aggttatata cttacgaatc ttcatgtagt
      961 caatgatggg tattcatttt tagttagatt tgaaaatgat gataaagttt atacatctta
     1021 ccaaattata aaataccatt cagattatga tttagctgtg ataaaggttg atagaaaatg
     1081 caagccaata cctgttaaag tcagtaaaaa acctgtgaga ggtcaaaaaa tagttgcaat
     1141 tggaagccct ttaggtcttt ttaatacagt ttcagatgga attatttcag cttttcgtga
     1201 ttttgaaacg gttcaaatga tacaatttac agctccaatc tctagtggta gttcaggagg
     1261 agctctttta gatatgtttg gaaacttact aggattgatt tccgctggat atgatgatgg
     1321 acaaaactta aatttagctg ttgaaagtag tcttgtcaaa atatttgcca ataattttat
     1381 tgagatagta aactaatcaa tcaaaattct acttatccta ttccataata atcaagaggg
     1441 tgaaaacatt gcatgttttt gccctctgat taatattgaa aacagtatta atactcgaca
     1501 tttttatgct caataaataa ttgatgccat agacaaaatg taaatatagt ccatattttt
     1561 ctcgaattat ctctatgacc tttaagatgc gtatctaaaa gttttattgc atatttttta
     1621 tctataaatt catctacatt tgaattataa atagtttcct ttacaatatc ccctagagaa
     1681 tcttttaacc aaactcttat tggagttgga aatcctaatt ttttcctttg aacaacatgt
     1741 tctgggacta tttctctaaa cgcctctcta agtaaaactt ttgtattatt cttatttatt
     1801 ttttgctcca ttcttaaatt ttttgctaca tcaaatacat ctttatataa aaatggtact
     1861 ctaagttcaa cctgctcagc tgtagaaagt ttgctaattt tttgaagtat atcgccttga
     1921 agccatgtat tcacatctat atgttgcatt ttacttatat aatcgtaatt atttttttgt
     1981 gcatctctat acaaattaga caacaggtat tcatatatat tcttttcctt atacttaaaa
     2041 aagaatcttt ttacttcgct gttttcaaaa acttttgcat ttcctatata tcttttttct
     2101 aatggtgttg tagctctata aagataattt cttcctttta tgtttggcat aagttctgaa
     2161 actcttccta atatactttt aatataagta ggtgaattga caactgattt catagtacta
     2221 tactctttat atatattata tcctccaaat aattcatctg ctccttctcc agacaatgct
     2281 actttaacat gtttacctgc ctctttaatt aagaaataaa gacctacttc tgaagggtct
     2341 gctatagggt catccaaaga ataaattaca ttcggtaatg attttatata ttcatcttgt
     2401 gttatattaa tctggatatt ttctatacct aacttatcag ctgttttctt tgctacctct
     2461 aattcactat atcctttgac actaaagcct gcagaaaaag attttatatt tggattaatt
     2521 tgtgaagcca cagtagttat aatagatgaa tctattccac cagacagaaa tgttccaatt
     2581 tctttctcct cttccatatg agttactatt gaatctacta ctatatttct tatatcatcc
     2641 tcgttcacat ctctattgcc taagtgattt ttagtataaa aaaattttgt actacttcta
     2701 aactcaagtt tattatatct ctttaaagat agtataccat tttctaccct aaaatagtga
     2761 cctgctggta tcagccttat tccttgtatc atagtatctt caggaagtac atattgaaat
     2821 gaaaaataac tttgtaagct tctttcattt atatttaaat ttttcatata ttcaagtaaa
     2881 aacttatact ctgaggaaaa aattattgca ttttccttat ttatataata aagtggtttt
     2941 atcccaaata aatctcttgc tccaaatata ctttcatttt ctctatcata gattataaag
     3001 ttgaacattc ctttaatctt atgtacacaa ttcttaccaa attctatata agatgccaat
     3061 aagacttcaa tatcatcatt tgtagtaaaa ttgtgtcctt ttttaattaa ttcttctttt
     3121 atttcatgat agttatatat atttccatta aaaacaataa cttttccatc tttttcaaat
     3181 ggtttatttc cataaatagc atcaattaca ttaaatcttt caaaatcaaa agttttttcc
     3241 tcatccttat actctattct actagaaagt cttgtattat ttacagattc ttttatttta
     3301 aatttaatat cttctttttt agatatatca cctttataat aaattgaaat attgccgtcc
     3361 atataatcaa aattcctttc tgtcctttag tcttattcta tatttcatag ttttgtcttt
     3421 ggctttaaaa cttattcaat ttaaaatgaa attgctatta aattcttata aattatctgt
     3481 taatatttca tatttttttt atttctagaa atactatatt aggaggtgat ttttatgaag
     3541 aaaaaaatat taataccagt tattatgtct ttatttataa tctcacagtg cataacttca
     3601 tttgctttta cacctgaaaa taacaaattt aaggttaaac cattacctta tgcatatgat
     3661 gcacttgaac cttatataga taaagaaaca atgaaactgc atcatgataa gcattatcaa
     3721 gcttatgttg ataaattaaa tgctgctctt gaaaaatatc ctgagcttta taattattct
     3781 ttatgtgaat tattgcaaaa tttagattct ttacctaaag atattgctac aactgtaaga
     3841 aataatgcag gtggagctta taatcataaa ttcttttttg atataatgac gccagaaaaa
     3901 accatacctt ctgaatcttt aaaagaagct attgatagag actttggttc ttttgaaaaa
     3961 tttaagcaag agttccaaaa atctgcttta gatgtctttg gttctggttg ggcttggctt
     4021 gtagctacta aagatgggaa attatctatt atgactactc caaatcagga tagccctgta
     4081 agtaaaaacc taactcctat aataggactt gatgtttggg agcatgctta ctatttaaaa
     4141 tatcaaaata gaagaaatga atacattgac aactggttta atgtagtaaa ttggaatggt
     4201 gctttagaaa attacaaaaa tttaaaatct caagattaat taaatattaa aatccttttg
     4261 aaaaaaactt atgtttgcta tataatataa taaaagatag tctaaattta ccaatataat
     4321 gtttatagtg acgattcagg aggattttta tatgataaaa gaaacacatg aaaggggagg
     4381 ttatatattc gcacttttga tactcccctt tataaacaat ctatacctag ttaaatacga
     4441 tattgcatat aaaatagtat taattataat ttatatttat tttgcatatt taggttcttt
     4501 atttccagat atagatatga gaggttctta cataagtaaa aaatttatgt taatatacaa
     4561 attatttgga agtaggttta gacatagagg gtttactcac agtctaattg ccttattact
     4621 tatttatagt ttttttaaat ctctgacaat cttcactaac aataatatag ttttctcatg
     4681 tttgtccagt gggtttataa taggatatct atctcacttg tgtcttgacc ttattactaa
     4741 agaaggtatt gaattgtttt atcccattac cataaacatt tctttactac ctataaaaac
     4801 taactcaaaa acagaaaaat tcatatctaa gctattaaat ttcatagtta tttttttgat
     4861 tggatatcaa ttttatattt tattatagtt gtagaaaaaa taaataacaa tattttgaac
     4921 taagtataaa taagttttat atattttagt ttacaaaaga actaaaaatt ttgtacctta
     4981 atacaaaaaa taaaacgtat cttaaaataa tttagtacta aatcatttta agatacgttt
     5041 tatttcgtac aagctaaatc attttatcat ctttatcatt gtgatgtttt ttatgatgga
     5101 aatcataatt atgattttca agatttttat ttactctatt tccaatcttc catgacaaaa
     5161 acaccaataa tataaatgat gctatcttta aaggacttgt tataaaacct tctatatcac
     5221 ttgccacgta acttataact ataaacatta caaattttcc tgccaacatt gctggcacaa
     5281 aatcttttaa atctttttta ccaaaagctg atgcgatagt aactaaacaa ccgggcataa
     5341 atggacaggc atacgctata aataataact taaatccctg tttatccatc caacttattg
     5401 ctttttcagt tttactattt ctcaacttat taaaaagctt actatcatta aatctgctta
     5461 ttaataagaa taatgaagtt gtcccaagtc ctgagccaat ccatgataac aaacatccaa
     5521 taaataatcc ttgtattgca gcatttgctg taactatagc aacaagtggt aaggctggta
     5581 taaagctttc tataaatgca cttaataagc cgattattgt ggctaacacc caataatttt
     5641 gagtcaaatc aagtagttct tgtatatact gcatattgct tttccccttt tttaacttta
     5701 actattatta tatactctat atgtttatta tataccaatc tcaaaaaatg aatctaatat
     5761 taaaaagtaa ataatctata taagtttaca gttaaggtat aattatttta ttatatatta
     5821 taacaaaaaa ggtgatgttt caaaataaaa atctgaaaca tcaccttttt ataacttaaa
     5881 ttgtcatgta agttataaaa aatatatatt agacataata ttactaaaaa tgtctagtca
     5941 gagtaaaata aattacacag tacaacaaat attactattt aattgagtta cttctttata
     6001 acatttaacc ttcttcctac tctgctcaat aattcattcg atatactaca tgagttattt
     6061 gcaacttcag aagcagataa ttcagataaa ttatctcttc ctattattat tgccgtatta
     6121 ccaacttcta cattaggtat atctgtaata tcaacagcaa gttggtccat gcaaattcga
     6181 cccactattg ctgctctaca accatttatt attacgtaac tttttccaca ggataaattt
     6241 ctaggtatac catcggcata gccaatagat aatatagcaa ttacgctatc tctgcttgca
     6301 ataaagactc ttccatatcc aaaactatca tctttttgta tttttctaat aagaattact
     6361 tgtgatttta aggataaaac aggtcgtaaa tcaagttgca atttagtcgt atcatttgga
     6421 gaacttaata tcccgtataa tgcaattcca attcttacat aattacactt aagattggga
     6481 taattaaaaa aaccataact actttgaata tgtactttag gaatttttat accttttttt
     6541 aacagaaaat caattaactt ataaaaacat tcaatttgtt tatttgtaaa gtctacatct
     6601 tcatctttca gactatctga aacacacaag tgtgtaaata tgccacatat atctaagtat
     6661 tttaatttaa atgcctttaa aattgacctc acatcattaa catcaaaacc cagtctgtgc
     6721 atacctgtat caattttaat gtgtgatttt atgttgtatt tctgtttgtt taaagagata
     6781 gcatgattaa ggtcaataac actctgtgta agattgtatt tatgaagttc ctttgccctt
     6841 attgggttag tatatccaag aatcaatatt tctcctagta ttccatatcg acgtaaatca
     6901 atcccctcat ctattgttgc tactgcaaat gcttttacac ctatttggtt tgcacaagtc
     6961 gctacttcaa aagcactatg accataggca ttagctttta caactgccat caattcacaa
     7021 tcttctggca tagctttttg tatcatcttt acattatgtt ccaaattatt taaatcaatt
     7081 tcaatccaag ctctattggt atttacctta tgttctcctt tcaagtcttt ttttagaggt
     7141 ttaagaatca atataataat tatagaagct ataaaagatg ctatacttac cactatataa
     7201 tgaataaggc tattgtcaac caagacagtt tgtattccga atattttaga aaacatacga
     7261 ataacaacaa tcatcatagg atgtataata taaataatta atgatgatgt tcgcaataac
     7321 ctagctcgag aacctttcaa aaatgttaag gctgtaaata agaaatacat acatggcaat
     7381 aacataatat acatgctatc atgacgctgt acccctagtt tatgtaaaaa cattccctca
     7441 cataacataa gtaagaatga tataagaaat ccaatcaagc tatttttagt tgacatataa
     7501 ttagatttat tagatattat tccaccaagt atgaaaaata caggtgcaaa aaatatacca
     7561 tttcgtgtat aatctgatac ttcaaaaata tacccatata aattctttaa tagtggtatt
     7621 ttttctaaaa aaccataata actatcacca aacataccaa ttacataaat aacaatagtg
     7681 attactagtg agtactttaa acctagtttt tttacaagaa agtatgcaat acttgcacca
     7741 ataattgatg caggaagata ccataaatga tataaagtcc catcaaatac aatgtcctta
     7801 ataatattgg gcactaaatt attcatggaa aaatatccag tgtaaatatt taagggaata
     7861 taaattacaa tagctatccc gtaaataaat aatacatttt ttagaaaaga tttcagttta
     7921 ttacaattat aattatatgt tgaaattaga aaaaagcctg atagcataaa aaagaatggc
     7981 acagcaatac gagcaattat actagttaac acaaagtcag ccattttact atatgataat
     8041 aatggagatg tatgaattgc aattataagt aatgcagcta taatcctaaa ataatcaagc
     8101 cctgcataag atttattcct aatcattctt gttcctccat ttcttagtcc tttctatttc
     8161 tccaaaatgt cacaataaaa ccatccttat tgttacccga tatttgatat acacattgtt
     8221 caggtataga gattctcact tcttctttag tatgaatagg tacataaaag atttctataa
     8281 ttttttcttt atcttctatt tttaaacaat ttgtatacga aaatctctta atatattcta
     8341 tatattcttc aagagcgaga ttcattttat tgataatctc agaatgcgga tatcccacat
     8401 aacgaaaatg ccatggttct tgagatattc ctgttatttc ttctttttta cactgataac
     8461 gctctataaa accataatgt agtgctgcct tacgaaactc attacaaata ccattataag
     8521 gaaattcagg gcagataaaa tctataactt ctttctttaa ccctaaatca atagcaagcc
     8581 ctgtctgatg ttcactgtga tttggaagtg ctacaaactt tttagtaaag tcttttccat
     8641 tgtcacgcag agaacttgaa tatatatctt gttgttcctc tgcagtacga tatccactta
     8701 caggtactat ttcatttatt ccacatatac tttttaaaat attttgtaaa atgtttgtag
     8761 cttcacgttt cataagaata cttggaaacc ttgtatctac aggaattaaa tctatatctt
     8821 cagatgtttt aactggtaaa aaagcattta ctagaattag attcccatca taaactttat
     8881 ctttagtcaa taagatagta tttgtcattt tatcttcttt atttttatct aataaaatat
     8941 tattcatcat tttatgtaca cctcaattat cttgtccaat atttcttcaa aagacagtcc
     9001 aatacctttc atcatatttg gataacgact atgagaagta aagccaggaa ttgtattgac
     9061 ttcattaaaa ataatttcac cagatggagt taaaaacata tctactctag caaatccaga
     9121 acatccaaga gttctataaa taatttttgc tgtctcttgt attcttttct cacaatccaa
     9181 atcaattcta gctggcatat gaatttttga acttttcaat gtatattttt ctgtataatc
     9241 aaaaaatcct tttgacaatt ctatttcatc tactcttcca acaagtaagt cattgtttcc
     9301 taaaactgca caccctacct caaaaccatc aatattttcc tcaattatta cttcattatc
     9361 atgttcaaaa gccaagtcta ctgctacttg taactcttct tttttataaa ttttactaat
     9421 accaaatgat gaacctgcac gcacaggttt tataaataat ggataagtta agtcttttga
     9481 cagttcagcc acattaaatt tatctgagta ttttagtaat attgattttg gtactttaat
     9541 accttctgaa tgaacaagtt tatgtgattt gtctttgtcc atacataatg ctgaagaaag
     9601 agtaccacat ccaataacct gtatacctgc caattcaaat aacccctgca ctgttccatc
     9661 ttctccattt tttccatgca atacaggaaa tacaaaatca agtttagtgt atttaatatc
     9721 attttcacat gattcaatag cactatttgg aatatgattc tgagaaacag catcagaaat
     9781 taaaaattca ataataccat ttttgacacg gttctgcgaa acaataactg gaattatatc
     9841 agtatccctt tcaaaccaag tgttattctg gatattatta taatctccaa tataatgata
     9901 ccaattaccc tcccttgtaa ttccaattgg tagaatatca tatttttccg tattcaaatt
     9961 ctcaagtact gcaaaagctg attgtaatga tacatcatat tctgttgagt ttccaccaaa
    10021 tataactgca atttttttat tattcatctt tgattctcct ttgttgattt atttattttt
    10081 atacaatcta cagaaacatg ttaaaatact ttttctattt caaatccaat caaccttcac
    10141 ttacaattgc atctgttcca cataatatat tttcacattt ttctattgat ataattactg
    10201 tatcaaaata aaagcactct tgttttgata cagtaattat atcaataaca agagtgctct
    10261 ttatttattt atcctaaaga atttcctatt tttttatact taaattctta caaatttcct
    10321 acaatcggaa taataacctt aaaggtaatt atattgttct cgctatgagc tgttattgtc
    10381 ccgttatgaa gctccactat ttctttagct atagcaagtc ccaacccagc tctaccactg
    10441 tttgaattac gagatgtatc caacctgtaa aattgttcaa aaatacgctc aagtttttcc
    10501 tctggaatcg tattgctatg attcataaat ttaagcttta tataatcatc ctcctgtaca
    10561 gctgttattt caatagtagt atcttcaaaa ctataataaa cagcatttcg taataagtta
    10621 tcaaatacac gttgcatttt attaacatca caccttatca tcatatttgg tgtaacattc
    10681 agtgtatatt ttaaattctt actcatcaaa gtaggtttaa actcatatgt tagttgttca
    10741 agcatacgag ttaagcttac tgtactgtat tcaagtgata tatttgataa attaaacctt
    10801 gtaatttcaa agaattcatt aattaaatct tcaagacgct ctgccttatc caacgaaata
    10861 gataaatatt tttttcgcag ttcttcagaa atctgacttt catctcgaag tagtgtcaga
    10921 taccctataa ctgatgtaag tggtgttttt aaatcatgtg caagataaac aataagttca
    10981 ttcttacgtt gttctgccat ttgtgtctca aatttacgtt gttcaagtat atgtttaatt
    11041 gagtttattt tcttttctat tgccaataac tctggagata aagccacttc tccaacatct
    11101 tctttgataa gagagtcaat accttgattt atttcactaa aatattttgt aaaccaattt
    11161 aaataaacac ggaatattat aaaaaacaca atcaccatag ctagtatcat gaagagctcc
    11221 atgctattac ggaaaatctg ctgatataaa ttcaatgcag ctttatattc catacgaaaa
    11281 gcattttcaa agaatgatac tatccagata gaaaacttac catacaatat aaaagaatat
    11341 aaaatccata tgaaaataat ggctataaat gccattaaag ctgttctcat aaaaatttta
    11401 tttctcactt gagtaaagtc aggattgact ttaatatttt tacttttcaa ttgtataacc
    11461 tactccccat acagttttta taaatttcgg gtttttagaa ggttcatgaa ttttttcacg
    11521 caatcgccct atatgagcca taactgtgtt attattatca agatattttt ctccccatac
    11581 agcttcaaac aattcttcag aaggaaccac ttttccttga tgttcacata aataccaaag
    11641 aatagaaaat tcaatcggtg ttaatgctac ttctttccca aataaactac atttatgtgt
    11701 ttctttattg ataatcaatc ctttaatatc atactcatta acaattatcg actgttgttc
    11761 ataagaatta ttatatcgca tatatcttct taattgtgtc tttactcttg caacaacttc
    11821 caaagggtta aatggctttg ttatataatc atcagcacct atggttaaac ccattatctt
    11881 gtctatatct tcaattttag ctgttaacat aatgatagga taatagtatt tttctcgaat
    11941 tttctggcaa atatgaaatc catcaatatc aggaagcata atatctaaaa tagccatatc
    12001 caaattttta gattcaatac attctagagc ttccttacct gtataaaact tgtatactgt
    12061 aaaaccttca ttttttaaat atacttcaat taaatctgct atttcttttt catcatccac
    12121 aatcaaaata ttttcgttca tagattattt ttcactctcc ttattatcga ggtcatcatt
    12181 tataatattg gtaaatttac atatcaaata attacaagca tatttgtaac taatttttat
    12241 aagaaaagaa ctactacatc agaatgtgtt gatgctacta ttggaacaca aaaactgaga
    12301 ttaattgtta ttataaaaac tcccctaaca cgtccttatt atcgcataac aggtattaac
    12361 tttcaataaa aatatctatc agtcaaacac aattcaattt attataccag agacaatcca
    12421 tataaaaaac aattaaacct atgtatgtaa ccacagacat tactaaaatt tccatgttat
    12481 tttatataaa aattcaaaaa aggagttgaa tttaaaattt gattagaaaa aaatcaattt
    12541 taagacaact cctttttatt ttaatgtatt taaattatag tatatcagcc ttcataatat
    12601 cctcaaaatc acctttttca gatattaatc ttatcaattt aacagtacag cttaaatcat
    12661 ttataagtat agctccaact aatgaaccat ctttcataaa taatttttta tatattcttt
    12721 gaccttcatc ttttgatgaa atttctttat caaaattttg tatattacca atactaaata
    12781 cttttacatt cattgcatct aatgaaactg gatatacttc actttcaaat gtcaaattat
    12841 ctccacttgc atttgcccct gctatttttc ccatttctac tgaagatggc catattgcta
    12901 aactaatatt tcccacttgt gcaacatctc cacaagcata tatatcttta acatttgtct
    12961 gtaatgtctt atctacaaca atacctctat caatttttac agatgtatcc tgaaccattt
    13021 gagtgtttgc tcttacacca attgaaaata ctaccatatc acagtcgaca ctatctccat
    13081 ctttaaatac aactttttga gcttttccat caccttcaat accatcaact gctacaccca
    13141 atcttacatc tattcctgta tctcttacac attgttctaa tattttacta ccttctttat
    13201 caagttgttt tgatagtatg ctatccatgg cttcaactac tactacttct aatcctttta
    13261 gtctaaattc ccatgcagct tcaagtccca ataaaccgcc accaatcact acaactttct
    13321 tagatttttc tagtgcttct tttacactat ataaatcttt tatatttcta agagtaaaca
    13381 cattttctaa atcatggccc ttaattggtg gtataaagtt tctacttcct gtagctatta
    13441 caagcttatc aaatttaata gctccatcat tcactataat agttttattc acttcatcta
    13501 atttatctac attcgttcct aatattacta ctatattatt tttatcatac caatctttat
    13561 cttccatgta gaaatctgaa ccaagttctt catttatacc atcagaaagt gcaggtctat
    13621 agtaagtcaa ctcatcttca ttagaaatca tagttatctt acaagtttta tttctttttc
    13681 ttatagcatc tgctgcataa aaaccagctg ctccatttcc tactattacg aaatattcat
    13741 cagtatcttt tctaaaagta acttcatctt cttcaacttc tataaattgg tcttccttag
    13801 ctccacatac agggcataca ctaggagcac tatcaccttc aaaaacttca ttacaaatta
    13861 cacatttaaa tttcttagtt ttctttttac ttccaaaagt agaatttaac ttatctagga
    13921 atttacctgt ataagaatgt aatttatcaa tctcttcatt tgatggttta aagttaacag
    13981 caaaaggttt tactgctgtc attctaagct gacttattct ctccatagca ttttctatac
    14041 cttctccact ccatccatat gaaccaaata ctgaagcaac ttttccacca tgaagaactg
    14101 ggtttaacga tattaatata tcccatatag gctttaacgc atctcctaat attgttggtg
    14161 ttccaaataa tacaccttgt gatactgcta tttttgccaa aatatcttct tgcttatgct
    14221 ctattacatc aaataaattt actttatagt tgctattttt ttcaatatat gcttttatag
    14281 cttctgccat aatctttgta tatccatgtg cagaaacata acaaattgta acttcttttt
    14341 ctaactttat ttgttcattt acactccagt tgtaataaag gtctataatt tttctaggat
    14401 tttcagttaa tactggacca tgacctggac atacagtatc tatatctaaa tcttttatct
    14461 tttctatagc agttaccata gatggtttaa atggacccat tatacaatca taatagtatc
    14521 ttaaagcatc caagtaatcc ttttcttcat tttcatcaag tgtatttact atcttatcat
    14581 tactatagtg actaccaaat gaatcacatg ttacaagagt tttatcctct tttatatatg
    14641 tgtatatggt atctggccaa tgaagcatag gtacagagaa aaattctaaa gttttgtccc
    14701 caattgataa tgtatctcca tctgtaactg caacatattc aaagtctttg tttacgattt
    14761 ccttaagata tttaattgct gtttctgaag ctactacttt tgcattttta gctaaatcca
    14821 aaagcttttc aacgcttcct gcatggtctg gttcagtatg acttacaaca atatagtcta
    14881 ttttttcaaa atctatattt aaatcattta atctttcaat ataattgtca aaatgcttat
    14941 ctttaactgt ctcaaataaa actgtttttt ctgttccttt taatacatat gaattataag
    15001 tagtcccata aggtgtatac ataattatgt caaatactct taaatcaggg tcaagtgaac
    15061 ctacccaata taaattttct tttattttta gtgatttcaa tgacataccg tactcctcct
    15121 aaaatagtta tttagtctct ttataccctg cttgttatat tttaaacaaa atttattcaa
    15181 aaaagaaatt attttaaaga tagtttaaat cttctaatct tctttttcta ccttcaatac
    15241 agttcccttt ttagaatcta cttttatgtc atattcattt ttatctttat ctattcttat
    15301 tttataaaaa gtaacatcta aatctttgtc taaactccat tctttaacca ttccattttg
    15361 ttcttttaat gcaatttcca tagcttgttg aggtttaatt atgtcattta aatctaattt
    15421 ttcttttcta gcaactccat ttaaatcttc tgaatccaac ttttctgttt tatcttctaa
    15481 tacatctttt gtattagcat caattttcat cttatattca ttattatcat ctacaccttc
    15541 cacagtataa acaaatttat ttaaatctct ctcaagactt aaatctacta tatcagcatc
    15601 cttatattta tctttaaata tgtttattgc atctgtgtat ggcactttaa aatcttgttc
    15661 cattaaattt tcattatttt tttcatcttt taaagcgtta tttgatgcat ttgcctcctt
    15721 ttttgtatta tcttgtgctt gtcctgaatc cgtagttgga ctcccactac atgcagttaa
    15781 taaacccgct ataagcatac ttaccactaa taaatacgtt tttttacaca taaaaaccct
    15841 ccttaaatat attaatttat tctatattat gccctctaaa ccctttattt acccttttat
    15901 attaaaaatt cctcctattt tatcaatttt aaaactttat atttactaaa attaaagtta
    15961 cataaaaatt atactgtctc aaaatagaaa caagcatctt tttcattgtt gaaataataa
    16021 aaatatattt tatttcgtac aaaaagagtg cctcatgaac taaaaagttc attttgagac
    16081 actcccattt ttttatattt ttacttacaa aataacagta cttattaatt ttttacaaac
    16141 cttataagta tatctatatg atattcttaa atttaaacac tatattaaaa aattaaattt
    16201 tgtttaattc ttttaccgtt aaatccattt taggatatgg aatcgtaatt ccttcttcgt
    16261 caaacctaac tttaacctct tctagtaaat caagataaat tgtccaataa tcttctgtct
    16321 tgcaccaaac tctagttgca aatttaatag cattatctcc atgttctact actcctataa
    16381 atggttctgg ttcatttatt attaatttat gattattaac aatatcttta agtactcttc
    16441 taacatgtag tatatcatct tcatacccta cactaaaagt caaatcaact cgtctagtat
    16501 tctttgctga atagtttatt agactatcat ttgatacaga accattaggt ataagtatct
    16561 gtttattatc tggagtaacc aattgtgtat aaaaaagtcc tatttgttca accgttcctc
    16621 catggcctgc tgcttctacg taatctccta ctttaaaagg tctaatcaat agtataataa
    16681 atcctccagc aaaatttgat agactacctt gtaaagccaa acctattgct acccctgctg
    16741 atgctatcac agcagcaaaa ctggacaaac tcattcccca atattccaga acaatgatta
    16801 taacggcaac tttaagtaca ctcaaaagaa gagactttaa aaactttctt aaagttaaat
    16861 caatatctct tttactaagt aataacataa catggtttac aaattttttt attattttaa
    16921 atccaataga aagtataagt atgcctatta taagttttac tccgcttgta gtagcccatt
    16981 ctaatagact ttccattata cctcccatat ccatcttatt aatatctaaa ttatttaaat
    17041 ctaacttgct tatatcatct attttcgtcg tatccataaa aaactcctta ccttatattt
    17101 tagaaccaat attatagttt aaagttctta ttttctattt aatataatac aataccattt
    17161 gtacatagta aacaagattt atattccaat tatacatatt aggaaaatgc aaactatttg
    17221 ttatatatat gcaaaatata atactaaatt aaactattaa taaaacacta tatagcctta
    17281 aaatcatcat tttttacccc tatatgagat gatactatag ttataatact tagtactata
    17341 aatactacaa aacctttttt atatccatca actatattat atgaatttac ttgtaaagag
    17401 atagctataa tcaaattaat aattccactt cctataaatt caaaagtatt aataacacta
    17461 gttgccagtc cagaataaat ttctttattt ttatacttgg catcattaaa aacctgtaga
    17521 tgtgacataa ctatacatcc cataataaag aatgatattg gtaaaactat aattggtggt
    17581 tttacctggt atacaactac aattacaatc catataaaca aatctatcat tgccccaaat
    17641 tttattatat taaatttgct agaccttatt ttagcaaata caaaatccat aattattgaa
    17701 ccaaatataa atccatatgt aaaaaatgat actataaacg cagaaaaact cttactcaca
    17761 ccataaacgt ccattatgta tcttactccc cacaagctta taaatgcact tgtaagacca
    17821 acaaaagaga acattatcat tgaattatac catgttgatt tgttctttat aacagactta
    17881 ataccatcaa caatattaac cttctctgac ttttcatagg gttcagtatc tacattgaat
    17941 ccatactctt ttggtgtatc tctaacaata atatacataa aacatccaat tacaacacca
    18001 attataccga ttaataaaaa tgaatttctc caaccaacaa gttcagacaa aaacacaagt
    18061 ggaaaagtag caagaactcc acctaaattt cctacaaatg aaaatttagc tgttgcagaa
    18121 gcaaattctg acttattaaa ccatctccct tgaattttta aaatacaaag taatattact
    18181 gatgttcctg cccctacaat tacacgagaa atatatgcta attctatact ttgaatcaaa
    18241 ccaaataaaa ttgaccctaa acttgccatt attattccaa gactgctaat ttttcttgct
    18301 ccatatttat caatcaaaat tcctgcaggt atttgcataa atgcatatgc atataggcaa
    18361 aaggatgcta tatttgaaat ttgtatagaa ttaaaaccta actcatttgc taaattgtct
    18421 gaaacaacag cagttgacat ccttaaaaat aatactaaaa caaatgaaaa tgtaattata
    18481 ccccaaacac tccacttagc ctttgtataa cttttcatat cttctccctc aaatccatat
    18541 atttttaaat tttcttgata taacttatat aaagtgtata gttattctta caaattttta
    18601 cacgcaatat cattatacaa catatgtata tatttaacta gaaataaaac actattcata
    18661 ttatatacat gaattaacta cataaataat aagagctatc ttttaacatt actcaaaaga
    18721 tagctccata taaaaacatt tattctaaat ttaatttttt atctaatata aattttgtag
    18781 cgaaaaataa cgctattgca actacaagac taccaagtat tgcaagcata aaactaggac
    18841 tctgataaag atgatatacc atatttgtat tttcatttgg taagatgctt ccaactgcat
    18901 ctccaacaat tgatattact atatttatag ctataaaaga agcaaatgcc actatgtttc
    18961 tatgtttatt gaattttggt aattgtccca ttgagattga tgtatataat aacaaaataa
    19021 atgatgaata tgaaataaac attactacta ctaataataa aatatctaaa aagttttcag
    19081 aaatccattt aaaactagtg ttaaacaaat ctaaaatttc aggtagtaaa attccagaag
    19141 taccaaaaag cataagaact ccaaaagtga atactgcaac tataaaacta agtacagcgt
    19201 atataagtgc tgttatacat ttagataata taagtgaact agtgctaaca ggaagagtaa
    19261 acattaaata accttcatcc cccaaaagat tttttctaaa cctttgaatt gtaactacaa
    19321 tagttaaaac tcctaatgcc ataaaaagac ttgttaatac tagtattcct attccctgta
    19381 cctgaagtat atttgtattc ataaatattc cattaaatac agctactatc aatatcgcta
    19441 tatataacgg tataaatatt cttccacttg cttttaactc atattttaat aatttaccta
    19501 acatttaaat tcctccctaa ataatgcatc tatagatttt cctttttctt ctcttattgc
    19561 ttcaacgtct ccttgtaaca ctatttctcc ttttgatatg aatattatct catcacatat
    19621 gttttctatt tcacttatta agtgggtagc aatcagtaaa gtactatctt cagaataatt
    19681 ttttaatata gtcttaagta tatatgacct tgcagctggg tcaacaccac ctataggttc
    19741 atccaatata tatatgctcg catttcttga cattactaaa ataagttgaa ctttttcttt
    19801 agttccttta gacattgtct ttagcttttc atttacatca atatctaagc ttttaatcat
    19861 ctcatttgct cttctaacat caaaatcact ataaaaatca tagaaaaact taagtaaatc
    19921 tgataccttc atccaatcat ttaaataagt tctttctggt aaatatgaaa ctattttttt
    19981 agtatctatg gatggtttca ttccatttat cataatttca ccattatcag gctgtaataa
    20041 tccattcatt agcttaatca ttgtactttt gccacttcca ttaggtccta acagcccgac
    20101 tatttttccc tttggtatat ttaaatcaat attttttaaa acattcttag ttccataact
    20161 cttgtttaca cctttaaatt caactattcc atcttgagaa acattgctta tatttttatc
    20221 cattttataa ttctcctttc agattttctg taataagttt tagcatttca tctctatcat
    20281 atccaagctt ttctaaggtt atttttaatg tatttatctc cacattagct atttcctctt
    20341 tcatagcttt tattttttcc ttatcatctg ttacaaatct tcctttagtt ctctgactat
    20401 aaactaaacc ttgtctttca agttctgtta gagccttttg cattgtattt ggattaactt
    20461 ccgcatcttc tgctagtgct ctcactgttt ctaatttaga tcctatttta atttcccctg
    20521 aaattatttt taactttaaa tgttctacta attgtatata tataggtttg ttattatcaa
    20581 gttcccattc catgttcatg cctccttatt atattattgt attaagttgt taatacaata
    20641 atataataat acaaagtata tgtcaattac tttttagtaa aagtataaaa aattttacaa
    20701 aaaaaatgaa cactacatta gagtccattt ttaacacaat ttattaaatt ctttatatta
    20761 cagattaatt accctcttat caagttggat acaattaaat gttcaaattc ttcaattggc
    20821 attggctttc caaacaaata tccttgtgcc atatcacact ttacttcttt taaaagttta
    20881 aactgttctt ctgtctcaac tccttctgat atgactttca tacccaactc ttttgccatt
    20941 tctattactt tagtaacaat tattttgctt cttttactat tataagtttc cataataaat
    21001 ttttggtcaa gttttaagat atccatagga atttctttta acatatttaa tgaggagtac
    21061 cctgaaccaa aatcatccat agatattaaa aatcctattt tttttaaatt attcattata
    21121 tcaattaaaa tatcaaaatt atcaaatatg atactctccg ttaattcaag ttctatatac
    21181 tttgcaggta tattgtattt atcaattata tttttgtatg tttctataaa attattgcaa
    21241 tataaatgta tccttgaaac attaactgaa acagtaactg gatttattcc tgcatcaatc
    21301 cattttctaa ggtatataca tacttcttca agcacatata aatctaaatt tataacaaaa
    21361 ccgtttcttt caaaaaatgg gataaattca tttggaggta ttagcccttt atcaggacgt
    21421 ttccacctaa caagtgcttc tgaaccttgt atctctcctg tattaagttc tatcttaggt
    21481 tgtaagtata taataaattc tttattttct agacttgaaa acattgtatt ttccatctct
    21541 ttttctttta tctcttggtc atgaatctta ttatcataaa atgcatatga acttttatgt
    21601 cctccctttg ccattttatg aggtatatta gccctatcta tgattgagtt gatatctcta
    21661 tcttctggaa gtataaaata gattccacaa cttaaaacta acttatattt ttcttcttga
    21721 ttattattaa acaagtctaa ttcttcaaat atagttttta acctctttgt tatatcttct
    21781 tcttctatat atttcataat tataataaaa ttatccgcag atattcttgc aaacatttcc
    21841 tcttcaaata tattactctt aataatcttt gttaaattac gcaaaatttc attcccaact
    21901 tcatatccaa ataaatcatt tatatattta aatttatcaa tatctatata taataaagca
    21961 tactttactt gagtattttt acatagtatt ttgttagcat caattacaaa tttatctata
    22021 gtatttgctc ctgtaagatt atctgtatat gctatccttt gcaatttaat tttaaaattt
    22081 aatttatcat atattatata gaatataagt attacacata ttatagccat aataagtatt
    22141 atttgtattg catgtttgct aataagctga ttattagaca caatagactc aaagttattt
    22201 atggagttgt ttttatagag tttgcataaa gaatagactg gtgttgtgta tgtaatttgt
    22261 aaaattatac aaataaataa aaaaaacatc aatttttttt gtaaattcct cataaaaatt
    22321 tcctcccatt aaaacacctc tattttatta gcaaataagt acatctcaaa agtttccatt
    22381 tcttgtaaaa atggatagtt tatatctact ttaattttgt aaaactttct gctactataa
    22441 ttatagaatg tagcatattc attgtcttaa ctattgattt ata
//
LOCUS       DAEMIK010000061        22217 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEMIK010000061 DAEMIK010000000
VERSION     DAEMIK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.061"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(39..1352)
                     /locus_tag="KPK87_001930"
     CDS             complement(39..1352)
                     /locus_tag="KPK87_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2538081.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            complement(1585..2922)
                     /locus_tag="KPK87_001931"
     CDS             complement(1585..2922)
                     /locus_tag="KPK87_001931"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG2538082.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            complement(3071..4876)
                     /gene="lepA"
                     /locus_tag="KPK87_001932"
     CDS             complement(3071..4876)
                     /gene="lepA"
                     /locus_tag="KPK87_001932"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG2538083.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(5158..5409)
                     /locus_tag="KPK87_001933"
     CDS             complement(5158..5409)
                     /locus_tag="KPK87_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538084.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(5440..6459)
                     /locus_tag="KPK87_001934"
     CDS             complement(5440..6459)
                     /locus_tag="KPK87_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG2538085.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSRENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(6477..7430)
                     /locus_tag="KPK87_001935"
     CDS             complement(6477..7430)
                     /locus_tag="KPK87_001935"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG2538086.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(7773..8549)
                     /locus_tag="KPK87_001936"
     CDS             complement(7773..8549)
                     /locus_tag="KPK87_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2538087.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(8583..9104)
                     /locus_tag="KPK87_001937"
     CDS             complement(8583..9104)
                     /locus_tag="KPK87_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538088.1"
                     /translation="MDIAKLILNPARLRILQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            complement(9514..11166)
                     /locus_tag="KPK87_001938"
     CDS             complement(9514..11166)
                     /locus_tag="KPK87_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2538089.1"
                     /translation="MKIYKKLLTTGISFLLVISAFPIVSNALSSIDSIKGKDKYETSA
                     IIADKQNYTKAILINVDNSLADGLSASGLAGVENAPILLTKKDEIPDSTLKRLNNVKK
                     VYIIGGNNSIGSKVDNLLKKKNIEVERIEGKDRLSTSYKVSDKILELKGTSGNVLVAN
                     GFKGEADAISAASVAFKNSIPVLLTNGSDMPELKIKGDKIFAFGSTNTMSNQLVEKLG
                     AIRLGGIDRYETNKKIVQQFYGDAKEFYVASGSDLVYPLIGSTLAERKPIILVGNGSN
                     KSILKGATKITSIGNVDASIITQCLNVTNNIGDINTGMVKTNTNKEYPIKGMLAKFGL
                     NTTGKIGWALMYGGNGNGVELRADGKYYHINRGNTALGAYAAALAGEKYHSLDFGDLD
                     PIEIIADKEEISYEKAKKEIVVIRNFLNSFDWQNASDLEKATRAGKLVTEADYVMGNY
                     NIYTNLLEKKSVCEGFAKSFYVLTRLMGMDSIYQEDGRLNHAWNYVKIDGKWYEFDGT
                     EAGSYKDLGIKVEFNSSKLEEATKQMPKYYDAKALSVLGFNQ"
     gene            11600..11866
                     /gene="rpsT"
                     /locus_tag="KPK87_001939"
     CDS             11600..11866
                     /gene="rpsT"
                     /locus_tag="KPK87_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG2538090.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(12053..13096)
                     /gene="holA"
                     /locus_tag="KPK87_001940"
     CDS             complement(12053..13096)
                     /gene="holA"
                     /locus_tag="KPK87_001940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG2538091.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(13050..14303)
                     /locus_tag="KPK87_001941"
     CDS             complement(13050..14303)
                     /locus_tag="KPK87_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG2538092.1"
                     /translation="MFVVAISFIITNNTKNDEELNDKIITIQGVVKGKIEKKRYNQYK
                     VGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGRYIKSTGYKGIVYINNY
                     KIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEVENLTEEQKEIFTRTGT
                     SHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYCIMVGASPSIIRSIAFV
                     MIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLSFLATLSILIFYNKINS
                     IIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPFVGVIMYLSIASLIVFK
                     VSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIENPKFYIVVIYYIGVFFYIFY
                     IEGKEIKEQENELQGYYKECKREKL"
     gene            complement(14466..15650)
                     /locus_tag="KPK87_001942"
     CDS             complement(14466..15650)
                     /locus_tag="KPK87_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2538093.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKIYIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            complement(15659..16564)
                     /locus_tag="KPK87_001943"
     CDS             complement(15659..16564)
                     /locus_tag="KPK87_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG2538094.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            16752..17588
                     /locus_tag="KPK87_001944"
     CDS             16752..17588
                     /locus_tag="KPK87_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2538095.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKKLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            complement(17733..18437)
                     /locus_tag="KPK87_001945"
     CDS             complement(17733..18437)
                     /locus_tag="KPK87_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG2538096.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLG"
     gene            complement(18588..18684)
                     /locus_tag="KPK87_001946"
     tRNA            complement(18588..18684)
                     /locus_tag="KPK87_001946"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18648..18650),aa:Sec,seq:tca)
     gene            complement(18928..19767)
                     /locus_tag="KPK87_001947"
     CDS             complement(18928..19767)
                     /locus_tag="KPK87_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG2538097.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            complement(20016..20690)
                     /locus_tag="KPK87_001948"
     CDS             complement(20016..20690)
                     /locus_tag="KPK87_001948"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG2538098.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            complement(20727..21098)
                     /locus_tag="KPK87_001949"
     CDS             complement(20727..21098)
                     /locus_tag="KPK87_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG2538099.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            21165..>22217
                     /locus_tag="KPK87_001950"
     CDS             21165..>22217
                     /locus_tag="KPK87_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG2538100.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNK"
BASE COUNT         7377 a         3726 c         2395 g         8719 t
ORIGIN      
        1 tcacctttac ccaaatttat ctaacttaaa ttaagtagtt atcttttttt catataataa
       61 taaactggta aacctaatag tgttataaat attccgccta aagatatgac tgttgaagtc
      121 aatatttgat ttattattac aaacagtcca cctaatatag ctataattgg tattattgga
      181 tataaaggga ctttatatgg tctgtgtaaa tctggctttt gtcttctaag cctcataaca
      241 cctataaaag tcaatacata aaatacccat attgaaaata ttgttaagtc tgtcaaaaga
      301 ttaaattgac cagataatgc atatacacag gctaacacag ttataagtag tattgaattt
      361 acaggagaac ctccagaatt tacttttaaa agagttttag atgctggtaa agttcccatt
      421 tcagctaatg tataaggtat ccttgaacct gttaataaat atccattcaa tgtcccaaat
      481 actgatatta atatacccac agttattact tttccaccta tatttccaaa caatcttgtt
      541 gctactaatg tagctgggga agtaactgtt gctaattcac ttgctggtac tacccaaaga
      601 taagctatat ttattataat ataaactgcc ataactaatg ataaaccacc aactattgct
      661 cttggtaaat ctttctctgg agacttcatt tctcctgata tagctccaac atttatccaa
      721 ccatcatatg caaacaaagt agctattaat acttgtccaa gtgcatttga tgcatttaca
      781 ccatctccta caaatggatt taatacttca ctactttgac ctttaataaa tccaaagact
      841 attattaaaa ttaaaggtat catctttcca atagtggcta ctgtctgaac ttttcctcct
      901 aaggatgaac ctattatatt taataacgct aaaaatagta taactccaat tgctatagga
      961 agtacatttg ccatattatt tgggctcatt cctaaaagtt cagatgcttg ttgagcaaat
     1021 attactccta atgccgctga tgtacctggg aaaaataata cagtttgcat ccatccagtt
     1081 aaaaaaccta acttttctcc atatatttct tcgatataaa tcatcatacc accagttttt
     1141 ggtatagcag ctgaaatttc tgtagctgta agcccagcag ttattgttat aaaaccacct
     1201 aaaagccacg ctataatacc tagacctgga gctccattag ttgtagtgta tattgcttgt
     1261 ggcttaaaaa aaactcccga acctataacc atacctacaa cagtagaaag agctgctgaa
     1321 agaccaatag tcttttgtag ttgagtatgc atactgtccc tcctcactgt tttacagtat
     1381 ataaaatatt aattttataa atttatggtt ttagtattcc caaagttttt ttttatatta
     1441 gtctatagtc ctagagtaaa tgcctatgca tttaaaaatc atacaatcgt aacttctaaa
     1501 atattctcta aaaattattt attttatcta aataagtatt ttattctatg ttttttaaag
     1561 taattaaatt ttttatattt gaatctaaaa tgtatatata tttaatgctt tttcttttag
     1621 tatttctttt tcatttaact ctggattatt tatgactatt tcaagcatct tatttaaaat
     1681 atatcctata tctttacctg gttttatacc tatagatatc aaatcttttc cattaatagc
     1741 taaatcttta agattaaagc actccttttg atttattata tgtttaaatt ctttttcaca
     1801 ctcaataaga tttaatatct tttgttttgc atactttgga ttttttgcaa gtgtatcagc
     1861 ccaattaact tttattaaat cttcaaataa ttcaacatct tcaagtttat ttaataactt
     1921 tttaattatc ttccttttag gttctattct ataatcatga tattgtatta atattaaaac
     1981 tttattaata gtataattat catacttaag tcttttcaaa atcttctttg caatctttac
     2041 agattctcta ctatgagtat aaaaatggtc aacaccattt ttatcagttg ttttagttac
     2101 ttttttacct aaatcatgaa acaacattgt aagttttaaa tacagttcat cttcaattac
     2161 ttctgtagct attaaagtat gtttttgtac atcatagatg tgatatggat tgtgttgagt
     2221 aacatcttct aattcacata tttcaggaat tataaatttc aacaaacctg ttgattttaa
     2281 taagtttaat ttagatgaat cagatactaa tattttatta aactctgtct ggattctttc
     2341 aatagaaaca ttttttatca aatctgcttt tttatgaata ctttgcttag taccttcagc
     2401 tattttaaaa ttgagttgtg ctgaaaatct aactgctcta agcattctaa gtgcatcttc
     2461 ttcaaatcta tctactggat ttccaacaca acgtatatac ttattttgta tatcttcata
     2521 gccattaaat gggtcgacta atcctgtctt tgaattgtat gcaattgaat tgatagtaaa
     2581 atctcttctt gacaagtcct taactatgtt atttgtaaat tctattgaat ctggatgtct
     2641 accatcacta taatttccat caattctata agtcgtcact tcaaatggtt catgttcaat
     2701 cataactgtt acagtcccat gttgtatacc agttggaatt gttttttcaa ataattctac
     2761 taccacttct ggtctagcac tagttgttat atcccaatcg tttggaactc tttctaataa
     2821 acaatctcta acacatcctc caactatata agcttcataa ccattctttt ctaattcttt
     2881 aattatatag tctacttttt taggaatttc tatattaatc ataaatatca cctttttatt
     2941 cttctttaat attcttactt atattctcta tgagtttact taaaatatac cataaaaaag
     3001 ctagactaat ttagaaataa ttctaaaatc actctagctt ttaaatatct ttatctaaat
     3061 ttataaggat ttactcatct aatttaagca cagacataaa tgctttttga ggaacttcaa
     3121 cagaaccaat ttgtctcata cgcttcttac cttctttttg tttttcaaga agtttcttct
     3181 tacgagatat atctccacca taacatttag caagaacatc ttttcttaga gcgcttattg
     3241 tttctcttgc aataacctta ttaccaacag cagcttgtat tggtatagca aactgatgtc
     3301 ttggaatttc tccttttaat ttctcacaca ttgcctttcc tctagggaaa gcacgtgtct
     3361 catggacaat aaaactaagt gcatccactt gctctttatt gattaatata tcaagtttta
     3421 caagtgtaga tgctacataa cctttaacct cataatcaag agaaccataa cctctagttc
     3481 ttgattttaa agcatcaaag aaatcgtaaa caacttcatt gagaggaagg tcatagtgaa
     3541 gcattactct tctatcatct atatattcca tatttaacat atttccacgt ctttcctggc
     3601 aaagctccat aacaacaccc acatagtctt taggaactat tatatctcct tttactatag
     3661 gctcttctat catctttatc tcagatggtt ctggtaagtt tgcaggattt tgtatcatta
     3721 caacctctcc atccattttt gtaactctat atataacaga tggagctgta gtaattatat
     3781 caaggttaaa ttctctctca agtctttctt gcattatctc catatgtaaa agacctaaga
     3841 aaccacatct aaaaccaaat cctagtgctg ctgaagtctc agcttcatat tcaagtgcag
     3901 catcatttac ttgaagcttt tcaagagcat ctctaacatt ctcatacttt tctccttctc
     3961 ctggatatat accacaatat accattggag tagctttttt gtatcctggt aaaggttctt
     4021 ctgtaggatt atttgaatct gttattgtat caccaacacg acaacttctt atatctttta
     4081 tactagcagc aatataacca acatcacctg ctgataattc actaagttca gtttgacctg
     4141 gagccatgac accaacttca gttacctcaa actttttatt ggtattcatc atttttattg
     4201 tcataccctt tttaacagtt ccttcaaata ctctaacata agctacaaca cctttgtaag
     4261 catcatagta agaatcgaat attaatgcct ttaatggagc ttcattatct cctttaggtg
     4321 gaggaacatt ttttacaata tcttctaaaa catcttctat gtttaaacca gttttagctg
     4381 aaataagagg agcttcactt gaatcaagcc ctattaaatc ttctatttca ttttttactt
     4441 cctctggtct tgcacttggt aaatctattt tatttattat tggtaatatt tctaaatctt
     4501 ggtctatagc aagataaaca ttggctaaag tttgtgcctc aacaccttga gctgcatcaa
     4561 ctactaatag tgctccttca catgcagcta aacttcttga tacctcatag ttaaagtcta
     4621 catgtccagg tgtatctata agatttaaat aatattcatt tccatccttc gctttataca
     4681 ttaatcttat gttttgaagc tttatagtga ttcctctttc tctttcaagg tccatattgt
     4741 caagtaattg acttttcatg tctctttcac taacaagccc agtttgttgt attaatctat
     4801 cagctaaggt agactttcca tggtctatat gtgcaattat actaaaattt ctagttctac
     4861 tttgtttatt gtccacctta aaattccctc ctcttgatta ttcttatagt tattatttta
     4921 cattatctaa aaactcttta tataccaaat ttaaaatttt tgaatataaa ctaattggca
     4981 ctaaacaaaa ttatccacat tatatgtgga ttatcaagtc taacactaaa tattatataa
     5041 tatcgtaaac ttaaagtaaa tcctataaaa tacagttaat gaatttttat tttatacatt
     5101 aaaaatatat aactaaaata aggcttattt taatcaataa accttattca cttattacta
     5161 cttattaaat ttttttatga tttcatgtat atcaaaattt ggtatgtcaa aatcaaattt
     5221 tgttatatca aatttaatac ttggaaaatc atattgtttc attcctagca tactttttgc
     5281 attactgtcc actataaata cacaaactga tgtacatata atcatagtaa tcaaaaataa
     5341 gattctcata atatacttgt attttcttag tttactttta ctttctatat ttttttctaa
     5401 tcggctcatt tagcatgctc ctaattataa cttaaagctc taattttttt gtttaaaata
     5461 ttcatccaaa atctcggcta aatactttgt agtagcctta gattcttcta cacttgttgc
     5521 gttattccct atttctacta acatatgatt cttaactgca aactgattga atctagcata
     5581 gtctttttct actattggag atactagacc aggatacttt ttctcagcaa aagcagttat
     5641 atcttctgct aaagctctaa gttgttttct atttgtattt ttacctccta caacaaagaa
     5701 gaactttgat actctctctc catttatcat agttgtatat tttaaatggt ctttttcctt
     5761 agtggctgga ttagttaaat ccctagcatc tctatgtaag tctatcgcta tatccactga
     5821 gttatattta ggtaatatac tttgtattgt ttttaagcta cttgcataag agttattata
     5881 agatggatag tcatggtatt tagtagtatg gactacaccc catcctttgc tatctaaagc
     5941 actagttaat gcacttccta cttccattac tgaatttttc ttatctcttg aatgataatt
     6001 tccatcaggt gaatttgaat aagtctcaca tccatgagta tgatatatca aaattcttgg
     6061 attttctttg gttactctta aatcatcctt ataatctgaa ctagatgttg tgttagtttc
     6121 agttgtgtct gaattttttg actcaatatc tggaacattt tctttaccaa cataaagttt
     6181 tatatattct ttttcactct cttttttatt atcaacttta cttgcatctt tagatttagt
     6241 gtttccttct ttactttctt ccttattttc cctacttgtt tctttatttt tattattctt
     6301 tttattttct gtatcatttc cttcgacttt agcttctgga taagatgaat ttactaaaaa
     6361 ctttaaaaaa tcatcttgat tcaaagctaa tgattttcct ggtaaaatgc atactaatat
     6421 aaaagtcaag gttactactt taatgcattt tttaaacata attgctcctc cttattttat
     6481 accaatctac ctggatgtaa agacctattt aagccttcac ttattattat agatagattt
     6541 tcgattgtat catctatatc ctttggtgtt actattaagt tcttgtcata tgggtctaac
     6601 gaatctttta ttagatgata tttttcttct tctttaagtt ttttcaacat ttcataaaaa
     6661 ctttctgttt cttctgattg agctattaaa ttgtctattg ccatatctaa gacatctatt
     6721 gtaagagttg ctgcatcaac cactgttgga acgcctattg caataacatc cacgcctaaa
     6781 gtttctttag ttagagattt tctagtattg ccaacacctc caccaggtga tattcctgct
     6841 gttgatatct gtatagtaga gtttactctc tccatttttc ttgaagccaa tgcatctatt
     6901 gctactactc tatctggctt aaccttctca accaatgatt ttactatttc actagtttct
     6961 attccagtaa cacccataac tcctgggctt aaagctgata cctcagtgaa atcatcatca
     7021 tagtctttat tataattttt aaaaagatgt ctagttacaa gtgtttttga tacactcttt
     7081 ggtcctaacg catcagaagt aatatttcta tttccaagac caattaccag ggttttttta
     7141 tttttgtctt ggccaaatat atctactaac tcctctttta aataacttat catttcttct
     7201 cttgactcat catcatcaaa cttcataaga ttactttcca gagttatata ctttcctata
     7261 tctttattca ttatctctgc gccttgcctg tctattactt caactttagt tacaatgcaa
     7321 ttttctaatt ctttagtttc tatttttaca ccaggaatac tacttccttc ttgagacttt
     7381 tcacacattt cactagcttc taaagctaaa tctgttctta cactaatcat ataaacctcc
     7441 agtataaatt aaatgctaat tataattgtt aacaaaattt agtattttat aacaagaaat
     7501 tgtaaattta tttaaaacaa gtaaaattaa ggctttcaat gtaagaaaat tgggtttagt
     7561 cagtgatttg tcagtaaacc ttcttatcta tcatttattt acaaacagaa aaaggtagca
     7621 actatttata taggcgctac ctttaatcta tttataattt tcctattaag ctttcactaa
     7681 tatattaact aaaataactt ttttatattt atacctaatt tatttactat attaaaaaac
     7741 agcaataatt atacaataat cacctaacac ctttattcct tttggaaaaa tggttctgca
     7801 atttccaacc attcatttgg aagataaagt gataaatatc catgattata gctcttagct
     7861 tcataaatct cacaagaagg cacataagac tgaaacagtt ttgcagattt tttgacacac
     7921 ttcatttctt tttctccata ccagtacatt acttgtgctg tggttttttt aacattctct
     7981 ttaagtgtat agttcatcat ataagtacga tacatattat agaatgtttc ttttcttaca
     8041 tgtggcatat ctgtcatata atacgctttt atttcatctg gatattgcat attttcaggt
     8101 aacaattttg gcatcatagc aatttgaaag cgacaagcct tctcactaaa caaaagtcct
     8161 ccaaaaaatc gaataccagc aatacaaaac cttgccataa gaggtcttgg gtagcaaata
     8221 ctaccatcaa taattgcttt tttagtaata ttaggttttc gagataaaag ttccatgaca
     8281 atttgaccac caagtgatac accacacaaa gcgaaaagat gtccactgca attttcatct
     8341 atatatgata tcaacttatc tgctgtatcc tctgtagaaa tgtatgttgt ttcatattct
     8401 tctccatgac catccaatgt aggaagtatc acatgatatt tgttagacaa aacttctgct
     8461 tgtcgcaaat agttccacca tgcattccct cccccatgaa tcaacataat atgtggtctg
     8521 tttttatttc caaactcatg aaatttcata tataacctcc ttttataaaa atgggttgca
     8581 tgttattttg ttttatcatt atcagctaca ggtggagaag atataattga aagttttcta
     8641 agttttagat ttgatttagg tttttgttct atatatggtt tcaataaatc agctatactt
     8701 ttaagaagat tttcatattc attatcactc atatatagta atgctgaaga gaaaaataca
     8761 ttatcctttt tatggtcttg ctcttttctg ctaaagtatt cattcatttc ctgcatcaga
     8821 cctacataaa atgctgtaga aagtgcaacg aaggtctctc cttccataga tgttgtatat
     8881 tcctttagtg cataaacctt ttcaatagta ccacgcacac gattttcttt aattacttca
     8941 atcatattgt tctcttctaa aatttttaca tggtgataaa ctgttgcacg aggaatatca
     9001 tttaaatact ttacaatatc agatgtgcga acactgctat gaagtcgaat atattgtaaa
     9061 atacgaagcc ttgcaggatt taaaattagt tttgctatgt ccatcttatc agccctttct
     9121 ttagtctata aaaatattat agtctatttt tttagactat tcaagtctat gatataaata
     9181 gtaattacaa ctataataaa tgctattcta tataaacact gacattaatt gcatataacc
     9241 ttccaacata ttagttatat tgtttggtat atttatcata tacaccaatg taatttaatt
     9301 tcagtttatt taattccaga acgcttaaag tttcttttgt aataacattt gtattactat
     9361 tttcattttc tatttatata aaaatatata aataaatttt ctatgccatt taagtacatt
     9421 atataaaact atcaaatagt caacagtaat tattcaacct taaaaataat tttggaacat
     9481 ttaaaaagaa agactctttg aagagtcttt cttttattga ttaaatccta atacagataa
     9541 cgcctttgca tcatagtact taggcatttg ctttgttgcc tcttcaagtt tagaagaatt
     9601 aaattctact ttaattccca aatctttata tgatccagct tcagtcccat caaattcata
     9661 ccatttacca tcaattttta catagttcca agcatggttt aatctaccgt cctcttgata
     9721 tattgaatcc attcccatca gtcttgtcaa tacataaaag ctttttgcaa aaccctcaca
     9781 tacacttttc ttttcaagga gattagtata tatattataa tttcccataa catagtctgc
     9841 ctctgtgacc aattttccag ctctagtagc tttttccaaa tcagaagcat tttgccaatc
     9901 aaagctattt aaaaaattcc taattactac aatttctttt ttggcttttt cataagaaat
     9961 ttcttcctta tctgctatta tttctatagg atcaaggtct ccaaagtcca gactatgata
    10021 cttttcacct gctaatgcag cagcatatgc acctaacgct gtattacctc tatttatatg
    10081 ataatatttt ccatcagctc gaagttcaac tccatttcca tttcctccat acattaaggc
    10141 ccacccaatt ttaccagttg tgttaagacc aaactttgct aacatccctt tgataggata
    10201 ctctttattt gtatttgttt tgaccatacc tgtattaatg tcccctatat tattagttac
    10261 atttagacat tgtgttatta tagaagcatc aacatttcct attgatgtta ttttagtagc
    10321 acctttaagt atacttttgt tacttccatt tccaactaat ataattggtt ttctttcagc
    10381 tagagttgag ccaattaatg gataaactaa gtcactacca ctagctacat aaaattcttt
    10441 ggcatcgcca taaaactgtt gaactatctt tttattagtc tcatacctat caattccacc
    10501 taatcgaatt gctcctaatt tttctacaag ttgattactc atagtatttg ttgaaccaaa
    10561 tgcaaatatt ttatctcctt taattttaag ttcaggcata tctgaaccat tagtaagtaa
    10621 tacaggtatc gaatttttaa atgcaactga ggctgcgctt atagcatcag cttcaccttt
    10681 aaacccattt gctactagta cattacctga agtacctttt aactctaaaa tcttgtcact
    10741 tactttatag cttgtactaa gtctatcttt tccttcaatt ctttcaactt caatattttt
    10801 cttctttaat aagttatcta ctttgcttcc tatagaatta tttcctccta ttatgtacac
    10861 ttttttaaca ttattaagtc tttttaatgt tgaatctggt atctcatctt tcttagttaa
    10921 taatattggt gcattttcaa ctccagccaa tccacttgct gataatccat ctgctaaact
    10981 attatccaca ttgataagta tagctttagt ataattctgt ttatctgcaa taatagctga
    11041 agtttcatac ttatctttac cctttatact atcaattgaa ctaagtgcat ttgatactat
    11101 tggaaaagca gaaatcacta gcaagaatga aattcccgta gttaataatt ttttatatat
    11161 tttcataata taaatacctc ccagatatta tagttccact taaataatat actataagat
    11221 gaaaaaaatc accttgatta aacatataaa tttatatttt tataaatttt ttattaaatt
    11281 caaaaaaatg agtaatttct tgtattatct tataattatt ggaaatacta tcaaaatgta
    11341 tgttaatagt ttgtattatc tcaaatattt gttacaaaat gaaatattaa ttatatatgt
    11401 caaaatattt aaaacacaaa atataccaat ttaaaaacaa gatattgcaa attatttata
    11461 cacaatttac agtctaaaaa atcaaaaaaa aacattatta aaatatataa ataataatcc
    11521 ttgattttta gatttatttc tgttaaaatc taatatgttg acgatgaata tgataaaaaa
    11581 atacagggag gtgaacacgg tggcaaatat aaaatcagct aagaaaagaa taagtgttat
    11641 cgaaaagaaa actgctttaa atagagttag aaaatctcaa ataaaaactg caataagaag
    11701 attcgaagat gctgttgcag ctggaaacag agaggatgct gttgctaagt tccaatacgc
    11761 tcaaaaaaga atataccaag tagcttctaa aggaactata cataagaatg ctgcagctag
    11821 aaaagtagct aaattagctc aaaaattaaa cggtatgaac gcttaattac cagtttatat
    11881 ttcttatata taataacccc tatgtagggg ttttttattt tgtaaattta tacaataaaa
    11941 aataaatagt tagacatatt atagttgatt gtatatcaaa cttaatttag cttacatcta
    12001 ctctgaacta taacatatct tgctatttat tttttattaa tatatagatt ttttaaattg
    12061 cttctctttt acagtatcta ctaaccaaca tttcaactgc taaagtatct cttataagac
    12121 ctgtctttat tttgtaatca ctttctaata tatagtttaa tatttccaca attatatcat
    12181 cagaaaaatt tttagtttgc tttaatgctt ttcctacaac aaattgatgc atttttaatt
    12241 tatcagctat aagttttgtt gaatatccat caagttgtaa ctgtcttact tgcataatta
    12301 ttttaaattg tcttgctatc atagaaaata tacctaatac agattcgcct tcttgtatca
    12361 tatcatttaa tattttcatt gcattagaag cattttgttc tccaatataa tcgattaatt
    12421 tgaatatatc attctcaact tttttttgag ataatttatc aattacatca ttggttacat
    12481 tattcccttt accaacaaat gaacttattt tatttatttc attttctaaa tcagataatg
    12541 tcttttcact actcttatct ctgtatcctt cttgttcaat aaaatacatg atttgagaat
    12601 tatctattat aacatcattt aaagcaaaag atttttttat ccatttaaac aaatccatat
    12661 cagaaagctt atcacaatca aaaacaattc cattgttccc tattttctta actaatgatt
    12721 ttcttttgtc tacatctcca tatacaacaa aaactatagt tgtggtgtct ggaatattgt
    12781 ctaaatgctc tattaagtat ttctcatcac tatctgtaaa attctttttc tttcctttta
    12841 atagttcaaa gtcttttatt attactatct ttctatcatc cataaatgga agtgtttcta
    12901 tagagcttat aagctgattt aagactattt ccttcccatc tattatatct aaattaaaat
    12961 caagcattcc ctcgtttaaa ctatctttaa atgcttttat tgcattctca attaaataat
    13021 actctcttcc ataaaaaaga tacatttttt caaagttttt ctcttttaca ttccttataa
    13081 tatccttgta attcattctc ttgctccttt atctcttttc cttctatgta aaatatgtaa
    13141 aaaaacactc ctatgtaata aataacaaca atataaaatt taggattttc tatattaatg
    13201 tatgcaaaac ttaaattgct gattttttct agtaaaaaaa atatgctttc tatcaatgta
    13261 ctattaaaaa aagatataat tttagctatt actacactta ctttaaatac gattaaactt
    13321 gcaatactta aatacattat tactcccaca aatggtacta taattaaatt tcctataatt
    13381 gaaagcaatg gtataccttt aaatgtataa taaattatag gtaaagttaa tatatttgat
    13441 gatattgtca atgataccat acttaatttt ataatactat ttattttatt atagaatatt
    13501 aatatagaaa gtgtagctaa aaaagacaat tgaaaactta cattatatat aacatagggg
    13561 ttattcataa ttaaaattat cccaattaga gacaatgccg atattccatc tttctttcta
    13621 tcaataaata ttgataaata aaaaatcata acaaatgcta tagacctaat tatggatgga
    13681 gacgcaccta ccattataca atataaagtt ataaaaatcg acagtattaa taatttatac
    13741 aatttattta tacctcttaa tagaaaggat ataattacac ataatattcc tgtatgaaga
    13801 cctgatatag atattacatg actagttcca gttctagtaa aaatttcttt ttgttcctca
    13861 gtcaaatttt caacttctgc taatagtata gaatttataa aatctgagtt tttcttatat
    13921 aagtacctat aagttttact aatataaaac tttatttccc ctattaaaga gtaaagttta
    13981 tttttatcaa taattttata gttatttata tacactatac ctttatatcc agtacttttt
    14041 atataccttc cataatcaaa atcatcatac ttcattttat ctaaactttt gaatttacct
    14101 gtaatattaa ctttctgacc aatcttcaaa tttttatttt ttgtataatc atttattaga
    14161 aaaaccccta ccttgtactg attgtatctt ttcttttcaa tttttccttt gacaactcct
    14221 tgtatagtta ttatcttatc attaagttct tcgtcgtttt ttgtgttatt tgtaatgata
    14281 aatgatatag ctaccacaaa cattaatata attaataatg gtcttcttat ttgaattata
    14341 atcactccta tataaaagta tattatttag attattttca aatagaaaat ttttaaacaa
    14401 gataaattgt tatctaaaca aaaaaatacg tcaaaagatt tttaaaatct tttaacgtac
    14461 tctacctata tatattcatt tatatagtta ttttttttag aaaaaataca attaagtgtt
    14521 tctagtgttt cttctgaaac atcttttata tcatttaaga aaatagcttc atttacatct
    14581 atataagaaa atgataactg tgttaaggta tttatatcta atttaatatc aaaatcttct
    14641 tgaggctttt ctgcaccttt tttatctaac tgctctacag ataccttctt attctgtata
    14701 taaattttaa atagaccatt attttcatct ataaacttgt cttctattaa taaattaaat
    14761 gagctattaa tatccttctc aatatctata tcttcaataa acttctttac atttataact
    14821 cttcccatca taaaaggttt tatttttata tcactatctt tagggttgtc taatataaat
    14881 cttatcttat ctattgtagg agtagatata gtaactatct tacattgagt attgtgatta
    14941 tatataaatt taagcattga tttaagggca tctatatttt tataaaataa ctctctgaca
    15001 aacattttat cttcctgaag aaaataaact atatatccat ctttttcatt tccttcatga
    15061 atgtataaaa atccatcttc actttgaatc tctttaaata agttttcata ataatgttca
    15121 tcttttaaca cattaccatt aacctcattt aaaaaagttc tatctatttg tatcaaatca
    15181 tcaatttgtg ataaattaga ctttatcatt tttccacttg acttaaaatt ttttaaatca
    15241 tctgtgttaa tagtatattc aatctggtcg taacaatgct catatccaaa tcttctataa
    15301 agtctatagt caataggcat caatatagaa actaattgcc cttttttata caattcgttt
    15361 aaagtaaatt tcattacttt gttcatatat ccagctcctc ttccctctgg aagtgtagaa
    15421 actcctacta catatgatgt attatatacc ttgctattta gtaataactt atactgattt
    15481 agctgaagag aagaaataat ttttccttca tctaaagcta ctacagtatt ttcagattta
    15541 tatttatcat taaaatagta cttcataaaa ctctccgtat cgttaaaaca ataactccaa
    15601 atctctttta tactttctat ctcttcttct ttagcatacc taatctccat atagtctatt
    15661 aagcctccct aacactgtac ttttcaacaa atctgcaagg atgataagaa agtttagctt
    15721 ttcttaatcc ttcaatacct aaatcttctt ctctatttac aaactcaacg tccttaaatt
    15781 cacttaccaa aaattgttga tttataaatg gatacagacc tcttatatcg ggattagctt
    15841 tttctatatg aatcaatgcc atatttggat ttaaaagctc tcctatacta aaagcttcta
    15901 attttccatc tacataaaca ccaaacactt ttaccttatc ttttaatatg tcataatggt
    15961 taaatatctt tttgatacct attaattcat catccatact ttcatcaaat tcattatttt
    16021 cttccttatt actttcccat tccttcatta gtactaaaca ctcatcaaag ttttctttat
    16081 ctaataattt agcttcatat ctacctgcat actcttttaa gaaatagttt atatggtttc
    16141 tcttcttttg attcttttta ccagctagag ttcttaaact ttctgcatca taaatatagt
    16201 caaataaatc tctttcttct atatactcaa atctacctgg atatttttct tttaaaaact
    16261 ctactacttc agtagtaata cctcttaagt atattttttt attattttta gaaaaatatt
    16321 caaggacaaa atctacaact tctggaagtt tatctttttt agcaagtggt aatattgaga
    16381 agctatctcc ttcgtattcc ccaactaaaa cggcaaaatc ttcacctata taatatcctg
    16441 tcttataaac atgttgccac atatataaag tactaaaaca gtactcacaa gcttcataat
    16501 ctactaaatc aaaatatggg tctaactctt tttttgaatt tagttcaata tctttgaaaa
    16561 acataaaatc ctcctacctt ttttattgct taattttaaa aattttattt tgtatatata
    16621 aaaaactcca attttattat ggtattaatt tcatttatat tgtttaaaat ataaaatata
    16681 ataagtcata tatatcttat attacccatc ataaatttat ataaacatgt aatgaatgga
    16741 gtgatttttt attgagaaat aaaatttttt tatcaatact tattataata tcacttttaa
    16801 tcggctgtga caaaaaatct ctactatcta tacatatgat tgatgttgga caaggtgata
    16861 gtattttagt tcaaacaccc acaaataaga atatattaat tgatggaggt gatgaagata
    16921 gcgaaaatat aattattagt tatcttagac aaaaaagaat aaaaactatt gatattataa
    16981 tagcaactca tccagattca gaccacatag gaagtcttga taatattata aaaaaattta
    17041 atgtaaactc catatatatg cctgaacaat caactgatag tgaggcttat caaaatctaa
    17101 taaattcttg tacggataaa aagttatcta ttcagcatct ctataaaaat gatgttttaa
    17161 acatagataa caatataaat atatatgtat taagtccatc atatatacaa gaagaatcta
    17221 atttaaattc tatagttttc aaattaactt ttaatgataa ttctttttta tttatgggtg
    17281 atgctgaaga agaaaatgaa aaagaaattt tacattcttt taaattaaat aatattaatt
    17341 ttataaaaat aggtcatcat ggaagtaatt cttcttcatc tttagaattt ataaaaaaaa
    17401 ttagtccaga tatagctgca atatcatgtg gatataaaaa tcaatatggt catccacata
    17461 gggaggttat taataatcta aaacaaaacc atgtatcaat ttatagaaca gacagaatag
    17521 gagatattgt tttttatagt gatggagaaa ttatatttac aaaatataat tatgaaatag
    17581 atacataatc taaaaaataa aactacaact acataaaata aggtaatata aaataactag
    17641 gctaaaagta tgaatataaa attgctttat attcatgctt ctagccttca aatttgtata
    17701 taaaatttat aaattaaaat atttaaaact tattatccta atagttcatt tactattaca
    17761 gatgcgatta ttaaacctcc tacagatggg acaaatgata cactaccagg tgttactttt
    17821 ctaccattca ttactttctt cttaagttct attggttgtt cagtagaata tactactttt
    17881 aactttttga tacctctatc cctaagttct ttcctcataa ctttagctag aggacaagta
    17941 cttgttttgt gtatatctgt aacgactatt ttagttgggt ctaatttatt tcccataccc
    18001 atagaactta ttattttcaa tccttttttt tcacaagttt caattagatg tattttggaa
    18061 cttaccatgt ctatcgcatc cacaacataa tcatatcttt cagttagtat ttcgtcacta
    18121 gtatctttat tataaagact tcttattttt tttatattga tattaggatt tatatctaat
    18181 attctttctt ccatgacatc aactttgtat cttcctacag ttgaatgtag ggctggtatt
    18241 tgtctattta tattagttat atctacatca tcaaaatcta caatagtaat attgcctact
    18301 ccagctcttg ctaaagcttc aacagtaaaa ctaccaactc ctccaactcc aaatactatt
    18361 atatttgaat tattaagttt ttctatccca tcatctccaa ctagaaaact agttctcgtt
    18421 gtaaaattat tttccatctt tccacactcc tagattttta tctaaatata taatagtatt
    18481 agaaaattta cattatataa tacaaaacat tatatattat taaaaataaa ttatcttaaa
    18541 ataaatttaa aaaggtatct tttaattaaa agataccttt tacatattgg cgggaatgcg
    18601 tgggaatcga acccacctaa gaggctctta acccctcata ctagttttga agactagagg
    18661 gcacaccggc acccatccac tcccataatg aaatccttct caacgaaatc tataatatca
    18721 tatattttga ctctttgcaa caatttttat agatttatat gatagtaaat tataatttaa
    18781 atagctttta tctatacatg gttaaaattt acctgtttta ttacatatcc aaaataaaaa
    18841 actggaaatt atatttttag aatctataaa gagtttcttc tctatataat caaaatataa
    18901 tttccagtaa aaatataaat tatttaacta ttttaatata cgtatatcta tagtttttct
    18961 tccccagtta taaactgttt ttgaatcatt catgtagata tcaattttat ttccttttat
    19021 tgctccacca cagtcttctg cagtaaacac catgtcaaat tgaggtatat aaactttagt
    19081 tccatatggt ataaccttag ggtctacagc tattgttccc cacttaggtt ttgttcctgt
    19141 tgcagttata ctgtgaccag tatatgcagt tgctaccact ctcatatttt taccattgtt
    19201 actactactg ctactagatg atgatgaagc cattcttctt gaactttctc cccttgatga
    19261 ggctacttgc atttctacct ttgtcccttt gcttacaacc ttatttactg gctcagcttt
    19321 aacaaccttt ttcacaaatt ccttttctac tttctttcca ttgtgataag tttctttata
    19381 tattagttca cttttaccag actttccttc ttctttaacc ttagaatcac cctttaataa
    19441 agagccatct tcaacaactg taacttcaaa tggaatgtct ttagtttcta caactgtttt
    19501 ttcctttaca tcaattactt ctattttatc tccattagat aactttgtat tttcatctaa
    19561 agttactata tcatttgcat catagctaat gttttgagat tctaaaaggt ctcctacatt
    19621 agaactaaat gttgatatag ttctttcttc tccccttact actaatgtta cttgtttatt
    19681 aagcgcagag tatcccgata atattccaac tgataaaaca actgccatta ccgagacttt
    19741 agcattactc aattttttaa aattcatata aaatttcctc cctaagattt tttacactat
    19801 atattttgct tcataaaatt ctgctttgta agtttctttt tttatttgtg ttttttgtgt
    19861 aactaaattg taactatttt ttcaagagtt gtcaacttga caaaaaaata gaaatatgca
    19921 tacatagtat tttcaacttt tcaagcatat ttctattagt tacaacaatg tcattgttta
    19981 ttgttgtttt gttatcattt tgttaccttt tacttttata tattttctat ctgccaatta
    20041 ataggttctt ttcctagctt tttcaaaatt tcatttatct tagagaagtg tttacatcca
    20101 aaaaagcctt tacttgctga caatgggctt ggatgaggtg cttccaaaat ataatgttta
    20161 cttgtatcaa tcaactctac ttttttacgt gcattacttc cccatagtac aaaaataact
    20221 gggtcttcac gctcacttaa aatagatatt actctatcag tgaaaatttc ccatcctttg
    20281 ttcttatgag aattagcttc atgcgctcta acagtaagag cagtatttaa aagtaacact
    20341 ccttggtctg cccaaggcat taaatatcca ttattaggta taaaacaacc taaatcatct
    20401 tttaattctt tatacatatt taatagagat ggtggagttt tcacttgtgg ttgaactgaa
    20461 aaagctaaac catgagcttg attatctcca tgataagggt cttgtcctaa tattaagact
    20521 tttgtgtctt tgtatgaagt gtgttttaaa gcttcatata tattgtgcat atttgggaaa
    20581 atttggcgtg ttttatattc ttctataaga aattttctta agtttaagta gtaatctttt
    20641 tcaaattctt cttttaataa ttcatcccaa tcgtttccta agttaaccat aattatcacc
    20701 aattcctttc aattattttt attattttat ttagaaatta tatcttcttg attatcattc
    20761 ttttcatttg tatttatttt tatgctcact ctattaggta tacaaactat gctttgactt
    20821 ggcttgttta taaatccagt atgtacgcaa accttatcag ggcatgaagc atctactatc
    20881 tcaacccctt tatcatgaat ctttactatg ttataccccc ctctattctc tattttaaat
    20941 tcttctttgg catttattga aacagtttta tacaatttat tatctacata aatctcaata
    21001 tttccagcat ttttagaatt tataacattt attatcccat aacatgctgt aataacaacc
    21061 aaaatgccta aaataagaag aatgtctttt ttcttcattt tttgtcctcc tatgaaatta
    21121 tgttataata aaataataac acaatttcat aggaggaata taccatgaat aagaaaaaaa
    21181 tcacatttat ttttgtgata ttgattttag cattaatttt attttcattt tttaggagtt
    21241 caagccagaa tactaaatat agtaaaacta gctactacct tggtacagta aacgaagtta
    21301 ctgtatttaa tgtaaaagaa tccaaaagtg ataaaatctt aaatgaatgt gattcaattt
    21361 taagatatat tgacaacaaa atgagcactc atataccagg aagtgatgtt tctaaaataa
    21421 atgataatgc tggtaaaaaa tttgtcagag tttctgatga tacatttttc gtcgtaaaag
    21481 aagctataca atattccaaa ttatcagatg gatattttga cataacaatt ggtccattat
    21541 caaatttatg ggctattgga acagataaag ctaaagttcc aagtgattct gagatacaaa
    21601 aacttctgcc tcttattgat tataagaaca tcatattgga tgaaaaaaat aaatctataa
    21661 aattaaccaa agaaaatatg aaaattgatt taggtgccat agctaaagga tatgctgctg
    21721 ataaaattgt agcctattta aaatcagagg acgtaaaaaa aggtttagta aacttaggag
    21781 gaaatatatt tactttagaa aatggtaaaa atgataaacc ttttaaaata ggtattcaag
    21841 accccactag taaaaatggt gaatccatag gaaacataga aaccacaaat aaatctgttg
    21901 ttacttctgg aatttatgaa agatttatcg aaaaaaacgg aaaaatatat catcatatgt
    21961 taaatccatt tacagggtat ccttttgaaa acaatttaag tagtgttaca ataatctctg
    22021 ataaatctat taattgtgat gccttatcca cttctacatt tggacttggt cttgaaaaag
    22081 gtatggattt aattaataaa attgataatg tagatgctat attcataact aaggataaga
    22141 aagtatacct aactaagggt ataaaagata attttaaact tacagataaa tctttttcta
    22201 ttgaaaaatt aaataaa
//
LOCUS       DAEMIK010000062        22183 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEMIK010000062 DAEMIK010000000
VERSION     DAEMIK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.062"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..237
                     /gene="tuf"
                     /locus_tag="KPK87_001951"
     CDS             <1..237
                     /gene="tuf"
                     /locus_tag="KPK87_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG2538101.1"
                     /translation="GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            608..919
                     /gene="rpsJ"
                     /locus_tag="KPK87_001952"
     CDS             608..919
                     /gene="rpsJ"
                     /locus_tag="KPK87_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG2538102.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1011..1640
                     /gene="rplC"
                     /locus_tag="KPK87_001953"
     CDS             1011..1640
                     /gene="rplC"
                     /locus_tag="KPK87_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG2538103.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1670..2293
                     /gene="rplD"
                     /locus_tag="KPK87_001954"
     CDS             1670..2293
                     /gene="rplD"
                     /locus_tag="KPK87_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG2538104.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2293..2583
                     /gene="rplW"
                     /locus_tag="KPK87_001955"
     CDS             2293..2583
                     /gene="rplW"
                     /locus_tag="KPK87_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG2538105.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2613..3443
                     /gene="rplB"
                     /locus_tag="KPK87_001956"
     CDS             2613..3443
                     /gene="rplB"
                     /locus_tag="KPK87_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG2538106.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3478..3759
                     /gene="rpsS"
                     /locus_tag="KPK87_001957"
     CDS             3478..3759
                     /gene="rpsS"
                     /locus_tag="KPK87_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG2538107.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3791..4126
                     /gene="rplV"
                     /locus_tag="KPK87_001958"
     CDS             3791..4126
                     /gene="rplV"
                     /locus_tag="KPK87_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG2538108.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4149..4964
                     /gene="rpsC"
                     /locus_tag="KPK87_001959"
     CDS             4149..4964
                     /gene="rpsC"
                     /locus_tag="KPK87_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG2538109.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5000..5431
                     /gene="rplP"
                     /locus_tag="KPK87_001960"
     CDS             5000..5431
                     /gene="rplP"
                     /locus_tag="KPK87_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG2538110.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5433..5636
                     /gene="rpmC"
                     /locus_tag="KPK87_001961"
     CDS             5433..5636
                     /gene="rpmC"
                     /locus_tag="KPK87_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG2538111.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5660..5914
                     /gene="rpsQ"
                     /locus_tag="KPK87_001962"
     CDS             5660..5914
                     /gene="rpsQ"
                     /locus_tag="KPK87_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG2538112.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5940..6308
                     /gene="rplN"
                     /locus_tag="KPK87_001963"
     CDS             5940..6308
                     /gene="rplN"
                     /locus_tag="KPK87_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG2538113.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6332..6637
                     /gene="rplX"
                     /locus_tag="KPK87_001964"
     CDS             6332..6637
                     /gene="rplX"
                     /locus_tag="KPK87_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG2538114.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6667..7209
                     /gene="rplE"
                     /locus_tag="KPK87_001965"
     CDS             6667..7209
                     /gene="rplE"
                     /locus_tag="KPK87_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG2538115.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7227..7412
                     /locus_tag="KPK87_001966"
     CDS             7227..7412
                     /locus_tag="KPK87_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG2538116.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7445..7843
                     /gene="rpsH"
                     /locus_tag="KPK87_001967"
     CDS             7445..7843
                     /gene="rpsH"
                     /locus_tag="KPK87_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG2538117.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7874..8416
                     /gene="rplF"
                     /locus_tag="KPK87_001968"
     CDS             7874..8416
                     /gene="rplF"
                     /locus_tag="KPK87_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG2538118.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8452..8820
                     /gene="rplR"
                     /locus_tag="KPK87_001969"
     CDS             8452..8820
                     /gene="rplR"
                     /locus_tag="KPK87_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG2538119.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8841..9350
                     /gene="rpsE"
                     /locus_tag="KPK87_001970"
     CDS             8841..9350
                     /gene="rpsE"
                     /locus_tag="KPK87_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG2538120.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9365..9550
                     /gene="rpmD"
                     /locus_tag="KPK87_001971"
     CDS             9365..9550
                     /gene="rpmD"
                     /locus_tag="KPK87_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG2538121.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9583..10026
                     /gene="rplO"
                     /locus_tag="KPK87_001972"
     CDS             9583..10026
                     /gene="rplO"
                     /locus_tag="KPK87_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG2538122.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10070..11338
                     /gene="secY"
                     /locus_tag="KPK87_001973"
     CDS             10070..11338
                     /gene="secY"
                     /locus_tag="KPK87_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG2538123.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11355..12005
                     /locus_tag="KPK87_001974"
     CDS             11355..12005
                     /locus_tag="KPK87_001974"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG2538124.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12006..12752
                     /gene="map"
                     /locus_tag="KPK87_001975"
     CDS             12006..12752
                     /gene="map"
                     /locus_tag="KPK87_001975"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG2538125.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12774..13043
                     /locus_tag="KPK87_001976"
     CDS             12774..13043
                     /locus_tag="KPK87_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG2538126.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13081..13299
                     /gene="infA"
                     /locus_tag="KPK87_001977"
     CDS             13081..13299
                     /gene="infA"
                     /locus_tag="KPK87_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG2538127.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13335..13448
                     /gene="rpmJ"
                     /locus_tag="KPK87_001978"
     CDS             13335..13448
                     /gene="rpmJ"
                     /locus_tag="KPK87_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG2538128.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13601..13972
                     /gene="rpsM"
                     /locus_tag="KPK87_001979"
     CDS             13601..13972
                     /gene="rpsM"
                     /locus_tag="KPK87_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG2538129.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14008..14406
                     /gene="rpsK"
                     /locus_tag="KPK87_001980"
     CDS             14008..14406
                     /gene="rpsK"
                     /locus_tag="KPK87_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG2538130.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14438..15061
                     /gene="rpsD"
                     /locus_tag="KPK87_001981"
     CDS             14438..15061
                     /gene="rpsD"
                     /locus_tag="KPK87_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG2538131.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15139..16086
                     /locus_tag="KPK87_001982"
     CDS             15139..16086
                     /locus_tag="KPK87_001982"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG2538132.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16107..16448
                     /gene="rplQ"
                     /locus_tag="KPK87_001983"
     CDS             16107..16448
                     /gene="rplQ"
                     /locus_tag="KPK87_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG2538133.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16568..17401
                     /locus_tag="KPK87_001984"
     CDS             16568..17401
                     /locus_tag="KPK87_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG2538134.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17389..18255
                     /locus_tag="KPK87_001985"
     CDS             17389..18255
                     /locus_tag="KPK87_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG2538135.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18249..19052
                     /locus_tag="KPK87_001986"
     CDS             18249..19052
                     /locus_tag="KPK87_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG2538136.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19084..19815
                     /gene="truA"
                     /locus_tag="KPK87_001987"
     CDS             19084..19815
                     /gene="truA"
                     /locus_tag="KPK87_001987"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG2538137.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19935..20366
                     /gene="rplM"
                     /locus_tag="KPK87_001988"
     CDS             19935..20366
                     /gene="rplM"
                     /locus_tag="KPK87_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG2538138.1"
                     /translation="MKSYIAKPTDVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20395..20787
                     /gene="rpsI"
                     /locus_tag="KPK87_001989"
     CDS             20395..20787
                     /gene="rpsI"
                     /locus_tag="KPK87_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG2538139.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21246..21998
                     /gene="cwlD"
                     /locus_tag="KPK87_001990"
     CDS             21246..21998
                     /gene="cwlD"
                     /locus_tag="KPK87_001990"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG2538140.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8912 a         2593 c         4550 g         6128 t
ORIGIN      
        1 ggtggaagac atacaccatt ctttgatgga tatagaccac aattctattt cagaacaaca
       61 gacgtaacag gagcttgtaa gttaccagaa ggaatagaga tggtaatgcc tggagataac
      121 gtaacaatgg aagtagactt aataaactca atagttgtag aagagggatt aagattctca
      181 ataagagaag gtggaagaac agtagcttca ggagttgttg ctacaataat agagtaatcc
      241 tttactaaac taatggattt aatataaaaa aagagggggg taactcctct cttttttgtt
      301 ttttgttgac aagtagcgct aaagcatata tactaaacta gtgccttgag acagattaac
      361 aatcaagttt aaggttatga tgttgtaata gaaaataaaa ttgaaaaaaa cagaaaaaaa
      421 cttgaaaaaa tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat
      481 aataattaaa tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg
      541 agcagtgtat cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga
      601 aaacaaaatg gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt
      661 attagatttt tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc
      721 aggacctgtg cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa
      781 gtacaaagac tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc
      841 taatccaaca cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga
      901 tatagaaata aaattataaa aacccctaag atattatcct atagatgatt atcttagaat
      961 gattactcta aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa
     1021 tattaggcaa aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg
     1081 taacagctgt tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg
     1141 gatacaatgc tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta
     1201 aaaaaggaca tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag
     1261 attctgtaga aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg
     1321 caaaaataga tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac
     1381 atggacaaag tagaggtcct gaaactcacg gttctagata ccacagaaga ccaggttcaa
     1441 tgggagcttg ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg
     1501 gaagcgtaaa ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc
     1561 ttatattagt taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag
     1621 ctataaaggt ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt
     1681 aaatgtacta aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt
     1741 tggcgtagaa gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa
     1801 gagacaagga actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc
     1861 ttggaaacaa aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt
     1921 aggtggagga gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa
     1981 ggtaagaaga ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat
     2041 agtattagat gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa
     2101 caatataaat gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat
     2161 aaaatcagct agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata
     2221 tgatatatta aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga
     2281 ggtgtacgca taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac
     2341 agtatggctg aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa
     2401 actgaaataa aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact
     2461 ttaaactacg atggaaaagt taaaagaatg ggtagaactc aaggaagaac atcaagcttt
     2521 aagaaagcag tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg
     2581 tagtattata acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt
     2641 ctcctgcctt aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag
     2701 aaaaatctct tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa
     2761 ctgttcgtca tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag
     2821 ataaagatgg tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa
     2881 atatcgcatt attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa
     2941 ttaatgttgg agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt
     3001 tagcattaaa agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa
     3061 aaggagctca attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa
     3121 aaaatgcttt attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag
     3181 ctactatagg tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta
     3241 gaaaaagaca tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc
     3301 atccacacgg tggaggggaa ggtagatctc caataggtag accatctcct gttactccat
     3361 ggggtaaacc agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa
     3421 tagtatcaag aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg
     3481 tcaagatcaa ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct
     3541 atgaatgcta gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt
     3601 ccacaaatgg tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat
     3661 ataacagaag atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa
     3721 ggacataagg atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg
     3781 aggaataaca atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc
     3841 aggccaaata tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa
     3901 gtttactcca agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa
     3961 cgcagaaaac aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa
     4021 tcaaggacca acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag
     4081 aaaaagaact tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag
     4141 ggaaagtaat gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt
     4201 gggactcaag atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc
     4261 ataatatacg taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa
     4321 tagaaagatc agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta
     4381 taggaagagc tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga
     4441 aaactataat agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag
     4501 ctgaaaatat agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag
     4561 ctatacaaag agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat
     4621 taggtggagc tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa
     4681 ctttaagagc agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg
     4741 gtataaaagt ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa
     4801 gagaagaaag cagaaagagc gatagaagag acaacaagag agataataga agaaacgata
     4861 gaagaggaaa tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc
     4921 aaggtggatc aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa
     4981 ggaaggagga aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag
     5041 aggaagcatg gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt
     5101 agtagcatta gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat
     5161 gactagatat ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt
     5221 aacaagaaaa ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt
     5281 agctgtagtt aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc
     5341 aagagaagct atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa
     5401 agaagattta gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa
     5461 caagcgaaga gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat
     5521 tccaattagc tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata
     5581 tagcaaaagt taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg
     5641 gaaaggaggc tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt
     5701 tagtgataag atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact
     5761 atataataaa cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg
     5821 taacatcgga gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt
     5881 cagactagtt gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata
     5941 tgatacaaca agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat
     6001 gtatacgtgt tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg
     6061 ctactgttaa aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg
     6121 ttatagtaag aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg
     6181 aaaatgctgc tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc
     6241 ctgttgctag agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag
     6301 tactataatt aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt
     6361 agttatagca ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac
     6421 tagcaaagtg ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat
     6481 gaaccaacaa ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc
     6541 atttgatcct gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa
     6601 agtaagagta tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga
     6661 ccttaaatgg cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg
     6721 gagaaatttg gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac
     6781 atgggtatag gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta
     6841 gaaatgatat caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc
     6901 aaattaagag aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc
     6961 tacttcatgg acaaattagt ttcagtttct ttaccaagag ttagagactt tagaggagtt
     7021 aaccctaatg cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata
     7081 ttccctgaaa tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt
     7141 actacagcaa aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt
     7201 tctaagtaag caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa
     7261 gaaagcaaaa gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt
     7321 cagttttgaa aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc
     7381 aaatacctgg tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa
     7441 caaaatgaca atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt
     7501 tgttaagcat gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat
     7561 cttattagaa gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat
     7621 cataagaata caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa
     7681 aatatctaag cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa
     7741 cggattagga atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag
     7801 aaaagaaaat gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta
     7861 aggaggtgcg accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga
     7921 agttactata gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa
     7981 acaattaagt gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc
     8041 aactgataat aaaaaacata gatctttaca cggactaact agaactttat tagataatat
     8101 ggttgtaggt gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag
     8161 agctcaaaaa caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat
     8221 ggaagatcca gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg
     8281 tatagacaag caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga
     8341 gccatacaaa ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa
     8401 aactggtaaa aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa
     8461 aaggctgata aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc
     8521 ggtacttctc aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa
     8581 ataatagatg acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt
     8641 aagagtgctg tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt
     8701 gctaaaaaag ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta
     8761 tatcatggaa gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa
     8821 tacgaaggag ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact
     8881 tacaagaaaa agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt
     8941 tcagatttgc agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg
     9001 ggaaagcaat ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc
     9061 taatagtagt gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg
     9121 ctggaaatat attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg
     9181 ctagagccgt acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta
     9241 acaatccaag aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg
     9301 aagatatagc taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg
     9361 taaaatggct aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca
     9421 aaaaaagaat gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga
     9481 caatgctcaa acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat
     9541 agctgaataa tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta
     9601 aaacctgctg aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga
     9661 caaggtaaaa ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta
     9721 agagtaggat ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc
     9781 aataacatat ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa
     9841 aatggaacag aaataactgc agaattatta aaatctacta aaactataag caaaataggt
     9901 aaagacggaa ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct
     9961 gctaaattta cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta
    10021 gtataataac ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa
    10081 attaaaacaa gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat
    10141 gatcgtgata ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat
    10201 acaaaagatg gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc
    10261 atttagtaac ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat
    10321 aatacaactt ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga
    10381 aggaaagaaa aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca
    10441 agctttggga attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt
    10501 tatcacaact gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga
    10561 caaaattact gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat
    10621 atcaagaata cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc
    10681 accttgggtt atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt
    10741 tatacaagaa gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa
    10801 aatgtatgga ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc
    10861 tataattttt gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc
    10921 aaatgctcag gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac
    10981 atatagatca attgagattc tacttataat tttcttctca tatttttata caactgtatc
    11041 atttaataca gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat
    11101 aagaccaggt gagcctacta tggattattt aaatagaatt ttaactagat taactttagc
    11161 tggagctact ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa
    11221 agtaaatatg agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct
    11281 taagagacag ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa
    11341 tggagatgga taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc
    11401 aggcggcagg aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca
    11461 gaaagaatat aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag
    11521 gtttattagt accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag
    11581 attgtaaaaa tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt
    11641 tagatatctt cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg
    11701 ataagagtat attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta
    11761 cttaccatgt tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag
    11821 aattatatca aagagctgat gataatgaag aaactgtatc taagagaata caagtttatt
    11881 tagatgaaac taagccttta gtagattatt atagcaaaca aggtataata gcagatataa
    11941 aaggtgatca agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa
    12001 aataaatgat tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa
    12061 tagttgctga cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag
    12121 aattagataa aatagctgaa gagaatataa gaaaatacaa tgcagaacct tcttttaaag
    12181 gatatggagg ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta
    12241 ttccaggaga aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt
    12301 ataaaggata tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag
    12361 ataggaaatt gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat
    12421 taggatatag actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt
    12481 tctcggtagt tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac
    12541 aagtacctaa ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg
    12601 ctatagagcc aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga
    12661 cgactgttac aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg
    12721 aacatgaacc tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat
    12781 cagataattt aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt
    12841 tattctttgt agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga
    12901 gaaaacttga caaacctaag ttaaagaaag tgaagcactt aaaaaaatat aattttatta
    12961 atgatgaagt tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag
    13021 ctgaacttac taagttaaat tagataattt aacttattga aatggagggc tggttagtta
    13081 atggccaaaa aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct
    13141 atgttcaaag ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta
    13201 agaatgaact tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat
    13261 gaccttacaa gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta
    13321 aggagggatt aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag
    13381 taataaaaag aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc
    13441 aaggataaga aattattact agcattttgt taaattttat agtataatac tatattgtga
    13501 tgttaacaaa agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg
    13561 aagttttaaa cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt
    13621 agatttacct agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa
    13681 agcaactgct aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga
    13741 tttatcagaa gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga
    13801 aggtgactta agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta
    13861 tagaggaata agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc
    13921 tagaactaga aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa
    13981 taactagatg aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt
    14041 agaagaagag aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat
    14101 aatacaataa taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga
    14161 caattaggat tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa
    14221 acagctgcaa aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg
    14281 ccaggttcag gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact
    14341 atgataaaag acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga
    14401 gtgtaattaa taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc
    14461 atgtagacaa tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac
    14521 agacaaatgt gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa
    14581 gaaagtttct aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg
    14641 agttttagag actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc
    14701 aggtgagaac ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg
    14761 actagcatct tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa
    14821 tggaaataaa gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa
    14881 agagaaatca agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc
    14941 tcctaagtgg ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac
    15001 aagagaagat atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata
    15061 ataagcacac ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt
    15121 ttaaggaggg ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt
    15181 agcgaagact atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata
    15241 actataggta acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat
    15301 gctataaaaa tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat
    15361 gttactgaga taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt
    15421 agaacactta aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt
    15481 cctccagatg ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct
    15541 aaacttaata tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat
    15601 aaaacagaga atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag
    15661 aaggttagtt atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta
    15721 gtactagaag tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca
    15781 aaggtattag ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt
    15841 gaaatcatgg tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa
    15901 gaattagatt tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt
    15961 gaagaattag ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag
    16021 tcattagaag aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa
    16081 gaatagcgag caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag
    16141 ctcacagaaa ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag
    16201 aaactacagt gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg
    16261 ctaagagagg agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag
    16321 tagttaataa cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca
    16381 ctagaataat aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat
    16441 tagtatagta aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta
    16501 aggtatgata taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta
    16561 tgagttaatg gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga
    16621 agcaaaactt aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc
    16681 gataatagga cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct
    16741 tatgcctact gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt
    16801 atgggatata agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc
    16861 tactatagta gaagaagatg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga
    16921 aataagacga attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag
    16981 acagcctcat ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc
    17041 tatgaggcct aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag
    17101 aaaagaagtt atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca
    17161 tataacgcac tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg
    17221 aaagaaaatc ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga
    17281 aatcggttta gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat
    17341 aaatattagt agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata
    17401 gtaaagaatt taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat
    17461 gatgtttctt ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca
    17521 ggaaaatcta cattaataca acatttaaat ggattactaa agccttcctc aggagaaata
    17581 tttataaatg attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga
    17641 gtaggggttg tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat
    17701 atagcttttg gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa
    17761 gcatcaatgg aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa
    17821 ttatctgggg gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa
    17881 gttctcatat tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt
    17941 aacttaataa aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt
    18001 atggatgata tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa
    18061 tttatgggaa ctcctagaga agtatttaaa tcaaatgcta gtaaattaaa agatatagga
    18121 ttagatattc cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc
    18181 agtgaggata tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga
    18241 cgaggattat gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc
    18301 ataaattaga tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg
    18361 tagtaaataa attttggcct tatatagtag ttttattatg tttattagct atgataaaat
    18421 tggctaatat accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt
    18481 tgtttacatt tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat
    18541 ttttagctat tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat
    18601 ttttagtagt tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg
    18661 gaatagagag attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa
    18721 tgatgatgac aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa
    18781 tgaaggctca gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa
    18841 agaatttagt accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat
    18901 tggctatggc catggaggcc agatgttaca gaggtggaca taataggact aagatgagag
    18961 agtctgttat atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag
    19021 caattatagc tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa
    19081 agtatgagaa atataaaaat taaaatacaa tacaatggta agaattattg tggatggcaa
    19141 aagcaacctg attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca
    19201 aaagaagaaa ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt
    19261 cagattgcaa attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta
    19321 aatgccaagt tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt
    19381 cactccagat acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt
    19441 agaaatccaa tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa
    19501 atgtgttcag aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct
    19561 ggttcatctg taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat
    19621 gacttgatta cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt
    19681 gttggaacac ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata
    19741 caatcaaaaa ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa
    19801 aaagttcatt attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt
    19861 aataagtcca cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt
    19921 aaggagggac agttatgaaa agttatatag ctaagccaac tgatgtacaa agaaaatggt
    19981 acttagttga tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat
    20041 tgagaggtaa acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg
    20101 tagtaaatgc agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat
    20161 accatactgg ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga
    20221 aaccagaaga agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa
    20281 gcagaatgat gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa
    20341 atccagaagt tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct
    20401 aacgttcaat attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt
    20461 gcaggtgaag gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag
    20521 acattaataa gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat
    20581 gttatagtaa aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga
    20641 atatctagag ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt
    20701 ttcttaacta gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga
    20761 agagctccac aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta
    20821 tttactgtta ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat
    20881 atataataaa ataataagat atcttattag gatgtttttc attaagaaga acatcctttt
    20941 tattttgact atttaataaa aaacatatgc taataacatt taaattttat ttttataata
    21001 ttgtaattta aaagtttata taaggttata tattttaaga tattatttaa caatataaat
    21061 tctgaatata cgttatcaaa atttataata taaactaagt ataatttatt gtatagtatt
    21121 gaatgttaga taaacatagt gaattaaata gggacagaaa tataatatca ttacataata
    21181 atatattaat accaataaat aaagatatat caatattaaa aaaatttaaa tgtttgtact
    21241 aatatatgga atattatagt gtttttataa gtatacatat acagggtgat gttgtgagaa
    21301 agtacataaa acatataatt tttagttttg caatgatatg tctagtagta gtatccatat
    21361 ttgaaattaa aaatatttct gaagatgtta tcaagtatat gccagtaaca aacaaaacta
    21421 taattttaga tgctggtcat ggaggcattg atccaggtgc attaaataag gataagagta
    21481 catctgaaaa ggatattaac ctagcaataa cacttaagct tagagagctt atagaatcaa
    21541 gtggtggcct tgtaatatta actcgagaag atgacagtag cctttataaa gaagaaaata
    21601 ataaaactac aagacaaaaa tataatgaga atttgaaaaa tagaaaagaa ataatatcta
    21661 attctaatgc caatatgttt gtctctatac atcttaatgc atttgaacaa tcaaaatact
    21721 atggagctca aactttttat cctaaagaca aacaagacag taaagaatta tcgaagtgta
    21781 ttcaagaaga acttaaaaga gtagtagata agacaaacaa cagagaagtc aaaccaaggg
    21841 atgatatata tcttttgaaa gaaaataata ttccatcagt actgatagaa tgtggttttt
    21901 tatcaaatga aaaagagtgt aaactcttaa ctgatgaaac atatcaagaa aaaatagcat
    21961 gggcaatcta cataggaata caaaaatatt taagttgaaa taactataaa aataggtcta
    22021 aatatgtaaa tagcttgtat taaagcagtt aaaatgcatt aatataggct atttttattt
    22081 tgacaaaaaa atatttaaaa taaaagttaa aaagttgttg acttagaata atatagatga
    22141 tattatatat gagtgcccaa aaggagcacc aaaataagac aaa
//
LOCUS       DAEMIK010000063        22052 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEMIK010000063 DAEMIK010000000
VERSION     DAEMIK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.063"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            445..1515
                     /locus_tag="KPK87_001991"
     CDS             445..1515
                     /locus_tag="KPK87_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538141.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1499..2368
                     /locus_tag="KPK87_001992"
     CDS             1499..2368
                     /locus_tag="KPK87_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG2538142.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2412..3272
                     /locus_tag="KPK87_001993"
     CDS             2412..3272
                     /locus_tag="KPK87_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG2538143.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3303..4607
                     /locus_tag="KPK87_001994"
     CDS             3303..4607
                     /locus_tag="KPK87_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2538144.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4670..5932
                     /locus_tag="KPK87_001995"
     CDS             4670..5932
                     /locus_tag="KPK87_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG2538145.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6119..8089
                     /gene="uvrB"
                     /locus_tag="KPK87_001996"
     CDS             6119..8089
                     /gene="uvrB"
                     /locus_tag="KPK87_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG2538146.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8108..10933
                     /gene="uvrA"
                     /locus_tag="KPK87_001997"
     CDS             8108..10933
                     /gene="uvrA"
                     /locus_tag="KPK87_001997"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2538147.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11192..13009
                     /gene="uvrC"
                     /locus_tag="KPK87_001998"
     CDS             11192..13009
                     /gene="uvrC"
                     /locus_tag="KPK87_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG2538148.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13009..13938
                     /locus_tag="KPK87_001999"
     CDS             13009..13938
                     /locus_tag="KPK87_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG2538149.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14152..15948
                     /locus_tag="KPK87_002000"
     CDS             14152..15948
                     /locus_tag="KPK87_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG2538150.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKDNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDIEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16088..17869)
                     /locus_tag="KPK87_002001"
     CDS             complement(16088..17869)
                     /locus_tag="KPK87_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG2538151.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17892..19778)
                     /locus_tag="KPK87_002002"
     CDS             complement(17892..19778)
                     /locus_tag="KPK87_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG2538152.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19795..20292)
                     /locus_tag="KPK87_002003"
     CDS             complement(19795..20292)
                     /locus_tag="KPK87_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG2538153.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20610..22052)
                     /gene="cls"
                     /locus_tag="KPK87_002004"
     CDS             complement(20610..22052)
                     /gene="cls"
                     /locus_tag="KPK87_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG2538154.1"
                     /translation="MAVTTIYIINFMVILNLIFREKRNINTTLTWLLILVLIPALGFI
                     LYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSDIHQHKDMIYALAN
                     SNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKDDKIGTEIIDILID
                     KAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKIVNFNLNYRNHRKI
                     VVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLNMRFILDWRYTTKE
                     DLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLKMIQKARKYIYIQS
                     PYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAGELLKFGAKIYTYG
                     QNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSRKALEQRIIFENDILD
                     SKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8476 a         2952 c         3697 g         6927 t
ORIGIN      
        1 gggtaaattt ataaaaatta aaaaagtttt attttttatg tttaaacctt gtaaatttgg
       61 gacataatat aattgtaaaa cttaatatag caaaaataaa ttactcaatc tcccccactt
      121 aaagacctct atcccccata gaggtctttt ttactttcaa atcaaattat ataaaaaata
      181 aatagtttta tatgactctt cataaattaa aattacattg tgatagcaaa ccatgaatat
      241 gaaaaatata gaaaattata gctaacataa gctgtatatc atcttaacct cagtatattt
      301 aacaaaaaat gtttataatt taacctttat tttaataagt ttaccatata ttcactttgt
      361 cttaatatat cctttacaaa ttaagagtat tattaatcta taagtaaatc gtagaattaa
      421 attaaatatt ttgggaggat aaaaatggca gatataagac tagaaaatgt aacaaaatct
      481 tataataaaa aaaatacagt tatagaaaat ttaaatctta ctatagaaga tggttctttt
      541 acagtattgg taggcccaag tggatgtggt aaatctacta cgttaagaat gattgcaggt
      601 cttgaagata taacttcagg taggttgaag attgaagaaa atgatgttac aaaaacggat
      661 gctagtaaaa gggatatagc tatggtattt caaaattatg cattatatcc acatatgact
      721 gttagagaaa acatagaatt tggtcttaaa aacaaaaaag tagaaaaaga aaaaagagaa
      781 aaacttataa cagaagttat tgatgtagta ggattgagag aatatttaaa tgtgaaacca
      841 ggcaacttat ctggaggtca aagacaaaga gtggcacttg ctagagctat ggttaaaaat
      901 ccaaaggtat ttttaatgga tgaaccattg tcaaatttag atgctaaatt aagaaatcaa
      961 atgagaacag aattaataca attacatcaa aaactaaagt ctacatttgt ttatgtaact
     1021 catgaccagg ttgaggcaat gtcgatggca gataagatag ttataatgaa caaaggaaag
     1081 ataatgcaaa taggttctcc aaagaagata tatcaaaatc ctaaaaattt atttgtagct
     1141 caatttatag ggagtccttg tatgaacata ctagatataa aaaatgatat taaatttgga
     1201 ttcagaccag aaaaagcaac actagagagt ataaacatag atgggaaaaa tgatgatgta
     1261 tttagtctat ttggaaaagt tgtaactaaa gaagtgttgg gaaatgaaac tatatattgt
     1321 ttatctgttc ttaatagaga gataagagtc aaaacggaaa atgatatatt tgatattgga
     1381 agaactttaa gaataagtgt attagaaaaa gatttatatt attttgataa aaatggagaa
     1441 agaatagaag atgtaaaagt atgtaaaagt atgtattcta aaattaaagg agatatttat
     1501 gaaaaagcaa tttaataaag catatttata tataacacct gctattatag gaactttatt
     1561 gtttatagtc tatccaataa ttaagacgat atctctaagc ttcaaatctg gtagtcttct
     1621 tagttcaacc tttgaaaatg taggcttaaa caattataca gaacttttaa agaatccaga
     1681 gtttatacaa actataacga acactgcaat ttatactttt tttatggtga ctatatctat
     1741 atcactagcc atagtacttg ctgtttggct aaataaaaat tctataatac acaatttaac
     1801 gcaaagtata atctttacac cacatgtaat ttcactagta tctgtagctg tattgtggct
     1861 ttggattatg gagccacaat atggcttttt aaactggata ctatcgacat tacatttacc
     1921 aacatcaaag tggctttcaa gtgaaagttc agctttaatg tcactaattc taataggaat
     1981 atggaagact cttggatata atatcttaat agtgttatca ggtcttaaat ccataccaaa
     2041 ctatatatat gaagcatcta aattagataa tgcaaacaaa ataacgaatt tctttaaagt
     2101 gacattgcca ctattatcac caacaatatt ttttcttatg ataacaacaa caactgcatc
     2161 ttttcaagta tttgatgaga taagtataat gacacaaggt gggccagtgg gttcaactaa
     2221 catgttatct tattatatat atgagtctgg atttatatac tatgacatag gacaagcgag
     2281 tgcagcgagt atgtttttgt tagctatagt tatgattgct acatatgcta actttagact
     2341 cttatcaaag aaagttcatt atcaataaag aaaaagtaga tattataagg tttatccaaa
     2401 aggaggagac aatggatatt atacaaccta tcaaaaaaga caaagttgat ataaaaaaaa
     2461 tagcatcaaa atcaatagaa atattaattc tattactttt atctgcaata tttatattcc
     2521 cttttatatg gatgctatct acatccttta aaaatccaca agaaatgatg caattaccgc
     2581 ccactattat accaaatgat atagtgttag aaaattatag tacagcatgg cattcaggtc
     2641 cattttttag atatttgcta aacagtgtat ttgtaacagg aagtgtttta attgtacaat
     2701 ttttagttat ggttccagca gcatatgcat tttctaagat taaatttaaa ggtgaaaaaa
     2761 tgctatttgg gcttttgctt gtaggactta tgattccacc acaagtaact tttctaccag
     2821 tctatatgat gatgagtaag tttggattta taaatactta tgttccactt ataattccat
     2881 ttatgacatc ttcatttggg atatttctgc ttagacagaa ttttatgcag atttcagatg
     2941 aaattattga agcagctaaa ctagataatg cttctgactt aaaaataatg tttagaataa
     3001 tggttcctat ggcaaaacca gcagtaataa catttatatt atttaatttt atataccatt
     3061 ggaataatta tttctggccc ttagtaatga caaatacaga tgcaataaga actttaccaa
     3121 taggagttgc gctccttaaa tcttcagaag gaattactgc atggaatgta ataatggcag
     3181 gaaatataat tctaatttta ccaataattc ttgtttatat atttgcgaat aaaaaagtaa
     3241 aagaagcttt tatgtattca ggaataaaat aaaaaattaa agactaaatt tggaggatga
     3301 aaatgaagct taaaagaaaa ttagcaacag gattagcaat aacattatta ttaggttctt
     3361 tagttgggtg ttcaaattct aatgatgcaa atgcatctaa aggagaaaag actgatagca
     3421 atattacgaa gatagatttc tgggctcctt taggtggaac taatggagaa actgctcaaa
     3481 aaatggttga ccaatttaat agtgaacata aggatgtaca agtaaatatg ttaaaacaga
     3541 aagattacta tgaaaatgca acaaaactac aggcagcctt aacatctaaa gaccaaccag
     3601 atgtaacatt attagaaata actcaaacag gtacattcgc atcagcaggt gctttagtag
     3661 atatgagtaa atattttgat aaaacatatc aagaaagatt cttttctggt cttttaacaa
     3721 attcatatta tgaggataag tttgttggta tgccatttaa tagaagtaca ccaatattat
     3781 acataaataa agatatggca actaaagcag gtcttgaccc ttcaggtcca aaatcatggg
     3841 aagagcttaa ggattatgct agtaaaatga caaataaatc agaagatact tatggttttg
     3901 aaacgccaat agatatttgg ttctatgaag ctatggtaat gcaaagtgga ggagaaataa
     3961 ctaatggtaa aaaagtagca tttaataatg aggcaggaca agcaccagtt aagttctggc
     4021 aagatatgat gaaagctgga ataatgaaaa tgcctcctgg agaagattat aatgcctggg
     4081 atgtagcaaa acaagatttt gtaaatggta aagtaggtat gatatttaca tcaacagcag
     4141 atttagcagg acttatgcaa caaacagaag gtaagtttga aatatcaaca gcattcttac
     4201 caaaaaatca aaaatacgca actcctacag gtggagctaa tctagttatg cttgaaggtg
     4261 gaacagataa agaaaaagaa gcaagtgcag aatttataga atggatgaca caaacagata
     4321 aaatcgttca gtttagttca tcaacaggat acttaccaac aactgaagat gcaacaaact
     4381 cagaaaagtt acaaacatta tataaagaaa aacctcaata taaggttgca actgaccaat
     4441 tacaatatgc tgttgcactt ccaatgttaa atggatacaa ggaagcaact gataagctaa
     4501 tggatgagat taaaaagggg cttactgatt taaatgcaaa tccaaaagat gtagtaagca
     4561 aaggaacttc tgcaatgcaa gcagtaatag ataaaacaaa taaataatta tatagacttg
     4621 taagttgatt aaaagtattc aataatatat aaatgaagag gtaatgtaaa tgacaaaggt
     4681 tatagtatta tcagtagatt ctttatttga aaaagacttg gaatttgtaa aaaatctacc
     4741 aaactttaaa tctatactag aaaattgctc catagtaaaa aatataaatt gtgtttatcc
     4801 cactctgaca tatccgtgtc atactagtat ggttacagga gtatatccta aaaaacatgg
     4861 tatatatcat aatgaaaaat ttgacccaaa taaagaaaat aaagactggt actggtattc
     4921 aaaggacata aaagcaaaaa ctattataga tgtagcaaaa gaaaataatc taactacctc
     4981 tagtgtatta tggcctgtaa tgggagcaaa tccaaatata gattataata ttgcagaaat
     5041 atgggctcct agtagagaag atgacccaag agaaactttt gaacgaagtt ccagtaaggt
     5101 tataatggac ggtatttaca atagacattg tcattatatc gattggaaat tagaacccaa
     5161 catggattta tttggagtaa actgttcagt agatataata aaggaatata aaccagattt
     5221 aatgctaata catttagcta cattggacca tacacgacac aattatggct tatttaatga
     5281 cgaagtagat aaagcattaa aaatgaatga taaatggtta ggagatatta ttcaggctac
     5341 aaaggatgca ggtacataca aagatacaaa ttttataata cttggggacc atggtcatct
     5401 tagagttgat aaagttataa atccaaatgt cttgcttaaa tcaaatggat ttataaaagt
     5461 tgaaaatgga gaagttaagg attttgatgc ctatgtaaat tcttctggaa tatcagctca
     5521 aataatagta aataatttag ataggttaac agaattaaga gaactgcttg aagaatttaa
     5581 agaagaatta ttcatagaga atatttttac caaagaggaa gcatctaacc ttggtcttga
     5641 aggtgatttt gaaatggtaa ttgaaggtct tgaaggaatt tcatttggaa atgatttcga
     5701 aggaagtatt ataaaagatt cagatataaa agattacaag tttgctgttg cgacacatgg
     5761 tcatttacct actaaaggta atagacctcc atttatagct tttggaccaa atattaaagg
     5821 tggtgtggtt atagaagaag gagacctgag agcacatgca gcaactatat taaaaatgtt
     5881 taatttaaaa ttagatggag ttgaaaaaga agcatttgat tttattatgt aagttgaata
     5941 aagtatttat tttccatgaa ttatagactt ctattgcaaa gatagaagtc tttttttatg
     6001 ttttagattt aagtaaaatt taaatgggta aaatatttaa aataacttaa acaattttat
     6061 aaaggtatga tataataata catgtcagga actaatgttc gaaaaaagga gtgatactat
     6121 ggattttaaa ataaaatcag acttcaaacc tacaggtgac caaccagaag ctattaaatc
     6181 aatagtggat tctataaata gaaatgagaa attttcgaca ttattaggtg taacgggttc
     6241 aggaaaaaca ttcacaatgg caaatatcat tcaacaagtt aagaaaccta ccctaataat
     6301 ggctcataat aaaactctag cagcacaact ttacagtgag tttaaagaat ttttcccaga
     6361 caatgcagtt gaatactttg taagttacta tgattattat caaccagaag catatgtagc
     6421 tcacagtgac acttatatag aaaaagatgc aagcataaat gatgaaatag ataaacttcg
     6481 acattctgct acagcatcta ttttagagag aagagatact ataataatat catctgtatc
     6541 ttgtatatat ggacttggtg acccaaaaga ctataaagaa cttatgttat ctataagacc
     6601 aggtatgcaa agagacagag atgatgtaat aaaaaggtta atagagatac aatacgaaag
     6661 aaatgacata aactttacac gaggtacctt tagagttaga ggagatatac tagaaatatt
     6721 cccagctagt aatgatgaaa aagcaataag aatagaattt tttggtgatg aggtagatag
     6781 aataacagaa attgattatg taacagggaa gattgttggt actagaaatc atgttgttat
     6841 attcccagca tctcactatg taacaacgcc agaaagaatt gaaaaggcaa ttgtagaaat
     6901 agaaaacgag cttcaagaac aaattaaatt tttcaaggaa aatgataggc tattagaagc
     6961 acaaagaatt gaacaaagaa ctaagtatga tatagaaatg ttaaaggaaa taggattttg
     7021 tcaggggata gagaactatt ctagacatat tacaggaagg tcagaaggag aaagaccata
     7081 tactttaatg gatttcttcc cagatgatta tttgataatt gtagatgagg cacatgtcac
     7141 tataccacaa gtaagaggta tgtatgcagg agacaggtct agaaaaacat cacttataga
     7201 gaatggtttt agattacctt ctgcacttga caatagacca ttaaattttc aagaatttga
     7261 aggaaatata aatcaaatgt tgtttgtaag tgctacacca gggccttatg aaattcaaca
     7321 ctcagagaca atagcagaac agataataag accaactggc cttttagacc caattgtaga
     7381 agtaagacct ataaataatc aaatagatga tttggtagga gaaataacta aaactataga
     7441 aaaaaatgaa agagtgctta taaccacttt aactaaaaaa atgagtgagg acttgactaa
     7501 ctatcttaaa gaaataggga taaaagttaa atatttacac tcagatatag ttacattaga
     7561 aagaacagaa ataataagag atttaagatt agggaagttt gatgtccttg taggaataaa
     7621 cttacttaga gaaggattgg atataccaga agtttcactt atagcaattt tagatgcaga
     7681 caaagaaggt ttcttacgtt ccgaaacagc tttaatacag actattggac gtgcagctag
     7741 aaatgaaaat ggaagagtaa ttatgtatgc agacaggata actgactcaa tgcaaaatgc
     7801 tattgatgaa acaaagagaa gaagagatat acaaaattta tataatgaag agcacaatat
     7861 cattcctaaa actatacaga aaaatataag agatagtata gaagctacta aggttgcaga
     7921 ggaagaagtt gtatatggaa tatctgatac agatgataaa gacgaaataa gagctaatat
     7981 agataaatta aaatcagaga tgatggaagc tgctcaaaac ttacaatttg aaagagcagc
     8041 agaacttaga gataaagtga aacaattaga agaaaaattg gaaaaataga aaggtaaaaa
     8101 caacattatg gaagataaaa ttataataag aggagcaaaa gaacataatt taaaaaatat
     8161 agatttagaa ttaccaagag ataaatttat tgtttttaca ggtttgtcag ggtctgggaa
     8221 gtcttctttg gcatttgata caatatatgc agaaggacag agaagatatg tagaaagtct
     8281 atctgcttat gcaagacaat ttttaggtca gatggaaaag ccaaatgtag aatatataga
     8341 gggtttatca ccagctatct caattgacca gaagactacc tcaaaaaacc cacgttctac
     8401 ggttggaaca gtaacagaaa tatatgatta tttgagacta ttatttgcaa gagtaggaga
     8461 tgtgtattgc cctacatgtg gtgaaaaaat aagtcagatg actattcaag aaatagttga
     8521 taaaatgcta gattttccag atagaacgaa attacaaatt ctttcaccaa ttgttagagg
     8581 acaaaaaggt acacataaaa aagtctttga taatatcaaa aaagaaggat ttgtaagagt
     8641 tagagttaat ggtgaaaact atgaagttag tgatgatata atcttaaata aaaatcaaaa
     8701 acataatata gaagttgtag tggacagaat agtaattaaa gatggaatag aatcaagact
     8761 agcagattca attgaaactg cagttaagct atctgatggt cttgtaatag cccaaataat
     8821 agatggagaa gaaattcttt tttcaactaa atttgcatgc ccagaacatg gaattggcat
     8881 agaagaatta tctccaagaa tgttttcatt taatgctccc tttggtgctt gtgatgtttg
     8941 taatggtctt ggggaaagta aagaagttga cccagaatta gtagtaccaa acaaagattt
     9001 atcaataaaa caaggtgcag tggcagcatg gggttcaaca ggtgttaatg atgacactta
     9061 ttacagtaaa atgataaaaa gtttagcaga gcattttaat gtatctttga caacaccgtt
     9121 taaagattta ccagatgatt ttgtggagga attactttat ggtaaagata atataattgt
     9181 agagtttact tatgagtcaa aatttggagg aactagaaat tataaatcat actttgaagg
     9241 tgtaatagta aacttagaga gaagatatag agaaactaat tcagattaca tgagagataa
     9301 gattgaagaa tacatggcag agagaccatg tccaaagtgt aaagggatga gacttaaaaa
     9361 ggaagtttta tctgttttag ttgatggaaa aaatataatg gaagttacaa acttatctgt
     9421 aaatgaattg attgacttta tggaaaatat aaatcttaca gaaaaacaga gatttatagc
     9481 tcatgagatt ataaaagaga taaaaggaag agctatgttc ttaagagatg taggtcttga
     9541 ttatttaaat ttatcaagaa aagctggaac tttatcagga ggagaagcgc aacgtataag
     9601 attagctact caaataggtt ctgcattggt tggagtttta tatgtacttg atgagccaag
     9661 tatagggctt caccaaagag ataatgatag gcttataact actcttagac atctaacaga
     9721 tttaggaaat accttaatcg ttgtagagca tgatgaagac acaatgagag aagctgatta
     9781 tgttatagac attggtccag gtgcaggtat acatggtggt gaaatagttg cacagggtac
     9841 tttagatgaa ataattgaaa atgagaactc aataacgggt caatatttaa gtggtaaaaa
     9901 acaaatattg cttcctgaga ctactagaga aggaaatgga agttttatag aaatagttaa
     9961 agcatcagaa aataatctga aaaatattga tgtaaagttc ccattaggta agtttacatg
    10021 cataacaggt gtttcaggtt ctggaaaaag tacattaata aacgatatac tttacaaagg
    10081 tgttgcaagt aaagtgaata gacttaagca aagagctggt aagcataagg agataaaagg
    10141 aattgaaagt atagataaag ttataaatat agaccaaagt cctattggaa gaacacctcg
    10201 ttcaaatcct gctacttata caggtgtatt tgattttata agagatattt ttgcatctac
    10261 taatgaagct aaagcaagag gttataaaaa aggtagattt agttttaatg taaaaggtgg
    10321 tagatgtgaa gcttgtaagg gtgatggaat aataaaaata gaaatgcatt tcttaccaga
    10381 tgtatatgtt ccttgtgaaa tttgtaaagg tgaaagatac aatagagaaa cattgcaagt
    10441 aaaatacaaa gataaaacta tatctgatat attagatatg aatgtggaag aagctgttga
    10501 gtttttcgaa aatataccaa atataaaaag aaaattagaa actttaatgg atgtaggact
    10561 ttcatatatt aaattaggac agccttcaac tcaattatca ggaggagaag cacaacgtat
    10621 aaaattagct gctgaactta gtaaaagacc aacaggaaag actttgtata tattagatga
    10681 acctactact ggacttcata tggcagatgt agacaagcta atccaagtat tacaaagact
    10741 agcagataca ggaaatacaa ttatagtcat agaacataat ttagatgtta taaagacttg
    10801 tgattatgta atagacttag gaccagaagg cggagataag ggtggaacta tagttgcaac
    10861 tggaacacca aaagaagtat ctaaagttga tggttcttat acaggtcaat tcttaaagaa
    10921 atatttttct taagttatat aaattgtgaa aaattttaga gcagatatac gataaactgt
    10981 tttgttataa gtaaattagt caaatataag ttgtaaatag taatacaaag taaagtccat
    11041 attactcttc atggttaaaa ttgttttcaa atcaattata gaagggaata tgggctttaa
    11101 cttgtttaaa gcactggaaa ttgtataact ttttaaaatt catattttag ggtataattt
    11161 tagtataaaa tttaatgttt tggagggcga gatgtttgat atacaagagc atttaaaaaa
    11221 attaccttct gagcccggag tatatttaat gaaagacaaa tacgaccata taatatatgt
    11281 gggaaaagcc atatctctaa aaaatagggt aaggcagtat tttcaatctt caaaaaacca
    11341 tacatctaaa gtaaaatcta tggttaagaa tatatataag tttgagtata ttataacgga
    11401 ttcagagtta gaggcattaa tacttgaatg taatttaata aaaagataca gaccaaagta
    11461 taatgtagtg cttagagatg ataaaactta tccatatata aaagtaacta cgaatgaaga
    11521 ttatccaaga atcttaaaag ttagaagagt tcttaaagat aaggcaaagt attttgggcc
    11581 ctataccaat ataactgctg tgaatgatac cttggagctt ataagtagta cgtatccaat
    11641 aagaagctgt aaaatagata tagataaagc aataaagaat aagacaagac cttgtttaaa
    11701 tctacacata aataaatgtt taggaccttg tacaggaaat gtttctaaag aagagtatgg
    11761 aaaaatgata gaagagatta taatgtgttt atctggtaaa gaagaaaaat tgatggaact
    11821 tttaaaggaa aaaatgaatg agagttctat gaactttaga tttgaagagg ctgctgtata
    11881 tagggataag ataaagagcc ttgaagagat gatacagaaa caaaaaatag atgctactgt
    11941 aagtgactta aaccaagatg tggttgcaat ggcaagagct cataatgaag cttgtgtcca
    12001 ggtatttttt ataagaaatg gtaagattgt aggtagggaa cattttatat tagaaggggt
    12061 aatggatagt ccaagagctt ctatattaag ttcatttgta aaacaatttt ataatgaaca
    12121 agagtatata cctaaagaat taattataga agatgagata gaagatagtt ctatattaga
    12181 agaatggctg tcatctaaaa aggggcaaaa agtaacaata agagttcctc aaaagggaga
    12241 gaagaaaagc ttagttgaaa tggttaggaa aaatgctgta gaatatcttg aaaaattttc
    12301 tgatatgaat aaaagaaaat atgaaaaaag tattggagca ttggaagaat taaagcaaat
    12361 attaaatcta gaaaagttgc caataagaat agaggcttat gatatatcaa atatacaagg
    12421 tgtagattcg attggttcta tggtagtgta tacgaatgct aaaaaagata agaaggaata
    12481 taggagatat aaaataaaga cagttattgg acctaatgat tatgattcta tggctgaaat
    12541 tgtagataga agattaaaac atggaaattt acctgacttg atattgttgg atggtggaaa
    12601 aggacaagtt agtgcagtaa aaaaagtact agaactcaat gatgtagata tacctctttg
    12661 gggaatgtac aaagatgata agcatagaac taaaggattg atatgcaaag aaaaagaaat
    12721 agaactagat aaaacaacta atttatatag atttattgct agtatacaag aagaagttca
    12781 taattatgca ataacatatc atagaagtct aagaaataaa gctttgacaa aatctatctt
    12841 agatgatata caaggaatag gtgaaaaaag aaagaaatca ttattaaatc actttaaaga
    12901 tgtagatgct ataaaaaaag ctactatgga agaactttta gaagtggatg gaatgaataa
    12961 aagcattgca gataatgttt ataatttttt tagaaaggaa gaaaattaat ggaaactagc
    13021 aataaagtat caataagaga gataataaaa gagttgaagt taaaagtagt atacatgcct
    13081 gatgatgtag attattatgt ctactctcaa gatataaata gaccaggatt acagtttgca
    13141 ggatattttg attattttgc atatgaaaga atacaaatag ttggaaagac agaatataat
    13201 ttttttggat taatggatag tcatattcga gaagaagtac tagatagatt tttctcatat
    13261 gaaataccag cgttaataat ttctagagac ttggaaatca agccagatgt aattgaaaag
    13321 gctaaaaaat ataaaagaat acttttgagc acaaatagaa ataccactag acttataaat
    13381 agactgtcaa actatttgga taataagtta gcaccacata caacaattca cggggtgcta
    13441 gtagatattt atggtatagg tgtactgata aaaggtgaaa gtagtatagg aaagtcggaa
    13501 acagcattag aattgattca aaggggtaat agattagttg cagatgatgc tgtagaaata
    13561 agaaaattag atgaaagtat gcttgtgggt cagtcacctg aactaataag acattttctt
    13621 gagataagag gaataggaat aatagatgtt agaagtttat atggagtagg ggcaatcaaa
    13681 aattctaaaa tgatagattt agtagttcat ttagaagctt gggatgaaac taagtattat
    13741 gatagactag gactggataa agaatatgaa gaaatacttg gtgttaatgt agagaaatta
    13801 gttgtacctg taaaacctgg aagaaatact gctatgatat tagaagtagc agcaatgaat
    13861 tttagacaaa gaggaatggg atacgatgca gctcaagaat ttactaaaaa attatcaaaa
    13921 ttaatcgata ctaagtagta ctgaataata gtgaactaca catcatttaa ttaaatattg
    13981 attaggatta tgttctacac cttataaatt aaaatcaagt tgtatatata taatgtttat
    14041 taataagata tattatataa ggataattta aagtacagtg ttagattata ataatactag
    14101 attaatttta tatttgagtt tcattatata acaagaaagt tggaattttt tatgggaata
    14161 gaatatttta gtaaactttt agaaagtagt actgaaaaag aattgctctt aagtaaaaag
    14221 tcaaataaca taagtacagc aagattaata tcttttttga tagtgatagc agggcttgct
    14281 attggatttt acaataaaaa tatagtaggt atttttgttg gcgttttatc aataataata
    14341 tttatagcac tcttagttat tcatagtaaa gtaaaagaag aagaaacata ttttaaaagt
    14401 aagagcgaag tattaaataa gtatattaag agatttggag atggttggaa ggaatttaaa
    14461 atagatggta aagaatactt aaaagatgaa aattcgcaag caaaagattt agacttattt
    14521 ggaagagcat cattgtatca atatatctgt gtagctaata catcttatgg gaaaaatttt
    14581 ttagccaaat atttgtggaa tgaaaatcca gatgagaatg taatcttaga aagacagaga
    14641 gctataaaag aacttttatc aaaagatgat ttttctattc atattcaaac actcagtaat
    14701 ataattggta aagagcaaaa agataattca gatagtagta tagaatcgtt tatagagtat
    14761 ggagaaaata agaatgtgta tataccaaaa tggatgcata tttttacttg gggtttacca
    14821 actgcaacaa tcttatcttt tatattttgt atgttaggat ttttacctgt tttacctgtt
    14881 tttttacttt ttataatcca gttagggttt agtggttttg gatatccaag acttatgcaa
    14941 actcttgctc cattattttc atttagtaga agtatacaag tatatgaaaa aatgtttgag
    15001 gttttagaaa aagagacgtt tgagagtgca tatttgaaag aattacaaga taaactttct
    15061 aagggaagtg gagtttcaag aggtgttaaa caattaaatt caataggaaa tgctgtaaac
    15121 ctaaggtata atcagatact atatataatt gcctgtggtg ttttgatgtg gaactatcat
    15181 tgtgcagaag ctttggagcg atggaaaggt atatatggaa atcaaattag agattggttt
    15241 gaatctattg gagaatttga agctctaata agtttaacag ttgtttctca tgtaaaggaa
    15301 aatacatgtt ttccagtaat aaaatatgaa gatactccaa gattgaaagt tgaagaggta
    15361 taccacccat taatagcaga aaaaagtgtc atagcaaatt cgatacaatt aaattctcag
    15421 acttgtatta tcacaggttc aaatatgtct ggaaagacaa catttttaag aagtatcggt
    15481 gtaaacttag tgctggcata tgcaggtgct ccagtatgcg ctaagagttt tgatgcgacc
    15541 tgtatggcta tatttacttc tatgagaata caagatgatg taagtcaagg aatatcaact
    15601 ttctatgctg agattcttag aataaaatca atgattcaat atagtttaaa agaactgcca
    15661 atgttggttc tagtagatga aatttttaag ggaactaact ctgctgatag aattattgga
    15721 gcaagtgagg cagtaaagaa attatcaaaa ccctgggtta taagtatggt gacaacacat
    15781 gactttgagc tatgtgacct ttcaggtagt ggagatattg aagttgtaaa ctatcatttc
    15841 tcagagtact atgtagacga taaaattcat tttgactata agataaaaga tggaagatgt
    15901 aaaactacaa atgcaaaaca acttatgaga atggcaggta ttctttaatt aatagatagg
    15961 ttaattaaag aataagtcta tttagaaata taatttaatt tgtgaaatta ataaggattg
    16021 tcttaaatta cgtaaaagta ttttaagaca atcctttttt aagaataaat tttatacttt
    16081 gttatgacta cttagcttct tcacaagcac attcttgtgg acactctcca tagacacaac
    16141 tcttatcaaa gtaatgagta tgaaggtatt tatgagctac atcactattt ggttctccaa
    16201 agaagtcttt ataaatagct tgtaaatcag gattctcatg agatttacga atagccttat
    16261 ttctatctat ttcatataac gcatttgcac gtctatcaac tatttcagta ttaccatggt
    16321 gataaggttg accagcacct ccaacacatc caccaggaca tgccataact tctatgatat
    16381 ggtatttaca ttctccactt ctgaccttat ccataatctt tctagcgttt ttaagtgtat
    16441 ttacaataca cacattaact gtagttccat caacatctat acttgcttcc ctaattcctt
    16501 taagacctct aacattagtg aagtttacat tatctaaagt cttattagtt atcttctcat
    16561 atgaagtacg tagggcagct tctaaaactc caccacttgc tccaaatata gatgcagcac
    16621 ctgtagattt tcctagagga ttatcaaaat cttggtcttg aagattaggt aaatcgatag
    16681 cagcttcttt tatcatctta gctaactctc tagtagttat agaaaggtca acatctagta
    16741 ttccagattc acttaattct tctcttgaag cttcatattt tttagctaca caaggcatta
    16801 cagatacaac ataaagttct tcaggattga ttcctaatat tttaggtgca tagtagtttt
    16861 tagctataga accaaacata ccttgaggag acttacagct agatgctaag tttaacttgt
    16921 ctggataatt gtgctctaag aagtttaccc atgcaggaca acaacttgtt aatataggaa
    16981 gattttcacc tttttggatt ctttctataa attcagtagc ttcttccatt atagtaaaat
    17041 ctgcaccaaa gtttgtatca aatacatgtt caaatccaag tgcttttaaa gcagctacca
    17101 tttttcctgt agagattgac cctggttcta atccaaactc ttcaccaata gcaactctta
    17161 cagcaggagc aacttgcact actatagtct tttctttttt atttaaaaca tcccaaagtt
    17221 taggtacatt atctacttct gttaatgctc cagttggaca gacagatata cattgaccac
    17281 agaatgtaca ctctgtttca accatatcag agttaaagaa tgtactaact aaagtattaa
    17341 atccacgatt aactccagat aatgctccta ctgtttgtat gtcattacat acagtctcgc
    17401 atcttctaca taatatacac ttagaatggt ctttaactaa agatttagta gaagtatctt
    17461 tacctgcaaa agatttagaa ccttgatatc ttatttttct tactcctaaa tcagcagcta
    17521 tagtctgtaa ttcacaatct ccattttttt cacagacaaa acaatcttgt ggatggtctg
    17581 ataacaataa ttcaacaata gttctacgag catttaatgc tttagcacta ttagtctgaa
    17641 ctttcatacc ttcctttata actgttccac aagctggaac taatcctctc tctgtctcaa
    17701 ccatacaaac acgacaagaa gcacaagtgt caagcttatc tatttcattc atatgtaaat
    17761 gacaaaggtt gtgtattttt atatttatta atttcgcagc atctaagata gaagttccac
    17821 taggtgctga aacgtgtttg ccattaatag ttaaattaac taaactcatt gttattcctc
    17881 ctccaaataa gttatttctt gattatagcg ttaaatgtac aattatctat acaagcacca
    17941 cattttagac acttagaagt atctattgta tgttgttcct taacagaacc tgttatacaa
    18001 ccagcaggac acactctaga acattttgta caacccttac agtcatctgt tataaagtaa
    18061 gaaagtaagt tctgacattt accagcagga catttcttat ctacaacatg agctatatac
    18121 tcatctttaa aatattgaag agtacttaaa acagggtttg gagcagcttt accaagacca
    18181 cataatgaag cagtttgtat agtttcagca aggttttcta ggtcatctaa atcttgtaga
    18241 gtaccattac cttctgtaat tttagttaat atttctaata atcttgtagt acctatacga
    18301 caaggtgtac atttaccaca agattcttca acagtaaact caaggaagaa cttggctata
    18361 tctaccatac agtcagattc atcaagaaca agcataccac cagagcccat cattgaaccg
    18421 atagaactta atgaaccaaa gtctatagga gtgtctaaat gttcagttgg tatacatcca
    18481 ccagatggtc cacctgtttg aacagcctta aatttcttac cttcagggat tcccccacca
    18541 atttcaaata caatatctct taaagtagta cccattggaa cttcaacaag tcctacattt
    18601 tctacctttc caccaagagc aaagacttta gtaccactag aatcttcagt acctaagttg
    18661 gcaaaccaat ctccaccctt aagtataatt gcaggtatgt tagcaaaagt ctcaacgtta
    18721 tttaagcaag taggagattt ccatagacca ctcttagaag aactataagt tttcattcta
    18781 ggttcaccac gtctaccttc aattgaatgc attagcgctg taccttcacc acaaacgaaa
    18841 gcacctgcac catattttaa ttctaattta aagttaaatc cagtgcctaa gatattttcg
    18901 ccaagtaaac cgtatttttc agcttgagct atggctactt ttaatctttc tatagatttt
    18961 ggatattctg ctcttatgta tatgtatcct gtatcagaac ctatagcgta tccacatatt
    19021 gccatagctt ctagtacact atgagggtca ccttcaagta cagatctatc cataaatgca
    19081 cctgggtcac cttcatcagc attacataca acaaactttt tatctgttgg accagctgga
    19141 ttttttgaag cagcttccca tttagagcca gtagggaaac ctgcaccacc tctaccacga
    19201 aggccagaag tttttacctc tgcaataact tcttgaggag taagagaagt taaacatttg
    19261 cctagagcta aataaccatc attggcaata tattcttcta tactatctgg gtctataaga
    19321 ccacaatttt ttaaagctat acgtaactgt tttttgtaga aagacatttc ttcttgttct
    19381 tcaacttttt tacttaaaga ttcttcctca tataataatt cctcgacaac tgtatttcta
    19441 attaagtggc tttgaactat cttttcagca tcagatggct caacttttac gtagaaaacg
    19501 ttatcaggat aaacttttac aataggacct tgagcacaga aaccaaaaca acctgtcaaa
    19561 cgaacactaa ctttatcttg gattcctgat ttctttattt cattttctaa ttcactaact
    19621 atttctagac tgtttgaaga tgtacatcca gtatcacaac aaacaagtaa ttctcttctt
    19681 aatacatttt ccttagaata attttttctt aagctaagat taggtttcag ctcatcagct
    19741 aaaactttta attcatcaaa tgaatttact ttacgcatta tttattcacc cctcttaaca
    19801 attcaattct aattctctat aagtatctaa tatttcgctt acttggtcag gtttcacttt
    19861 accataaact ttaccattta cagtaacaac tggagctaaa ccacatgccc ccaaacatct
    19921 taaaccttct aaagaaaatt taaaatcact agtagtttct ccaactttta tgccaagctg
    19981 tttttcaaaa gcacttaaga ttttgtcagc accttttaca aaacatacag ttcctagaca
    20041 gacactgatt ttatacttac ctacaggttc tgtagtaaaa taagaataga atgtaacaac
    20101 cccatatacc tttgcaggag ctactccaag ttttctagca acatgaagtt gaacttcttt
    20161 tggaagatat ccaaatatac cttgggcttc atgtagaact tgtattaacg caccttcctt
    20221 tgttgcaaga gagtcaataa atatatctag ttcgtcaaat aattgcttgt tgtgtgaaat
    20281 aaaatcgcac ataacaaatc ccccttttct ttttaaagtt attatttttc ataaaataat
    20341 taaccatgag ttgaccccaa cattatgtgg gtgaacaaaa ccaaaacaat aaagaaagaa
    20401 taatatacaa caatttcttt atttataaaa acaatgcaaa aaagttacct attaaaaata
    20461 aaaactatac tagtctatta taatataaaa taattcagaa tagttaaaaa aaataaaaaa
    20521 aatgagctaa attcaataaa aaaaaaataa tacatataaa aaactttaaa aaataaacca
    20581 aaatgtaaaa tttttataaa ataaaagatt tataaaactg ctgataatag tcttgaaatt
    20641 gattccttga tttttatata agtagaccta gaattatata tatctaatgt tatttcctta
    20701 gaatctaaaa tatcattttc aaatataatt ctttgttcca atgcttttct tgaagagtac
    20761 ataaatgcat taacttcgaa attaagctca aaacttctga tatccatatt agctgttcct
    20821 atagagcata tagagtcatc tataactatt gtcttagcat gtaaaaatgc attttgacca
    20881 taagtgtaga ttttagctcc aaactttaaa agttctccag catatgaata tgatgcccag
    20941 tatacaaagg gatggtcagg tttagaagga atcatcactc tcacatcaac accagataaa
    21001 caagctattt ttaaagtatc tataaaagtt gagtcaagta ttaaataagg actctgtatg
    21061 tatatatact ttctagcttt ctgaatcatt ttaaggtaac catatttaat ctcatcaagt
    21121 tctgatatat ctggaccact tgatactatc tgaatcccaa tattttcaga aggatgatta
    21181 gaatttgtat cttcttctat aaagtacttt tctaaatcta aatcttcttt agttgtatat
    21241 ctccaatcaa ggatgaatct catatttagg tcaattacgc aatcaccagt aagcttagta
    21301 tgtgtatctc tccatagacc aaattttgga tttctaccta aatattcatc accaacatta
    21361 tatccaccta caaatcctac cttaccatca atgacaacaa tttttcggtg atttctatag
    21421 tttaagttaa agtttacaat tttcaagaat gatgggaaga agcttccaac tttaacacca
    21481 ctttctttaa gtctggaaag tgtactattt tttagtgttc taccaccaac tgcatcaaat
    21541 aaaagtctta cttcaacacc ttctttagct ttgtctataa gaatgtctat tatttcagtt
    21601 ccgattttgt catctttgaa aatgtaaaat tgtatgttta tatatttttt agctttttta
    21661 agttcttcaa gaagactgtt aaaaaattga ctagattcag cataaatcca cacatcgtta
    21721 ttattggtat aatgagcatt atttgaatta gccaatgcat aaatcatatc cttatgttga
    21781 tgtatatctg aatcaatttc agatgtagat tggagtttaa cctgagtatc tagtatatta
    21841 cttttgatta tcttatcatc cttttctttc aatctaaaca tattgttttt tgaaatattt
    21901 ctgccaaaag ctatataaag tatgaatcca agagcaggaa tcaatactaa tataagaagc
    21961 catgttaaag ttgtatttat gtttcttttt tctctaaaga taaggtttag tataaccatg
    22021 aaattaatta tatatattgt agtaactgct at
//
LOCUS       DAEMIK010000064        21911 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEMIK010000064 DAEMIK010000000
VERSION     DAEMIK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.064"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..881
                     /locus_tag="KPK87_002005"
     CDS             <1..881
                     /locus_tag="KPK87_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2538155.1"
                     /translation="LIMNKYKFYKELDILKRGILTFFISCFALILFMEGTKYIQLRLG
                     YSFLLEGVLNIILFFVLIILSFYTGFKVIRLINNNNEQEYKLQKLDSKINHKDNIIIK
                     LRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFKSKNSRYGKVSYLDAIASLK
                     YEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDNAIESLSTIKKEKKYIRLHA
                     YEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGYGLHLVKNILDKYDYSILVN
                     SDDFLTEFVILIPKYY"
     gene            900..1637
                     /locus_tag="KPK87_002006"
     CDS             900..1637
                     /locus_tag="KPK87_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2538156.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKCLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(1822..6879)
                     /locus_tag="KPK87_002007"
     CDS             complement(1822..6879)
                     /locus_tag="KPK87_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG2538157.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNTQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDHSILSNL"
     gene            complement(6904..7323)
                     /locus_tag="KPK87_002008"
     CDS             complement(6904..7323)
                     /locus_tag="KPK87_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538158.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(7556..7807)
                     /locus_tag="KPK87_002009"
     CDS             complement(7556..7807)
                     /locus_tag="KPK87_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538159.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            7896..8627
                     /locus_tag="KPK87_002010"
     CDS             7896..8627
                     /locus_tag="KPK87_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG2538160.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            8686..9597
                     /locus_tag="KPK87_002011"
     CDS             8686..9597
                     /locus_tag="KPK87_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2538161.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            9648..10460
                     /locus_tag="KPK87_002012"
     CDS             9648..10460
                     /locus_tag="KPK87_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG2538162.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            10569..11261
                     /locus_tag="KPK87_002013"
     CDS             10569..11261
                     /locus_tag="KPK87_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2538163.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRFRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            11348..12289
                     /locus_tag="KPK87_002014"
     CDS             11348..12289
                     /locus_tag="KPK87_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538164.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYKNMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            12326..13483
                     /locus_tag="KPK87_002015"
     CDS             12326..13483
                     /locus_tag="KPK87_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2538165.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            13635..14195
                     /locus_tag="KPK87_002016"
     CDS             13635..14195
                     /locus_tag="KPK87_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2538166.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            14223..14714
                     /locus_tag="KPK87_002017"
     CDS             14223..14714
                     /locus_tag="KPK87_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538167.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            14943..15494
                     /locus_tag="KPK87_002018"
     CDS             14943..15494
                     /locus_tag="KPK87_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538168.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            15580..16512
                     /locus_tag="KPK87_002019"
     CDS             15580..16512
                     /locus_tag="KPK87_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG2538169.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            16567..17430
                     /locus_tag="KPK87_002020"
     CDS             16567..17430
                     /locus_tag="KPK87_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2538170.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            17677..19539
                     /locus_tag="KPK87_002021"
     CDS             17677..19539
                     /locus_tag="KPK87_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2538171.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTKFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            19542..21008
                     /locus_tag="KPK87_002022"
     CDS             19542..21008
                     /locus_tag="KPK87_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2538172.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            21032..21868
                     /locus_tag="KPK87_002023"
     CDS             21032..21868
                     /locus_tag="KPK87_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2538173.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         8262 a         2473 c         3592 g         7584 t
ORIGIN      
        1 atcttataat gaataagtat aaattttaca aagaattaga catattaaaa agaggcatat
       61 taacattttt tatatcgtgt tttgcattga ttttatttat ggagggcact aaatatatac
      121 aattaaggtt aggatatagt tttttacttg aaggagtatt aaatattata cttttttttg
      181 ttttaattat tttaagtttt tatacaggct ttaaagttat aagattgata aataataata
      241 atgaacagga atataaacta caaaaactag atagtaaaat aaatcataag gataatataa
      301 ttatcaaatt aagaagtcaa aaacatgact atttgaaaca tttgcaagta gtgtattctc
      361 tattaaatac tgggctagat gaagatgcca aagaatatat aatgaatata tcaaatagct
      421 ttaaatcaaa aaattcaagg tatggaaaag tatcttattt agatgctata gcttcattaa
      481 aatacgagga atgtcttgaa aaggatatat cttttgatgt ctacattcaa gattttatgg
      541 ataatttaga gattgaacct aatgatatga gttcagtact attaaacata atagataatg
      601 caatagagtc tttgtctact ataaaaaaag aaaagaagta tataagatta catgcatatg
      661 aagatgaatt tcagtatatt atagctataa aaaataatgg tcctaaaata cataatgttg
      721 aaagtatatt ttggaatgga tttagtacta aagaaggaca agatagggga tatgggctac
      781 atttagttaa aaatatatta gataagtatg attatagtat acttgtaaat agtgatgatt
      841 ttttaactga atttgtaatc ttaatcccaa aatattatta gttaggagga gaaaagtcta
      901 tgataaaagt tttgatttgt gatgatgata agattgtaag agatgatata gcaaatatag
      961 ctaaagagtc tggatatgta gattatgtta aaaaagttaa ggatggtata gaggcaattg
     1021 aatttataaa aaatgagaaa atagatttat tgattattga cattgatatg ccatataaaa
     1081 atgggattga ttctgctgaa gaaattcttt ctatagataa ggatatacat atagtatttg
     1141 tgacgggttt cgctgattat agtcttgaaa gctttaaagt gcatcctata gattttgtag
     1201 ttaaaccatt taaaaaagaa aaaatacttg attcaattaa catagcaata gaccatatta
     1261 actcccataa gattgctaat aataatttta tagaagacac tctttttgta tataaaataa
     1321 ggaagcagat acatatgatt aattttgatg atatagttat gtttgagaaa aattcaagag
     1381 caattaatct acacactaaa gatgaagata taataaagtt ttatgagaac tttgatgatt
     1441 taaaaaaatg cctacctcct aatttcttta tgacccatag acaatacata gtaaatctaa
     1501 gatttataca taaggttata cctataaata aatcttcatt agagataagg tttataaatt
     1561 ttcaagaaaa tgccatatta aacaaatctc tagaaaaaga ttttttatat agattttata
     1621 gggttaaaag attctagtga agatagaaga aaaatacttt ttgtgtgata taataagtac
     1681 cgtaaagata atgtataaga gaagtttata taaaaattat ttgtaattat gatttaatct
     1741 atataaatta cagtatagta tatattggac tttctaattt ttttaagaaa gtccaatata
     1801 ttttagggat agtttttata cttataaatt acttaagata ctatggtctt taatcttact
     1861 atcttcagca agtaatagta tcttagacat aatctcagca gttttagggt cttcatcagc
     1921 gaatggaagg aatatcttac ctctactttg agaatgtata ggaagtatat tcaaagcacc
     1981 catccctttt ttatgtacaa taccagaacc catatgcaca gaatactcac ctaaattacc
     2041 tgctataaga gcatgagagc cttcaaaatt gacattgtta atttttaata ataataaaag
     2101 ttctttagca attgctatac gcatctctac agtagaatgg ctagtttcag ggtcaacacc
     2161 accagcatga gcgacactta caacaaggtc tatatcacgc ataacttctg agaatagaat
     2221 tggaggtata gaagctaatt ctaaactttt ataagttttt ctatcaaaaa attctactgt
     2281 ttcaatagta ggaggctcta tatctgctgg tgaaaaccaa tctgcaagtg caaacatctt
     2341 tgccactata ttttctttat aaaatacttt ctgtaatccc tcttcataat cgacagtcca
     2401 aagctgattt ctaagaagtg ctacagtttt ctttggctga atttggtgac cagcatatct
     2461 gcgagatata gttttttcag ataattcatc actgtttatt ctataatact cacggaatac
     2521 ttgcttaaat ggttgaacaa tttgcttact aaataaatca tgttggaact cactccactt
     2581 accagattca aacaaatgaa ctggatgtgc aagttttaat ttattttttg ccgtcaactt
     2641 ataagtttca ccttctatat tgcaaataaa gaacttagac ttatcattat aaacatatcc
     2701 aatattatca tcacataccc atactagatt tttaatcaaa gggcagataa ctggattttt
     2761 atagatattg gcaatttctt taactgtaaa tactatttca tttttcatag cttgttctaa
     2821 actctgtttt gcacgagaat actgactctt taaatctttt tggacagatt taagttcaag
     2881 tacatatgga tgtttagcaa tagcttttgg tatactcttt aaagacttgt tgcctttttg
     2941 aactttagta gatgcttttc cgttttcatc aacatctatc caaattgaag taccatcaat
     3001 atcttttggt ttaaatagat gagcaacttc atttagtttt tcagtctcaa gagcccacat
     3061 catacgattt acatctccaa atccagcact tctagctaga ttttctagcg ctatagtaca
     3121 agcttttgat tcactttcac gacgttgtgc accaaattgc ttgctttctt tagcaaactt
     3181 ttgaataaat tcataacgga aagttgcttc ttttaggtct ccttttttca taggaagtaa
     3241 agagaaactt ctcaatttat cttgatttct atttgtttca atttctttca tcaacatgtc
     3301 tttatcaagc ttaccaagtg tagcatcagc atagagttgt gcacgtctat gagagttacc
     3361 accactagtt atatatttag ctgacttgta aagtatattg aatctttctt ccccaatagt
     3421 tttatatgaa tctaaaaacc aatctacatc aaatgcacca tcaatgaaac tacgtggtga
     3481 aataggggag aatagagcaa cctctgattc tttttgtgca gagaagcctt catttatatg
     3541 tgcatggaag taccaaattg tacttttcag acccttccag tttaaatatt tttcaattat
     3601 gtttacccat tgaggggcat acatagcaga ttctatcaat cttaactcag taatgtcggt
     3661 atttttaagt aaacttttta atttttttga atcatcttct ttagtaggat agcaattttt
     3721 aagtagagaa cttaaaacat ctttttttga ataattgcta ccatatgaat atcctctctg
     3781 gaaagtttct ttaccaagag cgaccaaaat atttacaaaa taatttatac cttcaaagta
     3841 ttgtatatta ttagctagat gagacacctc tgttggcata tcacctctac gtgattcaat
     3901 ttctataatt cggtttgtaa cagtttttaa attatcttta agccaagggt acttttctaa
     3961 tgtgtcgcca gcccatctag gatttgttaa tgttcttatt atatttgatg cgttgtatcc
     4021 acttatcatt tctctattaa cttgctcttc agtcattaat cctaattcat gtgcacgagc
     4081 aaacacattt atgctagtat agaaaggaaa cttatattct agcaactgat aatttgaaaa
     4141 cgtagtgtag aaaaacttag taaaagaaat atcatcataa ataaacgaat gtgctttatc
     4201 aagccaatag tttaaaatag gggcatttaa agcagggcag tattcatttt gtctgtaata
     4261 gtttttagta aattcaactc tttgtttttt acattgttct atagtcaata agtttctaag
     4321 tttatagtat atttctaaac aatagttaaa tatttcttta tggtcaaact catttagtaa
     4381 aactcttaaa agttttcttg taagtccatt aaatttaagt ttttcaagtt ttgtatatat
     4441 tttattttgt tttttagagt caaggacatt tgaatatata gatgaaaacc aacttttttc
     4501 tgtttcatta tatccacatg tacttatact ataaaataag atatcaagta ttaatttgta
     4561 atctaagtca agttttttac tagtttctct ccaaagctct gctaatggat aattatcaag
     4621 agtatcatta gaaactttat atttaatagg agtcaagtta taagtagaat aacctaaaag
     4681 tactttgtct acattacctg accatctttc aacttcatat tcataatctg cattttcagt
     4741 aataagctca tcaaattttt cataaacttt tattatttta tttatatccg tatcgagaat
     4801 tttttttgct tctttttttg ttgctacaga aacagaatcc tcacgagtag ataaacttat
     4861 atttgagata attttattaa gctttgatga gctattagaa tcatctttaa aatcaaattg
     4921 ttctggaatt tgaactttgt tttcagggtt atatattcca aaaccatttt ctttgcagta
     4981 atcatttttt gctttaccta acaaattatc aattagtatt tgaacttgag atgtattctt
     5041 tttagattta tcaatatctt ctataagtaa aacaagctta ttatatagtt cttgtctagc
     5101 agtattgtct tttaataagt ttacaatttc aagacctcca aggcattttt gagaatttga
     5161 agattttatc agtgaagaag caatattttc caagtttgaa tcatcttgtt caagtaaaat
     5221 ttttattact gtttgtctaa tagagcttgt ctttaaagac aatagattgg ttatcttatc
     5281 taattctaca tctgttagct caagctttct aagtagactc aataccgttt ctctattgga
     5341 aacactctta tcacttaaac attccaataa aaattcacgt tgtctatctg tctttggttc
     5401 atttacaaaa ttgactataa gtgaagctct taaatctgaa ctcataagat gacaattttc
     5461 cataagcaca tctataaatt gagtattcat atcgtatgct gttaaagtca tcatacattt
     5521 aagtatttca tgagaagaaa tttcaatcca agtccatgga aatacacttc ctgaaaatga
     5581 ttttgtcttt gaatttaaaa tttctaaagt atgttttaaa ctttcaaatt gttcttttct
     5641 agcatcaaag tctttgtcaa taggagtgtt tgacaagtca aaattagttt ctctgttttg
     5701 ccaactatag tagtagccaa cagaaccttc tacatagaaa tttgtaatta cccatgctag
     5761 tatctctaaa tcatcaccct taatgttttt agatgctata gaatgtttaa aatatgtact
     5821 ttctgtatgc gataaaaagt aaagagctac aagtttttta tatttttcct gacaagaaag
     5881 taagttttca ataagagagt aagagtcata tatgttgtaa gttgcagtag cccataaggc
     5941 aaaataaatt tcaagattat cattgctatt aattgattct atacggaact tttcatcagt
     6001 caatgattta tatgaaagtt ctaaacattt acgtattatt ccaggttttt ggtctgaaaa
     6061 accaagtcct gtccatgtat ccaaggctct tataacagaa ctaaatcgct ctaaattgtt
     6121 gtccaaaata gttttcaaca tgtatattga tgcagaaatt gtaccctcat ctaaactctc
     6181 tacaatttgc tgtctaagtc cttcttgttg tttggctgcc aaaaggagtt tagaaagcat
     6241 ttcatagcat ttatcactat gactcttcat gataccaatt atcatttgtc ttgtaagtag
     6301 agcagtttga ttttctccaa atattacttc ttctaaagca cttattactt tttgatttcc
     6361 tttgtctatt tctagtgcta ttttatcact cattatagag tcatagtaca agaccttatt
     6421 tggaagagta agataatcta taactgaaaa atcataataa attaaatcaa catagtttga
     6481 aataaacctt ataatacctc taaaataatc cacaagttta tttgagcgat accctttacg
     6541 ataatatcct cctgaatatg tataatccat ctttctttta attaattggc aaagttcttc
     6601 tacttgttca gaagtaaata ttaattctaa tgcatttttt aaaggtccag aaaggagctc
     6661 gtccatattt ttaatttcgt agtagtggca attgtagtat ttctttatca tatttaagaa
     6721 tttgtcttca ggttctctat aatagtatga actattttct atgcagtccc aaagagactt
     6781 tgctaagtcg tagtactgtt ttgaagaaga tttagtaaaa ttatttttaa aattttcaat
     6841 tattttagga tttttttcta ctctagtacg ttctaacatt tatgtacctc caaattattt
     6901 attttaaaac attcttccag caagcaaggt aagtcttata aaagtaaata tatctccact
     6961 tttcagattt atagaggaat cgagtttttc aataacatct tcatttataa gcactttgta
     7021 gattccatct tcaaatgcct gtaacgctac ctctattgcc ttttctacag attgtttatt
     7081 ctcattgtaa atagagccaa atccaacttt tccaatatca gcttgagatt caatttcttc
     7141 attagttaag aagtttatta ttgatttttc tgggattttc tcattatatt caagaacatt
     7201 tttagataca atttcagaga tagcttcttt taggcaattg atgttgtcag atacatcaaa
     7261 aggggtaagt tctagcatag gtttgcgttt gccaacagcc ttaactctaa aataaatttg
     7321 cataaaatca cctcgcaaaa tattaattat gtatttagaa atttaaaatt tatctatttg
     7381 ttatattata tcattttgtg aattattaat gtgaattatg gaattatttt acaaaaaata
     7441 gaaaaaaagc tatcttaaag tctaaaaaag caataaaaga taacttttaa ctataattat
     7501 atagaattat aactgtacat ttattaaatt ttaattttag aattattttt gataactatc
     7561 tttttttatt tataaatttt gctcctaaat attttttgat atcttcttca ggagaaaagt
     7621 catcaataag tatttctttc ccagtgtctt caaatactac aagaacttca cagtcacctt
     7681 gtaagaaaaa ttttctaact gtatcttctt taacttctct aacttttatt acattctttt
     7741 tttctttttc aaagtctttt gtgtaataac ttcccatcat attaattatt tttgtatatc
     7801 tattcataaa atacctccat atgcgattaa atttaaaaaa tatgttataa taattgtttg
     7861 tattaactta ataattataa caaggagtaa taataatgac tagtataaaa aataaagata
     7921 ttttaaattg tatagattat tccattaaaa ataagctctt tgacaaacta aatgatgtat
     7981 ataagtcctt gccagtagga aattgttctg gatgtggaaa ttgttgtatg gagtcagtag
     8041 gtataaattt aattgagttt ttgaatatat tttgttatct tgaagataga gttgatttaa
     8101 aaaaaagctg tataagtaaa atattagatt attattttga ggagtataca agaaagaacc
     8161 catgtccatt taaagatact aataatagat gtttaatata tgaagttaga ccattaaatt
     8221 gtaggatgtt tggacattgg aaaaaagagg attataataa aaatttagat aatgttattg
     8281 agaaaaataa taactataga gagttaatga aaaagcaata tggatttgaa ataaatgatg
     8341 aagtagtaaa ttacaggata gattattgtg atagatttat tccaaataaa gattatttaa
     8401 gtaaatctaa gagattaagc ttttttgatg agcttatgat attagactca aatatttatt
     8461 caagtggaaa tatagatata gactttaggg atagaggtat agttgaatat tttatagagt
     8521 cattacttta tagaaattta gcatataatg tgaaaataag gatatctaaa gaatacaaaa
     8581 taagaaaaag aactatagat agaataaaaa ggattgttct tgtatagttt tatattagta
     8641 agtaataaat tgtatcataa atattgtcaa aatgaggtga agaaggtgaa tcttacaaaa
     8701 gtaaaaggaa ataccttttt tataaaaggt ggcacgaata caggaatata tgtatttgaa
     8761 gataacacag cacttatgat agacccaggg ttatgtggtt caagacctag gagagtaaat
     8821 aaagtattag atgaaaataa tatacaggtt agatatatta taaatactca tggtcatgat
     8881 gaccactatg gagcttgtaa tcaatttaaa gaatattata aagatgtgtg tataatgtca
     8941 tctgaatacg ataaattata tatagaaaat ccagaattgt tttcaaagta tattgttggg
     9001 ggaaagagca atgtatttat ggataatatc ttaaaaaata aaatgttgga tgatataaaa
     9061 atagataaat ctatagaagc tggaaaaatt gaattaaaca atgaaatgtt tgatataatt
     9121 gaatttcagg gtcatacacc tggtagtata gctgttttga cgaaggataa tgtgattttt
     9181 gttggcgatt tattgatagg agatgaaatt ttatcaaaat acgatttctt gtttctatat
     9241 gatatacaag aacaaataaa ttcattagaa aaattaaaaa atatagattt tgagtactta
     9301 gttttagggc atggcaagca agttatatcg aaagaagatt cttataaatt aatagaaaaa
     9361 catttgaatg ctttaaaaaa atatttatat caaataagag agttgcttgt agaaccgaaa
     9421 tctttagaaa ttttattaaa aaacattata aataacaata atttaagcaa taattataaa
     9481 gagtatcatt tttttaaatc ttcgttagta tctgtcataa gttacttagt tgatttagaa
     9541 gaaatagggt atatattgga aaatggagaa ttgctttact atacaaaaac aaaataaatt
     9601 atgataaaat aaagaaggtc ataaaaaaca aataaagggt gagcaatatg gaatacgaaa
     9661 aatggaatga gatactagca ccttatgaac atgcagtaga ggaattgaaa ataaaattta
     9721 aaaatataag aaaagaatat ttagcaaaag gtgaatattc tcccatagag tttgtcacag
     9781 gtagaacaaa aaaaatatct tccataatat ctaagttaaa aaggataaat gcaaaagata
     9841 tagaaactga aattgatgat attgctggta taagaataat gtgccagttt gttgaggata
     9901 tatatgctat agttgatttg ataaaagtaa gaaatgatat gactataata ggagaaaaag
     9961 attatattac aaattacaaa gatagtggtt atagaagcta tcatgttata ataaaatacc
    10021 ctataaactc cattgcgggt tcaaaggaaa ttatatgtga aatacaaata agaactcttg
    10081 caatgaattt ctgggctaca atagaacatt cattaaaata taaatatgac cattacatac
    10141 ctgagactct tgctgaaagg cttagaagag catctgatgc tgcctttctt ttagaccaag
    10201 aaatgagtga aataagagag gacataatga aagctcaggc tatgtaccaa atgaagtcta
    10261 tagctattag agatactcta aatagaatac aagagttata tgacttgggt gatacacata
    10321 aagctatgca gtaccaaaga aaacttgata gaactgaaac agataaaaat ataactgaaa
    10381 ttttggaatt aaaacaagaa atagatatat tattagagca atataaggat actaaggtag
    10441 atgataaagt agttcagtaa aaactacttg tagaaaattt gctaaatatt aatggggctg
    10501 cctcttaatg gaaaataatt tctaaaaaga gacagcctat ttagtgtaat taagaaagga
    10561 aggatattat gagtttgtat gctataggtg atttacattt ttcaacatca gtcaataagc
    10621 caatgaatat ctttggaagt aattgggatg gtcatgaaaa gaaaattata gataattgga
    10681 aagaagttgt taaggaagaa gatatggtct tagtactagg agatacatct tggggaatta
    10741 atttatccga agctaaaaaa gatttagata taataagcaa acttccaggt cagaaaatac
    10801 ttataaaagg aaatcatgat tactggtgga caaccgttac aagtttaaac aagctatatg
    10861 aagacatgcg ttttattcag acaaattttt atgaatataa agattatgca atttgtggag
    10921 gtaggggctg gatatgtcct aatgatgtca aatttgatga gactgatgaa aaagtttata
    10981 aaagagaaga acatagattt agactatcat tagaatctgc tcgtaaaagt ggtcactcaa
    11041 aaataattgt gattactcat tatcctccta cgaatgataa attggaagaa tctttattta
    11101 caaagttatt tgaagaatat aatgttgaaa aggtgattta tggtcacttg catggtaaag
    11161 aatcttttaa aatgggattg aaggggattc gaaatggagt agaatataca ttagcatctt
    11221 gtgactatac agaatttaat ttaataaaag ttcacgatta aaaaacataa attggtaaat
    11281 actataagat acatgattaa aaataaatat ttgagggatt tcggttttga gaaaggagac
    11341 acgacttatg aggtggatat tagtttttat atttttatat cttattcctt tagtagtgat
    11401 attcagaaat tacaagaatt ttaagaggtc ttgtatctac agtagtattt atgtggtatt
    11461 agctacaaca ataatgataa gtaatattta tatgagtgga cttaataaaa taaaagagtc
    11521 tatgtattat cacaattata tttctgatga gacatatgat gaaaagtatg tatcaaattt
    11581 taaggaaaaa gaaagtactg atttaaaaca ggataataaa aagaatgaag aaatatcaaa
    11641 taataaaaat ttaaacaaca aacaagatag aaatatacaa aataaagaaa gtaatgaaga
    11701 aggaagtaat gatataaaag aaaagaataa ttctgatagt aatgtttcag tgaataataa
    11761 atcagaaaaa caagaagata atacaaaaga aaataataaa tatacaagta aagaaaaaaa
    11821 tagtgaagat ataaaaaaaa tagatatgga aagtatagga atttttaaga aagaaattta
    11881 taatatagaa agagcagctt tagttccaat gagagaatgt ataccttaca ctaaggatat
    11941 agcaaaaaat ataactaaat tatctagtat aaaaaaagat gttacaaatg ccagaaacaa
    12001 gtgtaaagaa gttgtaaaag aatataaaaa tatgcagatt ccagaacttt ctaaggagaa
    12061 gtatacagaa acattgagta atgctagaga tgacgtaaag aaagcatatg agcttagaga
    12121 aaaatctatg gaaagtgctt tgaaattaat aaatactaaa agccctaaat atatagggaa
    12181 gataacagaa tatcttgatt tatcagataa gcagatagaa agttttaaaa atagattgaa
    12241 cgatttaaaa gaagaaataa ataattcaga aacaaataat attaattgat aagatttaag
    12301 gatataactt aaaagggaga ttatgatgaa taaacagagt ataggaaaac taaatatgtt
    12361 ttttatgggg attactcaaa gaaaccttga taatagggat ttttttattg agttagaggt
    12421 gatttttaaa tctggaagaa aagagtttaa aggaattgca atagaggaag ataataaata
    12481 taaatttaat tttaaaggaa aatcagattt atatacgttt aatgagttgc ttaagaatat
    12541 ttctaaagag gcagaaaatt atgatggatt agtatttaaa tatgttgagc gaggaactat
    12601 tgttgtaatt gaaggagaca ataagaaggt aaatgtaaaa tatttagata ataaggaaga
    12661 agtacctaaa atagatgaat ttacggcatc tcaaataaaa aacagagatt attatgtaaa
    12721 agtaggacaa gcaaatgcac ttttaaaaga aattggagta cttacaaaag atggtaagat
    12781 aaaaaatgat aaaataagaa aatacaatca gatagaccat tttgttgaat tgatagatag
    12841 tattttaaaa gaaataaagg ataaagactg tataactatc ttggattgtg cttgtggaaa
    12901 atcttactta agctttgtct taaattttta tataaaagaa gtattgaaga aaaactgtta
    12961 ttttatagga atagattatt cagatgtagt tatagaagca tcaaaaaata tggctaaaaa
    13021 cttgggttat aaaaatatgt catttataaa agaggatttg actaattata cacctaatag
    13081 ggatgttgac cttgtaataa gtctacatgc ttgtgataca gctacagata tggcaatagg
    13141 tctaggaatt agagcaaaat ctgaagctat agtagtagta ccatgttgtc ataaggagtt
    13201 attagggcaa tatagatatg aagctatgga accaatatta aaacatggtg tatttaaagc
    13261 tagatttgca gatttgataa ctgatggttt aagaacattg ttattggagg gcaatggata
    13321 tgatacttct gtagtagaat acatatctcc acttgataca ccaaaaaatt taatgataag
    13381 agcgataaaa actaaaacga ataatgacaa agcattaaaa gaatataaag aactgaaatc
    13441 acaatttgga gtggaaccaa ccttagaaaa acttatatat tagttaaaaa tgtaaaattt
    13501 acgaaaagct tgcttaaata tgcaagcttt ttggtattat ataggtatag tcatatccag
    13561 agttttgatt tataattaaa gctgtgctat aatatttgat taatcaaatc aataaatgtt
    13621 acatggaggt aaaaatgaac aaattgaata tatgtattga tatagatgga acaataacaa
    13681 gcccttatca ttttcttcca tacctaaatg atatctttaa taaaaatata actgaagcag
    13741 agtgtataac acataattgg gaagaattgt atggttcggg aataagagat gtgtatgcgg
    13801 agtttaataa taagtatata cactcatatg atgaagcaaa gattgtagag ggtgctacag
    13861 aggtaattag cacattatct aaaggacata atttatcttt tgtaacggct agacatgagt
    13921 gtttaactga tgtaactaaa aattggctat cgagacaagg attttcagat atagatgttt
    13981 acttattagg aagtgattat aaagttgaaa aggcaagaga actgagttgt gacatattta
    14041 tagaagataa tcctttaaac tcagtgcaac tagctgatga tggtgttaaa gtaatacttc
    14101 tggatactaa ctataataaa gatgttaaac atcaaaatat agttagagtt agtaattgga
    14161 tagatattga aaagattata aaacaaggta tttaaagatt tgtagattga aagggggagc
    14221 atatgataga tttaaaaatt gaaaatagaa aaggaaaaat ccatgtaaag agcagtgaaa
    14281 taaaagacat tttagaatta cgtccagact tcgaatatgt acaagatata tcaaatacta
    14341 taaatcaaga aaatatattg gtatttgatt gccagttgac aggaaattct tttgatatgg
    14401 atgatttaga tatagaagaa atattggaag agttaggtga agaaatagat gagtcttatt
    14461 tcagtgtttt atttgaagat gtaagagcgt atttaaaaga tgctactgat gagatagaag
    14521 cagacttaca ggataattac ttatttgata atttgagatg ttattttgat atatacaata
    14581 taaatcaaga atttacagat tttaagtttg tatttttagt ttcttttaaa gatataaaaa
    14641 tttcttcatt agcaaaccta gctaagattg tttctaagag acagcttata ggtgcatcta
    14701 agttttatag ttaaaatgaa atattattat aataaaagct agtaaccttg taaaaatata
    14761 ggttactagt tttttatttg ttcattttca caaaatttca gaaaactatt tacattctaa
    14821 aaaaagtaat gtatattaaa cataatgtac attataatgg ataaaaagtt tctattaaat
    14881 tactaagttt atttaataat caaaataatt tatcttatat tagtatgtat tagggggtat
    14941 ttatgcaaag tttaaatgaa ataattgctg aaaatttaaa aaagataagg aaagaaaaac
    15001 atcttagttt agacaagatt gcacaactta gtggtgtaag taagagtatg ctatctcaaa
    15061 ttgaaaagtg tgaggtaaat cctactatat ctactttgaa gaaaataact aatggactaa
    15121 aaatctcatt tacatcattg atggagaggc aggaaagtga catagaattg attcaaaaaa
    15181 gtgatataga ccattttatt gaagataatg gaaagtatat aagttatcca atatttccat
    15241 ttgactccaa aagaagattt gagatattta tgattgaaat agaagaaggt ggaaatttag
    15301 attcaaatgc tgaacttcca ggaacacaag agtttataac tgtttttagt ggcgaagtaa
    15361 ccattaaaat aaatggagaa gattatattg tatctagtgg aaattctata agatttaaag
    15421 ctgatgtatc acatatatat agaaactcag gcaaaggtat agctaaaatg agtatggtag
    15481 tatattatat ataaataatt taatggaatt agagtgatat ttagatgatt ttaggtatga
    15541 agatatatta tatttaagtt ataggagtgt gatttatgga tgaataataa aaaagcagtt
    15601 tttttctttt gtagtgattt aaagaaagac ccagtagcca gtaatgtgat aaagtgttgt
    15661 gaagaaataa tggatttaaa tcaaactgat attgttattg atgataattt tgtattagag
    15721 tttaaggata aaaacaataa cttatttcat tttgtaaaaa caaaagatgt aataagtcat
    15781 aattataaat attatcttcc aattttaaat agacatttta gagattatga ttttgcagga
    15841 gttgtaaatt ggcatgaggg agaaaatgct ccagaacata tcctaacagc tcatacaatt
    15901 ggagatgttc caacaggaga atttggaaat tcaaatccaa gatactttaa aaatctaatc
    15961 aatgctattg aggatataaa aaatgaaaat ctacttgatg aatttactac attaacagaa
    16021 gcaactcact ggtctggaac aacatatggt gaagaatcta aattaattac agaatattct
    16081 gtacctatgt tggatattga gataggaagt agtagtgata gttttaataa tagtatagca
    16141 atacaggtat tggcaaaatc attaattaga gtatttgatt gtgatgaacc tttgaagact
    16201 ttgttatgtg ttggaggggt acattttgag aaatctttct cagatattat aaaaaataaa
    16261 gaatataaca tatcaattgg acatgtattg ccaaatcaat ggattgtaag tggaatgtat
    16321 gatgatgaat ctggatttga gaaactagaa aagtgtataa atagtattga aggaggaatt
    16381 gattgtatag tttttcatga taaattaaaa ggtacctata aagagcaatg ccgtaaacta
    16441 ggtgaaaaac ttaatgtacc tgtatttaaa cataaaatat taaaaaatcc aaaagactta
    16501 cctatatggt agttttatac agttaatata acaataagtc ggattaattt ttaagcgagg
    16561 tcaaaaatga tattatctta ttttgcatta ttaattagag tagtgctatt agcttttgaa
    16621 agagtagttg taagattatt aggtgatgaa gaaggagata tttataaaaa tatagcttca
    16681 tcatttctat ttttctttat agggggagta tgtttacttc catttagcat aatggataga
    16741 gttagtgatt ggtcgttttt aataccatgt tatataagta gtttagtcta ttcaattggt
    16801 tcagtagcgt atgtaacgtc tttagcaact ggggaagttt cacttgttac tccaataaat
    16861 agtttaaatt cattgttttt actgctttta tcagttatat ttttaggtga aggtttaagt
    16921 ttagcaaaag ttgcaggaat cataattatg attggtggag tgtttatatt aaaaaaagta
    16981 agctcttttt taaagaatgt ctctgtaatt tttaacaatt taccatgtag attaatgttt
    17041 ttatacatat ttttgcagtc agtaggaaga gtattggata aggctttttt tgtacaagta
    17101 tctcctatat tatattcaac tatactgtat tttttcgtag gtttaaattt atttatcttt
    17161 ttagcattta aaaagaaaca aaaaataatt tgtgagattt ttactaataa aaaggggctt
    17221 tcaattttaa gtggtgctat taatggatat tcttatttag cattattaat tgctttaaat
    17281 aatctagaac ttagtatagc agagccattt tctcaagtat ctatgataat aactttaata
    17341 cttgctcatt ttatattcaa agaaaatata aaagaaaaaa taccaggttc aattttgata
    17401 ctaattggtg ggtggctact gttactttaa ttgacataaa aaatgaatta taaaggtgct
    17461 attagttagt atcagaaaat ttaaaaaagt attgacaatt cttttaggca aatgttaata
    17521 taataataca aaatagtata aatagaagac atgttactgg taatgcaggc aggatcgaat
    17581 atattgcttt gatttttcaa ggtgtttata taggttctgc cttttttgca tcttgaatgg
    17641 ccattcataa aaaattatgg agaaggagat tacaagatga attataaaaa aactgccatt
    17701 gacattattg aacaagttgg tggttcaaaa aatattttag ctttagaaca ctgttcaact
    17761 agactacgct ttaagttgat tgatagaagt aaagtcaatc aggatgcttt aaaaaaagta
    17821 caaggtgtta tgagtatagt taatggggca caatttcaag ttgtcattgg aaatagtgtt
    17881 gttgatgtgt atgatgaaat tttaaaagtt tgccctttag atacaaatga tagtactgta
    17941 aaaactgaag aaaaaataaa cttaggaaca aaatttatag aatttattat taatattttt
    18001 caaccactga tttttgctat tgcaggagct ggtattttaa aatcactgtt gatgttatta
    18061 gcaatgttta atattttaaa atcagattct actatatata cattattagt tgctatttca
    18121 gatgcaacat tctattttct acctcttatg gtagctgtaa ctactgccaa tgtattgaaa
    18181 tgtaatcgtt tagtagctat tgccgctgta ggatacttat tgcttccagc aacaacaact
    18241 gctttaagtg aaggtgtcga actatttgga tttacaattc caaacattgc ctataatgca
    18301 caggttttcc cggctatctt atgtgtgtct ttcttggcaa caatggagaa aatttttaac
    18361 aagtattcac caaaaccaat tcgaactttc tttgttccta tgatgtcttt ggcaattact
    18421 gttccaatta ctctaactat tttaggtcct ttaggatata acgttggtac aatttttaca
    18481 accattattt tattcttata taacaaaatg ggattcttag ttgtaggatt acttgctgct
    18541 tttcttccat ttatggttgc aacaggaatg cacaaagcac taatacctta ttcaattaat
    18601 acaattggaa agttgggtta tgaagcttta tatatgacat cttcattagc ccacaatatt
    18661 tctgaaagtg gtgcttgttt tgcagtagcc ttgcgaacaa aagatgaaac attaaaacaa
    18721 acagcattat ctgcaggaat ttctgccttg atgggaatta cagagccagc tttatatggt
    18781 attactttac aacataaaag agcaatgtta ggtgttgtat tctcaagtgc aatttctggt
    18841 acatttttag gattatttgc agtaaaagga tttgtaattg ttggaccagg gcttgccagt
    18901 atgagtatgt ttattgaccc aaacaatgga aacaatttga tttttgcaag tattggtttt
    18961 gttattgcaa tattaggctc gtttattatt acactgatta tttggaagga agatacaaat
    19021 acagtagagt catcaattgg agatgttgtt gaagaagagc tagaagaaga aaaagaagta
    19081 aatatcttag taactccagt agaaggaaaa gtaatagatt taagtaaggt taatgatgaa
    19141 ctatttgcaa gtaagacttt gggagatgga gttgcaatta taccgaccaa tggaaattta
    19201 tacgcacctt gtgattcaga agttgtgatg ttatttgaaa caaaacatgc aattggttta
    19261 agaacaaaaa atggggctga gattttgatt catattggaa taaatactgt aagtatgaat
    19321 ggagatggat ttaaaacatt tgttaagaca ggcgataatg taaaagaagg agatttatta
    19381 attcagtttg atttagacaa aatctcacat gctaatttag attcaactgt tatgattgta
    19441 aacaataatg gagcagatta tgcttataaa gtgttaaacc agtcttatgg caatgtaaaa
    19501 aaaggaagta tcttgtttga tgtaaaggga ggaatataaa tatgagaaag tttccagaag
    19561 gatttctttg gggaggagct acagctgcta atcaatttga aggtggttgg aatttaggtg
    19621 gtaaagggtg gtctgtatca gatgtagcaa aggcacactt tgatgcagat gtgaaagatt
    19681 ataaatctaa taatgaaatc acaacaaaag atatagaaga aggattagca catcctgaag
    19741 atgaagtaaa ttatccaaaa cgtcatggta gtgattttta tcatcactat aaagaagata
    19801 ttgctctaat ggcagaaatg ggatttaaga catatcgaat gagtattgct tggtcccgta
    19861 tttttccaaa tggagatgat aaagagccaa atgaagaagg tttacagttt tatgatgatg
    19921 tatttgatga gttgataagt tatggaattg aaccacttgt tactatgtca cattatgaac
    19981 caccattaaa tatcgtttta aactatgatg gatggtattc acgacaagtt atcaatatgt
    20041 ttgttaggta tgtagaaact atctgtgaaa gatataaaaa caaagtgaaa tattggctta
    20101 catttaatga agttgattct atgattcgtc atccgtacac tactggtggt ttagttcgag
    20161 accgttttaa ggataaaaac tttgaggaag ttatttttca agctatgcat catcaatttg
    20221 tagctagtgc ccttgcaaca aaaatttgtc atgaaattat tccaaattca aaagtagggt
    20281 gtatgttaac aaaattaacg tattatccat acacatgtag accagaagat gttttggcaa
    20341 ctcaacgaaa aatgagaagt atttatgcat atagtgatac gcaggttttt ggtgagtatc
    20401 cagtatattt actgtcgtat tttaaaaata ataacattca aatagtaaaa gaagaacatg
    20461 atgatgaaat tatgaaaaag tatccagtag attttatttc attctcttac tatatgtctt
    20521 cttgtgaggc agctgataca actggattag atattacacc tgggaatacg ttattagctg
    20581 taaaaaatcc ttatttagaa atgagtgaat ggggttggca aattgattca ataggtctac
    20641 gtatatcttt gattgaatta tatgatagat acagaaagcc tttatttata gttgaaaatg
    20701 gattgggagc caaagatatt ttaactgaag ataaaaaagt tcatgaccaa tatagaatta
    20761 attatttaaa agaacacttt aaatgtatgt tagatgcgat tattgaagat ggtgttgaac
    20821 tttggggata tacttcatgg ggttgtattg atttggtcag tgaatcaacg aaacaaatgt
    20881 caaaacgtta tggatatatt tatgtggatg ctgatgatta tggaaagggg acatataatc
    20941 gatataagaa agatagtttt tattggtata aaaaggtaat tgaaagtaat agtatagatt
    21001 ttaaatgacc aatagaaggt gagcaaataa aatgaactac ataataaaga aagttttgaa
    21061 ctcgagcgtt gttttagttc atgacacaaa ggataatgaa tttattttgc tagggaaagg
    21121 aattggatat ggtaaaaaaa caggagaata tatttgtggt tccaatgata atcaaatgtt
    21181 tgtccctgtt gaaaatacaa aatcaaaaca atttttagaa ttaatggatg atattcctgt
    21241 cgacatttta aagataacac aggaaataat agtagaggca gaaaagttat tgaactcatc
    21301 ttttaataaa aacttatact taatattagc agaccatttt aactttgcaa ttgaaaggat
    21361 gcgtaaagga ataaaaatta caaatcgagt attttgggaa ataaaaaatt attatccaaa
    21421 tgagtttaag gttggaatgg ttgcaattta tatggtagag gagagattgg gcattcaatt
    21481 accagaggaa gaagcagcta acattgcttt ccattttgca aatgctatgg cttcagatgg
    21541 aaattcatat gatacgatta agtatgccaa agtaattgga gaaataatta atatttttgt
    21601 tttttctttg aatagaacct tagataaaaa gagtatgcat tatatgagat ttataacaca
    21661 tataaaattt tttgtggaaa gatttttttc agatagtatg ttaagcagtg gtgatgattt
    21721 attatttaat caaatgaaaa ggtcaaatcc aaaagaaatg ttaattgcat taaaagttag
    21781 agatttttta gaaaagaaat atggaaaaaa gttaacaaat gaggaaatag ctttcttggt
    21841 tgtccatatc gcacgagtta agcaatagag tgaattacta atattatctt atacgtctac
    21901 atggtagaaa a
//
LOCUS       DAEMIK010000065        21816 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEMIK010000065 DAEMIK010000000
VERSION     DAEMIK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.065"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            603..2273
                     /locus_tag="KPK87_002024"
     CDS             603..2273
                     /locus_tag="KPK87_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG2538174.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRINYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQTQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2395..3129)
                     /gene="pflA"
                     /locus_tag="KPK87_002025"
     CDS             complement(2395..3129)
                     /gene="pflA"
                     /locus_tag="KPK87_002025"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG2538175.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3289..5520)
                     /gene="pflB"
                     /locus_tag="KPK87_002026"
     CDS             complement(3289..5520)
                     /gene="pflB"
                     /locus_tag="KPK87_002026"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG2538176.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5813..6874
                     /locus_tag="KPK87_002027"
     CDS             5813..6874
                     /locus_tag="KPK87_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG2538177.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            6965..8578
                     /locus_tag="KPK87_002028"
     CDS             6965..8578
                     /locus_tag="KPK87_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2538178.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8853..10838
                     /locus_tag="KPK87_002029"
     CDS             8853..10838
                     /locus_tag="KPK87_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2538179.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            10843..11277
                     /locus_tag="KPK87_002030"
     CDS             10843..11277
                     /locus_tag="KPK87_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG2538180.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11306..11866
                     /gene="srlA"
                     /locus_tag="KPK87_002031"
     CDS             11306..11866
                     /gene="srlA"
                     /locus_tag="KPK87_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG2538181.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            11930..12298
                     /locus_tag="KPK87_002032"
     CDS             11930..12298
                     /locus_tag="KPK87_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG2538182.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12323..12889
                     /locus_tag="KPK87_002033"
     CDS             12323..12889
                     /locus_tag="KPK87_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG2538183.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            12930..13295
                     /locus_tag="KPK87_002034"
     CDS             12930..13295
                     /locus_tag="KPK87_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG2538184.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13396..14169
                     /gene="srlD"
                     /locus_tag="KPK87_002035"
     CDS             13396..14169
                     /gene="srlD"
                     /locus_tag="KPK87_002035"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG2538185.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14518..16662
                     /locus_tag="KPK87_002036"
     CDS             14518..16662
                     /locus_tag="KPK87_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2538186.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16750..17085
                     /gene="spoIIAA"
                     /locus_tag="KPK87_002037"
     CDS             16750..17085
                     /gene="spoIIAA"
                     /locus_tag="KPK87_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG2538187.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17099..17527
                     /locus_tag="KPK87_002038"
     CDS             17099..17527
                     /locus_tag="KPK87_002038"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG2538188.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17550..18314
                     /gene="sigF"
                     /locus_tag="KPK87_002039"
     CDS             17550..18314
                     /gene="sigF"
                     /locus_tag="KPK87_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG2538189.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18594..19031
                     /gene="spoVAC"
                     /locus_tag="KPK87_002040"
     CDS             18594..19031
                     /gene="spoVAC"
                     /locus_tag="KPK87_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG2538190.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19198..20211
                     /gene="spoVAD"
                     /locus_tag="KPK87_002041"
     CDS             19198..20211
                     /gene="spoVAD"
                     /locus_tag="KPK87_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG2538191.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20225..20587
                     /gene="spoVAE"
                     /locus_tag="KPK87_002042"
     CDS             20225..20587
                     /gene="spoVAE"
                     /locus_tag="KPK87_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG2538192.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            20770..21786
                     /locus_tag="KPK87_002043"
     CDS             20770..21786
                     /locus_tag="KPK87_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2538193.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         8491 a         2452 c         3810 g         7063 t
ORIGIN      
        1 atataccctg taagcaactc tattttcatg tactaaaaat gtacctattt tattaactga
       61 aaagaataaa ttaaatgtaa gtacataaga agatagaaaa aattaaaaac aaaaaatatt
      121 tatataaata aatataaaaa atgatataat ccctattaaa aaatttatag atgatgaaga
      181 aatatcaaaa tcttatatta taggaagaga cacttctttg ccaaaatcta tagaaaagca
      241 ataccaaatc caaagagaat aggcggagaa aaaaataaaa taaattcaag agtaatagaa
      301 aagttttata gaataaagaa ctgagaactc tttatataat gaaaaataga attttaagag
      361 aaaatgattt aataaatgat ttaacatggt gttttagcag gtaaaaataa atcaccagta
      421 atgattatag aaaaattact ttatgaatct caaaaaactt agttaagagt aagagatttg
      481 ataagataac taaagttggg gaaatgtgtt tgatgagata aaaaattagt aaaatagtat
      541 aatctaaata aggatatttt gttgattaaa ctacactaat agtaatattg tagggggaaa
      601 agatgagaga agaatcgaac tctttaagtt tagaccatat agaaattttc tttgaacttt
      661 tatctcaaag tacagaagat tatatatttt tttgggatat aaataggaat aaatttaaaa
      721 tttcttcagc aatttttgat gaatttaact taagtaaaga gatagaatct gatttggtca
      781 attgttggag taaaattgtt tatccggatg atgttcaaat atggaaagat gatatacaag
      841 aattactaca tggaaaaaaa ggtgagcaca atttagaata taggttgatt aataaatatg
      901 aagaaatagt atggatttct tgtagaggta aagtttatgt gtcggatgac ccaaaaacta
      961 tatttctagt tgggagaata aagaatattg gtgaaaaaaa taaatttgat tctatcacag
     1021 gaacgtggaa tagagaacaa tttgaacata gaataaatta tcttataaaa gagaaaattt
     1081 ataaaaatgg tgctatgttt atcatggata ttgataattt taaaaatata aatgagaaat
     1141 atggacattc ttatggtgac aaagtattgc gtgcaattgc tactgaagta ttagaatatc
     1201 ttccaaaaga tgtaagattg tataggttag atggagatga atttgcattt ttttacccta
     1261 tgtgtactaa agaaactata gagaaaatat atgaaaagat acagatgtat acaaatactc
     1321 agcatgaaat tgaatcaaat aagtactatt gcacagtgac tgcaggagtt gctatgtatc
     1381 cagaagatgg agataattat ttagatttat ttaaacatgc tgatattgca ttggatatag
     1441 caaaaattag tggtaaaaat agaattaagt ttttttcaca agagctctat gaaaataaac
     1501 taaaggtaat ttcaatgcaa caaaaactaa gagagtgcgt tgaaaataat tttaatgatt
     1561 ttgagttgtt ttttcaaacc caggtaaatg ctgttacaaa agaggtaata ggggcagaag
     1621 tacttttaag atggcattca agtacttatg gagaagtatc tcctgttgaa tttataccaa
     1681 tactagaaca gagtaattta attataccag ttgggaagtg gattataaaa gaagctgtaa
     1741 aacagtgtaa agaatggcat aaaataaatc cagactttaa aatatctgta aatgtatcgt
     1801 atattcagtt aaaggaagat ttttttagag attttatagt agaatgttta gtagagtatc
     1861 aattaagacc tgaattttta attttggaat taacagagaa ttgttggata ccagatataa
     1921 atttactaaa tgataaattt attagtttaa agggtatagg agtatatatt gcgatagatg
     1981 actttggtac aggatattca tctttaaatt accttaaaga actgtctgtt aatataataa
     2041 aaattgaaag aagttttgtt aagaatatta catataatag ttatgaatat acttttcttg
     2101 aatatattat taaattagct catattataa atttaaaagt ttgtgttgag ggaatagagt
     2161 cttatgaaga gtatgatata gtaaaaagct taggtgtaga tattattcag ggttttttat
     2221 ttggaagacc tgtaagtgcc tctgaatttt ataaatttca actatcaaag tgatgtatat
     2281 aaaatgagat atatagtata taaaaatagg gcaaagtgga agttttgaag tttattaaaa
     2341 atttacactt taccctattt tgttatgtct tctataaagg attaatatac tacactaact
     2401 attatatact ttcactccaa atttagagat tatttcacta gctttttgga catcttcctt
     2461 agtggcatca ggaacatctt ttagttcata atcgatacct aaactttccc atttgtgtat
     2521 tcccatagaa tggtatggta gcaattcaaa tctttctaca tttttcaatg tagctacaaa
     2581 ttgacctagt ttttctaaat tatctatatc atcagtaatg ccaggaacta aaacatgtct
     2641 tatccataca ggaatatttc ttgattctaa gtgtctagca agttttaatg ccttatccat
     2701 accaacacca gtcaaatcaa tagatttttc ctcaaccata tgttttaaat caagtaaaac
     2761 caaatcagta ttatctaaaa ctgggtctat agtttctata tctacaaatc cagatgtatc
     2821 caagcaagtg tgtatatcat tttcttttgc ttttttaaat aattcactta aaaattctgg
     2881 ttgaagagtg gattcaccac cagaggcagt tatacctcca ccagaaaact tcataaaaga
     2941 agtatactta agagcatccg ttattatttc atcaactgtg tattcttttc ctgatttaat
     3001 atcccatgta tccctgttat gacagtattt gcatctaaga ggacacccct gaaaaaataa
     3061 tatatatctt attcctggtc catctacagt accaaacgtt tctatagaat gtatctttcc
     3121 cttaatcata gagttcacct cacaatatat tttaataacc ttatcaatag ttataatatt
     3181 tctctaaaaa aggccatctt ataccagaaa taattcaaat ataagatagc cttttatttt
     3241 tattttttac acagtagtaa tcttagttaa agtataaaaa tttaaagact aagccatttt
     3301 accgtggaat gttctgttta taacatctaa ttgttgctct ttagttaatt ttatgaagtt
     3361 aactgcatat ccagatactc ttatagttaa ttgtggatat tcttctggat gttccatagc
     3421 atcttctaaa gtagctctat caaatacatt tacatttaag tgatgagcat tttgagcgaa
     3481 gtatccatcc atcattgcag aaagattgtt tattctttca gtcatatctt ttcctaaagc
     3541 agcaggtact atagagaaag tattagatat accatcttga gcatgttcat atggtaattt
     3601 agcaactgaa gataatgaag ctaaagcacc attagtatct ctaccatgca ttgggttagc
     3661 accaggagca aatggagcac cagctcttct accatctggt gtattaccag ttttcttacc
     3721 ataaacaaca tttgaagtta ttgtaagtat agattgagtt ggataagaat ttctataagt
     3781 tttattcttt cttattttat tcataaagct ttcaactaac tctacagcta tactatcaac
     3841 tctgtcatca ttatttccgt attttggata atctccttct atttcaaaat caacagctat
     3901 tccttgttca tttctaatag ttttaacctt agcatgtttt atagcagata atgagtctgc
     3961 acaaactgat aacccagcca taccacaagc catagttcta aatatatctc tatcatgtaa
     4021 tgccatttct aatgcttcat aagaatattt atcatgcata taatgtatta catttaaagt
     4081 atttacatat aatgttgcta accaatcagt gaatggttca aatttactca taacttcatc
     4141 ataatctaag tactcagaag ttataggttc aaatcttggt ccaacttgaa caccagattt
     4201 ctcatctaca ccaccattta tagcgtataa taaagtttta gctaagttaa ctctagctcc
     4261 aaagaactgc atttgtttac caattctcat tgcagataca caacaagcta taccatagtc
     4321 atctccccag tatgctctca ttaaatcatc attttcatac tgtactgaac ttgtatctat
     4381 agatacttta gaacagaaat ctttgaatcc ttgaggtaat ttagttgacc atagaactgt
     4441 taagtttggt tctggagaag gccctaatgt atatagagta tttaacattc taaatgagtt
     4501 tttagtaact aatgttctac catctattcc catacctcct atacactcag ttacccaagt
     4561 tgggtctcca gagaataggt tattgtaatc aggagttctt aggaacttaa ccattcttaa
     4621 tttcataaca aaatggtcca tatactcttg taattcttct tctgttacga ttccagcttt
     4681 taaatctctt tcaaagtata tatctaagaa agtagatgtt ctacctaaag acattgcagc
     4741 accattttgg tctttaacag cacctaaata accaaagtat aaccattgta cagcttcttt
     4801 tgagttagtt gctggttttg atatatcaaa tccataagat tcagccattt ttttaagttc
     4861 atttaaagct gatatttgct cagttatttc ttctcttaat ctgataactt cctcatccat
     4921 gcaagaaact tctaatgatt tcttttgctc atttttatct tctattaaag catcaacacc
     4981 atatagagca actcttctgt agtcaccaat tattctacct ctaccataag catctggaag
     5041 acctgttatt ataccagatt ttctagcagc tctcatttct ggagtataaa cgtcaaaaac
     5101 tccttggttg tgtgtttttc tgtatttagt aaatatatct tttatttctg ggtctaattc
     5161 atatccaaaa gcttcgcatg aattttcaac catctttata ccaccatatg gcatgacagc
     5221 tctttttaaa ggggcatccg tttgtaaccc aactattgtt tctaattctt tatctaaata
     5281 tcctggagca tatgcagcta ttccagaaac agtttttgta tcaacatcta aagtaccacc
     5341 attttctctt tcttttttaa ataggaccat tgcttcgtcc cataattttt tagtattttc
     5401 agtggcacca gctaagaaag agtcatttcc ctcatatgga gaatagttta attgtataaa
     5461 ctctctaaca tttatttctt tagtccatct tcctgtttta aatccttgcc atgcattcat
     5521 taagtatttc ctccttagat ttaaatgata aataatacta ttaaaattaa tttttatata
     5581 cttaatttat tttatatatt tttttaaaaa tatattttct aatataataa tagcatatta
     5641 ttcgtatcct gtatacataa aaaataaaaa attgtcgctt taaagtataa aacagattat
     5701 atgttaataa tgtggtgaaa ttatacccat atataaataa aataaaacaa ataaatagct
     5761 aattaaggag aaaatatttt ttattttttg tttaggataa ataaatttta ttatggaaaa
     5821 aagaaaaagc atacatcttc acaaactgaa tatttattta atagggggag gtgatagatt
     5881 gtttatcaca gtggtattat ttttagtagg tttcctatta attactaaag gcgcagatat
     5941 ctttataaat tgtactgtag agataggtaa aaaaacgaat atatcagaaa ttatacttgg
     6001 agctactata gttagttttg caacaacatt acctgaattt acagtgtctc tacttgcttc
     6061 tattgatggt catactacta tgagtttagg aaatgcagtt ggttctataa tatgtaatac
     6121 agggttagca ttagggttgg ttgtttttat aagtccattt aatgtagata aaaaaatgtt
     6181 tttttctaag tctttactat taatagtatc cgttatagtt ttaattttat tgagtttaga
     6241 tggagttata acaagaggtg attcattatt acttataata atattaatct tttatatggt
     6301 taataattat agaagtgtcg ttggaaaatc tgatactaaa aatagaaata ttaaaaataa
     6361 cactattaaa gacatatcta ctaaaaaagg gaaaaattat agaggttttt ccgtattaga
     6421 aattgtaaaa atactattac tttttgctac aggattgata atgatgataa taggttcaca
     6481 aatccttata gagagtggag taataatagc tagtttttta aatatacctc aaggtatagt
     6541 aagtttaact ataatagcac ttggaacttc tctgccagag atagtttcgt cgattactgc
     6601 tataagaaaa aatcatcatg aaatatcagt aggaaatatt ttgggtgcaa acatattgaa
     6661 tatagtatca gtaatagctg tatcagcaat accaaataac ataccaatac tatctcaaaa
     6721 tagacaattg gatataccat tcatgatatt attattatta atagtaataa taccaacact
     6781 taaatctaat aaacttagta gaatacaagg tatattgatg ttattcacat attttctgta
     6841 catatcaatt ttgtatttca tgtatattat ttaactatac tgtttaaaat attaattgca
     6901 gaatatccta aaaaagtata taatggatag gttaagatta attgataacg gagaggaata
     6961 atatatgaat ataacaaagt ttgaagattt accaataagt gagggaataa aaaaagctat
     7021 agctgaaatg gggtttgaag aaccatctcc tatacaagca cagtctatac ctgcgatttt
     7081 gtcaggtaaa gacgttatag gacaagctca aactggtacg ggaaaaacag ctgcttttag
     7141 tatccctata ctagaaacaa tagacccaaa taatagaagt ttacaagctg tagtgctttg
     7201 tccaacaaga gaactggcaa tacaagtatc aacagaaata agaaagttag ctaagtattc
     7261 gcacggaata aagacattac ctatatatgg tggtcagcca atagatagac aaataaaatc
     7321 tctaaagagt ggtgtgcaag ttgtaatagg tacaccagga cgtacaatag accatatcaa
     7381 tcgtaaaaca ttaaagatgg ataatgtaaa aatgattata ttagatgaag cagatgaaat
     7441 gctagatatg ggatttagag aagatataga aatgatttta agtaagattc cagaagaaag
     7501 acaaacaact ttcttttcag ctactatgcc aagaggaatt ttggaattaa ctaagagata
     7561 tcaaaaagac ccagagcata taaaagttgt tagaaaagaa cttacagttt caaatacaaa
     7621 gcaatactat atagaaacta ggtcatcaaa taaattagaa gtattatgta gattagtaga
     7681 tgtatatgac cctaaactat ctgttgtttt ctgtaatact aagagaaaag cagacgaatt
     7741 ggtaggagat ttacaagcaa gaggatattt tgcagatgca ttacatggtg acttaaaaca
     7801 gactcaaaga gatatagtaa tggataagtt tagaaatggt acaatagata tacttgttgc
     7861 tactgatgtt gcagctagag gtatagatgt tgatgatgtt gaatgtgtat tcaactatga
     7921 cttaccacaa gatgaagagt attacgttca tagaattggt agaactggtc gtgctggtag
     7981 agaaggtatg tcatttactt ttgtatttgg aaaagaaatg agaaagatga aagatataga
     8041 aagatatact aaatctaaac tgataaagca taatatacca acaataactg atgttgaaga
     8101 gaaaaaagta ggtactttct ttgcacaagt taaacaaact atagaagaag gtcacttaac
     8161 taaacaatta caatggttag aaggtttttg taatgatgaa gattatgcaa tggttgatat
     8221 agctgctgca cttgtaaaac tatctctagg tgaagaaatg aaagaagaaa taatagaaga
     8281 aaaacctaga agagagcgtg gagatagaaa aggtggtact ggagctaaag atggtatgat
     8341 tagattattt atcaatattg gtagaaatca aagagtacaa gcaaaagata tagttggtgc
     8401 catagctgga gaagttggaa taccaggtaa agtagtaggt actatagata tatatgataa
     8461 gtatactttt gttgaaatac ctaaaaaaga tgcaaaaaca gtaattgaaa agatgaaaga
     8521 tattaagata aaaggtaata agattaatat agaaaaagca aataagaaga aaaaataacg
     8581 tatagtatac aaaactataa ttagaaaata tataaaataa tatattggat atttaaaaag
     8641 cctgatgtgt tttcaaaaca gatagtagat aatctgtaag agttaacagc ataaggcttt
     8701 tcttgtttta tatgatgtta taattttcca gattgttgtg aaaaatgtat attagttcct
     8761 ttttttcaaa tctgatttga aaaagagaat attaattatc tatatagttt agattataat
     8821 aaaaaatgta agataacaaa ggggagaaaa agatgtcaat gacttatatg gaccctaggg
     8881 tttttttgat tttagaaatg gtgttagaag aagacacaaa tactctagaa caattttcaa
     8941 atgttttggg tgttagtact aggactatca gaaactatat aaaccagatt aatcgagaat
     9001 taaaaggtgg tattgctaaa atagttaaaa atgaaaaagg tacatatagt ataagaattg
     9061 aggatgaaag taaattaatt gagattgtaa attttaatag aggaaagcta tcaaatttta
     9121 taaatttaaa ttcacctgat gaaagaataa attatgttct tgatatttta actatgacca
     9181 acaatccaat taccatagat gatttggctg aagagatatg tataggaaga acaacattaa
     9241 taaaggattt aaaaaaagca gacagaattt tagctgaata tgatttagaa ttaaaaagga
     9301 agccaaatat aggtatgatt ttagttggtg aagaaatgga tataagatta ttaatccttg
     9361 atagattgta tgaaaattat attgatgtct tggaaaacgt taatcagata aatttagtta
     9421 aagattatga tattgaatgt cttagagatg aattaaaaaa gttatttaaa aaagaagaac
     9481 tctacataac agaacaggtc ttgagagatg ttgaaagata tataatcatg tctgtcttaa
     9541 gaaatctaaa tgggtacaaa tttgaaaaga tagataagag atttgaagtg ataagatgtt
     9601 cagatgaata tactttagga ttaaagctca aagctttatt ggaaaagata tttaatatag
     9661 ttttaaatga aaaagaaacc atatttttga caatgccatt aataggtaga aaagctccta
     9721 gtacaaaatt agcacttagc agtataaaga taaatgatag cgtaaaggaa gttgtagatg
     9781 aagtaactag ttttttatta gagacctcag ggattgattt taaagaagat aaaaagctta
     9841 tagaaaattt ggaatatcac ttgtattttg cacttaatag aatgagattc aacataaggg
     9901 taaaaaatcc tcttttacaa gaaataaagg aaagatatac atttccatat agtgttgcaa
     9961 agatagctgc aatgataatt gaggaaaagt ttgacttgaa aatatgtgac cacgaaatag
    10021 gatatatagc attacatttt ggaagttatt ttgaaaaaaa ttcaagaaaa gttctaaatg
    10081 taaatagggt agctgttgtt tgtggaacag gtcttgggac tgcacaactt attagaataa
    10141 agataggaaa aatacttgga gaaagtgtag aagtaaatac tttttctgat atagaagcta
    10201 aagctaattt agatttatta aagtattatg atttagtatt tacaacagta gatttcaaaa
    10261 cagacttaga tgttccaatt tttagagtaa atgcgttatt taatgaagaa agtataaaga
    10321 aagagataga aaatgcaata cttttgaaaa atagtataaa tcttaagtct ataaaaaaga
    10381 ttgaaaaaaa tatccctttt ataggtaaat taattgaaga ggataagttc tttatattga
    10441 ataagagtac gtttatggaa aatttagaag atatgttaga aaaattagtt cataaaggaa
    10501 ttattgatat agagttcaaa aaaaaggtat taaaaagaga aaagaaatct tcaacagcat
    10561 tagataatta tatagcattg cctcactcag ttaatactaa tggggaaaat ctttatctgg
    10621 catttggaat attagaaaaa ccagtgattt gggatacaaa ggaaattaga ataataatat
    10681 tgatgataat tccagataag aatgtagata gtacagacct gataataaaa agttatgaag
    10741 aagtacttga agtagggaga gataaaaaga tggtagaaga acttgctaag gcaaaatcat
    10801 ttgatgaatt ttataaaata attaccaaga ggaggtaata atatggaccc agtaattttt
    10861 attatagcat cacttattac agtgtattta ttaaatattt ttcttggata cttacagctt
    10921 aaagatttta ataaaaatta tattgaacta aaaagaaaag gaagagtggc aattggcaga
    10981 aaaaaaggaa gaatttcatc gggtacaatt gtgttgatac ttataaatga tgatggtgtg
    11041 attgtagaga ctagaaaaat gcaaggtgtt acagtattgg caagagtaaa gtcatttgaa
    11101 ggtttggtag gaaagagctt agagtatata gaaaaatcag atttaactgt ttacaataaa
    11161 ctacttaaaa aagcaatact agatgcagtc aaacagtaca taacatttga gaaaaataaa
    11221 tgtgaagata caaataatct gagtactgat gaagagtgta ggatggaagt agtttaaaaa
    11281 aatatattta taaggaggag ttaatatgac gcaatttttt caagtattag caagtggtgc
    11341 agatagtttt atgaatttat ttagggcagg tgggcaacaa tttgtaggtt ttgtaacaga
    11401 tatagtacca cttttagtag cattattagt tacaatgaat gcaattatca actttattgg
    11461 tacagataga gtcgagagac ttgcaaaaaa atgttctgca aatatattta ctagatattt
    11521 gatattacca gtccttggta catttgtatt tgcaaaccca atgacactat cattaggaag
    11581 attcttgcca gaaaagttta aacctagtta ctttgcagca gcaagctttt catgccatac
    11641 aatgaatgga ttattcccac atatcaatcc aggtgaacta tttatatatc taggaattgc
    11701 aaatggtata acaactttgg gattctcaac agcagattta gcagttagat atttgttagt
    11761 gggtatagtt gcaaacttta ttaaaggtgt agttactgac tatacaacaa aatttgtaga
    11821 gaaacaacaa ggaatagcat taaattcaga tataaagatt ggataattac tttaaggtat
    11881 aaataaattt ttcaaataaa aagatagtgt atagaggagg ataaattata tggcatttaa
    11941 caaagttaaa atagtaaaag gttctggagg atggggtgga ccattgataa tagaaccaac
    12001 agaaaagaag aataaggtag tatatgtgac tgggggagca aagccagaaa ctgcagtaaa
    12061 aatagctgaa cttacagggt gtgagttaat agatggattt tcacagggtg ttaaagatga
    12121 tgaaatcgca tgtgtaataa taaattgtgg tggaacattg agatgtggta tatatcctca
    12181 aaagaaaata ccgactataa atataatgaa aacaggaaaa agtggaccat tagcaatgtt
    12241 tataaaagaa gatatatatg tttcaggggt aaaaccaaca aatgtagagc agttataatt
    12301 gaggtagtag gagggaagaa atatggctta tgatactaaa aagaagataa gtgagcagag
    12361 tcaaggaatt gttgcaaaaa taggtatagc actaggaaat gtgacaagta ctctttttca
    12421 agctggtagg gatactatag atacattaat acatactata ctgcctttta tggctttcgt
    12481 tgcagtccta ataggtataa taaatcaatc aggtatagga aattgggtag ccaatttact
    12541 tactccatta atcggaaatg ttggaggttt agttgtctta tcaataatat gttcatttcc
    12601 gttgttgtct ccgtttcttg gaccaggagc tgttatagct cagatagtgg gagtattggt
    12661 tggtacagaa ataggaaaag gaaatattcc accaagttta gcattaccag cattgtttgc
    12721 aataaactca caagcagctt gtgactttat accagtaggt cttggtcttg cagaagctga
    12781 acctgataca gttcaagtag gagttccgtc agttttatat tctagatttt taacaggtgc
    12841 accaactgtt ctattagcat ggttagcaag ctttgggtta tataaataga agaatacttt
    12901 ataaataatt agagagaggt tcagttgata tggaattaat atacagtact caaataaata
    12961 aaataggaga taatgcaaat gaatttttac aacataatat gtttgtaact ttcaaagata
    13021 atgctcctca agagttagaa aattactgtt atatacattc tgaaaataat ttagtaaaag
    13081 aaatacttac ggatgatgtg ctatcaataa ataatgttga ttacaaaatt acttctgttg
    13141 gtgagttagt aaaccaaaat ctttcagaat taggtcatat aacatttaag tttacaggtg
    13201 aaaaagatgg agcaattgga ggaaccttat atcttgaaga aaaagaaata gcacctattg
    13261 gcgtaggtac tatactaaaa ataattagaa aataaaattt aaaacgaatc taatcatatt
    13321 ataagtaaat tatagataaa agtcaattta actaatcaag ttaactttta atgtgataaa
    13381 aaaggggaaa tgaaaatgag aaaagtggct ttagtcgttg gaggaggaag aagtttaggt
    13441 gaatatttat ctaaacattt agctaaagta ggttatgatg ttgctattgc agatttaaat
    13501 gaagacaacg caaaaaaagt agctgaagaa ataaaaactg aatttggttg taaatctgta
    13561 ggatatagtt gtaatgccat agatgaaaga caggttataa gtactgtcaa agctattgaa
    13621 aatgattttg atagaataga tttattagtg tacaatgcag gagtggctgt aagtaaaaaa
    13681 ataactgatt ttggatacaa agattttaaa tttattgtag acgtaaattt aaatgggtac
    13741 tttctatttg caagagaagt atctaaagtc atgatagcaa aaggtattga gggtagaata
    13801 atacagataa attcaaaatc aggtagagtg ggaagcaagc ataattcagg atattcagca
    13861 ggtaaatttg ctggagtagg acttactcaa agtttagcgc tagatttggc agaacataag
    13921 ataactgtaa atgcactgat gctcggtaat ttacttgatt ctgatatgtt tgaaagttta
    13981 attcctcaat atgcagtaaa attgggaata cctgaatcag aagtcaagca agtttatata
    14041 gataaagtac ctttaaaaag agggtgtaga tttatagatg ttgcaaatat gcttacattt
    14101 tatgcttcaa aggaagctga atactgcact ggtcaatcta taaatattac tggtggacaa
    14161 gttatgtaag gtttttatgg agtgtctcaa gatgaactct ttagttcatg aggcactttt
    14221 ttgtataaaa ttaaatatat tttcagcatt taaacaatga aaaagaggtt tatctttatt
    14281 ttgagatagc ctcatttttg taattaaaaa acatagtgtt taagtaaaca tataagattg
    14341 ttagtttttt aaaagagaat tttttaataa tattatattt atgaagtatt tttaaactta
    14401 ttttgataaa atatagctat gttttatata ctgtagtttt gaacatagaa agtttaataa
    14461 attatagtat atatgttaat ttttaataag gtaagaaagt agaaagaagg atattttatg
    14521 gatataataa aaaaattaag tcatgggtgt acattagatt gtcatgactg ttgtaaattc
    14581 aatgtttata ctaaaggaaa taatgtagta aaaattgaag gagataaaaa tcatccttat
    14641 acaaaaggtt ttatatgcaa aaaagggatg gcccatttag atagattaaa tcataaagat
    14701 agaataaaga ctcctatgct taaagttgat ggagtatgga aagagatttc ttttgataaa
    14761 gctatagaaa taatggcaga aaagcttaca tactataaag aaaagtatac atcaaagtct
    14821 gtcatgcatt atgaccaata tggaagtggg tctgtgctaa aatacatagg agatatattt
    14881 tttaattttt atggaggtgt tagcagacat aaggggggtc cctgttggag tgcagggatg
    14941 catgctcaaa aatatgattt tggagtagct aagagtcatg ctatagaaga tatgttaaat
    15001 agtaaaagta tatttgtttg gggaaaaaat ccagcatata ctacaattca tactatgcaa
    15061 ataattaaaa aagctaaaga aaaagggata aagatagtag tgattgaccc catatataca
    15121 aaaacagctc aaatagcaga taagtatgta caagtaaatc ctggaacaga tggagcttta
    15181 gctatagcta tggcaaaaat tatagtagaa gataaattat atgatgaaga atatataaac
    15241 tcgtatgtta ttggttttga agaatataaa aaatatttat cttcattgga attaagtttt
    15301 ttaattgatg aatgtggtgt taaagagaat gatataagag agttagtgga tttatatact
    15361 aacaaatatt caagtataaa tgttggatat gggcttcaaa aatataaaaa tggtggaaac
    15421 acaataagag caatagatgc tttaggtgct ataactggtc aaataggatt tagtggtgga
    15481 ggggtaaact atgctaataa agtttatccg agtgttataa attctgaccc ttacaatagc
    15541 caatcatatg gtgaagatag ggagttttat gtaagtaata tcagtaaatt tatagaagaa
    15601 tctttaaaaa atacatcaaa taaagtaaat tatgcatcag atgaacttga tatgacatca
    15661 aataaagtaa attatgtatc agatgaactt gatataacat caaataaaac agattatata
    15721 tcaaatgaat tgtataattt atctaataag tcaatcaaag gtaatattcc tataaagatg
    15781 gctgtaataa ctaaaagtaa tatgctgaat caactgccag atttagtaga attggagagg
    15841 gtgttttcaa aaattgaatt taaagtatgt tttgatatgt ttataactga tacagctact
    15901 ttatgtgaca tgtttatacc gtgtacaaat acactagaga gtgaagatat aatttatagt
    15961 tcaatgacca atccatatat aacctataat gaaagagctg taaagccagc acataaattg
    16021 atggatgaat attacttctt tatggagcta gctaaaaaga tgggattaaa tgattatcct
    16081 tttgtagaaa agagaactta tttagaaaaa gtgattgaac cacttaagcg ttttgacaaa
    16141 aatctggata tagaaaaact taaaaacaat tactttacta tacataaccc tgttgcttgg
    16201 gaagataaga agtttgagac tccatctgga aaatacgagc tttattcaga aagtataaaa
    16261 aacttgggaa taagtccaac acctgtatat ataagcaata aatataaaga aatagaagat
    16321 aaaaatattt cttttagatt attaactaat catcatgcag atactttatt tagtcagcat
    16381 tttatggaca aaaaatctat agctcaagca tatataaatc ggagaatggc taaaaaagta
    16441 ggtattgagg acaaagatat tgtgatattg aggtcaaaaa aagcaaaaat aaatgtacaa
    16501 ataaatatag atgatggagt aggtaattat atagtaaaaa tgtatgttgg ttggtggaag
    16561 aaacatggaa atcctaatag tttaacagac actggtatat cagattttgg gggtcaagta
    16621 acatataatg aaagtatggt agaaataata cgacaaaact agttaaattt cattgaagga
    16681 ataaaaatat aattatagaa ttgattaaaa gttactattt ttacaaaact attgaaggag
    16741 ggatttttga tggtaaatta ttccttagaa cataagaatc tatacataga atttatgtgc
    16801 tcagaactag accaccatgt agccaatgaa ataagggaag aaatagataa cttattaagt
    16861 gtaaatcaag taaaaaatgt tgtatttaat tttgaaaata ttaattttat ggattcttca
    16921 ggtataggcg tcatcatagg tagatataaa aagatttcta atgaaggggg acgagtttca
    16981 gtaattaata taagttcacg agttaagaag atttttgatt tatctgggtt aaataaaatt
    17041 attggcattt atgatacgta tgaagaagct ctaagttctt tgtagggagg agtatgtgat
    17101 gaataatatt atggaagtta aattttcggc aatatctgaa aatgaaagtt tagcaagagt
    17161 tatagtagca tcctttgcag ctaagttaga cccaacagta gatgagttag tagatataaa
    17221 aactgctgtc tctgaggctg ttacgaatgc tataatacat ggatatgaag aagacagttc
    17281 taagtttgtt ttcttaagat gtgagataga aggaaatact ataaagatta tagttgaaga
    17341 tgaagggtat ggaatagaaa atgtggagaa ggcaatggaa ccactttata catcaaaacc
    17401 agaacttgat agatcaggca tgggattcac agtaatgaaa agctttatgg acgatgtaga
    17461 ggtaagttct gtaaaagata atgggactag aattgaaatg actaaaaaaa taaatgtgcc
    17521 taaataaagt ccaaaagtga ggttttatta tggaagtaac tgttgccaga gaagaaaaaa
    17581 aacctcttct tagtcatgaa gagactctag aacttattga aaaagttcaa aatggagatg
    17641 aagaggcaaa agaaatatta atatcaagca atttaggtct agttaggagc gttgtatcta
    17701 gatttttaaa tataggatat gatagagaag atttatttca gttaggttct ataggcttaa
    17761 taaaatcaat atataaattt gacccaaaat ttaatgtaaa attctctact tatgcagtac
    17821 caatgatttt aggtgaaata aagagatact taagagatga tggtatgata aaagtatcaa
    17881 ggtcgttaaa gcaaatagct gtaaaggcaa agatggaaag tgaagcttta actaaaaaat
    17941 taggtagaga gccgagtata gaagaattag ctaaagcaat agatgtagaa aaagaagatt
    18001 tagtaatggc tatggaagct aatttcaatg ttgagtattt gcaaggagtt attcatgaag
    18061 aggaaggttc tccaatatgc cttattgata aaataagcat gaaaggagaa agtgaagaag
    18121 aaaaagtagt ggataatata ttgttaaaag acatacttgg aagactggat aagagagagc
    18181 gtcaaataat agttctcaga tactttgaag acatgactca aagtgaaata ggagagatgc
    18241 tcaatatatc acaagttcaa gtatctagga tagagaagaa agtattgtct aagttaaaag
    18301 aatatatatc gtgataatac ttatgatatg tagaataaca aaatataata aatatattac
    18361 taaaataaaa tgtaataact ttttatttta agtttttaaa ttattaaaag atattaaaat
    18421 aataaaaagc attaaaaaag taaaaatata aaaacaaggt gtctttatgg aaaaatagat
    18481 tttttcttta ttaggcatct tttttatttt ttgaccaaac tttagatagt caaaataaat
    18541 aataggttta tttttttaaa attaacaaat aatactttag agaggtgttt agtatggata
    18601 aaaattataa aaaatatgta gaccaaataa gcccaaaacc tacatattta aaaaactata
    18661 cacttgcatt tattgtaggt ggaattattt gtatgatagg gcaagcaata aatgatttgt
    18721 atatgaaagt tggaggtctt gataaactag gtgctagttc tgcaacatca ataacattaa
    18781 tatttatagg ggcattttta actggattag gagtctacga cctaatagga aaaagagctg
    18841 gagctggttc tataattcca ataacaggtt ttgctaactc aatagtatct ccagcaatgg
    18901 agtataaaag agaaggctat gtattaggtg taggtgcaaa cttatttaaa attgcaggac
    18961 cagttttagt ttatgggata ggttcttcta ttttatgtgg aattatttat tatattttta
    19021 agatgtttta gatgattttt atttaattta aattttatta attatgaaga aggtataaaa
    19081 tctgaaaata gctactaaag taactaattg aaaaataatt gtgcagattt aattaatatt
    19141 tttaattaat attaaaaatt tgaatagtaa aatgcaaaat taaaaaggtg tgaaaatatg
    19201 aaaaataaaa gaattggaaa aagaacagtc aagttagaaa ataaaccaac cataatatca
    19261 actggaacta tagtaggtcc taaggaaggt gaaggaccac taaaggacta ttttgatatg
    19321 attatgactg acgatttata tggtgaaaag acttgggaat tagctgaaag taaaatggta
    19381 gagactgcat ctcaacaagc tatccaaaag gcaggtaaaa aattgtcaga tgtaaattat
    19441 atgctaggtg gcgatttaat aaatcaaata gtgccagcat catttgcagc aagagaactg
    19501 gctattccat ttttaggtat atatggagct tgttctacaa tggcagaagg tctttgtata
    19561 ggttctatgc ttatagatgg tggttttgct gatttagtac tgtcaggtac atcaagtcac
    19621 tattgtacag cagaaagaca atttagattc ccacttgaac ttggaaatca aaaaccaatg
    19681 acagcccagt ggacagttac aggagcagga agtgtattat tagctcctaa tggagacggt
    19741 ccaaaagtaa agtatgttac tgttggtaaa gtaatagatg agggtattga tgatggtaat
    19801 aatatgggac cagctatggc tccagcagca atagatacta tctattctta ctttgaagat
    19861 actaatgatg atccaaatag ctttgatata attgctactg gagatttagg tactcttgga
    19921 aaacagatag cagaagactt tttaaaagaa aagggtgtag atatatcaaa agtttacact
    19981 gactgtggta tagagatgtt taaccttaaa gaacaagatg tccattgtgg tggaagtgga
    20041 tgtggatgtt cagctacagt atttgcagga tatatatatg ataagttgag aaaaaaagaa
    20101 tttaacaaag taatgcttgt atcaacagga gcactacttt caccaacaag tacactacaa
    20161 aagcagacaa taccatgtgt tgcacatgca gtagtaatag taaatgagta ggaagtgata
    20221 taatatgtta atggactatg taagagtatt tattgtaggt ggaattatat gtttaatagc
    20281 ccaagtaatg atggattatt tcaaaatgca aactccatat gttatggtaa cgtatgtaac
    20341 aactggtgta gttttaactt ttctaggact ttatgagcca ttagttaagt ttgcagcagc
    20401 aggtgcaaca gttcctataa taggatttgg atattcactt gctacaggag taataaaatc
    20461 aattcaaagt gatggttttt taggtatttt tttaggaggt acaactgcta cagctggtgg
    20521 aatagcagca gctatattct ttggatatat tatgtcagta gtatttactc caaaagcaaa
    20581 accataaatc tttattaaaa gaataataag tttttgaaat aataaatttt tataaagcag
    20641 gtggatatct tttagattcc acctattttt tatataaaga agcgtttaca taattgaagt
    20701 tgacaattat ttacaacata gtgtagtatt atattatcaa aataaaatga agtgaagtag
    20761 gggataaaga tggtaactat taaagatatt gcaaggaatc taggtatcag ttattcaact
    20821 gtttctaggt gcttaaacaa taatcctaat gtatctgaaa aaacaaaaaa gaaagtggta
    20881 gaagaggcaa atagactagg gttccatttt aatgtaaatg caagaaattt agcaaaaaaa
    20941 gaaactaaca ggataggggt aatcttttca aataatttta atcatcaaga tactagaaag
    21001 ttttttagtg atataatgga tagttctata aattctattg agactaataa atacgatttt
    21061 ataatacagc ctaataataa cattagtggt gatagtaatg tatataaaat ggtaaatgga
    21121 caaatggttg acggattagt aattgtgtct caatcaataa aaaaagaaga gtatgatttt
    21181 ttaaaagaca ataattttcc acatgtattt atatatttta aaccatcttt tataggagaa
    21241 gtagacaatt tcttttggga cgataacgtg tatggaggtt atatagctac caagcattta
    21301 attgaccatg gtcataaaga tataataaca gtaacttcag atgataagag ttcaaaaatg
    21361 catgaagata gaaccaaggg atatttgaat gctatggaag aggcaaattt aaagacagaa
    21421 gtcattaaat ctaaaatgga ttttgaatct caagttgaat ttttagaaaa atacattgat
    21481 aaaataaaaa aagcttcagc tatatttgta caacaagatg taccagcgat ttctataata
    21541 caagagttaa aaactactta tggtataaat gtaccagaag atatttccat aatagggtat
    21601 aataatattg aattaatatc atattttagg tcgcatttat caacaataga tgacccaaga
    21661 gagcaagtta taaaaaatgg agtagatagt ttagtaaata taataaataa aaagagtaca
    21721 gaacacattc caagaaaatt atatccaagg ttaataatac gaaatagcgt taaaagaata
    21781 aagtagtaag cttttaagta tcaaaagttt attaaa
//
LOCUS       DAEMIK010000066        21559 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEMIK010000066 DAEMIK010000000
VERSION     DAEMIK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.066"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            270..1541
                     /gene="serS"
                     /locus_tag="KPK87_002044"
     CDS             270..1541
                     /gene="serS"
                     /locus_tag="KPK87_002044"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG2538194.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRAYMGVSVIK"
     gene            1954..3459
                     /locus_tag="KPK87_002045"
     CDS             1954..3459
                     /locus_tag="KPK87_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2538195.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3573..4271
                     /locus_tag="KPK87_002046"
     CDS             3573..4271
                     /locus_tag="KPK87_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2538196.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4556..5644
                     /locus_tag="KPK87_002047"
     CDS             4556..5644
                     /locus_tag="KPK87_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538197.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTAVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5883..7073
                     /locus_tag="KPK87_002048"
     CDS             5883..7073
                     /locus_tag="KPK87_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2538198.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIAHGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7164..8666
                     /locus_tag="KPK87_002049"
     CDS             7164..8666
                     /locus_tag="KPK87_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2538199.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFTFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8738..9994
                     /locus_tag="KPK87_002050"
     CDS             8738..9994
                     /locus_tag="KPK87_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG2538200.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10166..12940
                     /locus_tag="KPK87_002051"
     CDS             10166..12940
                     /locus_tag="KPK87_002051"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG2538201.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEENLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(13005..13190)
                     /locus_tag="KPK87_002052"
     CDS             complement(13005..13190)
                     /locus_tag="KPK87_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2538202.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTYEAIT
                     MEDCVQINKKLNMKMVI"
     gene            13565..14743
                     /locus_tag="KPK87_002053"
     CDS             13565..14743
                     /locus_tag="KPK87_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2538203.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      14870..14955
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            15073..17991
                     /locus_tag="KPK87_002054"
     CDS             15073..17991
                     /locus_tag="KPK87_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2538204.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSKIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYTFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      18177..18262
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18362..19024
                     /gene="zmp1"
                     /locus_tag="KPK87_002055"
     CDS             18362..19024
                     /gene="zmp1"
                     /locus_tag="KPK87_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG2538205.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(19146..20108)
                     /locus_tag="KPK87_002056"
     CDS             complement(19146..20108)
                     /locus_tag="KPK87_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2538206.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20278..21468)
                     /locus_tag="KPK87_002057"
     CDS             complement(20278..21468)
                     /locus_tag="KPK87_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2538207.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         8532 a         2491 c         3690 g         6846 t
ORIGIN      
        1 ataaagaaaa agtcaaaata gataattgat tagcaaaata gatataatta taatgaagtt
       61 aaatctagca atattctcct aaaattaaat ttcatttatt taattaaata taatttgaca
      121 atgggcaaaa aagtgagtaa aatataaata aaataaaaat aacaggttcg gataaagaga
      181 aaggtttctt aacctcaagc ataaagtata ttatctaagt tatttgagat taagagataa
      241 atccaatctt aggtactgag gaggaaaaaa tgcttgatat aaaaagaata agagaaaatt
      301 tagattgtat aaaaaaagca atggaaagaa ggggcgaaaa agactttaac cttgatgaag
      361 tagttaagtt agatgatgaa agaagaaaga ttcttcaaga agttgaagtt atgaaaaatg
      421 aattaaatac agcatctaaa aacataccaa atcttataaa agaaggtaaa gatgtagaaa
      481 atgaaaagat taaactaaaa gagttatctg ataaaattaa ggtaatagac caaaatctta
      541 aagaagtaga agataagatg gaatacctat taatgagaat accaaatgta ccacatccag
      601 aagtaccaca aggtgaaact gatgaagata atgttgaagt aagaacttgg ggaaagacta
      661 ctacctttga ttttgaatct aaggctcatt gggaaattgg aacagaatta ggtatacttg
      721 attttgaaac agctagtaaa ataactggtt caagatttac tctttacaaa ggattaggtg
      781 ctagattaga gagagcatta ttaaattttt acttagacac gaatacaaaa gtaaatggat
      841 atacagaagt tattccacca tttatggcaa atagaaatag ttttttaggt acaggacagt
      901 tacctaagtt tgaagaagat atgtttaaaa tagaaggtct ggactatttt atgattccaa
      961 catcagaagt tcctttaact aatatacatg ctaatgaaat acttaaattt gaacaattac
     1021 caataaatta tacagcttat actccatgtt ttaggtcaga agctggttct gctggaagag
     1081 atacaagagg tttagtaaga cagcatcagt ttaacaaagt agaaatggtt aaaatagttg
     1141 caccagaaga atcttacaat gagttagaaa agcttactaa caatgcggaa actatgctac
     1201 aattattgaa tctgccttat agagtagtaa agatatgtac gggtgattta ggatttacgg
     1261 cttcttttaa atatgatgta gaagtttgga tgccaagtta caataggtat gttgagattt
     1321 catcttgttc aaactgcgaa gatttccaag ctagaagagc aggaataaga tttaaaagag
     1381 ataaagattc taaagctgaa tatgtacaca cattaaatgg ctctgggtta gctattggaa
     1441 gaagtgttgc agctatatta gaaaattatc aacaagaaga tggttctgtg gttgttcctg
     1501 aagttttaag agcatatatg ggagtttctg ttattaaata agagttaaat tatgtatttt
     1561 taagcaatac aataatatag aggtaataaa aaataattaa tataaaagtg atacttctaa
     1621 tatagaagta ataaaaaaat agttaatgta aaagtgatac aataatatag aagtaataaa
     1681 aaatagttaa tgtaaaagtg atacaataat atagaagtaa taaaaaatag ttaatataaa
     1741 atcgatacaa taatatagaa gtaataaaaa aatagttaat ataaaagtga tacaataata
     1801 atagaagtaa taaaaaaaaa taattaatat aaaaatgtgt acaaactaat gaatattgat
     1861 atagtaagct atcatatgaa agagtatgta cacatttttt ttactaattt ttcacaatat
     1921 gaacataaat aatttacaag aaaggaatct tttatgaaaa ttcaaaatac tgagtgggga
     1981 tatattgaat ggaaacatac ttatgatgaa aataatccaa aacaagctat gaatatatac
     2041 attgcagtaa ctatgcctgg taagaaacat tttaatcatg ttcattatgg tcaagagcag
     2101 atgatatata ttttagaggg agagggtttg tatattataa atggagtttg gaagccattt
     2161 tatcaaggta tgatttttta tatagaatct ggtagcacac atgaaactat aaatacaggt
     2221 gatagagaaa taaaagaact tattgtttca aataatgtag atgatgtagg agagagtgaa
     2281 gttatagata ttaatcctaa taattattta aagaagacct tgataaatta tagtgaaagt
     2341 acattaaatt tgtatgcagc agtagaatca attcgtggtc aatttataga cccgtttaaa
     2401 attccattaa ttatatatga tgattcatgg aatatagttt taaaaaatcc ttattttcca
     2461 cttttttgtt ttgaaaaatg taatcctatg aaatttcctc aaaattgtga ttgtatgaat
     2521 caaaaaagta gcaatcaatt tgtatgtgaa tatgggataa ctatatataa tataccaata
     2581 ctgtacaaaa gcaatagcat aggtgttata agaggagggt atgtactatt atctgatttg
     2641 aatttagata cagagcataa taatttatat gatattccag agggagctgc tagaagtata
     2701 aaacgcctcc tgaaacaaat atctaaaaat ataataaatt tttgctcttt taatgatata
     2761 agaaaggatt tacaagaaaa agagaagact atagcaagaa catatcatta tggagagcaa
     2821 cttgaaatga atttaaaagt agctcaagat atggttacta atttaagaat aaatcatcac
     2881 tttttattta atacattaaa ttcaatggca agcatagcac tagatgatgg tagttatgat
     2941 ttgtatagtg caataataga tttatcaaga atgtttagat atacaatgag gtctgattta
     3001 agatttgtag aattagaatc tgaaatatta tacattaaaa attatttgaa tttacagaaa
     3061 ttacgttatg gagatgcact taaagtaaaa tatcttattc cagagaagtt atataacctt
     3121 tctgtgcctt ttaactttat tcaacctatt gtagagaatg catttacaca tggatttaga
     3181 gatattgaca cagaaaagcg aattgaaata attgcaagat tagattctca gtatgccatt
     3241 atagagattc ataataatgg aactatctta gatggaaata atatagacaa aataaaggca
     3301 ggtattcgta gtaataatgg tcatggtttg tctttaatat atacaaaatt cacatctgct
     3361 tatggaaata attttgatat ggatataaag tcgagtgata atgaaggaac ttacataatg
     3421 attaaaattc ctattgaaaa ttataaggag tatgtataga acttgtgaaa aattaataag
     3481 ttttataaat gttaagaaat atattagatg tttttatgtt gttttatata tcttactttt
     3541 ttatattatt aagctaaaag ggaggtattc tcatgagaag agcactaata atagatgatg
     3601 aagaagctgt tctaaaaata ataacacatt ttatagaaat gaaagatatg ccaataaaaa
     3661 ttgttggtaa agcaacatct ggtgatgaag cagtagataa aatcattggt ttagagccag
     3721 acatagtttt tatagatatt caaatgccaa tatacaacgg gttagaagta atagaaaaaa
     3781 catctcattt atctaaaaaa ataaactttg tagtaataac tgcatttaac tattttgaat
     3841 atgctcaaaa agctttgaga ttaaatgtaa aagatatact ccttaaacca ttggatatga
     3901 aagaatttac taagtctgta gaaaaaatta ttggatatca atataccaat aatgatttat
     3961 taaatgagat actggaatat ataaatctaa actatagcaa tgacttaaag ttaaatgatt
     4021 gtgctagatt atttttaacc aatccatcta atatatctag gatttttaaa agtaatttaa
     4081 atacaacttt tatatcatat ctaaatcata taagaataga aaaatctatt gagttacttg
     4141 aaaatactac aaaaagtata aatgagattg cagaaatagt tggatataat agcctaaata
     4201 acttttataa aaactttaag atagaaaaag gaatgactcc aaaagtttat aaactgaaca
     4261 ctaaaaattg aaattgataa ataaaaagtc aatatagata attgtttgag tgtgtctgaa
     4321 taagtatact ttaattatga taaaaattaa agcgctttaa acatatttaa taatattaaa
     4381 ccttataaaa gctgtaccta tttatataaa aaaatatata taaataaaaa gaaaaagata
     4441 tacaaaataa aaaaagtgaa ttaaatactg cttcaaaaag acagaatttt catttaattt
     4501 actgagagtt tatatttctt aaaggaaggt aactttataa tggaggtaat ttaatatgtc
     4561 agacaaagtg aatatgaaaa atattatctg tattgcaggc gcttatctag caacagcaat
     4621 tggttctggg tttgctactg gtcaagaaat tctacaattt tttgcctcac aagggtatat
     4681 gggtttaatt ggagcattaa tatcaatggt attgttttca tttcttggtg ctgaagttat
     4741 aactagaggt agagagttaa aacttaaaga accaacaaga atttacactt tttattgtgg
     4801 gaagatttta ggaaaatttt acgaatggtt tagtccaata tttttatttg ggattcttat
     4861 agtaatgatt tcaggtgcag gctcaacttt aactgaatac tatggtataa atccttactt
     4921 aggaagagta ttgatggctg ttgctacttt aattacagtg tctatgggtt tagaaaaatt
     4981 agtaagcata cttggacgta ttggaccagt gatagcagta tttacaattg ctgttggatt
     5041 aataagcttg tttaagcatt ttgatggttt atctatggtt ggagaagcta tgaaaaatat
     5101 tgaagttaca aaagcatcag aaagtccaat cgtttctggt gtaatatata atacattaaa
     5161 tattattgta atgattgttt ttttaacagg aatgggagct tctattgaaa gtaaaaagga
     5221 tgctttttgg ggaggtttat taggtggaat agtgtttatg actgctggta tagttatgta
     5281 tttggctatg atttcagata taggaaattt gtatatgaaa gaaataccat cactttattt
     5341 agcagataat atatctccta ttgtaggtgt atgtttttca gttgcattaa tattaggtat
     5401 ctacacaaca gctgttccac ttttatggtc tgtaaccaat agaattgttg aagatgaaca
     5461 tcctaaattt aaattggtaa cagcagtaat agctattttg gcttgtatag gaggtttttt
     5521 accgtttggt aaattagtaa acattttata tccatataca ggttatatgg ggcttttaat
     5581 tttagtctgt atggtgtata aagcaattac atgtagaaaa aaatcaacac aaagtcagga
     5641 atagagccac ttattaatag tttttattta aagaaatttt ttatataatc cgaatttaaa
     5701 aagtcaaaat agttaaataa attgcaaaac atgataatat atttaattaa aataatatag
     5761 taaaatataa atataaacta aaattaaatt tataaaaaat atctttataa agattattaa
     5821 cttatataat ttgtagttaa attataaaaa ataaaaaaat aaaattttgg agaggtgtta
     5881 gtatgcatga acttatttta atctcagaaa agtataaaga agatatttta gattttttta
     5941 cagatattca tatgcacccc gaattaagtt ttaaagaatt taggactaca aaagctataa
     6001 aagatttatt agtaagttta gacatagaaa tattagattt aggaatggaa acaggcgttg
     6061 ttggattatt aaaagggaag tatgatggtc ccacagtagc actaagagga gatattgatg
     6121 cattgccaat atatgaagag gttgatatta agtataaatc cagatatgat ggtataatgc
     6181 atgcatgtgg gcatgatata cacacgtctt gccttgtagg atgtgcatat gtattgtctc
     6241 atattagaga tagtcttcat ggaaatgtaa agtttatatt tcaaccagcc gaagaagtca
     6301 ataaaggtgc gaagatgcta gtggaacgag gagtaatgga aaatccaaaa gttgatgcta
     6361 tatttggact tcataatcat ccagatatac cttgtggaaa aataggtgtt aagttaggtg
     6421 ggttaatggc agcagtagac actattaaga tagaagtaaa tggatttgga ggtcacggag
     6481 gtatacccaa cagaacaata gaccctatag tagcatcaag tgcaattata atgggtattc
     6541 aaaccatagt aagcagaaac ataagtccat tagaatcagc tgttatatca ataggtacta
     6601 taaatggagg gactgcaaat aatgtaattt cagaaaaggt agatatgaca ggcacatgta
     6661 gaagctttag taatgaagta cgaaagaaaa tatcagaaaa tttagaaaat atagtttgtg
     6721 agatagctca tggatatcaa gcaactgcaa agctagatta tttatttgat ttaccagctg
     6781 ttataaatag taaagagatg tacgcaatag cttgtaagtc tgtgtgtgat ttgtattcag
     6841 aagatgctat agttgatcca ataccttcta caggaggaga agatttttct atattcatgg
     6901 aaaaagcccc aggatttttt tactggttag gggtaggcaa caaagaacaa gattgtatat
     6961 atcagtggca taatcctaaa tttaaagctg ataaaaattc tatattagta ggtacaaatg
     7021 tactatgtca gtctgttata aattatatgg ataaattaaa aaataaaatt taagatagag
     7081 cattctggag ataaagttta tgagggggta ttagtttgag gaaaattaat tcacaggaaa
     7141 aaaacaaaga tagtggatta ttgatgaggg cactaaatgt tgtagagaga gcagggaata
     7201 aacttccaga cccagtaacc atatttttat tactatgtat aattgttgta atattgtcag
     7261 cagttatatc taatttagga gtagaagaaa tacatccttc tacaaaagaa gttgtaaagg
     7321 tagtaaatct actagagaaa gagcagattc aatcatattt aggtagcata gttacaaact
     7381 ttcagtcatt tgctccttta ggattagtac tggttacaat gcttggagca ggtgttgctg
     7441 agaaatctgg atttatggaa gtgttaatga aaaagggtat aagtaaagtt ccacaaaaac
     7501 ttgtaactgt agcaatcgta tttgcaggta tgttgtcaca tacagcagca gatgtaggat
     7561 ttataatatt accaccactt gcagcacttg tgtttttagg aattggaaga catcctcttg
     7621 taggtatgtt tgcagctttt gcaggtgttg cagggggatt tgcagcaaat gttatgctta
     7681 gtactacaga tgtattgtta gctggattta caataccagc agctcaaatg atggacccat
     7741 cttaccaagg caatccagca atgaatcttt attttacttt tatatcagcg atagttttaa
     7801 cttttgcagg agcatttgta actgaaaaat ttatagctcc tcgttttaca aaatataatg
     7861 attcaacaaa tgataaggat atacaggaat tgagtgaact tgaaaataag ggtataaaat
     7921 acgctttatt atcattgtta gttgtagtag tagtgatagt tgccttatgt ataggtgaca
     7981 atgcatttat gaaagatcca gagactggtt ctatactatc tagtaatgca cctttaatga
     8041 aaggtatagt tcctataatc actattatat ttttaactcc aggattagtg tatggaaaag
     8101 taagtaagaa gataaaaagt gacaaagatt tagtatctat gatgggtagt agtatgagtg
     8161 atatgggtgg atatatagtc ctagcattta tagcatccca gtttattaat ttatttaatc
     8221 ttagtaactt aggaacaatt ttatcaataa caggagcaaa attactagca gagtcgggga
     8281 tacctagcta tggattgata ataggattta tattgctatc tggatttatc aatttatttg
     8341 taggaagtgc ttctgcaaaa tgggcgattt tagcacctat atttgttccc atgtttatgc
     8401 tactagactt taatccagca ttaactcaaa tagcttatcg tattggagat gcttcaacaa
     8461 atccaataag tccacttttt ccgtattttc cagttatatt ggcctttgcc agaagatatg
     8521 ataaagatat aggtataggt actgtcatat cgaatatgat tccatactca gtggtattta
     8581 cattaataga aataataata ttgttactat tcatgggtat tggcatacca ttaggacctg
     8641 gtggaggaat atcttacgtt ttatagaccg ttataataaa tttatcaatg aagattaaac
     8701 taataaaata tattctttaa ttaagaggag gattattatg ttaaaaacaa caaaaatttc
     8761 agacccagag ttatacaaga ttgtagctga tgagttggtt agacaagaac acaatattga
     8821 gatgatagct tctgaaagta cagcaccaac agaagtactt gaattaagtg gatgtgtatt
     8881 tacaaataaa acagaagaag gctatccagg ggctcgtttt caagcaggtt ctgaagaagc
     8941 agataaactt gaaaccctag caataaaacg tgcaaaagaa gtatttggag cagaacatgt
     9001 aaacgtacaa ccgtattcag gttctacagc taactattgt gtatattcat caatattaaa
     9061 accaaatgac actgttttaa gtatgcgtct tgaccaaggt ggacacttga cacatggtag
     9121 cgcagttaat tttttacatg acatttataa atatgaattt tatggggtag acccaaatac
     9181 tggacgtata gattatgatg ctcttgaagc aaaggcaaaa gaatgtagac caaaacttat
     9241 aattgcaggg gcaagttcat atccaagatt aatagattat gaaagaatat caaaggttgc
     9301 aaaagaagta ggagcttact ttatggtaga tatggcacat gttgcaggat tagtcgcagc
     9361 gaaagtaata cctagtcctg taccttatgc agattttgtt tcttcatcta cgacaaagac
     9421 tttctgtgga ccaagaagtg gtattgtact ttgtaaagca gaacatgcaa agaaactcga
     9481 taaaggtgtt tttccaggaa cattagggtc tatacatctt aatacagttg cagcaaaagc
     9541 cttttcacta ttatatttaa gtacagataa atttaaaaag ataatggagc aagttgtagt
     9601 aaatgctcag actctagctt ctgaactgat ttcacatggt tttagtattg taagtggagg
     9661 tactgataat catatagtaa tggttgactt aagaagtaag aatcttacag gaaaacaatt
     9721 tgaaaaagct cttgaatatg taggaataac agttaataaa aatgttatac ctgacgaccc
     9781 acaatcacca tttgtaacta gtggtgttcg tataggtctt acatcaatat ctcaaagagg
     9841 tctgaaagaa aaagaagtta tacagatagc agggataatg aataaggtag cagaaaatat
     9901 tgataataaa gaagtattag atgaatgtaa agcagaggca caagaactta tatctaagtt
     9961 tccattatat ccagaagggt attttgaaga ttaaatattt tgatttgtat agctcacaat
    10021 ttataaagcc tgtaacataa aatttaataa tatataatca ctataaggtg ttgattaatc
    10081 aacaccttat tttttttgaa aagtaatata caagctatca taagcatata attccttata
    10141 gataattaac aagggggata aagttatgag gtattataat aaacctacca aagaagtatt
    10201 aaaatactta aaaacaaatc cagaaattgg acttgatgat aatgaggtag aagaaaggaa
    10261 gcttagatat ggattaaatg aatttactat aaaagaaggt agaacatttt gggacgagtt
    10321 gggagaaagt ttgacagaac ctatgatatt aattctcata ggagcagcag taataagttc
    10381 ttttgtggga gaattacatg acgcattagg tattttaggg gctattttta ttggaatttc
    10441 tataggcata ataactgaag gaaaatctaa aaaagcagct catgcactgt caaaattaac
    10501 agaaaatata gaagtaaagg tacttagaaa tgggaagata attaaaatat caaaaaatga
    10561 tttagtacca ggagatattg tatatataga aactggggat atgataccag cagatggaag
    10621 acttatacag tctataaatt tgaaacttag agaagatatg ctaacaggag aatctgatga
    10681 tgtagctaag aatgctgatg ctgtattaga tatggaagta gtttattcta aaacagaaat
    10741 aatcgaacag gatgcaatac ctgcaaagca agtcaatatg gtatttggag ggactcttgt
    10801 agcatatggt aggggcataa tggttgtcac tcatactggt gataagacag agatgggtaa
    10861 gatagcacag aatttatcaa atgaagacca gcaaactcca cttcaaatta aattagggaa
    10921 gttgggtgct aaaatagcag gaatatcagg cataatagct actttattgt gtatgtttat
    10981 gattatacag atgcagagaa aaggtatgtt aattttagat acaagcagtg tattatcttt
    11041 cttacaatca ttagaacctg ctaaaaatgc atatatggtt tgtattgcat taatagttgc
    11101 aactgttcca gaaggtttgc caactatgat aaatattacc ttagctataa caatgcaaaa
    11161 gatggcaaag ataaatgcat tagtaacaaa gaaagaagcc tgtgaaacta taggttctgt
    11221 ttctgtaata tgttccgaca aaacagggac tttaacacaa aacaaaatga tggtagaggt
    11281 agcatatgtt gatggtaaat atataagtgg aggagagtat caaagtaata gctattttga
    11341 acaaaactgt atagtaaatt caactgcaga tatagaaaaa gaagataatt catttaaata
    11401 tataggaagc gcaacagagt gtgcattact tttatatcat aatgataaga actataatga
    11461 aatgagaaaa caaacgtatt tgatatcaca aataccattt agttcagaag aaaagaagat
    11521 gtcaacatta attcgtcaag aggattcaga tatattactt tcaaaaggtg ctccagaagt
    11581 attattaaaa aaatgttctt atgtacaaca aggaaaaaat attgttccaa taactccaaa
    11641 agtagaaaaa agtattttag atgagattaa gaagttacaa attaaatcta tgagaacatt
    11701 aggatttgct tataaaaaaa tgagtaattc taaaacagaa gttgcaatga cttcagaagg
    11761 tgaattgaat ttaataggaa attcaagaag ttatatgaag gaagaaaatt tagtttttag
    11821 tggatttgta ggaatagtgg acccattgag agaaggggtc aaagattcca ttgataaagc
    11881 atttaatgca ggagtagatg taaaaatgct tacaggagat aatataaata cagcaacagc
    11941 tattggaaat gaattaggat tattaaatga tgggaaaaag gcagttgaag caacatatat
    12001 agatgtattg acagataaag aattgagaga agaaatcaaa ggaatatcta ttgttgcaag
    12061 aagtaaacca gatacaaaaa tgagaatagt ttcagcactt caaaaaagtg gcgaagttgt
    12121 agctgtaaca ggtgatggta taaatgatgc cccagcactt agtcaagcag atgttggaat
    12181 tgcaatgggt atttcaggaa cagaagtatc taaaaatgca gcagatataa tattgactga
    12241 tgatagcttt agtactatag tagagggaat aaaatgggga agaggtatat atgagaactt
    12301 tcaaagattt atacagtttc agcttactgt aaatatagta gcatttataa ttgctataat
    12361 atctcaactt actggtaagg atatgccatt tactacaata caattattat gggtaaatat
    12421 aataatggat ggtcctccag ccctagcatt aggattagaa cctgttagag attatgtatt
    12481 gaaaagaaaa ccaataaata gacattctgg aataatagct aggtctatgt ttgtaaatat
    12541 tattataaat gctattctta taattacaat tgtatttact caatcagctt ttaatatatt
    12601 gggagctaca tcagaagaac aaggtactgt tatattttct ttatttgcat tcagtgccct
    12661 ttttaatgca ttaaattgta gagagtttgg tttaaatagt acaataccaa atttctttaa
    12721 aaataaactg gcattgcaaa taatagtagt tacagggata attcaaataa tatttacaca
    12781 agtgttccag agtttcttta attctgtttc tttggacttt gatatgtgga taaagataat
    12841 actatttgca tccactatcc tattgtcaaa tgaatttgta aagctaattc ttagaacaat
    12901 gaaaaatagt agaactatga actttaatag taaaaattaa ttcctatgaa accgcaaagt
    12961 actatatatt gtaactttgc ggtttttgtt tgtttaatgt tgttttatat taccattttc
    13021 atatttaatt ttttattaat ctgaacacaa tcttccattg taattgcttc atatgtgtca
    13081 acacattcaa agtcaatatc taatttttgt aatattttgt atgcctcttt ttgcaaagga
    13141 gtatcaaatt taataggtga ttttgtgtaa atatcactga taaaaaacat gatttatttc
    13201 attcacttcc atttaaaatc ttatagttat agtgtatcat aaggttataa aatataggta
    13261 aatatagtat aaatttaaaa tatgtacaga caaaacaaaa aaatcatatt ctaagtaata
    13321 cttgggaaaa atgatattat cataataaat aatctttgta aaaacttata tagaatctat
    13381 taaacagaca ctaagccata aaaactttac taaatataaa attcgtatct aaatagatat
    13441 atagaataat attttattga agtagagtga tttttaagaa ttttatatat aaattatgtt
    13501 atcataaata aagatataga ttataaaatt tgttaataaa ataaagggca gggaggtaag
    13561 tttcatgttt tccaaaatcc tagtcaaaac atttatcaga gatagcgaaa acgttcaaaa
    13621 tacagatgtt agaaataagt atggttatgt agctggtgta gtaggtatat tatccaattt
    13681 acttttattt gtgataaagg tattcatagg aatgctcaca tctagtatag ctataatggc
    13741 tgatgcattt aataatcttt ctgatatggc atcttcagca ataactatga taggttttaa
    13801 attagcaagt aagccagctg ataaagaaca cccttttggt catggaagaa tagagtattt
    13861 atcagcatta atagtagcat ttatggtgat gttggttgga cttcaatttg taaagtcatc
    13921 tatagaaagg atagtaaatc caattcctgt aaaatttgaa gtaataccac ttatactttt
    13981 aattgcatct ataatgatta aaatctggtt gagtcgtttt aataaattta tgggaaataa
    14041 aattgattct tcagccttaa aagcagtttc acttgatgct ttaggagatg tatttacttc
    14101 aagttgtgta gttatatcat ttattgtagc tagatttact aattttccca tagatggata
    14161 tgttggtatc gtagtatcat tagtgatact ttatgctggt ttctctcttg taaaagatac
    14221 tataaatcct cttttagggg aagcaccaga tgaagaaatg gtaaattcta ttatagagct
    14281 attgttatct tataaatata taataggtac acatgattta attattcata actatggagt
    14341 tggtagatgt attgcatcta tacatgcaga aattccatcc aacatagata taatggaaat
    14401 acatgaaatt atagatactg cagagagaga aatatctgaa aaattagata tatatctagt
    14461 aattcacatg gacccaatat gtcttgaaga taaagaagtt atgtcagcca aaaaagaact
    14521 tgaagaaata cttaagaaaa atagtcttgt aaaatctatg catgatttta gaatagttgg
    14581 aaaaggaagc aagaagaatt taatatttga tatagtagtt aatccttccg aattttccaa
    14641 aaacatgtca gaagatgact taaaagaaga tataacaaaa ttagttaagg agataaatcc
    14701 tgaatataat tgtgtaattg ttgtagataa ggattttatt taattattaa ttaatctcat
    14761 cataaggtaa aaaatgtaaa aaggatagac aatagtatag aggcataata taatagaagt
    14821 atataattgt atttttaata taaataaagc aattttaatt gtttttatga aatattatag
    14881 agatgttgaa gtatattcta ttattgggca ccttatggat atactgagtc agtggtgcaa
    14941 ccggctatga atataaattt atttattttc atagcttttt ttgtataatt ttttagtaaa
    15001 aattacttta tatacgatat taatggaaga ttagattata aattataggt aacctaaagg
    15061 ggtgagattt acatgaagaa aggaaataga aaggcattat taattagttt aattatgatt
    15121 ttaagtatgg tggtgtctac aatatatcca actgtatctt atgcttcaga attaggagag
    15181 aatagtcaga ttcaaagtgg ttcaactaat tcatcaactg gagaggagaa ggaaagcgac
    15241 aataaaaagc cagaacagac tccagagaaa gataaggcaa cagataataa aaaaccagaa
    15301 cagaccccag aggaagaaaa accaacagat aataaaaaac cagaacagac cccagaagaa
    15361 gataagtcaa cagataataa aaagccagag caaactccag aaggagaaaa gccaatagat
    15421 aataaaaagc cagagcagat accagaagaa gataagtcaa cagataataa aaagtcagaa
    15481 caagctttag aagatgaaaa accactagac aataaaaata cagaaaaaac tccagaggaa
    15541 gataatctat tagaagatga aaatttattg aaagttttag aagaagaact taatgaagaa
    15601 aatgaagact atggatttgt tgtaaaaatt aataacaata caatagaaac ggagtcaatg
    15661 aaaaaaatat catttaattt aacttatact ccaacatcaa aaggaataca agcaggagat
    15721 tcaataactt ttaaggtgcc agatgtattc aataaagtta atttagatta tacaagtgaa
    15781 tgctttgata aaacagaaag taatggagag tatacactta catttagaga attacctaat
    15841 ggccaaagtg taatgcaagg taaaataggt ttggaagcat atgttaaaaa ggttgatgaa
    15901 gatactaatg caaaaataca tattgaaact actggaaaaa tagaaagtgg aagtggagat
    15961 atagatgtag aaataaaacc tggagataaa acggatgttc cagatgctaa aggaacactt
    16021 aaaaagcttg ttgaaggaaa aaaatcaact actgtattta tgccagttaa aaataaagat
    16081 ataaattaca gtatacaagt aaatgaaaag caggaagagt taaaagacat tattttgtat
    16141 gatgagcttc ctgaaggatt gacattgata aatggttctg taagtgtagt aacaagtgat
    16201 ggaaaagaag ttagtgattt taatattgaa cagagtaaaa attctatttc agttaatttt
    16261 ggaaatatag ataaatcgta tactgttaag tataaggcta gaatcagtga caaaaatgca
    16321 aaacatggta ataaatataa gaatgtagct aggattgaat ctgatggtaa gaagatacaa
    16381 gaagatgatg ctactgtaag tatatttgat agaggagatg attatcttct tactaaagga
    16441 cattcaggag caacaaatat tactcaagtt ggtcaagtaa taaactatca gatttcaata
    16501 aatgatgata aatcaccaat aagtaatgtt gttataacag ataatatacc agaaggcatg
    16561 agattaacta cttcaggaga agctgggcat gattttagag ttgtagaagt tccaatgaat
    16621 gggagctgga ctccttggag taaagaaaaa attgccaata atatttcata taaagtagaa
    16681 gaaaaaagaa atgaaagtgg gcaagtagat aaagttataa ctggatttac aattaatctc
    16741 agtaaagaag aagtggaatc aaagtttttt atagcttata cattaaaggt aattagtatt
    16801 gaagatagct atataaatag agctgttctt gatgcaaata atagtaaaat tgataaaaat
    16861 gatgagataa actttaagaa aaattctgga ttaataagcg caaaaaaaga agttgataaa
    16921 aaagttttaa acagtagtga taaccagatt gttaaataca aaatcaatat gtcaacttat
    16981 ggagtttatg atgcaggaca agtaaatctt ttagatgaag taaactcagt tctagaaata
    17041 tctaatataa aatattctga taatcttgaa cttaaaaaag aagctggaga tggaaaaaat
    17101 acaataagat tagtaaataa atacgaattt aaacagataa aagaagggga acctgtgcaa
    17161 agttgggtta catttgatgc aaacttcaca aatgtaaaag ttggggagac tatacgaaat
    17221 gtggctcaga ttaatggttc aagtcctcca ggagtagaga ctactaagca aggttatact
    17281 tttgaagcta aaaaagtgga tgctttagat aaaaatgtac tttcaggtgc taagttcaat
    17341 ttagaagatg catttggaaa tatagtagtt aaggatttag ttagtgatga agatggtata
    17401 attcaatcta gtgtaaaaaa accaggaaca tattatttag tggagataat ggctccaagt
    17461 ggatatgaaa aactgaagga taaaattaag gttgaaattg gtaataaaga tattggtaga
    17521 atagtggata ttggaaatat agaaaacata agacaagaaa atccaccagt aaatccacca
    17581 gcaccaccaa atacagatga accaatagta aatccaccag taccaccaag cacagataaa
    17641 ccaaggaaac catcaagttc atctgataca gaggatacta tagttataaa tccaccagta
    17701 ccaccatcag aagatataat aaatccgcca ataccagagg tattgaatcc tccagtacca
    17761 ccatcagaag agatgataga aactcctgtt aaacagatta ttccaatacc agaagtagta
    17821 aagccatcag tgtcagaaga aaaaaataat aaagttaaag atgatacttt ggtaaatcca
    17881 ccagtaccac caaaaacagg ggatagtact acaattattg gagaaatact tttagttata
    17941 ggagctattg taggacttat cgtattaaga agaaataaaa atacaaatta gaccctaaca
    18001 ttataaaaaa tataagttat attaaaaaat atcttctaaa acaatattta ataatgaaaa
    18061 tattgttaga agatattttt ttttgaagat ttatgtaaaa aaataataac tttttagaaa
    18121 aaagttccaa agggatatag acaattaaaa agatataagc tataatatag atataaaatt
    18181 aaatgaggca aaactaggga aacctagtca cgcaaagcta taggggctaa agtttagtaa
    18241 accatgccag ccagttgccg aaaagagtaa aaatttcttt ttgtatatac aaagatactc
    18301 ttgggtatct tttttatttt gaaatttatt actcccggcc taactcaaag gagggaataa
    18361 tatgagacca agtaaaaaat tattaatagc tataatatca atattcttaa taagttcagt
    18421 tccagtaagt gcacatgcag atagtactac tatacaacaa aataaagaca cacttagtca
    18481 aatagtagtt tttccaactg gaaattacga taaaaacgaa gctaatgcta tggtaaatag
    18541 attagctaat atagatggaa agtatttaaa tgcattaaag caaaataact taaaaataaa
    18601 attattaagt ggaaagttaa cagatgaaaa agaatatgct tatttaaaag gtgtagttcc
    18661 taagggatgg gaaggtactg gaaaaacttg ggatgatgtt ccgggtttag gtggaagtac
    18721 agtagctctt agaataggat ttagtaacaa aggtaaaggg catgatgcaa taaatttaga
    18781 attacatgaa acagcacatg caatagacca tatagtatta aatgatatat ctaaatcagc
    18841 acaattcaaa caaatatttg ctaaagaagg tcgttcttta ggtaatgtaa actacttagg
    18901 tgtatatcca gaagaatttt ttgcagaatc atttgcatat tactatttaa atcaagatac
    18961 aaatagtaag ttaaaatcag cttgtccaca aacttatagc tttttgcaaa atttagctaa
    19021 atagagtttt aataaattta aaataaatat aaataaagag atgctattat aatagcatct
    19081 cttttatatt gcgtttaaat ctaataaatt ttgtcttatg tataactatg atagattatc
    19141 aattactact ccttatacaa ggcttcaacg tgtttattat gcatttgagt aattatcgta
    19201 ggtataatag aaattattga accaattact atcaataaaa ttcctataac catgtttata
    19261 gggataattt ctttaagtat taataatgct agtattggag atattactgg tttaaagaag
    19321 aatactaaag atgtaagact agtagatgta gcttccattg ccataaagta aagcgcataa
    19381 ccaacacctg taacaaatac gaatacataa atcataatgg gcatattgtg taaggtatat
    19441 ccactaaaaa tagggatatt tgcaaataca tgaagcttat ttttatttaa tatatctgcg
    19501 ataaaattaa ttttagtgag taaaaccaat atcaacattt ccaaactacc aaatataaag
    19561 ctaaagcagg ttacaactat tccaccaaat tttaaggttt ttcttttacc aaatacagta
    19621 tacattgaaa aaataatagc agaagataat gttaagaata ttccactaag agttagtttt
    19681 gtatgcaatg gatttattat aactataatt ccaagaactt ctaatataag tgaaattaca
    19741 ttgtgcctat atattttttc cttcaaaatt aagtaagcta aaagcattac aaagactgga
    19801 ttacaactga acaaaacagc aactacagat gcctttgtgt ttagaacagc aagttgatac
    19861 aatgacatac ttactactac acacataaaa cctaaaaatg caaagtattt taagtctgat
    19921 tttgtaatag aaagactaag agctcttaat ctttttacag caaaaggtag taagactaaa
    19981 ccgccaacaa aaaatcttga taaagttatt tgtaagggat taaagtcatt agtgacagtt
    20041 tttagactaa tttccattga actaaaaaat aaggtagtca gtattatata aacataacct
    20101 tttttcaagt caaacacctc cttgtaattt atattagtac tatagataag aatattcaag
    20161 catattggaa taatatggta tgtataaaat atagtacata aaattataaa ctaccttaat
    20221 atagaatgta agtattctat actaaggtag ttaattattt gaattacttt gatttagtta
    20281 ttttaaatta tataatttgg ctaatttttc aaatgctggt aaagaacttt taactttatc
    20341 ataagataca caatatgata ctctaaaatg acctggacaa ccaaaagaag aacctggtac
    20401 taataataaa ttaaattgtt ttgcatcatt gcagaatttt ttatcatctt caattggagc
    20461 ttttgggaac aaatagaatg taccattagg tttaacacaa gaatatccca tatcgattaa
    20521 actattatat agaaggtcaa aattcttttt ataaatattt acatcaactt ctgcatctaa
    20581 acttcttgca ataacccttt gaaaaagaga aggagcattt acaaatccaa gtattctatt
    20641 tgcaacattt aatgaagaca taacatcatc aaaatcatcc atttgcccat tagcaacaat
    20701 atatcctata cgttcaccag gtagagataa tgatttacta taagagtatc ctataaatga
    20761 atttctatag tattttagta cacaaggaac tttggcacca tcataaatta tttctctata
    20821 tggctcatca gaaattaggt atatgcttgt attaaattct ttttgttttg aatccaataa
    20881 ttctccaaga ttttttaata attcttcaga atatatgaca ccagtaggat tatttggagt
    20941 gtttattatt aaagctcttg tttttggagt aatagcattt tttagagcat ctaaatctgg
    21001 ctcaaaagtt tctatatttg taggaacttc tattaatttt ccatcatagt tttctgtata
    21061 atttttatac tcaccaaaat acggtgcaaa agcaataact tcatcaccag gatttaaaag
    21121 agtttttaaa ataatattta gaccaccagc agcaccacaa gtcataatta aattttctct
    21181 tgtaagattt aatccatctt ttttatttat gtgttcagca atagcatctc ttacatcctc
    21241 atatcctgag ttgttcatat atccatgaac taaatttgga gattcttcat ttaaaatatc
    21301 atttataact gctttaatag tctctggagg ttcaacactt ggatttccta tactaaaatc
    21361 aaaaacattt tcctcaccat aaatatcaga aagtttcttt ccttcttcaa acatagctct
    21421 tataacagaa ctatttgcaa ctaaagtttg cattttatta gatatcatat gtaacactcc
    21481 tttttataga tttatattgc ttatgtatat attaccatat ttttactgaa tttagacaat
    21541 tttcgaaaaa ttacttgtt
//
LOCUS       DAEMIK010000067        21331 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEMIK010000067 DAEMIK010000000
VERSION     DAEMIK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.067"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            306..1730
                     /locus_tag="KPK87_002058"
     CDS             306..1730
                     /locus_tag="KPK87_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG2538208.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1765..3894
                     /locus_tag="KPK87_002059"
     CDS             1765..3894
                     /locus_tag="KPK87_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2538209.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLIDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3924..4346
                     /locus_tag="KPK87_002060"
     CDS             3924..4346
                     /locus_tag="KPK87_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2538210.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4365..5516
                     /locus_tag="KPK87_002061"
     CDS             4365..5516
                     /locus_tag="KPK87_002061"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2538211.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5622..5723
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5906..6478
                     /locus_tag="KPK87_002062"
     CDS             5906..6478
                     /locus_tag="KPK87_002062"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG2538212.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6542..7192
                     /gene="fsa"
                     /locus_tag="KPK87_002063"
     CDS             6542..7192
                     /gene="fsa"
                     /locus_tag="KPK87_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG2538213.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7344..10268
                     /locus_tag="KPK87_002064"
     CDS             7344..10268
                     /locus_tag="KPK87_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2538214.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LR"
     gene            10857..11315
                     /locus_tag="KPK87_002065"
     CDS             10857..11315
                     /locus_tag="KPK87_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2538215.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            11376..11651
                     /locus_tag="KPK87_002066"
     CDS             11376..11651
                     /locus_tag="KPK87_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2538216.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            11710..12984
                     /locus_tag="KPK87_002067"
     CDS             11710..12984
                     /locus_tag="KPK87_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2538217.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPK
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            13114..14166
                     /locus_tag="KPK87_002068"
     CDS             13114..14166
                     /locus_tag="KPK87_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2538218.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            14166..15197
                     /locus_tag="KPK87_002069"
     CDS             14166..15197
                     /locus_tag="KPK87_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG2538219.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            15289..16101
                     /locus_tag="KPK87_002070"
     CDS             15289..16101
                     /locus_tag="KPK87_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG2538220.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            16104..17024
                     /locus_tag="KPK87_002071"
     CDS             16104..17024
                     /locus_tag="KPK87_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG2538221.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NVSTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            17199..17645
                     /gene="rpiB"
                     /locus_tag="KPK87_002072"
     CDS             17199..17645
                     /gene="rpiB"
                     /locus_tag="KPK87_002072"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG2538222.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERYARRLQMIDDIEKENL"
     gene            17675..18340
                     /locus_tag="KPK87_002073"
     CDS             17675..18340
                     /locus_tag="KPK87_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG2538223.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            18450..19952
                     /locus_tag="KPK87_002074"
     CDS             18450..19952
                     /locus_tag="KPK87_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG2538224.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(20264..>21331)
                     /locus_tag="KPK87_002075"
     CDS             complement(20264..>21331)
                     /locus_tag="KPK87_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2538225.1"
                     /translation="TYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFM
                     YIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVI
                     GDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDML
                     KNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQS
                     IVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDM
                     FIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENA
                     HMEIIIDADLFKVMLTFRRVN"
BASE COUNT         8648 a         2239 c         3929 g         6515 t
ORIGIN      
        1 acagataaat taaatctata ttagtatatc caaaagtaca tttaataaat ttatttaact
       61 gaattattat tttcatttct attaaattta aatattttat aaaaatagta ttatttacaa
      121 aatcatatac tccaaaatac tacataaata aaattaaaca aaaactaaaa tgacaaatgt
      181 cattatctaa agtgacatag aaacggtttc ttaaagttgt tactataaaa tataaactct
      241 ataataaaag tataacaaaa gggaatcata tcgatataga atataattca agaggaggta
      301 aaattatgag tagttctaat caatcgacaa taaagacatc tatacagcga gtagggaaat
      361 ttttaagtgg tatggtaatg ccaaatatag gagcttttat agcatggggt cttataacag
      421 cattatttat accaacagga tggatgccta atgaaaaatt gagtactata ggtgacccaa
      481 tgataaaata ccttttacca ctattgatag cttatactgg tggtaaagca atagcaggac
      541 aaagaggtgg cgtaataggt gcagcagcag ctatgggagt aattgttggt gcagatatac
      601 caatgtttat cggagcaatg ataatgggac catttgcagg atgggtaatt aagaagtttg
      661 ataaatttgt agatggaaag ataccaacag gatttgaaat gttagtaaat aacttttcaa
      721 taggtataat aggtatgctt cttgcaatac taggatttta tgcaattgga ccagcaatag
      781 tagcagggac agctcttata gaatctggag ttcaatttat agttagcaaa agtttattgc
      841 ctctagtttc agtatttata gaacctggaa aagtattatt cttaaacaat gcaataaatc
      901 atggtatatt agggcctata ggtatagctg aggcaaaaga agcaggtaaa tctataatgt
      961 tcttactaga aagtaaccct ggaccaggtt taggagttct attagcatat tggatgttta
     1021 gtagaggaag tgtaaaacaa tcagcaccag gagctgtaat aatacatttc tttggtggta
     1081 tccatgaaat ttacttccca tatattttaa tgaatccagt tttaatattg gctacaatgg
     1141 caggtggagc agcaggaata ttgacttttt caatattagg agctgggctt gtagcagcac
     1201 catcaccagg aagtatattt gcacttatgg cactagctcc aaagggagga ttacttccag
     1261 tattagcagg agttgcagtg gcaacagttg tatcattctt agtggcagca ccatttgtta
     1321 agagagcttc agcaaatcaa agtgaagaag acagtacttc tttagaagaa gctaaagcaa
     1381 aaatgagtga tatgaaatct gcaagtaaga actctgagaa aaatattgag gaaaaacagc
     1441 ttgaagtaaa tgagattaag aagattgtat ttgcatgtga tgcaggaatg ggttcgagtg
     1501 caatgggagc ttccagattt aaaaatagaa taaaaaactt agacttaaat atagaaatta
     1561 caaattcatc tgttgataat cttccagatg atacacaaat agtagtaact cacaatacat
     1621 tagttgaaag agttgctaag aataattcaa gtgttgagat agtttcaata aacaattttt
     1681 taaatgaccc aaatttagat gcattattta aaagattaga atctaaataa aaataaaaag
     1741 taccactaat taggagtgga taatttgaaa aaaaagaaga ttacatctag gcaaaaaaag
     1801 ataatactaa tgatagtaga aaattcaaag aaaaacattc caataactat ttctgagatt
     1861 gcagggactt tagagttaag ttcaagaact gtacttagag atatgtctgg aattgaaaaa
     1921 tggtttgatg aaaatgattt taactttgta aaaaaaccag gagtgggatt gattttagag
     1981 gaaaatatag aaaatcaaaa ctttataatt gagttgttag aagaagaaaa aatagaaaaa
     2041 gaatacagca aagaagaaag aaatttaatt atattatcaa agctactagt ttctaacgag
     2101 ccagtaaaat cctattattt tgcaaaaata ttaaaggtat ctgaaggggt actgaataat
     2161 gattttgcct tagcaagtaa atggttggaa agatttgata ttgagcttgt tagaaaacca
     2221 gggcttggtg tctatttaaa aggtcaagaa aaaagcttta gagaggcata tgtaaatctt
     2281 atatacgatt cattcaatga aaaagaaata ctagatatgg tcagaaatat aagtgaaaat
     2341 atacaaacag ataaagcaat agaaatttta agtgaaaata gacttttaaa tttaatagac
     2401 aggtgcataa taagaaaagt tgaagctaca ttaacaaaga agctttcaga tttagatgta
     2461 aatttagctg atagtgcata tatagggctt gtagttcata tttcattagc cttacagagg
     2521 ataaaaaatg gtgaaaatat aactatggat aaagagtttc taaaagaatt atctataaca
     2581 gaagagttta aattagctaa agaaatcgta aaaggtatgg aaacagattt taacatggat
     2641 ataccagtag atgaggttgg atatataact atgcatataa gaggtgcaaa gcaaaggtca
     2701 tcttcaaacc ataaggcttt aaacttagat gatatagaaa taatggaaat tacaaataag
     2761 atgatagatt tggcggaaga tgaatttaaa ataagtttaa aaaatgacga gaggttattt
     2821 aaagacctag caaatcactt agggccttct ataaatagat tgaatatggg attagaaata
     2881 agaaatcctc ttttagatga aataaaaagc aagtattcat atgcctatga tggagttgaa
     2941 aagatttcta gaattataaa agataaacta aatataaatt caatacctga atcagaaata
     3001 ggatatattg caatgcactt tgcatcagcc atagaaaaaa atcttatgat gaatactaat
     3061 ataaatattg ttgtggcatg tcccactgga ataggtactt caagattctt atcaactaag
     3121 attgaaaata aatttcctaa tttaaatata cttgaaacca tatcagctat aaacatagat
     3181 gaagagtatt taaaggaaaa agatgttgat ttaatagtgt ctacagttga gttaaataca
     3241 agtttaaatt atatatgtgt aggtccattt atgagtttag atgatgaaca gataatcaag
     3301 gaaaaaataa aatctatagc tcaaaataaa ttaatcaatt taaatgcgaa aaatgatact
     3361 aagagtaaga ataaagtata tgaacagata acagaaagta tgaatatagg aaaagatatt
     3421 cttcaatttt tagaggaaat tagatttgaa aaatttgaaa gtaaagattt aagtgagctt
     3481 attgaagact catcaaaaat atttgcaaaa tctagtgaag atataatctc tataaaagaa
     3541 tcgttaaaag aaagattaaa aatttcaatt ccatatatag aagaatcaaa aattctattg
     3601 ttacattgta tgagtgaaag aatagatatt atgaagttat caataataaa actagagaat
     3661 agaatcgtgc tagattcaaa tgaagaaatc gacaatgtag tttttatgtt acttcctaaa
     3721 aattcaccaa gttatcaaag acaaataatg agtgaaataa gtggttcatt aatagacaat
     3781 ataattttta ctaataagat aaataaattc tctatagaag agatggcatt agaggtaaaa
     3841 gatatagttt ttaattttta tacaaacaga ttaaaagcat ttatagataa ataaaattaa
     3901 tattaaaaag gaaggttgat actatgagta aagtattgaa tgagaataat atatttttag
     3961 ggttagatag tgtttcaaag gaagaagcta taactttagc aggaagaaaa ttagttgaaa
     4021 atggatatgt aaaagaagaa tatataccag caatgttaga aagagaaaaa gtaatgacta
     4081 catatatggg aatgggagtt gctattccac atggtgtaaa tgaagcaaaa aaagaaatat
     4141 tatcttctgg aatagttata ttacaattcc ctaatggtat agattttgat ggagaaaaag
     4201 catatttatt aataggaata gctggtgttg gagatgaaca tttagaaata ctatctaata
     4261 tagcagtagt tttagatgat gacttaacag aaagactaaa aaattcaaat gacaaacaag
     4321 cttttatgga agcttttgcc aactaatctt aggagggatt taaaatgaaa aaggcaattc
     4381 agtttggagc aggaaatatt ggaagaggtt ttattggagg cttgttagtt aagtcgggtt
     4441 atcatgtagt atttgctgat gtaaatgagg atatattaaa ctcaataaac aaagataaaa
     4501 agtatactat acacataaga gatgtagaat gtatagatga agtaattgat aatataagtg
     4561 cggtttcatc tataaaagaa gaaataatag atgagattgt acaggctgaa ataataacta
     4621 cagcagttgg acctttagtt ttgactaaaa tagcctctac aattgcaaaa ggtataaagg
     4681 ctagaaaaga aaagggatta acaagtaatc taaatataat agcatgtgaa aatgcaatat
     4741 acgcaagttc ttctttaaaa gaagaggtac ttaagtattt aaataaggaa gaagtagaat
     4801 atttagagat gtatgttggt tttccaaact gttcagttga tagaatagtt cctcctggta
     4861 aaaacgaaaa tcctcttgat gtaacagttg aaaattttta tgagtggaat gttgaaaaac
     4921 aaggatttaa aggggaaata ccaactatag ttggaatgaa tttagctgat aatcttatgg
     4981 cttatataga acgtaaattg tttacactga atacaggtca tgctataaca gcttatatag
     5041 gatatttaaa aggatataag actatagaag agagtataaa agataaattt atctgtgata
     5101 tagtcaaaag tgcaatggta gaaagtggag aaggattgat taaaaaatac aattttgact
     5161 cagaagtgca ctataaatat attgataaaa ttttaaatag atttaaaaat ccatatctta
     5221 atgatgatgt attaagagtc ggaagggaac cattaagaaa actaagtgat aaagatagat
     5281 tgattaaacc tttaatgaca gcaaaatcat atggattatc agttgataat ttgattcttg
     5341 gaataggtgc agcgcttcat tataataata gtgaagatac tcaaagtgta gaattacaag
     5401 agttgataaa gtctattggg gtaaaaaaag ccgttgctaa aatagctaat ataagcaatg
     5461 acgaagaact tttaaataat atcgaaaaaa gttatatatt tatgaaaaat ttataaattt
     5521 acttgaaaaa aagtagttct gataaagtaa ctataataga ttattctaca cagtttgaat
     5581 tatttgttga ataatagaga attataatat ataattaaaa ggaataaatt aatatattta
     5641 cctgtatatg tttgataata tggttcaaat gtttctacta agctaccatt aatagcttga
     5701 ctacaggagt gaagaagata tagtttatac ttattttaaa ttatatatta agttaattac
     5761 tttgaatttt aagctcctgt ctatgatata gacaggagct tttttagtat tttaatataa
     5821 aatacttaca agaatagata gcaaaattag aacaaatata atttttttat taatataatt
     5881 ttagaaaggg ctaggtggaa gttcaatgaa ggctttacaa gaaaagatat tgagagaagg
     5941 cagtgtttca ggaaatgata tactaaaagt tgatagtttt ttaaaccatc aaattgatgt
     6001 agctttttta aatgaaatag gaagagaatt taaagagaga tttaaaggag aaaaggtgga
     6061 taagatattt acaatagagg cttcaggaat agctatagca tcaattgttt cacagtattt
     6121 tgataatgca cctgtagtat ttgcaaaaaa atcagaatct aaaaatttgg ataaagatgt
     6181 atatgaaact aatgtatatt catttactaa ggcaagagag tattcagtta aagtttctaa
     6241 aaagtacata aacaaaggcg aaaatatatt gatagtggat gattttttgg caaatggaag
     6301 agctgctctt ggacttaaag atttaattga gcaagcagaa gctaacttag ttggagtagg
     6361 aattgtcatt gaaaaaggat ttcaagctgg aggagcactt ttaaaggcta atgatgtaag
     6421 attagagtct ttggcagtag ttgaatctat agataatgga actgtaaaat ttaggtaatt
     6481 tatgatatta tatacttgtg tttagcacat attttaacta tgtaattatg gaggattttt
     6541 tatgagattt tttatagaca cagccaatat tgaagaaata aaagaagcaa atgatttagg
     6601 tgtaatctgt ggtgtaacta ctaatccgtc tctaatagct aaagaaggta gagattttat
     6661 agaggtagtt aaagaaataa gtgaaatagt agatggacca ataagtgcag aagtaataag
     6721 tttagaacat aaaggtatga tagaggaagc agaaaaatta tctaagatac ataaaaatat
     6781 agttataaag atacctatga cagcagaagg tctaaaagcg gtgaaagtac tatcaagtaa
     6841 aggtataaag actaatgtaa cacttatatt ctctgctgga caagctcttt tagctgctag
     6901 agctggagct acatatgtaa gtccttttgt tggtagattg gatgatatat cacaaaatgg
     6961 tcttgattta attgaagaaa tcgttgatat atttagtgta aatagtattg aggctcaaat
     7021 aatagttgct agtgttagaa atccaataca tgttttacaa gctgctagaa tgggagcaga
     7081 tatagcaaca gttcctctta aagttataaa ccaaatgatt aagcatcctt tgacagacaa
     7141 aggaatagat agctttatga aggattggga aggtgcttct ttaaaattat aattataaac
     7201 tacgctttat aagtatatta aagaggttat tatggaaatt ctaagtaata aggctaggct
     7261 attttgccta gtcttatttt ggtgtataat atattgaaac atattattga aatagattaa
     7321 ttttttaagt gaggaggcga aaaatggata aaatattaag agagatacaa agagaagata
     7381 aaaaaaatcc atatactgac caagaacttg ctgaaatgtt aaatgtagct aggtctgaag
     7441 tgatatcagt taggaaaaaa aataatattt tagattcgag agaaagaaga aaaaaaatat
     7501 taataaaaga tatatcaaaa attgtaagtg aaaatcctca aatgtctgaa agaaaaataa
     7561 ctgaaatatt aatagaaaat ggatataata tatcgagaag tgcagtttca aaattattaa
     7621 aagaggaaaa tttaaatcag tcaaagattg ggattaagga taaagaccca gcatcatcag
     7681 gaaagtatgt gaaggtgagt aaagttgtag aagaagatgg atttgaagaa ttgataggta
     7741 taaagggaag tttgaaagaa aaggtaaatc tagctaagtc agcaataatg tatcctccta
     7801 atggccttca tactataata tatggagaaa caggagtagg taaaagtgaa ttagctacat
     7861 gtatgtataa atatgctata aaaaacaaca ttaaagaaga gaacagccca tttatagtgt
     7921 ttaactgtgc agactatgca gaaaatccta atctattgat agctcaactc tttggggtag
     7981 taaaaggtgc atatacagga gcagatgcaa atagagaagg tcttgtagaa caagctaata
     8041 atggaatatt gtttttagat gaaattcata gattgcctgc tgctgggcaa gaaatcttat
     8101 tttcattaat agatagagga gaatttagaa ggttaggaga aagtagttct aatagaaagg
     8161 caaatgttct cataataagt gctacaacag aaaatccaga ttctaattta cttcaaacat
     8221 ttagaagacg tataccaatg gtaataaatt tacctaatat gagtgaaaga ccaaaatctg
     8281 aaagatatga aataataata aagttttttg aaagagaagc aaagcgagta aacaagaatt
     8341 ttataataac taaagaagtt atgtgtgcac ttatgaatta taagtgtact ggaaatatag
     8401 gtcagttaaa gagtgatata caagtaactt gtgctagagc ttttagtaaa tcgatatttt
     8461 ctagcgataa agtaattata gatttagata gtttaaggga ttatataaaa agtggatatt
     8521 atgattgtaa ttgtaaaaat gaaaagtatt ctgagttttc agttgaggat atatatatag
     8581 atattagtga attggtgagt ggaaaaaata gaattttatc tgataatgag ataagcgaga
     8641 tatataatta tgcagaaaaa gagcttaagg ttttggaaag taagtgcttt tcagaagaag
     8701 agttaaaaaa tgcatttata gaaaagttag ataagaagtt taatgctatt aaaaacaata
     8761 aaaatttatc tgatagaaga agtcgaatag actggggagg acaattaaaa gagtctacac
     8821 tggagataat ggataaggta attgtattta ttaaggagca gtttaagcat gtaaatcaag
     8881 gtctgtactt ggctctagca attcatattg agcatgctat aggtaggata aaagatggaa
     8941 aaactataat aaatccgagt ttagataaaa taaaagcatc aatgccaatg gaatatgagt
     9001 tgtcaagata tataatgggt atagtagaag atttgacaca agtaacattt ccagaagatg
     9061 agcttggata tttggcttat tatataaata aattttgtta taatgaggaa agtataaaag
     9121 ataaagttaa agtagtaata gttacgcatg gtaaagttgg aattgaaatg tctaaagttg
     9181 taaatcatat tttaggtata gaatgtacac taggtataga aatagcactt acagattcac
     9241 catcagaggg gatagagcat gtattagaag agcttcaaaa gatagaagct agaaaaggta
     9301 ttctagttct aattgatatg ggttctttgg taattcttgg tgatgaggtt gaaaaaaggc
     9361 ttggaattag atgtaaaact gtaaatagag tagatacatt attggcaatg gaagcaggaa
     9421 aacttgcaac tattgaagga aaaagtttag atggtataat agctgattta aagaaaaata
     9481 agaattatgc tatgataaat acaaataaat ttagttacag aaaaaacgag tatggaaaga
     9541 aaaatgtaat cattacttta tgtttgagtg gtgtaggtac agctttaaat ttaaaagagc
     9601 atatagaaaa acaaattaag gaatatgaca ctttaattga agtaaagcca gttgcttttt
     9661 taaataataa tttagaagaa gagttaaatg atattgaagc tgaatataat attattgcta
     9721 tctgtggaac tattgatata gattataaaa acgtgccttt tatttcttat aatgaggtac
     9781 ttggtaaaaa tggtattaat aaagtattag agcatttaaa tagtaaacaa ccagctttaa
     9841 atattgaaaa ccagttgaat aatttaatac atgaagattt gattttatac aattttgaag
     9901 gaatttcaaa ggagtatata attgatactc ttgtttcaaa acttgaagaa ggtggatatg
     9961 tcgactcaaa gtatatatta agtgtatata aaagagaagc aatgggaagt gttgtaatgg
    10021 cctctaaggt agccgtgcca catggacttc cagaaaacgt tataaagcct gcaatagcaa
    10081 tcgcgaggtt gaataaacct atagtatggg acaataaatt tatggtggat ttggtggtac
    10141 tactggcact taaagaaaat aataagaaag aaatacgaag cttattttca aagattaatg
    10201 accagcatac tttagaagtt ttattaaata ctgaagataa aaatgaaata aaagaacttt
    10261 taagataatt gttagtaccg agaagaaaga caaaaaaatt tgctttagta taaaatttaa
    10321 aactactcaa ttgctaagta gttaattact ctatataagg ctagttctat gaaatttatt
    10381 tatatgattt tatagaatta gcttttttct tggtaaataa aatatatgaa tttatttatg
    10441 taaaaagtaa attttattgg acaattctat gagaattttt cctatatttc ttataaaata
    10501 tagaatatat aaaaaatata taaaaataaa ttaaattcat aataaataat tttataaaat
    10561 agttgttaaa cataaaaaac catttgtaaa aaacaaaaaa atcatttctc taacaaaaaa
    10621 aaccacaaaa aatataaaaa agtatggcta gttaagtgat taacaagtaa aaattaaaat
    10681 aaaaatcaaa aaaaatttat caatgaaagt cagctaaaat cctggaagag agcattattt
    10741 ttgggaaaac attttctttt gtttcacaaa aaaagccact ttttggcaca caacatgctt
    10801 tatataaagt caagaaaaac taaggtataa gacaaaggca attaggaggt gtaaatatgg
    10861 agtttataaa ttttgtaagt gataaagtta tagaccttaa aatggattgt aaaacgaaag
    10921 aagatttctt taaagaaata cataataaag tttttgattt aggctttgta aaagaagaat
    10981 ttggagaaaa aatattagca agagaaaatg tatttccaac aggtttaaat ttaggagatt
    11041 atggagtagc aataccacat acagatgcag aatatattaa agagcaattt atatcagtct
    11101 gtacttttaa tgaaccagta gtatttagtt ctatggaaga ccaagatgaa aaagtacctg
    11161 taaatttggc tttcgtatta ggacttaatc aaccacacag tcaattatct gtattaacag
    11221 aattaatggg aattatgcaa aataaagagt tagttgataa gttgatgagt tctactgata
    11281 aagaagaagt tttaaagaca ctaaagtctt tataaaaata aaaaagtact aaaaataaaa
    11341 aatataaaaa taaaaattat taggaggaaa taaaaatgaa aaaaatatta gtagcatgtg
    11401 gagcaggaat agcaacatca acagtagttt gtgacaaggt agagagatta gttaaggaaa
    11461 ataatataca agcagaagtt atacaatgca aaatagctga atgtgcaaca aagcaagaag
    11521 gagcagattt aatagtatca acaactatac taccaacaac ttataatata ccagcaataa
    11581 aagcaacagc atatataact ggagtaaata cagcagcatt agataaaaaa atattagatg
    11641 ctttaaaata atcctatcat agatacattg aaataatatt ttataaaaca gaaaaaggag
    11701 gatttataaa tggataaatt attagcagta gtacaataca ttttaaatat gggaccgaca
    11761 gtaatactac caataacaat tgtagtacta ggaatgatat ttagaattaa gtttacaaaa
    11821 gctttaaagt caggtcttac aattggtata ggatttgtgg gtataaactt agttgtaaac
    11881 ctattaacat caacactagg gccagcggct caaggaatgg ttgaaagatt cggacttaat
    11941 ttaacagtaa tagatgttgg ttggccaaca acagcatcag caacatgggc ttcaccagtt
    12001 gcaccaatat taataccagt ttgtttatta gtaaacttac tattattata cttcaataaa
    12061 acaaagatat taaacgttga tatatggaat tattggcact ttatagtagc aggtgcagtt
    12121 ggatatatag taacaggaag ttatgtttgg gctatattct gtgcaatact aatggagtta
    12181 ttagtactgt ttattgcaga aaagacagca aaaaatgttc aggagtttta tgatttagaa
    12241 ggtatagcat taccaactgg ttcaacagct tcatttgctc cacttggata tttagtaggt
    12301 aaatttgttg aaaaaatacc aggaataaat aaaatacaag cagacccaga ctctatacaa
    12361 aaaagatttg gtatatttgg agaaccaatg atgatgggga ttatactagg tataatactt
    12421 ggagcacttg cgggatatga tgtagcagga atattcaatg taggtatatc aatgggtgca
    12481 gtaatgttac ttatgcctag aatggttaga atattaatgg aaggtttaat tccaatttca
    12541 gaatcaataa gagatttctt acaagcaaaa tatggagaca gagaacttta tataggtctg
    12601 gatgcagcag tagcaactgg acatccagca gttatatcaa cagctcttat acttgttcct
    12661 ataactttgt tcttagcagt tatactacca ggaaacaaag ttttaccatt tggagactta
    12721 gcgacaatac cattttatat ggcatttata gtgtgcttta gaaaaggtaa tatagttcaa
    12781 tcagttataa caggaacaat agtaatagct atatcacttt atatggctac aaactttgca
    12841 gatgttcata cacaattatt acaacaagca aatatgatgc caaaaggaac aacaatggtt
    12901 acaagtatag atacaggtgg aaacttgcta aaatggggaa tattgaaatt atctcaatta
    12961 gtttcttcat ttggaatatt ctaatatgta gttaatagag attactcttt tttagtaaag
    13021 tatgcgaaaa tttaaaattt agcagctact aaaaaagagt aatatttttt ataaattttt
    13081 aaaaatacaa aatacaagag gaggagattt atcgtgaaag cagcagtatt acatggcaca
    13141 aatgatatga gatttgaaga tatagaaata aaaccatgtg aaagtgatga agtaaaaata
    13201 aaagttatgg cagcaggaat atgtgggtca gatcctccaa gagtattaaa acattggaaa
    13261 tatccagttc cagctatacc aggacatgag ttttctggag taattgctga agttggtaag
    13321 gatgtaaaaa atgtaaaagt tggagataga gtagttgcaa ttccttttat accttgtaat
    13381 gaatgtgaat attgtaaaag aggattattt tcattgtgtg atgaccatgg aatgttagga
    13441 gctaaaagtt ttggagcttt tgcagagtat gtaaatataa aagctacaaa tgtacttcca
    13501 ataggagata tggactttga agatgctgca atgatagagc cactagcagt tgctatgcat
    13561 ggagtactta atataggtgt tcaagttgga gacactgtag ccgtaatggg tagtggaaca
    13621 atggggcagt tggtaataca aggacttaaa atagcagggg caggaacaat tatagcagtt
    13681 gatatatctg ataataaatt aagagaatca aaggaattag gggctgatat aataattaat
    13741 gctaaagata ttaacccagt agaaaaaata aaagaactta ctggtggaaa aggtgttgac
    13801 atagctcttg aatgtgcagg ttcaaaaata actcaagaac aatgtttact tataactaaa
    13861 aagaaaagta agataggatt tttagggata gcatattcag atataacttt atcagaagaa
    13921 gcatttgaaa atatatttag aaaagaacta gaattaaaag gtttttggaa ctcttattct
    13981 gcaccatttc caggacaaga atggactaag ggaatcaatc tagttaatga aggaaagata
    14041 aaattaaaag aaatggtatc tcatagattc tcacttgaag atacttacaa agcatttgaa
    14101 atgataagag atagaaaaga agaatttaac aaaatattaa ttttaccaca aggggttgaa
    14161 aaataatgaa agcagtagtt aaaactaaac caggctatga taatgtagaa gttttagaag
    14221 tagaagaacc gaaagctaca ggagacaagg ttaaaataaa agtagaatat agtggtatat
    14281 gtggttcaga tatacattca tttaaaggtg aatatgcaaa tataaaagcc ccagtaacat
    14341 taggtcatga attttctgga attgttgtag aagtaggaga agatgttaaa aatataaaag
    14401 taggagatag agttacatca gagactactt ttgcaacttg tgagaaatgt atatattgtg
    14461 aaactaaaga ttataactta tgcccaacta gaaaaggtat tggtactcaa gtaaatggaa
    14521 gttttgctga gtatgtatta tcaagagaag aaagcataca tgtacttcct gaaaatgtat
    14581 ctttattatc agcagccctt acagagccac tagcttgttg tgttcatgca gctcttgaaa
    14641 agactactat agaaagttct gatacagttt taataatagg accaggacca attggtttat
    14701 tattagctca agttgttaaa tctcaaggtg ctacagttat aatgtctggt gttacaaaag
    14761 ataaggaaag attagaaata gctaaaaatc ttggagtaga tagaactgta aatgtaatgg
    14821 aagaaagttt ggcagatgta gtgaatgaaa tgactgatgg aaatggagta aacaaaggat
    14881 ttgattgttc tgggtttatg ccagcagtta acgaagcctt aagactgctt agaaaaaaag
    14941 gtactttcgt acaagttgga atctttgcta aaaaattaaa tgaaatggac caagaagcaa
    15001 taatccaaag agagttacaa tatataggaa gtagatctca aaagccaagt tcttggatta
    15061 cagcattaga tttaatggca agtgggaaag taaatacaga agctttagtt actagaactg
    15121 ttggtttaga cgaatttaaa gaaggtataa cagctttaat gaacggggaa gaaataaaag
    15181 tagcagtaaa atcttaaaaa aacatttgta ttattttaaa aaaaattgta aaatctactg
    15241 taggataaaa aaatgtaagg tcttaaaaaa taacaggagg aagaaagtat gagcactctt
    15301 agagatcatt ctaataatat tagaaaaaac ataataaaaa tggttgcaga agctaaaagc
    15361 ggacatccag gtggttcatt atcaatagct gatatgttga cagtattata ttttgataag
    15421 atgaacgtat gtgcagaaaa tccaaaaatg gctgatagag atagatttgt tctatcaaaa
    15481 ggtcatgcag caccagcttt atatgctaca ctagcagaaa aaggattttt cccagaagaa
    15541 gaattaataa cactaagaaa atttggttct aagttacaag gtcacccaga catgaaaaaa
    15601 gtagcaggaa tcgatatgtc tactggttct cttggtcaag ggctatctgc agcaaatggt
    15661 atggcacttg caggaaaatt agataacaaa gactatacag tatatactat attaggtgat
    15721 ggagaaatcc aagaaggtca aatatgggaa gctgcaatga cagcatcaca ttacaaatta
    15781 gataatttaa ttgcatttgt tgacttaaat ggattacaaa tagatggttc taatgaagaa
    15841 gtaatgaatg taaatccagt agatgacaaa ttcaaatctt ttggatggaa tgtaatagta
    15901 ataaatggac attcttttga agaaatagga aatgcagttg atgaagctaa aaaagttaca
    15961 ggaaaaccaa ctgtaataat agctaaaaca gtaaaaggta aaggtgtttc atttatggaa
    16021 aataatgctg catggcatgg aacagctcca aatgcagaac aagcagagca agcattgaaa
    16081 gatttagaag gaggtctata ggaatgggaa tagcaactag agaagcttac ggacaagtgt
    16141 taaaagaact tgcagaaaat aaagatatag tagtattaga tgcagattta ggaaaagcta
    16201 caaaaagtat atcatttaaa gaagttgccc cagatagatt ttttgacatg gggatagcag
    16261 aaggtgacat gattggtaca gcagcaggtc tagctacttg tggaaaaata ccttttgcga
    16321 gtacttttgc aatatttgca gcaggaagag gctatgagca aataagaaac tctgtagcat
    16381 atccaaattt aaatgtaaaa atagcagcta ctcatgcagg tgtaacagta ggtgaagatg
    16441 gtggtagcca tcaagctata gaggatatat ctctaatgag aggtatacca aatatggttg
    16501 tattaaatcc agcagatgct ttagaagcaa gacaagctat tcttgcatct attgattata
    16561 atggacctgt ttatataaga ttaggtagag ctgcaactcc tgatgtaaac agtgaaaatt
    16621 ataaatttga aataggaaaa ggaactgttt taagagaagg ttctgacata acagtaatag
    16681 ctacaggaat aatggtagca aaagccttag aagcagcaga agaacttgct aaagaaggta
    16741 taaatgtaga agttgttaat gtatctacaa taaaaccatt agatgaagca ttaataaaag
    16801 aaactgctaa gaaaactgga aaagtagtta cagcagagga gcacagtata ataggtggtt
    16861 taggttcagc tgtatgtgaa gctttagcag aaactaaaga tgtagtagtt agaagaattg
    16921 gtgttaaaga tgtatttggt caatcaggta caccagctga tttattaaaa cactatggtt
    16981 taactacaga agatatagta aaaaatataa aagagttgtt atagatataa atatatctaa
    17041 ataacaaatt gatttgacat aagtcaatta aagctgtctt aaaaatagaa agaaatttct
    17101 acaatgggac agctttattt ttacaatact ttatttttat gataattata aaatagtctg
    17161 aaagtaatca aaaaaattat aaaaagaggt gctatttaat gaaaatatct attggatgtg
    17221 accatggtgg ttatgaatta aaggaatata taaaggaaaa tttaataaat aaaggtatag
    17281 aagttgaaga tgtgggtaca aattcaaaag aaagtgtaga ttttccaata tatgcaaaaa
    17341 aggtaagtca aaatgttcaa agtaaaaaat ctgatttagg catactttgt tgtggaacag
    17401 gaataggtat gtctattgca gcaaataagt ttaaaggaat cagagcagct gttgtttctg
    17461 actgtttttc agctaaggca actagagaac acaataatac aaatgtactt tgtttaggag
    17521 aaagagttat tgggaaagga ttagcaatga gaattgttga agaatggtta aattcaagtt
    17581 ataatggaga aagatatgca agaagattac aaatgattga tgatatagaa aaagaaaatt
    17641 tataaatata taatttataa caagggggag cgttatgtct attatttgtg cttcaataat
    17701 gtgtgcagac caaatgaagt taaaagagga attagaagct cttgaagaag caggtgtaaa
    17761 attacttcat tgtgatgtta tggatggaat atttgttaaa aacttggcta tgggaccaga
    17821 gttattaaaa tcaataagtg aaaatacaac aataccttta gacatacact tagccacaga
    17881 aactccagat aaatatattg atatgatgtc ttatataaag ccaaaatata tttcttttca
    17941 tgtagaatca agtacaaatg taaaagctga tatacaaaag ttaagaaatt atggtatagg
    18001 gccagtgtta gcaataagcc ctcaaacaag tgtggataaa atagaagaat atataagttt
    18061 agttgagggt atcttagtaa tgacagtaaa tccagggttt gcaggtcaaa agtttaatct
    18121 ttctgtattg gataagttgg ataaacttac agagatacta aaagattatg ataatcctcc
    18181 atttatagag gttgatggaa atataaataa ggatactatt aaacttatga atggtaaaaa
    18241 agtagatata tatgttgttg gtacttcagc tttatttaat gataagcctc caatttcata
    18301 taaagataaa atagaagaac taaaagaaag tataaagtaa ccatgtaata aaattataat
    18361 atgtttctgt ttaatagtaa aaatttaggt ggaaacaaga aattttataa tatttaataa
    18421 aaattttggt tgagtaagga gaatttttta tgggagacct atataaacta caaaatggta
    18481 ctgatattag aggaatagcg tatgaaaatg attctaaaga agttaaccta actgtagaag
    18541 aagtaaaaaa aatagctaaa gcgtttcata tttggttaaa agaaaagact aagaaagata
    18601 aagtaactgt tgccatagga actgattcta ggataacagg aagccaattt agaagtactg
    18661 ttattgaaac tttaactaat gatgattgta atgtaattga ttgtgagatt gccacaactc
    18721 cagctatgtt tatgacaact attatggatg gatacaattc tgatggggcg attatgatta
    18781 cagcaagtca cttaccttat tactataatg gtttaaagtt tttcacagaa agtggaggtt
    18841 tagaaaaaac tgatataaaa gagatgttgg atatagctgt taaaaatgat agtgtagact
    18901 atgaggaagt aaataaaaaa ggtgaagtta tagtaaaaaa tttaatagaa gattattcta
    18961 atttattaat agataaaatt agaaaaggtg taaattcaag taaaaattat gaaaagcctt
    19021 tttctgggct taagatattg gttgatgcag gaaatggggc tggtggattc tttgctgaaa
    19081 aagttttaca catactggga gcagatacga caggtagtca gtttttgaac ccagatggta
    19141 tgttcccaaa tcatatacct aatcccgaaa acaaagaagc aatggaaagc atatgtaaag
    19201 ctgtgttaga taataagagc gacctaggaa taatctttga tacagatgta gacagagctg
    19261 ctattgtagg taaaaatggc aaaccaataa ataaaaatgc tcttatagct gttatatcat
    19321 caatagtatt agaagaacat ccaaaaacag ctatagtaac agattcgatt acttctgaag
    19381 gacttgctaa atttataaat gaattacaag gaagacatca tagatttaaa agaggatata
    19441 aaaatgtaat aaatgaagct ataagattaa atagtgaagg tgaagagtgc catttagcta
    19501 tagaaacgtc tggacatgct gctttaaaag aaaattattt cttagatgat ggggcatatt
    19561 taatagctaa aattttaata aaagtagcaa agctatcctt agaaggaaag actatagaag
    19621 aattgataga aaaccttgaa gaagctcaag aagagaaaga agttagaata ggcattaata
    19681 aagaagattt tagaccatat gcagaggata tattaaaaga tttagattct catgtcaaga
    19741 gtattgatgg atggtctgta gcaccaaata attttgaagg cataagagtt aattgtgatg
    19801 aatcaaatgg agatggatgg tttttactta gaatatcact acatgaacca ttattagctt
    19861 taaatattga atcaaataaa attggcggag cagaacaaat ttattctaag ttaaaacttt
    19921 tcttagaaaa atatgattta aaaggtcttt agggtaaata gaaagaatat atttatattg
    19981 aagaataaat gtaaatttgt acctaagatt acaaatatta caaaattgta atcttaggta
    20041 acagagatgt aactgattat ggaaacacta tgtgatatta tagatacaga gagatgaaat
    20101 atgtgatgaa ttatagagaa atataattta aaatgtatta atatcacacc aaaaaggtat
    20161 aatcaaaaaa tatgtaaata taaaccccaa catttagaaa aagacctcta aggaggtttt
    20221 tttcttgttt aaataactta aaatatattt ttatatcaat atatcaatta actcttctaa
    20281 aagtaagcat taccttaaat aaatcagcat ctataattat ttccatgtgt gcattttcta
    20341 attcaattat attttgagca attgataaac caagaccaga gccatctgtt tttctagata
    20401 tatcacccct tctaaatctt tctattaact cttctggctt tagatttagt ttatcatttg
    20461 aaatattttt aaacgttaat attatatttt cttcctttat aaacatatct atatagaccc
    20521 tagtgttagg catagagtat tttaatatat tgcaaattag attctcaaaa actctccaag
    20581 tttttttacc atctatcatg gtaaaaacag catattcagg tgtttctatt ataaaatcta
    20641 aactgctatt ttgaattttg tcttcaaatt ctacaatact ttgaattaaa agttctctta
    20701 tatcggtttt tgttatatta aattctacat ctccactact tagcttagat gcttcaaata
    20761 tgtcatctat taataatttt aatctttgtg attttttatc taaaatattt atatagtcat
    20821 ttatagtctc tctgtcaaag tcattatttt ttaacatatc aacatagctt aatatagatg
    20881 taagtggagt ttttaagtca tgagatacgt ttgtgataag ctctgccttc atcctttcat
    20941 tttttatatt agcatctaca gcagtttcaa gaccgcctgt tatattattt atacttttgg
    21001 ctaaagtaga tatttcatca tctcctataa cttctaattt atactttaaa ttgccatttt
    21061 ctatattttt tatatttttt gatatgttat ttatgtaatt aaacttaaca gataaaaagt
    21121 tgtatatata aaaatgaaaa actgtgttta caagtgtaaa taaaattact atagctattg
    21181 cctcagcata atatatatac ataaagaata tcaaaacaag atattggata atagcttgta
    21241 caactataga tattaaaaat actatagcta attttttatt tatagttttt ttattataca
    21301 tactaagtaa gtttgaaaag aatacatatg t
//
LOCUS       DAEMIK010000068        21140 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEMIK010000068 DAEMIK010000000
VERSION     DAEMIK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.068"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(131..508)
                     /locus_tag="KPK87_002076"
     CDS             complement(131..508)
                     /locus_tag="KPK87_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2538226.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(695..1666)
                     /locus_tag="KPK87_002077"
     CDS             complement(695..1666)
                     /locus_tag="KPK87_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2538227.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(1700..1897)
                     /locus_tag="KPK87_002078"
     CDS             complement(1700..1897)
                     /locus_tag="KPK87_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG2538228.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(1965..3176)
                     /locus_tag="KPK87_002079"
     CDS             complement(1965..3176)
                     /locus_tag="KPK87_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG2538229.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            3457..4059
                     /locus_tag="KPK87_002080"
     CDS             3457..4059
                     /locus_tag="KPK87_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2538230.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            complement(4315..5286)
                     /locus_tag="KPK87_002081"
     CDS             complement(4315..5286)
                     /locus_tag="KPK87_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG2538231.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEANVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(5312..6793)
                     /locus_tag="KPK87_002082"
     CDS             complement(5312..6793)
                     /locus_tag="KPK87_002082"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG2538232.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKIKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDKSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            complement(7206..7631)
                     /locus_tag="KPK87_002083"
     CDS             complement(7206..7631)
                     /locus_tag="KPK87_002083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2538233.1"
                     /translation="MSEETENAFRRVLKRKQKPTLKDIDGYRNFLFISPNGNPMQESN
                     YKSVLNGVVKKYNKNHEKTPLLKITPHSLRHTFCTQLVQKNMNPKNLQYTMGHASITI
                     TLDLYAYASEAGANRELRKHDSMIYNDLYNYCEQKYKGL"
     gene            complement(7788..8045)
                     /locus_tag="KPK87_002084"
                     /pseudo
     CDS             complement(7788..8045)
                     /locus_tag="KPK87_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790768.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8389..9240)
                     /locus_tag="KPK87_002085"
     CDS             complement(8389..9240)
                     /locus_tag="KPK87_002085"
                     /inference="COORDINATES: protein motif:HMM:NF012703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HBG2538234.1"
                     /translation="MKTIRLLYPDYVSGGLEQYFFGANLMSHILPENENQPIVKVEIT
                     PPNRKEKLVTDGIYGKNEVIAGIKNATEKIEKENPDKIITIGGNCLVSQAPFDYLHGK
                     YNDLGIIWIDAHPDVSTVNDDYPNAHAMVLGSLMGYGDNALSSLMKNKKFKADEILYV
                     GLQEIHDYQKKFLDETCVNYKIQTDEFLSDKEILSFIERFENIVVHFDIDVLDENLFH
                     STYFANPDLVGDGSGGGKMTLDKLGNILHCITENSNVVGFTIAEYLPFDEYKLHKLFS
                     KIRMFTE"
     gene            complement(9660..10646)
                     /locus_tag="KPK87_002086"
     CDS             complement(9660..10646)
                     /locus_tag="KPK87_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2538235.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            complement(11031..12785)
                     /locus_tag="KPK87_002087"
     CDS             complement(11031..12785)
                     /locus_tag="KPK87_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG2538236.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(13003..13893)
                     /locus_tag="KPK87_002088"
     CDS             complement(13003..13893)
                     /locus_tag="KPK87_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2538237.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            14042..14902
                     /locus_tag="KPK87_002089"
     CDS             14042..14902
                     /locus_tag="KPK87_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2538238.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            15358..15924
                     /locus_tag="KPK87_002090"
     CDS             15358..15924
                     /locus_tag="KPK87_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2538239.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            15964..16545
                     /locus_tag="KPK87_002091"
     CDS             15964..16545
                     /locus_tag="KPK87_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2538240.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(16602..17519)
                     /locus_tag="KPK87_002092"
     CDS             complement(16602..17519)
                     /locus_tag="KPK87_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2538241.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIEVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            17649..19280
                     /locus_tag="KPK87_002093"
     CDS             17649..19280
                     /locus_tag="KPK87_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2538242.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            19283..20230
                     /locus_tag="KPK87_002094"
     CDS             19283..20230
                     /locus_tag="KPK87_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG2538243.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(20515..20778)
                     /locus_tag="KPK87_002095"
     CDS             complement(20515..20778)
                     /locus_tag="KPK87_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG2538244.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(20877..21122)
                     /locus_tag="KPK87_002096"
     CDS             complement(20877..21122)
                     /locus_tag="KPK87_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538245.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         7005 a         3323 c         2720 g         8092 t
ORIGIN      
        1 tttaggcatt ttactaatta gagaacctat ttaatattac tttatgaata gtgagtagct
       61 gattaaataa taccatttat ggttttcagc catagattaa ttaaattctt agagttctat
      121 cctgatataa ttactcttct tctaaatttt taaaatgact ttcaaataaa tccaacatat
      181 ctttatcaac aacttcatca tcatgtagtg ctgaaattaa actctgtaaa gaattgtcgt
      241 gtatattgct taaaaaatct tttgtctcaa cacttaaata ctccttatgt ccaacaacta
      301 ttgtataatg agtgtatcta tctatttttt ctgcacttaa aaaatgtttt ttaacaagtc
      361 ttgataaaag tgtcaatgta gttgtttgtt tccaaccata ttttcgttcc atagcttcaa
      421 tagtttcttt tgatgttact gtatcatttt tttcccaaat aaacttcata acttttaatt
      481 ctgcttgtgg tatttttgaa attgtcataa ccttacgccc ctctcaaact tttattaaat
      541 taatactatc gcaatatagt ttttaagtca aagattttgt cgataataat attttccaat
      601 ttttaccata gggttatttt ttatataatt ctatgataaa ttacataaaa aatatttatt
      661 ttacatcata aaattagaaa tgtaaatagt atgattattt agtcttatct ttaataaaca
      721 atccagtaaa tttatacatc caaagtgcat tcataaacaa caataaactt gttgaaaaaa
      781 atactgttct aatacctaaa tgtgctgata tttgtccccc taaaaatgct cctgatgaac
      841 agcctatata ctgggcagat gcattatatc caaatatttt tcccacatat tcttctggtg
      901 caattttttt aagtaagctg ttaacagaag gattaagccc tgcaactgac aaccctaata
      961 aaaatctcaa caacatcagt tgccatgcag tttttacaaa agcttgtggt ataaatataa
     1021 tcccagccca taaaagtgaa attaacaata ctttttgagg acctatttta tccgacattt
     1081 ttccaagttt ttgagctgct aataaatttg ctaatcctga tgcagaaaat gtcaatccag
     1141 ctattaaagc tacatttgat gcaaaatttg taagttgtga tatatatatg gttacaattg
     1201 gttctataga gtataaagct agttgagtaa tgaatgtggt tacaaaaata gatgcaatta
     1261 aaaatttatt tggcaatata tttaattgtt ctttaggtat agaaattgat tcttttattt
     1321 ctttaggttt aaaattatct actacaaata aaatagagat gataaatgat acaaacaaca
     1381 atcctcctgt tataataaat gtacttttaa gacctaagat tgattctata tatcctccta
     1441 ctgttggacc aattaatgag ccagtagttg atgcagttgc aagagtaccc aaagcccatc
     1501 ctgaatgatt tttatctgtc tgtgttgcaa taagagtagt acatgcagta gcataacccg
     1561 taaaaactcc ttgaagtatt cttaatccta ataactgata gacattttga acaaacgcca
     1621 tggtaaatat aactattccc atcccaatac ttgcacgtaa aagcattgat tttcttccat
     1681 atttatcagc agctttccct caaattggcg aaaagaatgc tgctactaca aaggtacatc
     1741 caaaaattat acctgaatac tgttgtatta aactaacatc agttaccccc aactgtttaa
     1801 tatataatgg aagtataggt gctatttggc tcatccctat agaactaaaa aacataccta
     1861 tccaacatac aaataaattt cttttccaca attccatata gaattacacc ccctgcattt
     1921 taaatttttt caataatact aagtttatta atttatgcta ttgtttactc aatatatcca
     1981 tcttttcttt tataatattg tttaggaaat tcataacttg aaaataatag cggtgataaa
     2041 gaataggagt aacttccagc attactaatt gaaatcaaat ctcctatgtt tgcttttttc
     2101 aatttaacat tatttgcaat tacatctaat gatgtacaga gattcccaac aacatttaca
     2161 tactcttctt cttttaaatt atttaatact gaaagagaaa aggcatgatt tgatgtatat
     2221 aatggctctt gaccttctaa ttctactcca caagtaatat tttcaattaa attagaaata
     2281 ataggtctca taaatccatt aagtccattt tttacaataa gatatttttt ccctcttgat
     2341 actttaatat ctacaatttt aacataataa ttacctgatt tacaaactat gaaacgtcct
     2401 gattcaataa gaagtcttga ttttagtgtg tttgaattag taaaagcaat cttttgagtt
     2461 aattttgaaa gctctttaag attaagtgga ttttctctaa gattatcata agctactcca
     2521 agtccactac caaaatttat aaattcaata ttaatatact tacttttatt taaatttaaa
     2581 gctataaaaa aacatttttc ataataatgt cctaactttt caaaatccaa tacctgagat
     2641 ttaatatgta tatgaatccc aactacagat acataatcac acatacttaa catcttaaaa
     2701 acactgctca tatcttctat atctatacca aatttgcttg atttagcact gtcatcatcc
     2761 atagtaaaat caggatttat tcttattcct attttaactt tctgattatg ctttttagat
     2821 atcttattta taagttctat ttctgtaata ctatctgcaa taattatgca tttatctaaa
     2881 acagtttcta tatcaatctc tgttttgcct ggtgatgaat aataaatatc atctttagaa
     2941 actccacatt ctgatgaaat cataacttca ttagttgaag aagcatctgc tccaaaacct
     3001 tgctctgcaa tgctttttac aattttgtta tatggatttg tttttataga atataaaaaa
     3061 tcaaaaccta aaatattatt tttcaattcc ttacattttt caattatatt agtctcatca
     3121 tatatataac atggtgcatt ttcttctata aatttatagt cttcatttga gtacatattt
     3181 tttacctcca agattttaaa tataatcata aattaagcaa ttataatcct ataagataat
     3241 tttacaataa attaccatag ttaactacac acatatatgt aaaaacatac ctaaaatatt
     3301 tttaattata gatacattcc aacttttatt gataaaacaa tttgtttcaa ttttattaca
     3361 aatatacaaa gtgtattgtt gttaacgtat tctcatagta tcctaaaact atattaagca
     3421 ctaaaaacta ttttaaaata ctgtggaggt atcaatatgg aagtatgcat tcttatggga
     3481 agtcctaaga aaaaaggtaa tactgctgct attttaaaac cttttattga ggaattagaa
     3541 aattataatt caaatattga agtaatatgg ttatatgatt gtaaaataga gccatgtatt
     3601 gcttgtaaaa agtgtcagga taacttattt gattttggat gttattgtaa agatgatgtt
     3661 cagaaaattt tcaagaaaat tcttatgtgt gatttaattg tttttgctac tccaatttat
     3721 tcttggtact gcacttctcc aatgaaagca ctacttgaca gattggtgta tggaatgaat
     3781 aaatactatg gtaatgagaa aggccccgct ctatgggcag gaaaaccagt agcacttatt
     3841 acaacatgtg gatatagacc tgaaaagggt gctgatattt gggaaaatgg tatgaagcgc
     3901 tactgtaagc attcacagtt aaaatatata ggaatgcttg ttgaacgtga tttaggtaaa
     3961 cctaatttta tgaataaaga aaaagaagaa cattctcgtc aatttgcaaa acaaatttat
     4021 tatattttat ctaattcaat tgaattagat aactgctaaa gacataatac tccaagtaaa
     4081 gtattgataa tataaataaa tcttacataa tttgcttatt taaaataaga tatatattct
     4141 tctaaaaagt aacaataatt aaaatatttt ttatcatttc aacaatgata aagaggctta
     4201 tctctatttt gagacagcct ccttattgct ttttatatgt taattatttt catagtaaac
     4261 atccttaaaa actctttaca tgttacttac ttcaaaacct attactcatt cctactataa
     4321 attttttata aattcagtta tttcttcctt tgttgcatat gaacttatcg cacctttaat
     4381 agttgtacta cgagctgcat aataattaga aaattctaaa tactcattta acgtttcttg
     4441 tttcatttct tttatatcat tcaatgttat ttgtttatac aataaggtgt ataaaaatga
     4501 acctatataa gaatctcctg ctccggttgt atctattgca tttactttaa cccctttaca
     4561 catacctttt acttttttag tgtatgcatc tactccatct tttccttttg tatataatac
     4621 tagttcaaca ttccccacaa agagtttatg taatgatttt tctatatcat tttctcctgt
     4681 tataaattct aattcttcat cagatatctt aacaatatga gcaaaaggta aaaattctga
     4741 tattgccttt ttacaagatt gctcactatc ccataaaggt aatcttatat tagggtcaaa
     4801 gcttattata ctattacttt caagtgcgta ttctattgct tttttatgtg ctaatttcat
     4861 tggactatct attaaatcta ctgaacaaaa atgtaggata tgacaattat taaaccactc
     4921 ttttttaact tcatcagctt ctaaaagcat atctgcactt ggatttctat aaaatgagaa
     4981 atcacgatta ccatcttctt ttaatgatac aaaagcaagt cctgtatttg ctttacttgt
     5041 tctcaataca taatcagtat ttacatttgc ttcatttaat acatctatta tataatcacc
     5101 aaatgcatct tttcctaact tagatataaa acttgatttt cctccaagct ttgctacaac
     5161 agcactaaca tttgctggag ctcctcctgc tactcttcta aattctgata cattttttaa
     5221 tttaccacca ttttgattcg gtataaagtc tattaaagct tctcctatgc taattacttt
     5281 tttcatcttt tctacccctt ataatgtgtt tttataattc aataccttgc tttaatttaa
     5341 atttagtata ctcatactct actattttat cactataaac ccttatttga ttagaatttt
     5401 ctttagcaaa tattctacta gtaaatacta cttctccatc atttataaaa atttctaaaa
     5461 tacttctatc cgctagtact ttgatattag tattttcatc aatatttata tattcttttc
     5521 tatttgttcc atattcaaca ccgaaaactt ttttaaagtt gcttttgtct aaagttacaa
     5581 tgtttcctaa tttgttaaat tctaatttta caccttcatc ttgtgatgaa aataattcca
     5641 gcccaaattt attgctgtca atatttttta tattaatatt aagctcatat acattttcat
     5701 tattttctat aatattgata ttattcttta tagtattttg cccacttgtt tcatcatatc
     5761 ttaatttaat taattcatct acaggcattt gcaatagctt attatttttt atatttaatt
     5821 ttcttggaac agttagacaa tgtgcccatt tgtttttatc tgtagggtat aaaacttctc
     5881 ccattccagc ccatgcaaat agtattattt gtgagtcctt tataaatgtt tgtggagcat
     5941 agaaatcaaa tcctttttcc agttctttca ttgtatctat ttcaaatgtt aaattatcta
     6001 aatccatatt accaatagca tatactacat tatatatatt ttgataatca aatcctttag
     6061 gttctactcc ttgtggtgaa aatattaata tatctttttc atctatatta atataatctg
     6121 gacattccca catgtaacca aaatcttcat ctatattttt tacatttaat tctcctttaa
     6181 aattccaatc tatagagttt ttagattcat atactattat aactcctttt ttatcacttg
     6241 tttgtgctcc aagtaacata aagtatttat cctttatttt aaatactttt gggtctctta
     6301 catgtcctgt atatcctttt ggtatttcac ttactattgg attattttca tatttagtta
     6361 ttttaccatc cttgttcatt atagctaagt tttgatatgc atatctatct tctgcactat
     6421 attttatatt ccctgtataa tacatatgca attttccatc tacctctatc gcattacctg
     6481 aatatgctcc atgagattca taactttcga ttggtattaa tgctacatct tctctattcc
     6541 agtttacaaa atcatcagaa cttacataag cccaatgctt cattccatga gttgcatcat
     6601 atggatacca ttgataaaat acatgatact taccattata atatgctaat ccatttgggt
     6661 cattaagtag tccatgttgt ggatgtatat gaaatagtgg tttatattta tcatttaaag
     6721 atatatttct taatttattt aattcttctt ttgtcgattc taatatggtt ctatatttag
     6781 gctctttgta catattttta ttccttttct tttataattt tatatttgct ttattattgc
     6841 tatctaaaac aattcttagt cttattacac aatgtgctac accttttata ttttatttat
     6901 ttcctatgta ctctaataat tactttgata ctttgtaaca atcagttatt tttgcactca
     6961 ttttgctatt ctatcggttt aattaaaccg gtttcgaaac cggtttaatt aaagattaac
     7021 ataaatttat ttcagatgca atatttcttt caaaaaagtt ttcctaattt ttagaattat
     7081 agttttttag taaaaaacag catagatttt ttagtatttt tttataacta tcacgaaccg
     7141 tcaatatatc cctgtttata agggtctagc gtttttttca tgttgcaggt atactgtgga
     7201 actggtcata atcccttata tttctgctcg caatagttgt acaaatcgtt gtaaatcatg
     7261 ctatcatgct ttcgcaattc tctgtttgct cctgcctctg acgcataggc gtataagtcc
     7321 agcgttatcg ttatgcttgc atgccccatt gtatattgta aatttttcgg gttcatgttc
     7381 ttctgtacaa gctgtgtaca gaacgtatgt cttaggctgt ggggtgttat cttcagcaac
     7441 ggtgttttct catggttctt gttgtacttc tttacaacac cgttcagcac cgatttataa
     7501 ttgctttctt gcatagggtt tccgttggga ctgatgaata ggaaattgcg gtagccatct
     7561 atatctttga gcgttggctt ctgcttcctt ttcagcactc ggcgaaaggc attctctgtt
     7621 tcttcgctca taggcacgtt gcggaaactg ctctttgtct ttggctatgc tatgtagaaa
     7681 ccgtccttat ctttaagtag ctgatgattg atacggatta catggttctt gaaatccaaa
     7741 tcattctttg tcagtccgca aagctccgag atacgcaagc ctgttttcct tgcctgtaag
     7801 acgtgttcca agtgccgaca gcccataccc ctcaattccg taaaacgccc atagaaacgt
     7861 ctttagaagc tgtaacgata atctctccat agatatattg ttcttcatag ccaaacagtc
     7921 cgtaatcttc atgaataatt taagttttgc ctgctgatgt gcagttcctt tggcagtctt
     7981 gtttgggaat aaccgcaggc gttccgcttt tctttcaatg cttcaataaa ttcaatgtca
     8041 ttcatgtttt attcctccaa tcaaaaaagg catacgccca taggagcata caccttgtat
     8101 catgtatttt cttgctgtcg atatactgtt ttccaccaat tgacaaggtg cgaagtacag
     8161 taagtgctta taaccttata aatcgttgca tatcgggatt tttgtaattg ttctgtctct
     8221 tgtttcttgt aatagtcccc ccctgttaga taacggggct tataaggctc gcttcgcttg
     8281 ccaagccgcc gaagtgaacc acccctagcc agttgcgata tgtcttacga ccgccacagt
     8341 gggcggacca ctccgtcggt ttgaaatatt ccacgttaat caaataagct attcggtaaa
     8401 catacgaatt ttagaaaata acttatgtag tttgtattca tcaaaaggca gatattctgc
     8461 tattgtaaat cctacaacat ttgaattttc tgtaatgcaa tgaagaatat ttcctaattt
     8521 atccaatgtc atttttcccc cgcctgaacc atcgccgacc aaatcagggt ttgcaaaata
     8581 ggtggaatgg aaaagatttt catcaagtac atcaatatca aaatgaacga ctatattttc
     8641 aaatctttct ataaacgaca aaatctcttt atcagataaa aattcatctg tttgtatctt
     8701 gtaattgaca catgtttcat ctaaaaattt tttttgataa tcatgtattt cttgaagtcc
     8761 aacatataat atttcgtctg ccttaaattt cttgtttttc ataagacttg acaacgcatt
     8821 gtccccatat cccattaaag aaccaagtac catagcatgt gcgtttggat agtcgtcatt
     8881 gactgttgaa acatcagggt gtgcatctat ccaaataatt cctaaatcat tgtattttcc
     8941 atgcaaatag tcaaatggag cttgtgaaac caaacaattt ccaccaatcg tgattatttt
     9001 atctgggttt tctttttcaa tcttttctgt ggcatttttt attcctgcta taacttcatt
     9061 ttttccataa ataccgtctg tcactaattt ttcttttctg tttggtggtg tgatttccac
     9121 tttaacaatg ggttgattct cattttctgg caaaatatga gacattaaat ttgcgccaaa
     9181 aaaatattgt tctaaaccac cactcacata atcaggatat aataatcgta ttgttttcat
     9241 ggtatcctcc taaaattata attttcttca attatagcat gatatatttt tatcgtcatt
     9301 tcttttctgc ttttgaaaag ttacttacca gtccatgatt tgtaaaggat gctattcaac
     9361 attgcttacg caataagcca ttgacaaacc atagactaat aagttttaag gtaatcaagc
     9421 agaaatgaaa tagtatgctg ttccactgca tagaaaaaga cactctgttc aagtgccgtt
     9481 ttcttatatc tttgtatttc tctttataag tcgttacata aggctctttc atttgttcta
     9541 attttccgtg ttttcttttg gttgttttgt tgatagatac tgtatttatg tggatatttt
     9601 cgattttcta tctgttttta ttaaaaaaat acacattact aatatatgta atgtgtatct
     9661 taatttttat ttattgtact ttcaccttct attaattcaa atttaatctc ctttaatagt
     9721 ggttcttttc tttcttgtat taagtttact atcgtatttg ctgctacttt acctgcattt
     9781 ttattttcaa actttattgt tgtaagttta gggcttataa tagacaatac atcatatccc
     9841 ccaaatccag ctacagatat atcttgtggt atacttttac ctctcttatt gatttctttc
     9901 attgttgcta ttgctatatt atcagttgca catattatta tatctggatt ttcactttct
     9961 agcatttctc cacttctttg tatagacgtt tcttggtcaa agtcagtata aaatactttt
    10021 gcatcatacc ctttatcttt taacccatta agtactccat tttttctatt taaaccaact
    10081 gatatatcac tttcatcaac acctaaatat acaatgtttt tgtaaccttt attagcaata
    10141 tattgaccca ttttatatcc tgctccataa tcatcattca ctatgctatt gataccatca
    10201 actttttgtc caactacaac tatcggtata tctaactttt caatttcatt cttatgttct
    10261 ttagttattt cggtaccaat tagtattatt ccatcaactt ttagactagc taactttgat
    10321 atactatcta tttctgacct tatttttcta ttagtattaa taattagtga tgtatatttt
    10381 aattctttga gttcttcatc tattgccatc ataattttag attttacaaa agaatctaaa
    10441 cacggtgtta taataccaat aaagtatgtt tttttagctc tcaaactttg agcaaatgca
    10501 ttcggctcat atccatattc atctattatt gtctttattt tctctttagt agaatcactg
    10561 atatctttat tatttagata tcttgataca gtacttttag atactcctgc caagttagca
    10621 atatcattta ttgttatttt tttcataggt tatcaccttc tacatttgat atattttatt
    10681 acattatatt atatatttaa aataaaatat agttatttgt ccagtcaata ctttttatat
    10741 actacttgta ctatccatat ccacatccaa agaattgagt ctttaacatt aaattaattg
    10801 taattttcat tttttgttgt tatatttaat ttcaatttta tctactatga attgataaat
    10861 actttaattt atttataata taaaaataag gctatgattt ttacaaccat agccttatcg
    10921 ataaatactt aattatcact taaaaaataa ttttttattt aaagaatata tataaatatt
    10981 ttaaaattct ataattttta aaactatata aactttattt tcatatgttt ttattctcca
    11041 attgatatat tatttatgcc attattttta acttcaataa ttggttgtcc atttcctaaa
    11101 atataatctt tagaccaacc ttctggcaat ttatttatcc catttccaat taacttcaag
    11161 tgtaaatttg atggttcttt tttcagattt aatttaaata ttccatcaaa ggtaccttta
    11221 ttatcgcttc caaataaact tatattgcca tttatattcg tagctgttac atttacaatt
    11281 ccattgtttg ctctaaattt tatttcagat gatatagttt cagctgttac acctgaaatc
    11341 ccactattag tatttatatt gaaaggattt gttacattgt tatcaacaat gttagcaatc
    11401 ccgtcattga catcaacagt agctcctcct ttaatattat aaagttctac tgaaccactt
    11461 tcaacatcta ctttaaaatt agaatacatt ttatcaggaa ctccaatttt tatcacattt
    11521 accatcctat ctttatttgc atttatcata ttgtcgccat tcatatcttg caaatcacta
    11581 tctttcttat ctattttttt cttagcactt acaactagct ttccattttt tatattatta
    11641 gaagaatttt ctttatatac agaaaaagta caattttttt cattagttac acttacgtaa
    11701 tcaaactcaa atttattggt attcgtcttt tcaacaacca cacctaaatt atctgattct
    11761 ataagtattt cagaaacttc atttccttca actgaagtta agtgttttaa aatatttgga
    11821 gtcatatcca tattattata tttaatcata tctaaacact gtttatatat attggcatca
    11881 atacctccaa cttttgttat acttttagct ccttttaaaa tacctttatt acttccatca
    11941 gcaactaaaa cgattggttt ttctttggct aaaggagata atgttagtgc atctactaat
    12001 tgatatccat ttgttatata aaaatctgta gctccattat aaaacttttc cattaccttt
    12061 ttattggtct caaatctatc atttcctccg attctaattg ctttagtttc atttactaaa
    12121 tcctcattta tcgcagattt gccacctata gcgtaaacat ttaaaccttc agtcttaaat
    12181 ggaactgatt tcccatctgt aagtattata gctcctttat tttttgcaac tacaggtgct
    12241 atactcatag catcagcctc tcctacaaat ccatttgtaa gcattacctc ttttacacta
    12301 cttacttcat ttattttttt agctacatta taactagttt caattctatt gttaccattt
    12361 attctttcca cttgaatacc ttttcttttt atatcttttt ctactgaatt ttcaatagta
    12421 ttataaccac caattatata tacttttttt acaacattta aactcttatt atctagtcta
    12481 tttaaagtag cttttggtat ttcacttttt ttagtcaaaa gtattggtgc gttaatagct
    12541 ccagctaatc cacttgatga aagcccatca gataaatttt tatctccatt aactaatatt
    12601 acagtatcat aagtttgtct atctgctata agtcctgctg tttcatatct atcaattcct
    12661 attattgaat cttctttaat ctgtgcattt gcagttaaat tattaaaaag aaacatagtc
    12721 aaaactaaaa ttaatatact tagtttttta ctgaattttt tgaatttttt attcataata
    12781 tacactattc ctcctgtaaa ttaaggtctt taatttgctt ataaattatc cccttattta
    12841 caaataataa cataatatta tagtaacgta tatattacct taaaattatc agtaacttta
    12901 tatcaatcat cttagcttat ttatataaat ttttttatat tacttttcaa ataacagtca
    12961 tactaaaaca atttcatcat ataatttgaa aattattact atttacacag cttttatcat
    13021 catcttacca ccatcaaata tatattcaac catattattc tcatataaat aagttatata
    13081 agaacgtatt gtacttccaa ccaacacata ttgattagca tcaagcttga cattgtacaa
    13141 atcacacatt ttttcaataa cttcatcaat cataactggc tcataacaaa cctttttaat
    13201 attatctatt atttcttcca tcttcttctt attaatatta atcaaacttt ttatatctgt
    13261 agttttgaca gagtgacttg gaacaaagaa atcagcctct aacattctca atttttctag
    13321 tgtcttaaac tgtgaatcta tatccagtag gaagaaaaaa tgatatttag taattatatt
    13381 ctctggtgta agactatctg caacaaacaa aacattatct ggagttctaa caccaatcat
    13441 ttcaaaataa tgacctggaa gagaaatcgc ttcaagttct gtatctacaa ttttgccatt
    13501 tgaaggtata atataatcaa cttcactttc ctttgctaat aaaaatttat tcttcagtgc
    13561 tttacttgga tagccaccat ataaaaatgc tgattctaat attggatttt ctgtaaaata
    13621 tccttcaatt ttagtcgtag caatcttgca ttttgtttga ttcttcaaat atgcatttcc
    13681 tcctatatgg tctgcatttg aatgagtgtt tattattaat ttaggattta aattatgttc
    13741 ttctaataat cttaaaactt gtctagctgt atccttatta tttccactgt caattaaaat
    13801 tgcatcctta ccatctacgt atacacctac tattgtaggg agtggaatat aatatgtatt
    13861 tccttttata tgttctagag ttaatttagc cattttataa cctcccttta aatttacttt
    13921 taattatagc attaattttc tgaaaattcc aatcaaattg tttttataat atatttttat
    13981 ttcttttatt aaaaattgga aaaactatat aatatatatt ataatctata tataaggagt
    14041 gatgttattg aaaaaagata gaataacttt ttcagaaatt ttaatattaa ttgtaggctg
    14101 tatattaatg gccatttctc ttaacttatt ttttaatcca catgcaatcg ctgctggtgg
    14161 tataacagga cttggagttg ttttaaattc attattcggt gtagaacttt ggattgtcaa
    14221 tttactttta aatgttccat tattcatatt tgcttacaaa atactttcaa aaaaagattg
    14281 ttttaaaacg gttttaggaa taatttttct taccatagca cttaagctta ctgcaaatat
    14341 ggctacatta gatataacaa atgatatgta tcttgcaatt atatctggtt ctattttaat
    14401 gggagttggt caaggactta tttttagaat aaatggctct acaggtggaa ctgatttaat
    14461 ggcactccta ctaaataaat attttcctac atttagtata cctgttttaa tgggtattgt
    14521 agactgcgtt gtagttgttt tatcaggtat agtaaataga caggttgaaa ttgcacttta
    14581 ttcaactgtg gctttataca ttttagtaaa agttagtgac ttgcttattg aaggatttaa
    14641 ttattccaaa tcattcacta ttatatctga cctttcaaaa gatataagta aaaaaataat
    14701 ggaagattta gatagaggtg caactattct aaaaggagaa ggtgcatata ctggagaaaa
    14761 taaaaatgta cttttagtag tagttgaaaa gaaagaagtt gtcgaattga aaaaattagt
    14821 aaaaaatgtc gaccctaatg cttttattat aattacagat atacatgaag ctttaggtaa
    14881 tggatttaaa aaaatagaat aactaacaaa taaatagcaa gtataattaa catatacaaa
    14941 ttcaactttt tatatgttat attatacttg ctatttatat attatacttt ctatttatat
    15001 attataattg ttgtttttaa gataaaaatt aaatgtatca attattgttt tatacaagta
    15061 tttccaaatc cgttctcatt tagatagtat acttagaaat tttagaatta ttgagtctaa
    15121 ctttattact tacttcactt tataacaatc aaatacaaat ttaaatatta taaaactaca
    15181 aaataattta aagcatataa tgtcaatatg tttaaattat aaaatattca tgtagttata
    15241 taataaataa tttataaata tagccaactt agcctaaata cgtatataat atagctaata
    15301 tggttcttat acttgatacc atcaataaaa ctaaaaatat aaaaaggaga acttaaaatg
    15361 agtgttagtt tacaaaaagg acaaagagtt agcttagtaa aagataataa tcctgtaaaa
    15421 aatctagtag taggcttagg atgggatatg aataaattgg gtaaaaaaaa ttatgaccta
    15481 gatgcatttg caatagcatt gactaaccaa gataaaatga atgttagagg tgacttggtt
    15541 tattttggaa atcttaaaca cccttctaaa gctattatac acactggaga taacttaact
    15601 ggaaaaggtg aaggtgatga tgaacaaata attgttaatt tagaagacat acctgaatat
    15661 gtacataaaa tagtttttgg agtttctata tacaaagcta aaaaaagaga ccaggatttt
    15721 ggtcaaatga acaactcttt tataagatta attgattcaa attccaagca agaactattt
    15781 aaatataatc ttcaagaaga cttttctggt aaagtaacag ttttggcagg agaaatttat
    15841 cgtagaaatg aagaatggaa atttaatgct ttaggaattg gtcaaaatga agagcttcgt
    15901 gaattaatta atacatataa gtaataatta caataaaaaa tttataatga ggagttggtt
    15961 taaatgggta taactttagc aaaaggacaa aaagtaagtt taacaaaaag taaccctggt
    16021 ctaaaaaaag taatagtagg actaggatgg gatattaata aatatgatgg tggctttgat
    16081 ttcgatttag atgcctctgc atttttaact ggaactgatg gaaaagtaac gaatgatgga
    16141 gactttatat tctataataa tttaaaacac gcttctggag ctgttgaata tatgggtgat
    16201 aatagaacag gtgttggtga tggtgatgat gaacaaataa atgtagactt atctaaaata
    16261 cctcaaaata ttgctaaaat atcatttagt gttactataa atgaagcaat tacaagaaga
    16321 caaaatttcg gacaagttga aaattcatat ataagaatat ataatgaaga aactaatgag
    16381 gaacttatta aatatgaatt gggtgaagat tttagcatag aaacagcaat agttgtggca
    16441 gaactatata gacataatgg agagtggaaa tttaatgctt taggttctgg atttgaggat
    16501 ggacttgcag gattgtgtaa aaattttggc gttaatatag gctaatatat ctaaataaaa
    16561 ataagagtat attgatatac tcttattttt atttagatat attataaact gtttacactt
    16621 ctactcttct ttctcgcttg ttttttccca tattctactg aaatatcaaa aaatgctgaa
    16681 atattttctg taaaaaactt gcttctatga tatgcaatat caaatgtcct atgtaaatta
    16741 ataccttcaa tatcacaagc ccacaaaaga tgctttttta aataatcctc aataagtaat
    16801 tcagatataa ctgcaatgcc aaaattatct acaactgctc ttaatatagc ttcagagtta
    16861 taacatgacc atctaatatt cattgggatt tttaattctt ttaattgctt ttctaattgt
    16921 gctcttgtac cacttcccaa ttcacgcata attaatggtt gatttgaaag ttctgaaact
    16981 tctatagagt ctcttttata aaactcatgc ttgtgtgaac atataagtac aagattatca
    17041 ttaataatgc tttttgtaac aatatctggg tgcttaattt ttccttctac aatcgcaata
    17101 tctaattcac tattaagtaa tttttttgca atcagtcttg tatcttctac aaatacttca
    17161 ggttctataa gaggatttac cttgtataat tctaacatta tcgggcttat tacacatgta
    17221 ccaacagtta ctgttgcccc aactcttata cggatatttt ttgcacaatg cttcatacaa
    17281 tcattcattt cttcatttac agctaaaaga tgcctcccat atccaagaag ttttttccct
    17341 gcctcagtta aatataattt ttttgaaatt ctatcaaata gcttaacacc atactctttt
    17401 tctatttctg aaatagcttg gctaactgat gactgggtaa tatacatatt tcttgctact
    17461 tcactcattt taccacattc agcaacctta acaaagattt caagactacg aattgtcata
    17521 ttatcacaac cttagcaaac attagtaaat gcttatgatt ttattaatat tttagtattt
    17581 tacaaatata ttgtcaagaa gtataatttt attatagagt tataaagtag attggagtgt
    17641 gatatattgt gaaagtatta attataggtg gagttgctgc tggaacaaaa actgctgcaa
    17701 aaataaaacg tgaaatgggt gataattgct ctgttacaat actaaataaa ggtgatgata
    17761 tttcttatgc aggttgtggt cttccatatt acgttggtaa gataattgaa gataaatcat
    17821 ctttaatcgt aaatacacct gaaagttttt ctaaactaac aggagcagaa gttcgttgtg
    17881 gagttgaagt tacgtctatt gatagaaatg caaaacaagt aactttgaaa acgctttcaa
    17941 caggagaaaa agaaaatcta agctatgaca aacttgtaat tgctacaggg gcagacccaa
    18001 taaaaccacc aatacctgga atagatttac ctggagtatt ctttatgcgt actccaaatg
    18061 atgctataaa actaagagat tctattgaaa acggtattaa gagagctgtt gttattggtg
    18121 gaggtttcat tggtcttgaa attgctgaaa atctttctgc aatgggtata cgtgtaagtg
    18181 ttgttgatat ggctgaacat gttatgcctg gatttgatac tgattttgct gaatacgttg
    18241 aaaatcatat ggctgataaa ggcataatga tttttacagg tgaccaagtt gtaggtattg
    18301 aaggtgaaaa taaagttgaa aaacttcgta ctaaaaatag agctattaaa acagatttag
    18361 tggttatgtc agttggaatc cgtcctaaca caggttttct aaaagacaca ggtttagaat
    18421 ttgcccctaa taatacaatt attgcagatg aatatatgct tacaaatgac aaagatatat
    18481 acgtagttgg agactgtgct tttgtaaaaa attctttaac aaataaacct acatggtcac
    18541 ctatggggtc atctgcaaat catgagggta gaatttgtgc acaaaatata tctggaaaat
    18601 ctaaaactta caatggtgtt cttggaacaa cagtagtaaa attacctgag ctcaatgcag
    18661 gaaaaacagg tctaagtaaa gaaatagctg aaaaagaagg ttataatgtt tgctctgtaa
    18721 ccatagctac tgatgataaa gctcattatt atccaggtgc atctaatttt attataagaa
    18781 tggttgctga aaaaaatact agaaaattat taggtgttca agttatggga cctggagcgg
    18841 ttgataaaat agtagatatc gctgctactg caataacttt aggcgctgac ctttattcat
    18901 tagaaagtat ggaccttgca tatgcaccac ctttttcaac agctattcac ccttttgtag
    18961 tggcagtaaa tgttttacaa aataaactta atggagaact tgatagtgtt ttacttgacg
    19021 aaattgagga ttttgacagt tggactaaac ttgatgtttc aaaagctcct tctattccta
    19081 gtttacgtta tattcctgtt ggtgaaatta atggagaaat tgaaggcctt gctaaggatg
    19141 aaaaaattct tcttatatgt gctaaaggta aaaatagcta tatggcgcaa aatcgcttaa
    19201 gacgctttgg ctatacaaat acgaaagttc ttgaaggtgg aatactattt tatccaaatc
    19261 ttaaggtaat ggaggagtaa atatgagtaa tgttacagca gaacaaaaaa aggaagtaaa
    19321 aggtagaggt tttctaccaa gcagggatgg agaacatttt gcagcacgta taataactgt
    19381 aaatggtgta ataaatgcat cacaagcaaa aaaaattgca gaagctgctg aaaaattcgg
    19441 aaacggtcag ttagcattta ctactagact aacagtagaa ttacctggtg taaaatttga
    19501 agatattgaa gctctaagtg aatttattgc tagcgaaaat ctaataacag gtggaactgg
    19561 atcaagggtt cgtcctgttg tggcttgtaa aggtactgtt tgtgttcatg gtctagctga
    19621 tacacaggca cttgctgctg aaatacacga agaattttac aaaaattggt atgatgtaaa
    19681 actacctcat aagttcaaaa ttggtgttgg tggatgccct aataactgta ttaaacctga
    19741 cctaaacgat cttggaattg ttggtcaacg tgtacctgac tatgattcag agctttgtgt
    19801 tggttgtaaa aaatgtgctg ttgtagaggt ctgtcctgtt aaagcagcta aattaacaga
    19861 taaaggtaaa cttgaaatag atagtaatct atgtaataac tgtggtaagt gtatagaaag
    19921 ttgtaatttt gatagtattg aagaaaaaga atctggttat aaagtctata taggagggaa
    19981 atggggaaaa tcagttcgtc ctggaacaca gattgataga ttattttcaa aagaggaact
    20041 tatgacttta attgaaaaag caattctttt atttagagaa cagggtaaca caggtgaacg
    20101 ttttggaata actatcgata gaatcggtgc tgataaattt atagaaatgc ttttatcaga
    20161 tgaagttttg gaaagaaaag aagagatact agaggctctt ttacacctta caggtggagc
    20221 tgtatgctaa caataaatat tttaattgta cagataacct atggaaaaaa ttttagttaa
    20281 gaataattaa aattctatat atgatatatg ataaatgagg ctatctcaaa acacagatac
    20341 gcctcttttt cattgttgaa atgataaagt atatttaagt ttatacaaaa aagagtgtct
    20401 cgtgaattat aaaattcatt ttgaggcacc cacattattt tataataatt gcttattaat
    20461 tttattttaa ctatataaaa agtacaaaaa gtaagaatat ccaatattct aaaattaatt
    20521 ttgaattgta ttgattgaaa agtttactat tccactattt ccatcttgat ttactactag
    20581 ataccatgtt ccagtattag gaactctcat tctacatgga tttttctttg caaatccacc
    20641 atagtaatca taatttcttt gatttttaaa tttcacataa tttatatggt ctaacaatat
    20701 tacattggct tgtttctcta aatttacctc tacaatatcc ccttttttta agttcatctt
    20761 actataacta tattccattg aattactcct ccagtatcga ataatctttg ttatttactc
    20821 atctcaaata tgtaaaaatt catttaaaat attacaaata ctatataatt agactattat
    20881 ccagttttaa ccttatttac tacaatccaa aggttattaa aaatattatt ggcaacaact
    20941 tctccatccc atacaactct ttcttcttta ttttctaata aagcatagta cccagagtca
    21001 gaactttcat ctttatagac tgtaacataa aactcttcat cactatattc aggtatattt
    21061 ttaaagtctt cagatatttt acatgttaat atataacctt ctaattcttc atctataaac
    21121 aaagcaattc ctccaatctc
//
LOCUS       DAEMIK010000069        21107 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEMIK010000069 DAEMIK010000000
VERSION     DAEMIK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.069"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            689..1720
                     /locus_tag="KPK87_002097"
     CDS             689..1720
                     /locus_tag="KPK87_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2538246.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKCSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1853..3151
                     /locus_tag="KPK87_002098"
     CDS             1853..3151
                     /locus_tag="KPK87_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG2538247.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3265..3510)
                     /locus_tag="KPK87_002099"
     CDS             complement(3265..3510)
                     /locus_tag="KPK87_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG2538248.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3658..4524
                     /locus_tag="KPK87_002100"
     CDS             3658..4524
                     /locus_tag="KPK87_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG2538249.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4528..5064)
                     /locus_tag="KPK87_002101"
     CDS             complement(4528..5064)
                     /locus_tag="KPK87_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG2538250.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5255..5458)
                     /locus_tag="KPK87_002102"
     CDS             complement(5255..5458)
                     /locus_tag="KPK87_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG2538251.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5749..6513
                     /locus_tag="KPK87_002103"
     CDS             5749..6513
                     /locus_tag="KPK87_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2538252.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6902..8215
                     /gene="prdC"
                     /locus_tag="KPK87_002104"
     CDS             6902..8215
                     /gene="prdC"
                     /locus_tag="KPK87_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG2538253.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8619..8704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9286..11469
                     /locus_tag="KPK87_002105"
     CDS             9286..11469
                     /locus_tag="KPK87_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG2538254.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKPSDSQTPDVEKPSDGQMPDGEKPLDEQ
                     TPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQ
                     VFTSKDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVG
                     ASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSIL
                     YAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERLKQYQGSINLCD
                     KLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGKPSLSNGLTKIN
                     FEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTDEVEISTQQKFKLKS
                     VGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNG
                     QEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPP
                     VPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNPPVPPVSDTVEK
                     TPELSRDDTIVKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            11876..13636
                     /gene="prdR"
                     /locus_tag="KPK87_002106"
     CDS             11876..13636
                     /gene="prdR"
                     /locus_tag="KPK87_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG2538255.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            14065..15945
                     /gene="prdA"
                     /locus_tag="KPK87_002107"
     CDS             14065..15945
                     /gene="prdA"
                     /locus_tag="KPK87_002107"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG2538256.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            16026..16310
                     /locus_tag="KPK87_002108"
     CDS             16026..16310
                     /locus_tag="KPK87_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538257.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            16348..17073
                     /gene="prdB"
                     /locus_tag="KPK87_002109"
     CDS             16348..17073
                     /gene="prdB"
                     /locus_tag="KPK87_002109"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16798..16800,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG2538258.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            17153..17911
                     /gene="prdD"
                     /locus_tag="KPK87_002110"
     CDS             17153..17911
                     /gene="prdD"
                     /locus_tag="KPK87_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG2538259.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17926..18393
                     /locus_tag="KPK87_002111"
     CDS             17926..18393
                     /locus_tag="KPK87_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG2538260.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            18411..18893
                     /locus_tag="KPK87_002112"
     CDS             18411..18893
                     /locus_tag="KPK87_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG2538261.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            18913..19920
                     /locus_tag="KPK87_002113"
     CDS             18913..19920
                     /locus_tag="KPK87_002113"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG2538262.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            20040..20921
                     /locus_tag="KPK87_002114"
     CDS             20040..20921
                     /locus_tag="KPK87_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG2538263.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8408 a         2441 c         3875 g         6383 t
ORIGIN      
        1 tacaatataa atattaaagc gacatcttat agtttattga acctatagta atgaattaat
       61 aaatagttta attctatgaa aagatttcct tttatgcaat ttatttattg aaatatgata
      121 taatatataa aggtgtaagt attagttgtt aaaataatat ttatattagt ttttatgcat
      181 acttggtata agtttttcat atattgatta aaattgatgt tgatttatta tttttagtat
      241 tttttcacct tttgattcat actttgtaga atggtgtatt ttctaaaact gattataaga
      301 caagttcatt caaattagca tttttatatc atataaataa ttgttccccc taaataagat
      361 ttagatgtat agtattaaaa tcaaaaagta taaaataaaa ttgatgctta atcatagata
      421 aatattaata aaatatacaa ggggattgtc taataaaaag ataatctcct tttttacaaa
      481 taattctaaa ttgtgatata aatctaaaaa ttttatctta acatttaacc ttttaatgat
      541 aataattgat ataataaata taaattaaat atatggtgta cttattttat gtttttagca
      601 agaaatatag catttaaaag ataaattgtg caacttaaat caaagtttaa gatgaactat
      661 taacatagat atattaaaaa cttttgtaat ggagaatata tgtgatatga ataataaagt
      721 taaggttgca gtattagata caggaataga caaagaacat gattacttaa aagataattt
      781 agttggagga attgcttttg aatgtatgca tgattatatc tttatatcag ataaatttga
      841 tgatgaagat ggtcatggaa ctgcttgtgc atcaataata aagaaagagt atgaagatgt
      901 agagttattt gtaataaaaa ttttgagaaa agatggtatt acaaatataa aagttcttga
      961 agaagcatta agataccttt tagacactaa cataagatta attaatttaa gtttatcagt
     1021 cataggagta gaaagtgtta agggcttatt tgaagtttgt tatgaattgt ttagaaaagg
     1081 aaaaataata gtatgttcac tcgctaatga ttttgactta agttatccag ctatgtttaa
     1141 caatgttata ggtgtaagag gatttacttt agatggagat gattcatttt ggtacaacaa
     1201 aaaatatgat gttcaatgtg ttatggatag caattcatat ataagttgtg atataaataa
     1261 ttcctataga ttaccaccaa aatgtaatag ttatttatca gcaaagctta ctggtaaaat
     1321 agctaagata ttatcagaaa aaccaggcat aacaatatct gatttaaatg ataagcttga
     1381 atcattagca actagaaatc attgggatag ttgtgatttt gatgagtgta gtagaattgt
     1441 aaactttaaa ttggatttgt atgacaaaga aaatgctctt tttatgaaag ttgctgacgt
     1501 tgttagggat tgcttagata ttgaagagaa taatgaaaaa ctctttaagt gtagcctttt
     1561 taataaggag ataggcctgg tttatgataa ttgttttaat ctgttgaaaa agctagagca
     1621 tagatttgac ataaagttta attatatgga catctcaaaa tatgacttag tttcaatata
     1681 tacactgact gaattagtgg aaagatataa aaacttataa ataaagattt gtagctaaat
     1741 ttaatgtaga aggtattaaa ttatatgtat tctttgtcag tagattaaaa gtaaaaaata
     1801 tactataatt agatagcata agagaaaatg ccaatacagg gggagttaga taatgaaata
     1861 tgaagaagca ttagagtaca tatcacaaac taataaattc ggtataagat taggattaga
     1921 aaatatcgga aagttattag aattattagg aaatccacaa gaaactttga atataataca
     1981 tgtagctgga actaatggaa aaggttctgt gtgttctttt gtttctaata tactaagaga
     2041 gtgtggatat aaagttggtt tatatacatc tccatattta gaaactttta ctgagcgtat
     2101 aagagtaaat ggacaaaata taccacagga tgatgttgct agaataattg agttaataaa
     2161 agaaaaaata gagattatgg taaaagaagg ctatgcatat ccaacagaat ttgaagtggt
     2221 tactgcaatg gcattttatt actattcaga acaaaaggta gactttgtgg cattagaagt
     2281 tggtttaggt ggcagatatg atgctacaaa tataataaca aaatcacttg taagtgtgat
     2341 agtatctata agtttggacc atacaggtat acttggagat acaatagaaa aaatagcgta
     2401 cgaaaaggct ggaataataa aggaaaatgg agtagtacta gtatatgacc agactgatga
     2461 agcaaaggat gttataaagt cagtttgtaa agagaaaaaa gctaaatata tagaagttga
     2521 ttttgatgat ataaacataa aaaaatcaga tataaattct caaatatacg attgtactgt
     2581 aatgaaagaa acttatagag accttgaaat aaaattgata ggcgaacatc aaataaacaa
     2641 ttctatcttg gcaattagtg taataaaata tctaaaagac ataaataaat tagcaaatat
     2701 aaatgaagaa tctataagaa aaggacttat aaatactaaa tggccaggaa gaatagaaaa
     2761 gataaaggaa aatccaattt ttataataga tggtgcacat aatgaagatg gagcaaaatc
     2821 tttggcaaaa gcacttgata agaattttaa aggtagaaaa ttaaccttat taataggtat
     2881 gttagaagat aaagatattg atagtgtttt agagatattg ttgccacatt ttaataaggt
     2941 aataacaaca acaccaaata acccaagggc cataaattca gatatattaa gagaaaaagt
     3001 attaaaatat gtagatgatg taacatcaaa gcatgagata gaggatgcag taaattatac
     3061 attagaaaca tcaagtgagg atgatattat aataagtgca ggttcattat atatgattgg
     3121 tacagtgaga actttagtaa agaaattata attttataaa ataaatatac aaataaaaat
     3181 ataaaaaaga gttgtctaaa aaatggacaa ctctttttat ataaaataag tttttaataa
     3241 ttttactgat gtttatataa gtaattatat agattgacgt atagctttag ataattggtc
     3301 tgggcaagaa gtaccttttc caccacagtc aataccattt aatttatcag caacttcaag
     3361 agcattttgg ccaattatta agcttctaag tcctgataaa ttaccattac atcctcctat
     3421 aaactcagca tcaacaatca cattattttc atcaacttca aaaatcattt ctctacaaca
     3481 tacaccactt ggattaaatg ttactttcat taaaaagacc tccattaaat attatttctt
     3541 tttttaacat taataataat accaaatata tgcgtaataa tgcaactata aagcataata
     3601 caagctaatg aaataatata ttattgtagt atatgtattg tagagaggag gggttatatg
     3661 aaaatctata tttctaacta cttatcaaag agtattagca tagttgatta ttctacactt
     3721 gaactagaaa aagaaatagt attagaagat aatatatatc cacaccattt ttgcatagaa
     3781 aaagaaaaaa atttaatata catacctagt tcaagtaatg ggatactcta tgtcttagat
     3841 ttaagtaatg ataaaataat tgatactgtc tctataggag gaagtcttag tcaagttgta
     3901 cttagcgata atgaattgtt tgtggcaaat gaagattcta atagtatata tgtactagat
     3961 aaaaacacat taaatccaat aggaattatt ggagttgata atatgccaca tggatttgac
     4021 tttgatagag aaagcaaaaa gttatatgta ccgtgtataa actcgatagt atgtatagat
     4081 acaattaata aaaatataca aaagaaaata aatatggact ttagagcttg gcatatagaa
     4141 cttgataaac aaaagaaaga aatgtatata tcaacacttg atggaaagct tataataata
     4201 gatgaagtaa gcatggatat aaaaaaggta ttatatgaat ttttattacc agtagaaata
     4261 agatttaatt atagtggcaa aaaagtttat gttgctgatt tgggttataa taatgtaaga
     4321 attttagatt atacaacgag tagatacatt ggaaatattg aaataaatgg gattccccaa
     4381 ggattagaaa tatctaaaga tgaaaagtta ctatttgtat cagatacaca agaaaattca
     4441 gtaaaagttt atgaaactgc aaataacaaa ttaataaaag aaatcaaggt aggaaaagag
     4501 cctacaacta taatttgttt gtaggcttta atgtatgtct cttataagta aatcgataac
     4561 ttcggcatac atataagatg cattattttt ccagttattg caaataagtt ttgcttgttt
     4621 ctctgatgaa acatttagat ttaaatctat taagttagac tgattttcta tgactctaag
     4681 gttgacaata aattcattat cactttgttt tacaaaacta gactttactt gagtatctgc
     4741 tagtagattt tctttgttag aatctatata cttgtctatt ttttcagtaa cactagtagg
     4801 tattcgatta aagaagtatt ctagactttc tacgcctctt tgagtaagag cataatattc
     4861 tctgtcagag tctttatatg tagtcaaaaa cttagattcc ataagctgag ataaaagctg
     4921 ttgtaatgaa aaataattca tcatctcagt ctctaatact acttgagtta tttgagaatt
     4981 agttaaatcc attttaattt tttctaatat atataaaatt aaaagtttgt gataggctaa
     5041 ttcttcagac gagttttcaa acataaatat catcctccgt caaactattt ctatatataa
     5101 tatattaaac taatttgtac aaaattaaaa gaacaatttg tattgtatta ttaatcttta
     5161 ataattcata taaaaacaat tttataaata aatatagaaa aacagggctg ctaattagca
     5221 gccctgttga tacccaggtg tcaaaattta ctatttattt tccagccatt tgtctttcag
     5281 ccatttcaac taatttctta gtcatatatc caccaacata tccattttct ctagctgtta
     5341 agttaccttt gtctatgttt tcatagttag ataaacctat ttcgttagct atttctaatt
     5401 tcatttggtt taaagcagct tttgcttctg gaactactgt tctgtttgaa tttgacatag
     5461 taaatttcct cctcaaataa ttagtttatg atgtctaaca acacttttat ttatgtgatg
     5521 ttaatactaa tttctccaaa ataaaaataa tatatacaga aaaataaagg taaaaatgta
     5581 aaaaatataa aaaaattaaa taaatgagta ttttagagag tttaattagg tcaatatagg
     5641 ttttattttt ttagttattg tcttataaat taaatattac agtacttttt tgagctgaaa
     5701 aaaaataaat tttaaatata gaagaaatta tttcaaaggg ggaatagtat gaaaagagaa
     5761 gttaaaagga ctaaagcttt gatatatagt gttttatttt tttcattatt attagtgtta
     5821 atatttgttt ttgtttatgg aataaaatac atatgttcta aatctgacaa ttcaaaaatg
     5881 cctatatata aagttgatac aaatgagaaa aaaatagctt tgagttttga tgtagcatgg
     5941 ggaactgata atatggatga tattttaaaa attttagata aacataatgt aaaagctacc
     6001 ttctttttag ttggtagttg ggtggatgat aatgaagaaa ttgtaaaagc tatagatgaa
     6061 aaaggacatg aaattggaaa tcattcaaac actcatgcca atttgaagga aatatcaaaa
     6121 gaagacataa caaaagagat agaaactaca tcagaaaaaa tatataatat aacaggaaaa
     6181 aggactaatt tatttagacc tccttttggg gacgtaaaca ataaagctat ggacatttgt
     6241 agtgatttag gttataaagt aattaagtgg gatgtagatt ctatagactg gaaggagctt
     6301 ggaccaaatc atgtaattga aaaagtaata aaagaatctc aacctggttc aatagtttta
     6361 ttccatgcta atataaatga tgtagataac tatttggata ctataataag tcgactaaaa
     6421 aaagatggat acagtctagt caaaatatct gatttgttgt ataaagataa ttatgtagtt
     6481 gattccaatg gagtgcaaaa aactaagaat taataagtaa taaatgtata ttttaagtaa
     6541 agttgtgata aattatatag tatatgttag aaaagtgtcg ataaaaagac ggtgtctatt
     6601 ttttgacact ttttatacat gtatgcaata tcaataatta tagattaaat tgattaaaaa
     6661 agtgtctaat aattgacttt tttttgatgg gtttaaaaat ttagaagaat taatgaatgc
     6721 tgtaagacag tatttccaaa gggtataagt ttgtttaaaa gttggcatag aaattgcttt
     6781 gttaataagc gatatgttct atagaaataa acaatatgtt aagaccttat aatagaaaaa
     6841 tagcattaac attatttgaa cagcttataa aaaaattaat atacttaagg gggcgtacta
     6901 gttgaacaaa ctatattcta ttttattaaa acaacacgtt ggtggtccag ataagccagt
     6961 agtaagtgtt ggagatgtag taaaaaaagg aactctaatc gcagaaccaa ctggattagg
     7021 ggcaaatatt tatgccagtg ttagtggaaa aataagtgaa ataaatgacc aagcgatagt
     7081 aattgaagct gatgaagtac aagaagatac ttttgaacca ttaaaaggag aaggtatact
     7141 tgacttaatt aaagaagctg gagtagtagg aatgggtgga gcaggattcc ctactcatgt
     7201 aaaattaaac atagatttaa atggtggaac aatattagca aatgcagctg aatgtgaacc
     7261 tttattagct cataatataa aagaaataga agaaagacca gaaatagttt atcaaggtat
     7321 aaaatatgct atggaagtta ctaatgctgg aaaaggtatg ctagcaataa aaagtaagca
     7381 tccaaaagca atagaagctt tcaaaaaagt aataaagcca ggagacaaca tagaagttgc
     7441 agaattagtt gatatgtacc caatgggaga agagagagca atagtaagag acgttctagg
     7501 aaaattactt gagccaactc aattaccttc agaggctaat gcagtagtaa taaacgtaga
     7561 aacactaact agaatagtag aggctgttga gcaaaaaaga ccagttatat ctaaaaatat
     7621 aactgtagtt ggtcaattaa acagtggaaa agaatctata gtgtttgaag atgtgccaat
     7681 tggaacaact gtcggagaat taattgaaag agctggtgga ataaaaggtg aatatggtga
     7741 aataatccta ggaggacctt tcactggtaa agctactact ttagatgctc caataactaa
     7801 aactagtggt ggtataatag ttactatgcc atttgttaat gaaaagagaa aaatgggtct
     7861 tttagtttgt gcatgtggac ctaatgaaga aagaatgaga gatatagcaa ctaaaatggg
     7921 agttactgat atagtatcag ttcaaaaatg taaacaagct caagaaatta aaggtgcatt
     7981 aaaatgtgaa aatccaggac actgtccagg acaagctcaa aaatgtattg aatttaagaa
     8041 agctggagca gaagttatat taataggaaa ctgtactgac tgtagtaaca cagtaatggg
     8101 ttctgcacca aaattaaaat taggtgtata tcatattact gaccatgtaa tgagaacagt
     8161 taatcaccca ttaataagaa gaataaaagg tgatgtaaga ttaaaaataa aataatagat
     8221 aaatagaagt tagacatctt tagaagtaaa acttaaaagt tacttctaag gatgtttttt
     8281 aatgtatttt gagggcaata tatatattgt aaaataaatg gaaaaaatag acaaaatatg
     8341 ctagatattg tataatgata acaatagcta ttacaattaa tattggacaa aaataatgat
     8401 actcaagtat gtggtttgta ttaatatgca tattatatta agtcaactat ccgatagagt
     8461 aaaaggtttt tgtgatattt gaagatactc tatagtatct tttttattat aaaaactaat
     8521 ttttgtcttt tattagataa gtatctagga tactaattta tattttattg agttgttaaa
     8581 aaagtgcact taaaaagtta aaaaagaaaa attaaaaacc tatttataga aactgtgaag
     8641 tatatcttaa acctgggcac ttaaaagata tatggagtta gtagtgcaac ctgctataaa
     8701 tataaatagg agtaactttt aattgtcaaa aatatataaa ataaatacat aaataagtaa
     8761 ttaatagaag atagatataa atatattttt gaaatgctcc ctgcattcat aacgagtgta
     8821 gagtggcaga tatctgccca aaactccttt aattgctgga agttcctaaa gctaactaaa
     8881 ccacaacata atcatgaaat aaggataagt gtgatagagt gtgcatttaa taagcacttg
     8941 aggttacgaa agtagaaaaa attagttaga tagcataagg ttaaatccta agtgccttga
     9001 aaatggataa tcagcaggta agcttcgaaa agaagaaact tcaacgacta gagagaaatc
     9061 tcgtacctac aagtgtgggg aaatggggag tacctaattt atggttaggt aatgatatag
     9121 tctgtgctta atagaaatat taagaagttc ataggagaac tggttaggag taacgaccct
     9181 aattgaacac actaaagatg tattattgaa taagattcat ataatattta ataattttaa
     9241 ttatattaag tgaatacaat atatgttttt agtatcagga gaattttgaa aacaaaaatt
     9301 aaaaaatcaa gtataatcag tttagtaatt gcttttagta tggtatttac tgcatttaca
     9361 ccaatagtat cttatgctga cgaagtgact agcaatgata caatacttaa tggagaagaa
     9421 caatcaaatg gtcagacacc agatgtagag aaaccatcag acggtcaggt accagatggg
     9481 gagaaaccat cagacagtca agtaccagat ggggagaaac catcagacgg tcaggtacca
     9541 gatggggaga aaccatcaga tggtcagata ccagatgggg agaaaccatc agatggtcag
     9601 ataccagatg gagagaaacc atcagatggt cagataccag atggggagaa accatcagat
     9661 ggtcagatgc cagatggaga gaagccatca gatagtcaga cgccagatgt agagaaacca
     9721 tcagatggtc agatgccaga tggggagaaa ccattagatg agcaaactcc agaagaagaa
     9781 aaaccattgg aagaagaaat agttatagag gaaatgagtt taaaacaaga tattgataaa
     9841 attcttgata tgactttatc tcaaataaat aaaatagtat ataacttttg ggaagatgaa
     9901 gaagatgtaa aggcagatga acaatcagaa attaaccaag tttttacttc aaaagactca
     9961 tttattagct tatggtatga caaaaaagca aaagtaaaaa attcttgttt actaaaagaa
    10021 atagatggtt cagatagacc ttatcatgat ttaagatttg atgatattac aaagacttta
    10081 acttttgact atgtaataaa gggtcttgta ggagcaagct caaaagacga caaatatgta
    10141 atagaaggtg aagatggaga gcaaactgca ttttgttaca ataatcattt aagaccacca
    10201 agtagtaatg gaaagagtcc atatttacca gcagaagatt ttaatggtca agaaaaccaa
    10261 aatgaagaag cagttaaatc tatactatat gcaggttctg aatttgatgg atttggatat
    10321 aagcaacaat ttaatttagg tggagaagaa aatgaattga tgacttactc agctactcaa
    10381 agtgcaattt ggattattct tggacaaatg gatgaacaag aaagacttaa gcagtatcaa
    10441 ggttctataa acctttgtga taaacttata gaaagagcaa aaactgaaga ggaaagagcg
    10501 gagtatcaga agaagaaaga agtagctata aatataaaag cttatttaga agcattgtta
    10561 aaagcaggtc gtgaagagtt aaagccaaat gatacaggaa aacctagttt gagtaatggt
    10621 ttaactaaga tcaactttga aaaaaatgaa gatggtactt atgaaacaga agctgtagcc
    10681 ttagtaggtt atagtggtgt agtaaagtta agactaccag atggagtaac tgcttatgat
    10741 ggagatggta atataatagg tacagatgaa gtagaaattt caacacaaca gaaatttaaa
    10801 cttaagagtg ttggaaaacc agatgccaaa gctaatattt ctgctgtatc ttatgattat
    10861 atttttccaa aagctattca atattataag gcagtgcttg atttaggaca aaaagaccat
    10921 tcatcttcat taccatctag taagcaaaat ttattaagtt atacaataga gaaaaagaat
    10981 ggtcaagaag ttaactttaa tataggtcta ccaacagatg atgatgatgt tgtaaatcca
    11041 ccagtgccac caatagatga tgatgttgta aatccaccgg tgccaccaac agatgataca
    11101 aatggacata agccaaaacc atcaccacca atagatgata cagtaataaa tccaccagta
    11161 ccaccaatgg atgatacaat aataaatcca ccagtaccac caacggagga tgcagtgtta
    11221 aatccaccag tgccaccaat ggaggatgca gtgctaaatc caccagtgcc accaacagat
    11281 gatacagtgt taaatccacc agtaccacca gtaagtgata cagtggaaaa aactccagaa
    11341 ctatcaagag atgatacaat agtaaaatca ccaaaaacag gtgatgaaac tcagctaatg
    11401 tcatatgtat ttattagtgt cattgcaatt tgtggattag cgtatcaatg taaaataaaa
    11461 agaaattaat ctagattatt attaagttta aaataaaaag tctcaaattg atttataagt
    11521 cattttgggg cttttttatg taaatataat atgcaaatat agtttgttta aaaatatcta
    11581 ttttaattat attcttaaat ttatgattgg atacaggcat tcattaattg tttgatgaat
    11641 agaaaaatgt tttgaaggtg aaaatagtat tgaaaaaata atatactaag ggtataattc
    11701 atttgatttc atttgtataa atttaattat aaattttaaa gtgattatat aaatttaaat
    11761 gctaattact taataaattt taataataat tatttaataa ataattatta taatatgcta
    11821 gaataaatat atataaataa gttttgatga ggtactacta taaagggggg actttatgtt
    11881 ttcgatacca gaagtaaaaa aagtagaaga ggtaatggat acaaaattta caactataga
    11941 cgaagacaca agaattgaag atgcaataaa agaaatgata aaaagcaaca caaaaactct
    12001 aatggtaata gattcatctg accaattaaa aggcattata tccatgacgg atatacataa
    12061 tctttatgag atgcataaaa agtatgaagg tcaaccagta aaacttataa tgaagaagga
    12121 tgtaatatat gtcaacgaag gtttaacctt agatgaatgt agagatataa tgatattaaa
    12181 aaatataggg attcttcctg ttcttagaga taataaaatt attggagttc taaaacaaga
    12241 gcacattagg gactatctat atatgcattt agaagattat ggattaactt tgaagtatat
    12301 tataggtcaa attaaagaag gaatttgtgc tataaataac gaaggtgttg ttatcttgtg
    12361 gaataagttt atggaggaaa ggtatgatat aaagtctgaa gatatagttg gtcgaccgat
    12421 gaatgaattt ttggaaaata ctatttcaga aaaagtacta aatagtaaag ttggaatgag
    12481 tgacttgtat tttacagata aaaaagaaaa tatgtatgca ttagttcatg caaatcctat
    12541 attttataaa gaagaattta tcggtgttgt atgtacagaa gttgatgtta cagaagctaa
    12601 gatattagct cttgaacttg aaaaagtaaa tgatacatta aaatatctaa agaacgaagt
    12661 caaaaattta tctaaaggta gttttgataa gattttaggt aaaagttata aactagaaaa
    12721 atcgaaagcg attgcaaaac aagtggctag aacaaatagt agtatattta tttggggcga
    12781 aagtggtaca ggtaaagaag tatttgcaag agctattcat gattatagtg agagaaaagg
    12841 gcaatttata cctgttaact gtagtgccat accaaatgag ttatttgaaa gtgagttttt
    12901 tgggtatgaa tcaggtgcgt ttacaggagc aagtaaaaag ggtcgaatag gtatatttga
    12961 acttgcaaaa gatggaactg tatttttaga tgaaattgcc gatttacctc ttagtatgca
    13021 agcaaagttg cttagagtat tacaggaaaa agaaataaga agagttggtg gagataccac
    13081 aataaaaata aatcctagaa taatatcagc taccaataaa gatttgagta aaatggtaaa
    13141 ggccgaaaaa tttagagaag atttatacta tagattaaat gttgttgaaa taaagattcc
    13201 tccacttaga gagagaaaag aagacatagg attattggtt catagctttt tagaggagat
    13261 atgtaaacaa aataataagc ctgttcttac aattagcaaa gatgtaatag atatatttca
    13321 gaattataga tggaaaggta atattagaga attaaaaaat acgattgaaa atattgttgt
    13381 attgtcacaa aattctaaaa tagatgtgga tgatgtacca agttatatga tggattctac
    13441 aaataatagt actgaagaag aagagtatcc tttagattta acgaaggcaa ctcaaaaaat
    13501 tgaaataaag aatataacca aggcacttaa aatgtctaat ggaaataaag ctaaagcagc
    13561 taagatatta aatataccca gaacaacttt gtattataag atagaccaat acaaaataga
    13621 tgtgtctaaa atatgacaca tgactaaatt ttgacaaaat gataaacttt aatttaaaac
    13681 ctagtaaagc caacgcttgt ataagtgttg tgtatataga tgtgttaaaa aaaatgaaaa
    13741 agtgttgatt aattgacact ttttttgttt gagaaaatgt ttacaaatac aaaaaataac
    13801 atattttaaa taaatattta tataatattt ggctaaaaat agcgatagat gaagaatcta
    13861 ttatagaaaa aatctttttg ttaataagtt ggcataggaa ttgcttataa tataaagtga
    13921 ctttaagtga gaaaaaaata atattaatca taacaaatta tgtaggtttt aatttactta
    13981 atttctcagt taaggggtta cctgttgagg aattgagatt taaaataata aataagaaaa
    14041 taatgtaagg aggtatttta tactatgtca ataactttag aaacagctca agcccatgca
    14101 aatgacccag cagtttgttg ttgtagattt gaagcgggaa caattatagc gccagaaaac
    14161 ttagaagatc cagcaatatt tgcagactta gaggattctg gattattaac aataccagaa
    14221 aatggattaa ctataggtca agtactagga gctaagttaa aagaaacttt agatgcactt
    14281 tctccaatga ctacagataa cgtagaagga tacaaagcag gagaggctaa agaagaagta
    14341 gtagaagaaa cagtagaaga agcagctcca gtatcagaag cagcagtagt tccagtaagc
    14401 acaggagttg caggtgaaac agttaaaata cacataggtg aaggtaagaa cataagctta
    14461 gagatacctt tatcagtagc tggtcaagca ggagttgctg ctccagtagc aaacgttgct
    14521 gctccagtgg caagtgcagc agcagaagta gctccaaaag ttgaagaaaa gaaactttta
    14581 agaagcttaa ctaaaaaaca ctttaaaata gataaagttg aatttgctga tgaaactaaa
    14641 atagaaggaa ctactttata catcagaaac gcagaagaaa tatgtaaaga agctaatgaa
    14701 actcaagagt tagttgtaga tatgaagtta gaaataataa ctcctgataa atatgaaact
    14761 tacagtgaag ctgtattaga tatacaacca atcgctacta aagaagaagg cgaattaggt
    14821 tcaggtataa ctagagttat agatggagct gtaatggtat taactggtac agatgaagat
    14881 ggagttcaaa taggtgaatt tggttcttca gaaggtgagt taaatactac tataatgtgg
    14941 ggtagaccag gtgctgctga caaaggtgaa atattcatca aaggtcaagt aacaataaaa
    15001 gcaggaacta acatggaaag accaggacct ttagctgctc accgtgcatt tgactatgta
    15061 actcaagaaa taagagaagc attaaagaaa gttgacaact ctttagtagt tgatgaagaa
    15121 gtaatagagc aatacagaag agaaggtaaa aagaaagttg ttgttataaa agaaataatg
    15181 ggacaaggtg caatgcatga taacctaata ttaccagttg agccagttgg tacattagga
    15241 gctcaaccaa acgttgactt aggaaacatg ccagttgtat tatctccact tgaagtatta
    15301 gatggtggta tccatgcatt aacttgtata gggcctgcat caaaagaaat gtcaagacat
    15361 tactggagag agccattagt aataagagct atggaagacg aagaaataga tttagtaggt
    15421 gttgtatttg ttggttctcc acaagtaaat gctgagaaat tctatgtatc taagagatta
    15481 ggtatgttag ttgaagctat ggaagttgat ggagctgtag taactactga aggtttcgga
    15541 aacaaccata tagatttcgc atctcacata gagcaaatag gtatgagagg tataccagta
    15601 gttggtgtaa gtttctcagc tgttcaaggt gctctagttg ttggtaataa atacatgact
    15661 cacatggtag acaacaataa atctaagcaa ggtatagaga atgaaatatt atctaacaac
    15721 actttagctc cagaagatgc tgttagaata atggctatgc ttaaaaatgc tatagaaggt
    15781 gtagaagtta aagctcctga aagaaaatgg aatccaaatg ttaaattaaa taacatagaa
    15841 gctatagaaa aagttacagg agaaaaaata gtattagaag agaatgagca atctctacca
    15901 atgagtaaga agagaagaga aatatacgaa aaagacgaaa actaaaattt agatactaac
    15961 taagatttag acaataacat aaatttagat ccaagtttaa aacagtatag agaggtagaa
    16021 ttataatgga taggttaaaa tatatctcat cagagacttt ttatgaaggt attatagttg
    16081 acatcaaagg cggtggagtg actatagacc taaaaggtag acttggacaa tttaaaatac
    16141 ctaatagaat gcttataact gactacgaac ttaagatagg tcaagaagtt ggattcatgc
    16201 taagctatcc tgaggtatta agtccagagc ctaatcaaga gtatgtagaa aatattagga
    16261 gagaaaaaga aaaacaagca gaaatgcaat caaaacaaga agaaaaataa taaataatcg
    16321 tttgaaaata gaagggagag gaaatatatg agccttacaa cagtacaagg acttcaatct
    16381 gaaatattcg ttccaataac acctcctcca gtttggactc ctgtaactaa agaattaaaa
    16441 gatatgacta tagctttagc tacagcagct ggtgttcatt taaaatctga caagagattt
    16501 aatctagcag gagatactac atttagagct atacctaata cagctacagt tgatgaaatg
    16561 atggtatcac atggtggata tgacaatgga gatgtaaaca aagatataaa ctgtatgttc
    16621 cctatagata gattacatga attagctgca gaaggattta tcaaaggagt agctcctatg
    16681 cactatgcat tcatgggtgg tggaggaaac caacatgtct tcactgaaga aactggtcct
    16741 gctatcgctg ctaaacttaa ggaagaggga gtagacggtg tagttatgac agctggctga
    16801 ggtacttgcc atagaactgc cgtgatcgtg cagagagcaa tagaggaagc tggaatacct
    16861 acaataataa tagcagctct tcctccagta gttagacaaa acggaactcc tagagcagtt
    16921 gctccattag ttccaatggg tgctaatgct ggtgaaccac ataatataga aatgcaaaca
    16981 catatattaa gagatacatt agagcaatta gttgcaatac catctgctgg taagatagtt
    17041 ccattaccat acgaatataa agctcacgtt taaaatgtaa tttatatttg aaaatataga
    17101 acccttctcc tacacggtta ggagaggggt tttaagaaca aaggtggata ttatggaaga
    17161 aaaaataatg agaagacttg ttataaaacc gtttcatatg aatgaagtta atttcggttc
    17221 aaaaacttct ataaagaaag atgtacttac aatagattta tcaagtatag atgaaataaa
    17281 agaaagagaa gagttaataa cagacataaa agtagacatt ataaaacctg gagattatga
    17341 cagagaaatt aatactatta tggacattat accaatatca acaaaagttt taggcagact
    17401 gggagagggt attacacata ctttaacggg tgtatatgta atgttaactg gtgcagatga
    17461 agatggaaat caaatgcatg aatttggttc ttcagaagga attttgaaag accaaatggt
    17521 atttgggaga tatggaactc catcagttga agattatata atccatgtag atgtaacttt
    17581 aaagggaggt ttaccttttg aaagaacact tccacttgca gcattcagag catgtgatga
    17641 ttttatacaa gaaataagag cgtctttgaa aatggaagac ggaagaaatg ctactcaagt
    17701 tcgtgaatat tttgacaaaa ttcgaccaaa tgcaaaaaag gttgtcatag taaaacaaat
    17761 agcaggtcaa ggtgctatgt atgacaatca gttattctca aaggaaccta gtggatttga
    17821 aggaggaaca tcaattatag atatgggaaa tgtacctatg attatatctc ctaatgaata
    17881 cagagatggt gcccttagag ctatgactta agagaggtgg atattatggg aataggacct
    17941 tcaacaaaag aaacatctct tcatcacttt agagacccac ttttagacat agttgaaagt
    18001 gataaggatg tagatttact tggagtaata gtagtaggaa ctccagatgg aaatgaaaat
    18061 aaaacctttg tgggacaaag aacggccgct tggctggaag ctatgagagt agatggagct
    18121 atagtttcat cagatggttg gggaaactca catgttgact acgctaacac atttgaggaa
    18181 ataggaaaaa gagatatacc tgtagttgga gttacattta atgggactca agctaagttt
    18241 gttgtaagca atcaatatat ggataccata gttgatatga acaaatcaaa agaaggtatt
    18301 gagacagaag tagttggaga aaacaacaca aatgaaatag atgctaaaaa agctttggca
    18361 tttttaaaac ttaaaatgag aaaacatgga taaaaaattt aagtaaggga atgagttttg
    18421 tgggaatttc tacatcaatg aaaaacacat cacttcatat atttagagac ccgttactag
    18481 atttgataga taatgattct gacatagact tgttaggagt ggttgtagtt gggacatcag
    18541 agcacaatga atggaaaaca tttttagcaa ctagacttgg aagatggatt gaatcattgc
    18601 gacctgatgg agtgattata actttagata tggcaggaaa ccagcacatt gattttacaa
    18661 atgctatagc agagtttgta aaatcagata tacctacagt aggtcttact attatgggag
    18721 cagatggatt agtaattacc aatccatatt tggataaagc taccataatt gattataaaa
    18781 agacaacagg ctacatagaa acagaagtag ttggagacaa tcacatggat gaagttgatg
    18841 ttaaaaaagc attggctttc ctaaaattaa aaatgcgtaa agacgcaaaa taaacctaag
    18901 ggggaatttg atatgaaatt tagcagaagt atacaagcta tagactctca tacagcaggt
    18961 gaagcaacta gaatagttgt aggtggaata cctaacataa aaggaaattc tatgcctgag
    19021 aaaaaagaat atttagagga aaatctagat tatttaagaa ctgctataat gttagagcca
    19081 agaggacata atgatatgtt tggttctgta atgactcaac cttgttgccc agatgctgac
    19141 tttggaataa tcttcatgga tggtggcgga taccttaaca tgtgtggcca tggtacaata
    19201 ggagctatga cagcagctat agaaacaggt gtagttccag cagtagagcc tgtaactcat
    19261 gtagttatgg aagctccagc aggaataata agaggagacg ttacagttgt agatggaaaa
    19321 gctaaagaag tatcattctt aaacgtacca gctttcttat ataaagaagg tgttgaagtt
    19381 gacttaccag gtgttggaac tgttaaattt gacatatcat ttggtggaag cttctttgct
    19441 ataatacatg caagtcaatt aggtttaaaa atcgaacctc aaaatgctgg taaattaact
    19501 gaactagcta tgaaacttag agatataata aatgagaaaa tagaaataca acatccaact
    19561 ttagctcata taaaaactgt agacttagtt gaaatatatg atgagccaac tcatccagaa
    19621 gctacttaca aaaatgtagt tatatttggt caaggtcaag ttgacagatc tccatgtgga
    19681 actggaacaa gtgctaaatt agctacttta catgctaaag gtgaattaaa agtaggagaa
    19741 aaattcgtat atgaaagtat attaggaact ttattcaaag gtgaaatagt tgaagaaact
    19801 aaagttgcag atttcaatgc tgtagtacct aaaataactg gttctgctta tataactgga
    19861 tttaatcatt ttgtaataga tgaagaagac ccacttaaac atggatttat tcttaaataa
    19921 gtaataaata aattaggaaa ttaaaataga atagtagcca cttgtcggtt gctattctat
    19981 agcttttagg ctataagatt cagaatatat gaaaaaataa aaaaatagga ggatggatta
    20041 tggtagcaat cttaaaggca ttatatgtag tattcgcagc aatctttgca gttgtgtttg
    20101 gtaaagacgt agctaaagca aacaaagagg ggaaactaga agaacaaagt actccaaaag
    20161 ttgctgtaac aggatttata acagacttct ttgatacatt aggaataggg tcatttgctc
    20221 caacaatagc aatgtcaaag gctttaaagt taaatatacc agataaaaaa atgcctggta
    20281 cattaaacgt agcacatact attccagttg ttacagaagc attcatattt acatcaatca
    20341 taccagttga tggtgttact ttagtatcta tggttgtggc agcagcagtt ggttcatata
    20401 taggtgcagg aataatagct aagatggacg agagaaaaat acaacttgtt atgggtgtaa
    20461 ctttagcatt aacagcaata ttaatgttat taggtcatcc ttggattaac gtattaccag
    20521 gtggaggaaa tgaattaggt cttactggtg ttaaattagt aataggtgta ataggtaact
    20581 ttatactagg agctttaatg acagctggta taggtctata tgctccaggt atggcaatgg
    20641 tttatttctt aggaatgtct gcaaaagttg cattcccaat agttatgggt tcttgtgcat
    20701 tattaatgcc agttgcaagt atgaaattca taaaagaaga tgcatatact aaaaaagcat
    20761 cagttataat agctatatgt ggtttaatag gagttttcgt agctgcatat atagtaaaaa
    20821 ctctaccaat ggatatatta aaagcattag taatagtagt tatagcttat acttctgcta
    20881 caatgataat ggctgcaaat aaaaacaaaa agactgctta gttaaagact acttagtgtt
    20941 tattaaatat tacagctaat aataaagatt ataaactgct ttaaaatatt gaattaatat
    21001 tttagggcag ttttttattt actaaattaa tataaagcaa ttaacatgcc aattatgtaa
    21061 tatatatagt gatttagtag atttaatgta aaaaacgttg tattttt
//
LOCUS       DAEMIK010000070        20702 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEMIK010000070 DAEMIK010000000
VERSION     DAEMIK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.070"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            59..916
                     /locus_tag="KPK87_002115"
     CDS             59..916
                     /locus_tag="KPK87_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2538264.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVGNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLSDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            1013..1882
                     /locus_tag="KPK87_002116"
     CDS             1013..1882
                     /locus_tag="KPK87_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2538265.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(2252..2884)
                     /locus_tag="KPK87_002117"
     CDS             complement(2252..2884)
                     /locus_tag="KPK87_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG2538266.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(2956..3309)
                     /locus_tag="KPK87_002118"
     CDS             complement(2956..3309)
                     /locus_tag="KPK87_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG2538267.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(3387..3590)
                     /locus_tag="KPK87_002119"
     CDS             complement(3387..3590)
                     /locus_tag="KPK87_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2538268.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            3655..3792
                     /locus_tag="KPK87_002120"
     CDS             3655..3792
                     /locus_tag="KPK87_002120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538269.1"
                     /translation="MVFQPIPFLWAEPCHTGVSLYCGTGYGKTTVPKVIHKSLYSSSL
                     D"
     gene            complement(3980..4210)
                     /locus_tag="KPK87_002121"
     CDS             complement(3980..4210)
                     /locus_tag="KPK87_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538270.1"
                     /translation="MKPTKEVLPFTIILLAVHSDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(4212..4619)
                     /locus_tag="KPK87_002122"
     CDS             complement(4212..4619)
                     /locus_tag="KPK87_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2538271.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRINDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKKLKELMEEH"
     gene            complement(4978..5427)
                     /locus_tag="KPK87_002123"
                     /pseudo
     CDS             complement(4978..5427)
                     /locus_tag="KPK87_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(5441..6367)
                     /locus_tag="KPK87_002124"
     CDS             complement(5441..6367)
                     /locus_tag="KPK87_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538272.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(6492..7274)
                     /locus_tag="KPK87_002125"
     CDS             complement(6492..7274)
                     /locus_tag="KPK87_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG2538273.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(7430..8050)
                     /locus_tag="KPK87_002126"
     CDS             complement(7430..8050)
                     /locus_tag="KPK87_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2538274.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(8138..8836)
                     /locus_tag="KPK87_002127"
     CDS             complement(8138..8836)
                     /locus_tag="KPK87_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538275.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAKRGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            8937..9752
                     /locus_tag="KPK87_002128"
                     /pseudo
     CDS             8937..9752
                     /locus_tag="KPK87_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            10422..11189
                     /locus_tag="KPK87_002129"
     CDS             10422..11189
                     /locus_tag="KPK87_002129"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG2538276.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(11522..12199)
                     /locus_tag="KPK87_002130"
     CDS             complement(11522..12199)
                     /locus_tag="KPK87_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538277.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            12623..15667
                     /locus_tag="KPK87_002131"
     CDS             12623..15667
                     /locus_tag="KPK87_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2538278.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTASHNEISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDNSGNTEVAEVKATTVYIKELTAPMGFKVDKT
                     IYSLKVEAGKTATLKVSDTPKVADTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYTKDNLPAEATRTWVTKTIAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTCEETKSPTGYLLDGAYMKANGSEEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFAITENGKIVDLTDEAHSV
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDEQTVLITERIIKIHTTATDKDSNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            15903..16229
                     /locus_tag="KPK87_002132"
     CDS             15903..16229
                     /locus_tag="KPK87_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2538279.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            16244..16627
                     /locus_tag="KPK87_002133"
     CDS             16244..16627
                     /locus_tag="KPK87_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2538280.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQAGNPQNHIPQGQPKK"
     gene            16725..17210
                     /locus_tag="KPK87_002134"
     CDS             16725..17210
                     /locus_tag="KPK87_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538281.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            17327..18721
                     /locus_tag="KPK87_002135"
     CDS             17327..18721
                     /locus_tag="KPK87_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538282.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            18923..20122
                     /locus_tag="KPK87_002136"
     CDS             18923..20122
                     /locus_tag="KPK87_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2538283.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLVAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIQKCEHEECISKFRSFKSYGSGELVKHNETDKYD
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHHQIIKQQTATVEE
                     MVANVKE"
     gene            20129..20263
                     /locus_tag="KPK87_002137"
     CDS             20129..20263
                     /locus_tag="KPK87_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2538284.1"
                     /translation="MWELLKDLLLVSFGIGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            20524..20676
                     /locus_tag="KPK87_002138"
     CDS             20524..20676
                     /locus_tag="KPK87_002138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538285.1"
                     /translation="MGSVSNRSGVYKISMVRMFHKGLTKQRMYGSKFVLGRNAKVREI
                     VAGVAK"
BASE COUNT         7110 a         3402 c         3763 g         6427 t
ORIGIN      
        1 aaaacaattt acttaggata aactataata gtattcaaat attatggggg ttagatttat
       61 gagtaaagat gtaaaggtaa agcgtaataa gaaatatgat acatttagta taactatgga
      121 acttgagaga cctttacttt atgatgaggt tgagaacaaa gatgataaaa ataataatag
      181 aaaatttaac ataaagaata agatatttaa gggaataggt atactatttg tattgatttt
      241 gcttggcttt ttcatatgga taaataaaac atatgaacct caaaagctgg caaaagaagc
      301 tctagtcggc aatagtaaag ttgaagtaac tgtaaatgaa aacatttcat ttacaccaaa
      361 aggaagaaaa gtttctaaag gtttaatttt gtatccagga gctaaagtag aaatagaatc
      421 ttatgctcca cttgcaagaa aaatagcaga aagtggatat gaggttgtaa tcgtaaagat
      481 gccacttaat ctagctatac ttggaactaa taaggcacag aaagttatgg attcttacaa
      541 caacatagaa cattgggtaa taggaggtca ttcattagga ggagttgcag catcaaattt
      601 tgcacgtgat aataaattaa ttgaaggcgt tgtattttta gcttcttatc caatgggtga
      661 tgagttgaaa gaattaggaa aaaaagttat atctatatgg gggagcaaag atggagtagt
      721 caattttaaa agtcttgtag aatcaaaaca aaaactttct gatgatacaa cttatgttga
      781 gattgaagga ggaaatcatg cacaatttgg agattatgga aaacaaaagg gagataatga
      841 tgctattata agtcaagaaa aacaacttaa tattactgcc aatagtatca ttaaattttt
      901 aaagaatata tcttaaacta aaatttaaaa aatataattt tataagttat tatttaaagt
      961 aaatattaat aatataatag aatgaatatt ttattacaag ggggatttta atatggaata
     1021 cgatatgcca ctttacagac caccaagtga ggcttatagt cttataatac aagtcacact
     1081 aggatgttct cataacaaat gtacgttttg tagtatgtac aaatctaaaa aatttaaaat
     1141 aaagccacta gaagtaataa aaaatgaaat tgatatattt agaagatatt ataagcatgt
     1201 agaaagaata ttcttagcag atggagatgc attgataata ccaatggaga aattaagaga
     1261 tattatttta tatataaaag aagtttttcc agagtgtgaa agaattacta tgtacggctc
     1321 tcctaaatct atagagaaaa aatctgatga tgaacttaaa gaattaagaa aacttggtgt
     1381 taaaatgatt tatctagggc ttgaaagtgg aagcaatgaa gtattagaag atattaagaa
     1441 aggttttagt agtgaacaat taataaaagt tggtagaaaa gttaaaaaaa gtgggattaa
     1501 attatcagcc actgtaatag caggtcttgg aggaactaaa aaaacacatc agcatgcagt
     1561 tgatacagga aaaatgttag gagcaatttc accagattat gtgggtgtac ttagtttaat
     1621 ggtagaacct aatactgaac tttacgattt acttaaggca ggagagttta caatactaga
     1681 ggataaggct gtgttacaag aaattaaaga gatgataaag aatatagatg ctaatgaaaa
     1741 aattgtattt agaagtaatc atgcatcaaa ttatgctaat ttaaaaggtg tattacctga
     1801 tgataaacaa cgtctaattg atgaaattga ttactatcta agtaaccaaa agctaaaaag
     1861 agaagaatat agaagactat agtttttgat atatcaatct atattgatag gtaaatacta
     1921 agattattta gatacaaaaa atcatgctac attaatagtt gcttactatt gatgtagcat
     1981 gattttttat taaaaaacaa aatggtatat tcatatataa tatatcaata ataatgaaaa
     2041 tgtgaactag tttaatagaa ttttacctat tataataaaa acaggaattt aaataataaa
     2101 aagtaaagta actttttatt aagtaacttt actttttatt ttatttaatg ttgtattaaa
     2161 tttatatata ttaaaataat atacaaattt tgaaaaagct cttataaaat gattattaaa
     2221 gtaaagttta atataaataa attataaaat attaaactaa tttatcaata tttcctgatt
     2281 gaatatgctt aatgtttgat tgaatttttt tatatggcca atcccaccat ttaatttcta
     2341 ataattttga aattgtatca ttattaaatc tcttttttat aatttttgca ggtactcctc
     2401 caacaataga atacggtgga acgtcattag taactactgc tcttgttcca ataattgcac
     2461 catcaccaat atttacacct gacataatta tagaatcata acctatccac acatcatttc
     2521 caattataat gttaccctta ttgtcccaag cgtccgttat atggttgata tcgagtttcc
     2581 attcttcata aaagatggga aaagtatatg tggattttga tttcattgag tgatttccac
     2641 tagtcattag aaattttgca ttacatgcta ttgagcaaaa cttaccaatt attaatttat
     2701 cattgtttat agggtaatga tacaatacat tgtttttctc aaaatctctt gggtcattat
     2761 aaaagtcatt atatattgtg taatctccaa cttctatatt ttcccttgtt attacatttt
     2821 taagatatac agtttgataa tcattacttc ttggatatac cttatttgaa cttggaatag
     2881 tcatatttaa atctctcctt taatatatat tatgtttttg actattccac tactacaata
     2941 catctcttca cagaatcact ccttgttaag aatcttcata agataatggc gtcctttttt
     3001 agtgttaagt cggatattct ttcttagtac attcttttta gcgtataact gacaaactgt
     3061 cattttctta ccaatagtgt caatgctatc atcaaggtca ttttgtattg ctttttcctt
     3121 atcccttaat gatatatcat cacgttttcc ctttgggggt ttatcagtag gtacaagttt
     3181 ccaagagtaa acaaattgtg gtttaccaaa tgaatataca tacttatatg cgtatcttcc
     3241 atcttttctc tggctctctc cagttcttaa aattctatct ttattatctc gtcttttttc
     3301 agttgacatg ataagaagct cctctcaata accgaattga gcttgatacg tcatatgaat
     3361 attataacat ataaggctca atttcattag ataaagtcaa gtgtatcaac gattttctca
     3421 aattgttttc tctttatttg aatgcgattg ctattcataa tgacccagcc agaatcaagg
     3481 ttttcatcag caagtttacg taattttttc tctccaatac gaaagtattt tgacgcttct
     3541 tcaatcgtca aggtatattt ttcccaaata ggtacatcag tattgttcat agaatgtatc
     3601 actcctttat atgttcgtag ttcagaagta gatagatggc tttggtaagc tccattggta
     3661 tttcaaccaa tcccattctt atgggcagaa ccgtgtcata caggtgtatc attatactgt
     3721 ggtacaggtt acggcaaaac aaccgttcca aaggttattc acaaatcact atatagtagc
     3781 tctcttgatt aaggtcatgg cgtatagtat ttgttggcta tctgtatcac aaacgtatct
     3841 atctacttta ttcagttgtc aaagaacagt cggcattgat ttgcctataa aaaatcacaa
     3901 tagcttgtgg gcttttatag acaaataatt agtgattctc catgtaccaa atagcttgat
     3961 acatggagag agggcgagat taagacaaat taaatctcaa aatagcagtg attagttttg
     4021 ttcctagcat tcgctttgtt tcttcttcta catgaatata gggatttccg tattcatcaa
     4081 ataaagtttt ctgtgaaaga cgaatgatat agtgttcaaa atgtttaagt attccgttta
     4141 ttgcatttac atcgctatgt acagcaagta aaataattgt aaaaggtaat acttctttag
     4201 taggtttcat ttcaatgttc ctccatgagt tctttcaact tttttagtgc atttgttttg
     4261 tgataatgta tggtattttc tgctaaatcc atcaatgttg ctatgtcagc atttgtcatg
     4321 ttgagaaaga atgaaagtag aattatatct tgttttcttt ttgtaagttt tccgattgct
     4381 tgtgccatta atacatcttc aattcgtatg ttgtagtcgt aagaaaccat acagatgtag
     4441 ttagaatcat aatcatctaa aatactcaat ttacttaatt ctgcaagtga caaagattcg
     4501 agagatacca aataatcatt tatgcgttta ttttcgtcat aaatatctct agcatagtta
     4561 cgcaaaactg ttttacagaa actgtcgaat tgatgttcaa ttgtcttttc atcaaccata
     4621 agatttcacc ctctttccat cgcaaggagt taaataacct tgcttactcc tatcacggtg
     4681 ggagggcgtt aaatcttcga taaatttcaa aaaaagagaa aaaaatacac actctgccta
     4741 tagggcaaaa atgtgtattc tttaaaacgt atttaattta tattggttgt aagccaccac
     4801 catattgatg tatggaaata tgcattggct agcttttcat aaaaatacaa agatgcataa
     4861 gaaattaata gaggggaaaa acttattctg ttattaaaaa gtaggttaat tctaataaat
     4921 gctattgcgg ttgaattaaa ctatatacat ggcaatcaat catgtttcta ttctttatta
     4981 tgaattgtga cagtattctt caatcttaaa accacatttt tccaatactt tacttgaccc
     5041 tctattgtag gaatatacat tagcatgaat tttgttaagg ttttattttt caaaggcaat
     5101 tttgcatatt tcttttactg catttgttgc tatccctttg ccccaatagt tcctagcaag
     5161 ccagtaacct aattcagcat ttttattaat agaggtatct tcttcaaatg ttaggctgat
     5221 acttccaata gccctttctt gataaaaaat agcatagcta agttgtttaa catctggagt
     5281 tgccaaacaa aaactaataa aatctctagc gttttgctcc ctataagggt gggggaagtt
     5341 gtcacgaaga ttctttgcaa tttctttatc attcgcaaac tcaactaaat cggtaatata
     5401 agcataattc cactctataa gttccatttt tcttctcctt ttatgaaaca gctacaagaa
     5461 atctttctaa gccaatacca cttatcaaac agcgttcttt tttatttcca agcatttgat
     5521 aagtccagtc aacaaatcca ccgtcaacta tttcaacggt ttcatttcct tgtttgaccg
     5581 ataatttgaa attgatacct ttataataat tattatcaac atcatcatta tgaattgata
     5641 actctatatt aggaaaaata agtttgatag tttcaaccat tctgtcgaaa aatccgtcat
     5701 tatctttgta tccgtttctt ttgcgtagaa taatagagaa acgaacattt tctatttcac
     5761 ttaatatatc tttataatag tttaagtgct tttctaacaa cattttttca caagtgtaag
     5821 accctgtatc tataccagat gaaaccatac aatataagcc aaaatgtgaa tataaacctt
     5881 tacctacaaa attttgtgct cttattaccc tagcagtagt agccatgtgg ataggtatgg
     5941 tattattttc ttcttttctt tttaatttat cagcaataat tattgccaac atatttgagg
     6001 ggtctgcaag tgtttctgtc cctcgtagag cacttactac attatattga tctacggagc
     6061 caaaaacgct acagctacct aatggagcag agggagaaag cataatcgtc ttgatatcca
     6121 tattgaatgc ttttctcaat aattttgact ccaaagcatg aatttcttct ggatctatac
     6181 cgctagggat agtaaatcta tttagttggt atgactttag aatatcagtt gcagttaaag
     6241 tatttgcttg tctatcaaac acttctaata gaagtgagtt caaatctgat tttgatagag
     6301 ctgataactt atcaactata ttcttatccc caattttatt tgtaattcta cttaaaatat
     6361 cactcatagt tatcctccat actgttactt tcaaaataat tatagaattg ttacaacaac
     6421 taatctaggt catttgacca aaaatgaaaa aacactgaat atttactatt caatattttt
     6481 ttactttggg tttatttgat taaatcttga aaatactgta atgcaaccct ttcagcttca
     6541 acctttcgga caataggact ttgtgataat tcctctctta actcaagata caggttgata
     6601 gtagcctgca atgcattaac acagctttta tattcatggt tagcaaccgt cttttctaat
     6661 gcttcaatat attggggtgc gtccaattca atttttctaa ctccacgagg taatttacct
     6721 aagagaagct gtattaatgg ggcaagtact gattgtctta aaaaagataa aaaatcaatt
     6781 gtttcaaata gttctctacg tccaatctta gtgcaagcgt aatgaaccca aatccaaaat
     6841 ctatcttcaa tccactgtaa gtctggagtt gggaagtatg cttctttttt agaataaatt
     6901 tctgttatgc agttgttttc ttcatagaga atagctgaat cttcaaccct atctagtaaa
     6961 ccatctaagg ttgtaaactt aaaatcaaca tgaagaacag gggaatcata gagggaaatt
     7021 atcaatcgag gttctccaac gtgttcgcca gtaaaagcag aaactaatgt tccaaactgt
     7081 tccacaataa gtaatctctc ttcgataact tcttcatagg atttatcatc tgctacaatc
     7141 agaaaatcca aatcagaata ttcatctaac gattttgtga tataagaacc gctaattgca
     7201 agtcctatta tcctactatc tctttttgaa aattctaata cgttttgtat cattttctgt
     7261 tgtgttttag tcataatata acacctctca ctgtctttta ttagctatca atccaacata
     7321 attatgcata ggagtattat ataatgaaga aataaaaaaa aataggtcac ttgacccgaa
     7381 ataaaaaagc attgaataat tattattcaa tacttttttg aaattatact tatgttgtag
     7441 gaagattctt taatacctct cgctgattaa ttacactaaa tcttttgttt tcataagcaa
     7501 ttagatgttc ttgtacacat tcatttatag cacgattaat gcttctaatt ggagcgttta
     7561 cttcgctagc aagctgtttt ttggtaagca gatttaattc acttctataa tagtgtaagg
     7621 atatacacct cagaagtcgt tctttaacac ttaaagatga taattcttca attaattcag
     7681 catttacaac taatttacta gatatttctc gaatcagaaa ttttgtagct tcaaaatctc
     7741 cccttaacca gtttaaaaaa tcatctctat gaagtttata tacattacaa tctgtgaaag
     7801 cgacaatttc aacgggtttt ttcccatcat taaattgttc catttctcca aaaaaactac
     7861 cacttctata aagatgaatc gttgagatgt ttcctttcac atttaagatg aatgcttctg
     7921 caataccgtc aatcataaga attacatatt cattttcttg ctcagcaaaa agaatagtct
     7981 ctttcttttt atacgttttt ttaacaagct tctttttaat atcttcattc ataaaatcaa
     8041 ttaaatgcat attcatccct ccttaccaaa tctttgatta aattatatca tctttcaatt
     8101 agttaggtaa gatagtaaaa atatatttac atagaaacta ttccattatt attttccagt
     8161 tgtcattttt ctgcaaaaac ttatcactgt cacgactgga gataccagta aggaatgtta
     8221 ggatatcaat cgctaaactt tcagaatcct ttttacggct caaaatgacg taagggtcaa
     8281 gcaacccttt gttttcatca ttactggtta aattgacaat gtttatctca tgggctattt
     8341 ctggtagcgt ttctttccat ttgccgcgtt ttgctttagg gtcaactatg accgcctgtc
     8401 cgccgaatag taccgcatag taaacaagca tattgttact gaataattta ccaccaccca
     8461 atgaaccgat aaacgcagac gctaacgcat tggtaataga accctttacc ccttgactag
     8521 caagggcagg ttttagatag acattctttc cagtgtccag attgtagcca agatagatat
     8581 cctcatgttc gccaagggtt tgtgtcgctc caaaaccaag tccagccaaa aaatccgaag
     8641 tgacatattg aatataatca ttgatgtaac gtttgctgac aggaataaat tcgccatgca
     8701 atcccaacat atccccaaag ggacgcacga gcttgattga taagtcgtcg taaaagtctt
     8761 tgacttcatc acaacgcctt tttagctcgt caagattctt tgtagataca cggacaacat
     8821 agctaagttt atacatagat tctttacagt taaaagtctg tttttaaaaa aatgtataaa
     8881 ttataaatca aaatatagat actatcaatg acccactagg tgcaaaaagc attgatatgg
     8941 ttaaattatc agacgctaaa agctgggcta tccgaatgag tgaagatggg tgtgcttata
     9001 aatccattga caattataag cgctcactga aagcgtcgtt tttcatggca attcaagacg
     9061 actgtattag aaagaatcca tttaattttt agttaagtga tgttttggaa gatacaacag
     9121 aaccaaaaat cgctctaaca ccagaacagg aaatgggctt attatccttt atggggagcg
     9181 ataagactta ttgtaaatat tatgatgatg ttgttatcct cttagaaaca ggactacgca
     9241 tttctgaatt atgcggtttg actttagaat tggatatgaa aaataaagcc attatggtag
     9301 accatcaatt gttgagtgat actaagattg gctattatgt agaagttcca aagaccacac
     9361 agggtaagcg agaaattcca atgactgaaa gagcttatca atcatttgaa cgtatcgtgg
     9421 aacggcgtaa gaacgctaaa gcagaaccaa tcattgttga tggttaccag aacttcctat
     9481 ttcttaaaca agatggatta ccaaaacaag caactcatta tcaaagcatg ttaagagcgt
     9541 tagtcaagaa gtataacaag acccatgagg acaagctacc aaagattacg ccatatatct
     9601 ttatgcatac actctgtaca aatatggcta acaaaggaat gagtcctaat atattgcaat
     9661 atattatggg gcataaaaac attactatga cgcttggcta ttatgcacat ggttcattta
     9721 gttctgcgag agctgaaatg gaacggttat aagcctagta gtatgactag agtttactac
     9781 ttatttacta cttttgatgg cattcttata cggttaaatg ccaacttatg caaagtatct
     9841 tccaaagaga aaatgccgat actccttgta gatacaggat atatcgacat ttaccaactt
     9901 atgaaaagat aatcaaattt aatataagtt tttcaaataa aaagatacta aattcttttt
     9961 agagaatcta tataacatta cttaagcttg gttggggtat aaacaattga gtagctactt
    10021 ttgtatggtg ttcaacttta gtcaaaactc ttaaataatg caaatgattt agattcatta
    10081 atattcaatc tcttcataag tagtgaaatt cgagttattt ctgcataatt atactatgat
    10141 ttatagatta attctattag catccagaat aatattgttt atatgagtaa aatttaaaga
    10201 atattgtgaa aattatagaa tgttaaaata aatttaggta aaaaaagtgt ttgtatatta
    10261 tagagtacct tttctaaaat atgtgggatt gttttaagaa ttagaataaa aattattttt
    10321 aaattagtat ttttataatg tatagtattt ttaatgtata attaaattgt gcagaaaata
    10381 gaatttaaag tcctatagaa tttctgatta tggaggagaa tatgaaaaga aaagttcaag
    10441 taaaaaatat tactattgga gaaggtagac ctaaaatttg tgtaccaatt attgggaaaa
    10501 ataaaaaaga tattataaaa gaggctaaag agttaaaaga tgcatgtttg gatattatag
    10561 agtggcgagt tgattttttt gagaatgtag aaaatattaa agaagttaag gaagttctct
    10621 atgaattaag aagttatata catgatatcc ctttattatt cacatttaga agtgtagtag
    10681 aagggggaga aaaattaata tcaagagatt attatactac tttgaataaa gaaatttcta
    10741 atacaggttt agtagattta atagatgtag agttatttat gggagatgag gtcatagatg
    10801 aagtagttaa ttttgctcat aaaaaagaag tgaaagtaat tatatctaat catgatttta
    10861 ataaaactcc aaaaaaagaa gagattgtat ctcgtttatg tagaatgcag gaattgggcg
    10921 cagatttacc caaaatagct gttatgccgc aaaatgaaaa agatgtgtta gtgctacttg
    10981 aagctacaaa tgaaatgttt aaaatttatg cagatagacc aataataaca atgtcaatgt
    11041 caggaatggg tgttataagt agattgtgtg gtgaaatatt tggttctgca ttgacttttg
    11101 gagctgcaaa aagtgtttca gcaccaggac aaatatcatt taaagagtta aattcagtac
    11161 ttaatctatt acataagagt attaattaaa ttaattatat agaaatatta gaaggtattt
    11221 tatttttgaa gatataaatt aaatataaaa aacttttcag atagtaaaat ttaaattggg
    11281 caattatatc tgaaaagcaa ctatttagtg tgtattttga aagtgatata gaaaatctta
    11341 taaatcaact atggcatatt taaaatatgc cattaaaacc tactagacat tagattatag
    11401 atggtatact agcatttcta ttatttatag atagtaagtt ttcataataa ctgttttatc
    11461 taaagtagaa gatatatttg tattatgtat atttgtcatt atttttttgt aattatgtat
    11521 attaatggtt atatatgtaa tataatatta tcactctatt ttttgtatat tgtacattac
    11581 aacatttata tctatcatca tcaattacag tagaatgtat gggatactta ttttcactta
    11641 accaatattt ttgcatattc tttgtccaag actttggaaa cttcaatact tttttgttta
    11701 ttataacatc ctcatcttca aaaatattaa cttctctttc tatttttatt tttctgttgt
    11761 atatcttatt gatagattct ttgtcgagtt ttatgtccct atacatatca gggcaatagc
    11821 aacaaacata atttcttttt tttcgacgtt taaatttcac aactttctct atgctaattt
    11881 ctaatttttt tgtaccatct tttataaatt taatatcttc tatgttctcg cttgaaaaaa
    11941 tttctgtcaa tcttaaagta tctacagttt ctctaattat ttgcttttta taagaagaga
    12001 atccatatcc aaaagaaagg tttaatttat tagggatgta gccattttta tatttaaaca
    12061 tcttaatttt ttccaattca gcattatctt taaatttcaa cttttcatac ctaaaagtta
    12121 ctttgctaaa aagagtatat tctacatcaa tatattcttt tgcaatctta aatttatcgc
    12181 taccttcaat cacaatcata gcctatctcc aatcgtttta tattaaaata aggccataga
    12241 acatggtcta atagtactca ttagcactat aagacaatgt taacacaaaa agacaaaaaa
    12301 aggttattta tttcaaataa cctttttttt aataaaaaaa catactaaaa agttgattat
    12361 ccgctaaaac acagtatcta tcagcatttt caagaaaaca aacacatcaa tttactacta
    12421 atttactacc aatgaatgag ccttgatacg ctagttttcc tagatatgca aagatatggt
    12481 acggcacatc agtattgaag caacaaaaaa attgaatatt tcatagacta tggaacgcct
    12541 aaaatgacgt tccttttcta tttccagccg ttcttattga acggctattt tattacccaa
    12601 aatgaaagga gcatttagaa ttatgaaaaa gatattgaaa cgattgtgta cgggcgtact
    12661 tgcctttgcg actgtcgtta ctgctttacc gactacactt gttcatgcag aaagtaagca
    12721 atactggacg gaaagtgaag aacgtgttgg tatcgttgaa aaggtaatga atgacggttc
    12781 gattggttct acctttaacg aggggcattt gaccgttgaa ggcgaggacg cttactgtat
    12841 tgacattaac acagatttta agaacggcta taagaccaga gctgacgcaa gctcccgtat
    12901 gagtgccgac cagataagtg atgttgcttt atctatcgaa tatgtgaagc aatacaccgc
    12961 cagccacaat gagataagca acaatcacgc ctacctttta agacagcttg ttgtctggca
    13021 aagattgagt gttcacttag gctggcaatg tgataacgtg cgagcttctt atgatgaaat
    13081 tccaaaggct acgcaagatg aagtgtttgc tggtgcaaaa gcctttgtca aagaaaacaa
    13141 aggacgctat gaatgtggcg gttatatcta ttctggcgag gggcaggaat tagggcaatt
    13201 ctgggcgaag ttaaatgttg gaaatgccaa acttcaaaaa acttccaata ataccagcat
    13261 tacagacggt aacgggaatt actctatcgc tggtgcgact tacggtgtct tttctgataa
    13321 ggactgcaca aaacagcttg ctacccttac gaccgacaac agcggaaata cagaggttgc
    13381 agaagtcaaa gctactaccg tatatatcaa agaattgact gcaccaatgg gatttaaagt
    13441 ggataaaacc atatattcct taaaagttga agctggaaag acagcgactt tgaaagtatc
    13501 tgatacgcca aaagttgccg atactttgat tgagcttttc aagattgaca tggaaacaca
    13561 gaaagccaac ccacaaggta acgcttcttt agagggtgca gagtttacat ggaaatatta
    13621 tgcaggcttc tacactaaag acaatcttcc tgccgaagct actcgtacat gggttacaaa
    13681 gacaatcgct gaaacagaca gcaacggtac aacccactac atcacaaaat tagcagacgc
    13741 atacaaggta tctggcgaca gtttctatat gcaggacgga aaagcggttt tacctcttgg
    13801 tacactaact tgcgaagaaa caaaatcacc aacgggctac ttattagacg gtgcatatat
    13861 gaaggcaaac ggtagtgaag aacagataaa aggcttatac cttacacaga ttaccgagga
    13921 cggcgacctt gccgtattaa ctggaagtaa ccagttttct gtatcagaca aagtcattcg
    13981 tggcggtgtc aaaattcaga aacgagattt agaaacgggc gataccaaac cacaaggaag
    14041 tgcaaccttg aaagatactg cctttgacat catttcctta aatgataatt ctgttttggt
    14101 tgagggcaag ctatacaaga aaaatgaagt cgtaaaaaca atccatacgg acattgaggg
    14161 tatcgcttct acttccgctg accttttgcc ttacggaaat ttccgtatcg ttgagaaaca
    14221 agctccaaac ggttacttga cagacggtgc gaagccgatt gattttgcaa tcacagaaaa
    14281 cggaaaaatc gtggacttaa cagacgaagc ccactctgtc tacaatcaga ttaaacgtgg
    14341 agatattgag ggcgtgaaaa tcggtgcagg cacacacaag cgtcttgctg atgttccctt
    14401 taggatcaca agcaagacaa cgggcgaaag tcatgttgtg gtaactgatg ataacgggca
    14461 attctccact tcttctgact gggcttcaca taagcacaat accaacgcag gaaagaccag
    14521 cgaggacggt gtgtggtttg gaacttctga accagacgac agcaaaggtg cgttgcctta
    14581 cgatacttac attattgaag aattacgctc tgacagcaac aaaggttttg agcttattcc
    14641 accatttgaa atcgtggtat caagaaataa tcttgtgatt gatttaggga ctttgactga
    14701 tgaatacgaa aaggaaatca ctatccatac cacagcaacc agcaaagacg gcgaaaagac
    14761 aatccttgca ggaaaagagg taacaatcgt tgatactgtc aaattagacg gacttacaaa
    14821 aggcactaag tatcagttaa aaggctggca gatgttaaag gaagaaaacg ccgagattat
    14881 cattgacggg aaacgtgtag aaagcgatta tacctttgtt gctgatgatg aagaaatgaa
    14941 agtggaaatt tcttatacat tcaatgcgtc cgagcttggt ggtaaaaatc tggtaacatt
    15001 cgaggaatta tacgacttaa gcaatccaga cgaacccgtg aaagttgcgg agcataaaga
    15061 tattgaggac gacgaacaga cggtacttat tacagaacgt atcatcaaaa tacatacaac
    15121 tgctaccgat aaggacagta acaaagagct tgaagcagga aaagacgtaa caatcattga
    15181 tactgtaacc ttggagggct tggaagttgg tacaaagtat aaacttgtgg gctggcagat
    15241 gttgaaagaa gaaaatgcag agcttcttat caatggaaaa cgtgtggaaa gtgattatac
    15301 gtttatcgct gacagcgaaa ctatgaaagt ggaagttacc tttacctttg acgctacttc
    15361 tcttgacggc aaacagcttg taacttttga agaattgtat gatttaagca atccaaacga
    15421 gccgaaaaaa gttactgagc ataaggacat tgaggacgag ggacagacga ttacttttaa
    15481 ggaaaaacca gaagtcccag agaaacccga aacgccaaca acaccagaaa aaccgagcag
    15541 accaacagac agtcctaaaa cgggcgacaa cacaaatgtc atggcatttc tggtaatgtt
    15601 gcttacatct gctggtggac tggctggaac atatctttac aaacgccgta aaatgaagaa
    15661 atcataaggc ggtaacggta tgagaaaaga aaaatcacgc tctacgccga agctgtcaaa
    15721 cggcagacgt ttacttattc tggcttgtct gccaacaacc tgcaagcaca cggatagtca
    15781 agggtgcgaa gcattgattt accctttact atctggggga ttgctggtat ttccctaatt
    15841 gccagaataa gaaagcacaa tcaacaaact taaaaaagaa gaaagaaacg aggtaaaaca
    15901 ctatggaatt aaaatatgta gtgccgaaca tggcacagac ttttggaaac ttggaatttg
    15961 ctggcgaaaa caagattgaa cagcaacgca ttaacggacg ctttacgccc gtatcaagaa
    16021 gctacaactt gtactctgac gtacaaaggg ctgatgatat tatcgtaacc attccagcga
    16081 aagcaggcga aaagcatttc aaaccagaac agcgagtaaa acttatcaat ccaaagatta
    16141 ccgcagacgg taaagcggtc aaaggacgag ggttcacaaa ctatatctta acggctgatg
    16201 atatgttgcc agccgaggaa agcaagtaag ggggtaaatg attatgagat tagcaaacgg
    16261 tattgtatta gacaaggaaa aaactttcgg tgtattgaag ttctctgccc ttagacatga
    16321 ggtgcgagca gaaaatgatg acggttcaac aagtgaagaa atcacacgaa gaacctacga
    16381 tttgaaatgt agtgtgcagg aacgcatgat acaagtgtca atccctgctg atattcccgt
    16441 caaagactat ccatataatg cagaggtaga acttgtcaat cccgtagccg atacggtagc
    16501 aaacgccagc tatcgaggtg tcaatgtgga ctggtatatc aaggctgacg atattatctt
    16561 gaagaacaaa ggcggtcaag ctggcaatcc acagaaccat ataccacagg gacaaccaaa
    16621 gaaataagct ggcaaactag attttcattg tagcggatat aagcaactgt tataatatgt
    16681 gtatgaagaa acggataaat tgaaatttgt ggaggaaaaa gataatggca aaagaaaaag
    16741 gttcattcaa agaatttcta tcggcaatcg cgccagaaca ccaagcgttt gtcgagaaac
    16801 ttaataccaa acttattgag cagggctgtg accttgtaat aaaggaagca aaaagcggtt
    16861 acaccgcttc ataccaacta gagaagaaaa cagtaatgaa ttgggtgttt cgcaagacag
    16921 gtgtttttgc acggatttac ggagacaacg ctggaaagta tgaggatata atcgcttcgc
    16981 ttcctgctga tatgcaaaag aaaatgacta cttctcgtga ctgcaagaga ctgattgacc
    17041 ctaatgcctg tagtgatacc tgtgtgaaag gttttgttta tgccttgaat ggcgacacac
    17101 ataaaaaatg ccgtaatgac ggaatgctct ttgtactgac aaatgaaaca gcggagcata
    17161 tagctgggct ggtctgtgca gaggttaatg tgcgtaagtc agctttataa cgacagaaaa
    17221 gttaagaaca ataaatccca gtttgacaac ttcatatcca tacacacaaa gcagttagtc
    17281 ttaggattga ctgctttttt cgtacccaga aaggagtgat tgatttatga aaatgatttt
    17341 aaacaaagga caccgtatca gagccagcga caagaacctt gtctaccact tttccatagg
    17401 gacgcttctg tttgtattca ttgcggttat cctgctactg aatatgaaac agctcatgcg
    17461 taccgattgg gagcatttca gcttattaga caacggtttg acgctttcca cttacaactt
    17521 cataacccta ctgatagcga ctggtgtttg tgcattggtc gcttttctgt attaccgctt
    17581 ctgttatgac agtttcaaga agctcctgca ccgtcaaaag ctggcacgaa tgatactgga
    17641 aaacaagtgg tatgaagcag acaccgtaca agacagcggt ttttttactg acctgcaaag
    17701 cagatcaagg gaaaaaatca tctggtttcc aaagatttat tatcaaatgg aaaaaggact
    17761 gcttcatatc cgctgtgaaa ttacattagg aaaatatcaa gaccagctct tacggttaga
    17821 ggataaattg gaaagtggct tgtattgtga gctgaccgac aagacgttac atgacggtta
    17881 tatcgaatac accatgcttt atgacatgat agcgaaccgc attaccattg atgaagtgaa
    17941 agcagaaaac ggctgtctta ggctgatgaa aaatcttgta tgggaatatg acgcactccc
    18001 tcacgctctg attgctggtg ggactggtgg cggtaaaacg tattttctgc tgacgcttat
    18061 tgaagctctg ttacatacga acgctgttct ttacatatta gacccgaaga acgctgacct
    18121 tgcagattta ggtacggtca tgggaaatgt gtatcacacc aaagaagaaa tgattgattg
    18181 tgtcaatgcc ttttatgagg gcatggtaca aagaagcgaa gaaatgaagc gacacccaga
    18241 ctataaaacg ggcgaaaact atgcctatct tggtttaccg ccctgctttc ttatctttga
    18301 tgaatatgtg gcgttttttg aaatgctggg gacaaaagaa agtgtgagcc tacttagcca
    18361 gctaaagaaa atcgtcatgt taggacgaca agcaggatat ttccttattg ttgcctgcca
    18421 gcgtccagac gcaaaatatt tctcggacgg tatcagagat aattttaact tccgtgttgg
    18481 cttggggcgt atcagcgaat taggttacgg tatgctgttc ggttctgatg tgaaaaagca
    18541 gtttttccag aagcgtatca agggacgtgg ctattgtgat gtgggaacaa gcgtcatcag
    18601 tgaattttac acacccttag ttccaaaagg acatgatttt ttgcagacca taggctcact
    18661 tgcatacgca aggcaggacg ggacggcgac gtgcgaagcg aaaggcgacg gcacggacta
    18721 gccgttgctg gtgtggcgtt agccacgcca gcagatacaa gcccctcatt atctaacaga
    18781 ggggcacaat tacggcggaa acttccacaa aaacctttgc aagcccgcat aaaataaggg
    18841 cttttcgtac atttcgcctt ttgtgataaa gccttgaaat tatcacaata tcacaaaatt
    18901 atcacgattg ggggtatgac aactgaatga aactgatttt ttaaccgcct tgaaagaaaa
    18961 acgctgtgca tacggcgtat cgcagacaag gcttgcaatc atggcaaata tcagccgtga
    19021 acatttgagc cgtatcgaag caggaaaggt aacgctgacc gaggacatga aacatacgct
    19081 tttagaagcg gtggaaaaat tcaatcccga caatcccatg ttcctgctgt ttgattatgt
    19141 gcgtatccgt tttcccacaa tggatataaa acatatcatc aaggatatat taaagctcaa
    19201 tatcgactat atgcttcacg aagattacgg acattacaaa tacaccgagc attaccatat
    19261 cggagatgtg tttgtgtatg tatcgcagga cgaggaaaaa ggtgtcctgc tggaactgaa
    19321 aggcaaaggc tgtcgccagt ttgaaagcta ccttgtggca caagagcgaa gctggtatga
    19381 cttcttcacg gacgcattgg ttgagggcgg tgtgatgaag cgtcttgatt tggcaatcaa
    19441 tgacagaacg ggattgctgg atataccaga gctgatacaa aagtgtgaac atgaggaatg
    19501 tatttctaaa ttccgttctt tcaaaagcta tggttctggg gaactggtaa agcataatga
    19561 aacggataaa tacgatatgg gacacacgct gtatatcggt tcattttcaa gtgaagtgta
    19621 cttctgttgt tatgaaaaga attacgaaca gtacgccaaa cttggtatac cgattgagga
    19681 agtccccata aaaaatagat ttgagatacg tttgaaaaat gaacgtgcct attatgctgt
    19741 tgtggaactg ctgacacatt atgacgcaga actaacagca ttttctatta tcaatcaata
    19801 tatccgcttc gctgataaag aaccagacaa gcgaaaaagc gactggaaaa ccaatgtacg
    19861 ctgggcttgg ttcattggcg aggggcgacc gcccgtaaag ctgacgacaa aaccagaacc
    19921 ttacacattg gagcgtacac tcaactggct acaaaggcag gttgcaccaa ccttgaagat
    19981 gttgaaacag atagacaagg acaatcagac agagtatctg gaaacgattg agaagcaggc
    20041 aaagctgacc gagcgacacc accagataat caagcaacag acagccacgg ttgaagaaat
    20101 ggttgccaac gtaaaggagt gatgaaaaat gtgggaatta ttgaaagact tattgctggt
    20161 atcatttgga ataggtatcg gcgtagtctt aatgtgcatt ttaactgtcg gcaaagaagc
    20221 tgacagatgt atggaagaaa tcaaagaaag agaggacaat taaatgaatt tcggacaaaa
    20281 tttatgtgcg actcgttcct tagtgaaaag ctaaggcact aataaaagaa cgaaaaagtt
    20341 tgattattag gagaactgat aatctgacag gtggaatgat gaagtaacgc tctgaaacgc
    20401 ctgccctgag cctccgacaa gcattgaatg aggaaactca gaatgtttga agctcggtaa
    20461 agacggctga aagataccgt aatagttgat gaacatatat cttctatcga aaactatcca
    20521 gaggtgggta gcgtatccaa caggtcgggg gtctataaaa tatctatggt tagaatgttc
    20581 cacaagggac tgacgaaaca gcgaatgtac gggtctaaat ttgtacttgg tagaaatgca
    20641 aaggtacgag aaatcgtagc tggtgtggca aagtaagaat ataggttatg aaatgccata
    20701 ta
//
LOCUS       DAEMIK010000071        20611 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEMIK010000071 DAEMIK010000000
VERSION     DAEMIK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.071"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..2324
                     /locus_tag="KPK87_002139"
     CDS             105..2324
                     /locus_tag="KPK87_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2538286.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            2433..3263
                     /locus_tag="KPK87_002140"
     CDS             2433..3263
                     /locus_tag="KPK87_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG2538287.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            3253..6720
                     /gene="addB"
                     /locus_tag="KPK87_002141"
     CDS             3253..6720
                     /gene="addB"
                     /locus_tag="KPK87_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG2538288.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKNTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDMNINPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            6720..10547
                     /gene="addA"
                     /locus_tag="KPK87_002142"
     CDS             6720..10547
                     /gene="addA"
                     /locus_tag="KPK87_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG2538289.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKEDEIEEEEK
                     LDNIQLEARMVGKIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     MFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            10593..11804
                     /locus_tag="KPK87_002143"
     CDS             10593..11804
                     /locus_tag="KPK87_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG2538290.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            11791..15318
                     /locus_tag="KPK87_002144"
     CDS             11791..15318
                     /locus_tag="KPK87_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2538291.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQTIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            15449..16702
                     /locus_tag="KPK87_002145"
     CDS             15449..16702
                     /locus_tag="KPK87_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG2538292.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            16851..17903
                     /gene="ytvI"
                     /locus_tag="KPK87_002146"
     CDS             16851..17903
                     /gene="ytvI"
                     /locus_tag="KPK87_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2538293.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMESFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            17994..19220
                     /gene="pepT"
                     /locus_tag="KPK87_002147"
     CDS             17994..19220
                     /gene="pepT"
                     /locus_tag="KPK87_002147"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG2538294.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            19427..20479
                     /locus_tag="KPK87_002148"
     CDS             19427..20479
                     /locus_tag="KPK87_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG2538295.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         8777 a         1938 c         3742 g         6154 t
ORIGIN      
        1 gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt
       61 atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt
      121 atagagaaga ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag
      181 taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta ttacttgaac
      241 agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt
      301 ttaaaggtag aataagaaag attcttgaag agaataaaat agatgatact aattcatatg
      361 aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa
      421 tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata ttaaatgaat
      481 gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc
      541 aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt ttttttgaat
      601 ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat aaacttgaag
      661 aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt
      721 atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta attttagcag
      781 aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata
      841 tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat
      901 ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat
      961 tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata
     1021 ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca
     1081 agtatgttac aaaggaattt agacagaaag aatgtagaca tgctcttgaa gatatgcaaa
     1141 taaaaactgt gccaaatgga atgggatata aagaaaaccc taatccagaa cattataata
     1201 taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatacgtta
     1261 agggtataaa aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac
     1321 aagtgacaca agttgctaaa gaacttagag agaataatct aacctttgaa gaattaggac
     1381 caaattcttt gaataaaagg aaggttataa acaacatagc atatattatt gattttatgt
     1441 tgcattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg
     1501 ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct accgaagaaa
     1561 ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa
     1621 gtgatatata cttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc
     1681 caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag
     1741 aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta tcagaaaata
     1801 taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac agggtgttta
     1861 atcatataat agatgtggta tatgagataa atggatatga accagagccc ggtagtataa
     1921 cactatgtaa ctatcataag tcaaagggga tggaatggga ttgtgtgttt ttactaggtc
     1981 ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc
     2041 taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa
     2101 aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga
     2161 ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg
     2221 cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt
     2281 taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt
     2341 aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac
     2401 taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt
     2461 atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa tataaatata
     2521 ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa
     2581 aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa
     2641 tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa
     2701 aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga
     2761 cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata
     2821 tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata
     2881 gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa
     2941 tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac
     3001 caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt
     3061 atataagtat gataaaaaac actaattatg aagaacatac atacgaagac aatctataca
     3121 atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt
     3181 gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg
     3241 aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc
     3301 tatatactag atgagattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt
     3361 ttagttccag aacagtacac atttgaggct gaaaatagag taagtaagct attcttagga
     3421 aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata
     3481 gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg
     3541 atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa
     3601 tcgcagtctg gttttgtaag ctcaattaca gatatgatat cagaaatgaa acaatacaat
     3661 ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta
     3721 aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat
     3781 gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta
     3841 gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc
     3901 ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc
     3961 aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac ttattcaaaa
     4021 ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca
     4081 ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag attttataat
     4141 gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac
     4201 aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag
     4261 aatgtaagat atagagatat aaccattgct acaagagatt taaatagata tgatttttta
     4321 gtacactcta tatttaatga atataatata ccaaacttta tagataagaa gagagaggca
     4381 aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa tagacgatat
     4441 gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat
     4501 ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat
     4561 gaaaaatggg attatagaat aacccaaagt ctttcaggtc aagaaagtga atttgaactg
     4621 gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta
     4681 caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt
     4741 ttattagata taaatatgcc agaaactata gaaagtttaa tagtaaattt taaggataaa
     4801 ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta
     4861 gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta
     4921 ataaccttag gttttgatga atatgaatta gggctggttc ctcctagtat agaccaggta
     4981 cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga
     5041 actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag tgataatgat
     5101 agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt
     5161 gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca
     5221 tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt
     5281 aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaaacact
     5341 gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac
     5401 gtaataaaaa attatgacag tgatggttac aatacagaag agattaatag tatttggtta
     5461 gatatatata ggtactattt aaaagatgaa atctatagtt ctataacaaa aaaagttata
     5521 aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg
     5581 tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct
     5641 tattttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca
     5701 gatttaggaa cttttattca taatatttta gataggtttt caaaagaact tttacaagat
     5761 aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt
     5821 gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag gtataaatac
     5881 cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata
     5941 aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcgacaa tggaaaatat
     6001 ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat
     6061 agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca
     6121 ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg
     6181 tatttagatg caatccttga aagtgcaaag gatgaagata tgaatataaa tccagctgca
     6241 atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa
     6301 gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct
     6361 cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa ctctttagta
     6421 ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat
     6481 gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt atgtgaagaa
     6541 atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac
     6601 ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa caagtataaa
     6661 aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag
     6721 tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga gaatgcaatt
     6781 tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag cgtataattc
     6841 aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt gttacattta
     6901 ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag
     6961 atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta
     7021 ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact
     7081 tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa
     7141 tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg
     7201 aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt
     7261 catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta
     7321 atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg
     7381 taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat
     7441 caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa aaaattgctg
     7501 acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt
     7561 cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa
     7621 aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct ttagaaagta
     7681 tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata aaatatcttt
     7741 ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata
     7801 agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac
     7861 ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt ggatatagaa
     7921 acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac
     7981 tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac
     8041 agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact
     8101 ataatgataa aaagggaagc tcccatagaa aaataatgct ttacaaaaac tttagaagta
     8161 gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag
     8221 gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt aatgtggata
     8281 cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata
     8341 aacttgatga tgatattata aatgataaag acgatagaat taataacaaa gaagatgaaa
     8401 tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaagatta
     8461 tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa
     8521 tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg
     8581 catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaaca tatgctgatg
     8641 ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa
     8701 tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat
     8761 ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg
     8821 ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa aatgagtctg
     8881 agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg
     8941 aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa
     9001 ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga caagctaatt
     9061 taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatgttca
     9121 actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa
     9181 cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag
     9241 aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa
     9301 agagtatttt atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa
     9361 ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt
     9421 tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta
     9481 taactggttc tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa
     9541 gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa
     9601 tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta ggattagacg
     9661 cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata
     9721 ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa
     9781 tagatgtaga taatcctgac agtgattatt acagtgaaat agaagaaaaa ttaaactata
     9841 tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa
     9901 agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat
     9961 taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt ggaacagaaa
    10021 gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta agtagtgtaa
    10081 atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag
    10141 ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa
    10201 tgttgaatgc tgaaattata aatagagaaa aatccattta tgcacaagta aatatgaaag
    10261 acatatatat ttatgaaaag cttataaaca atgatgataa aaaactttat gataatgaaa
    10321 gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg
    10381 tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata
    10441 aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag
    10501 agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa
    10561 attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc
    10621 atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg
    10681 aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat
    10741 atgatacttc taatccccca gcacaagcag aaaaactatt ttatcagact gtatcaagac
    10801 ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga
    10861 gactctctgc aataacacct ctagcacatg aacaggggat acttatatat ggttatccat
    10921 taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat
    10981 gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg
    11041 aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt
    11101 ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa
    11161 atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta
    11221 tacaattagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata
    11281 ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat gcatactatt
    11341 ctggttctcc acttcaatat agcaaggatg aaagagcgta taccaaaggg gcatacatag
    11401 tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac
    11461 ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc
    11521 aagatagaga gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg
    11581 aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat
    11641 cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag
    11701 agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag
    11761 atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg
    11821 cttaatagct atattgataa acaagttata gactttgaaa aattaattga aagaggtttg
    11881 tttgggatat ttggaactac tggaagtgga aaatctacaa tcttagatgc aataactatt
    11941 gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag
    12001 gcaattatat catacgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta
    12061 gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa
    12121 gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa
    12181 gtagcacagg ttgtaggatt aacagcaaat gattttacta ggtcagtagt tttaccacaa
    12241 gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga
    12301 atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat
    12361 atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca
    12421 gaggaagtat ataacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat
    12481 aagaataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa
    12541 gaacaattaa aattagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt
    12601 gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca
    12661 tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt tgcagtaagt
    12721 aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat
    12781 gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga
    12841 ttgcaacaaa caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag
    12901 ctaaaagaaa gtggaattaa tttaaataaa actaaagttg aattagaaaa agtaaagcag
    12961 gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt
    13021 gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag
    13081 aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata
    13141 ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat
    13201 gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct
    13261 ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca
    13321 aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata
    13381 cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat
    13441 aatagaaaga acagagatga cctagaaaaa gaattagaag aactaaagta tttaaattta
    13501 gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa
    13561 catcatgaaa atcataatat aactaaatat gatgaaaata tatcttttgt caaagaaaaa
    13621 ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct
    13681 aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa
    13741 ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga
    13801 aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa
    13861 aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata
    13921 agagagagtc tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt
    13981 ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac gattactaaa
    14041 gtctctaatc tattgtcaaa agtaaaagaa ataagggaga atgaaaaaga attagaatta
    14101 ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga
    14161 gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat
    14221 gtagaaaaga aattgagtag agataataag tataaagaag ttattagcat aacgaaaggt
    14281 gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa
    14341 gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct
    14401 gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa
    14461 tcaacactaa ataaactatt ggcagaaaat aaatttgaaa gtatatatgc agtaaaaaga
    14521 gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa
    14581 gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc
    14641 ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata
    14701 ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct
    14761 ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta
    14821 ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac
    14881 caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga
    14941 tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt taatggagga
    15001 gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta
    15061 gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt
    15121 ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat agaatccttg
    15181 gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa
    15241 aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta
    15301 aaaattgaat atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat
    15361 aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata
    15421 taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat
    15481 tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcctt
    15541 aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa
    15601 gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa
    15661 taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg
    15721 agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga
    15781 gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat tggtatctaa
    15841 acctataaaa gcaattatat ttatattaag accaatagta tggatattta atataattac
    15901 aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga
    15961 agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga
    16021 gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt
    16081 acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt
    16141 atttaaaact gaaaaattaa gtagaatgcc tgtgtatgaa gaaactatag atgatattgt
    16201 aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga
    16261 tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact
    16321 tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg
    16381 aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag atatagagga
    16441 tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga
    16501 tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga
    16561 gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga
    16621 agtaattgaa gttgataata taagattttg tatagaaagt atagaaaaaa atagaattaa
    16681 aaaaataaga atatatacat aatgtaataa tgttcttaaa tttattagaa gtgtataaat
    16741 ataaaagata tcttttatat ttatacactt ctttttattt tgtattcaaa taaaactagt
    16801 atgaaatgga caaaataaaa atataaatta gaagaaagga gatttttaag atgagcatac
    16861 tagaaaataa ttttttatac aaattaaaaa acaacactat ctttttttgt atatatacat
    16921 tgttatttat ttttatttac aaagcgtttc catacatagc tccatttttt ctaggtgcta
    16981 taatagcact tatgattaat cctatatcac aaaagttaga aaataagttt cgtataaata
    17041 aaggtatctc tactcttgtt ctaagttttc ttgcagtggc tatagtcagt acagttacta
    17101 caataatagt aataaattca atgaaagaat taatgggatt tttaaataat atatctgcta
    17161 atcctgagga tataagtaat actattatgt attttttaaa taaaataaat gactttatgg
    17221 aatcatttca agaaatagcc aattttgatg tagaacaatt agtaaacaaa ttcagtggtg
    17281 aagtaatgca aattacaaaa aatcttctta ctagtatact tggattggct acttctattc
    17341 catatattat aatatttata attaccttgt ttattgctac gtactttata gcaaaagacc
    17401 ttgataagat tgaaaatagt ttttataata tgtttactgt tgatgttaga aagaaggtaa
    17461 aaaacgtaaa gaaagaggca gggctttcac ttgttggata tataagagcc tatacaatac
    17521 ttatggctat aacatttttt gcaatctggg gaagttttgc tttatttgga ttgaaatatg
    17581 gtctaatagt agggtttgta ggagctttaa tggatttaat tccattccta ggtataataa
    17641 caatatatct tccagtaatt gtttattatt tccttataaa aaattatttt atagctataa
    17701 gtatgactgt gatatttttt gttttgtctt tgattagaga aatacttgaa cctaaactag
    17761 tatcagtaaa tgttggacta aatccattag ctaccctagc agcgattttt attggaatcc
    17821 aagtaaaagg aataattgga gtaatttttt gtttgggttt ggtatgtatg catgatatac
    17881 ttaagaaggt ggatattttt taattataat aattgataga tttttttaaa aataaaattt
    17941 tattcacagg taaataaaaa aatattaatc ttattaagaa agagggtata tacgtgaaac
    18001 aaaaagtagt ggaaagattt ttaaaatatg tatcatttga tacaacatct aattctcagt
    18061 gtgagaactg tccatcttca gaaggtcaaa gagttttagc taagtatata gttgaagagc
    18121 taaaaacaat gggtgttgat gatgtgagtt tagatgaaaa tagttatata atggctactt
    18181 taaaaggaaa tactgatggt gtggatacta taggattcat atcacattta gatacaattg
    18241 aagatgttag tggaaaagat attaaaccaa gaataataga aaattatgat ggaaaagaca
    18301 tagttttaaa tgaagcgtta aatgttatta cgtatgtaaa ggattctcca gagttggaag
    18361 aatttaaagg tgacgattta atagtcactg atggaacaac tctacttggg tctgatgata
    18421 aagctggtat agctgagata gtgactgcaa tagagtatct aataaatcat ccagaaatta
    18481 agcatggaga tataaaaata ggatttacac cagatgaaga aattggtaga ggtgctgatt
    18541 tatttgatgt tgaaaaattt ggagcaaaat atgcatatac tttagatgga ggaatagttg
    18601 gagaacttga atgtgaaaat tttaatgctg caaatgcaac cattactata catggtagaa
    18661 atgttcatcc aggttcagct aaaaataaaa tggtaaatgc tatacatata gctgctgaaa
    18721 tatcagaaat gttcccagca gatgaaagac cagaaacaac agaaggatat gaaggatttt
    18781 ggcatttaaa ttctataggt ggaaatgtcg agaatgtaag tatggcttat attattagag
    18841 accattgtaa agaaaaattt gaaaatagaa aatctattat gatagagaat atagaaaaaa
    18901 taaataagaa atacgataat agagtggaat tggatttaaa agactcttat tacaatatga
    18961 aagaaaagat tgagccagtt atgtttatag ttgatatagc taaagaagct atggaggaat
    19021 taggtataaa accacgttta gttcctgtaa gaggtggtac tgatggagca agattatctt
    19081 ttaatggatt accttgtcca aatatattta ctggtggatt aaacttccat gggaaaaatg
    19141 agtgtatacc agttagttct atggagaaag ctactaaact aattgtaaga atagctgaaa
    19201 aatatgctga aagagtttag ttaaaaagta tttaaaaatt aagcatttaa agttataata
    19261 gctatatcat tctttataag tcaagctgat tataagcttg tgttgtactt aaaatggtat
    19321 agctattttt gtaattattt ttataaaaga taattatttt tagtttaggg tatcaataaa
    19381 aagtatgaat atcaataggt attaacattt aaaaggggga attgtattga aaattaaatc
    19441 taaaaaaata atagcatttg cactttcact tgggattatg ttgccaagtt gtgtatatgc
    19501 agacactatt agtaaaaatc aaaatgcaca ggtagatatg aacaattatc ttcaaggtag
    19561 aaaatctcca aaggaggaaa aacttattgg tgaagataga tttgatacag cgataaaaat
    19621 atctcaaagt ggttggaata atggttcaga aagggtgttt ctagttaact caaattcatt
    19681 gccagatgca ttggcttcaa ctcctttggc aagtaaattg gatgcaccaa tactgcttac
    19741 aaataaaaat agtataccat ataatgtaac agaggaaata aaaaggctta atccagcaga
    19801 agttatttta attggaagtg aaggagctat ttcatcaagt gtaaaagaat ctcttgaaga
    19861 tatggaagta ttagtcagta gaatcggtgg agcagataga gaagaaacat ctttacttct
    19921 tactagacag ctagatgata ctggtgattt aggtgtctca aaagtggcag tggttaatgg
    19981 gtataatgga ctggcagatg caacttcaat atcaagccca gctgcaagtg ataatacagc
    20041 tataatatat acaggtaagg atagcataag aagtgaggca aagaatttta ttacacaaaa
    20101 ctcaactaag acatacatag taggtgggga gtacagtata agtaaaaaac ttgaaggaca
    20161 gttagtaaac tcagaaagac tagcagggac agatagaaaa gatactaatg cgaaagtttt
    20221 agaaaagttt tatggaaaat caagtaaggt taataatatg tattttgcaa aagatggaag
    20281 tggtagagaa gctgatttag ttgatgggct tgctgctgga gtattagcat caaaaacaaa
    20341 gtcacctgta gtacttgcaa gtggaagttt aagtagtgca cagaaaagtt ttataaaaaa
    20401 agtaaaggct gataaatttg tacaagttgg tggaggtaaa aatagtaaac catatgcaga
    20461 agcattagcc ttacagtaat attgtgtaaa tcaaaattaa tatataataa tagcaagtaa
    20521 accaagttgc actttaatat atataataaa ataagtaaac taagttatat ttcagtataa
    20581 tatataataa aataagtaaa ctaagttata t
//
LOCUS       DAEMIK010000072        19874 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEMIK010000072 DAEMIK010000000
VERSION     DAEMIK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.072"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            110..463
                     /locus_tag="KPK87_002149"
     CDS             110..463
                     /locus_tag="KPK87_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538296.1"
                     /translation="MAKRPVEKYDFKAFGEAIKAAQTGRKESRKQVSDEMYISPRYLA
                     NIENKGQHPSLQIFFELMLRYNISVDQFLLDTPAPKDTKRRQLDALLDNMSDNGIRIV
                     TATAKEISEVEKEDK"
     gene            712..1041
                     /gene="mobC"
                     /locus_tag="KPK87_002150"
     CDS             712..1041
                     /gene="mobC"
                     /locus_tag="KPK87_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG2538297.1"
                     /translation="MNGRKRKIQIKFYVTEEERALIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIAKRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            1002..2351
                     /locus_tag="KPK87_002151"
     CDS             1002..2351
                     /locus_tag="KPK87_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG2538298.1"
                     /translation="MAVTKIKPIKSTLSKALDYIQNPDKTDGKMLVSSFGCSYETADI
                     EFGFTLAQAIEKGNNLAHHLIQSFEPGEVDYEKAHEIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGASWKGKLKITVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIEGKRTKAAKAPKEQRGVSLLIDIENSIKAAQSRGYE
                     QWAKIHNLKLAAKTMNFLTEHKIEQYADLVSRIEEVNAENEKTADALKSVEKRLANMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKQESSLILHEAAAKALKTAGVSKLPNVA
                     ALQVDYEKLREQKEALYADYGKLKKQVKEYDVIKQNIDSILRQPREPEREKGKERGE"
     gene            2444..2674
                     /locus_tag="KPK87_002152"
     CDS             2444..2674
                     /locus_tag="KPK87_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538299.1"
                     /translation="MEQEMPDYETICAAVVGEKWALEKVLDCYGDEINRLAMVKKCQP
                     DGTIKEEIDEDLRQTLIMKLLEAIPQFPMEKE"
     gene            3058..3477
                     /locus_tag="KPK87_002153"
     CDS             3058..3477
                     /locus_tag="KPK87_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2538300.1"
                     /translation="MNERERIIHLWFDMWLKQQDLGIDSIFTEDVIYTESWSPKYESR
                     KTVKHWFDEWNTRGKVLVWDIKQFFHKENQTIVEWYFKNKMNNGNIEEFDGISLIVWT
                     ENNKIMELKEFGCNLNTYNPYAQNDMPQFRDEKTKWF"
     gene            complement(3655..4020)
                     /locus_tag="KPK87_002154"
     CDS             complement(3655..4020)
                     /locus_tag="KPK87_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBG2538301.1"
                     /translation="MTGLKRLTPPQSRKVNSLVKKECCNCDNGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECGASFASTSNNVKYCPDCRKR
                     ITRRQAAERMRKRRALVTQ"
     gene            complement(4017..4223)
                     /locus_tag="KPK87_002155"
     CDS             complement(4017..4223)
                     /locus_tag="KPK87_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBG2538302.1"
                     /translation="MIRLTVEETNLLSIYNEGGKRGLMENINAALPFMDEDMRELAKR
                     TLAKIAPLTENEYAELAIFAADEV"
     gene            complement(4226..8257)
                     /locus_tag="KPK87_002156"
     CDS             complement(4226..8257)
                     /locus_tag="KPK87_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2538303.1"
                     /translation="MASLRDTVKDYQEELRDGIAWVAFWKTGRSWNAEHFSLEMGDIL
                     YPEDRSRMEEIKQADPAAVVVNGYYSGYLGEDMSLDELTAGVRRHYENGYSNIGEFIE
                     AHDNRLPPELIEEARAAAHAAGLPFSEKAYRDGEEPDPYIFDGSMSMEDYELMHRMIE
                     NERSERMEETILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEAFDRIGIDFKNYGEW
                     YFTEFQSSVPGLTTHLNEFSHPDELNYLSELLKMQFDDDREKFVAAMEYGDHTRDLQD
                     IINLAQNLDCYWLYPSIQCEEDYGHYLIEELDELELPEEAKKYFMYEEYGRDAAINDG
                     GSFTEQGYIYNNRNTFTEWYDGCEVPEEYRVTPQPPVQEKEQADLDTSVAIQTTATEQ
                     PPVLPIILSSEKPADKMKEITDRLEQGILGLYESDRYADYLRTMSKFHDYSLNNTILI
                     AMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFTVKKQVEKIDPDTQKPVFDKD
                     GKPVTEEREIKIPAFRVVSVFDISQTEGKELPALTYELTGNVEQYKDFFAALEKTFPF
                     AMGFEALSGGVKGRCNYEEKRIIINEGMDELQNIKTAIHEIAHATLHDTALAMPERPD
                     RRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRSTAASLI
                     DTIDGHFAEIQKTQNKEQTTEQAQPAQETAKQPETEIAAPELPEEKAPAKEQDGQPEQ
                     TTQTAAPYYTINEAAAKRAKDMNSFSDYKQGSATAEYRHYVDEAVQIAERQKQRVDPM
                     YHEKIDSLLDTYARKLAANMNKGYEIDARVPSILIAGGSNFPTRKKEKQNAARDSNYR
                     EWQDIQGLLDKIRSTGMGGISADDPQAVQKLEKKLESLEKSQETMKAVNAYYRKHKTL
                     DGCPHLPPEELEKLKADMASSWHLEDKPFATWALSNNSAEIRRVKDRIKSLSQQKEIG
                     FVGWEFDGGKVEANTEANRLQIFFEDKPDETTREALKSNGFRWSPKAGAWQRQLTSNA
                     YYAADYVKAIAPLTGEKPTELQWAHIRAQKVAAQEQPAQEQPEQEAPQDKDTFSIYQI
                     KGEEETRDLRFEPYDRLTATGHRVDPKNYALVYSAELTPGTSLEDIYTRFNIDHPKDF
                     KGHSLSVSDVVVLHQNGQDTAHYVDSFGYKEVLEFFKEQEKALIPDDLETGETIKTPR
                     GTFYVTAMSREQMEAAGYGLHHQSDDGKYLIMGNGTRAFAVPVQPENYLKAAEQTTEQ
                     NYNMIDGQINNTPTTAELEAKVKAGETISLVDLAEAVKADKERGKAAKTEKKPSIRAQ
                     LRADKEKTAQKKAAKSKNHELEV"
     gene            complement(8390..10480)
                     /locus_tag="KPK87_002157"
     CDS             complement(8390..10480)
                     /locus_tag="KPK87_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG2538304.1"
                     /translation="MILVIAEKPSVAQTIAAVLGVKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWNYDSLPILPQEWKYTVAADKEKQFKTLKELMHRADVSEVVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGKEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASERISNKAEADALKTTCEASKAVCTSLLKEKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGIIKLLCGKFPFMAGKD
                     FTPELAKVLNSKKVSDHHAIIPTMELAKTDLAALPESERNILTLAGARLIMATAAPHT
                     FEAVTAVFECAGQSFTARGKTVLSDGWKEIDRKYRAILKSKPETDDADSDTEKTLPPF
                     TEGQNFDNPAAKVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADP
                     AAFMKGIEDMARELVKTYPFLSEADKGRFKEEKPELGKCPRCGSPVYEGKKNYYCSNK
                     ECSFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIALADTG
                     EKYVNYRIELSKKK"
     gene            complement(10497..10685)
                     /locus_tag="KPK87_002158"
     CDS             complement(10497..10685)
                     /locus_tag="KPK87_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538305.1"
                     /translation="MTLFTDNPFEKMMIQKPNGRRDNAPPVPYPPACASCPYKGQSPC
                     VGYCLKQVQKKKETEPER"
     gene            complement(10682..11410)
                     /locus_tag="KPK87_002159"
     CDS             complement(10682..11410)
                     /locus_tag="KPK87_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG2538306.1"
                     /translation="MKNKLKKTMTALCAALILIGGFSVPAYAQGAATEQPAGDATNDS
                     NVVVEEKEETPPLTPKGNATLVDDYGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEAAKKPEICNCTEKCEAGKVNTSCLVCVTSMTSCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFMKPKQNVKGDTDLEDFEFED
                     YDEDEPEADTGETPEDEETEEETL"
     gene            complement(11400..11669)
                     /locus_tag="KPK87_002160"
     CDS             complement(11400..11669)
                     /locus_tag="KPK87_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBG2538307.1"
                     /translation="MANNKIERINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAPATETADYEEQEENADEE"
     gene            complement(11701..13680)
                     /locus_tag="KPK87_002161"
     CDS             complement(11701..13680)
                     /locus_tag="KPK87_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBG2538308.1"
                     /translation="MKQLKPRDKITQKMTRDGVVEVNETQQTAERISSREADGDFSQP
                     DSAAAERVIEHLDAAHTRKANKKAVKKEQEATALRTSTSRLQFTDEERATPELEPYIK
                     KSEKAADKLDAAKAAIPKQKKLVKERTFEEATGKAKTRLHFEEQEKPIPGGKKGNPLS
                     RPAQEAGIFVHNKIHSVEKDNSGVEGAHKSEELAERGAKYGARKLKQGYRSHKLKPYR
                     EAAKAEKAAFKANVNFQYHKTLHDNPQLTSNPLSRFMQKQQIKCQYAKAAKKGGAKAA
                     AEATRKTAKKTAEETRKAAAFAARHPAGVLIAVAALLLFIMISVGLSSCGAMFSGLTN
                     GVLGTSYTSEDSDLVAVENNYAALENGLQSEIDAIESTHPGYDEYRYDLDSIGHNPHE
                     LASYLTALLQSYTPQSAQAELERIFEKQYTLTLTEEVEIRYRTETSTDPETGETTTEE
                     VPYEYYILNVKLENKPISSFVSELLTPQQLEMFHVYLETSGNKPLIFGGGSPDGSPSE
                     DLSGVEFVNGTRPGNQELMELAKQQVGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQA
                     GKSEPRFAGCEWQGVPWFQSRGQWGARGYENIAPGDAIFFDWDLDGVADHVGLVLGRD
                     GSRVYTVEGNSGDACKIKSYDLNYQCIKGYGLMNW"
     gene            complement(13668..14627)
                     /locus_tag="KPK87_002162"
     CDS             complement(13668..14627)
                     /locus_tag="KPK87_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBG2538309.1"
                     /translation="MKTDVIINRDALAALKELPSESVHCCVTSPPYYALRDYGLDAQI
                     GQEDTPKQYIDRLTAVFGELYRVLRKDGTLWLNIADTYCSTGNKGGYADPKNPKGRTG
                     QRVARNSRVTGCKQKDLIGIPWLLAFSLREQGWYLRSDIIWQKQNPMPESCKDRPTRC
                     YEHIFLLSKEKKYYYDAAAIAEPIAPTTAERYRRARSTNSKYTQEISGQGKVQGLNRP
                     RDGGYYDDALMPTTRNKRDVWLINTVPYKGAHFAAFPPKLAETCILAGCPKGGIVIDP
                     FFGSGTTGFAAKSLDRHYIGIELNAEYCALARARIGGAGLSSN"
     gene            complement(14624..17053)
                     /locus_tag="KPK87_002163"
     CDS             complement(14624..17053)
                     /locus_tag="KPK87_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538310.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAEAIRKAKGDGKARTAQ
                     QTIPYLAMYPDGICKVTEKRYSKCVMFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     SVQLSFINQGTQREQAEKAISIPAQEDAFNSIRTEYSDMLKNQLSKGNNGLVKHKYIT
                     FTVEADNKAAAKSRLSRIETDVLNNFKVLGVTARPLSGYERLKVLHGVFHPEGEPFSF
                     SFDWLTPSGLTTKDFIAPSSFHFGEGRYFRMGKKIGAASFLEILAPELNDRMLADILD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GSEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCRLTRLDY
                     QQEAGLLSSVPIGENLIPIQRGLTTSSTAIFIPFITQELFQTGQALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREMTNAFLITDDDIIICDPEAEYFSLVQRLN
                     GQVIRLSPTGRGIDGKPQYVNPMDINLNYSEDDNPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRPFLADPDPAKMPILGDLYDELLRQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELNNRLVCFDIKQLGKQLKKLGMLIVQDQTWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNVKDLLASREVENIFENSDFVL
                     MLNQAQGDRTILAKQLNISPQQMKYVIHTEAGEGLIFYGNVVLPFVDRFPKDTELYRV
                     MTTKPEEVSSL"
     gene            complement(17001..17405)
                     /locus_tag="KPK87_002164"
     CDS             complement(17001..17405)
                     /locus_tag="KPK87_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG2538311.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQIVCFAAALLIGLPLFFLLK
                     GSAGTNLAAMAMIVVMLPCFLLAMYEKHGQPLEVVVRNIIQTKFTRPKERPYRTENLY
                     AVLEKQRKLEKEVSAIVKRTNKKDAGSRRKQA"
     gene            complement(17427..18296)
                     /locus_tag="KPK87_002165"
     CDS             complement(17427..18296)
                     /locus_tag="KPK87_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538312.1"
                     /translation="MQSILDAINEWIKEILIGAINGNLSTMFGDVNEKVGTIANEVGQ
                     TPQGWNANIFSMIQTLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     IFKAFVAVFIVTNTFNITMAVFDMAQHIVSGAAGVIGGDTNIDVAEALAAMQEGLEDM
                     EIPELLLLVLETSLVSLCMKIMSVLITVILYGRMIEIYLYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVNDMIIADNLHSAVFSLAAYTVILCFSLF
                     KSGTLAKSIFNAH"
     gene            complement(18313..18528)
                     /locus_tag="KPK87_002166"
     CDS             complement(18313..18528)
                     /locus_tag="KPK87_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG2538313.1"
                     /translation="MEFFNSAIGVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            complement(18728..18895)
                     /locus_tag="KPK87_002167"
     CDS             complement(18728..18895)
                     /locus_tag="KPK87_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538314.1"
                     /translation="MNRRIRKKKDKKITEAINGLVSIAEWQEKQRQAAIRQFVKRCEA
                     LYEQQRKERRN"
     gene            complement(18888..>19874)
                     /locus_tag="KPK87_002168"
     CDS             complement(18888..>19874)
                     /locus_tag="KPK87_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2538315.1"
                     /translation="TTLLEMINASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYK
                     KFLLSAGKTRSSILISCGARLAPFDIKELRDLMEADEMELDTIGDRKTALFVIISDTD
                     DTFNFVVSILYTQLFNLLCDKADDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIR
                     SREISASIILQSQSQLKAIYKDNADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETID
                     SFNTSENRGREVSHGLNYQKLGKQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITK
                     HPNYKYLSDYDKKNTFDMEKHLRRRPALVKPDEVFDYYEISESDLQEDTDHE"
BASE COUNT         4547 a         4845 c         4904 g         5578 t
ORIGIN      
        1 acgaacaata gcaagtatat aaatatgaat tattatggct atttagtgga actgtataaa
       61 catatccaag gagaaagggg aacagtaaaa tgtaactggg tgaataaata tggcaaaaag
      121 accggtagaa aaatatgact tcaaggcatt tggagaagct ataaaagctg cgcagacggg
      181 gcgcaaagaa agccgcaaac aggtaagtga tgaaatgtat atttctcctc gctatcttgc
      241 aaatatcgaa aacaaaggac agcaccccag cctgcaaatc ttttttgagc ttatgcttcg
      301 ttacaatata tccgtagacc agtttctttt agatacgccc gccccaaaag atacaaagcg
      361 gcggcagctc gacgctctcc ttgataatat gagcgataat ggcatacgga ttgtaacggc
      421 aactgcaaaa gaaatttcag aagtcgaaaa agaggacaaa taaatgtgtc cggcaaattg
      481 aatatgattt aggaagcgtt gtaatcttta cagattgcag cgtttttctt ttgctctttt
      541 ttggcatata gagcattgcg ttgtagtaac cagtgaggaa aaaactttcg tagcaagcat
      601 aggaaaagag taccattttc cgtattttcg ccctcccgcc ctgcggtgcg tcggttgaaa
      661 attgcttgtt gggaagtgtc ccaaaccctc aaagcggaaa ggagcgattg catgaacgga
      721 cgcaaaagaa aaatacaaat caaattctat gtaacagagg aagaaagggc attgattgag
      781 cagaaaatga agctcgtccc cacccggaac atggcggcgt atcttcgcaa gattgccatt
      841 gacgggtata tcattcagac agaccatagc gacataaaag ccatgacagc ggagatacag
      901 aaaatcgggg tcaatatcaa tcagattgca aagcgtgtca acgcgacagg cagcgtctac
      961 caagaggaca tagaggaaat aaagggggtg cttgcggaga tatggcggtt acaaagatta
     1021 agcctattaa aagcacgttg agcaaagccc ttgactatat ccaaaacccg gataaaacag
     1081 acgggaaaat gcttgtgtcc tcttttgggt gcagctatga aacggcagat attgagtttg
     1141 gatttacgct tgcacaagcc atagagaaag ggaacaatct cgcccaccat ttgatacagt
     1201 cctttgagcc gggggaagtg gattatgaga aagcccacga aatcgggaaa cagcttgccg
     1261 acgcggtaac aaagggacag catgaatacg tcttaactac ccacatagac aaagggcata
     1321 tccataacca tatcattttt tgtgcggtca attttgttga ctaccacaaa tacaattcca
     1381 ataaacgcag ctactatggc atacggaaca tgagcgataa gctgtgccgg gaaaacgggc
     1441 tgtccgttgt cgttccgggt aagggcagca agggaaaaag ctatgcagaa tatcaagcgg
     1501 aaaaggtcgg tgcaagttgg aaaggcaagc tgaaaattac cgttgatacg cttatccccc
     1561 aagtggcaag ttttgaagaa ttgttgcagc ggttacaggc agcgggctat gagataaagc
     1621 cgggaaaata tatctcctgc cgcgctccgg gacaggaacg gtttacccgc ttaaaaaccc
     1681 tcggtgcaga ttatacagag gaagcaatca aagaacggat agagggcaag cgcacaaagg
     1741 cggcgaaagc tcccaaagag cagcgcggcg tatcgctgct tattgacatt gaaaacagta
     1801 tcaaggcagc gcagagccgg ggctatgaac agtgggcgaa aatccataat ctgaaactgg
     1861 ctgcaaagac tatgaatttc ttgacagaac ataagattga gcagtacgca gatttagtca
     1921 gccggattga ggaagtaaat gcggaaaatg aaaagacagc agacgcatta aagagcgtgg
     1981 agaaacgtct tgcaaacatg gcggtgctga tgaagcacgt taccacctac caaaagacaa
     2041 aacctgtcta tgaagcatac cgcagggcaa aggataagga cgcataccgg gcaaagcagg
     2101 aaagcagtct gatactccat gaagcagcgg caaaggcact aaaaacggcg ggcgtttcca
     2161 agctcccgaa tgtcgccgcg ctacaagtag actatgaaaa gctccgggaa cagaaagaag
     2221 ccctttacgc ggactatggc aaactgaaaa aacaggtcaa agagtacgac gttatcaaac
     2281 agaacataga cagcatttta cggcagccaa gagagccgga acgggaaaaa gggaaagaac
     2341 gcggggagta gctttgttca tacttgtgtg atataatagt tcctatcaaa gacagtagcc
     2401 acatactaaa gtgatacatt ttcagcaaag gaagtgtcag aaaatggaac aggaaatgcc
     2461 ggactatgaa acgatatgcg ctgccgttgt gggcgaaaaa tgggcgttgg aaaaggtgct
     2521 tgattgttac ggcgacgaaa tcaaccgcct tgcaatggta aaaaagtgtc agccggacgg
     2581 gactatcaaa gaggaaatcg acgaagattt acggcaaaca ctcataatga aactgttaga
     2641 agctatccca caatttccaa tggaaaagga gtgataccat gacacgcgga gaaatttgga
     2701 aagacttttt caagagaaac gttttgcccg ccgttattgc attgttttta ttcttcatgt
     2761 tcaagaacat ctttacagag gacgggtaga ccaattactt ttatgtatgg ctgtgctgcg
     2821 gtatcccctt tggcattcgc cgtatgttcg tatggcttgt accgcatgga tatgacatag
     2881 ctggaacggt gggtatcgtt gccattaact tcattttgga cggcattatc ggcgggggca
     2941 ttttaatatg gcggcttgtc gttgctacgt ggtatatccc ccttacgata taccgtttac
     3001 ttaccgcaga taagagtgca acccctcaaa tcaatataga gaaaggattt aaaagttatg
     3061 aatgaaagag aaagaattat tcatttatgg tttgatatgt ggctaaaaca acaagattta
     3121 gggattgata gtatttttac agaagatgtg atttacactg aaagttggag tcctaagtat
     3181 gaaagtcgaa aaacagtaaa acattggttt gatgaatgga atactcgtgg aaaagtctta
     3241 gtgtgggata ttaaacaatt ttttcataag gaaaatcaaa ctattgtaga atggtatttc
     3301 aaaaacaaaa tgaataatgg taatattgaa gaatttgacg gaatttcttt aatagtatgg
     3361 acggaaaata acaaaataat ggagttgaaa gaatttggtt gtaatctcaa tacctataat
     3421 ccatacgccc aaaatgatat gccacaattc agagatgaaa aaacaaagtg gttttgaagt
     3481 tttaaaagcc gggacgcaga cagtcggtaa agactatccg cgccccggct ttcttatggg
     3541 tacagcgata aaatgttacg cagtagaacg ccatttttga gggaaaaggt ataaaaccct
     3601 tgccgcgtca ttttcacacc cggaaagccc tgtaaatcaa ggcttttttt gcccctactg
     3661 cgtaacaagg gcgcgtcgtt tcctcatgcg ctccgctgcc tgtctgcggg taatacgctt
     3721 ccggcaatcc gggcagtatt tgacgttgtt agaggtggac gcaaaagaag ctccgcactc
     3781 ggtacaacgc tttttatcct cggtcttgaa aagctctgca tatagcagct tatcaagggg
     3841 aagtacggcg gtctgaaacc atttgcaaag aagcgaatag gaaataagct gcgggcagac
     3901 acattcctcc ccgtcgtcca gtaaaataca gtgtccgtta tcgcagttgc agcactcttt
     3961 ttttacaagg ctgttgactt tccggctttg gggcggcgta agccgcttta gcccggtcat
     4021 acctcatcag cggcgaaaat ggcaagctcc gcatactcat tttctgtcag aggtgcaatt
     4081 ttcgcaaggg tgcgcttcgc cagttcccgc atatcctcgt ccataaacgg cagcgcagcg
     4141 ttgatgttct ccataagccc gcgcttaccg ccctcattgt aaatgctcaa caggtttgtt
     4201 tcttcaacgg tcagtctaat catgctcata cctccaattc gtgatttttt gattttgccg
     4261 cttttttctg tgcggttttt tctttgtctg ctctaagctg cgcccggata gagggcttct
     4321 tttccgtttt tgctgccttg ccgcgttcct tgtcagcctt gacagcttcc gcaaggtcaa
     4381 caagggaaat ggtttctcct gcctttacct ttgcttccag ttctgcggta gtcggggtgt
     4441 tgtttatctg cccgtcaatc atgttgtagt tttgctctgt ggtctgctcg gcagctttca
     4501 gatagttctc cggctgaacg ggaacagcaa aagccctagt gccgtttccc ataatcagat
     4561 attttccgtc gtcggactgg tggtgcaatc catatccggc agcttccatt tgctcgcggc
     4621 tcatggcggt tacatagaaa gtccctctcg gtgttttgat tgtttcgccc gtttccaagt
     4681 cgtcgggaat gagggctttt tcctgctcct tgaaaaactc cagcacttcc ttataaccga
     4741 agctgtctac atagtgggcg gtgtcctgcc cgttctgatg aagcaccacc acgtcggaaa
     4801 cggaaaggct gtgtccctta aaatctttcg ggtggtcgat attgaagcgg gtgtaaatat
     4861 cttcaaggga agtccccggc gtaagctctg cggaatagac aagcgcatag tttttcgggt
     4921 ctacccggtg tcctgtggca gtcagacggt cgtaaggttc aaagcgcaag tcccttgttt
     4981 cttcctcgcc ttttatctga taaatggaaa aggtgtcttt gtcctgtggg gcttcctgtt
     5041 ccggctgctc ctgtgcgggt tgttcttgtg cagctacctt ttgcgcccgg atatgcgccc
     5101 actgtaactc ggtaggcttt tccccggtaa gaggtgcaat cgccttgaca taatcagccg
     5161 catagtaggc attactggta agctgccgct gccatgcccc tgcttttggc gaccaacgga
     5221 agccattact ttttaaggct tcccgcgtgg tttcgtccgg cttatcttca aagaagattt
     5281 gcagtcggtt cgcttcggtg tttgcttcca ccttaccgcc gtcaaattcc caacccacaa
     5341 aaccgatttc tttttgctgt gaaagggatt tgatacggtc tttcacgcgt cggatttctg
     5401 cgctgttgtt ggataacgcc caagtcgcaa agggcttgtc ctccaaatgc caactgctcg
     5461 ccatgtctgc tttcagtttt tccagttcct ccggcggtaa atgcgggcag ccgtcaaggg
     5521 tcttatgctt gcggtaatag gcgtttacgg ctttcatggt ttcctgtgat ttttcaaggc
     5581 tttccagttt cttttctaac ttctgtactg cctgtgggtc gtctgcgctg ataccgccca
     5641 tgccggtgct gcggatttta tcaagaagtc cttgtatgtc ctgccattcc cggtaattgc
     5701 tgtcgcgggc tgcgttctgc ttttctttct tccttgtggg gaagttagag ccgcccgcaa
     5761 tcaggataga gggaacacgc gcgtcaatct catagccttt gttcatgttc gccgccagtt
     5821 tccgggcgta ggtgtcaagc aggctgtcga ttttctcatg gtacatcggg tcaacccgtt
     5881 gtttctgcct ttctgcgatt tgtacggctt catcaacata atgcctgtat tcagccgtcg
     5941 cgctgccttg cttataatcg gaaaaactgt tcatatcctt tgcacgtttg gcggcagctt
     6001 cattgatagt gtaataaggg gcggcggtct gcgtggtctg ctccggctgt ccgtcttgct
     6061 cctttgcagg ggctttttct tcgggaagct ccggcgcggc tatttctgtt tcgggctgtt
     6121 tggcggtttc ctgtgcgggc tgcgcctgtt cggtggtctg ttccttattc tgtgtctttt
     6181 ggatttctgc aaaatgtccg tctatggtat caatcagact tgcggcggta ctgcggattg
     6241 tttcaagaga gcctttcagt tctgcaagtt cttttccgct gctccacccg gcgatatatc
     6301 caaaagaata atccgacgta tcaagcccgt aatgttggca gacagcatag gcgacgcttt
     6361 ccgcctgtac ctcgcgggtg cggcggtcgg ggcgttccgg catagcaagg gcggtatcat
     6421 gcagcgtggc gtgggcgatt tcatggattg cagtcttgat attctgcaat tcgtccatgc
     6481 cctcgttgat gatgatacgc ttttcctcat aattgcagcg tccttttacg ccgccgctta
     6541 gtgcttcaaa tcccatagca aaggggaaag tcttttcaag ggcagcgaaa aaatccttgt
     6601 attgttccac gttgcctgtc agctcatagg taagggcagg aagctccttt ccctcggtct
     6661 gtgaaatatc aaagacagat accacacgaa aagcgggtat cttgatttcc ctttcctcgg
     6721 taacaggttt cccgtcctta tcgaaaacag gcttttgggt gtccgggtcg attttttcca
     6781 cctgtttctt aacggtaaac ggggacggcg caagtatctt gatagctttt tctccgggct
     6841 ttacatggcg gtcaaattcc ttttcccatt gtttatagcc tttcacaaga ttgccgccct
     6901 gcatggcgat aaggattgta ttgttaaggc tgtaatcatg gaattttgac atggtacgca
     6961 gataatcggc gtaacggtcg ctttcataca gtcctaaaat tccttgttcc agtcggtcgg
     7021 taatctcttt cattttgtca gcgggctttt cagaagatag gataatcggg agaaccgggg
     7081 gctgctctgt ggcggtggtt tgtatggcta cggaagtgtc aaggtctgcc tgttcctttt
     7141 cctgtactgg cggctgcggc gttacccggt attcctccgg cacttcgcac ccgtcgtacc
     7201 attctgtaaa ggtgttgcgg ttgttgtaga tatagccctg ttcggtaaag ctgcccccgt
     7261 cattgatagc agcgtcccgc ccgtattcct catacatgaa atattttttt gcttcttcgg
     7321 gcaactccaa ctcgtccagt tcttcaatca gataatgccc gtaatcttcc tcacactgta
     7381 tggacggata aagccagtaa caatcaaggt tttgtgcaag gttgatgatg tcctgcaagt
     7441 cccttgtatg gtcgccgtat tccatagctg caacaaattt ttcccggtcg tcgtcaaact
     7501 gcattttcaa aagttcgctc aaatagttca gttcgtcggg gtgggaaaat tcgttaaggt
     7561 gtgttgttaa acccggaacg gaagattgaa attctgtaaa ataccattct ccatagttct
     7621 tgaagtctat cccgatacgg tcaaaggctt ctttcaaatg ctcggcagtc gtggggaatg
     7681 atacccattc gcccgcaggt ctgccctcgg tgtactttcc aagattagaa agatacccgc
     7741 ttaaaatcgt ttcttccatg cgttcactcc tttcgttttc aatcatacgg tgcataagtt
     7801 cgtagtcctc catgctcatg ctcccgtcaa aaatataggg gtcgggttct tcgccgtccc
     7861 ggtaggcttt ttcagagaaa ggcagccccg cagcgtgggc ggcggctctt gcttcctcga
     7921 taagctccgg cggcagcctg ttgtcgtggg cttcgataaa ttccccgata ttgctatatc
     7981 cgttttcgta gtggcggcgt accccggcgg tcagctcgtc aaggctcata tcctcgccaa
     8041 gatagccgga ataatagccg tttaccacaa cggcggcagg gtcagcctgc ttgatttctt
     8101 ccatgcggct tctgtcctcc gggtagagaa tatcgcccat ttcaagggaa aaatgctcgg
     8161 cgttccaaga acgtcccgtt ttccaaaacg ccacccaagc gataccgtcc cttaattctt
     8221 cttgatagtc ctttacggtg tcccgtaaac ttgccatgtg taacctcctt tcttcactgt
     8281 cttagagggt ttgggaaact tcccaacaag caaaaatccc cgccgttctc ggtagagaaa
     8341 gcagggattt tacggaaaag ggtactcttt tcctatgctt gctattcagt tatttcttct
     8401 tcgacagttc aatgcggtag ttgacatatt tctcgcctgt atcagccaat gcgatagttc
     8461 cgtcgtaagt tttgcctgtc tttggggaat agagcttctt cacatttacc ttgccggatt
     8521 ttaagagtgc agcggcaatc ttcggggtaa aggttacttt tcgttcttca aagaaacggt
     8581 catttttcca catggtaaag ctacattctt tgttgctgca atagtagttt ttctttccct
     8641 catagacagg agaaccacag cggggacatt tcccaagctc cggcttttcc tctttgaaac
     8701 gccccttgtc agcttcggaa agaaacggat aggtctttac cagttctcgc gccatatcct
     8761 cgataccctt cataaatgcg gcggggtctg ccttgccttt ggcaatctgc gtcagattgt
     8821 tttcccattc tgcggtaagc tgcgggcttg tcaaggtgtc cggcaggacg cacacaaggt
     8881 tgataccgtc ttttgtggga agtagctgct tgccttttcg ctccacaaag ccgcccttta
     8941 ccagtttttc aatgacggcg gcgcgggtgg cgggagttcc aagccctttg cgttctgcgt
     9001 ccgggtcggt gtcctcgctt ccggcgcgct ccatagcgga aaggagcgac gcttcattgt
     9061 ggggctttgg cggtgttgtg tcatgctccg ttacctttgc cgctggattg tcaaagttct
     9121 gtccctcggt aaacggcggc agggtctttt ctgtgtcgct gtctgcgtcg tccgtttcag
     9181 gcttactctt taagattgcc ctgtattttc ggtcaatctc tttccacccg tcggacagta
     9241 ccgtttttcc ccttgcggta aaggactgtc cggcacactc aaaaactgcc gtaaccgctt
     9301 caaaggtatg cggtgcggca gtcgccataa tcagacgcgc cccggcaagg gtaaggatat
     9361 tcctttcgct ttccggcagc gcggcaaggt cggtctttgc aagctccata gtcgggataa
     9421 tggcatggtg gtctgatacc tttttactgt tcagcacctt tgcaagctcc ggcgtgaagt
     9481 cttttcccgc cataaaagga aatttcccgc aaagcagctt gataatgccc gctgctgtgt
     9541 cgcccatgtc gtcagtcaga tagctgctgt ctgttctcgg ataagtaaga agtctttttt
     9601 catacagggc ttgtgcaagg tcaagggtct gctttgccgt atatccgaac aggcggtttg
     9661 cttccctctg taaagaagta aggtcaaaga gcttcggcgg ggctgcggtc tttttctctt
     9721 tcaaaaggga agtacagact gcttttgacg cttcgcaagt cgtttttaat gcgtcggctt
     9781 ctgccttatt tgaaattctt tcacttgcag cttccgcgcc ggataaggca aggcgcacat
     9841 ggtagtattt ttctttcttg aaagtggtaa tcgctgcgtc ccggtcaaca agcattttta
     9901 aggtcggggt ctgtacacgc cccacgttca aggtatgatt gtaaaggaca gagaaaaggc
     9961 gggtggcatt gataccgatt agccagtcag cctttgccct gcatagtgcg gaagcaaaga
    10021 gcgcgtcgta ttccttgccg ttcttcagac gggaaaagcc ctctttgatt gcgctgtcct
    10081 ccatagaaga aatccaaagg cggcgcatag gtttcttgca gcccgccatt tcatagacaa
    10141 agcggaaaat gagttctccc tcgcgccctg cgtcgcaggc attgacgact tcggaaacat
    10201 ctgcccggtg cataagctct tttagggttt taaattgctt ttccttgtcg gcagcaacgg
    10261 tgtatttcca ttcctgcggc aaaatcggta agctgtcata gttccacttt ttatactgtt
    10321 ccccgtaggc ggcagcttcc gcaagcccta ccaaatgtcc cacgcaccaa gaaacgatat
    10381 agccgcttcc tgtgagaaat ccgtctttct tttcctttac cccaagtacg gcggcgattg
    10441 tctgcgccac actgggtttt tctgcgataa ctaaaatcaa atataagttc ctcctttcag
    10501 cgttccggct cggtttcttt tttcttctgt acctgtttca gacagtaccc cacacaaggg
    10561 gactgtccct tgtaagggca ggaagcgcaa gccgggggat aaggaacagg cggcgcatta
    10621 tcacgccgcc cgttcggttt ctgtatcatc atcttttcaa agggattgtc ggtaaacagg
    10681 gtcatagggt ttcttcctcc gtttcttcat cttcgggagt ttccccggta tctgcttccg
    10741 gctcgtcctc gtcgtagtcc tcaaattcaa aatcttcaag gtcggtatcg ccctttacat
    10801 tctgcttcgg cttcataaac ttaaagtagt agaacgctgc gccgccacca agcagggcaa
    10861 agaggactaa ggcaagcacc acaccgggat tgccgccttt ttctttcggt tcttccggct
    10921 gctcggttgg tgttggggta ggttctttgc ctgtgcagct tgtcatactg gtaacacata
    10981 ccaggcagga cgtgtttacc tttccggctt cgcatttctc cgtacagttg cagatttccg
    11041 gctttttggc agcttcgccg ttttcggtca gtgccataag gtcggcttcg tccacttgat
    11101 ttaggaaatg tacggtctgt tcgccctcgg cggcgcggtc aatgaggata tagaaatagt
    11161 tgccgctttt ggtggtaaca gtgataagct gcttattgcc gccgtaatcg tcaaccagtg
    11221 ttgcgtttcc ctttggggta aggggcggcg tttcttcttt ttcctccaca actacgttgc
    11281 tgtcgttggt cgcgtccccg gcgggctgct ctgtggctgc gccctgtgca taggcaggga
    11341 cagaaaaacc gcctataagg ataagggcgg cacaaagggc ggtcatggtc tttttcagtt
    11401 tattcttcat ctgcattttc ctcctgttct tcgtagtctg ccgtttcggt agcgggtgcg
    11461 gcgttcatgc cgggaatacc gccgccggac agcatagcgt taagttcctg cggggtcagt
    11521 cgcatagctc gtacaagctg tacgatttca aggttttccg cttcggtttt ctgcgcttcc
    11581 agtcctttta acttgttctg atactctgtg attttctcgc gggtctttgc gatttcctta
    11641 ttgatacgtt cgattttatt gtttgccata ggtcatttct cctttctttt tggggctgct
    11701 ttaccagttc atcagcccgt agcctttgat acattgatag ttaaggtcat agctctttat
    11761 cttgcaggcg tcgccggaat tgccctcaac ggtatagacg cggctgccgt ccctgccaag
    11821 cacaagcccc acatggtcgg caacgccgtc taaatcccaa tcgaaaaaga ttgcgtcgcc
    11881 cggtgcgata ttctcatagc cgcgtgcgcc ccattgtccc cttgactgaa accaagggac
    11941 accctgccat tcgcagcccg caaagcgggg ttcgcttttt ccggcttgat tgtagcacca
    12001 tgatacaaaa caggcgcacc attccacgcg gctgttaaag ccgtaccaag accagtaagg
    12061 gtagccgccc acatttccga cttgctgttt tgcaagctcc ataagctctt gatttccggg
    12121 gcgcgtaccg tttacaaact ccacgccgct taaatcctcg gacgggcttc cgtcgggaga
    12181 accgccgcca aaaatcagcg gtttgttgcc gcttgtttcc aagtacacat ggaacatttc
    12241 aagctgctgt ggcgttaaaa gctccgacac aaaagaggaa atcggcttgt tttccagttt
    12301 gacattcaag atgtaatact cataagggac ttcctcggtg gtggtttccc cggtttccgg
    12361 gtcggtgctt gtttcagtgc ggtagcgtat ttccacttcc tcggtcagcg tcaaggtgta
    12421 ctgtttttca aagatacgtt caagctctgc ctgtgcgctc tgcggggtgt agctctgcaa
    12481 aagggcggtc aagtaagacg caagttcatg tgggttatgc ccgatactgt caaggtcata
    12541 gcggtattca tcatagccgg ggtgggtgct ttcgatagcg tcaatctcac tttgcagccc
    12601 gttttccaat gcggcataat tgttttcaac tgccacaagg tcgctgtcct cggacgtgta
    12661 ggaagtgcca agcaccccgt tagtcaagcc cgaaaacata gccccgcaag aggacagccc
    12721 tacggaaatc ataataaaga gaagcagcgc ggcaactgca atcaaaacac ccgccgggtg
    12781 tcttgccgca aatgcggcgg ctttcctcgt ttcttctgcg gttttcttcg ctgtctttct
    12841 tgtggcttcc gctgcggctt ttgcccctcc tttctttgcc gcctttgcat actggcattt
    12901 tatctgctgt ttctgcatga aacgggaaag agggttagaa gtaagctgcg ggttgtcgtg
    12961 cagggtcttg tgatactgga agttgacgtt tgccttaaat gctgcctttt ctgcctttgc
    13021 tgcttcccgg taaggtttca gcttatggct gcggtagccc tgttttaact tccgcgcccc
    13081 gtatttcgcg ccgcgctcgg caagttcctc cgatttatgc gccccctcca cgccggaatt
    13141 gtccttttca acagaatgta tcttgttgtg gacgaaaatt cctgcttcct gtgcggggcg
    13201 ggacagcggg tttccctttt tgccgccggg tatcggtttt tcctgttctt caaagtgaag
    13261 tcgggttttt gcctttcctg tggcttcctc aaaggtacgc tccttaacca gtttcttttg
    13321 cttcgggata gctgcctttg ccgcgtccag tttatcggct gctttttctg attttttgat
    13381 gtagggttcc agttccggcg ttgcccgttc ctcgtcggta aactgcaagc gggaagtgga
    13441 agtacgcaaa gcagtagctt cctgctcttt tttgactgcc tttttgtttg cctttcgggt
    13501 atgtgctgcg tcaagatgtt ctatgacgcg ttccgctgcg gcactgtccg gctgtgaaaa
    13561 gtcgccgtct gcttcccggc tgctgatacg ctcggcggtc tgctgtgttt cgttgacttc
    13621 caccacgccg tcgcgggtca ttttctgtgt gattttatcg cgtggtttta attgcttgat
    13681 agtcctgcac ctccaatccg cgccctcgca agggcgcaat actctgcatt taattcaatc
    13741 ccgatataat ggcggtcaag gctttttgct gcaaatcctg tggttccgct tccgaaaaac
    13801 gggtctatga caatcccacc tttgggacag cctgccaaga tacaggtttc cgctaatttc
    13861 gggggaaaag ctgcaaagtg cgccccttta tatgggacgg tgttaatcag ccacacgtcc
    13921 cgcttgttcc gcgtagtcgg catgagcgcg tcgtcatagt agccgccgtc ccgcggtcgg
    13981 tttagtccct gtacctttcc ctgtccggag atttcctgtg tgtatttact gttagtgctg
    14041 cgcgctctgc gataccgttc tgcggtggtg ggggctatgg gttctgcaat agcggcagcg
    14101 tcatagtaat atttcttttc ctttgaaagc aggaagatat gttcatagca gcgggtcggg
    14161 cggtctttgc agctttccgg cataggattt tgtttctgcc atataatatc gctccgcaga
    14221 taccacccct gttcccgcaa ggaaaacgct aaaagccagg gaataccgat taagtccttt
    14281 tgcttgcagc cggtaacgcg gctgttcctt gcaacccgct gtccggtgcg cccttttggg
    14341 tttttcgggt cagcatagcc gcctttattt cctgtgctgc aataggtgtc tgcgatattc
    14401 agccataacg taccgtcctt tctaagcacc cggtaaagct cgccaaaaac agcggtcagc
    14461 ctgtcaatat actgtttcgg cgtgtcctcc tgtccgattt gtgcgtcaag cccatagtcc
    14521 cgtagcgcat agtacggtgg gcttgtaacg cagcaatgga cgctttcaga gggcagttct
    14581 tttaaggcgg caagtgcgtc cctgttgata atcacatctg ttttcatagg ctgctcactt
    14641 cctccggctt cgtcgtcatt acccggtaaa gctcggtatc tttcgggaag cggtctacaa
    14701 aaggcagcac cacatttcca taaaagataa gcccctcgcc cgcttcggtg tggataacat
    14761 acttcatctg ctgcggggaa atattaagct gctttgcaag gatagtacgg tcgccctgcg
    14821 cttgattgag cataaggaca aaatcgctgt tctcaaagat attttccacc tcgcggcttg
    14881 ccaaaaggtc tttgacgttc tgcgtaatgg ctgtgggtat gccgccccat ttacggaaac
    14941 gcttccaaat ctccacgctg taagctgccg tctgttcctc ttttaagagc aagtgaaact
    15001 cgtccatata gtagcgggtg gatttctttt ctgcccggtt tatcgttacc ctgttccatg
    15061 tctggtcttg cacaatgagc atacctaact ttttaagctg cttcccaagc tgctttatat
    15121 caaagcagac aaggcggtta ttcagttcca cgttggttct gtggttgaat acgttaaggc
    15181 tgccggaaac atacagctcc aatgccgccg cgatacgggc agcttccggc tccggctgcc
    15241 ttaacagctc gtcgtaaagg tcgcccaaga taggcatttt tgccgggtcg gggtctgcaa
    15301 ggaacgggcg gtacacattt cttacagccc tgtcaatgac ggtcttatcg acaggctgca
    15361 agccctcctt gccgccaata acaagctcgc agagggaaag gataaaatcg gactttaagg
    15421 ctaacgggtt gtcgtcctcg ctgtaattaa ggtttatatc catagggttc acatactggg
    15481 gcttgccgtc aatgcccctg cccgtagggg aaagacgtat cacttgccca ttaagacgct
    15541 gcacaaggga aaaatactcg gcttccgggt cgcagatgat aatgtcgtcg tcggtaatga
    15601 gaaaagcgtt tgtcatttcc ctttttgccg caaaggattt tccgcttccc ggcgttccca
    15661 agataagccc gtttgggttt ttgagctgct ttctgtcgca gagtatcatg ttgttagaaa
    15721 gggcgttcaa gccatagtaa agggcttgcc ccgtctgaaa aagctcctgc gtgataaagg
    15781 gaatgaaaat agcggtgctg ctcgttgtca aacctctctg aatagggata aggttttccc
    15841 caatcggcac actggataaa agccccgctt cctgctgata gtcaaggcgt gtcaggcggc
    15901 agttgtattt ctgtgcgata cccgccgccg cgaaaatgtc attttccagt ttccgcttcg
    15961 tgtctgccat gtttaccaca aggaacgtaa ggagaaacat tcgctcgttg cggctctgta
    16021 agtcctgcaa caggttcttt gcttcgctgc caaaggtggc aaggtcggac gggattatat
    16081 ccatgtcata gccgcttcta accgcttttt tctgttcctc aatcttcatc ttgtcaaggt
    16141 ctgtgatttt gcgcttaatg gtcttgattg cttccgactg gtcgatactg cggatatgta
    16201 agttgacgat tacgcctgtt tccaagtcca gtatgtcggc aagcatacgg tcgttgagtt
    16261 ccggcgcaag gatttcaagg aagctcgccg cgccgatttt cttccccatg cggaaatatc
    16321 tgccctcgcc aaaatggaaa gaggacgggg cgataaagtc ctttgtagta agccccgacg
    16381 gggtaagcca gtcaaaggaa aaggagaacg gctcgccctc cgggtggaat accccatgca
    16441 gcacctttaa gcgttcatag ccggataagg ggcgggcggt tacgccaagc accttgaaat
    16501 tgttaagtac gtcggtttcg atacgggaaa gccttgactt tgcagccgcc ttgttatcag
    16561 cttcaacggt aaaggtaatg tatttgtgct tcacaagccc gttgttgcct ttactaagct
    16621 gatttttcag catatcggaa tactcggtgc ggatagagtt gaaagcgtcc tcctgtgccg
    16681 ggatagagat agctttttct gcctgttccc gctgcgtccc ttgattgatg aaagaaagct
    16741 gcacgctcac gctcgcgtca aagtaattga ggaaatcgca ccagttttca aaaatggcgg
    16801 tcttatcgtc agcctgtgca agctgataat tgatgtcctc gaacatgacg cactttgaat
    16861 atctcttttc cgtaaccttg cagataccgt ccgggtacat ggcaaggtag ggaatggtct
    16921 gctgtgcggt gcgggctttg ccgtcgcctt ttgcttttcg gatagcttcg gcaatctgtt
    16981 tcttttcggc gcgggtcagc ttacgcctgt ttcctgcggc ttcccgcgtc ttttttgttt
    17041 gtccttttga caatcgctga tacctccttt tcaagttttc gctgcttttc caagacagcg
    17101 tataggtttt cggttctgta aggtcgctcc tttgggcggg taaactttgt ctgaatgatg
    17161 tttcttacca ccacttcaag gggctgtcca tgcttttcat acatggcaag caggaagcag
    17221 gggagcatga cgacaatcat cgccatagcc gcaaggtttg tccctgcgct gcctttgagc
    17281 aaaaaaaaca gcggcagtcc gattaagaga gccgccgcaa aacaaacgat ttgccgcttt
    17341 gtcagattga aagcgacttt tgttttgact ttggaaaggt ctttgggtac aggtacatac
    17401 gccaagaaaa aacctccttt ctgcccttag tgggcgttga atatggattt tgccagtgtg
    17461 ccggatttga acagggaaaa acagaggata accgtatagg cggcaaggga aaataccgcg
    17521 ctgtgcagat tgtctgcgat tatcatatcg tttaccagta cagcgtaaat accgacacat
    17581 atcataatga ggaagccttg aaaaccaaga gcgaacagcc cttttaggta gttgttccca
    17641 atctgtcccc attctctgtt agtcatagtt gcaaatggaa taggcgatac cgaacagtaa
    17701 aggtaaatct ctatcattct gccgtagagg ataacggtta tcagtacgga catgattttc
    17761 atgcaaaggc tcacaaggct tgtttcaagc acaagtaaga gcagttcggg gatttccata
    17821 tcttcaagcc cctcctgcat ggcggcaagg gcttcggcaa cgtcgatatt cgtatcgccg
    17881 ccgataaccc ccgctgcgcc ggaaacgata tgctgcgcca tatcaaagac tgccattgtg
    17941 atgttgaacg tgttggttac gataaacacc gcgacaaagg ctttgaatat ccacttaaag
    18001 aacatgaatg tttctatgtc gtgcatattg ttcttttccg ttaccatgct gattagctcg
    18061 tagcatagga cataggtaat gacaagcccc gcaatcggga cgattacatt ttcagaaagg
    18121 gtctgtatca tgctgaaaat gtttgcgttc caaccctgcg gggtctgtcc tacctcgttt
    18181 gcgatagtcc cgactttttc gtttacgtcc ccgaacatag ttgacagatt accgtttatc
    18241 gctccgataa ggatttcctt tatccattcg ttaatcgcgt caagtatgct ctgcataggc
    18301 agctacccgc gcttaaccga acaagccgga aagcagaggt acaaggatag tgccgataag
    18361 ggcaacgccg ccgcccgcca tgagctgttt aatgccctgt gattttgcac ccggattgtc
    18421 gtttccatat ccctctaaga gattgataac gccccaaatg ccaagtcctg ctccaagagc
    18481 gataaccaaa gtctgtaata cgccgattgc actgttgaaa aattccataa aactcctttc
    18541 tgccgcaaat gcggcttgcc ccgtaaaagg gcataaaaaa cggcggtcag ttttcaaact
    18601 gccgcggtca taagtccccg cgctgcgtaa agttctgccg cgcggcggta ttcagttgtc
    18661 agtgtgggcg ataggaattt ttgtaggggc gcgtatcatg caagttgtct ttttctttgt
    18721 tactgcgtca attcctcctt tcttttcgct gctgctcata cagggcttca caccgtttca
    18781 caaactgccg gatagcagcc tgccgctgtt tttcctgcca ctcggcaatc gaaaccagtc
    18841 cgtttatggc ttctgtgatt ttcttgtcct ttttctttct gatacgtcta ttcatggtcg
    18901 gtgtcctcct gcaaatcgct ttcgctgatt tcgtagtagt caaatacttc gtccggcttc
    18961 acaagggcgg gtctgcgcct taaatgcttc tccatatcaa aagtattttt cttgtcatag
    19021 tcggaaagat atttatagtt ggggtgcttt gtaatgtcgt acttatcaga gaagaacggg
    19081 cgcacccctc gtagctgtaa aatacatttc ccgccgtcca taaccgctat ttcatcttcc
    19141 gtcataagct gcttgcctaa cttctgatag ttcagcccat gcgatacctc gcgcccccgg
    19201 ttctcggaag tgttgaagct gtcaatggtt tctttcccca agatttccga catttctttg
    19261 agggtggttt tctccttgcc gcccaagaaa agggtggtgt cgcagttgcc gactatggta
    19321 tcggcgttgt ccttatagat tgcctttaac tggctttgcg actgtaagat aatcgacgcg
    19381 gagatttccc tgcttcgtat ggtggctatg agcttttcaa acttcggtat ctgtccgata
    19441 ttcgcaaact cgtctaacag acagcgcaca tggacgggca gcctgccgcc gtattcatca
    19501 tctgccttgt cgcaaagcag attgaaaagc tgcgtgtaaa ggattgatac gacaaagtta
    19561 aaggtatcgt cggtgtcgga aatgataaca aacagggcag tctttcggtc gcctatggtg
    19621 tcaagctcca tttcgtcggc ttccataagg tcgcgcagct ccttaatgtc aaaaggggca
    19681 agccgcgcac cgcaggaaat gaggatagag cttcgtgttt ttcccgcaga taacaagaat
    19741 ttcttatact gccggacggc aaagtgttcc gggtcttttt cctccaaccg ttcaaacatg
    19801 aggtcaacgg gggactgaaa ttccgggtcg tcctcgcggg cttcactggc atttatcatt
    19861 tcaagcagcg tcgt
//
LOCUS       DAEMIK010000073        19631 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEMIK010000073 DAEMIK010000000
VERSION     DAEMIK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.073"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1325)
                     /locus_tag="KPK87_002169"
     CDS             complement(<1..1325)
                     /locus_tag="KPK87_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG2538316.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYM"
     gene            complement(1336..2556)
                     /locus_tag="KPK87_002170"
     CDS             complement(1336..2556)
                     /locus_tag="KPK87_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG2538317.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2570..3358)
                     /locus_tag="KPK87_002171"
     CDS             complement(2570..3358)
                     /locus_tag="KPK87_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2538318.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3375..4649)
                     /gene="larA"
                     /locus_tag="KPK87_002172"
     CDS             complement(3375..4649)
                     /gene="larA"
                     /locus_tag="KPK87_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG2538319.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4767..5480)
                     /locus_tag="KPK87_002173"
     CDS             complement(4767..5480)
                     /locus_tag="KPK87_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG2538320.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5605..6864
                     /locus_tag="KPK87_002174"
     CDS             5605..6864
                     /locus_tag="KPK87_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG2538321.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7144..7710
                     /locus_tag="KPK87_002175"
     CDS             7144..7710
                     /locus_tag="KPK87_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2538322.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7911..9116)
                     /locus_tag="KPK87_002176"
     CDS             complement(7911..9116)
                     /locus_tag="KPK87_002176"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG2538323.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9219..9325)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9417..9785)
                     /locus_tag="KPK87_002177"
     CDS             complement(9417..9785)
                     /locus_tag="KPK87_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG2538324.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTENFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9837..11114)
                     /gene="obgE"
                     /locus_tag="KPK87_002178"
     CDS             complement(9837..11114)
                     /gene="obgE"
                     /locus_tag="KPK87_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG2538325.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11225..11515)
                     /gene="rpmA"
                     /locus_tag="KPK87_002179"
     CDS             complement(11225..11515)
                     /gene="rpmA"
                     /locus_tag="KPK87_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG2538326.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11529..11846)
                     /locus_tag="KPK87_002180"
     CDS             complement(11529..11846)
                     /locus_tag="KPK87_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG2538327.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVGEIKREYPKNIKI
                     LKK"
     gene            complement(11861..12172)
                     /gene="rplU"
                     /locus_tag="KPK87_002181"
     CDS             complement(11861..12172)
                     /gene="rplU"
                     /locus_tag="KPK87_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG2538328.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12273..13694)
                     /locus_tag="KPK87_002182"
     CDS             complement(12273..13694)
                     /locus_tag="KPK87_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG2538329.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13712..14413)
                     /locus_tag="KPK87_002183"
     CDS             complement(13712..14413)
                     /locus_tag="KPK87_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG2538330.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14415..16259)
                     /locus_tag="KPK87_002184"
     CDS             complement(14415..16259)
                     /locus_tag="KPK87_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG2538331.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16578..17771
                     /locus_tag="KPK87_002185"
     CDS             16578..17771
                     /locus_tag="KPK87_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG2538332.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(17983..19065)
                     /locus_tag="KPK87_002186"
     CDS             complement(17983..19065)
                     /locus_tag="KPK87_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2538333.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
BASE COUNT         6151 a         3571 c         2251 g         7658 t
ORIGIN      
        1 atcatataag catctgaatt tgattcatgt aagtactctt ttttagcata tcctatgcca
       61 tgttcaccag aaacttgacc actaagttct ctagattttt tatacatata gtcaaaagtt
      121 tcttttaatc ttattttcca tgtattgtca tccataccat cttttaaaat atatatgtgt
      181 agatttccat ctcctgcatg accaaaactt tttattctta tttttaattt gtcttgaagc
      241 tcgtgagtgt atcttataaa ctctgctatc ttatctcttg gaacaacgac atcacattca
      301 tccatttgtg tcgtagatgc ctttatagct tctaaaaatg cacctctggc tgaccatata
      361 gaatcgtttc tttcttgtgt atctgatata aatacatcta atgcaccatt ttctaaacat
      421 aaattagcta ctttttcata ttctttttct atatcttcag tgctatttcc atcaaatgta
      481 agcaaaagat aagcatctga tgtattatct ggaaactttt tacctaaaaa ttcttctgct
      541 gccaatatta catctctttc cataaattct atagccgttg gtattgattt tgattttatt
      601 attttaggaa cagtttctat tgccattgat aaatctggaa atggtataag caaacttata
      661 cttttctttg gtaaaggaag caattttaat atagctttag taactattcc taaagtacct
      721 tctgagccca ctaataaatc ctttatacta tatccagaac tatttttaac tacctttcca
      781 cctacattta taatttctcc atttggtagt acaacttcaa gacctcttac ataatccctt
      841 gtaactccat attttactgc tctcatacca cctgcattag tgtttatatt acctgcaatt
      901 gtagcacttt tttcacctgg gtcaggtggg taaaataaat ctctatcttg aacatattga
      961 cctattgtca tcaataaaac acctggttct actgtcaatg ttaaattttc ataatcaact
     1021 tcaagtattt tattcatttt acatagatta atcattattc caccatttat ggcaacagca
     1081 gcacctacaa gaccagtacc ttgacctctt ggtgtgactg gtatattatt tttatatgcg
     1141 tatttcatta ttttagatac ttgttcagtt tcaagtacat ttactaaaac ttcagggtac
     1201 ttctcaatcc ctccaagttc atcatgtgaa aaatcttcat ttatatcact tccaactaat
     1261 acattttctt ctccacaagt atctattaaa aaatctatat cttttttatc gattaattta
     1321 tacatttcta tccccctatt tagctgctga tgccatatat ttttgattat tatctttgtt
     1381 attttctatt ttttcaatca atttaggaat aatatcataa atatcaccta ttaaagctaa
     1441 atgtgctaca tcaagtatag gcgctttatc atctttattt attgctacta tatagtcagc
     1501 accctgcatc cctgcaacaa attgaactgc tccagataca ccacaagtta ttatcaattt
     1561 aggttttaca gttctaccac taagacctat ttgtttttta gcatctatcc aacctgcttc
     1621 tattacaggt cttgttcctg ccacttgagc gtttagctta tcagctaatt tatatatcat
     1681 ctccatatct tcttgttttt ttattgctct acttgccaca actatcactt cagcttcttc
     1741 cagaccaact tctttatttt tttcttttac actcagtacc ttaattttag attttagttc
     1801 ttttttttct actttacata aagttatctt tcctgtagta ttctctattt tttctggtgc
     1861 tgaaaatatt ttatatctta cagtggcaaa ttgtggtcta ttattaggtg tgtttatatg
     1921 agccattata tttccaccaa atgctggtct tatttggtct aaatcagtat ttgattgaat
     1981 atcaagtatt gtacaatctg ctgtaagacc tgttctaaat ctagcggcaa gtcttggagc
     2041 taatgaccta cctaatgtag ttccaccaac taatactatc gttggtttta ttttatctat
     2101 aaagttttct attgcttttg aatatggttc tatcctaaaa tctttaaatt catcttcatc
     2161 atatacaaat acttcatcca ccccataaga aatcaaatta ctgcacttat cttttatgtc
     2221 ttttcctaca aatacacaat acacaggttg ttttattttt gatgccatct cacgagcttt
     2281 acctataagc tcgtacgtta caggatgtat atttccatta tgatgttcaa catatactgc
     2341 aattcccctc cattcatctt tattaatttg tactttacta ctctctataa attcaaatgc
     2401 gccttttggt ccacttttga cacacaactt gcacatctta cagccagcac ttatttctac
     2461 tatacctgac ttttcttcta tagccccaaa aggacatatt tctaaaattc tctttaagtc
     2521 agttatttta tcttgattta caactatctt tgacatttat tactccccct tacaccaact
     2581 ttaattcctc taatttattt acaagtgcaa aacttagttc atctgcattt cctcttacaa
     2641 tatttttatc tgtatttggc tttggaggaa atattcttac aacttgagta ggagaaccgt
     2701 taagtccata gttattttca tctttatctt caaagtcatc taagctatat acaggtattt
     2761 ctctatcttt agtattaagt tttaatttaa atgaaggtag tcttggttga aaaatgtctt
     2821 tttctacagt tattaaacat ggatattcaa tctcacatac ttgtaaatca tttggcatat
     2881 ctatttcaac aactatactt tcatctttaa cttcaattaa ttttgtaacg ttagtaacat
     2941 gaggtatatc taaaaattca gctacttcag gtcccacctg tgctgtatca ccatcagtag
     3001 tttgttttcc acaaattatt aggtctacct ttcccatctt cttaatacct tgtgatatgg
     3061 tataggaagt agccaataca tctgctcctc caaatctcct atcacttatt aatgctcctt
     3121 catcagctcc catcataaaa ctttcttcaa ttaccttctt tgcctgagga ggacccatgc
     3181 taagtacttt tagattcgct ttcttatctt cctttattct aagagctgtt tctaaagcat
     3241 ataggtcata gggattcatt tttgaatcta ctccatctct ttttagtact cctgtagttt
     3301 catcaacttc tactgaagtc gttccaggaa cttgtttaat acatgtcaaa atttccataa
     3361 agtacactcc ttttttattt aactataact gatactccat caggtataac tactactttg
     3421 ctctccttac caactatact attcgccatt tctaaagctt ctttaaagtt aaatgcatgt
     3481 ttcatatgca ttttttttat catttcagga ttacacttat ctgttaccat tatcacagta
     3541 tatttagata atattctcgc tagtatctga aattcccatt ggtctggaat cgtatctact
     3601 ctatcgacac ttctaatttt atctaaaagt tgttttggag aatctgcatt tgctacattt
     3661 tcataaaaag attgacctcc atgaccatca ttacatgctg aaatcattat tattactcca
     3721 ccgtctttac aagtagcttc agctgctgtc attcctttaa cagattgata aatattttgg
     3781 tctagtggat atccaccatt tgtagttaca acaatatcag attttataga atttactttt
     3841 gataactctg ttacaaatct acatccttta gtatgtgctt tttcgacatc accagcaaag
     3901 gaagcaatta tttttttatc tttatctatt acaacattca atatgaatgc taattttgca
     3961 actttagctg catataccat atcctcatga ataggattat tattcaattt tccagttctt
     4021 gaattatcac tatctataaa gtcactacag tgattgtaca ttatagtctt tgcagatgcg
     4081 attcctggaa gtacgctttt tctgccacct gaaaaacctg caaagaaatg aggttctata
     4141 aaaccttctg ctatcaaaag ttctgcctca tatgctagtt tgtttatata taaatctcca
     4201 cctgaaggca atatccctac tttacacata gatttatcat cagtagatat atgcataact
     4261 atatcttcat tatctactat ttcttctccc attttataaa ttagctcttc ccttgtactt
     4321 ggcctatgaa atccagttgc cactataatc ttcacatcaa tttctggatt aacattccta
     4381 atccttctta atataattgg catagttatt ctacttggta caggtcttgt atggtcactt
     4441 gtaattatga ctacattctt tttcccctta accagttctt ccagagaatt agagcctata
     4501 ggattattca tagattcttc aactatctct tcttgagtct tatgttctat ataattatcc
     4561 gattttgact ccaaaacacc gagaaaacgt tcatcaggta tttctaaaat cattccttgt
     4621 tttgaatacg gtacttttaa ctttaccatt tttaatctcc cattataata aactataaaa
     4681 ttggtatgtg gtcataccag ttttacattt atatgatagt accattatct accatttgca
     4741 agaataattt attttataac aaacttttaa ttttcatttt ttctataatt tttacttata
     4801 aaatccatat gttcctttgc tttctcacat gcaagttcta catcattttc tacaactgca
     4861 ttgtatatag cttcatgctg tttaagcaat atattttcat taaaaggttc tgcgattatt
     4921 ttttctcttg catcttctat aaatccatca aataattttg atgctgtcat aaaaagactt
     4981 tgaattaaat aatttttact taatgttgct agtttactgt gaaatttttg gtctaataac
     5041 accagtactc gattattatc ttttttatta gtttctaccc tcatttcatc tattatatgt
     5101 ttcaattcta taacttcaga agaagttatt ctctttgaag caagttttgc aatctcaatt
     5161 tctaggatta ttctaagctc taatatatct tcaagtgtac cattattaag tttaaaaatc
     5221 atagataaag gttctattaa agttttttct atatttgtac atataaaatt tccttcaccc
     5281 tgtctgcttt ctatcactcc catagtctct aataccctaa tagcttccct tatggaagtt
     5341 ctactcacat tcatttgttc agacagttct ctttctgata gtaatttatc cccctttttt
     5401 aactctccat ttagtatttt atattgtatt tgctcaatta cttgttcata aactttttta
     5461 ctagctatac tattaaacat tttttcctcc aatatgataa tcgttgttgt aatatatatc
     5521 tattatatat cttaagccat tacattcata tatttttatt acttttaata taatatctta
     5581 taactcaaat ttacaggtga tattatgaaa aaaaataaaa ttattgcttt ttttgctcca
     5641 tctatcatag ttggagttct agttttaggg attatcctat acccaaatga agctataaaa
     5701 tctgcaagag aaggcttttc tatatggtct aatgttttag ttccatcact actaccattt
     5761 ataattggag caaacttaat tgttgattta aaaatagtgg atattatagg gtttatcatc
     5821 aatcccataa ctagatttgt atttaatgtt tcaggaaaga gcgctttagc ttttgctata
     5881 tctacagtat ctggttatcc agtaggagta agtttagctt ctgaatttag gagtaatgga
     5941 caaatttcta agtatgaagc acaaagactt gtgtcttttt gttctacatc aggaccttta
     6001 ttcataatag gttcggttgg tacaggtatg ttccaaaatt caaacttagg ttatttaatg
     6061 attctctgcc attatctagg aactatattg gttggtcttt tctttagaaa ttatggtaat
     6121 gaaaatcttc caaagacaaa attggattta aaaacaaata taaaaaatgt aatcaacatt
     6181 agaaattcaa gtgggaatgg tttttttgtt ttatttggaa atgctgtatt taatggagta
     6241 aatacattac ttacagttgg tggatttgta attgtttttt cagtagtatt taaaatacta
     6301 tctcttttta atgtaatctc attaatagca tcagttatat atttgcctct ttcattttta
     6361 ggtgttagta aagaactctg tcaagcattt gtaagtggtc tatttgaaat tactatagga
     6421 tgcaataaag tatctagtat aacaagttct ccagaaattt taagagcatc tttagctagt
     6481 tttttgatag gatttagtgg attatccatc ctagctcaat gttgtacatt tctatcaaaa
     6541 acggacatag atttaaaatt atacatacta agtaaatttg cgcatggagt tttagctgct
     6601 atctttatat ttatacttta tccaattgca aactctgctg tgttggtttc tagctttgct
     6661 gatacataca acgttatata taataattta atctggtttt attatcttag ctattatgat
     6721 acaattcttc aaattgtagt aataatttat ctaatctcag ccgtatttat tgcaaaaaaa
     6781 catatgaaaa ctttatctac aaatgggaaa ctaatgcgat ataaaaatac ttttttcact
     6841 aagaaatttt cacgaaaaaa ataaaactaa aaataaaact aataaaactt aactaaatac
     6901 ttccattttt ttattatatt tgtcataatt ttaataacct ttttaattag tttaacagca
     6961 tagttatttc atcacaattt aataatttta ttagaaatat ttttcaaaaa attctcctta
     7021 atcacatata tcacacttgt ataagtttac tgggaaattt ttccattata taatatttca
     7081 tttatattat ataataaata ccgacctaaa cttaccaaat caaactaaga ggagtggttg
     7141 aaaatggcaa caagacctat agaaatagac gaatataaaa agataatgga actattacat
     7201 actggcttta cttatagtga aaatggcgtt gaaaaaagat ttagaaaaaa tcctaaagtt
     7261 gcattggcac taatgttaga agctaattta ggtctaagaa tatcagatat tttaagatta
     7321 aagataggta attttaaagg taatatgttg gaaataaatg aaaaaaagac gggcaaatta
     7381 cagtatagac cagttaataa aaatataatt gagtccataa atgaacatgc aagaaagtat
     7441 aatcttaaat caaatgacta cttagtaaat ataaagacaa aggctattca aaaacagttg
     7501 aggataattt gtaaatactt aaatctgtat aatataagta cacatagctt tagaaagctc
     7561 tatgctacta ctcaatttga aaaaagtaac aataatttag agttggttaa agaactttta
     7621 aatcacagct ctgtagccac tacacagcga tatataaggg taactcagca agctattaat
     7681 gaagctagtg aaaatttctt tattggttaa ttataacgta tacatttttc tattatctta
     7741 ttaattcacc agatattaaa aaagagtgtc tcaaaatgaa ctttttagtt catgagacac
     7801 tcttttttta taaaatcaaa tatattttta ttatttcaac actggaaaaa aggcttatct
     7861 ctctattttg agacacccta ttaaattttc ataattcttg cataaacata ttaatctatc
     7921 ttcaataatt ttttagctga ttcactgtaa ttatttcttt ttacaatttc caaaactcta
     7981 tcaatctcat gttctgactt tgcacttaca aaaatcgcca attcttcatc atctataata
     8041 tcataactat ctacatattt atctaaacat tctaaaattc catctttatt attataccca
     8101 ccaaatctaa ctactgcact acactcatct tctataagtc cacttaaatc caatttccct
     8161 tttgaaaaag actttatatt tgaacttcta ttatctatta tatctataat atctgcataa
     8221 acagcacttc ctgttgcacc tcttccagct cccttaccta caaaagaaag ttcacctact
     8281 gaatcaccta ctaaaataac tacgttgaac tcattatcta tctttgaaag catattatta
     8341 ttgtccacta atgctggtct aacaaatcca cttacttctc ctgattcata cttacttcta
     8401 gcaaccaatt ttatcttaca atttaatttt tttgcatatt ctatatcttt ttcatctatt
     8461 gaagttattc cttctaataa taaatctttc caatatacta tttttccata agataaagta
     8521 gacagtatag ataactttct agcactatca tatcctaaaa catctgcatc tggattaact
     8581 tctgcaaatc ccaactcttg tgcctgtttt aaagcgacat catacggtaa attttcatca
     8641 tacatctttg ataatataaa attacaagtc ccatttaaaa tagcgtttat gctctttata
     8701 ttgttgcctt ccagtgattc tatcaatggt tttaaaactg gaataccacc tgccacagat
     8761 gcttcaaact tgatgctaac attatttaag ttagctaatt ctataagatc actacctttt
     8821 tcagccagta aatctttatt agctgtcact acatgtattt ttttatttaa cgctcttgtt
     8881 acataatcaa aagctggatt taatcctcca attacttcaa ctagtatatc aatatccttg
     8941 tcaaatatat cttctatatc agtagttatc ttatcaaaat acttcttata cttgtgtttt
     9001 tctttatttt taactaaaat tccaacaact tctatatctc tttttgaagt attattttct
     9061 attaaatcaa ttaatccact tcctactgtt ccatacccta atattgctat cttcactgta
     9121 acataccccc atcattttga ttatattaaa ttatttatat atataaatat accaataaaa
     9181 aaagcttgag tataaactca agccatatga cgggtttata ctcatctttc agcataaatg
     9241 ctgcaggatt tagcaccaat gtataattaa atatactggt tgccgggctt cacagggcca
     9301 gtccctctgc cactcttgat aagattgcta atttaaattt atgtttaata gtataataca
     9361 atttcttcca attgtcacta tttttttaac ttatttccct aaaagggtat aaactattat
     9421 ctaacagctc ttttagttgg tttacccttt tttgtaacat tattattttt agtctttgct
     9481 ttcgcctgtt catgtcctgg aaaaagtatt ttaggctctt caatattcct cttgtaaata
     9541 gtaaatttaa aaccaatagc ctgcacaaat tcagctctta aagcttcaca tactgcatta
     9601 gcagtttctt tagtatcaag acctgcattt tctaatatag taacttttac taattctcta
     9661 gttcttaaca taccatctaa ttgctctaag aaattctctg taacaccttc tttaccaatt
     9721 tgtgtagtag gctttaaagt atttgccaaa gatcttaaat acgccctttg ttttcctttt
     9781 aacatattct cacctctata attctataat taaattatct ataatctata tttaaattaa
     9841 ttgtaaaact caaattctaa ttcataaatt ttaaccacat ctccatcttc tattcccatt
     9901 tctctaagcc tatcaaaaac accttgactc tccatagctt tttggaaata ttgaagggat
     9961 tccatatcat caaaatttac agaatacatt attcttctaa gtgctttacc tgtaactaca
    10021 tacacaccat cttcaatatc tatacttaac tcttcttcag taccaatatc aagttctggt
    10081 ctatacattt cttcttctga aactagctcc acatcttcta cttcttttag cattttagaa
    10141 acataggcta ttacttcatc tatgccttga cgagttgctg ctgacatttt aaatactgta
    10201 tatcctctgc cttctaattc actcttaaac ttctcaaact tactctcatc ttctaatata
    10261 tcaaatttat ttgcaactac aacttgtggt ctctttgaaa gcttttcatt gtacaattta
    10321 agctcatcat ttatcttgtc aaaatcttca atagggtctc ttccctctaa tccagatata
    10381 tctacaatat gtatcaatac ctttgttctc tcaacatgtc ttaagaactc atgacctaat
    10441 cctatgcctt cagaagcacc ctctataatt ccaggtatat ctgcaagtac aaaactatct
    10501 ccaaacttag tttgaacaac acctagattt ggtgtaagtg ttgtaaaatg atagtttgca
    10561 atttttggtt tagccttagt tacaactgat agaaatgttg atttacctac atttggaaat
    10621 cctaaaagac caacatctgc tatcatttta agttcaagag ttatccatct ttcttctcca
    10681 tctgtaccag attttgcaaa agctggagct tgtcttactg catttgcaaa gtgttggtta
    10741 cctttaccac ctataccacc ttttgcaact atagctgtat ctccttcttt ttttaaatct
    10801 gctaatacaa gacctgtagc ctcatctctt attactgtac ctgctggtac ttttaatact
    10861 aaattttctc catttttacc agctcttttt cctttgcttc cgtcgccacc attttgagct
    10921 tgatattttt tctggtactt aaagtccatt agagttctaa gccccaaatc aacttcaaat
    10981 atgatgcttg ctccacgacc accatctcca ccgtctggac caccagctgg aacgtatttt
    11041 tctcttctaa atgctaccga accatttcca ccatttccag ccttaacaaa tattcttgcc
    11101 ttatctataa acaaattctt cacctactta attaaaaact tttaattagc attaccaaaa
    11161 ataccattat aaattcatca gtattataag aaaaaggagt gcctcaacac tccttttatg
    11221 tatattactc agctattgct actggatata tactaacttt ttttctcttc ttgtcttttc
    11281 tttcgaattt aacagtacca tcaactaatg caaataaagt atcatcacta ccttttccaa
    11341 cattagttcc tggatgtatt ttagttcctc tttgtctaac tattatactt ccagcagtta
    11401 ctaattgacc atcgaatctc ttaacaccta atcttttaga tatagaatct ctaccatttt
    11461 tagaagaacc tacccctttt ttagatgcta ataattgtaa attcatgttt aacatatgga
    11521 gtcaccccct acttttttaa aatttttata ttttttgggt attcccgttt aatttctcca
    11581 accgctaatt gaaagtgatt tagtaataat tgtgcgtctt ttacatgtgt atctttgact
    11641 atacactcta tatgaccttc ttctcctact actttttcaa attcaatctt ctgtaatttg
    11701 tctaaagaat ttataactgc aatagcatta gatgtaactg cagcacatac tatatcatag
    11761 cctatttcag caaaatcagc atgtcccttt aaacaaaaac cttgcaacaa gaaatcatca
    11821 ttataataat atttaacttt tatcataact taaccaataa ctaagcgttt attttttcta
    11881 caactatctt agtgtatgat tgacgatgtc cttgttttct tctgtaatct ttttttgctt
    11941 tatacttgaa aactataact ttcttagctt taccttgttc aacaacttta gcttgaacag
    12001 atgctccttc tactactgga gaacctaatt ttaattctcc atctttagag caagctaata
    12061 cttcgtttaa agtaacaaca tcacctgcgt tagcttctaa tttttcaacg aataatacat
    12121 caccttcaga aactttatat tgctttccac cagtctttac tatagcgtac attaaataca
    12181 cctcctcggc ctcaaactcg ccagtacaag gtacttcatt tgaagattta aacctttact
    12241 gtgcggcatt acaattatta attataaaat atttacattt tattgtcaac tattttatcg
    12301 aaaataacat tggttttatt gtgctttaat gtactatttg cgtttaaaga aatctttata
    12361 ttatattttt caccaatttt atcaattata tccatataat attcttttat ttgttcaaaa
    12421 ataaaatcat taaattctac cattatgttt ttgtaaacag tatgttcact tattctcata
    12481 atttcttttt caatactatc tagtatataa tgtacagatt ttattgtttg ttcaccatca
    12541 caacatggac aagacattaa ataatactta tcaatggatt ctttttctct acgcctagca
    12601 acttctacta aaccaagttt tgtcatacct aatacttcag cttttctttt atctttatta
    12661 aattcttctt ctaggatgtg taaaatctcc ttttgatgct tctttttatg catatcaata
    12721 aaatcgatta ttattatccc agctatgtct ctaattctaa gctgtcttac aatttctttt
    12781 gcagcttcaa gattagtttt ataaacggtt tcatcaagtt taccagttcc aataaactta
    12841 cccgtattta catctataac cgtaagagct tctgtttttt caattattaa ataacctcca
    12901 ctctttaacc atactttttt atttaataat ttttctattt tagattctat tctgtataaa
    12961 tcaaatacat ctctattttc ctcaagacac agtttatcaa cataagtctt atctatacct
    13021 cttaaaatag acttaagttc agagtactta tcataactat tggtaattat agattcaata
    13081 tcatcattca cattatcttt tacatattta gttgcaaaat ctaaagactt atataataat
    13141 ttcggaccta taccaagttt atattctttt aaaatgtttt catactttaa tttcaactct
    13201 tctatatctt tctttatttc atcatgagtg caaccttgag cctcagtcct tattattaat
    13261 cctagatttt ccttatttac tgccttagta atttttttta atctaaatcg ttctttttca
    13321 tcagtaattt tatttgatat tgtagttcta tcattggaag gtatatagac aatatacctt
    13381 ccagaaatac taatctcagt gtttaatttt gcgcctttag ttcctatttc ttctttattt
    13441 atctgaacca aaatatcctg accagatttt atagtacttc ccttttttaa ttgtaaataa
    13501 gcaagtttct taaatccaat atctacaaaa caagcttcta ttcctggtat aacttttttt
    13561 acgattccac gatatatatt ggatacagtc ttcttattta aattgtcttc aacaaaaagt
    13621 tctgttaatc tttcatcttc aagtactgct gttttctggg accctattag ggactcaata
    13681 actatctttt tcacaacttc acctctcgag cttacatagg agtcactaat tccccatctt
    13741 ctaatacata taaatctctt ctcaatatat caacatctaa tgggtcaata tcaagttcaa
    13801 acatctctaa tatctttggt ataagtatat tagtatttaa attagttttt gagcctgtag
    13861 ctatagtagc agtaagagta atatgttcat cttctaaatt taaaacatca aaggttctta
    13921 tcataggtct tatatctact tcaaccatct tacctttttt attctttttt gttatgatta
    13981 tttctttatt attcataaag tcaattactt ttctttttac aaactctttc gtcataggtc
    14041 ttttcaaatc tatattaaac aaatattctc catagtctat cacagaagaa agagaaggtg
    14101 tttgtctatc aatttctttt gctttgataa aatctatccc atctggaagt tgaattgata
    14161 ctttatttaa aaattcctct actgataaat catcttcttc tatttcaata tctacatact
    14221 caccttgact ttctgtccct aaagccaaag cattgccata actcatttta gggtgaggat
    14281 taaatccttg agaatgtgat aagtttattt cagctcttct aaacgctctt tgtaacaatc
    14341 tctgaaggtc aagatgtgat atatatatca tgtcgccttc ctttttaaat tttactctta
    14401 ttatcttact catattagca aagccccctt cctatatcat gtatatttat tccacaacca
    14461 ttgcagttat gtctacaatc agaagtgatt ttttcttttt tagcattttc gtcttctcta
    14521 attaagaatt ttttactaat acctacatct atatggtccc aaggaaatac ctcttcatag
    14581 tttctatttc tatgtgcata aaaatctata gaaagattat tcttttccat agcttctttc
    14641 catatatcta aattaaaatg ttctgcccat ccatcaaatt tagcacctag tctaaatgca
    14701 tcatatataa cttttcctat tcttctatcg cctctagcta ctactgcttc catcaaacta
    14761 gtctttgaat catgataatt gtattttata ttgttatttc ttaatttatt tactaaatgt
    14821 ctctgcttat taataacttc ttctgtagta tcttgaccat gccattggaa aggtgtaaat
    14881 ggctttggta caaatgtaga agtactcact gtaacactaa aacttctctt tagctttcct
    14941 ccatttactt ttctataaat atcaataact ttataagcta atttagctat accatctaaa
    15001 tcttcataag tctcagttgg taaaccaatc ataaaataca acttaacact attccacccc
    15061 atctcaaaag ctctttctgt tgcattttcc aaatcttctt ctgtaacacc tttatttata
    15121 acatctctaa gtctttgagt tcctgcttct ggagcaaaag taagtccaga ttttcttact
    15181 tgttgtattt tatcagcaat ttccattgaa aaattatcta atctaaggga tggaagcgaa
    15241 atcccaatat tattagatgc atattcatct accaaataat cagtcaactc tgataactta
    15301 ctataatcac tcgtactaag tgatgataat gatatttcat catatccagt attcttaact
    15361 aatttatcta atatttcttt aagtctttta acactctttt ctcttattgg tctgtatatc
    15421 atacctgctt gacagaatct acatcctcta gtacatcctc taaataattc tagtactatt
    15481 ctatcatgaa cagtatctat ataaggtact attagttttt ctggataatc tacagtctca
    15541 acatctttta ttatcctttt agtaggattt ttagtaatcc cttctctatt aggagttaca
    15601 ctttgaactg taccatcttc attgtacttg acatcataaa aacttggtat gtatactcct
    15661 tctatgctag atattttata taaaaaatct tctctagtcg ttttattttt cttccattct
    15721 ttatactcat ttactacttc taaattaacc tcttcacctt cacctaatat tactacatct
    15781 acaaaatctg ctaatggttc ggaattataa gcacaaggac caccaaccat tataaatggg
    15841 tcttccaaag ttctttcttc ttttaatatt ggtatattgg ctaaatctaa tatatttaag
    15901 atattagtgt atgaaagttc atattgaagt gtaaaagtga caaaatcaaa gtttgttata
    15961 ggctctcttg actctaatgc aaataatggt atactttttt ctctcattat attttccata
    16021 tcaactgcag gagaatacac tctctcacaa aatacatctt catccttatt tattacatca
    16081 tataatatat gactccctaa gtgactcata ccaacttcat ataaatctgg aaagcaatgt
    16141 gcatatctta tcaattcact attactagta tctttatgaa ttgaatttat ttcatttcca
    16201 agatatctag caggcttttc tacttttttt aagatttttt tcaagtctac tttattcatt
    16261 tattaaaatt cctccaaaca agctctttat ttttattctt atttataagt aatattatac
    16321 acgataaaat attttctatc aaaaatataa ctaaaatatt gaatttaaaa tacatattat
    16381 tcacaaaata gaatagctaa aaatatttaa gaatattttc tattaaattt ttattgaaat
    16441 acaatttttt tacaaagtat gatatattat aaacataaaa ctattgtttt acatgactta
    16501 tcatgacatc ttttgtttca ttcacctcaa aatggggtat gttacttata taatgataaa
    16561 ttttaggagg gttaaaaatg agtaaagttt ttgaagtaaa aaaagatatc tactttactg
    16621 gcgtagtaga tgaaggttta aaagtattcg acataataat ggaaactgag tttgggacaa
    16681 cttacaactc ttatttaatc aaggacgaaa aaacggtttt atttgacact gtaaaggcaa
    16741 attttaaaga tgagttttta agtaatttat cagaggttac tgatatagct aaaattgact
    16801 atgttgtaat tcatcacaca gaaccagacc atgcaggtag tttaaaatac ttattagata
    16861 taaatcctaa tatagaagtt tattgtacta aagctgctaa actatactta gatggtcaaa
    16921 taaatagacc ttttaactgt catgtaataa aagatggtga aattttaaat atcggtaaaa
    16981 gaaatcttag atttataact gctccattct tacattgggt tgatacaatg tttacttaca
    17041 ttgaggagga caagactctt ttaacttgtg atgcatttgg ttgtcacttt gcaagtgttg
    17101 atgcagaggt agtgaatagt gaagattatc taaaatcagc taaacactac tatgattgta
    17161 tagttaagcc atttgctaaa catgttttaa gtgctgtaga taaggtggta ggactaaaca
    17221 tagaatttga tactatattg acttctcatg gtccaatgct tactaaagac ccaatggctg
    17281 ctgttaaaag atatgttgag tggtctacag aagctgttaa tacaactaat caaaatcaag
    17341 tttctatatt ctacttatca gcttatagta atactttaga gatggctaaa aagataaaag
    17401 aaggacttga taaagaaggt gcaaaggctg aattatatga tttagaagat atgacattaa
    17461 cagaaatgca cgatacatta gtagtgtcaa aagtaatatt actaggctct ccaactataa
    17521 acaagactat ggttaaacct atgtgggatt tattctctgt tatagaccca atggctaacc
    17581 aaggcaagat agcaggagta tttggttcat ttggttggag tggtgaaggt ataactatgg
    17641 ctgagacttt acttaaatct atgtcattca aaatgcctgt agaatctctt aagaaaaaat
    17701 tcttccctag tgaagagaca ttaaaagagt gtatggcatt tggtgcagaa tttgcaaaat
    17761 tagttaaata gccattaatc acacataata ttatttaaaa taaatgattt tatatttaaa
    17821 aataaattaa aatggagttg tctcaaaatt aatttttcta atctaatttt aagttcaact
    17881 ccttttttat tttctaaaat gactgatata atgataaatg caccctttgt gcgagggtgc
    17941 attatttata atatgttcta tttgtacact agtatatttg tattaattca catatctagc
    18001 gtatgctacc atccctcttc cagaattaga tgccgtttca ccaatttcca ctttaataat
    18061 tacattattt tctttacact tttttgataa ttctgctgca gaaaggttct taccagtaaa
    18121 atctaaagaa aatggcttat catattcaaa aggatactca tcaggtacac catctggagc
    18181 taatgcaacc ataccatatg gtgtattcat aaaccctatt acagtaactg tatgctttga
    18241 tgggtctaaa tttgaatttt tgcttgtaaa cttaattgta tttattgatg agttgattaa
    18301 actacacgga actccctcat acatttcaat ttttggacgt agattatttc cataaacttc
    18361 atcaagtatt gccccttgtt gatctgctat catagtgtga ttcccaggtc caactggcgc
    18421 agaaggctta gctggcgtag gtttcttaac agttggcgtt ggattctgtg tctttactac
    18481 ttcgccaaca gctggatatg ctttcttgtc aaatggtaat gggtgagtaa gactcttgta
    18541 tatgatgtct gctcctgctg atttgttggc atcaatatct acatgataca aacgtccatc
    18601 ctcaaaacga ataagattcc actgatggtc atttgttgta ctatgtacat aataacattc
    18661 aattcccata gcatcacata aagctttcaa gctcctacac attccagaac aagcaccttt
    18721 tttagtaacc aatgtataag cagtattggc tccctctgct gcgttccagt taacatatgt
    18781 tgtgttgttt ttaatgtgat tataagcaac cctaatctta gcatctgtgg tcatgttctt
    18841 tgtaattttc ttgttcataa agtctgctgt tgcttgtgct accgaattaa gttgagcttg
    18901 tgataacttt ggaccatata tactgttggt tccagctaca taacttttcg gtgtctttgc
    18961 agtaacatct ttagctgtca taccataaac tgttacactc atacatgagg ttaaaaccat
    19021 cgttgcaatc aatagtttag tagctaattt cctgattttt ttcatattag cattctcctt
    19081 tataatatat tttactttca attccctacc attaattaag ttcattttag atttaaaata
    19141 tggattatca acatgtatgc atagcaattc acgtggtcat agaatattta gatttcttgg
    19201 aaaatctgtt ctatatttcc tatatagttc gtatacaatc caactcacaa acctgcattt
    19261 ttgatatcat cacaataaaa tgataataat taatatattc aaaaatgttg gtctgtaaca
    19321 ggtatatcaa ttttccaatt attggtaatg gcgagagtga ggagaattgt caataataca
    19381 tttaaaatta aaattgtata cacttcatac attctcccct aacaacatta tacagattat
    19441 aatttgtata ttcaatacat tttttggaaa gtataattat atcaaaatat gtgattatgt
    19501 atagatacaa tccactatgg agaaatataa aagaaaagag tataacaaac aataaaaaat
    19561 aagatgattc aaattttatt ttgaaacatc ttatttactc tacaaattaa gattatttgg
    19621 tgaattattt a
//
LOCUS       DAEMIK010000074        19270 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEMIK010000074 DAEMIK010000000
VERSION     DAEMIK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.074"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            250..828
                     /locus_tag="KPK87_002187"
     CDS             250..828
                     /locus_tag="KPK87_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2538334.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            867..1445
                     /locus_tag="KPK87_002188"
     CDS             867..1445
                     /locus_tag="KPK87_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2538335.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1531..2160
                     /locus_tag="KPK87_002189"
     CDS             1531..2160
                     /locus_tag="KPK87_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2538336.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2150..4909
                     /locus_tag="KPK87_002190"
     CDS             2150..4909
                     /locus_tag="KPK87_002190"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG2538337.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4996..6558
                     /locus_tag="KPK87_002191"
     CDS             4996..6558
                     /locus_tag="KPK87_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG2538338.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEDI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6559..7695
                     /locus_tag="KPK87_002192"
     CDS             6559..7695
                     /locus_tag="KPK87_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG2538339.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7759..8835
                     /locus_tag="KPK87_002193"
     CDS             7759..8835
                     /locus_tag="KPK87_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG2538340.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8813..10051
                     /locus_tag="KPK87_002194"
     CDS             8813..10051
                     /locus_tag="KPK87_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG2538341.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10105..11193
                     /locus_tag="KPK87_002195"
     CDS             10105..11193
                     /locus_tag="KPK87_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG2538342.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11221..12012
                     /locus_tag="KPK87_002196"
     CDS             11221..12012
                     /locus_tag="KPK87_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG2538343.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12127..12681)
                     /locus_tag="KPK87_002197"
     CDS             complement(12127..12681)
                     /locus_tag="KPK87_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2538344.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12877..14079
                     /locus_tag="KPK87_002198"
     CDS             12877..14079
                     /locus_tag="KPK87_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG2538345.1"
                     /translation="MKSFVSPDNIWVLWAIVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14135..15115
                     /locus_tag="KPK87_002199"
     CDS             14135..15115
                     /locus_tag="KPK87_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2538346.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15363..16316
                     /locus_tag="KPK87_002200"
     CDS             15363..16316
                     /locus_tag="KPK87_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG2538347.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16306..17262
                     /locus_tag="KPK87_002201"
     CDS             16306..17262
                     /locus_tag="KPK87_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG2538348.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17259..18014
                     /locus_tag="KPK87_002202"
     CDS             17259..18014
                     /locus_tag="KPK87_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2538349.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18106..19071
                     /locus_tag="KPK87_002203"
     CDS             18106..19071
                     /locus_tag="KPK87_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2538350.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7645 a         1952 c         3426 g         6247 t
ORIGIN      
        1 aaaataaaat atgtacttgt ttatataaat gatatataat tacatattaa acatagcttt
       61 tatacattta ataaagtatg taaaatctat gtaaaaagta agaaatataa acattttttt
      121 aattatattt taaaaatatc aagcaagttt tcttcacaaa tgaataaaaa atgttataat
      181 atgattaaag aaaaagataa ataattagca agatttgttt agctaaaatt aacaagggag
      241 cgataaatta tgggtataac attatcaaaa ggccaaaagg ttgatttaac taaaggaaat
      301 ccaggtctta aaaatatttt agtaggttta ggatgggata ctaacaagta tgatggtgga
      361 tttgactttg atttagatac agcagcattt ttaacaggag caagtggaag tgttactaat
      421 gatggagatt ttgtatttta caacaattta aaacatacat ctggagcagt tgagcatcta
      481 ggagataaca gaactggcga aggtgatgga gatgatgaac aaatagttgt tgatttatct
      541 aagattcctg gagaaataag caagatttct tttactgtta caattcatga tgcgacagaa
      601 agaagacaaa attttggaca agtaagtaat tcttatataa gaatagtaga taaagacaca
      661 aatgaagagt taataaagta tgaattagga gaagatttta gtatagaaac agctatagta
      721 gtagctgaaa tatataagca caatggagaa tggaagttta atgcactagg ttctggcttt
      781 gaaggtggtc ttgctgcatt atgtggaaac tttggaataa acttataatt tcaactataa
      841 caataaacta aagggggata ttttccatgg ctatagtatt aaaaaaagga caaaaaatcg
      901 atttaactaa gggaaatcca ggtcttaaaa atataaaact tggactagga tgggatacaa
      961 attcatttga tagtggctat gattatgatt tagatgtaag tgttttcatg gttggagaat
     1021 ctcaaagagt tgaaaaagat gaagatttta tattctacaa taacttaaaa catccatctg
     1081 gagcagttga acacttaggt gataatagaa ctggtgaagg tgatggagat gatgaggaaa
     1141 tattagtgaa tttagagaca atgccaaagc atatacaaaa gatagctgta acagttacaa
     1201 tatatgaggc tgctgaaaga agacaaaatt ttggacaagt aagtaactca tatataagag
     1261 tattaaattc tgaaaatgat gaagaaatat taagatatga cttaggtgaa gaatttagta
     1321 tagaaactgc aattacagta tgtgagatat ataagtataa tggagaatgg aagtttagtg
     1381 cagttggagc aggatttgaa ggtggcttag aggcactttg tagaaactat ggcttaaatg
     1441 tttaatttaa gcaatataat atattaaata taaataacct atacaaataa tgtataggtt
     1501 atattatata aaattttgtg gaggtttagt atgtcgatta atttatcaaa aggtgataaa
     1561 atagatttaa aaaaatctaa cccaggactg agtaacatac ttgtaggtct aggatgggac
     1621 cctgtgcaac aatctggagg aggttttttt aaatcgttat ttggtggagg acaagctgat
     1681 atagattgtg atgcatctgt atttatgctt aatcaagaag gtaaattaag tggaataaaa
     1741 gatttaattt attttggaaa tctaaaaagt gcctgtaaat cagttttgca tacaggagac
     1801 aatctaactg gtgagggtac aggtgatgat gaacaaatcc tagttaattt ggataaagtt
     1861 ccatcaaata ttcataaatt attgtttgtg gtaaatatat acaattgtgt agatagaaaa
     1921 caacactttg gaatgataga aaatgcctat ataagagttg aagaccaagg taataagaaa
     1981 gagatagcaa aatacaatct atctgataat tattctgaaa aaactactct tatagtagga
     2041 gctatatata gaaaagatgg aagttggcaa tttaaagcaa ttggtgaagg aacaaaagat
     2101 gcgggtctta aagaagtaat gcaaaattta gacaggattg agtgtgcata tggaatttaa
     2161 taatgatatc aaatattata atgtagataa agaagaagtt acaaaagact tatctgttaa
     2221 tcctgaaaaa ggtttatcag aatcagaagt caagactaga agagaaaaat atggtttgaa
     2281 tgaatttact ccaaaagaag agggtagttt ttgggatgat ttaaaggaaa gtttaagtga
     2341 accaatgata gttatcttga ttattgcagc agtagtaagt gcagtaattg gggaaactca
     2401 tgatgcaata gggattgtag gtgctatagc aataggtata gcaataggta tgattacaga
     2461 aggtcgttct aagaaagcag cagaagctct ttctaagttg actgaaaaca tagaagtaaa
     2521 agtattgaga gatggggaag tacatcaaat ttctaagagt gaattggtac caggtgatat
     2581 agtatatatt gaaactggtg acatggtacc tgctgatggt agacttatag aaagtataaa
     2641 ccttaaaatt agagaagata tgttaacagg agaatctgat gacgtatcta aaaagtgtga
     2701 tgtagtagtt tctatggaag agatagaatc taaaggagca gtagtagtac aggaaccaat
     2761 acctgcaaaa cagataaata tggtgtttgg tggtacactt gtagcttatg gtagaggagc
     2821 tctagttgta acttcaattg gagataatag tgaaatgggt aaaattgctc aaaacttatc
     2881 agaaacagat gagcaaactc cacttcagat aaaacttgga aatttaggtg gtttgatagc
     2941 caaagtttct agtgcagtag caggattact atttatattt atggttttcc aaatgataag
     3001 taagaatgta ttaaatgtag atatgtctgg aatattaccg tttttagaat caatagaccc
     3061 agttaaaaca gcatttacag tatgtgttgc gttgatagtt gcagcagtgc cagaaggtct
     3121 tccaactatg ataaatatga cacttgcaat aacaatgcaa aaaatggcta aaataaatgc
     3181 attggttact aaaaaagaag cttgtgaaac gataggttct gtttctgtaa tatgttcaga
     3241 taaaactggt acattaactg aaaatagaat gactgtagaa gttgcatatg ttgatggtag
     3301 atatattgaa agttcagaag aggaaataaa tagttatttt gaagaaaact gtatgataaa
     3361 ctctacagct gatgtagagc acaatgatgg tgatattaag tatttaggaa gtgctactga
     3421 atgtgcattg cttttatatt acaaaaacgt agattataga caagttagaa aaaattctaa
     3481 tatagtagct caaaatcctt ttacttcaga ttcaaagaga atgacttctg ttataggtca
     3541 agataatcat catgtactat tatcaaaagg agcaccagaa gtcttacttg aattatgttc
     3601 acatattcaa agaggaaaag acatagttcc attaactgaa aatataaaac atgagatttt
     3661 agaagaaata aagaaattgc aaataaaatc aatgagaact ttagggtttg catataaaga
     3721 aatctcacaa gctgaagaag aagcagcagt aactgctgaa agtgatgcta tgaatgtaag
     3781 tgcaatggaa aataatttag tatttagtgg ttttgttggt ataagagacc cacttagaaa
     3841 agatgttata gagtctgtaa atacagcaaa taaagctggt gtttctgtaa aaatgcttac
     3901 aggagataat ataaatacgg caagagctat aggtgaagag ttaggcctat taaaaaataa
     3961 tatgagagtg gttgaagctt catatataga tactttagat gatgaagaat taaaacaaga
     4021 gatacaaagt atttcaatcg tggcaagaag taaaccagat agtaaaatga gaatagtttc
     4081 agctctgcaa aagagcggag atgttgttgc agtaacaggt gatggtataa atgatgctcc
     4141 agcactttca aaagcagatg ttggtatagc aatgggtata tctggtacag aggtatctaa
     4201 aaacgcagca gacataattt taactgatga tagttttagt accattgtaa aaggtattaa
     4261 atggggtaga ggaatttatg ataacttcca aagattcgtt caattccaat taactgtaaa
     4321 tgtaattgca tttttagtgg ctataatatc tcaagttatg ggacaagaga tgccatttac
     4381 tacaatacaa ttattatggg taaacatcat tatggatgga ccaccagcac tagcattagg
     4441 tttagagcca gttagggacc atgtgttaaa cagaaaacct gttgatagaa aagccaatat
     4501 aatttctaag tcaatggtgt atacaatagt attaaatgca ttttatataa cagcaatact
     4561 aatgttacaa tctacattta atttcttagg agttgatgaa gcgcataagg gaacagtaat
     4621 gtttgcgtta tttgctttta gtgcactgtt taatgcattt aactgtagag agtttaatgc
     4681 agatagtatc ttccctaatt ttacacataa caaattagca ctacaaataa tagtgttgac
     4741 aggaattgca caagtaatat ttactcagct attccaagat ttctttaact ctgtagcaat
     4801 ggattttatg acatgggtaa aagttttatg tgtagcagca agtgtaattg tggttaatga
     4861 agttgtaaaa tgtataataa gactggtaaa accaaaaaag actgcttaaa acaaattttt
     4921 tagaagaaaa cagactgact caaaatatga attttgggtc agttctgaat aaacatttaa
     4981 attgggagga tagttatggg tgactcaaag gtatttgaaa aaatattctc cagccaatca
     5041 gatagaggaa attacactcc ttcaaaggga tatctaagtt attttatttc gtatattggt
     5101 ttagaagatg aagttttgta caatctcgag atatttaaga caaaacaaaa tattgatagc
     5161 aaaaaagata ttgctttatt tactgatgtt atagcaaatc ctagtgattt tgatattata
     5221 aattatttta aaagtggact tcaaaaatat agaactagca tggaagatgt agatataaat
     5281 attttgggat ttgaagatat tgattataaa ataaagcaag caatggatag agtgttaaaa
     5341 gaagaggaaa aagaatttac taatgataga gttaaacaaa attttatagt gaaaataatg
     5401 gcatggataa aaatttatat cggtgcttta gatataaata aaaatgaagc tccaaaagta
     5461 atattttatg gagatataaa aaaacatgaa gtttatttac tcttaatatt atacttggca
     5521 ggttttgatg ttttatatct taatccaaat tcaaagagta atatagatat tttaaaatca
     5581 gaaagataca atatagagtt tgaagaagcc aacataatag aagaaaaaat ttcttttgaa
     5641 gaaagggtta ttttagggga aaaaatagat aaaagttctg taaaaaaagc ttttactgtt
     5701 ggggcagaag catcaaagcg tataagtgag gaattgttaa atgatgcagg atttataaaa
     5761 ccttggcaac tccaagatag aaaaataaaa aatctacttc taagttcaac tgttgatgaa
     5821 atatcaatat actggaatca accacttaaa ttaagaccag gatttaagtt taatgatgct
     5881 attgtagaag ctccaaattt tctttctaag attaatggaa tatataacga taagaatgaa
     5941 tatattaagt ttttagattt attaagagat tcggaaagtt caacatttat tgaatttaat
     6001 ggagatgtag atagattttc aaaagctttt acaagagaag cttttagttt atccttttta
     6061 ttggattcaa aaggtgcaat agataaaaac tctgttttaa ataataaaga ttattccata
     6121 tcaacactag cattgaatca acaaattatg attttagaga aagtggaaga gttgttagaa
     6181 ggaagtatgt ttttgaatgg tttaagtgga gaagacaaga taaaaggtct atttacagta
     6241 cttcacatgg ataagaaatt tgttcatatg atgaacaatt tcgattactc acttataaac
     6301 ccaaaattga ttatatacat gtacaagagt attgtatttg ataaagaaat agtttttctt
     6361 atgcttttac ttagtaagat tggatttgat attatcatat tatgtccagg aggagaaaac
     6421 aatatagaga atgttataaa taaccaattg attgatgtac acagattgga taaaatggta
     6481 tatgacctta aattaaattc gcttgaaaat gatatacctt tacttaaaaa aatttttgga
     6541 aagaggagac gattttagat ggggataaat ataaatagtt tttcaaaaga taagaactct
     6601 gtagaggtta ctgagaattt gcctacagca gttcaagaag aaaacttcga tataatggaa
     6661 tatacaaata ataaaaaaaa tgaactgaga aagtctaagg aagttgaggc attgacatct
     6721 cttatagaag tagaaaatcc agatacgatt ttacagtttg gtaggaaagc atctgaagga
     6781 gtagcaagag tttctgatag ccttttaaat acaataaaat taaatagaaa tgaagaaaat
     6841 tctaaaatgc tggttcattt aactaaaata atggacaagt ttgatttaga cgattttcaa
     6901 gaaactaagg aaccaaattt cgttcaaaaa cttttcaaaa aagctaataa tgccattgaa
     6961 atgatgtttc aaaagtatga aacattggga ggagaagttg agaaaattca aattgagctt
     7021 gaacaatacg aaagagatat agcactttct aataagcaaa taggtgctat gttaaatgaa
     7081 aactttgagt tttacaatga acttcaaaaa tatattgttg ctggagaaat ggcaatagaa
     7141 gaaatggata atgaaatact acctttcttt aaacaaaaat cagagactag tggagaccaa
     7201 atggatgttg tcaattatca agaactatta aaagtttatg atatgttaaa tcaaagagta
     7261 tatgatttaa ggatagcaga aaatatagct atccaaacaa tacctatgct tagaggtatg
     7321 caacataata attatggttt aattagaaaa ataaatagtg catttgtcgt aactttacca
     7381 gtatttaaac aatgtttatc tcaagctata ttacttaaaa agcaagaatt acaagcaaaa
     7441 tctctaaaag cacttgatga caaaactaat gaattattgc ttagaaatgc acaaaatgta
     7501 agtactcaaa gtgcacaaat agctagaatg gctggaacaa gttctgttca aatagaaaca
     7561 ttggaaaaaa tgtacaatac tatcaaatct ggtatagatg aaactatgag aatagaagaa
     7621 aacaatagag ctttaattaa ggataataca aagagattgg aagaattaaa tacgactata
     7681 atttataata agtagtatta tattaagggc taatttgtat aaagttggcc ctttctatgt
     7741 atatgaggag gctagacatt gaaatatttc gattatatct gcaaagatga tttggagaga
     7801 atttttttga aggagccaga agattttagt gctaagactg aaaaagatgt gttgaaatat
     7861 gcactgggcg cttttttata cgttccagct actcaatata atatgattta taagagtatt
     7921 ataggggatg ttaagggagt tagaccatta gctatttgtt tagaagatgc tgttggagta
     7981 aatggagagt tggaggccat agaaaatctt agattaattt taaaaaatat tagcaatgaa
     8041 agtataacta ataaagatgg aataccacta atttttgtaa gaataaaaga tgtggagcaa
     8101 ctgttgagaa ttaaagagat tataattaaa aatagtcatt ttataacagg aatattaatt
     8161 cctaaagcaa attcggagtt aattgaaaat tgtatagaag ctttagattc tatgaatctt
     8221 caagatatgt atgttattcc gatagtagag acaaaagaat ttatatataa tgaaaaaaaa
     8281 gaacttagtt ttactaattt gtacaatgct attttaagac ataagtcaag aatattaagc
     8341 attagagtag gcataactga tatattaggg atgtatggta taaggagaga taaaaacttt
     8401 tctatatata ataatttgat ttgttcttca tttatattag atataatcac atatcttcaa
     8461 agaccagagt tagatatacc tataagtggt ggagtatcag aattttttga tatgacgaat
     8521 aaagatataa gaaataagta tatagaagaa atattattgg ataaatatca tggattaatt
     8581 ggaaagaccg taattcatcc aatgcaaatt caaattgtac aagcgctatc tacagtatca
     8641 tatgaagatt ttactgatgc tttggatata ctggatagta cagacagtaa gtatggtgtg
     8701 agcaagggtg tattaggaga gagaatgaat gaaacaaatc cacatttttt atgggctaaa
     8761 aaaacattga ttctatctaa aatatatggt gtattaaata aaggagtaga ctatgaagaa
     8821 ttgcttaaat tttaattgtg agattgacat tattgataat ccattgaatt tagaactaaa
     8881 tgatttttta gacgtaggaa taagaaataa tagcaaaaga cgttttttgt ttatctcaaa
     8941 gaaactcggt aaacatttag catgtaagcc aagtgagatg gataatttgg gaaaattaat
     9001 ggctacaatt tataatgaaa aaaataagga agaatcaaat ggtactgtta tagcttttgc
     9061 tgaaactggt acagctgttg ggcatagttt ttttgattac ttatgtggag attatgaatt
     9121 tattcatact actagagagc agtttgacga gttagaaaat ctggaatttt tagaggaaca
     9181 ttcccatgca acagaccaga atttatactt tggtatgctt aacaacttta aatttggaga
     9241 tgagataata ttggttgatg atgagattac cacagccaat acatgcatga atataattag
     9301 gaaaattcaa agcatttatc ctaaaaagaa atacactata tgctcaatac tcaattgggt
     9361 aagtgatgaa aatttaagta aagttactcg tttagaaaga gagctagatt gtaagataga
     9421 ttttgtatac ttatttaaag gaaactttaa attttatata gatgaagata ttatttcaaa
     9481 ttacaacgat aattatagta ttactaaatt agagactatt tcaagtgata aagtaaattt
     9541 agtagtaaat catatttatg ttgatttaga agattatatt ggaaataaga aatatgtaaa
     9601 atatacagga agatttggaa ttaatagaag agaacaattt agattaaaag aaatcgttaa
     9661 gagagaaagt aaaaaaataa ctatggccag taaagaatct ttaaaaaatg aaaaagaaaa
     9721 gattctattt ttaggtactg aagaatttat gtatatacct atgctgtttg caaaagaaaa
     9781 agaaaatcaa tatgatatat actatcattc aacaacaaga agtccaatta ttaatattga
     9841 taagaataat tatcctatta aaagtaaatt tatgctaaag agtttttata atatagatat
     9901 tcaaaattat atttacaata tagataaaca taatttttct aagtgttatt tatttgtaga
     9961 gctgaataaa agtgaaaatt cttatctaga atttattgat ataattaaaa aaacaagtat
    10021 aaaagaatta actatagtat gttgtagcta aagtgtttta acaggataaa atatatcacg
    10081 attttaagat aggagttgag tcatatgaga gattttagca cgtattctaa agatgatgtt
    10141 atttttttat taaaagatat atcaaattta attgaagaag aaggaaataa agaaagagaa
    10201 ttaaaaatac aaaatggtag acattattca gaaatgatac caatagagta tgaagttagt
    10261 gaagattatc tgaatttata tcatgagaaa ctaagagaaa ataaagataa acttgcattt
    10321 tctataggag ttatgtgtga aaaaataatt aaaaaaaatg gaaatgatgt agtactagtt
    10381 tcacttgcta gagcaggaac acctattggt attttggcaa aaagatatat aagaagcaag
    10441 tataatataa gtttacctca ctatacaatc tctataataa gagataaggg aatagatatg
    10501 aatgcaattg aatatatagt aaagaatcat ccaagttcaa aaattcagtt tttagatgga
    10561 tggactggta agggaactat atcaaaagaa cttgaaaaag catgtgatga acttaatgtt
    10621 aaatttaaaa aacattttga ctcaacactt gctgtattag ctgaccctgc tggatattca
    10681 ggcctttatg gaattagaga agatttctta ataccaagtg catgtttaaa ttcaaccgtt
    10741 tctggtttag taagtagaac tgtactaaga gaggatttaa ttggtaaaaa tgactttcat
    10801 ggagctaagt tttatagaca tttaaaagat aaagatgaat ctatgaacta tatcgaaact
    10861 atagaagaat gttttaaaaa tcaatttaag aatatatctg atgaagttga aaactgggaa
    10921 agtgacatta taacaagaga tggatacttt gatgttttaa atataaaaga aaagtataac
    10981 ataacagata ttaactttat caaaccaggt gtaggggaaa cgacaagggt tttacttaga
    11041 agagttccgt ataagattct tgttaaagac ttaaatgata aatctttaga ccatatattc
    11101 atattagcta aggagaaaaa tgttgaagtt gaacaaatgg atttaaaagc ctataaatgt
    11161 tgtggaataa taaaaaatat gaaagatatt taggaaatat attagacagg agaatattga
    11221 atgattgttt tttcagattt agacaggagt atcatttact ccaataaatt tttaaatgct
    11281 gatagtaaat atactaatat agaaatttat agggaaaaag aaatatctta tatatcttta
    11341 gatactataa atttaataaa gcaaatacaa tattatggta tgtttatacc tacaactact
    11401 agaacagtgg agcaatttaa gcgtatagaa tttaataaat atgggattta ttttccttgg
    11461 agtataactt caaatggagg agttatactt aaagataatg aaatattgaa atcatggagt
    11521 gaaaaaatag ataaactaaa atgtgattat gagcccattg aaagtatgat tcataaattt
    11581 aaagactatt tgaatgtaga tggaataact aattttaaag ttgcagaaga tacatttttt
    11641 tatatagttg ttgatttatc aagatttaat ttggattcaa tcaaagaata tacaaatata
    11701 ttggaatcaa aaaattggaa gttttatgtg agtggcagaa aaatatattt tataccaaaa
    11761 gaaatttcaa aagaaaatgc tataaaatac ttgactaaag aattaggaat agaatatttt
    11821 tatgcagttg gagattctat tatggactat ggtatgctta atatctcaaa taaatcctat
    11881 gtattgaagc atggtgatat aaataaaaat gaaatagaaa attcatttat atcaagttct
    11941 ttcaatggaa tgagtggaac tgaagagata ctatctaaca ttttagacga gaactgccta
    12001 cttaacatat aatgactgat atataaataa taaactagaa aaatctgaaa taaattatag
    12061 taaagtttat catactaatt tatttcagat ttttatcaat ttatatttaa gtaagaactc
    12121 aatatgttaa aatgaaggtg gagtcattgc ccagactgag atacatacct catcatcata
    12181 attttcatac acatgtggta tagtactgtt aaaataaaaa ctatctcctt cctccaatag
    12241 atattcctta tttccaagtg ttatcttcat tttacctttt attatataac cacattcttc
    12301 tccttcgtgt gtcacaaatt cactgttttt ttgtgtatga ccatctagcg taacaagtat
    12361 aaattctatt tttttcccag ataaattagg aacaagaagt tcataaaatt ttgtagaatc
    12421 attagttact attttttttc gattattttt ctttactact atatcagagt cttctcttcc
    12481 aatctcttca aaaaaataac ctatatttat atcaagagct tttgatattt tccacattgc
    12541 tacaatagat ggaacaacca tattacgctc aatttgactt attaagcctg tacttaaatc
    12601 tgaatttttt gctaaagtag agatacttat atttttagat tttctaagag atctaatttt
    12661 gcttccaaca tctatattca ttagatacaa accccctcag acatgattat aacatataat
    12721 ttataaagat taataaaata aaaaaaaagt tttacaatac aagtattttt tatgttatat
    12781 tgaattttct gaaaagatgc attataatag tattaatatt caatatattg aatattgata
    12841 acgatatttt aatttaatga aatggaggta gttaagatga agagttttgt aagccctgat
    12901 aacatttggg tattatgggc aattgtaact ggatgggcag catttagtat ttacttggaa
    12961 caaaagtaca attgggcatc aaaagtgtct ggagcaataa tagctcttgt aggagcaatg
    13021 ttattatcaa atctaaatat tataccagta gagtctgttg tatatgacca agtatgggga
    13081 tatgttgttc ctttagccat agctttattg ttatatcaat gtaatattaa aaagatatgg
    13141 aaagaaagtg gaagattgct cattatattt cttgtaggtt ctgtaggaac tgtacttgga
    13201 gctatgattg gatttttagc attaaaaaat gtagtaccag atttaaatat tgttgcagct
    13261 atgatgacag gttcttatat aggtggaaat gtaaactttg ctgctatgtc aggggcgttt
    13321 gatgcgccag gagaattagt atcagcaaca gttgtagcag ataatctttt aatggcatta
    13381 tatttctttg tcttaatagc tataccatca attggatttt ttagaaaaca ttttaaacat
    13441 ccacatgtag atgaaatgga aagtataggt ataactgaag gagaaacagt agcagcaaat
    13501 tattggggaa gaaaagaaat atctttaaaa gatatagcaa tagccattgg tagcgctttt
    13561 ataattgttg cagtatctgt agaactatca acttggttta aagctataat accattatca
    13621 aatccttttt tagcaatgtt aaatacttta tttggaaatc aatatctaat aataactact
    13681 ttgactatgt tgtttgccac atttatgcct aatttctttg gtgatataaa aggagcacag
    13741 gaattgggta catttttaat atacatattc tttgttgtag tgggtgttcc tgcttcaata
    13801 acattaataa ttcaaaaatc accattacta cttttatact gcggaataat ggtactaata
    13861 aacatgctag ttacgtttat agttgcaaaa atatttaagt ttagcttaga agaagctatt
    13921 cttgcatcta atgcaaatat tggtggacct acaacagcag cagctatggc aatatcaaaa
    13981 ggatggtcta agctagtagg accaatactt atagttggta catttggata catagttggt
    14041 aattattttg gtcttttagt aggaaatatt ttaatataat tttacgtgaa aaaaagttgt
    14101 tttttagaag gattttattt aattggaggt tgttatgatt ttcgatggac attcagatac
    14161 ttgggcagat gtaacaataa gaaggcttaa cggggaaaaa gatgtattta gaagaatcca
    14221 tcttgataat tttaaaagag caaatataga aggagctatt tttgtaatat ggatagaccc
    14281 accatatgat aaagagcctg aaaaaagagt taaacaaatt ataagctcta taaaagatga
    14341 aattgaagat atgaaagatg ttgtaaacat agtaaaaaaa tattcagata tagataaagg
    14401 tataagtgaa aacaaaataa atattttaat tggtcttgaa ggattaagcc atattggaag
    14461 aaatatagat ttattagatt atttttataa agaagttggt gcaagacatg catcattaac
    14521 ttggaatgaa cagaatgaac ttgctacagg atgtttaggg gatgaaaatc gtggtttaac
    14581 tgaaataggt aaaaaagcag tcaataaaat ggaaaaattg ggcatgatag tagatgtgtc
    14641 acatttaaat gaaaaatcat tttgggattt aatgtctgtt gcaagtaagc cagttatagc
    14701 ttctcattct aatgctagga gtttatgtga tgctaaaaga aatcttacag ataatcaatt
    14761 aaaagaaatt tcaaatacag gtggattagt tggaataaat agttttagag gatttactaa
    14821 tagtaatcct aagaagcaag atttaataca tttggttgac catatagact atatggtaaa
    14881 tcttataggt atagaacatg tatcttttgg ttttgatttt agtgattact tagggaaaga
    14941 gactataggt tcttttactg gaaatgctag tgtgccaagt gtagatggtc ttgaaagcac
    15001 acatacagca cagaacatag tagtagagct tgaaaataga ggttataaaa agaaagatat
    15061 agagttaatc tctagtggta atttttatag gctctttaaa gatatactaa aataatataa
    15121 taaagaactt aaatttataa aatagtatat ttatacaaat aatatctaca tatttaaatt
    15181 ggcgttcata atttattaaa ttaaatttta atgttactag aagtttcaat tctagtaaca
    15241 ttttttatgt taaaaacaga tatagatatg tattgacaat gataattatt attgataaac
    15301 tattaaagtg aaaatgataa taattttcag tttaattaat aaaagatttt taaggaggga
    15361 gaatgaagaa aaggtattta gtcataggaa taataatttt gtcttatttt tctttattta
    15421 taggagctga agatataaat ataatgcata tatttgccaa aaatcagcat aaacttatga
    15481 tatttatcat gagtagaata ccaagattaa taagtatatt gattgctggt gtaggaatga
    15541 gcatagcagg tcttataatg caacagataa gtaagaataa atttgtatca cctacaacag
    15601 gtgctacaat agatgcagca caatttggga tagtaatatg tatgctttta gtaccaactg
    15661 cctctatttt tacaaagaca ataatagcat ttgtattttc tcttgtaggg acttttacgt
    15721 ttatgaaaat aatagggaag ttacaattta aaaatataat ctttgtacca ttagtaggta
    15781 taatgtttgg aaatataata ggttctatga cggattttat cgcgtataaa tacgatttga
    15841 gtcaaaatgt tagttcttgg atgcaaggtg acttttctat gatacttaaa ggaaattatg
    15901 agatattata tatcacaata ccccttataa tattagcata tatttatgca aataaattta
    15961 ctgttgttgg tatgggtatg gattttgcga caaacttagg tcttagttac aaaagaattg
    16021 tcaatatagg tctgataata gtagcacttg ttacagtatg tgtagtcgtt acagctggta
    16081 atatcccttt tataggtcta atagttccaa atatcgtatc cttgtatatg ggtgataata
    16141 taagagcaag catatggtat actggtttac ttggagcgat atttgtactt atatgtgata
    16201 tttttggaag aattattatt tatccatatg aaatatctat agggcttaca gttggagtta
    16261 taggaagtat actattcctt tatttaatat taaggaggaa tgtgaatgaa gcttagtctc
    16321 aatcaaaaaa tttactttgt aagtatatta ctattcatat caatagcact ttttataatt
    16381 tatggaatag acatgaatca tttagaatat gcattatcac aaagaatacc aaaaatattt
    16441 gctatggtat taggtggagg atgtatagca tttactactg tagtgtttca aacaattaca
    16501 aacaatcaaa tattaacacc aagtgtacta ggtttagatt cattgtatgt aatgattcaa
    16561 actataatag tattcatatt tggttcaagc agtagtctga ttataaatga aaattataat
    16621 tttattatta atgtagctat gatgattgga gcttcattgt tattatataa gacgttattt
    16681 gaaaaaaata aaaataatat tttcttttta atcctagtag gaatgatatt tggaacctta
    16741 tttaaaagtg caacaacatt tattcaggta atgatagacc cgaatgaatt tttagcttta
    16801 caaactagta taatggcaag tttaaacaat ataaacacta atgtattgtt aatagcattt
    16861 ataataatta ttgccataat accttttata tatgatgaaa taaagtattt agatgtattg
    16921 tcgttaggta aagaacaagc aattaattta ggtgtagatt ttgataaagc agttaaaaaa
    16981 atgataatat taatagcaat tttagtctca atatcaacag cacttattgg acctatgaca
    17041 tttttaggac ttttacttgc taatataaca agagaaattt ttaaaacata taagcataca
    17101 tacttgattt cgggttctat gttgataggg atgattacat tgataatagg tcaatttttt
    17161 atacaacatg tatttaagtt tgatacaact ttaagtgtag taataaattt tataggagga
    17221 atttacttta ttcaattact attaaagggg gcaaatagat gatagagata aaaaatatat
    17281 ttaaaagata taagaataaa aatgttgtag atgatgtctc ttttagtata gaaaagggaa
    17341 agataacatc ttttatagga ccaaatggag ctggaaaaag tacagtacta tcaatagtta
    17401 caagacttat aggaggtgat ggtggagaag taataataga aggaaaaagc ttgactaatt
    17461 atagcaataa agaattagca aaaaaaatag ctattcttaa acaatctaac aatataactt
    17521 taaagcttac aataagagaa ttagttggat ttggaagatt tccatatagt gaaggtaatc
    17581 taactaaaga ggatgaaaat tatattgatg aagcaataga atacatgaag ttgactgata
    17641 tacaacataa gtatttagat gaattgagtg gaggtcaaag acaaagagct tatatagcaa
    17701 tggtaatagc tcaagataca gagtacatat tacttgatga accactgaac aatttggata
    17761 tgaaccattc cgtacagatg atgaaggtac ttagaggtct ttgtgatgag ttagataaga
    17821 ctatagtatt agtaatgcat gatataaatt ttgcatcatg ttattcagat aatatagttg
    17881 cattaaaaaa tggaaaagtt gaaaaagtag gtcgtacaga tgagatagta aatgaaaaag
    17941 ttttagaaga tatttatgag atgaatttta acattaagaa tataaatgga aatcgaatct
    18001 gtatttattt ttaaaatatt gtatatgaaa ataaacttgc tatatgattt tttataaagt
    18061 ttttaaatta aaataaagat taataggaga gaggaaaata ttgatatgaa taaaaaagca
    18121 gcaatagtag cagcagtagc tattatagga ttagtgacag tgtttgcatt aggtggaagt
    18181 aaaaagaatg aatcaaaaac atcagaaaac tcaaataata ctatgaagat aactcataac
    18241 ttgggagaaa cagatgtaaa attaaatcct aaaaaagtag tagtatttga ttattcggct
    18301 ttagatacaa tggatgcatt aggtgtagct gagaatttag taggacttcc aaaagcgagt
    18361 ttaccagcat ccttagaaaa atataaggat gacaagtatg cagatttagg aggattaaaa
    18421 gaaccagatt tagagggtat aaagtcggca aatccagatt taattataat aaatggaaga
    18481 caagaagatt tttatgagca attgtcaaaa atagcaccaa caataagtac aagtaaagat
    18541 gataagaagt acttagaatc tgttaaaaat aatatagaca aaatagcaaa aatatttgga
    18601 gtagaagaaa aagcaaatca agaatttagt aaaattgaaa agaaaataga aactttgaat
    18661 aaaaaagtaa cagataaaaa tttaaatgct ttaactataa tggtaaatga aggtaattta
    18721 agtgtatttg gtgaagaatc aagatttagt atattataca atagttttgg atttgaaaat
    18781 aaagataaaa atataaaaga atcaagtcat ggacaaaata ttacttttga atatatagct
    18841 aaacaaaatc cagaagttat gttcgttata gatagaggaa tagctactgg cagtgatgta
    18901 aaagaaagct caactgcaaa atctgttttg aacaatgata taataaaatc tatggatgca
    18961 tataagaatg ataatataat atatttagat tcaccaacat ggtatgtaaa tgatggtggt
    19021 ctaacatctt taaataaaat gatagatgat gcttcaaaag cggtaaatta ataatccgat
    19081 ataattaatc aaaatgtatt ttttataaaa tgattatcaa ataaactagg agtttttaag
    19141 aactcctagt ttattttttg cataaaaaag atgtagttta aacaataata ttatctataa
    19201 taagataaat ctattcaaaa tctaataaaa tgtgaagtta tactagacaa aaagaataac
    19261 tcttaaatat
//
LOCUS       DAEMIK010000075        19247 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEMIK010000075 DAEMIK010000000
VERSION     DAEMIK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.075"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..1074
                     /locus_tag="KPK87_002204"
     CDS             370..1074
                     /locus_tag="KPK87_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2538351.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            1076..2563
                     /locus_tag="KPK87_002205"
     CDS             1076..2563
                     /locus_tag="KPK87_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2538352.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIEFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2768..4381
                     /locus_tag="KPK87_002206"
     CDS             2768..4381
                     /locus_tag="KPK87_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2538353.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4743..5573)
                     /locus_tag="KPK87_002207"
     CDS             complement(4743..5573)
                     /locus_tag="KPK87_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2538354.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5646..7373
                     /locus_tag="KPK87_002208"
     CDS             5646..7373
                     /locus_tag="KPK87_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538355.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7410..9272
                     /locus_tag="KPK87_002209"
     CDS             7410..9272
                     /locus_tag="KPK87_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538356.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9605..10294
                     /locus_tag="KPK87_002210"
     CDS             9605..10294
                     /locus_tag="KPK87_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG2538357.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10399..11085
                     /locus_tag="KPK87_002211"
     CDS             10399..11085
                     /locus_tag="KPK87_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG2538358.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            11102..13009
                     /locus_tag="KPK87_002212"
     CDS             11102..13009
                     /locus_tag="KPK87_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG2538359.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRLLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMSPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13462..14151
                     /locus_tag="KPK87_002213"
     CDS             13462..14151
                     /locus_tag="KPK87_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538360.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14587..15126
                     /locus_tag="KPK87_002214"
     CDS             14587..15126
                     /locus_tag="KPK87_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538361.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            15145..16188
                     /locus_tag="KPK87_002215"
     CDS             15145..16188
                     /locus_tag="KPK87_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538362.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            16190..17026
                     /locus_tag="KPK87_002216"
     CDS             16190..17026
                     /locus_tag="KPK87_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538363.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            17131..17805
                     /locus_tag="KPK87_002217"
     CDS             17131..17805
                     /locus_tag="KPK87_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538364.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIASGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            17805..18857
                     /locus_tag="KPK87_002218"
     CDS             17805..18857
                     /locus_tag="KPK87_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2538365.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
BASE COUNT         7471 a         1959 c         3374 g         6443 t
ORIGIN      
        1 caataaaaag tttgaaaaat atcaattttg atataaactg aataaataca gttgaatatt
       61 aaattattat tttatcaaaa atatgcaaag tactacacct tcaaatatca cctaaagtaa
      121 ttaaaaggca attaaggtga agtagttcaa gtatttctaa aataagttta ataaattcta
      181 taataattta ttttttgcct taaaattgcc ttaaaatcca aacaatttta aaactaatgt
      241 actataaaaa gctggtataa tttaaatata gtaaaaaata agatttatct tatctattgg
      301 gatttaatta tttaatcaat atatttatac catttaattt aaataaaaag tctattaagg
      361 agtgagctta tgagtaagtt aatttatata gcagatgatg aagataatat aagaaatttg
      421 gtaaagacat tcttaaaaaa tgaaggtcat gatgttatgg attttaaaac gggtgatgaa
      481 cttttagaac aatttaatat aaaggagtgt gacttagtaa tactagatat aatgatgcct
      541 ggctcaagtg gttttgaagt atgtacaaaa cttcgtgaga aaagtacagt cccaataatc
      601 atgcttactg caagagatac agatatagat tatattacag ggataacctt aggtagtgat
      661 gattatttta caaaaccatt tagcccaatg tctttggtta tgagagtaaa atctatattt
      721 agaaggatag agtttgaaaa gaaacaaaat tatgataaat atagtaactc gatagatatg
      781 gaattaaaat ttggtgatgt aataataaat aaaaagaata aaatcgttac aagtaaaaat
      841 gtaaatatag accttacacc aaatgaatat aacctattga cttatttatt tgaaaatata
      901 gatagagcag tttccagaga tgaattatta aacaaaatat ggggatacga tatagaagtt
      961 gagacaaggg ctgctgatga tacagtgaag agattacgta aaaaaatttt aaatacaaat
     1021 attttaatag aaactgtatg ggggtttgga tttaggttga aggagaaaag ttaagatgaa
     1081 gaatggaaat gacaagaata gaattggaaa gattaaatcc aaatttaaat taaagttctc
     1141 aaagaaaaat ataagcataa aaactaggat tttgttgagt atactttgtt tgataattat
     1201 agtatttatg ataatattag tatcttttaa tgtatttctt gatacatata taaaaacaac
     1261 tgcgaatgaa gaattgacaa agtctacaga aatagtagaa catatggata gtaattttag
     1321 acctattaag ccaccacaag atgatggaaa gcttcctccc aaagtattat ctaattttat
     1381 gagaaatgta caagataaag ttaggatggc agaaacacaa tcagatgctg atgctatggt
     1441 agttgattct aagtataacc tgatatttcc aacaagagag gatgatttct taaaaaatgt
     1501 tgatgagatg gagctgatta gggagtgtat tcaaaatgaa aaattagatt taaattcaga
     1561 tgaaaataca cggatatcta ccagaaataa ggattattat atttcaactg taaaaattac
     1621 tagtatatat ggtgaacaag acaagtatct tatacttttt atagacatat caaaaactct
     1681 aaatctagca caaaagataa atattgtatt aatatctgta atgtgttttg ctggaatatt
     1741 agcaatattt acagctgtga ttttatctga gaaaattgct aaaccgataa aagagttgtg
     1801 tgagtttgca aaaaaaatgg gtcaaggaga tttcaaaaga acctactttg acttttcaga
     1861 taaagaatta gtagagctct caactgttat gaataagagt gctgagtatc tggataagta
     1921 tgataatgaa caaaaaatat tttttcaaaa tgcatctcat gaattaagga caccacttat
     1981 gtctattaaa ggttatgcag aagctataaa atataatgtt atagatagta aacatgcaag
     2041 tgatataatt cttgaagaaa gtgataggtt aagtgatatg gttgaagatt tattatacat
     2101 ttctaaaata gataatataa ctaaagattt tgaacttgta gaatgtgatt taagagaagt
     2161 actttcaaat tgtggagcta ggcaaaatgt aagagctata aataagggga tagaatttat
     2221 ttatgaattt gatgaagaaa tggttttatt tgaatgtgat gaaaaaaaca tttccaaagc
     2281 atttatgaat cttatagaaa atgctttgag atacgctaag agtgaaataa aaatagtgtg
     2341 taagtataat caaaaaaata tagttgtgat aatagaagat gatggaattg gaatcaagaa
     2401 agaagattta cctcatgttt ttgaaagatt ctataaggga gtaggaggaa atcatggaat
     2461 tggattatca atagttaaat ctattgtaaa taagcatggt gggcgtatat acgtagaaaa
     2521 tggcaaaaaa ggagctaaat ttactatatt atttaaattg taaaaaaatt agagaaaata
     2581 tagagatgtt tgaagttgtt ttttatatat atctgttgta tactgttgaa aaaagaacaa
     2641 tattaagctg acatatatta cgatagttga catttaaagt aaatgcttaa atatagatac
     2701 gaaaggaaaa gtaaaatctg aagtgtattt aggtttaagt aagcttctta ttttataaaa
     2761 aatatgtatg aaagaacagc tgatttttaa tattacaaat ttagtaacgg ttgtatttga
     2821 agcttttgtc atccatatgt ttttatcaga ctttttaggc cagaaagaag ggtatataaa
     2881 agttgtaaga tatgctaaac tgggttttat aatatgttta ggattctgta atttaataac
     2941 tttaaaccct aaaatcacaa tgcctttaat tttcatcctt attttttcaa catcattttt
     3001 atataaagga aatttaaaga cacgattgtt tactactgtt ttattaagta tattctttat
     3061 actgtcagaa attgtagtta caagtatttt tgttttattt gtaaaagaag gctttgaaat
     3121 aatgcttgaa aataatagta taagagtatt ggcgacaatt ttatctaaaa ttgtcttttt
     3181 gttgacttgt aaaattattt gtctatttaa aaaggatgta catttagata tgcccataaa
     3241 gtattggctc cctttatttt taatacctat atttagttta tttctgtctg tttctatctt
     3301 tgatgtaagt aaatttttta gtcttgaaag tttaaaattt ctaagcttaa tatctagtgt
     3361 tggtatttta tatattaatt ttattgtctt ttatttattt aaatttatta tagataaaac
     3421 taaactatct atgaaatatg aactacttga aaaagaaata attcataagg aagaattaag
     3481 acttagtaat gagtgttata aaattattgt tgaacagact gattctgtcg ttttcgaatg
     3541 gaatatcaaa gaaaataaat catttgtatc gcaggcttgg actgaaaaat ttggatataa
     3601 caatgcatgt aagaatattt ttaaagaaat taaagataaa gacttagttc attctgaaga
     3661 taaagctata tttgaaggtt ttcttgaaag tattaaaaaa aagaatatgc acaatcaagc
     3721 agtatatcgt ttaaaaaaat ctaatggtga atatatttgg tgtagaacat caattacaag
     3781 catttataat gatgaaaatg aacttttaag agttgtcgga gtaattgtag atgtagatag
     3841 tgatataaaa aaatatgaag agttaagaac tagagcagaa agtgactcat tgaccaatat
     3901 atacaataaa ggtacatttg aaaaattagt ggaagaaact atagttatga atacaggtga
     3961 taaaaaagat gcattattca ttattgattt ggatgatttt aaagaaataa atgataattt
     4021 tggtcatcca tttggtgatt ttgtactaaa aacatttgct gataagatac aaactagttt
     4081 tggtagtaaa gacttagtcg gaagaattgg tggagatgaa tttgttgtat atatgcaaga
     4141 ttatgttaca gaagtaaatt tgcataaaaa agctaaagaa ttaaatagag tactttcgga
     4201 taattacact gatttgagtt ttagttttga tgcttctgtc agtataggta ttgcaaggta
     4261 tcctcaagat ggaacttcat tttttgaatt gtttaaaaat gcagatagag cactttatag
     4321 cataaaagca tcaggtaaaa attcatattg tttatttgaa gaagaactat atgtacagtg
     4381 aatttgaatt tagattttat gtgagagtaa gatttatata tacttgtggc tgtctgattt
     4441 ataaaatatg ttactttgta tatttttata tgtttcagta aggttaatta acataagcaa
     4501 aggtgtaagt aaatttatat tagtttaagt ttacttacac ctttataaaa atacaaataa
     4561 tagattcatt tacattctta aatgattaaa gctctttacc ttctaaacat gcataacata
     4621 tcgttataat tccagtaaat aagactaaaa tgccattcct aagattacag tttcatcatc
     4681 tattactcca aatgttttta aagtaactta tttcgctttg tatcataata atttatctcc
     4741 ttttacattt taacttctgc tgagatttga gttagatatt cgttagaatc tcgcacactt
     4801 atatcatata gaagatattc ttcataaaca aaatccccaa tacgtaaatt attctgatta
     4861 aaaaaattta ataacttttc ataagtcttg taaatagttt cataactccc tttatgataa
     4921 gcagttacat aaagtccctt tggtttgatt gatacaggat atttcttgta ctctttatta
     4981 acttttgtaa ataaatatga atatgattca aagtctttat taattatatc ttcaccttta
     5041 atgattgagc caatcgtacc ataatcactc aattcatagt tgtccataaa gttaatacat
     5101 tcatataaaa aattttcttc ctcatctcct aaaaattgtt ctttatatgt aacaggtgtt
     5161 ttgaccaagt actcttcttc atgttctttt aaagtaatag tattcgcatc tatattacag
     5221 gcatttttag tgaatgaaat agtagattcc attagagctt gaatattatt tagtttttca
     5281 atttcatttt taatatcaat ggttttcttt tctaaaagct caattagtaa attaggtgtt
     5341 cttttgtcta tataagcttt aatttctttt aaaggcattt ttacttccct aagtgaagaa
     5401 attataccaa aaatgctaag ttgatggtat gagtagtatc tatatccatt ttctttcttt
     5461 aattctggtg agaaaagacc tatatcatca taatggaaaa gtgttttttt attgattcca
     5521 caaatctttg caaattctcc agttgtaaaa taattttttg aaacttgact cataattata
     5581 attcctcctt gactatcctg ttacagtata gtttatcata gtattttgta aaggagtgtg
     5641 attatatgaa aaaattaatt cattttttaa aaccatatag agttttaatt gttgtagttt
     5701 tgatatttac gtttttacaa acacttggaa ctttgtatat accaacactt acagcaaata
     5761 tagtaaataa tggtgtagta aagggagata tagattatat tgtaaaaaca ggtttaatga
     5821 tgatgatagt agctggtata acagctttat cagctgtgtt agtttgtaaa gtatcagcaa
     5881 atctttcatc tggattttgt agagatatta gagaagcagt atttataaaa tcacaagatt
     5941 tatcaataaa tgattttaat aatataggta cagcatcaat gattactagg agtactagtg
     6001 atattacttt aataggtcag tctgtgttta tgtttattca attggtatta ccagcaccaa
     6061 ttataactgt ttctggtcta ttcttagcat attctataga taaagcaatg acaattatta
     6121 ttgttgtagt aatgttttta ttcatgcttt cagcattttt agttggtaaa aagcttataa
     6181 agttgtttaa aatgatgcaa ataaagatgg acaatatgaa tagggtgtta cgtgaggtag
     6241 taactggagt aagagttata agagctttta ataggagtcg ttttgaaaaa aagagatttg
     6301 atagaactgc aatagattat tctgaaacag caatttcaat aaataaaata tttgcagtac
     6361 ttatgcctat tgtaatgtta ataatgaatt taggtatagt aagtattatt tggtttggtg
     6421 ggatgagggt cagtaatgga aatatggaaa taggtcatat aatggcactt gttgaatatt
     6481 gtatcttaat tttattctat ttaatcatgg gagtaatggt atttatgtac atacctagag
     6541 caggtgcatg tgcagataga gtaaatcaga tattagatat agaacctgaa attgtagatg
     6601 gaaatggtca taaagatact gtctcagaga gaggacattt agtatttaaa aatgtaacct
     6661 tttcatatgc acaatcagaa gaacctgtgt taaataatat aacatttgaa gcaaaatctg
     6721 gagaagtaac tgcaattata ggaagcactg gctcaggcaa atctactatt gcaaatataa
     6781 taccacgttt ttttgaaatt caatctggag aaatatctat aaatggtcag gatattaaga
     6841 agattcctca aaaagaactt agagataaga ttggatttgt accacaaaaa gcttttttat
     6901 ttagtggaac aatagaagaa aatataaggt atggaaaaga agatgcaagt attgaagaag
     6961 taaagcatgc tgcaagtata gctcaagctg atgaatttat ttctgatatg gaagacaagt
     7021 atgactcatt tgtagcacaa ggtggaaata atttgtcagg tggtcaaaaa cagagaatat
     7081 ccattgcaag agcgttagtt agaaagcctg aagtatatgt ctttgatgat agtttttctg
     7141 cattagattt taaaacagat aaaagactta gaaaagcgtt gaaaaatgaa atcaaagatt
     7201 catcagctat aattatagca caacgtataa gtacaatcat ggatgctaat cagattatag
     7261 ttttaaatga tggaaaaatt gtaggtattg gaaaacataa agatttactt gaaaattgtg
     7321 aagtttataa gcaaattgcg gattcacaac ttagtaaaga agaacttgca taaaatgaag
     7381 aattaatctg atatttggag gtgtatgata tgagtgaaaa aagaatagat gaacaagata
     7441 tagaagtact tagttttgaa gatggcatgt cagcagaaaa agctaaggat gcaaaaaaaa
     7501 ctactaagag attattaaaa tatatggcaa aacaaaagtt taaaatatta attattttta
     7561 tttcagtatt gataagttca gcgcttacag ttttagcacc tatggttatg ggaaaagcta
     7621 ttgaccaact atttaatgga ataaaaactg cagtccaaac aggtacaaaa ttcagtgtga
     7681 attttagtac tatgggaggg atagtatcaa tacttctagg gttgtactta attagtgcag
     7741 tatttatata tattcaacag tatattatgt caggagttgc acaaaatcta gttcttagca
     7801 tgagaaaaga tttaagtgat aaactcaata aacttcctct taagtactat gattctcata
     7861 aaaaggggga aacacttagt atagtaacaa atgatttaga aaaagtagca gatagtttac
     7921 aagaaggatt aatgcaactt attacagcag tagttacagt agttggttct attgtaatga
     7981 tgataagtat aagtgtacca cttacaatag tatcagctat aacattactt gtttcattgg
     8041 gtattactgt tgtaattgca agaaaatcac aagaaagatt ctctgaaaat caaaaggcac
     8101 taggagagtt aaatagcaat atagaagaga tatttactgg tcaaatagtg gttaaagcat
     8161 ttagtaaaga aaaggatact ataagaaact ttaaaatatt aaatcaaaat ctttataatg
     8221 caagtagaaa agcacagttt tcgtcatatg caatatctcc aattattaga tttataaacc
     8281 taattggcta tgttattata gcagttgtag gtgggatatt tgcatcaact ggagcaatga
     8341 ctcttggtag tatacaagcg tttattcaat atgtaaatca agcatcagag cctactacag
     8401 aaatatctta tattgtaaat atgcttcaag cagcaatagc atctgcagag agagttttta
     8461 cagttatgga cgaggtggaa gagataaagg atgaagaatc ttcaaaggtt attagcatgc
     8521 ctaaaggtaa agtacaattt gagcatgtta aatttggata tagtgatgat tttattctta
     8581 tgaaagatat taatatcaat ctaaatgcag gagataagat tgcaattgtt ggacctacag
     8641 gagctggaaa gacaactctt gtaaatttac ttatgagatt ttatgagata caaggtggaa
     8701 gaattactat tgatggtgtt aatataaaag atttaaagcg tggagaactt agaactatgt
     8761 ttggtatggt tcttcaagat acatggctgt ttaatggaag tataaaagaa aatattgcat
     8821 atagtaagtc agatgccaca atggatgaaa ttgtaagagc agcaaaatca gcaagagtag
     8881 accattttat aaggacttta ccacaagggt atgacactat attgactgag gatgcttcta
     8941 atctatctca aggtcaaaag caacttctaa caattgccag ggcaatactt tcagacccat
     9001 cagtattgat tcttgatgaa gcaacatcta gtgtagatac tagaacagag gtagaaattc
     9061 aaaaggcaat gaataacttg atgaagggaa gaactagttt tgttattgca catagattat
     9121 caactattcg agatgctgat ttaatattag taatgaaaga aggaactatt attgaacaag
     9181 gaagccataa ggaacttatt gaaaagaaag gattctatga agaactttat aacagtcaat
     9241 ttacaagtga atatgatgaa gatgttgtat gattatcaag ttttaggcaa ctaaatgatt
     9301 gataaatatt aagttaaata tagtaacatg taaggttata tggaataaga gtatttctct
     9361 atttcatata acctttttta tttatcaaaa taagataaaa tttcctttct aaattagatt
     9421 gaagaatgta tttatcaata taaaagtaaa ataatcatta ttattagtga agtaaatatt
     9481 gtttgccaca tatatgccat aaagatgcac ttattttacg gagatttaac aaagtagtgt
     9541 aaagtatact ctaaatataa aataaatcaa atagagttaa ataaattgag aggtgatata
     9601 aatagtgaat aaaaaattga actatagatt tgaaatgaag cacaaaataa ctgaagcaga
     9661 tgtgttagct ttaaaatcaa gactctatcc aataatgaag aaagatgaaa atgcatctaa
     9721 agatggaaaa tatcttatta ggagtttata ttttgatact cctgaagata aagcactttt
     9781 ggataaatta aatggcgttg caataagaga aaagtttagg ataagatttt ataataatga
     9841 ctgctcttat ataaggttag agaaaaaaat caaacattac aacatgacat caaagctatc
     9901 aactaattta accaaaaaag aagtaataaa tatactaaat aatgatattg aatttctaaa
     9961 agagtcgaca aattgtttat taagagaatt ttatttgaaa ttaaaatgtg aaagattaga
    10021 ggcaaaaagc atagttgatt acaatagaga agcttttatt tatccagttg gaaatgtaag
    10081 agttacaatc gatagtgata ttaaaacttc tgtaaattcg gtagatttat ttaataaaga
    10141 tttgccaact atttcggtaa ttgatgagaa tatgactgta ttggaggtta aatatgatga
    10201 atttatacca gattttataa aggatttaat tcaaattaat aagactacct ctacagcagt
    10261 ttcaaagtat gcttcaagta gattatatat ttaagaataa aggctatgat ttatataaag
    10321 atatacataa ataaggttaa aaaacaaata agttaaaaat aaataaaata aaaaataaat
    10381 aagttaaaaa taggaaggat gattttaatt atgacattta atgatatttt taaatcaagt
    10441 tttatagaaa acgtatcagg tttttcattt gtggacagtg ctttagcact aggttcagct
    10501 tttttagtag gtttgtttat atatatggtc tataaaaaga cttatatggg aattatgtat
    10561 tctcgaccat tcaatgtgtc gcttgtagca cttacaatgc ttacaacttt tgtaatattg
    10621 gcggttactt caaatgtagt tttatcctta ggtatggttg gtgctttatc tatagtaaga
    10681 tttagaacag caattaaaga cccaatggat ttagtatttc tattttggtc attaggttca
    10741 ggtatagttt taggagcagg tttgattccc ttagctgtca tgggttcaat aattatggga
    10801 ttgattctaa tattcttttc aaataaaaca atatctgaaa ctccatatat attgatggta
    10861 aattgtaata atgaagattc agaagatata gcaactgata aaattaaaaa agtatttact
    10921 aaatatcaaa taaaatcaaa aagtgttaca ccagaaaaag gaatagaatt agtgtttgaa
    10981 gtaagaatga aagatggaga aacaagtctt ataaatgact tgagtcaagt agatggagtt
    11041 actaatgcag ttcttgtaag ttataatggt gactatgtag catagagagg agtaatcttt
    11101 tatgaaagat aaaaaattta cccttcttat ctcgattatg attatatttt tatgtgctgt
    11161 agttggagtt tatagtacat ctagcaacaa aagtgctgat ttatatagtg atgtatatat
    11221 tgaaaaatat tttaacagag acaaggttat ggaagttaat atagagatag atgaaagtga
    11281 cttgaaggat atgaatgaaa atgctataaa agaagaattt aaggttgcaa aagtaactgt
    11341 agatggagat acatatggaa acgtaggtat aagaactaaa ggaaattcaa gtcttatatc
    11401 tgtagcaaat agtgatagtg atagatacag ctataagatt aattttgata agtataatac
    11461 tagtcaaagt atggaagggc ttactcaatt aaatcttaat aactgttact ctgacccatc
    11521 ttatatgaga gagtttttaa catatagtat ttgcgaggaa atgggattag cgactccaga
    11581 atttgcatat gctaaagtct ctataaatgg cgaatatcat ggtttgtatt tggcagtaga
    11641 aggattaaaa gagtcttatc ttgaaaataa ttttggtaat gtaactggag acttatataa
    11701 gtcagatgaa ggaagctcgt tgcaatataa aggagatgac ccagaaagtt actcaaactt
    11761 aatcgttgaa agtgataaaa agacagctga ttggtctaaa atcacaaaac tattaaaatc
    11821 tttggataca ggtgaagata ttgaaaaata tcttgatgta gattctgtcc ttaaaaatat
    11881 agcaataaat acagctttat taaaccttga tagctatcaa gggagttttg cccataacta
    11941 ttatttatat gagcaagatg gagtattttc tatgttacca tgggatttta atatgtcatt
    12001 tggtggattt agtggttttg gtggaggtag tcaatctata gcaattgatg aacctacgac
    12061 aggtaattta gaagacagac ttctcatatc ctcgttatta aaaaatgaga catacaaaac
    12121 aaaataccat aaatatctgg aagagatagt aacaaaatac ctagattcag actatttaga
    12181 gaatatgaca acaaaattgc atgacatgat agcatcatat gtaaaagaag acccaacagc
    12241 attttatact tatgaagaat ttgaaaaaaa tataacatct tcaattgaag attctagtga
    12301 taataaggga tttggtaata aagggtttga caacaataac tctaataaca gtgattctaa
    12361 taataattct aatagtgaaa ataagcgctc tggaaatcaa agtgatgaaa aagaagttaa
    12421 tgctgaatta acatcaagcg tagtcaaagc taatacagat aatgaaacta aaaataaaac
    12481 tacaaatgat agtgaaagta agaataatac agataaagat aaaagtggaa atgataataa
    12541 tcaaaagcta gaaggtccta tgggtaaagg aggtaagtca ataccagggg ttttggaagt
    12601 tgcagaagat atgagtaaaa ctataaaatc tcaattaagt ggagaaactt cttcgacaaa
    12661 gcaaaactct ggtgatgaaa gttcaagtgg aattaaaggt agtgaaaagt ttgatgagga
    12721 tatgagtggt atgccagaac cacctgaggg aatggatggt aaaatgtcac caggaatggg
    12781 taatatggat aagggagata tgaatggtaa aaatggcaat atgaatatgg atagaaatca
    12841 agataatcca agagaagctg gaggttttgg caatagagga ggaggctctg tgagtaaaac
    12901 aacaacatac ttcaaattaa ttttaggtgg agcttcaatg ataataatgt cgattatgtt
    12961 agttggtgta tcaagggtaa agagaagaag atttataaag tcaaaataag attaaaattg
    13021 aatataaata attttaatac tgttaagtct aaatatttac ttagcagtat ttttttagaa
    13081 aataatgtat ttggttaata atataaggta ataaattgat aaaaattaaa tacagcatat
    13141 taataataca taataaagta ataaaaaaac tgtttaagta taataactta tatttagaca
    13201 gtttttttca tttataaata atataaatga aattgaaaac atttgaagga tagaaattgg
    13261 ttggataaat gtataaatta cttcaccaaa aaataatgac aaaaattgta actaagttgt
    13321 aataaaatgg tagttattta gcataaaata atgtatcata gaagtatatt ataaaattaa
    13381 taaaataaga tttttgtaag aaaactgctg gcttttgata tttagagtga gtagttaaat
    13441 tatgtttagg aggggtagaa tttgttgcaa aaagtattat ggaaattttt aaagctagta
    13501 gtcggtttat ttatatgttc agttggtata gtattgacga taaattgtaa ccttggatta
    13561 tctccctggg atgtatttca tcaaggactt tcaaatcata taggaataac tataggaact
    13621 gcttctataa ttgtaggctc tatagtagtt attgctgatg tcgttcttgg agaaaatgta
    13681 ggatggggaa ctgtttttaa catgctacta attggattct ttatggattt acttttatat
    13741 agtaatttaa ttccagaggc tgatagttta tttgtaggaa taattatgtt gatattagga
    13801 ttggttcttt tgtctgttgg aatggtattt tatatgggaa gtggtttagg aagtggacct
    13861 agagatggtt tgatggttgc aattcaaaag aaaacaggaa aatcacttaa attaataaga
    13921 ggtactatag aagttggagc attaatagta ggatttttat taggtggaaa agttggtata
    13981 ggaactataa taagtgcatt tggattgggg tatttcacac aaatggtatt tggcttattt
    14041 aaattagatt gtagtaagat taaacataga tttatagtag atgatataaa atttatcaaa
    14101 gtgtatatga gtggtgagaa aaaagctgct gttcaaaata tagttaagta atttaagaat
    14161 aaattcaacg aaatgttaag tattttaaat taacattttg tttagataga taaattgaat
    14221 attaaaagaa tattaaaata tgaggattgc tagaacacta taaaatatag atttctagca
    14281 attttttatt tgttcaatat ttttttagta ctagaagtct tattctctat ttaatgaatg
    14341 aattaattta taattagaga tactaaactt tatagatatt aatataaaca cctttaaact
    14401 gtgtattttc caaagccttt aaaatgcttg tatttattct aaataaaaaa ataaaaaaat
    14461 gtcaaaaaaa ctcttgtatt atatccaaat tttatatata attacctatg ttgtttagaa
    14521 attttaaact caatgtttag taaacaaaat gtttagtaaa ataaaacaga aactaggagg
    14581 tgcaacatgg atataggtga aaagataaaa agattaagaa cagaaaaaca actgacccag
    14641 gaagagttag ctaatagatg tgaattgtca aagggattta tttctcaatt agaaaataac
    14701 ttaacatcac catccatagc tacccttata gatatacttg aaatactagg tacaaattta
    14761 agggaatttt ttaatgaaat agatgatgaa agaatcagtt ttacaaaaga agatatgttt
    14821 gaaacagaag atgaagattt aaaatataaa ttaaagtggt taattccaaa ttcacaaaaa
    14881 aatgaaatgg agccaataat aataacattg tatccaggag gacagtataa agaagaaaag
    14941 ccacatgaag gtgaagaatt tggatatgta ttagcaggtt caatatatgt ccatataggc
    15001 gaaaagaaaa ataaagtaaa aaaaggagag agtttttact ttagaccgaa agcaaatcac
    15061 tatatatcaa acgagggcaa aactacagca aaagtgattt gggtaagtac gccaccatca
    15121 ttttagaata gaggtgttta ataattggta gaaaatataa tagaattaag ggatatatct
    15181 aaacattatg aagaacttac tgttttagat aatttcaatt tagatataaa gaaaaatgag
    15241 tttctaacat tacttggacc aagtggatgt ggaaaaacta caacattaaa aataatagca
    15301 ggatttgagt atgctgatga tggtaaagtt ttatttgaag gtaaggagat aaataatttg
    15361 ccaccatatg aaagacaagt aaacacagta tttcaaaaat atgctttatt tccacacatg
    15421 gatgtttatg aaaatgtagc ttttggatta aaaattaaaa agactccaaa agatgtaata
    15481 gatgctaaag taaaagaggt tttgaaactt gttgcactag aaggttttga aagacgacaa
    15541 gttgaatcat tgagtggagg acaacaacaa agaattgcaa tagcaagagc tctagttaat
    15601 gaaccaaagg tattattact agatgaacca cttggagcac ttgatttaaa gttaagacaa
    15661 gagatgcaaa tagaattaaa gagaatccaa aaaaaattag gaataacatt tgtttttgta
    15721 actcatgacc aagaagaggc tcttactatg tcagatacta ttgttgtaat gtataagggc
    15781 aaaattcaac aaatgggaac accacaagat atatacaatg aacctaaaaa ttcatttgta
    15841 gctaagttta taggtgaaag taacatattt gatggaatta tgatagaaga ttataaagtt
    15901 aatttctgta atagagattt tgagtgtgtt gataaaggat tcgaaaaaaa cgaaaatata
    15961 gaagtagtaa taagaccaga agatataaaa atggtaaagc cagaagaagg aatgttaaag
    16021 gggaaagtta catctacagt gtttaaagga gtgcattacg agatagaatt gaatgaaaat
    16081 ggaagattgt ggatacttca taatactaaa aatgctgagg ttgggactga actaggcatg
    16141 gatatatatc cagaagatat ccatataatg agaaaagaga ataactaaga tgaaaagaaa
    16201 atctttttta gcatatccat atgtagtttg gagtgcgata tttgtaataa ttcctttaat
    16261 attagtagta ttttttagtt ttacaaaaga atcaggtgga ggttatgcct ttacacttga
    16321 gaattataaa gaagtaattg accctatata tatgaaagtt tttgggcgtt ctattctttt
    16381 agcaggagga gctacattaa tatgccttat agttggttat ccagtggcat atataatttc
    16441 aaaggctcgt gtaagtagaa gaggttcatt aatattattg tttattcttc ctatgtggat
    16501 gaactttctt ttaagaacat atgcatgggt tgctatatta ggaaaaaatg gacttttaaa
    16561 tacattttta ggttggtttg gaatacaacc attagcaatt ttgtatacta attttgccat
    16621 acttttaggt atggtgtata actttttacc atttatggtt cttccaatat atacagcact
    16681 atcaaagatg gataatgatt tgataaacgc tgctcatgac ttgggtgcaa ataatatgac
    16741 agtttttaga aaaattatat ttcctttgag tttgccaggt gtaatgtctg gaataactat
    16801 ggtatttatg ccagcagtca ctacttttgc aatatcaaga ctgttaggtg gaggtaaaat
    16861 aatgctggtt ggagatttga ttgaacaaca gtttacagtt gttggagatt ggaactttgg
    16921 ttcagctata tcgatattta tgatgatagt aatattaata tctatgtcta taatgtcaaa
    16981 atttggagat gaatctgaca aagaaggcgg tgggttacta ttttaaaatt aaagaaaata
    17041 acttctaata tatatttagc attagtattc ttatttttgt atgcacccat atttgctttg
    17101 gtactatttt ctttcaatga ttcaaaatca atggcaagat ggaatggatt tacatggaag
    17161 tggtatggtc aattacttca aaatgagagt ataatgagtg ctttatatta tactattgta
    17221 atagctatat tagcgtctgt aatatctaca atagtaggaa ctataagtgc tataggaata
    17281 cataagatga gaggtaagag taagaaatta atactaaatg taaactatct tcctatttta
    17341 aatacagaga tagttacagc agtagctctt atgagtctgt ttgtatttgt aaaaatggaa
    17401 tttggattta caacaatgct attggcacat ataatgtttt gtttgccata cgttatactt
    17461 tcagttttgc caaaaatgaa gcaattacct gataatatag aagatgcagc tatggacttg
    17521 ggagcaacac ctatatacgc tcttagaaaa gtaattttac cacaaataaa accaggtata
    17581 gcttctggat ttttaattgc atttactatg tcaatagatg actttataat aagtttcttt
    17641 aatgctggaa atggtgtaag caatctttct atagaaatct atggaatggc tagaagaggt
    17701 ataaaacctg aaataaatgc attatcaaca ataatgtttg cagtggttct aggtttactt
    17761 ttactagcta ataaaaaaga atctatagta agggggatta aataatgatt aagtttaaaa
    17821 aagcaatatc ccttatagct gtaggtatga tgacagttac tttatttaca ggatgtaata
    17881 aagcaactga ttctacaaaa gttttaaatg tatataatgt tggagattat atagatgagt
    17941 ctcttataga taagtttgaa aaagaaacag gaatagatgt tcaatattca acttatgata
    18001 ctaatgaaat gatgtatcaa aaagtaaaga gtggtagtac acactatgat ttagtatttc
    18061 cttcagacta tatggtagag aaaatgaaaa atgaaggtct tttggaaaaa ttagatttta
    18121 agaatatacc taatatgaaa tatttagata agagtttttt aaatcctgta tacgacaaaa
    18181 ctaatgaata tagtgttgca tatatgtggg gaactcttgg aatactgtac aataagaaag
    18241 aagttaaaga ccctatggat agctggaata tactttggaa tccaaagtat aagggaaata
    18301 taatgatgtt tgactctgtt agagatacta tagggataac tcttaaaaaa ttaggatata
    18361 gcatgaatag tgtaaatcca aaagaaataa atgaagctaa aaatctttta atgaaacaaa
    18421 aggatttagt attggcttat gtaaatgatg aaggtaagga tagattgctt ggtggagaag
    18481 tggcaatggg tatgctgtat tctggagatg cagttacact aatggagcaa aatcctgacc
    18541 ttgattatgc tatacctaaa gagggaacta ataaatgggt agatgctatg tgtgtaccta
    18601 caacagcaca aaataagaaa gaagctgagc tgtttataaa ctttttatta gacccagaga
    18661 atgcaaaagt aaatgctgag tacattgggt attcaacacc taatacagga gcactaaaat
    18721 tattagaccc agaaataact cagaatcctg ttgcatatcc atctaaagaa gtacttgata
    18781 aatgtgaaac ttttgaagat attggtgaaa atataaaatt atatgacaag gcttggatag
    18841 agttaaaatc aaaatagggt cttgttgttt taagtaaatt tacttaataa atacttaata
    18901 aaactgttct tttttagaaa atttattttc taattattga acagttttat tttttgttta
    18961 agttatattc atatgcaatt taaatagttt attaataaat gaaaacttat ttgtataatg
    19021 actttaataa taattagaaa aaatgggcaa cttaatagaa taattggagg tgtaaattta
    19081 taaaaaatga ataggataaa taaaaaaata gatttattaa tattggcttt gctcgttttt
    19141 atatttttaa ttgttggaat agtaatacta tcgattagga cagaaaataa attgaatgtg
    19201 ttcataatgt tagcaataat tttttttata ataatgttaa cttattt
//
LOCUS       DAEMIK010000076        19163 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEMIK010000076 DAEMIK010000000
VERSION     DAEMIK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.076"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            55..393
                     /locus_tag="KPK87_002219"
     CDS             55..393
                     /locus_tag="KPK87_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG2538366.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(520..1809)
                     /locus_tag="KPK87_002220"
     CDS             complement(520..1809)
                     /locus_tag="KPK87_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2538367.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1889..2593
                     /locus_tag="KPK87_002221"
     CDS             1889..2593
                     /locus_tag="KPK87_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBG2538368.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2685..3731
                     /gene="selD"
                     /locus_tag="KPK87_002222"
     CDS             2685..3731
                     /gene="selD"
                     /locus_tag="KPK87_002222"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2733..2735,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG2538369.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3749..5161
                     /locus_tag="KPK87_002223"
     CDS             3749..5161
                     /locus_tag="KPK87_002223"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG2538370.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5275..7158
                     /gene="selB"
                     /locus_tag="KPK87_002224"
     CDS             5275..7158
                     /gene="selB"
                     /locus_tag="KPK87_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG2538371.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEENIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7263..10001
                     /locus_tag="KPK87_002225"
     CDS             7263..10001
                     /locus_tag="KPK87_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG2538372.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIVSGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10120..11064)
                     /gene="manA"
                     /locus_tag="KPK87_002226"
     CDS             complement(10120..11064)
                     /gene="manA"
                     /locus_tag="KPK87_002226"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG2538373.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11237..12598
                     /locus_tag="KPK87_002227"
     CDS             11237..12598
                     /locus_tag="KPK87_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2538374.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12686..14575
                     /locus_tag="KPK87_002228"
     CDS             12686..14575
                     /locus_tag="KPK87_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2538375.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14590..15045
                     /locus_tag="KPK87_002229"
     CDS             14590..15045
                     /locus_tag="KPK87_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2538376.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDERLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15104..15421
                     /locus_tag="KPK87_002230"
     CDS             15104..15421
                     /locus_tag="KPK87_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2538377.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15446..16708
                     /locus_tag="KPK87_002231"
     CDS             15446..16708
                     /locus_tag="KPK87_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2538378.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16813..17877
                     /locus_tag="KPK87_002232"
     CDS             16813..17877
                     /locus_tag="KPK87_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2538379.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17931..19109
                     /locus_tag="KPK87_002233"
     CDS             17931..19109
                     /locus_tag="KPK87_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG2538380.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7714 a         1986 c         3349 g         6114 t
ORIGIN      
        1 tatataatat aaataataat gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac
       61 ttattagtga ttggtggtca tgaaagaatg gaaaaagatt atatggttat ggccaagaaa
      121 aaaggttata agacaaaggt atatactacc atgtcatcta aattaaataa ttctatagga
      181 agaccagatg cagttgtcat acttacatca actgtttcac ataagatgtc tagaacggtt
      241 gagtctcaag ctaaaaaaca agatatatta atcgtaaggc ataaaaatag tagtaaggta
      301 gcatttaatg aatgtctaga tatggttgat gaatgtctgg gaaattgttc aaaatgtaag
      361 cttaaagaca aacaaaaaca aaaaaatata taggtataaa aaatacataa acataaaatt
      421 aaaagtactg agataatagt tataattatt atctcagtac ttttagattt ataaggataa
      481 gtatattata aattttttat tataatttct aactacttgt tactttttca tatttttttt
      541 acttctagct ttttttcttt ttttcttctt tatatttgaa gctattataa ataaaactaa
      601 aagcactaga atacctgcaa taatcttaac aaaagtcatt aaaacattat tttcagtcaa
      661 aaatggaaga ctgctatcaa gattattttt tgcaattaag tctatactgt tttctagttt
      721 accattatta tatacattta aagtaccaac cttagctcct ttttttatag gcaaatcaat
      781 tttatttaac tctgttttgg tatcataatt gccttgagat tcatcttttt ctaagaatat
      841 cctataacta ttttttggtt cataaatcaa ttcatgttgt tttgtaaaca atactttttt
      901 agtatctgtg tatttttctt tatcaacaat agtagcacac ttaaagttat caaatccata
      961 atcaatcaga atacgtgagt ctaaatataa atcagcatta gttgaattaa atacagcaac
     1021 gataactctt ctgccatctt ttacagcact cgataaaaga catttaccag cagcatcagt
     1081 atatccagtt tttataccat caactatatc atattttata tttataggtt gacctttgta
     1141 tgtaatctta tcatgtgatg tcaaaaactt attagtgttt acaaagtatc tctcataagg
     1201 atatgctttt gtagcttcaa atttaagtga cttagttttt acaatttgtc taaaagtatc
     1261 attattcata gcttctctag ctatgagagc catatcatga gctgttgtgt agtgatttgg
     1321 gtctggtaat ccatgaggat tattaaaatg agtattagta gctccaattt tttttgcctc
     1381 agagttcatg aggtcaataa atttttgtac attgccacca ccaacatatc tagctaatac
     1441 aaaagcagca tcattagcag aatgaaccaa aagagcatct agtaactgct ttacagtaaa
     1501 agattcccca ttttttagat acatactaga gccatctatt tgaggtaaat ctttaatttc
     1561 tattacttga tttaaatctt taacctcctt tagaactaaa caagctgtcc atactttagt
     1621 tgttgaagct ggataaagct tagaatttcc atccttatta tatagtattt tgccagactc
     1681 atagtccatt aaaactgcat gtttagcaac tatagctggt tcattatctg caaaagagat
     1741 attagagctt cctaaaaatg aagtaaatat caataaacag attaataaaa gtgataaatt
     1801 tcttttcatt gtgagcctcc aaattttaaa ttcatctttt agtatagcat atatttgtca
     1861 ataattaaaa gaaaggagtg attaatatat gaaaaaaaag gtaaaattta tttcgttttt
     1921 tgttttattt attttaataa tatcatcaat aatcgtttat aaatcgctta ttttcaaaga
     1981 tgatatttat gttgtaagta aaaaaaattt acctaatgaa aaaacacatg ttgtagagaa
     2041 tgaaattaaa ggtgaagata ataatcacaa taaagtaact aaagacatag ataaaaattt
     2101 tcaaaataat aaaataacta tatacataag tggagcagta aatagacctg gaattgtaac
     2161 tatagagtca gacaagagac tttatgatgc tgtagaatta ctaggaggaa cgacaaaaga
     2221 agcagattta aatggagtca atctatcagt aagattagaa gatgaacaac attacataat
     2281 acctaaaata ggtgaagcaa catctgttac aagtaatgat gattccaata aaccaaatca
     2341 gaagaatgag tcaagaggtg agcctaaaaa taaatccaat aaaaatatat ctaacgaatc
     2401 aaaagtaaat ataaatgtag cgactataga agagttggat tctctacctg gggttggaga
     2461 agctacagcc aataaaattt tacaacacag agaagaaaat ggacagttta gttcaattga
     2521 ggaaataaaa aatgtaaatg gtattggaga taaaaagtat gaaaatataa aagatttaat
     2581 ttgtgtagac taaaatataa tacaaaactt acatatagat atgttattat aaatatgtat
     2641 gtatatgtgt gtacatgtgg aaatttagta aaggaggcac ttttatgaaa aaagaacaaa
     2701 aaaaacttac agagatgaca acagcaggtg gttgagccgc taaaataggg ccagaggttc
     2761 tggcatcagt tttatctcaa ttgcctaaaa atgataatat agaaaattta ttagtaggac
     2821 tagatacagc agatgatgct gcagtttata aattaaatga tgatatggca cttatccaaa
     2881 ctttagactt ctttacacca atggtagatg acccatacgt atttggtcaa attgctgcat
     2941 caaattcact atcagatgta tatgctatgg gaggcaaacc tttggttgct atgaatattg
     3001 tttgttttcc atcatgccat gatatggatg tacttgcaga aatattaaaa ggtgggtttg
     3061 ataaagtgaa agaaagtgga gcacttttgg ttggagggca tactgtagat gacaaagagc
     3121 ctaaatatgg tctttcagtt tctgggattg taagtccaaa taaagtttta tcaaatgcta
     3181 ctgcaaaacc tggtgataag ttaattataa caaaaccaat tggagtgggt gtattaaata
     3241 cagccatgaa agaaggtatg gtagaacaac atatagcaga taaagtaata gaaataatga
     3301 tacatttaaa taaatatgca gcaatgagtt ttgagaaatt tgatgtaaat tcggttacag
     3361 atataactgg ttttggtcta cttggtcata ctttagaaat ggccaaagct tctgaagtaa
     3421 gtattgaaat tgaatcaaaa catgtaccaa ttattgaagg tgcaatagaa atggcacaaa
     3481 tgggaattat acctgctggt atgtataaaa atatgcatta tgtatctaaa gatgtagaag
     3541 ttgtaggaaa tatagaagtt gcagttcaag atattttata cgacccacaa acatcaggag
     3601 ggttgttaat ttcagtaaaa gaagaacttg cagaggaact tgtaaaagat atgaaattaa
     3661 atggagcaat agaagctaaa attataggtt ctgtagttcc aaaaggggaa aaatatatta
     3721 cagttttata aaatattata gagaaagagt gattgttttg agcaaaagag aattgtttgc
     3781 aatgttgcct tcagttgatg aagtattaag tgataataga attgtagaaa taataaatga
     3841 atatcctaga agtttagttt tagaatctgt gagagaagta atagatttaa aaagacaatt
     3901 tatattaaga ttaaaagaag atgcatcaaa ctctgtaaca atagagtttg gtgaaataat
     3961 agaatctgca atagagagag ttaagttaaa ttactcatta tctttaaaaa aagttataaa
     4021 tgcaactggt acagttattc atacaaattt aggaaggtct ttacttagtg aagatataaa
     4081 agatgaatta tggtgtgctg cttcaaggta ttcaaactta gaatacgatt tggataatgg
     4141 agaaagaggt tcaagatact ctcatttaac tagtacaata aaaagactta ctggagcaga
     4201 agatgtacta gttgtaaata ataatgcagc ggcagtactt cttgtactta gcacaatggc
     4261 taaaggagga gaagcaattg tatctcgtgg agaattagtt gaagtaggtg gttcatttag
     4321 gattccaagt ataatggctc ttagtggtgc agaattagtt gaagttggtt caactaataa
     4381 aactcatttg aaagattaca aggaggccat tacagaagat acaaatgtat tgatgaaggt
     4441 tcatacaagt aattatagaa taatgggatt cacagagagt gtttctatag aagaattggt
     4501 taatttaggt aaaaaatata aactacctgt aatagaagac ttaggaagtg gagtttttat
     4561 agatttatca aaatatggtt tatcttatga accaactgta ttggattcta ttcgacaagg
     4621 tgcagatgta gtaactttta gtggagataa gatgcttgga ggtccacaag caggtattat
     4681 agttgggaaa aaagagtata tagaaaaaat gaagaaaaat caacttacta gagcacttag
     4741 agttgataag cttacaatat gtgctcttga agcaacattg agaatgtatt tagatgagac
     4801 aaaagctata gaaaatatac ctactttaaa aatgttgaca tataaaattg aagaattaga
     4861 agtaaaagca aataaactat ttgaaaaaat aactgcttta aacttaaatg caaacataaa
     4921 tatagaagat ggattttcac aggtgggagg agggtctatg cctcttgaaa ctataagtac
     4981 aaaggtaata tcaataactc ctgaacatat gaatgtttca tctttagaaa aaaaactaag
     5041 acttagtgaa gcacatataa tagccagagt gtatgataat aaatatgtac tagatgtaag
     5101 aactatattt gatgatgaat ttgatgtaat agttgaggaa ttgagaaaag catttaatta
     5161 aataaatttg agttaataaa tattaaattt agaagtataa gtatgaagtt ttaattaaaa
     5221 aatagctaca cacacttgtg actttagtca tagcaggttt agagggggca aaaaatgaaa
     5281 aatgtagtaa ttggaacagc aggtcatatt gaccacggaa aaactactct tataaaagca
     5341 ttgactggaa gagagactga tacattagat gaagaaaaaa agagaggtat ttcaataaac
     5401 ttaggattta cttattttga tttaccaaac aatagaagag ctggaattgt tgatgtccca
     5461 ggacacgaaa aatttataaa aaacatgtta gctggagcat caggaattga tatagtttta
     5521 cttgttgtag cagcagatga aggagttatg ccacagacag ttgagcattt agatattcta
     5581 agctttttaa atatcaaaaa tggaatagtg gttctaacta agtctgatac tgtagatgat
     5641 gaatttagag agctagtaaa agaagatatt tgtgaaaaaa taaaaggaac ttttcttgaa
     5701 gatgctgaaa taattgaagt tgattctata tctaaaagag gtatagacac tcttattgaa
     5761 aaaatagata aaatgagtga tgatatagaa gataaaaatg aaaattctcc agcaagactt
     5821 aatatagaca gagtattttc aataaaaggg tttggtactg taataacagg cactttaata
     5881 gaaggaaaga taagtataga agatgatttg gttatatatc cacaggtatt aaaaacaaaa
     5941 ataagaagta tacaagttca tggagaaaat aaagatacag cttatgcagg tcaaagaaca
     6001 gcaatcaata tatcaaatgt aaaagtggat gatataaaaa gaggagatgt tttagctgca
     6061 ccaaactcat tggaggaatc aatgatgtta gatgtaaaat tatcattggt aaatcattta
     6121 gataaaggat taaagcactg ggatagatta agattgtatc atggaactag agagatactt
     6181 tgtagagcag ttccactaga taaggaatta attgaaagtg gagaaagtgg ctatgttcag
     6241 ttgagacttg aagaaaacat agtatctaag aaaggtgata cgtttgtagt gagacgttat
     6301 tctccaatgg aaactatagg tggaggtgta ataattgacc catctcctaa aaaacataag
     6361 aaatttgatg aaaaagttat agaagcatta aaaataaaag aaaaaggcga actaaaagat
     6421 ataatagaag agtacttaaa aaggaattta aaaaattatc caaatataaa agaaataatg
     6481 agctatagtg gtgctcatga agaagatgta aaaagagcgc ttgaaacact tatatcagaa
     6541 gataaagtat ttattatagg aaatatgtat atgcatataa atcaatacaa taaattaaaa
     6601 gaaaatacaa taaagctttt aagtgagtac cataagaagt ataggcttag aaaagggata
     6661 ctaaaagaag aagtaaggtc taagatagag agtaatttta agactaggga gatggatatt
     6721 ttattagaaa aattgtctac agaaaatgct ataaaaatag aaaataatat agtatctcta
     6781 ttagattttg aggttatttt aaatgataag caaaaagaaa tagcaaaaaa aatagaaaaa
     6841 agattaaaat catgtggagt ttcttctata ctgacaatag atgaagtttc tgaaggaaat
     6901 cataactatg ctgaagtatt ggagtcaatg ataggtaata aagtagagaa attagatgat
     6961 ttgtatataa tggacaaaga tatttatgaa aatgcaaaga acattttgat aaattatata
     7021 aaagaaaata aagaaataac attaggagaa tacagagatt tgattgattc tagcagaaag
     7081 aattgcatga ttatattgga aaacttcgat agaaacaaaa taactaaaag agtagaaaac
     7141 aaaagaattt tgttttagca gaaaatgtct gtttttgctt taaggtataa agaaggaatt
     7201 ataatgtcga gtatcgtata attaattagt ataaaatatt aattaagagg aagtgtaaag
     7261 tattgaagaa tataatacct gatgtaaaac cagatataaa agaaagagaa gacatgagcc
     7321 ttttatttat aataatgggc tcagtacttt tattatttat tataaaagat atagagaaat
     7381 atgaaacttt attacatgcc atatatacga tatttttagt agctacagga gtaattatat
     7441 ttaatactac aaaatttaaa gtagattcat tttctacatt tttaggatta atttttatag
     7501 ctactggagt attggagtgt atatatttat ttaattcctt ggggattaaa actaagtcta
     7561 taatggagat taacatcaca atatcagcaa taacagattt atttcctata ctgggtgtat
     7621 acttaagttt taaatttgtt aaggataaca aacaaattta ttcaagtgta attttattta
     7681 tagcaactgc tacattaaca ataacatctt tattcataat atcaggaatt tgtgactatc
     7741 tgggaaatgg taaatcacaa tatgctttag gagttataat atctatattc ataataatta
     7801 tttctattgt tagtggaatt gaattaaatt caagttcaaa taaaggcaaa tgggaatatg
     7861 atgaaaaaaa gatttttaac agaataatag taataataat cttatcaaga gttccaaatc
     7921 tattgcatat agttattgat aatagaaatg tagagaggat attatcccag ataataataa
     7981 atgtagcctt atattattta tataattaca tagtatctaa gaatattaag aaaacagcac
     8041 tggaattgca tgatacgaat gaagaattaa caaaaaaaac ggaaagttta aaagaaaaga
     8101 ataaaaaatt aatctatgag acagaaaaga tagaggaact taagggaata cttatgaaaa
     8161 gagaatctag gctacaatct acattagatg tagcagtaaa tagcatagta gtatttagca
     8221 aagatggaga aattacatat gcaaataaga gctttagaaa tacttttgga gactataaag
     8281 aacaagataa attgactgat aaaataaaaa acttcaatga tttgatagaa agcattcatt
     8341 atgtgtttgt tcatgagaag aatgcagaga aacttattag tacaagcgat aataaagttt
     8401 atcaagctat atttgcacct ttaattattg cttcacaaac agaaggagta ttgtgtgtac
     8461 tagaagataa gactaagaaa aaggagtacg aacgcaaact tattgaagca aataagagaa
     8521 gtaaagattt tttagaaagt attggagatg gcatagtagt gcttgaaggt aataacaaaa
     8581 tatatgttaa taatgcatgt agggaaattt ttaaaaataa tttagagagt attgattttt
     8641 cattagtatg taaaagtgaa gaaagtatag aaaaaagata tgtaatagat ggtgaagtaa
     8701 agtatgtaga gatgagtttt tctcagtata ctaatgctgg tactaataaa actattattg
     8761 taatacgtga tacaacctct cgaaaaatat ctcaaataaa acttgaggaa agtcaatcat
     8821 catattcaag atttatagat atattacctg atggcatatg cttattaaaa gaagatttaa
     8881 gtataaacta tgcaaataag tcgtttttag atatgctagc ttttacaaac atagatgata
     8941 taaaagattc taatattaaa ctattaatga atgcgaatac tgaagaaaaa atgaaattta
     9001 cggataatat ggctaaagtt ttaaatgaaa atagaagtat acttttactg gaatatgaat
     9061 tgataaatag ttatgatgat atagtagaag tagaattaag tgcattacct tttgctatat
     9121 acaatactag atacataatg cttattataa aagatttagt acataagaaa tattccgagc
     9181 aagcagaaaa agaactgctt gaaagattta aaacagataa aatcaaaacg gagttttttg
     9241 caaatatgtc tcatgaatta aaaactccac taaatgttat atctagttca aatcaactgg
     9301 tagattcttt ctatagaaat gaaaagataa aagattacaa taacaatatt aaatctcatg
     9361 tggatttagt tagacaaagt tcttataggc ttcaaagatt aataaataac attatagatt
     9421 taacaaagat ggagtctggg ttctatacac taaaacttgc gaaatacaat attgtatctg
     9481 taatagaaga cttatttatg aatatagaag agtatgcact tagaaaagac ataaaaattc
     9541 tttttgatac agaccttgaa gaaattaatg tttatataga caaggttgaa attgagagaa
     9601 ttatgttgaa tctattgtca aactgtatta agtttactga taatggaggc tggatatatg
     9661 taagtattca ttataaaata gataaagtaa tcataagtgt aaaagacact ggagttggaa
     9721 taccacaaga taaattagaa ttgatttttg aagaattttc acaagttgat aagactttat
     9781 ccagaaatac tgaaggaagt ggaatagggc ttgctatagt taagaatcta gtaagtcttc
     9841 atggtggaga tatagaagta gtaagtgaag tgaataaagg cacagagttc ttaatttcac
     9901 ttccaatgaa gggatttagt agtgaacatt acacagaaga taagagaata tataatattc
     9961 aagaaaaaat aaaaatagaa ttttctgata tatactatta aaaatgatta tgagcaaaaa
    10021 taaaataaaa atactatttg tttaaatatt taagttgtaa gaaataggct atcataaggt
    10081 aaattattat gatagcctat ttttagctag gttttatatc tatatgtgag aacatataat
    10141 ctctaaatta ccttcaaaac taaaatctcc aaagtcgttt ggtagtataa aatgcatacc
    10201 cttttttaaa ttatattcta cagaattgtg tataagtttt ccatttccat ccaacacact
    10261 gtataaagaa aaaggtgtat tgtgtgtaaa attacatttt ccaaaaacat ctaatttata
    10321 aacactaaaa aattcgtttg acacaaatgt tgtgcattta aagtctgatg ttgatacgat
    10381 tttatagtca gtatcgaaat ttatatgagg aacattcgta acatctattg atttttgaac
    10441 atgaagttca cgcttattac ctgaattgtc agttctatca taatcataga ctctataagt
    10501 agtatctgag ttttgttgag tttctaaaat caatgttcct ttgcatatag catgtatagt
    10561 accacttgga acatagaaaa agtctccctt ttttatgtta acctttctaa gtagtttgtc
    10621 ccattcatta ttatttacca tatttacaaa ttctttatga gattttgcat tatgacctat
    10681 tataatttct gcatcatcag aacaatctat tacataccaa cactctgtct tacctagctc
    10741 tccattttca ttttttttag catattcatc atttggatga acttgaactg ataaattatc
    10801 atttgcatcc aatatttttg taagtaaagg aaacttatca ccaggtgtgt ttccaaataa
    10861 ttctctattt ttattccaca attcagatag ttttttacct ttatattttc cattttctat
    10921 taagcaatct ccatttttat gagagcttat agcccaacat tcaccagttg ttggcgaatc
    10981 tatttcataa ttaaatttat ctttaagtgc agtaccaccc catattctat ccataaaaat
    11041 aggttttaaa aataatggtt ccataatcct gttcctccta tgatattaaa tttatcctac
    11101 aaataattat atataaatag attataaaat caatagtaaa aaagttttgt cccttgaaat
    11161 gacataaagt ataaggagtt attaaataag ataaactatg atatataata taaagttcta
    11221 aattggagtg ataattatgg agttgtttaa ggcagaaagc aaaaaatata aaaaagaatt
    11281 tttaaaatta ttggagcaat gggttagtat accttcattt tatgatagaa aaactgtatc
    11341 taaagacatg ccttttggaa aaggagtata tgatgcatta aactggtttg aaaatctagg
    11401 tagagaaaac aatttcaaag taaaaaatat agataatcat gctgttcaaa ttgaatatgg
    11461 aaatggtaaa gagtatgtag atatatttgg acattgtgat gttgtaaatc caggtgaagg
    11521 atgggatagt gaaccattta aactaaatat tataggtgat aaattagttg ctagaggtgt
    11581 atctgataat aaagggccta tgatagtgaa ttttttagca ctaaaaatga taaaggattt
    11641 agatattaat ttaaaaagaa aggttagatt aatagctggg ggaaatgaag agagtggatt
    11701 taaatgtata aagcattatt atagcaaaga gccatatggt gtatgtggat ttactccaga
    11761 tgctaaattt cctgtattaa atggagaaaa aggtggagca ataataaagt taatttcaaa
    11821 tatagacgat aaatctttat atataagtgg tggaatagag tttaatacaa taccagataa
    11881 agtttatata aagaatgtag aaaaattggg taaagataat atatgttttg atattaacaa
    11941 tatatcgata aattatgata atggcaatta tattgtgtat ggaaaaggtg gtcattcatc
    12001 taaacccgaa aaatcaataa atcctatatt agctacaatt aaacttttgt cagaaaatat
    12061 tgatgaaaaa tggacaaaag atttatataa gttgataaat caagataata taaatggaaa
    12121 cttatttggt ttaaatattg aggggaaatg tgggatttta tcaatggtgc caacaataat
    12181 aaatatagta gatggaaaat tagaagtagt tttaagcgtt agatatccag aaatattaac
    12241 cattgaagat ataataaaaa aatttaattt gtatatggag caaaataata ttaataaatt
    12301 tgagcttatt ggggaaaatt taaagcaagc aaattatata gatagaaatt caaaacttgt
    12361 aagaagtttg catgatatat atataaagta ttctggagat ttaaaaaatg atgttagagt
    12421 aactagtgca ggaagttatg cttctgaaat gaataattca gtaatatttg gttgtgaatt
    12481 tcctgatggt agctttggca atgtccatag tgcgaatgaa tttgctagct tagatagatt
    12541 tataactgca ataggtatat atgcagaggc aataattaca ttatgtaata aaatataaat
    12601 acaaaatgtc ccttatatag acatattata tatacaatta aaattttact tgatatataa
    12661 ttgaaatcaa agagaaggtg atacaatgat aactaagaaa catgcaataa tagtaaagag
    12721 tttaagcaat aaagatggat atatgacatc aaacgaatta gctatcaaat tggatgtgtc
    12781 tactaaaact attaaaagat atatagcaga tttaaatagt gttttaagca agtatgattt
    12841 ggtaatagct tcttctagag gtataggata caagttaagc ggttctaaaa acaatatagc
    12901 cagagctatt aaagaagcaa ataaatatat tgatggtttt ttagatgact ctgaagagtc
    12961 aagaatgtca aatataatat gtatgcttat taatagaaat tatatgagta tagaagcaat
    13021 ggcagaagaa ttaaatctaa gtatagccgc tataaataaa ttgtctagta agcttaaaag
    13081 aaaacttgaa aagtatgact tagttataaa atctaaacct tactatggaa gctatataca
    13141 tggtgaagaa attaatataa ggcaattaat tactgactat gcaataaaat tagatgaaaa
    13201 aagtaaagtg aaggtgtttt tagatgacat tagtgaaaac gatatacact gtatagaaag
    13261 tattttagaa aagcacttaa gagaaaaaga tacaataatt tcagataaag attttaatct
    13321 tttgctttca aaaataatcg tttctgtatt taggagtaag agaggtcata gtaacaatat
    13381 aaacttaatg gatacaagtt atagatttca taactactac tttatagaaa atctaatgaa
    13441 agaaatatct gataaaattg gatttaaact tattgaagat gaagtaattt atatttcaaa
    13501 ttattctggt gtaatagcct ataaaggtac acaaggaaga aaaaacacaa gtgaaattga
    13561 ggaacgaata agtagtgtta taagtagtgc acttcaagat atattcctta tttcaggtag
    13621 tgattataca aaagatgatg agtttatggt tgcaatctct gaccatataa agagattttt
    13681 aaatagagct agagctaatg taaaatcaaa taatccttta cttcatcaaa taaaagagaa
    13741 gtttcctata gcttttaatt tagccgtgtt tttatcaaat aaactagaaa ctgagtttaa
    13801 tttaaaactt gatgaagatg agcttggata tatagctatc cactttgctg cttcaaatga
    13861 aagaatgaaa aaaaatacta gtaagaaaat ttgtatagtt tgtcattatg ggataggaac
    13921 aggacaactt atttctgaaa agctaaaaca gaatataagt gatttaagtg ttgtaggtgt
    13981 ttatccagtt agatatctcg atatggctat aagtcaagat gttgatttga tagtatctac
    14041 cgttgaatta aaaggatatg aaaaacctgt cttatatata gaaaatatat ttgatgatag
    14101 cttaattgaa aacgtaaaca aagcctttta tgaaaaggaa gaaagaagaa agataataag
    14161 taacatgttt gatgaaaaag cttttttcag tataaaggct agtactaaag aagaggtcat
    14221 aatttcactt tcaaataaac taaaagaaag agattttata gaggaaagtt ctataaaaag
    14281 tataatagat agagaaaata tttcatcaac tgaaatagga aatctagtag caatacccca
    14341 cacaatagtg aagggagata aaaaatctat aataggagtt ggaatacttg aaaatccaat
    14401 aatttgggat aagcaagagg ttcagctagt atttatggta ttttttaata ctaaagaaaa
    14461 acataatttc tcgatattta aataccttta taattttata aaagatgaag gcggagtaag
    14521 aggaattatt aaaatatgtg actttaataa attaatggca ctgataggta attaaggagg
    14581 aaattaataa tgggaacaaa catatttaac aaagaatatg tatttttaaa tgttgatgct
    14641 aagtcaaagg ttgaagtttt aaaatttata tcaaaaaaag ctaaagactt aaacttggca
    14701 gaagatgaga gattagtata tgaaggttta atggctagag aagaacaatt tactacaaat
    14761 ttaggagaat ctatagcaat acctcacact aaaaatgatg caattgaaaa tccagcagta
    14821 gtagtattga agtttaatga agatgttgtt tggaatgaag gtgaggataa ggttaaatta
    14881 gcaataagct tacttatgcc aggaaaaagt aaagagaata ttcatcttaa attattatca
    14941 tctctttcaa gaaaattaat aaataaagaa tttaaagatt ctcttttaaa aagtgataat
    15001 gttgaagaaa tatctaactt aataaatgaa gctttaggtt tataattaaa cactaaggtt
    15061 ataaaataaa aaaataatta actaaaaata ggaggaattt gaaatgaata aaaaattagt
    15121 agcattatgt gcttgtccaa tgggactagc tcacactttc atggcagcag aagcaataga
    15181 gcaagcagca aaggcattag gttatgaagc aaaagttgaa actcaaggtg ctgatggtgt
    15241 acaaaatgag ttaacaagag atgatatatt aggagcaact atgataatac atgcagtagc
    15301 aataacacca gaaggtatgg aaagatttga tggttgtgaa gtttatgaag ttgaattaca
    15361 agaagctata aaaaatgcag aaggtgttat aaaagaaata gaagaagatt taggaatata
    15421 ataaatttat tgtggaggaa atagaatggc aattaagaaa aaagttataa acagttcagg
    15481 tgcttctaca acaggaggaa ataatgtagg aaaatctact ccatctagtc caactcaatc
    15541 aaagggaaat ggaaaatgga aagaaataag taaacatata atgacaggta tatcatatat
    15601 gataccagtt ttagtaatgg gtggacttat aggggcctta tctcaattaa taccatatgc
    15661 tatattagga cttgacccat ctgtaggtat agttgatgct atgaactcag gagaatttac
    15721 agggttcaag cttagtcttt taaatatagc acaattaatg tcaaatttcg gatttacttt
    15781 gtttggattt gcaataccac tgtttgcagc attttgtgca aactctatag gtggaaaaac
    15841 agctttaatt gcaggattta taggtggata tatagctaat aaacctgtag gtgtaccaca
    15901 attcgttgat ggacaatgga ctgaagttgt tccagttgca tcaggatttt taggtgcaat
    15961 actaatagca tttataatag gatattttgt aaaatggtta aataaatcta taaaagtttc
    16021 tcataactgg ttagctttta aaacaacatt tttaatacca ttaatagcgt cattagcttg
    16081 tatggtattg atgatattta taataactcc atttggtgga ttaattaatg aaagtatgaa
    16141 aaacttctta acagcagcag gagcagctgg agaatatgta tatgcaacag ctttggcagc
    16201 agctacagca ttcgacttag gaggaccaat aaataaagca gcaggattcg ttgcacttgg
    16261 tttaactaca gagaatgtac ttccaataac agcaagaaca atagctatag ttgttcctcc
    16321 aattggacta ggattaacaa cattattaga taaaagatta gtaggaagaa gagtatatga
    16381 tagacaattc tatcaagcag gaaaaacttc tatattctta tcatttatgg gtatatctga
    16441 aggagcaata ccatttgcac ttgagagacc aggttttgtt atacctttaa acatagttgg
    16501 aagtgtaata ggagctatta caggtataat tctaggagca attcaatggt tcccagagtc
    16561 agctatatgg gcatggcctc tagtagataa cttatttgga tatataatag gtatagcagt
    16621 tggtgctata ttcatagcag taggaaatat attctataga aataaattaa taaaagatgg
    16681 taaacttgtt gtagattata tcgactagta tttaattttt actatctata aataactttg
    16741 gctggtaata tggttttaat caatattacc agccttacct atatgattaa ttaaaggcgg
    16801 tggagataag atatggataa gatagaaaaa gtaagagagt attttagaga atatgatata
    16861 gatggatttc ttataaatag tcctacaaac aagttttatg ttggaaattt atttagctct
    16921 tcaggttatg tttttataac taaagaatca caatatataa tagtagattt tagatatttt
    16981 gaagaaatta aaagaaaatc tagtttgttt aatgtagtat taatggacaa aactagaact
    17041 cattttgata taattaatga tatttgtaga gagcagaata taaaggaaat tggatttgag
    17101 ggaaatgaag tatcttttga tttatataga agtatgagta ataagttaag tgcaacctta
    17161 aaatcagttg atttatctac attaagggaa acaaaaaatg aagacgaaat aaaatatata
    17221 aaaaaagctt gtgaaatagt tgatgctaca ttttatcata tagtagactt tataaaggtt
    17281 ggtatgactg aaaagcaagt tgaaaatgaa attgttagaa ttataaaaga tttaggtggt
    17341 caaaaagaat ctttcgatac aatagttgca tctgggctta gaggagcatt acctcatgga
    17401 aaagcaagtg aaaaagttat agagtatgga gattttgtta cctttgactt tggtgctaag
    17461 tataataatt attgttcaga tattaccaga accatttgta tgggtactat aaataaagag
    17521 ttagaagaaa tatataatat agtaagaaaa gctaatgaag agtgtataag ggttttaaga
    17581 ccgggaatga ctactggaga gatagataaa gttgcaagag acataattgg ttcatatgga
    17641 tatgctggta attttggtca taatctgggt catggtgtag gaattatggt tcatgaatat
    17701 cctgcattag ctcctgaatc aaatgaagta ttaaaagaag gaatgattgt aactatagag
    17761 cctggtatat atgttccaag tcttggaggc gtaagaatag aagatgatgt acttataaca
    17821 caagatggat gtatgagatt gactacatct actaaggatt taatagttgt aagctaaaaa
    17881 taaattagat aaatatttta taacaataag aaaagaagag gtaaatttaa atgtataatt
    17941 ttgatgataa accagataga gtaagtgaaa agtgtagaaa atgggattta aatattataa
    18001 gagataaatt tggagatatt agagaagatt ttataccaat gtggattgct gatatggatt
    18061 ttaaaatacc aacagaaata gaaaacaaat ttattgaagc tgtaagaaga ggtgtatttg
    18121 gatatacata ctgttatgat gaattttatg attcagttat tggttggcaa aaaaatatgc
    18181 atgaggtaaa tgtagaaaaa gaatggatta cattaactta tggaactgta tcaacacttc
    18241 attatgtagt acaagctttt tgtaagaaag gtgatagtat aatattaaat acaccagttt
    18301 atgacccctt tgaatcatca gcaaaaaaac aaggtgtaaa tgtaatttgt aatacattag
    18361 atgttgtaaa tgatagatat tatataaatt ttgataaatt agaagttcaa attaaagaaa
    18421 ataagcctaa attaatgatg ttttgtacac cacataatcc atctggaagg atatggacaa
    18481 ttgaagaaat gacaagggta gcaactatat gtaaagaaaa taatgttatt ttggttgcag
    18541 atgaagtaca tgcagagcat attcattatg gtacatttaa ttcaatactt aagattggaa
    18601 aagagctttt agaaaatgtt attctattga catcaccaaa taaaggattt aatcttggtg
    18661 gattaaagac ttcttattca atagttataa ataaggatat aagggacaaa tttagaaata
    18721 aattaaagca aaattctata acttctccaa atgtgtttgg aataatagga ttgataaccg
    18781 cttataatga atgtcatgag tggttaagtg gtgtaaatga atatataaaa tcaaattatg
    18841 aacttttaga aacttgggta aataaatata ataagataaa attgatgaaa atggaatcat
    18901 cttacttagc ttggatggat ataagtggat taggtattag tgcaagtgag tttacagata
    18961 aattggctat agatacagga gtattattag aagatgggag tcattttgta aaagatggtg
    19021 agaagtatgt tagaataaat ttaggaactc aaaaggaaaa tgtaattgaa gctttaaata
    19081 gaatggattt atttttaaag tctttataaa aatttggttg aattagtatt ttatataaat
    19141 ttaaactatt aatatggtat ata
//
LOCUS       DAEMIK010000077        18990 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEMIK010000077 DAEMIK010000000
VERSION     DAEMIK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.077"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..1570
                     /locus_tag="KPK87_002234"
     CDS             167..1570
                     /locus_tag="KPK87_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG2538381.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      1742..1827
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1951..7899
                     /locus_tag="KPK87_002235"
     CDS             1951..7899
                     /locus_tag="KPK87_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG2538382.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNNKDENINQTDNNSNNKDENNDKQQENSDK
                     QEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALETYADV
                     QNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEMNIPKI
                     NKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTIEVRDI
                     EAYGLYRVSIQSLNGDWSSDYKDATPENLDGVPENVDSNYKPIQFSPDSILEFQVVPD
                     TKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIRGTSSK
                     IEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKENGGFGI
                     PTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRGPIITF
                     DKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNNNKYVM
                     VRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVELKEDVT
                     QEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQEINKL
                     GKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYTQYYGE
                     SGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVAGATKI
                     PHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSYGYSEK
                     TGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMDLVYSE
                     RGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHVGVEYG
                     SVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLALLAQT
                     FDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGSMLKNN
                     SDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFKAWGVE
                     ITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDGIQSGS
                     LVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKGNSKAK
                     FTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITNTVSDG
                     DVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEENPYII
                     VDMKETLPIVGLKYTKPEAEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSNDKKNWE
                     DVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIEVAELD
                     IIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSNTSEDETGKIPAGSIVITG
                     EYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKENFDSLS
                     DKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKARKATRN
                     SIDISNKKVITVSNKVKGNIVRDK"
     gene            8109..9989
                     /locus_tag="KPK87_002236"
     CDS             8109..9989
                     /locus_tag="KPK87_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG2538383.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASIKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASQAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIVNRDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            10356..11957
                     /locus_tag="KPK87_002237"
     CDS             10356..11957
                     /locus_tag="KPK87_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG2538384.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSNKTV
                     LEGAKNITSVGEINEKVIQQCISASKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINFYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            12176..13765
                     /locus_tag="KPK87_002238"
     CDS             12176..13765
                     /locus_tag="KPK87_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBG2538385.1"
                     /translation="MKVNKRVLSIGLTISLIMAGATNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDARELANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            13978..16305
                     /gene="slpA"
                     /locus_tag="KPK87_002239"
     CDS             13978..16305
                     /gene="slpA"
                     /locus_tag="KPK87_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG2538386.1"
                     /translation="MNKKNVAIAMSALTVVGSAAPIFADAVPSESNANNYTVVKDKYK
                     DIYDEIEKLVKENDQIISDYKALEGNGAKSDDVIWSELVNKGAVNVIKMKVNEVAGDS
                     NKDSDDIPKVPETTIRTGTVADKKYVEDKLKNLDSDQYVDFSIQLKGTKGESTTKYTN
                     AELTTLANNSGKEILDGIKATTPERLTENGVLSQVAKDAVSGKTEAATAEVLASYFTV
                     SSSLNKVTVSFAEPSTGKVLTTDAANTTVESSGVKNKISAETGYNTIDLTTESNRLDF
                     SKPKFTAGKFSGFEEKAPVDGDVTPGKTYNVRVINAKQSNIKATDYDGIKAIVQKYDF
                     DGAYIDKVYKEVDKLNKEDKLDGSAYDIDGTYKAVFYATGKRLQGFSSYGKNFTKDEP
                     QGGIAVGDAVYKLVIESDDEEDFVDGIEDLKDLNNSYTDVISIAGNDRIETAIELSKT
                     YYNSTDKDALYKNEVNNVVLVGSQAIVDGLVASPLAAEKEGPLLLTSKEKLDSSVKSE
                     IKRVLGLSSTSSINSKKKVYLVGGENSISKDVEKAISDMGVKVERLSGDDRYSTSLKI
                     ADEVGIDNDKAFVVGGTGLADAMSIAPVASQLDSSKDATPIVVVDGKADKLSTDAADF
                     LDGASKVDIIGGVNSVSDKVKDSIKDTIDKAPTRVSGDDRQDTNAQVINEYFKKENKT
                     VKNFFLAKDGSTKEDQLVDALASAAVAGNFGSVNGNQDKVSPAPIVLATDNLSSKQHV
                     EISKVVNDDASNKIVKVGGGIADSVVNKLKDLLGM"
     gene            16484..18829
                     /gene="secA"
                     /locus_tag="KPK87_002240"
     CDS             16484..18829
                     /gene="secA"
                     /locus_tag="KPK87_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG2538387.1"
                     /translation="MSVLDSILDKADEQEIKKLNFIVDKIELLENKMKSLSNEELKNM
                     TNVFKNRLSKGETLDDLLIESFAVVREASKRILGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGRGVHVITVNDYLAERDKELMRPVYEFLGMSVGVIIANQN
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVTNLSNKVQRTLNFAIVDEVDSILIDEAR
                     TPLIIAGNGDEDLKLYKVADSFLKTIKEEDFEMERKDKTIALTESGISRAESFFGIKN
                     LTDIKNIELYHHINQALRAYKLMEKDVDYVIFKGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTFQNFFRLYKKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRGDLHDKVFKTEKEKYEAVVEEIVRIHKTRQPILVGTVSVEKSEKLSNMLKK
                     KNIKHQVLNAKQHDKEAEIISKAGKLEAITIATNMAGRGTDISLGAGDKVEESEVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TISKENRVIESKNLTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYRERNKVL
                     NGEDIQEDIQKMVKDIIQVAADTYLIKRKRDYYGYFKYLYNTFMPNDTLLIPGIDQKS
                     VDEIVSSTYEISKRVYDLKKMMIGIEKVIEMEKIVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEVLNKNIREATIQYLYKFN"
BASE COUNT         7973 a         2080 c         3562 g         5375 t
ORIGIN      
        1 atgttttgaa tttatagaga ataaaaactt taggtatatt gtttgagttg tatggataac
       61 tatggtatca tatataaaat atggtaacaa aattgtaact tttgattaaa aataagtatg
      121 agtactaata aaaaatatga aaagtacatt gggggtagaa aataatatga agaatagtaa
      181 aaagatatta gctataggac ttacactatt tttagtaatg gtaaatactc ctatggtaaa
      241 tgcgttgaca tcagttgagc aaataaaagg gaatgacaga tacgagacag cagcaaaaat
      301 agcagataag caaaactaca atacagcaat actaatcaac tcagataata gcttagcaga
      361 tggtctaagt gcaagtggtt tagctggagc cttaaatgcg cctattttga tgacaaaaca
      421 aaaccagatt ccaaacacaa ctatggaaag attaaacaaa gcaaagactg tgtatataat
      481 aggatcagaa tcaacaataa gtaaaaatgt tgagaatcag ttactatcca aaaagaaggt
      541 tgtacaaaga atctttggtg aaaatagatt tgatacaagt ataaaaatag ctgaaaaaat
      601 caaagaaatt aagccaatag ataaagtaat tatagccaat ggatttacag gagaagcaga
      661 tgcgataagt gcatcaccag tagctgctag agatggagta cctataatac ttacagatgg
      721 aaatagtgtg ggatttgaca cgacaggctt aaagagttat gcgcttggtt ctagtgaaat
      781 aatcagcgac gaattagtta agagcacaaa ttctattaga ttgggtggca cagatagatt
      841 tgaaactaat aagatagtta tacaagagtt ttataaaaat agtaaagaat tttatctaag
      901 taaaggctta caattaacag acgctttggc tgcctctaca atagcaaaaa atgctcctgt
      961 agttttagtt gaaaatggca gtaacaagag tattttaagt ggtgcagaca aattgactgt
     1021 actaggaggc ataaatcaga acgttatcaa gcaatgtata aatcaagctt caccaaatca
     1081 acaagggtta tattacaatc caaatgatag agcatttaaa gagagaataa agggtaaagt
     1141 atatgctctt acaaagcagt atagaaaaga gaatggagta agagcgttat ctgtagcaag
     1201 cagattagag ggtctagcaa atgattggtc taatttaatg gcaaataaga agactttatc
     1261 tcatacgata aatggaaaaa attcatattc tacttttttg aaatacttag attggagtga
     1321 gattaaacct ggatatatag cagttcaagg tgaaaatata attaaatata aaattccaga
     1381 taaacctgta tatacaaata gagatgcaga tgatatagga aactttattt ttaatgagtg
     1441 gaagacaaat ccagaagaag gcactaatat gttgcataaa gggtatgaaa taatgggatt
     1501 tggtatagct ataacaggag ataagaatct atatgctaca catgaatttt atggaagata
     1561 taaagaataa gaaaatatac acaaaattat gcttgtaagg aagctatata aataattaaa
     1621 ttataatgga aaataacatt tatattaata aattaatata aatgttattt ttttataatt
     1681 taatgtaaaa aaattgtatt aaaaagttta aatatagtat aaaatgggta agaaagtagt
     1741 gaaaattaaa tggcaaaatt agggaaatct aatgacgcaa aactataggg attaaagctt
     1801 ttaaagccat atcagccagt tgctaaaaag agtaaagtct ttttatttta taattaagat
     1861 tctctttgag tgtctttttt tattgtctga aacttatagt atttaaattc atgaaaaatt
     1921 agatatttat taagggagga ttgttatttc atgaaaaagg caatatcttg tgtactagca
     1981 gtatctatgt gtagtgcacc actaaacgtt tttgcagaac ctatattaga ggggaagcta
     2041 agggcagttg aaaaatcagt tacagaaaag ttaaggggga gtcttgaagt agatttaaat
     2101 ttttcactgc caataaaaaa tagcgagtca gaaagtatga ctaatatgca gttacgatta
     2161 aaagatgata gtaacaatag tggaataata aagcttggag aaaaagattc aggaacagta
     2221 aatgttggga cagataatat agaatatact ataaaaaaat tatctgcaag tagaagtgaa
     2281 ataaaaaata aagatgagaa tgtatcatat tataatattg tatttaataa tttacctgtt
     2341 ggaaaataca atgtagaagt ctctggagct ggatttaaga acaaagaaat taaagacatt
     2401 gatatatcaa gttattcaca aagagtctta ttaagcaata gagcaagtgt agataataaa
     2461 aataaacctt taaattatga attattctta atgggtgatg taaatggaga cggtaaagta
     2521 gataagagtg attataataa ggttttagag aatatagatt caaataaaag agaatttgat
     2581 ttaaatagag atggaaaggt agatattgtt gacttagatt atgtacagaa aaatttagga
     2641 caaagtgaag atgcaaaaag cctagaaagt atagtatcta ccaatcctat agtagataca
     2701 agtaaggttg aattaaaagg aaatagtgat gtagatatta atggtaatgt agaaaattta
     2761 tttagtggag aaaataatgg agttacaata tcttcaaaaa atgatgaaat atctgaggaa
     2821 aaaccagcag taatggatat agctttatca gaagcactta atatggaagt catcaatata
     2881 aaagctccag aaggtactgc tccagcaaaa ggaatagttg aggttactga caaagatggt
     2941 aatgttcaac agattcctta tgaaaacaaa gaatatatga aaaacttaaa agatgaggag
     3001 caacaagagg aagtaaataa cgaacaagaa gaagtaaata ataaagatga aaatataaac
     3061 cagactgata ataactcaaa taataaagat gaaaataatg ataaacaaca ggaaaatagc
     3121 gataaacaag aagcaaactc taataccaat caagataaaa ataatgataa agaaaattca
     3181 tcaatggata aacctaattc agatgaagat actccaaaag aagagggtaa tatcagtaaa
     3241 gatgataact ctacaacatc tacatatagt aggtcagcat tagaaacata tgcagatgtt
     3301 caaaatagtg atatagtaat aaatcttggt aaacaagtag cagtaaaaaa gataactata
     3361 aaaataacag ctacaacagc aaacgataga aatcttgcag aaatatcaaa agttgaattt
     3421 ttaaacaatg tatataaaga aataccaaaa ccagagatga atatacctaa aataaataaa
     3481 atatcgacta gcactgctgt tggaaatgaa aaagtatctt tttattggaa taatgaagta
     3541 aatgtatcag catacgaact tatactagac aaaattgata gtaagggaaa agtattatct
     3601 actaaaaaat tacaaactag taaaaataca atagaagtta gagatataga agcatatgga
     3661 ctatatagag ttagcataca atctttaaat ggagattggt ctagtgatta taaagatgca
     3721 acaccagaaa acttagacgg agtaccagaa aatgtagatt caaactataa acctatacaa
     3781 tttagcccag atagtatatt agaatttcaa gttgttccag acacaaaacc agaaccacca
     3841 gaaggtataa atgttaaagg taaatttaaa tctcttgatg taagctggaa aaatcataaa
     3901 caagctaaag attttgactt gtactataaa gaaaaaggtt ctaatgggtc ttggataaaa
     3961 tacaatgata agcttattcg tgggacaagt tcaaagatag aaggtcttaa ggataataca
     4021 acatatgaag ttagaatgac agctactaac cacttgggaa ctagtggaat gtctaaaaca
     4081 tatataggta caactgaaag catggatgca ccaacaacac ctaactatag attaattaat
     4141 actcctaaag agaatggtgg atttggaata ccaacaaatc acattgtaga tgttacttat
     4201 ccagcaggat atgacaaaag tgaatatagt gataaaaatc catttgataa attcaatgta
     4261 gtagatgggg actttacaac acattggaca ttcccaacat ataatgcagc tgagggaacg
     4321 aatagaggac caataataac ttttgataaa tcttatacta tggatacttt tatggctaca
     4381 cctcgttttg atgtttctga taagacaata aatgactttg aggtacgata ttgggatgaa
     4441 aatggtcaat tacacaatct gggggatgtt ccttttgaaa caaagacaga ccctgtaaat
     4501 aataataagt atgtaatggt tagattaaaa gaaccaataa ctgctaccaa gatacaagta
     4561 agtgtttgtt catcctattc atataagtct aatatatcag aacttaagtt ttatgagtat
     4621 gacagtatag aagatgatgt aagaaatcta tttatggatg acctacaagt tgaattaaaa
     4681 gaagatgtaa ctcaagaaaa aataactgaa ttaaaagata gattaaatac tccagataaa
     4741 gctagtggag aatatcatcc atataagaca gttattgaga gagaattaaa attagctcaa
     4801 gacttatata atgatagagc cacacttact gatgtaacga ctgtaaatca agaaataaat
     4861 aaattaggaa aatataaaga taaaaacgga cagtatactg taaattccaa taatttaggt
     4921 atgcaaaatg actggcaggc attaggagta gctgctagag caggagatga aatcacagta
     4981 tatgttggaa gtaaaagtgg aaaaactcct aagttgatat atacacagta ctatggagaa
     5041 tctggtgcat ataagagtgg agaaataaat cttaacactg gaaaaaatgt aattcaatta
     5101 agcaaacttc atagtttaga tatagaatgt ggaggaagtt tatacataag atatgctgaa
     5161 gatactccaa gtggaggaga tataaaagta cgtgtagcag gagctactaa gataccacat
     5221 cttaatctaa atggaacaat aaatgataag agtcctgcgg gagtttctga atctaagaaa
     5281 aagataaaag catatataga agaattagct aaatataaag atgctgtaaa aggagaacca
     5341 tattatccag gaacaaatgt tggtaattca tatggatatt cagaaaaaac tggtgtgtta
     5401 aacactacag atattgaaag tgataaagta acattaaatg ttcctgcaac agctgtatat
     5461 caagggatat caaaaggaaa tgctgattta aatacacaag tagatagatt atataatagt
     5521 atgcttgcat gggaacaaat aatggattta gtttactcag aaagaggagt atttaaaaca
     5581 caagatttag atggtaatgg aactgtagat gataatgaat ttaacttaac taaaaatgat
     5641 atggctccaa aatctcgtat gaacataaaa taccaaagaa tgtttattgg agcatttatg
     5701 tatgcttctg gattacatgt aggagtagaa tatggttcag ttccagggct tatgaatgga
     5761 gtacctttcc aaatagatga tagtggaaaa gctacaggag gaaacttatt tggatgggga
     5821 ataggtcatg agataggaca tgttacagat attggaaaga tgacatatag cgaaactagt
     5881 aacaatgtac ttgctttact tgctcagacc tttgatgata agacacattc aagacttgaa
     5941 ggttcaacaa tggataagat atatgagcat gtaacttcaa atagcttagg tataccaagc
     6001 aatgtatttg aaaggcttgg aatgttatgg caattacatc tagcttatga tgatgatttt
     6061 acgggttcaa tgttaaagaa taactcagat gctgatttaa gtaatgatac attttatgca
     6121 aagatttcaa gaaaatacag agcactttca agtagtgacc caattaatag tttaccaaaa
     6181 gaccaaatgc tagtagcaat ggcatcaagt gttgttgaaa aagatttaag agattacttt
     6241 aaggcatggg gagtagaaat tacaccagaa ttaaacagta taatggatag taagaactac
     6301 gaaaaagaat caagaaagat acaatatgta aatgacgaag ctagaagaaa gattttaaat
     6361 gaaaatatag ctagtatggc aaaagatact aaagtaagtg ctagtttttc tgatggaata
     6421 caaagtggtt ctctagttaa atctaataaa atatctatag acctagatgt agataaggat
     6481 aaagataaga tactaggata cgaaataata agaagtgatg gaaattattt agatggtgaa
     6541 aatggaacac aggtaaaata cagaacagtt ggatttgtgg atgcaaaagg aaatagtaag
     6601 gctaaattta ctgataatat atctcctctt aataacagag catttacata taaggtagtt
     6661 gcatatgatt atcacctaaa tccaacagaa gaatttgaag ttggcactgt aaaattatca
     6721 gatgaaggga aaataaataa atcagcatgg tcattcataa caaatactgt ttctgacggt
     6781 gatgttagaa ctgaaaatga ctcacatgga ccattacaaa atcctgaaat tgataatata
     6841 aaagataatg atgcaagtac tacatataaa ggaaagataa taagtaaagc agagtggaac
     6901 aaagaccctc aaaaagaccc tgatataaat gtagaagaaa atccatatat aatagtagac
     6961 atgaaagaaa cattacctat agtaggacta aaatatacaa aaccagaggc agaagctagg
     7021 aagttctcat taaaaggatt atttaacttt agaaagaata ctagcacaac atacaatcct
     7081 ttaactaatt acaagataca agtaagtaat gataaaaaga actgggaaga tgtaagtcaa
     7141 ggaacttttg aatatggaca ggatttagtt ggtggaggaa aagaccaaga taatgaagct
     7201 agagttacat ttaataaaga tggtaaactt tggacatacc aagctagata tgtaaaacta
     7261 atctcaaata ataagtctaa tatagaagtt gcagaattag atataatagg acctccaggt
     7321 gataatatag aaattggcac aatgaataat gcagacgaaa ctcgtactaa tggaattgga
     7381 aaattagcag aagattatgt atatcaaagt gatgactcta acacaagtga agatgaaaca
     7441 ggtaagattc cagcaggttc aatagttatt acaggtgaat acagtggaaa tccagcattt
     7501 aatttaccac tacttataga taaaaacaat aagactatat ctggagaagc acttttattt
     7561 gcagaagttc cagaaaaagg tgaactaggt gaaatcagta aaggtacatg ggtttactat
     7621 atgccaaaag aaaactttga ttctttaagt gataaggtta aggctgaact atatagatac
     7681 aatgaccttc aaggagatac tccagtaggt caaagatttg taagtgatac aatgtatgta
     7741 cctgtaggag ctaagaatta tgatgagtta aagacaataa gtcttactga tagtaatagt
     7801 aaagctagaa aagctacaag aaattctatt gatatcagta ataagaaggt tataactgta
     7861 agcaataaag taaagggtaa tattgttaga gataaatagt aggtggaact ctaaaaacgc
     7921 aaaaatacta acactaacat tgtatatcta ataagattag gtactagaat agttttaaaa
     7981 tagtagatat taaaagtaaa gtatataaat gtaaccataa atttagggta atttataaat
     8041 taaatataaa agctaggtgg ataagtaaat ttatccactt agttaatgat agataaggac
     8101 gggtgttaat gagtaaaaga aaatctttta taagaactat agcagtatca actatggcag
     8161 tagctgtaac tggtagtgct acatgtgcat atgcagctcc agttttacaa ggaacaaaaa
     8221 cgtatgagaa agtaaatact atagatatat ctgtggactc agtagaaaac atagtatatt
     8281 catttcaagc cagtataaaa gtacaaggtg aggttgaagt agtagataat gagcagaaag
     8341 aaaagattac ttggtctgat aatataaaat ctcagataaa aagtggaaat gcaaatgcta
     8401 cctgtagagc agaatacaac aaatcaagta atacaactac attagatata tacgttacat
     8461 caaatgaaga tttactagac ggaaatagat tgaatatagg aagaatatct gtaaaaaaat
     8521 ctggcagtaa ctcaaatgct gactataaag ttttaggaaa aggaacaagt gataaaccag
     8581 ctttaaaaat tgtaacatat aacaacaaaa cagtggatta tgaaaatata agctctgatg
     8641 aaggattaat atttaccctt attaatgaga gtgaagtaaa accaattggt ggaactggtt
     8701 ctagtaaaaa tgaccctgaa aagtataagg tagagaaaag tgaagcactt gaatatttat
     8761 taaacaatat taggataaac tattctattg ttagtaaaga aacacaagaa agtggttcaa
     8821 atgtaatatt aaaacttgga ttagcacaaa aaacaactaa gggaagaaaa gctacaataa
     8881 acaaatatgt tgaagttaca cttccaaaaa gtttagaata catagttgaa aatgaattat
     8941 caaaaccaga tgaattacca ccagataatg gcaatggtgg aaataatggc ggaggaagta
     9001 attcaggcgg ctcatctagt ggtggaagct ctggaggagg aaattcaagt gattctacat
     9061 ctaatgtcac agtcaaaaaa ttaaaaggtg ctgatagatt tgaaacagct atcaaaatat
     9121 ctcaaagtgg ttggacaaaa tcagatacag ttgtaatagt aaatggagaa gataaaagta
     9181 tggtggatgg gcttacagct acaccacttg ctagtataaa aaactcaccg attcttttat
     9241 catcaaatga aaagcttcct caaaaaactg tagaagaatt gaaaaggcta aatccatcaa
     9301 aagtagttgt tataggtgga aacaactcaa tgccaaattc agttgttgaa gctatcaaag
     9361 ctgtaaattc aaaaatatca gtacaaagaa taggtggaga tacaagatat caaacttcta
     9421 ttaatatagc aaaagaaata gataagacga ataatgtaag caagttgtat ataggagcag
     9481 gaaatggaga agctgattca ttatcaatag catcacaggc tggtaaagaa aaaactccaa
     9541 tagtacttac acaaaaagat ggtgtagata atgaagctga acaatttatt aaatcaaata
     9601 aggtgtcaaa tatatatttt attggtggag tagaaaaaat atctaacaag gctattgaac
     9661 aagttggaaa aatagtaaac agagatatat caaacaatag ggtggcaggt caaactagac
     9721 aagagacgaa tgccaaagta atagataaat tctactctca atctaagcta gatggagttg
     9781 tagttgcaaa tcaagataaa cttatagatg ctttagcagt tggaccatta gcagctaaaa
     9841 acaattcacc agttatactt gctacaaata cgttagataa atcacaagaa tcgtcattaa
     9901 aaggtaaaaa ttcatcaaag ttgtttgaag taggtggagg aatagcctca tctgtaatag
     9961 acaagattaa gagtttaatt gaaaaataaa tattaagtaa atagaaaata agaatgtatt
    10021 tagatataaa gttatatatt taaatgcatt cttattttat tttcattatc ttaacaatct
    10081 aataatattg aggttaaaat gtataataat ttgagctata ttaagtttaa ttttaaataa
    10141 agcagatatc ttttatatat ataaatataa attcaatttt gaatatgttt aaaaagaagt
    10201 taaaaataat tttttaaatt taataaaata aaacactaat atatagacga gatatattca
    10261 tatcatatat actctataat atatggaaaa taaagagaaa ataaatggaa aataatcaca
    10321 atataaatat aaaatataaa aatcatcagg gggatatgaa aatgaacaaa aaaatattat
    10381 cattaggtct agcagtatca ttaatcttag taaacttcaa aagtgtaaat gcatcatcag
    10441 tagtagaaaa aatatacggt aaagatagat atgaaacagc agcaaagata gctgataaac
    10501 agacttatga aacagtaatt ttagtaaata cagaaaaatc acttgcagat ggattaagtg
    10561 caagtggatt atcaggagct acaaaagctc caatactatt tacacaacaa aataagatac
    10621 cagcagacac aaatagatgt ctaaaaaata tcaaaaaagc atatataata ggtacagaag
    10681 atactataag taaatcagta gaaaaagaac tagattctaa aaatatagaa gtcaaaagaa
    10741 ttggtggaga agatagacta aaaacaagct atttaatagc taaagaaata gcaactataa
    10801 aaaaagtgga taaagtacta ctaactaatg catatagtgg agaagcagat gcaatgagtg
    10861 tatcatcagt agctactaga gatggagctc caatcatact tacagatgga aagagtgtgc
    10921 cttttgatgt aaaaaatatt caatcatatt gtataggttc agaggagata atgagtaatc
    10981 ctctagttaa aaatacaaac tcagtaagaa tagaaggaac tgaccgattt gaaactaaca
    11041 aaaacgtaat agactacttt tttaatagtg cagatggatt ctatgtatca gatggatacc
    11101 aattagtaga tgcaatagca gctgcaccac ttactaaaaa ctctccaatg gtactagtaa
    11161 atgatggaag caataaaact gtattagaag gagctaagaa tataacctct gtaggtgaaa
    11221 taaatgagaa ggtaatacaa cagtgtataa gtgcttcaaa gtcaaatgga caaccaccaa
    11281 caattacagt tggaagtaca gaggtatata agggtgaaaa gtttgacact ggcaagttaa
    11341 atatagtagc taaagataac acagggaagg tattaccaat agaagttgat gggtttatag
    11401 atacaaatag agtaggtaca tacatattga cattaaaagc tacagacgaa tggggaaaaa
    11461 gcacaggaaa aagagtagaa ataaaagtgt tggatgacaa atctcatgat tacaacagcc
    11521 cagaatttaa aaaaatggta tctactgaaa tgtataactt aatcaatttc tatagaaaag
    11581 aaaaaggcaa agaacctttg gtagtatcta gtaggctaga aggtatggca aatgcatggt
    11641 ctaagtatat gatggataaa aaagtatttg cacattatat agatggaaaa aatgctccac
    11701 aagtattttc tgaatttgga atgagaagtg aggaaaatat agcatatatt tatatagatt
    11761 ctaagaacgt tcaaactaca caagatgcaa aagatttggc aaaggctata tttgaggtgt
    11821 ggaagaaatc tccagagtac aatgcaaaca tgcttagtaa tgagttttat agtactggat
    11881 ttgggcttta tatattatct gatggtcaag tgcatgctac tcaggagttt ttaaatggca
    11941 atgaaggtag cttgtaaaaa attccataaa tcttgttttt ttgttaattt actgatatta
    12001 taaaatgaaa atcacatcta aagtactttt tgtaatctgt tttgttattg atttacaggt
    12061 tatggtattt taggtgtttt ttgttttagt agtttataaa ttttgaggga atcactactg
    12121 aggatgtaat ttatgattag ctgaattata taaaaaaatt agtaggagga gtttgatgaa
    12181 ggtaaataag agagtattat caataggatt gacaatatca ttaataatgg caggagcaac
    12241 aaatatcaat gcactttcga gcatagaaaa gatacaaggg aaagatagat atgagacagc
    12301 tgccaaaatt gcacagaaac aaacatatga aaatgtagta ttagtaaata cagataatac
    12361 actagctgat ggattaagtg caagtggatt agcaggaaca gttaaagcac ctatattatt
    12421 atcacaaaga aacagtatac catcagacac agaaaaaatg ttaaaagatg taaagaaggt
    12481 ttatataata ggaactgaag attcaatagg aaaatcagtt gaaaatgaat taaagcaaaa
    12541 aggaatagaa gttaaaagaa taggtggtaa tgatagaatt gaaacaagtt atcttatagc
    12601 aaaagaaata gcttcaataa aacctataga taaggttttt ataactaatg gttatacagg
    12661 tgaagcagat gctatgagtg catcatcagt agcatcaaga gatggagcac ctataatttt
    12721 aactaatggt aaaaatgttc catttgagaa aaaagaaggc gtgcaatgtt atgctcttgg
    12781 ttcagaggag ataataagta atgatttagt taaaaaaact aatgcagtta gattagctgg
    12841 agaagataga tttgagacta ataaaaaagt tataaaacat ttttatagtt cggcaaaaga
    12901 attttatctt tctaaaggtt atcaattagt agatgcagtt gctggttctt ctatagctaa
    12961 aaatgcacct attgtattgg ttgatggaaa tagtgataag tctgtactta gaagtgctga
    13021 taagattaca gcactaggag gaatagatga aaagacacta gaacaatgtt taagtgcatc
    13081 atcactggat gcaagtgcac caacaataac tgttggaaat ttaaatattt atcaaggtga
    13141 taagtttgat ataagtaaac ttaatatagt agcaaaggat agcaatggta acgatttgac
    13201 accagagctt ataggaaata ttaatactga taaggtgggt aaatacaaag ttactataaa
    13261 agctactgat attggtggaa agactacctc aataagtgta gaagtaaatg tacttgaata
    13321 taagactaat gatatgaata gtagtgagtt taaaagaatg gtttctagtg aaatgtataa
    13381 tttggtaaat tcatatagaa aagaaaaagg gaaagagcca ttacaagttt ctaaaaactt
    13441 agaagggctt tctaattcct ggtctaagta catggcagat aaaggccaat tttcacatgt
    13501 aatagatggt aaaaaatctg ttgaagtatt tactggatat gggttgagaa gtgaagaaaa
    13561 tatagctttt gtaccacttg ttacaaagag cacttatacg acaaaagatg ctagagaact
    13621 agcaaatgta atatttacag tttggaaaaa atcggataaa tataatgaaa atatgctaaa
    13681 cagtgatttt gtatatactg ggtttggatt gtatatattg cctaatggag aagtgtatgc
    13741 tactcaagaa tttttaaata agtaaaatta aattttttaa gttattaaat attgaaattt
    13801 aagggtataa aaactcatat gcttgggaaa aataataatt tcaaaaaaat gacttgcaaa
    13861 aagaataaaa atggattatt atagagatgt gagaaatatt aggaatatat ggatgattat
    13921 tctatgtaca taataaagag atgtaatttt aatataatgt tgggaggaat ttaagaaatg
    13981 aataagaaaa atgtagcaat agctatgtca gcattaacag tagttggttc ggccgctcct
    14041 atatttgctg atgcagtacc aagtgaaagt aatgctaaca attatacagt ggtaaaggac
    14101 aaatataaag atatttatga tgaaattgaa aagttagtta aggaaaatga tcaaataatt
    14161 tctgattata aagcactaga gggcaatgga gcaaaaagtg atgatgtgat ttggtcagaa
    14221 ttagttaaca agggtgctgt caatgtaatc aagatgaaag ttaatgaagt tgctggagat
    14281 tcaaataagg attcagatga tatccctaaa gttcctgaaa ctactataag aacaggaaca
    14341 gttgcagata aaaagtatgt tgaagataaa ttgaaaaatt tagattcaga tcaatatgtt
    14401 gatttttcaa tacaacttaa aggaactaaa ggagaaagca ctacaaaata tacaaatgct
    14461 gagttaacta cattagctaa taattctggt aaagaaatac ttgatggaat caaagcaact
    14521 acacctgaga gattaactga aaatggagta cttagccaag tagctaagga tgctgttagt
    14581 ggaaaaacag aagcagcaac agcagaagtt ttagctagtt attttactgt tagttcatca
    14641 cttaataagg ttactgtaag ttttgcagaa ccttccacag gaaaggttct tactacagat
    14701 gcagcaaata ctactgtaga gtcttctggt gttaaaaata aaataagtgc agaaactgga
    14761 tataatacaa tagatttaac aacagaaagt aatagattag attttagcaa acctaagttt
    14821 acagctggaa aattttcagg atttgaggag aaggctccag ttgatggtga tgtaacacca
    14881 ggaaaaacat ataatgtaag agttataaat gcaaaacaat ctaatataaa agcaactgat
    14941 tatgatggta taaaagctat agtacaaaaa tatgattttg atggagctta tatagataaa
    15001 gtatataaag aagttgataa attaaacaaa gaagataaac ttgatggtag tgcatatgat
    15061 atagatggta cttataaagc tgtattctat gctactggaa agagattaca aggattttca
    15121 agttatggaa agaacttcac taaagatgaa cctcaaggtg gaatagctgt tggtgatgct
    15181 gtttataaat tagttataga atctgatgat gaagaagatt ttgtagatgg aatagaagat
    15241 ttaaaagatt taaacaatag ttatactgat gttatttcta tagcaggaaa tgatagaata
    15301 gaaactgcaa ttgaattaag taaaacttat tataatagta cagataagga tgctctttat
    15361 aaaaatgaag ttaacaatgt tgtattagtt ggttcacaag ctatagttga tggattagtt
    15421 gcctctcctt tagcagctga gaaagaagga ccattactat taacttcaaa agaaaaatta
    15481 gattctagtg ttaaatcaga aataaagaga gtattaggat tatctagcac ttcttctata
    15541 aattctaaaa agaaagttta tttagtagga ggagaaaatt ctatatctaa agatgttgaa
    15601 aaagcaatat ctgatatggg tgtaaaagtt gaaagattat ctggagatga tagatattct
    15661 acttctttaa aaatagctga tgaagttgga atagataacg ataaagcatt tgtagttgga
    15721 ggaacaggtc ttgcagatgc tatgagtata gcaccagtag cttctcaatt agattctagt
    15781 aaagatgcta ctccaatagt tgttgttgat ggaaaagctg ataaattatc tactgatgca
    15841 gctgatttct tagacggagc ttctaaagta gatataatag gtggagtaaa ttctgtatct
    15901 gacaaagtta aagattctat aaaagatact atagataaag ctccaactag agttagtggt
    15961 gatgatagac aagacactaa tgctcaagtt ataaatgaat acttcaaaaa agaaaataaa
    16021 actgttaaga atttcttctt agctaaggat ggctcaacta aagaagatca actagtagat
    16081 gcattagctt cagcagcagt tgcaggtaat tttggaagtg ttaatggtaa tcaagataag
    16141 gtttctccag ctccaatcgt tttagctact gacaacttgt cttctaagca acatgttgaa
    16201 ataagcaaag tagtaaatga tgatgcttct aataagatag taaaagttgg tggaggaatt
    16261 gctgattcag tagttaacaa attaaaagac ttattaggaa tgtaattaat aaattattat
    16321 ttatataaaa aagaatggat gtatatccat tcttttttgt tatcatattt actttttatc
    16381 taaagaaaac ctttcattaa atttcaatat aaaaataaat gtctactatt atctaaaatt
    16441 aatgtataat attagttgta tgaaaaaaaa ggaggtatgg tatatgtcag tattagattc
    16501 aatacttgat aaagcagatg aacaagaaat aaaaaaatta aactttatag tagataaaat
    16561 tgaattatta gaaaataaaa tgaagtctct atcaaatgaa gaattaaaga atatgactaa
    16621 tgtatttaaa aatagattga gtaaaggaga aacattagat gatctgttaa ttgaatcatt
    16681 tgcagttgta agagaggcat cgaaaagaat attaggtatg cgacaatata gggtgcaatt
    16741 gattggtgga atagtaattc atcaaggaaa aattgcagaa atgaaaactg gagaaggaaa
    16801 aactttagtg gaagtagcac cagtatatct aaatgcactt acgggtagag gagtacatgt
    16861 aataacagtg aatgattact tggcagagcg tgataaagaa cttatgcgcc cagtttatga
    16921 attccttgga atgagtgtag gtgtaattat agctaatcaa aatccaagta taagaaaaga
    16981 gcaatataaa tgtgatatta cttatggtac aaatagtgaa tttggatttg attatttaag
    17041 agacaatatg gttactaact tatctaataa agtacaaaga actttaaact ttgcaatagt
    17101 ggacgaggta gattcaattt tgatagatga agctagaact ccacttatta ttgcagggaa
    17161 tggagatgaa gatttaaagc tctataaagt agctgatagt tttttaaaaa cgataaaaga
    17221 agaagatttt gaaatggaaa gaaaagataa gactatagcg ttgacagaaa gtggtataag
    17281 tagagctgag tcattttttg gaataaaaaa tcttacagat ataaaaaata tagaacttta
    17341 tcatcatata aatcaagctt taagagctta caaattaatg gagaaagatg ttgattatgt
    17401 catttttaaa ggtgaagtaa tgatcgttga tgaatttaca ggaagagtaa tggatggaag
    17461 acgatataca gatggtcttc atcaagctat agaagcaaaa gaaggtgtag agataaaaaa
    17521 tgagtctaaa actatggcta ctgttacctt ccaaaacttt tttagattgt ataaaaaact
    17581 ttctggaatg acaggtacag ctaaaactga agaaggagag tttgagtcta tttataaact
    17641 taatgttgtg caaataccta ctaataaacc agttattaga ggagatttac atgataaagt
    17701 atttaaaaca gagaaagaaa agtatgaagc tgttgtagaa gagatagtaa gaattcataa
    17761 aaccagacaa ccaatacttg tggggaccgt ttctgttgaa aaatctgaaa aattatctaa
    17821 catgctcaag aaaaaaaata taaaacatca agttttaaat gccaagcaac atgataaaga
    17881 agctgaaata atatctaaag caggaaaact agaagccata acaattgcaa ctaatatggc
    17941 tggacgagga acagatattt ctctaggagc aggagataag gtagaagagt cagaagttaa
    18001 gaatttaggt ggactttatg tcatagggac agaaagacat gagtctagaa gaattgacaa
    18061 tcaacttaga ggtcgttctg gtcgacaagg agatcctggg acatcaagat tttttgtaag
    18121 tttagaagat gatgtaataa aactttatgg tggtaaaaca atagaaaaac ttatgaagag
    18181 aacaatttcc aaagaaaata gagttataga aagtaaaaat cttacaagag ctatagaaag
    18241 agcacaaaag ggagtggaag gtaaaaactt tgagataaga aaaaatgttc ttaaatatga
    18301 tgatacaatt aatgaacaaa gaaaagttat atatagagaa agaaataagg tactaaatgg
    18361 tgaggatata caagaagata ttcaaaaaat ggttaaagat attatacaag tggcagcaga
    18421 tacttattta ataaaaagaa aaagagatta ttatggttac tttaaatacc tatataatac
    18481 atttatgcct aatgatactc ttttaattcc tggcattgat cagaaaagtg ttgatgaaat
    18541 agttagtagc acatatgaaa tttcaaaaag agtttatgat ttaaaaaaga tgatgattgg
    18601 tatagaaaaa gttattgaaa tggagaaaat tgtcctttta aaagttgttg atcaatattg
    18661 gatagatcat atagatgcta tggaacagtt gagacaatat ataggattaa aatcttatgc
    18721 acaaaaagat ccatttaagg aatacgcttt agaaggttat gatatgtttg aagttttaaa
    18781 taaaaatata agagaagcaa ctatacaata tttatataaa tttaattaga aatggattat
    18841 atttatttta tgttgaaata aaatgtaaaa tatagtaata tattttttta taaatgaaaa
    18901 agaagctgat tgacagcttc tttttctatg tgagaaatac ggaaaaaaga gatgcttgaa
    18961 atttctttct tccatttaat attatactat
//
LOCUS       DAEMIK010000078        18924 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEMIK010000078 DAEMIK010000000
VERSION     DAEMIK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.078"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            67..1518
                     /locus_tag="KPK87_002241"
     CDS             67..1518
                     /locus_tag="KPK87_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG2538388.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1738..2199)
                     /locus_tag="KPK87_002242"
     CDS             complement(1738..2199)
                     /locus_tag="KPK87_002242"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG2538389.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2304..3497)
                     /locus_tag="KPK87_002243"
     CDS             complement(2304..3497)
                     /locus_tag="KPK87_002243"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG2538390.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDIYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3520..4185)
                     /locus_tag="KPK87_002244"
     CDS             complement(3520..4185)
                     /locus_tag="KPK87_002244"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG2538391.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4219..5331)
                     /gene="ribD"
                     /locus_tag="KPK87_002245"
     CDS             complement(4219..5331)
                     /gene="ribD"
                     /locus_tag="KPK87_002245"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG2538392.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5463..5584)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5773..8256
                     /gene="recQ"
                     /locus_tag="KPK87_002246"
     CDS             5773..8256
                     /gene="recQ"
                     /locus_tag="KPK87_002246"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG2538393.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8345..8456
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8598..9899
                     /gene="thiC"
                     /locus_tag="KPK87_002247"
     CDS             8598..9899
                     /gene="thiC"
                     /locus_tag="KPK87_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG2538394.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDVVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9953..10147
                     /gene="thiS"
                     /locus_tag="KPK87_002248"
     CDS             9953..10147
                     /gene="thiS"
                     /locus_tag="KPK87_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG2538395.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNEYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10140..10943
                     /gene="thiF"
                     /locus_tag="KPK87_002249"
     CDS             10140..10943
                     /gene="thiF"
                     /locus_tag="KPK87_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG2538396.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDIIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11006..11776
                     /locus_tag="KPK87_002250"
     CDS             11006..11776
                     /locus_tag="KPK87_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG2538397.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11793..12896
                     /gene="thiH"
                     /locus_tag="KPK87_002251"
     CDS             11793..12896
                     /gene="thiH"
                     /locus_tag="KPK87_002251"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG2538398.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12897..13586
                     /locus_tag="KPK87_002252"
     CDS             12897..13586
                     /locus_tag="KPK87_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG2538399.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLVRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13774..15168
                     /gene="yfcC"
                     /locus_tag="KPK87_002253"
     CDS             13774..15168
                     /gene="yfcC"
                     /locus_tag="KPK87_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG2538400.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15685..17052
                     /locus_tag="KPK87_002254"
     CDS             15685..17052
                     /locus_tag="KPK87_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG2538401.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17077..17685
                     /locus_tag="KPK87_002255"
     CDS             17077..17685
                     /locus_tag="KPK87_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG2538402.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            17733..18104
                     /locus_tag="KPK87_002256"
     CDS             17733..18104
                     /locus_tag="KPK87_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG2538403.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18283..18621
                     /locus_tag="KPK87_002257"
     CDS             18283..18621
                     /locus_tag="KPK87_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG2538404.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT         7202 a         2087 c         3217 g         6418 t
ORIGIN      
        1 ttattcattt ttttgatttt aagatttatt gagaaaacaa aactaaatag gggggtaatt
       61 aatataatgg attttttcaa tggtatttta acgagtatta acaatgcagt agtatatgta
      121 aacaattact tatggtcgta tatcttaatt ataatgttag ttgtaattgg tatttacttt
      181 actattaaaa caaattttgt acaattcaga tattttatag aaatgtttag acttctgggt
      241 gatggtacag cgaataaaaa tgcaaaaaag gaaggaaaaa tctcttcatt tcaagcattt
      301 tgtataagta catcttcaag agttggaact ggaaatatag ctggaatagc aattgcagtg
      361 gtagcaggag gacctggtgc tgtattttgg atgtggctta tagcattaat tggttctgca
      421 tcaagctttg ttgagagtac attagctcag atttacaaag tgaaagatgg tcaagctttt
      481 agaggtggac cagcgtatta tatggaacaa ggtctaaata aaaaatggat gggtatttta
      541 ttctcaatac ttataactat atgttatggt tttgtattta atgcagttca agcaaatact
      601 gtttcacttg catttaataa tgcatttgga ataagtaaaa tggctatggg aataggtctt
      661 ggaattttaa ctgcaatagt tatttttggt ggagtgcata gagttgcaaa agtttctgaa
      721 ataatagttc ctgtatttgc aggattatat atattagttg ctttggctat agttgtaatg
      781 aatataacag agataccaag tgttatagca ttgatagttg aaagtgcatt tgattttaaa
      841 ggaatggcga ttggtacatt tatgggagtt gtaatgactg gagtgaagag aggtctattt
      901 tctaatgaag caggtatggg tagtgctcct aatgctgctg caacagcaca tgtaactcat
      961 cctgtaaaac aaggtttaat tcaatcttta ggtgttttta cagatactat aataatatgt
     1021 agttgtacag cattcatggt attattatat tcaggttata gtagtagtgg agcaactgga
     1081 attgaattag ctcaagaagc tttaactcat catataggac cagttggaaa tatatttata
     1141 gcagtttgta tattcttgtt tgcatttagt tctatagttg gtaattacta ttatggtgaa
     1201 tcaaatatgg agtttatgag tggaagtaag actaaactta atgtatttag agtttttgtt
     1261 gtaggtatgg ttttatttgg ttcacttact caagtagatg tagtttggaa tcttgcagac
     1321 ttatttatgg cattgatggc gataataaac ttaatagcaa tagcactttt aggaaaatat
     1381 gcatttattg ctttaaaaga ttatacaagt caaaaaaaat caggaataaa agaccctgtg
     1441 tttattgcag atgaaataga aggcctagaa aaggtatcag agtggcatag ggaattatct
     1501 gaggaacatt taggataaaa aatagtataa aattataaaa ccattttgaa agactagagt
     1561 atgtatatac tttatatgaa tataatattg aattaattta tattactgga atttttaatt
     1621 ccagtaatat ttttttatta tataatttag ctttattaaa agtttttatt attaaaaata
     1681 gcttatttat cgtaaagtga atgatattgg gtttttaatt attatgaatg ttagtattta
     1741 atttaaagac ttaaataaat ttgccatttc tagcgcattc atagcacaat cataaccttt
     1801 atttccagct tttgtacctg ctctttcaat agcttgttca atattttctg tagttacgat
     1861 tccaaatata acaggtaatt cactgtctaa agatactttg gctatccctt tagagacttc
     1921 actacataca taatcataat gagaagtagc ccctcttata acacagccta aacagattat
     1981 agcatcatac ttaccacttt tagccatttt ttgagcaaca agaggaatct caaaagctcc
     2041 aggtacccaa actatttcta tattttctgg agaaacgtca tgtcttaaaa gacagtcttc
     2101 agctcctgat aaaagttttg atgttataaa ttcattaaaa cgagaattta ttatacctat
     2161 ttttaaatct tttccaataa gttttccttc atatatcatt tttatttcct ccaattttat
     2221 ttatgttata gatttatatt tataaatatc tttaatttaa ttataaaaaa ctacttagta
     2281 gaaaaatatc atcatataat catttatata atatctaaaa gatgtcccat tttatctttt
     2341 ttagttttta aatatagctt attaaagtca ttaatttctt cattaatagg gattctattt
     2401 tcgattttta ttccatattt ctctaattga tttattttat ctgggttatt acttaaaaga
     2461 tttatacttt ttatgtttaa attttttaat atttgagcag ccatatagaa atctctcata
     2521 tcttcttcaa atccaagcat taaatttgct tcaacagtat cgtaaccttc ttcttgaagt
     2581 ttataagcct taattttatt taaaagacca atacctctac cttcttgctt catataaatt
     2641 aaaactccac ttccattttc aacaattttc ttcatagaag attctagctg taatccacaa
     2701 tcacatttta gagagtggaa cacatctcct gttaaacatt cagaatgaag tctaactaga
     2761 gtattttcta tatttatatt tccataagta agggcaatgt gttcttcatt tgaataaata
     2821 tctctatatc ctatgatttc aaaatttcca tattttgtcg gaaggaaagc actagatact
     2881 ttttctatag tagtttcatt tattttgcgg tattctataa ggtcttttat agtgatgatt
     2941 tttaaattat gcttttcttt gaaaatcatt aaatcatcaa ctcttgccat tgtaccatca
     3001 tctttcatta tttcacagat aagaccaatc tctttaaaac ctgcaagtct agcaaggtct
     3061 actgaggctt ctgtatgtcc atccctgaca agaactccac cagtttttgc tattaaagga
     3121 aagatgtgac caggcgtttt gaaatcttta cttgtagaat tttcatcaca gaataatttt
     3181 acagtttcag ctctttcata tgcagatata ccagttagag tatctgctcc atctatagta
     3241 acagtaaagg ctgtttgaaa tgtatctgta ttttctctaa ccataagagg tatattaaga
     3301 cttttaaatt ttttttcagt ggcaggtaga caaattagac ctctaccata tgtagccata
     3361 aaatttattg attcaggagt agccatttca gctgccatca ataaatctcc ttcattctct
     3421 ctactttcat catcaactac gattaccatt ttcccatttt ttatatcttc aatagcttct
     3481 tcaatagtat taaacatgta attcactcct ttattatttt taaaaaccat ttttaaataa
     3541 aaaatcttta tctatatttg atttatggtt actactatta gaatttaatt ccttgtagtt
     3601 attagccata aaattattaa catactttcc aattacatcg ctttctaaat taacgaaatc
     3661 tccaacattc tttgtcaaga gaatagtgtt aattttagta tgaggtataa tagaaacctt
     3721 aaatatttca tcatctaaat atgatatagt taaacttaca ccatcaatag caacagaacc
     3781 tttgagtatc attgaggata aaagtttttt atcaggtcgt atacttacta aaactgcatt
     3841 ttcatctttt tcaaatttgc agatagttcc tttaccatca acatgacctg ttactatatg
     3901 accaccaaat cttgaagaaa gactcatagc tctttcaaga tttaccttgt catttgcatt
     3961 taataaacct aaatttgtgg aacgaattgt ctccatcata acatcggctg taaattcatt
     4021 ttttccgagg tttgaaacag ttagacaaac tccatttacg gctatactat ctccaagatt
     4081 tgtgccatct aatattttat ttgatagtat ggttactttt ccactcttac cattagcagt
     4141 aatttttcta agtattccaa cttcttctac tatgccagta aacatcctaa cctcctatat
     4201 gaatgtatta acaaaaattt aatttaaaac atctgattct ataagaatat cgccatctat
     4261 caaagttaca ttattgattg ttaactgtgt tgaatctgac aaatgattta ttccttctcc
     4321 agaaacgaat gtaggtgcat ctttaccacc tataatcttg ggtgatagaa ataatttgac
     4381 tttatcaact aacttatttc taaagagact gtcattgaga gtagcaccac cttcaactag
     4441 aacagaatca atgtttaact ctcctaattt atacattaca aactccaaat ctactaagtt
     4501 gttttttgaa ggagaaatta ttgtttcaac attattttct tttaacatag ataattttat
     4561 actttctttg caacatgtga aaactatagt tttactggtt ttatctttaa ccaaattact
     4621 attaagtggc aactttaggt gtgtatctat tactattctt gtaggatgag acactttttc
     4681 ttgattgagc ctacaagtta gtaaaggatt atcgttcaaa actgtattta tgccaaccat
     4741 tatactttgg tatttatttc tgtatttatg agttagatac cttgatgatt cattagatat
     4801 ccattttgat tctagtgatt ttgtggcaat tttaccatct agacttactg ctgattttac
     4861 aatacaaaga ggtcttttgt tttttatatg ataaaaaaat gcctcattta aatctcttgc
     4921 ttcatcttct aatattccca cagtaacatc aatattgtta tcttttaatt tttttactcc
     4981 attaccacaa acaagtggat ttgggtctaa tgtagatatt acaactctct taatcttatt
     5041 ttgtataatt ttatctacac aaggaggcgt tttcccataa tgagaacatg gttctaagtt
     5101 tacataaaga gtagagtctt ttagactttg tttagcactg tttatagcat ttacttcagc
     5161 atgattagag ccaaatttct catgatatcc tttgcctatt atattactat ctttaacaat
     5221 tacacatcca actaatggat ttggatttac aaagccttct ccatttttag ccaattcaat
     5281 agcttttttc atataataaa tatctttttc tttttgattc acaaaattca caatatgtac
     5341 acctcctgtt taattggata atttttaaac aaaaaaccct gagaattaac tcagggttta
     5401 gaataatcta caataaatgt ttaataaatg tagaacaaaa ttgctctaca aaaacactca
     5461 ttaaatatct tcttccatcc agactttact gtcggctttg gaatttaacc aaatcaacca
     5521 agttttacat tggctcgcgg gctataccgc cgattgggaa tttcacccta ccccgaagat
     5581 taagtattaa atttatattt agaatgtatc ataaattatt ttactttgca aactattttt
     5641 ttaaaaaata tatgttaata tataatatag aatgtctata aactttgaaa taccacgatt
     5701 gtgaatataa aaataaaaat ttgaacataa tcaggaaaga agtctaatta atttaaatta
     5761 ggagagtaat atatgaataa aaaactttta gaaatactat caaaatacta tggatataca
     5821 agttttagaa aaggtcagga atctataata aattctatct taagcgaaaa agatgtgcta
     5881 gctattatgc caacaggagg gggaaaatca atatgttatc aaattccagc tttgatttta
     5941 gatggaatga caatagtaat ttctccactt atatcactta tgaaagacca agtagatgcc
     6001 ttaaagacta tgggaattag tgctgctttt ataaatagtt cattaagcag caaagaattt
     6061 aatgaaatat taaataatat tagaaaaaat aattataaaa tactctatat tgctccagag
     6121 agattagatg cacaagagtt tattgagcta ataaataata acaatgtaag tcaagttgcg
     6181 atagatgaag cacattgtgt atctcaatgg gggcatgatt ttaggttgag ttatagaagg
     6241 atttctgatt ttataaaaaa tttgcctaaa agacctatag ttacagcatt tactgcaaca
     6301 gcttcagaag aggtaagaac tgatataata aatttactgt gtttagaaaa tccagattat
     6361 tatataactg gatttgatag agagaattta tcaataaata tagtaaaatc atctagtaaa
     6421 aataagtata ttttagatta tattcaaaat cataaaaatg agtctggaat tatatatgtt
     6481 gcaactagaa aggaagttga aaatatatat aatggtttat ctaagagaaa tatatctgta
     6541 tcaaaatatc atgcaggttt atctaaaaat gaaagaagta aaaatcaaga agattttata
     6601 aatgataatg tggatataat ggtagcaaca aatgcatttg gaatgggtat tgataaacct
     6661 aatataagat gggttataca ttacaatatg cctcaaagta tagaaaatta ctatcaagaa
     6721 ataggtagag ctggtagaga tggagaaaaa agtgagtgtg tacttttatt ttctccagga
     6781 gatgtacata tacaaaaata tttaattgat attggagttg aaaatcctga aagaaaatta
     6841 tttcagcata agaagcttca atacatgatt gacttagttt acagtaattc atgctataga
     6901 aagacaatat tgagttattt tggagaaaat tatatagaca attgcaataa ttgtagcaat
     6961 tgtttagtag agggtgaaat agtagataaa actatagatg cacaaaaagt catttcatgt
     7021 gtagctagaa tgaaaagaag ttatggtgtt actactattg tagatgtgct tagaggttct
     7081 aagaataaaa agattttaca gcttggatta aatactttgt ctacatataa cattatgaga
     7141 gattattcta gtgaagactt aaagaatttt ataaatacat tggtttctca tggtttttta
     7201 gatttagttg agactctagg caatggaaat agaggtagtt ttcctactat aagattaaat
     7261 gagatgtcta tgaaagtttt aaaaggtgaa gtgaaagtag agtttaaaga aattgttatg
     7321 acaaaatcat tggaagtaga ggatgagtta tataatgctt taagagaact tagacattca
     7381 attgcaagtg aagaaaggat agctccatat atggtatttg gagatggtac acttagagcc
     7441 atgtctagta gttatccaat taataaagaa gaaatgctag atatctctgg agtaggagaa
     7501 attaaatatc aaaaatatgg tagagatttt gaagcagtaa ttaaagaata tgtcgaaaaa
     7561 aataatataa ataaaaaaat agatgaaaaa aatgtcgatt caaagaacgt taatagtgat
     7621 ttcttagagg ttaatacaga taaagtttta tatgaaaaac tgttagtcat tagagaagaa
     7681 tatgcaacaa aggaaaaatt acctgcatat atgattttag ccaataaggc tttgaaagaa
     7741 ataagtggaa gatatccact tgatgaagaa caattaaaag atatttctgg cataggtgga
     7801 gtaaaatttg aaaaatatgg agctagtatt ttggcagagg ttaataaata tgtaaaagaa
     7861 aataatatta atgttatttg ggagaaaaag ggcaaaagga agttaatatt agatggagaa
     7921 agtagaaaaa ataatgaaat agcattagat ttgctaaatc aaggaaaaga tatacaaagt
     7981 atcagtcaag aaatagaagt ttcactgtca accattattg gatatgtgca tgactatgta
     8041 aaggatggac atagtatatc atttgaatta ggactagaaa atttttataa tgaagataaa
     8101 aagaagatga ttttagaagt ttgtgaaaaa gttggctatg ataatttaaa taacataaaa
     8161 agaaaacttc cagattcaat aaagtatgaa aatataagag ctgtgatact tgactcttat
     8221 cttgaaaact taaaaaataa tattatagca gagtaatttt taacttaata aaaaataaaa
     8281 tttgtattat tgtgttgaac taataatttt gctgtgttat actgaatttg taaaaataat
     8341 gaaaaattag agctaggggc gctagaattg gctagctgag agataaagcc gtaacttaca
     8401 aaccctaata acctgatcaa gataatgctt gcgaagggaa gctttttaat ataattttat
     8461 tatataaata ggcaaattat tcaatgttag gagaaatttg tctaagtgta tatacgatta
     8521 taagagtgct ttccaaacga ggaaaagcac tttttttatt ttcaaaaact ataactttaa
     8581 tcttggagga ttgtaatatg aactatacaa cacaaatgga tgctgctaga aaaggcataa
     8641 taactaaaga aatggaaatc gtttctcaaa aagaacaggt agatgtaaat gaattaagag
     8701 aattaatagc aaatggacaa gttgttatac cagctaataa aaatcataaa tctttaagtg
     8761 cagaaggtgt aggtaaaaat ttaagaacaa aaataaatgt aaatctagga atttcaagag
     8821 attgtaagga tatagaaaaa gaacttgaaa aggttagagt agcaatagac atgaaagctg
     8881 aagctataat ggatttaagt aattatggta aaactagaga atttagagaa aaggttgttg
     8941 aaatgtcacc tgcaatgata ggttcagtac ctatgtatga tgcagtagga tatttggaaa
     9001 aagaattaaa agatataaca gaagaagagt ttttaaatgt aataagacaa catgctattg
     9061 atggagtaga ctttattact atacatgcag gtcttacaag aagtgtttgc caaaaaataa
     9121 aaaatcatga gagattgact catatagtat ctcgtggagg ttctttattg tttgcatgga
     9181 tggaattaaa taataaggaa aatcctattt atactaattt tgataaaata ttggatatat
     9241 gtgaagaata tgatgttaca ttgagtttag gagatgcttg tagaccaggt tgtataaaag
     9301 attctactga tgttgttcaa attcaagaac ttgtagtatt aggtgaactt actaaaagag
     9361 catgggaaag aaatgtacaa gtaatgatag aaggtcctgg acacatggct attgatgaaa
     9421 tagaagcaaa tgtagtatta gaaaagagat tatgccatgg agcacctttt tatgttcttg
     9481 gaccattggt tacagatata gcaccaggtt atgaccatat aacaagtgca ataggaggag
     9541 ctttagcatg tgctaaagga gtagattttt tatgttatgt aactccagca gaacatttaa
     9601 gattgcctaa tttagatgat atgaaagagg gtataatagc tgcaaagata gctgctcatg
     9661 caggagatat agctaaaaat gttaaaggtg ctcgtgaatg ggataataag atgagtaaag
     9721 ctagagctga tttagactgg tgtgagatgt ttagacttgc aatagaccct gaaaaagcta
     9781 agagatacag agatgaatca actcctactc atgaggatag ttgtactatg tgtggaaaaa
     9841 tgtgctcaat gagaacagtt aagaaaatat taaacaatga ggaacttaat ctaatataat
     9901 ttttggctag tatgactgga ataaaaataa gctgaaaaga ggaggtgatt tagtgaaggt
     9961 taatgggaag gaaattgaat ttgaaaaaga tttgactgtt atagatttat taaatgaata
    10021 taatttaaaa agtgatagag ttgtagttga agtgaattta gagataattg aagaatctaa
    10081 ctacaataca tatgtattaa aagacgagga cattgtagag cttattagct ttattggagg
    10141 tggttaaaat aaaaatattt gtaaatgaat tattaacaga tgttgaggaa aatacaactg
    10201 cttataaagt tagagatagc tataacaata aatgtgatgt attggtgtta aatggatatc
    10261 caattaaaac agatatgccc ctttgtgaaa atgataaatt aacacttata caaaaaggag
    10321 ttaagccatc acttgatgaa ttagaaagct tgttgattgc tagacatact ccaaatatac
    10381 ataataaatt aaaaaaaggg aaagttgcta tattgggatt ggggggactt ggttccaata
    10441 tagctatctc tttagctaga ataggagtgg gagaactttt acttatagat tatgatgttg
    10501 tagaaccatc taatttaaat agacagcagt attttataga tgatatagga aaactaaaaa
    10561 ctgatgccat gatagaaaat ataaaaaaaa taaatccatt tataaaatta aataaacgag
    10621 atattttttt aaacaagaac aatatggata ttattaagga ttcagattta ataattgagg
    10681 cctttgatga tgcttcatgt aaagcacagg tatgtaatta tgtattaata aatttaaaag
    10741 ataaatattt aatagcatca tctggtatgg caggttacta tgattcaaat ataataacta
    10801 ctaagaagat aaaagacagg ttttatatat gtggggattt tgtgaatgaa gctaaatttg
    10861 gtgaagggct tatggctcca agggttgcca tctgcgcaaa tcatatggca aatttggcaa
    10921 cgcaaatact tatagatatg taaatattaa aattagatat aaaaaagatt gaatattaaa
    10981 aaaatttaac gtggagggaa ataatatgga taaattgata cttggtggac atgaatttaa
    11041 tagcagatta ttggttggaa caggtaaata tggttcaaat aatatattgc ctgaggtaat
    11101 aaaagaaagt ggaagtgaaa taataacaat ggcacttaga agagtggatt tagacaataa
    11161 acaagaaaac atattaacgt atattccaaa agagatgaca atacttccaa atacatcagg
    11221 agctactaat gcagaagaag ctgtaagaat agctagaata tctagaaaaa tgggatgtgg
    11281 agattttata aaaatagaag taatatcaga tactagatat ttacttcctg ataatgaaga
    11341 aactataaaa gctacaaaga tacttgctga tgaggggttt atagttcttc catatatgac
    11401 accagacctt tatgctggaa gaagacttat agaagctaat gcagcggcag ttatgccact
    11461 tggagctcca attggttcaa atagaggtct tcaaatgaaa gaaatgataa gaataatgat
    11521 agatgagttg gatataccaa taatagtaga tgcaggtatt ggaaaaccat ctcaagctat
    11581 ggaggctatg gaaatgggag ctgatgcagt acttgtcaac actgctatag cttcagcagg
    11641 agaccctgta caaatggcta gagcatttaa attagctgta gaaggtggaa gagaagcata
    11701 tattgcaaaa acaggaaatg tgtcagaatt tgcaaatgca tcttctcctt taacaggttt
    11761 tttaggcaat ttatagaaag tggtgattgg tgatgagttt ttatgatgta atagaaaaat
    11821 atagggattt tgattttgat ggatatttaa ataatgttac tgataatgat gttttaagaa
    11881 gtttatcaaa agacaagctt gaagattttg atatattaaa tttgctttct aaaactgcag
    11941 taaaacattt agaagatatg gcacaaaaag cacataaatt aagtgttcaa tactttggta
    12001 aaacagtatg tttatataca ccaatgtata tagcaaatta ttgtgttaat caatgtgttt
    12061 attgtagcta taatataaag agtggaataa aaagaaaaaa attaactatg gatgaaatta
    12121 gagaagaggg agaagcaatt tcaaaagaag gtttcaaaca tctacttgtt ttgactggag
    12181 aaagtagttt tcactcaagt gtagaatata taggtgaggc tatagaaata ttaagagaga
    12241 aatttccctc tataggtata gaagtttatc caatggaagt agaagaatat aagtacattg
    12301 tagataaagg tgttgaagga ttaacagtat atcaagaaac ttatgacgag gaaatatata
    12361 agagagtaca tataaaaggc ccaaaatcaa attataaatt tagactagat gctcctgaaa
    12421 gaggggcaaa agcaggtatg aggactttat caataggtgc acttttaggg ctaaatgatt
    12481 ttagaaaaga aacttttttt acaatattgc atgggaaata cttaaaaacg aaatatcctc
    12541 atattgagct ttcatattca acaccaagaa tgagaccttt taaagggtgt tttgaggaat
    12601 tggtagatat aagtgatact gatttagttc aagctatggt atgtatgaga ttatttgacc
    12661 ctcatgcagc tataaacata tccactagag aaaatttaga aatgagaagt catataatac
    12721 ctttaggagt aactaaattg agtgcaggtg tatctactga tgtaggaggt cattctcaag
    12781 atgaacatga tactgctcaa tttaagataa atgatgaaag tactgttaaa gatgtggaga
    12841 aaatgctaaa ctcaattggt tatcagcatg tgtttaaaga ttgggaaaga ttttaaatgt
    12901 atctgataac aaacagaaaa ttatgtagtg aggaacgata tttagaagtt attaaagagt
    12961 ctatattgag tggtgtagaa aatataataa taagagaaaa ggatttagaa tatcaagagc
    13021 taagaaaatt atatatgaaa ataaaaacta aaatcaattg tatagatttt caagagcaaa
    13081 tatcagatga aagtttgaaa actaatataa atcaaaaaga gtttagaaat aaattcaagg
    13141 ttaattttat aataaatagt aacattgaat tttttgaaaa agtggattgt caaggtatcc
    13201 atcttccttt taaattattc ttgaatttaa ttgaaaataa gtataatttt aatgaaaata
    13261 aaatactagg attatcttta cataaagtag aggaagtaga ttatttagaa aaattagtaa
    13321 gaaatcaaaa tataaaaata gattacataa cattatcaca tatatatgaa actaaatgta
    13381 aagaaggatt aaatccaaaa ggaattgagc ttttaaaaga agctaaaaag attactgata
    13441 ttaaaataat tgcacttggg ggaattttac catctaatgt caaggaaaca ttaaaatact
    13501 gtgatgattt tgctataatg tcaactataa tgaggtctaa agatattaaa aaaaccatat
    13561 caaattataa tgaaaagttg aattagcaaa tatagtatat tgaattttta acttaaataa
    13621 aaataaaaaa gttatctttt aaattttaaa aggtagcttt tttattttat atctttgcta
    13681 ataaaaaatt gtaaaaaggg aaaactttag tcagagagat taaacaatat cctagaaaga
    13741 tttaaaattt agtaaagtat ggaggacatt ataatgactg acagtaaaaa gaaacgattt
    13801 attataccac acacttttac aataatattt ttactaatag tcttgatggc aatattgact
    13861 tggatagtac caagtggtga atttagtaga gaagatgtag aaggtagaat ggtagttgta
    13921 ccagggactt atcaaagtgt agaaagtaat cctcaaggat ttgcagatgt ttttaaagca
    13981 ccaatagaag gctttatcga ttcagctgaa gttgtaggtt ttgttttaat agttggaggt
    14041 gcttttggta ttgtaaatag gactggagct atagaagcag gaatagcttc ggttatctct
    14101 aagtttaagg gaaaagaact tcttataatt ccaatatcaa tgattttatt tggacttgga
    14161 ggtacaacat ttgggatggc agaagagaca ttaccatttt atatgatatt tgttccactc
    14221 atgacatcct tgggatatga ttctctaaca gcaatagcaa ttgtatttat aggagcagca
    14281 gcaggagcag ccgcttcaac aataaatcca ttttcagttg gtattgcaca ggcactagct
    14341 caacttgctc ctggttcagg agtaacttac agagtagtta tttttatagc atatatacta
    14401 ataagtatag catttgttat gtactatgct aagaaggtaa aggaaaatcc tgaaagttca
    14461 atagtatatg aaatagataa aaataataaa tcaatactta atcaaacttc aacagaaata
    14521 aaagaattta cttttaaaga agtatctgtt cttgctatat ttggaatagg gatggcaata
    14581 atgatttggg gtgttttaag tcaaggatgg tacattccag aaatatcaat gtgttttgct
    14641 gcaataggtg tgctttctgg attggttggt agattatctc aaattgaaat ctcagaaagt
    14701 tttatagatg gagcaaaaga tttagcatct gcagctcttg ctatagcttt ggctcgtgga
    14761 atcgtaataa ttgctcaaaa tggatttata atagatacta tattaaattc cgcagcagcc
    14821 tttctaggtg ggcttccaaa ggttatattt gtatatttat catttttctt ggaagctgca
    14881 ttggcatttt taattccatc atcttcaggt cttgcttcac ttacagtacc tgtacttgct
    14941 cctcttggtg atttggttaa tatatcggct caagtgatag taaccgctta tcaatttggt
    15001 gtgggtctta caaactttat aactccaact tcaggagtac tgatgggagc acttgcagta
    15061 gcccatattc cattttcaaa atgggtaaga tttataatgc ctatcttgtt gattttgaca
    15121 gctgtatctc tagtcttttt aacaataggt atttatatag ggttttaatt aagtgtagtt
    15181 ttatttaaga tatttagtaa tggtataaaa tgataatatg ttttcaataa attgccacta
    15241 atttaattag tggtaatttg ttttttgtat ttaaaaataa atgtatttac ttgtattttg
    15301 aattaatatc tacttagaca ttgttttaat tgttatattt ttatttaatt aagcattgat
    15361 ttatcatttt ttatatttca tataacaatg caaaaaataa tttttatata gtaaatgaca
    15421 aaagtttaca ataaatgtat attttcttac tcattcctta aaattactca tttagtttaa
    15481 taactctcat ttcatatgta aagttggtaa aaaaattaaa attaaaagta aaaaactaaa
    15541 taaattaatg ttaatttgaa atatatatga aggtttttgg agaatactta cttatgatag
    15601 gaaaaaatgt taaaataata gtgtaatcaa atataaagga ctgaaataat taacagttat
    15661 taaatattag gagtgtaaaa atttatggga ttaaaagaat ttgattttat tgaagagtct
    15721 attgatatgt tgaggacaat gtcaccaaca ttagaaacta tatcagatga aatagaagaa
    15781 tattttgaaa atatcttgga tgaaaagaat caagaatata taaatgtaac ctctagaata
    15841 aaatcagaat caagtttaag agaaaagata attagaaata gatatttaaa aaaatatgga
    15901 gaagcaagta atcttattca taatgtttct gacttaatag gcctaagaat tgagtgtaga
    15961 tttattgagg atgaaaacaa aatatataga cttttgagaa gatattttaa taaaacagat
    16021 gacaaaataa actattataa taaagagaat aagaatatta aattaaaact atctgagagg
    16081 caacccaata agcaaaaaaa tggatttgaa atatataaaa tagatggagt ttttgcgtat
    16141 ttagatagag aagtaaaatt tgaacttcaa attaaatctc ttgtaaatgt gttttggagt
    16201 gaaatagaac ataaaataat ttataaaaac aatacatatt tacttgctga taaatttata
    16261 aaggatatga tggactctat aaagaataat cttactatga tagataatca gcttttaagt
    16321 atttataaaa actttcattc agggaagtct tttaatatga aagtaagtaa aaaagaaatt
    16381 gaaaaattat ttgcgaaatt agtctatgac gctttttctg aaaagatgaa taaaagtata
    16441 ggttttgttg tagattttaa aaaaccatgt gagactatat tgaactattc ttttaataag
    16501 caagatataa gtgatgaagt tttaggtaat tttatgcttg atgagtttgc aagattaaat
    16561 gaaattgtaa acaaggatat agattttaat gagcaaatag aatttgaaag agaaccaaca
    16621 tttgatgata agttttgtaa agacctagga aatcatttta gaagtagatt aaacacagaa
    16681 tttccatgga acctattttt tagaatatta tttgaaatag agccatataa caataccaaa
    16741 gactttgaaa actttgtaga gtttataaaa gaaaatgtgc tactagaaga aaatagggag
    16801 caattattat gccaatttga agaagatagc aaacttataa ttgaagatat atatgattgt
    16861 atatttaata gtataagtga gattgatagt gttgaaattt tatataacta taatcttgaa
    16921 aaaataaact ttacatcaag tgaaatcata aattgtatat gtagagaata tgagtgttat
    16981 ggtgaatact tagaagaaaa agaaaagctt atgaatacat ttaaagaaaa gattattcaa
    17041 atctttgagt aataggttaa tgctgaagtg gcgctaatgc gccatttttc aaatatatat
    17101 gaattaagta aaattatttt ggaggaggcg atttgtatag agaaaacagg tgtaattttg
    17161 gcagggggaa gaaattctag aatgggtaga gataaagcct ttttagagtt acatgataag
    17221 ctttttattg aaattgcaat agaagcattt aaaaactttg atgagttgat aataatatct
    17281 aataatgaag aattatattc taaatacgat attaaagttt ataatgatat agtaaaagat
    17341 gttggtccaa ttggaggaat atatactgct cttgaatatg caaagtatga tatagttaca
    17401 attgcatgtg atatgccata tctaaataat caaatagtcg aaagaatagc taataaaatg
    17461 gatgataaaa gtgtgatttc agtgacaaat ggaaagttac aaccattgtg ttctggatat
    17521 aaaaagagta taataaataa agtatcttta tgtataaggg aaaatgattt aaagctcaga
    17581 agctttatag ataaaataga taaaagttat atatactttg atgaagaaga tttattttta
    17641 aatgtaaaca ctgttgatga gtatgaaaaa ttaactaaag tttaattagc tatggttgta
    17701 aaatagagta ggatttatat ggaggagagt ttatgaattt acttaaatgc gattcttggg
    17761 cagtaatatt aaataatagc gataaagaaa gtaaagcata taaaatatta gatgaactta
    17821 aaaggaatat gtacaaagtt gttgctatag atgaagagaa aaaacctatt gaaggaatag
    17881 atgtgtatga atgtttaaaa gatgttccac acaatattga tgttgtagct attattgata
    17941 aacaatctaa gatggatgtg attttagaag aagtagagct tttggatata caaaatatgt
    18001 ggtttgaaaa aggaagcttt agtgaaatga tgattaaaaa gactaaagat ttaaaattaa
    18061 atatagaata taatttaagt ttatatgatg agttgactag ataaaattat atatcttaat
    18121 aataaaaata taattagtta ataaaacata ttattttata gtatagattg gttaataaaa
    18181 gtacagattg gttaatgaaa cattaaatta caatatttgt taaaaaaatt gtgagttaca
    18241 tgatataatg tattaaatag gatactaagg agttgatata ttatgttaat agtaactaca
    18301 gaaaaagtag aaggtaaaaa gatatcaaag gttttaggat tagtgagagg aagtacaata
    18361 agagcaaaac atgttggaaa agatatagga gcaagtttta agaatcttgt tggaggagaa
    18421 cttactggat ataatgaaat gctcactgaa gcaagacaaa ttgccatagg tagaatggtt
    18481 gaagatgcag aagctaaagg tgcaaacgca gtaatagcat ttagactgtc ttcagcttca
    18541 gttatgcaag gggcagcaga aatgcttgct tatggaacag cagttgtttt agaagatgat
    18601 aatagtattc ttgaaaaata aaatataaat agttaaaaag aggcatcttt taaaaatttt
    18661 atggatgcct ttttttattt gaaaaaacta atttgagaag aaattttaac tattatgaca
    18721 ataaaattag ggtgatatta tataaataag taaaattata ttatataatg tatggtatag
    18781 actttaaaaa ttttatttgt agagaaaaat atggacaatg ttataataat tatagaaatt
    18841 aaaagtactt acattcatta tgtatgtgac atgtataatt agttttatat ttaatacagt
    18901 tttgaagtaa tatcttcatt ttga
//
LOCUS       DAEMIK010000079        18820 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEMIK010000079 DAEMIK010000000
VERSION     DAEMIK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.079"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            302..1519
                     /locus_tag="KPK87_002258"
     CDS             302..1519
                     /locus_tag="KPK87_002258"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG2538405.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1614..1994
                     /locus_tag="KPK87_002259"
     CDS             1614..1994
                     /locus_tag="KPK87_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2538406.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2278..2760
                     /locus_tag="KPK87_002260"
     CDS             2278..2760
                     /locus_tag="KPK87_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2538407.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSDVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2781..3596
                     /locus_tag="KPK87_002261"
     CDS             2781..3596
                     /locus_tag="KPK87_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2538408.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3649..5199
                     /locus_tag="KPK87_002262"
     CDS             3649..5199
                     /locus_tag="KPK87_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2538409.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEDY"
     gene            5296..6648
                     /locus_tag="KPK87_002263"
     CDS             5296..6648
                     /locus_tag="KPK87_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2538410.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIVRGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6779..7537
                     /locus_tag="KPK87_002264"
     CDS             6779..7537
                     /locus_tag="KPK87_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538411.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITFKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7584..8303
                     /locus_tag="KPK87_002265"
     CDS             7584..8303
                     /locus_tag="KPK87_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2538412.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHDGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8427..9827)
                     /locus_tag="KPK87_002266"
     CDS             complement(8427..9827)
                     /locus_tag="KPK87_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG2538413.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9831..10739)
                     /locus_tag="KPK87_002267"
     CDS             complement(9831..10739)
                     /locus_tag="KPK87_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2538414.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            11000..11647
                     /locus_tag="KPK87_002268"
     CDS             11000..11647
                     /locus_tag="KPK87_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2538415.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11972..14611
                     /locus_tag="KPK87_002269"
     CDS             11972..14611
                     /locus_tag="KPK87_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG2538416.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14669..15772)
                     /locus_tag="KPK87_002270"
     CDS             complement(14669..15772)
                     /locus_tag="KPK87_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG2538417.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16132..16953
                     /locus_tag="KPK87_002271"
     CDS             16132..16953
                     /locus_tag="KPK87_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2538418.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    17032..17243
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17330..18646
                     /gene="argH"
                     /locus_tag="KPK87_002272"
     CDS             17330..18646
                     /gene="argH"
                     /locus_tag="KPK87_002272"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG2538419.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7213 a         2153 c         3321 g         6133 t
ORIGIN      
        1 taaaatcaaa aaaatagatt ttgggtaaat aaaataatat aaaatataaa ttttaataaa
       61 aataataaag tatatattat tgctttaata agaatgtaaa aaatattttt aaattatcat
      121 ttaatgtaat ttaaatgaat tgaaaatata tgtttattgt atgcaattgt tgtattttta
      181 ggttttttgt gattaattaa aaaattataa aaatcgttta catgttgatt tttaaaaaaa
      241 aaagatatag aattatatag aaagtaataa acaaaaattg gatttacggg agtggaatga
      301 aatgatgaat gttaccttaa cagatgtgaa agaggctaga gagaccatta agaacatagt
      361 aaaaagaact gacttacttg aaagtgttaa acttagcgaa aaaacaggag caaatgtgtt
      421 ctataaatgt gagaatcttc aaaaaacagg ttcttttaaa ttaagaggag cttgtaacaa
      481 aatagcgagt ttaacagatg aagaaaaggc aaatggagtt atagcttcaa gtgcagggaa
      541 tcatgctcaa ggagttgcat tgggagcaaa aatgactggt ataaaatcta caatagtaat
      601 gccagcaact gcgcctttag caaaagttag tgcaactaaa ggttatggag cagaagtagt
      661 tttaaatggt gcggtttatg atgatgcata tgcaaaagct gttgaaatac aaaaagaaac
      721 aggagcaact ttcttacatc cattcaatga taagtatgtt atagcaggac aaggaactat
      781 atctcttgaa attttcgaac aattagacaa taaagttgat actatactat gcccagttgg
      841 tggaggagga atcatctctg gtgttgctgt tgcagcaaaa gcattaaatc ctaatgtaaa
      901 aatagtcggt gttcaaactg caaatatacc ttcaatgaaa gagtcaataa aaaatggaaa
      961 agtaacaaca gcatttaatg atactacaat agctgatggt atagctgtta aaactccagg
     1021 agatttaact tttgaaataa taaatgaatt ggttgatgaa atagtagtgg ttgaagagac
     1081 agaaattgca gaatcaatat tatttatgat ggaaagtcaa aaaatagttt cagaaggtgc
     1141 tggtgctgta tgtacagctg ctatattaag tggtaagtat gtaccagcaa aagatgaaaa
     1201 tgtagtttgt ataatatctg gtggtaatat agacataaat acgttatata gaataatagg
     1261 tgttgcttta gctaaagaag gaagaagata ttcatttagt acaattatgg aagataagcc
     1321 aggaaacttt gctgagttaa ctagaataat aagtgagaat ggtggaaaca ttttaagtgc
     1381 taatcaaggt aaattatcag caggggaagc tctaggaaaa caaagtgcgg aatttatatt
     1441 agagacaata gattatgacc atatagctag aataaagaaa gctatagaag aaaaaggttt
     1501 taaaattata gaattataat aaaaagtgta tataatatac aagaaaaata gtataatagt
     1561 gtatgaagat acattttatg tgtattagat taacgaatta ggaggatttt aaaatgaaac
     1621 atgaagtaat acatactaat gatgcaccag cagctttagg accatattct caagctataa
     1681 aagctggaaa cttgttattt gtatctggac aagttcctct tgtaccagag actatggaag
     1741 tagttgaagg agatgtacaa gctcaaactg ctcaatcttt aaaaaatctt aaagctatat
     1801 tagctgaaag tggagctgat ttttcaaatg tagttaagac tacagtattt ataaaagata
     1861 tgaatgaatt tggggctata aacgaagttt atgctgaata ttttggagaa aataaaccag
     1921 caagagcttg tgtagaagtt gctagattac caaaagacgt taaagttgaa atagaagtaa
     1981 tagctgtatt ataaaaatac aatattttta atttgaaaag gctgaaaaac tatattctag
     2041 tgattcagcc tttttataaa aaaataaatc acaataaaga acatgataaa tattgtcgaa
     2101 ttaaaaaata aaattaaaat aaatattgca atatttttga gaaggtttta taatgagtat
     2161 ataatttgaa acgtgtaact attaaattag gcatgagttc ttggatattt ttatcctgag
     2221 gattcatgcc ttttttgtat tgaaattcaa aaatctattt aataaggagg aagtactgtg
     2281 ttaaattttt tcaagaaaaa taaaagctat aagttacatg cagtagtgag tggtaacagc
     2341 attaatattg agaaagtaaa cgattctgtg ttttcaaaaa agttaatggg agatggagtt
     2401 gcaattattc ctaatagtga tgttgttgta gccccttgta atggtaaagt aactgtgctt
     2461 acagaatcaa agcatgcctt tggtatggtc tcagatgagg gtgttgaaat attagtccac
     2521 ataggaattg atacagttag tttacagggt gaggggttta aaaatgaagt gtctcaagga
     2581 gatactgtta aaaaaggtag tccaataata agttttgaaa gagaaaaaat aaattctcag
     2641 ggaattgatt gtacaacaat aataattgta ttaaatcata gcgaattttc agaaataaat
     2701 tgcatggtag aaaatgaagt tgttgcaggg caagatactg ttattgaaat tatgaaataa
     2761 taggaaggga ttaattcaat atgagagtag taaaaatctt taataataat gcattatcta
     2821 cagtaactaa tgataggaaa gaagctattt tactaggtct tggtataggt tttaataaac
     2881 gtccaggaga taaagttaat gaagataaaa tagaaaagat ttattatgtt caagaccaca
     2941 tgcaaacaaa atttttagaa ttattaaaaa atgttagtcc agaagtgatg gatgcttcac
     3001 aacaaattat aagtcttata ccaggaaatg agggaaaatt taataataaa gggatattat
     3061 ctttagtgga acatattagt tttgcaatag atagaatgaa aaataatgtt tttttaccta
     3121 atttaatgct tttggatata aagatgatgt attcaaaaga gtttgaatta ggagtaaaag
     3181 ctttagaaat tatatataga gtatgtcata tccatttgcc tgaagatgaa gcaggttaca
     3241 tagctttaca ttttgtcaat ttacaatcaa atgataattt ggcatatgat actttaaaat
     3301 ttgtaaaagg tagtattgat ttgattaaag aatgttatgg attagaatta gacgaatcga
     3361 gtttaagtac attacgtttt agaactcatc ttaaatttct tgctcaaaga atttttaaga
     3421 atgaaatttg tcaggatgat aaaatgattg aaatgtatga ttatttaatt aacaatcatc
     3481 ctaaaaataa agaatattta gaaaagttaa atgtatatat tgaaaaggag tttaggtata
     3541 aattagataa acctgaaaaa atttatctac tactacacct aacaaaaatc ttataagaat
     3601 gagatgaatt agtaatagaa atatatataa ttttaggggg ggataagatt gaaagaaaaa
     3661 ctacttggag gactggaaac atttgcaaag gcaatggttc agccattaat gtatttatcg
     3721 agtgcaggaa tattaatggt ggtaggtgta ctacttacaa atgatatgtt attaggagta
     3781 cttccatttt ttaaatggga gccaattcaa atatttggaa gcctaattta taactgtata
     3841 atggctatta ttaataatct tgctgttata tttgctatag gtattcctgc tgcactagca
     3901 aaaaaagaga agcacaaagc agcgttaata ggatttatga gttatataat ataccttaca
     3961 gcttccaaca ctatgttaac atcactaggt caactagcaa aagaaagcaa gatgcttgga
     4021 ctgataggga ctggacaagc tactatttta ggtattcaag ttgtagatac aagtgtattt
     4081 ggtggaatta ttttagggtg tatagctgga tatgtattta atcgtacaag caacaaaaca
     4141 tttaaaggag ctttacagat atactcggga gcaaatttta gttttatatg catgttcttt
     4201 gtatccattt taattggagt tgtgagtaac tttatatggc cattagctca acaaggaata
     4261 gcttctcttg caaatgtcat caaagataca ggtacatttg gtttattctt atatggtttt
     4321 ttagagagaa tattagtacc aactggactt catcatttaa tttatacacc atttcagttt
     4381 tctgatttag gaggagtgct taaagttggt gcagatacag tttcaggagc ctatgctata
     4441 gttatgacag aaatgaattt accagttgag cgtttctcag atagtatata ctacatggca
     4501 actggattta caaagatgtt tggttatata ggtataggag ctgcctttat ttatactgca
     4561 tataagcaaa ataagaagaa ggttaaggca acgattattc cactaataat tacggcagta
     4621 ttagcctctg taacagaacc attagacttt atgtttgtat tctgtgcacc acttttattc
     4681 tttgtacatg cagttatagc tggtcttttt atagctttat taaaaatatt tgatgttact
     4741 gcattatgtg gagggaactt attagtatca attataatga atactacagt aggcgtagaa
     4801 aaaacacact ggccagtaat gatgatatta ggacttgcac aaattattgt atattttgta
     4861 gtatttagtt ttttaattaa gaaatttaat tataaaactc caggtcgtga agatgagagt
     4921 acagctacag gcgaagaaca atcaaaacaa cttaatttag atgcaggtat agaaaatata
     4981 attgatgggt taggcggaaa agaaaatatt aatacagtag aaaattgcat cactagatta
     5041 cgtgttaatg taaaagatga aagtaagatt aatgaagata taattaattt aacgccaaat
     5101 agcgggattg taaggaaagg aaaagatata caaataattt ttggacttca tgtgcatgag
     5161 gtaagacgtg cagtagaaga ttttttagaa gattattaaa gatatatatt tatatagcaa
     5221 gggtaaataa cagctttaag tgctgtttac tatttattaa agatacatat aatttataga
     5281 ggaggaataa ttattatgat tattactatt gttggtggag gaagcacatt tactccaggt
     5341 attgttaagt caattgcgtt aagagaaaaa gagttagagc ttgaggaaat tcgtttattt
     5401 gacattgata aagaacgtca agataaagta gctgtagttg tcaactggat actaaaagaa
     5461 gatgttaaaa cagatgttaa actaactgta actaattgtg aaaaagaagc gtatacagat
     5521 gctacattta tatttgcaca aatgcgtgtt ggtaaatatg aaatgcgtga gcaggatgaa
     5581 aaaattccat tgaaatatgg ttgtgtaggt caagaaactt gtggttgtgg agggatggca
     5641 tacggactgc gtacaataca accaatgata aaggttattg atgatgtaga gaaatatgct
     5701 aaaaaagatt attggatatt aaattactct aatcctgctg caatcgtttc tgaagcttgt
     5761 cgtaagttaa gaccaaatgc aaggattata aatatctgtg atatgccaat agctattata
     5821 gatatgatag cggggtcatt aaatatcaat gacgttcata atatacgcta tgattatttt
     5881 ggtctaaatc attttggctg gtttacttca atagattata aacatagaga cttaatggaa
     5941 gagattaagg aatacataaa agaaaataaa atattattac caaaatctta tatagaaaac
     6001 ttaaataaaa gtcgtagtac aggaaatcgc catgctgtaa gtagctggat acatgtttgg
     6061 gaatctgttt atacaatgtt agaaagcttc ccaaaatatc tgccaaacac ttatttaaca
     6121 tactatcttt taagtaaaga aacagtggaa cattcaaaca taaatcgtac tcgtgcaaat
     6181 gaggttatgg aaagccgtga aaaagactta tttgaaggtg tgagacatta cttagaaact
     6241 ggagaaattt ctgaaaaagc attttatgca ggttcacatg gagattggat tagtgatttg
     6301 gcagtttcaa ttaaaaatga tacacgtagt cgttttttag ttataactga aaatcgtgga
     6361 gctataccaa acatgccata tgatgcaatg gtagaaattc cagcatatat tggtaaagat
     6421 ggtccagaag ttattgttcg aggtccaata cctctattcc aacaaggatt aatgatgcaa
     6481 caacttaaca gtgaaaaatt gatagtagaa gcttatatag aaaatagtta tgaaaaagct
     6541 ttacaagcat tcacattaaa taaaactatt ccaagtatga atgttgctaa agaaattttg
     6601 gatgaaatga tagaagttaa taaggaatac tggccagaat taagatagta ggataaaaga
     6661 aagggttttt atttaataat tagatatgtt ttcagagtct tttatcaaaa tgtattcaca
     6721 aacatatggc aatatattaa aataaagagt gatattttta gaagaatggg gtgatgcaat
     6781 gaacattata aataaaattt tttggcaaga agataggatt ggcatgatta caaataattt
     6841 agaggcagat acacatagcc actgtatgtt acaactgttc ttagggatag aagacagcat
     6901 tgaaataaca gtcaatgaaa aattagttaa atgtaattgt atcatagtag ataaaaatat
     6961 ctctcattca ttttcagcaa gaaaaaaagt ttattactct gctattattg aacctacatc
     7021 tatatatgca gaacagctta cctcaaaaat gaatgatttt ggctactgga tatgtgacaa
     7081 tgatggatta gagaaattac gtcaacaagg aacttttttg atagataact ctagtaaaga
     7141 acaatattta agatttatgg aaatgcttaa taactatttg aatataccta taactttcaa
     7201 acattatgat gacagaatta ctgaattgct caatttatta cgtacttgta attgtgataa
     7261 tcatactatt tcaagttttg cagataaagt gtctctatca gcaagtagat tatcacatct
     7321 gtttaaagaa caaataggga tacctttaaa aagctatatc ttatttcatc aaatggagtg
     7381 tgcttttaga gaattacttt ctggaaaaaa tgttaccgaa gcttctatga ctgctggatt
     7441 cgatactcca tcacactttg cagggacagt taagcgaatg atgggaatgc ctgtttcttt
     7501 atctttgaaa gatagcgagt ttttgaaagt atattaaata ttctcgttat ataatgaagt
     7561 tgtactaaga aaggggcttt gttatgaata aagaatattt agaagaaaca aaaatgttaa
     7621 attataatga accacaactt aagcttttgg tatcatcaaa gaattggttg gaattagatg
     7681 attttcataa aataaaatct atttatgagt ttgtacagaa tgatatttta tttggatata
     7741 atgcttctga tatgttaagc gcaacacagg tattaaatga tggtatggga caatgcaata
     7801 caaaagctac tcttttaatg gcattattac gtgcagtgaa tatcccttgt aggcttcatg
     7861 cttttgatgt aacaaaagat tttcaacgag gagctacatc caaattgatt tcactattag
     7921 caccaaaata tattcttcat acttgggtag aggtatttta tcaagatagg tggattgctc
     7981 tggagggtgt cataactgat aaaaaatatt tagaagcaat tcagaagaaa ttttttaatc
     8041 atgatggaac gtttaaaaag tatgcaatag ctacaaatga tttgaaaaat acatctattg
     8101 attgggatgg taaagatact tttatccaaa aagaagctat cgtgtatgat tatggtattt
     8161 ttccatcacc agacgtattt ttttctactc attcacaaca tatgtcaaaa ttaaaaaatt
     8221 tcatatatgt acatcttatc agaaaaataa tgacaaaaaa tgtatgtaag gctagaaata
     8281 attatattga taaaaatgaa tgagtgttta agaggtaact atataggaaa tatatagaat
     8341 aattaaaact gtactggtta aaatgaataa aaaggagtta tcacagagca aacttaaatt
     8401 gttcatagga taactccttt tctgtattaa cttctaataa tttgatgtct ttttataaaa
     8461 catatagata caattaaacc agtaaatgca agagtagctg ctattgaaaa tgcaacatct
     8521 attccatgaa tgttagacag catgtttgaa ctcatatgag ctttcttagt agcacttgtc
     8581 attatagtaa taagtatagc agaacctata gctccagata tctgacggag tgtgttatta
     8641 attgctgtgg catgagatat aagttctcta tctaatgttt taattcccca tgtagttaat
     8701 ggcattaaaa ccattgaaat accaatcata cgaatacaat acatcaatga cacatgaatt
     8761 aaagatgtat ctctgcctaa aaaagcgaag gataaagttc ctaaaaacaa acatcctgtt
     8821 cctaatattg atagagcatg aggtccatat ttatctaaat gacgtcctgc aattggattg
     8881 agtacaacca tcaatatagc accaggtaac attaatgaac ctgaagataa tgcagaaaat
     8941 cctcttacag attgaacgta cagaggaatc attaatgtag cagacatcat tgaggcatat
     9001 acaatcataa ttaatattgt agaaataaga aatggtttat ttttaaaaac cctcaattct
     9061 aaaaaaggtt ctttagattt taattgacgc aatgtaaata aaattaatga cataattcct
     9121 attaatattg gtaaatatgt ggcaatatta gtccacccat aattaccttg attagtaaat
     9181 ccaattaata aaccaccaaa acctagagat gatagtacta ttgaaggtat atctaatttt
     9241 agtttttgag tttccccaac atttcttaat agaataaatg atagtataac atctaatata
     9301 gcaatagggg aaagaaacag aaataaacta tgccaaccaa atgaatctac taaccatcct
     9361 gataaagttg gacctattgc tggagcaaat cctactgtaa taccaactaa actcatagct
     9421 gcaccacgtt tttcctcagg gcataaatag agtataataa cttgtaaaag tggcattaga
     9481 gaaccagaac ccattgcctg caacacacga gcaatcaaca tagttgaaaa attattagaa
     9541 aaaagtgcaa taatacatcc tatagaaaag atagacattg atgtaataaa taactttcta
     9601 gtactaaatc gatttattaa ataagctgtt gtgggtacaa taatacctgc aataagtaga
     9661 taaattgttg ttaaccactg accaacatca gcagtaattt taaaatctga cattatatta
     9721 ggtaatgctg ttgacaatag tgtttgactg agagaactta taaaagcgcc agtcattaca
     9781 attgctatta ttaaatttcc ttttttattg ttactttggg tttccataat ttataacgtc
     9841 tcctttttta attgattttt aatgctgttc aataaaagta cttttggtct taaagtgtct
     9901 tctttttcta aatatgcaat cattgcatcc ataattaaag aatcatattg ttgatttttt
     9961 ccttcgacgt ataatttttt tattgcttct agtaatttag gattttcaag gagagaagta
    10021 tcagttttga ttagttcttc tatatcttgt atcgttttaa aaaatagcga tttattatga
    10081 agggaaaagt aaacttcatc ttcttgttta tgtaaaaaca ttgatttttc aattaaagga
    10141 tttagattat ccatattttc tacaactaaa tctagaattt gtatagtaaa tttggcaata
    10201 aagtatacat ttatttctct tttttgtaca tgaataatgt atataaactg atgaattaga
    10261 gcatccaata agaaaactaa atcaaatata attttttcta ttttatcacc atagttatca
    10321 aataagctta gtttgtattc attcaataag ttagctcttg attttgtttg caatttactt
    10381 gtagccttac gttgctcttc agaaaagttt tctcttatat aggtatttat ggtagttcta
    10441 gaaaaaatat ttttccatac agcaattatc tttctgttta acttttctga tgatggaatg
    10501 tcattatttt catttatgga atgtataatt tctaaaaact tttcatcata ataattgatt
    10561 atttcgcaga taaaactttc cttagtttca aaatgcttgt aaaaagtagc tttagacatt
    10621 ttacactctt ttacaacatc cgatattgaa gtattttgaa aacctttttg gtcaaaaatt
    10681 ttttgtgcaa tagttataat gagcctttgt ttttctgtca ctttattatc tcctttcacg
    10741 caaaaacgtt tagtactata tagtacctac aagttttgat aaatatatta tacatcattt
    10801 ggaaaaaaaa tcaatatata gactgtaata tgaaattttt gaaaaatttg ttgttatttt
    10861 tagtcgtata aaaaagaaac ttaagagttt cttaagaatt gtttaaagaa gttgcaataa
    10921 attagtggta tgattatagt atgaaagaga atgtcttgaa atttttctgt atcaaattcc
    10981 aattatggag gtgtagtata tgaaaaagaa aatttgtgta gcaataattt ttgctctatt
    11041 tatagttttt gtagaaatgt ttattgatag ctctagcgaa ggaagtaaaa ttatgacagc
    11101 tgaattaagt aaatacagga aaggcgtaaa tgtattatca ataaacaata ttatagtagc
    11161 gaataaaaaa tatagtttgc ccaaagatta ttctcctcag gaaagcagtg aagcgagaga
    11221 tgctttttat aaaatgaata aagatgctca aaagtcagga ttaaatttaa aagcttttag
    11281 tacatataga agttatgaat atcaagatag attatttaag tcatatgtaa aagaacatgg
    11341 agaaaaagaa gcaaatagat tttcagctaa acctggagag agtgagcatc aaactggact
    11401 tgcttttgat attggaggag atgaccagtc ttgttgggca aataaaaagt tcaacaatac
    11461 aaaagaagct aagtggttgt atgaaaatgc atataaatat ggttttatac ttagatatcc
    11521 agaaggtaaa gagaatataa caggatatat gtatgaatct tggcactata gatatgttgg
    11581 aactgaacat agcaaaaact ttgcaatgaa taatttgact ttagaagaat atttacatat
    11641 aaattaagag atggtttgtt aaaattaggt taaaataaaa aataacctag actattagct
    11701 gtgtaaagtg atataatgta aatacaattt aagataacta ctcgaagggg agtagcttac
    11761 acgaaagtgg taattaatcg tcaagacagt agaaatactc ggttaattag caagggattc
    11821 cttgatagca agaccttcaa aataagcatt aggtatgttt gttttggggt ctttttttat
    11881 tcaaggaatt tttatttaaa aaaatttaaa tttcaagtat acataagagc ccccaaacat
    11941 aaaataaatt tattgaagag gagggcaaca aatgtcttac aaaagtactt atcaagaagt
    12001 aattaaaaat ttagactcaa gtttatctgg tttgagtaaa gaaagagcag aagagttgtt
    12061 agaaaaaaat ggagctaatg aattaaaaga agcagataag gttcctactt ataagctttt
    12121 cctagaaagt tttaaagacc cattagtaat aattctattg atagctgctt tagtacaaat
    12181 atttttaggt gaaacagtgg agtctataat aatttttgca gttattatta taaactctgt
    12241 tctgggtgtt gtacaaacaa aaaaagcaga aagttcatta gaaagtttga aaaatttatc
    12301 tgcaccaaac gcaaaggtaa taagagacaa taaaaaaatg acaattccag caaaaaattt
    12361 agtagttgga gatattgtat tcttagaagc tggcgattac ataccagcag atggaaggct
    12421 tatagaagca caatctctca aggttgtaga agggatgtta actggagaat cagaaccagt
    12481 tttaaagcat acagaaaaaa taagtgaaga tgtaagttta ggtgaccaga aaaatatggt
    12541 atttagtggt gctgtagttg tttatggtag aggttctttt gtagtgactg gaacaggtat
    12601 ggatactgag atgggtaaaa tagctgggct tttagaaagt gctgaaaata aacttacacc
    12661 attgcaacaa aaaatagagg attttagtaa aaaacttggt gtaggtattg taatacttgc
    12721 tcttttaata tttataattc aagtagcaag aaattacttt atggctggaa atggagaaat
    12781 gacacaaatt attttagatt cattcatgtt ctctgtagca gtagcagtag cagctatacc
    12841 agaagctcta tcatcaatag tcactatagt attggctgtt ggaacaaata gtatggcaaa
    12901 aaaacaagcc ataataagaa aattaccagc agtagaaact ttaggttcaa caagtgtaat
    12961 atgtactgat aaaacaggta ctcttacaca aaataaaatg acagtagttg attattatat
    13021 gtatggaact aataatgatg agttagataa agaaaaagta aactattctt atcatgctaa
    13081 attattgact atggtttcta cattgtgtaa tgactctcat ataactagtg atggtaaaga
    13141 agttggtgac ccaacagaag tggctttaat aaattattca agtaaaaatg atttagatta
    13201 tgataaatta agaagtaagt atatcagaat aaatgaaata ccatttgact cagacagaaa
    13261 gcttatgtct actgtaaata gtatatatgg agattatata atgtttacaa aaggtgctcc
    13321 agatattgtg tttagtagat gtaaatatgc tttgaaaaat ggttcaaaag tagatataac
    13381 agatgagata atagaagaat ataaacataa aaatgaagag ttctcaaata gagcacttag
    13441 agtattagct tttgcaataa aagatgtccc agaagaagat tttatacctt caattgatga
    13501 cgaacatgac atgacattgg ttggaataat ggcaatgata gacccaccaa gagaagaagt
    13561 aatggatgca gtaaaagagg caaagagtgc tggtataaaa actgttatga taactggtga
    13621 ccataaaaca acagcagcag caattgcaaa agagattggt ataatggaag aaggagactt
    13681 ggctcttact ggaaaagagc ttgatgcttt gagtgaagaa caactatatg aaaaactaga
    13741 aaatataagt gtatatgcca gagtctcgcc agaaaataaa attcgtatag taaaagcatg
    13801 gcaacataag aataatataa cagcaatgac tggtgatggt gttaatgatg cacctgcatt
    13861 aaaacaagca gatataggaa tcggtatggg aagtggtact gatgtagcaa aagatgcttc
    13921 tgctatggta ttggttgatg ataactttgc aagtatagta aatgctgttg aagttggtag
    13981 aacagtatac agtaatataa taaaatcaat tacttatcta tttgcaggaa acttaggagc
    14041 tatagtatcc atagtatttg cagtgtttgc aggttggtca aatccattta cagctttaca
    14101 acttttgttt ataaacttag taaatgactc tttaccagca attgcattag ggtttgaaaa
    14161 gtcagaaaaa aatataatga ataaacctcc tagacacccg gatgaaagta tactagcagg
    14221 aggaacattg aaggcagttg ctataagagg ttgtataata ggtgtagtta caataattgc
    14281 acaatatata ggattacaag tatctccaga acttggagta gcaatggcat tttcaacatt
    14341 gatattagct agaataatgc aaacattacc tgcacgttct aataatcaaa cagtaataaa
    14401 attaggtttt ttcagcaata aatacgttat aggagcagtt ttagtctgtc ttgggcttta
    14461 ctctttaaca ctaataccag tgctaagagg aatattctct ataccaacaa cgtttggatt
    14521 gtaccagttg gggatttgcc tagggttagc attaatttca tcaatattaa tggaacttaa
    14581 caaattaata aatagaagtg aagtaaaata atcaaaaaat taaagggcat attgatttta
    14641 acaatatgcc ttttataaat agtctaattt attctatatt atcgattatt tggaatgctt
    14701 ttataacttc aggtaattct ttttcttcag gcattctaaa tcttatgaaa tttttatcaa
    14761 gtccagtaaa atcacttcca gatataactc ttatttttaa tttagcaaac tcttgttgta
    14821 aatctatgtc cttgttttta tgttcaactg tcataattga tacagtatca ctagtttcag
    14881 ctatattaag gtttttccat ggaattaata attgattttt tatatctttt gttttttctt
    14941 ttaattcttc tataaattga acatcatcta ataaatttgc tgcaatacta cgagatattt
    15001 cagacacttc gtaaggagta gaaatctttt taatagggct aacaagttgt tctggaagaa
    15061 ctgcatatcc tgctcttagt cctgctaaac caaatccttt tgaaaatgtt tttaaagcaa
    15121 ttacattatc ataattattt agaagcttga cagctgaatt ttcttttggc atgtattcac
    15181 cataagcttc atctaccata actgctatat catatttagc tgcttccttt actatatttt
    15241 caattgatga taaaggtata atttgtcctg taggattgtt tggattgtct atgtaaatta
    15301 ctttatattc tggatttatt ttttcaataa attctttttc attaaacttg aaattatcct
    15361 ctttctttaa aagaacataa tcataagttg caccatgcat ttttatatct gtctcatatt
    15421 ctgagaactg aggtgaatat cctaaaactt tatctccctt ttcaataaat agtctattta
    15481 aaaggtaaat aacatgtata gaaccatctg ccaaacaaat tctgtcagta tctaatgcta
    15541 taaaatcttt ccaataatcg actatattat cttttaaagc aattgaatga ggatatcctc
    15601 ttatcatatc aaattttaaa gcattaaaag cttctacagc tttgtttgag taagacacgg
    15661 tatttatacc ttcaccacag tctatctcga atacatcttc tgaatgtcca gattctattg
    15721 cataactttt tttcaactgc atttcaatag ctttgttaat ttttagtttc atactttatc
    15781 actccttatt tagtcaataa taaatgttat aaaagttgct agatttaaaa tattatgaaa
    15841 atatataata gtaaaaatga atataaatat tataatagct cactttattt aatatgatta
    15901 tatatcatat attgtaaaat ttctagcatt ataagactat aaattgaaaa tattcagaaa
    15961 aatactacaa gaaacgattt attttgctct taaaataagt taaatgaatg ttattaattt
    16021 tgtaaattga aatagtttaa gtataaagtt atgatataat ctaataaggg taattcttta
    16081 gattatatta aggaaatata ggtatacaat aaaaagttat ggagttggat tatgggatat
    16141 aaattaattg taacagatat ggatggaact ttattaggca ataatcataa agttacagat
    16201 gaaaataaaa ctgcactaca aaaagttata aaaagcggta ttaatgtaac tttagcaaca
    16261 ggaagagcct tcgattcagc taaatgcaat gtagattttc ttaaagagga catgccaata
    16321 atagcttgta atggttcatt aataagagag caaaatggaa atataatata ttcaaataag
    16381 attgatacaa gaacatgttt aaacatttta gatgtattag ataaatacga tatttactac
    16441 caatgtaata gtatagattc tatgctttcg aaaaaaatag aaggacgtga agatagatta
    16501 agtgtatttt taggctctga aacagaagta attgtaaaag atgatttgag agaagaaatc
    16561 tttaaaaaag atatactaaa atttgtaatc atagaagaaa aaaatccatc tattttggat
    16621 gaaataagaa aagaattgag aaaagttcaa ggaataaaga taacttcttc atggccaaac
    16681 aatattgaag tcatgaatga aggtgttgat aaaggtaatg cagttaaaat tttagctgaa
    16741 aaaatgaata tagacagaga agatataatt gcatttggag acaattacaa tgacatagaa
    16801 atgataaagt ttgctggtct tggggtagct atgggaaatg cagaagaatt gattaaacaa
    16861 gaagctgatt atgttacaga tacaaatcaa gacagtggag ttgcaaaggc aatttataaa
    16921 ttcctagagt taaaagaatc acttagctct taaaacagta tataatagga aaaaataggt
    16981 tgactatttt gtagatagta attataatac aaatagataa aacataaaaa tttagattca
    17041 gtgaaaaaga gagtagatat agagaaaatc tcagcgaatt gggggatggt gtgagcctaa
    17101 tgataacact ttatattgaa aagaactttg gagaatacta cttgaaattc agtatggtag
    17161 taggtaatct cgccttaaga gaatagagtg ggtaaacatt gtttaccaac tagagtggta
    17221 acacggaata tttcgtctct tggtcttttt gaccaagaga ttttttagtt tcattgagtt
    17281 acaagactta taattaaaat acaaaaaatt aaaatatgag gtgaaagtta tgaaattatg
    17341 gggcggacgc tttagaaaag cggaaaatca acttatggaa gaatttaata aatcttttgg
    17401 ctatgattgt gtactgtaca aaaaagatat cgaaggaagt gtagcacatg ttcatatgca
    17461 agtaaaatgt ggacttttaa cagaagaaga aggaaagtct ataacagaag gtttaaaagg
    17521 gatacttgaa gatgtagaaa atggaaagtt agtattggat ggagagtatg aagatataca
    17581 tagttttact gagataaatt taattcaaag aattggagat gttggcaaga agcttcatac
    17641 agcacgtagt agaaatgacc aagttgcagt tgatatgaga ttgtatgcta aagaaaaagc
    17701 aaatgatttg gtggatttaa tatctgaatt taaagctact ataaaagatg ttgctgacaa
    17761 aaacccagtg atgatgccag gctatactca tcttcaaagg gcacaagtag tgacatttaa
    17821 gcatcactta atggcatatt atagtatgtt tgatagagat gagaaaagaa taaaaaatgc
    17881 tatagaaata ttagatgaat cacctttagg ctgtggagct ttagcaggaa ctacgcatga
    17941 tatagataga agtataactt gtgaacaatt aggatttaaa aaagtagtag ataattttat
    18001 ggatggagta agtgatagag attatttact agagctaatg agtgattttt caattataat
    18061 gatgcactta agtagattaa gtgaagagtt gatactttgg agttctcaag aatttggatt
    18121 tgttgagata gatgatttat atacaactgg aagtagtata atgcctcaga agaaaaatcc
    18181 agatggtgca gagcttataa gaggtaagac tggtagagta tatggaaact tatttggtct
    18241 ttttacagta atgaaaggaa taccattagc atataataaa gacatgcaag aagataaaga
    18301 aggttttttc gatagtgtac atactcttga aatgtgtata caaataatgg atagaatgat
    18361 agccacacta aaggtaaatg aggataaaat gaaacaagca gtaaaaaatg gttttttaaa
    18421 tgcaacagaa gttgcggatt atttggttaa aaataatgtt gcttttagag atgcacatgg
    18481 tatagtagga agtatcgtta tatattgtga agataataaa aaagcaatag aagatttaac
    18541 attagaagaa ttacataagt tttcagatgc ttttaaggaa gatatttatg attttatcga
    18601 ctatgagagt atattaaata agggaataaa aaagaattta aaataatgta tttattttta
    18661 aaagctgtaa atttgaatgt tatcatttaa atttacagct ttttattgtg taagatttta
    18721 ttatataaaa gtaagaaact taaaaggagt agtatttaaa ataaatacaa ataaaaaatt
    18781 attattaaaa tgataattaa tatcaataag tattgacaaa
//
LOCUS       DAEMIK010000080        18779 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEMIK010000080 DAEMIK010000000
VERSION     DAEMIK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.080"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            281..694
                     /locus_tag="KPK87_002273"
     CDS             281..694
                     /locus_tag="KPK87_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2538420.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
     gene            885..2102
                     /locus_tag="KPK87_002274"
     CDS             885..2102
                     /locus_tag="KPK87_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG2538421.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            2264..2962
                     /locus_tag="KPK87_002275"
     CDS             2264..2962
                     /locus_tag="KPK87_002275"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG2538422.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(3182..3799)
                     /locus_tag="KPK87_002276"
     CDS             complement(3182..3799)
                     /locus_tag="KPK87_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG2538423.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(3926..4948)
                     /gene="argF"
                     /locus_tag="KPK87_002277"
     CDS             complement(3926..4948)
                     /gene="argF"
                     /locus_tag="KPK87_002277"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG2538424.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(5057..6244)
                     /locus_tag="KPK87_002278"
     CDS             complement(5057..6244)
                     /locus_tag="KPK87_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG2538425.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(6274..7134)
                     /gene="argB"
                     /locus_tag="KPK87_002279"
     CDS             complement(6274..7134)
                     /gene="argB"
                     /locus_tag="KPK87_002279"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG2538426.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGEIYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            complement(7149..8366)
                     /gene="argJ"
                     /locus_tag="KPK87_002280"
     CDS             complement(7149..8366)
                     /gene="argJ"
                     /locus_tag="KPK87_002280"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG2538427.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(8407..9441)
                     /locus_tag="KPK87_002281"
     CDS             complement(8407..9441)
                     /locus_tag="KPK87_002281"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG2538428.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(9695..9908)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10211..11302)
                     /gene="ytvI"
                     /locus_tag="KPK87_002282"
     CDS             complement(10211..11302)
                     /gene="ytvI"
                     /locus_tag="KPK87_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2538429.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFAIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     gene            complement(11317..12708)
                     /locus_tag="KPK87_002283"
                     /pseudo
     CDS             complement(11317..12708)
                     /locus_tag="KPK87_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
     gene            complement(12727..13266)
                     /locus_tag="KPK87_002284"
     CDS             complement(12727..13266)
                     /locus_tag="KPK87_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2538430.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLVIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            complement(13287..14612)
                     /locus_tag="KPK87_002285"
     CDS             complement(13287..14612)
                     /locus_tag="KPK87_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2538431.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIKKQKKYHY"
     gene            complement(14609..15232)
                     /locus_tag="KPK87_002286"
     CDS             complement(14609..15232)
                     /locus_tag="KPK87_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBG2538432.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELEIKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            15324..16133
                     /locus_tag="KPK87_002287"
     CDS             15324..16133
                     /locus_tag="KPK87_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2538433.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISIQVI"
     gene            16567..16929
                     /locus_tag="KPK87_002288"
     CDS             16567..16929
                     /locus_tag="KPK87_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538434.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(17066..17185)
                     /locus_tag="KPK87_002289"
                     /pseudo
     CDS             complement(17066..17185)
                     /locus_tag="KPK87_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(17279..17836)
                     /locus_tag="KPK87_002290"
     CDS             complement(17279..17836)
                     /locus_tag="KPK87_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2538435.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            18094..>18779
                     /locus_tag="KPK87_002291"
     CDS             18094..>18779
                     /locus_tag="KPK87_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2538436.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNT"
BASE COUNT         6512 a         3130 c         2211 g         6926 t
ORIGIN      
        1 aatagaataa catctaaaat agtttttttg tattaaatta acttatttta atattcaaca
       61 atttaggttt actaaattta ttgatgattt ctttttatat aacttaaact acatttttat
      121 ttaaaaaaat ttaatatcaa caatataaaa ttaagaataa gtattattag tttgacatta
      181 gcataaatgc ctattagaat aatgttaaac tatattcttt ctactgattt tattaaaaaa
      241 taccaatata caaaagatta tacaaggaga aagttcaaat atggaattag aaattacaga
      301 aaaaatagaa aaacaagatg aagatactat ttttcaagga ttattaaaat ataatcttgc
      361 tagacttgaa gataaaaacc cagtggattt aggaatatat attcgagata aagcaggtaa
      421 aattgttgca ggttttatag gtgttactca tggtaattgg ctttctataa aatacttatg
      481 ggttagtgag aagcttagat ataaaggtac tggaagtcaa cttttatata aggctgaaaa
      541 aattgctaaa gaacgaggtt gtaaatatgt ttttttagat acattcagtt ttcaagcacc
      601 taaattttat gagaaatttg gataccataa agtattttca cttgaaaatt atcctctaac
      661 tggaaagaga tattattata gaaaaatact ataataataa aaatcatatt tcatattttt
      721 aaattaaaac ttctgctctt cgtctacaga ctttatgttt ttatatcatt attaatacta
      781 aaatagtatt ttactataca tacaaaatta tttgaaagtt caaaaattat aagtataaaa
      841 gctaatctta gcttaaataa aaattatatt tttaggaggt aaatatgtta ggaaaaaaat
      901 gtatggatta tctacaaaca ttaggaaaaa taagttcaac aacaaatggt cttacaagac
      961 ttatcttgac tcaagaacat aaaaaatcta ttgatttaat tagttcatgg atggaaggtt
     1021 taaatcttga tattgaaatt gatgacattg gaaacgttat aggtacatat aaatcaagct
     1081 ttccaaatgc tcccacatta gtagttgcat ctcatcaaga tagtgtaaag tgtggaggta
     1141 tatttgatgg tatgttaggt ataatagtgc ctctagttgg tcttgaagaa gcaaaacaca
     1201 ataataggtc ttatcctttc aatattaagc taattgcttt cgctgaagag gaaggaacta
     1261 gatttgaaac ttctttaatg ggaagtaaag tttttgctgg tacttttaaa gaggaacttc
     1321 ttaaatctgt tgatgaaaat ggaattacac ttgaagaagc tgtaacaaag tttggattta
     1381 atacaaaaaa cttaacaaat ttgcatccta gaaaagatgt agatgcatat ttagaatttc
     1441 atattgagca gggtcctgtt ttagaaaatg aatctcttcc tgctggaatt gtatcaagta
     1501 ttacaggatt taaaagtttt aagatatcag taaatggtaa gtctggacat gctggtacac
     1561 ttccaatgaa tatgagactt gatgctggat gttgtgcctg tgaatgtgta ttagctatcg
     1621 aaaaagtagc taaaacgact actgatttgg ttgctactgt aggaaagatg aatttctatc
     1681 catcttcatc aaatgtggta cctgaaagag cagaatttac actcgatgta cgctcttgtt
     1741 cacaagaaat tcttgataat tctgtagaaa agatatttaa tgaaatatca catatatgtg
     1801 aaaatagaaa acttaattat acaagtgaac ttgcttttga aaatgttcct gtaccatgtt
     1861 ctaataaaat aacaaaaatc atagaaaaaa gttttataga tttaaatctt aatccttttt
     1921 atatttatag tggtgctggc catgatgctc aggaaatgga taatataact gatattggta
     1981 tggtatttat aagatgcgct gggggcgtaa gtcataatcc taatgaaagt gtctctgtag
     2041 atgacttgga tactgctgta aaaatatttt taaaaatact agataatctt gatttaaaat
     2101 aattcttata taagaaagta tattaaaata tataatctaa aaaatcactt tatatttata
     2161 ttatataagt gtacaatata atctattata aaatataatt ttatgctttt atagattttt
     2221 attttataca taaatatatt tatatataaa ggagataatt tttatgaatg aaaaattaat
     2281 tggtattttg gctggaatgg gtccaaggtc aacagcccct tttgttgatt taattattga
     2341 tgagtgtcaa tctcaatatg gagcaaaata tgatgatgaa tttccaaaga tgatgatata
     2401 ttcattacct accccatttt atattgaccg tccaataaat catgacctta tgaaagaaac
     2461 tataattgat ggattacaaa agcttgaatc aactggtgta aattttattg caatgccatg
     2521 caattcagca catatatact ttaaagaatt aaaagagtct attaatatac caatattaaa
     2581 tattgtagaa gaaacagtta aaaaattacc tactatctca caaaaagtaa ctcttttttc
     2641 gactagttca acttttgagt ctacaattta ccagaatggt ataatacata atggacatga
     2701 atttatattt aaagatgaat ggcaaataaa attaaacaat cttattcaaa atataaaaat
     2761 ggataaagaa aatccacaca atattgatat ttggaataaa cttattgaag atgttaaaaa
     2821 tgaatctata gaaaatatag taattgcttg tactgattta aatgtagttt tagaaaaatc
     2881 atatccttca ataaatattg ttgattcatc aaaatgcttg gctgaatcag ttgtcaataa
     2941 atatctaaaa ttagttaaat aaatttttca tacactatat tataaaaaat atatgttata
     3001 agtttatttt aaatagtata gacttataac atatatcata gtttcttgac aatatagaaa
     3061 ttaaccatgt atttcattat attaataaaa aggataccaa aaatggtatc cttcataaat
     3121 atttatatgt ttatttataa aaataatcat atatttatta ctttactaca cctaaacaat
     3181 attattcaag tgtattcata gagttagaaa attcttgtcc tgctgtaaca agattagata
     3241 acaatacatc agctactgca tcatttactg aacttattat ccatttatta tcctttttag
     3301 aacattcaaa ctctacattt tttgtaacat aagtctcttt aacgcttttt tgtttttctt
     3361 tcataattga tactaacatt tcagttgttt tctcatcagt taagtcttgt cctgaaaaac
     3421 tcataccaat catctttgta aatgcctcag ctactatttc ttgaagtaat ggtgttgaat
     3481 ctataaattt acattctact ttgattgtag ccttatcacc tttaacctcg ctgtctttta
     3541 ttgtatatgt gattttagat gcattctgtt ttagataatc taaaacatat tgggcatttg
     3601 ggtcttttgt atctaattcc tttaatatat ccttatattt ttcgtcatta ttttccatta
     3661 ccttattcat gccttcaaag tcaaactttt tcgcactgtt aaaaaaacta tcaattgtat
     3721 cttctggttt tgcacttgaa catgctgtta aagatattgc taataaaaaa gttgttaaag
     3781 ctaatatgat ttttttcata tctcaaatcc tccctcttaa tttgttgata cataagttat
     3841 tttaacatat aatgagagtt tttgaataat atatttaaaa tattttataa attttcatta
     3901 aagtaaagtc tatattaaaa catatctata ttaaattatt gttcatattt acatcagtca
     3961 tagtagaaac cataacagct tttattgtat gcattctatt ttcagcttct tcaaatacct
     4021 ttgaatattt tgaattaaat acctcgtttg taacctctaa ttctgataat ccgaactttt
     4081 cataaatctc ctgagcaacc ttagtttcta aatcatgaaa tgctggaaga cagtgtaaaa
     4141 atataacatt ttcattttca gttttcttta tcatatccat atttacttga taatctttaa
     4201 gttgatttat tcttgcttca tattgctcct cttctcccat agaaacccat acatctgtat
     4261 atataacatc tgcaccttta acatcatcaa tattttcagt caacattata tctcctttgg
     4321 atttcttggc tatttccttc atttccttaa ccaattcttc atttggccaa agttccttag
     4381 gtgctagtgc tacgaaatgt attcccattt tagcacatcc tatcatcagt gagttcccca
     4441 tattatttct agcatcacca acatatacaa atttaacttt attcaaatct ttatctacat
     4501 tttcaattac tgttaaaaaa tctgccagta tttgtgttgg atgatattca tctgtaagtc
     4561 cattccaaac aggtacacca gaatattttg ctagactttc aactgtttct tgtttaaagc
     4621 ctctaaattc tattccatca tagaatcttc ctagaacttt tgctgtatct gatacagact
     4681 cttttttacc catatgacaa tcatttggac ctagataagt tacatgtgct ccttcatcat
     4741 gagctgctac ttcaaatgaa catctagttc ttgttgatgt tttctcaaat aataaagcta
     4801 tattttttcc ttgtagcaaa tcgcctttta taccaacttt tttcttagtt tttaaataac
     4861 ttgataaagt taataaatat ctaatctctt cttttgtaaa atcctttaat gtcaaaaaac
     4921 tcttaccttt taaacttata ttattcatat taattcgccc cttcttataa actttttaaa
     4981 ttcaattaaa cttgttagca ctttactttt ttatttactt ctaattttta tctttaatca
     5041 actatttttt gaatatttag ttaatttcta gtaatatatc ttttaggatt tcaagagcct
     5101 catctatttc cttataactg attgttaatg gtggtaaaaa tctcaataca ttttttccag
     5161 cagtcaaaat aagcaatcct ttttttctag ctttctcttc tataatacta gattcaatat
     5221 tagtttcaat tcctatcatt agaccaatac cttttgtctt taatataact ttgttattaa
     5281 aactttcaat agattttctt atataatttc ctttttctaa tacttcatta taaaagccat
     5341 ctttagatat ctcatctaat acaactgaag ctgccaccat agctaataaa ttacctccaa
     5401 aagttgtacc ttggtctcct tttcctagta cttttgaaag ttttttattt accaatactc
     5461 ctcctactgg tattcctgct cccaaccctt ttgctacggt aactatatct ggttttacat
     5521 caaaatattc atagccaaaa agtttaccag ttcttcctat accgcattgg acttcatcaa
     5581 aaatcactac aatatccttt tcttgacata tttttactat ttccaaaaca taatcttttt
     5641 caagaacatt aactccacct tcgccttgaa tcgcttcaag aattattgca caaactgtgg
     5701 aatcaagatt attttttaaa tcttcaatat tatttctttc tatatattta aaaccttcgg
     5761 gaagaggata aaaatactta tggtatttgt ccataccagt tgccattaaa gacatcattg
     5821 ttctaccatg aaaagagtct tttaaactta tgatagttcc tctaccatta ccatatttgt
     5881 cactactata ctttcttgct attttgaatg ctgtttcatt tgcttctgtt ccagaattac
     5941 aaaaatatac tttagccatt ccacttgaat taattatttt ctctcctgaa tacaacactg
     6001 gctcattaaa atataaattt gaagtatgta ataatttatc tacttgctct tttagagcat
     6061 tctttaaatt actattacta tacccaagag aagatactcc atatccactt gtaaaatcta
     6121 tatatttatt gccattagta tcaaaaaaac aacttccttc accataatct atgacaaaat
     6181 tgggttggtt atatgtatct ataaaatatt cattccattt tgaaattgta ttgtcgttat
     6241 tcattcttat tctcccctct taaacttata attttattca gtttcttttc ttattaatgt
     6301 acctattcca ctatcagtaa atagctctgt tattatagaa tgtggaactc ttccatccaa
     6361 aatatgtacc ctatttactc cgttatttaa ggcatctacg catccctcta ttttaggtat
     6421 catacctcca gtaattatac cttcttcaaa taatttatca acatcctcta atataacttc
     6481 tgttattaat gatttttctt catctggctc tcttaacaaa ccagggacat ctgtaagaag
     6541 tatcaattta tctgcattta actctttagc tatagcagaa gctgctgtgt caccatttat
     6601 attataaatc tctccatcat ctccaacacc tatagttgca attactgaaa tataacctga
     6661 ctttagacaa gattctataa gctcaacatt tacttttttg atttctccta caaatccaag
     6721 ttcataattt ttatatgggt cacataaaat catattatta tctattccac aaagccctac
     6781 tgcttttcca ccttttgtat gtattttatt tactaagtct ttattaacct ttccagcaag
     6841 taccattttt acaatttcca tggtctcttc atctgtatat ctaagaccat ttacaaattt
     6901 actttctata tccacttttg ctagcatctt attgatttct gcccctccac catgtactaa
     6961 aactatattg attccaacat aactcattaa cacaaggtct tccataacag attcttttag
     7021 accatctttt ttcattgcac ttccaccata cttaactaca atagtctttc cctgatgttt
     7081 ttctatatat ggtaaagctt ctataagtgt atttgctttt tctatattaa tcattaaagt
     7141 atatcctcct aatttctata acttccattt attcttacat aatcatagct taaatcacat
     7201 ccccatgcat ttcctctggc attgcccata tttaaatcta ctaatatact tatttcgtca
     7261 tcttcaagga ttttttttgc tttttcctca ttaaaatcta atccatttcc atttttacat
     7321 acttcaatat atcctttcat actctcaaaa ataatatcaa ctttttccat atctatttct
     7381 tcacctgcat aacctaaagc acacagtatt ctaccccaat ttgcgtcagc tccaaataca
     7441 gctgtcttta ctaatgaaga agatataact gtttttgcta attttactgc atgttcttca
     7501 cttaatgctc catttatata gcattcaatt agttttgtac atccttctcc atcttttgca
     7561 acaagtttag caagttctat acagacatat gtaagtgcac tcaaaaatac ttgataatgt
     7621 tcatcttttt tgtttattct atcatttttt gcttttccat ttgctagaat cattaccata
     7681 tcattagtac ttgtatctcc atctacacta actctattaa aacttttgtc aacagcaatt
     7741 tttaaagctt cttttagcaa aactccatca atatttgcat ctgtagttat aaatcctaac
     7801 gttgttgcca tattaggatg tatcatgcca gaaccttttg ccatacctcc aatagttatt
     7861 tttttatctc catacattat tgctactgct atttcttttt ttataatatc agttgtcata
     7921 atagcttctc ttgctccaat atgaccttgt ttacttagat tattcactaa ttcatccata
     7981 ttttcttcta ttaaatctat atttaatggc tttcctataa cacctgtaga tgcaaccaaa
     8041 acatcacttt ctttaatatt taatttatca ctggtgaatt tagccatttt ataagcattt
     8101 ataagtcctt cttttccatt aaaggtatta gcattagcac tatttactat gatagcttgt
     8161 gcttttccat ttttaatgtg ttcctgtgta acataaacag gattgccctt tactttattt
     8221 tgagttacta ctgctgctgc atcacataaa acatctgaat aaaccaaagc taaatctctt
     8281 ttttctttat tttttctcaa gccacagtga atccctgaag caaaaaatcc ttctgaaact
     8341 gtaactccac cctttataat ctccatattt atcctcctaa ttatatgagt ttagtatttt
     8401 ttatttctaa aatgatggtg caatattttt taaacctgta ttttcttcaa taccaaatat
     8461 tatattcatg ttttgtatag cttgacctgc tgctccctta atcatattat ctatagctga
     8521 acagattatc aatgtatctc cattttcatg taatgatatt acacatttat ttgagaatct
     8581 aacattcttt aaagaagcta ccttatctaa tggaagaatt tctataaatt ctttaaattc
     8641 ataataatca gttaatttcc tatgtatatc atttatagaa atattatctt cttttgtaca
     8701 atatatagtt gataagattc ccctatttac tgggattaag tttggtgtaa aaactacact
     8761 tactttttct ttgcaggatt cacttaaatt ttgttctatt tctggagtat gtctgtgttt
     8821 ccctatttta tatgcagtga tattttcatt aacctccgta aagtgactac taatcgataa
     8881 ttctctacca gcacctgtaa gacctgattt agaatcaatt attatattat tattttttat
     8941 caatttagaa cttagtaatg gcatcaatgg taaagaaata ctagttggat aacatccagg
     9001 atttgcaatt atatctgcat cttttatatc ttctttgtat atttcactta atccatatac
     9061 agctttttta tgtaagattt tatctataaa agatactcca taccatttac tatatacttc
     9121 ttcatctttt attctaaagt cagcacctaa atctataact ttctttttcg atttaattgc
     9181 ttttattaca aacttttcac taactccatg tggaagggct gtaaatacaa catcacacat
     9241 atctataact ttttcatttt ctatacatat catgttattt ttatatccta tacttggata
     9301 tatatcaact atatcttttc caacatacga atttgaacct atcgctgtca cttcgacttt
     9361 atcatgattc attaataatc taatcaactc tgccccgaca tatccagttg caccaataat
     9421 accaactttt attttattca ttttgttccc tccattttta tattcaaata gtcttaaaat
     9481 cctatctttc cactttacta tatgataatc atttgataat acgtagatta aaatactcta
     9541 attaaaacaa aaaacccctt agcattataa atactaagag gcgattttcc gcggtaccac
     9601 tcttattggt aaaattacaa taatcattaa aactcaaatt aaaaagtatc tacaaaatgt
     9661 acatttaaaa ttaaaaaagc ctcttggtca aagaccaaga gacgaatata cacccgcgtt
     9721 accactctaa ttgataagat aatcttaccc actctaatct cttaaggcga gattcaccaa
     9781 ttaccatact attacttctg gtaatctcct ccaaagctct attcacaata aaattcgtta
     9841 ctaggcttcc accattccca gctctctatc aactagcttt atgctactct ctttttcaca
     9901 ggatttcaat ttattttctt attgttttat atgatagttt atttttttaa ctttgtcaat
     9961 ataattttta aatttttgga aatattttct atataattaa tttccagcat tttgtatcta
    10021 aattgagtta taaatatcta cagtaatagg acaccatgaa tttcctgctg taatatattt
    10081 taaaaaagtt ctccctcttg aatcaagtca ttcaaaagct atacctttgt caaaattatt
    10141 tttcatccag cgttttttag aaaaaacaca attttctcca taaaagagac atacatacaa
    10201 ctattctttc ctaatgtaat atatgtatga tttctttgtc atttaacttt ttcaaaaacg
    10261 caattaacaa tggaataccg aacaacccaa taaatccaaa gaaatgtaat cctgttagca
    10321 tactggctaa ggccaataca ggatgtaact ccatatgttt accaactaat tttggttcaa
    10381 taatattcct gataatagta atcacaatat atagtagtgc aattcctaat gccattgaat
    10441 aatcccctaa aaataaactg ataatacacc aaggtattag aatacttcca gttcctaaaa
    10501 caggtagtat atctaaaaga gcaatgaata aagcaattat tccagcataa gaaatattaa
    10561 taatagcaaa accgacatat aattcagaaa aagtgatagc aaaaattaaa agataagaaa
    10621 aaccacattt aataatggta tcaatagtaa acgcttttgc ttctgctaaa agataacttt
    10681 tatttttagg tatctgtagc actataaatc tctttatcgt tggataatca attgctataa
    10741 aaatagaagc aacgattgta ataattgttt tcataaatag taatggaaca gacgaaacaa
    10801 tatcagtaat accagatacg atattatttg aaagagaaac tatgaaaaca ctcatttcat
    10861 tcatcaatac aggcatattt gcttctatta attcaataaa cgaaaaattg aatgaaccag
    10921 aaaatagttc gacttctttt ataactgttt cagtcaaagg tattaccaat tcctcaaaca
    10981 tggttggtag gaaatgaaaa atttccttta ttccatatat tagatatgta cctattacac
    11041 ttactattat tcctgcaatt aaaaagaata aaaaaacaga tatggttgct acaatacctc
    11101 gctttattag taatttttca cttatgtata agactggttt attcaataaa cttgctatta
    11161 aaaatgcaaa aacaaaagga agtaatacag aaaacgcata tttccctaat aacattagta
    11221 agcctaatat cactaccaaa taacagaaat ttattaaaaa tctctgctta ttttggtatg
    11281 atgtatgatc ttgttcattc aaataaaatg cacctcctac tttatagtaa ctttatatgt
    11341 gccttttgga gcaacagggg gcattgtttc acacattcca gaacaacttt ttgcttgcac
    11401 aaaatattca atcatatcac ctgactttcc tatcatattg gcaaaaaata tttctgcttc
    11461 tgtagtttct tctaacataa tactttccca tgtttgaaaa ccatagtatc gataaaacag
    11521 tctacaattt tcataatcta agccagcttt actataatca actatttgaa tagtaacttc
    11581 taaagcatca ttctctgaaa cgatttcttc taatcttttc acggaaatat agagcatatt
    11641 ctcatcccaa attgctcttg tcctgcaatg aataacatct tcatttcccc agccagttct
    11701 cacatatcct gtacgatttt ttataatatc tggctctttt tcaaaatcaa actcatatcc
    11761 tttaatttca tatccaggca aagcgttctg ccattgtttt attgcaattt tatcctgttc
    11821 aattccatac attggaacat agacacgact atttaaaatc acagaattaa cataagcagt
    11881 aagttcatca ctgtcctttt gaaacggaac aatatctata cgaaagattt tgaatggacg
    11941 tccatatctg gtttctacct tttttacttc ttctaaaatt ttttcatatc tttcatacaa
    12001 atagtgttct tttggcgttc tgctcacaag taatgtttct tcatcaatta tttttaaata
    12061 acagtcaata tgttgtaaac cataatcagc aaaattagaa aatatataat actcattcat
    12121 tccagtatct cttgccacca tagaaaaaaa ttcttcgtca gaaattcctt tcgcacgatt
    12181 ttctgtctgt aaaatgtgca tagacatcaa tttattgaaa ccatctgaca taagatttcc
    12241 gcctgttagt gcataaggta atttcataaa tggcaaacca atctgtttcg caatcaactc
    12301 ttgtgattga tcttcttttt gttcataaga gttagtagtt aataggttat gttcgcaatc
    12361 aaacatgatt tctggtttat ttatggatac aaatggtgtt gacatcttat attctgcacc
    12421 agccaaaaag agttttccgt tttcatcaaa aatagggtgc ataccccaat cacgaaccca
    12481 tggtgtatca aatccctgtg gagcaatgca gaaaatcaaa ttttttaaat ttccacccca
    12541 cttacttaaa taatattttg caatttcaat ttcctcttta tttggtacac acatgtatac
    12601 agaataattt tacgttaaat ctacaatata cttatgggga agaaaagggg gccatgcaag
    12661 catgacacct ttaatcggtt cccattcccc agctaaacgt cttttcattt ctaatacctc
    12721 actttcttac tttttccaca aatgaaatgg aatttctcta attttaagat ttttcaacaa
    12781 tgtttcaaat tcattttttt gaaaggtttt ctgattacat aaaacttcca tttctagtcc
    12841 cttatactcc atgagatatt ctgtttcata aaacccattc tgaagataaa attgcttcct
    12901 taaaattctt tgctgtaaat taggagcact gtcctctata gtttccatat caagaacaat
    12961 ttgacaattg ggatattgtt ttttcataag tgacagtgct tttccaccat atcctttaga
    13021 gcgttgttct ggagaaattg caagaaataa tatatatcct atatgatttt caatcaccaa
    13081 aacataaaaa ccaataaatt gttgcttatc atataaagca gatacttcta tttttttact
    13141 ttgaactaaa ttaagcattt cttgtataga catacgttct tcatctggaa atgcctcgtt
    13201 attaagttct tcaaattttt ctaaatcttt aaaatatgaa gttacttttt gtattgttaa
    13261 gctcatatag atacctccaa attgatttaa taatgatatt ttttctgttt ttttattata
    13321 aagaaagccg tcataatagt tgccaatatt tcagcaccaa caatagaaaa ccatatccca
    13381 tttactcccc agaatatagg gaatataagt actgatacaa cttgaaatac taaaatacgc
    13441 ataaaagcaa taagcgcaga agttaaaccg tcatttaatg cagtaaagaa actagaacca
    13501 aaaacaccaa aaccagaaaa taaaaaggaa aaagagtaaa tcataaatgc tcttaatgtc
    13561 atttccatta aattattatc gtaacccaca aaaataaaag caatcggttt tgataaaaat
    13621 tcgcctatta taaacataaa tacagcaaaa caaataatta caatcaaact tctttttctt
    13681 aaatttttta attcgtcata attttttgct ccataatgat aactgattat aggggcagta
    13741 ccaacagaat acccaataaa aacagcttga aatagtaacg ctacataaag aagaacacca
    13801 taggatgcaa cgccatcttc gccagcatat tttagtaatt gagcattata cagcatactc
    13861 acaacagatg tggaaacatt agtcattagt tctgaagaac catttgcaac agtctttttc
    13921 aaaacatcac aatcaaattt aggtttctta aatttaagca aactgttgtt ttgtcggcta
    13981 aaataaagta atggaatgat accaccaaca tattgaccga ttgcagttgc cagtgctgca
    14041 cctaccaatc cccatttaaa aacagtgata aataatgcgt ctaaaacaat atttgtaatt
    14101 ccagccataa ttgcaacata taatccaagt tttggctttt ctgctgttgc aaataaacac
    14161 tgaaattcgc actgcaaaat ataggcagga atagcgataa ttaccacaca tccataaaga
    14221 atactatctg tcaataattg accatctgct cccatagcag aagcaatcca tggcatagtt
    14281 actataccta aaacagctag aataatacca caaccagcag aagtaacaat gagtaacgaa
    14341 aactgttcgt ttgcttcttc tttttttcct tcacccattg ttttagcaat taaagctcca
    14401 ccacctgttc caaaaagaaa gccaacacac cctaagatta gcaaaaatgg cataataaaa
    14461 ttgacagcag taaaagcagt tttccctaca aaatttgaaa caaaatatcc atcaacaacc
    14521 ccataaatag aacttattac cagcataata attgtaggaa atgtaaattt taataagcgt
    14581 ttataattaa aattatctga caactgtatc attgttcata cctcctaaac caatactctg
    14641 aaaagttagc aattgcataa gaaagatgtt ttatattcca ttcagatgta ttaactataa
    14701 tatgatagga agtttgattt ccccataagg agctcgtaaa aaattctctt gatcttgctc
    14761 gatttttatc aatctctctt atctttattt ctaattcttt ttttgaaata tgttcattgt
    14821 ttaacttttt ttcaatacag cgttgtattt taaagttcat atttgcacaa acaaaaacgt
    14881 taaatggctg gtacttcttt aataatatat ctgcatttct accaacaata acacaatcct
    14941 tacctgtact ggcaatctga ttgataacat ttgattgttc tattaacaaa tctgtcttca
    15001 tggattgcat agacaaaata ttagtaaaag aagtacacat tgttaaagga acgtttctcc
    15061 aaccatgatt ttctaatatg taggaaacat agttttcgtc aatttttttc tttctagcta
    15121 tagcagtgat aatttcttta tcgtagtagt caaaatttaa aatatcagca agttgttttc
    15181 caagttctcg tccaccactt ccaaactctc ggctaattgt aataattttc ataatatctt
    15241 ttcctcacat ttcattgaat ataaaagctt tctatgttat cctaaagtat ctggtaaccc
    15301 taaagtcaat aggagattta aaaatgatta aaaaattata taagacaagt gaatttgcag
    15361 aattgtgtgg ggtaaaaaaa catacacttt accattatga tgaaattgaa ttattaaaac
    15421 cgagtattac ccatgaaaat ggctatcggt attatacaat tgaccaattt gaaaaattta
    15481 atattatttc tgtgctaaaa aaagcaggta cacctttaga agaaataaaa caatatctca
    15541 atgatttaga tacgaatacc tttcttaatg tacttcacag aaagctaatg gatcttcaag
    15601 aagaagtgaa gaagattgaa aaaatgtgtt ctatgttgag aaatgtcatt tcaacaatgg
    15661 aaatacatct caatgtaaac acagaaaaaa tcgaaattgt aaattgtgag gaagaatatc
    15721 tgattgcaac taaaattcct gaagctgtac taacagatga aaaaacaatg ttacaaggta
    15781 ttggaaatca cttacaatat tgtttattac atgattataa ttttcatgta ggagaaattg
    15841 ttcttgaaaa aaatataaaa cagggaattt ttagagagag ctattattat agccttatta
    15901 aaaataaagt agatgatgag cgccttttta ttaaaccaaa gggaaattat gcagttatgt
    15961 atcaccaggg gagttatgac agtttatata aaacttatca aaatatgaaa aatcagttag
    16021 aagaacaagg gtatcatatt gttggaaata tttatgaaga agatttaatt gattatttat
    16081 cagaacataa cgccgaaaac tacattcaaa aaatttctat acaggttatt tgaaaatata
    16141 aaaagcgtgt atatctcaaa tcacatgaga aatacacgcc ttttttaata tctcgtattg
    16201 tctataataa tttgcttaaa atgtcctttt ttatcttcta aagttggtat cttatttcca
    16261 aatttttgta gaaattcagg ttcattgcta ttatttgttt cctcaatgca aactgcattt
    16321 cctcatgaat ttcagataca gaaatagcct tttattttcg ttaaccattt aatcagtttt
    16381 ttactatctt tttataaaat cttaacatgg aggctttatg cctccatttc ttttgtagtt
    16441 gtcttaaaag tagtgcaaat ttttttctta aatatatatt atataaatta ttcatttata
    16501 tagagtatta tattatgctc tgtaaatagt tacaatattt aaaagaatga aaggagaaca
    16561 aaaactatgg aaattgatta taaagcaatc ggacagcgaa ttaaaattgc aagaattaaa
    16621 aaaggtataa cacaagaatc cgttgcaaac attatcaaca tgacaccatc ccatatgagc
    16681 aacgtggaaa caggaaaaac gaaagtcagc ctgccaacct tgattgcgat tgccaatgca
    16741 ctttctgtat cagtcgatac tctcttatgt gataatgtac tttcttccaa agtaattttt
    16801 gaaaaggaag ccaaagatat tttctcagat tgtgatgaat atgaaatccg ttctttagtt
    16861 gaactgctaa aatcagcaaa aactgtaatc cgtaaagaca gagaaatccg caaacaattt
    16921 gaaaaataaa accacagctt caaggtataa attgatatgt gttgttagct aaggtctttt
    16981 ctttttttat ataatcattt ttatataatc aaataaccta tccaataatt ttccaattac
    17041 tgtccttatg aaatatcaat ttatactatg atacctgtgt cgcttttgtc tgattatcaa
    17101 ggaattttac agctaccttt tcattatcaa cgacaacagt aaaaataggg tttaccaatt
    17161 cagaaagtaa atattcttga aatatactat tttcctttca taaaaatgat aaaattttat
    17221 tttacatccc acgtaatgaa gtgttcaata aataagttaa aataatttat ttacctcctt
    17281 aatcatattt tttatgaata cctaataaca cagtatcaat agaatcgact agacctttta
    17341 tagcttcttc tttactcaaa agtgatttcc caagttgcaa agtcgaataa ccatgaagta
    17401 cactcattat taagtttaat acttcacctg tattctttgt atttagatta caagaaagaa
    17461 taagtttttc taataaggat ttatattcat caaagatttc taatgtttca ttatttccat
    17521 gccaagtagc ccactgaatt gtttcataaa caccaggatt agaaattata aaattaaagt
    17581 atgctatact tatacacttt atagctgtat ctccaaaatt tccaatagcc gcctttatca
    17641 tttgactatt catctctctc attcctttat gagctacttc aagtaataaa tcatctaaac
    17701 tttctatatg attgtacaaa gatggtgtac gtattcctag cttttctgca ataactttta
    17761 aagaaacttt gttaagccca tctttattaa ctatatctga tgtagcttgt ataactattt
    17821 ctctactaac acgcatgatt ttccctcttt catttaaaat taattacttt attagtttag
    17881 ctaatctaat tagtttcgtc aattattgta atttataaaa ttctttatac tattataaaa
    17941 tcttgacagt ttaattataa tttaatataa tactaattga attagtttta atactaatat
    18001 aattagtatt atattaaatt ctatattaca acattatttt atagtttgat taatttaaca
    18061 atcactaata tatcaaactg aaaggagatt tttatggaaa aaaaatattt cgaaattcat
    18121 ggagttcctt caatcctttg gggaaatcct tcaagtaaat tatatattta catacatgga
    18181 caatgtggca ataaagagga ggctgaagct tttgctaata ttgcagttcc acatgagtgt
    18241 caagttttaa gtgttgattt gcctgaacat ggagaacgta aaagagaaaa aaattcattt
    18301 aacccatggc atgttgttcc agaattagta tctattatgg aatatgcaaa gtgccactgg
    18361 aatcaaattt cgttatatgc aaatagcatt ggagcatggt ttagtatgtt aagctttgaa
    18421 aaagagaact taagagagtg tatttttgta tctccaattc tagatatgca acagctcatt
    18481 tcaaatatga tgctttgggc aaatgtatca gaagaacagc ttaaacatga actaattatc
    18541 ccaacttcat ttgggcatac tctttcatgg gaatatctac aatatgtaaa acaacatcct
    18601 atcactcaat ggaatgtacc aacaaaaata ttatatggta aacatgatga acttactgaa
    18661 atcggtgttg tagagaaatt tgtagaaaag tttaattgca accttactat tatagaaaat
    18721 ggcaaacatt ggtttaacac accacaagaa ttaaattcat tatatgaatg gttaaatac
//
LOCUS       DAEMIK010000081        18717 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEMIK010000081 DAEMIK010000000
VERSION     DAEMIK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.081"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            357..1517
                     /locus_tag="KPK87_002292"
     CDS             357..1517
                     /locus_tag="KPK87_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG2538437.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1860..2021
                     /locus_tag="KPK87_002293"
     CDS             1860..2021
                     /locus_tag="KPK87_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538438.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2214..2843
                     /locus_tag="KPK87_002294"
     CDS             2214..2843
                     /locus_tag="KPK87_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG2538439.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            3082..4341
                     /locus_tag="KPK87_002295"
     CDS             3082..4341
                     /locus_tag="KPK87_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG2538440.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSNGKVIKKEHISNSSYKELKK"
     gene            4599..5594
                     /locus_tag="KPK87_002296"
     CDS             4599..5594
                     /locus_tag="KPK87_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG2538441.1"
                     /translation="MYEEVTGLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5840..6907
                     /locus_tag="KPK87_002297"
     CDS             5840..6907
                     /locus_tag="KPK87_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG2538442.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDVMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKGKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7441..9351
                     /locus_tag="KPK87_002298"
     CDS             7441..9351
                     /locus_tag="KPK87_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG2538443.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9414..10037)
                     /gene="ytaF"
                     /locus_tag="KPK87_002299"
     CDS             complement(9414..10037)
                     /gene="ytaF"
                     /locus_tag="KPK87_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG2538444.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10225..10869
                     /locus_tag="KPK87_002300"
     CDS             10225..10869
                     /locus_tag="KPK87_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538445.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            11083..12141
                     /locus_tag="KPK87_002301"
     CDS             11083..12141
                     /locus_tag="KPK87_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG2538446.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12245..13429
                     /locus_tag="KPK87_002302"
     CDS             12245..13429
                     /locus_tag="KPK87_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2538447.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13636..14349
                     /gene="rpsB"
                     /locus_tag="KPK87_002303"
     CDS             13636..14349
                     /gene="rpsB"
                     /locus_tag="KPK87_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG2538448.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14428..15339
                     /locus_tag="KPK87_002304"
     CDS             14428..15339
                     /locus_tag="KPK87_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG2538449.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15424..16128
                     /locus_tag="KPK87_002305"
     CDS             15424..16128
                     /locus_tag="KPK87_002305"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG2538450.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            16154..16711
                     /gene="frr"
                     /locus_tag="KPK87_002306"
     CDS             16154..16711
                     /gene="frr"
                     /locus_tag="KPK87_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG2538451.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16714..16917
                     /locus_tag="KPK87_002307"
     CDS             16714..16917
                     /locus_tag="KPK87_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538452.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16946..17680
                     /locus_tag="KPK87_002308"
     CDS             16946..17680
                     /locus_tag="KPK87_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG2538453.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17693..18472
                     /locus_tag="KPK87_002309"
     CDS             17693..18472
                     /locus_tag="KPK87_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG2538454.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7708 a         1859 c         3237 g         5913 t
ORIGIN      
        1 aataaatttg atttatatta tatagattta aagtaaaaat agttttattt aataattaat
       61 aaaaataaat acaaaacaaa ataaataaat aataaaatat ttagtttaat tatctttaca
      121 aataaaaatt ttagtaataa aataattgtt gaaaaagatt ttaaattcag attattcatt
      181 tctaaagtaa ttaatgtgca aatctttatt aatatatgtc aatagttagg tatatatgta
      241 tttaattagc atgatataga taaaagtatt tattttaatt gtctattatc tgaaaaagtc
      301 tttacttaga aatcataaaa atcaaattaa ttaggaaaag aaagggtgtg tataatgtga
      361 ttgagactgg atttgtatat tgtagtttct tggttgtatt cgtaggtgta atttttttta
      421 tggttgataa attgaaatgg aaggtatttg atgttattaa tccaatgctt ttaatatact
      481 taggttcagc tatacttgga tcatttggtt tatttgcaca gaatgaagat gttggaacgt
      541 atcaaaacat aataacagtg aacttccttc cattaatgtt agtgtttttg ttaattcaat
      601 gtgatttaag aaaaatatta aagatggggc ctaaaatgtt attatcattt ttctgtgcta
      661 catttacatt tatgcttgga tttgttatag cattcataat ttttaaaaat tcaatgggtg
      721 cagattcatg gaaaattctt ggggcagcat ctggttcatg gataggtggc tcatcaaata
      781 tgattgcagc agctaatgct ctaggagttg cagaagaagg acttagttat gcaatactta
      841 tgggttctat aggatataca gtatggatgt ctgtttgtct aatgtctgtg aaatttggac
      901 ctaaatttaa caaatggaca aagtgcgata caagttttat agatgaggtt acatcaaaac
      961 ttgaaaaaga aaataaagat aatacggcac ctacgtttgt agaacttatg attttacttt
     1021 ctgttgggtt tggagttgcg tcttttgcac aaattgtatc caagatactt ccaacttcag
     1081 gtatacttaa tgaaactatg tgggtagtac ttgttgcatc tacagtatca atattattgg
     1141 gtatgacaaa aatatcaaag cttaaaggga gtacactggt aggaaatgtg tttatgtatt
     1201 tactccttgc agctacaggt agtaaagcaa atttctttgg attgtcagaa gcacctgttt
     1261 acataatgtt tggattggta gtagtccttg tacatgctat ttcatttata gggcttgcta
     1321 aactatttaa acttgattta tttacttgtg aagtaggaag tatagctaat ataggaggag
     1381 taactacagc tccaatgatt gctggggttc acaatcctgt attaatacca gtaggtgtac
     1441 ttatggggct tctaggaaat attataggga cttattgtgc tcttatggta actcaaatat
     1501 tacatatgtt agcttaatga aaatatggta taaaattaat aatttaaata agaaaagtca
     1561 tttcaattta agcagaagct tttactgaaa tggctttttt tgtttgacaa gataatctat
     1621 ttcaagaaga tgttttgaca tatttagtgt ataatattgt aaaatttact aaaaacgaga
     1681 tatatttaat ttttatattg aatcgaacgt ctgtatgatg tataataaat atacagaacg
     1741 aatgtttgca gaacgggtgt tttttgaatt tattttaata atttatgaat atatatcaga
     1801 atgcataaat atatttaaat ataaagatta aaaagaattt aaaggagtgt tgataactta
     1861 tgataattcc atcaagaaga atacaagata taacattaac aaatttaaaa aatggtgaag
     1921 taactttaat tgaattggat gaaatataca agaagatggg atttttattt gttgttagtg
     1981 aaggcaaatt gaaaaagata aaaaaggaga atcatcatta gaaatgaatg aaataataaa
     2041 tattataata gtttataaaa cctaacttat gtagaatata aatacttaag ttaggttttt
     2101 ttgtgataaa gtttataagt actcaatttt ataagataaa taatggtctt aagtgaaaag
     2161 taaataagtt taaattttag taagtattat attgcataag aggtgatatt attatgaaaa
     2221 tagtaaccta caatattcat aaaggtatgg attcaaataa tagattaact ctaactaaaa
     2281 tggggttata tttaaaacaa ttggattgtg atgtaatttg tttgcaagaa gtattatatc
     2341 ctcaattttt agctttaaaa gctgttttaa atatggatgg tgtctttgct acaaatgtaa
     2401 aaaaagtaaa tatgatatat ggtatttgta catttacaac atctaaaatg ttaaacagta
     2461 atcatttttt cttaactagc aaaaaagaac aacggggagc tctttgtata actatagatg
     2521 catatggaag aattataaat gtaataaata ctcacttggg acttgatagg caggagagag
     2581 caaaacaatt agacgaaata atagactata ggaacagatt agtggggata gttattttat
     2641 gtggtgattt taatgagaaa aatgtctttt taagtatgtt taatgatatg gctatttcac
     2701 taaacaagtc ttacttacca acatttgaaa aatcaaactc tagaatagat tacatttttg
     2761 ttaataaaaa tactgaacta aaaggataca cagtagaaaa aatttacctg tcagaccatt
     2821 atcctgtaat aggttacatt taaatttcaa tataaatgaa attagttgtt ataaaataaa
     2881 caattttttg ttaagaaata gtaaaagaaa tataataaaa aatatcaaaa ataaaactaa
     2941 ttataagaca taaatttaca ataaagttac atttgattaa attccttgtt taacaaaagc
     3001 tattcaaata taatctaagg tagttttgaa atgggaactc tattatgcaa atttaagcta
     3061 gtgttaaaat gaggtgaagt tatgggcaga agaacaacaa gagtttcaag attaggtaaa
     3121 gaaaaaaaac gaagtgtttt aattgtattg gcagctttag ttctaataat gggtggattt
     3181 acttattttt ttaacagtaa atttctgtac aatgggaaaa tcgctaaaaa tgtatatata
     3241 gaaggagtaa atgtatctga tatgactaaa gcagaggcat taaaagctat aacagataag
     3301 tatactccag aagatttaaa tcttgcttat gatgggaaaa aatatacaat atcaccaaaa
     3361 gatattgatt taaaatatga tacagaagat gttgtaaaag atgcttatga aagtacaaaa
     3421 aaagggtcct attttcaaaa cttaaaaaaa tatattgata taagagtgaa taaggcaaat
     3481 atgaagataa aagctgaata tgatgaggca aaacttagtt ccaaagtgtc ttctatagca
     3541 gatagtataa atgttaagat gaaaaatgct tctattagtg ttggaagtgg tggactaagt
     3601 tatacagact ctgttgtagg tagagaattt gatttggcag caaataaaga gtctatatat
     3661 aatatgataa aaaataaaga gcataaaact ttagaactta aagtaaatct tcaaaaacca
     3721 gatataacaa ctgaacaagt aaaaactgta aattctgtta taggtcaata ttcaactact
     3781 tattcacaag ctgtagaggg taggtcatat aatgtaggat tatcagcaag aaaaactagt
     3841 gatgtattat taatgccagg tgaagaattt tcatacaata aacttacagg acctagtaat
     3901 aaagcaaatg gttacaaaga tgcacctgtt atagtttatg gtaagctaga acaatcagca
     3961 ggaggaggtg tttgtcaaac atcttcaaca gtttataatg cggctcttct ttcgggtatg
     4021 gagataactc aagttacaaa tcattcatct gcatctactt atgtgcctaa agggagagat
     4081 gctactgtga gcgatggagg attaaactta aagtttaaaa atccatataa gcatcctgta
     4141 tatattaaaa attatgcagg tggaggaagt gtatcatcag taatctatgg taattctggt
     4201 gacaaaccaa atatatctat agaggtaaaa caaactggtc agaataaata ttctacatat
     4261 cgaatattta aagattcaaa tggaaaggtc ataaaaaaag aacatatatc aaatagttct
     4321 tataaagaat taaaaaaata gataattttt tagagaaata tttgtaatat aaaagggagt
     4381 gtctcaaaat gaacttttta gtttagagac actcttttta tgtgaaatca aatatatttt
     4441 catcatttca acaatacaaa agaagcatat ctctattttg agacagcctt ttttgtaaaa
     4501 attaacacta ttagttcttg ttttaatatt acaatcatta atatatagaa aatattccta
     4561 tatattggta taattgtaaa tagatttagt tatataaaat gtatgaggag gtgacagggt
     4621 tgaattttat aggcaataga tatgaggtag taaattctga cgacgtttta gaaattaaca
     4681 aaatctataa agctagagat gttttttaca ggaaaaatgt gttaataaaa gttataaagc
     4741 acaataatta tatctgtgaa gattttgttt caaatttaat tgatgaaagt actgctttag
     4801 atgagataaa ctctccttat atactaaaaa taatagatgt tgggattcac tgtacagaag
     4861 aaacaacatt atattatgtt gtaagtgaag actttaatgg tatcggacta gatgaattaa
     4921 ttctaggtaa ttatcttcat ttagaagcaa ttgtaaacat aatgatacaa gttttaaaag
     4981 ctttagaaat gatacatagg aatcattcat atcatggtag tttaaaatcg agtagtataa
     5041 tagtagatac agaatacaat ataaaaattt gtgattttgg aattacaaaa gcaaataatg
     5101 gggttaatac aagaagctat ggaaacagga gatatttatg cccacatcaa ctatgtataa
     5161 attatactga taaagaaagt gatttttttg caacaggtct tattctattt gaatctatat
     5221 ttaaaaaatt gccttttgga gaatcaaatt cagaagaaaa aatgctacaa tctattgata
     5281 agggattaga ttggaatagt ataaaagcaa ttaatggaaa tactgaactt ataaatgtta
     5341 taaaaaggct tcttggaaga aataataagt ataccagagc caatgatatt attattgatt
     5401 taagtaaagt aatgtatgaa aaagcatata tagaagttga agatgagaaa gaagaagagc
     5461 agaaacaaga caaagttata caagttaaaa aagaatataa aagtaaaatg agaaaaaaat
     5521 tacataaaaa acttgttgtt gccggtctag ctatagtcat gatttcaatg attattatta
     5581 gttccataat ataaaaattg tatattaagt attttaaaat caatgtctat aaaggatata
     5641 catagaaatt ttagtgtagt cttaaattat gaaaggaagt ggttattatg ttaacaaaaa
     5701 ataatgaggt attaaacgtc taggagtatt ttaacaaata gccacctgct tttacagttt
     5761 agaataaaaa agctgtggct aaaggtcaca gctttttgct actttttaaa ggcaggagaa
     5821 tattaaaaag gagcaaatta tgagcaatat aaaaataata gcaagtaata aatgttggat
     5881 tgaagatata gcaaggaaac aacttgaaga tatttcaaag ttaaatggaa ttttaagagt
     5941 agtaggatta cctgacttac atgcaggtaa aatacctgta ggtttagcag ttgagactaa
     6001 aaatattatt tatccacata taattggaaa tgatatagga tgcggaatga ctttatttaa
     6061 aactggggtt ctaaaaaaga aatttaaaaa agacaaatgg attaaatcac ttagtaagat
     6121 taaagatttg tctgatatag aaataaaaaa cacatataaa gaagaatgtc caattttaaa
     6181 tttaggaact atagggagtg gaaatcattt tgtagaaatt caatgtataa gtgaaattta
     6241 taataaagaa caatttgaac aattaaaatt ttcatctgat gatgttatga tgttagttca
     6301 ctgtgggtca agaaattatg gagaagatat tttaaaaaaa ttttatgata aagatggact
     6361 tgaagttgaa agtgagaaag ctatagatta tattaaaaaa catgataatg ctttaatgtg
     6421 ggcagagcgt aatcgtgaag ctatcagtaa aaaaattatg cagtcaatag gtacatctgg
     6481 agatgttgaa acaatattct ctataaatca taattttata gagaaaaggg aagataaatt
     6541 tatacataga aaaggtgctg tttcaagtga aagaggagct gtgattatac caggttcaag
     6601 aggcagtcta tcatatatag ttatgcctac tgaaaataca gagatatctt tatattctct
     6661 atcacatgga gcaggtagga aatggtctcg ttcagtttgt aaatctcgcc ttaaaggtaa
     6721 gtatagtaaa gatactataa aacaaactaa atttaaaagt cagattatat gtaatgatgt
     6781 aaatttatta tttcaagaag ctccagaggc atataagaat atagagcaga ttattgaaag
     6841 tttgatagaa tatgaactta ttcaagtagt ggcaactatg aagccattga ttacttataa
     6901 aggataattt ttaaagttaa aaaagattaa aattatatta taaatagcta attttataga
     6961 acatttaaaa gtgttttatt attagctatt tttttatttt gtagagacga taatttatag
     7021 agctaaattc tttgatatat aatgattaca aagttagact gtattggaaa atgcacatac
     7081 atgaattgat gacaatataa gtgattccca aatataaatg aaagtataag cttattttca
     7141 ctataaaaat tttcactgta caaaaacatt aatgacatta tttttattga cataaatata
     7201 taatttatgg aataatatta gaagcactga tataagtgtt taaataagaa tagaaaagga
     7261 gaagtgtttt atgaaaaaaa atactaacat taatgagtcc ggagttaaac ttcttaataa
     7321 ataaggagtt tgatatataa taactcctta tttattaagt tgttcgcaaa ataactatta
     7381 tcaaagtaaa tgatagggtg atttaaaaga cgatgactta taaataaagg agaaaaaata
     7441 atgaatagta atacatttga aagattacaa ctaaatgaag ttaaagactt aataaaaata
     7501 cattgtgtaa gttcgctagg aaaaaattta atagataaat taactcctag tgggaatatt
     7561 ggagtagtaa gaaggaaatt agcagaaaat aaagaagcta gaaagataat tgaaaatagt
     7621 aatcacatac cacttgaagg attgtttaat gtaggttcta caatagataa aattgaaaaa
     7681 ggaatgataa tagaacctgt tgaacttgta aatattgaag attttttaag aggttgtagg
     7741 aagatgaaag cttttatgtt agaaaaagaa ttttattcac ctacattaag ttcttatgcc
     7801 ttaaatataa cagaatgtaa aagtatagaa gatgagataa attactgtat aaaatctaat
     7861 aaagttgatt ctaatgctag taaagaatta aaaaagataa gacgaaatat agaaattaca
     7921 gaaggaaaga taaaagatag attaaataaa tttataacat caactgtaaa taaaaagtat
     7981 atacaagagt ttataataag taaaagaaat gatagatatg taataccaat taaatcttct
     8041 tataaaaatg aagttaatgg tactatatta gatacatcat caaaaggaaa tacggtattt
     8101 attgaaccta ttagtgtttc aaatttaagt acagagttaa ctatgttaaa agctgatgaa
     8161 actattgaag aatacaaaat attatcttat ttaacagaac ttatatttga gaagataagt
     8221 cagataaaat taaatataga gatactttca gaatacgata tggtatttgc aaaagctaag
     8281 tatagtcaaa aaataaaagg aattactcca aaaataaata ataatggcta tataaaaata
     8341 attaaaggta aacatccact tctaacagga gatgcagtac ctttagattt tgaaataggc
     8401 aaaaattata gaagtcttat aataacaggt cctaatgcag gaggaaaaac tgtaaccctt
     8461 aagactgttg gtttactaac tttaatggtt caatgtggat ttgatatttc tgcaaaagaa
     8521 ggtagtgaat ttagtgtatt tgaaaaagtt tttgtagata taggggataa tcaaagtatt
     8581 gaaaatgcct taagtacttt ttcatcacat ataaaaaata ttgcagagat tatgagttta
     8641 tcaaataatt ctactcttgt tctgtttgat gaaataggaa gtggaactga acctaatgaa
     8701 ggagctggtt tagcaatatc attattagaa gagttttaca aaatgggctg tataactata
     8761 gcatcaactc attatggaga gataaaaaag tttgctacat tgcatccaga atttgaaaat
     8821 gctggtatga tgtttgataa agaaacgtta gaaccattgt ataagttaac tattggaaaa
     8881 tctgaagata gtaatgctct ttttatttct aaaaaaatgg gaataaaaaa tagagtttta
     8941 gatagagcta aagaatatgt aattgataga aactacaatt tagatttagt taaaagaagt
     9001 aagctacaat ctaatattga agataaaaat agttctgaag tcattttaaa ctatacagat
     9061 tataatgtag gggataaggt aaaattacta gatgaagatg attttggaat tgtatataag
     9121 cctatggaca agtttaataa tgtagaagta ttgtttaatg ataattttat ttcagtaaat
     9181 ataaaaagat tacaacttag cataaaagca tcagaattgt atccagaagg gtatgattta
     9241 gatgtattat ttactagttt taaagaaaga aaattagaaa aagatattca aagaggttca
     9301 aaaaaagctc ttaaaaagat tcagaaagaa attagagaga atagaaaata aatataaaaa
     9361 gcacaaaaga aagcaatcta aagagaaaag tttatcttta gattgctttc ttattaacat
     9421 atcttcataa atccaatgac taacaagatt aaaccactta accaagataa gtttaaattt
     9481 acgtttttaa caaacatatt tcctagtaaa caaccaagta atactgcaat tatatttgat
     9541 attaaagaaa atagaataac ttgtatataa ttcacaccta caaggccact accaaaaccg
     9601 atagctaatc catctaaact taaagcaagt gcaagtgtaa atgcttcagc agatttaagc
     9661 actttagatt tacttgtatc taagacatca ggttcgatac ttacattaaa atttaattta
     9721 aggtctaaca tattaaaatt ataattcttt gagtgtatag attttttctt aaaataagat
     9781 ttaaaaaaac tttcaattag tctagcactt cctaataaaa ataaaataaa gaaacatatt
     9841 aacattgtta tattttgagg taaaaagtct ctaactattg tacctgcaaa aagcgatatt
     9901 gctaaaatag ctgaacaaac tacgctgata attatatttg aatataaggg aactttaact
     9961 ttatttacgc catatccaaa gcttgtcaca aaggcatcta tacaaagaga agtaactaat
    10021 aaaattgatt caatcacagt agattaacat cctttctctt aaattttgta cttatattag
    10081 ttatagtatt gtaaaaatgg ataaattgtt acagataatc caataaattg ttgataaaat
    10141 tccaacatca tacaacacct aaataacaaa agaatcatat catataataa tataaaacca
    10201 atcataataa agcgggtgaa aataatggaa caaaataata aggttgggaa tttcggtgat
    10261 ttgttgaaga ttttattaga tgaaaaagaa ttatcaatgg gagaactaag taaaaaaagt
    10321 ggaattgata aatctactat atctcgtata gctaataata aacaaaagcc aaatataaat
    10381 catttagaaa agatggcaat acatttaaat attaacctag aagaactgtt gaaagcttca
    10441 ggatatgaat ttagaaacag caataatcaa atatttaatg cagatagtga ttttagtaac
    10501 tttgatgata ttcttggatt tgcaaattta atcaatgata agaactttaa tggaaatatt
    10561 gaaaaagagt tgagtaagtg taagttatat gtccaaacag atgaaggtaa aaaattactt
    10621 tttgataatt ttaataaaaa aatagattct attgaaaaac aaggtcaatt tacagataga
    10681 ttaagacaaa tgtatgctga tttttgtaca gatggactta caataaagaa atatttattg
    10741 attggaagta tgctacttta ctttgttata tcaacagatg tgattccaga cttcgtgttc
    10801 ccaataggat ttatggatga tttagtagca ttaaatatag ttacaaaatt acttaaaaat
    10861 gataagtaag ttttagaatc atatttaata tataaataaa tcaaagtata taaaatttat
    10921 tattttttat atgaaacaaa ttaaaagcac aatgaagtag attaaaaatt taaaactaag
    10981 aatctaatat atattttgta actatttaag tatataaaat aaaataatat ccaaactaat
    11041 acaaaatagt aataattgtt ttgtactgga ggatatatat ttatgaattg taaatggata
    11101 tctaaaatag atgagagaaa aaaatgtcat agagaagcag attcttctgg atattgtatc
    11161 tttcataaag aaaataagtc tgatgaagag atacaattga tgatggatac tcttcataaa
    11221 gaagagataa gtgaatttaa tggatttgtt tttgaaaatg aatttaatgc ggaagaaata
    11281 ttaacttaca attataaaat actggatttt tctgaatcca tatttaaaca aaaagcaaat
    11341 tttaaaaaat atatttttaa aaagaatatt atttttaact atacagaatt tagagataaa
    11401 gttttattta atggttgtgt attcttagaa aactgtgatt ttaatagaac catatttagt
    11461 aaacactata taaatgaccg tatatttgaa aaagtgaaat ttaaaggacc tgatttggta
    11521 gttaataaag tagaaaattt tccacgaatg gatggaataa tttttagcat gtgtacaaaa
    11581 tttgtgttaa agaatgttga atatggaaaa agtgagtatg agcatggcaa gataaattat
    11641 agaatagcta gaaatcaagc aacaaaaatt ggagaatatg agatgatagg attttactat
    11701 tataaggaaa gaatttatag tagtaaaata atgaaacgtt ctaattatcc tactttcagt
    11761 gattatttag ttgaaaaatt ttttgaccaa atagcacgtt acacaacagg ttatggagaa
    11821 aagccatgga atatattatt agttattata gcaataataa gtgtttttgc tttattatac
    11881 ttatttgtag gcatagaaag ttcaaatagc acacttgtag ctttagatat aaacaatata
    11941 ggtgattact cactaagtga gattttcaaa atgtatatgg atttatggta ttttagcatg
    12001 gctacattta gtacagtagg atatggagat atggttgcta caagtttgat tgggaaagca
    12061 ttggcaggta tagaagtatt ttttggagtt acaataggag caatttgggc atctgtgatt
    12121 attaaaagaa tgataaggta atatttgtgt aaatatggat aggtattaat tactagatat
    12181 aaattggact aaatagggaa aatttaaata taaatatact taataatcca agaaggtgaa
    12241 aaatttgaaa aatgcactaa aaaaaattat atcattagcc atgattctag tttttataat
    12301 accaaattat tcatatgcag atgataaatc tgtagacaag tattcaaaaa catctatact
    12361 tatagaccaa gaaacaggta gagttttata tagtaaagaa cctgatatga aggtaccatt
    12421 agctagttta agtaagatga tgactttttt acttgcaata gaagctattg aaaataaaga
    12481 agtaaaagaa actgataaag taaaaataga caaatctatt gcatcagtaa aagggtcaag
    12541 ttataaactt aaagatggtg aagaagtgcc acttatagag cttatgagag gtcttatgat
    12601 tgtatcagga aatgatgcgg caattgctat agcaaaacat atttgtaaaa cgaaagaagc
    12661 atttgtagat agaatgaata aaaaagcaaa agaaatagga atgagtaata cacattttct
    12721 aaatccaaat ggtcttccaa tatacgattt aagcaacccc aaaaaaccag caaaagaaaa
    12781 tatctctact gcaaaagata tagctattct tggaaaatac atgtttgacc attatgaaaa
    12841 acaagtaaca gctataactg atatggagac atatacaaat tcagatagaa gttttgaaaa
    12901 aagcaataca aatgcattat taagaattat accagaagta gatggtataa aaactggata
    12961 tactggcaat gctggatatt gcttatcatt ttctatggtt gtaaataaaa atgataaaaa
    13021 tgagaaaaaa cgtagagtaa taggggttgt attaggtact aaccataaaa ataaaagaac
    13081 ctctgcttca ttagctctac tgaaatatgg aaaagaaaat ttcaatatga aaaaagtaat
    13141 tgataaagat agctttattg gtaagaaata tataaaagga atgaaagagt tagaggttac
    13201 attaaaagca aaagatgaac tttatacaat attaaaagat gatgaaagta taaaatcaga
    13261 agttaagatt aaaaatatag agtaccctgt taaaaaaggt gatactatgg gtattataaa
    13321 atattatact aattcaggtg acttacttgg aagtgtagat atagtaagta ataatagtat
    13381 agataacgta tcattaaaaa caaaattaaa aatgaaattt gcaaattaaa ttcaaaaaat
    13441 ggtataaaat acacaaaaaa atataaaaat agaaaatatg gaaaaaatat tgttatttct
    13501 tcacctctgt gatatactac tagaggtgaa aaaataaaat aaatcacaca cagcattggg
    13561 atccttaaaa ggtgcctatt acatggtttt ttagggagaa gatcaagctg gaggtataaa
    13621 accaggaggt aagatatgtc agtaatatca atgaaacaat tattagaagc aggtgttcac
    13681 tttggtcacc aaacaagaag atggaaccct aaaatggcta agtatatatt cacagagaga
    13741 aatggaatat atataatcga cttacaaaaa acagttaaaa aagttgaaga agcttacaag
    13801 ttcactaaag aagtagctga aacaggaaag ccaatcttat tcgtaggaac taaaaaacaa
    13861 gctcaagatg cgataaaaga tgaagctgaa agatgtggaa tgtactttgt taatgaaaga
    13921 tggttaggtg gaatgttaac aaaccacaaa actataaaaa caagaataaa taaattaaga
    13981 gaattagaaa aaatggaaga agaaggcgta tttaatgttc ttcctaaaaa agaagttata
    14041 aaattaagag ctgaaaaaga aaaactagaa aaatacttag gtggaataaa agacatgcct
    14101 gaattaccag gtgcaatgtt cgtagttgac ccaagaaaag aaaacatagc tattcaagaa
    14161 gctcatagat taggtatacc agtagttggt atagttgata ctaactgtga cccagaacaa
    14221 ttagactttg ctataccagg aaatgatgac gctataagag ccgtaaaatt aataactggt
    14281 gctatggcaa cagctgtaat tgaaggtaga caaggtgctg aggaagaagt agctgaagac
    14341 caagaataat aatttgacaa ttaaatggta agggtcgccc ttaccattta taatatggaa
    14401 tagaataagt aggaggaaac ttaaaaaatg gctaatataa ctgctcaaat ggtaaaagag
    14461 ttaagagaaa gtacaggcgc aggaatgatg gactgcaaga aagctttaca agaagctgaa
    14521 ggaaacatgg aaaaagcagt agatttatta agagaaaaag gattatcaaa agctgctaaa
    14581 aaagctggta gagttgctgc tgaaggtcta gttgcaatag aaatgaatga tgataataca
    14641 gttgcttcta tggtagaagt taactcagag acagatttcg ttgctaaaaa cgaagacttc
    14701 aaagtgttcg ttaaagatgc tgcttgtatg gcattagcta ctgataaaga agatatagct
    14761 tctttattag gtgaaactca taaagaggga ataactttac aagaagtttt aaataataga
    14821 gttgcaaaaa taggtgagaa attagacttt agaagatttg ctaaagttgt aactaatgga
    14881 caagttgctg gatacattca cggtggtgga aaaataggtg ttcttgttga aatggaaaca
    14941 gaagctagag atgctaaagt tttagaatta ggtaaagatg tagctatgca agttgctgct
    15001 atgaatccta agtatgtttc aagagatgaa gtagatgctg aatacatagc acatgaaaca
    15061 gaagtattaa ctcaacaagc tttaaatgaa ggaaaaccag ctaatatagt tgaaaaaatg
    15121 gttaaaggaa gattagaaaa agaattaaaa gaggtttgtt tattagagca aactttcgtt
    15181 aaaaatccag atataactgt taaacaatta gttgctgatg ttgctaaggc agtaggttct
    15241 gatataaaag ttgttaaagt tgtgagattc gaagttggtg aaggtataca aaagagagaa
    15301 gaaaactttg cagaagaagt tgctaagcaa cttaagtaat aaccaaagag agaacacgca
    15361 ggtgttctct tttttaaaaa atagaaaaaa ttggatatga aggattgaaa taggaggttt
    15421 ctgatggata aaccaatgta caaaagggta ttgttaaaat taagtggaga agcattagca
    15481 ggagaaagag gttttggtat aaataatgat gtagttaatg atattgctat tgctataaaa
    15541 aaaatacaag aaataggtgt tgaagtagct gtagtagtag gtggaggaaa cttctggaga
    15601 ggcagaacca gtgaaggaat ggacagaact actgctgact atataggaat gttagctaca
    15661 gttatgaatg caatggcgct acaggatgca ctagaaaata tagatgttgc tacaagagtt
    15721 caaactgcta tagacatgag acaaattgca gaaccatata taagaagaag agcagttaga
    15781 cacttagaaa aggaaagagt tgttatattt ggtgcaggaa caggaaaccc ttactttaca
    15841 acagatacaa ctgctgcttt acgtgctgct gagatggaag cagaagttat attgttagct
    15901 aaaaatgttg atgcagtata tgacaaggat ccaaaagtac atgcagatgc taagaaattt
    15961 acagaattaa gttacatgga agttatacag aaagaattaa aagtaatgga ttctacagct
    16021 acatctttat gtatggataa taaaattcca ataaaggtat ttgagcttac aacagagaat
    16081 ataataagag cagtaaaagg cgaaaatata ggaactactg taaaataatg aagaatgaag
    16141 gaggagtaaa attatgaaac tggaaataca taagcaatta gaagagaaaa tgaatgggac
    16201 aattgatgct ttaaagtttg aatttggaac tataagagca ggaagagcta atgcacaaat
    16261 gctagacaaa ataagagttg attattatgg aactccaact ccaataaatc aaataggtgc
    16321 tatatctgta ccagaaccaa gaatattaat gatatctcct tgggataaat ctgctatgca
    16381 tgaaatagaa aaagcaatag ctaactcaga cttaggatta aatccatcaa atgatggtga
    16441 ggttataaga ctttctgtac ctgctttaac agaagaaaga agaaaagaat tagctaaaaa
    16501 agctagtaaa gctgcagaag aatttaaagt tagaataaga aatgaaagaa gagacgccaa
    16561 tgagaagatt aaaaagatgg aaaaaggcgg agaattaact gaagatgaat taaaaaaagc
    16621 acaagatgaa gttcaaaaaa tgactgataa atttataaaa gaaatagata cattattatc
    16681 taaaaaagaa aaggatataa tggaggtata atattgaaaa aatattataa ccttttaggt
    16741 cttcatgtag atgaggtaaa acaatatttt gatgagcaaa atataaatta taccgtaaaa
    16801 tctattgaag gtaaaaagga taaagaaaaa cttgtagttc ctagagttat aaaaatatct
    16861 gagataggtg atagtgtaga actgataatc acatactttt ctgactcctt agtttaagga
    16921 gtcagaacaa tattggaggc aaaatatgaa taacaacatt atatatgata tagacttaaa
    16981 taacatacct actcatattg ctattataat ggatggaaat ggaagatggg caaaagctag
    17041 atttcttcca agaactgcag gacataaggc tggagtagaa actataagag atatagtaaa
    17101 agaatgctca aaattaggtg ttaagcactt aacattatat gcattttcaa cagaaaactg
    17161 gaaaagaccg aaacttgaag ttgacacact tatgaactta ttatctacat acttaagaaa
    17221 tgaaatagct gaacttcacc aaaacaatgt aaaagtaact gctataggag atataagtgc
    17281 tttaccaaaa acttgtataa gagaattaaa ctcagctaag gaactgacaa aaaacaatac
    17341 aggagttaac ttaaatttag cattaaacta cggaagtaga gcagatataa aaaatgctct
    17401 tatagatata gtaaaaaatt gtgaaagtgg taaaatagat ataaacaata ttgatgaaga
    17461 tattataaaa aactatttaa gcacaaagtc tataccagat ccagacttgg taataagaac
    17521 aagtggtgaa caaagattga gtaacttttt actttgggaa gttgcttatt ctgagtttta
    17581 ctttactgat atacattggc ctgattttaa aaaagaagaa ctacagaagg ctatatacgt
    17641 atatcaaaga agggatagaa gatttggagg acttaagtaa ggagattatt ttatgcttac
    17701 aagaattatt gcttcactag ctctggttcc attatttcta tttgttgtat atggaggtat
    17761 tcctctatat atagctgaga cagcaatagt ttatattgca ttgcatgagt tttataaggc
    17821 atttaaaata aaagatgtac atccaatatt cattatagga tatttatttt ctatttattt
    17881 agctgtaaaa aatattttta atttaccatt agaatatact tatgcagtaa tttttatttt
    17941 atttttagca agtataattt atatgttgat gggcaaaaac aatgttatag atgtttcgat
    18001 tacgttttta ggagtttttt atataggagt atttttagat tttataatta taactataaa
    18061 tggatttgaa aaaggtagta tatatgtatg gcttatattt gtgatatctt ttatgacaga
    18121 tatatttgca tattttagtg gttatttact tggaaaacat aagctaattc ctaaagttag
    18181 tcctaaaaag actatagaag gagctattgg aggaataata ggaagtacac tttgttgtat
    18241 tttatttgga tatttatttg gcattgattt gcttcaatta gcgataatag gaagtatagg
    18301 aagtgtaata gctcaattag gagatttatt tgcatcttct ataaagagat atgtagggat
    18361 aaaggattat ggaaagataa tacctggtca tgggggtata ttagatagat ttgatagtgt
    18421 tatattagtt gcaccttttg tatatagtgc gattaaattt tttattagat aaagattggt
    18481 tggatttaaa agagcaatct ataattgcta gtaatgaaaa tagctatgta acttttaaat
    18541 gcagagatat tctgcagtta ttagttacat ggctattttc attgttcaaa aatttagaag
    18601 accttgggga attgtataag aataggtatt actttataat agtatactat atttgtagtg
    18661 agtatatttt ttatactgta actatcatta aaaaatattt ttttgtttaa taatttc
//
LOCUS       DAEMIK010000082        18490 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEMIK010000082 DAEMIK010000000
VERSION     DAEMIK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.082"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            426..1469
                     /locus_tag="KPK87_002310"
     CDS             426..1469
                     /locus_tag="KPK87_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538455.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1580..2722
                     /locus_tag="KPK87_002311"
     CDS             1580..2722
                     /locus_tag="KPK87_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2538456.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKAKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            3176..4567
                     /locus_tag="KPK87_002312"
     CDS             3176..4567
                     /locus_tag="KPK87_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2538457.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            4820..6613
                     /locus_tag="KPK87_002313"
     CDS             4820..6613
                     /locus_tag="KPK87_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2538458.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(6694..8076)
                     /locus_tag="KPK87_002314"
     CDS             complement(6694..8076)
                     /locus_tag="KPK87_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2538459.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGFSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            8334..8681
                     /locus_tag="KPK87_002315"
     CDS             8334..8681
                     /locus_tag="KPK87_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2538460.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            8699..9733
                     /locus_tag="KPK87_002316"
     CDS             8699..9733
                     /locus_tag="KPK87_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538461.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(9782..10831)
                     /locus_tag="KPK87_002317"
     CDS             complement(9782..10831)
                     /locus_tag="KPK87_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2538462.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            11106..12641
                     /locus_tag="KPK87_002318"
     CDS             11106..12641
                     /locus_tag="KPK87_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538463.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVTIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(12826..13512)
                     /locus_tag="KPK87_002319"
     CDS             complement(12826..13512)
                     /locus_tag="KPK87_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG2538464.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            13637..15019
                     /locus_tag="KPK87_002320"
     CDS             13637..15019
                     /locus_tag="KPK87_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2538465.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            15421..16689
                     /gene="eam"
                     /locus_tag="KPK87_002321"
     CDS             15421..16689
                     /gene="eam"
                     /locus_tag="KPK87_002321"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG2538466.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(16782..17138)
                     /locus_tag="KPK87_002322"
     CDS             complement(16782..17138)
                     /locus_tag="KPK87_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538467.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(17289..18383)
                     /locus_tag="KPK87_002323"
     CDS             complement(17289..18383)
                     /locus_tag="KPK87_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2538468.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         7359 a         2152 c         3009 g         5970 t
ORIGIN      
        1 tattattgaa tatattttta catataagat ataaggtgat agatagaaat tgttggatta
       61 tgtaaataat taatatataa taccattatg ggcaataaat acaaatcaaa tgataatata
      121 gtctatttat gtaaatgtta tattgtatcc taagtataat agtaagtttt ttgatagtgg
      181 tgtagaaagt aaaaatctac tagcctaaat tgatgtatgg attagtttag gaacagtaat
      241 tgcatatctt aagtttgatg tcatgttggg ctactagaaa taggtcatct ttttcacttg
      301 aatatctttt ctttaaacat ggggagtatc atatcaaaaa catatgaaaa ttaagtgatt
      361 tataagaaca gtaatatttt atatgatagt atagaatagt aataaagtat taggaggaac
      421 attgagtgtt aaaaaagtgg tttggtattg tgaaaaaaag acaaaaaagt gagtcagtaa
      481 aagaagaagg tgaagtaata ttaaaaaacg aaaaaatatt atctgaagaa aagttgatag
      541 atgaagaagg agttgtagtt aatattgata atgaaatatt aactaaagta gaagtagtaa
      601 atgatgataa tgaaatagaa gaaaaaataa tagaagaaga ttggttaatt agtgaaaata
      661 ccataaaatt agatgataaa gaagcaatta ttaatgataa aaacatagaa ttatgtaaaa
      721 aagaagttca agttgaaagt gaaaaaatag gtttaaataa gtttgaagga cttgaccaaa
      781 atgaaaatta taatctagaa aaaaatgtta ttgaagaaaa aaaagtaagt gaatgtttga
      841 cagaagaaga tttagagtac ataaaagaaa ttaaaataaa aagaggaaaa agtataaaag
      901 ctataaactt gtatactaaa gaagagtggg tttttgacac tcatatacag tgtagtaaaa
      961 aactcaaagt tccattaggg tacataagag aaaatttaaa atatggatat atggattact
     1021 ttggagatgc aataaattat ttaagtgaag tattaaatat agatgaatac tgcaaaagtg
     1081 aatggagcta tctcgataat agcaaatctc catctgagat atttaatatt ctaaacaata
     1141 aaatatttag tataaggctt tctaatgaaa aaagaaatga aattttgaca aatgataaaa
     1201 ttgaagcatt aaaaatgaat tatagatttg aatgtattga tgaagaatat gatgaatatt
     1261 ttaaaaaata taagtctata atcaaaagag gtggaaagaa aaaagttgaa ttagtaaata
     1321 aaaaaggtga cattttagaa atatttaagt ctttagaaga atgtgccatt tatttgcaaa
     1381 aggaaaagaa tgaagttata caaatgttaa aatatgggga tacaaaagta ggaagaaact
     1441 ttataaggta tagtttgaga agtatttaat ttcattttaa attcataaga gtttcatttc
     1501 tgattcacac atttaatatt attttaatgt aaaataataa aggtgaagta ttgcacaatt
     1561 aaaattaagg agggtattaa tgtctgaaga aaaaaatcca aaagaaaata cattagatga
     1621 atctaaaaat tcagataatg taaattctaa aaaagatgat gtgtcagatg aaaaaaatac
     1681 tgataacaaa gttaaaaaaa gggaaataaa gctaaacaac aaaacactta aagctattgt
     1741 agcaattgtt gcagtaggag ctatatgttt aggtggaggc tatgtttttg gcaaagaagc
     1801 tggaagaaaa ttgcctgcta cttcaaaaca ctataatagc agtgaggtag tagctactat
     1861 tggggataaa aaaataacag gtgaaatgtt aagaaagaga atggagccat tattttattt
     1921 aaatgcaaaa actactatga ctgatgacga aatacgttca tatgaatcaa atatgattga
     1981 aactatgatt acatctgata tattatcctc tgaagcagca aaagagaaaa taagtataac
     2041 taatgaccaa ctagatgctc agtataataa tttaatgagt agtatagaat cagcaatggg
     2101 aatgactaaa gaagagtttt taaaacagtt tgatttgact gaaaaatata taaaagatga
     2161 tatgaaaaaa gagctagtag catcaaaata tttaaatgaa aaagcaaaag ttactgataa
     2221 agaagcagag aactattaca ataaaaacaa gagcaattat ttacaagtta gagcttcaca
     2281 tattttaata aaaactgttg atgataaagg taagcaagtt agtagtagca aaaaagctga
     2341 acttaagaaa gaagctgaag aaatacttaa aaaagcccaa gctggggaag attttgctac
     2401 tctagctaaa aaatactctg aagatagtag tgcagaatca ggtggagatt taggtttctt
     2461 tggtaaaggt cagatggtgg aatcttttga aaaagcagcc tttgcattga agaagggaga
     2521 ggtatctaac aaacttgtag aaagtgatta tggatatcat ataattaaga aaactgatga
     2581 aaaatatcag ccatttgaag aggtaaaatc agatttagta agcagtttaa catcagaaaa
     2641 acaaaaccta cttatacaaa atctaaaaga aaaatataat gttaaaatta cagataaata
     2701 tcaaatgaaa gctaaagaat agataaagtc aatttattaa aaaaaagaaa aattaactgt
     2761 atggataaaa gctattttta ctctcaatat agagagtata aaatagcttt tattattttg
     2821 caataaaatc tatttatata tatgaagtga caaagaaata acatgcaaaa agacattaaa
     2881 aagaaatatt tttaatttga atttttttgc aaaaaattta caaaaatgaa aaaataaaca
     2941 aaaaagaatt gaagatttaa gttgttattt aaatatatat atattaagat tttcttctta
     3001 agatactaaa aaaataatat ttttcataat tttaaaaata ataataaaaa aatgaaaata
     3061 caggcgataa tctattgagt aaacatagaa aagatgatat tataagtatg tgctaaaatg
     3121 gttaaagcaa tattaatatt aaatttaact ataaaacaaa agggggcgtt tttttatgtc
     3181 aagtacagaa agcgtatcta aaaaaagtgg tcatccgaag agtttttggc ttatttgcat
     3241 gacaattttg tgggaaagat ttagttatta tggtttaaca gctttattgg tattattttt
     3301 tacagcaagt gtagctcaag gtggtttagg tctttctact caagaagcaa catccatgtt
     3361 tggcttattt acaggtctta tatatcttac accattagtc ggtggctggt tagcagataa
     3421 agttttaggt caacaaaaat gtatttctat aggtgcactt ttaatagcaa taggagattt
     3481 cgtactattt gcttcatcta catctattgc aatggtttgg gtaggtctat caataattat
     3541 tttaggaaat ggttttttta aatcaagttg ctcaaatata gttggggata tatatcctaa
     3601 aactgatgtg tcaagaaaag atgcagcata cagtttattc tatatggcag taaatgtagg
     3661 agcatttata gctccaataa tatgtggatt gctttctgat aatgtatttg cagtaagagg
     3721 agcaagtgga gatatagtat cttatggata taagatggca tttttaattt gtggtatagg
     3781 catgatgtta ggtatgatta tatttacact tcttgcacca aaattattaa atgaagtcgg
     3841 aaaatatcct gtgactaaag gtaataaagg tgaaaaaatt gagtatggac cacttactaa
     3901 gttggagaaa aataggataa tagcaatggt tattttattt ttatttgtgg tcttattctg
     3961 gacagcatac tatcaaacac aaagttcttt tatgatttat gtaagggata atgtaaatag
     4021 aactctattt ggatttgaaa tgccagtagc atggcttaca tcactaaatg ctatattatg
     4081 tgtggcttta tgcccagtat tagctaccct atgggttaaa ttatctcata ccaaaagagg
     4141 tgatttctct attccagtta aaatgggatt aggaatgata ataatgggtg ttgggtttat
     4201 agttatggta tttgctacat tagctaatgg tccagatgga atggtaaagg ctagtatatt
     4261 gtttatagta ttgacttata tatttacaac agtaggtgaa ttattcttat ctcctatagg
     4321 acttgcaatg ttcaataaat tagctccagc taagtatgct acgttgtcta tgggagcctg
     4381 gtatctaaca agtttttttg caagtttatt atcaggaaag attgctggtt ttacagccac
     4441 acttggtttt ttacagatat ttggaggaat agcaacagtt ttaataatat ttggtttaat
     4501 attattggga ataagaaatg tattaacaag attaatggct atggatttac taacaaaaga
     4561 aaaataattc atgacgaatt attgtaaata aatgaattac tttaaataaa ggaagttaca
     4621 tttaaatata aaattaaatg taatttcctc tttgattatg aaaaaaagct ttacacaaat
     4681 agatattata attaaaatta taattgtgat aattagagtg tataagattt atataaatta
     4741 tacaaaaaat atttatgtaa taaactttac atacttatat atttagttat tcaaactatt
     4801 atttcgagga ggtcttgttg tgaatataaa agatagatta agtggattaa gaaaatttat
     4861 ggaagaaaaa aatatagatg cctatatgat tccatcttct gataatcacc aaagtgaata
     4921 tgtaggagat tacttcaaat caagagaatt tatatcaggg tttaatggtt cagcagggac
     4981 ggttattgtg actaaagacg aagctgggtt atggacagat ggaagatatt ttatacaggc
     5041 agaatctcaa ttagaaggaa gtacaataaa attattcaaa atgggacaag aaggttgccc
     5101 aactacagat gaatatttat ataaaaatat tcctgaagga ggaactttag gatttgatgg
     5161 tagagttata tctgctagag aaggagctac attagctgaa aaattatcta aaaaaggtat
     5221 aaagatagaa tatcagtatg atttaataga tagtatatgg ccagatagac ctgctttatc
     5281 agatagtaaa gcatttttat tagatgttaa atattgtgga gaaagtttta gctcaaaatt
     5341 agctagatta agagaaaaaa tgagtgaaaa aggtacttct actcatgtaa taactactct
     5401 tgatgatata gcatggttat tcaatataag aggtggagat gttaaatata atccagttgt
     5461 actttcttat gcagtaataa ctttaaaaga agtttatcta tttgttgatg aaagtaagtt
     5521 aaatgaagaa atactaaatg aactagctaa ggaaaatgtt caaataaagc catataatga
     5581 tgtgtatgaa tttgtaaaaa atatagacaa aactgaaaaa gttttattag atggaactaa
     5641 gcttagttat acaatttaca ataatatacc ttgtgaagtt gagaaagttg atgagttcaa
     5701 tccagtaatg ttttttaaag ctcaaaaaaa tgaggtagaa ttagaaaata taagaaattc
     5761 acatgtaaaa gatggtgtag ctttcactaa atttatgtac tggttaaaga aaaatgtagg
     5821 taagatggaa atcactgaaa taagtgctac tcaaaaatta gaggacttaa gaagagagca
     5881 agaaggtttc tttgagccaa gtttcaatac aattgctgca tataaagagc atgctgcaat
     5941 gatgcactat agtgcaactc cagagtcaaa ttataaatta gaggcagaag gattattttt
     6001 agtagattca ggtggacaat attatgatgg aactactgat ataactagga ctacagtttt
     6061 aggacctata agtgatgaat taaaactaca ctttacttct gtagcaagag gaatgataaa
     6121 tctttctaaa gctaagttct tgcatggttg tagaggatat aacttagaca tactatctag
     6181 aagttgcatg tggaatatgg gaattgatta tcaatgtgga actggacatg gtataggatt
     6241 tgtgctaaat gttcacgaag ctccaaatgg atttagatgg agagtagtac ctgaaagatt
     6301 tgatagtgca gttcttgaag aaggaatggt aactactaat gaaccaggta tatatataga
     6361 aggttctcat ggtataagaa cagagaatga aattgtagtt agaaaagctg aaaagaattt
     6421 ctatggtcaa tttatggaat ttgaagttgt tacgttagct cctattgact tagatggtat
     6481 agtaccagaa ttaatgaata aggatgaaaa agattattta aactggtatc ataagttagt
     6541 ttatgataaa atatcacctt tcttaactga tgaagaaaga gaatggttaa aagtttacac
     6601 tagagctata tagtttgtac tagatacaat aaaaatcgag gtattataat gcctcgattt
     6661 tttattgtat attttattaa ctttaaaaaa tttttaattt gataattcta ctaatttttt
     6721 attcattata agtagtataa ttccaaatat caataaaaat gatggtatca ctatgaagac
     6781 ttgtaacata ccaagttggt ctataatagc ttgcgtatag ccagataatt tatttgctgc
     6841 aaatttgcct aaaaaccaaa cacccattag gaatgaaata tacttttcag gcgaaagttt
     6901 atttaccata ccatacccga ctggagaaaa gcaaatttct cctatacttt ggaatgttaa
     6961 aaatccaact acccaaaata cacttgcttt gtgaattgaa ttagggtctg ttcctgtttg
     7021 tataactgct actatcataa acaaaaatgc tatagctaat agtataaaac ctaagctcat
     7081 tttttttggc acacttaaat cgcctttttt agtttgagat aattttaccc atactaaagc
     7141 agagataggt ccaagaataa cacaaaggag accattgtat gaatctatcc atgaagtagg
     7201 aatttgaaaa ttgccaacag taagatttat gtgttccttt gtatagagtg caatagacat
     7261 agaggcttgg ttatatgcta tccaaaaaaa tactgtgaaa aaagctaaca ccaatattac
     7321 tattattctt tttttctcaa tcaaagttaa aggtcttgaa gctagcattt tcttatattc
     7381 tagtgatttt cttatagact ctctcccgtt actagggtca ttattcaaag gtttataaaa
     7441 ctttctgttt ccaaatataa agaagaataa acctattaga accgtaattg atgaaaataa
     7501 aaatacatat ctatatccat acatacttat agagaatccg cttttcatag caaaccattc
     7561 attagcaact aaaccgcata ttaaagggcc aaataaggta cctagattag tagccatata
     7621 cgtaatagag aaagctccat cttttcttgg gtcatcattg gtatataagt ttccaaccat
     7681 agtatgtaag tttcctttat aaaaacctat tcctaagcca gataaaaata tcatgatgtt
     7741 tatatccatc ttactatttg ctatgtatcc tagaaaataa ccaattgaaa ccattagcat
     7801 tccaagtata atacagtttt tgggacctaa aaatctatca ctgacccatc caccaataac
     7861 tggagagaaa taagtccatg ctaaaaaaga tgcaagcatt gatgcggatt ctgctggtgt
     7921 taaccctaaa ccacctttgg agacttgttc agctaaaaac aataataata ccaatttcat
     7981 accatagtat gcaagtccta caaaggtaaa tattgaacag cagaaatata aatctctagg
     8041 ttgtttaagt ttttcttgcg tttttaaaac tgacatatgt cgagaacctc cagataaata
     8101 gtttaacaaa attttaccat actatatgag tatatgtttt tttcatatag acgtcaactt
     8161 gtaagataat ttaattaagt cgaaatatat gtaaaaatag tatcattttt gtgtattcag
     8221 taatattttt ttatgataat atatgttatt attatattaa aatacaaaca tatttatgga
     8281 aaggatatgc caatttagta gttagattga ttgtttggca ggtgggatga gctatgataa
     8341 gaaagaaatc tgaattacaa gtagaagtaa aagaaaattt aaatggtggt ataggaaagg
     8401 ttaagcttga aaacataata caaaatgaag agcttaaagg aaaaggaaga ctatttaaaa
     8461 gagtagcttt accagtagga agttcaattg gtgtgcatga ccatacaaca gattttgaag
     8521 tatattatat actaaaggga aaaggtaaag tatttgataa tggggaattt gtagaagtaa
     8581 atgagggtga tgtagtgtat acagctgatg gcgaaaaaca ttctatagaa aatataggag
     8641 aagaagatct tgagtttgta gcactagttt tatatatgta aaatatggag gaggagtcat
     8701 gacaggcaag atagaagaaa ttaaggacaa gataaataga ttttgcaact ccaatttaaa
     8761 tcaagaatac aaagatataa gctttaaaat tttgcaaaac cttttagata ataataaaga
     8821 gataattcat tcttctagag ctgatatatg gtcttcagct atactaaata tagttttaga
     8881 acaaaatctt ttatacaata agaagcatcc actacatata acaaaaaaag agttttcaaa
     8941 acaaataggt gtttctttaa atactataaa taataaatcg tctttaataa aggatgtttg
     9001 tagtattgat tttttaaatc aagatactat agctatttct aatgtagaat tttgggtaaa
     9061 agagaccaat tccaaatcaa agtctgtaga tttagattta gaaaagaaga aaatactata
     9121 taaaagatat attaagctat cacaagatgc gaatagtgat attgaatcaa ttagatatat
     9181 ggaggaagct gtaaatatag gtaaaagtat gattactaaa gaagataaag aacttggatt
     9241 ttggaaaggt ttatctacca gagcctatat gatagcactt gaatcccttg ctagaaagtt
     9301 agaaagtatt aataatctaa aggagtccaa aaaagtatta gaatatttaa tagaaattaa
     9361 tcctagtgat gagcagggaa taagatacaa attattaaat gtattaatta gattaaatga
     9421 tagaacttca ataaataaat tatttgaact ttataaagaa gaaaaatctg ctacatggat
     9481 gtacagtaaa gctctttatt actttaaaaa taaaagtatg tttttggcaa atgattctat
     9541 taaggttgct agaagtaaaa ataagtacgt tggattatat ttgttagatt ggcgaaatgc
     9601 ttttggtaga gaatttgtta cagaggaaga aaaggcagaa gctgtctatt actatgatga
     9661 aaacatagtg atttggaatg aagttaaagg ttctatggat tggctattaa aaaaaatgtt
     9721 ggaattttca taaaaaaaac tactccttac ttttatataa gtaaaaggag tagtttttaa
     9781 tttatacatc tagttttaaa tcttcaggtt ttattagacc tttttttctc ataaattctg
     9841 aaatcaacaa agttacaata gcaggaagta taaaattaag taaaagtata ccaacccaca
     9901 tagatatacc tccttttcca gcactttgca tagcagttac tgttccaaat tgtccaacca
     9961 atccacaagt gcccatacca gaaccaatag gaatattctc cattttgaat attgttgtag
    10021 ataatggtcc aagtaatatt gaagctacag taggtggtat ccaaattttc caatttttta
    10081 ttatattagg tacttgcaac atagatgtcc caagaccttg tgccacaagg ccaccaactc
    10141 cattttctct aaagctcata acagcaaagc ctaccatttg gcaacaacaa cctacagttg
    10201 ctgccccacc agcaagacca cctaaactta acatcataca aatagctgca ctacttatag
    10261 gtaatgtaag agctattcca acaagtgcag agactaaagc tcccattatt ataggttgca
    10321 tagttgtagc ttgcattatt atatttccaa atgaagtcat aaaagcagaa acagctggac
    10381 caataagttc agctacaagt actccaatta gtattgtcac agcaggtgta accaatatat
    10441 ctatttttgt ttcttttgaa actaattttc caacctctac agcaaaaagt gtagcgacaa
    10501 atgctccaac aggtcctcct aattcattac cacaagctcc aacaagtgca caagaaaata
    10561 gtacaagtgg aggtgcttga agtccatacg caatagccac tgctatagct ggaccagtaa
    10621 cctgtttagc aagagggctt ataacttcag taaaaagaga aatattgaaa ttatctccta
    10681 atgtattgaa aattgttcca attaaaaggg acgaaaaaag accgagtgcc atataactta
    10741 gtgcgtcaat aagatacctt tgaacagaaa tttcaatatt ttttctttta gcaaatgatt
    10801 tgaaatttct ttttttataa ttgatttcca atttaaaatc ctcctcatat aatataagta
    10861 taatttcttt ttaattaatt atataacttt tgagctgaca agacaaatgt tatttcacta
    10921 aaattagact attgtaatag taagaaatta acattaattt tagttttaaa ttttaaattt
    10981 atggtttata attagtatag ggatattctg aaatataata attttccagt ttatcccaat
    11041 atttaactta ggtagattgc ttacaaaaca aatgattata tattaggcta aaaggaagtg
    11101 gatagatgat atttgtaaaa aataagaaat acagaaaaat tgccataatt atatctattc
    11161 ctatattgct tgttgcgcag tactaccttt ttaaatttgg aatacttaga ccaatggtaa
    11221 agggagtaga aattgaaatt gtagatgggg aatatgttaa agatatagat aaatatgtag
    11281 taaaattaaa tgaaacagtt actttgtcta caggtgaata tattaaggtt ccttcgtatg
    11341 ctaaaaatcc aaatatttgg tttaatgtat tggataactc agggacttta aaaatagatg
    11401 gaaataaaat gacagcatta aaggttgggt attcttctgt gggtatcatg aaaaattcaa
    11461 gagtattaaa aaaggctact ataaaagtag tagacccaga ggtagaatca ctagatatgg
    11521 agattaatgg ggatttaaaa catgttggtg atagtgctac tatagaaagt acaatagaag
    11581 ttgattatga taagtttaaa aaatcatata agccagttta taagagtagc aatgaaaata
    11641 ttttaaaaat caaaggaaat aaagtaagtg cagttggagt tggtaaagct acaatatatg
    11701 ctaagtgtgg aaataaagaa attgagagaa catttaggat tcaagctaga gttgctgaaa
    11761 taaatatgga tgatataaaa atagaagttg accagaatga aaaattgaaa ccagatataa
    11821 taacttctcc acctggttta gaaccaccaa aaatatatta tgagttagta gattcaaaat
    11881 tacctgtaga aagagcaata agtttaagta gtaatggaaa tgttgtaggg cttagagaag
    11941 gtaaagatag ggtgagaata acttgtggag ataaatctaa agtaataaca gtaacagtag
    12001 tcaaagaaac tataacgaat gactatatac aaaacttggt tggttcacat aaaatagtaa
    12061 acaataaagt tactattaaa cttgaatggg attatatgaa agatgtcttt gattatgata
    12121 tatatcttaa agataagttg tcaggagaat catttaagaa aattaaatct ataactataa
    12181 atgaaagtga tttaggtaag tctaacaagg tatatgcgac tatagaagta gatatgaagg
    12241 gtaatcaaaa tatagatttt gatatttatg tcataggtaa aaaggattca gatacaacta
    12301 agccaagtaa tgtaataaat attaccaatg gaaatggaaa ttcaaataat gataataata
    12361 ttaaagatat gagggtagaa aatatatcag ctaatgttga tagagataat aatgtcatta
    12421 gagttaactg gagtagtata tctcataagg actgtactta cagtatatat gtaaaaaata
    12481 atactaatgg agatggagaa tttactctat atcaaagtga tatacaggga aatgagcata
    12541 ctataagtat tcctgaagga gatttaaatc tagatattta tgttgttgca aattataatg
    12601 gaaatagctc tcaaaatagt gatgttataa atgtaagata gatttgtata caaattgaaa
    12661 gtgttgagtt tatttaaata ttaaattaag aaaaaggata gtatagggta attatactat
    12721 ccttttttgt tacaatatat aggtttaaaa tctctagtaa tataaattat aaaaagtctt
    12781 atttacacaa atctatagat atttttttta ttaatcaata ttgtcttaaa ttgaacttag
    12841 tgcagcttct attgcatgta ttactgtcgt atcaaatagt ggaatagaag tgtcattttg
    12901 tttaattaaa agacctattt ctgtacatcc taatataaca gcctctgcac cttgtttgcc
    12961 aagcttatct ataatagata agaaagcttt ttttgaagat tcagatataa ttcctaagca
    13021 aagttcatta aatattgtgt catttactat tatacggtct tcttcatttg gaattaagac
    13081 ttcaattcca ttattaataa ttacattttt ataaaaatct tgttccattg tatatttagt
    13141 acctaataag gccacttttt ttatttcttt ttcccttaat accgtagcag ttacatctgc
    13201 tatgtgaagt aatggaatat gtatactttc ttgtatttta tctgatacct tatgcatagt
    13261 attagtacag ataacaataa aatctgcccc tgcctcttgt aatttaattg cagcatcagc
    13321 taaaattttg gcgctttttt cccaatttcc actagattgg cattcttcaa tttcttgaaa
    13381 atctacactg taaagtatac atttagaaga gtgtagtcca cctaatcttt cttttattac
    13441 tgtattaata acttgataat atgtaatggt actttcccaa ctcataccac caattaagcc
    13501 tatagtcttc atattatacc tctcttattt atttattttg aaatagctat ttacaaatac
    13561 aagtatagca tctaaaatag aagcatacaa taataaaatt gtatggagta caattataat
    13621 taagaggagt aattatatgc caataaattc ttttgaaaac tatcctatga catggaaacc
    13681 aaagttagat aaccgaaaac caccaatata taaaacttta gcaatgttat tagaagaaga
    13741 tattaaaaga ggaaatttaa atcctggaga taaattgcct ccacaacggg aattagcaga
    13801 ttttctagat ttaaacttaa gtacaataac acgagcattt aaactttgcg aagaaaaggg
    13861 actaatatgt gcaaaagtgg gaaagggaac attcatttct tctgatgtaa atgtaagtaa
    13921 tacattatta tatcaaacgg aatcaaaaga cataattgag ttggggacag ttcatcctcc
    13981 gtatgagcaa aatacatata ttattgactt tattaaaaat gtactaaaac aaccagaaat
    14041 ggataggttt ttacaataca tgtctccatc tggcacatat atgcaaaaaa agtcaattgc
    14101 aaaatggata gaaagaaata atgtatacac cagtgaagaa aatatattat tatctacagg
    14161 agggcaaaat gctatatgtg caactttact aggattattt aaagctggtg atagaattgc
    14221 tacagattct ttaagttttt ctggaattaa gtcaatagct aaaatgatag gtattcaatt
    14281 agtcccaatt cctcaagaaa ataatgaaat atcgattgaa tatcttgaaa gttattgtaa
    14341 aaatgaaaat ataaaaggca tatatcttat accagattat cataatccaa cgacacatac
    14401 tatgtctgat ttatctcgta aaaagatagc caagatagca aagcaatata atataataat
    14461 tattgaagat gctataaata gcatttttag agatggtata caaactccca tattttcttt
    14521 agctagtgat aatactatat atatttttag tacatctaaa tttttatgtg ctggtcttag
    14581 agtagcattt gtagttgctc caaaacgata tatagaaaat ttagaaaatg cactatataa
    14641 tatgaatcta atggtttctc catttacagc agaaatagta caccgtttat tatattctcc
    14701 tattatagat aaaattatag aagagaaaag agatgcaatt atagaaagaa atgaagttgc
    14761 tgataaagtt ttgtcagatt ataatttgat tggtgataag aattgcagtt ttcgttggtt
    14821 acttcttcct gatgaattgg atggaaaatc atttgaaatt tctgcaaaaa atctaggagt
    14881 tcaagtatat tgtgctgaac gattttcagt aggaaactct acagttccaa aagcagttcg
    14941 tatttgtgta acagctccaa aagatgtaga agaattagaa aaagggctta atataataaa
    15001 gtcacttctt aagagttaaa tttattttac tattatttaa ttaaataaat aaaaaggcgg
    15061 agaaatttat tataataaac ctccgctttt attatttaat gaaatacttt actttttatt
    15121 aaaaaattta attttaataa tcttgaaaat ataatcgttt tatggtaaaa taattcttaa
    15181 aaatagaaaa aattaatcat ttgttaaaaa aaatgcataa tttattgtaa tttattgacg
    15241 aaggttgaaa aatattcata aatgtgcgaa ataacagaaa aaatgcaaaa attaattcaa
    15301 tttcatttgt tataaaatct atatggcaaa attaaatctg cctatcagaa tgatataaac
    15361 acatcagaaa ggaggacgct atcaaataat acaagtagtt aactttgata gcaaaaaatt
    15421 atgaatgaac aaactagaat atccttagag agagctgctg aattaaaatc aaaaattgat
    15481 gattatattc aggctagaaa aacgattaac agaggtcttg aaaaagaaga agagataaat
    15541 aaacgaaaac agaaaatatt aagtatctta aatggaactg aagaggattg gaataactac
    15601 aaatggcaat tatcaaatag aataacagat gtagatactt tatcaaaaat tataactcta
    15661 actaaaaaag aaaaagaata tataaaagag gttggtactc aatttagatg ggcaatatct
    15721 ccatattatt tgagtcttat agacccagaa gatatatgtg acccaataaa attactgtct
    15781 ataccaacac atatagagtt ggaagatgaa caagaagatt tggacccaat gggagaagag
    15841 tatacaaacc cagcaggatg tataactaga agatacccgg atagattaat aataaatgta
    15901 acaaatgagt gtgctatgta ttgtagacac tgtcagagaa gaagaaatat tggacaacaa
    15961 gattctcata agtcaaaagc tattatccaa gaatctatag actatatcag agaaaatgaa
    16021 gaaataagag atgtactagt aactggtgga gatgctctta cattaaaaga tgattattta
    16081 gagtggattc ttagccaact taaagagata ccacatgttg attatgttag attaggtact
    16141 aggactcttg ttacaatgcc acaaagaatt acagatgaat tttgcaatat gctaaaaaaa
    16201 tatcaccctg tatatataaa tactcatttt aatcatccaa tggaaataac taaggaatct
    16261 aaagaagctt gtgaaaagtt agcaaatgca ggagttccat taggaaatca ggcagtatta
    16321 ttaaatggaa taaataatga taaatttgta atgagatgtt taaatcaaga attactgaaa
    16381 ataagagtaa aaccttatta tatattccaa agtaaacatg taaagggaac aaaacatttc
    16441 aatacatcag tagatgatgg tcttgaaatc atggagtatt taagaggata tacatcagga
    16501 atggctatac caacatatat agtaaatgct ccaaaaggag gaggaaagac tcctttgctt
    16561 cctcaatacc ttgtatcaaa aggaacagat tacgttatgc ttagaacatg ggaaggaaaa
    16621 gttataaaaa tggaagatga acctgctgta gatataaaga aacttataaa agaacaagca
    16681 caggattaaa aaaatattta aagtttataa aaagaaacaa ctttaaaata aaaagaaaat
    16741 ggatgtcaaa tttatatcaa tttgcatcca ttttttatta gttatttatt attttgttgt
    16801 tgattaacta attcaagata tccacttggg tggaagtaat atatctcaga agtattttca
    16861 tttacaaagt aaccaatatc accatctgca tctggtaaaa atacataacc attaagattt
    16921 ttagctttta atgcttcaaa atcactagca tttccttgat atatgtaatc aacttcattg
    16981 ttgtatttta ctaaaagttc tttagcttgt tcttgagtca atttagtatt ttggcttaca
    17041 ggaacattct tttgagaatc tgcataagaa gattcattta ctggtgcagt taataacaaa
    17101 caagataaaa ctaataaaga tataagtgct tttttcatac taaaaccctc tttcatgttt
    17161 aattatataa gattcattac ttaataatat tgtatgtttc ctataattaa atattaccat
    17221 taaagagaat aatattacag ttacacagct gtaacttttg attacaaaat cgtaattaaa
    17281 ttgtattttt attccatttg tattttatca tgtctacgat ttacaaactc acagaagtat
    17341 atctttataa cgcccatcac aggagcagat agaatcatac caggtatacc aaagaatcct
    17401 ccaccaacag taactgctag gattgtaaag aatgggctaa gacctagttg tccacctacg
    17461 atttttggct ctataaatgt aacttcaact tgttgaatta ttattaagta aattaaagaa
    17521 gttaaagcaa ttgttggatt ggagaataaa ttaattatta caactggtgc catacctatt
    17581 actggaccaa aatatggaac catattcatg acacccataa gtgtaccaaa taatagtgca
    17641 tattcagatt ttaaaaagta aagaccaatt ccagaaagta ctccaactat aaaagaatct
    17701 aaaatcttac ctgtaaagta tttaccaata tttaaattta agctttgaca tagttctatt
    17761 ataaagtctc catatttttt acctaaggaa atataaacta ctttttttgt aaacagtaag
    17821 aatttttctt tttctaataa tatatagaaa caaattataa aacctaaaag aaattgtact
    17881 ataaattttc caactgaaaa tgtagtgcta aatatcccac caagtgaacc aataagcaag
    17941 ttactaaatt taggcattac actcataatt ttgtcaccat actcttttaa tgtattaggg
    18001 tcaactgatt gtaaactctt acctaagtcg aataagaatt gctctgtttt atctatgtat
    18061 gtaggaattt gagcaactaa atcagctaaa ctatttacta tggatggaac tgtaaataat
    18121 ataaaggaaa ttagtataac tatgagtaca ccataagtaa gaagtaacga atatattctc
    18181 ttaaaattta atttgctttc taagaacttt actagtgggt tgaatatata tgctaataca
    18241 aatgctatta taaatggtgt tagtagtgac attaataaac ttatcactcc aaaaaaatat
    18301 ttgtaattat caataacttt aatcaagata tatgaaacaa ctacagtgag tacaatattt
    18361 agataaattt ttttatcttg caaaataaca caacctttct tatatttatt aaatttagat
    18421 atacttaaat ctaagttaaa gttaatattt atagcttatt atacaataaa aagcagtatt
    18481 aatatagtat
//
LOCUS       DAEMIK010000083        18085 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEMIK010000083 DAEMIK010000000
VERSION     DAEMIK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.083"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..758)
                     /locus_tag="KPK87_002324"
     CDS             complement(<1..758)
                     /locus_tag="KPK87_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2538469.1"
                     /translation="MIDKILKDIKGLFKVQDKAKFLKQNIPYLAFFYVGNIFSHHVRA
                     YTGGDVIDKIFQGILELNTMSFLPSFHPTDILIGVGVATLIKFIAYTKGKNAKKFRQG
                     KEYGSARWGTRKDIEPYIDEKFQNNILLTQTERLTMNGRPKNPKYARNKNVLVIGGSG
                     SGKTRFYVKPNLMQMHSSYCVTDPKGTIVLECGKMLEDNGYEIKILNTINFKKSMKYN
                     PFAYLRSEKDILKLVQTIIANTKGEGEKAGEDFWV"
     gene            complement(755..1240)
                     /locus_tag="KPK87_002325"
     CDS             complement(755..1240)
                     /locus_tag="KPK87_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG2538470.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKGLLNLLKKLMKEAEKFGGLEK
                     LVNVNGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(1242..2252)
                     /locus_tag="KPK87_002326"
     CDS             complement(1242..2252)
                     /locus_tag="KPK87_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2538471.1"
                     /translation="MYLFDEQPIVANKTLARELGLNEALVLQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNSAYISIHPSIKENNQLFSENMKGGRIDISYKNIYQ
                     NCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKISVQVV
                     KERFRKLNSGHLEYLVGVLRENEYKIRNIRAFVLTAAYNAPSGIDAYYSALVSYDMRG
                     GY"
     gene            complement(2353..2871)
                     /locus_tag="KPK87_002327"
     CDS             complement(2353..2871)
                     /locus_tag="KPK87_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBG2538472.1"
                     /translation="MRVYENKEELKTEINKTFEKYIAEFDNIPESLKDKRIDEVDRTP
                     VENLAYQVGWTTLVLKWEEDERNGLQVKTPSDEFKWNQLGELYQWFTDTYAYLSLQEL
                     KSKLNENINSIYVMIDSLSDEELFKPHMRKWADDATKTAVWEVYKFIHVNTVAPFKTF
                     RNKIRKWKKIVL"
     gene            complement(2912..3193)
                     /locus_tag="KPK87_002328"
     CDS             complement(2912..3193)
                     /locus_tag="KPK87_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538473.1"
                     /translation="MRWILKIILFPISLLLSILTAVLSFLLGIGTVILYLLMMFCIFG
                     AIASFLQKEVTIGIEALIIGFLLSPYGIPMVGASIIELLQGINEKIMSV"
     gene            complement(3212..4846)
                     /gene="rlmD"
                     /locus_tag="KPK87_002329"
     CDS             complement(3212..4846)
                     /gene="rlmD"
                     /locus_tag="KPK87_002329"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG2538474.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     MREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(5021..6781)
                     /gene="pyk"
                     /locus_tag="KPK87_002330"
     CDS             complement(5021..6781)
                     /gene="pyk"
                     /locus_tag="KPK87_002330"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG2538475.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(6833..7792)
                     /gene="pfkA"
                     /locus_tag="KPK87_002331"
     CDS             complement(6833..7792)
                     /gene="pfkA"
                     /locus_tag="KPK87_002331"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG2538476.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(8001..11570)
                     /locus_tag="KPK87_002332"
     CDS             complement(8001..11570)
                     /locus_tag="KPK87_002332"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG2538477.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDKF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(11690..12673)
                     /gene="whiA"
                     /locus_tag="KPK87_002333"
     CDS             complement(11690..12673)
                     /gene="whiA"
                     /locus_tag="KPK87_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG2538478.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKNHSNKLRGQSQNKH"
     gene            complement(12690..13115)
                     /locus_tag="KPK87_002334"
     CDS             complement(12690..13115)
                     /locus_tag="KPK87_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2538479.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(13184..14290)
                     /locus_tag="KPK87_002335"
     CDS             complement(13184..14290)
                     /locus_tag="KPK87_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG2538480.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(14302..15159)
                     /gene="rapZ"
                     /locus_tag="KPK87_002336"
     CDS             complement(14302..15159)
                     /gene="rapZ"
                     /locus_tag="KPK87_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG2538481.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(15190..15903)
                     /locus_tag="KPK87_002337"
     CDS             complement(15190..15903)
                     /locus_tag="KPK87_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG2538482.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(15907..16821)
                     /gene="murB"
                     /locus_tag="KPK87_002338"
     CDS             complement(15907..16821)
                     /gene="murB"
                     /locus_tag="KPK87_002338"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG2538483.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(17094..17864)
                     /locus_tag="KPK87_002339"
     CDS             complement(17094..17864)
                     /locus_tag="KPK87_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG2538484.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         5284 a         3583 c         1999 g         7219 t
ORIGIN      
        1 acccaaaaat cttcccccgc tttttcaccc tctcccttag tgtttgcaat gattgtctgc
       61 accaatttaa gtatgtcttt ttcactgcgg aggtaggcga atggattgta tttcatagac
      121 tttttaaagt taatggtatt aaggattttt atctcatatc cgttatcttc aagcattttg
      181 ccacactcaa ggactatcgt tccctttgga tctgttacgc aatagctact gtgcatttgc
      241 attaggttcg gcttgacata aaaccttgtc ttgccacttc cagatcctcc tatgacaagt
      301 acatttttat ttcttgcata cttgggattt ttcggtctac cattcatagt taatcgttct
      361 gtttgcgtta gcaagatatt gttttgaaac ttttcatcaa tgtacggctc aatatctttt
      421 ctagttcccc atcttgccga gccatactct tttccctgcc taaacttttt cgcatttttc
      481 ccttttgtat aggcaatgaa tttgattaaa gtagctactc ctacgcctat tagaatatca
      541 gttggatgaa aacttggaag aaagctcatg gtgttaagtt ccaatatacc ttgaaatatt
      601 ttgtctatta catcgccacc agtatatgct cttacatgat gagaaaagat attgccaaca
      661 tagaaaaatg caagataggg aatgttctgc tttagaaact ttgccttatc ttgcacctta
      721 aataagcctt tgatgtcttt tagtatctta tctatcatag gctttgctcc ttttgtttat
      781 ttttgatttt ctcttttgaa atagagtttt tagccatctc tttgaacttc tcaatattct
      841 tgtgaataga ttctttcctt tctgctttct tttctgattt tgaaagagcg tgcttaaaag
      901 ctttatccat aactttcata tctttggctt gaaagaatac agagtttttt cctgtctctt
      961 tatttttcat cactgaaaat ttaacaccgt atctattcag ctctttttta agttctttaa
     1021 gctcagcttc ttccactggg atttcttcaa gctgtccctt tttaaccata tcttttagct
     1081 ttacttcatt tccgttgaca ttgaccagtt tttcaagtcc tccgaatttc tctgcttctt
     1141 tcatcaattt ttttaataag tttaatagac cttttccagt aactttacca gcatttatct
     1201 ccatatttaa ggtctttctt gctatttctt cattgatcac atcaatatcc tcctctcata
     1261 tcatagctta caagagccga ataataggca tctattccac ttggtgcatt ataagcggcg
     1321 gtcagtacaa aagctcttat atttctaatc ttatattcat tttctcttaa tactcctact
     1381 aagtactcta aatgtcctga attaagtttc ctaaatcttt cctttactac ttgtacagat
     1441 atctttgttt ggttgatagt aataaaagat tcatcatcta ataccattac atctgccagt
     1501 atctttaata tttcatcaaa ctcttcagcc acactcttat tcccaatgct catatgttca
     1561 tagtatcctg tttggtgtcg caattcttct aaacaatttt gatatatatt tttatagctt
     1621 atatctatcc tccctccctt catattctct gaaaataatt gattattttc tttgatggaa
     1681 ggatggatgg aaatatatgc tgaattattt gtagtattat ttgttgtatt ctctggtaat
     1741 ggttggtgca aattatcctt ttgcattagt gcattttgaa cctgtgcatt agtgcaatct
     1801 gcattattgc attggctgat tttgccctta tccattggtg cattttgccc taatgcattg
     1861 ggcatagaat tttttaatgc ttctgtttgt attttttgtt tttttaaatc catatccata
     1921 tataattctt caagtttatc attattaatt gtataccatt ttgttttatc tcttggatcc
     1981 ttattgtaat ttccaactaa aagataacct gctttttcta actttgaaaa agttcttttt
     2041 actgtatcaa agctcatata gtcaaagtca ttttcttgcc atgctcgaat tgaattatat
     2101 gtccagtatt tcccatcatg gtaattcttt cccgattttt tatttatttc tatccaataa
     2161 ttgatctgct gtagtacaag cgcttcgttt aatcctaatt ctcttgcaag agttttattc
     2221 gcgactatag gttgttcatc aaataaatac ataccatcaa acactccttt cttttttcaa
     2281 ataaaaagac gatagttttt ctatcgcctt tgataaccca tttatgaaat ttttaacttt
     2341 actgtctgta atttataata ctattttctt ccattttcta attttgttcc tgaaagtttt
     2401 aaaaggagct acagtattca catgaataaa cttataaact tcccacactg ctgttttagt
     2461 cgcatcatca gcccattttc tcatatgtgg tttaaataat tcttcatcac ttaatgaatc
     2521 aatcattaca tatatagaat taatattttc atttaattta gattttaact cttgcaatga
     2581 taaataagcg taggtatctg tgaaccattg atataattca ccaagttgat tccatttgaa
     2641 ttcatccgat ggtgtcttta cttgaagccc atttctttca tcttcttccc atttaagaac
     2701 taaagttgtc catcccacct gataggcaag attttctacc ggcgttctgt caacttcatc
     2761 aattctttta tcttttaagc tttctggaat attatcaaat tctgcaatat acttctcaaa
     2821 tgttttattt atttcagttt tgagttcttc tttattttcg tatactcgca taaaattacc
     2881 tccacaactt ctgatttatc aattctctga tttaaactga cattattttc tcgtttatac
     2941 cttgcaataa ttctataatt gacgctccaa ccatcggtat cccataagga ctaagtaaaa
     3001 atcctataat taaggcttct ataccaatgg taacctcttt ttgtaaaaaa gatgctattg
     3061 ctccaaatat acaaaacatc atcagcaagt acagtattac tgttcctatg ccaagtaaaa
     3121 atgaaagaac tgcagtgagg atacttaaca acaagctaat tggaaataag ataattttta
     3181 atatccatct cataaatacc ctccatttct actaaatcat tttaaaatgt cttaaagcat
     3241 ccatgatagc ttcttctttt tcaggcgtac actgtgggat agcctgatta tcttttttag
     3301 atttattata atgttctctc atttcaatcc cacatttctt tttaatttgt gcaatataaa
     3361 gtgtagaaac ttttagtcca aatttttcta atacatactc cttgatttga gcgtatgttg
     3421 ctttactctc tgcacttgtc aaatcaagct catccagctt aatttcaaca tctatatgct
     3481 tatcgacatc aagtttggac aataacgcta ccgtctccac atgtgtagaa tgagggaaca
     3541 tatctacagg ttgtgcctca acacatttaa atccgtgaac atttagatag tctaaatccc
     3601 ttgctaaagt agaaggatta caagaaacat agacaattct ttccggattc attgaaacaa
     3661 tagtctctaa taccttttcg tcacatccct ttctaggtgg gtctacaacg actacattgg
     3721 cagtcttacc ctctctatac atcttaggta caatttcctc tgcctttcca acataaaatt
     3781 cagcattatg tatattgttt aatttagcat ttattttagc atcttttatg gcatcatcta
     3841 taatttctat tccataaacc tttttagcct tttgtgccaa aaataaagat attgtaccta
     3901 tcccacagta aatatcaaaa acagtatcag tatcatttaa atttgcatat tcaagagcct
     3961 tatcatacaa tactttagtt tggacagggt ttacttggaa aaatgataat ggtgaaatct
     4021 caaacactaa gtcacctata taatcgttta tttttccatc tccatatata actatatttt
     4081 ctcttcctag aatagcattc gtatcttttt gatttacatt tacaaccaaa gtcttaaatc
     4141 caggtatatt ttccttaagc actgaagcta attctttaag atatggaagt ttgtttccat
     4201 ttgctactaa tacaaccatt acttctttag ttgtaaaacc tatttttgtt actaaatgcc
     4261 taatcaaccc cgtgtgagtc ttttcatcat atatacttac cttataggcc cttatatatg
     4321 tttttattat tttaacaatt ttatcattta tctcatgttg tattacgcat ttttctgtcg
     4381 atattacatc atgagtcttc tttttataga agccaataat tggaatacca ttattctttt
     4441 gtattggaaa ttgtgcttta tttctatatc tatatggatt atccattcct atagtgtcat
     4501 gaacttctac atcttctaac ttacctattc tcgaaagtac ttgtttaact tcatttgttt
     4561 tcatttcaag ttgtttatta taatctaagt cttgtatctg acaccctcca caatccctaa
     4621 gattgtcact acacactctt ttcaccctga atggagactt ttctattatc tcaacaatat
     4681 ctcccacagc ataatttttc tttactttat taatcctaac tttaacttta tcatgtatta
     4741 atccgccctc tacaaaaact gtaataccat tgtactttcc tactcctact ccgccttgac
     4801 caatgtctac tatatctaca atatacatct cgtttttcga aagcataaat caccctccaa
     4861 tcaatctatt atatttaaat ctttacaaac ttttttcttt aaatttatat taaaatattg
     4921 ctattaatta aactatattt tttactaagc tacaatattt tattttacca aaaaaaaagg
     4981 agcttataaa gccccttagt aaataaattt attatttttc ttataaaact ctagttgaac
     5041 ctctatatac cacacctctt gatgcatcca cagttacaac ttctccatct tttactagat
     5101 ttgttatatc agttgcagac acaataactg gtatatctaa atttatacct acaatagctg
     5161 tatgtgatgt cattccacca ttctcagtta ctacagctcc tgctttttct atataattgt
     5221 tcatttcagc atcagtagca gtagaaacaa gtatgtcgcc ttcattaaag tctacacaag
     5281 catttccatt ctttattata cgaactttac cttctactgt ttgagtacct acacctatac
     5341 cttgcacgat ttcttcgctt attacatgaa cctttattaa attagtagtt ccacttactc
     5401 ctactggtac tccagcagtt ataactacta attccccatt atctatataa tcagcttgtc
     5461 tagctgcctc aattgccttc tctataactt catctgtatt tcctgcaaca gaagacttaa
     5521 ctggatatac tccccatgta agagctaatt tattcatagt cttttcatta tttgtagtag
     5581 ctattattgg agattttggt ctaaatttag ataccattct agcagtatat cctgaagaag
     5641 tagatgttat tatagctgat gcatttaaat ctactgctgt tgtacaagtt gcatggctta
     5701 ttgcatctgt tacagtaaca ttgttagtac cattttcctt tagtaatcta tcataatcta
     5761 aagtctcttc tgttcttttt gctattgtag ccatcatttt tactgcctct acaggatatt
     5821 ttccagcagc agtttctcca gataacatta tcgcatctgt tccatcatat atagcattgg
     5881 caacgtctgt aacttctgct cttgttggtc ttggatttct tatcatagaa tcaagcattt
     5941 gagtggcagt aacaactggc tttgctaatt cattacattt ttttatcatc attttttgaa
     6001 ctattggcat ctcttcagtt ggtatctcaa cacctaagtc acctctagcc accattatac
     6061 catcagaaac ttttaatatt tcatctaggt tctctacacc ttcttggttt tctattttag
     6121 atattatttg tatatcagtt gcattattgt tctctaatac ttctcttatt gctagtacat
     6181 cagatgcctt tcttacaaaa gaagcagcta tataatctat tccttgactt attccaaact
     6241 ctatatcact tatatcttta ggagttattg ctggtaaatt tatcttaaca ccaggtacat
     6301 ttactccttt atgattttta actattcctg aattttcaac aacacaaact atatcttctc
     6361 catttatttc ttttactctt aagccaacta aaccatcatc tattagtata gaatctcctg
     6421 atttaacgtc ttcagcaaga cctttatagc ttactgtaca catttcttta gtccccataa
     6481 tatctttcat agttatagtg aacttttgcc cttcttctaa aaatacctct gggtcttcaa
     6541 agttacctgt tctaatttct ggacctttag tatctaataa tatagcaact ggcttattta
     6601 atttttctct aactttcttt gccatatcaa ttctttcttt atgttcttca tgcgaaccgt
     6661 gagaaaagtt aaatctacac acatttagac cattttgcat aagttgagtt aaaacttcct
     6721 cactttgtga tgctggacct agtgtacaaa caatttttgt cttctttaca ttatttaaca
     6781 tatttgctcc tcctgagata ttattccaca gatttatttt taatactaaa acttatatag
     6841 ataatatttt tgccatctca taagcttctt tatcaaatac tttcttttga gctaaagctt
     6901 cacttatttc taaatcaatt attttgtttt cttttattcc tacaactcta gctgattttc
     6961 catctaataa taattctact gctcttactc caagtctact tgctagtatt ctatcagata
     7021 cagttgggct tcctcctctt tggacatgcc ccaatacagt gactcttaaa tctgctcctg
     7081 actctttttt aagttccttt tctaaatcac tagcacttcc gactccttct gctaaaacga
     7141 ttatactatg tctcttacct cttttttgag tagtctttaa tcttagagca acttcatcta
     7201 ctgtaatttc tacttctggt actattatag tttccgctcc tcctgcaaga cctgcatata
     7261 aggctaaatc tccacagtgt cttcccataa cttctactat gttaactctt tcatgagatg
     7321 atgatgtatc tcttatctta cctatagcat ctataatagt attcattgca gtatcaaatc
     7381 ctatagtata atctgtatat gctaaatcgt tatctatagt tcctggtatg cctatagctg
     7441 ggaatcctaa gtcactaagt ttttgggctc ctgcaaatga accatctcca ccaattacaa
     7501 ctaaacaatc aattttatac tttttaagaa tttttactgc ttttaatcta ccttcttctg
     7561 ttttaaattc ttcacatctg gaagatttta atatagttcc tcccctgtgt ataatatctc
     7621 caacagaaga taagttcatt tcagttaaat cttcttctag taatccctta tatcctctat
     7681 tgattccata tactttgcat ccataatata ttgctgatct tacaaccgct ctaatcgcag
     7741 cattcattcc tggagcatct cctccgcttg ttagtaatcc tatagttttc attttttacc
     7801 tccttggatt ttatgtcaat tttttattat aatcattgtt attgtattct aatggacttt
     7861 ttttgtccct atatcacttt ttaatcccat caagactaaa aaaattaaat gtccttaaat
     7921 gtccattttg tgatattata tcacaaaacg gacaatattt ttcatattat tataatatat
     7981 ttagattttt atataaaaaa tcaagctttt acttttatgt tatctttagg aagtaactct
     8041 tctaagttat ttataaattc atcacatata caagtcaatg ccccggtcat tttatatgct
     8101 ttacgtttac catttatatt ttcatccata gggtataagt atatactgtc attacctgga
     8161 tattgtttgg ctatttctac aatattatta gttgtatcag tatcagacat actatctatt
     8221 ttcaaataca attttttacc actttctggc ttaatatttt ctacatttct agttcttcct
     8281 gaattgtagt ttctatctgc aaagtcagta tcatcattta tatcttttat ctctctagct
     8341 ataagttttg cattttcgcc ttcttttata cttaatgttc cttttacata tattatatta
     8401 tcttcattca aaatgacatt atacttttgt aataattgag gaaaaactat tatttctata
     8461 gcaccataca aatcttctaa ttcaataaat gccataattt catttcgttt tgttgtttta
     8521 atagttttag ttacaatcat tccacccatt ataacatctg tttcattcat ttgtaggtat
     8581 gactcttctt cctcttttaa tgaatttaat tttccattat ccatagatgt tttttcttgt
     8641 agttcttttt caaattgtga taaaggatgt ccacttacat acattcccaa gacttctttt
     8701 tcaaggttaa gtctctctct ttcttcatac tcttgccttt taggtaaact gtatatttgt
     8761 tgaaaatcac tatcttcctc actatctcca aaagcatcaa ataaagaaat ttgaccttga
     8821 acatttttct ttctatccat agatacactt tctaataaac tttcataacc agccatcaaa
     8881 ctagctctat tttcacttat attatcaaaa gctccacatt ttattagact ttctattact
     8941 ctcttatttg tatctttttg gtctaatctt ttgactaaat ctactaaatc tacaaataat
     9001 ccattagatt ctctttcagc gattatattg tttattatat ttacgccaac attttttacg
     9061 gctgctagac caaatcttat attgtcgcct tcaactgaaa actttgaaaa acttttgttt
     9121 atatctggag gtaatacatc tatttctagt gctttacatt ctcttatata ctcaactacc
     9181 ttatcagtat ttcccattac actggttatc aatgctgcca taaattcaac tggataatat
     9241 gtttttagat aagctgtctc atatgctaaa accccatatg cagctgcatg tgatttatta
     9301 aatgcatatc tagcgaagtc tatcatatca tcaaatattt tattagctat ttcctcttct
     9361 acaccatttc tgatacaccc tgcaatttct atattaccat cttcatcaaa tttaccatga
     9421 ataaaatatt gtctttcttc ttccatcaca tccatctttt tcttactcat ggctcttcta
     9481 actaggtcac ttcgtccgta actataacca cctaaatctc taacgacctg cataacctgc
     9541 tcttgatata ccaaacaacc atatgtaact tccattatag gctttaattt ttcatgtaga
     9601 tatgttacat cttttgggtt gcttttattt tttatatatg caggtataga atccattgga
     9661 cctggtctaa atagggatat tcccgctaca atatcttcaa atttgtctgg ttttagctgt
     9721 ttcataaaac ttctcatgcc agcactctcc aattgaaaca cccctagtgt atttcctgat
     9781 gaaagcattt catatacttt cgggtcgtca taatccatct ttgaaaagtc tattttttct
     9841 gtgtagtttt ttataactct atttttttct attaaatcaa gagtatctct tataactgta
     9901 agagttctta gcccaagaaa atccatcttt aaaagcccaa gttcttcaag tgttgtcata
     9961 gtaaactgtg ttgtgattgc atcttgatgt ttgtacaaag gtacatattc atctacggga
    10021 ttttttgata taacaactcc tgctgcatga gtagaagcat gtcttagcat tccctctatc
    10081 tgttttgata tatttattat ttcttttgtt tccgggtctt ggtcatacaa ttctttaaga
    10141 ttaggatttg tatctagggc cttatctatt gtcattccta aagcaaatgg tatttccttg
    10201 gctaccttgt caactttatt gtagcctata tccaataccc ttccaacatc tcttattgca
    10261 gcttttgcac ccattgttcc aaaagttatt atctgggcta catggtcatc accatatttt
    10321 ctctttacat aatctataac ttcttctctt ctctcataac aaaaatctat atctatatct
    10381 ggcatggata tacgttctgg atttaaaaaa cgctcaaata atagagagta ctttattggg
    10441 tctatatcag ttattttaag tgtatatgct actatcgaac cagccgcact acctctacct
    10501 ggtcctacta taatattatt ttctttagaa aaatttataa aatcccaaac aattaaaaaa
    10561 tactctacat aacccatttt ttcaattaca tctaattcat aatttaatct ctctagtatc
    10621 tcattgctag gattatcata tctctctttt aatccattaa aacacaactc ccttaaataa
    10681 gtttctggtg tatatccttc tggaacatca tacttaggaa gatgtatagt attaaaatca
    10741 aactccacat tacacatatc agctattttc acagtattgt caatagcctc taatgcatat
    10801 ggaaataatt cttccatttc ttctcttgat tttaaataaa actcatcact accaaatctc
    10861 attctatttg ggtcatttaa tgtttttccc atttgaatac acataagtac atcatgtatt
    10921 ttcgaatctt ctttatttac atagtgaaca tcattagtag caactagtgg tataccagtc
    10981 tcttttgata attttactaa accagagttt acttcttttt gctcaggtaa attgtgatct
    11041 tgaatctcca aaaagaaatt ttcttctcca aatatgtctc tgtagtcaag agcaaattgt
    11101 tttgcttttt cataatttct attcattaaa gcacgtgcta catctcctgc aaggcatgca
    11161 gaaagtgcta taatcccttt gttatgtttt ttaagttctt ccatgtcaac tctaggctta
    11221 tagtaaaaac cttctacgta tgaagttgaa actattttta ttaaattttt atatccttcc
    11281 atattcttag ctaacagaac taaatgtcct tgatatttgt catagtttgg gtctttatct
    11341 ctcaatccac gtgcagctgt atagacttcg caccctataa ttggctttat tccttctttt
    11401 atagctgttt tgtagaaatc tataacacca aacatacaac catggtcagt tatagctatt
    11461 gagctcatgt ttagttcctt agcccttttt ataagttttt tcaccctcga aaacccatca
    11521 agtaaactat actcagtatg tacatgaaga tgagcaaaat ctttgttcat taaatcacct
    11581 cctgctttat aattttaatt ttaccacaaa tatatgtact aaaacaaaaa cagagacttt
    11641 tatctcaaaa taggaattaa cttcaacgta taccattata tgttataaat taatgcttat
    11701 tctgagattg tcccctcaat ttattgctat gatttttttt caaatctgtt gcaatttctt
    11761 ctatttttct aagtctatga ttgactccag attttcctat tggtggttct aacatctctc
    11821 caagttcttt gagagtcata tcctcatact cgattctaat cttagctatc tcttgtaaat
    11881 tttctggcaa actgctaatt cctatagtct cttgtatgag cttaatattt tctacttgtc
    11941 ttactgctgc atttactgtt ttagaaaggt tagctgtctc acaatttact atcctgttta
    12001 cattatttct catttctttt actattttag tattttgaag acttaaaaga gcgtggtgtc
    12061 ctcctataat actcaacaaa tctgatattt gttcactctc tttgatataa acaacgtgat
    12121 tattttttct agccacaatt ttactattaa aacctaaaga gtttataagt tcttttagag
    12181 aatctgcaaa ctcctcatta ttagttacaa attccatatg atagttcttt tctgggtcgc
    12241 ttatagaacc tccaccaaga aaagctcctc ttatgaaagc tctcttacat ccttcatgct
    12301 taattaagtt ctctgaaatt ttgtttatag taaaaaatcc ttctttatct tcaagtatac
    12361 ctaacttttt taataacatt tctgaaccca tttcgcttgt taccattaat acataactgt
    12421 tatttctctt tagcatttga ttcttattta ctgagattgt agtattgata ttaaaattct
    12481 tttttaataa tttaaatact ttacgggcta ttgaatttaa ctctgttgtt atctttagat
    12541 ttagcttctt atatccaaca atttgtatac ttccccctga tttaactaga gctgacaatt
    12601 cagctatatt acaacattca ttcccagata taactcttgc tagttcattt ttggtttcag
    12661 ttgaaaaaga catatctatc acctattaac taatttttct ttaacctttt ttttatttct
    12721 tgtaaggcaa cctttattat ctccttttca tcatcatacc tagctaagtc tactactcta
    12781 tctaggtgga taaatttagc atcaataaag ccttcctctt tttgtggaat agtatccata
    12841 ttctttgctt gcatcaaata ccaaaaaact gttttatgaa ctgctctatt ttcatcccag
    12901 ttttctttga atgtatagtg tatttcacct aaatatttca atatatctgc ttttactcca
    12961 gtttcttcac ttacttcacg taatgctgcc tcttggttat tttccccttc ttctactttt
    13021 cccttgggta acacccaatc tccattgaac ttccttagta aaagaatcgt attgccaaac
    13081 aggactacac caccagcact aacctcttct ctcatcgatg ttcactcctt aaaactaaca
    13141 taatgataag gatttctaca caatttttta aaatcctttt ttactaactt ttgttgtaat
    13201 tatgatttag ggctaactca ataactatgt tagatatata ctttgcatca tgtcttatat
    13261 aattattttt tatttcaatt aaattcttct ctacagtttt tatacccatc tcttttaatt
    13321 tatctttttg ctttttatct aacaagactt gctctgctcc atcttgttta tataagtcaa
    13381 gcatttcttc tggcaatatc tcattatttg cgattacata atctattaaa ttctcatcag
    13441 tgtgttttac tatagcatta acatgctcta atacattgta cccttctgtc tcacctggtt
    13501 gagtcattat atttgctata tatacttttg gagctacaga cgatttaata gcgtctacaa
    13561 tcccctctac aagtaaattg ggtattatac ttgtatataa acttccagga cccataatta
    13621 taatgtcagc atcataaata gatgcaataa catcctttaa tggttttaca tcttttggat
    13681 ttaaaaaaat tttatcaata gagctttttt gaatttttga ctcttcaggt atactagatt
    13741 ctccatttat tatattgcca ttttctaatt tggcaactaa atttacatct tccaaagtta
    13801 ctggtaaaac tcttcctgtt atagcaaaaa tttcacttaa tttatataca gctttttcaa
    13861 agtttccata caacccattc atagcagcca aaaacaaatt tccaaaactc tgacctttta
    13921 aaagcccttc tgtaaaccta tattgcataa cttcattcat tgttggctct atatttgcaa
    13981 gtgctaataa acagtttctt atgtctcctg gtgggagcat tcctaagtct tctctcaata
    14041 cacctgaacc tccaccatca tcagcaacag ttacaattgc agttatattt tgcgtagtgt
    14101 gtttaaggcc tcttaagaat accgattgac cagtacctcc acctatcaca accacatttg
    14161 gttctctatt ttcaacaata gacctttgaa atagttttac tttttcattt tttgcttttt
    14221 tatatataaa taacgataca attaatgcaa tccaacctaa aattcctaca ataataagta
    14281 atttagccat gtttgcctcc attatcgcaa catagagtct ctatgtttct ttacaactct
    14341 atatccctta ttagataaat cctccgctat aagatttgtt atagtaactg acctatgtct
    14401 tccacctgtg catccaacac caattactag atgttgtttt ccttcctcta tatattgagg
    14461 tactaaaaaa tgaatcatat ctaaaagttt cacataaaat tcttcactga ttttagaatt
    14521 cataacataa tctctaactt ctttatcatc accagtttta gctcttaact cttcaacata
    14581 atagggattt ggaagaaatc ttacatcaaa tactaaatct gcatcagcaa gtataccatg
    14641 tttaaatcca aaagatacta cagaaatagt taggtttgga ttatcttcac ctgaagaata
    14701 tattttcttt atttcttcct taaggtcttt aggtttcatg ttagttgtat caattataca
    14761 tgtagataac tcctttaaag gttccattat ctttctttcc atttttatac cttctggaat
    14821 ctgcatatct tttgccaatg gatgatttct tcttgtcatt ttatatctct ttaacaatac
    14881 atcatcatta caatccaaat ataccatttc atattcataa ttttcttttt ctaaataatt
    14941 taagctttca tgaagcgctt caaaaaactt tctccctctt atatctatcc ctagagcaac
    15001 tttatcaata cttgaatttg gttgataaca taattctgca aatttagtta tcaaagcagg
    15061 aggtagatta tctacacaat aaaatcccat atcctctaaa gctctcattg tctcactttt
    15121 tccagatcct gaaagacctg taactataac aaatttcatt gctaactcct cccttttatc
    15181 ttatgtttat tatattataa tgttttctac taacgatata tccagattag agtcagatat
    15241 agtcttcata gctagttcaa aatcaccaac tctttctgga caatgtgcat ctgagcctat
    15301 tataaattta ttttctagcc cttttatttt atttaattga tttacattca aaaaagaatg
    15361 gctactattt atctcaagtc tagtatttgt cttttttgct gtctttgcta cttcttctat
    15421 atacacatcg cctttatctc ctggatgagt aattataaaa atatcattat tacttatcgc
    15481 atttattaca gcttttgtat tatattcttt tgctttttca gttttaaaaa tatagttatt
    15541 acaatgattt aataagcttc ttaatgatgt tggagtagaa ccaaaatgat atccagccat
    15601 aatataatca aaatctctaa taaatttatc atccatatct atatttcctt tatcatctaa
    15661 tatgttacac tccataccta aaagtatatt tatatcagtg tacttatcat ttaaataatc
    15721 tacttcttct ctcattttga gaatatcttt tttctttaca ccatatgcaa tatgtttata
    15781 actatggtca gatattccta ttgtttttat tcctttagaa atggctacct ttacattatc
    15841 ctcaatagtc cctttgccat gactatataa tgtatgtgta tgataatcag ccagtatttt
    15901 catttgttaa tcctctccaa gtattttgac ttcttcttcc aacataaccc caaacttggc
    15961 atatacagca ctttttataa catatattaa atccaaaata tctttagcac ttgcttctcc
    16021 ttgattgact acaaatccac aatgcttctc agatacttgt gctcctctta aagtaagacc
    16081 tctcaatcca gtttcttcta ttaatttacc tgcaaaatat cctgttggtc tcttaaatgt
    16141 acttcctgca ctagcaaaat ttaatggctg tttagttatt cttctcgtcg ctaaatcatc
    16201 cattaaactt tttatttcat cataatttcc ttcttgtaat tctaattctg ctgataaagc
    16261 tatatatccg tttttcgata ttatactttt tctataccca aattccatct gttcatttga
    16321 taattcaaat atattgcctt ccatatccat cagtcttact gacttcacta tgtccttcat
    16381 ttcaccacca tatgctccag cattcatggc taatgctcct cccaatgtgc caggtattcc
    16441 tgatgcaaac tcaaaccctt ttaattcttg cttttggaga gctttcccta aaactgataa
    16501 taaagctcct gactgagcag tcatcctagt atcatttatc tcataagaat tgaaattatc
    16561 agctaattct attaccactc ctcttattcc accatcttta attaaaatgt ttgaaccatt
    16621 tccaattatt aaatacggaa tagattcttt ttttaccaac ttaagtacat tttttaattg
    16681 ttcttcagtt ctaggtctaa ctaatatatc tgctggtcct cctaccctaa atgaaatatg
    16741 ttttttcata ggttcatcga cttttatatc ctctttactt aatatattta ataaatttat
    16801 atatatatct tgattattca ttgtatttcc tcccaaatta tactgaactt tagctgtcat
    16861 tattatttaa attacattag tatatagtat taactatatt caataatata tgattttgct
    16921 tatcatatag attttatatt ttaagcttct taattacaaa ctatattcaa taaatatata
    16981 ataaagtaaa aaaaaaaaaa taacaacata taaaagaata taaataattt aaaaaaagca
    17041 tcaatatcac tgatatcaat gctttaaaag gcaatttaat tcgtattatt tatctaacta
    17101 tttttgacat agcaaagata ataaatccct ccaactacta tggaacctcc aacgatattt
    17161 ccaagtgtta caaataccaa attagttata aattgaacca tagttatatt tgctcctaat
    17221 gccattccca ttggtataaa aaacatgttt gcaacactat gttggaatcc acataataca
    17281 aatagcatta ttactgtcca acatgaagcc aattttgaca ttatatcttg agatgctgta
    17341 gccatccata cagctaaaac gactagaata ttacaaagta ttgcttttaa aaacattgat
    17401 gctatagttg aattaacctt agcttctgcc acagctatag cttttgtagc tgcatctcct
    17461 gtcaatgtag aagaataaaa cacaagtgca actagcaaaa ttgaacctac taaatttgca
    17521 aaataaacca gtccccagtt tcttaacatt tttcctaatg ttattttttt gtccataagt
    17581 gcaatagcca ttaatgtgtt tccagtgaat aattctgctc ctgccactac tacaagcata
    17641 agacctactg gaaagaccat agcacttaaa aagctagcta cccctgggtc tatcttgcca
    17701 aaagtttgac taactatcat actccccaat gccccaaaac ctataaatgc tcctccaaat
    17761 aaacctaata gaattgcgtt ttgtgtcgaa atatttgctt ttttaatgcc tgcacttata
    17821 gttgcttgtg cagtttcccc aggtgtcaag aatcctttac tcataaatcc ttccccctta
    17881 aaaattatac tttcccttgt ctttttatgg caataattat ataataacaa tatgatatat
    17941 aataaatttt attaacataa aaaatatact atatacttat atatacttgt ctcatataat
    18001 tatactattt attatataac aactttgtta atttcgaaat aatttatatt gaatttgaaa
    18061 aatttacctt ttgattattt atttt
//
LOCUS       DAEMIK010000084        17699 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEMIK010000084 DAEMIK010000000
VERSION     DAEMIK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.084"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            125..487
                     /locus_tag="KPK87_002340"
     CDS             125..487
                     /locus_tag="KPK87_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538485.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            471..1187
                     /locus_tag="KPK87_002341"
     CDS             471..1187
                     /locus_tag="KPK87_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538486.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEYEEELKQNIIE
                     NIKKNFDEKNLVQVYKYRGV"
     gene            1207..1626
                     /locus_tag="KPK87_002342"
     CDS             1207..1626
                     /locus_tag="KPK87_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538487.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            1616..2140
                     /locus_tag="KPK87_002343"
     CDS             1616..2140
                     /locus_tag="KPK87_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538488.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            2452..3009
                     /gene="efp"
                     /locus_tag="KPK87_002344"
     CDS             2452..3009
                     /gene="efp"
                     /locus_tag="KPK87_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG2538489.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3101..3556
                     /locus_tag="KPK87_002345"
     CDS             3101..3556
                     /locus_tag="KPK87_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2538490.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            3719..4429
                     /locus_tag="KPK87_002346"
     CDS             3719..4429
                     /locus_tag="KPK87_002346"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG2538491.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            4483..5553
                     /locus_tag="KPK87_002347"
     CDS             4483..5553
                     /locus_tag="KPK87_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2538492.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            5581..9135
                     /gene="smc"
                     /locus_tag="KPK87_002348"
     CDS             5581..9135
                     /gene="smc"
                     /locus_tag="KPK87_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG2538493.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLESSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            9162..10442
                     /gene="ftsY"
                     /locus_tag="KPK87_002349"
     CDS             9162..10442
                     /gene="ftsY"
                     /locus_tag="KPK87_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG2538494.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMNSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            10545..10913
                     /locus_tag="KPK87_002350"
     CDS             10545..10913
                     /locus_tag="KPK87_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG2538495.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            10910..12262
                     /gene="ffh"
                     /locus_tag="KPK87_002351"
     CDS             10910..12262
                     /gene="ffh"
                     /locus_tag="KPK87_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG2538496.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            12300..12572
                     /gene="rpsP"
                     /locus_tag="KPK87_002352"
     CDS             12300..12572
                     /gene="rpsP"
                     /locus_tag="KPK87_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG2538497.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            12637..12864
                     /locus_tag="KPK87_002353"
     CDS             12637..12864
                     /locus_tag="KPK87_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG2538498.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIILKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            12972..13487
                     /gene="rimM"
                     /locus_tag="KPK87_002354"
     CDS             12972..13487
                     /gene="rimM"
                     /locus_tag="KPK87_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG2538499.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            13496..14191
                     /gene="trmD"
                     /locus_tag="KPK87_002355"
     CDS             13496..14191
                     /gene="trmD"
                     /locus_tag="KPK87_002355"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG2538500.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKYIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYILTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            14376..14726
                     /gene="rplS"
                     /locus_tag="KPK87_002356"
     CDS             14376..14726
                     /gene="rplS"
                     /locus_tag="KPK87_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG2538501.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            15047..15943
                     /gene="ylqF"
                     /locus_tag="KPK87_002357"
     CDS             15047..15943
                     /gene="ylqF"
                     /locus_tag="KPK87_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG2538502.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            16332..17624
                     /gene="brnQ"
                     /locus_tag="KPK87_002358"
     CDS             16332..17624
                     /gene="brnQ"
                     /locus_tag="KPK87_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2538503.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7552 a         1648 c         3305 g         5194 t
ORIGIN      
        1 gttaatttat atatgtatgg gaagaaattt tgattacaat gaaaaaactt acaaaagata
       61 tttacgaaaa aacttatagt taaatgttta tatataaaat attatttagg ggttgttttt
      121 attattgaaa ataagaaaga gtggttttat atcaatcgaa tgtataataa gtattgctat
      181 attatatgtg gctgtttatt tagtttctac atcattgtat aattgttata gttttatcag
      241 tagaaatata tctgacagag aaatgttaag tacagcaaaa aaatatatag aagatgagaa
      301 gtatagaata caaaatagta agtatgagtt aattgaagat aagatagaaa aaaattacat
      361 aaatggatat gaaattaaca gtagaataga gcaaatttta gattattatc aatgctatga
      421 aataaatata gagataaaaa atgaatttaa aaaactgagg tttaatagct atgttactag
      481 aaaataaagg ctctatatta ataataacat tgataatatt ttctattata agtactgtgt
      541 gcattatgtg tataggttta atttatacac ataataatat gtttgagtta ggatacaagg
      601 acattcaact aaaagaaatt tctttaagtg gaatagaaat atcagtaagt aatgtacttt
      661 cttgtgttga ggaagctatt gatgtatcta ataatgaaga cgaatttaga gaatatttct
      721 taggaaataa tatgattgaa tgtataaaaa aaataaaaga tacatcttat agtagtttag
      781 aagaactttc tttaaaagta gaaaataata ctatttatga cagaggagat ttttttgaat
      841 ttgatataga aagtaatgtt agagaaaaag aatttttaaa agcaataaaa gtaagagtga
      901 aaatcaaaaa tccatggata ggaattgaca ttgaagatta tatggataaa tctaataaca
      961 ataaaattga tgacaaaaca ttcaataatt ataaagcatc tgaaaataat gaaattctaa
     1021 atcaatttaa aaatgaaagt gaaaatataa ggaatcaaga ggttggtgga tttacagaag
     1081 aagatgaaga atatgaagaa gaacttaaac aaaacataat tgaaaatata aagaagaatt
     1141 ttgatgaaaa aaacctagta caggtatata agtatagggg tgtataactt tgaaaaaaga
     1201 aggaatgtga ctgtaataga attaatagta tctatcagta taatactctt gatagttaca
     1261 ttatcttttc caaaagataa tcttgaaaat cataaaataa atttatttgc acgtcaactt
     1321 tgttcagata taaggtatgt taggcgaatt aatatgttta gcaactacaa tacatatata
     1381 tattatataa atcaaggtaa acacgaaggt tatgtattga gaaaagatgg taaaaacatt
     1441 aaatcaataa tgctacctaa aaatgcaaaa atagctagga gtattgaaat aataaagttt
     1501 aaatctgatg ggtcgcctca aaaggcgtct acaatagaga tatataataa tagattaaga
     1561 aaaactataa caataacacc tgtaagtgga agagtacttc tgaaagaggg aaaatatgaa
     1621 acataaatat ggttacttgc ttttggaaag tgtcgtatca ttgtcttcaa tggtgataat
     1681 tatactagtt ttatattcta tatttttgtc tacaataaac ttaaagttaa aagtagaaga
     1741 taagatagaa ctacaacaac aaagtcttga aatcataaaa tctatggaag gaattattag
     1801 caattctatg ggaataatga atgtatctaa ttatgaagaa acatttaaaa agacaacatc
     1861 tataaaatgt agatatttag atgaaaataa taatgaagaa ggcataagta ataaagaaat
     1921 tatattgaat gaaagacgaa ataagttatt tgtaaattct ttaaatggcg aaagtagtca
     1981 agcaggtggt tatgaaattg gagattatgt agatgagatg tatgtatcaa ttactaataa
     2041 tggtcaatat gttaatatta aattgaaatt atcaaaacgt agtcaaaaat atgagactga
     2101 tttcaaaata aaagtatgga attttagtga aagtatataa aaatacataa atttaagaga
     2161 aagaagtgac atcaataagt catataaaat ataattattg agaactatat aactaaataa
     2221 tatcaatatg taaagtgtat agattaagaa tatagaggat aataataaaa atatagaata
     2281 agtaatttgc caaatacttg tttatattgt ataacttatg atattatgat attagtaaaa
     2341 atttgtaatc ctgacttgac agaattcaag taagagatat aattttaaag gcatagtaat
     2401 gtaaaattta ctaaaaaata agaaatttat tttaatatgg aggaacaaaa aatggtatca
     2461 gcaggtgatt ttagaaaagg tgttacattt gaaaaagatg gacaaccatg tttagtagtt
     2521 gattttcaac acgttaagcc aggtaaagga gctgctttcg ttagaactaa atacaaaaac
     2581 ttaaaaacag gagctataag agaagaaagt ttcaatccaa gtgaaaaatt ccctaaagct
     2641 gttatagata caagacaaat gcaatatcta tacaatgatg gtgagttata ttattttatg
     2701 gatcaagaaa attttgaaca aataccatta aactatgagc aagttgaaga tgcaattaag
     2761 ttcttaaaag aaaatgaagt tgctacaata agattttacc aaggacaacc attccaagtg
     2821 gaagcgccaa actttgcaga gctagaagtt acagatacag agccaggtat aaaaggtgat
     2881 acagcaagta atgtaactaa agcagctaca gttgaaactg gagcagttgt tcaagttcca
     2941 ttatttataa atactggaga taaagtaaag atagatacta gaacaggtga atatttatca
     3001 agagtataaa aatttatgta tataaaagct tatatgtctt aataatataa gatatatgag
     3061 cttttagttt atatataaaa aagaaagcga ggggtgttac atgaaacatt tatatgaaga
     3121 agttgcatac ttaaaaggcc tagcagaagg actagaaata agtgctgaaa gtaaagaagg
     3181 gaaaatgata cataaaatag ttgatgcatt agaagtattt gcagatgcta ttgtaacatt
     3241 ggatgaagaa caagaagaac ttcaagattt tgttgaatct atagatgaag atttagcaga
     3301 tttagaagaa gatattgctg acatggaaga agatctttat gaagaagatg acgatgagga
     3361 tgatgaggac ttcagctata tagaaatgga atgcccaaat tgtggagagt tagtagaaat
     3421 agacgaagat ttgttatatg atgatgaagt agatgttgtt tgtcctgatt gtaaggctgt
     3481 gatattatca tcagaagatg attgtgatga tgattgtaca tgtggtggtt gttcaagttg
     3541 tgatgataga gagtaaaaat tcataagaat taaaactgcc tatgatgcct atgagatatt
     3601 ttgcttcata ggtagtttta tttttatgag gaagggtatt atgtattttg gttttgcaat
     3661 acttggacaa aaatagtaca ctagaattta tagaatagga ttttggaggg atgtaaatat
     3721 gaaaataagt aaaaaattac tagataatat acaaagattt gaaaatgtta taaattataa
     3781 atttaaaaat aaagaatata tactagaagc tcttactcat agttcatatt ctaatgaaaa
     3841 taaaaaatat aattttaacg aaagattgga atttttaggg gattcggttt taggcatagt
     3901 gataagtgat tatttattta atgaagaagc taatctgccc gaaggggaac taactaaact
     3961 tagagcaaat atagtttgtg aagattcatt aagtgaagta gctaatgata taaatttagg
     4021 tattcacatg ttattaggta gaggagaaga agcaacagga ggaagacata ggacttcaat
     4081 attagcagac gcatttgaag ctgtaatagc tgctatatat cttgatggag gttttgaaag
     4141 tgcaagacag tttatacttc atcatatgga aaatataatt tatgattcaa gaaaaggaaa
     4201 tatatttaga gactacaaaa cgcatcttca agaggtgtta caagggaatg gagaaaataa
     4261 tatctggtac aggcttatag aagaaaaagg accagaccat aataaaagat ttgtaatgga
     4321 agttggaatt aatgacgatg tacttggtat aggagaaggc aagagtaaaa aagaagctga
     4381 acagttagct gctaaaatag ctttaaagaa aaaattatgg gaaaaataaa tataatatgt
     4441 aaaaatataa aaagaaatat ttatattaga aggatgttgt agatgaaaaa gagaattata
     4501 cctatatttg ttcctcacaa aggatgtcct catgattgca tattttgtaa tcaaaaaaaa
     4561 ataacaggtg tgagtactga tgttactagt gaagatgcaa gaaatataat tgaggaatgt
     4621 ttagagacta tagataaaga tgcagatgta gaaatagcat tttttggtgg tagctttact
     4681 gctatagata ttgatataca aaaaagttta ttatcagttg ctaaagaata tgtagaaaaa
     4741 ggtcttgtaa aagatattag aatgtctaca agaccagatt gtattgatga agatatatta
     4801 agtatgttaa aagaatataa gacaagtata attgaactag gagtacaatc cttagatgaa
     4861 aaagtattat tagatagtat aagaggtcat caatctgaaa ttgtatacaa gagttcaaag
     4921 atgataaaaa atagtggaat caaactcgga ttacagatga tgattggtct tccagctgac
     4981 acagaagaaa agtgtatatt tacagcaaaa aaatttgtag agctaaaacc agattgtgtt
     5041 agagtctatc caactttggt agtaaaagat acaggtcttg aaaagttaat ggaacaaaat
     5101 aaatataatc catttactct tgaagagagt gtaaatatag taaaaaaagt tcttgtactg
     5161 ttttatgtta ataatatcaa tgtaataaga gttgggcttc aagctactga tgatatacaa
     5221 attggaaaag ctgttttagc aggaccatat catcctgcat ttagagagtt ggtagaagca
     5281 gatatgataa aagattattt agaatttgtt attttgcaaa ataaaaatat aaagcaaatg
     5341 ttggtcaaat ctaataaaaa gaatatttct aaaatcatag gaaataaaaa aacaaatgtt
     5401 aaatatatgg aagaaaaatt tggtgtgctt ttaaaaacac aagaatcaga tttagatata
     5461 aatcaattag aaattgtgtt agatgggaaa agtttaatta gtgctaatat gagagacatt
     5521 catagaaaat tgtatgacat ttatgacctt tagttttaat aatgaaggga gagaaaagat
     5581 ttgtatttaa aaagactaga gttaaaggga tttaaatcct ttccagtaaa aacagatatt
     5641 atttttaaag aaggaataac agccatagta ggaccaaatg ggagtggaaa aagtaatata
     5701 tctgatgctg taagatgggt attaggagaa cagagtatta agagtcttag aggtgataaa
     5761 ctcgaagatg taatatttgc aggtacagat acaaaaaaac ctatgaatta ttgtgaagtg
     5821 gctcttacta ttgataattc tgaaaatcaa ttggaattag attttacaga agtaactata
     5881 agaagaaggg cttatagaaa tggtgaaagt gaattttttc taaataataa aagttgtaga
     5941 ttaaaagata taaaagaagt ctttttagat actggtattg gaaaagatgg ttattcaatc
     6001 atagaacaag gaaaagtaga tgaaatatta agtaataatc ctttaagcag aagaaaagtt
     6061 tttgatgaag cttgtggcat atcaaaatat agatataaaa aacaggaagc agaaaggaat
     6121 ttaagtaata caaaggaaaa tttagaaaga atagatgatg tatatataga aattgaaaat
     6181 caattaaagc ctctttttaa tcaacagaca aaggcaaaaa agtacttgga aataagtgaa
     6241 aaattaaaga cacttgaagt aaatagcttt ataagagaaa ttgaaggaat agaaaaagaa
     6301 ttaagtgagg ttaatgaaca tcgtaaggtt attgaaaagg agctcaatga aaaagaagaa
     6361 caaaaaaatg ttgtagagaa aaagcaagaa gatattaata aagaggtaga ggtattacaa
     6421 gatgtaattg aaaaatctgt agattatata aattcaatta aaggagttat atcaaaaaaa
     6481 gaatcccaaa taaatttaat taaggaaaga ataagaaatt ttacgaatga aattagtaga
     6541 aaaaatttag aaataaagga tataaaagag aagctaaatg aaaataagca atacataaaa
     6601 gaattagaaa gcaataaatt atcaggtagt gaagaactat ctaccttgca ggaaaatata
     6661 aaagtattgg aatcaagtaa agataaacaa aaaattaagt tagaatccct aaataatgag
     6721 atagaattat tgaaagaaag tataattgat atattaaata agaaacaaga gtttagtaat
     6781 aagctgtcca ctttaaacgc aaataaagaa aatatgaata tcagagatga aaatataaat
     6841 tcagagataa cagagttaaa caaaaatatt gaaataaaat catctgaatt agatactata
     6901 aataaagaat ttaatatgca aaatgaaaat ttaaagaatg taaataatag acataaagaa
     6961 ttatcaatta atttgcagga ttcaattagt gaacacaata aactagaaga tgaaatacag
     7021 aaaagtaaat ataatttaaa tggatataat tcaaagttaa atgtctatat agatatggaa
     7081 aatcattatg aaggatttaa tagaggagta aaagaagtac taaaaaataa aaatttaaaa
     7141 ggagttcatg gagcattagg acagattata aatgtacctg aaaagtatga gaaatctata
     7201 gaagcagctc ttggtgctta tatgcaaaat ataataactg ataatgagtt tagtgctaag
     7261 tctgcaataa actatctaaa acagaataat ttaggtagag taacatttct tccattaaat
     7321 ataattaaat caaataaaat aagtctagga aatttaaaag caaatactaa gttcatagga
     7381 atagcaagtg atttaattac atttgatgaa aagtatagaa atataattga aaatatactt
     7441 ggaagaacaa tacttataaa taatatagat gagggcataa aatttgcaaa agaaactggt
     7501 cataggttta aaatagtaac tttagatggt gaaatcttaa atccaggagg ttctttgact
     7561 gggggaagtc taaaaactaa tgggaatata ctatcgagaa aaagatacat taatgaatat
     7621 acagaaaaga taagtaatat caagaatgaa atttcacatc tagaattaaa aagagaatct
     7681 ttagacaaag atgtgaaaaa tataaaaaat gaaattgatt cacatgaaag taaaataaaa
     7741 gatttagaaa aaagtataat aataaaaagt acaagtataa agaatgttga gtctgaaatt
     7801 gaatcgttaa aaggtagtat tactaaatta gaaaatgaga agaatgattt aaattctaac
     7861 ctaaattata cacttgaaaa aagtgatgat gttagaaaag atatggaaga gttagatgat
     7921 ttatacaata aaaataaaga aaaaattgat gcattgaatg aggaaataaa aagatacaat
     7981 gatttatatg ataaagaaaa atctgaattt gatgaattga acttaagttt agtgaagaaa
     8041 acagaagttt acaatagtat agttagagat ataaaaagaa tatctggtga aaattgtgag
     8101 ttagaagaaa aaaataaaca attagaagag tcattaaatt atgaagaaca tgaaataatt
     8161 aaattacaag attctatact cactgaagaa aaagaaaaag aaaatttaac aaaacaacta
     8221 ggggatagta atagaaatct tgaaactaga aaaatagcaa aagatgattt aaaaaatagt
     8281 tttgatgaaa ttaataaaga attaaaaact atagatagac agcatataga acttaaagaa
     8341 agtttattta aagttggtgg aagattagaa agattaaaaa ctagtcaaga cacatatata
     8401 aataagttgt ttgagcaata tgatatgacc ttagtacaag cattggaaat taaagatgaa
     8461 gatttggata tagatagaaa attcttagaa agtctaaaga gagaaataag aagtcttgga
     8521 aatataaata tagactctat aaaggaatat gaggaaataa aagaaagata cgatttttat
     8581 agcgaacaaa agcaagattt agaagagtct atggaagaga tagaaaaact tatacatact
     8641 ttagaagaaa atatgaaatc tgaatttgaa attaagtttg aagaaataag taaaaatttt
     8701 aaatatgtat ataagagatt atttggtgga ggttgtggag aattaactat tttagataaa
     8761 gaaaatttat tagaaagtga tatactaata acagctcaac ccccaggtaa aaaaatgaaa
     8821 aatttaaacc tattatctgg tggagagaaa gctttaacgg caataagtat attatttgct
     8881 atattgatta caaagccaac accattttgt atattagatg agattgaagc gccacttgat
     8941 gacgcaaata tatttagatt tggagaattt ttgaaggatt tatctaaaga gactcaattt
     9001 atatcagtaa ctcataggag aggaacaatg gaagctgctg attatatcta cggagttaca
     9061 atgcaagaaa aagccatttc aaaagttatt agtttaaaat taaaagaagc tcaggaaata
     9121 acagatatta tataacttag attgacatag gaggaataac agtgttaaaa aaattatttg
     9181 gttttggaaa agacaaggaa aaggaaatag aaaaaaaaga tgctgaagaa gaaatagaag
     9241 ttgaagacag tgttgataat ttagaaaact tggaagaaac tattttttca ggacttgaag
     9301 aagaagtgat agataaagtg gaagatgttg aagaaaaaaa tgaggaaagt gtcaataatg
     9361 aaggcactga agaaatcgaa aactttgaaa aaatagaagt tgagatgaat agtaatggaa
     9421 aagaagtaga aaatatttca aataatgatt ctcaagaaga gataatcgaa gaattagaaa
     9481 attatgatga aattgagtct aaagagatag aagataaaga agataagaaa gttaacttgt
     9541 ttgaaagact aaaacaagga ttgacaaaag caaagcaagg aataactgat agaatagatg
     9601 aagttttaaa atcatataca aagatagatg aagaattact tgaagattta gaggaaattt
     9661 taataacagc agatgttggt gtaaatacaa ctatggatat aattgaaaga ctaagagata
     9721 aaataaagca aaaaggtatt actgaaccaa taaaggtaag agaagaactt aaatctatag
     9781 ttgaagatat tcttacaaat gaaaactcta cactagatat agaaccagca ccatgtataa
     9841 tattaatggt gggtgtaaat ggggtcggta agactactac aataggtaaa ctagcaaata
     9901 gatacaaaaa agatggtaaa aaagttttat tagcagcagc agatacattt agagcagcag
     9961 cgacagagca attagaaatt tgggcaaata gaaccaatgt agacataata aaacatcaag
    10021 agggagcaga ccctggagct gtagtatttg atgctataaa agctgcaaaa gctagaaaaa
    10081 cagatgtatt aatatgtgat acagcaggaa gattacacaa taaggctaat cttatgaatg
    10141 aacttggaaa agtatttaaa atagtagata gagaatttcc agaagcaaaa cgtgaagtac
    10201 ttcttgtggt ggatgctaca acaggtcaaa atgcagttgt tcaagcaaag acatttaaag
    10261 aagttgcaga tataacaggt atagtactta caaaacttga tggaactgca aaaggtggag
    10321 tagtccttgc ggtaaaatca gaagtagatg tacctgtaaa acttattgga gttggagaaa
    10381 gtgtagaaga cctacaagat ttcaatgcaa aatcattttc agatgcatta tttggtaact
    10441 aatgttcttt taaaaataat ggttgacaag agtgttgtta tgatataaaa tacaatagtg
    10501 taaagtaaat aacctttaca gtatactgct aggaagtgta agatatgaat atagaaaaaa
    10561 tggtcgaaat tggattgtta tttgaacagt ataaagaatt attaactgac aagcagaaag
    10621 aaatagttgc tctatactat gaagaagatt attctttagg tgaaattagt gaaaatctga
    10681 atgtatcaag acaaggtgtt tatgatacct taaagcgttc agaaaaaata ctaagagatt
    10741 atgaagaaaa gttacactta gtatcaaaga ttcaagagca ggaaaaaaat attaagatta
    10801 taaaagataa aattattgac attaaagaag atttattgca caatagagat tgtgctaatt
    10861 taatccctaa gctagaaaat atagaagatg tatgtaggga gatgataaaa tgatatttga
    10921 aggattgtct gataaactac aaggggcatt tagtaagtta aaatctaaag gaaaacttac
    10981 agaagtagat gtaaaaaatg ctatgagaga agttaaactt gctcttcttg aggcagatgt
    11041 taactttaaa gtagttaaag atttcataaa aaaagttcaa gagagatgtg ttgggcaaga
    11101 agttatgcaa agcttaactc cagctcagca tgtaatcaaa attgtaaatg aagagcttac
    11161 aagtttaatg ggagatgtac aaagtaaagt aatgatttcc tcaaagcctc ctacaatttt
    11221 aatgatggtt ggattgcaag gtgcaggtaa gactactaca gcaggaaaac ttggaggata
    11281 ttttaaaaag caaggtaaaa aacctctatt agtagcttgt gatatttata gaccagcagc
    11341 tataaagcaa cttcaagttg ttggagaaaa attggatata cctgtattca atatgggaga
    11401 taaagaaaat ccagtaaata ttgcgaaagc aggtttaagc catgcgatta aaaatgctaa
    11461 tgatttagtt attatagata cagctggtag acttcatata gatgaagtac ttatggatga
    11521 gttaaagtct attaaatcag aagttaagcc acatgagata cttttggttg tagattctat
    11581 gacaggtcaa gatgctgtaa atgttgcaga aagcttcaat gaggcacttg gtgttgatgg
    11641 agtagtatta actaaattgg atggagatac tagaggtggt gctgcacttt caataagagc
    11701 agttacacaa aagcctataa aatttatggg gatgggagaa aagctagatg atatagaacc
    11761 tttccaccca gatagaatgg catcaagaat attaggtatg ggagatgtct taagtttaat
    11821 agaaaaagca caagaaaata tagacttaga gaaagctaaa gaattagagc aaaaaattaa
    11881 aaaacaagag cttgattttg aagatttctt acaacaaatg gaacaaatac aaaatatggg
    11941 acctcttgac aagatacttg gtatgatacc tggtatggga aatgtaaaag accaacttgg
    12001 agatgtagat ttgaatggca aagaaatgaa gagaacaaaa gccataattc agtcaatgac
    12061 tacatatgag agaagaaacc caggagtatt aaatgcttca agaaagaaaa gaatagctaa
    12121 aggcagtgga actagtgtac aagatgtaaa tagacttata aaacaactta atgaaatgaa
    12181 aaaaatgatg aagatgtttg ctggttcaca aaagagtatg aagaaaaggg gcggcttagg
    12241 cggtttacct ttttttaaat aaaatataaa aaagtaaaaa ttattcggag gtgactgtaa
    12301 tggcagttaa aataagatta aaaagaatgg gagctaacaa aaaacctttc tacagaatag
    12361 tagtagctga ttcaagagct ccaagagatg gaaaatttat agaagaaata ggatactata
    12421 atccaatttc tgaacctaaa caagtaagaa taaatgatga aaaagctata aaatggttag
    12481 ctactggtgc tcagccaaca gaagtagtta aaaaattact tgtaaaaaac ggagtaatag
    12541 aaaaattcga agcttctaaa caagcaaaat aattttttct tggcaattag tttaagtaag
    12601 ggctcattgc taaggttact ttaggagatg aaatatatga aagagctagt tgtagatata
    12661 gctaaggctc tagttgataa tcctgactca gttgttgttg aggaatttga agataatgat
    12721 ggtatcatct taaagcttac agttgctcaa gaggatatgg gtaaggttat tgggaagcag
    12781 ggaagaatag ctaaagctat aagaactgtc gtaagatctg ttgcaaatag agaaaatata
    12841 aaagtttctc ttgagatagt ataaaatagg aggattaggt ataatgccta gtccttttta
    12901 tataaatgcg tagtttaaga ttttatgatg atatgaccac ataagattaa attttttaag
    12961 aaggtgataa catgaaagaa aaattaactc attttaaggt aggtcaaata gtaaatacac
    13021 aaggtttaaa aggtgaagta agagtttatc cgcttactga tgatatagag aggtttgatg
    13081 aattaaaaga tttctatctg ggaaaagatt taaataataa attggctgtt gaaaaagtaa
    13141 gatacaaagg ttctatggtt ataatgaaga taaaaggcat agacagcata gagaaagcag
    13201 aaaaactaaa aaataaattt atgtatgtat ctagagaaga tagcagagag cttgaagaag
    13261 gcgaattttt tatagcagat atgattggaa tggaagtttt aactgtagat ggaaaatatg
    13321 tagggatact agaagatgtt cttcaatact cagcaaatga tgtctatgta gtaaaaggtg
    13381 aggaagataa agaatttatg ataccagcta taaaaaaatt tgtacctaca atagatattg
    13441 atgaaagaaa aatgattata gaccctatta agggaatgat agattaggtg gtaatatgag
    13501 atttcatata atgacacttt ttccagaaat atttaattcg tacatggatg aaagtataat
    13561 gaagagagca gttgaaaaag gtataattga ggttcatata tataatataa gagatttttc
    13621 taataataaa cataaaaaag tagatgatta tccatttgga ggaggagcgg ggatggtaat
    13681 gactcctcaa cctatatatg acacatataa gtatataata acaactcata atataaataa
    13741 cccaagtgta atttatctta ctccaaaagg taaggtatac aatcaatcta tggcaaagca
    13801 gatgtcttta aaagaagata taatactact atgtggtcat tatgaaggaa tagatgagag
    13861 aatcattgat ttaatagtta ctgatgaaat ttcaattgga gattatatat taactggggg
    13921 agagctccca gcacttataa tgatagattc tatatcgaga ttaataccag gtgtattaaa
    13981 ccaagaagaa tcatttgaag aagaatcatt taaagacaac ttattggaat atcctcatta
    14041 cactagacct agagattttg aaggattaaa agtaccagaa gtgcttttat cagggaatca
    14101 taagaagata gatgagtgga gaagagaaga atctataagg ataaccaaag aaagacgatt
    14161 tgatttatat aaaaagtcta atgaaaaata aaaatataat gaaaaattaa tatatatgta
    14221 tacaataaag tacctataaa tggaattaaa aacataaaag tgcttgtaat aactattgaa
    14281 gtatagtata atataaaatg ttgaaaatac gggcgttcct ctgtttacac tggtcgggta
    14341 gttaagaacg tctatgatat ctaggaggaa gaaagatgaa tgaaatgcta agagcaattg
    14401 agcaagaaca attaaaaaat gaagttccta attttggacc tggggacaca gttaaagtac
    14461 acgttagaat tatagaagga aaaagagaaa gaatacaagt attcgaaggt gtagtattaa
    14521 agagacaagg tggaggagca agagaaactt tcacagttag aaaaatgtct ttcaatgttg
    14581 gtgtagaaag aactttccca gttcactctc caaaaataga aaaaatagaa gttactagaa
    14641 aaggtaaagt aagaagagct aaactaaact acttaagagg tagagtaggt aaagctgcta
    14701 agataaaaga agctagaaat aaataattaa aaactttact tataaatggg gggctgattt
    14761 atcagtcctc tttctatttt aatgaaaatt acagcaaata attatactag aaataaatag
    14821 aaatatttgt tgaaaatata cattatttat ctatgaattt atagctttta aaatgaaaaa
    14881 taaaattggt aaatattgca ttatattttg taacgatatt aaaaataaga gaacagtatt
    14941 atgtaaagta tgacgttaaa aagatgacaa tattatagtt tgttaagata gattttttaa
    15001 gtggaattag gatataataa tataatatta ataaggagtg atacatgtgt caaatgaata
    15061 cgaagagtat cttatgaatg acaatctaca tataaactgg tatccaggtc atatgaaaaa
    15121 aactaaggag ttagtaaaaa ataacttaaa attgattgat gtagtaattg aacttttgga
    15181 tgctagaatc ccgtttagta gtaaaaatcc agacatagac aggcttgtag gagataagcc
    15241 aagagttgtc gtacttaata agagtgatat ggcagatagg gataaattga atcaatggat
    15301 tgaatactat aagaaaatta atatcaaagc tataccagta gatacaataa aaggcgttgg
    15361 tataaataag ataatcgaag aatgtaaaaa tgtaactaga gaaaaaatga gttctttaaa
    15421 agacaaaggg agaaaagaaa gagctatacg aataatgata gttggagttc caaacgttgg
    15481 taaatcgtct cttataaaca aattaacagg tagaaaaagc actcaaactg gagataagcc
    15541 aggagtaacg aaagggaaac aatgggttag attaaaggga aatcttgaac ttttagatac
    15601 acctggaata ctttggccta agtttgaaga ccaagaagtt gcattaaatt tagcatttag
    15661 tagagctata aaggatgaga ttttagatac tgaaactttg gctttaagac ttatagaaaa
    15721 acttatgaaa atagaacctg agaagttgaa agccagatat aagttagatt gtctaggaga
    15781 aactccaatt gaaactatgg atatgattgg ccataaaaga gggtttataa caggtaaaaa
    15841 agaactagat tatacacgca tagccacaac tgtattaaat gaatttagag atggaaaaat
    15901 aggaaatata acattggaag ttccagaaaa cgttaaaaga tagattttaa aacaaaatca
    15961 aaaatttaat gttaaaaatt taaaaaagcc ttttagcaaa ggctttttta tttttttgaa
    16021 atatagacaa atatctccat ataaaagtat aaattaggtt agaaaacctt taaaaatatt
    16081 aatttaagac aaatatacct aatattttga aaattaaaat gagtgtgatt atttaaaatt
    16141 ttagcatatt gacttgcttg tttcgtataa tatataataa gcacaaataa atacaatata
    16201 ttaaaaattt aatttattta attttattaa tttaataata agttcttatt gtttaattca
    16261 acttacttat gcaagcgtaa acaatcgaga cattttatta atttaataag gttaaaaagg
    16321 gggattttat aatgggtaag accaaggatg ttatagtatt tggatttgcg ttatttgcaa
    16381 tgttctttgg agcaggtaac ttaatatttc caccttactt gggaataatt acaggtccag
    16441 aatggttaat tgccttctta ggatttacgt ttgctgatgc tggattggca cttctagcag
    16501 taatggctac agctaaattc gatggaaacg tagttgaaat gtttaaaaga tgtggtatga
    16561 agttagggat tcttataggt tgtgcagata ttttatgtat aggaccattc ttagctatac
    16621 caagaacagg tgcaacaact tatgaaatgg gtataatgcc tttatttgga acttcaattc
    16681 cagtgttatt attctgtata cttttttttg cgatttcata tgtactaaca ataagacctt
    16741 ctaaggtcgt agatatagtt ggacagtttt taactccagc tcttctaata gctttagcat
    16801 ttataattat aaaaggtatt atatcacctc ttggagatat agtagataaa cctatgatac
    16861 ctaatgtatt tgcagaaggt ataggtcaag gatatcagac aatggatgct tttgcagcaa
    16921 tagcacttgc atcagtgcta atagtatctt taaatgataa aggatattct actattagtg
    16981 acaagttgaa aatgattggt aaggcgggtg ttcttgcttg tggaggatta gctctagttt
    17041 atggaggatt atgtttcctt ggggcaactg tatcaacaat gtatggaaca gatgcagtac
    17101 aatcacaagt aatagtaaat ataactgaag gattgcttgg aaatgttggg aaagcgattt
    17161 tagcagtagt agtatcatta gcatgtttaa ctacatctat aggtcttaca tctgccacag
    17221 gtcaatattt ctctagactt acaaaaggta aattgagtta tgaaaaaata gtattagctg
    17281 tatctgtatt cagtgcagta gtagcatctt ttggtgttgg aactataata aagatagcat
    17341 ctcctatact aagtatagtt tatccaccat caattgtttt aataatttta gctttcttca
    17401 atgaaaaaat taagaatgat aatgtataca aaggagcagt atatatgtca ttgcttgtaa
    17461 gtatactaac agttatatct agttatggag tagctgttcc agtagtaaat tctttacctc
    17521 ttaattcatt aggatttaac tgggtggtgc cagttataat agctggaata attggtaatt
    17581 ttataccatc aaagagtcaa tctaatacac ttggaacaaa ctaagttttg tttaattaaa
    17641 atataatcga ttgtaaataa atgagttaac taaaagtaaa aggagttatt tgtacaaat
//
LOCUS       DAEMIK010000085        17501 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEMIK010000085 DAEMIK010000000
VERSION     DAEMIK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.085"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..3654
                     /locus_tag="KPK87_002359"
     CDS             106..3654
                     /locus_tag="KPK87_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2538504.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3839..5773
                     /locus_tag="KPK87_002360"
     CDS             3839..5773
                     /locus_tag="KPK87_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG2538505.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5879..6874
                     /locus_tag="KPK87_002361"
     CDS             5879..6874
                     /locus_tag="KPK87_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2538506.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6846..7631
                     /locus_tag="KPK87_002362"
     CDS             6846..7631
                     /locus_tag="KPK87_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2538507.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7619..8317
                     /locus_tag="KPK87_002363"
     CDS             7619..8317
                     /locus_tag="KPK87_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538508.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8440..8913
                     /gene="ybaK"
                     /locus_tag="KPK87_002364"
     CDS             8440..8913
                     /gene="ybaK"
                     /locus_tag="KPK87_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG2538509.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9110..9949
                     /locus_tag="KPK87_002365"
     CDS             9110..9949
                     /locus_tag="KPK87_002365"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG2538510.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            9962..10510
                     /locus_tag="KPK87_002366"
     CDS             9962..10510
                     /locus_tag="KPK87_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG2538511.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10576..11757
                     /locus_tag="KPK87_002367"
     CDS             10576..11757
                     /locus_tag="KPK87_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG2538512.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12052..12438
                     /locus_tag="KPK87_002368"
     CDS             12052..12438
                     /locus_tag="KPK87_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2538513.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYNMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12588..12851
                     /locus_tag="KPK87_002369"
     CDS             12588..12851
                     /locus_tag="KPK87_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2538514.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(12980..13822)
                     /locus_tag="KPK87_002370"
     CDS             complement(12980..13822)
                     /locus_tag="KPK87_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2538515.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14117..14851
                     /locus_tag="KPK87_002371"
     CDS             14117..14851
                     /locus_tag="KPK87_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2538516.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14861..15994
                     /gene="nagA"
                     /locus_tag="KPK87_002372"
     CDS             14861..15994
                     /gene="nagA"
                     /locus_tag="KPK87_002372"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG2538517.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAKVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            16093..16842
                     /gene="nagB"
                     /locus_tag="KPK87_002373"
     CDS             16093..16842
                     /gene="nagB"
                     /locus_tag="KPK87_002373"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG2538518.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17017..17289
                     /locus_tag="KPK87_002374"
     CDS             17017..17289
                     /locus_tag="KPK87_002374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538519.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         6989 a         1856 c         3104 g         5552 t
ORIGIN      
        1 ggtaaatgta tattgaaaaa atcatagaaa tagaagtaaa caatttaaat aaatacaaag
       61 gtttgcaaaa gaagtaatat aattatatac aatgaggatg tgagtatgat gaacagacta
      121 aagccaagaa ttttaggtat tagtatcttg tttattatta taattatttt aacatgtgga
      181 gctgttttca agtttaaatt taaattagat aataaattaa aagaaaccac acatacatat
      241 ttgtctgagt ttacagaaca caatataatt aatctcaaga caagacttag tggtcaattt
      301 gacatgcttg aatctattgc taacttcata ggaaacttga ataatataaa tgatgaaatg
      361 gtgattgatt taatggattc tgatgtaaag cgaagttctt taattagaat gactatctct
      421 acattagatg gggaatgtta ttctaatgat ggaattaccc ataatgttca agatagagat
      481 tattttaaaa aagctaaaaa tggagaaaag aatatatcag agcctttaaa atctattgtt
      541 aataataaag aaataatagt tttatctacc ccagtttata aaagtgggaa aatagttggt
      601 gttttaagtg gaatttatga ttcaaaaaag ttaaatgagt tattaggatt atctgcattt
      661 gatgaaaaag cagatgtttt tgttgcaaag aaaaatggag atatagttgc tcaaaaaaca
      721 aataaaaata gaaagacgct atcatgtata tttcagcttt ttgacaaaga taattgtgat
      781 gaagatgatt gtctaaaaac tataaaagaa aatatgaaaa atgataagtc tggatatttt
      841 aattataact tagataatga aaaatttatg ttaaactatc agccacttgg agttaatgat
      901 tggtatgttt tttctgttgt gccaaataaa atagtatcta ttcagtcaga gaatatatct
      961 tatgatgtat atatttttgc attagaaata ctactagtta tattgatttt tataatatat
     1021 atagcatgta ttataaataa taacaccaaa attattataa atgaaaaaga aaaatttaaa
     1081 gtactcactg ataatatatt aggaggggtt aaaacctgtt taaaagatga atatatgact
     1141 atgacatatg taagtcaggg gtttttagag cttacaggtt actcttgtga agatttaaat
     1201 aaaatattta aaaatagatt taaagacatg atttataaag atgatttaga caagtttaat
     1261 tgtattattc taaatcaatt agaaaaacaa gataaaattg aagttgaata tagaattgta
     1321 agaaaagatg gaagtataat ttggattttg gagagaagta agtctataaa tgataataaa
     1381 ggaagtgagt gtattcaggg agttttaact gatataacag aacttaagaa aatacaagaa
     1441 gaattagatt caaaaagatt tgaaatacag gctatcaatg atagtattgt tggtggaata
     1501 gtgataactg agataaatga tgattttgat agtatatatt taaatgaagg ttatttaaat
     1561 atgattggat atacacgcaa acaactggaa gatgagctaa ataataaatt aatcaaactt
     1621 atttttaaag atgatagaac agaattaatt aatagtataa acaaacaact caaattaagt
     1681 gatacatttg catctgaaca aagagttgaa aaaagggatg gcactattat atggatttta
     1741 ttaaaaggaa gaaagatgat ttatgataat ggaaaatctg tactttgttg tgttattgta
     1801 gatattactt ctgctaaatt attagagcaa gaacttaaaa agagtgaaga acgtcatgaa
     1861 atcattctaa agcagacgca aaatattata atagaatggg atatagaaaa agataaaatg
     1921 aatatatcac ctaattggta taataagttt aaatatgacc ctataactga aaacgcaaca
     1981 aaaaatataa ttaaatcagt agatatatat catgatgata tggataagtt tatttcatta
     2041 attaaactta cttcacaagg tagtgagtat gaagagtgtg aaattagact tagaactaga
     2101 gatactattt acgcttggtt tagtataaag gcaacaactt tatttgataa taatggtaaa
     2161 ccttataaag tagtaggaag tttgactgat attgatgaat caaaacgaga agctgaaaag
     2221 ttgaaagaaa gggcagaaaa agaccctctt acagatctgt ataataaaaa agcatcacaa
     2281 tcacttatag aggagtacat aacattggat aaaaaaagaa aaggtgcttt aatgattatt
     2341 gatattgatg actttaaagg aataaatgat aatttaggac atttatatgg ggatgctgta
     2401 ttaagtgaaa ttgcaaatga cctaataaat ctatttagag caaccgatat agtaggcaga
     2461 attggtggag atgagtttat tgtatttata aaagatattt ctgaaacaag agatattata
     2521 aataaagcag aagaattggt aaaaacattt gaaaggtctt ttttaggtaa aggacgaaat
     2581 tataaaatat cattaagtgt aggaattgct aagtttccac aagatggtat ggactatatg
     2641 acactattta gaaatgcgga tatagctctt tactcagcca aagggaaggg caagaattgt
     2701 tatacattgt atgataaaaa atttgataaa gtccaatata taactaatac aagaaatgca
     2761 ggagatagtg ataatgtaga gatgtctttt aaagaacata tcacaaatta tatatttgat
     2821 atactatatg aatcaagtga tatagaagtg gcagtaaatt taattctaag tattgtaggt
     2881 aaacacttta atgtgagtcg catgtatata tttgaaaatt cagatgataa tcgagtatgt
     2941 aaaaatacat ttgaatggtg taacgaaggt gtaacctcgg aaattaataa tcttaaaaat
     3001 atttgttaca aggatttagg caactatctt ttaaatttta atgcagatgg gttattttat
     3061 tgtaatgata ttgaaaattt aggtagtgat gtgaaaaaag tacttaagcc acaaggcata
     3121 cgttcaatat tacaatgtaa aattttagag ggtaatgaat ttagagggtt cataggattt
     3181 gatgaatgtt catataatcg tatgtggact aatgatgaag tgaacacttt gacatttata
     3241 tctaaaacgc tcagtatgtt tttattaaaa atgcgtattc agagtaagct agaagagtct
     3301 ttaacaattc aaaaatctgt gatggataat atggatatat gggcatatgt tgttgacagg
     3361 aatacctatg aattactatt tatcaataaa aaaatgcttg agtcagcacc aaacactcat
     3421 gtaggtgatt tatgttataa agcgatatgg aataatcaag ataaaccttg taaaaaatgc
     3481 cctatgatat atttaaaaga tggtgatgat acttataaat cattcatgca taataaaaag
     3541 tttgaggtac aggcaaatat aactacaact actttgaaat ggtttgatgg taaagatgca
     3601 tgtttaatat ttggaagtga aataactagt attgaagctg aagaaaatac ttaaaatact
     3661 gtgttatata tatacttaaa atcgcaattt gaatttatta gacttaaaat gtctatataa
     3721 atcatttaag ttgtggtttt aagtattttt tatgatataa ttaagcgtta agaacaaaat
     3781 taaaagatac ttttgtataa aaaaatattt gttaaatata tattgagcag gtgataatat
     3841 ggaaaataag tttttaccaa taagcaaaca agatatgata gatagaggat gggaagaatt
     3901 agattttgtt ctagttacag gagatgctta tgtggaccat catagttttg gaacagcaat
     3961 aatatctaga gttttagaaa gtgctgggta taaagttgga ataatagctc aaccagactg
     4021 gagaactaca gatgatttta tgaagctagg aaaacctaga cttgcatttc tagttaatgc
     4081 aggaaatatg gattcaatgg tcaatcatta ttctgtaagt aaaaagcata gagaaaagga
     4141 tatgtattct ccaggtggga aaatgggtta tagaccagat agagctacta tagtatattg
     4201 caataaaata agagaagcat acagtgatgt tgcaatagta ataggtggaa ttgaagcgag
     4261 cttaagaaga tttgcacact atgattattg gagtgataag gttagaaagt ctatgttaat
     4321 agatagtgga gcagatttat tagtttatgg tatgagtgaa aagcagattg tagaagtagc
     4381 caatgcactt aatgatggat atgacccaaa atacattaga catattgatg ggacatgcta
     4441 tatttcagat acattggaag aaatatatga taaatatata ttaattccat catataaaga
     4501 aatttgtgaa gataaaatga agtatgtaga agcttttaaa attcaatatg atgagcaaga
     4561 cccatttaga ggtaatataa ttgttcaacc tcatggaagt aagtatctgg ttcaaaataa
     4621 gccagaaaaa ccacttagta gagaagaact agatgaagta tatggattac catatcagaa
     4681 gacataccat cctgtgtatg aaaaatttgg aggaatacca gctatagaag aagtaaaatt
     4741 tagtatagta agttcaagag gctgttttgg tagttgttca ttttgtgcaa taacatttca
     4801 tcaaggtaga gcagtgcaaa gtagaagtga aaaatcaatt attgatgaag caataggaat
     4861 aacaaattta gatgatttta aagggtatat acatgatgtt ggaggtccaa cagctaattt
     4921 tagaagacca gcatgtaaaa aacaaattac taaaggtgct tgtaaaaata gacaatgctt
     4981 atctccatca ccatgtaaaa atttagatgc agaccacagt gaatacttac atttacttag
     5041 agctgtaaga aaattaccta aaataaaaaa ggtgtttgtg cgttctggaa ttagatacga
     5101 ttatgtaatg gcagataaga acaataaatt tcttagagag ttgatagagc atcatgtaag
     5161 tggtcagtta aaggtagccc cagaacatat atcagaagaa gttttaaagt atatgcaaaa
     5221 accagcagga aaaacttatg ataaatttag acagaagttt tttgctataa atgaagaatt
     5281 aggtaaaaag caatatttga taccctattt aatgtctagt caccctggtt ctaccttaaa
     5341 tagcgctata gagttagcag agtatttaag agatactcac taccaaccag agcaggttca
     5401 ggatttttat ccaactccag ggactctatc tacaaccatg ttttatacag gtattgaccc
     5461 gcttacaatg aagccagttt atgttcctaa atcaaaacgt gacaaggcga tgcaaagggc
     5521 tttattacaa tatagagcac ctagaaatta tgatttggta tactctgcgt tagttgaagc
     5581 aggaagagaa gacttaattg gttttggaca tagatgtctt ataaagccaa aaaatgaaaa
     5641 gccttatttt aatagaaata attctaaaaa aaatgtatct aaaggaacaa ataaaaacaa
     5701 aaaaactaat acaaataaca gaagtaataa aaatcaacaa aaaagttcaa ctaaaaaaaa
     5761 gaaaaaacat taaagtataa ataaaaatac ttaaaaaaga aggattattc gataaattaa
     5821 cagaaattag tataaataaa tataattatt ttaatgacgt tacctttggg aggaagagat
     5881 gaaaaagtat atttctataa tattattagt ggtgttgact atggttgttt tggtaggatg
     5941 ttcaccagga aaagataacc ctaaaaataa ggagctatca gttgtcaaac aagtaaaagt
     6001 agctgttcca gatggactac cagcagtggc aatagctaaa cttgcaaatg aaaatccaga
     6061 gattaaagaa ggatatgaaa cagtgtatag cattgaaaag acacctgaag cgatttccac
     6121 tagagtgatg aaaaaagatg cagatatagc tatagttcct tcaaacatgg ctgcaatagc
     6181 gtataataaa acttcatcat ataatattgt tggaactgta ggaatgggtt cactttattt
     6241 ggtgtcaaca gaaaatataa aagattattc tgatttagtt ggtaaagaag ttggatgtac
     6301 tggaaaagga cttactccag acataactat aaaaagtctt ttgttacaaa aggatataaa
     6361 ttattcagat ataaaattta attatgtaaa ttcagcaagt gaactggtac cattattggc
     6421 tactggtaaa gttaaaacag gtattgttcc agaacctgca cttagtgctt tgatgtctaa
     6481 aaatccagac ataaaaatta taaaaagttt aaatgactca tggaaggaag taagtggttc
     6541 aaaagatggt tatcctcaat ctactttaat tgtaaagtct gaatttttaa gagataataa
     6601 agattttgtt gattcttttg taggtcaatt atcaaatagt atagattggg caaacaaaaa
     6661 tcctgaagaa ttaggtgcat attctgaaaa aataaaaata tctactgaat caaagataat
     6721 aggaaaatct cttgaaagag ctaatttaaa atatattcca gttaaggaca tgattaaaga
     6781 ttataaaaat tactatgaga aattagctaa ttttgatgat aagacattag gaggcaaagt
     6841 accagatgaa gcaatatact ttgttgagaa ataaaaaaaa taaagaacaa tttatagata
     6901 aaatggaggt aacactttct tgtgttatcc tcttattttt atggcaaatt attgctttaa
     6961 aaattgataa tgatattttt ttaccaacag taggtcaagt atttaatagt ataaaagaga
     7021 ttatgctaaa ttctagtttc tatatagata tactttactc tatggggaga tgttttttta
     7081 gttttttact ggctatgatt tttgctatta tactttcaat agtgtcttat ttaaatagat
     7141 tttttagaaa cttattaaag ccaatcaatg ctttgacacg ttcaatacca actatgatat
     7201 tggtagtact tgcacttatt tggtttgaaa aggatagtac cccttttata gttggattta
     7261 ctatagtttt tcctatattg tatgataacg ttttgggcgc tatattgaat atagacaaga
     7321 acttattaga aatggctaat gtgtataaaa taaggtttat agataaggtg ttaaaaattt
     7381 atttaccagc cataaaattt caaattatat ctatactagt atcaacattc tctttaagtt
     7441 tgaaagttgc tatagcagga gaggtatata gtcaacctac atatggtatt ggaactatga
     7501 tacagactga aaaaattaac tttaacacat ctggaatatt tgcatggata attattgttc
     7561 ttttaatatc tgctatttta caaatagctc aaaagttttt agcaaggaga gcttttttat
     7621 ggaaaagata aagttgagta ttgaaaatat aaataaaaga tatgatagtc gtattatatt
     7681 tagagatttt aatattgatt tttatgtaaa tgaggtaaat tgtatccttg gaaaatctgg
     7741 ttgtggaaaa actacactct tgaacataat aagtgggatt attaaaaatg acaccaacaa
     7801 tctaaatatc aaagaaaatt taaatagagt aggaaataag cttgaagcta gttacatatt
     7861 tcaagatgat agacttatag actggttaac agtagaagaa aatataaaga tagttgtaaa
     7921 taaatactat aataaaactc aattaaataa aatttgtgat gaatacttgg aattggttgg
     7981 aattagtgat tataaaaaat tctatcctca gatgcttagt ggaggtatta gacaaagagt
     8041 aaatattgct agagctttta tatatccatc aaaaaatatt ataatggatg aaccttttaa
     8101 atctattgat gcaaagaata cacagcttat aatggataat ttcagaaata tattaagaaa
     8161 agaaaaaaga actgtgttat ttgtaactca taatattgag gaagcattgt ttttagcaga
     8221 taggatattt attttaggag actctccaat tagaataaaa aaaatattaa aaaattctaa
     8281 ggaattggaa aaaaatgaag ttttgaagtt gatatagagt tataataata ctgtcgtttt
     8341 aaaatatatt tggttaaaat ctataagact atattaaaat agtacagaat attcaaaact
     8401 gtgtataatg ttcgtatttt ataaaaagga agtgaatgta tggttaagac taatgctatg
     8461 agaatattag attcaaataa gattgattat aaagtaatgt cttatgaagt gaaaagtgaa
     8521 catgtggatg gtgttgaggt ggcacatgat ataggaagag atgtaaatga agtgtataag
     8581 acattagtaa ctcaaggagt tagtaagaat atatatgtat atgtaatacc agtacatgaa
     8641 aacttagact taaaaaaggc tgcaaaagta gctaaagaga aaagtgtaga gatgattcat
     8701 gtaaaagata taaataagct tacaggttat attagaggcg gatgttctcc tataggtatg
     8761 aaaaagcttt ataaaacttt tgtaaatgaa agtgcaaaga atttagatac aattattgtg
     8821 agtgcaggaa aaataggata tcaaatagaa ctatctccat ttgatttaca aagacttata
     8881 aaagtagaat ttgtagatgt aataaaaaaa taagaattac atataaaata tagagcttat
     8941 attcttaata ataggtgttg tagtattaat aaaatgaata ataattttaa aataaatatt
     9001 cagttactta tgtgtaattg gtataaaaat attgagaaat tttaaaaaca atggtaaagt
     9061 gtaagtataa tcacgaatat tttttataaa gtaatggagg gatgttcgct tgagaaaaat
     9121 aaaatctgaa caaatcgtcg agcaggtaaa aaaactttgt atagaagcga gcctatattt
     9181 aggagaagat gttttaagct gtataaaaga aaaagcgaaa agtgaaaaga gtgaagttgg
     9241 aaaaaatata ttgaatatat tagttgaaaa tgctgaaata gctaaagaaa aaaatatacc
     9301 aatatgccaa gatactggta tggctgtatt ttttgttgaa ataggacaag aagttttaat
     9361 tgaaggagac acacttactg atgctataaa tgagggagta agacaagggt atgaagaagg
     9421 ctacttaaga aaatctgttg taagtccaat taacagagta aatacaaaag ataatacacc
     9481 tgctgttatt cattatgata tggtaaaagg tgataaaatt aaaatagaat ttgcagctaa
     9541 gggatttgga agcgaaaata tgagtaagat gaaaatgtta aagccatcag atggattgga
     9601 aggaattaaa aaattcataa tagatacagt ttcagaagct ggacctaacc catgccctcc
     9661 tatggtaatt ggagttggta taggtgggac tgtggacaaa tgtgctcaaa tagctaaaaa
     9721 ggcacttttt agagaactag gtgaatttaa taaggacgaa aatatagcaa aacttgaaag
     9781 tgaactatta acagctatta ataaacttgg tataggacct caaggcttag gtggtactac
     9841 aacagctttg ggattaaaca tagagacttt tcctacacac atagcaggtt tacctgttgt
     9901 tgtaaatata aactgccatg catcaagaca taagaaggta gtaatttagg aggaaaataa
     9961 aatgataaaa ataactacac ctgtaaatga aattgatata gcaaaattaa attgtggaga
    10021 tacaattagt ttgagtggta tactttatac agcaagagat gctgctcata aaagattgat
    10081 agattgtata aataaaggtg aagaattacc atttgacgta tatggtcaag gaatatatta
    10141 tgtaggacct actcctacaa agccaggaga agttataggt gctgcaggtc caacgacgag
    10201 ttatagaatg gatgatttga ctatacctct tttagaaaga ggtcttagat tgatgatagg
    10261 caagggaaaa agaagtgatg aagttataga aggtatgcaa aaatacggag cagtttatct
    10321 tgctgcaata ggaggagctg gagcatatat ttcaaattca ataaaatcat gtgaaattat
    10381 tgcatatgaa gatttaggag ctgaagctat aagaaagatt gtagtagaag accttaagct
    10441 tacagtagca atagatagct atggaaataa tatatatgag caaggtcgag caatttatga
    10501 atgtaaataa tgtttagaaa taaatatgaa tatcaaaatt aaaaatagtt gtgtatattt
    10561 taaggaggaa aattgatgtc taaggattat gcaaaattgg cattagaaat gcatgaaatc
    10621 aacaaaggga aagtaagtgt agaaagcaag gtagaagtta aaactaagga tgatttatca
    10681 acagcatata caccaggggt tgctgaacct tgtttaaaaa tacatgaaaa tcaagatgat
    10741 gtttatagat acacttctaa agggaatcta gtagcagtag ttagtgatgg aagtgctgta
    10801 cttggattag gtaacattgg tgctgaagca tctatacctg ttatggaagg taaagctata
    10861 ttatttaaac aatttgcaga tgtagacgct tttccaatat gtttaaagac taatgatgta
    10921 gatgagatag ttaaatctgt agagttgatg gagcctgtat ttggaggtat aaatttggaa
    10981 gacatatctt ctccaagatg ttttgagata gaagagagat taaaaaaatc tttaagtata
    11041 cctgtttttc atgatgacca acatgggaca gcaataatag ttgcagcagc cataataaac
    11101 tcaataaagt taattgaaaa taaaaaaata gaagatttag aaatagttat aaacggagca
    11161 ggagctgctg gtatagccat agcaaagata ctacttaata tgaatgttaa aaatataatt
    11221 ttatgtgata gaagtggagc attggaagct agtatagaaa atctaaacta tgttcaaaaa
    11281 gaaatgttaa aagtaacaaa tataagaaat gaaaaaggac ctttaaaaga tattataaaa
    11341 ggaaaagatg tatttattgg tgtttcaggt ccaggagctg ttacaaagga aatggttgaa
    11401 agtatgtctg agaagccaat aatattggca atggcaaatc ctactccaga gataatgcca
    11461 gaagaagcaa aattaggtgg agcatttata atgggaacag gtcgttcaga cttccctaat
    11521 caagtcaata atgtactggc ttttcctgga atatttagag gtgcacttga tgttaaggct
    11581 aaagagataa atgaagaaat gaagatagct gctgcatatg caattgctaa tacaataagt
    11641 gatgaagaaa tatgcccaga gtatatatta ccagatgtat ttaataagaa tgtagtaaaa
    11701 aatgttgcta aggcagttaa agaagcagcg ataaaaacca aagtaaatag aatataaatt
    11761 tttatatgtt catatatcct tatcttatcg ggtatatggg catttttatt ataggtttat
    11821 actcatatat ccttactttt atatacatat ctttatgatt aaaggtgtta gaaaatgtag
    11881 taaattttat actatcagac tctatcttta ttactttagt agatacatgg tggtaaaggt
    11941 ttatagaaaa aaatatagga aggcagattt ttattgttgc gtatatatga gaatgatata
    12001 atatgaatat tataaattaa taaatttaaa tttatggagg tacaagagat gatggaatat
    12061 aaagttaatg accaagaact taatgcttca atgtttatcc catttgtcaa caggatatgg
    12121 ccaggagatt ataatatgga taaaacacag actgcattat caaaaacttt gaatataact
    12181 gcttatgatg ataaagtgct tgtaggatgt ctgcgagttc tttcagatgg ttattatttt
    12241 ggaacaatta cagaattgct tgttcttcca gagtatcaga aacaaggcat tggtagtaag
    12301 cttttccagc ttgctaaaga gaacacccca actatgctat attttggtgc acagccagga
    12361 gtagaagagt tctatgaaaa aaatgggtgc caaagaagtc tgcaatccta cataataaaa
    12421 aagaaagata gttattaaag ttccagcttt caataatctt aaaagtacaa tattttagtg
    12481 gaatcatttc attttttatt actaatatga cataaacatg tcatattagc attgatatac
    12541 ttttaatatg gttaagagcc aaagaaaaat ataaggagtg atttaaaatg gaagaaacta
    12601 aattttgcca atgctgtgca atgccaatgg gacaaactga tgaattatat gggacaaata
    12661 aagatggaag taaaagtact gattattgta gttattgttt taaagatggt gagtttacag
    12721 ctgatataag tatggaagag atgatagaag tttgtatccc tcatatgctt caaagtaata
    12781 aggatatgac tgaagatgaa gcaagaacaa tgatgaacaa tttctttcca acattaaaac
    12841 gctggaaata attttatgca aaattagtta tatgcaaaac atgaaatatg gtgttgagaa
    12901 taaggggtgg tgtgctttat agcataccac ctgttttgtt acttggagtt cagttatttt
    12961 cctaaatatt ttaaatatac tattttagat aagttccttc ttcatcttga taaattaatc
    13021 cttttttcat aagcactcca agagctcgtt taaaattttt cttacttgaa ttaaataata
    13081 tagaaatatc tttagggtca gatttatcat taaatctcat atatccatca gaaccttcta
    13141 aataacttaa aatcttattt tctaaagtat caagttcatc ctttctattt cctctaggag
    13201 aaagaccaag ttttccatct tcatatattt ttatgacatg taaattttca agaacatcac
    13261 cagtttttag ttcagtaaag tactcattat gaagtattac accagcatat ttattgtcta
    13321 cacatatcat tgcagaatta ttagtttgga atccatatac aactccagtt acaatatctc
    13381 ctacattgta agtatggtct gttaataagt atgaatctat atctgtagta gcagcaattc
    13441 ttttgctttt gtctaaatat atataaaata aatatttttc gtctctaaat agtggataag
    13501 tcttagcttt aaaaggaact aatatgtctt taggaagtcc catatctata aaagtaccaa
    13561 tgtctgtatt atcaactact tttaaatgtg ctacatcccc aaccttagct aaaggcggta
    13621 ttaaagttgc agcagttctg tcatcagaat ctttaaatat gaaagcattt acttcatcac
    13681 ctatgtttat ttcatttttt ccgattaatc tattatgaag aagtatatcg tcttttgtgt
    13741 tattagtttg tccatccaaa aaatatccaa attcagtttt tctttttact gtaagtttat
    13801 taaaatcacc tatgtttatc aaattaaaat catcctctca ttttatattt ataatttagc
    13861 cataatatac ttatgtagta gtttgtcaca gtatctatat tagtattagt gaaatttaca
    13921 ataaataata tagttattta tgtatttttc aattctctag cttcttatat agtataaact
    13981 ccaatttctt ttttgtcttt aggttttatt aaaattttta caaaataagt taaaaagtat
    14041 tgaaataaaa tttcttttaa agtataattg gtatatacca aaattggtat ataccatatc
    14101 gaaaagtggt gactatatga aaatagtaaa taaatctagt aatataccat tgcatactca
    14161 attaagttca ataataagag agatgataga aactggtgag cttaaagagg gcgatgcaat
    14221 aatgccagaa agagaacttt gcaacataca aaatgtaagt cgtatgacag taaataaaac
    14281 cattgttgga ttagttacag aaggacttct ttatagggta caaggtaaag ggacattcgt
    14341 tgcaaagcaa aaaaagaagt atcagttttc aaatgtaaaa ggttttactg atgttatgaa
    14401 agaaaaggga gtaaacataa aaacggatat attatccttt gagatggaac taccagatga
    14461 tttagttaaa agaaagcttg gaataagtga taatactaca aatatatata agattgtcag
    14521 acttagatat actgatggag agccatttgg attagaaata gtatatctat cagaagaaat
    14581 gtgtaagggt ctaacaaaag ggatattgga taattcttca ttatataggg ttttaaatga
    14641 aaaatatggc tataagatac aaaaagctga acaggtaatg gagccagtaa tactttcaga
    14701 tgaagaaagt aagcttcttg aaacagatga aggtgcttta gctcttaaac ttcatagaaa
    14761 ttcctataat agagaaggta gccctataga atataccata tcaatattta gaacagataa
    14821 atatcaatat gaaatagtat tgagtgagta ggtgaataat atgaaatgtt taattaatgg
    14881 aaagataata ttaaaaaatc aaatcttaga aaataaagta ttagtatttg atgaaaaaat
    14941 aattgatata gctgatagtg taccaaaaga ttgtgaagtt atagatgctg atggaaaata
    15001 tatatctcca gggcttattg atattcatat acatggaaac atgggaaaag atacaatgga
    15061 ttcgactgat gaatctattg aaacaatttc aaaatcaatt atgagacatg gagtaacatc
    15121 ttttctgcca actacaatga ctatggataa agaacatgtt tatgatgcac ttgaagttat
    15181 taaaaaagcc caaaatagaa agcttgaagg ggcaaaggta ttaggagcac atttagaagg
    15241 accttttata aatgagaatt ataagggagc acaaaatgaa aaatttataa taaattcaaa
    15301 atacgaattt ataaaagaat ataaagatgt tataaaagtt ataacttatg ctccagaaaa
    15361 agatattgat tttgatttta ctagagaaat aaaaagatgt acagatatag ttttatctat
    15421 tggtcatagt aatgcaaatt atgaccaagc taaagaagct ataaatttag gagtaactaa
    15481 tgtaactcac atgttcaatg caatgactgg tttaaaccat agagacccag gcgttgttgg
    15541 tgcagcactt acaactaatg tgtacagtga gttaatagca gatacaatcc atataaataa
    15601 agatttattt caatttatac taaataataa agggaaagaa agactgattt taataacaga
    15661 ttctatagaa gctggtggtc ttgaagatgg caattattct cttggaggtc agaaagtcat
    15721 agttaagggt aatgaagcta gacttgaaaa tggagcatta gctggaagtg tgttgtcttt
    15781 aaataaaatg gtatttaact tcttagataa tacaaacctt aaagtgaatg aagcaattaa
    15841 tttagcttct ttaaatccag caacatcttt gggaataaat gacaaaaaag gaagcttaga
    15901 aataggtaag gatgcagata tagctgtatt tgatgagaat ttagattgta aaatgacact
    15961 ttgcttgggg gaagtagtat ataaaaatat atagtataca aaacaaacct taaagacagt
    16021 aaaaacaata taaaaaataa taaattaaac ttacattagt aaaagtagta gttaaaattt
    16081 aggaggaata agatgagagt attagtgtgt aaagattatg atggaatgag taaaaaagcc
    16141 gctgaaatga tagcagctca aatagtttta aaaccaaatt caatactggg actagctaca
    16201 ggaagtacac cagtaggtat gtatagagat ttagtaaaaa aatacaatga taatatagta
    16261 gatttttcag atgtgatgag ctttaattta gatgaatatt ataagttacc aatatctaat
    16321 gaccaaagct atgattattt tatgaaagag aatctattta atcatgtaaa cataaagcca
    16381 gaaaatactc atctaccaaa tggaatggct gatgatattg aaaaagagtg tatgaactat
    16441 gaagcttcta tagatgcagc tggtggtata gatgtacaag tacttggaat aggtcgtaat
    16501 gctcatatag gttttaatga acctgatact aaatttgcaa aaagaactca tgtagttgaa
    16561 cttacagagt ctacaataga agctaatgca agattcttta aatcaagaga agatgttcct
    16621 aaaaaagctg taagtatggg aataggttca atattgaagt ctaagaaaat attgctttta
    16681 gcttcaggag aagaaaaagc tgatgcagta tataatactg tttacggaga tataactcca
    16741 gaagttcctg gttctatact tcaacttcat aaagacacta tagttatagt agatgaagca
    16801 gcagcaagta aactgaatcc aaaagattat aaattagtat aagaatagaa tgtagtttaa
    16861 gtaaaaaata gctatcattg agtaatcaaa tttatttaat gatagctatt tttatttaaa
    16921 ttttttactt ctttttattt aaattagtta ttattgttta aattacttgc ttattacttt
    16981 ttacgaaaac tcgttatcaa gaagtatcca aataagatgt taaaaattat tatgtttagt
    17041 gggattctat caagcataat aaaattacct ataattggga tattaccaac tggaaacata
    17101 aagaagaata caggcatcag tgatagtaaa aaagatggaa aacaaagaag aagtaatctt
    17161 ggaatatcaa tataccaact accatccttt ttgtattctc taatcaataa ttcagttcct
    17221 agtaataaac caataccacc agaggaaatt aaattaataa aaatgtatct tttaaagtct
    17281 tgcatataac cagtgtgaaa ttgagtatag aaatcataat ttaatttaac ggctaaaaaa
    17341 tacataagca atagaatgat taaaaataaa agatatctaa gagctttttt cattttaaat
    17401 tctcccttca gaaatacttt atttagtttt attttgttgg aatctgattt tatcatttag
    17461 tttaagaaat ccataatatt agtgaagaat gaaataagta t
//
LOCUS       DAEMIK010000086        17485 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEMIK010000086 DAEMIK010000000
VERSION     DAEMIK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.086"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..78)
                     /locus_tag="KPK87_002375"
     CDS             complement(<1..78)
                     /locus_tag="KPK87_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2538520.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILN"
     gene            complement(91..1287)
                     /locus_tag="KPK87_002376"
     CDS             complement(91..1287)
                     /locus_tag="KPK87_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2538521.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELVRFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTCILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(1534..1938)
                     /locus_tag="KPK87_002377"
     CDS             complement(1534..1938)
                     /locus_tag="KPK87_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538522.1"
                     /translation="MGYLQMQLMNETTETITIGICLNIDKFYADLDFTANSDFEKWDE
                     YPIERFHEKMRVILEEMENKQQELPLLFSIPLDEEMSFLNITFCYNFIVMDKALFLHT
                     QHEFELDSEALEVCRNEDIGCVVLYLGMNTVM"
     gene            complement(1949..3328)
                     /locus_tag="KPK87_002378"
     CDS             complement(1949..3328)
                     /locus_tag="KPK87_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538523.1"
                     /translation="MRKIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNINQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLAQM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGRLAQDRQ
                     VMPEQQEKE"
     gene            complement(3417..3812)
                     /locus_tag="KPK87_002379"
     CDS             complement(3417..3812)
                     /locus_tag="KPK87_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538524.1"
                     /translation="MLKEQDIKQYLNEKGSTFKENTSIIGVIFPSKFTYTLGPVATAL
                     SMQYYALNFSDSGIAIIGLNNVTGKLENDAFLFVLKEEITSVKFNKKLMSYELEISTS
                     KGTLAFKVNKTMVGASWHKENLATILKSL"
     gene            complement(4081..4605)
                     /locus_tag="KPK87_002380"
     CDS             complement(4081..4605)
                     /locus_tag="KPK87_002380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538525.1"
                     /translation="MIYEPEKLKNKRTMYKKKDKMIVSIEFLLWAIILFAYVNIVIPY
                     VKSTIAFLTIIIGGIAIITAVYFFIAVYVLINRGHRFRKINNAIVREYHENKNGELFL
                     EKLFEIEEKPTDMNDEITWYLNIATAFSVLGKKNESISLLKQLEEVVTGKDKEYIQNS
                     IKFVKEQSEKDDTH"
     gene            complement(4602..5192)
                     /locus_tag="KPK87_002381"
     CDS             complement(4602..5192)
                     /locus_tag="KPK87_002381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538526.1"
                     /translation="MAIIIYILTILLAVATFFIIKNKRYKVVPLVSWVIIIFFIGCVF
                     ISIVSLLVPYGEIFVLPLGSIVLGFVVILAAFNDFYSLIRCKDKIEGIYRGYNTYYGG
                     NGVSTQAPVFEYMYNGTKYNEQTTQNISYKLLTQNMRNGETYSIYVDPKHPAVFILQK
                     KIKVSSIITLIFGIMFFTAGIATLCAIFPVFWSVIK"
     gene            complement(5228..6040)
                     /locus_tag="KPK87_002382"
     CDS             complement(5228..6040)
                     /locus_tag="KPK87_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5037 domain-containing protein"
                     /protein_id="HBG2538527.1"
                     /translation="MRYKKFLLGVIASVLCLELLNGCGHKNEISIKIETYLQEEYGEE
                     FEVLSWKQQKLLPSDNGAIHATCISKNDPKHPFEGSYFNPEEPNSKIEIIDDGYAERL
                     LAKQMESMTEEAISQVAENYYIQGAIIIPEEWQDIPVEEISQWKNYVDLCNQSNSDYK
                     TLGSVWVYIDASTMTGKTDEEEYQMYEEVFRDKLGGQALLYVYYLDHKRFEKAEEILE
                     ISAPHKEGSNFEDIIEGQPYFGTIMRYGSDKFDDSLEIFKAAKQGQEQEIFQ"
     gene            complement(6155..6397)
                     /locus_tag="KPK87_002383"
     CDS             complement(6155..6397)
                     /locus_tag="KPK87_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538528.1"
                     /translation="MNIKEAIKMIQDEKLQGYNMNERKDDRENEVVLMRENDKWIVYA
                     TDERASKVTNSQDIYLDEEEALDDFIDRLRAFNRLR"
     gene            complement(6505..6888)
                     /locus_tag="KPK87_002384"
     CDS             complement(6505..6888)
                     /locus_tag="KPK87_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2538529.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHTPQGQPKK"
     gene            complement(6904..7230)
                     /locus_tag="KPK87_002385"
     CDS             complement(6904..7230)
                     /locus_tag="KPK87_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2538530.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            complement(7469..10513)
                     /locus_tag="KPK87_002386"
     CDS             complement(7469..10513)
                     /locus_tag="KPK87_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2538531.1"
                     /translation="MKKTWKRLCTGFLALATVVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGYMKVEGETAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFADKDCTKQLATLTTDENGNTDIAEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKGNLPAEATRTWVTKTIAETHSDGTTHYITKLADTYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLEGAYMQAGDKSEQIKGLYLTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSSDLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVVDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLNGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADEEEMKVEISYTFNASALGGKNLVTFEELYD
                     FSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKNGNKELEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPPTPEKPN
                     RPSDSPKTGDSTNVMAFIVMLLASAGGLAGTYLYKRRKMKKS"
     gene            10675..11481
                     /locus_tag="KPK87_002387"
                     /pseudo
     CDS             10675..11481
                     /locus_tag="KPK87_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            11465..12235
                     /locus_tag="KPK87_002388"
     CDS             11465..12235
                     /locus_tag="KPK87_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538532.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            12245..13027
                     /locus_tag="KPK87_002389"
     CDS             12245..13027
                     /locus_tag="KPK87_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538533.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVNSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            13404..13940
                     /locus_tag="KPK87_002390"
     CDS             13404..13940
                     /locus_tag="KPK87_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG2538534.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            13972..14361
                     /locus_tag="KPK87_002391"
     CDS             13972..14361
                     /locus_tag="KPK87_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG2538535.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            14398..14784
                     /locus_tag="KPK87_002392"
     CDS             14398..14784
                     /locus_tag="KPK87_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG2538536.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            14911..16353
                     /locus_tag="KPK87_002393"
     CDS             14911..16353
                     /locus_tag="KPK87_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2538537.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            16650..17465
                     /locus_tag="KPK87_002394"
     CDS             16650..17465
                     /locus_tag="KPK87_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2538538.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5314 a         3189 c         3074 g         5908 t
ORIGIN      
        1 attcaaaata cacatcaaga ctacgccgat acccattccc atagatacca acaggaagtc
       61 ttttaataat acccacattt tttatcactc ctaactttcc accaaatctt ttgcaggggt
      121 cgtctgctgt ttgattatca tttcatgctt ttctgtgagc tttgcctgct gttcgattgt
      181 ttccatgtag tctgtaccgt ttcccttatc aatctttttc agcattttca aggttggtgc
      241 tacctgcctt tgtacccacc gcaatgtacg gtctaaggtg taaggttctg gctttgtcgt
      301 cagcttcaag ctctgtctgt tatcgccaat aaaccaagcc cagcggtcat tgagtttcca
      361 gtcatttttt cgcttgtctg gttcttcatc aacaaaccgc acatactggt tgatgataga
      421 aaaggcggtc tgttctgcgt cataataagt cagcaaatct cgtactgcat aataggcacg
      481 ttcatttcta agccgtatct caaaacgatt gataatgtcg gcttcttcaa gcggtgtccc
      541 taacttgacg tactgctcat agtccttttc atagatacag aaatacacat ctgatttcag
      601 cgaaccaaga taaagggtac gccccatata ttctctgtcg tcctctctgt gcttgataag
      661 ctcgcccgac tggtaaaact tatagcttct ggactttccg atatattccc gtttcctgca
      721 tttttccgca agctctggaa tatccaaaat gcacgtatgg tcgttgatag caaggtcgat
      781 acgcttcatc acgccaccgt ctatgagtgc gtccataaga aagtcatacc agcttctttg
      841 ctgtgccagc aggtaacttt caaactgcct acaaccacgc ccttttaatt ctaaaaggac
      901 acctttttct tcgtcagccg acgtatagat aaagatgtcc cctaaagaat aatgctccgt
      961 ataactgtaa tgtccgtaat cttcatggag catataattg atattcaatt ttaatatatc
     1021 tttgatgatg tgctgtatat ccagcgtggg aaaacgaatt ttcacataat caaacagcat
     1081 ggtaagtggt gcttctggat tgaaccttac cagttcttta tgcagagttt ccagaagttc
     1141 ttttgacggc ttcatttttc cgctttctat cttgttgaga tattctcttg tgataccaga
     1201 agccaccgcc agtctgcctt gtgagatacc gtaagcaatc cgtttctccc gtaattcttt
     1261 tatccattgt tcttcattca gaaccatacc cccaatctgt caagtgtgaa gttttttaag
     1321 gcgacttcac agccgatttt tgctagaaaa tcatgccaga agccttatgt ttcctatgtt
     1381 ttttgtctat tgtctatttg caaacgtacc cctctgttag ataccgaggg gttttacctg
     1441 ctggcgtggc tgacgccaca ccagcaacgg ctagtccgtg ccgtcgcctt tcgcttcgca
     1501 cgtcgccgtc ccgtcctgcc ttgcggtatg agcctacata acagtattca taccgagata
     1561 cagcacaaca cagcctatat cttcattcct gcatacttct aaggcttcgc tatcaagttc
     1621 aaattcatgt tgtgtatgca gaaacaaggc tttatccatg acaatgaaat tgtagcaaaa
     1681 tgtaatattt aggaatgaca tttcttcgtc cagaggaata gaaaataaca gcggtaattc
     1741 ctgctgtttg ttttccattt cttctaaaat tactctcatt ttttcatgga aacgctcaat
     1801 agggtattcg tcccattttt caaagtcgct atttgccgta aaatctaaat cagcataaaa
     1861 tttatcaatg tttaagcaaa taccgattgt aattgtttct gttgtttcat tcattagttg
     1921 catttgtaaa taacccatag ttattctttc actccttttc ttgttgttct ggcataactt
     1981 gtctgtcttg tgcaagtctg ccgatagtct gtaaaaaatc atgtcctttc gggactaaag
     2041 gcgtgtaaaa ttcactgata acgcttgttc ccacatcaca atagccacgt cccttgatac
     2101 gcttctgaaa aaactgtttt ttcacatctg aaccgaacag cataccgtaa cctagttcgc
     2161 tgatacgccc caatcccaca cgaaaattga agttatctct gataccgtcc gagaaatact
     2221 ttgcgtctgg acgctggcag gcaacgataa ggaaatatcc tgcttgtcgt cctaacataa
     2281 cgattttctt taactggcta agtaagctca cgctttcttt cgtccccagc atttcaaaaa
     2341 atgccacata ttcatcaaag ataagaaagc agggtggcag tcccagatag gcgtagtttt
     2401 cgccagtctt atagttcggg tgtcgcttca tttcctcact tcgctgtacc atgccctcat
     2461 aaaaggcatt gacgcaatct atcatttctt ctttggtgtg atatacattt cccataactg
     2521 ttcctaagtc cgcaaggtca gcgttcttcg ggtctaagat ataaaggaca gcgtttgtat
     2581 gcagtaaggc ttcaataagc gtcagcaaaa aataagtttt accgccacca gtcccaccag
     2641 caatcagagc gtgaggaagt gcgtcatatt cccagacaag atttttcatc agtctaagac
     2701 agccgttttc tgcccgtact tcatcaatgg taatgcggtt cgctatcata tcataaagca
     2761 gggtatattc gatatagccg tcatgcaggg tcttgtcggt cagctcacaa tacaagccac
     2821 tttccaattt atcctctaac cgtaaaagct ggtcttgata ttttcctaat gtaatttcac
     2881 agcggatatg aagcagtccc ttttccattt gataataaat ctttggaaac cagacgattt
     2941 tttcccttga tctgctttgc aggtcagtaa aaaaaccgct gtcttgtacg gtgtccgctt
     3001 cataccactt gttttccagt atcatttgtg ccagcttttg acggtgcagg agcttcttga
     3061 aactgtcgta acagaagcgg taatacagaa aagcgactaa tgcacaaaca ccagtcgcta
     3121 tcagtatggt tatgaaattg taaggggaga gcgtcaagcc gttttctaac aagctgaaat
     3181 gctcccagtc ggtacgcatg agctggttga tattcaatag caatagaacc gccacgaata
     3241 caaacagaag cgtccctatg gaaaaatgat agacaaggtg tttgtcgctg gctctgatac
     3301 ggtgtccttt gttccaaatc ttacgcataa atcaatcact cctttctggg tatgaaaaaa
     3361 gcagtcaatc ctaagactaa ctgctttata tgtatagata tgaatttttg aaaaacttat
     3421 aaactcttta aaattgtagc taaattttct ttatgccatg aagcacccac cattgttttg
     3481 ttcactttaa aagccaatgt tccttttgat gtactaattt ctaattcata agacattaac
     3541 tttttgttga atttaacact tgttatttct tcctttagca cgaaaagaaa cgcatcattt
     3601 tcgagttttc cagttacatt atttaaaccg ataatagcaa taccactatc gctaaaattc
     3661 aatgcgtaat attgcattga aagagcagtt gcaactggac cgagagtata tgtaaactta
     3721 ctagggaaga taacaccaat aatagatgta ttctccttaa aggtactacc tttctcgttg
     3781 aggtattgtt taatgtcttg ttcttttagc attttaattt tcctcctata aaattatttt
     3841 tcgcataact tactgtttac attttatcat ttcaatatag tgattacaat gtaaatatta
     3901 tatctaattt gcgattatcc gcataaacac tatactttta agcattttga aatataagaa
     3961 aatactcatt ttaccacgaa tttaccacga ttgaatgagc cttgatatga cactaaaaat
     4021 aacatgggag atggtacaaa atctggctat ctcccgtctt tcttaatttt attgtaacgc
     4081 ttaatgagta tcgtcttttt ctgattgttc tttcacaaat ttgatactat tttgaatata
     4141 ttctttatct tttccagtta caacttcttc taattgtttc aataaagaaa tgctttcatt
     4201 tttcttacct aatacgctaa atgcggttgc aatattaaga taccatgtta tttcatcatt
     4261 catatcggta ggtttttctt ctatttcaaa taatttttct aagaaaagtt ctccgttttt
     4321 attctcatga tattctctta caatagcatt atttattttt ctaaagcgat gtcctctgtt
     4381 tattaaaaca taaacagcaa taaaaaaata aacagctgta attatagcaa ttcctccaat
     4441 tataatagtt aaaaaggcga ttgttgattt cacataagga attactatgt tcacataagc
     4501 aaataaaatt atagcccata ataaaaattc gatagaaaca atcattttat cctttttctt
     4561 atacatagtt ctcttatttt ttaatttttc tggttcataa atcatttaat cacactccaa
     4621 aacacaggga aaattgcaca tagtgtagca atgccagctg taaaaaacat aattccaaat
     4681 ataagtgtaa ttatagagct cactttaatc tttttttgga gaataaatac tgctggatgc
     4741 ttcggatcaa catagatact gtaagtttct ccattcctca tattttgcgt taaaagctta
     4801 tatgaaatat tttgtgttgt ctgttcgtta tactttgttc cattatacat atattcaaaa
     4861 acaggtgctt gggtagaaac tccattccct ccgtaatagg tgttatagcc acgataaata
     4921 ccttctatct tatctttaca tcttattaaa gaataaaaat cgttaaacgc agctaagata
     4981 actacaaacc ccaagacaat acttcctaaa ggtaatacaa atatttctcc atatggaacc
     5041 aataaagata ctatcgaaat aaatacgcat ccaataaaaa atataataat aacccaacta
     5101 acaagcggta caactttata gcgtttattc ttaataataa agaatgttgc aactgctaac
     5161 aatatcgtta gtatataaat aatgattgcc atatccgttt tcccaatacc ctttctttca
     5221 atcttactta ctgaaatatt tcttgttctt gaccttgttt tgcagccttg aatatctcta
     5281 agctatcatc aaacttatct gaaccatatc gcatgattgt tccgaagtat ggctgacctt
     5341 cgataatatc ttcaaaatta ctgccctctt tatgcggtgc tgaaatttcc aatatttctt
     5401 ctgctttttc aaatctctta tggtctaaat agtaaacata aagcaatgcc tgaccaccta
     5461 atttatcacg aaagacttct tcatacattt gatattcttc ttcatcagtt tttccagtca
     5521 ttgtagaagc atcaatatag acccaaaccg aacctaaagt tttataatcc gaattacttt
     5581 gattacatag atcaacataa tttttccatt gtgaaatttc ctccactggg atgtcttgcc
     5641 attcttcagg tataataata gcaccttgaa tataatagtt ctcagctacc tgcgaaatgg
     5701 cttcttctgt catagactcc atttgttttg ccaaaagacg ttccgcatag ccatcatcaa
     5761 taatttctat ttttgagttt ggttcttcgg gattaaaata gctaccctca aatggatgct
     5821 ttgggtcatt ttttgaaata caagtcgcat ggattgcacc attatctgat ggtaaaagtt
     5881 tttgttgttt ccaagacaac acttcaaatt cttctccata ttcttcttgt aagtaggttt
     5941 caatttttat tgaaatctcg tttttatgtc cacagccatt caatagctct aaacaaagca
     6001 cacttgctat aacgcctaat aaaaattttt tatatcgcat aatcgccacc tctatgtgat
     6061 tttatgattt aacacagttc tacaaactgg aagttgtcta tttaaaagaa aattactaaa
     6121 tatctttttt gaggtcattt taattctccc atagctacct taatctatta aaagctctta
     6181 atctatcaat aaaatcatct aaggcttctt cctcatctaa ataaatatct tgtgaatttg
     6241 taaccttact agcccgttca tctgttgcat aaacaatcca cttatcattc tcacgcatta
     6301 acacaacttc attttctcta tcatctttcc tttcattcat attataacct tgtaatttct
     6361 cgtcctgtat catttttatt gcttctttta tgttcatgtt tcatgctcct atttttgtac
     6421 tacaacttct aatttgtccg tttcttcata tccatattat aacagttcca tatatccgct
     6481 acaattaaaa tctattcagc aagcctattt cttcggctgt ccttgcggtg tatggttctg
     6541 tggattgcct gcgttctggt tgcctttatt cttcaataca atatcctctg cctttacata
     6601 ccagtccaca tctgcacccg tataggtctt tcttgatacc gtatcggcta ctggattgac
     6661 aagctccacg actgcattgt acggaaattc ccttaacggt acttctggcg gtacggacac
     6721 ggggataatg ccaccatgca gactgcactt caaatcatag atacgttttt ttagctgggt
     6781 actcggtgtc ccgtcctcat tctgcaagaa tacatcacgc accgctgtaa attttaattc
     6841 tccaaatgtt ttttctttgt caataacaaa cccgtttgat aatctcataa attcttcact
     6901 cctttacttt tcttcaactg ctacaatatc atcagcaact aacacataat cggtatgtcc
     6961 catatccccg attgcgtaac ctctgccgta taacttcgga ttgacaagtt ttactttctg
     7021 ctcatacttg aaatgctttt cgccagcctg cacgggaatt tctaccacaa cattttcgcc
     7081 tttctgtacg tcagaataaa ggttatagct tcgtctggct aaaaccctgc ggttattttt
     7141 atctctttca aagactggct cactttcgcc tgcaaattca agagttccaa aagactgtgc
     7201 catatctggc acgacatatt tcatttccat attgttttac ctcgtttctt tctatatttg
     7261 ataagtttat tgattgtgat ttcttattct ggcggtttgg gaagtaccag caatccccca
     7321 gatagtaaag ggtaaaatca atgcttacgc acccttgact atccgtgttc ttgcaggttg
     7381 ctggcagaca agccagaata agcaaaagtc tgccgtttga cagcttcggc ggagagcgtg
     7441 atttttcttt catcataccg ttaccgcctt atgatttctt cattttacgg cgtttgtaaa
     7501 gatatgttcc agccaatcca ccagcagacg caagcaacat cacgataaat gccattacat
     7561 ttgtactgtc gcccgttttg ggactgtcgc taggtctgtt aggcttttct ggtgtcggtg
     7621 gtgtttcggg tttctctggt tcttctggct tttccttaaa ggtaattgtc tgtcccttat
     7681 cctcaatatc cttatgctcg gtaactttct tcggttcgtc tggattgctc aaatcgtaca
     7741 attcttcaaa agttacaagc tgtttaccgt caagagaagt agcatcaaag gtaaaggcaa
     7801 cttccacttt catagtttcg ctgtcagcgg taaacgtata atcactttcc acacgctttc
     7861 cattgataag aagttctgca ttttcttctt tcaacatctg ccagcccaca agtttgtact
     7921 gtgtaccgac ttctaagccc tctaaggtta cggtatcaat gattgtaacg tcttttccag
     7981 cttcaagctc tttgttgccg ttcttatccg tagcagtcgt atgaattttg atgatacgct
     8041 ctgtgataag taccgtctgt ccgtcgtcct caatgtcttt gtgttctgca actttcacgg
     8101 gttcgtctgg attgctgaaa tcatacaatt cttcaaaggt aacaaggttt ttgccaccta
     8161 aagcagacgc attgaatgta taggaaattt ccactttcat ttcttcctca tcagcgacaa
     8221 aggtataatc gttttctaca cgtttcccgt caatgataag ctcggcgttt tcttccttta
     8281 acatctgcca gcctttgagc tgatactttg tgccttttgt aagtccgttt aatttgacag
     8341 tatcaacaat cgtaacctct tttcctgcaa ggatagtctt ttcgccgtcc ttgctggtcg
     8401 ctgtggtatg gatagagatt tctttttcgt attcatcagt caaagtccct aaatcaacca
     8461 caaggttatt tcttgatacc acgatttcaa aaggtgggat aagttcaaag cctttgttac
     8521 tatcagagcg taattcttca atgatgtaag tatcgtaagg taacgcacct ttgctgtcgt
     8581 ctggttcaga agttccaaac cacacaccgt cctcgctggt cttgcctgcg tttgtattgt
     8641 gcttatgaga agcccagtca gcagaagtgg aaaactgccc gttatcatca gttaccacta
     8701 catgattttc gcccgttgtc ttgcttgtga tccgaaaggg aacatcagca agacgcttgt
     8761 gtgtacctgc accgattttc acgccctcaa tatctccacg cttaatctga ttgtagatag
     8821 aatgggcttt gtcggttaag tccacgattt ttccattttc tgtgattgta aaatcaatcg
     8881 gttttgcacc gtcagttaag taaccgtcgg gagcttcact ttcaacgata cgaaattttc
     8941 cataaggtaa aaggtcagaa gaagtagaag cgacaccctc aatatcggta cgaattgtct
     9001 ttaccacttc atttttctta tatagcttgc cctcaaccaa aacagaatta tcatttaagg
     9061 aaatgatgtc aaaggcagta tctttcaagg ttgcacttcc ttgtggcttt gtatcgcccg
     9121 tttctaaatc tcgtttctga atttttacac cgccacggat aaccttgtct gatacggaaa
     9181 actggttact tccagatagt acggcaaggt cgccgtcctc ggtaatctgt gtaaggtata
     9241 agccttttat ctgttcggac ttatcgccag cctgcatata tgcaccctct aacaagtagc
     9301 cgtttggagc ttttgtttct tcaacggtta gtgtcccaag tggaagaacg gcttttccgt
     9361 cttgcatata gaagctgtcg ccagatactt tgtatgtgtc cgctaatttt gtgatgtaat
     9421 gagttgtccc atcgctgtgt gtttcagcga ttgtctttgt aacccatgta cgagtagctt
     9481 cggcagggag attgcctttg ttatagaagc ctgcataata cttccatgta aattccgcac
     9541 ctgctaaaga agcgtttccc tgcggattgt ctttctgtgt ttccatatca atcttgaaaa
     9601 gctcaatcaa agtgtccgtt actttcggtg tatctgatac tttcaaagtc gctgtctttc
     9661 cagcttcaac ctttaaggaa tatacagttt tatctacttt atatcctgct ggtgcggata
     9721 attccttgat atagactgtg cctgctttta cctctgcaat atctgtattt ccgttttcat
     9781 cagtcgtaag ggtggcaagc tgtttcgtgc agtccttatc agcaaagaca ccgtatgtcg
     9841 caccagcgat agagtaattc ccgttaccgt ctgtaatgct ggtattactg gaagtctttt
     9901 gtaatttcgc atttccgaca ttcagtttcg cccagaattg tcccaattcc tgcccctcgc
     9961 cagagtagat ataaccgcca cattcatagc gtcctttatt ttctttgaca aaggcttttg
    10021 caccagagaa aacttcgtcc tgcgttgcct ttggaatttc atcataagaa gctcgcacgt
    10081 tatcacattg ccagccaaga tgtacgctca atctctgcca aactacacat tgttccaaca
    10141 gatagacttg tttatagttc aattccttgt gagcttcgcc atactgtttg acatactcta
    10201 aggataacgc cacatctgaa atctggtcgg cactcatgcg tgagcttgcg tcagctctgg
    10261 tcttgtagcc gttcttaaaa tctgtattga tgtcaataca ataggcagtt tcgccctcga
    10321 ctttcatata gccctcattg aatgtcgaac cgatagaacc gtcattcatt actttttcaa
    10381 tgataccgac acgctctgca ctttccgtcc agtattgctt gctttctgca tgaacgggtg
    10441 tagtcggtaa agcagtaacg acagttgcaa gagctaagaa gcccgtacac aatcgtttcc
    10501 atgtcttttt cataaatcta atgctccttt ctgtaatcat cacaatttaa aaacgccact
    10561 tgtgaagtac aaatgacgtt ttaggtgtga taatgacttt tctttatctt tcttattttt
    10621 aatttttcct ttatttatgc ggtttttccg ctttgtctat ataagcatgt accgaacggt
    10681 gcgggtaaga cgacaactat gaaaatgtta ttgggtctaa caaaaccaac tactggagaa
    10741 attaaaatat ttggtcagga cattcacaat aacgagaaga agcttcttcc tcgaattgga
    10801 tgtttgattg aatcaccagg gttttatcct aatctgacag gaacagaaaa tctcaaaatc
    10861 tttgcaaggc ttagaggaat accaaaaaaa aatgcagtag aatcagcact ggatgtagtc
    10921 ggtttgccaa acaatgacaa aaagttattt tctcaatact ctcttggtat gaaacagagg
    10981 ttggcgattg ctcttgccat tatgcatgac ccacaactgt tgattttgga cgagcctatc
    11041 aatggacttg atcctattgg tattgcagaa gtaagaacgt ttattcgtga tctctgcaca
    11101 gaacgaggta aaactatttt gatttccagt cacattcttt ctgaaattgc cttgttggca
    11161 gatgacattg gcattattga ccatggtatt ttgttggagg aagaaagtct tgcccaatta
    11221 gaagaaaaaa acagtaaata catacatttt gctgtatctg atgcagcaca ggcagcaagg
    11281 cttctggaaa cccaacttaa cctgaaaaat ttcaaagtag cagataatta caatattatg
    11341 ctatatgata cagatgtttc tgttgcagaa ataaatcgtg ctttcattct aaacaacatt
    11401 gaggtatcag aatctcattt atgcgaagat actcttgaag attacttcaa gaaagtaaca
    11461 gggggtgaag gaattgcttg atataatttc atgtgaattt tcaaaactca agcggttaaa
    11521 atttatactg atttctattt taggtgcttg cttatttcca attcccgcaa caattttgat
    11581 tgcaaaggac aacttgccct ttgagcaatt gttcaaatta gttgtcaatt ttggatattt
    11641 tttgctatta ccaattgtat taagtattgt tgctagccaa ctatttttta tagaaagaga
    11701 taatgatgta ttaaaaaatc ttgcaacagt gccagtgcaa aaaggaaaac tggctctggc
    11761 aaaattggct gtgttgctgt ttatatcatt gttctactct gttgcagggt taggagctac
    11821 tatcattgga ggtttcattg ttggaatagt agagggggtt gctgtaaaat taggaatgag
    11881 cattgctttt ggaattatgt tttttgttac tgtacttcct gttgtggtac tcattgtata
    11941 tttcaatcgt agctatattt tttctattat actttctttt gtatatgcta ttttcaattt
    12001 tagtatatcc ttaaatatta tcaattttga accaaataac ccaatattaa gtgtattacc
    12061 tgctcctgtt ataatgcgtt ggtggatggc attttgggga gatcccactg gcgaatacac
    12121 ggcacttaga cagccatatt tgctctcaac tcctgcttgt gtaggtattt tatgtttgat
    12181 aaccattata gcagtcctgc tgatttgtat gatatacaaa aagcaggaga attaggaggt
    12241 atctatgctt gatataatta ctacagaatt tcaaaaaata aaaagatata atattctatg
    12301 gattggtgtt gttgctgttc tgttttcagc actattggca gtttttcagg ttgtaaattc
    12361 acatggcagt gaccctctaa cgtatgagag ctttgcaaac ggagttatct ggaataattt
    12421 cagtcttagt tttccatttg ggattacact tattggagga tatcttatta accgtgaata
    12481 tacagaccaa accttaaaaa acatcctaac tgttcctatt tcattacgca aattgctagt
    12541 gggaaaatta attgcggttg gaggaattac tgtattgtta agtcttttca gcttcttatg
    12601 cacatttatt ttggggttgt tattttgcaa tatcaatata tctgttacac tcgttgtaaa
    12661 atccttagtt cagattttgt ctgttaatct gtactgttat gttgctgttt tgccgattat
    12721 tgcttatttt ggtcgtaaac aaaatgcatt tctgacgggt gtagggattg catttgtata
    12781 cggtttttgt ggtgtgtttg ttgctggaag aaatctgaca gacttttatc caatcacagc
    12841 aggtcttggt ctagttggat acaacaatgg tgctggtact gtttatcagc cacttatcgg
    12901 tgctatgact ttgattgtaa ttctaatctt gacaactatt ctacttgtat ttacacctaa
    12961 ttatgataaa gttatgacca tatctaagaa aaaagggata aagcataaaa agtattctca
    13021 taaataacat aaatattgtt tctattattt aagttgctag acagtgaaaa gaaaaaatct
    13081 gtcgtacagt ggctatatgc tcatgtattc aagaagctac agttttgatt tttcataact
    13141 gtagcttctt tttgtattta ttaaatcacg actatcataa tacttgcaat aggtatagta
    13201 acaagagaga gtactgtcat atgtgtttta tatattacat taagtatatt ctaaaatata
    13261 tgttttaaat aatatatttg aaaataggaa tatttaaata aaaaaaataa tattatatat
    13321 agttgacaaa aattggaaaa tgagatacta tataaatata gaaatagtaa tcattactat
    13381 tattaaaaag ggaggaatta attatgaact taaaaggaac taaaacagaa aagaatttaa
    13441 tggcaggatt tgcaggagag tcagaagcaa gaaataaata tacatactac gcttcaaaag
    13501 caagaaaaga aggttataat caaatagctg ctatatttga agaaactgct aacaatgaaa
    13561 aggaacatgc taaattatgg tttaaacttt tacatgatgg tatgccttca acagaggaaa
    13621 atttaaagga tgcagcagca ggtgaaaact atgaatggac tgatatgtat gcaaaatttg
    13681 ctaaagaagc tagagaagaa ggatttgaca agatagctta tttatttgaa gctgttggaa
    13741 aaatagagaa agagcatgaa gaaagatact taaagctttt agaaaactta aatgaaggaa
    13801 aaatatttaa aagagatgaa gaagttgttt ggcaatgtca aaactgtggt catgtgtatg
    13861 taggtactga agcaccagaa aaatgcccag tatgtgacca tccaaaagca tatttcaata
    13921 taaaagctga aaattattaa aaattatcat tattaaaaag gaatgttaac tatgaaattt
    13981 tctaaacaac gagaactgat tttaaatgaa atattaaata atccggttca tcctactgcg
    14041 gattacctat atgaaaactt aaaaaaagat aatccaaatt taagcttagg aactgtgtat
    14101 agaaatttag ctcagctaac ggaacatggc tttataagaa aggttagtat tccagggtat
    14161 ccagatagat ttgatggtcg aatagataat cactatcata tcatatgtga agtatgtgga
    14221 gaggtatacg atttagaatc cgaagttctt aataatttac aagaattaat atctgaggag
    14281 acagacataa aaataacatc ttataacata agttttaaag ggatttgtaa taattgtaaa
    14341 aggtgtagcc aagtaggtta gttttcaaat tctaaaaata attaatgagg tgagtttatg
    14401 tgtagtgaac aaaaattttt tatatgtgag acttgtggaa accttgtagg aatgatacaa
    14461 agtggtggtg tacctatttt ctgttgtgga aaacctatga aggaacttgt tcctaataca
    14521 acagatgcag cagttgaaaa acatgtacct gtaattgaag tggatggaaa taatgttact
    14581 gttaaagtaa gtagcacaac tcatccaatg actaaagaac atcatatagc atgggtgtat
    14641 cttatgactg aacaaggtgg tcaacgcaaa tgtctagcag tagatggaga acctgtagtt
    14701 aaatttgctt tgaatgatga tgataaagtg atttctgcct atgcttactg taatttacat
    14761 ggattatgga aggctgaact gtaatattta aataaattga gtcaaaaagg aggctataaa
    14821 tgtattttca tacaaatagc ctcctataaa tttatataaa ttcaagtttt aattttgttt
    14881 aatgtcaata taatataagg agaaaataat atgtcaattt ataaatgtag tgtttgtgga
    14941 tatatttatg atgaatctaa gaatgataaa acatgggatg aattaagtga agattgggaa
    15001 tgccctgtat gtacaaaagg tcgtagttat tttggaaaga taagtactgt ttactatgaa
    15061 gaagatgaaa agatagcaga agacatagtt gaagatgaat ctaaattaaa tactgaaaaa
    15121 gagggagatt taaattactt aagtacatat ttaagaagag atgatgaagt tgagaaacat
    15181 atggatataa ttcacgaaat ggctgttaca ggaaaatcta taatagaacc tatgagaact
    15241 aaactaccag taatttcttg ggatgatatt ttaattatgg gtgctcaatt aaatccatta
    15301 cctttaaatg agcatgatga agtaaataca acaacaataa taggaaaaaa agcaaagaaa
    15361 cctatgatta tagaaaatcc agtttatatt tcccacatgt catttggagc tttgtctaaa
    15421 gaactaaaaa tagctttagc aaagggggca gcccaaaata agacagctat gtgtagtggt
    15481 gaaggaggta tattaccaga agagaaggaa gcttcataca agtatatttt tgagtatgta
    15541 ccaaataaat atagtgtaac tgaagaaaat cttaaaaatt ctgatgctat agagataaaa
    15601 attggtcaag gaacaaaacc tggtatggga ggtcatcttc ctggcgaaaa agtaactgaa
    15661 gaaatagcaa aagtaagaaa taagcctgta ggtcaagatg taataagtcc ttcttgtttt
    15721 gaggaaatac aatccaaaga ggatttaaag aaacttgtag atgaacttag agaagtttca
    15781 gaagggcgtc caataggtgt taaaatttca gcaggtcata ttgaaaaaga tatggaattt
    15841 atagcttatg ctaaacctga ttttgtaact attgatggtc gtggtggagc tactggagca
    15901 agtccaaagt tgcttaaaga tgcaacttca attccaacaa tatttgctct ttatcgtgct
    15961 agaaaataca tagatacaca tggattagat atagatttgg ttataacagg tgggcttcgt
    16021 atatctactg attttgctaa ggcaattgct atgggggctg atgcagttgc tattgcaagt
    16081 tctgctctta tggcagcggc gtgtcaacaa tatcgtatat gtggttctgg taagtgtcct
    16141 gtaggagtag ctactcagga tgaggaattg agaaaaagat tacatataga aaatagtgct
    16201 aatagagtgg caaacttttt aaatgtaagt ttagaagaac ttaagacttt tgctagaatt
    16261 tctggtcata aagatataca tgatttaagt gttgatgatt tatatactgt taactctgaa
    16321 atatcaaatt atacaaacat acagcatgta tagattgaag tttgaaatta ttagatttta
    16381 gacagctata aaagtaaatg ataaatatat tttataaaag agagtgtctt aaaatgaact
    16441 ttttagttca taagtcactc ttttttgtat gaagccaaat atatttatca ttttagcaat
    16501 gaaaaagaat cttatctctt tattttgaga tagactcctt ctatttatga aaattaaaga
    16561 tattaaattg taaaatattc tgtgatgtcg tactattaaa ctaaagattc tgtactttaa
    16621 agtaaataaa aatttaagga gtgattatta tggaaagatt gaagatacaa gttttagttg
    16681 acaataatac ttatattgat agatactttg ttggagaacc agcagtaagc tattatattg
    16741 agattgatgg aaataggatt ttgtttgact cagggtattc agatgtattc atatcaaacg
    16801 ctgaaaaatt aaatatagat ttaggaaatt taactcatgt tgttttttct catgggcata
    16861 atgaccatac aagaggtatc cagtttttag aaaataggta tgatttatct acagttgaac
    16921 ttatttcaca tcctaactgt tttataccta gaaaacatgg agagaaaagc ataggagctc
    16981 cattttctgc tgaggaaatt aaaaatattt ttatgtataa tcctaaggat aagccattta
    17041 atctaagcaa gaattgtgtg tttttaggtg aaataccttc tataaatgat tttgaaaaaa
    17101 gagctaaaat tggtaaatgt aaaataggag atttgtggga agatgattat gttttagatg
    17161 attctgctat agtttgtaaa actgataaag gtatttttat tgttacagga tgttctcata
    17221 gtggaatttg taatatagta gagtatgcta aaaaggtatg tggagatgat agagttattg
    17281 gtatactagg tggttttcat ctttttgaat tgaataatag gttagatagt acaatacaat
    17341 atttggataa agaagaaata agaatgttgt acccttgtca ctgtgtttct tttaaggtaa
    17401 aagcgaagat gaatgaaata ttgaatataa atgaagttgg agtagggctg accataagta
    17461 tatagtattt gaaagtatat aagat
//
LOCUS       DAEMIK010000087        17428 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEMIK010000087 DAEMIK010000000
VERSION     DAEMIK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.087"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            175..1302
                     /locus_tag="KPK87_002395"
     CDS             175..1302
                     /locus_tag="KPK87_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2538539.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            1406..2041
                     /gene="lexA"
                     /locus_tag="KPK87_002396"
     CDS             1406..2041
                     /gene="lexA"
                     /locus_tag="KPK87_002396"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG2538540.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(2188..3345)
                     /locus_tag="KPK87_002397"
     CDS             complement(2188..3345)
                     /locus_tag="KPK87_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2538541.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(3673..4305)
                     /locus_tag="KPK87_002398"
     CDS             complement(3673..4305)
                     /locus_tag="KPK87_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2538542.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            complement(4298..5656)
                     /locus_tag="KPK87_002399"
     CDS             complement(4298..5656)
                     /locus_tag="KPK87_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538543.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            5826..6578
                     /locus_tag="KPK87_002400"
     CDS             5826..6578
                     /locus_tag="KPK87_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538544.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEAISISDKVAILSKRPATIKNIYDIELN
                     MDGTKTPLKTRKVPDFQKYFDILWKELDTHEA"
     gene            6568..7368
                     /locus_tag="KPK87_002401"
     CDS             6568..7368
                     /locus_tag="KPK87_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538545.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7802..8338)
                     /locus_tag="KPK87_002402"
     CDS             complement(7802..8338)
                     /locus_tag="KPK87_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG2538546.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(8743..9252)
                     /locus_tag="KPK87_002403"
     CDS             complement(8743..9252)
                     /locus_tag="KPK87_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538547.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            9637..9840
                     /locus_tag="KPK87_002404"
     CDS             9637..9840
                     /locus_tag="KPK87_002404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538548.1"
                     /translation="MQHVINLSTIYILYKKIFEGRLKMGILEGAAMIREIAIKIAKEK
                     GITEQKAWPEAVKEFKEKYELVL"
     gene            10074..10511
                     /locus_tag="KPK87_002405"
     CDS             10074..10511
                     /locus_tag="KPK87_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG2538549.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            10650..11438
                     /locus_tag="KPK87_002406"
     CDS             10650..11438
                     /locus_tag="KPK87_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2538550.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            11483..11941
                     /locus_tag="KPK87_002407"
     CDS             11483..11941
                     /locus_tag="KPK87_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538551.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(12043..12393)
                     /locus_tag="KPK87_002408"
     CDS             complement(12043..12393)
                     /locus_tag="KPK87_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538552.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(12395..12778)
                     /locus_tag="KPK87_002409"
     CDS             complement(12395..12778)
                     /locus_tag="KPK87_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2538553.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            complement(13283..13666)
                     /locus_tag="KPK87_002410"
     CDS             complement(13283..13666)
                     /locus_tag="KPK87_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538554.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            14015..14506
                     /locus_tag="KPK87_002411"
     CDS             14015..14506
                     /locus_tag="KPK87_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG2538555.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            complement(14649..15383)
                     /locus_tag="KPK87_002412"
     CDS             complement(14649..15383)
                     /locus_tag="KPK87_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2538556.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            15769..16818
                     /locus_tag="KPK87_002413"
     CDS             15769..16818
                     /locus_tag="KPK87_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2538557.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(16968..17315)
                     /locus_tag="KPK87_002414"
     CDS             complement(16968..17315)
                     /locus_tag="KPK87_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2538558.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT         6289 a         2399 c         2359 g         6381 t
ORIGIN      
        1 attcaactag tttttaaatg tggatttagg gaatagttag tgataatttc atctaaagca
       61 ctgctaatct cttaaaaatg taaaaaatga ttaaattaaa tatttatact cacttggtat
      121 aaatatcaaa aaataatata aaagatttat attttagttt taggaggttt aattttgaat
      181 aatgataaga aaattataaa agtacataat aaatcagata aacctgataa tattgttatc
      241 aaagaaaatg aactcataga aaaatgtcta cttttggaaa ataaacttcc tgtatttatg
      301 aaggattatt ttatatattt aaaaggttct gttgcagttt ctacacgact tgcttattta
      361 gaagatatta attttttttg ttcatatttg attgaaacca aagaattaac aaatgcagat
      421 tgtgtaaaag acattacaga agatgatttt aatacaataa aatcaagaga tataaattta
      481 ttcttaggtg attattgcag tagatattac aaaaatacag aaaaaaacac attgattttt
      541 gaaaataaca atagagcttt agcaagaaaa aaatcatcca tctctactct atttaaattc
      601 ttatacagaa actctcagat agataataat ataactgatg gattcaatcc aataaaactt
      661 cctaaacctc agccagatgc tattaagcgc cttgagatag atgaagttgc taagatgctt
      721 gaatcagtag aaacaggtga agggcttaca gagaaagaaa aggtatattg gagaaaaact
      781 aaattacgtg acaaagctat actagcatta tttgttacat acgggcttag attaaatgag
      841 cttagagaac tcaatatctc ctcttttaat ttttcaagag gtgaatttaa aatatataga
      901 aaacgtggga aagaagtttt aatgcctata aatcatactt gtgaacatgt aataaaagat
      961 tatttgcaaa acgaaagaac aagagatgaa ctattaaatg atgaagaaaa agatgcttta
     1021 tttttgtctc ttcaaaataa gagaattaca gcaaaggcta taagaacact ggtaaaaaaa
     1081 tatacatcta ttccacttga tactactaga gacaatggat atagtcccca taaattaaga
     1141 gcaacggctg caacttctct aatccaaaca ggattttcta tatatgatgt tcaaaactta
     1201 cttgaccatg ataatgtcac aactactcag ctttatgcag ctcataagaa aaatgtaaaa
     1261 cgtgatatag tcaagaattt tgaatggatt gaggacgatt aaaaatcagg aacaaatgtt
     1321 tgcagatata aatggattat gttataatta aaaatataaa gtgtatggaa ataaaaacaa
     1381 aatatgtttg gaaatgagtg atgttatgta tttagatcta actgaaaagc aagtcttgat
     1441 attggaattt ataaagtctc aaatcatatt aaaaggttat ccacctgctg taagagaaat
     1501 atgtactgct gtagggttaa gatctacttc aactgtgcac tctcatctaa ataaacttga
     1561 aaaactggga tacataagaa aggaccctac taaaccaaga gctattgaag ttttagaacg
     1621 tagtaaagta aatgatgttt ctggagctaa tcaagaaata atagagcttc ctttagtagg
     1681 tcaaataaca gctggagaac ccattttagc tcaacaaaac atagaggaat acattccatt
     1741 ccctgccagt ttagtaaagg gcagtaacaa ttttgtatta agagtaaaag gcgaaagtat
     1801 gattaatgca ggaattttag atgaagatta cgttgtagta gacaaaaaaa atacagcctt
     1861 aaattctcag atagtagttg cacttataaa tggtgaatct gctacagtta agaggttttt
     1921 taaagaagga aatttaataa gacttcagcc agaaaatgat tttatggaac caattatgct
     1981 caatgactca gaagttgaaa ttgtaggtat tgtaactggt gtatttagag ttattaaata
     2041 gctattaaaa aatacttaaa aaaataagct attcgtatag ataaaatgtg ttatctattg
     2101 attatagctt atttttttat gcatttattt tattttttaa atgatttaaa gttcagaagc
     2161 tttatagata aattggttgt ttttttgtta ttttccataa catatatcac atgggtcatt
     2221 tttcttactt tctgcaattg tgcctgataa tattttttta cttcttgaaa gcccactaca
     2281 attttttgta gagtgataac tcttaccttt tggagtaaaa tacactattt cttctgaatt
     2341 ggatgtattt tgtttatttt taaaagaatc atttttactt gtattactgt ttgattttcc
     2401 agagtttcca tcttctctaa cgcctttatt ataacttcca tctttacaac ttgtaaatac
     2461 tccatcacct gttgattcaa atattatatc tgaacactca tcacttctat gtacttttac
     2521 tcccattttt tttaatttat tcattgtctc ctgatgtgga tgaccatatg aattaccctt
     2581 gcctactaaa agaaccgcat attcaggatt tactttatct aaaaactctt gagatgaact
     2641 actacgagaa ccatgatgag ctacttttaa aaggtctact ttctcaagct taggtagtat
     2701 ttctttttcg gtaccttctt cagcatcacc tgtaaataaa actttatcat tcccattagt
     2761 atatacaagg actagagatt gttcattagt agtatctccc ccatttgtat ttagaacttc
     2821 aaaataagaa tcttctaaat agaacttatt attttcaagc ggtacacttg gggataaacc
     2881 cttatttgct aaagcattta taaagtccct ataactcttt gtttctgctg aaccattcga
     2941 tacaaaaaca ttttctatat taaagttttt gacaacagaa tcaagtccac ctaaatggtc
     3001 tgcatgagaa tgggtagcta taagatattt aatatctttt acaccttgat tgtttaagta
     3061 atctaccatt aatccctcat catcattatc tcccccatct atcaatgtaa aagttttacc
     3121 atcttgaatc aatattgcat cactatttcc agtgtcaata aaatgaattt ttacattctt
     3181 atcacttgat atactactag acaaagaatc attattattt gattgattat ttccattatc
     3241 tgatgtagta caaccagtta aataaatcga tatagatact atcaataaaa gtatcaaagt
     3301 tttaatttta gaattttttt tattttttat acttatttct atcacaaact acgcctccat
     3361 attcataata ctctagtaat tataacatga tatgcgtttt tatgatattt ttttacataa
     3421 tattttaaat aatagattat agtacaataa tatagaaaat tgatactgta taatttttat
     3481 attatataaa atgaaatatt ttacatcagt agtatccagt tatgcatttt aaacgaccat
     3541 ttacgtttaa tatattgcaa actgcttcta aaaactataa tatgttaagt atcaacaaaa
     3601 ataaataaac tttttcctta aagaaggctt aaagctcaca ccaactgcct tctttttttt
     3661 ataaaaattt aattatttat tttgattaat atttatttcg tattttagtt ctactcttct
     3721 agatgcatct ctattcacgc tattgtcacc atttttgatg agttcagctt ttgaccttcc
     3781 tattgcaata atatgcttag tcactttatc tttgtattta tagtcaccta tttcatctcc
     3841 cactataaat ttagcaacgc tatatgctct tctttgcgat aaatctaagt tgtatatata
     3901 acttcccaca tcatctgtat gaccttctat aactattttg gatatatagt caccataatc
     3961 tttataaata gtctctatgt actttggaac aaacattttt agcatttctt tagcctctgg
     4021 ttttaaaaca tcactatcta catcaaacat ggttttttca ccaaaagtta cttgcccatt
     4081 agatttatct accttcacag gtattccact ttttttaaaa gtttcttcaa cagaagcatt
     4141 gacattctta gctaaatctt gttcaacaga acctgaaata ctttctgagc taaataagat
     4201 aagaatcaca accatcaata cagtagataa taaatctgta aatgttggcc aaaagctact
     4261 cttttcaaat tctctgttaa cagttcttct atatttatta ttcatttaac tcatcctcac
     4321 ctatgacctt tatttcttta tcctctatct cataaatttc ttcactgaaa ggattaaata
     4381 cattattaac atcatcctta aataattctt cactatcaag aggaacttca tcattcataa
     4441 aagagttatt aaacataaca tctaacttat cttgaatatc taaagaattt tcaaacatca
     4501 tatccagttt aacttgtagg tcattagaaa agttttcaat aatacttgag agatttttta
     4561 ttgagtcatc taaggtaaac ttttcatcta aaaggatttt ttgattttga gatattttat
     4621 tttcgtgggt cattaattta gttaataaat cttgagcact tctttcatat agagcaaatc
     4681 ctgcatttat attttttata taatctctaa ttttgaattg gctttcagtg gcattgtata
     4741 tattttttga agattctttt atatcaaata aaatctcttt atttatatta tcttgattat
     4801 catatttatc aaaaagcttt acaaatttta tatttaaaat attaaactgt ttatctaatt
     4861 ttcttacgct ctggtcaaat gtcttaatag aactttgaga accttctaaa aggcttttta
     4921 aagcttctat accactgata aattcatcat taaagtcatt taaatcttta gatattttat
     4981 caaatttttc aatagcttct atagacttac ttatttgttt tattgtattt tttacctctt
     5041 ctacttttaa atccattctt tctgacttaa aatgagttat ttcagatgtt agtaaatttt
     5101 cagattttaa cattatttgt atcaaaacat gttcacaatc ttttatctta gtaaagtaac
     5161 ctataattag agaaaataca actccaaata tactagttgt aaatgcagtt tgcatactac
     5221 taatagttga aggaagtgaa tttataatgt ctttagtatc aacacataaa agcattgtag
     5281 aaagaccaac aaacgtacct aaaaccccaa gtaatatact tattgaagat gaatttttta
     5341 tacttctaat tttttctaac aatggcaaat tattatgctt taaatcagat acaacttcct
     5401 ctataaatga agttatattt acgtctgcat aaggattttc tttactatat gtatagtagt
     5461 catcccttac ctttttataa aactcacttg tcttataatc tctattgatt tcgtctaatt
     5521 ttgaacttac aaatgaatat actctgttat tttctattat tttaaaaaca gaatacacag
     5581 ttattgcaag taaaaacaat atagttattg gattagtaaa tattgactct attaaattcg
     5641 ttattatgtt actcaaattt atcccctacc ttataattta tttatctata atttatacgt
     5701 acaagttact aagaaaatac gtaaataaac aaaatattta tattatattt atgaaaaatg
     5761 gtacaaatag gtcatattgt taataacata atatatacga gctaaaagtt atagggggtg
     5821 gcataatgaa tgacaacata gtagaagtta aaaatataaa tatgaacttc tacactaagg
     5881 aaggcgagct tgaagtatta aaggatgtca attttaactt gaaagaagga gaaatattaa
     5941 ctttgcttgg tccttctgga agtggtaaat ctacaatatt aaatattctt actaatcttt
     6001 taaaaccgac tagtggtgat ataaagatta ctggaaatat tggatatatg tttcaaaaag
     6061 ataatttgct tgaatggaga aatataatgg ataatataac tattggattg gaaattcaag
     6121 gcaaaaaaga taaaaaatct ttagatagag ttgaaagcct tttaaagact tatggattat
     6181 gggattttag aagtatgtat ccaaaagaat tatctggagg tatgagacaa agagttgcgc
     6241 ttattagaac actttctgta aatccagata ttctactttt agatgaacct ttttcagctc
     6301 ttgattatca gactagatta ttagtttgtg atgatgttta tagtattatt aagaatgaga
     6361 ataagtctac tattttggtt acacatgata ttggtgaagc aataagcata tcagataagg
     6421 ttgcaatact atctaaaaga ccagctacca taaaaaatat ttacgatatt gaacttaaca
     6481 tggatggaac aaaaacgcct ctcaaaacac gtaaagttcc tgattttcaa aagtatttcg
     6541 acatattatg gaaggagcta gatacacatg aggcttaata aaagatctga aggatatata
     6601 aactatgtta aaggtgtaaa aagagaaaaa agaaatgtat tattctacca gctattaatt
     6661 ttaattggtt ttattgtaat ctgggaactt ttagctgact taaatgttat aaatacattt
     6721 ctatttagca aaccaagtga tatttacaat ttatttattc aatatgcatt tagtggtcaa
     6781 ttatttaaac atattggaat atctgtatat gaaactgttc ttggtcttgt tataggtact
     6841 gtattaggta ttttggtagc aattgctctt tggtggtcag aaaaattatc aaaaatatta
     6901 gacccatttt tggtagtttt aaatgctctt cctaagactg ctttagctcc tataattata
     6961 gtttgggtag gtgctggaat agaaggaatc gtcgtaacag ctgtaacgat ttctgttgtt
     7021 gttactatac tttcagctta taattatttt atgaatatag atgaagaaaa aattaagatg
     7081 ttaaaaagct ttggggctac taaatctcaa atattattta aactcatatt acctgcaaac
     7141 acaggaaatt taataaattt aactaaaatt aatattggta tggcatgggt aggtgtaata
     7201 gttggagaat ttttagtttc aagatatgga ataggctact tgatagtata tggttctcaa
     7261 gtatttaaat tagatttagt aatgatggga gtatttgtat tggctatctg tgcttgggca
     7321 atgtacgctg tagttaatat tatagaaaaa atatacaatt ccagataaat aaaaatttta
     7381 tttagaatat ttgatgtatc agtgattata ataaaaaatg atttctttac agtaatcatt
     7441 tttttaaatt aaaataaagc gtaagttttt gattatcttc ttataactca tcatgttgct
     7501 agcacagctc tattttatgg gctttgctgg ctttttattg cctccaatta tatacataat
     7561 gcttaaatac agtcatttct ttattaccta tgcttaaatt tctacaaaag aaaagacagt
     7621 caatttttta atcaaaatta actgtgcttt atatttaatt tttaattttt atttttgtgc
     7681 tccgtttgtt agatatcaga gacttaatta gtgctcacag tttagcccaa aagcttaagt
     7741 aagccaacta tttgctgtga ttaaataagt ttaccttgaa acttaaattt atcttctaaa
     7801 tttatttttt aaactgacga tagcaaaaat atatgtattc tttcattgat atttctctat
     7861 tatgcaaaat tcctatgaca acaaatatta tttctgcaac agtaaggtag cagtctttta
     7921 agtcgaaaat aaaaaagctt ggtatctgca aaaaatctaa acttcctccc caaaataatt
     7981 tatctatcaa gctacatatt gtaccagcaa gtccacaaga cataattact tttactgaat
     8041 agcttgtttg ttcatttttt gatttataaa aagcatatcc agatataata actaatataa
     8101 ctaaaatgtt aaacaatacc aatacccata aattagatag aataccaata aagtttcccc
     8161 cataagagag atttgtattt tgaacaggat tgaatcttaa aaatttccca attatatcaa
     8221 tttcaaagtc cattaagaat aatcctataa gaactttgct aatttggtct aagaatatta
     8281 aggatataac tggaaaaaca aaagacttta cttgcctgat attaacacct ccttgcaaaa
     8341 gtcattcact ttttccttac tcagtattgt taaatccaaa aaaggaattt cgatttatta
     8401 atttcacatc aataattata acatgagtaa attatcaagt catttctttt ctgatttcgt
     8461 agataatact tatctccata tatttctact cgtagtcgtt gtacaaatcg ttgtaaatca
     8521 tgctaccaga cctcgcattt cctgcatctt acgcatgaga gtataagtcc agcagtcttg
     8581 cttcctgtac tttacttaat gcggttttgg gcctactgtc gtcctcaata acttcgctta
     8641 gttggaaatc caaaaggttc ttccttacat agtcgtcctc gattgccatg tagaatgacg
     8701 cttttaagga acgcttgctg tattataaaa agcagagtta cttcatattt tcttacgtgt
     8761 caaaaagatt ccaataattg ctcctataag gacaatcggt actaatctga caaatagcca
     8821 ctcaaaagca tgaaaagcta ctccaaaaac agcggtttca gggtcggtat aattccaatc
     8881 caagtaagga ctatttagtc tccctaagac cacaaaaact attaatatga ctgcacataa
     8941 taaaataaat aaccaatgaa acatttttct tctcatgttt tttctttgtg caagttgcaa
     9001 gtttataatc tctaattttt cagaaatcac ttttaagtta tcaacttccg actcaacaat
     9061 agtttctcca agtaaagtgc ttacgggcgt ttcaagcacc tctgatatgg aaatcaacat
     9121 atcagaatcg ggaaccgaca gtccttgttc ccatttagat attgtttgtc gcactacatt
     9181 cagttttata gcaagttctt gttgtgaaag tccttttgac ttcctgattg ttttaatgtt
     9241 ttcgcttaac attagggcta accacctttc tttcagttgt taccgatatt ataggcaaaa
     9301 ctgcccattg cttcaagcaa cgcattttaa cattcaagta aatttcttct ttttccttta
     9361 accgttttgc ttatatacag tttttatgcg gatattctcg atttttaagc gctttttata
     9421 ataaaaatag atatgtctac taattaaaac tgtagaaata tttataaagg cataagacaa
     9481 atttacctac aatttttagt gtttagattc taaaaaaata aaaataatta taattgagtt
     9541 cataatttta aaacttattg aatataatta tatgtcggtt aaagacgtat gaaaataaaa
     9601 ggaatttaca agttatgtgt tgtatagata ttgtatatgc agcatgtaat aaatcttagc
     9661 actatttata ttctatacaa aaagatattt gaagggagat taaaaatggg aatattagaa
     9721 ggcgctgcaa tgataagaga gattgcaatt aaaatagcta aagaaaaagg tattacagaa
     9781 caaaaggcat ggcccgaagc tgtaaaagaa tttaaagaaa aatatgaact tgtattataa
     9841 actttgactg taaaactgtc tctattttat tgagacagtt ttttatattg taacattcat
     9901 attacgaaga aagatatata taattttaga ttccttgtaa ctttaattta aaatgaaata
     9961 atttagacta aaaacagtat taataaattt tatttatcgt ataaatttgt ttttaatatt
    10021 atttttaagg tataaatctt atatattaga tataaatttt ggaggtatat gatttgaata
    10081 aaaaacttga aaatagatgc tcatggcaag aatattttat gagactgtgc gaaacagtag
    10141 ctgaaagagg aacttgtgat agagcttatg taggagctat aattgtcaat tcagaaaaca
    10201 gaatagtgtc tactggatat aatggtagta tttctggaga taagcattgt agtgaagttg
    10261 gtcatgaaat gagagatgga cattgtatta gaaccattca cgctgaacaa aatgcccttt
    10321 attattgtgc aaaagaaggt atttcagtta aagattgctc tatttatgta actcactttc
    10381 cttgcttaaa ttgtactaag gctataattc aagctggaat taaacatata tattatagaa
    10441 ctggatatag aatagatgaa tatgctataa aattattgca atcaagtaat gtcttatata
    10501 caaaattata attttaacat tatttactta atattctata tttttacaaa atgggttata
    10561 ataaaagtat aagatataaa aagaaacaaa attctaggtt atgcatatat taaatagaag
    10621 ttaaatttaa ttgtaaagga gatgttagtt tgaaagtagt aataatacca gggcacactt
    10681 taattggaaa aggtacagga gctgttgggt atataaacga aagcaaagaa acaagaatac
    10741 tgaatgactt aattgttaaa tggcttaaaa taggtggagc tactgtttat acaggaagag
    10801 ttgatgaatc aagtaatcat ttagcagacc aatgtgctat agcaaataaa caggaaactg
    10861 atttggctgt tcagattcat tttaattcaa atgctacaac ttcaactcca gtagggacag
    10921 aaacaatata taagactaat aatggcaaaa cctatgcaga aagagtgaat actaggttag
    10981 ctacagtatt caaagatagg ggtgcaaagt ctgatgttag aggtctttat tggcttaatc
    11041 atactattgc accagcaata ttaatagaag tatgttttgt agatagtaaa gcagatacag
    11101 attattatgt taataataaa gataaagttg ctaaattgat agcagaaggt atactaaaca
    11161 aatctatatc aaattctcaa gggggagggg aaaataaagt gtatgaaaat gtaatcgttt
    11221 atacaggtga tgctgataaa gtggctgctc agattttaca ttggcaattg aaagattctt
    11281 taataattga ggcgtctagt tataagcaag gtttaggtaa aaaggtttat gttgtaggtg
    11341 gagaagctaa taaacttgta aaaggtgatg tagtaataaa tggtgctgat agatatgaaa
    11401 ctgttaaatt agcattacaa gaaatagata aattataaaa tcatgattat tttataaaaa
    11461 ataaataagt taggataaaa aaatgataag aacaaaagtt gtagagttga tagcaacagt
    11521 atgcagagaa aacaaaccac ataaatgggt tgatgaaaat tatactcctt atgataaaag
    11581 tggtaaagtt gaattaatgt caatagaaga tttaaatgaa ttaatatcaa gcagtggaag
    11641 agcagatttt ctttatagca gtagattaca aaagcttcta aatgaggttt atataaatca
    11701 aagtagagca tcttatatat caggttgtgg attattttgg agttcgtatt gggatatact
    11761 tgaagaaaaa tttgaagaat ggctatataa ttcttatatc ttttttgatg aagatgatga
    11821 atatttagaa ggaatggaag attttgagct tgaatgtaaa gatgttctta tggatgtaat
    11881 tgaaacaaca agcatagata tatatgttca aatgataaag aggaatatta ccaattatta
    11941 aaaacacata gtgtcaaatc aagctaataa aaaacgacct tataaatgct attttaaggt
    12001 cgtttaaatt atatcttata ggcttatttt atattaaatt tattactttt tatctagaaa
    12061 aatattttta attatagtca atatatcgac tataacatcc atatttgatt tgacactgtc
    12121 tttagtaaca agaaaatctg ctgtaacttc agtaactact tctgttacat ctttcatatt
    12181 ttctcctact tctccaaagg tttctacaac attaggaaga ttatttaaag ttgtatttat
    12241 attttgagta ttttcattta acatagttcc aacattattt aaaatcttat gtagtttaac
    12301 aagtacaata attaaataaa ctaaagcaat acaagcaagt atagctaata atacatatat
    12361 tatacttatg ttaatcgtta ttgtattgtc catattattc ctcagtaaca ctaactggtt
    12421 caactatatc tattatttta tcttcctcac tagaattttc aacactcttt ttgttatcta
    12481 tatatttttt tatttttttc tttgattcta taaagtttcc ttttttatcc tctagcttct
    12541 cagatacttt gccttttaca tctacagttt tcatatttat cttttcagca atttgttgtg
    12601 aagcatcttt tatatcctct cttgtttctt ttccagattt aggagccaaa agtactccac
    12661 tcactgcacc agtaacagct cctaatacca caccagctgt agcaatttta gctttcttaa
    12721 ctttttcagc tctctgttta gcttttcttt tatcttctat tttcttcgat agattcatgt
    12781 ttaatcgctc cttattataa tatttctaaa cattataata cattttttac cataatgata
    12841 tagtttctaa ctaaattttt aaaaatcaat aaaaattctt tttaattact tcctattcat
    12901 tacaatttaa atttttataa ggattttcta tatatttttc ttttattaat atataatatg
    12961 tatcacatat agattataaa tatcttattt aattagttta atgtatataa ctgaaaactt
    13021 ttagtataaa tgaccttcaa tctttctata tagattaaat tagtatctca aaaaacaata
    13081 agagaggaat taatcctctc tttttaattt aattagattt ggcagggcgg cgtatcctgc
    13141 atctctatta actactctat agtagcgtat tgggtgcctt ttccaacctc aaatctaact
    13201 aataaatatc tacgtgtata ttaatattat aacttatata ataatatttt tcaatatttt
    13261 agggaaaatt aataatatta tgttaaccat catattgttt tactcttcca ctgtttactc
    13321 tactttctaa ttttgcttct tttatagcat acattgaaga tacatataaa tatccttttt
    13381 ttgtatctaa ttctgtagct actaaaatat aatcatcaat tttttttata aactccacac
    13441 tttttccagg ttttttagga tgacttccta tatattcagg tgactttaat accattttta
    13501 tagtatctat tatatcatta aaaatatcct tactcagttg acatctatgt tttttcttta
    13561 tatgttttat aacaccagaa gaagcataaa ttttaccagt atattcaatt cctgcaagtt
    13621 ctgccgtttc ttcatctatg attccaacaa ctttgtctgt agacatataa agcacattcc
    13681 tttctaagat aatttactat taatactgta aaatttattt aaattaaata tctatattta
    13741 aagtatagtt tattccaaaa agaaaagata gtataatata gttttttcat atatgacttt
    13801 atataatccc tttttatata atttataaat ggcaaataag gttctatttt caaggtattt
    13861 ttagaaattg tttaatttct ttaagtttta ttgagtattc ttgcttcttt ataaaaatat
    13921 aatctattgt ctatattata taaaacgttt tcttttaaag ttttctgtga taatattatt
    13981 tatatataaa ataaatttct aataggagga aatcatgaac aatcaacaac aggaagtata
    14041 taaaaaatta gatgagttaa gcatacctta tgaggctgta aatcatccac cagtttatac
    14101 aattgaagaa atggaagaat taaaaacatt acatatggaa tttgttgtaa aaaacctatt
    14161 tcttcgtgat gctaaaggaa aagaacattt tttagttgta ttaggtaaag acaaaaaagc
    14221 agatttaaaa gatattcgtc aacaaattga tagtaaacct cttagttttg catcagaaga
    14281 aagacttcaa aaatacttga aacttactaa aggagctgtg actccttttg gtatattaaa
    14341 tgataaagag gctgttgtaa aagttgtatt tgataaggat ttattaaaaa tggataaaat
    14401 aggtatacat ccaaatgata atactgctac tgtattttta aaatttgaag atatgaaaaa
    14461 gcttatagaa gaaaatggaa acgaaatata ctacgtagaa gtataattgc aacttaaaaa
    14521 atggcaaaag caacgaatgc ttttgccttc ttactactgg tgtaattaat accactggta
    14581 taatcatctc cattagtgta attattttct acaacttttt ttctacaata atgattattt
    14641 atatttactt atcttttttt tgcagataca agtaacatca taggtctacg tagctcatct
    14701 ttcatatcag gaattgtatc taacagtttt tcttctggtt gaggttcaat aatttctaat
    14761 atttcaaatc ctgttttcat taacgtattt aaataagttg ttaatgtttt atgatatttt
    14821 ataacctctt ctcctagaaa atttgactta cgtactcctt cttcaaaata tctatctact
    14881 ggccagtgta gattatttcc atctttatca cagtaccact cttgacttcc atatgcagta
    14941 aatataggat gttcaacaga aaatacaaaa ttacctttat ttgaaagaca attacttata
    15001 ttcttacata tatcttcaaa tgattgaata taatgaaatg ccaaggaact aataactaca
    15061 tcaaaactat cttttggaaa attaatatct tcaataggca tacatatgta atttatatta
    15121 tcaagttgcg ttttgtttct agcttctttt aacatttttt ctgaaatatc aactccaaca
    15181 gcagaaattg ctccattctc aactgcatat tgacaatgcc atccaaatcc acatcctaaa
    15241 tctaaaactc tttttccttt gaaatcaggt aacatttttt ttaaaacatg ccactctcca
    15301 gcacctgcta atccatttgt agaacgttcc attttactgt atttattaaa aaatctatca
    15361 tcatcatatt tattttgttt cataaagttt cctccatttt aaatatattc acttagcaac
    15421 actctattaa atacaaaata taagttttta tcacttttat ataaaataat aaaagtttct
    15481 ataaacaaat tttttaataa tataaatgaa tatgatttat attattaaaa aatttttcct
    15541 tatatataat agacaaaaaa gtgatattat tttaatttta ttacttagtt tataaataat
    15601 caatataatt tattcaatat tacacattac tgaaaaaact aaacataaaa ctgaaaatta
    15661 ttattaagtt tttgtagaaa tattataagt atagatacat ttttgtaaca actacaataa
    15721 atatttaata ctatatacta ttcgtatgtc ttatgggagg tatttattat gtttaaaaaa
    15781 atagtcgcaa tagcaagctc tatcattgta agtacaatat gtctaactgg atgtgatttt
    15841 aactctaatg acaaaaaaga tgaatcttct tcgtctaaaa atctggataa agtaactata
    15901 gcagaagtta ctcactctgt tttttatgct ccacagtatg ctgctattac gaaaggattc
    15961 tttgaagatg aaggtataaa gattgacctt ataaataccc aaggtgcaga taaaactatg
    16021 gcagctttga tttcaggtga agcaaacgta ggtttaatgg gtcctgaagc atctatatat
    16081 gtatataatc aaaacaaaaa cgattatgca gtgaactttg ctcaattgac aaaaagagat
    16141 ggtagttttc tagttgctag gaaaaaaatg cctaatttct cttacaatga tttaaaagga
    16201 accgaaatat taggtggtag aaaaggtgga gttccactaa tgacttttga atatgttcta
    16261 aaagaaaaag gtcttactat tggagaaaat ctaaaagcag gcaatgttaa tgtaagaact
    16321 gatgtacaat ttggtgtaat ggctggagct tttgcaggtg gagagggtga ctttacaaca
    16381 gcatttgaac ctacagcaac agcaatggaa aaagaaggta gtggatatat agttacatct
    16441 ataggaaaag attctggaga aatacctttt acagcatact ctactacgag atcatacatg
    16501 aaaaacaata aagatttaat tcaaagattt acaaacgctg tttataaagg tcaattatgg
    16561 attcaacaag catctagcaa agaagtagct aaggctatgc aacctttctt tgaagatata
    16621 tctttagaag atttaacaac agttgtagat agatataaat caatagatgc atggtctcaa
    16681 aatcctgtat taaaagagga aggtttgaag aaattgatga ctgttatgaa agaagctaaa
    16741 gaactagata aggaagcacc ttataaagat atcgtaaata ctgagtttgc aaacaatgct
    16801 ataaaaaata ttaaataaaa tatattagtt ttagctagat attttcttgt ttttataatg
    16861 aaaattaaca aaaatataaa aagagccttt taaaagactc tttttatatt tgagattaaa
    16921 gtttatataa taaaatagat attaaagtac taactgtact tatatatcta tcctctgtat
    16981 tctgctccag cattaatttt tgtaatttga acggctacca tttcttcatt gcttccatta
    17041 gtaatttcat gccactctcc tgttggtaca aatacagtat cgccgttttt tacatcacat
    17101 tcatgctcac caagtttcat tttaccttct ccttttagga acacaaatat ttgttcacca
    17161 ttagggtgtc tatgagcatt aagaacttga ttaggtttaa aataataaac ttctgaagtt
    17221 atatcgttac cagcaaatac tggtattttt ggaactgctg tatcattata ttgagctaag
    17281 cttccgtcta atagattctt tttattttca tgcatactag aaatacctct tttctatatt
    17341 atagatttac tattattata ccaatataag aaaatagtat tctcacttta cacaatagga
    17401 ataaaattct tttattttta aatataag
//
LOCUS       DAEMIK010000088        17232 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEMIK010000088 DAEMIK010000000
VERSION     DAEMIK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.088"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..911
                     /locus_tag="KPK87_002415"
     CDS             <1..911
                     /locus_tag="KPK87_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2538559.1"
                     /translation="YWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEYSSNINI
                     DTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQ
                     IHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDL
                     SFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAI
                     KACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQG
                     AISIDKTSNYFQINVLLVISLHCASI"
     gene            950..1435
                     /locus_tag="KPK87_002416"
     CDS             950..1435
                     /locus_tag="KPK87_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538560.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(1698..1934)
                     /locus_tag="KPK87_002417"
     CDS             complement(1698..1934)
                     /locus_tag="KPK87_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538561.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(2089..2313)
                     /locus_tag="KPK87_002418"
     CDS             complement(2089..2313)
                     /locus_tag="KPK87_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2538562.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2410..3663)
                     /locus_tag="KPK87_002419"
     CDS             complement(2410..3663)
                     /locus_tag="KPK87_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG2538563.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNDFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            3937..4926
                     /locus_tag="KPK87_002420"
     CDS             3937..4926
                     /locus_tag="KPK87_002420"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG2538564.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHEKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            4998..5738
                     /locus_tag="KPK87_002421"
     CDS             4998..5738
                     /locus_tag="KPK87_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG2538565.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            5954..7105
                     /locus_tag="KPK87_002422"
     CDS             5954..7105
                     /locus_tag="KPK87_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG2538566.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7130..7885
                     /locus_tag="KPK87_002423"
     CDS             7130..7885
                     /locus_tag="KPK87_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG2538567.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNNNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8077..10464
                     /locus_tag="KPK87_002424"
     CDS             8077..10464
                     /locus_tag="KPK87_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG2538568.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10695..10928)
                     /locus_tag="KPK87_002425"
     CDS             complement(10695..10928)
                     /locus_tag="KPK87_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG2538569.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            11217..11807
                     /locus_tag="KPK87_002426"
     CDS             11217..11807
                     /locus_tag="KPK87_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2538570.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            11921..12766
                     /locus_tag="KPK87_002427"
     CDS             11921..12766
                     /locus_tag="KPK87_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538571.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            12763..13446
                     /locus_tag="KPK87_002428"
     CDS             12763..13446
                     /locus_tag="KPK87_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538572.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            13443..14159
                     /locus_tag="KPK87_002429"
     CDS             13443..14159
                     /locus_tag="KPK87_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538573.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            14256..14519
                     /locus_tag="KPK87_002430"
     CDS             14256..14519
                     /locus_tag="KPK87_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538574.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            14491..14946
                     /gene="def"
                     /locus_tag="KPK87_002431"
     CDS             14491..14946
                     /gene="def"
                     /locus_tag="KPK87_002431"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG2538575.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEEEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            14998..15459
                     /locus_tag="KPK87_002432"
     CDS             14998..15459
                     /locus_tag="KPK87_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG2538576.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIIF"
     gene            15509..16411
                     /locus_tag="KPK87_002433"
     CDS             15509..16411
                     /locus_tag="KPK87_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2538577.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GALYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            16781..>17232
                     /locus_tag="KPK87_002434"
     CDS             16781..>17232
                     /locus_tag="KPK87_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2538578.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYNVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKI"
BASE COUNT         6870 a         1819 c         2789 g         5754 t
ORIGIN      
        1 tatattggta tagttctaaa aagtttaata ttaaggtcat gaactttgat aaatatatag
       61 caattttgat gattccattg ttgcttataa tattatttat gcaatcattt gaatatagtt
      121 caaatataaa tatagataca agtcttggga ttgtaaagct gctattaaat acttctataa
      181 ctgaagaaat tcaagctttt cttttttcta taattggaac tatatgtgta tttttttctc
      241 tatttacctt taagaaacta attcaagctc tcgaagatga caaggagagg gcaattatga
      301 atcaacagat tcatgctcaa aaaaactata ttgaagaagc gaaatcacga ttatcacaaa
      361 ctatatcctt tagacatgat tttaataatc atttagctat tgtcaatgga ttgttgaaaa
      421 aagaccaaat tttaaaagca caagattatt taaataagct tgaaaaagta acaagtgacc
      481 tatcattttc ttgtaataca cagaatattg ttattgatgc tcttttcaac aataaattaa
      541 gtatagcaaa acaattaggc atagaggtag attgtgaagt aattataccc caaagcacta
      601 acatgaacga ttttgaccta tgtatagtgt ttgctaatgc tattgacaat gcaattaaag
      661 cttgtagagt tgtagataaa caaaataggt atttaaagct ttccactaag ttagagggtg
      721 gattttttat gatagaaata aaaaatagct ataatccaat aaataattat acaagtggct
      781 caggtatagg tcttttaaat ataaaagaaa ttgctaaaaa atatcaaggg gctataagta
      841 tagataagac atcaaactat tttcaaataa atgtattgct agttatttca ttacattgcg
      901 ctagtatttg agaacaattt cattgaaaat agtatttgta agctatatta tgtacttgaa
      961 tagagaaagt ttgatatata gaaatgagga ggttactaaa atgaaagttg gaaatataaa
     1021 tggtcaagca ccaattacaa atggtttaga tagttgtcaa agtgaggtac aaggcttaga
     1081 acaaaagaaa aaagcattac aggatgaaat agagaaagta agaagcgata aaagtattga
     1141 tggtgaaacg gccaaattaa aaatagaagc tttaaaaaaa gaaatggaag agattgaaaa
     1201 taaaattcaa gagttaaaag gaaataaagg aaaaaaaaca aataaagaag aaaataacgt
     1261 ttcagaagta aataataatc tattagagag aaaaaaagaa ccttctaata atgaaaatgt
     1321 aaaacaatta tatgatgttt ttgaaagaag caatgtagac gaacaatggg ataatactta
     1381 tcgtgttctc caagatgata aaaacttaaa agtaaaattt aatagaccta tatagtttat
     1441 ttataaaaat agttaatatt ttaattatca ccacttttta gaagaataca cactttattt
     1501 taaagaccca catatttatt tacagttagc acatttatat gtggttttat aactattatt
     1561 tataagtcta tattgataac ttttacatag tatataatgt ttaaatactt attcaaattt
     1621 atctatacat aaacaaaaaa agagatttta aaatctcttt ttagattgtt gacttatggc
     1681 tttttgattt aataaaatca agaagccgtt tgaattttat ctgttggctg atacccttct
     1741 gatattgaaa caccattttt gtttccaaaa tcgtttaaaa ttaagaactt attatgaatg
     1801 actatttcat tatgaacaat aagcagataa catgaatctt caatcaatgt tttaacagta
     1861 gcattaaatg cttgcttata cagttctatc gtttgtttga actgtccatt aaagtaaata
     1921 tgagtcaata acataactat atctacacaa attaatataa attttttata ctgtacatta
     1981 tctaactgtt gattatctgt attacaacat gattgaattt aaaaagccat caacacatag
     2041 aatgttgatg gcctgaaaat agatttaaaa tctctttttt attatatatt aattattttt
     2101 taaatttata ttttttgcat cagattttct aatagctaag tcaaaccctc taaatctaat
     2161 cactataggg tcattaagag gagctatctt tttcacttca acttcagtgc catctattag
     2221 tcctaaggct agaagtctct tcataagttt ttcatttcca gctatattgt ctataattcc
     2281 cttttgtcca agttttaagt tgtaaacagt cattattaac acctcctaat gaaaatcatt
     2341 atcaaacttt atatttataa tatataacaa aaagagcaaa ttgtcaatat ataacaaaaa
     2401 ataaacatac taagttagta tgtttataaa aaatagtata catgggatac taatagcatc
     2461 tagcaaaaaa ccgcttacta atggtacaac caaaaaggct tttggagaat caccatattt
     2521 ttgtgtcaaa gaactcatat ttgccaaagc gttcggagta gctcctattc catgtccaat
     2581 caaaccactt attatgatgg cagaatcaaa atcttttcca agtactctaa agcaaataaa
     2641 tattgaaaat aggattataa agattacttg aaaagttact attaagagaa taggaggtaa
     2701 taatgtagat aattcaaata aatctatatt cataagtgat aaagttaaaa aaattcccaa
     2761 tgaaagttct tttaaaaaat ctataagttt aaaatttaat tctagtatat ttatcttatc
     2821 atttaaattt ctaaagataa cagccgaaaa catacaagtt acaacagagg gtaatagtat
     2881 attaaacgtt atatagaagc atctacttat aatttctcct gtatttatac aaataaatat
     2941 gagaagaacc tgttccaaaa acacagatgg tgttattgtt tcggagaagt tattattttt
     3001 tatcctattc ttatttctaa aaaatttact tccaaatctc tttagatttc tattatatct
     3061 aggaatcgac aagtctatat ttgatgatgg ttttaattta tatttattta ttagaaattt
     3121 agctacagga catcccaata taccacccat aataagtcct attgttgcag cagataatcc
     3181 aaccccaact gctccttcta taccgagatt ttctattgta agtccaaatg cagcagaata
     3241 accatgacca ccttccatag atacatttcc acacataagc cctaaaagag gttcaagttt
     3301 tataatcttt gatattgtaa tagctaatat attttgacaa aatgccaata ctccacacaa
     3361 aatccaatat ctaaataata cactaccccc tttctttata agatttatgc ttatttcgag
     3421 tcctatagtt gtaaaaaaga aggttataaa atttggcatt aaatttccac ttatagatat
     3481 atctgctata ttaaaatatt taaaaaataa atttagaaaa caaaataata gacctcctat
     3541 tactggtgca ggtatacaga aatcgtttaa aaagttaact ttattcttca ataaattacc
     3601 aattaaaaag aagaatatag atagtgtcaa ggtttgcact atatcaaaac ttaaggtaat
     3661 caaaataagt cctcctttgt aattcaaagt tttaattata taacttaaat tcttatgtat
     3721 tgaaatttta ttatcaaatg tatattaata aatgtatatt aataaatgta tattaaaaag
     3781 aatcagtaga tataattaga tagtcataaa ttaaattaaa atattttaat gagatatgac
     3841 ttttttagaa tgaaaaaccg tagtatattg atataataga aataagactt aaaaatctgt
     3901 tgatagtatt atttataagt acagtaggag gataaaatgt ttgaaaattt acctaagata
     3961 gatttacatt gccacttaga tggaagtgtt agagtagaaa ccatgcttga tatagctatt
     4021 aaagagaaaa tagatttacc ttcaaataat atggatgaaa taaaaaaatt agcaaaggtt
     4081 tcttttaatt gtacttcact agatgaatat ttagaaaaat ttgatttacc cttaaaagta
     4141 atgcaatcta aggaaaattt aaaaagaatt acttttgaac ttttggaaga tgcaagtaga
     4201 gaaaatgtaa aatatattga aattagattt gcaccattac ttcacaccca aaaaggtatg
     4261 agtgttaaaa atataattga gggtataatt gaaggaatta gagaagctga aagtatttat
     4321 gatataaaag gcaatttaat attaggttgt atgagaacaa tgacctcaaa agaggcatta
     4381 ttagtaattg aagaagggaa atcatttgta aataaaggtg ttgtagcagt agacttgtgt
     4441 ggtccagaga aagagggatt ttgtaaggaa tataaagatg tttttaaatt agcaagagaa
     4501 tatggatata aagttacaat acatgcagga gaagctgcaa gtggagaaaa tgtattggat
     4561 gctataaata ttttaaaggc ggataggatt ggtcatgggg taaaaattaa agaccatgaa
     4621 aaggcgtata atttagttaa agataaaaaa atattgcttg aattatgtcc aactagtaat
     4681 gtacaaacta agacagtaga ttcttatgag gtacatccat tttatacttt ttataaagat
     4741 aatttacatg taagtataaa tactgataat agaactgtat cagatataaa tttaagttca
     4801 gaactaaatg ttatatttga tacttttaaa ctaggattag aagactataa aataatttat
     4861 agaaatgcag tagaagctag ttttgcagat aaagaaacta aagagtattt gaatagttta
     4921 atttaataga taaaaatatt taaactttaa attaagctat taaattgaca aaaatacatt
     4981 gtattaggag aaaaaatgtg aaagattatg aagatgttgt ttatattaaa ctagtcaaaa
     5041 aagaacttga agaatggaag aaaaatatat taaagaaacc ttctatattt gacaaagcat
     5101 caaagggtat acagaataaa tttaatggag tattgccaga taaatatcat gaaataatta
     5161 cttcttctat aaaagtaatg acaaaaaccg tactgtttgg atataaatat gctacaaagc
     5221 caccacttag aaatatgtca ttagaagaaa gagaaaaatt agttttagaa aaagctaaaa
     5281 cttacaagac cacagcaatg attgaaggag caggaacagg ggcaggagga atcctcttag
     5341 gtttatctga ttttcctttg cttttaggta ttaaagtgaa atttttatat gatgtagctt
     5401 caatatatgg atatgatgtt aaagattata gagaaaggat ttatatatta aatatattac
     5461 agttggcttt ttctagtcaa cagcatataa acaaaacttt taaattgatg aataactggg
     5521 atgaatatgt aaatagtcta ccagaagaca tcaatgattt tgattggaga acatttcaac
     5581 aagagtatag ggattattta gatttagcta agtttttaca actaatgcct ggaataggag
     5641 catttgtagg attttatgtg aatggaaaat taattgataa attatgtgaa actgctgtgt
     5701 ctgcttatag attgaggatt cgagagctta agttataaga aaaattataa cataaaaaat
     5761 tagttataca atatatctat atgtatctaa ttttgcattg ataaaaacaa ttatggataa
     5821 aaggtaaagc atgatagaat ctaatgtaga ggtttgtatt ttaatggaaa aatttagttt
     5881 ttattgctta agatacaaat tgaattacga agagattgaa ttatgaagag aagtaattgg
     5941 gaggaatatt ttcatgacaa atttgccaaa acaatttgat agcttcaatg aagctagaca
     6001 aaaaggattt ataaatgcaa aggaacttaa ggaaagtggg aaaaagatgg ttggagtttt
     6061 ctgtacattt actccagttg aaattccaat ggcagctgga gcaactgtag taggtgtatg
     6121 cggcgttagt gaagagccaa tacctgatgc agaaagggtg cttccaagaa atctttgtcc
     6181 tttaattaaa tcaagctatg gacatgcaat aacagatacc tgtccttatt tctatttttc
     6241 tgatttgttg ataggagaaa ccacttgtga tggtaagaaa aaaatgtatg aagaacttgc
     6301 aaaagttaag ccaacatatg taatgcattt gccaaatact tcaaaaggag aatttgccta
     6361 taaactttgg aaagatgaaa tgataagatt aaaagaagag gttgagaaat ctttaggtgt
     6421 aactataact gaagaagata taagaactgc tataaaagat aaaaatgaag aaagagaact
     6481 tttaaaagag ttttatgcac ttggtaaatt acaaccatct gcacttacag gccttgagtt
     6541 acataatgtt ttatatcaag caggttttaa atttgaccgt gcagaactta aacattcttt
     6601 aagaaaagtt atagatgata tgaaagaaag atatgaaaaa ggtgaatgtc ctgttcaaaa
     6661 agataagcca agaatattga taacaggttc tcctattggt ggaataagcg aaaaaatagt
     6721 taaaacttta gaagatgcag gggcatcagt cgttgcatat gaactttgtg gagctattag
     6781 atcaaacgat ttattggttg atgaagaaat agaagatgta tatgatgctt taacacaaaa
     6841 atatataaat ataggttgtt cttgtatgat gaataatgac aatcgtattg aattattaga
     6901 tagaataata gatgaatata atgtagatgc tgtaatagat gtagttttac aagcatgtca
     6961 tacatttaat atagaaagtt atagaataag agaatttgtt acaaaagaaa aaaataagcc
     7021 atttatgtct cttgaaactg attattcaaa atcagatact gaacaattaa gaactagatt
     7081 tgaagcgttt gtagagatgt tgtaggaata aataaaagta ggaggaacta tgtttagtat
     7141 aggagttgac tcagggtcag tggcaacaaa gggtgtgcta tttgatggtg aaaaaattat
     7201 taagaaaata attataccaa ctggatggag ccctaaaagt acatctaaac aggtctatga
     7261 gttgctaagt agtgaaattg acaaaaaaga tataaaaaag gtggtaggta caggttatgg
     7321 aaggggagta atggattttg ctgataagaa agtaacagaa ataacttgtc atactagagg
     7381 tatttacttt ttaaacaata acataaggac tattttagat gtaggtggtc aagatagtaa
     7441 agttataaat ttagatagag atggaaatgt atttaacttt ataatgaatg ataagtgtgc
     7501 tgcaggaact ggtcgatttt tagagattac ttctaacctt ttaggaagcg atattgaaag
     7561 tatagataca ttagctaaag gatatgaacc agttaatata tctagtatgt gtacagtttt
     7621 tgctgagtct gaaattgtaa gtcttctagc tcaaaacatt tctactggag aggtagcagc
     7681 agggatactt aaatctattg caaataaatc cacctcaatg ttagcaagag gtgaggtaat
     7741 agatgaagtt gcatttactg gagggcttgc aaaaagtaaa gaactagtaa aaatgataga
     7801 agaaatactg ggtaaaaaaa tttttattgc agaagatact caaataatag gagctttagg
     7861 agctgctgta ataggattta gataaaaaca tataaaaaga aaatgagaag ctgaatttat
     7921 tataaataaa ttcagttttt tattttattt tatttattta taaaggaaaa ctttggtata
     7981 attgatttaa atgattgtat atttgtaaat acttgatatt tagtcatata taacattata
     8041 attttaaaaa ttagctcaaa ataaggggga gaaagcgtga aaaaatttac ttcaaaaaaa
     8101 gtaacaaggt ttgttgcgct gtttttaata tctattttag taatgtcaac aattcctgta
     8161 tcagctgaca atagcaatac attgaatcag ataaaaaaag cagaatacag tgagacgtat
     8221 aaacaatatc taaatgatgc taaaaatgga cgtacagaaa aatataatgg gattatacca
     8281 aatccatatc agttggaagg tactcaaatc caaagtaaag gaagaacagc tccaacatct
     8341 tatgacccta gaaaattagg tcttatgaca tcaataaaaa atcaagagga tttaggaatc
     8401 tgttgggatt ttgctgcaat ggcaacctta gaaagtttct taaagttaaa taattatgga
     8461 gactatgatt tatcagaaga acacttaaga tggtgggctt cagatggtga atatggatgg
     8521 tctgtaaatg atatgaatgg tgctttaaat tatgaagcta tgggatattt aacatcttgg
     8581 tcaggtccta aattagaaaa agatatccca tacaatggta gagtatctaa agcacaagga
     8641 gcaaaaaaac caactaatat gaatacagca cctactgttt ttaatgtgac agatgctgtg
     8701 tgtgtaagca atgacataaa ttctacaaaa aatgctatat tacagtatgg agcagtaaca
     8761 tctggttatt atgaagatat taaatatcaa agtgatgacc aaaattcata ttattgtcca
     8821 acaaaaagtt ttaatacaaa tcatgctatt tcaattgtag gttgggatga taactattct
     8881 aaagataatt ttaatgcaaa tattagacca tcaaaaaatg gtgcttggtt aataaaaaat
     8941 agctggggag attataattc tgagggtgga tatttctgga tatcttatga agacaagaca
     9001 cttatgtctg atatagataa tttttcaatc aaaggtggta aaaaaccaaa tgatgatgag
     9061 aagatgtatc aacatgacta tgcaagtatt gtacctttaa tttcaaaaaa gataacagct
     9121 gcaaatgtat ttgatttcaa tagaggtgat gaaactctaa aatctgtaat gttcttaact
     9181 gaaagtatag gagctaagta tgaagtatat tatgctccag tagtaaatgg tatacctcaa
     9241 gaaaacaata tgaaaaaatt aaaagaaggt actgcccaat attcaggata cataacagtt
     9301 ccaattgatt cttttgatat accagaagga aagggtgcaa ttgtagtcag catagagggg
     9361 aaaaatggag agtcaactat aggttctgag tcaaatgtac caggatatga tacatttaaa
     9421 gcaaaagcta atttaggtga aagttatatc atagataata ctggaaaatt ctttgatata
     9481 aatagagata gtaacttcta tccgtgtaat tttactataa aagctgttac agaaaagtct
     9541 tctggtgaga gtatcccaaa tgagtcttta attggtagtg atagatatga aactgctatc
     9601 aaagttagtc aaaatggttt caattcttca gaaaatgtag ttttagtaaa tggcagttca
     9661 atagtagatg cattggcagc aactcctttt acatctgcta taaattcacc aatattattg
     9721 acacaaaaag aagctttaaa ttcaaaaact aaagctgaga tacagagatt aggtgctaag
     9781 aaagtatacc taataggtgg agaaaattct ataagtaaag aaattgaaca acaattaaaa
     9841 agcttaaata tatcgataga gagaatatca ggaagtgata gatacaagac aagtttatta
     9901 ttagctcaaa agttaaatgg tataaaaaat gtatctcaaa tagcagttgt aaatggagtg
     9961 aaaggattag ctgatgcaat tagtgtaggc gctgcagcag cagaaaataa cataccaatc
    10021 atacttgcaa atgaaaagag cgaattgcaa ggagctgatg aatttctaaa ttcattaaat
    10081 atagagaaat cttatattat tggaggaaca gcatccctat caaacaatct agaaagtaag
    10141 ttaaaaaatc caactagatt atctggtagt agtagagacg aaactaattc aaagataata
    10201 gataattttt ataaaaaaga tactttaaaa aatgcatttg tagttaaaaa tggaatcaag
    10261 aatcaaaatg atttaataga tggattggca gttggtcctt taggagcaaa aactgggtca
    10321 ccagtaattt tagttggtga taaattagct gatagccaaa aagaagttct taaaaataag
    10381 actttagaaa aagtaactca agttggtgga ggagcaaata aaaatgcatt taaagagtta
    10441 gtaaaattaa aaagtgctaa atagagtact taagtattaa catatattag tgtgtattag
    10501 taacttatct taataaaagc tgaaaataat tacttcaaat tattgataaa taagataatc
    10561 aatgattgta aagttttgtt ttcagcttta aatttatgta gtataagttt aaaaacataa
    10621 aaaagggtta ctttatatag aaatatagaa aatcttacat tttatatata aataacccat
    10681 atttaattaa ctttttatag tgttctaata gatattattg aacttaatgg taaatataat
    10741 gtgtaagttt catttaaagt tggattagat tcatctatat atcttgagca aagtgtaatt
    10801 gataagctat ctgcttctat aatttcacca tatagaggac aactgctaaa gtaagaatgt
    10861 acttcaacat atttatttaa gttttcttga acataatcgt aaaaacggtt ttttgtagta
    10921 gtagccataa aatcaactcc tttatttttt attaaagttg aaacttttaa aaagataaat
    10981 atgattactt ctattagaaa ctaagttaaa aaataagcaa atagatatga aaattagaat
    11041 aatatattat ttatatctta attataacac tagataataa aaaatgctat ataaaattca
    11101 gaattttcga aagtttttaa gcttattttt aaatatacta ataatactat tgacattaga
    11161 tagaaaaagt attatattga ctatataaat tattttagtc aaactggagg gaaaagatgc
    11221 ctaaatcata ttctgataaa gaaagagagt atataataaa aagacttaaa gaagaagcaa
    11281 gattgtgtat ggagcaatat ggtattagaa aaactactgt tgatgagctt gtaaaacggg
    11341 taaaaatacc aaaaggaact ttttatctat tttttcaatc aaaagaattg ctcttttttg
    11401 aggtattaag agatatacat gactctatac aaaaagaaat cttatatgag ataaatagag
    11461 tagatgaaag tattacatgt gaacaattga cagatatatt tatgaaattc tataggatgg
    11521 ttgatagtac ttcaatttta aatctcatga taaatggaga atttgagata ttggtgagaa
    11581 agttaccaga ttctataatt gaagaacact ttagacatga tgactttgaa attggagaga
    11641 ttatatcttg cataccaaat gcaaaaaata aagacataga aagctttagt ggagcattta
    11701 gagcagtttt tcttaccatg ctatataagc gcgaggttgg aagtaattgt tttgaagatg
    11761 ctttaagatt aatgataaac ggattagtta tacaacttat ggaatagaat ttaaattttg
    11821 tttgataata acttttatta aaaattatta acaaacaaaa aaattatatt ttattgacca
    11881 tatagtcaaa atagtcaaaa atttgaaagg gtgatattga atgattaaag ttgatgattt
    11941 atcatttagt tatacagaga gagatttttt acaaaatatt aactttggag taggaaaagg
    12001 ggaaatacta ggatttttag gaccatcagg tgcaggaaaa tctacattac aaaagattct
    12061 aattggaatg ataactaatt atggaggaag tgtcattgta aatggagtag agagtaaaag
    12121 acactcaaat aaattttatg aaaatatagg agttgatttt gaatttccta gtttatatga
    12181 aaagttgact gcaatagaaa atttaaaata ttttgggtct ctatattcta aaaaactact
    12241 atctattgat gagttattaa aatcagtagg actagaaaat gaagcaaata aaagggtatc
    12301 tgagtattca aaaggtatga aatctcgatt aaattttata aaagctttat tacataatcc
    12361 agatatttta tttttagatg aaccaacaag tggtcttgac ccatcaaata gcaaggttat
    12421 gaaagatata attttatcag aaaaatctaa aggtaaaact attattttaa caactcataa
    12481 catgttagat gcaacagagc tttgtgacag agtagcgttc attgtaaatg gtaaaatatc
    12541 tgctttagat acaccacata atcttattat gtcaaaagga gctattaaag ttagatatac
    12601 ttactttgat aatggagaaa aaacttctga atgttttctg aacaatactg ctaatgataa
    12661 aaatttgaat atgcttattg agaaaaataa acttttatca atacatagta gtgaaccaac
    12721 attaaatgat atttttattg agataacagg gaggaattta caatgaggct aaaaaatctt
    12781 attttaggag atataaagtt tcaatttaag tatggttttt attttttata tcttttttta
    12841 agtattatat atatttgtat tatcaatgta tttccaactt ttatgagaga aaaaatagct
    12901 ataattatga tttattctga cccagcagca atgggattgt tttttatggg agctattgta
    12961 cttcttgaga aaagtcaaag ggttttaaat tccttagctg tatctccagt aaaagtatct
    13021 gagtatattt tatcaaaggt aatttcatta ggagtaatat ctagtattgt agctatgttt
    13081 attgctatta cattaaattt agataatatt ataatatcaa ctataggtac ctttttttct
    13141 tctattatat tttctttgct tggattgata cttgcaagta aagcatctag tttgaatcag
    13201 tttattgttt taagtattcc tattgagatt atatgcttta ttccaccaat actcaatgta
    13261 cttcttgaca caaaatcata tgcaaatttg taccctttta atatatgtat ttctcttatt
    13321 agtggagata aaaattttat aatgattaat attttaattt taatatcaat tataataata
    13381 atttatttta taacttatta ttttatttgt agcagttgga aaaaggtagg tggagtaaaa
    13441 ctatgataaa gattataaat gcgtttaaac aagagtttga acaaattaag agagatgcta
    13501 tgctttttat agtatgtgtt tctccaatat tatgtggagt atttataaaa tttggaatac
    13561 cattaataca gaatatatca cttaacaagt tttattatca attaaattta gagccgtatt
    13621 tcttgatgtt tgatttactt ttagctttta ttacaccttt tatgttcttt tttgcttcta
    13681 caatggtcat attgggagaa attgatgaca gcatttcaag atatttaata gtaactccgc
    13741 ttggtaaaac tggttatctt atttcacgat ttggaatacc aggtatctta gcatttatta
    13801 ttactatgat tctacttatc tttttttctc taactaaaat ttctttcttg ttaaacttat
    13861 caatttcttt attagtttta ttacaaggaa ttattatatc aatattagtg atttctttat
    13921 catcaaataa actagaagga atggtaatta cgaaattctc aggtgttttt atgatgggta
    13981 ttttagcacc attttttatt ttaaataaag cacaatatat tctgttcttc ttaccaactt
    14041 tttggatatc gaaggcattt aaggaagata attatgtcta tatgtttatt tcaattgtta
    14101 tatcattaat atggatttta ttattattta aaaagtttag taaaaaaata gttaaataag
    14161 aaaatttaat atataaatgt aaggttgact tgacagtaat gttgaaaaat gttacattta
    14221 agatgtaaag ttaaccttac atgaatggag caaatatgaa aaataaaata aagatattaa
    14281 gagaaaaatt aggattgaca caagaacaac tagggagatt agttggaaca tctagacagg
    14341 ctataaatgc tatagaaact ggtaaaaatg aaccttcaat atggttggca tatgatattt
    14401 caagaatttt taatgaacca attgaaagtg tatttttatt tgaagaaagt gaaagaaaat
    14461 caagagcaca gataagtagg ggagaatatt atggctataa gaaagataag aacatttgat
    14521 gatgaaattt taagaaaaaa gagtaagtat gtagaaaacg tagataataa gataagagaa
    14581 atattaaatg acatggcaga gaccatgtac aatactccaa atggaggagg attagctgca
    14641 tgccaagtag gagtattaaa acgattagtt gttatagatt taggagaagg acttattaag
    14701 cttgttaatc ctgaaattat taaacaagaa gaggaacaaa ttgttgttga aggatgttta
    14761 agttttcctg aagtatgggg taaattaaaa agaccaaaaa aagttacagt gcaagctcta
    14821 aacgaatacg gtgagaaaat agaaattaaa ggttcaggtt ttatggcaaa atgtctttgt
    14881 catgagatag accatttaaa tggtatagtt tttacagata aaattattga acatgtaaaa
    14941 ttgtaagata gattggttat tgaatttata aaatatttta tagtaaggag ttaaattatg
    15001 agattatggc ataaggattt aattgatgta ttacctaaaa atcaattagt aagtcaatgg
    15061 agagagctac ttgctataaa aggttctata gataaaaaag gtactccaaa ccatctatta
    15121 gtgaataaag tattgaatta tagtatagat gaatttaaat tttatacaaa aattgtacat
    15181 gatgaaatgt taaaaagaaa ttataaacct aatgaactta aatatacaag tatattaaaa
    15241 tggaaaaata gaaattttgc aaatgatata tctaatgaac atagtttaaa tttagaaaat
    15301 ttatatgatg attggcataa taaaatgtat ttaaagcaat gcttatataa tttagaagaa
    15361 aaagcaacat gtggaggaat accaataaat gaatggaata tattactttg taaatatagt
    15421 aaagattatg aattgtggtc tggaaatatc atattttaat tactatagat taacattata
    15481 gtattattgt atataaagag gtgattattt gtataataaa ataaaatata ttccaataaa
    15541 ttgtgaaata gcttgtaata aattaaaaag gagtatgcca tataaatggg atttaaatat
    15601 ttatcgtgga tgtgagcatg gatgtaaata ttgttatgct atttactctc ataagtacat
    15661 caactccaat aattattttg aagagattta tgtaaaaaca aatatagtag aaaaacttga
    15721 gagacaactt aaaagcagta aatggaagaa agaagtaatc aatataggag gagttacaga
    15781 tagttatcaa ccaatagaag ctgactataa aattatgcca gaaatattaa acctgctgat
    15841 aaaatacaag acaccagcta ttatttctac aaaatcagat ttaattttaa gagattacga
    15901 tttaattgat aagctatcta ggattacata tatcaatgta gcatctacaa ttaccacagt
    15961 aaatgaagat acacaaaggc ttattgagcc aaatggtgtg gattcaatga gacgatttaa
    16021 aatgttaaaa gaatttagaa aaactaacgc atcagttggt ctacatatta tgccaattat
    16081 accatatatt acagatgatt ttgataatat aaattcgcta tttagacatg caaaagaatg
    16141 taatgtacat tatgttttac ctggggcttt atacttaagg ggtataacta gaggtgtttt
    16201 ttttgacttt gttaaaaaag agtttcctga attatttgat aaattatcaa tactatatag
    16261 tgcaggttca cttaataggg agtataaaaa tcaattgtat aaaatggtta atgaacttag
    16321 atataagtat tctctatcaa gcagttactc taaaataatg aaggaaaagt taaaaaactc
    16381 aggagatgta cagatatctt tttttgatta gttttatata aaaaaatttt ttttactatg
    16441 atgattaaaa tttattaaaa ttaaaattta ttgacaaata taaaatttaa atatataatt
    16501 aattcataaa ataaaaatat gaaaggatga tgttgttcgg tgatatttga tatcataaaa
    16561 tcgttgatat aagtattgct ggaaaagagt gaataaaatc aaatttaata agcaaacaaa
    16621 gtaaagctat agttttattg ttgtgcttaa aaatatttgg ttctatttta ctcttattta
    16681 aactcatctt tttgaagtta aaccacagag taaactctgt ggttttttta ttttaaaaat
    16741 aaattttaag gagtggtatg ttttgttaaa caaattatgt atgtatttag aaagaccaga
    16801 gctttataaa caaagtgaaa ttaatttttg ggatgatgag tatatttcaa agcaattatt
    16861 aaaagcacac ttagacacta attttgaagg agcaagtaga aattttaact ttatcgagga
    16921 ttctgtaaat tggatagtta cagttgcaaa tccagctaat tatcctaaat tacttgattt
    16981 aggttgtggt ccaggtttat atgcagagaa atttgcacaa aaaggttata atgtaactgg
    17041 aattgatttc tcaaaacgtt ctattaacta tgcacaaaat agaaataagg agactaattt
    17101 aaacataaat tacttgtttc aaagttattt aaatatgaac tataatgagg aatttgattt
    17161 ggctacttta atttattgtg attatggagc tttgtcaact gaaaatagaa ggttattaat
    17221 ggaaaaaatt ta
//
LOCUS       DAEMIK010000089        17201 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEMIK010000089 DAEMIK010000000
VERSION     DAEMIK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.089"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(114..638)
                     /locus_tag="KPK87_002435"
     CDS             complement(114..638)
                     /locus_tag="KPK87_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2538579.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(703..1455)
                     /locus_tag="KPK87_002436"
     CDS             complement(703..1455)
                     /locus_tag="KPK87_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2538580.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1486..2784)
                     /locus_tag="KPK87_002437"
     CDS             complement(1486..2784)
                     /locus_tag="KPK87_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG2538581.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2801..5065)
                     /locus_tag="KPK87_002438"
     CDS             complement(2801..5065)
                     /locus_tag="KPK87_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG2538582.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5320..6138)
                     /locus_tag="KPK87_002439"
     CDS             complement(5320..6138)
                     /locus_tag="KPK87_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2538583.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6131..6829)
                     /gene="cbiQ"
                     /locus_tag="KPK87_002440"
     CDS             complement(6131..6829)
                     /gene="cbiQ"
                     /locus_tag="KPK87_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG2538584.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6832..7116)
                     /locus_tag="KPK87_002441"
     CDS             complement(6832..7116)
                     /locus_tag="KPK87_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2538585.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7106..7858)
                     /locus_tag="KPK87_002442"
     CDS             complement(7106..7858)
                     /locus_tag="KPK87_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG2538586.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8040..8232)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8364..10136)
                     /locus_tag="KPK87_002443"
     CDS             complement(8364..10136)
                     /locus_tag="KPK87_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG2538587.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYFSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKDEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10150..11079)
                     /locus_tag="KPK87_002444"
     CDS             complement(10150..11079)
                     /locus_tag="KPK87_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2538588.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11308..11496
                     /locus_tag="KPK87_002445"
     CDS             11308..11496
                     /locus_tag="KPK87_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538589.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11489..12190
                     /locus_tag="KPK87_002446"
     CDS             11489..12190
                     /locus_tag="KPK87_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2538590.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12192..13115
                     /locus_tag="KPK87_002447"
     CDS             12192..13115
                     /locus_tag="KPK87_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2538591.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13272..14195
                     /locus_tag="KPK87_002448"
     CDS             13272..14195
                     /locus_tag="KPK87_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538592.1"
                     /translation="MNDFVIETKQLTKTYGDQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14188..14925
                     /locus_tag="KPK87_002449"
     CDS             14188..14925
                     /locus_tag="KPK87_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538593.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14936..15709
                     /locus_tag="KPK87_002450"
     CDS             14936..15709
                     /locus_tag="KPK87_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538594.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYVDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPITAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16250..16858)
                     /locus_tag="KPK87_002451"
     CDS             complement(16250..16858)
                     /locus_tag="KPK87_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG2538595.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17036..17152)
                     /gene="rrf"
                     /locus_tag="KPK87_002452"
     rRNA            complement(17036..17152)
                     /gene="rrf"
                     /locus_tag="KPK87_002452"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5531 a         2992 c         2178 g         6500 t
ORIGIN      
        1 aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct catttttaag
       61 taatatatat ttttaatttt tatttataac taaatctagt ttttatataa attttagttt
      121 ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt cataacttgt
      181 ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg ctatttcatc aactgtatcc
      241 ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg tgagttttta
      301 tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc cattgatatg
      361 actcctcttt catgttttaa attgttagta aatccattac cactaaattc accttttatt
      421 gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat cataaatcct
      481 ggtattactc tatgaaatat tattccattg taatatcctt catttactaa tgatacaaag
      541 tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc ttttccattt
      601 tccatttcta tagttactat aggattttta ttttccataa tttattacct tccttttcat
      661 tagttaaatt acataattac atttattata ttatactcta aattaaagtc tatcaagtat
      721 attttttcct atttcatatc cttcttgata aatagtttta caaaactcat catcaaagtt
      781 aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt tatcttcatc
      841 atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata ttaaaataat
      901 tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta aacctccatc
      961 taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt ctttaaatcc
     1021 aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga taatggcttc
     1081 attacgaggc atttttgata cgtcaacaat cttttctgat actactttat tatcaacttc
     1141 aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt cccttaggtt
     1201 gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc cattctggat
     1261 atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt acaaataata
     1321 tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta ttcctccatc
     1381 atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac cttctaaaca
     1441 taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata tttcaaaaaa
     1501 cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt ctcttataga
     1561 atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat gagtagaaga
     1621 tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt cttgatactc
     1681 aacattagct tctctacata tatttttata aatagctata gaatatccat cactagtata
     1741 tgcctgacta gcatttatct ttataaccgg accttttccc attattggtt tattagttgg
     1801 gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg atatcataaa
     1861 agatgaataa atagactcaa agaatccctc tctatcctta cccattgata tgcaaattct
     1921 ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc taccaacttc
     1981 ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat taacaagagc
     2041 attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata taaattcatc
     2101 attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa aatctataat
     2161 atcttccata tttacatcta agttagatgc tatttgcttt ataagaaaat tatctttttc
     2221 taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat tatatgaatg
     2281 accatcattt atacttctat ttaaatgtat tgctagattt ggtattatac aaattggctt
     2341 tttaaagtct ataagccttt catttggttt taatatacta tcacctttta aaactactct
     2401 accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc catagcattc
     2461 tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt taatactaaa
     2521 tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta tatcttctga
     2581 atttactaca aaagctacta tagctgataa attcttagta acataatact ttccaccaac
     2641 tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat ctaagatttc
     2701 cttagtactc tcgactgcat gaaaagacga cggactatca tatatgaagt ctattaagtt
     2761 tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata tttagtgttg
     2821 tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta acatctgtac
     2881 caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta gaaccaagtt
     2941 ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta actttatctg
     3001 caactttaga tttgcttata gtactcatat actttttagt atatttatct aacatattat
     3061 aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct atacattctt
     3121 ctggcagttc ttccataaat cttgaagcta ttgcaacatt tgttttacca tataatgttc
     3181 tcttctctgt caatgacata tatagctctt ctttagctct agttatacca acataacaaa
     3241 gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt gatataggaa
     3301 ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct ttggatgtat
     3361 gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca gatgtaagtg
     3421 ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca ttactacttt
     3481 caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct tgagcttctt
     3541 cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc tcgtccatat
     3601 atcctgtagt atccaagact ttttctataa gtttgctaac aggataaact tcttttatag
     3661 ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc ttagtagata
     3721 tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt tttaagttag
     3781 ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga acatttataa
     3841 tcctttttat agatatatca tcttgtggat tttgaataac tcttaagtac gccaacaaat
     3901 cctttatttc ttttctctca tagaattttg taccaccata tatattgtat ggaatttgac
     3961 ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa agtactgcaa
     4021 aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca gcaatgaaat
     4081 cagcctcttc tatttcgcta ccagttaatt gtatctttat aagttctcct tctttttttt
     4141 cactccaaag cttttttctt tttctttcta tattattaga tatgacttta ttagctgcat
     4201 ctaatatcac ttgtgtagac ctataatttt gttctaactt tacaacatgt acattgtcat
     4261 aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca taaatacttt
     4321 ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat tttatcaatt
     4381 catattgagc tttacttgta tcttggtact catctaccat tatgtatcta aatctacttc
     4441 tgtaatatgc taacacttca tcattagcct tgaaaagttc tacagtttta agtattaaat
     4501 catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca taaatatctg
     4561 caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt ggagtataaa
     4621 gtttgtcttt tgctcctgaa attgcactta taactgcttt tggttcaaac actttatcac
     4681 ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct gaatcataaa
     4741 ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata cgaacacagc
     4801 aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt tgttcaactc
     4861 tctctctcat ttcatttgca gctttgtttg taaatgttat agccaatata tttggtgctt
     4921 gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta gttttacctg
     4981 aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct tctctttgag
     5041 caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata aatatatttt
     5101 aatctaaaat atgaattact tcaaattata attatgtatt atattaatag attatttcca
     5161 atatattaca attaaataac atttttccat ataatttatt tcattgttta ataaaaaaaa
     5221 taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta agggttatta
     5281 tatataaatc tattttttta aaataggttt agggcttaat tatttatttg tattaagttc
     5341 tctttccttc caatacttat acaaatcttc ttctttctta aataagggta aattcatatt
     5401 tctatgtact tttactaacc atggggattc tagaccagct tgtataatat tattttcatt
     5461 tataaacaca ttttctgcct ttccttcatc aatcaaaatc cccttatcca atacatatat
     5521 gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta tttttatatt
     5581 atttttattt aatcccttta ttatatctac aatagaccta gtacttactg ggtctaaacc
     5641 agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg atgctattgc
     5701 cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa aatctattcc
     5761 attaacagct actagagctt tgtttattct ctctttaatt gttttttcat ccataccaat
     5821 gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt gtttttctgg
     5881 gtcttggaac acaataccta cattttttct aaggttgtat aaacttctct tatcatattt
     5941 taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac ccattaaatt
     6001 cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa catttccctt
     6061 tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt gatacgttaa
     6121 attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca tacaatttta
     6181 tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac agcatacttc
     6241 caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat cttagctctt
     6301 gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta tatattaaca
     6361 ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat attaattgat
     6421 taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc gctctaaata
     6481 acacatgaat agacatgtca atagacgcct ttgatattcc tacattaaaa ctaaaaaatt
     6541 caaaactata taataaatct gctttattaa aagatatatt tacaagagtt attcctatac
     6601 taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt aaatctattc
     6661 cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa ataaatatat
     6721 tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt ttgtttggat
     6781 ttactttact caatctattt gtatatgcat atttatcaat tataagcatg attaacttct
     6841 ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag ctgcttgtgc
     6901 agagaataat aaactttcta tttctccact tggtggctca tatggactgc taaaccatgg
     6961 tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg ctcctccata
     7021 ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta ataaaagtaa
     7081 taatatgtta gtttttgttt ttgttttagc actcattagc tttaactcct ttatcattat
     7141 tttctagtat gcttttttct ccgttttctg taattaaatt atatacaatt acagtaagta
     7201 taccttctgc tattgctatt ggtatttgag tcataaagaa aattcctaaa aatttaattg
     7261 ctgaagccat taccccacct gatgggtctg gaaaagccag tgctaattgt attgaagtta
     7321 ttgtatatgt ggctaagtct cctattgctg ctgcaagaaa tactggaata gcattatttc
     7381 catctttttt ctttaaagcc ttaaatatta ggaatgatac gattggccct attatcgcca
     7441 ttgaaaaagc attagctcct aaagtagtta ttcctccatg tgctaataac aatgcttgga
     7501 atattaaaac tatagttcca agaacaaaca ttacgcttgg tccaaacatt attgctccaa
     7561 gacctacacc agtaggatgt gaacaacttc ctgttactga aggtattttt agtgctgata
     7621 atacaaacac aaatgctcca cataaagcta ataatacttt ctttttaggg tcttgcttta
     7681 ctattttacc tattgatttt aatccgaata agaaaaatgg tataaatata actccccatg
     7741 ctatagacca ttttactggt aagtaacctt ccattatatg cattgcattt gatgtaactg
     7801 gagttaaaat gattaacata agtgcagcta taacccctaa tttaatattc tgtttcatct
     7861 tagtatccct ctctcccttt ctaataatta aaacataata atacatgtct gatttattta
     7921 ataaaataaa aaagcctaac tcgatggtta ggcttctaat tttccacaat ctaaatacaa
     7981 acacataaaa caaacatatc tgtataatta ttataagaaa ctttctccat ccccgaagaa
     8041 attactttaa tattttaggc aggtctcctg acttagcttc atcctactcc ttttccttcc
     8101 catgagatat aaaaaatatc atcatagtgg ctaacaaaag tttcgtctgc atcacagtag
     8161 cgggggctgt aaaggaattt aacctttttc cctattaatc ttgcttaaag aacctataat
     8221 attatatatt taatttttaa attattctta aattattttc tatataaatc attgtatacc
     8281 ttttctgtga aaatttctac attttttata aattttattc atttttatat ttttatgtat
     8341 ataaattctt ttttgtaagt ttattaattt ctaattatct tctttaaata cattgaaact
     8401 acatcagaaa atctagttat atctcttgaa tatataaaat catttccata tattaatctt
     8461 tcagcttcaa gttctgattc ttttcctgta aatactccta atactaatat attttgcttt
     8521 ctagcttttc tcacttcatt ggctgtatct ctcaaaccaa ctatacccct ataagccttt
     8581 tctcctctta atgttctctc actacttttt cctatcttta catcatttgg tcttccatcg
     8641 ctgagaacta tcaggatttt attttcttca tctctattta ttagaccact acatatagat
     8701 tttattgcca aaccatctct attattacca gcacaaaaat attcaaatat attttcacta
     8761 ttttttatac tatcatcata atctttaaat ctttttaata ttgtatagtc taaaaaacta
     8821 gaaaatccca tcactctatt tggtatgcca acttctgtta gagcatttgc aattatgtaa
     8881 gcttgaattg ccacatttcc ctgatttcta ctttgagagc cacttgcatc caataatata
     8941 tcaataacgt atttcccttt ttcattttct atatctttat aaaatatttt attattatta
     9001 cttctaccaa ctctccaagc tctatttgct aatatagtac cacaatcaga atatactcta
     9061 cttttagcat tttcttctat taaaattctt gacagactct cttttaatct ctttatttgc
     9121 tgtctataca ttctagcatg ttctctaaat tttcctaaat tattttcttt ttgtcttgtt
     9181 acatatttta gttgaaatac attattacac tcacttctaa gaactccatc tgtaaagtgt
     9241 attctacaac cttcatgaac atttctacaa ttttgtgtct caattttttt aatttcatct
     9301 ttactcaaat aagtttttcc ataataatgt tctatttttg aatatatctt atttgcagtg
     9361 tcttcatcaa catatataac tcggtctgct gaattgctac atttagaatc atcaaaatcc
     9421 cctacacctt ctacaagcat ggatgaagat attgaatcta tatcactttc aataatattt
     9481 tcttcttcat cactataaag ttcttcatac atgaaatcag caaatgtatc aaaatttact
     9541 tctacacttt ctacattatt ttcatcaata agactatttt cttctccatt taatatgtat
     9601 tcaaaataag ttaaatatat aacatctaat gcttttaaaa tctccataat atcttgattg
     9661 gtatctatat ttcttatatc attaactatt cttctagtca tactatctaa tgctgggtgc
     9721 ttgtccatac tataaagtac aaatgtgtat ttaattttat ccataatatt atcattgtgt
     9781 attttggtaa aacgtgaaac aatatcttta taagcatttc ttcttatgtc ttctatccct
     9841 ggcctatctt taatgacctt ttcttcaact attgtattta atatgacttg aattagaccc
     9901 agtataatat ctttgttgta tccttttctt attctatttg taagatattt tttaactata
     9961 tcccaatcta catactttct tctagcacct gcaattattg cgaaatatag acctatttct
    10021 tttgaaaagt aatctttttc tgatatatct atactttcat tgtaatcacc agctattgtc
    10081 catgataggt tattagctct attttcaaat tcataatcat catatatcca tttcataatc
    10141 tcacctatat taaatatgac ttattggaaa cacatctata ctctcccatt tattaggtat
    10201 tctagtttta attacatctc caaccatttc tttctcatat acatcaaaag tctttcctgt
    10261 aagtcccata ttaattgcaa gagttggttt taatcctctt cttatagtct taagagatgc
    10321 cataagacca cgtaaatcaa tagctttact agatatctct ccattttgag acttcaattg
    10381 taaatctaaa aatattcctg caaagtgtat taatttttct tcatcagcat ctgaaaattc
    10441 attttttaaa atcatcatta acttatcttc ttctatagga ggaatatcta ttaccataaa
    10501 tctagatacc agtgcttcat taagttcttt tgttcctgca tattcataat tcatagtccc
    10561 aataaatctt gttgctggat gtagattgac tctctcatac ccaggtacat caattattct
    10621 tctatagtct aaagctgaat gtaccacaac tatagcatca ttttttgcca tattaatttc
    10681 atcaaatacc ccaaaacctc cattaatagc acactcataa actgagcctc ttctaagttt
    10741 aacttcattg tctataaatg tatctgtacc tataagtgtt gaactgtctg tatttatatg
    10801 aaatgatgta ttccactgag gtctattaaa tatttcccct agattatcag caagtaagtt
    10861 ttttcctgtt gcctttggtc ccgataataa tatattttct tcttctaaaa tagctgctat
    10921 acacatagac aagatatctt taccataaaa ataagtttta gactttgtta ctctgtattc
    10981 taaatctttt tctaatccat aataatttct aaaatacagt acatcttcaa taagtttttc
    11041 atctacccct tgcaccctta gattatctat tatatccata tttttacccc ttttatataa
    11101 atagtatttg tttcatttct tatcaccatt ttagcataaa aggatataag ctaaaattga
    11161 ttttttaatc tcccccattt taagtaaaac catgcaccat tttatcacta caaaattaag
    11221 agtactactt actgatattt cttgatttta ttcgtctata cgtatataat atatgctaat
    11281 aaattatgag gaaaggatga ttgaaatatg gaatatatgt cttgcccaga agtagcaaaa
    11341 aaatggggta tttctgaaag aagagtacaa aaactatgtg aagaaagtcg cataccagga
    11401 gtttcaaaac tcgggtatat gtggctgata ccaaagaacg cagaaaagcc tgttgatggt
    11461 agaacaaaaa gaagaaagga agtacaatat gaatagcgtt ttgattatag atgatgacaa
    11521 agaactttgt gctcttatga aaaaatgtgt tgagcaagaa aatttatctg ctattactgc
    11581 acatggtggt gcagaaggtc tacatttagt cgataaaaat agacacacac tctctcttgt
    11641 aattttggac ataatgatgc cagatataga cggttttcaa gttctgaaaa aaattagaga
    11701 aacaagtaat attccagtaa ttatgcttac tgctaaaaat gacgaggatg ataaagtttc
    11761 cggactacgt ttaggtgctg acgattacct aacaaaaccg ttcagcataa acgaacttat
    11821 ggctcgtgtc aattctctta ttcgtcggta tactatgtta aaccctgctt ttataacaga
    11881 cacagattgc ataataataa atggcatgat tattgacaag acaaaccgaa ttgtttctgt
    11941 caataatatt cctgttcaac ttacaagcaa agaatttgat ttacttgctt ttctggcttc
    12001 taataaggga cgggtgttta ccaaaaaaca gatttataca caagtatggg aagaagaata
    12061 tgcttttgac gatagtaaca tcatgtcttt catcagtaaa ttgagaaaaa agattgaacc
    12121 agaccccaac catccatttt atattttgac agttcgtggt gtcggttatc gttttaataa
    12181 ggaggcttaa catgaataca agtgtctatc tgcttttgat attggctctt agcctgatta
    12241 ttatagcgta ccttgtttct gtcctgctac gtgtccgaat acaacttaca cttataaaag
    12301 atgctttaga ggatataaaa aatggaaacc ttaatcgacg tattttaaca gatgaaaacg
    12361 acattacaaa acaaatttgc tatgatatta acgaaatagc aatcaatagc caatttcaac
    12421 ttattcagca aaaacaatca gaacaagcct acaaacagct aatgacaagt ctctcccatg
    12481 atgtaaaaac tccacttgct tctttggtcg gttatttgga agcagtagaa agtaaaattg
    12541 ttgtaggaga ggaaaaagac gagtatatcc atgttgcgtc taataaggct cattacttaa
    12601 agaattttgt agaaaatcta tttgaatggg taaaattgga ttctaaggaa caagtttttc
    12661 attttgatat ttttgacttg aatgaattat cccgtaatat tatatctgat tggatacctg
    12721 ttttagaaag cagtcatttt gaatatgaat ttgatatacc agaaatagag tacttcttac
    12781 gtatagacgc aaatgcatat acccgtatca ttaataacct attacaaaat attattaccc
    12841 atagtagtgg caacaaaatg acattgagaa tttttgaaaa caaagagcaa gctcaaatta
    12901 taataacaga taatgggaaa ggtatatctt cagacaatct cccacatatt tttgaaaggt
    12961 tgtaccaatg cgaccattca cgagcttcaa agggaaatgg tttaggcttg gcgattgcaa
    13021 aggaacttat taacgctcat aagggaaata ttaccgccaa cagcactccc ggtatgggaa
    13081 cagaatttac aatattgctt cctaaagccc tgtaaaagta cgaggctttc ttactctaaa
    13141 acaagataaa agcaagatta tggcaaggtt ttggcaaggt tcatgtttta taattctaat
    13201 tgtagttgat tttgacgttg tatcaactat cttttgtaga aacaaattta ttaaaagtga
    13261 ggaaattata tatgaatgat tttgtgattg aaacaaagca acttacaaaa acttatgggg
    13321 atcaaacagt agttgacaaa gtcaatctcc atgtaaaaaa aggccgaata tatggtctgt
    13381 taggacgcaa cggagctggg aaaaccacaa ttatgaaaat gattttagga ttgacttcaa
    13441 tcacgtatgg cgaggtagat gtatttggag agaatattaa gggacgtgaa aaacaagtat
    13501 atccccgtat cggtgcaatt atcgaaacgc caggatttta tcctaatctg acaggaacgg
    13561 agaacttgga gatttttgca aaactgcgtg gtacagccgc ccctaattct gtaaaaagag
    13621 cattggaaat tgtaggctta ccttataaaa ataaaaagct gtttagtaaa tattcacttg
    13681 gcatgaaaca gcgacttggt attgccaacg ctatcctgca tgacccagaa ctgttagttt
    13741 tagatgagcc aaccaatggt cttgacccta ttggtattgc ggagatgaga aagtttatta
    13801 gggacttgag cgttgagcat ggaaagacaa ttcttatttc cagtcatatt ctttcagaaa
    13861 tctcattgtt ggctgatgat attggaatta tcgaccacgg tgttcttttg gaagaaagta
    13921 gtatgagtga actagaaaag aaaaacagta aatatatcct gctacaagtt tcagatattc
    13981 cgaagacatc acttatttta gagcgtcaat ttaacacgaa ggattattcc gttcaagatg
    14041 actacacgtt acacctttat aacactaatc tggatatggg tgaaatcaat aaagcacttg
    14101 taatgcagaa tgtaattgtt attagttctc aactttgtaa tgatacttta gaggactact
    14161 ttaaaaaaat cacaggagga gagggcattg cttaaactta ttcaaacaga atttcttaaa
    14221 ctgcgtcgta ggaaacttat ttggctgatg ttgttgtctg ctctcattat gccgtttttc
    14281 gcactactat acttcaatta ttttggtaaa acaggtgttg agccaacaaa gttttataaa
    14341 tggtctgcat ttggttatac actttggatt attttgcccg ttgttttggg tatattttgc
    14401 accatgctta tacacgaaga aaaccagtat gatatgctca aacagctttg gattgtacct
    14461 attagtaaaa tgggatactt ttttagtaaa tttttcgttg ttttgattta ctctatttgt
    14521 tttatgctga ttacagccgt tgcttctgtg ttatttagtg tactttctgg atatgtcgta
    14581 tttagctggg caagcatttt atatttgttg aaaaaatgtc tagaaattgg tgtaatcact
    14641 gcttttgtaa tgttgccgat tttggcaatc gctacatctc aaaaaggata cattctccca
    14701 atttgtatca cattggtata tgccttttcc ggttttgtta taatgacaat aaatatgtat
    14761 ttacaccctt tatccagtat gggagttatt atcatgagaa ataaagacat tccaggtctt
    14821 atattttcac aggaaattaa tgtcccatta gcatttcttt gtatttgtat ttggagtatt
    14881 gtatcagtat tgtttgcaaa cattgcacta ggaagaagaa agtgaggtgt agataatgtg
    14941 gaaattactt tgggctgaat gccaaaaaat tcgtcgttca aaaattgttt ggattacagt
    15001 ttttgcatcc attatgatag cagtcatagt ttttgcagga gggcaggatg tatataatga
    15061 cccagattta cattatggct taaagagcgt ttatgacggt tcacggtata tagacaatgc
    15121 aggttggtat gtggacgcag tacaaccttg gtcaactttt tttgttcttc ctgctgtcat
    15181 tgccctatta ggaagctata ttatttgtcg tgaggaagag gaggacacta ttaaatccct
    15241 gcgtcttatt cccataaatg aaacaaaatt aaccatagca aaaatgatta ttacttttat
    15301 attcagtata cttctttatt tgttactctt tactataaca tttttgactg aatccgtttt
    15361 acatttttcc gatttatcga caaaactggt tttaagttgc atgaaagagt attttcttga
    15421 tggcataggt gtattctttg caatttctcc cattattgcc ttggtatcgc gcatgaaaaa
    15481 aggatattgg cttgcattag tattcacaga agtttattct attgctggac tatttgcggg
    15541 tatgtcaaat gtcttacaaa ccttttttcc tattacggca atatttaata tttctggata
    15601 ctatataact actggagaaa aaattatggg gagtatcata agtttattac tttgtgcctg
    15661 cctttctgca tttatactta aagggctaaa acacaatgaa aaaaattgaa ataggtatat
    15721 caaaatgaaa aagataaaaa aactgattat tctaattgta ttcttgtttc tttttggcat
    15781 gttacactgt tatactgaat ggaaattcaa caataaccca cctaaagggg taatagaaag
    15841 aataaatgag tggactaata agattggact ggattagtaa cttatgttaa ttatagtgaa
    15901 tgtaatatta gcaagttatt gtttcgtatt ttaatactat aactttaaat atttggtttt
    15961 aatcttccaa acgaatataa aaaaactgtc acagaggcaa catatttttg aatatttata
    16021 aagtggtagt tttgattttc aaagctgccg ccttttcttc tatataagca aacttatcct
    16081 caacaaagtg taaaacatgg tacattttaa gaaaaaagta ccacagacac aagcaataaa
    16141 agaaacgccg agaacctaca aaaacgtagt gcttatgcag atttacgacg ccttctattt
    16201 tctcaaccgt cttaatgacc ttgtacaaaa gaaatttttc taatattttt tataatctta
    16261 tcttgtgatg ttttttatgt ataaatgact taataacaaa tccaataaaa attatggata
    16321 ttggtataat tagaacgagg aatttataat cattataatg tgacattatt cttacccaac
    16381 tctcagaaaa aatatatcct atactgatta atgaagtatt ccatatagtt attcccacga
    16441 tagatgatat tacaaaagtt ataatatttt gacctgctat acctgctata aaagatatgt
    16501 atgttctaca taaagggatt aatctacata cacaaacaga aatagaagag tatttattaa
    16561 aataatcttg agatgcaaat attcctttct ttgcctttgg acatatctct attattttat
    16621 ttatgataga tttacctccc caatttccca ctgtataaca aaatatcgaa ccaagtatac
    16681 ctgcaattat acttaaaatt aaaattgtca gaaatccaaa atgatttctt gcagcaattg
    16741 ctccacataa aggtaaaaca acttcgctag gtaatggaaa acaagcatac tcaatcataa
    16801 ttaatacaaa tacagagatt aatccatatt gtaaaataaa actattgatt acagacactt
    16861 acttatctat ctcctttctt atttaaaggt tttaagggta catgttctaa ggattctcac
    16921 cctatattta aagaatcaat ctctttaaac tattatatgt atttacacaa agcatttata
    16981 acatacttta aaaatagtac cttaaaataa gaaacttaat aaataaaaaa agattacgtg
    17041 gctacgtcct actctcccag gcagtttccc accaagtacc atcagcgcta gagagcttaa
    17101 cttctgtgtt cggaatggga acaggtgtat cctctctgct attgtaacca cataatcttt
    17161 atgcttaata tttctattaa gtttttaata agtttataga a
//
LOCUS       DAEMIK010000090        16828 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEMIK010000090 DAEMIK010000000
VERSION     DAEMIK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.090"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            342..1715
                     /locus_tag="KPK87_002453"
     CDS             342..1715
                     /locus_tag="KPK87_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2538596.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1971..3038
                     /locus_tag="KPK87_002454"
     CDS             1971..3038
                     /locus_tag="KPK87_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG2538597.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3182..3490
                     /locus_tag="KPK87_002455"
     CDS             3182..3490
                     /locus_tag="KPK87_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG2538598.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIKS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3483..4913
                     /locus_tag="KPK87_002456"
     CDS             3483..4913
                     /locus_tag="KPK87_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG2538599.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4991..5365
                     /locus_tag="KPK87_002457"
     CDS             4991..5365
                     /locus_tag="KPK87_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG2538600.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5359..7563
                     /locus_tag="KPK87_002458"
     CDS             5359..7563
                     /locus_tag="KPK87_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG2538601.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7563..8972
                     /locus_tag="KPK87_002459"
     CDS             7563..8972
                     /locus_tag="KPK87_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG2538602.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8957..10021
                     /locus_tag="KPK87_002460"
     CDS             8957..10021
                     /locus_tag="KPK87_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG2538603.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            10051..11472
                     /gene="nhaC"
                     /locus_tag="KPK87_002461"
     CDS             10051..11472
                     /gene="nhaC"
                     /locus_tag="KPK87_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG2538604.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11901..12365
                     /locus_tag="KPK87_002462"
     CDS             11901..12365
                     /locus_tag="KPK87_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2538605.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVDFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12664..13026
                     /locus_tag="KPK87_002463"
     CDS             12664..13026
                     /locus_tag="KPK87_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2538606.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13621..14457
                     /locus_tag="KPK87_002464"
     CDS             13621..14457
                     /locus_tag="KPK87_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538607.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            14541..16655
                     /locus_tag="KPK87_002465"
     CDS             14541..16655
                     /locus_tag="KPK87_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBG2538608.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
BASE COUNT         6673 a         1846 c         3134 g         5175 t
ORIGIN      
        1 ttatattatg gactaaaaaa tccaatgagc taatttattg tttttttata tttgagagat
       61 tacaacttat tagtttgtat atctcttttt ttaataaaat caataatata taaaactatt
      121 ggcatattta atgacaattc aaatattgta tataaccata atgaaagata tataaataaa
      181 attttaaata tacttaaaaa tatattaata taatgtacat aaaaaatatc ataaatatat
      241 gataagataa atacaatagc aacattttat taattttttg ggtttttata tatttaggta
      301 ctggggtgat atagttgaac caagagatat tttttaaaat aatgaatgaa atattggaaa
      361 atattgatga aggggtacat tttgttaata aagacgggaa aactataatc tacaataatt
      421 ctgtacaaaa aatggaaaaa atgaacagac acgatatttt aaccaattca ttttttgata
      481 tagtcaacaa aatgaaaata aacaggagta cactattaga agttttggaa aaaagggaag
      541 ttatcaaaga caatattcaa aaatatctgg ataaaaatgg aaaagagata acagcaatta
      601 atactacaat accaattgat attaaaggcg agtttgtagg ggcacttgaa atatcaaaga
      661 atatgacaac gatagaaaat ttactaaata gaaattataa aaaaaattta gtcaataaag
      721 ttagtagtag acaaaaaaag ggttatgaat ttgatgatat tttaggggaa agtaatttga
      781 ttaaagaagc aatagaaaaa tctaagaaag cttgcaagtc tgatgcttca gtatttatat
      841 acggtgaaac tggaacgggt aaagagttat ttagccagtc cattcactat ggaagtaata
      901 ggagaaacta tccatttata gcacaaaatt gtgcagcttt gccggaatca ctattcgaag
      961 gtattctttt tggaactagc aagggaggat ttacaggagc aatagatagg ccaggcttat
     1021 ttgaacaagc taacaaaggg acattgttat tggatgagat aaattccatg cctattatgt
     1081 tacaggctaa actattaagg gttttacaag aaggttatgt aagaagggtt ggaggaacta
     1141 aggatatacc tatagatgta agaattattg caacgacaaa tgaaagtcca aaggtaattt
     1201 taaacgagca taaaatgcga aaagatttat attataggct taacataatt catataaaaa
     1261 ttcctcctct tagagataga aaagaggaca tacctctatt gtgcaaaaaa tttatttcaa
     1321 agtataataa aaagttaaat aaaaaagtgg taggaatttc agatgaagct attaaaattt
     1381 taaaaaatta cgattggcaa ggtaacataa gagagctaga aaacataatt tattgtacta
     1441 tgagtatgat ggaagatgag gtacaaatag aaacaagtat gattaatcta aacgactatt
     1501 acaatcttaa agagaataaa gagagtaaca attattcttt aaacgatttg gaggacaaaa
     1561 cacttagtaa gattatcagt gagatagaag aaaagtatat atatgaatca ttagaaaagg
     1621 catcttacaa tataactaaa gctgcatcaa ttttaggtat gaataggcaa aatttacagt
     1681 ataaaataaa aaaatataat atcaaaatat tataaataag caagataaaa aaacaaaatc
     1741 aaagtaaaat aaaaaagcaa aatagaggag caaaattatt ataaaagagc aaaatatttt
     1801 gctctttttt ttatttctca taagaaatat tattttttat ttatgaaaat acataataaa
     1861 ataattatag tttcaacgtt ccaataaata tttactttaa ttttaaagtt ggcacgattt
     1921 atgctttaac taattattga gaatatataa atttttagga gggtgtatat atgagaaaag
     1981 taagagtagg aatatgggga tttggagcta tgggaatagg tatggctaat atgattctaa
     2041 agaaagaagg tatagaaata gtatctgttt gtagtagaag tacttcaggg aaaagtatgt
     2101 atgatgtttt aggaattgaa agaggagaaa gaccagaagt tattatcaat aaaaattatg
     2161 aagaagtttt tagagaaaaa agtgtagatg ttgttttgtt agcaactgat tcatttacta
     2221 aaaaggcttt tgataaaata attttcttat taaatagaaa aataaatgtt atatcaacag
     2281 cagaacaaat ggcatatcca caggctgatg atgcagattt agctaaaaaa atggatgaag
     2341 ttgcaaagga aaatggagta agtatacttg gaacaggaat aaatccagga tttgtattag
     2401 atttactagt attggcactt tcaggtactt gtgaagaagt tactagtata aaagctaaga
     2461 gagtaaatga tttatctcca tttggaaagt ctgttatggt ggaacaaggt gttggagtaa
     2521 caagggaaga atttataaaa ggtgtagaag ataagactat agcaggtcat gtaggttttg
     2581 tagaatcaat aaatatgata gctgatggat taggttggaa attagataag atagaacaaa
     2641 caaaagagcc aataatgact acagtagata gaaaatctaa gtacggagag gcattggctg
     2701 gaaatgttgc tggttgtaga caatgtggat atggatatgt aaatggagaa gttttaattg
     2761 aaatggagca tccacaacag ataataccag aagcagaagg tattaaaaca ggagactatg
     2821 tatcaataaa aggtatacca aacattgatt tacaaataaa tcctgaaata cctggaggag
     2881 taggtactta tgcaatgata gtaaactcta ttccattaat aataaatgct agaccaggtc
     2941 ttaaaactat gttggatata ccagtaccta gagcaataat gggagatata agaaatcaga
     3001 tagaagttga attggaagaa gaatcaaagg ctaattagta agataacagc taactaatta
     3061 gtaagatagc aactagctaa ttagtaagat aacagttagc taattggtaa aataatgttt
     3121 aatttaaaat aacagtaaat taattagtaa ggcaatagtc aattaataag gggatgaaaa
     3181 aatggatgct aaaattaatg attgggttat tatacacaat atagtattga ctccagaaga
     3241 aagagctcca caagttccag aggatactaa gaaagtatcc ttagaaatgt gggttaaagg
     3301 ttttataaaa agtgatgctt ctataggcga cttagtagaa gttaaaacaa ttacaggtag
     3361 acttgtaaaa ggtaatttac ttaaagtaaa tccatattat actcatgatt atggtaaatg
     3421 tataccagag ttactccaaa taggaatcca agctaaagaa atattatttg gaggtgtata
     3481 taatgaataa ttcaacatca atgaaagata tgagttatca agctgttatg ggaagaaata
     3541 acgaaataat gaaaaatgca atcggattgg attattcttc ttttgaacaa gagggaatag
     3601 ggtttgatta tgagaaaatg atgagtgaaa caggatatac attacaagat attgaagcaa
     3661 ttcaatcaca gtatgctgtt ggaaatactc ctctaataga gcttaaaaat ttgactaaat
     3721 tagctagaaa atgtgctaaa gaaggtaaag gagctagaat atttgtaaaa gatgaagcaa
     3781 tgaatgcatc tggtagtttt aaagcaagaa gagcagctac tgcagtatat catgcaaaac
     3841 aaatgggata taaaggtgta atagcagcta ccagtggtaa ttatggtgca gctgtagcat
     3901 cacaagcggc aatgcaggga ttaaaatgta taatagttca agaatgttat gattctaatg
     3961 gtgtaggtca acctgaaatc atagaaaaag cgcgaaaatg tgaggcatat ggagcagaag
     4021 ttgttcagtt aactgttgga ccagagttat tttatacgtt cttagtttta ttagaagaaa
     4081 caggctattt taacgcatca ttatattcac catttggaat tgcaggtgta gaaactttag
     4141 gttatgagtt agctatccaa tttagagaaa agtataaaaa agaccctgat atagttgttt
     4201 gtacaaatgc aggtggtggt aatcttactg gaactgcaag aggattgata aaagctggtt
     4261 ctattaatac taaagtagta ggagcaagtg tagatttaaa aggattacac atggctagtg
     4321 ataatcaatt taataaaaaa tcatttacaa caggacatac aggttttggg ataccatact
     4381 gtacatggcc tgataggtca gatgtaccta ggtctgcagc tagaccactt agatatatgg
     4441 atagatatgt attagtaaaa caaggagaag ttttttatac aactgaatta ttggcacagc
     4501 tagaaggtat agaaagaggt ccagcaggaa atacatcttt agcagcagca ttctctttag
     4561 ctcaagagct agatgaggat aaagctatag tggtacaaga aacagaatat acgggtgcag
     4621 gtaaacatat atgtccacaa cttacatttg caagagaaaa tggaatagat ataaaatttg
     4681 gaaatccaag agaagaaatt gcaggagtaa acttaatatt accagaaaga ccagaattat
     4741 tgaaatgtgt tgatattgat atgaataaaa taagaaagtc atttataaaa aattgtatat
     4801 ctaataatca cattgataat attgataagc taagtaatcg agatatagaa tttttaatgg
     4861 aagaagtaaa aagttcaaga gattttgttg ttgatgtatt aaataattta tagtaattat
     4921 tgttttttaa tttaatataa aataaaaatt cagaaaattc gattaaatca atacgaatgg
     4981 aggaatgttc atgaaaaaaa gagaggatga ttttgaagtt agaagaaaac atcttcaaga
     5041 actgagtgat gatgagttaa aagaaaggtt ttggagttta gctacccaga tagtagaacc
     5101 aatgatagaa cttggtaaaa agaatacaac accatcaata gaaaggtctg tcttgttgag
     5161 aatgggattt tcttctcttg aagtaaaacc tattttagaa ggggttatgg aaagaggtct
     5221 tataggtaaa ggagcagggc atgtagttta taaattggct aaatctaaaa atataacggt
     5281 tagagaagca ggtttgttat tagttaaggg tgaatattgg gatgaagtta catgtttatt
     5341 tagagaaggg gtagaatcat gttaaaggaa aataaaaaat tggatataga ctatatatta
     5401 aaagatttgg ataaatataa accaaaacga agaggttggg tttggagaga acacttggaa
     5461 aatcttgaaa tgggaccttt taaatataaa gactgtacaa aacctttaaa gaagagtgta
     5521 gggctaccat catctaagta tttcaataat atagaccctc aaccaagtcc agttattact
     5581 actgaaatag cttctggtag atttgaggat gatataagaa gaatgagaat ggcagctcat
     5641 catggagctg accatctaat ggttatcaga actgctggac aatcacattt tgatgggctt
     5701 atagaaggaa ctccacaagg tataggtgga gttccaataa caagaaaaca agtaagagct
     5761 caaaggaagg ctcttgattt tatagaagaa gaagttggta gacctattaa ttaccattca
     5821 tatgtaagtg gtgttgctgg tcctgaagta gcagtaatgt ttgctgagga aggagtcaat
     5881 ggagcacatc aagacccaca atataatgtt ctgtatagga atataaacat ggtaaggtct
     5941 tttgtagatg cttgtgaggc aaagaaaata atggcttttg ccaatatagc tcaaattgat
     6001 ggagcacata atgctaatgc tactgctaga gaagcatgga aggtaatgcc tgaacttatg
     6061 gttcaacacg ctttaaactc tatattctca gaaaaaatag gtatagataa aagtaatata
     6121 tgcttatcaa cagttccacc aacagcacct ccagcaccat gtttaaaaat agacttacct
     6181 tacgctgttg cacttagaga gttatttagt gattataaga tgagagctca aatgaatact
     6241 aaatatatgg aatcatcaac tagagaagca acagttactc atgtattaaa cctattaaca
     6301 tcagtattaa ctagagctga catacaatca actataacac ctgatgaagg tagaaatgta
     6361 ccttggcata tatacaatat agaagcatgt gatacagcaa agcaagcttt agttggtatg
     6421 gatgggctta tggatatgat agaacttaaa gatgtcggtg aattaagaga caaagcaaga
     6481 gaacttaaag aaagagctgt attatatatg gaagaaatat ctgaagttgg tggatatttt
     6541 gaatctgtgg aacaaggatt ttttgttgat tctggaaatt atccagaaag aaatggtgat
     6601 ggaatatcta gaaaaataaa aggtggagtt ggtgaaggta ctgtttatga aagagaagaa
     6661 gattatttag cacctgtaac tgctcacttt ggatacaata atgtagctca atatgatgaa
     6721 aatgctatag ataatccatc aatgttaata gatggatgta catttgaaaa tccagacaaa
     6781 ataatatata ttgatgagct tgacgatttt gataatgtag aaaatagact aaaagaatca
     6841 tatgagtaca gaaatggaac taaaattaag cctgaaatgg aatggtgtgc agatggaatt
     6901 gtaatgataa caatgatgct tcctactgat aaaagaacag cagagtttgc agctttggaa
     6961 tttgtcaaaa aaatgaactt acaagaaatt gaggtcataa gtagagaagt tatgcatgaa
     7021 tctgaaggga ctagaataga agtgaaagga agagttccgt ttgatataga tttaaacaat
     7081 ttagtaatac cagaagaacc taaggtacta actgatgaag aaataagagc agatatagaa
     7141 gaaaaaccta tgaaaatagt ttcagctacg gttggagaag atgaacattc agttggttta
     7201 agagaaataa tagatataaa acatggaggt attgaaaaat atggcataga atgccactat
     7261 ctagggactt ctgtacctgt agaaaaattg gtagatgcag cgattgaact gaatgcagat
     7321 gcaatacttg catctacaat aataagtcat gatgatattc actataaaaa catgaaaaaa
     7381 ttacacgatt attgtgtaga aaaaggaata agagataaag taatgattgc gtgtggagga
     7441 acacaagtaa cacctgaaat agctgttgaa caaggcatag atgctggatt tggaagaaac
     7501 agcaaaggca tacatgtagc cactttctta gttgaaaaaa gaagagaaat gagtaataaa
     7561 taatggaaaa tagcaaagtt tctttaaaaa tagatgtgtt ggtagctgaa attggaagta
     7621 ctactacagt ggtaaatgct ttccacgata ttaatactaa caatcctata tttttgggtc
     7681 aaggtcaagc tccaacaaca gtgtttgaag gtggcgatgt aagaaatggc ttatcaggtg
     7741 ctattaaaga tttagctaat aaattatctg tagatgatat agagtataat gatatgtttg
     7801 caacttctag tgcagctggt ggattaaaaa tgactgttca tggattagtc tatgatatga
     7861 cagtaaaagc agctaaagaa gcagctttag gggctggagc tgttattcgt caaataacat
     7921 caggtagaat taaaagaact gatttaaata aaataaaaga aataaaccca aacataattt
     7981 taatagctgg tggagtcgat tatggagaaa gagatacagc tatttataat gcagaaatga
     8041 tagcttctat gaatctaggc ataccagtta tatatgctgg taatgttgaa aatcaagagg
     8101 aaataaggtt aatatttgaa gatactaatt ataagcttta tataactgaa aatgtatacc
     8161 ctaaaataga tttattgaat atagaaccta caagaagaat cattcaaagt gtatttgaag
     8221 aacacataac tacagcacca ggaatgaaat acattaaaga aatggttaat gaaaatatta
     8281 ctccaacccc aggagctgta atggaagctt ctaaactttt atataaaaat attggagatt
     8341 tattaactct ggatgtaggt ggagcaacta ctgatgtaca ttctgttaca gatggaagtg
     8401 actatattaa taaaattttg gttaatcctg aacctactgc aaagagaagt gttgaggggg
     8461 atttaggtgt atatgtaaat atgaagaata tagtagaagt tataggtaaa gaaaacttac
     8521 aaagtgaact atctatagat attgatgcag taatagagaa ttatcctcca ataccaaaat
     8581 caaaagaaga aatattattt gttgagaggt tgacaaaaga agctgttgtt aaagctatgc
     8641 taagacattc aggaaaaata agaaatatct atggaacaac tggaaaagtc aaaattgcag
     8701 aagggaaaga tttaacagaa gtaagatata tagttggtac agggggagca cttactaggc
     8761 tccctagtag aattgaaata ttagataata tgctcaaata caataaaaat aatgaattac
     8821 tatttccaaa ggaaaaaaca aagatattaa tagataatga ctatattatg gcatctatgg
     8881 gtgttttatc taaaaaatat gaagaagcat ctcttaagct cctgttaaaa agtttgaatt
     8941 ttgaggagga aagattatgt atcctaggtt agaaatagat atagaaaagt taaagcataa
     9001 tgcaaaatta atttcgcaga tgtgtcatga aagaaatatt aagatatctt ttgttactaa
     9061 atcattttgt gcacaaaaag aaatagtaga agaaatatgt agtgaaggaa tagaccatat
     9121 agcagattca agaatacaaa acttaaaaaa tttacaagat ataaatctac ctaagatatt
     9181 gattagaatc ccaatgttaa gtgaattaga agaagttatc aattattgcg atattagttt
     9241 taactcagaa ttaggaacaa taaagaaatt aaatgaatta tgtgaaagta aaaatattat
     9301 tcataagata gttcttatgt ttgacttagg cgatttacga gaaggatact tttatgaaga
     9361 agactttttt aataatgtaa gagagatagt aagattaaaa aatattgaaa taattggtat
     9421 agctacaaac ttaacctgtt atggagctat tataccatct agggaaaata taggcagact
     9481 ggtaagtata gcaaaaagaa tggaagaaaa atttggaata aatctagaaa tagtatctgg
     9541 aggtaattca agttctattc atctgttgat aaataataat atgccagaag gtattactaa
     9601 tttgagaata ggcgagtcca ttctacttgg tagagaaacg gcatacggtg aaaatataaa
     9661 tggaacatat caagatgcat ttaaattaat atgccaagta gttgaatgta aagaaaaacc
     9721 atcagttcct attggagaaa taggagttga tgcctttaga aataaacctg tttatgaaga
     9781 taagggaatc ttaaaaaggg caatcatagc tataggtaaa caagatataa atattgactc
     9841 tttaattcca atagacactg atataaaaat attaggggca agttcagacc acatgatact
     9901 agatgtaagt aacacaaaat atgattataa attaggtgac aatttagaat ttttattaac
     9961 ttatgggggt atcatgtcat ctagtacgag caaatatgtt tcaaaaaaaa ttatagtgta
    10021 gtgtttaaat aattaggagg aaactatttt atgaaagagc gagaatgtag aaaagcaagt
    10081 ctgtttgatg caatgatacc aattacttgt ttgatattat ttttaagtct aggagtatta
    10141 gtgtatggtt catcacctca tgtaccacta ataggagcag cagcagtagc aggacttgtg
    10201 gctgtatata gacttggatt taaatggaaa gagttagaat taagtatgtt taatagtata
    10261 aagatggcaa tgcaagcaat cttaatcata attataatag gcgtacttat aggaacttgg
    10321 atagtaagtg gagtagttcc atgtatgatt tactggggac taaaaatttt atcaccaaat
    10381 atttttttag tagcatcaac attagtctgt gcaatagtat ctcttgcgac aggttcatct
    10441 tggagcacaa tgggtacagt aggagtagct cttttaggaa ttggtcaaag tttaggtatg
    10501 ccaattggat taatcgtagg ttctataata tctggagctt actttggaga taaactatca
    10561 cctttatctg aaacgactaa cttagctcca gcaatggcag gaacagattt gtttacacat
    10621 ataaaatata tgctatattc gactatacca tcattattaa tttgtttagt aatttatggt
    10681 gtaattggaa tgaagtattc aggtcaagca ctagatataa aacaaataga attaattagg
    10741 tctacattag attctacatt taatacttta tcaccagtat tattattagc tccagcaata
    10801 gtaattggat tagttgtatt taaagttcct gctataccag ggcttatagt aggtgctgta
    10861 ttgggagtat tgtttgcagt agtttttcaa ggagaatcta tgaatacgat attagatgca
    10921 gcacaaaatg gatatgtttc gtcaacacaa atacaagagg tagatgcttt attatctaaa
    10981 ggtgggatta tgaatatgat gtccactgta tccttaacaa tatgcgcttt atctcttgga
    11041 ggtattttag aaaaaacagg tatgcttgaa gtagtagcgt cttctttact aagattagca
    11101 aagggtgtat ttggaacagt attatgtact atggtaactt gtacaattac caatataata
    11161 gcaggtgaac aatatctatc tatagttata ccaggaagaa tgtataataa ggaatataaa
    11221 aagagaggta tacatcctaa gatgttgtcg agagctttag aagatagtgg aactttaacc
    11281 tctccattag taccatggaa tacatgcggg gcgtatataa cagcaacatt aggtgtatct
    11341 gcactaacat atggtccata cgctttatta aatattataa atccaatagt atctttagta
    11401 ttaattgcat gtaagtttaa aatagcaaga atagaagatg aacctgaaac ttgtttaaat
    11461 tttgatgttt gataatagat aacttatgta agaacgtagg aactaggggg gtagcctgtg
    11521 gggaattagt aagacatatt ttagtgtgcg aaattctata aatcagtaat cttgtgactt
    11581 tagccatggg aggttcagat tagtaaattt atagttttat attagtctat attttgatct
    11641 tttaattgtt tgtataaatt ttttatagaa aaagatttct tttagacttg cctaaaaatt
    11701 aggcaagtct tttaagtatg ttctgattat aagagcactg tgtgacaaga atgatgagat
    11761 tatttttgct atactttcga tataagaaat ggaaggaggt tatgttttat attcatatga
    11821 aataattagt tacacagtaa attttcgtat aatttggtat aaaaagatta gaaatgaaaa
    11881 taaagtaatg gaggaataaa atgactagaa tatccaatat aacaatagct gaacaatctg
    11941 agtactgttt tttggcaata cgaaaaacaa ttgactttat ggtagatttt tcagaatttt
    12001 caaaacaaag ttttgaaaaa atttctaaat atctggagga gagaggaatt ttatcttcag
    12061 gagcaccgat tgtatgtttt cataacatgg atttagcaaa attggatgtt gaagtagggt
    12121 ttcctgtagc cacttatata gatgggaaaa atgagattct acaaaggatt gttcctgcac
    12181 agaaaataat aactgcaatt gatttaggac cttatgaatt acaagaccct actttagaag
    12241 acttattttc atgggcaaat agcaatggat ataaacttca tggcgatata tattaccagt
    12301 atttaaatga tatcaatcgt ccagctagtg aatatcttac gaaaatgatg ttacccatta
    12361 tatagaaagt taaagattat cgtttgttag ttgataatta gaagaacatt tactgctact
    12421 acataaattt tatcctaaat aatatcaagg tgatttaatt tatgtattct aatgtaactg
    12481 tagataatca atttataact aacttagtgt aatttagatt taatttatgt attataatgt
    12541 aaccccaata tattgttaag gtctacttaa gatgctcaaa aggctatgct taatttattg
    12601 ttatataatt tgtagagtgg tataatttag aggtaatatt atatagatat atagggggaa
    12661 aatatgaaaa tatatgatat attaacacgt ttttatattc aagatataga aaaagccata
    12721 cctttttatg agaatctttt aaaagaaaaa tgttctttgc gtttttccta taaagaagtt
    12781 gggttggaat tagcgcaaat aggaaatgtt ttactattat ctggttctga tgatgctctt
    12841 aaaccattta tagaaacaaa atctactttt atggttgatt ctgttgatga atggagaagt
    12901 tatctattgg ataatggagc agttgtagta agagataaga aaaaagttcc tacaggatat
    12961 aatatgacat tgagacatcc agatggtaca attatagaat atgttcaaca tacaaagcag
    13021 gattagttaa aaatagattt tactactgag caactaatta gaatctattc ttagatgtac
    13081 aatttattat ttttggtatt aatagattat aagtcaaatt ataatatttc aaaatagaat
    13141 agaaatttat ttacataaaa gagagtgttt aaaaatgaac tttttagttc atgaagcact
    13201 cttttttgta tggaatcaaa tacatcaata tataaaataa actgatgaag agcttaaatt
    13261 aaaataaaaa gattcataac aggaccccat taaactttaa gatagggtaa gtttgtaatc
    13321 atattaaata attgtaaaaa gctgttttta gtcgtataat ataaatattt atcatttaat
    13381 taaggaatat aaaaggaatc taataagaag aaaataataa ataacttttt agatttacta
    13441 aatgaaaagt tattgaaaat taaaggatta gagtttttaa tcagaaagta aaaagtaata
    13501 tattaaattt atgaattgaa aacttaaaca acaaatttgt ttataaaata gcaagattca
    13561 ttaaatataa ctaagtaata attgatataa tgttgcatgg ataaaaggag ggaagctgca
    13621 atgtcttaca aagtaattaa tgtcataact gctattgact atatagagga gcatttatca
    13681 gaaaagctgg atttagatat agttgctaat gctgttcatt actcaaaata tcacctgcat
    13741 agaactttca caacttctgt tggtctaaca atgcatgatt acataaagcg gagaaagttg
    13801 acagaagcag caaaattatt ggtattttca aagaaaccaa ttattgaaat tgcattgata
    13861 gctggatatg aaagtcaaca ggcattcact tctatattta aagctatgta taaaaaatct
    13921 ccaaataaat ataggaaaga acaagaattt tatcctttgc agctaagatt tgttttgaaa
    13981 aaagataacc tattttcaaa gaatgtacta gagttggaaa aagaaattaa acttgcaagt
    14041 atgtctgaca taccattatg gatgaattta gtgagattag tcatagatgg atttccaaat
    14101 ttgcaagagg aagaatatat atatcaatta gaacagtata ttttagaaga aagagcttta
    14161 atattaaaac ttaataatgt cgctattgca aatatggtgt ttaatagaga aactagaagt
    14221 attgattttt ttggaataca tccacagtat cgtaattcag acattgcaca agtgtttttg
    14281 aagaaagtta ttgaagattt cttgatagat acagatattt caattactac ctttcgagag
    14341 ggtgacaaag ctgatacagg acatagagat atgataaaga agttggggtt tgcagaagca
    14401 gagttgcttg tggaatttgg atatcctact caaaaattta ttcttccttt aagttaaata
    14461 gcaagaatga ttaaaaactc caaaacacaa ttaagtataa tgattcagtg taagtactta
    14521 attaacaaag gaggatttaa atgtctgaaa ataaacaagt agtaaattgc gatattattg
    14581 catctaattc taaacataat atgtgtgaat atgatgcaga gttattaact ttttttccag
    14641 atgagattgc acatctaata gagataaata acaagctaga caatgctttt aagaaagctg
    14701 aaaatcttgt tgacaagctt gataaggatt atatggacgc taaaatgtat atggtaaaaa
    14761 atcgtggtga aatagaccca catgaaatgt ttcaaaatga acaaggacta aaacaaatag
    14821 ataactatgg ggcttttatg gtaaaagttc gagataagat taataaaatc aaagattctc
    14881 catactttgc tagaatagat tttaggctga aagatatgga tgatgaatct aagtattata
    14941 ttggtagatt tgcatttgat tatgaagatg agcttataat acttgactgg cgttctccaa
    15001 ttgccagtat gttttatgat tatgaaatcg gaaaagctgg atatgatgct ccaataggat
    15061 gggtggatgg agaaataaca agaaaacgac aatttaaaat taaaaatgga aaattagaat
    15121 atgcactaga aagttccatt aatatacaag atgatattct tcaaaaagaa cttagtcaca
    15181 cttcagatga aaaaatgaag tcaatcattt caacaataca aaaggaacaa aatcaaatta
    15241 ttagaaatga taaagctgat acactgatta ttcaaggggt agctggttct gggaaaacat
    15301 ctatagcatt acacagaatt gcttttcttc tttatcgatt taaagataaa atatcagcaa
    15361 ataatgtaat tatcctatca cctaataaag tctttggaga ctatatttcc aatgtccttc
    15421 ctgagcttgg agaagagcca ttatgtgaat taagttttga aaatatagca gaggtacaat
    15481 tagatagagt tattaatttt gaaagtgaga aagacccatt agaaataaat gatgctaaat
    15541 ggtctgaaag agtgcgtttt aaatctacac ttgattttgt aaaacttatt gatgattata
    15601 taaaacaaat gccaaataag atttttattc caaaagacta cacttttgga agttttacag
    15661 ctaaaagtga ctggatacag agtagatttg aggcatataa cagatatcca gttaagaaga
    15721 gattagagaa ggtagctgaa gacattcatt ataaatttga atcagataat attatggaag
    15781 aagatttacc aaaggtaaaa agtatactta aaagtttgaa tggaatgttg acaataaaaa
    15841 atactcttac actatataag gatttcttta aacaaatgaa tatatcaaac atgtttgtga
    15901 tggcagaaaa gaaaacactt gaatggtcag atgtataccc atttatatat attcatgctg
    15961 catatgaagg tatacaagaa gataagatta taaggcatat tgttattgat gaaatgcagg
    16021 actatacacc tattcaatat gctgtaataa acttactttt taagtgtaaa aagactattc
    16081 ttggagattt tggacaattg gttaatccta atcacactca tacactagat gatatgaaac
    16141 agctatataa tgatggggaa ctagttatgt tgaataagag ttaccgttct acttttgaga
    16201 ttattaattt cgccaaaaag attcaagaca tatcttcatt agaacctatt gaacgacatg
    16261 gtgaagaacc agctttatta aaatgtaata acgagcaaga tgaaataaat aagataaaaa
    16321 tagaaattga agaatttaaa aagagtgata atgcgactct tggtattata ttaaagacag
    16381 ataatgacgc taaagctgta tatgatgcgt taagagaaga atatagtgtt cacttaatat
    16441 cttctgaaag ttctagcttt acaaaaggag tatccataac ttctattaaa atgtcaaaag
    16501 gtttagagtt tgatgaagtt attgtacctt cagtaaataa taaaacatat tatagtgatt
    16561 atgaccgcag tttgttatat attgcttgta cacgagctat gcataaactt aagttgacat
    16621 atactggtga attgactcaa cttatagata tgtagattat atacttatta tgtaagtgtt
    16681 tacttatata aatataacct gtcaataaaa ttcattaata aatcaataaa aacaatattt
    16741 gtaaaagtaa aagctataaa ttatatatta aattttataa tttatagctt ttataatact
    16801 gcttatctta atcctaccaa tagcaaaa
//
LOCUS       DAEMIK010000091        16660 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEMIK010000091 DAEMIK010000000
VERSION     DAEMIK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.091"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1056)
                     /locus_tag="KPK87_002466"
     CDS             complement(<1..1056)
                     /locus_tag="KPK87_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2538609.1"
                     /translation="MNTFEQLKISSTIIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKEL"
     gene            1309..2016
                     /locus_tag="KPK87_002467"
     CDS             1309..2016
                     /locus_tag="KPK87_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538610.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            2063..3658
                     /locus_tag="KPK87_002468"
     CDS             2063..3658
                     /locus_tag="KPK87_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538611.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKKINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNVYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            3762..4844
                     /locus_tag="KPK87_002469"
     CDS             3762..4844
                     /locus_tag="KPK87_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2538612.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            4993..5154
                     /locus_tag="KPK87_002470"
     CDS             4993..5154
                     /locus_tag="KPK87_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG2538613.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            5495..6763
                     /locus_tag="KPK87_002471"
     CDS             5495..6763
                     /locus_tag="KPK87_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG2538614.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            6958..7746
                     /locus_tag="KPK87_002472"
     CDS             6958..7746
                     /locus_tag="KPK87_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2538615.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRILETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            7746..8195
                     /locus_tag="KPK87_002473"
     CDS             7746..8195
                     /locus_tag="KPK87_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG2538616.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            8199..10463
                     /locus_tag="KPK87_002474"
     CDS             8199..10463
                     /locus_tag="KPK87_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2538617.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPESSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            10603..10914
                     /locus_tag="KPK87_002475"
     CDS             10603..10914
                     /locus_tag="KPK87_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538618.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            11200..11700
                     /locus_tag="KPK87_002476"
     CDS             11200..11700
                     /locus_tag="KPK87_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG2538619.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            11757..12743
                     /locus_tag="KPK87_002477"
     CDS             11757..12743
                     /locus_tag="KPK87_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2538620.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            12793..13515
                     /locus_tag="KPK87_002478"
     CDS             12793..13515
                     /locus_tag="KPK87_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG2538621.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            13544..15478
                     /locus_tag="KPK87_002479"
     CDS             13544..15478
                     /locus_tag="KPK87_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2538622.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            15471..>16660
                     /locus_tag="KPK87_002480"
     CDS             15471..>16660
                     /locus_tag="KPK87_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG2538623.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFI"
BASE COUNT         6473 a         1616 c         2818 g         5753 t
ORIGIN      
        1 taattctttt tttgttatta ttgagattgt gttaccactt cgatttcctc tagcagttcg
       61 accacatcta tgcaggtatt catttttact ttttggaaaa tccaagttaa aaacatgact
      121 tacatccaca atatctagtc cacgagcaga taaatcagtt gtaattaaaa tctttgcttt
      181 accaagttta aatttattta ttgcattctt tctatcttct ttttccatat ttccaaaaat
      241 tcctattgct ttgtaattat gataatttag ttttgatact aggacttcta tatttttttc
      301 attatttaca aatacaatag ctttttttgg atttgtagct gctaatgcct ttctaagaaa
      361 agtaaattta tcccttatct cacctaataa atatgagtga ttgatgttag gatttatttg
      421 tgatttttcc tttgttttta ttatttcaaa ctccttcata agtttatcac aaatttttat
      481 agtgctatca gtaagacttg cagagcatcc tataatttgt ctatctctta acgttgttct
      541 tattatgtct tctatacaag ttatattttt tccattcaat agattgtcca cttcatctaa
      601 gacaatagtt tttatattat gtgattttag tttcttttgt tttatcaagt ctaaaactct
      661 tccacaagag ccaattacta tgtgtggttt tactgctttt atatttttta tttgtttttg
      721 tatattgacc tccccaatta aagctaaaga agtaacggag agttctgcat ttttagccaa
      781 taattcaacc tggtttgtaa tctgcattac taattcatgc gtaggtgcaa gtataagagc
      841 ttgtgtttct ctttttgatg tatcaatttt ttcaaatata ggtaaaaggt acgctagtgt
      901 ttttcctgtt ccagtctgtg aattaatcaa taaatcctta ttttgaatta tattccctat
      961 aacaagtgac tgaacttctg ttggagaagt tatgtcttgt tttttgagtc catctattat
     1021 agtggaactt atttttaatt gttcaaatgt attcatattt tactccttat attttaataa
     1081 ttatagtcaa ctatttttta ttatacattg tttattcaaa tcgaactata tattctttta
     1141 taattatgtg taatttgaaa gtaaatatca attatattta tgcctttata tttctgatac
     1201 ctttgcattt tatagatttc taaaataaac aaataaaagt tgtaattgtt tgatatttgt
     1261 tatatattat atataacaaa tatcaaaact ataatatgga gggaaattat gttaaaagta
     1321 atcaatgtat ctaaatcata taaaaataaa aaagtagtaa gtagtgtctc atttgatgta
     1381 aatgatggag agctttttgg gtttatagga cataatggag caggtaaaac tactactatt
     1441 aaagcaattg ttggaataca cgattttgaa gagggagagg tactaataaa ttctaaatct
     1501 ataaaaaaac agcctgttga atgtaaaaag gaaatggcat atatacctga taatccagat
     1561 ttatacgagt cattaactgg tatacaatac cttaatttta tagctgacat atttgaagtt
     1621 gccaaagata aaagagaaga gttaataaaa tattattcaa ataagtttga actcaataaa
     1681 gcattaggag atttaatatc ttcatattca catggtatga aacaaaaatt agctataatt
     1741 tcggctttga tacacagccc aaaaatctta attcttgatg aaccttttgt aggtcttgac
     1801 ccaaaagctt cctttatatt gaaggaaatt atgagggagt tttgtgacaa aggtggttgt
     1861 atatttttct caacacacgt tttagaagtt gctgaaaaaa tttgtgataa gattgccata
     1921 ataaaagatg gaaatataat tgcttatgga actacagaag aagttaaggg aagtaattca
     1981 cttgaaagta tctttatgga attaatagag aaataaaatg ttttttatac aatatagtgt
     2041 ctaaagtgag gaatgataaa atatgagcaa tctaagtata ttaataaaaa ataatataat
     2101 aaatgaattt aaattaaatg cgttaaaaaa agctgatggt agagaaaaac aaaaaataat
     2161 gggaatgatg ctttcagttg ttataatagc gggtctatta attttttatg taacgagctt
     2221 aagtctctct ttagttgata tcttaaagaa aataaatcaa ctggaaatgc tactgatatt
     2281 tggttttgga ttatcaacat taacaactat tgttacatca ttatataaaa cgtcatcata
     2341 tctatttcaa gcaaaagatt tagacctttt gactagttta cctataaaag aatctactgt
     2401 actagcaagt aagatattaa tgttggttgg agcaaactat ttattttctt caatatgtat
     2461 gattatacct gcagtggttt actttttaaa ttctgatgta agtataactt atttccctta
     2521 tttgatattg ctatttttag ctttaccatt attaccgata gttgcttcat caataatttt
     2581 tctatttata gggcagatat catcaagaat aaaatacaaa aatttagtat taatcatagg
     2641 tagcattata ttgatgttgc tttataccct acttatgtca agacttaatt ctatgtcagt
     2701 tgaaatgcta aagaatagtg tgtctatttc agagacgatt agaaaagtat atcctcctat
     2761 atattatttt gttgatgctc ttaaaacagg aagtatagtt tcatttttag catttatagc
     2821 catctcaata ataccatttt ttatgtttgt aactctgttt gcaaaaaaat ttaaagatat
     2881 aaatgctaag atgagtgaag gatataagtc aaaaaattat gaatttaagg gtcaaaaaac
     2941 ttcaagacct tcaaaagctt tattaaaaaa agaatttaag agatatactt cttcgtttat
     3001 atacttttta aattcatctg caggtttgtt atttctaata gtaggtacaa ttttaatatt
     3061 attatttgga gcagataaag tgggtgagtt actgaaattg aatatagatt ttaatcttat
     3121 aaaaatacaa ttattgggag tattcttctt tataatagta atgaattgta caacatactg
     3181 ttcaatttct cttgaaggaa aaaacttatg gatactgaaa tcttctccaa tagatgaaag
     3241 agaaatattc tggtcaaaaa tattgatgaa ttttttattg aatgccccat tatctatttt
     3301 atgtctaata ttagtatcta taagattaga ctttgaaatt cagtttatta ttgtatcaat
     3361 ctgtttgatt gtaagttttt caatgtttgt agcattgatg ggattattat caaatttatt
     3421 atacccaaac cttgattgga aaaatgaggt ggctgtagta aaaagaagtg caagtatgat
     3481 agttacaatg ttagtatcat tagcttatgt aattatactt ggaggtatat atgtattact
     3541 tggaatttcg tttttaaatg tatatattat tttggcaagt atacttactt tgatattaga
     3601 ttttatgata tggagaatta tttctactaa aggagtaaaa atatttaaaa atttataggt
     3661 gtatttatgt caaataaaga cttatctttt ttagtcttgc aaataatagg ttaagtagtt
     3721 atactatgta tagtgatatg aaaaaattta ggagaaaata aatgagaaaa ttatccaaaa
     3781 gagaaaaaga ttttattcaa tatggatttt tattattttt gatatgtatt actacttatt
     3841 tagtattaac tactttagat ataaaactaa tacctaaaat tataaggcta gtaaataaga
     3901 cttatatatt tcttggcata ttgacaatta tagtatatat gataattgaa tctattgtaa
     3961 ctcatttgat aataaatgca gttcaaaaaa caaaagtaaa atttattggt attaaaatgg
     4021 ctacaatggg attttactat aacttagtta ctccatttgc atctgggagt caaccaatgc
     4081 aaatatatgc acttacaaaa tatgatgtaa gccttagtaa atcagtggct atagttacta
     4141 ataaaactgt agtatttcaa agtacagtta ctgtattttg tggaatttta attttgctaa
     4201 ataagtcatt actgatggag caagtacatt ctgtgaaagt cttgataact tttggaatga
     4261 ttatgaatgt aagtatgttg ttaggtggac tactaatagt atttagtcca aaacaagtaa
     4321 aaatgatagc agaagttttg attaattttt tatctaaatt taaaacattt aaatttttag
     4381 attcaaaacg tgatagaata aatcattata ttgatgatta taattattct ataaaaatat
     4441 ttattaaaaa tgtaagaatc ttagttttaa gtatattact tactgtgtta caattaatcg
     4501 catatttcag tatagtttat tgtgtgtata aagcctcaaa tttaagagga gtttcatatg
     4561 tacatataat gacattacag gtctttttat atatggctat atcaccaatt ccaacaccag
     4621 gaaatgttgg agctaatgag gttacatttt ttactatgtt ctccaatata attccaaaag
     4681 aattattggg gtactctgta ctcttatata gtgggtttgt atactatttt atattaatag
     4741 tttcaggttt atttacattt ggaacgcatt atacattaaa ttcttggaaa aaggaaaata
     4801 aagtaaattc taatttagat aaggttattg aaaaattaaa ttaataaaat aattagggaa
     4861 attctatata taaatatggt gttgaataag ctatattata ttgagatatt tccctttttt
     4921 gattaaaatt atagaaattt gaaataataa gtaataaaca gaaaaagtga tattgttttg
     4981 aaaggagaaa ctatgaaaca agataaacaa aatgaaaaac aaagtgagat ttctaagata
     5041 aaacaaagca atgataataa acttgaaata gctcaagaat tacatgtaag taaaaatata
     5101 gggaatatgc ttggatatag aatagataag aaaaaaatga atagaccaag ttaatgaaaa
     5161 tgttgaaaat gggacatttt aaaaaatata tataatgaaa tgttttattg ttaaatttaa
     5221 acttatttta atgaagtaaa aagtcgaaaa atatctaaaa agatatggat ataaaaaata
     5281 ttaatatata tatactataa acttgacaaa atcaaccttt taataactag caaaaaatag
     5341 caaaattata taagcgttat ttaattgtca aaaatgaaag ctaaattgca tgtcctaaaa
     5401 aaaatgattg ataacattta tttgtgtgct ataattatat agtaaaacaa taagtatatt
     5461 gcataatgta tgcaaatcgg gaggagagac ggacatggaa gcatctatac acacaattgc
     5521 cagcaataat ttattgataa tttttgctat tgtaagtata actggaataa tttgtagtaa
     5581 gcttagtgaa ataataaagg ttccagatgt agtgttatat ttacttgtag gtattttaat
     5641 aggaccatca tttttaaaat ttatagatat tcgtggattt caaatagaaa atcaactaat
     5701 acttacattt ggttctgcat ttattttata tttaggtgga aaagaaataa gtttaaaagt
     5761 attgagaaat gtgaagatat ctgtattctt attagctact ttaggagttg tgatatctgc
     5821 ttttataatg caacaagtaa taggatttac ttttgggata agtgcaatga ctgctttact
     5881 agcaggaact ataatagcat caacagaccc agccactctt gtacctatat ttaatcaggt
     5941 aaagataaag gatagggtaa agcaaactgt aataagtgaa tcagctttta atgatgcaac
     6001 aggagctata ctaacatcag cagtgttggc tgtaattttg tcaaataaat tttcattagg
     6061 tgaaaatatt tatgaactgg ggacaatggt tattgtcgga gttttagttg gtttaattac
     6121 aggaatactg cttttgaaat tagttaatga taaaccttat ggaatattta aagattttgc
     6181 accaataata tctgttattt cagttgtagt tgcatatgaa ttatctacaa aattaggtgg
     6241 aagtggatat atgtcttgct ttattgtagg tataataact ggtaacaaga aaaactttaa
     6301 aatttggtta agtcaaaaat cttatgatgc agatttttat gttgctgaaa ctttaggtac
     6361 tatttgtcgt atggcaatat ttataatatt gggtagccaa gttaatttgg ctcagttaag
     6421 taaatatttt atgccatctg taattacagt attagtatta atgtttatag caagaccact
     6481 ttgcgtttta gtttgtacac ttgtagacag ggaagcaaaa tggagtaaaa gtgaaatatt
     6541 atttatgatg tgggtaaggg aaacaggcgt aataccagca gcactttgtg gtattatttc
     6601 agcaatgaaa gttcctggat acgaagtgat ttcatctgta gtattcatga ctatactaat
     6661 tacattagta atacaaggaa gtactacaaa attagtagct aaaaaacttg gattattaga
     6721 agaagaagtg ataaatataa gtgaaaaagt atcaactttt taaagttttg ctttaaaata
     6781 gtacctgtta atggctattt taatattgtc taatgatgat tattgaaata gtcatttttt
     6841 tatgtataag taatttacag aatttacata ttagacatat ttattatcaa aatttgttga
     6901 agctatgata taattagttg tatttaaaat aagaatagca aataagggag gaaatttatg
     6961 tttacggtta tggatattgt acagccagat acagtagaag aagcgtactc aattttaaat
     7021 aaaagaaaaa ctaaccaagt aattggtgga agtgcttttt taagaatggg gaaaaaaaga
     7081 ataggaacag gaatagaatt atctaaatta aatttagatt atataaaaga gtatgaagat
     7141 tacgttgaga ttggttctat gactactttt agaatattag aaacaagttc cataataaaa
     7201 aataattttg gaagaataat agaagattca gttaaaaata taataggagt tcagtttaga
     7261 aatgtagtaa cagttggtgc aacagtattt tctaaatatg gtttttctga cttgatagta
     7321 gcattacttt cattagatac agaagtagaa ttatataata ttgggagaat tagtcttgaa
     7381 gagtttctaa atagagatta tgagaaagat ttacttatta agatatacat aaagaagaca
     7441 aataagaatg cttcttataa atcacttaga aatgcaaaga gtgattatcc aattctaaat
     7501 gtatctgtat caaaacatat ggaacaattt aagttatgtg taggagcaag accgcagaaa
     7561 gctacgattg caaaacaagc tagtgaattt ctatctaaca atgaaatcaa tgaaattaat
     7621 atagataaag cagtagaaat agcatcagaa gaactaactt ttggttctaa catgagagca
     7681 tctagggagt atagaaaagc tatgagtaaa gtacttttaa aaagagctat aatggaggtt
     7741 atttaatgtt agtaaagatt aatataaatg ggaagaaaaa aagtgttgat atagaaccag
     7801 aagaatatct ggttgatact ttaagaaaag ttggtaattt aagtgtgaaa agaggttgtg
     7861 acacaggctg ttgtggttta tgtacagttt taattgacaa gaaacctaca ttatcctgtg
     7921 caacactgac attgagagct ttagataaag aaataactac aattgaaggt ttagaaaatg
     7981 aagttaaaga atttagtgaa gttttggtaa aagaaggagc tgagcaatgt ggtttctgtt
     8041 ctcctggctt tatacttact gtacttgcaa tgaaggatga gcttgaaaat cctacagatg
     8101 atgaaataaa gcattatctt acaggaaatc tatgtagatg tacagggtat atgggacagt
     8161 taagagctat caaaacttat ctggaggtaa aatagaacat gaatatagtt ggaaaaggaa
     8221 tcaagaaaat agatggaatg agtatcacaa caggaaagcc tgtatataca gatgatttat
     8281 cagcaaaaga tgctttggtg gtaaagatac ttagaagtcc tcacgcttat gcaaaaataa
     8341 aagatataga tacatcaaag gcagaaatgg ttgatggtgt tgaatgtgtt ttaacatata
     8401 aagatgtacc aaaatctcga tttacacttg ctggtcaaac atatccagag tcatctccat
     8461 atgatagatt aattcttgaa gatgttgtaa gatatgtagg tgacgaagta gctatagtgg
     8521 cagctataga tgaaaaaact gcaattaaag caatgaatct tatcaaagta aagtatgaag
     8581 tacttacacc tgttttggat tttgaagagg caataggaaa taaaactatg gtacatgatg
     8641 aagatagaca ttgtaacttt gatataggta tgcaaagaga aaaaaatata gtatcccatc
     8701 atgattatac aaaaggtgat gtagaaaaag tattaagtaa ttgtgatgta gtgattgaag
     8761 aaacttatta tactcaagct caagcacatg caatgatgga gacgtatagg gcatacaatt
     8821 atttagacca tatgggaaga ttggttgtag taagttcaac tcaaatacca tttcatgtaa
     8881 aaagacattt atctcgtgca ctagaaatac cagctagcaa aattagggtt ataaaaccta
     8941 gaattggtgg cggatttggt ggtaaacaaa ctgcatgtgt tgagattttt tctgcaattg
     9001 ttactttaaa aacagggaaa ccagctaaaa taatttatga tagaaaagaa acttttaatt
     9061 gttcaacaag tagacatgca atgagattaa aagttagact aggtgctaca aatgatggca
     9121 taataaaggc aatagatata aaagcacttt ctgatacagg agcatatgga gagcatgctt
     9181 ctacaactat tggtttggtt ggagaaaaaa ctttaccaat gtataataaa gttgaagcaa
     9241 caagatttat tgcagatgtg gtatatacta ataagatgcc agcaggagca cttagagggt
     9301 atggagcatc acaaggatgt tttgctgttg aatcaactat aaatatgctt gctaataaat
     9361 taaaaatgga cccaacagat ttaaggttaa aaaatgtggt aacagaaggt gaaactacat
     9421 ttgcatatgg taaaacttta aatagtgtcg atttaataaa atgtataaat aaaggcaaag
     9481 aacttattgg atggaatgaa aaatatcctt gtaaacatct tgataatgga aaaattagaa
     9541 gtgtgggtat ggcaattact atgcaaggtt ctggtataca aggtcttgat aagtcttcag
     9601 ctgaaataaa attgaatgac tgtggagatt atactcttat gataggttct actgatatgg
     9661 gtacaggaag tgatacgatt ttagctcaaa tggctgctga ggttcttgat acaagcattg
     9721 aaaatataac agttatatca gctgatactg acttagttcc atatgaccca ggttcatatg
     9781 cttcaagtac tacttatgta acaggtatgg cagtagttaa ggcagctcaa gacttaagag
     9841 aaaaaataat ttctgctgga gcaactaaac taggtgttga gaaggatgaa gtagaatttg
     9901 atggtataaa attaactagt aaaaaaggta gcactttatt attcgatata gcagttgatt
     9961 ctacaattgg tccagatact aatcaattaa taggtcttgc ttctcatggt agcccagtat
    10021 ctcctcctcc ttttatagca ggttttgcag aaactgaaat tgacacagaa actggaaaag
    10081 ttgatttagt aaattatgta gctgtagtag attgtggaac ggttataaat aaaaatctag
    10141 ctaagataca agttgaaggt ggaattgttc aagggattgg aatggcatta tttgaagaag
    10201 ttagatacac agaaaaagga agaatggaca caaatacatt tatgcaatac aagattccag
    10261 ctagatgtga tattggaaat gttaaagtag attttgttga aacttatgaa ccaactggac
    10321 catttggtgc taaatctgtt ggagaagttg ttataaatac acctttacca gcaatacaag
    10381 aagctgtgtt taatggaatt ggtgtaagaa taaatacact ccctattaca cctgaaaaag
    10441 tttttatgga aatgaataaa taattattta tgaaaattta gactagtttt acatgttaaa
    10501 caaccagtta tgtcatacct gttgaaagat atttaataag tagttattat aatattatga
    10561 attttactta taaaatacaa aattaaataa ggggatgatg ttttggctgg taatctaaat
    10621 aatatgagag cagtaaataa ttttagagga gataagaaca ttttagaatg tttagtcagc
    10681 tttgagggtc gttcaataag tcagagaaaa gtaagggtat tttttaaaga aaaacaaaat
    10741 caaatagaaa ttgattttgc agaagaggaa atttctaaat tggttgaaaa tgttgtttta
    10801 aatacatcat atcaagaaat gttatatgat gaaatagaga aacaactgga aattgattgt
    10861 ataggtactt ggatgatatt atctaaatta aaagatggta gtagagttca ctgattttaa
    10921 aataaattat tagagcctaa ttttaaggct cttttttgct gtttttaatt aacaatagaa
    10981 aacttatctt tagtttgtat ataattttaa tatcatatat tctatatata taaaattgaa
    11041 tttgtatgta ttatattgta taatgtaaaa tgaattggaa aaatagttcg ataaaataat
    11101 ttgtttttat aaaattactg tttaagttta tagattttgt ataaaataaa gttatgttat
    11161 taattttttt agaaataaaa cttttactag gagacagata tgagaaaaaa tagtattaat
    11221 atagcactaa ctgcagcaat tacagcaatc tatgcagttt taacattatc attgggattt
    11281 ataagttatg gaccaataca atttagggta gcagagatta tgatgctttt ggcattttta
    11341 gataaaggat atatagtagg tcttacatta ggttgttttt tagcaaatgt aataggacca
    11401 tatggtgtac ctgatataat ttttggaaca ttagccacat ttataagtgc aagtatgatt
    11461 tatattacta gaaagttagg taaacagaat aaaagtactt tagttatagc ttctttatgg
    11521 ccaactgtta taaattcgat tgtggttggt ctaatgttaa atgtattctt tgggcttcca
    11581 ttaattctat ctatgataca agtaggtttt ggagaatttg tagtagtgac aattgtagga
    11641 gtaccatttt tctgcttttt aatgaaaaaa tataaaaatg tcattaatat taaattttaa
    11701 atagtgtata gtaatttaat taattgagag tattattagt gaaaagaggt gtttatttgt
    11761 cgaaggtaaa aagattgact gatgtttgga tatatattaa tgataagcaa aagtttacaa
    11821 taaaagagtt gtcagaggaa tttaatcttt cagctaaaac aatacaacga tatttaaaag
    11881 aattaaataa aatgggactt ccaattcaag cagaacaagg tagaaatgga ggttataggg
    11941 tgctaaataa tagctatata cctcctgtta tatttacaga aaaagaagtt atgattatac
    12001 tatttgcact taagtcactt cagatttata attataagtt aatggaaata gaaattaatt
    12061 ctataatgag aaaagtaagt tatgaacgta aaagcagtat aaaagtaggt atagaaaata
    12121 ttaaaagata cattggcttt gtagctaatg atactaatga tggagatttt aagagtagta
    12181 atgtaactga gttttttaga gcttcttcag aatctctaat tttaaatatt aagtataagt
    12241 gtggtaatca gattttggaa aaaaacgtat gccctatagg tatatattta aataagggag
    12301 catggttttc accagcatat gattgtaaaa gtgataaaat aatattagtt catatagata
    12361 atataattga tataaaaaga acaggagaaa gtaaaaaaat acatataaac ttggaagaat
    12421 ggttacatga agtatataac aatttatatg gaaatagttc aactactaaa aaatttggtg
    12481 aaattattta tctaaatgta ttattaacta aagaaggtgt ttctaaaata aaggacagtt
    12541 cttggaattt agatatggag ctaaatgaag atggtagtgg aataattagt acattcataa
    12601 aggttgaaga tatagattgt tatgcttcga ttttattttt tattggaaat aatgctaagg
    12661 ttatagagcc aaaatttttg aataagctac tttatgataa ggccaatgag ttaagatatt
    12721 tttatgaaga aaatatggta taatatttag aaatgttgaa attaaataat tattaaggag
    12781 atatacgtta taatggaaaa ttttaataag aatgctaaaa taattggtat tggagctgaa
    12841 ggtattaata taataaatga agtagaagaa aaaataaaag caaatatgga tatagaaaaa
    12901 ataaacataa atcaagagat tgaaaaagaa tatgttagaa gtcttttaga tggtgtagat
    12961 atattatttt tgatatattc atctgaagat aaacatatta gagatattat aaaagctgtt
    13021 tcatatatgt caaacgaaag aagagttcta tctataggaa tggattgttc agagaaagaa
    13081 aataaagaag atttagagct tgggagagag tttaaaatca ataatgatag tatatttaaa
    13141 tttgttgatt taatgaatat catggtagag tctatttctg attcttgcat gataaatata
    13201 gatattactg atttaaaaga agctattgta ggagataaag gaattaagta ttcttttgaa
    13261 gagtttgaag atacaaagtc atatagcgag atagctgaca ttttatttga caggatggaa
    13321 tatattggtg aggaatttat aagtaagaaa ggaattgtat ttgttgaagg aagtccagaa
    13381 ttttctataa tggaattgaa tgatttaata agtaatattc agagcaaggt agaagaaagc
    13441 tatgaagtaa tattttcttt atatataaag gaaaatttaa atggaaatat tagagtaggc
    13501 ttattgtata attagtatat tttagatatt aaggagagtg tatatgtctg aaaatataag
    13561 aggtacagag tattggttaa tggaagaatt tttttatcag gaaacagaca caccacagat
    13621 ttttagagaa gaaagcataa ttgcacttgg atttgacata aaaataaatt taaaaaaagt
    13681 attcagtatg gggaaaaaat cagaaaagta tattaatgag aattgtaatg ataaagattt
    13741 tataaaaaag tttatgtcga ttaaaaaagg agattatttt ttatataaag catatcaaaa
    13801 tatagaagaa aaagttaaat gtaatgtgtc tattggcata gttgaagaag attttgaaaa
    13861 tggatatgaa ttaagtcata ttttagggca tacactacca gtaaaatgga tgcataattt
    13921 tgatacagtt tcaaattgta aagtatatac aagtgaattt tataaattaa ataataatga
    13981 tattagctca tatttagaaa ttattaagtc acaagataag aaattattga gaaaaagaat
    14041 agaagaaagt gaaaataata aatggtattt aaaaaactat tattataaaa atgtagataa
    14101 gtgtgtagat gatgaagaat tatttattca gcccattaat ctagaacaat tagaaagcct
    14161 aaaaaatata aagacagatg attatatttt attgtataat agcgagaaag taatttgtga
    14221 aaatgaccat acaatgaaga gtctatttgg cgtatgtcaa gttatggaag ggttcgataa
    14281 agagaaagta agtgctttgg aaggtggata tttttttcct gttagatgga tagagtatga
    14341 aaaggagaat atagacggaa atgcatgggt tatatttgaa aataatagta tagttgaaaa
    14401 gtatgtaaaa agatttacta ataaaatgcc attaaatact atactttatg gaccaccagg
    14461 aactggtaaa acatacaata caaaacagat ggtttacaat ctagtaagtg aaaccagtct
    14521 aagaaacaaa tactatacag gttacttaga ttataaaaac aatgtagaat tttgtacatt
    14581 tcatcaatca tatggatatg aggaatttat agagggtctt aagtctgatg gtgaaggcaa
    14641 ttttaagcca gaagatggga tattaaagga tatatctata gaagcatgtt ataatgcatt
    14701 gaaatttgaa aaaaagatga gctttgagtt ggaaaaagat agcttgactt tagaaactat
    14761 aaaagacagg aaaaagaaat tagtattaga aaatatagaa tatagtgaga gttttaattt
    14821 tgaagaatca gaaaactatg ttttaataat agatgaaatt aatagaggga atgtatctaa
    14881 aatatttggt gagctaataa cattattgga agatgataaa agacttacta aatcaaatca
    14941 aactattgtc aaactaccat attcaaagga aaaattttct ctacctccaa atttgtatat
    15001 agttggaact atgaacacat cagataaatc aatagcactt ttagatatag ctttaagaag
    15061 aaggtttaat tttgaagaaa ttatgccaga ttatgattta ttgtctattg tagatggtat
    15121 agatattaga aaaatgctat atacaatcaa taaaaggata gagtttttat atgatagaga
    15181 tcatgttatt ggacaagctt atctacttgc tgtagaaaat attaatgata tagtagtagt
    15241 atttagaagt aaaataatac cacttcttca agagtatttt tattatgatt cagaaaaagt
    15301 aggtttgatt ttaggaggaa ttggaagaaa tgaaaaagat aaatacattg tttataaaga
    15361 aaaacttata gcttctgagt tatttaaaaa taataatgcc tcaataatgg agagtaaagt
    15421 taactattgt ataaaacaaa acatatctag tgaggagcta aaaaatatat atgagtaaaa
    15481 atatatatgt taaggaaacc tatgaatgga taagggtagg taatggtgaa aatgagctta
    15541 cagaaattga atatgaaaaa ttgcttaaat atctagagaa caataatgat gtattaaaaa
    15601 gtaatataat agatattaaa tacaaaaaac ttagatttat aaactatgta ggcatcatat
    15661 gttttgaaaa tgtaatactt gaaatacttc ctaaattgtc attaagtgat aatcttgtaa
    15721 aagatagaga gatactatta cagatgcttt ctatatgtaa taaaattcca attacaatga
    15781 atgaaaaaat aagattgagt ttgaaaaatt ataatctctt aaattttttt gttatgtatt
    15841 tcattgagag tatgcaaaca cagatgaaaa aaggtattta ttttgaatat ataaataaaa
    15901 tagagaactt aaatgttatg aggggtaaaa tattattaag tacatatgca aaagaaaaag
    15961 gcatttctcc aatgaaaata agatgtgaat atgatgagta tagtgaaaat aatttcttga
    16021 atcaagttct taaaaaagca tgtatatcta ttttatgtag aataaatgac aattcaattc
    16081 aaggcaagat taaaaaaatt ttaagctatt ttcaaaatgt agatttaatt tatatagata
    16141 gaaaaaaact attggattat aaattttata aaaacaatga tagatttaaa gattgttatt
    16201 tactagctag acttctactt ttaaatttgt ctatggataa tagtcaaaat aatcaagagg
    16261 cattttctat attatttgaa ataaatactt tgtatgaaga atatatagga attttaatca
    16321 aaagtatatg ggacaattca tttagagaaa catatataca agataagagt aaatttcttt
    16381 tgaaaaatga acaaacaggt aagaaaaact ttaatttaag accggatata gttttatatg
    16441 acttaaaaaa tgaatatgaa attataattg acactaaatg gaaagctatt gaagtagatt
    16501 caaatgtttt ttataggtca agtgatatat accagatgta tgcatatata acagcatatg
    16561 aaaatgcaaa aagatgtata ttattatatc cttgtattca aaaagataag aattatagtt
    16621 cctggaaatt atcagaatca tttaagggta agtttataga
//
LOCUS       DAEMIK010000092        16561 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEMIK010000092 DAEMIK010000000
VERSION     DAEMIK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.092"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1214
                     /locus_tag="KPK87_002481"
     CDS             <1..1214
                     /locus_tag="KPK87_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2538624.1"
                     /translation="YYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTN
                     KYEICARMDNDHFILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKI
                     TKKDDIKKIDLLIDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEK
                     GEFIVYYQPKYSLDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFV
                     FEEVCKTLNKWINKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELT
                     ESVVFDNLNILIDIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIET
                     TDTKRGKIIISKIVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLD
                     EFEKHLNFKFD"
     gene            1361..2107
                     /locus_tag="KPK87_002482"
     CDS             1361..2107
                     /locus_tag="KPK87_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538625.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            2107..3987
                     /locus_tag="KPK87_002483"
     CDS             2107..3987
                     /locus_tag="KPK87_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538626.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGAVCLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGILI
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKTNFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            4106..5497
                     /locus_tag="KPK87_002484"
     CDS             4106..5497
                     /locus_tag="KPK87_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2538627.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            5604..6644
                     /locus_tag="KPK87_002485"
     CDS             5604..6644
                     /locus_tag="KPK87_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2538628.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            7133..7786
                     /locus_tag="KPK87_002486"
     CDS             7133..7786
                     /locus_tag="KPK87_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2538629.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            7862..8071
                     /locus_tag="KPK87_002487"
     CDS             7862..8071
                     /locus_tag="KPK87_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538630.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(8211..8777)
                     /locus_tag="KPK87_002488"
     CDS             complement(8211..8777)
                     /locus_tag="KPK87_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG2538631.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDIIKK"
     gene            8938..9591
                     /locus_tag="KPK87_002489"
     CDS             8938..9591
                     /locus_tag="KPK87_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG2538632.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(9749..9925)
                     /locus_tag="KPK87_002490"
     CDS             complement(9749..9925)
                     /locus_tag="KPK87_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538633.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
     gene            10453..11397
                     /locus_tag="KPK87_002491"
     CDS             10453..11397
                     /locus_tag="KPK87_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG2538634.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            11419..12045
                     /locus_tag="KPK87_002492"
     CDS             11419..12045
                     /locus_tag="KPK87_002492"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG2538635.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            12079..13128
                     /gene="hisC"
                     /locus_tag="KPK87_002493"
     CDS             12079..13128
                     /gene="hisC"
                     /locus_tag="KPK87_002493"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG2538636.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            13131..13709
                     /gene="hisB"
                     /locus_tag="KPK87_002494"
     CDS             13131..13709
                     /gene="hisB"
                     /locus_tag="KPK87_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG2538637.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            13711..14316
                     /gene="hisH"
                     /locus_tag="KPK87_002495"
     CDS             13711..14316
                     /gene="hisH"
                     /locus_tag="KPK87_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG2538638.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            14313..15035
                     /gene="hisA"
                     /locus_tag="KPK87_002496"
     CDS             14313..15035
                     /gene="hisA"
                     /locus_tag="KPK87_002496"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG2538639.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            15017..15775
                     /gene="hisF"
                     /locus_tag="KPK87_002497"
     CDS             15017..15775
                     /gene="hisF"
                     /locus_tag="KPK87_002497"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG2538640.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            15803..16471
                     /locus_tag="KPK87_002498"
     CDS             15803..16471
                     /locus_tag="KPK87_002498"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG2538641.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVNKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         6578 a         1546 c         2869 g         5568 t
ORIGIN      
        1 attattatca agataatttt tacttagtac aattggatgt caataaattt aaatatataa
       61 atgagatgtt tggatatgct gaagggaata agatattaat tcatatatca caggttttaa
      121 ataataatac aaataaatat gaaatttgtg ctaggatgga taatgaccat tttatattgt
      181 tgattgcttg taacactgaa gatgaacttt taaatagatt atctaaaata aataaagaaa
      241 tttgtaactt aaatactact aactcatcta agtataaaat tgttatgtca agtggaatat
      301 ataagattac taaaaaggat gatattaaaa agattgactt gttaattgat agagcaaata
      361 ttgcagcaaa aagtaaaaag gaaaaatatg aacattcata ttcatttttt aatgaagata
      421 caagaaatcg attgtacaag gaaaaacgat tggaagataa tatgaataaa gctttagaaa
      481 aaggtgaatt tatagtatat tatcaaccta agtatagttt agatgatgta aatgaaattg
      541 aaggagcaga agccttgata agatggaata gtccagaatt tgggtttatt tcacctatag
      601 attttgtacc tttatttgaa aagaatggtt ttattgtaaa tattgatatg ttcgtgtttg
      661 aagaagtatg caaaacatta aacaagtgga taaataaagg gtatactcct gtaccaatct
      721 ctgtaaatat gtctagagtt catttatata gggataattt tatagaaaat ataactgatt
      781 tgataagtaa atacaatata tctcctgagt ttattgagct tgaactaaca gaaagtgttg
      841 tgtttgataa tttaaatata ttgattgata taatgaaaaa gattaaagag ataggattcc
      901 ttatatcaat ggatgatttt ggttcgggat attcatcttt aaatctgtta aaagaccttt
      961 cttttgatat tttaaaatta gatagaggat ttttaatcga aacaacagat acaaaacgtg
     1021 gaaaaattat tatttctaag attgtggaaa tggcaaaagc gattgatatt aaagtaatct
     1081 gtgagggcgt agaaacttat gaacaagtag agttcttgaa agagataggt tgtgataagg
     1141 ttcaaggata cttatttgct aaacctatgg tattagatga atttgaaaag cacttaaact
     1201 ttaaatttga ttaatcatta ataattatta gactataaga taccctaaca acttacttac
     1261 aaatatgtaa gttaattgtt agggtatttt tatggttaaa gtagtatatt cacgatatta
     1321 tggtattgta gtaaaaataa aaaattatgg agaatataaa atgaacatat tgagtattag
     1381 aaatatatca aaaacatatt gtggaaatat tccatttaaa gcattagata aggttagtct
     1441 aaatatagaa aagggagaat ttgtgtctgt tatgggacca tctggaagtg gtaaatctac
     1501 tcttttaaat ataatatcta cagttgatag acagagtgaa ggtgaggttg tattagatgg
     1561 atatgatgta agtaaattga aaggagaaaa acttgctgaa tttagaagga aacaacttgg
     1621 ttttgtattt caggatttta atttgattga cactttaact gttggcgaaa atattatgtt
     1681 gccacttaca ctagaggggg caagtatcaa agatatgaat atacaaacta aaagtatatc
     1741 taaattttta ggaatagata aaattttaga tagaaaaacc tatgaaattt caggaggtca
     1801 agctcaaagg tgtgctattg ctagagctat tataaataaa cctgccatac ttcttgcaga
     1861 tgagccaact ggaaatcttg attcaaaatc tactgatgat gttttgaaat tatttactag
     1921 gataaacaaa gaacaaaagg taacaacact tatggtaact catgaagcat atagtgcaag
     1981 ttattcagat agagtaattt ttataaaaga tggttgtata tatacagaaa taaaaaaatc
     2041 tgaaagcagt aattctttct attctgatat actagctgtt ttgtctcaaa taggaggtgt
     2101 aagatagtgg attttagaaa gtttgcttat aataatatag ttagaaattt cagggcatat
     2161 ttagcatatt ttttaagttc tgttttttct ataatgatat tttttgtttt tgcagtgtct
     2221 atgttccatc caattataac aaaatctgga atgcagagtg gttctactgc atttatggct
     2281 cttatgactt cagaattaat aatattaggt tttagtctac tttttatatt gtactcttta
     2341 ggaaatttct taaaatatag attgaaagat tttggagtat tgatgataat tggaatgagt
     2401 aacagacaac tgaaaaaatt gatattaatt gaaaatttga taataggatt tttagcagtg
     2461 atgttaggaa tattacttgg tttatcaatt tcaaaattgt ttttattgta tttaagcaaa
     2521 ctgttttata tgaatttaac tgaattttat tttccagtaa aagcaatgct attgactatt
     2581 gtatcattta tgattttatt tttgataaca ggaccaatga gcttaaaatt attgagtaaa
     2641 aagagcattt tagagctttt agtaggtaca aaaaagccta aaaaagagat aaaatcttca
     2701 aagctatttg gaataattgg tgcagtatgt cttatattag gatatgctat gatactattt
     2761 ggcaataagt taaaaataaa tggaggtatg tatacaacag cactattaat tactttgggg
     2821 atatttttat ttttttctca atttactatt ttgatattaa agtatttaaa aaataaagaa
     2881 aaattttata agaacaaagt aaatatgtta cttataagta atttgatata taaattaaag
     2941 gataatacac gattgttatt tttaataaca atacttttat cggctacttt agtagcattt
     3001 acaacaactt caacacttgt aagttctcaa ggagaagctt ctaaaaataa tttcccaatg
     3061 gtttactcat atttttcaga gcataataat gaaaaagaat atgaagaact acaacaaatt
     3121 agagaagcaa tcaaaggata tgattataag gagttaagtt ttcctgtact taaatataat
     3181 agggaagtct tactaagtgt aaagaattat aacaaattat caaaggaatt gggattagat
     3241 aaaattagtt caaagttaga aaaatatgaa gggattctaa ttcctagtag caattcacaa
     3301 gaacatataa attctttaaa acaattaaaa aattatagta taaaaaattt aaatatagaa
     3361 gtaaaaaaat caaaaaataa aataattttt cctacaggac tgtttagtaa agttgtggtt
     3421 ataagtgatg acttatacaa tgaagtaaaa ggtgaatttt caaaaactaa ttttcatgga
     3481 tttgattata aaaactggca aagcagtgca aagataactg aaaatttaaa agaaaaacat
     3541 tttaatttgg atagagaata taacaggtca tattttttaa cattaccaga tatgtattta
     3601 gtagagttac aacaaagtaa aattcttcaa tttatgggtg tttttatagg agtgatatta
     3661 tttatagcag tttttagttt tttatacttc agattatata cagacgagat agaagataat
     3721 gtaaagtaca aaaaactatc aaaaataggt ctatctttta aaaatatgaa taagattgtt
     3781 agtatagaga taggtacatt gttttttata ccgtatttta tagcaattat aaatagcata
     3841 atttctttac tgtttttaaa tcaagtatta aataatagtt ttaagttaga aaatttgggt
     3901 atattactac tgatatcatc tatatattca ctttattttt acttattaaa aaaaatttat
     3961 actagaaaag tttggttttt tgattaaagt ggtatataat agatatataa attaatattt
     4021 aaaaagtagt taatattagg ggagggcatt tgcacctccc ttgtaatatg tggaaaattg
     4081 aatattagaa ggaggaggag acttgatgga aaatcaacaa ttattaggaa cagaacgaat
     4141 tagtaaatta ctactaaaat attctatacc agctataata ggtatgttgg taaatagttt
     4201 atataatgta gtagatagaa tttttattgg aaatatacca ggggttggac cacttgcaat
     4261 aacaggtctt ggtgtaacaa tgccaataat gacaataata cttgcatttg ggatgttgat
     4321 aggtatagga actactacta ccatatcaat aaaacttggg caaggtaaag tagaagaggc
     4381 aagaaaactt ataggaaatg caatgacgtt atctgtaata acaggaatta tcataatgat
     4441 acttggaata ttatttgcaa ataaaatact tacattgttt ggagcaagtg aaaatacttt
     4501 aatatatgca aaatcatata tcaatattat attattaggt acagtagtaa atcttctttc
     4561 cttttcttta aaccactcaa taagagctga tggtagtcct aaaatttcag caggaataat
     4621 gatagtagga tgtttaacta atatagtttt agactggata ttaatatttg gattcaattt
     4681 gggaatacaa ggagctgcaa ttgctacagt aacttctcag gctcttacag ctattttaac
     4741 tatagggtat tatataagtg gaaaatcaaa cttacgattc agtaaatcta atctaaaatt
     4801 agataaaaag ctaataaaag cagtgtttgc aataggtatg tcgccatttg ctatgcaact
     4861 tgcagcaagt ttagttcaag taatatcaaa tattgcactt aaaactcatg gtggagattt
     4921 agctataggg gctatggcta ctatttcttc tattgcaatg gtatttttga tgcctatctt
     4981 tggaataaat caaggagcgc aacctattat aggtttcaat tatggagctg aaaaatatga
     5041 tagggttaaa aaagcatatt taggttcatt agtagtagct actataattt tatgtatggg
     5101 tatggtagta gttatgcttt tcccagaagc tattataggt atatttaata aagaccctga
     5161 acttatgaat atatctgtta atggattaag aatatactta cttatgttgc caattgtagg
     5221 cttatcagtt acaggaacaa actttataca atctatagga aaagcaaaaa tggctatgtt
     5281 attgagcctt ttaagacaag taattttatt gataccagca gtattaattc ttcctacatt
     5341 cttaggctta cagggtgtat ggacagctca accagtgtct gattttatag ctacagtaat
     5401 tacaggtata gttgtattta gagaattaaa aagatatact cctaaaactg aaaaattaaa
     5461 tgaaaatgaa agactaaacg agataactac tgagtaaaaa tataaattca gataattgag
     5521 caagaaattg caaaaaatac gattatatga taaaataaaa ccgctgtcaa ggataagaga
     5581 aattctaatt atggagtgat tttatggaat ttacaaagaa aagcaaatta aaaaatatgt
     5641 atgtaaacat gaatgtagct agaatgttgt ctaagataaa tgagtataag ggtagacaac
     5701 tgctttacaa aaaacaacca aaagaaattt tagaaaactt agaaaaaaaa tctttagtag
     5761 attgttctga atctacatac attggaaatc aaagagatag tagtaatttt aatctagaaa
     5821 agttgataag taatgaagtt acacctagaa gtagagagga attgagtatt gttgaataca
     5881 gagatgtggt aaaaactatt aacagtgctt atgagagtat tcccatcagt tcacaaacga
     5941 ttcttgagct tcatggatac ttatataaat tttcatctac tagaggagga agttataagt
     6001 cagataatga ttttattgaa cacaatttaa aacctagcga atttataagt actgattcaa
     6061 gtgtcaataa agttgaaaaa gctgtagaag aaatatgtga agcttataat acattgatag
     6121 aggaagatga aattgatata ttaatattaa tttctgcatt tgtgttagat ttcatattga
     6181 tacatccatt caaagaaggt aatataaaaa tggcgagagt acttattcta ttgttactaa
     6241 ataaaaatgg gtatgaagtt ggaagatata taagtttagg aaagatattt gatgatagtt
     6301 catatgaata ttatagtaat ttaaattatt tgaaagcctc aataggtagt gaaaaagcag
     6361 atatgaatgc atggattgag tattttttag agacgatatt aactgcatat gagaagctgg
     6421 atgatagttt aaatatatct gataagaaaa gacaaacaaa aactagtaga atagagaaaa
     6481 taataaattc tacccttgga tattttacaa aagaagatat aagagattta tgtcctgaca
     6541 ttccagaacc tactataaat agagttttta ataatctaag aaaacaagat aagatagaag
     6601 ttgttgcaag aggaagaagt gcaaaatgga agaaaaaata ttaaaaaatg tttagataac
     6661 taaaatggta tagtgtttta cactatacca tttttaatgt cattagcatg agcaataatt
     6721 taacaactta agcaatatca attttattta ttgattattt ttgaactgaa aaatggcgtt
     6781 atatggtata cctcagtggt aattaatttt ggatgagaag atataatatt acagaatata
     6841 ttaggagtat atgtagaaaa aatataataa aagtgtgagg cttagtaatt aacaaattat
     6901 gtggcttcac acttttttat aaaataaaga tacttagcag aataatattt tagttaatat
     6961 atttataaga tggtacttgt ttgggtaaaa gtataaatag taaaaaagta gaagtaatgt
     7021 gtataaaaac ataagttaca ttctgtaata atagttattt tataattcaa caaaagaata
     7081 gaatgggtat atttgtgaaa taataatata aaataatctt aggagtgagc ttatgaagaa
     7141 aaaattatta tatataaatg taaattcaaa acctgaagac ttatcttcaa gtaaaactgt
     7201 agcaagaaaa tttataaata aatttatgga aaaaaataaa gattttgagg ttgaagagat
     7261 agacctttat aaagaacata taccaagact tgagtatcag tactttgaaa aaagaaatag
     7321 catagttagt gaagaaaatg ctaaaaattt agacgataag gatagaaaag agcttacaaa
     7381 gattagaaat ctttgtgacc aatttgtgag tgcaagtgta tatgtaatag cagcacctat
     7441 gtggagttta tcttttccag caccacttaa agagtatata gactgtataa ttcaagatgg
     7501 aaaaactata tcttttgaag gcaatgataa accacaagga attttaaatg atattgatag
     7561 aagtatggta tatatacaat cttctggtgg acacatacca tgggtgttga agccagttat
     7621 gaataaaggg ttaaattatg tggaaagtat tatgaaattt ataggaatta aaaaatttga
     7681 tgaattgtta gtagatggaa ctggaactag tgaagaagaa agacaagctg caatagaaaa
     7741 agcatctgaa aaaatagatg gtataataga tgggatgaaa ttttaattat aatattttat
     7801 taaaaatata aaggaaaaac ctcttttaat gtagaaactt atattgaagg aggcgagaat
     7861 catgcgagaa gaaaaaagta atgaaaagta tgattgttat tggtgtaatc aagagaataa
     7921 tttctgtgta gaaataaaag ataatatagt catgatagat gatggcactg gtacgttaaa
     7981 acaagccgtt ttcatagggt ataaacaaat ccaaattaat ttaaactgtt cacattgtca
     8041 aaacttaaat agaataaaat taaatttgta gaattttaaa atggaaatta ttgaaggaat
     8101 taaaaataga aagtgttaat atttagactg aaaattatat tttatacaat aaaagtttat
     8161 ttaaataaca ttgtatttta atatttattt tcagtcttac tttattaaaa ttattttttg
     8221 attatgtctg gaaaatatct gtctactaat ttttctgaaa tattttttaa ttggtcagcc
     8281 attgaagtca gacttaataa ggctatcaat aaatcatctt tattttctgg atttatacta
     8341 atattttcaa aattagactc tataaagtcc ggtatattct ttctttgatt ttcttttatt
     8401 aacatgaatt tttcgtaaca tgaaactaaa tctttttcac tgaaattatc cttttcatac
     8461 acaccatgta agattctttt aatttcttgt attgtaagtg tatttttcat agaataaata
     8521 attatcatct gtaaaatttg ttctttagta tacttttttc ccttaactgg tttgattaat
     8581 ccctctttgc tgtaattatt tatcatggtt ttagtaagaa ttttatcact ttcaaatctt
     8641 ttattagcag aaagcttatt gtcaattaga gaaataattt gatccatata taagtcaata
     8701 gatggaatat cgtttgaagt tatatctgca ttatttagtg tttctagtat tatagtatta
     8761 agttcattat tcatcatatg ttacctccat aatatatata atacactaaa ttagaaagta
     8821 aaacaatatt aaggcttgta aaaaaaacat aaagtggtat aattaaatat ataatatagt
     8881 aatcaaaact atatttatga atctgatttt taaaatacat aattgtagga ggacgaaatg
     8941 tatcaatttt ttttaaaggg tagagaccca ataagcagtt taactcattt tataggtgct
     9001 tgtttatctc ttttagcaac tataatttta gtttttcaat ctgttacatt acaggaaact
     9061 tctctcttga tgatagtatc tgtatcggta tttggacttt cactaattgc tttatatagt
     9121 gcaagttcgt attatcattt tttaaagggg acaccagaac aagaattgtt ttttagaaaa
     9181 gtagaccatg caatgattta cgtattgatt gctggctctt atacaccaat atgtctgaat
     9241 tttatggaaa aaaaagaagg cataatattt gttacagcaa tatggatagt tgcatttatt
     9301 ggtataataa taaaaatatt ttggatggat gcacctagat ggttatctac cagtatatat
     9361 ttacttatgg gatgggcaat tgtttttgat ataaatgcat tcaattcaat tcctaaggat
     9421 tgccttagac tattaattat ggaaggtgta tcttactcta taggtgcaat tatttatata
     9481 attaaaaagc ctaatataag tcctgagttt ggatttcatg aaatattcca tatttttatt
     9541 atgataggtt ctttatttca ctttttagca gttttattgt atgttttata gttttaaaag
     9601 atttgattaa ttgatactaa aattctacaa tgaataaaat ggaactgcct tatatagatt
     9661 atatcttcga caataagaca gttccttatt ataaatatta aattctactt ataataaaat
     9721 tttgataaaa atagtccatt caaaatgctt attccataat tcctttaatg tacttttcat
     9781 ttacgacata tagtttgaat ttatgtacta taattatatc ctctaaattc tctgaaacac
     9841 taacaattct gacacatttg tttctaattt ctacaggtaa atctaaatca tttgttaaat
     9901 tcacatagtc atcatattta tacataaaat catcccttta cattaagata tacttattat
     9961 actatatttt aataagtttt tttaaattat tacaattttt cccgaaattt cattttttat
    10021 acataaaatt tccaaattag gtaaagatta aattttttat tgtataaaca aaagttacat
    10081 ataaaatatt ttattcaatt ttttaaataa ctattgccaa tttttgaaaa atatattatt
    10141 attaatagta acaaaaaact gtaaatttag aatattaata aaaattattt gggaggaaat
    10201 tatcatggtg agattaaata tagtaaaatt aaatacaaaa ttaaatcatc accatcatca
    10261 tagattaaag ggattgtaat atgaaattaa ttcatagaca caagccctgt ttttagtgta
    10321 attggtcttg tatctatgat gggaagtaat tttaagagaa aattatagcc atataggtat
    10381 aaagcctatg tggctttttt attgttttaa atttgcaaat ataagagtaa atttgatgaa
    10441 gagggtgaga atgtgaaatt tttgaggatt gaagatgagg ttatttattc taaaaaaaga
    10501 tataaattaa aaagggaaat agataaactt tttttggatg aagaatattt tcaaatagaa
    10561 ccacaattat ttgaagaata tgatgaattt acaactataa ataataaaat tccagaagaa
    10621 tctatggtaa aggttgtaaa tggaaaagta atggtactta gagctgatat aactacaagt
    10681 ataataaaaa gccttgtacc aagatgggaa gatggtttaa agcttaagct tttttataat
    10741 tcatcaatat ataaaaataa aaatacagtt ggaataaaag agataagaca aataggttgt
    10801 gagtatctag gagaagcctc tgtagaagca gatagagaag tagtgaaatt agctcttaag
    10861 atacttgaaa aatacaataa taattttatt ctagaggttg ggagtagcaa gtatattcat
    10921 ggtttgttag aagaattaaa tttaaataag aattgtgaaa atcaaattaa aaatttacta
    10981 tatacgaaga acactcatga attaaaagta tatattgaag aattaaaaat taagcgtgaa
    11041 gtaaaagagt tattatcaaa tattttaagt ttacaaggga atttatataa tgttattgaa
    11101 aaatcaaaca agttctattg taataataag atgaagcaag ctcttgaaga attaaagcaa
    11161 gtaaataatt tgattgagga atgtaatttt ttagataagg ctagatttga tttatcaatg
    11221 attactatgc ttgactatta tgaaggaatt atgtttagag gatactaccc aaactcatat
    11281 aaagaaattc ttagtggtgg aagatatgat tctttaacta aagagtatgg aaaagaaatt
    11341 ccagccattg gatttacact aagtgttgat gagttaatga agtatgtata taagtaaagt
    11401 ttagaaggag ggattgcttt ggattatttg actatagcac tagcaaaagg acgaattgaa
    11461 ggggaatcat ttaaaaaatt taaaaaaatg ggtctaggag atagtataga cacagatacg
    11521 agaaaactaa tttttaaaga tgaagaaaat aaaattatat atatacatgt aaagccatca
    11581 gatgtagtta catatgttga aaaaggagtc gctgacctcg gtatagcagg taaagatact
    11641 attttagaga atgagacaga tgtatatgaa atatatgatt taggttttgg aaaatgtaaa
    11701 tttgctgtag caggattaaa gggagatagc atatatagag aagatgaata tttaaaagtg
    11761 gcaacaaagt atcccaatat agcaaaaaag tattttaaag agaaaggtca aaaaattgaa
    11821 ataataaaat taaatggttc tgtagaacta gctccaatag tagggttgtc agatgtaatt
    11881 gtagatatag ttgagactgg caatacactt aaagcaaatg gtcttgaaat attagaagac
    11941 atatgtaata taagtgctag aattatatca aatagagcta gctatagatt taaatatgaa
    12001 caaattcaga atataattcg tttatttgaa gaacttgata attaaaatat aattgagctt
    12061 tataaggagg gatttaagtt gagagaaaaa gaaagcataa gagagttaag gggatatgaa
    12121 ccaaatcacg taaactgtaa agtaaaactt gatgcaaatg aaggaagtaa gaggttgttt
    12181 aaatacctta ttaaagaaat ttcagatagt gatatagatt taaatttata tcctgaagat
    12241 tcttatagtg atttgaaaga atctataata gattacataa atatttctgg agtaaataag
    12301 aaaaatcttc tagtaggaaa cggttcaagt gaaataattg atttaataat acatactttt
    12361 gtagataaag atgaagtaat attgagtttc tctccaagtt ttagtatgta ttctatatat
    12421 agtcagataa atgggtctaa atttataggt gttgaaagtg atgaaaattt agttataaat
    12481 atagataatg tgattgaaaa agtaaaggag aataatccta aaatagtaat tgtatgtaac
    12541 cctaataatc caactggaac aatattaaag agggaagaga taataaagct tttagattca
    12601 actaatagtc tagtggtttt agatgaagca tatatggatt ttggagaaga aagcatgtta
    12661 agtgatgttt ttaagtatga taatctaata gttcttagaa ctttatcaaa ggcatttgga
    12721 ttggctggaa taagaacagg atatatgtta tctaatagta gtttaataaa ttctgttgag
    12781 aaagtaagac ctccatataa tttaaattca ttatcagact ttattgctac aagagcactt
    12841 agaaataaag atgtggtaaa ggcttatata aaggaagtaa aagaggaaag agaagtttta
    12901 tatgaagaaa tgattggtat gggaattaaa gcatacaaat cacaagcaaa ttttatatta
    12961 ttttattctg aaatcgaaaa tttatcacaa aaacttattg atagaggagt tttaataaga
    13021 aaatttggtg gaaagttaga aaattattac agagttacaa ttggtgataa agaagaaaat
    13081 tctatgtttg ttggagctat aagagatatt ttgaaaaagg agaaataggt atgagaattt
    13141 ggaaggtaga aagaaatact cttgaaacac agattttagt agaattaaat attgatggtt
    13201 ctggtaaagc tgaaatagat actggaatag gatttttaga ccatatgctt accttaatgt
    13261 catttcatgg caagttcgat ttaaaagtga tttgtaaggg tgatacatat gtagatgacc
    13321 accatagtgt agaagatata ggtatagcta taggtgaagc atttaaaaat gctctaggtg
    13381 acaaaaaagg aatacgaaga tattctaata tctatatacc tatggatgaa tctctatcaa
    13441 tggtagcaat agatataagc aatagacctt atcttgtatt taatgctaaa tttgataccc
    13501 aaatgattgg tagtatgagt actcaatgct ttaaagaatt ttttagagct tttgtaaatg
    13561 aatcgagagt aacattacat atcaatctct tatatgggga aaatgaccat cataagatag
    13621 aaagtatttt taaagcattt gcaagagctt taaaagaggg tagtgaaata gtttctaatg
    13681 agatagcatc ttcaaagggg gttttgtaga atgaatataa tagtagacta tggtcttgga
    13741 aatatagatt ctgtgtctag gggttttaga aaagctggaa tagaaactaa aatttctagt
    13801 gatatagatg aaataaaaca ggcagattca cttatacttc caggtgttgg agcttttaga
    13861 gattctataa gtgccttaga taaactagga ttgataccaa taataaagga gcatgtttct
    13921 aagggtaaat ttatgatagg tatatgtcta ggaatgcaat tactttatga aaaaagttat
    13981 gagtatggag aatatgaagg attaggactt ataaaaggaa gtattgataa actggatata
    14041 agtttaaaag tgccacatat gggatggaat aatctaaaat ttaataaggc aaatgatgat
    14101 atacttaaat atataaatga agatgattat gtatacttcg ttcactcata ttatgcaaat
    14161 tcatcaaatg aagaactaat tgcattttca gaatatgaaa agaaaatccc tgctattgtt
    14221 agaaaaggca atgtatatgg tatacaattt catcctgaaa aaagtggaga agtagggtta
    14281 aatatattaa gagcatatgg ggagatgata aaatgataat atttccagca atagatataa
    14341 aagataataa gtgtgtaaga ctcacacaag gagaatttga caaagtaaat gtatattatg
    14401 ataatccatt agaagtagca tataaatgga aaaatgaagg agcagaatat atacatatag
    14461 tagatttaaa tggtgctaga agtgaatttg gtgtaaatac aaaaataatt gaggacatag
    14521 caaacaatat agatatacct attcaagttg gcggtggagt tagggataag gaaaaagtca
    14581 aaagtttaat aaatgctggt gttactagag taatattagg cagtatagcg attgaaaatt
    14641 tgaatttagt tgaagaattg gtcaatgaat ataaagagaa aattgtagtt tctatagatg
    14701 ctaaagatgg aaaagtagca gttaggggat gggaagttgt aagcaatgta gactcattaa
    14761 cattatgtaa acaacttgaa aagataggtg tacaaactat tgtttataca gatatatcaa
    14821 aagatgggat gttgcaaggt ccaaattttg acatatatga aagaatagca aaggaaacat
    14881 cactaaatgt tattgcttct ggaggtgtaa cttctataga agacgtaaaa agactaaaag
    14941 ctatgaactt atatggtgca ataataggta aagcgcttta tgataaaaag atagacttta
    15001 aggaggcaca acaattatgc ttactaggag aataatacct tgtctagatg tgagaaatgg
    15061 aagagttgta aaggggaaaa aatttaaaga tatagtagat gttgatagtc cagaagtact
    15121 tggaaaattt tatagtgatt gtggtgcaga tgaacttgta ttctatgata taactgcatc
    15181 aaatgaagag agaaagacat ctttagaatt tgtgacaaaa gttgcagaaa atataaatat
    15241 accattttgt gttggtggag gagtaaataa attagaagat ttcactgata ttttaagaaa
    15301 aggtgctgat aaagtttcta taaattcatc agcagtcaaa aatcctgaat taataaggga
    15361 agcttcatta aaatttggag cacaatgtgt tgttttatcg atagatgcaa agaaaaatga
    15421 agagggttca tggagcgtat acgtaaaagg tggaagagaa aaaactaatc tggatgcaat
    15481 tgaatgggct gttaaaggtg tagaacttgg agcaggagaa atcgttgtaa atagtatgga
    15541 tgaagatgga atgaaaaatg gatatgatat agaattatta tcaaagataa cttctttagt
    15601 aaatgtacct gttatagcat ctggaggcgc tggaaaaaag gaagattttt atgaggcggt
    15661 taataaatcc aatgtagatg gaattttagc agcatctgtt tttcattttg gcgagataaa
    15721 aataaacgat ttaaagaaat atttaaaaga catgggtgta gaagtaagat tataagttaa
    15781 atactaaaag gagattagaa atatggataa taagtgtaac aatgtataca gtgatgaagt
    15841 agagaaattt ataagaagta taaagtttga tgataaaggg ttagtccctg ttgttgttca
    15901 agaggtagtt aacaaagatg tactgatgtt agcatatatg aataaagatg ctataaagaa
    15961 aaccttaaaa gataaagtag cttgctattt tagtagaagt agacaagaac tttgggttaa
    16021 aggtgagaca tctggtaata ctcaaaaagt agttaagatg tcttatgatt gtgatgtaga
    16081 tactatacta ctttttgttg aacaaactgg tgtagcttgt catacaggca attattcttg
    16141 tttttacaga gacttgtttg atgatagttc aaaaatggag cttgaagtac aaacaaatat
    16201 attgaaagaa ttatatgatt taatcaatga aagaaaaaat aatccagttg aaggttctta
    16261 tactaattat ctttttgaaa aaggaataga taagattctt aaaaaagtag gtgaagagag
    16321 tagtgaggta attatagcat caaaaaatac tgataaaagt gaacttattt acgaaattag
    16381 cgatttagta tatcatacat tggttttaat gatagaaaaa ggtgttgaaa ttgatgaaat
    16441 aaaaaaagaa ttacttaaaa gaagaaaata gaataagata gattaatggg taataagaaa
    16501 atataccact tttaataaaa agtggtatat tttttatttt atattttgat ttatagaata
    16561 a
//
LOCUS       DAEMIK010000093        16300 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEMIK010000093 DAEMIK010000000
VERSION     DAEMIK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.093"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            300..1133
                     /locus_tag="KPK87_002499"
     CDS             300..1133
                     /locus_tag="KPK87_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG2538642.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1226..2158
                     /locus_tag="KPK87_002500"
     CDS             1226..2158
                     /locus_tag="KPK87_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2538643.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKVTGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2210..2692
                     /locus_tag="KPK87_002501"
     CDS             2210..2692
                     /locus_tag="KPK87_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2538644.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2704..3573
                     /locus_tag="KPK87_002502"
     CDS             2704..3573
                     /locus_tag="KPK87_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2538645.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFVCIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3616..4371
                     /locus_tag="KPK87_002503"
     CDS             3616..4371
                     /locus_tag="KPK87_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2538646.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIKAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4413..5234
                     /locus_tag="KPK87_002504"
     CDS             4413..5234
                     /locus_tag="KPK87_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBG2538647.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5239..6000
                     /locus_tag="KPK87_002505"
     CDS             5239..6000
                     /locus_tag="KPK87_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG2538648.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6161..6418
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6531..6770
                     /locus_tag="KPK87_002506"
     CDS             6531..6770
                     /locus_tag="KPK87_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2538649.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7074..8069
                     /gene="ilvC"
                     /locus_tag="KPK87_002507"
     CDS             7074..8069
                     /gene="ilvC"
                     /locus_tag="KPK87_002507"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG2538650.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8122..9810
                     /gene="ilvB"
                     /locus_tag="KPK87_002508"
     CDS             8122..9810
                     /gene="ilvB"
                     /locus_tag="KPK87_002508"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG2538651.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10018..10695
                     /locus_tag="KPK87_002509"
     CDS             10018..10695
                     /locus_tag="KPK87_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2538652.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10864..12900
                     /locus_tag="KPK87_002510"
     CDS             10864..12900
                     /locus_tag="KPK87_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG2538653.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINKNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13091..13657)
                     /locus_tag="KPK87_002511"
     CDS             complement(13091..13657)
                     /locus_tag="KPK87_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2538654.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            13945..14880
                     /locus_tag="KPK87_002512"
     CDS             13945..14880
                     /locus_tag="KPK87_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2538655.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRFKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15345..>16300
                     /locus_tag="KPK87_002513"
     CDS             15345..>16300
                     /locus_tag="KPK87_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538656.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFN"
BASE COUNT         6745 a         1598 c         2834 g         5123 t
ORIGIN      
        1 ctatttatta tttgtttaat atttctaatt tataaaaaaa aaattataaa ttttaaaaat
       61 tataaatgac attttaacta gaattaattg taaaaaacac tatataatac tcaattttca
      121 acattctata caattataca gaaattatac agcataataa tcaccgaaat aacaaatttt
      181 aaaaattaag caaaactttt tgtcgaagtt caacgtctat atatatgtag tgtaaatttt
      241 tgggataaaa tactacaaaa atataaatgt aaaaatatag taatattgga ggtcgaacga
      301 tgagaaataa aaagaaaaaa ataaatagaa agaagttgta tttgctttta agtgctattg
      361 tagtgtgtct ggcttttata gtagttggtg ttcgcgtcag ctctttaaat atgaaggaaa
      421 atgaaattag tagaaatgca aaactgtcaa gttctactat tacagatata gtatctaata
      481 catcaataga agttagtaaa gggccaagta aaagttctgg aaaaatagca tacataacta
      541 tagatgatgg tccatctaaa tttactgacc agatgataaa aactttaaat aaatacaatg
      601 ttaaagcaac tttttttatg atagatggga atatgaagga atatccacag caagttaaaa
      661 atataattaa aaatgggaac acagcaggtt ttcatagtgt atcacatgat attcacaagc
      721 tttatgttac tagtacatca gcaaaagagg aatttgatac aaatgatcaa actttttata
      781 agataactgg taaacattca aaagtaataa gaatacctta tggaagcaaa ccatatacac
      841 cacaagcctc ttatcaggct ttagttgatg ctggatataa aatttgggac tgggacttag
      901 atacagaaga ctggagatct aattcttctc aaattgtaca aaatgtaaaa aatcatatta
      961 agaatagaaa aggtgaagat aaagaccaac ttgtagtttt aatgcatgag aaaaaacaga
     1021 gtgcagaagc tttagattca gttttaaaat tcttatcaga tgaaggttat gaatttgcgc
     1081 cagtagatca aaaccaaata ccaaaaaatt attggttacg taatttagag tagttaaaat
     1141 ataagaatat tgattgatat ttttagaaat tgagaataaa aatgagtgag gtgtaggtat
     1201 attgaataaa aaactttata taggaatggt cggaatattg tctttgatga tggttggttg
     1261 taataaaagt ttagcaaaag taaatgatgt ggaaatcaca aaagaacagt acaaaaaaac
     1321 aaaagcagtt ttatctgcta ctaataatta tataaatggg cagtctttag atgaattaga
     1381 aaaaactttg gataaaaaag gtagaaataa attagaaaat gttataatat cgtttatggt
     1441 ggataatgaa cttttatatc aagaggctaa ggataaagga ctaactccaa gtaaaagtga
     1501 agtagactca aaatatcaag aactagaaga taagatgaat ttgaatacaa gctataagga
     1561 aaaaatggac aaagcaggtg ttgataaaga ttatttaaaa caagaaatat ctagagattt
     1621 agcaatagat aaaaataaaa aggcctttga agatagaata aacataagtg ataatgatat
     1681 ggaagcctac tatacaagtc ataaaaaaga ttttaatgta gaagaagtaa gtgcttctca
     1741 gatacttata tcgactttag ataaaaataa aaaagaagta agcaaagata aaaaagaagc
     1801 tttgaagaag aaggcagata atatattgac taaaatcaaa aatggagaaa gttttgaaag
     1861 tttggctaaa aaatattcgg atgataaagt aactggaaaa aatggaggac agctaggata
     1921 ttttacaaag gatgataaaa atgctgagtt tacaaaggaa gtgttcaaat taaagaaaaa
     1981 tgaagtttca aatgtgtttg aaactagtta tggatatcat atagtcaaag ttactgataa
     2041 aagagaaaga caaaaaagtt ttaatgagtg tcagagttta ataagagaat ctattttaaa
     2101 tgaaaagtat attgagcata taaaaaaact gaatgaagat gctaaaatag acagatagaa
     2161 tttctaacag ctcaatccta gcctgtaatc ggagggagat tttacaggaa tgtttaagag
     2221 tttaaagaga acgaaagttg agaaatacat tatcgataat aaagactctt tttatagaat
     2281 agcatatagt tatacgaaga atgaagaaga tgccttagat gtggttcaag aagccatgta
     2341 taaggcacta tactcagtgg aaaatattaa agaagtaaat tatataaaga catggtttta
     2401 taaaatttta gttagaacga gtatagattt tattagaaaa aaccgtaaat ataataatat
     2461 gacagatatt gaccttatag atgagactgg agagtatgat aaatatactg atttggattt
     2521 aagaagagct ttggaagaat tacctattga atacaaatct attataatat taagattttt
     2581 tgaagattta aagattgaag aagtagcaat aatattggat gaaaatgtga atactgttaa
     2641 aactaggtta tatactgctt taaaaaaact aaaattaaaa attgaagagt agggataaaa
     2701 agaatgacaa gcaaagaaag gttaaataag ttaaaagatg agtatcataa aattccaata
     2761 cccaagaaat tagatactat aataaatcat gaaaaaatag aaaacatata ccgagagaaa
     2821 aataaaaaag taaatagatt aagatttaaa gttgcaatag cctttgtatg tatatttaca
     2881 gtattagtaa atataagtcc tgtatttgca gacaattttt ctaagatacc tgtaattgga
     2941 gcaatagtag aagtaataac tataaaaaat tatagtttaa aaagtgaaaa ttatgaagct
     3001 gagattgata ttcctaaaat acgaggttta aaagataaaa acttagaaca aagattaaac
     3061 agtagtttta tggaagatgg aaaaaggctt tatcatcaat tccaagagag aatggagaag
     3121 atacaatcat caaagaataa aggatataaa tctttaagtt tgagttatag tgtaaaaaat
     3181 aattctaaaa aatttctatc aatagagatg acaaaaaatg agatagaggc ttcgtcatat
     3241 gtgagtaaag tacattatac tattgataaa aaaagacaaa tagttttgac tttgccaatg
     3301 ctttttaaag atgataagta tataaaagtt ataagtgata atataaaaga acaaatgaga
     3361 gaacaaatga aaaaggatag tacaaaatcc tattttatag atcaaaaaaa agacttgcct
     3421 gttgaagatt ttaaaacaat taataagtat caggattttt attttaataa aaatgaagac
     3481 cttgtaattt gttttgatga atatgaagta gcacctggtt atatgggcgc agttgagttt
     3541 gtaataccat ataaagtaat aaaagatttg taaattgaat agtagtttat atataaatct
     3601 tttaaggagg acaatatgac tacatataaa aaacttaaaa aatctatatt taaaattgct
     3661 atattttcaa tatgcttatt agtttcatta gtagatacaa accctatatt tgcaggtagt
     3721 tcctttaatg aaacttcaaa tagtgatacg ataaaggtgt taaatgctaa gagtatcaac
     3781 tttaaacaag acaattatga agtgaatgtg agtattccta aaataaaagg tttaaaagat
     3841 aaaactttag agaataaatt aaacaatgag cttttgcaag atgggaaaaa aatttacagt
     3901 gaatttcaaa ataaaatgaa aaaggaaaat aataagggtc atagatacct aaatgtaatc
     3961 cataatgtca aaaataattc taaggacttt ttatcaatag aaatgacaaa agaagagata
     4021 gaagcttcct catctataac taagaagcat tacactgttg ataaaaatag aggaatacta
     4081 ttaaatttac caatattttt taaagatagt agatatatta aagctataag tgataatata
     4141 aaagagcaga tgagaaatca aattaagaaa gatgatacaa aatcttattt tatagaccaa
     4201 gataaagact ccccagtgaa agattttgaa tctataaata agtatcaaga tttttatttt
     4261 aataaaaatg gaaatcttgt gattagtttt gatgaatatg aagtagcacc tggttatatg
     4321 ggagcagtag aatttgtgat accaaataaa gtaataaaaa gactaagata gatatataga
     4381 agatttttag atagactggt aaggagaatt atatgaatat atatgaacaa gcaataagat
     4441 taatagaaaa taatgaggat ttcgcatttg ctacaataac gtctcattca ggttcaacac
     4501 cacgtgaaac tggagcaatg atgattgtga aaaatgattc tactatattt ggctcagtag
     4561 gaggaggtag tgtagaagca gaatgtataa aacatgcaat taatgttata aaaaacagag
     4621 aatcaatgtt gtataaattt actctaaaca aatctgatgt ggcaaaacta ggaatgatat
     4681 gtggaggtac tggagaaata caaatagatt ttatagatag taaattaaaa agtaatatag
     4741 aaaaatttaa taagagacta aaggaaaata caagtaaagc atatatattt ggtgcaggtc
     4801 atatttcaag agatgttgct gtgatactat ctcttttaga atttagaaca gtagtaatag
     4861 atgatagaga agaatttgct aatcatgaaa ggtttccaga atcagaagta gtagttttag
     4921 attcttttga aaacatacca gattttccca ctgacgaaaa tagttatata attattttaa
     4981 caagaggtca tctatatgat tcaagtgctt tagaatgggc tcttaaaaga gaggctggtt
     5041 atataggtat gattggaagt agaacaaaaa ttggattgac ttacgaaaaa ctcatgaaaa
     5101 aaggttttaa gaaggaagaa ttatcaaaag tacatgcacc aataggcata aaattgaatg
     5161 ctcaaacacc agctgaaata gctgtatgta tagctgctga acttataaat tgtagagcca
     5221 ataaggaaaa ataaactaat gaacacaggg agtaaagata ttgatacttt aaaggttaat
     5281 tttagaggga ttgaaataaa agcagtttta gtatataaaa atagaaaaaa tatttctata
     5341 aaaatagatc catttggcaa aataactgta atgagtcctc ctaaaatatc caaaaaaata
     5401 ataaaagata taattattga aaaaggagac tggatactta aaaagtcaga aaaatgtaaa
     5461 ggaagagagg aagtatataa acaaagaatg tttataactg gcgaaaaatt tctttattta
     5521 ggtgaagaat accatctagt tataaaagaa ctgttgagag attctgtcaa aaaaagtaac
     5581 tgtagaataa ctattaacga atatcaaata gttgttcaga caaatgatac aagtacagat
     5641 tttattaaaa aatctttaaa atcttggtat aagatggaaa gtgaaagaat agtattagag
     5701 agaatagatt ttcttaaatt aaattgtgaa ataatgaagc aattagttcc agcgagtgtt
     5761 aaggtaaaag aacaaaataa gagatgggga agttgtacag ctcaaaaaaa tatttatata
     5821 aactcaaaaa tatctatggc aagattagat gtaattgatt atgtaataat acatgagttt
     5881 agccatttag tacatatgaa tcactctaaa aaattctatg atttagtaaa atccattatg
     5941 cccgattata aagataaaga aaattggcta aaaataaatg gatataaaat aataatctga
     6001 aaacgattat tccaaaatat aggcataaag tataaaagaa gtattattgt gaaaaaaata
     6061 aataatctga aaattttaaa aaaagtcatt gacattgtaa atgataggga atataatcta
     6121 tattaacaac aaaacagaat taaaatataa aattaaaaaa ttaaaagcaa tgacagagac
     6181 aacaatacta atagtgttat ccacagagaa ccaatgttgc tgagaattgg tgttaacagt
     6241 agtataatga tcactctgga gcttttgtac tgaatatttt tagtaagtac aaacgtatgt
     6301 caacgttaca tggctaaggt taaggtaatt aattacccga cctgaaaaaa gaggggtaga
     6361 tgtatttttt atatctacaa ataaggtggt accgcggaat ataactttcg tccttataag
     6421 taatatttat attgcttata aagatgaaag tttttttatt ttaagcgata taaataaatt
     6481 taatccatat aaaattaata aaaaaataat aaaaataagg agggattttt atggaaaata
     6541 ctgtatatgc taaattaaac caaggaatag attctttttt aagagtggct atgactctaa
     6601 gaagaagaga ggttgatata caatcaataa gtatgactgt agataatatg aataattcag
     6661 gtataaaatt aatagtaaat gaagaaaaga catctttaga ttctgtctta aaccatatga
     6721 aaaaactaca tgatataaga gagataactg ttgaaagaat gagacattaa ttaaatatat
     6781 tttataaaac taaagataaa tatgcttatt ttaaaataaa aaactaaaga taaatattaa
     6841 ataagaaaaa tcttagatta aaatataagt gtcattgaat ttattagttt atcacagtta
     6901 ataaatttat tagtttgttg tagctaataa aaactgaaaa ttctaaaatt ataattgtaa
     6961 gattatataa atttagaatt taaatctgtt gaataatatt aaaacaatga taatgagtat
     7021 gaatttctaa taaaaataaa tatcataaaa ataagaaaaa gtattgggag gaaatgaaaa
     7081 tggcaagaat gtattatgaa aaagatgtgg atttagaggt tttaaaaaat aaaaaggttg
     7141 cagttttagg ttatggaagt caaggacatg cacatgcaca aaatcttaga gataacggtg
     7201 tacatgtaat gataggtctt tatgacggaa gtaaatcagc acaaaaagca aaagaagatg
     7261 gatttgaagt taagagtgta gcagaagcta caaaagaaag tgatttgaca atgatgttaa
     7321 tgcctgatga aaaacaaaag aaagtttatg aagagagtgt aaaggataac ttaaaagaag
     7381 gtcagacatt agcttttgct catggattca atatacatta taatcaagta caaccaccag
     7441 aatttgttga tgttgtaatg gtagctccta aaggacctgg tcacttagtt agaaatgtat
     7501 ttacaaaggg aagtggagtt cctgcactat ttgcagtata tcaagactac actaaaaaag
     7561 ctacagagac agttttagca tatgcaaaag ggataggagc aacaagagca ggtgttttag
     7621 aaactacttt taaagaagaa acagaaacag atttatttgg agaacaatca gtactttgtg
     7681 gtggaataag tgaacttata aaattgggat ataaaacgct tgtagatgca ggttatcaaa
     7741 aagaagtagc atattttgaa tgtttacatg aaatgaaact aatagtcgac cttatatatg
     7801 aaggtggatt tgaaagaatg agatatagta taagtgacac agcagaatat ggagattatg
     7861 tatctggtaa aagggtaata actgatgctg caaaacaagg tatgcaaaat gttttagagg
     7921 atatacaaaa tggaaaattt gcaaaagcgt ggattaaaga aaatgaagaa ggacgtgaaa
     7981 acttcctaaa aactagagaa gaagaatata atacagaaat tgccgaagtt ggtagaaatc
     8041 taagaagtat gatgtcattc ttaaaataaa tatatattaa atggattagt ttgagggaaa
     8101 gagtggtgta tagtagtgcg tatgaatgga gcaaaggtaa ttttagaatg tttaaagaaa
     8161 gagggaattg acacaatatt tggatatcct ggtggagctg tgatacctct ttatgatgct
     8221 ctatacgatt attcagatga ttttaaacat ataaggacat cccatgaaca aggtttagtt
     8281 catgctgcag atgggtacgc aagaagtact aatacagtag gagtttgttt tacaacatct
     8341 ggtccaggag caacaaatgc cataactgga atagcaacag cttttatgga ttcttcacct
     8401 atggttgtaa tttcagggca ggtgccaact agtttattag gaaaagattc ttttcaagaa
     8461 attgacataa ctggagcaac attatctatg acaaaacata attatttagt tagaaacact
     8521 aaagaattag ttcctacaat aaaagaagct tttagagttg caaattcagg tagaaaagga
     8581 cctgtacttg tagatgttcc aaaagattta tttttagcag aaatggattt tagtggtgag
     8641 gactatgatt tatgtcaaat agatgattat atggattata agagtgattt tgatctagat
     8701 gatgaaacta atattaagct tttaaatgaa gcaatagata tcataaaaga atctaaaaag
     8761 cctgtaatat atgcaggtgg aggtgttaaa tcttcagaca gtgaggaaat tcttgaaaaa
     8821 tttgcaacta agatagatac acccgtacta aatacactta tggggcttgg aaatatagat
     8881 agaaagaatg aactatctct tggaatggtt ggaatgcatg gaagtagaga aagtaattta
     8941 gcactttcta attcagattt agtgatagcg ataggtgcga gatttagtga tagagtgata
     9001 agtaaaagtt ctgaatttgc taaaaatgca aaaataatac acatagacat agacccatct
     9061 gaaataagta aaaatataga atctaatgta tctttggttg gagatgtaaa gctagtcctt
     9121 agtttattaa ttgaaagggt tgaaagtaag aataatagca attggaaaga agagataaag
     9181 acatttagaa agagtgaggg tgtacaaaca tatgaatttc atccacaaaa tatacttaaa
     9241 aaaataaatg aaaaatatga aacattaaag aaacccactg tggtagtaac tgatgtcggt
     9301 caacatcaaa tgtggtctgc taaatactgg aattttaaag gtaataaaag ttttataaca
     9361 tcagcagggt taggaactat gggatttggt ttaggtgcag ctattggaac taaagttggt
     9421 aatgtagata aaaatgtagt tttagtcact ggtgatggaa gttttagaat gaactgtaat
     9481 gaactagcta cagttgcaaa ttataatgta cctatgctta ttttactact caataataga
     9541 acattaggaa tggtaaggca atggcaaaag ttattttcca atcaaagata ctctcaaact
     9601 gacattaatg aaaatgtgga ttatgttaaa ctcgtaaatg catataatat tgatggatat
     9661 aaggtatcca gtatggaaga attaggaaag gcactagata tgatagattt taataaacct
     9721 gtatttttgc aatgtgatat agataaggat tatgatgttt atcctattgt agcacctaat
     9781 gatgcattgg aaaatttgat atgtaactaa aacttatact gaatattgat aaaatttttt
     9841 attaaatttt tattggtatt caggttttgt ttagagatag attggcattt tttatttttg
     9901 caaataaatc aatgactata taattaagaa cttattaaat tttaattata caatttagaa
     9961 tagtattaca acttaagtaa ataataattt tgaataataa tttacggagg tattcatatg
    10021 tttaaacacg ataaagcatt attaaaagaa gtaaaagtag aaagaccaaa tcctcaatat
    10081 gctgttttaa tgcaagaaca gttgggtggt gcaaatggag agttaaaagc agctatgcaa
    10141 tacttgtccc aaagttttag aataaaagac cctcaaataa aagatttatt tcttgatata
    10201 gcagcagaag agcttagcca tatggaaatg gtagcacaaa ctataaattt attaaatgga
    10261 catgatgttg attataattc agttaataca ggcgaaatag aaacacatgt attaactggt
    10321 ctatcaccag tcttaataaa ctcatcagga gctccttgga ctgctaatta cgttacagtt
    10381 acaggggatt tagtagctga cttactttca aatatagctt ctgaacaaag agctaaggta
    10441 gtttatgagt acttgtatag acaaatagat gataaatatg taaaagaaac aatagatttc
    10501 ttacttaata gagaagaagc acataatgct ctatttagag atgctttaaa taaagttaaa
    10561 gatacaggtt caaatagaga ttttggagtt acagaagatt ctaaattata tttcgatttg
    10621 tctactccag ggccaaatca cgatactaag atagatatta atcctccttc ttttgaaaaa
    10681 cctttaaaaa aataatttga tattttttat tttgttttag tagttataaa aattatttac
    10741 tagaagaatt aattatatga aaaattaaat atgtatagtt aaaaatgggt atacacttat
    10801 tataagtgta tatctatttg tttattgcaa aacatataat aaaataacaa atgaggtggt
    10861 attttggaca gtaatagaaa acctaataat ctaataaatg aaaaatctcc atatctttta
    10921 cagcatgctt ataatcccat aaactggtat agttggaatg atgaggcttt taaaaaagca
    10981 aaagaagagg ataaaccaat atttttaagt gtaggatatt caacatgtca ttggtgccat
    11041 gttatggaaa aagaatcttt tgaagatgaa gaagttgctg aaataatgaa tagaaacttt
    11101 gtggcaataa aagtagataa agaggaaaga cctgatgtag atagtgtgta tatgacagtt
    11161 tgccaagcta tgacaggtag tggtggatgg ccaatgacca taataatgac accagataaa
    11221 aaaccttttt ttgcgggaac atatttccca aaatattcaa ggtataatag acctggagtt
    11281 atagatttat tagagaatgt atctgaaaag tggaatacaa gcagggatat attaataaaa
    11341 tctggagatg aaataataga agctcttaag gatgattttg gtgtaaaaaa tactgagggt
    11401 gatttatcaa aagagatgtt gagttcatca gttagagtat ttaaggctat ttatgatgaa
    11461 aagtatggtg gttttggtaa tgctcctaaa tttccaagtc ctcagaatct tatgttttta
    11521 atgaaatatt atagtataga aaaagacaaa gatgtgttaa aaatggtgga aaaaacttta
    11581 gatggaatgt atagaggagg cttgtttgat catattggat ttgggttttc aagatactct
    11641 actgataaaa agtggttggc tcctcatttt gaaaaaatgc tatatgataa tgctatgctt
    11701 actatagctt ttttagatgc gtataaaata acaaagaaag agttgtataa agaaattgct
    11761 ataaaaacta tagattatgt agttagagag atgaaggata aagaaggtgg gttttattca
    11821 gcacaggatg ctgatagtga aggtgaagaa ggtaaatttt atacatttaa tcctcttgag
    11881 ataattgaag ttttgggtga agaggatgga actttcttta ataattattt tgatataact
    11941 agtagtggaa attttgaagg caaaagtatt cctaatctca taaaaaataa agaatatgaa
    12001 agacataatg aaaagatagc tgaccttagt aaaaaggtat ttgaatatag aaaagaaaga
    12061 acatctttgc ataaagatga caagatactt acatcatgga atgcattgat gatagttgct
    12121 ttaacaaagg catatagtac tttaaaaaat gacatatatt tagagtattc aaataagtgt
    12181 cttaacttta taaataagaa tcttgtaaat gagtcaggta gattattagc tagatataga
    12241 gatggaagtt ccgactattt agcatattta gatgattatg cttttttaat ctgggcgtat
    12301 atagagcttt atgagtcaac atttaatatg aaatatttag aaaaggcttt aaatttgaat
    12361 gaaagctgta tcaacctttt ttgggattat gaaaaaagtg gattttatat atatggtaaa
    12421 gatagtgaaa atttaatagc aagaccaaaa gatttgtatg atggagctat tccatcagga
    12481 aattctgttc aattatacaa tcttataaga cttgcgaaaa ttactggaga taatagactt
    12541 gaagaaatgt catataaaca attaaaactg tatgtggata atgtaaagag ttctccaact
    12601 ggctacagct tttatatgct ttcattgatg tttgaacttt attctactaa ggaaattata
    12661 tgtattttta aggaagattc tgatttaatt gcatttaaag aattgataag tgaaaatttt
    12721 ataccaaatg caactttttt agcaaagaaa tacaatgaag aaaacactat tataggtttt
    12781 ttaaataact atagattgaa ggatgataag attagttatt acgtgtgtca aagtaatagt
    12841 tgtagtcaac caattaatga tttacaaaaa ttaaaagata tgattttagg agtagaataa
    12901 tcataatagt gaaaaaggag atgctttaaa ataaaagtct aatcatctcc tttttttgta
    12961 caaaaaacag aaaaagagtt cctgagaaca cctaatctat gtaatgattg ttttgttaaa
    13021 aaataaaaat gacattatag ttatataaaa tattgtacta caaaaaagta gtattataga
    13081 tgcgatagta ttaatcgttt ttatttactc tatttcttct ttttgcaaat tcttcaagtg
    13141 cttcagattt aattttatct atttcttcat attccataaa attagcatct tcttcaaagc
    13201 catcttgttc ttcaccatta aaacgataca ttttttttag taaactccat aaggtctcta
    13261 attcatcttt ggtaaattcg tgaaatacat ctgccataaa gtttattcca gtcctactac
    13321 atgtcaccat aacctttttt cctaagtctg taactttaac attaattgct ctcttatcat
    13381 gaggacttgg aattacatca acatatccat ttttttcaag attagcaact agtctattta
    13441 tattttgttt acttgtaccc atttttcttg caatattatt taatgttgtt tcttcctctg
    13501 gtaaatgaag aattgataaa atagtcatat actgtcttga agtaagtata ccaaaatatt
    13561 tatctccttc tatttgtatt ttgtttgtaa gggtaaaaag agttgcatat gtttgattca
    13621 ttaaaaagag ctcttttagt tcatttgaat aatccatgaa atatatctcc tttattttta
    13681 gaagttgttt tgtcgaaaac agaatatcat gaattaattc taatgtcaat atggtgtcaa
    13741 aatatattaa agtattactt ttttatgtaa ctattaatta attaaattat tgtataattt
    13801 tatgtaaaaa cttctatgta ccaatgataa tttctatgag gaattaataa ttcttaaatt
    13861 ttttagaaag attttgactt gaaaatactt gtaatataat atataaatta atatactgaa
    13921 taaaatcaat ctggagggat aattatgaag tgtacaaact ttactttcaa aggagaggaa
    13981 ggtttagata tatatacata taaatgggaa gatgaaaata taaaaaaacc aaaggcagtt
    14041 atccaaattg cacatggaat ggctgaaact gcacaaaggt atgagacttt tgccaaggta
    14101 cttactaaaa atggttatat agtatatatc aatgaccata gaggtcatgg aaaaactgca
    14161 aagataatag aaaatgttgg acatttagct gaaaaagaag gatttagatg tcttgtagag
    14221 gacatgtata ctttaacaaa tattataaag aaagaaaatg aagatttgcc aatttatcta
    14281 tttggacata gcatgggttc atttgcaagt caaagatata taatggatta cagtaataat
    14341 ttatctggac ttatactatg tggttcaaat ggaaagcaag gaatcattct aaatcttgct
    14401 catctgataa tcaatcgtga gattaaaaag tatggaagac gttttaaaag taataaaata
    14461 aataatttga tatttggcgg cgaaataata agaagaaatg aaaagactaa gtttgactgg
    14521 ttaagtagag ataaagaaca agttgaaaaa tatataaatg acccattttg tggagtagtg
    14581 tgtagttgtg gcttttttta tgatttagtt caagggttaa aagaaattga ggataaagaa
    14641 aacttaaaaa aagtgccact tgatatacct atttacataa tatctggaga taaagacccc
    14701 ataggaaaaa atggtaaagg agtcttaaga ctaagggata gatatataaa attaggagta
    14761 aaagatgtaa cttgcaaact ttacaaagat ggaaggcatg aattgttgaa tgaaataaat
    14821 agagaagaag tttttgagga tataatttgt tggttaaata ataaaataga aatattgtaa
    14881 taaaaaaata taatgatata tttactttaa atttatttat atataatatt tgaaaagtgg
    14941 attatgtata tgcaaagtag aaaagagaga gcctagaagt gatttttcgc tataatgcca
    15001 tttattgaat aggtgggtgt tattataaat aaattattaa cactcagtat tattatcatt
    15061 gtatatggaa tttattttat ttaaaattcc ttgaaagttt aaatatagta agttgtaata
    15121 aagagaaaat ctaaatattt aaatgctatt taatgtgaat ctattaatta ggttatactt
    15181 aaacttaatt aatagatttt atatttcaag acaatgagat agggttttag attaaaatat
    15241 aaccaatggt taaattacag acattttgtt aactttccct cgatttacta gtaaatattt
    15301 ttttaatata cttaaattac aacagatgta ctagggaggt atatatgcaa attaaaatag
    15361 gtaaagtcat acagcgtctt agaaaagaac gaaatttaac tcaagaacaa ttagcaaagt
    15421 ttataggtgt gtcaacacca gcagtatcta agtgggagag tggaaattct tacccagata
    15481 tagaattatt gcctttatta gcagactttt ttaatgtgtc tattgataag cttttaaatt
    15541 acaagattga tttaagtgaa gaagaagtaa tgaaaattta taaagaatta gaatcaggct
    15601 ttgctagaat tgaaattgat ttatcaacag aagaaccaaa agaagaattt agacaagact
    15661 tagaatctgt aaaaaagctt tccaatatgt atatagagaa gtatcctaaa agctatttat
    15721 tgaagctaag gatatgttcg ctgtatcaga tgtattcata taaatttggt aagagtgaac
    15781 ttaatgacag agtaaaagaa acaacgaata ttcttgaaga tatagtgaga aatacagatg
    15841 atatccaaat aaaagaaaca gctcttatta tattatcaaa cgcatattgt atgttggagg
    15901 attatgaaaa ggcagagttg tatttaaata tgatacataa gtcaataggg gatacaagtg
    15961 ttaatttagc tatgatatat ttaaagcaaa atagacttga agaagcagaa atattgcttc
    16021 aaaataaatt gtttagtaat gtatttaata taagcatgga ttgtaaggga ataattaatg
    16081 tatacaaaaa tcaatacaag gaattaaaga aaaaattgga gaatagaaat tttaataaaa
    16141 atgcaattga gagagaaatg gagtatataa aaaataagtt gctggggtat gcaaatcttt
    16201 ctttagaaat aaaaaaaatg ctcagtgaag ataaaggtgc attttttagt atgtatatgg
    16261 attatatgga attatctcta atttttttat tttttaacat
//
LOCUS       DAEMIK010000094        16229 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEMIK010000094 DAEMIK010000000
VERSION     DAEMIK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.094"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            273..815
                     /locus_tag="KPK87_002514"
     CDS             273..815
                     /locus_tag="KPK87_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2538657.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            1143..2636
                     /locus_tag="KPK87_002515"
     CDS             1143..2636
                     /locus_tag="KPK87_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2538658.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELTDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2710..2943
                     /locus_tag="KPK87_002516"
     CDS             2710..2943
                     /locus_tag="KPK87_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538659.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDITG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            3099..3863
                     /locus_tag="KPK87_002517"
     CDS             3099..3863
                     /locus_tag="KPK87_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2538660.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4148..7327)
                     /locus_tag="KPK87_002518"
     CDS             complement(4148..7327)
                     /locus_tag="KPK87_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2538661.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            7608..9785
                     /locus_tag="KPK87_002519"
     CDS             7608..9785
                     /locus_tag="KPK87_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2538662.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9799..10221
                     /locus_tag="KPK87_002520"
     CDS             9799..10221
                     /locus_tag="KPK87_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2538663.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10629..11432
                     /locus_tag="KPK87_002521"
     CDS             10629..11432
                     /locus_tag="KPK87_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2538664.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            11425..12093
                     /locus_tag="KPK87_002522"
     CDS             11425..12093
                     /locus_tag="KPK87_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2538665.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            12329..12991
                     /locus_tag="KPK87_002523"
     CDS             12329..12991
                     /locus_tag="KPK87_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2538666.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAIGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            13051..13848
                     /locus_tag="KPK87_002524"
     CDS             13051..13848
                     /locus_tag="KPK87_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2538667.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            13966..15156
                     /locus_tag="KPK87_002525"
     CDS             13966..15156
                     /locus_tag="KPK87_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2538668.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            15265..16014
                     /locus_tag="KPK87_002526"
     CDS             15265..16014
                     /locus_tag="KPK87_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2538669.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         6326 a         1694 c         2670 g         5539 t
ORIGIN      
        1 cttaaactta ttaaaatagc atttggttaa aggaaaatag atattgatat atttatcata
       61 tatataaatg attaattaaa caattataaa taataagtat agtataatat ttaaaaaata
      121 tagatttatg ataaaataag acaaatatgt taaaaaaatg ataatcacgg agaactttta
      181 ccttcttcaa ttaatatatt attattagta ataactttaa aattatatta ctattatata
      241 aaaattggct taaaagaagg tgaaaaaagt aaatgaaaat atgcataaca gtaggtcata
      301 gtatactaaa aggtggtaaa agtacaggtg taaatggtat agttgatgaa tacaattata
      361 ataaaaaatt agctcctatg ttagctgaga tgcttataag tcaaggtaat acagtagatg
      421 taataatttg tccagaaagg cactttatgt cagaaagaga agaatttttc tatagagttc
      481 caaaagtaaa cagtggtaaa tacgatttat tagtagagct acatttaaat aaagctgatg
      541 gaacacaatg tggtacagaa gtgctttact atggcactga aggattagaa tatgctaaaa
      601 gagtatctaa tagacttgga gaattatttg aaaatagagg agctaaaaaa agagaaaatt
      661 tatatatatt gagaaataca aatccagttg caatacagat tgaaagtttt ttctgtgata
      721 atgtgaatga ttgtaacaaa gcaaatgaat caggttatga ttatatagca cgattgatta
      781 cagaaggtat attgaataaa gatataaata tgtgagatta tggtgattta ggtattgatt
      841 aatacctttt aatattagat ataaataaaa atacaatgta ttagtataat ttaaactttc
      901 aaaagataga ttagtttgat tattgacaag agatacctcc ttgaaaatat tgatttatca
      961 gtatatttaa ggaggtattt aaatataaaa atttaattaa tttgctacta aactataggt
     1021 ttatagagta tatactttta tcttcagtct aataaataat ataaaaatag tagaaccttg
     1081 tattataata atataatgta gaaaaatata tagataagat aaatataata aggtggtaat
     1141 atatgaaata tacatttgaa aaatttaagt taaatgaaaa gatacttaag tcattgaaga
     1201 gtttaggata taatatacct tctagagtac aaagagaagt tattccaaaa ttattaaaag
     1261 gtcaaaactt agtagttagg tcaaaaacag gaagtggaaa gacagcaagt tttgcaatac
     1321 ctttatgcga gaatataaat gtagattaca ataatattca agctcttata gtggttccaa
     1381 caagagaatt agccttgcaa gtaaaagatg agatatcaga tatagggaga ctaaagaagg
     1441 tgagatgtag tgctatattt ggcaaacaat ctataaaaga ccaaatagct gaattgaaac
     1501 aaagggttca tattgttgta gctactccag gcagaatact tgaccatata aatagaggtt
     1561 ctattaagtt ggaaaatgta aagtatttag taatagatga ggcagataaa atgtttaata
     1621 aaggttttgt tgaacaaatg gaaaaaatat tattaaattt acctaaggag aaaatagtaa
     1681 gtcttttttc agctacaata gatgaagaga taaaatatat atgtgaaaaa tatatgctag
     1741 attacagtgt tataaatata gaagaaaatg agagtgatac taatcaaaaa acaagacaaa
     1801 tagatgacaa aataatcaag gcaaatggaa gagagaagta catcctttta aaagaattaa
     1861 tatacagcga aaatccaaag agtgtgataa tattttgtaa taccaaagag aaagtatcta
     1921 aattatataa taagatgagt aaagagggtt ttttaataag agagttacat gcagatttat
     1981 cacaagaaag aagaattttt gtaattaagg attttaaaaa ccaaaaattt aatatattag
     2041 ttagtagtga tgtagcgtcc agaggaatac atattgatga tatttcttta gtaataaatt
     2101 atgatgtacc acaagataaa gaaaactata tacatagaat aggtagaact gggagaaaag
     2161 gaaatagtgg gaaggcaatt actatagtaa ctgaaaagga tgaaaaatat atagagaata
     2221 tagaaacata tataggatat aaaataaatg aattaacaga tatagaaaaa agtagaatta
     2281 cacatggcaa agttttgttt gaagaatatt caaaaggaat cctaaaaaaa gtatcaaaga
     2341 gaaaaaaagt tgataaaaat tcttacaaaa gtaaagatat aaaactaaat atagaaagtg
     2401 aagttgttaa attatattta aatgctggta agaagaagaa aataagagtt ttagatatag
     2461 ttggtgcatt tagtaatata aaaggtataa ctaatgatga tataggtgta attgaagttc
     2521 aagatttatg ttcatatgta gatatattaa attataaggg agatttgata ttaaaaaaat
     2581 ataaggagat acctataaag aaaaaaatgg ttaaagtgaa aagagatata cgataattta
     2641 gtcaatattg atgaattgct ttgttatcga aatgaagttg caaaaaacac aatagaagtt
     2701 gttaaggatt tgaaagctga agatatgaaa cgaaaggtct ctccaattga cttagaaagg
     2761 attttggtga ctggaggagt aataaagcat gaagattcta tttggttgtt ggacttttgg
     2821 gggaataaag atattactgg aattttgctt atgcctccta ctcgtcatgt tatacttcat
     2881 ttgaatgatt gttgtaaatg gaaggaatat tttcgtacaa aaaagaaatt ttacagaagt
     2941 taaattataa taaattagac aaacaataga taaattagca aggaatgttg attttatcta
     3001 cattctaagt tatactattt ttaagtggct aagggaatag tcattttaaa tataagtaat
     3061 aaatatatac ctatagggat attaaagggg ggaaatatat ggcatatttt aactataacg
     3121 acaaacagtg ctactataat gaaattgggg atggtacacc attacttttt ttacatggaa
     3181 atacagcctc ttccaagatg tttaatgata taatcgattt ttataaagat gaatataaag
     3241 taatattaat tgattttgta ggtcatggaa aatctcaaat ggtcgataaa ttttctgctg
     3301 acttatggtt tgatgaggct atgcaagtga tatgtttttt agaagcaatg aactataaaa
     3361 aagtaaacat aattggtagt agtggaggcg ctcttgttgc attaaatgtt gcacttgaga
     3421 gacctgattt agtgaataag gtaattgcag atagttttga aggagaagtt cctctagagt
     3481 catttgtaca aaatgtgaaa atagagcgtg aagcatctaa gcaagatgat ggtgcaaaag
     3541 ctttttatat ttataatcaa ggagaaaact gggaaaaagt tgtagataat gatacagaag
     3601 ctatttttga acattataaa actataggaa agttttttca taagcctcta gaaactatgc
     3661 aaccagaggt gcttttaaca ggaagtagag aagatgagtt tgtatctcta atatctagtg
     3721 atttttttga aaatactttt tcattacttc ttgaaaagat taaaaatggt aaaatgtatt
     3781 tgtttgataa aggtggtcat cctgcaattt tatcgaatgg attagatttt tcaaatgtag
     3841 caaagaaatt tttagaggaa taatttaacc aaaatttaca ataatgaaaa ctgtaaaatg
     3901 gattgcaaaa cttacaaaag tgagtgtaca tatacttcat tattatgatg aaatagtatt
     3961 gttaaaacaa aatattctaa tagtcaagtt attacatttg tatatgcaat atgtatagaa
     4021 agagatatca ttagattgta aagttgggat taaataacaa tattaaattt tatttattca
     4081 gatattttag cttaactata taaatgttag aacaaattta tatagttaag ctgtaggatt
     4141 aaataattta gttaaataag tttattatct cctcatcaga tagacttttt aatacacctt
     4201 cattagaaag ttctccattt ataaattgat ttataagctc ttttttactt tcttgtaatt
     4261 ttattatttt ttcttctata gtccctttag caatcaattt tataacttgt actgaatttt
     4321 tttgaccaaa tctatgagct ctatcactag cttgattttc tactgatgga ttccaccatg
     4381 ggtcaaagtg aattaccatg tcagcagatg taagatttaa accagttccg cctgctttta
     4441 aagatattaa aaaaactttt ttatccatac tattattgaa ctcatctaca agttcaagtc
     4501 tttctttggc attagtttta ccatctatat aatggtattt aattttatac ttatcaagtt
     4561 ccttacttat gttcttaagc acagaagtaa attgtgaaaa caataatatt ttgtggtttt
     4621 cgtttatact atccctaagt atttctaaac atgtttcaat ttttgaactt tttttattgt
     4681 aatcttttac tactatactt gggtctaaac aaagttgtct tagtttagtt aaataagaaa
     4741 atataactat tttatctttc tttagatttt tatctttcat tttatcttgt atatcttttg
     4801 aatatacact atatatcttt ttctgctctt tattcaattc aacaaaaaag tttttttcaa
     4861 tcttatctgg aagttctttc ataacttgtt ttttacttct tctaagaata aaaggtttta
     4921 tcaatttttt caggttttta acattatctt ctttatttat aaacaattcc tgaaactttg
     4981 ctttactata tagatagcca ggcattataa aatcaaatat agaccataat tctagtagat
     5041 tattttccat aggtgtacca gtgagtgcaa atttattttc tgcatttata ctttttacag
     5101 actcagttac taaagctact ggatttttta tattttgagc ttcatctaat atacaataat
     5161 caaactttat ttcactatat ttttctaaat catttctaag agtaccatat gttgtaagta
     5221 ttacatcaaa attttctaat tccataaaac atttttctct atctctttta tttccatgta
     5281 tcaataaaac ttttatatct ggagcaaact tttcaaattc atttttccaa ttataaatga
     5341 gtgatgtcgg tgtaacaata agactttttt tattagataa tgataataaa aatgcaatag
     5401 tctgtattgt tttaccaaga cccatttcat cagctaatat accaccaaat ttataataat
     5461 ctaaaacttt aaaccaattg aggccattta tttgatagtc acgaaggttt gcatttaaat
     5521 ttgcgggaat ttctaaattt atgctatcaa aatttctaaa tttttcacat atactgtcta
     5581 gttctttttt accatctata aactttaaat ttttactacg tattacatca tttatatata
     5641 gagctttatt tttatgaatt ttattatttt ttgatttatc cattatatct agattttcaa
     5701 ctaattcaaa aaaatctttt gtttctcttt ctgataaatc tataaagttt ccatctttaa
     5761 gtttataaaa agtacgtttt tcttgaaaag ctttcaaaat atccttgtac tcttttggat
     5821 ttatatcaga tatattaaaa ttaaattcta ggtaattctg gttagtctga ttgattgaag
     5881 cttgaattga tgaagcgtta tatatttttt tcaatttaaa cttctcagaa taatatatat
     5941 ctccaaattc ctttagagat tctaattgta aatctaaaaa tgtaaatagg tcatggtcag
     6001 ttcctttaaa cacataatga ttattttcct ttgtaaattg tgaagaagta agtttatttt
     6061 caatagtagc ttccttttgt atgttgggga gcacaaattt tttatcttca tcatcatata
     6121 tatactttac atcacaacat atgttgccat caatcatatc aaagtagaac tcagatttaa
     6181 aatcttttgt tatattattt tttatgttat catctaaatg aacttcttga gatatagatt
     6241 ttaatctagg aactagcaat cctaaaacat ccttagaatc ctctttatca aattttaact
     6301 ctttagtctt tgataatatt tgatatattt ttgaataatc atgagctttt ttatgtgata
     6361 ataaatagat attgccatca tataaaagta catcattttt tttagtaagt ggaacaggta
     6421 gtgaatcttt agaatataag actattttat cttctatatt tttaaaatca aaccttacag
     6481 gtagagtatc atttaatatc tttggatgat attcctcttt agaaaaaata agggtaaaat
     6541 ccttgtactt aatagcattt aaaaattgtt ttaaagagct tgcatttatc tccataaatt
     6601 tttttgaatg tgatgaagaa aagttgtatg aaagatttgt cccatattct tctataatat
     6661 ctactaactt ctcatcttca tcagcaaaat aatgagtgtt ggggttgtaa gtaaatttct
     6721 tcccaaaatc taattcatat ccattaagtt ttgaatatgc aaattcagta atgtttttta
     6781 aaatgtacat tttatcaatt cctattttaa attcagcaat tgctttattt gaatccacat
     6841 ttatcaatac atctaaatta accctttctc ttttagtatt gtctaacaaa gaaagtaatt
     6901 gcttgcttaa atctaagctg gttttagaat ttgtattaga aagcttatcc ttatctgcac
     6961 ctaatatatc tattagaata ggaggaacat cttttttatt ttttatttgt tttaagcttt
     7021 ttatcacaat agcaactata tgcttacata taaaaatact tttgcccttt gattgacagt
     7081 cagcgcaact acaatcattt tcaattatct taaagtgctt tatatctata gttattgaat
     7141 taaaataaaa gctataatta tcttcagaat tgactccacc acgaaatgtc aacaggttat
     7201 tttgtaggtc atcaatatct atgtctatat aatcaactaa ttgattagta taatatttta
     7261 cacctcttga ccatcttgat ggttctgtat ttcttcttat tagtttatat atcatatcta
     7321 ttttcatatt attcacctta cctcactgta tatattataa tctaaatgca ttcaatttac
     7381 actctaaatt taagactaaa attaaggcag atatgaatag cttttatcta tcacaatata
     7441 tgtgtaaata acaatttact attagatttt tattgaatta tcttataaac tatgaatgaa
     7501 aaataattat ataaatagat tattaataaa tataaaatct tattattaaa aatattctta
     7561 gaaattacac tatataaaat ataaagtatg atatttgaat aaaaagaatg tataataagt
     7621 ataataattt gtatttatta ttgggaaagg aaaatgttaa tatggatagt tatagacaac
     7681 ttaaagctaa aataccctgt aaggaaacag gtatagaaat aaagaggact ttatgtgata
     7741 tttgttctcc aggagtacat tgtggaattg atgcatatgt taaagatggt aaagtaataa
     7801 aaatagaagg tacaaaagaa catccaatga ataaagggtt gttatgtacc aaaggtcttt
     7861 gcaatagaga atatatctat agagaagata gaataaaaac acctctaaaa agaattggaa
     7921 aaagaggtga aggaaaattt aaaccaatat catgggatga agcttataga gaaatagcag
     7981 aaaaattaaa tagagtaaaa tctgagtatg gagcaaagca tgtagctttt tttacgggtt
     8041 atggaaagtg gtatagacct tttttacaaa ggtttgcatc ttcatttggt tcacctaatt
     8101 atggtacaga atctagttca tgttcaacat cagcacagat ggctggaaat gttgctactg
     8161 gaagaaactt tacaatgcaa gatatggaga atgcaaaaac ttatttaggg tggggattta
     8221 atccatacta ctctaaattt ttaaatagtg tgaatctaat aaaattaaaa gaaaaaggtc
     8281 taaaatttat aattgtagac cctagaataa caccgacaac tcaaaaacta gcagatttac
     8341 atttaagaat taaacctgga actgatggag ctttagctct tggaatggcg aatctattta
     8401 tacaaaagaa tttaatagat agagaataca tagaaaagta tgtacatgga tttaatgaat
     8461 ataaagaata tgtaaaatta tttgatttag atactgtaag tagaattaca ggagtttcta
     8521 aagataatat agtaaaagct gtcgagatgt tgtcacaagg tccattttgt ataaatgaaa
     8581 gtgcagctcc aatagttcat catcaaaatg gatttcaaaa ctatagagca atgatgtctt
     8641 tatccgctat aacaggtaat tatgatagaa taggtggaaa cataccaaaa caagattact
     8701 ttgctggtaa gataggtgga ttccaaacac tagaaaaaga gtttgtaaaa gaagttctac
     8761 ctaaaaatat aaatgaaaga attggtggag aaagatttcc tctatggaat gaaatggtga
     8821 ctcaatttca agctatggat ttacctaggc aaatagaaga aaagacacca tatgaaataa
     8881 gagctttgtt ttgtcatggg ataaatgcta gaatgtttcc taattcaggg agacttttta
     8941 aagcgttgaa tagtgtagat ttttttgtga atatagattt atttatgaca gatacatcaa
     9001 aatatgcaga tatactttta cctgcatgta catcatttga gagagaagaa tgtaagagtt
     9061 atccaggagg gtatattaca tataccaaaa aagttattga taaattatac gattcaaaat
     9121 cagatgtaga aatactttca gatttagcaa atgctatgaa tatagatgat gatttattaa
     9181 aagcaggtta tgaagcaagt gtaagacata tgtttaaaaa tacatgtgta gatgtagata
     9241 aacttaaaga aagtgattta ccattaaaat gtaaagattt taagccatat atagtaggtt
     9301 catatacaag agaagggtat gatactagca cttctaggtt tgaattaaaa tctacgatta
     9361 ttgaaaaata taaagatttt gggctggatg cactaccaac atatagagga tttaatgata
     9421 atatagatga tgaatatact catttattga catcaggagc aagaatttca aatgcactac
     9481 attcaagact tcatgacatc aaactttcaa gaatattaaa aaatgaagct agttgtgata
     9541 tttctagctt agatgcaaaa gaattgggtg ttatcagtgg agattatata aaaataacta
     9601 ctaagatagg aagtttggat gtaaaagtca atgttactga tagagtctta tataagaata
     9661 tacatatgta tcatggatat agagaagctg atataaatag tattatttgt gataattttg
     9721 acccttatag tggattccct gcgtatagaa gttgtaagtg taagattgaa aaaatgaata
     9781 gataaatgga gagatataat gaaaagttta tttaaattta ataaagaatt atgtacagca
     9841 tgtggagcat gtttgcttgc atgtatagac caaaatgata ataatataga caatggtgaa
     9901 ctttttagac atgtatttac aatagaggaa ggtgaaaata tagaatattt ttcattagca
     9961 tgtatacatt gtaatgagcc aaaatgttta ggtgtatgtc ctaaaaattg ttttaaaaaa
    10021 gaagaaggat tcgttgtatt agacaatcaa aactgtatag ggtgtaaact ttgtgaaaaa
    10081 gcttgtgaat atggagcctt aatttatgga aatgatggta aggcaaataa gtgtaatggt
    10141 tgtattgaga gatataagtg tggtatggat tcaccttgtg taatggtatg tcctacagga
    10201 gcattaaaat ttaataaata aattaaatat agagaatgac ttatacttag gtataagata
    10261 tttttacaaa aagctgtatt gagtttgtta taatgaaaac tcaatatagc ttttttatgg
    10321 atatagttgg atactataat gtgattctta gtaatagttt tttgctattg gacaaagact
    10381 gtaaaacata ttaaacttat gttgttcaaa gtcgaagtat aatataataa ctagaaaatt
    10441 ggtttatagt gctataaaag caaatttagt aaggtataaa atcagaggca atcctttact
    10501 ataatatcaa tatttgggag ttctgttatt gcagagctat taaacaatta taatggtaca
    10561 agtgattcaa cctttgaaaa tgtattatat aaaaaattaa aataagatta aaaaggagag
    10621 aataaattat gaaaaaactt aaattaatta ttatgtttgc cttggtatca gcactgttag
    10681 ttacaggatg ttcatcagga aataataaaa ataaagaaga acaaaaagtt ataagagttg
    10741 caacatcagg aacatattac ccttttgctt ttaaagataa agataaactt aaagggtttg
    10801 aagtagattt ttgggatgaa tttgctaaac gaactaattg taaagttgaa tgggtattaa
    10861 ctgattttag tggattattt ggattattag aagctgataa agcagatgtt gtatctgctc
    10921 aacttactgc aacacctgaa agagagaaaa aatatgcttt ttcagatgct tataattata
    10981 gtggaacaaa cattattgtt agagaagatg atactagtat aaaatcaata gatgatttag
    11041 ttggcaagaa agtaggtgtg ggtactggag cagtagcaaa tgaaatatta aaagaaaaat
    11101 acccaaatga tgaaataaaa atagtaaatt attcaagtgc gactcttaaa gggaattatc
    11161 aagacctaga aatgggaaga ttagatgcag tagttgcgca ggatgttgag gctttaatag
    11221 ctataaaaga acaaaaatta aatcttaaaa tgatagaacc tccaattcaa tttggtgcct
    11281 gttcttttgc gcttcaaaaa aatgaaaaag gtgaaaaatg gacaaaagaa attaacgaag
    11341 taattgaaga gatgtataaa gatgggactt tgactaaaat atctgaaaac tggctaggaa
    11401 aagacattac taaggagcct attaatgagt aattttaatt ttgaattttg tctaagtata
    11461 tttccatttt tgattaaagc attgggaata acaatactaa tatctattgt tgcatttttt
    11521 ttatcaatat tattaggtgt aatacttgcc tttttcacac agacaaaatc aaaagtttta
    11581 caatacataa caagagtgta tgtttcattc tttagaggaa caccactttt aattcaaata
    11641 tttttctttt attttggatt tccaatgata ttttctttta tgaaagattg ttcaccttat
    11701 ttagcaatag taatttgttt aggtgtaaat tcatctgcat atatggctga aattatacga
    11761 gcatctattg agagtgttga taaaggacaa caggaagcat gttatgcttt tgggttaagt
    11821 aaatatcagt caatggttaa aataattcta cctcaagcag ctacatctgc agtacctcct
    11881 attgtaagct gtacactgga cataattaaa ggttcttctt taggtttaac tataggaata
    11941 caagatttaa tgggagtagc tcaagtagag gctgcttcaa gttttaaata ttttgagata
    12001 tatatgataa cgatattaat ttactgggca gtatctataa tattgagtaa tatacaaagc
    12061 aaagttgaaa aaagaataag aagagcatat tagatttcaa tatttaaaaa tgggatgata
    12121 tagatatatt attacttgta aataaatttt taattttaga aaaataaact tagtaatcta
    12181 aattattgag aaaatatagt atcaaaatgg actgtataat caagttaaaa aatagttttt
    12241 ctattgaaaa aataaataaa attctattgt aatttaatat gcgataaagt tttataaaaa
    12301 acaacattaa gctaggggga cttattttat ggcttttaat atgaaatatt ttttagaatt
    12361 atttccaaca ataatgaaat cgttcaatgt aactgtgatt ttggcaataa gttcattagt
    12421 attttcatta attataggta caatagtagc gttatcatct tattacaaag tgaaagtatt
    12481 gaatcaaata tgtaaagtat atgtatctat ttttagaggt acacctctta tgccacagtt
    12541 attcttttta tattttggac tagcatatat gagtgatttt gttaaaaata tggacccagt
    12601 atttgcaaca tctatagtac ttagtttaaa tatgggagct tatatgtctg aaacgataag
    12661 ggcatctata ctttcaattg atacaggtca gatagaagca gcatatgcaa taggattaac
    12721 aaatttacaa acaatgaaaa ggatagtgat accacaggcc gtaagagttg cactgccatc
    12781 attattcaat aattttatag atttaattaa agggtcttct gtagcttttg ttgttggagt
    12841 aaatgatata atgggtgctg ccaaatcaca aggagcgttg agttttaagt tttttgaagt
    12901 gtatggagca gttatggtaa tttactggtg tataataact cctttaggtt gggggcaaaa
    12961 aaaattagaa aagaaaataa gcaattatta agttattaag aaaatacata aaagtatttt
    13021 tatataaaaa atggtgtgag gagaagagat atgataaaaa gaaatattat aaaatgttta
    13081 gtggcagttt tatcgttaac gtttgtaatt actggttgtt caaaaaaaga ggaagtaaag
    13141 aatgaaaaag ttataactgt agcttcatct aattcagctg acccatattc ttatgttgat
    13201 aatggtgagc ataagggttt tgaagttgac atgtggaaag agattggtaa aagaagtgga
    13261 tataagataa aaatggaacc aacaggtttt agttctatat ttggtatgtt agattctggt
    13321 aaagttgatg tggcagccaa cttctttgga atgtctaaag aaagattaga aaaatacgat
    13381 gcttctatac cttatggttc tgattctgtg tgtatagctg taaaagatgg aaataacgaa
    13441 attaataagc ttgaagattt aaagggaaaa gttgtagcag tttcagaagg ttctcaaggt
    13501 caacaggttg caactagtgt taataaagat aatttgtttc aagaaaaggt ttacggtgat
    13561 ggaactagtg gtcttcaaga tttagatttg ggaagagtta gtgcatggat tgatgcagag
    13621 cttactattg tgggagacgc taaaaaagct aatatgaaag taagagtatt agataaaaaa
    13681 ttatcagcta ctaatatagc atacttcttt aagaaaaacg atgataaatc taaaacgata
    13741 aaagaagatg taaacaaagc aatagaagaa atgcttgcag atggaacagt gaaaaaaata
    13801 tctgaaaaat ggtttggaat ggatgtaagt gctgatatac aaaaataaat actttaaaat
    13861 taaaatgtaa aactgtgata actatagata agtatactaa atttatctat agttatctgt
    13921 gtattacaat gtatttagat tagtctttat aaatcaaaat gagttatgga ggtattgcaa
    13981 atgattgaaa ctaaaataaa atccattgca ctaaaaaata gagaaaaaat tataaaaata
    14041 agacgacaga tacacagtaa tccagagtta gcatttaaag aatataaaac atctaaactt
    14101 ataaaagaag agctaaataa acttaatata gaatacataa atattgctgg tacaggagta
    14161 ttagctacta taaaaggaaa aaataatggt ggaaaaacta tattactcag agcggatatg
    14221 gatgctcttc caatcaaaga ggaaaatgat ttagaattta aatcaataaa tgataatatg
    14281 catgcttgtg ggcatgatgc acatgtttca tggttgttag gaacagcaat gattttaaat
    14341 gacataaaag aagaattgaa tggaaatgta aaattactat ttcaacctgg cgaagaaaaa
    14401 ggtggttctg atattgtaat taaagaaaat gtacttgaag gtgtggatgc attagcgact
    14461 ggtcattgtt ggccaactat tgagtcagga aaaattggaa tcgctagaaa ttgtgctatg
    14521 gcagcgacta atacttttga aataacaata attggcaagg gtgggcatgg agcagagcct
    14581 cataattgta tagacccaat tgcagttgga aatgcagtat attcatccat tcaacagata
    14641 gtttcaagaa aaattgaccc agttgtacct gtagtagtgt ctgtttgttc ttttaattct
    14701 ggtgtttcta aaaatataat accagatgta tgtacacttc aagggactat tagagcgata
    14761 agcaaggaac aagtaattga agtaagtaaa attctagaat atatagtaaa aaatgtatgt
    14821 aaagcacatg gagcagattg caaatttgaa aagagtacag gaggagatgc tgttataaat
    14881 gacaaaaaca tgatagagat aggaagagaa agtgcttgca aaatacttgg agctgaaaat
    14941 gttgaaataa ttgattttcc tgcaatgaca ggtgaggatt ttgctattta tatgaaagaa
    15001 aaacctggtt tatttatgta tataggtgta ggagataaag aaaaaaatat aaattacaga
    15061 ttgcatagta acaagtttaa tatagatgaa aaatgtctta gtatagcacc ttcattattt
    15121 tcacaactta cagtagatta tttatgtcaa ctatagagta cattcataaa aatatattta
    15181 gcctattttt aaatttaatg ctagtatatt aattttgatg tagaacatat tatttattat
    15241 atttatttaa aaaggtggat aacaatgata aaagtagaaa atttacataa aagttttaaa
    15301 gggacagaag ttcttaaagg tataaattta gagataagtc aaagtgaagt agtagctata
    15361 cttggatcat ctggaacagg taaatctacg ttacttagat gtttaaatta tttagttgag
    15421 cctacaaaag gaaagataat gattggtgat gtaaaagtag atgtagaaaa agcaacaaaa
    15481 aaagatataa ccaatttaag aaaacataca tctatggtgt ttcaaaatta taacttattt
    15541 aaaaataaaa cagcattaga aaatgtaatg gaggcattga tagtagttca taaaaagagt
    15601 aagaaagaag ctagagaaat atctttaaaa ttaatagata aagtaggtat gttggatagg
    15661 aaagattttt atccatcaaa gttgtctggg ggacagcaac aaaggatagg tatagcgaga
    15721 gcgatggcag taaatccaaa tgtaatatta tttgatgagc ctacatctgc acttgaccca
    15781 gaacttgttg gagaagtttt aagcgttata aaatctcttg caaaagaagg tataacgatg
    15841 cttattgtaa ctcacgaaat aaattttgca aaagaagttg ctgataaaat tatatttatg
    15901 gataatggtg taatagctga agaaggaact cctgatgaaa tatttaataa tcctaaaaat
    15961 gaaagaacag caaaattttt aaatatcatt aggaaagaag aagtatttag ttaaaagtgt
    16021 ataaattaag cattagaaga tatgtaagat acccccatta aatttataag ttagataata
    16081 aatttaatgg gagtatattt ttattattag atttattatt taatttaaaa ttagaaatgt
    16141 aactatttga aaattctttt taaaagtata tcaaaatatc aaatacataa aaaaataata
    16201 tttgacaaag tatcatatgt gtttacaaa
//
LOCUS       DAEMIK010000095        16218 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEMIK010000095 DAEMIK010000000
VERSION     DAEMIK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.095"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..237
                     /gene="tuf"
                     /locus_tag="KPK87_002527"
     CDS             <1..237
                     /gene="tuf"
                     /locus_tag="KPK87_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG2538670.1"
                     /translation="GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            487..636
                     /gene="rpmG"
                     /locus_tag="KPK87_002528"
     CDS             487..636
                     /gene="rpmG"
                     /locus_tag="KPK87_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG2538671.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            662..883
                     /gene="secE"
                     /locus_tag="KPK87_002529"
     CDS             662..883
                     /gene="secE"
                     /locus_tag="KPK87_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG2538672.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            903..1445
                     /gene="nusG"
                     /locus_tag="KPK87_002530"
     CDS             903..1445
                     /gene="nusG"
                     /locus_tag="KPK87_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG2538673.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1484..1909
                     /gene="rplK"
                     /locus_tag="KPK87_002531"
     CDS             1484..1909
                     /gene="rplK"
                     /locus_tag="KPK87_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG2538674.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1980..2678
                     /gene="rplA"
                     /locus_tag="KPK87_002532"
     CDS             1980..2678
                     /gene="rplA"
                     /locus_tag="KPK87_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG2538675.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2730..2858
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2901..3407
                     /locus_tag="KPK87_002533"
     CDS             2901..3407
                     /locus_tag="KPK87_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG2538676.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3465..3830
                     /gene="rplL"
                     /locus_tag="KPK87_002534"
     CDS             3465..3830
                     /gene="rplL"
                     /locus_tag="KPK87_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG2538677.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4222..5010
                     /locus_tag="KPK87_002535"
     CDS             4222..5010
                     /locus_tag="KPK87_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2538678.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5481..9197
                     /gene="rpoB"
                     /locus_tag="KPK87_002536"
     CDS             5481..9197
                     /gene="rpoB"
                     /locus_tag="KPK87_002536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG2538679.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9239..12724
                     /gene="rpoC"
                     /locus_tag="KPK87_002537"
     CDS             9239..12724
                     /gene="rpoC"
                     /locus_tag="KPK87_002537"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG2538680.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13013..13435
                     /gene="rpsL"
                     /locus_tag="KPK87_002538"
     CDS             13013..13435
                     /gene="rpsL"
                     /locus_tag="KPK87_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG2538681.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13559..14029
                     /gene="rpsG"
                     /locus_tag="KPK87_002539"
     CDS             13559..14029
                     /gene="rpsG"
                     /locus_tag="KPK87_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG2538682.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14078..16144
                     /gene="fusA"
                     /locus_tag="KPK87_002540"
     CDS             14078..16144
                     /gene="fusA"
                     /locus_tag="KPK87_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG2538683.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6347 a         2020 c         3308 g         4543 t
ORIGIN      
        1 ggtggaagac atacaccatt ctttgatgga tatagaccac aattctattt cagaacaaca
       61 gacgtaacag gagcttgtaa gttaccagaa ggaatagaga tggtaatgcc tggagataac
      121 gtaacaatgg aagtagactt aataaactca atagttgtag aagagggatt aagattctca
      181 ataagagaag gtggaagaac agtagcttca ggagttgttg ctacaataat agagtaattc
      241 tttactaaac tgatggatta aaaggaataa caaaaagagg ggttatgctc ctcttttttt
      301 aacatagaaa tacaaaaaca ttgacacagc ataggatttt gtgataactt atacaaggta
      361 atgattatag gaagttaata atatcttgaa tactgtagat ttttaataaa tacatatttt
      421 aaagatatta tattcacata tattaacaat atatattatt gtattacaaa gttggaggtg
      481 tatcagatga gagttaaagt aactttagca tgtacagagt gtaagcaaag aaactacaac
      541 actactaaga ataagaaaaa caatccagac agaatagaat tacaaaaata ttgtagattc
      601 tgtaaaaagc atacaactca taaagaaaca aaatagtttt ttataaggat gtgagtatgg
      661 aatggctgcc caaaaaaatg acggtgcaaa aactaaaaaa aggtttagtt tgtttggata
      721 cttaaaagaa actaaacaag aattaaaaag agttacatgg ccgacaaaaa aggaactgtt
      781 taaaaacaca agtatagttt taactgtagt tatttcatgc actatattag tatggggtat
      841 agacactata ttatctggtg cactagcgtt attactaaaa tagacgaaaa gaggtaagtt
      901 aaatgtcaga attacaagaa gcaagttggt atgtagttca tacctattcg ggacatgaaa
      961 ataaagttaa agctacaatt gagaaagcag ttaaaacaag aggtatggaa gactgcataa
     1021 gacaagtagt tgttccaaca gaagaagtag tggaaactac taaaactgga aaagaaaaaa
     1081 ctagacaacg taaagtttat ccaagttatg ttttggttaa aatgataata actgatgaat
     1141 cttggtatgt agtaagaaat actaagggag ttacaggttt cgttggacct ggttcaaaac
     1201 cagtgccatt aagtgaagac gaagtcaaag ctatggggat agacacaaca gaccctaaag
     1261 tagtaaatag cgatattgat tttgaaatag gagatacagt aaaagtatct caaggaccat
     1321 ttagtggaca aataggtaac atagaagaaa ttgacttaga aaatagagaa gtcaaagtat
     1381 gcataaatgc atttggtaaa aggactctat ttgtaataga gcttgaaggt atagaaaaaa
     1441 tctaatacta tattatagaa aaatctgagg aggtgcgctc aaaatggcta aaaaagttat
     1501 aggtcaaata aaattacaaa tacctgcagg aaaggctact ccagcgccac cagttggacc
     1561 agcattagga caacatggtg ttaatataat gggattcaca aaagaattta atgctaaaac
     1621 tgcagatcaa gctggaatga taataccagt tgttataact gtatatcaag atagatcatt
     1681 cagttttata acaaaaactc caccagctgc agttttaatt aaaaaagcat taaacttaaa
     1741 atcaggttct ggagaaccaa acaagaaaaa agttgctaaa atgacttcag ctcaagtaag
     1801 agaaatagct gaattaaaga tgcctgactt aaatgctgca tcagtagaag ctgctatgag
     1861 tatgatagct ggtactgcta gaagcatggg tgttgtaatc gaagactaat taaaagatac
     1921 actttttttt ataaagtggg aggctagaag ccgaaattaa ccacgaggag gaaaataaaa
     1981 tggctaaaaa aggtaaaaga tatgcaggtg cattacaaaa agtagataga actaaatttt
     2041 atgatgcatc agaagcatta acattagttt cagatatagc tggagctaaa tttgatgaga
     2101 cagtagaagc acacattaaa ttaggtgttg actcaagaca tgctgaccaa caagtaagag
     2161 gtgcagttgt attacctcat ggaactggta aaacaaaaag agttttagtt tttgctaaag
     2221 gtgaaaaagc taaagaagct gaacaagctg gtgctgactt tgtaggagct gaagaattag
     2281 ttcaaaaaat acaaggtgaa aactggtttg actttgatat agtagttgct actccagata
     2341 tgatgggtgt agtaggtaga ttaggtagag tattaggacc taaaggttta atgccaaacc
     2401 ctaaatcagg aacagttaca tttgatgtag ctaaagctat agatgaaata aaagctggta
     2461 aagttgaata cagattagac aaaactaaca taatacacgt tccagtagga aaagtatcat
     2521 ttggtggaga aaaattaact gaaaacttta ctgcattaat ggatgctata ataaaagcta
     2581 aaccagctgc tgctaaagga caatatttaa gaagtataac agtagcttct tcaatgggac
     2641 ctggagttaa aataaaccca gcaagaacag ctgaataata aaactgttga cacaggttga
     2701 caaaaatgtt attatatcac ttgtcaatgt aaaaaagaat agatttgccg tagatagtgg
     2761 gtgcgagagc ttaatatccc gccgaggttt tagataaatt gagattacta aagcttttga
     2821 tgtctacttt tggcaacaaa agctttttat aatgttgcgg taaatactaa gcctatttgg
     2881 tgaaggaggt gcacatctat atgagaaaag ctatagaaat taaatcagaa gttgtttctg
     2941 agatagttga aaagttacaa aaatcttctg ctgctgttgt tgtagattac aaaggattaa
     3001 cagttgaaga agtaactgag ttaagaaaac aaatgagaga agctggtgta gattacaaag
     3061 tatacaaaaa tactttagtt agaagagcag ctaaagaagt tggtatagag caatttaatg
     3121 atgaattatt agttggaact aatgccatag catttggata tgatgatcca gttgctccag
     3181 ctagaatatt aaaaggattc atggattctc atccaaaaat gaaattaaag atgggtattg
     3241 tggaaggtgc attctatgat gaatctaaga ttgttgaaat ggctaacata ccatcaagag
     3301 aagttcttat cgctaaatta cttggtagct taaaagctcc agtatcaaac ttcgcatact
     3361 taatagatgc aatagcgaaa aaggcagaag gtcaagaaga agcttaattg cttaactaaa
     3421 aaattataaa tattaatttg aaataaaatt cggaggtgct aaaaatgaca atagaacaaa
     3481 tattagaagc tatagaaaat atgaaagttt tagaattaaa tgaattagtt aaagctgctg
     3541 aagaaaaatt cggtgtatca gcttcagctc cagtaatggt agctggtgca gctgctggtg
     3601 gaccagctgc tgaagaaaaa actgagtttg atgtagtatt aactgacgtt ggttcttcaa
     3661 aagttggagt tataaaagca gttagagaaa taacaggatt aggattaaaa gaagctaaag
     3721 aagtagttga caatgctcct aagacagtta aagaaggagc ttctaaagaa gaagctgatc
     3781 aaataaaaga gaaattagaa gctgctggag ctaaagtaga agttaagtag tttctttaaa
     3841 aagataccca ttgggtatct ttttttattt ataaaaaact ataaagaaag tatttggaat
     3901 tataaagaaa gtatttttat aaaataaaaa aaatttatat aatagtacta aattaagggt
     3961 atcatatatg gtgtaatggt attttaattt aacattatta tcaaattaaa atataaacaa
     4021 ttgttgtaat gtctgtatat aaagtatata attaaactaa aatgtaaaat tttacatttt
     4081 agtttaaaaa aatacacgag tgttttataa ataaaatgta ataaataatt aattaacaaa
     4141 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     4201 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4261 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4321 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4381 ggtggttgtg ctaagtttgt ttactttaat gctcgtgagg aagagacctt tacctcaatg
     4441 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4501 acaaaccctt ctcttgacaa agaccttgta actggagata cagataattt ctttgacaca
     4561 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4621 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4681 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4741 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4801 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4861 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4921 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4981 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     5041 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     5101 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     5161 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     5221 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5281 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5341 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5401 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5461 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5521 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5581 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5641 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5701 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5761 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5821 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5881 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5941 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     6001 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     6061 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     6121 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     6181 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6241 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6301 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6361 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6421 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6481 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6541 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6601 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6661 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6721 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6781 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6841 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6901 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6961 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     7021 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     7081 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     7141 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     7201 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7261 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7321 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7381 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7441 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7501 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7561 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7621 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7681 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7741 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7801 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7861 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7921 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7981 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     8041 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     8101 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     8161 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     8221 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8281 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8341 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8401 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8461 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8521 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8581 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8641 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8701 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8761 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8821 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8881 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8941 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     9001 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     9061 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     9121 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     9181 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9241 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9301 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9361 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9421 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9481 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9541 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9601 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9661 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9721 gtatggatat acttttactg taggaatggg tgctgaagct gtaaagacat tactacaaaa
     9781 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9841 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9901 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9961 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
    10021 tataaataga aacaatagac ttaaaagatt attagagctt ggagctccag atataattgt
    10081 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
    10141 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
    10201 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10261 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10321 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10381 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10441 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10501 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10561 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10621 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10681 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10741 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10801 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10861 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10921 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10981 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    11041 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    11101 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    11161 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    11221 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11281 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11341 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11401 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11461 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11521 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11581 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11641 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11701 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11761 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11821 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11881 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11941 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    12001 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    12061 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    12121 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    12181 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12241 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12301 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12361 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12421 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12481 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12541 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12601 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12661 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12721 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12781 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12841 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12901 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12961 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    13021 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    13081 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    13141 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    13201 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13261 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13321 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13381 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13441 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13501 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13561 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13621 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13681 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13741 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13801 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13861 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13921 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13981 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    14041 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    14101 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    14161 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    14221 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14281 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14341 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14401 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14461 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14521 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14581 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14641 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14701 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14761 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14821 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14881 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14941 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    15001 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    15061 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    15121 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    15181 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15241 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15301 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15361 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15421 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15481 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15541 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15601 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15661 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15721 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15781 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15841 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15901 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15961 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    16021 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    16081 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    16141 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    16201 tctcatctag cacaagat
//
LOCUS       DAEMIK010000096        16112 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEMIK010000096 DAEMIK010000000
VERSION     DAEMIK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.096"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..818
                     /locus_tag="KPK87_002541"
     CDS             <1..818
                     /locus_tag="KPK87_002541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538684.1"
                     /translation="MDEYISNYKFDINLNEIILSLSINQGIYNSKKEHVTSFMISDNN
                     EYIFCTKKAIAYSIDWDKKSVRKKKLSEAKVNDWFLFVDDYLNEEGELVINVIQKLLE
                     LNLLPSHYLDDYKLTNSWKSELKNYISYSKYNYNDVSNSLAVLGEKINPITIKNWVTN
                     KRLIGPIYLKTFEKIFKVIDSKLDHTEVYDATKSIRSLHTKIKKSIDTFVSDNFFSSK
                     SDIIKDEVSAVVLSMLGNTNDYVTPVQIREIHSCDKYLSPHLVNKVLDLYSVY"
     gene            836..4363
                     /locus_tag="KPK87_002542"
     CDS             836..4363
                     /locus_tag="KPK87_002542"
                     /inference="COORDINATES: protein motif:HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBG2538685.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNHEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            4365..6278
                     /locus_tag="KPK87_002543"
     CDS             4365..6278
                     /locus_tag="KPK87_002543"
                     /inference="COORDINATES: protein motif:HMM:NF020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HBG2538686.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(6984..7727)
                     /locus_tag="KPK87_002544"
     CDS             complement(6984..7727)
                     /locus_tag="KPK87_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538687.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(7749..8063)
                     /locus_tag="KPK87_002545"
     CDS             complement(7749..8063)
                     /locus_tag="KPK87_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538688.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(8067..8267)
                     /locus_tag="KPK87_002546"
     CDS             complement(8067..8267)
                     /locus_tag="KPK87_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538689.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            8414..9175
                     /locus_tag="KPK87_002547"
     CDS             8414..9175
                     /locus_tag="KPK87_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538690.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENITIK
                     NNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            9232..9430
                     /locus_tag="KPK87_002548"
                     /pseudo
     CDS             9232..9430
                     /locus_tag="KPK87_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9427..9918
                     /locus_tag="KPK87_002549"
     CDS             9427..9918
                     /locus_tag="KPK87_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538691.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            10010..10762
                     /locus_tag="KPK87_002550"
     CDS             10010..10762
                     /locus_tag="KPK87_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2538692.1"
                     /translation="MNKSYIPKYTHKFPNNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            10752..11918
                     /locus_tag="KPK87_002551"
     CDS             10752..11918
                     /locus_tag="KPK87_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2538693.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(12034..12264)
                     /locus_tag="KPK87_002552"
     CDS             complement(12034..12264)
                     /locus_tag="KPK87_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538694.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(12270..12749)
                     /locus_tag="KPK87_002553"
     CDS             complement(12270..12749)
                     /locus_tag="KPK87_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBG2538695.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(12749..12964)
                     /locus_tag="KPK87_002554"
     CDS             complement(12749..12964)
                     /locus_tag="KPK87_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBG2538696.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(13296..13645)
                     /gene="ssrA"
                     /locus_tag="KPK87_002555"
     tmRNA           complement(13296..13645)
                     /gene="ssrA"
                     /locus_tag="KPK87_002555"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(13803..14258)
                     /gene="smpB"
                     /locus_tag="KPK87_002556"
     CDS             complement(13803..14258)
                     /gene="smpB"
                     /locus_tag="KPK87_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG2538697.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            14871..15698
                     /locus_tag="KPK87_002557"
     CDS             14871..15698
                     /locus_tag="KPK87_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG2538698.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         5946 a         2137 c         2262 g         5767 t
ORIGIN      
        1 ctatggatga atatatatct aattataaat ttgatataaa tttaaatgaa attatattat
       61 ctctatctat aaatcaaggg atttacaact ctaaaaaaga acatgttaca tcattcatga
      121 tttctgataa taatgagtat atattctgta caaaaaaggc aatagcctat tcaatagatt
      181 gggataaaaa aagtgttagg aaaaagaaac tttcagaggc taaagttaat gactggtttt
      241 tatttgttga tgattactta aatgaagagg gtgaattagt tattaatgtt atacaaaagc
      301 tattagaact taatttattg ccatctcatt atcttgatga ttataaatta actaattcat
      361 ggaaatcaga acttaaaaac tatatatctt atagtaaata taattataat gatgtatcaa
      421 acagtttagc tgttttaggt gaaaagatta atcctataac aatcaaaaac tgggtaacaa
      481 ataaaagatt aataggtcct atatatttaa aaactttcga aaagatattt aaagttatag
      541 attctaaatt agatcatacc gaggtatatg atgctactaa aagtatacgc tctttgcaca
      601 ctaaaataaa aaaatctata gatacatttg tatctgataa tttctttagc agtaaatcag
      661 atataattaa agatgaagtg tctgcagttg ttttaagtat gcttgggaat acaaatgatt
      721 atgttacacc tgtacaaata cgagaaatac acagttgtga taagtatctg tcaccccacc
      781 ttgttaacaa agttttagac ttatatagtg tttattaaat aaataggagg taaatatgaa
      841 ttgtagtgaa ctaagagata aaaccctaga tcttgtaaga aaagaacttc ttggacctgg
      901 ttcggaagat ctttgtgaaa atataaaata tgaaataata acagatagtc caatagagcg
      961 ttattcaaca ggtattttat atccacttaa taacaatatt gatgtaagta atacatctga
     1021 atatgatggt gaagataatg tatttacatc agatgataca caaaatgaac tagatagagc
     1081 tataagtatg tctaatgaaa ctttaccttc ttctatgggt atgacttttt tcctaaaaaa
     1141 agatgcagaa gtaataaatt tcagggtaaa ttatgctact tatagaaatg ctttatatac
     1201 agactgtcat gttaaatata ctggtgacga cttctattct agcaatacaa ttaatgaata
     1261 tgtagaactt aggggatcat tcctatatct taagcaaaaa ctaacatctg agaaggttta
     1321 taatttacta aaaaagcatg atttacttga taatgctgaa gctaaatttg ttttatacaa
     1381 gttagctaac caatgccctg atgatgagaa taagactggt ggttatgttc gtgttccaca
     1441 tgaaaactta atagtatcaa tagatatgga taaaaactat attaaaaaac ctatatctaa
     1501 agatttagat atagttttat ctaaaagaat ttattcaaat gactttgtta gtattacttg
     1561 tatgctggtt aatagtcaat ctgggcctaa gaaatataat aatactattt tccagcctga
     1621 actaatggta ttagctgaag ataacaatga tttagagttt atagatattt ttacagctgg
     1681 atcagtaaat acttttaaaa agttagattc tgaggaaata tctttagact tactttaccg
     1741 aaataaaaaa acttttgcta ctggtcatgg tgtagctata gatcaagata ttcaaaatga
     1801 tgctttaata aaactttgta ctgagtttat tcctcatttt gaagtaccta aattggactt
     1861 taatgtagat gaattaagtg atgtttctag agaattactg tctatgaaaa atttatcaga
     1921 tatctctgat cttagtaaag ataagaaaat atattttctt tataagtttg ttgatgctta
     1981 tgatatttgg atagatgatc tagaagaaga ttcaaaagat cttagtgatg tttttaaaga
     2041 tgtagcatat gagcatataa aaaactgcag agatactaag tctagaatgc tagaaggtat
     2101 aaatttaatt aagtctaatg atgaagtata cagagcattt tctctaatga atagagctat
     2161 acttatgcaa agatgtcatt ctatacatat tacggataga ctccctggat ctaataacct
     2221 tgactatata gattactcaa atgataattc tgcttggaga ccattccagc ttgcttatat
     2281 acttacatgt ttatcttcga tagaaaatcc tgactctaaa catagagatt tagttgacct
     2341 tctttggatc cctactggtg gaggtaaaac ggaggcctac ttaggtctat ctgcttttac
     2401 tatcttctta agaagaataa gaaacaaaga aaatggtaac ggtacagcta tcataatgag
     2461 atatacacta aggctattgg ctgcacaaca attcataaga gcaagtatat taatttgtgc
     2521 ctgtgaaatt attagaaaag aaaataagtc tgaacttggg aaaaaagaga tttctattgg
     2581 tttgtggatt gggagtgatc aaactccaaa ctttaatcat gaggcagaag aaatgcttaa
     2641 taacttaaca ggtaataaca ttaagtttag atctagtata aatagtgaat atgagttaga
     2701 taatttaaaa tcaaaatata ataaattcca attactaaaa tgtccttggt gtggaacaaa
     2761 acttgttaaa gaatgggata aaacttctaa aaaagctgtt ggtgattggg gctatagaat
     2821 taaatctaga cctaagaaat tctacttatg ctgtcctgaa gaagattgtt catttgaaga
     2881 tatgttacct atacaagttg tagatgagga gatttataat aaccctccaa cacttttatt
     2941 tggtacagtt gataaatttg caatgataac ttggactaca gaagtagcta gtctttttgc
     3001 tttagatgat gataatagca acttatctcc tgagttaata attcaagatg agcttcatct
     3061 tatttctgga cctttaggta ctatggtagg tttatatgaa actgcacttg atgctatgtg
     3121 tagctataag ggtataaaac ctaagattat agcttctact gctactataa gaaaagcaca
     3181 aaatcaagtt aaaaatctat ataatcgtaa tgtagctcag tttccacctc caggattaac
     3241 atctagtaac tctttctttg ttaaagagat gcctttaagt aaaaaacgtg gccgactata
     3301 tgtaggtgtt attccatctg gaaaaactca agttactgta gaggttagac ttatgtcagc
     3361 attactccaa agacttaaaa tgatggagaa tgtagagttg aatttattgg ataaatattg
     3421 gacactagta tcttatttta atagtatcag agaattaggt aagtgtagca cacttttaaa
     3481 tgacgatata aaagacaaca tggaaagatt atgtaaaaga gtctcagccc acccttatca
     3541 atcattaagg aacctaaatg aacaaagaga gcttactagt agacttactt ctactaagat
     3601 aaatacaact ttagatgaac tatctaatac tcttacttct aataatttaa ctgtcgatac
     3661 tctattagct agtaatatga tttcagttgg tgttgatgtt gatagattaa atttaatggt
     3721 agtagttggt cagcctaagt taacatctga atacatacaa gctagcagta gagttggtag
     3781 aagtaatgaa ggacttatat ttacattata taatcctaca aaaacaagag atagatctca
     3841 ttatgaacag ttctttagtt atcatcaatc attctataaa tttgttgaac ctactagtat
     3901 tacacctttc tctgaaccag ctagagaaag aggcatgcat gcagtattaa taagtatgat
     3961 tagacatatt ttaggacttc aagctaatga tcttgcaagt cgattttcaa aaaatatgga
     4021 tggtgtagaa gatttaaaaa acttcataat agatagatct atcgatatat ttagagaaaa
     4081 taatcaaaat gctgatgata atatagtaaa tgaatttaaa tctgagattt catctgaatt
     4141 agatattata atggatttat ggcatgataa agctcaaata tctgataaag ataatttatt
     4201 gcattattat aagcgcccta gtaaatttga taataaaata tataataatc ttttaattcc
     4261 ttatgataag gatgttatta caaatgaaaa acaaacatta aattctatga gaaatgttga
     4321 taaacaatgt actgttgaaa ttatagaatt ggaggaaaat taatatgtct ataaaattag
     4381 atgataaaac tcataattta agaataaatc aagctacaag tgtttttgga ccaggagcta
     4441 tgctagattt tattgatcaa acattaatga ctgctcatcc aaattattgg actaatacaa
     4501 ttaaaataca agatgatagg cttcaggcta cattgagggt taatgaacta agaacacctc
     4561 caactataga tgatagatct tctgttcctt ttgttaggtt tcctagatgg tacttttgtc
     4621 caaaatgtag aaaatttatg cctatagaag attgggagaa actttataaa aatgctcata
     4681 aagataaacc tatgaaaata cctatttgcc ttacttgtag cacaaaaaag tctactaaac
     4741 aattagtacc tactggtata attactattt gtgaacatgg tcatattgat gattttccat
     4801 gggtaaaatg ggttcatttt agaaataaat ctggaagaaa agaaatttgt gaaaatcctc
     4861 aaatattaat gaagactgga aaaagtaacc taggattaga aggtatagaa cttgaatgta
     4921 gctgtggagc taaagctaat atgaagggcg catttatgcg tttaaaatct tctaaatcta
     4981 atgattatgg tgtacctagt gaatataagg aaatgtttaa atgctctggt aattcgcctt
     5041 ggtataataa aaaagtaaat aaatgcacta aatattcatt tgcttctcaa agaggtgcct
     5101 ctaatgtata ttatcctaag atagaaagtt caattattat accaccatac tctgatactc
     5161 ttaattctaa agtgctatct agtaaaggtt atgatacatt taagaaattc ttagatcgtg
     5221 ctattaaaaa gaataagtta gaacgcttta tggatgagga ttttgaggac tttgccagtg
     5281 atattgctaa agaaacaggt tttgatactg ataaagtatc catagttttg aaagatttat
     5341 tgccttttga agattcttca ccatcaacaa caactagaaa tcaatataga gaagaagaat
     5401 atgaggcttt aattggaaat atagatgcta aatctaaaaa aactaatgac tttacaatag
     5461 aagaacaaaa tctatctgat tatgatattc caaacttcag taaaatagtg ttagtaaaaa
     5521 gacttagaga agtaagggca ttaactgggt ttagtagagt caatccacct gataataata
     5581 tatttggtga agaagatata gatgatagct gtaatgatac taaggttata agtttaagat
     5641 ctagagattg taaaaaatct ttttatcctg catctgaggt tagaggtgaa ggtattttca
     5701 tagagataaa taatgatatg gtaaaaagct ggatttcatc taatcctaaa atacaggata
     5761 atgcatctat tttaaattct agatataata aagatattaa tgttaatgac aaagcttata
     5821 gaaaaattac acctaaattc ttattactac atactttagc tcatttaatt ataagagaac
     5881 ttagttttgt ttgtggatat gctacctctt cacttaggga acgtatatat tgtaataatt
     5941 ccgaaaatga aaaagttatg tctggaattt tagtttacac tgcaagtggt gactctgaag
     6001 gtactttagg aggattagtt aggcaaggta tgcatgattc attaccatct atattaaata
     6061 gtgctcttaa tcgtgctctt tggtgttcta atgacccaat atgtattgat agtactggtc
     6121 aaggtagaaa ttctttaaat ctaggagcat gccattcatg tactttattg cctgaaacta
     6181 gctgtgagga gtataattta ttacttgata gaagcatgtt aattggtgat atggataata
     6241 gagatattgg gttctttaaa aatctaattt taatttagta ataactaatt ttttatttat
     6301 ataactaaag gttaaaagtt aattttatga cttctatcct ttagtttttt gtatttaaat
     6361 tattattttt tgcaaatcat tcttactaga aattacttat tataactaat tcccaataat
     6421 tatataattt tgtgctcata ctgaaatttt taaataaact attaatttat attatatatg
     6481 atttttcaaa ataaaaaaca tattaactat taaatcaata tgttttcgtg gattccaatg
     6541 gtggagaccg aggagaattt ataagtggtt atttgattgt tttgttggtt tttataagta
     6601 taggtatatg gctatttggt catgatgttg aagcaatttt tctggttatt ttttattgtc
     6661 tatttttgta gttggggtat tattggggta taattttcta ctttccaaaa ttattgatac
     6721 ttcaataata taaattaaat tcttagttaa gttttttaat gaataggtaa atattatttg
     6781 tactcataac aatatagttt acgcataatt ttatatatat tttctggtat atctgcaact
     6841 aacttagtgt aatttaaacg atattagaga tacttaaaaa acaaatatat gaaagattta
     6901 taactaactt agtgtaattt aattgattgc tctgcttctg tatttttaat taagttgttt
     6961 gaattattta taatataacc tccttaccac tttaattgtg agtcatctgg attatatatt
     7021 cctgtctctt tatttatgac aatttccttg ctattataat ttccctctag tatcttagta
     7081 aaattacttg aattcattat ccaatcaaaa tttgctttcc atcctgtatt attatttcca
     7141 ttcagaaaac ttgttttact aactatattt ataacctcta atacagtttc tttatcatat
     7201 tcttttattc ttgaatttat tctcctcttt cttgtatctg ttagtttctt gacctttgaa
     7261 agttcaatac aagttgagtt aaatttgctt atgatttcac tatatataat attattttca
     7321 ttctttatat tcttgattgt tgttgactga ttgttaatag tctgttgatt aggttgttga
     7381 ttttctatat tctcttgata aagctcccag ttttcaatag ttattactat atacttgttt
     7441 gttgtttcgt atgttagaaa accagcattt ttaaaaacca acaaacaact tctaaccatt
     7501 tgataactaa ctcctttacc acatctttca gctattttgc tatatgaaga aataaattgt
     7561 ccaaacttta tttttatctt tttattatct ataaaaacat gattttcttt atgactagcc
     7621 ataagtaata gagttattaa tattaccttt ctttctggtt taatatcttt ccaaattgat
     7681 ttatccaaca actccctata aagctttatc caccctttat tgctcaattg caaacaccta
     7741 ctttaagact ataacttgat accatttgca ttttcaataa accaatcatc taatttattt
     7801 tttattatgt atactcgatt atttatccat atacaaggga atcctttgtt tttagaatga
     7861 cataactgcc taaattttga ttctcctata ggataaagct tagatgcttc tttacatgtt
     7921 ataagctcag ctttatttct tttcgcattt tcaataattt tatatccttg ctttttttct
     7981 tgtgcttctt cttcattctt tacaataatg tttaaagtgg cttgtaatgt ctttaaaacc
     8041 tctaaattat tattctcttt catattttaa tcctccttac taaaaaataa ttcttctact
     8101 gttgtatcta aagctctaga tagtttaatc atcaaatcat tgcttggatt attagcctta
     8161 cctgtttcca agtttgataa ataatatctt gatataccaa ctctatctgc cagttcttgt
     8221 tgctttagac cttttttaat cctaaatatt tttaaattga ttcccatact ttttcctcct
     8281 tttttctagt ttacattcgt aaactttaca ttttaattat aatgtcgata tatgtgtact
     8341 gttaataaga atatattatt tttctgttct catttgtgta cttttgggat atactttata
     8401 cgaggtgata atattgcaaa ttggaaaaaa cttaaaaaag ataagaaaac aaaaagagct
     8461 aacgcagatt caacttgctg aaattagtgg catctcaaga aatgctctaa taaattatga
     8521 aaatgataaa agaataccta gtatagatac cttatcaaaa ctcgctaaag ctttgaaaat
     8581 tgaaaagact atcttttatt ttgatattga aaatgaagta atgactaatg aagatgtttt
     8641 atacctttta cagtctttat tcccagaaga atactattct tttgatgaat ttttaaatgc
     8701 tatttcaaat cttatggaag atgatattag aattacctca agtttattgc taggtgcctg
     8761 gtctgataaa gaccaatatt ataaacttgg tgaaaaatta gaactaaatg aaaatcaagt
     8821 ttataattgg atactttcag attgtatatc taatactgaa ttagattttt cattacatcc
     8881 tgatgacata aaagaaatta ttaaaaatga tgttcttaca aaacaatcaa ttgatgaatt
     8941 attagatatt ggtctatcag aagaaagtat agaaacaatc tatgattact ttagtgactc
     9001 aacagataat ggaagtaata ttcgactaaa aaaattaata tctcaaaaag aaaatattac
     9061 tattaaaaat aatgattact ttaaattatt acaagaaaat aacgaactaa aggaaaaaaa
     9121 tgaaaagctt gaaaatacac ttaaagaaat aaaaaaacta ctaactaaaa tataaaataa
     9181 tatcaatact atctaaagca atgataaaaa actatgaatg gagttacaat tatgaataaa
     9241 tcatatatgc taaaatacac atataaatcc cctactaatt tatttcatgg agataaaaaa
     9301 tatactctaa catacactaa gagagttatg agtaatttag tatatgatat gggataatct
     9361 agaaaataac tcctttacat gtttagaagt acaatcattg tttgatgata tatttaaggt
     9421 gaaattatga tgaataatac aattggagaa aagattaaac aatatagaaa atcgataaat
     9481 atgtcaaggg atgagctagc caacaagatt ggttgttcag ttcatgctat agctaaatat
     9541 gagcaaaatc aaagaatgcc tagtttagat atgataagaa aaatagctac tgctttatca
     9601 attgctataa ataaactcat tgttgaagaa aatgatttag atactttaac aaataataat
     9661 attaatttaa aagtatttga taaagagttg gatttagtaa ttcacatatt agaaaattat
     9721 ggttatgata taaaaacaga ctataaaaaa aagtttgaaa ttacagataa tgcagataca
     9781 ataaaaccaa ttaacctaaa cttctctgac ttcaaagact tctccaaaaa tctaaattgg
     9841 gtgataaatg gtcttgttga aaattttata caagaaaact ctaatggtct tattgaagat
     9901 gaggatgaag aatattagta ttgcacattt tcaaaatatg agtttaccta ttaactttta
     9961 tacattcata gttaataaat aaaaattaga tatgaaagga gctaaaatca tgaataaatc
    10021 atatatacca aaatacacac ataaattccc taataatata cctcatggag ataaaaaata
    10081 tactttaaca tacattagaa gaatgataag tgaatttgta tatgatatgg gtaacctaga
    10141 aaataacccc ttcacattcc cagaagtaca aacattactt gatggtataa ccgtaggcgg
    10201 acataaatta agtgaccaaa atcaaatact aaatataaaa tcatcatggg attatgtaat
    10261 aaaattaagt aacaaagaaa acttatcttt aaataaagat acaatttgct ctatacataa
    10321 aatagttgca aaagatgaag ctctaatcgt tggagatttc agaaatggta atataggtat
    10381 agctggtact actcaatatg aatgcataga agccactcta ttaaatgatt tatttgtaaa
    10441 tgacataaat atcataaata aaataactaa cccattagaa aaggctataa taataaattt
    10501 atggttgtcc tattgccaat tcttctatga tggcaacaaa agaactgcta gacttacctc
    10561 aaacctaata cttataaata atgatatagg agttctatca ataccagcta aacataaaca
    10621 agagtacaac acattaatgc taaacttcta tgaaacacta gaagctgatg aagttataaa
    10681 attcttatta gagaaatgta ttacattctt tgatggatat aactataaat catataagga
    10741 gctgtttaaa aatggcaact aaaagagcta atggagaagg ctctatcgga aaatataaag
    10801 atggttggcg ttcccgtata atgattggct ataatgaaga tggtaaacca ctgagaaaag
    10861 aattttatgg taagactcaa aaggaagtta aagataaatt agatgcttat aaaaaacaat
    10921 attatttaag ctctgatatt tcagatgata aaataacttt agagcattgg ttttacaatt
    10981 ggttatttga atatagaata aaagatttaa aacccaagtc atttgaaaga tatgaaagca
    11041 tctatcgtaa ttatattaaa gatactgatt taggcaatgt taaattaaag tacttaagag
    11101 tatctcacat acaaaaatat tacaatagat tgcttgattc caataaaagt atacctacta
    11161 taaggcaaat aaacactaaa ctgaaaacct gcttatcaga agctgaaaaa caaggtctta
    11221 tacaacgtaa ctattgtact atggtaaata taccagttga gaaaaaagaa aacaaacttg
    11281 aaatattgac tcttgaacaa caaaagagtt ttataaaagc tatagatggg cataaattag
    11341 aggtattatt tttacttgct cttggtactg gacttagatt aggagaatta ttaggactta
    11401 aatggtttga tattgatttt aaaaagtcta atttaacagt taaaagaact cttcaaagaa
    11461 catattttat agataaaact ggaaatagag agcttaaagt attagagcaa gggccaaaga
    11521 catcaaattc ttacagaact gtacctattc caaaagatgt tttaaataaa ttgaaagagc
    11581 ataaagtaaa tcaaaataca gatatattaa aggctggaga attatatata aataataatt
    11641 atgtattttg caatgaatta ggcatcccta tagatgataa aagaccttta aggaatttaa
    11701 agtctatatt aaatagctta aatatagagc ctataaagtt tcatggtctt aggaaaactt
    11761 atgctactag actttttgag aatgatgttc ctcctaaaac tgtacaagtt ttaatgggtc
    11821 attatgatat atcaataact ttgaatatat atactcaagt tatggaagat aagaaagttg
    11881 aagctgtaga aaagttggat aaaatatttt cattgtaaaa atcaaataca tataaatatt
    11941 gtgatttcat atagttaatt acaaaatcaa aataaggata caaattaatt ttatattata
    12001 ttgtatcctt atttcgtatc tagttttaat tatttatctt ggatagccct tatggtacat
    12061 taatccattt tctaacatta attcttgagc caagtatata tgacccttac agtgcatatc
    12121 tgatacaccc tcacttataa gtttatatgt ttcagaagta tataaataat ccatactttc
    12181 ttccaagctc ttacctgttt gtttggaaaa ttctaatatg atttttttat atagtatttg
    12241 taataatgtt ttatttgctt ccataatcat tatacctcct cttcatacct aaactttaaa
    12301 tatttatcta ttacattttg attggttata catatttgtt catttggttt atagtatttt
    12361 aatcttttta aagcttcatc tttttcaata agattatctt gatacagttc tattgtattg
    12421 taaatcttat catcagcaat accacctatt ataatgtcat atttcatata tatatcactc
    12481 atatttctac attctattat aaaatcaagc cattctaaat tataactatc gaagattttt
    12541 accttgcagt tttcttttaa tttatcaata tctaaagaat aactatttaa atatgctttt
    12601 ttacctcttt ttttaaaacg ctctgtccag ttaattgctt gtgtctttat actggttaca
    12661 tagaatcctt ttccaaaatc taaagaatct cttgaaaagg taatactagg cgtctttacc
    12721 acacaatatg aaccatgata tacaatcatt atatcacacc cttttcttta agtacttgca
    12781 ttaaatcttc cactatgtaa cctttacttt gagaatgtag tgcttcatag caaggtatta
    12841 tatagttatc taaaatatca gtatcatgtt taagaagttt ataaactcct tctgaatcta
    12901 tattcaattt ttctgctaaa gcttcaatgc aaaaaataga aaattctagt tttctttcat
    12961 ccattacaaa cctcaactcc tatcaagctt atataatata tatttttact aattaacaac
    13021 ttaaactgtc ttacatttat aaaaatatta tatcataaag tagatattaa aattctatgt
    13081 tacctattat cataataaac aaataaaata ataaacaaat aaaataataa acaaataaaa
    13141 actttttata cagcctaaat gcaccccaat atacccccaa aaaaacctca ttttctgtgg
    13201 ttggggtacg gttggtggat ttcttctcca aaataaaaaa tactagtcaa caaagactag
    13261 tatttccaag tatttcatca ttcttttttc aaaattggtg gagacgaggg gagtcgaacc
    13321 cctgtccgca aatcttttcc ttgctgcatc tacatgctta gccaacttca ctaatatctc
    13381 acctcacata gcgccaagtg gcaggctcta tgcttagcca gtcccaatta atctacttca
    13441 agtcgagaca cccctaaagt agttccctgc ataatgacgt ccagtcttag tcggcaggag
    13501 gactaagagg gacgagcagc aactaggctg ctatcgcgaa attatcgtct gcgtttattt
    13561 taaagttccc cagtttttac gtgacccaga gtaacacgac atgcagcctc aagttccaaa
    13621 cccacgtcga aacctttacg cccccttgag tagtttatag tccagtagac ttttctcaaa
    13681 ctttatcacc atttttatta aatttttaat gtatatataa tataatactt ttaagaataa
    13741 aaatcaatgt tatgttctag ataataaaga attttattct aaaaaataca gttttatcta
    13801 gtttaatact tacctgtcat ttctctttct atacgtcttt gtgcatcttt cttagccata
    13861 tcttgacgtt tatcatatag ttttttacct tttgctaagg caatctctac tttaacttta
    13921 ccgtttttta gatacaaact aagtggtata agtgaatacc cctttatagt agttgctcca
    13981 ataagctttc ttatttcatg cttatgaagt aatagttttc taactcttaa agaatcaaca
    14041 ttgaatatat ttccttgctc atatggactt atatgaactt gttttaaaaa tacttcgcta
    14101 ttatctacag atgcaaatcc atcacttagg ttaactttgc cttgtcttat tgattttact
    14161 tctgttcctt ttaattcaat tccacattca taagtttctt ctataaaata ttcatgtctt
    14221 gctcttttgt ttgttgctaa tattttttta ccagccatat aaatctcacc ttctttgcta
    14281 gaaatttgtt ccataaatgt tagaaattta ctaaatattt aatgttgcca aattatttga
    14341 ctaacttgta tgataacata tttaggcatt ttagtcaata tttcactgat atattaagtt
    14401 agttaggcta tatataatta agtaatataa atgtatgaaa atagctaaaa aactacttcg
    14461 aatatactta atactaaact aactctaacc agtaatacta atttataaat ttcaaaacaa
    14521 aaatattaca tattataaat cttctatctt ctatcttcta tcttctatct tctatcttct
    14581 atcttctatc ttctatcttc tatcttctat cttctatctt ctatcttcta aataatttta
    14641 acatgcccat aaaatataat acccacaaat gataaataat actcaaaatg tctattgaaa
    14701 taactataca tccttaaata acttaactta aatctaaaca tttcaaaaac ttcttaaaaa
    14761 tatgtaaaaa tttgatgata aagtagcgat tttttcaaaa aaaatgtaat atagagttat
    14821 atcttataat attttagaga taaattttat catagtgggg agaaatttgt atggatttaa
    14881 gcaaaaaagt aaaaatttta gaaaatgcat cagatggcaa aagcgaattt gagatattag
    14941 aatttgaaaa acttgaggga gcttctagcc cattaatggc aatgggatta tattttgcta
    15001 gagaagctgg tttgaaagct agacaaatta agataaaatt aaataattca tcaataaaaa
    15061 cagaagctgg tgcattatac tattataaag gaaatatatc ttctgaaact aagattggtg
    15121 gtgttggagg tctttttaaa aaaggaatat caggagcact tactaatgaa tcaatgatga
    15181 aacctgtata caaaggtact ggagaagttt atttggaacc ttcttttaga cattatttat
    15241 ttatgagtct tgataatgat tctataattg ttgataaagg attattctat tgttgctctg
    15301 atacaataga agtaaaagca tttacacaaa agaacttctc atctgcttta cttggtggtg
    15361 aaggcttctt ccaaatagaa ttaagtggta ctggtgtaat cgttctggaa tgtgttgtac
    15421 ctcaaagtga aattgtagag tatgaattga aaaatggtga agaattaaag gtagatggta
    15481 actttgcaat agcaagaaca tctggtgtta atttttctgt tacaaaatca gataaatctt
    15541 taattggttc tgctttaaat ggtgaaggtt tcttaaatac atttactggt agtggtacag
    15601 tttggctagc tccaactcaa gtaatgtatg aaaaactaag ttttggagca atgcctacta
    15661 atgataatat gaataataaa gtttctaagc aaaaatagtt gttttataaa tatttattaa
    15721 atcattattg attttataaa gtcactataa attttattag attattatta taaaatcaga
    15781 gtgtggaaat atttatgtgt attctttaaa taaaaatttc cacattctgt aaagttatta
    15841 tacttgatat ataaaattca atttaggaac atagtctaac atttataaac atattaaggt
    15901 aatgatttct cttggtagtt attctcatat cttattcttt gattatattt atcttattcc
    15961 tagcccttat gtcatattat atttcttatc ttattattta taattagcat taacttatct
    16021 aaaatcttgt atttttttaa tagtaaagtt tacatctata ttaatttcat aaaacaatca
    16081 aggtagtcaa gagattcaac tttatttgat tt
//
LOCUS       DAEMIK010000097        16092 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEMIK010000097 DAEMIK010000000
VERSION     DAEMIK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.097"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..764
                     /locus_tag="KPK87_002558"
     CDS             114..764
                     /locus_tag="KPK87_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG2538699.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(904..2280)
                     /locus_tag="KPK87_002559"
     CDS             complement(904..2280)
                     /locus_tag="KPK87_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2538700.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2296..4209)
                     /locus_tag="KPK87_002560"
     CDS             complement(2296..4209)
                     /locus_tag="KPK87_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2538701.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4345..4977
                     /locus_tag="KPK87_002561"
     CDS             4345..4977
                     /locus_tag="KPK87_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG2538702.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5116..5655
                     /locus_tag="KPK87_002562"
     CDS             5116..5655
                     /locus_tag="KPK87_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG2538703.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5831..6001)
                     /locus_tag="KPK87_002563"
     CDS             complement(5831..6001)
                     /locus_tag="KPK87_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2538704.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6286..7353
                     /locus_tag="KPK87_002564"
     CDS             6286..7353
                     /locus_tag="KPK87_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG2538705.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7523..9424
                     /gene="cooS"
                     /locus_tag="KPK87_002565"
     CDS             7523..9424
                     /gene="cooS"
                     /locus_tag="KPK87_002565"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG2538706.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9417..9890
                     /locus_tag="KPK87_002566"
     CDS             9417..9890
                     /locus_tag="KPK87_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2538707.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9865..11091
                     /locus_tag="KPK87_002567"
     CDS             9865..11091
                     /locus_tag="KPK87_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2538708.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(11230..12417)
                     /locus_tag="KPK87_002568"
     CDS             complement(11230..12417)
                     /locus_tag="KPK87_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2538709.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNTRIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12690..13514
                     /locus_tag="KPK87_002569"
     CDS             12690..13514
                     /locus_tag="KPK87_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538710.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVYKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            13643..14908
                     /gene="gluD"
                     /locus_tag="KPK87_002570"
     CDS             13643..14908
                     /gene="gluD"
                     /locus_tag="KPK87_002570"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG2538711.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15121..15234
                     /locus_tag="KPK87_002571"
     CDS             15121..15234
                     /locus_tag="KPK87_002571"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538712.1"
                     /translation="MHFKSKDFHLKYLVLFLKIVYWFNPFIYNGKIYGFGL"
     gene            15218..15709
                     /locus_tag="KPK87_002572"
     CDS             15218..15709
                     /locus_tag="KPK87_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG2538713.1"
                     /translation="MDLDCEFYCDERVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFV
                     AGLNKQSDIRKRVINMFSEKYKNGILMALIISLFSSITFFKFDSTSIIEENDFLERSR
                     YTFEGRQEDVFVTIDFIYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMD
                     LKK"
BASE COUNT         6159 a         1925 c         2642 g         5366 t
ORIGIN      
        1 atgaatttgt caagtttaat ttagttgtaa ttttatcttt tattaaaaat aatataaatt
       61 aaaacatata aataaaatat tttatacaat atattgtaaa ggaggtaact ttgatgaaag
      121 cagaaaaaac aagtatatat ccaattatta aaaataattt aaacaaaata tcaattgttg
      181 aatataaaaa gggtcaaaga tttgtagcat cagataaaga cttacaagaa gtgttcttta
      241 taatagaggg agttgcatta gtggagtgta ctactagagg aggaaataaa tttcttgttg
      301 acattgtacc tgaaaatgaa tttataggca aaattagtta catatatgaa cataatttga
      361 aatgtgatat atttgcaaag actaatataa aattatttcg atttgaaaaa aatgtatttg
      421 aagagtttta tctcaaacca gattttattg cactatttca cagaaaatgt acaagaagaa
      481 tatatgaatt atataaaacc agaatggtaa gagaactatt ttcttgcaca gaagttattg
      541 cctattgtat atcaaataaa caagaaaata atgtctgcaa tataaaagct attcgtaatt
      601 tcagtgaaat ctattcaatt agtagaaaaa gtagatacca ttctctgcta aagttaactg
      661 aaaaggagat agtaaaaaaa atgggtaata catatgaaat tttaaactat gatgaattac
      721 ataaattggc atttgaagta aaagaatttt tggaagatga gtaaaaattg ttacttaatc
      781 aaaataaagt ataataattt ctgtaaaaaa tagaaatagt ctcactgaaa atcaaaaata
      841 ctttagttat tttcaatgag actgtttaga aatattgtat acttataatg tatagtataa
      901 ttattaatca atttccttta ctatattaat agcccatttt ttagataatg ctctaggaac
      961 accaaatata aacttaacca cttcttcact ataagtgagc aaaacgagta caaatatagg
     1021 tgcaccttta aatgcagcta taaaagttag aggaatagaa gctaaccaca tacatcctaa
     1081 ttctaaaaat agagcatatt tagtatctcc tccacttctt aaaataccaa ttataaccaa
     1141 tgtattaaaa gatttaagag ccattagtat tcccattata aagaatatct tagttatatc
     1201 agaagctaag ctatcagaaa cactaaacat ttttaataat aatggtatat ttaaaattaa
     1261 aataattcca agtattaaac cagctgaaaa tacaagtata gaaaactttt tagcatattc
     1321 aatggctctt ttaatatggt cagctccaag ttcatttcca agcataatag atgcaccaga
     1381 tgcaatacat acagctgtca ttatgaagaa attacctgta ctggttgcta tctggctagc
     1441 tgctatagca gaagtaccag ctcttgcata tgcaacagaa tacaggacag ttccaattgc
     1501 ccatacagag tcatttacaa atataggaaa gctttttgat gaaaaagatt ttataaaaat
     1561 tttatcaata gcttttagat tttttagacc aaactttaat atatagtctt ttttataaag
     1621 gtaaacataa gtaatcatta aaattaattc acatattcta gcaataacag tagctagagc
     1681 agcacctttt acacccaatg caggaagacc gaagttacca aatataaatc cataatttaa
     1741 aataacattt gttacaagtg caaatgcact acaaatcata cctaacttag gatttctaac
     1801 acctcttgaa ccagtactaa atacagtact tacagcaatt aatggataac taaatactgc
     1861 aatactaaag taatctatac aaagttttac cacctcagaa tcatacgaaa atatgtgtat
     1921 aattggcttt ggtgttagta aagcaggaat aacaaataca aaacttaaaa caattgccaa
     1981 tacacaagta agacctgtga cttttcttat attactcaca tccttctttc cataaaactg
     2041 tgaaataaaa actccagcac ctcctgtgat accagataag gacatattaa aaagaaagaa
     2101 aaattggttt gcaactccaa ctgaagctac agatgtttcc cccaaactac ttatcataat
     2161 tgtatctatt acatttacag aagtagaaat taagttctgt atgataatag gtatacataa
     2221 agatagaagt attttataaa atcttttatt ttcgtctttt aaagaaaata gttcttgcaa
     2281 ataatcctct cctttttaca taaattcttc ccactcatca tatagagagg caaggttatt
     2341 ttctaaatta attttttctt ggctaacttc tttagcttta tctggattag agtatacttc
     2401 ttcttgacat aaaagtatat ctaatttttc aatttcaatc tctaactttt caatttcttt
     2461 ttctatattt tgtcttttta ttctattttt cttttctatt tctctttgtt ctctctcttt
     2521 tcttttttca tcttttattt gagttctagt tttttcttct ttttcttcta taatactcat
     2581 ctcttggatt tgcctttttt tatctatata ataatcataa tttcccagat attcagtaat
     2641 tccattttca tctaaaacta aaattttatc aacaacagta tttagaaagt atctatcatg
     2701 tgatattgta aatatagtcc catcataatt agttaaggcc tcttcaagga cttccttaga
     2761 atcaatatct aggtgattag taggctcatc taataacaat aagtttgcat ttgatagtat
     2821 aagtttaagt atggcaactc tagctctttc tccaccactc aatgtagaaa tcttcttaaa
     2881 aacttcttca tctacaaata aaaatgcccc taacatattt ctcaaagtag tttgagtaag
     2941 atgagtatta ttttcccaaa tctcatcaat tattgtgtta tctaaattaa gagttttttg
     3001 ttcctgatgg aagtaggaaa catgtacatt agttccaaat ttaatattgc cactaagagg
     3061 ttgtagttca ttagttatta ttttaaataa tgtagatttg ccaacaccat ttgcacctat
     3121 aagtgcaact ttttcccctc tatatatatc taaatccaag tctttaaaaa gtattctttc
     3181 tccataaccc atagatacat ctctaatttg aagaacatca ttaccactct ggacagaagg
     3241 cgtaaattgt atacgagctt ttttcctata agcttctggt ttatctaaaa catctacttt
     3301 gtctaatgct ttttctttac ttcttgcacg ctttaggtgt ttttctctac cataagcttt
     3361 tagtctttca attgattctt cttgcttttt tatttctttt tgttggtctt caaatttctt
     3421 aagctctagt tctttttcta ttttagataa ctcaacaaat ttagagtagt taccattata
     3481 tactttaagc tttttattgt gaacttcaaa aattctattt acggattggt ctaagaaata
     3541 tctatcatga gatattaaca tgacagtccc tttatattgt tttaaaaata cttctaacca
     3601 ttctattgct tcagagtcta agtggttagt aggttcatca agtaacaata gagttggttt
     3661 cttaagaagt aattttccta aaagaactct agttttttca ccaccagaaa gtatgcttat
     3721 tggcttatcc atatcattgt cactaaatcc taatcccttt agaactcctt tggcttcaga
     3781 tttatatcca tatccattta aatctgaaaa taattctagt ttatgtgaat attcatccat
     3841 gattttttct ataattggag agtttgtttt tgtactttct tcagatattt taacctccaa
     3901 ttctcttaaa taggattcca tttctatcaa atttttaaaa acttctagta cttcttccaa
     3961 aatagtatta tccgaataaa aattggtatt ttgttctaaa tatccgattt cacaatcttt
     4021 agaagtgtaa atatctccac tatcatatcc ataaattcct gaaattattt taaataaagt
     4081 agtttttcca gtaccattaa caccgattat accaatttta tcgccttcat ttactgtaaa
     4141 actaatattt tctaatatag aatctatacc aaaactttta tttaagttat tacatgacaa
     4201 aacaatcata aatttctacc tccttgaact atctaaaatt ataatgtttt gatataatat
     4261 aatcaaggtt atatgtatta gttttgaaaa agaaatattt tattgtgaat caaacaaaaa
     4321 agaggacact tttaggaggt gaagatgttg ggaaataaaa atatatcaat ggcagttata
     4381 agaaggctcc caaaatatca tagatatctt ggagacttat tagataggga tatacaaaga
     4441 atatcttcta aagaattgag tgatataata gggtttaccg cttctcaaat aagacaagat
     4501 ttaaacaact ttggtggatt tggacaacaa ggatatggtt ataatgtaga agctcttcat
     4561 actgagatag gtaaaattct tgggttggat cgaccataca acgcagttct tgtaggagca
     4621 ggtaacttag gacaagctat agccaattat gcaggattta gaaaagctgg attcgagata
     4681 aaagctttat ttgatgcaaa tcctagaatg ataggtttaa agataagaga gtttgaagta
     4741 ttagattcag atactttaga agactttata aaaaacaata atatagatat tgctgtatta
     4801 tgtataccta aaaatggagc acaagaagtt attaatagag ttgtaaaagc tggaatcaaa
     4861 ggtgtatgga attttgcacc tttagattta gaagttccga aaggtgttat agttgaaaat
     4921 gtaaacttaa cagaaagttt atttacctta tcgtatttaa tgaaagaagg aaagtagaca
     4981 acttaaacat attaataatg agcagttgta aagagtattg agaaagtcag aagatttaat
     5041 attaaatcct ctggcttttt gaaatattag gataaaaatt taaataatgt atggaaataa
     5101 acaatattaa ggagagtggt ttttgtggag gaattatgga aaaaggcaat tgaatttcat
     5161 ggtcatgaat gtccagggtt agctatagga tttaaagcag cacttgcagc aaaaaaatat
     5221 ttaggatgta aacagtcagg agatgaggaa attgtatgta tttctgaaaa tgatgcttgt
     5281 ggaatagatg gtatacaagt agtgctaagt tgcacaatgg gtaagggaaa tttactactt
     5341 aggatgactg gaaaatcagt atatcatttc tttaatagag aaaatggaaa atctataagg
     5401 atatatgtaa agtcaaaacc atttgagatg gatagagaag gatacaaaag atatttatta
     5461 gaaagtgatt ttgaagaaat atttgagatt acagatacta agttaagact tcctgaaaaa
     5521 gcaagaatat ttaaatccct taaatgtgaa gtgtgtggtg aatttgcatc tgagcaatat
     5581 ataaggattg aagaaggaaa gaaagtatgt cttgattgtt ttgataaata tgaaagattt
     5641 tatgaagagt attaaaatta taaattagat taaagttaaa agtattagat atgtgctaac
     5701 agtattttaa gatgaattag gtagcaacat aataaagtat ataaataata ttaattgaat
     5761 catacataat taaaaagagg ctgattagta tttataaaat aaaatcagtc tctttatgaa
     5821 taacgaatta ttattctggt tgaggtgcat ctacaggaca agctcctgca caagaaccac
     5881 agtctataca agaaccagca tctataacat atgcgtcgtc tccagctgat atacagctaa
     5941 cagggcattc agcctcacaa gcaccacagc ttatacaagc gtcagtaatt ttatatgcca
     6001 ttttgaaatc ctccctttta tatgtattgt aattcaataa cattataaca aatattattg
     6061 aatataacaa ttaaattaat tctacaacaa aaactttatt atgatattct tgttttaaag
     6121 ttttacaata aagtttaatc tgcttatatt tttatttatt ttaaagaagt ttattttaaa
     6181 aataaaatta aatttaaaaa gttatgtatt gattatattt aaaaatgtta tactctaaaa
     6241 aagtatattt tatttaaaaa tttattaata caaaggagat ttactatgaa aaataaatta
     6301 gtttacatat gtattatgag tctattgcta tttgtagtag gatgttctaa taatccaaat
     6361 aaggaaagta ccattaaaaa agactctaag aataaccaaa gccaagttaa agaagaaaaa
     6421 agagaacctg tgaaaaaaga tataactata agtttcgctg gtgatgtaac tatggggaac
     6481 tataaaggtt catcatatta tggcagtttt gaccatgaat ttaaaagaca aggagaagat
     6541 tacaattatt ttcttaaaaa tgtaaaacct gtatttgaaa aagatgactt aacagtggtt
     6601 aatttagaag gtccacttac tacagcgtca aatgcaaaag aaaaaaaatt tgcatttaaa
     6661 ggaaagcctg gatatgtaaa tattttaaaa tctggaagtg tagaggcagt aagtattgca
     6721 aataatcatt cagaagatta ttttgaagaa ggaatgaaag atacacagtt tatattggac
     6781 gagaataaaa taaattattt tggattaggg aaagatgctg ttgtagatgt caaaggaatt
     6841 aaagtgggtc tattagggta taatggatta tcaactgaat ataatgaaaa aaatttaaaa
     6901 caaatggaag atgatattaa gtccttaaaa aagaaatcgg atgttgttgt agtgtacttt
     6961 cattggggga tagaattaga ttattatcca gataaaaaac aaaaagattt tgctcattat
     7021 gctatagaca agggagcaga tttagttatg gggtctcacc cacatgtgat tcaaggaata
     7081 gaaaagtata aaaataagta tatagcttat agtctaggga atttttgctt tggaggaaat
     7141 aagaatcctt ctgatacaga tagttatata tatcaacaaa cctttacttt ttcagataat
     7201 aaattaactt caattaaaga gccaaatata ataccaactt caataacatc tagtaactcc
     7261 agaaataatt atcaaccaaa aatattggat ggaagtgaga aggatagagt tttaaataaa
     7321 cttgaaaaaa taagcaaaga tttaaataaa taaaatgtaa aaaaaaataa atatatatat
     7381 ttattttaat aatttatatt ttgtttaggg tatggtaaat cgtggtatta aataagtaga
     7441 ttttaatagt taaattatag tcaattataa taaagctatt taatctactt ttttaatata
     7501 aaaatataag ggggaatttg taatggcttg taacacttgt aaaatgtgcg aaagtgcaga
     7561 caagaaatta gaaagttttg tagcttcaaa ggatgttgaa acagcatttc atagaacaga
     7621 agatcagaaa gttaagtgtg gttttggact tcagggggtt tgttgtagac tctgttcaaa
     7681 tggaccatgt agagtaactc ctaaatcacc aagaggtata tgtggagcag atgcagacac
     7741 tatagtagca agaaactttt taagagcagt agcatcagga gcagcttgtt atttacatgt
     7801 ggtagaaaat acagctaaga atttaaaaaa tgttggaata actaaaggtg ttgttaaagg
     7861 agaaaaaaca ttaaatcaat tagcagagat gtttggaata gaaagtgatg aaaagtatga
     7921 taaatgtata aaggttgcag acaaagttat caacgatttg tataaatcaa gagatgataa
     7981 aatggaatta gtagaaaaaa tagcttatgc acctagagtg aaaaagtgga aagagttagg
     8041 tataatgcct ggtggtgcaa agtcagaagt ttttgatgca atagtaaaat catcaacaaa
     8101 tttaaatagt gacccagtag acatgctagt taattgtttg aacttaggta tatcaacagg
     8161 attatatggt cttacactaa ctaatctttt aaatgatgtt atgttgggag aaccagttat
     8221 aagaatggct cctgtaggat ttaatgtaat agacccagac tacataaata taatgataac
     8281 aggccatcaa cattcaacat ttgcaaattt ccaagataga ttaaaagatg aagatgttat
     8341 aaaattagct caatctgttg gtgcaaaagg ctttaagcta gtaggatgta catgtgttgg
     8401 tcaagattta caattaagag gagaacatta tcaagaagta tttgctggac atgcaggtaa
     8461 caattttaca agtgaggctg ttttatcaac tggagcaata gatatagttc tttcagaatt
     8521 taactgtact ataccagggc ttgaaccaat agcagataaa tataaagtaa aaatggtttg
     8581 tttagatgat gttgctaaaa aagcgaatgc agaatacata ggtttagaca gaagtaaatt
     8641 agatgaactt agtaacactt taatagaaaa agcattggag tcttataagg agagaagagg
     8701 aagtattgaa atagatatac caaaagacca tggatttgaa caatctttaa ctggtgtaag
     8761 tgaaaagaac ctgaaagcgt ttttaggaga ttcatggaaa cctcttatta atttaattgc
     8821 agaaggaaaa attaaaggtg ttgcagctgt tgtaggatgt tcaaatatga cagcaggagg
     8881 acatgatgta aatactgttg aacttactaa agaattgatt aaaaaagata taatagtact
     8941 atctgcagga tgttctactg gaggattaga aaatgttggt cttatgtcac ctggagcaga
     9001 agaattagca ggagaaaact taaaagaagt atgtaagact ttaggaatac caccagtatt
     9061 aaactttgga ccatgtctag ctataggaag actagaaata gtagcaacag aattagcagc
     9121 tgaacttgga attgacctac cacaattacc attagtgctt tcagctccac aatggttgga
     9181 agaacaagca ttagctgatg gagcgtttgg tcttgccctt ggcttacctc ttcatttagc
     9241 tttacctcca tttataacag gaggaaaatt ggtgacagaa gtattaacag aaaaattaaa
     9301 agacttaaca ggaggacatg ttatagtgaa tccagaccct aaatcttcag ctaatcaatt
     9361 agaagaaata ataatagata gaagaaaaaa tttagggtta aaggatgtgg aatgcaatgc
     9421 atagaatttt tataaataaa gatttatgta caggatgtaa gagctgtgta ctagcatgta
     9481 tgctaaaaca caataaagat tatgacatgt acactcttga tttagaaaat atagataatg
     9541 atagtagagg gcatatagaa ttagatagta gacataataa tccagttcca attctttgta
     9601 gacactgtga tgaacctgaa tgtgtattag catgtatgag tggtgcaatg cataaagata
     9661 gtgaaagtgg aatcgtttca tatgatgaag agaaatgtgg ttcatgttat atgtgtgtaa
     9721 tgtcttgtcc atatggatta ttaaagccag atgatagaag caagcagaac atacttaaat
     9781 gtgatttatg taaagatgag gagtatccta gatgtgtagc taattgccca agtggagcaa
     9841 ttgaattaca gaaggaggaa aatgatgaat tatgtagtgt taggggctag tgcagctggt
     9901 ataaatgcag taaaaacatt aagagaatta gataaagatt caaatatagt agtaatttct
     9961 aaagatgaaa acgtatattc aagatgtatg ttgcatcacg ttatatctga acacagaact
    10021 ttaaaacaaa taaattttgt tgatgaagat tttatggagc aaaataatgt aaaatggatt
    10081 gcaggtaaga ctgttaaagg tatagacata aataaaaagg tagttcagac agaagacatt
    10141 actgtaaatt atgataaact tttaatagct acaggtgcat cttctgctat tcctcctata
    10201 aaaaatttaa gagatggaaa ttttgtatat tctgtgagaa acatagatga tatttataaa
    10261 ataaaagaaa aagcacaaaa ttctaaaaat gtagttataa taggtgctgg tttagttggt
    10321 atagatgcat tagtgggatt atttaaatat gagcagttaa atatttctgt agcttttatg
    10381 gaaaagtaca ttttagatag acaattggat gaatatacag catcagtgta tgaaaataag
    10441 ttcaaagaaa aaggtgtaaa attttaccca agtgcatcaa tacaagaaat tgtattggat
    10501 aacagtaaaa atgttacagg tgttgcattt tcaaatggtg aagttttaga tgcagacatg
    10561 gtaattgtag ctacaggggt taaaccaaat gctgactttt tagatggaac aggaatagaa
    10621 tatgatagag gtataataat agatgatatg tgccaaacaa cacaaaaaga catatatgca
    10681 gcaggagatg ttgtaggtaa aaatgctatt tggcctctgg cagtaaagca aggaattgta
    10741 gctgcctata atatggttgg aaaagataag aagatagaag atgaatttgc ttttaaaaat
    10801 agtatgaatt tcatggatat accaactatt tctataggaa tgaatacacc tgtagatgat
    10861 agctataaag tattaaccag acatggaatt aatgattata aaaagtttgt ttttaaagat
    10921 aatgtaatct atggagcagt tatacaagga gatatttcat atgtaggtgt attaacttat
    10981 ttaataaaaa ataaagttga aatatatgat ttagaaaata ggatatttga tatatgttat
    11041 gcagactttt ttaatataaa agaaaatgga gaattttgtt atagtgtata gtatatacgt
    11101 ggagtaaggt ttataaatac agtaaatcaa taaatagagt tattctaaaa taagaaaaat
    11161 gacaaatctt atttagaaat agctctattt tttgtaatta aatttaaatt atattcaatt
    11221 tgtaaaatat tattttgaac aattatacat atactctcta gttagaggta aatcattttt
    11281 tataccttta gtgaaaagta tttgatgtat atctataata ccagtgttga aagcagcagc
    11341 acaagaacaa aggtataaat cccacattct tataaactta tcatcaaaca tttctttaat
    11401 ctttggaaga ttctcagaga aatttttttg ccagtatagt aaggttttta cataatgtaa
    11461 tcttagactt tctacgtcta tagtatgata tttgtaatca ctacaaatat gtatcatttc
    11521 tcttaggcta ggaataactc ctcctgggaa aatatatttt tttatccaag ggtctccata
    11581 agattcctgc aatccactta tatagtgaag taaaaacaat ccaccatctt ttaaaacagc
    11641 atttacgttt ttaatgaaaa gttcatagtt ttctctacca acatgttcaa gcatcccaac
    11701 gcttacaact ctatcaaaca taaggtctga tttttttaat tctctatagt ccataagtct
    11761 tacctcaaga tagtcttgaa ggttctcttc tttaattctt gtgttaaatt ccttaaattg
    11821 ctcttcgcta agtgtaattc caacgccgtg aattttatat ttttttgcag cttctattag
    11881 taaaaatccc caaccacaac caatatcgag aaggctcata ccttcctcta aatttagttt
    11941 agttaatatg tggtgaactt tattgacttg agcatcataa agagtatcat tatcatttct
    12001 aaagtatgca caagaataac taagtgtctt atctaaccat aagctataaa aatcgttgcc
    12061 aatgtcatag tgggcatgta tttctttctt ttgatgtctc attgaatttg atgagtgcaa
    12121 tagatgtttt acagctgaat tatcaatatt gaatttgtct atttgtttta aaagtagatt
    12181 taaagttaag aataaatctc cctcaacttc aatgtctcta tttatgtagg cttctccaag
    12241 tgctaaagat gtacttttta atagttcaga cttgctaata tcattattta ctataacttt
    12301 gaactgaggg gagccctctc caattagaaa ttcttcctca tctttaagcc taactagaaa
    12361 tggtgtttca ccaaatctct ctaaatagtc ttttaaaaaa ttttttgtaa tattcatatt
    12421 accagctcct tctaatgtaa atccagtaag tttccctgct taaaatatat tatagtcctt
    12481 tttttaaaaa agtaaaattt tttcaataaa ttttttattt aaaggaaatt tattttttat
    12541 aaagaaattt catgttgtag tatatttttt gcttataaat tttggtactg gatataatat
    12601 taaactgtta atgatataat aaatttaatc tataaagtaa ataaaatttt gtaataaata
    12661 gttaaagcta ttttataagg agatatatga tggaagtata tgctttggtt gggtcaagtg
    12721 gcacagggaa gagctttaag gctttagagt tcgcatatga aaatgatata gaatatataa
    12781 tcgatgatgg gattttaata tacaaaaaca aggttttagc tgggatatca gcaaaacaag
    12841 caaatactac tatagaagca gttaaaaggg ctatttttta taatctggag catagacaag
    12901 aagttagaga agcaattaaa aaagaaaata taagaaaaat tcttattatt gggacatcaa
    12961 aaaaaatggt taatcaaatt gtagagagat tgtcaattgg aagggtctat aagtttataa
    13021 atatacaaga tataagtact aaatatgaga tagaaatagc aaaacaagct agaaaagaag
    13081 gaaatcatgt aattcctgtt ccagcagttg agataaaaag tatggcaagt ggtcttagta
    13141 taaattcttt gaagagactt tttagaaaag gaaataatag gaatatggta gttttagaaa
    13201 aaacaattat aagaccaact tttagttata taggaaagtt ttatattagt gctgatgtaa
    13261 ttaaacagat tattgaatat gaagtataca actttggaag cattgataga ataaataaaa
    13321 tcaatataga gaattgcaat aattttatga atatttttgt aagtataaat atcaatgatt
    13381 tagaaactat aaaaaaaatt gaagacatac aacagacaat aaaaaagtct attgagaaaa
    13441 tgactttagt taatgtacaa aaaatagata ttaacattca taaaattaaa aaaataaact
    13501 tcctccaatg ttaaaaaata aactgagaaa atgatatact aatttgcaat aaagtgtgct
    13561 ataattaaac tgtaaattta ggaaatggtt ttttctaaat ggagtataca taattataac
    13621 gtatttataa gggggctttc aaatgtcagg aaaagatgta aatgtcttcg agatggcgca
    13681 atctcaagta aaaaatgcat gtgataaatt aggtatggaa ccagcagttt atgaattatt
    13741 aaaagaacct atgagagtta tagaggtttc aattccagta aaaatggatg atggttctat
    13801 aaaaactttt aaaggattta gatcacaaca taatgatgca gtagggccaa caaaaggtgg
    13861 aataagattc catcaaaatg tttcaagaga cgaagtaaaa gctttatcta tatggatgac
    13921 tttcaagtgt tctgtaacag gtataccata tggtggaggt aaaggtggaa taatagtaga
    13981 tccatcaact ttatctcaag gtgaattaga aagattaagt agaggataca tagatggaat
    14041 atataaatta ataggtgaga aagttgacgt tcctgcacca gacgtaaata ctaatggtca
    14101 aataatgtct tggatggttg atgagtacaa taaattaact ggacaaagtt ctataggtgt
    14161 tataactggt aagccagttg aatttggtgg ttctttagga agaacagctg ctactggatt
    14221 tggtgttgct gttactgcaa gagaagctgc tgctaaatta ggaatagata tgaaaaaagc
    14281 taaaatagct gttcaaggta taggaaacgt tggttcttat acagttctta actgtgaaaa
    14341 acttggtggt actgttgtag ctatggctga atggtgtaaa tcagaaggtt cttatgctat
    14401 atacaatgaa aatggtttag atggtcaagc tatgttagat tatatgaaag aacatggtaa
    14461 cttattaaac ttcccaggag ctaagagaat atctttagaa gagttctggg cttcagatgt
    14521 tgatatagta ataccagctg cattagaaaa ctctataact aaagaagttg ctgaatctat
    14581 aaaagctaaa ttagtttgtg aggctgctaa tggaccaact actccagagg ctgatgaagt
    14641 atttgctgaa agaggaatag ttcttactcc agatatatta actaatgctg gtggagttac
    14701 agtttcttac ttcgagtggg tacaaaactt atatggatac tactggtcag aagaagaagt
    14761 agagcaaaaa gaagaaatag ctatggttaa agcatttgaa tctatttgga aaatcaaaga
    14821 agagtacaat gttacaatga gagaagctgc ttacatgcat tcaattaaga aagttgctga
    14881 agctatgaaa ttaagaggat ggtactaaga tttactctta ataaataata ggatataatt
    14941 atttaagata taaaggggct gtacatagta cagcccttaa attatgtggc ttacaatgaa
    15001 gtaagctatt tttgttttaa cttataaaga acttgataaa ttatgtaatt tagaatgtat
    15061 tttttaccac cacatgatta caaattagag gagttagatt gaatttttag acatgaactt
    15121 atgcatttta aaagcaaaga ctttcattta aagtatctag tattattttt aaaaatagta
    15181 tattggttta atccatttat atataatgga aaaatatatg gatttggatt gtgaatttta
    15241 ttgtgatgag agagttttaa aaaattgtac tgtacaacaa aagcaagaat atgctctaac
    15301 tattgtaaat tcaatgaaaa gaggtacaaa tttttcaaat aaatttgtag ctggattaaa
    15361 taaacaatct gatattagaa aaagagtaat aaacatgttc agtgaaaaat ataaaaatgg
    15421 tatcttaatg gctttgatta taagcttgtt ttcttctatt acatttttta aattcgattc
    15481 aactagtatt attgaagaga atgatttctt agagaggtca agatatacgt ttgaaggaag
    15541 gcaagaagac gtgtttgtaa caatagattt tatatatgca gatgcaccta taaaacttag
    15601 aaaagagcat gaagaaaact gtagagtcaa tggaatagta ccaaaagatt cagattcaat
    15661 agaaattatt cctagatttt ataaagattg tatggattta aaaaaataag ttagacttaa
    15721 tattgatgtt gatattatga attttttata tttaagcaat tgaaaatttt attatagatt
    15781 caattttata cgtgtttaag tatgaaattt atagatagtt gaaataatta aatatgattg
    15841 atgcatagga atcattacta agctataatt aattataggg gagtttaaat aaattatgag
    15901 ttacacaatt ataaaatgat aaaaatagtt atctgttaca aaaaatgtat ctttttaatt
    15961 aataaagata atatgaaaac ttaaaaggca aataaactta aacataaaat actcttaaat
    16021 aagataggag aaaaatgtat gtctaacttt attctaaatg ggatttttag aacagttata
    16081 gtaagtagtc ta
//
LOCUS       DAEMIK010000098        16088 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEMIK010000098 DAEMIK010000000
VERSION     DAEMIK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.098"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..667
                     /locus_tag="KPK87_002573"
     CDS             <1..667
                     /locus_tag="KPK87_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2538714.1"
                     /translation="LVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESIVLDC
                     IILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKPYSLK
                     ELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEILLFLA
                     ENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWGKGYK
                     FVP"
     gene            739..2754
                     /locus_tag="KPK87_002574"
     CDS             739..2754
                     /locus_tag="KPK87_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2538715.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGQLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            2804..3484
                     /locus_tag="KPK87_002575"
     CDS             2804..3484
                     /locus_tag="KPK87_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2538716.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAVGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4000..4446
                     /locus_tag="KPK87_002576"
     CDS             4000..4446
                     /locus_tag="KPK87_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538717.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            4827..5744
                     /gene="pstC"
                     /locus_tag="KPK87_002577"
     CDS             4827..5744
                     /gene="pstC"
                     /locus_tag="KPK87_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG2538718.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYAIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5746..6570
                     /gene="pstA"
                     /locus_tag="KPK87_002578"
     CDS             5746..6570
                     /gene="pstA"
                     /locus_tag="KPK87_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG2538719.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6589..7353
                     /gene="pstB"
                     /locus_tag="KPK87_002579"
     CDS             6589..7353
                     /gene="pstB"
                     /locus_tag="KPK87_002579"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG2538720.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7381..8034
                     /gene="phoU"
                     /locus_tag="KPK87_002580"
     CDS             7381..8034
                     /gene="phoU"
                     /locus_tag="KPK87_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG2538721.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8192..9061
                     /locus_tag="KPK87_002581"
     CDS             8192..9061
                     /locus_tag="KPK87_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2538722.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKIIRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9148..10584
                     /locus_tag="KPK87_002582"
     CDS             9148..10584
                     /locus_tag="KPK87_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG2538723.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10632..11459
                     /locus_tag="KPK87_002583"
     CDS             10632..11459
                     /locus_tag="KPK87_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2538724.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    11754..12016
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12092..14758
                     /locus_tag="KPK87_002584"
     CDS             12092..14758
                     /locus_tag="KPK87_002584"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG2538725.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPEKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            15183..15893
                     /gene="rgaR"
                     /locus_tag="KPK87_002585"
     CDS             15183..15893
                     /gene="rgaR"
                     /locus_tag="KPK87_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG2538726.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         6370 a         1651 c         2916 g         5151 t
ORIGIN      
        1 actggttgta gatgatgatg ttgatatttt aaacataaat caaatttatt tttctaatat
       61 aggttttaat gtatatacat cagagactgc taaagatgct atggatatag tagagagtat
      121 agttttagac tgtataattt tggatatttc attgccagat atagatggat ataaaatgtg
      181 caatgatata aaagaaaagg ttaacatacc tataatattt ttatctaatt atgagcatga
      241 agaagaaaaa gttcggggat ttttagctgg gggggatgat tatataacaa aaccttatag
      301 tttaaaagaa cttgaactta gaatatatgc tcgtatgagg cagtacaaaa atattacatc
      361 agttcctaat gtgttaaagt tttcagcgtt agtaataaat gtaaattcaa gaaaagtaac
      421 ttttgatgga gaaagtatag atttaactac tatggaattt gaaatattat tgtttctagc
      481 tgaaaacaaa gaacaagtat tttcaaagat agagatatat aaccaagttt ggaaaatgcc
      541 tgatattgga gatcaacata cagtgcaggt tcatattgca cagatgagaa aaaaaattaa
      601 ttctttaagc agggagcatc aatacattca aaccgtatgg ggaaaaggat ataagtttgt
      661 accataagtg agatactgta attaataaat agttcttgtt ttatttaata cgactatgaa
      721 agagatgtgg ttgatgcaat gaaaaagtat aatatactaa aaggaactct cattgtattg
      781 atagtctctc tgttggcaat aacaggatta aatcttctgt ataaatatga taataaatat
      841 acatataagt ctattagagc aagtaatgga attttaacta tagataatga tactttcact
      901 aaagggcaac ttgtcttttt gatagatgac tgggagtttt ataatcataa aatatttaaa
      961 ccgaatgatt ttacagagca agatataaaa ccagaatatg tttttatagg tcagtatcca
     1021 gatttttcta tgagaaaagg gaatcagtca ccatatggga atgctactta ccgtatgaga
     1081 ataaaaaatg aaggcaagga taagctacta agtttagagc ttcctgagat tttttccgca
     1141 agtgaagtat ggataaatgg agaaatggtt tctaaattag gagatgtagg aacagaaaaa
     1201 tatagaccca aaattaaaaa ttcagtagta agttttattg caaagaaaaa tacagaaatt
     1261 ataatcaatg tatcgaattt ttcacattat tatagtggtt tgtattatcc gcctgcatta
     1321 ggagaaacaa aggacataag taatatgatg ttttatcgat tactttttta tagtataatt
     1381 tgcttcacaa cttttgcaat agctatattt tcattgtctg tatggtttct ttcaaacaaa
     1441 agtaaaattt acttatattt tggatgtatg tgtgttttct ttgccatcca tgtatcctat
     1501 ccctttgtac atttgttagg actgcctttg gtaagcttta catatgcggt tgaagatttg
     1561 tcatatttta tggtagtttt atgtattatt tgtataaatg gaagtatatc caaaatagaa
     1621 gaaaataaag tgtatagatt tttaatttta ccattaggaa ttacaatgag tatcatatct
     1681 atagtgatac caattattct tcttccatat gatatgtact ttgtcaacat atatgggaag
     1741 ttaatagaca tatataaata tattgtattt atatatatat taatgtcttc gctggtttca
     1801 atatataaaa agacatgctc tgagcatata agttcttata ttttagtttc agtaaacact
     1861 gtttttggaa ttagcatatt atttgatgca gttacgagta atagatttga gcctatattt
     1921 acagggtggc aaacagaata ttgtggattt gtaattgtta ttttattttc tgttatgatg
     1981 ataaacagga atagaataat aataaaagag aatgaaaaac ttacaaggaa tcttgaacat
     2041 gaagttgaag tgagaactaa tgaacttaca acattgttaa atgaacgtaa aagattcttg
     2101 gcagatgtag cacatgattt aaaggcacct gtttcggcta tacaagcatt tatagatttg
     2161 ataaaggttg gtaatattca tgtagatgaa gagacaagaa cctatcttta tgctataaat
     2221 caaaagtcaa atgaagtaca aaatagagta cgtagtttgc aagaatttac atcacaagat
     2281 aaaagcattg gtattagtga aaaaatatgt ctgaataatt ttattaaaga gatatatgaa
     2341 aataatttgc cagatacaca agcatgtggt gtaaactttg aaacttacgt attggagaaa
     2401 aaaatatatg caaagataaa taaagagatg cttacaagag catttgagaa tttaatttat
     2461 aatgctttga cctttactcc atttgatgga aagataattt tatcaatgga taaagaagat
     2521 ggatttgcaa ttattaaaat ttctgataat ggaaaaggta tacctccaga aaatatacca
     2581 aaggtatttg acagattctt tacagaaaga gacaataatg agccaaaagg gcaagggtta
     2641 gggctatata ttgtcaaatc cattataaga gagcatggtg gagaaatatt tgtagattca
     2701 gaactaggaa aaggaactgt ttttacaata aaattaagtc tatgtttttt ataataatag
     2761 cttatttaat ataaaagaat aatgatattt gggagtgatt ataatgcaaa ctaagatatt
     2821 gataatagat ggagacaagg ataattgcca gaagctaaaa ggatttctgg aggaaaaagg
     2881 aataagtata gatttagcat ataattgtga agaagctgtt ggaaaaatat ttagcaataa
     2941 atatgattta atatttttag aaataattct aagtgatggt gatggatgga ctctttgtaa
     3001 aaaaattaga aatgtaacta cttgtccaat tgtttatatg acttatataa atgaagacca
     3061 aagcatttta aatgctttaa attctggtgg agacgattac ttaataaagc ctctaaattt
     3121 agaaatatta tacgcaaaag taaaagctat tttaagaaga atgaactcat atgtaaataa
     3181 taatgaaaat aatacaaaat tagaggaata taatagaact agtggagtaa taaagttaga
     3241 agataaaata ataaaattga ctccaacaga aaataagttg ttaaactttt ttatagagaa
     3301 tcctgaaaag actttgacta ctaaagagat atatgaacat gtatggatga atgaatactt
     3361 agaggataac tatagtgtgg tagttgcagt gaatggatta agaaagaaaa tagagaaaga
     3421 ttataaaaat cctcagaaaa ttataacaat aagaggagca ggatattatt ttaataagga
     3481 ataataaata tattaatatc attaataatt gttataaatg tgaaatacta acgatatcta
     3541 aactaaatta ttataaaata gctgactgta atttaattga taaatcctat tcttctagac
     3601 tttgccagtt aaattacagt cagttttatt atataaaaaa atgcgtaatg gaactattat
     3661 tttatagatt atatttgaaa agatggataa attgtatatc taaatctata tattatttta
     3721 ttatttaata tatattaaat aataaaatag ttaaataata agattaagaa tttcttaata
     3781 aatccagaag atgctttcga ttatataata ttttatattt ttatattatt aattatatat
     3841 gttgcaattg ctgcacctac tttttctggt gagtatcaga cacaatcaga tagcatcctt
     3901 cgttgtacga aacatggaca tattcgtcta gcgtttacta aaatattagc tctattttct
     3961 atatttataa taatgtttgt tatatgtatt tctatccatt tggcaatttc tgatttagct
     4021 tttggtactg aatgtttgaa aacctcattt caaatgttgt tttctgtcat tagtctagta
     4081 aatataaatt tactacaagc acagattctt atagctattg gtggtctaat ttcaatactt
     4141 gcaatggtta gtttaacatt atttttatct gcaaaatgta aggattctct tacttctatg
     4201 ctgattgctt tttctatttg cttaattcct atgggaatag gtgttagttg gattggatac
     4261 ctattaccac cagggggaat tggttttaag attagtttat tatatcaaat gacatcattt
     4321 aatttcttac acttaggagg tatgagtttt tggacaccac acattatact gtttttctca
     4381 gtgatagaaa tacctatttt tatatttttg gctattcgta cctattgcaa acatcaagta
     4441 tcgtaacaat ttgatggaaa taatagtgat ataaactaga ggatacaata attatatatg
     4501 tagtataaaa gttaaattta gttaacaaat cttaatagca gatataggta ataaattata
     4561 cataaactgt attattatct atatctgcta ttttaatata gagaaacaac agtatattgt
     4621 aaagtaaatg tcaaatatag ttttaaaagt aaaattaatg ttaaaaagta aatataattt
     4681 tacacttgct taacttaatc ctaatataga tttaacttaa aaaaagtaga ataaaagtat
     4741 aggaaatttg atgaagacga attataaaaa attaaatttt gaatacagaa cgaaaatcga
     4801 taagtatatt ttaatgggag ggtattatga tagtaaaatc tagattagta ggtttagaaa
     4861 aaaatgacaa taatggcaat aagacaaaat acgcaattga aaaagtagca aaaaatattt
     4921 ttctacttag tgctttagta gcagttgcaa gtttactttt gataatagga ttcgtatttt
     4981 ataaaggatt aagaccattt atatttgaag gatattcatt tatagacttt ttaactggtg
     5041 cagattgggt tccttctgcc aataaatttg gaatatcatc tatgatagta gcatcaatat
     5101 ttgcaacact tggagcactt gttataggag tacctgttgg tatattaacc tctgtattta
     5161 tagcagaagt agcacctaaa agattagcaa aaataatgtc accagcagtt gaacttttag
     5221 caggtatacc ttcagtatta tatggtgtat ttggtcttgc agtgatagtt ccaactatac
     5281 aaagtatatt taatcttcca aaaggtcaaa gtttgttagc aatagtaata gtattagcag
     5341 taatgatgtt accaacaata atatctgtat cagaaacagc aataagagca gtaccaaaag
     5401 catacaaaga aggttcatta gcacttggtg catcaaaaat agaaacaata ttcaaagttg
     5461 tattacctgc tgcaaaatct ggagtattag ccgcagtagt gttaggagta ggaagagctt
     5521 taggtgaaac aatggcagta atacttgttg caggcaactc accagttatg ccaactgcaa
     5581 taacagatag tgtaagacca cttacaacta atatagcact tgaaatggga tacgcatttg
     5641 gtacacatca agaaatgcta tttgcaactg gagtagtatt gtttacattt atattaattt
     5701 taaacttagt tttaaataaa ctttcaaata aggcggtaaa ttaagatgag aaagttcaaa
     5761 gagaatctat tgaagtcatt agtatatcta tcagcactat ttacaatagt atcattagta
     5821 gttatagtgg gatttatatt tataaaggga attggcaaca tgaattttaa tttcttattt
     5881 agcaattatt cagcatcagg tgatggtggt atacttccaa tgataattac aacattatat
     5941 acagtagtag tttctatagt tgtagcaact cctataggaa tattggctgc tatatattta
     6001 caagaatatg ctaaaaaagg aaaagcagtt acagctataa gatttgcaac tgaaagttta
     6061 tcagggatac catctatagt atatggatta tttggaggaa ttttctttgt agttacatta
     6121 aaaatgggat attccatagt tgcaggttca cttacagttg caataataat attaccagta
     6181 ataattcgta caacagaaga agctttaaaa acagttcctc aagcttatag agaatcatca
     6241 ttggcattag gagctacaaa atttcaaact ttatataagg tagtacttcc aagtgcaata
     6301 ccaggaattt tatctggagt aatcttatca gttggtcgta taattggaga atcagctgca
     6361 atattactta cagcaggtac tgtagccaag atgccaggtg gaatatttga tagtgcaaga
     6421 acattaactg tacactcata cttactgact aaagagagtg gagatatagc aacagcagcc
     6481 agtataggaa ttgttttaat agtaattgta ttagcactaa acatgctagc aagatttgtt
     6541 gctaagaaat taaataaagc taattattaa atttaagagg tgtgaataat ggaattaatc
     6601 gataagataa aaatgagtgt aaaagatttg gatttatttt atggtgataa gcaagcatta
     6661 aagaaaataa atatggatat aaaagagaat aaggttactg ctttaatagg tccatctgga
     6721 tgcggaaaat caacttttat aagaacgtta aatagaatga atgacttaat cgaagatgta
     6781 acaattaaag gaaacatttc tgttgatgga gaggacatat atacctcaga tgatgttata
     6841 aatcttcgta ctaaagtagg aatggtattt caaaagccaa atccatttcc tatgagtata
     6901 tatgataatg tggcatatgg tcctcgtaca catggactaa gagataagaa gcaattagat
     6961 aaaattgtag aagaaagctt aaaaggtgca gctatttggg atgaggttaa agacaggctt
     7021 aaatcatctg cactaggact ttctggtggt caacaacagc gtatatgtat agcaagagca
     7081 attgctatga gaccagaagt tattcttatg gatgaaccta catctgcttt agacccaatt
     7141 tcaacattaa aagtagagga acttatagag gatttaaaaa aagactacac aatagtcata
     7201 gttacacata atatgcaaca agctgctcgt atatcagatg aaacagcttt tttcttaaat
     7261 ggagaggtta tagaatttag cgatacaaaa actatgttta ccactcctgt agataaaaga
     7321 acagaagact atataacagg tagatttggt taaaatatat aactataaag gagttagaca
     7381 atggtcaata atagtttaga tataagtatt gatactttaa aagaacaaac agtgcttatg
     7441 atggaaaaat gtgaagaagc aatagaaaag tctgtgactt gtatgataga gaaggattta
     7501 gagggtgcaa gaaaagtagt agaagatgat gaagaaataa ataaattaag agaagaaata
     7561 agagataaga gtatagagct aatagtatta aagcaaccaa tggcaaggga cttaagattt
     7621 atttattcat taggtaatat atctatagag ctggaaagaa taggagatta tgcagcaaat
     7681 atagctagag aaatgttgaa gataggtgga gaggcttata taaaagagct aatagatatt
     7741 cctaagatgg caaaagaatg taagtctatg atgtctcaga ctagagaagc tttaatctta
     7801 tttgatgcaa gtatagctta tgatgttgga actaaagatg atattgtaga tgggttgtat
     7861 gaacaagtac aaattgattg tttaaaaata atgcatgaaa actcaaatac catagaccaa
     7921 ggggtaaaac ttctacttat aggaagatac cttgaaagaa ttggagacca tataacaaat
     7981 gtatgtgaga agataatttt tgcaattgag ggtaaaatgg ttgagatagg ataaaaaact
     8041 gctttaaagc agttttttta ttgtctaaat taagaaagaa ttgataaaaa aatatgatta
     8101 atgggtaaat ttaaaaaatt gtgatatact aggaacatat taaatatgag gatgcgtatt
     8161 ttatataagg tgctttttag gaggtggaac tttgaaactt caaaagatgg gtatgaggac
     8221 tataaaaaca ggaattagtg tcactttatg tatgattctt ggtgactatt tagtagagaa
     8281 tatgttttat tctctggtag catgtataat ttctgttcaa gatacagtaa aaggttcttt
     8341 aaagacggga atcaatagag tggaaggtac tattttaggt ggtataatag gattcatatt
     8401 tgccatcatt aaagctggag acccattttt atgtggtcta ggagtaatgt gctcaatata
     8461 tttatgcaat ctgtttaaaa taaagtcagt agtagttgcc tgtgtaacct ttctggctat
     8521 acatctaggt gtaggaaata gtgaccctgt gtattattct ttacataggg tgttagatac
     8581 atctgtgggt gttttgtttg gagtacttgt taattattat gttgctagac caaattatct
     8641 aaatagtgtt atagatgagt ttaaaaagat agaagaactt tcaatgttgc ttattgaaga
     8701 taaaattatt agaaaagaaa atttaaatgt agagaaattt gaagaagaag tagaaaaatt
     8761 agaagaaata tattcaaagc ttattgatga gttggattat agtttgagtg aaactaatat
     8821 agaaaaaatt gagaatgcct taaggatatg tagagaaata cattttcata tgcagtctat
     8881 agagttactt gaaaataaac tttatttgaa agagcataca tataaatacc ttaagaaaac
     8941 atataatgtg gataagctag actggaatat agatgaagaa aaaagccctg tattcaacta
     9001 tcatctaatg aaaataatga ttgaaataga gttgttgaga aaactaaata aatcatatta
     9061 ataatagtag ttaaataata aataaaaaat agtatctttg taaatattaa acatatataa
     9121 gtttaattta tggaggtact attttttatg aatactagaa atgacaattc taaaatacta
     9181 gaaatatata aaatgaagct ttttggcgaa cttgtaaaag cagtgtggaa tgatgacata
     9241 gaggatattg aaaacttacc taataaaatt ttaaatgaaa atttaaagga taatttaaat
     9301 aattatttta gtggaaaatt tgataaagat actatggtaa atttaattag ggtaactatg
     9361 ggattggatg tgacagaaga tacagataaa aatttaaaag agtcactaca tgaagctgta
     9421 aatttagatg aagtcaaaag accaataata tctgtaatat cagatgcttg caaatattgt
     9481 gatgaaaata ataaggaaga tgagtcatgt aagataagaa gtaagcatat taattgtaat
     9541 gaggaagatg tatgtagtgc ttgtggtgat tgtgtatcaa gatgtaagtt gggtgcaatt
     9601 tcagataaaa tagaatttat accaatgata aaattgttaa aagatataaa gagccctgta
     9661 tatgcaatag ttgcaccagc ttttgtagga cagtttggga aagatgtaag ccctggtaaa
     9721 ttaagaactg cattaaaaaa gataggattt gaagatatga tagaagttgc tttggcagca
     9781 gatatgctta ctgcaaaaga agcatatgat tattatgagc atatgaaaaa ggacgaagaa
     9841 ggatgcttta taactagttg ttgctgtcct atttgggtta gtttgataca aaatagtttt
     9901 cctcaaatat tggaaaatgt atcaccatcc gtttctccta tgattgcttg tgggagagct
     9961 gtaaaaattt taaatccaga tgctaaagtt gtattcatag gtccttgtat ggcaaaaaag
    10021 aaagaagcaa tgttagaaga tataaaagat gcagttgatt ttgtgcttac atttaaagag
    10081 ttgcaagaaa tatttgatgc attagagtta aatccagcaa taatggatga gagtaataga
    10141 gtagaggcgt ccctttcagg tagagtttat gctagaacag gaggtgtaag taaagcagtt
    10201 aagttatcag ttagaagaat tgacaaaaat ataaaatttg aagaaaaggc atttgaagga
    10261 acaaaagagt gcatggacgg tctaaagaaa gttctaaata aagaaataga agctacattt
    10321 atagaaggaa tggggtgtgt aggaggctgt gtaggaggtc cgaagcgtat tttaagtgtg
    10381 gaagaaggta ctaagaatgt tgatgaatat agtgaaagta cgaatatgga aactccattt
    10441 gataatttaa atgtattaca atttttaact tctttgggaa tcaaaagagt agaatcttta
    10501 ggagaaaaag aagaagaaaa ggttaaaaaa atatttagta gagatataaa aagtaataag
    10561 aaagagaata aatataaaaa ataacttttt aagtcttaaa cttatgcttt tataaaaggg
    10621 agatatgggg gatgaaaatt aaagataata aggctgtttt tcatgtattt ataaggatat
    10681 ttattatagc tgttttaatt ttaactggaa cattattgga ggaaaaaatt aataatgttc
    10741 cagttgaaaa tataagttca aaaggtgaaa atacactgga actgctttct caaagcaata
    10801 ctttaggaga aatcatatac aaagcatctc aagaagaaac aaaaggtcca ggggctactt
    10861 ctgaaaaagt agcatacata acaatagatg atggtccatc aaaatataca aatgcactac
    10921 ttgatatttt aaaagaaaat gatgtcaaag cgacattttt catgcttaat cagaatatga
    10981 aaaaccacaa ggaagaagta aggagagtct tggaagaaga aaactcaata ggttttcata
    11041 gtgtaagtca tgatatacat aagttatata aaagcccaga agtaacagtt ggggaattta
    11101 atacatgcaa taatactcta caagaaataa ctggtcagag ttcaaaatta ataagattac
    11161 catacggaag taaaccatat atgccagaag gttcatataa taaacttatg gcaaatgatt
    11221 atttaatttg ggactggaat ttggatacac aggattggaa gtcttctaca agtcagatag
    11281 taagtaatat attatattat ggaagaaaaa gagatgaatt agtagtatta attcatgaga
    11341 aagagcaaag tttaaatgct ttaaataata ttataagaat cttaaaagag agaggatata
    11401 caatattgcc aattacagaa aatataacac ctaaaaattt ctggcaagaa aacttataat
    11461 aaatttaaat atagtaaaag ataaagtaaa ttatttataa aataaaacat ataatataaa
    11521 aaatacgaat aagaggtata ctgtaaggat aatataatat aaaattaatg agaaagcatt
    11581 aaaataaaat aatcttgcaa ttattgacat tataagacaa aataactgta aaaacatatt
    11641 ttaaaatata acaactatat aaaagagaat aaacataatt aagaaaattc agaaaaaata
    11701 aaataaaaat aattgacaaa gagaataata tttgtatata ataatatgta atattaaaga
    11761 ctgagaagaa gaggagtaag taaatactac taaaagagag gaaaattcac tggctgtaag
    11821 attttctaag tataagattt actgaatgta gctttggagt ttctaaactg aactataagt
    11881 aagtttagac ggttagaacc gttaaattct ttgagagtca aagttgataa aataacatta
    11941 accaataatt taattatatg ttaatgtatt ttaagctttg gaattaaggt ggtaacgcga
    12001 gcttttcgtc ctttttaaag aggatgaaga gctctttttt atttctaaag atatttaaaa
    12061 aatttattta taaaatacag gaggcaggac aatggaaaat acgaatttat caaaaactta
    12121 taacccaaaa gattttgagg ctaggctata taaaaaatgg atggatgaag ggtattttaa
    12181 atctaaacca aatccagata aaaaaccatt tacaataatg atgccaccac caaatataac
    12241 agggcaattg catatgggac acgctcttga ccatacactt caagatatat taatcagatg
    12301 gaaacgtatg gatggatatg aagctttttg gcttccagga actgaccatg cttctatagc
    12361 aacagaagtt aaagttgttg aaagaataaa aaaacaagaa ggcaagacaa agtatgaaat
    12421 aggtagagaa gaatttttaa agagagcctg ggaatggaaa gatgaatttg gaggaaaaat
    12481 atctaatcag cttaaacaac taggtgattc ttgtgattgg gataaagaaa gatttactat
    12541 ggatgaaggg tgtaatgaag cagttataga attttttgta agcctatatg aaaaaggtca
    12601 catatacaga ggaaatagaa taataaactg gtgcccagat tgcaaaacta ctttatcaga
    12661 tgcagaagtt gaacatgaag aacacgacgg aaacttttat catataaagt acccactaaa
    12721 agatagtgaa gactttttag aaatagctac aactagacct gagacaatga taggagatac
    12781 tggtatagca gtaaatccag aagatgatag atataaacat ttaataggaa aaacagcaat
    12841 acttccatta gtaggaagag aacttccaat agttgcagat agttatgttg atttagagtt
    12901 tggaactggt gcagttaaga tgactccagc acatgaccct aatgattttg aagtaggatt
    12961 aagacataat cttgagcaat taaatactat gaatgaagat ggaactatga atgaagtttg
    13021 tggaaaatat gaaggtatgg atagatttga gtgtagaaaa gctatcgtag cagacttaaa
    13081 agaacaaggt tacctaatta agataaaaga gcataatcat aatgtaggaa cttgttatag
    13141 atgccataca gtagttgagc ctagattatc agagcaatgg tttgttaaga tggaagaact
    13201 tgcaaaacca gctatagata ttcttaaaaa aggagaatta gaatttgttc cagataaatt
    13261 tgataaaact tatttacaat ggctagaaaa tataagagat tggtgtatat caagacaatt
    13321 atggtggggt catcaaatac cagcttatta ttgtcaagag tgtggagaaa tagtagttgc
    13381 tagaaaaatg ccagagaagt gtcctaaatg tggaagcact cattttaaac aagatgaaga
    13441 tgccttagat acttggttca gttctgcatt atggcctttc tctactctag gatggccaaa
    13501 taagactgaa gctttagatt attattatcc aacaagtgta cttgttacag gatacgatat
    13561 aatattcttc tgggtagtta gaatggcatt tgcaggtatg ttctgtatga atgaaaagcc
    13621 atttgaccat gttttggttc atggattagt aagagattct caaggtagaa aaatgagtaa
    13681 atctttagga aatggtatag acccattgga gataatcgag caatatggag ctgatgccct
    13741 aagatttaca ttaacaactg gaaattcccc aggaaatgat atgagattct atatggaaag
    13801 agtagaattt gctagaaact ttgcaaataa gttgtggaat gcatcaagat ttgtatttat
    13861 gaatattgat gaagatataa ttaaaaacat gactagagaa agtgttaaag aggatttaac
    13921 tctagctgat aaatggataa tatcaagagc aaataatatt gtaaaagaag caactaataa
    13981 catggataag tttgatttag gaatagcact tcaaaagata tacgatttta cttggtcaga
    14041 atactgtgac tggtatattg aaatggttaa acctagatta tatggggaag atgcaaatgc
    14101 taaatctgca gcattatata cattaactta tgttcttgaa aaaatattaa aattattaca
    14161 tccatacatg ccatttataa cagaggaaat atatactcat cttcctactg ttgaaggatg
    14221 tataatagta tctgaatggc caaaatataa tgaagaagat aacatggctg aagaagaaga
    14281 tatgatgaac ttattgatgg aaggtataag aagtataaga aatgtgagag cagaaatgaa
    14341 tgtaccacct tctaagaaag ctaaattgat aataatccca agtgaagaaa aaatagaagc
    14401 tattgagctt ggtaaagact attttataac tttagcatct gcttcaaatg tagagatagc
    14461 aaaagataaa tctaatgttc cagaagatgc agttggagta gttattgatg gtgttgaaat
    14521 atttatacct cttaatgaat tagttgattt tgaaaaagaa attgagagat tatctaaaga
    14581 aaagaaaaag ttagaaggcg aaataaagag agttaatgga aagcttgcaa atcaaggttt
    14641 cttagcaaaa gctcctgaaa gtttaattga agaagaaaaa gcaaaaaaag aaaaatttga
    14701 agagatgatt aaatctgttg aggaaagatt aacaaattta gaatctaaaa taaaataaat
    14761 ataagaatta gtaaaaacat acaaaaagta tatattctat actttttgta tgtttttctt
    14821 tatatatata caaatatgat gttataattc tttacagatg tgttatattt ttattttatg
    14881 ttgtgaatat atgaatttta tgtcttaaaa tttataagca ataaaaattt gatatattat
    14941 gatgtttaat atagggtaat aaatacatat tatatttatt ttactatatt tttttacata
    15001 atatatctag aaaagtatag gaatttatcg taatttaagt ttaaatatta aagagaattt
    15061 ctataagtag tttaatcgac aatttaaatt tataaagaaa tttaaatttg aaagctagtg
    15121 agtacagttt ggaaattgaa ataaagtaaa ctaaatgata ttttagattt ggaggtgata
    15181 ttatgttcag ggtagttata tgtgatgacg aaaaaataca aagaagcata ctaaaagagt
    15241 ttacagaaaa acttttgtct gaaagatgtc taaattatca aatactggaa ttttcctgtg
    15301 gagaagattt aatatccaaa tacccagaaa aaattgatat tatattttta gatatccaaa
    15361 tgaaagatat caatggaatt gaggtggcta aaaaaataag aaagtttgat gaaaaagtag
    15421 aaattatatt tacaacggct ttttcagagt atgcccccca aggttatgag gttcgtgctt
    15481 atagatattt agtaaagcct atagagtata gtaactttgc aatgggtgta aatctttgta
    15541 ttgataacct tcaaagaaag aaggagcgtt atatagtttt aaatagtaaa aaaggattta
    15601 atagaatact gatagattct attttatatg ttgaaactgt aaagagagat ttagtcgtac
    15661 atacaatagg tagagattat aaagctaata tgagcatgaa acaagcagaa aaattactgt
    15721 gtgaaaatgg cttttttaga tgtcatacaa gttttattgt taatttaaga agaatagaag
    15781 atcttaggga taatatgatt actattaatg aacaatttat acctataagt aagtatagac
    15841 ttaaagattt aaaagtagca ttaacaagct tgttatgtga tataatgtat taattttaaa
    15901 aaggtaattg aaaatatcgt aaaaaagatt taatgtcatc acagggctat tgattgaaac
    15961 tgtcttaata tataaaatgt tatttatatt aagacagttt tattatgcaa aatagcagtt
    16021 tatagatttt taatatggtt taatatgtat atgtagaggt tccattatag tagcatgatt
    16081 attaatta
//
LOCUS       DAEMIK010000099        15999 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEMIK010000099 DAEMIK010000000
VERSION     DAEMIK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.099"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(225..1829)
                     /locus_tag="KPK87_002586"
     CDS             complement(225..1829)
                     /locus_tag="KPK87_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2538727.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2215..3141
                     /locus_tag="KPK87_002587"
     CDS             2215..3141
                     /locus_tag="KPK87_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG2538728.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3261..4259)
                     /locus_tag="KPK87_002588"
     CDS             complement(3261..4259)
                     /locus_tag="KPK87_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG2538729.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4363..4800
                     /locus_tag="KPK87_002589"
     CDS             4363..4800
                     /locus_tag="KPK87_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2538730.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            4886..5473
                     /locus_tag="KPK87_002590"
     CDS             4886..5473
                     /locus_tag="KPK87_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG2538731.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5773..6393)
                     /locus_tag="KPK87_002591"
     CDS             complement(5773..6393)
                     /locus_tag="KPK87_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG2538732.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(6401..7168)
                     /locus_tag="KPK87_002592"
     CDS             complement(6401..7168)
                     /locus_tag="KPK87_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG2538733.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVVNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7209..8030)
                     /locus_tag="KPK87_002593"
     CDS             complement(7209..8030)
                     /locus_tag="KPK87_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538734.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     VNIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVSEIKNISEKIKLKKDK"
     gene            complement(8389..10299)
                     /gene="phnW"
                     /locus_tag="KPK87_002594"
     CDS             complement(8389..10299)
                     /gene="phnW"
                     /locus_tag="KPK87_002594"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG2538735.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     DYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEYFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10578..11363)
                     /locus_tag="KPK87_002595"
     CDS             complement(10578..11363)
                     /locus_tag="KPK87_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2538736.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQVVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11580..11810)
                     /gene="dltC"
                     /locus_tag="KPK87_002596"
     CDS             complement(11580..11810)
                     /gene="dltC"
                     /locus_tag="KPK87_002596"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG2538737.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(11829..12977)
                     /gene="dltB"
                     /locus_tag="KPK87_002597"
     CDS             complement(11829..12977)
                     /gene="dltB"
                     /locus_tag="KPK87_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG2538738.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(12977..14491)
                     /locus_tag="KPK87_002598"
     CDS             complement(12977..14491)
                     /locus_tag="KPK87_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG2538739.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14612..15802)
                     /gene="dltD"
                     /locus_tag="KPK87_002599"
     CDS             complement(14612..15802)
                     /gene="dltD"
                     /locus_tag="KPK87_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG2538740.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
BASE COUNT         5230 a         2755 c         1693 g         6321 t
ORIGIN      
        1 caattaataa ctacttaatt gtttaatata gtacatagat aactttaata aatcgacagt
       61 tatttaaaaa ggtagaatca ataaaattat acttataaat cttcaataca ataattaaaa
      121 tttaataaaa atataattgt tgatttaact gtaatcttat ttaattctac ctaacttatt
      181 atataaaact attttttata gattataaaa tatctaactt tcatctactt caaagaatac
      241 aatacttccc cttctcttat agtctgcaat actttaatat cttttatttt catagggtcg
      301 attgtaagtg ggttttcatc taatattatc aaattagcta atttcccttc tttaattgaa
      361 ccttttttat cttcttcaaa atattgatat gctgcattta tagtaacacc ctttaaagca
      421 tctaatgggc ttatcttttc attttctcct atttgtatac ctttttttgt aattctattg
      481 acagcacacc atactgtttc cagcatatta ggtgctatta ctggtgtatc ctgatgaaga
      541 gtgtaaatta atcccttttc tatagttgtt ttaagtgggc taatcttaaa cgctctatct
      601 tctccaaggt ttttgatatg aatatcaccc caataatatg tgtgagctac aaagtatgaa
      661 ggtatcatat ttattacttt catatcatca atctggtctt ctcttactgt ttgagcatgt
      721 atcataactg gtcttatatg attttcacta ctttgctctt ttaagtttaa aactttctca
      781 aacgcatcta tcaactggtc tgctgctgca tctccattac aatgagttaa tagttgcatc
      841 ttttctttta gtgaaatatc tataaacttt tctacttctt catctttgta tataggatat
      901 ccacaataac catcatctga attttcatat ggtctactca accatgctgt cttaccttgt
      961 ggagaaccat ctaagaaaat cttataacct cctattttaa atctatttat atattttttt
     1021 ataaactccc tattattatc tgctacagac ttactttttt tcaaatttac atatcctaca
     1081 acatccattt ttaatttatt ttgatttgcc attgctttta aaatgttaaa ttgattttct
     1141 tccattaaac cttcttgagc tgttgtaata ccatagctta gatatatgtt ctgtgctttt
     1201 tcaattgaat taaaaatctc atttgatgat ggttgtttta ttttagatgc tacattaaaa
     1261 aatgcatttt cttctaaata tccatttggt tcttcactgc cttctactct acctatatgt
     1321 ccaccttctg ggtcacgtgt ttcattggta acaccaagct gttttaaccc caatgtattt
     1381 gcaactccca tatgaccaga agcatgtgaa attagtattg gattttctga agatgcacta
     1441 tcgagtacac tcttatctgg atgcttattt tcttctaaaa aattattatc atatgaaaat
     1501 ccaattatcc agtctccttt ttttatatta ttcgactctt tgaaatcctg tatttttttt
     1561 actatgtctt taaaactggt tgcatcttct aaaggaacta gctttagagt agtagcaaaa
     1621 gctatcaagt gactatgtga atctataaaa gatggcatca atgtcttacc ttgtaaatct
     1681 ataatttctg tatctttatt tttcaaagca aatacttctt cttttgttcc tatctttttt
     1741 attattttgt cttttataag aatagcatct ccacatattg aatcttccat tgtgatgata
     1801 tttccattgt aataaatttt tgctttcata aaattctcct tacgttgtct actttaataa
     1861 atttttttca ttattttctg cttaattata ttatcttctt tataaacttt ttctttagag
     1921 tttaatcata taaaagtttt atttttaatt attgtttgcc aagaagtaaa tactattcat
     1981 tgtaaataag acaaattaat aggctttata ctaattgaaa aaatattata agttgttaaa
     2041 tttaaatatc taaactcaat tatatctgta caattgtaat tttttaaatc atgatgtatt
     2101 attttaaata tacagttaca ttattattca caatataagt atcttagttc tcaaataaca
     2161 tctcatgtaa tctattcaat gcaaaaaatt taaatatttt ttaggaggtt ttttatgagc
     2221 aaggaaaata aaaatgtaag aattgctgtt gtccaggcgt caccagtcat aatggattta
     2281 gaaaaaactg ttgaaaaagc attaggatta attaaagaag ctggtagaaa aggggcaaat
     2341 atcgtagttt tccctgaagc attcatacct gcatatccaa gaggtctttc atttggattt
     2401 gttgttggaa gtagaacaat ggaaggtaga gaagattgga aaagatacta tgataattct
     2461 gtaccagttc ctagtgctac aacagattta ttaggaaaag cagcacaaga agcaggagta
     2521 tatctatcaa tgggtataac agaaagagat ggtaatgata taaattgtac cctttattgc
     2581 acaaacttat ttttctctcc agaaggtaag ctaattggta aacacagaaa acttaaacct
     2641 acaggaacag aaagatgtat ttggggagaa ggagatggaa gtactcttac agtagttgat
     2701 actccatatg gaaaaatggg ttcgttgatt tgttgggaaa attatatgcc attagctaga
     2761 acagctcttt atgctaaggg tgtaaaactt tacatagccc ctactgcaga ttcaagagaa
     2821 gagtggcaag caactatgaa gcatattgct ttagaaggaa gatgttttgt aattggatgc
     2881 aatcaatatg ttgaaaaaaa tatgtatcca actgacctta actactataa agagctagat
     2941 gcagaaccag aaattatgtg tccaggagga agttgtatag ttgacccttt tggaaaatat
     3001 gtagcaggtc ctattttcaa taaagaagaa atgctaatag ctgatttaga cctagaaaaa
     3061 atagtactta gtagactaga ttttgattca gaaggtcact attcaagacc agatgtattt
     3121 gaattaattg ttcatgaata atctttttac acaaacatct acataaatct ttttacataa
     3181 aaaaatgaga tactcataag taaatttcaa gtatctcatt tttgcttaaa acctataatc
     3241 ctaattataa atttattcac ctatattttt tctaaatcaa ctactgttct tccaacatgt
     3301 tttcctgcca ttattgaatc tatactatca tttaactctt ccaatgatac tacatttacc
     3361 atatcatcta aatcatctaa tttccattca tttgataata aatcccatat ttttaatctt
     3421 aagttcattg ggcaatccac agaatctata ccatataaag taattccctt taatataaat
     3481 ggatatactg aacttgtaaa atcaactcct cctgcattac cacaagttgt tactgctcca
     3541 caataattag ttgtctttat tgctgttgct agagtatttc ctcctactgt atctattact
     3601 ccagcccatc ttggtctaag cagaggtctt cctgagttat catccacttc tgacctatgt
     3661 ataacatctt taacacctaa gtttagtaat acttcttttt gttcttctaa ttttcctgtt
     3721 gaaactacaa ctgaataacc taatttaatc aatattttag ttgcaattac agcaactcct
     3781 ccagtaccac cagtgactaa tacatctccc atattcttgt ctacagaatc tataaattta
     3841 tacacagata aagctgctgt aaaccctgct gtaccataat ccattgcttc ttttagtgaa
     3901 agatttttag gaagtttaac tacccaacta gaaggaactc ttatgtattc tgaaaaacca
     3961 ccagaagtat tcattcccaa gtcataacct gttactaaaa cttcttctcc aattttaaaa
     4021 tcatcacaac tactagattc tactatacca gatgcatcaa ttcctggtgt atgaggatac
     4081 tttctcgtaa cacccttatt accatttgca gaaagtgcat ccttgtaatt taaagaagaa
     4141 tattttactt ttattaaaac ttcgccttct ggcaatgtat ccacttctct ttcaactata
     4201 tttcttctaa aatttccttc ttcttctgta atatatagtg atttaaattt tttattcata
     4261 aatttcctcc caatacattt agttttatgt taaactaatt atagtttata aatagtttta
     4321 tgtcaaacta atttaaatag attaaagatt ggagaataat ttatggaaga taaatatatt
     4381 gtacatttca taagtaaaac aaaggctagt atgattaaat tcattgaaaa taaactcagt
     4441 aaaaatggtt taggtgagtt aatacctact catggaaata ttttaactgc actttatgaa
     4501 aataatggaa tacttacaat gaaagaaatt gcagctaaaa taggaaaaga taaatccact
     4561 gttacagtgt tggttaataa acttataaac ctaggatatt tagaaagaca aaaatgtaca
     4621 aatgacaaac gtatcacgta tataaagctt acaaaaaagg cactgcttat agaagatact
     4681 tttaatagca tctcaacaca agttaaagag acggcatatc ataatattac agaaaatgaa
     4741 aaacaagaat ttcttagaat tttaaaaaaa attaatgata attttaaaaa tgctgactaa
     4801 attgagcata attatatctc ctattggaat aataaataaa aagaatcaaa tattgtaaaa
     4861 atttattgtg atagagaggt agaatatgaa tttaaaagat agtaaaacta aagaaaattt
     4921 attaagagct tttgcaggtg agagtcaagc tagaaataga tacaatattt ctgcatctgt
     4981 agcaaaaaaa gaaggattat atataataga gtctttattt aattacactg ctgaccaaga
     5041 aaaatcacat gcaaaacaat tcttttctaa attaaaagaa tttgctggtg aaaatataag
     5101 tatatcagga gcataccctg tagatttata tgacaagaca gcacaacacc ttaaagctgc
     5161 acaacataat gagcttcaag agtgggatga tgtatataaa aactttggaa aaacagcacg
     5221 tgaagaagga tttgaatcta tagctaagtt atttgaaaat atagctgaaa tagaaaaagt
     5281 tcatggtgat agatttggta gatatgctac tgatttagaa gaaggtactt tattcagaag
     5341 aaatgaagat atacaatgga tatgtacaca ttgtggccat atacatatag gtaaagaagc
     5401 tccaaaagct tgtcctgtat gccttcatcc acaaggatat ttcttagatt ttaaaaactc
     5461 attatttgaa taaattaagg caataattct atttaaacaa tttactatat ttatatcaag
     5521 tatttttata aatactgtat tatcttaata acaataaaat gaaccattga tattactata
     5581 agtagaaaat gtagtttaaa tacaatttat agtagttcat catggttcat tttattgttt
     5641 actaatccct ataacctact taaatctatc tttaactttt acacactcac ataaaatctt
     5701 atctttaaac taaatattta aataaagtgt attttaagta tattattata atacattata
     5761 taagataata cactatgata actttatact tatatcattt atttcttttg tcaagtcaac
     5821 ctcaataatc ttaactacat ctttttcaaa ctttactttt ttataatcca gacatcctac
     5881 tacgaatgga actccaaact ttctaaacaa ataaatcttt ccactttcat tgtatattga
     5941 tgtaaaattt tcttctatta tattcactat caattttaac tcttcaaaag ttattataga
     6001 tttaactaaa aacttctcta ttatgctcaa tataagcagt tcttttttac ctatgctttg
     6061 ctctttttta tataaatttt gaaatatttc tttggtataa ttacatataa cttctttact
     6121 gagaataaaa tcaatgtcaa attctttatt aatatccttt ttcataaaca tatttgcaat
     6181 atcatcaaga gaagtctctt ctttcataga caatattaac tctattcttt ctagtatttt
     6241 atctcttgga aagaaagtct cttgacctgt aaaagctgat ttttttataa accattcctc
     6301 tggtattatg ttttttcttt tccatctata taattgacca taagatatat tagttttgct
     6361 caataaatct ttcttagata ttaattcttc cataactttt ctagcccacc tttatactgt
     6421 tttttacagt agaattatct gaaactgata aattatcact gtattcaata tttacaacgt
     6481 cacaattatc tactagagat atgcttttgc ctcgtatatc ttttattgat gcatcttcta
     6541 attcaatagt atctccctct atcaaattac atttaaactt tcctgaaaaa atattaatga
     6601 ttttaattaa tgaaaaagca tctttgcttc ttcctatttt tatattagtt gcccctatct
     6661 ctttacaata agaacctcct cctgaataaa catatatatt ttctccattt aaaagaccat
     6721 cacattttat tgctccatat acctttatat cttcacattc tatatttgat ttatttctca
     6781 tttcgccata gatttttata tcttgtgcat acatattttc atttacactt aattgtcctc
     6841 ttattgtaac attattacat ttaagtttgc catccacttt ctgttgagca gttataacta
     6901 agtcatctat ttctgcatct ccctctatat taagttgacc attcactctt acacgctctg
     6961 ctttaagatt gcctcttata atagtttttc cgttaacaac aaaatttttt atattgatgt
     7021 caccttcaaa ttttgcatct ccaaggactt ttacttcgtt acttttaaca tttccttttg
     7081 atttagagct tcccacgatt tttatatttt catactcacc actacttata aagccttctc
     7141 caagtatttt tatagaccct ttttccatat tattacctca ttttaaattt tatttttaaa
     7201 cctacttatt acttatcctt ctttaatttt attttttcac ttatattctt tatttcacta
     7261 actattgaat cttcatcaat ctctatactt tcactgtact ccaaactttc tacataacat
     7321 cccttgccta ttttaatatt ttttcctctt acaactttga cttcactatt ttctaagctt
     7381 atttcatctg cttctataat atttgcaaac aatttacttt ctgtatactt tctaattgat
     7441 aatagaccta aacttattaa atttgaagac actttttcaa agttgatatg ctttttttct
     7501 agcatacgaa attttttgca ataatctcca tgcccctttt ttatatttac actacttgct
     7561 ccaacttcat taaaattaga agttccattt aaactaatat taacttcttc acaattcaaa
     7621 aatccattac actccaagat tccacttaaa ttgatatttt cacaatctat attctcattt
     7681 gcttttaaat atccttctcc atgaataata tccgatttta tacttccttc tacattaata
     7741 attccactag tatcaatttc aacagaatta atatttcctg tacatctaag aagtcccgat
     7801 atctcaactt ttgatgccag tatgtttcct tctactttac ttaaaccaga tgtaaaaaat
     7861 tcattacatt ctgttgtccc aaaaactttt gctgaaccag aagaatcaaa tttactacat
     7921 tttacacttc cttctatttt gcctgttcct gatatattaa cattatcaaa aattccacct
     7981 ttacattttc caacaccatc aaatcttagt gaacttaatt cttcattcat aatgatatcc
     8041 tttctttacc gtattttatc tttaaaccca atataacaaa acattgttac gttgtcaagt
     8101 gtgataataa tacttaataa agcaactatc atttattttc tataaatagg cataaaaata
     8161 atagggaaat gctttattta aactagaaat ctaatttctt agattgtggt ttaaataaaa
     8221 tatatcccta ttactcaatc tttatatatt cattgtataa acttctaaaa tataagtctt
     8281 taaattctac tgtcaatact ttttatacac tgtttgtgct atcatctcac acacctaaag
     8341 gagtgggctt tccgcactat tctgtaacct acaagacact atcttcttct atctattgat
     8401 aaatttttct attacatcag ctagtctctc catatcagtt ggataaactt cacctatgct
     8461 acctattcta aatgtatcta tatcagtcaa tttacctgga tagataacaa atccattatc
     8521 ttttaaataa gtataaaatt ccataaattc aaactttgca tttttaggat ataagaaagt
     8581 tgttattaca ggtgattgag cattatcatt caccaaagta tcaaatccta aagattttaa
     8641 tcttgaagct attgtaaact gattttcttt atatctagca tatctttttt caacagaacc
     8701 ttcttcttcc aattctaata aagcttgata aaaagctctt acaacatgtg taggtgaagt
     8761 aaatctccat ttaccattat ttttctccat agtctcccat tgcgcataca catcaagaga
     8821 taatgattta gctatacctt tacatttaga taatttctct ttattagcta ttataaaacc
     8881 aaatccaggt actccttgta tacatttgtt agaactacta actaaaaaat ctatattaaa
     8941 atattcaaca tcaatctcaa ttcccccaaa actactcata gcatcaacta tataaatttt
     9001 gttatattct tttgcaagtt tccctacctc ttgtatagga tttaatctac ctgtagtagt
     9061 ttcacagtga accattgata tatgtgttat atccttgttt tcttttaata aattttctac
     9121 tacatttaaa tccactgctt ctatatcttt aaatgtgcag tcaacaaatt gtatgttaag
     9181 ataattacat atatctttca ttctctttcc atatgctcca tttgcaatta caagaagttt
     9241 accatctttt gaaatagtac ttcctattat agcttctact gaaaaagtcc cacttccttg
     9301 cattaataca cttgtgtatt tatctgtatt ttgggttgct agagatacta atcttcttct
     9361 aacatcttgt actaggttat tatattcaac atcccaagta caccaatctt ttaacataga
     9421 tgctcttact gatttagtag tagatagtgg tcctggtgta agtaatatgt aatcattttc
     9481 tggtacaaaa tctgatttaa tagtttcatt ctctattttt attagtatat cttctaactc
     9541 agccatagtt tctattacaa aatgtgcacc tgcctcttta aatttctttg aaactatact
     9601 catcttagct tttaattctt ccttatccat gttctctact tcttcttgtg taagccctaa
     9661 ctcacttgaa ccttttatta cagcaacact ccacattcct gcatttacgc cttcttttac
     9721 atcacttatt gtatctccta cttttaccat agaactcata ggagacaccc ctaaagcttc
     9781 agcattttta tagcacatcc aaggatacgg tcttccttga gatacttctg atggtgttac
     9841 aagaaaatcg ggtgagtatc ccttctttgc tgcatttggt tcaactatgt tcatcatctc
     9901 tcttgtataa cccgttgtag aacctatttt taatccattt tttctcaact tttcaatagt
     9961 ttcaacaaca tgaggaattg gagtcgtata atcttctaat gtctcaaata acattggctc
    10021 aaattcagca taaagttcat ttacatcatc ttcagttggc actttaccaa acttatctga
    10081 ccaaagattt tttattctat ccatttcaca catttctctt atatggtcta tttttaattt
    10141 tcccataggt tttcttgctt cttccattgt tacatctata cctcttcttt taaatatctc
    10201 tataaataca ttaagaggtg caaaacatcc ataatctact gttgttccag cccaatcgaa
    10261 tactactgct tttatttttt ctccatatat ctttttcata tttatagcct ccatagattc
    10321 tttttagaat ttttgtgttt ttaattgttt ttacttttaa agtataactt tcatatgtta
    10381 attatacatc atatttaggt taaatatata ttaaatttct gtttttagtt gtatttagtt
    10441 gtttttattt tgtatcatat tcccataaaa tcctaagtta caatctaaaa tttactttct
    10501 aatcttaatc tatgaactga ctatatttta tgaatttagt tgtattgagc atagttgtat
    10561 tatataaaac taagggttta ttttgcttct atccatgtta tatttatctc atttaactta
    10621 tccttccatt cattcggagg ttctacatca caaataacaa aatctaattc ctctaaatct
    10681 gctatcttat acatactagc aattcctatt ttagaactat cacataatac aaaactttcc
    10741 tttgaatttt ttatatattc ttttgacaac aatgccttat tttggtctat actactaatt
    10801 ccaaaactat caagtatacc ttcactagat ataaaagctt tatctacgta taagtttttc
    10861 atagtatcta ttgacattgg acatgctgtc ctttggtgtt ttaaatttac ttttccacca
    10921 ataaatataa cttctccatc aaacaaacct tgattctcat aagaaatcaa ttttgataga
    10981 actggataag agtttgtaat aatttttaaa ttgtttacag atattatata atctacaacc
    11041 tgcaaattgg tagtaccttc atctatgata attgactcat tgtcatttac aaacttagct
    11101 gcatatttag caatactttg ctttttatct aggttagtat tgtttctctt tatatgttct
    11161 ggttcaatca ctccataatt tatttttata gcaccaccat ataccttttt taatttattt
    11221 tcattttcta attcttccaa atatcttctt attgtttctg atgatacttc taatcttttt
    11281 actaattctt tagtgtttac cttccctttt gaatctaata atttaagtat ttcttttttt
    11341 ctatctatcg aatttaaaga cattcatttt cctcctaaat ataatatatt tttctcctat
    11401 aatattacaa tgatacctaa gatttatgaa atcatattta tgtattaaat tttttactta
    11461 attaaagtta ttttcaaaag actttgtatt atcattaaaa aagtccatca aatattagta
    11521 tcagatggac tttgtaaaat tttattttat aaattaatat ttaaagtcta actactaatt
    11581 tattttcttg tctccaagaa ttttactaaa ttatttacag tagacatatc ttttctctct
    11641 aaatcagttg gttgaagctc tatccctaat ctattttcaa tttcaagtaa aacttcaatt
    11701 atagctaatg agtctaaaag ttccgtctca aataaattaa ggtctaaatc ttctcttatc
    11761 tcatcagttc ctaatacatc ttcaaatatt tctataactg tttcttgcat aaattttcct
    11821 ccttaaaatt aagctaaata acctgagaat atcagcaatc caaaacacac aatatggaat
    11881 gtaatgaaaa tctgcactct ctcgaaccat ttatcttttt tatgtttttt ataaaattta
    11941 gattttcgct gatatatatc agttaacaca agtgccaatc cttggtatac accataagca
    12001 acataatacc aagttagacc atgccaaaat cccattgtta tcatggttat catctgagcc
    12061 acatgagcag cagtagtcct tttcttaaat cttttctttc tcattgatga cataacaaat
    12121 ctagaaaaaa tataatctcc aaaccatctt gaaagactta tatgccatct tgtccagaac
    12181 tctttcatat ctttgctgat aaatggcttg tcaaagttta taggtacttg tattccaaat
    12241 atgtaccctg tacctatagc aaataaacta tatcccgcaa aatcaaagaa caggtataaa
    12301 ctatatgcgt acatataact taaaatattt ataaaactca tatctttagg tattcttgat
    12361 acccaatatg tatttatcaa aaatgctata acaaacttat aacctactcc catgacaata
    12421 ttttttattc ctggcagtaa gtattcttct atatactctt ttctagagat ttgtttttcc
    12481 aagtcctgtt caaatcttct tgacctatca ataggtccag aacttaaagt tggaaaaaat
    12541 aacataaaat aaagcatctt tgaaatttta acttctttta tagctccatc ataaatttca
    12601 ataaccattt gtatagttct gaaatttaag tatgatattc caataaatcc aattattcca
    12661 aaagaagtaa ctggagatat tttatttatt attattggga gcattgatgc aaacaagaat
    12721 ccccaatata tgtatttatt tttagttttt cttcttacat attcatatcc tttaactatt
    12781 attacttcaa gaataataaa tattaccaag tactttagtt ggacatcaat tcctactatt
    12841 aagaatatca taaacaaact tgccaacata ccataatact ttatattctt gcccattaga
    12901 cctaaaatta ctgctggtat tgatgttaat aataatatat aaagataaaa ataatctcca
    12961 tattgtgaaa aaatcattaa atttcctcca taagtttctt tctgtctatt ttaccattga
    13021 tatttgttgg aaactcagag attattgaaa tatttcttgg aatcatatat gaaggtatat
    13081 actttccaag ttcctttttt attatcatcc catttttaat attacttaaa tcattctttt
    13141 catttaactc aacaatacct tttaagtaag caattttttc gtccttatat actggtaaaa
    13201 ctactgcatt ttttacatta tgtacttttc tcaaattatt ttctatatct tctatttcaa
    13261 ttctaaatcc atttaattta atctgaaaat cttttcttcc acaataatat atatttccat
    13321 ctaaaagata tcccatatct ccagttttat aagctctcca ctttacacca tctatttcat
    13381 catagaaaaa tacttcatca gtcttttctt tattattgaa gtaaccttta gaaactgatg
    13441 gtccaattat tataatttca cccttttcat tttctttaag ctcatttcca tcttcatcaa
    13501 gtatttttat tttacaattt gacatagggt atccaactgg aagacttttt tcatcatcta
    13561 tagccttttg agtcatatca tttacactta caccaacagt agcctcggtt ggtccatatc
    13621 cattgataat tcttgtgttt ggaaatctac tcataagttc tttagtaaga ttttttgaaa
    13681 gtgcttctcc tatgaatatc attgactcaa ggttaggaag cattttacta ttaaactctt
    13741 tttcagttat acatactcct gcaaaagaag gagttgacac ccatacagct atatctgaga
    13801 tactaaattg tctaaatagt tctttatagt ctgctaatac atcttttgat attgaaaata
    13861 atgtagctcc atggataaga ccagggtata ttgttgtaac tgaaaggtca aaagaatatg
    13921 ctggttggtt cattattact ttttcacttc catctatgtt tagatatggg cttatccaat
    13981 ctgaaaagct atctaaattg tttgaactta tttgaacccc ttttggcttt cctgtacttc
    14041 ctgaagtaaa tagtatatat gcattttcat catcttttac ccagttttct ttatctaatg
    14101 attttccctg atattcattt attatataat ttaatttatc catatcaatt acattgatat
    14161 ctccaaaatt tctttcatca ctaaagttaa ataatacctt tggttttatc tctttagtga
    14221 cttcaaaaac tctatcaatt ggaaaactaa tatcaagagg cacatacgct ctacctgatt
    14281 ttaaagctcc tatcatacat gccattatca tgttttcttt atttccataa attactatag
    14341 gagtatcttc ttctttgtat acatccttta gaaatactga tatagcatct gaatattcat
    14401 ttaaatcttt ataagaaagt ttatctccat tacacattag tgctgttcta tctgtatttg
    14461 aatatttttt tattccttca ataattttca tataagaatg ctcctttaaa atccgttata
    14521 aataaatggc aattctttaa atggagtaaa tgttagaact cctatcgcca atattataac
    14581 catagttaaa caaatactag ttaacaaatc tttattgttc tttaaatatt ttaaataatc
    14641 tctcacagac atctacccat cctttcgtac caagatgcat tacatccctt aaataatact
    14701 tatctgaccc tttatcttta aggtttagaa cttcaaagcc tttgctttct gctatttttt
    14761 gtgctttatc gtagtactga tttctttctt tttcagaaat accagtcaaa ttatagaatt
    14821 tatccatact tgggattaat acaacaactg gcttaatacc taagtcagta catacattta
    14881 gcatcagttt ataattttca aactccttag atgttaataa atttacatct ttgtctcttc
    14941 ctttgtattg ctctatacca tctttaaaat gctctttata atagtactta tcaatattta
    15001 gtggattttt accaactctt tttttagcat cttcaatggc tttctttctc tcatatgacc
    15061 aatttattgg tctttttctt tttgttgctc tctttttatc tagcttaatt aatcgtttat
    15121 aaagaatacc cttttcttta agagctatac agtactttct tccttgaaaa taaggctcta
    15181 aaagcacctt ttcagctttt tcaggaaatg tcttaggctc atatagctta gcatataatg
    15241 cttctgcttt atactcatca cttccccata ataacttact tgattttttt gcatattcaa
    15301 tcttattttg tttagatatt tttggattat ctaaaaatcg ataaaattgt attggtgaaa
    15361 atctagactg ataatggtga cttgtgactc cgtctttttc cataaaccat tgcatagaaa
    15421 ttaacaagac aactttctta ttatcaatat taggattcat acttcctaat attgcagcat
    15481 cttgtaatgt ctgagtatag gctctcccaa ttgcaaacac cttattttta ctcctatttg
    15541 tattaaaata gtactttgga tgttgttttg ttgaattact aagttctgat gaccccaaaa
    15601 tcataatatc cttttctctt aaaaaatgat tatttgctgt aaccccttta tcttttatat
    15661 cacttaaagt atcatccata attggaagta gatttttctc cctcaaaagt tcatcagttt
    15721 tagaatcaag aaatttgtct aacccaaata aaaatactac gcctattata aaaggtgtaa
    15781 taaagtatat caattttctc atattttctc tcctctaaaa atattcaaat ttatgttatt
    15841 attaatcata ttagttttga tatgtatatt caactacaaa attgtaataa aatcgtcaaa
    15901 actttttttg atactgttac aaaattgtaa aaaaaaagaa aaaaacgcca aaaataaatt
    15961 tctttttagc actttttcat acttataatt tgacatttt
//
LOCUS       DAEMIK010000100        15712 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEMIK010000100 DAEMIK010000000
VERSION     DAEMIK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.100"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(17..157)
                     /locus_tag="KPK87_002600"
     CDS             complement(17..157)
                     /locus_tag="KPK87_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2538741.1"
                     /translation="MAELVKDLVLVSLGMGIGVVLMCIAQVSKMADEEMEEIKKERID
                     KE"
     gene            complement(173..1363)
                     /locus_tag="KPK87_002601"
     CDS             complement(173..1363)
                     /locus_tag="KPK87_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG2538742.1"
                     /translation="MNESTFITALKEKRLSYGVSQTRLAIMAGISREHLNRIEAGKVK
                     LTDDMQDKLMEAVEKFNPDAPMFLLFDYVRIRFPTLDIQHVIRDILKLNVDYMLHEDY
                     GHYKYTEHYYLGDVFVYTSRDEEKGTLLELKGKGCRQFESYLLAQGRSWYDFLMDALV
                     EGGVMKRLDLAINDRAGILDIPDLTAKCNREECVSLFRSFKSYASGELVKHNEQDKAG
                     MGHTLYIGSLKSEVYFCCYEKNYEQYAKLGIPIEEAPIKNRFEIRLKDERAYYAVREL
                     LTYYDAEQTAFSIINHYIRFVDREPEKRKTDWKLNDRWAWFIGKDRPPVKLTTDPEPY
                     TLERTLGWISRQVAPTLKMLKKIDAGNSTSYLKEIEDNAKLTEKHLQIIRQQTADTEE
                     LITE"
     gene            complement(1564..2958)
                     /locus_tag="KPK87_002602"
     CDS             complement(1564..2958)
                     /locus_tag="KPK87_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538743.1"
                     /translation="MNILKYKGKRIRASDKNLVYRFCAGTLLLLFVAVLLLLNMGQLM
                     RTDWEHFSLLDNGLTLSTYNFITILIATGVCALVAFLYYRFFHDSFKKLLHRQKLARM
                     VLDNKWYEAQTVQDSGFFTDLQSRSREKIVWFPKIYYQMDTGLLHIRCEISLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVKAENGGLRLMKNLT
                     WEYDALPHALICGGTGGGKTYFLLTIIEALLQTNAQLYILDPKNADLADLGTVMDNVY
                     HTKEDMIECVNAFYEGMVQRSEEMKHHPDYKTGENYAYLGLPPCFLIFDEYVAFLEML
                     GTKESISLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRENFNFRVGLGRISEL
                     GYDMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQAIGSLVLTRQ
                     DGTATCEAKGDGTD"
     gene            complement(3033..3548)
                     /locus_tag="KPK87_002603"
     CDS             complement(3033..3548)
                     /locus_tag="KPK87_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538744.1"
                     /translation="MLNENIRTLRKAKGLSQEELAIKINVVRQTVSKWEKGLSVPDSS
                     MLVSLAEELDTSVSTLLGETVQEECLNEGDLKSISEKLEVINLQFAKKSERKIKTIRY
                     SLILVCVFIAVVFIALVTMNSEYLAWDFNNPELAIVGTLLHGFEFLFVRLAPFVFIGS
                     VIGIVLTYRKR"
     gene            complement(3722..4108)
                     /locus_tag="KPK87_002604"
     CDS             complement(3722..4108)
                     /locus_tag="KPK87_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2538745.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRVENEDGTTTEEIKRRT
                     YDLKCSQQERTIQVSIPAEIPVKDYPYNAEVELINPVADTVANANYRGADVEWYIKAD
                     DIVLKNKGGQPAGNPQNHVPQGQQKK"
     gene            complement(4124..4450)
                     /locus_tag="KPK87_002605"
     CDS             complement(4124..4450)
                     /locus_tag="KPK87_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2538746.1"
                     /translation="MKLKYIVPDMAQTFGNLEFAGENDVEQQRVNGRFVPVTRSYNLY
                     SDIQRADDIIVVLPAKAGEKRFKYEQKVKLVNPKITAEGKNVRGRGYTNYILNADDML
                     PVEESK"
     gene            complement(4644..7688)
                     /locus_tag="KPK87_002606"
     CDS             complement(4644..7688)
                     /locus_tag="KPK87_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2538747.1"
                     /translation="MKKILKRLATGFLAFATIVTALPTTAVHASEKQYWTESAERVGI
                     VEKVMNDGSIGSTFNEGHMTVEGEDAFCVDINTNFKNGYKTRADASTRMSYDQISDVA
                     LSIEYVNQYVQSHSGLSSQHIYLLKQCVVWQRLSVHLGWQCDNVRASYDEIPKAIQDE
                     VYAGAKAFASENKGRYECGGYIYSGEGQDIGQFWAKLAVGNATLKKTSSNASITDGNG
                     LYSIAGATYGIYSDKDCTKQLATLTTDNSGNTDTVEVKAGTVYIKELSAPAGYKVDST
                     VYSLNVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDAPQGDASLEGAEFTWKFYA
                     GHYTADNLPSEPTKTWVTKTIAEKDSDGTIHYVSRLSDEYKVSGDSFYTQDDKNVLPL
                     GTLTVEETKAPNGYLLDGAYMQANGSEEQIKGMYLTQITEDGDIAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETSDTKGQGSATLKDAEFEIISLNDNAVLVEGKLYNKGEVVKTIL
                     TDIEGVASTSADLLPYGKYRIEESKAPEGYLTDGAEPIEFEITEDGKIVDLTGTDTSI
                     YNQVKRGDLEGVKIGAGTHQRLAGVPFRITSKTTGESHIIVTDDNGQFSTSSDWASHK
                     HNTNAGKTSEDGIWFGTSEPDDSKGALIYDTYIIEELRCEGNKGFELIPPFEIVVSRN
                     NVTVDLGTLTDEYEKEITIHTTATGKDGEKSIVAGKEVTIIDTVTLDGLEKGTKYQLK
                     GWQMIKDENAELLIDGQRVENDYTFTADSEEMKIEMEYTFNASSLGGQNLVTFEELYD
                     LSNEDEPVKVAEHKDIDDEGQTVLITERIISIHTTATSEDGKKEIEAGKDVTIIDTVT
                     LDGLEIGTKYQLVGWQMIKDENAELIINGERVENDYTFTADSESMEVQIAFTFDASEL
                     GGKELVTFEELYDLSNPDEPTKVTEHKDIEDDGQTVTITEVPETPEEPTEPEQPTTPD
                     TPSKTTDAPKTGDNTNIALFLGLLVLSGAGVAGTYFFKRRRS"
     gene            8030..8806
                     /locus_tag="KPK87_002607"
     CDS             8030..8806
                     /locus_tag="KPK87_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG2538748.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITKLQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            8868..10202
                     /locus_tag="KPK87_002608"
     CDS             8868..10202
                     /locus_tag="KPK87_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2538749.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            10698..11624
                     /locus_tag="KPK87_002609"
     CDS             10698..11624
                     /locus_tag="KPK87_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG2538750.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(12015..15554)
                     /gene="nifJ"
                     /locus_tag="KPK87_002610"
     CDS             complement(12015..15554)
                     /gene="nifJ"
                     /locus_tag="KPK87_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG2538751.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMIELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         4488 a         3320 c         2822 g         5082 t
ORIGIN      
        1 aagttctgtc cgaaattcat tctttatcaa tcctttcttt tttgatttct tccatttctt
       61 catcagccat tttgctgacc tgtgcgatac acataaggac aacaccgata cccattccca
      121 gcgataccag taccaagtcc ttgacaagtt ctgccataat ctcaattctc ctttactctg
      181 tgataagctc ctctgtgtcc gctgtctgct ggcggattat ctgcaagtgc ttttccgtca
      241 gctttgcgtt gtcctcaatc tctttcagat agcttgtgct gttgccagcg tctatctttt
      301 tcagcatttt cagcgtaggt gcgacctgtc ggcttatcca gcccagcgtc ctttccagcg
      361 tgtaaggttc tgggtctgtc gtcagcttga caggcggacg gtctttcccg ataaaccacg
      421 cccaacggtc attgagcttc cagtctgtct ttcgcttctc tggttctctg tccacgaagc
      481 ggatatagtg attgatgata gaaaatgccg tctgttctgc gtcataatag gtcagcagtt
      541 cacggacggc ataataagct ctttcatctt tcagtcgtat ctcgaagcgg ttctttatgg
      601 gtgcttcctc aatcggtatt cccagctttg cgtactgttc gtagttcttc tcataacagc
      661 agaagtagac ttccgatttc agagagccga tatacagcgt gtgtcccatg cccgccttgt
      721 cctgctcgtt gtgcttcacc agctcgcccg aagcatagga cttgaaagaa cggaacaggg
      781 aaacacattc ctcacggttg catttggcgg tcagatctgg aatatccagt atgcccgccc
      841 tgtcgttaat cgcaaggtca agacgtttca tcacaccgcc ctcaacaaga gcgtccatga
      901 ggaagtcata ccagctacgc ccctgtgcca gcagatagct ttcaaactgg cgacagcctt
      961 ttcctttcag ctccagaagc gtgccttttt cctcgtcccg tgaagtatag acgaacacat
     1021 cacccagata gtaatgctct gtgtatttgt agtgtccgta atcctcatgg agcatatagt
     1081 caacattgag cttcaatata tctctgataa cgtgctgtat atccagcgtg gggaaacgga
     1141 tacgcacata gtcgaacagc aggaacatgg gagcgtcggg attgaatttc tcgaccgctt
     1201 ccatgagctt gtcctgcata tcgtccgtga gcttgacctt tcccgcttcg atacggttca
     1261 agtgttcacg gctgatacca gccatgattg caagccttgt ctgggatacg ccgtaagata
     1321 ggcgtttctc tttcaaggcg gttataaagg tgctttcatt cagcctgcat acccccaatc
     1381 gtgtatttat agaaatctgt gatattttcc gccgattgtc acagtcagcc atgagtgagc
     1441 gaaaaccctt atgcctagcg ggatttccag actttttgac cgtagtttcc agcggaattg
     1501 tgcccctctg ttagataccg aggggcttct ttcgctggcg tggctgacgc cacaccagca
     1561 aggctagtcc gttccgtcgc ctttcgcttc gcacgtcgcc gtcccgtcct gccttgtgag
     1621 gacgagtgag ccgatagcct gtaaaaaatc atgtcccttt ggtacaaggg gagtgtaaaa
     1681 ctctgatatg acgctcgtac ccacgtcaca atagccacgc cctttgatac gcttctggaa
     1741 gaactgcttc tttacgtcgc ttccaaagag catatcgtag ccaagttcgc tgatacgccc
     1801 caagcccaca cggaagttga agttctctct gataccgtct gaaaaatact ttgcgtcggg
     1861 acgctggcag gcgacaatga ggaaatagcc cgcctgtctg ccaagcatga cgattttctt
     1921 tagctggcta agtaggctta tgctttcctt tgtccccagc atttccaaga acgccacata
     1981 ctcgtcaaag ataaggaagc agggcggtaa acccagatag gcatagttct cacccgtctt
     2041 atagtctggg tggtgcttca tttcctcgct tcgctgtacc atgccctcat agaacgcatt
     2101 gacgcactct atcatatctt ctttggtgtg gtacacgtta tccatgaccg tacccaagtc
     2161 cgcaaggtca gcgttcttag ggtctaagat gtaaagctgg gcgttggtct gcaagagggc
     2221 ttcaatgatt gtcagcagga aataggtctt tccaccacca gtcccgccac agataagggc
     2281 gtgagggagt gcgtcatatt cccatgtaag gttcttcatc agacgcaagc ccccgttttc
     2341 agcctttacc tcatcaatcg tgatacggtt cgctatcatg tcatagagca gggtgtattc
     2401 gatatagccg tcatggagcg ttttgtcggt cagctcacag taaagcccgc tttccagctt
     2461 atcttccagt cgcaaaagct ggtcttgata ctttcccaaa gagatttcac agcggatatg
     2521 gagcaagcct gtgtccattt gataatagat tttaggaaac cagacgattt tttcccttga
     2581 tctgctctgc aggtcagtga aaaagccgct gtcctgtacg gtctgggctt cataccactt
     2641 gttatccagt accatgcgtg ccagcttttg acggtgcagt aattttttaa agctgtcatg
     2701 gaagaagcgg taataaagaa aagcgaccaa tgcacaaaca ccagtcgcta tcaaaatcgt
     2761 tatgaagttg tatgtggaaa gcgtcagccc attatctagt aagctgaaat gttcccaatc
     2821 ggtacgcatg agctgtccca tgttcagtag caggagaacc gccacgaaca gtagcaggag
     2881 cgtacctgca cagaagcggt acacaaggtt cttgtcgctg gctctgatac gctttccctt
     2941 gtatttcaaa atgttcataa acaccccttt ctggatatgg aaaaagcggt caatcgtata
     3001 gactaaccgc ttttgtaata ctggatatga aattatcgtt ttctatatgt gagtacaata
     3061 ccgattacag aaccgataaa aacaaaaggt gctaatctca caaacaaaaa ctcgaaacca
     3121 tgtagtagag tgcctacaat cgctaattct gggttattaa aatcccaagc caaatattca
     3181 ctattcatag ttacgagagc aataaatact actgcaataa atacacacac taaaataagt
     3241 gagtaacgaa tagttttaat tttcctttcg ctttttttag caaactgtag attgataact
     3301 tcaagttttt cagaaatact ctttaagtct ccctcgttca aacattcttc ttgtacggtt
     3361 tctcctagca aggtacttac agacgtatca agttcttctg ccaacgaaac aagcatactt
     3421 gagtcgggaa cggataaccc tttttcccat ttagatactg tttgtcttac tacgtttatc
     3481 ttaatagcca attcttcttg tgaaagtcct tttgcctttc gtagtgttct aatattttcg
     3541 tttaacataa cctatgcctc ctttgttttg atacaactat taaatatcaa aacagccgtt
     3601 gccacaagca acgcagatta acatctgtta ttattgcaca aaatcctttt ctatcgctta
     3661 cttcataccc atattatacc aattcaatat aacagctaca atgaaaatct agttagcaat
     3721 tttatttctt ctgctgtccc tgcggaacat ggttctgcgg atttcctgcg ggctgaccgc
     3781 ctttattttt cagcacaatg tcgtctgcct tgatgtacca ttccacgtct gcccctcggt
     3841 agttggcgtt tgccacggta tcagcaacag gattgataag ctccacctcg gcattgtacg
     3901 ggtagtcttt gacagggatt tccgctggga tagatacctg tatcgtgcgt tcctgctgac
     3961 tgcacttcaa atcataggtt ctacgcttga tttcctcggt ggtcgtcccg tcctcattct
     4021 ctacacgcac ctcatgtcta agggcagaga atttcaaaac accgaaagtc ttttccttat
     4081 ctaatacaat tccattcgct aatctcataa tctgataccc cctttacttg ctttcctcga
     4141 ctggaagcat atcgtcagcg tttaaaatat agttggtgta gccacgacct cgcacgttct
     4201 ttccctcggc ggtaatctta ggattgacga gcttgacttt ctgctcatac ttgaaacgct
     4261 tttcaccagc ctttgcggga agcaccacga taatgtcgtc ggctctctga atgtcggaat
     4321 acaggttata gcttctcgtc acgggaacaa agcgaccatt gacacgctgt tgctccacgt
     4381 cattttctcc tgcaaactcc aaatttccaa aagtctgtgc catgtcaggc acgatatatt
     4441 ttaatttcat gtgtttctac ctcatttctt tctgtgattg atatgtgggg agctacactc
     4501 cccacgcccc tagtggactg ttcttttccg tcaatccgca agcagattaa cagaaaagaa
     4561 acagtttaaa ttttcatttc ctgttacatc agaacatacg gcggggaacg ggatttcttc
     4621 atcatcataa gcagaccgcc cgattaagag cgtctacgct tgaagaagta tgttcctgcc
     4681 acgcctgcac cagaaagaac aagaagccct aagaataagg caatgtttgt gttgtcgcct
     4741 gtctttggag cgtccgttgt cttgctcggt gtatctggtg tggtcggctg ttctggctct
     4801 gttggttctt ctggcgtttc tggtacttct gtgattgtca ccgtctgccc gtcgtcctca
     4861 atgtccttat gctctgtaac ctttgtcggt tcgtctggat tgctcaaatc gtataattcc
     4921 tcaaaggtca caagctcttt cccgcccagc tcggaagcgt cgaatgtaaa ggcaatctgg
     4981 acttccatgc tttcactgtc ggcagtaaag gtatagtcat tctcgacacg ttcaccattg
     5041 atgataagct cggcgttttc gtccttaatc atctgccagc ctacaagctg atactttgtg
     5101 ccgatttcca atccgtcaag cgtaaccgta tcaatgattg tcacgtcttt tcctgcttca
     5161 atctctttct ttccgtcctc gctggtcgct gtggtatgga tagagatgat acgctctgtg
     5221 ataagcaccg tctgtccctc gtcgtcaatg tccttgtgtt ctgccacctt tacaggctcg
     5281 tcctcattgg aaaggtcgta aagttcctca aaggttacaa ggttctgacc gcccagagaa
     5341 gaagcgttga acgtatattc catttcaatc ttcatttctt cgctgtcggc tgtgaatgtg
     5401 taatcatttt ctacacgctg tccgtcgata agaagctcgg cgttttcgtc cttaatcatc
     5461 tgccagcctt tcaactgata ctttgtgcct ttttccagtc cgtcaagcgt aaccgtatca
     5521 atgattgtta cctctttgcc agccacgata gacttctcgc cgtccttgcc tgtggctgtg
     5581 gtatggatag tgatttcttt ctcgtattca tcagtgagtg tccctaagtc aacggtcacg
     5641 ttgtttctgg acaccacgat ttcaaacggt gggataagct caaagccttt gttgccctca
     5701 cagcgtaatt cctcaatgat ataggtatcg taaatcaaag cacccttgct gtcgtctggc
     5761 tcgcttgttc caaaccagat accgtcctcg ctggtctttc ctgcattggt gttgtgcttg
     5821 tgggaagccc agtcagaaga agtggagaac tgcccgttat catctgtcac gataatgtgg
     5881 ctctcacctg tggtcttgct tgtgatccta aacggaactc ccgcaagacg ctggtgtgtg
     5941 ccagcaccaa tcttgacacc ctccaggtcg ccacgcttca cttgattgta gattgatgtg
     6001 tctgtccctg taaggtcaac gatttttccg tcctctgtga tttcaaattc aatcggttct
     6061 gcaccgtccg tcaaatagcc ctctggtgct ttgctttcct cgatacggta ttttccataa
     6121 ggtaagaggt cagcggaagt agaagccacg ccctcaatgt cggtaaggat tgtctttacc
     6181 acttcgcctt tattgtagag cttgccctcg acaagtacgg cattgtcatt taaggagatg
     6241 atttcaaatt cagcgtcttt caaggtcgca cttccttgac ctttggtatc gctggtttcc
     6301 aaatctcttt tctggatttt cacaccgcca cggataacct tgtctgatac atggtactga
     6361 ttgcttccag acagtacggc aatatcgccg tcctcggtaa tctgtgtaag gtacattccc
     6421 ttaatctgtt cctcgctacc gtttgcctgc atatatgcac cgtcaagaag atagccgtta
     6481 ggtgctttcg tttcctcaac ggtcagcgtc cctaaaggaa gcacgttctt atcgtcctgt
     6541 gtatagaagc tgtccccaga tactttgtat tcatcagaaa ggcgggaaac gtaatggata
     6601 gtgccgtcgc tgtccttttc agcgattgtc tttgtcaccc atgttttggt aggctcgcta
     6661 gggagattgt cagcggtata gtgtcccgca tagaatttcc atgtgaactc cgcaccctct
     6721 agggaagcgt cgccctgtgg agcgtctttc tgtgtttcca tgtcaatctt gaaaagctcg
     6781 ataagggtat ctgtaacctt tggcgtatcg gatacgttca aggtcgctgt cttgcccgct
     6841 tccacattta aggaatatac agtgctgtct accttatagc cagcaggagc ggaaagctct
     6901 ttgatgtaga ctgtaccagc ctttacttcc acggtatcag tatttccgct gttatcagtc
     6961 gtaagggtgg caagctgttt cgtgcagtcc ttatcagaat agataccata agtcgcacca
     7021 gcgatagagt aaagcccgtt cccgtcggta atgctggcat tactggaagt ctttttaagg
     7081 gtggcatttc ctacggcaag tttcgcccag aactgtccaa tgtcctgtcc ctcgccagag
     7141 tagatataac cgccacattc atagcgtccc ttgttctcac tggcaaaggc tttcgcccca
     7201 gcgtacacct cgtcctgtat cgcttttggg atttcatcat aggaagctct gacgttatcg
     7261 cattgccagc ccagatgaac gctcaatctc tgccatacga cgcactgttt caaaaggtag
     7321 atatgctgac tgctcaatcc gctgtggctc tgcacatact gattgacata ctcgattgag
     7381 agggcaacgt cggaaatctg gtcgtagctc atgcgtgtgc ttgcgtcagc tctggtctta
     7441 tagccgttct tgaagttggt gttaatatct acgcagaaag cgtcctcgcc ctcaacggtc
     7501 atgtgtccct cattgaatgt cgaaccgata gagccgtcat tcattacttt ttcaacaata
     7561 ccgacacgct cggcactttc tgtccagtat tgcttctcgg aagcgtggac tgccgttgtc
     7621 ggcaaagcgg taacgatagt cgcaaaggca aggaagcctg tggctaatcg cttcaatatc
     7681 tttttcatag cttttaaaat tctcctttcg tttggatata gaaaaagccg tccatttctg
     7741 aacggctgaa aatagaaaag gaacgtcaat atcggcgttc cataatctac ttacaatatt
     7801 caatttttag tttatcaata ctgatgtgct gtaccatatc tatcagagct gggacttcgt
     7861 atcgagttca aggcttgctc gtttgtagta aattcgtggt aaattcaaag tttgaagtcc
     7921 tcaaaaaata cgataaatac agtgttttag gggataatcg gactttttag aaagatttta
     7981 ttgcttaaaa tattaatata aaaataaaat aaaattggag gaaatgaaaa tggttaaaga
     8041 taaagttgta ttcgtaacag gagctgcaag tggaataggt aaacaaatag ctgaatcgtt
     8101 cttaaaaaat ggtgctaaag taatgttctc tgatataaat caagaagctt taaataatac
     8161 cataacaaag ttacaaaaaa aaggttatga ttgcatgagt gtaaagtgtg atgtaactaa
     8221 ggaagaagaa attaaccacg ctatagacaa aactgttgaa aagtatggca gattagatat
     8281 attaataaac aatgctggac ttcaacatgt ttctatgata gaagatttcc caactgataa
     8341 atttgaattt atgataaaaa taatgctgac agcacctttt atagctacta aaaaagcatt
     8401 tcctataatg aaaaaacaag gctttggtcg tataataaat atggcctcaa tcaatggtgt
     8461 aataggattt gctggaaagg ctgcttataa ctcagcaaaa catggactta ttggactaac
     8521 aaaagttgct gctcttgaag ctgccgattc tggtataaca gttaatgctt tatgccctgg
     8581 atatgtagat actccacttg tcagaggtca gttcaatgac ctttcaaaaa ctcgtaatat
     8641 accattggaa aatgtcttag aggaagtttt atacccactt gtaccacaaa aacgcctatt
     8701 agatgttcaa gaaattgccg actatgctat gttcttagca agtgataatg ctaagggtat
     8761 tacaggacaa gcttgcttac tagatggagg atatactgca caatagttat tatatttagg
     8821 gcttgaagaa agtattcttc aggtccttct actaaggagg gatatttatg agtattatcg
     8881 cgccaattgg tataattatt ggtatcatag ctattatata tttttcagtt aaagaaattc
     8941 atataactat agctgctcca atagcaactt taatagttgt tattttaaat aaaatggatg
     9001 ttgtaacgtc aatgctaggt gctgacaaaa acaattatat gggtgcactt ggaaactata
     9061 taatgagtta ttttgcaata ttcttattag gctcaatact tgctaaattt atggaagaaa
     9121 gtggagctac tgtatcaata gctgatttta ttttaaataa atttggctct aatcatcctt
     9181 atagagtttt agttgctata tttatagtaa gttttatatt aacgtatggt ggtataagct
     9241 tatttgtagt tatgtttgcg gtcattccac ttgcaagaac tctatttaaa aaacttgata
     9301 tttcttggaa ccttattcag ataccacttt ggcttggtat tgcaacaatt actatgacta
     9361 taattcctgg aacaccagcc attcaaaatg ttatacctat tcaatatcta aatacgtctc
     9421 ttactgcagc tactatacca agtattgtag gtagtatagg ttgtgctaca tttggacttg
     9481 tctatatgaa atatgctcta aacaaaagta ttagaaaagg tgaaacttat gcaacttaca
     9541 cttcagaatt agaagaagaa tctttagata gagaattacc aggatttttt gcaagtattt
     9601 ttcctctatt agcactcgta attatagcta taactggaag tacttttggt tcagaattta
     9661 tgaaaaaaaa tgtaatttac atagctttaa tagttgcaat tcttttatct gcttttttat
     9721 tccataaatt tctatcatct aagatacaga cattgagcat aggtgctagt ggttctatag
     9781 gtcctatatt tgctacatct tctgcagttg catttggagc agtaataatg gctgcacctg
     9841 gttttgatgt atttagcaaa cttataatgt caataccagg tagtccactt ataagtctta
     9901 ctgttttgac ttcaacaatg tcagcaataa cagggtcctc ttctggtgca ttaggaatag
     9961 taatgccaaa ctttgcagat tattatttaa gtactggtct aaatccagaa ttaattcacc
    10021 gtgttgctac aatagcttca aatatactaa ctatagttcc acaaagtggt gtttttctta
    10081 cattcttaag cttatctaag ttaacacata aaactggatt taaagaaact ttcattgtag
    10141 ttgcagttgg ttcactaata gcagaaatta ttgtaattac attaggatta ctaatgtatt
    10201 aatataaaac aagttaaaat tcataatatg aaaggtggtg tctcaaagtg aactttttac
    10261 ttaatgagac actcttttta tatgaaataa catatgccta tcatttaaac aataaaaaag
    10321 gccttatctc tattttgaga cagacccttt ttatttctta aagatttaaa taattataat
    10381 ttgttgctaa tacgaaagca tctactgaat aaatttaacc aaataaaaaa aaatttatca
    10441 taactataaa tttacgttat ggaattatta taaaaatatt attttctcta actaagtaat
    10501 tccatactat acaggctttt aatacaaatt gtcaaacaca ctgaaatata cctatttatt
    10561 ccatatatat cttttttaca tatctttaca gtaaaaaaag tcttaatctt atgttagtat
    10621 agattcacgg gtaaccccct acactaaagg ttacaaatca gtaacatttt tagaattttt
    10681 agattaagag gtgtttaatg atgagaagaa atataccaac aaaattaaaa actatattag
    10741 ctttaggagt gttaacttta gcaacagctt caagtgctgt tactgcaagt gcttcatcac
    10801 ttgaaaataa tgtaaatata agtaacaaaa gtttagtata tagtaatact aaatacaagt
    10861 tttctgcaaa accaaattgt tctgtaaaac cagaacttaa gcctgataaa gataactctt
    10921 gtaaggataa gaatcataat gacaagaatt gcaacaacac taataaacct gaggacaaca
    10981 ataattcaga ctctactaat aagcctgata acaataaacc tgagtctaat aaacctgaag
    11041 acaacaataa ttcaggctct actaataaac ctaataataa taaacctgag tctaataaac
    11101 ctgaagacaa caataattca ggctctactg ataaacctga taacaataaa cctgagtcta
    11161 ataaacctga agacaacaat aattcagact ctactaacaa acctgatgat aataacaact
    11221 caggctctac aagtgaaagt ttttcagctt accaaaaaga agttgttgac ttagtaaata
    11281 tagaaagagc taaagctgga ttaaatcctc ttactcttga ttcttctata tcaaatgttg
    11341 ctacaaaaaa atctcaagat atgattgata acaactattt ttcacacaat tctccaactt
    11401 atggttctcc atttgacatg ttgaaaaaat ttggaataag ctataagaca gcaggtgaaa
    11461 atatagctat gggtcaaaaa actcctaaag aagttgtaaa cgcatggatg aattctgaag
    11521 gtcacagaaa aaatataatg aatccaaatt tcagtaaaat aggtgttgga gtagcacaaa
    11581 aaagtggtgg ttcaatttat tggacacaaa tctttgtagg ataaaatact ctttttccat
    11641 acatatcaag agagtatctc taagtgataa aaaacacaaa tgagatactc ttttgtgtct
    11701 attttaaaat tatcaaatcc aaatatacat actataaata ttatttgttc tcttaatttt
    11761 agtgtgtaaa acaatatata aaaagtattg gcaagatgaa aatggaaaac ttaaaaaaga
    11821 tatttaatag gtaaaaacaa aatcttaata tcttcataaa aaaaggctgt ctcaaaatag
    11881 agataaggcc tttttcattg ttgaaatgat aggcatatgt tatttcatat aaaaagagtg
    11941 cctcatgaac taaaaagctc actttgagac actctctttt atcaattcta tataagcatt
    12001 ataatacttt aatattattc agctaatttt ttgtatcctt catatctttc tttagcatca
    12061 gcttcagctt tagcaaataa tccatcagct tcctctggga attgtttagc tatagcagaa
    12121 tatcttactt gtgcaagtat gaaatctttg aagcttgcag ttggttcttt tgaatctaaa
    12181 gtgaatgggt ttttaccttc agcttttact tctggattga atctgtataa atgccagtat
    12241 ccgcaagcaa cagcttgtgc ttcgttagct acagttcttc ccataccttc ttttaatcca
    12301 tggtttatac atggagcata agctattatt aaagatggcc catcatgttt ttcagcttct
    12361 ataacggctt tgataaattg gtttttatca gcacccatag caacttgagc tacatatacg
    12421 tttccatatg tcatagccat cattcctaaa tctttctttc tagatttttt acctgaagca
    12481 gcaaacttag ccatagcagc agctggagta gctttagaag cttgacctcc tgtatttgaa
    12541 tatacttctg tatcaaatac aagtacattt acatcttcac cagatgcaag aacatggtct
    12601 aatccaccgt aaccgatatc atatgcccaa ccgtctccac ctagtatcca ttgagatctc
    12661 ttaactagat aatcttttct atcttttata gcttttacta attctttagc tttttcatct
    12721 gtacaatcac aagctggttt ttctaataat tcaacaacct tagcacttgc tactttagaa
    12781 gcttctccat catttctaga atctaaccac tctgtaaata ctgctttagc atcttcacag
    12841 atatccatag aaactaactc tatcatagca tctactattt tatttcttat ttgcttaaca
    12901 gctgtgtaca taccaaatcc atattcagca ttatcctcga ataaagagtt tgcccaagat
    12961 ggacctttac cttcatggtt acaagtataa ggaattgatg gtgcagaagc tccccatata
    13021 gaagaacatc cagtagcatt agctatcatc attctatctc catataattg tgtaactact
    13081 ttaatgtacg cagtttctcc acatccagca catgctcctg aaaactccat taatggttgt
    13141 ctaaattggc ttcctttaac agtgtttgca ggcataacat caccttttgg agctacctca
    13201 tcaggattta ctgcaaatgc ccaattatct aactctgtat caagttgagt atctattggc
    13261 ttcataacta aagctttttc tttagctgga catatgtcag cacagtttcc acatcctgta
    13321 cagtcaagtg gactaagttg cattctatat tttaatcctt ctaatccttt acctaatgct
    13381 tttttagctt caaatccttc tggagcatta gctaattctt cttcagtaac taatactggt
    13441 cttatacatg aatgaggaca tataaaagaa cattggttac attgaataca gttatctatt
    13501 atccattcag gaacatcaac agctatacca cgtttttcat aagctgcagt accacaaggg
    13561 aatgttccat cttctatacc attgaatgta cttactggta agttgtttcc ttcttgtgca
    13621 gtcattggtc ttagtatatt ttttataaat tctggttctt taacttctcc tttatctgca
    13681 tcaacagcat tttcccaaga agctggaact tcaactttaa ctaaagcatt tattcctgca
    13741 tcaacagcag catagttcat attaactatt ttttcacctt ttttaccata agctttaact
    13801 atagaatctt ttaagtattt aacagcatct tcttctggta ttatttcagc taatttaaag
    13861 aatgcagatt gcattatcat gtttattctg tttccaagac ctatttcttg tcctagttta
    13921 acagcgttaa ctgtatagaa ttttatatca tttttagcta tatattgttt catatgagct
    13981 ggtaaatgtt cttcaagtcc tgcttggtcc catatagtgt taagtacaaa tgtaccacct
    14041 tttttaagac cttttaataa atcatattga taaacatatg attgtttatg acaggcaata
    14101 taatcagctt catctattaa ataagtagct cttattggag tatcaccaaa tcttaagtga
    14161 gacatagtta taccaccaga ttttttagag tcataatcaa agtaagcttg agcatatttt
    14221 tcagtatggt caccgattat tttgatagct tgtttattag ctccaacagt accgtcagaa
    14281 cctaatcccc agaacttaca tcttatagtt cctttagaag ctatttttat tggctcagat
    14341 ggagttaaag aagtattagt tacatcatca actataccta ctgtaaatcc atttttaggt
    14401 tgttctgaaa ctaagttatc aaaaacagtt tttatatctg aaggagtagt atcttttgaa
    14461 cctaatccgt atcttccacc tattatcata ggagcatttt cttttccata gaatacatca
    14521 cgtacatcta agtataatgg ttcaccagtt gaacctggct ctttagttct atcaagtaca
    14581 catattcttt caacagtaga tggcatagca tctaagaaat gtttcattga gaatggtcta
    14641 tataaatgaa ctttaactaa accatatttt tctcccttag catttaagta atctatagtt
    14701 tcttctattg tttctgttac tgaacccata gctattaata catatttagc atcttctgct
    14761 ccatagtaat caaataaact atgttttcta cctgttaaat tactcatttt ttccatgtat
    14821 ttttcaacta tacctactat attttgatag tatttattag aagcttctct agtttggaaa
    14881 tatatatctg ggttttgagc agtaccacga gcaactggat gatttggaga taaagcatta
    14941 tctctaaatg cttgaactgc ttcaaagttt aataaacttg cataatcttc attttctagt
    15001 aattcaactt tttggatttc atgtgaagtt ctgaatccat caaagaaatg tataaatggt
    15061 aatctacctt ctattgcagc taaatgtgca acaggtgcta tatcagctac ttcttgaact
    15121 gaaccagaag ctaataatac acatccagtt tgtctagcag ccataacgtc ttggtgatcc
    15181 ccaaatattg ataacgcttg agatgctaaa gcacgagcac ttacatggaa tactcctggt
    15241 aataactctc ctgcaacttt atacatgtta ggtatcatta ataataaacc ttgagatgct
    15301 gtaaaagtag aagttaaagc tcctccttgt aaagaaccgt ggaatgttcc tgatgcacct
    15361 gcttctgact gcatttcaac aacattaact gtttgtccaa atatattttt tcttccttgt
    15421 gatgcccact catcaactac ttcagccata gttgaagatg gtgtgattgg atatatagct
    15481 gctacatctg taaacgcata ggcaacatga gctgcagctg tatttccatc aagtgttttc
    15541 ataaacttag ccataatatg tatcctcctt gatagctaca attgtacttg tttataacaa
    15601 gtctattttt ttatatttac atataattta cacaacaaaa ctataaacac acatatagtc
    15661 gcgcaaatac tctatcttct aatataatac tacagattag ataaaattcc tt
//
LOCUS       DAEMIK010000101        15708 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEMIK010000101 DAEMIK010000000
VERSION     DAEMIK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.101"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            10..786
                     /locus_tag="KPK87_002611"
     CDS             10..786
                     /locus_tag="KPK87_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2538752.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(878..1630)
                     /gene="xth"
                     /locus_tag="KPK87_002612"
     CDS             complement(878..1630)
                     /gene="xth"
                     /locus_tag="KPK87_002612"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG2538753.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1791..2741)
                     /gene="galT"
                     /locus_tag="KPK87_002613"
     CDS             complement(1791..2741)
                     /gene="galT"
                     /locus_tag="KPK87_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG2538754.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3126..4322
                     /locus_tag="KPK87_002614"
     CDS             3126..4322
                     /locus_tag="KPK87_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2538755.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4434..5774)
                     /locus_tag="KPK87_002615"
     CDS             complement(4434..5774)
                     /locus_tag="KPK87_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2538756.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(5913..6665)
                     /locus_tag="KPK87_002616"
     CDS             complement(5913..6665)
                     /locus_tag="KPK87_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2538757.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(6813..8390)
                     /locus_tag="KPK87_002617"
     CDS             complement(6813..8390)
                     /locus_tag="KPK87_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2538758.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(8802..9254)
                     /locus_tag="KPK87_002618"
     CDS             complement(8802..9254)
                     /locus_tag="KPK87_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2538759.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(9565..11010)
                     /locus_tag="KPK87_002619"
     CDS             complement(9565..11010)
                     /locus_tag="KPK87_002619"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG2538760.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(11154..12062)
                     /locus_tag="KPK87_002620"
     CDS             complement(11154..12062)
                     /locus_tag="KPK87_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2538761.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            12319..13143
                     /locus_tag="KPK87_002621"
     CDS             12319..13143
                     /locus_tag="KPK87_002621"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG2538762.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            13183..13713
                     /gene="lepB"
                     /locus_tag="KPK87_002622"
     CDS             13183..13713
                     /gene="lepB"
                     /locus_tag="KPK87_002622"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2538763.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDENEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(13795..15576)
                     /locus_tag="KPK87_002623"
     CDS             complement(13795..15576)
                     /locus_tag="KPK87_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG2538764.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRVLFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         5311 a         2449 c         1862 g         6086 t
ORIGIN      
        1 ataaacttta tggaatacat aattttcgat ttggaattta accaaggatt tgataaaaaa
       61 ctcaataaaa ctgtatccaa tgaaaagtgt ccttttgaaa ttatacaaat aggagcaatt
      121 aaattggatt ctaaattcaa tataatagat acgtttaata gttatgtaaa acctacaatt
      181 tataaagaca tacatccctt tattagtcgt atgactaata ttaaaaattc tgattttaat
      241 gattcgccta ctttccctga ggtctacaac aattttataa aatttatatc atcacaagac
      301 cctatattat gtgtttgggg agctggagat ttaaaggaat tatacaggaa tatcaactat
      361 cacaagctac catcaaattc cttacctaaa tcttatatta atatacaaca acatgcatct
      421 aaatatttca ataatccagc aggaaaaagc attggcctac aaaatgctat ttctatatta
      481 gaacttgatg aaaaaatgtc ttatcacaat gctttaaatg atgcatatta tactgctaaa
      541 gtttttatta agatatacaa tccaagtata gtcccagata tatatcttta tacaagtata
      601 aaacctaaaa ctataagata ttctaacaaa aaaagagttg attatgataa gttatttgat
      661 gaatttagga aaattttaaa tcgtgaatta actaaagacg agaaaaaaat cattaactta
      721 gcttataata tggggaaaac aaatcaattt acattagaaa atgttaagca aagaaaaaac
      781 aaatagagtt tatttagata aaaaataggc atgtagatta aaaaactagt taactttaaa
      841 tttatatcta cagcccaatt tttatcataa ttaattttta ttttatctct aattcaactg
      901 gacaatggtc tgaacctaat atttcagtgt gtatatccgc acttactaat ctatcttcta
      961 attttttgga tgcacaaaaa taatctattc tccaccctgc attatttttt cttgcattaa
     1021 atctatatga ccaccaacta taaacacctt ctttatcagg attaaaatat ctatatgtgt
     1081 ctataaaccc actgtctaat aatttagaaa acttctctct ttcttcttta gtaaaacctg
     1141 catttttcat attattttta ggatttttta aatctatttc tttatgtgca acgttcatat
     1201 ctccacacac aattacaggc ttatgattat ctagtttggt taaataatct ataaagtcgt
     1261 cttcccatct agttctatat tctagtctct ttaattcaga ttgagaattt ggcgtatata
     1321 ctgtaacaaa gtaaaaatct tcaaactcta gagttatcac tctaccctct ttgtcatgtt
     1381 cttctatatt tattccatac attactttta aaggctcttt ttttgtaaat atagcagtac
     1441 ctgaataacc ctttctctca gcataattcc aatattgaaa atatcctggt aaatctaact
     1501 ctatttgccc ttcttgcaat ttagtttctt gtaaacaaaa tatatctgca tctacttctt
     1561 tgaaaaagtc taaaaatcct ttcccaacac aagctcttat tccattaaca ttccatgata
     1621 taaatttcat acttatactc ctcatctaaa cattgacaca ctccccatag ctaaagatgg
     1681 gggcttttca acaattttat tgtaaattct taattcactt ttattttaac aatgctatac
     1741 tataatttca tctaaaatta ctatttactt aatacctaaa aatatatgtt ttaatcaatt
     1801 tttaactttt ttgttaaatc ttcagggctc attgaagaaa catacatatt tgttccaagt
     1861 tcaaatcctc caaaattata ttttcttggg attatgtgca tatgaacatt aaaatacgat
     1921 tttgtttcaa tctttgttgg atgagtgtga atacataaat taaatggcat atatccattg
     1981 atattgtata acttcttaaa caacttttca aatattgtag aaagctcctg tatattatta
     2041 tcatttaact caccaaactc tattttatct ttaaatatta ttcttacttc tccactatac
     2101 ttggttgcat atggtataag taccaaaaaa gcttctccat tataaacaac tcttctatct
     2161 aaacttattt cattttctat taaatcatca tacaaagagc ttttattgct gtcataatac
     2221 tcctttgaaa ccaacagttc attcgtaatt tcaggtggaa taaaagacat agacaatatt
     2281 tgagcatgtg gatgcatcaa agatgctcct gcctttctca agaagttttt gaagatacta
     2341 acatactcta ctttatcatc tttcgacaaa tctctaaatc tatttctata cataaaaaaa
     2401 acatcttcaa attctttttg actcatgtta taaaaactac gattatgttt atcgctctct
     2461 atcattactt catgaacacc atatacatct cttgccatat catcaattat aggaaattta
     2521 ttgtagacag attttacaag ccatccttct tcactttcta tttcaaataa tgcatcagtt
     2581 gcatgagctt catttccttt gcaaaatggg cattcttcat catattcatt agtttcttca
     2641 tcttcatttg ggatatctgc tgtatctaaa ggtcttttta acctatctgt agcaaatata
     2701 acaacatcat tggtaattgg atctattctt aattctttca taattaacac ctcttagtta
     2761 atagccttta atataattat atgatattta tcgtatctgt taaataaatt tataatattt
     2821 tttataattt tgcatattta ttattatata ttttgtctat agctgaaaga ttataactaa
     2881 aaaatcacta tcagtatatc agaatattat cttactataa attaatttat gaatacataa
     2941 tttagtggca taaacatggg tattttacta taatttgtta aaagtttatt ttaaagctat
     3001 attaattttt tttgatgatt tttacccata agtatgttat tattatcttg taaagatgtc
     3061 ttgtattcaa acatacaatt tacataatac aatatacgta tttaaattaa atagggggta
     3121 ttaaaatgaa ttattcaaac agagttagtg caatgcaagc ctctccaatc agaaaacttg
     3181 ttccatttgc acaagcagca aaagataaag gaatcaaagt atatcactta aacataggac
     3241 aaccagacat aaaaactcct aaaggatttt ttgatgctgt taaaaacttt gatagcgaag
     3301 tcttagaata tgctacatca gaaggtattc cagaattatt agaagcactt caaaattact
     3361 ataaaactta cgatatgaat tttgaaaaag atgaattatt agtaactaat ggtggtagtg
     3421 aagctttact tttcactatg atggcagttt gtgacccagg agataatcta ttagttccag
     3481 aaccattcta tacaaactac aatggatttg gtcaatctgt aaacgtagaa gttaatgctg
     3541 ttacaacaaa ggcagaaaac ggtttccacc ttccatctaa agaagaaata ttgtctaaag
     3601 tagatgataa aactaaggct ataattcttt ctaacccagg aaatccaact ggtgcaattt
     3661 atacaaaaga agaacttaat atcctagctg aaatagctaa agaaaaagat ttatggataa
     3721 tagcagatga agtttataga gaatttgttt atgatgggct tgagtataca agctgtggta
     3781 acttagaggg tgtacaggat agagttataa taatagacag tgtttctaag agatacagtg
     3841 cttgtggtgc tagaattggt tctatcgctt gcaaaaataa aggtcttatc gctcaaatcc
     3901 ttaaattatg tcaaggaaga ctttgtgttc ctacattaga gcaaatagga gctgttgagt
     3961 tatacaaaac tcctgtttct tacttcaaag aagttaatga agagtacaaa aaaagaagag
     4021 atgtattata caatgaatta atgaaggttg aaggtgttat atgtaaaaaa ccaactggtg
     4081 ctttctatat agttgctaaa ttaccagttg aaaatgcaga agatttcaca atttggatgt
     4141 taaaagaatt taacaaagat aatgaaacag ttatggtttg ccctgctgaa ggattctatg
     4201 ctactccagg tttaggacgt gatgaaataa gattagctta catcttaaat gaaaaagacc
     4261 ttcatagagc tgctacatta ttaaaagaag gtttagagca atatgtagct ttacaaaaat
     4321 aattttttaa acttaattta tataaattta atgatataaa atgtcccctt ttcagatatt
     4381 atttgaaaag gggacatttc ttaattaata tattaattaa gacttagacc tgactatttt
     4441 tcattttctt tatatagtgt tggccagtaa tctttattca ctttaattaa agcatctaaa
     4501 acttgtcttg ccttagttgg agagattata gttctattta atgttaaaga taaaatagct
     4561 ttttcataag attgttcaaa gtatgcatca actgctaact tttcataagc tagttgacct
     4621 tctaacagtc ccttataaaa tgttggtata ttacctacat gtaaaggtct cggtccattc
     4681 tttgtaagaa tcgcagctac ttctaccatt gcatcatcag gtaagttgct aatagcccca
     4741 ttattttctg ttataactat ggctatttgg tgtttgttat gagctattgc ttctgcaact
     4801 tcaactataa attccccatg agcatcatta tgcactaaat tagaacctat agatgttcct
     4861 ttttctataa cagatttaca ttcttcaaat acacgctttt ctcttccatt cataacttct
     4921 cttgctctag tacaatttat atttgattta gctacttttt tatcaggata tagataatac
     4981 tgtacatatg tattaggtaa gtaatctggt acatcttcca acatatcttt aaccattcca
     5041 taagtgtcta accaagattt atctctttgt tctgcatcaa caggttggaa tccagagttt
     5101 gctactaaat cttttatctt aggtaccaaa tcttcaccat ttttatcata caaatgtgta
     5161 aaccatccaa agtgatttaa tccaaagtat acaggctcaa atgttcttga atccatgtct
     5221 aacaatcttc cataagacct taacaagtta actggttggt cacacatatt aagtaatctt
     5281 ttatcatctg gaaatactct ttttaatgct tctgcaacaa tagcagcagg atttgtataa
     5341 tttaatatcc atgctttagg agagtatttt cttacagtct taaccatttc tatcatatca
     5401 cctatagacc ttaacccata agcaaatcca ccagctccac aagtttcttg accaaccagt
     5461 ccaaattgta gaggaatctt ttcatctaat tctcgcatct taagaccacc agttctcatt
     5521 tgaacaaata caaaatctac atctataaat gcttcatctg ggtttgtagt atattcaagt
     5581 gttgctttag gatattcttc tttaaataat atttttccaa attcccctat taatctttga
     5641 cgttcttcta ctacatctaa catagttact ttatttagag gaaatctatc actcatttta
     5701 cataaagact ttaaaagtcc tggagtccaa gtactgcctc ctccaactat agctatattg
     5761 tatctttttt tcattgtcat attaatcacc cctgtttttt attaactaac ttgtaagatg
     5821 attataatat ataaagtttt ctacatgaat acttttaggt aatcattaaa aatagttcaa
     5881 ttttagtatt tttttcaaaa ttgtaatttt tttcatctat tgtatttttc aatgtattta
     5941 tgaaacatat attccataag agaaagtgct ggcactctag atgtatagtt aacactatta
     6001 aaattatatt gtggagaaga tacaaataat tttatatcag ctatatttga taatgaattg
     6061 acacttatat ttgtcagaga aattacttta acttgatttg tttttgctat attacatgcc
     6121 ttaataactt gagcagtctc tccagacata gatacagcaa ttacaaggtc atctttagta
     6181 atatttgatg cagccaaata catgagagtt gaatcatcat agtattttac atttacacct
     6241 atagctgaaa aacgttgaga catttcagta catactaatc tagaagaccc aacaccaaaa
     6301 aaatatatat gcttagaatt atgtatttca tttaaacaca tatctatatt ctctacattt
     6361 attaaatttt gtgtatccat aacatcagta aaaatatcac tttgactttt agtaaactcc
     6421 ttatttcttt catttctatt tgaatatgaa aaaactaaaa agtttttaaa ttcactatat
     6481 ccagaaaagc cgagcttctg acaaaacctt actatgtatg cagtcgaatt aaaagtttca
     6541 tttgctatat ctttaatctt tttttgattt attttttcag aattttttag aaaatagttt
     6601 aaaagagata tttcatttgt agataatttt ttttgattta tatctaattt gtctattagg
     6661 ttcatttata cctccataat aatgatttgt agttaaacaa ctttctcata tttattatat
     6721 aaatatacac tatacagcat ttatcttatg tttattatac tataaaaaag acaatacaaa
     6781 ttataaatta gtattgcctt ttaatattta ctttaaatag acattgcaaa agaaacttcg
     6841 tctcttactt gagatacaga tggcccataa attatttgga tatctttatc agatactttt
     6901 ataactccca ttgcacctgt atatttttta aaagcatttt catctacttt actcatatct
     6961 ttaactacta ctcttaagcg agttatacag tttgttatat cttctatatt gtccttacca
     7021 cctaaatatt taattataga taatgctctc ttagtagttt ctccactttg agctatttct
     7081 actttatttg ataaaggttt taaatcaaaa tttatatctg ttatttcttc tcgtcctgga
     7141 gtttgtaagt taaatctaag tatcaaaatc ttaaatacat tataataaac tattgcatac
     7201 actaaaccaa gtattataga ataaatccac tttgtatttg gttgcataac tccaaatact
     7261 actaaatcta ttatgccacc ttgagtattt cctattgcta ctccaaatat atgcatcatc
     7321 ataaatgata tacctgataa aaatgcatgt ataacaaata atattggtga tacaaataag
     7381 aatgaaaatt ccaatggttc cgtaattcct gttacaaatg ttgctaaagc accagctagt
     7441 aatatacctt taaccttcga cctattttca ggctttgcag ttttatacat ggcatatgca
     7501 gcagcaggaa gaccaaatac cattataggt attttccctt gagctaaaaa tctagttgct
     7561 tctgttgaaa aactgattgt ttcagggtca cttagttgag ctaagaatat gttcaatgct
     7621 ccaaatactt cttgccctcc cacaactaaa gaacctccta cttcagtaaa tctaaacata
     7681 gcatttaata tatgatgtaa tccaaatggt attaaaagtc tttcaagaaa tccaaataca
     7741 aatgttccta aatatcctaa actagctata ccaataccta cactctgaat aattgtattt
     7801 acatatggcc atatatatgg tattattatc cccaaaatag agaatactac tgttgttatt
     7861 attggaacaa acctagttcc accaaagaat cctaaataat ctggcagttt tatattatag
     7921 tatttattat gtaatgatgc tgttattata ccaactacaa ttccaccaaa tactcctaat
     7981 tctaatgttt gtatgcctaa agttacagct tgacccattt ctctcatcat gtcaggttca
     8041 gcaagaatcc ccctacaccc taacatataa tttacactag tgtgtaatac taaataacca
     8101 acaaaaccag aaaatgcaga aactgctttt tcttctttag caagaccaac agatattgat
     8161 atagcaaaga atattgctaa gtttccaaaa agtactcctg ttatagcttt tgtaaaaccc
     8221 aatattcctt gaataaaatt atttgctaaa aaaggcatat cactaactat attaggattt
     8281 gaaaatgctg aacttattcc taaaaatata cctgctgcag atattaatgc tattggcaat
     8341 aaaaatgatt ttcctaactg taaaaagaac cctttcattt tttgtttcat ttctatccct
     8401 ccatttttaa atttttaaaa cgttttctat gtcataatta tatattttta ttttgttttt
     8461 gaaactttat aatttgtttt aaaatatttt tacataaata gtaatttttt caaaactgta
     8521 aattttttca aacaaagaca tagtaacgtt gtaacatata taaatttaaa aagagcttag
     8581 aaaataaaat tattgaatat aaaatggaaa aatataaatt acacaaagag atatgagtac
     8641 gtactaacaa atacgaaaat gtataagaaa ttataaaaaa gtacatcaag aaaatttaag
     8701 atgtactttt tagattgtat ttagacttat aattatatag ttaaatgaat aacaaagaat
     8761 taatcttagt actattaaat acaaaaattt ttatattttt tctatctaaa ttcttctttg
     8821 tttaatatat attgaagagt atttattgct tctaaatatt tttttctgtc ttcttcagtt
     8881 aatttagaaa gtttttctcc taaaccatca cttaatttat ccataaaatc agttgctatc
     8941 ttatctccct cttcagtaat gtcaattaga tattttcttc tatcaactaa atctcttttc
     9001 cttgttacat acttcttatc agttaaatca tcaaccatac tagttaaact gcctttttct
     9061 atatttaact tagagcataa atcagtcata cttattactc cataattttt tatgaaaact
     9121 aatgctctta attgtgtttt atttacatta taatctggtg caaatttttt taggtcttct
     9181 aagtacaggc tagaataaat cttaggaaat gtttttacta aaaaacttat ggtatctttt
     9241 actatatagt tcatataatc acctcggttg ttttaaaatt tattaaatta tctaaataac
     9301 tttatttcag tttacattaa aaatattgca agtacaatag tttttgctga atgtttaaat
     9361 ttaattagta atcttatcat ttttttattt ttatttatat tattcctatt aaatattatt
     9421 tatatagcta ttttattaaa ttcatggtat agtaagtaaa gaagaactct tcacagcggt
     9481 ggagagaaga cgctagccca ttaaaatggc tatcttattt ataaataaga tagccgtttt
     9541 taattatatt ttatctgtac aattctatac tcttaggtct acatctactt ttcctaagtc
     9601 ttccttataa tcttttatag caggttctac aacttcattt acaaattcta caacttgttc
     9661 tggagctcta cctataaagt tttttgggtc cattatagat agtatttctt ccttgctcat
     9721 tttaaatgaa tcatcattca ttataagttc aattaagtta ttatccttgc cttcatgctt
     9781 tactctatag gcagctttca tagaatattc tcttattatt tcatgcaatt cctgtctatc
     9841 tccacctctt tttacagctt ccataagtat tgtttctgta gccataaaag gaagttcttc
     9901 atttacacgt ctatttatca tattttcata tactactaaa ccatcagtta catttatccc
     9961 tatttcaagt atagcatctg ctgccataaa tgcttgaggt attgccaatc tcttattggc
    10021 tgagtcatct aatgttctct ctaaccattg agtggcttgt accattgcag ggcttataga
    10081 ttcagagatt atatacttag ataaagatgc tattctctca cttctcattg gattcctctt
    10141 ataagccatt gctgatgaac ctatttgatt tttttcaaat ggctcttcta attcttttaa
    10201 actttgtaaa agtcttatat cattagtcat cttatgcata ctttgagcta tccctgatag
    10261 tatgcttatt acttgataat ctaactttct agtatatgtc tgtccactta ctgcatagga
    10321 agaatggtat cccatctttt tacaaatttt cttatctaac tcttttactt tttcgtggtc
    10381 tccttcaaat aagcttaaaa aacttgcttg agtaccagta gtacctttaa ctcctcttaa
    10441 cttcatgttg tctattctat agtttaaatc ttccaaatca agaattaaat cttgagccca
    10501 taaacaagct ctttttccta ctgttgtaag ttgagcagct tggaagtgtg taaatccaag
    10561 agctggcata tctttgtact taagtgcaaa ttcttttaaa ttgtttatta aatttacaag
    10621 ctttactctt agaagtttta aagcttcatt catttgtata acatctgtat tatctccaac
    10681 atatgcagat gttgctccta aatgtattat tcctttggct ttttcacatt gtaaaccata
    10741 agcttttacg tgagacatta catcatgtct tacttctttt tctatttttc tagcttcatc
    10801 aaaatttata ttatctatat tagctcttag ctcatctaat tgttcttcag ttatattgat
    10861 tcctaactca tgttcacctt ctgcaagtgc aacccataac cttctccatg tagagaattt
    10921 aaattgtgga gaaaatatat aactcatgtc cttgctacaa tatctgtctg taagtgggtt
    10981 tgagtaagta gtatatttat tatctatcat tatattagca ccttccttaa atcttttgtc
    11041 gtgtgattac tgtgtacaca ttagtacata aataattata tataatttta cgaactataa
    11101 caagactttt ttattttttg ttcacattta tatatagtat tttttattta ttactatttt
    11161 ttatttatat ccaaattatc tttttcaatt aagtaatcca ctatttcact atcaacaaaa
    11221 tttaatacat acttatatct aattgcatag tatttatcac tccatccaag ctgatttttt
    11281 acatcacttt catctgcccc aagccttaaa cttattgctg caaatgaatg tctaaaatct
    11341 cttgctgaat attgagaaag tccagctaaa tctaatgctt ttttgactat aagtctgaca
    11401 tttctatctg ttatagaatt actcttttga gttgtaaaaa taaaatcatc actaggtatt
    11461 attacctctg gaagcccaga ataattcctg taatcttcta aaagtttaaa aaagtatgga
    11521 tgtaatttaa ctactctttc ttttcttccc ttacctaacc ttacatatga gttatcgttt
    11581 tcatctacca taaggtcttt ccactttaag ctaactaatt catttaataa acacccagta
    11641 gtaactagac agactattat tactctatct cttatattta attttggtag gatacctata
    11701 agtttattta tttcttgtat acttagaaca tcatcttttg tttttattct tgttactgtg
    11761 ggtttttcta catatctaaa tggatttata tctatatatc cttctttttt taagaagtta
    11821 taaaaactat gtaaataact atatatcttt tcacaagttg atttcgcata tttagtctgt
    11881 atatagtcta caaaattttt acaatcttct ttcgtagcat aaatcaattc tttccctttt
    11941 aaaaattctt taaacaatat aacctttgat aaataatcgt gtttcgtgtt tggatttaat
    12001 tttgtagaaa aaacattaaa agtttcatac tcataatcat ctagtatttt tgtactatac
    12061 ataataactc ctttcctttt cataaaataa aatagctata cttttactat accatatatt
    12121 tccccctcaa atatacccct tattcaatag ataactttta agtttatttt gttatattat
    12181 gaaggtttta tgaaaatatc aatcttccat tgtgcactac acagtactat ggtattataa
    12241 gttagtaaaa ggtggcattt ttttattaat ctatgaataa aatatgctac tataaatttg
    12301 tattggaggt attttgattt ggaaacattt agtttgcttg aaatttttaa ggcagtcatt
    12361 ttgggcatag ttcagggaat aacagaatgg ctaccagtaa gtagtacagg gcatatgata
    12421 ttagttgatg agtttataaa attgaatttt tcaaatacat ttataagtac atttttagtt
    12481 gtaattcaat ttggttcaat attagctgta cttgttatat tttttagaaa attaaatcct
    12541 tttgatagcg caaagaatat taagcagaaa aaagaaacag tccgtttatg gcttaaggtt
    12601 ataattgcag ttataccatc tggagttatt ggaatactgt ttgaagatga tatagataga
    12661 ttattcttta attcaacagt agttgctatt gctcttattg tatatggtat aataatgata
    12721 gggcttgaaa agagaaataa aaaacctaag tataaagatt tttctcaagt tacatataaa
    12781 ttagctcttt gtataggatt attccagtgt ctagctctta taccaggtac atctaggtct
    12841 ggttctacta taataggtgc agtattactt ggtacttcta gatatgtagc agcagagttt
    12901 tcattcttct tagccatccc tacaatgctt ggagccagtg cattaaaact tctaaaagct
    12961 ggatttggat ttactggatt tgaatggctt attttaggtg ttggttctgt agtagcattt
    13021 gttgtttcaa ttgtagttat taaatttttt atggactata tcaaaaaaca tgacttcaaa
    13081 gtatttggtt attatagaat agtcttgggt atagtggttc ttgcttattt ctttctatta
    13141 taatgtattc taatttaata acaaatgata aggagaaaag ttatgagtgt taaaaaagaa
    13201 atatttgatt ggattaagtc aatagctatg gctattgtac ttgcatttgt aattctacaa
    13261 tttataagac cttctattgt aagtggagaa tcaatgtatc ctactttaga tgataaagat
    13321 tatctgattt taaataggat atcatacaag gttggtaaac ctgaaaaagg cgatattgta
    13381 gtttttaaaa ccaatttagt tgatggagaa acaggaaaga aaaaagactt aataaaaaga
    13441 gttatagcta ctgaaggtga cagaataaaa atatcaaatt ctaaagtgta tgtaaatgga
    13501 aaattattaa atgaaccata tatacacaat aactatactt ctggagatat agatactgtt
    13561 gttccaaaag gtaaactatt tgcaatggga gataatagag aaaatagtaa tgatagtaga
    13621 ttccctgatg taggtatggt tgatgaaaat gaagttcttg gtaaggttat ggtgagacta
    13681 ttacctcttg ataatattgg gaaagtagac taaaaaataa gagactgaat tggtaaagtt
    13741 tattttacaa atctcagtct ctttttattt tcttaaatta caaatatttt tttactaaat
    13801 atcttttgat tctgtaataa gtcttattat atcacttgct tcttcattaa aagttgttac
    13861 tatacttagg tagtctgttg ctctactcat agctaaataa accttattta aatctcctac
    13921 aaagtcattt acttgataat cttgttcaat atcatttata gtttgattat ataacatctc
    13981 taattcacat actattaccg ctttatactc taaattcttt attgcatata tatttgctat
    14041 tgtagttcct accttcttac ttatatttgt caaattatct tctgcacata tataaggaat
    14101 ttttgcctct tccaatgctt tttttaacat atattgaaag taaatggttt ttccactttt
    14161 taatttcttc ttattgtaag gataaacgat agcaacttca gaataatcaa gacctttttt
    14221 acttattaaa tactcaattt cccagattac agaacttatt tggtcatcaa ggtcacttac
    14281 ttttattata tcaacagact tatttccacc tctaagtgct ttagtataaa agaaagtact
    14341 aaattctgta tttggtctta attcgttgat atatgagtta gaattatttg aaaatttatt
    14401 tgtaaaatct acaatctctt tagcttgtct ataattttta cttaagataa tcttatcatc
    14461 aaattctata ccattatata attttttaaa tatattcaat ctattagaga tattcaagga
    14521 gttacaagaa catacattaa atatgaattt tgttttatat aaaaactccc ttataaaatc
    14581 tatttcatac tctaaaaagc tttctgcttc atcaataaaa atagccttga acatattttt
    14641 attttttata atgttctgtg cttgttttac aagattatta aaggtctttt cgtaatcatt
    14701 ttttaacata ttataatcta caattaaatt gaatttttta gctagtttaa atataaaaga
    14761 actaaatgtg tgaacttcta aattgttatt atctttgtat aataactcta tcctttctcg
    14821 aagctcattg cacaattgtt ttgtatgtgt aaatatgact aatttatgat gtggatatac
    14881 ttttgctaat tttatagccc tagaaagcat aattgctgtt ttgcctatac cagaaccacc
    14941 ttcaataagt gtattcccat atttaataga aacaacatct ttaatttgaa cgtcttccat
    15001 catagttgct gtatacttat agtcatcatt gtagaatgaa atttttttaa atttttcatt
    15061 tagatgaagt ttatcattta ttatatagta ttctgaacat atgtctagta aaaataaatt
    15121 taagtttatt tcatcattct catcattaag atattcctcc agcgaaccct tattcattat
    15181 atcttgaagc ttatttttat ctattatatt attattaaca aattcttcaa attcatatgt
    15241 ttcctcaact tcaacgtatg gcataatgta aacgtagtta taacttatat ttctattctt
    15301 ttgtcccatt tttagtttca aaagttcata ttcctcattc ataacttcaa gtaactcttc
    15361 ttctaaaata aaaaatatat cttctgttgt atccataaat tttataaata aaactcttcc
    15421 atctttaaca tacaacaaat cggtatttat tcctttaaaa ggagtaactt ttggcactac
    15481 tttaacgtcc tcattgatat tttcgtaaaa tattttctcg aactccttca attttagggc
    15541 gtctaactga gaatttatat ttatgtattg gctcacttac attacctcct tctgttatac
    15601 ttaattatac cattttgaac tatattttta tatatacttt ttataggcaa atttagtaaa
    15661 taaattcaaa ataaatacaa ataaatacaa aactgctaaa ataatttt
//
LOCUS       DAEMIK010000102        15528 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEMIK010000102 DAEMIK010000000
VERSION     DAEMIK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.102"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1601
                     /locus_tag="KPK87_002624"
     CDS             <1..1601
                     /locus_tag="KPK87_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG2538765.1"
                     /translation="YLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDFSVSLNQVNF
                     DSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFEKCKKDIGIN
                     KDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQHFKNKDILIN
                     YIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQTLINFVDNI
                     SRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIALIVFGNIPKL
                     SIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKEKSKTRISPN
                     STYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENSVNWYFQTLD
                     KKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESFYTNKFKFED
                     KNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTYFFATNIQSK
                     DYSNGSSASKITLSILNDKKIYNP"
     gene            complement(1956..3830)
                     /locus_tag="KPK87_002625"
     CDS             complement(1956..3830)
                     /locus_tag="KPK87_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2538766.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(3835..5499)
                     /locus_tag="KPK87_002626"
     CDS             complement(3835..5499)
                     /locus_tag="KPK87_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG2538767.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDVNLLNKTVRLKPFHAVLLKAR"
     gene            complement(5513..6334)
                     /locus_tag="KPK87_002627"
     CDS             complement(5513..6334)
                     /locus_tag="KPK87_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2538768.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVAQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(6433..7197)
                     /locus_tag="KPK87_002628"
     CDS             complement(6433..7197)
                     /locus_tag="KPK87_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2538769.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEIKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(7194..7943)
                     /locus_tag="KPK87_002629"
     CDS             complement(7194..7943)
                     /locus_tag="KPK87_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2538770.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRICRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(8447..9052)
                     /locus_tag="KPK87_002630"
     CDS             complement(8447..9052)
                     /locus_tag="KPK87_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2538771.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            9231..9869
                     /locus_tag="KPK87_002631"
     CDS             9231..9869
                     /locus_tag="KPK87_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2538772.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            10001..10663
                     /locus_tag="KPK87_002632"
     CDS             10001..10663
                     /locus_tag="KPK87_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2538773.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTPGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            10663..11844
                     /locus_tag="KPK87_002633"
     CDS             10663..11844
                     /locus_tag="KPK87_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2538774.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESILLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(12025..14685)
                     /locus_tag="KPK87_002634"
     CDS             complement(12025..14685)
                     /locus_tag="KPK87_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538775.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVISAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSGNYHADAPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(14682..15365)
                     /locus_tag="KPK87_002635"
     CDS             complement(14682..15365)
                     /locus_tag="KPK87_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538776.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         5477 a         2513 c         1849 g         5689 t
ORIGIN      
        1 actatttata tacttttaat caacttggaa gcagtactaa taggacttct atgataaaaa
       61 atagtccaaa ttctataatt aataactcaa atgcattaaa cgatttttca gtatccctaa
      121 accaagttaa ttttgatagt ttaaatattt tattactaat atcatggata attggaattt
      181 taatcatgat agctattact attcattgca acattaaaat aaaaaatatg aaaaaatcaa
      241 tacaaatttt aagagatgag gagatagtat ttatatttga aaagtgtaaa aaagatattg
      301 gtataaataa ggattttata ctatgcaaat cacctattat aaatacacct attacatttg
      361 gatactttaa accacacatt atattaccag ataagtctat atctaaatta tctttaaaag
      421 atataaaata tatcttactt catgaattac aacactttaa aaataaagat atactgataa
      481 attatataat gtgcttcctt caaatattat attggtttaa tcctcttgta tggtatgcat
      541 ttaaagaaat gcgtatagac cgtgaaattg catgtgatat ttcagtttta aaaaagcttg
      601 ataaagatag tcatatagaa tatggacaaa ctctaataaa ctttgtggat aatatttcaa
      661 gaccatcaaa tcttacactt acatcagata ttggtgggtc aaaaaaacaa ataaaaaagc
      721 gactattgaa gataatggat tttcataatg aatcaaatac gttaaaattc aaaagcattt
      781 taatttttac attgatagct ttaattgtat ttggaaatat tcccaaacta tctattatgg
      841 cagatatcaa cgaaaaatat agacctacaa atgaacaaac tgtgtacgag gacttaagtc
      901 agtattttaa aggttttgat ggaagttttg ttatgtatga tttaaaatcc aagcaatatc
      961 atatttacaa taaagaaaaa agtaaaacaa gaatttctcc aaattctact tataagattt
     1021 atagtgcctt attgggattg gaaaatggga taataacaag aaataattca catatccctt
     1081 gggatggaaa gcatcattct aatagttctt ggaatgaaga ccaagactta ttttctgcta
     1141 tggaaaactc cgtaaactgg tactttcaga ctctagataa aaaaacaggt ctaaaaaaca
     1201 tacaaactta tcttaggcaa attggatatg gtaattatga tgtttcaggt ggtatctcaa
     1261 acttttggct agaatcttca ttaaaaatat cacctattga acaggtagaa ttacttgaaa
     1321 gtttttatac aaataagttt aaatttgaag ataaaaatat agaaacagta aaagattcat
     1381 tgttaatatc aaaaaaggat ggggtatcac tttttggtaa aacaggcact gccaatataa
     1441 atggaaaaga tgtaaatggt tggtttatag gttatgtgga aaaaaatgaa aatacttatt
     1501 tctttgctac aaatattcaa agcaaagatt attctaatgg tagttcagca tctaagataa
     1561 cattatccat attaaatgac aaaaaaatct ataatcctta atatatttac tgtacattct
     1621 aaaaaattca cataattttc atattaatta tcaattaaat tcatatacaa ggcttattat
     1681 ataaacatag aagatagtaa aactagctta aaaacataat ataataccct atacaaatag
     1741 tggcaactct ccctcacatc ctcaattgtt gccactattt aattttttgt atgaagttaa
     1801 atatattttc atcatttcaa caatttgggt gcaatacaat ctctattttg aaacaacttc
     1861 tttaattgtt ttagagttta tgaattattt ctcatacttt aaaaagattg aattaatctt
     1921 gataatcttc ttttgtagtc attacatatc acctactact caacacatat tctttgacct
     1981 atttttacat ccttgtttat atccaaaata tttaccttct taccatctgg aaacaatacc
     2041 attgtcaaag tagactttcc taattcgtgt attctcttta aatcaacatt aacaagaaca
     2101 tccccctgtt taaccatatc tccttgttta acagcaacat caaagccatc tccatttaat
     2161 gcaactgtat caattccaac atgaattaat acttctccat tagttccttt aatcccaaat
     2221 gcatgtccac ttgaaaacac tgtttctaat ttacctgaaa taggagaaac aatttttcca
     2281 tctataatat caatagcaaa accatcacct aacatttttt tagaaaatgt ttcatctggt
     2341 acattttcta atgcaacaat tttcccttta gctggagaca caaagttttc attattttca
     2401 ttaaacacta attcttcgtc ttcatcttca tttatagctt ctttaggcac tccaaataaa
     2461 catgttacaa taaagcagaa tgcaaccaca aatattgata ctaataagta tgtgattaaa
     2521 ttatatccat catagatata cattaaataa gaaggtaaaa ctgctaatcc atatgaatta
     2581 gattgaatat gtaaaattga taatattgct gctcctaaac ctgaagatgc acacataata
     2641 atcaaaggct ttaaattata tcgaagctga atcccaaata aaacaggctc acttatacca
     2701 aaaagttgag ataacattgc tccaaccgca gtttgtttaa cttgattatt tttagatttt
     2761 ttcataaatg ctagacatgt tccaatattc gcaaatccat acattgcaca aagagtaata
     2821 agggcattaa atcctgtatt tgctaatagt gatgtttcaa tcattacata agtatgatgt
     2881 aagccagtaa taacagctaa tggatatgta aatccaataa taaatcctcc aattcctaaa
     2941 ggcaaatcaa tcaataagct gatgatatca accattttta attctattgt atgcattata
     3001 gggcctacac ctaacaaaat aactaacatt gttattagca taacaaaaaa tggtgtgaaa
     3061 ataaggtcta atacattagg catatgttta cggaaccatt tttcaagatt agctccaata
     3121 aatgctgtaa caatagctgt taaaacactt ccttgacaac ctacaatagg aataaatcca
     3181 aatgccataa ttgcttcaat ttcatttgat gggtcagcaa cagaatatgc attaggaagt
     3241 attggggaaa ctaacataag tccaataaca agtccaataa caggtgttcc tccaaatact
     3301 ttaaatgctg accatacaat aattgctggt aaaaaagcaa atgctgtttc tgtaagaact
     3361 gaaacaagtg tctgaatata caatggaata tttgcagaag acattccaaa taatccaagt
     3421 acattatcat taaacaaaca accctttaat cccaagaata atcctgttgc tgcaataact
     3481 ggaataatag gaacaaatat atctgctaga gttctcataa ctttcttaaa tccagtttca
     3541 ttatttttaa ctaattcatc ttgttctttt tttgataaag tttttaatcc cattttttca
     3601 acttctgcat aaactttatt aactattcct gttcctaaaa taatttgata ctgtccagaa
     3661 gtaaagaaaa cccctttaac ctcatcaact ttttcaactt tcttatcatt aataatttct
     3721 ctatctttaa ccatcactct taatcttgtg gcacaatgtg taatagaaat aatattatct
     3781 tcaccaataa ttttaataag ctcatttgct attttattat atttattcat ttttctacct
     3841 cgctttcaat aatactgcat gaaatggttt caaacgcact gttttattta atagatttac
     3901 atcatctaaa ttttggaata caatatcata tttttcttct actcttattt ctacaggttt
     3961 atcagaaaaa ttataataac aatcaataac taccaaatct aattgccttc tatacgcaat
     4021 aacatcttca ctttctatat caataggttc tattgaacca taaatcaaac aatcagaata
     4081 ctcactatct tgtctcaact ttatcatctt tttataaaat tctaaaatac tctctgaatt
     4141 gttaatctga ctctgtgcat taatatcttt ataattttca ataaccttta accatggtgt
     4201 tgtatctgaa aaacctgcat tttctttatc tgcccatgga aatggcgttc ttgcattatc
     4261 tcggcttctt aaattcacaa attctaacgc ttcttcttct gagtaacctt catctattga
     4321 acgatagtat tggtcaatgc tagataaatc attaaaatca tttatggtat ctcttttaaa
     4381 atttgtcatt cctaattcct gcccctgata aataaatggg gttcctctta aaaagaaata
     4441 cattgctccc aacattttta ctgcatcatc attcctttcg tattgcaaaa gatactttgt
     4501 tgtagctctt ggttggtcat gattttcaat aaaattagct gcccatccat gttcctgaac
     4561 atacatttga ctatctaaaa tgcattttct taaatctgga attgtccatg aaattctttt
     4621 aaaccactca ctaccagaag caacatctaa gtcagcatgt ttaaagtcaa agaccattga
     4681 aaagaaacca tcttctccaa taaaatcacc taactggtca tagctaatac ctgctgcttc
     4741 agcaacagtc atacaatcat attttttaaa cgtattttct tttaattcat aaagcatttt
     4801 ttctattcca ggctgatttc taactgtttt tgtacattta acaagaccat caacaccatc
     4861 actttctaaa ctttgaaaac ttaaattctt tttgatgaat gtaatagcat caactctaaa
     4921 tcctgccact cccatttgag accaggtatt aatcatttgg taaagttcac ttcttaatat
     4981 tggattttcc caattaaggt caggttgttt tttaccaaaa gaatgaaaat aatattcgtc
     5041 tctattctct accttttccc aaacacttcc accaaaaaca gacctccagt tatttggtaa
     5101 atcgtttcct tttttaaaaa tataataatc atgataagga ctttctggat tttttaatgc
     5161 ttcttgaaac caaggatgtt catcacttgt atgattaaga actaaatcta atataatttt
     5221 gattcctcta tctttacttt ctttaattaa ctcttttaaa tcttccatac ttccaaattc
     5281 acttgcaata tccaaataat cactaatatc atacccatta tcgtccatag gtgatttaaa
     5341 aattggacat aaccacaatg atgtaatacc taaatttttt aaataatcta aacgagaaat
     5401 aattccctgt aaatctccaa ttccatcatt attactatct tgaaagcttc ttggataaat
     5461 ttgataaacg atttcttttt gccaccattt cttttccata aattactcct ttttatatga
     5521 tatttcttct ttttaattct ttataaattc cattatcgaa tatgtcctca catatagatg
     5581 tcgctatatc tttaagttgt tgatgactat ttttcattgc aattgtaacg tctgatgctt
     5641 gaaacatgac aatgtcattt tgaccatcac caaaacatat ggtttctttt tgagctactc
     5701 ctaatctttc ttgtaatctc ttaattgctt tgcccttatg aaaatctttt tgaataattt
     5761 cataatactg actagaaata tctctctgtg ctaattccat tttatcttgt agtatatctt
     5821 tcacttcgtc aaataccttt tcatttgacc ataaacaaat tttatgtata tcttgtgact
     5881 tatactcttc aatgttattt tcatatgtta ttttttcttg gatatgttgt ttatttatac
     5941 aagaattagt tcctttcaac tgattcattg tttcaaatat ttctttggct ttttgattca
     6001 tgaaaacttt ttcctgactc tcaatcgaaa aagcaacttc tctctttttt aataaacaaa
     6061 caacttcctt tatcaatctt tgattaaaag attgattata aagtaattca ccatgatatt
     6121 gaatgtaatt acctcctcct gcaatatatc catcaactcc taatgataaa acatcatctt
     6181 gtattgtacc catacttctt ccagtacaaa taacaacact gcaatgattc ttttgacata
     6241 aacgtatagc atgctttgca gattctggga ttccatatac ctcatctcta agcgtaccat
     6301 ctatatcaag tattaataat ttgtacaatt tcatcttcct ttcttactat caaaatcata
     6361 atagcattca aaaaaaaaga tatcaatatt ttgatatctt ttaaaaaata tgttcatttt
     6421 tagcacaaat tttcattttc ggggaatatg ttcactttct aaatacatct tgtatttaat
     6481 ataaagcagt tcaattaaaa taaaataagg tgttgttaca tcgtagtcca gttgtttatc
     6541 tacagaaata gatatagatt gaatatacag gttttcatca cataatcttg ctaaaggatt
     6601 acttatcatt tttgtaatag aaaccatata aattcctttc attctcattt ctttagcaaa
     6661 atcaataatt gtagatgcat ctccattcaa tgaaataaag aaaatcatat catctgtttt
     6721 tataaaatga ttaagtgcct gaatcatatc ataaccataa acatcatata taattttccc
     6781 tgttggtaag aaaatccttt ttatctcttt agcaactctt tcctgagaat aaccttctcc
     6841 aaagacgatt atacgatttg cctttttcat tctttcaaac ataatcgaac aatccttctt
     6901 ctctatatca tctatcacct tattataaca atccattaat tgatgtttac tttgattttt
     6961 atattctact tctgtttcat ttaagcgtat aataactctt aattcactat atccaggtaa
     7021 ttgtagtttt tgagcaaaac gagaaagtgc acttgtagat atatggtatt tgtctgcaaa
     7081 ttcatctata gaaagtttaa tacaatcttc tttatgatta agaatacact cacagatata
     7141 cttatcattt gctgtaaatg aattataata tttattgaaa agtccttcaa ttttcattct
     7201 tttctccttc tatactctat gtaattcata tgaagcaaat ctaatagagc ataaaatgct
     7261 cctacgactt cataagatat agcttgtaat gtatcaagtt tttgcgtttc aacatacaga
     7321 ttatactcac aaagtgttgc aattgtatta ttatcaagcc tagttaaaga aatcgtatgt
     7381 atatgattgt tgataaattt tgcaatttca attcctgctt ttgtttctcc agacaaagaa
     7441 atgataataa ataaatcact tttttgaaat gattctctca taaattcaat ttcacctata
     7501 tcaaataagt caataacaca tttacctact gaaagaaata ttctctttat ttcttgagct
     7561 aaacttttct gttcattacc tgtcccataa atataaattg tttctgcatc atatattatt
     7621 ttacaaatat cttgaaatga tattttttgt aattcattca ttaatagtcg ataattctcg
     7681 caaacatcat gaaaattatt atcattatta acaaattcta gttgattatt tttaagtaaa
     7741 tactttaaat cagagaatga atctaatcca atttttctac aaatccttaa taaagtagct
     7801 ctagaaacat tacattcatt tgcaattttt tcacacgaaa aattacggta ttctctttga
     7861 caaatcaata tttgtttaat aataatttta tcattttcat ttaaattatc ataattttgg
     7921 ttaactaacg attctaatct cattttttcc actccctaaa tattatttta atatacatta
     7981 gttgcttttt ctagggaaga gttttaaata ataaggataa aataatcatt tgattttatt
     8041 tttattaaaa taaaaataac ctgctaaaag caggcaccaa aaagtactta tcctttcgaa
     8101 catgtacttt agatattgct ttcttcagaa gcttcatctc ttcatactct aaaatatagt
     8161 tttttctaac tcaaaagtgt tttccatggt agaaattact ttaattcatc tgccatttac
     8221 tttttatctt atttaataat tactattact tgaatattta cttctttaag taaatttcat
     8281 agttaacaaa gctaaaacta ttattataga tgttaaaata caaaattgta agtattgtta
     8341 taaatatgct tacagctaca aatatacatg atatattaaa aagaagctgt aaattattat
     8401 catacataca agcaactacg cctctattaa tccatgttaa tttttattat ttaatacaag
     8461 gagttccatg agacatacat atccattcat attttaaatt atccaattct cttatagatt
     8521 tcatcatctt tttataatcc caattccatg gattaggata aggaactata tttccattct
     8581 tatttttgac taaatcacct gcaaataata catcttcaaa aatcatgcag acatgccctg
     8641 gagtatgacc aggagtatgg ataattttta tattcccaat ctgcatattc tctccataag
     8701 gttcaacatc cttaatgagc cttctggaaa ttgcctttcc aataaacttc ttaaacccag
     8761 gtctatcctt ctctcctata ataaaaggta tatcctctac atgagcccat acttttgctc
     8821 ctgttttttc tttcaataat ttaatattag atatatggtc aacatcatga tgagttaata
     8881 aaatatgctt aatatctgta agtttaatgc caagtgtttt taattcttta attatatttt
     8941 cccccataaa aggaagacct gtgtctataa gaataatttc tttatcaaac acaataaaac
     9001 aatttgagcc acgagtacta tctaacatta atacattttc ttttatcttc ataaataaat
     9061 tccccttatt tggtatttta cctatataat taattaataa agttataaat agttataata
     9121 cttgatatta attttatttt ttattaaagt atactatagg tatacaaata ttatatttac
     9181 ataaatatac catcagtata cttttttatc aatagaaagg aataatttat atgtcagaat
     9241 tatcaaaaag agataaagaa aaaatacaaa gagaaaatga gattattgat aaggcagaaa
     9301 aattattttg tcttaatgga tttgataata caactatgaa tgaattagca aaagaagttg
     9361 aatatactaa acgcaccata tacaagtact tttcatgtaa agaagattta ttctttgctg
     9421 ttgtcttaag aggatataaa agactttggg ataatgttaa aatagaaagt gcaaaaggca
     9481 aaacagggtt tgaaaaaata aaactttcat actttgcttt tcataaattt tactctgtag
     9541 aaccttctct attatgcctt atgggaatga ttggtattgt aaactctgaa aaatcggata
     9601 ctgatatgct gtttaaagaa aagttttttt cttttaacaa atttatgttt gatgaaatac
     9661 aaggaatgtt tgaaattgga aaaaatgata gaagtattag gcatgatatt gaaataccta
     9721 ctcttatgta ttcatcaata tttacactta caggattttt taatttacta tctgtaacag
     9781 gaaaatcata cctgaacaac tttaatatag atgaggaaaa atttattgaa acaactcttg
     9841 ctcttttaat agattcaatt aaagcataaa cctaagttta taaattaaag tttatatact
     9901 taaatgataa ttttttctat ctctaatata aaaaatatta aacataaaac taataactta
     9961 gttaatggaa taactaaatt actgaaagga ttaaaagtaa atggctaaaa tacttctttt
    10021 agaagatgat gatacaatta catttggaat aaaaacttcc cttgaaaaga aaaatcacac
    10081 tgtatgttgt tataatacga ttgctgaagc taaaaaaaat tttgataaaa ctattgattt
    10141 aattctgctt gatttaaatc tacctgatgg tattggatac gagttttgcc aatatgttaa
    10201 aagtatagaa gatactccag tgatattttt gacagtccgt gatgatgaaa aagacattat
    10261 acgtggatta gatatgggtg cagatgacta tgtggttaaa ccgtttttgc tctcagtact
    10321 tcaatccaga attaatgcag ttcttagaag aactcattca aatactaaca atactattac
    10381 atgtgaaaat ataagcttga ataaggttga agtcaaagca tatttagata atgttgaagt
    10441 acctttgaca ccaggagaat ataaacttct tctaagtctt atggaaaata aaaatagagc
    10501 tatgactcgt acaaagttac tagaaatgct ctgggatgta gatggtaatt tcgtaaatga
    10561 taacactctt actgttacaa taaaacgtct tagagaaaaa ttaaaaaatc caccttgtat
    10621 aaaaacatta agaggcatag gatatagaat ggaggacaac tagtgaaaac tccaagaaat
    10681 atcaacagta cagttgtttt tataatattc tgcttaattg taactttttt attgagtgta
    10741 attctaacaa ctataacttc ttttaatgaa tacaatgtac tgtctaaggc tcttggtgca
    10801 tcatatgagt acaatcctaa gtctacagaa agtatactaa aatcactgaa aaattctagt
    10861 gataaggatt ttaaaacagg aaatgaaatt ttatcaaaac atggatattc taaaagtaca
    10921 ttttggagta aaaacttttt ttattttcta gtgatatcgt ttggtataat ttcaacaatg
    10981 gctacagttg tatatataat caaacataga acaaataagc aaaaactgag tagaataaac
    11041 aatcttactc aatacttaga agaagtgaat ttaggaaaag agtctatact tttacgatgt
    11101 gaagatgaat tttctcacct tgaagatgag atatataaaa cagttgggga acttcgttgt
    11161 tcaaaagaat ttgctttaaa agaaagacaa actctttcag ataaccttgc agatattgct
    11221 catcaactta agactccaat tacttctatg tcacttatgg cacaattact ttcagaaaat
    11281 tgtccagatg aagaaataga ttatattaac agacttacaa atcaaatctc aagacttgag
    11341 agactagttt catctttact tacattatca aaattggatg caagtacttt aatatttgaa
    11401 agcaaactta tagatgtaca ttctcttctt acttatgcaa ttgagcctat agaaggttct
    11461 ttaagagaaa aacatcagac atttactatc gaggcttctc cttcggtaga atttgaaatc
    11521 gatattaatt ggactttaga agctctttta aatattctta aaaattgtag tgagcatata
    11581 gaagatggtg gatatatagt tgcttattat agtcaaaatc ctctatatgt tgagattatc
    11641 atagaagata atggtaaggg gtttgctcag gaagaacttc catatatttt taatagattt
    11701 tatcgtggta aaaatgctag taaggacagt attggtatag ggcttgctct atcaaagtct
    11761 attatagaac gtcaaaatgg tactatacat gcagaaaatt gccataatgg tggtgcaaga
    11821 ttcataatta aattttacac ataaaatgct ggattatagg taaaatacct ataatccagc
    11881 tatatctata ttctatgtta tctattgttt tagtaatata cttctattta catttaaggt
    11941 tttattaaac aaacaactta actatcccta tacttgtaag tagtcttaaa tcgaaacctt
    12001 taaataagtt caaatagatt ttgtttatat tgtttcattt tttatagatt ctactatatt
    12061 ttcattcttc aactgcttgt atcctgtcat atatgatgct attacaataa aaagtatcaa
    12121 tatagtaaaa cctattattg tagatatagg tgcaaatggt aaatactcta taaaatatat
    12181 ttcatttata cgtagaaaag catacactat tcctatttgc acaggaatac taagtagtaa
    12241 tggtgtaatt cctaagaata aaccttccaa aataagcatc ttttttatgc cttctggaga
    12301 taatccaaca gaacgcaaca tagcaaactc ttgtcgtcta cttctaatat ttcctgacac
    12361 tgtagcaagt acatttgata atccaatcat agccaataaa cctgtaataa agcctactat
    12421 taaattcatg gtctttattc cactagcctt ttgtttttca ctttctaaaa catcctcaat
    12481 actataatct cctgaaccat agtctttatt ataaatggac tctaattttt ctcttactct
    12541 aggaatgtcc tcacgattat ttactttaaa tactcctatc agcctatttg aacgtaacct
    12601 acttacttca tcataatttt tagatatttc ctgcaccata gacattggaa ctatttgcat
    12661 taatgtatac tttgaactag tatctactat tttaggaagt gtgcttgtaa tagctcccac
    12721 tttcatgttg taagtataat cccctttatc ttcgtcatat actttttcat ttaatttaat
    12781 gttatctcct attgaaagat ttaaataatc tatatatact ttatttctac gtgtactacg
    12841 attaatatct tcctccttat tatatacaat tgaggttgga ttttttgtat cataaaataa
    12901 ctttgggtct acagaaattt gcctacaata ttctgtaaaa ctcttatcat caagcccaat
    12961 tatatttgaa attatacgaa acttgccatc acgttctata ggagagtact ttttagatga
    13021 agctatattt ttaaatcctc catttttcgc aagttctttt gaagcatctt tttcagtcaa
    13081 ccaagtggaa gcagacattt tttgataata tagtgaactt tttacacctt ctgtattatc
    13141 taatttttca atgaaagcag actctacagg ttcactattt tccaaataaa ctgctatatc
    13201 tctttggtcc ttataattat cacttccata aacagctttt gctccttctt gatttgcaat
    13261 tatatgcaaa aaacatgtaa gtaataaaaa agataatgtt aaagatattg ttgcagtacg
    13321 ataagatttt ttacgagcac gtagtgcgtt tgatgcaagt tctccttcta ttccaaatat
    13381 ctttcctaat gaatatttat gcgacttttt gattttaaca ctatctcctt gtcgtattgc
    13441 ctctatagga gatatttttg atacttttcg tgctggtata agtgcagaaa tccagactgt
    13501 tactattgta agtaaaactg ctggcagaaa agcaggaata ccataagtaa ataccacttc
    13561 tggagcatca gcatgataat tacctgaatt aatatagact ataagcctat tacacaataa
    13621 ccatcctaaa aataaaccta ttggtaatgg tataattgtc agtaaaaatc cttcaaacac
    13681 tacagacctt ttaatttgtt ttggtgatgc accaacacta gataatatcc ctaactgagt
    13741 aagtctagca tttgcagaca gtgaaaacgc gttgtaaatc accattacaa acacggctac
    13801 tgtaaaaatt gctattgtaa gatacataat aggagttgaa aattttgata tactactcat
    13861 agatgctttc tgttctggtg aaaagactaa catctttaaa agataattac tattgtattt
    13921 aagattgtat tttccatatt cattagtctt ccatcccaat gatttagcaa gttttggaag
    13981 ttccttatac gtgtctctta tatttttaaa tcgcaagtac acagttatac tatcattagg
    14041 ttttatactt gttttatcaa gatatccaag agctgtatat gcagatatta ctgatgaagt
    14101 agaaacatct acctttccta ctattgtcaa tgtagcctct gaaatttttt taaaactttc
    14161 ccctttcgat tttggggcta tgggttcaag aggctttcca tttaggattc tatctcctat
    14221 tgggagagta attttatcac caatttttac acttggatta ttctcaaaat attgttttga
    14281 taatgcaatt tcattagcat tatttggtcg acgaccctct attatagtat ccttttctgg
    14341 catagaattc caatactctg atgtagcatt tctataaatt atataatctc ttttctcatc
    14401 attaattttg cctacatacc attgtccttt taccattaca tcatcaactg aagaaaaatt
    14461 ttcaattaat ggtaagtcac ttccataagt atagtcaaac aattcaccat gccagttacc
    14521 ttctttctca attgaaagtt ttataaaatc tgtccacatg gtatacaaca gtccacacaa
    14581 agaagacatc atggttgtag taagtaatat agctaacatt atagctatac tactacgttt
    14641 attgcgtcgt acaaagtcta aggtgtattc ttgaagaata ttcatttctt caccaccttt
    14701 tcagagacaa tcactccatc ttccatagtt acaatacgat ttgcctctag tgctattttc
    14761 tcatcatgag taataagaag tattgtctga ttaaatcgtt tatttgataa gtttaacaag
    14821 tctacaattt cttctgaatt ttttctatca agattaccag ttggctcatc tgctaataaa
    14881 atagctggtc tatagattaa acttctagct atagataccc tttgttgttg tccacctgaa
    14941 agttgacttg gaagatggtt taatctatca gataatccca atatagatac aatctcagag
    15001 aatctatctt catccacctt tcttttatca agtaacatgg gtaataatat attttttcta
    15061 acatcaagag taggtataag attataaaat tgataaataa gacctacttt tctacgtcta
    15121 aaaactgcaa gagcttcttc ctttaaactt gatatttctg tatcttcaac ataaatcttt
    15181 ccagatgtag gtctatctac tcccccaagc agatgtaaca atgtagattt gccagaacca
    15241 gatgctccta caatggatac aaaatcacct ttttgaactg ataaatttac attattaagg
    15301 gctgtcactc tggtttgatt tgaaccatat atttttgtga gattttcaca ttttaatatt
    15361 tccatataca atcatctcct ttatggttaa attttagcaa cctaaaatga cattatagtg
    15421 acatgaatac aaatataaat tactattaaa aatattgttt agtttttgca cattatttaa
    15481 aattataata taaaacaaaa actatgcaat atattttatt tatacttt
//
LOCUS       DAEMIK010000103        15518 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEMIK010000103 DAEMIK010000000
VERSION     DAEMIK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.103"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            423..1853
                     /locus_tag="KPK87_002636"
     CDS             423..1853
                     /locus_tag="KPK87_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2538777.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1956..3059
                     /locus_tag="KPK87_002637"
     CDS             1956..3059
                     /locus_tag="KPK87_002637"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG2538778.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            3074..3310
                     /locus_tag="KPK87_002638"
     CDS             3074..3310
                     /locus_tag="KPK87_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2538779.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3327..4376
                     /gene="vorB"
                     /locus_tag="KPK87_002639"
     CDS             3327..4376
                     /gene="vorB"
                     /locus_tag="KPK87_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2538780.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4378..5118
                     /locus_tag="KPK87_002640"
     CDS             4378..5118
                     /locus_tag="KPK87_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2538781.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5121..5660
                     /locus_tag="KPK87_002641"
     CDS             5121..5660
                     /locus_tag="KPK87_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2538782.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5784..7883
                     /locus_tag="KPK87_002642"
     CDS             5784..7883
                     /locus_tag="KPK87_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG2538783.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKIDSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8150..8665)
                     /locus_tag="KPK87_002643"
     CDS             complement(8150..8665)
                     /locus_tag="KPK87_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG2538784.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8757..9968)
                     /locus_tag="KPK87_002644"
     CDS             complement(8757..9968)
                     /locus_tag="KPK87_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG2538785.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10644..12086
                     /locus_tag="KPK87_002645"
     CDS             10644..12086
                     /locus_tag="KPK87_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2538786.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12128..12481
                     /locus_tag="KPK87_002646"
     CDS             12128..12481
                     /locus_tag="KPK87_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538787.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12672..13493
                     /locus_tag="KPK87_002647"
     CDS             12672..13493
                     /locus_tag="KPK87_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG2538788.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            13751..>15518
                     /locus_tag="KPK87_002648"
     CDS             13751..>15518
                     /locus_tag="KPK87_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG2538789.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNII"
BASE COUNT         5982 a         1753 c         2813 g         4970 t
ORIGIN      
        1 taaatcaata gtacatgtat aaaaataaaa ggaatttaaa taagaaaaag atatttttaa
       61 tatctggatg tagtatagaa atatcttgaa taaattattc ttgaataaaa gtataaaaaa
      121 ttaaaatcac tagaaatatg ataaaataat ttaaaatacg catttcaatg acatttcaaa
      181 acaagaaaat aataaaaaac aaacctaaat taagtctaaa tagcttttta gttaatttta
      241 gtagtaaaaa ataaaactta aaaagtgttt gacaattatg aattttgaga atataattaa
      301 attaaaatta tacacatata aaaatatgct ctgaatcaag aatgagatat ttaagattca
      361 aaatatatat tattatacgt gaataataaa aagtaagaaa attttataag ggaggggtag
      421 atgtgagtag tcaagaaagc aaaccaaaag gaaatcttat atcaaatttt agagatgata
      481 tgagagaccc taagaaaaga gcaatataca catattcaag ttctataaac ttagcgtaca
      541 attttatagg ttactttgta tcaactgtta caaatatttt tttaactgat gtggcaaaac
      601 ttggtgtaat tatggcaggt ggtatgcaaa ttatacaatc tttcataaaa attgctgtag
      661 ctcctataac aggtacagta tttgacaaac aaccattcaa aaaaggaaag tattatccat
      721 ggcttagata tgcaccagca gcgttagcat taacatatgg aatatttttc atagttccat
      781 taatgaaaat aagtccagca ttacttttac ctttgatgac agctttatta ttaatagcat
      841 cttgtgctca gcaagtagtt ttcactataa tttatgctat ttaccctgta gttgctaaga
      901 cacctagaga ccgagttata ggctcaacta ctacgaatat atttaaagaa gttggtaaat
      961 tcttagtagg tttttcatat ccgttattat tagttttatt tgcagatgta ttaggtggag
     1021 aaggaatggg ttatttaggt acctacttat tctttgcagc aggatgcctt ttagtatttt
     1081 ggttttcttc aacagagata ataaaatctg gagcagaaga agaagttctt caaaaagaag
     1141 agaagccaaa gattaaagca agtgaaatgg caaaagcttt attcactaat gtaccactta
     1201 tgatggcatt ttgtttagaa tttcttatat gtataagatc tataggatta ggacctttgg
     1261 ctccatatta ttttaaatat gtaatagaag atgaacgtgg attagcagta tttttatcaa
     1321 tcatgccttt ggtaagtgta gcattcatgt ttttcgcacc agtgtttata aaaatctgta
     1381 gagagcaaaa actagcttca ataatttctt tttctatatg tgcactatgc cacttattgg
     1441 ttgcatggac tccttggggg aaaactactt taggagtaac tatgttgttg gcaattggtg
     1501 gaggtttttc aaatgtagta tcaataataa atcttaattt ctttgccggt tcttgtgatt
     1561 atgggcattg gaaatcagga aaagacttac ctggcttaag tatgtcacta tatccagttg
     1621 caatacaagt aggagtatta cttgcaacta ctataagaac agttttaatg aactcaatgg
     1681 gatatcaagc tgatatggta gtcacagaag cagttaaatc aggttttata aatatgattt
     1741 cttattctat ggcaatacct ttaataatag ctgttgttat agcaatacta tatccagtaa
     1801 gtgacaagaa attaaaccaa ataagagaag agttaaatca aagaaatgct tagttaaaag
     1861 aaagttgagt agatggttaa tttaagctat taaagtaagt agacgcaata gcgtttatat
     1921 aaatgaaaat acatatacag agggggcttt taaaaatgat agaaaaatta ttgagaaata
     1981 ccgtaaaaga attacatcaa tatgttccag gtgaaccaat agaaaaagtt aaggaaaaat
     2041 atggtgtaaa agaaataata aaattagctt caaatgaaaa tcctctagga ccatcaccaa
     2101 aagcaataga agctatgatt gaaatgttaa aacaaggtca actttatcca gagccagaag
     2161 ctaacgaact tagaagaaaa ttagctgaaa agctagattt aaaaccagaa aattttattg
     2221 ttgctaatgg tgcagataac gttatcactc taataggtga ggcttttata aatagaggag
     2281 atgaagttat atattgtaac ccaacatttc catcatatag aactgcaact ataaaaaatg
     2341 aagggatacc agtagaagta cctttaacag aagattataa gtatgattta caaggtatat
     2401 tagataagat tacagataaa acaaaattaa tatgtgtatg taaccctaat aatccaacag
     2461 gaacaattgt ggatgataaa gagttagaag aatttttaaa gaaagtccct gaaaatatta
     2521 taactatatt ggatgaagca tatatagaat ttttaacagt tcctaattat gtggatggac
     2581 ttaagtatgt aagggaaaat tataacgtta tagtaacaag aactttctct aaaatatatg
     2641 gtcttgcagg gcttagagta ggatatggta ttgctaaaga tgagataata agaactttat
     2701 ttacagtaaa agagcctttc tctgcaaatc gtgttgctat aagtggagca acagcagcac
     2761 ttgatgatga agagtttata aaagaaacat atgaattaaa tagagttgga atggcttact
     2821 ttaaggaaga gtttacaaaa atgggatttg atgttgtaga ttcacaatca aatttccttt
     2881 atgtagatat gaaaacagat atacctaagt tatttgaaga cttgaaaaaa agagggtttg
     2941 taataagacc aagtgctaca catgcacgtg taagtattgg aactatggaa gaaaataaga
     3001 aatttgtagc agttttaaag gaaatattaa atattaaagg agagccaagt attggataat
     3061 agatagaaat attatgaaac ggggtgtaga tatgaactta aaaataaacg cagagagatg
     3121 taaaagttgt gaatactgcg ttatatcttg taaaaaagga gcattaaaaa tatcaagtaa
     3181 gataaataaa gaaggttatg ctcatgtaga agttgatgag agtaaatgca tactttgtgg
     3241 aatttgctat caggtgtgtc cagataatgt ttttgaaata attaaagaag aagcatgtca
     3301 agaggcttag aaggagttgg gataatatga aaaagatgtg gaaggggaac cacgccattg
     3361 cagaagcagc cctaagagga ggttgtgaat tttatgcagg atatccaatt acaccacaaa
     3421 cagaggtaat ggaattttta tcacatagaa tgtctgaact tggaagaact tttatacagt
     3481 ccgaaaatga gatggctgct atatatatgg tatatggagc atatgcaagt ggaatgaggt
     3541 ctatgacgag ttcttcagga ccaggaatat ctttaaaaca agaagggatt tcttacttat
     3601 gtgcaaatca ttacccttgt gtaatcgtaa atgtacagag atgggggcct ggtcttggct
     3661 cattggactc agcacagaca gactatctaa gagatactag agggggagga aatggagatt
     3721 atcatttgat agtttatgca ccaaacagta ttcaagagac tgtagatttg atgtataact
     3781 catttgatgt agcggagaag tacagagtgc ctgttgaaat tttgacagaa gcagctttag
     3841 gtcaaatgat ggaaccagta gaatttccag agtttaagaa gagagaagga gatttaggtt
     3901 ggacttatga tggaagtaat agagaccatg caaaggttgc agataaccaa aagccaactt
     3961 tttgtatgga gaaacgtatg agaataactg aaaatgaaca acaatgggaa gattatcaag
     4021 tagaagatgc agaatatgtg tttgtagcgt tcggtatacc atcaagaaca actatgaatg
     4081 ctgttagaag acttagagaa caaggtgaaa aagttggaat aataagacca ataacagtat
     4141 ggcctttccc atataaagca tttgaaaagg taagtaaaga tgtaaaagga tttatctcag
     4201 tagaatcaac agacacaggt caattagtag aagatgttgc tttagcctca aagaaagtat
     4261 gtaaagaaaa tgtacctgtg tatggtttat ttagtggaaa tcatatacca aaaacattgc
     4321 aagtaatgga cacttatagc aaaataaaat ctggcgaaat caaggaggtg ttctaatatg
     4381 gctacagaaa aattaacacc tagcgttgtt tcaataccaa acaagttttg cccaggttgt
     4441 ggtcatggaa tagtaaatcg tataatagca gaggtaatag aagaacatgg atatgaaaaa
     4501 aatcatgtat taacattagg agttggttgt gtctgcaata tgaattttag ctggaatggt
     4561 gataagatgc aaactgcaca tggtagagct tcatctacag ctataggagt taaagtagca
     4621 cttccagata cattagtaat gacttaccaa ggtgatggag atgcttatgt tataggtctt
     4681 tctgaaactt taaatacagc atatagaaat cataatgtga ctgtatttgt aataaacaat
     4741 aataattttg caatgactgg tggacaaatg tcatggacca ctatgccggg tcaagtcaca
     4801 acaacaagtg taaatggaag agatataaag actactggta gtccactgaa agttcctgag
     4861 atggtggcaa acttctttga tgtagcatat gtagcaagag gttcagttca tagcccaaag
     4921 gaaataatta atctaaaaaa atatataaaa aatgcaatag aagctcaatt aaatggagag
     4981 ggatattcct tagttgaaat attagcacct tgcccaacta actggggtgt atcactagag
     5041 aaatctatca aatggatgga agaagaaata gtgccatatt atgctcttgg cgaatttaag
     5101 caaagggatg gtgaatagtt atggaaaaag agctagtatt tgcagggttt ggtggtcaag
     5161 gagttttaac tcttggtctg attgtagctg agtcagcact tgaattgggg aaacaagtaa
     5221 cttggatgcc tgcatatggt ccaactatga gaggtggaaa agcctatagt gtagtaaagt
     5281 tttctgatga gagtataggt gggcctgata tggaagaaat agatgtttta gtagcaatga
     5341 ataaaccatc tttagattat ataaatttag ttaaagaagg tggaacagta gttattaata
     5401 catctgcaat agatgagaat gtaacattaa gagaagatat aagtgtagtt aaaataaatt
     5461 gtcaggaatt agctcaaaaa gtaaacaatc caaaagcagc aaatatagta gtacttggag
     5521 cattaattgc taaaactggg ttatttgaaa aagaattagc tttaaaaact atgtgtgatt
     5581 tctttgagga aaaaggaaaa ggaaagttta atgttcaaaa tgaagcagca ttcatggaag
     5641 gctataataa cgccatttga taaattaccc ttatcttaag tgatttatct caagatttaa
     5701 taatctttag tgccaagaga aacttcatct tgagataaaa ttcacttaaa atcacaacta
     5761 taaacatata ggagcgtgaa tttatgagtt tggaaaaact tattagacca aaaagtattg
     5821 ctatagtagg agtgactgat aaacttggtt ttggaagaag tgcagcactt agtatagtaa
     5881 aaagtaagga aacagataga gtatattatg ttaatcccaa aagagaagaa ttgtttggga
     5941 gaaaatgtta taagacgata caagaagttc cagaagtagt agactgtgta gtagtatgta
     6001 cgcctagaaa tgtagttcca tctgtattaa aagattctgg agagttagga acaaaggcag
     6061 cagtggtata tgctagtgga tttgcagagg aaggtacaga ggaaggtaca gatttagaaa
     6121 atcaacttat agaaatatca aatacatacg atatgaaaat attgggtcca aactgcatgg
     6181 ggttgctaaa ttgtatagat aaggttaatc tatgggcagg aggttctaaa tgggatttag
     6241 atactaaaaa acctggtata ggaattgtag gtcaaagtgg atttataact gctgaaattg
     6301 tttcaagtga ttattttaat atatcttatg gtttttcaac aggaaatgga aatatagtta
     6361 cactagaaga tgcagtagat ttccttgtag atgatgatta tgcatcagta atagcaattt
     6421 accttgaagg tttaaaaaat cctcaaaaat ttatagatgc tttaaaaagg gcagcacaaa
     6481 aaagaaagcc agtaataata ttgaaatcag gaaggtcaga aaaaggtgct atttctgctg
     6541 cttctcatac aggaaattta gctggttcga gtaaagcttt tgaaagtatt tttgaaaaat
     6601 atggagtaat ttctgtggaa aatttagaac agtttatgtg tttagcacaa gcttttagtg
     6661 ttttagatgg taatttacct gcaaattcta attttgcagc aataaacttc tcaggaggag
     6721 aaaatactat ttgtgcagat ttagcagaag aaaatggtgt agaacttgca gaaatatctt
     6781 ctgaaactaa agaagaaatg aaaaaatatc ttccaggatt tgcaacacct aaaaatccac
     6841 tagatgctac aacagcatta ttccatgaaa aggatatgat agtaggttta ttacatacct
     6901 ttgaaaagga tgatagtgtt ggatttacta tggtaggtgc aaatataaga gatgaagaaa
     6961 atgaaatgca tgaaaccctt tgtaaagctg taagtgaagc tagagaacaa ggattgaaaa
     7021 aacctgtgtt tgcagttcct acacttgaag gaaatagata tttagattat aggactagat
     7081 tagaagataa tcaagtgcct ataatgtcat cagttggaac tacatttact tgttttaata
     7141 aaatggctaa gtttatagat tatgattatt caaaaagaac tttagaattt aaagctgtta
     7201 agaaaagaga gtcaaataat gtagtagcat tatcagaatt agatagtaag attgagatga
     7261 aaaaatatgg agtaccagtt ccaggacaag gtaatgcaaa aagtattgat gaattagatg
     7321 aaatcttaaa atatattaaa tatcctgttg ttttaaaaat agattcttct gaaatattgc
     7381 acaaatctga tgttggagga gttaaaatag gaattaagaa tagagatgag gcagtagatg
     7441 catataatga gattttaact aatgtaaaaa aagcaaaacc agatgctaat attgatggta
     7501 tattagttca agaaatggta gaaagtggta tagaaattat tattggtata actaatgatg
     7561 accaatttgg accaatgtta ttagtcggtt taggaggagt atttgtagag gtatttaaag
     7621 atactacatt atatccatta cctataaatc atgatgaagc tattatgatg cttaaaaaac
     7681 taaaatcatt taagctttta aatggctata gaggaagtga gccttgcgat attgatgctc
     7741 ttgcagatat gatggtcaaa ttaggaaaat atgcttatga aaataaggat gaagtcaagg
     7801 aaatagactt aaatccagtg tttgtatatc ctaaaggaaa aggcgtatgt gcagtagatg
     7861 ccttaatagt taaatataaa tagtttataa gttttggtat tcaaccataa ttaaagcacc
     7921 gactgaaaat cacattttgt aagttagata gtgtgatttt cagtcacccc aaatttaatt
     7981 taaaaatatt ttaaaatgaa agtatagttt taagtttcat tgtgaaatag cactgtataa
     8041 acaataaaat actacgtata aaacgaataa taaaaggtgt tttaatgata agaatcaatc
     8101 ttgattttca ttaaaacacc ttctttatta tattaaaaat ttttaaacgt tatattgtca
     8161 caggtgggtt tgttggagct gcatatgtac gattagcaaa ttcttggtca acaaagaata
     8221 gaccaccctc tccagcaagt ttaacacgtt ttatattgtc ttcacaattt gtctcttctt
     8281 ctgcttgttc agatataaac caaagtaaga attgagaagt tctaaagtct gattcagagt
     8341 ttgctacagc tgctaagttg tttattaaag ctgtaacctt tttttcatga gctaacgtac
     8401 gctcaagaat atccattgca gaagtaaagt ctgaatctgg cattgggata gcatcaagct
     8461 gaacctttcc tcctacatta tgtaagtatt tgtaaaaaaa catagcgtgg tctgtctctt
     8521 ctttatattg aacataaaac caattagaaa atcctttaag tccttctgct tcaaggtaag
     8581 aagacataga aagataaaga tacgcagaat ataattcatg gttaatctgc tcattaagta
     8641 atttttccat tttagctgaa atcattttta tacctccaaa ttttaaatgc tcttataaaa
     8701 tataataata ttaaatggaa aaatattcaa taaaagtgtt taaatatttt acatatttaa
     8761 taaaaattta tactaccatt aaaaaaatta taacttacaa tggctataga gccaattaat
     8821 ggtgcatttg ctccaaaatt tgaatgcctt acacttattt ttttatattt agaaaaactt
     8881 gctgaagcag tcaatttttt taataatgta ttttctataa atgtaccagg tatattaaag
     8941 tcatagccta ttattataga actacaatca atcaatgtaa gtgtattaac taatgcatat
     9001 gatacgtatg tacaaaattc atcaagcaaa aacatggcaa gactatcttt attatttgcc
     9061 tcatctatta acttgactaa agaaaaatct ttacagttaa ttaatgggga gttaggataa
     9121 atatggctca atgattcaat tcttttaatt agattactga cacttgtata ataatctaaa
     9181 catccattgt taccacaact gcacaatggt ccactaaaat taattgatgt atgtcctatt
     9241 tctccacttt gtcctaggtt gccagtatga agtttatttt ctagaactag accagctcca
     9301 ataccattca ttatgtgtag gtatatataa tttgatatat ttttacctaa accatacatt
     9361 ttttctgcta atgcacctga attagcatca tttatcaaaa atattggcaa atcagacatt
     9421 tcttttataa aatctactat atttaggttt gatacatttc caaaatctgg gggatttagt
     9481 atggtaccat ttatatcatc tactggacct attgaagaaa taccaattcc aattatatct
     9541 ctcttgttga ttttcattat tttagtaaac attcttttta ttatatcttt taaaacatca
     9601 tttgatagac atttggggta agtttcagaa agttgtttta caatattacc ttttaaatct
     9661 gaaataatta ccttacatat atttcttctt attaggattc cacaaataca aggcgatata
     9721 ttagaaatat ctaataaaat aggtttacga cctgatgaat gtgcatttaa agttgatgac
     9781 tctatttccc taatcatatt ttcattaata agttcagtaa ctagatttga taaagtcatt
     9841 ttagtaagtc ctgtagactt agctaaatca actctggaca taggtgtatt tgtagaaagt
     9901 aatttcaata ttattaactt atttttaatt tttacatctt tgatgttaga accttgtttg
     9961 ttactcatta taaatctcct taaataaagt taagtgtttt atttattttc aagtatacta
    10021 tcaccttata atagagtaac atttttttac ttgaaaatat cattaattag aatatatcac
    10081 attatatatc ataaaatata taaaaaaatg ttagaattaa attaaaagtg aaaagtagat
    10141 tgatatataa atagtaaata cttaattaaa aaaagataat gatagcaata attattgtta
    10201 ccaagaatct actacttcaa gtgttacctt aggtatttaa gttgatagtg gtttgcacgt
    10261 aataaacttg atgtaatata tattcttaat aatattgata aaagatttac ttaaaaataa
    10321 tatagattat gtaaacatat aatctattta ttgtatacaa taaaatttca ttttgtaaaa
    10381 cagtgaatta aatatgtaat atagatatcg atttttatta caaattgtta ctaaatatag
    10441 gtacattaga acaaaaatct agtaaattgt aactaaaatg taaatttttc ctttaatcta
    10501 tacaatatta tggaaacttt ttgtaattta tgatataata acaaatatat aattattaaa
    10561 ttataataaa tgactatagc gtagatgtaa tactatgtta tagtcatttt tgaattaaac
    10621 ttgatatatc aagggggaag aatatgagaa caattagaag agttttagca ttaggattaa
    10681 cattagctat atttctaata aatgtaccta atgtagatgc attaacatca gatacaatta
    10741 aaggtaacaa tatatatgag acggctggat taatagcaga taaaaagagt tatgatacag
    10801 caattatggt aaatatggat aattctatag cagatggtct ttcagcaagt ggtcttgcag
    10861 gtgctgttga tgctccaatt ttgcttgcac aaaaaaataa gataccaaat gaaacaaaac
    10921 aaagattaaa aaatgttaaa aagatataca taataggtaa agaattatca ataagtaagt
    10981 cagtagaaac tgaactaaaa aacacagggg cacaggtaac tagattgggt ggagatgaca
    11041 gaataaagac tagttatagt gttgctaaag aggtaaatgg tatcaaaaaa gttgatgaag
    11101 tgatattaac taatgcatat aaaggagaag cagatacaat aagtgctgca cccgtatcag
    11161 taagagatat agctcctata gtacttacag atggaaagag tgtgcctttt tcaacaagca
    11221 atgtaaaaac atatgcagta ggtgggagta tatcaatgag cactagttta gttaataaaa
    11281 ctaatgctaa aagacttggt ggttcagata gatatgatac aaataagaaa gttataaaag
    11341 agttttatcc agatgcatca gaattttatt tgagtgatgg atatgattta gtaaatgcac
    11401 ttacaggttc tacaattgct aaggaaaatc caattgtatt ggtgtcagaa agtagtgata
    11461 agtctatatt agcaggagca gataagatta ctagattagg ttcaataagt gacagtgtgt
    11521 ataataagtg tgtttctgct gcacaaaata atggcgattc gtctacaaaa ggtgagtcac
    11581 ctatgaaaaa tgagacaagc atattaggtc agccaacagc tagtttagaa gcgtgcttaa
    11641 aatgggcaaa atctaaaaaa gctaatgatt tatttataga gttaatacca atattatatg
    11701 atactgctgt tcaggaaggt gttaatcctg ttttggcagt agctcaatct gcaaaagaaa
    11761 ctggtttttg taattttggt ggagtattag atgcatcatt taagaatcct tgtggactta
    11821 aaacttctgt aggtggttct gatactgata agaatgccca ttcaagattt gatacttggg
    11881 aagaaggaat attagctcaa attcaacatt tatgcttata tgcaggacaa gatggttacc
    11941 cactttcaaa tccagtagac cctagacatg aaaaatcttt atttggtaag gctaaaacag
    12001 ttgaaagttt atctaataat tgggctggag gtcaatatgg acaagattta gtaagaatga
    12061 tgggagaaat tgaggcaaca aaataattga agattatcaa atatccaata aataaggatt
    12121 gattattatg aaaaagagta atatttttag tatcatattt cctacagtca taatggtgct
    12181 aatgttttta acttttgcta cagatatatt taatattcca gatattcaat ctaaaagtat
    12241 ttttgttgtg ggaatggttt tgatttttcc aatttctttt ttgatacaag ggataatttg
    12301 tgtattaaat aagactaatt ggattttatc tcttattgtg tctttagtaa cttatatagt
    12361 attaatgtat atatttttaa atgattctgc atatatatat gttttacttt atacagcttt
    12421 ttatatgatt ggacatataa cagctaaact ctattacaaa attaagactt ttaaaaatta
    12481 aattgaagta tataaaagta acattaggag aagtatcctt aatgattttc taaaatcaga
    12541 aataggtgct tctctttttt gtaaaaataa aagtatgtaa ataaaattta accattataa
    12601 ttttctttta acaaatgtat acaataaact tgttgtataa ttaagataga taaaataaag
    12661 ggagggataa tatgaattta atagataatc atattcatac taatttttca agtgatggaa
    12721 aggattccat ggaagataca ataaaaaaag ctataagtat tggtgttaga tatttgactt
    12781 ttacagacca tttagaacat gatgaagata gaggattttg cataaattat aatgactatg
    12841 ttccagtatt taataagttt aaagaaaaat ataaaaagga catagagtta ttactaggtg
    12901 ttgaagtggg ttacagaaaa catctaaaaa atgaaataga agaaataata aattctaatc
    12961 cttttgattt tgtattgtgc tcaactcata caattgacaa tgtacctgtt ccatcaaagg
    13021 agtattttaa aggtcttagc aaagaagatg cgtattataa atattttaat agcatacttg
    13081 aaactaatcg tgaatttgga gattacaata tatatggtca tttggattat atatctaggt
    13141 atggaatata ttcagataat agagttatat acaatgactt taaagatgtt attgatgagg
    13201 tcttaaagtc aataatcaat aatgggagtg gtatagagct aaatacttct ggttatagat
    13261 atggactaaa tgctatacat ccaaatgaag atattctaaa aagatataaa gaacttggag
    13321 gatttatagt tactgtaggt tctgattctc atagggtaga agatatatgc aaagattttg
    13381 atgtggccta tgatatgctt aagtatcttg actttaaata tgtatcttta tttaaagaaa
    13441 gagaaactta ttttgtaaat atagaaaaag ttaaatctaa caatatagct tagagtgtgt
    13501 tttgaagtaa attaatggat agttaaaaag ttaatagtta taatattgta tcctctagaa
    13561 aaatactaga ggtttttatt ttgctaaaat aactaaatag aattaaattt aaatagtata
    13621 gtttatttta aattgaatag tttattgttg atatatttta ctattatata tttttattgt
    13681 gatataatag taaaatatat taacctatct ttagattata taaattatta taaaggaggg
    13741 aaattttaaa atgtctaata aaaataaaga aaatataact ttaagtaatc aaatcaaagg
    13801 aacaattcaa aatagtttaa caaaagaagc tatattacat ataccaatga gcaattatgc
    13861 ctatggttat gatagagaaa cactacatat aagacttaga actaaaaaaa atgaagcgaa
    13921 aagagtaatt ttacgaatag gagaccaata tgtgtgggat aaaggcggtg caggaggagg
    13981 aaatttaaat gcatctggag ttggttggtc tggtggaaca aatatagtaa tgagtaaaga
    14041 agtagaaact gaattatttg actattggat agctaagtgt aagcctttaa ataaacgctc
    14101 acgatatggc tttataatag aaggtcaaga ggaaaaactt ttatttacag aaaaaagaat
    14161 aatagagttg ggaaatgaaa atgatgaaaa ggaattatgt gaaataggta atttttttgg
    14221 atatccatat ttaaattata tagatatacc taaagtgcca aattgggtga aagatactgt
    14281 ttggtatcag atatttccag atagatttgc aaatggtgac ccgtctataa acccagaagg
    14341 agtagataaa tggggagcta ttccaaccag agataatttt acaggtggag atttacaagg
    14401 tgtgatagaa cacttagatt atttgagtga tttgggaata aacggaattt acttttgccc
    14461 tattacaatt ggaaaaacca atcatagata tgatacggta gattatatgg aggtagaccc
    14521 tacattagga gataaagaga ctttaaaaaa actaatagaa gaagcgcata agagaaatat
    14581 aaaaataatg ttggatgctg tttttaatca tataggatat tattctaaac agtggcaaga
    14641 tgtagttcaa aataaggaaa attctcgata taaaaactgg ttttatataa aagatatgag
    14701 taaaattgat actccaatag aacaaataga tgaaaaaaat ataccttatg aaacctttgg
    14761 gtgtgaaaaa tatatgccta aacttaatac agaaaattca gaagtaataa aatatttatt
    14821 ggatgtaggc aagtactgga tacaggaatt tgatatagat gcgtggagat tagatgtttc
    14881 aaatgaagta gaccatgttt tttggagaaa gtttagagaa gaagtcaaaa aagtaaaacc
    14941 agacatttat atattgggag aaatttggca tggtagttta ccatggctaa tgggtgacca
    15001 atttgattca gttatgaatt acttgatgtc agaagccatg aaaaaattct tttgtacaga
    15061 tgagataaac gccgaagaat ttaagtatat gataaatgat gtaatggtaa gttatccaat
    15121 acaagttagt gaagttattt ttaatttact tggaagtcac gatactacaa gaatattgac
    15181 ttatgctaat ggaaatattg atagatttaa acttgcatat atgtttatgt ttgtacaaac
    15241 aggatgtcct tgtatatact atggtgatga aatcggaatg gaaggagaat taagttcaat
    15301 atctgaaggt caaagaaaat gtatggaatg gaatgaagaa aaatggaata aggatatatt
    15361 tgattttatg aagaagatta ttagacttcg taaagaaaat aaagaactta ggtctatatc
    15421 aaatgaatgg gtgttagcag ataaggaaaa tggaacgatt atattgaaaa aagaatctat
    15481 ttctataata ataaataatt catctaaaaa tataattt
//
LOCUS       DAEMIK010000104        15458 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEMIK010000104 DAEMIK010000000
VERSION     DAEMIK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.104"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(153..884)
                     /locus_tag="KPK87_002649"
     CDS             complement(153..884)
                     /locus_tag="KPK87_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2538790.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            complement(1161..2467)
                     /gene="grdB"
                     /locus_tag="KPK87_002650"
                     /pseudo
     CDS             complement(1161..2467)
                     /gene="grdB"
                     /locus_tag="KPK87_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
     gene            complement(2553..3839)
                     /locus_tag="KPK87_002651"
     CDS             complement(2553..3839)
                     /locus_tag="KPK87_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG2538791.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(4118..5440)
                     /locus_tag="KPK87_002652"
     CDS             complement(4118..5440)
                     /locus_tag="KPK87_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG2538792.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRS
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(5440..7434)
                     /locus_tag="KPK87_002653"
     CDS             complement(5440..7434)
                     /locus_tag="KPK87_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2538793.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDVVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(7521..8768)
                     /locus_tag="KPK87_002654"
     CDS             complement(7521..8768)
                     /locus_tag="KPK87_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2538794.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8996..9541
                     /locus_tag="KPK87_002655"
     CDS             8996..9541
                     /locus_tag="KPK87_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538795.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            9555..10241
                     /locus_tag="KPK87_002656"
     CDS             9555..10241
                     /locus_tag="KPK87_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG2538796.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(10368..10988)
                     /locus_tag="KPK87_002657"
     CDS             complement(10368..10988)
                     /locus_tag="KPK87_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538797.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(10978..12000)
                     /locus_tag="KPK87_002658"
     CDS             complement(10978..12000)
                     /locus_tag="KPK87_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBG2538798.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            complement(12001..12639)
                     /locus_tag="KPK87_002659"
     CDS             complement(12001..12639)
                     /locus_tag="KPK87_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG2538799.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(12757..13908)
                     /locus_tag="KPK87_002660"
     CDS             complement(12757..13908)
                     /locus_tag="KPK87_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2538800.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            14329..15141
                     /locus_tag="KPK87_002661"
     CDS             14329..15141
                     /locus_tag="KPK87_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG2538801.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(15230..15421)
                     /locus_tag="KPK87_002662"
     CDS             complement(15230..15421)
                     /locus_tag="KPK87_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538802.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT         5096 a         2605 c         1595 g         6162 t
ORIGIN      
        1 aatataaaat aaaacaccaa aattttgaat taaaatttgt aaaattgatt agaaaatagt
       61 attgcactat taccattaaa attctatata caataaaaga gaagaaaaaa ctgtacttta
      121 aaaataaatc ggaagaaaat aaattaatca tcttatatcc tcctattttt catgaactca
      181 agcataactt ttgaaaatat ttgttgacgt agtctagaaa caggtattct atctccactt
      241 tccataactg cattaccatt ttcatatcca tatacatatt gtaaattgat tatataacta
      301 cgatgacacc taaaaaaatt tgttggtaaa cttctctcta aattttcaat agtctcataa
      361 taatctacag tctctttctt taaatgaaca taaatctttc tttttataac ttcacaatat
      421 aaaatatcag caagttttaa tttccaatat gaataacctt tttgtattgt gatataatta
      481 ctttcattat catcaagttt tgtaactatt ttatccaaag ttttatatag tttctcatta
      541 gatagaggtt ttatcagata atttatagca tctacttcaa aagcatcaaa tacaaattca
      601 tttaaaactg taacgaaaat tataaagcac tcattaccat atgaccttaa ttttctagct
      661 gcctgcattc cgttaatctc ttccatctgt atatctaaaa atacaatatt aaacttttca
      721 tgattgcata gtaaatcttc tactttatta aaaagctcta tattatataa taaattcttc
      781 tgcttcatgt ataattctat attttccttt aaatgattta gcatatagga ttcatcatca
      841 caaatggcaa ttgaaatcaa tatatacacc ttctttctta tcattattct aattattata
      901 taaagttaat tttttctgtt caattaatat gatctaatat gttgaatatt tgttccaaaa
      961 tgtaataatc tcgcttttca tagtatatct aatttgaatt tgtgtatcaa tattataata
     1021 aactaattta tcacttttat aaatttaatt taaaaaaatg caaatttcga atttacctaa
     1081 taaaaaaaga tatcgtaaaa taattttaaa aaattatttt ttgatatctt tttttatttc
     1141 agtgttgtgt tgtattttta ttattcaaat actgtttgtt cttctatatc ttctgttagt
     1201 gcatttaaag cttttaaaac tatattcttt ctaatttttt tactttcctc ttctcctaac
     1261 ataggatttc caagtggata tggtatccct actgctggaa caattctatt agctcctatt
     1321 gtaagtgata ttggtactac tgtacatata tgaacaactg ctataccagc tctctcaatc
     1381 tcttttacca tcgttgctcc acaacgagta caagtacctc atgtagaagt taaaattaca
     1441 gcatctacat catcttctaa caatttctta gaaaactctt ctccaaatct cttagaattg
     1501 ccaacactcg ttcctgttcc tgtagtagtt ataaaataat ctactaattc tccaattaca
     1561 ccttcttttt caagctctct aactacatct agtggtacaa ctaaatttgg attttcaagg
     1621 acatatgctc tgtcataccc ccatgtatag ttgtaaattc tttatttgac atagaatcca
     1681 tatctgttaa atcgtaaatt ccatatttag tagcacttga agattcaatt ttgtctggat
     1741 tatcaattgg aactatccct ccagaagtta ctagggcaag ttttatctta gataaatctt
     1801 taacaggttt cataggtgta actctatcaa attcaggcat tggatactca gtttcaaagc
     1861 tttccccttt catcttttta accaacatat caattgctct ttcagaccct ctaagttcat
     1921 ggaagaaatt ttttcttatt cctctcatta tatatccttc ctcttcagga ggtcctattt
     1981 cctcacctct aaccaattta agacctagtt tcgtcattat tggaactacc tttctaagac
     2041 ttgctgctga atttcctgta gacactatat gcaaatctaa tttaaacata tctacacctg
     2101 gattttcttt atacatgcct gtaattacag gaatatttaa ctcttcttca actgccttac
     2161 aaatagtccc acaagctact ccatatctac ctgcattaaa tgctgggcct gcaataaata
     2221 aatcagggct ttcttctttt atcattttta aaagttcttg agttgcactc tcaatatttt
     2281 caccaaaata cgtatctccg caaattacag ttgagattat ctctgcatca tcattaaatt
     2341 gactctgaaa tgctattcca ggtcctacta caccagccct tttttctggc ggaatatctg
     2401 ctttttcctc tccacctata ccagcaaaga aattgtttat ataatgaacc actctgattt
     2461 tactcatctc ttaatcctcc aaatcacttt atagttatta catcagttag accaatgatt
     2521 acatcaacag aactttatat tcaaatactt atttaataat atcttgctgt caaatgatta
     2581 tagccatttg ctatagttga tgctattata atttgtagct ctgcatccat gcttccgtct
     2641 tcatttaagc atcctttata cccacctatc atcatttcaa cataatctaa agttcctata
     2701 attttttcca ttgctgggaa atgaaccact agatttcctt gaccacaaga aacaactgca
     2761 tctgcttcct tagtagcgtc tgctatagat tgactcttgc catcttttcc aggaaattca
     2821 tccgttatca aaactacatt tgctccaact tcactacatt ttctacaatt catcattagg
     2881 tcagtatctg gattcccata accttcttct gtcactagaa ctccatcaac tcctaaaaat
     2941 tctattaatt tagctaccat atcagagctt ctctctttat cgactaagaa tacgttctca
     3001 ttagtcaaaa taactcctat aaagtttaaa tcttttccat gtcttttata taagtcatgt
     3061 ataactggat tatttaaatg atggaatgtt gtaaccttat cacatggtgc tacacagttt
     3121 ccagatatta tagctccatc cattatttca gttggataca taaatgtagg cactatctgt
     3181 ttagcatcca ccccatagta ataagtgtca tgtaaaagtc cttgtgattg taacatgtga
     3241 acatatgcta ctgttggcag gtctggatat tcttttactt gttttaataa tggctttgtc
     3301 tcatatacaa tgacttcatc tggctcaact tctcttcctg cctcacctac atatgcacct
     3361 atttttaatc ctgcaattct agctgcttct tcataatcat gagtatctat accatccttt
     3421 gcttttataa caatacatat attatttgtt tttgaaaaag gtgtatattt agcagtagga
     3481 ccactcatat caattacgcc ttcttgaaat ccaactatat ttccacaagt tacaacacaa
     3541 gctcctaaaa gtacatgggt tcttccactt cctacagtct tcactttatt tataattcca
     3601 ggaaatattt tgctttcatc tccaatttta actctaggct ctattacatc tttaactgga
     3661 gctattctta ttgattcgcc aggtcttgca agttcaacat ttacacttac aatcctttca
     3721 tcttctaata ctatcttttc tatttcttcg ctactaatat atagtactcc atcttttact
     3781 tctgtctttt ctccaaattc aacatttttt ataaatatat tccctagctc tagcttcata
     3841 tgaaaatccc ccttaatata tttaatcgca attaaaagta tttattacgt ttattaatat
     3901 ataaagcatt ttctatgcca attatttaaa atatatattc tatgtattaa tgtcatagaa
     3961 attttcattt ttaatttaca ccaatattga atttagcaat aattattgta aatattaatt
     4021 tttaaaatta taataaaaaa agataaacag attttttctg tttatctttt aatacagttt
     4081 gagaaatttt tttctcactt aatgagaagt ttttttctca cttaatatta tactctgata
     4141 ttttattggt taactgtctt ctactaatct ccaatttttt agctgtattt gaaatattat
     4201 ttccacacaa agataaaact ttttcaatat actcacattc aaactctttt cttatttctc
     4261 ttaaagatct aatacttatt ttatcatcta tgtatacatt atttgatatt aaatttaaat
     4321 tgtcttttga taaattacct tcttcactta aaactacaag tctatttatt atatttttta
     4381 attctcttat atttcctgga taattgtaat ttaataaaaa atcctttact tccttatcaa
     4441 tcgagtgtat ctttttatca tgttcaattt ggtacttatt taagaaaaat tctataagag
     4501 tagttaagtc ttctcttctc tttcttagtg gtggtatagt tattgttatt gtacttattc
     4561 tataaaaaaa atcttctctt atatttccat ttgatatttc cacttttggt tctttattca
     4621 tagcacaaat taacctaaag tcaactttaa tagatttatt acttcctatc ctctctatag
     4681 atttgtcctc aagtgtcctc aaaagtttta cctgaacatt tagaggtata tctcctattt
     4741 catccaaaaa tagagttcct ttattcgaaa gttcaaatct accttttcta ctatctactg
     4801 ctcctgtaaa agaacctttt tcatgaccaa acagctcaga ctccaataga ttttctgaaa
     4861 aagaacaaca atttacaggt acaaatatct cattctttct tggacttatg ctatgtatat
     4921 atctagctaa tatatctttt cctacaccag attcccccaa gattaatata ttaacatctt
     4981 tacaagcagc tttttttgct atttttatga tattattaaa atctctattt ttagattcca
     5041 aagtaaattc aaaattattt ttttgtacag aaacagatat tttacttgcc tttacctttt
     5101 caacttcttc taataaattt tcaatgggat tactttttat aaaataagat aaagctcctc
     5161 ttttcatagc atcaactgca ttttcaatac ttccatatcc tgtaactaat ataacctcta
     5221 tatctctatt aatagattta attctatcta atagctgtac tccatccata tttttcatca
     5281 taacatcaga taaaaccaaa tcataatttt tatctttttt aattatattt atagcttctt
     5341 caccacttag tgctatatct acaagatagc ccttcttttg tagaattttt ttcattacca
     5401 ctccatattc tagctcatca tcaactaata gtattttcat tatatagtca cctctttatt
     5461 taatggaatt tttatatgaa aacaagttcc aacccccaat ttactttcaa cacttatatc
     5521 tccattattc tttttaatct cattataagt aatatataaa cctaaaccag ttccctcacc
     5581 aattggtttt gttgtataga agggattaaa tatgtccttt aggacatctt cttttattcc
     5641 ttcaccatta tctttaaaat ctataaataa acaatgattt ttttcataac agtatataat
     5701 aatttttcca tcttgatgta ttgcatctat tgaattagaa attaaattta taaatacatg
     5761 ttttaacgat tctccattta tatagcatat taaattatgt tcacagtcaa tttttatttc
     5821 aacattcttt aactgtaaca ttttatattg aagtttcact atattttcaa taaaattttt
     5881 tatatttata tgttctaaat tatcatctga tattcttgcg aaatttaaca agttggttat
     5941 tatattactt gctctatcta cattactttc tattgattta acacaatcat ttacttcttc
     6001 cattgtaacg ttatctttta agagatagca attatttctt attataccca aaggatttct
     6061 tatctcatgt gctaccccag aagctaattg tcctatagat atcattttgt tttctctaag
     6121 taatttcgcc tctactattt tatcattagt tatatcttta ataagaacta ctatatactc
     6181 agtattattt tttttatact ctacaggaaa agtgtttatc tttaaaattt tattgtgtat
     6241 ttctatttca ctagttttat tagtattttc agagaaggta ttttcaataa caccttttat
     6301 catgctaatc aaatctgcta tcttataact catttcaccc ataaagtcat atagtacttt
     6361 gttcgattct actatattgt tattttcatc tacaagcatt attatatctg taatactatt
     6421 aaaagttgcc tctaaagttt ttttattttc tattacctgt tctgttttcc ttttgatttc
     6481 tatctttaac aaatatgtat agctataaaa aacataaatt gatattaaaa ttatacctat
     6541 aaaaagataa atactaggca caattcctcg ctcatacaat atatcatcaa cttcattata
     6601 cgtagaatac cattttttct ttaaatcttt atacactcca tttttctgaa gtttaaaaat
     6661 gcctttattt aaaatactaa ccagctcttc atattgttta ggtacagcaa gaactgcttt
     6721 tttagtatat ataggattac ttaaaacact gtatttattt gaaagtccat aattatttat
     6781 atagtatcgc aatactggtt catcaccaac aactacgtct acttttccag acattaaatg
     6841 gttgactcca gtctttatat ctggagtcaa caaaatgttt atatcttgaa ttttttgctt
     6901 taaaaaattt atagaataat caccttctgg aattgctact gttttcccct ttaaatccat
     6961 gtaactattt atatcttggg actttttatc tttcaatata tttgcactca aagtgtatat
     7021 tgggtcagta aatataaatt tatttgccct ttcttttgat ggaatcaagt caaaaaactt
     7081 tatactatca tctattgaat ttacaaaaga ttctttccac caattatttg gtttaaaata
     7141 aaaatctcta ccaatttgaa ttgacagaga atttattaaa tcaacaataa ttcccttata
     7201 ctgtccatct tccttacctt tatatctcaa aggaggtgaa ctttggtcag agctgtaaat
     7261 caaattcttg tgtttttcta accacttttt ttcttcttct gtaaatggaa gtgacttctt
     7321 aatatattca aatacattta gattatactc catatttaca tacatatttg taagtgaaaa
     7381 tactactaaa aatgaataaa taatccctat aataacaatc ttttttttat tcactttaaa
     7441 actccaatct tcttttttac tataataaat tcaatattta ataacaaaat cctacttttt
     7501 tcattaaata tttatattta ttataaaact aattgaatag atgttgaaat tatagtcatt
     7561 actattccta tcaatgcttc atatggaatt aacttaaatc tttcacctat atccatgttt
     7621 atactacctg aactagtatg aaatagtgag ccatgtggta attgctcgaa tacactagac
     7681 ccagcattta ctattgatgc acctgaaatc ggtgatagcc caccatttat tatagcatca
     7741 tgaaaagtag atgatgctat agtagcacca gctgttgatg atgctgttgc taatgacata
     7801 agtatacccg aaactggtgc caataaaaat tgtggcatat ttaaaaattg taaagcacct
     7861 attgtactct gttgtaattc agacatctgt ataattcctg cgatagtccc tgttccaagt
     7921 aataatatgc ataccccttg catcttagat aatccaaaag ctaaatattc tcttgaattg
     7981 ttcaagtttc ctgtgacaat tagagttacg attcctccta ttggaagggc tatcatcggg
     8041 tctataacta taggagatac atttcctaaa aacaatagaa atatagctat aattggacca
     8101 cataatgatt taaataaact tggtaaatct tctctttgct ctaaaatttc atatgattgt
     8161 actttgtttc ctttatttat taatatactt gctaaaataa ctgtaataac aactccaata
     8221 attgctggaa ttatatttgc catcataaca ctagagagat tgacagaaaa attatctgct
     8281 actgctatag tatttggatt tggtgatatt atattacctg ctttaccccc tcccaacata
     8341 gctaatagta ttgacaattt agaataattt aattttctac ctatatataa tcctataggt
     8401 gctacagtta ttatagatac atccaaattt acacctactc cagcaagtac catagttgaa
     8461 atggcaatcg aaaataaagc cctcttttca ccaaatattt ttacaatctg ttctgatatt
     8521 ttatctactg cccctgtttt tattaaactt ccagctaata cacctgatgc caaagctcta
     8581 agaattgatg gtgatatatt catagctcca tttgccataa aatctacagt ttgtgttata
     8641 tttgctcctc caactaatcc tccaataaat gctcctaaca ttaaactata tacagcttga
     8701 aattttttta taattaaaat aattgctatt aataaaccaa atatagctcc aaaagtcgtt
     8761 atttgcataa tatcccctcc tatcataaat taactatctt ttgcttaatt ttatttaaat
     8821 atattttaag tattctgtat ccactttgtc aattatattt taatttattt aattatattg
     8881 aacatagttt tttcaaaacc taattctata tgttataata atttcatact acataatcat
     8941 tttacttgat tattttaagt taatattttt taaattaaaa agagaggata tttatatgaa
     9001 tgatttaaat ttaggggaaa aaatagctga agttcgaaaa aaacaaaact taagtattcg
     9061 agatttagca aaactagctg atgttacacc atcattattg agccaaatag aacgaggtct
     9121 tgctaatcca tctgtaaatt cattaaaatc tatagcctct tctctaaacg ttcctttatt
     9181 tacatttttt gtatctgaag tagataaaaa aaacttgata gtaagacatg ataatagaaa
     9241 aaaagttatt ttacctggaa gcaaagaagt tatatatgag attttaattc cagactctag
     9301 tggtaattta gaatttgcaa ttatggattt agctccaaac acttcttctt gtgttgatag
     9361 gattactcat aatggagatg aaattgcata tgttttggag ggagaagtta aactttttat
     9421 ggatgatgat gaatttactt tgtccaaagg agatagtgta aaagttcctt taggaacaaa
     9481 gcataaatgg caaaatgact caaatagtga aagtaaagta atttttgcag tcatcttgta
     9541 aaggagtaaa aaccatgaag acatataaat ttatattttt tgatgcagat gatactttat
     9601 ttgattttaa gaaaagtgaa tcacatgctt ttaaaaaact tctatctgag tttgatttag
     9661 aatttaattt tgagaattat atagagtcat atagaaatat aagtgataag ctttggctcg
     9721 accttgaaaa aaatattata actttgaatg aacttaaatt acttagattt gaactttttg
     9781 caaataaaat atctttggat gttgattctg aaactcttag caaaatgtac ttaaattttt
     9841 taggtgaatg tacattttta attccaggtg caatcgatat attacaatat cttaaaaaaa
     9901 agtatactat tgttataata acaaatggaa tttctgcagt tcaaaagaaa agacttgaaa
     9961 attcaaaaat aaaaggatat attgatggaa tggttgtatc tgaagagttg aaaatatcaa
    10021 aaccaaatcc tgaaatattt aaatacgctc tcaaaaagtt taattgtcat gataaatctt
    10081 ctgccttaat gattggggat agcttaacct ctgatgtctt aggtggaata aattcaggaa
    10141 tagatacttg ttggttaaat tccaataaca gtataaatta tactaatcat ataccaactt
    10201 atgaaataaa tacacttatt gaattaaaga aactattata gtttgtaagt ctgttcaaat
    10261 taaatagaat attactttgc aataattttt ttagtttata cataaaataa ccaaaaaaat
    10321 tattgcaatt attcatacac aaaacaaatc ttactcttga aaactatcta aaagcatttt
    10381 ctaggacatt tactacatct tgattctaaa ttttcttcat cccccattaa aataagcatt
    10441 gcaacacttt ttatggggga tagttgatga taatcattga tttctatttc tatactatta
    10501 tatttatttt ttacatagtt ataaatatcc ttttgtctct ctacttcaaa atctttatct
    10561 cctggatata tttcatgcga tattttgaga ctatcatact ttccttttaa atacattttt
    10621 attttctcat taatataatc taaacatact atacctattt tatccaatgc tagccctgcc
    10681 atcatattgc aattttccat atagtcattc atcttaaaat caattttatc ccctattgta
    10741 tatagtacag caaatgcttt tttacattct ttaggtgttt tattaatatc aacttcactt
    10801 atatatatct tgatatctaa aatatcattt atatttctta tttctctttc aacgatttta
    10861 ctaattgtat ctggagcttt tctatttcca taccccaaaa actttagcac aatattctta
    10921 tctataaaaa tatcatctaa atttatatcc tttaaaagat atttattttt attactatta
    10981 taattcatca atatcaatcc tatctaattc tttataacta attcttcctg cctttatagc
    11041 tgcttgtaaa ttttctactg gagtggtatt taagcagtta caaccaaaat cacataaaaa
    11101 gttatcatat cttctcatct tctttaacaa ttttagcgtc tctcttctaa tcttatctgg
    11161 cttagaacgt ccaagaagtt ttattgggtc taaattacca atcaaaacta tatctctagg
    11221 aatcatagga gcaatttctt catagtctaa aatttggtct aaacttactg catctatatt
    11281 acaacttagc atattatcta ataattctct ggtgtctcca catatatgca tacctttcca
    11341 acaatcaagt tcattgtaga ttttatttaa attttttact ataaattcat caaatctatc
    11401 tttacttagg gttactgatg ttggctctgc tattgaaata tattttgctc ctgctttatt
    11461 tgcagctaca gcatatcttc ttaccaattc agttgtaaat tccaataatt cttctacaaa
    11521 ttttttatca gttattatac atcttaaaag ttgagtagct ccagctaatt gacctgcaag
    11581 tgtaaatgga ccttgtatcg attcatatag tggtttttta atactttttg atataactga
    11641 taagctctct atattgagtg gcatacgtcc actcgtataa gggtctggaa catctaattt
    11701 tcttaattta tctctgtcag ttatagtatg tgtcaataca cttggaaaat cataatctgg
    11761 tcttaatgtc tctaaaccca gagtttcaca aaatgtaatc ccatcacaga atgagtatat
    11821 aaaatctgat tcaaaatact tgtccatagt ctttgctaat tctaattgca aatctacact
    11881 atcatatact tcatatgcct ttttatttat taagaacaat ccatttgtac ccatatcagg
    11941 cagaaaaaaa cctctatctt cactatttat atactgaagt aaagtcatat ctgaaaacat
    12001 ttaaacttca cctcctataa gtttatttat taatttaact gcttttggag catttgaact
    12061 gtatccatct gctcctattt cttctgcaaa ctccatactt acaggacctc caccaattat
    12121 tacttttggt cttttatcta tctcaatcat attaagttcg tctataaccg atttcatatt
    12181 ttccattgta gttgtcatca ttgaagataa gcctattata tctacattgt tttttatagc
    12241 ttcttctatt atatcttcac tttttctatt tacacctaag tcaataacct tatatccagt
    12301 ggcttctacc attactttta caatattttt tcctatctca tgagtatctc cttctactac
    12361 agacatcaga attactccct ttgatttttt ctttactttt ccagttccct tcaaaagatt
    12421 aataccttta tttaatgcat ctgtgcatac tataacttct gaaagatagt attccttatt
    12481 ctcaaactta tctaaagctc ctatcatacc tttgtttaaa ccatattcat aaatatcttc
    12541 cacatcaata tttttatcta tagcaatttt tactaaattc tctatattat ctacacccat
    12601 atttaatata cattctgcta tatcatcaag tatgtccata taatactcat cctttctaat
    12661 tacttacaaa attgcttttt caatatccaa taatttctct tatttaacaa ggactaactc
    12721 taggtatcaa tcaattttat ttaatatcat ctaaaactat tgataattta aatttgcagt
    12781 ccatggaaaa tctacaggaa atgcctttgt agcaaattca tatatatttt ttagatgttt
    12841 aaattcattg tactgttcct catttttttc aaaaaacagt ttattaagct tatccaaatt
    12901 ttctattaca agttcttctt ctttgtattt aataatatat ttattttcaa cttgtttaaa
    12961 ttccatgtat tttagtaatt caaaatctac atattgacta aaataacttc ttaaactatc
    13021 acacaactta gtaaaattta ttattttcat agttccttgt tgatagtcca aagattttgt
    13081 cccattacat ttcaactggt ctttaagatt tttcacatgt actttataat ttaaatattc
    13141 taatccaaat ttatttgcaa catatttcaa tatattttca acatttacag agtttaaccc
    13201 tatttcagca acttctccta cagtagaatc ttcttttttt attgttctta ttatcaaata
    13261 tcctattata ttattatttt cctttataaa taccttcttg taaccaattg gtccccaggc
    13321 aatagttgct gcatgcaata gtttttgaaa ctcatctctc gttcttataa atctagtact
    13381 attttgatta tatagttcaa tcattttatc taaatcatta tctttttcaa aattagtttc
    13441 atcaaattca actatttcat atgctatttt tacatcttca ggttttattg tatatttata
    13501 aaaactcttt accaaactac aatttcttct actgtataga cttcttgttc ctgatataat
    13561 tacaatatct acaccatcat aaaacatttt ttcttctact ttatccaata tcttagatga
    13621 atagcctctc ttttcataat ctggatgagt acatactcca cctatagctg caaccttcaa
    13681 tctgtttcct tgaatagata catcctgaat tagatagttt acatcagaaa ccactttatc
    13741 atcttctgat attataatca tattttctat attattttta cttagtaaaa gaggaaattc
    13801 ttccatcata gttggtttat gatttcttga tattctaaat acatagttta ttaattctat
    13861 tactttatca aattcttcta attttgcact tcttggtctt aattccattc atattcctcc
    13921 aaatattatt taagatactt aaaactctat tatctataat aaaacattta ttactacagt
    13981 tctaattata tcttttttct ttacaatttt taattgtttt tttagcaaat ttttataaaa
    14041 tataaaccta atttatagtt aaatttataa aataacccgt tatgtataat atattaaaat
    14101 attttctata tatttatatg actaagttct ataactaaat tgaaataatc actttttcaa
    14161 gaatgttata atttttttat tgtattcttt ttgctatagt ctatgaataa atcaatagaa
    14221 aatcttctaa gttgctttta aataatgact ttatggttca attgtactaa aatagtatat
    14281 gtaggttaca aataatctaa taaatattaa agtttggagg taatagatat ggcagattgt
    14341 aattcatgtc catcaaaggg aaattgtaat agtcaatcaa attgttctat tgaaaataac
    14401 cctaacaata aatttggaaa aataattggt gttatgagtg ggaagggtgg agttggaaaa
    14461 tcaactgtaa ctgctttact tgctaataag ctaaacaaaa tgggatataa ggttggtata
    14521 ttagactcag atataactgg accaagtatt cctagactta tgggtgtaaa aaatgtaaaa
    14581 gcttattctg atggctctta tatttaccct gtagaaaact caaataacat aaaagtaatg
    14641 tctataaacc ttatgataga tgatgaaaat gaacctgttg tttggagagg acctttactt
    14701 ggaggggttg taaagcaatt ttatactgat gtactttggg aagaattaga ctatttatta
    14761 atagatatgc ctcctggtac tggagatgtt gccttaacag ttatgcaatc tattcctata
    14821 agtggaatag ttatggtttc agtacctcaa gatttggtat ctatgattgt atcaaaagct
    14881 gttaatatgg ctaaaaaaat gaacataaat gtccttggag ttattgaaaa tatgagttat
    14941 atccaatgcc ctgattgttc taaaaaaatc aagttatttg aaggagaatc tactgaaaag
    15001 tttttagatg atttagactt agaactttta ggagaacttc ctatgactaa agaaattata
    15061 gatattactc ataatggagt tacagaaatt agtgatgatt tagattcaat attgactaat
    15121 gttgttgaaa aaattaaata aataaaaatt ttataatttt atatttatca aaaaaaaagg
    15181 cttgtattta ttttataatc atacaaataa atataaacct cttttatgtt tatctatgtt
    15241 ttaaatacac aaaatattct tctacttcat ctccattttt tacttttact aaatattcat
    15301 tataatgagc attatatggc tgtgtcattt tgcattctat tacattttct atgtcttcta
    15361 attctaaaag ttctacatcc tttaaaaatt ttctttctat gagttccatt aatctttcca
    15421 taatatatcc tcacttcccc tataattaag ataaacta
//
LOCUS       DAEMIK010000105        15321 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEMIK010000105 DAEMIK010000000
VERSION     DAEMIK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.105"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            677..1072
                     /locus_tag="KPK87_002663"
     CDS             677..1072
                     /locus_tag="KPK87_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538803.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNNTCTTE"
     gene            1115..3214
                     /locus_tag="KPK87_002664"
     CDS             1115..3214
                     /locus_tag="KPK87_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG2538804.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTIGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIILKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(3439..8649)
                     /locus_tag="KPK87_002665"
     CDS             complement(3439..8649)
                     /locus_tag="KPK87_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG2538805.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKRLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8664..11159)
                     /locus_tag="KPK87_002666"
     CDS             complement(8664..11159)
                     /locus_tag="KPK87_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538806.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEITKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(11796..12083)
                     /locus_tag="KPK87_002667"
     CDS             complement(11796..12083)
                     /locus_tag="KPK87_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538807.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(12103..12378)
                     /locus_tag="KPK87_002668"
     CDS             complement(12103..12378)
                     /locus_tag="KPK87_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538808.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(13014..13193)
                     /locus_tag="KPK87_002669"
     CDS             complement(13014..13193)
                     /locus_tag="KPK87_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538809.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(13478..14953)
                     /locus_tag="KPK87_002670"
     CDS             complement(13478..14953)
                     /locus_tag="KPK87_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2538810.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         5054 a         2687 c         1721 g         5859 t
ORIGIN      
        1 cttaaaataa tgttcattta taataatata tctaacacac tttaattgaa aaaaataaaa
       61 ctttgccttt cattcccata tataaataag tatacttttt gatgctctat tacaaatcaa
      121 ctaaattaat tgatataaat tatcattttc tgttgaaaat accattcgca catgctatac
      181 ttaattgcat aaatggtaat aaatgtcata tgattttaca aatctattaa taacaattat
      241 tacttttact gtattttaaa gtttttggac taaatatcaa gaactttcta aaatacaaag
      301 tacaaaaaat ttatataagt tataaattta tatgaactag ataagctcaa acaatatgtt
      361 atgtataaac tgtgtagtat tcggttataa atttatataa attatataag ctagataaag
      421 tattacctca actatataag ctagatatat aaattacata cataagctag atatataaac
      481 tgcatatata agctatataa gaattatttc aactatataa actacacatg taaaattcat
      541 gtaaatttac taatataaat caaataaaat tactaaactc tctaaactaa ttttgtaaaa
      601 catactctat ttttaacatt tgttacctta tacataagta caaacaaaat ctaactaaat
      661 gaaaggataa tgtaaaatga atattttaaa aatgaaaaaa acagggatat ttttaggagg
      721 agtacttttt ggtactgtag gtgttaagat actttcaagt gattgtgtta aaaaaacttg
      781 cactcgtgca acagcaggag cattgaaagt taaagattct gttcttgaaa caactacgaa
      841 aattcaagaa aatgtagaag atattttagc tgaagcaaaa gaaatcaacg agcaagaaga
      901 cttatctaca tctaaggtaa acgttgtaca agatactctt aaatgcgaac aagaagttct
      961 taaaactgaa actataaaaa ctgtagaaga tactaattct aatagcatta aatgtgaaga
     1021 aatcaaagat aacgtaattg aagaaaaaaa taatacttgt acaacagaat aaatttaatg
     1081 attattatga ttaataatat caggaggaag taaattgaat ttttatataa aacacgaaat
     1141 aagaggtcgt atacgctttg acttacaact tggcaaatta aacaacaagc agtccgatac
     1201 tctcttatat tatttaactt ctataaatgg tattaccaaa gcaaaagttt acgatagaac
     1261 tggtgatgca gtcatttatt acactggtga aagagaatac ataataagag aaatcactaa
     1321 attcagtttt aacaatagca aaatagaaaa cctagttcct gaacatacca gcagggaatt
     1381 aaacaattat tatagagaca aattagcaaa taaactgcta gtcaaaagta tctccaaaat
     1441 attttttcca aagtccttaa gatttttttt aattggactt cattctataa aatattttaa
     1501 aaaaggtctc tcttccctgc taaataaaaa aatagaagtt tctgttttag atgccactgc
     1561 tataggtatt tctattttta gaaaagatat caatacggct ggttcagtta tgtttcttct
     1621 tggtatcggt gaactattag aagaatggac tagaaaaaaa tctatagacg acttggctca
     1681 aagtatgtca ctaaacattg aaaaagtatg gttaagaaaa ggtgacactg aaatcttaat
     1741 tccaatttca gagataaagg aaggagattt agtaagtgta actataggaa atatgattcc
     1801 attggatgga gttatagtat caggagaagc aatggtcaat caggcatcac ttacaggtga
     1861 atcccttgcc gttaacaaaa aagaaggatc ttacatatat gctggaactg taatagagca
     1921 aggcaatatt gttatgtgcg tcaaggaaaa agctggtact actagattcc agaaaatagt
     1981 aactatgatt gaggaatctg aaaaactaaa atcttctgta gagagtaaat ttgaacatct
     2041 agcagataca ttagttccat acagcttttt aggaagtata ttaacctatg caattactag
     2101 aaaccctata aaatcactct ctatattaat ggtagatttc tcttgtgcat taaaactctc
     2161 tatccctatt tcagtactat cagccatgag agaatgtaac aataacaaca ttactgtaaa
     2221 aggtggaaaa tttcttgaag gtgttgcatg tgcagatact attgtatttg ataagacagg
     2281 cacattaaca aaagctcaac caactgtttc agatataatt acattccaaa attataataa
     2341 ggaggatatg ttaagacttg cagcttgctt agaagaacac ttcccacact ccatagcaaa
     2401 tgcagtagtt tacgaagctg aaaaacaagg gcttagtcac aaagaaatgc atacagaggt
     2461 agaatatatt attgctcatg gcatttcaac aaaagttgat gataaaaagg ttgtaatagg
     2521 tagcaaacat tttgtatttg aagatgagaa gtgtactatc ccagacggtg aggaggataa
     2581 gtacaacaat ctatcagatg agtattcaca cctatttatg gcaatatcag gtaaattatc
     2641 agcagttatt tgcataaatg accctttacg aaaagaagca aaatatgtaa tttcaaattt
     2701 aagagaatgc ggaataaaaa agattgtgat gatgactgga gatagcgaaa aaacagctaa
     2761 atctatagca agtaaagttg gtgttgatga atattattca gaagtcttac cagaagacaa
     2821 agccaatttt gttaaaagtg aaaaactaaa aggtcgcaaa gtaattatga ttggtgatgg
     2881 aatcaatgat tctcctgcta tttcagaatc agatgtcgga attgcaatga gtgaaggtgc
     2941 tgaaatagca agagagattt cagatattac catatcagca gataatttga ataatttaat
     3001 cattttaaag cagattagta ataagcttat gaaaagagtt gatttaagtt ctaagtttat
     3061 tattggcttt aatttaggtc tgattttact tggtgtcggt ggatttgtta gaccaagtac
     3121 atctgccttt ttacacaacg catctactgt cggaataagt ttaaacagta tgacaaattt
     3181 attggaaaac accaatactt ataactacca ttaatctcca ttacataaaa catatgatat
     3241 aaaaaattta taattcatct tttaaaaaga atatgtctta aaattagctt ttttctaatc
     3301 aaaattatat aaagcatact ttacaaattt taaactcaac ttatttctat tttttacaaa
     3361 aaaggagatg attcaaattt tcattttgaa acatcccctt ttgtaaattt ctttaaaatt
     3421 tatatattaa ttatattttc aaccaataat tgtattttca accaatcttt cttcatagca
     3481 ttttaaatca ttttgcttta gataattaaa ttcagactct acataaatac cacagcctac
     3541 caattcaatt gtcaaattag gtatattttc ctttagtata ctagtctgct cataaataat
     3601 tctttccaat aaatcaactt gtaatataga aaaatgcttc ataatctccc catcactatc
     3661 tatatctttc ttgatttcat accaactctt tgacaaaatt cccttcaaat caatataccc
     3721 tagactcctc aatatatcta tactatcgct ccatgcatac ttactaggtt tatcaaagta
     3781 tgattctagt ttcttcttct gctcatttgt aagtgaatca taagaaagct tcctattaaa
     3841 atgtgccttc aaagaaatct tagccataga atatggactt ctaccagagt ttaatcctgc
     3901 atttttgaac ttttcaattc ctttatcttc tactgaagac catattgagt ttattgaatc
     3961 tgaatcattg tatttaatat caagaccatg tgtaatattt atatagtttt gtggaaatac
     4021 atattgataa tagacatctt ttttatcatc atatagagag aagtatccac tatcatataa
     4081 atgaatatgc acctttactt ttaaactatc ctctaaaata tttcgtctaa ggttcgggat
     4141 atttgcatac atggttacat attttatggc atcaaagttt ttatttaatg gaacttcaaa
     4201 actaatttca tctcctgtca aaagcttttt cacttcttct tcattacaat catatagaga
     4261 atatgtattt tcaaatccca cacgtgattt cccatctaaa ctgcctgtta atgctacctt
     4321 caaaatatgg ttatctgttt tttgccattg gtaagcatct tggtcaagaa actctctaat
     4381 ctgtctattt tgctctttcg cctgatttat aacctcgttt tcatcttctg taatccctac
     4441 atggcatcta tttaagatat ttactatatt gcttccaaat gggaatgtcc atagttgaaa
     4501 agctgctgcc tcaacctgac acaaatatct aacctgattt aattcaattt gatggataag
     4561 ttttttctga tattcattca tctgattacc actatctgac atttgataaa tcaattctcc
     4621 cattgcaaga ttatcttttt tcttatcaat cttatcagta atgataattt gagtttccat
     4681 catatcctgt acttttttaa atattataga aatctcctct gaattttcca tttttaagct
     4741 tccaaacttt gtgcttaatt cagttaaaac ttccttcatc tttaatttac ttatctcatt
     4801 ttcaaaaata tcttgatttt taactgtact atcaaatttt tctacaaatg gtataacaga
     4861 ttctttcaaa gttttatctt taaattccac catttgagta ttcaattcat ctaatttctt
     4921 aatattattt tctttatctt taattgcctc tttaactacc tctattttac tctcaaaact
     4981 agattttaca ttaagacaat tattaacacc actaatcgca gtatttataa gagaacaagc
     5041 agaacttcca atactcaatg catttttaat tttagatgtc ttttgctgtc tttcaatagc
     5101 ttgtttactt ttctctagtg cagctaactt atctttttgc tgtttattat tgtaatttgc
     5161 ctccaacata cctatttctt ttttctggat ttttgtgtaa atttccttat actcactttc
     5221 acttaacccc tcttttgctc tttgaatatc gtcagctttg tctgcataat tatcatcatc
     5281 tatagactta actataatct ccttaatttg gtcaaattta gttcttcttt cttcatcaac
     5341 acctgccaaa ttataagtca ttttatctaa agcatttttt ctatcttcac ttagctgttc
     5401 tccaagtaca aatctcatac catcagaatc caatttaaaa tctgaaaaat caaccatcct
     5461 gttggtccat tggtattcct cttctttagc tgaaagattt tccttcaaag attttgcact
     5521 gtcaaatgcc cctagacaag cttgaccaac accactaaat gcagtcagta aactcaatgc
     5581 gctttgtatt ccagaaagaa ttaactgtgt tttcatttgt tcctcaagtt ttgcctgttt
     5641 cttttttagt tcctctatgt cttcttgttc atcttctttc atctttataa tttcttgaat
     5701 caacaggttc atttttgcat taacagaaag cattttattc tcaagttcat tttttaaaac
     5761 ctctatttgt tgatttccag attcaatttg agttaggata ctttccttat atgtattgaa
     5821 atatccttct tttaactcat ctttcttccc ttgttccaat tctcttaagg tcttatccag
     5881 ataatctgtc aaagaacgaa tatctgtaac acgatttcct ccaatttcac tttcttcaat
     5941 catctgcaca taaaatgctg tcttctgctc aagtgcaaat aaaatcatac tccattcgct
     6001 aaaattctga ttttttccta caaaagattc tattctatca gactgcatca atttatttat
     6061 aaggtattca tagatttcct ttgcttcttt ataaaattct ttctttgtat tcatatcatc
     6121 cagagaaagt actgctttat ttttcttctc aaaactatag tacttctgaa gtatttctct
     6181 atttaattgc atctctattt tctcaactgc atgaaaaatt tcataaatat gtgaaagttc
     6241 taaaatatca gcttctaaaa ttcctactgc acacaacagt ctaagatttc tataaccttt
     6301 aattccagaa aaaccaaatc tctcgaaaaa ttcatagaag aatgtatcta ttgacaaaga
     6361 atactcctgc tttaacaatt ccttttcttt tagtgcatct ccagagtgac cggcttttcc
     6421 ctctgctccg gctgaacctg aatcttcacg tttgctaatt gtccagtcct tatgttcttc
     6481 ataaagagca tttaatttct ctccatcatc tgctgcttgc ttctgattta aagcatgatt
     6541 aaacacatat ctatcaaaat aatagtagtt tctatcacta ttatccattg gaggagaggt
     6601 acctatatat cttattgttt cttcatatat acctctaatt ccaccaagac ctcctgcacc
     6661 accagctcct ccagagcctc cttttcctgc taaaccatga tttccatctt ttttacttat
     6721 agcacttttt aataaaggag cttttccaag gagataaacc tgtccagact tgcttccaag
     6781 acctgcattt ccaccattac ctcctgtgcc tccagttcca ccttgttttc ctttttctgc
     6841 ttttaccaca tagtattttg tacctttaac attagcaaat tcttttgact cttttttaga
     6901 acctatttta gcatttgccc cagcttctcc agttatccct gtatctcctt tttttccatc
     6961 ttgtccattt ccaccacgtc caccatctac gtaaagttta agttctcctc ttttttctat
     7021 ttctttaaaa gataatatga tatcacctga agtacctgca ttattgccat ttcttccatt
     7081 catcccgtcg taaatagaac tttctccaac caatttttct gcatccttac cagatacgtt
     7141 gattgaataa gtcccttctg gtatatgtaa tttttctgta agtagtacaa tattcttacc
     7201 tgagaagact tctttctcca tattacagtc aattttcaac tcacaatctc catacatata
     7261 tatagatgac aaaatattaa gttgataacc agatattatt ttagtcaaat ccgataaatt
     7321 cactttaggt ataggaatat atatagaaag tacattttct ttctcatatg attttatata
     7381 tgtacaactt tgaatcttct ctatcctata ctctgcatct tcaagccttt tggcttcttc
     7441 ttccctatat tcttcttctg ctatccaata tttgatatct ttcaatgtat cttctaaagt
     7501 attttttagc aatgatttta acaactcact tagaccaaca atatgagtct cactacagta
     7561 actatttaac tctaaaagaa gacgtgtttc ttctaatata ttcactttct gtacgccatc
     7621 accatatgaa agttcttgta caaactccat aagctctttc atattattag tcctactaca
     7681 taaatctgta tacatcttat ctaattgaat ctttttatct tttagactat ttatagaaga
     7741 tagaatggaa atagaatctt ccataccttt tttaacactt tctatataag gaactaaaag
     7801 tttttctagg tctttatagg ttacatcaac agctttttgt atttctattt tacaagaagc
     7861 agtaagcatc aatcccaaat ctgttttttc aaatgtttca ggtagatttt tcatttcatt
     7921 caacaacttt tctggctgtt tatctagtgg attacataat ttaatagata caccatctga
     7981 taatgctaca tttaataagt acactaattc cttacaaccg tcgtaatctt tctcttgact
     8041 atctaggtct tcttttgctt tttccaatat atttcttatt ttttcttctg ttacattacc
     8101 aattagtgtt ttaggtattt tattgataag aaagaagaaa ttttctttat actttgcata
     8161 atcaccaagc atatctttca aatttttgcc tgtttctatc aataatgtat gtctagagtc
     8221 cagtagggat tgatagtcta aaataactac catccctttt acctttgaag atgtggaaat
     8281 agtttccctt acaagcatag caccagcaat catatactct gctccacggt tgtcaaaaaa
     8341 acctgggcaa tcacataatg aaaaatctct atttgcaata tctacaactt ctggacataa
     8401 agtcttagaa tatacaccat ttccaatttc gacagctgaa tcttttgcag ttatatatat
     8461 ttgtccagtc tcttcatccg ttttttcttc catcacacag ccttttaaat aatttataca
     8521 agtactcttt ccaacgcctg aagcacctag gaataacaaa attgactttt cttttaagtc
     8581 atattttcta agctcgtctc ttccttcttc caaaaggttt acgatttcac tataaagttt
     8641 atttgccata catccatccc tttctatatt tcggaacaat agccttgata tttttcttta
     8701 ataagattct cttctacctt tgaaacagct gttgataact tagtatttgg agcattaaat
     8761 cctccattgt ctcctctatc tccatattca gcgtattctc ttgctggtcc tgttttacct
     8821 tcattccagc ttccaccact aaattctata tgatatttaa ttatactatc taacttataa
     8881 tcccaacttg taaaatatcc tgtatagtat acagtcttat ctataccact tggagaaaat
     8941 gaaagtgtat tatatccgtg atagccacca cttcctccat atccacctaa acctggtcta
     9001 ccatttggac ctgcttctcc atctttcact gttatatcac aatgattaat tattctattt
     9061 ttttgttcct ctgaatctga aatgaagtaa atattacctt tcttaccagc atttccacct
     9121 cttccagcat ttccaccagc tccacctttt tcaccttttt tgccacttgt acctttttca
     9181 aaataccctt taaacatctg tccgtggaaa taacttgtat atcctgcatc agctccatta
     9241 cttccagaag ctcctgtttc accattttct ccatctattc ctttagaacc atttcctcca
     9301 tttgcaatca gtgttagacg ataatttcta atttcttctt taatatataa gtaaatatta
     9361 cctgcatctc taccatcttc accatttttc ccatacatag aattatctac aagttctccg
     9421 ctctttccac ttacattaat ttttacatta ctacctacat caagattgtt agaagcaaga
     9481 ataatatttt tctctgatgc aaagttcatt ccaaggttat catccatata aatagtatct
     9541 ttagcaaaga acataaaagt agaaacattg tcatctactt ttctattttt gaaatattcc
     9601 aatacactgc taattactaa gtttggaatt aatgctctta tttcaatacc tttcttatca
     9661 ttaatattaa ttgtttttac tataaatgga tttaaaatgt atttaccaat aaattctcta
     9721 attatctcat taccttcttc tccatatgca cttaatcttg ttatactgtc ttctaactct
     9781 tcattttgtg taactgtaac atttgtcaaa attgaattaa aatcacttac actatataca
     9841 taatcctttt tatttgcaac ttccattaat ttttctctat ttggtaatgt aaatacctgt
     9901 ttttgcactt cataagaaac taaaactttt tctaaattct ttcttattat aatgaagttt
     9961 tgttccatta tcttcttact tgccttttct ataataaatt ccatatgtac agaaggtaat
    10021 aaaatttccc cagttccatg ttctgtatat ttacttaaag catcaattat atctaagttt
    10081 ccttttatct tattttgtaa tttcatgcaa ctatcttgta agaataagcc actttgtttg
    10141 gctaatacgt tgataaatct ctctatagta agacttgttg tactttccaa ccagttttta
    10201 aatactttta caacctcatc atattttgtt atttcttgaa tcattgttat acttccaata
    10261 atattgataa aatcttctgt atacgcctta acataatctt ctaggctaga tacgatattg
    10321 ttttgaattt cttttatcaa cgtatctaaa actaatcttg catctgcact aattggatat
    10381 tggaatgctg atgtcttatc tttaacttca tctaattcta gtacttgctt taataagcct
    10441 tccctctcat ctgatgcaag tggcttacaa agatgaatat tttttccatt atcttttagc
    10501 aattcttcaa ataaccaagc tttttcctct atattactgt ctaaattttc gcataattgt
    10561 tcatattttc tttttataac attatatatt tgtttttctg ttcttcctaa gaaacttttt
    10621 gaaatgaaaa aagttatact acccttaaaa ttttctttat gaagacacat atttaactgt
    10681 tggaacaggt ctagtacttt tgtaagtcta gaatctaata tatctgcttc tggtatcaat
    10741 acaattactg cacatataga tgcagaattt ttaaatactt cacctaatga aactgctgca
    10801 ctaatatcat gggctactcc cctattgtcg ctaaaacctg cagtatcaca taattttgca
    10861 tccattcctt ctattggttt tctcatattt acaatgttcg ggtataaagt ttgagaaaca
    10921 gctcctgaac caatctctgc cacagggtct aagcaatcaa tatatgatat accagtatct
    10981 tcatcttcta cttccatcat tacacatcct gcaagatagt taattgcagt acttttgcca
    11041 catcctgtcc caccaagaat tagaattaaa ttttgatttt cagccttatt tattgctact
    11101 tttccttcat ttaataagag tgttatttct tctaaaagtt gattgttatt ttctggcatt
    11161 tattttcccc cttggttata tattttattt aaataaagtt taatccctta agtagattgc
    11221 aaagttttta gactatattt aaatttgttt tttatttttt atttttaata aatttgcata
    11281 ttcactcaat ttgcattttc actcaataat tattcttctt tgtttattaa aataactgtg
    11341 ttgtataata taatctttac caacacagtt attttattta atgcgcttat tttattgtat
    11401 tttatgtaat acagttgttt tttatattat ctgtccattg ttttcctatg gttattacat
    11461 atagattttt aattatattt gtaccattaa tttaaacatt cctatactac tgcttaagtt
    11521 ttacacctat accagcttga atattttatt tttatttgct taaatagttc aactatactg
    11581 gtttaaatag gttatcttta tttgcttaaa tagttcaact atactggttt aaataggtta
    11641 tctttatttg cttaaattag ttcaactata ctggtttaaa taggttatct ttatttgctt
    11701 aaattagttc aactatactg gtttaaatag gtttccttta gtttaaataa ttcacctata
    11761 cctacttgaa tagttcactt ttatttgctt aaattctata ccaataccgg cttaaacatt
    11821 atactaccat gattgtattg tctatcttca actacaataa aaacaggttc taaatcttta
    11881 gtagatgtta tttttaattc taaaaaaaca ttatcagttg gtaatatttt tacatcataa
    11941 ttatttaaat tattattcgt gatactatta aatacatcac cttgacgttc aaaaaaaaca
    12001 ttatcaacta ttacaaaata aacatcttct tttctactat tttcaacctc tatagtattt
    12061 tgtttagtta catttagtct catcttccac ctcctattaa tattacttaa ttatattaat
    12121 acttgtaatt gttgcattat gttcatcaca aggaagaggt ttaaaaacaa ctatctctgc
    12181 taaataattc tgtggaggat ttgtaactga agtagcattg caaataaatt taaaatttgt
    12241 tcctgctaca acttgagtag aaacaataag tggttcataa ccaacccctt ctaacattcc
    12301 tacagcttca ttaaatactt ttaaatcttc ttcttcgaca gctctgaatt ttgaccatcc
    12361 accaacaaaa agactcatat aaaaacctcc tttatatata tatttattta tatatatagt
    12421 agtaccaatt ttctcaatat tcaatatttt atgtgtaact ggtcaatgaa aatgcataac
    12481 tggataaaaa cacttataaa ataacttaac tttacatata aatagcttac tttacatgta
    12541 ataagattat tctacaatat gaaatgcatc ttgttttgaa tatattctaa atacaataat
    12601 ggatattttc aattattata attagaaaat atccattact taacttgttt tatttaaaat
    12661 cacatagaga ctttattatt tttttattgc atgaatagct atttcaggta tcataaggtg
    12721 atataccccc tccttgaaac cacaaaaaac atgacaaact ttacgaatct tggcttaaaa
    12781 gaccacaggt acattcctta tttgtggatt ggagtagcca taacaagcaa attttacaat
    12841 ttttatgatg acaaaaatca ataaaatcgc ttagtatata acacgaaaca ctacttgtta
    12901 ctattaagaa tagttagata ggaagtatgc attataccaa taaaggaatc aataaagaaa
    12961 gttaaaacat ccctacaaat taaaaatctg ttacattttt ctatatttaa acgttattta
    13021 cttacaattg gagaaataac aggttttcct tctcggtcaa gtaaaggcgt aaatccggct
    13081 gcattgccat aatagtggaa tacatagttc acacctgttt ctttatccac ccaaatctcc
    13141 gttgcattta tttttccttg tgaatatact ttttcaaatc tatcttttcc cattttataa
    13201 tcccccatat ttttgattgg tctatacctt cttcacttaa gtcgaaaaaa tacataactt
    13261 atttttttaa ttctaatatg agcacgtaaa aatgtcaaac agcttattta atttcataac
    13321 ttcatgttac tgttcgtaac cttgtctgtt tacttacact gcattacagc accattttat
    13381 cctttacatt attgtattgc acacagtaaa aatgcttact ttatgggatt tttcaactaa
    13441 aaaaaggatt ttatctccac aaaacattta ctatttgtta caatatacct aattttaatc
    13501 tcatatcaac atttaatgaa gatataacct ttttcaaata ttgtaaagac tccttaaaaa
    13561 tattagaaaa agattctgca aatctctcaa catctccttt ttttaaatac ctaagtatct
    13621 caaaacatac atcaaaacgt gttttcaaaa tttcattata ttcttcatga aaaaatctct
    13681 ctaaatagat tccaaatatc atatattgtt ttaaatgcaa aaatgtttcc tgtaatacat
    13741 aaatttgtgt ataatctact atcttacaaa gtaaaacaaa atctggataa accttacact
    13801 cttcacaatt attttgtaag taccttaaat gagtttctat tttatctaca gctatcaaat
    13861 ccattttatt tcctagtaaa agtactacat cctcaagagt aatcgtaaca aactccatag
    13921 catccaaaag caaactcaac cttttagctc cttcttctgt atttatataa tcatcatcat
    13981 attcagaaaa aagtgttaca tatgttccct tgccattaat tgttttagca attcccaatt
    14041 catttagctc attataggca cttctaactg ttggaagaga tacattatat ctattaatta
    14101 tcgaagtaat tgatggtaga caatctccta ttttaatatt gccttcaaaa atttgacaaa
    14161 ttacatctga aacaattaag tcatataaat aacatttttc caacgttaca gaattaaaac
    14221 tacttgtatc tatactttgt ggagcacgat tttctaccat tttaagtatg gattctctac
    14281 aagctttata catatatgtt aatagttttt taaattcaat tagattcccg tccaatccat
    14341 ataagtaagt ttttcttacc tgctctatgt atttattacg aaaaaagtct atacaacaat
    14401 tatccaagtt atccaagtta tcgaaattat aggccaattg tgaaataatg acatcatgct
    14461 gaataatctc acaaaaatgg tgaatcagga aattatttgt tttacttatt agtttattta
    14521 tatattttag cctttgttct ttaaagacta aataattaga actatttttt tccatacaat
    14581 cgataatctc gtttaattct tcaaagtctt tagatgtaaa tcttttaata ttgtacatag
    14641 caatagaagg aaatattaaa ctaaaaatct tatatatttc tgatatttgt aaaatcttct
    14701 catataataa gtcagattct attgattgtt tactcgtact ctggattaaa actggtctag
    14761 aaccagcttt tgtctctata aagccctttt ctgagagcat ttttattaca gtttttatag
    14821 tattttttga taccatatat tcttctgaca tgctttttat agaatcaagc ctatctccaa
    14881 tagtaatttt accagtataa atttgttgaa ttatatgctc aaaaataatt aaatattgtg
    14941 gttttctctt catatttttc ctcttcgata aattagattt attgtttttt gtaaggtata
    15001 tttactttta tttaataggg caaaatagtt gcttattttt tcctcataat ctataattaa
    15061 tataaaccat ttatatatat ggtctaagtt ttataatatt gtaatgcagt aaaatagcat
    15121 attgtagcta tactacagca aattaacata ccattgtagt atattaattg gaataatttt
    15181 aagtacttca aaattccagc aaaacatata tgtgtttttg atattatgta tattcgttaa
    15241 tagaatagct atacttgttt tacataataa attatatgat acttgctata tgtaattact
    15301 atatttaata cacataacta t
//
LOCUS       DAEMIK010000106        15321 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEMIK010000106 DAEMIK010000000
VERSION     DAEMIK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.106"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            8..373
                     /locus_tag="KPK87_002671"
     CDS             8..373
                     /locus_tag="KPK87_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2538811.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            374..1813
                     /locus_tag="KPK87_002672"
                     /note="aph(2'')-Ia"
     CDS             374..1813
                     /locus_tag="KPK87_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBG2538812.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1943..3115)
                     /locus_tag="KPK87_002673"
     CDS             complement(1943..3115)
                     /locus_tag="KPK87_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBG2538813.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(3150..5225)
                     /locus_tag="KPK87_002674"
                     /pseudo
     CDS             complement(3150..5225)
                     /locus_tag="KPK87_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
     gene            complement(5240..5461)
                     /locus_tag="KPK87_002675"
     CDS             complement(5240..5461)
                     /locus_tag="KPK87_002675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538814.1"
                     /translation="MKERVDSHVHTLLRLRQTDRQTDRQTDRQTDRQTDRQVNSALFS
                     ILSIINRTHRLSSYKGEFVRFSMQRIYEK"
     gene            complement(5696..6331)
                     /locus_tag="KPK87_002676"
     CDS             complement(5696..6331)
                     /locus_tag="KPK87_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538815.1"
                     /translation="MEGCAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRTEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            6843..7985
                     /locus_tag="KPK87_002677"
     CDS             6843..7985
                     /locus_tag="KPK87_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2538816.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYVCDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSEKKRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSKKQEMLVEQMCTDIADVIESEGVFYE"
     gene            7978..8184
                     /locus_tag="KPK87_002678"
     CDS             7978..8184
                     /locus_tag="KPK87_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538817.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKGAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            8263..9456
                     /locus_tag="KPK87_002679"
     CDS             8263..9456
                     /locus_tag="KPK87_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2538818.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKEKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNLQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGQSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            9663..10991
                     /locus_tag="KPK87_002680"
     CDS             9663..10991
                     /locus_tag="KPK87_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538819.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(11119..12309)
                     /locus_tag="KPK87_002681"
     CDS             complement(11119..12309)
                     /locus_tag="KPK87_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2538820.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(12411..13790)
                     /locus_tag="KPK87_002682"
     CDS             complement(12411..13790)
                     /locus_tag="KPK87_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2538821.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(13821..15029)
                     /locus_tag="KPK87_002683"
     CDS             complement(13821..15029)
                     /locus_tag="KPK87_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538822.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         5060 a         2632 c         2448 g         5181 t
ORIGIN      
        1 tgataatatg gtttttgata ttgataattt aaaaggattt cttaatgata ccagttcatt
       61 tgggtttata gctaaagaaa ataataaaat tataggattt gcatattgct atacactttt
      121 aagacctgat ggaaaaacaa tgttttattt acactcaata ggaatgttac ctaactatca
      181 agacaaaggt tatggttcaa aattattatc ttttattaag gaatattcta aagagattgg
      241 ttgttctgaa atgtttttaa taactgataa aggtaatcct agagcttgcc atgtatatga
      301 aaaattaggt ggtaaaaatg attataaaga tgaaatagta tatgtatatg attatgaaaa
      361 aggtgataaa taaatgaata tagttgaaaa tgaaatatgt ataagaactt taatagatga
      421 tgattttcct ttgatgttaa aatggttaac tgatgaaaga gtattagaat tttatggtgg
      481 tagagataaa aaatatacat tagaatcatt aaaaaaacat tatacagagc cttgggaaga
      541 tgaagttttt agagtaatta ttgaatataa caatgttcct attggatatg gacaaatata
      601 taaaatgtat gatgagttat atactgatta tcattatcca aaaactgatg agatagtcta
      661 tggtatggat caatttatag gagagccaaa ttattggagt aaaggaattg gtacaagata
      721 tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag ttattttaga
      781 ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt ttagaattat
      841 tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt atttaatgga
      901 atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa ttgagcatta
      961 ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg atagtgtggc
     1021 atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata agaaaaaagg
     1081 ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa ctaatgtaaa
     1141 aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag gttataaaga
     1201 aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag aagaacaaaa
     1261 tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag attatacaga
     1321 tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata tattgttgcg
     1381 tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa gttttatgga
     1441 aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata atgattttag
     1501 ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg attttggaga
     1561 ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata gtgaagaaga
     1621 aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag atattgagaa
     1681 agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta ttgtttatgg
     1741 aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt ataaaaggac
     1801 ttataaagat tgattatata atatatgaaa agctattata aaagacatta gtattaaata
     1861 gtttaaaaaa atgaaaaata ataaaggaag tgagtcaagt ccagactcct gtgtaaaatg
     1921 ctatacaatg tttttaccat ttctacttat caaaattgat gtattttctt gaagaataaa
     1981 tccattcatc atgtaggtcc ataagaacgg ctccaattaa gcgattggct gatgtttgat
     2041 tggggaagat gcgaataatc ttttctcttc tgcgtacttc ttgattcagt cgttcaatta
     2101 gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga aaggcgtctt
     2161 cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttgatcg atataatcat
     2221 gaatcaatcg atttttagcc tcacgcgcta agttaatatc tgtgaactta aaaattcctt
     2281 taacagcttc tctgaaagat tttgaatttt ttttaggaat ggtggtaaag atatttctta
     2341 ggaagtgaac ttggcatctt tgccaactta cgttggtgaa ggattttcta atggcagaga
     2401 ctaatccttt gtgcgcatca gaaataacga gttccgtacc ttgtaaaccg cgttctttta
     2461 ggtattcaaa aaatgttgtc caggtctctt cgctttcgcc actttgaatc atgaagccga
     2521 taatttcacg gtcgccatct ttggttattc caatcgctat atgacagctt tttgagagta
     2581 ctcgattttc ttctcgtact tttatataga gtacatcggt cattaagtaa ggataatttt
     2641 tttctgataa taaacgattc tgccactcgt taaccatagg ttctagctgt tctgttaagc
     2701 tagaaacgaa ggacttagag acggatttac cacaaagttc ttccacaatt tttgatactt
     2761 tacgagttga aacgcctgat acatacattt ccaacattga agccatgagg gctttttcgt
     2821 ttcgttgata acgttcaaac actgtgggtg aaaaatggcc atcacgtgtt ctgggtactt
     2881 ttaattctag cgtgcctaca cgtgtcgtaa agctgcgctc ataatagcca tttcgttgac
     2941 tttgtcggtt ttctgttcgt tcatattctt ttgcttgaat atattctgtt cgttgatttt
     3001 ccattagttg attaaatacc gttgttaaaa tatttttaga aacgtcatcc tttacagaat
     3061 attcaataat gctttgaatc tcttcgcttt tcagtgtaaa atgtacttgg gtcatgtaaa
     3121 agtcctcctg ggtatgtttt tgtcgttaat aaaacagcta atacaccggt tgaaccagca
     3181 aacatagaag taaacaatcc tacgtttgtc atatcacctg tttttacagg gttcttttgt
     3241 ccgctaggct ttgttggagt agttggcttg ctcggtttct gtggctgttg tggacttaca
     3301 ggtttatttg ttactttttc ttttacggta actgtaattg ttttttcagt aattgcacca
     3361 tttttatcta ctacacggaa agttacatga taagtgccag ccttagaagt atctacatca
     3421 tttgcaataa tgttatcttt tgttaaaata atcgttccgt cctctttatc tgttgctgtc
     3481 acatttgcca atgggtcgaa cgtatcgcct acattcaatg taacatcatt tgctgaaatg
     3541 attggagcag agttaaggtc gcctgtattt tgtttcacag ttacagtaat cgttttttta
     3601 gcagaagctc cgtttttatc tgttacttta taagtcacat ggtaagtacc agccttagaa
     3661 gtatctacat catttgcaac aatattatct tttgtcaaag tgatagttcc gtcctcttta
     3721 tctgttgctg ttacatttgc caatgggtcg aaagtatcgc ctacattcaa tgtaacatca
     3781 tttgcagaaa tagttggtgc tgtattatct tctccttgtg agtttaacga taattctact
     3841 tttaaaacaa cgtttccgtc tttataagta ccgcaatcat actcataagt aactggtttt
     3901 tttacatcat ctaccgttat aattcctgta tctttatcta aagttccatt tgcagtaact
     3961 tttacttttg aaatatctat tccttgttca acatcttttt ttaagtcgat tttattccct
     4021 ttaatatcaa tataaccgtt gcctacaaaa gcatgattat tttcaagaac tttattctct
     4081 ccgacattta accagtataa atcatcgcaa attaattttt taatttttgt gtttttactt
     4141 atatcaagac ctcttatccc ttgttcaaaa atttcaccgc aacgtagcat ttcaagttct
     4201 aaattattgc ttaaatctaa ttttttaata gttgtatcat gacaaaaaag gcgtgtcagt
     4261 tttgtgtttt ttgttacatc taattcagct atatttgtca tataacatga tagttgtttc
     4321 aaattagggt tcttacttat atcaagtgtt tttacttttg tatctcctat atctaaagtg
     4381 actaattgcg gattatgtgt gacatcaagt tccattaatc ctgtattgct acaagatagt
     4441 tgttctaagt taggattgtt tgttacatct aaattggaaa tagaggtact atctgcaaat
     4501 aacgtattca atttttcatt cttagataca tcaagactgt taattgatgt attatcgcaa
     4561 acgaaagctt ccaaattcgg ataacgtgtt aaatcaacgc ttcttacttt tgaagaacga
     4621 caatctaatc tttttaaaga tgagttttta accggactta agtctgaaac agaagtcgta
     4681 gaaacatcta aagactgcaa atttgttaga tattgaatac cctctaatga agatatcgtt
     4741 ttcatatcat gcaagtttaa gaattgggct tttgatattt catcaacaga taacacacta
     4801 tcgccgtctt tatcaaattc tgcaatgatt tgttctctaa atactttgtc tggaaaatgt
     4861 gcttcatcaa tagcaatgct tccagctttt acttctgtat ttttgcttgc ttcaacggtc
     4921 ttatcttcgg taaccacttc tttttctggt gttgcaggtg tttcctgttt tgtactttct
     4981 tcttttaaag tttctgcatt ttcagcagtt tcttcctgtt ccttgtctgt tatattctct
     5041 tgtacttctg cgttttctgt tttctgttcc acaggaactt gctctgtctt gttttcttct
     5101 acaacggctt cctgcacaac ttctgtgttt tcctgtgcca tgacaggagt aggaagccct
     5161 gtaagtgcag ttgttgttcc aatcatcaat gcggaactta ctgcaaggac ttgttttttc
     5221 ttcatagttt gttcactcct tatttttcat aaattctttg catagaaaaa cgcacaaact
     5281 cccccttata actggataat ctgtgcgttc tgtttattat tgatagaatt gaaaaaaggg
     5341 cagaatttac ttgtctgtct gtctgtctgt ctgtctgtct gtctgtctgt ctgtctgtct
     5401 gtctgtctgt ctgtcgcaag cgtaacaatg tgtgaacatg actgtcaacc ctttctttca
     5461 aaaaaattca atccttgctt ctatacaaaa aacattatac tggaaacaaa cacctctttc
     5521 aatcttttta tgcgtgcaag ataaaaaaat tgagacatca cacttttcct tttttcctct
     5581 tgagtaccca caacagaaaa gaaaaagccc tgtcaagagc ttgccaccat tggtggtgct
     5641 ttgcactctt gatcgggctt tttgtttgct gtatcttctc gcaagaggaa aaagattact
     5701 cgtcctcatc atcaaaatgg ttattcctta atacttcccg taacagttcc atatcctctt
     5761 ttgagttcgg atttatcatt ttcacgactt gataaggtgt cctatattta tgtccctcta
     5821 tgctttcccc aaacatatcc atactgtata aatcatcata gcggtctagt aatttttgaa
     5881 acttcaaatg ctggataaat tctttgattg ggtaaaggtc ggacgcttca atgcttttcc
     5941 cgttgatata gtctgtaatg tattcccgta acttttccaa ctcatattcc agccattctt
     6001 cctcgctgtc aaagaagtta tcatagtgtc cgtgtttcag ttctgcattt agacgttctt
     6061 ctgttcttaa aaactgacag acttcttttt cagactgatt gacatacttc cattctcccg
     6121 ataataattc attcggcgtt acgtccagca cttccattat cttttccagc gtttcataag
     6181 caggatagtt caaccctctt tcaatcttgg aaaggctctg catattgata ccgattttgt
     6241 ccgcaagttc ctgttgtttc attcccctaa attttcttat ggtctgtata ttatttccta
     6301 aatggcttat tttcattgca caaccctcca tatctcgtta tatcttcact gttataagtc
     6361 tatcatgctt aatatgattt gtaaagacaa aaaaagatat tgacaaataa gcatataaga
     6421 attactatgc tattgtgagt gaataagcac aatagcatga aatacaaaaa cattcaaaaa
     6481 gaaataccca tctgattaaa cccgtagata tgaatattgg ttgaaatgga acttgttaga
     6541 aaaggcaaaa caggacttgc tgacaaagta tttctgacac ggtggcggaa gcctctcagg
     6601 cttaccgcca tgacaccttg tgtcaagggg aagaaatgct ttgtcggctg gcgagcctgc
     6661 gagccttgac tgtgcccccg ttatctaaca ggggggcact atttcacaaa agacaagtca
     6721 aaaaccatac aaccccaaac ctgtgagtag taatgagaag ttttaagaag atagtaattt
     6781 cacattcttt tgaatagtgg tttacaggta tttcaaggct gttgtgggat tggaggaata
     6841 caatgaacaa tttagaattt gcacttgaaa tgaagaacaa acgtcttgca agcggtcttt
     6901 cacaaggaga acttgcaagt cttacccatg tatcaagata tagtatcaac cgctttgaga
     6961 acggaaaagc caacgcaagt aaagaaacac agaacattat cctgcgttgt ctgaattact
     7021 atgtctgtga taagcctttt tatctgctcg ttgattatct gtctgtccgt tttccaacga
     7081 ctgacgcatt agaagttatc cgaaaggtgc ttggcatgaa agcggactat tttatccatt
     7141 atgactatgg gtattatggc tataaggagc attatgccta cggggaaatc aaggtcatgg
     7201 cttctgatga tgaacacatg ggtgtatttt tagaattaaa aggggcaggc tcacgcaaca
     7261 tggaatatgt cttacaggca cagaacaggg attggtattc atttttaaac cgctgtcttg
     7321 actgtggcgg tgttatccga cgttttgact tggcaatcaa tgatatgtgc ggtctgctgg
     7381 atattcccgt tttgtctgaa aaatacaaaa atggcggtgc ggattgtcgc tgtaaaaact
     7441 atgaaaatgt acaaggtgga aaactaagcg aaaaaaaacg taatttagca agtacccttt
     7501 atatcggctc aaaggcaagc acaaaatatt tctgcctgta tgaaaaacaa aaggagcagg
     7561 caaccaaaaa gaaacatacg gatattatca accgttttga aattcgtctg cgtgataaaa
     7621 aggcagtaca ggcagttgag gaattgttat tgacatataa ccctcatggg ttggtgtttt
     7681 accttattac tgattttgtg caatttcccg attatccgct gtgggagata ttcatttccc
     7741 atgacagttt accttttgaa atgaaccccg tacctgtcaa tatggaacgc actctgcaat
     7801 ggctggaaag acaggtcatg ccgtcaatcg tgatgataga ggaaatcgac aggctgacag
     7861 gctcaaacta catgaaaatg attgatgaat gtacccacct ttccaaaaaa caggaaatgc
     7921 ttgtagaaca gatgtgtacg gatatagcag atgtaatcga aagtgagggg gtgttttatg
     7981 agtaatgcac aggatattcc cgtatgggaa aaatataccc ttaccattga agaagcgtca
     8041 aagtatttcc gtatcggaga aaacaagtta agacgcttgg cagaggaaaa caagggcgct
     8101 ggctggctca ttatgaatgg caaccgcata cagattaaac gccgacagtt tgaacaggtt
     8161 attgacaaat tggacgcaat ctaatgcaaa tgagccttgt atgtgttatg atgaacacaa
     8221 gtcatatcaa ggctctttcc aacaaggaaa ggagcagaca ccatgaaaga aaaaagacgg
     8281 gatagtaaag gacgtatcct gcatactgga gagagccaac gaacagacgg gaaatactta
     8341 tataaatatg tagacgcatt tggaaacaca aaatatgtgt atgcttggag attgacaccc
     8401 acagacccga caccaaagga aaaacgggaa aaaccctcac ttcgtgaact ggaacagcag
     8461 ataagacggg atattgagga cggtatcgac agcacaggca agaaaatgac gctttgccaa
     8521 ctctatgcca aacagaactt acagagggca aatgtgaaga aaagcacaca aaaacaacgg
     8581 gaacaactca tgcggttatt gaaagaggac aaattaggtg ctaggagcat tgatacaatc
     8641 aagccctctg acgctaaaga atgggcgtta cgcatgaaag acaagggctt ttcctataac
     8701 accattaaca accataaacg ctcgttaaaa gcgtcattct atatcgccat acaagacgat
     8761 tgtgtaagga agaacccttt tgattttaag ttaagtgaag tcctagaaaa tgataccaaa
     8821 gagaaagtcg cattgacaga ggaacaggaa caagccttac tctcatttat caagacagac
     8881 aatgtgtatc acaagcatta tgatgatgtg ctgatactgt taaagactgg acttcgtatc
     8941 tcggaactgt gcggactgac agtagctgat attgatttca agaatgaagt tgtaattatc
     9001 gaccaccagt tactaaagag caaagaacag ggctattata ttgaaacgcc taagacgaaa
     9061 agcggaataa gacaagtgcc attaagtaga gaaacaatac aggcatttca acgggttatg
     9121 aagaaacgcc caaaggcaga accatttgtg atagacggac agagcaattt cttatttgtc
     9181 aatcataaag gcaagcccaa agttgcgatt gattacaacg ccttatttgt ccgtatggta
     9241 aagaaatata acaagcacca caaggacaat cccttgccac atatcacacc gcatacgcta
     9301 cgccatacat tctgcacaag actggcaagc aagaacatga acccaaaaga tttacagtat
     9361 atcatgggac attcaaacat tagtatcaca atgaactggt acgctcatgc gtccatagat
     9421 accgcaaaat cagaggttca gcgtctaatc gcatagaagt atttaccacg attttaacca
     9481 cgcttgatag cgaaaatata agaagataga cctagatatg tgaggtttac cacaaaagca
     9541 aaatgcccgt agagcctata aaataaggct ttgcggacat ttaaggagat ataaaaagat
     9601 agtcaaaaag acatatataa tttaatataa aataattata ataattgaaa aggaagtaat
     9661 ctatgatgaa aatagcagtc ataacaaccg aatttttaaa agagtttgta gataattcta
     9721 taaaaaagtt aaatattaat gcagaaattg aaatatatat ctatagagat ttttctcatg
     9781 ttggagattt atatttagaa atagaagata ggtttgatgg atttgcagtt agtggcccca
     9841 tccccaaaaa agctattaca aaaaaggctg gaactattaa aaaacctcta gttgattttg
     9901 gtactgactt acaaagttac tatgatatgt ttctaaagct tatctttaaa tataatacat
     9961 tagatttttc cagagcatac tttgacttaa cagactgggc agataatcca aaagatatat
    10021 catattattt atcaaatggc acatttggag acttaatgga tagtattgat gaaaatgcat
    10081 ctaaaatgag tttatctgac atagcaaaac ttgaaaaaaa gattgcaaaa aaacatatta
    10141 atctatggaa tgaagggaaa atcgattttt ctactactcg ttttagtagt attgttccta
    10201 tgttacaaaa tgcaggagta aactttcatt tcatataccc tgatatagat ttattaaaaa
    10261 atacttttaa taacttaata aaagatattc atttacatag aatgaaggga aatcaaccaa
    10321 gtgtaatata tataacagtt aaagatacaa attatgatga ctctactcta gaccaagttt
    10381 ataaaatatt aaatagtatt aaaaaagata aattaaccga ttttataatt gaaaaaaata
    10441 gtaattcttt taaaattttc actaaatgtg aaacgattaa atcattaact aaaaatttta
    10501 ctgcatgtta tataaaagaa tctattgaaa atagtacaaa tattaaagta tgtattggat
    10561 atggaattgg taatgatata aagcaagcta tgtctagtgc cataagtgct aataaggaat
    10621 ctaaaataaa tcctttaaat tacagtttct tagttaacga aaattatgag acaataggac
    10681 ctttactgtg tgataagtta cttactgttt caaataaagt aactccacat ataaatacaa
    10741 ttgccaaaag ttctaaattg tctactctta ctattcaaaa gattatatct gttactgaaa
    10801 ttctacaaag agatgaagtt actcctaagg aattatcttc atgtttaaat gtaacagtta
    10861 gaactgctaa tagatttcta agtagtctct taaagtctgg aaacgctact atattatatg
    10921 aaaaacaaaa ctcaacaaaa ggcagacctg aaagggtata caaattattt atatcagaaa
    10981 ataaatatta aaatccaaaa tgcataaaaa tattttatca tctaatatta agtaaaatta
    11041 tatatgactt tctaaaaggt atatctttgc atctttttaa gatatacctt ttatgatatt
    11101 taataatatt ttatgttatt atctactaaa attattttct aaccatttta ctgcacaatg
    11161 agcaaataaa gctgaaccat aaactaatcc ctcttcatta aaaattgctt taggattatg
    11221 cataccaaaa ttatatccct gactaatact tcccattcca atccataaaa atactcctgg
    11281 aacccttagt aaaacctctg aaaaatcttc tgaacccatt gcagatggaa agtctattac
    11341 attttcattt cctataactt ctttaacata agatacaatc tcatctccta catccaaatc
    11401 atttataact gcaggagtcc ccattttaaa ctccacttta gcatttcctc caaatgattt
    11461 agctattcct gtagatattt caataattct attttttaat ttttctctaa caaattggtc
    11521 aaatgttctt aaagttcctg acatagatgc tttatttggt atagcatttg gtacgtttcc
    11581 tgcattaaac ataccaatag ttaaaactac aggttctttc tgaggttttt ctcttgaaag
    11641 tatagtgttt aatgctattt gtatatgcga cattatgttt attgggtcca ctccaaattc
    11701 agggcttgct ccatgagttg cttttccttc tactgttatt tcaaatatat ccgcagatgc
    11761 catagctgga ccacgtgaat aagctacttg acctgtgtta agaaatttac ccaccatact
    11821 gtgaaatgcc atagctgcat caacctgagg attttctaat ataccatcat caatcattga
    11881 ttttgcacct ctggtagtct cttcatctgg ttcaaataca aactttactc tacctttaag
    11941 taagtgctta ttatccataa gtattttagc tgctcctaag agcattgctg tgtgattatc
    12001 gtgtccacaa ctatgagatg catctttatt cttactttta aaaggtaaat ccgtctcctc
    12061 cataatagga agtgcatcca tatctgccct taataataag gtctttccac cattttcact
    12121 gtctaaaaca gctactattc cattattaga cgtcaatttc ggagaatatc ccattttttc
    12181 taaagtactg gtaacaaagg ttactgtttt cggtagattg caaccaattt cgggattttc
    12241 atgtaaatac cttctccatc taataatttc cccttctatt gactgcgctt ctttaaaaaa
    12301 atttttcata ttccttcctc ctaatcagat agcaaaatct cactttcttt tagtaatgaa
    12361 atgatgctga tataaaaatt ataattttat atattaaatt tcatgtcttt ttaaggagtt
    12421 ggaatatcat ctggaatagg acatttatat gttttaccat tcatagattt atcaaattct
    12481 tcttttgcct ttttagctat ctctggatta tttaatattt tagctccaaa catagccata
    12541 atctttgatg catatatcat acctttttct cctatagaac ttcctgcaca agatgtaaac
    12601 tgccagctat gacctggtgc tcctagattc gtacaccctg taaaaaagta tgctgtcgga
    12661 actatatgca ttacatctcc aatatcagta gagccataac tattaaagtt tgtaacattt
    12721 cctccaccat aatatagatg agttccttct ggcaaattat atttacttat catagcctta
    12781 tattgttgtg ggctttgtct atctaattca gctgcaaaat ccaattcctc tcgagtccaa
    12841 ggctcttgtt taatctcatt catacattct gatacaagat ttgctaaaac atgattgttt
    12901 aaagtattat aacatcctcc taaaaactct acttcaactt cagtttcagt catcattgca
    12961 gctccttttg ctacctttac aagtctttca tatgtacttt ctacagcttc tctacttaaa
    13021 gctcttgtat aataccatac tgatgcttta tcaggaacta tgttgggtgc tactcctcca
    13081 tctgtaattg tataatgaat ccttacatca gaagtaacat gttctctcaa ataattagct
    13141 cccacattag taagttccac agcatctagt gcacttcttc cattttgagg gtctgaccct
    13201 gcatgtgcag tctttccctt aaagtgaaac tttacagagt taacagctgt tgaagtacca
    13261 attgtagctt cactaatttt atttggatga aaatttatag ctaagtctaa cccttcaaac
    13321 gctccacctc tagccataaa tcctttacct gttaattgtt cttctcctgg acatgcataa
    13381 aatactatag tcccacttaa gttattttct atcatctctc ttttaagccc tactactgcc
    13441 cctacatgtg ctgtacataa taaattatgc ccacatccat gaccatatgc acccaattca
    13501 aatgcttctt gctttgttga aactttttga tttaaaccag gtaatgcatc aaattctccc
    13561 ataaaaccta taactggttt acctgaacca aaactagctc taatagccgt ggctaaacct
    13621 cctacaccta cttccacatc aaatccttca cttcttagaa agtttgctgt attttcacat
    13681 gctttaaatt ctttaaatgc tttttctgga ttttcccata ttttcttact taaatcatca
    13741 agtttgaatc tttttttatc tatctcattt atagatatac ttcctatcat ttcctattcc
    13801 tcctataagt atatttacta tcaaccaaat ataattggac ctattatact agcaaatgcc
    13861 actgatgcaa tggttacagt aacaaatcct cctataatca tctttggtaa taaataatct
    13921 gttgcccttt gtttttcttc ttcagttcct tccatagcat caataccttc gttggaaatt
    13981 atctgtgttg caggataagc tagcatacaa gttacaccaa ttgcagctga cattcttgga
    14041 gaatatccca atagttttcc tacaattgca gtaaataata ttataaatac agaacctaca
    14101 acaagtattc ctatagttgg aacaatcatc ccaaacaatg ctgttggagt taccttagca
    14161 agtcctccag gtaacatcaa catacaacca aacattataa ttccattaga ttgagattta
    14221 tctaaaattc ctttttctaa aaaacctatt cgtgtaaaaa tcaaccctac taacaaacat
    14281 gctatattag gatttaaata atagtttgca ggggtaaatc ctggtattaa agtagccaat
    14341 cctacaaaat taccaatcat agcaacaaat gaaagctttg caagatatac taaagtagat
    14401 gattttttac ctaaatcttc tttaaaaatt ctcatacttg gaagtttaaa attacttttt
    14461 gactcatctt tcaaaaaatc cccgttatct cttttaaatt ttaattcttt atttatacaa
    14521 tatgtagtta tcggaatgcc aaagaacttt tgaaatgata atactagaat tacaaatgca
    14581 gcaagttcag gtctatttgc agcttctgca acagttgtta cgataatacc agcaacagta
    14641 gaacccgcta ttggaggtgc tgctattaaa gcatactctc taccaaataa catactaccc
    14701 actgttaaac agccaacccc tattcctaca atacctacta atgcaattac aaccgttttc
    14761 cattcatgac ataagtcttc aagatttata agtgttccta agttagttat taataaagct
    14821 ataccaaagt tgctcattgc agctaatatc cctgatgctc ctgtaatgtc ttttggtaaa
    14881 atatttgtcc aaaatcctag taaaaatata atacatacaa ataacaatgt tgaaattgca
    14941 ccttttgttt tcttagataa aaactcacta aaagcataaa tcattacaca tacagtaaag
    15001 caagtaagtg gattccaaaa attttccatt gttgtttcct cctaaatata ataatttttt
    15061 atcaataaaa caataattat aaaatgtata ttatgcagtt attcttaact acatatggta
    15121 tagcttgata ttttttgatg atattatata cgtcctaaat tattcctaaa atatctaaat
    15181 aaatataact agagttaact ctctaaatat taactatagt tatcaactta ctagatttct
    15241 ttttgatttt tactttctac ataattttac gagttagaat tgtacttcaa ctattctcta
    15301 tattagtata catgttggaa g
//
LOCUS       DAEMIK010000107        15096 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEMIK010000107 DAEMIK010000000
VERSION     DAEMIK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.107"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            237..2135
                     /locus_tag="KPK87_002684"
     CDS             237..2135
                     /locus_tag="KPK87_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG2538823.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(2263..3384)
                     /locus_tag="KPK87_002685"
     CDS             complement(2263..3384)
                     /locus_tag="KPK87_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG2538824.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            3583..3915
                     /locus_tag="KPK87_002686"
     CDS             3583..3915
                     /locus_tag="KPK87_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2538825.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            3902..4960
                     /locus_tag="KPK87_002687"
     CDS             3902..4960
                     /locus_tag="KPK87_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG2538826.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            4953..6023
                     /locus_tag="KPK87_002688"
     CDS             4953..6023
                     /locus_tag="KPK87_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG2538827.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHDEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            6125..6661
                     /locus_tag="KPK87_002689"
     CDS             6125..6661
                     /locus_tag="KPK87_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2538828.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            6777..7484
                     /locus_tag="KPK87_002690"
     CDS             6777..7484
                     /locus_tag="KPK87_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2538829.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGE"
     gene            7486..8202
                     /locus_tag="KPK87_002691"
     CDS             7486..8202
                     /locus_tag="KPK87_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2538830.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            8204..8962
                     /locus_tag="KPK87_002692"
     CDS             8204..8962
                     /locus_tag="KPK87_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2538831.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIACNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     SYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            9097..10485
                     /locus_tag="KPK87_002693"
     CDS             9097..10485
                     /locus_tag="KPK87_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG2538832.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLFLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(10561..11382)
                     /locus_tag="KPK87_002694"
     CDS             complement(10561..11382)
                     /locus_tag="KPK87_002694"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG2538833.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            11618..12787
                     /locus_tag="KPK87_002695"
     CDS             11618..12787
                     /locus_tag="KPK87_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG2538834.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            12970..13170
                     /locus_tag="KPK87_002696"
     CDS             12970..13170
                     /locus_tag="KPK87_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2538835.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            13591..14019
                     /locus_tag="KPK87_002697"
     CDS             13591..14019
                     /locus_tag="KPK87_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG2538836.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(14162..14917)
                     /locus_tag="KPK87_002698"
     CDS             complement(14162..14917)
                     /locus_tag="KPK87_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2538837.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
BASE COUNT         5917 a         1659 c         2461 g         5059 t
ORIGIN      
        1 aatactttta ttttagtata ccccatattt gtaaaaatgt taaactattg tatgtataat
       61 attttttcca ataaaaaata ttgcgaactt tttatacttg atatataata atataaataa
      121 aaacacgaat aaaacacgaa taatttgcaa gaaaaattca taaatgtaag gaaaatgaaa
      181 tttatcttgt atttaacaaa ataaaatata cataaaaatt atgggagagt ggaaaaatgg
      241 atagtctatt atacgtaata gagaaagata aacacactaa tgaagaatta agagaaatcc
      301 taaaaagcaa taaaaatata agatttgttt ccttaatggg ggttgattta ggaggaaatg
      361 caacagatga aaagattcct gtagaattat ttttggagga tatagataaa tttttggaat
      421 cagctataca gactgatggt tcaagtgttg agctttataa tatagctaca ttaaataatg
      481 cgaaagtaga tttaatgcca gataaaagtt gtcattggta tgtagattac aatatggaat
      541 atatagacga agaagtaggt ctacctgttg gaacattaaa gatacctgca tttttaatac
      601 atgataataa aaaagtatgt tctagaggag tacttcaaaa agcagataag tactttaaaa
      661 agtcaatgta tgaaatattt agagaatatc cacatgtaat aaataacata ggaatagatt
      721 cagtagatga tatagaggaa ataatgttaa cagcagctac agaacttgag ttctgggtta
      781 atactcctga ggataaagct gacttagaga aattatatgt atcacaaagc ctaaaagagc
      841 aatactggaa aagaactcat ggtattataa gaacttgttt agagaaaagt ttaattatac
      901 ttcaaaagct aggtgtaaac cctgaaatgg ctcacaaaga ggtcggagga atacaaagtt
      961 ctattagtat agatggaaga acaaaccatg ctatggagca attagaggtt tcttggaagt
     1021 tttctacacc attacaagca gcagacaatg aattattagt aagagatgtt atagaggatg
     1081 tatttacatc acatggacta gaagttacat ttaaagcaaa accaattcat ggagtagcag
     1141 gaagtggtgg acatacacat gtaggagtaa gtgcgaagtt aaaggatgga agtataaaaa
     1201 atctatttgc tccaaaagat ttaaaagaag attatctaag tgaactagga tatggggctc
     1261 ttatgggact tctttacaat tatgaagtat taaacccaat tgtaacagct tctaatgatg
     1321 gatttaatag attagttcct ggatttgaag ctcctgtatg tatagttaca tctttagggc
     1381 atagttatga gattccatca agaaataggt cagtgcttgt tggattaata agagatatga
     1441 aaaatccaaa gactgtaaga tttgaattaa ggtcaccaag ccctctatct aacacgtatt
     1501 tagtaatagc aggatgttat caaacaatgt tggatggtat aaaagcagca gctaagagtg
     1561 gtcttagtac aaaagaactt gaaaaagagt tatctaaaaa tgtaggtgaa gaaagtttct
     1621 atttagaaaa agatagagca tatagagatg aaaatgatgt atttgaacat tatagtttgg
     1681 aagaaagaaa tgcaagattt ggaataccac cagctacagt ttatgaaaat atgaaaaact
     1741 tagaaatata tgcttcaaaa ttaaaatctt taaaacaagg tgatgtattt actgatagta
     1801 taatagaatc atttaagatt ggagctatag ataagtggca aaagaaatta aaaactagaa
     1861 taatagaggc tgggattcaa aaaataagaa gtattgtcaa gatacacaca aaagaaaata
     1921 tggatgcgct tgatgaggta gtttggaact caatatcgga tttaaagttc aatattatga
     1981 aggatacttt aactagagaa tcactattca ctagagtgag agaagctata gaaaataagg
     2041 attaccagac tgcttcagat ttacaaatag aattaaagag aagtatggaa gaaatacaac
     2101 aactgtatat gcagtacaag aaaaatatat actaatataa caatttgatt ttgatatgat
     2161 aaaggcaatc aagtattatt aaacactatg agtaaattct caagtgtaaa atcatacttg
     2221 attgccttta attttatgtt tgaaattttt taatagtaca atttaactgt aatgattatc
     2281 tattaattct gatatataat cagcaatttt ctctggatga atgactgttt taaattcttt
     2341 taaatttttt ttcataaagt ttattataga atcaaattca tttgatacta atttataaaa
     2401 tatgttttta aggtctaggt catcactata aatcatgcca agacccttat ctattataaa
     2461 tttagcgttt tcaatttctt gtccaacttt tggcattttt acgagtactg gagtttgtga
     2521 ttgaatagct tcaaatatag ttgctcctcc aggcttagtt agcattaggt cacattctct
     2581 tattagatta tacatgtcgt ttacaaatcc aagtacttta atatttttta gaggtttttt
     2641 atgagttaga ttgtcatata attttttgtt tttaccagta actattgtaa tttctataga
     2701 attactatgt tcttcaataa attcatctat ccaatgcata aaatcctcat ctatatcaaa
     2761 tagacctctt cctccaccta gaagtaatag tctatattta tcacaacata gttctttact
     2821 ttcaatttga aatctcttat caacaggaac tccagtaacc tttatggagt caggatttat
     2881 tcctttctga aaaaatctat tttttatttc aggagaagga acaaaataca aatctgtatt
     2941 ttcaaaaacc cattccatac tatctacaac atctgttata actgtaagtg taggtatatt
     3001 tatatcagga ttttttatct taaagttatt tacacaagca gcagctagtg gaaatgtaga
     3061 gataatcaaa tctggattta tatcagcaat atactcagaa agtttaggtg tatataattt
     3121 atgagataaa tcatgtcttg aatcaaacga tttcttaaag taataatagt aattatataa
     3181 tccaggagta tattttgtat tgttttcata aagttttacc ataggcttat tcatcatggg
     3241 aatacttgca tttataaagt tttgtataac aacattatag ttactatatt tttcaataat
     3301 acattctttg actgcttttg cagcacttat atgtccagca ccaaattgag cagttaggat
     3361 taatattgtt ttatcatctt tcattttagc caatttatca ccactttcat aaaatatatc
     3421 tgattagagt attatacatt tatagaagga tttaatcaac aagaatattt aaaaataact
     3481 attgtgcaga acatagtact gtgttattat gaggatataa gaagttttaa catagtacct
     3541 tgtaaaacac aatactgtgt atttaaagaa aggatggagt atatgaacac acaatttaga
     3601 aagggtgttt tagaaatttg tgtacttgct ctcatatcta agaaagatat gtatggatat
     3661 gagatagtac ataacatctc taaagtaata gatgtaaatg aaggaacaat atatcctatt
     3721 ctaagaagac ttacaaaaga tttgtatttc gaaacatata tactagaatc taatgaaggt
     3781 cctgctagaa aatactatag aataacttca ctaggaaaag agaatttatc caatctgatg
     3841 gaagagtgga gagaatttgt aaaggctgtt gagattttaa taagtgaaga tgagggaggt
     3901 aatgaacttg aataaaactg agtttcttga aattttaaag gattatctaa aaaaagattt
     3961 ttctgagagc gaagttaatg atatattgag agattatgaa gagtattttg tagatggtct
     4021 tatagaggga aagagtgata tggagataat agcatcactt ggctcaccaa aatctatagc
     4081 aaaagaactt atcttacaaa ctaaagaaaa aaatattgaa gaggaaaaaa taataaatac
     4141 agatgttttg aatgataagt ttagtaaatt taaatcagat atgaaaaaag gttactctat
     4201 cttaaaagat gaagcaaaag aaaagtatag taaatcaaaa gaaaatatag atggaaaaat
     4261 aatgaataca gacatattaa atggtaaatt tagtagattt aaatcaaatg taaaaaagga
     4321 ttattctatc ttgaaagatg aagctaagga aaagtacaat aaatcaaaag aaaatataaa
     4381 tgataaactt acaccaagta tagaagaagg tgtaggagga ttatccagac aagttataaa
     4441 ggggttatta gtagtgttat ctttatttct aattgttcca gcatttgcgt ttgtatgtgc
     4501 tattgtatct gctactattg cattagtatg ctcattaatt gcttttgtta tattaattcc
     4561 atttgtgatt aaatttatag caatggttcc agatgttatg atgttttgta ttttcgcaac
     4621 catagcattt gtagggcttg agatacttgc atggcaaatt ttattatttg ccataaaact
     4681 aggtaaacaa ttactaaata attatataca ttggataaag agaaaaaata tttatataaa
     4741 agcaaatgaa aagatggaaa aaagaaaaaa agctacttta aatgatgaag atttgaataa
     4801 agacattaat gaagattcat ataataatga ttactacaat agagaagaag aaagcataaa
     4861 agaaactgat gaatataatg atgatagtat aaattttgag gcgatatata aggaccttga
     4921 agatatagaa aataagaaag gagaagataa acatgaataa aaaacttgct atatttgctg
     4981 cggttttatt ggtaataggt ataataggga caacatggtc agggatttta gttatgccaa
     5041 gcctaataaa ctttggttta gaaaaggaag ctgaatttaa aaaagaaaat aaactttatc
     5101 aagaaaaagt aaatattgac aagttagata tagctgttga caatataaac gttacaataa
     5161 agaaaagttc aagtgaagat gtgcgtgtaa caacaagagg aaataatgag ttttacaaat
     5221 acaatgtaac tttaaaagat aagactttag ttgtaaaagg tgaaagaaaa tatgaaaata
     5281 aaattaaaaa gattaaaaat ttcgaccaat tattaaatac atctataaat agtatgtttt
     5341 cacatgatta tagagaaatt ataatatatg taccaaataa tgtagacata aatgcatcaa
     5401 gtataagctc acacttattt gtatacgata atgtagcttc aaatactata acatataaaa
     5461 cttcttatgg aggttttagt agagctataa cagagaacaa agtaaataga ttagaaaacc
     5521 taaacttaat atctaataac aatcttcact tgtcaacaaa atcaatttta ggcgttaaaa
     5581 atgtaaacat agaatcagag tcattgtata tatcttcaga gaatgaagat gtatttatca
     5641 ataatataga agagtatata cctgaaaatg taaatataaa agagaaaata ggtagaaatt
     5701 caaattatga aagtgaattt tacttgtact ctgatatgcc aattgctaag ttcctggaca
     5761 tagaagttcc aaattctaag gtaagacttg atataccagt aaataagtat aaatttaatt
     5821 gtgatataaa gtcaaaagaa gcaattgaag aatttaataa tgatgaagaa tatgacaatg
     5881 attcatatga ctatgaacat gatgaagaac atagcaacaa gtatagaaat acgagggaaa
     5941 taaaagggct tttgaataaa aatctgtcga atttagagaa agaatatact ataaaaataa
     6001 attcaaatag cttagaattg taaataattt actataataa tttataaatt gatttaaaag
     6061 tataaaaata taatctatac tatggtataa taagtatcga aagagaatta ggagagggat
     6121 acttatgaat aaaatagcag taagtttttt aataattgct actactttat tgagtacggc
     6181 atgtatggat tatagcatca gtgcagttga gctagttgat tctaaagaat cagcagttgt
     6241 aaagaaggat gaagatgcaa aagaagaaac aactagtaaa atgattaatt ctaaaaaaac
     6301 tactaaaata cctatagaga taatttcaaa ggatgagaaa atagtaaagt acttacagat
     6361 tgatgaagaa agttcactta aggataaact gcgattaatc ttagatacat tatcaaatga
     6421 gtattttaat ggtttagaca tggaagtaga agttaaggaa aaagataatc ttgttaaaat
     6481 aaacttgata gaaccagata aaaaaagtag agtttcttgg aaagatgatt atttgaatga
     6541 acaaaatata atatatacta taaataatat tattaaaaat gtcatccaag aagaagataa
     6601 tagcatttgg attgaagaag tagagatata ctacaatgga aaattaatag aattaagata
     6661 aaaaaagatg tgtttttaac acatcttttt ttgtttttta attcttataa aatccttata
     6721 tttatgattt agaatgattc ttgtaaataa gtttgacaaa agaaaggatg aaaattatga
     6781 gtaattatat tttgcaggta aaaggagttt ctaaaaaatt taaggagcag atggcagttg
     6841 ataacatttc actagcaatt aaatctaatt ctatatatgg attattagga ccaaatggag
     6901 ctgggaaatc tactttatta aaaatgataa ctgggatttt aaatccaact tcaggaaaaa
     6961 taatgtttga gggacataga tggactagaa aggatttatc aaatattggt tctcttattg
     7021 agtctcctgc tatatatgag aatttaacag cacgagaaaa tttaaaagtt catactactt
     7081 tacttggtct tcctgactct cgcattgaag aagttttaga gactgtagat ttaagagata
     7141 cctttaaaaa gcgttcaggt caattctctc taggcatgaa gcaacgcctt ggtattgcta
     7201 ttgcattatt aaatcaccca aaacttttaa tcttagatga gcctacaaat ggtcttgacc
     7261 ctattggtat tgaagaactt agagaattga ttcgttcttt tccaaaacaa ggaattacag
     7321 ttattttatc aagccatatc ttgtctgaag ttgaacaagt tgtagatgaa ataggaataa
     7381 tttctaatgg agtaatgggt tatcaaggag aagtatcaaa agaacaaaac ttagaagaac
     7441 tttttatgaa agttgtagct gaaaatagaa agagagggga gtaatatgga aagatttatt
     7501 agagtggaat ttcaaaagat gaaaggaact tttggaatga agttgatatg gatagttcca
     7561 gtgttttgta tacttttgtg tggactaggt ggagggcaaa atgctgcttt taactggtgg
     7621 tatacgacat ttcttccagg agcattaaca ttaatatgtt ctttaatagt attaaaagat
     7681 gaaaaaagta aatatcgtgg tattttacca ttgccaattg aaatgaaaga tgtttggact
     7741 ggaaaaatga tttactgtat tatttgctgt tttatagcat caattatttc tgcaataggt
     7801 gtagtttttg ttgggataat ctataaagaa actatttcaa taggtcaaaa tatcattgca
     7861 actatattaa ttattttact atttatgtgg cagattcctc tttgtatgtt tttagcttca
     7921 aaatttggta tttttgttac agttctaata aatatgttct ttactattgt tggagttgct
     7981 gttttctcaa ctacagaaat gtggtatatg tttccatatt cgattccttc aagattaatg
     8041 gctgttgtat tgcatattct gccaaatggt cttcctgtac cagagggaag tgaattttta
     8101 aataatgctg ttattttgcc aggagtatta atatctattg ttttatttgt attatttgct
     8161 ttagctacaa gggtttggtt caataataga gaggtgaagt aatatgagac agttaataag
     8221 aatattttgt tctgatttaa taaaattaaa gagaaatttt ataatactaa tgcacatttg
     8281 cattgcttta atggggatgg gattgttttt atggtattat aaggtttcag gagctgataa
     8341 tatactaaag atagtagcat atttacaagt tatagcaata gcttttcctt tattgtcaag
     8401 tgttgtgtgt tcaatgtttg ttgaacaaga atattatgca gggaattata aacatatgtt
     8461 aacaacatct aatccaaaat atcttactct tataagtaaa tatataatct tagtatgtct
     8521 tggttttgta gcaacattag tgtctgtatt aggttttaaa tttggtatat ctaaggctta
     8581 ctttacatca agcttttata tgatttcaat tctaatttta attgcgtgta atttatttga
     8641 atacatatta catttattct taagtttaag gttcggaaaa ggaacatcaa ttggagtagg
     8701 tatagttgaa atgttacttt cagcattatt attaactgga ttgggttctg gtatttggca
     8761 gtattttcct tgtgcatggg gtgttagatt tataactatt tggtcgtctt tttcaagttc
     8821 taaaacggtt gaatatataa aggttgaatc aataaaatca tatcaatcaa caggtttaat
     8881 ttgtggtttt gtaacaattt tagcatttgt aattttatgt atttggtttt ctaaatggga
     8941 aggtaaaaaa tcagaagaat agagtaaaaa ttgactgttt atttatatta ttatgttaaa
     9001 atataactta gcaataaaaa gatataaaat aatcaaataa aaaactggtt gggtattaat
     9061 tatgaattaa ttaaactatt ggaggacagg gtacaaatgc ttggaggtaa aaaatctaac
     9121 aacaaaaaag ttgaaaagtt aagaactatt tttataaaat atttatcttt attctttatt
     9181 atgacaattt caattgtact ctttttaatg ttatcatttt ctgtattatt atcatcaggt
     9241 gtaattttac cagctaatta tgcagaaaaa caatttaata aatataaaga acagattatt
     9301 tcaagtgaaa aagtaactga agatataatt ccaagtatat atgagtatgg ggtttatact
     9361 ttagatggga atttaatttc aggtaccttt aataaaaaag aatcaaaaga agtatggaat
     9421 ttaatgagag atattgaaga aaggcatgca tattcagaaa gttatataaa gttttttaag
     9481 aaagatgaag tttttataat aaaatacaaa atagtatcag agtatagttc tccaatttta
     9541 agagcatatt taccaaaacc agagacattg ggtatgatta tttttagtat aatatttttt
     9601 attgaaattg ttatattatc aaaagtattt ggcaagaaat ttaatattga aatggagctt
     9661 ttaaaaaata ctacagaaaa aatagaacaa caggatttag attttgttgt agaatctagt
     9721 aaaatttatg aaattaataa tgtccttttt tctatggata aaatgaagtt ggcgttaaaa
     9781 gattctttag aaaaacaatg gatgctagaa gaaaatagaa aggaacagat atctgcactg
     9841 gcacatgata taaaaacacc actaacaatt atacatggaa atactgactt attaattgaa
     9901 ataaatgaaa atcctgagtt attagaatac atggaatata tctcaaaggg tgtcactcaa
     9961 atagaaaaat atattaatac tttaatagat atatcaaaaa cagagaatgg ctatatatta
    10021 aataaagaag caatcagtat gagtgagttt ataaaagatg tcttagtgca aatagaagca
    10081 ttagctagta ctaaaaactt agatgtggag ttttataaac aagataattt gcctaaaaat
    10141 ataactatag atagagaact gttattcaga gctattatga atataatttc aaatgctata
    10201 gattattcac caagccagag taaactatac ataagtgtat cagtatgtaa taaatactta
    10261 aaatttgtag ttacagattg tggaagtggt ttttctaaag cagatttgtc aaaagctaca
    10321 gaacagtttt atacaggaga tttgagtaga aattctaaat ctcattatgg aatgggactc
    10381 tatattgtaa ataatattgt acaaaaacat aatgggattt tatatattga aaattctgtg
    10441 aaaactggtg gtgctatggt aacaattgaa ataccaatag tttagtaaat aatatccatt
    10501 ttgtaaatac ctaaatttta tgatttatag gtatttataa aatggatagt ttttttgact
    10561 ttatatatta gttaaatctg ataaaaagtt ttcaaacata actccatcat ttctatcagt
    10621 atcaaattga gatgtgtaat ttatagtttt atatataaaa tctgttgcag tattaaccgc
    10681 tactcccaaa tcatgtttat ttactaacat accacaaagt atagatgtga atatatctcc
    10741 agttccactg taagaacaat tattatattt gactgatgta aagaatacat ggtcattatc
    10801 tctatcgtaa gccaaattta atatattatc atcttctaat ataccagtaa tcacgacttt
    10861 gctaggtccc aaatcagata cagattttgc aatatatata agctcatctc tattataatc
    10921 gcttttagta taatcatttc cagtcaagaa acaagcctct gttaagttag gagtagttaa
    10981 atctgaatgt ttaactaatt ctttaatttc ctgtctcatt tcttctgtga atattggata
    11041 cataactcca ttatcaccca tcacagggtc aactacgata aaagcattgg gataatcgtt
    11101 tataaaatta gccactatct caatttgatg ctttgaaccc aaaaaaccac tatatatact
    11161 atcaaaattg acttttaaat ttttccaaac atttgaatat tttaccattt catcagtaaa
    11221 gtctaagaaa gtaaattcag gataaccagt ttgacttgaa agtatggccg ttggaaatgg
    11281 acaacattga acctttaatg ctgaaagtat aggtatagca accgataatg agcattttcc
    11341 tattccagat aagtcattaa ttgctgcgac ttttttttgc ataaaaaacc ctccattagt
    11401 atgtattaat tataatgata ccaaaatatt tatatacata tatattacta taaaaaaaag
    11461 aaaaaagtag attataaaat aaaaaattgt aaaaaaaaca gtgtattgat acaattaatt
    11521 tttaatattg gacataataa aatagagaat tattaaatat gaaaattact atatgtgaca
    11581 gaatattctt tgtaatttga tttgtaaggt gtactatttg ggaggtgtag ctttgaaaat
    11641 actgaaaaat aagaataagt acatcaaagg tataatggca tttgtatttt tgtctttagc
    11701 tactacagca ggtgtatttt taatcaatga tttaagtatg aaaaatatgg aagaaaaagt
    11761 catggaatct gcaaatgttg aaggtgatga tgtaaaagac caatataaac tattgcttga
    11821 aaacttattt gactacagaa ataaggcaat attagagaaa aatgaaaaca ttttaaaagg
    11881 tctttatgag acggataaaa aatttggtct gtgggcttat gaaaatgaag taaaaaagat
    11941 gaagtattta gaaaactggt ctagtaagca aggtgtaaaa tttaaagaca taaagacaag
    12001 ggttaagatt aaaaaaataa aagaaaaaga aaaagacctc tatggtataa tctgttcagt
    12061 atcgactgaa tataagtatt catatgaaaa tcaaaaggac aaagaaaatg cctttagaat
    12121 aggtacatat cattatctaa atgtaaaaat tatagataat caatatgtca taactaaaga
    12181 gtggtataca gacccttttg cagactcttt aaatttagaa aatataaaat ctgatgatat
    12241 aagaaactat attggtcaac aagatggtgt agagttacag cttacagatg aacaaaaaaa
    12301 ggctatagat tatgcacata aatactgtgg agccgcagct gatgaagaac atggtatgaa
    12361 atttaatcca aactataggg attataatcc agaaggtgga gattgtgcaa actttgcatc
    12421 acagataatg tttgaaagtg gaaggttcaa aaagaactca atttggaatt atagcgagag
    12481 agcaggtaca aaagcatggg taaatgcaca agcttttaaa aattatgcac tgtatagtgg
    12541 aagaggttca ttaattgcta aaggttccta tgaagaagta tacaaagaag cttataattt
    12601 acgtccaggg gattttgttg gttatgagaa gaaaggcaga ataacacatg tttcgacagt
    12661 gacaggatta gactcaagag ggtatccttt ggtaacttgc cataatactg atagaatgtt
    12721 ggtgccatgg gatttaggct ggagtgataa gtccataaga ttccatataa taaaaataaa
    12781 ttattaaaaa attataaaga aaaataaagt gtaaaagata ataatcattt aaaattataa
    12841 agtaaaaaaa ttgattatta atgtataaaa ataataataa ttgacaaaca tagctatata
    12901 tgatagagta gtaataaggt tacagaaaag agtagctaaa tatttgtgtt tttttaggag
    12961 gatttattaa tgaaaactgg tgtagttaaa tggtttaaca acgaaaaagg atttggattc
    13021 atatctgtag aaggtgaagg agatgttttc gtacatttct cagctataac tggagatgga
    13081 tacaaaacat tagaagaagg acaaagcgtt gagttcgaag tagtagatgg agctaaaggt
    13141 cctcaagctg ctaacgtagt tagattataa ttaaaaattc atatatatta gtgttctagt
    13201 aatatgagag ataagccgct aatcatttag cggttttttc agtttttgta aataaaatta
    13261 atagcaaaat aaattaaaat tattaaaata aaatcattaa taatgagata aatatgttaa
    13321 aattaatagt gtagagtaaa attattatgg ctaagtttaa gtcaattaat ttttagtaaa
    13381 ttttctaaaa agagaataag tgatactgat agaccatata aaaatattaa taaagcataa
    13441 taagattaaa atgagcaaag gaaaaacgct tgttacagcg tatagtatta agaatactta
    13501 gaaaataaga aaggagaaag ttactgcaaa aaagtaacat tataaatatt gagtaatatt
    13561 tataagtttt cagtaacggt gggtagtgta atgaacatat tattctttct aacaccaaaa
    13621 agtgaagtag catatatata tgaagactac actataaggc aagccctaga aaaaatggaa
    13681 tatcacaaat actctgcaat tccaataata agtaaggatg gtaaatatgt tggaactata
    13741 acagaaggtg attttttatg gacattaaaa aatgatttaa atctggactt aaagggattg
    13801 gaagatgttc cagtgacaga tataaataga aaaatggata attctcctgt ttccatcaac
    13861 gcagatattg aagatttagt tataaaatct ttaaatcaaa atttcattcc tgttatagat
    13921 gaccaagaca catttattgg gataataaaa agaagagatg taataggtta ctgttatgag
    13981 ataatacgtg gatataaaaa tttagctaat gataattaaa aatcttattt aaacttttaa
    14041 gctgagttct ttacaataaa agaatgataa tatgaaactg aatatttaaa ctttaatata
    14101 aaaagctgtc ttttctaata aatattgtga aaagacagct tttttatata tgaccttatg
    14161 attaatcgtt gtattttaca aggtttccgt acttagcatt aagagcattt atcttatttc
    14221 cataaacttc attttgtttt tgatataata aacttttttc aacttcagcc ttaacttcat
    14281 caaatgtaga ctctgtactt tcttgaaggt cttctaattt tattatatgg tatccaaatt
    14341 gagttttaac tggttcactt acttcacctt tagccataga gaacacagct tcttcaaatt
    14401 caggaaccat ttgtcctcta ccgaatgttc ctaaatcacc acccatatct ttagatgggc
    14461 aagaagaatg tttaagagct gcatcttcaa aagatatttc ttctgatttt atttgagcaa
    14521 gtatttcttt agctttttca tcactatcta ctaatatatg tttagcagtt gcactttctg
    14581 gtttagagaa agagctctta ttagcttcaa agaattttac tttttcatct tctgttacat
    14641 taacactaga taaaacttta tttatagcaa attgctttaa tacattttct tctatctttt
    14701 tcatttctaa tttaaatatt tcttcttcat ctaatttttc ttctttagca tctaagaaga
    14761 ataattcttg attcactaaa tcatttaata agtgtttttt tccttcttca gttttaaatt
    14821 gcatagcttg ataagggtct aatgatttta atgcattttc aatatcaata ttagttatct
    14881 ctttttctcc aactgttgct aaaacttttt tttccataat agttaactcc ttcatagtgt
    14941 atttcattat taatataaga ctaccatctt aaataatatc agaaaataat gtagatgtct
    15001 aatatttaaa gccatattaa gtcaaataga attaaattta gtagaacaaa gttattacaa
    15061 ttaataaaag tgagctcata atacccacta cacctt
//
LOCUS       DAEMIK010000108        15096 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEMIK010000108 DAEMIK010000000
VERSION     DAEMIK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.108"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            471..2705
                     /locus_tag="KPK87_002699"
     CDS             471..2705
                     /locus_tag="KPK87_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2538838.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNIGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLAMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2736..3581
                     /locus_tag="KPK87_002700"
     CDS             2736..3581
                     /locus_tag="KPK87_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2538839.1"
                     /translation="MFKEIFLKTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFMDSHNIQLDWEKNYPYDKISNWIFEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3821..5554
                     /locus_tag="KPK87_002701"
     CDS             3821..5554
                     /locus_tag="KPK87_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2538840.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5946..6174
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6011..6039
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6453..6605)
                     /locus_tag="KPK87_002702"
     CDS             complement(6453..6605)
                     /locus_tag="KPK87_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538841.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
     gene            7729..9084
                     /locus_tag="KPK87_002703"
     CDS             7729..9084
                     /locus_tag="KPK87_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2538842.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(9202..9870)
                     /locus_tag="KPK87_002704"
     CDS             complement(9202..9870)
                     /locus_tag="KPK87_002704"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG2538843.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(9937..10365)
                     /locus_tag="KPK87_002705"
     CDS             complement(9937..10365)
                     /locus_tag="KPK87_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2538844.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            10472..11065
                     /locus_tag="KPK87_002706"
     CDS             10472..11065
                     /locus_tag="KPK87_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG2538845.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            11185..12120
                     /locus_tag="KPK87_002707"
     CDS             11185..12120
                     /locus_tag="KPK87_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG2538846.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(12391..13335)
                     /locus_tag="KPK87_002708"
     CDS             complement(12391..13335)
                     /locus_tag="KPK87_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2538847.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIMYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            13653..15083
                     /locus_tag="KPK87_002709"
     CDS             13653..15083
                     /locus_tag="KPK87_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2538848.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         5780 a         1983 c         1990 g         5343 t
ORIGIN      
        1 atttattata gtctttaaca aaaaatacaa gaataaactt aattttaata actaaaatta
       61 attatgcaaa attattaata tggtattaaa ttatgtataa aaagttaatt atgaataaaa
      121 taattatgcc tttgaaaaat cattaaactt aaaattaatt ataaaaaaac tctaccttac
      181 taactataca gctcacaatc tatctattta tgaaaaaata tgatataatt gcattcaaaa
      241 aggaaatctt tcaatatact agaatttaag cattaactaa ttttatctaa tattattact
      301 aaactaaaaa atatttgtat ttgtagcatt ggagcaaata acttaatgta tattcatttt
      361 aaatacttat ttaaaattta gcatttaaat tatttacaaa ttaacattta aatacttcta
      421 gcataaatac agttgtcatc aaaatttaaa gtatcttatg gaggaatacc atgcaaaatc
      481 ataattatat aaaaatgtta aataaaaagg tagaagtatt atctaaggaa ttagatagta
      541 gaaattttaa attaaaaacc ttaaatgaaa tagatttttt ttctaaaata ccaaacagag
      601 aatttatgta ccaatatatt gatgactgta tttcgaaaaa tatacatata ggaatcatgt
      661 tattaaatat cgataacttt aaattattta atgatatata tggatatcta gtcggagaaa
      721 atttattaat tactctaact caaaacctat tgcactttat tccagaatca aagggaatag
      781 tcgcttattt aaaaggagaa ggttttttaa ttgtacttcc aaatgtatca aataaagagt
      841 taaataatat tggtgcaact attatagaga gcgcccgaga attaaaaata aaacttccta
      901 atgaaaaaga aatatactcc aatgttacat ttagtatcgg cagcactatc tggaatggtg
      961 aaccaaacat gactactttg atgcttttca accaagtata tagtgcttta aaaaaggcta
     1021 aaagtgaagg taaaaataga tacattctat ttgattttaa agataatagt ttttacaata
     1081 aattagaaaa tgataaggag ttcttatcag aaatatcaca tgttctacaa actaaacaaa
     1141 ttgaagtatt ttaccaacca ctttataata taaaaaataa tactatagtt ggatgtgagg
     1201 cacttgcaag atggagacac cctattaaag gacttcttac tccagataaa tttattcctt
     1261 tatttgaaca gtttggtctg ataacggaat tagatttgca tttatttgaa gagaactgta
     1321 aaaatataag aagatggatt gataacaatg atgtttttgt tccaatttgt tgtaactttt
     1381 ctcgtcttca tttttttaac cctaattttc caagtatgct aaaaggtatt actgaaaagt
     1441 acagcgtctc tacatcaaat tttagcattg aaataacaga aaatatatta atagaaaata
     1501 caaatacaat tattcaacaa ctagctgaac ttcgttctta tggtttttct gtatcaatgg
     1561 acgattttgg ttctggatat tcatcttttg gtatgttgca aaatctacct tttgatataa
     1621 tcaaaattga taggatattt ttatctcata atttaaatga ttttaaaaat acaaccattt
     1681 tgtactctat aataaacatt gcaagagctc ttgggatgct tacagtatgt gaaggtgttg
     1741 aaacagaaca acatgtagaa tttattaaaa gtataaattg tgatatcgcc caaggttatt
     1801 attatgcaaa acccatggag tgcaaagaat ttgaaaaatt actatctaaa gagaataatt
     1861 cagagaaaat gtttataaaa tattctgata taggaaaatc tttaatagag aagatatttg
     1921 atggattttt tattatgcaa gatatgaaaa aactaagagc taatgtcact gaagacattc
     1981 aatggtttga tttttacaac aaaactgaaa ttacatcttt tcctaaagta atagagcatt
     2041 ttgaaaaaca tattaaaggt aaaaaatttt ctattcttta caaaagtata accccacata
     2101 atactgaatc agattcatta ttaattagtg gagaagccgt tttatcagat gaagcttctg
     2161 aacatcaaaa atatagcaat ttctacttta gtgcaaattg tataatttca gaaaataaat
     2221 tactcttagc aaaattacat ttggatttaa ttaaaactgc tggctatata aatgaatttg
     2281 aatcatattc aatacctaaa atgacaaata cacatcttga tgaaagtaat attttatcac
     2341 aattttattc aatgttgcct gtaggtataa ttcgctacga tttatttggt gatatgatta
     2401 ttacttatat gaatgaagaa atgtttgata ttataggtta tacaaaagaa cagttttttg
     2461 gtgagatggg cggaaattta agagcaattg ttcatccaga tgatttagac tttgtttata
     2521 aaaaaagttt ggctatgatt gacacaaata aaattgaacc atttttatat agattcataa
     2581 aacgagatgg tcaaattgtc acagttatgt atacacaatg taacctttct gcaattgatg
     2641 gaaggtcgat tgcccagggt atgtatgtag atgtaaataa aataaaaaat ttaacaaaaa
     2701 tctagtacaa agtattttat tttggagaaa ttaatatgtt taaagaaatt tttttaaaaa
     2761 cttttccagg atttctaatt ataagagatt ctaactacag aattatattt ataaatgata
     2821 atttaaaaaa tttaattaaa agttatatgc aagataatcc acttggaatg actaatatag
     2881 aaatagctaa gaaactccct gataatattg ctaaattttt tatggattct cacaatattc
     2941 aattagattg ggaaaaaaat tatccttatg ataaaatttc aaattggatt tttgagttta
     3001 aaaaagatac tacaagctat tggaatgttt tagaatacaa agtagatgtt gatgaaaaaa
     3061 cctacataat tactatggcc aatgatataa caaaactcta tgaagaaaat aagagaaatc
     3121 ttcactactc aattactgac ccattaactg gagcatataa tagaaaatat ttaaatgata
     3181 gatttgacat atttatagga gattatattg tactaattga cttagataat ttcaaaatga
     3241 taaatgacta tgaaggtcat aatgtaggag acaagatttt atgtgatttt gtttcacttt
     3301 taaataaaga attaataaat tctacttcta ttataagatt aggaggagat gaatttattg
     3361 ttatattctc tagtgatgtt gacaaaaatt atgtttattc acaactagaa gccttaagag
     3421 aaaatttttt aaaagtattc tctaagtata agtatctttc atttagttat ggtgttgata
     3481 cagtaaaaag aaatttaaaa ctgacaatag ttgaattgga taaaaaaatg tataaaaaca
     3541 aggagaaaaa caagaaaaaa tttgataaaa atgattatta atattgttat tttaaaataa
     3601 attaaaatta tctttctttt tttaaacttg tacattagtt tataaatatg tatctataca
     3661 agatattgac attaagtact gaagcatata ttgtttcagt tcttttttgt taaaattaac
     3721 ttatttaaca aagatatcat agtttaacaa agcttttaat ttaaatatat tgatgtataa
     3781 tataattaaa atataatagc aaaaagaaag gaggaatatc atgagcatac tcctaaaaaa
     3841 agcacccaaa ttagcaaaac atattataac aagtttttat ataaatcggg atattgatga
     3901 agtattaaaa tacctttgtg aaaatgttac atggatcgga ccaggagaac aggaatttct
     3961 cacttctttt aatgaaataa aaaattattt ttatgcaggt caagatgaaa ttccttcttg
     4021 tgatataaat aacgatatat ttgaaactgt aagtgaagat gagaatagat gtatggtatt
     4081 aggaagatat actgttcgaa ctaaagaaaa tgctcaaatg attttagagg taaatcaacg
     4141 ttgtacattt gagataattg aggatagagg aaaattatta gtaaagcaca tgcacatatc
     4201 aaacccttat ggtgaaatgc aacttgatga atactttccc actaaaattg gaactcaaag
     4261 ttatgattat ttgcaaagat tactaaagga aaaaacagaa gttattgaaa tgattacaaa
     4321 taatatcaat ggtggcttaa aaggtagcaa tgatgataat acatactcat ttttctatgt
     4381 caatgaagga ttgcccaaaa tattaggata cacatatgat gagttcatgg aaatgagtgg
     4441 tggttcagca gtgggtgctg tatatccacc agacttacct aaagcactag aagattgtga
     4501 acgatgtttt gcaaaaggac ctacatattc atctgaatat agaatccgaa aaaaagatgg
     4561 aacattaatg tgggtattag attctggaat gaaatcgtta aatagtgatg gcatagtaaa
     4621 aattaatagt attattactg acataaccca actaaaaaac atagaatctg aactaaaatt
     4681 agaaagagag cgatatcgta ttgctttgca aaatattaca gatatcatgt ttgagtatga
     4741 tattgaaaac gataccctta taaaatatca acgtgtagaa attgacaaaa aaattgaatt
     4801 agaaaatttt gaaacaaaaa attactccaa actattagaa tctggtaaaa ttattcacct
     4861 tgatgatatt ggaaaactac tagaagtact tcatggaaat cttcgtgaaa caattgagat
     4921 aagacaagta aattcattaa ctaaaaatga atggagatgg attcgtgttc aatgttcagt
     4981 tatatatgat agtgaccata accctatcaa aacaataggc gttttaaaag acataactga
     5041 agataaatct aaattagaaa gcttaattaa tcaggctcaa cgtgacccac taacacaatt
     5101 atataatcaa agagtatcac aaaatcttat tgaagagtat ctatgtagct ctgattccaa
     5161 aaataatgat gctctattag ttattgatat tgatgatttt aaaactgtaa atgatacttt
     5221 tgggcattta gagggaaatg aagttttagt tgctgtatca aaaatactat tacataacac
     5281 acatgataaa gacatagttg ctcgaattgg tggagatgaa tttacaatat ttataaaatc
     5341 attaacaaag gatttaataa taaaaattac taatgatatt ttaaatgatg caagtaaaat
     5401 aaaagtaaag gacaatcata aaataacatt aagtattggt attgccttca ctgatgattc
     5461 aactaaatta tataaagatt tattttctaa agctgataaa gctctttatc tatcaaaagc
     5521 agatggcaaa aattgttata gtacatacga ataataaagc tatttatacg accaagaatg
     5581 aacctttaaa tttaatttca tagtctaaca atcaaagagt tgaagctaat cttatagcta
     5641 gtcaactagt tcctagaaaa aatttagaca atatatttat tgatactgct catttttcta
     5701 aagagcaaat ttatataaaa aattaaactt ttatatacat acagatacta atgataaaca
     5761 gttatcatta gtatcttcta tcttaaaaca aaatgataat ttatctatat aaaaaagtgc
     5821 tttgttgaca gagttcactt ttttatatag gtacagaatt taagtataaa tacatgtttt
     5881 attctataca ttttagttaa tgtaaaattt cttgattgta ctctctaact tttcaacatt
     5941 atactattta tataccactt ggttaatata aaactattat tatagtgtcg tgtggagggc
     6001 ggtgtgactt atttacatac cacttagtta atataaaacg aaggtgttga atttactgtt
     6061 gaaaaaactt ttgatgaatt tacataccac ttagttaata taaaacctag caacagcaag
     6121 agttgcaaca acattcgcaa gttcatttac ataccactta gttaatataa aaccccaaaa
     6181 taaactgggt attttcaata cttacacatg tgcaactctc ttaaatttga agtgaaccat
     6241 gagtagtgca aatgataaag cttatcacat gccctcaaca ccttatattt caagcgttaa
     6301 gccctacttt gtaacaaata tcgctcactg caaaactatt tacattttta ttatatcata
     6361 aatatattat ttttgaatat ctgtaccaat ttgtggtata ataaaaacaa gaagaactac
     6421 aatctattta gacgtagagt gaagttcaaa tactaatgtt tattcttttt gaacttgatt
     6481 tttagttcaa aactaaagtc actcctgcca gagtggcttt ttactttttt aaataattta
     6541 ctatttaagt aaactattaa actggcaact aaacttgcta gtacaccttg taaaaaatta
     6601 tccatacata ctcacctccc ttctatacat tgggagaata atcttttgta tgaactccac
     6661 tctataaatt gtagattaca tcttcttgct aaaaatgtta tagcatataa ttcttacata
     6721 ttttacctat atattacttt tctaaagatt cgtctatata tttctgtata catctactct
     6781 atcctcgatt tgttacaacc ccaatcaaaa aagtagttca acttctaagt tttatacaaa
     6841 gcaggaaact caattattat tagaaaaatc aaaaaatact cctataggta taccaatttt
     6901 tttgatgcta actcttggat tacgttttgg tgaagctgta tcacttatat ggtcagatgt
     6961 agaccttgat aaaaaatctc acatgtaaat caaacattag tttatgtata caataaaata
     7021 atcttcaaag gccctaaaat ccctagattc aaaagaagat tatttgttcc tgatgaactt
     7081 ataattttgc ttaaagggga aaaattaaaa caaaataagt taaaattaca gggtatattg
     7141 aaaaatgaat ttgatttgat atgcttaaat aaaagattca acccgtgagt tcaatcaact
     7201 tttgttgttc attttaaaaa actattaaga gataataatc ctagagatat aaaagtacat
     7261 gaattaatac atgcaaatgc aactttgata ttactttcag gtactaacat aaaagctatt
     7321 tataaaatat tagtacatgc agatataaaa atagctatga atagatattc gatattctca
     7381 cttattagat taaatggata aagaaacatc tgaaaatata agcaaaatac tattcaaata
     7441 atgttcagtt ttatgtcaat taaatgtcac tcaaagtgct ttttttcatt tgtcagttaa
     7501 atgtcactta aagtaccttt ttcctatttg tcagttaaat gtcagtaata tataaataag
     7561 atatatttat aagaccctta aaatcactat tatttaataa aatttataat tatatactat
     7621 ttgctgttga cattggtatt ataccatagt ttatcattta tattagaaat aaaattatta
     7681 attaatagtt tcgtaatttt ataatagatt tacagggagt gataataaat ggatacaaaa
     7741 atatctataa acgaaaacat gagtctaggt aaaagatttt ttaaatacct agcaccatct
     7801 gtagttgcaa tgtgggtttt ctcactatat acaatggttg atggaatatt tgtaagtaaa
     7861 ggagttggag aattagcttt agctgctgtc aatatatcaa tgccttttat taattttata
     7921 tttgcagttt ctctattatt ttcaactggt gcatctacca taattgcaat ctatctgggg
     7981 aaaaaagata ttaaatctgc taacgaagtc ttttcattta acttagtatc aataattata
     8041 ttatcaataa ttatattagc tataactttt tttaatttag atagattagc actattttta
     8101 ggagctacag aatctacaat tggtatggtt aaagattatt taggaataat aatatttttt
     8161 aacggattct ttatagtgtc ttattcttta gaagttataa taaaaactga tggctttcca
     8221 atattagcta ctgttggtgt aataatatct gctcttacaa acatcatact tgattacttg
     8281 tttgtaattg aatttggatg gggtgtaaaa ggtgctggta ttgcaactgg tttgtctcaa
     8341 gtcttttcta ctattttctt cttgatacat tttttaagaa aaaactctac tttgaatttt
     8401 tcaaaattta gaattgattt taagacactt agaaaaattg tatttatagg ttttcctgat
     8461 tcaactactg aattaagttg tggtatagtt gttttacttt ttaacttaag tcttactaaa
     8521 tatattggag aaaatgctct tatttattat agtgtcataa attatataaa tacattagta
     8581 cttatgacta tgatgggtat aactcaaggt atgcaaccac ttactagctt ttattatggt
     8641 gcaggaaata tagataatgt aaaaaaatta ttaaaaatgg ggataaaagc cacaataata
     8701 gcatcagttg ctgtatttgc aatttgtatg gctttttcag gaccaatagt ctctttattt
     8761 atacatccag aagaaacaat gctattcaat gaaggagttc gtgtatttaa aatattctct
     8821 atatctttct tattggtagg aattaatgta ataatttctg gattctttgt atcagtagaa
     8881 aaaccatcaa tttctactgt tatatctctt ggtagagggc ttgtaatagt tgttttaagt
     8941 ctaatttcaa tgatactaat atttggtgga caaggaatat ggatgactac tattgtttct
     9001 gagtttatat gtcttatttt atcattagtt tttctaaaaa agaatttttc tacattagac
     9061 tctaatctaa ataaggttgc ttagatgata agagtgacaa tatatgaaaa tatctaaata
     9121 aaaaatacct attatacaat cttgcaaata ttagaccttt gcaaaaatta ggattatata
     9181 ataggtattt tatttatgta attattttat ttctaaatat ccatagccat cttttattaa
     9241 agttcctttt acttcatatt ctacaggttt tttaaatatt ggaccatcta cgacaaatgt
     9301 atgatactct ccatgttctc cacatgggtc tgcaccagca ttttttatct tctccacaag
     9361 ttctctagtt aatttttttc ctaaaaattc ctctcctaag tgctttaagt ttacttttgt
     9421 tacaacacta caaaatccac tgtctataaa ttcatatact aaatcctctc tactctcttg
     9481 ccataatggt aaatctactt tcataccaac tgcttcacat ctatcagtat cccattttct
     9541 atgactttct atatctatat ctccatatgc acatatcgtt gctcccatgt ttttagcttt
     9601 tattaaagcc ctctcaaagc tctcttcata tccttctaca ccacactcag caactaataa
     9661 aggtaatcct atattgctac ttacttgctt aagcatatta tattctaaat tatgtgtcca
     9721 tgactttcca tgttcttcac ttatagttgt caagagtgcc acaggcttat atcctttttt
     9781 caacattcga tttaatgcta atatactatc cttaccacca ctaaatgaca ttacaaattt
     9841 ttcatcagcc aattttaaca cctctttcat ctaactttgt tttattaaca taattatacc
     9901 aaatttttga gttgcatagc aaattatatt aaattgttaa ttcactataa agaatgtccc
     9961 taatattaaa agtataaccc ctatgtattt gtataaatgc attgtttctt gtcccaaaat
    10021 tacgctatca ataaaaaatc caactaaaac ttgggataca agagttaata taagtgtatt
    10081 tgatactcct aaatttggaa ttgcctttac tgtaaaatat attattagac ctccaaatac
    10141 tcctcctaat aacaacttat aattaaaacc atttatagat actagacact ttaagtcacc
    10201 tgtaactatt atacttgcca aaaaaaatat tgctcctaca gttaagctta taaaggtggc
    10261 cactaatgct gttgtacttt ttcctagctc tccatttata aaagcttcaa gagctgtaga
    10321 accacctgct aataatgcaa atattattgc ccataatttt gtcatggaca aacctccttc
    10381 tatataattc tattaataga agtttatgat acaacctatt aattaattac taagtatagt
    10441 ttaattttta tacactaggc tttatttgga tatgaatttc tgttataatt gttctatcga
    10501 ttttggaggt gattttatgt acacaaattt taatatcgat tttaataact ttaataaaaa
    10561 agaaaatgct attaaattta tgttaatggg agtattactc attatattag gattattatg
    10621 ccttacattt aagacattag gtataaaatt aatttcttgg acttttggta ttgcactttt
    10681 attctttgca tatttaaatt taaaaaatat taatgaacta aaaagatatg ctactaaaga
    10741 agaaattaaa ccctctatta atatccaatg ggttctaata attgcatgta tattactttt
    10801 tgtatttcct caaaaaatac aaagcatatt ttcattattt ttaggatttt atttaatctt
    10861 taatcaacta gtagcacttg taaatagtaa gaataatcct tattctaaat ttacaacttg
    10921 gaatatagtt aagattctat ttggtatatg ccttatatta tctccacttt ttttatctag
    10981 atttatagta tcgataatgt cttttttcat aatattattt ggactagttc tatttttctc
    11041 tggaaataca gcgagaaaat attaattatt ttctcgctgt atttttatat tacttaaaat
    11101 agaatttgtt taagttatct atttgtggta atatgttttt tgtacaagct cagcgtacaa
    11161 ttttattaga aataggtgat ttttttggat aaatttaaat atgcttttga taataaaaga
    11221 tatcatactt ggaattacta tcttagaaat acttttggag aaaaagtttt taaggtatct
    11281 ataaatgcag gatttacttg cccaaacatt gatggaagtc ttggttatgg cggatgtact
    11341 tattgtagca aagaaggctc aggtgatttt gctggaaacc ctaaagataa tcttatcagt
    11401 caattttata atataaaaga aatgatgtta aaaaaatggc ctcatgcaaa gtatattgga
    11461 tactttcaag cctatacaaa tacgtatgct cctcttgaag ttttaaaaga aaaatatgag
    11521 actatattag aacttgaaga tgtcataggt ctttcaatat ccacaagacc tgactgtttg
    11581 ccagatgatg ttgtagaata cttgtctgaa ctaaataaaa gaaccaattt atgggttgaa
    11641 cttgggcttc aaacaattca tgattctaca tctaaaatta taaatagagg tcatgattat
    11701 aaaacatttg ttgaaggtgt tgaaaaatta aagtctaaaa atataaaggt tgtcgtacac
    11761 attataaatg gtcttcctgg tgaagattat aatatgatga tggaaacagc aaaggcagtt
    11821 ggtaaaatgg gtgtagacgg tataaaaatc catctattgc atgttataaa agatactcct
    11881 atggaaaaga tgttacaaaa tggtatgtta actcttatgg aacaagatga atatatcaat
    11941 cttgtctgtg accaattaga aatacttcct gaaactatga taatccatag actcactggt
    12001 gatggtaaga gggatgaact agtaggtcct atatggagcc taaaaaaatg ggaaatttta
    12061 aatcaaattg atgatacttt aaaggctaga aattcttatc agggctgtaa gtttgtttaa
    12121 tacaaaataa taactataga aatggttata taaatatagt cctttctata gttatttagt
    12181 atttacaaaa caaaatatta atttgtatat ttatacaata tataataaaa aatagatagt
    12241 ctaaactgta tctacttata ttttatttgt actgtccact ttattctata aaatttataa
    12301 atagttattt tttaatacca aatataattt ggtataattt ttgttggact ctaaaatgta
    12361 taccaataaa tcctttttaa gtatataatt ctaaatactt tcagtttttt caatctgctt
    12421 taacttgttg gacttataaa atattcttat ttcatcataa gaaagattaa gtgctactac
    12481 tgcagatatt aacatcaaca tcactccaaa taacttccct aaggaaaaac tttctcctaa
    12541 tatagtgcaa ccaattatta ctgaaccaac taattctact gatgccacaa cacctgcaac
    12601 tgacaactct acacctttag atatacctgc tgcataaaaa atatatgcaa gtgcagctgg
    12661 aacaatgcct aacattagca tacaagataa aacatctaaa tccttagcat aatttaagat
    12721 ttcccaaggt cttgaagaag gaatcataaa tatagctcca aataagaaac tgtatactaa
    12781 tattgttgaa cttgaaaatt cctttaaagc atttttacta atagttggca taagtgcata
    12841 tgctattgct gataataccc caagtaaaag tccaaaagcg tttaaacctt gaaaatccaa
    12901 acgtccacca gtcactgcca taattgcacc aataaaacaa agaataagtg aaaataattt
    12961 gcttctagta atattttctt tataacatat ttttgaaaat attgctaaaa acagtggtga
    13021 tgtatatagt aaaactgctg caattgatac acccgcaatg tcaattgctt taaaataaca
    13081 taaattaaac attgcctgac atataatacc tattattact gaatacataa ttcctttttt
    13141 gcttatcttt aagatttgtg gtgtccttat agatgaatat acgcctaaaa ctataaatcc
    13201 aaaaaacaat cttgtaaatg ctacttgttc tgatgtcagt ccatttccca ttaaaaactt
    13261 tccaaataat ccaagtgtag cccataataa ccctgctatt gctataaata catacccttt
    13321 acttttttct cccattttaa attcccttct atttttattt agtcaattta cactcttctt
    13381 tttaagcaaa aactatgcca tcttaaatca atatatatcg actctaaaac cctagtcttt
    13441 ccaataatac taataaattt tcaatttttt tatatttatt tttcgcttac tttaagttga
    13501 tttagactta ttttaattgt aaaatgagtt atatataact tatttaaagt taaaatagac
    13561 tgaaacttat atataaatat caaattttaa gtatattaat caattaaacc tattttaata
    13621 tatcattaaa aatttaggag gaaaatataa aaatgaattt agatttggaa ttttatcaaa
    13681 aaatattaga agcttcccat gatgaaatat gtgtttctga tgataaagga ataataatat
    13741 attgtaataa agcatttgaa gaaaattatg gtctaaaaaa agaagatatt ttaggaaaaa
    13801 atgtttcttt tttagaagac tctggatatt caacaaaaag ccccatacca attgtattaa
    13861 aaacaaagac taaattttca ctggaacaag atactcaaac agggaaaaag cttattataa
    13921 ctgctacccc tatatttgat gaaaatggaa acttagaatt tactgttgaa aattgtagag
    13981 atataactga acttaataat ataaaaaata aacttgaaga cacaaaaaaa caagtaaaaa
    14041 aatataaatc tgaagtagaa acactataca gaactgcact tagaattgag gatactgtaa
    14101 ttatggacgg aatagttatg agaccaatca taaacacagt aaatcatgtt tctaaaacgg
    14161 atgtaagtgt acttttattg ggtgaatctg gaacaggaaa aagctcactt gctagatata
    14221 tacaccacaa cagcaataga tctaatggcc catttataac aataaattgt gcaactatat
    14281 ctccacaact tcttgaatct gaactttttg gatatacttc aggtgctttt acaggtgcaa
    14341 gtactaaagg taaagttggg ctcgtagaat tagctaatgg aggaactctt tttttagatg
    14401 aaataggaga tataccacaa aatctacagg caaaattttt gcaattaata caggatagaa
    14461 cttttactcc agttgggagc ttaaaaaata aaaaggtcga tataagaatt atatctgcta
    14521 caaatgttga tttagtttca aaagtaaaag aaaaaaagtt tagagaagat ttatactata
    14581 gattaaatgt tatagaaata aaattgcctc ctcttagaga aagaagggat aatctagtgg
    14641 aaattataaa atattatttt aataggtact caagtgactt caacctaaat aaaactattt
    14701 ctaaagaagc tatggatgct attgcaaatt atagattccc aggcaatata agagaattac
    14761 aaaatataat tcaaaaaatc ttgcttactt gtacagataa tcatataaca atagatgact
    14821 taccaaacat tcttacaaaa aatatacata ttaccaataa tggaaataaa acacatatat
    14881 cacaaattaa taaggttatt atatctgact caaaatctac taattataaa aataaaaatt
    14941 ttgacacttt aattaaagag tatgaaaaaa atataatttt ggatgcttat gaaaaatttg
    15001 gtagttctta taaagtagcc aaacaccttg aaatctcaca gtctaaagcc aataggttaa
    15061 ttagaaagta tactaatact taattttaaa tttgat
//
LOCUS       DAEMIK010000109        14985 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEMIK010000109 DAEMIK010000000
VERSION     DAEMIK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.109"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(563..1642)
                     /locus_tag="KPK87_002710"
     CDS             complement(563..1642)
                     /locus_tag="KPK87_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2538849.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(1726..2382)
                     /locus_tag="KPK87_002711"
     CDS             complement(1726..2382)
                     /locus_tag="KPK87_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2538850.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            2704..2910
                     /locus_tag="KPK87_002712"
     CDS             2704..2910
                     /locus_tag="KPK87_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538851.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(3063..3491)
                     /locus_tag="KPK87_002713"
     CDS             complement(3063..3491)
                     /locus_tag="KPK87_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2538852.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGDMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(3967..4905)
                     /locus_tag="KPK87_002714"
     CDS             complement(3967..4905)
                     /locus_tag="KPK87_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG2538853.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            5791..6150
                     /locus_tag="KPK87_002715"
     CDS             5791..6150
                     /locus_tag="KPK87_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538854.1"
                     /translation="MRKKKDTHSFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGKHPSFHLFHDLVTMFNISVDEYFYPAKNVEKSTVSRQIDSSLDLLSDDELK
                     IIQGTIDGILNSRENKK"
     gene            complement(6248..6459)
                     /locus_tag="KPK87_002716"
                     /pseudo
     CDS             complement(6248..6459)
                     /locus_tag="KPK87_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6572..7894)
                     /locus_tag="KPK87_002717"
     CDS             complement(6572..7894)
                     /locus_tag="KPK87_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2538855.1"
                     /translation="MNQKNENYWLLDAPVTKAIWHMAIPMMLGMSINIIYNITDTFFI
                     GRLNDTAALATISLLLPFTTILMAIGNLFGTGGSTLFSRLLGSENTDRTKQCSATTLW
                     LSFLFGLLTAIISIIFSNYIIRLLGADSNTFAYVKQYLIFYGMGAPFIIANFTLEQLI
                     RGDGKSVESMIGMMISIGANIILDPILMFGLQLGIRGAAIATVIGNAFAVIYYIVCIQ
                     RADNQLSALPKYFRLEKQMLKEIFLVGLSAMLLDILLIVSSLMFNYYALKYGDYVLAG
                     FGISQKLVQIVDLIGMGLYMGVIPLIATAYGARNELRVKEIIKKTALYLALVITCLFT
                     ILFTCRNFIVHCFSNDSDVIRIGAYILTVQLCSSFFAAGAGLLTGIFQAKGEGTPAVV
                     MSVMRGIMLIPAIIFGNYLFKMNGVIFSLLVAEAISCITGLVLYKLKK"
     gene            complement(7983..8636)
                     /locus_tag="KPK87_002718"
     CDS             complement(7983..8636)
                     /locus_tag="KPK87_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2538856.1"
                     /translation="MNNIYSDIYEKLSTLQWIMKRHQMFCQAESGPFADTSRGQGRIL
                     AMLKIQPEIATKELAYLLGIRQQSLNELLNKMEKNGYVERKPSENDKRVMIVHLTEKG
                     KQVQQPKTDYQNIFNCLLPEELLQMSQYLDRIIEAFQLQNGNALEENNMIDWMAQARE
                     RMGDEHFEQLMSMRERAFGHMRPPKDIPGAERFSENYNGYVPDRDGFQPRNFKPDIK"
     gene            complement(8773..9184)
                     /locus_tag="KPK87_002719"
                     /pseudo
     CDS             complement(8773..9184)
                     /locus_tag="KPK87_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(9183..10274)
                     /locus_tag="KPK87_002720"
                     /pseudo
     CDS             complement(9183..10274)
                     /locus_tag="KPK87_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
     gene            complement(10822..11634)
                     /locus_tag="KPK87_002721"
     CDS             complement(10822..11634)
                     /locus_tag="KPK87_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2538857.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            complement(11660..12289)
                     /locus_tag="KPK87_002722"
     CDS             complement(11660..12289)
                     /locus_tag="KPK87_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2538858.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            complement(12633..14288)
                     /gene="ilvD"
                     /locus_tag="KPK87_002723"
     CDS             complement(12633..14288)
                     /gene="ilvD"
                     /locus_tag="KPK87_002723"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG2538859.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(14612..14911)
                     /locus_tag="KPK87_002724"
     CDS             complement(14612..14911)
                     /locus_tag="KPK87_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538860.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT         4604 a         2903 c         2092 g         5386 t
ORIGIN      
        1 tctattatat agaaagtaat aaatcttttt cttaataaaa ttcaatagta agtggaataa
       61 aattttatat gaataagaac attataaaaa tactttatac aatagtcaat aaaagatatt
      121 agcttcaaat tatgttaaag ttgtattatt taaagtgaaa aaacaaataa aaaagaaata
      181 atagtataag tagaaaaata tacttaaaac aaggcgaagc ctctcttgta tctaaatcaa
      241 gtagagaatt aaatctaaaa aagaaaataa atataaaaga tgactcctgt acattacaag
      301 aatcatctta tggtttcata atcactccat ggtttttaac tgtctgatat aaaggtctca
      361 gttcactcat agcatgacca ccttttccga ttgctgaccc cgtcacttat ggacaaaatg
      421 tccataagtg atagtcctct cttaaaaccc tctaaataga tatatctcaa gcaccgcatt
      481 gaaaccagtt gctggaaaca aactgaagcg ttctggaata gtaaaagaaa ttgccaccat
      541 atccacgtta ctgtcctagt tgttactttg ctaacttcgg agcgatttcc tctaacaata
      601 cgcagtcaaa ttctgccttt ttgctaagaa atggcagatg gtctgcatct ggtataaaaa
      661 aaatctgttt gtctggcgcc actatttcat caaaaaattt cttcgctgga agataggggc
      721 tattatagtc attttctccc atgatgtagt atacagggac ctgatagtca actccataat
      781 ttcttatgtc agcttcttcc actaaaaacc gcaatacatc accttgattg tcaatcgcat
      841 ttggattgaa aatggccgta ttttttaact ctctcaatga ataatacggg gttgccagta
      901 tactgattat attgcccaaa tccatattta tagaaagccc atacttgctt tggtatgttc
      961 ttactgtcat aagagctgcc aaatagcttg gaccatattt tgctggcgga tatggtgcaa
     1021 tagcttccag cgccttgata tctttttgct tccctgcagc tttcaccatt tcaagtgtct
     1081 tttcatagcc cacgcgctca ttgtccacca tattaacggt ctgcgcaatc ccaatgtacg
     1141 cgctgtaatc ctccggatag gtttgcacag ccatagttcc cagaactgtt ccccagctat
     1201 gacctataag gataattttc tccttgttga actcttgctt caaatatgct gtcacctcat
     1261 ggacgtcttg cagaacacgc tccgcgctca tggtctgcaa aactgctgcc ggatcgttcg
     1321 caaagtatgt tttgcctacg tttcgctgat cccagttcac cacagtaaaa tcattttccc
     1381 actcatactg gtacagatgt aaaaatggca tcattgaatt accaggaccc ccatgaaccc
     1441 aaaggatgat ggggttgtct gcgttttgtc cccggatata taacgcctgc tgtatgccgc
     1501 cgatctccac catttccatg cggtcgattc caggagcttc tattttatgg cttgtacgta
     1561 taatcgtggg ggctatcaga cgcaaaccgg caaacgccag aacaaaaaca gcaactagta
     1621 tgcaaagtgt gattaatatc attttaagta ccttctttca tgaatttttt tagccataga
     1681 tatctcctct aacattaaaa aaaatatgtt gctattttag ttttgttact ctacctgtcc
     1741 cattggtttc ctttcataaa aaatattaat tgattctata atctcatctt ccaggatatc
     1801 aactcctatt tgtctcataa gaaactgtaa gaattgaaac cgtttacgaa gtgtaggaaa
     1861 gtccccctga aatacatctg tatcacacca aaagttaaag agcagcaaaa ttacctctgc
     1921 gcattcatct gggaactctg tgatgatgct tctatcctct atgccttctc gtaaaacatt
     1981 tgctaatatt ggtgctagtt ttttcaaatt atcctgtgta aatgccagta tgatatgcgg
     2041 actttctatc gcgcttgtaa tcatgttact agattctttt atcacctcat ccgttaaatg
     2101 ccgcctaact aaacttctaa gcttctcttt tgctgtaagt ccatgcattt caccgagcca
     2161 ttgatttact gtctctatta tctgttcaaa gcgcctttcc atgaccacat taaaaatatc
     2221 ttcttttgaa ctgaagtgat gaaaaattcc gccctttgac attcctgaag catcaacaat
     2281 attttgcata cttgttttgt catatccttt ttcagcaaac aactttgcag aaatggtaat
     2341 gatattttca atagcttgct ttggattgtt ctttctagtc attatataac tccttcttat
     2401 taaaaaccga ctgttggttt ttattatata agctattcct gcttctgtca agtaacattg
     2461 tgtttatggc aaaacaataa ggtttggtgg ttagacaggt attaattaga ctaatgcgac
     2521 tttcccctag atggaaccct tggtaaattt gcaatcttta ccggctttga ttatatctgt
     2581 aagcaaggca ggtatggcca cacctgccct gtttgcccct cggatattca ttcctatccc
     2641 tcctctcctt agccattaca ccaaaacgcc gctcattgtc tccaatggcg gcgttttaaa
     2701 gatatgattt ctcatgctgc tgaaatatgc aaaaaatata agatgccctt tattattttt
     2761 cctacaatag aaaagaaaag tattgaagaa tgggttaata ttggagcaaa tatgctttgt
     2821 ttggcacaag atactacctt tatcacttat gttatcaatc aattattatc tttttataat
     2881 gagattgtac caattcaaaa acaacattaa attataaatc taattttata ttaaaagaga
     2941 ctaatatttt atctatctct attaaggata ttttcttatg tatcataata ttagtctctc
     3001 ttattattat atactgctat catcattagt tgctatttat actattttta taatatacaa
     3061 atttattcag ctaacgtttt gcctaattct atacactcat cttttgcttc ttcatttggg
     3121 tcttccattg ctgtaattcc ttctccacca ataattgtag caccagcatt ttgcatacgt
     3181 tcttcccaat ctctcatcca ttcacaattt ccccatccat atgaaccaaa taatccaatc
     3241 tgtttacctg ataccataga ttctaattct gaaacaaatg gctccatatc cccctcttct
     3301 agttgctctg ctcccataga tgggcaacct aatgcaaatt tatcttcttc ttttaaatca
     3361 cttggtttca gtaagctcac atctaaaact tctggtgtct taccttttaa cttcacacct
     3421 tcagccacaa aatttgccat tttttcagta tttcctgttc cactccaata aacaatatat
     3481 attttactca tttttacctc ctgtagtatt ttttaattaa tcaatagtta gttatagcta
     3541 actaacatct aaaaaacaaa agatttcttt atctgaaatc tctttatttt tctaaatata
     3601 gaatatcaca attgattttg aaattcaata cttattgata acatttatca tttatgcttt
     3661 atattcattt tataatatca aaacactgta tcgtatttct aatctttcat agaaaaattt
     3721 ctaacttata gtacttttgc atctcttata aatcttttat atgtataagt acatagaaaa
     3781 acacaccttt tccagatgtg ctatctataa tatcgtatat ttatttttca attctaacta
     3841 gctaatatgt ttataaattt tatcacttaa tacctttata cattgatgta aaatcttaca
     3901 cctaagtaat ttttattatt tgtctcgact ctatataata tagctatttt atcatcaaat
     3961 caaaaattat cttttgctat tattcttgta tttataaaga tttaaatata agtatccatt
     4021 tccttgttcc gttctaggta ttccaagcgc tctcgtacat tttattaatt gtgcatacag
     4081 ttcagcttca tctaaatctc tttcaaactc ctctcttgcc cattctttaa ttagtgctaa
     4141 tgagcctgat acgtatggtg cactcatact agtaccactc ataatagcaa gtttattatc
     4201 catataagtc gatataatat ttcttcctgg agccaccaaa tctattgtag tatttgaatt
     4261 actaaacttt tcaaccaaat agttttcatt tattgcacct acttctatta cctcagcata
     4321 actggctgga taactatact cacttgtact agaatcacca tctccattat tacctgctgc
     4381 acacactaca gaaatattat tttttactgc ttgcatgaca gcatttttta aattcttatc
     4441 atctttgttt cccccaagag acatagatat aatatcaacc ttgttattta cagcaaagtt
     4501 aatagcatta attatacttt gatatgttcc ggtaccatct ttatttaatg cttttgctat
     4561 taataattta caatctggag ctactcccat aacttcatta ttataattag atgcagctat
     4621 aatacccgcc acatgagttc catgaccatt aaaatcctca tatatatttt tatttccatt
     4681 actgtcatca ctaaaatttg caccaccaat tatttttcct tttaaaagag gatgagatat
     4741 atcacaacct gtatctatta taccaactac aatatttttg ccagtataac cttcatccca
     4801 cattccccta gcattcattt gttttattcc atatgggaat ttattttttg cacctcttag
     4861 attttcattt atttcatatg gaattagttt cattctttta ttcatcatat atcctcctaa
     4921 aaattttatt gcttataaaa atcatgaatt ttattttata ataggtttat aaatcaaatt
     4981 aaaacctata tctacttatt tatatttatg atatattttt tatttttagg agtcctattt
     5041 tattgtatat aaaataatat ttagataact atattaaaaa atacatgttt gcttatatac
     5101 aaaacttact aaaatgtctt taatattttt gtatacaaac attatatttt tattataaaa
     5161 atacactaaa tttctgatta tcttttcata agttggtaaa tgccgatata tcctgtattt
     5221 ttcaagttct tcatcaagca cacggatttc cgtactgcac acatcgaatg tcgtatagtc
     5281 actttcgtaa tcatcccaga caatcaattt ctcaataaca atttctggaa gctcacaaaa
     5341 gggtacttcc tttgcctgtc atctggctaa ttccttgcgg tagttacgga cactgccttt
     5401 cacaaccttt ttgagcttac agtccaactg acactgtatc gtcttttgga agtctgatgg
     5461 tttcaaaatc ataccccctt tctctatgaa gtgaaaggta tgtatccctc tttctgcacc
     5521 atatctgtac agcagggtgt gatttgttgc agaattgaaa aactttttga aaatatgaaa
     5581 acagcctatc tgcactaata acaaatagac tgtatttaaa acatctgata tatgagtttt
     5641 taattcatgc tcaagggctg atgataaact aaaaaaaagg aataaattcg gttgtcggtg
     5701 ccggctgcct gttttactta gaatcacaaa ttgctccttg agcagagcaa acctgtgaaa
     5761 atagtataaa ctcatgctag gtgattaaaa atgagaaaaa agaaagatac gcacagcttt
     5821 gattttcgtc ctttaggact tgcaatcaga gaagccagag agaaagcagg gctttccaga
     5881 aatgatttag gcgataaagt cttttacgga gaacgtcata tcgcagatat tgaaaatatc
     5941 gggaaacacc ctagctttca tttattccat gatttagtta ccatgttcaa tatatctgtt
     6001 gacgagtatt tctatccagc gaagaatgtg gaaaaaagca cagttagccg tcagatagat
     6061 tcgtcacttg atttattgag tgatgacgaa ttaaaaatca ttcaaggtac gattgatggt
     6121 atcttgaaca gcagagagaa taagaagtga ttcgcttctt attttctctg tttttctgtg
     6181 cagattcttt aacgcttatt tgttgatata ctccgcaggc aacaaatagt cgtaaatcaa
     6241 acttttgttt ttttgtaatc ttgtaaatat attcttctgg tgtaggacgt ttattgccaa
     6301 aatacttttt gaaatttgcc tgtacctctg gggctggact gttcatgtct tcatcaatag
     6361 tggcttctat attagcccga atatcagcta atgatacatc tccagctcta tcaccagttt
     6421 tcaacatttt tttatatttc tttttttaag cccaatcata tttttccagc tcctaccaat
     6481 ctatactcat tctatagaaa tctcttaatt tattgttgat gatttttata accatcacta
     6541 actttgttaa caagtctgat ggagttcagc attacttttt caatttatat aatacaagcc
     6601 ctgtaatgca tgaaatagct tcggctacaa gcagagaaaa aataactcca ttcattttaa
     6661 agagataatt gccgaagata attgctggta tgagcataat acctctcata acggacataa
     6721 caacagccgg agttccttct ccttttgcct gaaaaattcc cgttagaaga cccgctcctg
     6781 ccgcaaagaa agaagaacag agttgaacgg ttaagatgta cgcacctata cgaattacat
     6841 ctgaatcatt tgaaaaacaa tgaacaataa agtttctgca tgtaaataga atagtaaaca
     6901 aacatgtaat tactagtgct aaatagagag cagtcttttt aatgatttct ttcacacgaa
     6961 gttcatttct tgcaccatag gcagtagcaa taagtggaat tactcccatg taaagtccca
     7021 tgccgattag gtcgacaatc tgcacaagtt tttgagaaat cccaaatccg gcaagcacat
     7081 aatctccata ttttagtgca tagtaattaa acataaggct tgaaacaatc aaaagaatat
     7141 ctaacaacat tgcagataat ccaactaaaa aaatttcttt tagcatttgt ttttcaagcc
     7201 tgaaatattt tggaagagca gataactgat tgtctgctcg ttgtatacag acaatataat
     7261 agataacagc gaaagcattt ccgattactg tagcaatggc tgccccacga ataccaagct
     7321 gtaatccaaa catcagaatt gggtcaagaa tgatattagc accaatgctt atcatcattc
     7381 caatcataga ttctacagat ttaccgtcac ctctaattaa ctgttctagc gtaaaattgg
     7441 caataataaa cggagcaccc attccataaa aaataagata ctgtttgaca taggcaaaag
     7501 tattactatc agcaccgaga agtcggataa tatagttact gaaaatgatg gatattattg
     7561 cagtcaatag tccaaacagg aaagatagcc ataatgttgt agctgaacat tgttttgtcc
     7621 tgtccgtatt ttcacttcct aacagtcgtg aaaacaaagt gcttccacct gttccaaaca
     7681 aattcccaat tgccattaaa atggtcgtga aaggtaacag cagtgagatt gtggcaagag
     7741 ccgctgtgtc atttaatctg cctatgaaga atgtatctgt aatattgtaa ataatgttta
     7801 ttgacattcc aagcatcatt ggaatcgcca tatgccatat agcttttgtg acaggtgcgt
     7861 ctaaaagcca gtaattttca tttttctggt tcattttgtt cgtctccttt tcaaaaaata
     7921 agccctatgg agttcaaaaa atctccatag ggcttattgc tgtaatttta cagtgccttt
     7981 tgttatttga tatctggttt aaagtttctt ggctgaaatc cgtctctatc tggaacatat
     8041 ccgttatagt tttcagaaaa gcgttcagct cccggtatat ctttgggtgg tctcatatgt
     8101 ccaaaagctc tttcacgcat agacattaac tgttcaaaat gctcatcgcc catacgttct
     8161 cttgcctgag ccatccagtc aatcatattg ttttcttcta aagcatttcc attttgcaat
     8221 tgaaaagctt caataatacg atctaaatat tgcgacattt gtagcaattc ttctggaagt
     8281 agacaattaa agatattttg atagtctgtt ttcggttgct gaacttgctt tcccttttct
     8341 gtcaaatgaa caatcataac gcgtttatca ttttcagatg gttttcgttc tacatagcca
     8401 tttttctcca ttttattaag caactcattt agggattgtt ggcgtattcc caataaatat
     8461 gccaactctt ttgttgcaat ttctggctga atttttaaca tagcaagaat tcttccttgt
     8521 cctcgtgatg tatcagcaaa tggaccagac tctgcctgac aaaacatctg atggcgtttc
     8581 ataatccatt gcaaggttga tagtttctca tagatatcag aataaatatt attcatagta
     8641 tgacctcctt ttcaacaggt acctttaaca aataatatac aggtaccttc ccaaaaagtc
     8701 aatagggtac atgtatattt ttaaaactta gtatatgtat ttgaaattgt catagctggt
     8761 ctgctgtaat atttatccta caattttcca attatcgtcc ttgtgaagca caagctcata
     8821 ctgtgagatt tgtgtcattt tcgacttgtt atccagatat ttcacggaca cactgacctt
     8881 gagattatcg ccatctttgg taaagacagg atttaccagt tccgaaaata catagtcgcc
     8941 agaaacagga gcaagcacac cgtctttgac ataataggca agttcttttt ctgtagctgt
     9001 cggatagagt ttaaagaatg tttccaagaa agtagtagcg tctttgaacg tatcggagct
     9061 gacgctgaca tctgctccct gtgctttcgg ttcatacttt gatttctgta ccaccggagc
     9121 aagggtagga ttctgggtaa tgacccattt caccatctgt atacacatga acggtcacgg
     9181 tgtaattttc tgtgactgcc agtttctaag ccatcaagtg tgactttgtc cacgatcttg
     9241 atgtcttttc cggcaacaat cattttttcg cctgttttct tgtcggtagc tgtggtatgg
     9301 attgtgattt ctttttcata ttcatcagta agtgtcccta aatctacggt cacttttttt
     9361 ctggatacga ccacctcaaa agctggaatc agcttaaatc cggcgttgga atcacacttt
     9421 aattcctcaa tcacataggt aatgcacctt tactgtcgtc tggttcagaa gtaccaaacc
     9481 atacaccatc ctcactggat tttccagcat ttgtatttac cttacggaaa gcccaacttg
     9541 aagcagtaga gaactgaccg tttttatctg tgactacaat atgggattcg ccaaatgttg
     9601 ccccctcaaa ggaataattg acgttgccat ctgtagaggg tggaattgga agaagtcttt
     9661 ttgacctttt catttcccac attcaacttc gcccagaact gtccaatgtc ctgtccttcg
     9721 ccagtgtaga tgtaaccgcc acattcatag catcccttat ttgctttcac aaatactttc
     9781 gcaccagcgt aaactttatt ctgtaccgcc tgtgagattt cattatagga agctctgacg
     9841 ttatcacatt gccagccgag ctgttcactc aatctctgcc agacaacaca ctgttccaat
     9901 aagtaaccct gcttgtaatt caagtttgta tgagaagcag tatattgctt gatgtactct
     9961 aaggaaagag caatgtccgc aatctgatcg ctactcatat gggagcttgc gtcatacctt
    10021 gttttatatc catttttgaa atttgtattg atgtccacgc aataggcagt ttcgccctca
    10081 actttcatgt gtccctcatt taatgtggat ttgatagaac catcattcat aacatgttca
    10141 atgtagccga cacgttctgc tgattctgtc cagtattgat tgccagaagc atgaaccacc
    10201 gtcacaggca atgcggtaag aatggttgca atggcgagcg tccctgtcag caatcgcttt
    10261 actagcttat tcatattttc tctttctcct ttcgattttg ggtatgaaaa aaagacgttc
    10321 tgaaaagaac atctggaaat agaaaagaaa cgccaaaagt gtacgttcca tagtctatat
    10381 aagtattcaa tttttactga tgtgccatgc catatctttg catatctggg aaataagacg
    10441 tatcaaggct cattcattag tagtaaattc gtagtaatat cacgatttat ttcctattga
    10501 atatgctttt agatacagtg tttatgggga taataagatt tttagggcgt tttttatata
    10561 aaaatctcat aaaaattttg attatatttt cataagttaa caaatgtggg tataatatga
    10621 atttacaaaa ttcctctgtt tgtataagtg ctttaccaat acaccaatat ataatcacat
    10681 tgaagctata tttgtattta tagtagataa tgtatcaaaa attggttatg aatagtaaaa
    10741 ataccagtta agcatatttt catgtttcta tttttaacat taaatttcca atcttcatat
    10801 gtatattttt aacaaaggtg tttacttatt taaaaactct aaaactcttt cattaaaatc
    10861 ctgtgcttct tcatatgtag cgtgtcccaa tccctcatag ataaatattt cgcttttttt
    10921 aattttttct gctaaatgaa aagaagcatt atttccaaca attttatcat tagcacctcc
    10981 tataattaag gttggacatg ttattttatt taattcggaa aaggcattat gttctatgca
    11041 agaggtcgct tgaataataa atcgtttaaa atcctttggt ttacctactt ttcctaaaaa
    11101 aggaataaat aattggtatt tttttaaata tctttcagaa taggatttct ttgctgtgtc
    11161 aatcattaaa tcattgtaat tctgcttctt cgccatatca atccaactac aaataacatt
    11221 ttggatagta tcattttgct ttgaagaagt aactgccaac acaagtttct caactaactc
    11281 aggataatca attgccagat attgagctat cattccacct tgagaaacac ccattacttc
    11341 tgctttcata attccaagtt ttttcattat atctgcttgg tctttagcca tatcccttgt
    11401 ggaatatttt tctgtaattt gattttttct actaaataca taaaccttat aatcttttgt
    11461 aaattgctta tatctaaaag caaaagcaat cgcctgtatt tttccatgta aaggaaataa
    11521 accatctcct aatcctggta aaataattag tgtcttctta ccactaccaa attcaatata
    11581 attcactgta ttacttccta tcatcatttg acagcttttt gcattataaa acatagtcta
    11641 ttcctcactt ttcctattat cattgtttaa ataaatcttt aaatttttga ataccgtact
    11701 catcaattag aggaatatcc atttttttta acatgaataa taaaaaatcc aacctattac
    11761 atacttcact gtattcacgt ttaaataata cagggtcaag taaaatatca atactaaaaa
    11821 cgagcatttc agctaattcc tctggatatt cacaagaaaa ttctttattt tcaacacctt
    11881 gtttaattat cttagcaata ttgggtgcaa ctacattgtt gagattacga acttcctcta
    11941 ttaaagcgaa tgggattttt tctaaccagc tacaatgtac catttttcta cgtgtgtaat
    12001 cattcgcact atgaacaacc aattctatta ttttttcatt tgctgtaagg gtgtcatcag
    12061 caacaagatg ctctaaaaaa ttattcattt gtgagtaaag ttcttttgtg acacttctca
    12121 gtatatcttc tttagtttta aagtgatgat acattgcacc tctagataga ccagataaat
    12181 tgataatatc ttgtgtagat gttgaatcat atcctttaga cgaaaatagt gaaaatgctg
    12241 attggataat tttttcttta ctatttttac ttttttcttc tctattcata tttccctcct
    12301 aaaaacagac tgtctgtttg ttttttatta tacattttaa tatttattat gtcaagaatt
    12361 aactgacttt caatttcttc atagttcaga tggaacgaaa agcattccac atcaaacttt
    12421 taaaaagttg tattacctca acaaggtcta aaagatacat ctctatgctg tacttgtatt
    12481 ccttataata gagatgtatt gttttcaaag ataatcaaga ttttgctatg tttttcatac
    12541 aaaataaaaa ggatacctca ttagtgctat aacacctaag atatccaatt aatattttct
    12601 taactttttc taatttacat aaatatagtc tactatttta aaactgctcc tgtatttgct
    12661 gatgttacca gtttagcata tctacttaag tatccatgct ttattttcgg ttctaaaggt
    12721 ttaaatttta attttctatt ttctatttca acttcatcta cttttaactc taattttttg
    12781 tctggtatgt ttatagaaat tatatctcct tcttcaacta gtccgattaa accaccttcc
    12841 atagcttctg gagaaatatg gcctatggat gcccctctag ttgcccctga aaaacgacca
    12901 tcagtaagaa gtgctacatg cttatcaagt cccattcctg caactgctga tgtaggagat
    12961 agcatttctt tcattcctgg accacccttt ggaccttcat atcttataac tataacatca
    13021 cctttgttta tttttttacc aaaaatagca tttacagctt cttcttctga attaaaaact
    13081 cttgctggtc cttcatgaac taacatttct tctgcaactg ctgacttttt tacaacagct
    13141 ccatttagag caagatttcc tttcaatatt gcaagcccac cttggttact atatggattc
    13201 tttaatgtat gtattacttc ttcattttct atttcacaat ttcttatatt ttcccctata
    13261 gtcttgcctg ttacggtttt gcaatctaaa tttattccat ttatctttga aagttcgttc
    13321 attatagctg gtattcctcc tgccatatgt aaatcttcaa tatgatgttt tccacttgga
    13381 cttaattttg ttaaacaagg agttttttca cttatttcat caaaaaaatc taagttaagc
    13441 tctattcctg attcataagc tattgcaggt aagtgaagta ctgtatttgt agaaccagcc
    13501 attgccatat ccacagctat agcattttta aaagcatcta ttgttaaaat atctctaggt
    13561 tttatgtcgt tcttaagtaa ctccataaca tacatacctg catattttgc tatccttttt
    13621 ctttctccag aatgtgacgc agcagtacca ttatatggta tacccatgcc taaagcttct
    13681 gttaaacaat tcatgctatt tgctgtgaaa agaccagaac atgaaccaca tccaggacaa
    13741 gagttcatgg ctaactcttc taattcttct tcacttactg ttccatcttt acatgctcca
    13801 acaccctcca ttgcagagtt aaaatcatat actttcccat tttttttacc tggaagcata
    13861 ggccctccac tcacaacaat acttggtata ttaagtcttg cagcagccat taacatacct
    13921 ggcactatct tgtcacaatt aggtataagt accaaagcat caaaaccatg tgccatagcc
    13981 atagcttcta ttgaatctgc tattaattct cttgaagcca gggaataatt cattccgaca
    14041 tgccccatag ctataccatc acatacccct atagcaggaa attctaatgg caaaccacca
    14101 ctcattctta ctccattttt tgcagcttct gctatttcat caagatgcag atgaccagga
    14161 atcacctcat tttgtgcatt tactatacct attaatggtc tttcaatttc ttctttagta
    14221 agacccattc catacattaa cgctctcttt ggagctcctt ctattccttt ttttatatca
    14281 cttctcataa tattacctct ttcttacttt tttaaatttt cataatatta ttataatata
    14341 tttttctatt aattaattac gaaaattgta aaaaaatatt agcatatact aatgaaaatt
    14401 tattacgatt ttttatcaat atcttacaca atcgtattat ataatattat cttttcaata
    14461 tattttataa actaatatat tttacaaatt gttgagtaat attttatgat ataaatcaga
    14521 agaggcactt ctataataga aagtgctcaa ctatgaataa gattaataat atatttgcct
    14581 tagatatata gacttttata aatattaaag attaatcaaa tctgatgttt gaaactttat
    14641 cccaattaat cttccttgaa tagtcttctg gatgtgataa tctaactata tgagttgaat
    14701 tatcatagct gtaataaatt gtagtagtac catcaactgg attctttttg tatccttgat
    14761 acacagcact atttggtact ttcttttcaa caaagctata tgcagatgaa gtatcaatag
    14821 tttctatttt attaggaaat ataaacatat aaaaagctac tactataaaa atacatatgc
    14881 atgtaatact ataccattta atccatttca taaaattcct cctactttac caaaatatac
    14941 tattattatt tccatatatt tataatattt atgcatacga taaaa
//
LOCUS       DAEMIK010000110        14674 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEMIK010000110 DAEMIK010000000
VERSION     DAEMIK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.110"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            59..964
                     /locus_tag="KPK87_002725"
     CDS             59..964
                     /locus_tag="KPK87_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538861.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            1079..1259
                     /gene="ssrS"
                     /locus_tag="KPK87_002726"
     ncRNA           1079..1259
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPK87_002726"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1442..2659
                     /gene="cdeC"
                     /locus_tag="KPK87_002727"
     CDS             1442..2659
                     /gene="cdeC"
                     /locus_tag="KPK87_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG2538862.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(2759..4081)
                     /locus_tag="KPK87_002728"
     CDS             complement(2759..4081)
                     /locus_tag="KPK87_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2538863.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(4132..4926)
                     /locus_tag="KPK87_002729"
     CDS             complement(4132..4926)
                     /locus_tag="KPK87_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG2538864.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKNKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLRGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            5036..6481
                     /locus_tag="KPK87_002730"
     CDS             5036..6481
                     /locus_tag="KPK87_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2538865.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            6510..6719
                     /locus_tag="KPK87_002731"
     CDS             6510..6719
                     /locus_tag="KPK87_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG2538866.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            6739..8133
                     /locus_tag="KPK87_002732"
     CDS             6739..8133
                     /locus_tag="KPK87_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2538867.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            8373..9125
                     /locus_tag="KPK87_002733"
     CDS             8373..9125
                     /locus_tag="KPK87_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2538868.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            9151..9564
                     /locus_tag="KPK87_002734"
     CDS             9151..9564
                     /locus_tag="KPK87_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2538869.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            9568..10311
                     /locus_tag="KPK87_002735"
     CDS             9568..10311
                     /locus_tag="KPK87_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG2538870.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            10311..10781
                     /locus_tag="KPK87_002736"
     CDS             10311..10781
                     /locus_tag="KPK87_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2538871.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            10798..11556
                     /locus_tag="KPK87_002737"
     CDS             10798..11556
                     /locus_tag="KPK87_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2538872.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            11540..12382
                     /locus_tag="KPK87_002738"
                     /pseudo
     CDS             11540..12382
                     /locus_tag="KPK87_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(12542..13447)
                     /locus_tag="KPK87_002739"
     CDS             complement(12542..13447)
                     /locus_tag="KPK87_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2538873.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            13809..14543
                     /locus_tag="KPK87_002740"
     CDS             13809..14543
                     /locus_tag="KPK87_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538874.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT         5819 a         1631 c         2547 g         4677 t
ORIGIN      
        1 aaaatatgaa aaacatactt taatataggg caattatata atataagggg gaattgtctt
       61 gaataactta ctatttttga ttggagtgat tgcattgagt aatggtaata tctctatatt
      121 tgatgataaa aataaatcaa aaggaaataa tagagttaga aaaaacaaaa aaaatgcaaa
      181 tgataaactt tcacagtcta cacatggcaa tttaaggttt gatgcaaatg atataaaaag
      241 aggtttaagg ctcatggatt taagtgaaga agatttagaa atgggaatgg aaataataac
      301 aagaactaaa aaatacatgt caagagatga aagaaaaata ctaataaaaa tagaaagtgt
      361 tttagattta gttagaggaa taaaaaaatt aaataatata gatatggtag atacagaaga
      421 tgaaacggat tttttcagaa aaatggatag tgatgataaa aagaatatga tgataaagga
      481 aattatagat gtattcccag aaaaaagaaa aaattcagtt gaaaaagcta taggaatgaa
      541 aaagaagata gatttatttg cagaattatt tttaccagat gactttggtg aaggtggatt
      601 tagtttgagc tctttggcaa atataaacaa tcttggtagt atgaataatc tcaaattgtt
      661 aggaagtctt ttaagaggtg atgatagtaa taatgatgat gaagacaata atgatgagtt
      721 aaattatgaa gacgaagaat tagattgtga agacaaagaa ttggatgatg atgagttaac
      781 agatgaaggt gaaatggagg ataatgaaga tgaattagta gaagataaga acacaggcta
      841 tgaaaatata gaaaatgatg aagatatagt atacgatgag gaactataca acattaatga
      901 gcaagaatcc gaatacaatt ataatgaaaa aaatactaaa actagagcta agaaaaaacg
      961 ataattaact aaatacataa agaaaaaagg aagaaaaagt aatgatagaa taagattatc
     1021 tcaattacta tataaaacca ctttaaaaaa ttcaaaattt attatataat aataatatga
     1081 aatcctgaaa catacgataa ggctttttta aattcaacct acaaatgaaa tacgggagac
     1141 cgtgtctggt aaattataac atgcctagtt agtttggacg ttaaaggtta ctggcaagtc
     1201 acccacctgg gagtgcctag ggtgtgaatt tacttagcca ccggtgtttc gggacaaaaa
     1261 aagtgctact atttcaaata gtagtacttt ttttattaat atgcattttt ctactaaata
     1321 cgcctaatta ttgataaatt caaaaagcaa gtaacaatta agattataaa agcatagtat
     1381 taattaggct tcaaaaagcc aaagacttaa taagaatcgc ttacagggag ggcaatgaaa
     1441 aatgcaagat tataaaaaaa ataaaagaag aatgatgaat cagccaatgt ctacaatgaa
     1501 tgaagaagaa gtgtatacag atgaaataaa ttcagaagac atgagaggtt ttaaaaaatc
     1561 acaccatcat aatggatgta atactgataa taagtgtgag tgccatgatg attgcaatcc
     1621 atgcaaccca tgtaatccat gtaaacctaa cccatgcaat ccatgcaaac ctaatccatg
     1681 tgatgacaat tgtggatgcc atgacaattg taaatgtgat tgtgaaccat gtgaaatgga
     1741 ttcagatgaa tgttttgaaa acaaatgtgg accagaatgc tgtaatccta tatctccaag
     1801 aaacttctct gtatcaaatg cagtgccatt tgcaatagag gctaatagaa tatttgatac
     1861 tatgcaattc caaacattta cagatgcaac aggaccaaat ggagagccat taacttttga
     1921 aacagaagta gtagaagtat ttggttcagt tccaagtgca ggtcaagcaa gtgtaactat
     1981 agaaaaaata tgcttaagta atgatggaat cgttatagac acaggaatga caactttaga
     2041 agatttcgat ttagacccat taggagatat agtaggaaga aactgtgaaa caacttttga
     2101 atttgcagtt tgtggagaaa gaaactctga gtgctgtaga caaggaaaag gcaaatcagt
     2161 agcttataaa caaagaggat taactgtagc agttcgtaat ttagtactag agctaagagg
     2221 tagatgtgga tgtacagagt tcgttgcatt agctttccca gcagttagag caggaggtgg
     2281 atgtaagaga agagttgatt atgtagaatt tacttttaac acactttcag caccaatatg
     2341 cttgccagct gacggaagag ctgttacttt aagacaagaa tatcaaacta acttaactgt
     2401 agattgtata ggaaaatcta tattaaaatt agaatgcaac gaatgttgtg aacctttcta
     2461 tgaattaatt ataccaaatg atatagattt agtactttgc ttacaagaaa cagttagcac
     2521 attaataagt gaacaaatag tagttttagc atcaccaaat ccaatccaac caagacttgt
     2581 tgatactttc tctaaagtat gtgatttttc gcaatgtgga cctaatcatg gaagtggaaa
     2641 gccaagttgc cacagataga taggtagata tatagacagt ttaaattata aataaaaatc
     2701 atctaaggaa ggctaccaaa gtgtataatt tacattggta gccttcacgt ttttataact
     2761 agcgtataga ttttagtgat ttaaaaagca ctatagtatg taatagacaa gtaaagattg
     2821 atactatcgt cattgtgtat atgtatgcat aaatattaag tcctggaatt ggaagaaata
     2881 catataaagc tattagttga attaacatgc cagtaatagt atttatactt gaagctaatt
     2941 cttttcttat tgaatgtaaa atgccagata gagtttgatt tagggacata aatagtggaa
     3001 ctaaaaacat agcttttaga tattctcctg caagtgtatt tttaaatact aatatacata
     3061 aatcttttcc aaagaaataa aaaaatattg atgatagtat gcctacaaat aaagtaagaa
     3121 gtatggagta tttgattttt ttaattacac tcttacgctt tcttagagcc atttcttgtg
     3181 aaatagttgg tattaaattt acaataagcg ctgaaccaac agtaaaaggt aaaaatataa
     3241 aaggcattac catcccactg acaataccat acatacttaa agattgttga taagtcattc
     3301 ctgataatgc taatcttgat ggtatcatca tagaacttat agattgcaaa agtatgtttg
     3361 acattctatt acaagttatg ggaatagaca tttttaatgt ttccatagaa gaattataaa
     3421 aatcttttat gcttataata tacctattgt caaatgatga atctcgccat aagcaaataa
     3481 gcataaatat aatattactc aactcaccaa tcgatatacc taatagagct atataacaat
     3541 tcatagattt attatttatg tacataacaa gtaaaaaaac aaataatatt ttaccaattt
     3601 gttcaatgat ttgtccaata gcagggattt ttacattctt tatgccatag taatatcctc
     3661 ttagtacgtt tgaaagagtt attataacta tagcaggaca aacagctagt ataaatagat
     3721 ttaatttagc atcttttaaa aatttaagtg ataaaaaaga actattgaaa gatgcaacta
     3781 tagatataat tagtgctacg aaaaatgata tatataaagt tgatataaat aaagcattag
     3841 tattgtgtct atcgtttaaa gcttttctct ttgcaactag acaactaagt gcagtaggta
     3901 tacctgttgt tgtaacagct aagcaaatca tcaaaaagtt aaaaatcata tgatagatac
     3961 ctaaacctgt ttcacctagt gctcttgata agaatatttt atataaaaaa ccaaatattc
     4021 ttacaataaa attggatata aataaaataa ctgttgatag tactaaataa ggtactttca
     4081 aaaaaatcac ctccttatct tttaaatata ttcacaagga ggtgtaaaaa tttataattt
     4141 tataaattct ggtttcaatt tattaaattt agatacatat ttaaatccca tattttttag
     4201 taatgaatat atgtagtcaa actcacatgc aacttgactt atatgatgtg aatctgaacc
     4261 tgtggttatg atttcccctc tcaagtcttt gtacatttga agtatatact tagatggggt
     4321 taggtctgga agattatatc tataacaaga ggtatttatc tcgatacctt tgccatcata
     4381 aatagcttgt ttaagagttt cttcaattat atcagaaaat agtctatcat ctaaaatagt
     4441 gtcataaggt gcataccttt taatcaaatc taaatgacct aagacagagt aatctttata
     4501 attcttaaca atattatata aataaagata gtaattttca taaacttcat gttgagtttt
     4561 atttttaaaa taattaccaa ggtataagtc cattgtatct atagcatgga tagaacatat
     4621 tataaagtcc aatggatatt gatgtgcttc ctttttgtat gtatccatta attgagtttg
     4681 aactccaaat tcaattcctt tttttattga tattttatct ttatatttgc tttgaagagt
     4741 ctctaaactc ttaaaataat cttcgtagac aattgaaaaa gaatcatcag catatacatc
     4801 atagtcaaca tggtctgtga aacaaatttc ctccaaaccg agttcaatag atttttttac
     4861 catttcttcc attgtagatt ttccgtctgt agagaaactt gagtgcatgt gatagtcgta
     4921 aaacataaat atttcccctt tttataaaaa ttttggtatt atatatacat actaaataat
     4981 atcaaatata cataaaagca tcaataaaat ataaatacaa tgagcggtga taaatttgga
     5041 aaaatacaga atagatttaa atgagaaaaa atcaactaaa tatatacaga tatttaatca
     5101 tataaaagaa ttgataataa ataaagagtt agtagaacat gaaaaattgc ctccaataag
     5161 aaaactatca aattttttac aagtaaataa cactactata gttaaggtat acgagctttt
     5221 agaaaatgaa ggatatgtat ataaaattat agggagtggt acttttgtat caagtttaaa
     5281 ttcttataaa agcggtatag ataataggaa aaataagatt attgtgcaaa gagaagattt
     5341 aattcatttt gacaatggga atccatctaa tgatatgttt cctatcgatt catttaaaaa
     5401 tgctgtaaat atggcattat ctaaagatgg tagttcaata tttgaatatg atgaaggtct
     5461 tggctctgaa gaattaagag aaaaaatggt agagtatcta agtgatggaa atataaaaac
     5521 tactaaggaa aatattcaaa taatatcagg tgctcaacaa ggtatagata tagtttgtaa
     5581 aggacttata agttattcag atgttgtttt tatggaagaa ccttcttaca atggagcaat
     5641 tgaagtgttt aaaagtagag gtgcaaaaat aatatctata cctatgttag atgatggaat
     5701 tgatataggt attttaaaat taaagttgga aaaaataaaa ccaagactta tatacattat
     5761 gcctaatttt caaaatccaa ctggaatatc ttactcaaca tataaaaaaa agaaattgat
     5821 agaattggca gaagaacatg atttttatat aatagaagat gattttataa gtgattttgt
     5881 ttttgaatca aaggacaata gaacaattag aagttatgat gataaaaata gagtagtata
     5941 tataaaaagc ttttcaaaaa tacttatgcc aggtcttagg ataggaattg tagaaatgcc
     6001 aaatgagttg ttaaaaaaag ttttatgggc taaatattca tcagatatat caacaccagg
     6061 tttaattcaa aaatcaatgt attactatat gaataatttt gattggaaga attatttgag
     6121 tcatatagag aagatatata ctgataagta taaattggca aaaaacttga ttaatgacaa
     6181 attgagtggt aaattaaaag ttagaaaatc ttgtggagga ataaactttt ttttagaatt
     6241 accaagagga tatacatctc aagcatttac aaattttatg ttaaacaaag gagtgtcaat
     6301 gcttccaggg acctattttt ttgacaatct agtggatgat agattcttta ggataaatat
     6361 agctcgtcca agcatggaag aactagaaaa gggaatatct ataataagtg ataatataga
     6421 tgagtttttt tgtgaatata aaaacaagtt agatataaaa agcaataaat tattttatta
     6481 aataaataga ggttttttag gaggaaaaaa tgtttgacaa gaagaaatta gataggatta
     6541 atgaattagc aaagaaaaat aaagagggaa tcctttctgc tgatgaaatc aaagaaagag
     6601 aaattttaag gaaagaatat ttagaaaact ttagagccca ctttagatca agactagata
     6661 gtgtcaaagt agtttcacca gaagaatatg agcaatacat gaaaaacaat aaaaattaga
     6721 acttagtagg aggaaaatat gaacttaaag tacgaattta agaatgcttg ggattttgaa
     6781 agaaaagaaa atactattga acaaattatg caatattcta gcaattatat ggagttttta
     6841 agtaagtcaa agactgaaag actttcagta aaagaaatta taaaattggc taaagaaaat
     6901 aactatattt caatagatga agctatggaa aaaggaagca taagttgtgg tgataaaatt
     6961 tatgttataa ataaagagaa ggcagtagca ctttttgtaa taggtaaaaa ttatatagaa
     7021 aaaggtatga agattatagg aagccatata gattcgccaa gattagattt aaaaccaaat
     7081 ccactgtatc aagagagtaa tctaggtttt tttaagactc attattatgg tggaataaaa
     7141 aaataccaat ggacggctat tccacttgct ttacatggta tagttatatt aaatgatgga
     7201 acaaaagtgg acatatctat tggggaagaa gatagtgacc cagtattttg tgtaactgat
     7261 ttattgattc atttggctgg agaccaaatg caaaagaaac tatcagaagg aattagtgga
     7321 gaagctttaa atatattaat tgggaatatg cctttagaag atgaagaaaa agaacctata
     7381 acagcaaata tattaaagat tttaaatgag aaatacaata tagttgaaga agatttatta
     7441 agtgctgaaa ttgaagtagt tccagctggt aaggctagag atttagggct tgataggtct
     7501 atggtgttgg gatatgggca tgatgatagg gtatgttcat atgctgctgt aaaagctata
     7561 ctagaaactg aacaaccaga atttacttca gttgcattat gtgtagataa agaagaaatt
     7621 ggttctaaag ggaatacagg tatgcactca aagttttttg aaaatactgt ggctgaactg
     7681 attgctttag aaggagatta ttgtgatatt aaagtaagaa gagctttagc aaatagtaag
     7741 gtattatcag ctgatgtttc tgctggatat gacccaaatt ttggagaagc atatgaaaag
     7801 agaaattcgg catatatggg aaatggagta gttttaacta agtatacagg ttcaagagga
     7861 aagagtggat gtaatgatgc taatgctgaa tttatgtcag aagtaaagag aatttttaat
     7921 aaaggaaatg tagtatggca aactgctgaa cttggaaaag tagaccaagg aggaggcgga
     7981 actatagccc atatattggc aaatcaaggt gcagaggtta tagactgtgg tgttggagtt
     8041 ttaaatatgc atgcaccaca tgagattgtc tctaaagtag acatatatga aatgtataag
     8101 ggatataagg cattttttaa tataaattta taagtaaaaa tataaatgta ataagtgaat
     8161 aaaggctgtt gtataaacaa tttaatctgt tgtacaacag tcttattttt atgtaaaaat
     8221 agcaatttgt cacactagag aaaataaaag aaaatactgt caaattaaaa attatttaaa
     8281 aaaaaattga caacgatttc atcttgttat aatatgtaat aaagaacttg ttatataatg
     8341 ttataaaaat gttacatagg gaggtgagca aaatggatga aaagagacaa aaaaaagaaa
     8401 tggatttaga agtaaattcc tttgttcctt tatatcaaca actatatgac aacataaaaa
     8461 aacaaatagc atctggcata tataaaccag gagataaact tccatctgaa ggagacttat
     8521 gtaaagaatt taatataagt cgtataactg tgagaaatgc acttaatgaa cttgtaaaag
     8581 aagatatatt atgtaaaaaa cgtggtaaag ggacttatgt aacaatacca gagagaatag
     8641 aagcaacatg tgcaggaaat agttttacta attcttgtca tcgtattaat gcaaaaccaa
     8701 gtaccaaaat aatctctgtt ttaataaaaa aagcagataa gcaagtagct gaagctttaa
     8761 gtattgaact agaagagaag gtaatatgta ttaaaagact tagattaata gatgaagtac
     8821 ctgttatttt tgaagttgac tattttagaa tagattacat gtttttatta aaagaagaat
     8881 tagaagataa atctttaatg gaggttatat ctaataatat aagcacatta cctaaacgag
     8941 tagaaaatat atttgaagta aaacattcaa acaaagaata ttctgaccat cttaaatgtg
     9001 caagtaatat gccactattg aaagtaagac aatcggtgta cacagaaaat aatgatgtct
     9061 tatattacaa tgagcaattt ataagaagtg ataagtacaa atatgcagta tcagcagaaa
     9121 tataatctat aatttagtag gaggatgagt atgaaaaaga taatattagc atctcatgga
     9181 gacttttcaa aagggctttt aaatgcagta aaaatgatag taggagattt agcagattgt
     9241 gtcagttcgt atggtttgta ccctggacaa agtgcttcag attttgcttt agaattagaa
     9301 aaaacaatat cagaagataa agaatgtgaa tatataattt tatctgactt atatggagca
     9361 agtgtatgta cagcaatgtt aagattaact aatctacata atgttaggtt attttctgga
     9421 atgaatttga atatggtatt agaacttctt acaaggtttt ctgatgaatt aacagttgaa
     9481 gatatagacc aattagtgga agaatctaga agaggaatac aaagcgtgac attacaaatt
     9541 aaagaagaag aagaggtatt ttagtttatg aaaaaaaagg gaatattatc agtatttaaa
     9601 agtaaaaaac caattatagc aatgatacac ttaaaaggag atactccaga agatatcttt
     9661 gaaagagcta aaaaagagat tacaatcttt gaagaaaatg gtgtagatgg aataatgtta
     9721 gaaaattact atggtaatta ttatgattta gaaagaatac ttgaatatgt atcaaaagct
     9781 aatttatcaa taccatatgg agtaaactgt ttaaatgttg atactatggg atttgaactg
     9841 gcaacaaaat ataatgctag ttatatacag gttgattctg tagtaggtca tgttaaacct
     9901 agagatgaag ctactttaga agagtttttt aaattacaac gttcaaaatg cccagcgtat
     9961 ttaattggag gcgtacgttt taaatatcag ccagtattat cagaaaatga tgttgaagaa
    10021 gatttaaaaa taggaatgac aagatgtgat gctatagcag ttacagaaaa tgctacaggt
    10081 caagaaacat caatggaaaa aatagaactg tttcgtaaaa atttaggaga cttcccattg
    10141 gttatagctg ctggagttac attggaaaat gcaaaaaaac agcttaaatt aggagatatg
    10201 gcaataatag gaagttactt taaagataat tacaaagatt ttggggatgt tagtgtagaa
    10261 cacgtaaaaa cattcatgga tgaaatcaaa aaaattaggg aggaattata atgattaaat
    10321 tagtaagagt agaccacaga cttatacatg gacaagtagc atttacatgg acaaagtttt
    10381 taagtacaga ctgtatattg atagcaagtg atgacttatt aaaagatgaa ttgagaatgg
    10441 caggacttaa aatggctaaa ccatctaatg ttaagttagt aatgaaaagc atagcagatt
    10501 ctataaaagc acttaactca ggtgttactg ataaatacaa cttattgata ctttgtgaat
    10561 ctgtagaaga tgtttataga cttgctaaag aggtaaaagc tattaaatct ataaaccttg
    10621 gtggaacaaa atcagatgat aatcgtgaaa atatatctaa agcagtgcat gtatcaaaag
    10681 atgatataaa gatgattaaa gagttagatt cagaaggcgt aaatgtattt gtacaattag
    10741 ttcctgatga tgatgctaca aatgtaatga aattaatata attttagggg gatgaaaatg
    10801 gaatttacac aagttatttt gataactcta attgcattct ttgcatatat gcatagtttt
    10861 gtaggttcta caatgcataa tagacctata gttgttgcac cacttgttgg tttagcacta
    10921 ggtaatcttc atacaggtat agtaataggt tcaacattag aattagtatt tatgggtgca
    10981 tttcctgttg gggcaagtaa tcctcctgat tttgtgtctg gttctattat tgccacagcg
    11041 tttgtaatat taacgggtca agatgtatca gcagcagttg tacttgcagt tcctatagca
    11101 actctagtat tgcttataga taacttctta atgacagtag tacttacatg gggagctcat
    11161 attgctgacc gttatgcaga agaggggaat attgaaggcg tagaacgtgt gcaattatta
    11221 tttggtatag gaaataaatt aatattagca ataatagtag gtataggatt ttcattaggt
    11281 gttccagtaa tagaaaaaat actttcattt ataccatcat atgtaactca tggaatggat
    11341 gttgcagcag gcgtgattcc agcaataggt tttgccatgt tagctagaat gatgcttaat
    11401 aagaaaacag tagcattttt attattagga tttatattgg ttgcttacct aaacataaca
    11461 gtaacaggag ttgcattgtt tggattagca atagctttaa tatatgttaa ctttgtagga
    11521 gagaaggagg taatagtaga tgacaacgag ttctaagaag ttagagacaa tttcaccaga
    11581 tagtaaaata acgaggaaag acttttggaa atgttttaga aggtcattga cattagattc
    11641 atcttggaac tacgaacgta tgcaaaatat agcatatgca tacatgatgg caccaataat
    11701 ccgtagatta tataaagatg ataaagaaaa gaaatctaag gctttaaaaa gacatttaga
    11761 atttatgtca gttactcctc atataagtac tcttttggta ggtatatctg gagctatgga
    11821 agaagaaaat gcaaagaata aagaatttga tgctaatagt ataaatgctg ttaaatcaag
    11881 tttaatgggt ccagtttctg ggataggaga ttaatttttc tggggaacat taaagcttat
    11941 agcagctggg gttggtatag ctttagcttc tcagggtaac ataatgggac cgattttatt
    12001 ccttttaata ataaatgttc ctcattttat tatacgctat atatgtctag ataaaggatt
    12061 taagtatgga actcaattct ttaaagatgt gagtggttca agtatagttt caaaagttat
    12121 ggaagcagct tcaatgttag ggcttatggt aattggaggt atgactgcat ctaatgtaat
    12181 gttaaaactt agtgttaatg taggtagtgg agaatgggca gagcctattc aaacttactt
    12241 agaccaaata atgccatgta tgcttccagc tatgatattc ggtattatgt attggttatt
    12301 aggaaaaaag gtaaagacaa ctacgatatt aatttcagtt atgattattt gtatagtatt
    12361 ggctgctatt ggagtagtat aaataaaaaa atcccaagtt catttgaacg agggattttt
    12421 tgactaaatt aaataaaaaa gaaagatggt tcataataat ttataagcat ttatgtgata
    12481 gttataatta gcttattaat tagttataaa ctcaaaatta agcactactt tattaaagtt
    12541 attatattat aatttcgaaa tactcacata atctgtttag aaatttatct tctaatgggg
    12601 aaaaagtttt ctttttagag taaatgaaga aaaactttct agtaaaatca acatccttta
    12661 ttttataaca ttttatttta cctgcattga gataatcgat agctgaaatg tacgatataa
    12721 aggacactcc aagacctaat ctaaccattt ccttaatagc ctcatttgat tctacatgag
    12781 cgattacatt taatttactt actggaaagt tatttttact aagtgtgttt agtattaagt
    12841 ttcttgtacc tgacccttct tttctcataa taaaatttag ataagccaat tctcctatat
    12901 cgatacaatt atttttatta tcaattttaa aatctgaagg agtgataaga acaagttcat
    12961 catctaataa gtctaggtac tctatttgag gattattgat tttagaacca actaatccaa
    13021 agcttattcg ttcatttaaa atttctgata tagcatattg agaatcataa tggcttatgg
    13081 aaaattttac atctggataa ctcatagaaa aactcttcat aaaatcaggt aaaatataag
    13141 tttcaggtat ggaactacat gctatatcta taataccttc tatttttcct gaatattctt
    13201 ttatatcata aattgcacgt ttgcaattat tcaatatata tatagcatgt tcgtaaagaa
    13261 tttcaccaga ttttgtgagt gtaataactt tgttgcttct atttataaga acagtttcta
    13321 gctctctctc taaattctgt atgtgtgcgc ttacagtagg ttgagttaaa aaaagttctc
    13381 ttgcagcctt tgaaaaactc tgatgttttg caacagcaac aaatacttca agttgtttaa
    13441 aatccattat ttaatcctcc ttaaattatc taaaactatt ataacatatt tgataaatta
    13501 atagtcaaag gtgattttta aaatagaaaa cctttaaata ttcatggagt ttttgattgt
    13561 ttaggaataa tttggtataa tgttgtaagg tacttacaat aaaaatatcg taaattgaat
    13621 tttcaaaatt caactttaaa gtgtttttta ttacagaaaa attacaatta gcttcttaat
    13681 cttattaaat tcaaataaat attatataat tattttataa tataataaat ataattattg
    13741 aaaaaagaga taactgtaaa ttgagatata gataaatact ataaaattta agaaaggagg
    13801 attgatttat gaagttaaaa aggtcattag tttgtgtaac tattttaggt attatacttg
    13861 ttggatgtca taaagaaaat actaaagaga aaaatcaagt agcaagtaaa gcaacacagc
    13921 aaaaaactat gacaaaagtt caaaatgatg ttaatgaaat tatgaataaa gattataagt
    13981 acattataaa gaatatgggt ataccttata atacatttta ttatataaaa cctaaagttt
    14041 taaaagaatc aaatacaatg caagatataa atacatcaag ttatatgaca ttagtttatc
    14101 caaaatatac tggaaatgat gaactagatg gaagtgcttt atatgttgat attaacgaaa
    14161 ataaagttgt caatgttgaa accaattctt tctcatcaca aggtatatca gtcattgatg
    14221 ctgaatctag tatagtgatt gaaaaaagtg accatgaaaa atcggctgta tcactagaaa
    14281 attttagaca tattgacctg ggagagtatg ttggtgttga agattccaga ataaatgaaa
    14341 ttgtaggaga tgcgaattat gatttaacag catataatca tgaaggcagt aaagtagtta
    14401 agagttatag attgaaagaa gataacaaaa tattaaaaaa agaggtactt acaataagta
    14461 ttgtggataa caaaataaaa tctataaaaa ctattgaaag tgataaaatt gtaaaaataa
    14521 taaaaggaac tttattagaa taaaatattc taaagcatat gatgctgttt caagatagaa
    14581 ggaaatttct agctgggaac agttttttta ttagatttat aaaatttgaa ctgcacttat
    14641 agataactat tagttataat taaatataag ataa
//
LOCUS       DAEMIK010000111        14500 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEMIK010000111 DAEMIK010000000
VERSION     DAEMIK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.111"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      complement(427..510)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(743..1585)
                     /locus_tag="KPK87_002741"
     CDS             complement(743..1585)
                     /locus_tag="KPK87_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2538875.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1837..3621)
                     /gene="pepF"
                     /locus_tag="KPK87_002742"
     CDS             complement(1837..3621)
                     /gene="pepF"
                     /locus_tag="KPK87_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG2538876.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3687..4352)
                     /locus_tag="KPK87_002743"
     CDS             complement(3687..4352)
                     /locus_tag="KPK87_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2538877.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4503..4664)
                     /locus_tag="KPK87_002744"
     CDS             complement(4503..4664)
                     /locus_tag="KPK87_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG2538878.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4742..5251)
                     /locus_tag="KPK87_002745"
     CDS             complement(4742..5251)
                     /locus_tag="KPK87_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG2538879.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5457..5675
                     /locus_tag="KPK87_002746"
     CDS             5457..5675
                     /locus_tag="KPK87_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2538880.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5693..7639
                     /gene="feoB"
                     /locus_tag="KPK87_002747"
     CDS             5693..7639
                     /gene="feoB"
                     /locus_tag="KPK87_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2538881.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILLRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7799..8854)
                     /locus_tag="KPK87_002748"
                     /pseudo
     CDS             complement(7799..8854)
                     /locus_tag="KPK87_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
     gene            complement(8902..9702)
                     /locus_tag="KPK87_002749"
     CDS             complement(8902..9702)
                     /locus_tag="KPK87_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2538882.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9695..10486)
                     /locus_tag="KPK87_002750"
     CDS             complement(9695..10486)
                     /locus_tag="KPK87_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2538883.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTMMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10526..10948)
                     /locus_tag="KPK87_002751"
     CDS             complement(10526..10948)
                     /locus_tag="KPK87_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG2538884.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10964..11443)
                     /locus_tag="KPK87_002752"
     CDS             complement(10964..11443)
                     /locus_tag="KPK87_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2538885.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11575..14331)
                     /locus_tag="KPK87_002753"
     CDS             complement(11575..14331)
                     /locus_tag="KPK87_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2538886.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4704 a         2488 c         1649 g         5659 t
ORIGIN      
        1 tgaatattgt attaaacatt aagattcatt attaaatact aattaaagtc tccgattgtt
       61 ggagaatgga gcttaaagtc attttcacgt ataaatttat tatttttcta gaaacttgtc
      121 tattggctag tttgagtatt gatttatatg atttatatgg ttataatata aaataaattg
      181 tattattttg gtaaatatat tgttacaaaa ggtttatatt ctgcatctca atgtttataa
      241 aaactttatt ttcttattat aatactatta ttcttttaaa aaaatttccc ttttccaaca
      301 aatggctact ttaaaagcca aaattttacc tatcaataaa aatcttaacg actaatactc
      361 ttattttcaa attttgcaac atttttgtta attttaaaac aaaaaaagac tatgcaatat
      421 aaaaattata ttcatagccg gttgcaccac taactccata tatctccaga tgcccaataa
      481 taagatatac ttctcagttt ctaaaatatt tttattaaaa atcttttata tattttcata
      541 aatttctttt ttattctaac attgtaatat tattttgtca atatttccat attcttagag
      601 ataagatact aaaataccta ttttatctaa agatatttat atttctacat tcaataatag
      661 acaataccct gcaagttaaa gcatcatttt tatgagctta tctcacaggg tattatatgt
      721 ttactttata ttataactct tgttattgat taactttaat aaatcctttt tctgaaacaa
      781 gtgcttgtcc ttcatcactt aatatataat ctattaaagc tttacctttt tctgacaaac
      841 catcttcttt attaactaat ataaatggtc ttgatattgg atatttatta gctattacat
      901 ttgcatcagt tgcttcaaca tcatttattt ttaaactatt tactttctca tcaacatatc
      961 caaatgatat atatcctata gcattttcat ttccttcaac tgtagttttt atatttcctg
     1021 aaccatcact tatttgtgca tctttagtta gttcttctga atcaaaccct acaatttctt
     1081 ggaatccatc tctagttcct gaaccttctt ctcttgatac tattactatt ggtgcatcat
     1141 ttcctcctac ttctttccag ttagtaatct ttcctgtata tataccttta acttggtcca
     1201 ttgtcagatt ttttactgag tttgcagtat tagttatcaa tgctatacca tctattgcta
     1261 ttttagtttc ctttaatcca gcactttttt cttcatcttt taaatctctt gaactcattc
     1321 ctatttcaga tgttccttct atagcatttt ttatacctcc agaagaacca atctgttgta
     1381 cttctacact aacaccttga tttttttctt gaaacttttc tccgatagtt tccataagag
     1441 gacctattga agttgaacct gaaacagaaa ctttgcttga atctcctgaa tcacctgaac
     1501 ctcctgagtt agaactacat cctactgcta aagctccaac taacattgta ctagctaaaa
     1561 gaacgcctat tttcttttta aacattttac ttacccccgc ttatatttta attttttgtt
     1621 gtcatttttc tatattttaa taatacagat tctttgttaa atgaatatta gagcagggtt
     1681 aagtaaatgt aaatattgtt aaatacatgt aaaaacttaa ttttatatga tttattctta
     1741 attagattaa ctttttaaag ttactttagg cagatataaa cataatacaa ctcatattat
     1801 cagaagtctt cattgaagct atcaacctat cacatcctat aattcttttt ctagtttatt
     1861 aactaattca gcaaatacat ttaaggcttc ctctatagat tcttttttcg tcatatctac
     1921 accagcagat tttaattgtt ctagtgggta ttctgaccct ccacttttta agaagtttat
     1981 atatctatct actgcagtac ttccttctga aagtatctgt tgacttaaag cacttgcagc
     2041 agaaaaccct gtggcatact tatatacata gaagtttgaa taaaaatgag gtattctcgc
     2101 ccattctatt cctatttgtt tatctactat tgcagatttt ccataatact tttcatttaa
     2161 tttgtagtat atattagtaa attcatcagc agtaagtggt tctccactct ctaccctttg
     2221 atgtacaatt ttttcaaatt cagcaaacat agtttgtcta tgaactgtag ttctaaattg
     2281 ttctaaataa taatttaata agtatatagt ttcatcctta gacttagaat tttctaataa
     2341 ataatttata agaagtaatt catttaatgt agatgcaact tctgcaacga atatcttata
     2401 gtccgaatat aaaaatggtt gacttgttct tgaataatga ctgtgaacag agtgccctaa
     2461 ttcatgaatt aaagtgaata gagaattaag gtcattttta tagctcatta atatataagg
     2521 atgtgaatca taacttcccc aagaataagc tcctcctttt ttaccttcat tatcatatac
     2581 atcaatccac ccttcatcaa aagcactttt tatgaccttc aagtattctt ctcctaaagg
     2641 tttaagtgcc tctaatatga tatcctgtgc tttatcatat ggtatatcca tgtcaaattt
     2701 atctgtcaaa ggaacataca aatcatacat gtgtatttca ttcaatccta aaaatttctt
     2761 tttaagctca acatatttat ttagattagg aatactttta tctatagctg atataagatt
     2821 attgtaaaca tctaaactta catcatctga aaataaagat gcttctattg ctgaattata
     2881 ctttctagtt tttgcgtaaa atatctctga ttttattcca ccataaagcg ttgaggcaaa
     2941 tgtattttta tatttctcat atgttgaaaa ttcaccttca aaagcgtctt ttctaactct
     3001 actattctta ctttttataa ataaagagta gtttgaatga gaaagcttaa ccttattccc
     3061 ctcctcatct tctatctcag gaaattccat atctgcatat gctaacatat cgtatacatt
     3121 ttctggaatt gtcgataatt ctgatgtagc tgcaagtatt tcttcctctc gttcactcaa
     3181 agtatgtggt ttatctctca atatatcctt tatatatttt gtataaaagg atagcttctc
     3241 atcttttaag tattcactta acttattctc ttctatcgcc agtatttctg gaactatata
     3301 agaagtagcc acacctaaat cagtagataa catttctgtc ttagtggctc ttccttgatt
     3361 atcattaatt ctagtatctt catgttgttt catatgagta tatacatata aattttgaac
     3421 aatcctagat gcattttcag agatatttaa tactttatat aagttctcct tactctcaga
     3481 aagcttccct ttatattctt ttacttcttg gataagattt tttactttac ttatgtcttt
     3541 ttcaatttcc tctttactgg agtacatctt atctatcttc catttgtact tttcttctat
     3601 tgtacttcta tcagttccca tatcatacac ctctatttta atttgtttta tattttattc
     3661 actttacaaa atatattgta tatattttag tccaaataat ctacatttat tactccttct
     3721 atcatattta tttctttcat aacgtcgtgt aagtgtattc tcttagcata agatacaaaa
     3781 aaattaacct tccagtattc ttcattacga cattctattt ccatatttct tacgataagt
     3841 tgattttttt caaatttatc atataaaaaa ggtatcgagt ccaaattgct taattttaat
     3901 atcaatacat tcttttcttg gctaaataaa aacgtattga ttatcctaag cacaaataat
     3961 gctatcatta caaataatcc acctagaatt gccaattcat aataaccata tccaactgct
     4021 ataccaatac atgcagttgc ccacatccca gcagctgtag taagcccttt tactccacct
     4081 ttatttttta aaattgctcc tgcacctaaa aaaccaattc cagataatac ttgagcaggc
     4141 attcttgcgg ggtcagcatt tgtatacaaa ttataatcaa agaataattg aatcgaagtt
     4201 attgaaacaa tacaagaacc tattgcaatt agtatatgtg tcctaaatcc tgcaaactgt
     4261 cttatttttt ctctctctaa gcctattatt cctcctatta ttagtgctaa tagtaacctt
     4321 attgctatct ccttacttcc tatatcaatc ataatataaa accctcctga cttaattaca
     4381 acttatgtcc tacagataat ttacacatca gaaatactat tttatatctt aatattatta
     4441 ttaatatatg taaaatatag ccatctgtta atttttgata caaaaaaacc cacaactgtg
     4501 ggctatttgc ttaggtacat gtatttattt attaatatat ctattttctt acttgattct
     4561 actaactctt cttgaaatat ttctggatat tctatgtatg tatttatctc ttccctaact
     4621 gcttcaatct cattttttaa cgcttctaat tcattgttcg ccataataat caccactatt
     4681 acaaatttgt atatttttat atacatttta tcaaacaata ttatattttg tcaatattat
     4741 gtcaataaaa cttactataa aaatgcttta atttaggtat tgtatacctg gatactaagc
     4801 ctacaaacat accagttact aatgatgcca ttaacataaa tggtaagtac cctatagcaa
     4861 gtatattttc tatgattaaa cttgcaacta ttaattgacc tatattatgc cctattgcac
     4921 ctataatgct tatccctatt agtgataaat cttttatttt actcgctaaa tacataaata
     4981 aaagactcag ataagctcct cctatactaa atagtagata tgataaaggg cctgcaaata
     5041 ttgatgacaa aattaccctg atagtaacaa ttataacagt atctttgaca cctaacaaca
     5101 ttaatgaaat taaagttata atattgctca aacccaattt tgcccctgga aatataacta
     5161 taagaggatt tggtatatat gtctctaata catataaagc taaactatac cctatcataa
     5221 gacctaaaaa tgtcattttt ctagttttca ttaagttaac tcctccaaat tttatctatc
     5281 atcttacatt ataatacaaa atatataaaa acacctaatt tagactaaaa tatttttaat
     5341 ccatttagaa taaatattag ttatttcaat gtgcaattac ctctataaat cttgactttt
     5401 tgtatcatac ttgttacaat tttaatgata atgaatttca ttaggaggtg ttaataatga
     5461 ctgtttacga tttaaaaata ggagaaaaag gatttattaa tagtatagat ggagatatta
     5521 aattatctaa aagattgctt gcacttggat gtataaatag tactgaaata gaagttaaaa
     5581 aggttgcccc acttggagac ccaattataa ttagatttag aggctttgac cttgcgatta
     5641 gaaaatctga tgctaaaaat atttctttac aataatagag gaggagctac ccgtgattaa
     5701 tgtagcatta ttaggaaacc caaatgtcgg taaaactact gtttttaatc tacttacagg
     5761 ttcaaatcaa tatgtaggta actggcctgg tgttactata gaaaaaaaag aaggatttct
     5821 tggaaaagag ataaaggttg tcgatttacc tggtatatat gcaatggata ctttctcaaa
     5881 tgaagaaaag gtttctaaat catatttaga aaatgaagat gttgatgtaa tagtaaatgt
     5941 agttgatgct tctaatttat caagaaacct ttacttaaca actcaactta tgcaatttaa
     6001 caagcctata gttatattgc ttaatatgtt agacattgca gaatctaaag gagttaatat
     6061 agatgccaaa aaattaagtg aagaacttgg agtaattgtt gttccaatta tagcaaaaaa
     6121 gaaaaatggt gttgatagga tagaagaaga gattaaggga gctacgcaaa aagcttttat
     6181 atatcagaaa aactttggaa gtgaaacaga aacatataaa aaattagaat ccattttatt
     6241 gagatgtaca aaaactacat atacacaaaa gaaatctatt agtgataaga tagacaatat
     6301 agtcttgaat cctatattag catatccaat atttattggt gtattgttcc tcttatttaa
     6361 atttaccttt gactgggtcg gaggaccact acaagaggga tttgctagtc ttatagaggc
     6421 ttatatttct gcacctgtta gtgatatgtt atctaactct agcccatggt ttaaatcact
     6481 tatagttgat ggaataattg gaggtgttgg tggtacttta ccattcttcc ctttaatatt
     6541 tacattattc tttggtatat cgttgtttga agatagtgga tacatgtcaa gaacagcatt
     6601 tttaatggat aaagttatga gaaaggttgg tctatctggt aaagctttta tacctatagt
     6661 tatgggtatg ggatgttctt ctccagctat aatggcaact agaacattgg aaagcgaaaa
     6721 agatagaaga gttacagctc ttattgcacc acttatgact tgtggagcaa agctacctat
     6781 atatgcacta tttgtagcta tattctttcc acataatgct gctttagtaa ctacatcact
     6841 gtatttggtg ggtatagtta tggcaattgt agttgcatta ttcttaaata aaacagcctt
     6901 taaaacagag gctgaaccat ttatattaga attaccagaa tatagaatcc ctactataga
     6961 tgcacttatt aaaaatactt ggaataaatc aaaaggtttc ttgataagag ttgttactgt
     7021 catgtttgca atgtcagtcg taatttgggg actttcttca ttcaatgttt ttggatatac
     7081 agaagatata aatgtaagct tcttggctac tctaggtcat attatatctc ctatattcaa
     7141 acctcttggt tttgatgatt ggagaacttc agttgctatt ttaagtggtc ttggagcaaa
     7201 agaaatcgta gtaaactctt taaatatact atatggtaat ctaactgttg tcttaccaac
     7261 aatatttaat ggagttactg catatacatt cttgatattt actgctttat atactccatg
     7321 tatagctgca cttgcaacta tgaaaaaaga atatggcaat aaaatgatgt ttacatcatt
     7381 tgcatatcaa tttatactag catggataat ggcttttata gttaaaagta taggtggagt
     7441 actatttatg ggtaattcaa taatggaagc tttgataggt ggaataatta tagtccttgc
     7501 actctttatt ctgtttaata aattcaagtc tacaaaaaac ggaaatgtct gtagtggatg
     7561 ttctatttgt tctggatgtc caagtgctaa ttcttgctca agtaaggaag acagcaataa
     7621 aaaaaataat gcacactaat atataaatat tttagtttaa aataagataa aagtgggtgt
     7681 ctcaaaatta actttttagt tcatggggca ctctttttta tagaaaatca aatatatttt
     7741 catcatttca acagtaaaaa agaggcttat ctctattttg agacaacctc tttttttgtt
     7801 acttattcag tttcttgcta cctaacttca tgtggaacct agtatctttt ggaacacttg
     7861 ggtctacacc tcttaatgta ggtatttccc cacatattag ttgtataggt actataaaat
     7921 taaatgtttg atagtaagga ttatctgtga tatcaatctt taaattttta tcattttctg
     7981 taacatctct acctatagca aatacatttt ccgtccaacc agaaagtaca tcaatcatct
     8041 tagttactct aggttctttt catgtatcta gtataaatat agtagaatca ctatttatag
     8101 cattatatat accatgtata aactcttcaa actcataccc agttacaggt attctcattg
     8161 tttccaataa ttttaatgct gcttctaatg tatctccata tatatcagag tgacctatta
     8221 ttcttatttc tttagaatta actaactttt ctttgtttct ttcaatccat tgtttagaga
     8281 gcttatacac tgcttcaaat ctattaattg catctaatat cttatttatt tcctcattgt
     8341 acttttcact agatattata cctttttctc tagctatttg aagacctaat aacattaagt
     8401 ttaactttgt acaataatat ccttttgttt tagctccaga tttttcttca ccacaattta
     8461 ctgttaatat ataatcagaa atctcatcta ttaaagcatt cttacaacct gccatagatg
     8521 ctattttaca gcccttatct tcagctaact tcatagcgtt gtatgtagaa taactactcc
     8581 caccttgaga tacacctact actagggtat tttcattatc aaacttgaat gtatcttcag
     8641 ttatcataaa tggatacatt ttcacaacat ctatatctaa taaattttgt aaatatggtt
     8701 gcacttgcac tcctgagtga tagctagtac ctgaacctgt tattataatc ttttttagat
     8761 ttgttctctt aacttcatta aataagctat ctgcattaga tataatttct ctcattctaa
     8821 ctggtgtttc taacatataa tcttgtattg tcattttata caccttcctt atctatatat
     8881 tcaataaatt ataattaatt tctataacaa acctatagca tgtaaagcga ttcctgttac
     8941 tatagtgcct atagttaatt ttgttacact tacctttctc tttaacatgt agtatattac
     9001 aaatgcaaag gttaaaggta acatattagg tattatttta tctagtatat cttgtaaaat
     9061 tacttctgca ccatttaagt tgaatttcag aggagtagtt atactcaaca tagttgcaac
     9121 cattcctcct acaacgaata aaccaacagt tgtagccata gacattattt tctccataag
     9181 tccctctctt tgtattcttt caagagaatt tacccctact tgataaccat acttaagtcc
     9241 aaatattcta agggcaaatg ctggtatatt gtataatagt aagaataata gtggtccaaa
     9301 gatattacct tcttttgcaa gtgatactcc tactcctgct gctataatcc taaatgttcc
     9361 ccagaataat gaatcaccaa ttcctgcaag aggtcccata agagctgttt taacagcact
     9421 aattgaactt tcatcaaatt ctggattatt agcattctct tcttccatag ctgctgttat
     9481 acctaatata gcaggaacta ctactgtcgt tgtattaaaa atctccaagt gcctttctat
     9541 tgcttttgct tgattttctt tcttattgta tagcttcttt attgcaggta acattgaata
     9601 acagtaacct aagttttgca ttctttcgta attaaatgca ccttgaagag caaatgaacg
     9661 ccaaaacata cttcttaatt ctttttttgt taatttattc aaagtcaatc tctccttccg
     9721 ttaaagtatt gattgctaat tgaggatttt gattattttc tttgttattg atataaaagt
     9781 ttagtacaaa tgctacacat actccaaata aagctatagc agttatatct aatccaccat
     9841 aagatgctaa caaaaaccct ataaagaaaa atacagacat cttttttgaa agtaacatat
     9901 ccataagcaa tgcaaaacct actgctggca acaatgttcc tgcaacaccc agtccgtcta
     9961 atataacttt aggtattgca tttatcaatg ctgtaacttt atcagcacca atcataattg
    10021 ctaaaaaggt tggaactgat gtacttaaaa agaataatat tactggcaac cacatcatca
    10081 tggtaacttt cttaaaatca gcctgtttag catagaaaac tgccttttga gaaaagtacg
    10141 aatttattat tctaactaat actccaagag attgtgctaa gactgctact ggtactgcta
    10201 ctgctaaagc aacttctgca ccattacctg ataatatagc aaaagctgta cctaatacac
    10261 ctcctgttac tacatctgga ggagtagctg cacctattcc tgcaattccc atccaaatta
    10321 attcaagctg tgcaccaatc ataattcctg attttatgtc tcctaaaact aatcctacta
    10381 acatacctgt aactattggc ctatcaagca tagattgacc aaatatacgc ccatctagta
    10441 taccaactcc tgctataata gctaacatag tagcttgtac taacatattg aacctccatt
    10501 ttaacttagt ttaatttaca ttaaattaca ttacattttt gattttattt tgattttcta
    10561 ataactcatt tacataaaca attccatttt ttgcttcctc taatcctgat ttaactatta
    10621 gttttgtatc ctcctcaact aataaaagta tagataaaag cattggtaaa tttactcctg
    10681 tcaataattc aaatttgaac ccttgtaaaa gtaagtttgt acatatgttt gccacacttc
    10741 caccaaaaac atctgttaag acaacaaatt cttcactatc tttattattt gataatatac
    10801 ttctaatttc tttctcagca tcgtctgctg atttatccgt tgtcatacaa aaatactcaa
    10861 tattagttaa tgaaccaatt attaatttac ttgtgtcaac aagctctctg gacaattctc
    10921 catgtgttgc aattagaaac tttttcattc tctttctcct aaattatttt attaaatctg
    10981 atagttgttt ctttttgtct ctaggtaaca ttctaaattc tatatcaatc cctaaatcac
    11041 ctatttgttt aaagttattt atatcatcat catcaacagc aactaaatct gtaagtttct
    11101 tcttcccttc ctcatatctt aatcctccta catttatttc ttttaatact cctcctgaat
    11161 ttttagctac aactaaagca tctttggtat tttttactaa cactaataca ttgcttttct
    11221 ttgaagaagc aaatttttct actatttctc cagattcctc tacccccctt atatataaag
    11281 ttacattgct aggtttcgct aagttcagtg acatagcttt catttggtca ttttttgctt
    11341 catcatttgc aactagtatt acatttatac ctagtgatgt tgcccatcca tatgcaacct
    11401 gaccatgtat caatctgtca tcaattctca tcaaagatac cattttcccc aaatcctcca
    11461 taatacatta aaatttttat tgtttatact ctcatttatc tataaaagca aaaagtatgc
    11521 caaatactgg catacttttt taaatttcat tcacttattt tgtaatcttg tattttactg
    11581 tggtagacca tagataatat tataaatata tcctatctct gaaattggta tttgcacact
    11641 ataactcttt tctagcacac taaatgcatt ttttattagt tttacttcac tatgatggtt
    11701 tttgattaac aaatccatat cagtatactc ttcaatttca gtttgtctaa ctaatctttc
    11761 aatcatacaa cttaagtgaa catataaaga aatcttttta tcatttgaaa gttttttctt
    11821 ttgaatattt tctatagcat taattccttc ttctattctc tctattatct tttttgaatc
    11881 taaaatagtt agactatcta ttactctaat taatgaaaaa ttctttacaa tagaatcatt
    11941 tatgttcttt attttttcat tttctactac tctgctaaat attctaaaaa catcatcttc
    12001 tccttttcct gatattatat cctctaataa tataaaatct accccattca catttggatt
    12061 ggcagttcct actatagcca tcacatcata catttcaaaa actggttctt tatctcccat
    12121 agaaacaaga ctatcataat cataaggtat tattttgaca tcaactattc caattaaact
    12181 ttcggaaagt agatgttgga tttttatagc agtacctatc cctgtaaaac aagatgatat
    12241 tatagcatat tctttatttt tttgaggata atacaattgt atattaggtt tagtttcttc
    12301 atccataatt tctgcaatct cttcaatgtt tttaccttgc attaataaat ttcctacttc
    12361 tagtgcagaa atagtggata tttggtctat aaataacatt gggctattta tcttattctt
    12421 taaatcttca tatataagat taagagagcc catatcaaat aatacaatta aaccattctt
    12481 tatcctatct ccctctaggt atctagacat ttgttttgat acatcagcaa tagtagcatc
    12541 tataggcata tcaatagctg taaatatatt tttacccaac atccgattac atacattcgc
    12601 catactactg gcagtagcat atccatgtgc tataatagtc gcattaactt gattaaattt
    12661 cattcttata attgttgatt taataaatat aatcattatg attgtatcta cattattaag
    12721 atacatatcc aatctattcg ttattaatgt tgatatttta cttattatag agtattcttt
    12781 gtaactctct ttttttaaat atattagtaa tttattttct aaagctttat actcatcaga
    12841 caattgataa gattcgtcct tcaaataaag ataagacgaa aatgccaata cattattccc
    12901 atcatatttt atattgttat taacttctaa atttctaaat atatcctgca ttatcgatat
    12961 tataagctcc ataagtacgt tattattttc atcattattt gaaaagacta aattgtccat
    13021 tagtctaatc attagattag aactcttttt actaaatttt tcctttgtta tttcattttt
    13081 ttctagtcta tcaaagtaat ctaaacattt tatgaaactt tgatagatta tatttctttt
    13141 atttcttcca tttgacattt caatactaaa ttgtttagga gaaaagataa tgcttttgct
    13201 tttaccactt tggaattgaa atatatctac attttctata taaagatttc taggaaggtc
    13261 ttgaagagta acaaaaatat ttccatttct tctttgattt tttgcataag ctcgtccaca
    13321 tgcatatttt attacatttt tacattcacc tatattcccc ttgaatttta tctttaaaat
    13381 cgcatttaat gtaatttgag ttatctctat aggcaaacca agtaccttac tttcttcata
    13441 aataaaatgt tctacaatct ttcttttttc aatagtccct ctttcttcta tattgggaac
    13501 ataaatattt atgggtattc ttcttaaaaa ggtttgtaaa aattcagata gactttctgt
    13561 tgtagcaaat attaatctta ccctagcttt tctaattact ccattctctc caatcctaga
    13621 aaacgtacct gtatccataa atgtaaataa cttttcctga ccttcactat ttaatctatg
    13681 tacttcatct aaaaataaaa caccaccatc tgattgttct attagacctg ctctagattc
    13741 atttgctcca gtatatgctc cttttacatg cccaaataat agactagaca ataactccaa
    13801 attattataa tactgcgcac agtttaacac attgtatgga gaattttttt ctaatacatt
    13861 actgcttaca ctatatttgt atattatctt ggctaaaaaa gtttttccta ctccactttc
    13921 acctgtcagc attattgtaa gtccgccatt tggataaagt acagcggttt ttatttgttc
    13981 gattacttct tttaagctat tatcatatcc tatgatacta ttaaacacag tctcttgctt
    14041 taataattta cactcatttt taagttcatc taaattttca taaatacttt tttttgtagg
    14101 tcctactaca tcatttattt tatttttagg taaaaaaata acaggtctac tgtttatctt
    14161 tataagatta tcagatttta ctaaatcatt taataatcta cttgattgag ttctacttaa
    14221 attaaacttt tttactatat cttctgttat tatgccactt tcttttttaa gttcatcaaa
    14281 gttgttaaat aatacacatt catttacaaa acttaaaatt tcatccctca tgttcaacct
    14341 ccaatacaaa tatcaataat actataatat tagtatatca tttatatatt tatgaaaaag
    14401 ataattatat tcctatcttg attctctttt gtaaggttgt aaaatcaaac tttttataag
    14461 tataaaatct agatttttta acataatctc aaggttctta
//
LOCUS       DAEMIK010000112        14396 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEMIK010000112 DAEMIK010000000
VERSION     DAEMIK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.112"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            66..3164
                     /locus_tag="KPK87_002754"
     CDS             66..3164
                     /locus_tag="KPK87_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2538887.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3287..4573
                     /locus_tag="KPK87_002755"
     CDS             3287..4573
                     /locus_tag="KPK87_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2538888.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(4926..6032)
                     /locus_tag="KPK87_002756"
     CDS             complement(4926..6032)
                     /locus_tag="KPK87_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2538889.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6067..7608)
                     /locus_tag="KPK87_002757"
     CDS             complement(6067..7608)
                     /locus_tag="KPK87_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2538890.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(7970..8716)
                     /locus_tag="KPK87_002758"
     CDS             complement(7970..8716)
                     /locus_tag="KPK87_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2538891.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIKKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFTESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(8928..10238)
                     /locus_tag="KPK87_002759"
     CDS             complement(8928..10238)
                     /locus_tag="KPK87_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2538892.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKKEYLTFLEKHDKTL"
     gene            complement(10251..10721)
                     /locus_tag="KPK87_002760"
     CDS             complement(10251..10721)
                     /locus_tag="KPK87_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538893.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10725..11576)
                     /locus_tag="KPK87_002761"
     CDS             complement(10725..11576)
                     /locus_tag="KPK87_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG2538894.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            12500..13411
                     /locus_tag="KPK87_002762"
     CDS             12500..13411
                     /locus_tag="KPK87_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG2538895.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            13586..14083
                     /locus_tag="KPK87_002763"
     CDS             13586..14083
                     /locus_tag="KPK87_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG2538896.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
BASE COUNT         5471 a         1806 c         2230 g         4889 t
ORIGIN      
        1 tatagcaaaa ttttaaaagc tttaaaaatt caatctggaa aatgaataaa aggagatata
       61 tataaatgga tgtaataaat ttaatagatg agttggatga aattgtatat gtttcttctt
      121 tagatacata tgaactttta tatattaatg aaattgggaa aaaggcaatg ggacttaatg
      181 atatatccca tttaaaatgt tataaagcat tacaggggaa agattctccc tgcgaatttt
      241 gtacaaactc tattttaaaa gaagatgaat attacatatg ggaaaatacg aatactcgaa
      301 caaatagaca ttttatatta aaggacaagt taattaaatg ggagggcaaa catgcacgat
      361 tagaaatagc tcttgatatt acagaaaagg aagatataag taagtcaata gcagaaaaat
      421 tagagataaa ggagttatta gttggatgta ttaaacatct tataacagaa tctaattttt
      481 ctgatgctgt aaatcttgta ttaaccaata ttggtaaatt tcataaagct gatagagcat
      541 atgtttttga agtatcagaa gatagaacaa aagtaactaa tacatatgaa tggtgcaatg
      601 aaggtgttga tgaacaaaaa caggcactaa caaatcttga tattaatgaa ttaaagaatt
      661 ggtttttatt attcgaacaa aaaggttttg taataattga tgatatagaa aatataaaag
      721 aagaacagcc aatagaatat gaaattttaa aacctcaaaa aattgaaagt ttaatgacat
      781 cttcactaaa acaagatgat aaagtagtag ggtttattgg agttgataat ccacataaag
      841 ctgttggaga tacctctctt ttgacatccc tatcttattt tttattaact gagatgaaaa
      901 aaagaaaagt agaaaataaa ttaaattata tgagctacta tgacttcttg actggtgcat
      961 ataatagaaa taaatatatt aagtatatag ataattttca aaaaggaaaa gttaaatcca
     1021 taggagttgt atttgtagat ataaatgggt taaaagatat taatgataac tttggacatg
     1081 ctaatggcga ttctgctatt gtgtcagcat gtgtaataat aaaaaaatac tttcattctt
     1141 ataatgttta ccgtattgga ggagatgagt ttgttgtatt atgtgaagat atatcaaaac
     1201 aactatttga agaattagtg agttggttaa gtgaagaatt taaggatgag atgatatata
     1261 gtgtagcagt gggcagtatt tggagtgacg ataatattga tatacataag ctaattaaag
     1321 ttgctgatga gagaatgtac caagataaga agaggtatta taataagaac aaacttagaa
     1381 ataatgaaat gaaagtaatt agtgacttag ctgattcgac gaataatatt ttatttaaac
     1441 ataaagagtt agttgataag gtttattttg atgatttaat gataaaaggg tattctaaag
     1501 cttatgaaaa attagcttat aatacatatg atataatttt atctatttgc attgatacga
     1561 atttaattac tccaatttat caatcagaga gcaatatttt tagttttcct ataaaggaaa
     1621 atcttgaaaa aactttttca tacataaaaa aatatttaat tcatcctgat gatataatat
     1681 tatttaatga taaccacctt ctaaaatctc ttgatagtct taataatagc aagcaagacg
     1741 atttagaact aaattttgag tatagacgtt taggaataga taaaaagtac tattgggcat
     1801 taatttatat gttaaagatt agtagtaaaa ataagttagg tgagaaacaa acacatattt
     1861 tagttgctat aaaaaatata gattatctta tgcaactaca taaaaaccaa aaagatacat
     1921 taacaggact atacaattat gaaagttttt gtgaacaaat aagtttaatg cttaaaagat
     1981 atcctgacaa gcgttatgca attataatga tggatataga taagttcaag gtaattaatg
     2041 acatttatgg agttagagga ggagataaag ctttaatata tgttgcagag ttgattcaaa
     2101 aaaacattgg ttctaatggt atatgttgtc gtatgtatgc agatgtattt tgcatttttt
     2161 atgagagtta ctctgataga gatattcata aagtaattta taagattaaa aatagcttaa
     2221 atagaaaaaa atttgaatat atgttagtac catgttttgg ggtttataga attttggata
     2281 atcatattcc aattacaaat gcgtgtgaaa tggcaggata tgctcacaaa aaagtaaaga
     2341 aacaaagtat caacaactat atattttatg atgatactat aagaaatgat gcacttgaag
     2401 aaaaacagat ggaaagggaa atggaatacg cacttgaaaa tgggcaattc catgtatatt
     2461 tacaacctaa gtataatctt aatgcaaata aaattgttgg tgcagaagca ttggttagat
     2521 gggttcatcc agaaaaaggt ataatacctc caattaaatt tatttcacta tttgagaaga
     2581 atgggtttat tatcagatta gatatgtatg tatgggaaca agtatgttct ttgattagaa
     2641 aatggataga tttaggtgaa gagccagttc ctatctctgt aaatgtatca agattacatt
     2701 tgcataatcc acattttaaa gaaattgttt taagtttaat aaataaatat agtataccaa
     2761 aatctttttt agaattagag cttacagaaa gtttatttat taaaaatata aaattattta
     2821 ctaagataat agccgacctt agaatggatg gtattgtttt aaatatggat gactttggtt
     2881 cagcatattc ttcattaaat atgttaaaaa atattaatat tgatacatta aagatagatt
     2941 gtggtttctt taatgaggaa ggaatcacag acagagaaaa aatagtttta aagcacacta
     3001 tagccatggc aaaagattta gatatggatg tagtggcaga aggtgtagag acaaaagaac
     3061 aagctgaatt tttagtgagt ttagattgtg tggtaattca aggttattat tattgtaagc
     3121 ctattccaag tgctgatttt gagaagttac tttatcaaaa ataagctaat tattccattc
     3181 taaatgtagg taggatggag tagttcaaaa taattaaata gaagtagaaa atagtgagga
     3241 tataattttt aaaaagtata atatttatgg attaggagcg aagtttatga tagtgggtat
     3301 tataggtcct tcagactctg ggtttaagat aaaggatgat ttaaaacaga tagacagtga
     3361 tttaaagact aaaatatatg taagagaaaa agtagtagat acaatagaag ttatttctaa
     3421 atgtgaagac gaatgtgatg ctattatatt tacaggatgt gcagtttatg aatttataaa
     3481 atctaagtat gagataagta agccacattc atttgtaccg agaagtggga cgagtataat
     3541 gaaggcattt tgggcaataa aaagtgcaaa tataaaatta gataaattca gtatagatgt
     3601 agttgataga tatgttgtgg aagatgcact taaagaattt gatattaaag caacgagtgt
     3661 attttgtaat ccattttctt tggaagtagg agagtctgaa cttgtagaat ggcatataaa
     3721 attatttgaa gaaaataaga cggatataat gttaactggt tttggtgcag tatataatga
     3781 attaaaagaa agaggctatc ctgtatttcg tttacaagct actatacaat taatcaaaga
     3841 aagttatgac aaggtaaaga gtgaatatgc tctaagtaag gcaagatttt ctcaaattgc
     3901 tgttgaaata ttgagtttaa tagattataa agagaagata gataactatt attctgacat
     3961 gataaaaaaa tcagatatgg ataagcttgt agtaaactat gtaagaagta ttcaaggttc
     4021 cttattttta cttggaagaa atgattatgt tgtatttgtt cataaagggg tagctgataa
     4081 tgagtataat tatgataagc tattcaatct aaagagagag ataaaagaca tgggcttttc
     4141 tcttagtata ggaattggta ctggagtaac tgcgtatcag gctgaaaata atgcacataa
     4201 agcattaaga cactccatag attcaaaaga aattggtata tatttggtag atgaagatga
     4261 aaacataaga ggacctttag cttccgaaaa tgaattaaat tattctttga tgctatctga
     4321 tgaaaaggta atcgaaatat ctaaaaagac tggaatgagt tgtgagtcag ttgcaaaaat
     4381 tattgctata aatgagaata gaaaaagtaa gatttatgat tcaaaagagt tagcagagta
     4441 tctaaatgta agtgaaagaa gtgcaagacg tatattaaat aaaatagtaa atgcaggttt
     4501 aggtcgaata tgtgcaaaag agactagtat tggtggagga agaccaaaaa atattactga
     4561 aatactgttt taagatattt tataaaactg tataaataat tatattatat aaaattctac
     4621 aacaaattac tatataaagt attaaagctt attatgaaaa aaattagaac aaattattat
     4681 ataaaatact agagcttgtt attaaaaata ctagaataaa ttattaatat aaaatactag
     4741 agcttgttat taaaaatgct agaacaaatg attatgtaaa atactagagc ttgttattaa
     4801 aaatgctaga acaaattatt atataaaata ctaaaaccta tgtaaaacaa ctttaatata
     4861 taacttataa agttgaagat acataggttt ttatttttat aatttaaaga gttatattat
     4921 gatatttata atttaatcat atgataaact attttggcag tatcatataa ttcgtctata
     4981 tttacatatt cattaactgt gtgtacatca tacataccta gacttaatat agcacaatta
     5041 tatccaagat tagctaatat atttgcgtca cttccaccac caattaccat tggatttgga
     5101 acaacaccaa cttgtcttat tgcttcttca cttaacttga aaacgtgact atctctacta
     5161 agttcaaaac tagggtaaga catattatgt tcaaaagtat aagtagtatt aaatttagat
     5221 gcagcatcct tacaacattt ttccatatga ttaagctcat actctaaagt ttcaggtata
     5281 tgagacctaa tttcagcagt caatgtaacc ttatcagtta ctatgtttgt agctccacca
     5341 ccttctattt tacctatatt tgaagtagtc aatgaatcta ttctgcctat gtgcatgttg
     5401 cttattgcat gagaggctac taatatagca tttaaccctt tttcaggttc tataccagca
     5461 tgtgctttct ttccatgaaa agagattttt atagattgtt gagcaggagc tttgtatgct
     5521 atcgaaccaa tagctccacc agaatctaaa atcaccatat ctttacaagg caagttgtca
     5581 ggattaaagt ttttgacacc atgcatacca gcttcctcac agattgtaaa acaaagataa
     5641 atatctctat gtggtatttt ttctgtaata acatgttcta aagcttcaag tatagcagct
     5701 attccagctt tatcatctgc accaagtgta gtagttccat cacttctaac tacattacca
     5761 tctagtattg gttttacacc taaacaaggt tgaacttggt ccatgtgtgc acataaacaa
     5821 agtggtctac ttccttcttc accttttata taggcaatga cattacctgt attaccacca
     5881 tatttttcac ctgcatcatc tataatggca tctatatttc tttcttttaa gtagtttacc
     5941 aaccacttag acatttctaa ttcttgatta gatgggctat caattctaac catatccata
     6001 aaattattta taagtctatt tttatctaac ataaattatt tctcctttta gaaagattta
     6061 aataaattac atgctatatg aaggaagtat tccaggtcct agtggaatat taaataccat
     6121 aaacaatatt aatagtatag tccacactat taagaatgat atagagtatg gtatcatatt
     6181 agcaattaca gtacccatac cagcatcttt atcgtatttt cttgtgaatg ctagtataat
     6241 agggaagtac ggaaataatg ggctaagtgg atttgttata caatcaccta tacgataagc
     6301 catctgagta agtgctggat tgtatccaac catcataaac attggtacaa atataggtgc
     6361 aagaatagcc cactttgcag atgcactacc tataaataaa tttataaaac atgataatat
     6421 aataaatcct atcaacaata aaggtccatt tattcctgct gattttaaaa attctccgcc
     6481 ctttacagct aatatcagac ctaaattact atttgtgaat aattgcaaaa attgtgaagc
     6541 agcaaatgct agtattatat atccacccat atcactcatt gcactagcca ttatagagac
     6601 tacatcctta tccttcttta ttttacctat agtttttccg tatacaacac caggtatgaa
     6661 aaatgctagt gctattatag gtaccatacc tttcataaga ggagcattgt ttgatagtaa
     6721 atctcctgtt tctgggtcag ctaagaatgg ttttttacca attacactaa gagctactat
     6781 aatagccaca tatattaata tacttatacc agctttttta agccctttct tttctacatc
     6841 actaagactt ccatcaacat ctagttctgc atttccttca tatttaccaa atctaggagc
     6901 tacaattttt tctgttaccc atgttgccaa aagtattaga acaaaagttg atgctatcat
     6961 aaaaaataag ttcattgttg cattggcttg aaatccagaa tcaatcattt gagctgctgg
     7021 aactgtaaaa cttgcagcaa gtatatcatt gacacttatc ataatgtttg cggcaaatcc
     7081 tagacagact gctgcatatc cagaaaataa acctataagt ggatgtctac caacaccaag
     7141 atatacaagt gcagctaatg gtggaaataa tattgtacct gcatctgaag ctatattggc
     7201 aagcatacct atagtcatta caacaagagt tactcttgtt tcaggaatct tgcttactga
     7261 tgctttgatt gttgctgtca taagtcctgt tttgtcacat acaccagcac ctatcatagt
     7321 tactagaact agacccaaag gagcgaaccc ttggaaattg ctgactatgt ttcctaaaag
     7381 aatttgaagc tgttctacag tcaataaatt tacagcggtt atagtttctc cagtaccagg
     7441 atgaactact gatatcccca tagaaccaac tagactagat agtataagta aaagtacaca
     7501 taaccctaaa aatattgtaa ctgggtctgg tagtttatta ccaacagttt ctattctgtt
     7561 taatgtcctg tcaaaaaagt ttttcttctt aactacgtta tcattcataa tatcctccct
     7621 aatgtttaaa aatataatta aaaaataagt attgtggtta atcttacaat ttaaattctg
     7681 tttgtcgatt taagacgtgt ccacaatatg tcctaaaaaa agtataaaat aaactttttc
     7741 ctgtgtcaat gcagttttct gaattttctg gttgagtagt gttagttaaa tatgctttaa
     7801 gtagattaat gacgaatttt atctaatgta tttaaataac atatgcactt taaaattaat
     7861 tttaaaacaa aattttatga aaagctagtg gaagattaag ttattaaaat aagtagcgta
     7921 gtaaagtgtt agatttttta attaatcaaa gaaatttact ttgtattttc tacaaaaatc
     7981 tgtgaatatc ttttgaatta tgtagaggat ataaatttat tttatttaca tagattagat
     8041 tttgttctaa tttactgtat tcaaatggtt ttccatgtcc aggataaatc attttaggtt
     8101 ttgtattgat aattgttttc caagattgaa taaattctag tttgctttct gtaaatatgg
     8161 taattttgtg agtacttgga aatccattca ttgcagaatc tccacaaaat aaaacaccat
     8221 catcaattag tagtgaaatc gaatcagcag tatgtcctgg tgtatctata atttttccat
     8281 ttagttcttt tttaatttct ttacgattct tgtcagacac aagaatacac cttttttcaa
     8341 attcttgttt aagtggagaa aaactatgct caccttttcc aaatagtttc ataatttggc
     8401 aaaagagaaa ggcagtacgg ctggtacaac caccaataaa agagttttga cctttatata
     8461 gaatttctaa agctttatca tgcataacaa tctttgtatt aggagattcg tctagaatct
     8521 gatttagaaa accaacatgg tcatcatgag catgagttag aaatatgtat cgaatatctt
     8581 tgatttttat gttgaattta gaaattcgtt ttttgaaatg tttaaaacca ttttcatatc
     8641 cagtatcaat caagacaaag ccatcttgaa tagggtacaa ccatgtattt ataattcgat
     8701 ttcctgcatt aaacattttt tcaccacctt gaattagaat agaaatttat attaattaga
     8761 aagaatatag catagttcta ctgatatttc aacgtagctc taaaagtttt aaggaggata
     8821 atatatttta gtttaatcta aaaactaaaa gaaaagaagt atactgttac aaaaacaaag
     8881 agtggtataa cttttacagt agataccacc ctattataaa aatagactta taaagtttta
     8941 tcgtgttttt ctaagaatgt tagatattct tttttagtca taactggagt aaaggtatct
     9001 aatatatctt tagcatcctt agcattatca aaaagaaggt ctacaactgt aagtgccatt
     9061 gctttagcag gaattatgta agctaaatct tcatcaacta tttcaaaatc tcttgtatga
     9121 agagctcctt ttacaccacc aatcataggg tgaagagtag gcattatatg agacacatct
     9181 ccaaaatcaa aagagcctgt aaaatctcca ccatcaataa cttttccttt tagacctaaa
     9241 tcttcaagat tgtttttaaa taaattatca agacttttgt atcgtaatat tggaagatat
     9301 ccaggaattt cagttatctc aacatcagct ccaactgcca ttccaccagc catcaaagcc
     9361 ttatttattc tttcattagc atcaatcata ccatttatag ttctagctct aacatatgat
     9421 tccatatgaa catcagcagg aacaacattt actatatctc caccttttgt gatgataggg
     9481 tggaatctaa ctctttcaga ctctttaaaa gtttctctaa gtgcattcac gttgtttata
     9541 gcaagcattg ctgcatttaa agcatttatt ccatcatgtg gagcagaacc agcatgagat
     9601 tctttaccta taaatttaac cttttttcca acaaatccat tactctcagg accaactaga
     9661 gccttatctt ctcctaaatc agaagcatga aacatcatag atatgtctat atcatcaaaa
     9721 taacctcttt ttattaattc ttgttttcca ccaaaataag ttatttttcc ttcttttttt
     9781 aattgctctc tgtattccaa ctcaataaac tcttcagcag gagtagccat aaaagatact
     9841 ttaccatcta aatgttctaa aacgccactt tttactaaac caacagcagc acctaacatt
     9901 ccagctattt gaatattatg tccacaagta tgagaagcac ctatttcatt agcatcttta
     9961 tgttctttac atgatatacc atccaattct ccaagtatag atatatgagg acctttcttg
    10021 ctagaatttg catctgcttt gcatcctgta actgctatat ttttttctac agataaattt
    10081 aattcttgct caaaaaagtt tgaaacagct tctgtagttt taaattcttt atatccatat
    10141 tctggatttt tgtatattcc ttgacctatt tttaatattt tttctctatt ttcatctata
    10201 gtttttatta ctagagattt taattgttct ttattcataa taaattcctc ctatatttta
    10261 ccttgaatac ttaaaactat atgagctata ctcgcacatg caaagaatat agttgtaaat
    10321 acaataagtg aaattataac tattttccat gacatttctt ttaaagcttc tattttgttt
    10381 ccaacagaaa ttcctgcaaa agctagtagt ggagtagtta tagccataaa gttaatatta
    10441 ttagtgttag ttaagaatac ttcagatgtt ggactatatg gcatactaag taaaaggccg
    10501 attatagttg caaatccaaa agctggaaag attgatttag gaaataaatc ttttataatc
    10561 atagcagcca tagcagctaa tattagcatc accataccag gaagagctgt aactatagat
    10621 gtaccaacgc taattctttg tcctataaga attataactc caataacaga cataaccaat
    10681 aattgatatg aacttttctt taaatttaaa ttagcaaaca tttattagca ctctccttct
    10741 gtagatttaa tttctttagt ttttttattt ctcgaaaatt ttggttctaa aatcttatac
    10801 ataaaattag taaatggtaa ggatacaaaa actaagaata taattcctgt tatactagac
    10861 atcatattgc ttgttgcagc ataggcaagt attgtatctg ccatttcagg tggtaccatg
    10921 gtagataatg aaccagcagc tgctgtcatc atacttccac taccaacacc acaggccatt
    10981 cccaaagcat atgggtgaag accactataa atagatattg aacctagtac actaaagtat
    11041 atagtaccaa gaactgttcc cattaaatat gtaccaagta caccactacc ttctggtgag
    11101 tttatcccat atttttctga tataactcct aaagaaggtt cacgacttat acttgcagta
    11161 gctccaacag cttctctttt cattccaaac attaatgcaa gaggtaatgc aacgattggt
    11221 gcaagtatat gaccaaattc ttgagcgata aatgctggtc cagcctgaat tattttgtca
    11281 atgtttggtc caactgtagt accatatttt ataccaagca ttactagtgc aattccaaca
    11341 acttcaccag caatatttat ttctttttct ccaaccactt ttttaagtgc tttaattttt
    11401 ttacctaata aatctggtgt tattataagt ccaattataa ctgcataaag catagggaac
    11461 agtactatag aggcttttcc aatagctata gttctttgcc ctataaattc acatattact
    11521 accaagatta agcaagttaa aaatacttga ataaaatgtt ttttattaat ttccatgatt
    11581 tcctccccaa aagaataaat attttactta tctgaaatga gaaaatacgt tttcttaagt
    11641 ataatacgtt ttcctaaata aaatgaaaaa actatttcaa ataagtttca gaaatgtcaa
    11701 taacatgtcc ttaattaagg ataaaacaaa aatttaacat tgtcaacgaa aaatatctaa
    11761 aatattacat attttgaata tattgtaata tagctttatt atgaaaaaat tgtttaaata
    11821 ttgtaggaat aatagctaaa aagctttaaa attagtaata taaaattgaa aaataataat
    11881 atagagttga gaaatagtaa attgaaattt taaaataaat gagtggaagt agcttagtaa
    11941 agtatggaaa tgaatattac aatagaaaat aaataatatg taaagacttt aaattaattt
    12001 taagtattaa gtacaaatac taattttgat atagaaattg aataaagtaa atgataataa
    12061 gcaaatgaat taagatgata ataagtgaat tgatatagaa ataagtaaat taagtaagac
    12121 gataacaagt gagataataa taaatgaatt gacatagcaa taagtaaatt aagtaagacg
    12181 ataacaaatg agatgataat aagtaaattg atatagaaat aagtaaatta agtaagatga
    12241 taacaagtga ggtaataata agtgaattga tatagaaata agtaaattaa gtaagacgat
    12301 aacaaatgag atgataataa gtaaattgat atagtaataa ataaattaag atgataatat
    12361 ccaaagtgag aaagataata gaaagtaaat aaaatcatgt aagaataatt tagataagat
    12421 tctaagattt tatttattta aaaattagta agaataattt agattaaata aatttaataa
    12481 ttttagtagg tggtaatgaa tgaaaataaa tagattaaca gaaataatag taattctatt
    12541 aaataaaaaa ttagtaacag ctaaagagtt ggcagatagg tttgaagttt ctacaagaac
    12601 catatataga gatatagaaa ctctgtctat gtcgggagta ccagtatata tgacgaaagg
    12661 caaaggagga gggatatctt taatagaaga atattctata gacaaggcta tattgtcaaa
    12721 aaaagataaa gagagtttaa tagtggcact gaaaacatta caggcgacta agtatccaga
    12781 aataaattca gtagtaaata aaattggctc aatatttgga gagcagaatt tttcaaactg
    12841 gattgaaata gactttactg aatggggaag taattttaat gaagatgata aatttacaaa
    12901 aataaaagaa gctattttaa ggcgtaatac tataaatttt aattatgtaa atagtttgtc
    12961 aagccagaca aacagaactg tcgaaccact gaagcttatg tataagagta agacttggta
    13021 cttgtatgga ttttgtaaac taaaagatga ttttaggata tttagaatta gtaggataag
    13081 gaatttatct ataaaggatg aagtattttc aagaaagata atagaagaag tatgtttgaa
    13141 tgattctaaa gtaattaagg aaaatactat aacgttgaaa ttgaggttta aagaaaagat
    13201 gctttttaga gtatttgatg attttaataa ggatttaata actaaaaatg aagatgatac
    13261 atatgatgta attacagaat ttcctattgg tgaatggatt tatggatata ttttatcgtt
    13321 tggtgacaat gttgaagtac ttgaacctaa agatgttagg gataatgtaa taactagact
    13381 aagggagttg tctaaaattt attcattata atataatgct agattaaaga ttttattaaa
    13441 tatataatat cttgcattta gtatataata tctcactgag tttaattata ttaaaaatat
    13501 gacataaagg tgtcgtatat tataaattat aattgaatta gttaaaaata aaatatatta
    13561 ataaaaacaa agtgaggtat aaagtatgaa ttatgaaata gtagagataa atgagaaaat
    13621 agttatagga atatcgaagg agactacaaa taaagatgga caagcggtaa atgatatagg
    13681 tgaattgtgg aaaaaattta tgggcaaagg gatatataat gctataaagg gtaaaaagaa
    13741 tgacaagaca ataggtctat atacagatta ccagggtgat tttacaagtc cgtatagctt
    13801 tgttgcatgt tgtgaagtaa atagtaattc tgataaagag aagaatttag aagaattaaa
    13861 cactaataat actgttggag aaagtattat tagtaaagtt ataccagcag gtaagtatgc
    13921 aaagtttgtt atagtaggag gtcaaaaaga agtaggagat ttttggttta agttttggca
    13981 aatggatttt gataggactt atataagtga ttttgaagag tatcaatgta atacttttga
    14041 tacaaagaaa caagaagttc atatttacat aggaataaag tagatttgtt attactatat
    14101 tgaatgaagt attgagtata gtacatttat tatagatagt tttattattt ataactataa
    14161 gcagtaaatc tgtaaaagat ttactgcttt tttagtaatt aatattatca gtatcacttt
    14221 gaatcttcag acatattctt catttaatat tgtttattga taaatgatac ttgtaaatat
    14281 aatgatttgt tatttaattt aaaatatgaa aaaaagtatt ataaacacat tagaatactt
    14341 ctggatttat gagtatacaa tttgaaaaat ataaaaaata atgtataaag ctaaat
//
LOCUS       DAEMIK010000113        14345 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEMIK010000113 DAEMIK010000000
VERSION     DAEMIK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.113"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            147..860
                     /locus_tag="KPK87_002764"
     CDS             147..860
                     /locus_tag="KPK87_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG2538897.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            978..1847
                     /locus_tag="KPK87_002765"
     CDS             978..1847
                     /locus_tag="KPK87_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2538898.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2002..3147)
                     /locus_tag="KPK87_002766"
     CDS             complement(2002..3147)
                     /locus_tag="KPK87_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2538899.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(3480..4256)
                     /locus_tag="KPK87_002767"
     CDS             complement(3480..4256)
                     /locus_tag="KPK87_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG2538900.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            4838..5812
                     /locus_tag="KPK87_002768"
     CDS             4838..5812
                     /locus_tag="KPK87_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG2538901.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            5910..6215
                     /locus_tag="KPK87_002769"
     CDS             5910..6215
                     /locus_tag="KPK87_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2538902.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            6371..7531
                     /locus_tag="KPK87_002770"
     CDS             6371..7531
                     /locus_tag="KPK87_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2538903.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLIKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            7581..8483
                     /locus_tag="KPK87_002771"
     CDS             7581..8483
                     /locus_tag="KPK87_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG2538904.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            8563..9456
                     /locus_tag="KPK87_002772"
     CDS             8563..9456
                     /locus_tag="KPK87_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG2538905.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      9509..9690
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9918..11048
                     /locus_tag="KPK87_002773"
     CDS             9918..11048
                     /locus_tag="KPK87_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2538906.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            11120..12145
                     /locus_tag="KPK87_002774"
     CDS             11120..12145
                     /locus_tag="KPK87_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2538907.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            12148..13344
                     /locus_tag="KPK87_002775"
     CDS             12148..13344
                     /locus_tag="KPK87_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538908.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(13472..14197)
                     /locus_tag="KPK87_002776"
     CDS             complement(13472..14197)
                     /locus_tag="KPK87_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2538909.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRRY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         5416 a         1641 c         2493 g         4795 t
ORIGIN      
        1 taagtggggt taagcgttat aattaattat aaatattgaa taatctaaat ggataagata
       61 atggtattaa aaattatctt aaaaggtgaa ataatctgta tttaatgtag tacagttata
      121 tctttaaagc aaggtggtga aatgatataa ataagatatc taaaagaggg tttgatgata
      181 gtgataaaag atggttttca agtaaagaat taacaagact tataaaggct aaagaagaaa
      241 tagaatggtt attaaatagg gattacaaaa tagacccagt cgttactttt gttggtgata
      301 gatatcagtt ttctataaga caaagagatg cattaaaaag ggcagtctgt actgatgaaa
      361 aaaatattat aagacaatca aagaggcttt ctctagataa aatcaaagaa gataccgtaa
      421 atatagatgg ctttaatttg ataatatcca tagaggtagc attatctggt ggtactctag
      481 taataggaag tgatggcaat ataagagatt tagcaggtct taggggaacc tataagatta
      541 tagataaaac agaagaagct ttgaatctga taggtaaatt ttttaataaa tacaatgcac
      601 agaatcttaa attttattta gatgctccag tttctaattc aggaaattta aagtatagga
      661 tattagagca tgccaaaact tggggaattg aaactgaagt agaattagta aaaaatgcag
      721 atgttgtatt agaaaaatta gatagagtag ttagtagtga tgcagtcata gttgacaagt
      781 gtataagtta ttttaatgtg gctagaggta taattgaaga gtatattaaa gaatgtaata
      841 tcataaattt aaataaatag ataaatatag gtgagaatat ataagtatat gtagcaatat
      901 tattagtaat acaagtaaag tttgaaaata ggagaacgaa tacatatcga atttcataaa
      961 atgttgtaaa aagacatatg agaggatgga tatgtgtgaa attaactaaa ctaaatatta
     1021 aaaaatatag agcacctata tataaatatg ctttagcaaa tgtaaatttg aggtctgcta
     1081 aatctaccaa ttcaagtatc ataacagtaa tacctcaggg agctaaaatg gaagtattgg
     1141 atgaagaaga tgactggatt aaggtgatgt acaattctca agaaggatat gtgtataagg
     1201 atttggtatc agtaagcgaa tatgcttgga gtaacttaaa cttaagagaa gataaatcta
     1261 caacttccaa tataattaca gtaattcctg aaaaatctag ggttgaagtc cttcaagtag
     1321 atggagattg gagtaaggtt gtatatgatg ataaaatagg atatgttttt aattattttt
     1381 tatcaattga tgggaataaa ccaaatgaat tagattataa gtatttttat actgacatga
     1441 ttaaatttgt taacgaaaat aatattaaaa gtactagcga ttatctaatt gtaacagacc
     1501 ttagaaataa atatacttat atttttaaga aagataatgg aggctgggga caactttaca
     1561 agtggcaatg tactataggt aagcctgaga ctccaactat aacaggtata ttttatataa
     1621 gtggaagaaa accttctttt ggaacggatg agtattcagt taaatatgct actagaatta
     1681 aaggtggata ttactatcat tctgtgctat atgattcaac aggaagttat ataatagatg
     1741 gcagacttgg tgaagctctt agtcatggat gtataagatt aagtacggaa aatgcaaagt
     1801 ggatatatga taatatacca gatacaacaa cagttataat tcattaagaa aaattttgag
     1861 ttttttattg ggataatgat tcttaaatta ataggattat atattaactt ctcataaaga
     1921 gtactattta aaaagtgtaa taagagagtg ctattaacaa aacaaagata ttttgaggca
     1981 ctctcttata aatatacata attaagatat tttcataaac aattctataa tcttatcttt
     2041 atcaaagaaa acaggattag caccagcaca agcatctttc atagagtttt ctgcaagcaa
     2101 ttcaaaatta ggattatcaa cacctacatc ttttaaagat tttgggattc caacctcttc
     2161 agaaagagct tttatcttat ctattacaaa gtcaacacac tctttatctg atttattatt
     2221 tacatccatt ccaataactt cagctattgg tcggaatttg gcaggggctg atttagcatt
     2281 ttcctcctca actattggaa gtagcatggc attacatact ccatgaggta aatcatataa
     2341 acctcctaat tgatgagcca tagcatgtac atttccaaga cctgcattac taaaggcaat
     2401 tccatttaaa aaacaagcat aacacatttg ttctctagct tctatgtcat taccattttt
     2461 aacagctctt ggaaggtatt taaatatctg ctttatagca tataaggcag ttgaatctgt
     2521 aacatcataa gcaccattag ctactacagc ttctatagca tgagttagtg catccatacc
     2581 agttgctgct gttaaattag atggtttgct aatcattaat tctgggtcat ttactgtcat
     2641 tgtggctagt gaattggtat caaccattat catctttaca tgtctgtctt catcagttat
     2701 aacatagttt attgtaactt cagcagatgt accagcagta gtagttatag ctatgatagg
     2761 aagacatttt ttagaagttt tgttgatacc ttcatagtct ctaatatctc ctccattagt
     2821 ggctaatata gagatagctt taccacagtc ttgtggagaa ccacctccta tagttataac
     2881 gaagtcgcaa ttttctgatt ttaatatttt aagtccttta ttcacattag ttacagttgg
     2941 attttgttta acttcatcat aaacaacaaa gtctatatct atctgagata aaatatctgt
     3001 tacttttttt acagtaccat ttgaaactag aaatttatca ctaacaataa atgctttttt
     3061 agttcctaat gatttaattg aatctttagc ttcattaaga catcctttcc caagtaaatt
     3121 tattggtggt acataataaa tgctcataac taaaatctcc ttttattttc attgattttt
     3181 atgtaaaaat atatatctaa caattttcac taatttactc ataatatatc gtatcttaga
     3241 ttgagatagg gttgctgatt cttatataag aacttaattc gtaattacga ttatgattag
     3301 aatttaacat agtattttta tcatgtcaat aaaaatataa aaaatattat aaaaaaaaca
     3361 aagttactgt agttaaatca gcaactttgt ttttatagta taaatcaaat aaatacatta
     3421 gtttaaaaca aaatttttaa ttattatgtt ttgatatctt ccatataaag aatattaatc
     3481 taatttatta caatgtcttc attacgttct tccatcattg attgaatatt tttctgagca
     3541 tttaagagta atttttttat cagttctatt ttttcgtcat tagctctaaa taaaggcagt
     3601 gttacactaa cagcagctct tatacatcca ttttttctta ttggtacggc aagacatttt
     3661 aggtgttcca ttgattcctc acactctgaa gctatttgtg ttctttgaac ttcaagaagc
     3721 tggtcgtata aaacatcaaa atctgtaatt gatttttctg taaaaccttt tagaccatct
     3781 ggatataagc ttattagttt ttccttgtca aaatcacata aaagagcttt tccaagacct
     3841 gtacaattag caggtatttt ttttccaaca taagaaataa gtctaatcgg ttctggagaa
     3901 tctacttttc caatataaag aacttcatta ttgtcaagta taccaagctg acaagtttca
     3961 gaacaggaat taactatatt agtcatttcg tcaattatat agttaaaaat agttcttttt
     4021 tcaaaaaatg aagaccctaa tgtgtaagaa cgtatcccta tagaatattt ggatgtgttt
     4081 ttatcaatag taatgtattt tctagcatta agtgtatgaa ctataggaaa gatgctactt
     4141 tttggagcat tgataacatc tgctatttct gtaagagtat atccattttc ggattctgct
     4201 aatgcttcta aaatatctaa tatcctagct gttgggcgat gattatctga agtcataaat
     4261 ttccccctct ttttattaat ttaaagtatt agaatatgtt gattttagca cactatacga
     4321 tattttacaa tgtagaaaat aaatatgtaa atttatatat aaataagctc ttgatttaga
     4381 tacagataga tattttttta gactgtacat taaggaataa ctaatttcca aaatatacct
     4441 aaagaaagta agtgatattt agttatataa tttaagtaaa tatatgcaat agttattttt
     4501 atatgtaaat atcaaaatat tatgaactta tattttatct attttttaca ctgtataaaa
     4561 actttatagt tttgaatact atataatttt aaatacgctg tatttttgca tataaggaag
     4621 tcatttgata atatacaagg tattatcaga aagtttgaaa aaattttgac aaaaatttat
     4681 tttttttatt gactcataaa taggaaaatg ctatcattaa ttcaaagatt cgtaattaaa
     4741 aacttagttc ttaaatacga accgaaacgg ccgttagaat taaagctaaa aacttaataa
     4801 gagtaattaa atatcatgtt ttaggaggat taactttatg ataatggatt ttattaaaaa
     4861 aattcctgca ggtatgatga tagtgccaat gtttatagca gctttcatta ataccttttg
     4921 cccagatatt gtacaaatag gttcgtttac gacagcagta ttttcaagtg ttgggtctgc
     4981 aactataatt ggggttcaac tggtatgctt gggaactcag cttcaattta gagaaatgcc
     5041 aaaagtttta aagagaggtg gtatacttct tggttctaaa tttgctatag gtgctttgat
     5101 aggtatactt ataggaaagc tatttggaat ggatggtgtc cttggtctga caacattggc
     5161 tgttataagt gcagttacaa atagtaatgg tagtatatat ctttcattga tgacttcata
     5221 tggagatgaa acagattgtg caactatgag tttacttact ttaaatgatg gaccattttt
     5281 tacattaata gctcttggga cttcaggtct tgcaaatgta ccattgttgt cattattggc
     5341 tgcaatagtg ccaatacttg ttggtatgat aataggaaat tttgacaaaa aaatgaaaga
     5401 gtttttagag ccagggtgta atttactcgt accttttgta gggtttgcac ttggtagtag
     5461 cataaatctt gcaagtatac taaaaggagg tttatcagga gtattacttg gattaatatc
     5521 tgtatttgta ggaggtatat ttatagtatt ctgtgataaa tttataggaa aacgtccagg
     5581 atatgcagga tgggctgtag ctacaactgc tggaaatgca attgctgtac ctgctgctgt
     5641 tgcattagtt gacccatcat ggcaaccata tgttgcaaca gctacaacac aagtggcagc
     5701 cgcagtagtt gtcactgcaa tattagtacc tcttataact agttggtggg ctaagaagta
     5761 tggatgtcct aagttcccaa aacaaaatga ggatggtaca ataacagctt aatttatatt
     5821 acagtgagtt gttggtaaaa taataaattc tttaattaga ttttaggata ttaataaaat
     5881 tatattattt ataaaggaga gcaagaataa tgataaatgc agtgataatt gatgaaaaag
     5941 ataatgttgc agtagctatt gaagcaatag ctaaaggtag tgaaattagt tttaaattaa
     6001 aagacaaaac agtaaaaacg attacagcac tagatgatat aactatttat cataaattgg
     6061 ctataaaaga tatggccaaa ggtgataaag ttactaaata tggtgaacat ataggagaag
     6121 caaatcagga aataaaaagt ggtcaacatg tacatattca taatgttaga agtgttagag
     6181 aggatttgga taaacaagtt atgactaata tataaaataa tatttataga gttataaaag
     6241 ttgtattcat tgcttttagt gtcaaatagt ttttaaatag gtttttagct gtttgattag
     6301 tgagtgtaat aaaaattata atatataaaa attatagaaa gtatatagac tattttagga
     6361 ggaaatcagt atgaaatttt atggatataa aagaccagat ggaagagttg gtataagaaa
     6421 ccacatatta atattgccta cgagtgtgtg tgcatcagat acaactagaa taatagcatc
     6481 acaagttcaa ggggcagtta cctttaataa tcagaatgga tgttcacaag tacattcaga
     6541 ccaacaatta actatggatg ttatggctgg ttatgcagca aatccaaatg tatatggtgt
     6601 aatagttgta tctttgggat gtgaaaattg ccaaaatgat ttggtagttg atgcaataaa
     6661 agagagaaca aataagccaa taaaaactct tgtaatacaa gaagaacatg gaactttaaa
     6721 aactatagaa aaagctgttc gttatgctag agaaatggca caagaagctt ctttgcttat
     6781 aaaagaagaa tttccaatat ctgaacttat actaggaact gaatgtggtg ggtctgaccc
     6841 aacaagtgga ttagcagcaa atccattaat aggtgaatta agtgataaac ttgtcgattt
     6901 aggagcaaca tcaattttaa gtgaaactac tgaatttata ggtgcagagc atatactagc
     6961 taggagagca gtaaatgaag aagtaaaaga aaaaatatta catatagtac atagatatga
     7021 aaattcatta aaattggttg gagaagaagt tagagaaggt aatccatctc ctggaaatat
     7081 agctggtggt ttaacaagtt tagaagaaaa atcacttggt tgtatacata aaggtggtca
     7141 tagacaaata agtgaagtat atgattatgc caaacaaatt gataagaaag gtcttgttat
     7201 aatggatacc cctggaaatg atgcttcatc tgttgctggt atggtagcag gaggagcaca
     7261 aatagtggta tttagtacag gaagaggaac accatcagga aatccaatag caccagtaat
     7321 aaaaataact ggaaataaaa taacttttgc aaatatggaa gataatatgg attttgatgc
     7381 tagtccagtt atatatggtc cacaaacaat ggaagaatta acagatgatt tgttaaatat
     7441 ggttgtagat gtagcaaatg gaaaacaatc aaaagcagag tcccttggat atactgaaat
     7501 ggcaatagct agagtatgca actatgtata atctttaagt aagaaaataa atacatagaa
     7561 ttttaataaa gggtgagatt atgaagtttg taagttttac tgaaggagaa aatgaatgta
     7621 aaagaactgg agttttttct aaagatgaat tatttattat agatgttaac tctttaaatc
     7681 tcagtagaaa ttttaaggat ttaaatgaat taattcaaaa tgtatctagt gaagatatta
     7741 ttaagttgaa aactattata aatgaaagta cattctataa ttatgatgtt tttaaaagag
     7801 agagatgtac tgtacatgtt cctatagaaa aaccaataca tgatatctta tgtgtgggag
     7861 tgaactataa agaccatctt gaagaaactc aaactcactt tgatgatagt tttgaagaac
     7921 cacaaaaaac ggtttatttt acaaaacgtg tgacaaaggc aataggtcca gaagatgaga
     7981 tagatggtca ttttgagata aataatcaac tagactatga agttgaactt ggagtagtaa
     8041 taggtaaaac aggtgttaat attagaaaag aagaggtaga agattatatc tttggttaca
     8101 ctgttataaa tgatatttct gctagaaagt tgcaaaaaga acatgttcaa tggtttagag
     8161 gaaaaggatt agatacattt acctctttag gtccatgtat tttacataaa agtgcagttc
     8221 cttttcctat aaaacttaaa gtatgtagta gggtaaatgg tgaagaaaga caaagttcta
     8281 atacaggatt attcatatca gacgtttcag acatagtttc agaactttca aaaggtatga
     8341 ccttagaggc tggagacatt atagctactg gaacaccttc tggagtaggg atgggatttt
     8401 cacctcctcg atatatgagt agtggagatg tagttgaatg tgagattgag agtattggaa
     8461 ttttaaaaaa ttacattaag taactgtttt aatataatta atattaaagc taaatctgat
     8521 gtattaatga acatattaaa ataaatttta ggagtttaaa ttatgaaagc agaatttttg
     8581 acaccagttg ttacaatatt tgatgaagaa ggaaatttag ataaagaagg taataaaaaa
     8641 gtgtatgacc atcttataga tggaggtgta gatggactag tcataatggg aagtactgga
     8701 gagttcttta atatgtctat ggatatgcaa aaagaactta tagatttagt aactagtcat
     8761 gtaaataaga gagtaaaagt gtttataggt acaagtcgta tgtgtataaa tgaatctata
     8821 gaactttcaa actatgcaca taatagtgga gcagatggtg ttatgataat aagcccttat
     8881 tatttctctc ttgatgatga tagtattgag ttattttata gtgaaatagc taagaataca
     8941 gaggctgaaa tatatttgta caattatcca gatagaactg gttatgattt aagtccagaa
     9001 cttacattaa gacttgcaag aaaacataaa aacatagttg gatataagga tacagtaact
     9061 ttgatgggac ataccagaga tttaataaat actatgaaaa tagagttccc agaatttaag
     9121 gtttatagcg gttatgatga aaactttata cataatctaa tgtctggggg agcaggttgt
     9181 atagggggat tatcaaattt aattccagaa gtttgctcca aatgggctga agcttacaat
     9241 aatagagata tggagaaggt tatagaaatt caaaaatata ttaataaagc aatggatttt
     9301 tacttaataa ccaatccatt tataccagca atgaaaaaag ctatgaatat cagagggtta
     9361 caaataagtg attatatgac tgctccattt gtaaaaccaa atgaaaaaca agtatgtgaa
     9421 attaaaaaat tgatgaatga attaaatata tgttaaatca accttgcaaa gaggtcaaca
     9481 ataaattaca attatcttaa taaaaattaa attaaaataa tactagggta ctatattagt
     9541 ttaataggga aagtgatgaa aattcactac agcccccgct actgtatacg gatacgaaat
     9601 caaaatccac tgaaatttat aaaaattttg ggaagggtga tggaggaaaa gccgtgagtc
     9661 aggagacctg cccagtatta tataagttca atttagggtg gagattgtaa tttataataa
     9721 aatgaagtat caattcgttt ttgtaaagtt gatatatgaa cttctcctta ttaaattagg
     9781 agaagttttt tattttatta aaaggttata cttattaaag atgactttag gataacttta
     9841 ataagtatac tttaaaaagc atatattatt aaagtttttt agccactcat aaaaaataag
     9901 atattggagt gaaagagatg aaatttaaat caaatgtttt aaagcagttt tttttaattg
     9961 ctatggtaat ttttatggta ggaggtctta tagggtgtag taaaagtgac aaagagaata
    10021 aacaagctag tactggtaag aaattagaat taaaatatgc tagtggattt agtgttgaag
    10081 atttagaaga tggaattaaa aaagtaactg atggagataa tcgagaactg attttaatcc
    10141 caaagaagat taaaattcct gaaaaatata aaaatgctaa tatagttaga acgcctgtgg
    10201 acaatgtatt aatagcatct acaacacagg cttgttctct gagaacaata gatgaactag
    10261 atagtataaa agcagtaaca acagaagagc agtactggac tattaaagaa attaaagatt
    10321 taatgaaaga tggaaaaata cattacgttg gttcaaattt agcaccagat tatgaaaaaa
    10381 tagtagactt aaatcctgac ttaactatag ttagtagtgg tttatcagag gctgatacaa
    10441 aatttattga caaattaaaa gaattagggc ttaattatgt tgtagacaat gagtataaag
    10501 agcaaaatcc atttggaaga atggagtgga caagtttaat agctgcattt tatgataaag
    10561 aagatatggc tactagtgaa ctaaataaaa ctgtacaaaa ggtagaagaa gtaagtaaaa
    10621 aaataaaaaa caaagctaaa cctaaagttg tatgggcatc tgtatatgaa ggaagtgctt
    10681 atatagctcc taaagattca tatgtggata atatgataaa aatggctggt ggaataaatg
    10741 catgtgcttc aatagattca aatgaagggc gagtaagtat tgaacaattg catgaagttg
    10801 caaaagatgc agatatattt gtatactcat caagttcaga ttatgcacca aatttagatt
    10861 ctataaaatc tagtgcacct gtattggctg atttggatgt tgtaaaaaac ggaaacctat
    10921 gggtgtttgc tccagattat tatcaatcag tagataagac agacgaatta atagtagatt
    10981 tagttgagat gttccaccca ggtactacag gtgaaaaaat caaacattat ataaattatg
    11041 ataaatagta cagagaggac acaaaaagat aggttttata gattttagtt aatatagctt
    11101 tgctaagagg agtatacaaa tggattttat caataaaatg agaataagga atacgacaat
    11161 atttatagta ttaattataa gtatgatact tactttttta gtgtgtgtag catttggctc
    11221 tgtaaaaatt gaatttaaag aagtaataga tgttgtatta agaggtatgg ataatacaga
    11281 acattataaa ataatataca atataagaat ccctagagta cttgcaacca tggtaggtgg
    11341 agcatgccta gcagtatcag gtattttatt acagatattt tttaaaaacc caatagttga
    11401 accgtatata ttaggagtat cttcgggttc tactttatca gtagctctca tgattttggg
    11461 aggatacaca ttgggatttg agagtgtaaa ttcaatgagt atatttgtag cagcttttat
    11521 aggagcttta attgtaatgt ctatagtcat tttatttgca aataaggtaa agaatataac
    11581 aactctttta ataataggaa ttatgaccgg atatgtatgt agtggtataa caaatgtgtt
    11641 acaatcattt gcaaatagtg ataaattaaa agaatttact atgtggtcta tgggaagttt
    11701 ttctggattt acatgggaaa aggtagaaat acttataata gttggtatat taggtctttt
    11761 gatgactgta ttcataataa aacctttaaa tgctttttta ctaggagaag agtatgcaaa
    11821 aagtattggc ataaatataa gattttttag agttttgata gtatttatat ctagtatatt
    11881 agcagcaata gtatcagcat ttgcaggacc tatagggttt attggacttg cagtgccaca
    11941 aatttcaaaa atgatattta aaacatcaga taatagagtt ttgataccag tagtaatatt
    12001 tttaggcagt atagttacag ctctttgtga tttaatatca agagtaattt tatcaccagt
    12061 agaattacca ataagtacga taacatcttt attaggagct ccaattgtag taatgttatt
    12121 ggtgaaaagg aaggataggg cttagacatg ttgaaaacta ataatttaag tgtaggatac
    12181 aataacaaag ttgttataag taatataaat gtagaagtaa aaaatggaga aatactatgt
    12241 cttttgggaa gtaatggagc tggaaaaact accctattaa ggtccttatc taaacttata
    12301 tcaccaataa aaggtgaaat atatttgaat ggtgtaaaca taaattgtat atcaagaaaa
    12361 gcattatcaa aaaaaatggc tttagtactt acaaatagat tattaggtga tttaatgact
    12421 gtacaagata tagtcaatat aggaaggtat ccatatacag gattttttgg gagtctatct
    12481 aagaaagatt taataatggt agatgaagca ttggagtctg tagatgcctt acatttaaag
    12541 aaaagatatt ttgatgaact aagtgatgga gaaaaacaaa aggtgttggt tgcaagggct
    12601 cttgttcaag aaccagaaat aattatatta gatgagccta caactcactt ggatataaaa
    12661 catagattgg aactcataaa catactgaaa aaattaagta aagaaaagtc aatatcagta
    12721 atactttcat tgcatgaaat agacatagca ttaaaaagtt gtgataaagt tgctcttata
    12781 aaaaataata aagtcatagc ttatggtcag cctgaagatg tagtagatga ggatattata
    12841 aattcgcttt atgaattgga tgataaaaac tttaatagtc ttttaggttc agtagaaatt
    12901 agtaataaat ctaaaaatga agtttttata attggtggtg ggggtaaagc aacaccaatt
    12961 tatagagctt ttactaaaaa aggtataggt ctgtattctg gaataataca tgaaaatgat
    13021 attgattatg aaattggtag aactatgggg ataaagatgt ttacagaaaa tccatttgag
    13081 cctataagtg atgagagttt cgatttggct attagaaatt taaatgattc caagattata
    13141 atagatacag gatttagtgt aggagagaca aataagagga atatagatat tataaaagag
    13201 gctttaaaat tggataaaaa agtttattct tttagaaata gagatgagag taagaagtat
    13261 tatgacagtt tggatagtaa gatagaacat atagataaag tttctcaaat aatcaatagt
    13321 gcagatataa ataatttatt ataatttatg aaataaaatg tacttatgtg ctatagaaaa
    13381 aagagtatag attataattt ttttgaaatt acaatctata ctcttttgtt attttgttta
    13441 tgtatttata tatttataaa actttagcat tttatagata accaatagct tcctctctag
    13501 ttaaaaaaaa gtgaatccct gtagtcgatt ctttccatct attttcatta aaatcttcag
    13561 ctacaatcgt ttctcctact ctatatataa aattttcatc agcatatgca cgagcacttt
    13621 taaaagattc tttaccatct atactagtaa ttgacagtac ttttgcctta tcacatctac
    13681 aagcgtttga tgtagcagac actctttttg cgtcgctggg tattaataat tgtaccattc
    13741 taaaattaaa acatttttta tagcctataa atgctcctgt ttcaggacaa tacaatttaa
    13801 aaaaacgggt actttcatct gatattactc catttaaagt agcatgttct aaatttgaat
    13861 tttctaaatt agctcctcta atatctgagt tagataagtc acaaaatcta aaacttgcac
    13921 cacttaagtc tgcatttttt aatatagaat tttttaatga acaatccgaa agataagaat
    13981 tattaaaaat agagttttct aaatctgctt tttctaaatt taaattgata aggtcagacc
    14041 acgaaaagtc aatattactt aagcagtatc ttcttaagtc catattttct aatcttaatt
    14101 cttttaaatc tgccttttct ccagcattat tgtttttaag ccataaataa tgtttctcaa
    14161 gtgttttttc taattcttgt ttagaaatag ttttcattaa aatcaccttc ataaatttat
    14221 ttttatatat gtagtagcaa aatagtcata tatttaatta tataacaata aataaaaaac
    14281 ttctaaataa tatataaaat atcatcttga aagtactttt ttcaatatat cagaatatat
    14341 tttta
//
LOCUS       DAEMIK010000114        14270 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEMIK010000114 DAEMIK010000000
VERSION     DAEMIK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.115"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..342
                     /locus_tag="KPK87_002777"
     CDS             <1..342
                     /locus_tag="KPK87_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538910.1"
                     /translation="LFKSFMYVKSINELEDYISQNKLVDKSKNTLPTTDKTREKFSKI
                     KLNEIKKIYETIGINSITKISIDKNKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVR
                     DGEYYRFRIKS"
     gene            357..941
                     /locus_tag="KPK87_002778"
     CDS             357..941
                     /locus_tag="KPK87_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538911.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1194..2231
                     /locus_tag="KPK87_002779"
     CDS             1194..2231
                     /locus_tag="KPK87_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG2538912.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2312..2677
                     /locus_tag="KPK87_002780"
     CDS             2312..2677
                     /locus_tag="KPK87_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538913.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2682..2999
                     /locus_tag="KPK87_002781"
     CDS             2682..2999
                     /locus_tag="KPK87_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538914.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3047..4651
                     /locus_tag="KPK87_002782"
     CDS             3047..4651
                     /locus_tag="KPK87_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538915.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4775..6403
                     /locus_tag="KPK87_002783"
     CDS             4775..6403
                     /locus_tag="KPK87_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG2538916.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6668..8017
                     /locus_tag="KPK87_002784"
     CDS             6668..8017
                     /locus_tag="KPK87_002784"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG2538917.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8173..9252
                     /locus_tag="KPK87_002785"
     CDS             8173..9252
                     /locus_tag="KPK87_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG2538918.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9597..10505
                     /locus_tag="KPK87_002786"
     CDS             9597..10505
                     /locus_tag="KPK87_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG2538919.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            10510..12879
                     /locus_tag="KPK87_002787"
     CDS             10510..12879
                     /locus_tag="KPK87_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG2538920.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13204..14007
                     /gene="proC"
                     /locus_tag="KPK87_002788"
     CDS             13204..14007
                     /gene="proC"
                     /locus_tag="KPK87_002788"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG2538921.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5846 a         1460 c         2628 g         4336 t
ORIGIN      
        1 ttatttaaat catttatgta tgtgaaatct ataaatgaac ttgaagatta tataagtcaa
       61 aataagctag tggataaatc aaagaacact ttaccaacaa cagataaaac tagagagaaa
      121 ttttctaaaa taaaacttaa tgaaataaaa aaaatatatg aaacaattgg aataaatagt
      181 attactaaaa tatcaataga taaaaataag ctagagatgg aaggtgtgtg tagtgattta
      241 gatgttttaa acaatttaag gaaaataaag tacataaaat attattacat agacagtatt
      301 gtaagagatg gagaatatta tagatttaga ataaaaagtt agatgggtga ttatttttga
      361 gattaaataa tagagaaaaa aatctactta aaatattgtt aatattagtt attatattgg
      421 caagtgatat agcagtaagt tatttggaag aaaacctaac tagtttgaag aatgaaaagc
      481 aaaatataat aaataaaata aataatcaag ataattatca aataaaggat aataagcata
      541 tagatgataa atatatagaa agtgttgaat taaataaaaa tgaagaattg aaaaataatt
      601 ttaataactc atttttagat aaaaaaacaa ataaagatat agtgttatta gtagaaaatg
      661 aactgagaga tattgtgaat gtagaatata ttaatcaaga aagtagttta gataatcaca
      721 ataaaaaaca agttaattta gaaataaaag ttaatggaga acttgcaaat gtgttgaaac
      781 ttgaaggtgt aatgaagaag ttaagactaa ataataaact tgaaaagata gaaattaaca
      841 aattaaaagt caataatttt gatgttcaaa atattccaaa tgaatataaa caagaaaaag
      901 acaatattgt tgaatgtaaa atgacagtta gagtcatata acaaaaaaga atagataaag
      961 taaaaaacta ttatcaatta ataaaatttt atagaggaat tagttttacg ataattcatt
     1021 tctacaagag ggattgttga tgattttatc gaaatcctaa ttaaagttaa tatattgtac
     1081 caaaatataa gtgaatttaa tagaatattt acttatacta tagattgatt aataaaaaaa
     1141 tggtataata acaaattgta ctttaattta ataataaggg agtggaagtc atattgttag
     1201 ggagaaatga gctatgccct tgtggaagtg gcaaaaaata caagaaatgt tgtttaaata
     1261 aagatattgt ttcagaaaga gcatgcagaa aaactgaact ttcacaaaaa caatatacag
     1321 atttatattc aaagttatat gattattcaa gacaggataa gtttaaagaa gaatgtgaaa
     1381 aagctaaaga aatgttttat ataatgcaaa atgatagtgt aaatgaaaaa tttgaaagat
     1441 tttttaatac ttatttaata caagaccaca taatggaaaa taaaaaggtt atgacagttg
     1501 gttttcttga agaaaatggt gcaaacctaa gtcaatcaga agtatcaata ttaaaaagta
     1561 tgtttgaatc atacgtaagt atttatgagg taaaagaagt ttctgctgga aaaatagtat
     1621 taaaggactg tttaagtgga aatgaacttt gtacagaaga tgtaaaactt ctgagaagtt
     1681 ttaaaatcgg aagttgtatg atagctagaa tagttgatat tgaaggaact aacatattga
     1741 tagatataac tataagtata tcaaatgagg ttaaagatat aattttaaag gatattatga
     1801 atctattcaa tcaatatcaa gatgtataca aagatatgaa gtcgttctta atttatcata
     1861 cacatatact atacaagtat atacaacagc ttttagaccc aagtatagct gaatatttaa
     1921 agagagaaag agataaaaac gatacaaaag ttaattcaga aaatgaatta gctgaagaag
     1981 aatgtaaagt tttagatact cttaaagaaa atgtagaaaa agaaaatata gatgcatgta
     2041 tagagttttg gaatgaatat aagaaaaatc atgaaaatat aaaaggagct gaaaatggtt
     2101 gggcagctgc tgttgaatac tatgttaaaa aagaagcagg acaacctata actcaagttc
     2161 aaatatcaaa gaaatacgat ataagctcta gtacactagg aaagagatac aaggatttaa
     2221 aaatatctta attaattata aaaagcttta agtcatagat aaagaatcta tagtataata
     2281 tatgtataat gtttagaaag aggtattaat aatgagtctt agtttaggaa atgcaattgc
     2341 tgtatcaacg aactttataa aatctaaccc agctgtacct gtgtacaacg gagaagaaat
     2401 atatgcagtt atttatcata tagaaatagg atataataat ggtgtatcac ctgataccaa
     2461 acaatatata ataatagact ataaagagtt tgatttatct atggataaaa aaaatgaact
     2521 aataaatatt gctaaagagt actgtaaatc taaaggtatt gtagaaaacg aaaaccttga
     2581 aataaacctt ttagacaatg aagaagcaga gaacttctta gaaaaggtgt ttaataatat
     2641 gaaggttata agaggtctta taactaaaaa aggataaata aatggactat ataggaatag
     2701 aaaatataac accttatgaa aatacatatg aatttagtgt atatgaatat gatgatgaaa
     2761 tcaccttagg tagtgaaaag ttatatgtat gtgaattaag ggttgtattg attaaagtta
     2821 attctctgta tgttgaaaga ttgcataaat cagttgaagc aatggtctta gtaaaaaact
     2881 tgaaaaaaga tttagataaa acacttgttg taaacaaaat aaagaatttt gtgctagatg
     2941 agatttgggt agaaaatcta gtaaaagaga atatagaagt tatatttgta gaaagctagg
     3001 aataacccta gcttttttat attacaatct aagagaggaa aacaatatgt taagagtatc
     3061 taatgtaaaa cttggaattg ataaagatat atctttatta aaagatgtta tacttaagaa
     3121 gctaaaaata aaagaaaaaa acttagtcaa atatagtata tataaagaat ctatagatgc
     3181 tagaaaaaaa ggaaaaatgg aatttgttta ttctgtagat gtagaagtta aagatgaaaa
     3241 taaaatactt aactcaaaaa taaaagatgt aacaaaaata aaggaaatca gatacgtaaa
     3301 tgtacctgtg ggtgataaaa aattaaagaa taatcctttg gtaattggta gtggaccagc
     3361 tggcttgttt gcaggtctgc ttttagctca aatgggttat gaaccaatca tacttgaaag
     3421 aggtcttgat gtagacagta gaaccaaaga tataagtgat ttttggacaa ctagaaaatt
     3481 aaaaaataat tcaaacgttc aatttggaga aggtggagca ggaacttttt cggatggtaa
     3541 acttacaact agaataaaag atatacgatg tagaaaagta ttagaagagt tagttcagtt
     3601 tggagcacca gatgaaatat tatactcaca taagccacat gttggaacag atatattaaa
     3661 aaatgtagtt aaaaatataa gaaatgaaat aataagttta ggtggtcaag ttagatttaa
     3721 ttcaaaagta acagatatag ttgtatcaaa tgattctata caatctgtaa taataaatga
     3781 tactgaaaaa atagatacag atacaattat attagctgtt ggacatagtg caagagatac
     3841 ttatgaaatg ttgtattcaa gaggtataaa aatagttcaa aaaccttttg caataggagc
     3901 tagagttgaa catccacaag atttaataaa taaagcacag tacaaggatt tttataatca
     3961 tcctaggtta ggagctgcag attatagact tatagagcat gtttcaaatg gaagaacagc
     4021 atatactttt tgtatgtgtc caggaggtag tgtcatagca tctgcatcaa atgattttga
     4081 aatagttaca aatggtatga gtgaacatgc aagagataaa ataaatgcaa atagtgcatt
     4141 tttagtaaat gtaacgccag aagattttaa gagtgataat ccactagcag gtgttcattt
     4201 ccagcagaag tatgaaaaat tagcttttga attaggtgga aaaaattata atgcaccagt
     4261 tcagcttgtt ggagatttta taaacaataa gacttctttg aatttaggaa gtgttgagcc
     4321 atcatataga ccaggatata cacttacaaa tttaagtgaa tgtttgccta gcttcgtaac
     4381 agagactatg agagaagcgt ttatatctct tgataaaaaa ttgaatgggt ttgcaatgta
     4441 tgatgctgtt ttgacgggag tagaaactag gtcatcatca ccaataagaa tagtaagaga
     4501 tgaaaatact ttagaatcag tgagtattaa gaatctatat ccatgtggtg aaggtgctgg
     4561 atatgcaggt ggtatagtta cagcagcagt tgatggtata aaatgtgcag aaaaaataat
     4621 acaaaaatac accaataata caaaaattta acataaactt aatgattttt taatatttaa
     4681 catgaattta acattatcac tcctataatt tgataaaata caataggtta taaaattttt
     4741 agatacagta atctctttac aaggaggaaa taatttgaac attataataa gtctaatggg
     4801 tggattaggt ctgttcttat atggtatgaa ccttatggga gaaggtcttc aaaaatctgc
     4861 tgggacaaag ctaaaaaaga taataaaact acttactagt aacctattta tgggagtttt
     4921 ggtaggtaca ggagttacag cagtaataca aagttcaagt gctactactg taatggttgt
     4981 tggtttcgta aatgcaggaa taatgacttt aaaacaggca ataggagtta taatgggtgc
     5041 taatataggg actactgtta cagcccaatt ggtatctttc gacttaactg gtatggcccc
     5101 agtagcatta ggtgtaggta ttatacttta tttatttgga aataagccta gaataaaaaa
     5161 tatagccgaa atacttatag gttttggtat attatttaca ggtatggatt ttatgaagat
     5221 ggcagtggag ccactaagag actatcaagg atttactgac ttacttgtta catttggtag
     5281 atatccacta cttggattat tattgggatt tgggattaca gcaataatac aaagttcaag
     5341 tgcatctatg ggtatgttgg ttgcactggc agcagaaggt ctagtgccat tatcagcagc
     5401 actgccaatt ctttatggcc aaaatattgg tacatgtgta acttcactgt tatcaagtat
     5461 tggtgctaac aaaaatgcac gaagagcagc tatgatgcat ctaatcttca atgttttagg
     5521 aacagtgata ttcttaatat tcttgaataa accagtagta agcatggtta caagttggga
     5581 cccaagcaat gttgcaagac aaatagccaa tacacataca ttgtttaata taatatctgt
     5641 attgatactt ttaccattta caaatcttat tataaaacta gctataaagc ttgttcctga
     5701 tagagcaggt gatatagatg aagatgaaac taaaactatt aaatatatag atgatagaat
     5761 gatagaaact ccttctatag ctcttgcaaa tacgataaaa gaagcacttc gtatgggtga
     5821 gaaggcgaag gaaagcctaa atgcatctat ggaagcttta gtagaacatt caactgaaaa
     5881 aatagataag acatataggc gagagagatt aatcaatgat ttacagaaag ctattttaaa
     5941 ttatttactt aaactttcaa aagcaccttt agatgatgat tcaagagaag ttgtggatac
     6001 tttgtttaat acagtcaatg atatagaaag aattggtgac cacgctaaaa atatagctga
     6061 attatcgcaa gttgcaatag attcaaatat ttctttctca gaagaaggac aatctgaact
     6121 tgatgttatg tataatagag ttgtttcagc atatacttat gcattagaat caatgagaac
     6181 tgataatgtt gatttagctt gtaaggttat aaaaattgaa gaacaagttg atataatgga
     6241 gaaatcttgt agagctaatc acatgtatag attgaacaat aatctatgta gtatagaaaa
     6301 tggagttatt tatttagacg taataagtaa tttagagaga atatctgacc atgctgtcaa
     6361 tatagcacaa caagttatag caaagagatt aggaaatgat taaaaataat taatttaaaa
     6421 taatataaaa tttaaaatag aggataaata aaagagaaac tttaaatgat acgtttagag
     6481 tttctttttt tgtaaattta tttgactaaa atttttaaat ttatacatag ttatagcatt
     6541 tagaagaatc gatttcctga taataaatga tgagatatgt atgattttgt ggcaaacttt
     6601 taaaaaaaac acctatattt tgtgttataa tctctttaaa acgaatatgt tgaaaagagg
     6661 gatttaaatg ggaaaaataa attttgatta tagtaaagct acagagtttt tttgtcaaaa
     6721 cgaaatagat gtcatgcaac catatgtaga tgttgcgcac gacatgttac ataataaaac
     6781 tggtttagga aatacttttt taggatggat agaccttcct aaaaattatg ataaagaaga
     6841 gtttgacaga ataaaaaaat ctgctgagaa gataaaatca gactcagatg ttcttttggt
     6901 aataggtata ggaggctctt atttaggttc gagagctgct atagatatgg taagtcattc
     6961 ttttaggaat ggacttaaaa aagagcaaag aaaggctcca gaagtatatt ttgtagggca
     7021 taatataagc tcaacatata ttatggattt attagatata attgaaggaa aggatatttc
     7081 tgtaaatgta atatctaaat caggaactac tacagaacca gctctggctt ttagaatatt
     7141 taaagattac ttagagaaaa agtatggtaa ggaagaagca agaaagagaa tttatgcaac
     7201 aacagatgca agtaaaggtg cacttagaca gttagctaca gaagaaggat atgagacttt
     7261 tgtaatacca gatgatgtag gtggtcgttt ttctgtattg acagcagttg ggttacttcc
     7321 tattgctgct gctggacttg atattgatgc tatgatgaaa ggcgcaaatg atgctagaga
     7381 agcatttcaa aatcctgact taaaaagtaa tgatagttat agatatgctg ttgctagaac
     7441 aattttacat agaaaaggaa aagatgtaga gttattagtg aactatgaac ctcaactaca
     7501 ttatgtaagt gagtggtgga aacagcttta tggagaaagt gaaggaaaag aaaataaagg
     7561 tttattccct gcttctgtag atttctcaac agatttacat tctatgggtc aatacataca
     7621 agatggaaaa aggcttttat ttgagacagt acttaatgtt gaaaactgta agagaaatat
     7681 aactataagt tcagaggaag tagacttaga tggtttaaat tatttagcag gtaagactgt
     7741 agattttgta aatcataaag cttttgaagg aactctattg gctcatacag atggaaaagt
     7801 acctaattta gtaataaata tacctcaact agatgagtat aattttggat atttagttta
     7861 tttctttgag aaggcttgtg gaataagtgg atatttattg ggagttaatc catttgacca
     7921 accaggagta gaagcttaca agaaaaatat gtttgcttta ctaggcaaac caggttatga
     7981 aaaagaaaag gaagagctag aaaaaagact taaataattt taaatagtta aggtttaatt
     8041 aagattaaaa taatataatg aaatcatcaa aaaggagttt actcaaaata gatgatttca
     8101 ttttttttat gaaaaattgg gaaaaatata ttttgataat ttatattata ttaagatagg
     8161 gagtggtatg agatgtcaag aagaaagaaa gggataagtt tagtaatatt agttgctata
     8221 ataagttcaa tacttagtag ttttctaaca ataattttag ttaaggacaa tttagtaagc
     8281 aaatctacag gaagttctac gccgatagtt gtaaatgatg atggtaagag tcaaaatatt
     8341 tatcaagcag ttgcagaaaa agctacgcca tctgttgtag gtattacaac aactagtgtt
     8401 gatactagta atatgtttgc aataccaact gaaacacaag gtgtaggaac tggaatcata
     8461 gttgattcaa atggatatat acttactaat tctcatgtta tatcagatgg tcaagctacg
     8521 agtgtaaatg tattattcaa tgatggttca acaacttcag gtaaagtagt atggtttgac
     8581 caacaacttg atttagctat agttaaggtt gataaaactg gactaacacc agcagaattt
     8641 gctgatagtg acaaagtaaa agttggagat atatcaatag caataggtaa tcctcttgga
     8701 ttagattttc aaaaaacagt tactcaaggg ataataagtg gattagatag aactatacaa
     8761 acagaaaaaa ctaatatgac tggtttatta caaactgatg caagtataaa tgcaggaaat
     8821 agtggaggac cattgttaaa tcaaaaaggt caagtaattg gtataaacac agcaaaagct
     8881 tctcaagcag aaggattagg atttgcaata ccaatcaata cagctaagag tatagttgaa
     8941 gaagtaatca aaaatggaaa atatgaaaaa gtaactttag gcataaaagg tacagatgta
     9001 tcaaattatg aagcagcaac aggtacaaaa ttaagtactg ataaaggtgt ttatgttgca
     9061 gaagtaatat ctggttcatc agcagaaaaa gctggagtaa aggtagggga tattataact
     9121 aaagtaggag atacagatat aacaggtatg aatgatttaa ataaaaagtt gtatactttc
     9181 tcaaaaggtg ctagtacaaa aataacagta aatagaggcg gaaaagcagt aacaataaat
     9241 gtgaatttct aaatatgacc agaaagatag agactacatg gtaaagtgta gtctctattt
     9301 ttatttaatt tttatagtat atacatctaa attaattaaa atgatgtatt gtttatagct
     9361 tattgctata ttttcttata aaaataaatt taataaatat ttaaatttaa tttacaaaaa
     9421 caatataaat atcataataa aataaaattt tttaatatta ctaaaaatat atagacaaaa
     9481 taataacaaa tatagtatta tgtaattgta gaaatgcttt tcataggtat attctatgaa
     9541 gatgaattgc ataaaaatga aaaaatggaa tggatattat ttaggggtga aaaatcatga
     9601 atccattagt tataaactta caaaaatgta gcatacatga tggaccaggg ataagaagta
     9661 cagtattttt taaagggtgt ccacttgaat gtgtttggtg tcataatcca gagagtcaaa
     9721 catatacaaa acaagttttg tataatgaag aaagatgttc aaaatgtgaa gcatgtataa
     9781 atatatgccc tcataaggca atttacaaag gtgaaacaaa aatatgctta gaccaagata
     9841 agtgtgagtt ttgtgaaact tgcttagact attgtgtaaa caatgcaaga gagattgttg
     9901 gtcaagagta ttctgtaaga gatttggtta aagagataga gaaggataga attttctatg
     9961 aagaatcagg tggtggagta actctatcag gtggagaagt aatggctcaa gatatggact
    10021 tcatttgtgg agtaataaac atgtgcaaat caaagggaat ccatgtagca attgatacat
    10081 gtggctatgc aaagtcagaa aattatgaaa gagttgctaa atgtgcagat ttgtttttat
    10141 atgatattaa gcttattgat gaagataagc acataaaatt tacaggaaaa tcaaatgact
    10201 taatactgaa aaatgttaag attttaagtg aattaggggc taacataaat ataagaatac
    10261 cattaattgt aggagtaaat gtagatgatg aaaatttaga ggttaaaaaa atgatagagt
    10321 ttttaaagcc tttgaatata caagcagtaa gtcttcttcc atatcataat attggtaaac
    10381 acaagtatga taaaatttat aaaaagtatg aaggagaaga actacaaaga ccatcagaag
    10441 aaaaattaga ggaaataaaa agactgtttg aagcaagtaa tttcaatact aagattggag
    10501 ggtaataaaa tggcaagagg aacttttgag agaactaaaa aattaagaga agaaagtatt
    10561 aatgcagaac cacacatatc tattgaaaga gcagtactta tgacagaagc ttataaaaaa
    10621 tatgagggaa gtgtagagat tcctgtactt cgtgctttat catttaagca ttatattgag
    10681 aatagaactc ttagcataaa tgatggagaa ttaatagtag gtgaaaaagg tgattctcct
    10741 aatggagcac caacatatcc agaaatttgt tgtcatacaa tggaagattt agaagtaatg
    10801 cacaatagag atataataaa ttttagcgta tcagaagaag ctagaaaaat acataaagaa
    10861 gaaataattc ctttttggaa aaagagacaa acaagagata agataataaa tgcaatgaca
    10921 cctgagtggt tagcagcata tgaagcaggt atgtttacag agtttatgga acaaagagcc
    10981 ccaggacata cagtttgtgg agatacaata tataaaaaag gatttttaga tttaaagaaa
    11041 gacatagaag caagattaaa agagctagat tttcttaatg atttagatgc atataataaa
    11101 aaagcagatt tagaagctat ggcaatagct tgtgatgcaa tggttatatt aggtaaaaga
    11161 tatgctgaga aagcaagaca aatggcagaa gaagaaacag atgaagctaa gaaaaaagat
    11221 ttattactaa ttgctgaaac ttgtgatgtt gtaccagctc acaaaccaga aacttatcat
    11281 caagctatac aaatgtattg gtttgttcat ataggggtta ctactgaact taatatatgg
    11341 gatgctttta ctccaggaag acttgaccaa catctaaatc ctttctatga aagagatgta
    11401 gaaaatggaa tactagatag agatagagca caagaattac ttgaatgttt atgggttaaa
    11461 tttaacaatc agccagcacc accaaaagtt ggtataacat taaaagaaag tagcacatat
    11521 acagattttg caaatataaa cactggtgga ataaatccag atggtcaaga tggtgttaat
    11581 gaagttagtt atataatact tgatgttatg gatgaaatga aattgataca acctagttca
    11641 aatgttcaaa taagtaaaaa gactcctcaa aaattcttaa agagagcttg tgaaatatca
    11701 agaaaaggat ggggtcaacc agcattttat aatactgaag caatagttca agagcttatg
    11761 gaagctggaa aaactataga agatgcaaga cttggtggaa ccagtggttg tgttgaaact
    11821 ggatgttttg gtaaagaagc atatgttcta actggatata tgaacattcc aaaaatactt
    11881 gaattaacat taaataatgg atatgaccca attagtaaaa aacaaatagg tattgaaact
    11941 ggagacccta gaaatttcca atcttatgag gaactatttg aagcatttaa aaagcaatta
    12001 cattatatga ttgacataaa aatagaagga aatgctgtga tagaaaatat atgtgcaaaa
    12061 cacatgcctt gtccacttat gtctacaata gttgatgatt gtatagaaaa aggcaaggat
    12121 taccaaagag gtggagcaag atacaacact agatatatac aaggtgttgg tataggaact
    12181 ataacagata gtttaacagc aataaaatat aatgtatttg ataagaaaaa attcgatatg
    12241 gatacattgc ttaaagcact tgatgctaat tttgaaggat atgaagctat acttaattta
    12301 gtttcaaata agactcctaa atatggaaat gatgatgatt atgctgatga aataatgcaa
    12361 gagatattca atgcatatta caatgaggta actggtagac caactgtttg tggtggagaa
    12421 tacagagtag atatgttacc aacaacttgt catatatact ttggagaaat tatgggagca
    12481 agtccaaatg gtagactttg tgcaaaacca gtttcagagg gaatttcacc tgaaaaaggt
    12541 ggagatacta atggacctac agcagttata aaatcttgtg ctaagatgga ccacataaaa
    12601 actggtggaa cattattaaa tcaaagattt gcaccatcag tagttcaagg agaaaaaggg
    12661 ttagataata tggctaatct agtaagagca tactttaata tggatggtca tcatatacaa
    12721 tttaatgtat ttgataaaaa tgtactgtta gaggctcaaa agaatcctca ggattataaa
    12781 gacttaatag ttagagttgc aggatatagt gaccatttca ataatttaag tagaacacta
    12841 caagatgaga taataggaag aactgagcaa acattctagg tttgcttgta aataaaactt
    12901 cttactatat aaaatcgtaa ctgatataat aaagtataaa tggtttgatt ccatttttaa
    12961 aattttagat taattatgag ctaactttct aaataggaag ttagctcgtt aagtttaatg
    13021 aaaaaatcgt taaatttata tttagtaaat catacacata ttagtaagta atacatacaa
    13081 aagaataatt tattatagtt ataataaggt tatattttat aaaaataaga taattttaag
    13141 cagttaaaat aaagtcaatt tagctacttt aaaataaaac taatagaaaa aaggagattt
    13201 gatatgaaaa ctttaggatt tattggttca gggaatatgg gaagtgcaat gataggtggc
    13261 atagtacagt caggattagt aaaggcagaa gatataactg tatcagattt aagccaagtt
    13321 gccttagata aagtaaaaga agaatttgga gtaaatacta caactgattc aaaggtagct
    13381 gttacaaatt ctgatgtggt aatagtagca cttaaaccta atatatgcga aaaggcttta
    13441 actccactta aagaattaat agatgcaaat aaaataatag tatctatagc agcaggtaaa
    13501 actatagaaa gtctggaaag ttttataggt tcagataaaa aaatagtaag agtaatgcct
    13561 aatactccag cccttgttgg cgaaggaatg tcagctcttt gtaaaaatag taatgtaaca
    13621 gatgaagagt taaatatgat taaggctctg tttgaatctt ttggtgaggc agaaatagta
    13681 tcagaatatt taatggatac agttacaggt attagtggtt cttctcctgc atatgtattt
    13741 atgtttatag aagctatggc agatgcagca gtattagctg gaatgccaag acaacaagct
    13801 tataaatttg catctcaagc agtaatgggg tcagctaaga tggttcttga aactggaaaa
    13861 catccaggtg aattaaaaga tatggtttgt tctccagcgg gaactactat agaagctgtt
    13921 aaggtattag aagaagaagg atttagagca gcagttataa aatctattgt tgcttgtata
    13981 gataagtcta aagatatgag taaataacag atgtaattaa gtttactata tggtttcttg
    14041 aattgttttt ttagtgctta tttatatcta taaataaatg tatttggtat aagtaaaata
    14101 agagtaattt actataaaaa ggaatttaat gattgagtga gaatttatta cttaaatatt
    14161 aataattaaa tagatatatt tttaactata tttgagatat atcattgtga aaaaataaaa
    14221 tttataagta ttattgtgag gttataagta gatttattta aaaataaatt
//
LOCUS       DAEMIK010000115        14210 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEMIK010000115 DAEMIK010000000
VERSION     DAEMIK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.116"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..197
                     /locus_tag="KPK87_002789"
     CDS             <1..197
                     /locus_tag="KPK87_002789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538922.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="KPK87_002790"
     CDS             314..811
                     /locus_tag="KPK87_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2538923.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="KPK87_002791"
     CDS             786..1292
                     /locus_tag="KPK87_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2538924.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..3723
                     /locus_tag="KPK87_002792"
     CDS             1276..3723
                     /locus_tag="KPK87_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538925.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3726..5903
                     /locus_tag="KPK87_002793"
     CDS             3726..5903
                     /locus_tag="KPK87_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2538926.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            5900..6901
                     /locus_tag="KPK87_002794"
     CDS             5900..6901
                     /locus_tag="KPK87_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2538927.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            6898..7830
                     /locus_tag="KPK87_002795"
     CDS             6898..7830
                     /locus_tag="KPK87_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2538928.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            8082..10001
                     /gene="tet(M)"
                     /locus_tag="KPK87_002796"
     CDS             8082..10001
                     /gene="tet(M)"
                     /locus_tag="KPK87_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBG2538929.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10120..10287
                     /locus_tag="KPK87_002797"
     CDS             10120..10287
                     /locus_tag="KPK87_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG2538930.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(10347..10700)
                     /locus_tag="KPK87_002798"
     CDS             complement(10347..10700)
                     /locus_tag="KPK87_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538931.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            11205..11627
                     /locus_tag="KPK87_002799"
     CDS             11205..11627
                     /locus_tag="KPK87_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2538932.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            11624..11854
                     /locus_tag="KPK87_002800"
     CDS             11624..11854
                     /locus_tag="KPK87_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2538933.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            12315..12518
                     /locus_tag="KPK87_002801"
     CDS             12315..12518
                     /locus_tag="KPK87_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2538934.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            12600..13817
                     /locus_tag="KPK87_002802"
     CDS             12600..13817
                     /locus_tag="KPK87_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2538935.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         4656 a         2527 c         2908 g         4119 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc agaacgtggc agatggaaag aaaccttgcc agagatttcc
     2821 catgaaatca atatcgtcac tctgacttct gatgagaaaa acaaaggctt acttgaccct
     2881 tatgtgatta tgaaaaatcc caaagattct gaatcactgg ctattgatat tctgacattc
     2941 cttacgggga tttcctctcg tgatggggaa cgcttcccaa tccttagaaa agccattcgt
     3001 gcagtaacca atagtgaagt acgagggttg atgaaagtga ttgaggaatt acgggttgag
     3061 aatacgccac taagtaccag tatagccgac catatcgaaa gttttacaga ctatgacttt
     3121 gcacatttat tattcagtaa tggttatgtg gagcagtcta tcagcttaga aaaacaactg
     3181 aacattatac aggttgcgga cttggtactt cccgacaagg aaacttcctt tgaggaatat
     3241 accactatgg agcttttatc cgttgctatg ctgattgtca ttagtacctt tgctttagac
     3301 tttatccata cagaccgaag cattttcaag attgtagatt tagacgaagc atggagcttt
     3361 ttacaggtag cacaaggaaa aacactatct atgaagctgg ttcgggctgg tcgtgctatg
     3421 aacgctgggg tatatttcgt gacccaaaat acagacgacc tcttagatga aaaactgaaa
     3481 aataacctcg gcttaaaatt tgcatttcgt tccactgacc ttaacgagat taaaaagacc
     3541 ttagcctttt ttggtgtaga cccagaggac gaaaacaatc agaagcgatt gcgtgatttg
     3601 gaaaacgggc aatgccttat cagtgattta tatggtcgtg tcggtgtgat acagttccac
     3661 cctgtatttg aagaactgct ccatgccttt gataccagac cacctgtgcg aaaagaggtg
     3721 taaatgtgaa accatcaata gtaaacagaa taaaatcaaa ctggacgctg aaacgtctag
     3781 gtaaagtggc aatgacagtg gctttcacac ttgtgattgc catttttctt ttagccatgc
     3841 tgggaacggt ggttcaagct gcgggcttgg tagatgatac ggtcaatgtg gcaaatgaat
     3901 acagccgata cccacttgaa aactatcaac tggattttta tgtggataat agctggggct
     3961 ggcttccgtg gaactggtcg gacgggattg gaaaacaggt catgtatgga ctatatgcca
     4021 ttaccaattt tatttggaca atcagtttgt atgtttccaa tgcgacaggt tacttagtac
     4081 aggaagccta ttccttagac ttcatttccg ctacagcaga ttccattggt aagaatatgc
     4141 agaccttagc tggtgtgagt gcaaacggat tttcaacaga gggtttctat gttggattcc
     4201 tcttactctt gattttggtt cttggggttt atgttgccta tacgggactg ataaagagag
     4261 aaaccacaaa ggcaattcat gccattatga attttgtgct ggtgtttatc ctatcggctt
     4321 cctttattgc ctacgctccc gactacatta aaaaaatcaa tgacttttca tcagacatca
     4381 gtaatgccag tttatcactt ggcacgaaga ttgtcatgcc ccattccgat agtcaaggca
     4441 aggacagcgt ggacttaatc agagatagcc tgttttccat acaggttcag caaccgtggc
     4501 tactgcttca atacaacagt tcagacattg aaagtatcgg tattgaccgt gtggaaagcc
     4561 tgctctccac cagcccagat tccaacaatg gcgaagacag agaaaaaatt gttgcggaag
     4621 aaattgaaga cagaagcaat accaatctaa ccattacaaa gaccattaac cgtttaggta
     4681 cagtcttctt cctatttgtc ttcaatattg ggatttccat atttgtattc ctattaacag
     4741 gaatcatgat tttctcgcag gtacttttta tcatctatgc tatgtttctg cctgtgagct
     4801 ttattttaag catgattcca tcatttgatg gtatgtcaaa acgagccata acaaagctct
     4861 ttaataccat tttgacacga gctggaatca cattgattat tacgacagca tttagtattt
     4921 caaccatgct ctatacctta tcggctggtt atccgttctt tttgattgct tttctacaga
     4981 ttgtgacctt tgcaggaatc tacttcaagc tgggcgattt aatgagtatg ttttctctac
     5041 agagtaacga ttctcaaagt gtgggaagtc gtgtgatgag aaaacctcgt atgcttatgc
     5101 acgctcacat gcaccgtcta cagcggaaac ttggacgttc catgactact ctaggggctg
     5161 ggtctgccat tgttacaggt aaaaaaggac agtcgggttc ggggagttct gcaaggacac
     5221 aagcagatca ctcccgacca gacggaaagg aaaaatcaac acttggaaaa cgtatcggtc
     5281 aaaccatcgg tacagtagct gataccaaag acagaatggt agacactgct agtggtttga
     5341 aagaacaggt taaagatttg ccgaccaatg caagatatgc agtatatcaa ggaaaatcca
     5401 aagtaaaaga gaatgtccgt gatttaacca gtagtatttc tcaaaccaaa gcggacagag
     5461 ccagtggacg caaggaacag caggaacaaa ggcgaaaaac cattgcgaag cgtcgctctg
     5521 aaatggaaca ggtcaaacag aaaaaacagc ctgcttcttc tgttcatgaa agaccgacta
     5581 caagacaaga acaatatcat gatgaacaga cctcaaaaca gtctaatatt cagacttcat
     5641 ataaggaatc tcaacaagcc aaacaagagc gtccagcagt taagtccgat ttttcaagtc
     5701 caaaagtgga acgccaaggc aataccgttc aagaaaaaac cgttcaaaag ccagcaactt
     5761 caaccactac agcagataga acttcacaac gtccaatcac aaaagaacgt ccgtctactg
     5821 ttcaaagagt accactacaa aatacaagaa gtagaccacc aatcaaaacc gccaccatta
     5881 agaaagtcgg taagaaacca tgaagttgaa aactttagtg attggtggtt ctggattatt
     5941 cttgatggtc ttctcactgc ttctgtttgt tgccatttta ttttcagatg aacaggacag
     6001 cggaatttcc aatattcatt atggaggtgt gaatgtttcc gcagaagtgc tggctcataa
     6061 gcctatggta gaaaaatatg ccaaagaata tggcgttgaa gaatatgtca acatacttct
     6121 tgcgattata caggtggaat cgggcggtac tgcggaagat gttatgcagt cctcggaatc
     6181 cctcggtctt ccacctaatt cattgagtac agaagaatcc attaagcaag gtgtgaagta
     6241 tttcagtgaa ttattagcca gtagcgaaag gctcagtgta gatttagaat cggttatcca
     6301 gtcctacaat tatggtggtg gtttcttagg gtatgtggct aatcgtggaa ataaatatac
     6361 ctttgaactg gctcaaagtt tctcaaaaga gtattcaggt ggcgaaaaag tgtcttaccc
     6421 caatcccata gccataccta tcaatggggg ctggcgatac aactatggca atatgtttta
     6481 tgtgcaactg gtaacgcagt atcttgtcac aacagagttt gatgatgata cggtacaagc
     6541 catcatggac gaagcactga aatatgaggg ctggcgatac gtttacggtg gagcttcccc
     6601 gactacttct tttgattgta gcggactgac acaatggacg tatggaaaag ctggaattaa
     6661 cttaccacga accgcacaac agcaatatga tgtgacccag catatcccac tatcggaagc
     6721 acaagctggc gatttggttt tctttcattc tacctataac gctggctctt atattactca
     6781 tgttgggata taccttggca ataaccgtat gtttcatgca ggcgacccaa tcggttatgc
     6841 cgacttaaca agcccctact ggcaacagca tttagtggga gcaggacgaa tcaaacaatg
     6901 agaaaggaag atttaatgat gaaatttaga aaaaatcaga ataaagaaaa acagatacca
     6961 aaggaaaaga aacctcgtgt ctataaggtc aatcctcata aaaaggttgt gattgccttg
     7021 tgggtacttt tagggcttag tttcagcttt gcgatattca agcactttac agctatagat
     7081 actcatacta ttcacgaaac aactatcata gaaaaggaat acgttgatac tcatcatgta
     7141 gaaaattttg tagagaactt tgcgaaagtc tactattcat gggagcaatc cgataagtcc
     7201 attgataatc gaatggaaag tctaaaaggc tatctgacag atgaacttca agctctcaat
     7261 gttgatacag tacgcaaaga tattcctgta tcgtcttctg taagaggatt tcagatatgg
     7321 acggtagagc caactggcga caatgagttt aatgtaacct acagtgtaga ccagctcatt
     7381 acagagggag aaaatacaaa gaccgtccac tctgcttata tagtgagtgt ctatgtagat
     7441 ggttctggaa atatggtact ggttaagaat ccgaccatta ccaacatacc taagaaatca
     7501 agttataaac caaaagccat tgaaagtgag gggacggttg attccattac aaccaatgaa
     7561 atcaatgagt ttttaacgac gttcttcaag ctctatccta cagcgacagc cagtgaactt
     7621 tcctactatg tgaatgacgg gatattaaaa ccaatcggaa aagagtacat ctttcaagaa
     7681 ctggtaaatc ctattcacaa tcgtaaggat aatcaagtca cggtatcgct gacagtggag
     7741 tatatcgacc agcagaccaa agcaacgcag gtatctcaat ttgatttggt acttgaaaag
     7801 aacgggagta attggaagat tgtaaaataa caaatattgg tacatgatta cagatacttt
     7861 gtaatcatgt actctttttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt
     7921 gctattattt aagtgactat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa
     7981 taaactgtca atttgatagc gggaacaaat aattagatgt ccttttttag gagggcttag
     8041 ttttttatgc ccttttgggt ttttgaatgg aggaaaatca catgaaaatt attaatattg
     8101 gagttttagc tcatgttgat gcaggaaaaa ctaccttaac agaaagctta ttatataaca
     8161 gtggagcgat tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc
     8221 ttttagaacg tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa
     8281 atacgaaggt gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc
     8341 gttcattatc agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag
     8401 cacaaactcg tatattattt catgcactta ggaaaatggg gattcccaca atctttttta
     8461 tcaataagat tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga
     8521 aactttctgc cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga
     8581 cgaactttac cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag
     8641 agaaatatat gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa
     8701 gatttcagaa ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga
     8761 ttgataacct tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt
     8821 ctgaactttg cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat
     8881 atatacgcct ttatagtgga gtactacatt tacgagattc ggttagagta tcagaaaaag
     8941 aaaaaataaa agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata
     9001 gagcttattc tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc
     9061 ttggagatac aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac
     9121 aaacaactgt tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg
     9181 aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta
     9241 tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt
     9301 atcatgtgga gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa
     9361 atgcagaata taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt
     9421 tatctgtatc accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg
     9481 gatacttaaa tcaatcattt caaaatgcag ttatggaagg gatacgctat ggctgtgaac
     9541 aaggattgta tggttggaat gtgacggact gtaaaatctg ttttaagtat ggcttatact
     9601 atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta ttggaacaag
     9661 tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc
     9721 cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag
     9781 acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc
     9841 aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt
     9901 taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc
     9961 ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata
    10021 taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt
    10081 gtaaaggagg agatagttat tataaactac aagtggatat tgtgtcctgt atgtggaaat
    10141 aaaacacgat taaagataag ggaagatact gaattaaaaa aattccccct ctattgtccg
    10201 aaatgcagac aagaaaattt aattgaaata aagcagttca aagtaactgt gattacagag
    10261 ccagacgcaa agacgcagag ccgataaaat gagattaata caatctcatt ttatcggctc
    10321 tttccgttat gtatggattc ttttaattag tcttcgatgt ttcttgcttc gttgataccg
    10381 ctggctaaag attccattaa ggatagttct ttgtctgtaa agctatccat gtatttctct
    10441 atctgtaatc gtcgggtgct ttttaccaag ttattagcag gtaagaaaaa ttcatcaacg
    10501 gaaacatgaa gtaacgatac aaggtcataa agaacttgta tgctggggtg ttgcccttta
    10561 ttttcaatat tagttaagta ccgtgggtca atttcaatca atgctcccac ttgttcacga
    10621 gttaaacctc gtttcaatcg agcttcttta atggctaaac caaaggctct aaaatcatat
    10681 ttatcttctt ttttacgcat agtagaccac ctctatacat tttattgttc ctactgaatt
    10741 aaaaacaggt atagaaaaac gtgttatatg gtttataggt ttatatttaa taaaaagcac
    10801 tactaaacgc caataaaaaa aaccgttata tggtagtgct atttacgctg ttaaaatatt
    10861 gtatattact tccaaatggc ggtttgttgg aggtcaacgt cgccatgaag tacatcatat
    10921 acaataaatt tccttacatt gggttcttgt caaaaaaagt cgtctatctg caatagataa
    10981 gtacgtccac caatgtggtt ttataaatca tatagataga ataacagaag catgtaaaca
    11041 gagaaataaa tctgtttata tgcttttttg gctattcaga acttttttac aaagtttatt
    11101 tatcagtaat gcaacaaatc cccctttcac attgggacta agagtgaaag gagataaacg
    11161 agcaaggctc acttcctttc ctagacagaa agggggtgag aaacatgaaa ccatcttctt
    11221 ttcagaccac aatagaaaat cagtttgact atatctgtaa acgtgctatg gaagacgagc
    11281 gaaagaatta tatgctttat ctttcaagga ttgcaaagcg tgaggtgtcc ttttcggatg
    11341 ttggcgatta tcttgttagc cagtttgcga caacagataa ctattcaact gactttcaga
    11401 tttttacact caatgggtta tcagtaggcg ttgaaaatga tttgttgagt gaagcattac
    11461 gtgagttgcc agacaagaaa cgtgaaattc tactgctgtt ttactttatg gacatgagcg
    11521 attcagaaat tgcagacctg ttgaaattga accgttctac tgtctatcgg catagaacca
    11581 gtggactagc cttaattaaa aagtttatgg aggaatttga agaatgaaaa cacaatatcc
    11641 tatgattccc tttcctctca ttgtaaaggc aacagatggc gataccgaag cgattaacca
    11701 gattctacat cattacagag ggtacataac gaagcgttcc ctacgactta tgaaagatga
    11761 atatggcaat caaagtatgg tcgttgatga agtcttacgt ggaagaatgg aaaccagact
    11821 gattacaaag attttgtcat ttgaaattaa gtaatatcct ctctcctttc gtggaagcgt
    11881 gctaaaccat tccacgcttc ccgaacaggg aggtttgtta ttccaccaaa gcatattgag
    11941 ctttcaatgt gttttgatag gctaacgagc cattgttctt tgaaaactga ataaaagtaa
    12001 tcgaatacgt ttcgataaga aaagagccaa cggaactaac cgccatgacc tatcttataa
    12061 agatagcgag cgattcatgt tagtgatccg agaagcaatc tttagcagga ttgcctgcaa
    12121 cgacattctt atcgtgataa tgatactccc atacagtcaa tagtccgagc gtgataaaac
    12181 cgtcgcaggc aatgagtatg gctacatgag aaccatgcag gggtggaact cccgtgagct
    12241 ttgctaaagc tgttcgattg ctggtaaaac aacttttatg aaatccaaat aagtgatttg
    12301 gaaaggagga ttttatgaag cagactgaca ttcctatttg ggaacgttat accctaacca
    12361 ttgaagaagc gtcaaaatat tttcgtattg gcgaaaacaa gctacgacgc ttggcagagg
    12421 aaaataaaaa tgcaaattgg ctgattatga atggcaatcg tattcagatt aaacgaaaac
    12481 aatttgaaaa aattatagat acattggacg caatctagcg tcgccaaagg gtcttgtata
    12541 tgataaaata gtattaagtc gtatcaaggc tctttccata aaggaaagga gcaaatgcca
    12601 tgtcagaaaa aagacgtgac aataaaggtc gaatcttaaa gactggagag agccaacgaa
    12661 aagacggaag atacttatac aaatatatag attcatttgg agaaccgcaa tttgtttact
    12721 cgtggaaact tgtggctaca gaccgagtac cagcaggaaa gcgtgattgt atctcactta
    12781 gagagaaaat cgcagagtta cagaaagaca ttcatgatgg tattgatgtt gtaggaaaga
    12841 aaatgacact ctgccagctt tacgcaaaac agaacgctca aagaccaaag gttagaaaaa
    12901 acactgaaac tggacgcaaa tatcttatgg atattttgaa gaaagacaag ttaggtgtaa
    12961 gaagtattga cagtattaag ccatcagacg ctaaagaatg ggctattaga atgagtgaaa
    13021 atggttatgc ttatcaaacc atcaataact acaaacgttc tttaaaggct tcattctata
    13081 ttgctataca agatgattgt gttcggaaga atccatttga ctttcaactg aaagcagttc
    13141 ttgatgatga tactgtccct aagaccgtac taacagaaga acaggaagaa aaactgttag
    13201 cctttgcaaa agctgataaa acctacagca aaaattatga tgaaattctg atactcttaa
    13261 aaacaggtct tcgtatttca gagtttggtg gtttgacact tccagattta gattttgaga
    13321 atcgtcttgt caatatagac catcagctat tgagagatac tgaaattggg tactacattg
    13381 aaacaccaaa gaccaaaagt ggcgaacgtc aagttcctat ggttgaagaa gcctatcaag
    13441 catttaagcg agtgttagcg aatcgaaaga atgataagcg tgttgagatt gatggatata
    13501 gtgatttcct ctttcttaat agaaagaact atccaaaagt ggcaagtgat tacaacggca
    13561 tgatgaaagg tcttgttaag aaatacaata agtataacga ggataaattg ccacacatca
    13621 ctccacatag tttgcgacat acattctgta ccaactatgc aaatgcagga atgaatccaa
    13681 aggcattaca gtacattatg ggacatgcta atatagccat gacgctgaac tattacgcac
    13741 atgcaacatt cgattctgca atggcagaaa tgaaacgctt gaataaagag aagcaacagg
    13801 agcgtcttgt tgcttagtag tacaaatgaa tttactactt atttaccact tctgacagct
    13861 aagacatgag gaaatatgca aagaaacgtg aagtatcttc ctacagtaaa aatactcgaa
    13921 agcacataga ataaggcttt acgagcattt aagaaaatat aaaaagataa ttagaaattt
    13981 atactttgtt taatataaaa attttattaa atttagtatg taatttttta atcttatttt
    14041 attccatcta tagtcttatg ctaaataata actttgataa tatttcccta acttatgttt
    14101 caactggttt atattgcaat ttatttaatt acagcttatt tgcataataa ttcgtccata
    14161 tcataagttg tgtatttata taaatacaca attaaaaata aatcactatg
//
LOCUS       DAEMIK010000116        14156 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEMIK010000116 DAEMIK010000000
VERSION     DAEMIK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.117"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            325..3228
                     /locus_tag="KPK87_002803"
     CDS             325..3228
                     /locus_tag="KPK87_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2538936.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEAEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLLPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYKISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3261..3707
                     /locus_tag="KPK87_002804"
     CDS             3261..3707
                     /locus_tag="KPK87_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2538937.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3830..4132
                     /locus_tag="KPK87_002805"
     CDS             3830..4132
                     /locus_tag="KPK87_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2538938.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4313..5719
                     /locus_tag="KPK87_002806"
     CDS             4313..5719
                     /locus_tag="KPK87_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2538939.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKHLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6063..7121
                     /locus_tag="KPK87_002807"
     CDS             6063..7121
                     /locus_tag="KPK87_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2538940.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7220..7906
                     /gene="araD"
                     /locus_tag="KPK87_002808"
     CDS             7220..7906
                     /gene="araD"
                     /locus_tag="KPK87_002808"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG2538941.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7924..8571
                     /locus_tag="KPK87_002809"
     CDS             7924..8571
                     /locus_tag="KPK87_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG2538942.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKAIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8744..10111)
                     /locus_tag="KPK87_002810"
     CDS             complement(8744..10111)
                     /locus_tag="KPK87_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG2538943.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10301..10389)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10817..12937
                     /locus_tag="KPK87_002811"
     CDS             10817..12937
                     /locus_tag="KPK87_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG2538944.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKYLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13041..>14156
                     /locus_tag="KPK87_002812"
     CDS             13041..>14156
                     /locus_tag="KPK87_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG2538945.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKE"
BASE COUNT         5640 a         1594 c         2483 g         4439 t
ORIGIN      
        1 taaaatagaa agggttaaaa acacagttta ttataatttt tattgttgag tagatacgta
       61 ctattatata tgattgagca agtttttcaa tcataaattt ataaagtatt ttgcattaaa
      121 atttaaacaa agagttaaat tatttaagat tatgaataac taatacattt agtaaattac
      181 taaatataca aaatgtaaat acttatcaaa atgtataaat acttatgaca tctagttact
      241 tacagggttt aagtagaaaa aaatatcaaa gtaagtatgg catgatagtt gcttatatat
      301 ttatcaagaa aggagttatt aagcatggag ttgagagata aacttgtaaa catcataaat
      361 acagaaaata aaaaagatcc attaacagat gttcaaatag caaagttttt aagtacaact
      421 agagagaaca taactaattt gagaaaagaa cttaatataa gtaattcaag gcaaagaaga
      481 tatccttatt taaaatcagc aatttcggca atattacaaa agaataagaa tatttctata
      541 tcagaaatta caagggaatt gatgacagaa ggctttaata tatcgagaag agttgtggag
      601 gaacttttac ctaaagaaag tttagaaatg gatgtagtag aggaagctga agaagaatcg
      661 aatgacccat ttgaaactct gataggtaat aaagggagtc ttagaaatgc tgtagagcag
      721 gctaaatctg ctatattgta tccaccaaaa gggttaccaa cgttgattat tggggaaagt
      781 ggagttggta agagtttatt ttctagacat atgtatgagt ttgcaaagca aaaaaagata
      841 gtaaaagaga gctccaattt tgttgtattt aactgtgctg attatagtga caatccacaa
      901 ttattattat ctttgttatt tggatataaa aaaggtgcat ttacaggtgc tgaatatgat
      961 acaccaggtc ttgtagaaga agctgatgaa ggagttttat ttttggatga aatacataga
     1021 ttgcctccaa aaggtcaaga aatactattt tctatcttag ataggggaaa gtttaggaga
     1081 ttaggtgaaa ctaatgctga aagaaaagta agtattatgt tgattggggc aactactgaa
     1141 aatgtagaga ctaatttatt attaacattc agaagaagga taccaatgct aataactttg
     1201 ctaccattac atgatagatt actaaaagag aaaatagacc ttatatacaa tatctttcaa
     1261 caagagtgta atagaataaa tgcaaaaata tttgtagata aaaatgtgat tgaaatactt
     1321 gcactaaaaa agtttagtgg aaatatagga caattacaaa atatgattca ggttctttgt
     1381 gcaagagctt ttatgaagtt tataaattcc aaagatgaag atagtgaatc tatagtagta
     1441 gtagatataa atgaagtttt aaagcttaaa gattctttta aagatgctgc ctttcaagag
     1501 attgaatata cagagataag aaaatatttg aaaaatgcta tatttatacc atttaattta
     1561 gaagaatcat cagtaaaagg aagtctttta agtgatgaat atatattacc agaggacata
     1621 tacaaaacaa ttgaaaagaa atattatgac ctaaaatccc tagacattga agatatagat
     1681 atagagaata ttttatggac ttttatttta aatagatttg ctgacttaaa gtttaatctt
     1741 gatagtaatg aagaaatttt ttcaatgagt gatttaaata gttttgtaag tgatagttta
     1801 gttaaactgg taaaagaact tatgaatgat atagttgaaa aaaatatgaa tttagaagta
     1861 aataaaaata tgttcaaata tctagcaata cacttagaag aagctataaa aagaattaag
     1921 ctaaatcaaa agataataaa tgtaaattta gaaaaaataa agattgactt ttcaaaagaa
     1981 tataaaatta gtaaaagttt tgcaataaaa attgaggaat caattggtat taagatttca
     2041 gatgatgaaa ttggatttat aacgctctac ataaagtctg ctctaaaaaa tgaagtgaaa
     2101 aaagataaag tagggttaat aataatttct catggacgta tagctacaga aactgtaaat
     2161 gtagttaaag aacttttagg tgtaaagttt cctgtggcta tagatatgcc tttagatgaa
     2221 aaaccaataa atatatacaa taaggcagta gaactgtcaa aaataataga ccaagggaaa
     2281 ggcatattgt ttttggtgga tattggttca cttacaaaca taggtcaaat agtaaataag
     2341 agaaccaata taagtactaa aactattgat agagtagacc tcctaatggc actagaagca
     2401 actagaaaag tttctatggg agaggaagaa cttgatgaaa tatttttttc tttgctaaaa
     2461 gataggatgg gatataacta tgaattagaa aaacacatag ataaatcaaa tgcaattata
     2521 acattatgtc ttactggaga aggaactgct aagtatataa gtaaaacgtt ggaagaaaag
     2581 tatgataaca ctaaatgtta tcaaatgagt gcattggatg aaaatttatt ttgtaaaatt
     2641 gaaaatctaa aagagacaaa caatatatta gctattgttg ggacaataaa ccctaagata
     2701 ccaggtataa attttatacc ctataataaa gaacttttca aaaatttgga tatctatcta
     2761 tcaaaatcaa aacaacaaga tgacagacca attaaatcgt atgaaagaat gcttgatgag
     2821 gatttagtga tttttgaacc agatatatat tttaaaaagg atttattaga atatgtatgt
     2881 tcaattctta taaataaaga atatgtagaa aaagattact tagattctgt tttacataga
     2941 gaagaaatgc ttccaacata ttctaaagga gctataggtg tgcctcatgg agattcttct
     3001 actgtaaata gtacaaggtt tgtttttgtt aaactaaaga atcctattga ttggggtgta
     3061 ggtaatgtaa atttcatatt tatgccagtt tttcaggcta atgataaaga gattgttaaa
     3121 aatgttcttg agatactaaa agatgcagaa tttatgaaca atgccaataa gtgttttgat
     3181 aaagatgctt ttagaaggat tatatttgat aaatttaaac atctgtagcc atagcaaaaa
     3241 aattgagagg agttagagta atgaatgtag atttaatatc attgggttta gaatctaatg
     3301 attatgaaag tgttattgaa gaattaggaa gtataatgtg taaaaaagaa tatgtaaaag
     3361 aaacatacat aaatgctgtc ttggaaagag aaaggacatt accaacaggt ctagatattg
     3421 gagaaatgtg tgttgctatt ccacatacag attcaaagca tgtaaatgag tctaatgtag
     3481 ctgtagggat attgaaaaat cctgtaaagt ttaattcaat gatagaccca aaagatagac
     3541 ttgatgtaga acttgtattt ttattagcag taaagaaccc agactcacaa gtaaaactgt
     3601 taaaagattt aatgtcagtt tttcaaaata taaagttatt aaaaaatata aaaaacgcat
     3661 caacaaaaga agaagtagct aaattattgg actttataga aatttaatgt aggagaattt
     3721 tataaataaa aaattaaaat ttaaacaatc aaagtaagta ttattttttt aataggttta
     3781 atcttatata aaataaaaat ttagagtgaa aaactaggag gaaattatta tgaaacaatt
     3841 taatgtatta tcagtttgtg gttcagggac agttacatca tctatggtag caggtaaatt
     3901 aaaagaagta ctaggtaata aggggatttc tattactaca acagaagcta gaccaacaga
     3961 agcactaaat ttagcacaat caggaagatt tgactttata acatttacga gtccacttca
     4021 aacaggcgat tatggaatac caactataaa tgcatttgct tgcctaactg gtatagggga
     4081 agaggcattc tttgaagaag ttttgactgt tattgaaggg atagataagt aagttttgga
     4141 gacgtgaatt tatagtattt aaatcaatga tttagatact ataataagct aatattaact
     4201 aaatatttgt ggaaaaaaca ttttatatgt tgtcataaat taaaaaatta taaaaatagt
     4261 cacttaagta ttaaattaag cttataaata tttataagta ggagggaaaa agatgttaga
     4321 atttatgaaa tctataatcg atacatttgg ttctgcaatt atagttccaa ttataatatt
     4381 cataatagca aagatattta aagtaactac taagaaatcg tttctttctg ctgtgtatgc
     4441 aggagttgct ttacaaggtt ttacactgat tttaaattca tttacaccaa taataacacc
     4501 agttatcaat aggatggttg agagtacagg agtaaaccta ccagtttttg atgttggttg
     4561 gcaagcaaca tcattagttg cattttcaac aagtgcaggt atgatatatt taggtcttgg
     4621 tatattatta caaacaattt tatttttgat aaaatggaca gacgtgtttc aaccatcaga
     4681 tttatggaat aactattctt atatggtatg gggagcaatg gttataggag taacaggaaa
     4741 ttttccttta ggtatagcat gtatggtttt attaaaccta tatagtttat taatatcaga
     4801 gttagttgca aagagatggt ctagttacta tagatatcct aattgtacaa taatagctat
     4861 gcataatgtt gaggcttctg tgtttgcagt atttgcagac cctatataca ataaattggg
     4921 attaaataaa ataaaattaa atccaaaaga attagagaag aaattagggt ttttaggtga
     4981 accaattacc ttaggtcttt ttttaggtat gtttataggt atattaggta atatgacgag
     5041 aataaatact atggaagctt ggggagaaat aatgaaagta ggtatatcta caagtgcagt
     5101 gatggcaatt ttccctaaag tagcaagtat gtttgctcaa gcatttgcac ctataacaga
     5161 agcagccaga aagattatgc aaaaagctgg aaaccgtgag tggtacatag cagtaaatga
     5221 tgctgttgga tatggtgaac ctgcaacttt aatatcaggt cttatattaa ttccaataat
     5281 gcttgtaata gctatggtac ttcctggtaa taaggtatta ccagttgtgg atttattggc
     5341 aattccatat atggttcaag gtttagttgc catacataat ggtaatatac caaaagtatt
     5401 agtttcaggt ataatttggt ttggattagg attatatgtt tgtacatcta cagcaccatt
     5461 atttacagat atggctacaa atataggtgt agctatacct gcaggagcaa tgttaataac
     5521 aagctttaat atactaggaa aacatttgat ggggttagtt ttctttgcat ttctaagtgc
     5581 aaatccaatt tatataggtc tttcagttgt aatttacttt gttctatggg cattgtttag
     5641 aaagaacaaa acaagtattc ttgattattt agaaaaacaa gcacttaaaa atgtagaaga
     5701 agagcctgta gcagtttaag tttataaaaa tgaaataaaa ataattaaag gagtttataa
     5761 ttgattttat ataagtatca gataatacct gagaaatatt taaatgaaat atagacttct
     5821 ttttatttga taatatttgt aattataatg cattgtatct ttatagttga gttttctgta
     5881 taacctgcta tttaaaacat actcttttat atttatacag taatgtggtt taattttata
     5941 aaatgtaatc tctactggta aaagactttg tatataaggc atttgcctga ataaaccatt
     6001 tatcttttgt atggcataga tattgctata tagaagtata tagcatgaaa ggagatattt
     6061 atatgggaaa taaaatgaga gcatcagtat tgtataatgt aggtgatgtc agatatgaaa
     6121 tggtagatat accagaaata acagatacgc aagtgcttgt aaatgtaaaa tatgttggga
     6181 tatgtggttc agatttacct aggtctatgg taagtggttt gagtggaaat acaaaatatc
     6241 cattgatttt agggcatgaa ttttctggtg aagttgttaa aattggagaa aaagtaaagc
     6301 atattaatgt aggagataga gtagctgttg caccattagt accttgtgga aaatgtgatt
     6361 attgtaatga aggtaatttt ggcttgtgtg atgattacaa tataattgga acaagagtaa
     6421 atggagcgtt tgcagaatat gtaagagttc cagaagaaca tattttaaaa ctaccagata
     6481 cattggatta tgaaactgct gccggaattg aaccagcaac tatagcttat catgggattt
     6541 caaagtctaa tattagagta ggagatagtg tagttgtgtt aggatgtggt ccaataggtc
     6601 agtttgttat acaatgggca aaggtgtttg gtgcatctaa gattatagca gttgatattt
     6661 ttgatgaaaa attagaactt tctaaacttc taggagcaaa ctatatactt aactcaaaag
     6721 aagtaaatgt aatcaaagaa ataaaaaaaa taacaaatgg tggtgctgat gttgtaatag
     6781 agactgctgg tagccgattt acacaagaac aatctctatt tgtagcaaaa aagagaggaa
     6841 atatagtgtt tgtaggtata tcacacacag aattaccact tagtgcagac gctactgagt
     6901 gtatattacg tggagaactt accttgaaag gttcttggaa ttcatataca agcccttatc
     6961 ctggaagagc atggactgca acattggatt ttatgggaaa aggtgacatt atttttaaac
     7021 ccatgatttc acataaaata ggtcttaatg aagtgggtga ctttttaagt aaaatgagca
     7081 agagagaaat taatttcaat aaaatcctag tagaaatata aaaatttaat aaaaaattct
     7141 aaaagcaatt aaattttata actatgacat taaataaaat aaaaataatt ttcttaaata
     7201 aaatatagga ggaagaaaag tgttagaaaa tttaaaagaa gaagtattaa aagcaaactt
     7261 agaattacct aagaaaaagc taattactta tacttgggga aatgtaagtg ggatagatag
     7321 agaaaaagga ttagtggtga taaaaccaag tggtgtagag tatagtaata tgactgtaga
     7381 agatatggtt gtagttgatt tagagggaaa tatagttgat ggcaaattaa gaccttcttc
     7441 agatactccg actcatctag ttttatataa agagtttgaa gaattggggg gaatagtaca
     7501 tacacattca agttgggcaa caatttgggc tcaaatagga aaatctatac catcttgtgg
     7561 tactactcat gcagattatt tttatggaag tataccatgt accagaaaaa tgactagaga
     7621 agaaatttgc aatgaatacg aaaaggaaac tggaaatgta atcattgaag aatttatggg
     7681 aagaaatcca attcattgtc caggaataat tgtaaatgac catggacctt ttacatgggg
     7741 aaaagatgca aatgaggctg tacacaatgc agtagtttta gaagaagtcg caaagatggc
     7801 ttattataca gagttaatga gtcctgataa tattatggat aaggttctta tgaataagca
     7861 tttttcaaga aaacatggaa aaaatgcata ctatggtcaa aaatgattat ctggaggtga
     7921 attatgctag actctttaaa aggtaaagtt gtggaaatag caattaaagc agagcattat
     7981 ggtttatgta aagaaaagac tggtaatttt agtattagag ataaggatac tggatatata
     8041 cttataaccc catcaggtgt aggaagagaa gaattaaaag ttgaacatat ttgtgtattg
     8101 gatttggatg gaaatgtaat agaagttgag aaaggtatta agccaagtag tgaggtattg
     8161 atgcatattg aaatatacaa aacaagagaa aatataaatg ccatatcaca tactcattct
     8221 ttatatgcaa cagcattttc agtagcaaag aaagcaatta taccaattgt atatgaatca
     8281 gttaattatg gcggttatgt agatattgct tcatacgaaa gaccaggaac tttaaaatta
     8341 gctaagagtg tttcaaaatt actgatagat acagatgcat gcttattaga aagacatggg
     8401 ttgacaactg taggtaatga tttagacgaa gcattattaa aatcaagata tgtggaagag
     8461 gtagcagaaa tatactatag gtctttagta ttaaatcaat ttaaagaact tgaagtagta
     8521 gatgttgatg agtttaaatc ttggaaatac ccagaagaaa taaagtttta atctaaataa
     8581 ttttcattga agtggttaat taaaatatat gtattattta tatcaattac tatgttttat
     8641 tatgttaatt accgtatttt gcaaatatat ttattacatt ttttcaaaaa aatatagaat
     8701 ccgaaagcca tataaaattg acttgggatt ctatactttt tatttatgca tttttcttta
     8761 tctgattttc tggagaattt atatgttcaa aatatttttt agtttcagat actattatag
     8821 gtgataatgc aagtaaagct ataaggttag gtattgccat aagcccattt acaatatcag
     8881 caattatcca aacaacttct aactttaaga aagcacctaa taacaccata gccacaaata
     8941 cagttctata cccattcatg ccttttacac caaacagaaa ttcaaaacat ctttctccat
     9001 aataactcca tcccaaaatt gtagtaaatg caaataatgt taaactaact gtaagtaata
     9061 ttggtccaaa tgtaggaatt gctccgttaa aagctgcttg tgtcataaca gctccattta
     9121 ggtctgaaca ccacactcct gaaataacaa gagagaatcc agtaagagta cagattataa
     9181 tagtatctat aaaagtacct gtcatagata taagaccttg ttccgctggc cattcagttt
     9241 tagcagcagc agcagctatt ggagcactac ctagaccaga ctcatttgaa aatacaccac
     9301 gagcgatacc atttctaatt gctagtgaca tagttgctcc taaaaaacca ccagtagcag
     9361 cagtaggtgt aaatgcactt ttaattatta acataaaagt ttcagggatt ttattccaaa
     9421 aagatattaa aatgattcca cagattacaa catatataac tgccataaaa ggtactattt
     9481 tagtagctac attagatata cttttaagtc ctccaaatac aaccaaagct acaaatactg
     9541 ctaatacaac ccctgtaatt ctagggtcta tcccgaaact attatttatt gcatctgtta
     9601 ttgaatttac ttgagtaaaa gtaccaattc ctaaaagtgc aactagtata ccacttatag
     9661 cgaaaaatat tgcaagaggt ctccattttt ctcccatacc atttactatg taatacattg
     9721 gcccaccaga cacttggcca tttttatctt tagttctgta ttttattgca agaacacctt
     9781 ctgaatactt agtagccatt ccaaagaatg ctgctatcca catccaaaaa agtgcaccag
     9841 gtccaccagc tttgatagct gtagcaacac caacaatatt accagtacca actgtagcag
     9901 ctagagcagt acataaggct gcaaaacttg aaacatcacc actacctttg ttttcagcag
     9961 agaatattaa cttaagagct cttggcagtt tagttatttg aacaactcca agcttaacag
    10021 taagtagtat accagtacca actaatagta ccagtagggg tggtccccaa ataaaagcat
    10081 caactttgtt taaaatatca attagattca taaaaacact ccctcatttt ttttgcttta
    10141 cataatcaat agaattttta atgaagtaat tgacaggaaa taagtataat ttattaataa
    10201 tttctagtgt aataaaaaaa gctaaatcta aaaaagattc agctctggat gtacactaat
    10261 tagcaaccaa aataaaagta ttagtaaata ctgtttttgg tagataatcc ctgtcctttt
    10321 gcctgagaga ttgagcataa aagccttgcc ccttcggcgc ccataatggg tctctccaga
    10381 gttccgtaaa ttttacagtc ctacttttaa aagtacctga gagtgttact ccttcggtgt
    10441 gctatatagc aactcttcta taaaatctta accggatatt gatttgtcat agcttattgt
    10501 attaataata ttaatataag tttttatgtt ttgtcaacta aaaaattaaa aaacaaagaa
    10561 aaaatataaa aatgtaagcc tatttacgaa ttataaactt agaaagatga gtaatatacg
    10621 tgaaaatgaa ttttgtaata cacatacaaa tgaagtgtaa aataagtttt aaataaaaaa
    10681 ataataaaat cttaaataat taattagata tgattaaatt atggtaatat tatagttacg
    10741 atattaagaa aagttctatt tagttagatt ttaagcgtaa tatgagatta tgtatagaga
    10801 aaggatgtat ttttagatgg gaaaaatatt agtgctagca gagaaacctt ctgttggaag
    10861 agatatagca aaagttttag ggtcaaaaaa tgaaaaaaat ggttatatag aggggccgaa
    10921 gtatgtgata acttgggcac taggtcattt ggttacactt gctgaccccg aaagttatgg
    10981 agaaagatat aaatcatgga gcttagaaga cctgccaata cttcctaagt accttaagac
    11041 tgttgtaata aaaaagagtg gaaagcagtt taatacagtt aaatcgcaaa tgaatagaaa
    11101 tgatatagat gaaatagtta tagcaacaga tgcaggtcgt gaaggagaac ttgtagcaag
    11161 gtggataata gaaaaatcac aggtaaaaaa acctataaaa cgtctttgga tttcttcaag
    11221 tacagacaaa gcaataaaag acggatttgc aaaattaaga agtggaaaag aatatgaaaa
    11281 tttgtattac tcagcaatag cacgagcaga agctgattgg attattggaa taaatgcaac
    11341 cagagctttg acaacaaagt acaatgcaca gctttcttgt ggaagagtac aaactcctac
    11401 tcttgcaatg ttgcttaaaa gagaagaaga aataagaaat ttcaaaccta aagagtatta
    11461 tggattagaa ttaattgcta caaagggaaa ttcagatata aaatttattt ggaatgataa
    11521 aaacaataat tcttcaactt ttagtaaaga aaagatagaa tccacattaa aaaaagtaaa
    11581 aggtgtagac ataaaaatat ctgaagtaaa caagtcatat aagaaaaaat attcacctgc
    11641 actctatgat ttaacagaat tacaaagaga tgcaaataaa atgtttggat tttcagcaaa
    11701 agaaacttta tctataatgc aaaaactgta tgaacatcat aaagtactta cttatcctag
    11761 aactgattct agatatttaa catcagatat tgttggaaca ttaaaagata gaataaaagc
    11821 tgtaaatatt agtgattatt caaagacttg taataaatta ttaaaaagca atattatagc
    11881 taataaatcg tttgttgaca attcaaaggt aacagaccat catgcaataa taccaacaga
    11941 acagaaggta ttcatgagtg attttagtga caaagagcga aaaatatata atcttgtagt
    12001 aaaaagattt ttagcagtac tatctcctcc ttttgaatat gagcagacaa ctataagtgc
    12061 aactgtagaa ggagaaatat ttaaggctaa gggaaacaaa gtaataaatt taggttggaa
    12121 agaaagttat aaagatattg aagatgaaga tgagtatgaa agtataccaa atgttttaga
    12181 aaaagatgta cttaaggtca agggattaaa gataacttca ggaaaaacaa atccaccacc
    12241 atacttaaat gagggaacat tattgactgc tatggaaaag aatggtctag ggacagttgc
    12301 tacaagagca gatataatag aaaaattatt caattcattt ttaatggaaa agaaaaataa
    12361 agatatatat ataacatcaa aaggaagaca attattagat ttagttccag tagacttaaa
    12421 gacaccagaa cttacagctt catgggaaaa gaaattattg gatatttcaa agggaaaact
    12481 tagtaaaaat acattcataa atgacatgaa agattattca aaagatattg tactagaagt
    12541 aaaaaatagt gataataaat ttaaacatga taacttaact aaaaatagat gtccagaatg
    12601 tggaaaattt atgttagagg taaatggtaa aaaaggtaag atgttgattt gtgaagatag
    12661 agaatgtaat actagaaaaa ctatatcaca agtaaccaat tcaagatgtc ctaactgtca
    12721 taaaaaatta gaacttcgtg gagaaggtga ggggagaatc ttcacttgta gttgcggata
    12781 cagagaaaag ttgtcttctt tcaacaagag aaagcaagaa gaaaaacaga aggcatcaaa
    12841 aaaagatgtt aatcaatatt taaaaaatca aaataaaaat caagagacat ttaataatcc
    12901 atttgcagaa gcattagcta agctaaaaaa caaataattt taaagttatt attatacaca
    12961 attataactt taactgtata ttagttatat gtactgatat aatatagtgt atagtttagg
    13021 catgaaagta gggctgttaa atggatttaa gtggcatatt taaatactat tgtaaagagt
    13081 gtgaaaatac atggaataat tcgagtgttg aattatttga gaatatagaa acgtatagta
    13141 aagattcaca aaaaaagagg gaaaaagaat tagataaatt gctaaataca atatcagttc
    13201 atttagagag gtatccaagt gatgctgtat tgagaaaaat gtgggtaaaa aagggcgagg
    13261 ttttcttaca aaagacattg gaaaaagaaa atatttttaa gttagaaaaa atggatgtag
    13321 aggatagaaa aaaattttta gatataacaa aacagtttat tagagatgct agaaaatttg
    13381 atgatgattt acctataggt gatattatgc aagctatgag gaatgtatgg atttcaaatg
    13441 cattacaatt attatttggt aaagaagtat attattcaaa agctaacttt gcatatagta
    13501 tgttatatcc atatactgat aattatttag acaatacaaa tatagataaa aatgataaga
    13561 ttttatttaa taactggtta gaaaaaaggc tcctgggaga acacattaaa tctaaggatt
    13621 atcatgaaag taaagtatct caaatgatag attatattga aagtgtatac cctagagaaa
    13681 agtttacaga agtttatgaa tcgttattat taatatttaa aagtcaagta aatagtttaa
    13741 aacaacatgg taaggaaaat catttgtgta aagaagattt attatccatt tctattgaaa
    13801 aaggaggttc atccgtttta gtagatggat atttaataag tggattgatg acaaaggaag
    13861 aaatagagtt ttgtatagga tatggatttt tattacaaat atctgatgat ttacaagata
    13921 taaaagaaga tttaaaatac aaccataaaa ctattattac agagatgtca aaagagggta
    13981 ctttagataa agttgtaaat aaactaataa attttactat tgagttaata gatagtttta
    14041 aaattaataa taaaaataaa tctgtaataa ctatgataaa gaatgattgc ttaatgttaa
    14101 ttttattttc tgtagtttat aatgctgaat ttttttctgt aggatatata aaagaa
//
LOCUS       DAEMIK010000117        14152 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEMIK010000117 DAEMIK010000000
VERSION     DAEMIK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.118"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            203..319
                     /locus_tag="KPK87_002813"
     CDS             203..319
                     /locus_tag="KPK87_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBG2538946.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            483..1550
                     /locus_tag="KPK87_002814"
     CDS             483..1550
                     /locus_tag="KPK87_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2538947.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1742..2272
                     /gene="rnmV"
                     /locus_tag="KPK87_002815"
     CDS             1742..2272
                     /gene="rnmV"
                     /locus_tag="KPK87_002815"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG2538948.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2443..3312
                     /gene="rsmA"
                     /locus_tag="KPK87_002816"
     CDS             2443..3312
                     /gene="rsmA"
                     /locus_tag="KPK87_002816"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG2538949.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3378..4652
                     /locus_tag="KPK87_002817"
     CDS             3378..4652
                     /locus_tag="KPK87_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538950.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEVSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANG
                     VSQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTM
                     PYLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGS
                     TGFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4803..5918
                     /locus_tag="KPK87_002818"
     CDS             4803..5918
                     /locus_tag="KPK87_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2538951.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6104..7000
                     /locus_tag="KPK87_002819"
     CDS             6104..7000
                     /locus_tag="KPK87_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2538952.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7070..7867
                     /locus_tag="KPK87_002820"
     CDS             7070..7867
                     /locus_tag="KPK87_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538953.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7922..9274)
                     /locus_tag="KPK87_002821"
     CDS             complement(7922..9274)
                     /locus_tag="KPK87_002821"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG2538954.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9493..10371
                     /gene="purR"
                     /locus_tag="KPK87_002822"
     CDS             9493..10371
                     /gene="purR"
                     /locus_tag="KPK87_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG2538955.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10527..10805
                     /gene="spoVG"
                     /locus_tag="KPK87_002823"
     CDS             10527..10805
                     /gene="spoVG"
                     /locus_tag="KPK87_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG2538956.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11018..12397
                     /gene="glmU"
                     /locus_tag="KPK87_002824"
     CDS             11018..12397
                     /gene="glmU"
                     /locus_tag="KPK87_002824"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG2538957.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12492..13442
                     /locus_tag="KPK87_002825"
     CDS             12492..13442
                     /locus_tag="KPK87_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG2538958.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT         5714 a         1494 c         2589 g         4355 t
ORIGIN      
        1 aatattgagc gattattaaa aagatatata tatatataat ttaatttgag cagtttgtta
       61 tatattataa aaaagagaaa aataaaaggt aaattgtttc tggataactt aaaattggaa
      121 gtaagaatat ttaactagag aagaattatt taagctcagg ttagctctag gagtagctga
      181 ttttatagga gcaatagatt ttatggatga tgatataaac atgatagcaa ttagaccaga
      241 agatataagg gtagaattta atagagattt caaagaaatt atagacctca aactcagacc
      301 gtatcgatat aaaaactaaa gctagatgat atagtgttgt atataggacc atcagctgtt
      361 atggcagtat tagtgctttt aacaaatata atagtaaaac ttttgtatga attgataata
      421 aaaaaattaa aaaccaagac ataaaaatgg caacaaaaag aaattgaata agggaggtaa
      481 aaatgaaatt taagaagtta gtctctttgg gaatattatc aacactttta ttgagtgtaa
      541 taactggatg tagtaatgtt tcatcaggtt caaacttaaa gtctaataat aagacaaaag
      601 gagagttaac agtttataca gcaattgagg aagatagtat agatacatac ttagctacat
      661 ttaaagagaa atatccagat attaaattga atgtacttag attatcagta ggaggtataa
      721 cagcaagatt attagcagaa aaagataatc ctaaagcaga tgtgttatgg ggtgttgcag
      781 ctacaagttt gataatttta gatgacaaag aaatgctaga gccatacgct ccgaaaggct
      841 ttgataaggt aaatcctgca tttaaagatg ataagaaagt tccttcttgg gttggaatag
      901 atgcttggat gactggtata acagtaaata ctaaagaatt aaaagctaaa aatatatcag
      961 ctcctagttc ttatgaagat ttaataaagc cagaatataa agatcttata tctatgccta
     1021 gtccagcttc ttcaggaaca ggatatttaa cagtatcaga actcatacaa attatgggtg
     1081 aagaaaaagc atggcagtat atggataaat tgcataaaaa tattggtgta tacactcagt
     1141 ctggttcagc gccagctata ttagcagctt caggagaata tcccattggt atatcttttg
     1201 gaaatagagg tataaagtta aaaggtgaag gttatccagt agaagtagta tttccaaaag
     1261 aaggttctgg atgggagata gaatcaaatg cacttgtgaa aaaagataat ataaaagaag
     1321 catcaaaagt atttttagat tgggctataa gtaaagatgc aatgaatgag tattctaaga
     1381 attttgcagt tactacaatt gacacgggaa atccggtacc agaaggattt ccaaagaatc
     1441 cattagaaca gatgatagat aatgatttaa aatcagcagc aagaaataga gaggctatac
     1501 taaataaatg gatagaaaaa tatgatggaa agacagaaaa agagagctaa gccttacaat
     1561 aaaatttagt acaaaaagcg cctagaatat aagttttata tctaggctct tttgatatat
     1621 ttatttaaaa tgcatatttc tatataaaaa taaatagttt agtaccttga ttaaattaaa
     1681 taaaaacctt aaaataaaga gatgtaaaga aaaactaaaa acgaataaaa ggtgaattta
     1741 tatgataaaa gaaataatag tagtagaggg tagagatgac gttacagcag ttaagagagc
     1801 aatagatgca gaacttataa ctacaggtgg atttggattt ccaaaaggtg taatggagcg
     1861 tataaaagct gctaatgaaa gacgtggtgt tataatattt acagacccag attttgcagg
     1921 tgaaaaaata agaaaaaaaa tagcatcaga agttccagga tgtaaacatg catttctacc
     1981 aagagaggaa gctaaaaagg atggagatat aggtattgag aatgctactc ctcaaagtat
     2041 aatagcagct ctgaataaag ttagaactga aaatacagaa aaaagagatg aatttaagca
     2101 agttgactta attagaaatg gtcttatagg taatgaagaa gcatcactta gaagagatgc
     2161 acttggaaaa atacttggaa ttggatatgg aaatgcaaaa caatttttaa atagattgaa
     2221 taattatgga gttgaaagag aagaatttat agatgcatta aaacaaatct aaacgtaatg
     2281 tttaatgtat gaaaaatatg tatataaaat aaattgtgga attacggtta atttaagata
     2341 aattatttat aatatacacc ttattttaaa tcaatgtaaa tacatataaa taagtataat
     2401 taataaaatt aaatatcaaa ataagttaaa aaggagtaaa taatggatag actatcttca
     2461 cataatgcaa caaaggaagt agtacaaaaa tataatttta aattttcaaa gtctttaggt
     2521 caaaatttct tgatagatag taatataatt gataaaatct tatctggagc tagaatcaca
     2581 agaggagata atatcataga agtaggacct ggtataggaa ctcttactcg tgagatgggt
     2641 aaaatagcag aaaaggtagt agctatagaa atcgatagaa atctaattcc tatattgaaa
     2701 gataccttgt cagatttaga taatacagag gttgtaaatc aagatatact aaaagtagac
     2761 atacaggaat tagtaaaaga taaattaaat ggtgggccag ttaaacttgt agcaaatctt
     2821 ccatattata taactactcc aatagttatg aaatttttag aggaagacat acctgtaaca
     2881 gacatagtag tcatggttca aaaagaagtt gcagatagaa tgaatgctat tccgggtact
     2941 aaagattatg gggcattatc tatagctgtt caatactatt gtgatacaga gattgtggca
     3001 aaagctccaa gacatatgtt tataccacaa ccaaatgtag attccacagt tataggttta
     3061 catgtaagag ataaaagaaa atatgatgtt cataatgaag acatattttt taaaacagta
     3121 aaagcatcct ttggacaaag aagaaaaact cttcttaatt cgttgggagg tcttggtttt
     3181 cttaataaag atgaaataag agaagtatta aaagaagcta atatagacga aaaaagaagg
     3241 ggtgaaactc ttagtataga agagttttct gttctttcaa atataataaa tacaaaagta
     3301 tcttctaaat agaaggtact tttttttatt ttgttaacat atattgttta gagaaactgt
     3361 gttaaggggg gattgcagtg ggttctaaaa gaaagtttaa aagagactat ataatactag
     3421 aagctaaaga cataaacttt agatacaaag aacgagtact gccaaaagct tttgcaaaag
     3481 ttgaagtaaa tgatgaaaaa gcaattattg cattatatgt agaaaatcta aaatatgtta
     3541 aagatgggta taaagcagta gcaataaaag atgaccatgg attaatagac ttaggaaata
     3601 taattttaaa tgaacaagga agaggtgaat ttgttttaga tttagaagat tctgatgatg
     3661 agataaaagg tattgcatta ttgtatgaaa gaaatatacc tttaattgga tttaagggaa
     3721 ataagcttga aaattatgag gatatattat tccaagaggt agaggaagaa gaatatgaag
     3781 aatatgaaga gttggaagaa gaagacgaag agatagagga gaatgaggaa agtgatagag
     3841 acgaagttag tgaagaagaa tatattatag aagaatatga tgaagatgag tatgaagact
     3901 attgggaaga tgatgaagat attgaagaag gagagttaaa agcaagaacg gaagtagaag
     3961 attctggtga tgaggaaata gaagagttca ataattataa ggaaatagaa aattataata
     4021 gggttaaagt acaggaagaa tatatagaca tagaatatga tgaggaagaa gaggaagaag
     4081 aggaagaaga agaagttttt gaagtaaata gagaagaaga taggtttgtt tcctcaaaac
     4141 ctactaaatc aaagaaagtt caaaaagatc agaataatat tgaaaaaata aaaagagcta
     4201 atggagtaag tcaaggagag agtggaaata aacaaaccag tacattactt atgccaagac
     4261 aaataaaaaa aggtttgaaa ttatttaaag aagtaaaacc ttttgtaaca gattatatag
     4321 ataagacaag atggtggaaa atagagataa atcctacaac attgtgtgga tatacaatgc
     4381 cttatttagg atatttaaac gtgttgaatt acaccatgta tagtgatgca gttttacatt
     4441 catataaata cagacactat ttatttggtg ttcagtatga tgaatataat aagagaaaac
     4501 attatattta tgctatccca ggaaatagaa atgaacagcc agacaatgga agcacaggat
     4561 ttttaagata tcaagcttgt gataatagaa caaatagttt aggatattgg ctatgtttta
     4621 tagattctag acaaagaaat atagttaggt aaaaacaata aaaatgaaaa aatttagact
     4681 caattcttat tttactgaaa aaattaatga aatgtagtac aaaactttaa aaactattga
     4741 taatatttaa atattacact acaatatatc ttgaaatacc tattatatta tggaggaaaa
     4801 ttatgataaa cgagcaaaga attttaaatg agtttcttga attagtacag atagatagtt
     4861 tatctttaaa agaggggaat gttgctaaag tattagttaa aaaattagaa gaaataggtt
     4921 gttcagtagt aatagacaat gctggtgaaa aagcaaatgg agaaactggt aatataatag
     4981 ctacattaaa aggaaataaa gaaggaaaga aaatattatt tagttcacat atggatacag
     5041 taactccagg tataggggta aaacctatag tggatgaagc aaatggaata ataaaaagtg
     5101 atggtactac aatattaggt tctgatgata aagcaggtat agcagctata ttagaaggct
     5161 taagatatat aaatgaaaat aatatagaac atactgatat acaagttgta ttctctatat
     5221 gtgaggaatg tggattagtt ggagctaaaa atttagacta tggaaaaata gattctgaat
     5281 atgcatttat attagatagt ggtggttctc caggtgaaat aatagtaaaa gctcctgcac
     5341 aagatgtaat aaatgttaag atattaggaa agactgctca tgcaggtctt gaacctgaag
     5401 ctggaataag tgctataatg gtagctgcca gagcaataga aaatatgaat ttacttagaa
     5461 tagacgaaga aacaactgct aacataggaa ttataaacgg tggaacagct acaaatatag
     5521 taacaggaga agttaatata attgcagaag ccagaagtct aaaagaaaat aaattagatg
     5581 ttcaaactaa acacatggta gagacttttg aaaaagctgc aaaagatttt ggagctcaaa
     5641 ttgagataga tgtcaataga gcatatacac caatagatgt tagtgaggat tctgaaataa
     5701 taaaacttgc aaagaaggct ttttcaaact taggtataga aggtcatact gaatctacag
     5761 gaggaggtag tgatacaaat atattaagta agaatggtat cgaagctata actttaggaa
     5821 ttggtatgaa aaatgctcat acactaagtg aacatatagc tataaaagac ctatatgatt
     5881 ctgctaaaat ggtagtggag ataataaagg aagcataaat ttaaataata gtgtataata
     5941 tatatatcgc ctagcttaag ctaggtgatt tttcatttta tagaagattg ataatctcta
     6001 ttgaaagcaa agagtataca tttgcatata agagagtgtt taatttatgt tcaaatgtag
     6061 gttcttaata atatttagat tgtttcataa gggaggaata aatatggaaa caaggtatga
     6121 agaagcaaat aaaataacaa tacaatcaat actttggaat atagtattaa caataattaa
     6181 ggttatagct ggtgttattg gtaattctag cgctatgatt gcggatggat tacattcagc
     6241 atcagatatt ataagttcta taggagtact aataggcaat tatgtatctt ctagacctgg
     6301 agatagagag cataattatg gtcatgagaa agcagagaca ttagtatcat ttgtgttatc
     6361 aatattgctt attttcgttt ctataacaat aggtatagag gcaattaaat ctttatttaa
     6421 cttagatgca ttaagtgttc catcaatatt accattagta gtatcagtta tttcaatcct
     6481 gataaaagag tatcaatata gaataacaat aaaagttgct aaaaagataa attcaccagc
     6541 cctaaaagct gatgcttggc atcataggtc agatgcttta tcttcagtag ctgcttttat
     6601 aggtataggt ggttctattt tagggtttaa accattagac ccaattgcgt cagttgtagt
     6661 ggctatattt gtagctaaag taggaattag tatacttata agttctgtta atgaattgat
     6721 ggatgtttca gttgatgaag aagagataaa agaattaaaa tttattgtag cagatacaga
     6781 aggtgttaaa aatcttggtg atataaagac tagaaaacat ggagctatgg catatgtaga
     6841 tttgactata tgtgtggatg aaaatttgac agttaagcaa ggtcatgata ttgctaccaa
     6901 gcttgaaaaa catataataa aacatatgga atttgtaaaa ggaataacag ttcacgttga
     6961 accatgcact aattgccaag gaaataagtg taataattaa ttaaaactta aaaaaaagtg
     7021 taaaaagtta tataattgtt ttatgattat aaactctagg ggtggttaaa tgagcaagaa
     7081 aagaaaacta aagaaaaaag ctgcaatatt aataggagta gttgttttct ttataatata
     7141 ctttatatcc tttagaatag gaataaatat ggaaaaggat aaaagtgaag tgccaaagaa
     7201 ggtagaaaaa gaagaagaag ataataggtc tttaaggact caaataaaag agaagaagaa
     7261 ggtttatata tcagataaaa atatagaaaa cataaaaatt gaagaagata tgtgggatga
     7321 aataaagtta tttttctcag attttaagaa agtaagaaat actacttctt cttacgaagc
     7381 agtttatgaa ggttattctg atgatggagt taaattttct actgacctta acttttttag
     7441 agtatatact gtaaataaag aagaatatta taaagtacca gttagttcta aggatgcttt
     7501 taagaaactt ttagatgaga gtatatatac ttcgtttgac cttttaagtc attataaaga
     7561 ttggaaagag gttactataa ctcatggaaa agaaattaaa aaagttcata gatggaaatt
     7621 tgatgattta gcacataaga tgatttcaaa gagggttgta ggaaaagttc aacctgagaa
     7681 aagtaaggaa agaagtgact ataattttac tataaatata aaaggtgata attttgatgt
     7741 aaaagttgat acaatgggaa aagattatgt aaaagttact tctaagaaaa atgtatctta
     7801 ctatgaggtt catacaagtt tatttgatta ccttaaaaat gagatatttg aaattggaaa
     7861 gaaataagac tttaagaagg ctatcaaatt agatatttga tagcctaaat tattttatat
     7921 attatttttc aaccaacaag tctgcaacct tataaattgg accagcacca gcagttatta
     7981 ctaaatcatt atccttaaca ttttcacgta agtattttac tatatcttca aattctttta
     8041 tatagatggc atctacattg ttttgatata atttctcaac caagtcctta gaatgtatat
     8101 ctcctgggtc tttttcccta gcagcatata tatctgttat gataacttta tcagcagcat
     8161 agaatgcctc tgaaaattca cctaaaagag attttgttct agtataagta tgtggctgga
     8221 agatacacca taaagttgat ttttttaatt ttttggcagc agctaaagtt gcttttaact
     8281 cagttggatg atgtgcataa tcatctacta ctaaagcatt cttataatac cctttagttt
     8341 caaatcttct tccgacacct ttataaattt ttatattttt tcttatagtc tctaagtcaa
     8401 taccagaaac aagagctgcc attatggcag aagaagcatt gtatatgtta tgaagaccat
     8461 atacagaaag ttcaaattct cctaaatctc ttccttcata ttctattcta aatattccat
     8521 gaccactatt gtcaaagtga atatctttta ttacagcatc attgtcagaa tctctgccgt
     8581 attttataat agtagcttta acatcataca atatgtcatc aatattttca tcatcgccat
     8641 ttattatgaa atagccttct gggggtaata attttccaaa tttgttaaaa gaagccttga
     8701 tttcatctat tccagagaaa taatcaagat ggtcttcttc aacatttaaa actatagata
     8761 tctttgggtt aaaattaagg aaactatcta catattcaca tgcttcagtt ataaagtggt
     8821 tggaattacc tatcttaaca ttaccaccta tcatacttaa gttaccacca actagtatag
     8881 ttgggtctaa gtctgcatat tcaaagattg tagataacat agatgttgta gaagtttttc
     8941 catgtgttcc agaaactgct atagaatttt tatattccct catgatttgg cctaaaaaag
     9001 ttgctctgtt cattaccaat ttattttttt ctttagcagc cacaagttct tcattgtctg
     9061 gatgaactgc tgctgtataa acaaccatat ttacatcatc tgatatatta tcttttttct
     9121 gaccaatata aatagttgcc ccttggtccc taagtttgtc taataggtat gattcgtttg
     9181 aatccgaacc tgaaacttga taacctttat tgatacatat ttcagctaat gcactcatgc
     9241 tgataccgcc aattcctata aagtgtatat tcataataaa accacctttc tttttaatta
     9301 agtcctacaa tatgaactat ttctgtggta taataatata atgttcaata ttttataatt
     9361 aatatgcata taatattata tgtattttat ttttgaatat aataaaacat aagtaatatt
     9421 ataccacaat aagaattgtt atgcattagt tagtttaacg ttatataaag gtacttgtag
     9481 gaggaaataa taatgaaatt taaaagaaca gagagaattg gagcaatagt taaaatactg
     9541 tctgataatc caaataaaat atatacttta agttatttta caaatcaatt taatgcagca
     9601 aaatcaacaa taagtgaaga tttattagtt gtaaaaaatg tatttgagaa gctacattta
     9661 ggtaaagtta taactatttc aggagcagca ggaggagtaa aatatatacc taaaacttct
     9721 atagcagaaa atgcagagtt cttaatggaa ttatgcgaga gaatatgtga caagtctaga
     9781 atactatctg gtggtttttt atacttgatt gacctaattt acgaccctac aatagctgct
     9841 aagataggaa agatattcgc atcaaatatt gagtatgtag atgcagatta tgttgtgaca
     9901 atggaaacaa aaggtatacc tatggcactt atgactgcaa aagctatgaa tttacctcta
     9961 gttataataa gaaaagatat aaaggtatca gaaggtccta cacttagcat gacatatgtg
    10021 agtggaaaca gttctaaagt tgaaagtatg agtttgccaa gaaaagctct taaaccagat
    10081 agtaaagtta taattataga tgatttcatg aggggtggag gtactataaa aggaatggta
    10141 gaccttatga atgaatttgg tgcagaggtt ataggtactg gagtattcat atctacaacg
    10201 aatccttcag aaaaaatggt aaaagattat atttcattga ttcaattgga tgttaatgga
    10261 gataaaatag tagtagaacc aaacttaaag acttttaaag atgaatatag aaatgaagat
    10321 ttagacgaag aagaagattc agaatttgaa tttgagatag atgaagatta ataaaaaaaa
    10381 tatttagaaa aatatgtaaa aataaaaaga ggatatccct agttgttcat agaattattt
    10441 aaaaaacagt ttatattaat ttattccagc gataataatt ttaatataaa ttaatttctg
    10501 tcaaaatcaa agggggatat tttggtatga agataactga cgtaagagta agaaaattaa
    10561 cagaagaagg taagatgaaa tgtatagttt caataacttt cgataatttg tttgtagtgc
    10621 atgatataaa agttatagaa ggccataatg ggctatttat agcaatgcct agcagaaaag
    10681 taggcgaagg aaatttcaga gatatagctc atccaataaa tgcagaaatg agacaagttt
    10741 tagaagatgc agttttacaa gcgtatcatg aagcattagt tcaatgggaa gtagctgctg
    10801 aataataata aatatataaa atggagagag cccgaacatt taggctcttt ttaaatgtag
    10861 aatttctatt aaaatatgta ctttttagta aaaaagtgct atattattag tgataatgta
    10921 tatgttgtat acaaattgtt agataaatgt attataatgt atattgatta agagaataat
    10981 acgaataaaa ttaaattaga ttgaatggag tgtatcaatg aactttaaag ctataatcct
    11041 tgctgctggc aaaggaacaa gaatgaagtc taaataccca aaagttatac ataaagtatg
    11101 tggaaaagaa atggttaacc atattataga tgtttcaaaa aaatcagggg taaaagatac
    11161 tgtcgtgatt ttaggacatg aagctgatgt agttaaagaa aagcttgcag aagaaattat
    11221 aatagccatg caaacagaac aacttgggac aggacatgct gtaaagatgg ctaaagaata
    11281 tattaatgat gaagatacaa tagttgtact ttgtggagat actccactta taaaagaaga
    11341 gacactaaag agactatttg aatatcatat agaaaataaa tatcatgcta cagttcttac
    11401 aacaagagtt ggaaatccaa caggttatgg tagaataata agagataaga agggtgacct
    11461 tcttaaaata gtggaacaaa aagatgcaaa ttcagaagaa aagatgataa gtgagataaa
    11521 ctctggaatt tattgtttta atggaaagag tcttagagaa gcactagatt tgctaaataa
    11581 taacaattca caaggtgaat attatttgac tgatactgct aaaataatga gagataaagg
    11641 attaaaagtt ggtgcatttg caggttcaac tattgaagaa cttatgggcg ttaactcaag
    11701 agtagaatta tcaaaggcag aagaaattat gagacgaaga ataaatgaat ctcatatggt
    11761 aaatggagta actataatag acactaactc tacctatata gaatctgatg ttatgatagg
    11821 taatgatact ataatatatc caggtgttat gttacaagga aaaacaagga taggttcaga
    11881 ttgtataatt ggaatgaatt catctataac aaattcagaa ataggtgatg gtacagaaat
    11941 aaaaaattca actataattg atagtaaggt tggagaaaat tctactgttg ggccatatgc
    12001 atacttaaga ccaaagagtg acttaggaaa taatgtaaaa ataggcgatt ttgtagaagt
    12061 aaaaaatgct attatagaag atggctcaaa agcatctcac ctttcttata taggagatgc
    12121 acatgtcggt aaaaatgtaa atattggatg tggagtagta tttgtaaact atgatggtaa
    12181 aaataaattt aaatcaatag taaaagacaa tgcgtttata ggttcaaatt ctaatttagt
    12241 tgcaccagta gttgtagaag aaaaaggata tatagcaaca ggttcaacaa taacacatga
    12301 tgtcccagat ggtgctttgg caatagcaag agaaagacaa gttatcaaag aaggatgggt
    12361 agaaaaaaag aatcaaaaag acgaccaaag taagtaatta tgtagtttat tattttggct
    12421 aaaaaattta gctcgaaatt attataaatt atattcatta gtattttaaa attttcagga
    12481 gggttacata aatgaatact agcggaagcg agattaaaat tattgcaggt aattcatcga
    12541 aagagttagc acaaaaaata gctgactata taggtgtatc agtactagat tgtgaagtag
    12601 gtacatttag tgatggtgaa atatgtgtaa acatgaatga gacagtaaga ggttgtgatg
    12661 tgtttgtagt gcaatctact aacagcccag taaatgataa cttaatggag ttattaatct
    12721 taattgatgc gttaaagaga gcatcagcag gtagaataac agcagttatt ccttactatg
    12781 gatatgcaag acaagacaga aaggctaagg caagagatcc aatcactgct aaattagttg
    12841 caaatttaat aactgctgct ggagcagata gagtattaac aatggattta catgcagctc
    12901 aaatacaagg atattttgat ataccattag accacttact aggtggaaca atattagcaa
    12961 attattttaa tgaaaagaaa atagaagatt tagtagtagt atctcctgac ttaggaagtg
    13021 ttactagatc aagaaaattt gctaatactt taaatggaga agttccaata gctataatag
    13081 ataaaagaag accgaaagct aatgtatgtg aagttatgaa cttaataggt gatgttaaag
    13141 gtaaaaatgt tatactatta gacgatatga tagatacagc aggaacaata gttaatgctg
    13201 caaatgcact taaagagttt ggagcaaaag atgtttatgc ttgttgtact catggtgtgt
    13261 tatcaggtcc agctattgaa agaattgcta attcagaaat aagtgagtta atagttcttg
    13321 atactataca acttcctgaa gagaaaagaa tagataagat aaagataaaa acagtagctc
    13381 ctttatttgg agatgctata agaatgatat tctctaatga atcagtaagt aaattattct
    13441 aatttattgt atttatgaga gttgtttaaa aggatataaa ccttttgagc aactcttttt
    13501 tgattttata cttatggaat gttatttttt tcttgttaga aaggtatata ttattgtaaa
    13561 aagagattat agtttaaaat tatgtatatt taagtaaaat aaacctaata aagggtatat
    13621 tattcaaaat attatgaata taaaaaatat tacaaaaaag tattgaaaaa attcagatta
    13681 tgtagtatta tgtaaatata aataaagaaa gaggtgtttc caatgggaag gttagaagat
    13741 gaacccctaa taattaaatg attcagtcac aatctaaagt gaatataaaa atctattgaa
    13801 agtttattta taagaattga aaatagtatc tattctatct ttagtgtttg tgagagggtt
    13861 ctggattata taagattact gtaaagagta attttgcaca ataagcatat ctgtaaatga
    13921 agagtttcgt ttatataatg attgtggctg aatcataact ataatagata gaatgtttat
    13981 aatagatgat tttataaaat actatattgt aatagctttt agctttcaaa taaatattag
    14041 taaaggatgg aaatgacaat gataaaacta aatgataatt tataaatatc tccttacgat
    14101 atataataat tgtagggaga tgtttctttt tttgtatagc ataatttttc ta
//
LOCUS       DAEMIK010000118        13980 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEMIK010000118 DAEMIK010000000
VERSION     DAEMIK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.119"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            392..1795
                     /locus_tag="KPK87_002826"
     CDS             392..1795
                     /locus_tag="KPK87_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2538959.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2196..3218)
                     /locus_tag="KPK87_002827"
     CDS             complement(2196..3218)
                     /locus_tag="KPK87_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2538960.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPKLI
                     ERGSTL"
     gene            complement(3253..4851)
                     /locus_tag="KPK87_002828"
     CDS             complement(3253..4851)
                     /locus_tag="KPK87_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG2538961.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVVLKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4893..6158)
                     /locus_tag="KPK87_002829"
     CDS             complement(4893..6158)
                     /locus_tag="KPK87_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2538962.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6302..7714)
                     /locus_tag="KPK87_002830"
                     /pseudo
     CDS             complement(6302..7714)
                     /locus_tag="KPK87_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
     gene            complement(7752..8759)
                     /locus_tag="KPK87_002831"
     CDS             complement(7752..8759)
                     /locus_tag="KPK87_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2538963.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(8956..10278)
                     /locus_tag="KPK87_002832"
     CDS             complement(8956..10278)
                     /locus_tag="KPK87_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538964.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10636..12276)
                     /locus_tag="KPK87_002833"
     CDS             complement(10636..12276)
                     /locus_tag="KPK87_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG2538965.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12381..13037)
                     /locus_tag="KPK87_002834"
     CDS             complement(12381..13037)
                     /locus_tag="KPK87_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538966.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13063..13764)
                     /locus_tag="KPK87_002835"
     CDS             complement(13063..13764)
                     /locus_tag="KPK87_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG2538967.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4753 a         2627 c         1667 g         4933 t
ORIGIN      
        1 ataattatat tctaatatac tacctactac tttgcgctac aacaatttct ctatttatat
       61 gatttgtaaa atatagtaca acatttttta tcattttcat ttcaactaaa tttgtaatat
      121 tttattcaat acttaataaa tacttaatca atacatgctt tataatactt tactcaatat
      181 ataatcaata cataatttat aatttgtaat actttattca atacataagt agaaatctct
      241 atatacataa attcattttt tcatacatct tacaaattta tttttattta gataacatgt
      301 atattttttc catgaaaaat aaataataaa aaatagtaaa tatggaaaat ataaaattga
      361 ttaacaataa ttaattacct gaggtgtgaa tatgaaaatg ctaaacaact tttttaagaa
      421 aatcaacaat ccagctcctg tctttgcaca gacaaaaatg gaaagagata atcaaacaga
      481 tgattcactt tctacaaaac caaagactac atttagagac gttgctggat tagacgaagt
      541 aaaagaagaa ctatttgaaa tagtcgattt tatgaagtca ccacaaaaat atcaaaaaat
      601 gggtgcaaaa atacctaaag gagtattatt ttatggtcct ccaggaacag gtaaaaccct
      661 acttgcatct gctgtagctg gtgaaacaaa ctcatcattt ttcaatgtta ctggttcaga
      721 gttcgttgaa aaatatgtag gtgtgggagc aaaaagagtt agaactctat ttgaaaaagc
      781 tagaaaagaa gctccaagta taatatttat agacgaaata gatgctgtag gagcaaagag
      841 acatcttgaa agtaacaacg aaaaagacca aacactaaac caacttttag ttgaaatgga
      901 tggatttaat aaagattcca atgtattaat aataggtgct accaatagac ttgacttatt
      961 agatgaagct ttacttagac caggaagatt tgacagacac attcatatag gtgcaccaaa
     1021 ctatcacact agatttgaaa ttctaaaggt acatacagat gacaaaccta tagacaaatc
     1081 tgtaaatcta gaacttttag ctaaaaaaac tcatggtttc aatggggctc atctttcaaa
     1141 tatagcaaat gaagcagcta tatttgcagt tagagatgac agtgagtgta ttacatcaga
     1201 acattttgac aaggcacttg aaagagttat tgcagggcta gaatctaaaa actctgcatt
     1261 ggttgaaaaa gagaaaaaaa tagtagcata tcatgaagct ggacatgctc ttgtaagtga
     1321 tatagtagga atttgtccta tacaaaaaat atctatagtt cctagaggac aagcattagg
     1381 ttatgttctt caattacctg atgaagatag atatatttat actaaggatg agttgattgg
     1441 aaaaataaaa atattacttg ctggaaaggc ttcagaagaa ttaatattta atcataaatc
     1501 tactggtgca aaggacgact taaagaaagt cactgaaata gctaaccaaa tggtttgtga
     1561 atatggtatg agtaacttag gatttatgac tattgatgga aatgataaga ctttcttatg
     1621 tgataaggtt caaaaggaag caaatcgtat agttgaaatt tgttataaag agactttaga
     1681 gatgttaaag gataatcttg aagatttaca ttctgtttct aaattcttat ttgaaaaaga
     1741 aactatgact catgaagagt taaaagattt gataggaaaa gaagctgtaa actaatatag
     1801 ttttaaaaat ttgaggctgt ctcaaaatag agataagcct ctttttattg ttaaagttat
     1861 aaaaatacat ttgattttat acaaaaaagt gcctcataaa ctaaaaattt cattttgaga
     1921 cacttacata ttttaattac atattttaat tttgtctatt tacagaatat tatatctaca
     1981 taaaactaaa ctgatgtaaa cttaactacc tccagtaatg tttgaagtgg tataaaagct
     2041 agtagcctag tttctacact tataatttat attttacata actaaattta gtagattttt
     2101 atactcacaa tttagaacga ttgaaaactt aaaaatttct tatcagatat ctacaactta
     2161 attaggtatc taccacttaa ttaaatatct accacttaaa gtgtagaacc tctttcaatc
     2221 aatttaggtt ttaacatttc aacaactgac tctggatttt cttcatttaa taaggactta
     2281 agtatgttaa ttgttttttc aatcaacaaa tctagtggct gtctaatcgt cgttaaatta
     2341 taactcttcc atgaagccat ttgaatatca tcatatccta tcactgataa atcctctggc
     2401 accctcaact tgtactcaaa ccttgcagca tccattacac caatagctat caagtcactt
     2461 gtgcaaaaaa ttgctgttgg aggatttgat aatgctaaca ttctcttacc tgcttcatat
     2521 ccagaatcat aatcatagtt tgcagattct tctttaaggt tatgaatccc actttgtctt
     2581 aacttagaat aaaatccaat tttcttttcc atttcttcac ttgtttctct cgtaaattga
     2641 atatatccta tattcttatg acctttctta agcagatatt cagcagccat gcccccacct
     2701 tcaattgggt ctacatagac tgtacttatt tttataccag gtataaaacg attaaaaaga
     2761 atcacaggaa tattgttttc ttcacaagtc tcagccatca ccttattcat ggccgaagct
     2821 gtaataatca cagcttctac ttgaaactga attacttttg aaattatact atctatactt
     2881 tgttgtctag gaactttaaa aacgagacac tgtttaccta tctcttgaat tttttctaca
     2941 aataaattga taattctatt gtaaaatgga cctagactat caccaactat caatcctata
     3001 acattagttt ttccactaat cattccccgt gctaccaaat taggaacata tcctatttcg
     3061 tttgctacac gaaaaattct ttctcttact tcaggtttta cgccctttcc atttgcacta
     3121 ccaaatgctc ttgaaacaga tgattgagat actcctgcca atcttgcaac atcttttgag
     3181 gtagccgatt tattataatg aactttcttt tgattcattt agtatttctc cttaaatatc
     3241 tagtatttct tcttaaaatt gtatcaaaaa taatataata tattggataa taaaaaaagc
     3301 taatacaatt agttcttgtt tacctaaaga aaatggcact atagatactc catctacttt
     3361 atccattcct tgagaaataa ggtgatttct cataccaaat gcagtgtatt ttttaatatc
     3421 atctgcatta tgatttttct ttaaaacgac actaaaaata tatccaaata tcaatgttat
     3481 tgtagcacct attgctggat tccacaacca actcacatta tatttattag aaataaaaaa
     3541 tcctaaaact aaccctacaa taaaaccaat agatactcct ttatcattag ctttatatgt
     3601 aaacattgct agtacaaatg cacctgtagc tggtccagca aagtatgaga tatagttacc
     3661 aacaaggtca agaattgatt tgatgcttcc tccaaatccc ataataataa aacctgtcat
     3721 aacaactcct ataactacag ttataatcat tgtaactttt aatgatgatt ctttgttatc
     3781 ttttttgaaa taaaactcat aaatatcctt cgtaaaaaca gttgtcatac tgtttaatat
     3841 agagtcgata ctagacattg ctgctgcaaa cacacctgca ataataattc caatggctcc
     3901 tactggaagt tcatttaaaa taaaagtaat cattatttca tttgaagttt caaatgtcct
     3961 tcccccataa aaaacaaata aaattactcc taccaatagc ataaagtagt aaactaaatt
     4021 cataacaaat gcactaacta caaaagaatt ttttgcacat tttaaagatt tagatgttaa
     4081 aactctttgt acttgagatt ggtcaaaaca aaagtatctt atccacatta cagttccacc
     4141 aattaaagta gcccaaaaag tatttgtcac tgaaatatcc ctactgaaat caaatgtatt
     4201 taattttcca ctctgttgtg caatttgtaa tgtatcaaag aaacttagat tcattccttt
     4261 taaggctaca ataattacta taaatacagc acttattact actatcattt gaatactatc
     4321 agtccatata actgccttaa tcccacctaa tagagtgtat ataattgata caagtacaac
     4381 tattgggact aaagcataaa gtgaccagcc agtaatcatt tgaataataa gcactggaat
     4441 atataccata gaactagctt gaatcaatga attaataaaa aattgaataa tagttaatcc
     4501 tcttgaataa tttcctaaac gaagtcccat atattcatat atagaggtta tttttaattt
     4561 atagataact ggggtggtaa tgctcagtgc aaaaaatatt gctaaaggta ctgtaatatt
     4621 aaccattact ggatacattc catctacata tgcccatcca ggaccaccta taaagccatt
     4681 tgcactaatc atagtagctg caactgataa agcaattgga atccatggca ttgacctacc
     4741 tgccaagaaa taatcttctt gatttttatt atcttttcct gaataataac caataactaa
     4801 cattaatagc aaatatccta ttataataca tacatcaatt gatgaaatca taagccaccc
     4861 ctttctaagt aacaaaataa attcctagta ttttattttt tattatttaa cgtctctggt
     4921 aagaataacc atacaatagc acctgctaca aagactgcga ttgatatgta aaagatagtc
     4981 atcataccta aagtaccaga agctgtacca gctatagcag gtattgcaaa cgtagatata
     5041 atccttcctc cattaaatga cataccagat gacaacgaac gatatttact tgggaacaac
     5101 tctgtgtagt atgctcccca tgctccagaa tatccaacag caacacctag taatactgat
     5161 atcatataga atgttgttgt attatctcca cctaattttg tatatataaa aacaaatact
     5221 gctgctaatg ccatacctat aaaaggtatt tttcttccaa atttatctgc aattgaaccc
     5281 cagaataaat atcctactaa ttgaccaatt ccttgtaaac tatatataga cccagccact
     5341 gctgctgaca ttcctattcc tgtcactagg tatgtagttg cattatttga aaatgctgaa
     5401 tatgctgtaa aatttgcacc tgataaaaga atcgttccca atccaatcca taagtatttt
     5461 cccttataca tacttatata gctaatcttt tcaccagttt cttttccctt tttaactaat
     5521 tcctttgtat actccaaatc atctggcaca aagaagattg ctaagatagc tccaactata
     5581 ggtggtataa tcgcagtata catcaatatt tgccaatttg catcacctaa aacgccatat
     5641 actttcccac caaaaatcat tgctaatgaa aataaagcag ttaatatagc agctaactta
     5701 ccacgatatt tacttggaaa catatctgca actaaaggca tagctgttcc aaatacgcct
     5761 cccaacacta ctcctgtaca caaacgtgct attttccata acataatatt tgtaggagat
     5821 aacattggta taaatgttgt caatccaatc agtaaagcaa cgctaattaa agtcctcttc
     5881 ttcccaattt ttgtagaaat caccccaaaa ataaatgtac ctggaattgt accaaaagac
     5941 ataactgcaa atatttcttc tgtttgtttt aatgaaaaac caaatccccc atctgctaaa
     6001 gcataaatca ttgttggttt aataaaagta cccaccataa aattgtagca ataaaaaaag
     6061 tatattaaag caattgaaaa aaatgcaatt cctcttttta ggtttgaata gtcttttcct
     6121 aatatgatat cattttctat ttcaaccgtt gttttcatat ttttataatt ccccctttta
     6181 aatttagtca taattttgct aaaagtttaa acatctattt ttcatataca ttccccactt
     6241 aaattttata aagcaatact tccctatata cattttaaaa ctatttatac tctaaacagc
     6301 tttattcttc tactcccata tacatttgtt ttaatacatc cataccatca aaagttatcc
     6361 actcctcctt tactttacct tgtagtatat gataatgatt gattcccatt acttctatat
     6421 gctttccaga aggctgtcca aaaaatccta taccttcatt tattccacgt aatctccatc
     6481 ttacagatac atcataacct tcattttttt ctctttgatt acaagttact ctttccactt
     6541 cataacttcc atttggaatt gatgcaagta aacttacaat cattccttga atctcatcat
     6601 aaccatttaa atctttatca catataaaat gtactactgc attatcatga tagtattttt
     6661 taacttcatt tatatattta taattataaa tacggcttgt catttcaagc atcatttcgc
     6721 caacagaatc atcttttgct tgatacttag ttggcataaa ttgaccatcc atagattcac
     6781 agataccata agttgattgt aaactaagca ctttagattc acttgccttt gccatactct
     6841 ttgctacttc ctgagggttt aatcctaatt gagtaacaat ccataaatta tctctaacca
     6901 accattcttc gtggattcta ttgttagttg tggcacaatc aattacagtt ctaaagttta
     6961 tttttctacc tgttgctggt ccaaaattac tatcacctaa gttagttgct gtagacaaaa
     7021 cacggtgaga agataaaaaa ccatcagcac caaagttaga ccatattaca ttttgaccaa
     7081 ttaatcttct atcaggaaac gcatgtaatg tttgaagagt attggaaata acatctttaa
     7141 taccaactaa atttgaagag ccacaatgca ttgtaacatt attgtgatat gtatcataaa
     7201 taacacctat acctttttct tcccagatat tatgtgtaat cttcataata taatctacaa
     7261 aatctctata cttagaatca aatccaggta tttcctgttc cctttttgtc tgttttgaaa
     7321 aatcattgta atccattact ccaacaggcg ttacttctaa tgaatctcca tagtaaattt
     7381 ttgtagatga catattatct tcccccttaa atataacttt tttatttttt tcagtaagag
     7441 tatttgtatt taatttatct gtatccactg tatctatgtt aactttattt atgtcggatt
     7501 tatttgtgtt tgttttatct atattccctt tatctatatt tcttttatat gtgttcgttt
     7561 tattctcatt tgtttgattt tcatttatat tactaaaatt agtttgattc attttagctt
     7621 tgttttcttt aaccgtacat gattcaagcc cacttgtaga aacttctttt tcttactctt
     7681 tacttgttgc attatttttg ttggtagatg ccactttata tccccctttg ttttagaata
     7741 cgtatgcata actaagcatt aaaaatactt ttagttctct caagaatatc aagaccttgt
     7801 tgtttcaacc aataaggaat atcaattaac acccagtttt cttgaagttt atcgccctta
     7861 cgataataaa catcaacaac ttgcatatct gctttaacct ctccacctgg taatcctaaa
     7921 aaacctccac atggtacatt agtcagatta ggccatccaa agaaacaagc aaaattacct
     7981 tctgcaaatc tacatacatg cccattgaaa gtctttcctt ttaaattatt tctaaatggt
     8041 agctggtgtt gctcttgata tctaggtata gtataagaag caccaattcc acctggacca
     8101 taccaaatca tatctttagc ccaagatttt tcaagtattt ctggtgggca tcccatagca
     8161 ccactttcat ttaattcaga taaatcatct accattttat ttactaatgc taaagttttt
     8221 actccttcaa cttcatctgc atcttcaaaa agtaatccat tatggtcaat tggccctgga
     8281 tatacaaaat atgaacctgt ttgtggtggc aatggattaa ctcctgcttg aatcataaac
     8341 ccaattatat ctacaaataa tccagtttta gcaattttac catcttgaac acagttaaat
     8401 tctgcatatc ttaaactaat cattttacga gtatgtttaa tgccaagcca atcgttatca
     8461 aaaagtccca taaaatgccc catactcatt acccattcac tattgtcaat ctcatttgtt
     8521 cctgcaatga aaatatcttg tcgtctttgc atattactca atgatttttt taaaggcttc
     8581 caaaatacat ctgctacatt ttcagtgcct tcttgttcac gaaatggata cacacctttc
     8641 cagttataat ctgatttcat atgttgcttt aagacatttt caacctcatc cccagtagca
     8701 ttttctaatg cttcaaaata atttcttaca actttttttg aatcctgata ttttcccata
     8761 agtatctcct cccagattta tttaagttat aaattaactt tctcatttta tgcatacgat
     8821 ttccttttta atgacttttt agctgtttta agtttatttt atgaatacat atgcattttt
     8881 ctaaatatta acatatatct aaaaaattgt aaagaaaatg ttatcataaa aatttaattt
     8941 ttgacttata tctatttaaa acaacagaaa aggtttaaaa cgtaatattc tactgggtct
     9001 accatacttg ctcataagct gttcactcac tacctcacaa taaccagaat cacacaatct
     9061 aagtataatc ctgtccatag ttctaatact tacaccacat atagtagcta attcctttga
     9121 tgtaaattca gtttttccac tatttattat tttattgaat actttatata cagtattagc
     9181 acttagccct gtctctgtag caatttgata aaaacgttca tcaaaactat ttttctcttt
     9241 gacagtagga tttggcttta gtggtcccat aatttctcca ttttcaaaaa ctacatatgc
     9301 tgcatcattt ttttgacttt gagccatctt aatactctca taagcgttaa atttagcttc
     9361 atttgctgtt tttccataac caattcctat ataagtgttt ataactgaaa cttcacgaat
     9421 catatccaac aaataaatat ctctgtattt ctttgtttct aactcaagta tttttttagt
     9481 tgtaaaaatc ataaattcac ttctactctg ctctaccaaa acaccatcta tacggctatt
     9541 gaaatggtat agtttatcaa gaatcttaat cctttgtgaa agataagcgt actcatcttc
     9601 cttcattagt gaatattctg aaggcatgtt cagtttaata gcaagaacaa taatctgatt
     9661 ctcattgttt ttctttgcaa tataacgcat ctgaatgttt tttatacttt gcaccattac
     9721 gtcctcagaa ggcaaggcta aaactactgg aatattatct tttttcatta tatgataggc
     9781 ctcttcaaga cctgtaatac aacaacatac atcattattt tgataattat atttatgaaa
     9841 ttcaagcaca aactttatat agtcctcttc taaatacttt tgctctgcaa aaaacatatg
     9901 ttcatcctta aattcaactc caatttcttt atacaatctc ttcatcatag ccttatcata
     9961 agtatctata ctaagtctac taatatcgaa tttaagcaaa tatgtaattt caagcaacac
    10021 tctgtacaat gttgtttcat gtctaggaat ataatcagac aatacaaaat ctttttgtct
    10081 tttttcaaaa aatttaaatg gcgacttacc tgtaaaaaga actgcatcca ctctacctct
    10141 atatttttcc aataaatcag gtacttcaat atatctatca tatctaagaa aatctgcttc
    10201 aatattagga aaattctttt tacttactgc tagtatttta tcgattacca tacctggtcc
    10261 tacataaagt attttcattt cgtctctcct ttcctaatga tactattatc tcttaaaaca
    10321 ttttctaaac ctaccatgac taaagccaca ggactcctga ttcatagaat tttgaataat
    10381 caattatacc taatataaaa agttacttta agttttttaa ttacgactat ctgttgaaac
    10441 atttttgtat ttaaattttt gtatttaaat cttattcatt aatattaatt acattttaca
    10501 attaatatat tgttttttct acttaaagtt aaattcttta gaaaacacaa gtagagtaat
    10561 ctttattaat ttaacaaaaa tagccttcct taatattcaa agaaagacta tttttataaa
    10621 agttcttaat gtattctatt ttgctatgct aagaggattt ttaattacac gtccagcaca
    10681 tacatcagtt tgctttcctc ctgccaatgc aattttacca ttcaccaaca catattccac
    10741 tcccactgta tactgtttag gattactata agtcgcttta tcttctattg tatctgggtc
    10801 aaataccaca atgtcagcaa aataattttc tttaagtaga cccctttctt tataaatgcc
    10861 taagcgtttt gctgaaagat aagttatctt tttaactgct tcctcaaaag tcaacatacc
    10921 tttttctcta acatagtaag agaaaattcg tccaaaagta ccaaatgcac gaggatgagg
    10981 ctttccaaga gaaagtaaac cttctgtaga aaggtccata ctatcagaac ctatacatga
    11041 ctttggatgg caaacaacct tatctgtatc ctcttgacct tgtgtatgaa caactacttg
    11101 aattttactt ttttcttcaa tcaaaagcct aaaaaacgta tcaaatggat tttcaccatt
    11161 gattttggct acctccatta aagttttacc ctctacatat ttatttgctt cagtcattac
    11221 agaggctagt acaacaccgt tccacccacc tgctaattgg tagaaacatt gccatccagg
    11281 aatcccttcc tccaactctt ttataattct gtctctattt tcttgtactt ctaatcgttt
    11341 taccatattt tctacaccgc cttcaaaagc ccaaggaggt aaaagtgcac ccaaagatgt
    11401 cattccagct gtatatgggt aacaatcaaa agttacttcc aaaccatctg cctcagcttc
    11461 atctagcttt ttaaatacct catcaatagt tccaaaattc ttatgaccca taacttttaa
    11521 atgagaaata tgtagaggaa ctcctgcagt cttagctata taaattgctt catcaagagc
    11581 ttccatttct tcatcacctt catctctaat atgagatata tagtatccat tgtatggtat
    11641 caattcctta cataattcag ccatttcttc tttctttgtg tatgctcctg gtaaataaac
    11701 taatcctgat gaaagagcta cacatccagc ttccatagct tttctaactt ccaacttcat
    11761 atttttaagt tgctctaaag tagctggttc ctttgaaaat cccatttcat tgatacgtac
    11821 aggtccttgt gctacataag ctacaacatt gactgctgga gaattttcag ttaagtaatc
    11881 aaagtattct tgcattgtat tccaatgaag ctctaatttt gttggaattg taccaattat
    11941 cctagttcca agataatcaa ttaactcttg cttcttctct tctcttatcg gagcaactcc
    12001 tataccacag tttgcaataa catctgttgt tacacctgta tggatacggc ttcttgtgta
    12061 tttatcatac acaagagata aatcagaatg agtatggaaa tctataaaac caggtgatac
    12121 aaattttcca gttgcgtcta tagtctcttt agcctctttt tgacttaaat ctccaatagc
    12181 catgattttg tctcctacaa tccctacatc acttttatat cttgaatttc ctgtaccatc
    12241 aacgattatt ccattgacaa ttttaacatc aaacatgctt tttctccttt caagacttat
    12301 ttatattcat atttcttaat aaacaatcct attctttttt ctcaacaaat aattctagtc
    12361 tttctcctta ataagcaatc ctattctttt gttttattgt tatcaattac aaagaagacc
    12421 cttgtgataa gtatcccacc taacacaata aacaatgtta ataatccacc tggtatacca
    12481 attttttcac caaagtacat aattgctaac gctacagcaa tcgtacatcc accagctcga
    12541 atatccttac gtgcaatatc aacataaatt gctccaaata atgctggaag tatataatta
    12601 aatgaattag taacaaattc aggtaacaaa ggaattactt tatagcctac aatggtaaaa
    12661 atggtaatca tagaagctga tacaaagatt gaacaacatg taccaattgt tccaacaact
    12721 tcaccctcat gagttcctgc ttctacacct gcaattttct gtgccattga tgctgcaggc
    12781 atacgtatat ctccaacaga acctgcaagc caacctatat aagaacctgg agctcctagt
    12841 gtaggataat aagctatagg ttgaacaatc caactaaccc cataagtcgc agcgactaac
    12901 ccccaaattt gaaatataac agaaagcatt ggcatctgac caaagcgaat acctacatag
    12961 atagcgggaa taaagtttgc aataattgca agacctatag aaattctccc tatttttatc
    13021 atctttttat catacataat tatcacctct tatccgtttt aatcacgata atataatctg
    13081 tgtaccaaac ataccaatta acatggcaat acctaaggat aattcttgta atcgttgatt
    13141 ctttttaaat aaagttgtca atactagcat aactatagct gaaataattg ctggtgtaag
    13201 ttttggtatt tcaaatgttt ttatttgatt tgaaagaagg taagcaaata atccaactgt
    13261 agctcctgca agtagttttt taacccaagt aggattatac ttcatgttca tcttttcaac
    13321 aattccacgc attctatgac ccaaaataaa tacaacaaat agccaaccta agttatttaa
    13381 agccatcccc catagtgcat acgaaaaagc ttctgctcca aatgcagcac tacctacttc
    13441 taccccagat aaattggatg taagagatat tactccaagc tctgttctag ctgctccgac
    13501 atcacagagc ctcatccaag tcgtaggagc gccaatcaca gctaccagtg ataataatgt
    13561 aataacagga gatagagaag gtccaatggc tgtaacacta gctgagcgaa ttgctgcttt
    13621 aattcctttc ttttcaatac caagatttga agcctctttt agtgcctctt tcataaatac
    13681 gattgcttga acaactgaaa taattactaa aaaactagaa acaatccaaa gacctgggct
    13741 attaatcatt tcttgaaact tcatacttta ttcctcctta aaataaattt ttcagcattt
    13801 tattgtgagc tttctttctg taatcttgtt atgtttttat attgttttaa tttattttat
    13861 ctaagatata gtatctttaa taggagatta tctgtcttaa agtctcacta aaaacattaa
    13921 tacttaaata ttttattcat aattagtaaa tatcagaaaa ttcacatatt atttttttaa
//
LOCUS       DAEMIK010000119        13879 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEMIK010000119 DAEMIK010000000
VERSION     DAEMIK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.120"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            229..684
                     /locus_tag="KPK87_002836"
     CDS             229..684
                     /locus_tag="KPK87_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG2538968.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            733..1317
                     /locus_tag="KPK87_002837"
     CDS             733..1317
                     /locus_tag="KPK87_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG2538969.1"
                     /translation="MKILVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1322..1645)
                     /locus_tag="KPK87_002838"
     CDS             complement(1322..1645)
                     /locus_tag="KPK87_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538970.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1797..2315
                     /locus_tag="KPK87_002839"
     CDS             1797..2315
                     /locus_tag="KPK87_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2538971.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYEAGTPESPDRHNKTR
                     LPLNEIVSYETL"
     gene            2809..3552
                     /locus_tag="KPK87_002840"
     CDS             2809..3552
                     /locus_tag="KPK87_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2538972.1"
                     /translation="MNKIIEVINLQKSYDTFKAVKRINFYVEEGNLFALLGANGAGKS
                     TTIDILCTLLDYDSGEVIIDGHKLSVEDNKIKEKIGVVFQESILDPLLTVRENLTFRA
                     KFYIKNKGKVEQIVHESALSVGAMEFIDRPYGKLSGGEKRRVDIARALLNTPKILFLD
                     EPTTGLDPEARKCVWDTILKLQKQYGFSIVLTTHYMEEASKADYIVMLDYGTITAKGS
                     PHELKHKYRANNLDDVFLNIVRNRGEKNV"
     gene            3545..4402
                     /locus_tag="KPK87_002841"
     CDS             3545..4402
                     /locus_tag="KPK87_002841"
                     /inference="COORDINATES: protein motif:HMM:NF013245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2538973.1"
                     /translation="MFNLAQRNIMIFLRNKRGVFSSFLGAIIVIGIYVIFLGENLKEG
                     YPNIKDIDILMNSWAMAGIISVVSVTTTMGSFGITVLDKELKRTKDFYCSPIKTRELV
                     GGYILSSYLIGIIMSIFTFVLAEIYIVLSGGQILSMFSIIKVIGVILLTVLSSSAMLF
                     FIINLLNSIDTFATASTIIGTLIGFLTGIYIPIGSLPQAVQTIIKVFPISHGTVLLRQ
                     IITDSPSKVVFVDSTNALTAFNEHMGVTFGSIGSLSETSIHIMFLVISSIIFYFVTII
                     MVSKKKTKN"
     gene            4538..4897
                     /locus_tag="KPK87_002842"
     CDS             4538..4897
                     /locus_tag="KPK87_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG2538974.1"
                     /translation="MIKLIFNIKMQRESLIAYKNEQLYRINPLDIYYFESIDNHTYLY
                     CKNDVYEFKKKLYQIEEIFSNGDFLRISKSVILNLTKIDSIVPAFNGRFEAILDNGEK
                     TIISRQYVPMLKKKLRI"
     gene            4913..5356
                     /locus_tag="KPK87_002843"
     CDS             4913..5356
                     /locus_tag="KPK87_002843"
                     /inference="COORDINATES: protein motif:HMM:NF022896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 family protein"
                     /protein_id="HBG2538975.1"
                     /translation="MNLINTLKNVVRYFCMITTGVLISVFTSCLVFSPEETFNISIFQ
                     QILLVALVTSLSTLIFYSKRELGRKEMIIRQVIHIIFIFVFVLWFSFKSKWVDGGHIF
                     QALFIALSIFIIYICITLATCKRDKKESILLNKGLKEYRKNKINK"
     gene            5514..5972
                     /locus_tag="KPK87_002844"
     CDS             5514..5972
                     /locus_tag="KPK87_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2538976.1"
                     /translation="MEIIDYIEENLTENLNLDKIADIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQKFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5969..6553
                     /gene="clpP"
                     /locus_tag="KPK87_002845"
     CDS             5969..6553
                     /gene="clpP"
                     /locus_tag="KPK87_002845"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG2538977.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6865..7941
                     /locus_tag="KPK87_002846"
     CDS             6865..7941
                     /locus_tag="KPK87_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2538978.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDFTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            8008..10011
                     /gene="bclA3"
                     /locus_tag="KPK87_002847"
     CDS             8008..10011
                     /gene="bclA3"
                     /locus_tag="KPK87_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG2538979.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGLTGADGLVGPTGATGADGLVGPTGPTGATGVGITGATGATGPTGATGATGADGVA
                     GPTGATGATGNTGADGATGPTGATGPTGADGLVGTTGATGATGLAGATGATGPIGATG
                     PTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGPTGASAIIPFASGIPL
                     SLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSIS
                     AYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGVLSVGSISSG
                     IVTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            10180..10941
                     /locus_tag="KPK87_002848"
     CDS             10180..10941
                     /locus_tag="KPK87_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG2538980.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            11250..11528
                     /locus_tag="KPK87_002849"
     CDS             11250..11528
                     /locus_tag="KPK87_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538981.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            11821..12396
                     /locus_tag="KPK87_002850"
     CDS             11821..12396
                     /locus_tag="KPK87_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG2538982.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12835..13545
                     /locus_tag="KPK87_002851"
     CDS             12835..13545
                     /locus_tag="KPK87_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2538983.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            13539..>13879
                     /locus_tag="KPK87_002852"
     CDS             13539..>13879
                     /locus_tag="KPK87_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538984.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVF"
BASE COUNT         5338 a         1650 c         2526 g         4365 t
ORIGIN      
        1 attgttgaat tttcaatgat atattaaggt tctaatatat tatacaaaat aaaaacatta
       61 attcaactta agtaatttta agtatcatat gtgttttgat taaatagacc taaaatttta
      121 agggtaaata acatattcac attaaatttt attgaaatga ttaaaattat ttacttaatg
      181 taaattatta gctaaaattg tactaaataa ttaaggaggt gagtagcttt ggatgagata
      241 gatttaaaaa taattaattc tctaaaaaag aatagtaggg cttcaacctc tgaaataagt
      301 agacaaatta atttatcagt accagcagtc tcagagcgta taaggaaaat tgagcagtca
      361 aatttaattc aaaagtacac tattcaaatt aatcgagaaa aaacaaaata taagcttttg
      421 gcttttatat ttatcaatac tgatttatct aaaagtatga aagaatttaa gaaaactata
      481 ttggaatatg atactgtatt agaatgtcat catatttcag gagagtatga ttacttaata
      541 aaagtattag cagaagatac aagtaatctt gaagagttta tatcagtgac tttaaaggaa
      601 attggtggga ttcagaaagc taatacaacc attgttttat ctacaattaa agaagaattt
      661 aatacaataa aagatatttt ttaaataatt gactagtaaa gtgttaatta tattttagga
      721 ggaaaataga atatgaaaat tttagttttt ttagcaaaag gttttgaaac tatggaattt
      781 agtgtatttg tagatgttat gggatgggct cgtaacgatt atgggcatga tattgatgta
      841 gtgacttgtg gatttaaaaa acaagttatg agcacattta atatacaagt attagttgat
      901 aagacgatag aagaagtatg tgttgatgac tatgatgctc tagctattcc tggaggattt
      961 gaagaatttg gattttatga tgaagcatat gattcatctt ttttaaatct aataagggag
     1021 tttaattcaa aagaaaaaat aatcgcttct atttgtgtgg cagcattgcc agttggaaaa
     1081 agtggtgttt taaaaaatcg taaagctact acatatcatt tgaaaaatgg taaaagacaa
     1141 agacagttaa gtgagtttga tgtaaatgta gttaatgaac caattgttgt agataaaaat
     1201 attataacct catattgtcc agaaacagct ccacatgtag cttttaagct tttagaaatg
     1261 ttgacatcta aagaacaaat ggatgaagtt aaattagcaa tgggatttaa actgtaatgg
     1321 tttatagatt ttctaagtaa tcagaacccc agtcacacat ggaatctaaa attggaataa
     1381 tactctttcc aaaatcagat agcgagtatt ctactttcgg aggaacaaca ggataaacca
     1441 ctcttataat taaaccatct gattctaatt cacgtaattg ttgcgttaac atcttaggcg
     1501 ttgctttagg aattaatttt tttaattcat tgaacctcaa tatagtgtct ttaagatgcc
     1561 aaagtataag tgtcttatat ttaccaccaa tcaatgcaag tgttgcttct attgggcaac
     1621 tgtaattatc taaacatttt ttcatttttg ggctccttta tttattttaa agtatctttt
     1681 taggtactat attacaaaaa agtgcattat tgtaatatag taccatagtg ataaaattat
     1741 agcataggtt aaataaatca tcaacttagc ctaataaaac aataggaggt aaataaatga
     1801 atttcgttga acttgcaaaa aaaagatatt cttgccgtaa ttatcaagat agaaaggttg
     1861 aaaaagaaaa acttgagaag gttttagatg ttgcaagaat agctccaact ggtggtaatc
     1921 gtcaacctca aagattgatt gttattcagg aaaaagaagg tataaataaa ctttctaagg
     1981 ctgccaatat atatgatgcg ccattagcaa ttcttgtatg tggagataaa gacaaagttt
     2041 ggacaagacc ttttgatggg aaacaactta cagatattga cacaagtata gttacagacc
     2101 atatgatgtt acaagcaact gaacttggat tggcaagtgt ttgggtgtgc tattttaatc
     2161 cagatataat tagagaagag tttagtttgc cagataatct agaaccaata aatatattat
     2221 tgatgggata tgaggctgga acaccagaaa gtccagatag acataataaa actcgtctgc
     2281 ctttaaatga aattgtttct tatgaaactc tttagatata aaagcaagtt atctatatga
     2341 ggtatataat gagttttttc aaaataaagt cttagaaaaa gttaatactt atataaaatt
     2401 aattttagtg aagtatatat ctaattgaat aatgatgtat acttcactat tttaattgct
     2461 ctaaatatcc aacaatattt tgaatatcta ataaacttaa actttcattc tattagctaa
     2521 aatacataac ttaaattaat ttttaagtct ggagaatcac ttaaaatata acttttttac
     2581 tttacttggt gtttttgaca aaatctagat tctcttttat tttatgatat aatcttaaca
     2641 agttaaaagt ggtaaagttt ttacaacaat ttatttttaa gtatgtattt tactttagtt
     2701 tttttacaac tcacatatcc atttacacaa catgtttcca attgtattgt gtagacaaat
     2761 tacttattgt attcttaaat catcaattaa ggaaggtaga tgaaatggat gaataagata
     2821 atagaagtaa ttaatttaca aaaaagctat gatactttta aagctgtgaa aaggattaat
     2881 ttttatgtag aggaaggtaa tttatttgca ttactgggag ctaacggtgc tggaaaatct
     2941 acaacaattg atattctttg tacattgttg gattatgata gtggagaagt gattatagat
     3001 gggcataagt taagtgttga ggataataaa attaaagaaa aaattggagt agtatttcaa
     3061 gaaagtatat tagacccact tttaacagta agagagaatc ttacatttcg tgcgaagttt
     3121 tatattaaga ataaaggtaa agtagaacaa attgtacatg aatctgcttt atctgtaggg
     3181 gctatggaat ttatagacag accatatggt aaattgtctg gaggtgaaaa gcgtagggtt
     3241 gatatagcaa gagctctttt aaacactcct aaaatactgt ttttagatga gcctacaaca
     3301 ggacttgacc cagaagctag aaaatgtgta tgggatacta ttttaaaatt acaaaagcaa
     3361 tatggttttt ctatagttct tactacccat tatatggagg aggcttcaaa agctgattac
     3421 attgtaatgc ttgactatgg cactataaca gcaaaaggtt cacctcatga actaaagcat
     3481 aaatacagag caaataattt agatgatgta tttttgaata tagttagaaa tagaggtgaa
     3541 aaaaatgttt aatctagcac aaagaaatat aatgattttt ttaagaaata aaagaggagt
     3601 attttcatct tttctaggtg caataattgt aattggcata tatgttattt ttttaggtga
     3661 aaatttaaag gaaggatatc caaatattaa agacatagat atattaatga acagttgggc
     3721 aatggcagga attatttctg ttgtttctgt aaccacaaca atgggctcat ttgggattac
     3781 agtattggat aaggaattaa aaagaactaa agatttttat tgttcaccaa ttaagacaag
     3841 agaactggta ggaggatata ttttaagttc atatttaatt ggtataatta tgagtatatt
     3901 tacatttgtg cttgcagaga tatatattgt attaagtggg ggacaaattt tatcaatgtt
     3961 ttctattata aaagttattg gtgtaatatt attaacagtt ttatcatcta gtgcaatgtt
     4021 attttttatt ataaatttgt tgaatagtat agatactttt gcaactgctt ctacgattat
     4081 aggtactctt attggattct tgacaggaat ttatatacca attggaagct tgcctcaagc
     4141 agtacaaacc attataaagg tatttccaat ttcacatggt actgtattac ttagacaaat
     4201 tattacagat tctccatcaa aagtagtctt tgtagattca acaaatgcat taactgcctt
     4261 taatgaacat atgggagtaa catttggtag tattggtagt ttgtcagaaa cttcaattca
     4321 tattatgttt ttagttattt catcaataat attttacttt gttactataa ttatggtatc
     4381 gaaaaagaaa actaaaaatt aatagtgcaa aaatataata gagtcttttt atagaaatca
     4441 aaagaaggga tgtgttagag tgaaaataat aattgaaact ccaccagatg gagcagagga
     4501 tgaaattatc ataagatgca gaaatcttga tgatgaaatg attaagttga tttttaacat
     4561 taagatgcaa agagagtctt tgatagcata taaaaatgaa caactctatc gtatcaatcc
     4621 tttagatatt tattactttg aatctataga taatcatact tatctttatt gtaaaaatga
     4681 tgtttatgaa tttaagaaga agctttacca gattgaagaa attttttcaa atggtgattt
     4741 tcttagaata tcaaagtcgg taatattaaa tcttacaaag atagattcga ttgtaccagc
     4801 atttaatggt agatttgaag caatattaga taatggtgaa aagacaatta tttcacgcca
     4861 atatgtacca atgttgaaga aaaaactaag gatatagagg agttgattaa atatgaattt
     4921 aataaataca ctaaaaaatg tagttcgtta tttttgtatg ataactacag gagttttaat
     4981 atctgttttt acttcttgcc tagtatttag ccctgaggag acgtttaata taagtatttt
     5041 ccaacagata ttattagttg cattggttac atctctatcc acattaattt tttattcaaa
     5101 acgagaactt ggtagaaaag agatgataat tcgtcaggta attcatataa tttttatatt
     5161 tgtttttgtt ctgtggtttt catttaaaag taaatgggtt gatggtggtc atatttttca
     5221 agcattattt atagcactat ctatatttat tatctatatt tgtataacac ttgctacttg
     5281 taaaagagat aaaaaagaat ctattttatt aaataaaggt ctaaaagaat ataggaaaaa
     5341 taaaatcaat aaataataat taaagcaatt caagaaattg taaatttatt attaattaag
     5401 ttttatatca gtcaataatt taagatagca atttatatta agaagaaaat tcttgcatag
     5461 gttataatac ttaaaaggag aggatagagt tgaaaacaaa tacagatgtt attatggaga
     5521 taattgatta tatagaagaa aatcttacag aaaatctaaa tttagataaa atagctgata
     5581 ttgctaatta ttcaaaatac catttacaca gaatgtttac aaatgttgta ggatgtacta
     5641 tccatcaata catacagagg aggagactta ctgaagcagc tagacaactt atatttacag
     5701 acaaatatat tatagacata gctattacag taggatattc tacacatcaa tcttttacac
     5761 tagcatttag aaatttatat aaagaatctc cacaagtata tagaaataaa gatgaatttt
     5821 atcctattca acagaaattt gttttaaatc taaatcatca aattcaaagc gaaaaatgta
     5881 tttcctatgc aaatatgaat tataaacaaa gaagagatga aatcaattct tatcttaact
     5941 ttaatattaa ttgtgaggtg aaagtagcat gagtttagtg ccacatgtaa ttgaacaaac
     6001 aggtcaaggg gaaaggtctt atgatatata ttcaaaatta ttaaaagata gaattatatt
     6061 tataggagaa gaaattaatg atgctattgc aagtttagta gtagctcaat tactgttttt
     6121 agaatcagaa gacccagatt cagatataat tatatatata aatagccctg gtggctctgc
     6181 tacatcagga tttgctatat atgatactat gaattatgtc agatgtgatg tatcgactat
     6241 ttgtattggt atggctgcaa gcatgagtgc ttttttactt gcaggaggaa cacatggaaa
     6301 aagatgtgct ttacctaatt cagagattat gattcatcag ccaatgggag gagctaaagg
     6361 tcaagcaaca gatgtaaaaa tagctgtaga taatatattg aaaataaaag aacgattaga
     6421 taaaattctt tctgaaaata caggtaaatc aatagaagat attaggagag atacagatag
     6481 agataatttt atgactgcac tagaggctaa agagtacggg ctgattgatt atataatgaa
     6541 taggaatgaa taaagtatca aagtatcata ttaaaatgtc tgttttacaa atatatatat
     6601 aatattaaat aagagtgtct caaaatgaac tttttagttt atgggacact cttttttgta
     6661 tgaaattaaa tatggtttta tcatttcaac aattattacc aaatttcaag tttaataaat
     6721 tctactataa aaaatacata aaagataagg ttctttagta actttctgtc gtcaaaagtt
     6781 tttataaatt taataacaaa acaagagaaa ataccatata ttacttatgt ctgcaatata
     6841 tagaagacaa aggagttgta cattatgatt acaataagtt tgtgcatgat tgttaagaat
     6901 gaggaagaag ttataggcag atgcttagaa tgtgtaaagg atatagtaga cgaaattata
     6961 attgtagata ccggttctac agatttcaca aaagaaatag tctcaaaata tacagacatg
     7021 gtgtacgatt tcgaatggat agatgatttc tctgctgcaa gaaatttttc attttctaaa
     7081 gcttctaaag actatactat gtggcttgat gcagatgaca taattttaga agctgataga
     7141 gaaaaattat taaagattaa agaaagcttg gatacttcca ttgatattgt tatggcaaaa
     7201 tacaatgtat cctttgatga aaatggtaac ccaactctct cgtattatag agaacgattg
     7261 tttaaaagaa gttctaatca taaatgggtt gaccctattc atgaagtagt acctcttttt
     7321 ggagaggtat tttattctga cattgcaata tctcataaaa agcttcatag acaggaccct
     7381 ctaagaaatc ttagaatttt tgaaaaaatg atttcagaag gaaaaacatt ggagccaaga
     7441 caccaattct attattctag agagttgtac tataatgcta ggtataaaga agcaatagaa
     7501 ggttttacta agtttttaga ctcatcaaga ggatggattg aggattgtat cagtgcttgc
     7561 agagatttag ccacatgtta ttacttaatt aatgatgaaa aaagtgcctt atattcattg
     7621 tttagaagtt tcgaatttga tgagccaaga gcagaaattt gttgtgacat aggaaagcat
     7681 atgtttgata gacaaaaata taaagaagcc attttttggt ataaggtagc attaacaaga
     7741 gataagaatg atactaatgg aggttttaag tcaaatgatt gttatggata tataccatct
     7801 atacaattaa gtgtctgtta tgacaggtta ggagaatcag ataaagctat ttattatcat
     7861 gaaaaaacaa aagaaatcaa acctaacgat agtgctgttt tgcacaatga aaattatttt
     7921 agtaaattta aaaatagttg ataggtcaca atcatactaa atttgacata ttatatatag
     7981 agcttaaaag ctcaaatata tcaggaggtg cttttaataa tgagtagaaa taaatatttt
     8041 ggaccatttg atgataatga ttacaacaat ggctatgata aatatgatga ttgtaacaat
     8101 ggtcgtgatg attataatag ctgtgattgc catcattgct gtccaccatc atgtgtaggt
     8161 ccaacaggcc caatgggtcc aagaggtaga accggcccaa caggtccaac gggtccaaca
     8221 ggtccaggag taggggcaac aggcccaaca ggaccaaccg gtccgactgg tccaacagga
     8281 aatacaggga atacaggagc aacaggatta agaggtccaa caggagcaac aggggcaaca
     8341 ggcccaacag gagcgacagg agctataggg tttggagtaa caggtccaac aggcccaaca
     8401 ggagcgacag gagcaacagg agcagatgga gtaacaggtc caacaggtcc aacgggagca
     8461 acaggagcag atggaataac aggtccaaca ggagcaacag gggcaacagg atttggagta
     8521 acaggtccaa caggtccaac aggagcaaca ggagtaggag taacaggagc aacaggatta
     8581 ataggtccaa caggagcgac aggaacacct ggagcaacag gtccaacagg ggcaatagga
     8641 gcaacaggaa taggaataac aggtccaaca ggagcaacag gagcaacagg ggcagatgga
     8701 gcaacaggag taacaggccc aacaggccca acaggggcaa caggagcaga tggagtaaca
     8761 ggtccaacag gagcaacagg agcaacagga tttggagtga caggcccaac aggtccaacc
     8821 ggagcaacag gaataggaat aacaggagca acagggttaa taggtccaac cggagcaacg
     8881 ggagcaaccg gagcaacagg cccaacagga gtaacaggag caacaggagc agcaggatta
     8941 ataggaccaa ccggggcaac tggagcaaca ggagcagatg gagcaaccgg agcgacaggg
     9001 gcaacaggag cgacaggtct aacaggagca gatggattag taggtccaac aggagcaaca
     9061 ggagcagatg gattagtagg cccaacagga ccaaccgggg caacaggagt aggaataaca
     9121 ggagcaacag gagcgacagg tccaacagga gcgacgggag caacaggagc agatggagta
     9181 gcaggtccaa caggagcaac aggggcaaca ggaaatacag gagcagatgg agcaacaggt
     9241 ccaacagggg caacaggtcc aacaggagca gacggattag taggtacaac aggagcaaca
     9301 ggagcaacag gattagcagg agcaacagga gcaacaggcc caataggagc aacaggtcca
     9361 acaggagcag atggagcaac aggggcaaca ggagcaacag gtccaacagg ggcagatggg
     9421 ttagtaggtc caaccggagc aacaggagca acaggagcga ccggagcaac aggtccaaca
     9481 ggcccaacag gtgctagtgc aataatacct tttgcatcag gtataccact atcacttaca
     9541 actatagctg gaggattagt aggtacacca ggttttgttg gctttggtag ttcggctcca
     9601 ggattaagta tagttggtgg agtaatagac cttacaaacg cagcaggaac cttgactaac
     9661 tttgcatttt caatgccaag agatggaaca ataacatcta tttcagcata tttcagtaca
     9721 acagcagcac tttcacttgt tggttcaaca attacaatta cagcaacact ttaccaatct
     9781 actgcaccaa acaactcatt tacagctgta ccaggagcga cagttacact agctccacca
     9841 cttacaggtg tattatcagt tggttcaatt tctagtggaa ttgtaacagg attaaatata
     9901 gcagcaacag cgcaaactcg attcttacta gtatttactg caacagcttc aggtctttca
     9961 ttagttaata ctgtagcagg atatgcaagt gcaggaattg caataaatta gattagataa
    10021 gaaaaggctt ctaatatcgt attgatatta ggagcttttt taagaatgat tgatgaacat
    10081 agttttaata ttaattagtg cgcatattat aaaatactat tatataatat aaaataataa
    10141 cattaaattg gaaatataga attatctagg ggtgatacta tgaaaaataa gattgaaata
    10201 tcaatgaaca actttgtaaa aaattatcat aaatcaaata atataactac aaagtggcaa
    10261 gaacttttgt ttgcttatgc atctgctgaa gatgatatgt ttcagaagct aaaaagtgtt
    10321 gtaggaacat ctcatgctat gccaaaagat tttctggaaa atggaaaaac tataattaca
    10381 tattttatac cttttgatga aagtgtaaat aatagtaata ttggagggtt tgaatgttct
    10441 gaaatatggg caagagcgta catagaaact aatatgttga tttcaaattt aaatgatttc
    10501 ttatgtgaag aactagagaa gataggggct aattcatatg ctattcctgg gtcgtataat
    10561 tttgacagtg aaaaattggt aagtgactgg tcacaaagac atgtagcatt catagcaggt
    10621 cttggaactt ttggtttaaa taatatgctt attacagaaa aagggtgttg tggaagaata
    10681 ggaagcataa tcacaagttt agaattagag ccaactaaaa gaccagaaaa tgaatactgc
    10741 ttgtataaac ataataatag ttgcaaaaaa tgtgtcaaca aatgcgtgaa agaagcttta
    10801 aaagaagatg gatttaatag gaataagtgt tatgaaatgt gtatgataaa tgaaaaaaaa
    10861 tacacttata taggtggatg tgatatttgt ggaaagtgta tagttggagt tccttgttca
    10921 gtaataaatc catcaagata atggacttaa gttgactaat aattttaaag atagtttata
    10981 atgaaaaaat tttagttatc aaaataaata taatataaaa actaaataaa tgtgtttaaa
    11041 aaatggataa ataatttgtg ttgattgaca accagttata ctgttgatag acttatatgt
    11101 atcgctgtga tatataagga gggttgagat tttatgtttg atttaatctt tatttttgca
    11161 tagcaagggc tattgcaact atgaaattta atatataaat agccctttgc tattcctaat
    11221 gaaaaattac tatttaatag gagagcaaaa tgagctataa aaaagcaata catatattac
    11281 cagaagaatt acttgaatta atacaggaat atgtagatgg tgaatgtatc tatattccta
    11341 gaaaatctaa taataaaaaa gaatggggaa gtaatacacc tactcgtgaa gagttgtcaa
    11401 ttagaaatat gcagatttac gaagactatc aagttggata tgatttgcaa tatttatcgg
    11461 aaaagtatta tttatctttg aaaagtatcc aaagaattgt attacaagaa aaaagaaaag
    11521 atatctaagg atgagccagt atagttttat aattatactg gctcattttc ttttgataaa
    11581 agtgtttgag gtatagtttc ttttaagtgg atgatataat ttaagtaatt tcaataactt
    11641 atacagtagc taacaaagct ggttgtaaag tatctaataa gaagtagtag ctttttatat
    11701 gtttgaaatt aaaatttatt aaaattagga ggtcttgttt tgaaaaaaat acttgattat
    11761 attccacctt ttcataactt tctattaatt tagtaatctt atagaaagga aattgcattt
    11821 atgaaaaaaa ttacttggga ggtggaatat atctcccagt tacctgtaat taggtattgt
    11881 aaaaaatgtg gaaaaaagac tgaatatatt tgttcaggtt tatttagggt taatgctcaa
    11941 cataaatatt tggatatatg gttaatatac aagtgttcaa attgtgattc aacatggaac
    12001 tcaactatat attctcgtgt taatccaaaa agcttaaatc cagaaatact agaacaattc
    12061 catacaaacg attataatct tgttaagcaa tatgctatga atatggaact tttacataga
    12121 aatggagcag aagttggtat tccagagtat aaaatagtag gacaagaaat taatattaat
    12181 gaccttacac aagttcatat tattagtaaa tatccatgtc agttaaaggt atcaacatta
    12241 ttacgtgaga aactaggttt atctaggaag gtatttgacg aaatgttgga ttgtgggatt
    12301 attagaagta actcaggagt tgatttaaag aaaggtaaat taaattatga aattatgtta
    12361 gatataggca aggagaagca aaagtcttca aattgattag aataactaac ttaatatatt
    12421 ttcaggaaca tcatttaaaa aaatagtgac tttaaaaaat ttaaagccac tattttttaa
    12481 tatgttttat atgagtattt aaaataaagt gtgtattctt caaatttata gcgataatta
    12541 aaacattact ataaaaagga aggtgcatac tttttattta acaaaaacta gatattaaaa
    12601 acttttcgag gaatatttta ttaaaatccc taaatatatt taatatatat taaaaaattg
    12661 tattataatg aaaatcaaaa tagattgcaa atccaaaaag taatacttaa tgtccaaaaa
    12721 gctaaaatta aaatctaaat agctatttac tacagaagta tgttaaaata tgtaaaatga
    12781 atagaaattt atatattttt attaaaaata tataagaaag gatgtttaag aattgtgatt
    12841 aatataggaa tatgtgatga tgaactacat tatcgaataa aaataaaaga tatattaagt
    12901 gaaatattaa gttcatatcc tataaattat aacatatatg aattttcagc aggagaagag
    12961 ttactaaata actatcctaa agatttagac atattaatta tggacataca aatgaaaaca
    13021 ataaatggta tggatactgc taggaagata agagagtttg atcataagtt agaaataata
    13081 tttgttactt catttgtaga gtttatgcaa gaaggttatg aggtaaaagc atacagatat
    13141 atactaaagc caataaataa agaaaaaatt tctaaaagtg tattaccttg tataaatgag
    13201 atgatgaaaa agagaaataa ttacttaact ataaatgtaa aaaattatgt agaccgaatt
    13261 aaaatagatt ctattactta tatagaaaca gatagaccga atatattgat ttatacacat
    13321 gatgatatgt atattactaa aatgagtata tctaaaatag aaaagatatt aaatgaatat
    13381 ggatttttta gatgtcataa tagctatata gttaacttaa aattagtaga atcaatgagt
    13441 ggaagtactg ttatagtaga tggaaaaagt attccaataa gtaaatatag ggttaaaggc
    13501 ttgaagcttg cgataactaa tatattaggg gacatagtat gctaacaaat ttaagtgtag
    13561 gattttggaa tattattaat ttagtttcag gtactataga ttggattata ttttttttag
    13621 taatccactt agttggaaag aggaaaatat ctaatataag atatatattt acaacaataa
    13681 ttataattat atttatggca tacataaatg tagttaaagt gtttccaaat attaagattc
    13741 ttctatgtat ggtaatagga atatcatttt atataacatc atatgaagat aaaatctata
    13801 aaagtataat agttagttta ttgttttggc taggattaat ggttgctgaa ggttctgcag
    13861 taggtctagt agtatttat
//
LOCUS       DAEMIK010000120        13837 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEMIK010000120 DAEMIK010000000
VERSION     DAEMIK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.121"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            295..1134
                     /locus_tag="KPK87_002853"
     CDS             295..1134
                     /locus_tag="KPK87_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG2538985.1"
                     /translation="MLDINSFLNGFFNIILRMSIYGLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1131..2312
                     /locus_tag="KPK87_002854"
     CDS             1131..2312
                     /locus_tag="KPK87_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538986.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2616..3518
                     /gene="ptb"
                     /locus_tag="KPK87_002855"
     CDS             2616..3518
                     /gene="ptb"
                     /locus_tag="KPK87_002855"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG2538987.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3540..4619
                     /gene="buk"
                     /locus_tag="KPK87_002856"
     CDS             3540..4619
                     /gene="buk"
                     /locus_tag="KPK87_002856"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2538988.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIKDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4609..5286
                     /locus_tag="KPK87_002857"
     CDS             4609..5286
                     /locus_tag="KPK87_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2538989.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5329..5544
                     /locus_tag="KPK87_002858"
     CDS             5329..5544
                     /locus_tag="KPK87_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2538990.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5573..6652
                     /locus_tag="KPK87_002859"
     CDS             5573..6652
                     /locus_tag="KPK87_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2538991.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6652..7404
                     /locus_tag="KPK87_002860"
     CDS             6652..7404
                     /locus_tag="KPK87_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2538992.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7405..7962
                     /locus_tag="KPK87_002861"
     CDS             7405..7962
                     /locus_tag="KPK87_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2538993.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8132..9478
                     /locus_tag="KPK87_002862"
     CDS             8132..9478
                     /locus_tag="KPK87_002862"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG2538994.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9745..11577
                     /gene="glmS"
                     /locus_tag="KPK87_002863"
     CDS             9745..11577
                     /gene="glmS"
                     /locus_tag="KPK87_002863"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG2538995.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     QNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12029..13324
                     /locus_tag="KPK87_002864"
     CDS             12029..13324
                     /locus_tag="KPK87_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG2538996.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYLDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
BASE COUNT         5499 a         1528 c         2721 g         4089 t
ORIGIN      
        1 tattaaacaa agatatatta agatatttta gaacacaaaa ctgcattaag gcgcgagtac
       61 tattttgtat tcgcgccttg atggtttgag ttatttgagt aaaaactaac tttaataaga
      121 aatctgtatt tattaagttg atatttttaa tgtgtttaga gattatttaa caatctccaa
      181 atataaaact agaattattt acataaaagt taaatagttt gtatagagtt agatttatat
      241 agtataatac ttagtgtaaa taattaagaa gttatcaaac aggaggataa aactgtgtta
      301 gatataaatt cttttttaaa tggatttttt aatataatat taagaatgag tatatatggt
      361 ttaattgaca tatctatagt agcgtatatt ttttacaaaa tatttatgtt tataaaagat
      421 acaagagcag aacaagtgtt taaaggtata atatttcttt tattagcaac tcaacttagt
      481 aacaccttta aattgcatac cgtttattgg atatctctta aagctttaga ttatggagtt
      541 atagctgctc ttataatttt tcaaccagaa tttagagcag gacttgaaca tataggaaga
      601 gcaaagttta atttatttgg aaagaatgta aatacttctg aagaaactct caatagaaat
      661 atagaagaaa ttgtagaagc tttatattca ctttctagac aaaaaatagg agctcttatc
      721 ataatggaaa gagaaactag aataagtgat ataataaata caggaactat tattgatgct
      781 gaaatttcta ggcaactatt aataaatata tttataccaa atacaccatt acacgatgga
      841 gctgttgtaa ttagagattc taaggtaaaa gcagctgcat gttttttgcc attgacagaa
      901 agtaaggatt taagtaaaga tttgggtact agacatagag caggaatagg ggtaagtgag
      961 gtgtctgact gtataacttt gattgtatca gaggaaacag gtggcgtatc aattgctaaa
     1021 gcaggtaagt tatatagaga tatttctaga gaaagaatga tgaatatatt acgtagtaat
     1081 ttaaaaacaa atacagaaac aagaagtttt ttcaaaggtg gtatatttaa atgataaata
     1141 gactaaaaaa taataccaaa ataaaaataa tatcattttt aagtgcaatt gcattatgga
     1201 tgtatgttat ggctgtagtt gaccctgaag aaactaaagg gtttgaagat atacctgtat
     1261 ctataagtaa tatgagtgat ttaaaggata aaaatcttgt tatatatcca gacgaaaaat
     1321 taacagcaga tgtatatata aaaggtagtt tatcaaaggt acaaagtgtt aaaaaagaag
     1381 atatacatgt atatggaacc attgaagacc caatacaagg acagaaggaa gtatatttaa
     1441 aagcaaatat tcctcaaggt gttactctag aatttaagca tgatgtttta gtagtaaatc
     1501 ttgaaaaaaa cataaaagaa aaaagagata taaaggtaga agtaagtgga aattcaaaaa
     1561 gaaatataaa taaaattgaa cttagtaagg aatttgtcga tgtatcaggt ccaagaactc
     1621 tagttaaaga agtcaaaagt gtaagagcta atctagatgt tggtaatgag acagatgatt
     1681 tttctaaaaa attgcaatta ataccattaa atagtgatgg aactaaggtt gaaggagtaa
     1741 cattaagttc atcctttgtg actgcaaaga tagaactatt gaaggaaaaa attgtcccaa
     1801 taagagtaag tctaaatgat aataaagata atgatgtact gaaaaactat aaattaagta
     1861 aggaacaagt tacaataaaa ggaaagaaag aagatataga caaaataagt tatattaaga
     1921 caaaacctat tgacttagct gatttggcta atggtgaaga aaaagaagtt agcttagaaa
     1981 ttccagaggg tataactctt gaggatagct ctataacaat taaattaaat gatacaaaac
     2041 aattgagtgc agaattttta tataatgcgg gagatattga attaagaaat atcccaacaa
     2101 atatggataa tccaaatgta atttctgatg tagatataaa agttgttgta gagtcaacgg
     2161 aggatttgag taagttaaat aaaaaagata tttcattata tattgattta tcacaaggcc
     2221 ctgaggaaga tagtaaatat aaaataaaat atgaaacaac atatcaattt aaaaaaataa
     2281 atatagaacc taatattgtt gaagtggaat gaacaaatat ttcgaatatt tagttgttac
     2341 aagatgcgac aaaaaaacct ataaaattaa gtttgttaat tttatgtttt tgttgcatct
     2401 tgtttatttt agttatatta taaaataagg acaatgagat atttgttaaa tctaaaaagc
     2461 gctagatata tgaaaaaaga ctgataaata gatttaaata aatattttga attaaatatt
     2521 tcaattatct ctaggaaaac gttgctgaac gagttataat ttaattgaga tataaaaagt
     2581 tctatttcaa aaaattcaag atgaggtgga aatgcatgag aagttttgaa gaagtaatta
     2641 agtttgcaaa agaaagagga cctaaaacta tatcagtagc atgttgccaa gataaagaag
     2701 ttttaatggc agttgaaatg gctagaaaag aaaaaatagc aaatgccatt ttagtaggag
     2761 atatagaaaa gactaaagaa attgcaaaaa gcatagacat ggatatcgaa aattatgaac
     2821 tgatagatat aaaagattta gcagaagcat ctctaaaatc tgttgaatta gtttcacaag
     2881 gaaaagccga catggtaatg aaaggcttag tagacacatc aataatacta aaagcagttt
     2941 taaataaaga agtaggtctt agaactggaa atgtattaag tcacgtagca gtatttgatg
     3001 tagagggata tgatagatta tttttcgtaa ctgacgcagc tatgaactta gctcctgata
     3061 caaatactaa aaagcaaatc atagaaaatg cttgcacagt agcacattca ttagatataa
     3121 gtgaaccaaa agttgctgca atatgcgcaa aagaaaaagt aaatccaaaa atgaaagata
     3181 cagttgaagc taaagaacta gaagaaatgt atgaaagagg agaaatcaaa ggttgtatgg
     3241 ttggtggacc ttttgcaatt gataatgcag tatctttaga agcagctaaa cataaaggta
     3301 taaatcatcc tgtagcagga agagctgata tattattagc cccagatatt gaaggtggta
     3361 acatattata taaagctttg gtattcttct caaaatcaaa aaatgcagga gttatagttg
     3421 gggctaaagc accaataata ttaacttcta gagcagacag tgaagaaact aaactaaact
     3481 caatagcttt aggtgtttta atggcagcaa aggcataatt ttaaggggga aataggcaaa
     3541 tgagcaaaat atttaaaatc ttaacaataa atcctggttc gacatcaact aaaatagctg
     3601 tatttgataa tgaggattta gtatttgaaa aaactttaag acattcttca gaagaaatag
     3661 gaaaatatga gaaggtgtct gaccaatttg aatttcgtaa acaagtaata gaagaagctc
     3721 taaaagaagg tggagtaaaa acatctgaat tagatgctgt agtaggtaga ggaggacttc
     3781 ttaaacctat aaaaggtggt acttattcag taagtgctgc tatgattaaa gatttaaaag
     3841 tgggagtttt aggagaacac gcttcaaacc taggtggaat aatagcaaaa caaataggtg
     3901 aagaagtaaa tgttccttca tacatagtag accctgttgt tgtagatgaa ttagaagatg
     3961 ttgctagaat ttctggtatg cctgaaataa gtagagcaag tgtagtacat gctttaaatc
     4021 aaaaggcaat agcaagaaga tatgctagag aaataaacaa gaaatatgaa gatataaatc
     4081 ttatagttgc acacatgggt ggaggagttt ctgttggagc tcataaaaat ggtaaaatag
     4141 tagatgttgc aaacgcatta gatggagaag gacctttctc tccagaaaga agtggtggac
     4201 taccagtagg tgcattagta aaaatgtgct ttagtggaaa atatactcaa gatgaaatta
     4261 aaaagaaaat aaaaggtaat ggcggattag ttgcatactt aaacactaat gatgctagag
     4321 aagttgaaga aagaattgaa gctggtgatg aaaaagctaa attagtatat gaagctatgg
     4381 catatcaaat ctctaaagaa ataggagcta gtgctgcagt tcttaaggga gatgtaaaag
     4441 caatattatt aactggtgga atcgcatatt caaaaatgtt tacagaaatg attgcagata
     4501 gagttaaatt tatagcagat gtaaaagttt atccaggtga agatgaaatg attgcattag
     4561 ctcaaggtgg acttagagtt ttaactggtg aagaagaggc tcaagtttat gataactaat
     4621 gataaaagaa taagaatcat aactggtcac tatggaagtg gtaaaagtga atttgcgatg
     4681 aactatgttg ttaagttaag agatatggtt tctggcaaag ttgctatagc cgatttagat
     4741 gtggtaaatg tatactttag aactagagaa aagaaagagt taatgaaatc cttaggtata
     4801 caaccaatag atagctcaat taatgctcca acattagatt taccagcagt atcagcagag
     4861 gtaatgtctc ctatggtaga ccattcatac aattctgtta tagatttagg tggagataat
     4921 gtaggtgcta gggttatagg aagatttagt catttgttaa aagaaggcga ttatgatatg
     4981 ctttttgtaa taaatgcaaa tagggagaaa actcaaactt ctgaagaagt tatccaatat
     5041 ataaaagaaa tagaaaaatc ttctaaatta aaggtaacag gtctaattaa taatactcat
     5101 cttattaggt ttactactat tgatgatgtt ttaagaggtc agaaagttgc aaaagaagtt
     5161 tctgaaaaat gtaatatacc gataagatat gttgcatgtt tagaaaatct tgttgaacag
     5221 ttaccaaaag atttagaagg agaaattttt cctataaaat tatatatgag agaagactgg
     5281 atgtagtttg agctcaaata tatagaaagt ttaaggaggc ttaggtaaat ggctaaagga
     5341 aaagtatctt ttaatcaaga acgttgtaaa ggttgtggat tgtgtgttga agcatgtcca
     5401 gtaaaaatca tacaattaga ctcaaatgta ataaacaaaa aagggtataa cccagcaact
     5461 gtttttgaaa tggaaaaatg cataggatgt gcaagttgtg caactatgtg tccagatgta
     5521 gttataacag tagaaagaga ttaatttaat taatatttaa gggggaattt taatggctaa
     5581 gatacttatg aaaggtaacg aagcatttgg aaaagcagct atagaagctg gttgcaaata
     5641 tttcttcggt tacccaataa ctccacaaag tgaattacca gaatatttat caagagagtt
     5701 acctaaaata ggtggagcgt tcgttcaagc tgaatcagaa gtttctgcga taaacatggt
     5761 ttatggtgga gcaggtgcag gagcaagagt tatgacttct tcatcttcac caggagttgc
     5821 tttaaagcaa gaaggtataa catatgcagt aggagcagaa gttccttgtg ttgtacttaa
     5881 tgtaatgaga ggtggtccag gacttggaag tatacaacct tcacaagctg actactttat
     5941 gtctacaaga ggtggaggaa atggagacta cagaactcca gtattcgcac cagctacagt
     6001 tcaagaagct gttgatatga taatggaagc ttttgacgtt gcagattatt acagatcacc
     6061 agttatggta gttgcagatg gtatgatagg tcaaatgatg gagccagttg aatttagagc
     6121 tcctgagaag aaaagagaat tgcctcctaa agattgggca actgttggaa ctaaaggaaa
     6181 aagaaaacct aatgtaataa actctctata cttagagcca gaagtactag aagaccattg
     6241 ttggcattta caagaaaaat ttgatgcaat ggaaaagaat gaagtacaat acgaaatgta
     6301 caaaacagaa gatgctgaat ttgtatttgc agcttatgga actacttcaa gagtagtaaa
     6361 aagcgcaatt gatatattaa gagaagaagg aataaaagca ggtcttataa gacctaaagt
     6421 attatggcca ttcccattcg aagcattcaa ccaaattcct aatgctagaa acatattgac
     6481 tgttgaaatg agtatgggac aaatggtgga agatgttaaa atggcagtag aaggtaaatt
     6541 accagtttac ttccatggaa gaccaggagg aatgactcca acaccagcag aaatagttga
     6601 aaaagctaaa aaaattattg ccggagaatt ggttgcagga ggtgctagat aatggctgtt
     6661 gtatttaaga aaactgaagg attacaagat actcaaactc actactgtcc aggatgtaca
     6721 catggtataa tccatagatt agttggtgaa gtattagaag aactaggagt attaggtgat
     6781 gctgtaggtg ttgttccagt tggatgttca gttttaggat ataaatattt taactgtgac
     6841 actcaagaag ctgctcatgg tagagcgcca gctgctgcta caggaataaa aagagttcat
     6901 cctgaaaata cagtattcac ttatcaaggt gatggagact tagcttcaat tggtactgct
     6961 gaaatagttc atgcagctgc tagaggagaa aaaataacta ctatatttgt aaacaataca
     7021 acttatggta tgactggtgg acaaatggct ccaactacat tagttggaca aagagctact
     7081 actgctcaat caggaagaaa tgctgaaact caaggttatc caataagagt aagtgaaatg
     7141 ttagctacat taacaggagc tgtatttgta gaaagagtag cagttgatac tccagctcat
     7201 gttagacaag ctaaaaaagc tataaagaaa gcattccaag ttcaacaagc aggattagga
     7261 tttggaatag tagaagtatt atctacttgt cctacaaact ggggactagc tccaaatgat
     7321 gctttacaat ggttaagaga caacatgata ccatactatc cattaggaaa tttcaaaaat
     7381 gttgaagttg aggaggtgaa gtaaatgtct acagctagag taatatgtgc aggcttcgga
     7441 ggccaaggtg ttatgtctat gggacaatta cttacttatg caggaatgct agaaggaaaa
     7501 gaagtttctt ggttaccatc ttatggacca gaaatgcgtg gaggtacagc aaactgtgca
     7561 gtaactgtat ctaatgagcc agttggttct ccacttataa cagatgatgc aactgcagct
     7621 atattattaa atataccagc atttgaaaaa ttcaaagatg atgttattcc tggtggaaaa
     7681 ataatagtaa atagttcttt aataaaagaa aaagttgata gaacagatgt agatgtttat
     7741 tatataccag caaatgaatt agcagctgaa ttaggaaatg ataaagttgc taacatgata
     7801 atgttaggcg cttacttaaa agtatctgat actgtagata tagaatctgt actagaagca
     7861 ttcaaaaaag ttttcggacc tagaaaagaa aaattcgttc cattaaacag agaagcttta
     7921 caaaaaggta tggatgctac atgttctaaa gcaactaact aataaagaaa aagatgtcaa
     7981 aaaaaagaca atagaattat ttctattgtc ttttttgctg ataagtatta aaatattttt
     8041 atgactgaaa ttaagcctat aatgatattt tatgaactat aaaaatataa tacaaattac
     8101 aagcttttca attatgaaag gagaatcttt agtgagaaag tattttggaa cagatggagt
     8161 tagaggagta gcaaatacag aactaacatg tgatttagca tataaacttg gtagagctgg
     8221 tggatttgtc ttagctcaag gagaccacag agtaaaagta gtagtaggta aggatactag
     8281 aatatcaggt gatatgttgg aagcatcttt aatagcagga cttatgtctg ttggatgtga
     8341 tgttattact gttggaataa taccaacacc tgcagtggca tatttaacta gaaaatatgg
     8401 tgctgattgt ggtgtggtta tatctgcatc tcacaatcct gttgaatata atggaatcaa
     8461 attctttaat aagaatggat ataaattaga tgatgaaatt gaattgaaaa tagaagaata
     8521 tatagacgat atagacaaaa ttgattgtct tccaattggt gaaaatgtag gaagaaaatt
     8581 acatgaacat tgtgcacaaa gagactatgt agactaccta aaatcaataa taagcactga
     8641 ctttaaggga ttaaaagtag ttttggactg tgctaatggt gcgtcatata aagtggcacc
     8701 aatagtgttt gatgaactag gagcaagtgt aatttcaata aatagttcac cagatggaaa
     8761 taatataaac tataaatgtg gctctacaca tccagaacaa cttcaaaggg ctgttttgga
     8821 acataatgct gacttaggtc ttgcatatga tggagatgct gatagattaa ttgcagtaaa
     8881 tgaaaagggt caaattgttg atggagacca tataatgata ttgagtgcct taaatttaaa
     8941 gaaaaataat aaattggcac aagatacttt ggtagttaca gttatgagta atatagggtt
     9001 gacaatagct gctaaggaaa atggaataaa tttatctact acagcagttg gagacagata
     9061 tgttttagaa gatatggtta aaaatggata taatcttggt ggagagcaat ctggacatat
     9121 gatattttta gattacaaca ctactggaga tggagtgctt agttctctaa tacttgcaaa
     9181 tataatcttg caagaaaaga aacctttatc agaaattgca tcaataatga gccaataccc
     9241 acaagtatta gtaaatgcaa caataaaaaa tgaaaataag aacaaatata tggaatatcc
     9301 agaaataaaa actgagatag aaagaataga atctatttta gacggaaatg gtagagtttt
     9361 gataagacca tcaggaactg agccattagt tagagttatg ttagagggta aagaggaagg
     9421 tcaaataaaa gaattagcaa caaacttagc taatcttata caagaaaaat tatcgtaatt
     9481 taaagggtat tctcagatat aaatactgag gacacctttc taagcgccag aacttagtta
     9541 tagacataat tattgagtaa tataggaact aagttgacga ggtcagagtt aatcgaattg
     9601 tcggcggatg ctctgcggtg tgtttaccat cgaaagactt ctttaaataa taggagcaat
     9661 cctatggaca aagggaagcc aaagtaaact tatatgacct aactcttaaa atatataaat
     9721 aatattttta ggaggacaca aataatgtgt ggaatagttg gatatttagg aagtagaaag
     9781 gcagcagaag ttatagtaga agggctttct aagctagagt acagaggtta tgactcagca
     9841 ggagtagcag ttaatagttc taatgaaaaa gaacttaata ttagaaagtt taaaggtaga
     9901 ctttctgtac ttgcagaaga cttagaaaag aatcctattg atggaaattt aggtataggg
     9961 catacgagat gggcaactca tggagaacca tctgatgtaa actcacatcc tcattttaat
    10021 caagccaaaa caatagcagt tgttcataat gggataatag aaaactatat ggaaataaaa
    10081 gaagaattaa tttctgaagg tgttaagttt gaatctcaaa cagatacaga ggtaatagca
    10141 catttagtag ataaatacta tgagggtaat ttattagatg ctgtatacaa aactatatca
    10201 aaattaagag gagcttatgc attaggtgtt atatgtaaag aacatggaaa tgaattagtt
    10261 gcagtaagaa aagatagtcc tcttgtagtt ggtgttggtg aaggtgaaaa ctttatagca
    10321 tcagatatac ctgcattatt aaaatatact agagatgttt atttcttaga aaatggagaa
    10381 gtagttcatc taaaagacga aaatgtaacc gtttatgact ctaatagaaa tcttgttgaa
    10441 aaagaagtat ttcatgtaac ttgggatgta gaagctgcgt ctaaaggtgg atatgactac
    10501 tttatgtcaa aagaaattca tgagcaacca acaggagtaa gagaaacttt agaaagaaga
    10561 cttgatgata atggaaatat aattctagac agtataaata tttctaaaga agacttagaa
    10621 aaaataaata aagtttatat agtggcttgt ggaacagcat ataatgctgg attacttggt
    10681 aagtatgcca tagaaaaatt tgtaaatata ccagtaataa ctgatatagc atcagaattt
    10741 agatatagcg acccatttgt agatgaaaat tcattagtaa tattagttag tcaatctggt
    10801 gagacagcag atactctagc agtattaaga gattcaaagg ctaaaggagc aagaatactt
    10861 tcaataacta acgttgtagg ttcatctata gcaagagaat cagatgatgt attctatact
    10921 tgggcaggac ctgaagtagc agttgcatca actaaggcat atactactca aataacttct
    10981 ttatacatga tagctttaga ctttgctatc aaaaaaggaa caataactag agaattttat
    11041 gatagtatga tttctaagat gaaagaaata ccatcaaaaa tacaagaaat actagacaat
    11101 gaagaatata taaaagaagt tgctaaaaca gttgttagtt ctgaacatgc attctattta
    11161 ggaagaggta tagactatag cttagcaatg gaaggttcat tgaaattaaa agagatttca
    11221 tatatacatg cagaagcttt tgcagcagga gagctaaagc atggaacaat agctttaata
    11281 gaaaaaggaa ctcctgtaat tgccatagct actcaagaaa agttatttga aaaaatggtt
    11341 tctaatatgg aagaagtaag agcaagagga gcttatgtag tagcaatagc tcaaagccat
    11401 aataaggatg ttgagaaagc tgctgataaa ataatctata tacaaaattc agatgatatt
    11461 ttatcaccaa tattagcagt tgtaccaatg caattacttg cttatcatgt ctctgtacta
    11521 agaggatgcg atgtagataa gccaagaaat ctagctaaat cagtaacagt tgaataaaaa
    11581 cagattaaaa attaaataga taatatttat tgaaaagaac tcatcctgtt atttgtattt
    11641 tataggatga gttttttaga taaatattaa atttgttttg tttttgctat aataaatttt
    11701 ataaaaagat tgatattaaa tcatctgtaa aatgataaat atatagacca taccaggtat
    11761 agggaacgat aaatatagtt catataaaat atagtgagta cttaataaac atatagtcaa
    11821 tatcagataa aaaaaataat agcttatgag aaaaattaaa atacaaggta ttagtaacct
    11881 tgtgataaga taaagtgagt agtttaaaat aagaatataa ggaactattt ataaaagaat
    11941 ataatgaagc taatagagta taagaaaatt actttatttt agtaaaataa tttcaaataa
    12001 aaattttttt aaattgggag tataaattat gacaaatgat tttaaaaaag aaaagaaaga
    12061 agattatttt gttaatctta aggcaatatc tactgaagtt ttacaagaaa aagtgcaaga
    12121 taacgcttgg gttatagttg atactcgctt aaatgatgct tataatggct ggaagttaga
    12181 tggagtaaaa agaggtggac atataaaagg agcagtagat ttttctgcta attggttaag
    12241 tgtatactta gatagaaaag atgaagtgtt agaacaagca ttgaaaacta aaagaattga
    12301 tttagataaa aatatagtat tgtatgatgc aaatggaaaa gatgctttgg ttgtagcaga
    12361 ttatttatct aaaaaaggat ataaatatct atataaatat gatattaaac aatgggcaga
    12421 cgacgaaaat cttccaatgg aaagatataa aaattatcaa atgatagttc ctgcttttat
    12481 aataaaagat atattagatg gaaaaattcc tgagacattt gaagattcaa agaatataaa
    12541 aatgattgaa gcgagttggg gagaagaatc gtatacaaaa gggcatatac ctacaagtgt
    12601 tcatgtaaat actgatatta tagaacctcc tccaacatgg atgcttgata atgatgataa
    12661 cttaactaag tttgctttag actatggatt aacaaaggat gatactgtaa tagtttcaag
    12721 tagtacacct atggcatctt atagactagc tgttatattg agatatatag gagtaaaaga
    12781 tgtaagagtt ttgaatggtg gaacaaactc atggttatca gcaggatatg aattagaatt
    12841 tattagtaat ccaaaacatt catgtactaa ttttggagca gatataccag ttaactcaca
    12901 attagttgtt actacatctg aattaagaca aaaactaaaa gaaaaaaata agtttatact
    12961 agtagataac cgtacttggg atgaacatat aggaaaagta tctggatata catattatga
    13021 taaaaaaggg cgtataccag gagctttata tggacattca ggtagtgata gtgtttcact
    13081 agaagaatat cgtaatattg ataatacaat gagaaataaa tatgagattt tagaaatgtg
    13141 ggacaaggaa aatatagatg tgaataaaca gcttatattt atgtgtggaa gtggatggcg
    13201 tgctgctgaa gtattaacat atgcaaatgt cataggagtg gaaaatacct ccctttatag
    13261 tgatggatgg atgggctgga gtcttgataa ttcaaattta attgaagtgg gagaacacaa
    13321 ataaagtttt tgaaagtaca atagagttgt ttaaaacaaa ggtgtttttg atttattagg
    13381 aagagtgtgt aaattgtgta ttgggaagtg gacttaggta tgttcatttg gagctatgga
    13441 aactgataaa aaagtattat ctgtttattt tgctggtaaa atatgaatag attatagatt
    13501 atagattata gattatagat tatagattat agattataga ttatagattt aagtatagac
    13561 taagtaattt atattacaga agagaatata taagaattaa tgcaagatgt atttgataca
    13621 tataagaaat tggcaagtcc aggagaagga tagagaaaat tgagattgaa gagtttaaaa
    13681 gagattatta gatgaaaaat acaaaaatgt ataaaaatac atattgagaa agtatatctc
    13741 tcttaaattt gttaataatt ctattataca gaaaaacata tttaaggggg attttttact
    13801 atgaaaaaag taaaattagc tataagcttt tttatat
//
LOCUS       DAEMIK010000121        13658 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEMIK010000121 DAEMIK010000000
VERSION     DAEMIK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.122"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..497
                     /locus_tag="KPK87_002865"
     CDS             99..497
                     /locus_tag="KPK87_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538997.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(620..1636)
                     /gene="ccpA"
                     /locus_tag="KPK87_002866"
     CDS             complement(620..1636)
                     /gene="ccpA"
                     /locus_tag="KPK87_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG2538998.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(1889..2089)
                     /locus_tag="KPK87_002867"
     CDS             complement(1889..2089)
                     /locus_tag="KPK87_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2538999.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(2123..2302)
                     /locus_tag="KPK87_002868"
     CDS             complement(2123..2302)
                     /locus_tag="KPK87_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539000.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(2484..2690)
                     /locus_tag="KPK87_002869"
     CDS             complement(2484..2690)
                     /locus_tag="KPK87_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539001.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(2738..3163)
                     /locus_tag="KPK87_002870"
     CDS             complement(2738..3163)
                     /locus_tag="KPK87_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539002.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            3335..3562
                     /gene="acpP"
                     /locus_tag="KPK87_002871"
     CDS             3335..3562
                     /gene="acpP"
                     /locus_tag="KPK87_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG2539003.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(3706..4710)
                     /locus_tag="KPK87_002872"
     CDS             complement(3706..4710)
                     /locus_tag="KPK87_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2539004.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVMFYEEK
                     "
     gene            complement(4724..5626)
                     /locus_tag="KPK87_002873"
     CDS             complement(4724..5626)
                     /locus_tag="KPK87_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2539005.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSIA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(5821..6996)
                     /locus_tag="KPK87_002874"
     CDS             complement(5821..6996)
                     /locus_tag="KPK87_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG2539006.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNVDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(7083..7928)
                     /locus_tag="KPK87_002875"
     CDS             complement(7083..7928)
                     /locus_tag="KPK87_002875"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG2539007.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(8026..8823)
                     /locus_tag="KPK87_002876"
     CDS             complement(8026..8823)
                     /locus_tag="KPK87_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG2539008.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(8842..9852)
                     /locus_tag="KPK87_002877"
     CDS             complement(8842..9852)
                     /locus_tag="KPK87_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2539009.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(9871..10653)
                     /locus_tag="KPK87_002878"
     CDS             complement(9871..10653)
                     /locus_tag="KPK87_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2539010.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(10712..11848)
                     /locus_tag="KPK87_002879"
     CDS             complement(10712..11848)
                     /locus_tag="KPK87_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG2539011.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(12557..13024)
                     /locus_tag="KPK87_002880"
     CDS             complement(12557..13024)
                     /locus_tag="KPK87_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG2539012.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKKGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(13103..13645)
                     /locus_tag="KPK87_002881"
     CDS             complement(13103..13645)
                     /locus_tag="KPK87_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539013.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
BASE COUNT         4272 a         2576 c         1459 g         5351 t
ORIGIN      
        1 atattatata tcactcacaa ttcaccactt caatattatt tctatatata aatagtatga
       61 atataattca atatatattt atagaaaggg gtttttttat gaaaaaatta gatacagagt
      121 cattagaaaa aatggctaaa caaaaaaata ttgacaagga taaaatagaa aagatggctg
      181 atagttacaa aggaaaatca gaaaatgaac ttatggagga acttataaaa ataggtaaga
      241 atctagatgg cagagatgaa gtagtatcaa aattcaaagc atttttagat gaaaatcaac
      301 gcaaaaaact ggataatata atggaaaaaa tctcagatgc agaaaaccaa agagatacta
      361 aaccagctaa aactaagaag gctaaaccta ctaaaggtaa gtcaaattca cctgcaccaa
      421 aaaatacacc aggtagtcaa caaaaaaaat ctaaaagttt atttaagaaa actaaaaagt
      481 ctaatcctca tgaatagata aaaataaact atttaagcaa ttagataatt atttttagtt
      541 aatctattat taaatttgat attattgaca taaaaaatca ggtagtgcct aaggcactac
      601 ctgattgatt ttaatagttt tatttttttc tttctgtaac actttctcta tcaacaattc
      661 tataaggtaa ctctattttc ttattttcaa ttaattcctt atttattaat tttataacca
      721 ttcttattgc tactgctccc atatcgtata gaggttgata tactgtagta agtttaggtc
      781 tatacattct agctatctta acatcattaa atcctgcaac tgatatgtct tctggaacct
      841 tatatccagc atcacatata gcatttatag cacctatggc agcctcatca cctgttacaa
      901 aagccgcatg aggtattatt ccatcatcta atagttcttt catactattg tatccactct
      961 cataatcagt tccaccatac ttaatcaaac tcttgtctaa ttctatattg ttatttgaaa
     1021 gtgctttctc ataaccagca agtctttcca tttctaaaac agtatcatct ttactagtca
     1081 ttataaaagc tatcttttca tgacctttct ttataagatg ctctgtcata tcaaaagtag
     1141 cttcttcgtt gctagtactt actgtatata tatcataatt tctagcagtt ttacttatat
     1201 atgtcgctgg tattccacaa ttttgtatat agttgatatg ttcttgttca actatccatg
     1261 aaatcataac tataccttcc acttgttttg ctctcaatag atttatactc ttaagttctt
     1321 gttccttatc agagtatgta ttcgctaaaa gaatatcata gtcatacatt ttagcaacct
     1381 cttctatccc atttaacacc tcattaacaa aagaatctga aacttctgga actattaccc
     1441 ctattaattg actcttcttt gttactaagc ttctagcaag tggatttggt atatatccag
     1501 tctcttttat aacctctaaa actttttgtt tgacttcatc agttacaggt tttgaatcat
     1561 ttataactct agatacagta gatattgaca ctcctgcttg tttagcaaca tcttttatcg
     1621 ttatattgcc tttcatcttc atcctcctta ttttactaaa tttatctaaa actgaaccaa
     1681 atttttgaag gttcctattt taactacatt tatataagtt tttattttac cataaaagca
     1741 agtacattta aatacattat attttatatt ataccctata ttcaacaaat ttaataatat
     1801 tcattcttaa tttaaatcat attaaaaaat aaaataaaaa aatgctggta taaaatacca
     1861 gcattttttt attctaatga tttaagtatt agcagccgca acctacttca ccatcaaaga
     1921 atccaccaca tgaacaagtt attagcaata atattaagaa aggtatccat gacattatgt
     1981 caatgtctct ccagcaatct tcaaaagcta agaataggaa gaatagtact attatccacc
     2041 atcctcctcc accaaagcat ccttcttctc caaaaaatct atctaacata acaaagccct
     2101 ccttttttta ataactgtgt tcttattcta agcatccacc acagcagcaa agtattaata
     2161 aaactattag gaatggtatc catgccatta tgtcaaattc tgcccagcta tctccaaaag
     2221 ctaagaatag gaagaatagt actattatcc accatcctcc tccaccaaaa catccttctt
     2281 ctccaaagaa tctatctaac attatacgtt acctcctgtt gatattttgt tgagcttttc
     2341 gctcctaaca tatactatgc atttggtttc cattttgcta atgtattttt actatttttt
     2401 taaatttttt aatttgaact aaaaaaggta aaaccataac ttgtataagt tactggtttt
     2461 acctatttaa taatcaatat tttttagcaa caacaaccat catcgaagtt aaataataac
     2521 aatactatta atataaccca tatccatata ttgtcatcgc ataataagtc ttctaaaatg
     2581 tctgtgcata atagtaagaa tactataact attattatca acattttatt cccaaaaaat
     2641 ccttcgcatc ctccaaagaa acgttccata ttgtcttcta attttgtcat aactaatcct
     2701 ccttgaatca ttaagagact aagttttgtc tctttaatta aatatatgta gtaatagaca
     2761 gataagttac ttctaaaaag tttttattga ttagtttatt tatttctttt tcaccaagtt
     2821 tattgtatct atcaacttca tccagataat cttttttctc ctctgatata gtatagatta
     2881 aactttctat tgattttatt ttgtactcct tatatctttc tatcaagaag gatgtttttg
     2941 aattttctat tcctttcttt acatgcatta accttgatat gtaatcttct ttttcattag
     3001 atacataact ctccttttca gctacatctt caaggattaa attggtctcc attacataat
     3061 cctctatacg ttgcatatct aaagttattg aattataata aaaatttact gctataagta
     3121 aaaacattcc tactgaagtt agtaatagtc ctacatattt catagcattt cctcctaagt
     3181 tatatttgtt atcaataaag tttattcatc aaataatact ttattactac atttataaaa
     3241 ttttattatt ttttatagat tccatattga aaaattcttg atattatatt aaactaacat
     3301 taaaattaat taaaactgct ttaggaggct taaaatgttt gagaaaataa aagaaataat
     3361 agcagaacaa ttaggggtag acaatttaga tgagataact atggaagcat ctttaatgga
     3421 cgacttagaa gctgattcat tagatgctgt tgaaattata atggcccttg aagatgaatt
     3481 tggaatagaa ataccagacg aggaagctga aaactttaaa tgtattggtg atatatgtaa
     3541 atatatcgaa gaaaacaaat agtatcgttt tgtctacaaa tataaatagt ctgtgtaaac
     3601 ttctacataa taaattatat tagagttcgc acagactaga ttacatttat ttacaatata
     3661 taaaatacac gctcttatat ttaatttata tactaagtat ataaattact tttcttcata
     3721 aaacataaca ttctttatta ttatatctat agttccatct tgattttgtt ttacagtata
     3781 cttcattggg tcttcaaaat cagttagatt cccttttatg tcaaaaccat tatccgtttt
     3841 tatacttctt tttttaagct ttttctcaac ccatttttta tctatactaa aactttcgtc
     3901 aagacctttt tcttccatat gctctttaaa gctatccttt aactcatcat ctttaattga
     3961 attatcaaca aagtcattta tatcaacttc atgtttttcc ttcagtgtat aatttaatat
     4021 acttcttaca tcttcagctt gctttatatc attactaaga gcattggtta tccaattttc
     4081 agctgtattt ttaaatacct tggtcttgta cttatcatca tctatttttt tagcatttaa
     4141 aaattctgtt acaaacttag attctgaccc ttctttttca gcatccttat ctaaaagcct
     4201 tagatgaaac tcatcattta taccacttag actaatcaaa gctccttgct tttgtcttaa
     4261 agtctctggg ataccaattt catttgaaac catttgaata ttaaatttat catccacaaa
     4321 ttcaattgaa tgagtgtaca actttttata atctaatttc agtattgcta cactcttttc
     4381 atctttaata gtgtataaac aaattgccaa atcacaagat tctagtgcag cattaatttt
     4441 cataacgtca aacaagtaag atgcaatttc tttagaattt ttcaaaaaag tactttcatc
     4501 atatattatt tgttcacaac agtttttaat taaattgtca ttataatctt taaaaacacc
     4561 ttttcttaaa tcctcatctt ttgcaattct ttttatagct ttctggaaaa aaccatccac
     4621 ttcttgatta actttgcctt caaagtcatt taatattgga acatcactat ttttgtccaa
     4681 aacatgtatt ataaatttat gtattatcat atatatcacc ccattatctt aatttcgata
     4741 ttaactcttt catatcctct ggtatatctg ctttaaattt caatttttct tttgttcttg
     4801 gttgttcaaa ctctagctta tatgcatgaa gtgcttgcct atttattagc tcttcatcta
     4861 cataaccata caactcatca cctattattc catgacctat atgagccata tgtactctta
     4921 tttgatgtgt tctaccagtt tctaagctta cttctaatac agttgcattt tctagtcttt
     4981 ctattacttt atagtgagta acagatgact gtcctctttc atctattatc ctctttattg
     5041 aatcttctgt aggtctataa attggctcat ctatagttcc ctcattttct tttataattc
     5101 catcaactac tgttatatac tttttttcaa ctttattttc actcattgct atagacaaag
     5161 tatgatgtgc ataagcattt tttgctacta taactaaccc tgaagtattc atatctaacc
     5221 tatttacaaa tcttattttt acgttttctt tttgattatc tatatagtaa ctaataccat
     5281 ttgctattgt tttatcatag tggctttttg ttgggtgaac taccataaac ggaggtttat
     5341 tcaccataat tatatcaaaa tcatcataaa ttatctgtaa atttaaatct tgaggttcaa
     5401 aatttgcttt ttcctcgtct atttttactt ctattaaatc accactatac acctttaaac
     5461 tcggtttttt atatactcca tttactaaaa cactcttttc cctcttcatt tttgataatg
     5521 acctaactga gaaatttagc ttgtccaata aaacttcttt taaagtcatt tcttcttcat
     5581 ttgtatacga tataagatta tatctttgat tctctttttt aaacaaactt ttacctactt
     5641 tcttttatat cttcctactt tttatatctt caaaatcatg atattatata ttttacacta
     5701 tttttgttta atagataata attattttta actataaaaa cctgtaagaa taacaaatag
     5761 ttattcttac aggttaaatt cataattaaa tatttaattt gtaaatatta taatctaaaa
     5821 ctatctctta actattaaag tagttcccat tcctccacct atacaaagtg tagcaagacc
     5881 agttttagca tctcttctct tcatttcata taaaagtgta gtaagtattc ttgctcctga
     5941 gcatcctatt ggatgtccta tagctattgc tccaccatta acattaactt tgttcatatc
     6001 tacatttaag tctcttatta cagctacaga ttgggcagca aatgcctcat tagcttcaac
     6061 taaatctata tcttcaatag tcatattagc agcttctaaa gcttttttag ttgctggaac
     6121 tggtccatat cccattattt tagggtcaac accagctgtt ccataagaaa ctatagttgc
     6181 aagaggctct attcctagtt cttcagcttt ttctttagcc attactacta gcatagcagc
     6241 accatcattt attcctgatg cattaccagc agtaactgtt ccatcttttt taaatgcagg
     6301 tcttaactta gcaagtttct ccattgtagt gccaggctta atatattcat ctttatctac
     6361 tacagtgtca ccttttcttc cttttataac aacaggaact atttcttcat caaattttcc
     6421 ttcagcttga gctttttcag ctttattttg acttgcaaga gctaattcat cttgttcttc
     6481 tctagttata ttccattgct ctgctatgtt ttcagcagta atacccatgt gatagttatt
     6541 aaatatgtct gataatccat cttttatcat tgaatcaaca aaagcagcat cacccattct
     6601 tgcaccatat ctcgcacttg gtactaaata aggagacata ctcatgtttt cagctccacc
     6661 aactaacatt atatcagcat cacctaatgc tataagttga gatgccattg aaacagatct
     6721 taatccagaa ccacaaacta tatttatagt catagctggt ttttctactg gtattcctgc
     6781 tcctaatgct atttgtcttg ctatattttg tccaagacct gctgtaagta ctccccctaa
     6841 aagagattca tctatcatat ctggagttat gttagctctt tttatagctt ctttagctgc
     6901 tgttacccct aactctaccg ctgaaactga tttaaatgct cctccaaaac ttcctactgc
     6961 tgttctagct gcactggcaa ttactacttc tctcatattc attaccccca ttaaattaga
     7021 tttttcatat atccgaaatt gttttttaaa caattttatt taaactttta attcccaaat
     7081 tcttatttat tataatcata gaatcctatc ttagtttttc ttcctaattg attagctcta
     7141 accattttag ctaaaagtgg atgaggtcta tatttagtat ctccaaactc agtatataaa
     7201 acgttcatta tagctaaaac aacatctaat ccaattaaat cacctaatgc tagtggtccc
     7261 attggatggt ttgctcctaa tttcatagct tcatctattt cttctttact tgcaacacca
     7321 tctgcatata taccaacagc ttcatttatc ataggtataa gtattctatt tactacaaat
     7381 ccaggagatt cagatacatc tactggtact ttattgatac tcttagataa ttcaaatact
     7441 gtatcaaaag taacttttga tgttaactga ccacttataa cttcaactaa tttcatcata
     7501 ggaactggat taaagaaatg cattcctata actttatctg gtctcttagt agaagaagct
     7561 atttctgtta tagataatga tgaagtattt gttgccaaga tagtatcttc tttacataat
     7621 tcatctagta acttgaaaac atctttcttt atattcatgt cttctacaga tgcttctatt
     7681 attaaatcca tatcttttaa atcttcatag ttagtagttg aactaacatg acttaatatt
     7741 tctgcttttg tagcttcatc catttttccc ttagtaacta acttagttaa atttttatct
     7801 aataaagcta aacatttatc tatagcacct tgagttctac tctttaaaca tacatcatgt
     7861 ccacaacttg caaaagtttg tacaatacca cttcccatag ttccactacc tattacagct
     7921 aatttcatag ttataacctc ctgttatttt agtatttata ttctgttaac tagattaaaa
     7981 tactatttaa tctagttaac agcaaatata tttttaatta gatttttacc cttttataaa
     8041 gttagcttct ctcttttcaa cgaaagctga cattccttct ttttggtctt tagttgaaaa
     8101 acaaagacca aataaattag attctatatc tattccagta tttatatcag tttgagcacc
     8161 aagttgtatt gcttctttag agtatctaac tgcaagctga gcatttttag ctattatctt
     8221 agctaatttc tcaacttctt ctattaaaat gtctggctca acgactctat ttactagccc
     8281 tattttttca gcttcatcag cttttataac ttgacctgta aagattaatt cttttgcttt
     8341 tgccattcca accaatcttg taagcctttg agttcctcca tatcctggag ttattccaag
     8401 agttacttct ggctgaccaa atttagcttt agcagatgca attcttatat cacatgccat
     8461 tgcaagttca catcctccac ctaaagcaaa tccgtttaca gcagctatca ctactttttt
     8521 actattttct atttctccaa aagcttttgc tcctaagata ctaaaatctt tagcagctac
     8581 agcatctaaa tctttcatgt atgcaatatc tgctccagct acaaatgcct ttccttcccc
     8641 tgtcaatatt acaacatcaa tagtttcatc attattaata tctacaaata cttcataaag
     8701 ttcttctaaa gtctttgaat ttattgcatt aagggcttta ggtctattca ttttcactgt
     8761 acatatattt ccatctactt caacagctac attctcataa actttaacat cacttgtact
     8821 cataataccc tccccaagaa attagttagc taaaacttca cctttttctt ttgcaacact
     8881 taactgactg ataagttctg gaagcacttt atgaacatct ccaactatac caacatcagc
     8941 atatttaaat attggagctt ctggattttt atttatagca actataaact cagcatcttc
     9001 cataccagct atatgttgta ttgctccaga tataccacat gctatataaa ggtctggtct
     9061 tacagtttta ccagtttgac caacttgtct tgctttatct aaccaacctg catctatagt
     9121 ggcacgagaa ccagaaactt ctccacctat aatttcagct aattcataaa gtatgtctaa
     9181 gttttctttt ccacccattc cacgtccagc agaaactaat atcttagcat cttctatttt
     9241 aacttgtttt ttagcttctt ttattacttg tacaacttga actaatttat cagcatcatt
     9301 aaattctacc ttgaaacggt taattacagc ttctttagtt tcatcaggtt catttttctt
     9361 cataacccct ggtctaactg tagacatttg aggtctgaaa tctttacaaa ctattgttgc
     9421 cattatattt ccaccaaatg caggtcttgt cattaataat aattttgtat cttcagctac
     9481 tgcaagacct gtacagtcag cagtaagacc tgtatgtatt ctagcagaaa ctctaggagc
     9541 taaatctcta cctattgaag ttgcaccaaa taatacaact atagggtcag ctgcttttat
     9601 tgcttcataa gctgcttttg tatatggttc agttgtatac actgctaaag cttcatcatc
     9661 tactactatt acctcatctg caccatagtg tgctaatgta tctattaaac cttctacctt
     9721 actacctaaa agtaatgcag aaacttttgt atcataatct tttgctattt ctgtagcctt
     9781 tcctagtaat tctaaagaaa cagtttgaat tacattttct ctttgttcta ttactactaa
     9841 aacgttaccc attctttatc cccccttaaa ttatatgata tacttctctt ttaatttatc
     9901 tatgataatt ccagctgatg ttttcgcatc ttcattgtat attgtaccag ctggtttaac
     9961 tgattttgta aatgatttaa atacactagt tggagaacct ttaagaccta aattagaagg
    10021 gtcaacttct atatctttta cagtccatgt ttctactaca tcattttcga aagcatcata
    10081 tattcttcca actttcatgt atcttggtgt gttcatatct ttaagagttg ttataaggca
    10141 tggcatttta acttttaagt catggcaaca atcttcaaat tgtcttttta ctaatacata
    10201 ttcaccttca gtttttattt cttcagcata tgttattgat ggaagattta aatgttcagc
    10261 tatttgaggt ccaacttgtg cagtatctcc atctatcgcc tgtcttccag ctattataat
    10321 atcaaaatct atatttttta atgctcctgc taatgctgat gaagttgccc aagtatcagc
    10381 acctgcaaat gctctatctg ttaataatat acctctatct gcacccattg ctaaagcttc
    10441 ttttaaagcc atatctgctt gaggaggtcc cattgttata acagttacat gagcacccat
    10501 ttcttctttt aattttatag cttcttctaa acctgcttta tcatcagggt ttattatact
    10561 tggtactcca tctctaatta aagtacctgt attaggatct agtttaactt ctgttgtatc
    10621 tggaacttgt tttatacaaa cgactatatt cataaatcct tcctccatat ctattttaat
    10681 atcgacctat tgtgtatatt ttaatattta tctattttaa taattttcct gaaataacca
    10741 ttctttgaac ttcactagtt ccttcatata tttcagttat cttagcatct ctcatcattc
    10801 tttctactgg atagtcacga gtgtatccat atcctccatg aagttgtaca gcttttgtag
    10861 taacttccat agctgtttca gctgcaaata attttgccat tgctgcttct actccataag
    10921 gttttcctaa gtctttattt atagctgctt gatatacaag gtgtctagcc gcttgtacct
    10981 taacttccat atcagctaat tggaattgtg tattttggaa ttttgataat ggtctaccaa
    11041 attgtactct ttcttttaca tatttaacag tttcatcaag agcaccttgt gctaaaccta
    11101 aagcttgtgc agctatacca attctaccac catcaagagt agacattgct atcttaaatc
    11161 cttgaccttc ttttccaagt aaattttctt taggtattct gcaatcctca aatattaatt
    11221 cactcgtagc tgaacctctt atacccattt tcttttcttt aactccaaag ctaaacccag
    11281 gagttccttt ttcaactata aatgctgata ttcctttgtt ccccttagat ttatcagtca
    11341 ttgccattac tacatatatg tcaccagcta ttgcgtttgt tataaatatt tttgagccat
    11401 taagtatgta ttcatccccg tctaaaacag cagttgtttg ttggccagac gcatctgtac
    11461 cagcattagg ctcagtaaga ccaaatgctc ctaatttttc tccacttgct agtggtctta
    11521 agaatttttg tttttgttct tcattaccat attgatatat aggccatgag ccaagagatg
    11581 tatgagctga taatataact cctgtagtac cacaaactct agacaattct tcaactgcca
    11641 ttatatatcc tacagtgtct ccaccttctc caccatattc ttttggatat ggtataccca
    11701 tcattcctgc ttttgccatt ttttccactg tttcataagg aaatctttct tcttcatcaa
    11761 gttctgttgc taaaggttta acttcatttt cagcgaagct tacatatagc tctttaagca
    11821 tctgatattt tttagaattt aaatccataa tagcctcctc agtattttat aaatatttta
    11881 ttttcattta ttaattaaaa tctcggcaac atctataata aggtatttga atatacgtcg
    11941 agaaatttat aaccttaatc aaactacgct ctatatttat atgtgattat actaaccaat
    12001 tatgacatta atttaatcat aaattgttaa tttaaaatca actatactta aaaaaaatct
    12061 cgctttttgg aattttgtga aatttcaagt tagaataaga tttctttcac actggttaca
    12121 gataaaataa tttgtttacg atatttatat tagggtatat taaacattaa atattgtctg
    12181 tacatctgtt ttttagccat gtacagaaga tttttatcgt ataaaaatta ttttaataga
    12241 gctaaataat cgtattttta tctgactatt ttttattttt aaatatctta catctataat
    12301 agtatcactc aaaggataat ttttcaataa tttctaatta tttttttaac aatatttatt
    12361 acattttttc cacttttttg tcattttttt attgtttcga aaaaaaatca gaatttttta
    12421 actttatttt ggattattag tttaaattag attttcctct tagatttata aatatttaca
    12481 caaaaaaact acctctgtat agattgtata cagaggtagt tttttactaa ttcctactat
    12541 agtttactat atttttttac caattcttac aaacatctac ccatttttca cacttagaaa
    12601 acttctccat ttcttcacat gtaagttcta ttgcagagtt actactgcca catgcaggaa
    12661 atactgtatc aaatcttttc attgaatcat ctaaatagac ttttatagaa ttttttaaac
    12721 caaatggaca gactccacca atagcatgtc cagtaaactc caagacttct tctggtgtca
    12781 acattttagc cttacaatta aattcagcct tatacttctt attatcaatc ttagcatcac
    12841 cagctgtcac aattaaaatt gcatcatctc cttttttaaa tgagagtgtc ttagctattc
    12901 ttgctggaat aaccccagct gcttcagctg caagttccac agtagcacta gattgttcaa
    12961 attctaatat actgtcttcc ttgccaaatt gtttaaaata ctctcttaca ttacttattg
    13021 acattaaata tccctcctag tctgaacttt tataaatttc atattattct taattatatc
    13081 tataattgat ttatttttca aattattcca tgatattagc attccatgta tcattatttt
    13141 ttccaactac tagataaagt ccttttgcca ttggaatatt gacaatatat tctcctttat
    13201 actctacact cataccagat tcattaaagt acctataatc aaaatttagt ccttttggaa
    13261 ttacaaaatc aacataacct tgattatttg gttttattcc atatttcaga gttctttcag
    13321 ttatagaatt tttttcatta aataaatcta catttacata tgcagaacac ttattcattc
    13381 cctttttatt ttccacacaa ttttcaaact tcattttagc actgtttaat ttataactat
    13441 atttactatt attgcttatt ttaaatctta caatagtacc aacatctcta cttctcctgc
    13501 tatcaaaatc aaaaacaatt tcctctaaat gatttatttc ataatctttg acacttacca
    13561 tatctctata tactaattta tttatagcta tgaatagtgt aaaaatcccc aaaataaaaa
    13621 atgaaacttt tataaacttc ttcattttta gtctcctt
//
LOCUS       DAEMIK010000122        13268 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEMIK010000122 DAEMIK010000000
VERSION     DAEMIK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.123"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            110..463
                     /locus_tag="KPK87_002882"
     CDS             110..463
                     /locus_tag="KPK87_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539014.1"
                     /translation="MAKRPVEKYDFKAFGEAIKAARTGRKESRKQVSDEMYISPRYLA
                     NIENKGQHPSLQIFFELMLRYNISVDQFLLDTPTQKDTKRRQLDALLDDMSDNGIRIV
                     TATAKEISEVEKEDK"
     gene            712..1041
                     /gene="mobC"
                     /locus_tag="KPK87_002883"
     CDS             712..1041
                     /gene="mobC"
                     /locus_tag="KPK87_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG2539015.1"
                     /translation="MNGRKRKVQIKFYVTEEERVLIEQKMKLVPTQNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIAKRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            1002..2351
                     /locus_tag="KPK87_002884"
     CDS             1002..2351
                     /locus_tag="KPK87_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG2539016.1"
                     /translation="MAVTKIKPVKSTLSKALDYIQNPDKTDGKTLVSSFGCSYETADI
                     EFEYTLSQARQKGNNLAFHLIQSFEPGEVDCEKAHAIGKQLADAATKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGTSWKGKLKIAVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIAGKRTKAAKAPKEQRGVSLLIDIENSIKAQESRGYE
                     QWAKIHNLKLAAKTMNFLTEHQIEQYADLVSRIEEITAENEKTADALKSVEKRLADMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKNESSLILHEAAAKTLKASGVTKLPNLA
                     AMQEEYGKLQAQKEALYTDYGKLKKQVKEYDVIKQNIDSILRLEKEPERSRETERLQ"
     gene            2412..2681
                     /locus_tag="KPK87_002885"
     CDS             2412..2681
                     /locus_tag="KPK87_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539017.1"
                     /translation="MADQTIPDYETIRAAVAGETWAVEKVLMCYKDEIDRQATVKKRQ
                     PDGTFKLEIDEDMRQYITMKLIEALPQFPLEEMEREEKRNRDKKS"
     gene            2728..3021
                     /locus_tag="KPK87_002886"
     CDS             2728..3021
                     /locus_tag="KPK87_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539018.1"
                     /translation="MKQRQKETISTWLYEEYRRLWIEKGQEPHKRNNREIVAAVMEQI
                     EAAGIWLPEQEVTKYFYRRKTHYRNRIEKELQPKPEMQDKDSQTQKEQGAGSQ"
     gene            complement(3166..3531)
                     /locus_tag="KPK87_002887"
     CDS             complement(3166..3531)
                     /locus_tag="KPK87_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBG2539019.1"
                     /translation="MTGSKRLTPPQSRKVNSLVKKECCNCERGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECSTFFVSKSNSVKYCPDCRKR
                     ITRRQAAERMRKRRAAVTQ"
     gene            complement(3528..3734)
                     /locus_tag="KPK87_002888"
     CDS             complement(3528..3734)
                     /locus_tag="KPK87_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBG2539020.1"
                     /translation="MIRLTVEEMNLLSIYHEGSKAQLMENMTTALPFMDEDMRPFAER
                     TLQKISPLTENEYAELSIFAADEV"
     gene            complement(3737..4681)
                     /locus_tag="KPK87_002889"
     CDS             complement(3737..4681)
                     /locus_tag="KPK87_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539021.1"
                     /translation="MKLQKFENVDVIASLEAIMKQNTAFYQSDFDIDKQILQKAAVSP
                     VPEDKRLLWFSRPSGTCCFRERDVFLKDTRQHNTWRFYGEQTRDTILAYAVELTGTEQ
                     EKIKGNLYELDYLQHFREVIEKSIPADNYTLIYEHGELTKPAGQYFDGNTDPQLGKFI
                     RFEAQPNDPEALKSILREQQKKRTPHTQGDFQLHIAALHDRRIEAEARRIVENVKGLG
                     EPDSPEKTHCAVELSPYFVPLATDKDMERLLSMLPYKSLSFSTLEDRHGVYALIDKSE
                     RNKEMKKSRPSVRAQLQTEKSPKLPKKTAKSKHHELEV"
     gene            complement(4685..5287)
                     /locus_tag="KPK87_002890"
                     /pseudo
     CDS             complement(4685..5287)
                     /locus_tag="KPK87_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
     gene            complement(5615..5821)
                     /locus_tag="KPK87_002891"
                     /pseudo
     CDS             complement(5615..5821)
                     /locus_tag="KPK87_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6803..9070)
                     /locus_tag="KPK87_002892"
                     /pseudo
     CDS             complement(6803..9070)
                     /locus_tag="KPK87_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            complement(9312..11402)
                     /locus_tag="KPK87_002893"
     CDS             complement(9312..11402)
                     /locus_tag="KPK87_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG2539022.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWSYDSLPILPQEWKYTVAADKEKQFKTLKELIHRADVSEVVNAC
                     YAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGEEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASERISDKAEADALKTTCEASRTVCTSLVKEKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSAFLTDDMGDTAAGIIKLLCGKFSFMAGKD
                     FTPELAKVLNSKKVSDHHAIIPTMELSKTDLAALPESERNILTLAGARLLMATAAPHT
                     FEAVTAIFECAGQSFTARGKRVLSDGWKELDRRYRAALKNKPETDDADSDTEKTLPPF
                     TEGQTFENSAATVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKAEP
                     AAFMEGIEDMARELVKTYPFLSEADKGRFKEEKPELGKCPRCGSPVYEGKKNYYCSNK
                     ECIFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIVLADTG
                     GKYVNYRIELPKKK"
     gene            complement(11523..11843)
                     /locus_tag="KPK87_002894"
     CDS             complement(11523..11843)
                     /locus_tag="KPK87_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HBG2539023.1"
                     /translation="MASSKEYLEFILGQLSELEEITYRAMIGEFIIYYRGKIVGGIYD
                     DRLLVKPVKSAISYMPTAPYELPYEGAKEMLLVDEVDNKEFLTGLFHAMYDELPAPKP
                     KKKK"
     gene            complement(12112..12840)
                     /locus_tag="KPK87_002895"
     CDS             complement(12112..12840)
                     /locus_tag="KPK87_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG2539024.1"
                     /translation="MKNKLKKTLTALCAALILMGGFSVPAYAQGAATEPPAEDATNDS
                     GVIVEEKEETPPLTPKGNATLVDDYGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEAAKKPESCNCTEKCEAGKVNTSCPVCVTSMTGCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFLKPKQNVKGDTDLEDFEFED
                     YDEDEPETDTGETPEDEETEEETL"
     gene            complement(12830..13099)
                     /locus_tag="KPK87_002896"
     CDS             complement(12830..13099)
                     /locus_tag="KPK87_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBG2539025.1"
                     /translation="MANNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAPATEAADYEEQEENADEE"
BASE COUNT         3104 a         3261 c         3220 g         3683 t
ORIGIN      
        1 acgaacaata gcaagtatat aaatatgaat tattatggct atttagtgga actgtataga
       61 catatccaaa gagaaagggg aacagtaaaa tgtaactggg tgaataaata tggcaaaaag
      121 accggtagaa aaatatgact tcaaggcatt tggagaagct ataaaagctg cgcggacggg
      181 gcgcaaagaa agccgcaaac aggtaagtga tgaaatgtat atttctcctc gctatcttgc
      241 aaatatcgaa aacaaaggac agcaccccag tctgcaaatc ttttttgagc ttatgcttcg
      301 ttacaatata tccgtagacc agtttctttt agatacgccc acccaaaaag atacaaagcg
      361 gcggcagctc gacgctctcc ttgatgatat gagcgataat ggcatacgga ttgtaacggc
      421 aactgcaaaa gaaatttcag aagtcgaaaa agaggacaaa taaatgtgtc cggcaaattg
      481 aatatgattt aggaagcgtt gtaatcttta cagattgcag cgtttttctt ttgctctttt
      541 ttggcatata gagcattgcg ttgtagtaac cagtgaggaa aaaactttcg tagcaagcat
      601 aggaaaagag tacccttttc cgtattttcg ccctcccgtc ctgcggtgcg tcggttgaaa
      661 attgcttgtt gggaagtgtc ccaaaccctc aaagcggaaa ggagcgattg catgaacgga
      721 cgcaaaagaa aagtacaaat caaattctat gtaacagagg aagaaagggt gttgattgag
      781 cagaaaatga agctcgtccc tacccaaaac atggcggcgt atcttcgcaa gattgccatt
      841 gacggctata tcattcagac agaccatagc gatataaaag ccatgacagc ggagatacag
      901 aaaatcgggg tcaacatcaa tcagattgca aagcgtgtca atgcgacagg cagcgtctac
      961 caagaggaca tagaggaaat aaagggggtg cttgcagaga tatggcggtt acaaagatta
     1021 agcctgttaa aagcacgttg agcaaagccc ttgattatat ccaaaatccg gataagacag
     1081 acgggaaaac gcttgtgtcc tcttttgggt gcagctatga aacggcagat attgagtttg
     1141 aatatacctt gtcgcaggct cggcagaaag ggaacaactt agcctttcat ctgatacaat
     1201 cgtttgaacc gggagaggtg gactgtgaga aagcccatgc aatcggaaag cagcttgccg
     1261 acgcagcaac aaagggacag cacgaatacg tccttaccac ccacatagac aaggggcata
     1321 tccataacca catcattttt tgcgcggtca actttgttga ctaccataag tacaattcca
     1381 ataagcgcag ctactacggc atacggaata tgagcgacaa gctgtgtcgg gaaaacgggc
     1441 tgtccgtcgt cgttccgggc aaaggcagca agggaaaaag ctatgcagaa tatcaagcgg
     1501 aaaaggtcgg tacaagttgg aaaggcaagc taaaaattgc cgttgatacg cttatccccc
     1561 aagtagcaag ttttgaagaa ttgttgcagc ggttacaggc agcgggctat gagataaagc
     1621 cgggaaaata tatctcctgc cgcgctcccg gacaggaacg gtttacccgc ttaaaaaccc
     1681 tcggtgcaga ttatacagag gaagcaatca aagaacggat tgcaggcaag cgcacaaagg
     1741 cagcgaaagc tcccaaagag cagcgtggcg tatcgctgct tatcgacatt gaaaacagta
     1801 tcaaggcaca agagagccgg ggctatgaac agtgggcgaa aatccataat ctgaaactgg
     1861 cggcaaagac catgaatttc ttgacagaac atcagattga gcagtacgcg gatttagtca
     1921 gccggattga agaaataact gcggaaaacg aaaagaccgc agacgcatta aagagtgtgg
     1981 aaaagcgtct tgcagatatg gcggtgctga tgaagcacgt taccacctat caaaagacaa
     2041 aaccagtcta tgaagcatac cgcagggcaa aggataaaga cgcataccgg gcaaaaaatg
     2101 aaagcagtct gatactccat gaagcggcgg caaagacact gaaagcgtcg ggcgttacca
     2161 agctccccaa tctcgccgcc atgcaggaag aatacgggaa gctccaagcg cagaaagaag
     2221 ccctttacac tgattatggc aagctgaaaa aacaggtcaa ggaatacgac gttatcaaac
     2281 agaacataga cagtatttta agactggaaa aagaaccgga acggagcagg gaaaccgaac
     2341 gcttacagta gaaatatcta taaaagcgtg gtaggataaa gatattccaa tacagacagg
     2401 agcgtgaaaa catggcagac caaacaatac ccgattatga aaccatacgc gccgccgttg
     2461 ccggagaaac atgggcggtg gaaaaggtgc ttatgtgcta caaggacgaa atcgacaggc
     2521 aggcaactgt aaagaaacgt cagccggacg gaacattcaa actggaaatt gatgaagata
     2581 tgcgccagta tatcacaatg aagctgatag aagccctccc ccaatttccc cttgaagaaa
     2641 tggaaagaga ggaaaaaaga aatcgggaca aaaaatcata aaaaggtaga cgggaagctg
     2701 ctgcggatag ataagcagtt tcgacatttg aagcaacggc aaaaagaaac tatcagtaca
     2761 tggctttatg aggaataccg ccgcctttgg atagaaaagg gacaggagcc gcataaacgc
     2821 aacaaccggg aaatcgttgc cgctgttatg gaacagatag aagctgcggg gatatggctg
     2881 ccggaacagg aagtaacaaa atatttttac cgcaggaaaa cccattaccg gaaccggata
     2941 gaaaaagaat tacagccgaa gccggaaatg caggataaag acagtcagac acaaaaagaa
     3001 cagggtgcag gaagccagta atgacctccc gcaccctgtt cttttatagg tactgatttt
     3061 agaatgttac gcagtagaac cccgatttca agggaaaagg tattctgtat cgcctatgcc
     3121 agtttcacgc cgtttatgcc ctgtaaatca aggctttttt cgcccttatt gcgtaaccgc
     3181 cgcgcgtcgt ttcctcatgc gttccgctgc ctgtctgcgg gtaatccgtt tccggcagtc
     3241 cgggcagtat ttgacgctgt ttgattttga tacaaagaac gtgctgcact cggtacaacg
     3301 ctttttatcc tcggtcttga aaagctctgc atagagcaac ttatcaaggg gaagtacggc
     3361 ggtctgaaac cacttgcata gaagtgaata ggaaataagc tgcgggcaga cacattcctc
     3421 cccgtcgtcc aataagatac agtgtccgcg ctcgcagttg cagcactcct tttttacaag
     3481 gctgttgact ttccggctct gtggcggcgt gagccgctta ctcccggtca tacttcatca
     3541 gcggcgaaaa tggaaagctc cgcatactcg ttttctgtta gtggggaaat tttctgtaag
     3601 gtgcgttctg caaagggacg catatcctcg tccatgaatg gtagcgcagt cgtcatattt
     3661 tccataagct gtgccttgct tccctcatgg taaatactca acaggttcat ttcttcaacg
     3721 gtcagtctaa tcatgctcat acctccaatt cgtgatgttt tgattttgct gtctttttcg
     3781 ggagttttgg ggatttttct gtttgtaact gcgcccggac agagggacgg gactttttca
     3841 tctctttatt cctctcgctt ttatctatca gcgcataaac gccatgcctg tcctccaacg
     3901 tagaaaagga aaggcttttg taagggagca tggaaagcag ccgttccata tccttatccg
     3961 ttgccagtgg aacaaagtag ggagaaagtt ctaccgcaca atgggtcttt tcggggctgt
     4021 ccggttctcc aagccctttt acattttcca caatccggcg ggcttctgct tcaatccgtc
     4081 tgtcatgcag ggcggcgata tgtaactgaa aatccccctg cgtatgcggt gttcgttttt
     4141 tctgctgttc cctcaaaatg ctttttaagg cttccgggtc gtttggctgt gcttcaaaac
     4201 gtataaactt tccaagctgc gggtcagtgt tcccgtcaaa atattgtccg gcaggttttg
     4261 tcagctcccc atgctcataa atcagcgtgt agttgtcggc agggattgat ttttcgatta
     4321 cttcccggaa atgctgcaaa taatccagtt cataaagatt gcccttgatt ttttcctgct
     4381 ctgtgcctgt cagttccacc gcataggcaa ggatagtatc gcgggtctgt tccccgtaaa
     4441 accgccatgt attatgctgc cttgtgtcct ttagaaaaac gtcccgttcc cggaagcagc
     4501 acgtcccgga cgggcgggaa aaccacaaaa gccgtttgtc ctccgggaca gggcttaccg
     4561 ccgctttctg caaaatctgt ttgtctatgt caaaatcgct ctgataaaaa gcggtattct
     4621 gtttcattat ggcttcaagg gaagcgataa cgtccacatt ttcaaatttt tgtaacttca
     4681 taggtcagct cctttccaaa tcctgtgttt tctgtcttgc ctgtttcttt ggtgtctgtt
     4741 ctttcgctgc tttaagctgc gcccggatag agggcttttc ctgtttggca ggcttgccac
     4801 gttccttatc ggttttcaat gcttcggcgt attctgcaag ggaaatctgt cctccggctt
     4861 ttgctttttc ttccagttcc gcagcggtcg gggtgttgtt tatctgcccg tcaatcatgt
     4921 tgtaattttg ctcggtggtc tgctcggcag ctttcaaata gtttttcggc tgcaaaaact
     4981 ctggcacgtc cttatacccc aagctgtcta catagtgggc ggtgtcctgc ccgttctgat
     5041 gaagcaccac cacgtcggaa acggaaaggc tgtgtccctt aaaatctttc gggtggtcga
     5101 tattgaagcg ggtgtaaata tcttcaaggg acattcccgg cgtaaggggt gcggtataga
     5161 taagctcata attgcctctg tcaatgctgt ttcccgccgc ctgtatgcgg tcgtaaggct
     5221 caaagcgcaa gtcccgcgtt tctttgccgt tctttagctg ataaattccg aaagtgtcct
     5281 cgttcccata gagtaagaga gcttcccttg atggctcgct ttcccgtagt tcttgcttca
     5341 tggcgttgta ctcccgcagg gcttcccatg tttccttttc cacgccgaac aagccgcctt
     5401 tttgtgcgtg tgcctgtaaa tcctccaagg tatcaacaag cccctctgtg ccgtcctcat
     5461 agagaagata aacggacata tcctcatgga aaaggcgttc agcggcttcc tgctgaagcg
     5521 gaagcatacc gtcccaagca tagccgtatt cctgcatatc cttgacgctg attgtagggt
     5581 ctggcatagg gatttccggc gcggcggtct gcgtctttgc gtaaagctcc ttgacttctc
     5641 cctgtgtcag ctccccggcg gtaagctgcg ccataagctc tcggcatttc tccggcgttc
     5701 cgatatacaa aacgtcgcct atctttgctt tcccgtcctc ctgcggaatg taggcttgca
     5761 gcagatacag gttgtccctg ctgtcgctgc ggggatttgc gtgtaccttg taaatggctt
     5821 cctgttcggg ctttttctgc gccgcttctt tttgcgcccg gatatgcgcc cgctgtatct
     5881 cggtaggctt ttccccggta aagggtgcaa tcgccttgat ataatcagcc gcatagtagg
     5941 cgttactggt aagctgccgc tgccatgccc ctgctttggg cgaccaacgg aagccattac
     6001 tttttaaggc ttcccgcgtg gcttcgtccg gcttatcttc aaagaagatt tgcagacggt
     6061 tcgcttcggt gtttgcttcc accttgccgc cgtcaaattc ccaacccaca aaaccgattt
     6121 ccttttgctg tgaaagggat ttgatacggt cttttacgcg cctgatttct gcgctgttgt
     6181 tggataacgc ccaagtcgca aaaggcttgt cccccaaatg ccaactgctc gccatgtctg
     6241 ctttcagttt ttcaagctgt tccggcgaca aatgcgggca gccgtcaagg gtcttatgct
     6301 tgcggtagta ggcgtttacg gctttcatgg tttcctgcga tttttcaagg ttttccagtt
     6361 tcttctccaa cttctgtact gcctgcgggt cgtctgcgct gataccgccc atgccggtgc
     6421 tgcggatttt atcaagaagt ccttgtatgt cctgccattc ccggtaattg ctgtcgcggg
     6481 ctgcgttctg cttttctttc ttccttgtgg ggaagttaga gccgcccgca atgaggatag
     6541 agggaacacg cgcgtcaatc tcatagcctt tgttcatgtt cgccgccagt ttccgggcgt
     6601 aagtgtcaag caggctgtcg attttctcat ggtacatcgg gtctacccgt tgtttctgcc
     6661 tttctgcaag ctgtacggct tcatctacat agtgcctgta ttcagccgtc gcgctgcctt
     6721 gcttataatc agaaaagctg ttcatatcct ttgcacgttt ggcggcagct tcattgattg
     6781 tgtaataagg tgcggcgggg gctgcctgtc ccggctgtgt agtctgcggt gcttcgctgt
     6841 ttgcgcctgt atcagcttcc ggctcggtct gtgcttcttt ttcctgtacc ggggctgttt
     6901 cttcgggaag ctccggcgcg gctgcttctg cttcgggctg tatggtagct tcctgtgggg
     6961 tctgtgcctg ttcggtggtc tgttccttat cctgtgcctt ttggatttct gcaaaatgtc
     7021 cgtctatggt atcaatcaga cttgcggcgg tgctgcggat tgtttcaaga gagcctttca
     7081 gttctgcaag ttcttttccg ctgctccacc cggcgatata cccaaaagaa tagtcggacg
     7141 tatcaagccc gtaatgttga cagaccgcat aggcaacgct ttccgcctgt acctcgcggg
     7201 tgcggcggtc gggacgttcc ggcgcgtctt tgtctatgtc atggagtgtt gcatgggcga
     7261 tttcatggat tgcggtcttg atgttctgca attcgtccat accctcgttg atagagatac
     7321 gcttttcctc ataattgcag cgtcctttta cgccgccgct tagtgcttca aatcccatag
     7381 caaacgggga agtcctttca agggcagcga aaaaatcctg atactgttcc acgtcgcccg
     7441 taagctcctt tatcccaagg tcgggcaact ccttaccctc ggtctgcgac acgtcaaaaa
     7501 cagataccac acgaaaggcg gggatttgga tttccttttc ctcggtaacg gctttcccgt
     7561 tcttatcgaa aacaggcttt tgcgtgtccg ggtcgatttt ttccacctgt ttcttaacgg
     7621 taaagggcgc gggcgcaagt atcttgatag ctttttctcc cggctttaca tggcggtcaa
     7681 attccttttc ccattgttta tagcctttta caaggttgcc gccctgcatg gcgataagga
     7741 ttgtattgtt taggctgtaa tcatggaatt tagacatagt acgcagatag tcggcgtaac
     7801 ggtcgctttc ataaatcccc aaaatgccct gttccagtcg gtcggtaatc tctttcattt
     7861 tgtcggcggg cttttcggaa gataggataa tcgggagaac cgggggctgc tctgtggcag
     7921 tggtcggtat ggctgcggca gcgtcaaggt ctgcctgttc ctgcggctgc ggcgttaccc
     7981 ggtattcctc cggcacgtcg cgcccgtcat accactgtgt aaaggtgttg cggttgttgt
     8041 agatatatcc ctgctcggta aatctgcccc cgtcattgat agcagcgtcc cgcccgtatt
     8101 cctcatacat gaaataattt tttgcttctt ccggcagctc caactcgtcc agttcttcaa
     8161 tcagataatg cccgtaatct tcctcggttt ttacggacgg ataaatccag taacagtcaa
     8221 ggttttgtgc aaggtttata atgtcctgca agtcccttgt atggtcgccg tatgtgatag
     8281 ccgcagcaaa tttttcccgg tcgtcgtcaa actgcatttt caaaagttcg ctcaaatagt
     8341 tcagttcgtc aaggctttca agctcggtca atttccctgc cagtcctgca atagaggact
     8401 ggtattctgt gatatgcagt tccccgtagt ttttgccgtc tatcccgata cggtcaaaga
     8461 cttctttcaa atgctcggca gtcgtgggga atgataccca ttcgcccgca ggtctgccct
     8521 cggtgtactt tccaagatta gaaagatacc cgcttaaaat cgtttctgcc atgcgttcac
     8581 tcctttcgtt ttcaatcatg cggtgcataa gttcgtagtc ctctatgctc atgctcccgt
     8641 caaatagata ggggtcgggt tcttcgccgt cccggtaggc tttttcagag aaaggcagcc
     8701 ccgcagcgtg ggcggcggct cttgcttcct cgataagctc cggcggcagc ctgtcgtcgt
     8761 gggcttcgat aaattccccg atattgctat atccgttttc gtagtggcga cgcaccccgg
     8821 cggtcagttc gtcaaggttc ctgtcctcgc caagatagcc ggaataatag ccgtttacca
     8881 caacggcggc agggtcagcc tgcttgattt cttccatgcg gcttctgtcc tccgggtaaa
     8941 ggtagtcgcc catttcaagg tggaaatatt cggcgttcca agaacgtccc gttttccaaa
     9001 acgccaccca agcgataccg tcccgtaatt cttcttgata gtcctttacg gtgtcccgta
     9061 aacttgccat gtgtaacctc ctttccaaag ggcagcgcgg acgctgcatg ttccggtttc
     9121 atcattcagt accccgcgcc cgcgtttttg ccaaagattt tatagatttt ttcaagattt
     9181 cagagggtaa aaccctccac aaaacagagg gtttgggaca cttcccaaca agcaaaaatc
     9241 cccgccgttc tcggtagaga aagcagggat tttacggaaa agggtactct tttcctatgc
     9301 ttgctattca gttatttttt cttcggtagt tcaatgcggt agttgacata tttcccgccc
     9361 gtatcagcca atacgatagt tccgtcgtag gttttgcccg tctttgggga atagagcttt
     9421 ttcacattta ccttgccgga ttttaagagt gcagcggcaa tctttggggt aaaggttact
     9481 tttcgttctt caaagaaacg gtcatttttc cacatggtaa agatacattc cttattactg
     9541 caatagtagt ttttctttcc ctcatagaca ggagaaccgc agcggggaca tttcccaagc
     9601 tccggctttt cctctttgaa acgccccttg tcagcttcgg aaagaaacgg ataggtcttt
     9661 accaattccc gcgccatatc ctcgataccc tccataaatg cggcgggttc tgccttgcct
     9721 tttgcaatct gcgtcagatt gttttcccat tctgcggtaa gctgcgggct tgtcaaggtg
     9781 tccggcagga cgcacacaag gttgataccg tcctttgtgg gaagtagctg cttgcctttt
     9841 cgctccacaa aaccgccctt taccagtttt tcaatgacgg cggcgcgggt ggcgggagtt
     9901 ccaagccctt tgcgttctgc gtccgggtcg gtgtcctcgc ttccggcgcg ctccatagca
     9961 gagagcagcg acgcttcgtt gtgaggtttt ggcggtgttg tgtcatgctc cgttaccgtt
    10021 gccgccgaat tttcaaaggt ctgtccctcg gtaaatggcg gcagggtctt ttctgtgtcg
    10081 ctgtctgcgt cgtccgtttc gggcttgttc tttaaggctg cccgatagcg gcggtcaagt
    10141 tctttccacc cgtcggacag tacccttttt ccccttgcgg taaaggactg tccggcacat
    10201 tcaaagattg ccgtaaccgc ttcaaaggta tgcggtgcgg cggtcgccat gagcagacgc
    10261 gccccggcaa gggtaaggat attcctttcg ctttccggca gcgcagcaag gtcggtcttt
    10321 gaaagctcca tagtcgggat aatggcatgg tggtctgata cctttttact gttcagcacc
    10381 tttgcaagct ccggcgtgaa gtcttttccc gccataaaag aaaatttccc acaaagcagc
    10441 ttgataatgc ccgccgctgt gtcgcccatg tcgtcggtta gaaatgcgct gtcggttctc
    10501 ggataagtaa gcaaacgctt ttcatacagg gcttgtgcaa ggtcaagggt ctgctttgcg
    10561 gtataaccga acaggcggtt tgcttccctc tgtaaagaag taaggtcaaa gagcttcggc
    10621 ggggctgcgg tctttttctc tttcacaagg gaagtacaga ctgtccgcga tgcttcgcaa
    10681 gtcgttttta atgcgtcggc ttctgcctta tctgaaattc tttcacttgc agcttccgcg
    10741 ccggataagg caaggcgcac atggtagtat ttttctttct tgaaagtggt aatcgctgcg
    10801 tcccggtcaa caagcatttt taaggtcggg gtctgtacgc gccccacgtt caaggtatga
    10861 ttgtaaagga cagagaaaag gcgggtggca ttgataccaa ttaaccagtc agcctttgcc
    10921 ctgcataatg cggaagcaaa gagcgcgtcg tattcctcgc cgttcttcag acgggaaaag
    10981 ccctctttga ttgcgctgtc ctccatagag gaaatccaaa ggcggcgcat aggtttcttg
    11041 cagcccgcca tttcataaac aaaacggaaa atgagttcac cctcgcgccc tgcgtagcag
    11101 gcattgacga cttcggaaac atctgcccgg tgtataagct cctttaaggt cttgaattgc
    11161 ttttccttgt cagccgcaac ggtgtacttc cattcctgcg gcaaaatcgg taagctgtca
    11221 tagctccact ttttatactg ttccccgtag gcggcagctt ccgcaagccc taccaaatgt
    11281 cccacgcacc aagaaacgat atagccgctt cctgtgagaa atccgtcttt cttttccttt
    11341 gccccaagta cggcagcgat tgtctgcgct acactgggct tttctgcgat aactaaaatc
    11401 aatgataaat cctcctttcg ggtaaaagaa aagagcaacc gatttttcga ttgctcctac
    11461 aagtgattgt ataaaattgt gcgatatgaa atagtaaaat aatttgacaa accggaagtt
    11521 gtttatttct tctttttcgg ttttggggct ggcagttcat catacattgc atgaaacaag
    11581 cctgtcaaaa attccttatt atcaacttca tctaccaaca acatctcttt tgctccctca
    11641 tagggtaatt catacggagc tgtcggcata taactaattg ccgattttac tggtttaaca
    11701 agtaatctat catcatagat accgcctaca atcttgccac gataataaat aataaattct
    11761 cctatcatag ctcgataagt aatttcttct aactcagata gctgccctaa aataaactct
    11821 aagtattcct tgcttgatgc catatttcca cctcaaattc ttatttgttt aactctgtca
    11881 tttcctatgg ttggaacaat tatatcatat tccatatcgc aagtacatct ttttatcgtt
    11941 ccgtctgtcg tttggtttcc agttttctga tacagtaccc cacacaaggg gactgtccct
    12001 tgtaaggaca ggaagtgcaa gccgggggat aaggaacagg cggcgcatta tcacgccgcc
    12061 cgttcggttt ctgtatcatc atcttttcaa agggattgtc ggtaaacagg gtcatagggt
    12121 ttcttcctcc gtttcttcat cttcgggagt ttccccggta tctgtttccg gctcgtcctc
    12181 gtcgtaatcc tcaaattcaa aatcttcaag gtcggtatcg ccctttacat tctgcttcgg
    12241 ctttagaaac ttaaagtagt agaacgctgc gccgccacca agcagggcaa agaggactaa
    12301 ggcaagcact acaccgggat tgccaccttt ttctttcggt tcttccggct gctctgttgg
    12361 tgttggggtg ggttctttgc ctgtgcagcc tgtcatactg gtaacacata ccgggcagga
    12421 cgtgtttacc tttccggctt cgcatttttc cgtacagtta cagctttccg gctttttggc
    12481 agcttcgccg ttttcggtca gtgccataag gtcggcttcg tccacttgat ttaggaaatg
    12541 tacggtctgt tccccctcgg cggcgcggtc aatgaggata tagaagtagt tgccgctttt
    12601 ggtggtaaca gtgataagct gcttattgcc gccgtaatcg tcaaccagtg ttgcgtttcc
    12661 ctttggggta aggggcggcg tttcttcttt ttcctccaca atcacgccgc tgtcattagt
    12721 tgcgtcctct gcgggcggct ctgtggctgc gccctgtgca taggcaggga cagaaaaacc
    12781 gcccataagg ataagggcgg cacaaagggc agtcagggtc tttttcagtt tattcttcat
    12841 ctgcattttc ctcctgttct tcgtagtctg cggcttcggt agcgggtgcg gcgttcatgc
    12901 cgggaatacc gccgccggac agcatagcgt taagttcctg cggggtcaga cgcatagctc
    12961 gtacaagctg tacgatttca aggttttccg cttcggtttt ctgcgcttcc agtcctttta
    13021 acttgttctg atactctgtg attttctcgc gggtctttgc gatttcctta ttgatacggt
    13081 cgattttatt gtttgccata ggtcatttct cctttctttt tggggctgct ttaccagttc
    13141 atcagcccgt agcctttgat acattgatag ttaaggtcat agctctttat cttgcaggcg
    13201 tcgccggaat tgccctcaac ggtatagacg cggctgccgt ccctgccaag cacaagcccc
    13261 acatggtc
//
LOCUS       DAEMIK010000123        13217 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEMIK010000123 DAEMIK010000000
VERSION     DAEMIK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.124"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..499
                     /gene="bcp"
                     /locus_tag="KPK87_002897"
     CDS             35..499
                     /gene="bcp"
                     /locus_tag="KPK87_002897"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG2539026.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            509..1252
                     /gene="yaaA"
                     /locus_tag="KPK87_002898"
     CDS             509..1252
                     /gene="yaaA"
                     /locus_tag="KPK87_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG2539027.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            1380..4037
                     /locus_tag="KPK87_002899"
     CDS             1380..4037
                     /locus_tag="KPK87_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG2539028.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4091..4193
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4303..5568
                     /locus_tag="KPK87_002900"
     CDS             4303..5568
                     /locus_tag="KPK87_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG2539029.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5598..6488
                     /gene="metA"
                     /locus_tag="KPK87_002901"
     CDS             5598..6488
                     /gene="metA"
                     /locus_tag="KPK87_002901"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG2539030.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6979..7422
                     /locus_tag="KPK87_002902"
     CDS             6979..7422
                     /locus_tag="KPK87_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2539031.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7453..9270
                     /gene="ftsH"
                     /locus_tag="KPK87_002903"
     CDS             7453..9270
                     /gene="ftsH"
                     /locus_tag="KPK87_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG2539032.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9448..12150
                     /locus_tag="KPK87_002904"
     CDS             9448..12150
                     /locus_tag="KPK87_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG2539033.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12184..12882
                     /locus_tag="KPK87_002905"
     CDS             12184..12882
                     /locus_tag="KPK87_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539034.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVNKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         5192 a         1514 c         2410 g         4101 t
ORIGIN      
        1 aaaattatta ataaaaacaa aaggagagat atttatgttg agtataggaa caaaagcacc
       61 agaatttaca ctagaagata aagatggaaa taaggtaagt atgtcagatt ttaaaggcaa
      121 aaaagtagta gtatattttt atcctaaaga taatacacca ggatgtacac gtcaagcttg
      181 tgcttttaga aatgcatatg atggatttaa aaaggaagat atacaagtta ttgggataag
      241 taaagatagt ataaaatcac atcaaaaatt tgcagaaaag catgaacttc cttttatttt
      301 actttctgac cctgacttag ttgctataaa agcttttgat gtatggaaag agaaaaaaat
      361 gtatggaaaa actgcattgg gagttgtacg tgcgacatat attatagatg aaaatggaat
      421 tattgaaaaa gtctttgaaa aagcaaaacc agacacaaat gcacaagaga ttcttgaata
      481 tttggaaaaa caggagtaat aagaatttat gattataatg ttatcaccag ctaaaaatat
      541 gaagaatata gaagtatttg atagagattt gagtttgcct tgctttatag ataacacaaa
      601 agaaatagtg gaaaatataa agacatttgg tatagaagat tttaaaaata aaatgaaaat
      661 taatgaaaaa ttagctgtat taaataaaaa tagatttgaa agcattaaat ttgatagatt
      721 aggaaaccct gcaatcttaa cttatgatgg aatacaatat aaaaatatag aagcagaaaa
      781 ttttacaagg aaagatgaag agtttgcaaa tagctgtatt cgaatcataa gtggattgta
      841 tggtgttgta aaaccatatg atagtatata tgaatataga ttagaaatgc agacaaaatt
      901 acaagtaggt gagtttaaga atttatacga atattgggga aatcgtatat ataaagaact
      961 aatcaaagag aaaacagcta tcgttaatct ttcatcaaat gaatacagca aatccataga
     1021 aaaatttatt aaagatagtg atacatatat tacatgtact tttaaagtaa ataaaaatgg
     1081 catattgaaa gtggaatcta cacaagcaaa gaaagctaga ggtatgatga caaaatacat
     1141 tgtaaaaaat aggataagag atattgaaga actgaaaaag tttgatttag aaggttataa
     1201 gtataaagaa aacttatcta acaatagtga atacatattt gtaaaagagt agaagttttg
     1261 ttaaatatta aatggcattg ttaaatttag gtaaaatata cttagtgtgt attgaattga
     1321 acttgatttt aaaatataat tattcttaaa ttaaaaaaag aaaggagtgg tcttccaaaa
     1381 tggaaaatca caaacagatg ccttggcatt caagagcaac gcatgaaatt ttagaaagtc
     1441 tagattcatc agaggagggg ctcaccgata ccgaggcgga aaaacggctt aaagagaatg
     1501 gcttaaatga acttagaaaa aaaccaccta gaacaacttt acaaatgctt tggagtcaaa
     1561 ttacagatgc tatggttatg atactaatag gagcagcaat attatctttg atatttggcg
     1621 aatttacaga agcttttgta atactaataa ttgtaacagt aaatgcagtt attggaattg
     1681 ttcaagagaa aaaagcagaa tcatcattag aagcattaaa aaatatgaat tcacctacag
     1741 ctcgtgttat gcgtgatggt gaagaaaatg ttattccagc aagtaatctt gtagtaggag
     1801 atattgtttt tttagaagat ggagctatgg ttccagcaga ccttcgttta atagaaacat
     1861 caagtttaaa aattcaagag gcttctttaa caggagaatc tgttccatca gaaaaagatg
     1921 cagatacaat cttacctaaa gaatgtgtat taggagaccg tagcaatatg gcgtatacat
     1981 cttcaatagt cacatatggt agaggtgtag gtgttgtagt agcaactggt atggatacag
     2041 aagtgggtaa catagcacat cttttagatt cacaagatga ttttgatact cctataaaac
     2101 gtaaacttaa tactgttgga aagacattaa gtgtcatagg tattattgta tgcgttgtta
     2161 tatttgcaat aggtgcattt tatcagagac ctctcttgcc aatgtttatg attgcaattt
     2221 cccttgccat atcaattatt ccagagggat tacctgcaac agctactatt gtaatggctt
     2281 taggcgtaca gcgtatggct aaaagaaatg ctttaattag aaaattacct gctgttgaaa
     2341 cattaggagg agctacagta atatgttgtg ataaaactgg tacgttaacc ttaaataaaa
     2401 tgactgttac acatattgct gtcaatggag attttgaatc tggaaaagtg acatcaatag
     2461 atactgcatc tgaaaaacat ccattagtgt ataaagagat tgtttatgct ggtgcacttt
     2521 gtaatgatgc atgtattaat ccagataaaa cagatgaaat attaggtgac ccaacagagg
     2581 gcgcattaat ttttatggcc aaaaaatttg gaatagatca agaagctctt gaagaagaat
     2641 atccacgtgt atttgaacag ccttttgatt cagatagaaa atgtatgaca actgttcata
     2701 atatagatga aaagattatt gcttatacta aaggagcagt agacgaaatt cttgacctat
     2761 gtactaaaat attaacttca aaaggtgaaa gaaacattac tgaagtggat aaaaagaata
     2821 tacacgaatt atgtcttaac atgtctaaag atgcattgcg tgtacttggt tttgctaagc
     2881 gtgagatttc atctatacca aaagaagata gtgaaaatat agaatatgat ttaacattta
     2941 taggtatggt tggtatgatt gacccaccaa gaactgaggt aattgatgca gtaagtactt
     3001 gtaaagaagc aggtataaga accataatga taacaggtga ccataaagtt actgccacaa
     3061 caatcgctca tgagttagga atatggagtg aagaaaatac tgtgatatct ggtgatgaac
     3121 ttgataattt gagtgatgat gaactagatg aagctgttaa aaatactaca gtatttgctc
     3181 gtgtatcccc atttgataaa cttagaatta tacaatctct aaaaagaatt ggagaagtac
     3241 cagcaatgac tggggatggt gtaaatgact ctcctgcctt aaagtctgct gatattggta
     3301 tagccatggg tataagtggt acagatgttg caaaagattc ttctgacatg attttaatgg
     3361 acgatagttt tacaacaatt gcttatgcaa taaaagaagg tagacgtgta tatagaaaca
     3421 tacaaaaagt tatacaattt ttgcttgttg gtaatatagc agagatactt acgttgtttg
     3481 tagcaatatt attaaattgg gatactccgc ttcttgctgt tcatatttta tgggtaaacc
     3541 ttgcaactgc tacgcttcca gcccttgctc ttggagttga cccaccaagc agaaatataa
     3601 tgaagcacca gccagtgaaa tcaaccactt tgtttgaaaa aaatcttata caaagagttg
     3661 ttacccaagg tatatttgtg gcaataatga ctattttagc atataaaatt ggtcttaata
     3721 tagaaaatca ttctgtaggt caaacaatgg cattttgtgt attagcattt tcacagatgt
     3781 tacgagcatt tagtcagcgt tcaaatacag attcaatgtt taatagaaat aatggtaaaa
     3841 atccatttct tttaatctca tttttaactt ctgcattttt aatagcagta atactgtttg
     3901 tacctgtttt tagaaatgct tttaacttaa cagttttaaa taacttagag tggcttataa
     3961 cgattgtttt agccataatg tctgtaattc aagtagaggt tgggaaaatt attaaaagaa
     4021 aaaaattaaa aaaatagttg acaaaattag gaaaaatata taagatataa aacatagaaa
     4081 aattaaataa atcttatcca gagaggtgga gggactggcc ctatgaaacc cggcaaccag
     4141 tatatttata tacattggtg ctaaatcctg cggcgaaagc tgagagatga gtataattaa
     4201 gcttatacag agcctgagtt atatactcag gctttattta ttttaactct catctggtaa
     4261 atagtttaat tttttttact aaaatctaag ggggatacaa gaatgtataa taaagaaaca
     4321 atatgtgtgc aaggaaatta taaaccaggt aatggagaac caagagtact acctttatat
     4381 caaagtacaa cttttaaata tagcagtata gaccaacttg ctgaattatt tgatttaaaa
     4441 gttgatggac atatatattc aagaataagc aatcctacta ttcaagcttt tgaagaaaaa
     4501 ataagtttac tagagggtgg agtatcttct gtagctgtat catcagggca atctgcaaat
     4561 atgttggcag ttttaaatat atgtaaatca ggagatagta tactttgttc ttcaaaagta
     4621 tatggaggaa cattcaattt actaggacct agtcttaaaa aatttggtat agatttaata
     4681 tcgtttgact tagattcaag tgaagatgag atagtagaac ttgcaaagga aaatactaag
     4741 gttgtgtttg cagaaacact tgcaaatcca actcttgaag tcatagattt tgaaaaaata
     4801 gcaaatgtag ctaagagaat taatgttcca tttattgttg ataattcatt agcatctcca
     4861 gtgctttgta accctttaaa gtatggagca aatatagtta ctcattctac cacaaaatat
     4921 ttagatgggc atgcttcaag tgttggagga attatagtgg atggtggaaa ctttaactgg
     4981 gataatggaa aatttccaga attagttgag ccagacccaa catatcatgg tataagctat
     5041 actcaaaaat ttggaaatgc cgcatatgca actaaagcaa gagttcagtt gcttagagac
     5101 tatggaaatt gtttaagccc attcaatgcg tatcttacta atttaaatgt tgaaacacta
     5161 catcttagaa tggagagaca tagtgaaaat gcacttaaaa tagctagatt tttagaaaaa
     5221 catgaaaatg tagattggat taattaccca ggacttgaag ataacaagta ttatgagaat
     5281 gccaaaaagt atttatcaag aggatgtagt ggtgttttat catttggagt aagaggtggg
     5341 ttagaaaatg ccaaaaaatt tgtggaaaaa ttacagatag catctttggt tacacatgtt
     5401 tcagatgtaa gaacttgtgt tatacatcca gcttcaacta ctcatagaca attaacagaa
     5461 gaacaattaa ttgcatctgg agtattgcct tcactaataa gattatctgt tggaatagaa
     5521 aatgtagagg atttaatagc tgatttaaat caagctttaa atttctaata ttctaagaga
     5581 aagagaagtg aaaagatatg gctttaatac taccaaaggg attacctgtt attaataaat
     5641 tattagatga aggaatagat gtaatttata aagaagattt taaaaaggaa ttaaagtatg
     5701 aagaaaacat agacactaag atagctatac taaatctaat gcctataaag atagatacag
     5761 aattagattt actaagaagg atagataaaa caggattcaa cgtgtctgtg gaatttatta
     5821 aaatttcaac aagagaaagt aaaagaagtt gtaatgaata tgttaaagaa ttttataaaa
     5881 catttgatga agccaaagga gagtattttg atggctttat aataacagga gctccagttg
     5941 aacaaatgga gtttgaagaa gttgattatt ggaatgaact agaagaaatt atggattatt
     6001 ctaagagaaa aactaaatct acattatata tatgttgggc agctcaagca agtctttaca
     6061 aatactacaa tgtaaaaaaa cttccactaa gtcaaaaatg ttttggagta tttaagcata
     6121 aggtagataa agatagtaaa atagtagatg gatttgaaaa tgaatttttt gctccacatt
     6181 caagacatac tactgtaaat attgaagctt tgaaagagaa taaagagttg agcattgtga
     6241 gtcattctaa agaagctggt ccctatataa ttacaaattc aagagatgtg ttcgtaatgg
     6301 gtcatagcga gtatgacaaa tacactcttg ataaagaata taagagggat ataaataaag
     6361 gagataaaat atctatacct caaaattact atattaatga tgacccttca gaagagccaa
     6421 ctgtaaaatg gaaaaaacat tcagaattgt tgtttagaaa ttggataaaa aactatttga
     6481 tacaataaat tttaagagta tataaatgtt aactaaaaaa atatagaaat ttataggtat
     6541 aaagttaatc ttaatgatac tttatacctt atttttttaa aatttgacct tataatagta
     6601 gttttatatt ttaagaaaat cactttaggt gtatgtagat agaggtggct ttaattttat
     6661 atatggtgcg ataatgggag actaaacctt tatagtatca ataaatttgt attttaactt
     6721 ttaaaagaga ttaaaaaggg agaaatcagt gtaaaatata tatatagatt ttttattaaa
     6781 ttttattaaa aatatcctgt ataaaattaa atatcaataa gataacaaaa tttcaattga
     6841 agttacaata ttgtaattga caataaatac ttaacactat aatatttaat acattaaaca
     6901 ttatagtgtt aagtatttat ttacagtatg cacttcagcc taaatttgaa ataaagtatt
     6961 ttcgtaggga gcgtttgtat ggatgagatg agttttaaga aagagctact agagttgact
     7021 agagatataa atatgaaatt tacaacttta ttaagtgatt tttatcagcc acttggtatt
     7081 acagcagtgc aagctttgat tttatctgag ttatgtgaac atggagagaa aaaaataagc
     7141 gatttaggga agaacttaaa catgacaaat agtaatgtat cagtaatctg tcaaaggctt
     7201 gaaaaaaatg gctttttaaa tcgcataaga gatatagaag accaacgaat tgtaaaagta
     7261 aaagttacaa ataaatcatt ggatatacaa gaacatattt caagtagtat ttttgatagt
     7321 tattttgaaa atatgaccag tgaaaaattg caagatatgg aagatataat agaaggttta
     7381 gagaaattaa ataaactttt aacttacatt gattgcaaat aacataaaaa taatttaaag
     7441 gagaataaaa ttatggatga acataaaccc caaaaaaggt cagcactcta ttattataca
     7501 gttataataa tagtagtaat gtttttaaat gctttaatat ttccaagttt aatggataaa
     7561 aaaatggttg agattgacta tggaaagttt ctcacaatgt tggatacagg aaaggttaaa
     7621 tctgttgaga tagaaaataa tagaatagca ataaatccat ctagtagcaa ggataagaat
     7681 atatacataa ctggtagaat ggatgaccca gaattggtag atagactaaa aaatgcaaaa
     7741 gtagaattta ctaaggttat tcctaaggaa aattcacctc ttttaagtat tttacttaca
     7801 tggataatac ctataggtat tcttatgcta tttggtaact taatgatgaa gtctatgcaa
     7861 aagcgaatgg gtggtaatgc tatgcaattt ggaaaaagca acgccaaaat gtatgtatct
     7921 gctcaaagtg gaaaaagatt taatgatgta gctggtcaag atgaggcaaa agaagcttta
     7981 actgaaatag tagatttctt acacaatcca gaaaagtata aaaagatagg agcacaaatg
     8041 cctaaaggag cacttttagt aggacctcca ggtacaggaa aaacactcct tgcaaaagct
     8101 gtagcaggtg aagcaaatgt accatttttc tcaatatctg gttctgagtt tgttgagatg
     8161 tttgtaggaa tgggagcttc taggattcgt gatttattta aacaagcaaa agaaaaagct
     8221 ccatgtatag tgtttataga tgagattgat actattggta aaaagcgtga taatggaagt
     8281 ggattaggtg gaaatgatga acgtgaacaa actttaaatc agttgttaag tgagatggat
     8341 ggttttgatg gtggaattgg agtagtaata cttgctgcta caaatagacc tgacagtctt
     8401 gacaaagcat tacttcgtcc aggaagattt gatagacgta taccagttga acttccagac
     8461 cttggaggac gtgaagctat attaaaggta catgctcaaa atgtaaatgt aagctcagat
     8521 atagattata atgcaattgc aaggtctaca tcaggagcat caggtgcaga acttgcaaat
     8581 attgtaaacg aagcagcact tttagcagtt aagtctggca gagatatagt agaacaacag
     8641 gattttgatg aatctgttga aactgtaatt gctgggtatc aacgtaaagg agctgtaatt
     8701 tcagacaagg aaaagaaaat tatagcatat catgaaatag gtcatgcact tgttgctgct
     8761 atgcaaaaac attctgcacc tgtccataag attactataa tacctagaac ttcaggagca
     8821 ttgggatata ctatgcaagt tgctgaagat gagagtgtac ttatgtctaa agaagaagca
     8881 attgataaaa taactacttt tacaggtggt cgtgctgctg aagaggttat ctttaacact
     8941 tgtacatcag gagcatcaaa tgatatagaa caagctacta aaattgcaag agctatggtt
     9001 actcgccttg gtatgagtga agagtttgat atggtcgctc ttgaaactgt aagcaatcaa
     9061 tatttaggag gagatacttc tcttgcttgt tcttccgaca ctgctgctaa aatagatgaa
     9121 gaggtattta aaatagtaaa ggcttgtcac aaaaatgcaa ttgaaatctt agaaaataat
     9181 attgataaat tgcatgaact tgctaattat ttattagaaa aagaaactat tacaggtgat
     9241 gagtttatgg aaatacttaa cttaaactaa aatgattata cacaagataa aaatgcctat
     9301 tgttaggcat ttttatttta caactaaatt ttttaaattc atatttaaat ctttacataa
     9361 tcttaatcat taaaaataca tcttaactac aattttatat cggaattgat attataatag
     9421 taatgaatgg tgtaaggagg ttttttaatg gatagaccta atcctgatat tctattagaa
     9481 aaaattaaaa atgaagaaga aaaactaagt agaggtcaat taaaaatatt ctttggttat
     9541 gcagctggag taggaaaaac ctactctatg ttggaatctg cacaaaattt aaaaaaagtg
     9601 ggtgtggatg tagtagttgg atatattgaa ccacacacta gaccagaaac attggcttta
     9661 cttgatggct tggaaattct tcctgtaaag gagatagaat ataaatctat aaaactaaaa
     9721 gaatttgatt tagatgctgc tttagcaaga aaaccagaag ttatattagt agatgaattt
     9781 gcccattcta atgttgctgg gttaaggcat acaaaaagat ggcaagatat agaagaattg
     9841 ctgttagcag gcataaatgt ctacactact gtaaatgtac aacatttaga aagtctaaat
     9901 gacattgttg agataattac aaacgtatca gttaaagaga ctataccaga taagtttttg
     9961 gatacaaata cacagttaga acttatagat gttgaacctg atgtacttct tgaaaggttc
    10021 aatgaaggta agatatataa gaaagaacaa gctactagag caaaaaataa tttttttata
    10081 aaagataact tggttgcact tagagaaatc gcacttagaa aaacagcaga gagagttaat
    10141 aaagaagttc aaatgaccag actatcaaag ggtgatgtaa ctgtaatacc tacatcagat
    10201 acacttcttg catgtataag tccatctcca tcttcagcaa aagttattag aactgcatcc
    10261 agaatatcag acagtacatt tgcaaaatgg atagctttat atgttgaaac tccaaacaca
    10321 gcaagacttc catttgatga acaaaaacaa ctacaaaata acctaaaatt agctaagaaa
    10381 ttaggtggag aaataattgt tttacatggt gaaaatataa tagagcaaat acttagaatt
    10441 gcaaaactga gaaatgtaac aaaaattgta ataggtagaa atcattcaag taataaaaga
    10501 tttagtaaaa gatttaaaaa agatatagtt gacaaattaa ttgatgaagt tgattatata
    10561 gatatacata taattccata taaatttgcc tcagatgtta aatacaaacc taaaaaggat
    10621 aaatcatcaa taaaatctaa atttaaaatt agcaaagttg attttataaa acttatattt
    10681 attactttga tgataagtat tttagcatat gcagtacaaa gcatgggatt tataagagaa
    10741 aatatattgt taatatatat gttgggtgta gttttagttt ctatgtggac taaagggtat
    10801 agtacaggta taataagttc tgtatttaat atagttcttt taaattattt ttttacagca
    10861 ccattatata cattatctat agcagattca aactatatag taacactggt tgtattctcg
    10921 atagtaggta taataactag cactcttacg tctaagatac agcatgaagc agaaactgct
    10981 gctaaaagag aagaaaatac aaaaatgata tatcaaataa ttaagggatt tttaagatta
    11041 tcaaataaag atgatatagt gaacaaaggt atagaacttt tgtctcttag cttaagtaga
    11101 gatgtaattt gttatttaaa tgtagataag cataataaat caaagcttta caaaaaaaat
    11161 actaataata aggataaaaa tgatttggat agtgaagatg aaaaagctgt tgcaagttgg
    11221 gttttatcca attcaacagt ggcaggaaat gacacggata cacttcctgg ttcaaaggga
    11281 tactatatac caattgtagg aatgaattta actttaggag tagtgggaat atcatgtatt
    11341 gattcgaaat tggatacaga agatataagc ttaattgaaa caataattgc tcaaatggca
    11401 atagcgcttg atagagaaat cttgtcagaa gctaaagaaa ataccaatct tgagattgaa
    11461 agggaaagat taagaagtaa tcttttaaga gcagtttcgc atgaccttag aactccacta
    11521 gcaggaatat ctggtgcagt aagtacaatt ataaaaaaca agggtactat aggtcaagat
    11581 ataatagatg aattacttag tggtgtatat gaagatactc agtggttaat aagacttgtt
    11641 gaaaatttac ttagtatgac caagatagat gaagggaaat tggaagttaa aaaacattct
    11701 gaactggttg aggaaattat atcagaagcg ttacaaaaaa taaaaaaaag aattgaaaat
    11761 gcacttatag atattaatat accagaacag atattatttg ttcctatgga tgcaaaatta
    11821 atagaacaag ttttaattaa tttaatagat aattctttga aatattcaaa ggaagattgt
    11881 aagatagaga taaatgtata tgagaaagat gattatgttt ggtttgaagt atctgataat
    11941 ggtcctggta tatcaaagga acttaaaaag catatctttg acagattttt tactggagaa
    12001 gaaggagcta aagattccag aaagggtgtt ggacttggat tatcaatttg taaatctata
    12061 attcaagctc ataaaggtga aattatggta gaaaacaata aagataaggg ttcaactttt
    12121 aagtttagtt tgcctaagga aaatgaatag ttttaaattt taaaattagt tttggaggaa
    12181 gaaatgagta aaaaacttat attgttagta gaggatgata agacaataag aaaatttata
    12241 tctactgcac ttttaacaca ggattatgat gtcaaggagg ctataactgg aaaagaaggt
    12301 atctctattg ctgtctcata ttctccagat gttgttcttt tagatttagg cttagaggat
    12361 atggatggaa tagaggtaat aaaagctatc agacaatttt caaatatacc aataattgta
    12421 gtttcagcta gagagcaaga tagagacaag gtagaagtat ttgatgcagg ggcagatgat
    12481 tacttaacaa aaccattctc catagtagag ttattagcaa gagtaagagt agcatttaga
    12541 cattcacaag tagaagctca acaaaaagat gatgttaaat ctacgtttga agtaaataaa
    12601 ttattgattg attttgataa aagaaaagtt attgttgatg atgttgaagt ccatttgaca
    12661 cctattgaat ataatattct ttcattactt gcaaagcatc atggaaaagt gcttactcat
    12721 aattttataa taaaagaaat atggggtagt gttataggga atgagactaa gtctttaagg
    12781 gtctttatgg caacgttaag acgaaaaata gaaaaacaac ctgcaaaccc aagatatata
    12841 attacagaag ttggtgtagg atacagatta aatgatgaat agttgtaata tattaattat
    12901 tagattatgt gcttattaat atatattaaa attatatagt ttagttaaaa taagaatgaa
    12961 gtgtatgtgc ttgttgtcag tttagcacat gcattttatt ttttagcttt tttagtatca
    13021 ataatttttt agtatcaata atatgaaata tgttttaaaa taagtatata gtgtaatctg
    13081 taataaaaac tttggttaaa taataaatgt atttaacact taccttataa taaatttaat
    13141 ataaatatta aataataatt attaaaatag tgtaaaataa ttacagtaat gattataaaa
    13201 aatttttaga atgtaaa
//
LOCUS       DAEMIK010000124        13194 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEMIK010000124 DAEMIK010000000
VERSION     DAEMIK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.125"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            69..770
                     /locus_tag="KPK87_002906"
     CDS             69..770
                     /locus_tag="KPK87_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG2539035.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDKEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSIKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1041..1859
                     /gene="larE"
                     /locus_tag="KPK87_002907"
     CDS             1041..1859
                     /gene="larE"
                     /locus_tag="KPK87_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG2539036.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1914..3062
                     /locus_tag="KPK87_002908"
     CDS             1914..3062
                     /locus_tag="KPK87_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG2539037.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3198..4586
                     /locus_tag="KPK87_002909"
     CDS             3198..4586
                     /locus_tag="KPK87_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2539038.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4615..5313
                     /locus_tag="KPK87_002910"
     CDS             4615..5313
                     /locus_tag="KPK87_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539039.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5316..6749
                     /locus_tag="KPK87_002911"
     CDS             5316..6749
                     /locus_tag="KPK87_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2539040.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6771..7508
                     /gene="truA"
                     /locus_tag="KPK87_002912"
     CDS             6771..7508
                     /gene="truA"
                     /locus_tag="KPK87_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG2539041.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7619..7826
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8079..9278
                     /locus_tag="KPK87_002913"
     CDS             8079..9278
                     /locus_tag="KPK87_002913"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG2539042.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9525..10571
                     /locus_tag="KPK87_002914"
     CDS             9525..10571
                     /locus_tag="KPK87_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG2539043.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10679..11842
                     /locus_tag="KPK87_002915"
     CDS             10679..11842
                     /locus_tag="KPK87_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2539044.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKIYLRDRKPKINTLYEKLVEDLSRDCSSREVQSVVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASRFIGEAFKIYFDNNNC"
     gene            complement(11931..12311)
                     /locus_tag="KPK87_002916"
     CDS             complement(11931..12311)
                     /locus_tag="KPK87_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG2539045.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12314..12973)
                     /locus_tag="KPK87_002917"
     CDS             complement(12314..12973)
                     /locus_tag="KPK87_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG2539046.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5303 a         1313 c         2271 g         4307 t
ORIGIN      
        1 gtattaaaaa tctgacaaca aaaataaaac tttcaaaaaa ttaaatctaa aaagaatagg
       61 agataaatat gagtattgaa gatttatatg attttgaatg tgttcctttg aacaatgaga
      121 ttgatattga tgttttattt gaaaagatga tagaagatgg aaaagaaaaa gaatatacac
      181 caattatagt cattgatgaa aaagtagggc tattaagaaa aaatatagat atgattacta
      241 aagagtatgg ttctcttgaa aactataggg agtattgttt aacaaaatat aatgaaattg
      301 atgtaaatca attttttgat tttaggaaga gtgagatttc agaattatta aaggagctaa
      361 ttgatttaga agatgatttt tatgattata aacattttag acccattatg aatatagata
      421 tatttcatga atataacaaa gtttacatag ctaaaattcc aacaattaaa ccatatgaag
      481 tatttgcata catacctatt ggcggattta atgattgtcc tagagataaa gaacatatag
      541 ctattgcaaa atattggtat gaaaagcatg gaatctttcc aatcgcaatt ggatgtgata
      601 cagttcaata ttctataaaa aatttaacta aagatggtaa aaaatttgat gacttatgta
      661 ttgaattagt attttattgt gaggatatta ttatacaagg gtatgaaacc ttaaaggctc
      721 ttaaggatgt attaaaacgc tctacaatag tgcttttttg gtgggattga tattctatca
      781 cttagacagc attaaattgt tagtcaccaa gaatccactt gctttagtta tggggagtgt
      841 gtcaaactag gtttttatac acagtgtagc atagtaatat gtttaaatta caaaggttaa
      901 aaaaatactt taatattttt aatattataa ttaatagatt taaaggtaat tataatttat
      961 aaaataaata ctttatatgt tataatcatt aagaatgtaa aaaaataata tatagtttta
     1021 tagttcatat ttttccagat atggaggtaa attttgtgaa ggaaaaagaa aaattagata
     1081 aattaaaaaa aatgttactt gaattaggca gtgttgtagt tgcttattct ggtggagtag
     1141 atagtaattt tttgcttaag gtcgcaaaag atacattagg tgaaaatgtt atagctgtga
     1201 ctattcatgc aatgatgcat tcaagtaggg aaattgaaga ggctaaacaa tatactcaaa
     1261 attttggtgt taaacatatc atattaaata tagaaaactt tgatttaaaa gaatttaaag
     1321 aaaatggtgt tgatagatgt tatcattgta aaaaatatat attctctaaa ataaaagagg
     1381 tagcaaaaga acataatata aagtacatag tagatggaac aaatatagat gatttaggag
     1441 actatagacc tgggcttaag gcattgagtg aattaggagt aattagtcca ttaaaagata
     1501 gtagtttaaa aaaagaagaa attaggtcgc tttcaaaaac attaggatta aatactttta
     1561 ataaaccatc atttgcatgt cttgcgagta ggatacctta tggagttgaa ataacagatg
     1621 aaaatttaag aatagtagaa aagagtgaag aatatctatc caatttaggt ttttctcaat
     1681 ttagagtgag aatgcatgga gatatagcta gaatagaagt tggacaggaa gaactaggta
     1741 aattttttga aaataacaat tttaataaag ttgatactaa attaaaaata tttggcttta
     1801 aatatgttac actagatatg tcaggatata aaatgggaag tatgaattta aatgtgtaga
     1861 ttattttaaa aataaataag ttttatagtt aatataagga aacaggaggt tttatgaaaa
     1921 attatacgtt aatatcgcca tgtttttttg gaatggaaaa aatgctggct agagagatta
     1981 ctaatttagg atatgaaata ataaaaacag aagatggaag aataacatat aaaactgatg
     2041 agtttggaat agcaaagtca aacatgtggt tgagatgtgc tgaaagagta catcttaaaa
     2101 ttgctgaatt tgaggcaaaa agctttgatg aattatttga aaatacaaaa agaataaact
     2161 ggtctagata tataccttat ggagctcaat ttcctatatc aaaagcttcc tctataaaat
     2221 caaaattata cagtacacca gatgtacaag ccatagtaaa aaaggctata gtagaaagct
     2281 taaagaaaag ttatttggag gatggtttgc ttaaagaaga taaggagaaa taccctattt
     2341 ttgtatttat acacaaagat aaagtaacca tttctataga tacaacagga gatgctttgc
     2401 ataagagagg atatagagaa aaggcaaata aagctccaat aagagagact ttagctgctg
     2461 gtcttattta tttaactccc tggaaagcag gtagagtttt agtagaccct atgtgtggtt
     2521 ctggtactat attgattgaa gctgctatga ttggtataaa tatggcacct ggcttaaata
     2581 gagagtttat atcagaaaag tggagaactc ttgataagaa aatatggtgg gatgtaagaa
     2641 aagatgcttt taataagata gataatgaat ctaaatttaa gatttatgga tatgatatag
     2701 atgaagaatc tatagatata gctagagaaa atgctgaaat agcaggtgta gatgaatata
     2761 tagagtttaa tgtaggagat gcaactcaat ttaaaagtga agatgagttt ggatttataa
     2821 tcactaatcc tccttacgga gaaagattag aagataaaga cagtgttaaa cagttgtata
     2881 aggagcttgg ttatgcattt agaaaattaa aaaattggtc gtattatttg ataacttctt
     2941 atgaagactt tgaatatgag tttgggcaaa aagcagataa aaaaagaaaa ttgtataatg
     3001 gtatgttaaa aactaacttt ttccaatatc cgggtcctaa gcctcctaga aataataaat
     3061 aagttttaca tgatttattt gtaaaatata aaattttatt aaaataagta aaacattatg
     3121 atgccttgaa acgtctaatt attgtgagga gatttttgtg aacaaattta aagatttata
     3181 ggattaggag gagttaaatg aaagtgttaa agcttattac tgtagtagcc ctgatgatgt
     3241 tatttgttac tggatgtaat ataaaaactg aatctccaga acaattagcg aagatacctg
     3301 actatgatga tacaaaaaaa gtattgtatg atggtattga ccagttacta aaaccagatt
     3361 catctatgat attgccttct aatacaaaag aagtaggaaa aataaataag gtagatttaa
     3421 acagcgatgg aattgatgaa ttagtagtat ttgaacaaaa agaagattta agtaataatg
     3481 tgagtcaggt agggtttgta accttgagtt acaatggaga aaaatacgta cttggagacc
     3541 attttttaga aaatggcgaa tcaatagaat atgctaattt ttatgatttg gactcagatg
     3601 gatataagga agttatctta ttggttaaat ccaaagacaa aacgaatatg catatatata
     3661 aagtagtaga taatgaaata acaaaaatat atgacttgga tgcatcatgg cttcaaaata
     3721 aagaagattt taatgacatg aaagtaaaaa ttggatatat agatggagat gacaagttag
     3781 acatattgat gcttcatttg aataataaga ctaatgaaat gtttgcaagt gttgcaaatt
     3841 ttgatgggaa aatgaaaatc aaagattctg ttaagtttga aaatgtaaaa aacttaagtg
     3901 aattatatat aacattaggt aatgttgctt catctgttgg aaattcttct gcagctataa
     3961 aaggtattgt acttgatatt cctatattga aagataataa ctatattacg cagatacttt
     4021 atatgaaaga tgggaaaatt aacaaagctt ttagtgatta tgataaaact attactaaat
     4081 cttattatat acctgtagat gatattgaca aagataagat tatagaaata cctatagttg
     4141 caggcagtac aggtaataat aaaaatactt atagttctaa atcatctgca actataagtt
     4201 ggtatagatg gaatggaaaa gaaggtgctg actcaagttt aatatttaca agtcagatat
     4261 attataatta taaatataat tttaaattat ttattccaaa taatttattt gataaaatac
     4321 ttgttgaaca ggaatttgtt ggagaaaaag cagtatttaa attttactat tttgactatt
     4381 cagaccgtaa gaatttattt aatatagtag tagagagtaa aaataaactt gaagatagaa
     4441 aaaatacagg tactcaaaat tctattgtac ttcaagagag cgatgaatat actttcttac
     4501 tagttgtaaa caatgcaaat gaaatggata agctagatat gactatagac gcattaaaag
     4561 aatatttctc attaatatat ggttaatatt atactcaatt ggaggtttta ttttatgaag
     4621 gaaaagatac ttatactaga agatgaaatt ggtattagaa gttttgttag tataaactta
     4681 aaaagggaag gctatgaaat agttgaggca ggtactggaa gagaagctat agaaaaaatg
     4741 acaacagaaa aggatattac aatagctctt ttagatgtaa tgcttccaga tataagtggt
     4801 atagaagtat gtaaatttat aagggagaat tttgaccaag taggtataat aatgcttact
     4861 gctaaagctc aagaagatga taaaatagaa ggatttatct caggagcaga tgattacatc
     4921 ataaaaccat ttagtataaa agagctttta gtcagagttt ctgctcttct tagaagagta
     4981 gcaaaggatg attcaagtgt taagtcgagt gagatagttt ctccaccatt tatacttgat
     5041 atagacaaaa gaaaattatt taaaaatgga aaggaaattg agcttacacc aactgaattt
     5101 tcaatagtga aatatctaat atcaaatgct aagcagtcgt taagtagaga ccagatatta
     5161 gacgaagtat ggggaactaa ctatttatat gattttaaaa tagtagatgt aaatataaga
     5221 aggataagaa ataaaataga agatgaccca tcaaaaccaa aatatataca aactatttgg
     5281 ggttatggat actgttttag aaaggaagaa taatagtgtt acattttgaa ggtctgagaa
     5341 aaaaagtaat aaaaaactat tttataataa ttattataat ggttactctg tttgaaggat
     5401 tgttcatgtt ttacatacaa aactactatt atgattctgt caaacagcta cttgaatctg
     5461 aaataaaata tgctgatgaa tataatgcta ttacaatgga aactacaagt tttgagaaaa
     5521 aagtaaaaaa tatatttgat aaacaaccct taactaaaaa ttcagaattt ggaatttcaa
     5581 taatagataa agataaaaat attatattag accaatatgg ttttaaaagt aaagaaaaag
     5641 caaactatga agatgttaac aatgcattaa aagatataaa aactaaaaat ttaacacctt
     5701 atacttatag aataccagat acaggggagc atgttatgag tatctcctta ccacttaagg
     5761 taaataatat aatagaaggt gttgttagat atacagtatc attagatgct attgataatg
     5821 caatattaaa gcaagctaca tggcttatat tagcaggaat atttatactt attatagcta
     5881 tactaataag tcttaaattt gctgagactc ttataaaacc attacgagag ctaaagaaat
     5941 ttgcaaatga attggctgta ggaaattata atataaaatt agaaaagatg aagattgtag
     6001 atgatgaaat tggtgattta gcacagacat ttgaacacat ggcacatgag attgacaaaa
     6061 gtgaaaaatt aaaagaggaa tttatatcat ctgtatctca tgaactaagg actcctctta
     6121 catctataaa ggggtggagt gaaaccttag gttatgaaag tataacaaga gaagaactag
     6181 acttaggttt aggcattata caagatgaaa cagaaagact tataaaacta gttgaagaat
     6241 tattagactt ttcaaggctt tcttcagaca ggataaaact tcatgttgat atagttgatg
     6301 ttgaaggttt aattgtaggt gttgtaaatc aacttaaagt taaagcagct gaaaaagata
     6361 tatcactttt atttgaattt gaaaatgaat ttatagagaa cattcaagga gataaaaata
     6421 gattaagaca ggttctcata aatttaatac aaaattcatt taaatttacg agtcaaggtg
     6481 gttacataaa agttgtagct tctcaagatg aagagataac aaccatatca gtagaggata
     6541 atggttcagg tatagaaaag caaaatttaa ataaagtgtt agataaattt ttccaagaag
     6601 attataataa ggctggtagt ggattaggac tagcaattag taatgaaatt gtaaaacttc
     6661 atggtggaag aatgaaaata gaaagtgaaa agaatgttgg aacaaaaata acttttaata
     6721 taaaaaataa atttgctaaa caagcataaa ttcttagaaa gaggattaag atgagaaaca
     6781 taaaaatgat agtagcatac gatggttcaa gatataaagg atatcaaaaa ttaggtgaca
     6841 ataatatgac tattcaagaa aaacttgaaa atgttttaag taaaatgact aatgaaacag
     6901 tggagataat aggttctgga agaactgata tgggagctca tgcaagagga caagtggtaa
     6961 attttagaac taattgtatg gattctttgg ataaaattca aaaatattta tatgaatatt
     7021 taccagaaga tatagttgta aaaactgttg aagaagttga tgaaagattt cattcaagat
     7081 acaatgtaaa atcaaagact tatatgtata aaattgacaa taataagtat catagtcctt
     7141 ttataagaaa atatgctact catgtaagta aaaaattaga tttagataga atgagaaaag
     7201 caagtgagta tttagtagga gaacatgatt ttaccagttt tgcttcttct aaatctaaga
     7261 aaaaatcaaa tgtaagagaa atttattcta taaatataaa agaagatgat aatgttatag
     7321 aaatatatgt agaaggaaat ggatttttgt ataacatggt aaggataata gtaggtgctt
     7381 tgatagatgt tggtctaaaa agaagagctc cacaagatat taagtatatg cttgaatcaa
     7441 aagatagatg tcaatcatca gatactgcac cagctaaagg actatgtctt tggaaagtaa
     7501 catattaatc aattatataa aactctcttt aaaataaaaa taaaataaat tatatagttg
     7561 acataaatgg aaaaataaac tattatatat tttataagag agatataaaa ctttatattt
     7621 aaatcctgtg agaaagagag taacttctat gtgttttata gagagctgag gatggtggaa
     7681 gcttggcaaa taagtattag tgaaaagaac tttggaggat actaattgaa tttagtatgt
     7741 tagtaggtaa tctcgcctta agagaaagag tggataagtt tatcttatca attagagtgg
     7801 taacgcggat atatttcgtc tcttggtttt tccaagaggc gttttttaat atatttaatt
     7861 attagtgttt atctggtatg ttgaacattt actaattgat tagctaagtt tatcaataag
     7921 agtggtaccg cggatatttc gcctcttagt attttaaaat actaaggggt ttttttagta
     7981 ttaaaatgat tttatgtgag tctaaattct cagagtcatt caatattaag aatttctata
     8041 aaataaaaat tataatttaa gattgggagg aattaaaaat gaaggaaaaa gtagtattag
     8101 catattcagg aggacttgac acatcaatca taataccttg gttaaaggaa aattatgaag
     8161 atatagatgt tatagctgtt tgtggaaatg taggtcagga agataaaatg gaagatgtat
     8221 atgaaaaagc tttacaaagt ggagcatcaa aggcatatgt ggatgatata agtgaagaat
     8281 ttgtaactga aactatattt aaagccgtaa aagcagaggc taaatatgaa ggaaaatact
     8341 tattaggaac ttctttagca agacctataa tagctaaaaa attagttgaa gttgctcata
     8401 aagaaggtgc aaaatatata tgccatggat gtacaggaaa aggtaatgac caagttagat
     8461 ttgaagctac aattgcagct cttgacccga ctataaaagt tattgcacct tggagaattt
     8521 gggatataaa atcaagagaa gatgctatag actatgctga aaaacataat ataaaggtaa
     8581 cagcaactaa agctaaaata tattcagtag atgctaacct ttggcatgtt tcaacagaag
     8641 gtggagatat agagcaccta gaaaatgaac ataaaaaaga tgtttataaa caatgtgtag
     8701 acccagaaga tgcatgtgat gtagctgaat atgtagaagt ttactttgaa aaaggtgtac
     8761 ctaaaaaggt aaatggtgaa gaattatctc cagttgcact gattcataag ttaaatgagt
     8821 tgggttgtaa acatggaatt ggtgttatag atatagttga gaatagactt gttggtatga
     8881 aatcaagagg tatatatgag actccaggag gaacaatcct ttatgaagca cataatattt
     8941 tagaaagtgc tactttagac aaagatacat tacatttcaa acaaatggta tcttataaat
     9001 acggagaact tatttacaat ggactatggt attgtaaatt aagagaatct atagatgcat
     9061 ttatggaaca aactcaagat aatgttacag gaacagtaaa agttaaactt tacaaaggaa
     9121 atataaaacc tgctggaata tttactgaaa atgctcttta tgatgaagga atatcttctt
     9181 ttggaaatag tgaactttat gaccacaaag atgcagaagg atttattaat ttattcactt
     9241 taccattaaa aatcagagct atgaaagctg gaaaataata aaataaatta tttaagagtg
     9301 tataaaaact atttttaggt ttttatatgc tcttttttta tttgaattta ttaacttata
     9361 cagtaaatag tataaaaaaa tatattatag ttccaaatgg cttaataatg tgaaaatata
     9421 agacctaaaa tataaatttt ataaatacaa tagttattga ctctctcatt gtaagagggt
     9481 ttatactgaa tctaatgaaa atttatagtg aggagataga ttatatgaga aaaataattg
     9541 aaattaaaca tcattcacat gattatgagt gtatgtggaa tggtatagaa gatttatata
     9601 taaataaaac cggtgaatgc cttccaaacg attttttctt tttactgtca ggttttggtt
     9661 cattttgtta tatgaaaaca aataaagctg atttgaaaag aatggtagct ttgggagatg
     9721 gaaggacaaa acagatgtat aaatttttag caccaattgt tggatttgaa tacaaacatc
     9781 atgagtttaa aaagtatgag caagcattaa agaaagcaaa atctgaaatt gatgatggat
     9841 atccagttat tttaggagct ttagatatgt attatctacc atattataag aaactatatc
     9901 ataaagaaca tattcctttt cattatatat taatggtagg atatgatgat gaaggaatat
     9961 atctgtatga ttgtggtaga acagaattat tacatctttc ttatgataaa ttacgtgaaa
    10021 gcatgaattg caattcccct ggattaagca aagaaaatac tatttgcaca ataagaatga
    10081 atacttcaaa gaataaatat aacattgcta gagaagctat tgctataaag cgtgatatgt
    10141 ttttaaatcc acctaaagga tttttgggat ataaaggttt tgaaaagttt attaaagagt
    10201 taccacagtg gaacactgaa ttggataaag atgattatga taagattttg accaatatgg
    10261 ttatgttttt tggaacagta ccaacaattc caaatgcaat caaaggaatt aaagaaccag
    10321 atactgttca atttaaaggt ggatttgata aaatgagtag aatgctcaac agtattgggg
    10381 aggaatacaa aaacgactct tttatacaag tagctgatat ttttgataaa actgctatta
    10441 taattgagaa aatatccaat attataatag attacttaac tcaaacatgt gatgatacag
    10501 aacaacttcc gttattgttc tctgaggtct tagagcatat gaaaacagga tatttaatct
    10561 tggatgtata aaaaatatat tgttgaccct aacattacgt aatagtgtaa tatgaaaata
    10621 tagcttgaat atattagtag gtattgatgg ctgtaaaatt aaaatttgag gtgtggatat
    10681 gagaacagtg aaacaagttt ctgatttaac tggaataagt gtgagggcat tacattatta
    10741 tgatgaaata ggtcttttaa aaccaaataa aattacagat gcaggatata gactttatga
    10801 tgatgaatct attaaaactc ttcaacaaat tttatttttt aaggagattg atattccttt
    10861 aagagaggtt aaggaaataa tgtcaagcca atattttgat aaggttgaag ctctaaaaaa
    10921 tcaaaaaaaa ttacttattt taaaacgaaa gagactggat gaattgatag agcttataaa
    10981 tcaaactcta agaggagaag gaaatattga ttttaaagag tttgatatga gcgagtattt
    11041 tgatgtttta gaagaattta aaagagagca taggaataag gtaattaaaa tttatggtag
    11101 tgtagaaaaa tacaatgaat atatagaaag agttaagtct aatgaagaga aaattgctaa
    11161 aatggctata aaacaatatg gaactattga gaagtttgct aaagctataa aaacaaattt
    11221 tagcagtgat atattgaatt taggagaaaa atttgataga tataaaaatg attgtttaaa
    11281 agaaaaacat ccaaaattaa aagaattata tagaaagctt gtagaagatt taagtaggaa
    11341 tccttcttca acagaccttc aagaaatagc aaaagcgata acagatatat ctaaaaaaga
    11401 ttatgaaatt ttctctatgg atactggtga tgacaactgg tattatatgg ttcaaaatta
    11461 tctagtaaat cctatgtgga tagaagaagt agataaaaag tatggaagtg gagcaggtaa
    11521 gtttattgga caagtactaa agatttatct aagagataga aaaccaaaga taaatacatt
    11581 gtatgaaaaa cttgtagaag atttgagtag agattgttct tcaagggaag ttcaaagcgt
    11641 tgttgaagaa atagacaatg aaatgaaaag aagtaatgaa ttttataaaa tagacaatgg
    11701 tgaaagatac tttgattata tgtcagagct ttatttacaa gattctaact atataaaagt
    11761 aactgataag aattatggtg atggtgcatc taggtttatt ggagaagctt ttaagattta
    11821 ttttgataat aataattgtt gaaaatttgg aagtaaaaaa tatgtaaact ttataaaagc
    11881 acaaaaataa ggatgtagat tagctccatc tatatcctta tttaaagtta ttaaaatata
    11941 tagtttacat caaacttaac actttcaata atatttccca aatatgtttt tatactttca
    12001 acttcttcta aagattttaa tttattttca gtataaattg ctttgtccag aatattaaac
    12061 cttaatccta tttcatcaaa gtactcatga tttaaaactt tgtaccaatg gtttttttct
    12121 ttattccaac tcgtagatat tgaatcagct tctttttgag ctttttccca atcttcattt
    12181 tctatagatt tttctaaaac agatatattg tctttatatc cttctgtaaa atcatatagt
    12241 ttattgttaa cataaaatcc aaataataca aataagatag tccatagtat aacaaatgtt
    12301 gtagatttca atattaatca ccctttttat tttgaataaa tattttatca ttttcatcta
    12361 aaacacaaat aagaacatct ttaaaagttt ctatgtgatt agattttaaa atacccatta
    12421 accagttagt atctttttga gctatgtcta gatttttatt tattattcta ccatccagta
    12481 taagtgggat aggtaaatga ttaaaagctc ttggaggagt actattgtag cttgatgctg
    12541 gtacaacact tagattacca tcagtctcca aaatagcata ttgtacatct tttaggttaa
    12601 agtatccttg aatcctaagt tgttctaata attcatctat actaattctt tcttttttaa
    12661 gctctttata tacaattttt cctttatcta ttaatacaga aggtttacct gataaaaatg
    12721 aacttaattt tctgcttttt aatgataaga atgaaattag agtctgcatg attacaagtc
    12781 cagatattgc tgcaacacca tttatgattg gtatattatt attttccatt gggacagatg
    12841 caacttctgc aattaatagt gttataacta aatcaaaaca gttcatttca gctatttcac
    12901 cttttcccat gactctaagt gcaattagca ctgttatgta taatataata cttcttatta
    12961 atactactgt catatctatc aattactcct ttaaaaaata tttacactta gttagtatat
    13021 cctatataaa aaatttaatt caaagtataa aaaaccttaa ataattggat tcaactattt
    13081 aaggtttttt tgataatttt aatattaaat tttttatgga ttttctgtta cttttattta
    13141 ttttaataac agattcaaca aaactctttt tatctcctaa ttttaagata aaat
//
LOCUS       DAEMIK010000125        13024 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEMIK010000125 DAEMIK010000000
VERSION     DAEMIK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.126"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            46..1383
                     /locus_tag="KPK87_002918"
     CDS             46..1383
                     /locus_tag="KPK87_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2539047.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1719..2288)
                     /locus_tag="KPK87_002919"
     CDS             complement(1719..2288)
                     /locus_tag="KPK87_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG2539048.1"
                     /translation="MKKRLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(2640..3653)
                     /locus_tag="KPK87_002920"
     CDS             complement(2640..3653)
                     /locus_tag="KPK87_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2539049.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQVNVY
                     IDN"
     gene            complement(4265..5167)
                     /locus_tag="KPK87_002921"
     CDS             complement(4265..5167)
                     /locus_tag="KPK87_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG2539050.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(5294..5413)
                     /locus_tag="KPK87_002922"
                     /pseudo
     CDS             complement(5294..5413)
                     /locus_tag="KPK87_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..6621)
                     /locus_tag="KPK87_002923"
                     /pseudo
     CDS             complement(5959..6621)
                     /locus_tag="KPK87_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(6781..7318)
                     /locus_tag="KPK87_002924"
                     /pseudo
     CDS             complement(6781..7318)
                     /locus_tag="KPK87_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
     gene            complement(7391..7804)
                     /locus_tag="KPK87_002925"
     CDS             complement(7391..7804)
                     /locus_tag="KPK87_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2539051.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            8297..8935
                     /locus_tag="KPK87_002926"
     CDS             8297..8935
                     /locus_tag="KPK87_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG2539052.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQDSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(9543..9722)
                     /locus_tag="KPK87_002927"
     CDS             complement(9543..9722)
                     /locus_tag="KPK87_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2539053.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(9948..10481)
                     /locus_tag="KPK87_002928"
     CDS             complement(9948..10481)
                     /locus_tag="KPK87_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG2539054.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQITVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            10798..11148
                     /locus_tag="KPK87_002929"
     CDS             10798..11148
                     /locus_tag="KPK87_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539055.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYNW
                     RNKVFSILQRLIRLEKSKNELYMYEALVSASFLWLIMIKNIVLPPIKHKNIINIHMQK
                     ILRYIEKYYCFTLY"
     gene            11117..11436
                     /locus_tag="KPK87_002930"
                     /pseudo
     CDS             11117..11436
                     /locus_tag="KPK87_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(11738..12310)
                     /locus_tag="KPK87_002931"
     CDS             complement(11738..12310)
                     /locus_tag="KPK87_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2539056.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(12372..12635)
                     /locus_tag="KPK87_002932"
     CDS             complement(12372..12635)
                     /locus_tag="KPK87_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG2539057.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(12635..12856)
                     /locus_tag="KPK87_002933"
     CDS             complement(12635..12856)
                     /locus_tag="KPK87_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG2539058.1"
                     /translation="MGYLKEDKQGYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         4633 a         2203 c         1492 g         4696 t
ORIGIN      
        1 taaaacttag tttccagaat cttaattaat agaaaggtat attaaatggg cacttattct
       61 ttaatctcta caagcataaa ttttattcta atattaatca atattttcat agcctttttt
      121 ctttataagt tgacttccta ttttatagac tttaggaatc attggatttt caaaatcata
      181 ttgattttgg gctactctat tattagtcaa atgataatat ggctttccga ccccgtaaat
      241 attttgttta cactattcgg atattttata ctacttataa tatgtagcaa aagcaaatta
      301 attcctataa tatcagttgt catgatactt tatcctatta taattgggct taattttctt
      361 tttataaaca accctatata tgaggaactt atacactcaa aagagtatat tttaatactt
      421 tcaatagtcc agataatttc tgctctaatt aaattattat tttggttttt aatacatatt
      481 ttcctaaaga atagattggt caatataaaa caatacttat caaataaaat atggattttt
      541 attgatatgg tgtgtatatc ttcattttta tcaatattaa ttgctattat tctgccacct
      601 actaatttaa tccctatggg tagtaataag aatattatct taggcaactt accttatggc
      661 acttatctaa tcatcttgtc agcaattctg gcaaatatcg gtgtgatact tcttttacag
      721 ccattgattg aaaatgtaaa gatgaagata gaaaaacaag caaatcacct aaaagaaaaa
      781 tactataact tactagaaaa tcaacaaatt caaatacgca aaatgcgaca tgatatgaat
      841 aatcactttc aaatgattga atcgtattta gaaattgaag atattgctgg agctaaaaag
      901 tattttaatc agttaaaaat aggtataggt actcttagtg gcaaacaatt ttgcaaaaac
      961 caagctctta actcaatttt aaatcttcgt catacaagtt tggaagaaaa tggtgttgat
     1021 gtgcacttta atatagatat agacaatcac ttgggtatag aacctataga tttatgtact
     1081 attttttcaa attctttaga taacgctata gaagcatctt tgaaaattcc tgatttgtca
     1141 aatagaaaag tggtattaaa ggcacgttgt gagaaggagt attttagcta tttattgaca
     1201 aacaacaaag taaatcctat caatagaaaa caagaactat ttatttcaga taaaaaagat
     1261 agtaattctc acggatatgg tattgagaat attagagaaa ttgttaataa gtacaatgga
     1321 aaattggata tttcttatac tgagagtgaa ttttctttat ttctttatat tagattattt
     1381 tagtctaaaa ttagattatc ttatataata caaattagat tgtttgatac tatgtaaaat
     1441 tataataact tataattaaa tatttacaca tatattcatt aacttaaaat ttaatcaaat
     1501 gtgatatttt atttacctta gtaaaaatgt gtgtgtctca aaataaactt tttagttgat
     1561 gaggcactct tttttacatg aaatcaaata tatttttatc ctttcaacaa tgaaaaaggg
     1621 atttatctct ccattttgaa acaagccctt tttctattaa ttactttatt aaaaaatata
     1681 atacgatata taagataaat atctaattgt acctatttct aattatatct attagaatac
     1741 aacacctttg ccttttcagt aaactcccta acaaaatcta gtttactatc tgtataagca
     1801 tcacgattat gctcatactt cttccaaagg tccaacttaa gtttttcata ctcctttgca
     1861 acatctgggt ggtcaattaa gtaatctcta aaaaacaact catcattatc acctacatac
     1921 cttatatgca aatgaaaaac tcgttcttca aacccatcta ctgtataccc tttattaagt
     1981 gaaagcctac tactgctctc agacatacaa atatatccat tcgactcaat caaatcttta
     2041 atatctttca tattataatc aggaattatc tcaatcagta tgtcaacaat gggcttggca
     2101 taaatcgtac tcacagaagt acttccaata tgacttattc taacaatttt ttgcattggc
     2161 aatattccag ttagtctata ttgttcttct ttaaaccaat ttttccaaca ttctttgtga
     2221 gtagtcaaaa atataggaaa caagttccat aactcctcta aagtcatatc ttccaacctc
     2281 tttttcatta gttacctcct tttaaatttg tactttatat tttttctttt tcaaatttac
     2341 ttagttaata tttaacttta ttttttatca aatcattttt ctaattatgt aaatttttct
     2401 aaatttagtt tttaatgtat tataacagat ttttaccctt cttacacttt tgcatttctt
     2461 taaatattag aacattttta atctacaatt gtagtaattt tttctttacc tgtatttact
     2521 ggcattggag cttctaaaga ataattcatt ggctttaata taattaaagt aaatttaaca
     2581 tatgtgaaag atacttatta aaattattta tattaatctc ttgttaaact ttgtattttt
     2641 taattatcta tataaacatt aacctgatat gtaggttttg aattagttct gttttcaata
     2701 ggtgtttgtt ttgaaagtgg ataaaatatt gatttagtag taattaaatc aattttaaaa
     2761 tatgtaatag aagataataa acataaaata actgctatca aaataccatt tctgtacttt
     2821 tcattaaaca tactaaacac tcttttttta atatctgact ccttatgtaa tccagccaca
     2881 aatttatttg aactatttga accttttctc attgcactaa ttattgtcaa tgcataatcc
     2941 tgcttctctt ctatcgtaca attcttcaaa actctttcat cacaataaaa ttcacaatcc
     3001 aaatctataa acttctccat tatatacaca aaaggattaa accaataaac acattttaaa
     3061 aatatcacca aatacttaat ataaaggtct ttactcttaa aatgcatcaa ctcatgccta
     3121 aatatccagt ttaactcttt taatgtataa tcacatggtg gtaaaatgac acacgaatta
     3181 aaaaatactg gactttcaac ttcatatgaa tatttaagcc gtattttttt atttatctta
     3241 agctctttta ataaattttc ataaagattt tttatcttat catcctcaac atcataagac
     3301 aaatcattta tcatattttt aagcttaata tatttcaata aagtataaac taaataaaat
     3361 atcgtaatag caatccaaac agagactaaa acagtactat atacaataga aatactatta
     3421 aaattgccta aacttatctt attaatatta ccattatatt ttgactcatt aaatataaaa
     3481 gtataattca taaatggtaa tgatagctta ataatcacaa taagccatat atagtaatta
     3541 aattttttac tgaaaatctt aaataaagtt attctaaaga ctaataaaat aactataaaa
     3601 atacaactta ctaaaattgt tctaaagata tcatttaaaa atgaactaac cacagatact
     3661 cacctttctt atacttttat atttatataa tcatctacac gaatactaaa ctgatatgta
     3721 ggtttttaat taactttgtt ttcaataggc gattgtcttg aaagtgtttt aaaagttgat
     3781 ataacaatga tttaggagta ataaggtcaa ttttaaaata tgtaataaaa tataataagc
     3841 atgaaacaac tactatcaag ataccatttc caaactctat aagcaaaagg ggctaataca
     3901 accttaaccc cttttaatta ttttcattta taaataaatt ctattcttcc caaaatatcg
     3961 taattaaaat attaattaaa atatcccaat gagaatatct gaaaaatctt ctacctattt
     4021 tttcaaaccc tttattccca ttcctcatac aatagtgcaa cttttagcac agtataaata
     4081 taccattagt ccatataatc tcattaacaa atatatacaa ttattttctt caaactcttt
     4141 tatatctaag atatcacatt ttcattttct tatattaatt ccttaactct ctttcaaatt
     4201 accaattttt aattatcaaa tcttcattaa atattctatc attattaaac attcatcatt
     4261 atccctaaaa aatatgtgcc gtatgttctt tacacaaatt ttccatctta tcatacacag
     4321 caccactatc tctctcatct gtcatagtca caattacatc acttggtcta agaattgtct
     4381 tacctctagg aataatttct tctccacctc tttgaattgc taccaaaaga caattttcag
     4441 gccactgtac atcctgaatt gccttgttcg caacaggaga gccaaacata acagagaact
     4501 ggtctagtat cttttcgccc ctctctttat gtacaggttc tccatttttt tcaagtaacc
     4561 tctcaagaag actttcataa ataggtttag acttcataag tgtagccaca atatatgcca
     4621 caattgaaat tactgatagt gaaagcattt gactaacaga acctgtcatt tcaaaaatta
     4681 atatgatacc agtcaaaggg gcacgcacaa tagctgtaaa atatcctgcc atagccaaaa
     4741 gcacaaagtt atttacataa tcgggattca ttccaaaaac tttaacacct accataccaa
     4801 atattccacc tataaatgct cctaaaacaa gcaatggaaa aaatattccc cctggtgcac
     4861 ctgacccaaa actaacagcc gaaaatataa aacgtataac aaaaatcaaa acaaccattc
     4921 caaaaatata ttcatgactt gtaagttctt caactaaatt gtgtccactc cccagaacat
     4981 caggcataac aatcccaaga ataccagcca ttataaatgg aatcaacagt tttccaaagg
     5041 taccaatttt aggaatcttc ttgtataaag actgcacata tagagtaaac cagttgtaaa
     5101 atgcccccat aactccaaga ataacaccta aaatcactat taatccataa taatcttgtg
     5161 gcaacacatt tccaatctga acctcccatg actaaagtca cagggttcct gcttcataga
     5221 attttgcata ctaaaatatg tcttactaat tctccacagg ctatccccgt agttcctacg
     5281 gttcttacat aagttattta ttttgaattg ttattagtct taatccttct tttaatatat
     5341 ttatacttgc atttatatct ctatcgtgag tttcattaca actaggacaa atccattctc
     5401 ttataataat gttaacaaat gtaattattt aaaacaatta tttagaaggt tttatattta
     5461 attttaaata acatatttaa gaaccgtaag tcttaacttt tatcattttt gaggttgtcg
     5521 ttcacataag ttcgctacta cctatgcagt tctcttatga acttcttaca ctttcatgca
     5581 agcacagact atatcttatc cttcggcatt atccgttaag gtctacccac ttccactacc
     5641 aatagcttgt agtgtacttc cctcaagagg aatagtcgtt gaagtttctc ctattcggag
     5701 tttacctgct gattgcccat tttaaagtac ttaggattta accttatacc atctaaccaa
     5761 ttttttctac tttcgtaact ttcacactta ggtttatttc atccttatgt tttagtttgg
     5821 ttagctttag gggtttccag caattcaagt agtattggat aggttctatc ctatctctac
     5881 atataggttt ccctatatgc tgactatttt agctactaac tcatgactaa agtcacaagt
     5941 gtgcgtagct attttttaat caaactggaa tacagactcc attcctagga aattggctga
     6001 aatatagtct gctgtcaaag atgctgtcat cacagaaatt aaaacagata ctgaaaagtt
     6061 tttatggact tcttcaagtg aaaacataac acccgccagt ggtgcatgaa aggctgctga
     6121 taatccagca cttgccccac aggttaacag gaatttttcc tctgtcttcc ctcggtcaag
     6181 agctctagaa atacctttac ctgccatagc accaagctga atggaaggac cttcccttcc
     6241 caaagcaaga ccacccatga gtgataagaa tcctccaata aattttgttg gcaacactcg
     6301 ccaccatact tgttttattt tccctgtcat ttccccctca agctgaggga taccacttcc
     6361 agaaatcatt ggctcccagt ccaccaactt tgatacaatc catgcaagta ctgcaagtac
     6421 tgcaaaccaa gctattattt ttattgtaga accttctaca aattttaaaa tttggctaag
     6481 ccaagtacca gcctgcccta aaagtatcct gtataaaagc acaatcaaac cagccacagc
     6541 cccaaccata agtccttcac caatcaaaat tacctgaaag tgctctgccc gtttgataat
     6601 atgagatgta tcctttttca tactattcct ccttcatcca ataattctac acaatctgaa
     6661 tctccaatga ctaaagtcat gagtgtgtac agatattttt taatcaacaa aaatagattt
     6721 ttacttacta aatataagaa aataattgct caatttctca ataaatgtga atttattctt
     6781 ttaggtattt attctccaca gtcgtgcttc ttccagcaaa atatttatta attcttgctt
     6841 tctctgtgta tagattttac gattgtcctc aaactgtaat gctaattttt gtttatgact
     6901 atcatagagt tttgctttgt ctgtaaaagt atttagataa tctcgaaaat tgatataatt
     6961 attccatgca acactattcc attcaacaac atgaatatgg tgagtacgag tatttttctc
     7021 aaaatctccc atagcaaaaa gtaattgttt tggtacatcc tgacctcgaa aaacgattcc
     7081 acattgctct aacctttcga ggtataattt agccttcctc taaagaagca acacctacaa
     7141 caatatcaat aataggcttt gcgctaatac tgggaataga agtgcttcca atgtgttgaa
     7201 tatcaatggc agtcttatcc catatacttt tcaataatat aatgacatct tttgcacttt
     7261 tatcccatat catctgatgt tccaataatt taaccatacc acattttaaa cctatcacta
     7321 tataccacct caatataatc cacctttata tatattacaa ttttttctat cttgataaat
     7381 agtgatttag ctatttcact ttttcttgac actcgctaca ttccctatca tgaatagata
     7441 taattcctcc acatttagaa caagtatatc tctccttctg cttttgcata aacttctcta
     7501 aaccgtactg ctgaacaaat ttactatttt ccataaggct tgcttgatat ctcttgttat
     7561 aacttttttc aagatttttt atgagtttac aaggaaaatt gaaacattca aaacaataaa
     7621 acaacccttt ttgacagaca caatccttta ttttacactt acggcagtgt tctggctttc
     7681 ctgtatcgcc attcaaacaa ccagcacatg gctttttgtg ataacaatgc ttatagcaaa
     7741 ccatacaatt cattccacaa ggagcaaaca taatcgtatc aatactctct tttggcattt
     7801 tcataacatt ttactccttt caaaaatcca atttgtcgaa ttgttatata ttcatattgt
     7861 aacataaaaa atagctaaaa aattcatgca ctttcattgc caataaattt tcaaggaaat
     7921 atgaaagttt aaaactaaga ataaaaagag ataaaaagga agctactaat aaaaatattc
     7981 aaatacaagt aatcaatata aataacttaa ggtatattta aaagtatatg atattaccag
     8041 cagttttaaa tacaaatata accacacatg cctcaaaaac acttattaca gtatcttaga
     8101 aacttagaca atacataaga ttcattaata aaaaagttct caatatagtc attaataaaa
     8161 acgttctcaa tatagatata tttaaccatg ttttgaatag cagataatta tatgattaaa
     8221 aattaaatat gacatagcgt ttgtggaata taacacctaa attatgttat aataataaaa
     8281 caggagtgtg atagtgatgg atgaaaaaat attagagtta ttacaggaaa tgcaaggtaa
     8341 tattagtaat atgcaaggta gtatcagtgg tatcgaaggc actattagcg atatgcaaag
     8401 caatatcaat ggtatcgaag gcactattag cgatatgcaa agcaatatca atggtatcga
     8461 aggcactatt agtagtatgc aaggcaatat caatggtatc gaaaacacta ttagtggtat
     8521 gcaaggcact attggcgata tgcaaggtag tatcagtggt atgaatggca ctattagcga
     8581 tatgcaaggc agtatcagta gtatggatgg cactattaat agtatgcaag acagtattac
     8641 tgatatgcaa gttgaaatta aagaagtcaa tagtaggtta gacaatatgg aacctgatat
     8701 taaagactta aaaactggac aagttgaaac tagtagtaga cttgatacta ttcagagtga
     8761 tgttacagaa attaagtcag atgtaaaaat taacagaaat acattagata cagtgattaa
     8821 acatacctct tcattagtaa aagatacatc agatattaaa aatgatttaa ataatataga
     8881 gatagtcaca tctaagaatt cgaatgatat tgctaaatta aaatcagtta aataatatac
     8941 tatagtaaaa aatacctgaa aatttcacat acttctttaa agttatacca aattttatac
     9001 agaaatactg attatccgac aacacacttt acttataaat aatttattaa aaaaatactc
     9061 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     9121 gattggacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     9181 tcagcctaaa ataaaagtcc atctttgatt aaaacacatc cccttatcac ttataaaagg
     9241 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     9301 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     9361 atttttcata ttttccaata cttaatctat tttttataaa taattctttc atttctaact
     9421 cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt gtatggttct
     9481 taatacttaa tgagtagctt aagtatcaaa cattataaat tttcccctta catttaacac
     9541 tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg atgaaataca
     9601 atacatgaaa atacaatatc ataactcttg tctaaaaata gaagttcttc aacataacct
     9661 ttcacaaaaa acatattatc taagtatttt ttgtctgctc cttgttttcc tgtttgtagc
     9721 atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa taaacttcca
     9781 aaatacaagc tctaaaaact acctctaaag ttgtataatg tttactagga ataaggttgt
     9841 ataacatata gtttaaataa tcatttttta aaattgcaag ttattgcttt caaaattaga
     9901 tgtttgtata tcaaataaat gattaagaat ttctaaattt aatggcttta taatgcactc
     9961 cctttctttg ggataaaata atgttgcaca gatacttttt ctaaagataa caattttatt
    10021 tttctatcaa ttccaccagc ataccctgtc aaactcccat ttgttcctac aacacgatga
    10081 catggaacaa taatagagat tccattatgc cctactgcac caccgattgc ttgtgctgac
    10141 attttagata ttcctttttg ttgagcaact ttatatgcaa tttctccata agtgattgtt
    10201 ttaccatatg gtatttgttg taaaattttc cacactgtaa tctgaaaagg agtacctacc
    10261 atatggagtg atggtgtaaa attgggttct tttcccgaaa aataaatatc taaccatttc
    10321 tttgtctgct cgaaaattgg caattctttt tcttgatgtt cttggtcaag attataagca
    10381 taatactttt gattctcaaa ccaaagacca gttaacccaa tattatctga tgctaaaaga
    10441 atatttccaa ttggactatg gtaatagcta atatattgca tacaattcct ccttatattc
    10501 tgaaaacttc actcaaatca ttagacactt cttttctatc tgccattatg taaaaacata
    10561 ctaatataat ttccttctaa ctacactttc gttataatat ctatcctcat atcatattag
    10621 ttttatattt acctgcatct gttcatcctt ttttcccttt ccctttgatg aatataaaat
    10681 tcatacattt aagcttccta ctgtattaac ttttatagaa gtgtatatta taattttatc
    10741 accaaataga ccaaaaaaat atcgatatag catcaagtaa tatcataata ctttcaaatg
    10801 ggaaaagtaa gaataggctg taaaaaagaa aagtattttt atgttagaaa tccagtaaaa
    10861 tcgttagtag atagaataac agaaaacgaa caatttgcta tttatcattt tacacataaa
    10921 tataattggc gcaataaagt gttttctatt ctacaacgat taataaggtt agaaaaaagt
    10981 aaaaatgaac tgtatatgta tgaagcatta gtttctgctt ctttcctttg gcttattatg
    11041 ataaaaaata ttgttttgcc accaataaaa cacaaaaata taattaatat ccatatgcaa
    11101 aaaattttac gttatattga aaaatactat tgttttacat tatattgaag ggtactattg
    11161 tgaaaactta attttggagt actatcaagc agtgataata tcaataaatc agagtgttcc
    11221 agaggatttg aactaagttt aaatactata ccttgtaaat attttactga gttttgttta
    11281 ctaaaagcaa ctcaatttct aaaaggcaca aatgagctaa taagtgacat tgctataaaa
    11341 gttgtttttc atcaaataat ctattttagt aaatgtttta aagaaaaaac gggatattct
    11401 ccaaaagtgt acagaaatat aaaaaaagag atatgatttt ttatttcttt tgattatcca
    11461 aagagtaaat aaaaaatatt gtaaaaaact tactaacaac gacaatgctt tgaactatgg
    11521 taatactttg aacttttgat tggacttttt ttgctaaaat aatatattta aatagcaaag
    11581 taatctgttt aatcaataaa aaataagaga taaaagaaat caactaaaac ttttacctct
    11641 tattttgata gtttatcaga gctaatattt acactctttt tatttttgat acttcattaa
    11701 aaatgatgct tattctccat tcactttcaa ttcttcatta aaactgatgc ttgcactctg
    11761 taaactcata aacatccatt aatgcttcac caaatctttg atagtgaact acttccctct
    11821 cacccaaaaa tctcaatata tctttcaaat cagggtcatc agttaaattt attaattgat
    11881 agtacgtagc caatgctttt tgttcagcag ccatatcctc atgtaaatca gttaccgaat
    11941 cgccaaagac attaatataa cttgtagtaa agtttactcc attagaatct actggctgaa
    12001 gaccatgtcc aaaattagca taattactac caagtccagc agccttaagc tcttctggag
    12061 tacaattgcc tattaattga tataacactg aagaaattat ctcaacatga gccatttcct
    12121 ctgtacctat atcagttaaa agtgcacgag attttccagt tggcatagta tatctttgtt
    12181 gaagatatct agttgcagct gccaactcac cattaggtcc tcccaactga gtcatgatat
    12241 attttgccat tctagggtct gcttgtctta tgttaactgg atgttccagt gttttttgat
    12301 aaatccacat attatcttac tcctccttaa tatatattta ataataaatc taattttaaa
    12361 gtttaataat tttatttata aaatgatttg tcccatggcc aaggttcaga tatccatgac
    12421 caaggatatt cacttgattc atatccaaaa ttagttaatg ctccatattt agattcataa
    12481 tcacacacag cttgtgtaaa tttatcgcag tatctgttgt atgaatcaag agctttctta
    12541 tcccttggat tgttatctaa atataaattt aattctatac aggcaaattg atattctgaa
    12601 attctctcta aaagttctct tctatttaat gacattagta catgttaccc ccttttttag
    12661 ctctaactct atttttagga tttgcatata gccaatcatt atagttattt gactctatta
    12721 aatctaattc aggaaataaa gtacctcttt ttaatgctgt ttttggattg tatgtgtcat
    12781 cataaatttg acgattgatg tatggtctag caaattcaca acaatagcct tgtttatcct
    12841 cttttaaata tcccattaat tatcctcctt aatttttaga attttaaaat agcattattt
    12901 ttaaaaattg tatactggtt ctatattata actggtagtt ttaataggtc tttaataagg
    12961 ttaaatgatt aaataagtat ctttttttct tattttacct ctaaaatttt tctaattaat
    13021 cttt
//
LOCUS       DAEMIK010000126        13021 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEMIK010000126 DAEMIK010000000
VERSION     DAEMIK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.127"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..789
                     /gene="ispD"
                     /locus_tag="KPK87_002934"
     CDS             76..789
                     /gene="ispD"
                     /locus_tag="KPK87_002934"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG2539059.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            786..1271
                     /locus_tag="KPK87_002935"
     CDS             786..1271
                     /locus_tag="KPK87_002935"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG2539060.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    1366..1574
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1638..3353
                     /locus_tag="KPK87_002936"
     CDS             1638..3353
                     /locus_tag="KPK87_002936"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2539061.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLVSQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3475..4920
                     /locus_tag="KPK87_002937"
     CDS             3475..4920
                     /locus_tag="KPK87_002937"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2539062.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            5360..6841
                     /locus_tag="KPK87_002938"
     CDS             5360..6841
                     /locus_tag="KPK87_002938"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG2539063.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            7062..7217
                     /locus_tag="KPK87_002939"
     CDS             7062..7217
                     /locus_tag="KPK87_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539064.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            7362..8759
                     /locus_tag="KPK87_002940"
     CDS             7362..8759
                     /locus_tag="KPK87_002940"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG2539065.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEYKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            8744..9157
                     /locus_tag="KPK87_002941"
     CDS             8744..9157
                     /locus_tag="KPK87_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG2539066.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            9263..10021
                     /locus_tag="KPK87_002942"
     CDS             9263..10021
                     /locus_tag="KPK87_002942"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG2539067.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLKEVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            10034..10771
                     /gene="rlmB"
                     /locus_tag="KPK87_002943"
     CDS             10034..10771
                     /gene="rlmB"
                     /locus_tag="KPK87_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG2539068.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            10774..11301
                     /locus_tag="KPK87_002944"
     CDS             10774..11301
                     /locus_tag="KPK87_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG2539069.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            11366..12013
                     /gene="sigH"
                     /locus_tag="KPK87_002945"
     CDS             11366..12013
                     /gene="sigH"
                     /locus_tag="KPK87_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG2539070.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            12087..>13021
                     /gene="tuf"
                     /locus_tag="KPK87_002946"
     CDS             12087..>13021
                     /gene="tuf"
                     /locus_tag="KPK87_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG2539071.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEV"
BASE COUNT         5415 a         1351 c         2591 g         3664 t
ORIGIN      
        1 ttaatcagat ggttaaaata aaaatattaa aagttttaat aaatgatggt taaaaataaa
       61 atacgaggtg ataccatgta tagtgttata attgttgcag cgggaagcgg aaggagaatg
      121 aacttagata taaacaaaca gttcataaaa ttaagagaaa aagaaattat tgctcataca
      181 attcaagttt tttatgaaaa tataaatata gatgaaatag tggtatgtat aaaaaaagaa
      241 gaagaggatt tttttaaaga aaatataata aataagtata actttaaaaa tataaaaata
      301 gcatatggcg gaaaagaaag acaggactca atatataatg gtttaaaaaa attagatgaa
      361 aattgtgata tagtacttat ccatgatgga gcaagaccat ttgtcgacca tagaataatc
      421 aacgaatcta taaaggtagc taaagaaaaa aaggctgtgg ttgtaggtgt tccagtaagt
      481 gatactataa agatagtgtc agatggaaca gtacaagaaa ctccagaaag aaatttgctg
      541 tgggcagcac aaacccctca aacctttgaa tataatctca taatagatgc ttatgaacaa
      601 gcatataaga acaattatta tggtactgat gattcaatgt tggtagaaaa tataggtcaa
      661 agtgtcacta tggtaatggg ttcttatgaa aacataaaaa taactagtcc agaagattta
      721 aatatagcag aacaaatatt aaatatggaa aaaagagatg aagttaacag tagaaggaga
      781 attatatgag aataggatta ggatatgatg tacacaaatt aacagaggat agaaaactta
      841 taatcggtgg tgtagagata ccacacgata aaggtttgtt gggtcattct gatgctgatg
      901 tattgataca tgcaataatg gattctattt taggtgctct agcattagga gatataggga
      961 agcatttccc agatacagat gaagaatata agggagcaga tagtatgaaa ttattagaac
     1021 atgtgtataa tctaataaca agtaaaggat ataaaattgg taatatagac agtactataa
     1081 ttgctcaaag ccctaagatg gctccatata ttgagagtat gagaagtaat atatcaaaag
     1141 ttttaaacac agacattgat aatataaaca taaaggcaac aacagaagaa ggtcttggtt
     1201 ttacaggagc aaaacaggga atagcttcac aaagtatttg tttattattg ttgacaagtc
     1261 agaataatta atatattatt agtaataagt ttaatggata tcaagtaata ggcctatata
     1321 gaatatctat aatgtatgaa tttaagtgaa attaatataa atataaaatt agctatgata
     1381 aagaaatagt aaaatgatga aatcttcaca gagagaaaac ggtggtgaga gttttcaaga
     1441 ggaagtcttt gaacctgtct tttagcttaa aggtataaaa atctttacgg taggaaccgt
     1501 tatattccca aaagagagct attcatatag ctaactagag tggtaccgcg gaaattaaac
     1561 ctttcgtctc tatacttgta tagagatgag aggtttttta tattttcagg aagttaatat
     1621 tcaggaggaa tctattaatg aaaatgtcaa aaatgtttat gccgacatta aaagaaatcc
     1681 cagcagatgc tgagattaca agccatcaat taatggttag gtcaggcatg ataaaaaaaa
     1741 tgacttcagg tgtttataac caattaccaa tggggttaag agtgttcaaa aaaattgaac
     1801 aaataattag agaagaatta aataaaaaag attgtcaaga aatactttgt gccgccttac
     1861 ttccttctga attatggaaa gagtctggaa ggtggactgc tatgggtgaa gaaatgttta
     1921 gactaaaaga cagaacagaa agagagtact gtcttgggcc aacacatgaa gaagcattca
     1981 ctgatataat aagacaagaa attacttcat ataaacaatt gccactgaac ttgtaccaaa
     2041 tacaagtaaa atatagagac gagagaagac caagatttgg tgttatgaga actaagactt
     2101 ttacaatgaa ggatgcctat agttttgatg tagatgataa aggtttagat aagtcatatc
     2161 aagatatgtt tgatgcatat gtaagtatat ttgatagatg tggattggag aatagccctg
     2221 tacaggcaga ttcaggagct ataggaggtt ctacatcagc cgaatttatg gttaagtcag
     2281 aagttggtga agatgaagtt gtattttgta gtggatgtga ttatgcagcc aatgtagaaa
     2341 gagcagaatc ttgtaatcta gtatcacaaa aagaagaaat gaaagaatta gaagaggttc
     2401 atacaccagg agcagctaca ataaaagagt tagaagaatt tttaaaaact tctccagata
     2461 agtttgctaa aactttagta tatgaggcag atggaaaaac agttgtagtc gttgtaagag
     2521 gagatagaga agttaatgaa atcaaagtat ctaatgctat aggttctgtt atagagtttg
     2581 ctcttgctac agatgatgta gtgagaaaag taactaatgc agaagtagga tttgcaggtc
     2641 caataggtat aaatgctgac tatgtattta tagataaaga aattgttgaa caaagaaaca
     2701 tagttgttgg ggctaataaa actgagtacc atataaaaaa tgctaactat ggaagagatt
     2761 ttgaaggaat agttggagac tttagaaatg ttcaagaagg cgacaagtgt atagtatgtg
     2821 gtaagccact tgaaatagct agaggtgttg aagtaggaca tatatttaaa ctaggaacta
     2881 agtactcaga atctatgaat gcaaacttca tagacaaaga tggaaaatca aagcctatag
     2941 taatgggatg ttatggaata ggtgttgaaa gaacagcagc agctataatt gaacaacata
     3001 atgatgagaa aggtataata tggcctttgt cagtagctcc ataccatgtt gtgatagttc
     3061 cagcaaatat gaaaaatgaa gaacaaatat caatagctga aaatatatat aatgatttgc
     3121 aagctatggg tgtagaagtt ttattagatg acagagatga aagaataggt gtcaaattca
     3181 atgattcaga attaatagga ataccgatga ggattacagt aggtaaaaat attaatgaag
     3241 gtaaagtaga atttaaactt agacataaag aagataaaga gataattgac atagaagaaa
     3301 taaatgaaaa agtaaaggca gagtttatca ggaataatgt taggttagga taataactta
     3361 aataagatat tgtatagata atcaaagttg tactttatag acaccatttt atctatatat
     3421 aaggatataa aatggtgttt ttttataaaa caaataaatg aggaggtaat tgaaatggca
     3481 aaaaatgaaa aacaatttgt tgaagaaata acaaagatgg aagatgattt tccacaatgg
     3541 tatacagatg taataactaa gactgactta gttgattatg caccagtaaa aggttttatg
     3601 gttgtaaagc catatggata tgcattatgg gaaaagatgc aagaatttat ggataaaaaa
     3661 tttaaagaaa ctggacataa aaactgttat tttccattat taataccaga gagtttatta
     3721 aacaaagaag cagaacacgt tgaaggtttt gctccagaag ttgcatgggt aacacatgga
     3781 ggaaataaaa aattagaaga aagattatgt gtaagaccta cttcagagac tataatatgt
     3841 acaatgtatg cgaaatggtt aaaatcatat agagaattac catatcttta taatcaatgg
     3901 tgctcagttg ttagatggga aaaatcaact agacctttct taagaacatc tgagttttta
     3961 tggcaagaag gtcatacgtt acatgaaaca gcagaagaag cacaagaaga aacaatacaa
     4021 caattagaag tatataaagc gctatgtgaa gaattacttg caatgccagt tgtagctggt
     4081 caaaaaagtg aaagtgagaa atttgcaggt ggtgaaagaa catatactat agaagctatg
     4141 atgcatgatg gtaaagcatt acaatcaggt acaagtcact tcctaggtca acactttact
     4201 aaggcttttg atattacttt tgcagataga gaaggaaatt tagctaatcc ataccataca
     4261 tcttggggag catctacaag acttataggt ggacttataa tgactcatag tgataacaga
     4321 ggtttagtat taccaccaag agtagctcca attcaagttg ttatagttcc tatagctgct
     4381 aaaaaaggaa atgtaatgga gactgttgat aaaatatatg ctgatttaaa agctaaggga
     4441 gtagctgtag aagttgatga tagagataac tatacaactg gatggaaatt taatgagtgg
     4501 gaaatgaaag gtgttccagt aagagttgaa attggaccaa aagatataga aaataatcaa
     4561 gctatggtat ttagaagaga tactttagaa aaggattcta tgcctttaga aggattagct
     4621 gatgctatat gtgatttatt tgatgttata cacaatgata tgtttgaaaa agcaagaaaa
     4681 catagagaag acaatacatc tatagttgaa aatatggatg agtttagaaa agctttagaa
     4741 gaaaagccag gattcataaa gactatgtgg tgtggagatg cagaatgtga agctaagatt
     4801 aaggaagaaa caggagcaac tataagatgc ttaccttttg aacaagaaaa cttaggtcac
     4861 aagtgtgtgt actgtggaaa agaagcagat agtatggtag taatggcaaa agcttactaa
     4921 aatacattta aataaaaact ggggtagaaa gtatttaata ctttatccca gtttttttgt
     4981 tttttatgag taataaaatg tatttgtaac tatgtcataa gataagtcat agatacttat
     5041 attaaaaaga atataataga gaactatttt taataattac atattaatga aagcaaaata
     5101 tttttgttat atagattttc aataaagcaa acaataaata taaatgaata ttagtaagga
     5161 gtgcttttaa tttttagaaa ataaatgact taaaagaaag atttatgaaa ttaaaactag
     5221 gattttctga tataataaac atgtgtattt ttaaataaaa aggatattat tattagattt
     5281 gattaaaaat aaacttgcaa aacaatgtac tagtttaata ttatagttat attgaataaa
     5341 atatcaggag gaagtaaaaa tgaatgtaag agtaaggttt gcaccaagtc caacaggatt
     5401 tgtacatata ggtagtttga gaactgcttt atataactat ttatttgcaa aaaaaatggg
     5461 cggagaatat atattaagag tggaagatac agaccaaagt agattagtag aaggtgctat
     5521 agagaatatg cttaatgcta tgaaatgggc tggggtcaac catagtgaag gtgttatatt
     5581 agatgatagt ggaaaagtag tacaaaaagg agaatatggt ccatatattc aatctcaaag
     5641 attagatata taccaagaat atataaagca acttttagat agtggaaaag cttattactg
     5701 tttctgtaca aaagaaagac tggatgaagt aagagatgct caaagagcag caggagaaac
     5761 agctaaatat gatggtcatt gtaagaatct ttctaaagaa gaagtagaag cgaatataaa
     5821 agcaggaata ccatatgtta taagattaag attgccagag aatcatacaa ttaagtttac
     5881 agacttagtt agaggagata tggaatttaa tactaatgac ttagatgacc aagtattaat
     5941 gaaaacagat ggatttccaa cttatcattt tgcagtagtt gttgatgact atttgatgaa
     6001 aattacacat gttataagag gagaagaatg ggtatcatct actccaaaac atgtttattt
     6061 atatgaagct tttggatggg aagcacctgt atttgtgcat ttaccaaata tacttaataa
     6121 agaaaagaaa aaattaagta aaagacaagg tgatgtagct gttgaagact ttaagaaaaa
     6181 aggatattta ccagagggat tagtaaacta tgtagcatta gttggttggt ctcctgaaga
     6241 taatcaagaa ttgtttacaa tggaggaatt agaaaaagcc ttctctgtag aaagagtatc
     6301 taaaagtggt ggagtatttg atactgaaaa attaaattgg gttaatcagc actatataaa
     6361 agatggtgat gatgcttatc taacagactt agcaatacca tttttaatag aagatggatt
     6421 tataacagaa gaagaagcta ctaataaata tgatttctta aagagtatga tatcagtact
     6481 taaagagaaa ttacaatatg taaaagaagt tactgaacat gcaagtatat tttttggaga
     6541 taaaatagag gtagaaactg aagagggaag agagttctta agattagaac atatacctac
     6601 tttaatagat gctttgagag aaaaaataga aaaaacagag gtattaaatg ctgagtttgt
     6661 tcaagctatg cttaaagaaa tacaaaaaga gtacaaaata aaagggaaaa atttatttat
     6721 gggctcaaga ataattttga cagggcaaat gcatggtcca gatttaccaa aagtaatgga
     6781 agttttagga aaagaaactt gcttaaatag aatagcttat gtaaaaaata atatattata
     6841 gtcataaaag tccgctaatt tagaagcgga ctttttttgt gttagtattt ggtgaaaata
     6901 tctgcttaag aaaatgaagg tatttaaggt aataaattta atatataaaa agcaaagcaa
     6961 aaatataaat aaaatatgaa taataaacta ataaaatatt aagcatagaa gaagggaatc
     7021 tattatttta gtttgattta attattaagg gggtaattac tatgtatagt aatattgatg
     7081 atgttaaaaa ggaattaaag gagctatgct tagaatatgt tacaatttta gaaaaattaa
     7141 aagatgaaaa aatgattaca gaagaaactt ttgaaaagtg ctcttctcaa aaaaaaatat
     7201 ttttagaaga acaatagagg atggtaatag tattattatt atgtagtttg aaagatagta
     7261 cgaaaaatga tagtaatact attatttatt gcaattttca actataatat aatatataat
     7321 gttaagaaga taatttataa aaatacagtg aggtgatgag tgtgaaagtt tataatacat
     7381 tgactagaac aaaagaagaa tttgttccat tagaagaagg aaaggtaaaa atgtatgtgt
     7441 gtggtccaac agtttataac tatatacaca taggtaatgc aagaccattt ataatatttg
     7501 atactctaag aaggtattta gagtatagag gatatgatgt aacttatgta caaaacttta
     7561 cagatgttga tgataagatt ataaatagaa gtcatgagga gggaatatct cctgaagaag
     7621 ttgcggctaa gtatataaaa gaatattttg tagactgtga tgggctagga ataaaaagag
     7681 ctactgttca tcctcaggtt acagataata tacaacagat tatagagttt ataaaagagt
     7741 tggaatataa gggatatgca tatgcagtaa atggtgatgt atattttgat acaaataagt
     7801 ttgaaggtta tggaaaactt tcaggtcaaa aacaagaaga tttagaggca ggggctagaa
     7861 ttgaggttaa tgaccaaaaa agacatccta tggactttgt tctttggaag gcaaaaaaag
     7921 aaggtgagcc aggatgggat agcccttggg gagaaggtag accaggatgg catatagaat
     7981 gttctgttat gtctaagaga tatttaggtg aaacaattga tatacatgca ggtgggcaag
     8041 atttaacatt cccacatcat gaaaatgaaa tagctcaaag tgaagctaga agtggaaaaa
     8101 tattctcaaa atactggatg cataatggat atataaatat aaatgatgaa aagatgagta
     8161 aatcaaaagg taatttcttt acagtaagag atatatcaaa attatatgat ttagagatag
     8221 taagattttt catgttatca gcacattata gaaatccagt aaactttagt gatgagatgt
     8281 taaatcaagc taaagcaggt cttgaaagac tatataatac taaagaaaaa ttagaattta
     8341 cgctaagtaa tttagtagaa tctccgttaa cagaaaaaga agtagagctt gttaaagaat
     8401 tagatgactt tagacaaaaa ttcatagatg ctatggatga tgatgttaat acagcagacg
     8461 cagtaagtgt aatatttgaa ttagctaagt taataaattc taatgtagat gaaaattctt
     8521 ctttggagtt tgctaaaaaa tgcttagatg aatttaatga acttacagga gtattaaata
     8581 ttgtaaataa gaaaaaggat actgtattag ataaagacat tgaagaattg atacaaaaaa
     8641 gaactgatgc aaagaaaaat aaagagtttc aattagctga tgatattaga caacaattac
     8701 tagatatggg aattgtatta gaagacacaa gacaaggtgt taagtggaaa aggatataaa
     8761 aatggaaaaa actgaattag ttactatgtc accattagtt ttagcatact taggtgatac
     8821 agtgtatgag acatatataa gagaatattt aattagacag aatacacaaa gaaaagttaa
     8881 tgatttgcat aaactagcta taaagtatgt aaaagctaaa gctcaggcaa ctataattca
     8941 tgaaattgaa attgagttaa ctgaagaaga gagtaaaatt tataaaaggg gaagaaatca
     9001 aaagtcaaat acttctccta agaatgcaga tattatagac tacaaacatg caactgggtt
     9061 tgaggcatta gtgggatatt tgtatttaaa taatgaaata gagagacttc aatatattat
     9121 caataaagga atcaaaatta ttgaaagaga tatgtaaata ttaaagagtg ttgtcttaat
     9181 aataagtaat aaggcaacac tttttttata actaatgtat aataaatata tagaaattag
     9241 taatgagaaa ggataaaaag ttatgaaagt aaaattaata tcacatacac ctgaaccaga
     9301 aaaagtaata gctatggcag ctaagctatg ttattcacca gttgggacag acgaaataga
     9361 aaaagactta acagatgaaa gtatagagaa atttttaaat atgttattaa gtataggtca
     9421 tgggtcaata ttagaacatg cttcatttac attttcaata gaaggtattt caagggcttg
     9481 ttcacatcaa attgttagac atcgtatagc tagtttttca caacaaagtc aaagatatgt
     9541 taaattagaa caatttgagt acataattcc tccagaaata gaaaaaatag aaaaagcaaa
     9601 agagttattt atagactcca tgaaaaaaga ccaagagaat tatgataagt tagttgaaat
     9661 attgtttgaa aatcattata atgacctaat aaaaaacggt aaaaatgaga aaacagctaa
     9721 aagacaagct gaaaagaaag caatagaaga tgctagatac gtatttccaa atgcatgtga
     9781 aacaaaaatg gtatttacta taaatgctag aagtttgttt aatttctttg aacatagatg
     9841 ctgtgaaaga gcacagtggg aaataagaaa tttggctgtg gagatgttaa aagaggttaa
     9901 aaaggttgct cctatattat ttaaaaaaac tggtccaagt tgtgtaaatg gaagttgtcc
     9961 agaaggaact atgacttgtg gagatatagt tcaagttaga gaaaaattta aggccttgta
    10021 ggaggtgaat aatttggcga gtatagaagg aagaaatccg gtaatagaag caataaaaag
    10081 cgacagagaa atagataaaa tattaattgc aaattcagct aaagaaggtt caattaagaa
    10141 aataatagga atggctaaag ataagaatat aattattcag tatgttgata aacataagct
    10201 agatgaagta agtacaagtc attcacatca aggtgtaata gcttatgcaa gtgagtataa
    10261 gtattatgaa ttagatgagc taatagattt agcaaagaat aaagatgaag acccattttt
    10321 cattatatta gatgagataa cagacccaca taatttgggt tcaataataa ggacagcaga
    10381 tgcagtagga gcacatggag ttatcattcc aaaaagaagg tctgtacaca taactcctgt
    10441 agttgccaaa gcatctgctg gagcagtaga atatatgcca gtttgcaagg taaccaatat
    10501 agttaataca ataaaaagac tcaaagaaga agggctgtgg attgctgcag ctgatatgga
    10561 tggagaaact ttttataaac aaaatcttac tggtcctctt ggtgtagtaa ttggaagtga
    10621 aggttttgga atatctagat tggttaaaca aaactgtgac tttatagtaa aaatgccaat
    10681 gataggtaat gttacatctt taaatgcatc tgtagcaggt ggaatacttt tatatgagat
    10741 attcagacaa agactggata aaagtaaata ggcatgaaaa aaaatataaa tcattactta
    10801 ataatagatg gctacaatat tataaatgcg tgggacaatt tgaaagaact ggctaaagag
    10861 gacttagaag attctcgaga aaagcttatt gatgacatta tagaattttc tgagtttatg
    10921 ggttataaga ctataatagt ttttgatgct tataatgtta aaaattctcg agaaaaagtt
    10981 gaaaaaagaa aacacataac aatagtttat acaagagaac accaaacagc agatagttat
    11041 atagagaaat ttataacttc tttatcaaag tatgatgatg taaaagtagc cactaatgac
    11101 tatgcagaac agcaaatgat tttaggaaaa ggtgctacta gaatgtctgc aagagaatta
    11161 aagttagagt tagatcgttc caagaataaa atgaaggaaa aaaatatcgg tttgaggaaa
    11221 aaaattcagc gaaattggtt ggaggaaagg ttagataaag aaacattgtc gaaacttgag
    11281 aacattcgta gaaagcgttg aaattactag gttctttaga ctataatata cctataaatt
    11341 tgttttatat atgggggaga tttacatgtt ggtagcaaaa gaaaaaagtt atgagttagt
    11401 ggacaattgt cagcaggatg agtataacat tgtattaaga gcaagtgaag gggataaaat
    11461 agcactagag tacattatta ctaaatacag aaattttgta aaggcaaaag caaagtcata
    11521 ttttttaatt ggagcagata aagaagatat aatacaagaa ggaatgatag gcctttataa
    11581 ggcagttaga gattttgatg gaagtaagac caattcattt aaatgttttg cggagatatg
    11641 tataacaaga caaataataa ctgctataaa aactgcaaca agacaaaaac atataccttt
    11701 aaattcatat gtttctttga ataaaccaat atatgatgaa gagtcagaca gaacactttt
    11761 agatattata gctacaagta tagtaacaga tccagaagag cttataatta gtaaagaaga
    11821 gttaaaaaat atagaatcaa aaatgaacga attgttaagt gacttggaat tagaagtact
    11881 agaattatat ttaaatggaa agtcatacca gtttatagcg gacaagctta aaagagatgt
    11941 aaagtcaata gataacgctt tacaaagggt taagagaaaa ttagaaaaac atcttgaaaa
    12001 cagaaatgat taatagtaaa atacttacca taagcttatt gtgagcaaaa ttaatttata
    12061 tcgaaagata agggaggaaa ttcaaaatgg ctaaagctaa atacgaaaga acaaaacctc
    12121 atgttaatat agggacaata ggacacgtag accacggtaa aactacatta acagcagcaa
    12181 taacaaaaac attatatgac agatatcaat taggagaagc agtagatttc gcaaacatag
    12241 ataaagctcc agaagaaaga gaaagaggaa tcacaatatc aacagcacac gttgagtatg
    12301 aaacaccaaa tagacactat gcacacgttg actgcccagg acatgctgac tacgttaaga
    12361 acatgataac aggagcagca caaatggacg gagcaatatt agtttgttca gcaacagatg
    12421 gaccaatgcc acaaacaaga gagcatatac tattatcaag acaagttgga gtaccatata
    12481 tagtagtatt cttaaacaaa tgtgacatgg tagatgatga agagttatta gagttagtag
    12541 agatggaagt aagagattta ttaacagaat atgatttccc aggagatgac actccaatag
    12601 taagaggttc agcattaatg gcattagaag atccaaagag tgagtgggga gataagatag
    12661 tagaattatt cgagcaaata gatgagtata taccagctcc agagagagat acagataaac
    12721 cattcttaat gccagtagag gatgtattct caatcacagg aagaggaaca gttgcaacag
    12781 gaagagtgga aagaggagta ctaaaagtac aagacgaagt agaattagta ggattaacag
    12841 aagcaccaag aaaagtagta gtaacaggag tagagatgtt cagaaaatta ttagaccaag
    12901 cacaagcagg ggataatata ggagcattat taagaggagt acaaagaaac gagatagaaa
    12961 gaggacaagt actagcaaag actggatcag taaaggcaca cacaaagttt acagcagaag
    13021 t
//
LOCUS       DAEMIK010000127        12967 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEMIK010000127 DAEMIK010000000
VERSION     DAEMIK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.128"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(21..1220)
                     /locus_tag="KPK87_002947"
     CDS             complement(21..1220)
                     /locus_tag="KPK87_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG2539072.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(1743..3080)
                     /gene="xylA"
                     /locus_tag="KPK87_002948"
     CDS             complement(1743..3080)
                     /gene="xylA"
                     /locus_tag="KPK87_002948"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG2539073.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLESILNRYIYEVDTISNK"
     gene            complement(3176..4726)
                     /gene="xylB"
                     /locus_tag="KPK87_002949"
     CDS             complement(3176..4726)
                     /gene="xylB"
                     /locus_tag="KPK87_002949"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG2539074.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            4934..6094
                     /locus_tag="KPK87_002950"
     CDS             4934..6094
                     /locus_tag="KPK87_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG2539075.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYKDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(6250..6654)
                     /locus_tag="KPK87_002951"
     CDS             complement(6250..6654)
                     /locus_tag="KPK87_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2539076.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(6675..7157)
                     /locus_tag="KPK87_002952"
     CDS             complement(6675..7157)
                     /locus_tag="KPK87_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2539077.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(7177..7986)
                     /locus_tag="KPK87_002953"
     CDS             complement(7177..7986)
                     /locus_tag="KPK87_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2539078.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(7979..8758)
                     /locus_tag="KPK87_002954"
     CDS             complement(7979..8758)
                     /locus_tag="KPK87_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2539079.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(8758..10836)
                     /locus_tag="KPK87_002955"
     CDS             complement(8758..10836)
                     /locus_tag="KPK87_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG2539080.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(11636..12157)
                     /locus_tag="KPK87_002956"
     CDS             complement(11636..12157)
                     /locus_tag="KPK87_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG2539081.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNNYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            12323..12889
                     /locus_tag="KPK87_002957"
     CDS             12323..12889
                     /locus_tag="KPK87_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2539082.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSYS"
BASE COUNT         4171 a         2293 c         1434 g         5069 t
ORIGIN      
        1 ttttaatttt attcttttat ctaataatta atattttcaa gttctagtgc ttttcttata
       61 caattatcat ctttaaaatc agcagtcctt ttacaatctt ttactttaaa ttctggaata
      121 gttttaaatt cctcatcaca tattccattc ttatttaaat acatacaact tgccattcta
      181 actattagac cactattttt taaattaaac aatacagggt ctatacaccc tccatctccg
      241 ccagtagttt caccaactaa agtcgcaaat ttattttctt tacaaaacat tgcaaatgac
      301 tctgatgatg aatatatttt tctatcaaca agtaagtata tattaccctt aaattttgaa
      361 tcatctgtag attttatagt ataggaagta tcttcaaaat cattaaaatc ttttataatt
      421 tctttaggtg cattttcctt tacacttgct ggtaaatttt ctacaggatt taattttata
      481 tttcttgcat ttgtatattt ttttactatt tcattattat ttctataaat tctataacca
      541 tttattttga catcattttt tatgagttta gatacgattc cttgccaata actatcactt
      601 ccacctaaat tacctcttat atcaataatt aaagctttat gtttttctaa agtgttaata
      661 taatctccaa ttatttttaa atcattattt atagagccat tctttgatga cattgatggt
      721 aaatacatat atccaatctg acccttaata acatccttta aaattaattc tttttttaca
      781 aaaatatcat ctggcatttt tattttaaga ttcatagagt catatctatc cacaactttt
      841 ttatcattaa gaaaatcata ccaatcatta ttagagtatg atttcttaaa aagttcatat
      901 cttttcttat tatctattac ttctgtgtgt ttattattta aatcagataa tatagatgac
      961 agttcttcta taaattcttc atcattttca gtgtttttta ttcgttttgt atattcatcc
     1021 ttatttgcta accaatcaat attattaagc ctcttattta cacctagata tggatatcct
     1081 gtctttacaa cattatacac ataattaaag tcatccacct tttcctcttc agacaactga
     1141 ttcttttgag aataagtaga attacctaat aatattataa atgctaaaat aattactaaa
     1201 aataatactt tctttttcat gtaattctca tccactttca gattttatat tttaatacat
     1261 tgtatcatta ttttattatc acctaaacag ttaaaagaaa ttcttgaaga tatattccac
     1321 tattattcta tttaaaaatt tttatttgta ttttcaaaat tatttcatga aagttataaa
     1381 ataacaatta tattcaaata tctattttac tgctatatcc tgaaagtaaa ttcttatcag
     1441 tgatatacta aaaaagatat gctaaaaaat ttatccatta taatgactta aatttagata
     1501 ctttactgat ttttatttat tagttgagaa agatattgat aattaaaaat attgataact
     1561 atagaaatga ttatttttta tagaaatttt aaggatataa actaattata cttaagtcta
     1621 tatccttaat ttatcatgat tatgagaatt tttacaaata cttatctttg tccgttttag
     1681 aataaaaaca agaacatatc ctacttattt tattcaaaca tccaagttta attactacac
     1741 aattacttat tagaaattgt atctacttca tatatatatc tgttaagtat agattctagc
     1801 atttcttgtc ttccagactt attctttata actggcattt gcagtgcata agattctaac
     1861 tctttaaatc caaccttacc ttctactata tccttaccta taccttcact aaaacttgca
     1921 tatctttctt tagtaaagtt ttcaaatacc tcatcttcta ataatttatg agctattaat
     1981 aatccctttg caaatgtatc cattcctgca atataagcta ggaataagtc ttctacctca
     2041 aaagatgctc ttcttacttt agagtcaaag ttaaaccctc ctggtgctat tccaccttgc
     2101 tttaacactt catacatagc aagtgttgca tcatatatat ttgttggaaa ttggtctgta
     2161 tcccaaccta ataataggtc tccttgattt gcatctatac tacctaaaac attgtttatt
     2221 cttgctatat ttaattcatg ttggaaagta tgtcctgcaa gtgttgcatg gtttgcttct
     2281 atattcattt tgaagtattt atccagatta tactttctta aaaatcctaa aacagtagca
     2341 gtatcaaaat cgtattgatg tttagtaggc tcttttggct taggctctat taagaattgt
     2401 ccagtgaatc ctatttcctt agcataatct acagccattt gtaagaatct tgcaaagtta
     2461 tccatttcaa gttctgtatt agtgttaagt aaagtttcat aaccttctct tccaccccag
     2521 aatacaaaat tttctcctcc taagtactta gttatttcca tagctttttt aacctgagct
     2581 gctgcatatg catatacatc tgcattacat gttgtagatg caccatgaac aaatcttgga
     2641 tttccaaaca tattggcagt tccccacaat aactttatac ctgtcttttc catattaact
     2701 tttatatact caactatttc atctaatatc ttatttgtct cttctaaatc tcttccttct
     2761 ggtgctatat ctctgtcatg aaaacagaaa tactcaattc ccatcttaga cataaattca
     2821 aatcctgcat caactttaat ctttgctatc tcaattgggt ctgttatatt attccaaggt
     2881 ctctctactg tccctactcc aaatgggtca ttaccctctc cacataaagt atgccaatag
     2941 ctcattgcaa atcttaagtg ttctttcata gtcttattac ctaccacttg ttcaggattg
     3001 tagtatctaa atgctaattc attgtcagat tttactcctt caaattttat ctgtcctatt
     3061 cctttaaata tttcactcat ttcatgtcct cctaatattt tacacatata atatatgtag
     3121 tatgttttct taatttaaaa ctattttcta tctttatttt tctttttaag ctaatttaat
     3181 tatttatttt tgcattaact acttcatcta ataacttaaa gtcatctttc aaagatttgt
     3241 atattctttt atacaatcta tgatatatat tgtatgtatc tacattttca cttattggtt
     3301 ctaatttagt tgaagttttt attatagcat cacatgcttt ttgtatattt tcatatattc
     3361 cagctcctac taatgcaaga attgcaactc caagtgctgg tccttcactt gaatttatag
     3421 tatatacatc ttgattaaac atatctgctt gcatctgtct ccatacttta ctttttccac
     3481 ctccacctga ggctcttact tcattaacgt ctatattaag attctctatt atatccatac
     3541 agtctttaag actatatgaa acaccttcca ttattgacct tgcaatttct tttttagaat
     3601 ggattggaga taatccaaag aacacacctc ttgcataagg gtcaagatga ggagttcttt
     3661 ctcccatcat atatggtaag taaagcaatc cattacatcc tgttggtact tgacttactt
     3721 gattatttat aatctcatat acatcttcac ctaaagaatt tgaaacttcc acttcacttt
     3781 gacagaaatt atctttaaac cattttaatg acaatccagc tccttgagta actcccataa
     3841 catgccatgt atttggtatt gcatgacaga aagtatgaac tctaccttct ttatcaattt
     3901 ttggctcttt tgtataagca aatactacac ctgatgtacc tatagtagat gaaactactc
     3961 catctttaac tattccattt cctatagctc ctgctgcttg gtctccagct cctcctacca
     4021 ctattgttcc ttctttaagc cctgtcaaac ttgctacatc tctagtcaca taacctgtaa
     4081 tctcttgtga ttcataaact ttacctaaaa gatttttatc tatatttaac ttttctagta
     4141 cttcatcgct ccaatctctt tttcttatat cgagcaactg cataccactt gcatctgata
     4201 cctctgtagc atattcacct gtcaatttgt atcttatata atcttttgga agaagtattt
     4261 tattagtatt tttatatata tctggttcat tgtttctaac ccataatatc tttgatgctg
     4321 taaatcctgt aagtgctgga ttagcagtta tttcaatcag tctttcttca cctactaaat
     4381 cagttatctc ctcacactct tttactgttc gttggtcaca ccatattata gagtttctca
     4441 atgttttacc ttcattgtcc agcataacta gaccatgcat ctgccctgat aatccaattc
     4501 cttttataca ttcactgctt atatttgaac tttgaactac ttctcttata gattcgacta
     4561 cagctttcca ccaatcattt gcatcctgct ctgcccaacc tgatttctct tgtataagag
     4621 gatattcata tgtacaactt tttatagtat ttcctagttt atcaaataaa actgttttag
     4681 tacctgatgt accaatatcc acacctaata catactccaa cttcatccct ccttgaatgc
     4741 cacttcataa ctatcgtttt cctatcctaa tttttttata ttttaggtat attttacttg
     4801 ctttattttt gttagtatat taaactaact tatgaactaa taataaaata tttaattatc
     4861 tttgtcaatg caaaataacc ttttattttt gtgtaaaaag tattacaata ttattttaag
     4921 gaggaatatc aaattggtta ctgataaata cactattcga gaaatgaatg aaagattggt
     4981 acttgaacaa attataaaaa atggtcctat ttctagggca agtatagctt ctactatagg
     5041 attgaataaa gcaactatat ccgctattac caaaaaactt attgatgaat ctctagttca
     5101 tgaaatcgga attggaaata gtactcatag tggcggaaga aaacccatat tattagtatt
     5161 taataaatgt gcagggattt ctttaagtat ggacattgga tacgactata tattttcttc
     5221 tctatcttac ttagatggaa ctattataaa ttctaagaaa ttaacagata tacaagtaag
     5281 taaagacaat gttatacaat tgatagatga aattataaat agttataaca tatccaaaat
     5341 agacactcct tataaggtaa ttggtcttac tttggctata catgggatta cttgtgaaaa
     5401 taaagtattg tttactccat attacaatct taatgagatt gacttatata gcatccttag
     5461 caaaaagtat gactttccta ttcatataga aaatgaagca aacttaacag ctttagctga
     5521 gaatactttt tcaactgtac ataactcact acttagtctc agtattcaca gtggatttgg
     5581 ttcaggcatt ataataaata ataaattata tagtggcaga aatggtatga gtggagaaat
     5641 tggtcacact atcatcatgc ctaatggtaa actatgtcct tgtggaaatc gtggctgttt
     5701 agaacagtat tgttcagaaa aaaaggtatt tgaacagctt tcttctttag aaaatattcc
     5761 taaaatagat tctgatatag taaagcaact atattataaa gataaccaga atgccaaaaa
     5821 agttattcat gagttttgta gttacttaac aatagctata aataatgcta ttacaactta
     5881 tgctcctgaa ataatatatt taaatagcca aataattagt gatatccctg agatattaca
     5941 gattacaaaa gatatgttag ttagctcttt taacaaagga ataaatatag aaatttcatc
     6001 attagggtct gaggcatctc tatatggtgg ctctgccgta aatatcaaga gctttttgaa
     6061 tatacaaaat ttaactttaa ttaatgaaat ataagggtta tgactatata cagccataac
     6121 cctttaaatt atacattcta aaattagatt tattatatag tttatgcaag attagctata
     6181 ttttttaaaa attgtatatc aattaagtat taacataagt atttcgtttt aaatattaat
     6241 ataatcttcc tactcatcat cagataataa acttgcattt tgttcaacca tagaaatatt
     6301 atctttacct attttctcta attttgctgc caattccttt gtatctaaaa aagctgattc
     6361 aataaccata cctaaattta gccctgaaat aattttaatt cgactgtcat tattggctac
     6421 taataaagca gcattgcatg gagttccacc tagtaaatct gccattataa ttatttctga
     6481 actttccttg tacacttcta aagctttttt taatttttct attgttcctt ctattccatc
     6541 atcttgtttc atatttacag taggataatc tatagaatcc cctattatca tttttgcaga
     6601 atttaagact tcttcagcca tattgccatg actcatcaaa ataatactat tcactttaat
     6661 acacctcttt aaatctataa gatgtctaca agataagttt tcttatcatc aggcgtcatt
     6721 atagttgtaa tttcaattcc ttcatctaaa agtcttttaa atgcttcttt ctcttcttca
     6781 gtaacactga tattaggtct tagaactgtt gtatcttttc tacttgacat atttcctaca
     6841 tttatattat gtattttaaa tcctgcctct acaaatggaa taaggtccac aggagactta
     6901 acaattaata atacattttg tccttcatac tttcctgcag taatattttt tatagcagtc
     6961 tcttctgtaa ttatagatga cgctatacct gcaggtgtag ccattcttaa aacttgtttt
     7021 tgaacagaat tgttagctac tgcatcattt actaccatta atcttgtaac acccaattta
     7081 ttactccaca tagttgcaac ttgaccatgg attaaacggt catctatacg aatattttta
     7141 atacctttca tttccatatc taatatcatc ctttccttat tttaaaatac ctaatgcata
     7201 taaaattatt gaaacaacca ttactcctaa aattaagtat ataggtttta tattcttttt
     7261 aagagcccaa taaacaatca caactaaaat tgcaggaact aacccaggca taatttggtc
     7321 taatatttct tgacctttca ttgtaaaatc accttgttga aagttcaaag caacattagc
     7381 ttttacaact gaaggtatta atgcacctac aaccattaaa cctaaaatag atgtactctc
     7441 agttaaaatc tttaaacttt tttcaattga agatattagc ttagtacctg atgtataacc
     7501 agcattaaat agtggtatac ttaataattt caatgctaca ctcactacaa tccacagtaa
     7561 gattccaaat ggacttccat ctatcgccat attagctgct atagctccaa aaattgtcca
     7621 cggaataacg acaaataaac tatctcctaa acctgctact ggtcccatca agcctgtttt
     7681 aattgaagca acagcctctt cagtctcttc acttgataac tcctccattg ctacattaat
     7741 acccaaaata taaggggcaa cccaaggatt agtgttaaaa aattgtaaat gatttaatac
     7801 agcactttgt aacttttctg gattatcttt gtatactact tttaaaaaag gtagtactgc
     7861 ataacagtat gccaaggcct gcatcttctc ataatttgaa gaaacagaac tagtaaaaaa
     7921 ccagcgccat aagacacttt ttttcacctt ttcatttgat ttatttaatg ataccttatc
     7981 actcatcttc gtccatacct cctactacag tatattgttg ttcttgattt tccaaattct
     8041 ttttgtaaat tataagtgca attgctccac caattaaagc tacacctaat aatggtactt
     8101 tcatatatac tgctaaaaca aaaccaatta ctaagtaact aaaatactcc ttagcaggaa
     8161 gatatctaag taataatcct atccctactg ctggaagtag tcctccagct actgttaaac
     8221 cacctgttaa ccattcaggt gtatatttca aaatggtatt aattaaagaa ggtccaaata
     8281 ttacagatag agcaactgga atacctgttg tagctgctgt taatagtact cctaacattt
     8341 gcatataggt tatattttta taatttcctt ttttagctcc atcttctgct ttatgaacta
     8401 gccatatacc aatagtattt ctaagaacat ctaattgtac catcaataaa gcaactggaa
     8461 tacctataga gattcctaca gatgcttctt gcttagtcgt aatagttata aatgtggcta
     8521 ctatacttgc tgtttgataa tcaggtattg atgctccacc aaagttacta atacctaatg
     8581 ataatagctg taatgtacct ccaatatata accctgtatt tatatctcct aagataagac
     8641 cagtaataaa acctgctgtt actggttgat atgttccaaa cattgttgaa tttttttcat
     8701 agtttataaa aaatccatat agtacgatta ataataattg ccataattgt ggctccatta
     8761 tgatttcacc tctcccaaaa tattttttag ttctttaaaa ttataactgg aatcttgctt
     8821 taggaaaata ggaatttctt ccagtgggca ttcaacttca taagttttcc caccttcata
     8881 actaactcca ctaaatatat tttcccatgt atgtcctttt ggtaaatata cttttctact
     8941 tctatcttta taattcatta ttggagctac taaaatttca tctccaagca tatatgtatt
     9001 atccacctcc caagttttat catcttcagg aaattcataa aataaagttc tcataactgg
     9061 tgttccatct tcatgcgctt ctttcatcag tttttttaaa taagttttta agctctctct
     9121 aatttttatg aattttgtaa gaattacttc aacttcttca ccataagacc atatttcatt
     9181 aggagctcca gttaccattg agcctccacc tttatttgat aatggtttac tatgaggcaa
     9241 tctatctcca tgcattctca aaattggtga aaaagtagca tattggaacc atcttaccat
     9301 aagttctcta aattctgggt ctttaggatt tcctccatgg aaacctccaa tatcagtagt
     9361 ccaccatggt atacctgcca gtcccatatt taaccctgta tttacttgat tattaaaagc
     9421 ttcaaaacta gaatcaatgt caccagacca tactaaagct ccatactttt gagctcctgc
     9481 ccaagctcct cttacaagag ttacaacaga ctctatgtca ttatgtaaac catcataaat
     9541 catttttaaa taatcaatag gataaatatt accacaactt aaaacatttc cttttttata
     9601 tctatagtta tcaaaatcat acacagaata gccaggttca gctacatcta accaataata
     9661 gtcaatccca agttgtttat aatttttatc tattctatcc caaacatatt ctcttgcatt
     9721 ttcatttgtc atatctacaa agactgtatt tccttgaatt tgcattgtca ttctaacacc
     9781 acgattaaca ttgactaaat agccattttc taaataatca ttgtagtttt ctgcatcact
     9841 ttgaactgta ggccatacag atactattgg ttctactgaa tactcttctt tcaatttttt
     9901 aactaattct ttaggatttt tccaataatc taagtcaaat ttatattcac cttgcttagg
     9961 ccaatgaaaa tagtcaattg caattgatga aagcttaatt cctcttttag aatattcctt
    10021 cacaacatct aaaacttctt ctgaagttct atatcttagt ttactttgcc atagtcctaa
    10081 aaggttttca ggcatcatag gaacagttcc agtaacttga gagtaatttt tatttaactc
    10141 ttttgggctt tctccacaag taatccaata atctataaaa tttgtagaaa acatcttcca
    10201 ttctgtcata tttttagcaa aagatacttt tccaattccc ggattattcc ataaaagtcc
    10261 ataacctaga ctagaaatat aaaaaggaac tgatatttga gaatttcttt gggctaactc
    10321 caaaacagta ttctttaaat ctaaaaattt atgttgatat tgtcccattc caaaaatctt
    10381 ttcagaaggc tcagattcaa atctagtagt tacttcaaat tctccactat agcttggtcg
    10441 atattctctt gattttaact ttagagtaga gttaaagtct ttggtaatct caattgtccc
    10501 tacatcttct ccaccatcat catgttttac agctcttaat cgaatatatt ctttcaataa
    10561 tatttcattt ttatcattat aaaaagtaat tctatctcta tggtctaaga cagcttttat
    10621 ctttccattt ttaatgattg cacttccatc ttcattttta ttaattgtaa tatcatcttt
    10681 atttaactta ggtttttcgt taagagcata gttttcatca acaaaatttt ggtctacaaa
    10741 cgacctaata cgtaaagagt tttttcccca tgcttctatt cgcaatagtt cattgtcaaa
    10801 tcttttttca atatagtctt cagatatttt tatcacggct acctcctaaa tcaatggtat
    10861 aatattctat ctttgtgtag cttaagttaa ataatgtcgt ttagtatcgt tatttataaa
    10921 ttaggtttta tatataattc atttgtacat taaactaact taccaataat atactttatt
    10981 tgactttgtt tgtcaatcgc tttccggatt ttagtaaaat tttctaatat gttgcttgaa
    11041 agtcgttctt aatatctttt atttatactt ttaatatctg tgatgatacc tatatacatc
    11101 tagttttttt aaaacacata caggaataac gtttgcctat tatgtagata atatcggact
    11161 tgaaaatatg tatagtgacc ttataaatac agataataat atattagata ttgttcaaaa
    11221 caatggattt tataattata aataatttat gaaattattt aataaaatat ataattgtat
    11281 accaagtcaa aaacgacaac aaataaataa aactaaaaaa gatgttgtct caaaatatag
    11341 ataaatttat ttttcattgc taaaatgatt aaaatatatt tgatttaata tacttgattt
    11401 aatataaaaa aaagagtgtc tcataaacta aaaagttcac ttttagacac tcctttatat
    11461 atattatcta cttcttaatt catttattac ctctctacat tttctttcaa ctagtggata
    11521 catctttata agtactaatt actccactta aaaatgattc aggtactgtc catcagtcat
    11581 atttttctgc caaatataat ggtgtttcat aatgagacaa taaatattta tatctctact
    11641 tatcttctct aagctcgtca aatccaacta taggtatatt taaattatat tctttaagta
    11701 gcttttctag ctcttctata aaaatacctt tcccatccac catttgtata tcatttattt
    11761 tatccatcaa ttcattgcaa ccacttaact caccaaacca acgtttagaa ctacatgtct
    11821 tattataacc acaacttgga ctgccatcta ttgcaatgat actttttata tcataattat
    11881 tttttaaata ttcatcaaac tgccttacat gaggtattaa catttgccta cattgttctc
    11941 taaaaaacaa attgtcaaac tgttctttta catgtcccca cctatttatt ccatacataa
    12001 tcatttctgg gcatggtagt tgaattattc catacccttt atttattaaa tctatcgcta
    12061 tattttgtgc tcccttaaat tctgacaatc cctctacttt agaattacaa tttaatatac
    12121 aatgacatac tactacaatc tctctatttc tcttcataat aatctcctta gttattctta
    12181 attttaatga tactacatat tttgataatt atattattta tttgttctat atttccaata
    12241 ttctatatag aaatatacaa ttagtaatct tttataatct atactatgat aaatgtatat
    12301 atttttttag gaggtattat aattggatac taaaaaagtc acattttctt caatgtgcat
    12361 agttattaat attgtcttag gtatggttat atctatgata aatataccat tattattttt
    12421 agatacagtt ggtactgtac ttggggctgt cgtattaggt cctttttggg gtgctcttat
    12481 tggtgggtgt acaaacttag ttttaggtgt tatatcaggt cctacaaata taccttttgc
    12541 ccttgtaaat attgttcttg gtcttattgt tggatatatt tctaagaaaa aaggttttgg
    12601 ttataaagaa gctattatta caggagtaat tttatctatt gtttgtcctt taattggcac
    12661 tcctatttca gttttattgt ttggtggttt aagtggaagt ggcgctgatt tattggttgg
    12721 ttttttagtt cagtctggtc agaagatatt tactgctgcg ttctttccaa ggattcttag
    12781 taatatagta gacaaacctt tgtcttgtat tatggtggta tattttataa gtaaaatgcc
    12841 taaaggtttt ataaaacaat atagtaaaaa tacttctagt tattcttagt aaatttgtac
    12901 atactatatg attttttaaa tgttataaca agtttataca aatatatttg tataatatct
    12961 ttgctac
//
LOCUS       DAEMIK010000128        12867 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEMIK010000128 DAEMIK010000000
VERSION     DAEMIK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.129"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(499..687)
                     /locus_tag="KPK87_002958"
     CDS             complement(499..687)
                     /locus_tag="KPK87_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539083.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            complement(713..1852)
                     /locus_tag="KPK87_002959"
     CDS             complement(713..1852)
                     /locus_tag="KPK87_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2539084.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKIIEDKNDLALESYYSSVIEGAFSNRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            2437..3348
                     /locus_tag="KPK87_002960"
     CDS             2437..3348
                     /locus_tag="KPK87_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539085.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRQQGRDEKVKMTAIEYTIYEQPQPPAL
                     DCPVLENPTADNPILENPTPDNPTSENPTQLNKDIQKTDLSKKEKSNTDLSSNHSIPI
                     LSPNPSPLREETAEPERKGTEAADAYSVYEEIIKDNIEYEHFIKHTDIDRERLDEIVS
                     LILETVCTKRKTIRIAGDDYPAELVKAKFMKLNSSHIEFVFDCMKENTTKIRNIKQYL
                     KAVLFNAPNTIDSYYTALVNHDMYGGY"
     gene            3368..3586
                     /locus_tag="KPK87_002961"
     CDS             3368..3586
                     /locus_tag="KPK87_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBG2539086.1"
                     /translation="MTQKTGALIFDETADRYDIRFDLNDYYGGLHCGDCFEVFVRGKW
                     KPTRMEYGDNWYLVGVRASDLNGLRVRI"
     gene            3669..4145
                     /locus_tag="KPK87_002962"
     CDS             3669..4145
                     /locus_tag="KPK87_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG2539087.1"
                     /translation="MQDEVNEKVVSLAIKTSKLTAEVLQKAMKALLAKGKGQLTKAPH
                     GKITMRQLMKPGEKVSNIEITDQNIKAFDPIARKNGLDYNVKKIENGKPPTYLVSFRG
                     KDIDVMTEAFREFTAKKLGRDKKPSIRKLLSTLKDKAAALNAQRDKVKKKDREVSL"
     gene            4142..4666
                     /locus_tag="KPK87_002963"
                     /pseudo
     CDS             4142..4666
                     /locus_tag="KPK87_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529212.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            4719..6362
                     /locus_tag="KPK87_002964"
     CDS             4719..6362
                     /locus_tag="KPK87_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2539088.1"
                     /translation="MRTATQTMKFTALYERLSRDDELIGESNSIKNQKQLLESYAHKN
                     GYSPIRHFTDDGVSGTTFEREGFQAMIAEVEAGNVSAVIVKDMSRFGRDYLKVGFYTE
                     VMFKEKGVRFIAINNGIDSANQQDSDFTPFLNIMNEWYARDASRKIQAVFKSRMQDGK
                     RVSPSVPYGYLRSPEDKQKLIIDEEPAAVVRRIYQMVIEGKGITAIADILTAEKVLIP
                     SAYAKIHCPENDHSKGFTNPYLWSATAVSYILEKQEYMGHTVLGKTISVSYKTKKRRK
                     AEPDELMIFKNTHPAIVDEETWHLAQKLRKTVRKPSYNRPPHPLTGLVYCADCSSKMT
                     HRQPSPTKKKKYDADDAYICGSYRQRTKDCTMHFIKTSVLWELILTAIREVSDYVRQD
                     EQAFIDKVQQTSIVQMAETQREQKRRLAEATERNEELNTLIKKLYEGNATGKIPDKHF
                     ERLLAEYDREQTALETEIEDLQEQIDSFDEDNTKVDKFIAVVRRYTDFTELTTPMLNE
                     FIEKVVVHEATGGRTDRKQKIDVYFNFVGQVELPPPDRA"
     gene            6420..6698
                     /locus_tag="KPK87_002965"
     CDS             6420..6698
                     /locus_tag="KPK87_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539089.1"
                     /translation="MRLSRYEKETIILLNAAEDTASIYTADPVWKRKLDKLVEKNPQC
                     YQCVKADEVSKTYSMPKRFISLRSKEKTVTLTDEQKKRAKQRLTRTDK"
     gene            6843..7220
                     /locus_tag="KPK87_002966"
     CDS             6843..7220
                     /locus_tag="KPK87_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBG2539090.1"
                     /translation="MSFPYFYGRAAEQYAFYRIPKLLFSDERFEALSTDAKLLYGMLI
                     DRMELSMKNGWTDKDGRVFIYFTLEDARERLKCGTEKIVRLFAELDSEKGIGLIECVR
                     QGLGKPNIIYVKNFMDDTSLYDW"
     gene            7475..7828
                     /locus_tag="KPK87_002967"
     CDS             7475..7828
                     /locus_tag="KPK87_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG2539091.1"
                     /translation="MKKMNYLGLLSLLSLIAILGWKTGNTGLYGFFGFAYYIRYFWVI
                     PDELFQQNVRKSTTFAFFAEMISLVPFMFICATLYDSSSAIPMAFGLSFATAILTFTV
                     VLMIQEWKERKGAQE"
     gene            7825..8019
                     /locus_tag="KPK87_002968"
     CDS             7825..8019
                     /locus_tag="KPK87_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539092.1"
                     /translation="MIKNRIKEYRAKHDMKQEDLAKLVGVRRETIGNLEKGRYNPSLV
                     LAWNIAKVFNVTIEEIFTVE"
     gene            8152..8571
                     /locus_tag="KPK87_002969"
     CDS             8152..8571
                     /locus_tag="KPK87_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2539093.1"
                     /translation="MNENIEILQRQFDAYCTKVLKYASISYYHANRRRMEHEVSFSSL
                     LEKDFAEVSTTDRYPCMLYRFQVREWLVEVQSEPLGHAIERLTPYRQEIILLYFFLGF
                     KNKEIAALYGCNAGKISYHKMRAVRQIRAEMEVRRHD"
     gene            8564..8806
                     /locus_tag="KPK87_002970"
     CDS             8564..8806
                     /locus_tag="KPK87_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539094.1"
                     /translation="MTKKYKDRYVPVEVIIAATQGEAAAIQTVLKTYESYIRNKCIKT
                     VYDERGLVYYGIDTDKKDFMERRLIHAVVENFKIDR"
     gene            8934..9353
                     /locus_tag="KPK87_002971"
     CDS             8934..9353
                     /locus_tag="KPK87_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HBG2539095.1"
                     /translation="MATSNIKASIPCDIHSVWKVVTEVKNYTWRSDLSKTEVISDKQF
                     IEYTKEGYPTTFTITITEPYKRWEFDMENSNMKGHWVGIFTSKDTETEIDFTEYVTAK
                     KLFMKPFVKSYLKKQQAQFVTDLKKAILCLMEEQNGI"
     gene            9343..9738
                     /locus_tag="KPK87_002972"
     CDS             9343..9738
                     /locus_tag="KPK87_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539096.1"
                     /translation="MEYKVNDIKLVASTFIPFVNQIWKGNYDIERTQAALSKTLNITA
                     YDNKILVGCLRILTDGYFFGTITELLVLPEYQKQGVGSKLLELAKENTPTMLYFGAQP
                     GVEAFYEKNGCQKSLQSYIIEKPKEHITE"
     gene            9801..10103
                     /locus_tag="KPK87_002973"
     CDS             9801..10103
                     /locus_tag="KPK87_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HBG2539097.1"
                     /translation="MPEIRKEILTMTDIEEKIAKAEEEIRQLQNRKRKLLNQKKDAER
                     KARTHRLIERGAILESLLEKPEQYSNEQIKGLLEIAFQTAQAQEHLRKIKEENGEN"
     gene            10322..11815
                     /locus_tag="KPK87_002974"
     CDS             10322..11815
                     /locus_tag="KPK87_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBG2539098.1"
                     /translation="MAIYHCSIKIVSRGKGKSAVAAAAYRSGETLTNNYDGVTHDFTR
                     KGGIVHTEILLPPHAPPDFADRSTLWNGVEKIEKAKNSQLAREIEIALPVELDREQQI
                     QLVREYVKENFVSVGMCADFAIHDKQDGNPHAHIMLTMRPLEQSGEWGAKSKKEYLLD
                     KDGQRIKLKNGTFKSRKVDTVDWNSQEKAEVWRQAWADIANRYLAAQDRPERIDHRSY
                     KRQGIEQIPTVHMGVAATQMERRGIVTDKGNQNREIREQNRLLKEVKRRIAALTAWIK
                     EKTAQTKENPFPSVPILSERSEQPPTLLELLNRAYTEAAKPQSRYGKIQDLKLYAKAF
                     NFLQSRNITTLVQLQEVVSDMKKQYWTTNGEIKQTEKLLHERKELIDQAEKYLEHHPT
                     YKAYMQTKPKKQEDFFESNRAALILYQSAERYLKEHLGEDKVLKPKAWKAEVADLTQK
                     KSRLYSDLQTLKAEVQEAEAVKKCVEQAVQPEQTKEKTQNKRRDMEL"
     gene            11876..12349
                     /locus_tag="KPK87_002975"
     CDS             11876..12349
                     /locus_tag="KPK87_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539099.1"
                     /translation="MEYKIRQMKNTEYPLLNDFLYEAIYIPNGIEPPPKTVIYSPELQ
                     VYVSDFGQMKHDIALVAEIDKKIIGAVWVRIMNDYGHIDNTTPSLAMSVFKEYRGLGV
                     GTALLEELIATLKSKGYSQVSLSVQKDNYAVKMYQKVGFTILKEVDEEYIMVIAL"
     gene            12571..12750
                     /locus_tag="KPK87_002976"
     CDS             12571..12750
                     /locus_tag="KPK87_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HBG2539100.1"
                     /translation="MQENTQTATKTTAAPKPQTVKRKIGKTTYELSLHFSQTSTERMS
                     DKVMRLLENDLSVKK"
BASE COUNT         4181 a         2582 c         2742 g         3362 t
ORIGIN      
        1 aagtgctact ctttttacaa ataaatttta ttatttatac ttgtaattat atattaaata
       61 accaatttta tccatatgaa aaacaaataa ttacgatatt tttttaatta ttctttatat
      121 ttacatttta attcaaatta cttttgtaaa gattatatct gttgcatata ctactttaag
      181 tgctaatata tctcccccta aattagaatc ctcgttatct caatagacgt tttctactgt
      241 caaagttacc aacatttgaa acttattaaa tttttatata aaaaagacct agaatataaa
      301 ttccaagtca ttttttatta aatttatttt ttacaatata atattgtcat gattaaagaa
      361 cacaaatcat acacaaattg gtcataaatc ttacataaat catctacaaa aaatacatta
      421 tttatattaa atctatataa ctaaattaag agcatattgt acaatatgct cttagaaaag
      481 atatttcttg tttatagttt atagtttata gtatttctct atgattttta tttttatcat
      541 ttcaaaaata aaaattaaga ttacagttat tatcatattt tgtgtactat tttctataat
      601 attataatga aacaatgaaa aaacaataat taatgaaatt acgtttatag ccccagaaat
      661 caaaaatgcc tttttcttat tattcatata ttccctctcc agattatcta aactagtcta
      721 tatcaagcat ttttaagtac ttatcattaa ctttatatat atttttttct ttagacaaaa
      781 tctctatctc ttcaagatta ttaaacactt ccatcaactg gtcaactaat tcaatttcct
      841 ttattccagc ctttaagtat ctacttttat tcttttttat ataattttct aatgtcatag
      901 aatagttatc tttactacag atatatttta atatatgttc ttctgttgct gatagagtta
      961 tgtcattttc aaacaattcc tcttttaata tactttctaa agattttcta tcatgcatat
     1021 tacgcatctc attaatagat tgtattatag acttgagcat aaactctata aatacagtta
     1081 aattatttcc tttgttttct acatcaagta tagccttata atacttagtt ctgtttttag
     1141 atatcatata tgatattgaa aactccttga aagtatcata tcctttatca atcaaatata
     1201 gatatgataa ggctcttgat gttcttccat taccatcact aaaaggatga acatatacaa
     1261 agtaaaaatg aattatagca cttttaatta aattacttac tttgctattt tccataaaat
     1321 ctattaattt actcataaag tcatatatct tcaaaggttc aagccctata tgtataatct
     1381 caccttttga atcacataca tatacttttt cagttctata ctcatattct tctgaatcca
     1441 agcagttttc gcccaatata tgatgcaaat catatataaa tttgtgacta tataatttat
     1501 ctaagttgtc taatccatat tctaaggctc tatgattatt gtaaatcata tattcacttt
     1561 tattacttgg tttcatcttt cctctaatta tagattttgc aatctttcta tttgaaaaag
     1621 caccttctat tacactagaa taataagact ctagtgccaa atcattttta tcttctatta
     1681 ttttaaatat cttacttgat gttaatttat ctatttcatt tattaaattt aatatatttt
     1741 cagtttcatg atagtatagt tctttattat caaaactatt tatagaaagt acgattttat
     1801 ttccagtaaa gtatttgttt ttgtattctt ctattgtcag tttaatcacc accttagatt
     1861 atctttatct attaatatta acaaaaagta ttaattttta cactattttt gattatttgt
     1921 tttataatta tcaaactcaa tttaacgtta aagcataata aattttaata aataatgcaa
     1981 aaatttaaga ataagcattt aaaatatatg tcacaggaag tttacaacta ttatttcttt
     2041 taaattagtt gattatcttg aaaaatgctt gatttacggg catattcgca ggtttgcaga
     2101 ttgattttac tacttattta ctacttttaa cggattgcgc cttgatatgc aaaagcacct
     2161 gcggagctgc cggataaacc gctacaccac cttatgcggc acgtcgaaaa gaaaaaataa
     2221 aataagcacc acctataagg cggcgtttct cactcctaca caggagaaca ggaacgccgc
     2281 tatttttatg ccctcatgtt acgcagtaag gggcaaaaaa gccttgattt atgcggctct
     2341 taggacgtga aaatgagaaa ggcaagggtt gatacctttt ccctcaaaac cgcgtttcta
     2401 ttgcgtaaca aatccaacca aaggagtgat gaagctatgg cagttttccg cgtggaaaag
     2461 aataaaggtt atacggttat gagcaaccac catttacgaa acaaggagct ttccctaaag
     2521 gcaaaagggc tgttatcaca aatgctgtcc ttgccggagg attgggacta tacccttgca
     2581 gggctgtccc ttatcaaccg ggaaagtatc gacgctatcc gcaccgctgt atgggagctt
     2641 gaaaaagccg gatatatcac aaggcagcag ggacgcgacg agaaagttaa aatgaccgct
     2701 atcgagtaca ccatttacga acagccacag cccccggcat tggattgtcc ggtattggaa
     2761 aatccaacag cggataaccc gatattggaa aatccgacac cggataaccc gacgtcggaa
     2821 aatccaacgc aattaaataa agatatacaa aagactgact tatcaaaaaa agaaaaatca
     2881 aatacggatt tatcaagtaa ccattccatt cctatccttt cccctaaccc ctctcctttg
     2941 agggaggaaa cggcagagcc ggaacggaaa ggaacggaag cagcagacgc atacagtgtg
     3001 tatgaggaaa tcatcaagga caatatcgag tatgagcatt ttatcaagca tactgacatt
     3061 gacagggaac gcttggacga gattgtgtcc cttatccttg aaactgtctg caccaaacga
     3121 aagacaatcc gtatcgccgg ggacgattat ccggcagagc ttgtcaaagc aaagtttatg
     3181 aagctcaaca gcagccacat tgaatttgtc tttgactgca tgaaagagaa caccacgaaa
     3241 atccgcaaca tcaagcaata tctgaaagcg gtacttttca acgcacccaa caccattgac
     3301 agctattaca ccgcccttgt caatcacgat atgtacggcg gctattaaaa aacaggagga
     3361 ttttaatatg acacagaaaa caggagcttt gatttttgat gaaacggcag accgttacga
     3421 cattcgcttt gaccttaacg actactacgg aggattgcat tgtggggatt gctttgaagt
     3481 gtttgtacgc ggcaaatgga agccgacccg tatggagtac ggggacaact ggtatcttgt
     3541 gggtgtgagg gcttcggatt tgaacgggct gcgggtgcgt atctaaaggc ggcatgaaaa
     3601 atgtacgcct tatttttatg ccccgaagcg gcacaccacc ctaacaaaac catgaaagga
     3661 gggatttcat gcaagatgaa gtcaacgaaa aagttgtgtc tttagcaatc aaaacatcta
     3721 agctgaccgc cgaagtgctg caaaaggcta tgaaagccct gcttgccaaa ggcaaagggc
     3781 agctaaccaa agccccgcat gggaaaatca ctatgcggca gcttatgaag ccgggagaaa
     3841 aggtttccaa cattgagatt accgaccaaa atatcaaagc ctttgaccct atcgcaagga
     3901 aaaacgggct tgattacaac gtcaagaaga ttgagaacgg caagccgccg acctaccttg
     3961 tgtcttttag gggcaaggat attgacgtta tgaccgaagc gttccgggaa tttaccgcaa
     4021 agaaattagg cagggataaa aagccctcta tccgcaagct gctttccact ctgaaagata
     4081 aggcggcagc tctgaacgca cagagggaca aagtgaagaa aaaggacagg gaggtatcgc
     4141 tatgaagccg gaacttaaaa agctgcttat cctaaatgcc ccctatctgc tctttgtcta
     4201 tctctttgac aaaatcggac aggcggtgcg gctctctccg ggagctgacc tttccggcaa
     4261 ggtgctttcc cttgcagacg gcttttccgc tgcctttgca aacccgcttc cgagccttgt
     4321 ccctatggat ttgcttatcg gtattgcggg cgctgtcctt atccggctta tggtgtattt
     4381 caaaggcaag aacgcgaaga aataccgaaa aggtattgaa tacggttctg cccgttgggg
     4441 caacgccgaa gatataaagc cctacaccga cccggtattt cagaataatg tgctgctgac
     4501 acagacggaa cggcttacca tgaacagccg cccgaagcag ccgaagtatg caaggaataa
     4561 aaatatcctt gttatcgggg gaagcggcag cggcaagacg agattttttg tgaagcccaa
     4621 cttaatgcaa atgcacagca gctacgttgt aaccgatccg aaagggttaa tctattaagg
     4681 acagaggaaa taaagccgca gaaagtgagg tgcagagcat gaggacagca acacagacta
     4741 tgaaattcac agccttgtac gagcgtttaa gccgagatga tgaactcata ggcgagagca
     4801 acagtatcaa aaatcagaaa cagcttcttg aaagttacgc ccataaaaac ggctattccc
     4861 ctatccggca cttcacagat gacggagtaa gcggaacaac tttcgagcgt gagggcttcc
     4921 aagccatgat tgcagaggtt gaagctggaa atgtgagcgc agttattgta aaggacatga
     4981 gccgctttgg gcgtgattat ctgaaagtcg gtttttatac cgaagtcatg ttcaaagaaa
     5041 agggagtgcg gtttatcgct atcaacaacg gcatagacag cgcaaaccaa caggacagcg
     5101 attttacccc gttcttaaac attatgaacg agtggtatgc aagggacgca agccgcaaga
     5161 tacaggcagt tttcaaatcc cgtatgcagg acggcaagcg tgtttcccca agcgttccct
     5221 acggctattt gagaagcccc gaagataaac agaagctaat cattgacgaa gaacctgccg
     5281 ccgttgtgcg ccggatttat caaatggtaa tcgaggggaa aggcattacc gccatagcag
     5341 atattttgac cgctgaaaaa gtgctaatac cgtcagctta tgcaaaaatc cattgccccg
     5401 aaaatgacca cagcaagggc tttacaaatc cttatctctg gtctgctacc gctgtttcct
     5461 atatccttga aaagcaggaa tacatggggc atacggtact gggaaagaca atcagcgtca
     5521 gctacaagac caagaagcgg cgcaaggcag agccggacga actgatgatt ttcaagaaca
     5581 cccaccctgc tattgtggac gaggaaacat ggcatttagc gcagaagcta cgaaaaacgg
     5641 tcagaaaacc aagctataac agaccgcccc accccttgac agggcttgtg tattgcgccg
     5701 attgcagcag taaaatgacg caccggcagc caagccccac caagaaaaag aagtatgacg
     5761 cagacgacgc ttatatatgc ggcagttacc gacagcgcac aaaggattgt accatgcact
     5821 ttatcaagac ttccgttttg tgggaactga ttttaacggc aatacgggaa gtcagcgatt
     5881 atgtgcggca ggacgaacag gctttcatag acaaggtaca gcagacctct atcgtccaaa
     5941 tggcagaaac gcagagggaa cagaaacggc ggcttgcaga agccaccgag cgcaacgaag
     6001 aactgaatac cctaatcaag aagctgtatg agggcaacgc cacaggtaag atacccgaca
     6061 agcattttga acggctgtta gctgaatatg acagggagca gaccgcactg gaaaccgaga
     6121 ttgaggactt acaggagcag attgacagct ttgatgagga caacacaaaa gtcgataaat
     6181 tcatagccgt tgtgcggcga tatacggatt ttacggaact taccacccct atgctgaatg
     6241 agtttataga aaaggtggta gtccatgaag ccacaggcgg caggacagac aggaaacaga
     6301 aaattgatgt gtattttaac tttgtcgggc aggtggaact accgccgccg gacagggcat
     6361 gacaggcagg aaaggcactt cttagggagt gtcttttctc ttgccggaaa ggagcagata
     6421 tgagattgag ccgatatgag aaagaaacca ttatcctttt gaacgccgcc gaggacaccg
     6481 ccagtatcta caccgcagac cccgtatgga aacggaaact tgataaactg gtggagaaaa
     6541 atccacaatg ctatcagtgc gtcaaggctg atgaagtcag caagacctat tccatgccga
     6601 agcgatttat cagtttacgg tcaaaggaaa agaccgttac gctcacagac gagcagaaaa
     6661 aacgggcaaa acagagatta acgaggacag acaaatgagg gcgttttggg ggcgttctgc
     6721 gcttcataag ccctactcca atagcatttt aagaagccgg aaaccgcaaa aggtattagg
     6781 gtatacctgc cccatgatta tgccgtttgt gctgtttgag gtgggaaagg aggattgatt
     6841 gcatgagttt tccgtacttc tacggcagag ccgccgagca gtacgccttt taccgcatac
     6901 cgaagctgct gttttctgat gaacgctttg aagccctctc cacagacgca aaacttttgt
     6961 atggtatgct gatagaccgc atggagcttt ccatgaaaaa cggctggacg gataaggacg
     7021 ggcgtgtgtt tatctacttc acactggagg acgcaaggga acggctgaaa tgcggcactg
     7081 aaaagattgt caggctgttt gcagagttag acagcgagaa aggtatcggg ctgattgagt
     7141 gtgtccggca gggcttagga aagccgaata ttatctatgt caagaacttc atggacgata
     7201 cttccctcta tgactggtag gtatgggagg gaaagggttt gggaaaggaa aggattgata
     7261 gataagtctg ttatacttgt attgatataa tcagttttat acacttcgat aaccgaaatt
     7321 ccagaacttc ggaaatcgaa actcttgaac ttcgataatc gaaagtcaag aactacggaa
     7381 accgaaatac agaggtattg actttcattt ttgctcgtgg tatatttata acatgaagat
     7441 agaaatatac cacattcaga gcggaggata ttttatgaag aagatgaatt atttaggact
     7501 tttatcatta ctttctctaa ttgctatatt agggtggaaa actggaaata ccggattgta
     7561 tggatttttt ggttttgcct attacattcg ttacttttgg gttattccag atgagctatt
     7621 tcaacaaaat gttcggaaat ctactacttt tgcctttttt gcagaaatga tttcccttgt
     7681 tccgttcatg tttatttgtg caacgcttta tgatagttct tctgctattc ctatggcttt
     7741 tggactaagt ttcgctactg ctattcttac atttacagtt gttttaatga tacaggaatg
     7801 gaaagaacga aaaggggcgc aggaatgata aaaaatagga ttaaggagta cagagccaaa
     7861 catgatatga aacaggagga cttagccaag ttagtcggtg tacgccggga aacgataggc
     7921 aatcttgaaa aaggcagata caatccgtct ttggtgttag cgtggaatat cgcaaaagtg
     7981 tttaatgtga caatcgaaga aattttcaca gtagagtaaa agaaagaagc gttttttggt
     8041 atgccatatc aaatgacgct tcttgttgtt tacaggagta ccgttgccgt tccccacctc
     8101 tcctgtctgc tttgcgtaac tatcacacga acagacagga ggaaaatcaa aatgaatgaa
     8161 aacatagaaa tcttacaacg ccagtttgac gcatactgta cgaaagtctt gaaatatgct
     8221 tctatcagct actaccacgc caaccgccgc cgcatggagc atgaagtgtc cttttcttcc
     8281 ctactggaaa aggatttcgc cgaggtcagc accacagacc gctacccttg tatgctttac
     8341 cgtttccaag tgagggaatg gcttgtagag gtgcagagtg agccgttagg acacgccata
     8401 gaacggctta ccccatacag gcaggaaatc atcttactgt actttttctt aggtttcaag
     8461 aataaagaga ttgccgccct ctatggctgt aatgccggaa aaataagcta ccacaaaatg
     8521 agggctgtcc ggcagatacg ggcagaaatg gaggtgcgcc gccatgacta aaaaatacaa
     8581 agaccgctat gttcccgttg aagtaatcat agccgccaca cagggggaag ccgccgccat
     8641 tcagacggta ctgaaaactt atgaaagcta tatccgcaac aaatgtatca agacggttta
     8701 tgacgaaagg gggcttgtgt actacgggat agacacagat aaaaaagact tcatggaacg
     8761 ccgcttaatt catgctgtcg tggagaattt caagatagac agataaggcg aaagtacaag
     8821 acaggggcag tcagaaaaaa gctgattgtc cttgtcgaat agggatagga aacacaagcg
     8881 aggcatggta aaataaagac atcaaaaacg gagtttgtgc ggaggtgtta aaaatggcaa
     8941 cttcaaatat taaagccagt attccttgtg atattcatag cgtatggaaa gttgttacag
     9001 aggttaaaaa ttacacatgg cgtagtgatt tgagtaaaac agaagtcata agcgacaagc
     9061 agtttatcga atataccaaa gagggctatc cgaccacctt tacaatcact attacagaac
     9121 catataagcg ttgggaattt gatatggaaa acagcaacat gaaaggacac tgggttggta
     9181 tttttacttc caaagatact gaaacggaga ttgattttac agagtatgta acagcgaaaa
     9241 aattatttat gaaacctttt gtaaaatcct atctaaaaaa acaacaggcg caatttgtta
     9301 ctgatttgaa aaaagcaata ttatgcttaa tggaggaaca aaatggaata taaagtaaat
     9361 gatataaaac ttgttgcttc aactttcatt ccctttgtca atcaaatatg gaaaggcaac
     9421 tatgatatag agcgcacaca agccgcatta tctaaaacgc ttaatattac agcgtatgat
     9481 aataaaatac ttgtagggtg tttgcgtatt cttacagacg gatatttttt cggaacaatt
     9541 acagaattac ttgttcttcc agagtatcaa aaacaaggcg taggcagtaa acttcttgaa
     9601 cttgcaaagg agaacactcc aaccatgttg tattttggcg cacagccggg tgtagaagca
     9661 ttttacgaaa aaaacggctg tcaaaaaagc ctacaatctt acataataga aaaacccaag
     9721 gaacatataa cggaataacc gctaccctca cagtagcacc accgagcagg aaatcccgaa
     9781 acggacttcc tgcttttctt atgcccgaaa tcagaaagga gattttaacc atgacagaca
     9841 ttgaagaaaa gattgcaaaa gccgaggaag aaatccggca gttacagaac agaaagcgga
     9901 aacttctcaa tcagaaaaaa gacgcagaac gcaaggcgag gacgcaccgc cttattgagc
     9961 gtggggcgat actggaaagc ctgttggaga agccggagca atacagcaac gagcagataa
    10021 aaggcttgtt ggagattgct tttcagaccg cacaggcaca ggagcattta cggaaaatca
    10081 aagaagaaaa cggggagaat taaatctccc ttgcttgaca agggcgcact tatacacctt
    10141 tacaggtgtg tgcgctctgc cgagggctta tatcagcgtt ccgctgatac aggggagcta
    10201 cactcccctg cgggaatttc attccctacc ccttgtgtac ttcacgccag acgataccct
    10261 gctttgcagt ctatcgtctg ccatgaagtc tgaatatgcc gcccgaaagg aggttgacag
    10321 cgtggcgata tatcattgca gtattaagat tgttagcaga gggaaaggca agtcagccgt
    10381 tgccgccgcc gcttaccgaa gcggagaaac cctcacaaac aactatgacg gcgttaccca
    10441 tgactttacg aggaaaggcg gtattgtgca tacggaaatc ctcttgccgc cccatgcgcc
    10501 gccggatttt gcagaccgtt ctaccctatg gaacggcgtg gaaaaaatcg agaaagccaa
    10561 gaactcacag cttgcaaggg agattgaaat tgccctgccc gtagaacttg acagggaaca
    10621 gcagatacag cttgtacggg aatatgtgaa agaaaatttt gtgtctgttg gaatgtgcgc
    10681 cgattttgcc attcacgata aacaggacgg aaaccctcat gctcatatca tgcttaccat
    10741 gcgcccgttg gagcagtccg gcgagtgggg agcaaagtca aaaaaggaat atcttttaga
    10801 caaggacggt cagcgtatca agttgaaaaa cggcacattc aagagccgca aggttgatac
    10861 ggttgactgg aacagccaag agaaagccga agtatggcgg caggcgtggg cggatattgc
    10921 gaaccgctat cttgccgcac aggacagacc ggaacgcata gaccaccgtt cctataagcg
    10981 gcaaggtata gagcagatac cgaccgtcca catgggcgtt gccgctaccc aaatggagcg
    11041 caggggcatt gttaccgata aaggcaatca gaacagagaa atacgggaac aaaaccgtct
    11101 gttaaaggag gtcaaacgcc ggattgccgc cctcacagca tggataaaag aaaagaccgc
    11161 acagacaaag gaaaatcctt tcccttccgt tcctatcctt tccgagcgtt ccgaacagcc
    11221 gcccaccctt ttagaattgc ttaaccgagc ctatacagaa gccgccaagc cacagagccg
    11281 gtacggaaaa atccaagact tgaagctgta cgcaaaggca tttaatttct tgcagagcag
    11341 gaacattact acccttgtgc agttgcagga agttgtatcg gatatgaaaa aacaatactg
    11401 gactaccaac ggggaaatca agcagacgga aaagctactc catgagcgaa aggaactgat
    11461 agaccaagcc gagaaatatt tagaacacca ccccacctac aaggcgtata tgcagaccaa
    11521 gcccaagaag caggaggatt ttttcgagag caaccgagcc gcattgattt tgtatcagag
    11581 tgctgagcgt tatctcaaag agcatttagg ggaagataag gtgctgaagc cgaaagcatg
    11641 gaaagccgaa gttgccgacc ttacccaaaa gaaaagccgc ttgtatagcg atttgcagac
    11701 cttgaaagcg gaggtgcagg aagccgaagc cgtcaagaaa tgcgtggaac aggcagtaca
    11761 gccggagcag accaaagaaa agacgcagaa caaacggcgt gatatggaat tgtgattgtg
    11821 acggataagt gataaaatga tgatatgatt aaaagaaatt aaggagagtt gtgttatgga
    11881 atataaaata cgacaaatga aaaatacgga atatccatta ttgaatgatt ttttgtatga
    11941 agctatctat attccaaacg gtatagagcc gccgcctaag acagtaatat attcccccga
    12001 attgcaggtg tatgtttctg attttggaca gatgaaacat gatatagctt tagtcgcaga
    12061 aatagacaaa aaaataatag gtgctgtttg ggtgcgtatt atgaacgatt acgggcatat
    12121 tgataatact acaccctctc ttgcaatgtc tgtttttaag gaatatcgag ggttgggagt
    12181 aggtacagca ttgttagagg agttaattgc aactttgaaa tcaaaaggat attcccaagt
    12241 atcgttatcc gttcagaaag ataattatgc agtaaagatg tatcaaaaag ttggatttac
    12301 gattttgaaa gaagttgatg aggagtatat catggtaatt gctttatagt tacgcagtag
    12361 gcggcttgaa acacctattc cacaaggctt tttaagtatc aaaatcacaa agacggtatt
    12421 ttatatctct accctcaaaa acaggcttct attgcgtaac acaaccattg aaaactgaat
    12481 aaccgctacc ctcacagtag caccatcgag taggaaatct tgaaacagac ttcctgcttt
    12541 ttcttttgcc aaaatcagaa aggagagcct atgcaggaaa acacacagac agccactaag
    12601 accaccgccg ccccgaagcc gcagaccgtc aagcggaaaa tcggaaagac cacctatgaa
    12661 ttatcgcttc atttcagtca gacaagtaca gagcgcatga gtgataaagt catgcggcta
    12721 ctggaaaatg acctatccgt aaaaaaatga ggaaaacttt tctctatgtc cttgacaaac
    12781 agaccgaaaa ggtacggttt tagtcgagtg cgggaagctc ttacagcgcg gcgggtatcg
    12841 gataaaggta ctgaacacga taaattt
//
LOCUS       DAEMIK010000129        12523 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEMIK010000129 DAEMIK010000000
VERSION     DAEMIK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.130"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(265..708)
                     /locus_tag="KPK87_002977"
     CDS             complement(265..708)
                     /locus_tag="KPK87_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539101.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(711..908)
                     /locus_tag="KPK87_002978"
     CDS             complement(711..908)
                     /locus_tag="KPK87_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539102.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(1100..1966)
                     /locus_tag="KPK87_002979"
     CDS             complement(1100..1966)
                     /locus_tag="KPK87_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2539103.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(2192..3061)
                     /locus_tag="KPK87_002980"
     CDS             complement(2192..3061)
                     /locus_tag="KPK87_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2539104.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(3567..3788)
                     /locus_tag="KPK87_002981"
     CDS             complement(3567..3788)
                     /locus_tag="KPK87_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539105.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(4289..5269)
                     /locus_tag="KPK87_002982"
     CDS             complement(4289..5269)
                     /locus_tag="KPK87_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539106.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(5433..6305)
                     /locus_tag="KPK87_002983"
     CDS             complement(5433..6305)
                     /locus_tag="KPK87_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2539107.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(6469..7020)
                     /locus_tag="KPK87_002984"
     CDS             complement(6469..7020)
                     /locus_tag="KPK87_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539108.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNESTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(7040..7942)
                     /locus_tag="KPK87_002985"
     CDS             complement(7040..7942)
                     /locus_tag="KPK87_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG2539109.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7957..10329)
                     /locus_tag="KPK87_002986"
     CDS             complement(7957..10329)
                     /locus_tag="KPK87_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG2539110.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(10550..11239)
                     /locus_tag="KPK87_002987"
     CDS             complement(10550..11239)
                     /locus_tag="KPK87_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2539111.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(11480..>12523)
                     /locus_tag="KPK87_002988"
     CDS             complement(11480..>12523)
                     /locus_tag="KPK87_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2539112.1"
                     /translation="ILFFAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSF
                     FRKSIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPK
                     LVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDIN
                     NHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIK
                     FTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVL
                     KCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNI
                     YIPLDQDTDSWGD"
BASE COUNT         4119 a         2186 c         1201 g         5017 t
ORIGIN      
        1 tggaattggt agacgcagtg gactcaaaat ccaccggact aatactccgt gccggttcga
       61 gtccggcctc gggcaccatt aaaaactcct aaagctattt attcaataga ttaggagttt
      121 ttttattttt gatttaaatt ttaagttatt ttttgaaatt actgtgtctt tttaaaattt
      181 atataattat ataaatttat cttctagcat taatttaaaa actaatttat ttttatataa
      241 ttttctattt tatatcataa cttattacaa ctttttatct aatttattgt atacaacaac
      301 actacaaata aattgaaata aaataagcaa tccaagaaca actaaaaccc acttgcttat
      361 taccattaca gaaaatacac tcaatataga aacaataaat ataagaatta caagaacata
      421 ggacatgatt tgattagtga tacgtccagc tttatcacgt aacataattc ttctttcatc
      481 attcatctct atcctttgat tttttatctt ttcttcataa tctactgctc tttcaggttt
      541 actccaatac atatattgaa gaatcataca tacacctgga cctaaagcag ctccagataa
      601 tccccaaata aaagcctcaa atgaaaactc agtaaatgta gccaataaaa caagtaaaat
      661 tccacaaata aaatatataa ttccaacata taacatattc tttttcattt ttactcctcc
      721 tcataaataa atatttcttc aatactcata tcaaagtatc tagctatctt aaaggctaag
      781 atgatagaag gattatacct accattttct aatgaaccta tagtttgtct tgatacttgc
      841 aaaatagaag caagttcttc ttgttttatg cctctttctt ttcttatttc ttctaatcta
      901 tttttcacat ctatccccct ttaatatctc aactacggaa agtctacttt ccataattat
      961 acaactatat tccatttatg taaagcttac tttccatatt tacaaaaaaa taacagacta
     1021 ttttcagcct gtaattttag aatttcatgt tgtttattaa ttttaatgtt atttattaat
     1081 tccatattat ttattaattt taacttggtt tctgaattgg aatccaaact tctgtagtat
     1141 aatcaggagc attgatatcg ccattgggat aaagttcaat atctggtaaa ttagcataca
     1201 catacccaga acttggtaac cactcagtaa taattctttt ttgcaattcc tgtattgcca
     1261 gagcatttgg cattggagaa acacattcaa atactgccca tgtgcctgat ggtattaagt
     1321 attcatgagt atcttctaat acaggcttat ctgttggagc agcaatataa taatctaaac
     1381 ccttactatt tgtacacaca ctaactccaa gcaatccaca aggttcatta tttattaact
     1441 ctgaaagttt atcaattgtt ccatttttta tgcatttagc ccacaactta ggaactttct
     1501 caaagcactc ttctacaatc atgttcatac tttcttttat accaacaata cgaaaccctt
     1561 tcttttctac aatacgatat tgcatttcta tatttccttt tacataaatg cttaatgtca
     1621 tcttaggata tgcttttaaa acaacaccct ttgctctagc tgcagatgga gaaatgttat
     1681 gtatattttg aaaagctcta ttaaaagatg ttggtgaatc gtaaccatac ttctcagata
     1741 gttctaaaat tttgatgtta ctgttctgaa gttcaaatgc agccttagtc atacgtcttc
     1801 gtctaatata ttcagataat ggtacttctg caatatatga aaacattctt tgaaaatgaa
     1861 aggttgaaca acatgcaatt tttgctgctt ctgcataatc cactccactt tcgagatttt
     1921 cttcaatata agtaatagct tgatttaatt tatttatcca ttccatttga tattacctcc
     1981 aagcaaatta tacagtttaa taaaataata tacctctcta ttcatgcaca aaattgtaag
     2041 catttaaatt cataagtatt tttcaatcaa tacaataaat ataaaaattg tattatacaa
     2101 taaaaaagtt caataacatt atattataaa atgttattga actttttata aaaattaatt
     2161 taagtgtagt taaattaagt attacactat cttatatatt acaatatcct actatcttat
     2221 agtcttctgg cactttataa tctttgtctg gagtacctaa attccatttt aaatcaaaca
     2281 tagttgcact tattattgtt gcaaaataaa gcataactat accaatgtct attttttctt
     2341 catataaatt agtatgacca tcttttctaa cagctaaaac tacagtgcct ccatcaacta
     2401 taaatctcca tggttgtcta tttaatgtag atggagctaa tcttgcatag tgaaatgcat
     2461 caagaagtgc tctttcttct aagatttcta tacttgcaac atttccccat tcgttcatat
     2521 aaacaacatc ttctacacct agtctagtag accccttatc tgttacaact tgcatatcag
     2581 attttgaata attttgccca gtttttgtag catttaatac tttagcatta cttacatttt
     2641 ctccatatcc aatagctata attcctgtta cctctttatc tgaattaatt ccaagttttt
     2701 cttttacctt ttttccatct tcaaaagtaa cccaacaaga accaattcca agttctgtta
     2761 ctttaaagat tatactttct cctatgtacc ctgaattttc aatgtaatta tcttttatgt
     2821 cagataatat tataatataa cttggagaat ctatcatatt tcctttgtac cctgctactt
     2881 tatctaaatt attaaaaact tcacttctat tcataattct tacatcaaca tctatttctg
     2941 gaactagttt cttacaagat tctgagtatt tcttaatttc actaagatat tcttctttaa
     3001 tatctgtgtt cttatattct ctaacagact ttctatttaa tatgattgtt ttataatcca
     3061 taattttatc ctccccaaac tagtattgtc ttaattatat acccattaat atattatttt
     3121 actcatttat ttaataaata aagattacaa ttttgtaatt attatttatt aataatattc
     3181 cacttttaca ttaataatga ccagttgtgc aaaatccatt gccaaccacc aaataaaaac
     3241 atattataaa ggtaatataa aataacaaac aaatcttaac aaaaaattaa acttttttta
     3301 cacgaaagaa ggcttcccgt tgccttcttt atttttataa aaaaataagc tgtttctaaa
     3361 atgatttcac atttttgaaa cagccaagtt attctataat gacaactata atttgaggat
     3421 gtgagggaga gttgtcatta taaatttagg gtaattatat tatgttttaa gctagttctt
     3481 tctatattta tataatacat tttatatatg aattttatat gagattggta taaatgatat
     3541 atgaaattac atatgtttat attatattaa attttattat tacattttcc acaacaatac
     3601 tcatatgtct ccacattatt tggaatattt acaaatacaa attctttaca gttctcacaa
     3661 tactttaata tagatatagt tccacaagtg taacattcaa atattacaca atcctcattt
     3721 tcctctacta tttcaaactt tttactatgt tcaccacact ctgggcactt gtgattattt
     3781 acattcataa atactcctcc ttattagtat agttacatgt caaaaactta attaatgtat
     3841 tctatattac ttattatata ttctataata aatcatattc taatataata aaacaaatta
     3901 aataaaaaaa aaccataaat tttaaattta tggattaatt cttgtaatga taactctatt
     3961 tgaggatgtg agggagggtt atcattacaa tagggtatta tattatggtt tttaagctag
     4021 tctttctaca cctcatatga tacatatgct atatgaattt tatatgattt ttacataaat
     4081 attttatgaa atttaaattt tttagtcatt ttgaaatttg taaaaagttc tctaaaaatc
     4141 cacagcatca taaacagcca aaatttaagt aaaacatcat aaaaaaattt gtccaataat
     4201 tttaacaagt aatctcacct cattataatt attgacacaa acttaaataa acaatctata
     4261 aatcttctat aaatcttaaa ctttccccct attgatacac aatttctagt ttgtcaattt
     4321 tattttgcat ttctaagaaa atttcttcaa cagaattgtt aaatatattg cctaaataga
     4381 ttcttttatc tttttgaaat gaataagatt ttacactacc atcagaagaa atatataaat
     4441 gactccactc tgctccatat tttgaaaacc tctcctttgt atctttaaac ctcatatctt
     4501 ctatttttat tgctttttct ctgtctcctc ttactacagt gtgacccctt tttattaaac
     4561 tggttatttt tatatcttct tttaatttaa tacgtgtatc atcatttttt gaatatactt
     4621 tccatatcac tttatgacga taattatcta attcctttat taagcttcta tcaatagtaa
     4681 aactatcttt agttaccaca attaaatcaa aatggtctaa catatattga aatatttctc
     4741 tcatactttt attttttaaa atatcctcac caataacctc tataaatcta cagcctttct
     4801 catacatgca gttcaataaa cttgtgattt cagaaaaata atcatttgta ttattatctc
     4861 catctatttc tatagtaaag tacaaaggag taatgtatga tttatttcct gttatctcaa
     4921 atacacttga tagtgtctgt atatcatctt caaacgttaa gtatacattc tcataaaaca
     4981 taaaaaattc cataactttt tctttcatat tatggatatt tctatatcta ttggataata
     5041 tatttattat atcgtcagta gtatttttcc catcacattc agccagtata acaatttgct
     5101 tccaacttaa gctatccaca tttccgatac tttttttata tccatcttct tcatcataaa
     5161 tagggccaat tataatgttt tcttttggcc acagatgaac cctagttcca tcttttaatt
     5221 tcggatattt attttttaca gactcataaa atctacttgt atcacccatg attctttact
     5281 accccccata gtttaaatat tctatgctaa aaatagcact ttatttttat taattcaaca
     5341 attattcaaa attggtcatt atatatgttt aaatggattt aacatcaacc ttatttatat
     5401 gttattgatg tttatatatg ttatataaaa tcttatttct catacttcaa tgtaatctta
     5461 actatctctg gtttacaatt aagcctaatt aaaacttttg agcctcctaa gcctttattt
     5521 aaaatcatag tgctattatc tttttcatat tttccccagt catacttagg aaagaactct
     5581 ctatttggag ataatacacc tccaacaaaa ggtattctta ttatacctcc atgaacatgt
     5641 cctgctaaaa ccaaatctgc tccccatttt gcatattttt caaagaaaaa tggtgtatgt
     5701 gctaaaagaa tgttatattc attttcatta actttaccta gattgtcttc tacaaaattg
     5761 tcacttagtt ctaatttttt gttattagca aataaatatg gatagcattc caatggtata
     5821 attaaaccat ataaattaag atggctatta cctctatata ttcttacaga ttcattttcc
     5881 atatttatta ttttctttga atatagttcc ttaaaataat ccttatatag atgtttatac
     5941 ttttttatta aagatttttg ttcatgattt ccaattatat gatatacttc atatttttca
     6001 accaaattat ctattaaatc taatgcaact tgaaaatttt tattttctcc atctactaaa
     6061 tctcctgtta taaatataac atctgggttt aatgattcaa ttttatctat caatacctta
     6121 ttatttttat caaacacctt attatgtaaa tcactcactt gaactatttt aaagttatca
     6181 aattcctttg gaattttttt gttgaaaata gtatattctg tcgtttctat atgctgagtt
     6241 tcatacttat acagaatata caataatatt aatactaata ctgtcattga aacctttata
     6301 atcatattct cttcctcttt attctatttt gttaatatta ttataatgct tttatgtttt
     6361 ttttaaaaca ataacatcat cacatacata taaatctttt cgacattata aaaataagtt
     6421 atccttttct aaaattttca tttattttag aaaaggataa ctataaaatt atctataaac
     6481 ctctatattg tgaaatatag tttttccttt atttaaattt ttatatatat gctctaaagt
     6541 tccatcaaac ctaatagaat gtccagactc taaaatataa gttttcccat ccacattaag
     6601 gtcaagttta ccttctatta cgagcacata ttcttctaaa acatgactat gtgtagatga
     6661 aacatgagta cagttttcct caagctcaat tataaaaagt tcaaaccttc tttgagggct
     6721 aaaagggtat attggatata acttcatgtt ccctttttgc tcaacaatag gctctacttc
     6781 ttctttcttt ataacctcat aggtatttgt actttcattt aatagtgtag taaaatttat
     6841 tttaaatcca gatgaaattt tccataaagt agatactgtc ggacttgatt cacctctctc
     6901 aatctgtcca agcattgctt tactgacacc tgtcatcttt gaagctttgt ctaaacttat
     6961 gcccatttct tgtctcaata accttaaatt ttctcccaca ttaggaaatt tattatccat
     7021 gttaaaatcc tcctgatagc tatttgtatt ctatgatttt tactcctgta agttctacta
     7081 tctcttttaa ttttaacata tgattgttac aaatttcatc aaattcatat atataatctt
     7141 caagccctaa tgccttatat ttatcgattc caaatttatg atatggaagt aactccatct
     7201 tgcctcctgg aacattttgt ttaacaaata atgcagtatt tcttatattt tcttcactat
     7261 catttactcc ataaataatt ggcactctta taaccataga tttattcaac tttgaaagcc
     7321 tacatatatt atctaatata attttattac ttactcctgt atattcttta tgaatattat
     7381 catccataga ttttatatct acaaatatgt ggtctatttt ctcaaataca tcatttacct
     7441 tattccaatc aaaatatcca caagtttcaa ttgcaatatt tatgccttta tcataaaaaa
     7501 tatcaactaa ttctcttaaa aaatcaatct gaagtgtagg ttcacctcca gaaaaagtta
     7561 tccctccatt agattcaaag aaaaatataa aatctttttc aacttcctct actatttctt
     7621 caacactcat ttcttcggtc attatacaaa tggcatcttt aagacatacc ttgatacact
     7681 ctccacataa atcacattta ttatttattt gcgttctatc aaatagtgaa cttatatttt
     7741 gaggacaaac atctatacat ctattacaag atacacattt atctttcata acacctaatt
     7801 ttactatgtt actccaggaa tctggattag aacaccactt acattttaat ttacatcctt
     7861 ctaaaaatat agtagtcctt attccatctc catcatttac tgaaaaatgt tgtactttta
     7921 caactctacc ttttttactc ataatctacc tcataattaa aaattgctat gtgaatttct
     7981 agctattata gcatcttgca tttctcttga taaattaaca aattgagtac tatatccagc
     8041 aaccctcact aataaatctt tatattcttc tggattttct tgagctttta atagagtatc
     8101 atttgatatc gtattaaatt gtacatggaa tcctcctaaa gcaaaatatg atttaatcat
     8161 agatgttaga tttctaagtc ctctttcagt ttccaccaaa tcttgattca gtcttaaatt
     8221 tagaagtgtt ccaccagtat gaacatcatg atttattttt gatgcagatt tcattgctgc
     8281 tagtggactt gttgtatcag taccaacaaa tggagaaact ccctctgtca aaggttttgt
     8341 cgcttttctt ccacaaggag ttgcacctac tatttttcca gttggtacat agtttgatat
     8401 acccataaat gcactattaa atttagagcc aaaaatatcc ttgtattttc tagtttcagt
     8461 ataataaaaa tcagaaactt caattgctaa tgaatctaca taatcattat catttccata
     8521 cttaggaaca tccaacgcaa gttttcttaa ttcttcataa ccttcccaat cattatttag
     8581 tgcttttgtt aattcttcta acgtagtcac cttatcctca aatactaatt tttttatagc
     8641 tgccaatgaa tttgaaacta cacctaaccc tataccagtt aatacaggac ctatattata
     8701 ttttgctcct cctttactta aatcaactcc cttatccaaa catccatcta cacaagtaga
     8761 taaaaatggt cttggtacca tttctttatg aatttgttga gcaacagtag ttcctataac
     8821 agaacttttt acaagatatg ctaattgctt taaaaactca tccttaagtt cttcaaaaga
     8881 agtaaattct gtgatatttt ttgtatctaa ccccattttt tctccagtta atctactctt
     8941 accttcattc atagcatatt ctaaagctgc tgcaaaattg atatttacgg ctgaagtcca
     9001 ctgtcctgtt tttctaaaat gaggaactac acaaccacag ttactccaat ctctagcatc
     9061 ttctgcatca tatccatctt gtaataacat ttgtgcccct gcactatcgc tgtgtatagc
     9121 agggaaacca gttccttgtt ttaccaattt agcaactgcc attacgaaat tgtctggtgc
     9181 tccttgactc actctaacac ttaatcctgg ttggtgagtt tttacacttt ctgtagcctt
     9241 taaacacata tatgagatat cattcgttcc atcagtacca tttctctttt taccaccaac
     9301 agtaagattt tggaattgat tatatcctgc aaagtaattt gctgtatttg ctgatatagt
     9361 ccatacccat tctgataact taatccaaag tgcttctatc aattcttgag cttctgattc
     9421 agttatttta ccttctctag catcattttc ataatatgga tacatatatt gatcaaatct
     9481 acctaaattt aatgccaatg gattttctga tagaatacca cctaattgaa caaaccatac
     9541 aaattgtatt gcttcataaa aattagttgg agggtttgct ggtacattca tacatatctc
     9601 tgaaattttt ataagttcta actttctctt ttcatctgtt tcttgcttac tcatctcttt
     9661 tgcaagattt gaatatcttt gtgccaatgt cataataccc tgtgcaacta tagaaacaga
     9721 tttgtaaaaa tctatctttt caatattttc tgaaactgat atatctaatt ctttaatttt
     9781 tttatctgca tcttccttaa tccctttata tccttttaca aacaatatat cttgataatc
     9841 tggtgttact tcaccaactg cctgtctcca cttagaatca ttatctatta ttcctgtatc
     9901 tactaatatc ttagctgtat cttctgggat tcttgctaag aaaacttctt ccaaagattt
     9961 tcccttccaa tatggaaata tttcttttct tataaattct atttgttctt ttgatattac
    10021 atatgggtct tgagttcttt tatcaaaagt atccatttct ttgtctaccc attgccaaga
    10081 aaactctggt gtcaatatcc ctgttcttct aaattttcct gctgtaccta ctattaattc
    10141 attttcaaag atttttatag gaagttcttt acatgtctcc aaaaaagctt tagctcttct
    10201 tatacagata gcttctccct cagttttttt atgtgattct gtaaaaattc tagccctttc
    10261 acaacatatc ataggtttag aattaatata ttcttgtcta aatgcttcta ttctattagt
    10321 ttgactcatt taaaatcccc ctttggataa tatatactac atttttgtat gttataacat
    10381 actttaatta tattatactg tcctttagca taaaatggaa gcaaaataac ttattttttt
    10441 tcatctacct tgaacaccta tatattaata ctttttttgt taaatttata aaatctaaaa
    10501 aaattttcta tgatatattt tctatattgt tatagttatg tctaatatac taatgatttg
    10561 aaatatcaaa tttgtatact agacttagtt ttaaaacctt agttgattgt ttaactttgt
    10621 taacttcaac atgtatatcc ttcatataag gaagctctac aacacctact cccttattaa
    10681 agtcttcttc aaccatagaa aaaatttctt tctcagttaa gttcgtatca gtcaatgatt
    10741 taaataaatt gtatataaat ttaacatatt tatcactctc agataactta tcatcttctg
    10801 gatactcttt agcacagaga atatctattg ttacttttcc ttctttatcg tcttcagaca
    10861 tagaatagaa taaacttcga atattttttt caggtggttt ttcagcacca tttatagata
    10921 ctacaaagct accatctgat tttgagttag taatactata accactgtct attatagctt
    10981 tcgaaaatct ttgagataaa tcttttttta cttcttctct acctttcgca tcattaatct
    11041 tttgtgagta attattaaat agctcaatag attcatcttt tttatcttca acttgttctt
    11101 tcaatgaatc tttactaatt tgttctgagc tacttaagtt cttatcagat acaggagaca
    11161 tacaccctac catcaatgct aagctaagat ttaaagccac aattattgcc actctattta
    11221 tattcctttt tactttcata cacaatcctc ccatcacact aaccgctcta aagtattatt
    11281 cattgcacta gaatatattt atattttctt tatattaaag atttattatt caatacatat
    11341 caccttccta tccaattctt ataacttact attagtaaaa ttataagctc cttaatacag
    11401 aaataaggat gttatctttt gttattaaaa gataacatcc ttattttttt gtatcaatac
    11461 atatgtccca actggtaact taatcgcccc aactgtcagt atcttggtcc aatggtatat
    11521 atatatttag tataaattta tcgatactat tttcaaataa taattctcca ttatattttt
    11581 gtaaagaaga tttaatactc tgaataccta ttccatgtac gaacttatcc attttgtttg
    11641 ttaacaatat atttttatta aaagtaagct gatttagttt actattttca catttaagta
    11701 caaaaaatga ccttgttata gttcctttaa ttctaatata tttattaata ttttcatcca
    11761 ctattttatc acatgcttct attgcattat ccaaaatatt agaaaatatg cttgacacat
    11821 ctataggttt tacaaaattt acttttgaaa aatttataga gcaagtaaat ttaattcctt
    11881 tttttaaaca tacttcactc ttttcgttga ttataatgtc taatatcata tttcctgtat
    11941 tatagatata tttaaatgtt tttatttcat cttttaaatt gttaacatat tcattaattt
    12001 ctttgctatt gtttctaagg ttgtctatac aataaatgtg attatttata tcgtgatata
    12061 gtttcttaac tcgcatatga gattcttgaa tacttaaata atgcgcatat tgcatatcta
    12121 acttattttt tattaattca gcttgagatt ttaatctatt ctcttttatt atttctttaa
    12181 acaatagtat aagaaatata ctactagctc ctaccaattt tggattaaca aaattatcaa
    12241 aatagtatcc ttctgaatgt aatgacccaa accgttctaa tttttttaat acataattta
    12301 caagcatatt cattgtatta actaataagg caaataataa atatacataa tttctcttat
    12361 cttggctaat taattttata gactttctaa aaaagctaaa tataagatat ataagaataa
    12421 ttattaaatt actatatatc tgaattttta tagattctaa gtaaggtact aataaagatt
    12481 ctttagatga aataacatta cctgtaattg caaaaaatag tat
//
LOCUS       DAEMIK010000130        12436 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEMIK010000130 DAEMIK010000000
VERSION     DAEMIK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.131"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            319..1254
                     /locus_tag="KPK87_002989"
     CDS             319..1254
                     /locus_tag="KPK87_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG2539113.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1346..3217)
                     /locus_tag="KPK87_002990"
     CDS             complement(1346..3217)
                     /locus_tag="KPK87_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG2539114.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3407..4333
                     /locus_tag="KPK87_002991"
     CDS             3407..4333
                     /locus_tag="KPK87_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG2539115.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4472..4618
                     /locus_tag="KPK87_002992"
     CDS             4472..4618
                     /locus_tag="KPK87_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539116.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4723..5247)
                     /locus_tag="KPK87_002993"
     CDS             complement(4723..5247)
                     /locus_tag="KPK87_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539117.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAENNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5300..5524)
                     /locus_tag="KPK87_002994"
     CDS             complement(5300..5524)
                     /locus_tag="KPK87_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539118.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5721..6845
                     /locus_tag="KPK87_002995"
     CDS             5721..6845
                     /locus_tag="KPK87_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG2539119.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7319..8143)
                     /locus_tag="KPK87_002996"
     CDS             complement(7319..8143)
                     /locus_tag="KPK87_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539120.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPVGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8491..9546)
                     /gene="ispG"
                     /locus_tag="KPK87_002997"
                     /gene_synonym="gcpE"
     CDS             complement(8491..9546)
                     /gene="ispG"
                     /locus_tag="KPK87_002997"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG2539121.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKRVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9729..10733)
                     /gene="rseP"
                     /locus_tag="KPK87_002998"
     CDS             complement(9729..10733)
                     /gene="rseP"
                     /locus_tag="KPK87_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG2539122.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGKLKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10738..11901)
                     /locus_tag="KPK87_002999"
     CDS             complement(10738..11901)
                     /locus_tag="KPK87_002999"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG2539123.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKEKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKKIGR"
     gene            complement(12065..12268)
                     /locus_tag="KPK87_003000"
     CDS             complement(12065..12268)
                     /locus_tag="KPK87_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539124.1"
                     /translation="MISYAPLWKLLIDKKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         4145 a         2092 c         1294 g         4905 t
ORIGIN      
        1 taacttatat actttatgtg taaattatgg taaaaatatt atttaatcaa atcaaaactt
       61 gatttatata cctggaaaag gtattatatt aaagagtgct taaaatacgc atttcataaa
      121 tagctatata aaataataaa taaggagagt gatgggcaat ggatactagt ccgttgcgga
      181 gagaattttt tacattttca tcacttaata atatctaaaa tcaaaactaa tacatatatt
      241 cagttatata aattagttaa ttaatagata ctattaaatg atgagattta tataaaactg
      301 aataaaggat gtgttgccat gataagatat tacaaaacta tagattcaaa attagagaaa
      361 cttagttttt ttgaagatgg ctgttggata aatcttgttg aacctaatca tagtgaaatc
      421 aacgaaattt ctaatttatt aaatatagat gtagaaagca tagaatctgc actagatgaa
      481 gaagaacgct caagaataga tgttgaagac aatcatactc ttatacttat agatatacca
      541 gtagacgaaa gtgactctaa ttcttctcat tatactacaa tacccttagg tattatacta
      601 actgaagaag ctattgtaac agtttgtgat gcacaaacta agattttaaa tgactttata
      661 gttggtcata ttaaagattt ttttacgttt aaaaagactc gctttttgct tcaaattctt
      721 cacaaaaatg cagcttatta cttgcattat ttaagaaaaa taaataaaat gacaatcata
      781 atagagcgag aaatttacaa atcaatgaaa aacaaagaat tggttcaatt attggaatta
      841 gaaaaatctt tggtatactt ctctacctca ctaaaatcta atgaacttgt tttaaacaaa
      901 atggttagaa ctgctggtat aaagaagtat ccagatgatg aagacctatt agaagatgtt
      961 atagttgaaa atagacaggc tcttgatatg gctaaaatat atggagatat attgagtaga
     1021 attatggatg ctttcagtgc aattataagt aataatcaga ataatgttat gcaaatttta
     1081 acagtagtta cactaatttt ctcaatacct acaataatat caggattctt tggtatgaat
     1141 gtcatcaaca tgccattttc taataaccca aacggatttt ggataatact tcttattacc
     1201 tctataatat gtatagtgat tacatttttc atgtcaagaa ataagctatt ataaaatatt
     1261 aataaaaaag ccattagaat taattgattt acatttatcc taatggcttt tatatattta
     1321 aatctatata atcctataat actttttaac tatcatgttc ttcatctata atcatttcag
     1381 tttcaagccc ttctagcatt gaatctaaac tttcttttat atttagtata taaattggta
     1441 catactcttc ttcatctatt acctcttctt gcaacttaaa tggaggaatt aatacttcat
     1501 caaagtctat atttaaattt ggaaagcctt ctactatctc ttcacaatta tcgtactctt
     1561 catttgctaa agaaatgtac atatctaggc tctttttata ataaagacct gcaagcatat
     1621 actcacactt cttacttcca tatttaatac cttctttata atatttctca gcttctgcgt
     1681 catcatttaa ttttaaataa atttgaccta aattatatat tgattcaaca caaccttgtg
     1741 ctctagcctt ttcataccaa aatttagaat ttacataatc tttatagaac atatcataaa
     1801 ttaatcctac catatgttga gcatacacat tattttcatt ggcaggtgta tcaaacatca
     1861 ttttagactc ttccaattta ccttctctaa aataaattct acctagatgt atacgagcat
     1921 tcatatggtt tttgctactt gccatttcaa aatactttct agcatttttt gtatttccta
     1981 agctctcata tatacacccc aatctataac tagacctcgt atctccattt tcagatgctt
     2041 tcttatacca acttattgct tcttcaatat tatttaaatc ttcatagata tttcctaatt
     2101 tgatttgaca agatacatta tttggaatcc tcgaataata cgaaatcgct ttttctatat
     2161 cttgattctg ataatataaa tcccctagat tttcaaggga acttttgcat ccaacagcta
     2221 tagcatcctc ataatattta atagcatttt cataatcttt ttcttcaaaa tatatacctg
     2281 ccagattatt ttttgcttct gttatatcat cttcagcagc cattttataa taagtttttg
     2341 ccttctctat ctcattttct ctattatata aattagcaag tctatactta gaaagtgtat
     2401 gatttttttc tatggctttc tcataataat aaattgcatc tcccagctct tcttttctat
     2461 catatacata tgcaagccta tactcagact tcacatcatc tctatcagca ctctttttat
     2521 aatacttctc tgcatcttct aaattatcat ttatttccat aatcataccc aagttaaagc
     2581 aacctttcat atgacctgac ttgtacaact gtttatataa acttactgtt gatttattat
     2641 ctttcttttt tccatttaat attgctaaat tatattttgc atcatcatct aaaagcacag
     2701 aacctttttt atagtattca tacgcttcat catctttctc caaaaaagta tataataccc
     2761 ctaaattaaa acaagcttta cctaaattct tgtctgctgc tgtttttaac catttttcac
     2821 accttacaaa atctttcatt ttaaaatggc atacacctaa attattttgt gcttgtaaat
     2881 aacctaaatt tgctatcttt tcataccaat atatagcata ttcatagcca tctaacttat
     2941 agtacatatt acctaaatca aaagcagcat cttcacttcc acttttatat gccattgaat
     3001 accacttttc tgcttcttta aattgttttt tactttcata tatatttcct aaagtatatt
     3061 gagattttac gtaattatcc aatgcagcac tcttatacaa ctctattgcg ctttccatct
     3121 tacccatctt ttcaaggagg gcacccttag tatgtacgac tttcgggtca ttagatatat
     3181 tttttatttt ttttatagaa aattttgtat ttttcattat tcgctaccta cccattttat
     3241 aaaatatatt ctctcaaaca ttattataca ttataatcta caattaatgc tataatattg
     3301 aatttttacc ttttgtcact atattgtaac attaaatttt aaattttata atcattagat
     3361 tttattatgt aattggtata taatgtatat atagggggga cattttatga atttcagcaa
     3421 tatttttatc caagttgcag ttttatttat aattatttta gttggatatt ttgtcagaaa
     3481 attcaactta ttagatgacc actgcacatc taagctttca actttgacaa tgacagtttt
     3541 tttaccatca atgataatta gctccatgca aattaacttt gatagtaaaa tgatacaaaa
     3601 aatactattg ttattattta catctttgat tatgtatata gtttcaatta taattgcatt
     3661 tttattgaaa tacattttaa aatgtaatga taaaaaagat ttgggaatat atcagtatat
     3721 agttgttttt tcaaatgttg cttttatggg ttatcctgta attgaagcag ttttaggtca
     3781 tgaagcaata ttctatactg caatattcaa tttgcctttt aacttatttt ctttcacttt
     3841 aggaatttat cttttatcta aaggcagtac aagtagaggt ttttcaataa aatcacttat
     3901 aagccctgct acaatagcta ttgttattgg acttttttta tttgtaacag gacttaggtt
     3961 accccaattt ataaacgagc cacttgagat gttgggaagt ataactacac caatatccat
     4021 gattatcatt ggaagcttac ttgctaattc ttcagctctc gattgttttg tcaacaaaaa
     4081 attatatata gtcactttta taagattatt ggtacttcct gtgattgtgt attttattct
     4141 aaaaggatgg ataaatgata agatgatttt agctatacca gtagtaattt cttcaatgcc
     4201 tgctgctgca aatactgcta taatggctaa tcaatatgac tccaatataa cactagcttc
     4261 acaatgtgtg ttttttacaa cactattttc agttatatct atacctttta taagtatttt
     4321 actcttatct tgatttattt ttttatgtat tatataataa catattcaat aaattacaaa
     4381 tataaacatt ttattgtagt atattgatgg aattttttcc tagatatttg tcaataatat
     4441 ctatatgtat tatagaatag gaggcttaaa tatggatatg gatttactct ttaaaatagt
     4501 atttgctata gttgctatag gtgtttctgt aaaaatcatt aaaactgttg ctagtttagc
     4561 ttttaaaata gcgttgatat tgctaataat tttatttgta tatagaatgt ttttgtaaac
     4621 atttaaaaat aagtttttta acacaataga gaagtaaaat agattatctt taatgtaaat
     4681 tttttattaa agacactcta ttttacttct ctattagctt ctttaaatta aatttttatt
     4741 atatcttccc ataatataaa catcttcaaa ttttccatct ctgattacag catatttttt
     4801 agttccctct atttcaaaac ctagcttttt atataaattt aatccctttt cattatccac
     4861 aattacttct aatcctactc tagttaacat aatccaatta tctgctatat ctaaaagttg
     4921 ttccataagc ttcttaccta ctcctcttcc ctgataattc tctgcaacac ttattcctat
     4981 ttctgcgcta tgtctcaatc gtggagaaga atttacatgt aacccagcaa aacctattac
     5041 atctttttct ccattattat tattttcagc tacaaacata taatcatttt ctcccaaaga
     5101 agctaacata gcttcagttt tatcgactct gtcactcatt aatgccaaag tatttctcat
     5161 aacactgtat gttcttctaa tttcattaaa agcttgtgca tcttctcttc ttataggtct
     5221 tattgttata tctataggct tactcatatt ggattccccc tcaaacataa tctttatttt
     5281 taattaaaat tttacatctt tattttatac tgttcaaatc tatgtatgct tctacctgag
     5341 gtacatatat catattatct tttatatgtg tacttccaaa ttcttttaat tctgtacctt
     5401 ctttatcctt agttatttta tatctaatgg attccatacc ttcaactact tcagcatgaa
     5461 aaaacatacc ttcatattgt ttaaatctaa aattaaaatt gtctttagta agttttatta
     5521 tcatattttt atctactcct tatataattg atatttttct tattattagt tgtatatata
     5581 ctgcttattc atattaatat attataactt atcatttatt tagtataaag ttataaatag
     5641 atatatttat acttatttta caatctttct tcaattgata tataatatgt ttatataaac
     5701 taatgtatgt gaggtgaaat atgagcaaaa aaacaaaata tttttcttta attctctttc
     5761 ctattgcttt gcttttaaat tttatagcca gcaaagttcc aaatattgtt gaaaaatatt
     5821 actcccaatt catagataaa ataattgtac aaatattgag taaagtttct ggaattttcc
     5881 cattttctct ctatgagatt acaatgtaca taatagttat atctatcttc ttatttttat
     5941 gttgtacttt atctacaatt tttaataaga aaaaaaaatt aaacatatat ttaaaaaatt
     6001 ccattttaaa tatattatcc attgtatcaa tagtctactt tctttttgta gtgctttggg
     6061 gaataaacta taataggatt ccactagaaa ccacacttat aaataactat aatttaaaaa
     6121 acaatacctc tatccaatca aaacaatata gtgttaaaga gcttactaaa ttatataaat
     6181 tccttgtaat taaagcaaat gaaactagaa aactcacatt acaagataaa aatggtattg
     6241 taaaatctaa tacagattat aatggtgtca taaatagagc tcaactagga tacgataata
     6301 tcttggatat attacctagt gttagtggta gttattctaa gcctaagtat attatttcat
     6361 caaatctaat gtgctataca ggaataactg gtatatactt tccttttaca ggtgaggcaa
     6421 atgtaaatat agcaatacct gacttatata ttccttgtac agttgaacat gaaatggcac
     6481 atcaaagggg atttgctagt gaagatgagg ctaattttat agcgtatctt acttctataa
     6541 aacaccccaa tattgatttt aactattctg gatatatttt agctttaaat tatactgctt
     6601 ctgctttaag taaggttgat tataatgcat atgttgatat ttctgctggc atttcagatt
     6661 ctgtccgtag agatttaaaa aatgaaagtg aattttggca aaaatatgaa ggtaaaatta
     6721 atgaaatttc taatgagttt aacaattctt acttaaaagc taatggcgtt actgaaggta
     6781 ctcagagtta tggtaaaatg gttgatttat tgctaactta ttatgaatta tatccatata
     6841 actaaacact acaattcaaa attttattca tatacagctg atattaaatc agctgttttc
     6901 tatttaaata aattatttaa cttcaaacaa tcttatataa tttgatagta cttatactag
     6961 ctgtataaca tttcaattct aactatttta gttaaaacat ttctattatc ttttttaata
     7021 aatgttttaa ctaaaatttt taaaacataa aacttccaca ttatttcact atctatataa
     7081 aaatctaaaa attcacatat aaaattacat aactaaaaaa tcataaattt atagaaaaca
     7141 tatattcaaa ataaaaaagc atgtatcaaa acttgacaca tgcttataat cttatgtata
     7201 tatatcattt tgtcacaata aaaatttcct tgtaacactg atatttttaa acaaaatagt
     7261 ccttatttaa ttaaattaac attaattaaa ttcgtactca ttttgctatc attaaacatt
     7321 acttatagta attttctaat acactatttg aaacttcctt cccttgaaaa cttttcaacc
     7381 aattctcata tgctgagttt ttccatacac taccatcatt aaatgttact tgttttacac
     7441 aagatataat gtactttaca ccatgctctc cagttgattg tttccattta tcaaacaata
     7501 gaaaggcatc attttgattc ttacctggtg ctattgcatt ttgctttgca tattctttct
     7561 ccatttgttt ccattctttc aaccaatttt ctacccatgc cttgccttgt tgtttttcaa
     7621 acatacttat tatatcctgt ggtggtgatt gttgcatttt tctataagta tgtgaatatg
     7681 attttggaga cacaggagat atgcctgtaa caataccata gtctacacca actgggctaa
     7741 atccaacact tcctacttcg ccatctgctg ccacattttg agcatcccaa tatagctcaa
     7801 gtggttttcc atttttatca tatgctaagc atgtaatttc ataatccata attgcctttt
     7861 cactgctatt tgttaagctt ataacattgc taggccaatg atatggagta tcatcatact
     7921 gtattttgct attatttata aataatggaa tattctcatt ataaacaaga cctttactac
     7981 tttgaatatc tgatactttc tttgtgacag tacttaccgt atttgctaag tatacatttg
     8041 ttttttctga attggtcgta ctaagagaaa atgttgttgt cgttccagta atcagcaaag
     8101 caacaactaa aataggtaag ctagatattt ttttatatct cacagttctt cacctctttt
     8161 tctattaact tataataaaa caatttacca aaacattaaa atctatgtta acataattta
     8221 ccaactgttc tagtaactat atcctatcat agttactatc aagtttcaat actattgatg
     8281 gaaattttca ctatattaaa tcttttttta attttataaa tttacaatat gatttaactc
     8341 atacatatat agtcaaatta gaatataagt ttcttacatt taatgaagtt tataaaataa
     8401 aaaatgacta gataattaaa tactgtaaat aaaaacttat tacaaaagta tttaaaaatc
     8461 tagtcattta atatatatat gaattgtata ctataatttt tcaatctctt ctagcaactc
     8521 atcaactaat ttgttaccat caatcattct aactatttca ccctttttaa ataaaagacc
     8581 ttcaccttta cccccagcaa tacctatatc tgcctctctg gcttcaccag gtccatttac
     8641 agcacagccc ataattgcaa cagtgatatt tttttccata ctaccaatct tttcttcaac
     8701 ctcatttaca acactaatta agtctatatt acatctacca catgtagggc atgatataac
     8761 tttaatctta tcatttaaaa gtcccaaact tcttaaaatc tgtttaccaa caataacctc
     8821 ttcaacaggg tcgcctgtta aagatattct aacagtatct ccaattcctt taagaagaag
     8881 tgctcctacc cctatagaag actttattgt tcctttgtga acacttccag attctgtaat
     8941 tccaatatga agaggataat caactctttt tgatattaat tcatatgcat caacagtttt
     9001 ataaatatct gatgatttta gagatataac tatattatga aaatctaagt cttcaagaat
     9061 cttaatatgt cccattgcac tttcaactaa tgcttcagca gttgcagagc catacttttt
     9121 taaaagttct ttctctaaag aacctccatt aactccaatt ctaattttta aatttctttc
     9181 cttgcatttc tcaactacca tcttaactct ctctatactt ccaatattac caggatttat
     9241 tcttattcca tccacacctt gttctatagc ttcaagggct agcttataat cgaaatgtat
     9301 atctgcaata attggtatat ttacactaga ctttatcttt gctatatttt tagcagcttt
     9361 aatatcagga acagcaactc taactatatc acaaccagct tcctctaatc tctttatttg
     9421 agctattgta gcatctgcat ctcttgtatc agtatttgtc atagattgta tacttatagg
     9481 attttctcct cctattttta cagttcctac acttacttct ctcgtctttc ttctctcgta
     9541 actcatattc cacctctata tattatttta tattgaatct ctttaaatat taaaatatta
     9601 taacctacat gtatattata aattaaaaag ctatctagtt aaataagaat aaatttcata
     9661 tataaaaatt aatttattct tatttaaaac tacatagcct attttcttaa aatctctaaa
     9721 aaactaaatc attgaaataa tcttagtata tctttatatg tgataaaaag cataaataac
     9781 attaaagctc caaaaccaac aacatttatc atgccttctt tatttgggtc gagtttttta
     9841 ccacctctaa cagcttctag taacagcata agtattctcc atccatcaag tgctggtatt
     9901 ggtaatagat ttactatacc taaatttaag cttatgacag ctgctaaata cactacattt
     9961 atgatgcctg tacgagcagc atctgatacc ataccaataa cacctactgg tcctgctaca
    10021 gcattgccaa tccctcctgg tacacgtcct gtaatcattt gacctagaaa tgttagcatc
    10081 tgttttgtca tatctacagt agttttacat gcatttacta ttgaccccaa aaagtctttt
    10141 tctctttgtg gttgaatccc tatttcatac tttccattgt tatttttagg tgttacattt
    10201 acaactttat cacttccatc tctagttata ctaagtttta attttccacc agaagaagta
    10261 tttattatat ttgcaacttc atcccaagtc ttaacagaat taccattaat tttatttatc
    10321 ttatctccta cttgaagccc tacagcctgt gcaggagtat ctggcattat ctttcctaat
    10381 ttagtcgttg gagaacctat atacaaaaat actgggacta aaagtattgc tgctaatatt
    10441 atattgaata ttggtcctgc tacaatagta ctaaatcttt gtaatataga cttgtttcca
    10501 aaagaatttg gacttatttg ttcttcatct tcaccttcca tgctgacata tccaccaatt
    10561 ggaagtgctc ttatagaata ctctgtatct ttttttacac tatatatctt aggccccata
    10621 cctatagaaa actcatttac tttgatacca cttctttttg caaatataaa atgtcctaac
    10681 tcatgtatta atacaattat actaaatagt attaatgcgg ctattatagt cataaattca
    10741 ccttcctatt ttttttatca gatttgcaac ataagctcta ctccatctat ctgtttctaa
    10801 gatttcttct aatgttggtt cacttatgct actatgaact tctaaagtct tctctatgta
    10861 atatggtatg tcatagaaac caattttatc ctctaaaaat tcacttacta aaacttcatt
    10921 tgctgaattt aaaacagaag aataagttcc tcccattttt aaacactcat atgccaattt
    10981 taaacatgga aaagtttcta aatctggttc ttcaaaagtt aaagtactaa attttgaaaa
    11041 attcattctt tcaaagctac tttccattct atctggatat gtaagagcat actgtattgg
    11101 tagtctcata tctggacatc ctaattgagc aattattgag ctatctgtat actgcaccat
    11161 tgaatgaatt atgctttgtg ggtgaactac tacatctata ttttcttgtt ctactccaaa
    11221 taaccatcta gcttctatca cttcaagtcc tttattcata agcgttgaag aatcaatact
    11281 tatttttctt cccatactcc aatttggatg cttcaaagct tcatttttag ttatatttgc
    11341 aagttctccc ttttcctttc ctctaaatgg accaccagat gctgtaagta ttatcttttc
    11401 tatatttttt ttattttctc catttagaca ttgaaatata gcactatgtt cactatcaac
    11461 aggaagaata tttacattgt atttttctgc ttctttcatt acaagttcac cagcagttac
    11521 caacgtttct ttattcgcta atgctatgtc tattcccttc tttatagcac aaagagttgg
    11581 gactaatcct atcataccta ctacagcagt taaaaggaca tccacttcat caagtgatga
    11641 tattattttt aatccttcca ttcctgctaa aacttctatt tctatatttt taggcaacat
    11701 ttcttttaat ttcaatgctt tattttcctc aaaaactgtt acatatttag gtttaaactc
    11761 tactatctgt tctagcaata attcaatatt actgttagct gatattgcaa ctatctcaaa
    11821 cttatctcta ttatctctaa ctacatccaa agtttgttta cctatagagc ctgtagaacc
    11881 taaaattgat atctttttca tataataatc ctttctaata ataaatatac cctgtcaaaa
    11941 cattataaca ctactaaaac aattattttt actattcata gaaatttcat atattattcc
    12001 acagattaaa attaatctat tattttttgt attttgtgga atattataaa atattctagt
    12061 attattaaat gccctttttt atttccacaa catcttctat tttacagtct agtgctaaac
    12121 atatcttctc cattgtatcc atagaaacag atttatcatt tcctagtctg cctaaaacag
    12181 aaatacttat acctgatata tttacaaatt ccattttctt tatttttttg tcaattaaca
    12241 atttccataa tggagcgtat gaaatcatgt tatcacctcg caaaatatat tctatcacat
    12301 tttatctatt ttggaaaatt ttcatctatt tagataaata attatttatc taaatagatt
    12361 tcaattatag tttattaaat ataaatatgt taaaataaaa caaaagcata agttcaaaaa
    12421 ttaaacttta tatata
//
LOCUS       DAEMIK010000131        12374 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEMIK010000131 DAEMIK010000000
VERSION     DAEMIK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.132"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            384..746
                     /locus_tag="KPK87_003001"
     CDS             384..746
                     /locus_tag="KPK87_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2539125.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            811..1428
                     /locus_tag="KPK87_003002"
     CDS             811..1428
                     /locus_tag="KPK87_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG2539126.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNTITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVLMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1425..1910
                     /locus_tag="KPK87_003003"
     CDS             1425..1910
                     /locus_tag="KPK87_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG2539127.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            1953..2471
                     /locus_tag="KPK87_003004"
     CDS             1953..2471
                     /locus_tag="KPK87_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG2539128.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2607..3566
                     /locus_tag="KPK87_003005"
     CDS             2607..3566
                     /locus_tag="KPK87_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2539129.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3592..5295
                     /locus_tag="KPK87_003006"
     CDS             3592..5295
                     /locus_tag="KPK87_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG2539130.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5556..7658
                     /locus_tag="KPK87_003007"
     CDS             5556..7658
                     /locus_tag="KPK87_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG2539131.1"
                     /translation="MRKMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCMSSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7671..8111
                     /locus_tag="KPK87_003008"
     CDS             7671..8111
                     /locus_tag="KPK87_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG2539132.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            8147..8950
                     /locus_tag="KPK87_003009"
     CDS             8147..8950
                     /locus_tag="KPK87_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG2539133.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            8990..9586
                     /locus_tag="KPK87_003010"
     CDS             8990..9586
                     /locus_tag="KPK87_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG2539134.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9692..10555
                     /locus_tag="KPK87_003011"
     CDS             9692..10555
                     /locus_tag="KPK87_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG2539135.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10622..11611
                     /locus_tag="KPK87_003012"
     CDS             10622..11611
                     /locus_tag="KPK87_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG2539136.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11703..12314
                     /locus_tag="KPK87_003013"
     CDS             11703..12314
                     /locus_tag="KPK87_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2539137.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5005 a         1213 c         2326 g         3830 t
ORIGIN      
        1 acaactatct attagatatt gagaaagtca aatcctatat gaatctacag aaatgatttt
       61 aatgaatctg aaaaattttt ttatacaaac ataaaaaagg tataaagttg ttttattata
      121 atttaattac ttttgataac tttttatgtt ttatacaatt atatttttaa taactcgtca
      181 aaacgccaaa catatattac aatagatata taagtttttt tgttgacaaa ttatgttaag
      241 agaattaagt ataatatggc ataaataatt acttttattt atcaatgaaa tgtatttatt
      301 gtgtagcgaa aaatagtcag atagtataga caataactga agataaaagc gtttattgtt
      361 gtacatatgg aggagttagt tttatggaaa ataaaatttt gatagtagat gatgctattt
      421 ttatgagaat gataattaaa gaagctctga gtaaaagtgg ctataataat ttaatagaag
      481 caaatgatgg agaagaagcg tgtaatatat ttaaatatga aaagcctgat ttgatgttgc
      541 ttgatattac aatgccaaaa aaagatggtt tagaggtgct aagagaaata aaaaagttgg
      601 attcaagtgc taaggttgta atgtgctctg caataggaca agagaatatg attgtagaag
      661 ccataaaact tggagcgtta gattttatag tgaagccctt caaaacagaa acattagtta
      721 aagttgtaga taatgcttta aaataaaagt gtaaatatac actaatacaa tgaaaagcaa
      781 ataaatgcaa taataaaggg aggggtttat attataaatt atttagaact tgatgatgtt
      841 catatagatg ctcttagaga aattggtaat ataggctctg ggaatacaat tactgcactt
      901 gcatccatga taaatagcaa tgtagaagta ttaataccta tggtcaagat acttgaatat
      961 aatgaagcga caaatttgct tggtggtcca gaaaacaaag tagtatgtat tttactcgat
     1021 atgaaaggtg atatcaatgg aatgtttatg tttttgttgg atgaatcaat aactcaactt
     1081 atgctaagtt ctctttttaa taaagaagaa gcttttttag atgagattga ggcaattgaa
     1141 atttcagcaa ttaaggaaat aggaaatatt atggcaagtt catatgtaaa tgctattgca
     1201 tctatgttaa acatgactat aagtgtttca attccagata tatgtattga tatggttgga
     1261 gctgttctaa atgtacttat gataagattt tcagatgttg gtgacaaagt cttatttata
     1321 gaaaataaat ttaaaatgag tgataattat tttactagtc acattttgat gattccagaa
     1381 atgagctcac ttagagaaat actcgtgagg ttgggattag aagtatgaat agagagatag
     1441 tagtgaacat tgcagatatg aagatagcct atagaccaaa tgttttagtt acttatgcac
     1501 ttggttcttg tgtaggagta tgtttgatag ataaagtagc tggaattggt ggaatgttac
     1561 acgttatgtt gccatatagt aaagatgcta ttaacattga aaataagtgt aaatttgctg
     1621 acacaggaat aaatgagctc attaaatcta tggagaatat aggtgcaaat agaatatata
     1681 taaaagcaaa aattgctgga ggtgctcaga tgtttttagg aagtagcaat tcagtaattg
     1741 caagtatagg acataggaat gttgaggcag taaaaaaaac tcttttagga ttaaatattc
     1801 caatattagc tgaggacaca ggtatgaatt atggtagaac aatacgtttt tacactgaga
     1861 cgggtgaatt acttgtagat tcaataaata aaggtaagaa gaaattataa tatatatttc
     1921 atagaaagtg tattttagga gggaggtatt aaatgagatt tccagatgac ccatatttag
     1981 attatggact aattgatgaa ggaattaagt atctaaagtt taaagtagat gagcaagatt
     2041 ttggggttga acttgaaaag gtaatagaaa ttactggaaa tcaagaaatt atatttattc
     2101 cagaactacc tacatattct aaaggcataa taaatctaag aggaaaaatc attccgataa
     2161 tagatatgcg tttgagactt aaaaaagatg aattaatgac tacaaattgc atgtatatag
     2221 tagtaactga aataaaggac ttggtagttg gatttgtggt tgatagagtt gatgatatag
     2281 taagcttaga aaaaagcaaa atatcacctc caccaagagt tactatagaa tattcggatt
     2341 attttgtaag tggagttgga gaattagaag gtaaaataat tactttacta gatttagaga
     2401 aacttttgac agataaagat gaagttttaa tagatgaatt aatcaatggg tttgattgca
     2461 ctatcatgta atatagggat gtagttggag tgttattata aaaagattgt gatgagaaca
     2521 tttaagaata tagtggtata attattaagt ataaaaattt taaatcatta aaatttgaat
     2581 atattatgtt taagagaggt ttgtatatga aaaataagca acagggaaca attttagtcg
     2641 ttgatgatag tgttctgatg tgtgacttaa tagataaaac acttacaaaa gataatttta
     2701 aggttaaaaa agcatttaat gcaatagagg caaaagaatt tattgaagaa tttataccag
     2761 acattatttt gcttgatata attttaccag ataactctgg ttttgagttt tgtaaggaag
     2821 taaaatctaa tgctcgtaca gcagatatac ctataatttt tataacttca aaaaatacag
     2881 atacagacat agtaaaagga tttggtgtag gagcaattga ctatatggtg aaacctttta
     2941 gtatgacaga attaaaagct cgtgttatag ctcatcttga agtaaagaaa tctcaagata
     3001 aattaaaaaa gataaataat gaactggaat cttctcttga aaaattgaat agactcgttg
     3061 taagagatta tcttacaggt ctttacaata ggcgacatat aattaatcag cttatggagc
     3121 aaagaagaat taataaatat agtgaaacat caatatccct tatccttggt gatattgatg
     3181 actttaaggt tatcaatgat acttatgggc atgaagcagg ggatttcgtg ttaacagtaa
     3241 tatcaagtat aataaaaaag aattgtagac aaattgatac tgtatcaaga tggggtggag
     3301 aagagttttt gataatgctc acaaatactc caatagaagg tgctaagata ctttcagaaa
     3361 gaatccgtaa agagataaag gagtttgatt tcaactataa agaacataaa ataaagtgca
     3421 ctattacatt aggagtagta gaagtaaact caaatatatc attagaagaa aacatatcct
     3481 tagcagataa agcaatgtat gagggtaaaa acttaggaaa agattgtagc gtagtgagta
     3541 caatgaaagg attaaaaaag atataaactt actttataaa ggagtacaga tatgaaaaga
     3601 gaagtaaagc tttctaaatt agcaattgta ttgttgatta ttatagtatt ggctgttcta
     3661 gcagggattt tttcttctat ttcatcttat aaagcatttg aaaaagtaga aaaaagtatt
     3721 aatgatatga ataatttgag gactcaaagt cagtatataa aagatacaac tagagagata
     3781 agtgatgtat taagaagata tgtatttaca ggagaaaaaa agaataaaga tatttatgaa
     3841 aaagaattat acggaaaaac aagagaatca gctttaaaca aattagaaaa aattggtctt
     3901 acaaagagcg agtcaaataa aatcttagaa gcaaaagaaa tatcagattc tttagtacca
     3961 acagaacaaa gtgcaataaa ggcagtcgaa agtaatgata agagtaaagc tcaaagtata
     4021 atttttggta gtgactatat aaactcccaa gagaaaatgg attctttaat aaatgaattt
     4081 caagaggata tagttacccg ttcaaattcc aaaattgtag aagtaaaaaa agaattacaa
     4141 agtctaatat taagaacaat atttatactt tcccttttaa taatattgac aataatagaa
     4201 tatacagtaa taaaattaaa aatagtgatt cctattaata aagtagaaaa acacttttca
     4261 agaattgcat ctggggactt aagaacaagt atagacgttg aagaaagtaa atctgaaata
     4321 ggactattag tgagttctgt caaaaagatg caaagtatgc ttttaaatta catagacctt
     4381 atagacagta cattaactag tattgcaaaa ggagatttaa gagtagagat agaccaagaa
     4441 tttataggtg attttaaatc aattaaaata tctttagaaa gtataataaa ctcttttaat
     4501 ggtgattttt ctgaaataaa tttatcagca gaacaagttg caagtgcttc agaacaagtt
     4561 gcagcaggtg cacaagctct ttcacagggt gctactgaac aagctagttc agtagaagaa
     4621 ttagcttcca cgattaatga gatatcagat ggaattaaga agtgtgcaga tagtgctagt
     4681 cttgcatgtg aggtatctga ccaatctgca tcacaagttg gttttggaag tagatgtatg
     4741 gatgatatga tgacatcaat gggtgaaata agtgaaaaat cgagtgaaat atcaaaaata
     4801 atcaaaacaa tagatgagat agcattccaa actaatattc ttgcattaaa tgcagctgtt
     4861 gaagcagcaa gagcaggtca atatggtaga ggatttgcag ttgtagcaga tgaagttagg
     4921 tctttggctg gtaaaagtgc aaaagcagct caaaatacag ctattctcat agaagaagct
     4981 attaaaacag tagaaaatgg tgtcagaatt gctaaagaga ctgcaaatgc attggacttg
     5041 gtagtaaatg gtgtagaaaa aactactaat tatattaatg gtatttcttc ggctgtacat
     5101 agtcaagaag tagcaattac acagatttta tctggaatag agcaaatttc catagtagtc
     5161 caaatgaact cagcaacagc agaggaaagt gcagcagcct cagaagagat gtctgctcaa
     5221 gctcaaattt taaaagaatt agtagaaaaa tttcatctaa aggaaagtga agtaagagaa
     5281 tcatcatggg tttaaattat gagatgaata ttgaagtaag agaattatca tgtatttaaa
     5341 ttatgagatg tatatatatt gaaataagag aattatcatg tatttaaatt atgagatgta
     5401 tatatattga agtaagagaa taattgtgta tttaaattat gaggtacata tagtgagata
     5461 agagaatcat caaggattta aattatgaga tgcatatata atagaaaatt atttgtaggt
     5521 cgtaggtgat aaatgtgaat tatattgaac cgagtatgag aaaaatgtta gatatgttta
     5581 tatttgagac aagtttatta ctcgaacaat tagatgaaat tatgattcaa atagaaaaag
     5641 agaaagtatt tactcataac aacattaatg aaacttttag aataatgcat acgataaaag
     5701 gttcagcatc tatgatgggg ttagacaata tatctgcttt agcacatagt gtagaagact
     5761 tgttttatgc tatacgtgaa ggtaaaatag aagttttaga caatgaccta ttatttgatt
     5821 tagtctttca atcattagat tttataagaa gagatataga tgagttacaa tcaagaggtt
     5881 gtatgtcttc tgattactct aattttattg aaggaattga agagtatgtg agtactttgg
     5941 aattatcaag ttctatgaaa tcaaaggata atgcaaattt agaaagtaaa gacgaaaaag
     6001 aagtaatcga ttcaaatttt gaatacaaag aagactctga tgtagtaaaa ataatctttg
     6061 aacaaggttg catgatggaa aatgtgagag ctttcatgct tgtatttgaa cttagagatt
     6121 actgtgaatt tatagatttt tatccaaaag atgtagaaac aaatgcagag tctacagagt
     6181 atattaaaag ttatgggttt tatatacaat ttgtagctaa agaggataag gcaatagttt
     6241 ataaaactat agaagataat ctctatgtaa aagaatacat aaatattagt aaaaaagaaa
     6301 tagaaatgaa aagtacatca aacaatgatg cagtagaaaa taaattaaaa gataataata
     6361 aagtaagtaa aagttatcaa aataactata cagaagatga gatacaagaa gatgttttaa
     6421 atgaaaaaac tgatatggaa aaagatagtc taagtgaaga aaatgaacat ttaccaataa
     6481 gtaagcagag tattatgagt gtaaatttag aaaaattgga cttacttcaa aatatagttg
     6541 gagaaattgt aattatggaa tcaatggttg taaatagcgt aaattctaca ggtgttgata
     6601 tagataattt caataaggca acaagacaat tacacaagct tacagatgaa ttgcaagata
     6661 tagtaatgtc tatgcgtatg attcaaattg ctggtgtatt tcaaaaaatg catagaattg
     6721 tacgagatat gaataaaaaa ttagataaaa atgtagagtt aataactata ggagcagaga
     6781 cagaggtaga taagagtgta attgataatt tagcagaacc ttttatgcac cttataagaa
     6841 atgctatgga ccatgcaata gagcctattg aggagcgtat agcgaaaggt aaagaagaag
     6901 tgggaaagat agttttatct gctgaaaatc taggcggaga agctgtgata agtatttcag
     6961 atgatggtag aggcattatg agagataaga ttttggcaaa agctaaggaa caaggtcttc
     7021 ttaaacatca aagtaatgag tatactgatg acgaggtcaa ttctttgatt atgactccag
     7081 gcttttctac aaaagaatct ataactgaat tttctggaag aggagtaggc atggacattg
     7141 ttcgtaaaag tattgaaaaa gttggaggtt caattgaagt aaaaagtaaa caggatgaag
     7201 ggacaacctt tactataaaa ataccattaa ctctttctat agtagagggt atgaagttta
     7261 aagttggaga ttcactattt actttaccaa tatcatctat aagaagaaca tttaaactta
     7321 cagacttaaa tcaaataatt acagaaggaa ataagacaga gttaattaca atatatggag
     7381 cctgtattcc cattataagg ttacataaaa tatataatat tcaaacaaaa gtaaccaatc
     7441 tgatgaatgg gaatatgata attattgaaa atgagcataa agcagtatgc atttttgttg
     7501 atgaaataat aggtaatcag caagttgttg tgaaaccatt tccagtttat tttaaccgat
     7561 ttaatataaa aaataagggt ttatcgggat gtacaatact tggggatgga agtataagtt
     7621 taattataga tgcagatagc attatagaaa tatattaggg gatttaaagt atggctaaag
     7681 atgaaattat taaacaagtg ttaacatttt atgtcaatga tgttatttat ggaatagaat
     7741 tagaaaatgt gattgaaacc ataagatttc aatcaataac gtatgtacca tgtcttccaa
     7801 catatataag tggagttatt aatttgagag gaagaataat accagttatt aatatgcata
     7861 taaagtacaa tcttcctaaa gctgattata atgagagaac ttgtataata attacaaagg
     7921 ttgatgagta tcaagttgga attattgtag ataaagttgt tgatgttatt catatagaaa
     7981 atctaaattt actagagacg actaattcta acaacactaa tattaataaa gatataaaag
     8041 agattgcgaa agttgaagat aattctatat taattttaga tattcgaaag tttttgatta
     8101 atagcatata gttagttctg ataaattagc aggaggaagt ttttgcatga taaaactcac
     8161 agatgaagaa tttatagttc ttgtaaacca tgtaaaaaaa gaatatggta ttgatttaag
     8221 taaaaaaaga gcgttaattg aaggtcgctt atacaataca atgatagaga aaaaacttag
     8281 ttcttttagt caatatatga atctattatt taaagataag acaggaaatg aagctataaa
     8341 cctaataaat agattgagta ctaaccacac attttttatg agagaacctc aacattttga
     8401 gtttatacag aatagtatac ttcctttttg ggaagaaaat aataaaataa gaaacataaa
     8461 tatatggagt gcaggatgtt ctagtggaga agaagcgtat tcaatagcta tgactttgga
     8521 tgattatttt ggatataata aagagttgtg gaacattaaa atcatagcca cagatatttc
     8581 aactaactca cttgaaaaag ctaaaagagg tatatacata gaatcaaatg ttaacaatgt
     8641 tcctgagtta tggaagaaga agtattttat agacaacaag gatggtacat ttaaaatttg
     8701 tgataagatt agaaaaagtg taatttttaa gccgtttaat ttaatgaatg atttctcata
     8761 tcaacatttt gatttgattt tttgtagaaa tgttatgatt tattttgatt taaaaacaag
     8821 agaagaactt ataaataaat tttataatgc taccaaaaaa gatagttttt tgtttgtagg
     8881 acatgcagaa gtaatcaaca gaagtagtac taaatacaaa tatgtaaaac ctgctatata
     8941 taaacgataa aaaggtattt ttgatatatg attttttgga ggcggtaatg tggaacgttt
     9001 taataatatg gttattgcta taggcgcatc tgtaggtggt acagaggcaa tacttgaaat
     9061 tataaaagac ctaccaaaat caacaccagg gattgttgta gttcaacata tgccagctat
     9121 atttacttat atgtatgcac aacggcttga caagcagtgt attatgaatg taagagaagc
     9181 taagaataat gatagagtcg aacaaggaaa tgtgttaatt gcacctgggg gttaccagat
     9241 gaaattgtgt acggataaac aaggatatta tgtaacttgt gaaaagggag aaagaataag
     9301 tggtcactgt ccatcagtag acgttttgtt tgattcagta gcagaagtag caggtaaaaa
     9361 ttcaatagga ataatactta caggtatggg ttatgatgga gcaagtggac ttttaaaaat
     9421 gaaagaaaat ggttcattta caattggaca ggatgaaaat tcttgtatag tatatggaat
     9481 gccaatggta gcttttgata gaggttctgt tatgttacaa ttacctcttg gtgaaatttc
     9541 aaactgtttg ataagacata ttaatatttt aaaacaaaaa gattaatttt ttataaataa
     9601 aaattaatct tttattaaat tatatggata aaaaacccta gaaactccaa atataataag
     9661 agagatttat atttaggagg aaaatgataa tatgaaattt tttaaagtcg atacaaaagc
     9721 taaagtttta agatatgtat ttgttttaac attgactata atggctgttt tggcaattaa
     9781 acatgactat aaatataata atttaaggaa agatgcttca attaatttaa atagtaataa
     9841 ggaagcagtg actgttatga gtaaccagtc attaccaaag ggtaatacaa atagtaaaca
     9901 aactttatca gtaagtacaa taggaagtaa aacaatagtg aaaaatgatg agtatttaga
     9961 aagtactata aacatgcctg taataacaaa ttcaaataaa attgttgaaa ggtctacaaa
    10021 tgataagata aaaaatgaca tatttgaatt ttataataaa tcatataaag aagcaaagca
    10081 gtatcttaag gataatcctg atgagaaaaa taagtttgtt gcaaatgtag actttgaact
    10141 taaaaagaat actgattctg ctcttagtat aaaagtgaga tattatactt atagtggtgg
    10201 agcacatgga ttttatcaag atatagcata taatgtggat atgagaactg gaaaattttt
    10261 agaattaatg gacttgttta aggataatac taaatataaa gaagttatag atgaagaaat
    10321 taaaagacaa atagctgaac ttgaaaaaaa ggatgaagaa aatataggaa tatataattt
    10381 taaaggtata aaagagaatc aaaactttta cttgcaagat gagaatctag ttgtatattt
    10441 tgatttatat gatataactc catatgcagc agggatacca gaattttcaa taaataaagc
    10501 gttgataagt tctatgctaa aaccagagta tgtagattta tttgatttga aatagaagat
    10561 taaattaaat gttatgctat aatatttagc atagagactt attaaaattg ggggtataaa
    10621 aatgctactt ttaaaaaaag acgacataaa aaaagtattt acaatgagag atgcaattga
    10681 agctgataag gaagctttta gaatttattg tgaaggaaag agtgtaaatc ctttgagaac
    10741 caatatatct gttccaagtc aagatgctag tatgttattt atgccaggat atgtggaaga
    10801 attaggctgt gggggaataa aaattgtatc tgtatttcct aaaaatgctc aaaaaggcaa
    10861 accagtaatt ccagcaacag tactattatt agatggagaa actggagaag ttagtgcagt
    10921 attggatggt acttatgtaa ctcagattcg tactggagca gcctctggag cagctataga
    10981 cgtattggct aaaaaagatt ctaagattgg agctttgata ggtctaggag gacaaggtga
    11041 gccacaactt gaagccatga tagaagctag aaatttggat gaggtaagag tttttagtag
    11101 aaacaaggaa agtagagaaa aatttgcaga agaaatgaac ttaaaattaa gtaaatataa
    11161 tactaaaata gttgcagttg aatcatctga tgaagccata aataacgcag atgtgatagt
    11221 tttagctact ccatcaaaac aaccagtact taatggaaac ttagtgaaaa aaggagcttt
    11281 aataagtgca gtaggttcat atatgccaga tatgcaagag ttgtgcccaa actgtttaac
    11341 tagagcgtct aagatatttt ttgaatcaac agatgcagtt ttatctgagt caggagatat
    11401 attaattcca ttaaaagaag gtaagataag taaagatgat tttagtggtg atttagggaa
    11461 tgttataaac ggaactattc ctggaagaga agatgatgat gagataattg tatttaaaac
    11521 tgttgggata ggtgtacaag atgtcgtaac agcaaaaaga atatacgata aagcaaaaga
    11581 aaacggtatt ggaatagact ggaagtggta aaatattcaa aataattcta tagttagaca
    11641 aatttatgtt ataatgttga taaataaatt ttttattatt ttaattgagg gaggatgttt
    11701 gtatgaaaat ggtaaaaaag ttaatattat ctacgattat ggctctagtt ttagttctaa
    11761 ctatagggtg ttctaatgca gaagaaaaag taaagtcgaa ttttgaagct aatttagctg
    11821 cattagaaat gtataagtat gatgaaaaag gaatttctga tgcattaaca gaagctgaat
    11881 ataaacagat tcaaacttat aacagcaagg acaaggtttc aatgattgct caattgacac
    11941 ctctttatgg taaagaaaat gctgaaaaga tatacgaaac aattgttaaa ttaattcaag
    12001 gtgttgaaaa gacagtaaca gtggaatctt ctagtaagga taagataact ttgaaagttg
    12061 taagtaaagc tttagatact agtaaagtaa tgagtgaagc gaacacttta gtaaaatctt
    12121 atgtaactga tgaaaagaat tacgataaaa aagaaaaaga agttcaagat gatatgacaa
    12181 aaatgattat agacctttac aataagaaac caactaagga agttacaact atgattacat
    12241 atactaaaaa agatggtaaa tggcaaccac ctagtaatat acaaaagtta tgtgaaggaa
    12301 tatatacaat ttaatttatt ttaaagacac aaaactatct tttaagcata taataaaatg
    12361 tttggaagat agtt
//
LOCUS       DAEMIK010000132        11819 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEMIK010000132 DAEMIK010000000
VERSION     DAEMIK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.134"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            109..1284
                     /locus_tag="KPK87_003014"
     CDS             109..1284
                     /locus_tag="KPK87_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG2539138.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            1387..2406
                     /gene="hrcA"
                     /locus_tag="KPK87_003015"
     CDS             1387..2406
                     /gene="hrcA"
                     /locus_tag="KPK87_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG2539139.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            2436..3056
                     /gene="grpE"
                     /locus_tag="KPK87_003016"
     CDS             2436..3056
                     /gene="grpE"
                     /locus_tag="KPK87_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG2539140.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            3148..4995
                     /gene="dnaK"
                     /locus_tag="KPK87_003017"
     CDS             3148..4995
                     /gene="dnaK"
                     /locus_tag="KPK87_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG2539141.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            5206..6360
                     /gene="dnaJ"
                     /locus_tag="KPK87_003018"
     CDS             5206..6360
                     /gene="dnaJ"
                     /locus_tag="KPK87_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG2539142.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            7326..8261
                     /locus_tag="KPK87_003019"
     CDS             7326..8261
                     /locus_tag="KPK87_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2539143.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            8297..9670
                     /locus_tag="KPK87_003020"
     CDS             8297..9670
                     /locus_tag="KPK87_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2539144.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(9858..10625)
                     /locus_tag="KPK87_003021"
     CDS             complement(9858..10625)
                     /locus_tag="KPK87_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539145.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(10655..11764)
                     /gene="ugpC"
                     /locus_tag="KPK87_003022"
     CDS             complement(10655..11764)
                     /gene="ugpC"
                     /locus_tag="KPK87_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG2539146.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         4597 a         1317 c         2071 g         3834 t
ORIGIN      
        1 atactgttat acaatataag tattatatat tgcttgatgt gctattttgt ttgatttata
       61 atttagtaat tgatgtttta agataattta agaaatgagg agtgaaagat gctaggactt
      121 tatgtacata ttccattttg tgtgaaaaaa tgtaagtatt gtgattttaa ttcttataaa
      181 atggacatag attcaaaaaa aagatatata gaagacttaa aaatagaaat ggaattatat
      241 agtaataaat tatataaaga taataaatat aaaaataaag aatgctgtag tctaaataaa
      301 aatgacaaaa taacgagtat atttgtaggg ggaggtacac ctagtatact aacctctgat
      361 gaaataagag aagtatttat aagtataaaa gaaatgtttg atatagatga gaatgcagaa
      421 ataactatag agtgtaatcc tgggacatta acattagaaa aattaaaaac tatgaaagag
      481 attggcataa atagacttag tatagggcta caagctattc aagaaaagca tttgaacttt
      541 ataggaagaa tacatactta tgaagaattt gagaaaaact ataaagatgc attaagtgta
      601 ggttttaaaa atattaatat agatttaatg tacagtcttc caaatcaaac tctatgtgat
      661 tggaaggaaa ctcttgaaaa agttgtacac ttaaatccaa cacatatatc agcttattcc
      721 cttatactag aagaaggaac tgaactatac aatatgtatg aaagtaataa atttgaactt
      781 atagatgaaa atgtagatat agaaatgtat gagtatacaa taaactactt aaagtcaaaa
      841 ggatataatc aatatgaaat atccaactac tcaaaagaag gctataattg tgagcataat
      901 attttatatt gggaatgtga acattatata gggattggag caggagcttc tggctatatt
      961 aatgagaaca gatacaataa tgtagaatct ttagaagatt atcatttaag tttggtaaaa
     1021 agagaaaaac caatacaaga aaatgaaatt ctttctgaga aagatatgat agaagaaaag
     1081 atatttatgg ggcttagaat gaataaaggc ataaaatttg aagattttaa aaaaaaattt
     1141 ggaatagatt ttagagagaa gtataataag caaatagaaa tgctattagc tagaaagctt
     1201 ataaatcaga gctttgaagg tatacaatta actcaaaaag gaagagaaat atctaatagt
     1261 gtatttatag aatttatgga ataattttta aggtgttgac aaaataaatt tataatggta
     1321 tataatatat ataatctttt tagcactcga acttagtgag tgctaacagc taggaggtat
     1381 aataatatgg aattaaatga aagaaaatta aatatcctca aagctatagt taaagattat
     1441 atagaaacag ctgaagctat aggttctaga acaatatcta aaagacatga tttaggtgtt
     1501 agtgctgcta ccataagaaa tgaaatggct gaccttgaag aattagggta tttaattcag
     1561 cctcatactt ctgcgggtag agttccatct gaaaaaggct ataagttata tgtaaactct
     1621 cttatgagta aaagcgaatt ggatgataat gataagattc ttattgaaca atgtatgaat
     1681 cacaatataa atcatataaa agaattgatt catgaaactt ctaagctatt atcgcaacta
     1741 acaaactata caactgtagc agttactaag agtttaataa atcaaagtgt tataaagcac
     1801 atacagttgg ttgctatgaa tgacaataat attttactta tagttgtaac agataaaggg
     1861 gacttaaaaa aagctaacct tacaactaat gtatatttag atcaatctaa gttaaatttg
     1921 atttctgata atcttacaag gaagctctta ggtaaaagta taacagattt ggatgataat
     1981 ctaattgctt ttattaaata cgaaataagt gagtattctg ggcttataga tgagctttta
     2041 aatgctttga actcaaatat gaaagaagaa gatttttctc tatcattaaa tggagctact
     2101 aacatattta gttatccaga atttaatgat gtattaaaag caaagtcatt tttaaatatg
     2161 ttggaaaaaa aggaaacaat agctgacata attaaatcaa agggaataca aaaagacaat
     2221 ctaaatataa taattggtag tgataatgat tgtgaactgg cacaggattg tagtatagtt
     2281 acagcaactt ataatgtaga tagagacttg gttgggcgaa taagctttat aggccctacc
     2341 agaatggatt atgctagaat ttactctata ataaattata tgagtttatt aattaataga
     2401 aagtaaagaa attttaaatt gcgaggtgta aagtcttgga taaaaaaaat gagcaacagg
     2461 aagtaagaga agaaaatgat acttctatta accaagaatc ggaaactcaa gtagagttag
     2521 aagaggaagt tgttaatgaa gaatgtgaaa catcaagtga aaaaactgat gaaaaagaag
     2581 ttgatgatga aaatgtaaca gatataaatt ctaagttagc agaaaaaaaa ttgcaggatg
     2641 agttggatga attaaatgat aaatatcaaa gacttcaagc cgaatatgca aactacagaa
     2701 gaagaactca acaagagaaa gagacaatag gcgtatttgc aaatgaaaaa ataataactg
     2761 aattgatacc agttatagat agtatggaaa gagccttaga tgcatgtgaa gataaggaag
     2821 atactatgta taagggtata agccttgtac ataaacaatt aatagacact ttagtaaaat
     2881 ttggagtaga agaaatagaa gcagaatcta aggaatttga ccctaatcta catctagcag
     2941 ttatgcaaga aagtgtagat ggagttgaag caaatcaaat agtaatggta cttcaaaaag
     3001 gatataagct aggaactaaa gttgtaagac cttcaatggt taaagtttct tgctaagtat
     3061 aaattaaagg ttaaattatt taacctaata taaaatatta aaataagtaa aacttaatta
     3121 tataattact cgaggaggat tttagtcatg ggaaaaataa taggaataga tttaggaaca
     3181 acaaattcat gtgttgcagt gttagaaggt ggagaagcac aaataatagc aaatagtgaa
     3241 ggtatgagaa ctactccatc agtagtagca tttacaaaag atggtgaaag aatagttgga
     3301 gaacctgcaa aaagacaagc agttacaaat gcagataaaa caataacttc tataaaaact
     3361 catatgggaa ctgattataa agtaaatata gatggaaaat catatactcc acaagaaata
     3421 tcagcaataa tattacaaaa attaaaatca gatgcagaaa gttacttagg acaaacagta
     3481 acagaagcag ttataacagt accagcttac tttacagatg ctcaaagaca agcaactaaa
     3541 gatgctggta gaatagcagg gcttgatgtt aagagaataa taaatgagcc aacagcagca
     3601 gctcttgctt atggtatgga taaattagac caagaaaaga aaatattagt atttgactta
     3661 ggtggaggaa cttttgacgt atctatactt gaaataggag atggaacttt tgaagtttta
     3721 gctacagctg gtaacaatag attaggtgga gatgactttg accaaatagt aatagattat
     3781 ttagcagagg aatttaaaaa agctgaaggt gtagatttaa gaaatgataa aatggctctt
     3841 caaagattaa aagaagcagc agaaaaggct aagaaagaat tatcatcaac aatgagttca
     3901 aacataaact tacctttcat aactgctaca gcagaaggac caaaacactt aaatatagat
     3961 ttatcaagag ctaaatttga agaattaact agaggtttag ttgaaaaaac tatggaacca
     4021 actaagacag ctttacaaga tgcaggactt tctacaggtg atatagatga tgtattatta
     4081 gtaggtggtt ctacaagaat accagcagta caagaagctg ttaagaagtt tataggaaaa
     4141 gaacctcata aaggtataaa cccagatgag tgtgttgcag caggtgcttc tatacaagct
     4201 ggagttttag caggggatgt taaagactta ttattactag atgttactcc attatctctt
     4261 ggtatagaaa ctatgggtaa tgtaatgact aagataatag aaagaaatac tacaatacca
     4321 actaagaagt ctcaaatatt ctcaactgct gctgataacc aaacagctgt tgatatacat
     4381 gttcttcaag gtgaaagaag tatggcttat gacaatacaa ctttaggaag attccaatta
     4441 acagatatac caccagcaca aagaggaata cctcaaatag aagttacttt tgatatagat
     4501 gcaaatggta tagttaatgt ttcagctaaa gatttaggta caggaaaaga gcaaaaaata
     4561 actataacat caaacactaa tttatcagaa gcagaaatag agcaaaagat aaaagaagca
     4621 gaaatgaatg cagaagctga caaacaaaag aaagaaaaaa tagaagcatt caatcaagct
     4681 gaatctacta tatatcaaac tgaaaaaact ttaaatgaac ttggagataa aataagttca
     4741 ggtgaaaaag aggatataga aaaagctata gcagacttaa aagctgtaaa agataatcaa
     4801 gatgctacag cagaagaact taagaaagct acagatgaag taatgactaa gttccaaaaa
     4861 gtatctcaag aaatgtacca aaaagcagct caagaacaac aagcggcaca aggtgcagaa
     4921 caagcacaag acaatggacc taaagatgac aatgtagtag atgctgactt taaagaagta
     4981 gatgaagata aataagctat ataatatgta acactaaatg ataattggaa catatatgat
     5041 ttgtgaaaat catatagatg ttaaagatat atggacaata tacatcataa agaatataat
     5101 atatcataga ttatataact taaagtagaa tataagaatt aatgatagct tgtagtttat
     5161 tattctacaa gctatcgttt tgtaaaatga aggtggtgtc aatttttgag tacaaaaagg
     5221 gattattatg aagtcttagg tatcagtaaa ggtgcagaag ctcaagagat aaaaaaagct
     5281 tatagaaaat tagcaatgaa gtaccatcca gatagaaatc ctggagataa agaggcggaa
     5341 gaaaaattta aagagataaa tgaagcttat gaagttctat ctgatgatac aaagagaaaa
     5401 acttatgacc agtttggaca tgatgggcta aatggtcaag gcggttttgg tggtcaagga
     5461 ggatttggcg gtcaaggctt tggtggtttt gaagatatgt tcggagatat atttggtgac
     5521 atgtttggag gaagcttcgg aggtggaaga gcaagaagaa gagggcctca acgtggtgca
     5581 gatataagac agagtgttac tatatctttt gaagaagctg catttggtaa aaagatgtct
     5641 ataaaagtaa atagaagtga agaatgtgaa gaatgtaatg gaactggagc aaaaccagga
     5701 acatcaaaga aaacttgttc aacttgtaat ggaactggtc aagttagaac tgttcaaaga
     5761 actccatttg gtaatatagc aagttcaaga ccatgtagtg cttgtaatgg aactggagaa
     5821 gtgattgaat ctccatgtag taaatgtcat ggaactggaa atacaagaaa agttaagact
     5881 atagaagtag atatacctgc tggtatagat gatggtcaaa tgataaaact ttctggtcaa
     5941 ggtgaagtag gagaaaaagg tgctccaaga ggagacttat atattgtagt taatgttaag
     6001 tcacatccac tatttactag agatggaaat gatatttatt ttgaaatgcc aattacattt
     6061 gttcaagcaa ctttaggtga tgagatagaa gtacctactt tggatggaaa agttaaatat
     6121 agtgtgccag aggggacaca aactggaact gtatttagac ttaaagagaa aggaatccct
     6181 agaattagag gtaattcaag aggagaccaa tatgttaagg ttgtagttga aattcctaaa
     6241 aaattaaacg ataagcaaaa agaactattg agagaatttg cgaaggagtg tgggtcgaac
     6301 gtccacgaaa agaagaaaac cttcggacaa aagatagaag atatgtttaa gaaaaagtag
     6361 ttaaagtagc caatatataa tcatattttt aaaatcttag ttatttataa gattgtcaaa
     6421 ggagatatta tgatttttat attggctatt tttgtataaa aaataaatgt gcataaaata
     6481 aaactgaata taaataattt tctgaaaaat acttgtccta aaatatataa tatgctataa
     6541 tataatcaaa taaaagaaag gggtgattcc agtgggaacg gatgtggcac tgccaaatga
     6601 tgaagaaaac tttattgtaa tatacaaaat aaaattagat acttttagtg ctagattaag
     6661 tactggaaaa actcatgagc tgggataatt attgtaatta cctagactca aacttttagt
     6721 agtaaaatag taatattata tagacaaggt atctaatttt taaatctttt gtatgaatta
     6781 aaatttttat aaagggtata tctgattaga atgtaattag ggaaacctag ttgtgaatta
     6841 cttttagtat atacatgtgt aaagaaatat gtatatggta ttgggtattg tgctagatgt
     6901 gtatgtaaag gaatatgtgt ctagttgggt atatgtaaag aaatatactt ataatctatg
     6961 aaaaattttt agtataaatt gctaaaacat tgagattgtt ttttatagaa agttagttta
     7021 taccaactaa aaatgtgctt aagatttgtt tgatgtttaa ttataaataa ggttgtagat
     7081 aaaaaaataa gtaaagacaa aggtttaaaa tacttgcttt aaatatagtt tgttttgaac
     7141 acttgccaaa ttaaaataat taataaaata gacatcttct catagtaaaa tatactgtga
     7201 gaggatgttt ttgctttgtt cataaacttt tatgaaaaat ttgcatagtg tcacatttta
     7261 atatactata tatataataa aatatagata aaattaaatg attcaaaaga gaaaggtgaa
     7321 aagagatgta ttatataggt gtagacatag gaggaacagg tatacaagca ggagtagttg
     7381 ataactatgg gaagataatt tttaggtcag agtgtaaaac agtaattgaa aaaggatttg
     7441 aaggaatact taatgacata aaaataatga tttataagtt gttggaagat aataaactta
     7501 ctatgagtga cataaaatct attggatttg gtgttccagg ttttataaat aaagaaggat
     7561 tagtaacttg tgtaaattta aaatggaata aaaaagcttt taataaagag ttaaaaagaa
     7621 gatttccaga tgttgaaatt catggagaaa atgatgcaac agtagcagca cttggagaag
     7681 caaagtttgg aagtatgaaa ggtgctaatg ttggagtatt atatacttta ggaactggtg
     7741 taggtggagg aatagtaatt aatcaaaaag tcttttctgg agcacatgga ttgggttcag
     7801 aaataggtca tcaaattata ggagaaaact attttaattg taattgtgga aataatggtt
     7861 gtgtagaaac tttttgttca gcaactgcaa ttataaagta ttctcaaaaa ctgatagaag
     7921 aaggagaaaa gagtaggatt ttagatttgg cagagggaaa tttagaaaat gtaaatgcaa
     7981 agatggtgtt tgatgcatat agagaaaatg atttagtggc aattaaagta ataaatagat
     8041 ttaaagagta tttagcaaaa acttttgcaa acacaataaa ttctttagac ccagaaatta
     8101 tatctatagg aggaggcatt tctaaatcta gcgatataat acttgatgga atagaaggtt
     8161 tggtaagaaa atttgtatta tacaaaacag aagatatagc tacaattaca tgtgcaactt
     8221 taggttcaga cgcaggaatt ataggagcag cttttttata atgtaaatga actgcttatt
     8281 ggaggcgtga tgatatatgg gtaaaacaaa tgctagaaga acgctttttt tgatagcaat
     8341 aggttcagga actatgctga atcctcttaa ttcttctatg atttcacttg ctttacatag
     8401 tatacaaaat gattttcata ttagttttac aactgtatct tggattgttt cagcatttta
     8461 cctagctagt gctattgcac aaccagtttc aggtaagata ggagatttaa taggtagaaa
     8521 ggtcttattt ttgagtggtt tgatgcttgt aattttatca gcatccatag taccacttgt
     8581 tcaatcattc tttatattga tttttataag agtaattcaa gcaattggaa caagtaccct
     8641 atatccatct ggagttgctc ttatacaaaa taatataaaa gaaagacaat cttctgcatt
     8701 agcagttcta accatttttg cttcaactac agcaggatta ggtccgacac ttggaggatt
     8761 attgttggac ttaggaggat ggcatgctat atttcttgta aatatacctg ttgttttggt
     8821 ttctatatgc ttagggtatt ttttattccc aaaagaagta aaagaaaaga aatctataaa
     8881 agaaacttta aaaaatgttt tgtcaagatt agatattatt ggcatacttc tattttcaat
     8941 tgcaatgatt tttatattac tatttttgct atctatgaag gagtctttta acttagaaca
     9001 acttgtattt ggtataatac tgatgtgtct ttttatttgg catgaattga gaacaaaatt
     9061 gccatttata gatattaagc tttttgtaag taatcctaaa ttgtcaaaag tttacttgca
     9121 atttattata ttaaatttct ttaattatat tttgtttttt ggtcttccta gttattttca
     9181 agatgcctta cattatagtg caaagtcgac tggattattt atgctattta tgtctggtat
     9241 aggtatattt atatctccat taactggaaa atggatagat aaatctggaa ctagatttcc
     9301 tcttgttaca tcatcaatct ttatgtttat tagtgcagta cttctttcag tagtttttat
     9361 ccatatacct gtaataggaa agggtattat attatcatta gcaggtatta gttatggagt
     9421 tggtaatgta gccctgcaat cctctatgtt tgaagaaagt cctcgtgaca gtatagggac
     9481 agcttctggt ttgtttcaaa ctagtcgtta tttaggttct attttagcaa ctgttttact
     9541 tggaatggta tttgaagtaa atataacatc agaacaattt cagatattag gttatgttat
     9601 ggtggtctta ggtacaataa gttttttatt aaattttata ttaaagaaag aattaagaaa
     9661 agtagaatag tggatttatg gatttaataa gtctaataac tgataagttt aaaaatattg
     9721 tttgtaaaac ataactggtt tgatatttat taattctaag ttttgaagca tagagtactc
     9781 gataagtata caatattttg aattaaaatt aagcagaaat ttgaactagt ctttcaaatc
     9841 tctgcttaat ttagtagtta aattttttta tagagaaaat aagatttgac acttagataa
     9901 aaaatcattg cattttcaaa attctttaaa tcaaacccaa gtatctcatt aatcttttcc
     9961 aatctatata ctagcgtgtt tctatgtaaa aacatactag tagatgtctt agaaatattt
    10021 aaatcattat ttataaattt tattcctgta tatagaagtt cttcgtcaat agatttaatc
    10081 ttattaaaat ctattaattt attgagtata ttatcctcat taattaaatc taaagtttta
    10141 tatctaataa ggtcaaacaa atttaaaaac ttattttcag cgttgtcttt tataatatga
    10201 ggtagtaaat cagaacataa ctgtaatcta aaatcaaaat cagctttact atctaattca
    10261 ccaagataaa aaatataagt actttgataa gtttcagttt caattatatt ttgtgtagtt
    10321 acattaatat ctaagttata tttagaattt actataaaaa aaccattgtg ttgtattata
    10381 tttatatctt caaataaatt ataaaaaata ttttttagat attcaaagtc tatatgagat
    10441 tggtaaatta aataaaagtt gccttttaaa ttatcaagat tatttttatc tagaaaatca
    10501 ataacataaa tagcattatt gaaagtaact aaatggctac tagcatctaa cttttggttt
    10561 tcatatggat agatatttat ttttttatta agctgtcttt ctaaaaaatc ttttaactct
    10621 tccataaagt ctccttaaaa ataagattat attcttatct tatagctaaa gtagtttctt
    10681 tgtcaaatat gtgtactcta ctattgtcaa ctgaaagtgt taagtcatca ccaacattaa
    10741 ttctgttttt actgtcaaat ctaattgtta tcgtattttc tcctagtttt aagtgagcat
    10801 aagtttctgc tcccataagt tcttttattt caattcttga tttaaaacaa gtatctggat
    10861 tagatttaat aaaagtatct tctacatgaa tatcttctgg tctgatacct ataacaactt
    10921 cattattaat ataattgtct tttattaaat catagtgttt ttctttattc aatttaagct
    10981 taatatgttc attttgtgca taaatttcat cattttcttc tattaatatt acatcaataa
    11041 aattcatctg tggtgctcca ataaatcctg caacaaatat attagctgga taatcgtata
    11101 catcttgtgg agttgcaatc tgctgaacta ctccatcctt cattactacg attctatcag
    11161 ccatagtcat agcttcaact tggtcgtgtg ttacatagat aaatgtagtt ccaagctgtt
    11221 ggtgtagctt agttatctct gttctcatag cagttctaag ttttgcatct aagttggata
    11281 gaggttcatc cattaagaat accttaggat ttctaactat tgcacgtcct aaggcaaccc
    11341 tttgtctttg tcctccagat aatgttttag gttttttatt taaaagaggt agtaagtcta
    11401 atattttagc agcttcttta acttttttgt ctatttcatc tttaggcaat tttctaagtt
    11461 taagtgcaaa tgccatattt tcgtaaacag ataagtgggg atataaagca tagttttgaa
    11521 aaaccatagc tatatctcta tcttttggtt ctatattatt gacaagttta tctccaatgt
    11581 atagttctcc ttcagagatt tcttcaagac cagcaatcat tctaagtgta gtcgatttac
    11641 cacaaccaga tggtcctact aaaacgataa attctttatc tttaatgtct atatttatat
    11701 tttttactgc gttaaatcca tttgagtaaa gttttgatat attttttaaa attacttctg
    11761 acataatatc ctccttgata tttaaatttt aattatatag tatcaaattt ttttattat
//
LOCUS       DAEMIK010000133        11772 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEMIK010000133 DAEMIK010000000
VERSION     DAEMIK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.135"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            195..1373
                     /locus_tag="KPK87_003023"
     CDS             195..1373
                     /locus_tag="KPK87_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2539147.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLIFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYRLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHHRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVPSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1723..2604
                     /locus_tag="KPK87_003024"
     CDS             1723..2604
                     /locus_tag="KPK87_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2539148.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2813..2962
                     /locus_tag="KPK87_003025"
     CDS             2813..2962
                     /locus_tag="KPK87_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2539149.1"
                     /translation="MTTVAEGIETSFQKYFLQEIDCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(3222..5789)
                     /locus_tag="KPK87_003026"
     CDS             complement(3222..5789)
                     /locus_tag="KPK87_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG2539150.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEEVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNCASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(5792..6397)
                     /locus_tag="KPK87_003027"
     CDS             complement(5792..6397)
                     /locus_tag="KPK87_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2539151.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRSVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6771..8231)
                     /locus_tag="KPK87_003028"
     CDS             complement(6771..8231)
                     /locus_tag="KPK87_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG2539152.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGALNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8569..9147
                     /locus_tag="KPK87_003029"
     CDS             8569..9147
                     /locus_tag="KPK87_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2539153.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9438..10688
                     /locus_tag="KPK87_003030"
     CDS             9438..10688
                     /locus_tag="KPK87_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2539154.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHIFFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10849..11418
                     /locus_tag="KPK87_003031"
     CDS             10849..11418
                     /locus_tag="KPK87_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2539155.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         4066 a         1935 c         1649 g         4122 t
ORIGIN      
        1 cacatagtta tcatacatac tgtatattaa gatataatac tttttaaatt ttatataaca
       61 cagacacatg caataaaaaa gaaggtatcc ataataagct ctttgcatgt acctaaataa
      121 aaactgtaaa atagaatata taaaatctaa tctattttaa gtaaatacaa tttcacttta
      181 aaagaggatg tactatgaaa taccatatat ttttaatcat aactactata ttaataatcg
      241 aactatgtaa catttacata tgcaaaatag catcttttgg aaacaaaaat ataactatac
      301 ataagaatta caaatatatt tttttaataa aaccattaat cttcttgata ataatattca
      361 caaaattacc attaccacta tattatatat tgttttacat agtaagtcat aaattgctct
      421 taagcagaaa aagttctact aacaaccatt ttattcccaa tatggcagta ataagttttt
      481 gtattttaca ccttattact gtaagtacaa tatcacttgt taaaaataca agtccagata
      541 taatatttgc aaatatgacc tataggttaa ttagtatttt aatagcttct attttagatt
      601 ttatcttatg gtactctatg aataaaataa aagagcatat ttatataata actcaaaatc
      661 aaatagagtt cagtctattt ttaaaattta tctggttcac tttaggatat atattatttg
      721 atagtataca aattacattt gctccaccaa gttctctaac ttcctacttt atattgggaa
      781 gtaacatatt attaatctta caactaatta tattcatcaa tcatatatat tccattgaaa
      841 aaaatattta tttagaaaaa gaacatcata gactaaaaat gataaaacaa gaacaattac
      901 taaaagtaaa tttattaaag aaaaaagcta acattgatga attaacatca gcatataatc
      961 gaaaatttgc ttttgaaaaa atacagcatt acattgataa caatttatta ttttcattga
     1021 tatatatcga tttggataaa ttaaagacga taaatgatac atatggacat ttagctgggg
     1081 ataactatat tattaacttc tcaagtatag ttattgaaaa cttaggaata gatgacatat
     1141 ttgctagaat aggaggagat gagtttatag ttattatgcc taaatctaat gaaaacacca
     1201 caaaagaatt tatagagaaa ataagtaaaa ttatggataa tacgatatct tttagctatg
     1261 gtattgtaga agttcctagc gaaaacaaaa tgactgtaga agaaattatt aatattgctg
     1321 atattagaat gtacactaat aaacaaaata atcataaaag gaacaataac taaaatgaat
     1381 atttacatga gcttacttgt aatcttacta tgccttttat cttattctat ctatcattat
     1441 aattttttta aatatctgga cataatgatt aataactcta aaaataaata caaagtatat
     1501 attgtatatt ttctttttaa ttatttaatt tttattattt gtacttattt aaggttacat
     1561 ctagttataa attggacaat ttttatgtta attttattta tgcaaattaa agtaacttac
     1621 aaactttcca caataaaaaa ccttttttat tcgttaaact gctccatcat tggtttagca
     1681 ataaatatat tttttaggtc tttaatgtca atagtgttaa atatgccagc atatttattt
     1741 gatagtaatt ctattacaaa tatttacaga gtattttcta tattgcttag ttttatagtt
     1801 acaggattat atttcagtta taaaaatcat aaaaataata agattaactt tgaggcagtt
     1861 ttagaggaca aaaaaaactt aattttctta ttgagcctta tgtttggaat gtacttgtat
     1921 ctatgtttga gtttgtcaat atactatact gttggaaatg tattgatttt aaaattatat
     1981 ggaatcaagt ctgccataat agttttggta ggtaactata ttgcagaaag atatagcttt
     2041 agaattactc aaataaaagg ttatgatatt aaaaatcaaa tagaatatgt agaaagtgta
     2101 aagacagagt atgaaattaa taaattgaat gatattgtat atacagattt actgacaggc
     2161 ttctacaata gaccttttgt aaatcaaaaa ctatcagagt ggacatcaaa tcactacaaa
     2221 tttaccttat gttttactga tttaaatagc ttaaaacctg taaatgataa atttggacac
     2281 caatttggtg ataaatacat atccactact gctcaaataa taaaaaaact tataaatgta
     2341 gaaaattcct tactatttag atatggtgga gatgaatttt tattcctttt acagaataca
     2401 agttgtgtag aagcagaaaa aataatgatt aatataaatt atgaattatc aaaattaagt
     2461 aatactgacg agtatcctta tgctctttcc ataagctatg gattagttga gtgggatgga
     2521 atatccgata ttgaaacttt aatatcaaag gcagattctt tgatgtatga aaataagctt
     2581 aaaaataaat ctactttaaa ctagctagtt aagatattct acaaaatata tgttataaga
     2641 gagtactaag aatgtcaaaa tatatagatt ttgcaaatat tgaatacagt ttactaatga
     2701 atgcactgga tgtaagtgtt agtaaacacc tattagatga acactttact gttgtttggg
     2761 ctaatgaccg ttattatgaa atgttcggct atacctagct aaaaagttaa aaatgaccac
     2821 tgtagccgag ggaatagaga ccagttttca aaaatatttc ttgcaagaaa tagactgtga
     2881 tatgttacaa ggatacgtgt tctctagacc tttaccaata aaagactttg aaaaacttat
     2941 gtttcaaaac tcctccaatt aacaatataa aattgcttat tataagtgat attattgtat
     3001 caacaaataa aggaattgtc tcaaaattga ttttttctaa tcaaattttt attttgagac
     3061 attctcattt ttttgcataa gaaaataccc accaagctac ggtttttctt accgctgttc
     3121 ggtgggtatt agtagtatta tgaaatctga aaactttaag tattactact tcctctagcg
     3181 caatcacaaa ggtcgaaagc ttcaaagcga aaataggctt tttactcatc aaacgtcacc
     3241 gtacctgcca acccgtccac cgttatcatt tgtccgctct taatagctcg tgtggcaatg
     3301 tgtgtagcca taacggctgg gatgccatat tcacgtgcga caatgcttga atggctaaga
     3361 ggaccaccaa tgtccgttac aatcgcactt gcagatgaaa ataatggtgt ccatgctgga
     3421 gttgtggtaa ctgcaaccaa aacacttcca ggttgaaagc tttcaaaatc tgctggacta
     3481 ttcagtacac aagctggagc cgtcacaaca ccagtactgg taccaatgcc cgtcaacaca
     3541 attttcccat ctttctgctt ctgttgtgct tgtgatatag tttttttgtt tttctctggc
     3601 aacttggatg gtgacacata acccatgtat tttttaagtt cagcttttcg aacaggtatt
     3661 atcccgttca tatcagatag agacttgttt ttatccaact gctctataag tacctccaat
     3721 tccgattttg tgagccaata aatatcatct aaatgtgaaa tagctcctcc tcgtatgaat
     3781 cgttctgaga tttcctgaaa catacaacga atcagaggat gtcccattcc catcaaataa
     3841 atagcatttt cacgcatagg agaggtttcc tgtgcccaat ggagcaactt aaaaaatagt
     3901 tttttacgca atccacctat gtgttgtaaa atctcttctt ctgcatgttt tctacgcttt
     3961 tcaaacgtgg tctggcgtaa aaaaggactt tccccttttc cttccacaaa tgtttttacg
     4021 gattcaaaag ttggtgtaag tgtttcctgc ggtgtggaat gcgcaaaatc aaactcataa
     4081 gcggtacgac caaactcttt aaagtactta ttgattcgat ttttccattc tatccatact
     4141 tcctgtgaaa ctccaacagg cacaactgaa gacatgaaat cttctgctat ctttattgca
     4201 ggactgtttt gcagataaag gttgatgctg ttattttgct ttacccattc tgaaataccc
     4261 cacaactcct tttccgattg cagaacaatc gtatcaaacc caagcaggaa aacggaagta
     4321 tccgttatac cagcacgtcg aactgctcct tgaaataatt ttgtaaataa tgtttcactg
     4381 atagaagcag caggcaatgt aagctgaatc cttgtgaaat aaatacatgc ggcataaaat
     4441 attgtctgaa taccctccat aatttgatgg gcgttcatag tatgcagggg cttttcttcc
     4501 cattgtttaa tagcatcctc atatcctttt cgtgccgttt cccagcgtgc aacgctgttc
     4561 ataagtgtcc tacgtaaaga gcgtggggaa agggatttga tagcaatcaa aagtggcttt
     4621 gatttagcag aaagataaac atatccatta ataaccgtat atgccccgtt ttttggtaat
     4681 aattttttcg cacttttacc aaacatatct atccataaaa gctctgatgc acagtttacg
     4741 atttcaagac caagcgtggc gaacaaaggt gtaacaggat ttggcaagtg ttctgccaaa
     4801 cttccctttg tgtatacaac atcctgttct ggcaataccc attctggtgg cagaacagta
     4861 attggacgag cctgaacaat gtacaattta tctttttcca gtgcccactc tacatccata
     4921 ggcatttgat aatacttctc aattttcttt ccaagctgta tcagttgcat tacctgatta
     4981 cgagtaagag catatttttt tctcaacctt tcaggaacca tggtttcttc tgttccttct
     5041 gaagtacgaa ccgtcataat ctctttattt gccacttcat acgatataat tcgttcggag
     5101 tccttatcca ctacaattgt atcgggtgta actaagctag aaaccaccgc ttcaccaagc
     5161 ccccatgcag cgttaataat catttcgcta cgtctaccat tgattgggtt taacgtaaac
     5221 ataataccag atgaatcgga aaatgcaagc ttttgtacta caacagcaag tgcgacaatc
     5281 tcgtgcttta tattattctt cacacgataa gcaatagcac gagctgtcca taaggaagcc
     5341 cagcatctct ttacagcgtc aataacttta tcttcacctt gtatgttaag ataagtttcc
     5401 tgctgacctg caaaagaagc gtcaggtaaa tcttcagcag tcgcagacga acggacagcc
     5461 accgatatgt tccctaatcc ggcataagcc gttttaattg catccgacac ttcctgtggc
     5521 atttctccat tatggaaaag ctcccctatt ttcttagata cctcttcaag ttggctggta
     5581 ttattggaat caataccatc tagcagttta ttaattcgag actgaatatg gttcgtctca
     5641 acaaaaatct ggtatgaagc agtcgtaaca tgaaaaccgt ctggcacagg gatgcctgct
     5701 gtcaatagtt ttgataaaga catccctttt ccaccaacaa tttcaatggt tgcttgttta
     5761 tgttccagtg gcaagacata ataaatcata atcattcacc tccatcattc ataattccgt
     5821 gcagaaatat attcattgtt tcatccaaca ttttttccga actatccgat ggtcgtgacg
     5881 tgtagctgat tgataacatg gagttaatta acaaacgtgt cacaagagcg gtatccgttt
     5941 tgcgaaagat gcctgtggtc acaccattat caattaccga ttttataata tcttgatatg
     6001 cataacgagc atctttaagt ctctttcgat actcttcttc taaataatca gattctgtat
     6061 ttaaccacat cagtacactt ctatactgct ctggaaatct aagatgatta agcatgattt
     6121 ttctaaggca ctgttgtggt gaaggttcac catcaataat cctatgaact tgttggatag
     6181 ttttttcaat ttctttctca actgcatata caacaatctc atctttcgtt ttgaaaaaat
     6241 cgtatagact tgactttccc atgttggcta aaactgcaat ttctctcatt gatgtttttt
     6301 ggaatccatt ttcagccatg agactcaaag caacagcaac tattttttct cggcgttctg
     6361 cttgttgttc gggtgtaagc acaattcctt ttggcatagg gcgatacctc cttttagcga
     6421 ccactggtcg cttctttttt atttatcata cagcgaccaa cggtcacttg tcaaggttaa
     6481 tttttttcat tgtatatggt actcgagata aaaatgctca ttttagaaat tgctctattt
     6541 aagtagaaat aggcttcatc attagaaaac aagactataa atttgagata tttatcatgt
     6601 tcaaatttca tcaatatcaa agtttaagat attaataaaa aaagaattac taagaaataa
     6661 catataaaga ttcaaaaaag gagttgaact taaaattcaa ttacaaaaaa tcaaatttaa
     6721 gacaactcct ttattcttta tctctactat tctttatctc tatttattta ttacaactcc
     6781 ctaacattta ccactactgc cttaggtcta gtcatagcct cgattgaata cttaacacct
     6841 tgagttccca taccagatgc ctttacacct aaaaatggga agtggtctgg acctctttca
     6901 gtcttattat ttatttgaac agtaccaact tcaagtttat cagctatata aaatgcctta
     6961 tcaatatcac ttgtaaatac tgatgattga agcccatact ctgattgatt agcaatttca
     7021 attgcttgat ttatatcttt aactcttata attggaagta ctggaccaaa tggttcttcc
     7081 caagcaattt tcatatcaac atttacatta tctaacaatg ttggatagaa taaattattt
     7141 tttctaacat tgcctgttat taactttgct cctttatgta atgcatcatc aaccaaccct
     7201 tgaacaaaat cagttgcttt attatctata agaggagtga taactacttc atccattgga
     7261 tttccaattg tcaacttctc taccaatggc tttatttttc cgactaattt atccgccaca
     7321 ctctcttgta ctaaaattct ctttactgcc gtacatcttt gtcctgaata tgaatacgct
     7381 ccagatacta tattcttagc tgcaaaatct aagtctgcat cttctaatac tatagccgca
     7441 tcttttccac caagctccaa taataatggt accattccag atattttaga tatatgctga
     7501 ccaacttcag tactaccagt aaagttaata aaattaattc ctttatgcgt tacaacataa
     7561 tctcctatct cactacccct acctgtaata gtattaagtg ctccatctgg tatcccagct
     7621 tttctcataa tttctaccac atgaagtgcg ctaatagccc cttgtgtagc aggttttaat
     7681 acgactgcat ttccaccaat aagtgctgga gcaatctttg acattgataa atttacagga
     7741 taattaaaag gagatatagc taaaacagta cctaatggaa ctcttgaaac ataagacatc
     7801 ttatttcttg ttccaccagg gaaattatct ccactaatgg cttctccctc catatttttt
     7861 cccacatcag ctgtgtatct taaaaaatct gctgttctct caacttctgc ccttgcagat
     7921 tttatgtctt tggctatttc aagtaccagt atattagcta tctcatctat attttctaac
     7981 aataaatcag cagccttatg aaatatattg gctctcttgt atacaggcat ttcagcccat
     8041 acttttatac tctcttttgt gttttttata acctcatcta cctcatgttt tgtcatagat
     8101 tgtactttcc caacaagaga attatctatt ggtgagtaaa tttctattgg ctttctcgaa
     8161 ttagattcta cccattttcc atttattaaa tttttatata cattttcttt tgttttaagt
     8221 tcattaaaca tatttttatt cctctctctg atataacttt gtgtatttaa attatagcca
     8281 tttattcgtt atgtaaacat ttcttttttt cataaacgtt tacctataaa aaaattttaa
     8341 gttaacgttt ttctacattc ttctatttta ataacatact cgctccaaat ctatataggt
     8401 cacactttaa tatcaatata ccaatatacc aatataccaa tataccaata taaatattat
     8461 aaacataaat cacttgacaa atttataagt agacaatatt atatatctat gacttaaaat
     8521 atttttaaag tcatagatat catatataga tagaaaggat tgataaaaat ggctaaaagt
     8581 tttacagaaa tagaaaaaga caatataaga aaaagactaa tttcagaatg tgaaatcaac
     8641 tggtctaaat atggatacaa aaaaactaat attgatgaac tttgttcaaa atcaggaatt
     8701 gcaaagggtt cattctacac attctttgat tcaaaagaaa agttattttt tgaagtatta
     8761 aatggaatcc aaaacagact tatatcatca gtagatgaaa ttttaagtaa catgcctcca
     8821 aaagaaggct ttgcaaaatc cttaaaaatg ttatatcgat tatatgataa aaatccattt
     8881 ttatattcac caaataatcc tgatttcatc agcctaataa ataaactacc tcaagatatg
     8941 tttttacaac ttgagtccaa taacatacaa agtttttacg acttaattga gagacacaat
     9001 ctcaagttga agatagaaaa agaaaaagtt tttggtgttt gtaatgctct actatcaagt
     9061 ctgttggtaa aagaaaatat tgaatataac tatattgaag tgtttgactt tatggtggat
     9121 aatcttgtag aacacatact tgaataacaa tttaaaataa tttttctaag tattttttta
     9181 tactaaatag gattaatatg attgaattaa tgcaaaaaat aaacaatata atttataaaa
     9241 taaatgataa actaaatatc acacaaaact tatagaccct agagcaaacc cattccactc
     9301 tactctaagc catctataat agtttgtata tttctaataa tattagatat taaagtaaga
     9361 aaaaaattcc ttataataat tgctattata tcaattattg taaatgtttt aataatctta
     9421 gataggtagg tacatatatg gtaaaatcct cgaacacttt taataaaaac ttcaacttga
     9481 tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt gctttatctt
     9541 tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta gcagtatctt
     9601 caatcccaat tatagtattc tcccctattg gtggagctat tgcagatata ttcaacagaa
     9661 gaaacttaat ggttattttt gatttttcaa gtagtcttac agtactaatc ttagcagtat
     9721 tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc atattatcag
     9781 tcataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta gttgataatg
     9841 agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca aatattgcag
     9901 gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt atagttatca
     9961 gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt ccttttatca
    10021 aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag gatggtattg
    10081 ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct atagcaataa
    10141 acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa atcactatgg
    10201 gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt tcactggtag
    10261 ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg catattttct
    10321 ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca tttatattaa
    10381 acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta atatttttta
    10441 caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact ccaaatgaac
    10501 tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg ccccttggtc
    10561 aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc attatactag
    10621 gagttggatt tattacatta cttatagcat ttgtatcaaa gataacattg aggaacgaaa
    10681 atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt ctgaatttaa
    10741 atgcaaaatt gtgtatcagt atgttgacgt tttagaaatt gattgatata atcaatggaa
    10801 aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat gagttacatg
    10861 ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga atttcatata
    10921 agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta cgaccctgaa
    10981 agagtagaat tttgggaaat agttggactt ttacatgatt tagattttga aatgtatcct
    11041 gatgaacatt gcgttaaaca aagagaatta atgacagagc ttgatttaga taaatcaata
    11101 atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa accagagcaa
    11161 tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg tgctgctgca
    11221 ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa gaagaaattt
    11281 aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg tgcagaaatg
    11341 cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag aagcttgatt
    11401 ccagagatgg caatatagct cctccataca tataccatag aagttacatc tttaaagagc
    11461 atttctcttt ttgatgcaac ttctttttat attttgtaga ataaaaagtt taatatttta
    11521 aactcactat ataatctata ataaatacta taaataatat aatgtataat agttataaac
    11581 tttctaacct taaaagtttc taataatcaa taaacaattc taatatacaa atgtcattat
    11641 atgatttaat tatatatctt caatttatat attaaaataa atatatatat attaaattct
    11701 tatacaccaa atttttacat taaacttcta tgcaccacat ttatacatat taaattctta
    11761 tatattaaat tt
//
LOCUS       DAEMIK010000134        11715 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEMIK010000134 DAEMIK010000000
VERSION     DAEMIK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.136"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      216..320
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            530..3004
                     /gene="gcvPA"
                     /locus_tag="KPK87_003032"
     CDS             530..3004
                     /gene="gcvPA"
                     /locus_tag="KPK87_003032"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG2539156.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSIIVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            3004..4461
                     /gene="gcvPB"
                     /locus_tag="KPK87_003033"
     CDS             3004..4461
                     /gene="gcvPB"
                     /locus_tag="KPK87_003033"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG2539157.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            4884..7241
                     /locus_tag="KPK87_003034"
     CDS             4884..7241
                     /locus_tag="KPK87_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG2539158.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(7327..7644)
                     /locus_tag="KPK87_003035"
     CDS             complement(7327..7644)
                     /locus_tag="KPK87_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG2539159.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(8228..9499)
                     /locus_tag="KPK87_003036"
     CDS             complement(8228..9499)
                     /locus_tag="KPK87_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2539160.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKESSMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTLDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   10146..11497
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10146..10174
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         4511 a         1320 c         1900 g         3984 t
ORIGIN      
        1 tttggttctt tgtgtgttat tttaataata aattaagtga aaatgttatt ttacattaat
       61 atataatatc tttgcataag agtgaataac ataaaagaga aataattgat aataaaaact
      121 taacaaaaaa attaatacat aaaaaaaaat aaatataatg tgttgacatt taattgaaat
      181 attcatacaa taaaattgaa aataaatatt tccaaatgag attagcgaga gagtccttta
      241 tacgaaaagg cgccgaagaa gaaatttaag aaatgccaat ttttagagaa gctttcaggc
      301 aaaaatatcg ctaattgatg ggactctgga gagacttaag ttaagatact ataagtcacc
      361 gaagaagcaa aataatatta aattattaaa tctttcaggt atacaaacag ggatatatag
      421 gctaggatta ttttccatgc tgatatatct tttttttatg aaaaagtaca ggaaataata
      481 tcaaagttac ttaaatacag taggtctaat tttctaggga gggttatata tgaatgaatt
      541 gaaaagagta tctttgtaca atatacacaa agagcttgga gcaaaacttg ttgaatttgc
      601 tggttgggaa atgcctttag aatatgaggg tataaataaa gagcatgaga aggtcagaaa
      661 aagtgcaggt atatttgatg tatctcatat gggagaggta cagataaagg gagcagagtc
      721 agaaaaattt atacaaaacc tagttacaaa tgatataagc acattaaaga taaatgacat
      781 tatatataca cctatgtgtt atgaaaatgg tggagtagtt gatgatttat taatatataa
      841 atttggtgaa gaagattatt tattagtaat aaatgcaggt aatatagata aagatgtagc
      901 atggataata aaacaaagtg aaggatacaa tgtagatata aaaaatatat ctagtgaagt
      961 gagtcaatta gcaattcaag gacctaaagc agaagaaatt cttcaaaaaa tcacagatat
     1021 agacttaaac tcaataaaat tttataagtc aattccatca ataatagtat gtggttgtcc
     1081 ttgtttagtt tcaagaacag gatatactgg ggaagatggg tttgagatat actgtaaaaa
     1141 taaatatgta gaaataattt ggaatgaagt tctaaaggtt ggaggagaag atatatgtcc
     1201 tgctggtcta ggttgtaggg atactttgag atttgaagca gctctacctc tatatggaca
     1261 tgaaataaat gaacacatat ctccaattga aggtggctta tctatctttg tgaaaacaaa
     1321 taaagaatca tttattggca aatcaatttt atcaaaagaa aaagaaagtg gagctaaaag
     1381 aaagttagta ggatttgaaa tgcaaggtaa aggaatgcct agaaatggat atgatataag
     1441 gattggtgat aaaacagttg gatttgtaac aacaggatgt gcatctccaa cgacaggaaa
     1501 aattttaggt atgggaatta tagattctga atatgcaaaa gtaggaaatg aaataggtat
     1561 agcaatcaga aaaaaagttg ttcctgctgt gatagttaag aaaccttttt ataaaaaaca
     1621 atataaaaaa gataatatta tattaaacaa agaaaataaa ttttcatata tacctgctac
     1681 aagtgaagat aaatcaaaaa tgttaaaagt agttggatta aattcggttg atgaattatt
     1741 ttcagatata ccagaagaag taaaattaaa aagagattta aatttggaaa ttggaaaatc
     1801 tgaattagag gtaagcaaaa tagtaaaaag attgtcagaa gaaaatttaa gtttagaaga
     1861 cttgacttgt tttcttggtg ctggtgcata tgaccattat ataccatcaa ttataaaaca
     1921 tataacttca agatctgaat tttatacagc atatactcca tatcaagctg aaataagtca
     1981 gggaacttta caagtagttt ttgaatttca atctatgatt gcagaaatta caggtatgga
     2041 aattgcaaat gcatcaatgt atgatggagc aactgctgca atagaagcat gtataatggc
     2101 aatgaatcag actagaaaaa gtaaaatagt agtgtcaaaa actattcatc ctgaaacttt
     2161 gtcagtatta agaacttact tacaatataa agattgtgag atagtagaaa ttgacttttg
     2221 taatgaatat gggactacag atatagaaaa attaaaagct tctgtagaca aagatacagc
     2281 atgtgttctt atacaaactc ctaatttctt tggaattatt gaagaaatgg aagaaataga
     2341 aaagataact catgaaaata aggctatgtt aattatgagt gtagacccaa tatcattagg
     2401 agttttaaaa acaccaggtg agataggtgc agatattgtt gttggagaag ctcaatcact
     2461 tggaaaccct cttaattttg gaggtcctta tgtaggattt ttagctagca aatctaaata
     2521 tactagaaaa atgccaggaa gaatagttgg gcaaagttta gatgtagaag gtaaaattgc
     2581 atatgtatta actttacaaa ctagagaaca gcatgtaaga agagaaaaag caacttctaa
     2641 tatatgttca aatcaagctt taaatgctct tgtagcttca atatatatgg ctactatggg
     2701 taaagaagga tttaaagagg ttggtatgca gtctatgaaa aaagcacatt atacttacaa
     2761 taagcttgta caaactggaa aatataagcc aatatttaaa ggtaagtttt tcaaagaatt
     2821 tgctgtacaa ggaaatctta atatagaaac tataaatgat aaactcttag aagaaaatat
     2881 attaggaggt tataatttag aatacaatta tccagaatta aaaaattcaa ctttgctttg
     2941 tgtgactgag aaaagaagca aagaggaaat agataagtta gttggaataa tggagggatt
     3001 ataatgaaag aatacaatag tttgttgata gatatatcta aaaaaggtag gaaagcatac
     3061 tcacttccaa agttagatat agatgatata aaaatagaag atatgattga ccaagatatg
     3121 gcaagacaaa gtgagttgaa tttgccagaa gttggtgaat tagaattagt tagacattat
     3181 acattattat caaataaaaa ctttggagtt gatacaggtt tttatccttt gggttcttgt
     3241 actatgaaat ataatcctaa aataaatgaa gatatggcag cacttccaaa ctttactgga
     3301 atgcatccat accaatcttc ggatacagct caaggttctc tttctttgat gtatgattta
     3361 tcaagaagac ttgcagaaat tacaggtatg gatgaagtta cactacaacc ttcagcaggt
     3421 tctcatggtg agtttacagg gcttatgatt ataaaagcat atcatgaaaa tagaggtgac
     3481 cataagagaa ccaaagtgat aattccagat tctgcacatg gaacaaatcc agcaagtgca
     3541 gctatggcaa actttgatgt gattcaaata gcatctgata aaaatggagc tgtagacata
     3601 aatgcattaa aagaggtttt gaatgatgaa gtagcagctc ttatgcttac taatccaagt
     3661 actcttggtt tatttgaaaa aaatataaaa gaaatagcta ctcttgtaca tgaagcagga
     3721 ggacttttgt actatgatgg tgcaaacatg aacgctatta tggggataac aagacctgga
     3781 gatatggggt ttgatgtggt tcatcttaat cttcataaga ctttttcaac tcctcatgga
     3841 ggtggaggtc caggagctgg tcctgttgga gttaaaaaag aattagtacc ttttttacca
     3901 ataccagtaa ttgaaagaaa agaagataag tatgtactaa attatgatag agaaaaatct
     3961 attgggaaaa ttaaaaattt ctatggtaat tttggagttc ttgtaagagc ttacacatat
     4021 atactaacta tgggaaggga tggacttaaa gaggctagtg aaatggcagt tttaaatgct
     4081 aattatataa aggaaagtat aaaagatgat tatattttgc caatagatac cttgtgtaag
     4141 catgagtttg tattaggtgg attgccaaga ggagaggcca atataaaaac tatcgatatt
     4201 gcaaaaagac tactagatta tggataccat cctccaacaa tgtactttcc tcttattata
     4261 aatgaggcac ttatgataga acctacagaa agtgagagta tagagacatt agatagtttt
     4321 atagaagcta tgaaaagtgt tgctaaagag gcaaaggaag aaccagaact attgaaaact
     4381 gctccacata atactttggt caaaagagta gatgatgcaa gggcagtaaa gaaacctata
     4441 ctaacatggt cacaaagata aatagttaaa aagtagagat ttaaagtata aagagaatta
     4501 aatttataag aaaaagctat ctaacttaat ctagtttaga tagctttttt gtatattagt
     4561 atatatggta tttcctatat aaagttttaa attgtattag tgcttatata gaatattgat
     4621 atgatgatta tgtatagata ttaataagaa ttgttaaatt tatcttgcct taactttatt
     4681 tactctttta tattaagaaa aacttaacta aaatatagcg aaattcataa aaatttagga
     4741 aatcttaaga attataatat ttaaatcttt agatttatat tttattatat aaactataga
     4801 ataataaaat aaaaaaattg agaattttat aatatatttg tagatttgag tataaaaaag
     4861 tgctaagttg aaaggagtaa aatatggata aggttaattt agaaaggtat agttgtgata
     4921 taaatgttgg tttaaataaa aatcaacttc aaagtagaat agatgataat ttaattaatg
     4981 catttgataa tggaaaaacc aaaacatata aacaaatttt taaagacaat atatttacac
     5041 tttttaatct cataaatatt gttttagtct gtttattagt ttcagttggt tcttttaaaa
     5101 ataccttatt tatatttatt gctgtaatta atactattat aggagtaata caagagataa
     5161 gagctaaaag attattggac aatctatctg ttatagtttc aaataaggta tctgtcataa
     5221 gagaaaaaga gaaaaaagag attgatgtac aagaattagt attagatgat attatgattt
     5281 taaaaacagg taatcaaatt tgtgcagatt caaaagtttt aaatggaaaa ttagaggtaa
     5341 atgagtcttt ggttacaggt gaatctgata ttattataaa aaataaaggc gattttttat
     5401 attctggaag ttttgtagtt tctggagaag cttttgtaag agttgaaaat ataggaaaag
     5461 ataactatgc aaataagata gctaatgatg caaagatttc taaaaaacat aattctcaac
     5521 ttagaaattc attaaatgca atattaaaaa ttgtaggtat aataataatt cctctaggaa
     5581 ttatattgtt tgcaaaacaa cattttggaa atggagattc tatagctaca tctgtagtag
     5641 gtacagtggc agctgtaaat ggtatgatac cagagggatt aacattactt acgagcattg
     5701 ctctagctgt aggaactata aaacttgcta gtcataaaac tttagtgcaa gaactgtact
     5761 gtgtagagac tcttgctaga gttgatacac tatgtcttga taagacaggt acaattacag
     5821 aaggtaaaat gcaagttgat gatatagtaa tgttggaaga agtggatatt aatgaaataa
     5881 tggggaatat atgtaattct ctcaaagatg ataatgcaac actaaatgca ataagagata
     5941 agtataatgt tttagatacg tataaagcta agaatttaat accattttct tcagaaagaa
     6001 aatacagtgg ggttaccttt gaaaataaag gtacatattt attaggtgca tttgaattta
     6061 tatttaagga aaaaaataaa aaacttagga tagaagttga aagatattct aaaaaaggaa
     6121 acagagtaat agttttagct catagtgatt cttatatgga agataataat attcctgaag
     6181 atataaagcc actagctttt atattgatat tggataaaat tagagatgaa gctaaggaaa
     6241 ctttagaatt tttttacaat gaaggtgtag atataaaaat tatatctggt gacaatccta
     6301 taacagtaag tgaggtagct agaaaagcag gtcttaaaac agctagtaat tttattgatg
     6361 caacaacact taaaaatgat agtgatatat acgaagcagt tgataaatac agtgtttttg
     6421 gaagggtaag cccacaacag aaaaaacaaa tgattttagc attaaaaaaa caaaatcata
     6481 cagtagctat gactggagat ggtgtaaatg atgtgctagc acttaaagaa gcggattgta
     6541 gcattgccat ggcatctgga agtgatgtta ctaagaatgt gtctaattta gtcttattag
     6601 attcaaactt tgcatcaatg ccaaaggttg ttatggaggg aagaaaagta attaataaca
     6661 ttcaaagagc atcttctttg tttttagtaa agacaatttt ttcattcttc ttgtcgttat
     6721 taacattatt cttcttcagt agacagtatc catttgttcc tatacaatta tcactcattg
     6781 gggctgtaac agtgggtata ccatcattct tcttagctct tgaagcaaac aaaagtagaa
     6841 tttcaggaaa ttttttactc aatatattaa aaaatgcttt accaggagca ctatgtgtga
     6901 cagtaaatgt aattattgtt tacaatatag ttgaatattt aggttatagt tcaagtgtat
     6961 tttccacaat gtgtgctata ttaactggtg tatgtggtct tttaatatta agacaacaat
     7021 gtttgccatt taataaagaa agattatttt taatagtaag tatgactatt gcatttgtta
     7081 ttgggattct atttttaggt ggattattct catttgtagc attgagtaga gaattaattc
     7141 tcataactat ggcattagca atattaatgc caattatgat aaaagttatg ttatttgtct
     7201 tagatgagat attagagaca attgttccag atttaaatta attttaaaat gatataaata
     7261 agagcagttt tagttgaact gctcttttta gatgtgttta attttatgta agaatatgta
     7321 tttttattaa tcagctatat atgagcaaca gtagtcaaca acttcattaa cctttaagtc
     7381 aaaacttgaa tcactaggaa cattaaattt ttgaccttct ttataagtta caaactcatt
     7441 gcttccaggc agttttacat ccatacttcc agcaagcatc tccattattt cttcttctct
     7501 agtagaaaaa gtgtattctc ctggcaacat taatcctaaa gtttttcttt ctccattagg
     7561 cagtattatt actctgctac ttaccttacc atcaaaataa acgtttgcct tctttactgc
     7621 agtaacattc ttaaattcac tcattagata aaacctccaa aattatttgt acatatatat
     7681 tatataaaaa taatgtaaaa aatgctatat atttacaaaa aaatttagta tttattaaaa
     7741 aaattaataa gaataaaaaa gagatgaaat tgcttaaatt gataaaaaat ataaaataaa
     7801 ttgcaaagta agcaagaaaa gaataagttg aagagttata tattataaat attaaaaatt
     7861 ttagtattaa taaaaaagat attacattaa tatgaaaata taggagatta caagtgtttg
     7921 ataattacag tacatgttag atatatcatt gtaaattaac atagctttga atataataat
     7981 gttcagaagg caatattttt gaggtctgaa tccaataata atatagtctt atgattgtag
     8041 atatcaaaaa atgtaagtta ctaggaatac gttttgtaga tttatatatg attaataact
     8101 aaagctataa ataaacaaaa taaaaagagt atgtttcaac ctatagcttt tagatttaaa
     8161 atatactctt ttattaatta tagttgtata aattaaccat aatatataat taaaattttt
     8221 attataatta tatattaggt tcttcaatta gttttccaca tacatatcta tgaatgatat
     8281 ttctatcatc accaacatag ataaatcttt caagtctttc aagtatagaa taattgtcat
     8341 gatttaaatt tgaatcatca ataatcaatg catcaaaatc ataaccttct tcaaagctac
     8401 caacttttcc aaagaaactt ccaccacttt tggttgcaat ataaaaagcc tcagataaag
     8461 ataagaaatt atacttctta ccagaattaa cccaatataa tttagataat tgtattgcag
     8521 ataccattgc tttaaatatt gacaatgtat gtccaccgct tatatctgat ccaagtgcta
     8581 atctaatatt attatcgatt aattttctga taggcatagc accactacca acattaaagt
     8641 tagaagctgg acagtgaact gccataacat tattttctct cattaaatct atttcatcct
     8701 gagaacaatg aacacaatga gccattaaag taggagtttg accaaataaa ttatatctat
     8761 tatatgcatc tccatagaat ttagattcag gttctagact tctaacccat tcaatctcat
     8821 cataattttc agataagtgt gattgaatag gaatattata ttttaaacat aaatctccta
     8881 aaccttttaa aagttcactt gtacagcttg gtataaatct aggtgttatt attggtttta
     8941 caatagaatc tgtatcacta tacttattta taatttcttc tgtatcagca atagaatcca
     9001 gagtgttttc taataaagta tcagggcaat tcatattcat atttacttta ccaacgtatg
     9061 ctccaagacc aacttctttg aatatatcca taagtttcat agaggattct ttatgtactg
     9121 ttgcaaacac tgctattctt gtagttccag atttccaaag ttcttttaca aattttttat
     9181 acatttttgt tgcatattct atatctttat aatttgattc ttctggaaat gtataagttt
     9241 caagccatgg caaaagctct ttatccatag ccattccaag atttttaaat tgagaggcat
     9301 gggcatggag gtcattcata ccaggtatta taagcttatc agtgtaatct actactttta
     9361 ggtttttgta ttccttaggt atctctttaa atattccctt tactttacct ttttccagta
     9421 taatataact attttcaaat actgtgaaag tttctgaagt ttttgtaaaa attatatttc
     9481 cttttaatac ttttaacata taggtctcct tagttttaaa tttgttttaa tatatgtagg
     9541 taaaattttg ttttacaaat caaaatttta ccacatgtat tatatcaaat ctaaactata
     9601 aatactatta gataatagca aatattagtg aaatacagtt gtttactatt attatatata
     9661 actgacattt aagtgacatt taagaaaaat ataatgccta cttacataaa atggaatgtt
     9721 atttaaagag aactttgatt atattttcag aagctttttt atccatatcg tttaaaatat
     9781 gagaatatct atttatagtt atttttatat tagtatgtcc taatctttca gagatgattt
     9841 ttatattagt tctagctaga agaagaacta ttagaataga taatatagta agtatttaca
     9901 aatatgtagg tgttcttaaa ttgataaatt attccatttt aattttatag tttgaatttt
     9961 atgatataat aaaaatatat aaattttgca gtgagcgata tttttgataa agtagggttt
    10021 aacagttgca atgtaaggga ttgagggtgt atgataaatg ttatcaattg cactactcat
    10081 ggttcactgc aaatttgaga gagttgtatg tgtataagta ctgaaaatac ttagtttatt
    10141 ttggggtttt atattaacta agtggtatgt aaattcccca tgtccaagtc cagtaacacg
    10201 ctttaaagag ggttttatat taactaagtg gtatgtaaat atatgcatct gttagctttt
    10261 tatttctcct aatgccatgt tttatattaa ctaagtggta tgtaaatcta atcttaaagt
    10321 atctttggca agctccaagt caaggtttta tattaactaa gtggtatgta aatgaaactt
    10381 ataaacctgc tccagattca ggaacagtga gttttatatt aactaagtgg tatgtaaatc
    10441 gacaagcaaa aacaaaaaat ttacgaaaat aaccgctagt tttatattaa ctaagtggta
    10501 tgtaaataca tttgaagaac aaaacttaga cttttataac gcacgtttta tattaactaa
    10561 gtggtatgta aatctagtac ccttccacca gcatcaattg cattatcaac cgttttatat
    10621 taactaagtg gtatgtaaat atataagcag gaaataaacc agcctttttt gctaatggtt
    10681 ttatattaac taagtggtat gtaaatcttg ctggatttga atttaatata gaattttctc
    10741 aggttttata ttaactaagt ggtatgtaaa tagtcattgt ttgttatgtt tatcttatta
    10801 ccctgtaagt tttatattaa ctaagtggta tgtaaatata aacataaaaa acattgatag
    10861 taatatatac tatgagtttt atattaacta agtggtatgt aaatagctac ttaaacacat
    10921 cagtaatggc aaattgtggg gttttatatt aactaagtgg tatgtaaatc aaattgctga
    10981 caacactgaa agcattatga acgctagttt tatattaact aagtggtatg taaattgagg
    11041 tattaagacg ttgaacattt gcagagcata agttttatat taactaagtg gtatgtaaat
    11101 cgaaaaatac attcaagtcc aaagccattg gcgaaacgtt ttatattaac taagtggtat
    11161 gtaaattctg gaatatgaga agtttctgta gtaccataaa gtgttttata ttaactatgt
    11221 ggtatgtaaa ttagaaaata acgtcagtag tattagcagg taaatctaaa gttttatatt
    11281 aactatgtgg tatgtaaata acactttaaa aactaatgta ttcagtataa gagattgttt
    11341 tatattaact atgaggtatg taaatcttat acttagttag aactatatat cgacacaaat
    11401 atgctttata ttaactatat ggtatataaa ttgcaatttt tattcgttgt ccaatatctt
    11461 tgaaatttgt tttatattaa ctacgtggta tgtaaatttg caacaagtat aggtaaaata
    11521 ccccaataat ttatacagca ttttctcctt taaaatataa ttatttttta gcatttgtag
    11581 taaataatta ccagataaca ttgactttag ttttaatgat taaaatataa aagtagaata
    11641 attataaaaa gtattgaaaa atttatacat atatataata aaacttaatg acaagatatt
    11701 agatataaaa aataa
//
LOCUS       DAEMIK010000135        11681 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEMIK010000135 DAEMIK010000000
VERSION     DAEMIK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.137"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..678
                     /locus_tag="KPK87_003037"
     CDS             <1..678
                     /locus_tag="KPK87_003037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539161.1"
                     /translation="RSQYSFRHESNINNTRINESIVKKNETAGKDTYLSNIMKQKQEL
                     NDRIRDLKDRQEIYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNRIIEDKNKSTML
                     DENSTYLNTNDEKESLIAKEFNEKVEEEILNDEKLEELEQKKDYKEDSNKKETVSEDL
                     SLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIFDKTDEQK
                     RRINTLV"
     gene            856..1746
                     /locus_tag="KPK87_003038"
     CDS             856..1746
                     /locus_tag="KPK87_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539162.1"
                     /translation="MKKNILTTVITMIIIFMMSMTVMAEQYQADETSSLKKQSENESN
                     QTSVGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALAGGSYQRIGFGKLDPIEVIADGDWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADAKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKYA
                     TREMTTYYDVKALSILGFNQ"
     gene            complement(2143..3099)
                     /locus_tag="KPK87_003039"
     CDS             complement(2143..3099)
                     /locus_tag="KPK87_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2539163.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISILDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRITDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSKILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3801..4796
                     /locus_tag="KPK87_003040"
     CDS             3801..4796
                     /locus_tag="KPK87_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2539164.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLLNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSHKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4774..5637
                     /locus_tag="KPK87_003041"
     CDS             4774..5637
                     /locus_tag="KPK87_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG2539165.1"
                     /translation="MSIMSNYNEASSNSSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKIFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5634..6428
                     /locus_tag="KPK87_003042"
     CDS             5634..6428
                     /locus_tag="KPK87_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG2539166.1"
                     /translation="MIKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6713..8092
                     /locus_tag="KPK87_003043"
     CDS             6713..8092
                     /locus_tag="KPK87_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2539167.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILIFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIC
                     KYKNRYKYM"
     gene            8542..9255
                     /locus_tag="KPK87_003044"
     CDS             8542..9255
                     /locus_tag="KPK87_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539168.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLTNDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSEVIKVGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMDQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9532..11070)
                     /locus_tag="KPK87_003045"
     CDS             complement(9532..11070)
                     /locus_tag="KPK87_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2539169.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGAGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINKDTTISLYLHTAFEKLYKIEKEMN
                     LLYQKSAISGNEQYLIKASDYQEQLIANNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSDYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4244 a         1338 c         1789 g         4310 t
ORIGIN      
        1 agaagtcaat attcatttag acatgaaagt aatataaata atacaagaat aaatgaaagt
       61 atagttaaga aaaatgaaac tgcaggaaaa gacacttatt tatctaatat tatgaaacaa
      121 aagcaagaac ttaatgatag aattagagat ttaaaagata gacaagagat ttatactaaa
      181 aaaataaatg aagcaattaa gaacttatat aaatcagaaa taagagaaac aactaataac
      241 ttttctaata tagaaatagg tattaaaaat agaattatag aagataaaaa taaaagtaca
      301 atgttagatg aaaattcaac ttatctaaat acaaatgatg aaaaagaatc tttaatcgct
      361 aaagaattta atgaaaaagt tgaagaagaa atattaaatg atgaaaaatt agaagagtta
      421 gaacaaaaaa aggattataa agaggattct aataaaaaag agacagtatc tgaggactta
      481 tccttagtag gtaaaactcg tgaagagctt gaaaatatgc ttaaaaattt tataaattta
      541 acacaagaag aaataatgaa acttgagtcg agaatagaaa agttagataa agatgctgaa
      601 gaatacaaac aaaattcaaa gactaatata tttgataaaa cagatgaaca aaaaagacgt
      661 ataaatacat tagtttaaat agtctgtttt aagaaaataa atttatgatt aatgaaaatg
      721 taaagataca atttcaatac atttctgcta aatcaacaat ttgtgaatct gacttaattt
      781 taattatact aatagccata tatttataag ttgataattt tatgttttaa aaagcattaa
      841 ataaggggga gtaatatgaa gaagaatata ttaactactg tgattaccat gattattata
      901 tttatgatga gtatgacagt aatggcagag caatatcaag cagatgaaac atctagcttg
      961 aaaaaacaga gtgaaaatga aagtaatcaa actagtgtag gtacaatgga tggcagagtg
     1021 caaacaaatg caataagtaa aggtgctaat tatgacccga agtatcctct taaaggaatg
     1081 ttaacacaac ttggtcttaa tacaacaggt aaagaggggt gggatttact ctatggagga
     1141 aatccaaatg gagttgagct tcgagcagat ggtaaatatt attatataaa taagggtatg
     1201 gaagggctag gattttatgc ggcagcatta gcgggtggct catatcagag aataggcttt
     1261 ggaaaacttg accctataga agtaatagcc gatggagatt ggatttctta caaacaagct
     1321 caagatacac ttacagtaat caggaatttt ttaaatagtt ttgactggag aaatgcatct
     1381 gagatggaaa gagctaatag agctgcaaaa ttggtaaccg aagcaaaata tgcagacgcc
     1441 aaatattgta acatagtcta tggtaatctg gttgataaac gtggtgtatg tggaagtttt
     1501 gcaagtagct ttcatttatt aacaaggtta atgggaatgg attcactatc tatattaaat
     1561 ccatcattaa atcatgcttg gaactatata caaattgatg ggaaatggta taggtttgat
     1621 gggtctgaaa tatcagcttt tggtggatca ttagactttg attaccgtaa attaaagtat
     1681 gcaacgagag agatgactac ttactatgat gttaaggcat tgtctatatt aggatttaat
     1741 caataaaaat agggaaggct tcttatggag ccttttttta ttgatttgaa atttaataag
     1801 gaattttata ttaaaattta atcaataaaa gcaaaaaagg ctctttataa agcctcttta
     1861 agtgtttttt tcaagtaaaa ttttttatat caacatcagt tttaatcaaa aaatataaca
     1921 ttttggactt acttttaaaa ataaaattat taaaatataa tattaatcat gttaataatg
     1981 tttttagttt attatataat agtggttatg ttgactattg tagttttatt aaaaatagtt
     2041 ggtttatata aatatatgca aagatagctt gtggtttact ttatttatta gtatatagta
     2101 aaagatactc tgataaattt gcttatttta atatcttttg cactaaaacc aactaaagtt
     2161 tcctttatta tttcgttttg aataatacaa agcataatcc gattgtttgt ataaagactg
     2221 gaaagtatca gagttatttt ctaatttaga tatacctata gaacaagaat tactctctag
     2281 gcataattct aaaactttct ttgctttatt ttcaacattc tcttttgatt gattactata
     2341 ttcaaaaaca gcaaattcat ctccaccaag tcttccaata atagcatcat ttacgaagac
     2401 tttagtcaaa atctttgaaa aatgtattag tatttcatct cctcgaacat gaccaaagat
     2461 atcatttacc tgtttaaaat tatctaaatc aaagatgtaa aagtatccat gtgaaacagt
     2521 tttaagcatt tcttttatgt gtacttcaaa aaaatgtcta ttgtatagtc cagtcataag
     2581 gtctttttct gattttagtt taaattcatt tgccattgct agtgcttctt gtgctttttc
     2641 agttattgtt ttagggtaat actcatcttt catttggtca aagtttggaa tagaatggtg
     2701 aacatggata agtttaatca tatcatctgt aattctgtat accatagtaa atcgagtatc
     2761 catttctaca tagatatctt tttctaggta atctttttct attacccaaa gagtaccata
     2821 tactataaaa gcttcgtctg taactttggt acatgaatac cactcatcta taatctcaaa
     2881 gtctatttgc tctgattctg tttggtcttt ttcaagacct ataataaatt cttctaatgt
     2941 ttcgtaaaat tcatgttttc ctgttccaat taatgcaagg ttgtcatcta atatggaaat
     3001 aataaattta aggttttctt cttctggatt taaaatataa atatgccaaa ttttttttga
     3061 taaatccctt aattgttttt gtattgtatt attttccata gattaccccc ttaagattta
     3121 cagtttatat tttaatgtat tataagttga actcttatta caatattatt ataatataga
     3181 actaaagaaa ttacatcctg tataggtaaa agtatatctt ttacaataaa aatattacag
     3241 taaaataacc cgttttaata tacgtattat atttttaatt tataaatgaa tattataaat
     3301 gaaatttaaa ttcaaccctg aagaaataag ctaaatgtat tagtatataa atccatactt
     3361 gaacataaat aacctttatg atatgatatc aataataata ttatgagagg agtgccttga
     3421 tgtcggagtt atataatggt aaccaacaat taaatagttg gagacacata tattctgtat
     3481 aatcagaaat tttaagtgta tttttaatac atttaatttt tgtgtcttat tctgccttta
     3541 tagataactg acatatgaca acaaatagta tttaaaatat atattacttt gttaaattat
     3601 ggtgtagtag gctttaatct gctacacttt tattaatttt gtatagtatt attttgataa
     3661 ctattttgaa atagagagca ttaatttttc tagtccacag gagttttctg tttttgtgct
     3721 gttaaatata agcacagaaa aaggcagata tatacctgtg gacttttttt tataaattat
     3781 gaaatatgga ggtagttaaa atgagcataa ttgaacttaa tgaactgact aaatcttata
     3841 gaagatttga gaaaaaggca ggatttatgg ggagtgtaaa gtctctactt aataggaaat
     3901 atacagaaaa aacagctata tcgtcatttg atttaaagat agaagaagga gaatttgttg
     3961 gattaatagg tcaaaatgga gctggcaaaa ctactcttat taagatgtta actggtatta
     4021 ttgcaccgtc ttctggagaa atatctgtta tgggttatta ccctaacaaa ttagaaaata
     4081 attttaaaaa acaatatgct gttgttatgg gccaaaaaag tcaactattc tttgaattga
     4141 caccagcaga tacgtttctt ttatttaaag aattatatga tatatctaat gaagaatata
     4201 aaaaaaactt ggattacttt atcgaattat ttgaggtatc tgactattta aatgttcaag
     4261 ttaggacgct ttctttagga gaaagaatga aaatggaact aatagtagcc cttctacata
     4321 atccaaaaat attatttttg gatgaaccaa ctataggctt agatgccata gcccaaaaac
     4381 aaattaggta tttcctaaaa gaagtaaatg aaacaaaagg aactactatt atacttactt
     4441 ctcattacat ggaagatatt aagagtctct gtagtagaag tattgttgta gaaaatagcc
     4501 ataaaatata tgatggttct acagagaaac tatttaatca ttttcaaaaa tataaaaaaa
     4561 taacagttag tttttccaat gaaactaaca ttgtatttcc aaaagattgt aaagttgttg
     4621 aacaaacaaa tcataagaca tcagtaattg tgccaaaaga aagaggcaat gaaacgatta
     4681 aatggatttt tgataaatat gatgttaaag atgtaatgat tgaagaagaa gatataggta
     4741 gtattgtgga gagaatatat aagaaaggaa gtgatgagta ttatgagcaa ctataatgaa
     4801 gcaagttcaa attcttcaat gttttataaa tttaaacaga gtattagaag gtattatgaa
     4861 gtagccaaaa ttaactttaa agtgcaactt atctggagat ttgatgtggt ggttaatatg
     4921 gtgtttacga ttgtaaaaat tttatttgct tatattttat ggaaagcaat ttttggtgaa
     4981 aataaaatag tctctggttt tacatttaac tctatgttgt cttactatat aataagctca
     5041 tttttagcag gtattgatat gtcaaagagt accagtgaag agatatgtaa tcgtgtaaaa
     5101 ttgggcacat tttcaaagta catgattatt ccaattaatg ttcaaaatta ttttattgct
     5161 aaaaattttg gaatgacgtg tttttatttt gtatttaata tgatttctgc atttgtatgg
     5221 atttttatat ttaaaataga ttttgccttt acatcaaatc cagttcaaat attaatttca
     5281 attttgatga tttttctagg gtttattttt atgatgaaac tccacttctt tattggaatt
     5341 ttgtcattta aatttcttga gattagtttt tttagaatga taattgaaaa tattgttgaa
     5401 tttattacag gaacaatgat accacttgta ctcttaccag aaactatagt aacagttatg
     5461 aagatatttc cattttatta tacaacatat ttgccatcta tgttactaat aggtagaaac
     5521 agcaatgagg caatacaagg attaattatt ctagcattat gggttttatt tttttctgta
     5581 gtaaataaag ttatgtataa aagaatgaga acattatttg atggggtggg tatatgatta
     5641 aaaaaaattt gaaaataata tttatgttat taaaaatgaa attatcaaag atgatggtat
     5701 ttagatttga tttttttgga gcattttttg tagatagttc attatttata ttacaacttc
     5761 taatgtttaa tagtatatat tctcatgtag actcaatagg gggatggcaa caaggagaga
     5821 tgttaatatt tataggaact ttctcattaa taaatgcgat aaatatgacg attttctttt
     5881 ttggtattta tgatattcca cgaaaaatcc aggaaggaga attagactac tatattacaa
     5941 aaccattaga cccactattt aggttaacat ttgaaaatat taatattggt tcatcaccat
     6001 taattctagc aagtatactt attgttttat atggaatatc tgagttaaac tcagagataa
     6061 ctttagttat gataataata tatattttca tggtgttatt gatgacatta cttttttatg
     6121 atgtatgtat catacttaga acagtttcat tttttgttat atcatcttct gcgataatga
     6181 gattagaaga taatttattg ccaatgaaca tgaaaattcc tggtgtaata tatgaaggtg
     6241 tttttaaagt attattctat ctaatcttac catatggaat tatgtcgaca attccgactc
     6301 aaattttggc tgggacaata acaattaatg gattaatata ctcaatttta attgtatttt
     6361 tgtttacatt gtttatgtgg agattttgga agttaggtat gagacattat aaaagtgcaa
     6421 gtagttaata aattggtaaa aatagaaaat gtataattat atataaatac gtttcatata
     6481 acactaaact atgtaaacaa gtatttgcac taaaggcact gaaagctata aaattattaa
     6541 agtaaggatt taaaagttac tttagtagaa atataattgt gtaaaaaagc aagattaatt
     6601 aaaactatat ccgaataatt agatataatt tttatgcaga aaggaggtct gtaaatgtct
     6661 aattgtttgg aatatatttt aaaaataatt aatgtacaga aaaataaaca aaatgagtga
     6721 tgtatattat tcagaaaatc atgaacaaga taatctttat gagcaagata atttactggc
     6781 acaaagattt aatttattta ctctattgaa gtttgcattt cctactattt ttatgatgat
     6841 ttttatgggg ttgtatacaa tcgtcgatac tatatttgta tcaagattta ttaatacaaa
     6901 tgctctatca gcaattaata ttgtatgtcc aattattaat cttatagttg gaattggaac
     6961 tatgcttgcc actggaggaa gtgctattgt agcacgtaag atgggaaatg gtgatattga
     7021 gggagcaaat aaagctttct ctttaatcgt attatcagga gttgtaattg gggtaataat
     7081 tactggtata ggtattgtat ttatggatta tatcattata tggttaggag caagtgaagt
     7141 tttaattaaa tattgtcgtt catatctttt tattatcttg atttttgctt ctgcgaatat
     7201 actacaagta ttatttcaat gtttatttgt aacagctgga aagcctcaat ttggattatg
     7261 gctagtgtta ggtgctgggt gtgctaatat aattcttgac tatgtgttca ttgttctgtt
     7321 acaaatgggt attagtggag cagcacttgc aactggagta ggatacctaa ttcctaccat
     7381 ttcaggtatg gttttctttt caaaaaataa agggttactt aaattttgta tacccagact
     7441 agataaaaga atattatttg aagcttgttt taatggttca tctgaaatgg tgggtcaact
     7501 atcaacagca gttactactt ttttatttaa tgctatgatg atgaaattaa ttggtgaaga
     7561 tggagttgca gctattacaa taattattta ttctcaattt cttttaactt cgctttatat
     7621 aggtttctca atgggtgttg caccaataat aagttataac tatggaagtg aaaataatac
     7681 gcagttaaat catgttttta agatttgtat attatttatt tcagtagcat ctttattggt
     7741 atttatcttt tctatatcat gttcaccaat cattgtagag attttttctc aaagaggaag
     7801 ccgagtttat gaaattgcaa ggtctggatt ttacatattt tcttttagtt ttattttttg
     7861 tgggtataat atttttactt cgtctatgtt tacagcatta tcaaatggaa aggtgtctgc
     7921 aatattatct tttctacgta catttggatt tataactata ggtttattga tattgccaaa
     7981 tatattaaat ataaatggga tatggttagc agtaccaagt gctgagattt taactttatt
     8041 tttaaccgtt ttttgtatat gtaagtataa gaatagatat aaatatatgt agatattaaa
     8101 taaatagttt aggtagatat tttatgtttt aataagtaat tcaattagtg gtagatagat
     8161 actattgaaa attaaagtta taattgatat aaagaattaa taaaaataac atataaaagt
     8221 ttaaaagggg agttgaactt aaagtttgat tagaaaaagt caattttaag acaactcctt
     8281 ttattttact attgtattaa tttgtatctg taagtgtatt agtctaatat tataaacttt
     8341 ggatgaaatt aattttctat aggttttaat gtatcataca ctttatttta aagaaactta
     8401 tcttaatttt catagtgatt gaatttgtct gttagagatg ttactattag taatgtaaaa
     8461 gcaagagtag tgaatataaa aaaacaagat agtgatactg caagattaga atataataaa
     8521 tttttatgga gagatttcaa tatggataca tattttaata aaaaaatatt acttgttgat
     8581 gatgaaaaag atatagtaga tttaattgaa gaagtactta caaatgatgg ttttaaaaac
     8641 attattaaag cttataatgg tttagatgct atttcgctgt gtaagatagc atgtccagac
     8701 gttgtcattc ttgatataat gttaccagat atagatggta tagaagtgtg taaaaaaatt
     8761 cgagagtttt catactgttc tattcttttt ctctcatcaa aaaatgatga tattgacaaa
     8821 atactaggtc ttagtagtgg gggagatgac tatataacaa agccattcag tccaagagaa
     8881 attgtttttc gagttaaagc gcaattacgt agacaacagt atcaaagtat tgtaccatca
     8941 gatagtgaag ttattaaggt aggagatatt actattgata tagaaggaaa ccgtgtatat
     9001 aaagatagaa atgagattga gttaactgga agggagtatc atcttttaag ctacatggat
     9061 caaaatgtaa ataaaattat aggaaaagaa agactctatg aacaagtatg gggagtatat
     9121 agtagtattt gtgacaatac aattatggtg catatacgac atataagaga aaaaattgaa
     9181 gataacccct caaatcctaa aatacttata acggttaaag gactaggata taaacttgtg
     9241 aatagaatcg attagaatag attttaatat caattaagat gtctcaaatt gaatttttta
     9301 gttaatgagg cattctttta tgtgtgaaat caaatgtatt ttcgttatct caataatgaa
     9361 gaagaggtct atctctattt tgagatagcc tcattaagtt tatatcctgg caatgtactt
     9421 tatgttctaa taaatattta ttttttagtt tatttgcagt gaaacattaa ctagaacatt
     9481 aaaatttgca ttgaaatacc aattaaataa tattgtatat tgtgtataaa cttatactaa
     9541 ttgtttttct atattaaata ctttatcaat ccaaccttta taaaattttt cttcatggga
     9601 aactaggatt atacttcctc taaactgttt caaagcattt tgtaaagcat cctttgtctc
     9661 tgcatcaaag tgattggttg gttcatccag aataagaaaa ttacaaggag ataacatcat
     9721 acaacataat ttaaccttag attgttcacc accacttaaa gtagatacac ttcttgaaac
     9781 atgctcttcc ttcacaccac atctggctag atgacgtcta atctcttttg tatttaattt
     9841 tggatattta tctgctacta tttgaagtgg ggttttatca ggattttccc attttaaatc
     9901 ttgctcataa taaccaattt taacttgttc tgaaaactgg aaatctccag atatacgagg
     9961 aatatctttg actaatgtct ttaataaggt agatttaccg attccattaa aaccagttat
    10021 aactattttt tgacctccat caacagaaaa atttaatttt ggaagtaaag aatagtagta
    10081 tccaacttca agatttgtta tagtaagagc attttgagcc gatatctcaa tttctgaaaa
    10141 ttgaatattt ggttttcctg taaaactagg aggagctatt ctttctattt tatccaattg
    10201 tttttgtctt cctcttgcaa tttttgaatt tactccagct ttatttttac gtataaatgt
    10261 ctcttctttt tctattttct tttgttgtgc ttgatacctt ctaatataat cttctcttaa
    10321 atgtgatttt tgtttaagga actctgaata ttttccatga tatttcttaa tcatgccaaa
    10381 ttctatatca agaatacctg tagaaatttt ttctaaaaag tcaaaatcgt gggaaactac
    10441 aataaatgcc ccattaaatg tatttaaata atctgagagc cattcaacat gttctttatc
    10501 taaaaagttt gttggttcgt ctaataatag aacatcatgg ttagaaagta gtaattttgc
    10561 taaaattacc tttgcccttt gcccaccact aagctctcca acaacacgat tcattcctat
    10621 tgaatctaat ccaagaccat ttgctatttt atttatttca ttatctatag aatagaaatt
    10681 atttgctatt agttgttcct gataatctga tgctttgatt agatattgct catttccaga
    10741 tatagcactt ttttgataga gtaaattcat ttccttttct attttataaa gtttttcaaa
    10801 tgcagtgtgt aaatagagtg atattgttgt atctttattg atttctgcat attggtctaa
    10861 atgtcctata ttgatattag gttgccactt gatttctcct gaatctggaa caacttctcc
    10921 caataaaatt ttaattaatg tacttttgcc tgcaccattt tgaccaacta cacctatatg
    10981 ttcacctttg aataaatcaa agttagcttt ctcatacagt gatttatcca taaaacattg
    11041 tgataaatta gttactttta ataaactcat aattcaaact ccttttcttt tattaaaaaa
    11101 tttatggaaa taaaaaaagc agaggaatgg cattgtaaaa tgccgacctc tgcttttgga
    11161 aaaacaaaag gtcatagaca agcattgttg tctatgacca gaaaatctat attatctatc
    11221 ggcaaatagg taattttaaa taagcatagc caaaaaacta tactaaacct atatttgccg
    11281 atgaagatag aaagctttaa gctttcagat taacctctac acaacgcttc atcgtataca
    11341 tttgtgtaga gttaagtttt ctaacaagtt taactaattc tatataaatc ataattattc
    11401 acatcctaat cttagtatta attctagttt atctatattt aaattgtact tttgtacttt
    11461 gtggattatc ttatttattt aaaatatgtt ttattaatta tagcttaagt tatgttatca
    11521 taatacttag ttatagtcaa taagaaagtt atatttacta tatattatat taatttagca
    11581 aatctatcat tacaaagtta tttgaagatt cgctaccaat aaagttttat agtctgtaag
    11641 aaaactaaga ttatgagttt tataattttg atatagggat a
//
LOCUS       DAEMIK010000136        11520 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEMIK010000136 DAEMIK010000000
VERSION     DAEMIK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.138"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            355..513
                     /locus_tag="KPK87_003046"
     CDS             355..513
                     /locus_tag="KPK87_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539170.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(800..1504)
                     /locus_tag="KPK87_003047"
     CDS             complement(800..1504)
                     /locus_tag="KPK87_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539171.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1495..3102)
                     /locus_tag="KPK87_003048"
     CDS             complement(1495..3102)
                     /locus_tag="KPK87_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG2539172.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3356..4534)
                     /locus_tag="KPK87_003049"
     CDS             complement(3356..4534)
                     /locus_tag="KPK87_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539173.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4760..5254)
                     /locus_tag="KPK87_003050"
     CDS             complement(4760..5254)
                     /locus_tag="KPK87_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2539174.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5255..6016)
                     /locus_tag="KPK87_003051"
     CDS             complement(5255..6016)
                     /locus_tag="KPK87_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2539175.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6718..9552
                     /locus_tag="KPK87_003052"
     CDS             6718..9552
                     /locus_tag="KPK87_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2539176.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNTDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9595..10839
                     /locus_tag="KPK87_003053"
     CDS             9595..10839
                     /locus_tag="KPK87_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2539177.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLDIKNSVCFKA"
     gene            complement(11334..>11520)
                     /locus_tag="KPK87_003054"
     CDS             complement(11334..>11520)
                     /locus_tag="KPK87_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539178.1"
                     /translation="QAVQTDLDNVSVLVKAGAVSETTATDSAANVTFEVKKHVSDPIS
                     TIGKISSGLSSLFNILK"
BASE COUNT         4140 a         1850 c         1430 g         4100 t
ORIGIN      
        1 tatttagatt gttactttga tatattatat aatatcacaa aggctgatat aaaaagacaa
       61 aaagggcaat ttttttatat caaaaaaaat gttaaattga tattatccat atagatgcta
      121 tatttaacaa ttctacttac ttcccttaca cttattagca tatagttatt aagaatttgt
      181 tgcaaaacaa cacttagcac actttaaaaa gtgtggtcta ataagtaatc aacgaagaag
      241 acataataaa gcataaaata tgatatactt ttccacgcca tcaaaactga ggattttatt
      301 attaatatat gctaaaatat gcttaataaa ttaaatttta atgggagaat agttatgaaa
      361 atatttagtt tcttgttgtt tgtgatagga atatgtttat gtattgctgg atttatagga
      421 cattacccag tatttgttgg tacaacattg agttttcttg caattgcaat gatagctgta
      481 agacaagtca aaactaagaa aaaaatgaaa taggcaaatc ctaactttac aagttaatat
      541 tacacaagta attagtctaa aattggatac tttttctatt acagaaatac atcatccact
      601 ttgtattttc ttaaaaaaat aaaatagcta tgtaaattca agattagata gattaactct
      661 ctatccattt cattgtaaaa tatacatagc tatttgtatt cgatttaaaa tacttatatt
      721 ttcttacata acaaataatt tctatctgat tattactcat attcatttag taaccactca
      781 gcacctaatc atttagtatc tattcatccg ccatacggta acctacccca acctcagtca
      841 aaatatattc tggctgagca ggattctttt caattttcct tcttatactt gccataaaaa
      901 ctctaagcga ctgattttca ctttcatagt aatttcccca aactttatct attataaact
      961 tatgggttaa gactcttcct gaatacttag acatcaactc aataatctta tattcgattg
     1021 gagttaaatg tatttcttca ccattaattg acacaatatg attttcataa tctatcttta
     1081 ggtttttaac ttcaaaagac tttggagcat cattatttat attatcctgt tgagctttat
     1141 ttctaagagc cacacgtact ctagccaaaa gctctgttac actaaaaggt ttagttaaat
     1201 aatcgtctgc tcctccatct aaagcttcca ccttatccct ctcatgctct ctcgctgaaa
     1261 caacaattat tttattagtc tttgcacatg ccctaactcg tcctattacc tcaatcccat
     1321 caatatctgg tagtcctaaa tccaaaatta tcaaatctgg gtcaagagaa tgtataagtg
     1381 atatagctgt tcctccatta tctgtctcta cacacctata attttgagtt tcaagagata
     1441 cttttataaa tttacaaatt ggcttatcat cttctacaat tagaactaat ggctttattt
     1501 ccacttgtct ttcccctcct catcatacat aggtatgcta aatctaaatg tagctcctcc
     1561 agaaggatta ttaaaggcga caatttctcc accatgagct tctactatag atttacatat
     1621 ctccaatcca agaccaatac ctcttctttc aagatttcta gacttattct tagtataaaa
     1681 cctattaaaa atattcccga tatcttcatt tggtattcct tttccattat ctgcaacttc
     1741 aaatagtact tgatttgact ctttttttac agttagttct atcttagaat ttttaggtgt
     1801 atgccttata gcattatcaa ttagatttac taacacttgt tctattaaca atacatccac
     1861 atgaacgatt attatctcat ctggaatatt tgtttttatg tctctactat tggcaaactt
     1921 tttgactctt aatatagatt cagctattat ttcatcaaca acctctggtc gtttttcaat
     1981 atccaatttt ccttcatcta ttctggtcat actaagtata ttctcaacag aatgagttag
     2041 ccaactcgta tcttcatata tatttttcaa taattctact ctagtttctt tatctatcac
     2101 atcatcattt tcaagtattg ttgaagatga acccaatata cttgtaagtg gagttcttaa
     2161 atcatgagaa attgacctaa gcaagtttcc tcttaatctc tctctctctg cctcaagatt
     2221 tgctttttta tttttctcaa ataaattttc tctatcaatt gccaacgcaa cctgtgttga
     2281 aactgcttct aatagtatct tttcattttc tgtaataata tctatcttaa aacacgcaac
     2341 acctataaca ccaagaacat ttgattcttg accttttata gggaaataat atgcactgct
     2401 gtttttacaa tattttgtcc caattccaac tggattacct gtcctaaaag cttcttttat
     2461 agcttccttt tctaatactg aatggaatat attctcactt ccatcattgt tacaaatata
     2521 aatacttggc ttacctagat tattattctc atctgcaata gtcactatta cagttctatt
     2581 taatatattt actaaattag acccacaaaa ttcaataatt tgatttttat tttttgcttt
     2641 aagcaacgtt ttgttattct catagagcaa tcttatcatt ttttctctct tatgagaaag
     2701 tctcacttct ctttttatcc tagaagttaa agtacttgtt ataattgctg ccattagcat
     2761 aatacaaaaa gtcataggat aatcttttct ataagctaaa aaagtatata atggatgcgt
     2821 aaagaaaaaa ttaaatgata gcactcctat tactgaagaa agtattccaa aaaaatatcc
     2881 ataggttata cttgtagcaa atagtacacc taatatgtat actagtataa tattagattc
     2941 attaaaacct accttttcaa aaactactga tattccagta gcaattgtca taaccaatat
     3001 agtttttatg aagtccttaa taattatctt tttactgaga tagccatctt catcataatc
     3061 atactctttt aagtcttcat gttgtagtcg ttcctttttc atttactaca attcctcctt
     3121 attgttactg tttaaattgg ctctatttta tataatacaa ttttatcata ataaattcaa
     3181 tcaatacatt taattaatgt acttaaatta ctaaaaggtg ctgtcttaaa atgaaaataa
     3241 atctctattt caaaacagca cctatatatt taaattacta atttagtttt tacatactta
     3301 aatcaatagt tttattcaat ttcataaatt ataatacttc aattataaaa catttctatc
     3361 caacaagcat aaatataaag tgggcacata tacctgcaca taaaccacct atagtatgcg
     3421 aaagtatcgc tttaccagcc aatggtcttg catctaaagc atccatcata cctatgtgtg
     3481 tacttaaata tccactccaa cacataccca ttgctgtgaa tactgctata tcattaggag
     3541 ttatagcgcc tgattttata aactctggta ctaaagatat tgctgctcca acagctccta
     3601 atgcagttac agggaatgct attgcctctg gtgatgtgaa tccaaataat ggttctatta
     3661 tgaagctaat cttatcacca atagcaggta ataattttat accttcatat gcagctcctg
     3721 tgtaaacagc ttgacctgta gcagggtctg tagatggtcc aaaagttaat aacataacta
     3781 atgtacaaac tactaaaact cctggtatta tagccatacc catttcaaca cctaacttac
     3841 caccttcaag tatagcatcc aatgttcttt ggaatacatt accatctctt atttctctaa
     3901 actcttcaag ttttgcacct ttatcattta tttgcttttc agtatcattt ttagggtcat
     3961 aattataata ctttttagta aaagtcaaca taattctaac actaataata ctacctatta
     4021 ttgccccaac attacctatt atagctggta gtacatattc tttaccttga gctatcataa
     4081 aagttgttac aataagtccc ataccaaagg ctgttcctaa attacataaa gcaggtactt
     4141 gatacttctt aaaatattgt gtaaatgttt tgtctttagc aaatggtata atagctggat
     4201 tatcagataa atatgttgca actactcctg ctatactagc accaggtaag ccccatatag
     4261 gtctcataag acccttaaat atcttattaa ctaaagatat tactccaaac tctgaaagca
     4321 atgcactaag tgcacctgca agaactgcca tagccattat taaaaatact gtgtctaata
     4381 aaagcgcatg tgctgtactc attattactt taaacatcat tcctgcaccc atgatgcttc
     4441 ctacatatcc aaatcctacc gccaatagta caataaatac gaaggtttcc atacttactg
     4501 ccttgactct tctcttatcc gacagctttt ccatcttgaa atcttccccc ttttaatttt
     4561 aaattaaggt tcctaacatt ggtacaaatt ttttggtaca aactgagttc taaaacttac
     4621 actcgtaatg caagttgtca gttttttgaa ataaaaaaaa attacgttta tagaataaga
     4681 atatcctctt taatttggat actcttattc tattttatgt tattatatta tcatcctttt
     4741 gtttaaaagt caattttttc tatttttgtt gtttaatttt atttatactc tttttatatt
     4801 tttcattttc ttttatcttt gtaagtactc catataagtt gacttttcta aatacagtag
     4861 ttataaatac caatcctaat gcaattgaac ttgcacacga taatgtactc accccatatt
     4921 ctacagcttt tatataatta ttggtcaaaa cagaatacat agtattatat attccataac
     4981 caggtactaa tattattaaa cagcaaacac ttaatgtagt tgctggagtc atataaattc
     5041 ttgcacataa ctcacagtat atcgcaaaac aaactgtagc tataaaaaaa cataagtttt
     5101 ctgttactcc catatttaaa agaaatttat acgaaaacca agctatactt ccacctattc
     5161 ctgctagtat taagtttttt cctttcatat taaatagcac tccaaagctt aaggcagtaa
     5221 aaaaagctgc tattacttct ataaacaaat gcacttatat cccccctaac ttgaagtaca
     5281 atccaagtac caagaacatt cctactgcta tagatacacc tacaaaaaat acttctgcta
     5341 attttgcaag accagaaatc aactgtccat ctaacaaatc tcttaatgca ttagtcaaag
     5401 caagcccagg aactaagagc attattgctc ctgctattaa atggtctaac gtctttatga
     5461 atccgagctt taaaattaaa attgaacaag aagctataat aactgctcca attgcattta
     5521 caaaaaagtc attaagggaa gatttactta aatacataat aacaaactta gtcagaatcc
     5581 caacagtaaa tgcagctata aattcttcca catttcctcc aaataatatc gaaaaagttg
     5641 ctgctgcaat acctgcaaaa aatattgtta ttaaatccga gtaatagtta tcttcactta
     5701 tttcatctaa aattttctca cataattcaa tatctaaatt atttatactt attgctcttg
     5761 ataatgagtt aacttcatga accaagttta ggtctactct tcttgagctt attcttttaa
     5821 ttgctgaatg taatttccca tctacaaata cagatgccat tacagctgtt ggagttgcaa
     5881 atacatctac gtggtctatt ccaaaagatt gacatattct agctatcgtt tcttcaactc
     5941 tataggtttc tcctccactt tgcaacatag ctttacctgc atttgaggaa aagtttaaaa
     6001 ttctatctat atccacaaaa catatctccc attcttttat agtattattc atattttaat
     6061 acaagatatt atttatttaa actattttaa ataattcata tccaatattt acattttatg
     6121 aaaaaacttg ttttttatgc ttgtcaaaat caaattttaa gcttgtttac accataaaat
     6181 ggctgaatta atatttgaca aacactttca accatttata taaccttcta aattgtataa
     6241 ctttttacta caaataaaat tatatctctt ttaattcttt ctcttgagta tttagcttat
     6301 agtaacttca tatttatata tgtacaatat aaaacattat ttttatgata caaaatataa
     6361 tttgtaaatg tatatatgta ttaactgtgt atatataaat tattcttaac cttatcagtt
     6421 ttttcacata tcttcatcta taccgaccat atattaataa atgatattac atagacttaa
     6481 gtatattaaa aaacttaaat ttagttattt aacaacataa tactctaatt taatctataa
     6541 aaatccatac ctaatataat gcaactctta ttttaacacc atctattaca caaaacaaca
     6601 tattaaaaag taaaatacaa aatatccact ataaacctta ttttaatact gaatatatat
     6661 tataatatat ttaatgtagc ttaaatatag tttaaacata aagaaaggaa acggtatatg
     6721 ctaagaaaag ataaagttct attatctcta cataaattat gtgaaaatat gactttaaaa
     6781 gaccttcaat cagataaaac aggattcact acaatagaaa ttgctaacca ttcatctctt
     6841 gatagaagta ataccagtaa agaattaaat aacctataca atgaaaaaaa agttctgaaa
     6901 atatctggta agccaatttt atttctacat gctagtatta tagaaaattt aattggccgt
     6961 actttgaaca ctgatgaatt ttctataaat aattgtaatg agctacttaa atcaagtaaa
     7021 aatgactcag aagatatttt ttcaacatta cttggacatg attctagtct taaactacct
     7081 atagaaaaag ctaaatctgc tatactctat cctccaaaag gtcttaatac attactcata
     7141 ggacctactg gtgttggaaa aagtacattt gccgaaacaa tgtacaaata tgctgtagaa
     7201 agtaaaatct tttcttctga aagcaaattc gtagtattta actgtgctga atatgcagaa
     7261 aatgctaact tattattaag taatttattt ggttatgtca aaggttcttt tactggtgct
     7321 aataaggata aattcggcat agttgcacag gctgatggag gcatattatt cttagatgaa
     7381 atacacagat taccaccaga aggtcaagag atgctttttc ttttgatgga caaaggcata
     7441 tacagacgat taggagaatc tgacattgtg cataaagcta atgtctttat tgtgtgtgcc
     7501 acaacagaag atatacattc ttctctgctt gggacttttt taagaagaat ccctgttaca
     7561 atcaacctac ctgctttaca ggataggtct ttaaaagaaa gattatctct tattaaatac
     7621 ttctttatta tagaatcaaa atacaccaat gcaactataa aagttcacaa agatgtaatt
     7681 aaatcattac tcctttatga ttgttttggt aatataggtc agcttaaatc tgacatacaa
     7741 cttatgagtg caagagcctt tttaaattac aaaactggac ttaaggataa tatagaaata
     7801 gatttatccc ttgttcctga ttacatctat attggtcttt taaaattgaa tgaaaatcgt
     7861 agtaatctaa ataaattatc agaacttgat tatatgcttt tttatgaatt taatggtaat
     7921 aacttagaag ttgaatatac aaaagaacta tctaaataca atatagatac atcaaacaat
     7981 ggatttaaat attctacaga gattccttat ctggaaaatg atataaggag tatgtttata
     8041 aattacatgg aaaagtactc tacaaaatta ctttcgatat cttcagtaac tcattctgat
     8101 tatacaagta atgagatttt taaagtaatc aatccaaaga tttactatgc tgttgaacat
     8161 tctttaaaag aggctgaaaa aatatcgaga aacaaataca caaaacaaac ttatattgct
     8221 ctttcaatgc atataagtgc acttatagaa aatataaatc aagacacata taatcctaag
     8281 tacaatttat ataatgataa aattttctta gataaagatt taaatatagc taaaatagtt
     8341 ttaagtataa ttgaaaatga cttacaaaca tctttaccta aagatgaagt aaaatttata
     8401 tccatgtttc taaatagcat gtatcttgaa ccaaacaatc aatctgaaag tataggagtc
     8461 ataatacttg ctcatggaaa ttcaacagca agcagtatgt gtgatgtagc aaattcattg
     8521 cttggtacaa accattgtaa tgcaattgac atgcctcttg atgtaaaagt aagtaccata
     8581 cttgataaaa ctatagagtt ggtaaaagaa tgtgacgaag gtaagggtgt acttctttta
     8641 acagatatgg gctctcttat agcttttgga gaacttattt cagaaaaaac aaaaatcaaa
     8701 actcgctcaa ttgaaatggt atctactcca atagttttag aagcagtaag aaaaagtaca
     8761 ctaccagaaa tgactttgga tgaattagct aattccttaa gctctttaaa tccatatatt
     8821 ggacgagcag caatcgattc aacagaaata aactatcctg aaaagtcaag agagtatgta
     8881 ataataacta cttgtataac aggatatggt tcagctatga aagtagcaga ctttataaat
     8941 tcttcactta aaaatattag taaatacaat attaatttag ttcctcataa tatagaaagt
     9001 tttaaaatgt ataagaataa atttagtaat actaaaattc tggcagttgt tggaagtgta
     9061 gattttaatg tagccaacac cccttttata cacatagagg aactactaaa tggatatggc
     9121 ctagatatgc taaacaaaat aatagaaggt acattcaatg ccaatagtat tacaaatata
     9181 aaagaaacaa atctatcata caacagttta ataataaact ttatgaccca aaacttagag
     9241 cttttggact ctgttaaatt attcaactat gtcaattctt ccttatttac aataatagaa
     9301 ttaatagatg aaaacttaaa gagcaaattt aagctaggat atatactaca ttgctctttt
     9361 atgatagaaa gatgttttaa aaatacaact tttccatata ataacattac tgaattaatt
     9421 agtaaaaatc atgaactata tagtttaata cgtgaagcct ttaagccaac agaagcttat
     9481 tttaatattt tgataccaga cgaagaaatt gcttatataa tagacatgat taatgagtat
     9541 ctaaataaat aaaatttata gcacttattt tgtgcatatt aattaatata tgaaatggag
     9601 gacgagattt tgaaaggaaa aataaaacag ttaacaatac ttgcattaat ttttatattt
     9661 ataactcctg tatttgcatt tgcagatact ccaccagtac caaattcttc aagagcagca
     9721 ctacttatag accaggaaac taaaagaata ttatttgaaa aaaacattga tgaaaaaatg
     9781 ccattagcga gtttaagtaa aatgatgacc tttttattgg caatagaagc agttgataaa
     9841 aatcaagtta aagagactga tatggtgaaa atagacaagt ctacagcttc agtaggaggt
     9901 tctacttgta aacttaaaga tggtgatgaa atatctctag gagagcttat gcagggtcta
     9961 atgctagtat ctggaaatga tgctgctata gccatagcta aacatattgg taaaactgaa
    10021 aagaattttg taaatatgat gaacaagaaa gcagaagaaa taggaatgat tgatacgtat
    10081 tattttaatc caaatgggct tcctatatat actgacccag aacataaaga acctcctatt
    10141 gaaaatatgt caactgctca cgatatagtt acacttggaa agtatatgta cgaccattat
    10201 gaaaatcaag ttacaagaat aaccactatg caagtatata atgatactaa aaaggatttt
    10261 acacattata acacaaatcc tctgcttgtt tcagtacctg gtgttgatgg aataaagaca
    10321 ggatatacag ataatgctgg atattgcctt gcattttcta tgatggtacc taaagatgct
    10381 aaaaacgaaa gaaatcatag attgataggt gttgtacttg gtgatggtaa caagaaaaat
    10441 agaatttctt cttccgctac tttattgaaa tatggtaaag ataatttcca ttctaaaaag
    10501 atagcacata agggtgatat tatagagact ccttgtgttg atggtattga tgattttaag
    10561 ataacagtta aggttgataa agatttgtat ggtgttgttt cagataacga gaatatcaat
    10621 cctaaggtag tgtttaaaaa tatgaattat cctatccata agggagatat tgttggtgtt
    10681 gcaaagtatt ataatgactc tggtaaattt gttggaagtg ttgatgtaaa aagtgaaagt
    10741 aatataggat gtattccttt gaaggataag attaagatta aggttgctaa gataaataaa
    10801 aaacttgata ttaaaaattc tgtttgtttt aaagcatagt aaagcatatt attaaaaggg
    10861 tatgttaaat ttataaagac tccactttgt ggagtttttt tgttttttac aaaaaattaa
    10921 aatagtggca acaattgagg atgtgaggga gagttgccac tattttgtat agggtattat
    10981 attatgtttt taagctagtt ttactatctt ctatgtttat ataataactc ttgtgtgtga
    11041 atttaattga taattaatat gaaaattatg tgaagttttt aattgatgta aagtaataga
    11101 ttatatttag acacttaaaa ttataataaa acatcttaat ttttatattt atactaatta
    11161 attctagtat aaatacttat taaatacctt tattcaaaca ttatttcaga tttatactat
    11221 tcttatatgt taaaaggctg tagaaaaatt attttaaaaa tcctacagcc aatactagta
    11281 tcatcaagaa aatttacatc ttattaaact aatttttatt catatttcat tatttatttt
    11341 aatatattaa ataaacttga tagcccagaa gatatttttc ctattgttga tattggatct
    11401 gacacatgtt ttttaacttc aaatgttaca tttgctgctg aatctgtagc agttgtttca
    11461 gatactgctc ctgctttaac tagcacactt acattatcta aatcagtttg tactgcttgg
//
LOCUS       DAEMIK010000137        11405 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEMIK010000137 DAEMIK010000000
VERSION     DAEMIK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.139"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2777)
                     /gene="tcdA"
                     /locus_tag="KPK87_003055"
                     /gene_synonym="toxA"
     CDS             complement(<1..2777)
                     /gene="tcdA"
                     /locus_tag="KPK87_003055"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG2539179.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNRLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSE"
     gene            complement(2994..3176)
                     /locus_tag="KPK87_003056"
     CDS             complement(2994..3176)
                     /locus_tag="KPK87_003056"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539180.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            complement(3505..4005)
                     /gene="tcdE"
                     /locus_tag="KPK87_003057"
     CDS             complement(3505..4005)
                     /gene="tcdE"
                     /locus_tag="KPK87_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG2539181.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            complement(4127..11227)
                     /gene="tcdB"
                     /locus_tag="KPK87_003058"
                     /gene_synonym="toxB"
     CDS             complement(4127..11227)
                     /gene="tcdB"
                     /locus_tag="KPK87_003058"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG2539182.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
BASE COUNT         3749 a         1859 c         1177 g         4620 t
ORIGIN      
        1 tgttcgctat attttgaaaa aatttctttt tctgtttcta cataaactga ctcaccatta
       61 cttttgttaa taaatcttac agagtaagtt gaattatttt ttgagatatc ttcaaaagat
      121 attaaatact tctcatctag attatttaat ttttttaatt catataattc atcagataca
      181 ttttcaagta gattgaactc atctattaaa tcatctatag aattgtgaat tatattttta
      241 acaggctcta atttttcata ataaatgtaa tcaccaatag aagattcaat attaagctta
      301 agattattta aaataaattt tgtatcagga ctaacacttg catcaagtaa taatgttttt
      361 atatcttctg atattgatgc taatcctgga atattcttgg actttgcttt tagcctatta
      421 tctatagaat caaaaaaaat gtattcttta ctagataaat cgctcataat agcttcttct
      481 ttatttatcc atttacctga gtgagccaga agttcttttc ttccctcact attaattctt
      541 acttcatatt gatttgctcc tatagtaata gaatttttat ttacatcagg taaagtggaa
      601 gtaattttgt ccataatact taatagcaac ttcccaggat aagtttcttc aacattaaaa
      661 tcataactaa acatattaca tccaagtaag tttacttcta catttttagg tgatatatct
      721 aattttatgg tatctaaaaa tgaacttatc tcattggaaa gtgaatctac acttaatcta
      781 gcaaattcgc ttgtgttgaa ttcatcttta ccatgtccaa taaaggttac ttttactttt
      841 tccttatttt ttaatctttc aggtatccta tatttattta attctaaaat agattctcca
      901 tcatcactta aaaagtagct ttttgcactt tcattcatat ttcgttgtat aataatacta
      961 tttttaggat ttttagaaaa taaattgcat gttgcttcat aacttatatc atctccttgt
     1021 aactgtatga tataatgaac ataattttta cttccagctt cttctacatt gtttgatgga
     1081 attttattat ttaataaata gtttttgtcg agggcagtat ttttattaaa gtctacccca
     1141 ttgtcttcag aaagagatcc accagtataa tctcttacat atttctcaaa ttgatatttt
     1201 gcacttgctt gatcaaagct ccatagacta tttatttctt gttctgtcaa ttgagataga
     1261 ttattttctg ggaatttaaa ttctattaaa tctgatgctt ttaaagtttt ttctatagta
     1321 ttttcttgta aatttatgaa atcatagtaa gctgacgcat aagctcctgg accacttaaa
     1381 cttattgtgg agcgagcttc tggcataaaa cctacttgta agtatggtgc tatttttgtt
     1441 aaaaacatag agttttctgc ggtagctgaa ttaaataatg aatcatgaaa aattttagta
     1501 gtatctgtga agttattatc agactctatg gctgggttaa ggtgttggtt taaaaattga
     1561 tatctatttt ttacttgttc tattactagg ttagtaagat atgaaccttg ttttgatatc
     1621 aaggcttgat ttataacact gcctaaagcg aaagctattt taatttcaag atcagataca
     1681 tttaaatttt ctaatttaga aaatatctca gatttttcac ttttactttc tataatgagt
     1741 ttaaaattat cttttaattg ttgatcaagt ttatcaaagt tttctgatgt ataattattt
     1801 atatattttt tatacttcat aatagcctct aattttatca tttcccaacg gtctagtcca
     1861 atagagctag gtctagatat tgttttaaat aaatcagagt gaatacctgg aagcatatca
     1921 acatctaaat atactccgcc aaaatttttt agggctaata atcttactat gtcagatgct
     1981 gcagctaaat ttccacgatt taacaactcc tgactataaa tatttaataa ctcttgttct
     2041 gtaaacaaac tattagccct gatatctatc ccatgattac tatttatttt tctcaaagaa
     2101 tttgttctat atgattctaa tacagtttca tctctattat attcagatac tagatgagac
     2161 tttataatat catctattgt aggtactgta ggtttattga tttgagattt ataataattt
     2221 ataaaccttt tttgtctatc atatataaat tccatccttt ttttgtaaaa tttcatatta
     2281 tcaaattgag gattttgaat ctcttcctct agtagctgta atgcttcagt ggtagaagat
     2341 tcaactatag ccttttttag tgtatttact aagaatgctt cactatcata ccacagttta
     2401 atattatatt ctgcattaat atcagcccat tgttttatgt attcaagagc aatatcactg
     2461 acttctccac ctatccatac aaaatgtaaa tttttttcta cagggcttgt attggaattt
     2521 ttaataagaa ttacttcttt taatatatct ttttttagat tagagagtgc tctatttctg
     2581 cttgaagttt tatatttatt cataaaaaca tcaattgatt catttagttt ttttaattgt
     2641 aaatatttat tttcattatt gtttgtagtt aacttattat attcgtctaa attagttagt
     2701 atagttttat actcattttc tcttggtcta atgctatatg cgagttttat taactcttct
     2761 ttagatatta aagacataaa aacctcctag tattattatt tttgataata aatccacttc
     2821 tataaccaca tttattcctt ctgaaattta aattgatttt cttgttttta aactttgaaa
     2881 ataaatctta tacactcctt tctatttttt aaaaataaat taatattgat tttttataat
     2941 ttaagttttc atatttaaat cacttcctaa catttcaatt taaagatact caattagtta
     3001 attgactctt ctattagata aggagattgt ctgatagtaa tttgtaaaca tatcttatat
     3061 ttttttaatg tttttatttc tttatatatt tttcctataa cttttaaact atcaattaaa
     3121 tcatttatta aggtttgata cttattttct atatcatttt gtccttcatc acgtattttt
     3181 gtgtttttga ataatttcca tcacaattta cacatatttc tatgtttaac tattatagtt
     3241 tgtgatatct gtaactaatg tagcttaata gattttacca actgaccatg caccatcttt
     3301 gtgaaccaat gtttgatata ctaccttgtc atctatatag taatgaacac ttgcttcgaa
     3361 attatatgta tttaatactt gtgtgtctct tggcatctgc tccctcgttt tcattattag
     3421 tttcatgaat tatgataact ttcagctatt cttatttgga taacactttt tatttgttaa
     3481 tatctttatt atatctacca ttacttactt ttcatcctta gcattcattt catctgtcat
     3541 tgcatctagt aaaattgcta ttttttcctt taatctctta ggtactggta atccacataa
     3601 gcacatattt tttaatatac ttacactttc atataaaata aataaaatag agaaaaattc
     3661 agatattcca agatgtttta gtcttaaaaa attgatacaa tcttgtggta acataaataa
     3721 aaagttaaac tttgtaagaa tgtcaactac tgataaaaaa aatatacatg ctatcattgc
     3781 tacttttctg attcctccat ctattccaaa actagaatta aatttacgac tttttattgc
     3841 acttaaacat cctaataatg tatcaaatga tatagttaaa attactaact ttataaatat
     3901 atgctctgat aaaaaagata ttgtcatatt cataacgcct cctaggttta tataaaaaaa
     3961 tattttgtta ccattagaaa tataaaaagg tgaactactg tgcattcatc atagtccacc
     4021 tttttattgg taaatcttca tattagatag aacactttat gtagtgtatc attttgttta
     4081 tttttatata tttaagtata agaggatata tttaacatat ttttatctat tcactaatca
     4141 ctaattgagc tgtatcagga tcaaaataat actcctcacc atcaataata actgaaccag
     4201 ttgctgcaat atattcatct gtaaaataat atctcttttc atctaaatct aaccaaccag
     4261 tatagtttat tgattctccc tcaaaattct catccaaagt attttgatgt gcaaagtatt
     4321 taaatccatc tggtgtatta aatactccaa tctgcatgac accatcttca ccaaaataga
     4381 acatcttatc ttctatattt atataaccaa attgcatttc accattctca ttaaagtaat
     4441 aattattatt ttcaaatgat ataagacccg ttctcattat gcccttctca tcaaaataat
     4501 attttatatc atcaattaaa ttaatacctt tgcatgcttt tttagtttct ggattgaaat
     4561 aatatttatc actttcattt tccatatcat atatccatcc agtctcaatt gtatatgttt
     4621 ctccaaaata atatacatct tccccaactc taactaaacc actatattca actgcttgtc
     4681 cgtaaatatt atcatttaca gtattagcag gtgcaaaata tttatatcca tctgaagtat
     4741 caaatacacc aatttgaact ataccattat catctatata gaaataattg tcatctatgt
     4801 tttgtactcc agattctata attccagagt cagagaagta gaagacttta tcatttatag
     4861 tgacaaatcc aacttgcata attccatcat cattaaaata atattgacca tcatttatta
     4921 atgacaaacc tatatatgct tctgctgtat cttcatcaaa ataatacttt gaaccatcct
     4981 ctaaatcttt ccatccaact acagctgtaa atgaatcatc aaaatagtaa attttattat
     5041 tgaaatttaa tataccagaa tatgagattt cttcaccttc ttcatttcct aaatcttcat
     5101 tatgatgagc aaaatattta aatccatctt ctgtattaaa tactcctatt tgcatttctc
     5161 cgttttcagc aaagtagaaa tgcttgccat ctatttcagt gtaacctact ttcataacac
     5221 cagaatcatc aaaatagtgt ttattatcat ttatactaac aaatcctttt tgcataactc
     5281 catcagaatt gaaatagtat ttatcatcac ctatttgatt tagaccttta aaagctttac
     5341 ctgtttctgg gctaaaatag tgcatttcac catctaattc tttccattct acagctcctc
     5401 tataattatc atcaaaataa taaatatttt cgtcaataat taattttcca gtaaaatcaa
     5461 ttgcttctcc ttctaggttt tcatcaagtg tattagctgg ggcaaaatat ttaaatccat
     5521 cttctgtact aaatacacct gtttgtaaca ctccactttg gttgaaataa taatttttgt
     5581 catcaattat tgtctctcca attgaagcag ctccaccatt aattggatta aagtagtatt
     5641 tatcatcgcc tacagtcaca aatccagtta tcaaattatt tactggtggt ttaaaataat
     5701 ataatgaatc attaatatat attaatccag ataccatcat tccaaagtta ttaatataaa
     5761 atttctcatt atataaagaa actagaccca aatcatagcc aatcaatcca tcctcataat
     5821 atgaaggtgt aaatgataag attatttcac ttacaggtac atcttgttta tcactaaagt
     5881 taaaagatag cttatttgcc aaagtcttat ctttaaaaac attaacgaat cttattttaa
     5941 cttgtgacac cttattttct tcactagttg acataagaat aaaatcatta ccatcattac
     6001 tccatacata tcgtatagat agatcattga tattaacatt tattttttca tttatataat
     6061 ttgcatctaa tacaataact tctggataag tattatttgt ttcatataca ggcgttatat
     6121 ttatttcatc tgtataaata tttggtgaaa ttacaacttt attaacacaa ctgtctattc
     6181 cataaagata cttttgagag aaattgataa cagttgaaga tatatctcca gaatcatcta
     6241 aatcataatt tggttccact atcatatttt gtctatttcc tacatataaa gtataattag
     6301 tttctagtgt attaaactta atgaaatatg gttgtatatt atcattttca tcacaaataa
     6361 actcaaattg gccaatagaa gtagtaccac ttattataaa attagcatct aatataaact
     6421 taatattaga ttgtaagaaa ttaacgaaaa tactttttat attcatactt tctaaaaagc
     6481 tcattaaaga atctgaagta tttgtattac cttttctatt catgaacttc aaaatctcag
     6541 ctactccact ttcatctaaa tgcacactat ttaactttat agttttttca tcttgtagag
     6601 tcaaagaaag agagattttt atatcatcct taagataata acctgttaat atattaacat
     6661 tatctttagt tataactttg acatctttca aattattact ataatatcca aatgaaggct
     6721 tactatcatc catataaacc ttacttataa gaactacatc aggtaattca gatacaaata
     6781 aaccttcttt tgttgaacca ttaataaaac cattctcttt tccttcacta tctacaaagc
     6841 tatatggtat atttttctgt aattcgctat tgaatcctat ataatctatt ttctgttgaa
     6901 tatgatttga atttaacatc aatattttta attcgccaga aataagtaat ttatatgatt
     6961 tagataataa atcaacttct ataattgcat ttattccttc taaaattgaa aatgttaaag
     7021 aaacaaatcc attacttcca tttacctcac cagaaaaatt aatctcatgg ctatttaaga
     7081 taattttatt ctcttcaata cttagtgtag ataaaatacc ttctattaaa tcaccttttt
     7141 taattttatc agattctata gttacatctc tcacaacatt atcaacatct ataatccaaa
     7201 catcactttc acttaattct atatttatac ccatattata ttgagaaaga gacaatgcat
     7261 aagttcctcc tgaaccatag aaagaatatg ataatttttc tcttatatat tctgtagtta
     7321 ttattggaac tataaaactt ctagtattac tatctaaatt tattcttata ttagtatctt
     7381 catatcttgg ttttaatgtt gttattaaag catcagctat aaaagcaaaa tatctccaat
     7441 aaaactcacc ttcatagtta tctcttatac ggtctaacag ttttgtgcca tcattttcta
     7501 agcttcttaa acctggtgtc catcctgttt cccaagcaaa tactctattt ggagcattag
     7561 gtaataccat taaatctttt gacaaatcaa gttcttcttt ttgtacttcc aatacgtcat
     7621 atatagataa gtgtggctct ctatatgtta ttgatggtgc tgaaaagaag tgatctatat
     7681 catcagttac agtatgacct gaaccacctt ccattctcca gatttcacat ttacctaaaa
     7741 ctattgaatt attattaaaa tctatttctg atatcactaa atcatcttgt ggcatcatta
     7801 ttttatcatc taataaagta aatactcctt cagtttcaac taatgaaaca tgtttaaaat
     7861 aatctacaac ctttgttgcc ttatctcgaa gtacaagttc attgtttact aagcttggta
     7921 tacctgctga aattcctgct aaaggaacta aaagtatact aaatccacta gctatcccca
     7981 aagatgaagt aatgattgca gttgtagctg ttgttaaatt tactgccatt atacctatct
     8041 tagcttctat ttcttgtctt aataatgggt cactcgtttc acttagctct ttgattgctg
     8101 cacctaaact tacaccatct ataatagttg caattatagg taatccttca gataatgtag
     8161 gaagtaagtc tatagtttca tctaatgcag ttgatactaa ttcaacaact ttggctgcat
     8221 ctgtaatagt atttaaacca gtactaaata attgagcgta aacttggact ttcattgcta
     8281 cacttaaatt actaagagat tctttagaac tattatattc tattaatgat tgtataaaaa
     8341 atgcagcatt taaagtattt acttcgtgtg tagtatctaa atttactttt tttactaact
     8401 taccatttac agtatcaaat atagtacctt ttatcttaga aatctcttca gttatatgat
     8461 tagcatattc agagaatatt gttttttcag tttctacaaa tatagattct ccagtttctt
     8521 tattaataaa tcttatacta aatccctcat cagtctctga tatgtcctca aaagatataa
     8581 aatgagaatc ttctaattca ttctgttgtt ttaagtcaca tagtgcatca gaaatagatt
     8641 ctattagttt aaattcatct tttatataat taatagagtc agaagttaaa ttcttagctt
     8701 cttcaatcct ttcctcaaca atttgcgtat ctatatttga aataacattt atctcacatt
     8761 ctgttaacat tactttttct tctagttcaa tatcacttga attagaatta tttctaattt
     8821 cttgtaataa tgtagatagc tcaggtaaat ttttagattt tactgtaatt ttattttctt
     8881 taggattaaa tgatatatat tcttttgatg aaatatcctt tataatactt tcttctttat
     8941 ttatccattc accagaatga tccaataatt ctcttcttcc ttcactattt attctaactt
     9001 catattgatt tgcacttact ataatagagt cttgacttat agatggcatt aattctgata
     9061 ttttatcttt aactttaagt aataattttc caggataagt ctcctctacg ttgatagagt
     9121 agctaaacat attacatcct aataaattta tttctattga cttaggagaa atatcctctt
     9181 tagctaaatc tattgctgct tctatttctg tggataatga atctacatca aaacctgcaa
     9241 atatatcagt attaaattca tctttaccat gaccaataaa tgttaattta atcttaggtc
     9301 tatcagaaat tatacttgga attttatact tgtctatttc ttgtatttca ccatctccag
     9361 gattataata atatgcaatt tctgaatctt ctatattttt ctgaaacagt acactatcat
     9421 aaggagtctt tgcaaataag ttacatgctg cttcataact aattttatct ccttgtaact
     9481 gaacaatata gtgtatatat cctctctctg aacttcttgc taatgaagat attttttcta
     9541 aaagatactc cttgtcaact actatatttt gagaaaaatc aagattatca tcttcaccaa
     9601 gagaaccttc aaaataattc cttttatatt cttcaaattg agctttagct cttgcatcgt
     9661 caaatgacca taagctagcc atttcttgtt cagttgattg agaaatatta gttttagaga
     9721 tttcaaagtt tcttaaatca gcttctatca aatggatatt catactgcct tctttaaaca
     9781 ttaataaatc ttgataagct gccgcatatg cttcagggcc acttaagtta atagtagttt
     9841 taacatctgg gaagaaacca actcttaaat actttcctag ttccatcata aatctaccat
     9901 tatctgcatt agcttcagcc attatactat caataaaggt attcgttgta gtattaaaat
     9961 cattatcctc gctaatagct ggatttaaac tattattcaa tattttatat ctattctcga
    10021 tttgttttac tattaaattg ctacaatatg agtctttcac agaaattagc ccttgattta
    10081 taataccctt actattaaat gcaattttaa cttctagtgg tgatgcctcc atatcaccaa
    10141 gtgatgagaa tatttctgat ttatctgact tagaagctag aacagattca aaactacttt
    10201 gaacttcttc gtctaacatg tcaaaatgtt ctgaggtata ttctggtata tattctttgt
    10261 atttcattat agcttctaac tttgtcattt cccaaaaatc cactgttact gaactaggtt
    10321 tctctataga ctcaaataag tctggttgta ttcctggtaa catatcaaca tctaaataca
    10381 taccaccaat ttcttttaat gcagatattc ttaatatgtc agaagcagca gctaaattcc
    10441 acctttctac caactcttgt tcatataagt tgaatgactc tccattttta aattcttcaa
    10501 agtttctaac atcatttcca ctattctgtg taattttatt taaggattct tcaatatagg
    10561 tattaagttc atctatctcc tttgaatact catttgaaag atatgtcttt acaatatcat
    10621 caattataag ttcaggattt tcttctcttt gagctttata gtagtttatg aaatttttct
    10681 gtttatcata aattatttcc atacgttttc tgaagaattt attatagtca aatctagggt
    10741 catttaagtt ttctctaaat gattcaagtg tatcatttat tgctgattct actacagttt
    10801 ttttcaatgt gtttatcaaa aatgcattac tatcataaaa aacattaaca ttataatcac
    10861 tatttacatc tttccattga tttatataat taatagcagt gtcatttatt tgacctccaa
    10921 tccaaacaaa atgtaaattt ttctcaactg gagttaaatt attattcttt agctctaata
    10981 cttctgtaac tagatattcc ttaaattttt ttaaggcttt atttctacca gattttttat
    11041 atgtatctat ataaatatct gttaaactat ttatatcttt taattttaaa tatttttcga
    11101 ctacagtatt ctctgacata ttatgatatt cttctaaagc atccaatatt gcaacatatt
    11161 catcttcttg agtacgaaat cttacatttg ccattttttc taactgtttt ctattaacta
    11221 aactcataaa attttctcct ttactataat atttttacat ctaaatgcta aaactctttt
    11281 atatatcctc ctttctattt ataaaatata ctaatatcta cttaggtttt catatacatt
    11341 cacttcctaa cattttaaat ttaagacact cagttgatta atttgctctt cgattgaata
    11401 aagac
//
LOCUS       DAEMIK010000138        11303 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEMIK010000138 DAEMIK010000000
VERSION     DAEMIK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.140"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            256..1431
                     /locus_tag="KPK87_003059"
     CDS             256..1431
                     /locus_tag="KPK87_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2539183.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1604..3001)
                     /gene="asnS"
                     /locus_tag="KPK87_003060"
     CDS             complement(1604..3001)
                     /gene="asnS"
                     /locus_tag="KPK87_003060"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG2539184.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3078..3311)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3480..3674)
                     /locus_tag="KPK87_003061"
     CDS             complement(3480..3674)
                     /locus_tag="KPK87_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG2539185.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3947..5620)
                     /locus_tag="KPK87_003062"
     CDS             complement(3947..5620)
                     /locus_tag="KPK87_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG2539186.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5630..9028)
                     /locus_tag="KPK87_003063"
     CDS             complement(5630..9028)
                     /locus_tag="KPK87_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2539187.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDMGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9195..10391)
                     /locus_tag="KPK87_003064"
     CDS             complement(9195..10391)
                     /locus_tag="KPK87_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG2539188.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10412..11233)
                     /locus_tag="KPK87_003065"
     CDS             complement(10412..11233)
                     /locus_tag="KPK87_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG2539189.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3599 a         2068 c         1307 g         4329 t
ORIGIN      
        1 tttatatttt attatgctta atttgaattt attctattct ttattttcag tagtaaacta
       61 tctgtaaatc ttaattataa taaagtatta atcaataaag ttactcgaca ttatctaata
      121 aattttatct atcacaattt tagattgtta cacacaatat attttgatat aattaaatca
      181 taaacgaagt attccaaata tctaatgata gaaaggaata cttattttta tgctttattt
      241 ttaatggagg cttatatgaa cttagaaaaa ataaaaaaat tttttggatt tcctctagac
      301 aaaaaacaca aagacaatgt tgagaaagaa ctctgcaatt atctttatca tacgggtaaa
      361 atcatttgca taataatagt aatatctcaa ataattatga tgatatcaat ctcagctaga
      421 gaaggaggcc cttttgtcac accaagacgc caatggtatt tttatatgta tgtaacacta
      481 ttttgtgtat cttctgtctt tatgtttgta ttttcttatc tttggaataa aaaaaaggat
      541 tgctgtacgt tttatataaa tttatggata atttatgtga cttttttctg cctatggggt
      601 tgtgtaatta ctctacttga ccagttaggt ggaaatgacc taagtgtatt tagttatatt
      661 atgttagcag ctgcagcttt ctcagtatta aaatttggag aatcagtaat agtttttgga
      721 ggatgctttt tgtttcttaa tttattctta cctgggattc aaactatacc aggcaatgag
      781 tttagtaata ttgtcaacac attttttata acatcccttg ccatttttgt ttctacaatg
      841 ctatatagaa atcgtgtatt tacctactat gataaacttt taattagtca acaatatgaa
      901 gaaatatctc atataaataa tttacttaac gacaaagtta tgacagatga ccttactaaa
      961 atgaacaacc gtagatacct tgaagatatt ctacaaaaaa aattaatcag tcatgcaaat
     1021 caagaaccta taactggaat gatgatagat atagattatt ttaaacaatt taatgataat
     1081 tatggacatc aaaagggaga ctattgtcta caaaatattg ccaaagtaat tttagatttt
     1141 acaagttctg aaaatgaaca tgctatacga tatggtggtg aggaattttt tatatgtatt
     1201 tttaactgta atgaggcaga tgctaaactt aaatcagaat gtttacgaaa aattatagag
     1261 tcctgcaatt ttgagcgtga tgatgttcct ttaaaatgtg taactgttag tattggagtt
     1321 tatacaaaga aaattggaaa gacaataaca ttaaatgagt ttataagtaa ttgtgataag
     1381 gctctatata ctgcaaaaga acaaggacgt aattgtgttg cattttatta aaaggttaca
     1441 tactattaca taaacctata agtattatat cgcaaaataa aagtaatcaa attatatatt
     1501 tatagtacaa taaaaataac ttgcatatct ctaaaataga taaatatgca agtcatttta
     1561 gtgtttatac ttaaagttat ataagtttta ttatatataa ttattaaaat tcagcatttt
     1621 taggagttct tgggaaaggt ataacgtctc ttatgttaga catacctgtc atatacataa
     1681 ttattctttc aaatccaaga ccaaatccag agtgagtagc agtaccaaac tttctaagtt
     1741 ctaaatacca ccaataatct tcttctctta atcccatttc attaattcta tctaacaata
     1801 catcttctct ttcttctctt tgagaacctc ctacgatttc tccaactcca ggaactagta
     1861 aatccattgc tctaacagtt ttaccatctt cattcattct catatagaaa gcttttatat
     1921 cttttggata atctgtaaca aacactggac aattatatat ttgctccgtt atatatcttt
     1981 catgctcagt ttgaaggtca catccccact caacaggata ctcaaattca tgcccagatt
     2041 caattaataa ttccactgct ttagtatacg ttattctaat aaactcagag tttactatct
     2101 tatttaacct ttctaacaat cctttatcta taaaactatt aaaaaattcc atttcttcag
     2161 gagcattttc taatacatag cttataacat attttatcat atcttctgca agttccatgt
     2221 tatcttcaag gtctgcaaaa actatttctg gctctatcat ccaaaattct gaagcatgtc
     2281 ttcctgtata agagttttca gctctaaatg taggtccaaa agtataaacg tttctaaaag
     2341 aaagtgccat tatctcggca tttaattgac cactaactgt taaattagcc tcttttccaa
     2401 agaaatcttg agtatagtct attttgccat cttcagtttt tggaatatca tttaaatcca
     2461 tagttgttat tctaaacatc tctcctgcac cttcacagtc actacctgtt atgattggag
     2521 aatgtgcata tacaaagttt ctttcttgga agaatttgtg tattgcatag gcagctattg
     2581 atcttactct aaatacagct gaaaaagtat tacttcttgg tcttaaatgt gctatcgttc
     2641 ttaaatattc aagcgtatgt ctcttctttt gtagtggata cgaactatct gattcccctt
     2701 ctacaactat gctttttgca tgtatttcga ctggttgttt agccccttct gtagatacta
     2761 actctccctc tacaagtata gatgagctta ttgctaattt tcctacctct ttaaaatttt
     2821 ctactttctc atcaaatact atctgcatat ttttaaagaa gcttccatca tttagctcta
     2881 taaatccaaa atttttagat actctagatg ttcttatcca tccagctact tttacagtct
     2941 tccctatgta ctgttctttt tctctgtaca attcttttac tgttataaat tccttttgca
     3001 ttttaaatcc tcctagtata attgttatta atttctttta aatataattt caataaaaaa
     3061 agcccttcat ccctatataa agggacgaaa gactattact ctcgcggtac caccctagtt
     3121 gtcataatga cctcttagtt aagcaatgct taaagcctat aacggtgcct tccgtctaag
     3181 tctacttact aaataagctt tcgattagaa actccaagat gttcttcaat ataaacctcg
     3241 tattgagctt tcaccatcct caactcgcta gaactacatt ttatatctac tcttctcttc
     3301 acagtattta tcttttatta tttctgctaa ttaacaaaat tataccaatt tataaatttt
     3361 tagtcaataa atttatataa ttgatactga ttgttattta aaatataata attctacctt
     3421 acataatgat aataaatact tatcttcatt acttctttat tacttcaatt tccaaaattt
     3481 tataatttac ttccattgtc aacccagtct tttgcttctt cgacaccctt aattgaaggt
     3541 attgaaactt tactatgagg ttttaatttt tcaacatcag cccatgctgc tgtcgactca
     3601 tttccaacta aaagtgaact tttaagtctt ttattatctt taccttttaa tgctcttgcc
     3661 tttttacttt ccaaaactat cacctctatt atttattttg taatagtttt agcaatttta
     3721 caaataaaat tcataagtct aaattataat gatttttaaa ttaacatcat atcttttcaa
     3781 tacttaattt atacctattt atatacaaaa tatttacttt gtgtattcaa aatataaaac
     3841 tattttttaa ttttcatatt tattttttat gctatttttc tactctatct aaagtatttg
     3901 atacaattaa actaataatc aattataatt ttacataggt tcttatctat agagtatttg
     3961 ctatctgttg aatcgtattt tttaaattta aaatcccata tccctgcgat acatttggat
     4021 atgtatatat ctcaagtttt gtagcaccca atattaaata tgtttttaaa acttgtgtaa
     4081 acaatgataa tctcggtaca ttatcctgtt tttctaagta ttccatcaat aaggctaaaa
     4141 cacctgtgac tatagaactg ctcactcctg ttcctgttcc tgtattatat gttccatttt
     4201 tatatgtgga tattatatct acacctgaag ctactatatc tggcttaatt cctcttcctc
     4261 taattggtcc tttcgaagaa ccaatccaca tactatcagt cttattgtta aatgtaccaa
     4321 ctgtaattac atcatcacta gcagcataca tagttatagt agctactgaa tcaggttcta
     4381 aaaaccttgt gtctggagct atcaaatttt tatttggcaa gtatatgtca tattctccat
     4441 ttattataag ttctgaagtc aatcttaaaa tccatactcc aggttttata tctcttaatc
     4501 ttatctccaa attttctttt ccagaggtaa tatatggata aataaatctc attgcataag
     4561 tagtattttc taagttaaat tttcctctat atatataaaa gtctggagaa taccttatat
     4621 catgactaac ttcacctgaa ggagatatta tctgtgctcc cactttatct ggaccattcg
     4681 tatttagtgt tatatcaaga gcatagtcat ctccatcttg tattattaca tcttgaactt
     4741 ctcccacact gctaaatctg ccagaataat gaatatctgt attcccttga tttcctgcac
     4801 cactcactac taccacacca gcactactca atatattaaa agtatctagt attgaagtag
     4861 ttgctacagc acttgactta actccaatag tcaaatttat tatcagtggt ttattttctg
     4921 ttcttgctat gttagtaaca tacgttatag ctgctaaaaa atctgaaaca ctgtaattta
     4981 tccttccagc ataataagta tctgtatatg attttaattt aactactatt aagtctgatt
     5041 ctgtagtaat acctctgtac tgagaattta tccttccatt tccaactaat ataccagaaa
     5101 ctaaagttcc agtacctata ttatcttgac tcaaactccc atcatttcta tttatggcta
     5161 tatttaactc acttcttgta aattcacttc caaaaataaa accttctgga ggtggatttg
     5221 tatttgcttc ttggtcccat aaatataaaa ctttactagt tccatcatca tttataaaat
     5281 caggatgcaa ataatctatc ccagaatcta taactgctaa taaaattcct ctccctgtta
     5341 tatcattata tggattttca taaatataat cagtttcagc tgctgttgta atggtttctc
     5401 cattatttac attattagtt atttcaatta gactgctcat tggttcagat tcttcccacc
     5461 aagcaacttg tattatatta tttaaaatag tttcatcaaa atctaaaggt acatatatta
     5521 ctgcaagctg actatttaaa accatatacc tatttattcc attttcttgt agggcagatt
     5581 ctatatcacc ttgataaatt atacaataag atttttccat aaatccctcc tatcttaaaa
     5641 catcaaacat tcctctaaca tccaagagcc catatccaga atttgtattt ggatatactg
     5701 tgtttgaatc ttttctagca ccagcttgca taaaagtttt tattttttgc acatatgctt
     5761 ggtttggata tctaccgtca acaaaagtat attgaaaata cattgcagca gcaccagctg
     5821 catgtgcact tgctgctgct gtaccagtta tagttgcata cgtgttccca ggataagcag
     5881 ctattatatt aacacctggt gctactatat ctggttttaa cctgtcttca atagttggcc
     5941 ctcttgatga actttgccat aaactaccat taatagtatt gtaggctcct atggttatta
     6001 aatcatcttg aatcgcagga taatttatag tgtaaaaagg gtctacctct ctaaatctag
     6061 ttccactttt taaaagttct ctatttggaa ggtataagtt atatcttcct gttataatat
     6121 aaactcctac taatcgaatc ttccatacac ctctttttgc attctttaaa gtaacattag
     6181 taaattgctg tccagaaaaa gttgttgggt atatatatgt tatagaatat tcagtcccct
     6241 ctaaatcaaa taagcctgtt actttattat aatttgaaat tccaacactt ttactttctt
     6301 caccagtggg agatactatt attacatcag ctttatctgg tctatttaac caaagttcta
     6361 atgataattc ttcctcgtct tcatttaact ctaattcaac ttcaacagaa cctccaacat
     6421 atggtattat tccagatgta tgtgtctgtg tattaccctc atttcctgct cctgccgtta
     6481 tacataaccc tctagtaaag aacgcttttt cactattact cctattggtt aaacctgcta
     6541 ggctactggt acctaaagac atatttataa ctaatggtct tccaagttca aaagcttttt
     6601 tatatgcata ctgagatgca gcaaacaaca ttgcactatt ataaaaatca tctatcttgc
     6661 cgagttttat tattattagc tcagaatctt ctgctatacc tgcatattca ctatttacat
     6721 ttccaagccc tgcacatatc ccactcaaca tagttccttg accaacttca tcctgagata
     6781 gactagggtc attttctgcg atagctcgat ttatatcttc tcgtgtatat tctgtcccaa
     6841 tataaaaacc atcaggagga gttccttctt ttgtttggtc ccataaatat actatttttg
     6901 aagtgccatc tggataaata aaatctgggt gtagatagtc tattcctgta tctgcaattg
     6961 atattagagt ccctcttcct gttatattaa tattaggatt atttttaaag aaatttacgc
     7021 ccatttcttc tgttgcaact acacctccaa aagtacctcg attaatttct ccaagtaatg
     7081 tcatctttgt agtcgataca gttcttatga ttgaaggaag ttgcaaaact cgttgtatac
     7141 tactatagtc tgtattattt atagatagta cacctaatga atcacttatc ttaaaaaacc
     7201 tttgactcat acctaaccca gctagttctt cttcaaattc aggagtatgg actatatcta
     7261 ttcctgatag tattcctgat tgtctacttg catttttact tatataaatt tcattctcta
     7321 agtcattatg tatttgcata ttataaccat ctataaactt ataactattt tcctcatctt
     7381 taaatgactt attaatactt ctatatccca tatcctctgt ttctaaatct tgatttatat
     7441 tagatagagt gtacaaatct atatctgata aatttaaaaa accaaatcct gatgaattat
     7501 ttggataaga ttgattactt aatctccttg ctccctttag aagtaatgcc cttaatttct
     7561 gtgaatacaa aaataaatca tttccattta caattcccca ttccataaac aatgaacaaa
     7621 cccctgtaac atgaggtgta gccatgctag ttccagttaa tgctccactt gtacctcctg
     7681 gtaaaaaaga tactatatct tcacctggag ctaataaatc aggtttaaaa acacctaatt
     7741 gtgtatctcc ttcaccagaa aatatagata ctatatctgt tcttgaatta aaactaccta
     7801 ctgttataac tctacttgct gttccaggaa ctgttacagt aagctcttga gtaggaatca
     7861 gaaatctagt atttctattt agaccttcag aagttggcaa atatatattt acattaccag
     7921 ttactatatc tattggttca aatacaattt tccataaccc aggagttatc tgtgtatttg
     7981 aacttaattg tagagtaact cttcttgtga gagaatacgg tgctataggg taaaaataac
     8041 cagttattct agtttcaccc aaagtattcc ttatctcacc tgaagttaga gagattgctt
     8101 gtgtttgatt atttgatggg tttaccaaat gtacactaaa atcatcaaca aaatctggcc
     8161 atatatttat gtttaatata cgctctcctt ccccaactat aaactcaact tcttctgtaa
     8221 tattattttg aagcctaatc cttttatgac ctcctttatc tgcattattt ccagctgcta
     8281 caactatatt gtttttccaa aataaacaca tatcatctat atattgttca aataaagatg
     8341 ttcctctatg tgaaccttca ttacttccat aacttatatt taatgtaaca ggcattctta
     8401 attctaatgc cctatctaaa ataaatttaa ttgccctcat aaactctgta cttctcgaaa
     8461 aaatatcggt ctgtatattt ccaactctaa ccactattat tctggcatca cttgcaatag
     8521 ttgcacaaat acctgcaaca tgtgtcccat gtaagctagt tgttgaaata ggtatataca
     8581 tacttccatc tattgcatta tttatatctt cattagtata taatgttcct tctctaaaac
     8641 cttctggtgg atttccttga atagattggt cccaataata taatatctta gaccttccat
     8701 cactatctct aaacacaggt agagtataat ctattcccga atcaattata ccaattatag
     8761 ttccttttcc agttaaacca gttctatttt taaatcctgt tattcctgta cttgaaaaac
     8821 tttgcacatc ctgagtctgt aatataaaag gcttttctat aaattcaatt tctggataag
     8881 ttaaaagtat atttacatct tcttcattac ttgatgttat tattgcatat gatgaattaa
     8941 gtatctctac tgatacacct aattcttctt ctaacctcaa tatatctcca ttgtatttta
     9001 caattaattc ataatttatt ataatcacct cgacttctaa aattattatt aatgtatatg
     9061 ttttgtgtga gtctaaaatg tattagacta tataattttt taagtttcag ttatacaatt
     9121 tataatagat atattaaaaa aataaataat gatggtacaa ttttgaataa atcataccat
     9181 catttatatt tactttatct tgaaaccatt aaatttttca atgctttctc aagacctttt
     9241 acagtcaatg gaaacatatt aaattcactt tgtatcattc tttgagtttg ataccaataa
     9301 ttaattgaat cccatccatc tctaagttct ggattcatcc atacaatttt actgtacttt
     9361 ctcttaaacc ttttaagcca ctctatacca ggtgtgtaat tatatggacc acgatagtta
     9421 ccaccaatat gaagcaactc agaaggcgac atagaagcat ctccaacaat tataacttta
     9481 tagtttttat ctatattttt taatatccat tcagtattaa tagatttact taaccaacat
     9541 tcaggtgttg taaataatct atcataaata cagttatgga aataatatat ctttacatct
     9601 ttaaaatgat ttgatttact tacagactga aataatgtat tacataaagt gctataccct
     9661 ctcattgaac cacctgaatc cataagcaat aaaagcttca ctgtattttt tcttggcttc
     9721 tcaaaaaata actttaaata tcctgcatta ttacaagtct cttcaatagt cttatccaaa
     9781 tcaagctctg tctttggagc atctattcta gtagaaaatt gacgaagtct ctttagtgca
     9841 acttgaaatt gacgcatatt taaaacttca tcatctctaa aatctctgta ttttctttct
     9901 cctgcaactt caagaacagc tctaccataa gtagaatttc cacctatacg tataccagtt
     9961 tgcccttttc ctgaatgtcc taactctgaa gtgcctcctg tgcctatcca ataattacca
    10021 ccattatgag gagaatcttg ttcttttatt cgttcctcca atttagctct taatttatca
    10081 agatttatat ttggattttg ttctaatctt tcaaactctt ctaaatctat atctggtttt
    10141 ttaagccaat tcataatctc ttctggaatt tcttcttggt gttctatacc tttaaaatac
    10201 tccaaaaaca ctgcatcaaa cttatcaaaa tcactttcac tttttattaa aatcatcctg
    10261 caaagatagt aaaaatttga aaagtttgaa tagcacagtc ctttatccaa tgcttcaatt
    10321 aaagtcatcc attcatttaa agatacattt aatcctcttg acctcaataa ataaaaaaat
    10381 tcaataaaca taattatcca gctcctatta actataagtg tctttgcata gattcaatat
    10441 cctcattatt cttaagtagt attcctgcaa aaggaacttc tttttctatt ttttcaatag
    10501 gcattccact caaagttaat gcttgtatcc agtctattaa ctctgatgta ctaggctttt
    10561 tttgtatgtc ctttaaactt ctaatccaat aaaaagtagt cattacttgt tccaataggt
    10621 gttcttctac tttatcaaaa tgtactttaa ctatttcttc catcatgtct ctatcaggaa
    10681 attctatata atgaaatatg catcttctta aaaaagcatc tggaagttct ttttccgcat
    10741 ttgaagttat aataactatt ggtctttgtt tagctcttac agtttctttt gtctcattta
    10801 tgtaaaactc cattttatcc aattcccaaa gcaagtcatt tggaaattct aaatccgcct
    10861 tatctatttc atctattaaa agaattactt gttgatttga gctaaaggcc tctcctagtt
    10921 taccatattt tatgtacttg ctaatatcat ctacaccttc tcctccaaat tggctatcat
    10981 aaagtctttg aacagtatca tatacataaa gcccttcttg tgcttttgta gttgatttta
    11041 tattccaaat tactaaatct ttttttaatt catttgaaat tgcttgtgct agcatagttt
    11101 tacctgttcc aggttcgccc ttaatcaaaa gtggcttttc aagagcaata gcaacattaa
    11161 ctgatgccat caattctggt gatactacat aatcactact tcctctaaag ttagatatct
    11221 ctttttttat cataaatcct actcctttta aacagtattt tctaaatgta tatagacttt
    11281 attactattt ttgatataaa aat
//
LOCUS       DAEMIK010000139        11202 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEMIK010000139 DAEMIK010000000
VERSION     DAEMIK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.141"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2091)
                     /locus_tag="KPK87_003066"
     CDS             complement(<1..2091)
                     /locus_tag="KPK87_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2539190.1"
                     /translation="MLLSIVMMVKNEENILEKTLKSLSVLRNSIESELIIVDTGSTDN
                     TIRIAKQYTEKVYFHNWNDDFSSMRNISISYAKGEWLLILDADEVLIDYSNIIKFFES
                     GLNKKFNSASVRLKNLYSFDKKLYGYCSVLRFFKNLDFKYCGKVHEQPMYKNPIFNNI
                     ADFEHYGYLFEDEEIRINKVKRNEKLLFEELKEDEKSPYTNYQIGKNLIILGKYQEAL
                     DYLEKSYKLYSKLEIVPGYLITNLAKVYLYLGKAKKCEKLCLKYINKDRNNIDIYYHI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEMSTQANSLLSDTDAVGFRDEAIITLIKIYY
                     KLEKYDLVISEYDNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYREIPNSKVERDSF
                     YKSMEDFINNIKDDEKELIYKSLCDIEGNYGLLNKIRINKHVSLDTCRKILNEEKDIV
                     YASVIKIAFEEKVDLLDIFYNMEYVWIEKYLRYILALERSFNVSIYKYIISCPNTSEI
                     EKIRVYKVISKILLESANLSDKKYKELFYIYIMYSYKYIKYIYSNLSDCELIKFVCND
                     IDKFTLEFKHVLDGKRKINNPNEKLEYLHNMRILLNEYPFYNRIIKIFISELEESITE
                     SEEFRLLKDNFIIRIENMIENGKINDAKLLIEDYSKTFADDVKILNIKAILFMCEDKF
                     EEADFALKKALSLDL"
     gene            complement(2185..3057)
                     /gene="fliC"
                     /locus_tag="KPK87_003067"
     CDS             complement(2185..3057)
                     /gene="fliC"
                     /locus_tag="KPK87_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBG2539191.1"
                     /translation="MRVNTNVSALIANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGSLEETGNILQRMRTLSLQSANEIN
                     NTEEREKIADELTQLKDEIERISSSTEFNGKKLLDGTSSTIRLQVGASYGTNVSGTSN
                     NNNEIKIQLVNTASIMASAGITTASIGSMKAGGTTGTDAAKTMVSSLDAALKSLNSSR
                     AKLGAQQNRLESTQNNLNNTLENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(3158..3493)
                     /locus_tag="KPK87_003068"
     CDS             complement(3158..3493)
                     /locus_tag="KPK87_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG2539192.1"
                     /translation="MDKLEEKINLYKDISLKIINFIEKMEYKNISFQLDERQNIINSI
                     SEVDKSEFIQLYDSMELFEIDANIRDALQEQLSEVKKELHEYKLTKQVNTMYYSSNRE
                     KVNIFNKKV"
     gene            complement(3496..5019)
                     /gene="fliD"
                     /locus_tag="KPK87_003069"
     CDS             complement(3496..5019)
                     /gene="fliD"
                     /locus_tag="KPK87_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG2539193.1"
                     /translation="MSSISPVRVTGLSGNFDMEGIIEASMIRDKEKVNKAKQDQQIVK
                     WKQEIYRDIIKESKNLYDKYLNGDSPNSITNKKAYSATRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKVTIKLNSSDPIVQQFPPNASGASSLNIGGVNIPISEQDTTSTI
                     VSKINSLCADNDIRASYSEMTGELIISRKQTGSSSDIDLKVIGNDSLAGQIASDNGIT
                     FTTDASGTKSAVVYGKNLEADVTDDQGRVTHISKEQNSFKIDNIDYNVNSKGSAKLVS
                     VTDTEEATKNMKAFVDDYNALMDKVYGLVTTKKSKDYPPLTDEQKDDMTTEEIEKWEK
                     KAKEGILRNDDELRAFVEDIQSMFFGDADTIIALRKLGISEHENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKKIVFEYSGSSASKLTKKAGMENSSSA
                     SQNVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNKYSSQMNYFSQAQGN"
     gene            complement(5047..5397)
                     /gene="fliS"
                     /locus_tag="KPK87_003070"
     CDS             complement(5047..5397)
                     /gene="fliS"
                     /locus_tag="KPK87_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG2539194.1"
                     /translation="MELDLNELNGLSKEELLLMLVDGTVKYTNISKVALLNKDYLKAH
                     NELVRVQNIFTELMVTLDQSVGQWAKDMYKVYEFIKHELVKADINKDIKIIDDILPIV
                     EQIRDTWHEVYNKL"
     gene            complement(5397..5792)
                     /gene="fliS"
                     /locus_tag="KPK87_003071"
     CDS             complement(5397..5792)
                     /gene="fliS"
                     /locus_tag="KPK87_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG2539195.1"
                     /translation="MYGENPYNSYKQNAIFMASKEQLLLMLVDGAVKYTKIARGAIID
                     KNTRKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDVQII
                     DNVLPVIEEIKDTWHEADRKSREERSRYK"
     gene            complement(5814..6026)
                     /gene="csrA"
                     /locus_tag="KPK87_003072"
     CDS             complement(5814..6026)
                     /gene="csrA"
                     /locus_tag="KPK87_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG2539196.1"
                     /translation="MLVISRKKDEAVLIGDNIEVKVVGVDGNNIKLAISAPNNISILR
                     KEIYEKVKNENIKATNKNIKILKSLK"
     gene            complement(6020..6412)
                     /locus_tag="KPK87_003073"
     CDS             complement(6020..6412)
                     /locus_tag="KPK87_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG2539197.1"
                     /translation="MMKITLKKGILGFENLKEYELLDIENEDILKEFNSTEEDCIGFI
                     VVSPFEIIKEYEIVLNQETIEKLEVKSPNDIMLLNIITVGQTLEESTVNMKAPIVINV
                     RNNCGMQIILQDEEYSIWHPLLRGDGGC"
     gene            complement(6428..7360)
                     /gene="flgL"
                     /locus_tag="KPK87_003074"
     CDS             complement(6428..7360)
                     /gene="flgL"
                     /locus_tag="KPK87_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG2539198.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQINTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYIENCKDTADWLETTDTSLDQLGNLLADIKKGLVSSGNGSY
                     SDDEIKTISNSTNEKMKEIANALNATHEGKYIFSGSNTGTPPVECVENADGSVSLKFN
                     TSLNLNKLNDSLSVDVAQGISVDYNVKLSSLGFDPTKTDPFEKLNNISKKLANPSDAN
                     IKELTTTCLGDMENLIDNTVNVRSIYGTKANTVDAMKEKNDEGLIQLKDVLSQNDEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(7379..8689)
                     /gene="flgK"
                     /locus_tag="KPK87_003075"
     CDS             complement(7379..8689)
                     /gene="flgK"
                     /locus_tag="KPK87_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG2539199.1"
                     /translation="MAGLFSILNTANSGMNAHQKSIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIPNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNSSAGITVRDFFITSNVAGEDIIKVNPAIKSNSNELKLTTEEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNVDNSR
                     MSVSGVSLDEEMTELMKVQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(8707..9123)
                     /locus_tag="KPK87_003076"
     CDS             complement(8707..9123)
                     /locus_tag="KPK87_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG2539200.1"
                     /translation="MITDLKVIIYEEKKIISNMMELLKEQFDYIIEKDLENLNRLNPK
                     LEDISRELASIEIKRRQLLGDDVSISEVVENSNDEYLKEIYIDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            complement(9128..9403)
                     /gene="flgM"
                     /locus_tag="KPK87_003077"
     CDS             complement(9128..9403)
                     /gene="flgM"
                     /locus_tag="KPK87_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG2539201.1"
                     /translation="MNIKSVSSNIVVNNYLKNKSDVKKVQQVQKVDKTDKIELSKFAK
                     EMSSVSNMSDEAARNKKIEEIKSALENGTYKIKPEEIAKKMIEEMRG"
     gene            complement(9554..9868)
                     /locus_tag="KPK87_003078"
     CDS             complement(9554..9868)
                     /locus_tag="KPK87_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2539202.1"
                     /translation="MNSKKEDIYELNFGQLIEEKTGIIDENQNVFNAKVNVAVSIGNI
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNETLSVRLSKIEDSD
                     VN"
     gene            complement(9892..10320)
                     /locus_tag="KPK87_003079"
     CDS             complement(9892..10320)
                     /locus_tag="KPK87_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG2539203.1"
                     /translation="MGINIDKLNISPSRSIEVKKTKENNKFSDSLNFVKQSSSLEDIK
                     RNLDGVKKAGERLVLTQNYGDISRYKNAVKEYLKSVVDNMYSLDKSSSFWEHQYYKNV
                     KIIDNKLEDMTSKLLSEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(10329..10997)
                     /locus_tag="KPK87_003080"
     CDS             complement(10329..10997)
                     /locus_tag="KPK87_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBG2539204.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCGYNNSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSEKLFNELDSVANNTVGKFIEVDTKDKSVKLRIENAVEQA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTNPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMRSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
BASE COUNT         3338 a         1971 c         1113 g         4780 t
ORIGIN      
        1 taagtctagt gataatgcct tttttaatgc aaaatcagct tcttcaaatt tatcttcaca
       61 cataaacaaa attgccttta tatttaaaat ctttacatca tctgcaaatg tttttgagta
      121 gtcttcaatc aataactttg catcatttat ttttccattc tcaatcatat tttctatacg
      181 aataataaag ttatctttta ataatctaaa ttcttcactt tctgttatac tttcttctaa
      241 ttcacttatg aagattttta ttattctatt gtaaaaaggg tattcattta ataatattct
      301 catattatgt agatattcta atttttcatt aggattattt atcttccttt taccatcaag
      361 tacatgctta aattctaaag tgaatttatc tatgtcatta caaacaaact ttattaattc
      421 acaatcactc aaattgctat atatgtattt tatgtattta taagagtaca ttatataaat
      481 ataaaaaagt tctttgtatt ttttatcact taaattagca ctttctaaaa gaattttaga
      541 tataactttg tacactctta tcttttctat ttctgatgta ttaggacaac taattatata
      601 tttatatatt gatacattaa aacttctttc taaagctaaa atatacctta agtatttctc
      661 tatccaaaca tattccatat tgtaaaaaat gtctaataag tcaacttttt cctcaaaagc
      721 tatttttata acacttgcat aaactatgtc tttttcttca tttagtattt ttctacatgt
      781 atctaatgat acatgtttgt ttattcttat tttattcaaa agaccataat taccttcaat
      841 atcacaaagg cttttatata tgagctcttt ctcatcatct tttatattat ttataaagtc
      901 ttccatactt ttataaaaac tatctctctc aaccttcgaa tttggaatct ctctataata
      961 gttttttatg tcttcaaatc tatttaattt atataaagac ataaataagc taaagtacac
     1021 atttttcttt ttttccacat cttcaatatt atcatactct gaaattacta aatcatattt
     1081 ctcaagctta taataaattt ttatcaatgt aattatagct tcgtctctaa atcctacagc
     1141 atcagtatct gaaagcaaac tatttgcttg tgtactcatt tcataattat ctagcaaata
     1201 aatatatctt ttatatgaat ctatactatt ttcatacttc cctaaatcaa cttgtgcttg
     1261 tgcaatatga taatatatat caatattatt tctgtcctta ttaatatatt ttaagcataa
     1321 tttttcacac ttcttagcct ttcctaagta taaatatact tttgcaaggt ttgttatcaa
     1381 gtatccaggt actatttcta attttgagta taatttataa gacttttcta gataatctaa
     1441 agcttcttga tattttccta atataataag atttttccct atttgataat ttgtatatgg
     1501 agatttctcg tcttctttta attcctcaaa caacagtttt tcatttcttt ttactttatt
     1561 tattcttatc tcttcatctt caaacaagta tccataatgt tcaaaatcag caatattatt
     1621 aaatatagga tttttataca ttggttgttc atgaactttc ccacagtatt taaaatcaag
     1681 gtttttgaaa aatctcaaca ctgaacaata tccatataat tttttatcaa atgaataaag
     1741 attttttaat cgtactgagg cagagttaaa ttttttatta agaccacttt caaaaaactt
     1801 tataatattt gaataatcaa ttaatacttc atctgcatct aatataagta accattcgcc
     1861 cttagcgtag gatatagata tatttctcat actagaaaaa tcatcattcc aattatgaaa
     1921 atataccttt tctgtatatt gttttgctat tcttatagta ttatctgtag aacctgtatc
     1981 aactattata agttcactct ctattgaatt tctcaatact gataacgatt ttaaagtttt
     2041 ttcaagtata ttttcttcgt ttttgaccat cataactata ctaagtaaca tatatatttt
     2101 cctttcaaaa ctatatttct aaaaaaagaa aagaataaag caaagcctta tcctttcttt
     2161 tttattcaat atactaaatt tattctatcc taataattgt aaaactcctt gtggttgttg
     2221 attagcttga gcaagcattg attgtgatgc ttgaactaat atattcattt tagataagtt
     2281 aaccatttct gaagcaacat ctgtatctct tattcttgat tctgctgctg taacattctc
     2341 taaagtatta tttaaattgt tttgagttga ttctagtctg ttttgttgtg ctcctaattt
     2401 tgctcttgat gagtttaagc tcttaagagc tgcatctaag ctagatacca tagttttagc
     2461 tgcatctgtt cctgtagttc cacctgcctt catacttcct attgaagcag ttgttatacc
     2521 agctgaagcc attatacttg cagtatttac taattgaatt tttatctcat tgttattatt
     2581 acttgtacct gaaacattag ttccataact tgctccaact tgtaatctta ttgttgaaga
     2641 agttccatca agtagcttct taccgttaaa ctctgttgaa cttgatattc tttcaatctc
     2701 atcttttaat tgtgttaact catcagctat cttttctctt tcttcagtat tattaatttc
     2761 atttgcagat tgtaatgata aagttctcat tctttgtagt atatttccag tttcttctaa
     2821 cgaaccttct gctgtttgta caacagatat accatcttga acgtttcttc ctgcttggtc
     2881 taaacctttt atttgagctc tcattttctc agatattgca agtccagcag catcatcagc
     2941 agctctcttg attctaaccc cagaagataa cttctccata gacttacttt gtccatttac
     3001 gtttcttccc atttgattgt ttgctatcaa agcacttaca tttgtattaa ctctcattat
     3061 tttaccctcc ttagtatagt tgacatcctt gtcatttcta cttttaactt ttgtattaac
     3121 tatccgttta tattatcgga caagttaatc tataacttta tactttttta ttaaaaatat
     3181 ttactttttc tctatttgaa ctataataca ttgtgtttac ctgtttagta agcttgtact
     3241 catgtaattc tttttttact tctgataatt gttcttgcaa agcatctctt atattagcat
     3301 caatttcaaa tagctccata ctatcataaa gttgtataaa ttcactttta tcaacttcac
     3361 ttatactatt tataatgttt tgtctctcat ctaattgaaa acttatattt ttatactcca
     3421 ttttttcaat aaaatttata atttttaaag atatatcttt atataaattt attttttctt
     3481 ctaacttgtc cataattaat taccttgtgc ttgtgagaaa tagttcattt gtgaagaata
     3541 tttattcaac aaagactcta aagctgaata tttagcataa agtcttttct ctttatcatt
     3601 cattttttca actaacctac ttatatttct ttcttgctct gcaatttgtt ttgaatatac
     3661 attttgacta gcagacgaag aattttccat acctgctttt ttagtaagtt tagaagctga
     3721 acttccagaa tattcaaata caattttttt taatttttca aacattccct tatcatcata
     3781 atttgaagaa tatccagcta aagttttata taccttgtca ctatcatcta taagagcctt
     3841 cgaaaaagta tctgcattaa atgatatttg tccttttttg ttataattct catgctcact
     3901 aatacccagt tttcttaaag caataatagt atctgcatct ccaaaaaaca tagactgaat
     3961 atcttcaaca aaagctctta attcatcatc atttctaagt attccttcct ttgccttctt
     4021 ttcccacttt tctatttctt cagttgtcat atcatctttt tgttcatctg taagaggtgg
     4081 ataatctttt gattttttag tagtaactaa tccataaacc ttatccatca atgcattata
     4141 atcatctaca aatgctttca tatttttagt agcttcttca gtgtcagtaa cagaaaccag
     4201 tttagcactt ccttttgaat ttacattata atcaatgtta tctattttaa atgaattctg
     4261 ttctttactt atgtgtgtta ctcttccttg gtcatcagtt acatcagctt ctaaattttt
     4321 tccatatact actgcagatt tagttccact agcatcagtt gtaaatgtaa taccattatc
     4381 actggcaatt tgtccagcta aactatcatt tccaattact tttaaatcaa tatctgatga
     4441 tgaaccagtc tgttttcttg agataatcaa ttcacctgtc atctcactat aagaagctct
     4501 tatatcatta tctgcacaaa gggagtttat cttacttaca atagtacttg tagtatcttg
     4561 ttcagatatt ggtatattta caccacctat atttaaagaa cttgctccac tagcatttgg
     4621 aggaaactgt tgaacaatag ggtcacttga atttaactta atagttactt ttgctggttc
     4681 agccatttga gaaactgcaa attgataatt tattttttct gcaccagctg agccttttgc
     4741 tactataata ctttcatcag aactagttat tcttgtagca gaatatgctt ttttatttgt
     4801 tatactatta ggagagtctc catttaaata cttgtcataa agattctttg actcttttat
     4861 aatatccctg tatatttctt gtttccattt aactatttgt tgatcttgtt tcgctttatt
     4921 aactttttcc ttatctctta tcatactagc ttctattata ccttccatat caaaatttcc
     4981 tgaaaggcct gtaactctta ctggacttat acttgacata tgtttatccc cccatcatga
     5041 ttatagttat agtttattat acacttcatg ccatgtatct cttatttgtt ctactattgg
     5101 caatatatca tcaattattt taatatcttt atttatatca gcctttacta attcatgttt
     5161 tatgaattca taaactttat acatatcctt tgcccattgt cctacacttt ggtctaatgt
     5221 gaccattaat tctgtaaata tattttgaac tctaacaagt tcattgtgtg cttttaaata
     5281 gtctttattt aaaagtgcaa ctttagatat attagtatac ttgactgtac catctacaag
     5341 cattaatagt aattcttctt ttgataaccc atttagttca tttaaatcta actccatcat
     5401 ttatacctac ttctttcttc tctacttttt ctatcagcct catgccatgt atctttaatt
     5461 tcttctatta caggtaatac attatctatt atttgtacat cttttctgat atttgccctt
     5521 gaaagttcat atcttacaaa atcataaact ctatacatat ctttcgccca ttgacctgca
     5581 ttttggtcta atgtaaccat tagttctgta aatatatctt gaactcttat aagctctcta
     5641 tgtgcttttc gtgtattttt atctataata gctcctctag ctatttttgt atattttact
     5701 gcaccatcaa caagcataag caataattgt tcttttgacg ccataaaaat agcattttgc
     5761 ttataagaat tataaggatt ttctccgtac atatattact tcctccaagt gttttatttt
     5821 aatgatttta agatttttat atttttattt gtggctttta tattttcatt ttttactttt
     5881 tcatatattt cttttcttaa gatacttata ttattaggag cagatatagc taattttatg
     5941 ttatttccat ccactcctac aacttttact tctatgttat ctcctataag tactgcttca
     6001 tctttttttc tcgaaattac tagcatccac catcacctct caataatgga tgccatattg
     6061 aatattcctc atcctgtaaa attatttgca ttccgcaatt atttcttaca tttattacta
     6121 taggagcttt catgttcaca gttgattctt caagtgtttg accaacagtg attatattaa
     6181 gcaacataat atcatttgga gattttactt caagtttttc tattgtttct tgatttagga
     6241 ctatttcata ttccttaata atttcaaatg gtgatacaac aataaatcct atacaatctt
     6301 cttcagtaga attaaattct tttaaaatat cttcattttc tatatctaaa agctcatact
     6361 cttttaagtt ttcaaatcct agtattcctt tttttaatgt aatcttcatc atatctgcgt
     6421 tattttacta tatataatct aatatagata cattaaataa ctttcctcct gtttgtagtg
     6481 aagcttgata agttaattct gcagccttta attgaacaag cttttcccca taatctattt
     6541 catcattttg cgataaaaca tcctttaact gtatcaaacc ttcatcattt ttttctttca
     6601 tggcatctac tgtgtttgcc tttgtaccat atatagacct aacatttact gtattatcta
     6661 ttagattctc catgtcacct aaacaagttg ttgtcaactc ttttatattt gcatcacttg
     6721 gatttgccag tttcttcgat atattattta acttttcaaa tggatcagtt tttgttggat
     6781 caaacccaag actactcaac ttaacattat aatctacact gattccttga gctacatcta
     6841 cacttaaaga atcatttaat ttattaagat ttaagctagt attaaacttt aaacttactg
     6901 agccatcagc attctctaca cactcaacag gtggtgtgcc tgtatttgaa cctgaaaata
     6961 tgtacttacc ttcatgtgtt gcatttaaag catttgctat ttctttcatt ttttcattgg
     7021 ttgaattact aatagttttt atttcgtcat ctgaatatga accattccca cttgatacca
     7081 aacccttctt tatatctgct aataaattac caagttggtc taatgaagta tcagttgttt
     7141 ctaaccaatc tgctgtatct ttacaatttt caatataagt ttccatagtt tttatttcac
     7201 tttttgaatt caatatcttt atcgccttat atggattatc tgatagttta tttatttctt
     7261 tcgttgtgtt gatttgtcta ttgaccttgt ctaatctttg caaattatct tgcaatgaat
     7321 ttagataact actggacatc atacctgtac taactctcat tataaattcc tccttttatt
     7381 aaattatccc atttattaca acatccaata gttgtactgc tgttgacata acttttgcac
     7441 tagcttgata ggaacgttgt actttcatga gttctgtcat ttcttcatct aaagatacac
     7501 ctgaaacact cattcgtgaa ttatcaacat tatttataat tttacgttga ttcacttcat
     7561 cttgattaac tttttgagaa tctaaaccta ccgattctgc aaaagccttg tagtggtcac
     7621 ttattgtgct tacttttcca ccagctaccc caatatctat tttttcatcc tttaacttag
     7681 caatctttga agcttcttca gtagtaagct tcaattcatt tgaattactt tttattgctg
     7741 gatttacttt tataatatct tcaccagcta catttgaggt tataaaaaaa tctcttacag
     7801 ttattccagc agatgaatta taaacatcat ttatctgttt tgctaatcca tcagatacat
     7861 cttttaagtc gcttttatat gtatcaattt taccgtgcat ttcaaacaag ccttgtaaag
     7921 ttcctgatat acctgttttc caagttccat ctgcattttt caactcatta agttccaatt
     7981 taccatctga aagcatattt ttaacatcac tattttcaag gtctaactta aaactcaagt
     8041 tatccaatat tctatctctt tcatcaagca aatcatttgg tgtagagcca gaaccagaga
     8101 taatatcaat agatttattt aagtcttcaa gactttttaa catgtcattt atttgtttta
     8161 tttcagtatc tatattagaa tctatatctc cttttaactt gtcaagcttc ttataacttt
     8221 cgcttattaa agtcgctaaa ttagatgcat tttctaccac tagacttttt ttactatcac
     8281 tatttatatc ctttgataat gactcccatc ctttaaaaaa atcatctata cctgcagata
     8341 ttccatagtc tgatggttca tttggtatag tttctatagt attataataa tcatattttg
     8401 aaactgtatt cccatactta tgagcttctg ccctaaattg gtaatcataa aaactatttc
     8461 tagctcttgt tacatctgtt acatggactc cagtacctaa ttgtcctctt ccaattgaat
     8521 tgctcaaact aggcatatac attggactat ttgtagcaaa ttcggtcctt tgcctagaat
     8581 aaccatctgt atctaggtta cttatattat gagaaattgt ctgtattgat ttttggtgcg
     8641 cattcattcc tgaattagct gtatttaaaa tggaaaataa tcctgccata tttctcctcc
     8701 caatatctat tttctaatgt ttccataaga attataagtt gtagtttgtt tatcaactgg
     8761 ttttatgaaa ttaatcattt ttttcgtgaa aataagttct ttctttatta gagaatcatt
     8821 tgattcttgt tgatttttag ctaaagcaag aatatgcttt aagtctatat atatttcttt
     8881 taaatattca tcattactgt tttcaacaac ctcacttatt gaaacatcat cccctaaaag
     8941 ttgtcttctt tttatttcta tagatgcaag ttctctacta atatcttcta gtttaggatt
     9001 caacctattt aaattttcta agtctttttc aattatataa tcaaattgtt cttttagtaa
     9061 ttccatcata ttactaatta tctttttttc ttcatatatt atgactttta agtctgtaat
     9121 cattacttta tcctcgcatc tcctctatca tttttttagc aatttcttct ggtttaattt
     9181 tgtaagttcc attttctaat gcacttttta tttcttctat ttttttattt ctagccgctt
     9241 catcgctcat atttgataca cttgacattt cctttgcaaa tttagatagt tctattttgt
     9301 ctgttttatc aactttttga acttgttgta ccttttttac atccgatttg ttctttagat
     9361 aattatttac tacaatattt gaacttacac ttttaatatt cataacctca cctccaaaat
     9421 tacatcctta tattttaatt atcgctcaaa gaagaaaaat atttatacga ttcaaataga
     9481 ttttaaattt tttataaaaa taccccttga ttaaaccaat atagaataat ctaaggggat
     9541 attttttaaa gtattaattt acgtctgaat cttctatttt agataatctt acagataatg
     9601 tttcatttgt aataatactt tctccataag caaacaatct gtcatttgca tatatgtcta
     9661 atttttcatc tacattctta tttagtttta ttatagaacc ttcttttaaa tctaatatat
     9721 tttttattgt ttctgttatg tttcctatag aaacagccac atttacttta gcattaaaaa
     9781 cattttgatt ttcatctata atcccagtct tttcttctat taattgacca aaattcaatt
     9841 catatatatc ttcttttttg ctattcataa aactattctc cttatgtaat cttatctata
     9901 catatctatc aataagcctt gtatcatatc aacagtagaa acaatatcta tattttcttt
     9961 ttcttcacta agtagtttac tagtcatatc ctcaagttta ttatcaataa tctttacatt
    10021 tttataatat tgatgctccc agaaactact acttttatca agtgaataca tattatctac
    10081 aacagatttt aggtattctt ttactgcatt tttatatcta ctaatgtcac catagttttg
    10141 tgtaagtaca agcctctccc cagccttttt tacaccatct aaattccttt ttatatcttc
    10201 tagtgaagaa gattgtttta caaagtttaa gctgtcagaa aacttattat tttcctttgt
    10261 ctttttaact tctatacttc tacttggact tatatttagc ttgtctatat ttatacccat
    10321 agtaaatcct accctctata atatttcata actttaggta tataattttg tgtctccttt
    10381 ggcattttat acaagtcaga aattgattct acgcctctac ttctcattct accaggtcca
    10441 ccattatatg ccatcaatcc catttctatg ctacctccaa acatatctat atattcctta
    10501 atatgtctag taccagcatc tatattttgt tctatgttaa aagggtttgt tactcccaaa
    10561 tctctgcaat tttctggcat aagttgcata agacccttgg caccagcaga agatacagta
    10621 tttgggttaa aatctgattc aaccttaatt attgccttta taagatttgc atctacatta
    10681 tatttctttg atgcttgctc tacagcattt tcaattctca actttacact tttatctttt
    10741 gtatcaactt caataaactt accaactgta ttatttgcta cactatcaag ttcattaaac
    10801 agtttttcag aattagatga taaattatat ccattagagg tttgttgatt attttgtgat
    10861 aatgctttca acaatgtcat cataatcata tcaaatccag atgaattatt atatccacag
    10921 ttgcattgat ttgtggtatt actaccagat aattgggtca atgccagtat atttattagt
    10981 gaacttattt cgttcaaaat aaaactcccc tttctaaatt ttataaactt taaagttatc
    11041 cttatataat tcaagatact tgagttccta taaaatcttc ttgttataga ctactttgtt
    11101 aaattaattt cttttatatt cattaaaact ttagatttag tttcactaca ttatgattat
    11161 cggataaata aaaaaaatat ttatactaaa attatttact aa
//
LOCUS       DAEMIK010000140        11116 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEMIK010000140 DAEMIK010000000
VERSION     DAEMIK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.142"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(412..588)
                     /locus_tag="KPK87_003081"
     CDS             complement(412..588)
                     /locus_tag="KPK87_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539205.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(692..1300)
                     /locus_tag="KPK87_003082"
     CDS             complement(692..1300)
                     /locus_tag="KPK87_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2539206.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            complement(1399..2223)
                     /locus_tag="KPK87_003083"
     CDS             complement(1399..2223)
                     /locus_tag="KPK87_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2539207.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(2401..3294)
                     /locus_tag="KPK87_003084"
     CDS             complement(2401..3294)
                     /locus_tag="KPK87_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2539208.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(3551..3988)
                     /locus_tag="KPK87_003085"
     CDS             complement(3551..3988)
                     /locus_tag="KPK87_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG2539209.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(4245..4394)
                     /locus_tag="KPK87_003086"
     CDS             complement(4245..4394)
                     /locus_tag="KPK87_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG2539210.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(4407..4637)
                     /locus_tag="KPK87_003087"
     CDS             complement(4407..4637)
                     /locus_tag="KPK87_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG2539211.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVKTNTTPYDLVIE"
     gene            complement(5083..6276)
                     /locus_tag="KPK87_003088"
     CDS             complement(5083..6276)
                     /locus_tag="KPK87_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2539212.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLDKQLINVDHQLLKIADVGYYVETPKTKSGNRIIPMSE
                     KVLEAFQRVLNKRKYTQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
     gene            complement(6356..6559)
                     /locus_tag="KPK87_003089"
     CDS             complement(6356..6559)
                     /locus_tag="KPK87_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HBG2539213.1"
                     /translation="MNNNDVPIWEKYTLTIEEAAKYFRIGESKLRKLAEENPVPDWVM
                     MNGNRIQIKRKQFEKMIDTVDAI"
     gene            complement(6981..7238)
                     /locus_tag="KPK87_003090"
     CDS             complement(6981..7238)
                     /locus_tag="KPK87_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539214.1"
                     /translation="MNGTNGNQNDYPENALVPYPVILAASKGDPDAMKMVLQHFSGYI
                     ASLSMRKLYDERGNVYYGVDEDIRERLQARLMRAILTFRAE"
     gene            complement(7219..7659)
                     /locus_tag="KPK87_003091"
     CDS             complement(7219..7659)
                     /locus_tag="KPK87_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBG2539215.1"
                     /translation="MEPNRREFQKQCAFQSFCKRVLHNEACNAHDEIRRRRKREVSFC
                     DLALHEERQLYTTDKYFQAETAEPTYQMAGKEITPKLLLEAIRTLPEEKRTAILLYYF
                     EGMTDVEIGKMFNTSRSTIQYRRTSSFELLKKYLEEHADEWNEW"
     gene            complement(8132..8905)
                     /locus_tag="KPK87_003092"
     CDS             complement(8132..8905)
                     /locus_tag="KPK87_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539216.1"
                     /translation="MRNIIKTEFLKLKRYSVIKAGIIMTTLSPLLSLFYSTANGGQTW
                     TFDYFMQQVMISNCTLFFPIIIALIAGYIITREYTDDTMKNILTIPIPYKQLLSGKLL
                     ILLLLTISFSLIGCVIALVINIIVGFPGVHFGNLLNMFIRVTGANIGIYISVLPIILI
                     FCCSANNFLGGVALAFVYGYFGTFEGTLLNYYPIKASMILVDPTCGTEYGYTYHIFPA
                     FITIVLTFLISMIILANKKKEPGTLTVGKKKKAVRKKGW"
     gene            complement(8911..9720)
                     /locus_tag="KPK87_003093"
     CDS             complement(8911..9720)
                     /locus_tag="KPK87_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539217.1"
                     /translation="MLKLIKCEFWKLKRKRLLYALMSLSLFFPLILAYMAKAGTGADT
                     TEHYLQTRFDYVYTMMLGYGLVFLLPCLIGIIAAILFFIERDCDTSKNLRTIPVTNTQ
                     LIMAKISMLFIFSVAFCLISTLSVALFCKLFHVGMVYGMTYKIFMSLIFGVLIVAASL
                     PIVFLIIYFNKSFLLSILLAFFYSIFNWGILGTIGTSISAAKIAFLNYFPVICVMNWT
                     SGLMMDNLQKDNLLPEAYAIVPTTCHTIFIMAITVILSLWLIIRFYKKWTR"
     gene            complement(9713..10639)
                     /locus_tag="KPK87_003094"
     CDS             complement(9713..10639)
                     /locus_tag="KPK87_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539218.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGIPNRHSIKNTLDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGTLLEEES
                     LAELEQKSSKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            complement(10776..>11116)
                     /locus_tag="KPK87_003095"
     CDS             complement(10776..>11116)
                     /locus_tag="KPK87_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2539219.1"
                     /translation="VKLDTDGYMRILNNLIQNVISHSHADKIEIILSKQEKNMQIRLA
                     DNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQLVEKMNGTITANSIQGTGT
                     EFTLLFPLAN"
BASE COUNT         3517 a         1998 c         1754 g         3847 t
ORIGIN      
        1 taataaagaa ctgtaataaa taagacttta caatgtaata gaaatgctgt aataataaag
       61 gtctgtagta taataaagaa ctgtaataaa taagacttta caatataata gaaatgttgc
      121 aataacaaaa caccataata aataagagtt tgcaatataa tagaaacact gcaataaata
      181 agagattgta atataataaa agtctgtaat ataacaaaat cctataataa ataagatttt
      241 acaatataat aaaaatccta taataaatag gactctgtaa tataataaaa tgctgtaaca
      301 acaaaacact ataataaata agaccctgta atataataaa aatactataa tatagtaaaa
      361 atcatgcaat aataaaaatc tataataata aacctcagca taacaaagac attaatttct
      421 tactaaaaag gccaccacaa ttaacagtga aaaagcaatt gaaagtattg aaaaagtaaa
      481 gtgagaatta gagtcattat tacgattatt aagttttatc cataaaccac atataattgt
      541 acacaacaat aaaattgctg atattactcc tataaatgta aaaaacatta aaattcctcc
      601 cccttattta atgcaagtat ttttagtcaa tgagaatatt ttcaaacgtt accataaaat
      661 aaaaaaatgt acataaaaaa ttaatatagt attattttgt aatatctacc aataataaat
      721 ctaattgaaa atctattaaa ccatttctct gttcaacatt aagaatatca ataccactat
      781 acttgaaaaa agcattagaa cgaagaaaaa gcaactgaat aaatgaagtt atttgatacg
      841 caatagttct gtattcagct tcagtttttt tgttattctg attttcatag tgagccttaa
      901 ttaaaggtag tatctcaaat ggataaacaa tttcttcctg tatcaatata tagggtacag
      961 aaatctttat aaattgacta tgttcaccta taaaatcaaa aaatgcaatt agtatttccc
     1021 ttaattgctg tttaggtgct aaatttttag tatcatgtgt agccttatat tcatccatat
     1081 gacgctttaa aaatttttta atagaaatat taattaactc ctctttagat ttaaaataat
     1141 aattaatcat agctaaattg acatttgctt tagctacaat ttctctagcc gtaatgcttg
     1201 aaacatctct atgtgaagat agtaactgga ttgtggcatt aattaattgt tcctttgttg
     1261 aatctgcttt cataattttt ccccttaaaa gtttctccat aaaaaaatta aacatgttta
     1321 atacacgttt agatactatc atgaaaattt tgggatgtca atgggtgatt agtacttaag
     1381 tatttaagta ctaatcgatt agacttttat aagggggatt tgaatttctg taagataatt
     1441 ttcaggattt atttcattat gttcaccttt atgaccgatt tgtctattta tacctgatat
     1501 ttgataatca ctatttttat ctaaccaata acagaacgat tcatatgcac cagctatatt
     1561 ttcatatgga ccataaacca tagtacatgc catcatatca atttcttctg tctcacgata
     1621 tataaatcca gatttgttct ttccaatctt ctttacaacc attccaactt caatatctac
     1681 accattatct ttgacttctt catcatggta aaaagctatg ttattgttgg attgacgtga
     1741 tacttctata tgttcttctt tgataaattt agatagcttt tcccagagta taccttcaga
     1801 ttggtagtct ggaattatct ctctaagtga tatgatttta taacttggaa tctttttaaa
     1861 gacaacattg taatgaattt catctttatc acagtttata gcttctataa atttatcaat
     1921 tttatttatt ctttgttgtt cctgctttat ttccttttgt atttcattct tttttctatt
     1981 caattcctta attacaaatt catcattcca attatgtacg atatttgcta tttctgcaac
     2041 gctaaatttt gaatctctca ataatacaat tctttgtaat attgaaatct gttcaactga
     2101 gtataatcta tgacctgtat atttgtctgt ttttgcaggt tttaaaatac caagttcatc
     2161 ataataacgc aacatacgaa tagaaatttg tgttagtttt gaaacctctc ctattttaaa
     2221 catgaaaata tgacctcctt ttcacttata atcagtatat attttgtttt tatatacttc
     2281 aagcgtttgt ggaaatacaa ttattttcca gatgttgcaa taaattatag agagtaaatt
     2341 atatttaagt agtaatcgta tcaaatcata gattatctta atttataatt catattaaaa
     2401 ttaccagtct aactcaaatt caaaatcatc cgactttatc tgttcaattt cagaatttac
     2461 cttctgttta aatttattag aagaaatcat atatacaaga aatattaaac caaatattat
     2521 ttgtacatat tgagctatta cacataattt aatagggtag tgtagaagta attgaatgtt
     2581 gaaaaaatag tacatcatat gaactaaccc agctgataaa atgcttctac ttgtattgat
     2641 atatacaaaa ttaattataa agtttaaaag tacaatgttt ggtataaata aaaaagcatc
     2701 aaatagggag tattgtgcca aattatatgg tatttgatta ggtgtaaaat acgaccctag
     2761 ataccagatt ccacaaacaa gccctaaaat aattgaagag cctataaatc caaatctaac
     2821 taataatttg tcaatggcat atcctctcca tccagcttct tgactaagta tactcaatat
     2881 aaaacttgct aatagcatat aagctatttt agaaggtcta aacactgcta tgtgtatcca
     2941 atctatatca gaagttttta cattaaagta atttccgatt attaacgaag taataaaagt
     3001 taaagcaaaa aataatattg ttaatagtat ccattttatt ctaatttctt taaagcttag
     3061 gtatctataa ataaaatctt gttttgcact ttgtggatag attgtagaaa taaaaaatag
     3121 acctacaata gctggtgatg tatatgcaag ataaaaaatg atttctccaa gtattgtatt
     3181 tgtaattccg agcaacgcag gtgtaagact gaatatccag ttcaatacta gcgtaaatgt
     3241 aaagaaatag attaagtgtt tgtttgtatt aaagggggtg ttatatagtt gcattttttc
     3301 actatccttc cgttttataa tgaagtttta atttttatta attgtttatt tatttttata
     3361 tgtattaagt tatcgcttga tgttagttat tgcctataat aatatcctat tttttatatt
     3421 ttgattaatt atttttacac taaatataat taattagtat ttaatattga atatattata
     3481 ttattattaa ttattaaata ggtcaataaa tttgcaatta gtgatattaa taaattactt
     3541 tagattttta ttaaatgttt agcttttctt taatattttt tatataacga cgatttataa
     3601 taactttttc gttgtttttt aaagtagctt gcatgcgatt atgtgatatt acttttatac
     3661 tttttatgta gtccatattt aaaatcatgg atttagatat tcgtgagaag gatgtattag
     3721 ctaataattt tgataattta tatattttta aatcaatttc aaatacatca tcttcataat
     3781 aagcaaatgt tttatttcca actgtctcaa agtataaaat atcacagaca ttgagtttat
     3841 aaatctctcc tgatttttta ccttttatat caagtaacgg tgtactcaat aatgaaatta
     3901 ttttttcaat gttattatct agagcagaac attttatggt tatttcaata tcatcacaat
     3961 taataccttg ttcgacatgt attttcatta atcatcacct cattttatta ctgtcattag
     4021 aatagcatta tatcttattg agtccaacta gatgcgttct aaatttcaaa ttaagcatag
     4081 tgaaattcaa aaataaaaca gtgaaattca aatttgaggt ggtaaaattc aaaaaaatac
     4141 tatggatatt tggataaatt atccaaatat cctagtattt cacatagaat atgtctaatt
     4201 attttacaaa tagacctatt ttatgactta catatattgt caaattactt cttaaatatc
     4261 aaaatatttt tggataatgt atcaatactg tttgataaag tatttaattt accctcaatt
     4321 ctaaccaata agtacatgga tagagctata ggaaaaccaa cattggttat taacagttgt
     4381 aaactatcat ccataaaatt agccccctat tctataacta agtcataagg agtggtattt
     4441 gttttcacaa ccttggcttc aacaacagaa gctaggtcaa atcctccaac cgaaaaaata
     4501 tttttagata ctatcaaatc catgacagat tttatatttt cttcagttaa atcatccttt
     4561 ggattttcta tagaaaaaga aacttgttta tcctttacat ttttaaagct catgactaat
     4621 tttttcttaa tttccatata attacctccg atataataat ttatatttta attgtttgct
     4681 tattaatatt ggtattttaa ttgtcaatat tagtgttttg cttgttagta ttagtatttc
     4741 aattgtcaat attagtgttt tgcttgttag tatcagtgtt tcaattgtca atattagtat
     4801 tttaattgtt gactctagtt attgctgata tagaaaatat ttaatttatt tcaattaatt
     4861 agattaaagt agggtagcat ctaaaatagc taacatgttt aagttgaaat cacaaagtaa
     4921 tttttgacta tcttttcaaa agttcttata tgccggtata gcctattgtt acaggctttt
     4981 ccggcatttt tcgtcatgga agatattctc aaatcttctt atatccccgt atgttttggt
     5041 gttcaaaagt agtatgtgga gtagtaaatt ctccatttac tactatgcaa tcagccgctt
     5101 catttctgtt attgacgttt cggaagttgt gtgtgcgtaa tagttcagcg tcatattgat
     5161 gttggaatgt cccatgatgt actgcaatgc tttggggttc attcccgcat ttgctaacgt
     5221 ggtgcagaat gtgtggcgta aggtgtgcgg tgtcattacc tttggcaatg ctaccttttg
     5281 cgttttgttg tatttcctta caagcccccg gaacatactt tcatagttga ctgccacttt
     5341 tggcaaaccg ttccggttaa ggaacagaaa ctttgtgtag ccctctaaaa taacaggctg
     5401 tgtatatttc cgtttgttca gcacccgctg aaatgcttcc aacacttttt cactcatagg
     5461 aataatccgg ttgccgcttt tagtcttagg cgtttctaca tagtagccca cgtctgcaat
     5521 cttcaaaagt tggtggtcta cattgataag ctgtttatct aagtcaatgt cagcttccgt
     5581 caatccgcag agttccgaaa tgcgaagccc tgtccctaac agtatgataa tctcgtcgta
     5641 gtatttctga tagactttat catgctgcac aaaggacagg aaagccgctt cctgcgtagg
     5701 ggatagaggt tcctttggtt ccgtatcatc ttcaaggact gtgttcaact ggaagtcaaa
     5761 cggattttta cgaatacaat cgtcctgtat ggctgtgtaa aaggcagcct ttaaggaacg
     5821 cttgtggttg ttgatagtct tgaagccata acctttttct ttcatgcgta atgcccattc
     5881 ctttgcgtcg gagagcttta cattttcaat ccggcaggct ccaagtttat cctcctgcaa
     5941 aatccgcatg agctgttttc gcccctgctt tgtaccatgc ctcacatttg cccggtggcg
     6001 tatctgcttt gcgtaaagct ggcagactgt cattttcttt ccgatatggt cgataccgtc
     6061 gtcaaggtct ttttggattt ctttctcttt ttccctcaac gctatatcgt cgcgctttcc
     6121 tgccggggtt ttgtccgtag gtactaactt ccaagaatac acaaactgcg gttttccaaa
     6181 ggtatctata tatttgtagg catatcttcc gtcttttctc tggctctctc cggtgcggag
     6241 aatacgattt ttattatccc gtcttttttc cgacatcgtt atgctccttt ccataatgga
     6301 aagagccttg atatgcttga ctacattgta ccacattcaa ggctcgattt cactattaga
     6361 ttgcgtccac cgtatcaatc attttttcaa attgctttcg cttaatctga atacggtttc
     6421 cgttcatcat tacccagtcc gggacgggat tttcttctgc cagtttacgc agcttacttt
     6481 ctccgatacg gaaatatttt gcagcttctt ctatggtaag ggtgtatttt tcccaaatcg
     6541 gcacgtcgtt attgttcatt ttgtcgtccc cctttctcaa tgggtcaaaa gtcaatcagt
     6601 aacataggct ttaatcggac ggctgttagc gcaacctcat gggacttgca cccctccggc
     6661 ggtctgccga agccctaccc attgcctgcg acgcttctaa cgctcggact atggctgtaa
     6721 ggaagtatca ttatgtattt tgtgtgttat cgcccgcagt ctgctatatc tgctttgcgg
     6781 cgtggtggtt ctcgctcgct tttccagtag ggggaaaggt catggcgcac ccgccgtatg
     6841 gctcggcaca aaaggaacgt atccgatttg ccttatgctg cgtcgccgtt ttcttgcgcc
     6901 gctttctttg tcaaagagca gggggctgtc ttgcggtatg gaacggaaaa ggggaaagaa
     6961 gcgttccaat atcgcatagg ttattcagcc cgaaaagtga ggatagccct catcagtctt
     7021 gcttgcagtc gttcccgaat atcctcgtca acgccataat agacattgcc gcgctcgtcg
     7081 tagagcttcc gcatggaaag acttgctatg tagccgctga aatgctgcaa gaccattttc
     7141 atagcgtccg ggtcgccttt gctcgccgcc aaaataacgg gatagggaac aagggcattt
     7201 tccgggtagt cgttttgatt accattcgtt ccattcatca gcgtgttcct ccaaatattt
     7261 ctttaacagt tcaaaagagc ttgtccgtct gtactgtatc gtgctgcgcg atgtattgaa
     7321 catctttccg atttccacat cagtcatgcc ctcgaaatag tacagaagta tggcagttct
     7381 cttttcttcc ggcagggtgc gtattgcttc aagcaataac ttcggggtta tttcttttcc
     7441 tgccatttga taagtcggct cggctgtttc agcttgaaaa tacttatccg tagtgtaaag
     7501 ctgccgttcc tcatgcaagg caaggtcaca aaaagaaact tcccgtttcc tgcgtcgccg
     7561 gatttcatca tgggcgttgc aggcttcgtt gtgcagcacc cgtttacaga aactttgaaa
     7621 ggcacattgc ttctgaaatt ctctgcgatt aggttccatg taatcacctc ctttctgccg
     7681 aaaggcgggt agtacctccc cttttcgcgg gataatacag acgacttttt caaacgccgg
     7741 acgaactgga aaatttattt gaaaaatctt ctgtaaagtg caaaatgcgc ctgcccgcaa
     7801 aaaggcgcgg acaggcgcaa aggaatagta aacagtattt agatgtattg atagttcata
     7861 ttcatagcca gacttacccg gtgggggaac tgtacttttt ttaatgaatg taacctatgt
     7921 atgctgtata gcaacaaaga aatccccata gcggcagccc tcctaaatgc cgctaattta
     7981 tgttataggt tgtcgtgatt tagcagtaga caaaaaaagc ggcggctcta aaaatcaaag
     8041 ccaccgcttc aaaggcgcgt gaaagtgtat ctgctctgcg acagcttttt ttcttttgcc
     8101 gtcgttcggc aggtactatc aatcaaatta tttaccaacc cttttttcta actgcttttt
     8161 tcttcttacc aacagtcaat gtaccgggtt ctttcttttt atttgcaaga ataatcatag
     8221 aaatcaagaa agtcaaaact attgtaatga aagcggggaa aatgtgataa gtataaccat
     8281 attccgtacc acaagtaggg tctactaaaa tcatgcttgc tttaatggga taataattta
     8341 acagtgttcc ttcaaaagtt ccaaaatatc catacacaaa agctagtgca actccaccca
     8401 aaaaattatt tgccgaacaa cagaatatca gaataatcgg caagactgaa atataaattc
     8461 ctatattcgc cccagtaaca cgaataaaca tattgagtag attgccaaaa tgaactcccg
     8521 gaaatccgac aataatattg attactaatg ctatcacaca acctataaga ctaaagctga
     8581 ttgtcaacag caatagaata agtaattttc cagaaagtaa ctgtttatat ggaataggaa
     8641 ttgttaatat attcttcata gtatcgtctg tatattccct tgttatgata taacctgcaa
     8701 tcagcgcaat aataatcggg aaaaataagg tacagttact aatcataacc tgttgcataa
     8761 aatagtcaaa cgtccatgtc tgtccaccgt tggctgtgga ataaaacagt gacagcaacg
     8821 gagaaagtgt tgtcataata attccggctt ttattacaga atatcgtttt aattttaaaa
     8881 attcagtttt tattatattt ctcatatctt ttacctcgtc cattttttat aaaagcgaat
     8941 tatcagccac aaagaaagta taactgtaat tgccataata aaaattgtat ggcaagtggt
     9001 tggtacaatc gcataggctt ccggcaaaag attatctttt tgcaaattat ccatcattaa
     9061 gccgcttgtc cagttcataa cacatataac cggaaaataa tttaagaaag caattttggc
     9121 agcacttata gatgtcccaa tcgttcccag tatgccccag ttaaaaatgg aataaaaaaa
     9181 tgcaagcagg atagaaagta aaaaactttt gttaaagtaa ataattaaaa atacaatcgg
     9241 taaagaagct gctacgataa gaactccaaa aataaggctc ataaatatct tataagtcat
     9301 accgtaaacc attcctacat gaaacaattt acaaaacaac gcaacagata atgtgcttat
     9361 cagacaaaat gcaacgctga aaataaagag catagaaatt tttgccataa tgagttgtgt
     9421 atttgtaact ggaattgttc taaggttttt ggaagtatca caatctcttt ctataaaaaa
     9481 caaaatagcg gcaatgatac caattagaca tggaagcaaa aaaactaaac catatcccaa
     9541 catcattgta taaacataat caaatcgggt ttgaagatag tgttccgttg tatctgcacc
     9601 ggttcccgcc tttgccatat atgccaaaat caacggaaaa aataacgaca atgacatcag
     9661 tgcatacaac agtctttttc gctttaattt ccaaaattcg cacttaatca gtttaagcaa
     9721 ttccttcgcc tcctgtcaca cgcttgaagt aatcttcaag actttcttca caagtatggg
     9781 cttctgatac ctccaatccg ttttctacaa aagcagtcac gatttttcct acggacaaat
     9841 caagattatg caagcgtaaa ttatggtcgt cctgtataga gaaatggctc tcatggaaag
     9901 tacgctctaa aattcttgct gcctgtgcgg tatcagaaag agtaaatcgg atatgtttgc
     9961 tgcttttttg ttccagttcc gcaagacttt cttcttccaa tagtgtgcca tggtcgataa
    10021 ttccaatatc atctgctagc agagaaatct ccgaaaggat atgactggaa attaaaattg
    10081 tttttccctt tgtatcgcaa agctcgcgaa taaaagaacg tacttctgca attccaatcg
    10141 ggtcaagccc attgataggt tcatctaaaa tcagaagttc agggtcgtgc ataactgcaa
    10201 gtgcaatcgc aagccgttgt ttcataccaa gagaatactg ggaaaacagt tttttgtctt
    10261 tataaggcaa tcctaccaaa tccaatgtgt ttttaattga atgacggttg ggtatgcccc
    10321 gtaaggtagc aaaaatttgt aagttttccg tagcagtcaa attagggtaa aaaccgggcg
    10381 actcaatcag actaccgata cggggcaaca atttcttttc attcccctgc aaagactttc
    10441 cccatatttt aacttcgccg gaagttggct ttgtcaatcc aagtagcatt ttcatagtgg
    10501 tcgtttttcc ggctccgttt cttccaagca gtccgtaaat tctccctttc tgcacatgaa
    10561 tatttaagtc ggcgacactc ttttgtgagc cgtactgctt tgtaagattt tttgtttcaa
    10621 tgatataatt tttgtccata ttcaaacctc ctgttctgta aagtattcta tcataccaac
    10681 cttgcattaa ccttgccgca accttgcatt aaccttgcaa tttgaaatat tgtcccaaaa
    10741 ataattaagg ctcccgtcaa aaggacggga gaaccttaat ttgctaaggg aaaaagcaaa
    10801 gtaaattccg ttcctgttcc ctgtatgcta tttgctgtta ttgtgccgtt cattttttct
    10861 acaagttggt gtgcgataga aagcccaaga ccgctgcctt tttcagaccg tcccttatcg
    10921 catttataga gccgttcaaa aatatgcttc aaatcttctt tttcaatccc tattccatta
    10981 tctgctaatc ggatttgcat atttttttcc tgctttgaca ggattatttc tatcttatcc
    11041 gcatgactgt gagaaattac attctgaata agattgttca atatccgcat atagccgtcc
    11101 gtatcaagtt ttaccc
//
LOCUS       DAEMIK010000141        11054 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEMIK010000141 DAEMIK010000000
VERSION     DAEMIK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.143"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..78)
                     /locus_tag="KPK87_003096"
     CDS             complement(<1..78)
                     /locus_tag="KPK87_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2539220.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILS"
     gene            complement(91..1287)
                     /locus_tag="KPK87_003097"
     CDS             complement(91..1287)
                     /locus_tag="KPK87_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2539221.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVVSGITREYLNKIESGK
                     MKPSKELLETLNKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDV
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(1471..2865)
                     /locus_tag="KPK87_003098"
     CDS             complement(1471..2865)
                     /locus_tag="KPK87_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2539222.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            complement(2959..3396)
                     /locus_tag="KPK87_003099"
     CDS             complement(2959..3396)
                     /locus_tag="KPK87_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539223.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(3498..3881)
                     /locus_tag="KPK87_003100"
     CDS             complement(3498..3881)
                     /locus_tag="KPK87_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2539224.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(3897..4223)
                     /locus_tag="KPK87_003101"
     CDS             complement(3897..4223)
                     /locus_tag="KPK87_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2539225.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(4349..7393)
                     /locus_tag="KPK87_003102"
     CDS             complement(4349..7393)
                     /locus_tag="KPK87_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2539226.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVADTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDRQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTLDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKPETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            complement(7636..9318)
                     /locus_tag="KPK87_003103"
     CDS             complement(7636..9318)
                     /locus_tag="KPK87_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2539227.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKVFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHQAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETCVLLQRRTM"
     gene            complement(9419..9571)
                     /locus_tag="KPK87_003104"
     CDS             complement(9419..9571)
                     /locus_tag="KPK87_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539228.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(10060..10470)
                     /locus_tag="KPK87_003105"
     CDS             complement(10060..10470)
                     /locus_tag="KPK87_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2539229.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
BASE COUNT         2904 a         2487 c         1792 g         3871 t
ORIGIN      
        1 actcaaaata cacatcaaga ctacgccgat acccattccc atagatacca gcaggaagtc
       61 ttttactaat tcccacattt tttatcactc ctaactttcc accaaatctt ttgcaggggt
      121 cgtctgctgt ttgattatca tttcatgctt ttcactcaag attgcctgct tttcaatcgt
      181 ttccatgtag tctgtaccgt ttcccttatc aatctttttc agcattttca aggtcggtgc
      241 tacctgtctt tgtacccaac gcaatgtgcg gtctaaggtg taaggttctg gttttgtcgt
      301 cagtttcaga ctttgccgat tatcgccaat aaaccaagcc cagcggtcat tgagtttcca
      361 gtcatttttc cgcttgtctg gttcttcatc aataaaccgc acatactggt tgatgataga
      421 aaaagcggtc tgctctgcgt catagtaggt cagcaaatct cgtactgcat agtaagcacg
      481 ttcatttcta agccgtatct caaaacggtt gataatgtcg gcttcttcca gaggtgtacc
      541 taatttgaca tactgctcat agtccttttc atagatacag aaatacacat ctgatttcag
      601 cgaaccaaga taaagggtac tccccatata ttctctgtcg tcctctctgt gcttgataag
      661 ctcgcccgac tggtaatact tatagcttct ggactttccg atatattccc gtttcctgca
      721 tttctccgca agctctggaa tatccaaaat gcccgtatgg tcgttgatag caaggtcgat
      781 acgcttcata atgcctccgt ctatgagtac gtccataaga aagtcatacc agcttctttg
      841 ctgtgccagc aggtaacttt caaactgcct gcaaccccga ccctttaact ctaaaaggac
      901 acctttttct tcgtcagccg acgtatagat aaagatgtcc cctaatgaat aatgctccgt
      961 ataactgtaa tgtccgtaat cttcatggag catataattg atattcagtt ttaatatatc
     1021 tttgatgatg tgctgtatat ccagcgtggg aaaacgaatt ttcacataat caaacagcat
     1081 ggtaagcggt gcttctggat tgaaccttgc cagttcctta ttcaaagttt ccagaagttc
     1141 ttttgacggc ttcatttttc cgctttctat cttgttaaga tattctcttg tgataccaga
     1201 aaccaccgcc agcctgcctt gtgagatacc gtaagcaatc cgtttctccc gtaattcttt
     1261 tatccattgt tcttcattca gaaccatacc cccaatctgt aaagtgtgaa gttttttaag
     1321 gcgacttcac agccgatttt tgctaggaaa ccgtgccaga agccttatgt ttcctatgtt
     1381 ttttgtcttt tgtctatttg caaacgtacc cctctgttag ataccgaggg gttttatctg
     1441 ctggcgtggc ttacgccaca ccagcaacgg ctagtccgtg ccgtcgcctt tcgcttcgca
     1501 cgtcgccgtc ccgtcctgcc ttgcttgtgc aagagagccg atagtctgta aaaaatcatg
     1561 tcctttcggg actaaaggcg tataaaattc acttataacg cttgttccca catcacaata
     1621 gccacgtccc ttgatacgct tctgaaaaaa ctgctttttc acatctgaac caaacagcat
     1681 accgtaacct aattcgctga tacgcccaag tcccacacgg aaattgaagt tatctctgat
     1741 accgtccgag aaatattttg cgtctggacg ctggcaggca acgataagga aataacctgc
     1801 ttgtcgtcct aacatgacga ttttctttaa ctggctaagt aagcccacac tttcttttgt
     1861 ccccagcatt tcaaaaaacg ccacatattc atcaaagata agaaagcagg gtggcagtcc
     1921 cagataggcg taattttcgc ccgtcttata gttcgggtgt cgtttcattt gctcgcttcg
     1981 ctgtaccatg ccctcataaa aagcattgac acagtctatc atttcttctt tggtgtgata
     2041 cacgtttccc atgactgttc ccaagtctgc taagtccgca tttttcgggt ctaagatgta
     2101 aaggacagcg ttggtatgca gtagggcttc aatgagcgtc agaagaaaat atgttttacc
     2161 gccaccagtc ccaccagcaa tcagagcgtg agggagtgcg tcatattccc agacaagatt
     2221 tttcatcagt ctaagacagc cgttctctgc ccgtacttca tcaatggtaa tgcggttcgc
     2281 tatcatatca taaagcaggg tatattcgat atagccgtca tgcagggtct tgtcggtcag
     2341 ctcacaatac aagccacttt ccaatttatc ctctaaccgt aaaagctggt cttgatattt
     2401 ccccagcgta atttcacagc ggatatgaag cagtcccttt tccatttgat aataaatctt
     2461 tggaaaccag acgatttttt cccttgatct gctttgcagg tcagtaaaaa aaacgctgtc
     2521 ttgtacggta tcagcttcat accacttgtt ttccagtatc attcgtgcca gcttttgacg
     2581 gtgcagtagc tttttgaaac tgtcgtaaca gaagcggtaa tacagaaaag cgaccaatgc
     2641 acaaacacca gtcgctatca gtatggttat gaagttgtaa agggaaagcg taaagccatt
     2701 gtctaataag ctgaaatgtt cccagtcggt acgcatgagc tgttttgtat ttaatagcag
     2761 gataaccgct acgaacacaa acaggagtgt ccccattgaa aagcgataga caaggttctt
     2821 atcgctggct ctgatacggt gtcctttgtt taaaatcatt ctcataaatc aatcactcct
     2881 ttctgggtat gaaaaaagca gtcaatcgta tagactaact gctttgtgtg catggatatg
     2941 aagttgtcaa actggaattt aacgttcact catatattgt tttatttcat cagaagtcaa
     3001 ttttcgtact tctgtatgtg gatattcttt atactctcca acattaaaca caggtgtcat
     3061 ttgtttgcat ttcttgtctt tattcttttt caagaataga tgtaaatcct cattcccggc
     3121 gaatatttta taagatactc tgcctttact gttctctatc tcataaatac cacatgaact
     3181 tttcgttgta taatcggctg ttcgtaaata aagttttgca atttccaatg acttaaaagg
     3241 aaaattccat ctttgtaatc ctctgtttgg gtcatgttca atacaaatgc aacgtccaca
     3301 agttccacaa atatattgag tgtggttttc taagcagtcc aagggattta gtaatggtgt
     3361 tattctattt tcatcagagt aacattcttc acacattttt cttttcgctc cttatcaaat
     3421 tctaatttgt cgctttcttc atacccatat tataacagtt ccgtatatcc tctacaacga
     3481 aaatctattc agcaagtcta ttttttcggt tgtccctgcg gtgtatggtt ctgtgaagtg
     3541 cctgtgttct gattgccttt attcttcagt acaatatctt ctgcctttac ataccagtcc
     3601 acatctgcac ccgtataggt ttttcgtgat accgtatcgg ctacgggatt gacaagctcc
     3661 acgactgcat tgtaaggaaa ttcccttaac ggtacttctg gcggtacgga cacggggata
     3721 attccaccat gcagactgca cttcaaatca tagatacgct ttttaagctg ggtactcggt
     3781 gtcccgtcct cattttgcaa gaacacatca cgcaccgctg taaattttaa ttctccaaat
     3841 gttttctctt tgtcaatgac aaatccattt gataatctca taaattcttc actcctttac
     3901 ttttcttcaa ctgctacaat atcatcagct actaatacat aatcggtatg tcccacatcc
     3961 ccgattgcgt accctctgcc gtataacttc ggattgacaa gttttacttt ctgttcatac
     4021 ttgaaatgct tttcgccagc ctgcacggga atttccacca caacattttc gcctttctgt
     4081 acgtcagaat aaaggttata gcttcgtctg gctatgacct tgcggttgtt tttatccctt
     4141 tcaaagatag gctcgctttc gcctgcaaat tcaagagttc caaaagactg tgccatatct
     4201 ggcacgacat atttcatttc catattgttt tacctcgttt ctttctatat ttgataagtt
     4261 tatgattgta attttttatt ctggcttgtc tgccgtttga cagtttcggc ggagaacgtg
     4321 atttttcttt catcataccg ttaccgcctt atgatttttt cattgtacgg cgtttgtaaa
     4381 gatatgttcc agccagtcca ccagcagatg taagcaacat cacaagaaat gccatgacat
     4441 ttgtgctgtc gcccgtttta ggactgtcgc taggtctgtt tggcttttct ggtgtctgcg
     4501 gtgtttcggg tttctctggg acttctggtt tttcattaaa agtaatcgtc tgtccctcgt
     4561 cctcaatatc cttatgctcg gtaactttct tcggctcgtc tggattgctt aaatcataca
     4621 attcttcaaa agttacaagc tgtttgccgt caagggaagt agcgtcaagg gtaaaggcaa
     4681 cttccgcttt catagtttcg ctgtcagcgg taaaggtgta atcactttcc acacgtttcc
     4741 cattgataag aagttctgca ttttcttctt tcaacatctg ccagcctaca agtttgtact
     4801 gtgtaccgac ttccaagccc tctaaggtta cggtatcaat gattgtaacg tcttttccag
     4861 cttcaatctc tttgttgccg tctttgtcgg tagcagtcgt atgtatcttg atgatacgtt
     4921 ctgtaatcaa tacagtttgt ctgtcgtcct caatgtcttt gtgttccgca actttcacgg
     4981 gttcgtctgg attgcttaaa tcgtataatt cctcgaatgt taccagattt ttaccgccaa
     5041 gctcggacgc attgaatgta taagaaattt ccactttcat ttcttcatca tcagcgataa
     5101 aagtatagtc attttccaca cgttttccgt caatgataag ctctgcattt tcttccttta
     5161 acatctgcca gcctttgagc tgatattttg taccttttgt aagcccgtct aatttgacag
     5221 tatcgacgat tgttacctct tttcctgcaa ggatagtctt ttcgctgtct ttgctggtcg
     5281 ctgtggtatg gatagagatt tctttttcgt attcatcagt caaagtccct aaatcaatca
     5341 caaggttatt tcttgatacc acgatttcaa aaggtgggat aagttcaaag cctttgttac
     5401 tatcgcaacg catttcttca atgatgtaag tatcataagg caatgcacct ttgctgtcgt
     5461 ctgcttcaga agttccaaac cacacaccgt cctcgctggt cttgcctgcg ttagtattgt
     5521 gcttatgaga agtccattca gcagaagtgg agaattgccc gttatcatca gtaactacaa
     5581 catgactttc tcccgtcgtc ttgcttgtga tcctaaaggg aacatcagca agacgcttgt
     5641 gtgtgcctgc accgattttc actccctcaa tatccccacg cttaatctga ttgtagatag
     5701 agtgggcttc gtcggttaag tccacgattt ttccattctc tgtgattgca aagtcaatcg
     5761 gcttcgcacc gtctgtcaag taaccgtcgg gagcttcact ctcaacgata cggaatttcc
     5821 cataaggtaa aaggtcagca gaagtagaag ccacaccctc aatatctgta cgaattgtct
     5881 ttaccacttc atttttctta tatagcttgc cctcaaccaa aacagaattg tcatttaagg
     5941 aaatgacgtc aaaagcagta tctttcaaag tggcacttcc ttgtggtttg gtatcgctcg
     6001 tttctaaatc tcttttctga attttcacac cgccacggat aaccttgtct gatacggaaa
     6061 actggttact tcccgtcaat acagcaaggt cgccgtcctc tgtaatttgt gtaaggtata
     6121 agccttttat ctgttcagac ttatcgccag cctgcatata tgcaccgtcc agcaagtagc
     6181 cgtttggagc ttttgtttcc tcaacagata gtgttcctaa aggaagaacc gctttgccgt
     6241 cctgcatata gaagctgtcg ccagatacct tgtaagcgtc cgctaatttt gtgatgtagt
     6301 gagttgtccc gtcgctgtct gtttcagcga ttgtctttgt aacccatgta cgagtagctt
     6361 cggcagggag attgtctttg ttatagaagc cagcataata cttccatgta aactctgctc
     6421 cctctaaaga agcgtttccc tgcggattgt ctttctgcgt ttccatgtca atcttgaaaa
     6481 gctcaatcaa ggtgtccgct acttttggtg tatctgatac gttcaaggtc gctgtctttc
     6541 cagcttcaac ttttaaggaa tacacggttg tatcaacttt atatcctgct ggtgcggata
     6601 attccctaat atagactgta cctgctttta cctctacaac atctgtattt ccgtttttat
     6661 cagtcgtaag ggtggcaagc tgtttcgtgc agtccttatc agaaaagaca ccataggtcg
     6721 caccagcgat tgagtaattc ccgttaccgt ctgtaatgct ggtattactg gaagtctttt
     6781 gcagttttgc atttccgaca tttaacttcg cccagaattg tcccaattcc tgcccctcgc
     6841 cagagtagat ataaccgcca cattcatagc gtcctttatt ttctttcaca aaggctttag
     6901 caccagcgaa aacttcgtcc tgcgttgctt ttggtatttc atcataagaa gctcgcacgt
     6961 tgtcgcattg ccagccaaga tgtacgctca atctctgcca gacaacacat tgttccaaca
     7021 gatagacttg cttgtagttc aattccttgt gtgcttcgcc atactgtttg acatactcta
     7081 aggataacgc cacatctgaa atctggtcgg cactcatacg ggtgcttgcg tcagctctgg
     7141 tcttgtagcc attcttaaaa tctgtattga tgtctataca gtaagcgtcc tcgccctcaa
     7201 cagtcaaatg tccctcgtta aaggtagaac caatcgaacc gtcattcatt actttttcta
     7261 cgataccgac acgttcctct gattccgtcc agtattgctt gctttctgca tggacggcgg
     7321 tactcggtaa agcagtaacg acagtcgcaa aggcaagtac tcccgtacac aatcgtttca
     7381 atatcttttt cataattcta aatgctcctt tcattttggg taaaaaaata gacgctcaat
     7441 attgaacgtc ttacattgaa aagggatact ttttacaagt acccctcaaa tctaatattt
     7501 aattgtttgt gttcccacac tctatatcaa caactactga aacattgata tagtttttta
     7561 tctctttatt tctgatttta tcgccctcgc tcgtaaagac tatatgctta aaaaagtgcc
     7621 taaaattaag gatttctaca tagttcgtct ttgtagcaac acacaagtct caacttgctc
     7681 atcattttcc aaacttatat taagatcttc atcaatgata ggtagcttaa aagtaatgga
     7741 ttttagccat tgtccattag gctgcttttc ttcataaatt tgaatttctg aaatgagtgc
     7801 ttctattaac tgcctgcgtt caacatcgtt catgacttta taaagcttgt caaaataaat
     7861 caatacttta tatatgttat caccagtaag tttctcaacc tctatcgttt ctttttttgc
     7921 tttagcttct attaactgac tttctaattc ctcaatttta tcgtacatac gataaagcct
     7981 atcatctaaa tcattttttc ttctcttata atgtttatcc tctacatcta aattatctat
     8041 ttcctcaata agcttatact ttgtagaatg actttttctt agctcatttt ggtaattatc
     8101 tatctcattt tcaatagcag atgtatcaac cttcatgtta attttttctt gtatcataga
     8161 tgcaaattta gggttgctta ctaacttgat tattacctca gcaacggcat catctaacaa
     8221 ttcttctcta atttgtttat tataggtaca tttatgtcct ctattcataa gtctatgctt
     8281 acaaccatag taatagaagt ctttatactt cgtcccatct tttttatatt ttatagattt
     8341 attcccaaac attccagctc cacatatcgg acactttact attcctgata ataagtgtgt
     8401 cctcatgttc ttgcccttat ttacatgttc atacttttta gcttgtgctt tcagtttaac
     8461 ttgtgcagcc tgccacaact catctgggat tattgcttga tgaatgcctt ctgctactaa
     8521 ataatcgtcc tgctctactt gtctgtattc attccttgta ccatgaactt tttctaaagt
     8581 tcttcttcca aatgctattt tcccgttata cacaggattc tttaatattt ttcttataag
     8641 acctgcgtca aacaatggat ttttcccatt ttgtcttggt attttcctta taccatgatt
     8701 ttctaaatat ttagaaagac cattagctcc aatacttgta tttacatatt gatcaaaaat
     8761 agttcttatt gcaattgctt cttcctcatt tattatcaat ttcccatcaa taagttgata
     8821 accatacgga gcaaatccac cattccattt accctctttt gccttttgaa tgcgaccttc
     8881 cattgtttgg acacgaatgt tttccctttc tatttcagcc actgccgata aaacagaaat
     8941 catcaattta cccgcatctt tagatgaatc aattccatct tcaacgcaaa taagatttac
     9001 tccaaaatcc tgcattattt gtaaagtcga aagtacatca gcagcatttc ttgcaaatct
     9061 cgatagttta aatacaagaa cgaaagacac tccatccttt ccggatttta tatcttccat
     9121 catacgagta aactgaactc taccttctat agattttcca gacttacctg catcttcgta
     9181 ttcaccaaca atctcataat cattatagag agcaaaaacc ttcattcttg atttttgtgc
     9241 ctctaaagaa taaccatcta tctgtatgga tgtagatact cttgtataga gatatacttt
     9301 tattttttct tttgacatag cattaacctc aatatcctta ttatatattt atatattttt
     9361 accaataaaa gtttggactt taatctcaag tatattatag cacttttatt tctctttctc
     9421 aatccgtgct tttagctata tcaaatttat tcttatcctc tgtatttttt actggagtag
     9481 gatttggaag attctctaaa tctaaaactc cagcatattt tgtaatcaga tttgctatca
     9541 aatcagccaa tccattccag tcattatcca ctaatacacc tcctccactt taaattcttt
     9601 ataaccaaat attatttttc ttactaagtt ttatgacatt ggtaggtgca ttggctgcta
     9661 cataggaatc tcacctcctc cccttgtttc agacgaggca gcttaaacta ttgaagtatc
     9721 attatcacca cttacatcat cgaaataagc tgccacgctt atcttttatg tattcctatc
     9781 tatcgctcat tttcttctaa cctcggcatt atgccggtat tcatttgaac cgattttcat
     9841 cagcagaaaa gtcttggcgt aggtcataac tctcagtaag tagataaagt cctaccttgg
     9901 tctattcagt tgtcaaagag caatatttga aagggactgt tttctttaca ttttcctatt
     9961 tgccataagc aggcgtatct cctactcaag aaagtaaagg agatcaaact ccccttcact
    10021 ttacataagg aaaatttgcc ccctttggtt accgaagttt taaagttctt cgataaattt
    10081 tttcagcttt tcaagaattt tgtttcttct tttgattaaa gccggatgtg taatactttt
    10141 gagctttgct actgaacgaa cagtttcatc attgaaatac aagcgttcaa taatatctct
    10201 ttcttctgca ttgagccttg ttatagcact tcttactgct tcaatcatca tctgtgtttc
    10261 aacaatcttt tctacatcaa cactttcatc aacaatatta tctgtaaagt gtccgtcatg
    10321 atccaatgct gaaaaaaaga gcaagtggtt tttcctgtcc acctgctcta aatacttctc
    10381 gtgttctttt tctcgccagt agactatata tatatcttca ctgactttta ccttttgtcc
    10441 tctgatataa agataatact cttttgccat aaagtttcct ccatctcttt tttatctgct
    10501 tgtttttcag ataaaaaagg aggacttctg catcttggca taagaagtcc tctgaaatga
    10561 aagaataaaa atatttcttt atatgaactt gattgtttca attcgcacct gaaacaatca
    10621 tctactatac atattataaa aaagaggaat atattgaatt ctaaatattt ctaacattaa
    10681 acgtttagat gcttccatat ttatttcctc cattttatca ataatctaaa taaccatcat
    10741 attcatctac tacttatata attgagtttt attaaacttt ttaagtttct attctatcaa
    10801 atatttaaac aatctctgca tccgaaaaga ctaactcata tagttttact aacaatattt
    10861 ttattagcgt gttcaaatcc tgatttcttg tatcaattat tttctgttca ctttcaataa
    10921 gaaaaaagcc tttacttcca tggctaataa taagtctcta tttttcaata actattgcaa
    10981 aattatatct tgtttaagaa aatattttaa ttttaaagat aaaaaattct tcatcctgca
    11041 atgctttata ttct
//
LOCUS       DAEMIK010000142        11031 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEMIK010000142 DAEMIK010000000
VERSION     DAEMIK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.144"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            279..1601
                     /gene="dnaA"
                     /locus_tag="KPK87_003106"
     CDS             279..1601
                     /gene="dnaA"
                     /locus_tag="KPK87_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG2539230.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1843..2949
                     /locus_tag="KPK87_003107"
     CDS             1843..2949
                     /locus_tag="KPK87_003107"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG2539231.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            3086..3292
                     /gene="yaaA"
                     /locus_tag="KPK87_003108"
     CDS             3086..3292
                     /gene="yaaA"
                     /locus_tag="KPK87_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG2539232.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3310..4425
                     /gene="recF"
                     /locus_tag="KPK87_003109"
     CDS             3310..4425
                     /gene="recF"
                     /locus_tag="KPK87_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG2539233.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4426..6327
                     /gene="gyrB"
                     /locus_tag="KPK87_003110"
     CDS             4426..6327
                     /gene="gyrB"
                     /locus_tag="KPK87_003110"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG2539234.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMIAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6347..8773
                     /gene="gyrA"
                     /locus_tag="KPK87_003111"
     CDS             6347..8773
                     /gene="gyrA"
                     /locus_tag="KPK87_003111"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG2539235.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8842..9138
                     /locus_tag="KPK87_003112"
     CDS             8842..9138
                     /locus_tag="KPK87_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2539236.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            9138..9398
                     /locus_tag="KPK87_003113"
     CDS             9138..9398
                     /locus_tag="KPK87_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG2539237.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9410..9727
                     /locus_tag="KPK87_003114"
     CDS             9410..9727
                     /locus_tag="KPK87_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG2539238.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYILNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            9752..10138
                     /locus_tag="KPK87_003115"
     CDS             9752..10138
                     /locus_tag="KPK87_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2539239.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            10138..10911
                     /locus_tag="KPK87_003116"
     CDS             10138..10911
                     /locus_tag="KPK87_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG2539240.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4490 a         1146 c         2008 g         3387 t
ORIGIN      
        1 ttagaaatta ttcacagctt gtggatactt ttgtgtataa cttttaagat tactgacaat
       61 actctagtta taatcaccat ttattgtatg tttatgttat ttataacttt atctacatat
      121 tgtttatatc ttttcatatt tttttaataa tttatactat tattaattta tcaacaccat
      181 tatccataaa tttagttatt ctgtaaaatt tatatatatt tttgcttttc tgttaataac
      241 tttgtggaaa atattaatta ttctgttcgg aggtttatat tatggatata gtttctttat
      301 gggacaaaac cctacaatta ataaaaggtg acttaacttc agtaagtttt aatacctttt
      361 ttaaaaacat cgtaccatta aaaatacatc ttaatgattt aatcttactg gctcctagtg
      421 attttaataa agatatctta gagaatagat atttacattt aattgaagat gctattagtc
      481 aactgtcatt aaaaaaatat aatattaaat ttgtattgag cgaaaaagag gttgctgatt
      541 taaattctga tagcacagat ttaaattata gagttcttta ccccaatcta aatccaaaat
      601 acacatttga tacattcgtt attggtaata gcaatagatt tgcacatgct gcatgcgttg
      661 cagttgcaga atctccagct aaagcttata acccactttt tttgtatgga ggagttggat
      721 taggtaagac tcacctaatg catgctatag gtcatcatat tgttagtcaa aaaaaagact
      781 caaaagtagt ttacgtttct tcagaaaaat ttactaatga acttataaac tctataaaag
      841 acgataaaaa cgaagaattt aggaataaat atagaaacgt agatgtatta ctaatcgacg
      901 atattcaatt tatagcagga aaagaaagaa ctcaagaaga gtttttccat acattcaata
      961 ctcttcatga agcaaacaaa caaataatta tatctagtga tagaccgcca aaagacatac
     1021 ctacactaga agataggcta agatcgagat ttgaaatggg acttataact gatattcaag
     1081 cacctgactt tgagacaaga attgctatac ttagaaaaaa ggctcaacta gaacgtatag
     1141 atgtgccaaa tgaggttatg tcatatatag caaaaaatat taagtcaaac ataagggaac
     1201 tagaaggtgc attaactaga gtggttgctt attcttctct tagcaatagg gtaatatctt
     1261 ttgatcttgc tacagaagct ttaaaagata ttataacaac ttctaaaaat gaagaaataa
     1321 atgttctcag aataaaagaa aaagtatctt ctgtattcaa tttaaagatg gaagatttta
     1381 attctaaaaa aagaacacgt tcaatagctt atccaagaca gatagctatg tatttaacgc
     1441 gcgagcttac tgatttatcc cttcctaaaa taggtgaaga gtttgggggt agagaccata
     1501 caacagtaat tcatgctcat gacaaagtat ctaaggatat agaagaaagt gaagaaatta
     1561 agactaaaat agacaaaatt atatcagatt tgaagggata atttatacac aattgtatgt
     1621 ttatatctct tgaataacct gttaatactt tttttgaaca aaaattatta acaaattatc
     1681 aagtctaaaa taacaaagat attaacatct tatacacatg ttaatatctt tgtatttata
     1741 atgttataca tacttattaa cattatcaac aacacctact actattacta ctatgatttt
     1801 aacttatttt tatatataaa aagccagagg aggttatcca cattgaaaat aatttgtaat
     1861 caaaaaatcc tagcaaatag aattggaatt gctcaaaaag ctattaatgg aaaaacaaca
     1921 atagaattat taaaaggtat acttatatct acagaagaag gtcaattaaa acttactgga
     1981 tatgatgctg aaataggaat agaaacttat gtacaagcag aaatcataga aaaaggtgat
     2041 gttgttgtag atgcaaggct atttggagat ataattagaa aattaccaga ttcctttgtt
     2101 gagatagaaa ctgattcaga aaataatatt tacataaatt gtgtaaattc aagatttaaa
     2161 attaaaggtt atgcagcaaa agaatttcct aaattaccag aattaaatga agaagattta
     2221 tatagcatac ctcaagaaat cctaaaaaat atgattaaac aaactgtatt tgctatctca
     2281 caggaccaaa caaaacctgt tttaatggga gaacttttag aaattgtaga tagaaactta
     2341 aatttagtag caattgatgg atatagatta gctgtaaaaa gttgttcagt tgatagttta
     2401 actgaaaata taaaagtcat aattcctgga aagacactta tagatgtaaa tagtttgctt
     2461 tctggagagg ataatgttaa agttggattt aacgaaaaaa atgctatttt tataattaat
     2521 gatacgaaaa ttattacaag attgcttgaa ggagatttta ttgattataa aaaattattg
     2581 ccaagagaac ataacagcag agttaaatta aatactaagg aacttttaaa tagtatagag
     2641 agagcgtctt tattgtctca atcagaaaaa aataatctta taaagttatc tataagagat
     2701 aaagtaatgg caattacttc taatacagaa aaaggaaatg tgtatgaaga agttgaaata
     2761 gatttagatg gagattacct agatatagct ttcaattcta gatattttat cgaaggtttg
     2821 aaaaatatag ataatgagga aatatttata gaatttacta caaatgtaaa tccttgtata
     2881 attaaaccaa cagatgatgt taattatatt tacttactgc ttccagtaag aatatcatca
     2941 aatatatagc agacatgaag gagttgtctt ttgtagaaaa tttttctaat cttagacaac
     3001 tccttttata agcatattta tggtataata tacttttagt gtatcaatag gaatttgttt
     3061 aaaatagtga caaggaggaa ctattatgac tgaaataact atagaatcag aatatataaa
     3121 gttggatcaa tttcttaaat tagcagagat tgcatcaaca ggaggtcatg caaaattttt
     3181 aattcaagaa ggtttagtga cagtaaatga tgaaatagaa ttaagaagag gaaaaaagat
     3241 aaaatcagga gatatagttg aaatagaagg aactaaaata aaagtattgt aaaaaaagga
     3301 gaactttttg tgaaacttaa aagtttgcaa cttgttaatt ttagaaatta taaaaagttg
     3361 catctggaat ttaatgggaa agtaaatcta cttgttggta aaaatggtca aggaaagaca
     3421 aatatagtag aatctattta tatgctttct tttggaaaat catttaggac taacaaagat
     3481 aaagaaatgg ttagatttaa tagtgaaaac ttatatattg gaggaagttt ttcaaaatat
     3541 aataaatata gtctaataga gctaattatt ggaaaagata aaaaagggat aagaataaat
     3601 aaagttcctt tacaaaagat acaagaacta cttggcaact taaatgttgt tatattttct
     3661 cctgaggatt taaggttggt taaagagggt cctaaagaaa gaagggcatt tatagacaaa
     3721 gagattagtc agattatacc taaatattat aaatatctta ccaattataa taaaacactt
     3781 tctcaaagga gtagggtgct taaaaatata catgttgatg aagctttatt agatgtatat
     3841 gatgatacgt tagcaaagta tggaagttat atttatattt tgcgtagaga ctttataaaa
     3901 aagatagcta atatatctga aaatatgcat atgaatttga ctaatggagt tgaaagatta
     3961 tcaattcgat ataaaaatca aataaatata actgatgaag atactataga tacagtatat
     4021 aataaatttt tagccaagtt gtcatcgaat agacctaatg atatagaatc taagactacc
     4081 agatatggta tacacaaaga tgacttaaat atattcataa atgatttaga tgcaagactt
     4141 tttggttcac aaggtcaaca aagaactgct tcaatatcat taaagttatc agagatagaa
     4201 ttaataaaaa atgaagttga agaatatcca gtattaattt tagatgatgt ttttagtgag
     4261 ttagatgaag caagacaaaa acttttagta aataatttaa gcaatgttca aatgtttata
     4321 acaagtgctg aagtttcaca taaaaaaata tttgatgaaa aaaatgttac aatatttaat
     4381 attgaaaatg gcgatgttat tagcatagag aatggaggaa attaaatgaa acaagaatac
     4441 ggtgcaagtc agatacaagt attagaggga ttagaagccg ttagaaaaag accaggtatg
     4501 tatattggta gtacaagccc tagaggttta catcacttag tttatgaagt tgtagataat
     4561 agtatagatg aggctttaca aggatattgt tcagacatat atgtatctat taatgaagat
     4621 ggcagtgttt tagtaaaaga taacggaaga ggtatacctg ttgaaataca tcctaaaact
     4681 ggtaaatcaa ctttagaaac agtattaact aatcttcatg caggaggaaa gtttggaggc
     4741 ggaggatata aagtatctgg aggtcttcat ggagttggtg tttctgtagt taatgcattg
     4801 tcaaaatgga tgatagcaga ggtttattta aacggaaaga tatataagca gacatatgag
     4861 aaaggattgc caacatctaa gcttgaagta gttggagaat ctcaagataa aggaacaatg
     4921 attcaattta tgccagatga gacaatattt gatgaaatag agtttaaata tgaaacattg
     4981 gagtataggc ttagagaact ttctttcttg aataaaggaa taaaaatagt atttgaagat
     5041 aaaagagaag gacaagaaaa aagaaaagaa tttcactata caggtggttt ggtagaatat
     5101 attaagtatt taaataaatc tagaactggt atacatgatg acatagttta tatagataaa
     5161 aaggttgatg attgttttgt tgaactagct atgcaatata cagatggtta tacagagaac
     5221 atatattctt ttgcaaacaa tataaataca catgaaggtg gttctcattt aagtggattt
     5281 aaagcagctc ttactaaaac tgtaaatgat tatgctaaga gaaataaatt tttaaaggaa
     5341 aatgatgtta acctacttgg tgaagacata agagaaggtc ttacagcagt agtatcagtt
     5401 aaattaccag aacctcaatt tgaaggtcaa actaaaacaa agctaggtaa tagctttatg
     5461 agaggtatag ttgatagtgt aacagttgat gaactggggt ctttcctaga agaaaaccca
     5521 tcaacagcaa gaatcatagt tgataaagct ttaagagcac aaagagctag agaagctgca
     5581 aaaaaagcaa gagaattaac aaggagaaaa agtgtattag aaagtacatc tttacctgga
     5641 aaacttgcag attgtgcaga aaaagatcca tctaaaagtg aaatattctt agtcgaaggg
     5701 gattcagcgg gaggttcagc taaacaaggt agagatagaa atagtcaagc tatacttcca
     5761 ttaagaggga aaatacttaa tgttgagaaa tcaagactag ataggatact atcttcagat
     5821 gaaataaaaa atatgataac agcttatggt tgtggtattg gagaagattt tgatatagat
     5881 aaggctaggt atcataaaat tataattatg accgatgctg atgtagacgg agcccatata
     5941 agaactttat tattaacatt cttctttaga tatatgagac ctcttataga tgaggggtat
     6001 gtttatgcag cacagccacc tttatataaa gtgacaaagc aaaagaagga acattatgtt
     6061 tactcagata aagagttaaa tatcttacta gatgaaattg gaagaaatgg tgttgaactt
     6121 caaaggtaca aaggtcttgg agagatgaat gcagagcaat tatgggaaac aactatgaat
     6181 cctgagacaa gaactttatt acaagttact gtagaagatg ctgcaattgc agatgaagta
     6241 ttttcaatgc ttatgggtga taaggttgct ccaagaaaag aatttataga agaaaatgca
     6301 agatttgtta gaaacttaga tatatagaga gaggggatta gtatacatgg aagaaaataa
     6361 caaaatactc cctattgaaa tagcggaaga aatgaaaaaa tcgtatattg attattcaat
     6421 gagtgttata gctggacgtg ctcttcctga tgttagagat ggtttaaagc cagttcatag
     6481 aagaatatta tattcaatga gtgagttaaa tttaactcca gataaaccat acaggaagtc
     6541 agctcgtatt gttggggacg ttttaggtaa gtaccatcct catggagata ctgctgttta
     6601 ttatgctatg gtaagaatgg cacaagattt ttcaactaga gcacttttag tagatggtca
     6661 tggtaacttt ggttctgttg atggggattc accagctgct atgcgttata cagaagctaa
     6721 aatgagtaaa ttatcattag aactactgag agatattgaa aaggaaactg tagactttaa
     6781 accaaacttt gatgagtcgt taaaagagcc ttcagtattg ccagctagat atcctaattt
     6841 attagtaaat ggctcaaatg gtatagctgt tggtatggca acttcaatac ctccacataa
     6901 tttagcagaa gtaattgatg caactgtata tttgatagat aatccagagt gtagtgtaga
     6961 tgatttaata aaatttgttc aaggaccaga tttccctaca gctgcaatta taatgggaaa
     7021 agaaagtata gcagaagcat acagaactgg aagaggaaaa gttaaagtta ggtctagagc
     7081 ttttatagaa gagctaccaa aaggaaaaca gcaaataata gttacagaaa taccttatca
     7141 agtaaataag gctaaactgg ttgaaagaat agcagagtta gttaaagaaa agagaataga
     7201 aggtatatca gacctaagag acgaaagtaa tagaaatggt atgagaattg ttatagaatt
     7261 aaagagggat gctaatgcta atatagtatt aaataatttg tataaacatt ctcaaatgga
     7321 agatactttt agtataataa tgcttgcact tgtagatggt cagccaagag ttttaaatct
     7381 taaacaaata ttatatcatt atattaaaca tcaagaagat gttgttacaa gaagaactaa
     7441 atttgaacta aataaagctg aagcaagagc acatatttta gaaggattaa agattgcttt
     7501 agataatata gatgctgtta taagcttgat aagagcttca aagactgggc aagaagctaa
     7561 gctaggttta atagaaaaat tcaaattaac tgaaatccaa gcacaagcta tattagatat
     7621 gagacttcaa agacttacag gtttagaaag agataagata gaagctgaat atgaagattt
     7681 aatcaagaag ataaatagat taaaagagat tttagctgat gaaagattac ttttaaatgt
     7741 aataaaggat gaaattacaa taataaaaga aaattactct gatgagagaa gaacagaaat
     7801 aagacatgct gaaggcgaaa tagatatgag agatcttata agtgatgaag aaatagcaat
     7861 aactcttact cactttggat atataaaaag gcttccatct gatacttata agagtcaaaa
     7921 aaggggtgga agaggtattt cagcacttac aactagagaa gaagattttg taaggcactt
     7981 ggtaactaca actactcata gtagactatt attctttaca aataaaggta gagtatttaa
     8041 attaaatgca tatgaaatac cagaaggtaa gagacaggct aaaggtactg ctatagtgaa
     8101 tttacttcaa ttgtcagcag atgagaaaat tgctactcta atacctattg atggtaatga
     8161 tgaaaatgaa tatttattac ttgcaacgaa aaaaggtatt gttaaaaaga ctaagagaga
     8221 agagttcaaa aatataaata aatctggtct tattgcaata ggtttaagag atgatgatga
     8281 gcttattgga gtagaactta cagatggaaa acaagaagta cttttagtaa ctaaagaagg
     8341 tatgtctata agatttgatg aaaatgatat aagatatatg ggtagaacag caatgggtgt
     8401 aaaaggtata actttaagta aagaagattt tgttgtgtct atgaaccttt gcagtaaagg
     8461 tacagatgtg ttagttgtaa gtaagaatgg ttttggaaag agaacaaata tagaagagta
     8521 tagaagtcaa ataagagctg gtaaaggaat taaaacttat aatatatctg aaaaaactgg
     8581 tacaattgta ggtgcagata tggtcaacga agatgatgag ataatgatta taaattctga
     8641 tggagttctt attagaataa gagtcaatga aatatcactg tttggaagag ttacaagtgg
     8701 tgttaaatta atgaagacga atgatgaagt caatgtagtt tcgattgcca aaataaatat
     8761 tgaagaagaa taggataaat atcctattct ttttgtataa tgtagttaga taaaaattta
     8821 aaattgaaag gagtaattgt tatgtgcaat aaaagaagtt gtaaaaaagg tggattttta
     8881 ttattaggag ctattttagg gtttatattt ggtatgtttt ttgcacctaa aaaaggttct
     8941 gaactaagaa aagaaacaaa agagaagttt aatgatgtca aagaaaatcc aaaagaagtt
     9001 ctacatgaaa catttaatga tgttaaagag agaataataa atttagtgga tgatgataat
     9061 aatgaagaag atataaagat ttcagaagaa gatatagtaa taagtaaaag ctttgatgat
     9121 gagggagatg ttaactaatg aatgcatggg ggtggcaaat tggagcagtg ttagtaggaa
     9181 gctctgctct tatagtagca atttatttag ctaaaactct aaacagcata aataaggttg
     9241 ttgaaaaggc atataagatt gtagattata atgaaagaca tatacaagat attgttgaaa
     9301 atgcatcatc aatatcaaag ggaatagatg atatagttta tgtaattaat aaaatattga
     9361 gtataggaaa tatatttaaa attattaaaa ggaaatagga ggtttttaca tgtctatgaa
     9421 tattgattca aatttagatt cacaaaataa attttggaat gtttgtcttg atggagaact
     9481 tgatgtttct acagcagata aactaaagga acatttacat gctttaatag aaaaaaatat
     9541 gttagatgta aagattaatc ttaaagattt agattatata gactcaacag gcttaggagc
     9601 tatgataggt gtattaaaaa aattaaaaat aaatgaaaaa gagatataca tattaaatcc
     9661 taagagtaac gtaagaaaga tttttactat aacaggtcta gataaaatat ttaaagtgga
     9721 gggataaact ttggcttgtg agactataaa gatggaaatt actacaaatc cagattatgt
     9781 tagtataata agattaacag catctggaat tgccaataag atgggttttc caatagatga
     9841 tatagaagat ataaaggtag cagtatctga agcatgcaca aatgcaataa aacacagtaa
     9901 agatgatgtg tttaatatag tatttaatat tttagataat gcaattaata tagaaattca
     9961 agataatgga aaaggatatg atgttagctc aataagtaca ccagatttag agaatccaaa
    10021 agagagtggt cttggattgt ttataattaa aactttaatg gatgatgtag atattgaatc
    10081 agaacataat caagggacaa aaataaaaat gactaaatat ttaggagttg atatttaatg
    10141 aaaaatgtag ctaatgctac acattaccta aatatggata caaaagagct atttaattta
    10201 tataataaaa ataaaaatgt agacattaga aatattctta ttgaaagaca tctgtattta
    10261 gcaagattgt tagctaagaa gtatataaac aaaggtgttg attttgaaga tatatatcaa
    10321 gttgcttcat tggcattaat ttatgctatt gatagatatg atgttgagaa aggatttgag
    10381 ttctccagct ttgcaacacc aactatagta ggagaaataa aaaagtattt tagagataaa
    10441 gtatggacac ttagagtccc aagaagaata caggaattaa gcaaaaagat aagtgatgcg
    10501 aagattaaat tagaacaaga aaataagaag catccaaagg taaaagatat agctgattat
    10561 ataggcgtta gtgaagaaga tgttttagag gctatggagg cttcttatgg atatcaacca
    10621 atgtctttgg attcatcaag taatgatgat tcagaggata aagatataac tcttattgat
    10681 aagattggaa aagaagaagg taactttgga agtatagagt atgaagattt tattaataag
    10741 tttattgaaa ctttaaatga attagaagtt aaaattttta aagatagatt tttctttgat
    10801 aaaactcaat ctagtatagc taaagaacta gggatatcac agatgactgt ttctagacta
    10861 gagaaaaagg tagtagagaa gttgaaaaaa gaatatgaaa aaaatttata aaaaaatata
    10921 aaaaaagtat tgacctactg ttttaaagat ggtatagtat tacttgtcct tgaaaaaagg
    10981 aacaagaaat aaacacgaac attgaaaatt aaacagtagg ttaatttata t
//
LOCUS       DAEMIK010000143        10908 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEMIK010000143 DAEMIK010000000
VERSION     DAEMIK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.145"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(82..816)
                     /locus_tag="KPK87_003117"
     CDS             complement(82..816)
                     /locus_tag="KPK87_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539241.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(785..1798)
                     /locus_tag="KPK87_003118"
     CDS             complement(785..1798)
                     /locus_tag="KPK87_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2539242.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDDRLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(1820..2590)
                     /locus_tag="KPK87_003119"
     CDS             complement(1820..2590)
                     /locus_tag="KPK87_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539243.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(2565..2855)
                     /locus_tag="KPK87_003120"
     CDS             complement(2565..2855)
                     /locus_tag="KPK87_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG2539244.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(2986..3085)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      3705..3794
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3847..4023
                     /locus_tag="KPK87_003121"
     CDS             3847..4023
                     /locus_tag="KPK87_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539245.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(4138..4494)
                     /locus_tag="KPK87_003122"
     CDS             complement(4138..4494)
                     /locus_tag="KPK87_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539246.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLEIDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            4750..5662
                     /locus_tag="KPK87_003123"
                     /pseudo
     CDS             4750..5662
                     /locus_tag="KPK87_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            5893..6504
                     /locus_tag="KPK87_003124"
     CDS             5893..6504
                     /locus_tag="KPK87_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG2539247.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            6802..7140
                     /locus_tag="KPK87_003125"
     CDS             6802..7140
                     /locus_tag="KPK87_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG2539248.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(7546..8982)
                     /gene="miaB"
                     /locus_tag="KPK87_003126"
     CDS             complement(7546..8982)
                     /gene="miaB"
                     /locus_tag="KPK87_003126"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG2539249.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(9230..10666)
                     /locus_tag="KPK87_003127"
     CDS             complement(9230..10666)
                     /locus_tag="KPK87_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG2539250.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT         3767 a         1670 c         1219 g         4252 t
ORIGIN      
        1 catataacta aaaacaatcc aaaatgtata ctcacatttt ggattgctaa gtttaatgct
       61 tattaaataa aaatattttt tttacaataa agatattatt tcttttttaa gtttaatgat
      121 attttcaagg ttatcttcat ttaactttct actatctata acaaattctt tctttactat
      181 tgatggtttt tttgaaataa cataaattct gtttgatagc attatagctt cgtcaatatc
      241 atgagttata agaagtattg ttgaatttac ttttttctta acatctaaaa gccatttttg
      301 catggatgtt ttagttaatg aatcaagtgc cccaaaaggc tcatctaaaa gcattatgtc
      361 attagaattt ataaaagttc tcaagaagtt agccctctgt ctcattcctc cagaaagttc
      421 tgaaggatat ttatcttcat atccgcttaa tccaaacacc tcaaaataag gttttaccat
      481 tgacctagct ttactctttt tttctttttt taaaattagt ggtagggaca cattatctat
      541 tatagtttta tatggcaaga gtaaatcttt ttgatacata taactatact taccatttat
      601 attcactttg ccactatcat aatctgtaag tttagttata atattaaaaa tagtactttt
      661 tccacatcca cttggcccta atatagaaac aaactcacct tcataaacat caatacttat
      721 atcttttaaa acttcaatat ctttataagt ttttgataca ttttctatag atacttttac
      781 ttttttattg tggtaaaaat tcatttgtgt aagcatcact tgccttcata tccttatcta
      841 ttaatttata ttcttttaag aatgatgtat agttgttcca tacactatct ttcatttctc
      901 cccatcgtgg tgcatcatct ttgtacttgc ttgctaaata ttcttgactc tttaaagcta
      961 gtttttcatc tacttcaggt gcatgtttta ctaaaatttt tgctgattct tctggatttt
     1021 ttattgcata ttcataacct tttgttgtag cttttaagaa ttttttagca agctctggat
     1081 tatttttaag cacagtttca ttagaaatta taagtggtgt ataataatcc aatctatcat
     1141 ctaaatcttt tactggtata aagtttaagt catatccaat taaatcagct ttaactgcat
     1201 cccaaccttc aaatatccat gcaaaatcta cagttttcat agctgcaaag aaatcatctt
     1261 gaccagtatt aactatcttt aacttattaa aatcagctcc atttttcttc ataactgctt
     1321 taaaaacagc ttcttctgat ggagagcccc aaccaccata agtttttcct tcaaaatctt
     1381 tagctgttgt tatattcttt tcttttggtg atgcaaaccc agaagtatta tgttgtataa
     1441 cagttgctac agcttttatt ggaagtggat cttcactagt ctttgcataa gtaacttgtt
     1501 cttgatagct tataccaaag tcagcttttc ctgttgctac taaagtttct gctccacttt
     1561 caggtggttg tactatttca acatccaaac cttcttcttt atagtaaccc ttatcaagtg
     1621 ctacaaataa tccagtatga tttgtatttg gtgtccaatc aagcaccata gttacttttt
     1681 tagaagaagt atctttatca ttgtttttgt ctttattgtt atttgaagaa catccagtta
     1741 ttgttgccaa tgtaaaaaca cttactaaac ctaatattcc taacttttta aaattcataa
     1801 ttgcctccct caaatacctt taatctatta atctatgtct tataacaact ttttttacag
     1861 cctgtacaat tcctacaaac attaaactaa atataactac taatatagta gatgcaaata
     1921 ctttatcaag tgcatatgca ctttttgacc taagcatata aacaccaagt cctttcgttc
     1981 cacctagcca ttctgcaaca gttgctgcca ttacagcata cgttgcagat attttaagtc
     2041 cagaaaaaaa tttatccatt gccataggaa atttaaggtg aatgaatgta ttaactttat
     2101 ttgagtccat agtcttaaat aaatttaaat aatctttatc aatattctca atgccatcta
     2161 caaaacttat taatatagga aaaaaacaag taagcattac catcaatact tttggcattg
     2221 ttccaaatcc aaaccatatc ataaagagtg gtgctataat tattgtaggt atcatctgtg
     2281 taaccaacat aataggataa agacattttt ttattattga tacaaaatcc atcaatattc
     2341 caattaccaa tgatattata attgcgacta taaaaccaag tattgcttct cctatagtaa
     2401 ccacagcatg catggataaa ttttgataat ctttaaaaaa cacattaatt atatctactg
     2461 gtgtgggtag tatatactga ggaacttcta aaactcctac catagattgc caaagaataa
     2521 gtataaacaa aaatgttgct attggatata tactatcttt gactttatct atgatttcta
     2581 agtttttcat ctatagtcag acctccagct ttttcatcta ctctagctac tgttatgact
     2641 ctttcacatc caagattaaa agggatttta tgtacctttt ttaataattc aaaaacttca
     2701 tcaaaatcac cttcaacagt agtgctcatt gctcctattt cacatttaag accacttttt
     2761 tcaatttcag caatacaagc atctactact ttatacattt cttcttcacc tttgtaaggt
     2821 cttagtggca ttactgctat gtctgcgatt accatttaaa tcactccttt aaaaataatt
     2881 aatacctact aggtatattt tgttttttat taaaaaattt tattactaaa aagatattat
     2941 atttaaataa aagaaaagtc ttatccaaaa taggataaga cttattttta tcttaactgg
     3001 tctccctaca ctggcattat ccaaacaggt tcaaagggtc gagataaaat ctcctctcag
     3061 cacattagct ccccgaaata tattatatct tttctacttg attatactac acttatcata
     3121 ttttaacaaa ttttttattt atattagata ttttatccgt tcaaaaatat ctaatataaa
     3181 ttgtacatta ttacaccaaa taaattatct taaaatggta atttatcaac atcttccaaa
     3241 actagattaa ctgaattttc tagtttttat tttgctattt caagcatatt tggatgtata
     3301 tcaattccat acaaatattg tgctaaattt ctgttttcac taatctaatt tgcaatcgtt
     3361 acagattaca gattagaaat agcttttaga ctctacttat ctttatctac aaaaaatcct
     3421 tcaataaatt cagtaataat attcagtaat ctaaaaataa acctctatct agatttttaa
     3481 gatacaagcc ttttaggaag tatcttttgt gataatataa aaattactcg tcattatcat
     3541 caaaatcctc atcaaccaac attttatata agaatcaaac tctataatat aattatttta
     3601 tccaatttat ttttgagaaa taaaatattt ttttaatttt taaatttttt aatatgcacc
     3661 ttgaaaaaag aaacgttttc ctatattatg gtaatagtag aataaaacta aataggcaaa
     3721 actagagaaa tttagtgacg caaagctata gggactaaga cttatgaaat gagttatgtc
     3781 agccagttgc caaagagata ttttcttttt gtttttatga atgtttttta gggggatata
     3841 tgatatatgt ttatggataa aatggataga tgtacacata tactaactgc atatatatgc
     3901 agttcacgcg attactgcaa ttttatagat acacaattaa atgattttat actagaatat
     3961 ggagagaatg tggtagaatc ttgtttgcat caagtgatgg tattggtaag cagatataat
     4021 taaagaaatt ttattttcta gaaaaccctt tttagtttta tctacgtttt ataattcatt
     4081 aaaaaactaa ttaaaataag cttcattata tatttttcat gaagtaaaca gttgagttta
     4141 tctcatatac tgtttacccc aattacataa ttctaataat atagattcca atgatttacc
     4201 acgtttagta gacaaatatt caacttttgg aggaatagtc ggataagaaa tcctcttaat
     4261 aagtccatct atttcaagat actttaattc ttgtgacagt atcttatgac ttatatgaac
     4321 aacttctttt tttaattgac tgtaacgtat tggctcttgt tttaaaatat gccataaaat
     4381 aattgccctc catttacttc ctatcacagt catggtaaaa ccaattgaac aattaaaatc
     4441 tttttgtctt tcttcaatat ttggaaattc ttcaaaatta ttatctatat ccatctcaat
     4501 cctctctttc aaaatagtaa gtatctaact aaaaagtgca ttcttgttta gcgtactaat
     4561 tcgtaatata atcttgattg tagtatagag ccttacataa tactactttt ttagatgtat
     4621 atgtttaaat tttaatatag acatatgtta ttttattaat tttatttaaa cagataataa
     4681 taaataataa tttatctcaa gttttctatg tagataaact aatctaatgt ttagaaagga
     4741 ttatataata tgaataaaaa atttcgtctt tatcaagttg actcattaca aaagaaaaat
     4801 ttacaggtaa tccggctggt gtaattacaa atgctgatgg cttatctgaa tcacaaatgc
     4861 aaaaaataac aagagaatta aataattcag aaactgcttt tattttcaat aacttggcta
     4921 aaaatcatca agaaaattat gatgtacata tcagattttt tacactaact aatgaggttc
     4981 ctatatgtgg tcatgctact attgctgccc attatgttag agcagtagaa aaccaattca
     5041 ataacaaaac agtatttcaa aaaactggtg ctggaatatt accagttgat attataaaaa
     5101 aagataatga ttatgaaata attatgacac aaggaaaaat tgaagtatct ccaccattac
     5161 aacaaaccta tcaaaaaaga ttacttaatg catttggtat tttaaaggaa gatattcgta
     5221 cagattgtcc tattgccata tcatctacag gacattcaaa agttatgatt ggaataaagg
     5281 aattatcaac attaaatagt ttatctccaa atttatatga attatcgaaa ataagtcaag
     5341 aaataaaatg taatggacat tatattttta cattacataa taaagaaact ctacaagttc
     5401 atggaagaat gtttgctcct gcaattggta tttcagaaga ccctgttaca ggaaatgcta
     5461 atggaccctt aggtgcgtac cttgtacatt ataaagtaat acctacaccc aacacagagt
     5521 ttatatttga agcagttcaa ggagaagcaa taaacgcaaa ggcaagatgt tagtaaaagt
     5581 aattatttta gataataagc cagttgaagt aaaaataatt ggaaaagctg taattgcatt
     5641 ctcaacaaac atagaaatct aattatagat tccttctaag taaatttttg aactttttaa
     5701 tactaattta ataacaaact aatttatata caatcaaatt aattttttta ttatttgtta
     5761 atatatattt atcattatat tatataataa tatagatagt tactatatct tataattaga
     5821 taaatcataa ttatatatta ttgttaaaac tcaattatat aatttttaat aatattattt
     5881 taggaggtaa aaatgataaa cgatattaaa aataaattca aaaattacaa accatatata
     5941 aatggataca agaatatgaa gagagcatct gttttaattc caatagtaga aataaataac
     6001 acacactata tattatttga agttcgctca aaaaatttga agcatcaacc tagcgaaatt
     6061 tcttttccag gtggtaaaat agaatcaggt gagactccat atgaagctgt tattagagaa
     6121 acttgtgagg aacttggaac ttttagtgac aatatagaag taatatcaag tcttgacctc
     6181 cttataacac cagtaaagtt tatcatacat ccatacttag gatatataaa aaatattaat
     6241 aatttaaata taaacaagga tgaagttgac catacttttt tagttcctat aaaatatctt
     6301 ttagagaatc ctccaaatac ttatagtaat actgtaaaag tttttccaga tgaaaatcta
     6361 ccatatggtt caattccaaa ccaaaaagat tataaatttg aaagtggaag ttatcctatt
     6421 ctgttttata aatataaaga ttatgtgatt tggggaatga cagcaaaaat actagaaaac
     6481 tttttaaatt ttttatcaga ataacactca taataaatta tcaaaacttt tacataagaa
     6541 atttataaaa gtttctaaat ttaacaattc taaaatatca ataactatat tttgtagcac
     6601 ttttttattt tattattgtt tgaatatttg atggttttca atgcaatttc aacataaata
     6661 ctatgtatta gtgtatactg ttgatgaatt aaagtatgat aatctattta ataaagtatg
     6721 aagtaaatga ttttaatgtg cctttatggt aaaattacta ataagtaata aaattaatta
     6781 aaatatatgg aggtgcttat aatgtttgct gtaggatttg ttgttataat tgcacttgcc
     6841 tttttcattt taagtgtact attatttaat ggaaaagcca gtatgctact agcaggttat
     6901 aactctatga gtaagaaaga aaaagcacga tacgataaaa aaaaattatg tagaagttct
     6961 agcattgtta cttttattgt ctctataatg ctatttatta tggcgttttt gggatataga
     7021 atagagagtg gtcaaatgga cgaaaaaaaa atggttccct tttctataat atttataata
     7081 gtcatacttt ccagtgtatt ttttaatatc ctctactcta acaaaaaatg taaaaaataa
     7141 atagttgtaa gttgagcagt taattataaa agattgactg ctttttcatg tatattgact
     7201 actcttatta aatattttaa catttaaaat ctatgtaaaa ataatgaata tagtacaaac
     7261 ttctaataat gattttacct ttgtggttac agtccactat atataattta atatgataac
     7321 agggaattac taatgacaac taggtaagct tttataaaat ataataaaac aaaaatagat
     7381 gtacaatgaa attatatctt atatcaaatt tatttttatt aatagcaaaa acttacatta
     7441 gcataacata tgacaaattc gcttaaaata aaaattttaa atataatttg ttctatagta
     7501 catctatttt gattagttta cataatttta ttattttgta caatattaat tatttactaa
     7561 tatcccattt aatgaaaaag ttctaggttc agtaattttg accataacta gcttaccaat
     7621 taaatcatca tttccacctt cgaaatttac gagtttattt tgtctagttc taccagcaaa
     7681 cttattttca ttatttttac ttcttccttc aactagaact tcaactattc tatctttata
     7741 tccatcattt atttctgcag aaatcctatt tacagcttct aacaccctat taaatctatc
     7801 atgctttata tcttctggta tttgattttc cattttagca gcaggagttc cctgtctttt
     7861 agaataaata aatgtaaaag ctgaatcata tctaactttt tcaaccacat ctaaagtgtc
     7921 caataaatct tcttctgttt ctcctgggaa accaatcatt aagtctgtag aaaaagcaat
     7981 acctggaact tcctttttag ctttttctat tatttctaaa taatattcct ttgtataatg
     8041 tctattcatt tttttcaaaa ggctactgct tccacattgt attggtagat gtaaaaactc
     8101 gcacacctta tcacaatctc taattgcgta tattacttca tcagatatat cttttggatg
     8161 agatgtcata aatcgaaccc tttcaattcc ttctatatca ttcacttttc ttaaaagttc
     8221 tgagaatgaa actggatttt caagtgtctt accataagaa tctacatttt gtccaagtaa
     8281 agtgatttct ttcgttccat ttgcaacaag ctcttttatc tcgttaatta tatcttcagg
     8341 agttctactt ctttctcttc ctcttgtgta tgggactata caatatgtgc agaaattatt
     8401 gcatccatac attatattta caaatgcttt taattcaaat tttctattac ttctaagacc
     8461 ttctattact tctccatcca catcccatac atccactaac attttgtcag aatttattga
     8521 ttctgtaagt aattgcggaa atttatatag attatgtgtt ccaaatataa ggtctacatg
     8581 tttatatttt tttcttaatt cctctacaac atgtggttgt tgcatcatac atccacatac
     8641 agctattttc atatctggat tttttccttt tagatgtttt aattgtccta agttaccata
     8701 cacttttagc tctgcatttt ctctaactgc acaggtgtta tatattatta aatcactttc
     8761 ttcaaccatc attgacattt ggtatcccat ctcttctaac attgaacata atttttctga
     8821 atcatgttca ttcatttgac acccgaaggt ttgagtaaac acgagttttc tctttccagt
     8881 tctgttgaag tattcttcat tttgtctgtg tatttgttct atatatttat cttgttcttg
     8941 tattttctct ataggtattg ttacttgttt tcttttgctc acgtctattc ctcctagtta
     9001 atttcttata ttacacataa ccttaattat atcaaataag aaaaatcttt taaagatatt
     9061 attatttttc tagttgtgtt ttaatagtaa atctaatgat ttatatatac tatatttcta
     9121 aaatataaaa tcatataatt attataataa taaaaagatg ttttaaatta aaacttaaaa
     9181 catctttcct taaataaaat atatgattta ctattaaata caattacatc tattcatttt
     9241 caattaaata acaatcattt aatattaaat attgatcttc ctcaaccgtt atatatcttc
     9301 ttcctgaaaa agtatcatta gtaactattc cctctgcatt acctaagcta tcatttctaa
     9361 cttcataata tgcatcagaa gaatttgaca ttataatata ttctccagca ggaatttcta
     9421 atccaatttt atacatacca tttccaaact taccattctt ggccttctgg gctttagcat
     9481 actttgctaa tgtcattgtt gaatcctcaa ttttaatata ttgaccattc tttaaggtga
     9541 tatatctagt cccactaaaa atatcatttg aaagtatact atcagcatta cctgtactat
     9601 cacttgtaac ttcataataa gaagcataac ttcctgagtt tgaagttatt aagtattcac
     9661 ctgctgagat atcttttcca accttataca ttccatcttt tatagtcttt gtagtaggtt
     9721 taacattttt tacaacccca gtattaatat ctccaattcc atttgttata tttaaacact
     9781 gttgtactat atttgaatct atatatccta tagaagttat cttcttagca ttttttaaaa
     9841 tgcttttatt gctaccatca ttaactaaaa ccattggacc atgttttgat aaagaagatc
     9901 ctacaagtgc atttgttaat tcataagctc cagctatata aaactctcta gcatccttat
     9961 aaaacttatt tattattgct ttattagttt caaaacgagt actcccccct agtctagtag
    10021 atttagtatt gtttactaaa gtggtactca ttgaagctgt tcctcctatt acataagact
    10081 ttaaaccact agttgaaaaa ggtatacttt gtccattagt aagaattatt ggtgctttat
    10141 ctcttgctgc tacagatgca atacttatag catctgcttc tcctttatat gcattggtta
    10201 gcataacagt attgactttt tttattgaat ttatttcttt tgcaacatta taacttgttt
    10261 ttattctgtc atttccctta attcttacaa ctttcatttt cttagatttt aatgaatttt
    10321 cgacagatgt actaatagaa tatgttcctc caattatata aacttttgat acatttttta
    10381 atcttgcaga tgtctcagtt ggtatagtat ttttttcagt taatagtata ggggcattta
    10441 aagctcctgc tagtccactt gcacttaatc catctgcaat agagttacta gtattaatta
    10501 atatagcagt tttatatgca tttttatctg ctattttacc tgctatttca tacttatttt
    10561 ttccttgtat tttttctaca gatgatattg catctactaa ttgaatattt gcaaatacaa
    10621 aagaaatact taaagtcaat gttaatattc ttttatagaa tttcataata aaattgtccc
    10681 cctcataata aatttttaat atataaatac atattcttaa attagttatg tacttaacaa
    10741 atttatgtaa aattgcaaat tatattaagc atattacaaa taaatattaa tacaattatt
    10801 catttagcta taattgtatt tacatttata ttaaaaatca actagatttg tataaatact
    10861 tataattata tttttattct taatattttt tttattatgt tattttaa
//
LOCUS       DAEMIK010000144        10842 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEMIK010000144 DAEMIK010000000
VERSION     DAEMIK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.146"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            298..1146
                     /locus_tag="KPK87_003128"
     CDS             298..1146
                     /locus_tag="KPK87_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG2539251.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1210..3132
                     /locus_tag="KPK87_003129"
     CDS             1210..3132
                     /locus_tag="KPK87_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2539252.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLSMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3214..4077
                     /gene="aroE"
                     /locus_tag="KPK87_003130"
     CDS             3214..4077
                     /gene="aroE"
                     /locus_tag="KPK87_003130"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG2539253.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGIGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVSECIVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4277..5494
                     /locus_tag="KPK87_003131"
     CDS             4277..5494
                     /locus_tag="KPK87_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2539254.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5730..7595
                     /locus_tag="KPK87_003132"
     CDS             5730..7595
                     /locus_tag="KPK87_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG2539255.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7773..9137)
                     /locus_tag="KPK87_003133"
     CDS             complement(7773..9137)
                     /locus_tag="KPK87_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2539256.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9316..10659)
                     /locus_tag="KPK87_003134"
     CDS             complement(9316..10659)
                     /locus_tag="KPK87_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG2539257.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10732..10833)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         4034 a         1367 c         1844 g         3597 t
ORIGIN      
        1 aaaatttatc gttttttgtc tgaattataa gataaatata tttatacgac ctaatagaaa
       61 aatacaagaa ttatcttata aataaaatct ataaacaaaa tctatgaatt aataggaaat
      121 tatcaatttg ttattagtaa agtctcgtga tacaataaat ttataaaatc tagataaata
      181 gaaatcaaaa aatactttta tttttaaggt aaaataatac tactagtagg ataaataaaa
      241 atataaaatc aaaaatacta ttaattataa agaaaataaa gggggtaaat ttaagctatg
      301 aataatcaaa aaaatcttaa tattccaatt acaattagtt cttatacatt gggaacagaa
      361 gtttcattcc gtgatagagt tcgtattgca aaagaagctg ggtttgatgg tattggattg
      421 cgtgctgaaa attatattga tgcaaaaaat gcaggtgtta cagatgaaga aatgattgca
      481 attcttaatg aatatgatat gaaagttaca gaagtagaat atataactca atggggaaga
      541 gaagaagata gaacggaagc tcaaagagaa aaagagcaaa atgtttatca tatggctcac
      601 ttatttaatg ttaaacatat aaattgtgga ttattagaga tgataccaga agaacaaatt
      661 ataactgcat taggagaatt atgtgacaga gcaggtgaac ttattatagg tcttgagttt
      721 atgccatata gtggtgtacc agatttggct actgcatggc gtgttgtgaa aggatgtaat
      781 cgtgaaaatg caatgcttat attagatact tggcactggg ctagagcaaa gcagacagca
      841 gaggaccttg cacctgttcc agcagataag atagtatcaa ttcaagtgtg tgatgtttta
      901 gaaactcctt atgaaaaact tagggatgaa tcactacatg accgtttggc tccaggtgaa
      961 ggatatggaa acacagagga atttgtgaaa ataataaaag aacacaatat atcaccaaga
     1021 gtcatagggg cagaagtaat atctgattct ctaatatcta aagggttatc cactgctgct
     1081 gaaactgtat ttaatgcaac taaaaaagtt ttggataaag catggtctga agtatctcca
     1141 aaataaactt atataatata aaaatataca gagtattata atataaaact tgaaaggaag
     1201 gaaaacctta tgaagattag ttctatgttt acacctataa gaattggttc aatgacagtt
     1261 ccaaatcgtt ttgtagttcc ccctatggga aacaattttg caaacacaga tggtacttta
     1321 agtgaaactt ctaaggctta ttatcttgag cgtgctttag gtggttttgg attaataact
     1381 atagaatcta gtgttgtaga caaaaaagca aaaggaggtc ctcgtaagcc ttgtttatat
     1441 gatgatagca caatagatag ttttaaaaat gtaatagatg cttgtcatga tgcaggttcg
     1501 aaggtatcta tacaattaca acatgctggt tctgaaggaa atgaaaaagt agcaggacat
     1561 ccattaaaag ctgcttctgc aatacctgca tcaaatggtc gtaatacacc actagcaata
     1621 acaacagaag aaatatatga acttatagaa tcttatggag atgctgcatt acgtgcacaa
     1681 aaagctggtg cagatgctgt tgaagttcac tgcgctcatg gatatctggt tagtagtttt
     1741 atatcacaaa gaacaaataa gcgtgtagat gagtttggtg gatgttttga aaatagaatg
     1801 agattacctc gtcttatcat tgaaaatata cgtaaaaaag taggaaattc tctagcaata
     1861 ttatgtcgca taaacagtac tgatgatgtt ccaggaggaa ttgatgtaca cgatagttca
     1921 gtaatagcag catatctaga agattgtgga atagatggac ttcatgtttc acgtagtata
     1981 catatacatg atgaatatat gtgggctcct acgactttac atgctggatt cagtgcagaa
     2041 ttagttactg aaataaaaaa agctgttagt ataccagtaa taacagttgg acgttataca
     2101 gagccacagt ttgctgagtt aatggtaaga caaggaagat gtgatttagt agcattcggt
     2161 agacaaagct tggctgaccc agaaatgcct aataaagcta aaaatggaaa actagacgaa
     2221 atgataccat gtattgcatg cttacaagga tgtgtaccaa atatgttcca aggcaaacct
     2281 atagcctgtc ttgcaaatcc tatattagga catgaagcag agcttaagcc agctgaaatt
     2341 agcaaagaag ttcttgttgt tggtggaggt gttggaggta tgcttgcagc atgggtatgt
     2401 gctaaaagag gacataatgt aactttagtt gaaaaatctg aagttcttgg tgggcaaatg
     2461 cgtttagctg catatcctcc aggaaaagga gatattacta acttagttag aagttatatt
     2521 tcaaaatgta atcaatatgg tgtaaaaata tgtactaata cagaagctac agtagaactt
     2581 ataaaggaga aatcacctga tgtagttatt atagctactg gagcaactcc acttgtactt
     2641 cctataccag gaatcaatga ttcaggatta attcatgctg tagaccttct tgatggtaaa
     2701 aaatcttgtg ggaaaaaagt acttgttgtt ggaggtggaa tggttggatg tgaagtggca
     2761 gccttccttg gagaacaaga acatgaggtt actgttattg agcttagaga agaagttggt
     2821 gcagatgtaa tttcagaaca ccgtaaattc cttatgaatg attttgaaaa ttataatata
     2881 cataccatta ctggtgcaaa agtttctaaa ttttatgatg atggtgtcga ctatttactt
     2941 acagatggca cagagcatga tttaaaaggt tttgattcag tagtactttc tatgggttct
     3001 cgtaattacg acccattaag tgaagttata aaagaagttg taaaagaaac ttatattgtt
     3061 ggtgatgcag taaaagctcg tagggcatta gatgcaacaa aagaagcttt tgaagtagca
     3121 ttaaatatat aaataataaa taaatttttc tttgtaaata actgaatagg ttatttgcaa
     3181 agaaaatgat aatattggga ggagattata gttatggaaa tttcaggtag aacaggatta
     3241 tttgcattaa taggtacacc ggttggtcac tctaaatcac cagtgatgta taattatagt
     3301 tttaagaaat tagatttaga ttatagatat ttagcctttg atatcacagt agataaggta
     3361 aaagaggcac ttttagcaat aaaaactttt aatataaaag gagccaatgt tactatgcca
     3421 tgtaaatctg ctgttacaga atatatggat gaactttctc cagcagctcg tataattgga
     3481 gcatgtaata caattgtaaa tgacaatgga aagttagttg gtcatataac agatggaata
     3541 ggctatgttc gtaatttaaa agaaaatggg gtagaagtta aaggtaaaaa gattacaatt
     3601 atgggtgctg gtggtgcagc aactgctata caagtacaat gtgctttaga tggagcaaga
     3661 gaaatatcta tctttaatcc taaggatgat ttttataaaa gagcagagca aacagttgag
     3721 aatataaaaa aagatgtatc agaatgtatt gttaatctat atgatttaga agatacaaac
     3781 aaactttatg aggagataca atcaagtgat atattgacaa atgcaactct tattggtatg
     3841 aagccctatg acaatgaaac aaatataaaa gatactagtg ttttaagaaa agatttagta
     3901 gtaacagatg tcgtttataa tcctaagaaa acaaaaatga ttgaagatgc tgaagctaat
     3961 ggatgtaagg ctattggtgg attaggaatg ttattatacc aaggagcaga agcttttaat
     4021 ctatatactg gattagaaat gcctgtagaa gaagttaatg aattatgctt taaatagata
     4081 agagtaatat cttaaacaat gaaaaaacat acgtatttca atatttttaa taataaatta
     4141 caaattgtca tagtgggttt tgcctcatag tccagttaaa tttgaagctt taactgacta
     4201 tactttaaat ccattatggg gatgagttta ttgtacacaa aaatgttaat aaattaacaa
     4261 gaatgggaga aaaattatga acaataaaaa atattatcct actgccatag cgttgtattt
     4321 ttcatatttt ttacttggta ttggaatttc aattttagga caatacaaac cagaattttc
     4381 atcaatgtgg ggggctaaaa cattatcaga tggaacattg gatgtaagta ttgttttagc
     4441 tgttattgct gcactaggat taggtagact tatatcttat ccatttgcag gacctatttc
     4501 agataagtat ggaagaaaag taagtggtct tataggaaac tttctacatg caattttctt
     4561 tgttggaatt gtattttcac caaattttta tatagcatat gtatttgcaa taattggagg
     4621 ggcagcaaat tcattcttag atacctgtgt aactccttct tgtatggaga tatttgctag
     4681 tttaggaaca atagcaaata tgtttactaa atttacaata gctttagcac aatttttgct
     4741 tccttttata attggatttg ttgcagcaaa ttcaatatca tttaaagtta tttttatagt
     4801 aacagcgatt cttattgtag tagatgccat tttgatagct gtgttaccat ttccaccagc
     4861 taataatgtt attgataaca aaggcaaaac agttaaatca gagaagatga aatttacacc
     4921 aacaagtata gctcttgttt gtattggatt tacatgtaca tctacatttg tattatggtt
     4981 aaattgtaat caggaattgg gtaaattgta tgggatggct gaccctacaa aaattcaatc
     5041 cttttactca atgggagtaa tatgtgcagt attaataact tctttactta ttaaaaaata
     5101 tattaaacca ataagaatat tagttatata tcctataata gctttactta tgctattagt
     5161 tgtatatttt gttcaaactc caactatttg tatgttaggg ggatttgtaa taggttattc
     5221 agcagcagga ggtgttcttc agttaacaac gtcaactgca aatgaaatgt ttccaactaa
     5281 taaaggaaaa ataacatcta tagttatgat tgcatctagt atagcaaact atgttatttt
     5341 aaatatagca ggtattatta caaaatcagg gggagttaat ggaccaaaat atgttgtatt
     5401 gtttaatgtg gcaataacat ttgttggaat tttacttgca ttgtttgtaa acatgagata
     5461 tgaaaaagaa aaagtgtatg attatgatgt ctaatattag tataatatat gcatagagaa
     5521 gtctaaatct aggatagatt tttattaaaa gtaattaaaa ttaataagga tattatttac
     5581 agttataaga tagggtattt acataattaa taataaggat attactagaa tttcaatttc
     5641 tagtaatatt tttttgcgta taaatgtaaa cttgacgaaa taagtagtat aatcaatata
     5701 aattacatca tgtaaaggag gataatttgg tggagaatgt ttttaagcga ttgcaggaat
     5761 ttaatggtta tgatggatac aaagaatcat ttgaaatgaa ttacctatgt atatatgaaa
     5821 gtataccgtt aagagaacaa gtagagcttg caaataatct agtagatgaa attttaaata
     5881 tgtataaaag tgaatctaat gaaatttatt tattggagga tagtaattct aagtctttaa
     5941 tttgttattt cgaaattttt atgaaaaaga taaatacttt ggtaaaagaa atgattattg
     6001 atgagaaatg gttatataaa ttaactaaag aattaatata taagagtaaa aaagttgagt
     6061 atgtaaaact agggttagtt ttaagtgaga aatatctaaa tgtagaaaac ttaagagaag
     6121 ttgtagatac attttctaaa tctggagaat acgtatttta tctgagtaat actattaaaa
     6181 aacttgaatt ttacaataca tatttattta atttatctaa aaaggctaca ggttcaataa
     6241 aagtatttgc tattgttaat atggaaaatt tagattctaa aataaattcc tatctaattg
     6301 aagatggtta taaggataca aagtacgaac gattattgat gaactatatt atttcaattg
     6361 tagatttgaa tgaatatctt gaaaaacgag atttagataa agaaaagata aataatttag
     6421 cacgtcttat atgcaattac ttactaagtg tagaatttaa atatataggt aataaattag
     6481 aattagtgaa tcgattttta cctactgtag taaattatgg gactaacttt gaaagtttat
     6541 attcaatttt cctaatagct ataaacgtct taaaagatga aaatatagag tgcaataaga
     6601 ttgaatttga aaaagaaata aatgatatct tgctcagtga aaaatggaaa aatatatatt
     6661 ttgaagcatt aagagatgct agtggaaaaa cagaagatat tataaaaatg tcagaaattt
     6721 atgatgtgaa cttatctttt gatgacttgt tgccatatct aaatagagat attagagatt
     6781 ttgaggtata ttggcatata tctaaaaagg gaacgacaag ttctagatta aaattattaa
     6841 atttttttga agagacgttt aaaatagatg atttaattgg aaaaatgaaa gatatagaaa
     6901 aagataaatt gactcaagaa tattatgatg atatgttatt ttttattgtg cttaaaggaa
     6961 gtaaatcact atatcctgaa ggaaagaata tatctttaaa aggaatattt ggaaatataa
     7021 atgaagtaag aaaagaatcc ataaatatct taaaaagata cagagagaaa ctatctcttg
     7081 aagaattaaa aattgttaaa gaagcgtatg aaaaagaaaa aaatgttata ttgaaggatg
     7141 aattaagaag agtattatat gaaagtaata atttaaaaaa agaatttgta aatattgaaa
     7201 aaataaaagt agatgaacat ggtaaggata tctatttgac ttcaatagct gttgctggtt
     7261 caaggtttag aaatagagaa tatcttgaaa aagaattgga gaaaagtaag atatactatt
     7321 taactagaga gaaagataat ctatatgatg aaaaagctat taaaatagtt ggtgaaactg
     7381 gatatgtgat aggttatgtc ccaagaaaag aaaactatat tttgagcaat ttattagatg
     7441 gaggaaaact gctgtattgt agagtaactg aatataattt atatgaagat tgtatctacg
     7501 caaatgtgta cttgagttat aaagatgtaa ttgaaacagt tgaaaactct ttaaagatgg
     7561 ttttagataa gagtagaata aaacttataa actaagacgt ttatatattg cttagatgat
     7621 gtaatcttaa ctgattggtt attttttctg gaattatggt gtattttatg agcaatgcta
     7681 atgaatataa aataaaaaaa caattgtttt gtatatatga tgtatataaa tatatttata
     7741 tattcatatt tacaaaacaa ttgaaattta tgttatttta taattttaaa tttagatgga
     7801 attttaactt ctgctttatc ttttatttta gaagcttgtt ttttaacttt atttaataat
     7861 tcagattcat ctacaactaa tacttttctt tccttcatca accatttacc atcacaaata
     7921 gtagagtcta cattttcact agtcatagaa taaactatat ttccaattgg attatgcatt
     7981 ggtaaacaat gaattgtatc tggatttaga ataatcatat cagctttttt tcctacttca
     8041 agactaccaa tttctttttc tagtaaagca cattttgctc catttatagt agccatttct
     8101 aagacttctt cagctggtac gacagttggg tttaaagttc ttcctttatg tattagtgat
     8161 gtaagataca tgtccctcat aaggtccatt ctattatttg aaggagctcc atcagtacca
     8221 attgaaacag gtattccttt ttctaacatt tcaggaattg atgcaaaccc tagaaccact
     8281 ttcatagctg aacctgggtc atgagaaact tttacatcat gtaatctaaa taaatcaatt
     8341 tccttatgag taagccaaac tgtatgagca gctaaaagat ttggtcctaa tacacctagt
     8401 ttatcaaggt attcaactgt tccaactcca ttgttttgct ttacaaatga aatttcatca
     8461 gctatttcag caatatgcat atgtatacct gtattgtatt tatcagctag ttctttagtt
     8521 ctaacaagta attcttcagt tgtattaaaa atagttctta atgcaaacca tattttaatt
     8581 ctctcattag atgtattatt aaacttatgg aataagtcta attgagtgtt gatttcctca
     8641 tcagtagttt tttgccatgc ctcaggaaga ccatctcctg tatccataac tgatttagct
     8701 aaacaggctc taagtccagt tttatcaaca gcttcaacca tagcatctac atattgacca
     8761 ccagattcta aaaatgtagt gacaccagat tttatcaatt caacacaaca tgcagtagat
     8821 gaaatcaagg aatcttcata attgaagcta ctttcgtatg gccaaattct ttctctaagc
     8881 caagttaaaa gtacaacatc atctgcaaga ccacgtccta attgttgaga taagtgaaca
     8941 tgagtattga ctagccctgg cattagtatt tttcctttag catcatatat ctctgcatct
     9001 ttatctacaa tattaatatc aaacttacca acctctttta taatattatt ttcaataagt
     9061 atgcttccat tttcatatac ctctctatct gcattcatag taattagata tgcatttttt
     9121 ataagtaatt gtgacataaa caccactcct tttctatata tttttatatc tatatattat
     9181 gataataaat tttctataca aagttaatag caaaatcaat ttacatataa agtgattttg
     9241 ctatttataa atttaaaata aataagattt tattctatat aatttaaaat attgacctag
     9301 aactatttaa aaatattaac ttagaactaa gtttacaact gcaagtaaac ctataatcca
     9361 aagtgtaata ctgattttat cagtttcttt agctactaca tgcattatta tatatgaaat
     9421 aaatccaaag cataaaccaa tacttatact ataagttaaa ggcatcaata aaactgtaac
     9481 aaaagcaggt acagctattt tgctatcagc aaaatcaatc tgttttatgc tttcaaacat
     9541 aaacacacca accattacaa gtgcaggagc tgcagcgtaa gtaggaacta taccaagtaa
     9601 tggtgtaaat ataagtgtag ctaagaataa tgctgctgtt actattgaag caagtcctgt
     9661 tcttgcacct gctgcaatac cagaagcaga ttctccaaat gttgtaacag tactagttcc
     9721 taagaatgag cctattaaag tagaagaaac atctgcatat aacattcttc ctaaatcttt
     9781 tatttcacca ttctcatctt caagacccat ttgtttacaa caagtaataa gaaatgctaa
     9841 tgaatcaaac atgtctataa acatgaaaga gaatataggt ccaattaatg agaatttcaa
     9901 tgcacctaca atatctaatt taaaagcaac tggtgctata ctaggaggtg ttgagattat
     9961 tgctgttggc aactccacaa atccaaatat aacagatatt attgttgcaa ctactatact
    10021 tatcaatatt ccacctttaa cttttttata ttctagtatt gccattaaga taagagcaaa
    10081 aagggctatt aaaactgttg gtgtaaaatc tgcaagtgaa actatagtat ctggattagc
    10141 tattataaca cccatatttt ttaaaccaac aaaagcaagg aaaagaccta tacctgctga
    10201 agatgctact gccaattctc taggaattgc gtctgcaatt gtctttcgaa gacctgttac
    10261 tgcgagtgta aagaagacac atccagatag aaaaactacc ccaagacctg tttgccatgg
    10321 gattcctgca cctataacca aagagtatgt gaaaaatgca tttaatccca taccaggagc
    10381 tagagcaaat ggtgcatttc ctaaaatcgc aaatataagt gtaccaatgg ctgtagctag
    10441 tatggtaact gttataaggg cggctttatc cattccagct tcagataata ttgctggatt
    10501 tacgaaaact atgtatgcca ttgttaagaa tgtagtaact ccacctatta cttctgtttt
    10561 aaggctagag ccacgttcgc taaatttaaa cattttgtct aaaaagctta atgatgaatt
    10621 atttccacct ggttgtacat tgttagtgtt tggtaccatt tttacctcca aaaaacttta
    10681 agatataaac aaaaaaatat atattcatat agatagaata gaacatatat tcagagttga
    10741 cattctattc atagaaaacc catttaaggt gagtttgtag aaactattag accgtattac
    10801 taatattata tgaacatttt ctatttgttt ttctgttgta tt
//
LOCUS       DAEMIK010000145        10443 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEMIK010000145 DAEMIK010000000
VERSION     DAEMIK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.147"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..721
                     /locus_tag="KPK87_003135"
     CDS             104..721
                     /locus_tag="KPK87_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG2539258.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(878..1330)
                     /locus_tag="KPK87_003136"
     CDS             complement(878..1330)
                     /locus_tag="KPK87_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG2539259.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1778..3136
                     /locus_tag="KPK87_003137"
     CDS             1778..3136
                     /locus_tag="KPK87_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2539260.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3409..3789
                     /locus_tag="KPK87_003138"
     CDS             3409..3789
                     /locus_tag="KPK87_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2539261.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3914..4114
                     /locus_tag="KPK87_003139"
     CDS             3914..4114
                     /locus_tag="KPK87_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539262.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4310..5038
                     /locus_tag="KPK87_003140"
     CDS             4310..5038
                     /locus_tag="KPK87_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2539263.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5060..5977
                     /locus_tag="KPK87_003141"
     CDS             5060..5977
                     /locus_tag="KPK87_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG2539264.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6119..7303
                     /locus_tag="KPK87_003142"
     CDS             6119..7303
                     /locus_tag="KPK87_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2539265.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7410..7598
                     /locus_tag="KPK87_003143"
     CDS             7410..7598
                     /locus_tag="KPK87_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG2539266.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7747..8397
                     /locus_tag="KPK87_003144"
     CDS             7747..8397
                     /locus_tag="KPK87_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2539267.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            8500..9438
                     /locus_tag="KPK87_003145"
     CDS             8500..9438
                     /locus_tag="KPK87_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2539268.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9464..10315
                     /locus_tag="KPK87_003146"
     CDS             9464..10315
                     /locus_tag="KPK87_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2539269.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         4239 a          997 c         1847 g         3360 t
ORIGIN      
        1 aaaaaaagat attgggtatt taaagtaata ctttatttaa ttttaagtaa atcgggtatc
       61 tacatatata gatactaatt tcaaagaata ggagggtgtt tatatggaaa tattcgcaat
      121 aggacattca aattatccat atgataagct tattaatatg ataaaaaaat atgacataaa
      181 ttgtgtagtt gacattagag aaactccata ttctaaatat aatattcaat acaataaaga
      241 ggcatttaac gaatctctta gaaattccgg atttttatat atatacatgg gaaaagaatt
      301 tggagctaaa agaactaata aagatgtata tacgcaagaa ggttatgcag attttgagaa
      361 agtagcaaaa gaagatattt ttttaaacgg aatagaaaga ttaaaaaagg gttgtcaaat
      421 gggttatagg atagttttat taggagctat gcaagaacct ataagatgtc atagaagtat
      481 cttggtggga aaagtattaa ataaagaagg ttttgatgta aaatatataa tgcatgaagg
      541 aaatttggca tatcaggaag atatagagga atcattattg gataagtatt ttagtgatag
      601 aaagcaactg agtattgaca atttattagg gagtgcatta acgagagaag aaatgataca
      661 agaaggttat aagctagcta ataaagaaat aggatataga actgagaaac taggtaaata
      721 aaaatataca agtagataaa atacagtact tatcttaagt aaaataatat tatgaattta
      781 taatagctag gcatgatgtg ccaattaaaa atattctagt tatttaataa tatcattaaa
      841 tttagggtat tttttaatag cacatcatgt tgcagtttta aaaaataaaa tctctaatat
      901 tgccataatc aactgttcta ttaagagtag gatgactaga tataaatcta actgtaattt
      961 ttgaatttaa caaacaagga tttgaaatta aaaatactct atctccattg acaatagaag
     1021 taatagctac tggcaaaata tctacaaatg tatcatatct gtttttagca atttgataag
     1081 gataatttcc tacaaaaaca aatagattta aatcatatat attatcagta aaccaagccc
     1141 attgaccata aactttgtcg atattcattg tgcctaaacc aagtgtatca aagtatgttg
     1201 ctatagttaa ataaatattt tccatgtttt ctaaatatga aacagtatat cttctgaaac
     1261 gtatgggtgc tgtggaagaa ataggataaa ccatatttac atttagatta tttgcattta
     1321 aattaaccaa agtatcacat cctaaaatta gtctataaaa tacaatattt aaatggatgc
     1381 gattttgtta catctaaagt gtaatatttt atatattgaa aaagtggtaa aagtttggat
     1441 gattaaataa tattataaaa aatatataaa atgtacttat tgttattgct acacgatagt
     1501 gatacaatat taacgaaata tagctacttt taataataac ggaaaatatt tatattaaat
     1561 taataaatat tttaaatgtg gtatccataa aaaataataa acataagata attataaaga
     1621 tatactgtgt tcaaatgata ttaaacaaga tttatattat ttgaaagcag tatattataa
     1681 tgcgaacaat acaattctaa actttgcatt gtcaatttac agttcgataa actaattaaa
     1741 aattaaattt atataaagag ggaaaagagg aatagggatg tttataagga aaaatgttat
     1801 aaagttagca attcctataa tgatagagca gacgtttgtc atgcttttag gagtatttaa
     1861 tacaatgatg gcaggtcata ttggggaaga agctgtatct gcagttggaa tggttgattc
     1921 aataaataat atatttatat ctgtatttgc tgcactatct gttggagcta ctgttgttgt
     1981 agctcaacaa ataggaaaaa aagatagtga aaaggtgaat gaaactatta agcaggctct
     2041 agtgtctgga gtaatattat cctttacaat aacaatattg atgtgggttt ttagaactgg
     2101 tgtaattaat ttattttatg gttccgcaga agaattggta aaatcaaatg caaaattata
     2161 catagagttt acattattta cgtatccatt tattgcgaca caacaaatag caaatggtat
     2221 tttaagaggg tgtggagatg taaagaaacc tatgtatgtg actatgttta tgaatatagt
     2281 aaatgtaaca ttaggatata tacttatata tggtattgat ttaaatcctc taggaataaa
     2341 tttgcaaaca ccatcctatg gcataacagg tgcagctata tctattgcta tagctagaat
     2401 aattggatgt atagtaatta taagagcttt atttaaagga aacgaattta tatctatggg
     2461 taaaatattt ccatttaaga ttgatgttga aactcaaaag tctatattta atataggaat
     2521 accagctgga gttgaacaac tactttttaa tgcaggtaaa ttgattgttc aagtatttat
     2581 agttactatg ggaacatcag caattgcatc caatgcaata ggaatgtcaa ttgcatccat
     2641 aataaatgta attgggaatg ctttagcttt gtctgctaca accttggtgg gacagtatat
     2701 aggacgtgga gatattaaag gagctaagag tactttatta tatttaacta aatttgcaac
     2761 aatttgttta tttatagtag gagctatatt tataccaatt gcaccctgga tttcaagtat
     2821 gtatactcaa aatgctagtg taattgacat ctcaacacaa ttaattcgaa gtgattgttt
     2881 agctatggta atatggccta tatcctttgt attatcagca gggctaaagg gagccggtga
     2941 tactagatat acaatgatga ctgcagtaat aggtatgtgg atttttagaa tttttacagg
     3001 ttacgtattg ggggttgtat tagaatttgg agtgttagga atttggatag gtatgtatac
     3061 tgattggctt gttagaggga ctttatattg tttaagatta agaggaacaa agtggctaaa
     3121 acacaaagtt gcatagaata tatgaaatca cttttagatg gataactcta tttagaagtg
     3181 attttttatg tagttattat cataattata agtatgatat ataaggtgat taaattacat
     3241 aaataaaggc tataatagta tggatattat aaattatata ttgagcagaa ttacataatt
     3301 tggcgataaa tattagaaat atatatctat agaaaataat atatatgggt atattattaa
     3361 atataaaaaa tattaggtat ttatttaata ataaaggtgg gtattgttat gataaataaa
     3421 ataggaaaaa taactctcta tgtaaataat caagaggatg caaagttatt ttggacagaa
     3481 aaattgaact ttgtagtaac ttatgaaaag gaaatgggtc caaatatgaa atgggtggaa
     3541 gtagcaccaa gtaaaaatga atttacaaca tttgtgttgt atgataaaaa tctaatgatg
     3601 aagcaaaaca aagagataaa tgtatctcat ccatcaataa ttttaagtac aagagatatt
     3661 aatggaactt atgaacaaat ggcaaataac gaggttgagg ttggggaatt aatggagatg
     3721 ccatatggaa ggatgttttc ttttaaagac caagatggaa atgattattt ggtcagagag
     3781 gataaataat tttacaaagt attaaattta ctcaatatct gatataatta gagacaataa
     3841 ttattataaa ctagtctagg attagaatta tataattaaa aattaagtgt aaattttgag
     3901 tagaggtgat aaaatgttta agaaaatggc agtactaaaa gatatagcaa ctaaaatagg
     3961 tcgtaaaaaa gcgtatgaac tattagaaat ggttgaaggt aatgatgcct ttgtagctga
     4021 ggtaaagata aaaaagaatg gaatagaatc taaaaaagaa gaaattatgt taaaagataa
     4081 tcaaaaaata atattagagt atatagaagg ttaaatttta aatacatata agttgatata
     4141 ctaaatttct gagatgagtt tacataaaac tcatcttttt tacttttcaa taactttgtt
     4201 ttattattta atatacattt tatctcctta gagttgctga tatataaaaa ttactgatat
     4261 aataaaagaa tggatattaa tgtaatattt tatagaaaag aggggtatta tgcttgaaat
     4321 aaaaaatctt tcctttaatg ttgagtctaa caatgaggaa ctaggaataa taaatgatgt
     4381 tagcctttca tttgagaggg gaaaacttat tgttatcaca ggtccaaatg gtggaggaaa
     4441 atctacaatt gctaagctta ttatgggaat tgaaaaagct acgagtggtc agataatttt
     4501 agatggagaa gatataacaa atcttagtat tacagaaaga gcaaaaaaag gaataggata
     4561 cgcatttcaa caaccaccaa gaataaaagg tatgacagtt gaaaatttac ttacacttgc
     4621 tcatggaaaa ccattgtcta ctgatgtatg ttgccaatac cttacagatg ttggattgtg
     4681 ttcaaaagac tatttaaatc gtgaagttga taactctctt tctggagggg aaatgaaaag
     4741 aattgagata gcaactcttt ttgctagaga tttaaaggtt tctatatttg atgaacctga
     4801 agcagggata gacctttgga gctttggaaa actcaatgaa agttttaaga aaatacatga
     4861 agagtctaat cagactataa tcataatttc tcaccaagaa agaatattag agttagctga
     4921 tgaaataatt gttctacaag atggttcagt aaaaagccat ggaacaaaag aggctatatt
     4981 acctgaaatc atgtgtcaag ttaattcaag ttgtgaatta atgaaagaca tgaattaatt
     5041 agaaaggaag gcaaattaaa tgaacaaaat agatttaaat cttttagaaa aagttgctga
     5101 acttcgtgga actccattag gagcatacaa tatacgtaaa aatgggcaag gagtagagag
     5161 acattcaact gaaaatgtaa ctataattcc taaaactgat aaacctggaa tagatattat
     5221 agtaaagcca aatacaaaag gagaaaatgt acatatacca gtaatagtaa cagaggctgg
     5281 tgtacaggat atggtatata atgattttga aattggagaa aatgcagatg ttgttataat
     5341 tgctgggtgt ggtatacata actgtggttg tgatgatagt gaacataatg gtatacatag
     5401 atttttctta cgtaaaggag ctcgcctaaa atatgtagaa aaacattatg gtgaaggtga
     5461 tggagaagga aagagaattt taaatccagt tactattgtc aatatggatg aggattcata
     5521 ttgtgaaatg gaaacaattc aaattaaggg tgtagattca actaaaagag aaatggatgc
     5581 aaacctaggt aaaggagcta aaatagttgt atttgagaaa ttattaactc atggtaatca
     5641 agttgcagaa tctaatatga aggttatctt agagggtgaa aactcttctg ctcaagtagt
     5701 ttctcgtaca gttgggcaag ataattctaa gcaaaagttt aatccttgtg ttattgggga
     5761 ttccctttgt tctgcacatg ttcaatgtga ttcaataatt atgggaaatt ctcaaattag
     5821 tgctatacct gaaattgctg caaatcatcc agatgctctt ttagtacatg aagcagctat
     5881 tggaaagatt gcaggagacc aaatactaaa gatgatgaca ttaggtctta ctgaagaaga
     5941 agccgagcaa caaatactta attgcttctt aaagtaaatt aaagatttta tttaatttaa
     6001 tataattaat atagtataat atagtgaata agtctaaaat atttttattt ttaggcttat
     6061 tttctttatg taagaaaagc tattagagaa attatattgt aattaggatg tggaataaat
     6121 gaaaaattta cttataagaa atgggacaat aatatctcca acagatggta tcaaatttga
     6181 aaaaagagat atcttggttt tacatgggaa aatatatgct attggattga accttaaaga
     6241 gaaaataaga aaagaaaaat tacttacaaa taaagagttc gagataataa atgccgataa
     6301 tatgtatatt tccccaggat ttatagatgt tcacacccat tgttataaga gaaaattacc
     6361 tacaggtatg gattctgatt caattggaat tgaaaaaggt tctacagtta tatttgatgc
     6421 aggtacagct ggtccttcaa actactatga ttttaaaaaa aagtatatgg atgagtgtaa
     6481 aacagaagta tattcttttt taaatgttac aaatgaggga ctaaatatat tgaatgaatc
     6541 aacttcttta gatgttataa attttgacaa ggttgagtca gttgtagaaa aaaatataga
     6601 aaagataaag ggtataaaag taaaagcgag taaatttcaa tctaatgaaa gtggtataga
     6661 tataattaag aaatcaaagc aagttgctaa aaaattaaat ttacccttag tggtatatat
     6721 aggagagtat ccatcatata tagatgaagt aatgaatatt ttgggaaaag gagatgtggt
     6781 tacaccagta tatggaaata gtacaaatgg aatccttaaa gaatctggca cattaagaaa
     6841 atcagtaata aatgcaaaga atagaggagt attgttcgat gtatgtcatg gagtggacca
     6901 atttgatttt aaagttttta aaaaagcaat aaagcaaggt ttagagcctg acctaataag
     6961 tactgatatg catataaaaa acatgaaaga cgtaaactat tctcttttaa atgttataaa
     7021 taaaattatg gaacttggga tatccctaga aaagtgtata gaaaaagtaa ctgaataccc
     7081 aacaaagatt ttcgatttac atggaaataa aggcagaata aagattggtg gagaaggtga
     7141 ttttacaata ttcacaatgg aggagtgtaa tgatattata aaagactaca ataataatga
     7201 acttgtttta aataaaaaat taagattgca atatactgta aaatcatgga tgaaaagcag
     7261 tgaagtttat agacatggat acgaaaatac tataataaac taaacaatag tgataaaata
     7321 ataaatatat gtttaaattg gtatatattg gataaatatc taaggaaaca acaattaaaa
     7381 aataataatt ttaggggagg tattttagta tgaaagcatt tgttgataag gatgtatgtg
     7441 tagcttgtgg agcatgtata ggaacatgtc cagacgtttt tagtatgagc gatgaagggc
     7501 attcagtagc aattgaagga gaaataccag cagatttaca agatttatgt aaagaagccg
     7561 aagaaggatg tccagtttca gctataacag ttaaatagag ctgtttaaat ttatttttaa
     7621 aataaatata taagatttaa ttggtactta agctatagta ttagaaattt ctattactat
     7681 agctttttaa aatagataaa cttaatatat aagatattac tttttacaaa tatggaggac
     7741 tgaaatatga aaaaaaatta tgaaatagta ttattcgatt tagatggaac acttacagac
     7801 cctaaagaag gaataacaaa gtctattcag tattctttaa atagttttgg cataaaagaa
     7861 gatttggaaa acttagacca atttatagga ccaccattac atgatacttt caaggagtat
     7921 tataaatttg aagataaaaa agctaaagaa gctgttgaaa agtatagaga atattttgct
     7981 gataaaggga tatttgaaaa taaaatttat gagaatatga aagagatatt agaaatgctc
     8041 tataaaaatg gtaaaatatt attagtagca acttctaagc caacagtttt tgcagaaact
     8101 atacttagat attttgatat agatagatat tttaaatata tagcaggtag caatcttgat
     8161 ggtacaagag tgaataaaaa tgaagttata caatatgtac tagacctatg taatgtaaaa
     8221 gataaagata aagttattat ggttggagat agaaagtatg acataattgg agctaaaaaa
     8281 ataggtatag attctattgg agtattgtat gggtatggtt cttttgaaga gattagtgaa
     8341 agtgaaccta cttatattgt cgaaaatgta gagtctataa aagatatttt gttataaata
     8401 tcttttatag acaaaaatat tacaaaatgg tgtcgcatat ttaaaaatat gatattatag
     8461 ttaaggaagt catttaaaaa agaggaggat gcacttaaaa tgaaatctat tttaattatg
     8521 ggtggaagcg acttcatagg ttctgcactt gctaagcgct tgattaaatg tgggtatcaa
     8581 attgatatac ttacaaatgg taaaaaggaa atagattata atggttttaa agaacatcta
     8641 atttgtgata gaaaagttcg aaaagatatg gaaaacatta tcacaggaag aaaatatgat
     8701 tacatatatg atatgacagc atatacaaaa gaagatgtaa gtaatctgat tgattttata
     8761 agcatggata acttaaaaaa gtatatagta ttgtcagcag gagcagtata taaagatagt
     8821 ggaagaaata taaaagaaga aaatgagaaa ggtgaaaatg aaaactgggg aaaatatggg
     8881 ttaaataaaa aagaggctga agattttatt ataaatagcc ctataccata tataattatt
     8941 agaccaactt acatatatgg tgaaaataat aatttatata gagagtatta tttctttgaa
     9001 aaaatagaaa aaaatgaaaa gataccagta ccaaaaggaa aacaagttag taatcaattt
     9061 atatatattg gggatttggt aaaggtattg gaaagcataa tgaaaaatcc tcatgtaaga
     9121 gaggcctata atgtgacaaa tccacagtta atttcttggg atgaccttat atatacatgt
     9181 ggagaaatta ttggcaaaga acctataata aaatatgtag atatggaaaa agtagagttt
     9241 agagaaagaa cgtatttccc atttagaaat atagacttta atcttgatat aaataagctt
     9301 attgaacatg ggctttatat accaaacgtg ttactaaaag aaggtcttac agcaacatat
     9361 aaatggtttt cagcaaataa gccaaaaatg catgacagaa aaatgaacaa agttgagtct
     9421 gtaatgcaaa tagtttaaaa aaataaataa gtaggtagat attttgaact caaaaattaa
     9481 gtatttttta aaatttttta tcttaatatt tattttgatt tttatatact cagttgcgat
     9541 agagccgagt ctattaattg tgaaaaaata taatataagt gaaaatggag cattgataaa
     9601 aaatgattca attgagagta aatctgaaag tgtaaaagta gttcaaattt cagatactca
     9661 aataggtagt ttttattcaa ctaagaatct caaaaaagta gctaataaga ttaacacttt
     9721 gaatccagat ataatagtat ttactggtga tttaattgat tactccaata aaaatccaag
     9781 tgtagatgaa attacaacta ttttatcaag tatgaatgct aggttgggaa aattttcagt
     9841 atttggcaat catgattata tgtataaact tcctaggtat tataggcaga ttatgaaaaa
     9901 ttccaacttc aatcttttag taaatgaaaa taaaaaaata aaattaaaag atgataaata
     9961 tattaatata ttaggtgttg atgaaatatt aaatggaaat cctaatataa aatatctgga
    10021 gagtcaaata gataataaaa gttttaattt attgttagca catgaaccag atttagtaga
    10081 tatgcttagt aaagatacaa tgaatttagt tctatctggg catagtcatg gtggtcaaat
    10141 aagattacct attaagggag cattagttac tcctccatat ggaaggaaat atactaaagg
    10201 gttttatgat ataaatggaa atcatttata tgtgagttcg ggtcttggaa gtacaaagtt
    10261 accatttaga ttttttaata taccagagat aatagaattt aatattataa tctaacataa
    10321 aatatttaga aaatataatt ttgcttttta taatatgtag atgtatatac tgtaaaaatg
    10381 gattaaacca gtagattgaa tgaattaata gattaaatta atatataaaa tatttaagtt
    10441 ata
//
LOCUS       DAEMIK010000146        10190 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEMIK010000146 DAEMIK010000000
VERSION     DAEMIK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.148"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(221..1471)
                     /locus_tag="KPK87_003147"
     CDS             complement(221..1471)
                     /locus_tag="KPK87_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG2539270.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1504..2445)
                     /locus_tag="KPK87_003148"
     CDS             complement(1504..2445)
                     /locus_tag="KPK87_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG2539271.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFFDFNNLIGVAL"
     gene            complement(2685..3761)
                     /locus_tag="KPK87_003149"
     CDS             complement(2685..3761)
                     /locus_tag="KPK87_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2539272.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4134..4649)
                     /locus_tag="KPK87_003150"
     CDS             complement(4134..4649)
                     /locus_tag="KPK87_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG2539273.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5012..6082)
                     /gene="leuB"
                     /locus_tag="KPK87_003151"
     CDS             complement(5012..6082)
                     /gene="leuB"
                     /locus_tag="KPK87_003151"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG2539274.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6089..6580)
                     /gene="leuD"
                     /locus_tag="KPK87_003152"
     CDS             complement(6089..6580)
                     /gene="leuD"
                     /locus_tag="KPK87_003152"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG2539275.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6595..7872)
                     /gene="leuC"
                     /locus_tag="KPK87_003153"
     CDS             complement(6595..7872)
                     /gene="leuC"
                     /locus_tag="KPK87_003153"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG2539276.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAIIGKISKPSEII"
     gene            complement(7829..9571)
                     /gene="leuA"
                     /locus_tag="KPK87_003154"
     CDS             complement(7829..9571)
                     /gene="leuA"
                     /locus_tag="KPK87_003154"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG2539277.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9695..9941)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3072 a         1980 c         1059 g         4079 t
ORIGIN      
        1 acatttaaaa ccttataaat atacaaatac aattatatga attttcatcc tcaattatag
       61 catagaatat gactatctat atcctaaaat tatccatttc taaaaaaaag gtaactatta
      121 ttaagaatat caacttatag aatttatatt ccacatacga ttttcaatca aaaaaacaag
      181 ggctaaaaag tgaattgtgc actttttagc cctttattaa ttattcattt atttcatgta
      241 agaatagacc acatcttact ttaggttcaa accatgttga cttagctggc attatcttat
      301 ttgcatctgc tactcttatt aaatcttcaa tactagtagg atacattgca aatgctactt
      361 tcatatcttc attaactctt ttttctaatt cttcaagacc tctaattcca ccgataaaat
      421 caattctttt atctactcta ggattatcaa ttcctaaaat agcatcaata acatagtctt
      481 gtaatataga aatgtctaat gagtcaacag ggtcttctat ttctaataaa tttttatttg
      541 ctttcatttt ataccactta tcacctaggt acattccgaa agttcctttt ttgtcaggtt
      601 tatatttttc ttttccttct atttcttcta gttcaaaatt ttcttttatt ttatttataa
      661 attcttcctc gcttaaacca tttaagtctt tgataaccct attataatcc aaaaccatta
      721 aattctcatc tggtgcaatc attgctatga agtaattaaa ttcttctttt ccagtataat
      781 ttggattttt agctctcatt tctttggcta cattttctgc tgaagctgtc ctatgatgac
      841 catctgcaat gtatagataa tctaattttt caaatttatc tactaggtta tctattatat
      901 catcattatc aatcttccat actgtatgct ctatcttgtc atctgttatg aaatcatata
      961 aaggttcatt attatccata aaatcattga ttatatcatc tatcaccctt tgatgtttgt
     1021 aagttacaag tatagttccc gtattagcat cacagtattt tatgtgttta gttctatcaa
     1081 tttccttttc tggtcgagtg tactcatgtt tttttattgt tccattcaag ctttcatcaa
     1141 ctgatataca tgctactata ccaacttgag ctctaccatc catgatttgt ttatatatat
     1201 atatacactc tttgtcatct tttactagaa tttcttgttt tcgaaactca tctaaatttt
     1261 ctcgcgcttt taaataaact ttttcatcat ataaatctat attttcatct aaatcaattt
     1321 ctgatttatc tatatgcaaa aatgaatatg gattcccttc tgcaattctt ctagcttctt
     1381 tactattcat aacatcgtaa ggaagtgctg ctactttatc tactatatat ttatttggtc
     1441 taatcgcctt aaatggttta atcgttgcca ttactaattt cctccctaat tatacaaaat
     1501 attttataat gctaccccta ttagattgtt gaagtcaaaa aagaaatctt gaatcacatt
     1561 tactatttcg tctcctattc tttcttgagc ttctactgta gatgccccaa tatgaggtgt
     1621 aggagaaact cttggatgat ttactaagtc taaatttaat gtaggttctt gttcataaac
     1681 atctatagca gctgctgata atttaccttc atctaatgct tttagtaaat ctttttcatc
     1741 aactaaaccg cctcttgcac agtttattaa ataagcccct ttcttcatct tatttatttc
     1801 tttttcagtt attactgccc ccttattctt atcaaatggt atgtgtaaag ataagaaatc
     1861 tgcattttct aaaacttctt ccatatcaca gaatttataa ttgttaaagc cttgcgcctc
     1921 acccattatg tcagtatata taacattcat acctaataat tctgctctct ttgcaacttc
     1981 ttttgcaatt cttccaaaac ctataagtcc aagtgtcttt ccattaattt ctgtaccttt
     2041 atactttttc ttctcccatt ttccatctct catagttaca tttgcagtat ttatgaatct
     2101 tgcaagaaca aataattgac ctattgtaag ctctgcaaca gatatactgc ttgcatttgg
     2161 agtattcata acttttatgc cattagcttg agcatattta acatctatat tatccagacc
     2221 tactccacct cttactatca acttcaactt tttaccctct aatgctttat ctattacatc
     2281 tttagtaact ttagtagctg aacgtactac aagtacatca acatctttaa gtttatctcc
     2341 taaaacatct ttttcataaa actgctctat cacattaaaa tctaactctc tcaattttct
     2401 cgctgcttct ttctctattc catcagtaac taatatatta tacataacaa actcctccaa
     2461 tttttattca cttaattttt ccaattttta ttttttaaag tattttcatc aataaaaaac
     2521 tttacttaat ttaactttct gtttatataa ttttcaaaat atttttttat taaagtcata
     2581 tcaaattttc ctattgtttg ttaaaaattt aatatgtaat ttttataaaa tattatttat
     2641 tatatattct aaatttatct taaaacatac atatttttac aatattattt attaaaacct
     2701 aatatatcat caatattatc taataattct tgtacatctt cgacagtata gtcacccata
     2761 tgtgaaattc taaaagtttt ttcttttaaa tctccatagc catttgctat ttggaatcct
     2821 cgttcttgta attttgaatt taaatttgat acactaatac cttgagtatt ttctataaca
     2881 gtaagtgtat tggataagtg attttcattg gtaaaaattt ggaagtgttt tttagcccag
     2941 tttctaacta agtttgccat gtcttcatgt ctgttgaatc tgttatctag gccttcatct
     3001 aatatattat ctaattggaa atctaaagcg aacatgtgag aaagagatgg tgttgaaggg
     3061 tattggtagt ttttcttgat taagtattca tacattgcta acaaatcaag atatactcct
     3121 ctaaaaggta cttgcttagc tctatcgatt gctttttgag aaaatgtgca aatagacatt
     3181 cctggtggta atcccagtgc tttttgtact gatgttatac aaatatctat tccaatctta
     3241 tcaacctcga cttttattcc accggctgaa cttacagtat ctacacagta gattacatct
     3301 tcatactttt taacaacttc tccaatttct tctattggat ttcttattcc agttgaagtt
     3361 tcattgtgag ttacagttat taaatcgtat tctccggtag aaagcacctt atcaaccatt
     3421 tctggcgttg ttgcctctcc taattcaact ttaaatatat ccgctgggac attattagcc
     3481 actgccattt tgtaccatct atcaccaaag gatccacagg aaaatactgc tgcctttttg
     3541 gatgtacagg aacgaatgga gccttccatt aaaccagttc cggatgacgt ggataaaagt
     3601 atcgtgttgt ttgtatagaa tagtttttgc atcttttcac ttatactctt ttggagcata
     3661 gatgcatctt ttcctctatg tcctatcatt ggtgtagcca tcttttgcaa gacttcttct
     3721 ttaacatcaa taggaccagg aataaataat ttcttactca ttataattcc ccccttttta
     3781 gtttgtaata tggatagcgt ttttactgtc cttattttta ataactataa tatcaagctg
     3841 aattaactat gtcaacataa aatttatttt ttatatataa tttattttat atattttatg
     3901 aaaacattca ttttctacct tttcataagt ctgaattttc tgaattattc tctcaaaaaa
     3961 aatatacttt ttatctatat aaattaattt ttttagtatg taaatttgac gctattacga
     4021 taaatctttt tagtataact aatttatttt tatcttgtat ttttattgta tttttaaatt
     4081 ttattttttc taaatataaa atttatgttt taaaatgtaa aatttttatt taatcaaagt
     4141 tcaatatatt ccactttttc tttcgattct tcaaaaaacc attcttcatt agaaaaatca
     4201 ccttctgcaa tttgtccaat aggcattttc atatactttc cagttgctgt cactgctata
     4261 tctccatttg gtagaattat ttctcctgta ccttcaaata attttctgtt ttctttagtt
     4321 attcttccaa caacttttat agtagcatct gttggtactg gtttcttata tttcaactct
     4381 aaagatactg ttactcccca tatttggtca tcatttatac ttacagctct tccaatagtc
     4441 tcatccaata ttgcagcaga catacctcca tgaactctcc caggataact ttgatgccaa
     4501 tcctttgtat tacaaattga tacaagctct ccattttcaa gttcataaaa atctgcttta
     4561 agtcctagct cattttgtgt tccacaaact aaacaagatt tactactatt ttgcttcttc
     4621 aaaactttat actttgccaa aaaatccatc tcctaaccga cattagtagt cgcttaatat
     4681 taaataattc taaaatatat tatataatat taataaaatc cctgcaaact tctaataata
     4741 aaaataggct acttcaatat gataagttta atttaaagta ttattttcca aaaaataact
     4801 ttatcaaaat agaaactcaa agtagatata agcctctttt tcgttattaa aatagtaaaa
     4861 atatatttta cttaatataa aaaagagtgt ctcattaact aataaagttt attttgagac
     4921 acccctcata aaacaaatta tactacaata gatttttact aattctacaa gttatatatt
     4981 taaattatat tattcaaatt aaactataat tctaaatttc atttactatt aattcgccca
     5041 ttgctcttgt tcctacaaca tttcccacac cattatagat atcaatagtt ctataacctt
     5101 tattgagtac actttgtaca gcatcttcaa tacacttagc ttcttcttct aagttaaaac
     5161 tatgtcttaa catcatgctt acagatagaa tagtcgcaat tggatttaca atatcttttc
     5221 cagctatatc aggtgcactt ccatgtattg gttcatacat accaaaacta tcttctctaa
     5281 gtgatgctga tggtaacata cctatagaac ccgtaatcat agatgcttca tcagatatta
     5341 tatctccaaa tatattatta gtcactatta catcaaattg gctagggtct tttacaactt
     5401 gcatagcagc attatcaaca tacatatgtg acagttctac atcttcaaac tctttagcta
     5461 agtcttccat tatacttctc caaagtcttg aactctcaag cacatttgcc ttatctacac
     5521 ttataagttt tttatttcta tttctggcta tttcaaaagc tctcttacca attcttctta
     5581 tttcattttc gttatatatt tctacatcat aagcattttc gataccatca atgatttttc
     5641 ttccacgttc tccaaaataa atgccccctg ttaattccct tacaacaaca aaatcaatgc
     5701 ctttctcaac tatatccgtt ctaagtggtg aagcttcttt tatactttca tacattgtag
     5761 ctggtcttaa atttgcaaat aaacccaatg actctcttaa ttttaaaaga ccagactctg
     5821 gtcttttatc tcccttacat ttatcccact ttggtcctcc aacagcacca agcaatacag
     5881 cttcgttttt tcgacaagtt tcaacagttt tattaggtag aggttctcca gtttcatcaa
     5941 tcgcgcatcc tccagctaat acatattcac aatcgaatct atgattaaat ttacaacaaa
     6001 ttttatttag aacctttata ccttcatcta taatttccgg tccaacacca tcacctttta
     6061 tgactgctat attacaattc atacttcact accccctatt tgcttttatt gagttaatta
     6121 agccttcatt taatataata ttttgcataa actcaggaaa tggttctgct ttatattctt
     6181 ttccttttgt tatatttttt atcacacctg tagaaaaatc tacttctata ttatcccctt
     6241 catctatgtt attggcagct tcttcacatt caagtatagg aagccctata tttatagagt
     6301 ttctaaaaaa tattctagca aaagtagaag caatcacaca ggatactcca ctttctttta
     6361 ttacaattgg tgcatgttct cttgatgaac cacaaccaaa gtttttagtt gcaactataa
     6421 tatctccttt ttttacctta gaaataaatt tatcatcaat atcctccata caatgcgttg
     6481 ctagttcttt ataatcagct atatttaaat atctagctgg tattattaca tctgtatcta
     6541 tattatcacc gaatttaaaa acacttccat tggcaatcat aaattttacc tcccttaaat
     6601 tatttctgat ggttttgata tttttccaat tatagctgat gctgctgcta cagctgggct
     6661 tgcaagatac acctctgaat caacatgccc cattcttcct acgaaattcc tatttgttgt
     6721 tgaaatggat ttttctcctg ctgcaagtat acccatatgt ccccctagac atggaccaca
     6781 agttggtgta gaaactgctg ctcctgcctc tataaaagtg gtaatatacc ctttttctat
     6841 agcttcaaga tatacttttt gagttccagg gaatatcata actctaactc catctgctac
     6901 ttttttgcct ttcattatct cagctactac ttcaaggtct gatattcttc catttgtaca
     6961 agagcctatt acaacttggt ctatctttat atccttatct atctcatcta ctgttctagt
     7021 attttcagga agatgaggaa aggctactgt ttcttttagt ttggctaaat ctatttcgta
     7081 tacttcatca tagactgcat cttcatctgc ttcatatata tttacatctt ttgtcatagt
     7141 tgaacactta tgtgaattta tatattccat agtcttatcg tctactggaa aaattccatt
     7201 ttttgctcct gcttctattg ccatattaca aatagtaaat ctatcatcca tagataagta
     7261 ttctactcca tctccacaaa attccattga cttgtaaagt gctccatcaa cacctatctc
     7321 ccctattata tgaagtatta tatctttacc acttatccat ttatttggtt tattcttcaa
     7381 aacaaatttt attgctccag gaaccttaaa ccaacatttt cctgtagcca tacctgctcc
     7441 catatcagtt gaacctacac cagtagaaaa tgctcctaat gctccatatg tgcaagtatg
     7501 agagtcagca cctataatta catctccagc agttactata cctttttcag gaagaagaac
     7561 atgctctatt cccatatctc ctacatcaaa aaaatttaca actccttttg attttgcgaa
     7621 acttcttgta aatttacatt gttctgcact ctttatatct ttattaggtg taaagtggtc
     7681 taaaaccatc actactttag tcttatcata aacatcttca attcctaatt tttcaaattc
     7741 tctaatggct actggtgatg ttatatcatt acctaaaact acatctaaat ctgcttcgat
     7801 taattgccct ttcttaactt catttaattt agcatgtttg gctaatattt tctgcgtcat
     7861 agtcatcccc attttttatt tcctcctcaa tataactttt atttattgca ttcataagtg
     7921 cattaatagc tgctgtattt atgttttccg atatacttac accaaaatag acttcattat
     7981 tgtgctctat tcctacataa gctacccctc ttgaatgtga cccttcctct aatgcatgtt
     8041 cccagtaata tctaaattta taatctttca tgccacattg ctttagtgca ttattaaatg
     8101 aatctacagg tccatttcct ataccaacaa tcctttgttc atttcccata taacttattc
     8161 ttgcagtcat ctcaatcata ttagtatcat tagaatcatc attttcatag tttaattcat
     8221 ccattgattt tattttatac ttcttaactt tataagggct ctcaacttca atatattctt
     8281 tcttaaacaa attaaatatt gcttcactac taagttcttc cccaattcta tcagattcca
     8341 ttttgactac atttccaaaa tcagaatgca tatttttagg tatcataaat ccataatctg
     8401 tttccattat ataaacagct cctccttttc cagattggct atttatcctt ataatttctt
     8461 tgtattctct tccaatatca tgagggtcta ttggtaaata tggaacttcc cagtaatcat
     8521 tatctctgtt tttcattgct atcatgcctt ttctaatagc atcttgatgt gaacctgaaa
     8581 aagcacaatg tacaagatta cctgcatatg gatgtctatc atgtaccata agttttgtac
     8641 agtcttcata tatacctatt atcttatcta tattgctaaa atctagctct gggtctattc
     8701 cttgtgtata aagattaagt cccacattta ctatatccat attcccagtt ctttcaccat
     8761 ttccaaatag tgttccttca agtctatcgg caccagccaa taatccaagt tcacttgcag
     8821 ccgtacaagt tcctctatca ttgtgagtat gcaaacttag tataatagaa tccctacaac
     8881 ttatattttt acaaaaccat tctatttggt ctgcatatac gtttggtgtt gccatttcaa
     8941 ctgttgatgg aagatttatt attacttttt tttgaggagt tggtttccaa acatctatca
     9001 ctgcctcaca tatttcaagt gcataatcca tctctgttcc tgtaaaactc tctggtgaat
     9061 attcaaaagt aaattctgtt tctggatatt tttttacttc ttcattaaat aattttgctc
     9121 cttcaactgc tatatctatt atttcttgtt tactcatatt aaaaaccaca tctctttgaa
     9181 gaacagatgt agagttatat aaatgtataa ttgctttttt acatcccctt attgattcaa
     9241 atgttttttc tataagatga gccctagatt gtgtaagtac ttgtatagtt acatcatctg
     9301 gaatcatatt ctcatctatt aattttctta aaaatgtata ctctgtattt gatgctgatg
     9361 gaaatcctac ctctatttct ttaaatcctg tatcaataag cattttgaac attctgactt
     9421 tttcatttac actcataggt gttggcagtg actgatttcc gtctcttaaa tcgacactac
     9481 accaaattgg agccttaaat atttcattat ctggccattt acgatttact atttgcattt
     9541 tatcatattt tttatacttt ccacacttca tgttatttcc ccctttttct gttttttgta
     9601 tttagtatac ctcatatcga cgcaatcctg cacaattcat aaggataaat gtcaatttta
     9661 tcattagaat aaaaaaaacc tcatctctaa taaaattaga gacgaggttt tgctcgcggt
     9721 accactctat ttagctttct aaaaagctcc cttagtaata gccaatacaa taattataat
     9781 gactatattt cctataacgt ggaattactc cgattattct aagatttaaa tcttagaaaa
     9841 cagctcctag acaagttcag aactatcaac tactaaatca caccatcctt tagctctctg
     9901 aaagtttcat tgttcttact acttctattc atcacttttt taatcattta aattttctaa
     9961 atattttgat ttaatagtta ttatatacac attaaaatgt attgtcaaca caaaaaataa
    10021 ttatctcaat tttttatttt tgataagcaa ttctataaaa catgctaact attgaaaaca
    10081 gtatgatatt atgccttaaa ctttttcatc ttttcatcta tgatattcaa taattttttt
    10141 tcatcttcca aactcaatgc ccttaatcta attttataat tagattcgtt
//
LOCUS       DAEMIK010000147        10182 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEMIK010000147 DAEMIK010000000
VERSION     DAEMIK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.149"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..860
                     /locus_tag="KPK87_003155"
     CDS             <1..860
                     /locus_tag="KPK87_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2539278.1"
                     /translation="YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENEN
                     LSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSL
                     STTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFK
                     IKELVKSFIKLTNINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIF
                     ITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFP
                     KFSGGDFFE"
     gene            853..1500
                     /locus_tag="KPK87_003156"
     CDS             853..1500
                     /locus_tag="KPK87_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539279.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1629..2564
                     /locus_tag="KPK87_003157"
     CDS             1629..2564
                     /locus_tag="KPK87_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539280.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQISGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2578..3675
                     /locus_tag="KPK87_003158"
     CDS             2578..3675
                     /locus_tag="KPK87_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539281.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3678..4742
                     /locus_tag="KPK87_003159"
                     /pseudo
     CDS             3678..4742
                     /locus_tag="KPK87_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            4986..5483
                     /locus_tag="KPK87_003160"
     CDS             4986..5483
                     /locus_tag="KPK87_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG2539282.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5659..5907)
                     /locus_tag="KPK87_003161"
     CDS             complement(5659..5907)
                     /locus_tag="KPK87_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539283.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5973..6989)
                     /locus_tag="KPK87_003162"
     CDS             complement(5973..6989)
                     /locus_tag="KPK87_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2539284.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7047..7814)
                     /locus_tag="KPK87_003163"
     CDS             complement(7047..7814)
                     /locus_tag="KPK87_003163"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG2539285.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7882..10071)
                     /locus_tag="KPK87_003164"
     CDS             complement(7882..10071)
                     /locus_tag="KPK87_003164"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG2539286.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3531 a         1568 c         1188 g         3895 t
ORIGIN      
        1 agtacatatt gttctctttt gttataatat cttctcttat tattagttta gataaaaata
       61 tatatttggc actggagtca agctctatat tgctaattat aactgtactt tctatatata
      121 tagaaaatga gaatttatca aaattataca gtcaaaatct ttatgataag cttagaattt
      181 ctaaagatga attaaaaaaa gttaatgctg atttggaaac ttatgcaagt tctatagaag
      241 aattagcaat tttaaaagaa agaaatcgaa tatcaagaga aatacatgat agtgtaggac
      301 attctctttc tacaacaata atacaattaa atgctataga aaaactctta aaagacaaac
      361 ctctaattta tgacttagta catgaactta gagaatttgt aactgaaagt tttcaagatg
      421 taagaagggc tatatctgag ttaaaacctg ttgaatatga aaattaccaa agtttattta
      481 aaatcaaaga attagttaaa agctttatta agcttacaaa tattaatgtt aaattaacta
      541 tttcaaaaaa tacttggaac ttatccagaa atcaatctat agctttatat agacttatac
      601 aggaatccct ttccaactca tctaggcatg gaaaggcaac tgaaatcaga atttttatta
      661 cattcaatac ttcaaatctt ataattacta ttagtgataa tggtatagga tgtggcaata
      721 taaaaaaagg aaatggactt aatagtatca atgagagaat atatgaatta aatggtaaag
      781 ttgaatttga taattctaat aatggtttca caattagagc ttcttttcct aaattttcag
      841 ggggtgattt ttttgaataa aattaaagtt cttatagtag atgacgaaaa attaattaga
      901 aaaggtctta aaattatatt atcatcttat aatgatttag aaattgtagg agatgcttct
      961 aatggatatg aagctttaga attttgtaaa atgaatgatg tagacatagt tcttatggat
     1021 atacgaatga aagtatgtga tggggttcta ggtacaaggc ttataaagga atataataat
     1081 tctataacat tattaatttt aacaactttt aatgatgatg aatatataaa agatgctatg
     1141 aagtttggtg cttcaggata ccttttaaaa gatagctctg atgaagtatt gcatgaaggg
     1201 attcgttctt cattttttgg caatattgtg cttgataaaa gtgtagctga aaaaatcatg
     1261 acctctgaaa aaacggttaa acaagagcat ctttatgaca tgtacaattt aacagaaaaa
     1321 gaaatatcta taattagact aattgcaaat ggacttaata ataaagaaat aagtcaagaa
     1381 ctattcttat ctgaaggtac aataaagaat aacataacta atattttagc taaactagaa
     1441 cttcgtgaca gaactcaact agcaatcttt gctttcaaaa ataaaattgt aatagaataa
     1501 atctaaaaat ataaagtgac ttaagtcact ttttttagtg acttatagta atagaattat
     1561 aattcacaat tctttattat aatattatag ctttttaact tatacaaaat tattttggag
     1621 ggattactat ggatatagtc agagtaaata atataacaaa aagatttaat gataaattag
     1681 tacttgataa catcagcttt tctgttaaaa aaggtgagat atttggactt attggcccaa
     1741 atggtgcagg taaatctaca cttataaata taattacaaa tcttatgtta ccaaatagtg
     1801 gtagtattca aatcagtggt ttagatttat caaaagatta tataaaagca aagtctataa
     1861 tagggttagt tccacaagaa ttagcaatta tagaaactct tactcctttt gataacttag
     1921 agtactttgg agctttctac ggtttaaagg gaaaactact taaagaaaga atcattgaag
     1981 cattagaagt tacaggtctt actgaagtca aaaagaaaaa ggtcaaaaaa ttatctggtg
     2041 gtatgcaaag gagattaaat attggaatag cactcttaaa tcatcctaaa atattaatat
     2101 tggatgaacc aactgtaggt gtagaccccc aatcaagaaa tcatattttc aattttataa
     2161 aagatatatc taaaaaacat gaaactactg taatatatac ttcccattat atggaagaag
     2221 ttgagcacct ttgttctaaa atttttatta tggatgaagg taaagaaatt gcttttggag
     2281 ataatgatta tttaaaatca ctcgtatcta caaatacaaa attaattatg gaaataaaaa
     2341 atataaatgc ccaacttatc tttgatttaa aaaatactaa aggtgttata tctgttttag
     2401 aaaataattc attattagaa ttggatatag ataaaaaatt acaacttaca gatatactct
     2461 ctgtcattga caaaaacaat tcaaaaataa tgaaaatttc ttatgaagaa cctagcctag
     2521 aagacgtatt tttaaatcta acaggaaaaa atttaagaga ctaatggagg tgattttatg
     2581 aaattataca tgagtgtaaa aactatgcta aagagtttaa aaagctcctt tatacttaat
     2641 ttaatatatt ttttagcttt gcctctgatt ttatcttggt ttcttgggat ggttacagaa
     2701 agcatgtttc aaaaccctat aaaaacagaa tctaccccca ttgttatata tgacaaagat
     2761 aacacaagat tatcaaatga tttgacaaag tatttgaaaa atgatttatc ttatatatta
     2821 acagttaaaa aagatgattc taaggcagaa ttaaagctta ctatacctaa aggctatgag
     2881 agtagtttat taaatgaaaa atcaaatact ctaaacatag aaaaacttgg aactcgtgat
     2941 gatatcgcaa tattgcttca agatatatta gatacttacc atgaaaaatt ttatttaaat
     3001 aattctcaga aaataagttc agaagatttt tctaaattat ttaataaaaa ttctattgat
     3061 acttctataa taaataacaa tgttaaacag agctcttatg aatactttgc tttagtttca
     3121 ctaggatttc ttgtaataat ttttataatg aacaatattt tatcaaatta tataagtgaa
     3181 tcaaagggac tttcaaaaag actttattct atgcctataa ctagagtaca attcttaatt
     3241 tatgactttg taggtttatg gatttattca tttatattct tattattata tgttttattc
     3301 tttagaatac ttggaataac atttaaaggt aattttgcca tattgctcct actttgtgct
     3361 ttatcatctt attttatgac ttcaatatct acctttgtaa ccagcttttt tagcaaaaaa
     3421 tacggtacag caatagttta tgcattactc tttttacaga caatttttgg aggtatcttc
     3481 agtatgataa gtgatacatt tactaagctt acaagtttat ctcctaccta tttaatagga
     3541 gaattgttta gtaattatga aacatttaaa actatagatt caataggaga tttaattttc
     3601 acatgtttaa ttatctctac aatattaatt gcacttgcta ttgtaaagga aaaatataaa
     3661 tggagggagg tttaataatg attagattaa ctattgcaaa tataaaaaga tatctaaaaa
     3721 atcatactct tttaattaat atggttatgc ttcctattat actaattttt tcacttaatt
     3781 tttttatcaa caactcggga aatcaatcaa tgtatttttc tccagtagca attgtatctg
     3841 actcttcagg aaagtatgaa cataagttaa taaatagttc taaattaaaa gaaaattcat
     3901 ttagattaaa tgaacaagat aaggctatga atctattaaa aaataataag gtttctgctg
     3961 tattcgtttt agataaaaat ttttcaaata gtatagataa attacaacga cctgtagtta
     4021 aatgctttaa aattgaaaat ggtggtggta gtctttgggc agaatcacaa atagaatctt
     4081 ttattactaa atcgttaaaa ttgaaagtgg ataaaaatat agatgataaa ttgacaaaga
     4141 caaatataat tgataacaaa ccagataaga ataaaggttc atttttagta gtcttcttga
     4201 tatgctattt tatgtatatt aatgcggcac atcttgcttc tgatttattt acattaaaaa
     4261 aatcaaatgt gctaaaaaga cttatctcta cagataataa agatataaaa ataattttca
     4321 gtatattttt aggactattt tttattcaat caattgtata cacttttgct ctattatgtt
     4381 tttcaattat agaagacttt aatttgagtc tgaatgtatt gatgattgta ctagcaaata
     4441 gttttgtttc cactggattt gtattttggg tagctagagt atttaaaaat gaaagttcta
     4501 taagtcttat atctacattt tattctttaa taggtctagc tatatctatc tctagtctaa
     4561 taccatcaat ggataaatta tcctttatga ctaatctttc aaagttcaca ccattttatt
     4621 ggactataga tgctatttaa aacaatggta gtattttaat aaatataata gctctaatat
     4681 taattggaat tatatttgta actgcaggaa gtcttaaact aagagatttt gctaaaaact
     4741 aataattatt aaatataatt agtatctatt tttatataat tgtaaaagaa tatttcaaca
     4801 tattaaatta ttttatctta tatttttctc tttagtttca cacatcacca agtctaaact
     4861 ttatattata tattaatatt tttgacttgt caagttattt atgttataat attaatcatt
     4921 agataattaa agcattgtac ctaagttgca atatctaaaa ttcaatttgg aggtataact
     4981 agcatatgta taaaattggt gaaagtgtaa tgtatccaaa agagggagcc tgctctgtaa
     5041 atgatattgt aacaaagaaa ataaatcatg agatgcaaaa atactatgaa ttaagtgtca
     5101 tttttaatag taaccttaaa atttcgattc cagttctaaa tgcagataga attggcatac
     5161 gtccagttat ggatggaaat gatgttgata attttatcca atctattaat aaaacagatg
     5221 gtgtatggat ttttgataga aaagaaagac ttaaactgta ccaagataaa tttcattcag
     5281 gagatgtttt tgaaattgta aaattaatca aaatgcttat gatacaagac agttcaaaac
     5341 aattgtgtag cacagataag gagtttttaa ataaagctca aaaatttgct ttatcagaat
     5401 tagctgctgc tcaatgtaaa tcctatacaa tggtattaga ggaaatgaaa aaacatatat
     5461 taaattcaaa gaatacaaat taaaccagtt ttttaaactg gttttatttt ttatactaat
     5521 catccatgct actttagctt attttaaact gcctaatcta atattaataa agttatctca
     5581 attagataga tgtattttat tcaaataaaa aaaactaaga gtaaatcata ttactgattt
     5641 actcttagct aataaaattt aatcttcttc aagttctttt ttctcttgta ataatacact
     5701 taattcattt gcttctaaaa cttgtgcctc tgcaatatca agtaactctt gttgccattc
     5761 cacacattta tctttgtaat caaaaggtag cgttctgtca actatgtact tatcaataca
     5821 gtcctccgca cttttaatta tttcttttct ttcatcaata aatttttcta attctactaa
     5881 ggcttcttca taagcttttc tactcatata aattccccct ttgtacaatt attctacatt
     5941 aaaatgattt ttcctctttt ttgtataaaa ttttatttgt atataacttt tccatcaata
     6001 tcttttatag tttgacatcc agttaatatc atagaactac ttagctctga ttgaagttta
     6061 tttacataag tttctacacc atcagttgca ccaccaaaag aagcagtcac aaaaggtctt
     6121 cctattaata ctgcatctgc acctaaacct atcattttta gtacgtctaa accagttctc
     6181 actccaccat ctgcaagtat tgtaacttta cctttaacag catcagcaat ttttggtaaa
     6241 acttcacatg ctcctggtgt acagtcaagc actcttcctc catgatttga tacgactatt
     6301 gcatccacac ctgcttctac tgccattaaa gcatcttcaa cagtcattat acctttcaat
     6361 ataaatggta gtttagtaga ctttactaac tcctttattt gtttaacatt tttaggaagt
     6421 actggttttc catgtaatga taaagttact agaccacagg catctatatc aactccaact
     6481 gcaaatgcac cagcttcttc agctaatctt atctttttta taatattttc attatcccaa
     6541 ggtttaataa atacaattcc agctccatta tactttttga ctacatctaa attttccatt
     6601 aaaaaagcat ctactgccgt atcaccaacc attgcataaa taccactatt agcacatcct
     6661 gcaactactg gttctatgta atctctttca ttaatttttc cacccatatt taaagtagtt
     6721 cctgttactg gagctgcaaa tataggtgca ctcattttct ttccaaacat ctctatagat
     6781 gtatcaggat ttgatacatc atgtattact ctcatattta cttttacttt ttctaaactc
     6841 ttgctatttt ctataaaaga agaaccactt cctttacctc ccatgccagg cacttcacct
     6901 gcacaagcaa ccccattaca aaccttacaa actttacaac ttccatttaa attttctcta
     6961 gcacttttta ataattcatt atattccatc tttaatcccc cattaactat agtaatttat
     7021 attatataca ttatatcata ttatatttac agtatatttt tcaaaaaact ttttctgtgg
     7081 ataggagtta tgccatattt ttcaatagct tcatagtgtt ctttagttcc atatcctttg
     7141 tgagatttaa acccatattc tgggtatact ctatcatatt ggtacattat actatcccta
     7201 gtaactttag caagtatact tgctgctgct attgatatag attttgaatc tcctttcaca
     7261 attggatttt gagatatgtc aatacctggt attgttgctg catcaactaa taaataatct
     7321 ggtgcttttt ttaaacaatt tatagccttt ttcattgcca tatatgtagc atttaatata
     7381 ttaaattcat ctatttcttc attgtttact atacctattc cataatctag tgcttcttct
     7441 tttattactt caaaaagttc atctcttttt gcctcactca gtttcttaga gtcatttaca
     7501 ccttctattt tggtatcttt tttaaatact acaacagaag ctacaacagg tccagcaagt
     7561 ggtcctcttc ccgcctcatc aattcctcca atatataaat aacctttatc atagccttcg
     7621 ttctcaaata tatttatagt ttccaatctt tcttcttctt ttctaatatt atccagcttt
     7681 tttgctagtt ttatagctag accttgtaca gattttcttt catcaactct taacagctct
     7741 atatattcca tatatttttc tacttccaaa gtctctatta tttctttaat ctccctcaca
     7801 cttttatcct gcataccatt ccctcatctt tcgtttttaa aaatttaatt tttaaatata
     7861 ttaaaatttt ttaattagag tttaattgca tccactatag ccacatttta aacatatact
     7921 acatccacct gtatgtgcaa ttccttcact tccacactca gggcacacaa atttttcatc
     7981 ttcttcaaaa tttttattta ttatttcttt ggctttttca tctttattat ctttttcttt
     8041 tttattattt ttataatatt cttttttact attttctttt ttatcttctt tattattttc
     8101 ttcctttttt tcatttatta aaattccact ccttttacat ccatctctaa atattgttac
     8161 tcctttaaga ccagcctccc atgccttaat atatatattg aaaacatctt caactgtagt
     8221 ttcaaaaggt atatttattg tagatgatat agaagcatca atatactttt gccatacttt
     8281 ttgcatgttt attctttctt catatcctat attcatagca gtaacaaaaa aatctggtaa
     8341 acattcttca tctgtaatac ctttcttgtc catatactct ttaacaattg gagtatatac
     8401 tttataatac acatcctcat catgcaagct ttcagttttt cttatatatg aaagattaaa
     8461 tataggttct attccaccac tgatacctat cattgtagat atggaacctg taggaggtat
     8521 ggttaaaagt tgtgaatttc taagcccata ctttttaacc atttccttag tgtcttcatt
     8581 tagattttct ataaaaaact tagatttaga aatattttca tatttatact cactaaatgt
     8641 tccatattct ttagcaatta atgctgattg cttcacagct tcatttaaca tagtcttacc
     8701 aattaaatca catagttcta aagattcatc agaaccatat cttattccca tttttataag
     8761 catatcagct atacccatta cacctaaacc tatctgtcta tacttatgta cactatctct
     8821 ttgttcttgt aatggatgca agttcaatcc ctcatctaag acatcattta aagctactac
     8881 agcttctcta acgcaagatt ttaatttatt aatatcaaaa gtagcatgtt caccaaatgg
     8941 ttgtattata aactctgaca agtttattga gccaagtaga caacttcctc cacttggaag
     9001 tggttcttct gcacaaggat ttactcctga aaagcaaaag ttcttatctt cactcataaa
     9061 attatatgtt tttattttat cccaaaacaa tatacctggt tctgcataat cccagttatt
     9121 tttcacaaat ttattaaata aatcataagc atttactatc ttaactattt tttctccagt
     9181 agtttcaact tcaaaagtac aattatataa ctttttttct cttacagctt gcataaattc
     9241 atctgttact cttacagaaa tatttgcttt agtaaccttc tccaaatctg tttttatatc
     9301 tataaagtct tctatctctg gatgatttac atccatagaa atcataagag ctcctcttct
     9361 ccctctttga ccaattaagc cagttgtcat actgtaaagt tccataaaag agactgctcc
     9421 agtcgtatgt tttgcagcat tgttaacttt cgctcctttt ggtcttagct ttgaaatatc
     9481 aattccaact cctccaccat aactaaaagt tctagcaagt ttttttgcag tatcaaatat
     9541 atcttctatg ctatcttttg gtggttctat tacataacaa ttagaatatg ttactttttt
     9601 tccttctttc tgtaatcctc tatttgctaa aattcttcca gcaaatagaa atcttttttg
     9661 tcttattagt tttgatattc ttgaactatt atttgaaatt ctctttatcc attcatcaaa
     9721 actctcacca ttatgtctat acttgttctt ccatattgat ttttgtaatt catcaaactc
     9781 ccaagcttta gtccttattt cagagcgtct ttctctatat aatatgtagt gttttgctat
     9841 atcttttcta tttgattcca taagtagtac ttcaactata tcttgtattt cttctacgga
     9901 taagcatttt tccatagaat tatagtcatt agctattttt cttgatatat catatgccaa
     9961 ctctctatct actccacctt cactatttat actagcttta tagacagcat cttcaatctt
    10021 acttttacta aaaagttctt ttgtaccatc tctttttatt atgtatttca tttttaaacc
    10081 tccaataaat tagtattaaa ttaatacaca cattaactat aactctaaac aaacttttta
    10141 tatatttgta tattttcata ttaattatct aacataatat at
//
LOCUS       DAEMIK010000148        10124 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEMIK010000148 DAEMIK010000000
VERSION     DAEMIK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.150"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            96..659
                     /locus_tag="KPK87_003165"
     CDS             96..659
                     /locus_tag="KPK87_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539287.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            1056..2756
                     /locus_tag="KPK87_003166"
     CDS             1056..2756
                     /locus_tag="KPK87_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539288.1"
                     /translation="MKRYLKTYGVALIIVIIYSFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2926..3543
                     /locus_tag="KPK87_003167"
     CDS             2926..3543
                     /locus_tag="KPK87_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539289.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3904..5097
                     /locus_tag="KPK87_003168"
     CDS             3904..5097
                     /locus_tag="KPK87_003168"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG2539290.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            5115..6407
                     /gene="lysA"
                     /locus_tag="KPK87_003169"
     CDS             5115..6407
                     /gene="lysA"
                     /locus_tag="KPK87_003169"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG2539291.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            6624..7283
                     /locus_tag="KPK87_003170"
     CDS             6624..7283
                     /locus_tag="KPK87_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2539292.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(7438..8523)
                     /locus_tag="KPK87_003171"
     CDS             complement(7438..8523)
                     /locus_tag="KPK87_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2539293.1"
                     /translation="MIIISRENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            8714..9430
                     /locus_tag="KPK87_003172"
     CDS             8714..9430
                     /locus_tag="KPK87_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG2539294.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            9488..10066
                     /locus_tag="KPK87_003173"
     CDS             9488..10066
                     /locus_tag="KPK87_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2539295.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
BASE COUNT         4170 a         1050 c         1756 g         3148 t
ORIGIN      
        1 aaaatttgta aaaccacatt tattatgtta gaatatctta tgatattaaa ttgtaagtgt
       61 ataatattat ttagttacta gattgggggg aaatgatgaa taaagtctat attgtaacaa
      121 catatacagg aactatatta tcttacttaa taaggaatat aagtaaaaag ctgtatacac
      181 atgtgtcaat atcattaaat gaaaatctaa aaccaatgta ttcttttgga aggctaaatc
      241 caaggaatcc atttattgga ggatttgttg aagaaaatat aaatcaagga ttatatgcta
      301 taagaaaaaa cactgtgtgt agggtatatt cgttagaagt agataattta caatatgaaa
      361 atctatataa aaatataaaa ttaattagtg attatagaga agattactat tacgatacaa
      421 tggcattaat atatttacta atgaatattc agagacaatc tgaatataaa tatgtatgtt
      481 caaattttgt tgctgacatg ttagaaaaaa gtgagataga cattttaaat aaacaagcat
      541 ttgaagtaac gccaaatgat ttttataatt tatctggctt aaccttagag tatgaaggtt
      601 tattatcaga atataattct agacaacatt cttatgtatc aaaaatcgca aacatttaaa
      661 aacttgattt agagtttttc atataaaaaa atattgctag ataattaggc tgaagatagt
      721 agtttaactt cgataggatt aagaatttgt aaatcttagt tagaatactt tcttcagttt
      781 ttaaatttct gctaatattt gtatttttat aatatttttg gatatatata atacagaata
      841 gtgcaaaaat cccactcctt taggtgcggg atggatagca caaacagtgt ataaaaagta
      901 tttatacata cacgtataat gttaaatata gttgaataag cagaactata aatatataaa
      961 gatatcttaa tatataataa atatgataaa tatttatttt ttgtaaatat atggatataa
     1021 tagtaataat aacatttttt agagaggagt attaaatgaa acgatatctt aaaacatatg
     1081 gagttgcatt aataattgta ataatttatt catttattaa attacctgta ctaagattag
     1141 attttacatc tttattttca gtaggaataa ttttctttgt tgtagcagga atactagata
     1201 tgatgttaga tagaaatgaa agagcatcta aaatggctaa atataatttt tcaatagcaa
     1261 tagctttgct tgtgtatatt gtagtagttc cctttataac ttctacacct gtacttcatg
     1321 caaaatcata tagagaacta ttgggaaaag taactgaaag taaatttact gatgacatta
     1381 gccctgtcag tgtaaatgac attcgtcttg tagatgaaga tatggcaatg aaattaggtg
     1441 acaaaaagct tggagaagtt cctgcaatag ggagtgtatc caaattagga aaatttcata
     1501 tacaaaatgt agatggagag ttgtactggg tagcgcctct tgttcacaga gacataatta
     1561 aatggataac atctctaagt ggtacaagtg gttatgtaaa agtatcagct agtaatcctc
     1621 aagacgtcca attagttcaa gaaatagatg gaaagccaat aaagatagtt taccaaccag
     1681 aagcctactt acatcaggat ttacaaagac atttatacat acatggtatt gtaaatgttg
     1741 gtatgacaga tttcactctt gaaataaatg atgaaggaag accttattgg gttgtaagtt
     1801 tatatgaaca taaaattggc tatggaggag caaatgcaac aggaatagcc actgttgatg
     1861 cagaaactgg aaaaataaat gtttatgatg ttaaaaacac acctaaatgg gtggatagaa
     1921 tacaaccaca aagctttgta acagaccaga ttaaagactg gggtgtttat gttaatggat
     1981 ttttaaactc tgttatatca gaaaagggag ttttagtacc aacagaaggt acatcattag
     2041 tttatggcaa tgataacagg tcttattggt atacaggtat aacatcttca ggtggagatg
     2101 aatctacaat tggatttatg ttagttgatt ctagaacaaa agaagctaga ttatataaac
     2161 aaccaggagc tactgaaacg gctgcaatga aatcagctga aggtaatgtt caagaaaaaa
     2221 attatgaagc tacgttccct gtaatgtata atatattggg tcagccaaca tatgtatcat
     2281 cattaaaaga taaggctggt cttgttaaga gagttgcatt tgtatctgtt gaagattata
     2341 atgtacttgg aataggtgaa gataagaatg aagcattaag aaactataag gatgcattag
     2401 aatctaaggg taatgattta aaacttgata atgatttaaa agatgaggta ttagaaggca
     2461 cagtgacaag aataagccca gatgtgcgag gtggtaacac taattattat gtaactttag
     2521 attcaaataa agatataata tttagggcta cttcaaaagt atctagcgaa ttgccattga
     2581 ctcaggttgg tgataaagtc aaaatatcgt attcaaaaga agaatctggt gtaatagaaa
     2641 tgtcagaatt tgataaccta aatattgcaa actttgaaga taaaaaagca aaagaaaaaa
     2701 caggaactga tgaaaataca gattccaaag attctgaaaa tcaaggtaat gtataattta
     2761 aataaaaaga ttgataatag tacttaacat agtgatagct aatttttata gaatttaatt
     2821 ctataaaaat tagctatttt attttaattc tttgtaaatt taatgtcata aaattaatat
     2881 aatattagta caactttgca aataatactt caaggagaat gtactatgac gaatatggaa
     2941 aaagagatat tacttttaat tagcagggtt agatgtgtta cagagtctca atttagaaaa
     3001 atttatggag accaaaaaag atacaataaa aaaaatttta aaaaaacttt gagaaaaatg
     3061 tgtagtgaat atacactaaa aaaatatcct tgtgatgtgg attattataa ttataaagac
     3121 ctctcttatg tatactattt aaacggtagt aaacttttta agggagatga tttaataaag
     3181 gctctagtag gatcagaatt agttgttaga atcaatactg caggttgtga tgtaaaaaga
     3241 ttttaccgaa atgttagtgt agatgatgtg aaatacgatt tatttattga atatacagac
     3301 caatttggta atataaagca agttttagtt gatgttgact taaataatga aatagacatt
     3361 tctaaatacg aaaatctttc taaaaaaata gataaatcta caattccatt ttttaaagta
     3421 ccaaacgttt tggtagttac accagataat ctatccgaat taaatgattt agagagatat
     3481 gataatgagt atagcataaa ttttatagat ttaggattaa ataaactatt taattgtata
     3541 taagctaact ttagaaaaga aaactatcga tttgccacat tttgtaaaat aattgttgca
     3601 atattatata gaatatgata aaactatatt aataaattaa ttaaaaataa tgagcctagg
     3661 gtagaggtag ctgtattcaa tagtattcaa taggagctgg taagcgttga taattgatga
     3721 aagggtatat agccgaagaa attttgttga cggacaaatt ttctggactt atgcagaata
     3781 tgcataaggc tgtcactttt aaagtggtga gctacaaggt tacttggtct atgaaattta
     3841 cacgtccaaa gtataccttt gggcgttttt atttatcaaa atttagcttg agggggagaa
     3901 agcatgagta taattgttca aaaatatggt ggaagttcag tagcagatac tgaaaaaata
     3961 aaatcaatag cagaaaatat aatagaacga agaaaagaaa atccacaaat ggtaatagtt
     4021 gtgtctgcca tgggaaaatc cactgatgaa tatataactt tagcaaagga attgagtaat
     4081 gaacctagta agagggaatt agatgcttta atgtctactg gtgaaatgat ttcagcatct
     4141 ttattatcaa tagcattaaa tgcacttgga tgtaaagcta taagttataa tgcttatcag
     4201 ttaaatataa aaacgagtgg tcttcatgga aagtctcaaa ttgatgatat aaacgtaaac
     4261 agaattaaaa atagtttaga tgaaggaaat gtcgtgatag taactggatt tcagggaatc
     4321 aacgaagatg gtgatgtaac cactcttgga agaggtggtt cagatacatc tgctgtagct
     4381 cttgcggtta aattaaatgg aaaatgtgag atatatacag atgttgatgg aatttatttt
     4441 acagacccaa gaaaatattc aaaggctagt aaacttgatg agattgaata tgaagaaatg
     4501 cttgagttag cgagtttagg agcacaagta atgcattcaa gaagtataga gcttgcacaa
     4561 aaatatggtg ttgaaatcta tgtcggacgt acatgtggta cagaaaaagg aacatatata
     4621 agagggggaa aagacatgaa attagaggat aaagtaataa caggattagc tactagtgat
     4681 gatgacagtt ctataacaat aaaagatttt aaagctgaaa atatatctag cttatttgaa
     4741 gatatagcaa ctataggtat aagtgtagat atgataagcc aaactgcacc tattttagat
     4801 aagataagtg tatcatttac agttccaaaa gaagagttgg gagaatgtaa aaagatagta
     4861 tctaaatata cagatgaaga acatgtagtg attgataata atatcactaa attttcttta
     4921 gtaggtcttg gtatgaaaaa tacttctgga gttgctgcta aagtatttaa gatattcaat
     4981 gaaaatggta taatgataaa acttataact acatcagaga ttcgtataac ttgtgctata
     5041 aattctgatg ataagcaagt agctatagaa aaaatagcag aagtatttaa tatataaaat
     5101 ttggggaggc tattatgaaa cttcatggaa ctatgactgt aaataatgat gaattatata
     5161 ttggaggagt gagttgttta gaacttagta aaaaatatca aactccactt tatgtatttg
     5221 atgaagcttt agttagacaa aattgtagag aatatataga atattttaag gctagagagg
     5281 gaaaaaataa ggttgcttat gcagggaaag cttttttacc tctttacatg tgtaatctta
     5341 taaatgaaga acaaatgtgt ttagatgtag tttcaggagg cgagttatat acagcttata
     5401 aatcaaattt tcctatggaa aaagttttct ttcatggaaa caataagact aaagaagaaa
     5461 taaacatggg attagattta ggtgtaggta aatttgtagt ggataatttt tatgaattag
     5521 atttgcttga agcattatgt gaagataaag gtaaaactca agagatatac tttagaataa
     5581 ctcctggtat agaagctcat acacatgatt atataaaaac tggacagata gattctaaat
     5641 ttggatttgc tttagtaaat ggagatttat ttagagcaat agaaaaatta agtgaatata
     5701 aaaatattaa acttgtaggt cttcacgctc atataggttc acaaatattt gatgtagaac
     5761 catacataga tacagtggat ataatgatga acttggtaaa agaaataaaa gaaaaattca
     5821 acatacaact aacagaaatt gacctaggtg gaggagttgg cgtttattat agcgaaggag
     5881 ataaacctaa aactataaaa gagttttgtg aaaccataat cactaaagca gaaacttcat
     5941 gtaaagagct tggaatagaa gtacctacat tggtaataga accaggaaga tcaatagttg
     6001 caaatgcagg aagtacattg tatacagtag gttctataaa agatataaaa gatgttaggg
     6061 tttatgtaag tgtagatgga ggtatgacag ataatgtaag accttcgttg taccaagcta
     6121 gttatgagtg tagtattgtg aataaaatta atcatgaggc tatgaatcat gtaacaatag
     6181 ctggaaaatg ttgtgagagt ggagatatat taattggaga tataaacata atggatatta
     6241 aaagtggaga tatacttata acaacatcaa ctggtgcata tggatattca atgtcttcaa
     6301 attataataa aataccaaga gcagcagtgg tatcagtatt tgatggaaat gataagataa
     6361 tttgtaagag acaaagctat gaggacttat tagctttaga gatataaaat agagaagtat
     6421 ataataaaaa ttaaatatta aagtttatta tgagcagatt tgctaggata tgtatctcta
     6481 aattaatttt tggtttattg tatcgttcgt agcatatctg cttatttttt gttttactaa
     6541 caaatttctt gtaataaata taaaatattt taaaaacttt ataattatga taaaatttaa
     6601 agtatatgac aaggagggat ttaatgagag gaagagtaat tttattaata tcgatattat
     6661 ctatatttct tgtaggatgt tcatttaata aaaaagatga aataaattta gtagagcaag
     6721 gaaaaacata tttagaaaaa cataactata aaaaagctat ggaatcatta tcctctgcac
     6781 tggaagaaga tagtactaat gagaatgcaa gagctatgta tatgcaagct atgagaatgt
     6841 caaatatgac ggagtttgaa gaattaaaaa attatgaggg tgcaatcaag gaacttaaac
     6901 ttatagagaa aatcaaaaat ggttcctctg ttattaaaga agaggcaact aaaaagaaag
     6961 aagagttaac taaattagaa aaagaacaaa acgaagcaga ggaacaaaga aagaaaaagg
     7021 caaaagatac ggctggagaa gataggtata gagttgaatc agatgcaagg aaatcttctt
     7081 atagtggaag tagtaaaaat aataaatcta gttctaaaaa taatgataaa aaatcagata
     7141 gtaataagtc tgacaataat aacagttctt ctcaaaatca agaaaagcca aataatagta
     7201 atccaacacc agcaccaact ccacaagata ctactggtgg ttcagatagt ggtggaaatt
     7261 ctcaaaactc aaataatcaa taagtataaa atatatgcat aaatttaatg aacaaattat
     7321 atattataaa aaatatctct tagcaatttt ttcttactaa gagatatttt ttgagaaata
     7381 atttaataaa tagcagttaa attttaaact aaaaattaaa gtcacagtag agataagcta
     7441 ttgtaaacga ttatctatat atttatcaac ataagttttt acaacagcag ctattggtac
     7501 tgaaagaaaa actcctatga aaccaaatag agattgtccc accaaaatag cagatattac
     7561 ccatatagct cctataccaa cttgattacc cataactttt ggttctataa aatttccatc
     7621 taattgttgc aatataaata gaaatgcaat aacccatata gttttttgtg gactatataa
     7681 tatagtcatg gctattggag ggattgcacc aataaaagga ccaaaatagg gaattatatt
     7741 acctaagaat attattgaag ctaaaaataa agcattttct attctgataa caaattgaaa
     7801 tcctaaaaat gcaattatac caataataag agagtctaat attctaccaa gtaaataatc
     7861 atagaaaata tcatgagtag tcctaaaaaa ttctataatg ttatctgcct tgtttctagc
     7921 agtagaagaa tatagaaatc tcttaaatcc caatgcaatt ttttctttgt catatataat
     7981 gtatatagat agtataaatc ccattgtcat attaaatagc atagaagata tactaagtgt
     8041 atagattaag aagtttttag gaattttacc aacaaaatta cttgcttctt ttaataaatt
     8101 atctaaacta tgttgtatat gaggaattaa agtttttaga aaatctatgt ttgacatatt
     8161 tttttctata aaactatcta tataatcagt atacataggc atttcattta tcaatctatt
     8221 taatgtatta gctattgatg gaattaataa cctaatggca aaggctaaaa taaaacctat
     8281 aaatatataa gataaaaaaa tggctaaaag tctatgtgct ttgaatttta cctcaaaaag
     8341 catcacaagt ggatttaaaa gtaaagcaag taaaatagct aataaaaatg ggctaaaaaa
     8401 ctttaagaat ccttcaatac tagatataaa gtctgatgga gtattaataa atttaaaaaa
     8461 taaaattcca ataaacacga ctattaaata gtattttata ttttcccttg aaataataat
     8521 cacttcctta aaatagtgtt tagtcagcta ttacatatta atagtatgtg aaaagaaatt
     8581 acaattattt aggaaaatta aataattttt ttgcttgttt ttaattaagg tctagtatag
     8641 tataataatg tagcaaaatt aacattgata agaatattaa acacacataa tttaagataa
     8701 ggaaggatta agtatgagaa ttaataagta tatagcttct tgtggaatag catcaagaag
     8761 aaaagcagaa gaaataattc ttggaggaag aataaaggta aatggaagtg tagtaaaaga
     8821 gttatctttt aatattgatg aagaaaaaga catagtagaa tttgataata aaaaagtaaa
     8881 accaagtgaa aattatatat atatagtatt aaataaacca gagggatata taactacagt
     8941 aaaagaccaa ttcaatagac ccagtgtaat agacatactt aaagatgtaa gggaaagagt
     9001 atatccaata gggagattag attatgaaac tagtggatta ttaatactta ctaatgatgg
     9061 cgatttaaca tataagctta cccatccaaa gcatgaaatt gataaaacgt atgtggcaag
     9121 tgtgaaggga attataagtg atgatgagat aagacaattc gaaacaggac taaaaataga
     9181 agattacaca acggcacctg ccaaaataag ggtaactaaa gaaaataaag aaaaaaatta
     9241 ttcagtgtgt gaaataacta tacatgaggg tagaaataga caagtaagaa aaatgtgcaa
     9301 agctataaat catccagttt taaatttaag aaggatttct gtggggaaaa tagtattaaa
     9361 agacaccaaa gttggagaat atagatacct aacagaagat gaaataaaat atttaaaatc
     9421 aataaaataa gataaaaata aattgagaaa aaatgaaata aagttaatag tagggagaaa
     9481 tagtattatg gcatatgcaa agtatttaac taaaattaca gatataaata aattttattt
     9541 agaaaatatt ataaatgaag gtgacatagt aattgatgct actatgggaa atgggtatga
     9601 tacaaggtat ctagctgaga aggttggaga aaagggcttt gtatatgctt ttgatataca
     9661 agaagaagca ataaagtcta caagaaagaa actagagaaa gaaggatata cagatagagt
     9721 aaaattgata tttgatgggc atgaaaaaat gaatcaatat ataaaagaag aagtttcatg
     9781 tgttttattt aatttgggtt atcttccaag agcgaaacat tcaattataa ccaaaccaga
     9841 tacaacttta aaggctataa agttaagttt agaaatgcta aaagaaaatg gagtaataag
     9901 cattgccata tatacagggc atgatggagg tcaagaagaa aaagattgca tatataacct
     9961 tgtcaataat ttaaatcaag atgaatttaa tgttttagaa tctaagtttt taaatcaagt
    10021 caataaccca ccacaattga ttttaattga aaagaaaaaa gcataataaa tactaaaaaa
    10081 cttgacaaga ttgtattaag agttaatata tatagtgtga ccta
//
LOCUS       DAEMIK010000149         9864 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEMIK010000149 DAEMIK010000000
VERSION     DAEMIK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.151"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            290..2377
                     /locus_tag="KPK87_003174"
     CDS             290..2377
                     /locus_tag="KPK87_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2539296.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNGHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            2392..2679
                     /locus_tag="KPK87_003175"
     CDS             2392..2679
                     /locus_tag="KPK87_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2539297.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2706..3977
                     /locus_tag="KPK87_003176"
     CDS             2706..3977
                     /locus_tag="KPK87_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG2539298.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            4035..4634
                     /locus_tag="KPK87_003177"
     CDS             4035..4634
                     /locus_tag="KPK87_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2539299.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4639..5544
                     /gene="dapA"
                     /locus_tag="KPK87_003178"
     CDS             4639..5544
                     /gene="dapA"
                     /locus_tag="KPK87_003178"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2539300.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5650..6282
                     /locus_tag="KPK87_003179"
     CDS             5650..6282
                     /locus_tag="KPK87_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG2539301.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            6760..9267
                     /locus_tag="KPK87_003180"
     CDS             6760..9267
                     /locus_tag="KPK87_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2539302.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            9609..>9864
                     /locus_tag="KPK87_003181"
     CDS             9609..>9864
                     /locus_tag="KPK87_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539303.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTT"
BASE COUNT         3745 a         1102 c         1769 g         3248 t
ORIGIN      
        1 atgtgctttt taattgtagt aatttattta taatcaatat gatttttatt atttaaatca
       61 atttttcttg agtttctttg tctaaagaaa ctttttttat ttataaaatc tacagttata
      121 ttatagtatc tattttagag aagtttaatt tttgtatcaa tctaaaacaa aagttatact
      181 taaatgaaat tgaaaacgat aaaattctca aataaaataa gtattgtgga ggtgatgaaa
      241 gaatccaatg ataatatgaa gactaatctt aatgagaaag taggggttaa tggtagtgga
      301 caaagaaatt gtatcaattc tccagatttt attaaatagc acattgatta caactaatgg
      361 attacaagag gagtctaatt catcaaagag acaaattact tatagaataa ataaaatcaa
      421 tgacatgctt aaagtaaaaa atgtaccact tatatgttta agagctgata aagatattat
      481 cgttagaaaa gaaacaaaaa aagcaattaa agaaatttta gaaaaaaact attctaagaa
      541 tacttattat tttagtaaga cagaaagact tttatatatg tatttaatgc tttttattaa
      601 catggatgat atttcaatta atcattttat aaattctata aaagtaagta ggtctactgt
      661 aaatcttgat ttcaaggatt tgataccaga gcttgaaaag aagaacatta aagttaagaa
      721 caatcgtata aatggatatt acatagttgg caatgagatg gaaattagga gagtgcttat
      781 taaaaacatt atagaaactt tgtctaatga gaaaagttca agagtatttg atatttttat
      841 taaagaatat aatttagata gttttgaaga ggctaaaaaa attatattaa agctaataaa
      901 aaaatatgaa attacttttg ttgaagatag attaatagaa tttatatata tatttatctt
      961 tctaaaggca aggatgtatt caactagaat agttactcag gaaaatgtag atattcctaa
     1021 tatttcagtt atgagttcaa tgaaggagta taaatttgct aaggaattat tagaagtata
     1081 tgggagtgaa gataaaataa agcctcacaa tataatgtat attagtgctt ggattttagg
     1141 tatatcagta ggaaattatg aagaagatac agaagataag gatgtaattt caaaaattgt
     1201 aaataaaatg atgacaaagt ttggatattt aagcggagtt tactatataa gccaagaaaa
     1261 aatatttaaa caattatatt ctcattttag acctgcctac tatagacttt tatttaaatt
     1321 acctatttat aatccgattt gtgaaaaagt aaaagaagaa tataaattag tttatagaat
     1381 tgtaagtaat gcaatgaaag agttttgcgg attatttgga gaagaaattc aggaagaaga
     1441 gttagcatat cttacaatgc actttgcaac acttttttca aataaaaaag aatttgatgg
     1501 ttttagaaaa agaatagcat taatagtttg ttcaaatggc gttggtagtt cagctatttt
     1561 acatgcagaa cttacaaact tatttcctga cttacacttt ataccaatgt cggaatcagt
     1621 tggattagaa aatataagtg aacctgtaga tattgtattt actactaatt ataacgcaga
     1681 gggtttaaaa acagatgtac cagtaattaa agtaagccct gttatgacca caaaagaaaa
     1741 atataagatt acacgtgaag tgtatattca attaggtgat atttttttaa gacaacctaa
     1801 aatagatgag gttatggata taataaaaaa atatgcaaat gtaacctctg aaagtattct
     1861 ttcaagtgag ctattagctt attttacaca gatagagaat tttacatcta aggaaggaga
     1921 ggggcctatg ctaagtgaga ttactaatga aacactagtg aaaataaagg taaaagctaa
     1981 gaattgggag gaagctatta gacagtcagc ttctgtatta gttgaaaata acaaagtaac
     2041 tgaagggtat gtagatgcta tgattaactc agcaaaagca tctggaccat acatagtaat
     2101 tacaaaacat gttgcacttc cacatgctag accagaagct gggtcaaaag agattgcaat
     2161 aggcattgct actttagata gtcctattga gtttgggaat aacgaaaacg accctgtaaa
     2221 atatgtattt tgtctaagtg caatagacaa taatggccat ttacgtgcta tgtctgagct
     2281 agttgaacta ttggaagaag atgagttttt taaggtttta gatagtgcta aagaagctaa
     2341 agaaattatt gattttataa aaacacacga attataaagg aggatttaat tatgtataaa
     2401 attttaatcg catgtagagc aggagttggc tcaagtttaa tgttaaaaat aaagactcaa
     2461 caaattatta aggaaaataa ctttccaatt gaagtagagc atggttcatt ggattcacta
     2521 aatggattca atggagatgc tgtagaaaca ttaattgatg ttgctgaaga attaaaaaat
     2581 aaaaatttga agtttgatat aattgggatt cacaatattg ttgaccgtaa tgaaataaag
     2641 acagcactag aaaaattcct tgcttcaaaa gagggataaa tttaagatga taaggaggaa
     2701 tatttatgga gtttattatt agtttgctga gtacacctgc tgttttgctt ggcgtagttg
     2761 catgtatagg attgattctt caaaagaaat cagctgttga agtatttaca ggctcttcaa
     2821 aaacacttat cggttttttg atatttggta ttggtgcctc tgctatgaca ggagcacttc
     2881 aaaatttcaa taaactgttc caacatggct ttggaattac tggtgttata gcttcaccag
     2941 aagcagctac agcacttgca caaggtacat atggttttgc agtttcatgt acattaattc
     3001 ttggattcat attaaattta gtatttgcac gtattacaaa gtttaagaat attttcttta
     3061 caacaggtca tagtttgttc ttttcttgtg tattagtttt aattatgaaa gcacatggat
     3121 tcgataatac aatcactatt ttaataggtg gaactatctt agggtttatg tctgctgcac
     3181 tacctcaatt ctgtcaacca tttatgcgtg aattaacagg tggcgacgaa caagctattg
     3241 gtcactttaa catgattggt tatggtttat caggttatat tgggcgttta ttttcaaaac
     3301 ataaagatga tacaacagag actatagaat ttcctaagtg gttatcaata ttcagagact
     3361 tcttaatggg tctatccata gttatgttaa tcttatttta tattgcaaca ttaaaagctg
     3421 gtcaagcatt tactcaagaa attgctggtt caacacattg gttggttttt ccgcttatag
     3481 aggcatttac atttgtggca ggtatgtcaa tattgatgtc aggtgttcgt atgttcttag
     3541 ctgaaataac tgcagcattt gtaatcattt cagaaaagta tattccaggt tcacgtcctg
     3601 cgctagatgt tccaaccgta tttccatatg caccaaatgc agttattatt gggtttatat
     3661 cagcatatgc tgctggtcta ttagcaattg ctattatggc atcatttcct agtatatttc
     3721 ctgtagtaat tattccagct gctcacatat gttttttctc aggaggtaca gctgcaatat
     3781 ttggcaatac ttcaggagga tggagagggg ctattgctgg gtcatttgtt gtggggttat
     3841 tacttgcatt tttgccagtt atactatatc cagtttatgg gactcttggt atagaaggag
     3901 caactttccc taatattgat tacaacgtag ttggttcgat attacataat attttacaat
     3961 ttataaaacc tatttagact aaattatata aaagtatagt catagttact atgatttcaa
     4021 ggaggataca tcttatggaa aatacattat ctgaattttt agataatata tctaatgaat
     4081 tgtcagaatt tattgcctca atagatgaga catctataaa taaagcatgc gaattaattc
     4141 ttgaagctga aaaaaatcac ggacgtgtac atgtgactgg tattggcaaa ccaggacatg
     4201 tatcgggata tatttcatca ttgctttcat ctactggaac gtcagcatat atattgcatg
     4261 gtacagaagc agtacatggg tcaagtggtc aagtagttga aggagatgtt gttattgcaa
     4321 tatcaaatag tggtgaaact caagagttaa aagcaactct taagactcta aaagtaaatg
     4381 gagcaaagat aattggtgta agtggtaatg agagttcatt tttaaaaaat atttctgata
     4441 tattcctgtt tgcaggtgtt aaacaggaag gagattgtct taataaagct cctcgtgctt
     4501 caattttagc tgaaacaata atattacaat cattaagtgt tgtattacaa tatgctaaag
     4561 gtcttaatac acagcagtat ttgaagtggc atccagcagg aagtcttgga aagtctatta
     4621 gagagggaat ataattttat gaaatataaa gggattatat gtgcaatgat aacaccattt
     4681 gatgaaaatc aaaatattaa ttcacaagca acatgtcaat taattgatta tttgattgaa
     4741 aagggaattt atggattatt tattttgggc actaatggtg aatgtcatgt attaacagat
     4801 gatgaaaaag ttgaatttgc aaagattgtt attaatcata caaataatcg tgtacctgtt
     4861 tttgttggaa cagggggtaa ttcaactcgt gaagttgtag atttatctaa gagaatggaa
     4921 gcgattggag caagtgcatt atctattatt acaccatact ttgttacacc aacacaacag
     4981 gaattaatct tacattacaa aactattgct aattcagtta atttacctat tataatgtat
     5041 aatatgccag gtaagactgg aattaatatt gaaccagagt cagtacgtga attatcaaag
     5101 ataaagaata ttgttggaat taaggatagt agtggtaaac ttgacaacat gaaagcttat
     5161 attgaagcaa cgaagaatga gaattttagt gtgttttcag gctcagattc acttattctg
     5221 gatagtctaa aagctggtag tcaaggagct gttgcagcaa caacaaactt cctcacagag
     5281 attggtatct ctatctatga aaactttatg aaaggggatt tggaagaagc tcaaaaagct
     5341 caaagtagta tagaagaact tcgacgtata ctgaaacttg gaacaattcc atctgttatg
     5401 aagaaaacta ttgtcttgaa tggaattaat gttggaactg cacgacttcc tgtaactgaa
     5461 ccaactgggg aagtacttga agaaattaaa cgagtagttg aaaattatag gacaatactt
     5521 aataaaagta gtgagaatag ataatataaa tagggaaatt acactggtat ataattaata
     5581 tatatattat aaaacatgac taaaattaat gaataagtaa agtggtttga tgagtataga
     5641 ggtgataata tgaaaaagat gaatataacc aaaagaatgt cagaatgtgg ggctcttgca
     5701 attgtaagag aagaaaatct taaccgtgca tgtgaaattg ctgaaggatg tataaaaggt
     5761 ggtataacag ttatagaaat gagttatacc ctaaataatg ctggagaaat aattcaaggg
     5821 cttaataaaa agtatggtga aacactttgt gtgggagcag gaacagtttt ggatagtgaa
     5881 acagctcgtc atgctatatt acatggagct caatttatta ttgcaccaaa ttacaatgaa
     5941 ggtgtagcga agatttgcaa tagatatcag attccttatg ctccaggatg tacatcattg
     6001 actgaggcag tagacgcact aagtttagga gcaactttta taaaagcttt cccaatatca
     6061 gatttctatg gacctaaact agtaaaggta tttaagacac caatacctga tatgccagtt
     6121 ttagcaagtg gagggattaa tattgaaaac ctacatatat ggttagaaaa tggagttgat
     6181 gtatgtggat ttggtggttt attaactaaa ggttctgttg aagatattgc taaaaatgct
     6241 aaaaagattc gtgaaatcat taaaaatata agaaaaattt aacatagggc tgtatcatag
     6301 tatcttataa ttactatgat acagcctttt taatattatt caaatttaaa acagtttaca
     6361 tttctattat attacatata atttcagtaa ttttatctta atttttctat ctggaaaaaa
     6421 atgatttaaa acttaagtta tgatataatt tcattaaaag aaggcgattt ttagtaaata
     6481 tgagaaaata atgtgttttt aggggaaaag ttaaatacag atttaagtga acaagaatcg
     6541 ataattaaga tattaaatgg tgaatagggc atcaaaaaaa ttactaaatt aaaagtttta
     6601 gagggagaat cttagcaagt gtgtgtgaaa attcaatttc aaacaaaaaa taattgtaaa
     6661 tgaaatcaat ttatacacgt tgaagattca cttttatcat tatattaaaa agcagagtga
     6721 attaaaagtg agtctaaaga taggaggata attaaaacaa tgaaagaaga cattaggtta
     6781 catcgaatgt gtaaaaaaga acaagcaaca aagaatgaag ttatatcaga ggatattcta
     6841 aaatctatga gtattggggt cagtaaacat tttttagatg attcatttac aataatatgg
     6901 ggaaatacat cattctatca attattagga tatacagaag aagaattttt agttaaattt
     6961 tctagtttaa aagattatta tgaacagaat ctttatcatt ttgaatctat gaaaaatcat
     7021 tttttagagg cttatcatca tggcgaaaag agtatagaat atcaaatttg catttcagca
     7081 aataatggag accctgtttg ggtaataatg actggtatat taattgaatc acaagataat
     7141 gagaaatcaa tggtctatat attgtattca aaaattaatg agttaaaaat aaagcaagaa
     7201 gaactatctg atatggaaaa agagagtaca gagaatttta aatggatgat gtcagagtat
     7261 gctgggaatg tttatattag cgatatggat acatatgaat tactatactt aaataagcac
     7321 tcatgcaata ctttacaggc atcagcaaat gaacttattg gaagaaaatg ttatgaggta
     7381 attcaaggta gaacttcgcc atgtccgttt tgtacaaatt cgtatttaaa aaaagatgac
     7441 atatatgaat gggaatttta taataaaaaa ctgaaacgaa catttataat taaagaccgt
     7501 atgttgaatt ggcaagggcg aagagttcgt attgaattat cttatgatat gtatagtgct
     7561 gaatataaat tagcaaaaaa agatcaggaa agagaatcaa ttttgaaaac tattccagca
     7621 ggaatggtta gattagatgc aagagattat cttactatac tttggtgtaa tgatatattt
     7681 ttgaatatga ttgaatatac aaaggaacag tttgcagagg aacttaataa tcaatgtgga
     7741 tatttatttt ctgaagatta taaacgtagt aagaaactgg cacaagattt gaaggagtct
     7801 ggggataatg ttgtatttga agcaaaaata tatacacgtt caagggaaga acgtatatgg
     7861 actgttacat tgtgttatat cagtggggaa gacagttggg atggaatacc atctttttat
     7921 agtataggta ttgacataac aaaggaaaga aaacagattg agaaattaca gcatatatca
     7981 gagaaagatg cattaacagg aatttataat cgtgctgaga ctgaaaggca gattaagaaa
     8041 tattttgaaa aaaatttaaa tgttatgggt gcactgttta tgattgatac agataatttt
     8101 aaacagatta atgatacaga agggcatatg attggagata tagtacttac tgagatggca
     8161 tcaggtatga aaaaaataat gcgtgacagt gatgttgttg gaagaatagg tggagatgag
     8221 tttacaattt ttatgaagaa tatttcctca gttaaagatg cagaaaagaa agcagaagaa
     8281 ctattgtata tgttccgtca tctatttcag aaagaaaaaa gttttctaaa agtaacttgt
     8341 agcataggaa tagctatata tccaaaggat ggtactacat tcaaggaaat atatgcaaga
     8401 gctgataaag ccctgtatca agcaaaaaat atgggaaaaa ataattatgt aatctacaac
     8461 caagattatt ttaaagaatt agaagaattt gattgttctt ctcttggtac agttattgat
     8521 tcagaaaaac aatacgctga atatcctgat aatcttacac gttatatatt ccgtatgctt
     8581 tatcagactg atgatataga ccaggcgatt aatatgatac ttgaggttgt tgggaaacag
     8641 tttgatgtaa gtcgtgttta tatttttgaa aatacagaag atggtagata tacttctaat
     8701 acttatgaat ggtgtaatga aggaatctct tctcaaatgg cttatttgca aaacagcaat
     8761 tatcaagact atgaggatta tgaaaagctt tttgaggatg aattagtttt ttattgtcga
     8821 gatatccata cattaagtcc taaactagaa gaattgtttt ctagtcaagg tatccactct
     8881 acacttcaat gtgcatataa agaagataga gttttcagtg gatttgttgg atttgatgaa
     8941 tgcacaggac tacgtctatg gtcacaagag gaaataagta cattatcatt gatttctcaa
     9001 attatatcaa tgtttttaca acgtaaaaaa aataataaac ttaatcaaca gattcaacaa
     9061 taccagaata ttttagataa tattgaccag tgtatttgtg tgattaataa agaaaataat
     9121 tttttattat atgtaaataa aaaatttaaa aatacatatt ctaaattcca aattggacaa
     9181 ccttattatc ttgattctat agatattcat aagatatcca ttatatggaa taaaaaagaa
     9241 gcatacctat gtactagcac gatttaatgg taaagtaaag tttatattgg acttaaatta
     9301 aatttcgttt taaataagat attattttag ttgcttactg aaaagactag aatctatatt
     9361 aatgaattat actaatttta taccactatt aaaatattgt ttgctaaaaa taaaaaatag
     9421 ccttaaaaca gttgattata aaaaattaac tgctttaagg ctatttttat ctttgtgacg
     9481 aaatagacac ttgccattta gaatgtgaca atatagtagt tgtttagaaa aggcacaaag
     9541 cattatactt taactaaagt aaaatgtatt ttgcttttag aataaaaaca attaggaggg
     9601 agctataatt gaaaaagata atatatgtat tatttacaat tgaactatta atagtatctt
     9661 tattaggtct aaatatagtt aaagataatg aaattaataa tattttatat aacgatacaa
     9721 caagtatatc tgtaatgttt aaagattata aaaagttaaa taaaaattat agtaagtgga
     9781 ttaaagatat tgcagatgaa aataatgtgg ctatatcaaa gtatgttttt aataataatg
     9841 aaaaattatc tatatatacg actg
//
LOCUS       DAEMIK010000150         9853 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEMIK010000150 DAEMIK010000000
VERSION     DAEMIK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.152"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(208..1785)
                     /locus_tag="KPK87_003182"
     CDS             complement(208..1785)
                     /locus_tag="KPK87_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG2539304.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGAAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNEDQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="KPK87_003183"
     CDS             complement(2082..4493)
                     /locus_tag="KPK87_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG2539305.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4720..5115
                     /locus_tag="KPK87_003184"
     CDS             4720..5115
                     /locus_tag="KPK87_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG2539306.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFGSKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFRK"
     gene            complement(5160..6986)
                     /locus_tag="KPK87_003185"
     CDS             complement(5160..6986)
                     /locus_tag="KPK87_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2539307.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWTKSESAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALNSEIEKQLNAENINFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIAKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTHIKNIYVTKDGTKNKNDLIDSLAVGV
                     LAAKNSSPILLAGNKLDTTQKNVLNTKIIDSVIQVGGLGNEESVKNIVDMQEKTKYIV
                     ETVDELYVAVKKADANDVIEFKPKKEVSDSIDIETSKAISINFEGVYNQSITIDMPNS
                     EIKNIGKIKDTLQIKNIKSSTLINEGTIDDLDIYDENGCRIENESSGEISLITILKDA
                     KNVYIINEGNITKILNNCNDVVVRNSGKIDIVSGSEQPAISGKKPNINDTEDEEELAR
                     GLNVKSEACSSPRKNYVDITILSNLTSSKYELYYRIVNYKPSARYVGQQINTSDWEIV
                     PVYGSKFTKRVKNGSYIEVVEVKNSTNEISRWGRSNITDDGF"
     gene            complement(7019..7726)
                     /locus_tag="KPK87_003186"
     CDS             complement(7019..7726)
                     /locus_tag="KPK87_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2539308.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     VRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            complement(7833..9704)
                     /locus_tag="KPK87_003187"
     CDS             complement(7833..9704)
                     /locus_tag="KPK87_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG2539309.1"
                     /translation="MNRKNLSVIMATAMISTSVAPVFAAETTHVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKIGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKSKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYLGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKAIELTVESNNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIIIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNIERLAGDDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
BASE COUNT         2864 a         1768 c         1135 g         4086 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 acacacttca cttaaatata catattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatgatt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tatctctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagttgatc ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctata actttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaaggctc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatctctta taaatttatc
     1201 aaatgcttct acgccatctt taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgctcca acactaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagcacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatgt aaaaatatag acaagcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc ctttttgtta ttttatattt ataaacttct attgaacatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
     2221 ggtgaatcag ttttagcacc taatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg atagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta cctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc gattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcgtctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaatttatt attttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatatcagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcact atctggtttt gatactgatt tcattgcata gttatctgta
     3541 tctgttagta gagttttatc ttcataagaa atccagaaga atcctttcat agaattaaac
     3601 tctccccaac tgctttttac taaccatgct ccatttgatt ctggttttac atcagatgca
     3661 aaattatctt ttgaataatt atcatcccat cctactatcg ctacagcgtg atttaatgga
     3721 gctctcttat ttgtacagtt atatgctgtt tcatctttat taaaatatgt tgaataatgt
     3781 gcataacctg atgtcactga accatattgc attatagcat tctttacagt ttccttatcc
     3841 ttattaagac gaacaacatc tgttacatta aattgagtag gtgcagtatc catatttgaa
     3901 ggtttagttg caccttgtgc ctcagattta agattatatg gtatgtcctt ttctaactta
     3961 ggacctgccc atgctgtcag atatcctata gctgttacat ttgaactccc tgacatatca
     4021 tctaagttcc atccatattt tccaccagtt gcccaccatc ttaaatgttc ttctgataag
     4081 tcatacgttc catatccttt taatttaaga tatgcttcta acgttgacat acctgaaaaa
     4141 gaccagcatg tattaagact tccttgattt tttgctggtg tagtaagacc aagatccatt
     4201 gggttgtatg ctacacttga tttatatgct gatggaagac tacttctacc cttatttcga
     4261 agtgttgtac cttccatttc atgtggaaaa ggtattactc cattatattt tgctgtgtct
     4321 ccatttttga catcttctag gtattgaaga tagctttcag aatactctgc agtttctact
     4381 ccatctagag ttttatggtt ttctgctgag acaggtattg ttgacactat taaaaagcaa
     4441 actgtcaaca atgctagtag ctttgacaat ttttttgatt tgtactttct catgtttacc
     4501 cccttatatg tatttaattt ttattatatc agatttctaa attaaggtca tattttattt
     4561 tgataaaata tggattatga gtacatatta attatagaat attatagttt tatttttata
     4621 atattttaag atatattgat ttattaatag atgtgctata ctaatgtttg ttagtatctt
     4681 taagtatatt tatattaaat tagcttagga ggattatatt tgattttaaa aaaacataga
     4741 gagacaatat tatatttatt ttttggagca tttactactc ttgtaaatat agtttcgtat
     4801 ttatttttta ctagagttat tttatttaat tttatggttg caaatgcttt ggcatggata
     4861 cttgctgttt tatttgctta tgttactaat aagttttttg tgtttgggag taaaagaatt
     4921 gaaataaagt tcttattcaa ggaatttttg tcttttgtta gttttaggct actttctggt
     4981 gtcgttgaaa tgttaattat gtatgtaatg attgatttgc tttttgtaaa tgatgttatt
     5041 gtaaaaatat ttactaatat agttgttata gttttaaatt atttgtttag taaaatgata
     5101 atttttagaa aataaatagc agacaataaa aaaacagttg tctgcttaaa ataatttatc
     5161 taaaacccat catctgttat attagatcta ccccatctag atatctcatt tgttgaattt
     5221 ttaacttcta caacttctat atatgaacca ttttttactc ttttagtaaa cttacttcca
     5281 tatacaggaa caatctccca atcacttgta tttatttgct gtcctacata tcttgcagaa
     5341 ggtttatagt taacaattct ataataaagt tcatacttag aacttgttag attgcttaat
     5401 atcgttatat ctacataatt ttttcttgga gaactacaag cttctgactt aacatttaat
     5461 cctcttgcca actcttcttc atcttcagtg tcattaatat ttggcttttt accacttata
     5521 gctggttgtt cactcccaga tactatatca attttacctg aatttctaac tacaacatca
     5581 ttgcaattat taagtatctt agttatattt ccttcattta ttatatatac attttttgca
     5641 tccttcaata tagttataag tgaaatttca ccagaacttt cattttctat tctacatcca
     5701 ttttcatcat aaatatctaa atcatctata gtaccttcat ttattagagt actacttttt
     5761 atatttttta tttgtagggt atcctttatt ttaccaatat tctttatttc agagtttggc
     5821 atatctatag taattgattg attatatact ccttcaaagt taatagatat tgctttacta
     5881 gtttctatat caatagaatc acttacttcc tttttaggtt taaattctat aacatcatta
     5941 gcatcagctt tttttacagc tacataaagt tcatccacag tttctacaat atacttagtt
     6001 ttttcttgca tatcaactat attttttaca gactcttcat ttcctaatcc tccaacttga
     6061 ataacactat ctataatttt agtatttaat acattctttt gagtagtatc aagcttattt
     6121 cctgctaaaa gtattggaga actattttta gctgctaata cacctactgc taaagaatct
     6181 attaaatcat tcttattctt tgtaccatcc tttgttacat aaatattttt tatatgagta
     6241 tctttataaa attcttctat aatctttgca tttgtctcac ttctactact acctgctatt
     6301 cttgttgcat ttggtaaact tctttctaca gaattagaaa tagaatatgt accacctatt
     6361 acatacgatt ttgctatatc tttactatct ataaaattat cagctacttc agttccattc
     6421 tctgaatcag aaagtattat tggcatgttt tcttgagcag ctatagctcc aacactcact
     6481 gcatcagcaa gtcccttttc tccatttact actactattt tagatataga tttttcccta
     6541 tctagctttt cagctaattt taaagaagta tcatatctac tatttccgga tattctttca
     6601 aaatttatat tttctgcatt caactgcttt tcaatctctg aatttaatgc aattgaacca
     6661 cctattaaat atacattttt cacaccaagt ctttttaatt ctgcttttgt tctactatcc
     6721 aatttattac tttgagttaa taatattggc gcatcttttg cctttgcaaa tggagtagct
     6781 gataaagcat ctgctatgga attgtcattt actaagacag cactttcaga ctttgtccac
     6841 ccagcttgac ttatttttat tgctgtttcc catcttccag aacccgttaa tgtatctttt
     6901 gtagatgcat tagatgtatt tccatataat gtaacagtta aaaatgtcat agttaacaaa
     6961 gacactatct tttttgatat tttcattaat tcttacctcc taattctttt ataatatttt
     7021 atggagcatg tacatattgt attttattta tagctagctc atcaaaatga gattttactg
     7081 aatgatatcc tatagcataa tgtccactct caggattcca tttcttatat gcattcattg
     7141 cacttaataa cttctcctct tgactaggta ataccttcca tacctctaca tttggattaa
     7201 cttttttaaa atccttatag tcatatggtt ctacataaaa tcttaaatct tttattttgc
     7261 cttctctata caattctagt gcagcttctc ctaatgctct atggtcttca tgtattccac
     7321 tagctttaaa tggagtaaca gtttttactt ttgcatctgg atattcttct aagtacttaa
     7381 gtattatttc ccttgccttt tccttactta gctcaccatc atgtgctcta ttatcttcta
     7441 tatgtatatt actttctttt acacccattg cttcacagct atccttaaat tcttcatctc
     7501 tatcttttga aaattcactt tctgataaag aatatttatg aacatcttta atatgatacg
     7561 aacatgaccc tccatcatcc aaaacttttc ttactattga tttactacca tctgtgcata
     7621 gtatgacatg tgtatcactt ttttcaacat gttcaattat agcacttccc atacttaagg
     7681 tttcatcatc ttgatgtggt gtaaaaaata aagttgcatc taccactact acatcctcct
     7741 actttataaa atgttataat ttacaataaa tagcataatt atagaaaaag attcctgtat
     7801 taattaagga atcttctcca cagtataagt tattaccaac ctagtatttt agctatcttt
     7861 tgtattactg tcaattcaac tccattacct atttgagtta cttctgcacc aagatttaat
     7921 tttttactaa gtgcattctt ttgtgagtct gttatatcag ttttagctag aagtattgga
     7981 cctttacctg ctgctagagg tcctgctgcc aatgcatcta ctagcatacc attatttcca
     8041 tatccatctt ttgctatata tagtttatct aatttgccat aatatgtttc tataacttta
     8101 gcattagtat cgtgtcttgt ttctccttca actctttgaa cttttctatc tttatcaata
     8161 tcggcaagtt gattttcaat ttggctagat acattgttag agccaccaac tataccaatc
     8221 tcttttccat ccatcaattt gatagcgtct gctgaaagat cattccagcc atttactatt
     8281 ataggagcct taagttcagc agctttagca gctatactca tagcatcagc ctcccctttc
     8341 gcacctacta caaatgcatc tttaaaagaa cccatagctt ttgctactgc tacagaagtc
     8401 gtatgtctat catctccagc tagtctttca atattcttag ttactgattc taattgcttt
     8461 ttagctgtat ttgatactgc tgattctcca cccactatat atattttagc tgatgggctt
     8521 ttcttaatta tatcttttat ataatctaaa gttacttttg gtatttcatt atcagatgct
     8581 aacaatatag gtgctttttt agattgagca agtggtgtag ctgctaatcc atcaactagt
     8641 ttatttgaat taactataat tatgttgtca gtatattttg tttgttttgc tatagccact
     8701 gcagttgcat atctatcatc tccagctatc aatttaacct ttgaattacc ttctaatgca
     8761 gctttggcat cttttaaatc tttttcatta ttactctcta cagtaagctc tattgctttt
     8821 ccagatgcat cagtatactt aatagtaaat ttatattttc ctgtttctga atcttttgct
     8881 gaaatatcac taattgactt tgatgtacca tttgctacca tattaccttt aacagttaat
     8941 ctatatccat catataaatc ttgtggtgaa actgtattta ggtcactatt ttttatagtt
     9001 atttcggcca attggacatc atcaatattt ttagctcctg ctacacctag tttatcgatt
     9061 ttatttactc taaatccttc tgcagaaggg tctaagttcg ttccagttga gtcaactgga
     9121 tttgctgtta aatcaacttt ttcatcacca atacctaaat atatagtttt agaagttact
     9181 gtttctgttt tatctcttaa agttataact aacttatctt tagcactatc atatgaagct
     9241 tttacatctg caactttata aattccatta gtttcagttt tagatttttc tgttatagag
     9301 ttagcttcag cggaaaggtc tgctgaattt tcatattttt cagttgcttc tgcaactact
     9361 tcatttgcac tatttgttat atgcccttta tctgttatag ttacaataag ttctttattt
     9421 tctcccaaag catttactaa tttctctaat tcatctatat tagttattat ttttaatttt
     9481 gataaagtct caccaatttt tatttcatat attggttgtc caacttgaga acctcctgta
     9541 tacttttgag acattaaatc tctaactttc gaaactagtt ctgtagcttc tttcttagtt
     9601 attgtttctt tttttacatg tgtagtttct gcagcaaaaa ctggagctac tgatgtactt
     9661 atcattgcag tagccataat tacagaaaga ttttttctat tcatggtatt ttccccctaa
     9721 aaaaattcaa atttttatta ttttttcctt atttaccaat tataatatat atttgatatt
     9781 atttcaataa actagatcta cgtaatattt aactatttta caaaactaat cttatgaaag
     9841 tatattttat tta
//
LOCUS       DAEMIK010000151         9787 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEMIK010000151 DAEMIK010000000
VERSION     DAEMIK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.153"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            215..679
                     /locus_tag="KPK87_003188"
     CDS             215..679
                     /locus_tag="KPK87_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBG2539310.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNIWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            1316..2332
                     /gene="gap"
                     /locus_tag="KPK87_003189"
     CDS             1316..2332
                     /gene="gap"
                     /locus_tag="KPK87_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG2539311.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            2714..3883
                     /locus_tag="KPK87_003190"
     CDS             2714..3883
                     /locus_tag="KPK87_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539312.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            4264..8517
                     /locus_tag="KPK87_003191"
     CDS             4264..8517
                     /locus_tag="KPK87_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG2539313.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            8666..9160
                     /locus_tag="KPK87_003192"
     CDS             8666..9160
                     /locus_tag="KPK87_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG2539314.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            9276..9653
                     /locus_tag="KPK87_003193"
     CDS             9276..9653
                     /locus_tag="KPK87_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG2539315.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         3959 a         1005 c         1761 g         3062 t
ORIGIN      
        1 aataagtgac ttgactctta aattttcata atatgttata aaccaattta agatactaca
       61 ataattaaaa ttccaaaatt atttaacata tttgcaatta aaatgagaaa aggtaaaggc
      121 tgaatgattg aaaaatttga tgtaaaatag tagattagtt taatactata gtttgtaggg
      181 ttaaaaatta gaaattgtaa gggagataaa ttctatgaaa aaattagtta ttattttaat
      241 tagtatcatt agtgtggtta gcttgacagc ttgtaaaaaa agcaatgaaa gtaaatataa
      301 taatttaaac aacaaagaat caggacaaac aaccattgaa atggacttag ataaaaatta
      361 tgatacttca gacccatttg ttaatgcacg tttgttttgt gtgtctaatg atatagatgt
      421 tttagacaca gagatttctt ttaaaatgga tggagatagt ggtattgtgg aaataaagga
      481 caataaaaca gatgaaactt tatggagtaa tatatggcat ggaagcgttg ataatgatac
      541 attcgcaatt tctctttcta atatacaaaa agagaaagag tatgctatat gctttactgg
      601 aattaaaata aatcatgcag ttgtaaaggt tacttttgaa agcaatttag ttaaggagaa
      661 agaaaaaccc tcaaaataaa ttccagattg tctataactt gatattcagt acataaaagc
      721 agttgatatt tatgattgac tgctttttct tatcaaaaag gataggttgt gcatttttac
      781 ttgaaatagt ttatgcacat caaaagttgg tgagcatgat attggaaatg gtaagcatga
      841 aatcttgata agacaaggaa ataaatttaa gttttcaatt aagtatattt ccataattta
      901 gttaataata agctaattat tatttatatt ttgatatata aataataatt gtagcaaatc
      961 tcaaaaaatc atatattttt tgaactaaat aaaaatttat ttttattatt ttaaattaga
     1021 ggagattctt gaagatatat tatttttaaa aataagtata gtttaaaaat tcaaaaaaaa
     1081 gatggcaaaa tatgtcccaa gggacattga gtaaaataaa tgatttcagc ttctttacac
     1141 taaaaagtac atactaaatg aggggtaaaa taaagaaaaa tgatatacta attgtctttt
     1201 gataataaaa aaacatggaa tttgattatt taatcaagaa aaagtattga acaaatgatt
     1261 tatttagtgt acaataatca tgtaaaaaca tagtaatgga gggagacatt tcataatgaa
     1321 aattaaggta ggaatgaacg gatttggaag aataggaaga gctgtattaa gaatagctca
     1381 agaagaatta ggagataata tagaaattgt agccataaat gcaagagcaa caacagaatc
     1441 tttggcacat ttatttacat atgattcatg ctatggaact tttagaggtg aagtagaggc
     1501 aaaggatgaa gacactctag tagtaaactc taaagagata aaaatattaa gatataatga
     1561 cccagaagaa ttaccttgga aagaactggg ggttgatata gtaatagaat caacaggatt
     1621 atttacacaa agagaaaagg cagaaaaaca tattaaagca ggagcaaaaa aagtaattat
     1681 aacagctcca ggaaaaaatg aagatataac tatagttatc ggagttaatg agaaagaata
     1741 tgataatgaa aagcacaata taatttcaaa tgcatcttgt actacaaatt gtctagctcc
     1801 ttttgctaaa gtattggacg aaaaatttgg tatagtaaaa gggttaatga ctacagtaca
     1861 ttcatatact aacgatcaaa gaatattaga taaaagccat aaagatttga gaagagcaag
     1921 agcagcggct gaatctataa tacctaccac tacaggagca gcaaaagctg tgtcaagagt
     1981 actaccacaa ttagaaggaa aattaaatgg tttttcactt agagtaccaa caccaacagt
     2041 ttcacttgtt gatttagtgt gcgaacttaa agaaaacgtt acatctgagc aagttaactc
     2101 tgtgttaaaa gatgctgctg aaggagaatt aaaaggtgtt ttaggatatt gtgacaagcc
     2161 acttgtttct atagattata aaggagattc aagatcatct attatagacg ctctatccac
     2221 aatggttata gaagacaata tggttaaggt agtttcttgg tatgataatg aatggggata
     2281 ttcttcaaga actgttgact tagtaaaata tgtagctgaa aaattaaaat aatatatatt
     2341 tcttaaagca aattttaaaa aaattatata aaactaaaaa aaaagattat gaaaaatatt
     2401 gaaggaaaaa ttttttcata atcttttttc tttcatgcaa aaagatacaa gttacaactt
     2461 atatttgcat attaggtcgt tagagaagta tatatatatt tagtaaggaa tcatactttt
     2521 tataaaatga aagataagat gtcgaaaact tgcataaatc tgaaaaattt ttattattaa
     2581 aatactgatt ttcattttaa aaatgataaa aaaatgacaa ataaagttgc aaatagtaat
     2641 acttataagt ataattaaaa aagagaaaag gttttgttaa actttataac aaaaaaagaa
     2701 gggatgattc aaaatggaga agttaacaga caggagacag gttaaggcag taagcatgga
     2761 aaccttcatt ttcctaatat tactagtagt tggttttggg tatgttggta gtattatggg
     2821 agcaggaatg atgttcaaag taataatgag tacagcacat gctttactat tggagacagt
     2881 tttcttaatt atggctatgg cagttcttgc aggcgcatta agcgcactac tttcagagtt
     2941 tggagtaata gcgttaatta ataaaatttt cgctgtattt atgaagcctt tatatggact
     3001 acctggtgct agtatagcag gagctataac aacttattta tcagataatc cagctattat
     3061 accttttgct aaagataaaa cattcacaca gtattttaaa caataccaag ttccagcatt
     3121 atgtaactta ggaacagcct ttggtatggg acttatctta acaacattta tgatttctca
     3181 aggtacagaa tatgtacttc cagcattaat tggtaattta ggtgcaatta taggtagtgt
     3241 cataagtgtt agaataatgt tgacatttac taagaagttt tataaataca atccggaaga
     3301 agataaagca acaggaacat tagagaaaaa ggatgaattt agagaaatca gagaaggaaa
     3361 tgtatttcaa agagctttag atgcaattct tgaaggtggt aaaatggggg tagatatggg
     3421 aatggctata atccctggag tcctagtagt atgtacattg gtgatgttat taacttttgg
     3481 accatctaca gaccctgtta caggtcaaga agtttataca ggagctgcat atgaaggaat
     3541 aaaattactt ccagttattg gagataaact tggatttata ttagaaccat tatttggatt
     3601 tacatcacca gaggcaatag cattccctat aacttcattg ggagctgttg gtgcagcaat
     3661 gtctctagtt ccagagttta taaagtcagg agctataact cctaatgata tagcagtatt
     3721 tacagctatg ggtatgtgtt ggagtggata tttaagtaca catattggta tgatggatgc
     3781 attaaatgct agacagttgg caggtaaagc tattctttct catacaatag ggggtttatg
     3841 tgcaggagca gctgctcact ttatatttac actcgtagga taaatgtaat cagctaagat
     3901 aaaaaataat atacaaaaaa atggttgttg atatgtaata agaatttaca tatcaacaac
     3961 catttttttc ttactaaaga taatttataa tacataaaca ttaagtggat attagagtta
     4021 agttatattt atgtaaaaag taacaaaaat ctgtagaaat ttatgacaaa atttagtaca
     4081 ctaactatat gttcttattt aaaatttaag gttagaatta ggaattacct agtagagtaa
     4141 gctttgatta ataaaagaat cataaaataa aaatattagt cataaagatt ataaataact
     4201 tacttaaaat aaagccatag aacttacaaa aattaaaaaa gcatataaat atggaggggt
     4261 acaatggatg aattatttca tataggaatt gatgtaggtt ctacaacggt gaaggttgtg
     4321 gtacttaata atagtaataa tatagttcat aaagaatata gaagacatta ttctgatgta
     4381 aaaaaatcag ttaaagaagt actaaatggt atatatgaaa aattgggaga tataaatact
     4441 accattataa taactggttc aggtggaatt ggaatatcaa aaaaattagg tgtaaaattt
     4501 gttcaggaag ttatatctag tacaaaatca atagagtatt ttcatcctga aactgatgtt
     4561 gtcatagaac ttgggggtga agatgcaaag ataacatatc taagtggagg aatagaccaa
     4621 agaatgaatg gaatatgtgc aggtggcact ggagcattca tagaccagat ggcatcactt
     4681 ttaaagacag atgccagtgg attaaatgaa cttgctaaag ggtataatgt tatatatccc
     4741 atagcttcaa gatgtggtgt ttttgctaaa acagatattc aacctcttat aaatgatgga
     4801 gccaatcaga ctgatatagc aatgagtatt ttcaatgcag ttgtcgtaca aactgtgagt
     4861 gtcctttctt gtggaagaaa aatagaagga aatgttgcgt ttttgggtgg tccattgtac
     4921 tttttatctg aacttagaga agcatttaaa agagtattgg atttaaaaga tgaaaacata
     4981 atcttcccag aaaatgcaca actttatatt gctattggcg caggattatt atcagtagat
     5041 gaagataata tacaactaaa atctctaata gaaaaattgg atagcataaa gaatattgaa
     5101 gatggagaag taaatttatt agaaccttta tttaaagata aaaatgagta tgaagaattt
     5161 tcaagaagac atgaaaaaga aaaaataaat tatgtggaca taaattcaat aaaaagtaat
     5221 tgttatttag gaattgatgc aggctcaact acaactaaag cagcacttat tgatgaggat
     5281 ggaagacttg tatattctta ttataatagc aatgaaggaa atccattaaa aacaaccatt
     5341 aaagttataa atgaagtata tgatatatta ccaaaaaata ttaaaatatt gagttcaact
     5401 gtaactggat atggagaagg tcttattaaa aaagctctta aaatcgataa cggagagata
     5461 gagactatag cacattacaa agctgctaaa tttttcaata aagatgtaga ttttatactt
     5521 gatattggtg gtcaagatat gaagtgccta aaaataaaag atggagtaat tgataatata
     5581 atattaaatg aggcttgttc atcaggttgt ggctcatttt tagagacatt tgcaagttct
     5641 ttatctatga caatagaaga atttgcacac gaaggtattt attctcaaaa tcctgtagat
     5701 ttaggttcaa gatgtactgt atttatgaat tctagagtaa aacaagctca aaaagaaggt
     5761 gcaagtgttg gagatatatc agcagggcta tcttattcag taataaaaaa tgctttattt
     5821 aaagtaataa agattagaga tttaaatgag attggtaata atattgttgt acaaggtgga
     5881 accttttata atgatttggt gcttagaagc tttgaaaaac taattggaaa aaatgtaatt
     5941 agacctaata tttcaggaat tatgggagct tttggttcag cacttatagc taaagaaaaa
     6001 tatgaaagtg gatacaaaac atccttgtta tctaaggaag agttgaataa tatcaagcta
     6061 gaagcaagtg taactagatg taaaggttgt tcaaatcatt gtcttcttac aataaataaa
     6121 ttttctgata atgaaatttt tatttcaggt aatagatgtg agaagggtga agctatttat
     6181 ggtgataaaa aaataggatt agaaaagaaa aaaccaataa atttatttaa gtacaaatac
     6241 aatagaattt ttagatataa accattggaa aaagaagaag caaaaaatgg ggaaataggg
     6301 ataccaagag tattaaatat gtatgaagat tatccatttt ggtttacttt tttcaatgct
     6361 atgggcttta gagttgtact ttcagatagg tcatcaaaac agttatatga aactggtata
     6421 actagtattg catctgaaac agtttgttat ccaggaaaat tagttcatgg acatatagaa
     6481 aatttaattg caaaaggtat aaaaaatata ttttatccaa gtgtaacaaa tgaaaataaa
     6541 gaggattcaa atgcagataa ttattataat tgtcctgttg taatatcata ttctgaagta
     6601 ataaaaaaca atgtagaaaa tataagaaat aagaacataa attatataaa tccatttata
     6661 agtctaaatg ataaggaaaa attgaagaaa agactatatg atgaattatc aagacatttt
     6721 tctgatataa aaataactaa agaagaagtt aatcatgcag ttgataaagc tactgatgaa
     6781 cagaatttat ttaaaatgga gatacaagca gcaggtgaaa gagcacttaa agaaataaaa
     6841 gaaagaaata tgaaggggat tgtgttatgt ggaagaccat atcatataga cccagaaata
     6901 aatcatggaa tgccagagct tataaattca ttagatatgg cagtgcttac tgaagactct
     6961 atatgtcatt tagccaatgt acaaagacca cttagagttg tagaccaatg ggtatatcat
     7021 tcaagattgt ataaagcagc tagttttgtg agagataaaa actatttgga acttgttcaa
     7081 cttaattcat ttggctgtgg attagatgca gtaactacag accaggttca agaaatactt
     7141 aatgaaaaat cgaagatata tactattata aagattgacg aaggcaacaa tttaggagca
     7201 gctaagataa ggatgaggtc cttaaaagca gctatgtttg agagagaaag caaaaatata
     7261 gatgtaaaaa atataaaggt aaacaggata cagtatgcta aaaataatca gataacgaac
     7321 aaacatacta tattagcacc acaaatgtct ccaatacatt tccaattcct tgaaaaagct
     7381 gttaacttaa gtggttataa tatagaaatt ctaaaagata ctgatagtag tgtaattgaa
     7441 gaagggttaa gatatgtaaa taatgatgca tgttatccag ctataatagt tgtaggtcag
     7501 ctaataacag cattaaaatc tggcaaatat gatttaaata acacttctgt tactataact
     7561 cagactggtg gaggttgtag agctacaaat tatattggat tcttaagaaa ggctatatat
     7621 gatgctggat ttaaagatgt tcctgtcatt gcacttagtg taaatggaat agaagatagt
     7681 ggtataatgg ataatatatc actaaaatta atcaatagac tctttatgtc tgttgtttat
     7741 ggtgatttgc tgatgaaagt actatataga gtaagaccat atgaaaaagt tactggaagt
     7801 gcaaatgctc tatatgaaaa atgggttgat atatgcaaat cttctcttgt taaagcaaaa
     7861 atttctgtat ttactagaaa tataaaagat atagtaaaag aatttgataa tttagagata
     7921 ctagatatca aaaaaccaaa agtaggtttg gttggagaaa tattggtgaa attccatcca
     7981 attgcaaata ataatttagt agatatatta gagcgagaag gtgctgaagc agtcgtgcca
     8041 gaccttacaa atttcttctt aagttgtgcc tttaatacta tatacaaaca cactcattta
     8101 gaaggaagta gaaagagtag aatgatagga gaggcattta tatacataac tggaatatat
     8161 caaagagtat ataaaaaagc acttgataag agtgagagat tttatgctcc agccaatata
     8221 aaaaatgttg ctaaaagtac agaaccagta gtgtcattag gtaatcaaac tggagaaggt
     8281 tggcttttaa caggtgaaat ggttgaatta ttaaatgaag gtgtagaaaa cattatttgt
     8341 atgcaacctt ttggatgttt acctaatcac ataattggta agggttctat aaaagaatta
     8401 aagaggttgt ataaaaatgc aaatataata cctattgatt atgacccttc tgcaagtgag
     8461 gtaaatcaac ttaacaggat aaaacttatg ctttctaagg cttttaaaaa tatataagat
     8521 ttatattttg atatgttaaa agttaatgaa tttaaaaata tactctatat taagtatcta
     8581 taaaaaagag ttatcctatt ttttagggta actctttatt agttaaaaaa ttatatgtat
     8641 actgatatta aaggggggaa gtaatatgag tttagtatat gatgtttata aatcaaaatt
     8701 aggaaatttg tacatattaa gtgaagatgg ttttatagtt agtatataca taggagatga
     8761 aaaatttaat aatttaaaaa atgaatgtat taaaaaagga attgatataa agagaagtaa
     8821 ggaagattta aaggaagcaa taagccagat agatgattac tttaatggaa aaagaaaagt
     8881 ctttaattta aatattcgta ttgaagggac tgaatttcaa aaatcagttt atagggaaat
     8941 gttaaaaata ccttatggag aaacattgtg ttattcagat atagcaagaa atataaacaa
     9001 tccaaaatca gtaagagcaa taggacaagc aagtaaaagt aataaaattc caataataat
     9061 accttgtcat agagtagttg gcaaaaaaga tattggtgga tatatgggga atcattctga
     9121 tttgaaagag atactattaa atttagaaag acagagttaa cttagaaaga aatgttttta
     9181 ttgttaatat ttgatataat aatagtaaaa agaagtattt tttacaaaaa caatgaagaa
     9241 tttattttaa tttacaaaaa tgagggggaa ttttaatgtg tgataaagtt atagcatcaa
     9301 aatatcatga gaagggattt aattgtgcag aatcagttat aaaagcatat aatgaggaat
     9361 ttaatacaga tataccagtt tgtttaggaa gtggactggg ttcagggtgt ggggttgcta
     9421 gtttatgtgg tgcagtaaat gcatcaaata taataattgg atatgtaaaa ggcagaaacc
     9481 acgaagatga aagtactaaa gctaaagtat atgctaaaga tttaactaca acagtaagaa
     9541 aagagtatgg ttcagagtta tgtatagacc taaaaaaaga tttagttgct tgtagagaaa
     9601 taatggattt cgcatatgat agtctaaagg aaactttaaa aaaagaatta taagaaaata
     9661 agctatcaag tataaattat ttgatagctt atttttatag tcgatactaa ataaatcaaa
     9721 tatattttag aattattatt tgtaaataaa cagcaaataa ttttaggatt tttatttaat
     9781 atagttt
//
LOCUS       DAEMIK010000152         9497 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEMIK010000152 DAEMIK010000000
VERSION     DAEMIK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.154"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            9..968
                     /locus_tag="KPK87_003194"
     CDS             9..968
                     /locus_tag="KPK87_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2539316.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKLLIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            1083..1868
                     /locus_tag="KPK87_003195"
     CDS             1083..1868
                     /locus_tag="KPK87_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539317.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            2160..3479
                     /locus_tag="KPK87_003196"
     CDS             2160..3479
                     /locus_tag="KPK87_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2539318.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3554..3982)
                     /locus_tag="KPK87_003197"
     CDS             complement(3554..3982)
                     /locus_tag="KPK87_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539319.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(4073..5449)
                     /gene="hydG"
                     /locus_tag="KPK87_003198"
     CDS             complement(4073..5449)
                     /gene="hydG"
                     /locus_tag="KPK87_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG2539320.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5450..6535)
                     /gene="hydE"
                     /locus_tag="KPK87_003199"
     CDS             complement(5450..6535)
                     /gene="hydE"
                     /locus_tag="KPK87_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG2539321.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6571..7779)
                     /gene="hydF"
                     /locus_tag="KPK87_003200"
     CDS             complement(6571..7779)
                     /gene="hydF"
                     /locus_tag="KPK87_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG2539322.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(7794..8045)
                     /locus_tag="KPK87_003201"
     CDS             complement(7794..8045)
                     /locus_tag="KPK87_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG2539323.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(8426..9466)
                     /locus_tag="KPK87_003202"
     CDS             complement(8426..9466)
                     /locus_tag="KPK87_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG2539324.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         3361 a         1326 c         1348 g         3462 t
ORIGIN      
        1 acttatcagt gatagaaagt aaaaagtcag aaaaattgtt actgtcaatt atatacataa
       61 tatttttggt atatatttta atgattataa gaataatttt atttagagat gtaccaattt
      121 acgctatttt caagggaacg tttaggaatg taaatttaat tccattttat acaatatatc
      181 aatttatagt agatagtaat cttgacttta tgaaagctac tgtaaatatt atagaaaata
      241 taggtatatt tattcctatg ggaatttttt tacctatagt atgtaaaaat ctaaataaaa
      301 aaactataat tatagtaatt atacttgtta gcctagcatt tgaattaaca caatatattt
      361 ttgctcttgg aagtagtgat atagatgatg ttattttaaa ttcattaggt ggcataattg
      421 gaattaatat ttacataaac atgaataaat tatttcccaa tgatattaac agatttaagg
      481 ctataatagg tactagttta gttttaggag ttataggatt aggagttatt tctaaaaatt
      541 atcataactt actaactttt aaatttagac ctgatagaaa aatatcaaaa ctattaattg
      601 aagaaaatag ggaaataatt aaagatataa ataaagatac agtagatata gttgggacat
      661 ttgagagctt taaaaaagga ataataacta ttaaagcagg ttctaataac aaagtaaaga
      721 gaccagagaa tttaattgat tctgatggaa atataagaat ttatttaaat gaaaatacaa
      781 aattagtatc ttatataatt accaaagaat caaaaatgga tatagttaaa tatgaggaat
      841 ttgatgtgaa gaatttagat ttattaagga gatatgatac tattaatgtt tggattgata
      901 aagaaaatca gccaaaggat aaacatggta ttatggctag taaattatta attggattgt
      961 atgaataaaa tattaaacaa gaataaaaga ataacaaata taaaaaacga aaaaactatt
     1021 tctataatta tttttacgtt gtgcataaaa taagaacatt agtgaaggag tagagataaa
     1081 ttatggagat aataaaatat atcttgtact tttttatata ttcattttta ggatggacag
     1141 tagaaagtat aggatgttct atagcaagca aaagaataat caacagaggt tttttaaatg
     1201 gtccaatatg ccctgtttat ggttttggag cggtaattgt gatatcacta ttgggaagat
     1261 ttaataatgt agtaatagta tttctattag gaatgatttt aactacgata ctagaatatt
     1321 ttacaggatt tattttagaa accttatttc atgctaaatg gtgggattat tctgacagaa
     1381 aattcaatat aaagggtaga gtatgtttaa aaaatgcaat atactttggt gtaatgtctg
     1441 ttttaataat aagatttata catccattta taaaatattt tgtaagtata attccatata
     1501 gaatattaat ttcaatggct ataattacaa cactttggac tatacttgat ttgattgtta
     1561 ctattataac acttaaaaaa ttagatataa agttaaactt attagacgat ataattacag
     1621 accttaatga tataaatgtg aaattggata aatttgatag aggagaaata caagctttat
     1681 ttaaaacaat aaataacgac gaattagaag ttagagaaaa aataaacaaa ataaatagta
     1741 agttggaccg tatagagtct aatattattt tacaaaaaag agttataaaa gcttttccac
     1801 atataaaaca taaaaaacaa caagagcaac tagaacattt taaaaaaatt attaatgaga
     1861 aaaggtaatt atacaattta atgaagacga atagaataat ggtatttata agatgatttt
     1921 cagaggactc tataaaaata gaatagagtc ctttattttt tgaaaaataa tgtttatttg
     1981 ataaaaataa aatatattgt ttctgaataa caaatatttt gaaaattata aaaacatata
     2041 ttgacaattt gtgtcaaaaa tactaacatt aagtatataa agaattttat atgagaatat
     2101 ctttataaat cgcaataatt ttaaaacata aatttattta aagaggggat gatttgccaa
     2161 tgagcgagat aactagtcaa atgataagta cagaagaaaa aaaatatgtt gctaaaactc
     2221 aaaagatacc ttattatcca gtagcattta aatcaggtga tggtgcaatg ctatatgatt
     2281 atgaaggtaa tgaatatgta gattttttag caagtgcagg ctctgcaaat gtaggtcatg
     2341 gtaataagga aatctcacaa gcagttaaag aacaaatgga tgatataaca caatatacac
     2401 ttgcatattt ccatagtgat cctcctgtaa aattagctga gaagcttgtg gaaatagccc
     2461 ctggagataa tgataaaaaa gtactataca gtgcaactgg ttctgcttgt atagatgctg
     2521 ctataaaatt agcaagaggg tatactggaa gaacaaagat aatatctatg tgtgaatctt
     2581 atcatggaag tacttatgga gctatatcta tatctgcttt aagtacaaat atgagaagaa
     2641 aaatgggtcc attgttacca gaagtttatc acttccatta tcctgataaa aatagaacag
     2701 ctaaagaatg tttagatgag atagaatatg cttttgcaca ctatctacca gcagaagagg
     2761 tagctgcaat atttatagag ccaattgcag gagatgcagg aataatagtg cctccagtag
     2821 agtgggtaca aggtttaagt aaaatatgta aagaaaatgg aatattactt gtaagtgatg
     2881 aaatacaaca aggtatggga agaacaggta agtggtttgg tatagaaaat tttggtgttg
     2941 aagcagacct tatagttctt ggaaaatctg ttggaggtgg attacctcta ggagcagttg
     3001 ttggaagaac agaaataatg caaagtcttg atgcaccagc acatttattt actttagctg
     3061 gaaacacaac agtatgtgtg gctgctttaa aatctataga gataattgaa aaagaaaact
     3121 tacttcaaaa gagtatagaa atgggagatt atattaaagc aggatttgaa aaactaaaag
     3181 aaaaatacga tataattgga gaaataagag gcataggatt atctatagga gttgatatag
     3241 ttaaaggaaa aggttctaat gaaaaacatc cagatgctac agctaagata tgttatagat
     3301 gtatacaaac tggattaata atgatattct taggacaatc tactttaaga gtgcaaccac
     3361 ctctagtaat aactaaggag caagtagata aagcaatgaa tatcatagat tctgcaatag
     3421 atgattattt aaatggtaga attggtgatg aagtatatga agtaactcaa ggttggtaaa
     3481 ataaatatta aatgaaaata ggagttatct caattttaga aaattatttc tgaaaagaga
     3541 taactctttt aagttaccca caaataagaa aattgtttga ttcattaaag actatatttc
     3601 ggatgtcgtt tttgtgagta tcagagacag tattacgaga ataattacct ttattcgtac
     3661 tatctgttaa tttagttata attaagatag ctaaaagaat aagaattact gtgtataaag
     3721 caataacata tggttttctt ttatatttgt atttgttatt gaaaaaagaa tggccataat
     3781 tatttttatt tactttagat gtatttgtat tctttttctt gttacctttc tttttatcat
     3841 ccttttttct atttttaggg taaggaatga ctttgtgtgt attatcaagt ttgatatttt
     3901 tatttttttg aatattgttt tctaggctag cctttttttc tccaaattga atagttttat
     3961 tggtattgtt gttatctttc aatgttaatc tccctccctt aaactttccc tttagataat
     4021 attactacta acctatggaa tatacaataa ataatatata agtaataaaa ttttataggt
     4081 ataaatctct ttctccacgc tctaaggcag tcatattgtt agatactatg tttcttatat
     4141 cttctctttc tatcttacct atttcttctg cgataagttt tttacccata attttaagtt
     4201 cttcatctcc ataatcgatt aaaaattcat ttaatgtagt aagagcattt ggtgtacata
     4261 cattatggat ttgtcgactt ttagctaagc tcatgaatct atcgccagtt ctacctttac
     4321 gataacaagc tgtacaataa ctaggtatat atccatcagt aatcagttct ttaagcacct
     4381 caacagggct tctatggtct ccaacttcga attgttctgt attgtcacca tcttcatatg
     4441 ctttatatcc tccaacacct gttagagaac ctgcactaat ttgtgataca ccatatttta
     4501 gtaactcatt tctcatttca gcagtttctc tagtggacat tatgattcca gtaaaaggaa
     4561 cagctaatct agttatagct actatttttt tgaacatatc gtcagaaact aagtgaggaa
     4621 aatcttctaa gctcatacct tcagcttttt tcaatctagg gaaagatata gtatggaaac
     4681 ctacaccaaa tttttcctct aagtgttcgt tgtgcatcat aagtcctaga acttcaaatt
     4741 tagggtcaga taaaccaaat aaaactcctg caccaacatc atcaacacca gcttccatag
     4801 ctctatcaaa agctgttaaa tggtaataat agtcattttt tatagattga ccatgcattt
     4861 ttataaatgt tggtttgtga taagtttctt ggaataatat ataagttcct attccttttt
     4921 cttttagtaa tttatactca tctacacttg tagctgctat gttaacgttt acccttctta
     4981 tatttccgtt ttcattatat gttgaataaa ttgcatctaa gcaatctaag atgtaattta
     5041 tatcacaatt tttagggtct tcaccagctt ctagagcaag tcttttatga cccatttttt
     5101 ctaatatttt aacctcttcc cttatctctt ccatggtaag tttttttctt ttaaatttgt
     5161 tgcatttact aaaaccgcaa taaacacatt catttacaca gtagtttgat acataaagag
     5221 gggcaaataa aaccactcta ttaccatata tttcattttt tatttgccca gctatactaa
     5281 ataatctatc tagttgtttt ttatcttcta cttcaagaag tgtagctata tctttataac
     5341 taagtttttc ccttctatca gccttatcaa gtaccttttc aatatcatca gaagttgaat
     5401 tttttgcatc ttctaataaa gaattaataa gttcatggtt tatgaacatt tacaaacgcc
     5461 tccattcaat attatcaccg cgagaatagt caacttcaag tcctaatgac ttgatgtttt
     5521 cttctaaact ttggtgatat tcacaagctt cttgaccagt aaaggctttg ttatcgtaaa
     5581 gagaatattg ttttctaaat tcttgaggag aaaggtttgg cataattaca ttgcaaccag
     5641 ctaaaagacc tgcatttcta ccactagggt ttatagatgc caaggctgtt gtagcgggta
     5701 ataaaacttt tggaagtaga agtcttgtaa ttgaaagcat aagtaatgtc ttctctaaat
     5761 ctccatgttt ataatcctta aaaattgtat catggtgtgg aatgaaaggt cctattccaa
     5821 ccatatgagg atttaactct tttaggtata ataaatcacg aacaaggtct tcgttgttct
     5881 gaaatggtga ttcaaccata aatccagcac ctacctgata accaatttct ttaaggtttt
     5941 ttaggcattc ttttctagtt ctaagctcaa tgtttggcgg atgtaatttt ttataatgac
     6001 tgtctgtagc agtttcatgt cttaaaagat atctatctgc accacataaa aagtattttt
     6061 tatacgaatc ataactcttt tctcctagtg atagagttat agcacaatcc tcatatttat
     6121 tttttatact tgaaacaatt tcacatattt tatcatcagt aaaatatgca tcttcaccac
     6181 cttgcaaaac gaatgttctg tatcctattt tataacctat atcacaacat tcaagtattt
     6241 catcaagtga aagcctgtat cttatgatat ttttattaga agattgtatt ccacaatagt
     6301 agcaattatt tttacaatag tttgtaagct ctataagtcc tctcaagtag actctatttc
     6361 cataatgttt atttctcatg tcgctagctt gtttataaag atagtttctt aaattttgtt
     6421 cgaagttata ttcctcattg tcgatatttt ctattaaata tagtagttca tcaaaactta
     6481 atgaattaat gctgtaaagt ttatctataa agtatttaat cttattttta gtcatattca
     6541 aatatgtaat tattaagtat agttaaattt ttaataattg aaaagttcaa gtgatctttc
     6601 caaaatacct tttacatatg ctattaagac accatagttt acaataggta cattgaaaga
     6661 ttttgccttt tctattctag ataacattcc agctctattc atcatacaag ctccacagtg
     6721 aactacaagg gcatatttat ttatatcttc agtgaaggat actccagaac taaattcaaa
     6781 agtaatgttt ttgccagttt tctttctaat catattagga atttttactg ttccaatatc
     6841 atcagtttgt ctatgatggg tacatccttc ggccatcaag attttatcac catcttttaa
     6901 attttctaaa gcataagctc cattaatcaa ttctttaagg tctccctttt gtctagcaaa
     6961 caatatagaa aaagatgtaa gaggaatatc ttttggtgta tctttatcta cttgtggaaa
     7021 tacttgagag tcagtaataa caagttttgg ttttttgcct aaattagata gagtttcttt
     7081 taaactatct tcttttgtaa ctatagaaat tgcaccatta tccaaaatat ctcttataac
     7141 ttgctgttga ggaagtataa gtctaccttt tggagctgct ttatctattg gaacaactaa
     7201 aacaactaaa tcatttggtt caattaaatc tgataccaat ttgaactcag tgctatcctt
     7261 aggcaaaact tttattattt cattttttaa attttcaatt ccaattttat ctgttgatga
     7321 gacagaaaca actggacatt taactttatt tttagttaag tttaaaattt ctgattgatt
     7381 cttaatagta tcaattttgt taagaattag aatatgaggg atattcttat cattaaattt
     7441 ttcaattagt gacaaatctt cttgtgatat accaatttgg caatccacta ctagtaaagc
     7501 tatatctgtc ttttctaaaa catctagtga tttacctatt ctaagttcac caagttcacc
     7561 aacatcatca agaccagcag tatctataag aacacaaggt cctataggaa ggatttccat
     7621 tggtctaaat actgggtctg tagtagttcc agcaatatct gagactattg cagcactttg
     7681 atttgttata gcatttatta tgctagattt acctgcattt cttttaccaa agagacctat
     7741 atgtactctt accgattgtg gagtagaatt tagactcatt tgtactcacc accttaaact
     7801 tctttttttg atatactagt ttttacagat acatgaggta tgttgcccaa tttaccagtc
     7861 aaactattaa tagtatccat ttcgccaaca actgctatac atacgattga gacaccttct
     7921 tcttcgcaag gaatgcccat tcttccttta ataatacctt taaaacctga tacaacctca
     7981 ttgaatttaa attgacattc ttttggctct tccaaaatag cacttataac tgcaaccttt
     8041 ttcataaata tcctcctaac tcagaaaata tgtcttaaac aaatccaaaa tcaaaaaact
     8101 tttctttgta aagaaaagtt tctacaaaag aaaagttatt aattacacaa gtataatgtg
     8161 ctatattaaa ctatttcctt acagattaga taactctttt ctggtagggt tagcttgtaa
     8221 aaatttaatt taacaacaag taactgaaga aaatttttat acaatttata taaattataa
     8281 taaaaattaa ctaaagaaaa ttgaagttac ttatataata gtagtaaatt gaaattaagt
     8341 ctatagaaaa accaattttt gtataaaaat aaaaattaaa gttaattatt tgttaagaat
     8401 tgactttggt aaaatatata attaattatt ctttaagcat ataatttata agtaatttta
     8461 gtgtattttc aatagcttct atatgtgttc tttcataatg gtgactattg tttgttccag
     8521 gaccaacaca tgctatttga acatcttctc cccaacgaat agcttctgaa gcatctgaac
     8581 catagaaagt aaatacatcg acagcgtagt ctaaattatt gtcttctgca acatcgacaa
     8641 gcttgtttct taaattaaaa tcatataatg ataaattatc ttttgcaact atagttacac
     8701 catgttcact agatgtttga cttggtccag taggtgctat atctattgct acaaattctt
     8761 ttgttttttc aggtaaacat ttagaaacac catgtccaat ttcttcataa ttacttatat
     8821 aaaagttagt agtatgctta ggtgttaaat tgttttcttt aaaatatcta catatttcaa
     8881 gaaccattgc tacagctgat ttattatcta tatatctaga ttttataaaa ccagattctg
     8941 tagcctcaaa tctatggtct aggcatacaa aatccccaac acttattcct aaatctaata
     9001 catcttgctt tgtaaatact ttttcatcaa gtctaataat cattgtttct tcatttcttt
     9061 cagtggcagc ttttgtttga ccatatatat gagtagaagc atacttaaat acaacacttc
     9121 ctctgatttt tttacctttt ctagttataa cagtacagtt ttctccctct atagctgccc
     9181 aacatccacc accaacttta tggtatttaa gagtaccatc agatgatatt tcttttacta
     9241 tagctccaag tgtatccata tgtgcagata ttgttatttg atttgaatca tcttcaccag
     9301 gaagagtagc tataagtgca cctttttttg taagaatagt gtcaagacct aaattttcaa
     9361 aatctttttt gatttctaaa actgcatttt cagtatatcc agctgggctt ggaatgtcca
     9421 aatatttttt cattaaattt atagtattag ttaaatttaa attcatattg tttcctccta
     9481 aataaatata tttatac
//
LOCUS       DAEMIK010000153         9403 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEMIK010000153 DAEMIK010000000
VERSION     DAEMIK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.155"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            391..1596
                     /locus_tag="KPK87_003203"
     CDS             391..1596
                     /locus_tag="KPK87_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2539325.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1667..1888)
                     /locus_tag="KPK87_003204"
     CDS             complement(1667..1888)
                     /locus_tag="KPK87_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539326.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2029..2628)
                     /gene="recR"
                     /locus_tag="KPK87_003205"
     CDS             complement(2029..2628)
                     /gene="recR"
                     /locus_tag="KPK87_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG2539327.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKKEIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2641..2991)
                     /locus_tag="KPK87_003206"
     CDS             complement(2641..2991)
                     /locus_tag="KPK87_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG2539328.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3050..4687)
                     /gene="dnaX"
                     /locus_tag="KPK87_003207"
     CDS             complement(3050..4687)
                     /gene="dnaX"
                     /locus_tag="KPK87_003207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG2539329.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4764..5028)
                     /gene="ffs"
                     /locus_tag="KPK87_003208"
     ncRNA           complement(4764..5028)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPK87_003208"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5066..5157)
                     /locus_tag="KPK87_003209"
     tRNA            complement(5066..5157)
                     /locus_tag="KPK87_003209"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5121..5123),aa:Ser,seq:gga)
     gene            complement(5158..5613)
                     /locus_tag="KPK87_003210"
     CDS             complement(5158..5613)
                     /locus_tag="KPK87_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG2539330.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5934..7205)
                     /gene="serS"
                     /locus_tag="KPK87_003211"
     CDS             complement(5934..7205)
                     /gene="serS"
                     /locus_tag="KPK87_003211"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG2539331.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7331..7536)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7664..8545)
                     /locus_tag="KPK87_003212"
     CDS             complement(7664..8545)
                     /locus_tag="KPK87_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG2539332.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8557..9066)
                     /locus_tag="KPK87_003213"
     CDS             complement(8557..9066)
                     /locus_tag="KPK87_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG2539333.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIKAIVK
                     ELKDNGILS"
     gene            complement(9193..9309)
                     /gene="rrf"
                     /locus_tag="KPK87_003214"
     rRNA            complement(9193..9309)
                     /gene="rrf"
                     /locus_tag="KPK87_003214"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3003 a         1647 c         1007 g         3746 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttaattttt ttatttatca tgtattcttt atctttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatcaaaat ataatataaa
      361 tactatgcta cattttagga ggattttatt atgagcaaaa aagtattaat aatgtctgct
      421 tccactggtg gtgggcataa tagggctgct ctagctatta aagaagaact tacttccaaa
      481 acacttgatg gagagccaat tgaatgtgaa ataatagata gtttgaaatt agttaataat
      541 acaatggata aaataatttc aagaggatac gaaaaatctg ctatatatac tcccaaagcc
      601 tatggaagtg tatatagatt atctgaaaca aatcttttgt caaaaaatga atttaaagat
      661 aatctactaa taacattcat ggctaaaaaa tttaaaaagc ttattagaag cgaaaaacca
      721 gacttgatta taggaacgca tccttttcca atgattgctc taagtacatt gaaaaaaaac
      781 tttaatttac ataataatga atcaaatgct tacacagagc atttttataa acattataca
      841 aataccatta atgttcctcc gcttatttct gtattaaccg attatactac tcattctaca
      901 tggatacaaa atgaaattga ttactatatt gttggtcatg aatatgtaaa agaattattg
      961 gtgtttgatg gagttgaacc gagtaaaatt agaacatttg gaatccctgt tgaaaaatct
     1021 tttctctctc acagagataa agatattgtt ctttctgagt tgaatttatc tcctgataaa
     1081 ttaactgttt tacttatggg gggtagcttt ggggctggaa atataaaaga gacacttgat
     1141 gaattactgg atactgatag agactttcaa atacttgtaa ttactggtaa gaatgaaagc
     1201 ctaaaagaaa aaatcgaaaa aaaacttatg tctcgttatc atgataaaaa tgtatgtgtc
     1261 cttggataca ctgataagat gaatgatatt ttagcttcta tagatgtatt agtaagcaaa
     1321 cctggtggac ttactacaac tgaggctctt ttaaaagatg tacctatgat agttccttat
     1381 tatataccag gtcaggaaga agagaatctt gatttcttat ctaattgtgg tgcagcactt
     1441 agaactacaa aaaaatataa cttgtcaata ttactaaaag ttttaataga tgaccctagc
     1501 agattagaaa tgctaaaaag aaatattaaa tcaataagaa agtcaaattc agcccaaaat
     1561 atagctaatt taattttaaa tataatttct ggttgataaa ataaataaaa tccctatagt
     1621 atatatatta tctattaggg attttttatt ttctaattat ttaatattaa attggttcat
     1681 aaaagtcttc ttcataatca tactgataat ttttataatt atttctaaat tcagcatttt
     1741 tatttcgtac atttttttta gtttttttct tattagaaaa acttatatcc tcaaaatcat
     1801 catatttagg tttatctttt gatttcattt ttttaactct tttttctttc attgcattat
     1861 gttcttctaa gttcataatt ggtttcatgt tgcaccccta tcaaaatttt taaatgattt
     1921 atacaacaaa ttatacaata cagaagaaat ttattcaata aatctaaatc aaaattaatt
     1981 tgaatttaac tcaaaaaact acaaaaatcg aggtattaac ctcgattttt atatttctct
     2041 tctgccttct agtgcttttg atattgtgac ctcatctgca tactctaaat cgcctccaac
     2101 aggaagacca tgagctatcc gagtgacttt gattcccatt ggttttacta acctagcaat
     2161 atacatggct gtagcttcac cttcaatagt aggatttgtt gccattatta tttctttgac
     2221 ttcctgattg ccaagtcttt ggataagttc ctttattttt aacatatctg gtccaattcc
     2281 atccattgga gaaattactc catttaatac atgatactgt cctttaaact ctctcgttct
     2341 ctccatagct gctacatctc ttggatcttc cacaacacat ataacacttg aatctctact
     2401 tttatttgaa cacatgctac atgggtctgt atctgttata ttacaacaaa ttgagcaata
     2461 tcttatttct ttttttgcat ctataatcgc tttagataaa gcttccacat cattagtatt
     2521 catattaata atatgaaaag ccaatctttg agcagtcttt cttcctactc caggaagctt
     2581 tgaaaattcc tctataagtc tcgttatggg acctgtataa acttgcataa ataccaccta
     2641 ctagaataac ccaggtatat tcatacctcc agttacctta ctcatttgac ttgcttgtat
     2701 atcatcaata tttctcaatg cttgatttac tgcacttaaa actaaatctt gtagcatttc
     2761 tatatcatct ggatcaacta cttctggctt gatagttata tctataactt cttttttacc
     2821 attaactttt acagtaactg ctcctccacc aactgaagct tctacttctt tactttctaa
     2881 ctcttgttga gctttttgca tattttcttg cattttttga gcctgcttta acaagttgtt
     2941 catatttcca ccaccaggca tcattccgcc tccaaaacct tttttagcca taaacactat
     3001 tcctcctaaa cttattttca ttttattatt atatcataat agtattttat tatttactct
     3061 cattttcatc aatactatct tttatttcta atatctcttc actcactatg ttctttaata
     3121 tttcctcacc aatgtccttt ttctctttaa cttcaagatt tatatcttta acttctgatg
     3181 tcagtactat ttttacatta aaacttctat ttaaaacatc tcttattgtt ttttctaaat
     3241 aatttattgt atcttgagag ctcagtctag tttttgcaaa atcaaattta tcatcaaata
     3301 taacatacaa catattagaa tatacattaa aactttttac ttcacttaag agtgcgtata
     3361 ttggcatttt tttatctttt ttaatttgtt taagtatatt cttccaagat gactctataa
     3421 gtataacgtc ctcatttttt acatcttcat aaactacatt ttcttgtttt acatctattt
     3481 ctctatcgac ctcatactct ttttctttat tattcccaat tttttcagac ttaaagttcc
     3541 ccagttcaat cattttttct aagttctcaa ctctttttat aagagcttct ttactttcat
     3601 caaacattgg ttgagcaatt ttcattatgg ttatttccac taaaaccctt ggatttgaag
     3661 atgactttat atcatcctga gttattgata atatatttaa aatccttatt aaatcattta
     3721 tatctatatt tttagactga attttaagtt gttctatagt ttcttctggt aatgaaatta
     3781 tttcatccaa ttctgatgaa accttacata ccattaaatt tctaaaatga tcaattaagt
     3841 catttataag atttcttata tctttgcccc ataagacaaa ttcatttaat atctcaagag
     3901 actttttagt gtcctgctct acaatacatt gagacaattc aaataattgt tctatattta
     3961 cgcttcccat taattcaaca acatctttat aatctatctt actttctcca aaagacatac
     4021 attggtctaa tatactaagt gcatctctaa gagctccttg agaatttcta gctattaaat
     4081 taagagcttt atcatctata tcaattcctt catcttcaca aatctttttc attctaaggg
     4141 tcatatcttt aactgtaact cttttaaaat cgtatctttg gcatcttgat aaaatggttg
     4201 caggtatttt gtgaggctct gtagttgcta atataaaaat tacatatgat ggaggttcct
     4261 ctaaggtctt taataatgca ttaaaagctc cttgagacaa catatgaact tcatctatta
     4321 tatatacttt atatttagca ttggctggag aatatttcac actttctcta agctctctta
     4381 tatcatctac actattattt gaagctgcat ctatttctac aacatccatt gtattatctt
     4441 ttaatacaga ttcgcatatc tcacactcat tacacggttc ttcatttatt ttattcaaac
     4501 agtttactgc tcttgaaaaa atctttgcag tagatgtttt accagtacct cttgttccac
     4561 aaaacaaata agcatgtcct atattatcac tatatatttg attctttaaa gtcttaataa
     4621 tatgatcttg accaataaca tcttcaaatt tttgtggtct atatgctcta tataatgctt
     4681 tatgcatctt tttacctctc tttagcaaaa ttaataaaaa aatccctcat tatctaaggg
     4741 attagtcttt tatatattaa atgctgtaca ccatctatcg acttaattcc ataggcgtta
     4801 ccaaaacagt tagctcaact taggcacccc cacggcacac aaaagtgatc acttaccgct
     4861 gcttccttcc ggatctgacg gggttcatga gctttcattg cgcaggacct aagcatcaac
     4921 accacttatt tagggcggcc ctacagaaaa taagccttta gatggaattc aatcctgcta
     4981 tagcggattg caggttacag ggcaccgcta cctccccatc tagtacagca aaattacaac
     5041 catatttaat tacttttatt gtttctggcg gagagagtgg gatttgaacc cacgatacag
     5101 ttttacccat atactcgctt tccaggcgag cgccttcgac cactcgacca tctctcctta
     5161 atctcttttt ctcaatgcct taaaaaattc ttgcaatatg atcgagcatt cttcttgcaa
     5221 aacatcaaaa gctactttta cattattatt attaaaataa tctaatttaa actcatgctg
     5281 tttttctata tatgaatttt ttacatgtct tgtccctatt ataagctttt ttattctaga
     5341 attaactatt gcaccactac acattatgca aggttccatt gtaacatata aatcacaatc
     5401 agtcaacctc caaccaccta atttttctga tgcttgtttt atcgctaaaa tttctgcatg
     5461 tgccgtagag tcttttaacg tttctgtaag gttatgagct ctagctatta tttgattatc
     5521 tttaactata atagcaccta ttggtgtttc ttttttatta tatgctttat atgcttcttt
     5581 taatgcttct ttcatataaa atgaactttc cataattcct cccaaaactt ataacaaact
     5641 tatcttatca taattcattt tataattcaa cttaatttta ttttgtttat ttaattatta
     5701 ctatatatag ctatattttt tttaaattag ttctaaaata atttttagat attcatacaa
     5761 gagtgtttta taaaaatatt tagtgtttta tgatgaaaat tctgctgtgt aaattaacat
     5821 aaaaaaacta tgaaattaat atttataata atttcatagt ttttttattt attgtttact
     5881 cataatatat tctcttcatc aaagcatact ctacatatac aattaatata aatttatttt
     5941 ataacatcaa cacccatata aggtcttaat tcttctggaa ctactacaga accatctgcc
     6001 tgctgataat tttctaatat agcagccaaa catcttccta tcgctaatcc agaaccattt
     6061 aatgtatgaa catattctgc ttttgatttt ttatcccttt taaatcttat tcctgctctt
     6121 cttgcttgga aatcttcaaa atttgaacaa gaagatattt ctacatatct attgtagctt
     6181 ggcatccaaa cttctaaatc atatttaaat gcagctgtaa atcccaaatc tcctgtacat
     6241 atttttacaa ctctatatgg aagacctaac atttgtaaca tagtttcagc atcatgagtt
     6301 aatgattcta attcattata agaatcttct ggtttaacaa atttaactaa ttcaacttta
     6361 ttaaattgat gttgtctaac taaacctctt gtatctcttc ctgctgaacc tgcttcagat
     6421 ctaaaacaag gagtatatgc acagtattta attggaagct ctgcaacatc taatatttca
     6481 ttagcatgta tattagtaac aggaacttca gctgttggta ttaagaaata ctctaatcct
     6541 tctattttaa acatatcttc ttcaaattta ggaagttgac cagtccctat aaaactattt
     6601 ctattggcca taaatggagg taagacttcc gtatagccat gcttagctgt atgagtattt
     6661 aaaaagaaat tcattaaaga tctttctaat ctagctccta atcccctata taaagtaaac
     6721 cttgacccag ttattttccc tgctctttca aaatctaaaa tacctagacc tgtacctata
     6781 tcccaatgcg ctttatgctc aaaatcaaat ttagtaggtt ctccccaagt tcttatttga
     6841 acattgtctt catctgtttc accttgtggt acctctggat gaggaacatt tggtattctc
     6901 attaatgtat attccatttt tgcttctact tcttttactt tctcatcaat accttttatc
     6961 ttatctgata actctttcaa tcttgccttt tcttctgtta catcttttcc ttcttttatt
     7021 aattgaggta tttttttaga atttacattt aactcatttt tcataacttc aacttcttga
     7081 agtatttctc ttcttttatt atctaattcc accacagcat caaggtcaaa ttctctttca
     7141 cctcttcttt ccatagcttt tttgatatca tctagatttt ctcttattct tttaatatct
     7201 aacatattaa ttcctcctaa taattttgat aatataagtt ataattccaa cttaatttac
     7261 aaacattata cttgtaaact cttagtttaa acaaatttta aaaataaaaa aagacctccg
     7321 tttaccctat aaaagggacg aaaatctttt atccgcgttg ccaccctaat tgactatact
     7381 aaaatgtata atcctctcag acctataacg taggtaaacg tctagcttta actagaagct
     7441 ctagagtaga ttcaaaaacc attgtaatga gttttcacca tccactcatt ctctgaatac
     7501 aatctgtttt ttactatttt ccttcacagc tttttaacta attgttttat ttgataatca
     7561 ttatattata tatattaaca tctttcaata ctattattca aattttttta gaattttctt
     7621 taatttttta tattaaattc attttttcta tttaattaag accttatctt ttatccattg
     7681 tatttagatt acctgtatta ttaaataata atgaagaagg aacaaatgga acttttatat
     7741 cattctcatc aaatttattt tttacctttt ccattacatc aaaatataca tcccagtaat
     7801 catcagtttt tacccaaggt ctagcaacta tttgcatggt gttatttcct atagagttta
     7861 aacctattat aggctttgga tcatttaata ttcttttttc gtttgtaaaa atttcattta
     7921 atatagactt aactacatct atatcactat catatgaaat attatatgaa aaatctattc
     7981 ttcttttgtt ttgatgtgtg taattaatta tattattact tgtcagttga aaattaggta
     8041 taattatagt tttattatct ggtgtcttaa gaacagtgct aaatatttgt atgtcagaaa
     8101 ctgtaccttc tacacctgaa ccttgaatat aatcacctat attaaatggc ttaaaaaata
     8161 aaataatcat accagagcct aaatttgata atatttcttt aaaagctaaa cctatactaa
     8221 atccagcagc tcctaacaca gctacaactg atgtagtttg tatccctata atacctaagc
     8281 ttatcaatga tataatagat aacatagata tatatattcc atatacaata aaattattta
     8341 cacctttact cattccatat ttgattagaa actttgaaac catccttctt actattttag
     8401 atatataaat acctatcaca aatacaatta ttgcatatat aatatttggt atattatcac
     8461 taaaattatc gagcatattt tctaaagttc tcttagttat attgctattt gttgaggcag
     8521 tcttagccaa tgataaaaaa ttcattcaac tcctccttat gataatattc cgttgtcctt
     8581 taactctttt actatagctt ttatagtttc ttcttttatt gcctcaattg tatgaatatg
     8641 gctacattct gtcagaaccg ataattgttt aaattcattt gtctcaactt tcttcataaa
     8701 gttgtctata tccattttag aataaatatt aagttctgca gttatctgac catatgttgg
     8761 atgatttacg atgacatctt ttataatacc acctaaatcc acaattatct ttaattcatc
     8821 ataaatttct ttagaatcct tatgattttt acactttatt tgttttgtaa aatacggttg
     8881 attatatatt atatatcctt gaggtgtagc tattatttca aatcctctag ctcttataag
     8941 ggctatatct tgaactatta cttgcctact tacatttaat agttcagcta gtgttccacc
     9001 ttttacagga tgcttagatt cttttaaaat atctattaat ttacttcttc tttcattaga
     9061 attcatatta caccttcttt ccaaataaaa actttaccta tactatataa ttttatattt
     9121 tatcttatta taaatcaata ttgactttat attctattta atcttaactt ttataataaa
     9181 taaaaaaaga ctacgtggct acgtcctact ctcccaggca gtttcccacc aagtaccatc
     9241 agcgctagag agcttaactt ctgtgttcag aatgggaaca ggtgtatcct ctctgctatt
     9301 gtaaccacat aatctttatg cttaatattt ctattaagtt tttaataagt ttatagaatc
     9361 tagtaatact agtttcttta gagttagcac tctaaaaact aca
//
LOCUS       DAEMIK010000154         9179 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEMIK010000154 DAEMIK010000000
VERSION     DAEMIK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.156"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(104..838)
                     /locus_tag="KPK87_003215"
     CDS             complement(104..838)
                     /locus_tag="KPK87_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG2539334.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIATLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            963..1679
                     /locus_tag="KPK87_003216"
     CDS             963..1679
                     /locus_tag="KPK87_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2539335.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1813..2505
                     /locus_tag="KPK87_003217"
     CDS             1813..2505
                     /locus_tag="KPK87_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539336.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2495..3676
                     /locus_tag="KPK87_003218"
     CDS             2495..3676
                     /locus_tag="KPK87_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2539337.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLVTNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3958..4413
                     /locus_tag="KPK87_003219"
     CDS             3958..4413
                     /locus_tag="KPK87_003219"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG2539338.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4403..5128
                     /locus_tag="KPK87_003220"
     CDS             4403..5128
                     /locus_tag="KPK87_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG2539339.1"
                     /translation="MGNNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5100..7481
                     /locus_tag="KPK87_003221"
     CDS             5100..7481
                     /locus_tag="KPK87_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG2539340.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPIVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7686..8915)
                     /locus_tag="KPK87_003222"
     CDS             complement(7686..8915)
                     /locus_tag="KPK87_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2539341.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         3603 a         1055 c         1551 g         2970 t
ORIGIN      
        1 aagataataa catataataa ataaaaaagt aagattgtgt agattatata aagattattt
       61 acaattaata caatcttgct tttttattta aaaattttaa ttattattta aaatttttta
      121 tcttctcttt atcccatata aacaatttct cagaattatt taaagcacca aatacatttc
      181 ttttaaaatc aggcatctct ttatttaaag tagcaataag attttttatg tactctctcc
      241 tagtatgacc atttgcaggg cagggatttg tgataatagg atagttagaa tcttttgaaa
      301 ttttcttagt catatactct tctatgtaaa ccataggtct tataactgtt aaatcctgtc
      361 tgtctaggta tgtctttggg gaaaaacaat ttactctacc ctcataaaac atagacatta
      421 aaaatgtctc aattgcatca tctttatgat gaccaagagc tactttatta catccaagtt
      481 ttttagcatt atcgttcaaa gcacctcttc ttaaattagc acacaatgca caaggattct
      541 tttcttgtct tatatcaaat acaatttctt ttatgtcagt ttgaaattca taaaaaggaa
      601 catttatttc tttacacaat ttatctagtg gagaattatc aacacctcca gtatttaatg
      661 ttatggctat caattcaaag ttttgtggag aaaatctttg ataactttta agtatatgaa
      721 gtagagttaa actatctttt ccaccagaaa gtccaaccgc aattttatca ttttcttgta
      781 tcatatcaaa atcttgtata gccttacgag ctttacttag aagtttttgt atagacattt
      841 tatcaccatc ctttaaaaat attaaattta agatttattt aagatttact tataaaatac
      901 ttaagtctta tactctaaaa ttaacattat agcaaacata acaaaaagga gaagatttaa
      961 ttatgaaaaa atttaaatta ataggagcca ttattttagt agtatttata agtgtattta
     1021 tatctaaaga tagattactt aagaaagata aagatccaat agtagctgct caacatgatt
     1081 cagaacaaga tgttgaggca aattctcaaa ataaaaatca ctcaaatagt atatcaaata
     1141 tattattggt aaacaagaca aatggaatta gcaaaaacta tactcctgaa aatattacta
     1201 aggtaaacat accttttgtt gaagaagcaa cagaggaaga aaaacaaatg gcaggagaac
     1261 ctgcaaaggc agttgaagat ttagttaagc aagctaattc agaaggaata cagtttttag
     1321 ggtcttcagc atacagatct tatgatactc aactggatac ttatacaaga agggtaaaat
     1381 ctcaggggcg agaaaaggca gatgcatatg tagcaaagcc tggttacagt gagcaccaaa
     1441 ctggactttg tattgattta acaaatccag aaagatggtt tgttggaagt acaaaagaag
     1501 ctaaatggtt ggcagaaaat gctcacaagt ttggatttat aataagatat cctgaaggta
     1561 aagaagatat aacaggtaca gcatatgaac catggcatat tagatatgtt ggtaaggatg
     1621 ctgctgagga aatttattca aaaggattaa ctttagaaga atatttacaa aataaataat
     1681 ataaatgtgt aaataatttg tattatattg gaaatttaga aattaataaa tttatagatt
     1741 acactaacta aaatatagta taattttata tgaataaagc tatatacaaa gattaagtta
     1801 ggatgtgtaa aaatgaataa tacagttctt gttgtggatg atgaaaagga aataagagac
     1861 cttatagaga tatatctttt aaatagtgga tataatgtaa ttaaagctga agatggaaaa
     1921 gaggctctag atattttaaa aaaggaaaat attcatttaa tgatattgga tataatgatg
     1981 cctaaaatgg atgggataga agtttgtaga aaagttagag aaagtttgaa tataccaata
     2041 cttatgttaa gtgcaaaatc agaagacatg gataaaatac aagggataat gactggtgca
     2101 gatgactatt tgacaaaacc atttaaccca cttgaactta ctgtgagagt gaagtcgctg
     2161 attagaagag catattattt tagtggtgct aataatgaag ataaagatgt cataaaggtt
     2221 ggagtagtaa ttatagataa ggaaaaacat agtgtaacag tggctggaaa tgaaataatt
     2281 ttgacatcaa gagaatttga tatattatat ttactagcaa ataataaagg cagagtatat
     2341 agtacagaag aaatatttga aaaggtatgg aaagagaaat attaccaatc taataatact
     2401 gtaatggttc atatgagcag aataagggat aaaattgaaa aatatacaga tggagaaaaa
     2461 ataattcata ctgtatgggg agtaggatat aaaattgaaa aataaaagtt tattaaatca
     2521 tatgtttcga cccatattag atcttataat aagctgtgct ttaacttcaa tgcttatttt
     2581 aacaagtatg tatataataa aatcattata tgtcaatagt gatatgtctg tagttaatca
     2641 acttgtatat caagcaagag atttcagaag tcttataggg tatgatttat tttatccatt
     2701 tttatttaca atcacatcat tagcaattta ttccattata tcgtacaaga gaaataagca
     2761 aattgtagat tttataagaa agactaatag aaaaaatcaa aaattagaaa tcattaaaag
     2821 tgctgtaaat ttaatggatg aaggtaactt agaaaaaagt atagatattg agtatgattt
     2881 agatatatta aaggataaag aaaaggatga tattgatgaa ctagcacata aaatcaataa
     2941 tatagtccat caacttagaa atataactat tgaagagaga caagctcagc agacaaaaac
     3001 agatttagta acaaatgtgt cacatgactt aagaacacct ttgacatcca taatgggtta
     3061 tttaggagtg atagaagaag gtaagtataa agatgaagtt cagatgatgt attatgttga
     3121 tattgcatat gaaaaatcga aaaatttaaa tgttcttata aatgatttgt ttgaattgac
     3181 taaaatgcaa aataatacaa taaaattaaa caaaatagaa ataaatttag tagagttatt
     3241 aagtcaagta gtatctcaat ttgaaattta ttttaaacaa gaatttatgg ttagtagaat
     3301 taattttaat gaagaaaaat taattgtaaa agctgatcca aataaattgg taagagcatt
     3361 tgaaaatctt ataactaatg ctgtgaaata tggaaaagat gggcattatg tagatatagt
     3421 cacagagaaa aaggaagata tggcggttgt aaaagtgata aattatgggg aaccaattcc
     3481 agtgttagat ttaccaaata tatttgatag attttataga gttgaaaaat cgagaaatag
     3541 aaatgttgga ggttctggat tgggtcttgc gattacaaaa aatattgtaa atttacatga
     3601 tggagaaatt actgtttcaa gcgataaata tcaaactgtg tttgaagtac aactgccaat
     3661 aatacaatca gcttagttat aaaataaaag tacgattatg tgatataaat attataacaa
     3721 taaagtgttt acaaaaataa aaaagtaata tataatttaa gtcagtttaa taagatactt
     3781 atcaagagtt aggggagaga gttagctcag tgatcctata ccaacctttt atcaggtggt
     3841 cctgctaaca acgatgagat gacttgaaat aatatactct tcttatcaat aatagatagg
     3901 aagagttttt tttagaatta atgtaacaaa ttttaaagta taatggagga ttaaataatg
     3961 gaaaaagtag aaagttttaa gctagaccat acaaaagtaa aagctccttt tgtaagaaag
     4021 tgtagtgtac ttgatggagt aaagggagat aaagtaacta aatttgactt gagattttta
     4081 cagccaaatg tagaaagttt tggaactgct gctatgcacg gtttagagca tcttttagct
     4141 acttatttaa gagatacatt agatggtgta atagacttat ctccaatggg atgtagaaca
     4201 ggattttatt taatactatg gggagatgta gatgccaaaa cagttaagat tggtttagaa
     4261 gaagcattaa aaaaagttct tgaatcagat aaaatgccag cagctacagc tatagaatgt
     4321 ggaaactaca gagacttatc tttatttggg gcaaaggaat atgcaaaaga tgttttagat
     4381 aaaggatttt ctttaaatat atatggggaa taatttaatt ggaagagaag atataagcat
     4441 aaatcaaagt gaagttttaa gatatttaca atacagaggt caagagatag ataaagacct
     4501 taaatatact attgatgaat gtgtagctat aactaaaaat aaaataaatc ctagatattt
     4561 atctaggatt tatcctatta aaataaataa agataatgtt gtagaattag agggaacaaa
     4621 tttaacatta gagagtaaag atgtatatga acttttaaaa gaatgtaatg agtgtgtaat
     4681 tatggcagct actataggga tagatataga aaaagaaata agacgacact cttattcaaa
     4741 tttaacaaaa ggactaataa ttgattcatg tggaacaaca gcaatagaag aagtgtgtga
     4801 cattgttcaa aataatttag aaaaggtttt tttaaagcaa gataaattta taactatgcg
     4861 atatagccca ggatatggag atttaccaat agaaaaaaat atagatatat taaacgtttt
     4921 gaatgctcaa aaacaaatag gtctaacaat tacttctaat ggaattatga taccgagaaa
     4981 gtcagtagtg gctattatag gtatttctaa tacaagaata aataaatcta agaaagaaaa
     5041 gtcgtgtgaa aattgtaaaa actatagaaa ctgtacatat agaaaaggag cgaactcttg
     5101 tggaaataag acaatatctg aaaaataata tattaatatt tgatggtgca atgggaacaa
     5161 tgcttcaaca aaaagggtta aagttaggtg aaaatccaga agtatttggt cttcaaaatc
     5221 cagataaact tatagaaata catacagcat acttagaggc aggttcgaat gtaattctta
     5281 caaatacttt tggatgtaat gaactaaaat tagatagtaa gtatacggta gaagaagtga
     5341 ttgacaatgc tgttttggtg gctagaaaag ctatagaaaa tgtagataat actaaaccaa
     5401 gatatgtagc tttagatatt ggtcctatag gagaaatgct agagcctatg ggtactttaa
     5461 gttttgataa agcatatgaa atatttaaaa gacaagtact acaaggcgtt aaatctggag
     5521 ttgatgtaat agtaatagag actatgatgg atttatatga agctaaagta gctgtattag
     5581 cagcaaaaga aaactctgat ttacctattt tctgtactat gacatttgat gaaggtggaa
     5641 gaagttttac tggatgtatg ccagaatgta tggtggctac tatagaaggt ctaggtgtag
     5701 atgcaatagg agttaattgt tcattaggac ctaaacaact tttaccaata gttgaaaaaa
     5761 tagcatcaag agctacagtc cctgttatgg ttcaagcaaa tgcaggtctt ccaaatatag
     5821 ttgatggaga ggctatatat gatgttgatg caaaagaatt ttttgaaggg gttaaaaagt
     5881 tcgttgaagt tggtgctact ataataggtg gatgttgtgg tacgaatcca agttttataa
     5941 aagaaatatc tgagaatata aattctgtta caaagggatg tatcgagaaa attgataaat
     6001 gtgtagtctg ttcaccatca aaatttgtgg aagtacaatc gcctatagtt gttggagaaa
     6061 gattgaatcc cacaggtaga aaatctctac aagaagcatt aaaaaatgaa aatgttgatt
     6121 atgctataaa tttaggttta gaacaagtaa atgctggagc acaaatactt ggagtaaatg
     6181 ttggtcttcc agagattgat gaaaaaaaac taatgccaaa attaataaga gagattcaag
     6241 cagtagtaga tacaccttta caagttgatt cttccaatgt agaggcctta gagcaaggac
     6301 ttagatatta taatggtaga acaatagtaa actctgtaaa tggcaaagag gagtctttag
     6361 aaagtatatt accaatagtt aaaaagtatg gttcatgtgt ggttggactt acacttgatg
     6421 aaaaggggat acctaaaaag gctgaagaaa gatttgaaat agcaaaaagg atagttaata
     6481 gagcagtttc ttatgggata aaaccaaaag atatatttat agactgttta tcattaactg
     6541 tttcagcaca acaagaagaa gcaatagaaa ctataaaagc tataacaatg gtaaagacac
     6601 taggagtaaa aactatatta ggtgtttcaa atatttcatt tggtcttcca aataggaaag
     6661 cacttaatgc ttcttttcta acattagctt taggggcagg gcttgattta gcaattataa
     6721 atccaaatga atatagtatg atggaagcta tcaattcttt taaaatatta aataacacag
     6781 ataaaggatg tataaactat ataaatcaat atagcaatat aaataattca aaaaaatcgg
     6841 attcatctac aaagattgat aaagatttgc ccttggatat tttagttgaa agaggattaa
     6901 aagatgaagc aaaaaatgta acactgaatc ttttaaaaga gaaggatgaa aattatatat
     6961 tagatgagat tttaattcct gctcttgata aagtaggaaa aagatatgat agtggagata
     7021 tatttttacc acaattgatt caatcagcag aaacggtaaa agtttcatta aatacaataa
     7081 aggaaacttt attaagtaaa agtagcaata atgtatccaa aggaaaaata atagttgcta
     7141 cagtaaaagg tgatatccat gatattggga aaaatatagt aaaaataatg ctagaaaatt
     7201 atggatatga agttatagat ttaggtaaag atgtccctat tgaagaagta gtggaagttg
     7261 ctaaaaagag aaatataaaa ttagtaggat taagtgcact tatgactacg actgttcaaa
     7321 gtatgaaaga tacaattcag gcactgagag ataatgagat aaatgcaaag gtatttgtag
     7381 gaggtgcagt tttgacagaa gaatacgctg aagaaatggg tgctgattat tattcaaagg
     7441 atgcaaagtc tgctgttgaa atagcaaagt taaattttta gcatataaat taaaataaag
     7501 gttttactaa ataggatatt ttatatataa tataaatata agttttagat acttatgttt
     7561 aaaacaatag gaattaatct ataataatga gattaattaa tataaaaaat atttatcatc
     7621 aacattaaaa agggctggga agtctggtat aacttccatc agcccttttt agttaaaatc
     7681 atatgttaaa aagcccaatt accattttta aaaatctgta tttgctttcc atcataagtc
     7741 tcaccaataa tatccatatc agcagaacca atcataaaat ctacatgtgt taaactgtca
     7801 tttacaccat gttcatccaa ttcttcatct tttaaattat ttccaccttc cagacaagtc
     7861 ttataagcag aaccaattgc aaagtggcaa gaagcatttt catcatatag agtattataa
     7921 aaaattatat ccgtattgga tattggtgag ttgtatggaa caagagcgac ttctcctaaa
     7981 taatgagagc cttcatccgt ttcaataagt ttttctagtg tatctagtcc cttttcagca
     8041 gagaaatcta ttattttacc atctttaaat gttaagaaga aattgtcaat tatgtttcca
     8101 ccataaacaa gaggttttgt actataaaca gttccattta ctttaaactt atgtggcatt
     8161 ccaaaaatct cttcagttgg aatattcgga ttaaaactta ttccattagg gtctttggat
     8221 gctccactta gccaaacatg accctttggc aagtctagag tcaaatcagt ttttgaggat
     8281 ttatatttta aagttttaaa gttattttta tttagaaagt ccattttaga tttaagattt
     8341 tcattatgtt cttcccatgc ttttataggg tcctgaccat ctactcttga acatttaaat
     8401 atagcatccc acagtttttc tattgcttta tcttctgata aatttggaaa cacttttttt
     8461 gcccaatctt ttgttggcac agagactata ctccatttac atttatcagt tagagtgtaa
     8521 gaacgccatt cttttaaagc ttttccagat gccttctgaa aatttgcaac tctttcaggg
     8581 tcaacatctt ttaataaatc tggattttgg gcatagactg ataaaaatgc cccaccttct
     8641 tttgctatat ctacatattg ttctgctgtc catttgggga actcatgaaa tgcttcatct
     8701 ggagcatata agtatttaat aagagtactt tcttcatcag accactctat ataaacattt
     8761 ttagcaccac atttatatgc ttctttaagt gcatttcttg caaaatcagc acattctatt
     8821 ggtgacttta taagtagaat ttgacctggc tgtatgttaa ctccaacttt tattgctaat
     8881 tcagcgtatt tttctaaatt tctatttaaa ttcatattta tcctcctata tttttaatat
     8941 tgaataactt ataaagtatt ataacattac ttatgttttt aatattgaac aacttataaa
     9001 atattataac tttacttaga ttttgggtca tattcaaaat atttatactt tatagattga
     9061 gaaataaaaa aaatatttta attaaaacaa attatgagga taacaaactt atttaagcag
     9121 tatgtttttt gttgataata atattaaagt agaaataaaa atactttttt agtgaaaag
//
LOCUS       DAEMIK010000155         9176 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEMIK010000155 DAEMIK010000000
VERSION     DAEMIK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.157"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            247..1458
                     /locus_tag="KPK87_003223"
     CDS             247..1458
                     /locus_tag="KPK87_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2539342.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1651..1911
                     /locus_tag="KPK87_003224"
     CDS             1651..1911
                     /locus_tag="KPK87_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG2539343.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2264..3976
                     /gene="ptsP"
                     /locus_tag="KPK87_003225"
     CDS             2264..3976
                     /gene="ptsP"
                     /locus_tag="KPK87_003225"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG2539344.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            4299..5849
                     /locus_tag="KPK87_003226"
     CDS             4299..5849
                     /locus_tag="KPK87_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2539345.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGNEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            5875..8576
                     /locus_tag="KPK87_003227"
                     /pseudo
     CDS             5875..8576
                     /locus_tag="KPK87_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         3641 a          840 c         1603 g         3092 t
ORIGIN      
        1 tctttaggta tataagcagt tgtaagtatt aaaaaattat aatcttttaa ttgtttattt
       61 aaatttagaa ataaaaagtt agccatataa aaaatattaa aaatatatac ttttcaatat
      121 ttttttgata aaataagaat gaatttgtgc aattttattt ttctccaata actaaaaata
      181 gttttttgaa ttaatatttg cttaagaata aaaatcagaa acgtttttga tatagtaggt
      241 gataatatga aaggattagt tttagaaggt ggaggaacaa aaggagccta ccaaataggt
      301 gcatataaag ctttgagaga gcttggtata gagtttcaag gagtaactgg cacatcaatt
      361 ggtgctttaa atggagcata tatagttcaa aatgatattg aagtaatgga agatatttgg
      421 ttaaattatg actatactca ttttatgaat attgatagag aaacctatga gaggtttaaa
      481 aatgtagatt tcactactaa aaatctcaat gcagtgtttg agcttataaa taaagctaga
      541 aaaaatgaag gtatagatat aagcccatta agacaactaa tggagacaac attaaatgaa
      601 gacataataa ggaaatcaaa aaaagacttt ggacttgtta cggtctggtg ggataaaaaa
      661 attaatccat atccattgta tatcgaagat atacctaaag gaagattgat tgattattta
      721 attgctagtt ccagtttgcc tatttttcaa acgaacatga tggatgataa gttgtatctt
      781 gatggaatgt tttgtgataa catgccaata ggactcttgg aagacaaagg ttatgaagat
      841 gttgttgtaa ttaggttgat tgatgatttt attgggaaaa tgaatctaaa tcgacatcaa
      901 aatataaata taaaaactat aataccatca gaatatctag gaggttctct taataaagat
      961 agagataatg tagaaaaaaa catccgacta ggttacttag atactatgaa agcatatgaa
     1021 agatgcactg gaattaagta ttattttgat gtagagtata aatataatga agaatattgt
     1081 tttgataaat ttagaagtat gagcaatgaa acaatacaat atatctgtaa tttattgaat
     1141 ataaaaagag atgcttcact tagaactgtt atggaaagtg taataccaaa gcttggtgag
     1201 gtgcttggat tgccaaagga tttttcatat aaagatttgt tttactgtat atatgaaaag
     1261 aaacttgaag aaaataatat aaacaggata aaattatatg attttaacaa ggttatagat
     1321 gttgtaaata caaatattga ttctaataga aatttagctt ctaattttga agttagacct
     1381 aaaattagta ttaaaaagaa caaaaacaca aaattattga caaattgtat tataaaagac
     1441 ataaaaaatc aaagttaaat aaaaaacaaa aaaaaatttg taattatact cgaaaaaagt
     1501 gtatattagt agtataatat ttttgtagca ctagtgggaa caaaaaatga ccataggaca
     1561 aaaaagtacc acttagtacc attaaagtaa aaaacaggaa aacaatttta aaaaatataa
     1621 aatgtaaagt ttataggagg atttaaaaaa atggaaaaag tagttagtat caaaaatgca
     1681 agtggattac atgctagacc agcaggaatg tttgttaaga aggcagctga atttaaatca
     1741 acagtagagg ttgtagcaaa agacaaaact gtaaatgcaa aatcaataat gggaataatg
     1801 agtttaggtt tagctcaagg agaagaatta aagttagttg taaatggaga agatgaagaa
     1861 gtagctttag ctgcaatggt tgaattagtt gaaagtggat ttggagaata gatataataa
     1921 tttttaaaaa tttgatattt agaatctaat attacttaaa gtaaagtaac agagaagtat
     1981 taaacatata cagaccaaca tttgtagata tttaatttct ttgttacttt catgtttatt
     2041 taatcagaag attaagacta ttgttaattg ggtataaaaa tataaatatt ttagaaaatg
     2101 aaataagata tttttaatca taaattcaga gtaaaaatac agtaacaaat agtttataaa
     2161 attttataaa aaattataag taaataaaac tataaaaatg taaattattt atataaattt
     2221 ataaagttta tgtgctagat gatatttttt aggaggtagg gttatggctt acaaaggaat
     2281 aggtgcttct cctggtgttg ctttagggaa agcgttagta gttgaacata gtgaattagt
     2341 aattgaaaaa aaatctatag ataatgttga agctgaaatt gctaaattag aagatgcagt
     2401 tgcagtttct aaagaagaat tagtaaaagt taaggaaaag gcttctgaag aattaggagc
     2461 agaggaagca gaaatatttg aagcacattt attagtttta gaagacccag agttaattgg
     2521 ttctgcaata gataaaatta aaactgaaag tgtaaatgca gagtatgctt taaatgaaat
     2581 aaaagagatg tttgttagca tgtttgagtc tatggacaat gagtacatga aagaaagagc
     2641 ggcagatata aaagatgtta caaatagaat acttagacat atattaggaa ttaaagttgt
     2701 tgatttatct gctttaagcg aagaagttgt attaatagca catgatttaa ctccatcaga
     2761 cacagcaact atgaataaaa aaatggtttt aggattttta actgatatag gtggaagaac
     2821 ttctcatact gctatcatgt ctagaacttt agaaatagct gcaatcgttg gattaaatga
     2881 tataacttca aaagttaagg atggagattt tgtggttttc aatggagaca caggtgaagt
     2941 aatcgtgaat cctgatgaag agactataaa taaatatact gaattaaaag ctaagtatga
     3001 agatgagaga aaagcattac agctattaaa aggaaaacca tctgtaactt tagatggaaa
     3061 acatgtggaa ttagctggta acataggaac acctaacgat atagaaggtc taataaagaa
     3121 tgatgctgaa ggtgttggtc tttatagaac agaattttta tacatggata gagattcttt
     3181 cccaacagaa gaaatacaat atgaagcata caaagctgta ttagaaggaa tggatggaaa
     3241 gcctatagtt ataagaacat tggatatagg tggagataaa gaattaagtt atttaagtat
     3301 ggaacctgaa atgaatccat tcttaggtta cagagcaata agactttgcc tagatagaaa
     3361 agatatattt aagactcaat tgagagcttt atatagagct agtgttcatg gaagattaag
     3421 aatcatgttc ccaatgattt catctttaga agagttgtta caagctaaag aagttgtaaa
     3481 agaagtttta gcagaacttg attctgaagg tgtggcttat gctaaagatg tagaaattgg
     3541 tatgatgata gaagttccat ctgctgctgt gatatctgat gtattggcta aacacgtaga
     3601 tttcttctct ataggaacta atgatttaat tcaatacact tgtgcagttg atagaatgaa
     3661 tcaaaagata agttatcttt acaatcaatt taatccagct gttttaagac ttataaagac
     3721 tgttatagac aatgcacata aagaaggcaa atgggcaggt atgtgtggag aatctgctgg
     3781 agaccaaaaa atgataccaa tacttttagg tatgggatta gacgagttct ctatgtctcc
     3841 aatatcaata cttcctgcta gaaagttaat aacttcagtt aaagagtcag atatgaaaaa
     3901 attagctgat gatgtactta atatgggtac tgctgaagaa ataaaaagct atatagaaaa
     3961 gacttttaat atataattaa tgttatatag ttactaaaat tcatttttct aaataacaaa
     4021 attatatttt acgaataaaa aaactatagt atatcatata tactatagtt tttttgttat
     4081 ataaattacg tatcaaaatt tgtaaaatga atagatataa ttactaaata tgtgatagta
     4141 ttggctataa taggatataa aaaataatat tgtaataaaa agtttatatt tagtaatgaa
     4201 aatttaacgt tttacttgaa attatgtgtt ttgtaatgta ttattaaggt tctaatagga
     4261 acattttcat caatttttct atagtggggg taggaattat gaggtacaaa aaaagaaata
     4321 tagataagtc aagtgaactt tcatttcaat ctctagcaaa atattttata actttctata
     4381 tagtaattat attaattatt ctttctatgt tttgtataaa tacaatatgg gttatgaaaa
     4441 aagataatca agatttatta tctacaacat caaatcgtat tgaagatagg gtaggagaaa
     4501 caattaagct attgaactct ttgagtaaag ataattacat aatagataat agtgtgaatg
     4561 gcatagataa aagtaaagaa ctttcaatgt atagtgatga atttgggtat atgatgatat
     4621 gttttgttga taagaatata attgtatggg atgaagaagg tggtaatatt agtctagctg
     4681 gtcgtgatta tatgcagaaa ctttttgcaa caaaaaaata tcaagttaca gatagttttg
     4741 cagcaggggc tgatggaaaa actttaaact acactattgc atcacctata ataaagaatg
     4801 gaaatgtaga tggttgtgta tttgcagcaa tttactttga tgagataaag gatatactca
     4861 aacaaggaaa aggtcaacag caacagaatt tcatattatt tggaagtgaa aatcaaataa
     4921 tgaatttaac aagtgatgaa actgcatatg gagagaagta tatgaagtta tcgcagaaaa
     4981 caaaatatct tggaaccaat gcaaaaaaag tagaaaagga tatgtttaat aataaatcag
     5041 gtgattattg ggcttttgaa aatggaaaat tatactttat gtcctataaa ccaattaaga
     5101 acacaaattg gagtttattt tgtaaagtta atttcttaga tatttttgca ttaaatttaa
     5161 ttcctatggg agctattcta tctgttataa ctttagtatt tattattttg atttgtatgg
     5221 agaaaagctt catgaaaaaa cgaatggaag ttattgatat gcttttaggt tctgttcaag
     5281 aattagaaaa acgtatatat caaacagaaa gacctagtga agtagacttt aaagaaatta
     5341 ttcagctcac aagtaaaggt ctgactgatg gattaactgg tgttgtaaca cgtactgtat
     5401 tttttgacca aatttataat cgtgttgaaa tggaggaaaa tgataaatta tctgtaattt
     5461 catttgttga tttagatgat ttgaaatgta ttaacgactt atatggacat gaaattgggg
     5521 atatggtttt gaaaaatgtt ggatatgtac ttcgtgaata tgagataaaa tataatggtt
     5581 taattgggcg ctatggtgga aatgaatttg ttatgttgtt atctggtttt acatcgagag
     5641 aagaggtgga ggatttattg gatgagattg ctattcgctt acatactgaa gtactaacag
     5701 atgatggtat cattcaaact cattgtagtg ttggagctgt agtatgtagt gtaaaagaca
     5761 ataaagatat agataattta ttaaaacagg ctgatgaagc tttatactat gttaaacgta
     5821 atggaaaagg taattatcat ttagtttaat gtatgtggaa ggagtttata tcatatgaaa
     5881 aatgtatcta aaaaattttt tgtatgtact attattgtat taataacatt tttatgtttt
     5941 agtaaatttg ctatttatgt ttttgacaaa caagaaaaga aagaaacaaa agtagtagaa
     6001 aacatgttaa atgtctacaa gaataacatt attttaaatc tgactgaaaa atttaattat
     6061 agcaaagact tgacatcagt tttagtttca aacaattatt ctatagataa attaaaacct
     6121 attgcagatg aaattcttaa aaacaattct tgcatatata tttcagttgt agaaaaatat
     6181 aatgtagctg catttttttc taattctgtc aaaaaagata ttatcaatac tccaatacag
     6241 gaactgaatt attcttattc attagcaaat tctatcaaag gattagtcgt agaaggtcct
     6301 tctaaagacc agttttcaaa tggagatgtg tttttaatta ctaatcctat ttataaaaat
     6361 ggagagtact ggggagaaat tacagtagct ttagatgcca aaaaagttgt agattctttt
     6421 aacttaaata aattaaaaga atctggattt gagtatgaac tttggcgtgt acacctagat
     6481 aatggttcaa aatctacaat agaaatttct actaaagatt ttaattttcc aaatcacatg
     6541 aaattaagtt taaacttccg actgtttgga ctcttagcat tgtgtacaaa aatggatgga
     6601 ataataagaa tatagatttt attgtatttg ttatattatt aattatttcc atattaatag
     6661 gtgtgatgac atttatttac attgaaaaga agtcgattag taaggaatta aatcaacaga
     6721 gtcttaaaaa tttagaaact ggtattttga atagagaagg ttttttaatt aagttacaag
     6781 agcatctgaa tagaaaaaat aatgaaatag tgttgctgta tatatgtatt actaattata
     6841 gtaaatttgc tccagttatg agctttgaaa aaagacaaga ttatcttaca catatagtaa
     6901 aaaatctaga taattacata aaaagttctc attttattgc tcgtataagt gaggaaggtt
     6961 ttgttctagc tttagaagaa aattgtgata aaaatgaatt gcttaacatt gaaaaaggga
     7021 tttccttaga gcttttacga aaaattaaag tagaaaataa acaagtattt ttaacgattg
     7081 gttatggttc atctaggtgt gttgatgata gaaaagatgc agaatctctg ttactttcag
     7141 ctattgaaga ttatgagaga agatttccag caatgtctcc tattcatgat tttactaaaa
     7201 agtgtgaaga tatgcttgat gaaaagaaac agattgtatt tggtgaatat ccagatttag
     7261 aaataaacaa gttgtctaaa gtaatataca aatattataa aaaagtacag cgtattatgt
     7321 ataaagatta taatattgat attggaaata gacgagggta tatgcgtgat gtcgaagtat
     7381 taattgatta taattctgag agaatgttat acttattttt aatagacata tgcaagtttg
     7441 gaaaatttaa tgaactgttt tctattgaaa taggagatgt tattttaaaa gaagttagta
     7501 acaaattaaa aacaattttt tctaattatc tatatcgtat taatggagat gtatttttag
     7561 gcatttcaat agataaagaa tcttacttag atactttaca taaggttaat gaagtattta
     7621 atagttcttt aagtgtagaa gatgtacttt ttgatgtaaa atgtaatatt ggtatttgtc
     7681 aatatccata tcatggtgaa aatccatatg accttctgga aaaggtacaa attgcattgg
     7741 agtatgcaaa aaaggatgtc aaaagtaatg ttaaagttta tgataaggaa ttgacagaag
     7801 agatttacca tgaaaataat attattaagg atttaaaaaa aggtctggaa gaacaaacac
     7861 tagaagtttg gtatcagcca atttttaatg ttagtaaaaa ctgttatata aaagctgagg
     7921 cactaattag attgagaaat aaagagggcg gttattattc tgcctatgaa gttattactt
     7981 atgcagaaaa gcatggactt attaaggatg taggtctata tgtactaaaa aaggcttgtg
     8041 atgtaactac aaaaataaaa gataaaaata tagattttga acaaatgcaa ttaaatttat
     8101 ctgttcagca gattgtacat aagcagtgtg caaatgacat attatcatgt attgagaatt
     8161 gtggtttaga taaaaagtat atttgtgttg aagttacaga aacagtactt atggaaactt
     8221 ttgaaattgt aaatgttact cttgataaac taaaaaaaga aggcattagc atagctttag
     8281 atgagtttgg ttctggattt agtagtataa attatctggc aaatttacct attgaaaatt
     8341 taaaaataga taaggaatta gtagaacaaa ttacctattc taaaaaacaa tttgaattat
     8401 taaaaactat agttcaaatt gctaagatta atggcatgga tattgtagca gaaggtgttg
     8461 aaacaaaaga aactctggat ttagttgtaa cttcaggtgt gaatttgatt caaggtcatt
     8521 atttttcaga acctttaagt gaagataaat ttattgaact tatgattgaa gaataaaggt
     8581 taataaaata aagatttaag gtattgaaaa ataattaaaa gtatttaata atagaattag
     8641 ggaagaataa gtttataatt aaaaataatt gattagtagt taataaaata aatgtgatat
     8701 ccaaataaat cctaaatcta atttgagttt taatatagat agtctaaagt ttgattatta
     8761 aagatttaat tgtaagttgt tacagttgat gatattgatt agagattaaa gtttagagta
     8821 gaaagttacc ttaaatttaa aaatgcaaga taacccaatc taaaaattat aatcactcaa
     8881 taggtttaag gatttatttg tttattataa ataggagggt gtctcaaaat gaacttttta
     8941 gtttatgagg cactctcttt tatatagaat taaatatatt ttattatttc aacaatgaaa
     9001 aagaagctta tctctatttt gagacagcct ctttgtatac tattattaat ttaatcaata
     9061 acataattta aaattatatt attagttaag atttcctgct ttattagtaa ataattcttt
     9121 aatagcattg tttctggaaa ctaaatcgtt atcagagttg tttgtttttt tcataa
//
LOCUS       DAEMIK010000156         9032 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEMIK010000156 DAEMIK010000000
VERSION     DAEMIK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.158"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            175..516
                     /locus_tag="KPK87_003228"
     CDS             175..516
                     /locus_tag="KPK87_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG2539346.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            667..1080
                     /locus_tag="KPK87_003229"
     CDS             667..1080
                     /locus_tag="KPK87_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG2539347.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1282..1923
                     /locus_tag="KPK87_003230"
     CDS             1282..1923
                     /locus_tag="KPK87_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG2539348.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1947..3200
                     /locus_tag="KPK87_003231"
     CDS             1947..3200
                     /locus_tag="KPK87_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2539349.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3246..3896
                     /locus_tag="KPK87_003232"
     CDS             3246..3896
                     /locus_tag="KPK87_003232"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG2539350.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNAKEVIQTAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3947..4543
                     /locus_tag="KPK87_003233"
     CDS             3947..4543
                     /locus_tag="KPK87_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG2539351.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4556..5395
                     /locus_tag="KPK87_003234"
     CDS             4556..5395
                     /locus_tag="KPK87_003234"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG2539352.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5640..6794
                     /locus_tag="KPK87_003235"
     CDS             5640..6794
                     /locus_tag="KPK87_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG2539353.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDRYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7100..8797
                     /gene="ade"
                     /locus_tag="KPK87_003236"
     CDS             7100..8797
                     /gene="ade"
                     /locus_tag="KPK87_003236"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG2539354.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKLYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3704 a          871 c         1581 g         2876 t
ORIGIN      
        1 ttaaaaaaag tctttttaac tttaaaaatt aatgttaaaa aataaactta tacattaata
       61 ttaataataa ttatttaaat atcttaagta aatcagtctt acatatattt ccgatagatt
      121 atattgataa aaaacactaa aattaaatta ataaaagtac ttggagggat ggaaatgaaa
      181 gaggaaaatt ttattaatgt tgaatacgag gataaacgtt tttcaattaa tagtaaagaa
      241 gttttgtaca tagaaaagca agagtatgaa gtaaagattt atactgaaag tggtaattat
      301 gaagttggag aaaatgctcc ttgtataaga aaactattga aagaagaaaa ttttttcaaa
      361 tgcagtaata gttatattgt aaatctagac aagtgtgaat tttatctcag caataattgt
      421 atttttgtga ataataattt tataagaatt ttaggttcag aaagaaaaaa gaaattaaaa
      481 aagaaattag aaaaattata tgattatgaa gattaatatt gagaggctgt attttataca
      541 gcctctttat ttattgattg tattattaat aaattttaaa ttatgataaa atataaaaca
      601 agtaacttat ttcagaataa aatacttttt aaaagaaagg gacgagatat gggagattat
      661 aataaaatgc ttgaattaaa gaataactta ggtatcagtg aaagaaagtt aaaatatcct
      721 tgtatctata aacatttcaa agggaaatat tatgctacaa tgggtcttag caaggctatt
      781 gatgatatag aaaacatatg tgaaatatat ggtaaagaaa atttaatcca aaacagaaat
      841 aaatataaat tagttattag acatactgaa agagaagaag atatctatgt ttatagggat
      901 ttagatggaa acttttatca taaaaaagaa gaagatacta atgatttagt tttatacaag
      961 accttatatg atgatacagg aatatttgca agacctttgg atatgttttt agaaaaagta
     1021 gatacagata agtatgtcaa ttcaattcaa gaatacagat ttgaagaagt atataaataa
     1081 tataagagaa atatgtttat aaatttagaa aaattcttta acagtacaat aatttgcaga
     1141 aatacaaatg agatttattg taaataatat gttagattag attataaaat agaaacaaag
     1201 taaagtatta tatgatataa ttaagttttg agaagtgata atttatattt aataaaaaat
     1261 aggcatatat taggaggaca tatgggaaat actttttata tagtaagaca tggacaaacg
     1321 gattggaata tattgggaaa aacacaaggt catggtaatt ctgaccttac gcctcaaggt
     1381 atagaacagg caaaagaact ttctgaagat attggcaagt attcaattga ttatattttt
     1441 agtagcgacc ttgggagagc aatgcaaaca gctcaaattt taggagataa gctcaatatt
     1501 gaagttcaaa aaactgaggc acttagagaa atgggattcg gagtttggga aggcctttta
     1561 ataaaagaaa ttcaaaaaga ttattcagat atatatgcaa catggagaaa tgaaccacac
     1621 ttagtaaata taccagaagg cgaaactctt aagattataa aggagagagt agatgctttt
     1681 ataaaggaac taaatgaaaa atacgataat aaaaatataa ttcttgttac acattcaatt
     1741 acattgaggg ttatgttgtt gtcattttta gaatcaggta tggaaaatat atatagaatt
     1801 aagcaagata atactgcttt aaatatagta gaatttaaag attatggacc tgttatagta
     1861 aaaatgaatg atacaagtca tataaaaaat catgtaaaga taaataattc tgcactggag
     1921 taggaatcaa ttataaggag aaatttatga gtaaagttat agtagttggt ggaggagctt
     1981 ctggaatgat ggcagctttg tcagcttcaa aaaacaataa tgaagtaata ttagttgaga
     2041 gaaatggaga attaggaaga aaacttaggg caactggtgg cggtagatgc aattttacca
     2101 ataatagaga gatagaagat ttttttgata aagttgtcag taataaaaaa tttctctata
     2161 gtagtttcta tacatttaca aataaagacc ttatttcgta ttttgaaagt agaaatttgg
     2221 aatataagat tgaagaagaa aatgaccaca aagtatacac taaaaatgat aaatctatag
     2281 aagtaataga agtattaaat aaagatttac tcaatcacaa tgtaaagata atgtataata
     2341 agaaagttat agatattata actgaagaaa ttgctctaaa agatgattct aataaggaca
     2401 agtcaaagta tctaataaaa gggattattt tagataatgg agataaaata ctaggagaca
     2461 aggttattat atcaactgga ggagtgagtt attcaaagac gggttcagat ggttcaatgt
     2521 ataagattct taaaaagcat ggacatacat taaacaaact atatcctgct ttagttccac
     2581 ttacaataga ggaaaaatgg attaaagatt tacaaggaat atccatgaag aatgtggaaa
     2641 tatcttgtaa gattaagaaa aggaaaatat caaaatctgg agacatgcta tttgcccatt
     2701 ttgggataac aggaccttgt gttcttataa tgtcttctta tattaacaag attattgaaa
     2761 aggaaaaggt agaattaaat atagatttct taccaaattt aagtactgat gaaatctcta
     2821 gtataatcag agcatttcca aataaaaatg ttctaaataa tcttaagcaa atactacctc
     2881 aaaatttctt aaaagagatt ttttctctat tatctttagt tgataaaaag gcaagtgatt
     2941 tgtctaaggc agatgaaatt agaattattg agtatataaa aaatatgaag ctaacttgta
     3001 atgggactac aggtataaac actggtatgg ttacttctgg tggtatttca gttaaagaaa
     3061 ttaattcttc tactatggaa tctaaattag ttaagaactt attttttaca ggtgaagtaa
     3121 ttgatataga tgcagaaact ggtggatata atttacaaat agcattttca actggatatc
     3181 ttgcgggtat aagtgtataa atataatttt ttaataatta aatatgaata aatagaggtg
     3241 tatatatggg taatttagtt atagctgtag atggtccagc aggagctgga aaaagtacaa
     3301 tagccaagat agtagcaaaa aagctaaata taaattatat agatacaggt gctatgtaca
     3361 gagcagttac ttataagtgt ttaaaaagtg gtattgatgt caataatgca aaagaagtta
     3421 ttcaaacagc tgaaaattcg gatatagatt ttaaagataa caatatatat ttggacaaag
     3481 aagttataaa tgaagaaata agaacaatag aagtaagcaa taatgtttct aatgtagcaa
     3541 aaataaaaga agtaagacag ttgatggttg aagttcaacg aaaaatagga atgaaaagtt
     3601 cagtaatact agatggtaga gatataggtt catatgtttt cccagatgca gattacaaat
     3661 tctttttagt agctactcca gaagaaagag gaaatagacg ctataaagaa ctttgtaata
     3721 aaggatataa tactacttta gaagaagtaa ttgaggatat aataagacga gatgaaattg
     3781 attcaaatag agaatttgca ccattggtaa aagctaatga tgcactagaa atagatacta
     3841 caggaaaaac tatagaagaa gttgtagaag aagttgtatc taaaataaat ctttagtttt
     3901 ttatgcatca taatgaaaat aaatgtagtg ttgaagggag ataaacgtga atttttatag
     3961 atttgtaata aatattttta agggtttttc taaaatattt tttaaatatg aagttatagg
     4021 tgcagaaaat attccagata gagggaatat agtaatagca tcaaatcata aatcgaatct
     4081 tgaccctata ttcttggctg ctgccataga aaatagagaa atagctgcaa ttgcaaagaa
     4141 agaattgttt aaagtaaaac cattgggatt tatattaaaa aaattgcatg ttatgcctat
     4201 aaatagagaa aaaccagatg tatctacaat aaaaactatt ttaaggtcag ttagagatgg
     4261 ttatgtatta ggaatatttc cagagggtac tagaataaaa ggagattctt ttggtaaggc
     4321 taaggcaggt ctttctgtat ttacaataaa aagtaaatct aaagttgttc cagtatcaat
     4381 aatatctaaa tataaacttt ttagtaaagt aattgtatat attggagaac caatatcatt
     4441 tgaagaacat tttaaggaaa aattgagtaa tgatgaccat gaaaggatat cacaagagat
     4501 tttagaagtg ataaaacaaa attactttaa atattcaaaa taagggggta aagttatgaa
     4561 cataaaaata gcaaaaaatg ctggcttttg ttttggagta aaaagagcaa tgaaaatggc
     4621 atgggatgaa gttgaaaaaa atgattctgg catatatgct ttaggtcctc ttattcataa
     4681 caagcaagca gtagcaaaat atgaagaaaa aggtcttaag actgtgaatg aaattgatac
     4741 aataccaaat catgaaaata tgataataag gtctcatgga gttcctgaaa atatatataa
     4801 ggaagccaaa gataaaaaat taaaaattgt ggatactaca tgtccatttg taaagaaaat
     4861 acatacagta gtaagtgaat atcataataa aggctatgaa ataatagtta ttggagatat
     4921 gaagcatcca gaagttatag gtataaatgg atggtgtgaa aattcagcta taataataaa
     4981 gaccttagaa caaatggaaa atatggaatt tgataactcc aaaagatatt gtcttgttgc
     5041 acagactaca ataaatccag agttgtatat aagtatagtt aataaactat cagataaatt
     5101 agaagaaata gtatttaatg atacaatttg ttcagctaca aaaacaagac aagaatcagc
     5161 taaagagtta gctaaagaag ttgattgtat gatagttata ggaggtaaac acagttcaaa
     5221 tactcaaaag ttagtaaagg tttgtgaaga cttagtacca acttttgcaa tagagactaa
     5281 agatgaattg gatgtaaata cgcttaaaaa atataaaaat ttgggtatta cagcaggggc
     5341 atctacacct aattggataa tagaagaagt tgtaacattc cttgaaaatt tataatttga
     5401 taggtaataa aaataggtgg acattgttat aatttatcat aatatccacc tttgatttca
     5461 tattacatgt ggtaaataga taacctaata tcaaaaagtg tgtatcttaa gatgaaaaac
     5521 taatagtata agatgcgcac tttttaatat tagaaattca aaagaaatgt tgatattgat
     5581 tatcaataga aaataaaata taatataatt gttgcaaaat tagtctagga ggtaaattaa
     5641 tggaaatgtt gatatccgct tctgaagagg cgtttttaca tgtaggttct atgattggat
     5701 tttttatttt gttatttgga tatattaatt ataaaactag tggaaatttt actaatatca
     5761 tatcaaaaaa cagaaaattt caacccttaa taggtgcttt aattggagct ataccaggtt
     5821 gtggcggaag tttggcaata atgccattgt atataaatgg aaaattaagc tttggagcaa
     5881 taattgcaag tttaatagct agtatgggag atgcagcatt tgtacttatt tcatcaaata
     5941 ttaaaatgta tttttttgtc actatagtta gtacaattac aggaataata actggacaat
     6001 tagtggatta ctttaaacta gaagaaaaat tagggttaaa aaaccgtaga aaatcagata
     6061 gatatgatat aagtaaaaat aaccctaaag aaaataaaga acaacataat catgatgaaa
     6121 taattttaga cactctagct aaatctcatg gaaatacaaa tagacttgca tttataataa
     6181 cacatggaaa gggatataaa atttatatag gaattatagt aatcggtttt atatttatgt
     6241 cacttgctca ctctggactt aatcttccta taattgagaa gttacattca ttggaagaag
     6301 taatagcagt gattggaata ttattttcaa taatttatat gtggtgtttt aaaaaggtat
     6361 ttaaaaattc taatcaaaaa gaggaagaaa ataaaaagat ttctttgcga gaaatgctta
     6421 tccattctgt tggagaaata tcatttgtaa ttacatggat ttttattgca tatcttattt
     6481 acgatttgat aatattaatt ttaggtggag atgaatactt agttaaatta gtattatcaa
     6541 caggtgtaat aagtgtattt ataggtgcag gtcttggact tattccaggt tgtgggatac
     6601 aaattgtgct tatgtcattt tatttgaagg gaactatacc acttggtgca gtgatagcca
     6661 actctatttc tcaagatgga gatgcacttt tcccactttt agcaatggat aaaaaatctt
     6721 ctttatgggc tatgattatt actactatac cagcaatact tgtaggtatg atagtttatg
     6781 catttttagg ttaaatataa ttatgaataa agaagtatta aaaaaataat aaagcacata
     6841 aatttattta taagaatagt gaagattaaa tataaaaata aaaaattcaa tatgaattaa
     6901 attattgctt aaatattaat gagaacatgg gagataatat tattcctcat gttctctcta
     6961 tttaattaaa ggaattatgg aacaaatttc aagtgatact ttctatcaaa actcatatat
     7021 aatatatatt aataaattaa actataatac gtaatacagc cagtatattg atataaaaaa
     7081 cttaggagga gttgtaaaaa tgggtaattt aaaaagacag tgtgatgttt caagtggaag
     7141 agaaaaagct gacattgtac taaaaaatgg gacaatcata aatgtattta cagaagagtt
     7201 aataacagga gatgtagcaa tagtaggaga cactatcgtt ggcattggcg attacaaggg
     7261 caatgtagaa attgattgta gcaataaata tatatcacct ggctttattg atgcacatat
     7321 gcatattgaa tctacaatgg taatgccaat tgaattatcg aaaaaattat taaaatcagg
     7381 tacgacaact atcatagccg acccacatga gttggtcaat gtaaaaggag ctagtgcaat
     7441 agatttttta ttagaatcga caaaagatat accacttaat gtatatatta tggtgccttc
     7501 atccgtacct gcaacaagtt ttgaaactaa tggagttggt aaatttagtg caaaagacat
     7561 ggagagttat gtaaataatc ctagaatttt aggtttagga gaggtaatgt gttttaatga
     7621 tgttataaat tctgaaaatg aaatcttgga taaactagag ctttttaaaa ataaagttgt
     7681 agatggacat gcacctaata taaatggtaa atcacttcag acatatgtat gtgcaggaat
     7741 agaaaatgac catgaatgta taacttttga tgaagtatat gaaaaattga gagcaggatt
     7801 aaaaatttta ataagagaag gaagcgcagc aaaaaattta aagtctatag taagtggtat
     7861 gctaaagcat aacttgccaa ttgaagaatt tatgttttgc acagatgata agcacttaga
     7921 tgatatagaa aaacaaggtc acataagatg gaatataaaa tgtgcgattg atttaggtat
     7981 ggaacctgtt agggctataa aagttgcaac ttataattct gcaaaagcat atgggttaag
     8041 gaaaattgga gcaataggtg cgggttataa ggcagatatt gttgtattga atgatttaga
     8101 caaaatggaa gtggatagtg tatataaaga tggtaatttg gtaaacgaag agatgttttc
     8161 taattacaat tatgagatta aagataaaga attgcttaat acagtcaagt ttaaatatat
     8221 taataaagaa aaaatacagt taaaagtaag tgaaaaaaat tatgtaatgg agattgtacc
     8281 ttatcaaatt ttgacaaata agttatatga atctttacca tgtgcagatg gatattttgt
     8341 tccaaataaa gagtattcaa aactttgtgt tgttgagaga catagaatga ctggaaatgt
     8401 agcaattgct ccactaaaag gatttggcat aaaaaatgga gcaattgcta ctactgtggc
     8461 ccatgattct cataatatta tagttgcagg ggataatgat gatgatattc tagtagctat
     8521 aaattactta aaggaaatac aaggtggtta tgtaattgta tcaaatggaa aagttcttgc
     8581 acacctaagt cttcaagtag ctggattaat tagtactttt acagctgaag aagtacaaga
     8641 aattactgat aatatgttgg agatagcaag aaaaatggaa gttccagagt atgtagaccc
     8701 ttttattaca ctttctttta tggcacttcc agttgttcca caaataagac ttacagattt
     8761 aggtctattt gatgttgagg aatttaaatt tatatagata gtataaaagt aataataaat
     8821 gaggctttct caaaatagag ataagtctct ttttattgtt gaaatgatga aaatatattt
     8881 gatttcatac aaaaaagagc atctcatgaa ctaaaaattt tatttttaga tatccttatt
     8941 ttaattaatc tatcatttaa cattgtttta agcatagata tcttaataaa actattttca
     9001 gtctatttta acatataaat acatgaaaat ta
//
LOCUS       DAEMIK010000157         8837 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEMIK010000157 DAEMIK010000000
VERSION     DAEMIK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.159"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            305..457
                     /locus_tag="KPK87_003237"
     CDS             305..457
                     /locus_tag="KPK87_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539355.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            651..1574
                     /locus_tag="KPK87_003238"
     CDS             651..1574
                     /locus_tag="KPK87_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG2539356.1"
                     /translation="MVGAFVLPNYAITLSEMSNLWGNFTMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMALVSVLFFTYSIYLCATWKDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VEKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   1841..2456
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1841..1869
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            2647..2799
                     /locus_tag="KPK87_003239"
     CDS             2647..2799
                     /locus_tag="KPK87_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539357.1"
                     /translation="MKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            2919..3732
                     /locus_tag="KPK87_003240"
                     /pseudo
     CDS             2919..3732
                     /locus_tag="KPK87_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            3784..3945
                     /locus_tag="KPK87_003241"
     CDS             3784..3945
                     /locus_tag="KPK87_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539358.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(4273..4737)
                     /locus_tag="KPK87_003242"
     CDS             complement(4273..4737)
                     /locus_tag="KPK87_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539359.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(4873..5190)
                     /locus_tag="KPK87_003243"
     CDS             complement(4873..5190)
                     /locus_tag="KPK87_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539360.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(5473..5769)
                     /locus_tag="KPK87_003244"
     CDS             complement(5473..5769)
                     /locus_tag="KPK87_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539361.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(5788..6201)
                     /locus_tag="KPK87_003245"
     CDS             complement(5788..6201)
                     /locus_tag="KPK87_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539362.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(6185..6418)
                     /locus_tag="KPK87_003246"
     CDS             complement(6185..6418)
                     /locus_tag="KPK87_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539363.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            6637..7011
                     /locus_tag="KPK87_003247"
     CDS             6637..7011
                     /locus_tag="KPK87_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2539364.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            7301..7819
                     /locus_tag="KPK87_003248"
                     /pseudo
     CDS             7301..7819
                     /locus_tag="KPK87_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8255..8509
                     /locus_tag="KPK87_003249"
     CDS             8255..8509
                     /locus_tag="KPK87_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539365.1"
                     /translation="MGNKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            8545..>8837
                     /locus_tag="KPK87_003250"
     CDS             8545..>8837
                     /locus_tag="KPK87_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539366.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRV"
BASE COUNT         3577 a          945 c         1383 g         2932 t
ORIGIN      
        1 aaaaaaaata cttattgatt atattattta attattttat gtagatattc ttaggttggt
       61 atggaatttg tactaaccta ttaagtaatc aataaaacta aataaacata gaaagcactt
      121 acaaatatgt aggtgctttt tattgaaaaa atagaaaata aaataaaaag taagatatag
      181 gaaaaatatg taagaattat atattataat aattgtagca agaagatgta atctacaatt
      241 tatagagtgg agttcatgta ttaaaaaatt atcctcccaa cgataagaag ggaggtgaat
      301 atacatggat aattttttat ttaatgtgtt agctagtgta acagctagtt acatagttta
      361 tttgattagc aagttaatca aaaaagtaaa aagccactct accgcaaaga gtgacttaaa
      421 gattgatata aattttaaat atcatcgtaa aaaataactt tcagttatag aacttcactc
      481 taccgcaaat agattgtagt tcttcttgct tttattatac cacaaattag aaaaaatatt
      541 gcatataata ttttttatag tcaataaaaa tatgaaattt tttatacaca atgatgatga
      601 atctaactag atgaataaat ttaattgtaa ataagaaagt acttacttaa atggtaggtg
      661 cttttgtttt gccaaattat gctataacat tgagtgagat gagtaatttg tgggggaatt
      721 ttacaatgtg gatattagta ccaataataa caatagtatt acttataata gctgtttctt
      781 ctatgcaata tattttagtt atgatagctt ttttgttgat tatatattct tttatagaaa
      841 agaaaatagt tatggctctt gtgtcagttc tttttttcac ttattcaatt tatctttgtg
      901 caacttggaa agataaatcc ttgatagcag ataataaagt tgagactgtt aaggcacaaa
      961 gagaaactgt agaaagagaa aaagaaatgg aaagaagaag aatacaagaa gaagtagaca
     1021 aagaaagata tattgaaaaa catggaatgg aaatatcaga aaatgattta aaagttaagc
     1081 tagaggcttt agtacctcaa gaatataagg ggaagaaata tgaattaaaa gtagaaaaat
     1141 ttaaaagata tagtatgtat tttgatttaa ctgtacaaaa tgaaaaattt agtaattctg
     1201 aagaatgtaa aaaatttgta aaagaaattg ctaatgatct taaaaaaatt aaaataagca
     1261 aggcgtactt taagtttcat tcaaaagatg acggcggtat atataattct gtttatattg
     1321 attattttag aaacatacaa aataatgtag ataatgttga aaatttagaa ttcaatgagt
     1381 ttgaacttaa aaccgaagaa gaagagaagc gagaacaaga gaaaatagaa caagaaaaaa
     1441 acagttataa taactacatt caaaacagag tggttgaccc actagataga ataaagaaac
     1501 taaaagagtt gctagattca ggagcaatta cacaggaaga atataataaa aagaaaaaag
     1561 aattattaga atagataata tagtaagcac ttacaagtat gtaggtgctt ttaaatttac
     1621 aaagtattcc attttaattt tatagtttag attttatgat ataataaaaa tatagaagtt
     1681 ttgcagtgtg cgatatttgt tacaaagtag ggcttaatac ttgaaatcta agatgttgag
     1741 ggtgcgtgat aagtgttatc aattgcacta ttgcccgctc actgcaattt taagagtatt
     1801 gtatatatgt aggtattgga aatgctaagt ttattttggg gttttagatt aactatatgg
     1861 aatgtaaata gttttgaaga gcaaacaata gatttttacg actcatgttt tagattaact
     1921 atatggaatg taaattaaca ctctctactt gcaaatataa atataaactg gttttagatt
     1981 aactatatgg aatgtaaatc tatttcttct atatcctcta tttctaagtc attgcgtttt
     2041 agattaacta tatggaatgt aaataaattt tactctgaaa aaaattcagg tggtgaaaca
     2101 agttttagat taactatatg gaatgtaaat ttagaatcaa aagcacttaa tcctagttct
     2161 ttcatagttt tagattaact atatggaatg taaatagctt tttagcttcg tctactactt
     2221 tataagcaaa gcgttttaga ttaactatat ggaatgtaaa tgatgaacaa taaaacaatc
     2281 atctaaagac gaagaaagtt ttagattaac tatattgaat gtaaatgttc ctgttgtgtt
     2341 ttttactaat cccattttat atgttttaga ttaactatat ggaatgtaaa ttgctgtgat
     2401 ttcactaata aaacaattaa taattgtgtt ttagattaac tatgtggatt caaaattaaa
     2461 taaacaaaga aagcacttac aaatatgtag gtgcttttat tctgctcaaa attggtcggt
     2521 tgggtaaaat cattagaaaa aattagtaaa aacctctttt ctgtaactcg ttacaatatt
     2581 attattaatg taacgagtta cagaaaagag gtgaataaaa tagcaactaa aagtagggca
     2641 gagtatatga aaaattgtcg aaaagataaa agaggtttta gtgtactttt agacaaagaa
     2701 aagttagata aatttgatga agtattagag gaaaagaatc taaccaagaa agaatggcta
     2761 gaagaaaaaa tcgacgagga actggaacaa aaggaataaa aaataagggt cactcccacc
     2821 gaccaaagtt tgagtaaccc ctatgatgta tactatcgta tatcaattat aatatatgtc
     2881 attccttaaa aaaatcaatt attaaggagt gtaatattat gaaaaatgaa ttaatgatgt
     2941 ttgaaggaaa agagattgaa gtatttgaat ttgaaggaag aattttattc aactcaaaag
     3001 atgtagcaaa ttgtttagac ataaaaaatg ttaatgagaa tataatactt atgaatgaaa
     3061 agcaagttgt aaagttaaga aattcggata tcagtaatac tgacatccga aaacttaata
     3121 atgctggaga aaattttctt actgaaagtg gagtgtataa attaatcttt aaatcaagaa
     3181 aagaagaagc tgagagattc caagactgga taagcgatga agttttacca agcataagac
     3241 aaactggtgc atacataact aataatgcaa tcccgaaaag ttgagagaaa aagcaagtga
     3301 gattgaaaaa ttacagttgc ttataacagt acatctatgt taaaagaatt gctagatgat
     3361 gcaggctttg acaataaatc aaaactatta acagctaaaa cattatataa gaaagcagaa
     3421 attgatttac caatagagat aaacgaagaa gaacattatt ttgatacaaa acaaatagca
     3481 tctaaactga aaatatattc taagagtaat aaaccagctc agatggctgt ttgtgagatt
     3541 attaaaaaga ttgatttaga agatggtgaa gttaaaggtg tttgggaaat taatggttct
     3601 tggactggta ctgtaaataa atatacaaag agtgtaatag ataaggtgag aacttggata
     3661 gaggaaaata atagacctac caagattgca ggtgagaaga agaattatta tgtattttac
     3721 aaaattgagt aaatttatca gttgtattaa ataatttagt ttagttttgg ggggattaat
     3781 acaatgtgtg agaatttact tgataatatg tatattgaaa agagaaaaga agaatataga
     3841 attaaacttt tgaaaataag agaaacagat atagatatat ataataagtt agaaagtata
     3901 gtatataaac tttctgagaa aaaattagag aaaaataata aatagacata taaagcactt
     3961 ggatattcta ctgtttcaag tgcttgtttg gtataaaatg gtataatgga gatagagaat
     4021 tatattaacc aaaaaatgga gaaaatagag aagatgattt agttgacgct ttatcagtag
     4081 gagtacctgt aggtaagaca gaatctctag tacgattagt tggagatgga ataagtgata
     4141 ctcaaaaaag tttaataaat aaaagagaat ttagaaatat aactcaaatt ggtggaaatg
     4201 gaaatgagaa agcatttagt gaagtagaaa acttagttaa atagattaga aggtagcaac
     4261 tagagttggt tgttaccttc tttttttata ctttctctct ctaagtattt tatttttcat
     4321 atcttctaat acatctaatg cagtagtata aaattgtata ttatttctat aaggtatagc
     4381 tattggaaga atgcccttgt taactaaaaa aataagaagt acaataaata aaagtgtcat
     4441 aatcattcta ataaaataat tagtgatatc tgataaatca aatgttaagt taattgttgt
     4501 agaaaataca acagccataa tagatatata caaagcaaaa atggggttac tatttttttg
     4561 taactgtcct ttaagcctag attgctctat ttctatatct tgaatacttt ttatggaatc
     4621 catttcacta taatacttat aaacttttaa ataatatttt ttatacttgt ttaaagcttc
     4681 ttcattatct ttaaggtctt tgtcacttaa ctctgattta acttccccaa aatccataaa
     4741 atccctccta caatgttata taatatactt taatttatag catatttaag aacttatatc
     4801 agcaatttgt cgaacgattt tagctaaaaa tagagtataa tgataggctt attatttata
     4861 agatattatt ttttatatag ttatatattt ctttctaatt agataatata atgctgttgt
     4921 tataatggct actaatatta ttgctattat tgtaatatct aatcttcttg taaataagcc
     4981 tattgctata tactcaagac ctgttacata gcgataaagt acttctagtt taacagtttt
     5041 tttcttatct atatttccat atattgattc taatttatta aaatattttt tactacaaaa
     5101 agcagtttta ggtttaatta aatatattat tgtagttcct attataacta ttattccaat
     5161 aagtataaga ttgatatttc caaaatccat agcagatacc tcctattaaa tttgcttttc
     5221 ttaatcaaag tatatcagta acacaagtac aaagagtatc taaaatatta caaatattaa
     5281 caggatagat aaaactatag taaaaattgt aataataaag cgaacgaaaa ttatcatact
     5341 aaaacataag acatgttata attgtattag atagaagatt aacatacacc aaagcactcc
     5401 ttataataaa gagtgcttat ttttttgaaa ttcatcaata tataaactat caagaacctt
     5461 attcaacaca ccttaaaatc gatttaaata gctctttttg ataacataat ttatatgata
     5521 taataaaaaa atagaagtgt accaccaata cacttctata gctataacta agcattctct
     5581 tgctggggga gtgctttttt catttctcca aacaacatta tttacttcat cagtaattat
     5641 caaatcaatc tcattatctt tcaaaatctc tgttgcagta caaattagat tatctaagtc
     5701 aaaacttcga gttatctcta gttgtttgtt atttacttct ctttttagta tataagtttt
     5761 aaaatacatt tatatcaccc cttgtattta ctcttttttc tctaaaaata tattgataag
     5821 taaatatgca acaaataaaa aaatctcaat gacacatagt gttatagcta taactagaag
     5881 tgattgattt ttccatgcac tataagctat gatgaaaaga aatgtaaaag taaataatgt
     5941 ataattcata attttaacta agatatgact tgttttcatt gttaagtaca agtctctttc
     6001 atctgcacta tcgtgtaatt cttcaacaat gccttttctt gaaaaagcat tacaaaaatt
     6061 aaagattgct attacaatta aaagaaatga tgaaattagg aatcttttat cttttaaaat
     6121 gcaataaata actaaactag cacaacataa catacaaact ataataccca ctataaaact
     6181 tcgtttactc tttactttca tgttttttat cctccatttc tttattttct tttaaacaac
     6241 atagttcttc aatcgttgta ccaaacactt gtgccatacg ataggcaagc ataagagaag
     6301 ggctatattg ctctttttca atagaaataa ttgttctgtt tgatacatgg actaaatcag
     6361 cgagttgttg ctgtgttaga cccatatttg ttcgaaattc tcgtactttt gttttcattc
     6421 cataacctct ttctatatga agctaacttc atgtgaagtt aacttcataa tatcctgaaa
     6481 tgagtaaaat gtcaatctca ttagataagt attataaaac ctaaaagaat aatatggtaa
     6541 aaattggatg gaattattta ccttaattct cttgtttgta aaactttatt gatatagaat
     6601 ttaacatata cataaatcgg caaaggggcg cgaattatga aaattagtaa attaccagaa
     6661 gcggaattaa aagttatgag atatatttgt gaagctaaca aggtattaac ttctagggaa
     6721 ttagttgctg caatggaaga aaaatatgag tggaatgaat caactacatt tacagttttg
     6781 aaaaggttag agcgaaggga gtttttaagt acagagaaaa taggcaaact tacacattat
     6841 aatatactag taaaagagaa aaaatatcta aggtttgaaa caaaggaatt tttgaagaac
     6901 atacataaaa attcaatttc aagtttaatg tcagcgttac atggtgaaga cgacgaagtg
     6961 gatgaagata aattaagtga actcgaagaa tattttaaga atttgaaata agaaaatgac
     7021 aaattgatac ataataaata aaatgaaggt atgaaattat aactttttct accttcattt
     7081 tgtttattat tctgaacatg ttaagaaaat gaaataagtt tgtattttat ttatataaat
     7141 tttaatagaa tatgtatagt atatagtaga tagaatgtat aaaaataaaa actgttcaat
     7201 aagaattaat atacattaat tcagctgaat ccaatcaaaa tacttaaata aaagtaatct
     7261 gaaaataaca taattcaaat caatttattc aaataaacct caatttatct caaatcttaa
     7321 gaagtcatta aacaaacttg ctaaattaca aagagaatta tcaagaaaaa caatcggtag
     7381 tttaaataga aataaagcaa gattaaaagt tgcaagactt caagaacata tagctaatca
     7441 aagaaatgat tttctacaaa aactatctac taaattaata aaagagaacg atattatttg
     7501 catagaagat ttacaagtaa agaatatgat taggaatcgt aaactatctc gctcaatttc
     7561 tgatgtgtca tggtcagagt ttattcgtca attagaatac aaggctaatt ggcatggtag
     7621 acaaattgta aaggtgggta aattctttgc aagttcacaa atatgcaata aatgtgggta
     7681 taaaaacgag gaagtgaagg acttaaatat aagagaatgg atttatccta gttgtaatga
     7741 aactcacgat agagatataa atgcaagtat aaatatatta aaagaaggat taagactaat
     7801 aacaattcaa aataaataac ttatgtaaga accgtagaaa ctacggggat ggcctgtgga
     7861 gaattagtaa gacatatgtt agtatgcaaa attctgtgaa gcaggaaacc tgtgacttta
     7921 gtcatgggag gttcaggcta gtattactat tgtattcaaa agctaaagtg gaaacctagt
     7981 gaatatatga atttagaagt taatgagaga gcattagcag ccgactcaat acttattaaa
     8041 attgaagatg aagaagaagc aatgaaagaa gctgaaagag agaaaaagag aggtagaaga
     8101 aggtagaatt taaagttaaa atagttacat tgctaatata tgaaaatgtt acaattttgg
     8161 aaatagatgg atatatatgt tgtatagtat aatgtagata atatatcacc aagtaaaaaa
     8221 caacaaataa atctaaatta tgaggggtgt atttatggga aataaagata ttaaacttac
     8281 aatatgtata tttgcttttg taattggaat aatagtgtta ttttgctgta taggtctagg
     8341 tcaatttaat atggattgta ttataaaaca aaatggtggg tcaatagcta ccgaacaata
     8401 caacatatat ttagaacaat ctatttcaca atatagaaat tttggttcta tactggtttt
     8461 actaggagga ataggaatac tgtttgataa aagtgtcaga aaatactaaa aaagtatatt
     8521 tttatttagg atgtgatgaa tgatatggca agagatgtat ttagtgtaaa gatgacacca
     8581 ctagaagcac agaaaatggt agaaaaaaat ttggaagcag atttagtttc tgttgatatt
     8641 tataacctag aaaataataa gtctataata gttacaatct ttgaaaaata ttatgcaaga
     8701 agcaatagtg atgcaggttt ggtagtgatt tgtgaaaata ctacaggaaa aacacttgtt
     8761 aaaattacct caactggtag tgcaattgga ctatttcaga ttgattgggg aacaggtaat
     8821 aatttgatta aaagagt
//
LOCUS       DAEMIK010000158         8635 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEMIK010000158 DAEMIK010000000
VERSION     DAEMIK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.161"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            222..2081
                     /locus_tag="KPK87_003251"
     CDS             222..2081
                     /locus_tag="KPK87_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2539367.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVGRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2355..2954)
                     /locus_tag="KPK87_003252"
     CDS             complement(2355..2954)
                     /locus_tag="KPK87_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2539368.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3057..3854)
                     /locus_tag="KPK87_003253"
     CDS             complement(3057..3854)
                     /locus_tag="KPK87_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2539369.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3967..4072)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4164..5150)
                     /locus_tag="KPK87_003254"
     CDS             complement(4164..5150)
                     /locus_tag="KPK87_003254"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG2539370.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYISKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5634..7364
                     /locus_tag="KPK87_003255"
     CDS             5634..7364
                     /locus_tag="KPK87_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG2539371.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7474..7554
                     /locus_tag="KPK87_003256"
     tRNA            7474..7554
                     /locus_tag="KPK87_003256"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7508..7510,aa:Leu,seq:tag)
     gene            7889..8404
                     /locus_tag="KPK87_003257"
     CDS             7889..8404
                     /locus_tag="KPK87_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG2539372.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKI
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
BASE COUNT         3121 a         1068 c         1316 g         3130 t
ORIGIN      
        1 aaagtttata aattatatta atagtaataa tctataagaa cttatatatt agaaaaaaat
       61 ttttgtatat atagaggact ctaaagatga actatgtaaa acaaggttta tatattctaa
      121 ttgtatagaa aatttattgt aaacacatgt atattattag aaaaaatagt ttgaaggtat
      181 tcaataaaca taaaaaacaa tataggggat aaaggtgttt tatgaagaga tttttgagga
      241 gaattatctt agttttattt attttgttga tttttataag ttttatttct attaaattaa
      301 ttcataatgt tggagattat ggaaaattga ttaattatgt tgggattgtt cgtggagcta
      361 gccaacgtct aactaaacta gagatgaatc atcaaccaaa tgatgaatta atagaatata
      421 ttgatgaaat cctacaggag ttgattacag ggcatggaga ttatggttta gtaatcactg
      481 attgtaatga atacaatgag gacttacttt tattagaaaa aaagtgggaa gatttaaata
      541 ttgaaattaa aaaagtaaga atgaaagaac agaataacca gttattaagt ataagtgaag
      601 agtttttttc tcttgcaaat gatacagtgt ttaaaataga aaacttttct aaagaaaaaa
      661 gtaattactt aatgacttta ataataatta tatctattat aggtatatta gcatgtatca
      721 tattgatttt acaatatagt aaaaaaatgg ttagactgga aaaattaaat gtagatttaa
      781 aaaatattgc atataaagat gaactaacag gagtaaatac tatagaaaaa tttaaattgg
      841 atgcaaatca aaatatttgt atacataagg ataagaaatt tgctgtattc tatattgatt
      901 ttgaaaactt taaatacatt aatgatatat ttggatatga ttatggtgac atgatactga
      961 aaagatatgc taatcttatg atgaatgaca tagggaaata tgaaatattt gcaagagaaa
     1021 ttgcagatag atttgttgcg ttaagatgct atgtagataa ggaagattta gtgggaagac
     1081 aacgagatgt agacaatgaa ctaattaata taacaaatga aattaaaaat aaacatagta
     1141 ttactgttgt tagtggaatt tgttgtattg aagatgtaaa tgaaaaatta agtatagatg
     1201 gtttaataaa tagagcaaat tttgcacaaa aaactgttaa gaataaacca ggtactaatt
     1261 atgcttttta taatgacagt attcgtaaaa aaatgattga agaaaatact atcaagagta
     1321 gaatacatga agctattgaa aaaagagaat ttatagttta tttacagcct aaagttaatt
     1381 tacatagtca aaaaataaat tgtgcagaag ctttagttcg ttggcttact ccggataaag
     1441 gtattatatc gccagcaata tttattccag tacttgagaa gaattttttt attgccttag
     1501 tagataagta tgtttttgag gaggtttgta aatggattag aaagaggtta gatgaaaata
     1561 aaccttttgt tcagatatca gttaatgttt caagaataca attttataac actaaatttg
     1621 tagaaactta ttccaatata caaaataaat acaggatacc taaaaatact atagaaattg
     1681 aatttacaga gagtgttgct tttgagaatc aaaaacatct tttggaaatt atacatgatt
     1741 tgcatgaaaa tggatttact tgttctttag atgattttgg aaaaggttat tcgtctttaa
     1801 gtgttttaaa agatttacca tttgatgttt taaagcttga ttctatgttt ttcaaggcta
     1861 gtttagataa agataaagag aaaatagtaa taaaaaatat tgtacatatg ttaaaagaac
     1921 ttaatatcac aactgttgct gagggcattg aatgcaagga acaagttgaa tttcttaggg
     1981 atattgggtg tgatttagtt cagggatttg tattttataa accaatgcca atattagaat
     2041 ttgaaaaaat attggataaa gagtttgttt ataatagcta aaatgtactt tataaaatat
     2101 gaactaatta agtaaaatta ttttgtactg aataaatttt tactttattt acagaaaatc
     2161 gcgaaaagtg tattccttta agtgtgggat ggatagcaca aatagaggag aaaaaatatt
     2221 ggccatataa gaatgcataa taatataaat atttgaaagg tcagcatcat ataaaaatta
     2281 aaaagaaaat aaataatttt tctataaaaa agagactatg agaaaattta ctcatagtct
     2341 cctaaataat gtatttattt tcttcctgca acccatttta atccccaatg aaattcagta
     2401 tccatttctc tatgaccttt gaagtattta actacttttt caacactgaa tgtctcatca
     2461 tttacatttt ctatcaatgc tattgaacac atattatagc catttttatg ttcatcaagt
     2521 ttgacaacta agtctgaatc ttgtttttgt ttgattgtta caattccatc agcttcagac
     2581 cagttagcta caccttcgta tataaaagca taaataagta ttctttttat atcttttatt
     2641 ttattaccat ttattcttaa attttctcct tgtgtattac ttcctgttct gtcatctcca
     2701 tctaattgag caaaaggtgg attgtctaaa ctaccaaagg catttcctaa tgcttgaaca
     2761 gcacctttta caccattttt catctcaaat aggcaaccca aatctaaatc tatattactg
     2821 cttcttaaag agccaaaaaa accctttttt tgtacttttt gattccaatt taaattaact
     2881 aagatttcac ctaaatcgct attttccttt tttgtaaggt ttattttttg tccctttttt
     2941 aattctatag ccatagtaat cttcctccac aaacatagat ttatttaaat ggtagcactt
     3001 aataagtcaa tattcaacaa gattagaata ataatattaa aattatacta aatatactaa
     3061 aaagtaggaa tcacaatacc tttgtatttt tcttcaatta agcttcttgc tttatctgat
     3121 gtaagaacat cttgtagaac tttaatcttt tcactgtctt tatcttcagt tctaaatgct
     3181 aaaagatttg catactcgtc atttatagga gctgtataaa ttccgttttt cttggcatca
     3241 agaccagcag gtacagcata agtagtatct ataaatgcag cagtcacatc tggaagagaa
     3301 gatggaattt gagcaatatc taactctttg aattgtattc cctttggatt ttctattatg
     3361 tccttaggac taactaatcc atccccttta ggtaacgtta aaagtccaat actttcaagg
     3421 tatctaaggt tcttagaaca agaagatggg ttattgctta ttgcaattgt atctcctttt
     3481 ttaaattcat caacagattt tatcttataa gagtaaagta ttgctgggca tacatatagt
     3541 tttttaccag ccattatttt gtaacctttg gccttaacag cttcttttag ataaggttca
     3601 tgttgaaata gatttgcatc aatttcacca ttatttaaag cttcgtttgg aagtatgtaa
     3661 tcatcaaagt ttttaacttc taaagtatat cctttttctt taataagtgg ttttaattct
     3721 tctaataact ctcctcctgg aactaaagta gcaccaacaa caattttttt atcatcttta
     3781 gctttagaac atccaaccac agctaaagtt aatactagac ataataacaa acataataat
     3841 tttttgaatt tcatataaat tccctcctga atataattga ttagttaaaa ataaaatacc
     3901 attctttatc acactgcaaa ataaaaataa gcctgagttt aaactcaggc tttaaaagct
     3961 aatttagact catctttcag tataatactg caggatttag caccaatgta tataaaaata
     4021 tactggttgc tgggtttcat agggccagtc ccttcaccac tctggataag aagtatcaat
     4081 ttttaattat gttataaata ttatcatgat tttttacgtc tgtaaacata attttataaa
     4141 tattttaaaa tctttaatgt attttattta acacctaaaa ttccagaaac tagaatatca
     4201 gtatcagcac ctaagaaata gtcatttatt tcaatagaat ccagagtatt tctcaaagca
     4261 ttttcttcat gccttatacc aataagttta ttttctacaa atgcaacatc acattttcca
     4321 aaaaaatctc caaaaaattt tatactagtt attatgcctt tatcaacatt taaattaaac
     4381 tcaacatttc caccagaata ttttaattca tttgaaagag tatattttgg tgaattacca
     4441 aaattccaat cccaagtttc gtattttgat ttagtaagct tttctatttg ttttatatca
     4501 tgctcactca aacaataatt agtattatca gtatttgttt tagaaatata atccattata
     4561 gcatctttaa attctaatat atccattttt gaatgtaaat gttcacttat attggttaca
     4621 cgagatttta cagattttat tccttttcct tcaaatttta taggtttaac ctttaatgct
     4681 tttgagaggt catttatttc agatgaaaat aaaagagtcc catgatgcat tactttgttt
     4741 ttgtagttgt attgagcatt tccagaaaat ttcttgccat ctataagaag gtcatttctt
     4801 ccagaaaatt cggcattgac atctagtgtt ttaagtaaat ctattatggg ctgagtgaat
     4861 cttctgaaat cactaaaatt attattgtta caagctataa atgtaaaatt tatatttccc
     4921 aaatcatgaa atacagctcc accaccagac tgtctcctaa cgacaggtat agaatattct
     4981 tttatatatt ctaaatttat ttcagaaagt gtgttttggt tttttccaac aattatagaa
     5041 ggttcattcc tccataaaat aaataagtct tcatcaaaat tttttagaag atattcttcc
     5101 attgctaaat taaaataagg gtttgttttt tcattataaa ttaataacat taaagtagtt
     5161 cctccataga attgataaaa tttttattat gaatacaatt aattttataa ttcgacaata
     5221 atataaaaac tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt taaataaata
     5281 atatatactc aaaatatgtt taataaaagt gaatagaagg gtagaggcgc aataatctat
     5341 cagtactaat aaggagttaa gcacagagag ttattaggaa aggagatatt gccgaagttt
     5401 tataatcgat gctttaagat tatattgctg ggggtacata gaaaatatgt acaactgtca
     5461 caggtttatt tgtggtgagc tatcatttaa ggaattatat tatatttatt tttactattg
     5521 ttgatatatt gtaatgttta agccttgagt gtttaccact caaggctttt ttagttttat
     5581 tgtaacataa taccctgtac agttcacaaa attaaaagga gagggagatt tatatgagga
     5641 acaaaaaaat taatattatt tttctgacga caatcatgtt cataatgtca acagtaatgg
     5701 tttttgcaga agaagatata gacactattg cactagccaa tgcagaaaaa tttgggattt
     5761 taactttaat accaccatta gttgctataa tacttgcatt tataactaaa aacgtaataa
     5821 tttctttact tataggtatt ctttctggta gttttataat taaagctagt ggtattaatg
     5881 tttttgcgac atttatacag gcatttttag atttagtaga tagagcttta gtatctttag
     5941 cagacccttg gaatgcaggg ataatactac aggttttggc aattggagga gtaataaatc
     6001 ttgttgctaa aatgggagga gctaaagcca tagctgaagc acttgctaaa agagcaaagt
     6061 cagcaaaagg gacacagctt ataacatggt ttttggggct tttagtattc tttgatgatt
     6121 atgccaattc actaatagta ggtcctatga tgagacctgt tgcagataaa atgaaaatat
     6181 cgagagaaaa gctggcattt ataatagatg ccacagcagc accagttgct ggtttagcta
     6241 taatatctac atggataggt cttgaggttg gtttaataca tgatgctttt gaaagtataa
     6301 gtatagatgt agatgcattt ggtatatttt taaatacaat accatttaga ttttacaata
     6361 tattaatatt agctttcatt gtaatatcag cattattatt aaaagaattt ggtccaatga
     6421 gaaaagctga gataaaatct agaagtagaa aaattagtat agaccttgat gaaggcgtag
     6481 aagagttgga tgatttagct ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa
     6541 tcccaatagg aacattgata atagttgcat tagcatcatt ttattatagt ggatatacat
     6601 ctataatggg aggagacgat aaggctctga tacaactatt tacaaattct ccatattctt
     6661 ttgaagcaat aaaagaagca tttagcgcat ctgatgcatc tagagcatta ttccagtctg
     6721 cacttgttgc aagtttagtt gctattataa tggctgtagt aaaaaagatt tttacaatat
     6781 cagaagcaat tgatgtttgg atagatggaa tgaaaagttt agtaatcact ggtgtaatat
     6841 taatacttgc atggtcatta agttcagtaa taaaagagtt aggtacagct aaatttttaa
     6901 tacacctatt atcaggttca ctaccaccat tcttattacc aagtttaata tttggtttag
     6961 gagcaataat atcttttgct actggaacag cttatggaac aatgggtata cttatgccac
     7021 ttgcaattcc attagcatac tcattaaatc ctgatatgtc ttatgtaata gtaagtacta
     7081 gtgcagtttt aacaggtgca atatttggag accattgttc tccaatttca gatacaacaa
     7141 tactttcttc aatgggagct ggctgtaatc atatagacca tgttaacact caaatgccat
     7201 atgctatatt tacagcagta atcactattg tatttgggta tataccagct ggacttggat
     7261 tgccaatata tatagtatta ccagtggcaa tagctgctat atttgtaggt attcaaataa
     7321 ttggtaagaa agttgatgaa gcggaaatag aacttgtaga ataaagatat aaataagttt
     7381 caccttttat tatcactatt ttgttagttt ttagataatt ttaaaaagtt attgaaaata
     7441 atggcgaaat atagtaaaat aaaagagtga taagcggatg tggcggaatt ggcagacgca
     7501 ctagacttag gatctagcgc cattggcgtg ggggttcgac tcccttcatc cgcactttac
     7561 agtacttaat tgtactgaaa aaaactgggg ttgctggctt tcggagtggg ctatcccgtt
     7621 tttatttttt tgtatatata atagattggc actttagtat aaggtgcttt ttttataggc
     7681 aaaaacagta catttatttc tgttttatta tcattcatct attagaatat actttagtta
     7741 agaatgtgtt aataagtagt gttcacattc ttgataaaat gtggaataat atgtattttt
     7801 actaagatgg aaatgaaata attaaaccat taaataatat tataagatga tgaataagta
     7861 gataaatcaa attttgtagg aggatattat ggaaaaatat atatggttgt ttccattact
     7921 ttttattttt catgatatgg aagagataat aggttttgga atatggctta aaaaaaataa
     7981 atcaatgctt gataagaagt atccatttat cagtaaaatt tatgaaaatt atagtaccga
     8041 aggcatggca tttgctgtgt ttgaagaatt tattttatgc attattttct gtatacttac
     8101 agttataaca gagaatcagt atgtatatct actatggtta ggtagtttta tagcatatac
     8161 cttacatttg gtaattcata tagggcagtc aatcataata agaaaatata taccttcact
     8221 tattaccagt atcatatgct taccaataag catttggtgt ataagtaaga gtatttatat
     8281 agttgattgt gaaatgagta caacaattct gtacagtata attggcatta taattgtagc
     8341 attgaattta aagtttgcac agtcgctgat tggaaaattt acaaagtgga tgtcaaacat
     8401 ataaatttta gtttattgga cagttcaagg aatcagaaat tggaatttta tagtattaat
     8461 attttatagt attaatataa tgtataaaat tttatagcaa tgtatttcat aagtataaag
     8521 tgactagaat tttaagaaat atacaaattc tataaaaact aaataatttc aagtatattt
     8581 taaaaatctt atttttcaca agtttaaaat tgtaaacgat ttcatcaagt ctatg
//
LOCUS       DAEMIK010000159         8565 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEMIK010000159 DAEMIK010000000
VERSION     DAEMIK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.162"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            299..1312
                     /gene="aroF"
                     /locus_tag="KPK87_003258"
     CDS             299..1312
                     /gene="aroF"
                     /locus_tag="KPK87_003258"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG2539373.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1334..2398
                     /locus_tag="KPK87_003259"
     CDS             1334..2398
                     /locus_tag="KPK87_003259"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG2539374.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2398..3711
                     /gene="aroA"
                     /locus_tag="KPK87_003260"
     CDS             2398..3711
                     /gene="aroA"
                     /locus_tag="KPK87_003260"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG2539375.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3689..4768
                     /gene="aroC"
                     /locus_tag="KPK87_003261"
     CDS             3689..4768
                     /gene="aroC"
                     /locus_tag="KPK87_003261"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG2539376.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4771..5967
                     /gene="pheA"
                     /locus_tag="KPK87_003262"
     CDS             4771..5967
                     /gene="pheA"
                     /locus_tag="KPK87_003262"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG2539377.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVHFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6014..6823
                     /gene="aroE"
                     /locus_tag="KPK87_003263"
     CDS             6014..6823
                     /gene="aroE"
                     /locus_tag="KPK87_003263"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG2539378.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6835..7368
                     /locus_tag="KPK87_003264"
     CDS             6835..7368
                     /locus_tag="KPK87_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG2539379.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7389..8228
                     /locus_tag="KPK87_003265"
     CDS             7389..8228
                     /locus_tag="KPK87_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG2539380.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3511 a          847 c         1535 g         2672 t
ORIGIN      
        1 ggaggataat actatgaaag gaatattaaa agaactaaca gcaaaacttt atagcttttt
       61 ttactttatg ttctggggct tttattttag cgctggctat ttcttttaca aaaatatatg
      121 tacttccttt ctggtgaggc cttattttag tgattttgag cataagtaaa ttttataaaa
      181 atgattattt tatatattta tataagattt agtgcttgat agattatttt agagaactca
      241 ttagtgagtt ctctttttta ttttaaatat aaaattataa tattgggagg tagtaaaaat
      301 gatagtagta ttaaaaatgg gagcagataa aaatgaagtt aaaaaattaa ttgaggctat
      361 tgggagagaa ggagtagaag ttaatcccat agatggaaca gaattaactg ttttaggttt
      421 agttggagat actagtaaaa tagatgccaa aagaatagaa gcaaataagg tagttgaaaa
      481 agttatgcat gtggtagagc cttttaaaaa ggccaataga aaatttcatc cagaaccttc
      541 aatcataaat gttaatggaa tggaaattgg ttcaaagaaa attgctatga tagctgggcc
      601 ttgttctgtt gaaacagaag accaaatagt atctatagct aaagatgtga aaaaatcagg
      661 tgcaggattt ttgagaggtg gagcattcaa accaagaaca tcaccatacg ctttccaagg
      721 attgaaatat gatggattgg atttattaaa aaaagcaaaa gaaaaaacag gacttcctat
      781 agttacagaa ataatgtcaa cacaagatat agatatattt gaagaaaatg tagatgtaat
      841 tcaagttgga gctagaaaca tgcaaaactt tgatttatta aaagaattag gtaaaacaaa
      901 taaaacaata cttttaaaaa gaggattatc tgctacaatt gaagagtggc taatgtcagc
      961 agagtatata atggctggag gaaatgaaaa tgtagttctt tgtgaaagag gtataagaac
     1021 ttttgaaaca tatactagaa atactttgga tttaagtgca attttagctg tgaagaaact
     1081 tagtcattta ccagttatag ttgacccaag tcatgcagca ggtaaatcat ggatggttga
     1141 ctctttatct aaggcagcaa ttgcagttgg agcagatggt ctaataatag aagttcataa
     1201 tgacccagca catgcacttt gtgatggaaa acaatcgatt aagccaaatg aatatgatga
     1261 attgataagt gaattaaaga ctatcgcatc agcagttggt agagaaatat aatctataaa
     1321 aggatggtta aacgtggaag aaaaactatt agtaaattta aaagaaaata gctacaatat
     1381 atttattaaa aaaaatatac taaagaatat aggagaagaa ataaaaaaaa tatattttgg
     1441 aaataagatt tttattgtaa ctgatgaaaa tatagcaaaa ttatacttgg ataaagtaaa
     1501 aaataattta agtgaatctg gatttgaaat taaatcttta atattaaaat ctggtgaaga
     1561 aacaaaatca tttaatactc ttcctaaaat atacgatagt ttacttgact ttaagttaac
     1621 tagaaaagat ttgattatta ctttaggagg aggggtaata ggtgatttag gtggatttgc
     1681 agcagcaact tttttaagag gtataagttt tattcaaatt ccaacatctt tattagcaca
     1741 agttgatagt tctgtaggtg gaaaagttgg agttgacctt gagagaggca aaaatttagt
     1801 gggaagcttt taccaaccaa aggctgtgtt tatcgatcca aatgtattat tatcacttcc
     1861 agaaaaattc tatagagatg gaatggcaga agttataaaa tatggatgta taaaagacaa
     1921 aacttttttc aataaacttt taagtttaaa aaatagagaa gaagtaatgc aacatataga
     1981 atacattata cataaatgtt gctttattaa aaaaactgta gttgaagctg atgaaaaaga
     2041 ctttggagat agaatgcttc taaactttgg ccatacatta ggtcatgcta ttgagaaata
     2101 ctacaatttt acgggttata cccatggaga agctgttgcc ataggaatgt ataagataac
     2161 tttagaaagt gaaaagatag gaattacaaa aaaaggtaca tcagaactta taaaatcaat
     2221 tttaatgaat tataatttgc cttatgagat tgaattaaga gataataaat ctatagtaga
     2281 tgctatatct cttgataaaa aaaatctagg tgatacatta aaaataatac ttttaaaaga
     2341 tattggagag agtattatat ataatactac ttctaaattt tttcaggagg taagataatg
     2401 ggaagtctaa aaatatatcc aagtaaatta tctggagatg taaaaatacc accatcaaaa
     2461 agtatggcac atagggcagt tatttgttca tctctaagca atggaaaaag tagaatttct
     2521 aatatagatt tttctgatga tataatagct actataagag caatgacatc attaggagct
     2581 attatagaaa agaaagaaga tattttagaa atttcgggaa ttttttctaa agaaggtatt
     2641 ttaaatagag agaatcagtt gaatcaacct aaattgacaa tagattgtaa tgaatcaggc
     2701 tctacattga gatttttagt acctatatct ttaacatttg atggtgtaaa aaggtttata
     2761 ggtagaggta atcttggaaa aagaccactt gatacctatt atgagatatt tgacagacag
     2821 aatataaaat atagttacaa agaaaatcag ttagatttga ttatatcggg taagcttaaa
     2881 ccagatgaat ttagagtaaa aggaaatata agttctcagt ttatcacagg tttactgttt
     2941 atattgccta cgctagaaag tgattcaaag ataataatta ctactgagct agaatctaaa
     3001 ggatatttag atttaactct ttcaacgata aaagattttg gggtagaaat tataaacaat
     3061 aactataaag aatttattat aaaaggaaat caaacgtata aagcgagaga ttataaagta
     3121 gaaggagatt attctcaagg agcattttat ttatcagcag atgctatagg agaagatatt
     3181 agcatattag accttaaaga agattctctt caaggggata gtgaagtagt tgaaatttta
     3241 tctaggatgg gaatggaaat attaagagaa ggcaacaaaa taaaaggaat aacaaatggt
     3301 ttaaatggta ctttgataga tgcatctcaa tgcccagata taatacctgt attatctgta
     3361 gtagcatcct taagtatagg gaagactaca ataattaatg ctggtaggct tagaattaaa
     3421 gaatgtgata gacttcatgc aataaatgtt gagttaagta aacttggagc aaatatagag
     3481 gagaaagagg atagtttgat aatcgaagga gtaagcaagt taaatggtgg agtagaggtg
     3541 tggagtcaca aagaccatag aattgctatg acacttgcaa ttgcttcttg tagatgtgat
     3601 aaaccaataa tattaaaaga ttttgagtgt gtatcaaaat cttatccaca tttttttaaa
     3661 gattttaaga tgttaggagg tcgaattgat gagtggaata tggggaaata atttaaaagt
     3721 atcaattttt ggagaatctc atggaaatgc tataggtata aatatagatg gtcttccttc
     3781 ggggattgaa cttgaccttg ataagattga taaagaaatg aagagaagag cacctggaaa
     3841 aaattctata tctacttcaa gaaatgaaag tgatatacca gaaattctaa gtggatattt
     3901 taatggaaga actacaggaa cgccattatg tgcaatcata agaaatagtg atactcgttc
     3961 aaaagattat ggagagctta aaaatttaat gagaccagga catgcagatt tcacaggtaa
     4021 tgtgagatat tctggattta atgactatag aggtggaggt catttttcag gtaggataac
     4081 agctccatta gtattttgtg gggctatatg taaacaaatt ttatcacaaa aaggaataga
     4141 gataggagca cacataaaaa aaattaaaaa tatagaagat atgagttttg attatgtaaa
     4201 tatttcaaaa caacagttat caaatttgca aactttggaa ctacctttac ttgatttatc
     4261 aaaggaagaa gccatgaaaa atacgattat agatgcaaaa aatcaaggag attcagttgg
     4321 tggtataatt gagtgtgcag tagtaggtat taatgtaggg cttggaaatc cattttttga
     4381 ctctgttgaa tctactttat cacatttgtt gttttctgtt cctgctgtaa aaggtgtaga
     4441 gtttggttta ggttttgaac ttgcagatat gtatgggtct caaagtaatg atgaaatgta
     4501 ttatgaagga aatcaagtta agagcaaaac aaataacaat ggaggaatta taggagggat
     4561 tactactgga atgccaataa ttttcaaagt ggcaattaag ccaactccat caattagcag
     4621 acaacaaaac acagttaata taaaagataa aaaggacgat attttatata ttaaaggcag
     4681 acatgaccct tgtatagttc aaagggctat acctgtaata gaagcagtaa cagccattgg
     4741 aatttttgat ttgatgaaag gacgataata atggaggatt taacgagata tagaaatgag
     4801 attgacaata tagataaaga gcttattcaa ttatttgaaa agagaatgaa tacagttctt
     4861 gaaattgcca gacataaaat gaaaaataac actactatac ttcaaaaaga tagagaagaa
     4921 aaagtactaa gtagagccgt agataattta gataataaag catattcaca agaaacggta
     4981 cattttttta attctataat ggaaattagt agaggattac aaaaaagatt gatagataat
     5041 gatggtgagc agaaaatcga gtttagttta gattcatcaa ataagaattt tgatttgtca
     5101 aatgtaaata aatataagtc tttaaaagat gaattaaata aaaaaaatat cctagtagga
     5161 tttccaggaa aatcaggttc atttacagaa gaagcactaa ataaattctt caacaaaaaa
     5221 atatcaaaaa aacaatttaa agaatttgaa gatgttttca tagcacttaa aaataaagaa
     5281 ataaactatg gaataatccc catagaaaat tcatctacag gagctatctc tgaaacttat
     5341 gaccttttga gaaagtacgg tttttatata gttggagaag aatgtataaa aatagaccaa
     5401 aatttaatag ggataaaaga cacaaagctt gaagatataa aagaaatata ttctcatcca
     5461 caaggtatag gacaatgtag tgaattttta aaacaaaata gtacatggaa attgatacca
     5521 tttaataaca cagcaacaag tgcagaactt gtaagaaaac ttcaagataa aacaaaagca
     5581 gcaattgcaa gcaaaaaagc tgcaagtatt tatggattag aaataataag tccttgtata
     5641 aatgatatag caaacaacta tacaagattt gtagtaatca gtaatcaaat acacattgaa
     5701 gaagaatcaa acaaaatgag tgtagtattt tctgttgaac atgaagcagg aaaactttat
     5761 aaagtacttg gatactttgc taaaaataat ataaatatga caaaaataga atcaagacct
     5821 atgaaaaatg cctcatggag atatttcttt tatatagatt ttgagtgttc tatatacaat
     5881 gctaaagtgt acgatttatt agaattgata gaacataata cagcatattt taaatttatg
     5941 ggagtatata aaaataaaac tgaatagata agataaattt ttatttaatt tataattatt
     6001 aggaggtgta agtatgaatt ttttcggtct agtaggtgaa aaactctctc acagtgtttc
     6061 acctcaaatc cacaagagag tattcgagat attgaatatt gaaagtgctt ataaaaactt
     6121 tgaaatttca aaagaagata tatctaagct agacggtgct ataaaattat taggaataca
     6181 aggggtaaat gttactgtac catataagga gcgtattatg aaatatcttg attttatatc
     6241 accagaggca aaacgtatag gagcagttaa tacaatttta cttagagaaa atatgttata
     6301 tggttacaat acagattact ttggtcttga tagtatgttt aaaatggcta atattgatgt
     6361 tcaaggaaaa gtagcagtta tattaggaac tggaggtgct tctaaagctg ctcttacata
     6421 ttttattgat tctggtatag aaaagcttta tgtttcaact agaaaaaagg atgataaaaa
     6481 attattaaat agtaaagcta tattaataga ttatgaagaa cttaaacata taaaaggaga
     6541 tataatatta aatgctacac cagtaggtat gtaccctaat gtaggaattt caccagtgtc
     6601 aaaatctatt atacaaaatt ttgatatatt aatagattta atttacaatc ctggagaaac
     6661 agagttttta agaattggta atagtatggg taaaaaaact tgtgatggat tatatatgct
     6721 ggtggggcaa gcaataaaat ctcaggaaat ttggcaagat actaaaatag ataatagtat
     6781 tttagatgtt atatataatg aactaaaatt agaatttttg taagggagat tgttatgata
     6841 aataaaacaa aagaaaaatt gatattaata ggtatgccag gtagtggaaa aactacaatt
     6901 ggtaaacttt tggccaaaga atataattgt agtttctgtg atatggatga ttatataatt
     6961 caaatatctc aaaaaagtat agcagagttg ttttcagaag gagaagatat ctttaggaac
     7021 tatgagactc aagcatgtag agaactttct ataagtgata aaacagtgat atctacaggt
     7081 ggaggtgtaa taaaaaagga tgttaatatg gaaatactaa aagaaacagg tataataatt
     7141 tttattgata gacctataca aaagattttg gaagatataa atattaattc aagaccatta
     7201 ttaaaaaacg gaaaagatag attgtataat ctttacaatg agagaataaa tctttataaa
     7261 aaattttcag atatagagat tttaaatgat aaatcattaa ataatgctgt atataatatt
     7321 acaaatgcag tcagtgaaaa ttttaaattt gactttaaag aaaaataatc tagggggaag
     7381 taaaaataat gaatattgtt attgtaggac taggagttat aggaggctct tttgctaaag
     7441 cattaaaaaa agctggatat gaaaatgtat ttggagtaga tgtagattta gaaactttaa
     7501 aaaaagcaga aaaagcaaaa atcataaaaa aaggatgtac tacaggtaaa gagttattta
     7561 aaaaagctga tttaattata ctatctatat atcctagatt agttgtagat tttctaaata
     7621 ataataaaaa cttttttaaa aaagggacta taattactga tactacagga ataaaagaaa
     7681 cacttatcaa tgatgtgttg caaattattc cagatgatat agattttata tttggacatc
     7741 caatggcagg tagggaaaaa aagggtattg attttgcaag tgaacaagtt tttaatggcg
     7801 caaattatat aattactcca actggcagaa acaacataaa aaatttagag ttagttgaaa
     7861 atttaatttt agagattgga tttaaaagag taaaaaagtt aacttcacaa aaacatgatg
     7921 agattatagc ttttacttct cagttacctc atgttatggc agttgctctt ataaatagtg
     7981 atgaagaagg tagagatacg ggtaaattta ttggagatag ttatagagat ttaactagaa
     8041 ttgcaaatat gaatgaagac ttatggagtg aacttttttt aggaaataga gataatttac
     8101 taaaagttat agaaaacttt gaatcagaag taaatttagt aaaagaagct atatttaaca
     8161 atgataaaaa taaattaata gagtatttta aaaaatcatc aataagaaga gaagctttag
     8221 aaaaataaat attaatattt aagctatata tagatgggta taaatataag cgatagtgta
     8281 tttaattgta ttaataaata tggattcaat ataattaaat atgctattga tatatttgta
     8341 cccttaacta ttgttatctt ttaaatgata atgatattta tttaaatact ctttattaac
     8401 aatttataaa acttaattgt aaaaaatact tattaatagt aaaataaagt gaatcaatta
     8461 tattataatt gctaaagaga gtgaggtgta gctatttgaa aaaaaataga ttaatagcaa
     8521 agaatatatc aatagcattt atagtactat tcttttttag tgtat
//
LOCUS       DAEMIK010000160         8544 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEMIK010000160 DAEMIK010000000
VERSION     DAEMIK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.163"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            149..1369
                     /locus_tag="KPK87_003266"
     CDS             149..1369
                     /locus_tag="KPK87_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2539381.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1406..2755
                     /locus_tag="KPK87_003267"
     CDS             1406..2755
                     /locus_tag="KPK87_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2539382.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(2887..3180)
                     /locus_tag="KPK87_003268"
     CDS             complement(2887..3180)
                     /locus_tag="KPK87_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539383.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(3666..3869)
                     /locus_tag="KPK87_003269"
     CDS             complement(3666..3869)
                     /locus_tag="KPK87_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539384.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            complement(3920..4171)
                     /locus_tag="KPK87_003270"
     CDS             complement(3920..4171)
                     /locus_tag="KPK87_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBG2539385.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            complement(4187..5257)
                     /locus_tag="KPK87_003271"
     CDS             complement(4187..5257)
                     /locus_tag="KPK87_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG2539386.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            complement(5286..6707)
                     /gene="ccpM"
                     /locus_tag="KPK87_003272"
     CDS             complement(5286..6707)
                     /gene="ccpM"
                     /locus_tag="KPK87_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG2539387.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            complement(6729..8501)
                     /locus_tag="KPK87_003273"
     CDS             complement(6729..8501)
                     /locus_tag="KPK87_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539388.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
BASE COUNT         3081 a         1271 c          942 g         3250 t
ORIGIN      
        1 atgcaatcat tttttatact atacatatag ataaatgatt atatttattc aatattctta
       61 taaaaaacaa aattctccat cgaaaattat ggcataataa ttgcttatta tattaataaa
      121 acaataaaaa gcaattggag gaataaaaat gcaacaggct ttgaaattaa aatatcaaac
      181 caaagaatta ggaaaagatt ttgtaataga aagttgtaat tctataaaac catggcttat
      241 aaacataaga agagaattac ataaaattcc agaattagct cttgaagaaa atcttactaa
      301 acagaaggta atttcttatt taaaagaaat cggaattgat tatatggaat ttacaaaaca
      361 caatggaatt atggcatata ttttaaaaga aagtgcagat aaaacgattt gtataagagc
      421 agatatggat gctcttccta ttgaagaaga aaacaacata ccttataaat caatacattc
      481 tggaaaaatg catgcttgtg gccatgatgc acatacaacc atgctacttg gagcatgtaa
      541 agttttacat tcaataaaag ataaacttaa tgtaaatgta aaattcttat ttcaaccagc
      601 agaagaaggt tttggaggag caaaattttt agttgaggat gggtgtttag aaaaccccaa
      661 ggccgattat atattcggac ttcatgttat gccacatata gaaacaggcc ttatcgaaac
      721 aaaatatgat acattaaatg ctagtgtaga cactataaaa ataagtatca aaggtaaaag
      781 agctcatggt gcatatcctg aaaacggtat tgatgcaata gttacagctt cacaaattgt
      841 tacatcatta caaactataa taagtagaaa cttagaacca aataatgctg ctgttttgac
      901 tataggtaaa atatatggtg gtgatgcaca caatgtaata tgtgaagatg taaaattaga
      961 aggcacttta agaaccttaa atagcaaaac aagaaacttt atgattgata aaatagctaa
     1021 aattgttgga gatactgcat ctgcatttgg ttgtgttggg actttacatg taagtgatga
     1081 aaactatcct gctgttataa atgaaaaaga attagtagat acagttatat caaatactaa
     1141 agaattactt ggagaggaaa aatttatatt gagaccaaac ccgtctttag gtggtgaaga
     1201 cttttctttt tacacagaac attgtaaagg agcattcttc catttaggat gtaaaaatga
     1261 ggaaaagggc ttaatttcac ctcttcatac ctctagcttt aatatagatg aagattgttt
     1321 accaataggt gttatgatgc atgtaatgaa tactttgtat tttaattagt acagtaatta
     1381 ataaaagaat aggagataaa ttaatatgga aaaagctaag aaattaggtc tatttagtat
     1441 gatattatta gggattaata gcataatagg ttcaggtata ttcttactac caggtaaagt
     1501 ttataatcta gcaggtcaaa acagtatgtt tatatacatt tttgctacac tcttagttct
     1561 atcaatactt ttatgttttg cagaagttgg aagcatgttt gataagaatg gaggagcata
     1621 tctatactcc aaaaaagctt ttggagattt tataggattt gaagttggta caatgtcttg
     1681 ggttattaga attatatcat ggtctactct agcagtagga tttgcaacag ctttaggttc
     1741 attttggcca gaatctgcca ctgaatacaa aggttatata gctgcaatac tcgtaacact
     1801 actttcaata aatagcttat ttggaattaa aagcacaaag ataatgaata atgtaataac
     1861 tatagctaaa ttagtaccat taatagtttt tataatagtt ggaatattct ttataaaatt
     1921 tgtaaatatt gttccttcag gaaatgtagt taactctagt atgggtccag ccattatatt
     1981 agtattttac gcatttaccg gctttgaatc atttattgtg gcaagtggag aaatggaaaa
     2041 tcctaaaaaa aatcttccag tcgcattaat aaccacaata tttatatgtg caataattta
     2101 tatattaatt caaattgtct gtatgggtat acttggagat agactatttg aaaacagtat
     2161 tccaattgca gatgcttcaa gtgtatttgt aggcaattat ggtaaagttt tcatatctgt
     2221 agcaacatta atatctatat ttggtataaa tataggttca tcaatagtaa ctcctaaatg
     2281 tggttcttct ctagcagaag aaggttctct tcctgcattt ataggaaaaa caaataaata
     2341 tggtgctcca tatgtagcaa taataatatc tttaatttgt tgtataccac ttgtgctaac
     2401 tggaagtttt gaacaacttg cagtaatgag cgtaattgca agatttgctc agtacatacc
     2461 aacttgttta tcagtaatag ttttaagaaa aagaacagat gttaaggcct cttttaagat
     2521 tccatttggc cctgttatac caatagtagc tattttaggt agcttatggt tgttacaaca
     2581 agcttgggca gaagatattt caaaacctat aactcaaaat agagttttaa taggattagg
     2641 ggcaatgttg ttaatagctc ctctatatat atttatgaaa aaaaataagg aaatcgagca
     2701 taaaaataaa tcagaagatt ctaaattata tatttcgaga gaaaaaattc aataatattt
     2761 tatactatta cttctcataa aatataataa atataaaaaa attgtagata aaactatttt
     2821 ataaaattta tctacaattt ttttatataa acatatatca aaatcaaaat attattaatt
     2881 tatactttaa agattcccat ctttatcata ttttattctt ataagaaaat ataatcctat
     2941 tactactaac tctatcacta aaaatactcc ataggtaaga attgaatcaa tactatctaa
     3001 cccatttcta aaaaaaatta atgctatacc tgctgctata atccctatag cattttttct
     3061 tactgcattt tgcccaactc cataaaaacc tggcattttt ccttttattt cgacagcagt
     3121 taaaattatc ccaaacacta taaataatac tccaataagc atcataatca tactaatcat
     3181 ctaattacct ccttcactta aggaaatttc aatttctaaa atctaaaata aataatattt
     3241 attttttata ataatattgt atatccaatt aactattttg gaaattagta ccaataaata
     3301 atgtttaaat attgaaataa tatattaatt ctaatcaata ttattaatat atatatacat
     3361 ttttacaaat atatttaaaa actttacttt taattcttac tatttaaata agtataattt
     3421 attataataa cttttaatta atttatactt ttaaattgta aaaatcaccc ctaatcacta
     3481 tatttaccac tataataatt gaagaggtga tttatataac agatactatt tatgcagtta
     3541 ttataaaatc acactcttaa ttataatcaa ctgcatattt ttataataaa aggaaataaa
     3601 gtaagaaaga taaatttaat caaatatgct aaatcaattt aatagtatac ttattactga
     3661 aatatttaca ttccatcagg agttctttgg ccatgtatca atttattata atatgtatta
     3721 tatgatcctc ctcttgcatt ataatcatta ttcttgcagt tacaagatga agatgccatc
     3781 atcttacaag tataaggaca cataatagta caaacacctc ttgtctcaac acctatatta
     3841 ttcatttcta ttctttttcc aaaactcatt catatcctcc ccttttctat aagatcactt
     3901 actttatacc tttattaacc tataccatat tgattatttt tagaacatta tcaaaactcg
     3961 taaattgcct attcaaaagt ttttcctctg gaatttttat attaaagttt tctattacct
     4021 cgcaaaatac taaaagtaat tctcttggct gtatatgtaa agacggtgaa aaaaataatt
     4081 ctttttttaa attgttattt ttattaaaat taattccagt cacatcaaaa aatatattct
     4141 ctagtagtaa ttccttttca gtgtaattca taattaacct ccaattctaa atagatatta
     4201 ctttagcata ctcttttaat tgattcacta tattgtccac taattctagt aattgtttat
     4261 tatcatgtat aaaaaatgtt ttttcataat cttctatatc ttcaacaaaa aagttttcat
     4321 ctaatgacat atatgaaaat ttttgcttca cctctgtctc atatgtatct ataacttttt
     4381 tctctatact aaaatagtct atatttatac catgtcttcc tttaataaaa tatttaagtt
     4441 cttcacaata ttctctatca tatatacttg atggaataca taatatacca caatcattta
     4501 ttactgcatt agaaatttca aaagctctaa ttccaaaatt accaaccatt tttctagaga
     4561 aagggaataa ttctcctgga acttctataa ttattaaatc tggctttgcc tctacctctt
     4621 tatcttttat aaaatggtta aaattaataa tctttgtctt actacttatc gaatcatcaa
     4681 acataaactc tggtatagga atcattttaa ataaatagct ctctttttta gggccaaatt
     4741 gtaatattga aaaccccctt tttcttagtt cttctcctat ccttatattt acatcaaaag
     4801 tatctaaccc tccataaaga ccacaaataa atattattgg tgtgtttatc tgtgatatac
     4861 cctctatatc tcttatttca tatttttcta aattccattc attaatcttt ttgaattcat
     4921 aatgagatat atacctagat attttattaa gatcatcacg tgtatataat atctttttac
     4981 cttcttttag agctactttt aatttaggaa ttataaattt ttctaagtct agattttctt
     5041 ttgaatcaac aaaccatacc aaagaattct ttttaagttc actttgaaaa tcatttgtaa
     5101 ctttagtaat aatattatta acattatact ccttattgca taacccccag ccagaaggac
     5161 taaccagtga attaatttct atatctgaaa ttttattgca atgatttaaa atagtaaaaa
     5221 aattataatt aaatggatat ataagtactt tttccataat cccatctttc ctttcattag
     5281 aaaatttaat attgatatcc tagagatttc aaaacgcagt aatctttcat attattttct
     5341 gtagaatgtc taattctgtc acaatcatgt ggttttattt cattttcatt acatttagta
     5401 tcatcaatct tagctataca tatatcacaa taactatagg cccaacaatc agaacattgc
     5461 tcttcatata attttcctat atttaaaagt ttttcagcct tttctatatc tactccacta
     5521 tctaaatttc caatcatacc cattttagaa ctttcactta ctctctcaca tggaaaaaat
     5581 tctccatctg tagttacaaa tattctaaaa gctcctggta tacatggtcc accatgatgc
     5641 cacacatctg gtaactctaa tctaccttca atatttttct catatctaaa atcatctaat
     5701 aaggctcttt ctattcttga taaaggtgac atactctcat cttttataaa ttttaatttg
     5761 ttgagtaaag tcaagaatct atcataactc ttacctaata tatattcatc tgatgaaact
     5821 atttctttat cagaatagtt attattaaga gtggaagcta aaaagtttga atcttttaaa
     5881 gtctcatttt tagatataaa atcatctata gaaatgtaat cattttctga atttatcact
     5941 gtattaaatg taatattctt tttataatat tctggatatt tattttttat gtaacgtaca
     6001 ttttttaata caacatcatg agatccttct cctgtatttg caaattttct acttttatca
     6061 tgaatttctt ttcctccatc taaactaatc atagttgtta tattattttt tacaaagaat
     6121 tctattattt cctcattaaa taaagttgca tttgtagtta gattaaagta tatttccttt
     6181 ccttctccaa ctacttttac atattctact atctgcttaa tcaaatcaaa ctcaagtaag
     6241 ggttctcctc cataaaagcc aacactaact tcatttaatt gactagaatt cttaatcaaa
     6301 tagtcaatac ttctttttgc tacatctata ttcattcttt tatttgtatg aactctgttg
     6361 ctgtatgaac cagaataaat gcaatagtca caacgtaaat tacaattttg ggttacttgt
     6421 aaaataatag aggatagatt attttctata tgatatttta tatacttagt ttcagggtgc
     6481 attgttttac ttaccctttt attttttaga aatccataat taatcaaggt attaatttcg
     6541 tcttctattt ctatgttcca gtcactttct ttttctaaat tatgctcaat tttatttaga
     6601 taattataaa cagcagcatt tacctttaaa atcttatccg tattaacatc ataaataaaa
     6661 tatgattttc cagctttaaa tgtgtgtata aatggttttt gattcattaa ttaacctccc
     6721 tttaatattt atttgcagtt acaaaataaa ttaaaataaa cacccttttt gtccataagt
     6781 tcatcatgtc ttccactttc taacaacttt ccattttcaa gaactacaat attgttagtt
     6841 atcttttttg catgttggat atggtgcgtt ataaatattc caatcttatt ttcaaatgtt
     6901 tttttatata aagaaaggag ttcatttccc gcttcagaat ctaaagatga atctggttca
     6961 tcaaaaatgt acatatcagc atctttcata agagttctag atattgctac tttttgccat
     7021 tcacccatag aaagttgctt cttaccaaac caggttccta aaatagtttc taatccatct
     7081 tcctcataaa tacttggttt caatttagtt tctttaagtt tacttttaac tttttcttcc
     7141 aagttactct tatatacatc tcccatggcg acattttctt ttaatgtagc ctcatattta
     7201 atataatctt gaaacataca acttactttt ctaatgtatt catctttttc aatatccttt
     7261 aaattttttc catttatcaa gatgtcacct tcataatcat cataaaaacc taataatatt
     7321 tttaaaagtg tcgttttacc tgaaccattt tttcctagta tagcaatggg atcattttta
     7381 tttaaagtta aattaatatt ttttaaagaa tactctgatg aatgattgta tttatatgaa
     7441 atatttatta actctatttt atttatatcc cctatttttt caaatatttt tatatgttca
     7501 attgagtctt ctttacagtt ataatctaga aatttaaaca gtaaatctgt ataaaaaaga
     7561 tcttgtatta ctgaactaaa taacgaaaat attgattcaa cattatcctt tatagattga
     7621 actgcttgta tatatgtagt cgcatctcct attaaaatct tctttaaaat accttgaatt
     7681 attataaatc ctataattcc tgcagataca attgaatcta aaactgtaca taatagcgta
     7741 gcaataaaat tctttttagc tatattcata tcttgagata taatatcctc ttttaatttt
     7801 ttataatgat taataagttt agaaccaatt ctaaatacca taatctcttt gtaagcataa
     7861 ccttttgaca acaaataatc tatgtaccag gcctttcttt ctttccaagt tctgtttaca
     7921 ctaacatcat attgttcctt tcctaattta atggaatata atgatttcaa tacaggaata
     7981 acaataacaa ctattattag ccaaagttga aattgtgata atacaacaat aagagaaagt
     8041 gtagatactg attctcttat tattgaaata aggttttcag atagtatagt aatattaaat
     8101 cctttttgag attgagctct atttatcatg tcataagtat tatcatcctc aaaatctttt
     8161 aaactcaaag ttaaagattt ctctaacatc attgtttcaa tatgtttttc aaaattttta
     8221 ttaaacttta ttctataata tccataaaaa ctttgaaaaa ttgaaggaat tatatttatg
     8281 attaaataca taattaacaa aactaatatt tcttttacac ttttcgaaaa tgtttgcata
     8341 ttattaatga tattttgact tactaacaaa gaaatagatg gaacaatccc ctgaaaaatt
     8401 gccataaaca tagagataaa taaatatctt ttatcatgta gataaatcat attaaaacaa
     8461 tttcttacac ctttaaaaaa ttgaaattta ccattttcca caatacacct ccatacttaa
     8521 taaaattgaa ataaaatatt ttat
//
LOCUS       DAEMIK010000161         8365 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEMIK010000161 DAEMIK010000000
VERSION     DAEMIK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.164"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            219..1121
                     /locus_tag="KPK87_003274"
     CDS             219..1121
                     /locus_tag="KPK87_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539389.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPTVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            1108..1863
                     /locus_tag="KPK87_003275"
     CDS             1108..1863
                     /locus_tag="KPK87_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2539390.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1994..3016
                     /locus_tag="KPK87_003276"
     CDS             1994..3016
                     /locus_tag="KPK87_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2539391.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(3180..3737)
                     /locus_tag="KPK87_003277"
     CDS             complement(3180..3737)
                     /locus_tag="KPK87_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2539392.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(3743..4819)
                     /locus_tag="KPK87_003278"
     CDS             complement(3743..4819)
                     /locus_tag="KPK87_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG2539393.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(4820..5503)
                     /gene="modB"
                     /locus_tag="KPK87_003279"
     CDS             complement(4820..5503)
                     /gene="modB"
                     /locus_tag="KPK87_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG2539394.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(5652..6464)
                     /gene="modA"
                     /locus_tag="KPK87_003280"
     CDS             complement(5652..6464)
                     /gene="modA"
                     /locus_tag="KPK87_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2539395.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEEKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            6657..7586
                     /locus_tag="KPK87_003281"
     CDS             6657..7586
                     /locus_tag="KPK87_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539396.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFLSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(7747..8196)
                     /locus_tag="KPK87_003282"
     CDS             complement(7747..8196)
                     /locus_tag="KPK87_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539397.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFSKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
BASE COUNT         2870 a         1337 c         1087 g         3071 t
ORIGIN      
        1 atacagaccc cttaagacta ctaaaatttt gggattgtat ctatgttggc tatattgttt
       61 ataaagtttt catttaacta tataagagcc acatagatta aactatgtgg cttattcgtg
      121 tttttaccac atagttttgt acactatgtg gtttttttat acacaaaatt agcaaataat
      181 taaaattata aaaatatttt caaaggagtg ttttatttat gtcaggtatt atatctgtaa
      241 tgacgcaaag tttaatttta tcgattatgg cactgggggt ttatataact tacaaaatct
      301 tagattttcc agatatgtct gctgatggta gctacacaat gggtgcttct attgtagcat
      361 tttctcttac taatggtatt tctcctgtag tagcaacttt aatggctatc ttatgtggtt
      421 gtactgctgg tcttgttaca gggattcttc acattaagtt taagatatct aacttacttt
      481 ctggtatttt agttatggga atgctatact ctatcaattt aagaataatg ggaaaatcaa
      541 atattcctct atttagcttt aaacacttat tcaatgggga aatatctcct acagttttag
      601 ccttagcttt tgtattcatc tgtaaagtcc ttttagattt attcctaaaa acaggtcttg
      661 gatacacttt aaaaggtgtt ggtgataact ctcaaatgat taaatcccta ggtataaata
      721 taggttctat aaaaatacta ggacttatga tttcaaatgg acttatagct ttatctggta
      781 gtcttatggc tcagttcttg ggattctcag atgtaaatat gggtatagga actttagttc
      841 ttggaattgc ctctatcata attggtatta cactatttaa gaaatttact tttatcaaag
      901 atactactgc aataattgtt ggctctttta tataccaatt cactatatac tttgcaatga
      961 gtttaggaat gttatcaact gacttaaaac taataactgc catagttata atagcttttc
     1021 tagccactgg taacttaaat atttcactaa aaaaaaacaa atgcaaagct agtaccaaaa
     1081 ataaatcaga aaaaagaggt gttgtagatg ttacaaatta aaaatctttc aaagagtttc
     1141 cctaatccat atggtgaacc taatactatc tttgaaaatt tatcaatcga tatagaagat
     1201 ggtgaatttg taagtatcat aggtagtaat ggaactggta aatctacttt attaaatata
     1261 atatccgggc ttataaaaga atcttctggt caagtattat taaataaaca taacttgtct
     1321 aatttagctg aacacagaag aactcaaata gtaagtagag tttttcaaga cccaagtctt
     1381 ggaacttgcc cttctatgac tgttagagaa aacttgtctt tagctcttaa caaaggaaaa
     1441 ctattaaatc taagaaaatg tcttcgttac aaaagagatt tcttagaaaa tttattggaa
     1501 ggtgtatctc ttgatttaaa aaaatacctt gatgttcaag taaaattttt atcaggaggt
     1561 caaagacaat cattatcact aattatgtcg tgcttaacaa atccatcagt tttattactt
     1621 gatgaacata cagcagcact tgacccaaaa acatcaaatg aagtaattga attgactaat
     1681 aagattgtaa gggaaaaaaa tataactaca cttatggtta ctcacaactt aaaacacgct
     1741 cttcattatg gtgatagatt gataatgctt cataagggtg aagttgtatt agatgtaaaa
     1801 ggtaaagaaa aagaacaatt aacagttgaa gaaatcttag aaaaatttga atatgcagtt
     1861 taaaataata tatctattgt attaaactcg gctttaaaat aaagttaaaa ttacaataat
     1921 tatggttact ttgtaataat tcatatgaat atatgaacat atataataat aaatttttga
     1981 gggggatata aaaatgatta ataaaaaaag attggcaagt ttgattttag ctggtgctct
     2041 tagtatatct atgcttacag ggtgttcaca aggaggagac tctggtaatt caaaacaaga
     2101 aagtaattct aaaggtaaag aagttaaaaa aattggtata actcaactgg ttgaacatcc
     2161 agcattagat gccactagga caggttttgt aaaagcatta gaaaagaacg gatttaaaga
     2221 tggagaaaat attgatatag acttccaaaa tgctcaaaat gacatgccta ctacacaaag
     2281 tattgcgagt aaatttgcat ctgataagaa ggacttaata tttgctatat ctactccatc
     2341 agctcaggca gcatttaatg ctactaaaga tatacctata cttattactg ctgtgagtga
     2401 ccctgttgct gctggattag ttaaaactct tgaaaagcca ggcacaaatg tatctggaac
     2461 atcagatttt gtttctgtag acaaaggttt agaacttctt aaaatatttg ctcctaaggc
     2521 aaaaactata ggagttatgt acaatactag tgaagtaaat tccaaagttc aagttgatgc
     2581 tttaaaagaa tatgcatcta aaaatggatt taaagttgtt gaaaaaggta taactacttc
     2641 aaatgaagtt aaccaaggta tttctagttt agttggcaaa atagacgtat tatatgtccc
     2701 tacagataac ttagtggctt cttctatgcc tatagtctct aaaatagcta ctgagaataa
     2761 aatacctgtt atagcagctg aatctggtcc agttgaaaaa ggtgctcttg cttgtcaagg
     2821 tatcaactac gaaaaactag gttacaaaac aggtgaaatg gcagttaaaa ttttaaatgg
     2881 cgaatcagta tctgatatgc ctgtggctac atcagatgat acagatatca ttgttaatga
     2941 agatatatta aaagctttag gtatggaaaa accttctaat gaaaatattt cttatgtaaa
     3001 gactaaacaa gaatagaaag atattttcta tatacgatta atatgcctgt ttctataaat
     3061 taatttatag aaacaggcat atatttttat tttaatgaat tatctattta aagattatat
     3121 ttaaatgctc ttaatttgtt tgtataaatt ttacttgtat aaactttata caatcatatt
     3181 tattcttcta ttttctttat tactctacat ggatttccaa cagctacagt atttggtggt
     3241 atatcttttg tgactacact tcctgcacct attactacat tatcacctat agtaattcct
     3301 ggtgttatga ttactccccc acctatccat acattatcac ctatttttac aggagaacca
     3361 tactctatgc ctgaatttct aagttgtgca tcaattggat gataggctgt atagatttga
     3421 acgtttggag ctaacattac attgtcacct atttcaattt tacatacatc taaaaaaata
     3481 caatcataat ttgcaaagaa attttcgcct acatgtatgt tatatccata atcacaccta
     3541 atgttttgtt ctacatttat ttgcttgcca actgaaccaa atagttgtct taaaatatct
     3601 tctctttttt catattcatc ctctaaagta ttattaaaca atctagttaa ttttttacaa
     3661 tactctcttt cttttactaa caattcatca tttgcatagt atcctttccc agacaacatt
     3721 ttttcttttt cagtcatata gtttactcct ttttgttata attgtatcta aagcaaaata
     3781 aatattcctg tggaaaatct aaatatattc tgtcaccttt cttaaatctc attttacttt
     3841 tttccaattc tatttgaata ggtacacatt cgttagataa atcagttttt ctaacatata
     3901 tagtatatgt aaaaggattt tctacaatgt taatcaattc gaaacataac ttttcattta
     3961 aatcttcatt agaatccaag accttaatat gatgctccct gattcccaca tattctatat
     4021 tatcacctat ttctctattt aatgtcaact cgcatcccca gtctgtagca taaatagtat
     4081 ttttatccag tctatttaat ttggatatat ttttacaccc tgttatctta gcttctataa
     4141 ggtactttgg agattcaaag agctcttttt taggtctctt tggaagacct tctcctttgt
     4201 tgtacactat aatatcatca caaactcggt aagcttcttc aatatcatga gtaacaaata
     4261 gtatatctcc cttgtaatct tttaacatat tcataagttc tttttccata ttgcttctta
     4321 aatgatggtc cagtgctgag aaaggttcat ctaaaagtaa tatatctgga gatgttgcca
     4381 atgctctagc tagtgctacc ctttgttgtt gaccaccaga taattgccaa gggtatctcc
     4441 cctcaaaccc atttaattta agtatatcta agtaccttgc tactatttcc tttttttcac
     4501 tcttacttag ttttaaaagc ccaagttcta tattttgact tacagtcata tgtggaaaca
     4561 aagcatagtt ttgaaataaa aatcctacct ttctatcttg tgttgataaa tttatcttct
     4621 tttcagaatc aaatagaact ctatcattta atacaatttt acctcttgtt ggtttctcaa
     4681 gccctgctat acattttaat gtcatacttt ttccagaacc agattctccc aaaaacccca
     4741 gagtaccttt ttcttgttta atttctacct ttagtttaaa agaggataaa tctttttcaa
     4801 tatccacgta taagctcaac tatccacacc tctttcctat aattttctgc tgaaattccg
     4861 accaataatt tagtaataaa atcattgcta tagatattgc tacaataatc ataacccaca
     4921 tcatagcttt attcatatct ccgccttcaa cagcaaaaaa tatagctatt ggcatagttt
     4981 gagtttttcc aggtatattt ccagctatca taagtgtagc accaaattct ccaagagctc
     5041 tggcaaacga cagcaccaat ccaccaatta ttccaggcca tgctaatgga actgcaatct
     5101 taaaaaatac tttccattca ttaagaccta atgttctagc agctgacaac atattattat
     5161 caatctgttc aaaagctgac cttgaagttc tatacatcat aggaaaagac accactactg
     5221 ctgaaattac agttgctgtc caactaaata ttaaagtcct atcgaaattt aataaaaact
     5281 ttcctataaa tccattcttc ccacatagta ataataaaaa gaaacctaca actgttggtg
     5341 gcaaaattag tggaagtgta aatagtccat ctataagacc cttccactta cctctatagt
     5401 tagccataat atatgacaca aatattccta ttacaaaagt tattattgtt gacaaaatag
     5461 atgtcttaag tgatatccat aagggtgacc aatctgttcc catctttccc tccttaattt
     5521 taaatggaaa tagtgaaact aataactcta ttaatttcac tatttccatt ttattataat
     5581 acacaattat tttcaatttt catttattta tatataattt atacttaatt acacataatt
     5641 tatttctaag tttattctac ttttttatat ccaaattctt caaaaatctt ttgtcctgac
     5701 tcacttaata aaaattcttc aaatttctta gcagcatcta cgtttttact agctttaatt
     5761 actgaaactg gatatgtaat aggtgaatga gttttttcgt ctgttttttc aactactttt
     5821 atcttatcat tattgacagt atcactgaag taaacgaatc caacatcagc atttcctgat
     5881 tgcacccatg ctaaaacttc ttttacatct tttgcaaata ctagcttatc ttttaactta
     5941 tcttttaaat ttaaattagt taacacctca tctgcatact taccagctgg aacactttca
     6001 gcttcaccaa cagcaatttt cttcacttta tctgtagtca aatctttcat acctgataca
     6061 ctactgtcag cagaagaaat aagaactaaa tcatttttta ctaaatcttt catagtatca
     6121 gaaactaata acttctcttc atccaaagct ttcatttgtt tttgacctgc tgaaataaat
     6181 aaatcacaag gtgctccttg ttctatttgt tgttgtaatg agcctgatgc tccatagtta
     6241 actgtaagtt ttacatttga atcaactttt ttatattctt cttcaatctt tgccattgct
     6301 tcttttaaac ttgcagcagc cgatatattt aactctactg aatcactact tttagttgtt
     6361 ttttcttgtt ttccatcatc ttttttctct tcattactat tgcacccaac tgtccccaaa
     6421 gttaaacatg caactaatcc taatattccc aatatttttt tcataacaat ctcccttttt
     6481 gtaaatttga tacacatttt aacatgtttt attatgtttt atcatgtttt gttatatata
     6541 gtttaattat atatacatga taataaattt tcaagtaatt tatttacact acttttaaat
     6601 attttgctac attttccttt ttttaatgtt aaaataacct tatatggagg tggattatgg
     6661 actcattatc atctttgaca cctgtcgagg tagctgaatt attaaagata acaaaaaata
     6721 ctgtatatga attaatcaaa agaggtgaac ttccttctta taaagtaggg aaaaaaataa
     6781 gagttgatac taaagatgtt gaagaatata tcaacagcca aaaaactggt aaagtaaagg
     6841 atacacattt caacaataaa aatatagata taaataataa tttcaaaatt attatttctg
     6901 gtcaagatgt aatattagac attttagcta ggtctgttga aaaaaaattg gatggaataa
     6961 gtacatttct ctcttatata ggtagttata atgctttgta tgagttatat aatggcagag
     7021 tttctatagc atcatcccat ctatgggatt tcgaaacgga tgaatataat agcacttttg
     7081 ttaaaaagct attgccagga ataccatgtg tacttataaa tctagcttat agaatgcaag
     7141 gtttctatgt tgctaaagga aatcctaaag agataacaac ctgggaggat ttaacaaaac
     7201 caagtataac cattgtaaat agggaaaaag gttctggaac tagggtttta ctagatggta
     7261 aacttagact tctaaacttt aatggtaaac atataaaagg ttatgataat gaagaattat
     7321 cacatcttgg agttgcaagt actgtttctc gtggaatagc agatgttggt ttgggaaatg
     7381 aaaaagctgc tttacaagta aataatatcg actttattcc tcttcacaaa gagcgctatg
     7441 atttagttat taaaaaagaa gacttacaaa atccagttta ccaaactata attaacataa
     7501 taaactctcc tgagtttaaa gctgaacttc aaggtcttgg cggatatgat ttaactgata
     7561 ctggaaaaac tatagcaaaa gtttaagatt aaaatcgcaa tctattttat aagatttata
     7621 atttttcaca aaatttataa cttaaacacg aaataaattg cgattttagt gtctcaaaaa
     7681 atttatgtct ttcattttat catccaatac aaaatttaat ataattaatt gcgcaatcat
     7741 attatattaa tatatattta cataatagta tttttcttta atgtagatta acatatgata
     7801 tgttttaaac atcaataccc ttgctttctt atatatatgt ggcaacacat atgagtttaa
     7861 taaattatat acaatgttgc tttcttcttg tattttttca accaacaagt tatttataaa
     7921 attacctttc tcattttcaa taatctcact ccaatttatc ttaactttta atatatatct
     7981 tcccactagg tatacacact ctacaataaa taataaaaat acaatgcagt cagtacctgt
     8041 tgtcttatat aaattacccg atactaatgg ttttgagaag aaattataaa aattcaaagt
     8101 atcacccgta taatcaaaaa gtattaatag cactcctaga actctcaata ctttaatatt
     8161 caacatgttt ttatctttat tatttttgtt tttcatatat actacctctc ttttctttag
     8221 atttgattgt aatctaatac taactctttt atttgaggta taaatgaatt taattggtag
     8281 ttattttcat ttttactaga aattaattca cattactaaa caaaacttat atttaaacat
     8341 aaaaatagcc tatctatttt ttaat
//
LOCUS       DAEMIK010000162         8363 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEMIK010000162 DAEMIK010000000
VERSION     DAEMIK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.165"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(173..1804)
                     /gene="bclA2"
                     /locus_tag="KPK87_003283"
     CDS             complement(173..1804)
                     /gene="bclA2"
                     /locus_tag="KPK87_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG2539398.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGATGATGVAGA
                     IGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPTGPT
                     GANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTGPTGANGATGATGATG
                     ATGANGATGPTGATGATGVLATNNAQFTVSSSSLVNNTLVTFNSSFINGTNITFPTSS
                     TINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSVSLS
                     FLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2162..2701)
                     /gene="hpt"
                     /locus_tag="KPK87_003284"
     CDS             complement(2162..2701)
                     /gene="hpt"
                     /locus_tag="KPK87_003284"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG2539399.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3029..4336)
                     /locus_tag="KPK87_003285"
     CDS             complement(3029..4336)
                     /locus_tag="KPK87_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG2539400.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4761..6536)
                     /locus_tag="KPK87_003286"
     CDS             complement(4761..6536)
                     /locus_tag="KPK87_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2539401.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6748..7317)
                     /locus_tag="KPK87_003287"
     CDS             complement(6748..7317)
                     /locus_tag="KPK87_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2539402.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7501..8136)
                     /locus_tag="KPK87_003288"
     CDS             complement(7501..8136)
                     /locus_tag="KPK87_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2539403.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2401 a         1659 c          952 g         3351 t
ORIGIN      
        1 aatcttttca tcatataaac ctattaaata ttatcctaat ttattggtat atgaaatcat
       61 attgtttatt attttatagt cttttcatca tataaaccta ttaaatatta ttctatttat
      121 tggtatatga aatcatattg tttattattt tataatcttt tcatcatata aactattgta
      181 ttctataaac tgatacatat ccagctgcag aagtacctgt cgtgcctgaa ccaaatgtaa
      241 aacttaaagt aacagctgct gctctagcat caactaaaaa gcttaaactt acacttacat
      301 ctgatgaagt aagtgtattt actgcttttg caataaagtt attttgagct actccattaa
      361 agttagtagt aattgacata aatcctgcaa gtgaaagtgt ggcacgtata ccgaaagata
      421 cattgtatat ccctccaact gcaagattta tagtactact tgttggaaaa gttatattag
      481 ttccatttat aaatgatgaa ttaaatgtca ctaatgtatt attcactaaa cttgaagagg
      541 acactgtaaa ttgtgcattg tttgttgcta acactcctgt tgctcctgtc gccccggttg
      601 gacctgttgc tccatttgct ccggttgctc cggtcgcccc tgttgctcct gtcgctccat
      661 ttgccccggt tgggcctgtt ggtcctatcg ctcctgctac tccatttgct cccgttgctc
      721 ctactgctcc ggttgggcct gttgctccat ttgcccctgt tgctcctgtc gctcctgtcg
      781 ctcctgctac tccatttgct ccggttggac ctgttggacc tattggacct gctactccat
      841 tcgctcccgt tgctcctact gctccggttg ggcctgttgc tccatttgcc cctgttgctc
      901 ctgtcgctcc tgttggacct actgctccat ctgctcccgt tgggcctgtt gctcctactg
      961 ctcccgttgg acctattgcc ccagctactc ctgttgctcc tgtcgctcct gttggaccta
     1021 ccaaaccatt tgctccggtt gctccagttg ggcctgttgg gcctgttgga cctaccaaac
     1081 catctggacc tgttgctcct actgctcctg ttggacctac ctctccatct gctccggttg
     1141 gacctgttgc tcctattggg cctgttgccc cagttgttcc ggttgctcca gtcgctccta
     1201 tacttccggt tggacctgtt gctcctgtat ttcctgttgg tcctgtagca cctgttactc
     1261 catttgctcc ggttgctcct gtatttcctg ttggacctgt tattccattt gctccggttg
     1321 ctccggttgc tcctgttagt ccggttggac ctgttgctcc agttgctcct gtattccctg
     1381 ttggacctgt tattccattt gctccagtcg ctcctgtatt tcctgttggt cctgtagagc
     1441 ctgttattcc atttgctcca gttgctcctt tgtttcctgt tggacctgtt attccatttg
     1501 ctccggttgc tcctgtattc cctgtcggac ctgttattcc attcgctcca gtcgctcctg
     1561 tatttcctgt tggtcctgta gagcctgttg ttccatttgg tccagttgct cccatatttc
     1621 ctgttggacc cgttattcca ttcgctccag ttgctcccgt atttcctgtt ggtcctgtta
     1681 ttccatttgc tccggtcgct ccggttgcac ctctaggccc cgtcggtcct gttggaccag
     1741 tagcacctgt tggccctgtt ggacctacat cttgatataa acttggacct gaaatatcac
     1801 tcatataatt aatcctcctt ttttaaagtt aaagtattac tctataaata ttgagtatta
     1861 ctctatataa ttatatgaat cgacaatttc ttatgtgata atgtatctat ttgcttatat
     1921 caacatatat gctttgattc ttgaatttca ttactttttt gtaaaatctt ttacaattat
     1981 taatacaatt aaaaaactca tttcccatca attatttaaa atttttttat tatgttataa
     2041 aaaatagata attaaattgc ataaaattaa tttaatacta ctttaattac tttgtaaata
     2101 aaaaaacctc aacacgatta ataaaaatca tattaaggca tccacaaaat tatataattg
     2161 cttatctatc ttcatcagta acattgaata tataaggaac atttctataa taatcaccaa
     2221 aatcaaaccc atatccaacc acaaacttat cttctatttc aaatccacaa taatctggca
     2281 ctaaatcgac ttttcttctg cttggtttat ccaaaagaac acagcatttt aaactagcag
     2341 gatttttagc ttttagatgt tttaacacaa aatccatagt aagtgcagaa tcagtaatat
     2401 cgtctacaac caaaacatca tatcctgcta tatcagtagt cacatctgaa actactttaa
     2461 cccttccagt actttcttca ttgtttccat aactagatgt agtcataaaa ttaactctaa
     2521 gtggtaaatt tatatttcta actaagtctg cacaaaatat aaagctacct tttaatagag
     2581 aaactaccat aagattttta tctttataat ctttttctat aattgctcct aattccctta
     2641 atttatcttt aattttttct tctgaataca atacttccca tttttttgtt tctatatcca
     2701 taaaaagacc tccaattgta ataatccttc ttaattttat aagagattgc tcactttttc
     2761 aatactctta ttcaatatta gtcaatatta ttgagttttt atgttcattc aacaattatt
     2821 aacaatgtat cttatatttt aagtagttca tatatccaca ctaaattaca tactatatat
     2881 tttagacaaa aaaagaaaaa acttgcacta acaagttttt tctattatac ataaatttat
     2941 acataatcat attttattag aggatactaa ttaaatttat ttatgctatt tttgttatac
     3001 ctcataatac aaaacatatt ataaagtact agtcaacttt attcatatca ttcattacat
     3061 ttaagattat ctcatctgtt atagacatac ctttgtcact tactctacct aagttttgta
     3121 tactttgctc aacacttgaa ccaactatac catttagtgg tggtacaaaa caatcttgct
     3181 tagctattat agcactttgt atagctgcag atgctgcaga agataatttt aatgcacaac
     3241 cagctttcgc accgtcacat atcattccac ttaagtcagc tatcatattt tctatagctc
     3301 cttctatttg cttttcagta ccattcataa gccaagagat accagcagtt gctccagtag
     3361 aagcagcaac accacatcca catacagcag ataatcttcc tgtatagttc ttaatataac
     3421 cagttactag atgagatata gctaaagctt ttgctagtct ttcatcagat tgtgggaatt
     3481 ttttattata agcaacgatt ggaagaattg ctgttaatcc atgatttcca cttccatttg
     3541 aactcataac aggcattttt acccctgcca ttcttgcatc tgaagctcca gcagttaaca
     3601 tcattgcata atttataaca tcattaccta aaagaccttc ctctatagac tttttaattc
     3661 catacccgac accaatacca gttttttgtt ttaatccata ttcagccatc tcttcattca
     3721 tttttactcc atctagtaaa aattctatat ctttaaaatc aatttcttct acatttttta
     3781 ttaattcttg tatagttact gtatccatta aactttcagc tttttctgcc gcatcatttg
     3841 aagctgatac ttttggttca ttgtctaata atacttctcc atctttttct aagaacgtaa
     3901 aattatcatg tttagttctt attctcactt tagcaatatg attttttcct tttaacacaa
     3961 cttctataaa tactttctct cttgtatcag caggtgttat tgatagtgga gtattatcca
     4021 tataatcctc agctatttta acttcctctt ttgttagagt ttctaacaca cttaatccat
     4081 tttcagaatg tccacctact atacctagag ctgctgcaat ttttaatcct aatctttcag
     4141 tacctggtat acctacgcac ataccatttt tatagatact aggactaact aacatacgat
     4201 tttcaactat ttcttctcct aataattcct ttgcctttgc acatgctaga gctaaggcta
     4261 ctggttcagt acaccctact gctggtttaa cttctgcctt taacatttcc actaattctt
     4321 ttcttatatc tctcattata tataaacctc ccaattaagt ttagatttag tactcaaata
     4381 attaattaaa taatttctta tctatattta tagcaaaata catgccatat tttttatatc
     4441 tttttatatt tttagtctta tattctataa ccattggtat gactgcctct atttatgtat
     4501 tttttttatt tgtatagagt atttatattc tttaaaatgg agaaacatat gacaatttcg
     4561 caaaaagaca aaaaaaagtc tcaaaatgag attctatatc attttgagac ggctcaaatg
     4621 tattattttt taacttataa aaaccatttt ttaattcata aaaaaagatt ctgcaaaaca
     4681 cttatagtat aagtttaaag ctttctttaa agttatgaat ttagaaaaat ttaaatttta
     4741 tatagctttt tattatatta ctatgaaatt atattgtatt tttcaatttt tctatataat
     4801 gtagctcttg atattccaag tgctttagct gttaattctt tatctttttt ataatctttg
     4861 tacttattta aagcttttat tatctcaatc ttctctagtt catcaagtgg aattatccta
     4921 tcgcttttaa cttctaactc ttctacagag gtattcttaa ttttttgagg aattacattc
     4981 attgtgatta ttgaagaaga agacatattc acactatatt caataatatt ctgcaactct
     5041 cttatattcc ctggccactt atattccatc atagcatcaa caaccctgct atctatgtca
     5101 ataacatttt tatctaattt ttttgaatac tctttaacca tatgcttgat tataagtggg
     5161 atatcttctt ttctctctct taaacttggt aattttatag gtataacatt taatctataa
     5221 tataaatctt ctctaaaagt gccattttga accattcttt ctaaatcttt atttgtagca
     5281 gcaataattc ttacatctat tgaaacattt gatttacttc caattttatt aagttccttt
     5341 tcttgtaata ctctcaataa ctttgactgt aaatgcaaac tcatatctcc tatttcatct
     5401 aaaaatatac ttcccttatg agctacttca aacattccaa gctttccacc ttttttagct
     5461 ccagtaaaag ctccctcttc atatccaaat aattcactct ctaataaatt gtctggtata
     5521 gcagcacagt ttacagctat aaaaggatta tctactctat tgctatggtt gtgtatggct
     5581 cttgcaaata gctccttacc cgttccactt tccccagtaa taagtactgt tgatgttgat
     5641 ttagaagcta ttagggcttc ctttttagaa tttcttatag ctacactttc tccaattatg
     5701 ttttccaatt ttattctata atctttattc atcttattgt agttttttat agcatctctc
     5761 ttatctataa aatcaataac atatcccttt aactcatttt ttaatactat tttattgatg
     5821 ttgtatattc ccttcgaatc atatccgtat cttttataaa aaaaactaca ggatttgtcc
     5881 ttcttaaagt tatttataga aggtttcttt ataaaatcta attcgacaaa tatgtttttg
     5941 ccagttatac tatcttttat attgaaaagc tctttaaatt ttgaattata tttatcaatt
     6001 tttccatcca catcaataga cacaacagct ttgtccatat tgtttaatag tgtctctaat
     6061 tttttctttt ctaattcaag ctcataagtt ttaatttgtg cttttaattt attagatatt
     6121 aggtctgcca ttttgcctaa aaagtttata agaccacttt tattattttc tataatttgt
     6181 gcttgttctt ttgtaaaggc tataaggcct ataacaccat gagaatcgcc atcacatata
     6241 ataggacaac acacctctgc aaactctaca caagccccac ttttatagca ttccctacat
     6301 atatcactac tctttgggtc atcaattagt attgtaatct gttctgtaag tgatttttta
     6361 aaagccgtat atttatctac tttctcacct attttgtcta tatatactcc tgtacctgca
     6421 atcctaaata actccttatt tactatagtt acatctatat tgagtactga tactatagct
     6481 tcagctatat tttgaatatc agattttata tattctaaac ctatcataat catcattcct
     6541 tatttttctt tagcataagt atttgtctta tttctttaag aatcaagtat atcttattta
     6601 aagtaaattg gattgacatc aggattacaa agagcataac aattattatt aactcataat
     6661 ttatttccat ctaaacatct ccttataaca aaagactagg cttaacctag tcttttatat
     6721 tatatccata tttatatttt aactcattta tcttcttttt cccacataaa tatattttaa
     6781 tttatttgac tctttaatca taccatcaaa gtttaatctt gaaaatatat taaatacttc
     6841 atgtggatag tgaatctttg caacagaacc acaaaattta tgtttaagat agtttaactt
     6901 aacttttaaa ttttttaaat ttaaatcctc taatataacc tctccattgc cctttaacac
     6961 tctttgtatt tcagatgaag ctttttcaca atctgtaata acattaaatt ggtcaactat
     7021 tattatcttg ttgaactgat tatcatcaaa tggtagaaac tctaacttac ctttaactac
     7081 attgcaattt tcattataca ctagagaata acagacatct tcataattac tattctccag
     7141 tatagttaca gaagttccaa gacctctcaa tctcttgcct aggtttccta tatgaccaac
     7201 aagcaatatt ttatcaccta cattatactc aattgtatcc aaaaaacatt ctaattcatc
     7261 agatgtatat ttagctttaa ccggctgttt atttatctga cataatatgc ttttcacaat
     7321 gcttcacctc ttcatttcct caattataaa ttatgtacag attacattat aataaatggc
     7381 gagcaactat tcaagagata tttcattagt atttttttaa acaatcttta tacatttcaa
     7441 tatgatatta taccaaattt attaggattt tattcataaa aatcagtcta ttttttatgt
     7501 ttattcattg ttattttcat ccaatttttt tattttcgat atggatactt catatgccat
     7561 ttttttgacc acattatttt catctatttt tttctcatat tctctacttt gaaccctacc
     7621 ccaaagctgt attctatcac caacctttag attctttgca aatttagcat ttctacccca
     7681 aactattgat ggtatataat cagatttatt ataaggtcta tttatagcaa ctaataaatc
     7741 ggttatttcc cttcctaaag gtgtcttcct atacacaggt tccttacata catatccatc
     7801 taaaaatata ctattagggt ctttattttc ctcgtctatg ctttttattt ctctgacaaa
     7861 cactgtaagc actaatctat ttttattatt taatgttttg ttatatgatc ttaattggcc
     7921 tacgacattg actaaatttt gtttatcaag gtcaatatct tggagaagtc tttctgatac
     7981 agtcattgga agtatatcaa aagaatcgct tagtctgttt atttttattt tcatattata
     8041 aaatttttct ccaaatatct catggctaaa ttctagctta ttgtctaact cccctcttaa
     8101 gtttactacg ttattctcat ctttaacagc tatcatattt tatccctcca caattgttaa
     8161 ttcttatatt tctggtatca gcttatataa taaatatatt tataagggat gtattttatt
     8221 acaaatcttt gattgctgtt caaaagtttt tatttagtta taaatttcat ttaatatttc
     8281 acttttataa ttataaaatt attatctttt tgtaccacat atactaatta aactacctat
     8341 ttcagttttg tattaattat aaa
//
LOCUS       DAEMIK010000163         8139 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEMIK010000163 DAEMIK010000000
VERSION     DAEMIK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.166"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..598
                     /locus_tag="KPK87_003289"
     CDS             <1..598
                     /locus_tag="KPK87_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2539404.1"
                     /translation="MNNLTDKISKNNETKNEESIDSNLTKKENNIEENEDMSKASNIN
                     KSNKNINKEDDVKSKKNTVKNEDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKV
                     ADIGENKGNTSEATYQVAILSEKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMM
                     YYNNGDLKNSKLTLKKLVNKVPNSMYNNSKVKEILKEE"
     gene            714..2651
                     /gene="htpG"
                     /locus_tag="KPK87_003290"
     CDS             714..2651
                     /gene="htpG"
                     /locus_tag="KPK87_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG2539405.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYISDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEVVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            3039..4169
                     /locus_tag="KPK87_003291"
     CDS             3039..4169
                     /locus_tag="KPK87_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2539406.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            4396..5418
                     /gene="splB"
                     /locus_tag="KPK87_003292"
     CDS             4396..5418
                     /gene="splB"
                     /locus_tag="KPK87_003292"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG2539407.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            5600..6511
                     /locus_tag="KPK87_003293"
     CDS             5600..6511
                     /locus_tag="KPK87_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2539408.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            6526..6942
                     /locus_tag="KPK87_003294"
     CDS             6526..6942
                     /locus_tag="KPK87_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2539409.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(7042..7434)
                     /locus_tag="KPK87_003295"
     CDS             complement(7042..7434)
                     /locus_tag="KPK87_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539410.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            7575..7970
                     /locus_tag="KPK87_003296"
     CDS             7575..7970
                     /locus_tag="KPK87_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2539411.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSSKKIDVEL"
BASE COUNT         3290 a          832 c         1389 g         2628 t
ORIGIN      
        1 gatgaataat ttaactgata aaatatctaa aaataatgaa actaaaaatg aagagtcaat
       61 agattctaac ttgactaaga aagaaaataa tatagaagaa aatgaagata tgagtaaggc
      121 ttctaatata aataagagta ataaaaacat taataaagag gatgatgtaa aatctaaaaa
      181 aaatactgta aaaaatgaag atgataaaga ttctgactta tttacagagg atgaattgat
      241 gtctaaggcg aatactttaa aatcagaaaa gaaaactaaa tcttctatac aactatataa
      301 gaaagttgca gatataggtg aaaataaagg gaatacatct gaagcaactt atcaagttgc
      361 tattctcagt gaaaagttaa aagactataa aactgctgaa gaatattata aaatgtatgt
      421 agaaaattac tcagaaaaag atgcttattt tgatgaatct tattataatt taggtatgat
      481 gtactataac aatggtgatt taaaaaattc taaattgaca ttaaagaagt tggttaataa
      541 ggtaccaaat agtatgtata ataattcaaa agtaaaagaa attttaaaag aagaatagtt
      601 aaattagata agaaaaataa attattttat tttaaaataa tatgattata atatattgac
      661 ataccatttt aaaaaattta aaatattaat tataaaatga ggagggataa aaaatggaat
      721 ttgaaaaagg gagtatttca attcatacag aaaatatttt tcctataatt aaaaaatggt
      781 tatactctga taaagacata tttataagag agcttataag taatggatgt gatgcagtaa
      841 gtaaacataa aagacttgta tcactaggag aaatatcaga aaataagtca tcagattata
      901 aaattacagt atctgtaaat aaaggagaag gtacacttaa atttattgac aatggaattg
      961 gtatgactga agaagagata aaaaaataca taaatcaagt tgctttttca ggtgctgaag
     1021 atttctttaa taaatataag gataaaatgg aagagtcaaa tgatataata ggtcattttg
     1081 gattaggatt ttattctgca tttatggtgt ctaaaaaggt acaaatagat acactttcat
     1141 atacagaagg agcgactcct gtaagatgga tatctgaagg tggtacagaa tacgaaatat
     1201 ctgaatcaga tgctagaaat gatagaggaa ctactataac actatttatt gatgatgata
     1261 gtaaagaatt tttagatgaa tttactgtaa gaggaataat aaataaatac tgttctttct
     1321 taccagtaga aatatattta gaagatgttg aaagattaga aagagaagct aaagaagcag
     1381 aagaaaaagc taaaaaacaa aaggaagatg gaaaagaaga gattgtagat gcaaaagtaa
     1441 ttgagccttt aaacgacact aatcctctat ggttaaaatc tccaaaagac tgtactgatg
     1501 aggagtataa agagttttat agaaaggtat tcaatgtatt tgatgaacct ttattttgga
     1561 tacatttaaa tgtagattat cctttcaatt taaagggtat actttacttc cctaaattaa
     1621 aaaatgagtt tgaactgact gaaggaaaag taaaattata caacaatcaa gtgtttgtgg
     1681 ctgacaatat aaaagaagtt ataccagaat ttttactttt attaaaagga gttatagact
     1741 gcccagactt accattaaat gtatcaagaa gtttcttaca aaatgacagg gatgtaagca
     1801 aaatttctaa acatataatt aaaaaagtag ctgataagtt aaaatctctt tgtaaaaatg
     1861 aaagagaaga atacaataag ttttgggatg atatacaaat atttattaaa tatggttgct
     1921 taaaagatga aagtttctat gagaaggtaa aagaatgtat attattcaaa actattgatg
     1981 atgagtatat aacattacaa gattatttag aaaaatgtaa ggacaaacat gaaaataagg
     2041 tattttatat aagtgataaa gaacagcaat ctcaatacat taaactattt aaagaatacg
     2101 acttaagtgc tgttgtctta aactcatcaa tagatactca ttttatttca tttatggaat
     2161 ataaagagaa tggagttaag tttaatagaa tagatgcaga cctttctgat gtattaaaag
     2221 ataaaaatga aaataaagat agtgaagaaa acaaagaaga gatagctaag atagaaggtc
     2281 tgtttaaaga agttgttgga gaaagagtga agaattattc tgtagaagga ttgaaaaatg
     2341 aagacactcc agctatggtg ttagtatcag aacaatctat tagaatggca gaaatgcagt
     2401 caagatttgc tggaatggat ttaggaatga attttgaaga agaaaaaact cttgtaataa
     2461 atgagaacag tccaataatc aaaaaattgg tttctcttaa agatgatgag gaaaagaaag
     2521 ataagattac gcttatttgt aaccagatag cagatttagc tttactttct aataaagaat
     2581 taaagccaga tgagttagat tcatttgtac aaagaagtaa taagcttatg agtcttttga
     2641 tagaattata agattgaatt tgaatacatt gaagcaaatt tataaaaagt attcaatatc
     2701 agtgataaga aagaagtgtc tcaaaatgaa cttttcagtt catgaggcgc tttttttatg
     2761 tgaaattaaa tttattttaa tcatttcaac aatgaaaaat atatttatct ctattttgag
     2821 acagaccgtt ctttattttt ttatctttgg ttaattttta aatatttaag taggttctaa
     2881 taaaaaactc tatttagtta aatatagtta acttgatata taataagata atacatataa
     2941 ttaagtaaaa gattgttttt tagtttatgt ttaaatacat aattacactt tgttaaaaac
     3001 ataaaattat tactaaagta gaaataggag gttttagcat gaattttaat tataatttgc
     3061 cagtaaatat cttatttggg agaggcagaa ttaatgatgt gggaaaagaa gtatctaaat
     3121 atggcaaaaa agtattgata gtaacaggta aaaatagtac aaagaaaact ggattacttg
     3181 ataaaacaat agatttacta aaagactcta aaatagaata tgaagttttt gatagagtag
     3241 aacaaaatcc tcttataact actatatatt caggagtaga aattataaaa tcaactggat
     3301 gtgattgtgt tttagggctt ggtggtggaa gcattatgga tgctgccaag agtatagctt
     3361 tttcttataa aaacccagga gatttaaatg attatatatt tggaattagg caaggtgaag
     3421 aagctttacc tataatctta gttccaacaa catcaggtac aggtagtgaa ggaaattgtt
     3481 ttgcagtact tacaaattca gaaactaaag ataaaaaatc tataaagaag aatagtatgt
     3541 atgcaaaagc atcaataatt gacccagaac ttatggttac aatgccaaaa catataattg
     3601 catcagttgg atttgatgca cttgcccata atatggaagc atatctttct aatggaagaa
     3661 accctcttgc agatgtacaa gctatttatg ggattgaact tatatcagaa aacctaataa
     3721 aggtatataa tgatgttaat gatttagaag catgggaaaa aataacactt gcaagtacta
     3781 ttggaggaat gacgattggt acagcgggta cagccttgcc acatggaatg gagcatcctg
     3841 taagtggtct tagagatgta atgcatggaa aaggacttgc agctttgact cctactatta
     3901 tagaaagttc atgggatagt gatattgaaa gatttggtaa tatttcaaag gtattaggag
     3961 gaacttgtgc agaagattgt tcagatgcaa ttagaaattt ccttaaaaaa atagatttag
     4021 atataaaact aagtgattta ggtattgaaa aatcagatat agattggtta actgataatt
     4081 gtatgaaaat atcagttgca aatattaaga gacatccaaa gtattttaat aaggaacaaa
     4141 taaaggaaat gtaccataag tctttataat ttgactttac atatttagtt ttatataaaa
     4201 attataaatt aataataaaa aattataagt aattaatagt aaaaaaattg tgtatagaag
     4261 ttatatatat aataaatttg tcttaagctt ttattttact ttctaataaa taaaggagtt
     4321 taaaaaaggg aagattaatt aagtaatatt ttataaaatt cttcgattta aaaactttaa
     4381 gtagaggtga aataaatgtt taatcccaaa agaataatat tcgaaaaagg aactttagac
     4441 actgatgtgg gtaaaaaaat atacaataaa tttagagata ataaagatgt agagataatt
     4501 aatatatcat caaataggat taaagaacat attccaggtg aaaatctata tgaacaatat
     4561 agaagtggga agcagactat ggtagttggg attagaaaaa gtctgaagtt tcaaacatgt
     4621 aaaccttctg ctaattatca actaccactt gtcagtggat gtatgggaag atgtgagtat
     4681 tgttatttga acacacaatt aggtgataaa ccatttataa gggtgtttgt aaatgtagat
     4741 gaaatacttg aacagacaaa aaaatatatt gaagatagaa agccacaggt aaccatattt
     4801 gaaggggcag ctacatcaga cccagttcca gtagagccat atactaattc tttaaagaaa
     4861 acaatagagt tttttggaag agaggaaaaa ggaagattta gatttgttac taaatacagt
     4921 gatgtggata gcttattgga tgctgaacat aataatcata cagaaatacg atttagtatc
     4981 aatactccta gaattataaa tgaacatgag cattatacag cctctgcaaa taaaagaata
     5041 gagtcagctg taaaacttgc tagggctggt tatcagattg gtttcataat agcacctgtg
     5101 tttttatatg aaggttggaa agaagagtat aaaaatctga ttataagtat aaaagaagct
     5161 ttaccaaagg attttgataa aaaaattata tttgaagtta tatctcatag atatacaact
     5221 aaagcaaaaa atagaatact agagatttat ccagatacac tattgccaat ggaaaatgag
     5281 gatagaaagt ttaaatacgg tcaatttgga tatggtaagt acgtgtatga taaagaacaa
     5341 ttagatgata tgaaagagtt ctttcttaag gaaataaatg gagtatttaa aaatagttta
     5401 ataaaatata taatatagca aatcttacct aaagatttgc tatattttat ttttatacat
     5461 tttatgttta taataaagca atgagattta gcactatcat ataagaaaat taaaaagggc
     5521 actatctatc aagaacattt tttctagttt tgatactata aaggataaga gtaacaaaaa
     5581 tatcaggagg agttatagta tgcatgcatg ggagtctatt caaattacat tggatttaat
     5641 agaagcaaat ttatcagaag aaatatcaat taatgaatta gcaaataaag caaatttatc
     5701 accattttac tatcaaagac tatttaaaag attggtaaat aagactgtaa tggagtatat
     5761 aaagcttcgt cgtcttgcaa gagcttctga atacttagca gaacataaga atcgtatact
     5821 tgatgtagct ttaaattttg gatttgctag tcatgagaca tttacacgtt cctttaaaaa
     5881 agcatatgga ttaactccag aaaaatatag agcaaatcca gtaaaactca atcactttat
     5941 taagccagaa ttaattttaa attatgctat ggttgatgaa ggtgttccac tgattgcaga
     6001 tgatatagtg attgaagtca atcgtaagac attgagtaat ccaaggacat ttgttggaat
     6061 agaaatagaa gtacctattt gccagttggt gggcggtgaa acaactggaa ttgcaatagt
     6121 agaggaatta tggatgaaac ttggaagtca aagacataat ataccaaatc aaattccagg
     6181 tggaaatgaa tttgctgctc tttatatggg aaatgcaaaa gaaggaaatt gtatgtatat
     6241 ggcaggtgta gaggtagagt caggtacatc agtagaagga tatagtactt ttgagttgcc
     6301 agctaaagaa tatttagtat gtggatttga ggcagaaaac tttaatgagt tagttaattc
     6361 agctgttttt aaagcagata aatttatgga aagatggatg aagaaacata atttaacaac
     6421 tactgatttt gcaattgaga tgtattaccc cactacacct gaagcagcat atctggaaca
     6481 ttggattgtt ccggttccta ttgaacaata gaaaggatga tgaatatgaa ttggcaagat
     6541 gcgttttttc aagatactaa acctacattt gagcaaatta atgaatttat taataatcca
     6601 ttatgggaca agttaaataa aactttggtt tctacttata atgtagaacc taagcttgaa
     6661 tacagtaaat gttcaatgca aagaggttgg aatgtaaaat ataaaaaaag aggtaaatca
     6721 ctatgtactt tatatccaca agaaggttat tttaaagctt tggttattgt aagtgaaagc
     6781 aatagagtag aggttgattt atttattaat acttgttgtg attatatcaa aaaaatatat
     6841 aatgaggtaa acttttttaa tggaagtaaa tggctaatga ttcaagtaga tagtttattg
     6901 gtattaaacg atatgcttga actaattaaa tttagagctt aacatataga acaggatgaa
     6961 tatataagtc atcctgtttt tatattcaat cagataaatt aatatatagt ctataatatg
     7021 aataatttag taaatttaaa tttactttat ctttctacat tctttgcaac taagtccaca
     7081 tgtgtttttt tcttgcatat tagtagcacc ccaattagca aattgttgta aaataggcac
     7141 aatactttta cctttacttg ttaatgtata ttcaactttt ggaggaattt cattatattg
     7201 tttacgatga acaataccag tagactctaa atcttttaat gattgagcta gcatagtatt
     7261 tgtaatgcca tccaattttc tttttatgct actatatcta attggatttt caactgctaa
     7321 catacatata ataggtaatt tccacttacc accgacaatg tctaatgcat aacggattgg
     7381 acaggttaat tcttcaacgt ttttagttat atttttttca gtatttatac tcattttaat
     7441 tactccttag ttatagtagt atctttttta tactttatca gaaaaaactg catacttgtt
     7501 ttttctgtat tttatattaa aattatatca aaggataatc cttaatacaa gtatttaaaa
     7561 ggggtgtaag aattatgaaa gcattagaat ttttgaacaa ggcacgagtt tattatttag
     7621 caacagttga tgagaacaat caagcacatg tgcgtccttt aggatttgtt atggaataca
     7681 atggtaaact tacattttgc acatctaatc aaaaggatat gtttaagcaa cttattttta
     7741 atccaaaggt tgaactttgt tgtatagacc aaaaccttaa tactctacgt attttaggtg
     7801 aagcagtatt tgttacatca aaagaaacac aagctaaggc acttgaaata atgccaagtt
     7861 taggtcagat gtactctgta ggagatggta agtttgaaat attctctatt gataaggcaa
     7921 aagttagttg ttgttcaatg agtagtaaaa agattgatgt agagctttaa ttataattga
     7981 atcgacatcc atagtgaaaa ttaagaaaac gttattactg aaatgttgat agatttaatt
     8041 taaatgcaat aaaaagattt ttaaataatg aatatgtaaa aatttttagt tattagatgt
     8101 gtatttcaat aatgatatac tattttgaat aattacata
//
LOCUS       DAEMIK010000164         7960 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEMIK010000164 DAEMIK010000000
VERSION     DAEMIK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.167"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            122..1258
                     /locus_tag="KPK87_003297"
     CDS             122..1258
                     /locus_tag="KPK87_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539412.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            1251..2798
                     /locus_tag="KPK87_003298"
     CDS             1251..2798
                     /locus_tag="KPK87_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2539413.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            3132..3998
                     /locus_tag="KPK87_003299"
     CDS             3132..3998
                     /locus_tag="KPK87_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2539414.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            4139..5281
                     /locus_tag="KPK87_003300"
     CDS             4139..5281
                     /locus_tag="KPK87_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2539415.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            5665..6903
                     /locus_tag="KPK87_003301"
     CDS             5665..6903
                     /locus_tag="KPK87_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539416.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            6932..7819
                     /locus_tag="KPK87_003302"
     CDS             6932..7819
                     /locus_tag="KPK87_003302"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG2539417.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
BASE COUNT         3003 a          834 c         1334 g         2789 t
ORIGIN      
        1 cagacaaata gtgacctaaa aatcgtactt tgtttacata aataaaatag tatgatataa
       61 ttataaaaac aacttataat attgaagaag gttgcttgtt cgaaaaaggg gatgataaat
      121 tatgatagag ataagaaatg taacaaaaaa aataggaaat aatgttatat tagatgatat
      181 ttctctagtt gtagaaacag gtacattggt agttttgatt ggttcaagtg gttgtggaaa
      241 gactactact ttgaagctta taaacaaatt aataaaacct acatctggtg agatatatat
      301 taatggcaaa ccaatttctc aggaaaatga aatagagctt agaagaaaaa taggatatgt
      361 tatacagaac acaggcctat tccctcatct tacaataaaa gaaaatatag agctaattcc
      421 acgcctaaaa aaggaaaaat ccgttgaaga aattgaaaaa agaacgttgc aactacttga
      481 gatggtagga cttgatagtg atgaattttt gaataagtat ccttcagaac ttagtggtgg
      541 acagcaacaa agaatcggtg ttgcaagagc cattgctact gatgctgaaa taatattgat
      601 ggatgagcct tttagtgctt tagacccaat aacgagaaca tctttacaag aacaattatt
      661 ttctcttcaa gatgaattga aaaaaactat aatatttgta actcatgata tggatgaggc
      721 tttaaagata gctgataaaa tttgtattat gaaggatgga agaatagcac aatatgatac
      781 tcccgaaaat atacttagaa agcctgcaaa tgactttgta aaagatttta tcggagaaga
      841 tagagtttgg gataatccag aatatataaa agcaagagat attatgataa aaaatcctat
      901 tgctgttaat tctactagaa ctgtaactca aggaatagaa attatgagaa cgagtaaggt
      961 tgatagctta ctaattatag atagagcaaa aacattaaaa ggtattgtta ctgtaaaaga
     1021 tatgaaagat attgatgata agtcaatatt acttgctgat attatgtcaa gtgagccttt
     1081 gcatgtaaat gaaggtgata atttagtaga aatactaaat gtaatgaatc gtaattcggt
     1141 tggatatatt ccggttattt ctgatgaaaa taaactggta gggcttatta ctagaagtag
     1201 tttactttct gttttaagtg aacaattctt agaaatggag gtgagtgttc ttggctaatt
     1261 tttttaactt tatactatta caaaaagata agattataga gcttttaata caacatatga
     1321 gtctaactgt tacatctatt ttaatagcta taatagttgg tgttcctctt ggaataatta
     1381 tttcaagaat ctcatctctt agaaagtttg tacttggatt tgtaaactta gtccaagcag
     1441 ttccgtctat ggcacttttg ggtcttcttg tacctatact tggtatagga agtaaaccag
     1501 ccatatttat ggtagtggtt tattcattac taccaattgt aaaaaatacg tatataggta
     1561 taacaagtat tgacccagta gtattagaat cagctaaagg tattggtctt acaagaaatc
     1621 agacattatt taaaatacaa tttcctttag cgcttcctat aataatgggt ggagttagaa
     1681 tatctgcagt tacagctgtt ggtttgatga ctcttgcagc atttataggt gctggtggtt
     1741 tgggatattt agtattttca ggggttcaaa ctgtaaataa taatatgatt ttagcagggg
     1801 caatcccatc atgtataatt gcactgttag ttgattattt atttagtaaa attgaagttg
     1861 cagttactcc aaaaggatta aatccaaaag cacctaagaa gaattatata gcacttaaag
     1921 taatatctgt agtgcttgtt gtttcaatgt tgttcgttgt ttttagttct agtttttcaa
     1981 gtaaaaaaga tacaataact attggttcta aagattatac agaacaactt atacttggta
     2041 atgtttatgc tgaattagta gaaaaaaata cgaatcttaa agttaagaag aatttaaatc
     2101 taggtggttc atctgttgcg tttaatgcaa ttaaatctgg tgagttagac atgtatgttg
     2161 actatacagg tacacttttg gtaaatgtaa tgaaacatgc tccgattaaa gatgctgatg
     2221 aagcatataa tgttgtaaaa gatactatgg aaaaagaaaa tcagttaact ttacttgacc
     2281 ctcttggatt taataatact tacacattag ctatgatgcc tgaaactgct gaaaagtatg
     2341 gaataaatac tatttctgat ttgactaaat atggtaaaga atttacattc tcaccaactt
     2401 tggagtttga aaatagagaa gatggtcttg tagggctttc tagagattat ggtttaaaat
     2461 tcaaagatgt aaaagcaatg aatggaagtt taagatatac agctttagat aataatgaat
     2521 ctcaggttat agatgcattt ttaactgatg gtcttattaa aaagtttaat cttaagatat
     2581 tagaggacga taaaaatttc tttgtaccat attatgcagc accattagta agagaagata
     2641 ctttgaagaa atatcctgag ttagaaaaag ttatgaatat gctttcagga aaaataaatg
     2701 aagaaactat gagagaactt aactatcaag tggatgaact tggtaaatca ccagaagaag
     2761 ttgctcattc attcttagtt aaagaaggct tagtataaaa aattaatagt ttatatcaaa
     2821 aatttaaggg agtgtctcaa aatgaacttt ttagttcatg aggcactctt ttttatatga
     2881 aatcaaatat attttatcat ttcaacaata aaaaagaaga ttatctttat tttgagacag
     2941 tctcttattt ttatgtatac caaaaccata cctacgattt ttatttataa ttaagtttca
     3001 aatagaatga acaagtacaa ccaattttga atataaatat agtaaatgtg cttatatacg
     3061 attttattgt ctggttgatt gatattgata gatagctttc tttattgaat cgaagggagg
     3121 gggaatttgt tatggataac tataagaaag tgaagcaagt attgtggata attttatttg
     3181 ctaattttgc agtagcatta ttaaaaatca ttattggtaa tcaaataaaa agctatagta
     3241 tgacagcaga cggatttcat tctttgtcag atggagcatc taatatagtg gggcttatag
     3301 gtattttctt tgcatctaaa ccaaaagata aaaatcatcc ttatggacat aaaaagtttg
     3361 aaattataac cagtttattt ataagtggta tgttgtttgt tatagcaatt aagataattc
     3421 ttagtgcagt gttaagaata gctaatccag ttgtacctgc tataactata gaaagtttaa
     3481 ttgctttaat tattacactt tttataaata tttttgtatg catgtatgaa tatagaattg
     3541 ggactaaact aaatagttat gttttgattt cggattcatt gcatacaaga agtgatatat
     3601 ttgtgtctct aggtgtattg gttactttag ttggtgttaa gttaggattt ccagtaataa
     3661 tagaatctat agttcctatt attatttctg cattcataat ttattcggct tatggaatat
     3721 ttaggccatc tattggaatt ttggtcgata gagtggctgt agatgaggat tatataaaag
     3781 aaattgtgtt tgaatttaat gaggtaaggg atgttcacaa tatcagaagt agaggtagca
     3841 aaagttctat ttatattgac atgcatgtaa tggtagaccc atttataagc gtagagcaat
     3901 ctcatgattt aacacataaa attgagaaac aaatacaaga agaaattaat gaaaatgctc
     3961 aagtaatagt ccatattgaa cctttttata gtttttaact tagttataaa atacaagtta
     4021 taaaaaaata taatttataa aaaaaatatt ataccataaa taatttataa aaattattta
     4081 tggtataata ttttctataa agaaattaag tcttaatata tctaaggagg aatttacaat
     4141 gagatacgtt gaatatggaa agacagggaa aatggtttca gtagttggct atggaggatt
     4201 aaggtttgat ttagaaaaaa gtaatcaaga aaatgctgat ttagtcaaat atgcgtatga
     4261 aaaaggtatt aattactttg atacagcacc agggtattgt gatgacagaa gtgaagatat
     4321 atttggacta gcctttagac aaatgataaa ggaaggtaag accgattttt atgtatcaac
     4381 taaagaaaag ccaaaagcat gtgatacatc agaaaaagct atagaggctg taaaaaaatc
     4441 aatagaacga ttaggcgtat ctaaaataaa tttttatcat gtttggaata ttagaaagat
     4501 ggaacattac gaactagcaa tgagacctgg aggtcaatat gaaggattat tgaaatgtaa
     4561 agaagaagga ttgatagacc atatagtatt ttcatctcat caaccagggg atgaggttat
     4621 agatattcta agtgaaaata agtttgaagg tgtaactatg ggaataaata tattaaactt
     4681 tccatatagg ctaaaaggtg ccaaatatgc tgttgataat ggatatggtg ttgtggcaat
     4741 gaacccatta agtggtggaa ctattccaaa gtataataaa gaactttctt ttctatcaaa
     4801 agatggcgaa agtactatag aatcagcttt aagattcaac ataggaattt cacaaataag
     4861 tatatctttg atagggttta ataaaaaaca agatatagat gatgcatgta aaattgcaga
     4921 cgaaaataga atttattcag atgaggatat acatgatatt gagactagat taaataagaa
     4981 tatgaacgaa atatgtacag gttgtggtta ttgtaaagta tgtccaaaag gtattaatac
     5041 cccagcctat atgctttttt ataatgaaaa gcagatgttt aaaaagagtg atgaagaaat
     5101 gacaaagtta gtttatggct taggacattg gaattacaca atgaatagta aggcaaaagc
     5161 taaagaatgt atatcttgtg gaaaatgtga agttgaatgt actcagcatt tacctataat
     5221 agatagatta aaagaaatta aaaaatggga agaagatgga gctaatagtg ttaaagtcta
     5281 attaatttta tactatattt gaaatttgta tagtatttag taatgtttta aaatatagtt
     5341 ttaatattta aggagacaga ttataatttt gtctccttaa attatgcttt aatttgttat
     5401 agtttaattc cataagcaac attgtatatt tttatataaa gtttttcaaa tattttacat
     5461 taatattgct tacaaattct cattttatac aacactaatc tatgttatat aaacaatact
     5521 tgactgatta taacatcaat aaagtaaata ttgtggttgt aaataaagag ttattcaatt
     5581 acaataaaac aagtaagaac attatgaaga tggctattaa atataaggaa taaattgtag
     5641 gtgtcagcaa atcctatata atgaatggat ggtgatatat tgatgagtaa aatattaaaa
     5701 tataaaaacg agatgttttt gtttatatta gttgtaataa cttacttaat aataactaag
     5761 atattttttt ctaagactac tatattttat gatttaaaca atacttatga tgtattgtta
     5821 gatacagata caggagtttt attcaattta aatgtatttg ctataagtca agacaatagt
     5881 aagcacatat tgttttcagc aattatatca atttttgcat atccaatata cctattttgt
     5941 acttcaatag caaatcctgg gacaacagat tttaatagtg cctatgggtt tggactaatt
     6001 tgtctacaaa taataactag tgctatgtca ataactttag tctttaatca tattaaaaag
     6061 ataaaaatgc agagattaac tttaatttta cttactatga taatgatatt ttcttttcca
     6121 cagttattta tgactttaaa tgtagagaga tttatatata gccaattttc attaatattt
     6181 ttcatagtta ttgcaaataa gatgaaagga aaaaattctt atttagtaga attagcagct
     6241 attcctctat ttggaataac tattagtaat atatatttgt atttttttaa tatgatattt
     6301 gagtttaaat taaaaatagg aaaaatgtta aaacatttaa ttacatttat cctaatggca
     6361 tatatttgtg tagtttcaac aaagtcttat gagagtttta tgaaccttgg aaatgtaata
     6421 cagtatgata cgaaatttat aagtggagaa ccaattttgg aaaagattgc aatgattata
     6481 gagagattat tgtattcggt tttttatttt cctggagcaa gtattaaaaa agggttattt
     6541 ttgcaaaatg gagaagttgc tacaatacca gtaattctta cattattagc tttatgtttt
     6601 tgtgttttat cagttattga aaattcagaa aaaagagttc ctaagctatg tatgggaata
     6661 attatattca atctaacttt acatggtata gtaggatata atctagtcaa ttcaagtatt
     6721 atggctataa attttagttt tgcagttata attttattgg cttactttac gaaagcgctt
     6781 agaaaaaatg aaaagaatat gtataatata tttctatcgc tgttattggt tactatagta
     6841 atcagtaatg tcaatggatt tatagaaatt ttaaacatag ggataaaatc atatcctgtt
     6901 taaaaaataa tattgaaagg gtagtaagtt tatgaaacag accaatacaa gtataaggct
     6961 tcttactaaa atttcagatt atataaagtt aatgagaata aagcaatata taaagaattt
     7021 ctttgtattt tcagctataa ttttttcaaa caacatacta aatataaatt tatttttaaa
     7081 tacgtttatt gcgtttgtgt gtttttgtct aatgtcatca tcagtatatg cactgaatga
     7141 tgctatagac atggataaag ataaaaaaca tcctaaaaaa tgtaatagac cagtggcaag
     7201 tggaagaata agcaaaaaaa gtgctaatat actatttgta gtattagctt taatttcagt
     7261 ctgtttatca acagttgtta gttttaatct ttctataatt ctcagtctct atttgataaa
     7321 caatatatta tattctctta aactaaagaa tataatacta ttagatgtgt tttcaatatc
     7381 tcttggtttt atacttaggg tttatgcagg atgtgtagcc ataagtgtta gtttatcaaa
     7441 ttggataatt ttatgtacat ttttcttatc tctgtactta gctcttggaa aacgtaaaaa
     7501 agaaatagaa actctaagag atgatgcagt tgaacataga aaaatattgg aagattatga
     7561 catagaaaat ctaaatcaaa tgatgatagt tatattaagt tctacaattg tatgttatgc
     7621 attgtattct acaagtaatc cagaaaagcc acatatgata tttacaacaa tatttgtagt
     7681 ttatggtgta cttagatata attatataat taacactaca aatgaaaata atcctactga
     7741 tattgtctta aaagataatg ctttaaaaat aaacgtaatc ttatggataa ttacatgttt
     7801 aataatattg attttttagt gatattttta gtgataggat ggggaaatac ttatgaaaaa
     7861 aagtgtatta ttacttgatg tagattatac tgttataaat actgatagca tgatagattt
     7921 ttttatttac tctttaaaaa ataaaacctt taaaactatt
//
LOCUS       DAEMIK010000165         7798 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEMIK010000165 DAEMIK010000000
VERSION     DAEMIK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.168"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..984
                     /locus_tag="KPK87_003303"
     CDS             <1..984
                     /locus_tag="KPK87_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2539418.1"
                     /translation="TLLEMINASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKK
                     FLLSAGKTRSSILISCGARLAPFDIKELRDLMETDEMELDTIGDRKTALFVIISDTDD
                     TFNFVVSILYTQLFNLLCDKADDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRS
                     REISASIILQSQSQLKAIYKDNADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDS
                     FNTSENRGREVSHGLNYQKLGKQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKH
                     PNYKYLSDYDKKNTFDMEKHLRRRPALVKPDEVFDYYEISESDLQEDTDHE"
     gene            977..1144
                     /locus_tag="KPK87_003304"
     CDS             977..1144
                     /locus_tag="KPK87_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539419.1"
                     /translation="MNKRIRKKKDKKITEAINGLVFIAERREQQRQAAIRQFVKRCEA
                     LYEQKRVRREL"
     gene            1164..1331
                     /locus_tag="KPK87_003305"
     CDS             1164..1331
                     /locus_tag="KPK87_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539420.1"
                     /translation="MSRQKTKTDDIRREHYFTKMIENAENALREDLQERGGEQTLYVT
                     EYMQRRSYLLE"
     gene            1595..1810
                     /locus_tag="KPK87_003306"
     CDS             1595..1810
                     /locus_tag="KPK87_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG2539421.1"
                     /translation="MEFFNSAIDVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            1829..2698
                     /locus_tag="KPK87_003307"
     CDS             1829..2698
                     /locus_tag="KPK87_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539422.1"
                     /translation="MESILEAINEWIKEILIGAINGNLSTMFGDVNEKVGTIAAEVGK
                     TPQGWNANIFSMIQNLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     FFKAFVAVFLVTHTFDITMAVFDMAQHIVSGAAGVIGGDTNIDVTEALAAMQEGLKDM
                     EIPELLLLVMETSLVSLCMKIMSVLITVILYGRMIEIYAYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVGEMVLADNLHSAIFSLAAYTVILCFSLF
                     KSGALAKSIFNAH"
     gene            2740..3144
                     /locus_tag="KPK87_003308"
     CDS             2740..3144
                     /locus_tag="KPK87_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG2539423.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQILCFSVALLMGLPLFFLLK
                     DSAGTSLAAMAMIVVMLPCFLVAMYEKHGQPLEVVVKNIIQTKFTRPKVRPYQTENLY
                     ALLEKQRALEKEVSAIVKRTDKKDAGSRRKQA"
     gene            3092..5521
                     /locus_tag="KPK87_003309"
     CDS             3092..5521
                     /locus_tag="KPK87_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2539424.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAAVIRQAKGDGKAHTAQ
                     QTIPYLAMYPDGICKVAQHKYSKSIAFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     NVQLSFINQGSQQEQAARAIHIPPQNDDFNSIRTEYSDMLKSQLAKGNNGLVKHKYIT
                     FSIEADNPAAARARLSRIETDVLNNFKVLGVSAHPLSGYERLKVLHGVFHPAGEPFSF
                     SYDWLTPTGLTTKDFIAPSSFKFGEGRYFAMGKKTGAVSFLEIFAPELNDRILADMLD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GNEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCALTRLDY
                     QQEAGLMSSIPLGENLIPIQRGLTTSSTAIFIPFITQELFQTGAALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREITNAFLITDDDIFICDPEAEYFALVKRLG
                     GQVIRLSPTGKGMDGTPQYVNPMDMNLNYSEDDSPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRDYLADPVPEKMPILGDLYDELLKQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELNNRLVCFDIKQLGKQLKKLGMLIVQDQIWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNIKDLLASREVENIFENSDFVL
                     MLNQAQGDRAILAKQLNISPQQMKYVTHTEAGEGLIFYGNVVLPFVDHFPKDTELYRI
                     MTTKPEEVSSL"
     gene            5518..6477
                     /locus_tag="KPK87_003310"
     CDS             5518..6477
                     /locus_tag="KPK87_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBG2539425.1"
                     /translation="MKTDVIINRDALCALKELPSESVHCCVTSPPYFALRDYGLDAQI
                     GQEDTPEQYIDRLTSVFRELYRVLRKDGTLWLNIADTYCGTGNKGGYADPKNPKGRTG
                     QRIARNSRVTGCKQKDLIGIPWLLAFSLREQGWYLRSDIIWQKQNPMPESCKDRPTRC
                     YEHIFLLSKEKKYYYDAAAIAEPLAPTTAERYRRARSTNSKYTQEIPGQGKVQGLNRP
                     RDGGYYDDALMPTTRNKRDVWLINTVPYKGAHFAAFPPKLAETCILAGCPKGGIVIDP
                     FFGSGTTGFAAKSLDRHYIGIELNAEYCALARARIGGAGLSNN"
     gene            6504..>7798
                     /locus_tag="KPK87_003311"
     CDS             6504..>7798
                     /locus_tag="KPK87_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBG2539426.1"
                     /translation="MTRDGVVEVNETQQTAERISSREADSDFSQPDSAAAERVIEHLD
                     AAHTRKANKKVVKKEQEATALRTSTSRLQFTEEELAAPELERYIEKSNKAADRLDAAK
                     AAIPKQKKLVKERTFDEATGKAKTRLLFEKQEKPIPGGKKGNPLSRPAQEAGIFVHNK
                     IHSVEKDNSGVEGAHKSEELAERGAKYGARKLKQGYRSHKLKPYREAAKAEKAAFKAN
                     VNFQYHKTLHDNPQLTSNPLSRFMQKQQIKRQYAKAAKKGGAKAAAEATRKTAKKTAE
                     ETRKAAAFAARHPAGILIAVAALLLFIMISAGLSSCGAMFSGLTNGVLGTSYTSEDSD
                     LLAVENNYAALENGLQSEIDNIENTHLGYDEYRYDLDSIGHNPHELASYLTALLQSYT
                     PQSAQAELERIFAMQYTLTLTEEVEIRYRTETSTDPETG"
BASE COUNT         2231 a         1906 c         1963 g         1698 t
ORIGIN      
        1 acgctgcttg aaatgataaa tgccagtgaa gcccgcgagg acgacccgga atttcagtcc
       61 cccgttgacc tcatgtttga acggttggag gaaaaagacc cggaacactt tgccgtccgg
      121 cagtataaga aattcctgtt atctgcggga aagacaagaa gctctatcct catttcctgc
      181 ggtgcgcggc ttgcaccctt tgacattaaa gagctgcgcg accttatgga aaccgacgaa
      241 atggagcttg acaccatagg cgaccgcaag accgccctgt ttgtcattat cagcgatacc
      301 gacgatacct ttaactttgt agtatcaatc ctttacacgc agcttttcaa tctcctttgc
      361 gacaaggcag atgatgaata cggcgggcgg ctgcccgtcc atgtgcgctg tctgttagac
      421 gagtttgcaa atatcgggca gataccgaag tttgaaaagc tcatagccac tatccgaagc
      481 cgggaaatct ctgcttccat cattttgcag agccagtcgc agctaaaggc gatatacaaa
      541 gataacgctg atactatcgt cggcaactgc gacaccactc ttttcttggg cggcaaggag
      601 aaaaccaccc tcaaagaaat gtccgaaatc ttggggaaag aaaccattga cagcttcaac
      661 acttccgaga accgggggcg ggaggtatcg catgggctga actatcaaaa attaggtaag
      721 cagcttatga cggaagatga aattgcggtc atggacggcg ggaaatgtat tttacagcta
      781 cgaggggtgc gcccgttttt ctctgataag tacgacataa caaagcaccc caactataaa
      841 tacctgtctg actatgacaa gaaaaatacc tttgatatgg agaagcattt aaggcgcaga
      901 cccgcccttg tgaagccgga cgaagtattt gactactacg aaatcagcga aagcgatttg
      961 caggaggaca ccgaccatga ataaacgtat cagaaagaaa aaggacaaga aaatcacaga
     1021 agccataaac gggcttgttt tcattgccga gcgacgggaa cagcaacggc aggcagctat
     1081 ccggcagttt gtgaaacggt gtgaagccct gtatgagcag aaacgggtgc ggcgggaact
     1141 ttgattttac gggaggttac actatgagca gacagaaaac aaaaactgat gatatacgtc
     1201 gtgaacatta ttttacaaag atgatagaaa atgcagaaaa cgctctgcgg gaggatttgc
     1261 aggaacgcgg aggggaacag accctttacg ttaccgagta tatgcagcgc aggagctatc
     1321 ttttggaatg acgaaaaatg tgtgttcccg gctgaaaaga aaggaggaat tgacgcagta
     1381 acaaagaaac agacaactta cagaatacgc gcccctactg gaaaatccat aacgcccaca
     1441 ctcaaaactg aataccgccg cgcggcagac acataggcag cgcggggaac ccgccacaat
     1501 ccggcgggct tatgaccgcg gcagttacaa aactgaccgc cgttttttat gcccttttat
     1561 agggcaagcc gcatttgcgg aagaaaggag ctttatggaa ttttttaact ctgctatcga
     1621 cgtattacag acccttgtta tcgcactggg cgcaggctta ggcatttggg gcgttatcaa
     1681 tctcttggag ggatatggaa acgacaatcc gggtgcgaaa agtcaaggca ttaagcagct
     1741 catggcgggc ggcggcgttg cccttatcgg cactatcctt gtaccgcttc tttccggtct
     1801 gttcggataa agttacggag ggtagcctat ggagagcata ctggaagcga ttaacgaatg
     1861 gataaaagaa atccttatcg gagccattaa cggcaacctt tccactatgt tcggggacgt
     1921 aaacgaaaaa gtaggcacca ttgccgcgga ggtaggcaag accccgcagg ggtggaacgc
     1981 aaacattttc agcatgatac aaaacctgtc ggaaaatgtg attgtaccca ttgcggggct
     2041 tgtcattacc tatgtcctct gttatgagct tatcagcatg gtaacggaaa aaaacaatat
     2101 gcacgacata gaaacattca tgttctttaa gtggtttttc aaagcctttg ttgccgtttt
     2161 cttggtaacg catacctttg atataacaat ggcagtcttt gatatggcgc agcatattgt
     2221 gtccggtgcg gcgggggtaa tcggcggcga tacgaatatc gacgtaaccg aagcccttgc
     2281 cgccatgcag gagggactaa aagatatgga aatccccgaa ctactgttac ttgtcatgga
     2341 aacgagcctt gtaagccttt gtatgaaaat catgtccgta ctgataaccg ttatcctcta
     2401 cggcagaatg attgagatat acgcctactg ttcagtttcc cctatcccct ttgccaccat
     2461 gacaaaccgg gaatggggac agattgggaa caactaccta aaggggctgt ttgcactggg
     2521 ctttcaaggc ttcctcatta tgatatgcgt cggcatttac gcagttttgg taggcgaaat
     2581 ggtcttagcg gataacctgc acagcgcgat tttttccctt gcagcctata cggttatcct
     2641 ctgtttttcc ctgttcaaat ccggcgcact ggcaaaatct atttttaatg cacattaaca
     2701 cagcacacgc gggcaagccc cgcagaaagg aggtttttct tggcgtatgt acctgtacct
     2761 aaagacttat ccaaagtcaa aacaaaagtc gctttcaatc tgacaaagcg gcaaatcctt
     2821 tgtttttcag tggcacttct tatgggactg ccgcttttct ttttgctcaa agacagcgca
     2881 gggacgagcc ttgcggcaat ggcaatgatt gtcgttatgc ttccctgctt cttggttgcc
     2941 atgtatgaaa agcatggaca gccccttgaa gtggttgtaa agaacatcat tcagacaaaa
     3001 tttacccgtc ccaaagtgcg accgtatcag acggaaaacc tatatgcact cttggagaaa
     3061 cagcgggcat tagaaaagga ggtatcagcg attgtcaaaa ggacagacaa aaaagacgcg
     3121 ggaagccgca ggaaacaggc gtaagctgac ccgcgcggaa aagaaacaga tagcggcggt
     3181 tatccggcag gcaaaggggg acggcaaagc acacaccgca cagcagacca ttccctacct
     3241 tgccatgtac ccggacggta tctgcaaagt ggcacagcac aagtattcaa agagcattgc
     3301 ttttgaggac atcaactatc agcttgcaca ggcagacgat aagaccgcca tttttgaaaa
     3361 ctggtgcgat tttctcaact actttgacgc aagcgtgaac gtgcagcttt ctttcatcaa
     3421 tcaaggctca cagcaggaac aggcagcgag ggcaatccat atcccgcccc aaaatgacga
     3481 ttttaattct atccgcaccg agtattccga tatgctgaaa tcgcagcttg ccaaagggaa
     3541 caacgggctt gtaaagcaca agtacatcac gttttccatt gaagcggaca atccggcggc
     3601 agcaagggcg cgcctttccc gtattgaaac ggacgtactc aacaacttca aggtgcttgg
     3661 ggtatcggcg caccccttat ccggctatga acgcttaaag gtgctgcatg gggtattcca
     3721 cccggcaggc gagccgttct cattctctta tgactggctg accccgaccg ggcttactac
     3781 aaaggacttt atcgccccgt cctctttcaa gttcggggag ggacgctact ttgctatggg
     3841 gaaaaagacg ggggcggtat cgttccttga aatctttgcc ccggagctaa acgaccgtat
     3901 cttagcggat atgttagatt tggaaacagg cgtaatcgtc aacttacata tccgcagtat
     3961 cgaccagtcg gaagcaatca agaccattaa gcgcaaaatc actgaccttg acaagatgaa
     4021 gattgaggaa cagaaaaaag cagttagaag cggttatgac atggatataa tcccgtccga
     4081 ccttgccact tttggaaatg aagcaaagaa tttattgcag gatttacaga gccgcaacga
     4141 gcgaatgttt ctccttacgt tccttgtggt aaacatggca gacacgaaac ggaaactgga
     4201 aaatgacatt ttcgcggcgg cgggcattgc acagaaatac aactgcgcct tgacccgtct
     4261 tgattatcag caggaagcgg gactaatgtc ctctatccct ttgggcgaga accttatccc
     4321 tattcagaga gggttgacga cgagcagcac cgctattttt atcccgttca tcacgcagga
     4381 gctatttcag accggggctg ccctgtatta tggcttaaat gccctgtcaa acaacatgat
     4441 actctgcgac cgtaagcagc ttaaaaaccc gaatgggctt atcttgggaa caccgggcag
     4501 tggaaaatcc tttgcagcaa agcgggaaat cacaaatgcc tttctcatta ccgacgacga
     4561 tatttttatc tgcgacccgg aagcggaata ttttgccctt gtaaaacggc ttggcgggca
     4621 agtgatacgc ttatccccaa ccggaaaagg catggacggt acgccccagt atgtgaaccc
     4681 tatggatatg aaccttaatt acagcgagga cgacagcccc cttgcactga aaagcgattt
     4741 tatcctttcc ctctgcgagc ttgtcattgg cggcaaagag ggcttgcagc ccgtagataa
     4801 gacggttatt gaccgcgccg taaggaatgt gtaccgggac tatcttgctg accctgtacc
     4861 ggagaaaatg cctatcttgg gcgaccttta cgacgaactg ttgaagcagc cggagccgga
     4921 agctgcccgc attgcggcgg cattggagct gtatgtttcc ggtagcctta acgtattcaa
     4981 ccacagaacc aacgtggaac tgaataaccg ccttgtttgc tttgatatta agcagcttgg
     5041 aaaacagctt aaaaagttag gtatgctcat tgtgcaagac cagatatgga accgcgttac
     5101 cattaaccgg gcagaaaaga aatctacccg ctactatatg gacgaatttc atctgctctt
     5161 aaaagaggaa cagaccgccg cttatagtgt ggagatttgg aagcgttttc gtaaatgggg
     5221 cggcataccg acagctatca cacagaacat aaaggacttg cttgcttccc gcgaggtgga
     5281 aaatatcttt gaaaactctg attttgtcct catgcttaat caggcacagg gcgaccgggc
     5341 tatccttgca aagcagctta atatctcccc gcaacagatg aagtatgtaa cccacaccga
     5401 agcaggcgag ggacttatct tttatgggaa tgtggtgctg ccctttgtag accatttccc
     5461 gaaagacacc gagctttacc gcattatgac gacgaagccg gaggaagtga gcagcctatg
     5521 aaaaccgacg tgattatcaa cagggacgcg ctttgtgcct taaaagagct gccctctgaa
     5581 agcgtccatt gctgcgttac aagcccgccg tactttgcgc tgcgggacta tgggcttgac
     5641 gcacaaatcg gacaggagga tacgccggaa cagtatattg acaggctgac ctctgttttt
     5701 cgcgagcttt accgggtgct tagaaaggac ggtacgttat ggctgaatat cgcagacacc
     5761 tattgcggca caggaaataa aggcggctat gctgacccaa aaaacccaaa gggacgcacc
     5821 ggacagcgga ttgcaaggaa cagccgcgtt accggctgca aacaaaagga cttaatcggt
     5881 attccctggc tcttggcgtt ttccctgcgt gaacaggggt ggtatctacg gagcgatatt
     5941 atatggcaaa aacaaaatcc tatgccggaa agctgcaaag accgccctac ccgctgctat
     6001 gaacatatct ttctgctttc aaaggaaaag aaatattact atgacgctgc cgccattgcg
     6061 gaacccttag cacctactac cgcagaacgg tatcgcagag cgcgcagcac caacagcaaa
     6121 tacacacagg aaatacccgg acaaggaaag gtgcagggac taaaccgacc gcgggacggt
     6181 ggctactatg acgacgcgct catgccgacc acgcggaaca agcgggacgt gtggctgatt
     6241 aacactgtcc catataaagg ggcgcatttt gcagcttttc ccccaaaatt agcggaaacc
     6301 tgtatcttgg cgggctgtcc caaaggtggg attgtcatag acccgttttt cggaagcgga
     6361 accacaggat ttgcagcaaa aagccttgac cgccattata tcgggattga attaaatgca
     6421 gagtattgcg cccttgcgag ggcgcggatt ggaggtgcag gactatcaaa caattaaaac
     6481 cacgcgataa aatcacacag aaaatgaccc gcgacggcgt ggtggaagtc aacgaaacac
     6541 agcagaccgc cgagcgtatc agcagccggg aagcagacag cgacttttca cagccggaca
     6601 gtgccgcagc ggaacgcgtc atagaacatc ttgacgcagc acatacccga aaggcaaaca
     6661 aaaaggtagt caaaaaagag caggaagcta ctgctttgcg tacttccact tcccgcttgc
     6721 agtttaccga ggaagaactt gccgccccgg aactggaacg ctatattgag aaatcaaaca
     6781 aagccgctga ccgactggac gcggcaaagg cagctatccc aaagcaaaag aaactggtta
     6841 aggagcgcac ctttgatgaa gccacaggaa aagcaaagac ccgcttactc tttgaaaaac
     6901 aggaaaaacc gatacccggc ggcaaaaagg gaaacccgct gtcccgccct gcacaggaag
     6961 cggggatttt cgtccacaac aagatacatt cggttgaaaa agacaattcc ggcgttgagg
     7021 gggcgcataa gtcggaggaa cttgccgagc gcggcgcgaa atacggggcg cggaagttaa
     7081 aacagggcta ccgcagccat aagctgaaac cctaccggga agcagcgaag gcagaaaagg
     7141 cagcatttaa ggcaaacgtc aacttccagt atcacaagac cctgcacgac aacccgcagc
     7201 ttacctctaa cccgctttcc cgtttcatgc agaaacagca gataaaacgc cagtatgcaa
     7261 aggcggcaaa gaaaggaggg gcaaaagccg cagcggaagc cacaagaaag acggcgaaga
     7321 aaaccgccga ggaaacgagg aaagccgccg catttgcggc aagacacccg gcgggcattt
     7381 tgattgcagt tgccgcgctg cttctcttta ttatgatttc cgcagggctg tcctcttgcg
     7441 gggctatgtt ttcgggcttg actaatggcg tgcttggcac ttcctacacg tccgaggaca
     7501 gcgacctttt ggcagttgaa aacaactatg ctgcattgga aaacgggctg caaagtgaga
     7561 ttgacaatat cgaaaacacc cacctcggct atgatgaata ccgctatgac cttgacagta
     7621 tcggacataa cccacatgaa cttgcgtctt acttgaccgc cctcttgcag agctacaccc
     7681 cgcagagcgc acaggcagag cttgaacgta tctttgccat gcagtacacc ttgacgctga
     7741 ccgaggaggt tgaaatacgt taccgcaccg aaacaagcac cgacccagaa accgggga
//
LOCUS       DAEMIK010000166         7743 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEMIK010000166 DAEMIK010000000
VERSION     DAEMIK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.169"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            187..1488
                     /locus_tag="KPK87_003312"
     CDS             187..1488
                     /locus_tag="KPK87_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2539427.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1564..1839
                     /locus_tag="KPK87_003313"
     CDS             1564..1839
                     /locus_tag="KPK87_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2539428.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3337..3567
                     /locus_tag="KPK87_003314"
     CDS             3337..3567
                     /locus_tag="KPK87_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2539429.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3560..4141
                     /locus_tag="KPK87_003315"
     CDS             3560..4141
                     /locus_tag="KPK87_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2539430.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4720..5397)
                     /locus_tag="KPK87_003316"
     CDS             complement(4720..5397)
                     /locus_tag="KPK87_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2539431.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5586..6950)
                     /locus_tag="KPK87_003317"
     CDS             complement(5586..6950)
                     /locus_tag="KPK87_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2539432.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINEFCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7078..7599
                     /locus_tag="KPK87_003318"
     CDS             7078..7599
                     /locus_tag="KPK87_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2539433.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3016 a          876 c         1345 g         2506 t
ORIGIN      
        1 ctattatgta gtaaattata taaaacaatg tatcagaata aatagtatat taataatttg
       61 tagtatatca tgacgaataa tacagtcata acaaatagtg agtcataaca aataatgtat
      121 ttataattga taatgtattt aaaaatcaaa atataaaaac ttataaggaa agagagatta
      181 atttatatgg gtaaatcaaa agatgttgaa atagagaagt ctattttagg cacaattctt
      241 ttagacaata aattaagcta taagctagat gaattaaatg aaaacatgtt tatgaatgat
      301 atatgtcttg agatatttaa aattatgaaa gaacttaaaa aagaaaacat agtgattgat
      361 gtggccactg tgaaatcaaa gatagataga aaatctctag caataaaaac aagtgatgtg
      421 actaatctaa ttacttgggg tcagaatttt ggtcttgatg gtcatattaa aattttaaaa
      481 gaaaatttag ctagaagaag cataaatcaa aattgccaaa accttttaca cagcctaaac
      541 cttggagaaa atatcgatac ttgtatttac aaatttgaaa gtaatataaa ggagattttg
      601 gacaaggata cttatgaaaa tgatgatgta aatagtatag cagggaaggt gcttgatttt
      661 ttagaaaaca aaaaagatat tggatttaaa tttggtataa aattacttga taccactata
      721 ggaggtcttt ttaaaggaga gctaaccaca atagcagcta aatcgggggt tgggaaaact
      781 gctttggcac ttcaaataat gttaaattca tttaagcaag gtaaaaaaac tttatttata
      841 agtagagaaa tgaccagtga gcaagtattt atgagaaata tatgcagagt aactggtgta
      901 agtacaagga atatgaaatc taaagaaata gatgaaaatg attggaaact tattgtaaat
      961 gctataggtg atttaagcga aaataactta atttatatta atgataagat agatacaatt
     1021 agtgcaatta gaaagaggat aaggcaggta aagccagatt tattaatagt agattatgtg
     1081 caactcctta catctcagaa aagcatggat aagagagaaa gggaagtagc tactttttcg
     1141 agagaactta aaaatatgac tttagatttt aatatacctg taatccaatt aagtcagtta
     1201 aatgatgaaa tgaaagattc aagaccttat ggagatagac ctatgaggga tagcaaagcc
     1261 atctaccacg atagcaacaa tgtggtttat atacatcaat taaaggggag tgattatgaa
     1321 gaagcagtga gagatattgg agaaagtgag gaggcagtta gagcatcaga atacagggga
     1381 attaagatgg ttgatttaat agttgcgaag tgtagagatg gacagactag gcataagcat
     1441 ttttgttact ttggggataa gttacatttt caagaactaa attattaggt caataaagta
     1501 gtaataacaa gcttattaaa agtcgggaaa tagcaaatag attataaaat atgaggtgaa
     1561 aatatgttgg aattaagaaa aattatagaa ttacttgaag aacacaagat agaagtaagt
     1621 attccaaagg agttacataa gtatagttta ttggaatacc ttgaattgtg tcaacaaagt
     1681 gggggaacat atttttatat taaaacactg gaaggggctt taaaagaggc tagaaatgag
     1741 cttatgatta aattgtttaa tgggaaaaat tatatggaat tagcgaggat gtttgaaatg
     1801 agtgttatta atgtcaggca tattgttaga aaaaaataga tatttttgga gaatacaagt
     1861 gtgatatact tctgaaatag aagttattat agttgaaata aaattgtggt taataaatta
     1921 aagttgtgta taagtttaag attatagagg agtataagtg tgattaacaa catgattttg
     1981 tgaataaaat tgaggatata tatgagttaa atgtgtgatt aataaggtaa ggttgtgtat
     2041 aagattatga gtatttaaaa ggaggggcta taattaatag tgtagagttg tgtgtttgct
     2101 taagattata taataattta atagattaaa ctatactaat aaggtaaaat cgtgtgttta
     2161 gttaaagctt attataaaat cttgatatgt gattaatgat gcaatatgtg aataaagtta
     2221 agattataat agatttaacc tgtggctaat agaataaaat tgtgaataaa gttaagatta
     2281 taataggttt aatctgtgac taatagagta agagtgtaaa tagagataaa cttcaatatg
     2341 agcttaacct atgattaata gagtaagagt gtaaatagag ataaacttca atatgagctt
     2401 aacctatgat taatagagta agagtgtaaa tagagataaa cttcaatgtg ggtttaactt
     2461 gtggttaata gagtaaggtg taactaaaga taaacttcaa tatgagttta acttgtgatt
     2521 aatagagtaa ggtgcaacta aagataaact tcaatatgag cttaacttgt gattaataga
     2581 gtaagaatgt aaatagagat aaacttcaat atgggcttaa cttgtggtta atagagtaag
     2641 agtgtaacta aagataaact tcaatatggg tttaacttgt ggttaataga gtaagaatgt
     2701 aaatagagat aaacttcaat atgagcttaa cttgtgatta atagagtaag agtgtaaata
     2761 gagataaact tcaatatggg cttaacttgt gattaataga gtaagagtgt aactaaagat
     2821 aaacttcaat atgagcttaa cctatgatta atagagtaag aatgtaaata gagataaact
     2881 tcaatatgag tttaacttgt ggctaataga gtaagagtgt aaatagagat aaacttcaat
     2941 atgggtttaa cctgtggtta atagagtagg gtgtaactaa agataaactt caatatgagt
     3001 ttaacttgtg gctaatagaa taagattgtg aataaagtta tagttttaga tgggtattaa
     3061 cctgttacta atagaatatg attaggtgta aaattataga gctgtataag aaaatattat
     3121 agatatatta aatattagat aataaatatt tatatagata cacaaaagaa aaggcgatat
     3181 gaagctaaac ttaatgactt catatcgttt tttatttgaa actcctttgt atttagagtg
     3241 tgatttttaa tattgaggtt ttatgtgatt aagtcacaga taaaaagtaa gtatcttgat
     3301 ataattctta tagattaata atatagggag gttattatgt ctaataaaaa aaagaagtat
     3361 gcaaatattt tagcaaatga gtgcgttgct tgtggtagct gtataaaagc atgtccaaga
     3421 agtgctatct cagttccatg tggcattagt gcaaaaatta atagagattt atgtgttgga
     3481 tgtgggattt gtgaaaagat atgtccagca tctgtaattg agattataac aatattaaaa
     3541 gaggaagaag gtaagtgtca tgaataataa aaaacattgg tatgactatt tatggatttt
     3601 ttcttcaata tatttgatat tgggatttgt aaatattatt ttcgcttgga ttgggctaat
     3661 ttgtttcttt gttccactag ctatttctat tgttaaggga aataaagctt actgtaataa
     3721 gtattgtgga agagggcaac tgtttaattt gcttggaaat aaattgaagc tgtcaagaaa
     3781 tagagatatt cctaaattta ttagaagtaa gtggtttaga tatgggtttt tgacattttt
     3841 tatggtaatg tttgccaata tgcttttttc aacttattta gtgtttgaag gaagtagaaa
     3901 ttttaaagaa gtagtgactt tgttgtggac tataaaaatg ccttggcaat gggcgtatca
     3961 tggaacttta gtttcacctt gggtggtaca gtttgcattt ggtttttata gtgttatgct
     4021 tacatcaact gttcttggtt tgattactat gatattgttt aaacctcgtt catggtgtgt
     4081 ttattgccct atgggtacta tgactcagat gatatgtaag gcaaagagta agtctgtata
     4141 gaaaggcctt atgtgatatt tacagttttg ttcaagtaga gatgagtagt aggtttgaat
     4201 atatatgaca aatatatgag tataggggca tagatttagt ttgtgtaagg taaactgatt
     4261 tatgcttctt ttttatgtct tagattattg gctaagtgga aacaatgaat ttagatatta
     4321 agaattattc agttatttga agaaaataga ataataatta acattatatt aatggatatt
     4381 cattacatat aagccagtta atagcaagat ttatttaata tgaaataggt caagtattta
     4441 aactaaataa ggtcatgaca attaatataa gtatagatat aaaaagatta gaagttataa
     4501 cttataaaag gtattctata taaatagtat ggggaaaata gtcaaattaa cttattagaa
     4561 acaaaaaata tacataaaaa ataaatgtaa atatcaaata agatactata tcaaaataga
     4621 tataagtatc ttttttatta ttgaaattat gaaaatatat ttgatttcac acaaaaaaga
     4681 gtgcctcatg aactaaaaag ttcattttga gacacacttt tacagtgtca aagaacgaag
     4741 tttatcctta tccaatactt ttataccacc acgagataaa gacacaatac cttctctagc
     4801 aaaatatttc aacattctcg atacaacttc tctggcacta cccatatact tagcaatttg
     4861 ctcatgagtc atatttatag tgctactgcc atttttagct atctcatcaa ttaaaaaaga
     4921 tgccaaacgc ttatcaaaac tcataaaaag aatttgttgc atagcccaca taacatcaga
     4981 aaaacgttcg gtagctaatt tatatgaaaa taactctgca tgaatatttt ctgtagataa
     5041 ttttgcaaaa acagaggaac taatctgtat aatatcgcag tccgtttcag catctacata
     5101 gacatcaaaa gttattgttt ttagaataca ggaagctgat aaaatacata catctccatc
     5161 atcaagtcgg aacagtgtaa cctctcgacc ttcatctgaa agtatgtatg tacgcactgt
     5221 tccagacttt ataatcaaaa tgccaataca gtcactatca ccacaatgta ctggattccc
     5281 ttttttgtaa tgtgaggtat ttgctgaaga aattaataat tctttttgta agtcagtaag
     5341 tttatcccaa aaaggcaagc tatgtgtaag tatatcgatt tcattttgtg aaatcataca
     5401 atatctcctt tcttaaattg ttataatccc atgatacaag tgaatagata aattgacaag
     5461 tagtttgtta ataggattat ttagtcagat agtaatagta tgatttatgt agaaaaaata
     5521 tgataatatg tattcttgct attaatgaga aagtaatatg ttttgatgat agtaggtttg
     5581 tataactaat gcataccgtt tatttttaaa agattaagtt ccttatgtaa ccttatagac
     5641 ataaaaccag caattatagc agccatcaaa tcagctattg gttgtgcata taagatacca
     5701 tttaacccat agaatacagg aataatcaaa attataggga taaaacagac accttgtcta
     5761 cacataccta aaaatccacc ttcctttgct tttcctagtg cgaggaataa tgtagaatac
     5821 actgtataga atccaaatag cataaatgaa aacccatttg ccctaagagc tttactacca
     5881 ttttctatta ataaaatgtc accacttgta aattgtgaaa ttatagattt tgggaagagt
     5941 atcaatataa gaccaagcac tacacaaaat actgtagtcc ataatatgga agtttttatt
     6001 gctatatgta atctatcgta gttctttgaa ccaaaattat aaccagcaat tggttggaaa
     6061 ccttttataa aaccaaatac aacaagacta cctaaagacg ttattcgtgt tacagcaccc
     6121 attccagcta tcacagaatc accatacttt gtagcctcca tattagttaa tgtaatggca
     6181 agactagtca ataattgaaa tattaaggta ggtatgccaa tttttaggat ttctgaaaaa
     6241 atttcttttg aaaaacagaa ttcgttaatt ctaaaactaa atatactttt cttgcttaaa
     6301 atatatccta cataaacaag tgttgaagca atctgtgaga tagctgttgc aatagaagca
     6361 ccccttaccc ccagaccaaa agtataaatg aatatcgggt ctaaaatcat attaagcaaa
     6421 gcacctaata acatagcaca catagttgtt ttagcagctc cttcacttgt gacaatatta
     6481 gtcatagtga cattgaatat attaaaaata gatgacacga cataaatgct agcataagat
     6541 attgcataag gcataatact ttttgtagct cctaataatt ttaaaattgg attgagaaaa
     6601 atcataatca caataataac taatgtaccc actataatac tactgtaaat agatgtactt
     6661 accacttttc ctgctttttc attatcacca tggcctaaaa gtctggcaac atatgaagca
     6721 gctccatttc caaacaataa tcctaatccc acaacaagct gacctaatgg aaatgcaacg
     6781 gatatagctc ccatttgact tgttccaagt ccacccacaa agtatgcatc aaccaaattg
     6841 tataatgcag ttatcatcat acctatcatg gtaggtaatc ccatagataa caatgctttt
     6901 gagattgggg cagaccctaa taattcaatt cttttgttac tttctttcat gcttaacact
     6961 cctcttctat tgacattaag tctaaattat aatactataa attatagtaa gtatggaaaa
     7021 tagtactata atttatagta tttgtcaata gtaagaaaac taaaggagat gatttttatg
     7081 gctacaatag atttaattgt attgggaata gtaaagcaac aacccaagag tgcttatgat
     7141 attcaaaagg cagtggaata caggaatata tcacgttggg ttaaaataag tacaccatca
     7201 atttataaaa aagtaattca attagaacaa aagggatata ttgaaagtaa aactgtgaag
     7261 aatggaaaaa tgccagaaaa agcagtttat tcattaactg aattaggtga gacacatttt
     7321 ttagatttaa tgtttgatac atctagcaaa ccaataaata ttcttttaga ttttaatgct
     7381 gttatagtaa atttaaatag ccttacacca gaaaaacaga aattatgtct caataatatt
     7441 gaagagaatg ttcaagagtt aaagaaaact attgaggaaa atataagtat aaagacagaa
     7501 gtaccagaga cagcaaaagc agtgttggag caacagttgg tgctagcaca agcattggaa
     7561 acctggataa attctttaaa agataatatg ggaaattaga attagagaag ctaacttaat
     7621 aatcattaaa caaaaaccag catatttaat aatgagatat tagaaaaaat aaaacaaatg
     7681 atataaactg aaaagtatat ttaaaataaa gctcacttaa ttgagggaga atgttaaact
     7741 ttt
//
LOCUS       DAEMIK010000167         7657 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEMIK010000167 DAEMIK010000000
VERSION     DAEMIK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.170"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            940..2298
                     /locus_tag="KPK87_003319"
     CDS             940..2298
                     /locus_tag="KPK87_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG2539434.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2405..3199
                     /locus_tag="KPK87_003320"
     CDS             2405..3199
                     /locus_tag="KPK87_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2539435.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASTLVKRMYNAIEEGMYND"
     gene            3192..5498
                     /locus_tag="KPK87_003321"
     CDS             3192..5498
                     /locus_tag="KPK87_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2539436.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5637..6914
                     /locus_tag="KPK87_003322"
     CDS             5637..6914
                     /locus_tag="KPK87_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2539437.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7097..>7657
                     /gene="yqeC"
                     /locus_tag="KPK87_003323"
     CDS             7097..>7657
                     /gene="yqeC"
                     /locus_tag="KPK87_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG2539438.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGL"
BASE COUNT         2999 a          837 c         1434 g         2387 t
ORIGIN      
        1 tgttgcagat acaaatatag taaaggagag tacattggta caactgagat acatagctta
       61 ttaagttttt ctgtcttatg agaattatat tataaaatat tcataaatat tttgagttta
      121 aattaaataa ttattttatg cttagattaa ttgatattta attaatttaa taacaaaaac
      181 agtgaaagag taaataatat tatttacagg gggtaaagca aagtatgtta aataaattgt
      241 acttttggcc ccgtacatag agaaatctat gtaggacaca actttaaaac cagtaaatcc
      301 taaagccttg caactacaat aaaagataga aagaaacttt tatgaatgtg gtgaaaacca
      361 gaaacaatag tgaggatggt atatggtgag agcctaagta cttaaaaatg gacatttggt
      421 cgccaagcct agaaatagga aggtcaaacg actatctcga gagagagtac actataagcg
      481 attgatagtg gaaacgggtt gctcttagca ggtaatgctg aagatgaaga aatagtctac
      541 gctcatatga aagtatgagg tcactggatt tgaagtgaca tctataaagt agcgtttata
      601 ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa aacttataac ttataatgat
      661 taagattgaa agatttaaat tgaattaaat ttactataaa aaattaaaaa ttcatagcta
      721 taatactctt atatttataa agcatggtat ataatgtata caaaattaaa gaatacgtgt
      781 atttgttaat aattaaattt attgcatctg ttttttaaga ctaaaaactt aaatatatgt
      841 tggagtatag ttgatattta tgaacatcat taatttaata tattagattt ttaagtattt
      901 atataattca ataagtttta tgtaacggga gtttttatta tgaaagcaaa aagtacatca
      961 aaatttgaat tagacggaat accaccacta aaggaagcaa tacctttagg gttacaacac
     1021 ttaatggcaa tggtaatagg tagttgttta ccagcgatat tgattgcaaa tgcatctgga
     1081 cttaatcata agatgtcaac attattgatt caagccgcat tagtttttgc aggattatca
     1141 actttattac aattatatcc aatacctttg tttaaaggtt ttaaattagg aggaggaata
     1201 ccagttataa tgggagctac agctatgttt ataggagctg gggtatctgt tgcaagtaag
     1261 tatggactac cagtattatt tggctctcaa ataatagctg gtatagtaat aattataatt
     1321 ggttttggtc ttaaaaaaat acgatttata tttactccag ctgtatcagg aacaatagtt
     1381 gcatgtatgg gtcttggact ttttccaata gctataaaaa acttagctgg gggtatgggc
     1441 aatgagactt ttgggaatcc aattaatttt atagttggaa tatctgtatc tcttatgata
     1501 ttaggattaa ataaatatgg aaaaggatta tttaaagatg catcaatatt agttagcata
     1561 atttttggat acattttatc attaattttg ggtatagtaa atttttcttc aattcaggag
     1621 tttactttgg ttgcacttcc aaaaccactt gcttttgggc tagatataag acttgaagtt
     1681 gttgtaatgt tttcaataat atatttagtt gagatagctg atataatggg tgcttgtaca
     1741 ctttctgcgg taggagggct taatagacaa gtaacagatg aagaactttc ttctgctgtt
     1801 ttaggaaatg gagtactctc tacaataggt gcattatttt ctgcaactcc aatggggatg
     1861 tttggacaaa attctgcaat cgtttccaat acaaaggttg taagtaaatt tgtactagct
     1921 attggaggaa ttggattatt tttggcagga atatcgcctt tattagctaa tttaataaga
     1981 acaattcctc cctgtgtggt tggaggagca acactcgtta tattttcaac tcttacaaca
     2041 tctggtttaa gacttgttag tatggatgga tttaatcaag aaaatagcat gatattaggt
     2101 ctttctatgg cttctggtat tggattcatg gtagctcctc aagttcttga aaaatttcca
     2161 aaatttatag agacattact tgcagattca agtgtagttt caggagcaat ggtagccata
     2221 ataattcaag ctttatatat ggtaaaattt cctggaaata aaagtgataa ggttgaggat
     2281 aaaaataaaa gtttataatg taaaggttta atatatgtga attaaatata ctacaattat
     2341 aaatataaat aaaagattta ttaaaaactt ttgtgtttta ataagttaat gatgggggga
     2401 taagatggtt actataaagg aatattcagt tccacagagt ttagaagaag catataaaat
     2461 tttaatttct aaaaaaaata atgttatttt aggtggatgt ggatttataa aattaagtaa
     2521 taaaaatata ggaacagcga tagatttaaa agatataaat ctaaattata taaaagagga
     2581 tgaaaaaaat attttgatag gtgcagatac ttcacttaga agtttagaaa ttgataaaac
     2641 tataaaaaat tactgtagtg gaatactttc aagcgctgtt tctaatatag ttggtgttca
     2701 atttagacaa ggggctagaa ttggtgcaag tgtattttca aagtatggct tttctgactt
     2761 aataccagcg cttttggttg ttggagcgaa ggtgagatta tacaataaag gaatactaga
     2821 gttaagtgag tttttggata gtgaattaag tagggatata ttggtcgaag taatactacc
     2881 aaaaaaagat gcaatagctg tttttgactc aataagaaaa tgcacaggag atttttctgt
     2941 attaaattca gcaatgctaa aagaaaatga tacttacaaa atagcaattg gggcaagacc
     3001 tcaaaaagct agattagcac ttgaagcgag taatatatta aataaagaaa aagatataga
     3061 taaggcatct attgttgcta gtaaggaact tacttatgga agtaatatga gagctagtaa
     3121 agagtacaga aaggatatgg caagtacatt agttaaaagg atgtataatg ctattgagga
     3181 gggaatgtat aatgattaat aaggggataa gaaagataga tagtacagca attgtcacag
     3241 gaaaacctct atatactgaa gacctcatta tgcataaaga tgttttgaca ttaaaactac
     3301 ttagaagtcc acatgctcat gctaagataa agaatataga tacatcaaaa gccgaaaaaa
     3361 ttccaggggt tgtgggtatt tatacgtata aagatgtacc acaggtcaga tattgtgttc
     3421 caggtcaaac agaaccagaa gcatcacctt atgatagagt tttacttgaa gatacagtta
     3481 gatttattgg agatgaagta gctttagtgg ctgctgaaga tgagaaaaca gcacttaaag
     3541 ccttaaaaat gataaaggta gattatgagg ttttaaagcc tgtgtttgat gcaagagaag
     3601 cagaaggaaa tgaagttgtt atacatccag aaaaagacat agtagagcca tatccttttg
     3661 gattggatgc aaaaaataat atagtatcaa aagaattttt tgaatttgga gatgttgaag
     3721 ctaagcttaa agaaagtgat tttgtggttg aatcttcata caaaacccaa agtcaatcgc
     3781 attgcatgat ggaaactcat aggtcatatt catataaaga tataaatgat agaattgttg
     3841 tagtttcatc attacaatca ccacacaatg taaagagaat acttagaaaa gtattgggta
     3901 taccatcttc aaaaataaga gtaattaaac ctagaatagg tggtggattt ggaggaaaga
     3961 atatagcagc aacagatata tttactgcat ttgttactat gaaaactggt agaccagcaa
     4021 aatttgtatt tacaagaagt gaaacttttg taggttctaa tagtagacat gaaatgtttt
     4081 ttgatgttaa agttggtgca gataaagaag gaaatttaaa agcaatagag ctaaagtcat
     4141 taaacaatac aggtgcttat ggtgggaatg gtgtctctgt ttcttccgaa tctggacaca
     4201 ataccttacc aatatataat gatattgatg ctgttagatt taatgctaga acagtatata
     4261 ctaataggtt accagcagga gcacttagag ggtatggtgc tacacaggga atatttgcaa
     4321 tagatagtgc tattgatgaa ttggcacata agattggaat ggatccactt gaatttagaa
     4381 ttaaaaatat aatagatagg tataccaatg gaaaagcagc tgaagacgaa atattaagtt
     4441 gtgagctgag acaatgtata gaaaagggca agaaattaat taattgggac gataaatatc
     4501 catgtaaaga tataggaaac aacaaagtaa gagcagtagg tatggcagta gcaaatcatg
     4561 gttctggtat accgcgtgta gatatggcaa cagtcactat aagaatggat gaagatggca
     4621 catataaact attatctggt cttgctgact taggtcaagg ggctgataca attcaagctc
     4681 aaattgctgc ggaagcttta aatactactg tagatagaat ttctatttat acaggtgatt
     4741 cagacttatg tccctatgat acaggagcat ttgcttcttg tacgacaacg gttacaggaa
     4801 atgcaacaat aaagacagca aataaattaa aagatatatt actttcagtt gcagagtcaa
     4861 agctaggatt taaaaaagaa gatttagagt tgggaaaaga cagggttttt gttaaaaacg
     4921 ataatagcaa gtttgttcta ttagaggaat tgggtagaga atctgttgga ggaataggat
     4981 ttgctagtga tgatgtacca gtagttttgg aagcaacaga atcttttggc atgttgaaaa
     5041 aaataaaacc atttatagca ggatttgcag agatagaatt agataaaaat acaggcgaat
     5101 ataaagtcat aaattttgca tgtgtacctg attgtggaac agttataaat ccaactttag
     5161 ctagaataca agtagaaggt ggtgtgatga tgggtatagg gtttgcaatg tatgaagacc
     5221 ctatatttga taaagatggt agattattaa cagatagttt cctacagtat aaagttccta
     5281 caaaaaaaga agttggaaat atattggtag attttgctga gaatttcgac ccaggaggtc
     5341 cttatggtgc taaatcatta ggagaaatag ttatacatac accagcacca gctatagcaa
     5401 atgcaatatt taatgctaca ggagttcgtt taagagagct tcctatgact tttgaaaaag
     5461 tttatatggg tatgaaaaat aataataaac aattataata aataataagg tgtataaaag
     5521 caagtaaatt taaattattt tgtaaaataa agtaattaaa tggacattat atatggtgtt
     5581 ttgtaaaaat accgtatata atgtctagta gagtatattc taaaggaggt gagttcatgt
     5641 caaacagaat aaaaaatata cactatggaa ctcagagaca acaattaaaa aacttaacta
     5701 aaaaagaata tcaagctcta cgagagatgt gcttcttagc taagaactta tataatgttg
     5761 gtacatataa tgtaagacag cattatttca atcaaggaga acatcttaac tatgaagcta
     5821 attatcatgt atgcaaagaa aatgagaact ataagcttct caatagtaat atagcgcaac
     5881 aaatattaaa agaagtagac agttcgttta ggtcattttt tggattaatt aaattagcca
     5941 aagaaggaaa atatgacttt agagggataa aatcacctaa atatttagat aaagaaggat
     6001 tttttagcat aataataggt caaataagaa tcaaagaaga tgggatttta aatgtaccta
     6061 tgtctccagc atttaaaaaa ctatatggga aagtaagtat aaaagttcca caaaatatac
     6121 tagagaaaaa aattaaagaa ataagaataa tacctaaaca taaagctagg ttctttgaaa
     6181 ttcagtattg ttatgaaata ccaaaatcag aggaagtttc aaatcaaaaa aatgcactag
     6241 ctatagactt gggtttagaa aatttatgta catgtgttac caatttaggt gatagtttta
     6301 tcatagatgg aaaaaggcta aaatctgtga atcagtgggc aaacaaacag aatgataaaa
     6361 tagaagcctt aaaattaaaa aagaatataa ctgcaataac aagaaaacaa tttaaagttt
     6421 ggaataagag aaataatcaa gttaaagact atgtaaataa aacctgtttt tatataattg
     6481 attattgtgt aaaaaataat attggaaatt taattgtagg atataatgct aagttagaaa
     6541 aaagtgataa tatggagaga aaaatatata gaaattttat aagaatccca tttggagaga
     6601 taaaaggaaa attagaatat ctgtgtaaaa taaaaaatat caattttatt aggcaagaag
     6661 aaagctatac tagtaaatca gatttttttg ctgatgattt tctacctgat atcaatgtgg
     6721 acaatgttaa aaaatatgac tttaaagggg aaagaataac gcgtggacag tacaaatcca
     6781 gtgttggaat cattataaat gcagatgtaa atgcgagtct taatatacta aaaaaatcac
     6841 aagtagttaa tttatctaat ttacagaata atcctcaaat attaaagcaa ccacagagag
     6901 taagaatatc ttaattatat ttaaaagata aactgatagc gtaagcttac agcttagcgg
     6961 cgaattggat ccgccttgtt gtttacaaca gaatctattt actttcgagt ttatggaatt
     7021 tcaaaaatca cttttactaa aaaggtctaa ctataataat taagagatta gaaaactggt
     7081 tataagaggt gaaagtgtgg aactatcaaa tataatagaa aagaatgaaa taataactgt
     7141 agttggagca ggaggaaaaa cttcgtttat aaactatttt gcaaattttt atagagataa
     7201 attgaaagtt cttcttacaa caacaactaa aatatatgta ccaaacgatt atgacaatat
     7261 aattattaca atagatggaa ctgttattcc atctatttgt catggaataa cagtatgtgg
     7321 tagttatata aataatgaaa ataaattagt aagtattgat tcctctattt tggatgaaat
     7381 tgtagaccaa tttgatttag ttttaataga gggagatggc tcaaagagaa aaaaacttaa
     7441 aggttggaat gctaaagaac cagtagtata tcataaaact acaaaaacca tagggatatt
     7501 agatataaca tcatttggaa tgaatattaa tgaagagaat atacatagag tagaaatatt
     7561 taaaaaaata gcaaatttag atacaagtag tattaatagt agttctacag taagtattga
     7621 aaacttaaaa aatatagtat taaatccaaa tggatta
//
LOCUS       DAEMIK010000168         7547 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEMIK010000168 DAEMIK010000000
VERSION     DAEMIK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.171"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            227..718
                     /locus_tag="KPK87_003324"
     CDS             227..718
                     /locus_tag="KPK87_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2539439.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            991..1662
                     /locus_tag="KPK87_003325"
     CDS             991..1662
                     /locus_tag="KPK87_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG2539440.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            1747..2613
                     /locus_tag="KPK87_003326"
     CDS             1747..2613
                     /locus_tag="KPK87_003326"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG2539441.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            2646..4148
                     /locus_tag="KPK87_003327"
     CDS             2646..4148
                     /locus_tag="KPK87_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG2539442.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            4278..4730
                     /locus_tag="KPK87_003328"
     CDS             4278..4730
                     /locus_tag="KPK87_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG2539443.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            4890..5693
                     /locus_tag="KPK87_003329"
     CDS             4890..5693
                     /locus_tag="KPK87_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539444.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDAIDLSEARKYICELEKISKRFV"
     gene            5751..6638
                     /locus_tag="KPK87_003330"
     CDS             5751..6638
                     /locus_tag="KPK87_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2539445.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            6657..7505
                     /locus_tag="KPK87_003331"
     CDS             6657..7505
                     /locus_tag="KPK87_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2539446.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
BASE COUNT         3032 a          718 c         1387 g         2410 t
ORIGIN      
        1 atttagtata tattatgcat ttttgatggg aatattggct ttatttagta catttatact
       61 agatagtaat attaatttct ttaatacaac taattatgga acaactttaa taatgcaatt
      121 taaagtctta caagctattg taatttcatt tgtatatagt tttgtgatat ctttaatagg
      181 atataagcta aattcagctg attaaatatg attggaggga atttctgtgg aaggaaatac
      241 taataataaa gtagatgata aattttctaa ggttaagaaa aagtttggta gtggaaaatt
      301 taaagttgaa ttatataaag aaaaatcaaa attaaataga gagattgatg atattcaaga
      361 aaagaaagca aagatattct tggaaatggg aatactgaca tatcaaaaaa ttagagaaga
      421 ttctattaat gataatagtt ttgataaatt ctgtgatgag ttactagaac ttgataagtt
      481 aatttatgaa aaaaatatgg aaattaatga aatggaaatg gcagaaagta atgtaacttg
      541 tgagtgtggt tatgttggaa atgtaaatga taaatattgt gctgaatgtg gtaaaaaatt
      601 tgaattggaa aatgaatgtg atgattttat aatttgtggt tattgtgaaa gtgagataga
      661 tacagaggct gaattttgtc catgctgtgg aagaaaaatt ataatggatt tagaataata
      721 ttgggaaacc tatgtatagt atacatgggt ttttttattg aaattattta aatggactta
      781 taacagtagt tatataaaat gaaaataaac accaaaaaaa tgaaataaaa tttcttttaa
      841 atgtaaaaaa caagctaata tataaaataa atcagtaaaa atatagatgc gagaaaaaaa
      901 tatgaaaaaa tattgcaaag aaaaagtttt catgttatta ttaatacata aatcaagaaa
      961 ataaatttca gaaacgaggt aatttaaaaa atgctagata aggtaaaggg aagattaata
     1021 gtatcatgcc aagctttaga agatgaacct cttcatagtc cttttataat ggggagaatg
     1081 gctaaggctg ctatggaagg tggagctgtt ggaattagag ctcaaggagt tgaagatatt
     1141 attgaaataa aaaaagttac tggacttcct gttataggta ttataaagag aaactatgaa
     1201 gattcagata tatatataac gccaactaaa aaagaagttg atgaactttt aacaacaggg
     1261 tgtgaaatga tagcattaga tgctaccaat agagttagac caaataatga agacttaaaa
     1321 gaacttataa agtatataaa agaaaacggt gttctagtaa tggcagatat ttcaaattat
     1381 gatgaggcta tcaaagcaca agagtacggt gtagattgcg tttcaactac actatctgga
     1441 tatactccat atactaaaac attagaaggt ccagattttg tattaatgga aagattagtt
     1501 aaagatttag aaatacctgt aatagctgaa ggaaaggtaa atactcctca agatttaaaa
     1561 aaggtctttg agttgggtgt tcattcatca gtagtaggtt cagcaataac aagaccacaa
     1621 ttaataacag aaaaatttgt taaagcaatt gaaattaatt aaaaattaat ttataaatta
     1681 aaaaattatt atatttaact atatttgaaa attaaaattg gaggtaaaga tatgagaaca
     1741 acagatatga agggaattta ttcagcatta ttagtttcat ttgataagga aggaaatata
     1801 aatgaaaaag ggttaagaca aataattaga cacaatatag atgtttgtaa agtagatggt
     1861 ctttatgtag gaggaagtac aggggaaaac ttcatgcttt caactgatga aaagaaaaga
     1921 atatttgaaa tagctaaaga tgaagtgaaa gaagaaataa aattaatagc tcaagttggt
     1981 tcagttaatt taaaagaagc tgtagaactt gctaaattta caactgattt aggatatgat
     2041 gcaataagtg ctgttacacc tttttactat aaatttgatt ttgaagaaat aaaacactat
     2101 tataatacaa taataaattc tgtagataat agattaataa tatactctat accattttta
     2161 actggtgttg atatgagttt agaccagttt ggagaattat ttgaaaatga aaaaataata
     2221 ggtgttaagt ttacagcagc agatttctac ttattagaga gaatgagaaa aacattccca
     2281 aataaattaa tatttgcagg atttgatgaa atgatgttac cagctacagt tttaggtgta
     2341 gatggggcaa ttggttctac attcaatgta aacggtgtca gagctagaca aatattcgaa
     2401 ttaactaaga atgaaaaaat atcagaagca cttgaagtac aacatgtgac taatgattta
     2461 ataacagata tattaggaaa tggcttatat caaacaatta aattactact tgaagaacaa
     2521 ggtgttgagg ctggatactg tagacaacct atgaaagaag caactgatga aatgaaatca
     2581 agagcaaaag aaatatacag aaagtatttt taataaaata cttttagtat gggaggatta
     2641 gtcaaatggt aggatttact tggatagatt ttgcaatact agtggtttac ttattggcag
     2701 ttttatttgc aggattgtta ttttcaaaaa aggaaatgaa gggaaaagag ttcttcaaag
     2761 gtgatggaac tattccgtgg tgggttacat cagtatcaat atttgccaca ttattaagtc
     2821 caatatcatt tttatcatta gcaggaaatt cttatggtgg tacttggatt ttgtggtttg
     2881 cacaattagg tatgtttata gcaatacctt tgacaataaa atttttctta cctctatata
     2941 gtaggttaga aatagataca gcatatgaat acttagaaat gagatacgaa agcaaaggtc
     3001 ttagagtact tggagctttg atgtttataa tatatcaaat aggacgtatg tctatcataa
     3061 tgtatcttcc atcaatagtt ctggctggtc ttacaggaat acctgtaaat gtactgataa
     3121 tagttatggg tgtaatagca ataatttact catatacagg tggattaaag gctgtcttgt
     3181 ggaccgattt tatacaagga atggtattaa tagtaggtgt tacagggaca ttgttctatt
     3241 taatagcaag tataaatggt ggttttggaa cagtaatgga tactcttaca actggacaca
     3301 agttcttagc aagtaatgaa gttatgtttg ataaaaatat actatctaca agtgcattta
     3361 taatatttgt tggagctggt cttaatactt tttcatcata tgtatcgagt caagatatag
     3421 ttcaaaggtt tactacaaca actgatataa agcaacttaa taaaatgaca tatggaaatg
     3481 gtgttttatc aatgggactt gcaacagtat tttatctaat tggaacttgt ttatttatat
     3541 actatactca aaacccagat ttagctcaaa cagttcaaca agaccaagta tttgcatcat
     3601 atatagctta tgaattacca gtgggtataa caggaatact acttgcagcg atatatgcag
     3661 cttctcaatc aactttatcg acagggctta attcagttgc tacaagttgg actttagata
     3721 tacaaactat cataacaaaa gatataagta tggaaaagca aactaagata gcacaatata
     3781 tatcattagg tgtaggtata ctttctatag ctgttgcaat agtattagca aatggagaaa
     3841 taaagtcagc atatgaatgg tttaatagtt ttatggggct agtactaggt gttttagcag
     3901 gaatatttgt acttggtgca ttttgtaaaa aagcgactaa aatgggagca tatgttggat
     3961 tcatagtatc agcaatttta gttgtttatt taaaataccg tatgccagaa gtaacttctt
     4021 gggcatattc tctaataaca atcacgactt ctgtagtggt aggtacaata gttagtgcaa
     4081 tagaagctaa agttaaagca aaagtatctt taccaaaagc tgagactact gtttattatg
     4141 aaaaataata tttaaatgat aaattgtctt atagagtgat tagataaata atttagttac
     4201 tctataagat tgtataatat actttgtata taaaagtata aaaatagact ttataattat
     4261 atagggagat agtgcttatg atatttggaa atataaatca tagtaagaca tattcaacat
     4321 tgcatgaaga tttacttaga tgttttgaat atgctaaaga taattcatta atagattatg
     4381 aaaaaggtac ttatacaata gatggtgatg atatttttgt gaatattgta gagtataaaa
     4441 cttgtgaaaa agaagataga ttctgggaag ctcataagaa gtatatagat gttcatttga
     4501 tgcttgatgg atgtgaacga atagatataa attttataga aaatttagaa caaaaaatat
     4561 atgaagaaaa gggcgatttc ttgcctcttg atggtaaaaa taatggatac atggaactta
     4621 gaaaaggtga ttttttagtt tgttatccgg aagatgctca tatgacagga ataaaagttt
     4681 tagaaaaaga aaatatcaaa aaagctattt ttaaggtttt ggttagataa tgataattta
     4741 tataaccact agggctgaaa atagtgattt aactttcagc gattagtgag acttcgtaaa
     4801 ttttagttag attactattt tcagccttag ttttttatga gaaaattgaa aataattaaa
     4861 agcttaaaat tttgtaaagg agttaactta tggaaaatat agatgtattc ttattaatag
     4921 gacaaagcaa tgctagagga ttgggaaatc caaaagagag catcattcca aatgaaaatt
     4981 gttttgaata tttgagtact gatgaaatta taaatatgag atgtgagcta gagacttcag
     5041 aaggtgatgg tactatagca cctgcatttt caaatgaatg gaataagctt actggaaata
     5101 aagtttgttt tattcacaat gcaaaagatg gttcaagaat aaaaaactgg aatcatgata
     5161 ataactggtt tctaaatgac actatagaaa aatttaatac aggctgtact accttaagta
     5221 aacattataa tatagaaaat aaatacgtta tttggattca aggtgaaagt gatgcaaaat
     5281 atggtagtga tgtattgtac tataaagaaa gtctaaaaaa aattgcgtat aggcttaaag
     5341 aagaatgtat gattgataaa atgtttgtaa gtttaactgg ttattggctt ggagaagatg
     5401 aatattttat tagaacaaga cgaattgcag cagcacagga gtcggcttgt agtgaatgtg
     5461 atatattatg tgtaggaagc aaaattgcta tgcagtttca tgacagagga ttaactattg
     5521 atgatgttca ttacactcaa gaaggtctta atatgttagg agaggactta tgtaaaaata
     5581 tttataagta tcatataacc aaagaaaaaa gtgtattaag tgatgctata gacttatcag
     5641 aagcaaggaa atatatctgt gaattggaaa agataagtaa aagatttgtg taatataaaa
     5701 ttttaaatag agttgttaat ttataaaaat aatttgtatg gagatatgtt atgaaaaatt
     5761 acgtttgtat agatgttgga ggtacttcaa taaaatatgg ttttattaga gaagatggat
     5821 ttataattga taaatcaagt cttgatactg aagcaaagga aaaaggtgga gaaggaatat
     5881 tagccaaaat aaaagatata gtaaaaaaat atatagagga aaatgaaata agtggtatat
     5941 gtatatcaac tgcaggtatg gttgacccag ttgaaggtaa aatactattt gcattagaag
     6001 aattaattcc aaactataag ggcatgcaat taaaaaaaga agtagaaaaa gagtttaata
     6061 taaaatgtga agttgaaaat gatgttaatt gtgctggact tggtgaaatg tggcttggag
     6121 caggtagagg tgcaacatct tctatatgtt taacaatagg tactggaatt ggtggatgta
     6181 ttataataaa taataaactg ataaatgggt ttagtaatag tgctggtgaa gtaggatata
     6241 tgaatgtaaa tggaagttct tttcaagaat tggcatctac tagcagtcta attaaaaaag
     6301 tagcaaaaat aaaaaatttg aatgaaaatg acttaaatgg aaaaataatt tttgatttag
     6361 caaagaataa tgatgaagat tgtttaaaag aactcgataa tatgataaaa tcattagcag
     6421 ttggaatcgc aaatatatgt tatgtaataa atccagaagt ggtaatttta ggtggtggga
     6481 ttatggcaca agaaaagttc ctaaaaccaa aaatagacaa agcattaaag gaggttctta
     6541 tagagagagt ttacaaaaac actaatatag aatttgcaaa gagacaaaat gatgcaggaa
     6601 tgcttggagc attatataat tttttaaaca aaatataaag aatagaaggg tttttaatga
     6661 gtattttaga acaattggaa tcaccaactt ttaaagtcac aaaatcagat aaattattaa
     6721 tagcttatat aaaagaaaat atagatgatg tattttataa accaatatca caaatagcta
     6781 aagagagcaa tattggtgaa gcaactataa ctaggtttgt aaaaaaaatg aattttaatg
     6841 gattacagga ttttaaagtt actttagcac aagaaatatc aactatcaat aaaaaaaata
     6901 ttataaataa aaatatacaa aatgatgagc cagctttgga tacagcaaaa aaacttttaa
     6961 gttcaaatgt aaccacttta gagaacacag tagagataat caatagtaag gatgtacatg
     7021 actgcgcaag gcttataata aatgcaaaga aagtttactt cataggtatt ggatattctg
     7081 gtataatagc acaggattca aactataaat ttatgagaat aggtcttaat tgtgtaagtt
     7141 ttgacagtag tcatacaatg ataatgatgt cttccataat ggaagaagga gatttaataa
     7201 ttgcaatttc acattctggt gaaacagaag aaattataaa aactgtaaag cttgcaagag
     7261 ccaataatgc taaaattata tcaattactg aaaataaaaa ttctgaatta aaggatattt
     7321 cagatgtaca cttatcttat gtatcgggag aaacagtact ggaaaccggt tcaatatctt
     7381 ctaaattagc acaatttttc attattgatt tagtatatac acaggtagta aaagaacttt
     7441 ctaatgaagc tattgaaagg aaaattaaaa ctacaaatgc tataaaattg tttaaaaatg
     7501 aataaaataa gaaatttgta atgttttata gagctctctt aaaaatt
//
LOCUS       DAEMIK010000169         7524 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEMIK010000169 DAEMIK010000000
VERSION     DAEMIK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.172"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1295)
                     /locus_tag="KPK87_003332"
     CDS             complement(<1..1295)
                     /locus_tag="KPK87_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBG2539447.1"
                     /translation="MTRDGLIEVNETQQTAERISSREADSDFSQPDSAAAERVIEHLD
                     AAHTRKENKKEVKKEQEATALRTSTSRLQFTDEERATPELETYIKKSEKAADRLDAAK
                     AAIPKQKKLVKERTFDEATGKAKTRLRFEEQEKPVPGGKKGNPLSRPAQEAGIFVHNK
                     IHSVEKDNSGVEGAHKSEELAERGAKYGARKLKQGYRSHKLKPYREAAKAEKAAFKAN
                     VNFQYHKTLHDNPQLTSNPLSRFMQKQQIKRQYAKAAKKGGAKAAAEATRKTAKKTAE
                     ETRKAAAFAARHPAGVLIAVAALLLFIMISAGLSSCGAMFSGLTNGVLGTSYTSEDSD
                     LVATEQNYAAMENALQSEIDAIESTHPDYDEYRYDLDSIGHNPHELASYLTALLQSYT
                     PQSAQAELERIFAMQYTLTLTEEVEIRYRTETSTDPETG"
     gene            complement(1322..2281)
                     /locus_tag="KPK87_003333"
     CDS             complement(1322..2281)
                     /locus_tag="KPK87_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBG2539448.1"
                     /translation="MKTDVIINRDALAALKELPSESVHCCVTSPPYYALRDYGLDAQI
                     GQEDTPEQYIDRLTAVFGELYRVLRKDGTLWLNIADTYCGTGNKGGYADPKNPKGRTG
                     QRIARNSRVTGCKQKDLIGIPWLLAFSLREQGWYLRSDIIWQKQNPMPESCKDRPTRC
                     YEHIFLLSKEKKYYYDAAAIAEPLAPTTAERYRRARSTNSKYTQEIPGQGKVQGLNRP
                     RDSGYYDDALMPTTRNKRDVWLINTVPYKGAHFAAFPPKLAETCILAGCPKGGIVIDP
                     FFGSGTTGFAAKSLDRHYIGIELNAEYCALARARIGGAGLSSN"
     gene            complement(2278..4707)
                     /locus_tag="KPK87_003334"
     CDS             complement(2278..4707)
                     /locus_tag="KPK87_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2539449.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAEAIRKAKGDGKARTAQ
                     QTIPYLAMYPDGICKVTEKRYSKCVMFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     SVQLSFINQGTQREQAEKAISIPAQEDAFNSIRTEYSDMLKNQLSKGNNGLVKHKYIT
                     FTVEADNKAAAKSRLSRIETDVLNNFKVLGVTARPLSGYERLKVLHGVFHPEGEPFSF
                     SFDWLTPSGLSTKDFIAPSSFRFGEGRYFRMGKKIGAASFLEILAPELNDRMLADILD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GSEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCRLTRLDY
                     QQEAGLLSSVPIGENLIPIQRGLTTSSTAIFIPFITQELFQTGQALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREMTNAFLITDDDIIICDPEAEYFSLVQRLN
                     GQVIRLSPTGRGIDGKPQYVNPMDINLNYSEDDNPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRPFLADPDPAKMPILGDLYDELLRQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELSNRLVCFDIKQLGKQLKKLGMLIVQDQTWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNVKDLLASREVENIFENSDFVL
                     MLNQAQGDRTILAKQLNISPQQMKYVTHTEAGEGLIFYGNVVLPFVDRFPKDTELYRV
                     MTTKPEEVSSL"
     gene            complement(4655..5059)
                     /locus_tag="KPK87_003335"
     CDS             complement(4655..5059)
                     /locus_tag="KPK87_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG2539450.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQIVCFAAALLIGLPLFFLLK
                     GSAGTNLAAMAMIVVMLPCFLLAMYEKHGQPLEVVVRNVIQTKFTRPKERPYRTENLY
                     AVLEKQRNLEKEVSAIVKRTNKKDAGSRRKQA"
     gene            complement(5081..5950)
                     /locus_tag="KPK87_003336"
     CDS             complement(5081..5950)
                     /locus_tag="KPK87_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539451.1"
                     /translation="MQSILDAINEWIKEILIGAINGNLSTMFGDVNEKVGTIANEVGQ
                     TPQGWNANIFNMIQTLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     IFKAFVAVFIVTNTFNITMAVFDMAQHIVSGAAGVIGGDTNIDVAEALAAMQEGLEDM
                     EIPELLLLVLETSLVSLCMKIMSVLITVILYGRMIEIYLYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVNDMIIADNLHSAVFSLAAYTVILCFSLF
                     KSGTLAKSIFNAH"
     gene            complement(5967..6182)
                     /locus_tag="KPK87_003337"
     CDS             complement(5967..6182)
                     /locus_tag="KPK87_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG2539452.1"
                     /translation="MEFFNSAIGVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            complement(6381..6548)
                     /locus_tag="KPK87_003338"
     CDS             complement(6381..6548)
                     /locus_tag="KPK87_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539453.1"
                     /translation="MNRRIKKKKDKKITEAINGLVSIAERREQQRQAAIRQFVKRCEA
                     LYEQQRKERRN"
     gene            complement(6541..>7524)
                     /locus_tag="KPK87_003339"
     CDS             complement(6541..>7524)
                     /locus_tag="KPK87_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2539454.1"
                     /translation="TLLEMINASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKK
                     FLLSAGKTRSSILISCGARLAPFDIKELRDLMETDEMELDTIGDRKTALFVIISDTDD
                     TFNFVVSILYTQLFNLLCDKADDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRS
                     REISASIILQSQSQLKAIYKDNADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDS
                     FNTSENRGREVSHGLNYQKLGKQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKH
                     PNYKYLSDYDKKNTFDMEKHLRRRPALVKPDEVFDYYEISESDLQEDTDHE"
BASE COUNT         1609 a         1895 c         1874 g         2146 t
ORIGIN      
        1 tccccggttt ccgggtcggt gcttgtttcg gtgcggtagc gtatctccac ttcctcggtc
       61 agcgtcaagg tgtactgcat ggcaaagata cgttcaagct ctgcctgtgc gctctgcggg
      121 gtgtagctct gcaaaagggc ggtcaagtac gacgctaatt cgtgggggtt atgcccgata
      181 ctgtcaaggt catagcggta ttcatcatag tcggggtggg tgctttcgat agcgtcaatt
      241 tcactttgca gggcattttc catagcggcg tagttttgtt ccgttgccac aaggtcgctg
      301 tcctcggacg tgtaggaagt gccaagcaca ccgttagtca agccggaaaa catagccccg
      361 caagaggaca gccctgcgga aatcataata aagagaagca gcgcggcaac tgcaatcaaa
      421 acacccgccg ggtgtcttgc cgcaaatgcg gcggctttcc ttgtttcctc ggcggttttc
      481 ttcgctgtct ttcttgtggc ttccgctgcg gcttttgccc ctcctttctt tgccgccttt
      541 gcatactggc gttttatctg ctgtttctgc atgaaacggg aaagcgggtt agaagtaagc
      601 tgcgggttgt cgtgcagggt cttgtgatac tggaagttga cgtttgcctt aaatgctgcc
      661 ttttctgcct ttgctgcttc cctgtatggt ttcagcttat ggctgcggta gccctgtttt
      721 aacttcctcg ccccgtattt cgcgccgcgc tcggcaagtt cctccgattt atgcgctccc
      781 tccacgccgg aattgtcctt ttcaaccgaa tgtatcttgt tgtggacgaa aatccccgct
      841 tcctgtgcgg ggcgggacag agggtttccc tttttgccgc cgggtaccgg tttttcctgt
      901 tcttcaaagc gtaagcgggt ctttgccttg cctgtggctt cgtcaaaggt gcgctcctta
      961 accagtttct tttgcttcgg gatagctgcc tttgccgcgt ccagtcggtc agcggctttt
     1021 tccgattttt tgatataagt ttccagttcc ggcgttgccc gttcctcgtc ggtaaactgt
     1081 aagcgggaag tggaagtacg caaagcagta gcttcctgct cttttttgac ttcctttttg
     1141 ttttcctttc gggtatgtgc tgcgtcaaga tgttctatga cgcgttccgc tgcggcactg
     1201 tccggctgtg aaaagtcgct gtctgcttcc cggctgctga tacgctcggc ggtctgctgt
     1261 gtttcgttta cctcgataag cccgtcgcgg gtcattttct gtgtgatttt atcgcgtggt
     1321 tttaattgct tgatagtcct gcacctccaa tccgcgccct cgcaagggcg caatactctg
     1381 catttaattc aatcccgata taatggcggt caaggctttt tgctgcaaat cctgtggttc
     1441 cgcttccgaa aaacgggtct atgacaatcc cacctttggg acagcccgcc aagatacagg
     1501 tttccgctaa tttcggggga aaagctgcaa aatgcgcccc tttatatggg acagtgttaa
     1561 tcagccacac gtcccgcttg ttccgcgtag tcggcatgag cgcgtcgtca tagtagccgc
     1621 tgtcccgcgg tcggtttagt ccctgtacct ttccctgtcc ggggatttct tgtgtgtatt
     1681 tgctgttggt gctgcgcgct ctgcgatacc gttctgcggt agtaggtgct aagggttccg
     1741 caatggcggc agcgtcatag taatatttct tttcctttga aagcagaaag atatgttcat
     1801 agcagcgggt agggcggtct ttgcagcttt ccggcatagg attttgtttt tgccatataa
     1861 tatcgctccg tagataccac ccctgttcac gcagggaaaa cgccaagagc cagggaatac
     1921 cgattaagtc cttttgcttg cagccggtaa cgcggctgtt ccttgcaatc cgctgtccgg
     1981 tgcgtccctt tgggtttttc gggtcagcat agccgccttt atttcctgtg ccgcaatagg
     2041 tgtctgcaat attcagccat aacgtaccgt cctttctaag cacccggtaa agctcgccaa
     2101 aaacagcggt cagcctgtca atatactgtt ccggcgtgtc ctcctgtccg atttgtgcgt
     2161 caagcccata gtcccgtagc gcatagtacg gcgggcttgt aacgcagcaa tggacgcttt
     2221 cagagggcag ttcttttaag gcggcaagcg cgtccctgtt gataatcaca tctgttttca
     2281 taggctgctc acttcctccg gcttcgtcgt cattaccctg taaagctcgg tatctttcgg
     2341 gaagcggtct acaaaaggca gcaccacatt tccataaaag ataagcccct cgcccgcttc
     2401 ggtgtgggta acgtatttca tctgctgcgg ggaaatatta agctgctttg caaggatagt
     2461 acggtcgccc tgcgcttgat tgagcataag gacaaaatca ctgttttcaa agatattttc
     2521 cacctcgcgg cttgccaaaa ggtctttgac gttctgcgta atggctgttg ggataccgcc
     2581 ccatttacgg aaacgcttcc aaatctccac gctgtaagcg gcggtctgtt cctcttttaa
     2641 gagcaaatga aactcgtcca tatagtagcg ggtggatttc ttttctgccc ggtttatcgt
     2701 taccctgttc catgtctggt cttgcacaat gagcatacct aactttttga gctgcttccc
     2761 aagctgcttt atatcaaagc agacaaggcg gttagaaagt tccacgttgg ttctgtggtt
     2821 gaatacgtta aggctgccgg aaacatacag ctccaatgcc gccgcgatac gggcagcttc
     2881 cggctccggc tgtcttagca gctcgtcgta aaggtcgccc aagataggca tttttgccgg
     2941 gtcggggtct gcaaggaacg ggcggtacac atttcttaca gccctgtcaa tgacggtctt
     3001 atcgacaggc tgcaagccct ccttgccgcc aatgacaagc tcgcagaggg aaaggataaa
     3061 atcacttttc aacgcaagag ggttgtcgtc ctcgctgtaa ttaaggttta tatccatagg
     3121 gtttacatac tggggcttgc cgtcaatgcc cctgcccgta ggggaaagcc gtatcacttg
     3181 cccgtttaga cgctgcacaa gggaaaaata ctcggcttcc gggtcgcaga tgataatgtc
     3241 gtcgtcagta atgaggaaag catttgtcat ttcccttttg gcggcgaaac tcttaccgct
     3301 tcccggcgtt cccaagataa gcccgtttgg gtttttgagc tgctttctgt cgcagagtat
     3361 catgttgtta gaaagggcgt tcaagccata gtacagggct tgccccgtct gaaataactc
     3421 ctgcgtgata aagggaatga aaatagcggt gcttgatgtg gtaagccccc gctgtatcgg
     3481 gataaggttt tccccaatcg gcacactgga taaaagcccc gcttcctgct gatagtcaag
     3541 gcgtgtaagg cggcagttgt atttctgtgc gatacccgcc gccgcgaaaa tgtcattttc
     3601 cagtttccgc ttcgtgtctg ccatgtttac cacaaggaac gtaaggagaa acattcgctc
     3661 gttgcggctc tgtaagtcct gcaacaagtt ctttgcttcg ctgccaaagg tggcaaggtc
     3721 ggacgggatt atatccatgt catagccgct tctaaccgct tttttctgtt cctcaatctt
     3781 catcttgtca aggtctgtga ttttgcgctt aatggtcttg attgcttccg actggtcgat
     3841 actgcggata tgcaagttga cgattacgcc cgtttccaag tccagtatgt cggcaagcat
     3901 acggtcgttg agttccggcg caaggatttc aaggaagctc gccgcgccga ttttcttccc
     3961 catgcggaaa tatctgccct cgccaaaacg gaaagaggac ggggcgataa aatcctttgt
     4021 agacagcccc gacggggtaa gccagtcaaa ggaaaaggag aacggctcgc cctccgggtg
     4081 aaatacccca tgcagcacct ttaagcgttc atagccggat aaggggcggg cagttacgcc
     4141 aagcaccttg aaattgttaa gcacgtcggt ttcgatacgg gaaagccttg actttgcagc
     4201 cgccttgtta tcggcttcaa cggtaaaggt aatgtatttg tgcttcacaa gcccgttgtt
     4261 gcctttacta agctgatttt tcagcatatc cgaatactcg gtgcggatag aattgaaagc
     4321 gtcctcctgc gcggggatag agattgcttt ttctgcctgt tcccgctgcg tcccttgatt
     4381 gatgaaagaa agctgcacgc ttacgctcgc gtcaaagtag ttgaggaaat cgcaccagtt
     4441 ttcaaaaatg gcggtcttat cgtcagcctg tgcaagctga taattgatgt cctcgaacat
     4501 gacgcacttt gaatatcttt tttccgtaac cttgcagata ccgtccgggt acatggcaag
     4561 gtagggaatg gtctgctgtg cggtgcgggc tttgccgtcg ccttttgcct ttcggatagc
     4621 ttcggcaatc tgtttctttt cggcgcgggt cagcttacgc ctgtttcctg cggcttcccg
     4681 cgtctttttt gtttgtcctt ttgacaatcg ctgatacctc cttttctaag tttcgctgct
     4741 tttccaagac agcgtatagg ttttcggttc tgtaaggtcg ctcctttggg cgggtaaact
     4801 ttgtctgaat gacgtttctt accaccactt caaggggctg tccatgcttt tcatacatgg
     4861 caagcaggaa gcaggggagc atgacgacaa tcatcgccat agccgcaagg tttgtccctg
     4921 cgctgccttt gagcaaaaaa aacagcggca gtccgattaa gagagccgcc gcaaaacaaa
     4981 cgatttgccg ctttgtcaga ttgaaagcga cttttgtttt gactttggat aggtcttttg
     5041 gtacaggtac atacgccaag aaaaaacctc ctttctgccc ttagtgggcg ttgaatatgg
     5101 attttgccag tgtgccggat ttgaacaggg aaaaacagag gataactgta taggcggcaa
     5161 gggaaaatac cgcgctgtgc agattgtctg cgattatcat gtcgtttacc agtacggcgt
     5221 aaataccgac acatatcata atgaggaagc cttgaaaacc aagagcgaac agccctttta
     5281 ggtagttgtt cccaatctgt ccccattctc tgttggtcat agttgcaaat ggaataggcg
     5341 ataccgaaca gtaaaggtaa atctctatca ttctgccgta gaggataacg gttatcagta
     5401 cggacatgat tttcatgcaa aggctcacaa ggcttgtttc aagcacaagt aagagcagtt
     5461 cggggatttc catatcttca agcccctcct gcatggcggc aagggcttcg gcaacgtcga
     5521 tattcgtatc gccgccgata acccccgctg cgccggaaac gatatgctgc gccatatcaa
     5581 aaaccgccat tgtgatgttg aatgtgttgg ttacgataaa caccgcaaca aaggctttga
     5641 atatccactt aaagaacatg aatgtttcta tgtcgtgcat attgttcttt tccgttacca
     5701 tgctgataag ctcgtagcat aggacatagg taatgacaag ccccgcaatc gggacgatta
     5761 cattttcaga aagggtctgt atcatattga aaatgtttgc gttccaaccc tgcggggtct
     5821 gtcctacctc gtttgcgata gttccgacct tttcgtttac gtccccgaac atagttgaca
     5881 gattaccgtt tatcgctccg ataaggattt cctttatcca ttcgttaatc gcgtcaagta
     5941 tgctctgcat aggcagttac ccgcgcttaa ccgaacaagc cggaaagcaa aggtacaagg
     6001 atagtgccga taagggcaac gccgccgccc gccatgagct gtttaatgcc ctgtgatttt
     6061 gcacccggat tgtcgtttcc atatccctct aagagattga taacgcccca aatgccaagt
     6121 cctgctccaa gagcgataac caaagtctgt aatacgccga ttgcgctgtt gaaaaattcc
     6181 ataaaactcc tttctgccgc aaatgcggct tgccccgtaa aagggcataa aaaacggcgg
     6241 tcagttttca aactgccgcg gtcataagtc cccgcgctgc ctaaatgtct gccgcgcggc
     6301 ggtattcagt tgtcagtgtg gcgataggaa tttttgtagg ggcgcgtatc atgcaagttg
     6361 tctttttctt tgttactgcg tcaattcctc ctttcttttc gctgctgctc atacagggct
     6421 tcacaccgtt tcacaaactg ccggatagca gcttgccgct gctgttcccg ccgttcagca
     6481 atagaaacaa gcccgtttat ggcttctgtg attttcttgt cctttttctt tttgatacgt
     6541 ctattcatgg tcggtgtcct cctgcaaatc gctttcgctg atttcgtagt agtcaaatac
     6601 ttcgtccggc ttcacaaggg cgggtctgcg ccttaaatgc ttttccatat caaaagtatt
     6661 tttcttgtca tagtcggaaa gatatttata gttggggtgc tttgtaatgt cgtacttatc
     6721 agagaaaaac gggcgcaccc ctcgtagctg taaaatacat ttcccgccgt ccataaccgc
     6781 tatttcatct tccgtcataa gctgcttgcc taacttctga tagttcagcc catgcgatac
     6841 ctcgcgcccc cggttctcgg aagtgttgaa gctgtcaatg gtttctttcc ccaagatttc
     6901 cgacatttct ttgagggtgg ttttctcctt gccgcccaag aaaagggtgg tgtcgcagtt
     6961 gccgactatg gtatcggcgt tgtccttata gattgccttt aactggcttt gcgactgtaa
     7021 gataatcgac gcggagattt cccggcttcg tatggtggct atgagctttt caaacttcgg
     7081 tatctgcccg atatttgcaa attcgtctaa cagacagcgc acatggacgg gcagcctgcc
     7141 gccgtattca tcatctgcct tgtcgcaaag gagattgaaa agctgcgtgt aaagaatact
     7201 cacaacaaag ttaaaagtat cgtcggtgtc gctgatgata acaaacaggg cggtcttgcg
     7261 gtcgcctatg gtgtcaagct ccatttcgtc ggtttccata aggtcgcgca gctccttaat
     7321 gtcaaaaggg gcaagccgcg caccgcagga aatgaggata gagcttcgtg tctttcccgc
     7381 agataacagg aatttcttgt actgccggac ggcaaagtgt tccgggtctt tttcctccaa
     7441 ccgttcaaac atgaggtcaa cgggggactg aaattccggg tcgtcctcgc gtgcttcact
     7501 ggcatttatc atttcaagca gcgt
//
LOCUS       DAEMIK010000170         7490 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEMIK010000170 DAEMIK010000000
VERSION     DAEMIK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.173"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            15..326
                     /locus_tag="KPK87_003340"
     CDS             15..326
                     /locus_tag="KPK87_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG2539455.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      419..511
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            611..1405
                     /gene="thiD"
                     /locus_tag="KPK87_003341"
     CDS             611..1405
                     /gene="thiD"
                     /locus_tag="KPK87_003341"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG2539456.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1429..2217
                     /gene="thiM"
                     /locus_tag="KPK87_003342"
     CDS             1429..2217
                     /gene="thiM"
                     /locus_tag="KPK87_003342"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG2539457.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2240..2872
                     /gene="thiE"
                     /locus_tag="KPK87_003343"
     CDS             2240..2872
                     /gene="thiE"
                     /locus_tag="KPK87_003343"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG2539458.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGKNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDIPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3011..3313
                     /locus_tag="KPK87_003344"
     CDS             3011..3313
                     /locus_tag="KPK87_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2539459.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3379..4128)
                     /locus_tag="KPK87_003345"
     CDS             complement(3379..4128)
                     /locus_tag="KPK87_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539460.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4122..5084)
                     /locus_tag="KPK87_003346"
     CDS             complement(4122..5084)
                     /locus_tag="KPK87_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539461.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5088..5546)
                     /locus_tag="KPK87_003347"
     CDS             complement(5088..5546)
                     /locus_tag="KPK87_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2539462.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5902..6258
                     /locus_tag="KPK87_003348"
     CDS             5902..6258
                     /locus_tag="KPK87_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2539463.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6330..7046
                     /locus_tag="KPK87_003349"
     CDS             6330..7046
                     /locus_tag="KPK87_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539464.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7039..>7490
                     /locus_tag="KPK87_003350"
     CDS             7039..>7490
                     /locus_tag="KPK87_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539465.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRN"
BASE COUNT         2928 a          870 c         1239 g         2453 t
ORIGIN      
        1 ggatgtgatt taaggtgtat tttacatata ttataagatg taaagatgat tctctataca
       61 ctggatatac cagtaatata gttagaagaa tgaatgaaca taaattagga attaattcaa
      121 aatacacaag agcaaaggga tttgaaaaac tagaagttta ttttgtgacc aacacaaaaa
      181 gcaatgctat gaagttagaa tactatataa aaaaattaac tagaaataaa aaattgtcta
      241 taataaaaaa cccaagtata cttataaatt taattgataa taaagaagat tatattattg
      301 gtaaggaaat agaacaatta acataaatat ttatagtaac attataatgg gtgcatatgg
      361 tgtaaaaact tgacaataaa tactattgta atataatgaa gttataaata tagtaaatta
      421 aaatagctag gggagccaag agctgagagg tataccgacc cttacacctg atctggataa
      481 taccagcgta ggaaagctta gtaaaaattc aagtatgtat tatttgtgat atatgctatt
      541 tctaagctat acctattgta ggtatagctt ttttaattaa gttatttaaa ttaaacttta
      601 ggaggcaaca atgagtaatt ataaaatacc aacacttaca atagccggct ctgattcatc
      661 tggaggagct gggattcagg ctgatttgaa aacgtttagt gcaattggta cttatggtat
      721 gagtgtaata acagctataa cagcacagaa tactaaagga gtttttgctg tagaggattt
      781 aaataagaaa ataataaaaa aacaaataga ggcagtcttt gaagatatac ctccaagagc
      841 tgtaaaaata ggaatggttt caagtcctga gattatactt gaaatagttg agaatctaaa
      901 gaaatacaat ccaaaatatt tggttgtgga cccagtaatg atatctaaaa gtggatatta
      961 tctattaaag ccagaggcaa aagaaaactt aattaagtat ttgataccac ttgcatacat
     1021 aattacacca aatataccag aggcagaaga aataacagga ataaaaatac ataatgtaga
     1081 tgatatgaag agagtaggag aagaaatact acaacttgga cctaaatttg tacttatgaa
     1141 aggaggtcat ttagatggag aagcagtgga tattttagtt ggtaaaaata tatttaaagt
     1201 ttataaaagt gaaagaattg ataaaaaaaa tacacatgga acagggtgca cattatcttc
     1261 tgcaataaca tcttatctag cattaggata tgaaataaca gaagcagtaa atctaagtaa
     1321 aatctatata acagaagcaa taaaaagaag ttttgatata ggtcatggag taggaccagt
     1381 acatcatttt tataaatttg agtaataaaa aaatattgga ggtagaatat gtataattta
     1441 attaaagatg taaaaaaatt aaatccattg gtaattcatt atactaataa tgtaactata
     1501 aatgattgtg ctaatgtaac tttagctgta ggagcaagtc ctcttatgag tttttcatat
     1561 gaggaagtag aagaaatggt aagtgtagca aactctgtag taataaatat aggaactatg
     1621 aactcaaata tgttggattt attcctatta gctggaaaag cagcaaataa gtacaataaa
     1681 cctgttgttt tggacccagt tggagtattt gcaagcaaag cgagagcaga acttacaagt
     1741 agacttttaa atgaagtcaa atttagtgtt gttaaaggga atgtatcaga aataaagttt
     1801 ataggtggct ttaatgtaag gggtaaaggt gtagattcat ttgatgaaga agaagattct
     1861 actgagataa taagaaagat tgctgaaaag cttgaatgtg tagttgtagc aactggaaaa
     1921 atagatataa taactaatgg taaaggtaca tataaaataa acaatggaac tgataaatta
     1981 aaaggaatta caggaactgg atgtatgact gctagcttga ttgcaagttt tatggctgtt
     2041 actgagaata tattagaagc agcaacaatg ggtgttctta caatgtcttt aagtggggaa
     2101 ttagctaatt taaataatcc accaataggt acatttaaag aaaatcttat gaatgctatt
     2161 tatcaaatgg atatagatac tttaagtaaa aattctaata ttgaattttt aaattaataa
     2221 agagtaaggg agacttaaaa tgattgataa agaatcttta aaaaaatgtt taaagttgta
     2281 tttagtaact gactctgaaa tgcttaaagg tagagacttt tataaatgtc ttgaagatgc
     2341 aatatcttct ggaataacaa ctgtacaact tagagaaaaa aatgcttctg gaagagagtt
     2401 tttaagaaaa gctatgaaac ttagagaaat aactaaaaga tatggtgtta agtttataat
     2461 aaatgataga gtggatatag cacttatttg tgatgcagat ggagtacatg taggacagag
     2521 tgacattgat gttagagagg ttagaaagct tataggaaaa aataaaattc taggagtatc
     2581 agcaagaact ttagaagaag ctatttgtgc aaaaaatgat ggcgcagact atttaggtgt
     2641 agggtctata ttcacaacat ctactaaatt agatgcaaaa tcagcttcgt ttgaaactgt
     2701 taaagaaatt aaagaaaaag tagacattcc atttgtacta attggaggta taaatttaga
     2761 caatatagat aaacttaaat gtttagaaag tgacgggtat gctataatat cagctatatt
     2821 aaaagctgaa gatatctcta aagaagtgga aaagtggaca ttaaaaatat aaaattggct
     2881 ctggtagtga aaaaaatgga aaaatttgat ataatctaag agtaaaattt aagcaattat
     2941 aaatgataaa cttttctaca tatacttttt aatcaagata tatgtagatt tggttaaaaa
     3001 ggagctataa atgattagga tattgttagt gtgtgttgga ggcatgtctt ctaccctatt
     3061 ggtaaataaa atggagaagg atgcaaaaaa aagaaatata gactgtaaaa tatgggcagt
     3121 tggagaagga gatataaaat cggagcttga taactttgat attttacttc ttggaccaca
     3181 acttagattt atgcttgatg acgtaaagtc tatagttgga gatagagctc cagtatctat
     3241 aatagatatg gtaaactatg gcacttgtaa tggtcatgct gtgttaaatt cggttttaga
     3301 aatattaaaa taaataataa aagtggattc tatataatag agtccatttt tattatttgt
     3361 tattaatagg cttaaaactt agcttaccat ttcttttttt aatctccaca tagcaagtga
     3421 aaaacttgaa cagcacaata ttaaaaatag taggataaca ggcagtatat ctccaataag
     3481 aatagtctca ccaaatatta agctacgtaa agcgttaata acatgggtaa atggattgag
     3541 catcactgct acttttaatc ctccagataa actttctata gggaaaagag ctgaacttaa
     3601 aaagaaaata ggaagtacaa ttgcagtcat aattgtttca taaactattt catttggcaa
     3661 taataaactt atagaataag ctagacttga taaaaagaag gctgtcataa atatcagtac
     3721 tatcataagt aggattccac caatgccaga ttctatcctt actgagaaga atatacttac
     3781 tatacacaaa atagttactt ctatgaaaga aactaagata gcttctaata attgaccaag
     3841 gacgattgaa tacctgctga taggtgcaat taagactcta tagaaacttc cactgttctt
     3901 cattataaag tttataatac caccactact acaagaagaa aacacaacca gtacaatgat
     3961 acctggtagt ataaaagcag tataattatt gatatttata ttattcatag tttggtttgc
     4021 aatagaacta taaagcacaa gccaaagaaa aggttgtaat atagtaacta caaatgagat
     4081 tggattttta aaacgccact tcatactacg ccataagata tttaacattc ccattcctcc
     4141 tttaagcttt ttttagaact tgtaagtgct aaaaatacat cttcgagact tggctctaca
     4201 atttctattg catcaaattc tatatcatgt tctaaaagcc atttatttat taaagtaaaa
     4261 gcagttctac tatcgtttac acttataaaa attgaatttt ctcgtacgct cataaacttt
     4321 actaaaccag tacttttaat tgaatcttta tattttttta tatcttcagt attatgaaaa
     4381 gttatacgta gtatattttg tctaatgtaa ctacgtaaac ttgatggtgt tccttgtgct
     4441 aaatcctttc cattcttcat aatacaaata ttatcactca attgttcagc ttcttcaaga
     4501 taatgagtag ttagaaatat agttgtgcca tactcatctc taatttttaa taacatatcc
     4561 cacatagatt ttcttgaatc gacatccata ccaacagttg gttcatctaa aaataggatt
     4621 ttaggagatg ataccatatt catagctatg tccaatctac gttttacacc ccctgaatat
     4681 gatgaagtag gatattttaa gtaactagat aagtcaaact tatcaattaa agagtctatt
     4741 ctttgttttg caatctgtgg ttctacttta tacattttac tttgaaatac catattttcc
     4801 ataagggata aatgttcatc aatagagatt tgctgtgcta cacaagcaat ttgagtacgt
     4861 atccaagaag gattatctac taaatctttt ccaaacattg ttacatttcc agatgtaggt
     4921 ttgtaaaagg tagtcaaaat atttataaga gaagatttac cagcaccatt aggaccaagt
     4981 aaagaaaaaa tttctccagc atttacatta aagcttagac catttaaagc tttcacacca
     5041 tttttatatt cttttactaa attgtctatt tcaatcgcta acatatatta ttcctcctct
     5101 ttaagtggaa atattatctc tgtaatatag ttatctggat ttccttttat caacatacct
     5161 ggtcctttaa taaatacttc tctccaagga gtttgtattt ttatattatt ttcatgaata
     5221 tatttaaata tcatttcata cgtttttggc aaattactat aagagcctgt gtgagtaaaa
     5281 aataaagctt ttgttttagg aaattctttt gtagttatac ccatacgatt tggagtttca
     5341 gcagttggaa cacataattc catttctgca acaccagttt tttggtcaac atcatagtag
     5401 caaaagaatg gtgcaccgtt agactttcct tttatagctt taaatacatt tggaaaatac
     5461 tttgttgcct cagtcatagg tccattataa cgcatggtag ccactgtgac tgattcgata
     5521 tttttgattt caattttgca attcatttgt ttacctccta atgtgtgttc atattcttac
     5581 tagattttta agcattaata catataagat tttgattagt tgaaaacata taatcttata
     5641 tcgtttgctt gttttcaagt ctaacataac ataaaatata tatcctctca tttgatgctc
     5701 cattttgtaa agttattatt taagctttac tttagatttt ttagcaaatt aaaataaagc
     5761 aatttataaa ataagtattt acaaattaag tgtattaatc gtataataca cttaatagga
     5821 tactagataa agtctgaaat aaaggcgtat ttttttaaag taagtgtact atcgtattaa
     5881 tacactagaa taggaggaat catgaatttt gaattagata atacaactcc catttatttg
     5941 caaatagtaa agtatataaa gagacaaata gtaacaggag agttgaaacc aggagagaca
     6001 ataccatcaa gaagagaaat ggcacttaat ctaaaagtaa acttaaatac agttcaaaga
     6061 gcatataagg agatgggtga tatgaatata ataaatactt ttaaaaatta tcaaagcagt
     6121 gtaacagttg atgaaaatat attaaaaaat ttaaaattag agcttataaa tgaatcacta
     6181 tctgttttta tagaagatat gaaagcaata aatgttagca aagaagaagt tttaaaaata
     6241 atagaagata agtattagtt ttttaacaaa tagaaaactt agtttagaaa gtttatttat
     6301 aagcatattg gatgtggagg atagtaaata tgatagaaat aaagaatgtc agcaaaacat
     6361 ataaaaggat gcaagggctt aaaataagaa agatagaagc tttaaaaaat gtaagtttta
     6421 atatagaaaa gggaaaaata actgcattac ttggtataaa tggtgttggt aaatcaacta
     6481 tgttaaaagc aatagcagga cttataaaca ttgatagtgg agaaattcgt atagatggag
     6541 aaaaaataaa tgaaaaggta tacaataaat tggcttttgt tccagatgta caatctcact
     6601 ttagcaatac aacaataaaa gaaacatttg aatttatgga aattttttat tcaaagtgga
     6661 ataaagaaaa atcaaaagaa atgatggaca tatttaaatt ggatgaagat gaaataattg
     6721 ataacttgtc aaaaggaaac attgcaagag taaaattaat acttggattt tgccaagacc
     6781 cagagtacat acttcttgat gaaccattta caggaattga tttgtttaag agggaagagt
     6841 ttataggagt aatagcgcaa tatatggagg aaaatcaagc cattatcata actactcatg
     6901 agatagtaga gatagaaagt ttagttgatg aggttgtaat tcttgatgaa gggcagatta
     6961 taacttcatt taatgcagaa gaattaagag aaagagaagg taaatctata ttagataaga
     7021 tgagggaggt atataagaat gaataaactt ctatatctaa taaatgtaga tttgagaaga
     7081 agcaataagt tttatatagc ttatatatca tttttttcat taattacact tagtttaaat
     7141 ttattcgaaa ttaataaatt taaaaattct aaatttatta tgaacttagc aatagaagat
     7201 tttggaggta tattttatgg actagggata cttagtaatc ttggttttat ccaaagtata
     7261 ttgttctttg ggatattggg actttttata tactatattt ttatgtggaa aagagagttc
     7321 ttttcaaata atcaaagtat ttatacactt atgatgttac ctcaaaataa gtttaagatt
     7381 tgtatatcta aatcaatagt tttgataaca atgatatatg gttttttgat aagtcagaca
     7441 gcaacattat ttatatgtaa atatatattt aatttcattt ttagaaatat
//
LOCUS       DAEMIK010000171         7464 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEMIK010000171 DAEMIK010000000
VERSION     DAEMIK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.174"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(234..1295)
                     /gene="ytvI"
                     /locus_tag="KPK87_003351"
     CDS             complement(234..1295)
                     /gene="ytvI"
                     /locus_tag="KPK87_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2539466.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNALFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            1569..2309
                     /locus_tag="KPK87_003352"
     CDS             1569..2309
                     /locus_tag="KPK87_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG2539467.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            2321..4099
                     /locus_tag="KPK87_003353"
     CDS             2321..4099
                     /locus_tag="KPK87_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2539468.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            4266..5183
                     /gene="pfkB"
                     /locus_tag="KPK87_003354"
     CDS             4266..5183
                     /gene="pfkB"
                     /locus_tag="KPK87_003354"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG2539469.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            5220..7139
                     /locus_tag="KPK87_003355"
     CDS             5220..7139
                     /locus_tag="KPK87_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2539470.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         3054 a          720 c         1288 g         2402 t
ORIGIN      
        1 aagaaaaatt taaaaatata gatgttttaa ataatgaaaa tgaatataaa gaaatgattg
       61 atattatttg tgcagagtag aaaaaaaggg agtgtcttag aatagacttt ttagtttatg
      121 aggcactctt tttgtataaa atcaaatgta ttttcatcat tttaacaatt aaaaagagct
      181 tatccctatt tgagaaagcc ctttttaatt taaataaaaa tgtcaaggtt aaactactta
      241 aatgcagtaa taatcccatt atcatgtaag ttttttatga ttgtagctgc gatagggaat
      301 ccaaataatc cagctattcc aaatagttga gctccaacaa acatacacat taatgttatt
      361 aaaggatata ggccaatttg gtgtcctact acctttggtt ctaatatttg tctaacaacg
      421 gttataatta tgtatagtat taaaagtcct attgctaaat taatatttcc attaatgagt
      481 gagataatca tccaaggtgt caaaatactt cctgtaccca agactggcaa aatatcaaca
      541 agtgctatga tgacagatat agtaaatgca ttttcaacct ttaggatagt aagtccaata
      601 agtagttcta tgaatgtcac tgatagtagt attgcatagg cttttagtag ttttaaaaga
      661 gcattaatac tgctattttt tatatttatt atcataaatt gacttttttc tggaaattgt
      721 tttaagataa agtttactat tttttgatag tcaaaagtaa agaaaaaaga agcaattaaa
      781 gtaataataa gttttaacat aaatgcaggt gttttacttg caatactagc aatagcattg
      841 agtgcatttg tggaaataga agctactaaa gacatcatag attgacttat attatcccaa
      901 ccaagtatta atgctaggtt aacattaggt gtactagcat ctatctttga aaataaagca
      961 tttagcgttg gttgtatact tgatttatat atttcaggaa gtctaaaaaa tacatcactt
     1021 acacttgcaa atatcttcac accaaagcta actaataaaa atatacacaa cccataaaaa
     1081 attaaaagta ttactataga aataagtgta cgatttaaat ttgtattatt tgtaataagt
     1141 cttataatag gtcttaagat ggatgcaatg ataaaactga ttacaaaagg cattaataat
     1201 ggcaataaga attttaatac aatatataca atacttgtta ttaaaactac atatgtaaat
     1261 ttaattataa aattcttttg cttttgaata tccataatat aaaacctcca atgtatttga
     1321 tattattcta aatatggtct tttttacaga aaatatacta ctgtatattg attataaact
     1381 ataaagctta tgtaaagtaa ataaattatg taaaatttag gttaattttg tataaagaga
     1441 tacttttata ttttacaatt aataaccaaa tactaaaaaa tattttaaat aaagaactta
     1501 aaattgtgat aaaattaatt tatagatatt aaaaaaagta taatatatgg tttttaagag
     1561 gtgctaagat gaaaaatttt agagatttag gtgggaacaa aactgaggat ggaagaactg
     1621 taaagaaggg attattttat aggtctgcaa aattatcaaa tctaagtgaa aatgatataa
     1681 agattttaaa agagttaaat attaaatata tatttgatta tagaagtgat gaagaagctc
     1741 gtaaacatcc atcaacaata atatcaaata taaaaaatat aagaatacca gcaatgagag
     1801 agctagaaga atcaggagga agttttggtt ctattgaaga tatgattgat ggattgtttg
     1861 aaaaagatgg tgcatttaat atgttaaata atagttacta taatttgcct ataaataatc
     1921 cgtcatataa aaaattagta gaacttataa gagactattc caatttacca atattaaatc
     1981 attgtacagc tggtaaggat agaactggtg tggggagtgc tataattctt atgattttag
     2041 gggtatcgag agagaatata atgaaagatt atctaaaaag taatgatttt gcagataaag
     2101 agatagaaag atttatagaa tataagccta aatttaagga cattccaaaa gaaaatttaa
     2161 aatatatatt tggtgtaaat gaagagtata tgaaaactgc atttagaaga attgatgaag
     2221 aatacattag tgtagaagca tatttatatg gagaatttaa tttaaataaa gaagaaataa
     2281 gaaaattaag aaatcaatat ctagagtaaa aggtgagttt atgagtttaa atataacagc
     2341 aagacagata aatataataa aaatattgtt aaattcaaaa gatacaatta gtggtattgc
     2401 cttaagtcaa gagataggat gttcttcaaa aactatacaa aatgaaataa aggatattaa
     2461 taaacaactt agaaatggta aaattttgtc aataagagga gttggttata aactagaagg
     2521 aagttttgat gaggtaaatc taaatagcaa tctctatgat gatgtagata gagtagaata
     2581 tataatcaga aaaatattaa ccttaagtaa tgaagaaaaa aatactataa aacttgaaga
     2641 tttagccgat tctatgtatg taagtgtatc aacagtaaaa aatgatttaa aagaagtaaa
     2701 aagaatattg gaaaagtatt caatatcagt taaatctaag cataaacaag gtatatgtgt
     2761 tttggaaagt gaagataaga tattaaattg tatagttgac ttaagcaata agagagataa
     2821 tcaattgagt ttaaatgatt ttttaaatga tgatataaag gaaagaaaac tattaataaa
     2881 aaaattatta ttagacatat taaatgaaaa tggattagta ttaactgata tagaatttaa
     2941 gagtgtgtta aataatatat tagttatgtt aagcagacat aagtataatg aaaaagaatt
     3001 tatagagaat tatatagaaa attataggag caaaagaagc tcaattttag aaaatgaaca
     3061 aaacaaagaa attataataa attcaataaa aacattttgt aaaaatttaa aattagctac
     3121 atcaatagat ataagtaatg ataaggtatt tgaagagttt ttatataagc atattagtag
     3181 tttatgtaaa aaaataaagt taggtataaa tcaatcacct ataatggctc aagatataaa
     3241 aataaaatat ccatttgctt ttgaactagc taatatagct aaaaagacaa tagaaaaaga
     3301 tttgaagata gaaattaatg aagatgaagt tgcaaacata gctctacata taggaggagc
     3361 agttgaaaga gcatcttata ataataaaga taaggttttc aaaactataa tagtttgtac
     3421 ttcaggcata ggcacatcaa tgttaataaa agctaaatta gagaatatat ttaaggaaaa
     3481 attagaaata ctaaagatta ttccttcata cttagttgaa tatataaaag ttatagatgt
     3541 tgattttgta atatctactg ttcctattga agtaggagat gtgccagtaa taaatatatc
     3601 tccaatttta agtgagaaag aaattagatt aattgaaaaa tatatagaaa caggaaatat
     3661 atacctagat ttagatataa aaagcctgtt tgatagtgaa ttatttttta ctgatttggt
     3721 gtttaaaagg aaagaagaag ttatagattt tatggcaagt aaacttgtac aaaagggcta
     3781 tatagatgag gatatgaaaa aatcttactt tgagagagaa aaaatagcaa caacagaaat
     3841 agggaatatg gtaactatac cacatggagc tattgggaaa atttttgata ataaaatagc
     3901 aattggtata ttaaagaagc ctatttcttg ggaaatatca gatgttcgtt taataataat
     3961 gttagccctt gataaagata aaatattaga ttatgaagta atattttcaa aaatatataa
     4021 aagagtagat tctatagcaa aagttataag tatatgtgaa aatagtagtt ttgaaaaatt
     4081 tgtaaattta tttaattaga tgggtagtat tgtatataca cctgtaaatt tggataatat
     4141 aaaactaaat aagataaaaa cgataaattt ctcttttaat taagagaaat tttttgactt
     4201 tcaaggatat taatataaag ttaagatata aacataaagc aaataaataa aaaagaggtg
     4261 gcaatatgat ttatacagtg acattaaatc catcaataga ttatatagtt aagctagatg
     4321 agctaaagac aggaagtaca aatcgtgtaa aagaagaata cgtttatcca ggaggaaaag
     4381 gtataaatgt atctcgcata cttaaggaac ttggaaatga taatacatct ttaggtttta
     4441 taagtggttt tactggagaa tatataataa gaacattgga agaaaaagaa ttgaagacag
     4501 attttataaa gataaaaagt ggtttttcaa gaataaatgt aaaaataaag gcgttaaagg
     4561 aaactgagat taatggacaa ggtccaaata tagatgatga agacattgat actttatata
     4621 aaaaacttga taagctaaat caagatgata ttttaatatt ggcaggaagt ataccatcta
     4681 ctttagatga aaaactatac gaaaatataa tggctagatt agaaaaaaag aatataaaag
     4741 ttgtagtaga tgcaactaaa aatcttcttc ttaatgtttt aaaatacaaa ccttttctaa
     4801 ttaagcctaa taacgacgaa ttagaagaac tatttggtgt taaattaaat tcaattgaag
     4861 acatggtaaa atatgctaga aggttaaagg aaatgggagc tataaatgtc ttggtatcaa
     4921 tgggaaaaga tggagcattg ttgattacag aggaagatga agtgcttata agtgatgttc
     4981 caaaaggaaa agtaaaaaat tctgtaggag caggtgattc aatggtagca ggatttatat
     5041 ctgggtattt gaatactggg aaatatgatt atgctcttaa attgggagca gcttcaggta
     5101 gtgcaactgc tttttcatat gacttagcca aaagagaata cattgataaa ttggtaaatg
     5161 aaatatctgt aaagcagttt taaaatatta aaaaatatat agaaaggtaa ttaatattta
     5221 tgaaaattat agatttatta gatgaaaagt caattaagtt aaatttaaat tcaaaaacca
     5281 aatcagaagc aatagaagaa ttagtagatt tggtcgcaaa tagtggaaat ttaaatgata
     5341 aagaaaacta caaaaaagca attttggctc gtgaagaaat gagtacaaca ggaattggag
     5401 aaggtgtagc tatacctcat gctaaaaatt cttcagttac taaagcttgt atagcagcag
     5461 cagtgtcaaa agaaggtata gattatgaat cgtttgacgg aagtttatca aatctatttt
     5521 ttatgatagc tgcaccagac ggagctaaca atactcatct tgaagtatta tcaagattat
     5581 ctacaatact tatggatgaa gactttagaa ataaacttat aaattcaagc tctgaaaaag
     5641 aatttaagga aataatagat aaaaaggaaa gagaaaaatt cagtgaagaa tatcaagatg
     5701 agaaagttgc tgaaaaaaat ataatagata ctaaagagaa tgatgctaat aagtatccta
     5761 aggttttagc agtaacagca tgtccaactg gaatagcaca tacatttatg gcagctgaaa
     5821 gtttaaataa aatggcagaa aacaaaggtg tatcaataaa agtagaaact aatggttcag
     5881 caggtgttaa aaataagctt acaaaagatg aaatagaaaa tgcaacatgt ataatagtgg
     5941 cagcagataa aaatgttgag atggctagat ttaatggcaa aaaagtcata aaaactaagg
     6001 tggctgatgg tatacacaaa gcggaagaac ttatagacaa ggctgtaaat ggagatgctc
     6061 caatttacca tggaggagat ggaagtcaca acgaatcaaa tgaagaaagt gaaagtggat
     6121 ttagaaaagt atacaaacac ttaatgaatg gtgtatctaa tatgttacca tttgttattg
     6181 gtggagggat acttatagcg atagcctttt tacttgatga ttatactatt aacccttcaa
     6241 actttggttc aaatacacct atagcagcat tttttaaagg aataggagat aaagcttttg
     6301 gatttatgtt acctgtactt gctggatata tagcatactc tatatctgat agaccagcgt
     6361 ttgttgtagg atttgtagga ggtgctctag caggtgatgg tggttcaggc tttttaggtg
     6421 cactacttgc aggttttata gcaggttatt tagtagaagg gcttaagaaa atatttagtg
     6481 tactaccagc ttcattggaa ggaataaaac cagtattgtt atatccttta cttggaacac
     6541 ttttgatggg aattataatg acatttttag taatacctcc agttacagca attaataatg
     6601 caatggttgg atttttaaat ggtttaggtg gaacttcaaa aatagtttta ggattagtat
     6661 taggtggaat gatggctgtg gatatgggtg gacctgtaaa taaagcagca tatgtatttg
     6721 gagttgcatc acttgaatca gggcagtttg aaataatggc agcagtaatg gctggtggta
     6781 tggttccgcc acttgctata gctcttgcaa ctacattttt taagaataga tttactaaag
     6841 aagaaagaga ttctggtaaa gttaattatg taatgggatt atcatttgtt actgaaggag
     6901 ctataccttt tgcagctgga gacccacttc atgttatacc agcttgtgtt ggaggttcag
     6961 cagtggcagg agcacttagt atgttgttca acgcagctct tcgtgcacct catggaggag
     7021 tatttgttat accagtggtt acccatccat ttgcatatat attagctata gctgtaggtg
     7081 ctttggttgg aatgatgctt ttagcattac ttaaaaaacc attaaatcaa gaagtatagc
     7141 aattaaagga tattcaagaa cttaaaatat gatgttttat aaaaattaat atattgctga
     7201 attattttaa aataaggatt ttatgaggct gttatataac gatttgtaat tgttatcttg
     7261 acagtctttt atttttatat aagtaaaata agtgtgtttt gctttatttg tatttaaatt
     7321 gttttcaagt tagaacataa agaaaattat atattatttt aatattcact cgaactataa
     7381 aataatattt gttgtatttt tttgtatata aaatatattg taatattaaa aaaataatag
     7441 taaactgatt gcataaaaat gatt
//
LOCUS       DAEMIK010000172         7460 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEMIK010000172 DAEMIK010000000
VERSION     DAEMIK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.175"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(941..2155)
                     /locus_tag="KPK87_003356"
     CDS             complement(941..2155)
                     /locus_tag="KPK87_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG2539471.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            2592..3308
                     /locus_tag="KPK87_003357"
     CDS             2592..3308
                     /locus_tag="KPK87_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG2539472.1"
                     /translation="MKHIKIAAGLAHVNYGHISAIVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            3443..4069
                     /locus_tag="KPK87_003358"
     CDS             3443..4069
                     /locus_tag="KPK87_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2539473.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(4269..5813)
                     /locus_tag="KPK87_003359"
     CDS             complement(4269..5813)
                     /locus_tag="KPK87_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2539474.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(5839..6480)
                     /locus_tag="KPK87_003360"
     CDS             complement(5839..6480)
                     /locus_tag="KPK87_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG2539475.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGIK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            6750..6941
                     /locus_tag="KPK87_003361"
                     /pseudo
     CDS             6750..6941
                     /locus_tag="KPK87_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         2759 a          964 c         1068 g         2669 t
ORIGIN      
        1 aattttaaga taatgcacta tcaacacact cttaagtttg cttctaagtc ttatttccat
       61 aactttaggg ttaaccatac gcaagaccaa tcactctcgg acaatactca tgattttcgg
      121 taaaaaacgt aaaagaaata agaacaatta acaaaatgta taataacaat caatagcgac
      181 taatgatttt catgtaaaag gacaccaaac tatacggtgt ccttttcaaa ttcttacgtc
      241 atatcaaggc tcattcaatc gtggtaaatt cgtggtaaaa tgagtgcttt cctatatttc
      301 aaaatgcttg aaagtatagt gtttatgcgg ataatcaaaa atcagatata aaatttatta
      361 ataaatatta cattaatcta ttgttctagt taaaatcgta tttcaattaa attcaattaa
      421 aataaataac tctttataaa aagcaattat ttaggtagag gaatatttgc taactttaat
      481 agtctttgtt aactttaatt ttatttaagt tattatagtc ttactaattt tataattgta
      541 gatttaacta ctttatgttc ttttattgtt tatgtagttg atttagaagt tattttaacc
      601 atttatttta attgaagttg tttgactttt aaatcctttg tgtaatcttt gtttttctag
      661 ccatgataaa agtatttcta tttgtatcac tatatataat aatatcatga caaaatattt
      721 attgtctaac taattataat ctatccaaaa tgaaaatggg tgtgagatat tattacaaca
      781 aattgtatta gtattaaaaa gattaaaaaa ttaaaaaact aaacagttaa ataattaaac
      841 gattaagtga ctgtcatata acaatttata attttattta acagccactt aggtatttaa
      901 gagcgataga attgagtgta tatcaaatta taataatttc ctagtaaata attgaaaaat
      961 aaggtagtcc atttttatat ttgatatctg gtttaccttg aataagtgga tatagataat
     1021 ttagggcttc atcagttata taattacatt cttcatttat ccactcttta ggaaaatatt
     1081 ttgtcttgtt agctatttta gaaatatcag tatgactaac ttcatactta taaggattat
     1141 tgcatgttct attaatgata gccataactc ctgtatatcc ttttttagat agttctatcc
     1201 catatgcacc aacttcagct gcttgtttta aatcagtatc tgaagctaaa tgcatagcac
     1261 atctttgtat tacaccaagt tctaaggttt ttactcttgt tgtaattgat gatgatacta
     1321 taagattctt tagatagttg cttactccac ctaattggga atgaccaaat ttatcatgag
     1381 cagaagtttt ttgttcgcat ataaagttgc cgtatttatc ccttatacct tctgatgcaa
     1441 ctatataagc cttatttttt tctttaaata tttttctaac atcttctata aacttatctt
     1501 catagaaatc aatttctgga aggtatatta aatcaacaag tggagtatca ttaagtctag
     1561 ctagtgaaga agaagctgct aaccatccag tatctctacc catagtttct actataaata
     1621 taccattatt tttgtatata gaaaaatcta agtatgtttc cattattagt gtagaaataa
     1681 gctttgcagc agaaccaaat ccaggacagt tgtcagttcc taataaatca ttatctattg
     1741 ttttaggaat acctataaat tttattttac aagtagtgtt tttgcagtat tgagaaaatt
     1801 tcatcactgt gtccatagaa tcatttccgc ctatataaaa taatgtatct atttcatatt
     1861 catctagtat atttataacc ctatcataat cataagaatt tattttataa tcagccagtt
     1921 tatatctaca agaaccaaga ccagacgaag gtgtttgctt taaatttaat acattatctt
     1981 tgtttaagga gtctgaatct attatatttt tgtttaatat accttcaatc ccattgatac
     2041 caaaatatac tttatcaaag tatttagttt ctaaattttt ttcataaata ccaactgcag
     2101 aagcattgat aacagctgta gggcctccag attgggcaat aatacagttt ctcattttac
     2161 atctcccttt cattaatatt gttatgaatg gtacttttta tcgagtaaat ttatatttat
     2221 agtctgatta tatattaaat gaactagcta tatcaattga gttagtatag acaataaatg
     2281 ccataagaat gtggtaaaaa actaactttt tatatacaat taatgtctat aatttaccac
     2341 aatataaagg tacttttaag gagtagtcaa agtgttgtga aaaaaaatat atcagtgtaa
     2401 tattaattta caaagcaaaa taagttcaca gttaacaaaa gcagaataag ttcataaaag
     2461 aaatatatac agcaataaaa gtaatgctaa ataaaagtaa tactggatta aaaaatatta
     2521 aaaaaatatt aaaaaaatat caaaaactaa tatttaatac taattaaagt aaaatatgga
     2581 ggtatacaaa tatgaaacac ataaaaatag cagcaggatt agcacatgta aattacggtc
     2641 atatatcagc catagttaaa gaagcaatgg atgcaggtgt agactacgta cattcagatg
     2701 cagcagatat gcatgactta aaaaatatgc aacttatggg tggtcatcaa ataatagaag
     2761 caataagacc agtgacagat aaaccaatag aatgccatat atacacaaga gattgtgata
     2821 gattatttat agaaaaaata gcagcagcag gatgtaactt gttgataata ccagcagaac
     2881 attttttagg tgctcctctt gcatacataa taaattactg tagagaattt ggtatgaaag
     2941 taggattaac aattggatgt tacacaccat tatgttttgt tgatgaagct atatatgata
     3001 tagatagatt gcaaatagtc gttcatggtg ttgatgaaac tgatggaaaa gataactggg
     3061 gatggagaaa gagtgctgtt gatttagtaa aaagagctag aaaaatgata gacgaaaaga
     3121 atcctaagtg tgaattagca atagatggtg gactaagagc agacaatatg gagccattaa
     3181 tagaatgtaa tccagacgtt gtaatattat cttcagcaat attcaaggat aaagatggta
     3241 taacagcagg tgttaagaat tgtagaaaag ctatagatga agcagctaca aagtttggtt
     3301 tagaataaga taataaacta tgataaaaga gatgctttct taatgtttta aggaagcatt
     3361 aattttatga aaaactacag gaagctagac taatttaatt gattttttat actgaaataa
     3421 tataatcata ggaggttatt aaatgaaaaa agaaatgata aaagattatt tactgataac
     3481 aatatcagca tttttaatgg cattagcagt taattttttc tttgcagaac atacattagc
     3541 tccaggaggg ataacaggat tatcagtcgt acttagcaca ttcttatcaa tgcctgttga
     3601 gaatatatca ttaggaatat ctatgccact tttagtaatg ggattattat ttttagggaa
     3661 aggttttgga ataaaaacat tatatataac tcttatgggc ccattattct taaaagtaat
     3721 accacaaact cacataactg acaatctatt agtagcagga gtgataggtg ggttattggt
     3781 aggaactgct atagggatag caataataag gggctgtagt actggtggaa ctgatttagt
     3841 tgcaatgctt attaataagg taattaagtt tctgaagttg ccagttatat tatttatgct
     3901 agatggattt ataatagtag cgtctggaat aataagtaag aattttatga tatctatata
     3961 ttcattgata tctttattac tcataataaa aacaatagga tttgtaacta caaagtttgg
     4021 taagaatact gaagaaatta ataatataaa taatgcttgt gctcaataaa aataataaaa
     4081 tttactttaa atataaaagt ttaaaaaata taaaagctta aataaaaaga ttaaatataa
     4141 aaaatgaagg tgtctcaaaa tgaatttttt agttcatgag gcactttttc tatgaaatca
     4201 aatatatttt tatcatttgg acaatgaaaa ataggtttat ctctatgttg agacaagcct
     4261 atttttgttt atttataaga taatatttta tttttttcag aatatagttt ttttataatt
     4321 gtactaagtc tagaaatatc attcttactt aaaagagcat ttatatttat cagttcagca
     4381 ttatgttcaa agtctacagg agcagtagtt agtattaagt ccacatcatc aaagttcgta
     4441 aatgaaatag aagatagagg tatagattta actatattaa tctgattaaa ttcaaaactt
     4501 aacttttgga ctaataaatt agatacacca tcactacagt gacatattaa aatagtatta
     4561 aggggagatt tgcttctatc tattgctgat attaaatata aagcaagata tcctatttcg
     4621 tcatcattga cttcataatt catcaactgt ttgataacag tattagactc tttagcagcc
     4681 ttataagtat aaggaaatgt tctctttata tcatttaata tgggatttgt aatagtaaac
     4741 ccatagttta ttctgtgtag aactgattta aggtgattag caagagactt tattaatatt
     4801 aaatcatcct ttaagtttaa gtttagtata cttccccaat gaacagctat tgcttcagat
     4861 agattatact ctgtaatttc ttcattttta agaggttcaa gagtagtagt tttctttatt
     4921 gatttaagta atccttctat atgaagaagg atataacaga cctcttcatc aataaattct
     4981 acgtcaaaag cttcacttag gttagcacat aaaatttttg atttttcaaa gtattcatgt
     5041 tctaataatt ctctaaaaaa accttcagat aagttaatat atcttttaat tgaaattctt
     5101 ttaatacata tagctatatg tattaagaag tttatataat actcatcact aaggttactt
     5161 ttttctactt gtaaggtgtg tagagtgatt tgagataatt tctcaaaatc atagttaaat
     5221 agctccttaa atatttttgt tgaaggggta tctttgcatt cattagattt cgagaataat
     5281 atttctttaa gttcagtata acttttatta accgtagcaa gttcaaaaat tgcttttcga
     5341 atatctttct cttttccctc taaatatact ccattattgg tttttctaac tagagtcact
     5401 ttaaagtttt gcaaaaaatt ttgaataact accaagtctt tatgcacagt agctctacta
     5461 acatgtagtt cattgcaaat atcacgaatc ctaaacttag aatttttaat aaataattta
     5521 cttagaatat acctttttct atcttcagga gagtatgcat atatacaatt tgatttatca
     5581 ttgatatctc taataacttt aagttttata tcttcattac ctagaataat aacccctgaa
     5641 ccagtttttt tatctaggga taaattaaac tctaaaagcc attcatctaa aataacaagg
     5701 tcattacgaa tagttttatt actaactttt aattcttcgg caatagtatc tacagtaata
     5761 tagtctgagc tgttgagtaa taattcagca atatctaata atcttttgtt caatgcaaac
     5821 cctcctaaat cttagttact atttattaac cttggaccaa tctctttcaa aagtattggc
     5881 accatctatt gtaagctggt taaaagaagc ttttttaaat aaatcatatg ttacagctgc
     5941 ataatcacat ccacaaagag atgcttgagt taagtggaat acatttttaa tagaagctgc
     6001 aacaacttta gtattatatc catttattga tataaggtta catatttctg taaggttttt
     6061 tataccatca ttcccatttt catcatttct acctataaaa gggaataggt aatcagcacc
     6121 tgcatttata gctaatgatg cttgatttac attgaaaatc aaggtacatg cagtttttat
     6181 acctttacta tttaatatgt ttataagttc taagccaact tcagagaaat ttatttttat
     6241 tatagtgttt ttatttatat ttaatatctt atcacatgaa ttaattaatt cgtataaatt
     6301 atttccaatg acttcagcag ttaatatttc tggatttaaa ttagcacatg tctcaataaa
     6361 ttcatggaag tttactttct ctttaagata catagatgga ttagctgtta ttcccttaaa
     6421 tccatacttt aaacactttt gaatttcatc aagattagca ctgtctatca aatatttcat
     6481 aatttaattc tcctttaagc tatttgtcat aaaaaagtgt atatactcta tttttattat
     6541 taagtcaatt atacaataag attgattata aaattataac atgggtactt aataaaattt
     6601 acataatgat atggtaataa taaaacattt attgtctaat aattgccata agctttagta
     6661 aataagagtt aacagtgaag gtattgcctt aaaaatccta tgatatgtat aatttgaata
     6721 tgaaggcaat aatttaaggg aggaaaaata tgattaactt ttatcaacca tcaagaataa
     6781 attttggaca aggaacttta agtgaattac ctagaataat aggaaaatat ggaaagaaat
     6841 gtttgttagt tactactcca aatgtagagc cattagacaa gttatataaa agagttacat
     6901 tgaatttaat agaaaaaggt atagaagttg tacactttga ttaaaaaata gctacgcaca
     6961 ctcgtgactt tagtcatgag ttaatagcta aaatagtcag catataggga aacctatatg
     7021 tagagatagg atagaaccta tccaatacta cttgaattgc tggaaacccc taaagctaac
     7081 caaactaaaa cataaggatg aaataaacct aagtgtgaaa gttacgaaag tagaaaaaat
     7141 tggttagatg gtataaggtt aaatcctaag tactttaaaa tgggcaatca gcaggtaagc
     7201 tccgaatagg agaaacttca acgactattc ctcttgaggg aagtacacta caagttatcg
     7261 gtagtggaag tgggtagacc ttagcggata atgccgaagg ataagatata gtctgtgctt
     7321 atgtgaaagt gtaagaagtt cataagagaa ctgcataggt agtagcgaac ttatgtgaac
     7381 gacagcctca aaaatgataa aagttaagac ttacggttct tatatatgtt atttaaaatt
     7441 aaatataaaa ccttctaaat
//
LOCUS       DAEMIK010000173         7232 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEMIK010000173 DAEMIK010000000
VERSION     DAEMIK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.176"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            367..3009
                     /gene="adhE"
                     /locus_tag="KPK87_003362"
                     /gene_synonym="adhC"
     CDS             367..3009
                     /gene="adhE"
                     /locus_tag="KPK87_003362"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG2539476.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3370..3711)
                     /locus_tag="KPK87_003363"
     CDS             complement(3370..3711)
                     /locus_tag="KPK87_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539477.1"
                     /translation="MLLILGNLNLDLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIVNIKNQN
                     EKVILNKSIKK"
     gene            complement(3746..4414)
                     /locus_tag="KPK87_003364"
     CDS             complement(3746..4414)
                     /locus_tag="KPK87_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539478.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVAGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIEDLINLLGLKERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRVISIIDGE
                     LKLN"
     gene            complement(4426..7002)
                     /locus_tag="KPK87_003365"
     CDS             complement(4426..7002)
                     /locus_tag="KPK87_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2539479.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKMGQCEIFSNLNESKLSDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFMIIVLSMSIGGIIVIKDNYKYSFLDVQNKSVQEQTYM
                     NADFILSNVYLNETGNNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NKLDYYEAINSKPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYTFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLIYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISEREFKNMILYEGILYGVLSSIITIVSGLI
                     IQLKMYYMQGFISHGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
BASE COUNT         2517 a         1031 c         1072 g         2612 t
ORIGIN      
        1 aaattttatt tactacttat acaaataaca tgcaaataac atataaaatt taataagtaa
       61 aaaaaatgaa agatataaat tatcatttta gtattttatt tagtcgaagt aaattattca
      121 aatgaaatat atctcattta gataaaaaat tttagtaaaa gtaaaaaaaa taatatattt
      181 gttaaaaaat aaattgacaa aatattcaga aaaaaacttc ttgacctaag agagtaaaat
      241 aaataaaatc taaggtatag agatgaaatc tataaaatta ttttaagtat aagttaaaaa
      301 tatagatgat gcatttaaga tttataaaaa ttagagatgc ataattattt tagggaggtt
      361 tttagtatgg agaaaaaaga aaaggtagta gaaaaatcaa atgttgaagt atgtagtcct
      421 gaaattgtca acagtgttga aacattaaga atgagattag aagaaattag attggcacaa
      481 aaagaatttg caacatttac gcaagaacag gtagataaga ttttcttagc tgcttcaact
      541 gcagctaatc aacaaagaat acctctagca aaaatggcag tggaagagac tggtatgggt
      601 attgttgaag ataaggttat aaaaaaccat tttgcctcag aatatatcta taatgcttat
      661 aaagatacta aaacttgtgg tgtcatagaa aaagatgaag catttggttt tacaagaatt
      721 gctgagccag taggtgtttt atcagcagtt ataccaacaa ctaatcctac atcaactgca
      781 atattcaaat ccctaatagc actaaaaact agaaatggta taattttctc tcctcatcca
      841 agagctaaaa actgtactat agaagcagct agagttgtac atgatgcagc agttaaggca
      901 ggagcaccaa aaggacttat aggttgggta gatgttcctt cgattgaatt aactaatgtt
      961 gtcatggcag aggcagacct tatattagct actggtggac ctggaatggt taaatctgca
     1021 tactcttcag gtaaaccagc tgtcggagtt gggcctggaa atgtacctgc aataatagat
     1081 gagagtgcag atataaaaat ggctgtaagt tcaatcttag tatcaaaatc attcgataat
     1141 ggtatgatat gtgcttccga gcaagctgta atagtaccag aaaaaatata tgaagaagtt
     1201 aaaaaagaat ttaaatatcg tggagcacat ttcctaaata aagaagaaac tgaaaaagta
     1261 ggaaaagtag taataattga tggttcatta aatgccagaa tagttggaca accagcacat
     1321 gtaatagcca aaatggcgga tgtagaagtt ccaaaaacag ctagaataat aattggtgaa
     1381 gtagaatctg ttgaattaaa tgaacctttt gcacatgaaa aactttctcc tgtacttgca
     1441 atgtacaagt ctaaaagttt tgaagatgca gttgctaaag cagaaaaatt agttgcagat
     1501 ggaggctatg gtcatacatc ttcattatat gcagactcta taaatcatcc agagagagta
     1561 gaaaagtttg taaatgctat gaagacttgt agagtattag ttaatactcc atcatcacaa
     1621 ggtggtatag gtgatttata taactttaaa ttggcaccgt ctcttactct tggttgtggt
     1681 tcttggggtg gaaactcagt atctgaaaac gttggagtaa aacatttgct taatatcaag
     1741 acagtagctg aaaggagaga gaatatgctt tggttcagag caccagaaaa ggtttatttc
     1801 aaaaaaggat gtttaggtgt agcagcaaga gaatttaagg atgttatgga taaaaagaaa
     1861 gcatttatag taacagattc tttcctatat aataatggat atactaagaa attaactgat
     1921 ttattagatg agatgggaat taagcatact acattctttg atgtagctcc agacccaaca
     1981 ttagcttgtg caagagaagg agctaaagct atggctgatt tccaaccaga tttaataata
     2041 gctgtaggtg gaggaagtgc aatggatgct ggtaagataa tgtgggtaat gtatgagcat
     2101 ccagaagtag atttccaaga cttagctatg agatttatgg atataagaaa aagagtatat
     2161 gtatttccta agatgggtga aaaagcgtat tttgctgcta tcccaacttc agcaggaact
     2221 ggttcagaag taactccatt tgcagttata actgaccaag attcaggtgt aaagtaccca
     2281 ttagcagatt atgaattaat gccaaacatg gctataattg atgctgatat gatgatggag
     2341 atgccaccaa gattaacagc agcatcaggt gttgatgctt taacacatgc attagaagca
     2401 tatgtatcta tgttaagaac tgaaccagct gacggtctag cactacaagc aggtaaaata
     2461 atatttgaat atttacctcg tgcatataaa aatggaaaga atgataaaga agcaagagaa
     2521 aaaatggcaa tggcatctac tatggcagga atgtcttttg caaatgcctt cttaggaata
     2581 tgtcactctt tagcacataa attaggtgca ttccaccatg ttcaacacgg tgttgcaaat
     2641 gcattattaa taaatgaagt aatcaagttc aattgtgcag aagctccaaa taaaatgggt
     2701 gcattctcac aatacagata tcctgattgc atacaaagat atgctgaatt tgcttctttt
     2761 gcaggtatta aaggaagtac agaccaagaa aaagtagata atcttataaa agcaatagat
     2821 gaactaaagg caaaagtagg tcttcctaag actataaaag aggctggagt agaagaatct
     2881 aaattcttag aaagattgga tgctatggtt gaacaagctt ttgatgacca atgtacaggt
     2941 gctaatccta gatacccatt gatgagtgaa ttaaaagaga tatatctaaa agtttattat
     3001 ggtaaataat atctattgtg caacataggt tctattgggt ttgtatataa attattagat
     3061 ttgaatataa atagagtttt attagagaca cgtagaagta actataaata atagattata
     3121 atttaaattc taaatggaaa tgataaaaag tcagtaattt aatggtaatt tcgaatatgt
     3181 gtatttaatc aattaacttt aaaattagtc ggaaaatttt tttattacat ataattttaa
     3241 agcaaaatat ataaaaaact ttatgaaaaa attataaaaa tattcagaaa attctattga
     3301 ctaatttatt tatcatgata taataagttt agaaaagaaa aagaattggg gcctattaag
     3361 gcccccttac tattttttga tagatttatt taaaattact ttctcatttt gattttttat
     3421 atttactata gagtcagtat aatccacttc aaagttggca aaagcagaga ataaatctat
     3481 atctcttaac aaatgaattg tcatacattg attgttctta tttagcctta gaatcaatgc
     3541 tcttacaaat ggaaattgta ttctagaatt aaataagtta gaaccatcag tatctatatc
     3601 taagctacga taattaatat ctataaatat atctacatca tcattttctt ctcttattat
     3661 tctgtagtcg aaatccaagt ctaaatttaa attacctaaa atcaataaca tttaaatcct
     3721 cttttctata aatagtttta gagtgttagt ttaacttaag ctctccatct attatagata
     3781 taactctgtc tgcttttcta gcaatatttt cattatgtgt tattattata agggtttggt
     3841 tatatttttt tatagaactt tttagtaaat ccatgacttc ttctgtagtt ttactgtcta
     3901 aatttcctgt tggttcatct gctaggatta tacttggttt tgcagataaa gcacgtgcaa
     3961 tagcaactct ttgctgttga ccaccagata attggttggg aagatgattt ttcctttcct
     4021 taagccccaa taaatttata aggtcttcaa tatattcttt atctattttt ttattgtcta
     4081 actttagtgg taattctata ttttccttta catttaatac aggaattaag ttatactgtt
     4141 gaaagacaaa tcctactttt tgtcttctat atcttgctag ttctttatct gaaaggcaac
     4201 atatatcttt accagcaact tttatagtac cattacttgg tctctcaaga ccacctacta
     4261 aatgtagaaa tgtacttttc ccacttccac ttggcccaat tattgcaaca aattcacctt
     4321 tatttatttt taaatttgca ttctttagtg catgtacaga tgtttccttt ttcccataaa
     4381 tcttacctaa attaatagtt tccaatatag gcataattat ccctcctatt ctgtaatgtt
     4441 tatagcttca actatactaa ttttatttat ctttcttgat ggaatatatg tagctagaat
     4501 acctacaatt atatttgcta caacaactag tatatatatt ttgtaatcta ttgaaaatcc
     4561 caatccatgt gatataaagc cctgcatata atacatttta agttgtatta taagtcctga
     4621 cacgatagta attatacttg agagtacacc atataatatt ccttcataaa gtatcatatt
     4681 tttaaattct ctctcagaga taccaatagc tcttaacatt ccaaattcac tagttcttga
     4741 agttaagtta taacttatgt tgtttattat gttaagtaca ctgattatga acattattgc
     4801 tactatgcca taactataaa tcatagttct agatgtcatc ttatcagaat tttctttttc
     4861 taccatcata tctactgttg tagttccagg aacttcactt ccaattttac ctaaaaggtt
     4921 atttattttt ttcttgtttg cattttcatt aaggtctgca taaattaagt tatatttatc
     4981 tacaccagtt aattttttta ggtattcgct acttgttatt acatctacac catcatctcc
     5041 agagtacata ccattatctg caaaagtata acttataata gctccaactt taaattcata
     5101 ttctaagtat tcataattat cttttagact ttcataaagc cctgtatcca tcccaccctt
     5161 tggatatttg acttttatag tatcacctac ttttatatct acagctggtg tcccttcacc
     5221 aggagtaaaa gtctttgtgt atttctgtgt tttagaaatt tctggtacat atacaatagc
     5281 taaattttct tttttcattt ttttcaagtt tatatttcca cttactaagt atttttctaa
     5341 tgactttatc atctcatcat tataaccttt tagcttttgc tttagtgtat atttgccagt
     5401 agtcttattt tttatgaaca gaggatttag tttataataa ggttttgaat taatagcttc
     5461 gtagtaatct aatttattta ttttatttac ctctattctt gtgtttaaga tactagcagt
     5521 tttgactttg tcaattccat ctatattttt tattttatct atctgactat cactaaaccc
     5581 ttttatatct ttaaaagaag tattgttacc agtttcattg agataaacat ttgatagaat
     5641 aaaatctgca ttcatatatg tttgttcttg cacggatttg ttttgcacat ctaaaaaaga
     5701 atatttataa ttgtctttta tgacgattat acctcctata ctcatagata atacaattat
     5761 catgaacccc tttttatttc taaagatgtt tttcgcagat atagattttg ttactgacat
     5821 atttaaagtt aattttgata ttaaataatt aactttggtt acatttttat cagctccaaa
     5881 agtttttctg atagcatcaa taggagaaat tcttcttatt tttaagtatg taaagaaact
     5941 tataattagt attattagta ttgtacatgc aattgataag agaattattt tatctggaat
     6001 aacaaaaggt gtaacattta tatttccttc aaattgaata tttcctacag acctattaaa
     6061 tatttgtgca cctcccatac ctatacatat acctataggc attgctatta gagcaagtat
     6121 gaaaagttca taaagcataa gtttgaaaat cctaaagttg gtggaaccaa tagctcttaa
     6181 gacgccatat tcctgtactc tttggtatat tgatataaca tagatactat atattacaat
     6241 accagcaaat agactcatag caattgcaac gtatttactt gaattatcta aagtaccgtt
     6301 tttcatagta gattctataa gcattgagtt tcttcctata cttttctcat ctaacatagc
     6361 attttttatt atattgtcta tgtttgtatt tatatcactg ccttcattaa attcaatata
     6421 agttgtaaaa tcacttagtt ttgattcatt gagattagaa aaaatttcac attgtcccat
     6481 ttttttctct ataaatctat cttcaaggat tccaacaact ttaaaagttt ctggattttc
     6541 tttttgatat aatttaaaag taaggttttg atttatctct ggttttaagc ccatactatt
     6601 taaaatccat ttctcgacta ccacctcatt gctagctttt ggaaatcgac cttttataag
     6661 tttagaatga ctagtcaaat aatttttatt tgcatatagt atgtttatag gtaatttttc
     6721 attaacatct gtagacgcat agtaagatgt tatacccaga tttttaatat ctttgctaga
     6781 ttttatggac tttaattgat ttttctttat atttttaaag tatacatggt cagttccaag
     6841 ctctcttttc aatctatcta catctgcttg ctgagcactt cttgagaggg taccaatacc
     6901 aacaacgagt gatgtagcaa gaattatact cacaacaatt gctaaagacc tagctttata
     6961 actttttata taccttagta ctattttcaa attatctttc atatttacca cttcctttct
     7021 taactaaatc aattatatat ttatctaaaa tatatagcaa agtaagtatt ttcaagtttt
     7081 aagtagttaa ttgtacaaat tcatctgtat ttttacaagt ttaattaatc tatatgtaaa
     7141 ttttaacttt ttacataatt tttttaaaat gatataatcc ctatataaat tgtagagggg
     7201 aggggaatca tggataacaa ttttgtgttt cc
//
LOCUS       DAEMIK010000174         7154 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEMIK010000174 DAEMIK010000000
VERSION     DAEMIK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.177"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            426..1118
                     /locus_tag="KPK87_003366"
     CDS             426..1118
                     /locus_tag="KPK87_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539480.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1314..3374
                     /locus_tag="KPK87_003367"
     CDS             1314..3374
                     /locus_tag="KPK87_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2539481.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDIAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3579..3902)
                     /locus_tag="KPK87_003368"
     CDS             complement(3579..3902)
                     /locus_tag="KPK87_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG2539482.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3895..4596)
                     /locus_tag="KPK87_003369"
     CDS             complement(3895..4596)
                     /locus_tag="KPK87_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG2539483.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4874..6259)
                     /locus_tag="KPK87_003370"
     CDS             complement(4874..6259)
                     /locus_tag="KPK87_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2539484.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6367..>7154)
                     /locus_tag="KPK87_003371"
     CDS             complement(6367..>7154)
                     /locus_tag="KPK87_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2539485.1"
                     /translation="SPAIIGLTRCKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKL
                     LISTICIIYWFNPILYLLKKYFHELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQK
                     TLKYSSFVSQFLNKKNIILKKRLDSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFK
                     QNEDVEYTIGKINYNKNGETYGTHIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDC
                     DNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT         2752 a          817 c         1026 g         2559 t
ORIGIN      
        1 aagtagctat tattgcagtg aaatgaatgt ttctaagtat atttgggtgg aagaatggtg
       61 gaagaaaatt agtgaaatgt aattcttcca ccattcttcc acctagaaaa tagaaactgt
      121 ttaataagaa ttaatataca ttaattcagc tgaatgcaat caaaatactt aaatgaaagt
      181 aatctgaaaa caacataatt caaatcaatt tattcaaata aacctcaatt tatcttaaat
      241 cttaagaaat atttaagaat taggataata ttttcttaag atttatattt tattatatag
      301 ttataaggga aagttacaaa aatgtaagcc aaagtaatat aacaatttag tcattgttga
      361 gaaagggagt gaaagatatt gtataatata gataagtatt attatacaat agaggaagtg
      421 aaaatatgta caatatatta gtagtagacg atgataaaga aattgttgat tcgatagaaa
      481 tatatcttaa atcagaagga tttaaaatat ataaagcata tgatggaatg aaagctttgg
      541 aaataataac agagaatgac attcatctaa tactaatgga tataatgatg cctaaattag
      601 atggaatcaa agcaactgta aaaatacgag aagaaagaaa tattccaata attttaattt
      661 cagcaaaaag tgaagataca gataaaataa taggattaaa tattggagct gatgattata
      721 taacaaagcc atttaatcta ttagagctta tagctagggt taaatcaaac ttaagacgat
      781 atgtaacttt aggtaattat gaagatacat gtaaagaaat tctaaaaagt ggagctttgg
      841 agttaaatac actaacaaaa gaagttaaaa ttgatggtga aaacataaaa atgactccaa
      901 ttgagtataa aataattgaa cttcttttag aaaataaagg acgagttttc tcaatagatg
      961 agatatacga aaaagtatgg aatgaggaaa gttttaattc agaaaataca gtagcagtac
     1021 atatcagacg aataagagaa aagattgaaa taaatccaaa agaaccaaga tttttaaagg
     1081 tggtgtgggg agttggttat aagattgaaa aattttagtg gaaaaattat aaaagaaaag
     1141 agtttaatat ttaattgcat tttgatttta gtcattttta catttacaca aatgttagga
     1201 attttaatat gttttaattt tttctctgag agtttaattt actctatggg gattatgctt
     1261 ttcttaaact ttattttata taaaatactg acaatgtaga tgaggtgggc aacttggaga
     1321 aatatagagt attaaagtta gtttcattaa tcataatatc tatctcattt ccattaacta
     1381 tattgttggt gacaatagga cttgctgagc ctaaatacaa tatacaaaaa acttctacaa
     1441 agagtgggat ttttgatata tctgatgatt tttatgatag taatatattt gacgaaggaa
     1501 caatggcaag tctattaccc aagtttaagc tatctgtagg tggtaaatta actagtaaag
     1561 aaaaaggtaa attccaacaa gaaggtaaaa gtatagattt aagtagtagt gattctgagt
     1621 atgataaaat catgattaat gaacaagctt ctgaagaata tttaagttat ctaaaaaata
     1681 taaaagtgta tgtcattaat acatacacag gagtatttta tacaaatacg tcatatgcta
     1741 gtttatatga ttttaaacaa aaaactaaga gctattgtaa tgtagagata ataaataaga
     1801 taggaaagag ctcatatatt aaaacaataa atggagaaaa atttgaactg aataatctga
     1861 gtctggaact aaatgaagta gatgaaggtt ttgaggcata tgtatctttt ccagaagagc
     1921 caactataga agatggaata gtatatacta attttcaaat atttaaacaa gctacagaaa
     1981 aagtaagatt attttttgta gctagtttaa gtttagcaat tatatgttta ttatctataa
     2041 ttttatatat caaaataaaa ggtgaagttt taaaaagaaa taatgtattt ttactaatat
     2101 acagtaagat acctatggaa tttaatgtaa tattagtatc tatttcaatt gcaattttaa
     2161 ctatatatgc tcttgctgta tcttatcata ttgatgtaat tgcattagtt tctttatctt
     2221 tactgatagt atcaaataat atatttttta taaatgtgca attgagttat ttagaaaata
     2281 aaatgtgtat atttaagaca agtattatag taagagtgat tttatggtta ataaaaactg
     2341 taagggaggt cagtaacgct ataaagaaag tatctttagc aaggaaagtt attgttatag
     2401 tgataatatc tttagcttta atcatggtaa ttagcactcg aagtataggt atgggattac
     2461 tgttttctat atgtattttt ttaggattta caatatatat aactaaaaga ctttcatatc
     2521 ttagttacgt aatagaaggt acagaaagaa taaaaaaggg agaattagat tataagataa
     2581 aaattactgg gaatgataat tttacgtctt tagctgaaaa tataaataat attggagaag
     2641 gtcttgatat agctatagaa gagcaagtaa aaagtgaaag aatgaagtct gaccttataa
     2701 caaatgtatc tcatgatttg aaaactccac ttacttctat aataaattat gtagatttaa
     2761 ttaaaaaaga agaatcaata caaccagagt atataaatga ttatataaat gtacttgagt
     2821 caaagtcaaa aagactaaaa ttattgatag aagatttatt tgaagcgagt agagtaagta
     2881 gtggcaatat agaattacaa attagcaaga ttgatttagt gcaactatta agacaaagta
     2941 taggtgaact tgaagaaaaa ttatctaaaa ataatttata tttaaaatta aatgttccaa
     3001 atgataaggt gtatatatgg gcagatggaa gaagaatgta tagagtattt gaaaatcttt
     3061 tatctaatat agcaaaatat tccttagaag aaactagagt ctatattgat gtatatgact
     3121 atggtgaaaa tgtaaaggta actatgaaaa atatatcttc ttatgaatta aattttgacc
     3181 caagtgagat tatggaaaga tttaaaagag ctgatgaatc aaggaatact gaaggaagtg
     3241 gattagggct tgctattgca agagatttag tagctttaca aggtggaaaa ttttatatag
     3301 aaatagatgg agatttattt aaagcaaatt tagagtttca aaaatatgaa gaagaaataa
     3361 caaatatgga atgagatagt atttaagtat atatattttt ttactgtcga aagcttttat
     3421 tataatatat tagaatattt acagtagaga atgaagagta aaaacagagt aactttaagt
     3481 cactctgttt tttatttaaa atcaataata taacaactta aattaaaatt gtacacatag
     3541 ttttaaaata acttatacaa taaaacatta tccaaatatc acaagataat tccaaataaa
     3601 tatatagata tcactcctgt aataatagaa ataccaatag atttaaactt atatgcaaca
     3661 agaagtacaa tcataaaaga tataatttta gggttagcta tagataaata tagactatct
     3721 ccattaaaaa atacatcttt agcagttaag gaagtaagta ttgctacagg tataaatttc
     3781 atgatttttt tcaaaaaatc aggcatctct ttttttgaaa aaattaacat aggaagcact
     3841 cttggtagat atgtaacaat gtacattcca attataataa aaaatatata attattattc
     3901 ataagattcc tcctttttac agtaagattc ttctataaag aaaccaatta aagatgctaa
     3961 aattgatgca actattatta ctaaattgct tttgaataat gcatataaaa taacagaaat
     4021 aatgattgat gatataaata ctacaatata aatcttattt tttatttgag aaactattaa
     4081 agctataaac atagaagtca atatgaagcc agaaatagaa ctgtctatag atatactttc
     4141 tcctaaaaat gctccaataa aattagctaa aactgatgaa attaagcaga agaaattaag
     4201 ataaatagca tttctatcag accaatttcc agcagtaaat ttttcataat taactgcaaa
     4261 agtttcatct gttatagtat gacaaaatgg caatataaat tttggacttt tattttttat
     4321 gtacatgttc aaattagaac tcattagaaa atgtcttaag ttaactataa atgtagtcaa
     4381 aacaatagat gttacacttg caccagtaga aatcattgaa gctgcaatga attgagaact
     4441 tcctccaaat actaaagtta ccattagtgc tatttgaaaa ggtgaaagac cagatttttg
     4501 taatagtatt cctcctacta tacctatagg tatataacct aagaatatag gaattgaagc
     4561 aataaagctt tcttttaatt gttgagttct ttccacaata aatcactcct tttgtattaa
     4621 atgtattaaa gtattcgtaa aacagaaaaa tagattaatt gtataattgc aattataatt
     4681 aaaaataaat ttggattaat atataaaata ctatacaaca aatagataat cttaaagggt
     4741 tattaaattt tatctatttg ttattatgat gtaataatac atattaatct ctataagtta
     4801 attactatct ggtgtaattt tattttaaca acaaaaaaaa atacacacaa ttaaaatatt
     4861 gtgtgcaata caattatttt ttctcttcta atagtctttt taatacgctc aaaccagctt
     4921 taagttcttc tgtactttct gtagcacata cagctattct tattgcagaa attggttttt
     4981 cttttccaac agcaaaacgt tcagaagcat agacctgtac accttcttta agtgcttgta
     5041 tctcaaattt ttctgccatt attccatcag gtagaatcaa ccatctaaat atacattctt
     5101 catttcccaa aagattatat ccagataaat attgattaat aatcttgttt cttcttcttg
     5161 cagatttacg gcgtgcatta attaatttat cggcttcacc tgatgtaatc atgcgataag
     5221 caatttctgt taaaaaataa gattgagata gattaatgtt gtataatgcg cttgataatg
     5281 cctctcgata ctgtttaggt gtagagatat atgctagtcg taaactaggt gcaataattt
     5341 ttgataaact cgtaatataa attgtttgat tgggcagata ggaagccact ggatttaaat
     5401 gtgtttcatt aagtaaacta tgaatagcat cctcaataac aattaaattg tatttagatg
     5461 caatatttgc aatcattttt cgtccatttt gtgacattat atgtgtagtt ggattttgat
     5521 aatctggtat tatgtataga cctttaatat tttcattttt acaagcatat aatagtcctt
     5581 cttcactaat ctcattatct tcttgtttaa ttggaattag ttgaatccca agcattttag
     5641 ctgctgtctt tattccagga taagttaatg ggtctacacc tattctatct ccatgttgaa
     5701 acaaaccagc taaaatagca acaattgcat tttgtccacc acttgcagga agtaagctat
     5761 ctgatgtagt ttcaagccca gctttataaa ttaatttggc aatagcttct ttttgccatt
     5821 tagcaccatc tctttgtcca tattggaata tggttttaaa atctatttcc ttaattatgt
     5881 gtttaaacaa agtattcatt tcttctagcg tgaaatctgg attcattgtg cccatttcaa
     5941 taaatgtaat tttattatta ctggacatca agaatagatt tgaacgtgta tcataagata
     6001 caaaagttcc acttcctgta actccactta ttagaccttt tttttcacaa attttaaaag
     6061 ctctagagat agtactcaca ttaacatcta agaaatctgc caattcacgt tggggtggta
     6121 atttagtacc tggtaataaa aatccattgt tgatatcttg ttctagttgt tctgcaagag
     6181 ccttatataa tatttgccct tttgaaggtc tctttggttt ccagttcata ggataatttt
     6241 caaaagaatt aattggcata agttttcacc tcacattatt aatttgattt tttctttatc
     6301 taaaacaata aattctaaag atacaaataa aaactgatag gaaataattc tatcagtttt
     6361 tgtcttttaa ttgatttcaa atcttcctat aatttttata ccttctttat cataaagagg
     6421 gattgttttt ttaaatatat taaaatttct ttttcttgta tataaaacag cttcttctgg
     6481 tgtctttggt tgattatcac aatcgtacaa atctcttttt tttacgtagc cgactttttt
     6541 acctctacct attgtttgta taagttctgg ttcaatggaa tttccatttg agtctattat
     6601 atgtgtacca taggtttctc catttttatt ataattaatc ttaccaatag tatattctac
     6661 atcttcattt tgtttaaaat tggagttgat agaaaatatt gaccctatta ttataaaggt
     6721 taataaaaac ttaagaccta aattaatttt ctttttatct tgattaaaca tagaatctaa
     6781 cctctttttt aaaataatat tttttttatt tagaaactga gatacgaatg atgaatactt
     6841 caaagttttt tgatacttta ttgtatctaa tattatatat gcatactctt taatttcact
     6901 cgtggtacac ttgtcaatca caatttcatc acaagataat tcacataatt catgaaaata
     6961 tttttttaat aagtataaaa ttggattgaa ccaatatatg atacaaattg tactaattag
     7021 aagcttaaat aagttatctt ttcttttata atgaattaat tcatgtttaa agatgaatct
     7081 taactcttta ttattgtact ttttttctgg aattattatt ttacagcgtg ttaaacctat
     7141 aatggcagga gaat
//
LOCUS       DAEMIK010000175         7126 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEMIK010000175 DAEMIK010000000
VERSION     DAEMIK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.178"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            181..831
                     /locus_tag="KPK87_003372"
     CDS             181..831
                     /locus_tag="KPK87_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2539486.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1126..3192
                     /locus_tag="KPK87_003373"
     CDS             1126..3192
                     /locus_tag="KPK87_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG2539487.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPNG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKDAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLVIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQRLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3209..5164
                     /locus_tag="KPK87_003374"
     CDS             3209..5164
                     /locus_tag="KPK87_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG2539488.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDISSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5180..6370
                     /locus_tag="KPK87_003375"
     CDS             5180..6370
                     /locus_tag="KPK87_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG2539489.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
BASE COUNT         2645 a          836 c         1357 g         2288 t
ORIGIN      
        1 tgatttgcaa tacaatgaat taatctatag tataagagca tgatttacag tacaataatt
       61 taaattgtag tacaaaagta ctatcaaata agaatttcta ctttaaaaat attactaaaa
      121 gtaattaaag tccagtaatt caatataata tggcaaaaga atagaaataa ggtgagagta
      181 gtgaaagtaa agtcaaattt agatgaaatt ttatatcata agattattga aagtcttatt
      241 cgtggtgaat actctgtagg tcaaaaaata ttactaaatg acctttgtga aaaatttgaa
      301 gtaagcagaa caccagttgt acaagcagta aaaatgctaa ataaagatgg agtgcttact
      361 attatgacaa atggtaaagt gtacgtacca gagtatgaat atgatatggt aaaacaagtt
      421 tgtgaaacga gaacactgat tgaaacatat gctttagaaa aaatgatgca agaagaggaa
      481 gaggtttttc aacaaaagct agatgtaata aaaagatact ctgataaatg cgaggagtac
      541 tatcgtcaag aaaaatctgt agaacttgca ctagcagatt tggaattaca taaagccatt
      601 gtagaagggg caaataatga gattttaaat gatgtatatg ttggtataca aggaagattt
      661 atagtagtga actatttaat tcgaccatta aaaaatcgta attatgaagg aacagtacaa
      721 gaccattttg aaatattaaa attcattgaa gacagagata caaaaaaagc agtagataaa
      781 ctaagaaatc atattcaagg aactattaaa agattctgtg aagatgaata ataagttttg
      841 aagttactat atatacttat ctattaagtt gctaaatgta gtctaagtat aatctaatta
      901 tagtttaaac ataatctaaa tataatctaa taaatataat ctaaatatag tgtaaataag
      961 tctaagtatg aattaaatat agattaagta tagattaagt atgaattaag catgaattaa
     1021 gcatggatta aatatacttt aaattacaaa tattacaagg aagccagaag gcttcctaaa
     1081 aaaataacct ttataaaatt ttttatttta aatttaaaat aaaaaatgaa agaggtggta
     1141 gagagtttga aaaaaattac taaagaaagg atagaaaata ctatgaaaaa gctatttacg
     1201 agtgaaacat ttttggaata tcaatttata agtgattgcc aaatatcacc aaatggagga
     1261 tatacagcat ttattgtaaa aaaagctgat atcaaagaga atggttacac aagccaagtt
     1321 tatatattaa acaataaaac tggcgaatta aaacaaataa cgtctataaa ctctgttgga
     1381 gcatatgcct gggaagatga aaatactatc ttgttccctg ctttgcgaaa tgaaaaagta
     1441 aaagatgctg tcaaaaacgg taagcaatgc atgagttact atgctttatc tttaaatggt
     1501 ggagaagcag aagaactttt tagactccct atcaaaggtg gtaaattgaa tcctatagga
     1561 aaaggtttgt atgcagttat tgattcttat gacaatgacc gacctattgt agagggattg
     1621 ccagaagaag aacaacagaa aaaaataaat gcttataaca aaagacatta tgaacatttc
     1681 aaagaaattc cttatgctgt caatggggag ggatatatta gcagaaaaag aaaacgccta
     1741 tatgtatacg atagtaatat aaatgagttg aaggcaatta cagcacctat gtttaatgtt
     1801 gtaggtatga aaataagtga tggaaaaatc ttatatatag ggcaagagtt taaagatgta
     1861 aaaggtttga aaaatggggt ctatgtattt gatacaaaca caaacacaaa tgtctgtata
     1921 ttagaaaaag ataagtatat tataaaaggt tttgaattat atcaaaatca agtgatacta
     1981 aacctaactg atgctttatc ttatggaaat ggagaaaatg gagattttta tactattgat
     2041 attgatacga aagaaatgaa acttctttca gggcatcaac atcattgtat tggaaataca
     2101 gtaacatctg atgttaaaat gggtgctggt cagacaacaa aggtagatgg agaatatatt
     2161 tattacactt ctacagtaga tatggactgc attattgaaa gaatacatat tccaacagga
     2221 aaacaggaaa aagtaacaca gacaggttca gttgatttta tagatgtaaa agatggcaat
     2281 attgtatgtg tgggttgttt aggaaatgga ttaccagaag tttatacagt agagaatgga
     2341 acattgtgta agaaaacaca tttaaatgac catattttag aggaatataa aatttctgtg
     2401 ccagaataca tagagtcaaa aggaagttct aaatgggaaa tacaaggata cgtatataaa
     2461 ccagtagatt atgaggtggg gaaaaaatat ccagctgtat tggtgattca tggaggacca
     2521 agattgacat atggaccata ttttatgcat gagatacagg tatttacaag tgcaggttac
     2581 tttgtattct tctgtaaccc aagggggtct gaaggtcgtg gcaatgcttt tgcagatatt
     2641 aggaaacagt ttggagatat tgattatata gattttatgg aatttacaga tactgtactt
     2701 gaaaaatata cagacattga taaaacaaaa ttggctgttg aaggtggaag ttatggtgga
     2761 tttatgacga attggataat tggtcataca aaccgttttg ctgttgcttg tgctcaacgt
     2821 tctattgcaa actggagtgg catggagggt acaacagaca ttggttatta tttctgcaaa
     2881 ggacaaacag gtgcttctca tatggaaaac cacgagcttc aatggaaaca gtcacctctt
     2941 gcttatgctg ataaatgtgt tacaccgact ttatttttac atggtgaaaa agactataga
     3001 tgctatatgc aagaagcatt ccagatgttt tcagcattga aaattcatgg gtgtcctaca
     3061 aaactttgtc tatttgaagg agaaaaccat gaactgtctc gttctggtag accaaaacaa
     3121 aggctacaaa gattagttga aatgctagat tggttttcgg tatatataaa aaaagataac
     3181 ataggacagt agaaaggaga atataaaaat ggaaaaaata aagattgaaa gttcagatat
     3241 ttttgaatat atattccctc atgatatttc ttgttcacct gatggaaagc atattgctta
     3301 tattatatca aatatcaacg aggaaaaaga ctgctatgaa catgacttat atgtgatgga
     3361 catcaaaaca gaaaaacaaa tacatatgac tcaaacaaaa gatgtaacat catttagttg
     3421 gatttctaat acagaactac tttttacatc taaaagaaat aagccaaaat caggtacaac
     3481 agatttttac actatttcta tagaaggagg agaagcaaaa aaagcatttt ctattccaaa
     3541 agcttgtagc gtacctgtat cacttggaaa taaattgtgg ttactgacaa caaaaaatcc
     3601 aacggattct aaaaaatcag aaccagatag ggcagtagag ggtgtagatt attggacatt
     3661 tacagataaa ccttttataa gagatggaga aaatttttct caaagaagac gtgtaacatt
     3721 agaattatat caagaaggtg aaaatataac aaaagccatt acaccaaaat tttgtgaagt
     3781 agcaggtata gacatttctt ctgataaaaa cagaatttta tatactggtc agatttatga
     3841 agattgtgca actccttttt ctggactttg ggagtatcat atagacagtg gtgaaaccaa
     3901 agaacttgta ccacaaggaa agtaccaaat aagtttggca aaatatattg gcaaagataa
     3961 agtaatgttg caagcttcaa cattagaccg ttctattaca cagaatcatg atatttttat
     4021 acttgatttg agtactagtg aaataaatat gattgctagt ccagatggta tgtatgctac
     4081 attactggat gtagatgcag tatatggtgg tggacgtagt aataaagtta taggagacaa
     4141 atttattggt gcaagaattt gcagaactat gacagaattt aatgagtttg atacaaagac
     4201 aggtaatata cgtatcatca caaaagtaga tgcttttaca tcttttgata tttacgacaa
     4261 caccatgtac acagtaatgt tgaaagatta tgagttagca gaaatatata gcattgatat
     4321 gactacaggt acaatgaaaa aaatgactgc tttttctaaa ccatatttag atactcataa
     4381 ggtttcatta cctgaaaaat tgacgtttgt agcaaaaaat aaagaagaag ttgatggttt
     4441 tgttataccg cctatagatg ctaaagaagg cgaaaaatat cctgctgtat tgttcattca
     4501 tggtgggcct aaatgggcat atggctatat gtttacacac ttgaagcagt gtgtaacatc
     4561 aaagggaatg tatgtgattt attgtaaccc tcatggtggt gacggttatg gtgaaaagtt
     4621 cttagaaatg gtagaaagat gggggtatgt tgactatgag catcttatgg aatttgtaga
     4681 tacttgtatt gaaaaatatc ctgggattga tgcagaccgc ttaggggttg cagggggaag
     4741 ttacggtggt tatatgacaa actggataat tggtcataca gaccgtttca aagcagcagc
     4801 ttctcaaaga ggtattagta acttgattac agcttcttta attattgact ttggagatag
     4861 aattatgaag cagacttgtg gagataagac gccttggaat catgaggagg ttctttggaa
     4921 tcattcacct attaaatatg tgaaaaatgt taagacacca acattgttct tacatagcga
     4981 tagggattat agatgtttta tgggggatac tttccaaatg tttacagcat taaaacagct
     5041 tggtgtagat acagaaatgt atttgtttca tggagataca catggattgt ctagaaatgg
     5101 aagaccatct aatagaattg caagagcaaa tgctattgta gattggtttg aaagatattt
     5161 ataataaggg ggttattcta tgagtgatat gttattatgg tttagtgtcg tattagtttt
     5221 atttgctatt ggggatttaa ttgcaagcaa aacaaaagca aaagtatcag ctgtatttgt
     5281 tacactactt ttatttttga ttctatttgt cacaaaagca ataccagctg atattattga
     5341 aaaagcaggt atgaccgcag cagctagttg gagtgttcca atgattatgt ttagtatggg
     5401 aacaatgttg aatgttaaac aatttataga cgaatggaga acagtattga cagcatggct
     5461 tggaattgta gcagttatag tctgtgtctc attatgtatt cctttgtttg gaaaatcaac
     5521 agtattgaca agcataccag ttataaatgg agctttacct gctactacta ttatgacaca
     5581 agcagcacta gaaaaaggac tgacacttgc agcagctaca gcaacagttg tatttgcaat
     5641 tcagaagttt attggtacac caatagcttc aagagcagcg ttacaagagg caaaccgtct
     5701 tttagtagaa tatcatgaag caaaatcaaa aggcattgat ttagcaaatg ttgatactga
     5761 taagaaagaa gcagagaata caggcacaaa agtaaaacaa gctttctgtg aaaaatacga
     5821 taaatattat tctacaaatg tttgtatatt ttttatagca ctattctctt atctagggta
     5881 tgaactttca gagattattc atgtaaatta ttctattgta tgtcttgttg ttggtgttat
     5941 tgtaacacgt attggtattg tgccaaaaga cattttggaa aaaggaaaaa tcaagggatt
     6001 tatcaacatg gttgtgtttg cagctgttat tccttcttta gcaaaagttt ctttgacaga
     6061 tttaattagt ctttttgtac caattgtggg tatgtttgca gcttctatca ttggtatatt
     6121 ccttatgatg aaggtgttac caggttggaa aattatcggt tctaaacctc ttgcttttgg
     6181 agtagggttt tgtcagatgt tgggattccc aactacatat ttaatttcaa atgaagtgtg
     6241 taatgctgtt ggagaaacgg aagaagaaag agcatatttg atgtctaaga ttatgcctaa
     6301 gctggtagtt ggtggtatgg cttgtatgat ttctattgtg gtagctggtc ttatggtacc
     6361 aatgttgtag ataggtttca aagttaaaaa agtggtatta atttatagga gaagctaaaa
     6421 agtagtatta atttataaaa taattttata ttagttaatt tgttataaga atattttctt
     6481 tttataatta taattttgat tttataagag agcagatttt ttatttgcta tgctaatagt
     6541 tataatttgt ttttatttgc taaaatgcaa ctttaatttg atttatgtat aatatagggt
     6601 atttaataat atttttgaag aatttttata aaaaattaaa aagaggagaa aaaagggagt
     6661 ggcacaaagt gaacttttta gttcataagg cactcttttt tgtacgaaat cgaatatatt
     6721 ttcaacgttt taaaagtgag aaagagactt atctctagtt tgagatagcc aattttaatt
     6781 attaaaatta ttttttgttt tatttatcaa agagtatccg tgttttagaa gttagtattt
     6841 tttattttat atatccaagg ttttccgttt ctaatgtttt cttttttaaa ataattaggg
     6901 tattcaatta tggtaaaagg tggatggtat tttgcagtga tattatttaa agcacagtat
     6961 tgaatgttga aatcctcaca ttttagagta ttatctttta taatcaaaaa aatcttctat
     7021 acttatagca aggttatttc tgcaaatatt ccatgcatca taatatctaa atttaaattt
     7081 ttttaatata gagtttacct ctagtctaca ctcttgaata actctt
//
LOCUS       DAEMIK010000176         7068 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEMIK010000176 DAEMIK010000000
VERSION     DAEMIK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.179"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            68..141
                     /locus_tag="KPK87_003376"
     tRNA            68..141
                     /locus_tag="KPK87_003376"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="KPK87_003377"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="KPK87_003377"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2558
                     /locus_tag="KPK87_003378"
     CDS             540..2558
                     /locus_tag="KPK87_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2539490.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3086..5098
                     /locus_tag="KPK87_003379"
     CDS             3086..5098
                     /locus_tag="KPK87_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2539491.1"
                     /translation="MRRNTKLLTTGILSMAIVAPAMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDTDSITLNYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINGKNNPKTLNRQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5340..>7068
                     /locus_tag="KPK87_003380"
     CDS             5340..>7068
                     /locus_tag="KPK87_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539492.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     SKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKE
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDDIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIY"
BASE COUNT         2944 a          789 c         1169 g         2166 t
ORIGIN      
        1 gatatatatg tagtttttag agtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagcgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattgtta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttatg tacttattta taattttggg taaaaacact ttgatataat
      481 ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat gttaaataag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag ggattctttc aatggcaatc gtcgcaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatcgtt ttaggtagta cctcaaaagt cagtgttaag tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgatatgcca ttggatacaa
      781 ctttaaacta caatcaatca actgagtcat atgaaggaac tatcaattat aataaagacc
      841 cagaatatct aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa caaacaagaa ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cacaggaatg tataattgaa gatataactt ctagaaaaga tgtaaataaa tatttaagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atacgaaaca gcagttaaaa
     1081 taagtaaaga aggctggaaa aatggttcag ataaggtagt tataataaat ggggatgtaa
     1141 gtatagatgg cattatatca actccactag caactacata taatgctcca atacttttgg
     1201 ttgaaaaaaa caatgtacct aatagtgtaa aatcagaatt aaagcgttta aatcctagag
     1261 atgtaattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtctaa agggctctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtatacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa
     1501 ttatattaac tgataaaaat agtattacag acaatacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggcc ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatga tataactaaa gataatgtta ctaataatag agtatatgga gcagatagac
     1681 acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagtta gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg gctgcgaact
     1801 taaaatctcc aatacttata acaccaaaga catatgtatc tgcataccat aaagaaaact
     1861 tagaagctaa atcggcaaat aaggtatata aaataggtgg aggattgact tctaaggtaa
     1921 tgagctctat agcatcatca ttatctaaac acaatacgac tccaacagaa ccaggaaata
     1981 gtgggggcaa gacagttatg attgacccag gacatggtgg ttcggacact ggaacaacag
     2041 gtaaaccatt agggggtatt agagaaaaag actatacatt aaatacttca cttgcgacaa
     2101 ctgaatattt acgttcaaag ggattcaatg taataatgac aagagacaca gataagactt
     2161 tatctcttgg aaatagaact gctttatcta actctttgag accagattta tttacgagta
     2221 tacactataa tgcatctgat acaactggga atggtgtaga agtattttat aagcttaaag
     2281 ataaagatgg agggactact aaaactgtag ctacaaatat attaaataga atcttagaga
     2341 aatttaatct aaaaaataga ggtgcaaaaa caagaacact atcaactgac cctacaaaag
     2401 attatttata tgttttaaga aataatgata tgccagctgt acttgtagaa tgtgcatttt
     2461 tggataatga gaaagatatg agtttactta atacatcaaa taaagtaaaa gaaatgggta
     2521 cacaaatagg taaaggcata gaagattcat taaaatagtc tacaatgaga gtatagaaaa
     2581 atttaaaata aattaatctg caattttaaa tttaaactaa agctataaga taagaatgta
     2641 attataaatg taattataat agtaataaag actgtatata cattgtgcat ataaatgcga
     2701 aatgaggtat atacagtctt tttataatta attgtaaaat gatttatgtt atatgaaaaa
     2761 tttacatata ttaaggctac agttaattgt caagagctgg agtattcatt tgtaatacac
     2821 aatataagct taattactat attttattgt gtgtttgtgt gttttttgtg gatttttatg
     2881 tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt tttttatttt
     2941 ttgagtttta taagtttatt tttatatatt acttatatat tacttatata ttatttatat
     3001 tttgggttag atacactttg acatattttc aaaagttaaa agattaaaaa taaagtagaa
     3061 atttatgtta agtaaggaga ttaatatgag aagaaataca aaattattaa caacagggat
     3121 tctttcaatg gcaatcgtcg cacctgcaat ggcatttgct actgaatcta atgctatgga
     3181 aaataacgct gatttaaata taaacttaga gaaaaaaagt atcgttttag gtagcaaatc
     3241 aaaagttagt gtcaaattta aagaaaaacc agatacagat agcattacat taaattataa
     3301 atgctatgac atgccattgg atacaactct aaattacaat caatcaactg gggcatatga
     3361 aggaactatc aattataacc aagacccaga atatctaaat gtttgggaac tacaagggat
     3421 aacaataaac ggcaaaaata atcctaaaac tttaaacaga caagacctag aaaagatggg
     3481 attaaattta aaagactata atgtaacaca ggaatgtata attgaagaca taacttctag
     3541 aaaagatgta aataaatatt tgagaaaaac ttcttcacct attacagaac ttacaggaag
     3601 tgatagatat gaaacagcag ttaaaataag taaagagggc tggaaaaatg gttcagataa
     3661 agtagttata ataaatggag atgtaagtat agatggcatt atatcaactc cactggcaac
     3721 cacatataat gcaccaatac ttttggttga aaaaaacaat gtgcctaata gtgtaaaatc
     3781 agaattaaag cgcctaaacc ctaaagatat aattataatt ggagatgaga atgctatttc
     3841 taaaactact gctaatcaaa ttaaatcaac tgtaaatgct agtcaaacac gtttaaatgg
     3901 ttctaataga tatgagacat ctttattgat agcaaaggaa atagataaaa atcatgatgt
     3961 ggaaaaagta tacataacaa atgctaatgg cggagaagtg gatgcactta ctatagcagc
     4021 aaaagcaggt caagacaagc aaccaattat attaactgat aaagatagta ttacagacaa
     4081 tacatataaa tggttaaaga gtgaggattt acaaaatgct tattttatag gtggtcctca
     4141 aatgatatca acaaatgtta taaataaggt aaatggaata actaaagata gtgttactaa
     4201 taatagagta tacggagcag atagacacga aacaaatgca aacgtaataa aaaaattcta
     4261 tacagatgat gagttagagg ctgttttagt agctaaatca gatgtacttg ttgatgcttt
     4321 agcagcaggt ccattggctg cgaacttaaa atctccaata cttataacac caaagacgta
     4381 tgtatctgca taccataaag ataatttaga agctaaatca gctaataagg tatacaaaat
     4441 aggaggagga ttgacttcta aggtaatgag ctctatagca tcatcattat ctaaacacaa
     4501 tacgactcca acagaaccag gaaatagtgg gggcaagaca gttatgattg acccagggca
     4561 tggtggttca gcacctggaa attcatctgg aggaatgatt gaaaaagatt acaatttaaa
     4621 tacttcactt gcaacaactg aatatttacg ttcaaaggga ttcaatgtaa taatgacaag
     4681 agacacagat aagactttat ctcttggaaa tagaactgct ctatctaatt cattgaaacc
     4741 agatttattt acaagtatac attataatgg ctcaactaat aaacaaggtc atggtgtaga
     4801 agtattttat aagcttaaag ataaaaatgg agggactact aaaactgtag ctaccaatat
     4861 attaaataga attttagaga aatttaaact tacaaataga ggtataaaaa caagagtact
     4921 tcctagtgat tctacaaaag attatttata cgttttaaga agtaatgata tgccagctgt
     4981 acttgtagaa tgtgcatttt tggataatga aaatgatatg agtttaataa actcatctgc
     5041 aaaagtaaaa gaaatgggta cacaaatagg taaaggaata gaagattcat taaaataaat
     5101 ggaaataaaa atatttaaaa acttaatgta aatttatagt tgtaaattta tactaaagtc
     5161 aaaaaatgaa atataagtgt atcaatagta agactgtatg tacatttgca catgtgaatg
     5221 caagtatata tacagtcttt ttttgtagtt aaatgcaata atttgattgt gttttgtaaa
     5281 atacacaata tgatataatt agataatata cacttaattt aattgaggaa ggtgattata
     5341 tggaatattt atttgaaaaa agtagtttaa aagaatatgg aaatgaagct ttagataaat
     5401 atagagaatt attagataaa actggtaata attcaaggtg tattcttttg ttagtaccaa
     5461 acaataacac aaagatacga tatgaaaggg ctctaagatt aaattatagt gaagaactga
     5521 aaataacaac atatataagt tttgtgaaga aagaattagt taaatactgg ccactaataa
     5581 tagaaaaatg tgatggaata agtacaaagg ttgtatctcc aacatttata tcaaatagtt
     5641 taagtgaata tttaatagtg caaaaagtta aggaaaagag gatacaagaa ggatattttg
     5701 aagatattac aggaaccaat aaatccatag caaatagtat aatgactaac ataaataagt
     5761 ctgcatttaa tttaattgat tttgatttta ttggagaaaa aatatattca tcaaagaaaa
     5821 ataaagatag tatatataga ttttcataca ctcaaatgga tgaaattata tcatattata
     5881 taaattcctt actatcaaat tcaattttag ataatgcatt aagcatatat ttatacaatc
     5941 aatacctact taatgatgat ttttatttaa aaaacttatt tacagaagtg agatatataa
     6001 ttgtggatag ccttgaaagc tcaagtaatg cagaagttga ttttataaca gaagcattgg
     6061 ataatacatt acaaagttat atatattttg attatagtag ggattattct gtatttaata
     6121 atgtggatat agactatata aatgagaaaa taatatcaaa aattaagtct aaagaagaaa
     6181 atattaaaaa gaaggagatt agaatagaag atttatactt attacctgca aatatagaat
     6241 taaatcaatc aagtcaatta tataatgaaa tgttagattt aataagtgaa aaagttatat
     6301 ctcttataga agaagatgta tctccaagag atatagcaat tatatctcct ataaacaata
     6361 gtatattgga gcatcaaatt agagattctt tgatagaaaa aaatatagat gtttttagta
     6421 ctaagaagga taaaaaagct gtggattatc catatgggaa tgcattggta gtggctacat
     6481 gtatatttta tggatattta gattttataa aagaggatga atacttaagc tttttagaaa
     6541 ctcttttaga aataaatagg ataaaagcat ttaagatttt taatgaaaca agacatttaa
     6601 gtattgatga tatagatgaa catagtcagt atcgtgagat tttacagtat attgaagaaa
     6661 aaaagaagag tgatataaaa atacatgaat ttttaactca attttacata gataaaatgt
     6721 taaatttaaa agaaggtaaa aagaatgttg ggttatgtaa aaagataata gctgaaagtg
     6781 agtcttttag tgaaagttta agtctacttg ggatgaaaaa agaaaagata tttgtagaag
     6841 ctttaaaaag tacaataaac gattattact ctgttgtcga tatagaagag ttgaaatcca
     6901 aagacaaaat agtaataact actccttatt catatatatc atcaaatata gatagaagta
     6961 tacaaatatg ggtagatata ggaagtaatg cttggaatat gaaaatagaa aaagatataa
     7021 gtaatttaat tgtacttaga aaatcttttg aagaaaaaaa gatttata
//
LOCUS       DAEMIK010000177         7055 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEMIK010000177 DAEMIK010000000
VERSION     DAEMIK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.180"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..1784
                     /locus_tag="KPK87_003381"
     CDS             84..1784
                     /locus_tag="KPK87_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539493.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2147..3334
                     /locus_tag="KPK87_003382"
     CDS             2147..3334
                     /locus_tag="KPK87_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539494.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            3394..4557
                     /locus_tag="KPK87_003383"
     CDS             3394..4557
                     /locus_tag="KPK87_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2539495.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            4708..5511
                     /gene="zupT"
                     /locus_tag="KPK87_003384"
     CDS             4708..5511
                     /gene="zupT"
                     /locus_tag="KPK87_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG2539496.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            5606..6166
                     /locus_tag="KPK87_003385"
     CDS             5606..6166
                     /locus_tag="KPK87_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG2539497.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            6417..>7055
                     /gene="rgbR"
                     /locus_tag="KPK87_003386"
     CDS             6417..>7055
                     /gene="rgbR"
                     /locus_tag="KPK87_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG2539498.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEE"
BASE COUNT         2675 a          737 c         1308 g         2335 t
ORIGIN      
        1 aagttattgt tatgttatac taatttatgg gtatttttta gaagtactaa tagaagtact
       61 aataaaggag ggatttaata cttatgataa aacaatttgt caaatattat aagccatata
      121 agaaaatttt tacactagac cttatagcag cttttctatt ttccttatgt gatttagttt
      181 atcctatgat aactagaaat ataatggatg acgttgttcc aaataaaaac ttaagaatgt
      241 tagtagtttt tgcagtagca cttatactta tatttatagc aaaagcaggg cttaactatt
      301 ttatgcagta ttggggacat gttattggtg tagatatgca agctgatatg agaaatgagg
      361 tatttacaca cttacaaaga cttccaaata cgtattttga taataacaaa tctggagtaa
      421 ctatgtctag aatagtaaat gatttaatgg atataacaga acttgcacat catgggcctg
      481 aagatttatt catttctata gtaatgttgg ttggttcatt ctttatactt atagatataa
      541 atattcctct tacgcttata atatttgcta tattaccgtt tataatttgg tttgcaatag
      601 ctaaaaaaga taagatgaat attgctttta tgaagagtag agttactata ggtgatgtaa
      661 atgctacttt agaaaatagt atagcaggta tgaaggtaac taagtctttt tgtactgaga
      721 aagaagagtt aaataagttt gtaagaagca ataaattatt tagaagagca agacaagatt
      781 cttataaggt aatggcagaa tattactctg gtatgaattt atacatggat atcttagagt
      841 gggtagttgt aatagctggt ggttatttta cttatatagg aaagattact ttgggtgatt
      901 ttgctgctta tatactatat gtaaaaatgt ttatacaacc tatgaaaaaa ttaataaact
      961 ttacagagca atatcaaaat ggtatgacag gttttaaaag attcatagag ataatggaac
     1021 aagaccatca aaaagaagct aaaaatccta ttgaacttga aaatgtaaaa ggtgacattg
     1081 agatagaaaa tatatctttc acatatgaag ataagactca agttttggat aatttaagtt
     1141 tatctataaa agctggtaag actatagcat tagttggacc ttctggagga ggaaagacta
     1201 cattatgtaa tctattgcca agattttatg aatttgataa gggtgatata aaaattgatg
     1261 gaaagagtat aaaagatgtt agtttaaaat cattgagaaa aaatataggt atagttcaac
     1321 aagatgtatt tttatttaca ggaactataa gagataatat tctttgtggg aaccctaatg
     1381 ctacagatga agaaatgata gctgctgcta aaaaagctag aatacatgac tttgtagaga
     1441 ctttgccaga tggatatgat acttatatag gagaaagagg cgtaaaatta tcaggtgggc
     1501 aaaaacaaag aatttcaatt tcaagaatct ttttaaagaa tcctccaata ataatattgg
     1561 atgaggctac atctgcttta gacaatgtaa cagaaagaga aattcaagag tctttagagg
     1621 aattaagtaa agatagaaca aatttagttg ttgcacatag attgactact ataaaaaatg
     1681 ctgatgaaat catagtattg acagataaag gaattgaaga aagaggtact catgaagagc
     1741 tagtaaacaa aaatggagta tatagtaggt tacacaataa ctagtataca aattttacac
     1801 catagctaac atacacatta aacttacaaa cataggatat attaagattc ataaagacaa
     1861 ttcattttat atttataaga tgttttatga ataaaatgta tcctatgttt tattttttgc
     1921 aaattacatt taattctctt tgtaagaata gagttaaata gccaatggtc gggataacgt
     1981 ttttacaaaa agaataattt agaaaactaa ctttaattta tggaatttat cgaattttat
     2041 gaaaaaaatc gaattttgac ttgaaatgtt tttcaaaagt agtaaaataa tggataacta
     2101 tacatacatt ttataaaaat atatttatta tgagggggga ttttgtatgg aggcaataac
     2161 agctttttgt ctagttttag tatcactgac gataggagat atagtttcag caaaaactaa
     2221 agcttttgtt ccgtcggttt ttgtatcagc aattatattt atagtaggat tttggacttt
     2281 tttcccagaa aatattgtgg acttagccgc tttaggaaca ccattagctc aattaggtat
     2341 gcttttgtta ataactcata tgggaactat gatgagtatt aaggaattag caggtcaatg
     2401 gaaaacaata gtaatagcct tggcaggaat agtaggaata tgtgtaggtg ctttggcatt
     2461 agggacagta gtatttggat gggatacagc agtaatagct acacctcctc ttactggagg
     2521 gctagttgca tctataatga tggcagatgc tgctacagca aaaggtcttg caagtttatc
     2581 agtacttgca attttgatgt atgttgcaca aggatttgcg ggttatccta ttacagcatt
     2641 aatgcttaaa aaagaaggaa aaagactttt atcagacttt agaagtggaa aagtaactgt
     2701 aaatacagat gaagaaaaag ttaaggattt acctgaacaa aaaagcagat ttaagataat
     2761 tccagactta ccagaaaaat atgatacgac atatatgata atacttagat taggatttgt
     2821 agcatggtta gcagtacaat ttacaaatct tacaaatgaa gttgtatcaa gatatgttat
     2881 atgtttaata tttggtgtaa tagcatcaga acttggaata gttgatagaa aaccacttaa
     2941 taaatctggt tcatttggat ggttaatgac atcattgatg gcttttatat ttgcagggtt
     3001 agcaaaagct acaccagcaa tgcttgttga aatggcagtt cctcttgtag gaatcataat
     3061 atttggtgta ataggtatgg gaatattctc tattatagta ggtaagcttt taggtgaaag
     3121 taaagaaatg gctttttcag tttcgcttac atcactttat ggttttccac caaactatat
     3181 attaactgaa gaggcagtaa aggctttggc tgaaacacca gaagaaaaag aatttttaat
     3241 ggataagatg cttccaaaga tgcttgttgg aggatttact acagttacaa tagcttctgt
     3301 agtaatagcg ggtatctttg taaatctaat atagtatttg agtgataaaa agtattttaa
     3361 ataagaagaa tgtgtactac ttggaggtat ggaatggata ttaaagaaat aacaaagagt
     3421 tataaagact atgtaattaa gcttagaagg gaatttcatg aaaatcctga gaaaagtatg
     3481 gaagaagtta gaacatcaaa gagagttaaa gaagaacttg ataaaatagg gataccatat
     3541 gtatcagctg gaggtacagg tgtaattgca actataaaag gagctaatcc aggaaagact
     3601 gtagcactta ggggtgatat ggatgctcta caagttgttg aatgcactga tgtagaatac
     3661 aaatctaaaa atgaaggact tatgcatgct tgtggtcatg atggtcacac atcaatgctt
     3721 ctaggggcag caaaagtgtt aaatgatatt aaggatagta tcaatggaac tgtaaaatta
     3781 ttttttcagc caggagaaga agttggtaaa ggtgcaagag ctatgattca agatggtgct
     3841 atggaaggtg tagatagtgt gtttggaata catctttgga ctgatgtaga atctgggacc
     3901 atatcggtag aagaaggacc aagaatggct tctgctgatt tctttaaaat tactgtaaaa
     3961 ggtagaggtg gtcatggttc acttcctcat caaggtgttg atgcagtgct tgcaagttca
     4021 gcaatagtta tgaatctaca atctatggtt agtagagaag taagtcctct ggaaccatta
     4081 gttgtgagtg ttggagtttt aaattctgga actagattta atgtaattgc gagtgaagct
     4141 atcttagaag gtacaataag gctatttaat ccagagctta gaaagcaaat tcctggaatt
     4201 ttagaacgta tagcaaaatc tacagcagaa gcatatagag cagatgctga attagagtat
     4261 gggtacttaa caccagcagt tataaatgac aaagaatgtt caaagatagc tacagatgca
     4321 gcaattaagc tatttggaga agactgtata acactatttg aaaaagtaac aggagcagaa
     4381 gatttagctg agtttatgaa tatagcacct ggtgcactag catttgtagg agctagaaat
     4441 gagtcaaaag gtgcttgtta tcctcatcat catggatgct ttaatataga tgaagatgct
     4501 ctagaaattg gaactgcttt atatgtgcaa tatgcagtgg atttcttaaa taaataattt
     4561 taatatacta taaagaaaaa gcagagacta agtttatatc tctgcttttt tattgtgtat
     4621 attttttata aatattgact atataaaata tatatgatag cataaagaaa atgataatca
     4681 atatcaatta aaaagaaggt gatttatttg aataatgtaa tatttgcatt tttgataaca
     4741 ttattggctg gtttatctac tggtatagga agttgtatag cattttttgc taaaaaaaca
     4801 aatactaagt ttctttctat aagcttagga ttttctgctg gtgttatgat ttatgtttct
     4861 atgatagaaa tttttcctaa agcacaagat gctttgacaa aatctatggg agagaaatta
     4921 ggaagttggt caactgtaat agcttttttt ataggtatgg ctatcattgc tgttattgat
     4981 aagctaattc cacaagaaga aaatccacat gaaataaaga aaatggagaa tatagaagaa
     5041 aagaatatca aaaaaaataa gtctctttta agaacaggta tatttacagc aatggctata
     5101 gcaattcaca attttcctga aggattagct acatttatat cagctcttga tgatgttaca
     5161 atagctattc cgatagcaat tgcaattgca atacacaata taccagaagg tatatctgta
     5221 tctgtaccag tttattatgc cacaggagat aaaaagaaag ctttttatta ttccttttta
     5281 tctggtatgt ctgagccttt aggagcgatt attggatatg tacttcttag aaatttttta
     5341 aatgatataa ctcttggaat tgtatttgca attgtaggag gaattatggt attcatatca
     5401 ctagatgaat tattgccatc agctagagag tacggggaac atcatttatc tatttatgga
     5461 ttgattgcag gaatgggtgt tatggctatt agtttattac tatttaaata gcttttaaat
     5521 atatgattgt aacctaaaat tagtatcaaa atgatataat tatttaaata gatttttaag
     5581 ctatttaaaa atatcatttt ggagaatgag tgctatggaa aagaaaataa aattcaaatt
     5641 tgactggtat agttcacctg ataagataga gaaatggtta gaagatattg aagctaaagg
     5701 atataattta tataagataa ataaatttgg aggaatattt tattttcata agggaagtcc
     5761 aagaaaaata aaatacactg ttgattacta ctatgatgta gagaataatt attttgatga
     5821 acataaatca aatggctgga atcttgtatt taggagtatt ggtagtttta tgggtacatc
     5881 tgcatggtat atatggtcga aagaatacaa agacatacct cctaaattat atagtgattc
     5941 tgataaaaca aataaagtaa agtcaatttt atttatgaat atgtttatat tctttatgtt
     6001 agtagtaata tttatatatt tattgaatat tgaaataaga aatttatata gtggttatgg
     6061 agcatctgta actgctattg tactacagat tatattgttg attttatatg cagtacaatt
     6121 atataggaga ttaatgtatt atttaaggct taaaaagttt aaataaataa aagaaggtgt
     6181 cctaaaatta tttttttaat attttaagac atcttctttt gttaataaag tactgttttt
     6241 aattatagta atctctgtct ttaaattgaa atatcaaaga atagttatat gggattgagt
     6301 attgataaaa ataatttgaa aatgttatcc taaagtatca gatatacaaa attatcttta
     6361 aatatattat aaatatttta attgtattaa ctagaaatta tgtaaggagg cattttatga
     6421 ttaagatagc agtttgtgaa gatgaaaaag aaacacaact tttaatagaa gattaccttg
     6481 agaatatatt aaaagatata agtatagaat atgagataca aaagtatata tctggagaag
     6541 agttactgga aagtaattta aaagatatag atatattact tcttgatata aaaatggaaa
     6601 aactaaatgg aatggatact gcaagaaaga ttagagaagt agacaatgaa atggaaataa
     6661 tatttgtaac ttcattaata gattatgtgc aagaaggtta tgaggtaaga gcttataggt
     6721 atttactaaa gccaatagag ttggaagaac taaaaaaaca tgtgctaact tgtatcaaag
     6781 atattgaaat aaataaggaa agtcatatta caataaaaaa taagtctaat acatataaga
     6841 tttatttaaa tgaaataaaa tatatagaag ttcaaaaaaa agatatgcta atacacacaa
     6901 taaacaagaa ttttgatata aaatatagtt taggcaaaat agaaaaagaa ttaaatccat
     6961 ataaatttat aagatgccat aaaagtttta tagtaaattt aagatatgtt gaaaatataa
     7021 agcctaatac tgcaatactg gaaagtggag aagag
//
LOCUS       DAEMIK010000178         6994 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEMIK010000178 DAEMIK010000000
VERSION     DAEMIK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.181"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(35..2053)
                     /locus_tag="KPK87_003387"
     CDS             complement(35..2053)
                     /locus_tag="KPK87_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2539499.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(2323..3099)
                     /locus_tag="KPK87_003388"
     CDS             complement(2323..3099)
                     /locus_tag="KPK87_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG2539500.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(3156..4127)
                     /gene="asrC"
                     /locus_tag="KPK87_003389"
     CDS             complement(3156..4127)
                     /gene="asrC"
                     /locus_tag="KPK87_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG2539501.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(4140..4931)
                     /gene="asrB"
                     /locus_tag="KPK87_003390"
     CDS             complement(4140..4931)
                     /gene="asrB"
                     /locus_tag="KPK87_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG2539502.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(4932..5939)
                     /gene="asrA"
                     /locus_tag="KPK87_003391"
     CDS             complement(4932..5939)
                     /gene="asrA"
                     /locus_tag="KPK87_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG2539503.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVTSKEE
                     V"
     gene            complement(6329..6982)
                     /locus_tag="KPK87_003392"
     CDS             complement(6329..6982)
                     /locus_tag="KPK87_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2539504.1"
                     /translation="MIFNNLSTKTKNQLKEKVKLKRLQKKEILFYEKDSLDSVYILLE
                     GKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFENSIVAYYSIEEFL
                     EIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYRLSREFGVCCGEW
                     TLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVKETELSRYFKNN"
BASE COUNT         2305 a         1256 c          607 g         2826 t
ORIGIN      
        1 attttaaata tatggctatt ctataattta atatctaagc tgcttctttt attgaaactt
       61 tagttatatt acttattcta tacatttctt tgacaccatt tagagtttgc agtaagttta
      121 gacatattat cttgtcacct agacttttag tatttacaat actctctaaa attacacttc
      181 ttccttcatc atacttatct acattgtaat ttgaaatata attagtatct ctcaatattt
      241 tttcaacatc ttcatctttt attttatttt tattaacatt tatatataga ttatacatat
      301 tacttattag taatttctga ttttctaaaa aatcctcttg atgtttatct ttatatatag
      361 catttatcaa cataagtcta tcttcaataa gtgatggtat caataataaa ttttttatat
      421 agtaaacatc agctacattt tctatagact tattgacttc atcaattata tttttaacta
      481 ttcctttata catttctata acatgttgat agcctgtttt agcatcatat ggtaatataa
      541 acttatttac tattaatgca ataattgctc ctaatgctac atatgaaatc ctgcttatgg
      601 ttaaaacatt tgggtctccc ataactactg ctgaaccaag agctgaaaca gttacacata
      661 ccattaattt tctataatca actacataag agtttatata ccctgctaaa agaactatta
      721 acgaccttaa tgtactatct tttattattg aaaataatac aataaatatt acagctccta
      781 taaatgtacc ttctattctt tttctagacc tttgtataca attttctgcg taaggttgtg
      841 ttaatgaaaa gacagttaac attatccatc ttccatctct taaatggaaa taatccataa
      901 taaaacccgc tacagctgta gctaaagcaa tctttactgc ataagaaaat cttatagaat
      961 ttaaattaaa gtttttctta tatattgata gtcgtttaaa ttcatgaggt atctttgatt
     1021 ttctttcagc tttcttttcg tctcttgatt ttactttttt ataatctact aaaaactcat
     1081 ataaattttc taaaacacta taaatttctt ttaaatttat atttttagta tcctgagcaa
     1141 taacataatc tctaaatcct aatatctctt tatcagttat ggtatcttta ttttctaaac
     1201 acatcttaat atagtttatt tctttcgttg ctacaactaa aacatctgat ttactattta
     1261 tgctactttc ttctaaaata ttttttatat cttcatcttc aaattcttta ctatcctttt
     1321 tgtatctttc caggattaaa cttattcttt ctaatgaaaa taaaatattc attatgtcta
     1381 tacctctaat actgatataa aagtcatctt ttctattatc aaaaataacc attttcaaat
     1441 ttttagatat agaatttatt ctataattac agctatctat ttttctcata ctagatttaa
     1501 ttatagaact atcatcatta atagtatttt ttaaaagact aattttttct aataattcat
     1561 cacatataga tatcagttct ttatctccga catttttcat cctatttcta ttcataacaa
     1621 attgagcaat cattattata actgctccaa cagctaaagc cgacaatctc ataggaaatt
     1681 cactttttcc tacaggtata cttaccataa atatatacat tagaccaaaa ggtacatata
     1741 ctgctctttt taagtcataa ctaaatacat accctattat aaataatgct ataaaattta
     1801 ataaaactcc taaatacata ttttgtactg caaaaaatga tagtattcct aagattatat
     1861 ttattgctaa atacttcagt aaatttttaa ttggagaaat agttaaatct ctttccaaga
     1921 gtgttagcat agctgttact atacctacac ctataagtgt atttttactt ccaaataaat
     1981 atataaatat agaaataaat gctattataa aaataaacaa ctttgttttt gatattacta
     2041 gtttctttgt catcatatcc cccctaaaaa tttcaacaag accaatttta tcatatttgt
     2101 tatcttattc aaaatgcgct tttttacggt aggactttaa ctttttttat atttagaact
     2161 atataattca tatataaacg tattttttat atatataacc gatatatttt cgtatgctcc
     2221 catatttttt tgtttcaaat attttttcaa aaatataaaa ataactctct caataatact
     2281 aaaaatgcat ttatcaagag agttataatt tatacaataa atttaattaa gcttttcttt
     2341 acctaatata taagttccaa gacccataaa taaagctcct ccaacaatat ttccaagtgt
     2401 tacagctact aaattataaa tagctcctcc aatagtaact gtacttattg ttggtgaaaa
     2461 taatgaaaca ctataaattg tcatatttgc tacactatgc tcaaagcctg atgttataaa
     2521 agcaaataaa cataagaata tcataattat ttttgcagta tcactattag ttctaaacga
     2581 acacaacact gatacacata ctagaatatt acataaaatc cctctaaaga aaagagctgt
     2641 aaatggcatg gatgctttac tagctgctgt atttgcaaaa aattcagcta caggtccttt
     2701 gtctactaga ccagttccta caaatataat gcctaaaacc aaagctccga ttaaattacc
     2761 tacccaactg tatgcccata ttttagaagt atcttttata cttacacctt tatttaacat
     2821 accagcagac ataaccatat tgtttccagt aaataactct gttcccgtca ttataaccaa
     2881 acttagtgct attgcaaatg ataaccccat tactatctta gtcataggtg aacctgcatc
     2941 agtcaaaagt cctccaatag taaatatcaa aagtatacct atacctacat aaagtcctgc
     3001 aaacgcagaa gataccaaat attttacttt acttgtgttt aataaattta tcttatttat
     3061 agctgcatta gttaatttat ccaaagtttc tttatgcata attccctctt ccttctatta
     3121 aattttaatt tattcaaata tatgttttac tcaatttata gatatttaat tccactccag
     3181 tatatgtttt cgtacatttt agttatttct ggaagctcta caccttcaag tgcccatttt
     3241 ttaaattcca taaatcctgt tctatcaatt atatatccta tatgttcttt tcctcctggt
     3301 gcatctttat ctatatactt ttctacatat ctataagtat taagtattat ctttattatg
     3361 ctgtcctcat ctacccataa aagaaaatct tctgctagtc tagggttttt ctttcctgtt
     3421 ctacccatta tagctaatct atagtatttt ttctcatctc tagtccatgc atttgttggg
     3481 caatttagta cacactctcc acagcctata cacttatctt tatctcttac caccttatta
     3541 ttttctgttt ttaatgctcc aacagatatc ttagaacatt tttttacaca tgctccacaa
     3601 tttacacatc tatccttttc atatattggt aaagccatac caataattcc aaagtcattc
     3661 attctagctt ttatacaatc atttggacaa ccagttaaag ccactttaaa atgtaaatca
     3721 tttggaaata tagctttttc tattctttta gcaaattcag ttgtattata ttgagccttt
     3781 ggacagactt tatttcctat acaagctgct atatttcttg tacctgctgc tggataacct
     3841 gcgtttttct cactttgatt tatattcatt ttttcaataa caggttgtat aattttattt
     3901 atttcttcca tatcttccat atttattcct aatatttcaa acccctgtct tgtagttatg
     3961 tgcacctgtc cattgccgta ctcacttgca attttagaaa ctacagctaa actctcaggg
     4021 tcaataagac caccaggtac tctaactctt aaagaagtct catattttgt ttttgtaatt
     4081 ctataagcat ttttcattac ttttttggta tttaaatctc ttatcacaaa cttcaccccc
     4141 taatcaataa gatttttagc atatgaataa tcaaatactg gtccatctaa acatatgtaa
     4201 gtatcatcca ttttacaatg tccacattta cctattccac aacacatttt tctttcataa
     4261 gaaacccaaa tgttcttttc atctaaatct ctttttaaaa attcttctac tgtaaatttc
     4321 atcatcattg gaggtccaac tacaactata gaagtattgt ttatatcttt catatccaat
     4381 tctggtatgt attttgttac taatcctaca ttaccactat atccttcctc tgctccatct
     4441 acagtaacta aaatatcaag ttttttactc catctcttta aatcatctgc aaataaaata
     4501 tcttttggtg atttaaatcc tactattaat ttaaactttt cgcatttttc tttattatta
     4561 taaaaatatt caactattcc tcttacaggg gctaatgcac ttcctcctgc aactactaca
     4621 atttctcttc cttcataaag ggatacatca aatccatttc cataaggacc tcttataaaa
     4681 aaagaatctc cttctttata attaaacaac tcgctagtta cttttcctac atttcttatt
     4741 gtaaagtcaa tataatcctc tccatatcct gatactgata ttggactttc accgtattta
     4801 ggtatagata tctcataaaa ctttccaggc aatacacctt ttgtattgca gtttactcta
     4861 aaagtccatt ctttatcagt atgtttaact atatctagta tttttgcact tactggtata
     4921 tatgggttca tttatacttc ctccttacta gttactaaat catttatttt ttctatacac
     4981 tttgaaaatg atatgtattg cggacaggca tcatcacatc ttccacagcc aacacacata
     5041 tgatatccga atcttttttt aaaatcatgt attttatgca tagttttaaa tctcattctt
     5101 tcgccttgtt tttttctaaa agaatttcca cctgctatat ctgtatatcc atctacttga
     5161 caagaagccc aaacccttct tctctcacct acattttcgt tttccttata gaaaatatcc
     5221 tgcattgtaa aacatgaaca tgttggacat acaaaattac atctaccaca tcctatacat
     5281 cttgtatcat attcatccca tattgaattt cctatcattt catttgaatc aatattctct
     5341 ggcacatcta cagatattaa attatcagtt acataatcaa cattaaattc agttgcttct
     5401 ccattaaata cttcaaattc actatccttt atatctaagt acagtgtatc tccttctata
     5461 tttaatccaa ttgaataatt atcagttttg ttagaatcca tgctcacaca aaaacagttt
     5521 ctaaaacttt ccttacaccc tacaagtgca aactttactt tatctctgat atttttataa
     5581 aaataatctt tttcttcacc attatttaga tatatttcat caagtctttt tataccattt
     5641 atgtcacaag ctcttaagaa aattaaaatt ttcttgttgt ctaaatcgct ttccttaaac
     5701 tctttttcag taaaataaaa caatacttga tttatgggaa gtacaacttc ttttggtgaa
     5761 aagtttgctt tcttagcaaa ttccatttct tcaaaagcgt ttacctcctt gtatcttata
     5821 acatcagtat ctgaaaaagt acctttaaat gggattaaaa ctggtgcaaa tatcttgtag
     5881 tcttttttta gataattcag accttcattg aacttagatg aatctaattt tagtttcatt
     5941 tttatcacct ttcttatatt ttagttttac ttacttgcta tcttgtttaa atcttaacac
     6001 tttattttct atgcttccgt gataattatc acagtttcaa aaataataaa aaaaatatta
     6061 atggaattta ttaagttctg ttaatatttt tttattatct acattattcg atttcaaatc
     6121 taatcactta aatacaatca ttaagtacaa taaaactact atctactcaa ttttttgaat
     6181 aaaaaagaat cttatttgtt ttttatacaa ttcaataggg taaaataaaa attaataaat
     6241 tagacaataa aaaagaatct ataaatagat tctcttaata atatttttat ttaattaaaa
     6301 ttctaataat actcttactt aatcataatt aattattttt aaaatacctt gataattcag
     6361 tttctttaac ataaaaacct ttattgtcaa ttttaataag ttcttctttt tgcaatacct
     6421 ttatcattct ggaaacagtc tcccttttac agccaagcat atcagctaaa tatgtaactg
     6481 taatatttac atttagtaaa gtccattctc cacagcaaac tccaaattct ctacttaacc
     6541 tatataattt tgctgcaagt tttttatcta cttttatgga tatagaattt ttcaactgtc
     6601 tatataaacg tcttattctt ctttccatat aagaaataat atcctttgta agttgaaaat
     6661 cattttgcat aatttctaaa aattcttcaa tactataata agctacaatt gaattttcaa
     6721 atgcttcaca gccaacagta gagtttttag aagcgtcaag tgtaatatca tttatgactt
     6781 ccccactatt taatatgaaa attacttttc tctctccatt ttcacttatc ttaaaaatag
     6841 atacctttcc ttctaaaagt atataaacac tgtctaagct atctttttca taaaataaaa
     6901 tttctttttt ttgtaatctt tttagtttaa ctttttcttt aagttgattc ttcgttttag
     6961 tacttaaatt attaaaaatt attactttat caat
//
LOCUS       DAEMIK010000179         6895 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEMIK010000179 DAEMIK010000000
VERSION     DAEMIK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.182"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1384
                     /locus_tag="KPK87_003393"
     CDS             <1..1384
                     /locus_tag="KPK87_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG2539505.1"
                     /translation="LNIGVRACLYFAMFSIICILINILISILIVGFNSRSSKYVLSLN
                     GKKSYGFVNIMHFTLKIVFVSFLILSINNCILNYNMLQTQLGNLSEWDKAKNVYNLTL
                     KDTGEQSLGKEEVIKNAKIKGFYKNLVEEKDAFLIDSTNFEKLEDGSYMYEINSKGKN
                     PEVSQYGKNIKINKNYLKVNPIYSNGKEIFNLIDYDDKTINLLVPKKLQVHENEIKKE
                     FRELFYFEKIEVENMYNKELNRELNKTKKSDLNVNIIYVDNNQSYFTYNSLVMDDNRN
                     LIDDPIAIVDIDNIDDSFYLSYISRCVYFNSKKLDALADISNIIEAQGVEAHIQSLHA
                     VYNEYGLEINKLEKYLNSEIFTIIIIAISNLMITYNIVASYYERNKYKLYIKKIFGYS
                     TTARSMLLIVSLILTNIIPIGIISTRVDLSNNIILFGLLILVIEVIVSIVLDKIISNS
                     SFNKIIKGEH"
     gene            1389..2006
                     /locus_tag="KPK87_003394"
     CDS             1389..2006
                     /locus_tag="KPK87_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539506.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            2195..2539
                     /locus_tag="KPK87_003395"
     CDS             2195..2539
                     /locus_tag="KPK87_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG2539507.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            3109..3975
                     /locus_tag="KPK87_003396"
     CDS             3109..3975
                     /locus_tag="KPK87_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2539508.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            4088..4492
                     /locus_tag="KPK87_003397"
     CDS             4088..4492
                     /locus_tag="KPK87_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG2539509.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLVCGETTVNDGHCHKFLFTTQIEAPLV"
     gene            4668..5117
                     /locus_tag="KPK87_003398"
     CDS             4668..5117
                     /locus_tag="KPK87_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539510.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(5273..5650)
                     /locus_tag="KPK87_003399"
     CDS             complement(5273..5650)
                     /locus_tag="KPK87_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2539511.1"
                     /translation="MLTPYLIFNGDCEKAFNFYAEAFGGGKTIFARLDSNPNNPVMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            5912..6829
                     /locus_tag="KPK87_003400"
     CDS             5912..6829
                     /locus_tag="KPK87_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG2539512.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT         2798 a          716 c         1090 g         2291 t
ORIGIN      
        1 attaaatata ggtgttagag catgcttata ctttgctatg ttttcgatta tatgtatttt
       61 gataaatatt ttgatatcta tattaatagt tggttttaat agtagaagtt ctaaatatgt
      121 gctatctcta aatggaaaaa aatcttatgg ttttgtaaat ataatgcatt tcacattgaa
      181 aattgtattt gtatcatttc ttatattatc cataaataac tgtattttaa attataatat
      241 gctacaaact cagttaggaa atctatctga gtgggataaa gcaaaaaatg tttataatct
      301 tactcttaaa gatacaggcg aacaatcttt aggaaaggaa gaagtaatta aaaatgccaa
      361 gattaaaggt ttctataaaa atttggtaga agaaaaagat gctttcttaa tagattcaac
      421 taattttgaa aaacttgaag atggaagtta catgtatgaa ataaattcaa agggtaaaaa
      481 cccagaagta agtcaatatg gtaaaaatat aaaaataaat aaaaactatc ttaaagttaa
      541 ccctatttac tcaaatggca aagaaatatt taatctaatc gattatgacg ataagactat
      601 aaatttatta gttcctaaaa agttacaagt acatgaaaat gagattaaaa aagagtttag
      661 agaattattt tattttgaaa aaatagaagt agaaaatatg tacaataaag agttaaatag
      721 agagttaaat aaaacaaaga agtcagattt aaatgttaat ataatatatg tagataataa
      781 tcaaagctat tttacttata actcccttgt tatggatgac aataggaatc tcattgatga
      841 cccaatagca atagttgaca tagataatat tgatgattct ttttatcttt cttatatatc
      901 tagatgtgta tattttaatt ctaagaaatt agatgccctt gcagatataa gtaacattat
      961 tgaagctcaa ggtgtagaag cgcatatcca aagtttacat gctgtttata atgagtatgg
     1021 tttagaaata aataaactag aaaaatattt gaatagtgaa atttttacta taataataat
     1081 agcaatatca aacttaatga ttacctacaa tatagtagct agctactatg aaagaaataa
     1141 atataagtta tatattaaaa aaatatttgg ttattcaact acagctagaa gtatgttgtt
     1201 aatagtctca ttaatattaa caaacattat tccaataggc attatatcaa caagagtaga
     1261 tttaagtaat aatattatct tatttggact tctgatatta gttatagaag taattgtatc
     1321 tattgtatta gacaagatta tctcaaatag ttcatttaat aaaattataa aaggagaaca
     1381 ttaaaaagat gatttcatta ataaatatat ctaaaaaatt taaagataaa tctataatag
     1441 acaaatttaa cctagaaatt tctaagggcg agtttgtagc gataacagga aatagtggtt
     1501 caggtaagac aactttactt aatataatgg gattattaga aaaacctaat tctggtgata
     1561 ttataataaa taatataaaa aatccaaata gtaaacagat aagaatttta caaagggatt
     1621 tttatggcta tctatttcaa aattatgctt taattgagaa tgaaaatgtt gaaaataact
     1681 tgaaaattgc tttaaagtat caaaaaaata tcaaccataa tgaaaaaata aattacgcac
     1741 ttgagtctgt aggtcttaga aaatacaata aaaaaaagat atacgaattg agtggaggag
     1801 aacaacaaag ggtagcatta gcaagagtaa tattaaaaga atgtgaagta atatttgcag
     1861 atgaacctac aggtaattta gataaaaaaa atagagacat agtatttgat atacttaaaa
     1921 gcctaaataa gaatggtaaa actatcgtat ttgtaactca tgatttagag ttatcggagc
     1981 aggcagatag ggttataaaa ttgtaataat ataaactata ataaatataa gaaagagatt
     2041 acagctaaat caagtttaat aataatacga atagaattta aacattaagt aaattagtag
     2101 tttgtaaata taagtataat atgatatcat aaaggctcaa aagaaatttt atataataag
     2161 aaatctaaaa ataacaaatg gaggacatat aaagatggaa aatttaccaa attgcccgaa
     2221 atgtaattca gaatatactt atgaagatgg aactctttta gtttgtccag aatgctctca
     2281 tgagtgggca ttaggattgg gagcagaaga agattcaaat attatcaaag atgtaaatgg
     2341 aaatgtatta aatgatggag attctgttac agtaattaga gaccttaaag taaaaggaag
     2401 ttcatcatct ataaagattg gaacaaaagt gaaaaatatc cgtttaatcc atgactcatc
     2461 agatggacat gatatagaat gtaagataga tggttttgga gctatgaagc taaagtcttc
     2521 tgttgttaaa aaagcgtaat attataaata agctgtagtg tttattgttt ttcagtacag
     2581 tataaaacca taagaatcta ttaagtaata aaagtttgtt tatttatctt tatattttct
     2641 tatggtttta ttaaatataa attttattga acaccaaaat attctctcct ctacaaataa
     2701 gccaatctta tttaaattaa tatactttgt actatttata ctgaaattgt aaatttaaat
     2761 actatgttat ttaggtttgt atagttaatt tatatatttc tgattttata aattgtattt
     2821 attattatat ctttattcta aaatcattaa aaatttcaac ttctataaag atttacaatt
     2881 tatcaaatac attatgagta tataaacaat ttattattac agtgctacat ttaaaataag
     2941 tcttagtata aaggtattgt tacaagtata aagaaactta tgtatgctat tggaataaat
     3001 aataccttaa attataatta agaataagaa ctgttaaata agggacttga ttatcattta
     3061 gttatagata atataatatt aaaaataaag tttaagagga gaaaaaatat gatagatata
     3121 cataaaaata tatatgataa taagttattt gaggagctaa agattgactg taagaagtgc
     3181 tttggacttt gctgtgtagc tctttatttt tctgcttcag atggatttcc tatcgataag
     3241 gaatcaggaa aaccttgtat taatttacaa ccagatttta aatgttctgt acataatagt
     3301 cttatgaagc gaggttttaa agggtgtact gcatatgatt gttttggttc aggtcaaaaa
     3361 gtagcacaag taacatataa ggggatagac tggatgcaat caccagaact aacaaatcaa
     3421 atgtctgaag tgtttttaat tatgagacaa ttacatgaaa tgttatggta tcttaaagaa
     3481 gcatctgtat taaatatatc agatacaatc aaatcaaaaa ttgatttaat aattgaagaa
     3541 actgaaaaga taactaatat gggcccagaa cagataatta atttggatat catatcacat
     3601 agaacaaagg ttaatttatt attatcacaa gcgagtgagt ctgtgatggg aaaggttaaa
     3661 tcatttatca agacttctac tcttaaaaac atgaaaaaat taagtaaaaa tatagattta
     3721 ataggagcag accttagaaa aataaatcta ataggagcag atttaagagg tagatttcta
     3781 atagcagcaa atttaagaaa tacagatttg agtggggcaa atttaatagg agcagactta
     3841 agagattgtg atattagagg tgctaatctt gaaaatagta tatttttaac acaattacaa
     3901 gtaaatactg caaaaggaga ttctagtacg aagttaccag cttcactaat acgccctaaa
     3961 tactgggaaa aataattaaa ttaatacata attttacaaa atatgaactt ttttgacttt
     4021 aattcatatt atacattaga gattctttac aagaatttct taatataata tagggagaga
     4081 tataaaaatg agttatcaaa attataaatg tgaaagagat tatgtgtatg atgattataa
     4141 atacaataag tgcaacaagt gctataatga ttatgaggaa atgacacatg ttcatgaata
     4201 ttcagaaagt gttaagttag cagaagaatg tgaggataga cataaccacc gtgcagcagg
     4261 ggttactggt gaagttatac caataaatgg aggtacaaat catgttcata aaataaatga
     4321 taatgtagat ttccttgacc attttcataa gatatgtgtt acaacaggac cagcaattag
     4381 aatacctgga actgataaac acattcactt agtatgcgga gaaactactg ttaatgatgg
     4441 tcactgtcat aaattccttt ttactactca aatagaagct cctttagtat aaactaatat
     4501 gaattattgt agtaaatttg ctagattata gtatataaat gttaaaaata tataatagat
     4561 aatatttaaa cttaatgagg actatgatat ttaaccttcg tagtcctttc taagttttgt
     4621 ataaagaagt gttatacaat taaaagtgat atagaaagga aatttttatg aaatctggaa
     4681 taattcttga aatgaaacaa ataaattcaa cagaatatga cgaaatgttg caagtctggg
     4741 aatcgtcagt aagagcaact catgattttt taacagaaaa agatattgaa tctttgatac
     4801 cattagttga aattggactt aaagaagtag aaaatatagt ttgtataaaa gataatgata
     4861 taataaaggg ctttatagga atagataaag ataaaattga gatgctcttt atagaagata
     4921 aatatagagg aaatggtatt ggtaaaaaat taataaaata tgctatagat aaatacaatg
     4981 taaaatatgt tgatgtaaat gagcagaata aaaaagctgt aggattttat attcacttag
     5041 gatttaaagt gtttgatagg tctgaaatag atggacaagg taacccattt ccaatactac
     5101 atatgaaatt aatttaaagt tatacttctc acgtattagc aaaaatgttt acttaaattt
     5161 ttatacaaat aaaagccgtg acagtcttag gacacggctt ttttactaca ctattaaatt
     5221 gaaatttatc tgttctatat tatgattatt agacgtagtt tttttatttt aactatgtac
     5281 ttaaatacca agtatacccg tacctatcaa gtacttcagc agcaccatca ttttgatgtg
     5341 gtggtgggtg tggtaaaaat tcttgtacaa gtgtaccacc ttcggcaagt tttacagata
     5401 tttcttctat cgcttctcga gatggtagaa caacacaaat cgccatgcca gaaattacaa
     5461 caggcttgtc tgtatccgca cccattatac aaccttcgta ttttgtgaaa gtaacacttg
     5521 cgtgcataac aggattgttt ggattgctgt ctaatcgcgc aaatatagtt tttcctcctc
     5581 cgaaagcctc agcataaaaa ttaaatgctt tttcacaatc accattaaat attaaatatg
     5641 gagttagcat gaccaatata tcctccttga tatagaataa tttatagttg cacctgataa
     5701 tctaaattat gatttagtaa attgtatcat agggaaacct tgaagtatat aattttttat
     5761 tattatatta tacaaattta ttaatttaaa aaaatgttat atgtacggaa ggtttacagg
     5821 ataaaatata ataaaaagat taaggtgaca ttataaaaaa tgtatattga cattgacgct
     5881 acgtaattgt atatactttg tcttaaagga gatggtaaat atgaatatta tagaaattcg
     5941 ctcagataaa atatacaaga agataatgga tgcaccaata aacaaaaaag aagatatata
     6001 cagatatgaa ttgatgaagc cttttgaatt taagtggaag tgtatgaatg ttccaatagt
     6061 tgctagacag aaaggtggat atgatgtaat tatagcaagt gaaatgttag gggttttatc
     6121 gcctaaggat attgatgaaa agcaaaaaaa gaatataaat gtgttatctg ctgataaaat
     6181 ttgggccact tgtaaagaaa ccatagaaaa ctctataaat gcttttataa aagaagggta
     6241 tgatttaaac attaaggact ataaatattc aatattattg gcgaatccaa atagtcctta
     6301 tacaatatta agtgatggat actggggtga tggtgggatt cctggatata tatttctatc
     6361 attggttcct aatgaatata ctatcaatag attaccagta ttaatagcac atgaatgtaa
     6421 tcacaatatt agatttcagt ttatagagtg gaataataat ataacattag aagaaatgat
     6481 gataaatgaa ggtcttgcag aaaattttgc aacatggatg tttggagagg aaatgttagg
     6541 accttgggtc agtagaacag atatcgaaac attaaatact tatataaagc caataataaa
     6601 aagtgcttta aaagaaactg gatttcaaaa tataacatct tatctttatg gtgatgatat
     6661 agctaaaatg caaggatatt ttccagtagg gttgccttat tgtgcaggat atgcttgtgg
     6721 atattatatg attaagtatt atttagaaaa gacaaataaa tcaataatcg aagcgacttt
     6781 attgccttat agtgagataa tcgaagcagt aaaagagttt tgggaataaa ttttataaat
     6841 tgcataattt gaaataaatt atgcaattat tttattttta agaaaaaata aattg
//
LOCUS       DAEMIK010000180         6853 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEMIK010000180 DAEMIK010000000
VERSION     DAEMIK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.183"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            28..948
                     /locus_tag="KPK87_003401"
     CDS             28..948
                     /locus_tag="KPK87_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2539513.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            1034..3454
                     /locus_tag="KPK87_003402"
     CDS             1034..3454
                     /locus_tag="KPK87_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2539514.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGDEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHNILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            3454..4476
                     /locus_tag="KPK87_003403"
     CDS             3454..4476
                     /locus_tag="KPK87_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG2539515.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDIFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKNWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            4629..5375
                     /locus_tag="KPK87_003404"
     CDS             4629..5375
                     /locus_tag="KPK87_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2539516.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            5461..6711
                     /gene="cme"
                     /locus_tag="KPK87_003405"
     CDS             5461..6711
                     /gene="cme"
                     /locus_tag="KPK87_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG2539517.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
BASE COUNT         2483 a          994 c         1132 g         2244 t
ORIGIN      
        1 cattttacac aataaggagg gctatatatg acaaatgcta aaagtaaaaa agaatatcta
       61 aatcgtattt gcaaagtgca agactatata gaagaacatt tacatgaacc tctttctctt
      121 gatgaacttt ctaatatagc aggtttttca aaatttcact ttcatagaat cttcaaagca
      181 atagaaaaag aaactttggc acaatatgta aataggctaa aactagaaaa tgcaactgct
      241 tttcttattc atagaactga catgactgtt actgatattg cacactactt tggctttact
      301 gattcagcag ttttctcgag agcatttaaa aattactata aaataagccc tataaaatat
      361 agaaacaatt atagcaagaa ttgcaaagaa ccatataaaa tttctcagta caatagaagc
      421 atatcaaaac ctaaatgtaa gaatactaga gaggttaaag gtgaaattga aattctagaa
      481 cttgataata tgaatactat ttacactaga tatactggtt catatggtaa tctaaccagt
      541 actttcccaa aacttttaga acggttattc acatatgcaa gtgagcagaa tctacttgga
      601 cttgaaaaca cacaaatatt agccatttat catgataatc cacaatttac acaggagcat
      661 caattaagga ctagtatttg catgaccatt ccagataaca tagttgtcaa agagaatgac
      721 aatcttggca atatgataat accttctgga aaatatcttg tgggacattt tgatatttca
      781 aaatctgaat atagtgatgc gtgggatttt atgtataatg aatggctatc aaatggtaat
      841 tataagctac gtgcctcctt cccttttgaa gtatatctta acaacccaca caatcaccct
      901 caaaatagac acttggtcga tatatattta cctatagaac ctttttaata acctcaccta
      961 atctattctt gtattaagga ttaatatatc cctattttac ggtttgataa aattgataaa
     1021 atgaggtgaa ccaatgaaag aaataaaaat taatggagca aagatacaca atcttaaaaa
     1081 tatagatgtt tctatcccca aagataagct tgttgttgca acaggtgtca gtggctcagg
     1141 taagtctagc cttatgtttg acattgtatt tgaggaaggg cgtaaacaat atctgcaatc
     1201 tttaggtata cttgcaggta ttgatagtga ggataagttt gataatattc aaggtttagc
     1261 tcccactata gctgttcaac aaaatattat tcgccagagc aatcctcgtt caacagtagg
     1321 cacaagaacc aatattctca acatgcttac actattgtat tcagtggaag gtcaaataat
     1381 gtgtactatg tgtgatactc ctgtaagcga taatctaatt tgcaataatt gtggatatga
     1441 ggaagaacga ttaagaccaa gttatttctc atacaactct tcagatggaa tgtgtatgaa
     1501 gtgctctgga catggtgcat attttgagat taatattgaa aaattagtcc ttgataagca
     1561 tgacacccta aaacaagtct tagatagagc aaagattaca ccaggatatt tgagagtatt
     1621 ttctaaaaaa ttcaacgatt atttagacat acaattctta caactacctg aagaaataag
     1681 aaatgaagta ctctatggtc attatgaaaa tggtaaaaaa agttcctctt tatcaaaggt
     1741 atttcacaat cgttacaaaa ggggcgaaga tttaaatgga gtttatacta tgactgcctg
     1801 ttcagattgc aatggtttta aaattggtga agaagcacgt agagtacttt taaacggaaa
     1861 acatattgga gaacttggaa aaatgacgat tttagaaatt gatgatttcc ttaaactctt
     1921 aataaatcaa ggtaatttaa acacatttgg tacaaatctt gtaaatgata ttttatccaa
     1981 aactcaacat ttaatagaat cacgattagg tcatctatcc ttataccgag aaatgtcaac
     2041 tttgagtggt ggggaaatgc aaagactatt tttaaattca catctcgatt cagagatgga
     2101 ctcattaatt tatgtgcttg atgaaccaac agctggattg catgaatcag aaaaaataga
     2161 catcttaaaa tcaataaaga aacttaaaga tttaggaaat acggtcatag tagttgagca
     2221 tgataaaaac acaattaaaa tggcagaaca tattattgat attggtccaa aagctggtgt
     2281 tgaaggtggt caattagtat atcaaggaga tttagaaggt ctacttcaat ccgacaaatc
     2341 acttactggg cagtatttat ctggaaaata tccaatgcct aatagaacaa gcaataaaaa
     2401 cattacacat actgataaaa taccttgtat aactattcaa aatgcaaata caaataatct
     2461 taaaaatgta actgtatcat tacctttggg tgctatggtt ggaatatctg gaaaatcagg
     2521 tagtggcaag agttcactta tatcagacac cctacttcct cttcttagaa gccaatttaa
     2581 taaccatact atcaataatc aaacaaattc ttcagagagc gaaataggtg atgaagacga
     2641 ttatgcagtt gttgaaacta ttgcagatag actgattggt actaaacata tttctggata
     2701 ttcagagata tcccaatctc ctattggcaa aaatatgaac tcaactcctg cttcttatat
     2761 tggtatatgg gataagatac gtacactatt tgcaggtcag ccagaatcgt tagaacgagg
     2821 atttacagct ggtcatttct catttaactc caaaggtgct tgtcctaaat gtagtggtag
     2881 tggatatgaa aaggtctggt tgggaaataa tctaagtata gaccatatat gcagtgaatg
     2941 tcagggaaaa agatttaatg atgaatcttt atctattaaa tataaaaata aaaatataca
     3001 taatatttta aatatgagtg tttctgaatc tgtacatttc ttcaaggata ttccaaatat
     3061 tgtttcacac ttaaatgtat tagaacaaat aggtatgggg tatataaaat taggccaacc
     3121 tacaccaact ttaagtggtg gtgaatccca aagaatcaaa cttgcaaaag aaattggtaa
     3181 aaaacgaaat ggtaatatcc tttatgtatt ggatgagcca actaccggtc taagtctata
     3241 cgatacttct aagttgattc aattattaga tgaattggtt gaaaatggaa attctgtaat
     3301 tgttgttgaa catgatatag atgtattaaa tgtttgtgac tggattgttg agcttggtcc
     3361 agaaggtggt gacaaaggag gttatataat tgcagaaggt tctccaaaaa ctctaaaaga
     3421 aaatccaaaa tctataacag ggaggtatct ttaatgaata aaaacaattg cttagattta
     3481 atatttgaaa aagtaaatcc ttcaagtgtt ggtatgagtt cagaaaagct ctcacaactt
     3541 gaacctttaa taatctcaca atatagtaat ataaatggaa taattattgc aagaaaagga
     3601 aaagttgtct ttgaaaagta ctataatgga tataatgctg atgatatttt tcatgttgca
     3661 tcagttacaa aaagtattat atcagcctta ataggtattg caatagataa aggatatata
     3721 aaagatgttg accaaaaggt tgttgatttt ttccctgaat atacttgtag tccttctgaa
     3781 atatgtaaaa aagcagtaac tatacgtcac ctacttacaa tgactgcacc ataccctttt
     3841 aaaaatatga ctgagtcttt agaaagactt tgcaaacaaa aaaattggat aaaatatacc
     3901 ttgaatcaac ttggtcaaaa aggcgaaatt ggtactttca aatattcttc agctggaacg
     3961 cacctacttt cagctattat aacaaaaact actggaaaat ctgctcgtga atttgctaat
     4021 gaatacttat ttaaacctat tggtatgaaa gttataccag attatgatat gaaagatttt
     4081 ggttttgaag atttctttgg aaaaaatctt aagggttggg caaaagatag acagggttat
     4141 tctataggtg gctggggact tacattgagt gctagagata tgttgcgatt tggttttctg
     4201 tatttaaatg atggtatttg gaataataaa cgtattatat ctaaatcgtg gattgaagaa
     4261 tcggttaaaa tgaatagtaa tgaatacggt tatctgtggt ggctatgtaa aaaagatggt
     4321 gttttgtggt attcagcaat gggaactgga ggaaatgcta tttgttgtat tccacaatat
     4381 gacttagtaa tagccatacc atctaaaata gtgtttaaac ctcgtgatag atgggagttt
     4441 attgaaaaat atatacttac ttctataata gattaattac tctttcatag attaattact
     4501 ctttcataaa ttaattactc tttcataaac taattactct tagataacca aaccctccat
     4561 tataacacca gacttttgtt tggtgttatt atgactaata ctatatatct aaaaggagtt
     4621 gaatactaat ggacacttac acgacatcag aggtagcaga tttaattggt gtacatccta
     4681 atacagtacg tttatatgaa gaactaaact taatacctaa acctaagcgt aataccaatg
     4741 gttatcgtat ctttaatgat tttcatatag aacaatttag atttgctcgt acagccttga
     4801 aaattgaagt ggtacaaagg ggattaagaa aagatgccct tgagattgta agaacatcag
     4861 cgaataggga ctttgataag gctattagtc ttgtaaatca atacttgcaa caattagaaa
     4921 tagaaaaatc taatgccgag gaagcactta ctattgttgc acagatttta aatggaaata
     4981 ctccagaaac aaataaactc tttttaacta gaaaagaaac tgcagaacac ttaaacgtta
     5041 caatagatac tttaagaaat tgggaaatga atggtttgtt aaaagtaaaa agaaaacaaa
     5101 atggttatcg tgtatatact gatgaagata taaaacgact taaaattata agcgcacttc
     5161 gttgtgcaaa ctattctctt tcagctattt tacgaatgtt aaatgcttta tcttctaatc
     5221 ctaatactaa tattaaaact gtccttgaca ctccaaaaga tgatgacgaa attatctcag
     5281 tgtgtgacaa actgataact tcattgaaag aagctgaaca taatgcaaaa accatgaaat
     5341 ctcatcttca aattatgaaa aaacaatttt cctagccctc cactctaact ccagactttg
     5401 atttgtgtgt tatccttaag agcaagaaaa attcaacaca caaatcaaag gaggaatagg
     5461 atggaaaata aaaatactaa ttctacatgg aagaaaaaag tcattctttt tttagcaagt
     5521 caatatattt ccttatttgg ctcatcactt gtacaaatgg caatgatatg gtatgtcaca
     5581 cttcagacat catcaggtgt atgggtaaca gttttaactg tatgctcctt tatacctcaa
     5641 atgataattt ctttttttgc tggtgtatgg gcagactact acaatcgaaa ggtactcata
     5701 attgtatcag attcaacaat tgctatatct acattagttc taattctatt tatgatgtca
     5761 tcaaatggca caacaagtga attaatagcc atatcagtaa tatctgttat acgttcacta
     5821 ggaagtggtg tacaaacacc tgctgtaaac gcaatgattc ctcaacttgt accagaagaa
     5881 catttaatgc gttttaatgg tataaatagt agtctacagt ctatagtaca atttatagtt
     5941 cctattgtag ctgctgcaat acttggattt ggtaataaaa taaatcaagt tcttttaatt
     6001 gatgtattta ctgcaattat tgctattgta ttattaatat tcatcaaaat tcctaaacat
     6061 gatataccaa aacaaactga aaaagtctca ttttttgcag atatggtatc agggataaag
     6121 tactgttttt cagataagtt cattagaaaa gtgcttttta cttatggagc attcatgctt
     6181 ctttgtgttc catcaggatt tcttatagga cttcttattg aacgaacatt tggcaagggt
     6241 tatatatacc tgtctattat tgaaatgatt ggatttttag gtatggtttt aggtggattg
     6301 attataggag tttttggtgg ttttaaaaat agaaataaga ccttttttct agcaatatta
     6361 tcctatgcta ttttttcaat tattctaggt cttgtaacac aagcttggca attttttata
     6421 tttatgtttt ttacgaattt ttcaattcca atcattcaag catcagctat gactatgttg
     6481 caagagcatg tagcaccaca aatgctaggt agagttctta gcttacctat tattatatat
     6541 actggtttta ttcctcttgg tatgatgatt ttcggaccaa tggcagatgt aatttctatt
     6601 aataatttaa taatcatagc agggattata cttttatttt ttgctttggc tatcccctat
     6661 tcaaaacaat tttacaagca aggaatatct aagtcaaatg aatccgagta atatatttaa
     6721 atagtttaac tcgatttatc tggttaaaaa tgaaggggct taaaatggac tttttaattt
     6781 atgaagaaaa ttttttatat gaaatcaaat atattttatc atttcaacat tgaaaaatag
     6841 gtttatcttt att
//
LOCUS       DAEMIK010000181         6829 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEMIK010000181 DAEMIK010000000
VERSION     DAEMIK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.184"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..1230
                     /locus_tag="KPK87_003406"
     CDS             1..1230
                     /locus_tag="KPK87_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2539518.1"
                     /translation="MFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEIKEIDEYPMI
                     SILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILENIKDRYNNYN
                     FTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLVQTIVMNDEL
                     GAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTNFILRRSIIE
                     EIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQRTRWAKGNI
                     YVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISKLVEISIPIN
                     FFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFVAIKGMIEYL
                     KDIIFKREVKWYKTERF"
     gene            1253..3334
                     /locus_tag="KPK87_003407"
     CDS             1253..3334
                     /locus_tag="KPK87_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG2539519.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSINEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            3371..4456
                     /locus_tag="KPK87_003408"
     CDS             3371..4456
                     /locus_tag="KPK87_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2539520.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKVVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            4510..5634
                     /gene="wecB"
                     /locus_tag="KPK87_003409"
     CDS             4510..5634
                     /gene="wecB"
                     /locus_tag="KPK87_003409"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG2539521.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            5712..6686
                     /locus_tag="KPK87_003410"
     CDS             5712..6686
                     /locus_tag="KPK87_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2539522.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         2847 a          643 c         1135 g         2204 t
ORIGIN      
        1 ttgttcgtat ttagtctgtt tagtatatgg tctttattgc ttataaatat tattttagct
       61 atgggtggat atatatttta ttttaaaaac tttgataaag aaataaaaga aattgatgaa
      121 tatcctatga tatcaatact tgttcctgct cataatgaag ctaaagtgat aggtagaact
      181 gtggagtctt tgctattact taattatcct aagagtaaga tggaacttat tgttataaat
      241 gacaattctt ctgataatag taaagaaatt ttagaaaata taaaggatag gtacaacaat
      301 tataatttca ctataataaa tacagatagt ttaacaggtg gaaaaggaaa gtctaatgca
      361 ttaaatattg gatatacgat ttcaaaagga gattttatag ctgtttatga tgcagataac
      421 acaccagata aaaatgcact tagatatcta gtacaaacta tagtaatgaa tgatgaatta
      481 ggtgctgtta ttgggaaatt taggactcga aataaaaata aaaacctatt aaccaaattt
      541 ataaacatag aaacattaag ctttcaatgg atgtcacaag ctggtagatg gcaacttttt
      601 aatctatgta caataccagg aactaacttt atattgagga gaagtatcat agaagaaatt
      661 ggaggatggg atagtaaagc aattgcagaa gatacagaaa taagttttag gatatataaa
      721 ttgggataca agataaagct tgtaccacaa tctataacat gggaacaaga gccagaaact
      781 gttaaggtgt ggataaagca acgtactaga tgggcaaaag ggaatatata tgtattaatg
      841 aaatatataa aaaatatatt taaacaggga agaaataaga ttgtgtttga tatagcttac
      901 tttttttctg tatattttct atttttaaca tctgtaatta tatccgatat tttatttgtt
      961 ttaagtatat ctaaactagt agaaatttct attccaatta acttttttct aatatggata
     1021 ctatcttatc ttttatttat tatagaagtg agtataagcc ttactataga aaaaggtgaa
     1081 gcaacaattg aaaatatatt catagttgca ataatgtatt ttacatactc tcaactatgg
     1141 ctgtttgtag ctataaaagg aatgatagag tatctaaaag atataatttt taaaagagag
     1201 gttaaatggt ataaaacaga aagattttga ttcatgtaca ggaggaatta acatgaaaaa
     1261 atttattata tcaattatat ctttagtatt gtttttttca aatatatcac ttatatacaa
     1321 agtaaatgct gatgagacta aagttaaaaa ttataagttt gaaagagata taactattga
     1381 tggtgttata ggaagtaata gtactttttt tgaagtaaat aaaaattggg atattgaaga
     1441 agttttattg catcttaatt ttagtaagag ccaaatatta aatggagatg tttcaagttt
     1501 aactgtattg ataaataatg taccaataaa atctattaaa ttaaatgcta agacaaatta
     1561 taaaaataca ttagaagtat tagttccaaa agattatata attcaaggat ataatgaaat
     1621 aaaaataaaa acatacaaaa caatatctga taaaatttgc caagatgatt ccaatactgg
     1681 gaactggatg gttattcaca aagaatcata tatatcaatt agatataagc agaaaaaggt
     1741 ggaaaactca ataaatgaat atccgtatcc atatgcagaa attgaaaata atcataaatt
     1801 agatacaact atagtagtac cagataatat gactagagga gagactacag cagtgtttaa
     1861 tttggcatca gcatttggaa aaataacaaa aaatgatgat ttaaagttag atgttaaatt
     1921 atattcagaa atgaaaaatt ggtcagatga taatattata tatattggta aacctgaaaa
     1981 tacagcagaa gaaattttgg atattttaag tataaaggaa caaaccctat tatcttcaaa
     2041 ctgtataatc aagcaagtag attcaccata caataagaat aagaagatga tggtggtaat
     2101 tggtagtaat gaagatgatt taataaaggc atcaaattta ttaatagaaa atagactttc
     2161 taaccaagtt ttatcatcat cagtacttgt aaacaaagag actaatataa aaattaatag
     2221 ggaacaaaaa ttaaatttag gtcatttaac attaaaagac ttaggatatt ctgacttttt
     2281 gttggaagga gcatttaatc aacaagcttt gtttgatgtc aaaataccaa cgggtaaggt
     2341 attagatgat ggttcaaaga ttatcttaaa tttaagatat tctgacaatt tagattttga
     2401 aaaatcttta gttacagtgt ctataaatga tgttattgtg ggaagtaaaa aattggatag
     2461 gtcacattct aataatgata agttagaatt aaaaatacct aaagatatag ataataaaaa
     2521 ctattatcaa gttaaattga catttaattt gagtataaaa aattcgaatt gtgttactag
     2581 agagagtaat aatccttggg catatgtatc aaataattct tatttggcgt tatctacgaa
     2641 agaaaatgaa accttatctt ttgaaaatta tccgtatcca tttgttagag atgatgaatt
     2701 taatgattta actgtgataa tgccagatta ttcagggtca caagctatga cctggatgtt
     2761 tagattaggt gttactttag gtgcgaatat aaattcacat aatggaaata taaatgtaat
     2821 tagaggaaaa gaatttagtg acaagtataa ggatactaat attgtagttt ttggagtgcc
     2881 acataataat tctgttataa agatgttaaa taataattta aatataaagt tcgataaaaa
     2941 ttactccaat tttatatcaa atgataagat aagctttatt gatgattatg gaaaaaatat
     3001 ttctaccatt caattaataa aatcaccata taataatcaa aaaaatatca tggtaattag
     3061 ttcaataaat gaaaaaaact tatatttagg aatggattat ttgttgaata aaagtaaagt
     3121 taatgattta aaaggagata cattgataat tgatgaatac ggagaggtag aagatttagc
     3181 ttataattta aaatctaaga aagaagtaaa agattcaagt tggaatatga gtataaataa
     3241 gacaactaaa gtatttttaa tgatttcttt tattactata attgtagtca tgatattatc
     3301 aatgctatat ataaaaaaat ataaaagaag ataatatgat aaatttatta ttttaaaaga
     3361 ggtatataat ttgaggaaat atatgaataa atttaaatta aaaagaagta ttttaatctc
     3421 attaataatc ttaattttta tatttatagt ttactttgta agtattgagt taaaagatag
     3481 gagtctagta aataatcaat ataaaggtaa gattaaatca gccaatttat ctgtagatta
     3541 tgaaataaat caagttatga aggatataga taaattggga ttgaatacag taaatgtacc
     3601 tataattata aatgtagagt ccattaattc tgatattatg tcaatagata ataatagtaa
     3661 agaaaaggca attaagttaa taaaaaaatt aaataaaaaa ggtatttcaa caatattaga
     3721 ggcatatcca tggattgaaa atggaaaatt gtatgagact gactggaacc ctataaataa
     3781 aaaaagattt ttttatactt ggcaaaatgc catattaaat gaacttataa ttgatgtagc
     3841 aaatccactg gatgtaaatg ttttaaatat tggttctaat tttgtacatt tggaagaata
     3901 tcaacaaaat tggggtgaaa taatagattt tgtacaatcc aaatttgacg gtcttgtaac
     3961 gtacagaact aattggtggt atacaaaaaa agatgattta agtagtaagt tgttttatga
     4021 acaaaagttg aacaataatt tttttgataa gttagatttt atatctatag cagcatattt
     4081 tgagttaagc aataaacctg ttaatacagt ggatgaatta atttctgcct tacactcatc
     4141 tactgtaaac aatagaggac aaaatataaa acaagaaata tataaccttt ataaagctca
     4201 tagaaaacct atattttttg gagaattagg tttttcaaat agagaaagtg caagttctca
     4261 gccgtggaat catactcctt caaaagttgt aaatggcgaa gaacaagcaa ggtgttttga
     4321 ggcatataag aaagtttttg aaaatgaaga ctggataaat ggtttttctg tgttttgtgt
     4381 aggtaaaata gatgacgaaa aaaattttta cccatcaaaa gagagtataa aagttattaa
     4441 gtcttggtat gaataatttt gaaagtgtag aatttatata gattaaatag atgattagga
     4501 gagatataaa tgaatgacat caaggttatg actgtatttg gaactagacc tgaagctatc
     4561 aaagtagcac ctttaataaa ggaacttgaa aaaagagaga atataaaaag tattgtttgc
     4621 gttacagctc aacatagaga aatgcttgac caagttatag aaacctttaa tataaatgta
     4681 gactatgatt tggatataat ggaaaaagga caaagcctga atgatataac atgtaagatt
     4741 ttaaataaac ttcccttgat tttaaacaag gaaaatccaa atataatatt ggtacatgga
     4801 gatacaacca caactctagc tacaagtttg actgcatttt ataataaaac tcttgttggt
     4861 catatagaag ctgggcttag aacctatgat aaatattctc cttttccaga ggagttaaat
     4921 agacaactta caggtattat tgctgatatg cactttgctc ctactaattt ggctagaaaa
     4981 aacttgattt ctgaaggaaa acctaataat aatatatttg taactggtaa tactgctatt
     5041 gatgctttaa agatgacaat aaaagaaaac tataatcatc ctataattga tgagattggt
     5101 aacgatagga tgattttgtt aacctctcac agaagagaaa atttgggtaa acctatgaaa
     5161 aatatattca gagctataaa gcgaatagta gatgactttg aagatgtaca gatagtatat
     5221 cccatacatt taaatcctaa aatacgaact atagctgatg agatatttgg aaaatttcct
     5281 gaaaaaattc atattataga gcctttagat gtagctgatt ttcataactt tttaaataaa
     5341 tcttatatga taatgactga tagtggagga atacaggaag aagctccatc attaggtaag
     5401 ccagttcttg tgttgcgtga taaaacagaa agaacagaag gaattgaagc aaagacactt
     5461 aaattagtag gtactaatga agatagaata tataattcag tgagcgattt attaataaat
     5521 aaagataact atgttcagat gagtaaagca tctaatccat atggagatgg aaatgcaagc
     5581 aaatatatag tggatataat aattaaaaaa tttaattgca aatatttaaa ttagataagg
     5641 gctaaaacat taactaattg atataagatt tatgataata ttaaggaata caatttttaa
     5701 gggggagtaa tatggctaga aaattaataa aaaatttggg taaaagtaaa agtgtaaaga
     5761 gagtgaaact tttatttaag aagattttta ttactgtatt catagtagca agtatagttg
     5821 ctatttttaa tataacgaaa tattttgagg aattatacaa agtaagggat ttaaaaagta
     5881 ctaaaattga atactatatg gatgtggctg atgaagcagg agatggaaag gtccaattaa
     5941 gttggaaggc cttacttgct atagacatgg taatacatga tgaagattta agtaatataa
     6001 aaaagaaaga cacattggat ataggggaaa agtttatagt agaagataaa aatgataaag
     6061 gcgaaaaggt gtataaggta aaaaagttta ataaggtatt aagcgaattg aaatttgact
     6121 cttctcaaaa aagtagagca agaaaataca tgaaagattt agaatacaca taccttggaa
     6181 ataaacaatt agatagtagt gatgaaaaaa ttaaatttat aaagaagtta gaagactcag
     6241 ctataagaga atatattgat tatggaatat tgccctctat aacaattgga caagctatat
     6301 tagaatctgg ttggggaaat tctaaactta caaaacagag taataattta tttggtataa
     6361 aagcagataa agcatggaaa ggaaaaagtg tagaaatttc aacttcagag cattataatg
     6421 aaaaaattgt agctagtttt aggtcgtata attcattaca agattctgtc aaagaccaca
     6481 gtttattttt aattaataat aaaagatata gaaaacatgg gttgtttgag gcaaaagatt
     6541 atattagtca agctcaatca ttagaaaatg caggatatag tacagcagaa gataaaaaag
     6601 gaaatcgtat atatgcagag ctattaatag atgttattag gagttataat ttgcaattga
     6661 tagataataa agttgagaca aaataattgt taactttata tgaggagaaa acaagataag
     6721 attcttttct acaaaaagaa tagaaaaatt gtaaaaaaaa ctaaaaaaat gtattgactt
     6781 gttatttaaa aggtgatatt attattactg tactcgaaag agaacaaaa
//
LOCUS       DAEMIK010000182         6797 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEMIK010000182 DAEMIK010000000
VERSION     DAEMIK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.185"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(302..1198)
                     /locus_tag="KPK87_003411"
     CDS             complement(302..1198)
                     /locus_tag="KPK87_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2539523.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFFFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1339..2766
                     /locus_tag="KPK87_003412"
     CDS             1339..2766
                     /locus_tag="KPK87_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2539524.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2962..5556)
                     /gene="clpB"
                     /locus_tag="KPK87_003413"
     CDS             complement(2962..5556)
                     /gene="clpB"
                     /locus_tag="KPK87_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG2539525.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5593..6189)
                     /locus_tag="KPK87_003414"
     CDS             complement(5593..6189)
                     /locus_tag="KPK87_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539526.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6206..6610)
                     /locus_tag="KPK87_003415"
     CDS             complement(6206..6610)
                     /locus_tag="KPK87_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG2539527.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2177 a         1097 c          712 g         2811 t
ORIGIN      
        1 ttagctgttt acttgcttat ttaatagttt tatttctttg attaaatatc tttcagaatc
       61 taagccagat gtctcaaaaa aactgattta taatataatc attttttctg ctattcttat
      121 ttttccaatt ttagtttcat attaaaatat acttttcata gacactacat tctttcatac
      181 tcattttcac atttaactat acaagtagtc ttactcattt atgtactata ctttattaat
      241 atttattaag aaaaactcaa agatttaaaa atatatattt tctataaagt atatttatga
      301 attatttcat atcttctttt aaaataacat tactaacttc tttatgttca tttctatact
      361 tagttggaga tatattataa ttccttttaa ataagcgact aaaataattt atgctatcaa
      421 agccacatct catagcaatt tctgtcatat ttaaatctgt ttcctttaaa taatatatag
      481 ctttatcaag cctgattcca tttataaaat cgattgtagt cttacctgtt atttgcttaa
      541 atgttctaca aaagtgatgt gcactcaagt tcacatgact agcaagttct tttaatgtaa
      601 ttttatccat atatttttct tctatcaaat caaagataca ttcaaatttt tctaaagaat
      661 actttctttt tataaattca tcttcagaat atattttatc aatatgactt cttaataaga
      721 gtactattaa ttgatagata ttagctttta tagataactc atatccaact tctttattgt
      781 gatattcatc aagtatagtt gtaatacatt ttaaaatcag agaatcattt tgaattaaat
      841 tattaaatac aatatgattt aaagccaatg gtgctaagta cttggtttgt aacaaatcaa
      901 cttgattact aaaaaagaat gtgggttcaa tacgaatact atagaatttt aaattatctg
      961 ataagctttc taaataatgt aattcattac tgtttgctat tacaatattt ccaggcacta
     1021 ccttaaaatt atccaatcca cattcaagat atgctatacc atcaataaaa taatatattt
     1081 gtagatgctc atgccaatgt cttttaaagt ttgctccttt tttaatgtca tcctttaaaa
     1141 aaatatctat aaagttttct tgacctttcc tgttaaaata tgtattttta tccttcatta
     1201 ttcccccctc atcaaaatag tgctaattat aagcaatata gttattgtat tcattatttc
     1261 atatcgctat tatataaaat gtaactatca ttgtaaacat taacataatg cagtcattat
     1321 aataaggagg taataaaaat gacaaactct tttaaaaaag atgataatag aaaaaaaatc
     1381 acagttttat ttgtagtcat tgccatgact tttatggcaa cattagattc tagtattata
     1441 aatgttgctc tcccagttct tgcaagcaaa ctaaatgtat ctctagcatc tattgaatgg
     1501 gtaatagcaa gttactcaat tattatttgt tcaacactat tattttttgg aagacttgga
     1561 gatattattg gtaaatcaag agtatttcaa gtaggtacta ttctctttac atcagcttca
     1621 ttactatgtg gattaagtaa ttctttaact ttacttatag tttgccgctt tattcaagga
     1681 ataggcgctt ctgcctacat ggcaaacaat catggaatta tcacagaact atttccaaaa
     1741 gaaagcagag gaaaagccct agggatttta gttactgcag ttgctattgg aaatatggtt
     1801 ggaccatctg tgggaggatt tattttgtca atatttgatt ggaatgttat attttttata
     1861 aatattccaa tcggtcttat tgtaatcttt ttaaatacaa aatttcttcc taatagtaaa
     1921 aaatcttcag aaaatatgga taaaactggt gctatacttc aatttttagg aacaacatta
     1981 ttttttagtg ctctaattag tgctcaacaa acaggattac taaatccata tatattaatt
     2041 gcactattat tatcaattat ttttattata ttatttttga tattagaaaa aaaacatcct
     2101 caaccgcttc ttgaccttga aatttttaga aattttaaat tttcattgaa tttaatttgt
     2161 gctcttacat cattcatttg tattgcatca tcaagtattt taattccatt ttatttacaa
     2221 agtactatga aacttccccc tattcaagca ggtttattta tgatactatc acctttgatt
     2281 ttagccattt tttcacctat atttgggaat atatcagata aaattaagtc agaaaaaatt
     2341 attttaattg gtcttctcgt tatgagtttt ggattctttc taatgtcaag attgaaagaa
     2401 agttctgccc ttattttatt tgtaatttat atatcgataa tatcaatagg acaagctata
     2461 tttcaacctg ctaacaatgc tttgataatg tcaagctgtt ctagaagtaa gctaggagtt
     2521 gtaggaagca taaattccct tgtaagaaat ttaggacaag ttataggtat tactatttct
     2581 acaacacttt tatataattt tatgagtatt aaagctggat atagggtcaa tgattatgtg
     2641 attaataatg ataaaatatt tgtatttgga atgagaaatg tttatattat tgttacactt
     2701 gtttgcttaa taggtgcaat attaattggc ttttatttat ttaaatataa taaaaatgaa
     2761 caatagtttt tataattata ttcaaattat gattatatta taagaaattt actaaaatac
     2821 ttcctttgag ttaaatttaa tttggacata ttgacttaaa aatgttttat taatttgata
     2881 aaatatttct tttcctatag tttataaata aaaatcagct gagcaaaata tttagtatat
     2941 tatgctcaac tgattctttt attatttaac taaaacttca atattttcat cttttccatc
     3001 tataactata gtacatccat tatacacatc tccagcaata agtttctttg caattatagt
     3061 ttctaatgta tttcctatat atctcttaag aggtcttgct ccatatacag ggtcatatcc
     3121 ttctctcacc ataacatctt ttgctgaatt tgtaacttca atagtaatat ctttttcttt
     3181 taatctattt tttaaatctt tcataaatat atctataatt ttcttgatat tttcttggtc
     3241 taaaggctta aacattataa tatcatctac tctgtttaaa aactcaggtt taaatctatg
     3301 cttcatttca ttcattacta aattgttagt agtctctgtt atatttcctc ctgcttctaa
     3361 tagataatta cttcctatat tagaagtcat tattataaga gtatttttaa agtctactgt
     3421 cttaccttta ttatcagtaa gtctaccatc atctaatatc tgtaagaaca tgttaaatac
     3481 atcctcatgt gctttttcta tctcatcaaa tagtattaca gaataaggag cacgtctaac
     3541 tgcttctgta agttgaccac cctcttcata tcccacgtac cctggaggag gtccaactag
     3601 tcttgataca gcatgttttt ccatgtattc agacatatct attcttatta tattatcttc
     3661 actatcgaat aaatttctag caagagtttt tgcaagttca gttttaccaa cacctgtagg
     3721 gcctaagaaa ataaatgaac caattggttt tctttcatct tttaatcctg ctcttgcacg
     3781 tattactgca tttgaaactg ctgtaactgc ttcatcttgt ccaataactc ttttatgaag
     3841 ttcatcttct agtttaagta atttttctct ttctccttca actagtttag ttactggtat
     3901 acctgtccac tttgatacaa tgctagatat ttcttcttca gtaacttctt cttttaaaag
     3961 agaattttca gaaccatcat tcatattttc ttcgtactgc tttatttgtt cttctaactt
     4021 aggtatttca ccatatttaa cctctgctgc tttgttgaaa tcatattctc tttcatactt
     4081 ttcagcttta cctttagctt cgtctaattg agcctttaag tttttaatat ctaatatttg
     4141 actcttttct ttttcatatt ttgctgtcat ttcatcattt ttagatttta actctgctat
     4201 ttctttttga atatcatcca atcttgattt acttttatca tcattttctt taagtaatgc
     4261 ttctctttca gtttctaatg taaataactt tcttctaaca acatctaatt ctgttggcaa
     4321 tgaatctatt tcactacgaa tcatagctcc agcttcatca attaaatcaa ttgctttatc
     4381 tggtaaaaat ctatcttgta tatatctatg agaaagtttt gctgctgcta caatagcatt
     4441 atcatgtatt cttactccat gatgtatctc aaatctttct tttaaacctc taagtataga
     4501 tattgtatct tcaacagtag gttcttctgc aattacaggc tgaaaacgtc tttcaagagc
     4561 cttatctttt tctatatact ttctatattc atcaaaagta gttgcaccaa tacagttcaa
     4621 ttcacctctt gcaagcatag gtttaataag attacctgca tccattgaac cttctgtttt
     4681 accagctcca acaatagtat gaatctcatc tataaacagt ataatttttc catcagaact
     4741 ttgaacttct tttaaaactg cctttaatct ttcttcaaat tcacctctat attttgcacc
     4801 agcaattaaa gctcccatat caagtgagaa tacaatctta tcttttaaac cttctgggac
     4861 atctcctctt actattctct ctgctaaccc ttctacaatt gcagttttac ctacacctgg
     4921 ctcaccaatc aatacaggat tattttttgt tcttcttgat aaaattctta taatccttct
     4981 tatttcttca tctctcccaa ttacagggtc taatttattt ttcttggcta aatctactaa
     5041 atttgttcca tatctagcaa gtgcatcata tgtgccttct gggtcttgtg tttctactct
     5101 ttgactacct ctcacttgtg ataaaatatt taaaaagtca ttcttattta tattatattg
     5161 ttttaatatt ttaccaacag ctgattttga ttctgtttcc atcattgcaa gcataacatg
     5221 ctctacactt atatatgaat ctttaaattc tttagaaata ctctctgcct ttataagcac
     5281 ctcatttatt tttctagtag ctgatactcc ttgtgagtca gcaccttctc catatacttt
     5341 tggaagctta tcaatttcaa agtttacagt atttctcaaa gaatcaattg atatattcat
     5401 tttttcgatt atatttggta tcaatccatc ctcttgatta ataagtgctg agaaaagatg
     5461 aattacatct acttgttggt tatgattttt tactgcttcg ttataagcct catttaaact
     5521 tttttgaact cttaaagtca ttttttctac gtccatataa atccctccat tttaatatta
     5581 tatccaaata aattaaattc tctcatttat aattattgat tttctaagct ctaaaagact
     5641 atcaagtaaa ttctcgtttt tttgtatatc gaaactcaat gttaaattaa atgttccatc
     5701 tgaattaaaa aaatcgaata cttcttttga ttttgctatt aaatacaagt cttcatctat
     5761 aagtcttttt tcacctatta ataaatactg acatttatct ataaaactct ttttaaaatc
     5821 aatagtgtat agattttcta aaatattata ttgataaata gcaatattat taaacacttt
     5881 taatatgttt gaatttacaa aattgtaaat ttgattattg ttatacagtg atatttgttc
     5941 gtatatatat tccctaaata aaggatgaat gttttgctta agttttaaag taacttcagc
     6001 tggtatttca aaataaagct tccagtactt agagttattt ttttctaaaa taaaagctac
     6061 tttgttcaaa ctggtagaac taatctcctt tagtgtacaa ttaaaattat agtgtctcaa
     6121 actagttttt tcattctcaa atacatagtc aaaccactgt agaacatttc tatcaagttt
     6181 taaactcata taaaaacctt cttccttatt tactaacctt attaggtctt tttatctgat
     6241 tatattctgc taataaactt tctttatcag ctctactacc tttatatgct atatgtggat
     6301 ttatcatata ctcaccattt ttcactcttt gtaaaaaatc catttttttc agctccgtaa
     6361 acaatgaaga taaagaagtt tttggatatt caaattcaca agataattca tataatgttt
     6421 ttctaatctt attattttta tccatagagt ttattaattt taatggaatt aaatccctct
     6481 tagtatacat tccttctact actttaatat aattcttaaa acaaacattc atatacttac
     6541 ttttattcat aactaaactt ttgctctctg tatattcctg atttaaatcc ttttttaatg
     6601 tcttatccaa attaaatatc tcttctcact ctccttatct ctaattgata atataaatat
     6661 attacaaaaa aatatgtatg tcaaaactga aaatgcaaaa agttcacctt ataagcaaac
     6721 ttttatttag ctataagcta aactttatta tttttttaat ttagtatttc caatttattg
     6781 gcaggatttt tgaacaa
//
LOCUS       DAEMIK010000183         6606 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEMIK010000183 DAEMIK010000000
VERSION     DAEMIK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.186"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..680
                     /locus_tag="KPK87_003416"
     CDS             <1..680
                     /locus_tag="KPK87_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539528.1"
                     /translation="IDIKNKSYKLLNRFNLGLQNGEQQNINEIKFSIGSKLYLEIVNN
                     KSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYM
                     VKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDD
                     KIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQE
                     KLFFVAN"
     gene            1046..2227
                     /locus_tag="KPK87_003417"
     CDS             1046..2227
                     /locus_tag="KPK87_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2539529.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(2369..3286)
                     /locus_tag="KPK87_003418"
     CDS             complement(2369..3286)
                     /locus_tag="KPK87_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539530.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            3539..4333
                     /locus_tag="KPK87_003419"
     CDS             3539..4333
                     /locus_tag="KPK87_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539531.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            4334..6394
                     /locus_tag="KPK87_003420"
     CDS             4334..6394
                     /locus_tag="KPK87_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2539532.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLSLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2572 a          704 c         1065 g         2265 t
ORIGIN      
        1 agatagatat aaaaaataag tcttataaat tattaaatag atttaattta ggattacaga
       61 atggagaaca acaaaatatc aatgaaatta aattttctat tggaagtaaa ttatacttag
      121 aaatagtaaa taataaaagt aaaaatggat atattgaagg acatgatagt tactctaact
      181 ttattatata tgatatagaa gataacactt ttaatataag taataaattt atggataatc
      241 taaacaaaga aaagaatgca aatccaaagg agattggctt attaagtaaa actaagctag
      301 attatatggt aaaaaataat aaattgaatc ttatagttac ggatatgagt aattccataa
      361 ctaatcttgt ttatgatatt aattctaatg agattaaatt agaagattat gagatacttg
      421 atttaaaagt agattataat gaagcatcat ctggagttag gaaagttaag ctactggatg
      481 ataagatata ttctatttca ggtattaaga acagtatatc aggagaatta aaaggattta
      541 aagggattgt tgatgtggtc ggaactaagc cagtaaaatt tcaagtattt gatatgaaac
      601 ttaaaaagaa tatttatgaa gctgagctaa taaatggaga tataagttta caagaaaagt
      661 tattttttgt tgcaaattaa gtgttagaat taattttaga actttaaact tttatatgta
      721 tcacagataa gtataagtaa gggagtgtct caaaataaac tttttagctc atgagacact
      781 ttttttatat gaaatcaaat atattttcat tgtttagatt tattttgata atgaaaaaaa
      841 gattatcttt attttaagaa agacatttat ttataattat atacaaaaat attttttaat
      901 gtttttgtgt ataattataa caatttaatt tacattaatg cacttaaatt tgaaatagaa
      961 tgcttatagt ggtaaaatta attattagaa tattagaaat aataaataaa taatacattg
     1021 tacttaaaag ggggagaagt ttaagatgaa caaaaaatat attaaagcag ggaatttaat
     1081 tgatggaacc ggaaatagta ttatgcaaaa taagggtata attattgatg gagatactat
     1141 agttgaaatt gaagatataa gagatgattt aaatggatat gatgtgtacg attacttaga
     1201 taaaactgtt atgccaggca ttatgaattg tcatgttcac ttgacaatgg agccagttgg
     1261 aaatccaaag atatattatg ataatgtatc agatgtagag cttgtagtaa atacgataaa
     1321 acaacttgat gcttatttgg attcaggagt aacatatatt agaagcttag gttgtcccaa
     1381 atatgtggat gtacaactta aaaatcttat agacaaaggg tctatagatg gtcctggtat
     1441 agttacttca ggtccagtta tatgtatgac aggagggcat ggatattatt ttggtattga
     1501 aagtgatgga gtggatgaat gtagaaaatc agccagaaca gttttaaaaa atggagtaga
     1561 ttgtgtaaag ataatggcaa ctggaggtgt aacaactgat ggagttgagc cagggtcacc
     1621 acaattaact ttggaggaaa tgaaggctgc tgtagatgaa gctataaaag caggtaaaat
     1681 aactgccaca catgcacaag gaagaactgg tataaaaaat gcagtgcttg caggaattac
     1741 ttctatagaa catggtgttt atttagatga tgaaataatt gatttgatgc ttgaaagagg
     1801 aacttactta gttccaactg tggctgcacc atattttatt ctaaatgcag gaaaagaaag
     1861 tggaataact gaagctactc ttagaaagtg tgagattgtt gccattccac acaaagagtc
     1921 attcctaaaa gcatataaaa aaggaattaa aattgcagtt ggttctgatg caggaacttc
     1981 ttataatgaa catggaaaaa catattatga aatgaaacta atgtcagatt atggtatgga
     2041 taatatggat ataatagttg ctgctacaaa aacagcatct gaacttttaa gaattgataa
     2101 aaattatgga actctagaaa aaggaaagaa agcagatgtt atagttgtaa atggaaatcc
     2161 agtagagaat atagacgtac ttgcagatgt gttagcagta tttaaacttg gtaaaaaagt
     2221 tagataaata gtataaaaat aaaaaagagt gtctaaagaa atttttaaat aattatagac
     2281 actctttttt attagtaata attaaatctt ttatacttta gaaaatagat tctattttat
     2341 atttagatat aactctttaa aatcattctt atgcaatata atctatagaa tctacacata
     2401 caaagtatat tcttgaatca ttagaatttc caagtactat aacatcattt ttcttaccta
     2461 gaacttcaac accagttaga accaaactac cagctactac agtaacttga ttatttatat
     2521 ttgacatata aagattatct aggatacctt tattacaaca gcaatcatca caatcgcagt
     2581 tgcaattaca ttctttgtaa tgttttttat caagatagta acctaaagat gctatgaaat
     2641 cttcataatc tgcaggtgta atatcaaatg caacagcttt aagattacac aatgatgcta
     2701 aatctacgtt tgctaatgga gtagatgcag caagtataaa atcaattata gcagcaagta
     2761 ttgcatctaa tatagcccca aggtctatcg taggaggaat aactgcatca ataagtgcaa
     2821 ttaattcagc tactcctggt atagttccta tagtattaat taatccctca agagttaaag
     2881 ggactggaat aggataagat actctaccag ctatatctat taaatcacag ttacaagcag
     2941 aaaatctttc aaaagtacca tcaagtccag ataaattatc ttttggagga gctgaaagat
     3001 ctatacctac caaattagca cctactataa agaaatctga gataaaagca aattgattaa
     3061 agtttacaaa aggtcttaga gcatcatatc ttagaagttc taaggcctct ctcatacttt
     3121 ttttacaaca acattgtttt attttaggtt ctttatattt atttggataa ttatagtatc
     3181 tatcagctac ttgataattt tcatagtatc tttcatttgt atttggataa tcttcctcat
     3241 attcttgtgt aaatctaaag tcctctctac atttattatt ttccactaaa tacactcctt
     3301 ttatatttta atttaaatgt atagataatt aagcttatct atatagtaat attatattta
     3361 taatgtaaaa aaatgttaca aaagatatta taaacgaata aaaaataata atttgtcaga
     3421 taaacttgta aatttttatg aataatccat tctaaaacaa aaaactgtgt ataaaaaagg
     3481 tttatatgtt atatttatat aaatataaca tatgaacgag aaaggagtgg tttgataaat
     3541 gattgaggta gataaattgt gtaagtcttt tacaagggtt gtaaaagatg ataaaaataa
     3601 aagttcaata agaaagctaa agaaagtaaa aactaaaaaa gaagaatttt ttgctgtaaa
     3661 taatgtttca tttaaagtta tggaaggtga aattgttgga atactaggtc caaatggtgc
     3721 aggaaagact acattactta gaatgttagg tggcatatta acaccaacat caggaagtat
     3781 aaatatttct ggatatgatt attcaataga tagaaattca gcaaaaaaag aaataggtta
     3841 tctctctgga aatacaaaac tatatggaag gctttctccg agagaacttc taacaacttt
     3901 tgcaagtcta tatgagatgt caaaagaaga tatagaagaa tcaattgaaa atattgtaaa
     3961 aataatggat atgagtgaat ttatagataa tagaattgaa aatctatcaa ctggtcagac
     4021 tcaaagaaca tctatagctc gttgtttaat acattctcca aaagtatata tttttgatga
     4081 gccaacacta ggtctggatg ttttaagtag tgcatctatt atagatttta tgaagaatga
     4141 aaaactaagg ggaaaaactg ttctttactc aactcattac atggaagaag cagaaacttt
     4201 atgtgacaag atttttatga ttcacaatgg gaaaataatt gcatcaggaa caccacagag
     4261 tttaaaagag gaatctggag taaataattt gagagatgta ttcataaaac ttgccagaaa
     4321 ggaggacatt taaatgagaa gtaagattgt aaaatacata tttaaaaaag aaatgttaga
     4381 catattgaga gataaaaaga ctttatttat gatgattgtt gtgccacttc ttttatatcc
     4441 tttacttatg ttattaatga ttcaagtaat gaatatgtct gcaagtagta tgaatagtaa
     4501 ggacattaat atagctttta ataataaacc taataaggtt ttagtatcca tgattgaaga
     4561 tgataattta gaaaaagata ctaaaaatac ttcaaatgat aaaggcaaaa taagagtgtt
     4621 agatgtagat aactataaaa agtcattaga agaaaataaa attgatgctt atataaagat
     4681 ggaagaaaaa aaatcatcta taaattatca aatatatata aattcttctc aagaaaattc
     4741 ttctaatgca ctaaaaagaa ttgaaactgt tttggataaa tataaaagat ttaccataga
     4801 aaaaacttta tctgaaaaag gattagatgt acaagctact ttagaaccaa taacatatag
     4861 cactgtagat gttgcaaaga cggaagaagt ggcaggatac tttttaggtc agatacttcc
     4921 atttatatta ataataggag ttttactagg ttcaatatat ccagcaatag atgttatggc
     4981 tggagaaaag gaaagaggta cattagaaac attatttaca ctcccaatat caaacttaga
     5041 actggttatg ggaaaatata tggcagtttc tcttagtgca atagtaacag caattttaaa
     5101 cattttatct atgggactga ctatggcatt tattttagtt agtggaggta ttagtagcca
     5161 atttggcttt ggaaacttta attatggaga cttagtctta ccaattataa ctactttaat
     5221 ttgcatatgt cttttttcaa tggtagtttc agcaataagt atgtgtgtat gttcattagc
     5281 aaaaagtttt aaagatgctc aaaactacat aacacctctt atgttaatta ttatgatacc
     5341 atcatatgtt tctatgatac caaatatagt attagataga gtaacagctg taatacctgt
     5401 agtaaatata tctttactta taaaaagtgt attatctttt aaaagcgatt taaatttaat
     5461 agccttagtt ttagcatcaa atgtagcctt tgttatattg gcaatcatac tactgtctaa
     5521 gatgttcaat tctgaggaaa tactttttgg taatagtaag agtttctctt tcttagagaa
     5581 aagaagtaat ataaaaaaag ggtctatacc aactgtaagt gatggtgtta ttttatatgc
     5641 agtaggatta gttctattga tttatgttgg ttctttagtt caaattaaat ttggtatggc
     5701 aggtattata atgacacaat tcatgataat ttctttatcg ttattatttg catactatat
     5761 taaagctgat tttaaagagg tatttagctt gaaagttccc aaattaaagc atgtgtttgg
     5821 gagcattgtt ctttggattg gtggatacat acttattatg attataacac agctaatttt
     5881 attcttattt cctcaaaata tggaagttgc tgaaagttta aataaggctc tttttatcaa
     5941 agatagtgtt ttactgaatt tactagcaat agctttatta ccagctatat gtgaagaaat
     6001 gttttttagg ggatttatat tttcttcatt tagtaaatca aaagataaaa ataagtctat
     6061 caagctagct ataatatgca gtggggtact atttggcatt atgcatatgg attttattag
     6121 aataattcca acatcaatat taggtattat atttgcatat agtgtgtata aatctggttc
     6181 aatatttgta tcaatgttat tgcatttttt aaataatagt gtagcagttg tgttaaatca
     6241 ttatacaact ggaaatataa ccaacttata taaatttgtt gaagttgatt tttcgaactt
     6301 aaatgtactt aaatttgcct taattcttct aataagtctt atattaatta tgttaggatt
     6361 aagagtttta gatagaaagt ctttaagaaa ttaagtataa agttattata tagattaaaa
     6421 tattaataag cactaaagta tatggatata tcttgtatat aaaaatactt tagtgctatt
     6481 ttatatattt taattatatg aattatttat cgtttcattt atgtaatgaa aatgttatat
     6541 aaatgaaaca ataaataatt tttattttag aaatttatat ttaaataatc aagttgaaag
     6601 tgagat
//
LOCUS       DAEMIK010000184         6591 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEMIK010000184 DAEMIK010000000
VERSION     DAEMIK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.187"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..183)
                     /locus_tag="KPK87_003421"
     CDS             complement(<1..183)
                     /locus_tag="KPK87_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539533.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLT"
     gene            complement(832..1239)
                     /locus_tag="KPK87_003422"
     CDS             complement(832..1239)
                     /locus_tag="KPK87_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539534.1"
                     /translation="MSKVAKAAFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1713..2597)
                     /locus_tag="KPK87_003423"
     CDS             complement(1713..2597)
                     /locus_tag="KPK87_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2539535.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2888..3364)
                     /locus_tag="KPK87_003424"
     CDS             complement(2888..3364)
                     /locus_tag="KPK87_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG2539536.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEDVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDENCGCGCNHHHEN"
     gene            3631..4806
                     /locus_tag="KPK87_003425"
     CDS             3631..4806
                     /locus_tag="KPK87_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG2539537.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4887..5690)
                     /gene="proC"
                     /locus_tag="KPK87_003426"
     CDS             complement(4887..5690)
                     /gene="proC"
                     /locus_tag="KPK87_003426"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG2539538.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAISSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5730..6314)
                     /locus_tag="KPK87_003427"
     CDS             complement(5730..6314)
                     /locus_tag="KPK87_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539539.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2166 a         1125 c          747 g         2553 t
ORIGIN      
        1 ggtcaaatca gaactacact gtttataagt aaaagtttct ttatcgtttt tataaacatc
       61 tattctttta gcaagatact tattatatac aaatctacaa catccaaaag ttttgttaat
      121 taactcttgt tgttttttat ttggatacat tctaaactta taagcttttt tcactgctat
      181 cacctcactt taattgtttt ttgatacact ttttaatgat tttatcacat tttatcacat
      241 ttaatatatt atataatgct gtggtaaaca atttattaat cacctataat aatgttaaca
      301 aatgtaatta tttaaaacaa ttatttagaa ggttttatat ttaattttaa ataacatatt
      361 taagaaccgt aagtcttaac ttttatcatt tttgaggttg tcgttcacat aagttcgcta
      421 ctacctatgc agttctctta tgaacttctt acactttcat gtaagcacag actatatctt
      481 atccttcagc attatccgtt aaggtctacc cacttccact accaatagct tgtagtgtac
      541 ttccctcaag aggaatagtc gttgaagttt ctcctattcg gagtttacct gctgattgcc
      601 cattttaaag tacttaggat ttaaccttat accatctaac caattttttc tactttcgta
      661 actttcacac ttaggtttat ttcatcctta tgttttagtt tgattagctt taggggtttc
      721 cagcaattca agtagtattg gataggttct atcctatctc tacatatagg tttccctata
      781 tgctgactat tttagctatt aactcatgac taaagtcacg agtgtgtgta gctatttttt
      841 aatcaatatt ttagtaaatt ttacggtaag taaaaacctc tctccttcaa atcctatagc
      901 aaatatactt acaagttgct ttgagaaaat aactcctaat atagctacaa ctatgcatat
      961 accaactatt atatttaaaa cattgtttaa aaactttagt gcttgctttt caccttgttt
     1021 actacttata tcttgataca ttggaataaa agtagtaact atagcagtac ctattgctgc
     1081 aaatataata tttggtatat tcatagctgt taaataactc tctgtatata agcctgtacc
     1141 ataaattgat gataatacta attctctacc cattccaaga atcttagaga ttatagtaac
     1201 tatcatcaaa taaaaagctg ctttagctac cttgctcatt ttatcactcc acttctaaca
     1261 tacctctttt taatcaagta aaatttagct cattagatat aattctatca actaatttgt
     1321 cttgcttagc cctatcaaaa tataattaac acctatttct taatatatca caaactacat
     1381 tattcttcat atttttttat ttctccaata agttttacat ctttcctata taaattttat
     1441 agcttcaatt atattcaaag aaatggagtt gctacccgat aactttaaaa gtaaaaaaca
     1501 gattttatta tctttttaaa ctatattcta aaatattttg tccatattga gaaattttac
     1561 taaactatta tgtactttat taaataaata aaataacagc acacctataa aatacaagtg
     1621 tgctgttcat aaacagattt tataaaaaaa taattcctaa gtagagatta acatgctttc
     1681 tcatactctc atatataatg tgtctaaaaa tattaataaa aatgctttaa ttgttttaac
     1741 ttacttggat gtcttaattt tcttattgct ttagcttcta tctgtcttat tcgttctctg
     1801 gtaactccaa atacctttcc aatctgttct agtgttttag ggtcatcatc atcaattcca
     1861 aatctcattc ttaaaacttg ctgttcttgc tctccaagac ctgtaagtag ttcttctatc
     1921 ttttctttta aattatgctg ttcaacttcg tgtattacaa catctttaaa atcagcatct
     1981 gatggtataa attcaactaa actactatcc tcatcttctt ttaatggagt atccaaagat
     2041 acaacatttt tatctcttct taataattcc aacactttgt caacttctaa gccacatgca
     2101 tctgcaatct catctatagt tggctctctt aataatacat ttaaaagttc ttgtttcttt
     2161 ttctttacaa aatttattct ctgatgaaga tgtataggta tcctaatagt attttcgcaa
     2221 tcgtcgatat atcttgttat actttgtttt atccaccaag ttgcataagt gctaaatttg
     2281 tatccttttt tataatcgta cttttctaca gcttttataa gtcctatatt tcctgcttga
     2341 attaaatcca acatatcaat actatctctt ttatatctct ttgctatgct cactaccaac
     2401 ctataatttg aatttataaa tttttctttt gctactgaag atgagttgat tatttcctta
     2461 gccagctcta cctcttctcc tgctgacaac attttgtatt cctctatttc ctttaaatac
     2521 attttcatcg cattcgatac atttgatgaa cccacacaaa tataattttc ataattattt
     2581 tcttttttag tatccataat caaacccctt ctattcatag aatttgctaa aacaaaaata
     2641 aataatttaa acctacataa tctacaatta cataatatcc cattctttaa atgaattgtt
     2701 ttgtatatta tgtatacaaa tttataaaaa ctatacctat aatattattc tacttctaac
     2761 agcaatatcc tccttatata tataatttat tttattatat gttgttatat tcttagtttt
     2821 ttgaatgata tatgtctaaa aaaagactgt ttactaaaca gtcttttttt atatatatgc
     2881 tcaatattta attttcatga tgatgattac atccacatcc acaattttca tcatggtgat
     2941 gatgttcttc taaatctttt gcctgttgtt ccatttcatg aaactctttt accattatat
     3001 cataagttgg aagagctgcc tcagtatcgt attcttcccc tctccaatca gcgtacatca
     3061 tagcatctcc attagttaaa accaatctac ctgacatagt tatattatcc acgtcttcta
     3121 aaaattctat ttgtttttgt aaaacttctc cttctaactc cacatcttca tctaaacatt
     3181 gcactatcat tacaggtgca taataatctt cttcatcttt aacatttaca acaatatcta
     3241 aaattccttc tgcaccttct tcaaattctg ccaactcaac atttgtatca atcatatctt
     3301 gaggtactaa taaatcttct attaaatatt ttattactct atcttttact aattcttttg
     3361 acatttattt ttctccttcc gattctataa cattatcata atcatttcta tattatagta
     3421 tactacatta attgtttttt caatctctta caaaaattat ttttttatta tcttccttat
     3481 attccctttt ttattacaaa gttgtaatcg cattgattta aaaaatagtt tatatttagt
     3541 ataatagaaa tgtagcacat atttttattc aatatattaa taaaaaaata gattgaaaat
     3601 gtaatgtatg ttaaatctag gaggaatatt atgaagttta aaaaacaaat tagttctatt
     3661 gttgtgtcta ctatgcttgt acttgggagt atgaaccttt cttatgcaga cggtacaaat
     3721 gtagttacac ttggagctaa cttatcagag tctcaaaagc agcaaatgct taattacttt
     3781 ggagtaaaaa aagaccaagt cttagtttta gatgtaacaa atgcagaaga aagacagtat
     3841 ttacaaggtg ttgcaacaga gggtcaactt ggaaaagtta ctatctcttg ctcatacgtt
     3901 gaacctacta aaaaaggaaa tggaattaat gttaaaacag caaacttaac ttgggtaact
     3961 agttcaatga ttgcttcaac ccttacaaca gctggtcttg aagatgcaaa tgttattgct
     4021 gctgctccat atcctgtaag tggtactgga gcattgacag gaatcatgaa agcatttgag
     4081 gatgcttcag gaaagaaact tgatgaaact aagaaagaaa ttgcttctga agaattggta
     4141 gttacaggtg acttaggaga tgatataggg caagaaaaag caacaggtgt aatgaatgat
     4201 ataaaaacag aaattgtaaa gaatggtaca aaagatacca atcaaatagc aaatacaata
     4261 aataatgttg taaataatta caatattact attacaccag aacaaaaaga acaaattaaa
     4321 ggtgtaatga aaaaaatagc tgaccaaaat tacgattaca atagcatgaa aaatacacta
     4381 gataatgtat cagataatgt aaatgaacaa ttaaagaaat taggtgaaag tgttaagggt
     4441 tctggtatac tagatactat tggtggatgg tttagtggaa taggagactg gttctctggt
     4501 ttattctcag gcggagataa aaaagatttg ggaatcttaa ataacactaa tgatgagtct
     4561 cttggctctg atgctgtaat aaattctaca gaaggtgttc aaataaatcc acaaaataat
     4621 actaattcta acaatgatag tgatacaaat agctcaaaaa atgatacttc aagtaataat
     4681 gaaaataacg atagttctca aacaaatgaa aacaacacaa caaataattc agaaaatcaa
     4741 tcaaataata atgactcagt tggtttcttt gaaaaaataa aaaattggct ttcaggctta
     4801 ttttagaaat taaacaaata aaaagaattg cctaaaatgg aaattttcta taaaaggcaa
     4861 ttctctttat ttacaataaa tttttattac tcactcattt ttttagattt ttctatacat
     4921 gcacatattg cttttatcac agaagctcta aaaccttctt cttcaagtac tttaactgct
     4981 tctatagtag ttccaccagg tgaacatacc atatctttta attcaccagg atgttttcct
     5041 gtctcaagta ccatttttgc agaacccata acagcctgtg atgcaaactt atatgcttgt
     5101 ggtcttggca ttcctgcaat tactgctgca tctgctatag cttctataaa taaaaataca
     5161 tatgctggtg cagaaccact tgcaccaatt acagcttcta taagatactc tggtactacc
     5221 tcagtatttc caaaagaatt aaatacttct gtaactgctt ctaagtcttc tttgtttata
     5281 tttttattta tagaaagaga agatatggct tcatttacaa gagctggagt atttggcata
     5341 gttctaacta tttttttatc ttctcctata atagactcta tatcttttat agattttcct
     5401 gctgctattg taactataat tttattgtca tctataaagt cttttatctc ttccaaaata
     5461 tcatcatata tatctggttt aacagctaca attactatat cagaggttct agttacttcc
     5521 ttcgagtctt ttgtagtatt tattccaaaa tttgcatcta ttctatcgag agttccttga
     5581 ctatatgctg atgctataac catattaggt tcaactaatt ttgaatttac tattccccct
     5641 atcatagcgc ttgccatatt tcctgcacct atgaatccaa tttttttcac tttagtacct
     5701 ccttattgac taatagtaat cgttaaaatt taagcttctt taactgcatc agacttttca
     5761 aatggatttg gtatacttac taccaaaatt ttcactacct tattagtata gttgacccaa
     5821 ttatgattgt tcttttcact attatagtgt attgtgtctc ctggatacat ttctatttgt
     5881 tcatctccca aaaacactgt aagagtaccc tctaatacat acacaaattc ctcaccttca
     5941 tgtacgtaac aacaaatttc ctcactactc ttacttggaa gaatttcaat taatcttggt
     6001 agcattgttt tttcctttaa actagatgat aaatgataat gtataaatgt tgagttttct
     6061 acatcaaata cttctttttc ataacttctt aatacagctc ttttatgctc ttttggcttc
     6121 ataaaaaagt aagttaattc tacatctagt acacttgcta tttttcctaa ggaatctgtt
     6181 gccacacttg ttaatcctct ttcaagttgt gataaaaaac caattgacaa gtttgttttt
     6241 tcactcatat ccttcagcgt catttgcttt tgagttctga gttgctttat ttttgctcct
     6301 atgtccttat tcaaaaccat cacccctaaa taattttaat tttttcataa tataaataca
     6361 aaaatataaa tataggtact gaacctatct ttatttcatt taaaaaaatt tttataagta
     6421 ttactataat ttatcaatct ttttttgtct acatttttac gattatttac gatttatttt
     6481 tcacatatgt atatttaatc ttgccaacta aagatacatt ttttataagc atttactctt
     6541 tatataataa aacttaagtt gttcatgtag tcttcttctg gtttaattat t
//
LOCUS       DAEMIK010000185         6588 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEMIK010000185 DAEMIK010000000
VERSION     DAEMIK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.188"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..241)
                     /locus_tag="KPK87_003428"
     CDS             complement(<1..241)
                     /locus_tag="KPK87_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539540.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQEL"
     gene            complement(231..2222)
                     /locus_tag="KPK87_003429"
     CDS             complement(231..2222)
                     /locus_tag="KPK87_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539541.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(2345..4888)
                     /locus_tag="KPK87_003430"
     CDS             complement(2345..4888)
                     /locus_tag="KPK87_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG2539542.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEMEQNEL
                     KEIKQKEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(5166..5500)
                     /gene="rnpB"
                     /locus_tag="KPK87_003431"
     ncRNA           complement(5166..5500)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPK87_003431"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5632..6309
                     /gene="srtB"
                     /locus_tag="KPK87_003432"
     CDS             5632..6309
                     /gene="srtB"
                     /locus_tag="KPK87_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG2539543.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(6469..6543)
                     /locus_tag="KPK87_003433"
     tRNA            complement(6469..6543)
                     /locus_tag="KPK87_003433"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6509..6511),aa:Thr,seq:tgt)
BASE COUNT         2034 a         1102 c          605 g         2847 t
ORIGIN      
        1 ctagttcttg atatgtcata tcaacactga catcctcatc tatatccatt tctgatatta
       61 attctcctaa aatactattt ccatcagaat ctaaaccact atctatatct tcatctatat
      121 agttttcatt taaaatttca tctaaatcaa tctcataata aacttcatta tttttttcat
      181 cccctgagtc caatcctaag aactcatagt taaaagtcca aacattgtat ttacttgaca
      241 tactttattc ctcctgtgaa aaacatattt tcatctagtt tattggttcc ataatcaaca
      301 gagttatata ttttttcaat tatgtctttt cttctttctt ttgtactatt atttaatgtc
      361 aattgtttaa atacatatgt atttgttatt ctctcgaata atatttcttt tatactatca
      421 tcatcaatat ttctatagtt tttccacaat aactcaaata acttaaacat ttccctacta
      481 gtttcatcta cataataagg atacatattt tctactaaat ggtttaatct tgatatggct
      541 tgttttaaaa atacatcaag acttgcactt tcactttctt ttaacattct ataattgtat
      601 ttcttcttat cttctcctga aattacaaag atgatattgt ctaaactttt ttcaacttca
      661 tttaaataac tgcttatatt atatgataaa tcagacaata gatttttaat ctgcttaaac
      721 acttgtacat caagtttagt attatacttt tcaaacaaat ctatttcttg attttctaat
      781 attcttggca tatatgctga caataaatct aaattgtttc tttgttcttg tatttgctta
      841 taaagtgaat ttataacttc tattcttatt tcatcattta actctttttc taaaatctta
      901 tcaaattcag atttcatata gtaaatattt ccaaagaaat taaatattat catatcattc
      961 atagctttag accaattctt atatatcact tcaatagaat tttctttaag tagaggttgc
     1021 tttgcatata taaatacatc ttgcataagt ttttgaacca tacttttact taagtttaaa
     1081 agtacttttt gattattaaa ttttatttcc gtttctacaa aattagaatt ttcgttttca
     1141 agttctttat taattacaat gtcttttata tcactatttt ttatagatat gatttctttt
     1201 tgaaaagact ttagaatagt caagctatcg aaatcttctt gaataaaaaa tggagcatgt
     1261 tttaatgtat atcccactat ttcattttct atatctttta ggtcatctat ttgcataatt
     1321 tctctatttt tatcatttat atgtaggtta aatacatttt tattatcata taatattata
     1381 tcttctccta aatttaactt agaacaatta tctttcgctg actttacaat agtcttctta
     1441 gatttcttat ttataaaaat taattggtct tgatatttta ctaaaagcat gtttgtgttt
     1501 ttataatcaa atccatctat atttcctgat atcttgcaga atactatttc acaatcttta
     1561 ggaagatttc ctattttttg gttatcttta actctctcat ttaaatcaga tgggactaca
     1621 ctatctatat tgttattaaa tatagataca gaccttacta taatcccctc tctttccata
     1681 tgaaagtatc ccttcaatga cactctttta tcttctaaaa tgtatctttc tatatcatca
     1741 aaatttattt caaatctatt tttcccactg ataaatataa acttctcatt atctttatta
     1801 attccaccca tataaaactt attgtcatct aaacttaata tagtcatatt atcctttaga
     1861 tagaatataa attctctttg acttttgcag attaaatcat attcatccaa agttattttc
     1921 atagtcaaat ttgctgtaac atttttactt gaattttcac tttttatgta aaaaggcaag
     1981 ttatttccat agacaattat ttgagaatct tctaaaactg cctctaattt ttctttattg
     2041 tctgaaaaac tatatatata ttttacacct gtatcctcat taacttcatc tgatttgctg
     2101 attattatat tccctagatt tgttatatga agctttttat attcttttga gtatctactt
     2161 atgccactaa gctcaaaccc atctgaaagc tcatatatga ttttttctcc taatttcaac
     2221 ataccttcac cacctaaaca gaattataag tattttataa aggtgaataa aatgtctcaa
     2281 aacaaataat atttttttta atacttttta ttctgagaca tagtttctcc ttttttataa
     2341 tgctctaagc accttcaact atttcttcat aaagtttatc taaatgttta acatattcac
     2401 gatatccaga tttgttcttt aactcacttc ttaaatcttc atatttttgt ttaagttctt
     2461 cagcactcaa agaaggagct gattctttta attcttctaa cttataatca tagtacttat
     2521 catacatagc tatatatgta tcaatagact tcttagctag ttcattacat ttttcttgtg
     2581 cttctccata ttgagaataa tcacctattt ttattttata gtatccattt tcttgaatta
     2641 ttgttgaaga aataccttta gcctcatatt cttttgctgt ttcttttgct gttttttcaa
     2701 ctttaagtga acctgcataa acagtgtaat atacaccttt accatcttta tatgcttcca
     2761 tttcttttgc tttcttttca gcttgtgctt tttcttgagc ctctttttca gcttttgcct
     2821 tttcttctgc ttcttttttc tctttttcag cttgtgcttt tttctcttct tcagttaatt
     2881 tattgttagc ttgttgttga gttgatgact ttggaatatt ctctttatca tttttcttat
     2941 tgttgcctac tgttataaaa tatcctacac caacaataac cactataact actgctataa
     3001 tagtttcttt taatctactc ttcttagaaa caacctcttc atcatctaaa gaatcttctt
     3061 tatctgatac ataatttttc atttctttct tgttttctct atatttgtcc ttagaagaat
     3121 ttttagattt tttattacta ttttctttct ttttaatagg tattactact gatttttcat
     3181 tttctaattt ttcttctttt atctcttttg cttttttatc ttctaagttt ttactttctt
     3241 tttcaagctt cgattcatta tttgaagata ctttttcatc taagttatta tttattggtt
     3301 tttctatttt tacattacgt tcttcttcta tatgttgact tggttctttt tgttttattt
     3361 cttttaattc attttgctcc atttcttctg attttacttg atgattttca actggtttat
     3421 ctaaaattat atcaggagaa atatcattta aattctcttc ttcttgttca acttcttcat
     3481 tgtctttttt aataaatcca aataatttag ccttcttttt actcttttta ttttctggtt
     3541 caattatatc ttctttattg ctgtcgtact ctttatctgt agtatcttta tcttctacaa
     3601 cgtcacctat atcttgatta ttactatcta tttcttcttt aaccccttct tcagcatcat
     3661 cttctattga aatactactt ctaaagcaaa tatctttatc tgcatttttt atcatgtaaa
     3721 tgtattcttc aatatcgata tctttatttc ttctagttct ttttgatgca atttcatgat
     3781 tttctaattc actatagtta tcttttatta gattctgtgc tttattccat gtatcttcac
     3841 taatttctgt tttattttta attacatagt ccacaaattc cttaaaattt tcaggaccaa
     3901 attctccaat tatagtcagt ctcattgcac cttctataag gtctctattt cttctcttta
     3961 aaaattctaa tacaaccgtt gcacaagcaa aatctttaat aatttctttg ccaaaaccat
     4021 atccttcaac agaatgaagt tcttctaatc cacataactt cttatattca ttgtgtattg
     4081 tttgagatac actttcatta tttaagctat ttaacttatc tataaacttc gattctatat
     4141 catttgattt tgattcatgt tttacttttg aatttttaat ttcatttagt agcatgtcat
     4201 tttctgaatt atctttacct aattgcaaat cctcaatttc agttgctttt atttctactt
     4261 tgttttgttc gttattctct tccacattat taggtaccac atctttatta ctagctaata
     4321 ttttgtttac acagaatata atttctcttt ttaattctct tttcgcatct gcttgatttt
     4381 taagaaataa atctccacat tctttctttt ctaaacttat ttttctaaga gatatattaa
     4441 gaaggtcttt atataggttt aaaaccttat aatccttttc taaacgtctt cttctctctg
     4501 atagttccat tttatcataa gctaaaaaac tatttaaatc attggctttt agagagtcta
     4561 atccctcttt catagcctta tttacatact ttctacattt tgctgtagtt aactctaaat
     4621 ctttttctaa ttcatctatt tcaggtatgt acttatctac tatccatttg ccaataagtg
     4681 gttctgtatt tcttgctaat gtcttataaa acaatatatt aaattttaaa agctctgcat
     4741 attttgcttc attaaattct actttttcaa atttcttttc caaaacacca tctagctttt
     4801 catttatttt agactcttgt tttaaaaaac ttgctgtgac atttaaaaca gtgtttctta
     4861 ttatattatt ttcactcaaa accttcactc cttaaatcaa ttattttatt gtaatattta
     4921 cattgtgcaa agttgctaat ttgattatga tatctaaata attatttact aaatttgcta
     4981 catcttatat catatcatat tatttacatc attttacttt ttctttttat atctatctta
     5041 tattatacca tttttaatat ctgtactaac ttatattaca atattcctca aaattttaaa
     5101 agtagattag acaaatttac aaataaaaaa acatcagttt aaactgatgt ttttgactat
     5161 cgaaataatt aagcaagtct ataagcggag ttctgtatta gataatcatc tttctaggcc
     5221 tattgtcacc aataggctca agcgacctac ctaccggaga gtacgagcag cactctttat
     5281 tcctatcttg gtcttgctcc aggtggggtt tacatagcca actagtcgcc tagctgctgg
     5341 tgagctctta cctcgccttt ccatccttac cacaatagtg gcggtttatt tctgttgcac
     5401 tatccttagg atcgctccca ctaggcgtta cctagcaccc tgctctatgg agctccgact
     5461 ttcctcatgt aagattacat gcgattatct ggcttactta atatttattt tacttagtta
     5521 tgttaacaca aattctttta catgtcaaag taatattata ctaaaaattt aaatttattc
     5581 aaaaatagtg taatatagat atataaacta catttaactc gaggtgatct attgaaaaaa
     5641 ttatatagaa tagtaattaa cattatacta gtattagtaa tactttacag tggatttaat
     5701 atatactcaa aacttaccaa atacaatcat gatactaaaa tatcctcaga acttcaaaaa
     5761 aaagaatata aaaaagagga tttatcaaag attaacagtg attttaaatt ttggctttct
     5821 gttgagaata caaatataaa ttatccagtt gtacaatcta aagataattc ttactatctt
     5881 gacaaagatt tttataaaaa agattctatt tctggtacac tgtttatgga ttatagaaat
     5941 aaatctattg atgacaaaaa tatcataata tatggacata atatgaaaaa taaaactatg
     6001 ttcaacaacc taaataagtt taaagatgct gacttcttta aaaaaaataa taaaataaaa
     6061 ataaccttga atggaaaaga atttttatat gatgtttttt ctgcttatat tgtagaatct
     6121 gattatgatt atctaaaaac aaattttaac aatgaatctg attatcaaaa ttacataaat
     6181 gatattactt ctaaatcatt gtataaatct cctattaagg taaatagcaa tgataaaata
     6241 gttacacttt ctacatgtac ctatgaattt gatgatgcca gaatggtgat tcatggtaga
     6301 ttgatttaaa actcacagac acagtttaaa acaataattt taaactgtgt catcttttat
     6361 acctataatt tgtaaattag accactaaaa gtatttaata caaaataaca aatacatcaa
     6421 acaataaaaa acaataaaaa aagactatgc taaaaacata gtctttactg gagctggtga
     6481 taggaatcga acctacaacc tgctgattac aagtcagctg ctctaccgtt gagccacacc
     6541 agcacaccta ttttattgta aattagcaaa actaattttt aaaaatta
//
LOCUS       DAEMIK010000186         6552 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEMIK010000186 DAEMIK010000000
VERSION     DAEMIK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.189"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(111..443)
                     /locus_tag="KPK87_003434"
                     /pseudo
     CDS             complement(111..443)
                     /locus_tag="KPK87_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(544..1224)
                     /locus_tag="KPK87_003435"
     CDS             complement(544..1224)
                     /locus_tag="KPK87_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539544.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIIGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(1221..2147)
                     /locus_tag="KPK87_003436"
     CDS             complement(1221..2147)
                     /locus_tag="KPK87_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539545.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVEFAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(2220..3137)
                     /locus_tag="KPK87_003437"
     CDS             complement(2220..3137)
                     /locus_tag="KPK87_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2539546.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(3137..3826)
                     /locus_tag="KPK87_003438"
     CDS             complement(3137..3826)
                     /locus_tag="KPK87_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539547.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(4234..4529)
                     /locus_tag="KPK87_003439"
                     /pseudo
     CDS             complement(4234..4529)
                     /locus_tag="KPK87_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5345..5563)
                     /locus_tag="KPK87_003440"
     CDS             complement(5345..5563)
                     /locus_tag="KPK87_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539548.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTIVEELFEFIEENNI"
     gene            complement(5564..5971)
                     /locus_tag="KPK87_003441"
     CDS             complement(5564..5971)
                     /locus_tag="KPK87_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539549.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(6057..6545)
                     /locus_tag="KPK87_003442"
     CDS             complement(6057..6545)
                     /locus_tag="KPK87_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG2539550.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
BASE COUNT         2275 a         1053 c          637 g         2587 t
ORIGIN      
        1 tatatagtta aaatagtgag tatagtccaa gctaagaata actaagaaat tagcgttaaa
       61 taccatagtt aaaaatatat ttatatataa actttttttg ctacaaacaa ttatttccat
      121 aacattttat atacttctct aaaatcttct aaacttgata aatattcaat taaagggaca
      181 taattaccta aactacaaga atttaaggag tttctataca agtcctcact atcccaagat
      241 acaattttta taggaagatt attttctaac atctgtttta gcataacaaa tctacctatg
      301 tgaccattac catcttgaaa tggatgtatc aattcaaatc tataatgaaa ttcagctata
      361 actttaatag atacttcact ctgactatag taccattcaa ttaattcatc aagctttggc
      421 tcaacttcaa atggttgtgc aatattatta aaactctaat aatattaata atagctgata
      481 taatagcaaa tcattattct cccaagaaga tacaattaat aactaccttc ctatccacag
      541 tatctagtca atttcagtaa ctttatgatt tagtatataa tatataccaa tcacagttat
      601 accaactata ataaaaacag aaatcaaact gtcttgagtt gtatttgtac cagctaaaat
      661 aaattgtctt aaattgataa aaataattga actgacaagg gtaacaggta ttgattttct
      721 tatcattcca acaacgaatg tccatatacc taatgagcaa aatacaataa tacttttaat
      781 tgctcctggt atccaataag ataaaataga aatatgaaaa tctccaataa ctaaattaaa
      841 atacttatcc aacacaacaa cgaatgtaaa acaacagata tatccaatta tcattgatga
      901 tatcataaga gtcgtaatca aaagtaactt tgataaaata agttttttac ggtcaagtgg
      961 ataagagaat aaaatcaaga ttgttttatt tgtatattca tttattatat atgccacatt
     1021 caacacagca aataatataa tatagtaaaa acagaaaaat aaactgattg tcctaaatgt
     1081 aacatcataa tcatatattg aagtagattt atcattcaat ccagtaaaga caaaaaacat
     1141 acataagaaa atccccagca ttgaaaataa aaaatatctt tttagcccaa ctttttttag
     1201 ctctagtttc attaaatgta tcatagtgtt tctttacctc ctgtacctgt agtttggaag
     1261 aagtaatctt ctaaagaact ttgtttacga ccaatgctat ttataccaat ttgattttga
     1321 ataaatattt tggatatttc ttttacatct tttcgtaaat catatatttc aatacaactt
     1381 tccgaagtaa ttttaaagtc ttttattcca aattcttttt ctaacaagta tccagcaaat
     1441 tccacattat ctacatctac ttcaacataa tctgtttgat atttgtgtat atctgacata
     1501 gaaatttctt taataagttt accatcttga ataattccaa tagtgtccgc aatttgttct
     1561 acctctgaaa gaatatgact agaaataaat atagttattc cataatctgt atttaactta
     1621 cggaataatt ctcgcatttc tcttatcccc tctgggtcga gtgcatttat aggttcatca
     1681 agaatcaata attctggttt agtaagaatt gctctagcta ttgcaagacg ttgtttcatc
     1741 cctaaagaat attttgaaac tttcttgttt gattgctttg acaatccgac acattccaat
     1801 acctctgata tatgttcttt gttataataa cccatatatt cacagtgtaa atcaagattc
     1861 tgtttagcag tcaaattctc atataaatac ggattttcaa taatagtacc aatctttttt
     1921 aatatttcaa aatcagtttc tgccactttc ttactgaaca taactatttc tccagaattt
     1981 ggcttaacaa gatttagcaa cattttcatt actgtacttt tacctgctcc atttggtcca
     2041 acaaatccat aaatttctcc ctttttcaca tgaatattaa cagcattaac aacagcttta
     2101 tctttatata ttttctttaa ttgatgggtc tctatgatat agttcatcat actccctcct
     2161 ttctaaaaac agtatacaaa ataaaattat cttttctctc ttttaaatct aacttttttc
     2221 taacttttca attttaccgt aaaaactgtt tttacattag gtctactatt tacataaatt
     2281 gaacctccca tagagtttgc aagcccttga gaaatagcca aacctaaacc attgcctttt
     2341 tttctgtcag cagtataagc tcttgtgaaa atatgctctt tttcagtttc agaaatacct
     2401 gaacctttat cctcaatatc tataaaaaca tgtttttcct tttcatatag tgaaatccct
     2461 aaaaatctac catctgaacc atattttata gcattatcaa ttaaattttt gagtatacgt
     2521 tgtaatgctt ctgtgtcaac atttgcatat ataggtctat cttcaatatt aacttcaaca
     2581 ttaaagtttt ctttatctag caaatcataa aactctaaaa taatctcatg acataattgt
     2641 gttagattgg atttttgaag atttaatatc atatctccag attggatttt tgccatatta
     2701 aacaggttat ttattgaatt aacaagctca tttgaatttg tttgcatttt ttccacagtt
     2761 gctctcattg aagaagataa ttcttctttt tgactttgaa gatatagcat ttcaatataa
     2821 ccctttaata ctgtgatggg agttcgtaaa tcgtgtgata tgtttgtaaa tatttgcaat
     2881 atcgcttttt cacttctatt ataattaatt tgcctgctat aaaacttttc aacaagttta
     2941 tttatatcta ctgctgtttc ttttacaatc gaaatatcag atggtactaa aatataatta
     3001 atttcatcat catttgttat gtcacgaagt cttgaattga tatacttata atcactttcc
     3061 attttctttg atttatttcg ttcttttaag tagagagcaa atagtacaat cgaaattaca
     3121 actaaaaata actccattat tcttcctcca ttttgtagcc aattccccaa agagttttaa
     3181 tataattatt tttttctata ccatcagaca atttatttct taacctgtta atatgagtat
     3241 ttagcacatt ttcatttcca taataaggtt ctttccaaat agcgttgtat aattgttcct
     3301 ttgaaaaagc acgtccttga ttagtcgcta gtaatttaac aatttcaaat tctgtatgtg
     3361 ttaagtttaa gtcaattcca ttctttgaaa ctacatattt tgatatgttt ataatcaaat
     3421 ccttataccg aataaccata tcttgagagt ttcttgaact gctatacact gttgcacgtc
     3481 taatattagc tttaattctt gcaagcaatt ctacgattga aaaaggcttt gtaatataat
     3541 catctgcacc aagattaagc cctagcattt tatctgtgtc actatctttc gcagtagtaa
     3601 taataacagg aacaacactc acacttcgta tatattttaa tacttcaaaa ccactagact
     3661 ttggtaacat caagtccagt aaaataatat caaatgaata atttttaaat atttcaatag
     3721 ctgtatttcc atcaatggca gttttaacag agtaattctc actaatcaga aaatcgtata
     3781 ccatttctcg aatattctta tcatcttcaa ccaataagat gtccattatt attcaactcc
     3841 ttttcgtcag tatacttcaa atgatatatt tattaaaaag ggttgtcaat aaataactgc
     3901 ttatgatatt gtatctgcaa tttggtgata ctcttctaag ttaaagttct tttcaataag
     3961 ctttattgaa aaaagaatta aaatatagta tactctctta tctttatcta aaatatacat
     4021 aattttcttt tattaacatc tacaattaac tattttatat tataaaacac cttttaactt
     4081 ttgctaaaaa atattttata cataaaaaag aaaccacatt ctagttttat acagaacata
     4141 gtttctttaa aaataatcaa atttggattt taaataatta aaaacaaact tttatttgtt
     4201 tttaattttc caattttata gtagataatt tagttaatta gttcttaaat gtttatcttc
     4261 tttagtaaat ttttttctta ttaagaaata tgcaactatt acaacaagtt ctattgctaa
     4321 aaatacacca taagtagcta tagaatctat gtatctatgc catttctaaa gaaaattaaa
     4381 gcaattcctg ctgctataat acctacagca ttttaatttt gaccaaaacc ataaaaagaa
     4441 gggaaatttc cttttatttc aatgaatgtc aagattaatc caatagctat aaatagtact
     4501 cctacaactt ccataatcat gctaggcatt ttgacccctc cttattaata attaactact
     4561 actataaaaa tatttattat ttttataatt atattatata tgcgattaca tgtttttgga
     4621 agtatatttg gaagtaattt catatttatt taacttaatt tttttgtgat aaaataattc
     4681 taattttcat atagatatta ttataaattt tctagtttat ggagttattc attctataat
     4741 tcacaaatat gtatttttta atctattaaa ctattcattt tctggcttat aagcatggac
     4801 ttcttatcta taagcttgtt ttttcatcta ataagttatt tattctttaa tctatgagct
     4861 tgttttttca tctataagtc atttattttt taatctataa gtttagttct tcatctataa
     4921 atcatttatt ttttaatcta taagtttggt ttttcatcta taaataagtc atttattttt
     4981 taatctataa gtttagttct tcatctataa atcatttatt ttttaatcta tgagcttgat
     5041 ttttacctat aattaattca ttctctaatc tattagcttg gtttttattt ataagtcatt
     5101 tattctctaa tctattagct tggtttttac ctataattaa ttcattctct aatctattag
     5161 cttggttttt atttataagt catttattct ctaatctatt agctttcttt ttcatctata
     5221 aatcattcat tctttaatcc attagcttgg tttttcatct ataagtcatt tattctctaa
     5281 tctataagct tggtttttta cctataatta attcattctc taatgcataa gtaatacatg
     5341 aaatttaaat attattttct tcaataaatt caaacaactc ttctacaatt gtatcaaata
     5401 ccttagcaat atccattgcc agttttaaag aaggattgta gcgaccattt tctaaatgta
     5461 caatagtttc acgtcttact cctactgact ttgcaagttc accttgtgtc ataaggttct
     5521 tttctctata ggatttaatt tttgtcttta gtaatactga cacctaattg tatatcccct
     5581 ttctgtcaaa atatataaat agaattaact tcaacaagga ttgaataaac aaaatgacta
     5641 ataaagacat tcctatattt aaatttgaca catttatgtc caaagttaca agaaatatag
     5701 atactatagc tacagaaaaa aacaatactt ttattgcaat atcattaact cttgatagta
     5761 tacattttga tgcctcatca attaaatcat catacttttt attatagacc cactgagctc
     5821 ccatagctaa tacccaaata attataggaa gatattcaat actacttcct ttaaaaaaat
     5881 acttatttac taatgtagct ccaatacaat aagaaattcc caatatttct gaaataagtg
     5941 ttgatttaat accagtttgt tgtttagaca tataaaatcc cccttaaaaa gttatgtatt
     6001 tataacacct cttgttataa atatataact tttttgaata aatttcaata aaaatattat
     6061 tttgcaacaa attcttttat taattttaca ttagatagag gcactttaga tgtcactcct
     6121 tgcaatgtca aatcttctat attaacttca tttaattttg aacacagttc taagtcaaat
     6181 attctttccc aacttacctt cttttcaatt tgttcatctt cataaagctc tacagcaata
     6241 tccattaaag gtaaatgcca taaatcaaat tctgataata acacattatc ttcacttatt
     6301 tctatttcaa gtaaaacacc tttttttcct ttttctaaat aaccactaca tcttaaatca
     6361 ggtctcttcg tccaaaccca tatcatacta tcatcaaatt catatattct atttgtcatt
     6421 tgactataca tccatttgta tggaatatca aagtcttcta atataaaaga ataatcattt
     6481 gatagatatt tattgttttt attgttattc caaaattcta aagtctgtat agtataaaat
     6541 ttcatatata aa
//
LOCUS       DAEMIK010000187         6529 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEMIK010000187 DAEMIK010000000
VERSION     DAEMIK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.190"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            65..1807
                     /locus_tag="KPK87_003443"
     CDS             65..1807
                     /locus_tag="KPK87_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG2539551.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGNDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1971..2165)
                     /locus_tag="KPK87_003444"
     CDS             complement(1971..2165)
                     /locus_tag="KPK87_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539552.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2162..2743)
                     /locus_tag="KPK87_003445"
     CDS             complement(2162..2743)
                     /locus_tag="KPK87_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2539553.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2911..3729
                     /locus_tag="KPK87_003446"
     CDS             2911..3729
                     /locus_tag="KPK87_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2539554.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4471..5292
                     /locus_tag="KPK87_003447"
     CDS             4471..5292
                     /locus_tag="KPK87_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2539555.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5602..6381)
                     /locus_tag="KPK87_003448"
     CDS             complement(5602..6381)
                     /locus_tag="KPK87_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG2539556.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2449 a          890 c          837 g         2353 t
ORIGIN      
        1 atgcattttt tatatttaat atagtaaaat aattaataac tatatccatg attggaggat
       61 ttttatggtt aaatccaaga agaaaatatt tgtttttcta attttaattg cagtttttat
      121 tatatttgca tcatacataa gaaattatac taatgataca aaaaaagatt ctgttagagg
      181 aactattcct tttgaaaaaa tggaatataa gcgtcctaat atacagtcaa tttgcgaaaa
      241 cattaacaaa tgtaacgaca aacttttaac agctaaaaca gctaaggaac aattaaattt
      301 atttaatgaa gttgataaaa tttatcagga tttttacagt acacttacta tagcaaaaat
      361 tagaaataac atagattcgt cagacgaatt ttactctaag gaatataagt atcttatgag
      421 taaatcagta gatgttgaca tgtcatacaa agactttcaa gatagatttg taacttctaa
      481 attctctaaa gaattagaaa aagaacttgg aaatgataca tttaattatc taaagaacat
      541 tggaaaactt aattctaaag aagttgaagg tttgcttaaa aaagagcaag acctagttgt
      601 aaaatatgaa gacttattag ctaaatctac agtatcaata gatggtatag aggtagactt
      661 tgaagaagcc ttgtcaagac caaatcttag ccctgaagaa tatgtgaaaa tttattcaga
      721 ttacttaaaa aaatacaatc ctatatttgg taacatattc ttagaactta tacagacaag
      781 aactgaaata gcaagtaagc aagggtttaa gaattatata gattatgcct atatgaacct
      841 aaacaaagac tattctcaag aagaagctaa aaaatttaga caagatgtaa aagattacat
      901 agttccatta tatagagaga tttcatctaa accatctgat tcttctattt atattaaagt
      961 atataaaaat cgctctttta ggaagtttga taaagtactt gaagatatat ctccaaaact
     1021 aaaagagagc ttcgactata tgaaaaaata tgacttgtac gattattctt ctggaaaaaa
     1081 caaatcacct ggaggatata ctacttatat aaataaatat aaagcacctt tcttgtttaa
     1141 tacatgggac aattcttttt taggtgttac tagttttgct catgagtttg gtcattttta
     1201 taattactac aacagtatta acttaaataa taagatgcaa ccaagtatag atgtatgtga
     1261 agttcattca acatctttag aaattctatt ttataaatat tttgatgatt tttttggtaa
     1321 acaatctgaa gctataaaaa aagaacactt atcgattgtt ttaaatacta ttattgatgc
     1381 ttgtctttat gatgaatttc aagaaattat atacaagaat ccatatatga gtctaaatga
     1441 tatcaataaa ttgttttttg atttagagga agaatatgga gtttccaata atatacttag
     1501 agaaaaaaat gctccttttt ggatacttgt atctcataac ttccaagttc ctttttatta
     1561 cttgagttat ggtcttgcat ctgatgtatc acttcagata tggcaattat cacaagaaga
     1621 ttacagaaaa gctgttgatg tatatatgga ttttttaaat caaaatactg atgctggatt
     1681 taaagatgtg gtagaaaagg taaatttaca attacctttc caaaatggaa atttagagga
     1741 aataagctct actttatatg attattttgg tatagataat ccgcttgaac taaaaaatgc
     1801 ttcttaattt ctaatacttt atactcactc ttaagattta gtattagatt gaaataagtg
     1861 atattttttc acacacaatt aatgtttaat aaaattatat ggtaaagtat cagaaatttt
     1921 taagactctt gtctttataa tttctgatac tttatttatc aataatatta ttattttttt
     1981 atatatataa ttaaaaggat tgtggaaatc aataaaaata tccctgtaaa aatagcacta
     2041 ttcatatgga acttaataga acttgcatct gttaccttat tattcttaat ccagaaccca
     2101 caaaccatag ttgaaagaaa aagtaaaaca gtgataagac ttgaaccaat agttataatt
     2161 ttcatcttac atcaacatcc tctccaataa tattttctat taaaatatca atgaatttat
     2221 ctcgctcata tttattcttt atatttatac cactgtataa ctgaaatgat tctgtagata
     2281 aagcagaaat ctgtattggt aaaataattt gcagggcaat gattcttaga ctcatttcat
     2341 cttttttatt tccaaaaatt ttacgaagca tagggacaat atcaagttct gcttgcatat
     2401 ctccattggt tatacattga ttcaacacaa aaggaagcac tttttcataa tgtaatccag
     2461 tatcacacaa tttttttaat aaatttttca aatcttcaat aggtcttaga gtatatacac
     2521 tatcgtcata tagctctgct attatatttg acatcacatc tcctatggca gtactcaaca
     2581 aattatcctt tgttttaaaa tgataattta ttaaaccaac tccaacattt gcacgctcag
     2641 caatttttct aactgtaatc tcctcaatat catctacttc atttaaaaga ttcaatgtaa
     2701 cttcaattat acgttctcta gcatctttat cattactttt cataatagac ctccaaaaca
     2761 attaacattt ttggttctga acatgttcag tataatattt ttttcatcat atgtcaatat
     2821 aatttaatta taattttagt tgactctaac attatgatat ggtttatatt aatattaagc
     2881 taaaagtacc tttataaagg agacaaaaac atgtttaaga taggcgattt ttcaaagcta
     2941 agtaaaatta gtattcgtat gttacgtcat tatgatgaaa taggactttt aactccttct
     3001 catacgaata aaactaatgg atatagatac tatagtgcag accaactttc tactacaaac
     3061 agaatccatg cactaaaaga tatgggcttt ggcttgtatt ctataaaaga aattttgaca
     3121 gaatataacg ataaagaaag tctcataaaa tatctaaata tccaccattc tcaagtaaaa
     3181 gaacaattag aagatacaca gaaaaaacta ttaaaaatag aaaccactat taaaagaatt
     3241 ggaggaaatg atattatgaa aaattatgat gtaactataa agaattttgc accaaaatat
     3301 atgatgacac ttagaagggt tataccaact tatcaagacg aaggaatgtt atggcatcaa
     3361 gcatttttag agacaaaaga ccagaatgtt caaattgaac ccccaaagta ttctaaagca
     3421 gttttttatg atactggata taaagaagat tatgtagatg ttgaagtaca agttgctgta
     3481 tctggaaagt ataaagatac tgaacatgtt aaatttaaga ctgttccaag tgtgactgct
     3541 gcaactgcaa ttgttaatgg aaatttcaat caagttgcag acgcttgtga ggctattggt
     3601 aattggatat cagataataa ctatgatgtg gatggtccta tgtttaatat ttatcatgta
     3661 agtcctggca acgatagtaa tcctgataat tgggtaacag aggtatgctt cccagttaaa
     3721 aagaagtaat ttttatatta attggataat attttaattt gttagacaaa ttacttttag
     3781 tgttatgtat aaatacctac aatttgatta ggatattatc ttaatttgtt aaacaaatta
     3841 cttttagtgt tatgtataaa tacctacaat ttaattagga tattatctca atttgtcaga
     3901 taaatcaatt atgattttat gtttaaatga ctagaatttg attaggatat tatcttaatt
     3961 tgtcagataa atcaattata agattatata ctatccttat actctagtta aatatatttt
     4021 aatcactaat ataaattagt ttttttaaca aaacaataaa agagtaaagt acaaataatt
     4081 cctttgtgct ctactctttt taactttctt tagacaataa tacaatgata aatgtaaaaa
     4141 attatatatt ccattctata gaaatatgct caccaataac atgtatgttg ttaatcaata
     4201 tattatatca aattatatca aaattacata aaccttatag gacaatcata tcttattaca
     4261 agaaaaatgt cttatccata ttgaataagt cattcagttc atatacaaag tcaatttcat
     4321 tgagaaacat ttaatatatg tttataaaaa tttcatacta ttctttcata tttgcatgtg
     4381 atataattta ttaactcata attttgttaa tcagttgtgc ttgtatatta tttatataat
     4441 ctaatatttt aagaaggtgg tgtaataact ttgaaattaa aaattgttgg tagtggcggt
     4501 atgtcaatta ttccaagttc attttgcaaa tgcaaaatat gtgaagaagc cagacaaaaa
     4561 ggcggaagat atgaacgatt agggccaagt ttatatattg atgatataaa aatgctgata
     4621 gacacacctg aagacattgc agtagcttgt aacagacaaa aaatatcgga agtaaaacat
     4681 atttcaattt ctcaccatga cccagaccat gttaagggta ttagaattgt cgaaaaaata
     4741 ggatgtgact ttatcacagg agaaaataat ccaattggct tttatgcgct tcctgaagta
     4801 attgaagata taaaccgaac gaatctcgat tgtcttaaat attatggtga tgttataaat
     4861 tgcatttcta ttaatgaaac ttcgcatgta aagataaata attttgatat tgatttgatt
     4921 aacaataacc cagacaggaa tataaccttc tatgttatca aagagaatga aaaaaaagtc
     4981 atatatgcct gctgtaatcc caagccattt actcatagtg atatgtactt tgatgctgat
     5041 attttaataa taagtctagt ttctgatgat ggtatattaa atgatggtac aaagcttgaa
     5101 gacacaccat ttaaagatga aatatttact ttagatgaga ttgttgaaat taaaaactat
     5161 tatagaatta aaaagattat cattacacat attgacgaaa tgtggggaaa atcatatggt
     5221 tattacagtg aattagaaaa gaaacttgat aacatttatt ttgcatatga tggtatggaa
     5281 attatagtct aaatcattaa gtaataagca taatgcaaaa taagaaaact tcaatttaac
     5341 agtatagtta aactatgtaa tatcaacata tctcagtttt tacaaaatat catagtattt
     5401 atctttataa aatttcaagt cctacatatt ttaatacttc aatatctaaa taaagtaaaa
     5461 tcaaaaatat attttatata cattatagac ggcttatcgg cactagataa gccgttttgt
     5521 cacaattatt atttgctcaa caaaacttct cataatatat aacagattga atctccaact
     5581 gatacttaat taccgaaact cttattcctt aatatgttgt tgcattaacc aaaccttcat
     5641 catcattttt tgtggcaata gttttgctaa taaatgactg gattttgtat ttataccata
     5701 cacagaaata tccttatttt tctttgcatc cttcaatgct tttttggcaa caatgtctgg
     5761 agacactata cctgggaaat tttttgtccc tttttcagct acaactatac ctctcttaaa
     5821 tagatttgta ctcatccaac ctggacatac agcaattgca tttatccctt tactcttaag
     5881 ttcaacattt aatgctcttg tataatttct tataaacgct ttagttgaac tataaatatt
     5941 ttgatatggc aatggctgga atgatgcttg tgaagaaata ttcatgatat ggcttccctt
     6001 ttgcatatat ggaatacata taagtcccaa tgctacaaca gcactaatat ttaaatcaat
     6061 catattaata gattcatcta tacttaaatc attgtatgaa cagaattttg caaaaccagc
     6121 attgtttata aggtatttta tacatacatt ttctttactt aaaaatgtac caatttcttt
     6181 tacttcttct atttttgata aatcttttga aaaaattttt accttacttc caaattcatc
     6241 tactaattga tttaatctcg ccttgtttct tgcaactgcc catatttctg ttatttcttc
     6301 ttcatttatt aataacttta gaaactcttt accaaaacca ctattagcac ccgttataat
     6361 tgcaatcttc ttataactca taattattct cctttaccat aagtttatac gctcaaactg
     6421 taaacttatt ttttacaaat aaaacaccct ctattcagat gttatattca tattatttga
     6481 gatattatat ttatatcatt ttcaataatt gttatatagc ataaaaaat
//
LOCUS       DAEMIK010000188         6505 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEMIK010000188 DAEMIK010000000
VERSION     DAEMIK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.191"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            276..925
                     /locus_tag="KPK87_003449"
                     /pseudo
     CDS             276..925
                     /locus_tag="KPK87_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
     gene            1150..1821
                     /locus_tag="KPK87_003450"
     CDS             1150..1821
                     /locus_tag="KPK87_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539557.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1818..2948
                     /locus_tag="KPK87_003451"
     CDS             1818..2948
                     /locus_tag="KPK87_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2539558.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3380..4225
                     /locus_tag="KPK87_003452"
     CDS             3380..4225
                     /locus_tag="KPK87_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539559.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4225..5253
                     /locus_tag="KPK87_003453"
     CDS             4225..5253
                     /locus_tag="KPK87_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539560.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5256..6269
                     /locus_tag="KPK87_003454"
     CDS             5256..6269
                     /locus_tag="KPK87_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539561.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKYSILKGFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT         2431 a          642 c         1122 g         2310 t
ORIGIN      
        1 atggatagta tgttatatgt actaatttat atgacattat tggtatctat cattggaagt
       61 atattttatt tttgtaaaaa cctcttaaat cataaaatta agtatgtttt aataagagac
      121 atatttgtaa atagaattac atattctgaa gaacggttgc ctaaacagta tatagttttt
      181 cagaaatatg atatttggag gtattgtagt ttgttttaat ttgaaatgaa tctattataa
      241 attctaaagt ttaaaaataa aaaggagaga ataaaatgga tttcatttca agtatattgc
      301 aaggattatt aagtatatta tttaatttta caggtgattt tggaatttct attgtactta
      361 taactttaat cgttaaatta gtacttatgc cactatcatt aaaacaaaga ttttctgtaa
      421 aaaacagcaa gaattagctg aaaaaatgga acatataaaa gaaaagtata agaatgatac
      481 taaagaattg gaaaagcaaa tacaagtaag ttctgtagaa agtatgaaga gtatgctggg
      541 atgtcttata gttttggttc aaatgccagt agtatatgct ttatatcatg tatgtttaga
      601 tatgacaaag gaatttacaa caattataat tccatggatt gctaatttag gtacatttga
      661 taatttgttt atattacctt gtatatatac attaattatg ttagcttcaa atttagtgaa
      721 ttatatacca tatttaaaaa taaattctca agtaaagttt aacaaacaaa tggctgtggt
      781 gactaccata acaagtctta tactaactgt tagaactcca gtagccatag gtctgtactt
      841 tataacaagt gcaatttatt cattaattga agatatttgt tttagaatat attttaatcg
      901 aaaaaataga atagtgacaa aataatataa ttattattag aaatatttta gcctgctaac
      961 atagaaaaag ttagtaggct atttttatct atgatatcaa ttccattaag aatagtctaa
     1021 aaaatactat caaaatgcta taaaaaatat tataaaaata ctatcaaaat actataaaaa
     1081 aatagttgaa aaataaataa attaatataa actaaaataa agcctacaaa aaagaggtga
     1141 taaatagaaa tggctaaaat actaattgta gaagatgaaa aaaggatgca aagtattatt
     1201 gtagaatata tgcaaaaggg aggatatact tgtataactg ctgatgatgg agtcgaggca
     1261 cttacaatat taaagagcaa caatatagat ttaatgatat tggatattat gatgccgtat
     1321 ttagatggat tttcagtgtg tagagtttca cgtgaaatga ctaatatacc tattattata
     1381 ttgacagcta aaggtgaaga ggaggacaaa ttaaagggtt atgaatatgg cgcagatgat
     1441 tatataacaa aaccatttag ccccaaagta cttttagcaa aagttaatgc cttgttacgt
     1501 agatacacta ctgatattcc caaaaattca ttgagtttag gaaagatttt tattatggta
     1561 gcatcaaggc aagtgtatgt agaagataaa ttgattgatt taacatataa agaatttgaa
     1621 ttattgcgtt tatttatgga aaaccctaat caagtttttt ccagagagaa acttttaaat
     1681 tgtatatggg gatatgattt tgaaggaaac acacgaactg tagatacaca tataaaaacc
     1741 ttacgcaaaa agcttggtag tgaaggacac catatagtta cattaattcg ttcaggatat
     1801 aagtttgagg ttaaggaatg aatagtttaa gaaagattat agaaaaactg aaaaatgtag
     1861 atatttcaaa ttggattaaa caaaattttg gactatattc agttagaaaa aaagtgtttt
     1921 tactttctaa attatcagga gcgttaatta tattatttta tctggttgtt gaagaatttt
     1981 cgataaactc taaatttgaa ttttggattt ggtttataat actggttata tttattatga
     2041 gtattgattt cttattggga aaatttattt cagaaccaat cacatctata aataaatctg
     2101 caaaaagtat gtcccaactt gatttttcta atccatgtac tgtaaatact aatgatgaat
     2161 ttggagaact ttctaggagc ctgaatacaa tgtctacaaa tctacaacag gcattatcag
     2221 acctagaaag tgcaaatatt caacttgaaa aggatgtaaa taaggaaaga atgctattgg
     2281 aacaaagaaa ggagttggta gatacaattt cacatgaaat gaagactcca cttggcatta
     2341 tacgtgcgta tacagagggg cttatggatg aagttgatga agaaaaaaag aaaaattata
     2401 tgaatgttat aatagaagaa actgatagaa tgaacaatat gattgtatca ttattagact
     2461 tatctgcatt ggaagcagga gtttctaaac ttaacccaga gcgttttgat tttatagagt
     2521 ttgttgaaac tgtggcaggt cgtcttttaa ttgatatacc agatgccaat ttttatttta
     2581 catatgattt gccagagtat gaagtttttg tagtggcaga taaaatgaga atggaacaag
     2641 tagtagagaa tctagttatt aatgcaaaga agcatgttgt atataatggt aatttagatt
     2701 tatcgattac atgtgaaaat ggcttgcttt tatttgaaat ttataatgat ggtaggttta
     2761 tagagcaaga tgagatttca aagatttggt ctaagtttta tcgtagtgtt caatctcagc
     2821 gtacaggagg ttcaggtcta gggcttgcaa ttgtttcaca aatacttaca atgcaagggt
     2881 tagaatatgg agtgaataat cgtgatagag gagtagagtt ttattttatg attccaattg
     2941 atgaataatt tagtattaat ttataaatca taagtatctg atgtaaaata tttttatttg
     3001 tatatgtaag catttaaaga gaataaaata aaaaaactta atgaaaaaag cttaatgaaa
     3061 aagtctaata aaaagtttaa taaaaagctt aataaaaagt cttgttttcc tgatgaaaac
     3121 aagatttttt attaagcttt ttattatttc acttcgattt cacaagagag acactctgat
     3181 ttcaccaaat agaaataaaa taggttaaat aaattaaaaa atagaagcgt aattaaataa
     3241 ggttacaagg gggagtactt atgaaatggt atacatggtt gactattata ttgataatat
     3301 tgctttctat tctgcttaaa attaaattta taaaatggtg ggatagacga caaaaaaatg
     3361 ataaaggagg ttatgataga tgataaaagt aagagagtta aaattttctt atggaaaaga
     3421 taaacagata ttgcatggac ttaattttga agtaaaagaa ggagaaatct ttggattcct
     3481 tggacctaat ggttcaggaa aatccacaac acagaaaatc ttaaatggcg ttttaaaagg
     3541 ctacaatggg cacgtatcca tttttgggaa ggaagttaaa gcatatacag agtctctata
     3601 tcaaaagatt ggtgttttat ttgaatttcc ttatttatat accaatttaa gtgcaattga
     3661 taatttagaa tatttttcgt ctttttatcc taaaaatcaa aggagagaca tatatgaact
     3721 cttagatttg ttagagttta agaaagagtt tataaataag cctgtatcat cctactcaaa
     3781 aggtatgaag caacgtatta gcatggcaag agcattggta agtaatccaa agttgttatt
     3841 tttagatgag cctactagtg gacttgaccc atctggagct gtactatttc gtaagattat
     3901 tgaagaagaa cgaaaaaaag gaacaactat ctttttgact actcataata tgctagatgc
     3961 agatttaatg tgtaacaatg tggcttttat tgcagatgga aagattatgg taatagataa
     4021 acccaaaaat ttaaaaatga aaaatagcaa taataaagtt gaagtggaat ttgtatataa
     4081 tggaaatcga gatattgaaa ttgtagatat acaagagtta gaatctggaa taacttttaa
     4141 atatgatgaa atcttaagtg tacattcaaa agaacctaca ctagaagaag tttttattaa
     4201 atacacagga aggatgttag tgtaatgtat tctaaatttt ttaccttagt aaaaaaagat
     4261 ttttggatga tgatttatgg taaatttttt gtgctgacac ttggctctct catactttat
     4321 tcgtgttata ttaattttgg ttatgtaaag tttatggatg aagatagtta tcacaataat
     4381 gtatatctat ataatccaga gaatataagc atgagtgcat ctccattaat taatcaagtt
     4441 tcttctatag gtgaattaaa agcaaaactt gatgaagata caagtgcagt ttgtattgat
     4501 atgagttcca gtaagccgaa agtaatttta catgaaagta ttaaaggagt tgacaattat
     4561 agagctgact atgcattgtc attattatca tcagaaaaag accataatag caaaatatca
     4621 gacaacaatg caataattca aattaatgat aaggatacat ctgagataaa acaaagaaaa
     4681 gacatgactt gtgagatttt attctttgaa attgtagcta ttggattttt agggattgcc
     4741 tctttattat ttaaagaaaa gcagatggga gtaatgcgta ttactggtat tttacctgta
     4801 aaaagaagtc tttttatatt atcaaagata tttgcttttt ttatatgtga cctatgtttt
     4861 acagttcttt tagtagtatt taatattgga tttttagaag gaataaaaat acttccacaa
     4921 gtattagttc aagctagtat tttatcaatt attatgtctt tgattggaat aggatgctca
     4981 atgttattga gagattttaa gcaattttct cttatatatc ttgtggttgc tgtattcata
     5041 actacaccta tatttctatc tgcaaataca tcttttgaaa tagattggat taaatatcat
     5101 cccttctatc atgtttatag tcagcttaaa aatgcgtttt tcatgaattt ttcaaaagaa
     5161 ttagtttatt ttgtagtgtg ttctttgaca ataatagttc tatttttact tgtaaaatat
     5221 atttttaata aagagttggt tagggaggga taatcttgat aggattacgt taccagttaa
     5281 agagtataaa aaaagataaa atgtgtataa tttccttctt tttaccagtt gttatggcga
     5341 ttttaataaa ttttatggga acaattgata tatcttcatt aggagagata cgttttggta
     5401 tagtgaagga tacacttcct gtacaaacag agcagtggtt gaagaagtat ggtatggtaa
     5461 gtgtatatga cacaaaaatg catttaatac gtgaaattaa ggatattaaa acggacacaa
     5521 ttggagttgt tagagatggt aaagggataa aaactgtgct ttatggaaat gagattaatg
     5581 ctataaaaaa tgttggaaag acattgccag agatgtttag actaaagggt gatttaaaag
     5641 atgttgaggt tacaatattg cctaaatata gtattttaaa agggtttcaa aatgttttta
     5701 ttgctatgat tttaattact gctatgttta tgggatgcac ctttaatgca atgaatatta
     5761 tatcggaaaa agaagatgga atatcattta taaatgaagt tttgccacag agtagaagtc
     5821 agtatattat acaaaaagta tttttgggtc ttttatgtgg gtgtttatct tcaatgataa
     5881 ctgtatttat atgtttggat atttcaatat tagatatgat aatgatgttg ttactcgttg
     5941 tattttcagc atttatttca tcacttatag gtttatttat tggaaagttg tcagaaggtc
     6001 ttatggtagg aattgtttat attaaaatag taatgatttt atttataggt actcctttgt
     6061 taatatatct tctaggggtt aataccagtg gtatagggaa tttatgctat ataatgcctt
     6121 cagttgctac atttaatggt attatggaga ttattaatgg taatgtcatt attatcaaag
     6181 atattgtaat tttggctgta cattgtgtag tatggtttat gatatatatt attttagata
     6241 aaaaaagaaa gttatctttt agtagataac tcagtataat tataattggg acaatttaat
     6301 aggtgattta atatactttg tatagtgtaa tttatgtagt gcaatttgta tactttaatt
     6361 tatgtagtgt aatagttagt tttattttat aattattcaa tataatttag taagaagttc
     6421 agtacaatta gagctactta gtggaaaact taataggtac ttaaactata taaatacttt
     6481 attttcctcc atttgtacta taaat
//
LOCUS       DAEMIK010000189         6299 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEMIK010000189 DAEMIK010000000
VERSION     DAEMIK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.192"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            246..1160
                     /locus_tag="KPK87_003455"
     CDS             246..1160
                     /locus_tag="KPK87_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG2539562.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            1550..2197
                     /locus_tag="KPK87_003456"
     CDS             1550..2197
                     /locus_tag="KPK87_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG2539563.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMSVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(2364..2933)
                     /locus_tag="KPK87_003457"
     CDS             complement(2364..2933)
                     /locus_tag="KPK87_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2539564.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(2938..3837)
                     /locus_tag="KPK87_003458"
     CDS             complement(2938..3837)
                     /locus_tag="KPK87_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2539565.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLNILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(3848..3997)
                     /locus_tag="KPK87_003459"
     CDS             complement(3848..3997)
                     /locus_tag="KPK87_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539566.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(3990..4574)
                     /locus_tag="KPK87_003460"
     CDS             complement(3990..4574)
                     /locus_tag="KPK87_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG2539567.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            4963..6288
                     /gene="cdeA"
                     /locus_tag="KPK87_003461"
     CDS             4963..6288
                     /gene="cdeA"
                     /locus_tag="KPK87_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG2539568.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
BASE COUNT         2261 a          920 c          774 g         2344 t
ORIGIN      
        1 acataattct cataaaaatt tctagtatta cttgtactct taataaataa tagccaacaa
       61 gtaccttaaa tcattatatc tatatctaat aagaaacaca gtaagataat tttttcgccc
      121 ataacttaat aaaaactgtt gatattagta gcaattgaat ttagttaatt tatttataat
      181 atacataaat ttaaatatta cgaataaagt attataaata ggatactttc taaagggagg
      241 aaattatgat agataatcaa aaatatgtta ttttatcact agaattacat ttattttttt
      301 caagaattat gaaagagcat gctctttttt tagaagcagg attcacaaat aaaaattata
      361 atcttgctat ggaagctgac cactataaaa agcaatttga agatttatta tcatacactg
      421 ttagtgctag taatggtata attagacctg atatattata ttcagaagag cttgtaacta
      481 ctctcacatc agttgcagaa caaaaaacag aagagtttac agggatagaa ataaacaaaa
      541 acatcactac aagagaatta aatctacaaa gtggtgtaaa cccacaagtt ggtcaagact
      601 tagtgaacta tgtagctcaa cttaactctg atgcaataag attacttgat gggcttatta
      661 attttaaaga aagagtctta gatggtgtac tatcatgtac tatatttaca tcaaactacc
      721 ctctacttct tgaacatata atacatgaag caaatttata tcgttcttat gtagttgacc
      781 ttgaaaataa aatagatatt gagtcaaaaa acgctaaaga aatagaatta ttctgggacc
      841 atattatgat ggaacatgct ctgtttatga gaggattact agacccctca gaaggtgaac
      901 taataaatac ttcaaatgat tttgctataa aatttaatga attaattgaa aaaacaaacg
      961 aaatgactga ttctaatatc aagaacatta cagaagaaac tctaaatgaa actgttgagt
     1021 ttaaagattt taaagaagca ggagcatcag gaatagaaca gtgtaagata aaatctataa
     1081 tattaccact tttagcagac catgttttaa gagaggcaaa tcattatatt agaatattgg
     1141 agagttataa aaacatgtaa ttaaaaatta cttttaaaat aatctttgca tatttagata
     1201 aaataaaggt acagttaact gtacctttat tttttataaa cttataaaat ttaaacaagt
     1261 tattcataaa ataaaataat aatcatctat aacccacaac tttattttat attaaataaa
     1321 aagtattagt gattatgttt tataagtatt tctttattca gttatttatt aaaaactaat
     1381 agtaaatctt atactaatcg catatttatg ttataactat attgtaacta ttttcgtaat
     1441 cttaggaaaa gattacttat aaaattaacc ttatacaata atatatcgta taatcttaat
     1501 tagcataata gttacaatat taaaatttaa atatgggagt gattttagta tgataagaca
     1561 aacaccatta gagaaacgat atgacaagct aggtcctcat attgtaacag cactcaaaaa
     1621 acgttatttt gatgcttatt actgtaaaac tagaaatgaa gctttgcaac aaattttaga
     1681 tttaattcct caaaatcatg tagtatcatg gggaggatct gaaacactta aagaaatgag
     1741 cgtacaaact gctgttagag agaaaggatt taaagttatt gatagagatt tagcaaaaac
     1801 tccagaagaa agaactgaaa ttatgcgtca agcacttcta tgcgatactt ttttgatgag
     1861 tagcaatgca attagtgaag atggacaact ctttaatatt gatggaaatg gcaatcgtgt
     1921 agctgcaatg atatttggtc caaaaaatgt tatagttgta gctggtatga ataaaattgt
     1981 aaaaaacatt gatgatgcaa tacaaagaac tagaacaatt gcatctccag cagttgttca
     2041 gcgttttcaa gatgtcaata caccatgcta tattaatgga agttgtatag attgcacaag
     2101 tcctgaaagc atttgtgcat acatggttac tacaagaatc agtcgtccag caaaaaaaat
     2161 aaaggtaata ttggtaggag aaaatcttgg tctataaaat atacatacat gtaaatagct
     2221 ctataaataa taaaatcata tgaaataact tatttctaaa agtggtagaa taaaagcttc
     2281 tacaagactt tcattctacc acttttatat tcttttaatt tgtgtttcta tattattaat
     2341 tgctccaatt ttcatatata ttactaacta tttttatttt cataattttt ttgttcttca
     2401 tcaagctgtt tataataatc tttattatag tcagctagat aatctatacc acctcctgta
     2461 atcacattta ataatttcat tgcagattct tgattaactt tacaaacata attatcacca
     2521 gatttaacaa gctctaatgt aatattaaat tcttttttct ttgcagaatc aatatctttc
     2581 atagttggat ttggcatcat ttctcctagt ttaggaggat tcttttttaa aatagaaacc
     2641 atatcagtat tgttaatttt taaatctaca gtcactttat ccttattatt ctttatatct
     2701 gaaatttccc aagtcatatt tttagaatac attttaaatc cttctttatc ttcgtatata
     2761 agattaccta accctgattt tgggaaatac ttatcaacag tatctgcatc ttgtgttttt
     2821 attgaattaa acatactatc cacttcattt tttatcttat tatcattagt acaaccagct
     2881 aacaccagta ctgatattgc aaacattata atacatataa taaatttctt cattaaatta
     2941 tatcccccta tacttttttg cacaaatatt attctctttt ttatcttttg aaaaagttgt
     3001 agtaattgca ttttgtggac aaacttttat acattcacca cacctaatac agtctggact
     3061 atttacatct ttatatattg gtatatcaag cttacatttt gtttgacatg ctgtacaatt
     3121 tgtacattta tctttatcaa ttttaaatct atatattgaa ataggattaa aaaaaccata
     3181 gattgctcct aaagggcata tatacttgca aaatggccta taagtaatga ttgacatcaa
     3241 taatatcaat agcaaaatat ttaacttcca gctaaataaa aatcctattg attctcttaa
     3301 attctcattt cctaaaataa gtggtattcc tcccaaaagt gttcctgaag ggcaaatcca
     3361 tttacaaaac caaggatttc caccccctgc tacatttaca atagcaactg gcaaaataat
     3421 tacaaacatt attaagacta tatactttag atattttaga tatttatcac cataaacttt
     3481 ttttatcttt actggaaagg gtactttatg tagtaaatct tgtacaagtc caaagggaca
     3541 aagccaacca caaacaaatc tgccaaaaaa agcaccaaat gccattaaaa aaccaacaac
     3601 ataaaatgag aatttatact ctctgcttga taaaactgct tgtaatgaac ctattggaca
     3661 tgctccaagt gcacctggac atgaataaca attcaatcca ggtacacata attttttact
     3721 ttctcctcca tatatagttc cttctaaaaa tccatttaca tatccatttg ttaatgctgt
     3781 aaatgcaact tgagtaataa gtcttatatt attatttacc ttagataata acttcattcc
     3841 tcttctccta accaatacca atacactcaa gacaaatcat aattgcttta gttaatactg
     3901 tatttgtttc tccacgatat gtacctaatg caataaatat aatactaatt gcaataatag
     3961 aaatgccttt tttattatct ttaataaaat tactcattgg cttttaccat ttttagtctt
     4021 tcatctataa tttttttcca atcttcctta ctatttgcac ctacaacctt ctcacccaca
     4081 aagtttcctt tcttgtctac aaaataagtt gttggaaact ccataactct tttaaaatca
     4141 gtttttttaa gttcttcaga tacagtcaat tgttggtaag ttgcatctga ctttttcata
     4201 atgtctttaa ctaaattttt ctcttcattt gaaagaccag ttctcatatc agttttatca
     4261 acttctacaa ctaatccctt tatagctaca ttggaattat tactctcata ttcttttgat
     4321 aatgcttcaa gctctggcat ttcacccaca catggtccac accaagtagt ccagacatta
     4381 ataagagtaa gttctttatt tgaaaatatc tcttttgtta ccttatttcc atcaacatcc
     4441 tccgtatcta aattactaaa ccattcacta ttattcttgt ttttttctac tggcttagat
     4501 tcttttttgc tacatccaga aatcatacct aaaacacaaa tacatcctaa aactactatg
     4561 aaaaaccttt tcatatctca cactcctaga atttatatag tatataaaat cgctgcttga
     4621 aataaaaata acttatcttc tatattaaag taattatatt taataaataa gagctaattt
     4681 aaaaaaagat atacagttaa aatctatttc ttttcagaca aaatcttata tatatataat
     4741 agcttgtaaa tcttaagatt atatacatat atttctaaag attttcttaa gaaacagtta
     4801 gattttacta atataagttt attaattagc attatactaa tttatcttaa tatgattttt
     4861 tgttgatttt aagctgttta tcgtataaat aaaatttttc ttgactatat agttactata
     4921 tagcttatta tatatagaga gtaataaaag gaggtaaaat ttatggaaaa tttatttaca
     4981 agaaaattca ctacttttga atttctaaaa tttgtttctc ctgcaattat atccatgata
     5041 tttatatctt tgtacacaat aatagatggc atctttgtat cgacattagt tggttctgat
     5101 gctcttgcta gtataaatat tgtactacct ataattaacc ttgtttgtgg atttggaata
     5161 atgatggcaa ctggtggagg tgctatcgtt tctatacgta tgggtgaaaa tagacaggat
     5221 gaagccaact ctacattttc ttttatagtt ttgttttcat tgattgttgg gattttattc
     5281 acagtaatct catatttctt tatcaaagaa atatctattt tgcttggtgc aacagataag
     5341 ttattaccat attgtataac ttatggtaaa gttatgattt tatgtacacc attttatatt
     5401 ttaaaattta tatttgagta ctttgcaaga actgatggaa attctaaatt tagtttattt
     5461 ctatcagtca ttggtggtgt aacaaatata atattggatt atgtatttat taaatatttt
     5521 ggaatgggtc ttttaggagc tgcagttgca actgctatag gtattatttt aacttgtgtt
     5581 ttaggtatta tttacttctt atctaataaa tctacactaa aattaagaaa accaaaaacc
     5641 gattttagac ttataagaga tactatgata aatggttctt ctgaaatggt tacagaatta
     5701 tctacaggaa ttacaacatt cttatttaat gtagtagctt taaaattagc aggagaaaat
     5761 ggacttgctg ctcttactat agtattgtat gctcattttt taatgacatc agtctatcta
     5821 ggattcgctg ctggagtgtc tccattaata agctataatt ttggtgctga aaacagtgat
     5881 aaattaaaag aaacatttaa acattctcta aaatttatat ttgtttcttc tcttttagtg
     5941 tttatttttg ctttagtatt tgcaccattt attgttaggg tctttgtaag tccagataac
     6001 acagtattta aactagcctt acaaggatta aaaatatttg catttgcttt tttgtttgtt
     6061 ggtataaata tatttgcatc aggatttttt acagcatttc acaatggaaa aatttcagct
     6121 attatatctt ttagtcgtgc ctttgttttt ataatcatag gaatcataat tcttcctcct
     6181 atgttaaaca tgactggatt atggcttaca gttccatttg ctgaggttat aaccatattt
     6241 atatctattc tatttataaa aaaatataaa tgtagatata agtattaaaa attaatact
//
LOCUS       DAEMIK010000190         6289 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEMIK010000190 DAEMIK010000000
VERSION     DAEMIK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.193"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            109..612
                     /locus_tag="KPK87_003462"
     CDS             109..612
                     /locus_tag="KPK87_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG2539569.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            659..1618
                     /gene="moaA"
                     /locus_tag="KPK87_003463"
     CDS             659..1618
                     /gene="moaA"
                     /locus_tag="KPK87_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG2539570.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1622..2092
                     /gene="moaC"
                     /locus_tag="KPK87_003464"
     CDS             1622..2092
                     /gene="moaC"
                     /locus_tag="KPK87_003464"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG2539571.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2128..2559
                     /locus_tag="KPK87_003465"
     CDS             2128..2559
                     /locus_tag="KPK87_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG2539572.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
     misc_binding    2990..3201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3364..4653
                     /locus_tag="KPK87_003466"
     CDS             3364..4653
                     /locus_tag="KPK87_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG2539573.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            4707..5786
                     /locus_tag="KPK87_003467"
     CDS             4707..5786
                     /locus_tag="KPK87_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG2539574.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYGASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            5807..6172
                     /locus_tag="KPK87_003468"
                     /pseudo
     CDS             5807..6172
                     /locus_tag="KPK87_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT         2437 a          626 c         1217 g         2009 t
ORIGIN      
        1 atataatatg ttatattgct gatagaaaag agaataaaag aaaataggtt ttattaaaag
       61 agatttttat ttataggact taatcaatgt atagaggggt gagaacatat gtttaatgtt
      121 gcaattataa cacttagtga taaaggatat gaaggaaaaa gagaagatat aacaggaaaa
      181 aagttaactg aatttgttga aaatacagga gcttataaag ttactgaata tgtacttata
      241 aaagatgata aggaaatgct aaaggaaaat attataagac tttgtaatag taataaaata
      301 gatttaattc ttacaaatgg aggtactggt ttttcaaaaa gagatataac cccagaagca
      361 actaaggaag tgttggaaaa agaaatacca ggtctgagcg aatatatgag aatgaaatcc
      421 acagaaatta ctaaaaaggc catactaagt aggggtgtaa gtggaataag aaataattct
      481 ataataataa atttacctgg aagtcctaaa ggtgcagttg aaaatctaag ttttataatt
      541 gatgttttag accatggcat agaagtttta agaggagaag ctacagaatg tgccactaaa
      601 aaaagtgaat agtatataat ctagatagat aaaaaataga caaagaggta gataaagtat
      661 gattgataag tatggaagaa aaatagacta ccttcgtatt tcacttacag ataagtgtaa
      721 tttaaggtgt gtctattgta tgccaccaga tgtaaaattt gataaaaatt atataaatga
      781 aaatctaagc tttgaagact acaagtttat tataaaggct atggctgaac aaggtataac
      841 aaaaattaga tttactggag gagaacctct tttatatcct aaattaaatg agcttataaa
      901 atttacaaaa gaagaatgtg gaataaataa cataggaatg accactaatg ctattggtct
      961 ttctgataga atttttgagc ttgaaaaatg tggcttaaat aaagttaata taagtttaga
     1021 ttctttgaag gaatataagt ataaatccgt aacaagagga ggaaacttaa aagatgtctt
     1081 aaaatcgatt gaatcttgtt taaatttagg tataaaagta aagttaaatt gcgttgctat
     1141 aagtgggttt aatgatgatg agcttaaaga ttttatgttt ttaacagtta aatcaccaat
     1201 tgatgtaaga tttatagagc ttatgccatt aggtgaggct aatagagttt ataagaaggg
     1261 ttattttaat gtaaaagaaa tgattgaaaa aatatatggt ttatataaag tcaaaaatga
     1321 tgaaaaaagt acagcagaat attatatgtt taaaggagct aaaggaagaa taggaattat
     1381 aactccatta agctgttcat tttgtaacac ttgtaatcgt attagactta cttctagtgg
     1441 tgcaataaaa ttatgtctac attcaaaaga agaaatagat ataaaaccat ttttacataa
     1501 accattgtta tttagagaat ctatgaaaga tataataaaa caaaaaccag aaaaacatca
     1561 tttaatagaa aataaatgta gtgatacaaa taggcgtatg tatgaaattg gaggataagt
     1621 aatgttaaca catataaatg aaaaagggta tgccaagatg gtggacataa gtgaaaaaga
     1681 tgatactaaa agaacggctg tagctacagc tacaattact cttagtaagg aagcattaga
     1741 aaaagtaaaa aatggtcaga taaaaaaagg agacgtattg tctgtagctc aagttgcagg
     1801 tataatggga gctaaaaata catcttcaaa cataccaatg tgccatcaga ttaaattagt
     1861 tggatgtgat ttagaatttg atgtagatga ttctatgtct gctattaaga tatacgcaac
     1921 ctgtaaatct cttgggaaaa ctggagtaga aatggaagct ttaaatgcat gttctttagc
     1981 agcacttaca atttatgata tgtgtaaagc agttgataaa aaaatggtta taagtaatat
     2041 ccatcttgta gagaagactg gtggaaaatc aggagatttt aagttcgagt aaattaataa
     2101 tatattgggt taaaatttgg aggaaatatg gcgaaggtag tgtcaataaa tataagtgag
     2161 aaaaaaggtg taattaaagt tcctgtacaa tgtgcagagc taaaaataaa tcatgggata
     2221 gttggagatg cacatgcagg aaactggcat cgtcagataa gcttacttgg aaaagaaagc
     2281 atagataaaa tgaaaatgaa aggctttgac caattaaaat atggagattt tgctgaaaat
     2341 gtaactacag aagggataga agttttttcc ctgcctgttg gtacaaagtt aagaataggg
     2401 caatgtgaag ttgaagtaac tcaaataggg aaaaaatgtc acaatggatg tgaaattaag
     2461 aaattgacag gtgactgtgt gatgccacga gaaggaattt ttgtaaaggt tctaaaagaa
     2521 ggaattataa atgtgaatga ttcaatagat gtaatctaat gaagtcacta ttaaaatata
     2581 attataagta aatactatta agatttaaat atatatttta agagagaatt aattttaata
     2641 aaatatttta gttataaatt ggaatattgg ttatataaag ctatgaatgt aaaatcatag
     2701 ctttttgttt ttgaatttta aaatataata ttttatttat atatattaaa ttatatttta
     2761 tataaaatca attgtttaaa tatatattat ttatactgta aatttataga taataaagta
     2821 taaaaaaaat atattttgat gaaatttaaa aaaatacatt atataaatat agtaatagta
     2881 tgaaaattta catataaaac taaaaaatgc aagtaattga ctttttaagt tgacaaaata
     2941 ttgcatatat attatgatat aaatcataaa ttaactacaa atcaatatta tatattcgta
     3001 gaagaagaaa gtagcataaa ggaattttac agagagttag atttggctga gaactaatat
     3061 tggaatttat gtgaaaagga ctttggagaa tactattgta attatgttag aatagtggtt
     3121 taattcgcct taagaaaatg agtgagtaaa attaattttt actaataaga gtggtaacac
     3181 ggatttatat tcgtctcttg gatttttcca agaggcgttt tttattataa acttatttca
     3241 gaaaatttaa aactatatat taaatgaaaa ttataaaaat atgatagtta ataaatttat
     3301 aaattagaaa attgtaaaat atgctaaggt cagtagtttt acgatttaaa tggagggatg
     3361 aaaatggaag aagaaaaaat taactatgct ctggaacaga ttccagagaa tcaaaaacgt
     3421 ggttgggtag ctatgttttc agttttagta gcaataggtg tggatttatc atctgttatt
     3481 ttaggcgctg agttggcaca gagtatgcca atgaaacaag caatattgtc agtaattgtt
     3541 ggttcatttt tttctgcaat tttgtatact acatgctctt tggttggctc atctacaagt
     3601 ttatctacat ctatgataac aaagtatgta tttggagaag ctggagcaaa aatattttct
     3661 ctagttatag gggtttctct attaggttgg tttggagttc aagtaggttt ctttgctcaa
     3721 aatgcacaga taataataaa agatatattt aatttagatg taagtatgca gattttaagc
     3781 ctaataggtg gtctcttgat gatgagtact gcaatttatg gatataaggc aatggagaag
     3841 ttgagtgtgt attcagtacc atttttatta gttcttatga tgctaactat attcttagct
     3901 tttagagcaa atggaatttc agttgatgat aatatgaaat ctactatgac atttgcaggt
     3961 ggagtttctc tgtcaatgag tataataata gttggtgcaa ttgtatctcc tgatataagt
     4021 cgttgggcta aaagtagaag agactgtgct ctttcaagct ttttgggaat acaatttgga
     4081 aatgctttta tgataatagt ttcgatagta ttagtaaaat gtatgggaac ttcagatatt
     4141 atgagaattt ttattacatt aggaattgct ataccaggta taatagtact tacattagca
     4201 caatggacta caaatacaag taatgtatat tcagcatcat taagtatagc tcttgtactt
     4261 aagaaggctc ctgagaaagt attaactata gtccttggta ttatagcaac attattggct
     4321 gtatttggaa tatatgaagg ttttatagga tttttaaatt tacttggaat tgtaattgca
     4381 cctgttggtg gtgtatatac agcagaatat tacatagtaa agcaagaact taaaggcttt
     4441 gataaaggag tattatataa accaatagtt aaacgttcaa tagtgtcttg gataattggt
     4501 atattaataa cttacctttc aacatatgga tttataactc ttactactat agcaccttta
     4561 gatggtttta tagcagggtt tgtagtacaa tctattattg gaaaagtgct ttgttcaaca
     4621 aaaaataagc aagaaataga caaagcagta taaagaaatt tgtttataaa gtataaaata
     4681 aattagaaat atgaggagaa agtaacatga gatttttaac agaagaaagt gtagataaaa
     4741 tagcagttgg tgcagcagtg cttggaactg gaggtggagg tgacccatat gtaggtaaat
     4801 tggttgcaaa acaagccatt aaaaagtatg gtcctgtaaa agtaataagt ttagatgaac
     4861 ttgatgatga tgctttggta gttccagtat ctggtatggg ttctcctgta attacaatag
     4921 aaaaattact ttcagaagta gagttaacta cccctttaga aataatggag aaattattaa
     4981 atagaaaagt ggatgttata attcctatag aaataggagg gataaactca cttatgccta
     5041 tagcagttgc tgctaaaaaa ggacttccta tcttagatgc agattctatg ggtagagcgt
     5101 tccctgaagc tcaaatggta actttttatc ttgaaggata tggggcatct cctgccgtaa
     5161 tgtctgatga aaaaggtaat tctgccatcc tttatcctgt ggatggaatt tggtcagaaa
     5221 gattagctag aactttaact gtagaaatgg gaggaagttc ttctattagt gactataatt
     5281 taagtggagc tcaagttaaa aaagctgcta taggtaatac tcttacaata gctgaaacta
     5341 taggagggtt tttacttgaa aataaatctg attcaaaagg tgctgttgag aatatactaa
     5401 aagaattgcg tggatacaag ttatttgaag gaaaagtaat tgatattaag agagagttga
     5461 aaggtggatt tactagagga catgcctttt ttgctgggat taatgagtat gatggagagt
     5521 attctatatt attccaaaat gagaatttaa ttgccaaaaa aggtgataca ccactttgta
     5581 ttacaccaga tttaatagca gtattagatt tagaaactgg atttccaata actacagaga
     5641 gaataaaata tggctctcgt gttatggtag tagcttttcc ttgtaatgaa aagtggagaa
     5701 cagaaaaagg aattgagaca gttggacctg gatactttgg atatgatgtg gaatataaaa
     5761 cagtagaaga attacaagga aaataaaagt ttaggaggaa attttgatgt atcgtgtagg
     5821 aattgacgtt ggaggaacaa atacagatgc ttgtatactg gatggggaat taaaagtaat
     5881 tcattcagtt aaggtggcaa ctacaaaaga tgttgaaact ggtgtttaca atgccttaaa
     5941 aaaagttata gatgaaagta agattgacca ttcattaata aaatatgcta tgcttggtac
     6001 aactcattgt acaaatgcta ttgtagaaag aaaaagactt aataatgtgg ctatgataag
     6061 aataggaaag ccagccacta ctgccattct tccatttata gattggccag aagacttaag
     6121 agaaaaaata gaattagatt caatattggt atctggtgga tatgagtttg attaaaaaat
     6181 agctacgcac actcgtgact ttagtcatga gttagtagct aaattagtca gcatataggg
     6241 aaacctatat gtagagatag gatagaacct atccaatact acttgaatt
//
LOCUS       DAEMIK010000191         6272 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEMIK010000191 DAEMIK010000000
VERSION     DAEMIK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.194"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            156..1025
                     /gene="galU"
                     /locus_tag="KPK87_003469"
     CDS             156..1025
                     /gene="galU"
                     /locus_tag="KPK87_003469"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG2539575.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            1049..2062
                     /gene="galE"
                     /locus_tag="KPK87_003470"
     CDS             1049..2062
                     /gene="galE"
                     /locus_tag="KPK87_003470"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG2539576.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            2174..4108
                     /locus_tag="KPK87_003471"
     CDS             2174..4108
                     /locus_tag="KPK87_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG2539577.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            4263..5126
                     /locus_tag="KPK87_003472"
     CDS             4263..5126
                     /locus_tag="KPK87_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG2539578.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(5334..6215)
                     /locus_tag="KPK87_003473"
     CDS             complement(5334..6215)
                     /locus_tag="KPK87_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2539579.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLRERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
BASE COUNT         2481 a          713 c         1164 g         1914 t
ORIGIN      
        1 aacttataaa actaaaggta gatgaaattt catctacctt atattatctt atagcattaa
       61 atattgggat aatatagagt ttgaagaaaa agtataaata tggtatatta atttggtaca
      121 atagaaaatt tacgaagaag ggtgaaaaaa tgcagatgac agttaaaaaa gcaataatac
      181 cagcagcagg gctaggaact agatttttac cagcaacaaa atcacagccc aaagaaatgc
      241 ttcctatagt agataaacca actttacagt atataataga agaagctata gaatcgggaa
      301 ttgaagaaat tcttataata acaggtagaa ataaaaaatc tatagaagat cattttgata
      361 aatcagttga attagagtta gaactagagc aaaaaggtaa gacagagatg ttagaaatgg
      421 taagagacat atcaaatatg gtaaatattc attatataag acaaaaagag ccaaaaggtc
      481 ttggacatgc aatttactgt gcaaaaagtt ttataggtga tgaacctttt gccgtattat
      541 taggtgatga tatagtagat tcagatgttc cgtgcttaaa acaattaata gatacttata
      601 atgagtataa aacaactgta ttaggtgttc aaaagatagc taaagaagat actaataaat
      661 atggtatatt ggatgtaaaa catatcgaag atagagtata taaagttaaa gatatggttg
      721 aaaaacctgc aattgaagaa gctccatctg atatagctat acttggaaga tatataataa
      781 ctcctgcaat atttagcata ttagagaaac agacacctgg taaaggtgga gagatacaac
      841 tgacagatgc acttcaaact cttggaaaac aagaggctat ttatgcttat aactttgaag
      901 gtagaagata tgatgtggga gataagattg gttttttaga ggcaacaata gactttgctc
      961 taaagagaga aaatttaaaa gatgatttaa tggactatat gagaaaaaaa gtaagtgaaa
     1021 aataaatatt agaaaagagg taattacaat ggcagtttta gtagctggag gagcaggata
     1081 tataggaagc catacagcaa tagaactttt agaatcaggt tatgaagttg ttatagttga
     1141 taacttaagt aatagcaact caatagtggt tgatagaatc aaggaattat ctaaaaaacc
     1201 agtaaaattt tataatattg atatcagaaa taaagatgaa atgcatgttg tatttaaaga
     1261 aaataatatt gaatcaatta tacactttgc agctttaaaa gcagttggtg aatcagtaga
     1321 aaaaccaatt gaatattaca gcaataatct aataagtacc ttaaatttat ttgaattaat
     1381 gagagaatat ggtgttaaaa agtttgtatt tagttcttca gctactgtat atggagaccc
     1441 acatacttgt cctatattag aggatttccc actttctgta accaatccat atggaagaac
     1501 taaacttatg atagagcaga tgttagtaga tatatcaaaa gcagataagt cattggatat
     1561 agctctttta agatacttta atccagtcgg tgctcataaa agtggtagaa taggtgaaga
     1621 accaaatgga gttccgagca atctaatgcc ttatataaca aaaatagcag taggtaaatt
     1681 gaaggaattg agtgtttatg gtaatgacta tccaacacat gatggaactg gagttagaga
     1741 ctatatacat gtattagatt tagctgctgg tcatgtaaaa gcattacaaa aattagaaga
     1801 aaatccagga cttgttgttt ataatttagg aactggaaag gggtatagtg ttcttgattt
     1861 agtaaaagca ttttctaaag caagtggaaa agaaatacca tataagattg taggaagaag
     1921 ggctggagat gtagccatgt gttatgcaga ttcttcaaag gcagaaaaag agcttggatg
     1981 gaaagctaag tatgaacttg aagagatgtg tgaagattca tggagatggc aaagtatgaa
     2041 tcctaatggg tatgaagagt agaatacata cttatatgta taaatgtaac aaatttgtaa
     2101 atataaggta ttggtaattt tatgttataa ttagacacat gtagtaatgt ttctaaaaga
     2161 gggggaagtt atagtgaaaa aaagtctaaa aaagtattta gtactagcac tgactttagt
     2221 tttagtattt gcatgtggcg taccagaatc aagcgcagcc agtaaacatg tgattatagt
     2281 aaattcaaga aaaaatactc taggatattt tgtaaataat aaacttgtaa aagaatttag
     2341 agttgcaaca ggtaaaaagg ggtcagaaac accaacaggg aaaacaaaag tagttaacaa
     2401 gataaaaaat agaccatatt ataaaggtaa tatacctgga ggaagtccaa gaaatccatt
     2461 aggagataga tggatgggat tagcattaaa aggaacttat ggagatacat atggaataca
     2521 tggaaataat aatgagagtt ctataggaaa gcatatttct ggtggatgta taagaatgca
     2581 caataaagat gttagatggt tatttgacca agttcctgta ggttcagatg taattataga
     2641 ttatagtaac gacagctatg taaagatagc ggcaaaatat aagataaatc taaatcaaac
     2701 aggatggaaa acagaaaatg gtaagaagta ttatgtaaaa tctgatggaa cataccaaaa
     2761 gaatagttgg ctaaaagtaa atggaaaaat gtattacttt gatgcttcag gagtaatgca
     2821 aacaggatgg aaaacaatta ataacaaaaa atattattta ggaactgatg gagcaagagt
     2881 atctggatgg aaagtaatag acggaaaaac ttattacttt aactcagatg gagtaatgca
     2941 aacaggatgg caagaaaaga atggaaagaa atactatcta ggatctgatg gtttagcagt
     3001 aacaggatgg caagaaatag atggtaataa gtattacttt gacaaaacag gaataatgca
     3061 gacaggatgg caacaaatag atggtaagtc ttactattta gacaaagatg gaaagatgct
     3121 aactggttct caaaaaatag atgggaaaga ttatactttt aatgaagatg gaactataaa
     3181 tcctacatgg gatacaatta taggtgcaaa tagatttgat acagctaaaa agataagttc
     3241 agttggaaac tggaatgctg atagctcaga tactgtaata ttagtaaatg gtaatgcaat
     3301 agctgatggt ataacagcaa ctcctttagc aagtagctat gactcaacaa tactacttac
     3361 taatacagct aatttaccaa ctgaaactgt tgaagaaatg aagttattag cacctaaaac
     3421 agtaatcttg ataggtggag aaaatgcaat aagttctaaa ttagagcaag aaataaaaac
     3481 tactttcaac acagaaacaa agagaatagc aggtcaagat agatatcaaa ctgctacaag
     3541 aatagctgaa gaacttggaa atcgagaaga aataaaaact gcatatatgg tttctggaaa
     3601 tggggaggca gatgctttat ctgtagcttc taaagctggg gaagaaaaac aacctataat
     3661 attagtcaat aaagatggaa ttacagaaga atcttataaa tggctaacag aaagaaaatt
     3721 agaaaatgca tactttatag gtggaccaag tgcaattaac gattcagtaa tagcaaaaat
     3781 gaatgatata actactgaag atatttcagg aaatagaata tatggagata gtagagtaga
     3841 tactaatgcc aaagtaatag aaaaattcta tggagatact gatttacagg ctgtattagt
     3901 tagtaaatca gatgctttag tagatgcttt atcagctgga ccattagctg taaaattaca
     3961 ttcaccaata gttttaatgg ataattcagg attatcatca gagcaacaaa gagtatttgc
     4021 aaataaaaaa gttgaaactc cttatcaaat aggaggcgga gtatcttata tagtaatgga
     4081 taaattaatg gatattcttg caaaatagta aaaatatata ttgaaaaata attataattg
     4141 agatgtgaaa ttcaatcaat aaaagcttga ttgttttaca tctcaatttt ttagtataaa
     4201 attaagttta agttatataa aaatcttgga atatttttaa aactcatata aaatgaatat
     4261 agatgctatc atcatatgtt aagagggtga gtaagattat aaagaaaaaa tatataatgt
     4321 ttattgatga aagtggaaga gctgatataa atcatgaaga cccatttaca ttgacaggag
     4381 taatttttga atataaatat gcagtaagtc aaggcgatta tgtatgttct ttgaggaaag
     4441 aaatggatgc atttaaagag tattgttttg gaaccagtaa cataggaata catttgacag
     4501 atatatcaag aaaatcaaga gcatttgaga agtttgatga ttcacagatt aagttattct
     4561 atgatgagtt gcctttattt ctatctagat tagagtgtac tataatatca gtaacagtag
     4621 ataaagctag attaaagaaa tattatgcac cttcaaagga accgtatgtt gtggctttta
     4681 tacatgttct tcagaatttt tattcatttg taaataatga aactgtagaa tctgcacgaa
     4741 tagttataga gtcaagggat gatgtctcaa acttaaaagt acaaaaggcg ttttttgatg
     4801 tatttaataa tggaacaata tacttagata tagatgaaga attaagagat aaggttaagg
     4861 gttttataat tgccaaaaaa gaagatgctg actatagaga aggtttagaa atagcagata
     4921 ttttatgtaa tcctcttagc agagcaagaa gagggttaat agaagctaat ccaaagtgta
     4981 tgcaatatgg agaaaaaaac aaaatattca aatctgtcag aaacaaaata tatactccaa
     5041 cgagtgtgaa tgatataaga aattggggat ttaaagaagt tcctattgta gagcctgttg
     5101 gaccatggct tgaaaaaatg cgttagatac aattgacaaa ttttaaaaaa tctgatataa
     5161 tgtaagtaac ctagttattc aggaactgaa tccagttgct gttgtataac gataattact
     5221 gagactcgga tgccgactgt cagttaggaa tctatacttt taaaagtata gattttttta
     5281 ttttataaaa agaataaaag aggatattta aaaataaccc ctttattaat aaattataat
     5341 tgagcgtttt taatgacaaa gtttttaaag tttttaattg caggaggcaa atatctatct
     5401 tttataaatg ctaagtaaaa gtttctttcc caacttgggt gaataatctg aattactttt
     5461 acttttgtaa agctaagcat attcatattt gggactacag caattccgaa attttttgca
     5521 actaatccag caattacttg gtcttcctct acttcataga ctatattagg ctgttgattt
     5581 atttttttaa acatatcatc aattatatat ctaagaccgc ttctctgatt aaatactatt
     5641 tgaggataag gtatggtttc ttttaaatct atagtatcct ttgcagctag tggatgacta
     5701 tatggtacaa ttagtactaa atcttgtttg gcaacaggta taaattcaat tcccttttct
     5761 ttttcaagtt tcgaacaaaa tgcaatatca tattttcttt ctctaagacc ttgaataatg
     5821 tcagtagtta caccagtatg aaatttaaaa tctattgatt tagcaggatt agattttaaa
     5881 aatttatgca ctatgtctgg gataaaatca gtaccaagtg ttctaagaaa agctaagtct
     5941 atttgtcctt caccagtacc agttattttt aatgatttta cacttgaatc taaaatttct
     6001 aatgattttt ccacatcttt taaaaatatt tttccatact tagtaagaac tatatttcta
     6061 ccttcttttt caaatagagc caccccgagt tcattttcta gtaaagaaat agcatgactt
     6121 aagcttggtt gtgttataga taaattttct gccgcttttg tatagtgttc taaatgtgcc
     6181 aatgtaacaa aatagcgtaa gtgatataaa ttcaattttt gttcatcctt tcataggtat
     6241 ttttatttat atatttgtag aagttaacct tt
//
LOCUS       DAEMIK010000192         6109 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEMIK010000192 DAEMIK010000000
VERSION     DAEMIK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.195"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            225..2933
                     /gene="hpdB"
                     /locus_tag="KPK87_003474"
     CDS             225..2933
                     /gene="hpdB"
                     /locus_tag="KPK87_003474"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG2539580.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            2937..3194
                     /gene="hpdC"
                     /locus_tag="KPK87_003475"
     CDS             2937..3194
                     /gene="hpdC"
                     /locus_tag="KPK87_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG2539581.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            3187..4137
                     /gene="hpdA"
                     /locus_tag="KPK87_003476"
     CDS             3187..4137
                     /gene="hpdA"
                     /locus_tag="KPK87_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG2539582.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWTQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            4452..5414
                     /locus_tag="KPK87_003477"
     CDS             4452..5414
                     /locus_tag="KPK87_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2539583.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMTTFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(5513..>6109)
                     /locus_tag="KPK87_003478"
     CDS             complement(5513..>6109)
                     /locus_tag="KPK87_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2539584.1"
                     /translation="IIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVPTSFILILQGAQ
                     PNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSLW
                     HLPLIFTLYTPEVPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLI
                     TNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         2270 a          677 c         1114 g         2048 t
ORIGIN      
        1 aataaatata gtatatatta aaacttgaag taaaactatt tataatcttt atattccaat
       61 gaattgcaac aaattttagg atatacagat tgtctttggc ttagatttgc ttcaagttta
      121 attttttcta tagaaagatt attttaaaaa gtgggtaata tacccttttt tttcattctt
      181 aaaaagtttt atactaatta tagaaagatt aaggagggga agaaatgagt caaagtaaag
      241 aagacaaaat aagaagtatt ttggaagcaa aaaatattaa atcaaatttt caaaataaag
      301 aaaatttaag tgaatttaat gaaaaaaaag caagtaaaag agcagaagac ttattagatg
      361 tatattataa tacattatct actgctgata tggaatttcc atattggtat aatagagaat
      421 atagaaaatc agatggtgat ataccagtag ttagaagagc taaagctctt aaagctgctt
      481 tttcacatat gacaccaaat ataatacctg gtgagaaaat agtaatgcaa aagacacgcc
      541 attatagagg ttcttttcca atgccatggg taagtgaaag cttctttgtt gctcaaggag
      601 aacaaatgag agaagaggca aagaaattag ccagtaacac agctgatgaa cttacaaaat
      661 ttgggtctgg tggaggaaat gttactgaaa gttttggaaa tgtcgtttca atagctggta
      721 aattcggaat gagaaaagaa gaagtacctg tacttgtaaa aatggcaaag gaatgggttg
      781 gaaagtctgt tgaagattta ggttttcact atgaaaaaat gatgccagat tatgatttaa
      841 aagaaaacct aatgagtaca cttatatgca tgtttgattc agggtataca cttcctcaag
      901 gtagagaagt tataaattat ttttatcctt taaattatgg attagatgga attatagaaa
      961 tggcaaaaga atgtaaaaag gcagttgcag gaaatgcatc tggagatggg cttattggta
     1021 tggaccgtct atatttttat gaggctgtta ttcaagttat tgaaggatta caaacttgga
     1081 ttttaaatta tgcaaaacat gcaaaatatt tagaaagtat agagacagat ttagaagcta
     1141 aaaaagaata ctcagattta gtggagatat tagaacatat tgcacataaa caacctcgta
     1201 catttaggga agctcttcag ttgacttata caattcatat agcatcagta aatgaggatg
     1261 ctatatctgg tatgtctata ggacgttttg gtcaaatttt atatccttgg tatgaacaag
     1321 atatagaaaa aggactaatt acaaaagaag aggttatcga attattagag ctttatagaa
     1381 taaaaatcac ttgtattgac tgttttgctt cagcgggcgt aaatggagga gtactatcag
     1441 gcaatacatt taatacttta tctataggtg gattaaaaga agatggttct acaggagcta
     1501 atgaactaga agagttatta ttagaagcaa gtatgagatg tagaactcct caacctagtt
     1561 taactatgct atatgatgaa aagcttccag aagatttctt aatgaaagct gctgaatgta
     1621 caaagcttgg ttctggttat ccagcatggg taaacaactc aaatggaaca acatttatga
     1681 tgaaacaatt tgctgatgaa ggtatgactg ttgaagaagc aagagcattt gcattaggtg
     1741 gatgtttgga aacgtctcca gggtgttgga aacagttaac tttaaacgga aaaacatact
     1801 ctattgcagg tggagcaggt caatctgctg gctctggagt tcactttata gctaatccaa
     1861 aaattttaga acttgttctt atgaatggta aagactatcg tatgaatata caagtatttg
     1921 agcctcataa taagccatta gatacttatg aagaagtcat agaagtattt aaagattatt
     1981 ataaacaagc aataaatgtt ttagaaagag caaataacat tgagttagat atatggagaa
     2041 aatttgatac ttctataata aattcactac taaagccaga ctgtttagac aaaggtcaac
     2101 atataggaaa tatgggatat cgttataatg caacattaaa cgtagaaact tgtggtactg
     2161 ttactatggt caattcattt gcagctctta agaaacttgt atatgatgac aaagcattta
     2221 caatagagga aatgaaggat gccatattaa ataattttgg atttaaagat gcattagaag
     2281 taggaaatta ttctatggca gaccaagtaa aagtagataa gactggtaaa tatgatgcta
     2341 tttataaggc ctgtcttgat gcacctaaat atggaaataa tgatttgtat gcagataata
     2401 ttcttaaaaa ctatgaggta tggttatcaa aagtatgtga agaagcacaa tctttatatg
     2461 ctaagaagat gtacccttgc caaatatctg tttctacaca tggtccacag ggagctgcta
     2521 ccttggctac tccagatggt agactaagtg gaacaacata ttcagatggt tcagtctctg
     2581 catatgcagg aacagataaa aatggagtat atgcattatt tgaatctgca actatttggg
     2641 accaagcagt agttcaaaat tctcagatga atttaaaact tcatccaact actataaaag
     2701 gacaacaagg aactaaaaaa ttattagatt taacaagaag ttatttaaga aaaggtgggt
     2761 tccatattca atataatgtt gtagattcag aaacattaaa agatgcacaa aaaaatcctg
     2821 acaattatcg tcaattaatg gtacgtgttg ctgggtttac tcaatattgg tgtgaattag
     2881 gtaaaccaat acaagatgaa gtaattgcta gaacagagta tgaaggggtg taaaatatga
     2941 gaaagcatag tgattgtatg aatttttgtg cagtagatgc aaccaaagga atttgtagat
     3001 tatcaaaaca aatgattaat ttagatgatg cagcatgtcc agagataaaa gtaatgccaa
     3061 aatgtaaaaa ttgtaaaaat tttgttgaag ctaatgatga aggtataggc aaatgtgttg
     3121 gcctagagaa agaagactgg gtatattcaa cattgaatgc aattacttgt gaaggacatg
     3181 tgtttaatga gtagtcaaaa gcaattagaa ggcatgattt ttgatgtaca aagcttttca
     3241 gttcatgatg gtccaggttg tagaacaact gtatttttaa atggatgtcc actgagttgt
     3301 aaatggtgtg caaatccaga aagttggact gtaagaccac atatgatgtt tagtgaatta
     3361 tcttgtcaat atgaaaatgg atgtactgta tgtcatggta agtgtaaaaa tggtgcattg
     3421 agctttaatc ttgataataa gccagttata gattggaaca tctgtaagga ctgtgaaagt
     3481 tttgaatgcg tcaactcatg ttattataat gcgtttaaat tatgtgcaaa accttacact
     3541 gtagatgaac ttgtgcaggt aattaaacgt gattctaaca attggagaag taatggagga
     3601 gtaacattta gtggaggtga gccactactt cagcatgaat ttttacatga agtattatta
     3661 aagtgtcatg aggtaaatat acacactgcc attgaaacga gtgcatgtgt atcaaatgaa
     3721 gtttttaata aaatatttaa cgatattgat tttgcattta ttgatataaa gcatatggat
     3781 agagaaaaac acaaagaaca aacaggtgtg tataatgatt taatattaga aaatatatca
     3841 aaccttgcaa attctgattg gaatggaaga ttagtactta gagtaccagt tatctctggg
     3901 tttaatgata gtgatgaaaa tataagtgat attatatctt ttatgcacaa aaataatttg
     3961 gtagaaatta atctattacc atttcacaga ttaggagaat caaaatggac tcaattaggc
     4021 aaggaatatg agtattctga taagggtgat gttgatgaag gtcatcttga agaattacaa
     4081 gatatatttt tggataatgg tatagcttgt tatgttggtc atgagacagc tttctaaaat
     4141 taaatacaag ttttaattaa aaaaggatgt tacactaaaa gctagagtta catccttttt
     4201 tatgtggttt ttatgtagaa ataattaagt aaagttttat atgttttata tattgtttat
     4261 attgctttat gtccataaat attgctttaa atatacttta tatatttttt atagtttaaa
     4321 ttacagttag agaatatatt acgtttactt cattgaagca gaagttttgt ggtaaaatac
     4381 tactatgaaa aaatttaata gaatatattt tagaaacaac taaaaataat agttaggagg
     4441 aagacatcta tatgaacaaa tcatggagca acttgattat taagaataaa gacagaaaag
     4501 tacgttcagg gtggattatt atagctgtta tgactacctt ttacatactt cagtattatt
     4561 taagcatgat acttgtggaa ataataagaa aaatcctggt tgctacaggt gacattaatt
     4621 taaaaacaaa ctatttttca gaatatgcta attggttaaa tgatgttggg ttacagattg
     4681 tatttcaaat tctaacagat tcacttatga ttattattcc tataattgta tggagattta
     4741 ttatgaaaaa tcctattagt gaaatgggat tacattctta tagaattaag aaaaaagaag
     4801 gatatgtagg aatgttatta ggaatattta attgtacagt aatctttatt ataacaataa
     4861 caattggtgg tggtcatgtt acttcatggg taccaaaaat ttcaccttta acagtttctt
     4921 ggatacttgt ctttatttta gttgcttttg cagaagaaat tttaaatcgt ggttttttta
     4981 tggcagtttt acgaagatgt aaaaatattt attttataat gattgttcca tctgttattt
     5041 ttgggttgat tcatatatgg aatcctgatg ttactttttt gtctgtaatt aatattataa
     5101 ttattgggat tcttttttca tatatgttta taaagtcgtc gaatatatgg atgtgtatag
     5161 gttatcattt tacatggaat gtatttcagg ggattatata tggtatgcct gtaagtggac
     5221 ttcaggttcc agggattata actacacaaa tcactcatgg taatctatta aatggtggta
     5281 tgtttggtat tgaaggtgga attttgacta cttttgttaa tctacttagc tttatatttg
     5341 tatggtatta ctatagagat tctaagtatg attttgttag tgataaagta aatgtgacca
     5401 atatcaagaa ttagttgatt ataataaatt ttaataatat cttctaagct taaagtattc
     5461 tgtaggattg ttggaaattt acaaaaatat aaaaataact tggatattct atttactcca
     5521 agttattttt atattctttt gtaaatagaa atggaacata aaaaattaaa acaaccatag
     5581 atactattag tatatcatgg taagtgatag ttgattcttt agaatttgtg ataagaaaag
     5641 cactattatt taaaacatga attatagcac aaaaccagat attcttagtt tttatataaa
     5701 gatatcctaa aaatattgaa attccaacta tatgtattga acgtaatatt attcctaata
     5761 ttggcacttc aggtgtatat aatgtaaata ttagtggtaa gtgccacaag ctccaacaca
     5821 tacctaatat tataactcct aactttttac caaatttatc aaaaaaaata ttctgtaaaa
     5881 aacctctcca ggcatattct tcactaaaaa aaatataaat agtggttaat gatgttattg
     5941 gagtaaatat aaattttatg ataccactat aattaggttg agccccttgt agtattagta
     6001 taaatgatgt tggaacaatt acagaaatca taaagtaaat aattaataaa gaacctagtt
     6061 taatattttt tacaggatat aaattttttt tatttgatga atatattat
//
LOCUS       DAEMIK010000193         6086 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEMIK010000193 DAEMIK010000000
VERSION     DAEMIK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.196"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(503..1024)
                     /locus_tag="KPK87_003479"
     CDS             complement(503..1024)
                     /locus_tag="KPK87_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539585.1"
                     /translation="MKFKRLRTSENKLYYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            1380..2176
                     /locus_tag="KPK87_003480"
                     /pseudo
     CDS             1380..2176
                     /locus_tag="KPK87_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            2286..2876
                     /locus_tag="KPK87_003481"
     CDS             2286..2876
                     /locus_tag="KPK87_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG2539586.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMMHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            2942..4639
                     /locus_tag="KPK87_003482"
     CDS             2942..4639
                     /locus_tag="KPK87_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG2539587.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIKGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            4853..5692
                     /locus_tag="KPK87_003483"
     CDS             4853..5692
                     /locus_tag="KPK87_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG2539588.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         2408 a          610 c          990 g         2078 t
ORIGIN      
        1 aatgagctta gagaagaacc aatttttaag gatgtaattg aaaagatttt agatatggaa
       61 aaaatgttga aaaataaaga ataagtaaaa atttaatata ggacttaaag attaatcaaa
      121 ttaaaagaaa gtgtttcaaa ataaaaattt gaatcacctt ttttgtctgt cttaaacagt
      181 tatttagaaa aatagaggtt tcaaaataga aatctgaatt atctgtatta ttacacaaaa
      241 atagaaacct aaatttataa aaatagaata aagtttgtta tataaaatgt aattttgtct
      301 ataaaatctc acctgttttc taactttaag cgtgaaaaag agaatgtatc atgaattttt
      361 tagttcatga tgcatccttc tttgtatgaa atccaatata tctttattat ttaaacaatg
      421 gaaaagagtc ttatttctat tttgagacat ctcttttatt taattatgaa gttttataca
      481 gttcaataaa taggaattat atttagaaac tcataacatt atttcttaga tagtgattaa
      541 atttatcata ttccatttta gataactttt ctgggtaaga cattacaatc aggttatgcc
      601 cattaaattt ttcatggtat ggtggatgaa tgtgttcata attatttgtc ttatatttta
      661 ctctttcata gaacgccttg cgataaattg aaatctcatc aattggaagg tcaatttcta
      721 aaataatagt ttttcctttc ttatttaaaa gctctaacgc tctttgtcca tatctattat
      781 tgcgcatttc tggaaaaatg cagaaatgtt ctacatagat aaagtcatct gtttcccaac
      841 ataaaattat gccgacaaac tggtttttat cataaattaa attgaattga tattctttat
      901 ttttcaaaat ttcttcttgc aaagaagatt ttctttgttc atgaaatggg aaactaattt
      961 tatataattc catagccttg taatataatt tattttcact ggttcttaac cgtttaaatt
     1021 tcactttact cctcctaatc actaaaattc caatttattt atgagttcat tttaccatag
     1081 tcagtatttg catacaatgt gttttaagtt gagtgtcatt tctttctttt atttatgttt
     1141 atatctcata gactaagaga taattgctta agacataact aaaaactatt atattaagct
     1201 ttgataatct aaaaacagta tatttatgga cttttctagg tctaaaagta atcttaataa
     1261 ttttagaagt tacaaaaaag ttacataaat ttggaaagct ataatataca tatataatat
     1321 aaggttaaaa ctgtaatgaa taatattaat ttaaaatttg aacatggaag gagattatta
     1381 tggagaaaag agaaaagagg ataataatat catctttatt aagtgtatca attttaatgg
     1441 gtttgacaag tatatattct atattaaata aagaagacat aattttaact gttaagggtc
     1501 aagagcaaaa agtatcttcc tttaagaaaa cagttgagga acttttggat gaacagggtg
     1561 taaagtataa ttctgaagat aagattaatc caagtttaga tacagaacta aaagatgata
     1621 tgaaaataaa agttgttaag gtaactaaaa gtaaaaaaga agagattgaa aaaattccat
     1681 ttgatacaaa gcatgtaaat gatagtaatt tgttaaaagg aaaatctaaa gtttatcaag
     1741 aaggtcaaga aggagaaaaa aactagtcta taatttaact taccatgatg gaaagttagt
     1801 caaaaaagtc ttatcaaaag aagtaatatc taaggagcca actacaaaaa ttataaaata
     1861 tggaactaaa gaaaaagtac taatagcatc aagaggagca aatataagag gaggcaaaca
     1921 tatgaaagtg gttgccactg catatgcagg agatacaata acatctactg gtacaactcc
     1981 aagatggggt gttattgcag ttgacccacg tgtaatacca tatggaacaa aagtatatat
     2041 acctaaactg ggtatgactt ttgtagcaga agattgtgga ggtgcaatta aaggtaatag
     2101 aatagacatt tttatgaaca gtgaaggaaa agcttcaaat tggggaagga aaagtataga
     2161 tatatatcta cattaaatta gtgaatagaa taactttact tgaaggacaa aaaagtataa
     2221 aatatgaatt aactaaactg ttagattatt cttaaaacat aaaaatatat ctaagggggg
     2281 ataagatgaa aatagtctgc ttaggagaca gtttaacgta tggatttgga gtatcacgaa
     2341 gtaattcatg gacaaacatt gtaaataaag agactcgatt agaaatagta aataagggta
     2401 taaatggtga tacaacaagt ggtatgttag ctaggtttaa tgaagatgtt gtaaaaaatt
     2461 ctcctgatat agtttttata atgggtggca ccaatgattt tattgctggt gcgggaaatg
     2521 aaattattaa ttctaatata atggctatga tgcatcagtc atattcaaaa aatattatac
     2581 ctataatagg tataccatta aaaccagata ttccaaatgt tagggaagat tggagttgtt
     2641 ttactgattt taatatagta tctcaaaact tagaatcata tagttattgg ataaaaaaat
     2701 tttgtatgac atttaatact aatttcgtag atttttattc agaatataat aaaaatatgg
     2761 aactagaagg atataaaaaa ttatattttg atgggttaca tccaaccaaa gagggtcata
     2821 gaataatggc agacattttt attaattcaa ttaataagta tatagaagag ttatagatta
     2881 cattaaaatg cttgtttatg cgtcagttgc aataaataca tacagtagag aggggtttat
     2941 tatggattat actaaaactt atgaagagtg gataaagggt tcatattttg atgaagatac
     3001 aaagctagag ttggagaata taaaaaataa tgaaaaagaa atagaggata gattctataa
     3061 ggatttagaa tttgggactg cgggtcttag aggaataatt gaagcaggaa cgaatagaat
     3121 aaataagtac acagttagaa gagctacttt tggattggca aactatatat tagaaaacac
     3181 aacaaaagaa gaaacaagta gaggagtagt aatagctcat gataatagac acaagtctag
     3241 acaattctgt atagaatctg caaatacttt ggcagcatgt ggtataaaag catatatatt
     3301 tgatagtttg agaactactc cagaattatc ttttgctgtt agaagcttaa atgcaatcgc
     3361 tggtatagtc ataacagcaa gccataatcc tccagaatat aatggatata aggtatactg
     3421 ggaagatgga gcacaagtta tgccagagat tgcaaatgca ataacagaga aagtaaatag
     3481 tatacatgat tatagtacaa taccaacctt aacggaagct aataaaaatt tggtagtttt
     3541 gttggacgaa tcacaagata ctaaatttat agaagcagta aaaagccaaa ttataagaaa
     3601 agatttagtt aaaaatgtag gtaaaagctt taagatagtt tatacacctc tttgtggaac
     3661 agggaatgtt ccaataagaa gagcattgaa agaagttggt tttgaaaata taattgtagt
     3721 tccagaagaa gaaaatccag acccaaattt tgcagggctt gattatccaa atccagaaga
     3781 gaaaaaagct ttaaatagag gtatattact cgcaaaagaa aaaggagcag accttgtaat
     3841 agcaactgac ccagattgtg atagagtagg tgtagcagta aaaactacta caggtgagta
     3901 tgctttactt acaggaaatc aaattggtgg tatgctgaca cattatataa taaaaggatt
     3961 aaaagaaaat aacaaattaa aagaaaatcc tacaataata aaaaccatag ttacatcaga
     4021 atttggagca gatatagcta aagcaaataa tgttgatgta ttaaatgttc ttacaggttt
     4081 taaatttatt ggtgaaaaaa taaaattatt tgaacaaaat aaaaatagaa gttatgtatt
     4141 tggatatgaa gaaagttatg gttacttagt tgggactcat gctagagata aagatggtgt
     4201 agtttcttca ttgttaatat ctgaaatggc agcattttac tattcaaaag gtatgagttt
     4261 atatgatggt ttaatagaac tttataaaaa gtatggattt ttcaaagaac agactatatc
     4321 tttgacttta aaaggtatag aaggtgtaga aaagataaaa gaaataatat cttattttag
     4381 agaaaatcaa attgattata taaatagtat taaagtagtt gataaaaaag attataaaaa
     4441 tggtatagat aatcttccta aatctgatgt tttaaaatac tttttggaag atgaatcttg
     4501 ggtagctata agaccatctg gaacagaacc aaaacttaaa ttttacatag ctgtaaaagg
     4561 agctagtgat attgaagcag ataaaaagat tcaaggtttg aaaaagtata tagatgacat
     4621 ggtcgaaaaa ttaaaataaa atattattat tttataagat aattttgtaa taatatttat
     4681 ttagctgaaa attataattt actttcattg agtagaatat ggaaatgtta gtagaattgt
     4741 tattttcagc ttttatttgt gttattttta cttaaataat tatattatag aatgaatctg
     4801 agtataatta atattgtata atgaaattaa tttgatagaa aaggggatat ttatgtttgg
     4861 tatagataag aaattgattg aatatataat tgcaaaatcc aattacagtg aattaagttc
     4921 aaagtcagca gaagtatctt tcttcttaat gttgtccata tttccttttt taatatttac
     4981 aataagttct atagcttata taccgattct tcatttaaat aagtatatag ctttgtttag
     5041 aaatatgatg ccagaaggtg cttttgctgt gttatcttca atcatagttt ctgcaataga
     5101 caatagaaac ctgaaatttt tagcagtcag ttttgtactt actatgtgga cattttcaag
     5161 agcagtaaaa gctcttatta aaggaatgaa tagagcctat aaagttaaag aaactaggtc
     5221 tttttttaaa attttatcta tatcattttt atttacaata atgcttttgg tccttatttt
     5281 tttatctatg atatttttag tttacgggga aaaaataggt tattttattt ttaatcttgt
     5341 aggattggat gagatattta taaaaatatg ggatattcta agatacactg taggtataat
     5401 aacaataata gtgatattta cacttttata taaatacact ccaaataaaa agttaactat
     5461 taaagaatct gcgccaggtg ctatttttgc tacttttgct tggtttttag tatcattttt
     5521 atattcttat tatacaaatt actatgctaa ctatgaagta atctatggaa gtatagctga
     5581 aataattgta cttatgacct ggatgtattt tagtagttgg tctatcgtta ttggctatga
     5641 ggtaaattca agattgtatt ttagaaagat aagacatgaa atgttaaagt aaagtattta
     5701 aaataaaaga gagtatttag tattataaat gtaattgaat taataaagaa atattgacat
     5761 ttaagttaaa aagtctagaa tttttctggg ctttttttat ttttagtata acttgacaaa
     5821 ttttatttaa tgggatagtt ttaatttata atcatcttta aaaagatgac tattgataag
     5881 taagagataa aattggtaag tatcaaaaaa tatactaaat aaaaattata gtcctaatga
     5941 gttaatttct gtattttcaa ataatgcagt atttagtcat gtgtttttat atgtgcctgt
     6001 atttctaatt accaatatat tagaataagc tactgtaatt aggtttagaa aaaagcgaca
     6061 taaataatta taaacaaata ataaat
//
LOCUS       DAEMIK010000194         6071 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEMIK010000194 DAEMIK010000000
VERSION     DAEMIK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.197"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            232..1044
                     /gene="modA"
                     /locus_tag="KPK87_003484"
     CDS             232..1044
                     /gene="modA"
                     /locus_tag="KPK87_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2539589.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1054..1845
                     /locus_tag="KPK87_003485"
     CDS             1054..1845
                     /locus_tag="KPK87_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539590.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1847..2542
                     /locus_tag="KPK87_003486"
     CDS             1847..2542
                     /locus_tag="KPK87_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2539591.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAKPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2529..3260
                     /locus_tag="KPK87_003487"
     CDS             2529..3260
                     /locus_tag="KPK87_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539592.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3574..4374
                     /locus_tag="KPK87_003488"
     CDS             3574..4374
                     /locus_tag="KPK87_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2539593.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNRLGVIRENIILLPETNTNGNTGFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPSILHTPNL"
     gene            4914..5630
                     /locus_tag="KPK87_003489"
     CDS             4914..5630
                     /locus_tag="KPK87_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539594.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2376 a          732 c          845 g         2118 t
ORIGIN      
        1 caataagtta atttgattta agtttttaaa tttaaaatac tttaaagtat taataaaaat
       61 taaaaataaa ataactacct atggaaatga cattataaat agattttttc ttaactatac
      121 ataacaaaac ataacaaaac attataaaac ataacaaaaa cttagacatc gttttcttag
      181 tatgatatac taaaaaagaa tttacactaa tttactaagg gggaatatct tatgtttaaa
      241 aaactttcag tactagcttt agctatgatt atttcattaa gtattactgc atgtaattct
      301 aaagaaaaca aagctaatga tgaatctaat actaaagtga caacagctga tgattcaaaa
      361 gaaaaagaga ctctttttgt ctattcaggg gcaggtttaa agaaaccaat ggatgaaata
      421 gcaaaaaaat ttgagaaaga aaataatgtc aaagttgaat atagctatgc aggttctgca
      481 cagctaatag ctcaaattga gacaagtcac aaaggagacg tatttattgt tggttctgaa
      541 ccaatttatt caaaggcctt tgaaaaagaa cttgtaggtg aacataaaac ggttgcacat
      601 catacacctg caatagttgt acctaaagga aatccagcta atattcaaaa attggaagac
      661 ttaaataaag atggagttaa acttattttg ggagacaaag agtctaatgc tataggtaaa
      721 actacacaaa aaatacttga aaaaaataat ttaaaatcta taaatgataa tgttgtctct
      781 acagctgcaa ctgtaaatga aatgattgta cagttaacat caagtaaagc tgatgctaca
      841 atagccacaa aagattcagt ctttggcaat gaagatgtag aagttataga aattgcccaa
      901 gataaaaata tagaccaact tatatcagca ggaatcgtaa actatagtga taaaaaagat
      961 ttagctaata aatttataaa ttatatagag tcagacgaag ctaaacagat atttaaaaaa
     1021 tatggttttg aacctgttaa gtagtgatta aacttgaaaa ataaattttt tactacacta
     1081 cttactggat tttttgctat agtctttttg tttattgtat caccacttct tatgttaatc
     1141 attaaaggga taagttatgt tcctatatgc ttaaaatcag tggaagtaca gtatgcaata
     1201 acacttagta ttaaaacatc tttaatatct actatagtat gccttttatt agctattcca
     1261 gtagcatatt ttttacatat tactaaatta ccatttaaaa aactgattat acagataata
     1321 aatctcccta tgtctcttcc acacttagta tcaggaattg ctcttttact tttgtttgga
     1381 agaatgggga ttggagattc catttataag atttttaagc ttgattttat atttacaaag
     1441 caaggtatag ttttagcaca agtatttgta aatcttccac ttactattaa aatacttcac
     1501 acttcactaa atgaaagtaa tgaaaagatg atatttgttg cacgtaccct tggatgtaat
     1561 agttgggaag catttagatt tataattctt ccaaatctaa aaactggaat catatctgca
     1621 actgtaatga cttggtcaag agcacttggt gaatttggtg cagtagctat gatagcaggt
     1681 tctacacgta tgaaaactga aattattcct acatctatat atttaaatat gtctactggt
     1741 gatatagaca ttgcaatcgg aatagctgta attttgatat ttatctcact tacatgcttg
     1801 atgctttttg agatattttt taatagagag gttgataaga attaatatgt taaaaataaa
     1861 aaatttatct cttgatttac ctaattttaa attacaagaa aatagtttaa atattaataa
     1921 tcatgaatac tttgttttat taggagctag tgggtcaggt aaaactctat tcctagaaac
     1981 attagcagga agatatccat ctgctaaagg aaatgtatca tacaaagaaa atttaatatc
     2041 aaacttacct ccagaaaata gaaatatagg atttgtatat caaaattttg aattatttcc
     2101 aaatatgaat gttgaagata atataaggtt tccacttaaa ttaagaaaaa tacctataga
     2161 tgaacagatt cttaaggcta accacttgtc taaattaatg cagatagaag atataaaaaa
     2221 taggtatcct aaaaatttaa gtggaggcga aaagcaacga gttgcttttg cacgtgcctt
     2281 aatagctaaa cctgatatat tacttttaga tgaacctatg agttcattgg attatattac
     2341 aaaaaaacat ataagcaaaa ttttgaagga tgtctacata aaatacaaac caacagtgat
     2401 tcatgttact catgatattt ctgaagcaat gttttttgct cataagatag gaataatgaa
     2461 caatggaaaa ataaatcata tttttgattt aaatgaagaa ataaaaaata aaggagaatc
     2521 ttttttctat gaatatattt gatattttga gagattatct attagtacaa gtcgataaat
     2581 ataatttaaa tatggatatg ataagtattg tgtccaaatc tctatcttca aaagaagcaa
     2641 ttggaaatac taagcgtaaa gattttccaa ttatagttgg taaagaaata atgcttgaag
     2701 ctgactttaa aggagctaaa ggacaagcat ttacttctac tccatctaca tttgaaggtt
     2761 ctttaaaaga catattatca ttagatttac atgacaatcc tcatgataga agtcttttta
     2821 tagcatcatt aaatgcagtt atgaaatact taggcaagac ggatagaacc attcattgta
     2881 aaaataacga gccagaggta tgtgcaaaaa aattccctga gtttataaaa atggaatttg
     2941 gtaatccaaa agtagcaatt ataggatatc aaccagccat aattgataat attaaggact
     3001 ttttcgaaac aagagtatta gatttaaatc ctgaatttgt ggatactatt caatataatg
     3061 ttaagattga agatggtata cgagattatg aagatgtaat atcttgggct gatttagtga
     3121 tttgcacagg aagtacatta tgtaataact caattataaa ttttctatca cttaacaaac
     3181 cagtttacta ttatggaact acgatagctg gtgcatcaaa tattttaggt ttaaaaagac
     3241 tctgtttttg tagtaaataa gcaatcatat catcaaggag tacacaaata tatatgtgta
     3301 ctctttaaat ttaaaaagtt attactttta ttctaagtac taaagtaaaa ttctattaaa
     3361 atgaatcccg aataaatgta caatcccttt aaatctcaat tcctatcaat cattaatatt
     3421 ttgattagtc ttatacataa atgttttctt tattttaaac taataaaaaa attttattcc
     3481 attaatctac tctatatttt tcagcattta agaaataatt tttagtactt tgtttgataa
     3541 tataactaag acaacttttc aggagatgat taaatgacat ttaagaataa aaaatttact
     3601 ataaataaat ataaaattct atctattatt ctagttttat gtttgatttt aaccgctttt
     3661 agtagctatg tgaattatag tcataaaaaa tttaaagatt atgttgtctt ctatgttcaa
     3721 catcaagatg atgaagtgtt gtgggctggg agtgctattg ttaatgctat aaaagaaaga
     3781 ggaaaaaatc atgtatttgt ggttttagta tctactggat gtggtatatc cgtctttgat
     3841 aaatataaaa aatatgaaaa cctaacaaat atacaaaaat cagaatatag aaatagagaa
     3901 tttttagctt ctcttaatag acttggcgta ataagggaaa atataatatt attaccagaa
     3961 acaaatacaa atggtaatac tggcttcaat tacatggaaa aaattgctct tacatttgaa
     4021 gaaaaattta aaaatgtaac tcatatagca catacttata agttagatga ccatttacaa
     4081 catttaaaaa atggttctgt tatccaaaat ttatataatg ctggtaaaat aaaagatgct
     4141 aaatattttg taaaacctaa atttgctgat aaaataactt ttaataataa gatagtttat
     4201 aaagctcatt ctactgaaga ctatgaaaag gttaaaaatg cctgtcaaca atacaaaatt
     4261 gttgatgaat tagaatatag agaaggtatt ggctataagt ctgaccataa atcatttgat
     4321 aagttattaa ataataaaga tgttccttct atacttcata cacctaattt ataagaaata
     4381 tttattcttt taatttaagt tataaaaagg aaattatttg agattttgtg atatagcact
     4441 ttttgtgcta tactactttt tctcatacaa ctattaccca tatctaattt catcatctct
     4501 taaaatatat aaagatatca tcattaaaac ctataagcct tattaaatgc tttatacata
     4561 cctacattaa actacctgtt tatattaaac tacattaaaa taaatatttg ttataaattc
     4621 ttaataatta tgaaactcat ttgttattta tatttgttaa tatatgttta aacctaaaaa
     4681 attaataaac caaaataaca taagaagtgt gatagattac cctagatatt ttcataaact
     4741 tttaccttca gattaatatg ggcgtctata cttttaaatt tactatatgc attgacaata
     4801 tattaaaagt actagttttt atttcataat gaatgggctt actgttttct aatacctctt
     4861 tttggtataa aataaaaatt ggttaaaaat aaaatttcaa gggggttaat aagttgaatc
     4921 aaaatatatt ggttgtagat gatgatagag agatagttga tgctatagag ttctatttga
     4981 gacctgataa ctttaatgtt ttaaaggcat atgatggact tgaagcattg gacatactaa
     5041 tcgaaaatga tattaagtta ataataatgg atgttatgat gcccaattta gatgggctta
     5101 aaactacact taaaataaga gaaaaaagga atataccaat aattctctta tctgcaaaat
     5161 cagaagatat ggacaaaatt ataggtctta atatgggtgc tgatgactat ataactaaac
     5221 catttaatcc actagaactt actgctcgag taaaatctca acttagaagg tatataaatt
     5281 taggtgattt tagtaataca aataactcaa atgatactaa ttatattcta aaaagtggtg
     5341 gcctagagct aaatacagat acaaaaatag tttctttaga tggtgaagaa attaaaatga
     5401 caccacttga atataaaata cttacacttc ttatgtcacg aaaaggtaaa attttctctt
     5461 ctcatgagat ttatgaaaat gtctggaatg aggatgctta tagttgtgag agaactattg
     5521 ctgttcatat tagaagaatt agagaaaaga ttgaaattaa tccaaaagaa ccaaaatatt
     5581 taaaggtggt gtggggcatt ggttacaaaa ttgaaaaact taattattaa tgattctaaa
     5641 gcaaaattta ttttattatt attactgttt ctattagttt ctatattatt tgaagcagtt
     5701 tatgatttga ataataactt ttctaatatt aattattatt atagagagtt tgctctccct
     5761 tatttttatt ttttcggtac tcctgtacat ttgtcaaaag tagaattaga gtttgtctgc
     5821 ataggtatta tattatcaag ttttttattg actatactat gttttacatt gtataaaaaa
     5881 aataaacaaa ctaaagtcaa attcttagag aatgtcataa gccttttttc taaggtaaaa
     5941 atagaactta cacttatatt aactttgata ctattaacct taaggtttca tgcatttaca
     6001 agtaataaaa tattttacaa cactaattta ataatctaca attttataat tatatctata
     6061 gtctatataa t
//
LOCUS       DAEMIK010000195         6065 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEMIK010000195 DAEMIK010000000
VERSION     DAEMIK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.198"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            164..1528
                     /locus_tag="KPK87_003490"
     CDS             164..1528
                     /locus_tag="KPK87_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG2539595.1"
                     /translation="MDIKELVKMQRKYYNTGKTRNISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVSQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1667..2833
                     /locus_tag="KPK87_003491"
     CDS             1667..2833
                     /locus_tag="KPK87_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2539596.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2950..3732
                     /locus_tag="KPK87_003492"
     CDS             2950..3732
                     /locus_tag="KPK87_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG2539597.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3851..4504)
                     /locus_tag="KPK87_003493"
     CDS             complement(3851..4504)
                     /locus_tag="KPK87_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2539598.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4528..5892)
                     /locus_tag="KPK87_003494"
     CDS             complement(4528..5892)
                     /locus_tag="KPK87_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG2539599.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2289 a          760 c          915 g         2101 t
ORIGIN      
        1 ctatttcata agcttactta tacttaataa ggggcttttt ttattggaag atttatgtta
       61 tattgagatg aatttattat gatataaaaa ataatgttag gataaattta aatctttttc
      121 taaaaatgta ttagtataat tcttaaagaa aggattttta ataatggata taaaagagtt
      181 agttaaaatg caaagaaaat attataatac aggtaaaaca agaaatatat cgtttcgtat
      241 agaacagttg aagaaactta agttagttgt atcacaaaat gaagaaaaaa tacttttagc
      301 actaaagaaa gatttaaata aatcggattt tgaaggtttt atgacagaag taggcatgtt
      361 ttatagtgaa ttaaactttg ctataaagaa tataagaaaa tggagcaaaa taaaaagagt
      421 taaatcaagt atggttaatt ttccatctat atccaaagtt gtttcacaac cttatggtgt
      481 tactctcata atgtcacctt ggaattatcc atttcaatta gcacttatac cattagtttg
      541 gtcattagca gcagggaatt gtgttatatt aaaaccttct gaatattctg tgtcaacatc
      601 tagtgtagtt aaagacatag ttgaagatac tttttcaaaa gaatatgttg cagttgttca
      661 aggaagtcaa gaagaaagtg aaaagttact attggaaaga tttgattata tattttttac
      721 aggaagtact aatgttggta aaataatcat gaaatcagca tcagagcatt taactcctat
      781 tactttagaa cttggaggaa aaagtccttg tatcatttta aaagatgcta atatagacct
      841 tactgccaaa agacttactt ggggaaaact aataaatgca ggacaaacct gcgtggctcc
      901 agattatgtt ttagtgcatg aagatagaaa aaatgagtta atagagaaaa taaaatatta
      961 tactaataaa tattttggag ataatccatg taataatgag caatttccta aaatcataaa
     1021 tcagagacat tttaatcgaa tattatctct tattgataaa gataagatag tctatggtgg
     1081 aaattataat aaggagacat taaagataga gccaactata gtagacaatg ttaattggga
     1141 tgataatata atgaaggaag aaatatttgg gcctatattt cccattttaa catataaaga
     1201 tttggatgaa gtaatacaaa aaattataca aatgccaaat ccattggcac tttatatttt
     1261 tacaaaaaat aagtacctag aaaataaact tttagaaatg attccagcag gaggatgttg
     1321 tatcaatgat acagttactc atatagctac gaattactta ccttttggtg gtattggtga
     1381 aagtggtatg ggctcatatc atggaaaagc tggattcgat acatttactc actataagag
     1441 tgtgttaaaa aaattaaatt tggatgtccc tattagatat gctccatacg ataataaatt
     1501 aattaaggtt cttaaaaaga taatgtaggt tatattgtag aataataaat ataaataaaa
     1561 ttgagtattt gtattctgaa tatattttat ttttaatttg gtatatttgt tataaaagtg
     1621 atgattttgt gataaaattt aattatatag aaataaggag gaaaaaatga aaattaattg
     1681 gaatacaaaa tacacaacaa tagcaattta tacatttata atagctgcta gtagtattat
     1741 attttatcta gtatcatcac aaattgatgt attttctaat aatttagatg ctatatttac
     1801 aacattacaa ccttttataa taggatttgc tattgcatat cttcttaatt ttattttaaa
     1861 gttttatgaa gacagaattt ttataaaaag tgagaaatta aaaaaactaa aacaaagttc
     1921 aaaaagaggc ttgggtcttt tattaacata tgcaacagct acattgatac tttacttatt
     1981 tatgcatttc gtattgccac aagtaattga gagtattgtt ggactagcaa atgatatacc
     2041 tatgtatgta aacaatgcaa ctaagcttat tgataaatta atgactgatt taaatcttga
     2101 tgagcaatac tttaatttag cagttgataa gtggaatgag tttgttacat atattataaa
     2161 gtttgtaaca gatttaatcc caatattagg aagtatgtta aagaatgtag cctctagtat
     2221 atggaatatt gtactaggat taattgtttc agtatactta ttaattgata aagaaaaatt
     2281 ctatggatta agcaaaaaaa taacttatgc agtatttaca gaaaaacaag ctgctagaat
     2341 tttagaatta actcatagaa gtaactatac atttggaaga tttttaggtg gcaaaatatt
     2401 agattcattc attataggta ttctaacatt tgtaatactt acattagtta aaatgccata
     2461 tacattatta atatcagtta taattggtat tacaaatatt atccctttct ttggaccttt
     2521 atttggagct ataccatcca cgctaatagt gatgtttgta tctcctataa aagctttctg
     2581 gttattgtta attatattaa taatacaaca gatagatgga aatattatag gacctaaaat
     2641 acttggagat tcaattggta tatctgcatt ttggattttg ttctccttat tggttgcagg
     2701 aaaattatta ggttttattg gaatggttat aggagttcca atgtttgcag taatttattc
     2761 tataattaag gatattgttg aaagtaaatt ggacaaaaag ggattaccga cagatacttc
     2821 cgattatatg tagctaaaaa atattatgta taattaaaaa tatataaatt aagcaatatt
     2881 taaagcactg agaaagttaa actcttggtg ctttttagat ataaaattat tatagaagag
     2941 gtgttttttg tggcatttat agaatgtaaa tttaaatcta aatatactgg aggagaaact
     3001 gatataattg taattttgcc tgtagcaaat ggaaaagaac tttttagtgg taaagagata
     3061 tatgattttg aaggtaaatt taaaacatta tacttatttc atggtttaga agatgaccaa
     3121 agctcatgga ttagatatac taatgtagag cattatgcta aaaataaaaa tatagcccta
     3181 gtaataccaa gagtagaaac tagtttttat accgatatgg cgtatggaca ccaatatttt
     3241 acttttgtaa gcgaggaatt acctaaattt gttagagcta tatttccttt atcagataaa
     3301 agggaagata actttgtagc tggtatgtct atggggggat atggagcttt aaaattagcg
     3361 ctttcaaaac caaaagagtt ttcggcagtt gcaagttttt ctggttcgcc tgatatgatt
     3421 catgagttag aaaatcaaca aatagaaacc aatgcagaca ttctatttca cgattttggt
     3481 agaccagaag acgctagaaa tagtgagaat gatttacttt acatacttaa gaaccttaaa
     3541 gaaagaaatg aagatattcc taaaatttat caattttgtg gtgatagaga ttttttatat
     3601 aaaaataatc agatgtttaa aaaatatgca gattctctgg gtgtagatgt tgaatatgaa
     3661 gagtgtgtag gggggcatga atggcgtctt tggaatttat ggataaagaa atttatagat
     3721 ataattggat agtacactta tggtattaac aggtatacta taaaatatct ttaaatatga
     3781 taaagactga aatcctaaaa tcaaattatg attttaaaat ttcagtctct aagaatttaa
     3841 taaaatttta ttaagttctt acacgagtta ttattggagt aaaattgtca actatatcta
     3901 tatagtattt actcaattcc tctaaaatct cttcgcataa atccgtacta tgaccaagat
     3961 taataagaaa ctgttttgta aaatgcttta ttacagcttg agaagatata tcgtacttgt
     4021 gtgaagtata aactctaatt atttcaggtt tcatagcaag tataccaata caagaaagta
     4081 ttaattcatc atgttctata gattctttat cattaacata tcttttcatg accatttcgg
     4141 ttacacttct ttgatagctt aaaatagctt cttcataagc tatatccaaa taaaaatttc
     4201 tatatgcttc gttttttaga aggaaacaaa ggtcagcatt catagcaaga aagaacatca
     4261 tcatagtacc ttcttcatgg tagttataga ttaaagactg catttcattt cttatatctt
     4321 catatattat agctcctatt tcgcttttac ttgagaagta atagcttatt agacctaaat
     4381 ttatattagc tctttctgca atttgcctag ttgttgtctt atgataacct tgctcaaaaa
     4441 ataattctct agcagcactt ataatactag cttttgtttc tttgcccttt tctgattttg
     4501 acatatttac tgtgtatacc tctcttttca atcttttttt gaaaatttgc taactataaa
     4561 tattgtaata gaaccaccta ttataggaac caaaattctg tcaaatacag taagaatacc
     4621 aatcatattt aataatgcca atataacact tactataaca gcatagatag ctcctttttt
     4681 tgtagcacct ttccagcaaa gaccaattac aaatggtgca aatattacac ctgtacataa
     4741 tggagccggc caaaacaata attctaataa actatcaaat ttaagtgcta tgattgcacc
     4801 tattatagct gtcaaagctg atgcaatttg agaatacttt agagtatttt ttaaatcatt
     4861 aaattctata ttaggatgta ataatttgtt ataaatatct cttgatataa tagcactcat
     4921 tgatgtcatt aatgaatcgg cagtagacat tatggcagcc aatatagcag ctaagaataa
     4981 tacagatatt aaaggtggca aagtagtcga tatcatttga ggtaaaacct gaacagatga
     5041 tatgtttgga aataaactac caccaatagc tccaattatt cctataggga aagcaagaaa
     5101 taatagtata aatcctgcaa ttacactact tttaactgca tttttagcat tatcagcaca
     5161 ttgatatctt aaaaaacttt cataagaaaa tattgagcct aaaactaatg gtaaaactgt
     5221 atacataaaa tcactaatcg atttatttcc tggaaagaat ggttctttta ccattgattg
     5281 taacataagt aaagggttgt cgccaactaa ttttaagctt atataagcta ctattggcaa
     5341 aaacactata caaatagcaa gttgaataaa tgctgatact gcaactcccc acattcctga
     5401 tgtataagta tatataatga taactataac tcctataatt acagctatat cataaggaat
     5461 accaaatgca gtagatacag aaccaaaaga aatcaattga ctacccataa tagctagtat
     5521 agatattggt gttacaagac tttgataagc tcttaaaaat acactatgac catatctagt
     5581 ttcaatataa tcagcaggtg ttatatcaca agataagctt cttaattttc ctgcgaaaaa
     5641 tgcatttata aagcaagcta aagctgcacc tatagtagaa tacattccaa cccaaagtcc
     5701 tctttcagct ccccattgaa ctccaccaac taagcaccct cctccaaaag atgtagcagc
     5761 aatggttgca gctagtatag gcaaaggcag atttctacct gcgattagga aatcggatgc
     5821 tgttttaact ttttttgaaa aataaaaacc aatccatatg tatattgcac acgtaacaat
     5881 taaaagtaac atagtctttt ctcctttata aaatatttta ataaaatata caaaatatat
     5941 ttaaaatata ttttatataa aagtagtacg atatttgttt tattggtttt agattaatat
     6001 agctataata atttatgata ttagatggaa attacgtaaa aatagaaaat tgaaaattct
     6061 gaaat
//
LOCUS       DAEMIK010000196         6046 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEMIK010000196 DAEMIK010000000
VERSION     DAEMIK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.199"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..716)
                     /locus_tag="KPK87_003495"
     CDS             complement(<1..716)
                     /locus_tag="KPK87_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2539600.1"
                     /translation="MNAINWKKLLLPNIPYLLFVYLFDKVGQAVHLAPGADLSGKVLS
                     LGEGFSAAFANPLPSLAPMDLLIGILGAVLIRLIVYVKGKNAKKYRKGVEYGSARWGT
                     AEDIKPYTDPVFQNNVLLTQTERLSMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKP
                     NLMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMKYNPFAYLRSEKD
                     ILKLVNTLIANTKGDGEKAG"
     gene            complement(713..1195)
                     /locus_tag="KPK87_003496"
     CDS             complement(713..1195)
                     /locus_tag="KPK87_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG2539601.1"
                     /translation="MQEETNEKTIALYIKTGKLTAQQLQKAMKALLAQMKKQHDKQKI
                     PHGKQTLKQLMKQNAGVSNIEITKDNIKAFESTAKKYGIDFALKKDSTETPPRYLVFF
                     KGRDADALTAAFKEFSAKKLTQEQNPSIRKLIVSLKEKAAALNAQRDKVKNKDRGIAR
                     "
     gene            complement(1290..1508)
                     /locus_tag="KPK87_003497"
     CDS             complement(1290..1508)
                     /locus_tag="KPK87_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBG2539602.1"
                     /translation="MTQKTGALIFDETADRYDIRFDVNDYYGGLHCGDCMEVFVRGKW
                     KPTRMEYGDNWYLVGIRAADLSGLRVRI"
     gene            complement(1533..2507)
                     /locus_tag="KPK87_003498"
     CDS             complement(1533..2507)
                     /locus_tag="KPK87_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539603.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPAL
                     DCPILENPTADKPILENPTPDNPTSENPTQLNKELLKTNLPNKEKLNTDISSTHSIPF
                     HSLNPSPLEDAAQPPERKRKETTDAYSVYEEIIKDNIEYDYLIQDRYLDRDRIEEILA
                     LILETVCTKRRTIRIAGDDHPAELVKAKFMKLNSEHIRFVLDCMQENTTKIRNIKQYM
                     KAALFNAPSTIGSYYTSLVSHDMADGTFYGKPKKSGIPDYTCDEGESL"
     gene            3006..3371
                     /locus_tag="KPK87_003499"
     CDS             3006..3371
                     /locus_tag="KPK87_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539604.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3394..5781
                     /gene="cadA"
                     /locus_tag="KPK87_003500"
     CDS             3394..5781
                     /gene="cadA"
                     /locus_tag="KPK87_003500"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG2539605.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT         1855 a         1006 c         1305 g         1880 t
ORIGIN      
        1 tccccggctt tttccccgtc ccctttggtg ttggcaatca aagtgtttac cagtttcaaa
       61 atgtctttct cgctgcggag ataggcaaag ggattgtatt tcatgctttt tttgaagtta
      121 atcgtgttca gcacttttat ccggtagccg ccccgctgta agagcttccc gcactcgact
      181 aaaaccgtac ctttcgggtc ggttacaacg tagcttgagt gcatttgcat taaattcggc
      241 ttcacaaaaa atctcgtctt gccgctgcca cttcccccga taacaaggat atttttattc
      301 cttgcatact tcggctgctt cgggcggctg ttcatgctaa gccgttccgt ctgtgtcagc
      361 agcacgttat tttgaaatac cgggtcggtg tagggcttaa tatcttcggc agtcccccaa
      421 cgggcagagc cgtattccac acctttgcga tatttctttg cgttcttgcc tttgacatag
      481 acaatcagcc ggataagtac cgcgcctaaa attcctatca gtaaatccat aggggcaagg
      541 ctcggaagcg ggtttgcaaa ggcagcggaa aagccctccc caagcgacag caccttgccg
      601 gacaggtcag cccccggcgc aaggtgtacc gcctgcccga ctttatcaaa gagatacaca
      661 aagagcagat agggaatgtt gggaagcagc agctttttcc agttgatagc gttcatcttg
      721 caatccccct gtctttgttt tttactttgt cccgctgtgc gttcagagcc gccgcctttt
      781 ctttgaggga aacaatcagt ttgcggatag agggattttg ttcctgcgtc agcttctttg
      841 cggaaaactc cttgaaagct gcggtcagtg cgtccgcgtc ccgtcccttg aaaaacacca
      901 aatagcgggg cggggtttcg gtgctgtctt tctttaaggc aaagtcaatc ccgtattttt
      961 tcgccgtact ctcaaaggct ttgatattgt cctttgtgat ttcaatgttg gaaacgcccg
     1021 cgttctgctt cataagctgc tttagagtct gctttccatg cgggattttc tgtttgtcat
     1081 gctgcttttt catctgtgca agcagggctt tcatagcctt ttggagctgc tgcgccgtca
     1141 gctttccggt cttgatgtaa agggctatgg tcttttcgtt ggtttcctcc tgcaatgggt
     1201 ttcctccttt cgtgttctat ggtggtatgc ccgccgccgg gaagtggggc gggcatgaaa
     1261 aaaagagcgt ccgttttcac gacgccctgt taaatccgta cccgcagccc ggaaaggtct
     1321 gccgcccgaa tacccacaag ataccagttg tccccatact ccatacgggt aggcttccat
     1381 ttgccccgca caaagacctc catgcagtcg ccgcaatgca agcccccgta atagtcgttt
     1441 acgtcaaagc gaatgtcgta gcggtcagcg gtttcatcaa aaatcaaagc tcctgttttc
     1501 tgtgtcatta taaaatcctc ctttgggttg gtttacaggc tttcgccctc gtcgcaggtg
     1561 taatcgggta tgccggattt tttcggtttc ccataaaatg tgccgtctgc catatcgtga
     1621 gatacaaggg acgtgtaata gctgccaatc gtagacgggg cattgaaaag ggcagctttc
     1681 atgtactgct tgatgttgcg gattttggtg gtgttttcct gcatacagtc cagtacaaag
     1741 cggatatgtt cgctgttcag tttcataaat tttgctttta caagctctgc cgggtggtcg
     1801 tccccggcga tacggattgt tcttcgtttg gtgcagacgg tttcaagaat aagggcaagg
     1861 atttcctcta tcctgtccct gtcaaggtat ctgtcctgta tcagatagtc gtactcgata
     1921 ttgtccttga tgatttcctc atacacgcta tatgcgtctg tcgtttcctt tcgcttccgt
     1981 tccggcggct gtgccgcgtc ctctaaggga gagggattta gggaatggaa aggaatggaa
     2041 tgggtacttg atatatctgt atttaatttt tctttgtttg gtaagttagt ctttaatagt
     2101 tctttattta attgcgttgg attttccgac gtcgggttat ccggtgtcgg attttccaat
     2161 atcggcttat ccgctgttgg attttccaat atcggacaat ccaatgccgg gggctgtggc
     2221 tgttcgtaaa tggtgtactc gatagcggtc attttgcctt tctcgtcgcg tccctgccgc
     2281 cttgtgatat atccggcttt ttcaagctcc catacagcgg tgcggatagc gtcgatactt
     2341 tcccggttga taagggacag ccctgcaagg gtgtagtccc aatcttcggg aagtgagagc
     2401 atttgcgata acaagccctt tgcctttagg gaaagttcct tgttgcgtaa atggtggttg
     2461 ctcataaccg tataaccttt gttcttttcc acgcgaaaaa ctgccatagc ttcgtcactc
     2521 cttttcgttg gatttgttac gcagtagaaa cgcggtttgg agggaaaagg tatcaaccct
     2581 tgtctttctt atctccgcgc tctaagagcc acataaatca agacttcttt tgcccttact
     2641 gcgtaacatg agggcataaa aaaagcagcg ttcctgttct cctgtgtagg agtaagaaac
     2701 gccgccttat aggtggtgct tattttattt tttcttatcg acgtgccgcg caaggtggtg
     2761 tagcggtcta tccggcagct tcgcaggtgc ttttgcatat caaggcgcaa tccgttaaaa
     2821 gtagtaaatg agtagtaaaa tcaatctgca aacctgcgaa tatgcccgta aatcaagcat
     2881 ttttcaagac aatcaactaa tttaaagagt aaattaataa aaattattgt tgcaaattta
     2941 gaaatgataa catataatga acatatgaac agttattcat atattaaaat aatggaggat
     3001 taaaaatgag agaagagata aatgattgta attgtaatat agtacatgaa gaaatagtaa
     3061 cagaggctaa atcaactatg ccagatgaag aaatgttata tgatttagca gaattattta
     3121 aagtatttgg ggatactact agagtaaaga tattatatgc attatttgca aatgaaatgt
     3181 gtgtatgtga tatagcgagt ttattaaata tgactcattc tgcaatatct catcaactta
     3241 gagtattaaa gcaggctaga ttagtaaagt ttagaagaga aggaaaaaca gtgtattact
     3301 cattagatga tagtcatata agtcagatat ttgattgtgg attaaatcat attagagaga
     3361 cttataaatg ataaatgaga ggagtgaata tatatggaag caaataattc aattaaaaaa
     3421 gaatttatac taggaggatt aaattgtgct cactgtgcag aggagattaa taataaagtt
     3481 tctaaactac aagaagttaa atcttccaat ttaaatttta taaataaaaa acttacagta
     3541 aatataaagg aaagttttaa tgaggatact actatagaaa agataataga tataatagat
     3601 tcaacagagc caggtcttga tatacaaata agttctaaag aaaatgcagc tagtaaaaca
     3661 tcaattaaaa aagagcttat attaggagga ttaaactgtg ctcattgtgc agaagagatt
     3721 aataacaaag tttctaaatt aaaagaagtt gaatcttcta atttaaactt tgtaaataaa
     3781 aaacttacag taaatataag taataacttt gaagaggatg atgttataaa taaaataaaa
     3841 gaaataataa attcaacaga gccagggctt gatatacaag tagggtctac tgacaaagta
     3901 aaagggagaa ctactgaaaa atcaggagct gtaaatgata caaataagaa agaattaatt
     3961 ccacttataa ttggagcact tgtatatata tttggtatat atcaaacagc tacaggttat
     4021 gaaagtcagt ttagcaatat agtatttata gttgcctatg taatagtagg tggtgatgtg
     4081 cttttaagag caataagaaa tatatcaaaa ggaagagtat ttgatgagaa ctttttgatg
     4141 gctctagcaa cagtaggagc attagcaata ggggagttat cagaagcagt aggggttatg
     4201 cttttctata aagtaggaga gtatttacaa ggtgttgcag ttggtaaatc cagaaagtct
     4261 ataacttcac ttatgcaaat aagaccagac tatgcaaatc taaaagttaa ttctgaagtt
     4321 aaggtggtgt caccagaaga ggtaaatgta ggtgatataa tagtagtaaa acctggtgaa
     4381 aaagtaccat tagatggtgt agtagtagat ggagtttcta tgttagatac ttctgcactc
     4441 acaggagagt cagtgctgag agaagttgaa aaaggagatg aaatcttatc aggtgttata
     4501 aataaaaatg ctttattgag tattgaagtt acaaagagtt ttggagaatc aacagtttct
     4561 aagattcttg accttgttga aaattctagc attaaaaaat caaagacaga aaactttata
     4621 tctaagtttt caagatacta tactccaata gtagtaatag ctgctttatt aatagcattt
     4681 gtgccaccac ttgtaatctc aggtgaggta tttagtgatt ggttatatag aggattgata
     4741 ttcttagtcg tttcttgccc ttgtgcatta gttttatcaa taccacttag tttctttagt
     4801 ggaattgggt ttgcatctaa aaatggtatc cttattaaag gaagtaacta tttagaagct
     4861 ttaagaagtg tagatacagt agtatttgat aaaacaggaa cacttactaa aggtgtgttc
     4921 aatgtaacta agttaaatcc tgaaggtata tcagaagaag aattattaga atatgcagct
     4981 attgcggagg taaattcaaa tcatccaata gcaaagtcca tattaagtta ctataataaa
     5041 aaaattgatt tagatacaat agacagttat gaggaaatag cagcttatgg aattagagta
     5101 aagcataatg gcaattttat attagcaggt aatgagaaac ttatgaaaaa agaaaatata
     5161 tcttattcat cagctaaaga agtaggcaca gtagtctata ttgctgttga taaagtatat
     5221 agaggttata tagttatctc tgatgaagta aaagaagata gtaaaaatgc cataagaagc
     5281 ttaaaagaaa taggggtaaa agaagttgta atgttaactg gagataatga gaaagtagca
     5341 aaaaatattg ctcaagaatt agaattggat acagtttact caaatttact tcctaatgaa
     5401 aaggtagata gattagaaga tttatatgaa ggaagaactg aaaaagagaa aatagcgttt
     5461 gtaggtgatg gaataaatga tgctccagta ttagctcgtg ctgatgttgg tatagctatg
     5521 ggaggcttag gttcagatgc tgctattgaa gctgcagatg tagtgcttat gacagatgag
     5581 cctagcaaga tatctaaagc aattgaaata gcaaacaaga ctaataagat agtgtggcaa
     5641 aacataatat ttgctctagg agtaaaaatt atagttatga tactaggtgc tggaggagta
     5701 gctactatgt gggaagctat atttgctgat gtaggtgtgg cacttatagc agtagtaaat
     5761 gctatgagag ctatgagata agggataaat gtaaatccat actatttaaa aaacttataa
     5821 ttatatattg aagtatgtaa taatgaaagg tatcaataag tttcttttaa ctttattggt
     5881 acctttttat tggagcgctt tgcatatata aatttatatt cattatccat tatgtttatt
     5941 taatgtattt atttagtgta gagcttttat attgtaattt taaaatttag acatagaagg
     6001 tttattaata agtttatatt aagtttaaat tagattcata aaaatt
//
LOCUS       DAEMIK010000197         5850 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEMIK010000197 DAEMIK010000000
VERSION     DAEMIK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.200"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            7..1359
                     /locus_tag="KPK87_003501"
     CDS             7..1359
                     /locus_tag="KPK87_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2539606.1"
                     /translation="MTVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTG
                     YSNWRKFELDVTNLLKKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLN
                     ELTDINETFARVSADNFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLS
                     VSVYSDRASLAKSSIKNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQ
                     PKYNIKLDKFCGSEALVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIR
                     SLFDKGISPLPISVNFSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTD
                     TLFNVSRNLQDIGFIVAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIV
                     IKSIVSLIKQLGIRVVAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI
                     "
     gene            1392..4829
                     /locus_tag="KPK87_003502"
     CDS             1392..4829
                     /locus_tag="KPK87_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG2539607.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNGLENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            4847..5593
                     /locus_tag="KPK87_003503"
     CDS             4847..5593
                     /locus_tag="KPK87_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG2539608.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5542..5850)
                     /locus_tag="KPK87_003504"
     CDS             complement(5542..5850)
                     /locus_tag="KPK87_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2539609.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2434 a          581 c          904 g         1931 t
ORIGIN      
        1 gacataataa cagttacaat tgctggatgg attatacttt catttttatt tttaggtatt
       61 ataatttaca tattatattc aaaagcaaat agtcaaaaaa ctatagaaaa agtagcatat
      121 acggattttg taacaggtta ttccaactgg agaaagtttg aattagatgt tacaaattta
      181 ttaaagaaaa catcacaaaa taataaatat gcaatggtca tattcgatat tgataaattt
      241 aaagcaatta atgatatata tggccataaa aagggaaatt taatattaaa agatattgca
      301 gatactttaa atgaattgac tgatatcaat gaaacttttg ctcgtgtaag tgctgataat
      361 ttcaatatat tattaacata taacaaaaaa gaggatataa taaatattat caaaaagatt
      421 atggcaaata atgaacttgt aaacttatct tttggaattt atgaaatcaa agataaggat
      481 ttatctgtaa gtgtatatag tgatagagct tcacttgcaa aatcatcaat aaaaaataat
      541 agtgatgtaa attttgcatt ttttaatgac aaattaagag agaaattatt atttgaagat
      601 aagatagaaa aagaaatgga atatgctctt gaaagtgggc aattcgtaat gtatttacaa
      661 ccaaaataca atattaaatt agataaattt tgtggaagtg aagccctagt cagatggcaa
      721 tatacagaaa aagaggtcat atatccaggt gattttattc ctatatttga aaaaaatgga
      781 tttattagaa agattgatat gtacatatta gaacaagctt gtaaagaaat ccggtcttta
      841 tttgataaag gaatttcacc tttacctata tctgtaaact tctcaagagt tgattttttt
      901 aaaaaagatt ttattgaaaa catagtaaat atatgtgata gatataaaat accatatagt
      961 ttaatagaaa ttgaaattac tgaatctagt atgtttggag atactgatac tctatttaat
     1021 gttagtagaa atctacagga tattggcttt atagttgcaa tggatgattt tggctcggga
     1081 tattcttctg taaatatgct taaaaatata ccattaaatg taataaaatt agatagagga
     1141 ttttttgttg atgataaaga tgttgataag agtcaaattg ttataaaaag catagtttct
     1201 ctcattaagc aattaggaat aagggttgtt gctgaaggaa ttgaaacaag aagtcaaata
     1261 gaaatgttaa aaaaagcaaa ttgtgatatt gttcaaggat attatttttc aaaaccatta
     1321 ccaattaaag aatttgaaaa attagtctat aaaatatagt cagcgagtct taaatgggag
     1381 gtggtgatta agtgataaga gcaaaatcgt taaaaaatgt actgagtatt gttttaatat
     1441 ttttatttac aataatttct ttttctgatt ttgcatttaa aactaatgtc caggaagaag
     1501 aaaatagtaa aattaattta agggaaatat ccctacaaag ttccaatgct ataaaaataa
     1561 aattagatag ttctctatcc tttattaaaa gtgtggcaaa tatggtttct tattttaata
     1621 ctatagataa ctcaaaaata gttccattat tagctaatat atcaaaagaa tctgactttc
     1681 aaagaatctc tattgtatct cctgatggaa taagtcatac cagtgatggc aaaattatca
     1741 atgtgtcaaa gtatcagtat tttaagaaag ctattaaagg agaaagtaat atatctgaag
     1801 ttgtgaaatc tccaattgat ggaaaagata cttttgttat agctactcct gtatttaata
     1861 agaataacaa aataattggt gttttatacg gttcttacta caatgataaa ttagctgaat
     1921 ttatagatat aacaagtttt aatagtgaag ggtacataga tatatttgaa tctagtggtc
     1981 aatttgtttt aaaatcttca catacaaaca aactgattaa gtcaagtagt aacatatatg
     2041 atatatttaa aaaagcttct tttgatgatg gttatagcta taaaaaaata tatgaagata
     2101 caaaaaataa caagagtggc tttacctcat atgaatacaa aggagaaaat cgttatgcta
     2161 cttatacacc cattggtgta aatgactggt atgtaatttc tgtgatacca caatatactg
     2221 taagaagaaa cattgataaa ataaatcaat tagcattttc tcttattttc aaggtattct
     2281 tattgtttat tatgcttata atatatatta tttataaaaa caataaatct cagaaagata
     2341 tactaaaagc taacaaagaa atctcatcta ttacaaatag cattcctgga ggagtacaaa
     2401 agtgtaccct gggtgaaaca tgcaatatta attttttaag tgatggattt atagagttgt
     2461 ttggttatac aagagaagaa ataaaaacat tgtttaataa tgatttttat agtttaatac
     2521 ataaagatga tgttgaaaga ataaaaaaag aaatttttaa acaactttac aaaaataaaa
     2581 ctgttgaatt agaatatagg attataacaa aaaatggtaa tacagtatgg gttttagata
     2641 aaggggagct tataacagaa cctgatggaa aagaatattt ttgttgtgta actgtagata
     2701 ttactagaac aaaacaatta gaacaagaga tggaacaaaa aaatctagaa cttgaaatga
     2761 tatcagaaaa tgttttaggt ggtataatta taacagaatt taatgatgat tttactataa
     2821 aatacgctaa ctctggttat ctttcaatga ttggatatac acgtgaacaa ttagaaaatg
     2881 aacttaaata caaagcttgt aatcttgttt tttatgaaga tagaaagaag gctcttgaaa
     2941 gtatacaaca tcaattaaac acaaaaggaa tagctgtata tcaacataga ataagaagaa
     3001 gagatggtaa tattatttgg gtttctataa aaggaaggct tattgttgat gatgatggta
     3061 ttgaaaaagg tatttggatt ctaaatgata taacagattt aaaaaatgca gaacaaaaac
     3121 ttaaaataaa tgaagaaaga tttagaattg cattaaagca aacatcaaac actatatttg
     3181 agtatgatat aaaaacaaag tcacttttaa atactagtaa agagcttgat aaatataata
     3241 tttataaaat tactaaaaat gttccatatt ctctagtaga attaggagtt attcataaag
     3301 attttagtga tgattttata aatatgtatg aaaatgtaat aaatggagca aaaaaagcaa
     3361 gttgtgtagt taagatgaaa cttccagatg aaaattatat atggaataaa gttaccttga
     3421 ctacgatttt tgatgaagat ggtaatcctg taagagcaat tggtatacag gaagatataa
     3481 gcaaacaaaa ggaagcagag ataagataca atcaagaagg taagtatcat tcagctatat
     3541 tgtcggaagc aatgataact tatgaaataa atgtatcaaa ggatactttt atcaaaggaa
     3601 atgaaagttg gcaaaaacca tttggaatta agcctgacaa taactattct caaatggtaa
     3661 aaactatagc aaatagtatt gtacataaag acaataaaga aacattttta aatacatttt
     3721 tatgtgaaaa cttattggat gcatataaaa ataatgtgac tgaaataaaa ttagaatatc
     3781 gaagaatgaa tcaagaaaat aaaatgattt gggtcatttg tacaatgcac ttaataaaag
     3841 attctattac gaacgatata aaagcacttg cttatgttaa agatattgac aagcaaaaaa
     3901 atgaagaaat tagattaaaa ttaaaagctg aaagagatac tcttacagga ttatataata
     3961 aaggtactac agaaaaattg attaatgatt tcttgaactc tgaggaagca agcttaggaa
     4021 tgcatgcatt tttaattgtt gatatagata attttaaatc tgtgaacgat aatttaggac
     4081 atgtatttgg agataaagta ttatttgaag tttctaaaaa cctaaaacct atatttagaa
     4141 aagatgatat agtaggtaga attggtggag atgaatttat agtattctta aaaaatattc
     4201 agtcttttga gaataccaaa aagaaagcag aaaaaatatg cgatatattt agaaattctt
     4261 atacggaaaa taaaaagaca tataaaatat ctggaacagt tggaatttca tttgtaccag
     4321 aacatggaac tgtatttgaa gaactttata aaaaagcaga catagcactt tattatgcaa
     4381 agagcaaagg aaaagataag tatgcacttt ataatgattg tatctaccaa aatggacttg
     4441 aaaataatat aagtgataat aatataaaat tagcagataa atgtgagaaa tccaaaccag
     4501 tacttaaaga aattcttgat tctattgata attatatcta tatagtgaat ccaaatactt
     4561 atgaacttct ttttgtcaat aataaggtgt ctcgtttgga tttagagata aaattaggag
     4621 aaacctgtta taaatcacta ttaaaatctg atagaccatg taataattgc cctcttaacg
     4681 gattagaaga aaataagcat tcaaaattca aaatagagac aaatacattt ggtaaaaata
     4741 tcagtatagc aagttggatt aattgcatag atggagaata caactgtctt atagagtgta
     4801 tagattcttc tttatacaaa aataaataaa aaaggactat gaagatatgt taaatagtat
     4861 aactttgata cttggtataa tatttacaat ttactatttt tatctcaaaa tagtttttgg
     4921 aggaatttca tttagtgaga ttttcttagt tgtaggaatt gtactaatta tctatcaaat
     4981 atttaagaaa aaaattaaag aaaagaaggt tttttatagg gtgttaaaaa taataatcag
     5041 cattttttta atagtatttg tagttactga gagtctaatt attttttatc ctaaaaatag
     5101 tttagaatca aaaagtgatt atctcttaat tttaggagct tctgtaaaaa agactacacc
     5161 aagtacaacc ctgaagggta ggttagacac tgcattgaaa tacctaaaaa ttaatgataa
     5221 ttgttatgtg gtagtatctg gtggtaaagg aagtggagaa aaaataactg aggcaaaagc
     5281 tatgaaagat tatcttataa aaaatggtat agataaaaat aggataatag aagaggataa
     5341 atccacgaat acttatgaga attttaaata ttcaaaattg aagatagaag aacatagtca
     5401 aagaaaacta agcgatttaa aagtaaaaat agtaacaaca gattttcatg ttctcagaag
     5461 taaaatatta gcatatagaa atggatataa aaatacgtct ttttatgcta gtaaatcaaa
     5521 actttcattt gtaccaactt attatacaag agaatttttt gcattatgga aaacaatcat
     5581 ttttgataga taataaataa aaagactaga tataaactca tatctagtct tttctattcc
     5641 cttaattcta atccattttc caactttact tgttgtagaa atttcaacat tgggatttac
     5701 aagtaattgc ttataagaag ttttatattt ttcgattcca aaatataatt ttccttcata
     5761 atccattata aatccaaatg gtcttactct tggtttatta tcttcaactg tagcaaaata
     5821 gaatgttgag ttttctttta aaaaattcat
//
LOCUS       DAEMIK010000198         5841 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEMIK010000198 DAEMIK010000000
VERSION     DAEMIK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.201"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(317..2149)
                     /locus_tag="KPK87_003505"
     CDS             complement(317..2149)
                     /locus_tag="KPK87_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539610.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2142..4388)
                     /locus_tag="KPK87_003506"
     CDS             complement(2142..4388)
                     /locus_tag="KPK87_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539611.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRFSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4456..4917)
                     /locus_tag="KPK87_003507"
     CDS             complement(4456..4917)
                     /locus_tag="KPK87_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2539612.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5222..5788)
                     /locus_tag="KPK87_003508"
     CDS             complement(5222..5788)
                     /locus_tag="KPK87_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG2539613.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1873 a         1086 c          629 g         2253 t
ORIGIN      
        1 cttcaatata atcatcaact attaaaatta aattctattt ataataaaat ttaaagaata
       61 aaattaaata tacattttga taatcacctc caacattttc cttactacat attataataa
      121 aataaacact ttgtaaagac ataaaattat gtggctatct caaaatatag agaatcctct
      181 ttttcattgt taaaatgata aaaatacact ttatttcata cacaaaagag tgccttatga
      241 actaaaaagt tcattttaag gcaccctcat ttattttatc ttattaatta catgtctata
      301 ctaagatagt tttttgttac attgcttcat cttcttcaaa ttgagaattg taaagattgg
      361 catagaatcc gcctttcttt agaagttcct catgatttcc ttgttcaact atatctcctt
      421 cattcattac aagaatcata tcagcatcac gtatagttga taatctgtgg gctataacaa
      481 aacttgttct accttccata agattatcca tagctttttg aatcaatact tctgttctag
      541 tatcgacaga acttgtagct tcatctaata ttaaaatttt aggatcagct aaaatagcac
      601 gagctatagt caataattgt ttttgacctt gagaaacatt acttgcttct tcatttaatt
      661 tcatcttata tccatctggt aatgtcttaa taaatctatg aacatgtgca gatttagcag
      721 cctctataac ttcttcatct gtagcattca acttaccata tcttatattt tccataattg
      781 aaccactaaa taaccatgta tcttgaagaa ccataccaaa catttctcta agctcactac
      841 gattaaaatc tttaatatta tgaccatcaa tcagtattga accgctatta acatcataaa
      901 atctcattaa cagtttaact attgtagttt tacctgctcc tgtaggtcca acaatagcaa
      961 ctttttgacc aggtttaaca tttactgaga aatcatttat tatagtcttg tttgggttat
     1021 atccaaagtt gacattttca aaatcaattt taccttctaa accatctatt gaaactgcat
     1081 tttcaacaat ttggacttct tcttcctctt ctaaaaactc aaatactctt tcagaagctg
     1141 ctgctgttga ttgcaataaa ttggcaacct gtgcaatctg agtcattggc tgtgtaaagt
     1201 ttcttacata ctgtataaat gactgtatgt caccaacctc aattgttttc ttaattgcaa
     1261 gccaacctcc taagattgat accattacat atcccaagtt tccaataaac atcataagtg
     1321 gttgcattat acctgataaa aattgagatt tccacgctga attatacagg ctatcattta
     1381 gtttatcaaa ctcttcaatt acttcatcct cacgattaaa tgcttttact attgtttggc
     1441 cactgtaagt ttcctcaact tgaccattta catttcctaa atattcttgt tgatatctaa
     1501 agtatttttg tgatttcttt attattcttg aaattacaaa cattgatatt ggtaatatta
     1561 aaactgccac taatgtcata attccactta tagataacat cattatcaaa acacctatca
     1621 tagttgttac tgatgttata agctgtgtca tactttgatt taaactttga tttaaagtat
     1681 ctatatcatt cgtaattctc gataatattt caccatgcgt cttagtatca aaatacttca
     1741 ttggtaatct atccaatttt gctgaaatct cttttcttaa attataggat actttttgag
     1801 aaattccact cattataaat ccttgtataa aagagaacag tgcacttatc aaataaagga
     1861 aaagtaaaaa ggttaagatt tttccaattg cattaaaatc tatacctacg tttcctccag
     1921 aaactttact cactagccct tcaaatatct ttgtagttgc ctttcctaaa atttttggac
     1981 ctactataga aaacgacgca ctaccaatag caaatacaat tactaaaatt attgatattt
     2041 taaatttaga taaatatgca attagttttc gcatagtccc tttaaagtca tttgcttttt
     2101 gtcctgctcc catagatttt cccataggac ctcttcccat tctactcatt agctagctcc
     2161 tcctttgaaa gttgtgataa agctatttgc tcataaactt cgcaattttt tagaagctct
     2221 ttatgtgttc ctattccaac tatttcgccc tcatttaaaa caattatctg ttctgcattt
     2281 aagatagtac taattctttg tgcaactatt agaactgtac tttcagcagt ttttgatttt
     2341 aaagccttac gaagagctgc atcagttttt aagtcaagtg ctgaaaaact atcatcaaat
     2401 atataaactt ctggattttt tgctattgct cttgcaatag aaaatctttg tttttgacct
     2461 ccagatacat ttgttccacc ttgagaaatt tcagttttaa aagcttccgg tttagaatct
     2521 ataaattctg tagcttgagc tatttcagct gctgttctca tttcttcatc tgttgcgtct
     2581 tttctaccat attttaagtt ggattctata gtaccagaaa acaatactcc cttttgaggt
     2641 acatatccaa ttttttccct taaatcatgt tgagaaacat tttttatatt aactccacca
     2701 agtaatattt ctccttcagt aacatcaaag aaacgaggaa ttaaatttat aagagttgac
     2761 ttaccactac cagtacttcc tataaaggca gttgtttctc ctggctttgc tgtaaaagat
     2821 atatttgtta atataggttc ttctgcgtct ggatacatga acgaaacatt tttaaattca
     2881 acaagaccct ttttatcttc cttaaactct tgtgcctctg atttttctgg gtcttttatg
     2941 cttatatctg acactaatac ttcatctata cgtgtagctg aaacggatgc tcttggaagc
     3001 ataatagata ccattgatat cattaagaat gacattatta tttgcattgt atattgtata
     3061 aatgccatca tatctccaac ttgcattgct ccagaatcaa cactgtatgc acctctccaa
     3121 actataagaa ctgtgataag gttcattata agcatcatag caggcatcat acatgtcata
     3181 actctattta caaaaagatt tgttttagtt aaatttttat ttgctttatc aaatctttct
     3241 tcttcatgtt tttcagtact aaaagctctg ataactggaa taccagtaat tatttcacgg
     3301 cttactaagt ttaacttatc aacaagcttt tgaaccagtt taaacttagg cataaccact
     3361 gagaataaca ctacaattaa tgataagata gcaactactg ctactgctat aatccaggcc
     3421 attgaagtat ttgtgtttaa aacctttatc actccaccta tagccattat aggtgcatag
     3481 aatactattc taagcaacat aaccatcaac atttgtatct gttgaatatc atttgtacta
     3541 cgagtaatta atgaagctgt agaaaattca ttcatttcag tatttgaaaa tgacataact
     3601 tttctaaata ctccacttct taaatttcta cctacacttg cggctactct tgctgctaaa
     3661 aatcctactg ttattgtagc taccatacta atcattgcta taccaagcat tttagcacca
     3721 gtaaataata tataattatt ttgagttttt tctgtatcaa tacctatttt ctcatattct
     3781 gaccttacat atgaaacaga gctttgtgtt atcatactct ctggcatact ttcaaagcct
     3841 ttgctcatct ctttagtcaa tgtatctaac tgttcctttg gcattgctga taacaattta
     3901 aatatatcaa catctccact ttgagtagct tgtggtggaa gtttactcat taactgagct
     3961 ttcatagctt ttacctcttt actatcgccc tcaagccctg atactattaa aatagcttta
     4021 ccaaaaataa catttaaatc atctatagtg tctttgtctt tagtatttaa ctcataaaga
     4081 gattcatctt ttagacctgg gtacttatct ttatacttaa cataatcctt tttattcagc
     4141 aaagtataat tatctagtac tttatcctta ctacttttat ccataaataa agtaatttta
     4201 tttaattcac tctctctaat cacagatgga acagagtttt ctactccacc ttgttgtata
     4261 cctacattta ctatattgga tgtgtattct ggtaaagaaa ggtcacatac cgcttgaatt
     4321 accaatagac agactattat tactattgat aatgccgatt tctttaaata tttaattaat
     4381 ttaaacatac atttcttccc ttctgtaata ttaattagtc taataacatt aaattgaaaa
     4441 acattatttt aaactttaaa aatatttttc atcttcatgc ttatgacatc ctgcttttaa
     4501 attatctcgt acttgcatta gaagtcttct aagtactatt ttttcttctt cactaaatcc
     4561 taaaaaaaac tctctttcca attccttgtt aaattcttca actttattac atatttctaa
     4621 acctttgtct gtcaaaaaaa tcctagatac tctttggtcc ttttcatctt gttttctttc
     4681 cacaagtcca gctttttcca tcctctttat cataactgta gttgtagatg cttttacttt
     4741 ccttttatct gctaattctt tttgactttg cccatctttc atctttaaag aaaaaagtat
     4801 ttggggttga ccatgatata agcctaattt atcaaaataa aaatgagttc ttataaaata
     4861 tattcgcatt aattgcaaga aaacatcatt taatttttct tcagaattgc tattcatact
     4921 tgccctcctt attttttata ttacttagtc agctaagtat tattttacta tactgcatgc
     4981 attttttcaa tatatttata aaaaattcat ataaatttca tttattaaat atttttatat
     5041 catatagtta taattttaat aaataatagt aagattttta attagtcagc tacttatatt
     5101 ttttattgta aagcttaaaa taaacctttt catatatttt caaataaaaa agttgtccca
     5161 aaataaattt tgaaacaaca tcatattcaa cagtatcttg attatataac attatgatac
     5221 tctacttatc ttcatatcca tttacaacaa catctaactt cccagtttca ggtgatataa
     5281 ttagaccatg tacaataata ccatctggaa ttaaaggatg cccttttatt aaatcaatac
     5341 tatcttttac agattcttct gcagaatcga atccatgtaa ccattgtttt gtatcaattc
     5401 cagcactacg aagtgtaagt attacatcat tagatattcc tctatctaaa attttaccaa
     5461 gtaacttatc cgtatcaaca ttacacatac cacatccatg atgccctaca accataacct
     5521 catctacatc aaattcgtaa attgcaacta aaacacttct cataatactt ccaaaaggat
     5581 gcattatagt agctcctgca ttttttatta gtttcacatc tccatttttc aaattcatag
     5641 ccttaggcaa aagctcagtt aatcttgtat ccatacaaga taagactact atttttttat
     5701 ttggatgttt tgatgtcaca tattgttcat attctttgtt ctctacaaat gatttattat
     5761 attctaatat ctcttcaagt tttctcatat gtaatatcac cctctttaaa aattttttta
     5821 ttattatatc actattaaag t
//
LOCUS       DAEMIK010000199         5811 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEMIK010000199 DAEMIK010000000
VERSION     DAEMIK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.202"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..599)
                     /locus_tag="KPK87_003509"
     CDS             complement(<1..599)
                     /locus_tag="KPK87_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2539614.1"
                     /translation="MKPELKKLLILNAPYLLFVYLFDKVGQAVRLSPGADLSGKVLSL
                     ADGFSAAFANPLPSFFPADLLIGIVGAVLIRLMVYFKGKNAKKYRKGIEYGSARWGNA
                     EDIKPYTDPVFQNNVLLTQTERLTMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKPN
                     LMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTIN"
     gene            complement(596..1072)
                     /locus_tag="KPK87_003510"
     CDS             complement(596..1072)
                     /locus_tag="KPK87_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG2539615.1"
                     /translation="MQDEVNEKVVSLAIKTSKLTAEVLQKAMKALLAKGKGQLTKAPH
                     GKITMRQLMKPGEKVSNIEITDQNIKAFDPIARKNGLDYNVKKIENGKPPTYLVSFRG
                     KDIDVMTEAFREFTAKKLGRNKKPSIRKLLSTLKDKAAALNAQRDKVKKKDREVSL"
     gene            complement(1155..1373)
                     /locus_tag="KPK87_003511"
     CDS             complement(1155..1373)
                     /locus_tag="KPK87_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBG2539616.1"
                     /translation="MTQKTGALIFDETADRYDIRFDLNDYYGGLHCGDCLEVFVRGKW
                     KPTRMEYGDNWYLVGVRASDLNGLRVRI"
     gene            complement(1394..2368)
                     /locus_tag="KPK87_003512"
     CDS             complement(1394..2368)
                     /locus_tag="KPK87_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539617.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPTL
                     DCPVLENPTADKPILENPTPDNPTSENPTQLNKELLKTNLPKKEKLNTDISNTHSIPF
                     HSLNPSPLEDAAQPPERKRKETTDAYRVYEEIIKDNIEYEHFIKHTDFEKERLDEIVS
                     LILETVCTKRKTIRIAGDDYPAELVKAKFMKLNSSHIEFVFACMSENTTKIRNIKQYL
                     KAVLFNAPNTIDSYYTALVNHDMYGGRTIQSARSKGIPDYTYNEGESL"
     gene            complement(2939..3160)
                     /locus_tag="KPK87_003513"
     CDS             complement(2939..3160)
                     /locus_tag="KPK87_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG2539618.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            3740..3997
                     /locus_tag="KPK87_003514"
     CDS             3740..3997
                     /locus_tag="KPK87_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2539619.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            4466..5704
                     /locus_tag="KPK87_003515"
     CDS             4466..5704
                     /locus_tag="KPK87_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2539620.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1890 a          934 c         1093 g         1894 t
ORIGIN      
        1 aaattgattg tattcagcac ctttatccga tacccgccgc gctgtaagag cttcccgcac
       61 tcgactaaaa ccgtcccttt cgggtcggtt acaacgtagc tgctgtgcat ttgcattaag
      121 ttgggcttca caaaaaatct tgtcttgccg ctgccgcttc ccccgataac aaggatattt
      181 ttattccttg catacttcgg ctgcttcggg cggctgttca tggtaagccg ttccgtctgt
      241 gtcagcagca cattattttg aaataccggg tcggtgtagg gttttatatc ttcggcgttg
      301 ccccaacggg cagaaccgta ttctatgcct tttcgatatt tcttcgcgtt cttgcctttg
      361 aagtacacca taagccggat aaggacagcc cccacaatac cgataagcaa atcggcagga
      421 aaaaaactcg gaagcgggtt tgcaaaggca gcggaaaagc cgtctgcaag ggaaagcacc
      481 ttgccggaaa ggtcagcccc cggagagagc cgcaccgcct gtccgacttt atcaaagaga
      541 tagacaaaga gcagataggg ggcatttagg ataagcagct ttttaagttc cggcttcata
      601 gagatacctc cctgtccttt ttcttcactt tgtccctctg tgcgttcaga gctgccgcct
      661 tgtctttcag agtggaaagc agcttgcgga tagagggctt tttattcctg cctaatttct
      721 ttgcggtaaa ttcccggaac gcttcggtca taacgtcaat atccttgccc ctaaaagaca
      781 caaggtaggt cggcggcttg ccgttctcaa tcttcttgac gttgtaatca agcccgtttt
      841 tccttgcgat agggtcaaag gctttgatat tctggtcggt aatctcaatg ttggaaacct
      901 tttctcccgg cttcataagc tgccgcatag tgatttttcc atgcggggct ttggttagct
      961 gccctttgcc tttggcaagc agggctttca tagccttttg cagcacttcg gcggtcagct
     1021 tagatgtttt gattgctaaa gacacaactt tttcgttgac ttcatcttgc atgaaatccc
     1081 tcctttcgtg gttttgttag ggtggtgtgc cgcttcgggg cataaaaata aggcgtacat
     1141 ttttcatgcc acctttagat acgcacccgc agcccgttca aatccgaagc cctcacaccc
     1201 acaagatacc agttgtcccc gtactccatg cgggtcggct tccatttgcc gcgtacaaac
     1261 acttcaaggc aatccccaca atgcaatcct ccgtagtagt cgttaaggtc aaagcgaatg
     1321 tcgtaacggt ctgctgtttc atcaaaaatc aaagctcctg ttttctgtgt cataataaaa
     1381 tcctcctttg gttttacagg ctttcgccct cattgtaggt gtaatcgggt atgcccttgc
     1441 ttcttgccga ctggatagta cgcccgccgt acatatcgtg attgactagg gcggtgtaat
     1501 agctatcaat ggtgtttggg gcattgaaca gtaccgcttt cagatattgc ttgatgttgc
     1561 ggattttggt ggtgttctca ctcatacagg caaaaacaaa ctcaatatga ctgctgttca
     1621 gcttcataaa ctttgctttc acaagctctg ccggataatc gtccccggcg atacggatag
     1681 tctttcgttt ggtgcagacc gtttcaagga taagggacac aatctcgtct aagcgttctt
     1741 tctcaaaatc agtgtgcttg atgaaatgct catactcgat attgtccttg ataatttcct
     1801 catacactcg gtatgcgtct gtcgtttcct ttcgcttccg ttccggcggc tgtgccgcgt
     1861 cctctaaagg agagggattt agggaatgga aaggaatgga atgggtattt gatatatctg
     1921 tatttaattt ttcttttttt ggtaagttag tctttaatag ttctttattt aattgcgttg
     1981 gattttccga tgtcgggtta tccggtgtcg gattttccaa tatcggctta tccgctgttg
     2041 gattttccaa taccggacaa tccaatgtcg ggggctgtgg ctgctcgtaa atggtgtact
     2101 cgatagcggt cattttgcct ttctcgtcgc gtccctgccg ccttgtgata tatccggctt
     2161 tttcaagctc ccatacagcg gtgcggatag cgtcgatact ttcccggttg ataagggaca
     2221 gccctgcaag ggtatagtcc caatcttcgg gaagtgaaag catttgcgac aacaagccct
     2281 ttgcttttaa ggaaagctcc ttgtttcgta aatggtggtt gctcataacc gtataacctt
     2341 tattcttttc cacgcggaaa actgccatag cttcatcact cctttggttg gatttgttac
     2401 gcagtagaaa cgcggttttg agggaaaagg tatcaaccct tgcctttctc attttcacgt
     2461 cctaagagcc gcataaatca aggctttttt gccccttact gcgtaacatg agggcataaa
     2521 aatagcggcg ttcctgttct cctgtgtagg agtgagaaac gccgccttat aggtggtgct
     2581 tattttattt tttcttttcg acgtgccgcg taaggtggtg tagcggtcta tccggcagct
     2641 ccgcaggtgc ttttgcatat caaggcgcaa tccgttaaaa gtagtaaatg agtagtaaaa
     2701 tcaatctgca aacctgcgaa tatgcccgta aatcaagcat ttttcaagac aatcaactaa
     2761 tttcaattaa atacgtttaa tatattaatt tttcaaagca ttaatatatt aaagttataa
     2821 tcaaaattga agttataatc tgccaaatct atagcgtatt aagtgcgtta tataacttaa
     2881 tcttttatat aaagagttaa tttttttaat ttgtaaagca gagtacgttt aaaaggtact
     2941 atttagataa tgatgtacta tctaatttta aaatatctac aatagggttc tcttgaagac
     3001 ttgctattgc ttctgcaaca gcataaacat cttcatttaa tgcctctgct tttacatttg
     3061 caaatgattt acttttaaca actgccttac ccttatcatc aaaaccagca tccaatttta
     3121 gttttatact agataaattt tttgttgaaa caacttccat aaaattacct cctaaaaatt
     3181 taatttcaaa gatttgtcga ctttctttgt tagctataat actatcatct aagataatat
     3241 cggagttaaa cagtaatttc taaagatttt ttgtgtaaat ttttatagga aaaattttac
     3301 ttttttgctg atgaaaaagt ttacaagtta tttgtgaaga tatgttctta gtgttttgaa
     3361 atagtatttg tatatattca tgcttagttg agatagtttt gtatggattt tcatatgtgt
     3421 agagttaaat gttaggtact aaagtttatg ttaaattaaa tgttaaagct tatgttaaaa
     3481 attttattaa atttaatctg taaataaaaa agttaaaagt ccaatattaa cattttactt
     3541 ttaaaccatt aaaatcttta ttaaagctaa caaagttata agttatgaga aattttatta
     3601 aagttaatac aaaaattaat tttaaagcta agaaattatt tattagattc aataaaacta
     3661 ataaatatag tttagtcaat aatgtattaa ataaaaactt tgttaagtct ataaaaatat
     3721 cttaaaggag gtgtaactta tgaagataaa agtggaggat ataccagttc aatttcatgc
     3781 aatggtagaa ataattggaa ttgataagtt catagaggtt gccaagttgt atggaggaac
     3841 aaatacttat atacctacgt ataaggggct ttttagatat gctagaaata gggatatagt
     3901 aaaacaattt aatggagtca accacaatga attggctatt agatataata tgtgtgtaag
     3961 caatattaag cgaatactca atgaaaatag tgtttaaata taataaaata atgtttaaac
     4021 ataatgaaaa tagccttgtt aaatgtaaga gcaaagacaa agaagatgtt ttataaagtt
     4081 tttttagaca aattaactaa atttactact tattaattta aatacattgt atattctata
     4141 aaaaaatttg ttaaatactg gagatatatt gagaatacaa agaaaatggt cgaaaatatt
     4201 tgttggctaa aaaatagata aaatgataca attgcttgta taaaggggaa aaataaacaa
     4261 ataaactata taaataaaga aatagcaatt aatatgacat aatatattta taaaattaag
     4321 aatataaata gatattttaa ggtattagtt gttaaattag gaaatattaa aaaaaatata
     4381 tatttatatt taaactattt tgttatttat ataaaaatac ttatctagtt actagcaatt
     4441 attagtagaa atgggggaaa tagagatgaa taagggattt tacaaattaa attcagtggt
     4501 attgaacttt gatattaaga atgatgttaa caagataaga tttaatcatt atataatgag
     4561 aaaagaaaac atacaaagac ttcaaggaac tctgccaaac ggacaatttt atatgacagt
     4621 gagaagagct gctacagatt tacaactttc tatttctact ataagtaggc ttgtaaaaga
     4681 gtttttggat ttagggataa taagattagt gtctaagggt gtaaaaggta atcgttctgt
     4741 atatagttac gtaagtatag agtttaatgg aagtttattt gatgatagta aatatatgga
     4801 acttgactgt gtatgtaatg atgaatttat tgataatagt ggcatagagt ttgattgtga
     4861 tagcaaagat aattctttta atggagatat aaatgtagaa actgtgcatt gcattaatga
     4921 gtatttgtct gatgatgatg tagagcataa ttataacaat ttccataata aagatactat
     4981 aaaggataaa gccatttcta atattagcac tatcaaaaaa tatattgatg aatcttttga
     5041 taaaaataaa tcagaaaatt ctaacttaca acaggagtca ataaatttca actttaatga
     5101 tacatgcaat gaaatagata aaagaacgaa taatagtata gaaaataatc aaaataaaag
     5161 gcctatgaga ttgagtgaca tcagtgtttt aagtgataaa ggtatgacaa ataaaaagaa
     5221 ttactttaat cataatcatg gtatacaaaa agggactaaa tctgtaacaa agaaaaaaga
     5281 atccttaaat aaatttaata aaaaaaatat acagcaaagc attataaata tgctaaataa
     5341 aaaatcaggt aagaatttta aaatcgattc tccaataagc ataaaactta tcaatgaaag
     5401 gctaaaggaa ggctatgtct tagaagattt ctacaaagtt attgaggtaa aagtggctaa
     5461 gtggaaaaat accataatgg agatgtatat aagaccagaa accttgttta gctataagtt
     5521 tgaatcctat gtaaatgaaa acatatctat gaaaagtcaa agtaatgttc acccatgtaa
     5581 agaaggcaat agtaatgata gcagtaatta tatacattct gattatacaa catatgatgg
     5641 cagtgtgcgt gagagaaaaa aatatgagaa tgtaagatac tggaatccta tatgtgagat
     5701 ttaattagtt taagaatgta tttatgataa ataagatatt aatagagaat agtgagctat
     5761 agcaaatgat atatcaatag ttggtagtaa gttatattaa gtaagatatt a
//
LOCUS       DAEMIK010000200         5675 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEMIK010000200 DAEMIK010000000
VERSION     DAEMIK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.203"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(194..526)
                     /locus_tag="KPK87_003516"
     CDS             complement(194..526)
                     /locus_tag="KPK87_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG2539621.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(770..1411)
                     /locus_tag="KPK87_003517"
     CDS             complement(770..1411)
                     /locus_tag="KPK87_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG2539622.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(1392..1685)
                     /locus_tag="KPK87_003518"
     CDS             complement(1392..1685)
                     /locus_tag="KPK87_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2539623.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2078..2137
                     /locus_tag="KPK87_003519"
     CDS             2078..2137
                     /locus_tag="KPK87_003519"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG2539624.1"
                     /translation="MTEYIYYYNNKRIKKINLA"
     gene            complement(2653..3405)
                     /locus_tag="KPK87_003520"
     CDS             complement(2653..3405)
                     /locus_tag="KPK87_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG2539625.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3464..5119)
                     /locus_tag="KPK87_003521"
     CDS             complement(3464..5119)
                     /locus_tag="KPK87_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539626.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT         2030 a          883 c          484 g         2278 t
ORIGIN      
        1 taataatgac aatatactat tctcaataca atctatgatg taaataattt aaaactgcta
       61 tattatttaa aaatttatat ttatatatgc aattctaaaa acttaataag aagtgaactt
      121 agaaaataaa gcttaatttc aaagacttaa atagactatt attattagtc taaataaact
      181 acgtctatca aaattatata ttaaattctt ctaaaatttt tattgcaaat cttgcgtttg
      241 gatatgtaat agacccgcca ccaattacac ctatttttat agcttctata atttcatctt
      301 tacttgctcc agaattaata caaccttcaa tatgataaca aatacactca tcacatcggc
      361 ttaaaattga aatagctagt cccattaatt ccttattttt tttacttata gccccttctg
      421 aataagtctt atcatcaaga cttccaaata ctctaaaaaa ctcatctgtt tcgcttaatt
      481 ttttattgta aaatacccta tattctttca tttctctagt attcatcttc tacctcctat
      541 gtttttctta taagaatatc acaccgtact tatattttca atattaaata caaattttca
      601 ttttatgttc attagcctaa tttatattat tttttgaaat ctcaataagt tatcatataa
      661 gtaagatttt ttcagaacta aaatagaaat cttagtaatt tctttattat ctatctcagt
      721 tctgaaatat attattctta tccaaacatt attatagtga ttaattatat tagttatttt
      781 tacctgaaat tttatacatc aaataagaat atataaatgg tagtattaca attacaaaga
      841 ttacaaagat attataaaaa ggaatattaa taaatgcatt aataattaat aatattccac
      901 caataagcca aatccatcca gctaaatggt gtgtcttatt ccaattatct tcatcattaa
      961 gagtccatgg taaaattatt cctactgagt aatttcgttt acatttagga agatagtttc
     1021 caaataaaat aactattatt cctaaaatta atcctgtata aaaattagac tccaaactaa
     1081 ctcctgttga atataaaacc atcattattt gtataattac tgaaagtata gcaactcccc
     1141 attttgataa attttgtata acatttgaaa aattagcttt cttaggctca ttctcaagtc
     1201 taaaccatgt atacaaatgg atacatagca ttaacaatgg tacaccaaaa gctgctataa
     1261 cctttggagc ataattatct ggatttccat aattatcaaa atgaattgca acttcatttg
     1321 gtaatctatt ataaaaatat attgatacaa taaatggtat aaaacatatt aaagtagata
     1381 aaataaattg tttatttatc gcttttttca ttatcttttt ctcccttctc cataaattgt
     1441 gaaaaccaaa gcatgacctc ttcgaaaact gaaatattta tttcgtagaa aataaagttc
     1501 ttatactttg tttcaaatat gagtcctgcc ttttttagtt gagatagatg ataagaaatt
     1561 gtagcattcg tcatattaaa tttttcacta atttctcctg ctgacatttt attctccctc
     1621 aacataacca atatttctct tcttataggg tctgaaagag ctttgaatgt ttctggaaac
     1681 cccaacacca tcacctcgct atttagaatt tcatctaaat agaatataac ataacatcca
     1741 ttttatttca atagctattt agaaattttt ctaaatagtt atttctaaat atttattgaa
     1801 aatttttata ttaagattct caatttactt aagtcgcttt tatttaatct tactttatct
     1861 aattttttta acttaacaaa agtgtattgc accataattg ttaaagcaaa aacctaatga
     1921 ttgaggtact tatatatata acaatacttt taattttaaa ttaaaagtag ctaaacccaa
     1981 tttaaaaagt agttaagtcc tatttaaata gaaaagaatg aaacgagaaa tatactgtat
     2041 aaaagtacat aaaaacatct actaattaga tacaactata acagaatata tttattatta
     2101 taataacaaa agaataaaaa aaattaattt agcataacct tgttaaatat agatttgaat
     2161 atgaatttaa agttatgtaa aattaaaaat acaggaatga aaattccatt cctgtataaa
     2221 atctaacttt tgagtctcag tacaatctta ttttgagaca atcttttttg ttttatattt
     2281 tcttatattt aaaatataac gcatatctta tgcaacatat tcatattttt ctttatattc
     2341 tttcatcgca tcattccaag cgtcttgtac tgttacacct cttttattag caattttttg
     2401 tgccagttca attatcttca tagctcttct tgatatgttc ataaacattc ctcctacaaa
     2461 ttaccatata tttcatatat ataaatcaag gttatatttt tatgatatat aatccttcta
     2521 ttgtaagatt acagtttata tgtgtattat atatgaacta tattagaaga taattagaat
     2581 ttttataaag ctatatgatt agattaaata taaaagcttt acaattactt ataaatttaa
     2641 aaagtctata agctaaacta atgtttgata taccaatttt acgaccataa ataatgccat
     2701 tactaaaaat attggtttta tcatctttga acctttcttt atagcaactt tagcacctac
     2761 ataagctccc attatcatca ctaatgcaaa cactatagca attttataat ctacttgacc
     2821 atttatcatg aataataaca atgaagttat attacttgtt aagtttaata tttttgtatt
     2881 tgcagaagca tgtaaaaaat catatccata tatttttata aatccaaaag tcaaaaaagt
     2941 tcctgtacct ggaccaaaaa aaccatcata aaaacccatt ataagagcca taagcatccc
     3001 taatcttaaa tttttcttat taacagcttc aaaattatct tcttgaccta aatttttaga
     3061 aataaaggtg taaatagcca ctattaaaat cataaccaca accaaaacat ttaatatctc
     3121 ttcacttatt gataatacac atctcacacc tagaacacta ccaataatag taaatggtac
     3181 taattttttt aaaaggttgt tattaatttt tccagacttt gcatatctat aggcactact
     3241 tatacatcct gctgatgctg caaatttatt tgtaccaatg gcaagatgta ctggcactcc
     3301 aattgccata agaacaggca tgcttataag tcctcctcct cctgctatag aatctacaaa
     3361 agcagctaaa aaccccccta tacataaaaa aataactgaa aacataaaat tcctccatca
     3421 ttctctttca atttttctta caatatatat tatataatct tatttaatac ttattatcca
     3481 gcatattctt tctatattca tttggagtta tactattaaa cttcttaaat atttgtgaaa
     3541 aataactact gttattaaaa cctactttct tacaaatatc actaatactc atgcttctat
     3601 ctaacagata ttttttagat tcttctatcc taagaagatt tatatactca gttattgaaa
     3661 ctttatttct ttcattaaaa atagaactca aataatttgg acttataaaa aaaacatctg
     3721 atatactatt caaatttata ttttctttat aatatttttt tatatattcg tttatatttt
     3781 caattagatt tcctttatct tttctccaaa atgctctatt cttatcaata cactctttgg
     3841 aaaattcaaa taatacattc cataaatctt catcactgtt tacaagtgat atttttttat
     3901 tatatttatt tcttaaataa gatataagtt ttaaatcctc taaatctcta agcactgttc
     3961 tagacaatac aaatatcatt tcctgaatag tatctctaac atcattatat cttaatttgt
     4021 ttgtatctat tagattataa acaccttcta aattcatatt tttaatttct ttttcaatat
     4081 aaagttctaa ttcaaaggga tattcttttg aagaattatt gaacatatct atcggattaa
     4141 taatatactc ttcttctaat aatctactat ttgctttttt aagtgcctct tcaagtctta
     4201 tattttcttt tctactcttc aatatctcct catcttttat actaagttga ttttcaaaaa
     4261 aattcctctt agctatttcc tgaataaatt cagacatcat taataaattt tcttttaata
     4321 catataatct acttttagga tttaatggaa attcattgta aattttgttt atgtatttta
     4381 tatcaatacc agaactactt gataattcag ataaattttt atctatatca atagacttat
     4441 atactataaa attaccacat tctatatatc caactatatt ttcttctaat actataggta
     4501 tctctaaact ggaaacacca tactcacaat ggtacacttt ttcaaagtta tcattattta
     4561 tgcttttaac aagtttttta gtacactgtt ttatattttt acaaatatcg caaaatttat
     4621 tttctttctt agtctcaaat atttctttat actttaagtc atatacagat attctaagct
     4681 tcaatatatt agatatttga tttgcaagat tttcaattat gtttacatta actaactcct
     4741 gtatattgtt ttcagaaaat actataggta tttgtctcaa actcgaaaat ttatgaggaa
     4801 cataagtaat taatattact aaatcaattt taccagtgtt tataatttca tgttctgaat
     4861 atggtgggca ataaactata gatgtttcct ttatattgac ctttttacca tcaattattt
     4921 gtattccttc tccctgaatc acatataaaa gctgttcttc acttaaatga aaatgctttt
     4981 cttggtagtt tcccggaaaa aatcttacaa ttccagcact aagtctattg aattgtgttt
     5041 ttgatggttc gtgtatccac ataacctctc cccaatcaaa tttttgtact tcattatttg
     5101 actcactaaa atactccata agctcacctc ttagtttttg tctaaatatt ttttcttccc
     5161 ttcttatatt aaaataatat catcatcatt aactttcatt actataaaat catcatactc
     5221 ttttaatact cctgtaattt tagttgcact tagtttacta caattcagtt ttttaatata
     5281 ttgataaatt ctataattac ttttttattt tgtaaaatca gtgttttccc aaaataatta
     5341 tatctataaa tgaaagtatt tttactgctt acatcaatat atgttatcat aattttcctt
     5401 tttattgcat atataaataa ctgtatataa cattgtccaa tactatatag aaatacatct
     5461 aagaaagaat attattaaat tgttccaatt agtattattt ttcttataga aataaaaaaa
     5521 ctgttggagt cttttagaaa aattattttt tacatacctt ttttgcttag atagtctatc
     5581 ttcacagcta ttttgtatat acactcttct agttcatgat tttattttaa tatcttgctt
     5641 atattgctga aataattgaa ttcttttatc tatca
//
LOCUS       DAEMIK010000201         5562 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEMIK010000201 DAEMIK010000000
VERSION     DAEMIK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.204"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..298)
                     /locus_tag="KPK87_003522"
     CDS             complement(<1..298)
                     /locus_tag="KPK87_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539627.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRH"
     gene            complement(654..1139)
                     /locus_tag="KPK87_003523"
     CDS             complement(654..1139)
                     /locus_tag="KPK87_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2539628.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(1222..1315)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1671..2054)
                     /locus_tag="KPK87_003524"
                     /pseudo
     CDS             complement(1671..2054)
                     /locus_tag="KPK87_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      2280..2377
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2432..2608
                     /locus_tag="KPK87_003525"
     CDS             2432..2608
                     /locus_tag="KPK87_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539629.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            2834..3034
                     /locus_tag="KPK87_003526"
     CDS             2834..3034
                     /locus_tag="KPK87_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2539630.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   3397..3627
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3463..3493
                     /rpt_unit_seq="actttacataccacatagttgatataaaacc"
     gene            3853..4035
                     /locus_tag="KPK87_003527"
     CDS             3853..4035
                     /locus_tag="KPK87_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG2539631.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            4106..4951
                     /locus_tag="KPK87_003528"
     CDS             4106..4951
                     /locus_tag="KPK87_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539632.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYSLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(5175..5339)
                     /locus_tag="KPK87_003529"
     CDS             complement(5175..5339)
                     /locus_tag="KPK87_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539633.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         2016 a          725 c          581 g         2240 t
ORIGIN      
        1 tatgtcttct tgcgttttgt cttttatatt tatttatagt ttgatatcca tactttttag
       61 ttcttatact tcgttttttc aactttactt tacttttatt atatttccta cttttatgtc
      121 tacttttaga tttaatagtt ttaaatttag tattatcata tgtataattc tcttttattg
      181 aagaaatgta tacactagac tcaataatag cttctgcttc tacttgtaat ggatttataa
      241 tgctgattat aagtagaaaa gatatagcta tcccaatcaa ttttttcttc ttattcatat
      301 tttccccctc ataaaaattt ttaactagtt cattataata tatatataac taaaaattac
      361 aaattttttc aagtttctta tatatcttat tatctttaat attattttta ttatcataac
      421 ttttactttt tttaagattt ttcaaagtag tattaattta tgatacttgg tgtataaagt
      481 ataagttaaa tctactttca attaaattta ccatttataa ttactttata ataaaaataa
      541 attagttaat ttattctctt tagttctgtt ataatgtttc tacaaaaaag aatcccatta
      601 atgggattct tttttgtgat ttattaatat atttaaagct tgctagtatc gtattataaa
      661 cttaaatgct cttctatgta ttgattatat acatatccag aatatcgttt ttcattttta
      721 ttgatattaa ctcttgccca ttttccaacg ctataacata cttcaacttt aaaaccatca
      781 gggattgtag ttataacttt agattctaat gttctatcat ttctaagttc taaacttaat
      841 ccatttgttc ttatatttcc tatgtaattc ttttttctat tttttattgt atcattacta
      901 ttgtttttag cattacttaa tctttctaaa tatttataaa gtaaccaacc tttgtcataa
      961 ttactctcaa cttctgccca tattccatct acacacttta acttaacaat tttaccttct
     1021 ggtatacttt ttaatttcaa actatttatg tctggttttt ctcttaaatt caatttaatt
     1081 ttagtcttaa aattgatgct agatttgcga aaatttttta acattaattc agatatcatc
     1141 ttaagccctc ctcttgccaa atattaagtt attaaaatat ataactctaa aaatactttt
     1201 aacaaaaaga attattatct ttttggcaac tggctgacat gacctttatt acataaagat
     1261 tttagtccct atagttttgc gtcattagat ttatctaata ttgcctatat ctgaataata
     1321 acataatttg aacaattaaa aaataatatt acctcaaatt ttatattatt taaatataaa
     1381 ttaaattttt aattaataat aatcaaaaaa tatctttata tttattcaat tactgttctt
     1441 tatagtcaat ataaaagaca gtaatttatc tatagtgtta cttatatttt atatatatgg
     1501 ctatttttca acattttaca atatatataa catttatttt tttaactttt gtcagtaaaa
     1561 tgtcagcaaa acactttatc cacaattttt atataagttt tccacaactt atcaccaata
     1621 ttatccacat aaaaaaggta gcacttatat tattagctac cttttattta ttaatcttta
     1681 tatttccagc tttttatcca acttcctatt tctattattt tttcttcagt tatttcttct
     1741 ccatcatgta attttgaaat taattctgat aaaggattgt tatgtaaacc tctaaaaact
     1801 ctttttccct ctattttgag atattctctc tcttttatta atactgtata gtgtacatgc
     1861 ctcccaattc tctatgatat taaaaaacct tttttggtta atctagctaa aagtataatt
     1921 gtagttgtat gcttccaacc atatgttttt tctatttcat ctgcaacatc ttttgatatt
     1981 acagttttat aaccagtatt ccatatgcat ttcataattt ttaattctgt atctggtaat
     2041 tttttattta gcataatata ttccttcttt caatttatgt cacttcttca aatttatcta
     2101 gcttaaatta taataggagc tttttagaac aaaaactata tttaattttt accaggtact
     2161 ctacgtgttt agtctgtttt taagtgtaaa ataatattca tatctataaa tttatttaac
     2221 tttcaaacaa tcctgttgaa taataactaa ttctattgta ttattttagt tgtagaataa
     2281 aactaaatcg gcaaaactag agaaatttag tgacgcaaag ctatagggac taagacttat
     2341 ataaatatct tatgagttat gtcagccagt tgccaaagag atagtgttct ttttgttttt
     2401 atgaaagttt tttaggggga taataattta tatgtttgga aataaaatgg ataaatgtac
     2461 acatgtattg actgcttata ttagcagttc gtatgattat tgtaattttt tagatacaca
     2521 gttagatgat tttatattag agtacggaga aaatgtagta gagtcttgtt tacaccaagt
     2581 aatggtattg gtaagtaagt ataattagat agacattgat ttctagaaaa ccctttttag
     2641 ttttatctac atttatatta atatatagca atgtaaaacg tttaaatata tttttaatat
     2701 taagaataat taaaatgtta actaaaaaat ataattaagt agacatttct attaatctat
     2761 gatacaatag tatttgtggt aatacacaat taaatcaagt atatcttgat ataggttaat
     2821 aggagtattt ttaatgaata atggaatagt gaaatggttt aataatgaaa aaggattcgg
     2881 ttttatatca atggaaggta gagatgatgt gtttgctcat ttctcagcta tacaaacttc
     2941 aggatttaaa tcattagaag aaggtcaaca agtaagcttt gatatagtta aaggtgctag
     3001 aggtcctcaa gcagaaaata taactatatt ataatatata gatttttcgg gaaagactct
     3061 ttttagagtc tttttttatt gttaatatat gtaagataat cattggtgaa gtaaataagg
     3121 tatattgagt aatgttcttg atagtttata tgctaatgaa tttcaaaaaa ataagcactc
     3181 ctataaaaag agtgctctta atgtaaatat aaaccttcat tggtccagcc taataccccg
     3241 tattctccaa gttctaactc ttttttatat acattatcac tttcagccat atctaacaca
     3301 atcatttctt ctttagaatc aatttgtaaa atatgaggac tgtgtgttgt gacaattatt
     3361 tgtgtttttg ggagaattgc ttttaatgta ataaaaattt tacataccac atagttgata
     3421 taaaactatt aagttttcta tgtttatatt catattcaaa caactttaca taccacatag
     3481 ttgatataaa accttagtgt ataaacatcc tttaaggtca ataggctctt gtctttacat
     3541 accacatagt tgatataaaa catgtagttg ttactttcca gctacactca tgctccactt
     3601 tacataccac atagttgata taaaacccca aaataaactt agcatctcca atgtttacac
     3661 atatacaatg ctcttaaaat tgcagtgaac gcgcaatagt gcaattgata atacttatta
     3721 tatattctca atgccttata tttcaactgc taaacctcaa tttatcacaa atatcgctca
     3781 ctgcaaaatt tctatatttt tattatatca taacatataa ttaatatttt caattaattt
     3841 ttacatttaa ttatgtttaa attctataat aatgcaactt taatgtttct ttctggaact
     3901 aatataaaaa ctatttcaga aagattaggt catgcagata taaaaataac tatgaatagc
     3961 tactctcagt ttttagaaga gatggacaga gaagcatttg aaaatttgag caaaatacta
     4021 tttattgtat tttaaagaag atgacttatt caatatttga tgctagaata taattaataa
     4081 aaatttaatt ataggtgatt gtattatgaa tattattaaa agcataactt tactcttaac
     4141 attattaatt gtgatattaa tgacaggctg ttccccaaga gaaagtgcta gtgaagttgc
     4201 aaaaaaatat attgataaga tgaactctgg tgactatgaa acagcatata gtttattaag
     4261 taaagatagt aaaaaaaaaa ttagtataga aaatcttgaa gaatttcaaa atattttagt
     4321 tagtacaaaa agaataatta gttataaaat aggtaaagaa aaaatcataa aaaaatataa
     4381 acatgatgga aaagaatata aaaatgttgt taaattagaa gaaactttta atataaaaaa
     4441 ccttctttat aaaaaagatg attctctaaa agtaaataga tattttgtag ttgaaaataa
     4501 caaatacaag ctattattgt atgaaagttt caaaaaagaa tttggtatta atagtatgga
     4561 ccttgctcaa cttaagctta atgattttgg actaaaatac tttaaagaag ttgaccttat
     4621 actaaaaaaa gctattgcta ttaatcctac tgattcaaag ctttattatg cccaagcatg
     4681 taattatatg aattttgatg gatttcctgg ttattcagat acaacatata atgctttaga
     4741 ttcaattaat aaatctttaa agtatttaga taaaaatgat tctgactaca aaaaaaatgc
     4801 aagtaatata tatagcttaa aaggagttat tcttatgttt cacaaaagat ttgatgaagc
     4861 acaacaatat ttaaataaag ccttatactt taacaaagat aatgaagacc caaaatataa
     4921 tctaaataca ataaaaaaac atctaaacta gatatttaat ttttgaattt ataaaatatt
     4981 tttttatgta taattcagca ctgaatcata atctctacta aactaattac aagtttaaat
     5041 tataaagact tatttctata taaatattac tctataacaa taagtttctt atttaaattt
     5101 taaaatatta tctaaaaatc ataaaaaaga aggtataaag agctaccctc aagctcaata
     5161 taccttataa atatctaaac ttcatttttt actaattttt ctatatcttt gataatatgt
     5221 tctttagatt tttttccagc tatggcatca aaaattgcaa cagccttttt ctctccaaac
     5281 ttttctgcta catcattaaa gtctaaaaat ctaacacttt cttttggttt accaaacata
     5341 taacacctct catacatttt tatatattta ttataacttc tatagactta tttttcacca
     5401 ataaacgtta gacaaattag tccctttatt tttttaaact agctttacct tcaaaattct
     5461 taacaaaaac agtttattct actctataaa taaaaataga caatttaatt tgtaggaaac
     5521 tgataataga atacagtaaa taaaactttg tttaatctat ct
//
LOCUS       DAEMIK010000202         5556 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEMIK010000202 DAEMIK010000000
VERSION     DAEMIK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.205"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            17..1129
                     /locus_tag="KPK87_003530"
     CDS             17..1129
                     /locus_tag="KPK87_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2539634.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1337..1813)
                     /locus_tag="KPK87_003531"
     CDS             complement(1337..1813)
                     /locus_tag="KPK87_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539635.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2012..3637)
                     /locus_tag="KPK87_003532"
     CDS             complement(2012..3637)
                     /locus_tag="KPK87_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2539636.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(3975..4994)
                     /locus_tag="KPK87_003533"
     CDS             complement(3975..4994)
                     /locus_tag="KPK87_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG2539637.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(4991..>5556)
                     /gene="mocA"
                     /locus_tag="KPK87_003534"
     CDS             complement(4991..>5556)
                     /gene="mocA"
                     /locus_tag="KPK87_003534"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG2539638.1"
                     /translation="NAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQVV
                     VVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFIDN
                     LYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNSSK
                     IKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         1887 a          955 c          578 g         2136 t
ORIGIN      
        1 gattggtggg ttatatatga atgcatttat tagaaaaaga aataaaaact atgttgttta
       61 cctggaattt gaagatgaaa agacaggtaa aagaaaacaa aaaaatatgg gtacttttga
      121 taaaaaaagg gaagcaagta aaagactaaa tgaggtaaaa gaaagcttat ataatgatgg
      181 ttttttagta ccaagcgaaa ttacagtgtc agaatttcta ttagattttc taaataaata
      241 tagtgaaaat atatctttag ctacatataa taattatgtt agtatttgta agaattacat
      301 taatccatct attggaaaat ataaaataca agacttacat ccggttcaca tacaaaacta
      361 catagataaa ctatcttata aactaaatcc tcaatcaatt aagattcata taaatatatt
      421 gaaacttgca ataaaaagag cttatagact caaattaata aaagaaaatg ttatggatag
      481 tatagaggct ccaagatata aaaaatttaa aaatgagatt tatgatagag aacaaatgat
      541 aaaattatta gatttagcaa aaaatacaga aatggaatta cctattaatt tggctattgg
      601 attaggtctt agaatttcag aagttttagg tctaacttgg gataatattg attttgaaga
      661 aaatacaata acagtaaata agataacttc ccgaattaat ggttctgtta ttctcaaaga
      721 gccaaagaca gaaagctctg ttagaaaaat atcttctccc aaagaattaa tgtctttatt
      781 aaaagaatat aaaataaaac agaataaaaa tttattaaaa tcttccatta gaaataataa
      841 taatttgttg tttttcaata agaaatgtga acctattgct gaagatgtta tgagtaaaaa
      901 atttaaaaga tttttagaaa agaatgaatt acctcatatt agattccatg atttaagaca
      961 ctcacatgtt actttactta taaattctaa agtacctata aaggttatat ccgagagagt
     1021 aggacactca aatataagta ctactctaag cgtttattct catgtactta aagaaatgga
     1081 taaagaggct tctgacaaaa tttctgaaag tttatttaat gctaattaat attaatttat
     1141 cttagcttta aattagctta gcttattgag atagattcat taatattaaa tgtatctatc
     1201 tcaaattaat aactacttaa ataatctgtt ttattttaat ttatcgcctc agttattata
     1261 cttttttacg aaaaattata ctttacaata aaaaagatat tcccttatag aaaatatctt
     1321 tttaaatact atattattat atacttgcta acattggaat atctaacatc ttcttcatta
     1381 catcatctac tttttttgct ttatattcat tgtatccaat taaatatcct ttttcatatg
     1441 aactttttaa aatctctttt tcatataaat tatcagattt ggcttctgtg acacttaaat
     1501 tataaagccc tttacaccca tcttcataac ctttattata ccctttttct gcaaaatagt
     1561 ttttatgatt tttttcgtta tgtactgaag atattgttac tccagaattt tcttttgtac
     1621 tagcataagg acaaaaacct ttattgtgtt gatgtgctga atgtcctcca caataataac
     1681 gtgttaactc tgatttattt ttattatgat gacctcctga tgaattatgc cctcctgagt
     1741 gagcatatga aaaattccct acactaatca tagctaaact caatactaca gctactaacc
     1801 ttttcttaac cataatctat tccccccaaa atgttatata tttttattat atcatctact
     1861 atattatatt taaattctta atatacaaaa tattactcta ttctgctaat aaacatgttt
     1921 aaacaacata aaaaacctcc taatatttga ttaaaatttt acacactcat gcatagcatg
     1981 tctcaaatat taaaaggttt tatactaata attatttatc ttttccatac attttagcta
     2041 attggtcttg tatctcttta ctttctaaat actcatcaaa gtccattttt ctatccatta
     2101 ttccatttgg agttatctct ataattctat ttgctatagt agatataaac tcatggtcat
     2161 gagaagtgaa taaaagtact cctgggaact tttcaagtcc tttatttaca gaagttatac
     2221 tttctaggtc taagtggttg gtagggtcat ctaatacaag tacatttgca tttgaaagca
     2281 ttagcttact aagcatacat ctaacctttt ctcctccaga tataacttga gcttctttta
     2341 atgcttcttc acctgagaat aacattctac ctaaaaatcc tcttataaaa ctttcacttt
     2401 tttcttcaga gaactgtcta agccagtcaa ccaaagaata ttctactccg tcaaaaaact
     2461 tattatgatt ttttggaaga taatcttgag aagtagttac tccccattta tactctccac
     2521 tgtctggttc aagttctccc ataactatgt tgaaaagtgc agtagtagct atttcatctc
     2581 ccataaatac tactttatca tctctatcca atctaaatga cacattatca agaaccttaa
     2641 ccccatctat agttttagtt aaattgtgta cttctaaaac ttcattccca atttctcttg
     2701 caggtgtaaa tcctacatat ggatatcttc ttcttgatgg ttgtatatct tcaacttcta
     2761 atttctctaa ctgtttcttt cttgatgttg cttgtttagc ctttgaagca ttagaactaa
     2821 atctagctat aaattccttt aattgagcta tcttttcttc agtcttttta ttttggtcct
     2881 tagccaattg taaagccaat tgacttgact cataccagaa atcatagttt ccaacgtaca
     2941 tttgtatttt accaaaatct acgtcaacta tattagtaca tatttgattt aggaaatgtc
     3001 tatcatgtga tactattata actatagatt cttctaaatc cattataaaa ttatttaacc
     3061 agtttattga tttaaaatct aagtggtttg taggttcgtc cattaatagt atttcaggct
     3121 taccaaaaag tgattgtgct aacaatacct taaccttttc cccaccaact agttccttca
     3181 tttgtttgta atgcatatct ttcgttatgc caagacccat aagtattttc tcagcattag
     3241 tttctgcatc ccatccgtca agctctgcaa actcaccttc aagttcggca gcttttatcc
     3301 catcttcttc attaaagtct tccttcatat acaatgcatc tttttctttc attatgttcc
     3361 aaagtctctc atgacctctt ataacaacat ttaaaacagt ttcttcttca tattcaaagt
     3421 ggtcctgttt taaaactgac attctttctt tatcagttat agatatacta cctgtattag
     3481 gctctatttc tcctgataat atttttaaaa aagtagattt tcccgcacca ttagctccta
     3541 ttattccata gcaattacct ttagtgaatt ttaagttaac atctttaaat agttccttat
     3601 caccaaatct aagtccaaca cctgtaactt gtaacattaa tatattttcc tttctttttg
     3661 ctcataattt attttatcat aaaactttga taacattttt attatatatt atcacgtaaa
     3721 tagtatattt tttatactaa atttgtgaaa tatttattaa aataatacta aaaattgtaa
     3781 atttataaaa attaacaatc tcttatatta tacttataaa gaaatcaaat tgcaacttta
     3841 ttcattcttt tacacattga aaataagtta ttataatttc taaataaata tgcttttttt
     3901 aaaacatcat tctagctgtt ttgtttcata tttaaaaaga gtacttaatt tgtaaatact
     3961 cttttttaat aactttatat tttagcaaaa ttacatgcag gataagtaca tacatcacag
     4021 ttcatacaaa ggccaccatg accatatcta gctatatcat acttatcaag cttttcatca
     4081 attaaaaccc tagataaaac tatatcaaat actgtagttt tgtgatacat tgcgcatcct
     4141 ggtataccca taattggaac ttctccataa taagcaagta aaaacatagc tcctggtaat
     4201 attggtgaac catatgtcac taaatcagct ccagtttctt ttatagctgt tggcgtaaca
     4261 tcatctgggt ctacactcat cccaccagta caacagataa gttctgctcc ttgacttata
     4321 aattctttta ttgcactctt tataatctct ttatcatctg ggcatatagt ctgtcctata
     4381 acttcacaac caaagccttt tactttttct tctattactg gtccaaattt atcaacaatt
     4441 ctgctataaa acacttcatt tcctgtagta acaattccta ctttcttagg tttaaaaggt
     4501 actacattta ctatttttct attaccaact actttttttg catcttctaa ttttttctca
     4561 tctataacta aaggtatcac tcttgttcca gctactttta aacctttatt tactataaaa
     4621 ttattatgaa gagtagccat cattatttct ccaacacaat tcaaatcaaa aagtgcatca
     4681 acatctatct ttaatagacc atcacatgct gctataaaat ctattttccc ttcttttatt
     4741 tcactaaaat ctaaattttc tccagcagtt agttctttta aaaacaatgc tccttcattt
     4801 tcatgaacca ttccttctga cttttcccat acatacaaat tatctttacc aagagataat
     4861 aacactggaa tatcttcttc ttttactatg tggccctttt taaattttct tcctttaaat
     4921 tctccaggta ttatttgagt tatatcgtga cataaaactt gtccaacagc atctatagtt
     4981 tttattagct tcataattta cccccaattc tctcataatc catttttgta tcgatatcaa
     5041 ataacatttc tttaggaact tctacatact ttatttttga tgaatttttt aaaatggttt
     5101 tacctttttc atcttcttta agtgccaaca attcttcttt cttactatat gggaatatga
     5161 ctggatttcc acacatatct ttgtatcttg gaattactat aaaatctcta tctaatttaa
     5221 aagtatttat aattttatct atatataagt tatcaataaa tggctggtct cctacaaaga
     5281 acatataacc atcacatttt gagctattta aaactcctaa ttttatagat tcactttgac
     5341 ctatatttgc atttttatta tctatatatt caaaaccata tttattacac aataacaaaa
     5401 cctcttcata ttgacttact actacaactt gatgaaaatc aatcttgcta acttctctaa
     5461 atacatgttc tattatagaa cagcctttat aatttaataa aagtttattt gctcccattc
     5521 ttctggcaaa gcctgatgcc attatcactg cattta
//
LOCUS       DAEMIK010000203         5524 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEMIK010000203 DAEMIK010000000
VERSION     DAEMIK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.206"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            131..2632
                     /locus_tag="KPK87_003535"
     CDS             131..2632
                     /locus_tag="KPK87_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG2539639.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2792..3460
                     /locus_tag="KPK87_003536"
     CDS             2792..3460
                     /locus_tag="KPK87_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539640.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3444..4721
                     /locus_tag="KPK87_003537"
     CDS             3444..4721
                     /locus_tag="KPK87_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG2539641.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5122..5343
                     /locus_tag="KPK87_003538"
     CDS             5122..5343
                     /locus_tag="KPK87_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539642.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         2327 a          558 c          983 g         1656 t
ORIGIN      
        1 tgatttaata tacctatagg gggtataata aaaatataga tttctaaata agtaatttat
       61 caatattaca tatcaaaata aaacgttaca aattagctga aaatatattg aaagaaagga
      121 agattttatt atgagttcaa ataaagtggt agaaagttat aaaattacag gtatgacatg
      181 tgcagcttgt gcaaaggcag tagagagagt aactaagaag atggatggag tatatgacca
      241 aagtgtaaat atagctactg aaaaacttaa aatagaatat gataattcta aggttaattt
      301 tgatgatata aagcaggttg tagaaaaagc aggatatgga attataaagg aagaaagtaa
      361 taaaaaaatt gatatgaaaa ttgatggaat gacatgtgca gcttgtgcaa aagctgtaga
      421 gagagtggta aaaaaattgg atggagtaga aagtattagt gtgaatattg caacagataa
      481 agctaatata gactatgacc catcaaaagt aaaattatct caaataaaag cagctattga
      541 aaaagctgga tataaaccaa tagaagaggt aagaaataag gttgatgtgg atgaagataa
      601 attgagaaaa gaaagagaaa tgaagtcttt atttgttaaa tttatagttg caatagtatt
      661 tgcagtacct ctgttttata tagcaatggg acctatgata ataaagccta tagggccatg
      721 gcctttgcca gaaattataa atcctatgac aaatactttt aattatgcat taattcagtt
      781 aattttagta ataccagtaa tgatagctgg ttataaattt tatataaatg ggtttaaatc
      841 acttttttct ttaagcccta atatggattc tttagttgca ataggaacat tagccgcatt
      901 tttatatagt ctatatacga cactacaaat agccaatgga caaattcaag gtatgcacca
      961 tcatcagttg tattatgaaa gtgcaggaat aataatagca cttatcttac ttggtaaata
     1021 tttagaatca aaatcaaaag gaaaaacatc agaagcaatt aaaaagctta tggggctaca
     1081 acctaaaact gcaatagtgt tagttgatgg taaagaagtt gaaactccta tagaagaagt
     1141 agagatagga gatatactat tagtaaaacc aggaactaaa atacccgtgg atggagttgt
     1201 aatagaagga tatacatcag ttgatgaatc aatgcttaca ggtgaaagta taccagtaga
     1261 aaagaatgtt ggaagtaagg tgacaggagc tagtattaat aaaaatggtg ttattaagtt
     1321 taaggctgaa aaaattggtg gagatactgc tcttgcccaa ataataaagt tagttgaaga
     1381 tgcacaaggt acaaaagcac ctattgctaa acttgccgat actgtatctg gatattttgt
     1441 accaatagta attgcaatag ctgtagttgc atcattattg tggtttttaa taggaggaaa
     1501 agatatagta tttgtactta ctatatttat atcagtactt gtaattgctt gtccttgtgc
     1561 attaggatta gcaacaccaa ctgctataat ggttgggact ggaaagggcg cagaaaatgg
     1621 aatcttgata aaaggtggag aggcactaga atctgcacat aaagtaaata cagtgatatt
     1681 tgataaaaca ggtactataa cagaaggaaa gccaaaagta acagatatag tattaaataa
     1741 taatgtaaaa gaagaatatc ttatcaagat tgcttcaagt gcagaaaaag gttcagagca
     1801 tccccttgga gaagcaatag ttaagtatgg agaagagaag aatataaaat ttgaaaaagt
     1861 tgataatttt aaagctatac caggtgctgg tatacaagtt actattaatg atgaaagtat
     1921 attacttgga aatagaaagc ttatgaatga caataatata aagcttgggg atttagaaga
     1981 aaaatcaaat atattggcaa gccaaggtaa aacacctatg tatatagctg tagatggaaa
     2041 tttatcaggt ataatagcag ttgcagatgt agtcaaagaa agtagtaaaa aagcaataga
     2101 aatacttcat gatatgggaa taaaagttgc aatggtaact ggagataatg cgaaaactgc
     2161 aaatgctata gcaaatcaag taggtataga tatggttttg gcagaggttt taccagaaga
     2221 taagtcaaaa gaagttgaaa aactacaaaa tcaaggaaag tttgttgcaa tggtagggga
     2281 tggaataaat gatgccccag ctcttgcaaa agcagatata gggatagcaa taggaagtgg
     2341 aacggatgta gccattgaat ctgcggatat agtacttatg aagagtgatt taattgatgt
     2401 tccaacagca attaaactta gtcatgaaac tataaaaaat ataaaacaaa acctattttg
     2461 ggcatttgga tataatacaa ttggtatacc tgtagcagca ggaatattat atgtatttgg
     2521 aggaccttta ttaaacccaa tgattgcagc agcagcaatg agcttaagtt cagtttcagt
     2581 cgtatcaaat gcacttagac ttaaaaactt caaggcatat aagagagatt agattaataa
     2641 attaaataaa atgtatccaa aagagctatc tttaaattaa aaggattaat ttacaaatta
     2701 agatagctct tttttcatgt aaaattcaca acaataagat aaaatataag taatatacga
     2761 aattttaaat aaaatagaaa ggattgatag aatgaattcg tcaatacttg taattgaaga
     2821 tgattcaaat atacaagagt taatatcaga atttttaagt gcagaaggtt atcaagttga
     2881 tacagcaaat gatgggttgg aaggtataca aaagtttaag caaggaagtt atgatttagt
     2941 aatattagat ataatgatgc caaacttaga tggttatgga gtttgtaaaa tgataagaaa
     3001 gtcatcaagc gttccaatta tatttttaac tgctttaaat gatgaaggag accaacttaa
     3061 agggtttgac ttagaatgtg atgattatat aacaaaacca ttttcattta atcttcttat
     3121 aaaaagagta gaagcaattt taaggcgaag taacaagact ataaatgaca aatttatagt
     3181 ttttgaaaaa ttaaaattag atttaaacac atatattgca gagatagatg gagaacctat
     3241 agaacttaca ctaaaagagt ttaatatatt aaaagctttg atagaaaagt atccacaagt
     3301 tataactagg gaaggtcttt tggatagtat atggggttat gactattatg gagatactag
     3361 gattgttgat gcacatataa aaaatataag aaaaaaaata tcattaccat atattaaaac
     3421 tgtaaaagga ataggatata cacttgagaa ggatatttga taagtgggaa aagttaagta
     3481 taaagtataa gttattttca ataacgacat cattattaat agcattagct ttaataatat
     3541 atctaatttt atatttttta ttgccatcat attatcatga gtataaaatt gagtctcttc
     3601 aagaaagttt gaaatcttta gtagatagtt caattcattt cgatacatat actttagaag
     3661 aaagacttta ttatatggct aaagaccaaa atttggctat tttattaaaa gataaccaag
     3721 gtaaaattgt atatggtaag aatgaagtag ttattttaag atatagtaag tacatgatta
     3781 atagtcttga ggatgagtat agaacttcta tacctatata tactaaagat gcaaaagatg
     3841 gtccatatac tttagaactc gttatgccat tacaaccaat tgacgaagca aacgaagtta
     3901 taagaaaact aatgccatac atcatatcaa tagcaatatt gatagcgatt attggagcat
     3961 atatatattc aattgtaata acaaagcctc ttataaacat aatagagagt gagagagaac
     4021 aggaatatag aaggaaggat tttgttgcta caatatcaca tgaactaaaa actccaataa
     4081 ccataataag tggtcaaatt gaaggtatga tttatagtgt aggtaagtat aaggataggg
     4141 atacttatct gaaaaaatcg tatgagtgta cacaagaatt aaaagattta gtaaatgaaa
     4201 tgatagaggt ttcaaaatca gaaatacttg aaaaagattt gaaattggta tcaataaata
     4261 taagtgaatt attaaataga cttgtaaaga gacaagtatt tttaatagaa gaaaaacata
     4321 tgaaaactat tttaaaaata gaagaaaatt tggaaattaa agcagaccaa gagagaataa
     4381 ctaaggcaat caataatata ataaataatg ccataaaata ttctcctgaa gaatcagagc
     4441 ttataattag actctatgat aaaaataaac gaataagtaa aaagacttct aatcaaagag
     4501 tggttttaga aattgaaaat actggtgtaa ctatcgagaa acgttattta gaagaaatat
     4561 ttaatccatt ttatagaata gaaaaatctc gttcgagaaa aactggagga agtggacttg
     4621 gactttatat agtcagccaa atttttaaaa gtcatgggtt tgattatagc ataaaaaaca
     4681 aagaaaactc tgtagtattc acagtagaat ttaaaaatta attacttatt ataaagaata
     4741 tataactttc aataatagtt cattttaaaa tcacaaaaaa ttcataatga aactgtatta
     4801 tataaatata aagaagctta aaaccataaa atacaccaac tataatgatg agatttcctt
     4861 acatcctcca tataagtcat cattatagta acccaacaat taagttacta tgaatttaaa
     4921 aaataacacc gagcatataa tctatgctta gtgttatttt tattttgaaa caaaaaatat
     4981 ttaatttatt gaaatgtata gatttagata gtttaaacta tatttaagac ttaaaaatta
     5041 taattgatat tacaaataaa tatatgtact ttgggaaaaa taaatatagg aagtattata
     5101 tttacagagg aggtagtttt gatggataga ttaaaaagag atatgaaaaa tatgaaagaa
     5161 gatttaaaag aaggtgttaa aaagacagct tatgttatga aaaatacagc tgaagatttg
     5221 aaagatgatg cacaaggtgc tatggtaaac ttagaaatga aaaaagatga gatgatggaa
     5281 agatatgaac aaaagaaatt ttctaaagaa ttagagaagc aaatgaagaa tgaaatgaaa
     5341 taagctttta tttatgaata acaaattatt gtattcatat aaaaagtagc cagtaatatt
     5401 aaatagttta tttaaggaaa tagactttct aaaaataact gtattagatt tagaaagtct
     5461 atttcaaatt agttttataa atagacataa ggataaagta gatgttatag aactagattt
     5521 taaa
//
LOCUS       DAEMIK010000204         5459 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEMIK010000204 DAEMIK010000000
VERSION     DAEMIK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.207"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..820
                     /locus_tag="KPK87_003539"
     CDS             <1..820
                     /locus_tag="KPK87_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2539643.1"
                     /translation="KFKSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGIVKVRDKQVP
                     KGRILNATISKEPSGRYYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIEN
                     PKYLKKSLNKLVKLQSELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLI
                     KENDIICIEDLQVKNMIKNHKLSRSISDVSWSEFIRQLEYKANWYGRQIVKVGKFFAS
                     SQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            947..2116
                     /locus_tag="KPK87_003540"
     CDS             947..2116
                     /locus_tag="KPK87_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG2539644.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(2279..2821)
                     /locus_tag="KPK87_003541"
     CDS             complement(2279..2821)
                     /locus_tag="KPK87_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG2539645.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2969..3253)
                     /locus_tag="KPK87_003542"
     CDS             complement(2969..3253)
                     /locus_tag="KPK87_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2539646.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(3316..4080)
                     /locus_tag="KPK87_003543"
     CDS             complement(3316..4080)
                     /locus_tag="KPK87_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG2539647.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(4362..4628)
                     /locus_tag="KPK87_003544"
     CDS             complement(4362..4628)
                     /locus_tag="KPK87_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2539648.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4670..5425)
                     /locus_tag="KPK87_003545"
     CDS             complement(4670..5425)
                     /locus_tag="KPK87_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539649.1"
                     /translation="MPHKLLRQYIAHYTISIPDTSIKENLTLIPDASGCMIFKFDKKG
                     IESAFWGATTKTIIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNT
                     LFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKN
                     ISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEIQNNKIPFATLAQDIGYYD
                     QSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1931 a          735 c          788 g         2005 t
ORIGIN      
        1 taaatttaaa tcaaagaaaa ttaatagatt ttcatataaa actaacttta caaatggaaa
       61 cattatgtat tgtggtcaac atataaaact acctaaactt ggtatagtta aggtaaggga
      121 taaacaagta cctaaaggga gaatacttaa tgctactata tctaaagagc caagtggtag
      181 atattatgta tcattatgct gtactgatgt agatattgaa gcatttgaaa atactaataa
      241 tcatataggt ttagatttag gaattaaaga attttgtatc tcaagttgtg gagaatttat
      301 agaaaatcct aagtatctta agaagtcatt aaacaaactt gttaaattac aaagcgaatt
      361 atcgagaaaa acaatcggta gtttaaatag aaataaagca agattaaaag ttgcaagact
      421 tcaagaacat atagctaatc aaagaaagga ttttctacaa aaactatcta ctaaattaat
      481 aaaagagaat gatattattt gcatagaaga tttacaagta aagaatatga ttaaaaatca
      541 taaactatct cgctcaattt ctgatgtgtc atggtcagaa tttattcgtc aattagaata
      601 caaggctaat tggtatggta gacaaattgt aaaggtaggt aaattctttg caagttcaca
      661 aatatgcaat aaatgtgggt ataaaaacga ggaagtgaag gacttaaata taagagaatg
      721 gatttgtcct agttgtaatg aaactcacga tagagatata aatgcaagta taaatatatt
      781 aaaagaagga ttaagactaa taacaattca aaataaataa cgtatgtaag aaccgtagga
      841 actacgggga tagcctgtgg agaattagta agacatattt tagtatgcaa aattctgtga
      901 agcaggaacc ctgtgacttt agtcatggga ggttcagggt agaaagataa atgaactttg
      961 taaagatgaa gttgtagagt tttgcaatag aataaaaggt aaagtggaaa gtgttgcaat
     1021 aagtggagta tttgctccag taaataaaca acaagaagaa gaagttgcaa aatgggttag
     1081 agaaatttta ggggatattc ctatatcttt atcaaatgaa ataggtaata taggtatatt
     1141 agaaagagaa aatggagcta ttttaaattc tgcattgtct caagtaggta aagctgtatc
     1201 tttaggattt gaaaaagcac ttaaagactt aggtatagat gctcaaatat atttcagtca
     1261 aaatgatgga acattaatga atttagagta tacaatgaaa tacccaattt ttacaattgg
     1321 atgtggaata acaaattcta taagaggagc ttccttctta tcaaaagtaa aaaatgcaat
     1381 agtattagat gttggtggaa ctacaagcga cttgggtgta ctatataatg gattccctag
     1441 agaatcatct attccagtta caattggagg agtacataca aactttagaa tgccagatgt
     1501 tttaactatt ggtcttgctg gtggtacatg tgtaaaaggc gaaaaagata atataaagat
     1561 aggtcctgaa agtgtgggat atagaataac tgaagatgca ataatatttg gtggagacac
     1621 tctaacttta tcagatgttg ttacaaaatt agatatggcg tttgaagata gaaaaagcaa
     1681 tgttgaaaac ttagataaag agtattgtaa tgaggtatat aagaaggtta ttttaaaatt
     1741 agaagatgct gtagaccaaa tgaagactag tcaagatgat gttgaattgg ttttaactgg
     1801 tggaggaagt ataattgttc ctgaaaaact aaaaggcata agcaatgtta taagacctcc
     1861 tcattatact gctgcaaatg ctattggtgc agctttagga caaataagtg gagatgtaga
     1921 aaaagtatac agtttagata aaatgtcgag agaagatgcc atagaagatg caaaaaaaga
     1981 ggctgtaaat aaagctctaa aagctggtgc taaagaagat actatagaag tattaaatat
     2041 agaagatgtt ccacttgcat accttcctgg taatgcactt cttataaaag taaaagttgt
     2101 tggtgattta atttaaagaa taaatataag tatttccaga attatataaa tatttctgga
     2161 ctcattttta aacttatttt atcaataaaa acagcataac ttttgactat attcttataa
     2221 gtttccgaac agccgatata tacctatttt ttgaggttta tcggctgttt gttatctttt
     2281 atcgcattcc aactattttt cttaccgtat ttgctgtacg aattgtaatc ttatcactta
     2341 catttgaact ggcagtcttt gaccaccaat ttgtcttttt ataatcttta cgactaaaag
     2401 accaaaagat agcattttta taatgatata ctttttcata ctcttcttta ggatttccaa
     2461 cctcatcata aaactcgtca taagataagt caggaaacat aaaaataaga ttatcatata
     2521 tttctttgtt gttatctccc caccaattag gagcattatt tagtatatct tttaactctt
     2581 cttgtgaaat aataaaaact ggtatttcca attcaaactg tttttttatc attaatctta
     2641 ttgtatttga gataaccttg ctgtcatcta catcacttaa aaagacaaca ttaccactat
     2701 taaggtatgt agaaacttct gaataaccaa gttctacaaa gcttgctttt aattcagaca
     2761 taattatctt gttcttacca cttatattga tacctcttaa taatgcgata taacttttca
     2821 tataataatt tcacctctaa acattcacat ttttctatgt agtacaaact ggaatttgca
     2881 tcttttataa ttctttcaaa acttcttcat atgtaatttt tttaatataa aaaaatcggt
     2941 gcttaatttg taccgatttt tgtttttctt agtaaagaac tcgttttttt gcttctgcta
     3001 tagctctagc agcacttttt gcttgcattc ttttttcttc ttctttttta aattcattta
     3061 ttgtttcatt tagattctta tcatgttcat ttttttcttt atcttcgttt tctctgtata
     3121 ctaatatata atctattatt gattcgttta ttatttgaga tatactttta ttttcattaa
     3181 ttcctattct cttaacttcc ttaagtagag tctcatcaat atgaaccgtt gtttgctttg
     3241 tattttttgc caccttaatc acctcttaaa aaaaattcta acattaaaat tttttttagt
     3301 caataaattt taatattaaa attctttaaa tgttttattt gattttctaa aattttctat
     3361 tgtatttatc atcttatttt ttatattttc acctagtaca gaagacttta gtctaggatt
     3421 ttcgatatgg tcataaattg gttctaaaaa ttcgacttcg acaggaacag agttaatttt
     3481 accaataggt tggtatcctt cataagtatc ttttgaattt tttattacta gtggaacaat
     3541 agggcatttt gctttatatg ctatctttaa agctccactt ctaaactccg aaaccaatga
     3601 atttggttct ttaatccatg ttaaatctcc ttctggaaac actgccatac tttgacctgt
     3661 aagtatattt tgagaggctt gggctatact ctttataccc tcacgattgt tttttctgtt
     3721 tacatataca catcttagta attttgccca ttctttaaat ataggtatat ttctccaaac
     3781 tggttcatct gcaattacac atccaattgg tctttctaca cttgctgtta gtatgaagct
     3841 gtcaagcata ctagaatgat ttacaacaaa tagaagtggt tcttttggca gtgtttcctt
     3901 accaattata ttaagctcta tgttcactat atctaaagat tttttagctt gtttttgcat
     3961 aaattcaaat ttttccttta agctaaattt atcaggattc tttttaattt taataagcct
     4021 aggtatagaa gatgtaaaac ctataagttg atatgtagca aattttaagt aattattcat
     4081 aaaattatct ccttaaaata aaattattta ttactgtaaa gattttaaca taaaataaaa
     4141 tttaagtcta ttttaattca attttgtaac atatatttta aatagacaga gattgagatt
     4201 ttatatttcc aattttagta atcagatttt atcaataaat tactattcta ttaatttaat
     4261 taaataacct ttaaatttaa ttataaaaac tgttgcagaa atttctacaa cagtttaaaa
     4321 aatttacctt acaaaaactt attttataac aagagagtat attataaggc tagtacagcc
     4381 cattttttgc actgagaaat tcttttgcca tcatcacctt gttctttgtc atatgcaaat
     4441 tgattaagca tttcacatgg aaatgtatca catagaccac aatgtgtaag gtttttatct
     4501 tcacaacaag attttacagg acattgttca ccccaaaatg gtttctcaat ctctgtacaa
     4561 cctttacaat tgactttttc cttgtatcca cattcataac ataaaattcc acatcttgat
     4621 tctatcattt taagtcactc cttaaatata aattttatat gattgtatat caaaatttat
     4681 acttttcatt gtaaaaatcg gacaaatttt ttatataggt tgttggatta acaccacaaa
     4741 tagatttaaa atcatttata aaatgagatt ggtcgtaata accaatatct tgtgctaaag
     4801 ttgcaaaggg tattttatta ttttgtattt cttgtaatac aaggtttata cgaagtaatc
     4861 taaggtatga tttaacactc ataccaagag aattattaaa aattctattt aaatgtcgtt
     4921 cactataaca actaatttgt gaaatgtttt taacagacat aatagagttc tgttttctag
     4981 cattttcaag aattggtata gtcatatcta ctatatttga tttcaataag tatgataaga
     5041 ataacatatc taattgttct actaattcct ttatagtact tgttctttca aaaatggaat
     5101 taacctctaa gctaaataat gtgttaaaat cttcaagtga aatttttaaa tctgtagatt
     5161 ctctttgaga aagacctgtc aaataatata ctccacctgg gcgaaactca acgaatactc
     5221 taaaaagtac attttctata tcatttttta caataatagt ttttgttgtg gctccccaga
     5281 atgcgctttc tatacctttt ttgtcaaatt taaatatcat acatccacta gcatcaggta
     5341 ttaatgtaag attttcttta atacttgtat ctggaataga tattgtatag tgtgctatat
     5401 actgtcttaa caatttatgt ggcaaaatat atgtatactg tttgcaggta tttaaaatg
//
LOCUS       DAEMIK010000205         5406 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEMIK010000205 DAEMIK010000000
VERSION     DAEMIK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.208"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(83..766)
                     /locus_tag="KPK87_003546"
     CDS             complement(83..766)
                     /locus_tag="KPK87_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG2539650.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(942..1532)
                     /gene="zapA"
                     /locus_tag="KPK87_003547"
     CDS             complement(942..1532)
                     /gene="zapA"
                     /locus_tag="KPK87_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG2539651.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1557..3950)
                     /locus_tag="KPK87_003548"
     CDS             complement(1557..3950)
                     /locus_tag="KPK87_003548"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG2539652.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(3967..4986)
                     /gene="pheS"
                     /locus_tag="KPK87_003549"
     CDS             complement(3967..4986)
                     /gene="pheS"
                     /locus_tag="KPK87_003549"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG2539653.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5036..5270)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1641 a         1061 c          595 g         2109 t
ORIGIN      
        1 ttctttaatt aatctataca atatttgcta tatataaagt atatcaaata acagcttcat
       61 ttttttgtaa atttacagaa agttaatttt cattaaatga atcatcttta ttgataaaca
      121 taattccaaa gaccactgcc aaaataaaaa aacatattag tattgatttt tccacgaaaa
      181 tttttgtaaa aaaagttcct aaaatagctc cagatataaa gaatataata atagcataat
      241 actgtaaact cttttttatc ataaaaatat tcttagtatg tatataatga aataaagttt
      301 ctgtagcact tcttaaattt ccagtacaca ttgtcgttgc aaaactattc ccatttactt
      361 ttctaaaact ctctacttgc attgaacaaa taaaagaaat agcaacattt acaaacatat
      421 taaatcttcc tctaggaata aatgaaacaa taagtattgt aatcatttca attataatga
      481 taacctgacg ccaatgaatc tttgtcttat tatttgtaaa atactttctt atcttctcgg
      541 ctataaatac acctaacata aatgccaaaa taggaatcaa ataataaaat gattccttta
      601 cattaccttc tgctatatta agcccaaata aaactatatt tccagtttgt gcattagcaa
      661 agactttacc tcttaccaca taagtataaa tatccaaaaa tcctcctgtt attgccaaca
      721 aagaacataa aaagaatgat tcagacatct gactcttgct tgtcatatat aaacctctcg
      781 ctttctaatg atagatacaa aatcaaaatc attttagatt ctatttttca ttgatataaa
      841 aaaattattt atatttaaat attgtctata tttatacatc atttatattt atacattaaa
      901 agaagctgtc tcaaaaaaca agagacagca aatcttttag tttattccac attttttgca
      961 tcattattta accctgtaca ttttaactgt aaatcataag ctttgttttg aaactcagaa
     1021 gctaagtttt cagctatagt agctctttca tttgcttctt caagttgagt acttaactcc
     1081 tcaatcttgt ttttataagc atctaactct gctctagaac cttctgtcgt attactaagt
     1141 tcgaatattt cttgcttttt attttctata atctcaatta actctttcat cttagtttca
     1201 gcttcacgat tttctgcttc tttttctgct atcgtaagct ttaaagattt gatttctaat
     1261 tgaagctctt cattagatgc tccaaccttc ttactcaatt cttcattcgc tttaatcaac
     1321 ttctcattct catctgaaca ttcaaataaa aggtctgata tatttaatgc agttaatatc
     1381 gcagcaactg ataagctcaa tttcgggttt tgacgtgtta ttttatccat ttctttgtct
     1441 acaaaatcag caatactaat cataaacttc tctgatttgt ctccaaccat tggatattca
     1501 gcgccatgga ttttaaccat tactttgttc atcctaacct cccccgacat tagttgttaa
     1561 tttgatctta agttagcatt taatttttcg cttaattcac ttaatatctt atcatgcacc
     1621 ttagctacat cttcatctgt tagagtttta tctttacttc tataagttat tgaatatgca
     1681 attgacttgt atccagcttc tatttgagaa cctttgtaaa catcaaataa cttgtagctt
     1741 tctactaatc cttgaccatt ttcctttatt atatcttcta tttgttttac aaatacatca
     1801 tctttaacaa taagagctat atctcttgat gtagatggat attttggtaa tggcacataa
     1861 ttctttgttc tatcagaatt atcaaataca aagtcaatat ctatttcagc gacataaact
     1921 ctttgaccta gattgtagtt ttctataaca tctggatgca attcacctaa tgtacctaca
     1981 tatttattat tatacactat tttagcacat ctaccagggt ggaaggtagt attattatct
     2041 tgaggttcaa tttcataacc tttaaatccg acatttacaa gtatagtttc tatagttcct
     2101 tttagtgcaa agaagtcaac atctttacca tacataccta cacacattct attttcttcc
     2161 ttaggtaatt ctgaatcttg tgggatgaat atatgtccac actcaaatgc agatacttct
     2221 tctattttat gagatacatt agttgataaa acatctaaca tatttggtat taaagtagtt
     2281 ctcattacag aagtttcttc tcctaatggg ttaagtaatt taacaaaatt tctctttgca
     2341 ttaccttctg gtactcttat cttatctact cccttagggc ttacaaatga atatgttaat
     2401 atttcattta atcctacagc agttgaatta tcttttaaag catccatgaa tttttgtttt
     2461 gaagttttaa ttcctgctgt cgtatttcct tctaattgta cagaaggtat attctcaaat
     2521 ccataaattc tagctatttc ttcccataca tcagcttctt gttccatatc tgttctaaag
     2581 cttggtacat ctatttctaa tttatcatta gctaccaaat tacatttaaa ctctaatgat
     2641 tctaaaattc ctacaaactg ctccattggt acatctacac ctaataggtg gttaattctt
     2701 tgaggattta ctaccaattt tttaggttct ggtttatttg gatatacatc tacaacacct
     2761 tttaaaactt ttcctgctcc taacatttca acaagttgtg cagctctatt tgctgctatc
     2821 tctgcaagat tagggtctaa gtctttttca tttcttgaag atgcttctga cctaatacca
     2881 acttttttag ctgtcattct tatgttttct ggtttgaaat tggcactttc aaaaagtaca
     2941 gtcttcgtat tagaagttat ttctgagtta gcaccaccca ttacaccagc taaaccaagt
     3001 gatttttctc catttgttat aactagcata tctttgtcta atgttctctc aacatcatct
     3061 aatgttacaa gtttctctcc atctttagca tttcttacta ctattcttcc agtctctact
     3121 tgatttatat caaaagcatg aagtggttga ccaagctcta acattacgaa gtttgttata
     3181 tcgactatgt tacttatagg tcttactcct gcttctgtaa gtcttctttg catccaatat
     3241 ggagaaggtt ctatttttac atctgtaacc attctagcaa catatcttct acataaatct
     3301 ggattatcta tctcaacttt gaaatctatc tcttcgtcac tttcttttac ttcgatttca
     3361 ggatatttta cttttgttcc tatagttgca gctgcttctc tagctatacc catcatgcat
     3421 ttacaatcag gtctgtttga agttaattca aaatctatta aagcatcttt taatcctaaa
     3481 acatctttta tatcttttcc taattcataa gaatcttcgt ggtctaaaat atatatacca
     3541 cctcttttat actcctcaat gtaatgttca tctataccta gttcagctgc tgaacacatc
     3601 ataccatctg ataattcacc tctgaaatca gtctgtttga tttcaactcc tccaggtaac
     3661 ttagaaccat ttacagctac tggaatataa tctccttctg atatatttgt agctcctgta
     3721 actatttgaa caactttatc tccaatatct actttagtta caaccaactt atcagcattt
     3781 ggatgttgtt ttatttctaa aatctttcca accaatatat tttctatttc ttcaccataa
     3841 taatctattg tttcaacttt agttcctgtc attgtcattt tatcagcgaa ctcttttaca
     3901 tccatgtcta tatcaacata gtctctaagc cattttaaag atactaacat ctacataccc
     3961 tcctaattaa aattgattta agaatctcat atcattttcg aataataatc taatatcatc
     4021 tatttcatat ttaagcattg caagtctttc aaccccaact ccaaatgcaa atccactgta
     4081 aacttctggg tctattccac aatttctaag cacatttgga tgaaccatac ctgaacctaa
     4141 tatttctatc caaccttcat atttacacat tgggcaacct ttaccaccac atttgaaaca
     4201 agtaacatca acctctgcac ttggttctgt aaatgggaag ttgtgaggtc taaacttagt
     4261 tttgatatca gaaccaaaca atttttcaac gaagatatcc atagttcctt taaattctgc
     4321 catagtaaca tcttttccaa caacaagccc ttctatctga tggaacattg gtgagtgtgt
     4381 agcatctggc gagtctgacc taaaacatct acctggtgca attactttta ttggtaactc
     4441 ttgacttctc atagttctta cttgaactgg agatgtttga gttctaagta atactccatc
     4501 attgatatag aaggtatcgc tcatatctct tgatggatgg tctttaggag cgtttaatgc
     4561 gtcaaagttg ttttcaacag tctcaacttc tggcccttct gctatagaga atcccattcc
     4621 gataaatata tctgttactt catctataat ttgagttatt ggatgcttct tcccaacctt
     4681 taaaactttt cctggttgag taacatctat cacttcctct tttaattttc tttctttttc
     4741 aatagcattt atttcttctt ttttagaatt tatgctaagt tcaatgtttt ctcttacctc
     4801 attggcaacc ttaccaacta ctggtctttc ttcagcagat aatttaccca tttctttaag
     4861 tatggcagtt atctcacctt tttttcctaa gtacttaact cttaaacttt ctacactttc
     4921 tatgctttgt gcttctttta tttcagccaa agctgcttca cgtaaagcaa gtaatttttc
     4981 ttgcacacat atcacccttt cgtttttttg tataaaaaaa gcccctcaat ccctgtctag
     5041 ggacgaaagg ctatagtttc gcggtaccac cctagtagtt taacaattta aaaatcatca
     5101 aaccacctct aacaaactta acgcgttttt gacgtcaaag cctactgtaa aatttcagct
     5161 ctgttactcc agagggaact tctcatgacc tcttagaaaa cactcccagc ctaggtgttt
     5221 atctctttac taagtttaat caaaatactt tcctctttca ttgcatttac aatattatat
     5281 tgtttatttt agtattatat catttccatt caaattatgt gactattttt aatatatatc
     5341 caatatataa ttaatttaat tattttttat ataatatata tctaatgcat aattaactca
     5401 attatt
//
LOCUS       DAEMIK010000206         5316 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEMIK010000206 DAEMIK010000000
VERSION     DAEMIK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.209"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(413..1819)
                     /locus_tag="KPK87_003550"
     CDS             complement(413..1819)
                     /locus_tag="KPK87_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2539654.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSKNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1864..2571)
                     /locus_tag="KPK87_003551"
     CDS             complement(1864..2571)
                     /locus_tag="KPK87_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539655.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2742..3656
                     /locus_tag="KPK87_003552"
     CDS             2742..3656
                     /locus_tag="KPK87_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539656.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3656..4417
                     /locus_tag="KPK87_003553"
     CDS             3656..4417
                     /locus_tag="KPK87_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539657.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4429..5166
                     /locus_tag="KPK87_003554"
     CDS             4429..5166
                     /locus_tag="KPK87_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539658.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1828 a          819 c          697 g         1972 t
ORIGIN      
        1 tacatagaag ctattataac tacacagaac agcgtataat taaaactttt ttaggatgtc
       61 attatatttt ttcttaaaat taaaaaacac tataccataa ttctatttta gtagaatttt
      121 tttgtcaata ataaaattaa atattaacta aaaaataaaa aaaatggaat tatctaaaga
      181 atttaataac cttacaataa ttccaaaata ttatttctat gaaaattttg ataccatgag
      241 taactcagcc ataagcgcat aaagcacaag ttactcttta ctttaataaa acttgacaag
      301 aaaagaattt atccttattc tactctaata gagttaattt gtcaataaag aataaaatta
      361 ttttcaaaat attttcattc ttcattctta tctgttatta ttttataaaa tatcacttaa
      421 ttagaggtaa ttttagtatt gtttttagtc cctgactatc aatgctcgta atatctaatt
      481 tacctctatg tgcattaata atgccagata ctatcaaaat acctagacca tgattttgac
      541 ctttggcata tgcctcactt agtccatttt tgctctttaa taaactatta attctttctt
      601 gactcatacc ttttccatca tcttcaacaa ttatatagca aaaatctaaa tcagacttag
      661 aggtaaccct tatattacat ccttgttggt tatgagttat actattttgt attaaattac
      721 taatagccct ttttaataat tttttatctg catctataat aatatcttca tttagtatat
      781 caagttcaac gtaaaagtca ttatccaaat tattatttat aaactcagaa ataagctctc
      841 tcaaaaacgc agaaactcta atcttatcac aattcaatgt ttgcatatta tattcaagtt
      901 tagacactaa attgaggtca tttactaatt ctctgatttt aatcccttga ttgcatataa
      961 tagacacttg ctcttgttct ctatttgaaa ggctactgct ttcttcaagt tctccagcat
     1021 atccaattat cattgaaagt ggagttctta tatcatgaga tattcctgca atccagttag
     1081 acctagcttc ctctttcttc ctaagttttt catctttcat ttgcaactct ttagatacag
     1141 tattaataga ttttgaaata tcacttagta ttcctttttc ttgtaaaagt actggttctc
     1201 cttttgacat aagctttatg ccagtaataa ttggttttat agatttcacc atttttaaac
     1261 ctatgaatag agctattaaa aaagtaacta ttgcattaac cagaaacata cctattatta
     1321 acataggaaa ttgttctatt tccttaaccg tataagtaag gttaaactta gtgtacttgt
     1381 cttttggata acctgttact aatattccat ttggatgttc ccatacatac acaggatagt
     1441 cctttaaaaa gaaccttgaa aactttgcta catctgtcaa agtataatga cttggaattt
     1501 cttttggtaa atcaaatccc catattctat ctccattgtt atcaattaac attgcccaaa
     1561 ctttattttt atgtagtact tccttactag aatcatctaa aataaacttt ccatttctta
     1621 aaatcaaatt atcttcaatc ataatcaagt ttgattttat ttctctgcta tcacttgtat
     1681 ctaaattatc agagtattta gatacaaata gcatatatga aaacagaaga aaacctgcta
     1741 tatttaatac aataaaaagt aatgaaatca agatacttga acctataaat cgcctcaata
     1801 tgtactttac actatccaaa attaaatcac tcctcttgat ttcttttatt ctaatatcta
     1861 aaattaatta atcatcaact tatatccaag acctcttact gtaataagat gtttaggtgt
     1921 agatggagtt ttttctatct tctctctaac ccttctaaca tgaaccataa gggtattttc
     1981 atatccatac atatcatctc cccaaatggc ttgacatagt gaatcattag taacaattct
     2041 ccctttattt tcatacaact ttaaaagcaa cgaatgctct ttagctgtta aagaaatctc
     2101 ttgcccatct ttctcaacac atgcactatt taaattcaca acaacagaat ctcccagttt
     2161 aaatactggc aactcctctt cttctatagg cacatataca cgatttaata tacccacaag
     2221 ccttaaagta agttcctttg gtaaaaatgg ctttactata taatcgtcag cccctaagcc
     2281 caaaccaatc aatctatctt catcttcacc tcttgcagag agaaaaatca ctggtatttt
     2341 agatgtctct ctaatttttc tcataataga aaaaccatct ccatctggca atacaacatc
     2401 taaaattgca caatctggct ttacatttct aaatacccta acagcttcct cataacttga
     2461 tgctaaaaat actctactaa atccctcttt tctcaggatt tcatctatca tttttaataa
     2521 ttccttgtgg tcatcaacca caagtatttt tttatctttt attgactcca taatcatcac
     2581 cctcctttag tataccatat acccattttc acaactttgc ctaaatagac ttttaaggtt
     2641 attgtaaggt aaatttcagc taattgaaat gtaaccaagt tattatactg atataagtta
     2701 aaagaatcaa taagcacaga ttaaaaaagg ggatgaaaca tatggaaatg ataattagaa
     2761 cacaaagact ttcaaaggaa tacaataaaa tatttagagt aaaggatata gacctaagag
     2821 ttccacaagg agctgtatat ggttttttgg gtcctaatgg agctggaaaa tctaccactc
     2881 ttaaaatgct tcttggactt gctaaaccca cacaaggaaa tattaatatt ttaggtaaag
     2941 agttaaatga aaaaaataga atatcaattt taaaagatat aggttctttg atagaatctc
     3001 catcatacta tggtcatctt acagggcttg aaaacatgac cataatgcaa agactcttag
     3061 atttacctaa aaaaaatgta aatgaagcat taaaaattgt aaggttagaa aatcaaaaaa
     3121 ataaaaaagt tagtcaatat tctttaggta tgaagcaacg tcttggcatt gctatggcaa
     3181 taatgaagtt tcccaaatta ttaatacttg atgaacctac aaatggactc gacccagcag
     3241 ggattgagga aattagagaa ttaataaaat ctctaccaga aaaatacggg atgactgttc
     3301 taatctctag ccatctttta agtgaaattg accaaattgc cacatcaata ggaataataa
     3361 atcatggcga actggttttt caagatagca taaaagaact tcataataga ggtcaaagtc
     3421 agattgctat caaaactaag aatgttgtat ctgcacaaaa actattaaat actaaaggat
     3481 atatgtcact tatacaagat gggtacttga ctattgaaaa tttaaaagac tcagatgtgg
     3541 ctaaaataaa tggtatgctt gtccattcaa atatagatgt atttagaata gaagaacata
     3601 aaaaaagcct ggaagatata ttcttagaac ttactggtaa ggcggtgact ttataatgaa
     3661 ataccttggt ctagaatttt ataaacttaa aagaaagaag attattctga tgaccttttt
     3721 atttgtagct gttgaaataa tgtggtgtat cgtaaattca aatagggcta ttacacgtaa
     3781 tccagatatg ttaggtggat ttgaatattc ctatatgctt atgagttttg caagtttaaa
     3841 tggtctattc tttcctatct taatatcaat aattacatca cgtataagtg atatagaaca
     3901 taaaggagat acatggaagc ttttaaaatc ttctgtgaca tctttaaata gtatttattt
     3961 atctaaattt ttatgttctg ctattttggt atctatacct gtattgatgc aagtgttatc
     4021 tattgtgttg tttggacatt ttagaggggt tattgagtca atttctgtat ctttactttt
     4081 aaaatacaca ttaggaacaa ttcttgtaag tattgctatc attgcactac acctatggat
     4141 tgcaactgta attccaaatc aaatgattgc aattacattt ggcatgattg gtagttttat
     4201 aggtttgaca tcaggtttgt tttatcaagg tattcgtaga ttgtttatat ggtcttatta
     4261 cctagagtta tcacctttaa gttatgctta tgataatgtt ttaggttcat ctgtctatag
     4321 aattgatatg aatttttcta ttacgatttt agttttctta attggaatgc taatttatta
     4381 tattggaaga aatcatttat taaaaaaaga agtataggag ggatttaaat gttaaggaag
     4441 tcagtcttgg gagaaattct aaagttaaaa aattctttta tatggtatgt attattagct
     4501 ttgccactta taagtgtgtt gattggttct ggtaattttt atctaaatca aggcatactc
     4561 aaaaaagaat ggtacagctt atggactcaa gttagtcttt tttatgggga atttttcttt
     4621 cctatcttaa tcgcaatctt ttgtgcatat atttgtaggc ttgaacatat gaatcataat
     4681 tggaataatg taatgacact gccagttaat ttaaaaaata tctttttatc taaacttaca
     4741 gtagtttcaa tacttacagc tattacacaa gttttctttg ttatctttta tatagttgca
     4801 ggaaatatgt ttggtttttc tggtagtgta cctaaagaat tgactggttg gattgctaaa
     4861 ggttggatta tttctatttc aatagcttct atacaattat acatgtctat aaagattaga
     4921 agttttgcaa ctcctattgg tattagtctt tgcctttctc tatttggtat gggattaata
     4981 gtagcaaaac ctgctgttgg tatatttttc ccttattcta tgttaggtag tgctatgggg
     5041 gttataaagc aatctgcttt aaatgttaat gacactatag ttacaatatg tgttgcattt
     5101 attactacta tagtattttc tgaaattgcc aatagaaatt ttagaaagaa tgatgttatt
     5161 tcttaatggt aaatgctaga tatttttggg tttgaaattt atattctttt tagttgaaat
     5221 ctatatccat tttatttaaa aatatatatc ttttatttga attttatatg ttttatctac
     5281 tcgctatata atagatatac tcctaaaatt aaaaaa
//
LOCUS       DAEMIK010000207         5205 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEMIK010000207 DAEMIK010000000
VERSION     DAEMIK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.210"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            872..1633
                     /locus_tag="KPK87_003555"
     CDS             872..1633
                     /locus_tag="KPK87_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2539659.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEKCIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1698..2480
                     /locus_tag="KPK87_003556"
     CDS             1698..2480
                     /locus_tag="KPK87_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG2539660.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKKIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            2747..3166
                     /locus_tag="KPK87_003557"
     CDS             2747..3166
                     /locus_tag="KPK87_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG2539661.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(3269..3535)
                     /locus_tag="KPK87_003558"
     CDS             complement(3269..3535)
                     /locus_tag="KPK87_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG2539662.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3762..4463
                     /locus_tag="KPK87_003559"
     CDS             3762..4463
                     /locus_tag="KPK87_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539663.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4545..5153
                     /locus_tag="KPK87_003560"
     CDS             4545..5153
                     /locus_tag="KPK87_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2539664.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
BASE COUNT         2150 a          476 c          903 g         1676 t
ORIGIN      
        1 atttttataa acaagtaatg ttattaaaat taagtatata gttaaaagtg tatagcaaaa
       61 aggctgttat attaatgtta gaggttcagg gaaaatatat aaagcaaagt atttacagat
      121 aatgtagatt gcacagctat aagttagata atagaattat gtaatcaaga atttacaaca
      181 aagtataaga taaggataat gctaaattgc catacaggtg ctagatgtgt aaatggattt
      241 aattaatact attgataaag tgcaacaagc acataaacca attgatgaga ttattaaaac
      301 tataagagat atagttgaca taattaaaat attggaacct atttataatt ttaaatcaaa
      361 tcaataatga ttaagattaa aataatatta aggcatcaat gttataagag tacaatataa
      421 ggagtactat atactactcc ttacttatac atattaggca ataggaaatt ttttcatagt
      481 taaattttca aatatttttt agggaacttt ataattgaga attaagggta tagtatgtga
      541 tgaacttact aaatttataa ttaggttata taatggaata atcatcaata gttttacttt
      601 agggaaaata tatgcttaag gaagtatagc ttactaatat tattaaaatg tatatttaaa
      661 atgataaaaa aagaattaga caagtattta gtaatttgat tatgcaaaat tctatcaatc
      721 aggaacccta tgactttagt tatgggaggt tcagtaggta acttgttgaa tgaagtttat
      781 attatttaaa taaactactt aattcagata atataaaaag tcagaatttt aagaatagag
      841 ctatataatc ttagctatta ggagaattac tatgatagga atatatttta gtggaacagg
      901 aaattcaaga tactgtgtag aaaaattttt acaagaatat gatataacag caaattctta
      961 ttctattgaa gataatgaac ttttacagca catcaataat catgagaata ttatttttag
     1021 ttatccggtg caatatagta atgtgccaaa aatattgaaa gattttataa taaatcattc
     1081 aactttgtgg aatggtaaac gtgtttttat tattgcaact atggctctat ttagtggaga
     1141 tggctctggt gtgttagcta gaatattaaa aaaatacggt gctataactg ttggtggatt
     1201 acatgtgaag atgccagata gtataggaga tgaaaaggta ttaaaacatt ctttagagta
     1261 taatagaaag cttgtaataa aagcagaaaa gaaagtaact aaggctgcta aaaaactgaa
     1321 acatgggtca ccaccaaaag aagggttggg ttttttgagt catattgtag ggttatttgg
     1381 acaacgattg tattttatca ataaaacaaa tatgtataca gataaactaa aaattaatac
     1441 tgagaaatgt attgtatgtg gtaaatgtgt taatttatgt ccaatgaaga atctagtaat
     1501 taaaaattgt atgattgttg caaatgggca atgtacaatg tgttatcgtt gtgtaaataa
     1561 ttgtgccaaa aaagcaatta cattgttagg taaaaaagtt gttgaacaga gtaatattaa
     1621 aaaatatatg taagaaattt aaagttttat tgaatgtaaa ttgatgtata ttttatgtta
     1681 gaaagagagg ttaaataatg gaatactaca tagttgattc atttgcaact aaactattta
     1741 aaggaaatcc tgctggagtt tgtgttcttg acagaagaat acctttagag ttaatgcaga
     1801 aaatagcaga agaaaacaat ttaccagaaa cagcatttgt agttaaaggt aaaggaaatt
     1861 atgaattacg atggtttact cctaaagcag aaattgattt atgtggtcat gcaactttag
     1921 cagcagctta tgtaatatca aattttattg atgttaatgt aaaaaagata gatttcttta
     1981 ctcaaagtgg aaagcttgaa gttacacgaa atggaaatct atatgaaatg atatttccag
     2041 aaataatgcc tattgaaata gagttatcac cgcaacaagc taatttgata ggctgtgtac
     2101 caagtgctgt ttattcttca agagatttaa tcttattgct aaacagtgaa caagaagtta
     2161 taaattataa acctaattac gcacaattgc gtaaattgac agactggtta ggtataataa
     2221 tcacagcaca aggaagtaat acagattttg tatcaagata tttttgtcca gaattagatt
     2281 cagaagaccc tgttactggt tcatcacatt gtaatcttat tccatactgg tctgaaaagt
     2341 tgggaaaaca caaaatggta gctgctcaac tttctaatcg tggtggtata attcagtgtg
     2401 aagtgttgaa ggataatact gtgaaaattt ctggtgaagc agtcttgttt atgcagggaa
     2461 caataaagat tgatatttaa ataagtataa taactaattt attatgaaaa ataattaatt
     2521 ttcttagtgt taacaataat aaagtagagt taaaaaactt gatttcactt aaatcaatat
     2581 gatagatgtt gatactaaga ttgacaagca tgattaaaaa agataatatt taatctataa
     2641 agataaaaac acagtctggt tggtagtcca gacgcagcaa tggctgtcag taacctgcct
     2701 ccttggttgt ccgttcttta gaaagtaatt taaggaggaa ctagcaatgg acaaaattag
     2761 tggaaaatta acagtcttat ttgaagaacc attttgggta ggtatatttg aaagacaaga
     2821 tggtaaaaaa tatgaagcat gtagggtagt ttttggagca gaacctaaag aagtagaagt
     2881 gtatgaattt atacttgaaa gatttttctc attagatttt ggtagcatta aattagaaaa
     2941 aaatgtgacc aaagacaaca ttggatacaa aagaatgcag agaaaagtaa aaaaggaaca
     3001 agaaaaagaa actataggga ctaaggctca aaatgctctt aagcttcaat atgaagaaag
     3061 aaaacaggat agaaaaaacc tagctaaaaa tagaaaagaa gaagaaaaag aaagactatt
     3121 taatttgaaa caagaaaaga gaaaagctaa acataagggt cattaatttt aattaaatat
     3181 ctttttaata attgctagaa aaaggtgtat aaaattttta attttataca ccttaattta
     3241 ttgaaaagaa taaggtttta tatttcaatt actttaaaat ataaaaatat actaataaaa
     3301 attgtaaaaa tagtataaat ggtataccaa atgtaaattt aatgtgttta gttttatgtc
     3361 taaaagaata cattccaatt atagaaccta tactacctcc aattactgct atagacataa
     3421 gagtagcttc ttttattctc cattcgtttc taattgctct tttcttatct atgtacattg
     3481 aaaagaaagc aatagaatta atcactataa aatatattag aataaaattt ttcacagtct
     3541 tgcctccata aaaatatatt atacattata aaacaagata tttgttttgg aaagttaaat
     3601 ttgtaattag tacatataaa atatttgata agaataaata taatattaat tggaaaattt
     3661 aaaaaaatta aacaattagc aaataggggg tatatttata agacacaaag tgataaaatc
     3721 tatttagaaa ataatgtaaa ataacaggag gataaagcaa aatgaaattt atagtagaaa
     3781 aagaagtttt tgataaatta gaaaatgtat gctttggtgt agtagttgca aaaggaatag
     3841 ataatactaa agaaatagaa agaataagca accttttaga tataagtata gatagagtag
     3901 aagattactt caaagataaa aaagtaaagg aatcagaaga aattatacca tatagagagg
     3961 catttagaag tctaggcatg aacccaaata aatttatgag ttcaatagaa gcaatgacaa
     4021 caagggttgc taagaataag aaacttcctc atataaatcc tattgtagat ttaggaaact
     4081 ctatttcttt aaaatattta cttccaatgg gtgctcatga tatggatttt agaaatgatg
     4141 atgtatatgt tagattttct aaaaaagggg ataaatttgt accatttgga gaaactgatg
     4201 ttgaattaat ggaagaagga gaattaattt attcagttgg agacatggta aaaactagaa
     4261 gatggatatg gagacaagga gaggaaggaa aaattactaa tagctcaaaa aatatatttt
     4321 ttcctataga tggttttact gatgctaatt tagataaggt aatgagtgca agagaagaat
     4381 tagcaaaatt gttaaaggaa atatttaatt gtgaaattaa agtaggtttt gtagataaag
     4441 ataatcctga aatggaaata taataagttg ttgaatactt gttaagatat tatatttact
     4501 taatgatgtt aaaaaggtat taaaaaagaa aggtcgtagt aaatttgaaa aaaactataa
     4561 taaagaatgt aaatccaatg attgaaaata agtttatagg actttttgaa atagagtata
     4621 aaaacaaatt agatgaagat aaggtttgga tggtagcatc aagaaaaagt agtgagcaat
     4681 taaaaaatat ttatttgaag aatgaagaag atagtgtaga tgcagttgta atagttgggc
     4741 ttcataaaag tagtaaaaaa ttaattttaa taagacaatt tagagtacct attaatggat
     4801 atatatacga gttaccagca ggtcttgttg atgaaggaga gtctatagat atttctgttg
     4861 aaagagaact tagagaagaa acaggactta ctcttttaga aattaaaaaa aataaaagta
     4921 ttgataaagt ttatttatct cctggaatga gtgatgaatc tgttgccttt gtttattgta
     4981 tatgtgatgg tgatataaca gatgaatttt tagaaccaga tgaagagatt gaagcgcttt
     5041 taatttcgca agaagatgca aaagagattt tacaaagtaa tcataagata gacacaaaag
     5101 catttttgat tttacaaatg tttgtgagtt taggagctaa attatttgaa tagatatata
     5161 gaaaagaggt tatatagaag agtaaaataa taaagtttat tttac
//
LOCUS       DAEMIK010000208         5136 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEMIK010000208 DAEMIK010000000
VERSION     DAEMIK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.211"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            245..736
                     /locus_tag="KPK87_003561"
     CDS             245..736
                     /locus_tag="KPK87_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG2539665.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDREFPVMLITANDVKELSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
     gene            1265..2137
                     /locus_tag="KPK87_003562"
     CDS             1265..2137
                     /locus_tag="KPK87_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539666.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            2134..3423
                     /locus_tag="KPK87_003563"
     CDS             2134..3423
                     /locus_tag="KPK87_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539667.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVISNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKIISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            3353..3997
                     /locus_tag="KPK87_003564"
     CDS             3353..3997
                     /locus_tag="KPK87_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539668.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(4264..4728)
                     /locus_tag="KPK87_003565"
     CDS             complement(4264..4728)
                     /locus_tag="KPK87_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539669.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLRDGEFFVMRYQ"
BASE COUNT         1967 a          571 c          755 g         1843 t
ORIGIN      
        1 taatttctta aatttttttt aaaagtaata gttcttattt atagtcaata ttgcctatag
       61 aacttctgat tcatttaatt tttaggaaaa aataataata cttacgctat aaacacagaa
      121 ttttagttga taatataggt atttaaattt aagatgaaat aatacttttc tcaatatatt
      181 ttattgtaac taagtttaat tataagttac aataaaaaaa acgctactta ggaggaatgt
      241 aattatgggg atgataggat gttatactaa aataagtgaa gagaatgttt ttaaattaca
      301 gcaagcagaa gaaaacttac aagattttgt ttttgaagat gccaacgaaa atagtactat
      361 caatattgat aaagcatggc atgccattca ctttactttg acaggttgtc cttttggtgg
      421 agatgatgat aatatattta gcaaattagt actaagtgga aacatactta tggaaattga
      481 tagagaattt cctgtcatgt tgattacagc aaatgatgta aaagaactat caaaagcaat
      541 gaacagtttg gaagaacaag catttagaaa aagatttaat attaatgaga tgttagaaaa
      601 taatatttat cctgttatga atgatgagag tgaagatgat ttttttgaat atgtatgggc
      661 aaatctaatt gagcttaaaa agtttataca agaagcatct gatgatggac tagctgtaat
      721 attttttata atgtgataat tactaaaaat tgtgaatatt agtgtcatca catatcttag
      781 atatgaagcc ttttgattgt atagaagaaa atacatatta tattagcctt ttaaaatata
      841 taagttaaat ttaaatctgt aggtaaattt gtcttatgtc tttataaata tttatattag
      901 catatataaa ttagttttaa atttttgcaa tatatagttg cagatatttt tatttatctt
      961 aaaaggctaa ataccctatc ttattatatc cagtaattct tttggatact tatcacattt
     1021 ttcattatat ataaaaatag actcacaaat aaaataaata tatattaaat tataacttca
     1081 taaacttaaa aaatttcaga aacaaagata ctttaattta attaaaaaca gaaaaattaa
     1141 aatctttaga aaaaatttag aaatttataa ataatatctt aagttttaga atttattatg
     1201 aaaatacagt aaaaatttat aaaaataaaa ttctataaaa ttatttctgg gggtgtattt
     1261 atatatggaa atgaaaatag cagtatccaa tcttagtaaa agatttggga aaaaacaagc
     1321 tcttgataat ataaatcttg aaatcgacag tggtatgttt ggtcttttag gaagaaatgg
     1381 agcaggaaaa accacattta tgagaatttt agccacttta ttatcaaaaa cagaaggttc
     1441 aatacatatg tgtggagttc ctattgagaa tactaaggaa attagaaata tggttggtta
     1501 tttgccacaa gatttttcaa tgtatcctaa catgacagta tataaagcaa tggattattt
     1561 agcagtttta tcggatatac cactaagaaa aagaagaaaa attatagcaa agttattaag
     1621 caaagtaaac cttactaatt atcaaaatgt gaaagtaaaa tctttatctg gaggtatgaa
     1681 tagaaggctt gggatagcac aagcattaat tcataatccc agagttttaa tagtagatga
     1741 gccgacagca ggtcttgacc cagaagaacg tataagattt agaaatttac tatgcgaaat
     1801 tgcaaatgat agaatcgtta ttttatctac acatattgtt ggagatatag aagctacatg
     1861 tgaaaacgta gctatattga atagtggaaa agtcatatac aatggctcaa cagaagcatt
     1921 agcaaaacta gctgaaggga aagtatatag tattgaagta gataaaaaag acattgaaaa
     1981 tataaaaagt agatttatag ttataggaat gttgactcat ggtggaaagg ctatacttag
     2041 gattatatca gatgataaac cttttgagac agcagtaaat tgtaatccaa ctattgaaga
     2101 tggctatatg cttataatgg gaggagataa tatatgaatc tatttataaa agaatgtaaa
     2161 aatatattaa agagtatggt atattatatt tttattgtag ttttagtttt tttctatatt
     2221 tctcaacttg gaagttcagt ttcaaatgat gtaagtaaaa atattcctaa tgatggaaat
     2281 ccattagttg ctccacaaaa agtagagaat attgaggaat attactctgt acacaatcaa
     2341 tatccatatg gtcaaaaaga atctatgaaa acagaaaaag tcatttcaaa tgcaactata
     2401 aacctaatta gagaatatca aaaaaacaca tatacaacgt atccaacagg tgtagttaag
     2461 tatataaaat tagaccaaaa taaaacgaag accatagaaa aatatataga agaaataact
     2521 ggtaaaatta ttagcgaaat aaattcactt tggaaaactg atgaaagtat aaaaatagat
     2581 tattctaaag tatctcttga aagatttgaa gaaataatgc aaagagtaga cgacatttta
     2641 ggaggagggt ctgcatatgc ttttgacaag ttacaaagat ttggagtagt tccagttacc
     2701 tatgatgaag ctatgaaaaa ttacaactat acaatacaaa aagataaagt tagtaatggg
     2761 tatgcaagaa ttttttgcga ttatataggt atagttttaa gtatatttcc agtatttgtg
     2821 gcagttttta tgtcacttaa agataagcgt tcaagaatga gagagattat ttattcaaaa
     2881 caagtatctt catgcaaaat agttttatca agatattttg cacttgtctt tatgatgata
     2941 ttgcctgtat taattttagg tatgaaagat ttacttcctt tgattttctt tgctaaagat
     3001 aaaggtatat ctattgataa cttagcattt ataaaatata cattatggtg gatactacca
     3061 actcttatga tagttacagc tgtaggaatg agtattacta tattaacaga tacaccagta
     3121 gcaattattg tgcaattaat ttggtggctt tacagtataa attctacagg actcaaaggt
     3181 gattattcaa caatgggact aatgataaga cataatagtg tattaaatgg ggatattatt
     3241 atacaaaact tcaatcaaat tatgattaat agaattgtga taagcttggg agcattggtg
     3301 cttgttttgt tagctatatt cttatatgag caaaaaagaa gggggaatct tgatgtttgg
     3361 gcaaagtttt caaaactgtt tagttctaat aaaagaaaac ttcaaattaa gcactttaac
     3421 taatttaata tgttcaatta tattgctatg tatgattcct ctaatacaag gagtagctaa
     3481 tttagacaga atttcaacag ctatatgttt agaaaattat gttgcaataa tagggatagt
     3541 aatgttggta cccgtatttt cgctagagca atcaaaagaa attgatgaga tagtatcttc
     3601 aaaagcatta tctcaagtta ttgtacaatt aattaggata ctgatgtcaa ttataggtat
     3661 agtggtatta atcataggtt ttgtaaatgt tttaatatat cttggatgta attttccagt
     3721 agttgagtat attattggaa gtttcgcatc agctatgttt ataggaagtt taggaatgtt
     3781 tttctcaaat ttatttaata atacaatcat aggttatatg atatctatag gatatttaat
     3841 tttaaatatg atgactaaag gcaagtatgt tggaaaattt tttataatgt ctatgagtca
     3901 aggtagtttt gatgagaaat attggctgat acttggaagt gttgtgttga taattgcatc
     3961 tctcttaata aaaccagtaa aaaggaagat tagataataa aatgagagct tagataaaat
     4021 aaaggtaata caaataaaaa gtacatttat gaaatataaa tctattttta tatacacaaa
     4081 cctacttaca atctctttat atatgtagat agatttatat atccgtagta taaaatatat
     4141 atgtatacaa aattttatca aaagctctca aatttatatt tcaaagacac ttattgctat
     4201 ggcctacaca tatgtatttt tttattttaa ttgttgtatt aacaataaga ctattgttat
     4261 aaactattga tacctcataa caaaaaattc tccatctctt aaatgcgttt tttgtacctt
     4321 agtctcaact attttaaatc cttgagactc ataacatttt acagctctca tgttccaggt
     4381 tcttacattt aatacaattg gtttagtact atatttattt ctacattctt gtaatcctag
     4441 gctaattatt tctttaccaa ttcccattcc acaataattc ggtttcatac caataccaaa
     4501 gaatacagag cttccttcat ctaataagtt tataaaacct atcaattctt tattgtcatt
     4561 tataaaactt ataaaatttt ttcttttatc ttcttttgct agtgaaaaat tcttcttttt
     4621 aatttcctcc catggaggta gattgtatat tgaatattta ccttcatatt tccaattagt
     4681 aacttcaata gcatgttctt cagtaaattt aactttattt aaattcatat gtcctcctat
     4741 taaaatattt aaggtaatta atggtatttt aaagttttag aaaataagta aaactattta
     4801 tatttatcca taaaatcttt aaaaatttta ctaacaattc ttgtaccctg tactttttat
     4861 tggattcaca gaagactata aactaatttt gaatcacctt ctaattcaaa aacatcacga
     4921 tatatataat ttcttagctt gattaaattc tgttaaatcc gtttcttata gttgtataat
     4981 caacatcaat atctttatgt gtattagtta atttattggt tttaggaact tattattaat
     5041 atattgtatt tgctccaata atccaagtag taggtaaatt ataggcaaat tatcaaatgt
     5101 atatctatcc atgcttaaac tttagagata caataa
//
LOCUS       DAEMIK010000209         5130 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEMIK010000209 DAEMIK010000000
VERSION     DAEMIK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.212"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(192..1784)
                     /gene="hcp"
                     /locus_tag="KPK87_003566"
                     /gene_synonym="priS"
     CDS             complement(192..1784)
                     /gene="hcp"
                     /locus_tag="KPK87_003566"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG2539670.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1844..2569)
                     /locus_tag="KPK87_003567"
     CDS             complement(1844..2569)
                     /locus_tag="KPK87_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2539671.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2734..3438
                     /locus_tag="KPK87_003568"
     CDS             2734..3438
                     /locus_tag="KPK87_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2539672.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSTCVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            complement(3602..4873)
                     /locus_tag="KPK87_003569"
                     /pseudo
     CDS             complement(3602..4873)
                     /locus_tag="KPK87_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         1690 a          896 c          618 g         1926 t
ORIGIN      
        1 aaatttaata caaagattct taaaaaaaat atactgaaag caggtttatt tttcaattat
       61 tacaaaaaat aaataaaggg tcatctcaaa attgattttt taatcaaatt ttaagtttaa
      121 ccccttatct taattgtggc atacttaaaa atagtttaac tataaatatc tttaagtata
      181 gatatattta tttatcctaa aatcttcttc atatcttctt caggagtaga tataggtgtc
      241 aatgcaaatt tatcaaccaa tatatctaat acattcttag ataagaatgc tggtaaagaa
      301 ggtcctatat agatattttt gatacctaaa tgaagtagtg taagaagtat acaaacagct
      361 ttctgctcat accatgacag taccatgcta agaggcaaat catttactcc acattcaaaa
      421 gcatttgcca atgaaactgc cactttaatt gcactataag catcattaca ttgtcccata
      481 tccattaaac gaggtaagcc acctatatcc ccaaggtcta aatcattaaa tctatatttt
      541 ccacatgcaa gtgtcaatat aacagtgtct ttaggagctt gttttacaaa ttcagtgtaa
      601 taatttcttc caggttttgc accatcacaa cctccaacca ataaaaagtg tcttatagct
      661 ccactcttta cagcatctac tactttatct tgtacagatg aaactgtacc tcttgcaaaa
      721 ccagtagtta atgtatctcc tccattaata ccagtcatat gaacatcttc tctatatcct
      781 ccaagttcaa gagctttttc tattacacta gaaaaatctt tatcttcatc aatatgaacc
      841 atacctggat aagcaactac ttctgtagta aatactctat ctctataact atctttaaca
      901 ggcattaaac agtttgttgt aaataatatt ggagcaggaa tattatcaaa ttctttttgc
      961 tggttttgcc atgctgtacc aaaattacct tttagatgtt tatattcttt tagttttgga
     1021 tatgcatgtg ctggtaataa ctcaccatga gtatatatat ttataccctt atctttcgtt
     1081 tgttctaata atttgtgtaa atcataaagg tcatgtcctg taactacaat aaatggccct
     1141 ttctcgattt tcatagatac ctgtgtaggt tctggtgttc cataagtttc agtatttgct
     1201 ctatcaagca attccataca ttttaagttt atttctccag ttttcataac tagacctaaa
     1261 agttcgtcaa tagacaagtc tcctccaact gctattaatc cttcatagaa aaattcattt
     1321 acgtctttgt ctgtataacc aagtaccatt gcatgatatg cataggcagc catacctctt
     1381 aatccaaata agattaatga ttttaatgaa cgtatgtctt cattatcatt ccataaatcc
     1441 ttcatatcat agttatcatt tctattacaa gttgttgaac aatctgaaca tctaggtaca
     1501 agtttacttt tttctacctc agttttttca atttgtcttt ttatagcttc atcatcaaaa
     1561 cttacattcg ttatagtagt gaatagcccc tctatcataa ttttatctgt ttcacttgtt
     1621 ggtctattct tatttccaat agttgctctt gaaagaccaa tcaacactcc tgtaagttcg
     1681 tcttgtaagt tagctgtacc ccccttttta ccacagacac catttatagt acaaccttta
     1741 cctcctgctg tttgttcaca ttgaaaacaa aacatttttt ccatttttta ttcctccctt
     1801 tttatttatt aaaatttatt atatttttct ataattcact taattaaatt tctccatcta
     1861 caatttttcc atctacagat atagtaacta tttgccatgg tatcattttt ccactatttt
     1921 gtagagctgt cttaacagca tttactatac caccacagca aggaacttcc atacgtgtta
     1981 ctactatatt tttaatatca ttttctttta aaatctctgt aagtttttca gcatagtcaa
     2041 cttcatcaag tttagggcat ccgattaatg taaccttatt tttcataaat ttattatgaa
     2101 aatttccata tgcatacgct gtacagtctg ctgctattaa aagacttgca ttctttaaat
     2161 atgatgcatt agttggtact aattttattt gtactggcca ttgattaagt tgagaacctt
     2221 ttatctctac tatatcattg cttatagacg tactatttga aacctctcta tttattgact
     2281 tagactgaga acctggacat ccacatgcta aagttttctt ttgtgctttc tttgcttcca
     2341 tatttgcttt tactgcagct tcatcatatt ctgctgcttc tctatactca aatgaaatcg
     2401 catttgttgg acaagttggc agacaatctc ccagcccatc acaatagtca tctcttaaaa
     2461 gttttgcttt tccatttacc attcctatag cttcttcatg acaagcttca acacatagtc
     2521 cacaaccatt acacttttcc tcatcaattt taattatttt tctcatcatt tatatcacct
     2581 cataaaattt ttttaattta aagtattgat ttcttatata tttattttta atacctatat
     2641 ttctttattt caaatttctt gtagtaggat ttttatatcc ccttgctttt ataaacttag
     2701 tatagtacaa tgaaaacaaa ttatcggttg catatgcaac aaggaagtga ttatttgaaa
     2761 aaatttttag aaaagcatat agacctactt aaaaaaattc ctctatttga aggaattaaa
     2821 agtgatgaac ttgaagaaat gttggaatgt ttaggtgttg tagataaaac ttacccaaaa
     2881 gatagtataa tattctctgt tggaagtgaa gttacttcta ttggaattat gcttaaagga
     2941 agtgctcata taataaagga agatatcgaa gggaatagga atattgtagc tgaactcttg
     3001 cctggagatt tatttggaga ggtattcgca tgtactagac tacataaaag taatgttaca
     3061 gtaaccacaa catcttcttg cgaagtttta tttataaagt ttaaaagtgt aactggtatt
     3121 tgttcatcta catgtgtatt tcataatagg cttgttgaaa atatgctaca attaatagct
     3181 gaaaaaaaca ttttacttca caataaaata gaattacttt ctcgtaaaac aactcgcgag
     3241 aaattgctta tgtatttttc aaaacagata gaacaaacag gtagccacca gtttacaatt
     3301 cccttttcaa gaaatgaaat ggctgatttt ttatgtgttg acagaagttc tatgtcaaga
     3361 gagcttggca agatgcgaga tgaaggcttg ttatctttta acaaaaataa atttgaaatc
     3421 ttttatttta aaaaataaaa tagatattta aacatattta tttcaatata tactatatta
     3481 gttgattcaa attataaata tcatatttga atcttaaatt tatattttta cttaaaaagt
     3541 taataggtat ctaatttatt aaaattaaga tacctattaa ctttattaat taagtgtaca
     3601 attagcttat tttctttgta gtaattgctg ttttatctat aagccaatct tttccttcta
     3661 ctaatcttga aactaacatt gatgatactg tatttccagt tgtatttaat atagtattag
     3721 gaatatcagt tatagtagtt ataactgtta acagtggaat tgcctccact ggaaaaccga
     3781 agatgctaac cacgagcatc tcaccaataa gtcagccacc tgctacagga gccataaata
     3841 ctccaacaac aaatacagtt ataaataatt gacctatcgt tgctggattt gccattccac
     3901 tacctaaaat acctgataaa aatgccactt ttattattac tgctaaaact gacccatctt
     3961 tatgtatatt tattccaaga ggtataactg tttctgatat atcatcagga actcctatat
     4021 ttttactagc ttctaaattt gatggtatac atgctgaact tgaagaagta gctagtgccg
     4081 ttgctattgg ttttatagca tttctccaga attgtctaaa cccttgtttt cctgcagata
     4141 aaaaagcata cagactgtaa aataataaga aatacatggc tgctgttagt gaataaatta
     4201 caaacaccct tacatatcct tgaattattt tacttccaag ttccccaatt attgtagctg
     4261 tataacagcc tagccctata ggagcataaa gcatgaatat acttactact tttaatattg
     4321 cttccgttcc ttctgataaa agctttctta ttgtatttcc cctatctccc atcatagcta
     4381 ttgctgttcc aattaaaact gcaaatataa ctaattgtag taaattacta ctggaaaata
     4441 aatctccaaa atttttaact gtaatacttg atactacttg gtctaataac gttccttttg
     4501 attctaaagt ttctgcatct tgagccacca ttaaattttt tacacttgat acatctaatc
     4561 ctttagacgg attaaataat aatgcagatg taatacctaa aaatgcaatt acaagtgttg
     4621 ttcccataaa tactatagct gtacccttca ttatttttcc aaatcgttcc atatttttta
     4681 tactagcaac acctgaagaa acactaagaa aaactaatgg tactactatc attaatagag
     4741 catttatgaa taaatctcca aatggtttta gtattgaagc ttttggacca agtactaatc
     4801 ctactatcgc ccctactata gtacatgcta aaagtataat tgatgactta taatttttta
     4861 aaaagtccct catttatatt ttcctttcta atgctttata ttataaatta ccccaaatgc
     4921 taaaaaaatt aaagtaaatt atctaatgcg cattaataat ttttgtaatt ttccccaaca
     4981 gctattttag caaatactgg aatatatgtc aatttctata gtttttttgt atttttaaac
     5041 gttttattaa tagttcatat ctatatataa atattaataa aattctgctt ttaaaacttt
     5101 ttttctagat taattttaat aaaacaaaca
//
LOCUS       DAEMIK010000210         5086 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEMIK010000210 DAEMIK010000000
VERSION     DAEMIK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.213"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            274..1581
                     /locus_tag="KPK87_003570"
     CDS             274..1581
                     /locus_tag="KPK87_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539673.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKVPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1667..1929
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2055..2858
                     /locus_tag="KPK87_003571"
     CDS             2055..2858
                     /locus_tag="KPK87_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2539674.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2896..3507
                     /locus_tag="KPK87_003572"
     CDS             2896..3507
                     /locus_tag="KPK87_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2539675.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3539..4210
                     /locus_tag="KPK87_003573"
     CDS             3539..4210
                     /locus_tag="KPK87_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2539676.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4509..4871
                     /locus_tag="KPK87_003574"
     CDS             4509..4871
                     /locus_tag="KPK87_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG2539677.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2054 a          461 c          891 g         1680 t
ORIGIN      
        1 tagtgtgtaa tttgataata tgtaattata acattatgat ataattacaa taaagtatgt
       61 aataatctgt agaaatttca agctagttat ctaagtcgga taactaaatc taaacgaaat
      121 acataatgta ttaaataaat gatgatacta aaatgatact atttttatag aaatttattg
      181 aggtatataa ttaatgaaaa taatatatta aattatataa attaaattat ataatatatt
      241 gagttttaca taaaagcaaa ggtgggaata aaaatgaaag aaattgctga tgctataaca
      301 tcagagtatt taagctatgg tatcaatgaa gatattagta tctatgaagg tagattttgc
      361 atatatttag ataaaaaata tagatgtaat gggaaaattt attataaaat gacacctcca
      421 atatctataa gttttaaagc agacataggt tgtgttgaag agatagacaa tgaagatgac
      481 aatttagctt tagattatga taatgctatt cttgaagttc atggatataa aattatttct
      541 ataacaataa atactttaag tgaatttagt gtagaaggtt atatcaatga tgactgcata
      601 aaatctaaga actcctatgt tgaatatgta gattttaata taataaactt agataaaata
      661 cctggaaaac tcattaaata caatgataag gtatacgcag gtagaattga atttgatata
      721 aatgattatg ttgtaactat agacaagcga tatgattata gaaaagaatt aaaatcagaa
      781 ttaaagtcta aatctggagc aataataact catataggta ggattagaag aaaagatggt
      841 agaattttta gaacaaataa tacaataaat cttttagata ggatatcaac agctttgagt
      901 tttatgtgtg gaagatatgt agggttttgt ttggccaaag ggtatagaag tggtaatgaa
      961 gtttatagaa tatggaatga aaatcaaata tctcctttta gatatgtacc aacatggtca
     1021 gatacacttt caaattatca taatatggaa aaatatataa gccttatgtg taaaaagcta
     1081 gaagattttt attatggttc tgctataaag agtgttgttg attggtacat agaatctctt
     1141 ggaagtgcta ccatggaaaa caatataata tctgtacaaa ttgcacttga aactttgtca
     1201 tatgtaattc tagtggaaca aaataagata cttacagatg aagcatttga ctgtaatttg
     1261 gcatctaaaa atataagact tttacttgat acatgtaagg taccatatgg taaacatgaa
     1321 ttaaatatat ttgataatat aataaaaaat aaatttgatg atggagtgga tttagtcatt
     1381 tatttaagaa atagcatagt tcatccaagt agaaagactc atagagctgt gttagaggtt
     1441 gaagatattt ggaatataat ttcaatagga acgagatata ttgaacttgt tttactcttt
     1501 attttaggtt atagaggaga atattcaaac agattagtag aaagatgtta tggtgaagtg
     1561 gaagttgtac cttggaacta gatttttata tttattttaa aatatatatt gacatatatt
     1621 gccttatgct ataaaatcaa tatcataata ataattcaga ataacattaa atgctaagaa
     1681 aagaaatagt aaaagtagtt aatcttaaac agagagtttc cgattggtga gaggagcaat
     1741 agattttact ttgaacacat ctttgagcag gactctgaaa gagtagaaat actttattag
     1801 gagatttccg gaatccttgc acccgttata gtgctagagt ataataattt tattgtactt
     1861 aaagaggtta gtgtcgtgag gcattaatga acagaggtgg aaccacgaga gttaactccc
     1921 gtcctctagc agaagctaga ggcgggagtt ttttaatata attttttaag cgcgcagaga
     1981 attatattgc aatagataca ctttttaaaa aataatcaaa atattttaaa aattaaaaga
     2041 cgagggagaa tattatgaaa aatatattaa aaaaagtagg aatatttaca attatgttag
     2101 gtttattagg tggagttgta ggttgttcaa aaccagataa tgaaaaagat aaggatgctt
     2161 caaaggaatc taaaaaagaa gtagttgtag ggtttgataa tacgtttgta ccaatgggat
     2221 ttttagatga aaagggagat acagtaggat ttgacgtaga cttagctaaa gaaacattta
     2281 aaagacttgg aatggaagtt aagtttcagc ctatagattg gtcaatgaag gaaactgaat
     2341 taaatgattc aaaaactgta gatgtattat ggaatggata ttctataaca gacgaaagaa
     2401 agaaaatagt atcatataca gaaccatatt tacaaaataa gcagataata gttactctaa
     2461 gtgactcaaa aataaattca aaagctgatt taaaagataa agaagttgga acacaacaag
     2521 gttcaacagc acttgatgca gtggaaaagg ataaagattt tatgaatagc ttaaaaggtg
     2581 gagcaccagt tctttatgat acttatgata aagcacttag agatttagag ataggtagaa
     2641 ctagtgcagt tgttggtgat gaagttttaa ttagatacta tatgggacaa aagggtgaag
     2701 acaaatacaa ggtattaaaa gacgattttg gattagaaga ttatgttgta gcaacttcta
     2761 aagaaaatcc agaactttgt gagaaaataa atgaaacatt aaaagaaatg aaaaaagatg
     2821 gaacttttga caaaatttat gataagtggt ttaaatagaa gataagttag ctgttaagaa
     2881 gggggcacta aaggcatgtt acaaggttta gagatagtta tagcaatgtt ttgtataaca
     2941 ttgatagtat caattccatt ggggattgga gttgcatttt taagattatc aaagaataaa
     3001 ttagtaagtg gtattacaca atgttatata ttgataatga gaggtacacc tttacttttg
     3061 caaatgatag tcatatttta cggattacct cttttaggaa ttgtatttga cagatttaca
     3121 gctggagtag tagctttctt cttaaattat gcagcatatt ttgcagaaat atttagaggt
     3181 ggaatacagt caatcgatag aggacaatat gaagcttcaa aagtactagg atttgataag
     3241 tttactatgt ataagagagt aatctttcca caagttttta aaagaatact agcacctatt
     3301 tcaaatgaag taatcacatt agtaaaagat acatctttag tatatatatt aggattaaat
     3361 gatattttaa gaatatctca aatagcaatg aacagggaag caagtctgtt accattattt
     3421 gaggcaggag cgatatatct tatatttgta gctatactta ctaagggatt tgaattactt
     3481 gaaaaaaaat attcttatta tagataaagt tacaatatta taggaggagg ttttttatat
     3541 gttgaaaatc aaaaacttaa ataaatcatt taaaaaaaat agagtactta aagatatttc
     3601 ttttgagtta gaagaaggtc agattggagt tttacttggt aagtctggtg caggaaagac
     3661 cacaatatta aggtgtataa atggtcttga agaatttgat agtggagaga ttataataga
     3721 taatgaggta ataaaaaata agagagatat ggcaaaaata cgaggaaaaa taggtatggt
     3781 ttttcaaaat ttcaacttat ttcctcatat gacagtcctt gaaaatataa ttgaatcccc
     3841 agttaatgtt tttaaagttc ctagaaaaga ggctgaagag agggctagag aacttttaag
     3901 attagttgat ttagaagata aattaaattc ttatccattt gaattgtcag gaggacagca
     3961 acagagagtt gctatagcta ggtcatgtgc attgatgcca aaagtccttt gttttgatga
     4021 acctacttct gcacttgata ttgatactat tcaaagagta gtcaatataa tgaacagact
     4081 taaagacaaa ggtatgacaa tattaataat tacacatgat gttgtcttct caaataatgt
     4141 tgcagataaa attataagta taaaagatgg tatagtagaa aatgttcaaa taaaagaaaa
     4201 aattgtttag agttttataa atataaccta gggtagaatt ttataatata aataaagggc
     4261 aagtaaaatc aaatatacta tgcccttttg ttatctcaaa atttaatctt atattttatg
     4321 tttaagttac attataattt ataataaaaa taaaaggact atttaaatat ataatgtgaa
     4381 taaatgttta ataatttgaa atatgataat tttttatgtt gaaagcttat aatgattatg
     4441 atataaattg gttatagtat acaatttatg ggtataacaa taaaaaatta gaaaggaatt
     4501 tttataaaat gattaaatta tatggatata caaaatgctc tactgttaaa aaagctaaaa
     4561 attggcttaa ggaaaataat ttagaatttg aagatataga tatggttcaa aatcctccta
     4621 gtaaagagga gttaaaatca atttataaga ctagtggata tgatataaag aaatttttta
     4681 atacaagtgg tatgaaatat agagaattgg gtcttaaaga tattgtaaaa actgaatctg
     4741 atgataaact tttagaaata ttggtaagtg atggtatgct tattaaaaga ccattacttt
     4801 tggatggtaa aaatgtatta ttgggcttta aagaagatgt atggaaaagt actttgctaa
     4861 aagaagatta gaaagagata ggtgtgttga ttaaaattat taattaatac acctataaat
     4921 ttatgtatta aatctaattt ttataaatat taattagcaa attcaaatca aaaaaattca
     4981 tatcaacaaa catattactt cttattacaa attatatcat atgttttata ttaaaatatt
     5041 ataatattac aacgaattgt tacaaaatta cttattatta accatg
//
LOCUS       DAEMIK010000211         5021 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEMIK010000211 DAEMIK010000000
VERSION     DAEMIK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.214"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(124..843)
                     /locus_tag="KPK87_003575"
     CDS             complement(124..843)
                     /locus_tag="KPK87_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG2539678.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(961..2235)
                     /locus_tag="KPK87_003576"
     CDS             complement(961..2235)
                     /locus_tag="KPK87_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG2539679.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(2435..3802)
                     /locus_tag="KPK87_003577"
     CDS             complement(2435..3802)
                     /locus_tag="KPK87_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2539680.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(4052..4516)
                     /locus_tag="KPK87_003578"
     CDS             complement(4052..4516)
                     /locus_tag="KPK87_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG2539681.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(4521..>5021)
                     /locus_tag="KPK87_003579"
     CDS             complement(4521..>5021)
                     /locus_tag="KPK87_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBG2539682.1"
                     /translation="FILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKI
                     NESMKKVIYKNKEEDNVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFI
                     GENCKGIEKVKRIKAVLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEV
                     IEQINS"
BASE COUNT         1665 a          924 c          513 g         1919 t
ORIGIN      
        1 ttgtagctaa aattataagt gctaaaacca tatatatagt cattcctttt ttaaaataac
       61 cattgtcata ataattcata tagataaact tatttttact caaatttttt cactcccaag
      121 attctaaaaa tttatatcaa aaaattctat aatttcatct tcatgataat ttatgactaa
      181 ttcatctgga aaatctactg cttctaaaac tttttctgca ttttcaaatt ctcctataga
      241 ataacatata tgagagtcag taccaagaat tactcttgca ccatatgttt tacacaatgt
      301 caacatatga cttacatttt cccaagtacc cgttctatga gagtttgaac ttaatgatga
      361 attatttatt tctaaaagga tatttttatc tttagctttt tttactatag gttcataatc
      421 taaaggatac ctactgtcat ctggatgtcc aattattttt accttaggtt tatccataac
      481 ttttaaaata gcatttgtat tttcttcttt tgtaccagaa gctatacaag gtctatgcaa
      541 acttgcgatt atataatcca aatgttgcaa catatcttct tgaacatcta aattaccatt
      601 ataatccatt atatttcctt ctattcctct aagaatcttt acgccttgca cttcacgtgg
      661 aagcaaatgc aaattataaa aatagaatgg atgtggtcct cctggcatat taggtccatg
      721 gtctgatatt cctaagtatt ttatattctt cttactcgcc tcttcgacat tttccttgaa
      781 agtactatgt gcatgtccac ttattagagt gtgacaatgt acatccataa tagacttttt
      841 cattaaattt cctcctaata ttttacataa attcgtgaaa atagctaaag ggctatttca
      901 aaactaaagc tgtcataata gacagtgtaa cgttttaaaa cagccccatt ttcaattatt
      961 ttacacttct aaatctttaa atttatcttt aaataattct ccaatacttc ctaaagattc
     1021 ctctacttct aggtattctg ttatatcctc ttctggctct ctagttgctt cttttattga
     1081 aaccaacatt ctcttagtct ttggtttaaa atcaagaatc ttaactttaa cctcatctcc
     1141 aatattaaca acattagtaa ctttagccac tctagtatca ctcaattcag atattggtaa
     1201 atacgcatct acaccttctt taatttgaac aaaagcccct ttttctatta ttctaagaac
     1261 tttagcattg acaacatcat caatttgtac ttcttttgct attaaatccc aagggtcatt
     1321 atttatgtct tttaatgcca aggcaatttt tttagtctct ttatttacat ccaacacata
     1381 aacttctact tcttgtccct ctgaaacaac atcttcaaca ctttcaactc ttgtccaagc
     1441 taaattattt atatgtgcta atccttcaat cccaccaata tccacaaatg caccataagg
     1501 cattatcttt gtcaccttac catctctttt ttgtccaact tcaagagaat taaataagtc
     1561 ttctttagct gctttgatac gagctttttc ttcttctctt tcttgcttaa ttcttgctct
     1621 ttcagcttct ttttctgcct taatttgagc tcttctttct tctctctctt tatttatacg
     1681 ctcttgtaat acatctctat gagatgctac aagtctattt tttctaacat ccaattcttt
     1741 tatataaact tctaagttaa gaccaacaaa tttagatgta tctgtaataa attttgtatc
     1801 aagttgagaa attggtataa aaaatgtata tgtattgtaa ttagcaaata cacctttatc
     1861 tatattcttt tcaacattta cagttattat tctatgttca tcataagcct tttgtaactc
     1921 ggcaaaatca tttcttttgt caagttgtaa tttagataaa gttattgtac catctttttg
     1981 agatactttt gttattacag cagttatttc atcacctatg tggtatatat cttctatata
     2041 ttgatttttt tctatgttca attcacttat aggtattatt ccatcaaacc catagtctaa
     2101 tcctaattgt acttcatcat ttctcactgc agttattttt ccagttgtca attctccaac
     2161 tttcactttg ctgaatactt gttcttgctg gtcaagtaat tcttgcattg ttaaatcatt
     2221 ttccattata aacatccttt cctcttattg attatcatat ctgtaaatat tataccatta
     2281 atattgcaaa tacttacatt tttcttacta taatttttaa aataaattca acttcatata
     2341 tttatacatt atagaaaata tcttattaaa ttaaaataca aattctaatt tttatctaat
     2401 aaaatatttt cataaaaaat ttatattgta tatcttattc ttttattgct ttaggttttg
     2461 atagaaaaca tttataaaat ataaagaaag aaattgctgt tatagtagca gctgttatat
     2521 ctgcaactgg ctctgctaaa attactgcaa aaagtttttc attaaagaat gttggaagta
     2581 taaatattaa aggtattaaa agtacaatct ttctaagtag agctaaaacc aaagatattg
     2641 ttgcttttcc aagtgctaaa aatgtctgtt gacaagctat ttgaatccca aacataaaag
     2701 caccagcaaa atatattttt atactccaac tagtcatact aagtaactgc ggattcttat
     2761 taaatatact aacaaaaaat gcaggaaata tcattaataa aagccataaa atagtagaat
     2821 atgtaaaaca tgacaacaaa cacaacttaa atgtcttttt tactctatca agttgacctg
     2881 aaccataatt ataacttatt attggttgtg ctccttgtgt aacccccata ttaggaagca
     2941 taagaatttg cattatactg ctcattatag ccattgaacc tactgctaaa tctcctccat
     3001 acatttgtaa tttactattt aaggctataa gtactaaact ttctgtagat tgcattataa
     3061 atggagagat tcccaaacca agcactggta agatgatact tgcttttggt atcatatact
     3121 tcttttttat tttaagtata cttttctttc caaagagaaa cataagtacc caaatagcag
     3181 aaacgccttg tgcaattata gtagctaatg cagctccttt tacacccata ttaaagccaa
     3241 atataaaaat cgggtcaagt attatgttta ttaaagcacc tatagcaact gtcatcattc
     3301 cagttgtagc aaacccttgg gtatttataa aagtattcat acccattgaa atctgaacaa
     3361 acactgtacc tattaaataa atatttaaat agtccattga atatccaatt gtagattcac
     3421 ttgctccaaa catccaaagt aaaggctctt ttccaatctt aaaacctata gtcaaaagta
     3481 ttgctaaaat caccagtaca gaaaaactgt tactcattat tttctctgcc tcatcattgt
     3541 tttgctcacc catttttata gcagctaatg gacctcctcc cataccgatt aaataactac
     3601 atgcagttat tataattatt attggaaatg ccactccaac accagccata gcaacttctc
     3661 cattttccat tctaccaata aaaatcctat cgactatgtt gtataaaaca tttacaattt
     3721 gtgcgataat agcaggtgta gcaagtttaa ataataattt acccacactt tcactaccta
     3781 agttagtgtt tgaattttcc attttaaaac ctcctcaatc caataattaa aatgtatcac
     3841 taaaatttta ttctatataa aaatttcatt ttattagcag acaaatttag agtactttcc
     3901 ctacaagtaa tactatacta tatttctttt gccatagtaa acattttaga actaactgaa
     3961 gttaaatact ttatctcatt ttttatttat tattaaaaat ttttcacaca tactcataaa
     4021 aaaactatcc aagtatatta cttagatagt tttatattat atgtaaagaa agagctgaaa
     4081 ctataacccc tgataacaaa ttgccaagaa atactcccat aaaagcatct tttattctca
     4141 tttttaatat ggaagctata gcagatgcag tccacgttcc tgtagttggc agtggtatac
     4201 ctacaaatag tattatacca attatcgaaa cactttttaa tttcttcatt cttgagttta
     4261 tttttctatc aattacatca gcaatccctt taaatagttt cttccctctt aaaaattgta
     4321 atacatgtct gaatgtcaat atcaagggaa tcgataccaa tgtagaacct attacactaa
     4381 atacataaac ccatatagga tttaagccca ttgcaatacc tataggaatg gctcccctca
     4441 attctgttac aggaaccata gataaaatta atatgtgtaa atatcccaaa ctattccctc
     4501 ctattctaag tgacattaag ttatgaattt atttgctcta taacctcacc atccttttta
     4561 ctaactaaaa atgcattttt agtcaatgaa agcattggta aatcactctt agagtctgaa
     4621 tatgcatatg aattttcata atcaatctct atcccaagtt tacctaatac agcctttatt
     4681 ctttttactt tttcaattcc cttacagttt tctccaatga atttattttt atatcttgaa
     4741 ttttcataaa aaaactccgt gcctataacc tcgtctgcaa gaccataata cttaaaatat
     4801 ttcatatatg catatggaga tgcagttatc ataattataa cattatcttc ttctttattt
     4861 ttgtaaataa cctttttcat agattcgtta atttttttca ttatgcaatc atcaaaaaac
     4921 ttttttaaat cttcttcact aaaatctact attggataaa agattgcctc ttttgccttc
     4981 ttcaatggta ttattctaat taaatacata aatagaataa a
//
LOCUS       DAEMIK010000212         5011 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEMIK010000212 DAEMIK010000000
VERSION     DAEMIK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.215"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(21..815)
                     /locus_tag="KPK87_003580"
     CDS             complement(21..815)
                     /locus_tag="KPK87_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539683.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(816..1691)
                     /locus_tag="KPK87_003581"
     CDS             complement(816..1691)
                     /locus_tag="KPK87_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539684.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIVLNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(1695..2063)
                     /locus_tag="KPK87_003582"
     CDS             complement(1695..2063)
                     /locus_tag="KPK87_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2539685.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(2245..2904)
                     /gene="deoC"
                     /locus_tag="KPK87_003583"
     CDS             complement(2245..2904)
                     /gene="deoC"
                     /locus_tag="KPK87_003583"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG2539686.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(2940..4127)
                     /gene="murJ"
                     /locus_tag="KPK87_003584"
     CDS             complement(2940..4127)
                     /gene="murJ"
                     /locus_tag="KPK87_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG2539687.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(4204..>5011)
                     /locus_tag="KPK87_003585"
     CDS             complement(4204..>5011)
                     /locus_tag="KPK87_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2539688.1"
                     /translation="KKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRI
                     LNATISKEPSDRYYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYL
                     KKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKEND
                     IICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQIC
                     NKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         1655 a          849 c          501 g         2006 t
ORIGIN      
        1 ttaagcaggt tttattttag ttataataag tcatttgcat actctttaat tggtgctttt
       61 ctaagtaata aaattataaa tattatgcaa ataactataa ttcctacttt tgttccaaat
      121 aaaataaaag gattctgaaa tgaataaata attttttcta aatacaatag cattttatgc
      181 atatttttag ttgcaaaatt aactatataa attatcatta atatcggtaa tcctatcaat
      241 atagtaatac tagtaacttt tctaactcta tatgtaaaag ctccaagtaa cactcctata
      301 aaacctatac atatttcatt tatacatatt tgtacaaaat aatttaaata agttagtggc
      361 atacttgatg ttgaaggatc tatcagtgct gatatagatg tccaaatcat atctctcttt
      421 agtaaaactg atagttctat actgtaacct gttataattt ctataaataa cttcgataaa
      481 aaaatcaaaa ctatacctaa aatagaaatc acaaagctta aaataattaa atctatgaca
      541 atagctttta tacaactttt tctatctgct cttatactta aagctcctga aaagtcttta
      601 tttgctatat ttactccata cacaaatagc agtatagtta tatatgttaa aataggattc
      661 acaatcgtgg ataaattagt tttagatgct tctccaaaaa tcttgagaat aaacactggc
      721 aaaatattca taacaattcc cacaatcata aatataatta tatacaattt agtattttta
      781 ttaaaaaatt ttatatatgg ctttaattca ttcaattaca attcctcctt cttagtcatg
      841 ttaacaaaca tatcttgtag ccctatgtat cctacatcaa tatttgaatt tttaagtatt
      901 ttttcatcac tttcactaaa atcaccatag taaaaataag caactgcatt tccaaatgcc
      961 tttttaggtt ttatattttt tatacattct aatttttcta actcttcttt atttccagta
     1021 atataatgag ctttttgttt tatgacatca atttcttcat ctataataat ttttccctca
     1081 tttaatacta ttacatgctc cagtaaatca tctatttcat caatcaaatg agttgatatt
     1141 attattgtcc ttggattttt tatataacta tctaaaatta tgttataaaa ctcttgtcta
     1201 tttactgcat ccagaccaat tgttggctca tcaaatatgg tgatagctga attagaacaa
     1261 atacctatta tatttgaaag tatagttttc atacctcttg acagttgttt atactttttt
     1321 ttagtattta aatcaaaata tttgcataat tctttttcta agcctttatc ataactttta
     1381 taaaaactag aagaaaattc aaatatctga cctactttaa aatctggact ataaaattct
     1441 ttttccctga caatacaaac ttcttccaaa acttttatat cttcttttgg gttctttcct
     1501 aatatttcta tctcaccttc attttgaatt aactgattta ccattatatt taataatgtt
     1561 gtttttccag ctccattctt acctaataaa ccatatatct tatttttttc aaagtttaga
     1621 gacatattat taaaaatatt ttgcttctta aaactgtgag ataaattttc tatcttaata
     1681 gaactactca tatattaatc ctccttgaaa ttaattataa tatcaaccaa ttcttctcta
     1741 tttatttcta atttgttagc ttcttgaagt aggtttttta caaaattatg tttaaaattt
     1801 tcttttctag cttcttttat aatagttttt gcaccatctg atacaaacat ccctatcccc
     1861 ctcttcttat ataaaatatt tttatcaacc aatatattta taccttttaa tactgtagct
     1921 ggattaattt tataaagctt tgaaagctct gttgttgatg gaacctgttc ttcttctttt
     1981 attccatttg atagtatctc atcttcaatt gctcgtgcta tctgaataaa tataggttct
     2041 tcattattta atactaattt cataggtgcc tcctttctcc attagttagt tactcaacta
     2101 attaactata taacttataa tatcttgaaa aatattttct gtcaatacta aataaaaaac
     2161 agagggaaaa ttccctctgt ttgttgttct ttatatttat aattaaattt taatttttgt
     2221 aatcctatcc ataagttaaa aactttaata atctgttgta tcttcatttt ttaatatttt
     2281 acatattgag ctagttccta acctttctgc tcctgcctct ataaattttt ctgcatcaga
     2341 aattgattta actccccctg cagcttttat ttttacattt tttccaacat gctctttcat
     2401 aagctttata tcttctaaag tagctccacc tgttcccatt ccagtagatg tttttataaa
     2461 atctgaacct gacattgtta ctatttcaca catttttatt ttttcatctt catctaaata
     2521 acatgtctct ataatgactt ttaatacttt atctcctata gcttttttta tttcttttat
     2581 ttcttttcct atattatcat aatctttatt ttttatatct gaaatgttta caaccatatc
     2641 aacttcatct gctccatttt caatagcatc ttttgcttca aatacttttg tagcagtagt
     2701 ttgatatccc aaagggaacc ctatcactgt acatatcttt attttacctt ttagatattc
     2761 tgatgctctt ttcacatatg atggtggtat gcaaactgat gctacactca tatctacagc
     2821 ttcatcacat aaaattttaa tatcttccca tgtagttgta gcttttaaaa tagtatggtc
     2881 tactgttttt aatatatgtt tcataaaatc ctcctaaatt tataattaat ataattagat
     2941 tactttttta aatgcaattt tctctttgca atattcagta tcatatcaac ctcttcaact
     3001 ttaaaaataa tcattagtag tgtataaatt ccacctccaa ctataactga aatagctagt
     3061 gatacaaact cattaaatgt tcctactccc aaaattccaa atataaattt atacgtaaag
     3121 tatgataaaa cacccataat taaagatgca actaccgatt tcaaagttgc ttttattatt
     3181 ttatcttgtc caaaatatcc aacttttctc tttaggttca agaagagtaa caatatacat
     3241 gcaattgatg atgaactagt tgcaagagca agaccaccat gagccattgg ttttattaaa
     3301 actaagtcca atactatatt tacccctaca gatataatcc catttaccat aggtgttttt
     3361 gtgtcttgta atgaataaaa aacctttccc aaaatatctc ttaagccaaa tgcagtcata
     3421 ccaactgcat aaaaaattaa ggcagttgct gtcatttggg ttgccctcgc atcaaaagca
     3481 cctctctcaa aaatgattct aacaacagga gttgcaaaaa aaatagaagc aactgaaata
     3541 ggaatcatag aaattattat catatttata ctagatttaa ctgaactagt aaacttcttt
     3601 tgattatttt ctgcacttaa cttagataac ataggataca taacagatac tattgaagct
     3661 gtaaatgtcc ctgtaacaaa accatttaat ctatctgcat aagtaagtgc tggaatgctt
     3721 cctttaacta atgttgaggc taaagttgta tctaccaatg agttcacttg attaacagca
     3781 acacctataa atactggtga aagtaatgct agtaatttaa taaggctatc atctttaaag
     3841 tttaagtagt ataagtattt gtagttcgtt tttttaacaa aaaacatgta aaagagcagc
     3901 tgaactacca tagcaaccac agcaccaatt ggaagtatgt atggtccaaa tacggtactt
     3961 aacattattg aaataattat tactatatta tagggtatac ttccaaatcc aactacaata
     4021 aagttctctt taatttgtaa aaatgctgac ataacacttg taatacctat gaatattatt
     4081 cctgttctga acctcccatg actaaagtca cagggttcct gcttcataga attttgcata
     4141 ctaaaatatg tcttactaat tctccacagg ctatccccgt agttcctacg gttcttacat
     4201 aagttattta ttttgaatcg ttattaatct taatccttct tttaatatat ttatacttgc
     4261 atttatatct ctatcgtgag tttcattaca actaggacaa atccattctc ttatatttaa
     4321 gttcttaact tcctcgtttt tatacccaca tttattacat atttgtgaac ttgcaaagaa
     4381 tttacctacc tttacaattt gtctaccatg ccaattagcc ttgtattcta attgacgaat
     4441 aaactctgac catgatacat cagaaattaa gcgagatagt ttacgatttc taatcatatt
     4501 ctttacttgt aaatcttcta tgcaaataat atcgttctct tttattaatt tagtagatag
     4561 tttttgtaga aaatcatttc tttgattagc tatatgttct tgaagtcttg caacttttaa
     4621 tcttgcttta tttctattta aactaccgat tgtttttctc gataattctc tttgtaattt
     4681 agcaagtttg tttaatgact tcttaagata cttaggattt tctataaatt ctccacaact
     4741 tgagatacaa aattctttaa ttcctaaatc taaacctatc tgattattag tattttcaaa
     4801 tgcttcaata tctacatcag tacagcataa tgatacataa tatctatcac ttggttcttt
     4861 agatatagta gcattaagta ttctcccttt aggtacttgt ttatccctta ccttaaccat
     4921 accaagttta ggtagtttta tatgttgacc acaatacata atgtttccat ttgtaaagtt
     4981 agttttatat gaaaatctat taattttctt t
//
LOCUS       DAEMIK010000213         4914 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEMIK010000213 DAEMIK010000000
VERSION     DAEMIK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.216"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    188..419
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            527..2008
                     /locus_tag="KPK87_003586"
     CDS             527..2008
                     /locus_tag="KPK87_003586"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG2539689.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELTLAIKKFLKV"
     gene            2018..2911
                     /locus_tag="KPK87_003587"
     CDS             2018..2911
                     /locus_tag="KPK87_003587"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG2539690.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3035..4096
                     /locus_tag="KPK87_003588"
     CDS             3035..4096
                     /locus_tag="KPK87_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG2539691.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4251..4763)
                     /locus_tag="KPK87_003589"
     CDS             complement(4251..4763)
                     /locus_tag="KPK87_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG2539692.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1830 a          612 c          765 g         1707 t
ORIGIN      
        1 aaaaacagct tcatcactat gaaaatctag gtttttaaac ttttaaaaaa gttaaaaaaa
       61 tatttttcta atactcaatt tgattacaat ttttttacaa tttaaaaaat attttttcca
      121 aatttttata ttaatacttg actttgtctg ttttataaac tagaataatt aataatatta
      181 agaatattga aaggcattga ttaggaagag taaatctgaa tcgattacca gagaaggaaa
      241 ctactagctg aaatgtttcc ctaattagaa ggattgaaaa ctacctagga gcttctatcc
      301 gaagttatct taatgatttt agtaggaata gacgttttta ttacttgcgt taaagtatca
      361 agtggatttt ttaaaatcaa tttgggtgga accgcgggaa acagattctc gtcccttttt
      421 tagggatgag ggtctttttt tattacctaa attcagtttt taaaatattc tgtatctaat
      481 ttaattagct aattaaaaat ttaaaatata tggaggatta taaaaaatgt attattcaag
      541 tacgagaggc accgaagaaa aagtaactgc atcacaagca ataataaaag gaatatcaag
      601 tgatggtggt ctttatgttc caagtgaatt tcccaatgta aaaaatgaac ttataaattt
      661 agtaaattta acgtattctc agatagcatt ttttgtactt agtaaatttt tatgtgattt
      721 tactgaagat gaaattaaaa attgtataga aaatgcatat gatgaaaagt ttgattgtag
      781 tagtatagct ccattaaata aagtaaatga tacttatttc ttagaactat atcacggtcc
      841 aactttagca tttaaagata tggcacttac tataatgcct catcttctta aaacatctat
      901 aaagaaagat aatttagaga aagatgttgt gattcttact gctacatctg gagatacagg
      961 caaagctgca cttgagggat ttaaagatat agataaaata aagataattg tattcttccc
     1021 agaagatggt gtaagcccag tacaaaaact tcaaatgaaa actcaaactg gaaaaaatac
     1081 atatgtagtt ggtataaaag gaaattttga tgatgcacaa tcaggtgtaa aaaagatttt
     1141 ttctgataaa gaatttaatc aaaaactatt ggataataat tatatattat cttctgcaaa
     1201 ttcaattaat ataggaagac ttcttccaca agttgcatac tatttttatt catatatgaa
     1261 attagttagc aatagtgaga taaatttaaa tgataaaata aattttgttg tcccaactgg
     1321 taattttggt aatattctag ctggatatta tgccaaacaa atgggtcttc caataaacaa
     1381 attgatatgt gcatcaaatg ataataatgt tctttatgat ttctttaaaa ctggtgtata
     1441 tgataaaaat agaacattga aattaactac ttctccttct atggatatat taatttcaag
     1501 taatttagaa cgattactgt ttgaaatatc aaatagaaat actgatattg taaataagct
     1561 gttatcagac cttagtgata aaggagtcta caaaataagt gatgatatgg aaaaaaattt
     1621 agcctcattc tatggagaat attcaaatga agaagaagtt aaaagcacga taaacaatgt
     1681 gtttaaaaat tataattatt tgatagatac tcatactgct gttgcataca attgttatga
     1741 aaaatataaa aaagaaactt ctgataacac aaaaactgtt attgtaagta ctgcaagtcc
     1801 attcaagttt tctgaagatg ttctaaagtc tatagattgt gattttaaag accttgatga
     1861 ctttgctata attgatagat tatcaagtat ttctaaaata gatattccaa aacctattaa
     1921 aaatcttaaa aatgcagaaa tactacataa agatatttat gaaaaagatg aattaacact
     1981 agctataaaa aaattcttaa aggtataggt gattattatg ttagagatta tagttcctgc
     2041 aacaagtgct aatataggtc caggatttga ttgtcttgga attgcgttaa atatatacaa
     2101 taaattttat gttgaagaaa ttgaaagtgg tcttgaaata gaaggttgtg aagatgctta
     2161 caaaaacgaa aataacttag tttatacatc tatgaagtac ttttttgata gagttaaacc
     2221 agaaaaaata cctgctggta tcaaaataaa aatccaaagt gaagtaccta tatgtagagg
     2281 tcttggcagt agtgcctctt gtatagtagc tggagttata gctgcaaatg ctctatcagg
     2341 tgctaattta gataaaaatc aattactaaa tatagcttca gaaatagaag gtcacccaga
     2401 taatgttgct cctgctattt taggaaatat gattgtatct gttactgata atgagaacat
     2461 acattatgat attataaaaa ttcctgaaga acttaaattt tgtgcaatga tacctaattt
     2521 taagttatca acagaaaaag ctagaggtgt tttaccaaag gaaataccat attctgatgg
     2581 ggtattcaat gttagtagag ttgctttact aatatctgcc ttactaaaca aaaactttga
     2641 cctacttaaa gtagcttgtc aagacaaatt gcatcaagat tatagaggaa ctctaattga
     2701 gaactacaat gatattgttg aaaaatctga acaattaaat agtattggag tatttttaag
     2761 tggagctgga cctactataa tgtctttaat aaaagaaaat gatgatagtt ttgtggataa
     2821 tatgaaaaat tacctacaaa aactaaaatc tgattgggaa ataaaagagt tatgttgcga
     2881 ttctaatgga gctgttttaa atataatata attatcgtct tctatacaaa atataactac
     2941 tcttttatta taatttgtag tataataatt aggactaggt aataatatta agtattataa
     3001 tctagttctt taaacatgtt tacattgagg agggattatc ataaaagagt taataattag
     3061 agatttagtt gcaaaagttc ctataattca aggtggaatg ggcgttggta tatctaggtc
     3121 atcactagct ggtgcagttg ctaagcttgg tggtgttggt gtaatttctg gagttcaaat
     3181 tggatatgat gaggaagatt ttgaaactaa tactataaat gcaaatttaa gagctataaa
     3241 aaaacatatt tctaaggcca aagaaatctc aaatggtggt attattggta ttaattttat
     3301 ggtagctatg aaagaatatg aaacatacgt aaaggaagct gttaaagctg gtgtagacct
     3361 tataatatct ggagctggat tacctaataa gctaccatca ttagtaaagg gaagtaatgt
     3421 aaaaatagca cccatagttt caactgcaaa agctgctaat gtaattttaa aaatgtggga
     3481 tagaaaagag aaaactactg ctgacttaat agttgttgaa ggtccaaaag ctggtggtca
     3541 ccttggatac tctaacgagg aacttgataa catagattct atagactatg ataaggaatt
     3601 tgttgaaatt ttaaaggttg caaatactta tggagaaaaa tttggtagaa acataccagt
     3661 agttgctgct ggtggtataa cttctagtag tgatgttaaa aaatatatag atatgggtgc
     3721 aagtggtgta caagttggta caagatttgt tgctacttac gaatgtgatg cccatgagaa
     3781 ctttaaaatg gcatacataa atgctagtga agaagatgtt catatagtta aaagtcctgt
     3841 aggtcttcct ggacgcgcca taagaaacaa atttatagaa gaagttaaaa taaatcgtcc
     3901 agaaataaag aagtgctata attgtttgat tccttgtaat ccaaaagaaa ctccttactg
     3961 tatttctcaa gctctaatca atgcagttaa aggagacgtt gaaaattcac ttttattttg
     4021 tggaaatgat gcctataaaa ttgataaatt aagtactgtt gaagatgtta tcaatgaatt
     4081 gatttctgaa ttataattta aagaaaacta aattaagatt ttatcaagct aaggagttta
     4141 aatacgttat ttattaatct ttataaacat atttatgtcc ttagcttttt tgtgtcaatc
     4201 tccttttatg gaaattttaa tttaattaga gatagtatta acccttataa ctaatcttct
     4261 tttacaagta cattggttat ttcagctata tataaagtat gataatcttt ttgtggatac
     4321 catcttccat ctataccttc agcaataaaa cattggggtt caagtttctg actatacatt
     4381 tttttgcata taatagccat tttagcttcc ttaaaataag gtgtatcatt taagtgttct
     4441 atattaaatc caatctgtga tatcttatcc tcttctctac cagatacttt tccacaatat
     4501 gaaagctctt tacgaaaatc ttcatcaaaa aatgtaagag aaaatttatc tgtattatct
     4561 ataaattcct tagtataacg ctgtggtctt attactacaa aagcaacatc ttttccccac
     4621 ataactccaa gccctcccca gctagcagtc attgtattca ctttaccttc attttctgct
     4681 gttataagtg tccaatcttt acctatcaat ccaaatggat tatttttaat ctcttctgcc
     4741 aaaatttctt taaaatcact catatctcta ataacctctc attctcttat atttatattc
     4801 cttttatttt cgtgataaat tataaatgaa gtctagtttt gaaaataaaa tactttttta
     4861 aatatattat tgcaaataag taaatatata tatactctct tattttttaa tata
//
LOCUS       DAEMIK010000214         4866 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEMIK010000214 DAEMIK010000000
VERSION     DAEMIK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.217"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            303..1163
                     /locus_tag="KPK87_003590"
     CDS             303..1163
                     /locus_tag="KPK87_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2539693.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1153..3024
                     /locus_tag="KPK87_003591"
     CDS             1153..3024
                     /locus_tag="KPK87_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2539694.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIEGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3044..4498
                     /locus_tag="KPK87_003592"
     CDS             3044..4498
                     /locus_tag="KPK87_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2539695.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1873 a          507 c          896 g         1590 t
ORIGIN      
        1 tattataaat attagtgacg gtaaaatagt atcaatttag tatataaacc ttgtcagata
       61 atcgcataac agagcttgta gttatagaat aaaaaaatat ctaggaaacc gttgacagat
      121 ttatatggat agtataacat atagaatatg aaattaaaca aaaaagaatt ttaattatgg
      181 cgacgtagtc ataattttga gtatgttact gattagcagg cagaactcaa gtatatatgt
      241 gaaaaataga gggatactct atacatatat gcttgagttc tttttattga agggaggaat
      301 atatgaaaat aattcagatt ttaaataata atgtagcctt agtaaaaaga ggtaaaaatg
      361 aaattattgt atttgttaaa ggaattggct ttaaaaaaag agtgggacaa gtaataaatg
      421 aaaatgaaat agagagaaca tatatactag attcatacga tatgctagaa cactttagtt
      481 atctactaca aaactcagac tccaataata tagttcttat taatgaaata attgaatatg
      541 gtgagaaaac tttaaatata aaggcaagtg attatctaag cttaacctta ctagaccatt
      601 tagagttttt acttaaaaga tgtgaaaaga atcaatttat aaaaagtcct ttggtatgga
      661 atgttaaaag attttatcca aagcattttg aaattggaat gcatgcacta aaattaattg
      721 gtgatagtaa gggaattaat cttcctgatg atgaggcagt atcaatagca cttcattttg
      781 taaatatgga ggtaaataaa gagtctcatg atagtacaat tgtagagttg agaactttag
      841 cagatatagt ttctataata aaatatcatt ttaatgtaga gctagatgaa acttcaacaa
      901 attatatgag atttactact catctacagt actttatcca aagaattatg tcagaggaag
      961 cttcaataga tgaagataat tctatagatt tatataaaca agtaagtcaa ctttatccaa
     1021 agtcatttgg aagtgttcaa aagataagaa aatatataaa atcacaattt aatgtcgata
     1081 tatctataag tgaagaaact tatttaatgt tgcatataaa tagggtgact caaagactgg
     1141 aggcaaaatc agatgaaata taaagattta aatgaaaata taataaaact tataggggga
     1201 aaagaaaata taaagtcggt agcgcattgt gttactcgtt taagatttac tttaaatgat
     1261 aggagtatcg ctcaaacaga agaaattaaa aaattaaaag gagtaattga tgtagtttct
     1321 aatgatgttg cttatcaaat tataataggt actcatgttg tagatgtata taacgagttt
     1381 atgtctatga taggtttaag ttcaagtgaa tcaagtataa aaagtgaaag aaaaaaggga
     1441 attagaggta tcaaaggtat atttaattcc atctttgtgg tagtctcaga gactatgact
     1501 tcagtaattg aagtactact tgcagctggt atacttgcaa gtatattagc acttttaaat
     1561 atgtctggca tacttcagag tgacagtcct acatatcaaa tattagatac attaagaagt
     1621 gctacatttc acttcttacc agtattaatt gcaatagcat cagcaaaaag acttaatgta
     1681 aatccatata tagcagtagt attagcagtt acgttattat cttcatcaat agatggtgta
     1741 gaaggattaa gtttatttgg tataaattta cagtctacaa cttatgcaaa cagttttatt
     1801 ccaatacttt taggagtatg ggcaatggga attataattg aaggtttgaa aaaaatatta
     1861 cctaaagcac tacactattt tttagttcca gtattatctt taataataac tctatcagtt
     1921 acattaactg tatttggacc tattggtatg tggattggtg atggattaaa ctatgcatgt
     1981 atgtttttaa ttgatacatt gggtaattgg agtgttgttg cactgtatgc agctttacaa
     2041 ccactcttaa tagtctttgg tgcagcaaac tttactatgc ctatatcttt aaattctgtg
     2101 acaacattgg ggtatgaccc aatttttatg ggcgctgcaa caatatctga tattgcagtt
     2161 tgtggtgcaa tgtttggata tttcttaaaa actaggaata aagaacaaaa acaattattt
     2221 ggtacagtaa gttttagtgc acttatggga gttacagaac cttcagtgtt tggtgtgttt
     2281 atgaaatata gaagaccatt tgtagcagta atgataggtg gaggtatagg tggtttaata
     2341 gaaggtttag ctcaagttaa aacatatgga atggtttggg gattagctgc attaccaacg
     2401 tatcttgttg gaggagaggt atctaacttt acatttatga taataagtgt agtagtatca
     2461 tttgtagcag caacagcagc ttcctattta ctaggaatac cttcagatga aaaagaagaa
     2521 gttaagggtg ataatgaaaa agaattagaa ctaataatca acgagaacaa ttcaagaact
     2581 attagtattg gaaatgttgc aaaaggtcag gtaattgcac ttggggaagt taatgacaaa
     2641 gcattttcct ctggagcatt aggtaaaggg gttggagtta tacctagtga atcaaaatca
     2701 actgttatat ctcctgtaga tggaactata actatagtat ttccaactaa acatgcatat
     2761 ggtatagtga ctgatgaagg attagaaata ttaattcata ttgggataga tactgtaaac
     2821 ttagatggaa aattctttga aagtcttgta acacaaaatc aaagggttaa aaaaggagat
     2881 gctttagcag tatttgaatc tgataaaatt atagaggaag gctttgattc tactataata
     2941 actgtagtta caaatactag cgattattta gatgtaatct catcaaatga tgaatcagaa
     3001 gaattattaa ctgtaatagt ataaatttta ggagggaaaa ttcatgaata ttaaagatac
     3061 atttttctgg ggaggaagta ttgcagccca tcaatgtgaa ggttcatggg atagtgacaa
     3121 caaaggacct gcaattatgg attttgtaac taagggctct tatgaaatgc caagagtaat
     3181 tactgataaa atagaagaaa aattggatta tccatctcat aatggaatag atttttataa
     3241 tagatataaa gaagatatag ctttatttaa agaaatggga tttagtgcat taagaatatc
     3301 tatagattgg tcaagaattt ttccaaatgg agatgatgaa aatcctaatg aattagggat
     3361 taaatactat gaaggtctga tagatacatt aatagaaaat aatatagaac ctattgtaac
     3421 actatatcat tttgaattac ctatgaattt agtacataaa tatggttcat ggaataacag
     3481 aaaacttata gatttgtatt taaagtacag tgagactgta ataagaagat ttgatgataa
     3541 ggtaaagtac tgggttacat ttaatgagat gaaccatatt gaccctcaaa cagagcactc
     3601 ggatattttt acttatatga tagcaggtat taagtatagt gaaatagaaa ataaggttca
     3661 aacattggca aatgtagggt ataatatgac tttggcgggt gtaaaggttg caaagttggc
     3721 tagagaaatc aatcccaaca ataaagttgg atgtgtattt ggactaaatc caatttatcc
     3781 atacaattgt aatcctagta atgtgttaaa tgcattttta gataatgacc gtgattatta
     3841 tcaaattgat gcaatgtgca atgggaagtt tccacaatat aaattaaaag aatatgaaaa
     3901 aaataatata actattgaag tgactgatga agacaaaaaa gcatttaaag aaggtaagtt
     3961 agattttata ggacttaatt attatttttc aagtgtatct actccaaaag aaaatttatc
     4021 aggtgataaa tctttatttg gtgggattca aaatccatat ttggaacaaa gtaagtgggg
     4081 gtgggcaata gaccctatag gtttaagatt taccttgaac tatatttaca gaaagtatca
     4141 attacctatc ttaattacag aaaatggttt aggggcatat gataaaatcg aagaagatgg
     4201 aactataaat gatgattata gaattgaata tttaggtaag catctatctc aaatgaagtc
     4261 tgcaattgaa gaagataatg ttgaatgttt tggatatttg atgtggggac caattgattt
     4321 agtcagtgca actacaggtc agatgagtaa gagatatgga tttatctatg ttgacttaga
     4381 tgatgaaggc aaaggaagtt ttaagagata taagaaaaaa tcatttgatt ggtacaaaga
     4441 agtaataaaa tctaatggag aagttttaga tttttctacg gatatagtag tagggtagga
     4501 ataagataaa tagaagttag gaactaatgt taattagtgt ctagtaaagc atttaaaata
     4561 gagtataggg tctttaaagt aaagtgtgta ttcttcttaa aagtaatggc aactaaaatt
     4621 actataaaaa ggagaataca cattttttat gtttatactt aagtaaatat ttagacatat
     4681 tcatagtata atttactcat tatataaaaa tagtatgatt tactcactat ataactttat
     4741 aaattttctg gaaaaatgtc agagtatatt gacaaagaaa agatttaaaa ttataatcta
     4801 tattaagaat agtttgacaa tataaaaaca agtattttta taaatagtga aattattttt
     4861 attgtc
//
LOCUS       DAEMIK010000215         4856 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEMIK010000215 DAEMIK010000000
VERSION     DAEMIK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.218"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..883
                     /locus_tag="KPK87_003593"
     CDS             <1..883
                     /locus_tag="KPK87_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG2539696.1"
                     /translation="KKNLKKHLDLTTRVNLVQNLLKTKELPVKTGATLLDINRTSVYY
                     KGMPISQEELDCKSIIDRLHTDNPAWGARQLSAQLKKRGHQVGRRKTRRYMNEMGIDP
                     IYPKMNLSKRMRQAKVCPYLLRNAVIDRPNQAWSIDITYIPIKRGFLYLTAVIDWYSR
                     CIVGWEVDDTLDTRMVITALKKAFIVAKPVILNSDQGCQFTSNEYMNFLKENQIRQSM
                     DGKSRWADNIMIERWFRSFKYEEAYLTQYNNIREARKAIGKYVHTYNFERCHSALNNQ
                     TPASCYYPILLLDDHAA"
     gene            1035..1118
                     /locus_tag="KPK87_003594"
     CDS             1035..1118
                     /locus_tag="KPK87_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBG2539697.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            1243..1980
                     /gene="erm(B)"
                     /locus_tag="KPK87_003595"
     CDS             1243..1980
                     /gene="erm(B)"
                     /locus_tag="KPK87_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBG2539698.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1925..2098
                     /locus_tag="KPK87_003596"
     CDS             1925..2098
                     /locus_tag="KPK87_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBG2539699.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSGPH"
     gene            2342..2581
                     /locus_tag="KPK87_003597"
     CDS             2342..2581
                     /locus_tag="KPK87_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539700.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSLIESGKSDPTL
                     KTLERIAELTNSTLVIDLIPNELEQVELQIEEEKQ"
     gene            2976..4466
                     /locus_tag="KPK87_003598"
     CDS             2976..4466
                     /locus_tag="KPK87_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBG2539701.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKLVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALSEFFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            4581..>4856
                     /locus_tag="KPK87_003599"
     CDS             4581..>4856
                     /locus_tag="KPK87_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2539702.1"
                     /translation="MSRTRRNFSAKFKSELVIELLKGEKDLNTIATENNIQPNLLRNW
                     KKEFLDKASVVFNDTREDNLKEKLALERKEKAEYAKKVGQLTMQVDWL"
BASE COUNT         1756 a          820 c          897 g         1383 t
ORIGIN      
        1 aaaaaaaaat ctgaagaaac acttggacct gactacgaga gtaaatttag tccaaaacct
       61 tttgaagact aaagaacttc cagttaaaac aggagctaca cttcttgata tcaatcgtac
      121 cagtgtttat tacaagggca tgcccatatc tcaggaggag ttggattgca aatcgatcat
      181 agatcgatta cacacggata atccggcctg gggagcacga caactgtctg ctcaactgaa
      241 gaagcgtggg catcaggttg gtcgccggaa aacgcgccgt tatatgaatg aaatggggat
      301 tgatccaatt tatccaaaaa tgaacctttc taaacgtatg cgacaggcta aagtctgccc
      361 gtatctgcta cgtaacgccg ttatcgaccg tccaaatcag gcatggtcaa tcgacattac
      421 atacatcccc attaagcgtg gatttctgta tctgaccgct gtgattgact ggtacagccg
      481 ctgtatcgtt ggctgggaag tcgatgatac tctggatacc agaatggtca taactgcgtt
      541 aaaaaaggcg tttatagtgg caaaacctgt tatcctgaat tcagatcagg gctgtcagtt
      601 tacaagcaat gagtacatga atttcctcaa agagaaccag atccgtcaaa gcatggatgg
      661 taaaagccgg tgggctgaca atatcatgat tgaacgatgg ttccgaagct tcaagtacga
      721 ggaagcatat ctgacccaat ataacaatat cagagaagca cggaaggcaa tcggtaaata
      781 tgtgcacact tacaactttg aacgttgtca ttctgcactc aataatcaaa caccggcatc
      841 ctgctattat ccgattctgt tactggatga tcatgcagcc taagggggga ttttctcccc
      901 tacccagtta catatatcag ttcattataa aaagcttaga tttttgtctt gacaactgaa
      961 ccactatatt gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga
     1021 aggagggatt cgtcatgttg gtattccaaa tgcgtaatgt agataaaaca tctactgttt
     1081 tgaaacagac taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc
     1141 agtgactaat cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact
     1201 tctgtattta tttatagatg taatcacttc aggagtgatt acatgaacaa aaatataaaa
     1261 tattctcaaa actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat
     1321 ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa
     1381 ctggctaaaa taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta
     1441 tcgtcagaaa aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag
     1501 tttcaattcc ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc
     1561 acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt
     1621 gaagaaggat tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac
     1681 actcaagtct cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca
     1741 aaagtaaaca gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat
     1801 tggaagctat atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt
     1861 actaaaaatc agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt
     1921 acttatgagc aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa
     1981 ttctatgagt cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa
     2041 attattaggt atactactga cagcttccaa ggagctaaag cgctcgggac cccattaagt
     2101 atttttaaaa gcatggtcgc aagtttcact agcagccatg cttttattga atcattgtag
     2161 gacattttaa agataattgc ccactttaaa attaaacatg tgcttagaat cgttctaggc
     2221 aacaccaata acaatcacta atcacaagtg atttgtgatt aatcacttgt gatttacaag
     2281 gtgtaacgat ataattaaat ggaaaacgtg caaaatattt acataaacag gagggagaaa
     2341 cttggaacta gcatttagag aaagcttaaa aaagatgaga ggtaccaaat caaaagaaaa
     2401 attctcccaa gaattagaaa tgagtagatc aaattattca ttgattgaat caggaaaatc
     2461 agatccgaca ttaaaaacat tggaacgaat tgcagaattg acaaactcaa ccttagttat
     2521 tgacttaatt cctaatgaat tagaacaagt agagttgcaa atagaagaag agaagcaatg
     2581 attgtcgtta atatatttaa cgaaattgtt gcttattttt tttaaaagcg gtatactaga
     2641 tataacgaaa caacgaactg aataaagaat acaaaaaaag agccacgacc aacttatttt
     2701 ccgccaagaa aataagttgc gagccttaat tgattaccac caatcaatta aagaagtcga
     2761 gacccgaaat ttggtaaagt atttaattac tttattaatc agatacttaa atatctgtaa
     2821 acccattata tcgggttttt gagggaattt caagtcttta agaagatacc aggcaatcaa
     2881 ttaagaaaaa cttagttgat tgcctttttt gttgtgattc aactttgatc gtagcttcta
     2941 actaattaat tttcgtaaga aaggagaaca gctgaatgaa tatccctttt gttgtagaaa
     3001 ctgtgcttca tgacggcttg ttaaagtaca aatttaaaaa tagtaaaatt cgctcaatca
     3061 ctaccaagcc aggtaaaagc aaaggggcta tttttgcgta tcgctcaaaa aaaagcatga
     3121 ttggcggacg tggcgttgtt ctgacttccg aagaagcgat tcacgaaaat caagatacat
     3181 ttacgcattg gacaccaaac gtttatcgtt atggtacgta tgcagacgaa aaccgttcat
     3241 acacgaaagg acattctgaa aacaatttaa gacaaatcaa taccttcttt attgattttg
     3301 atattcacac ggcaaaagaa actatttcag caagcgatat tttaacaaca gctattgatt
     3361 taggttttat gcctacgtta attatcaaat ctgataaagg ttatcaagca tattttgttt
     3421 tagaaacgcc agtttatgtg acttcaaaat cagaatttaa atctgtcaaa gcagccaaaa
     3481 taatctcgca aaatatccga gaatattttg gaaagtcttt gccagttgat ctaacgtgca
     3541 atcattttgg gattgctcgt ataccaagaa cggacaatgt agaatttttt gatcccaatt
     3601 accgttattc tttcaaagaa tggcaagatt ggtctttcaa acaaacagat aataagggct
     3661 ttactcgttc aagtctaacg gttttaagcg gtacagaagg caaaaaacaa gtagatgaac
     3721 cctggtttaa tctcttattg cacgaaacga aattttcagg agaaaagggt ttaatagggc
     3781 gtaataacgt catgtttacc ctctctttag cctactttag ttcaggctat tcaatcgaaa
     3841 cgtgcgaata taatatgttt gagtttaata atcgattaga tcaaccctta gaagaaaaag
     3901 aagtgattaa acttgttaga agtgcctatt cagaaaacta tcaaggggct aatagggaat
     3961 acattaccat tctttgcaaa gcttgggtat caagtgattt aaccagtaaa gatttatttg
     4021 tccgtcaagg gtggtttaaa ttcaagaaaa aaagaagtga acgtcaacgt gttcatttgt
     4081 cagaatggaa agaagattta atggcttata ttagcgaaaa aagtgatgta tacaagcctt
     4141 atttggtgac gactaaaaaa gagattagag aagcgctagg cattcctgag cggacattag
     4201 ataaattgct gaaggtactg aaggcgaatc aggaaatttt ctttaagatt aaaccaggaa
     4261 gaaatggtgg cattcaactt gctagtgtta aatcattgtt gctatcgatc attaaagtaa
     4321 aaaaagaaga aaaagaaagc tatataaagg cgctgtcaga gttttttgac ttagaacata
     4381 cattcattca agagacttta aacaagctag cagaacgccc aaaaacggac acacaactcg
     4441 atttgtttag ctatgataca ggctgaaaat ggaagaagtt ttaattgatg tataatggta
     4501 tcgtaattta agacacttca agagacattt cttaatgaat ctgatatact gtaaacacta
     4561 cagaaagaag gattattatt atgtctcgaa caagaagaaa tttctctgcc aaattcaaat
     4621 cagaattagt gattgaactg ctcaaaggag aaaaagactt aaatacaatc gcaaccgaaa
     4681 acaatattca gccgaatctt ctccgcaact ggaagaagga gttcctcgat aaagcatccg
     4741 tggtttttaa tgacacacga gaggataatc tgaaagaaaa actcgcttta gaacgcaagg
     4801 aaaaagctga atatgcgaaa aaagttggcc agctcaccat gcaggtggat tggttg
//
LOCUS       DAEMIK010000216         4821 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEMIK010000216 DAEMIK010000000
VERSION     DAEMIK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.219"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            335..844
                     /locus_tag="KPK87_003600"
     CDS             335..844
                     /locus_tag="KPK87_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG2539703.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            872..1066
                     /gene="rpmI"
                     /locus_tag="KPK87_003601"
     CDS             872..1066
                     /gene="rpmI"
                     /locus_tag="KPK87_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG2539704.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1122..1478
                     /gene="rplT"
                     /locus_tag="KPK87_003602"
     CDS             1122..1478
                     /gene="rplT"
                     /locus_tag="KPK87_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG2539705.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1788..2333
                     /locus_tag="KPK87_003603"
     CDS             1788..2333
                     /locus_tag="KPK87_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539706.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2773..4698
                     /locus_tag="KPK87_003604"
     CDS             2773..4698
                     /locus_tag="KPK87_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2539707.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
BASE COUNT         2035 a          488 c          913 g         1385 t
ORIGIN      
        1 tcatgtagaa tatacatata taaaaggatt ttgggtataa atttaaaaat taatagcaaa
       61 atgagtagta tagacatata attagtagta ggaagtaatt aaatttttcc taaacaaaaa
      121 aaatagttgc aaataatttg attaagtgat atagtaaata aggtaagaat aaaacaaaga
      181 agaagtccgc ttctcacctt acgccggagg ttagtaaggt aaatataagt taaagcttaa
      241 ttttaattta agtttgttta aatttggtaa cttatattgc ggatgattac gtcatccgct
      301 ttttatttta taaaaatttt ggaggtgtcc tacaattagc aaagaattag caatcaatga
      361 acaaataaaa gataaggaaa taagagtcct ttcaccaaca ggtgaacaac ttggagtaat
      421 gcctactaaa gaagctcaag caatggctaa tagcaaaaac ttagatttgg ttcaaatctc
      481 gccgaatgca aatccaccag tatgtaagat aatggattat ggaaagttta gatatgagca
      541 agcgagaaaa gagaaagaag ctaagaaaaa acaaaaaact atagttgtaa aagaagtaag
      601 acttagacca ggtattgaac aaaatgattt aaatacgaaa gctaataatg ctataaagtt
      661 ccttaaaaaa ggggataaag tcaaagttga acttagattt agaggaagag aattaggaca
      721 taaagacata ggtaaagaag ttatgcttaa gtttttagat ataataaaag agtttggaga
      781 accaacaaaa gcccctgcat ttgagggtaa taatatggtt gtaattatag atccaaaaaa
      841 atagatgatt aactgagagg aggaatttgt catgcctaaa atgaaaactc atagaggtgc
      901 agcaaagaga ttaaagaaaa caggaactgg aaagttaaag agagctaaag cttttaaaaa
      961 acatatatta actaaaaaat ctgcaaaaac taaaatgaac ttaagaaaat caactctagt
     1021 aagtgatgga gatgctaaga gaatagcaca attattacca tactaagata cagattgtaa
     1081 ataataacta tagaagaggg atataaaagg aggtcataac gatggcaaga gttaaaaaag
     1141 ctatgaacgc tcgtaaaaag cataagaaaa tattaaaact tgcaaaagga ttcagaggat
     1201 ctagaagcaa attatataga ccagcaaata cattcgtaat gaaagcatta aaaaatgctt
     1261 atataggaag aaagttaaag aagagagact tcagaaaact ttggatacaa agaataaatg
     1321 cagctgctag aatgaatgga atatcttatt caagattaat gaatggatta aaattatctg
     1381 gtgttgaagt taacagaaaa atgttatctg aaatggctat acaagaccca gaaggatttg
     1441 caaaattagc agaagttgct aaagcaaaat tagcttagta ttgaatcatg agatgtaaag
     1501 tctttgagtt atataaataa ttcaaggact tttttatttt ttgaaaaata cttattagtg
     1561 tcatgtacaa ccaattttat aatgataaat aaaataagca atacaataga attttatgag
     1621 atttattata agaattgtta caattattta tgatagctat aaaaagtcat agtaaaaatg
     1681 atattgatgt atgttgatat atgtataaag ctttcaatca atcttgaatt aatattatca
     1741 acatgataat attatcttat taataatata aataaggggg agataatatg aatgaaaaac
     1801 taatggattg ttttgtaaac ccaattaaat gcaagttatt gcttgaagtt ttttcaaata
     1861 atcgaactac agctaaacaa ttaggagaaa aatttcaaga tatatcaaag gcaacacttt
     1921 atcgttacct taataaaatg ttgaaagatg atattataaa agtggttgaa gaaaataaaa
     1981 taaggggaac tgttgaaaaa gtatatgcta tgaagttaga tatagctgat gatgtgaaca
     2041 aaattataga tgagaactct ggagaagaat atgctcaact gtttactcaa tatatgatgg
     2101 gatttttaag agaattttat gaatacgctg aaagaaagga aaaggatatc ctaaaagatg
     2161 gttcaggatt ttctgtctgt ccagtatatg ctagtattga tgagatagtt gaagtgagta
     2221 aaaaaattgc tagtatacta gaacctctaa gaaataactc acacgaagaa ggcagaagaa
     2281 tgcattctat aggagtgata atcactccac ctaaagagga tgaagggaaa taaatctgat
     2341 ttacatgggg tagagccaat ttatacaggg aatcgtttta tgatttacat atgtatccag
     2401 agcaaagtat ctctgcttgg attgtacttc attgtatcat ttcatctatc taatgacttg
     2461 gagtggtaca atgaagtttt ttaatttttc atattaatgc ctaaagaaat gaactttaga
     2521 ccacatatgc aatcaggtta cattatacaa ttagaccaga tatacaaaat aaaaatcagt
     2581 atttttatag cagataaatt gactgttatt cttcttaaaa atcatctatt agttattgtt
     2641 agcatattga aagaaattat aggaatctat tgaataaatg aatattgccc ataatcatat
     2701 aaactgctat agtttaaaat aaagcaggac gttaaatttt atagtaatct aaaaatttaa
     2761 gagggggatt ttatgaaaat aaaatttgtt attatgccat tggtgatatt tttaatggta
     2821 ttatttgtgg ggtgtcaaag tacaaaagat acagaagtta aagcaaaaga tacagaaatt
     2881 aaaatattag gtacaggtga tttgcattca attttgacag actctatggt ttcatatgta
     2941 aatgaggaaa gggaaaagaa taaaaatctg cttatggtag atgcgggaga tttctatggt
     3001 acacaaagta gagaaatgtg ggaatggtct tctggaaaaa agttaattaa tattaaaaga
     3061 aatggtagag cagagtatga tgacataatt aagagtagta aagatgaagt acctatagtt
     3121 aaagatatgg caaagttgaa atatgatgct gtagttttgg gagataatga atttgtttca
     3181 aatgataagc aaagtcttga taaactagtt tctgatttta aaaataataa tatgccattg
     3241 gtatcagcaa atatatatga acaatctgga gaaaattatg tacaaccata tgtaatgaaa
     3301 aatattaaga cagatgaggg caatgtaaaa gttggaatac taggtcttac tataaaagaa
     3361 gtaggggaaa cccttggtct tgaggatttt gaaaaggata aaaaagctag agagcttgga
     3421 gaacaagctg attacaaagg aaaattatat gcaaatgatt tagtagaaga tgcaaagaaa
     3481 tggataaaag ttatggaaaa agaaagtcca gacataatag ttgcagttgt acactcaggg
     3541 gaagaaccta aaacagatag aaatccagga aatagaataa aagagttagc aactacagtg
     3601 gatggaatag atgcaattgt agctggtcat actcatgaaa aaatagatga acacaaatat
     3661 aaaaataaat ctggagatga agtaattgtt acacaaccag gttggcatgg agatagagta
     3721 tcagagatta atttcaaatt aaataaagag aatggaaagt gggacataaa ggaaaagtct
     3781 agtaaattaa aaataattga taaagaagta agtatagttg ctactgcaga tttacattca
     3841 catttctctg ataaattaac agttgacctt gttaatgaac gtagtaaccc aattgaacct
     3901 gttgtagtag atgcaggcga cttcttagac cctcaaacag atgaaatgac tcaatggtat
     3961 aaagagtgga aaagtataag agataataat agtgataaaa cgattagtag atgcccaatg
     4021 gtcatggata tgaatcaggg aatgtatgat gcagtagtac ttggaaatca tgaatttgta
     4081 tctgaaaaag atgaattttg gtcagatgaa cgccttttag atgctgttat agaggatttt
     4141 gaacaggtgg caatttctgt gttatcggca aatatatata ggcaatctgg taaaaattat
     4201 gttaaaccat atattataaa agatgtggag acgagtgaag gtaatgtaaa agtaggaata
     4261 ttgggactta ctataaaaga agtaggtaaa ggtcttaaaa atgtaaactt acaagagcag
     4321 tttcaatata aggataaatt gtatgcaaat gatttagtgg aagatgcaaa gaaatgggta
     4381 aaagttatga aaaagaaaaa accagatata atagttgcag ttgtacattc aggagaagaa
     4441 ccaaaaaatc caaaacatcc aggaaataga ataaaagaac tagcaactac agtagatgga
     4501 atagatgcaa ttgttgctag tcatactcat gaaaaaatag atgagcatga atataaaaat
     4561 aagtctggag aaaaagttat agtgacacaa ccaggtgagc atggtaaata ttattctaaa
     4621 attaccttta aagtaaataa agaaaagggt tcgtggacta ttaatgataa attcagtaaa
     4681 acaattgagg ttgaataact ttataaaatt tatagataaa gtaaattatc tgatattgac
     4741 atgatatata agataaagtt tctaatatta gtataatgca taatgtgaaa tattttaata
     4801 ataagagaat aaacaagaca a
//
LOCUS       DAEMIK010000217         4747 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEMIK010000217 DAEMIK010000000
VERSION     DAEMIK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.220"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            322..1239
                     /locus_tag="KPK87_003605"
     CDS             322..1239
                     /locus_tag="KPK87_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2539708.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(1368..2297)
                     /gene="pfkB"
                     /locus_tag="KPK87_003606"
     CDS             complement(1368..2297)
                     /gene="pfkB"
                     /locus_tag="KPK87_003606"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG2539709.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            2564..3289
                     /locus_tag="KPK87_003607"
     CDS             2564..3289
                     /locus_tag="KPK87_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2539710.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3372..4526
                     /gene="nagA"
                     /locus_tag="KPK87_003608"
     CDS             3372..4526
                     /gene="nagA"
                     /locus_tag="KPK87_003608"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG2539711.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
BASE COUNT         1763 a          628 c          670 g         1686 t
ORIGIN      
        1 ttttatagtt tatctttaaa aatttaataa aatcatactc tatatacttt agtattttta
       61 ttttacgata aaaacttgtt cagaccaaag tggtaattac cactaatcca aatataaatt
      121 aatttgaatt ttttgtcaat acataaaata tttatatcaa tcaattttat ggatatccta
      181 caaaattact ttatcaatat ttcttatttc gctaatttac ttatataagc tttaaattta
      241 ttttttgata caaagtttac aaatttatgt gtctgaatta taatcaattc tgtaaatata
      301 aaaaccttag gagaaagtat tatgaaaaat ataaaaatgg gaatgatagg tcttggctcc
      361 atagctcaaa aggcatattt acctatatta actaagtctg agagatttga gtttgtagga
      421 gcttttactc caaataaagt taaaagggaa aaagtctgta gtgattatag aatcatgcca
      481 ttcgattcta ttgaatccct agctaaaaaa tgtgattgta tttttctaca tagctctaca
      541 gaaacacact acgaaattat aaaaatatta ttaaatttag gtgttcatgt atatgtagac
      601 aaacctcttg cttcaacagt aagtcaagga gaagaactta tcgaattaag tacgaaaaaa
      661 aacttaaatt taatggttgg attcaaccgt agattctgtc ctatgtacaa agaaattaaa
      721 aataatgcta cagaaattgt atctatcaat atatgtaaac atggattaaa ttccttaaga
      781 aatgttaaat tcgacagtac tcttatagat gactatatac atgttattga cactgcttta
      841 tggttagcaa atgaagatgt tgaaattagt ggagaggatt tatttttaac agacaataag
      901 aatcttatat ttgtaagtca taaacttaaa ggtaaaaatt tctctataaa cacctctatg
      961 catagagatt ctggaacaaa gttagaacaa gttgaaattc tttcaaaggg taagattcaa
     1021 agagttaaga atcttaatgt actggaaata gaagaaggtg gaaacctaac tttaaagcaa
     1081 agtggtgcat gggtaaacat attaaaacaa aagggttttg aggatatatc aaatcatttt
     1141 attgattgta tagaaaataa tataaaacca gctattaatg gagaagagtg tatcaaggct
     1201 caaagattac tagagaaaat tataaattca gtaaaataga tatttattat aaaaataggt
     1261 taaaaatgaa tttttctaac ctatttttat ataagactat atcttttata ttaaaattaa
     1321 aatctattac aacaacatta aatttattat attcccatta aatgtattta acttaacttt
     1381 tttacttcaa ctaaatcttg atatttatca atttctacca aatctacaaa tccagtttcc
     1441 atatttaaag cattacttat accacaagca tttgcaaatt taagtaaatt ttctatttta
     1501 taacccctta aaatacctac actaaatcca gccactgtgc tatctccact acctacagta
     1561 tttatacact ttacaactgg tacttttaca tcatatacat tatcttcgtt taaatacacc
     1621 ataccatctt ttcctaaaga tatacataca ttctctgcac ccatttcaat aagttttttt
     1681 cctgacaaga taatttcttc tcttgattct atattcattc ctaataaatg tcttatttca
     1741 tctatatttg gctttattaa atatggtttt gattttaaag ctatttttaa tgcttctcct
     1801 gatgtatcta atataaactt tatattattt gctctacata tatttcctat cttttcataa
     1861 tagtctatcg gcatattctc acaataacta ccagaagcta ccagtatttt agtatttttt
     1921 agtacttcct ctatatcatt ttcaaatctc tctaaatcgc ttttttctac tattggtcct
     1981 ttttctaaaa attctgtact aactttattg tcctcaatta tgtttaagca atttctagtt
     2041 tcctgctcta tttttgtaaa tttattttgt atatctagtt ttctaagttc attttttata
     2101 aattctccat taaatccgcc tagaaatccc atagctaaag gattttcttt taaaatttta
     2161 cataccttag ctacgtttaa tcctttacct ccagcagttg cattcttact tactaccctt
     2221 tgcacctcac ctatgtttat attattcact ctatacattc tatctataga tgggttgaat
     2281 gttattactg taatcatact agattctcct cattattaat ttttactttt ctaaacaata
     2341 aattaatctg taaaataaaa aatacctcat ataatatatc caaagtggtt attaccaatt
     2401 tgaatataaa ttattttact tgttttgtca atgtaccata attttataat aaattaatag
     2461 ccttacttat tgaattttga ttaaacttta ttcattgaca taaataattc tatattctat
     2521 aatcaaattg tggtaataac cacttagaaa ggactatatt tttatgataa aaagaaatga
     2581 taaaagacca atatatgacc agcttgttga gatattaaga actaagattg aaaatgagat
     2641 ggaaccaaac gatagaatgc tttccgaaag aaaaatttgt gatgaatatg gagtaagtag
     2701 aaccacagtt agactagcta tggctgagtt agagcatatg ggatatatat ataaaagaca
     2761 tggaaaagga acttttgttg ctgcactaag tcaaaattct caaaacttaa tggaaagtta
     2821 tagctttaca gattatatga gaacacaagg aaaaaagcca ggtacaaagg tattatcatt
     2881 tgaggtagta gaaagtacaa agtatttctc tgaaaaatta ggtataactc ctggtgaaaa
     2941 aatgataaaa ataatgcgtt taagacttgc tgatgacctt cctatgatgc ttgaaagaac
     3001 atatcttcct atgaaagaat ttataggatt gacaaaagaa aaaatcgaac aaaaaccttt
     3061 atatgaaata tttagagaag attatggcga gattattaaa gttgctgatg aagaattttc
     3121 agctggaata atctctgata aggaagctaa gttattggaa gtaccaggag attcaccttg
     3181 tctgaaatta cttcgtacca catataatga tgacaatata gttattgaat ttactttgag
     3241 tgtcgctaga agtgataaat ttgtatatag agtaagacat gtaagatagt aagttaaata
     3301 actttcaaga aaacagaatg atattataag cgtcaatgcc acaatgatag tataattata
     3361 aggaggttat tttgaaaagt tttatttatg cagataaatt ttttatgaaa catggaatca
     3421 aagaaaaagg ttaccttagt ataattaatg gcaaattcgg tgaatttcaa caagaaagac
     3481 ctaatattac tgataatgtt atagattttt caggtaaata tattgctcct ggcttagttg
     3541 atactcacat tcacggtctt ttaggtgcag atgtcatgga caatacattt gaagcaataa
     3601 acacaatttc aaaaggctta ttaaaatatg gagtaacatc tttcctacct acaactttaa
     3661 ctgattctgt agatacacta agtgattctg tagaaaatat aagcaatagc ttcaatcgag
     3721 ttgagggtgc aaaaatacaa ggtatttatt tagaaggtcc attttttaca gaaaaatata
     3781 agggcgctca aaatgaaaaa tattttaaag accctgacat caatatatta aaattatggc
     3841 aaaaaaaatc gaaaaattta ataagaaaaa tagcaattgc tccagaacgt aaaggttcaa
     3901 tagaattaac aagatatgca actaaaaata atatatcagt ttctcttggt catagttcag
     3961 ctacatttga gcaagttaaa caagtcgtta attcaggtgc taaagtgttt gtacatacat
     4021 ataatgggat gagtccttta aatcatagag aacctggaat ggttggggct gctatggtac
     4081 taaaagatac ttatgctgaa ttaatttgtg atggtcatca tgtaagccca atagctgtta
     4141 aaataatgat ggatgctaag tctagtaata acatagcttt aattacagat tgtatgagag
     4201 ctggtgctat ggaggatgga aaatatactc ttggtaagtt taatgtaaat gtaaaaaata
     4261 gtgttgcaag attaaatagt gggagtttag ctggaagtgt attaactatg gacaaggctg
     4321 tgagaaatat agttgattgg aacatatcaa ctttagaaga tgcaataaaa atggctacat
     4381 atgtccctgc tttaagttgt aatattgata atgtctgtgg ctctatatat caaggtatgg
     4441 atgcagactt tatagtaaca gataatgact ttaatataca cgaaacttat ataaatggta
     4501 tctgtaagta caaagttaat aaataggttc aatataaggt tatggaggct gtctaaaaag
     4561 agtctggaaa tatttaaagt tatttctcca aaatctgatt taacactttt atttccatat
     4621 cgttaataaa ctattgcgat agaggttatt ttaaacaaat atatattata agtatttttg
     4681 tgataatatt ttcgataata tgtacattta aagtaacttt tctattatta tttgtaagtt
     4741 tttatct
//
LOCUS       DAEMIK010000218         4687 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEMIK010000218 DAEMIK010000000
VERSION     DAEMIK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.221"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..275
                     /locus_tag="KPK87_003609"
     CDS             <1..275
                     /locus_tag="KPK87_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2539712.1"
                     /translation="ACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            287..427
                     /locus_tag="KPK87_003610"
     CDS             287..427
                     /locus_tag="KPK87_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG2539713.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            486..1067
                     /locus_tag="KPK87_003611"
     CDS             486..1067
                     /locus_tag="KPK87_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG2539714.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            1324..1698
                     /locus_tag="KPK87_003612"
     CDS             1324..1698
                     /locus_tag="KPK87_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539715.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            1781..3490
                     /locus_tag="KPK87_003613"
     CDS             1781..3490
                     /locus_tag="KPK87_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539716.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            3505..4377
                     /locus_tag="KPK87_003614"
     CDS             3505..4377
                     /locus_tag="KPK87_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2539717.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            4378..>4687
                     /locus_tag="KPK87_003615"
     CDS             4378..>4687
                     /locus_tag="KPK87_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539718.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     Q"
BASE COUNT         1938 a          423 c          722 g         1604 t
ORIGIN      
        1 aagcatgtat ggatataaat aatgagatag aaaaaagaat agaagttaaa gctacatata
       61 taaataaatt tgcaataata aaatttataa atactaaggt aaatgatatt aaacttattg
      121 ataaacgtat tcaaactagt aaggatgatg ataagataca tggaatagga cttgctagta
      181 ttaaatatat agtaaataaa tatggtggtg aaacaatagt taattactca gataatgagt
      241 ttatattaaa aataatgata ccaataaaaa gttaggagag tacaaaatga aaaaaatagc
      301 gttaaactta cttaaaaaca tctcagcatt aagttttggg atagctgtat tatcagcaaa
      361 cagtgcaagt tcttgggtag cacatcaggc aaaagagcca caagctttac aaaaactaaa
      421 aaaataacat taataaaagg acctcttctt attaaaggag aggttttaca tgttgaggag
      481 gaaatatgtt taaaagatta tcatataaat ttgccaatat attagttaat aatgagattg
      541 tagaaagtga agattttgaa atatatagat atggatttga aactttgatt tatttcatag
      601 ttaatatttt ggtagcatta tttataggaa ttatttttga tagatttata catacagtta
      661 tatttttaag ttgttattgc actttaagac agttcacagg aggttatcat gctaggaatt
      721 ataaagagtg tacattaaca tttgcagtta tttatttgat aactattttt tcagctaata
      781 atatagatat aaataaatat aagtatttat tagtattatt aatgattatt agtatactaa
      841 caatttacaa gttagctcct ttagaacata gaaacaaacc tcttagtgaa agtgagaaaa
      901 agcattatag aaagactgtg caaaaaatac tattcgtaat aatatgttta attattttat
      961 gtaaaatact taatattttt cagcaatatg tgatttatgc aataatttct atatattgga
     1021 ttgctatatt aatatatatt ggaatgaagg ttaatagtga tcagtaagat tataaaacat
     1081 tttctaaatt aattaacttc actaattaat attaacttta aaaaaataat gtcaaggtaa
     1141 gtatttgaac ttattaaata tagttgaatc tattgtgggt tgtatcaaag gacaaataga
     1201 ttcacttaat aaccagtttg taacagagta ttgaattgta aaagttaata cgatagtatg
     1261 aaaatgtaat ttgaaattac atttttataa aaattatatt aactaagggg atggatacat
     1321 acaatgaatt tgaagaaaaa aattaaaaag ttaggagtta catttgcaat aggaactata
     1381 attgtatcaa gtttagggac atctgtattt gcagatgagg ttagcacata tgctttaata
     1441 tctaaagctt tttcaggtaa aaacaatatt atatctagtt cagtatacac ttctggaagt
     1501 agaaccttta aaacgacaga ttctcaaggg ggattttctt gtaggctaac agaaagattc
     1561 tcaggctcta gcccagatag aattgtaaaa agacatgaag caaatgcttc ttcaaaagtt
     1621 agtttatcag gaggaacata ctatataagg tatgaaggtt caacgaataa tgctaaaaca
     1681 tatgcaactt taaattagta ttaaaagcat cctataatta tttttatagg atgtttttgt
     1741 aattgttata atcaaaagac tggctaggag gggcataaat atgagtagta ttaaggttat
     1801 atcagacttt ataaccaaaa taattagaaa aaatatttta tttgcaataa caattattat
     1861 tataaattac catctcatac aacgatggac acatatatcc aatagaggaa tttcatttaa
     1921 tgattactta tccttgagtg tatatacttg tatgacaatt agtattttaa ccatgttaaa
     1981 tgtaattttt tatatgagaa aaagttatga tactgtaaaa atattatgta gaaataattt
     2041 tgattatttt ttaggtattt ctgttgctgt aactagaaac aactttatat tttgttgcat
     2101 accaattata tatgctatag catactcaag tttaataaga acattgtcaa ttgtaacgat
     2161 taaatcaact atatttatat tgatatttga atggttttta ccacttacta ttttatctat
     2221 agtgatatca tttatttcag ttattataaa taatttaata ctaaagatta ccttatcatc
     2281 agtattttta tatttaacat caactaagat gttggataga gttattgata ttaaagaacc
     2341 attttttaaa gcattattta aatcacttaa catatttgaa gatcaatcat atgcttacta
     2401 ttctgatttt gtagggaaag ttaataattc agcatatgtt atagataaaa ttatacctac
     2461 actatttgca attactttaa taatagtagc atatctgata ttaaatgata ttagaaagaa
     2521 acagattaaa tcattaatag ttatagtagt gtacagtatt ttatttattg gattaaatta
     2581 tatatcaata gatatgtcaa catatagaga tgaggagcgt agttatggag agtatttaaa
     2641 gaaatctaat attagtgata ttaaaataaa atcgcatgat atgaagattg actttaaaag
     2701 caagtcaaat ataaaagata gaattgtttt attaaacaca tcaaatgaag agttaacaaa
     2761 tattaatatg attttagatg aaatatttga tataaaatat ataaaagtaa attcggaaaa
     2821 tataaaattt aacaaaaaaa aggatttaat tgaagttaat ttaaaggaaa gtttagagcc
     2881 taatgacgag gtgacattag atatagagta tgaaggctat attaatgtgt tgacatcttg
     2941 gaatcaaaat aaatatatag ctactaaaga tgatattatg cttccaccag gttctttagc
     3001 gtggtaccca aaagtaaatc aatcaagttg tatggcttat aaaataaatt taagttctaa
     3061 aaataatgtc tattcaaatt tacctttact gaataagact agtaattttt taaaaaaaga
     3121 ttatatattt gaaggaaagt caaatgatat tgcaatatat gcaggtgagt ttaaagaaga
     3181 ggtaaaagat gatataaaaa taatctatcc atcagatagt aatgtaaata tggatataga
     3241 ggaaaaaatt aaatttttaa ttgaaacaag aaatgaaata ttaggtgata gcaaagcatc
     3301 tgtagatgct aaaaaatatg tagatgaagt agataatgtt atggaatcaa taaaaagaaa
     3361 aaaaattaat atgatattag ttgctgactt acctagtaat gaatttttat atgaagactc
     3421 aaagggagag aaaattagca taactattta tcattggctt tcaggaaata tccttataat
     3481 agataaataa taaaggaggt taatatgata aacaagttag aaataagaaa tctaaatttt
     3541 aattacaaaa ataaaaaagc tttggataat ataaatttaa ccctaataga tggagtagtt
     3601 gcattattag gtcctaatgg tgctggaaaa tcaacattaa tgaggttatt agtgactctt
     3661 tatgaaactt caattggaga gatagaatta aacaatataa agtatagcaa aaacaatgag
     3721 aaaatcaagg caaatgtagg atatgtacct caagattttg atatgtataa taatataaat
     3781 gggcaagaat acttagaatt tgtagctaaa atgagaggtg tatctaagaa tgatttaaag
     3841 aaacatatac aaaaagtagt atcaaaagtt aatctggaca agtttataaa taaaaaaata
     3901 ggtacatact caggtggtgt aaagagacga ctaggtatag cacaggcttt aataggtgat
     3961 tctaagttaa tagttatgga cgaacctaca gttggtcttg atccagagca aaggaatgaa
     4021 tttagaagat tgttaccaga aataagtaaa aattctatag tattaatttc aactcacata
     4081 gtagaagaca tacaatttaa ctgtgacaaa ttaattatat taaatcaagg caaaatatta
     4141 tatgatggta caattaataa gtttattgat atggttgagg tgtattcggt agtagtttct
     4201 aatgatgaat ttgaatattt agaacgtaat attgatatta ttgattatag gagagttaaa
     4261 gatggtgtgc aagtaaagta tataggtaaa aatacagaga aacttaaaga tagcttaagg
     4321 atagatacaa ctttacaaga tgcttatata aactttttac ataaggagtt aaaataaatg
     4381 tgtatattgg agttaataaa atttgagctt aaaaaaatag agcttaaaca tgtgttaata
     4441 tttgtaatta tttctatatt atgcatattt attacaggca tattagatta taatgagtta
     4501 atattttata atgatgtacc tataatggat acatattcta tagcaggatg gttaggaatt
     4561 ataaagatat cagatgtttt tataccaata atgtcagcaa tcacaataat ttatgtgttt
     4621 tcaaaagatt attcgggtaa tataaatgag atacttactt tatataataa aaaaaaatat
     4681 aatcaat
//
LOCUS       DAEMIK010000219         4681 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEMIK010000219 DAEMIK010000000
VERSION     DAEMIK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.222"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..1388)
                     /locus_tag="KPK87_003616"
     CDS             complement(204..1388)
                     /locus_tag="KPK87_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2539719.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KPK87_003617"
     CDS             1761..4112
                     /locus_tag="KPK87_003617"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG2539720.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KPK87_003618"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KPK87_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG2539721.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         1808 a          550 c          762 g         1561 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttactatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggaa ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat ttgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagacg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg tcctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatat ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 a
//
LOCUS       DAEMIK010000220         4631 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEMIK010000220 DAEMIK010000000
VERSION     DAEMIK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.223"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..694)
                     /locus_tag="KPK87_003619"
     CDS             complement(<1..694)
                     /locus_tag="KPK87_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2539722.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISS"
     gene            968..1642
                     /gene="fsa"
                     /locus_tag="KPK87_003620"
     CDS             968..1642
                     /gene="fsa"
                     /locus_tag="KPK87_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG2539723.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKERKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHRVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1764..3158)
                     /locus_tag="KPK87_003621"
     CDS             complement(1764..3158)
                     /locus_tag="KPK87_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG2539724.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3236..4582)
                     /locus_tag="KPK87_003622"
     CDS             complement(3236..4582)
                     /locus_tag="KPK87_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2539725.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKRHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVTQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         1789 a          659 c          610 g         1573 t
ORIGIN      
        1 tagaactaat atcttctaaa tgagcaaact tgaaatttcc atcaaattta aactttttat
       61 cttccataac tataaatact tttttacttg aatttattat agccttcttc gtaagaccat
      121 cttcaatttc aaaagttgta agtgaagagt tgaatacatc tactccacaa cttcccataa
      181 aagtcttatc aaacttatat ttcgatataa actctatagt tggagtacca acaaaaccgt
      241 ctaaactgag atttaataaa cctccagtgg aaattatatt taaattattt ggttttgaag
      301 taactacatt tagtatatcc agcatatttg taacaagtgt tactttttta ttactttcag
      361 ctaacaactt cgcaagcaag atgttaattg tagagatatc taaaaatata gtttctctat
      421 cttcaattaa attaaatgct ttttctgcta ttattttctt tgttggaata ttaatttctt
      481 ttctgtcttt tgtatcctga ttctctagtg tttctctagc tagtacagct cctccataaa
      541 ttcttcttat tgaagaaatc ttttctatat tttttaaatc ttttcttata caatcttctg
      601 taacattaaa tttagaactg agctctttga cagtaactct accttctttt tttaggatat
      661 ttaatatagc ttggattctt tcttctgcaa acattttcta cctcttttcg caatatagta
      721 ttatagtaaa tattataaga taaaatacaa tatatcaact attttaacat gaaatacaaa
      781 attaatttaa tataaaaagt atgttttttc taactatata tttagaattt agtaaataaa
      841 aaaattataa aactaagaag gaaaagtaaa acaataataa aaaataaaaa acaataagca
      901 aaatgttgaa aataatacca aatcaatata taataagaat aaagaaagat taaaggggga
      961 tattttaatg caactaatct tagatacagg taatgtggag gagattaagg agttatgtac
     1021 ttgtttacca atagatggtg taactacaaa tccaagtatt gtttcaaaag aaagaaagaa
     1081 ttttaagcaa cttataaatg aaattggtga gattataggt gaggatatgc ctatacatgc
     1141 tcaagtgtta agtacaaagt atgaagaaat attagaagaa gcattatata taagtagttt
     1201 gagaaaaaat atatatgtaa aaatacctgt aacacaagat ggtttaagag ctataaaaga
     1261 ccttcacaga aaaggtgtaa aaataactgc tactgcaata ttcactgctc atcaaggttt
     1321 tttagctgca aaggctggag caaactatat tgcaccttat gtaaataggc ttgataatat
     1381 atctggtgat ggagtagcta tggtttcaga acttataaaa ataatagata cttataaaat
     1441 ggatacaaaa gtacttgcag ccagttttaa aaatgcacaa caagttatag aattaatgca
     1501 acatagggtg catagtgtta cagtaccata tgatatttgt aaatctatga tgaatcatcc
     1561 attaactgat tggagtgtag ataagtttat agaggattgg gagaatactt ttggaaaagg
     1621 aagtaaaaca aataatattt agtattatgg taaaagagta gttttgtcca atttcacaag
     1681 aacttatata aaaagatgaa aatatgaatt ttctttttaa taatttcgtt attatcttaa
     1741 gttaattcat attttcatct tttttagatt tctagtccta taaatttcca ccaagggaat
     1801 gttatacata ttattataaa gtaggttaaa ggaatcaaag ttaaaccgta tttgaaaata
     1861 accttgctat tatacagatt ttcaccatga ccaaccatta ttgtagcatg atgaaatggt
     1921 aatatgtact gtatgcttac tataatgtag acaaatagac ataaagtaac actattttcc
     1981 tttagtatat gtaaacttcc aagtaacggt attactacag atgatgtagt gacagcactt
     2041 ccaagacata tattcaagat tattgtgaga gttatcaaaa acaatattga aataatttct
     2101 gaagagtttg gtataaactt tatcaaataa tttcccatta tatctacaac tccagagtac
     2161 tttaacactc ctccaattga aaatgctgct ataaaaaaaa taaggacctt ccaatttatt
     2221 tcttttaaag tactaaatcc aactattttt cttagtaata aaagaagtac acaaattgac
     2281 attataatta atgtgtttat gttatgtatg aactgagtta aaaacataaa aaacattatt
     2341 cctaggcata aaaatacgtg tttttgctct ttgtttagca aattaaattc tttttttaaa
     2401 ttagaatatg aaaaatgaag tttattaccc tcaagatttt cattagaaat aaattttatt
     2461 gaatgcaatt cttttttaaa gacaaagata aataaaaaat aagttataaa acaagtaatt
     2521 atagttggaa cagacatata aaaagcccat tgaccccaat ttatattaca atttccaagt
     2581 tctagtacaa cataatttag aagagtgtct ccatttacaa aaaacattga tgtaaaggta
     2641 gatgctgtaa atatactaaa caaaagtata gattttgaat cttcagttat ctgttgttct
     2701 tttaaaaact ccttgtaaaa agcagataca agaataaccc ttggaaatgg ttgtgggata
     2761 aaaaatatta aaagtatacc taaaatatac gataaaagaa tcattttttt aggggtattt
     2821 acagtattta aaattaactt ctcagaaata atttttgcga cacctgtttt tgtaactgca
     2881 ttagtaatta tgtatgcaag aattattatg taaaaatttt ctgtaaataa aaactgtaaa
     2941 ataaccttta ttggtgcaat gttaaatata aacgctagaa ttacaaaaaa caatgcaaca
     3001 aaagtttttt caactgagtc tacagcccat atcaagatag ttaaaaaaga taagcatata
     3061 gaaaatttta tgttgaaact gtaattaaga gagaagtaga gaattatggt agctataaca
     3121 gtcatataaa attgttttgg cttaagaata atacccattt ttatattcac ctccataatt
     3181 tattatatca taatggaggt gaatacaatg aatgtgaata cattttataa aaagattatt
     3241 cattgattct atgatttaca atagctttag ttttccaatg tcctttctta tagtagataa
     3301 ctgtaacaat aagacctaaa gtccaaccaa aaggaatacc catccaaatt ccgtctgttc
     3361 caaaaaattt agaaagcata taagaagctg gaagtcttcc aagccataaa gatactattg
     3421 agcttataag tgcaaacatg gaatcaccag cacctttcat tgcacttgaa agtacaaaag
     3481 aagttccaat aaataagaag aatggtccta ttataaataa gtaagaagag ccaattttaa
     3541 tgacatcttt atcagtattg aatagagcaa ttagattttc cctaaatagc aaaattaaag
     3601 caataactgt aactgctaaa actaaggtca tttttataga ttctcgaatg ccttttttaa
     3661 ctctttcatt ttctccagca cctatgtttt gagtaacaaa agtagaaaca gcagaaccaa
     3721 gattcataat aggtaagctt ataaaatttt ctatcctaag accagcacca aaagctgcca
     3781 tagcagatgt accataacta ttaactaaaa attgaagagc catatttcca attgaaaaca
     3841 acatttgttg aactccactt ggaagtccta atctaagtcc atctttaaat attttgttat
     3901 catatacaat tcctttgatt ttaaatttaa gcacttcatg tcttttattt aaataaatta
     3961 tacttattat aaatgatact gcctgagcca ttatagtggc aagtgcaacg ccctcaacac
     4021 ccattcttag cactacaata aaagttaagt ctaaaactat atttagaatt gtggctatta
     4081 tcagaaaata aagtggagtt actgaatctc caagtcctct aagtattgca cttactgaat
     4141 tatatccagc agaaaataat attccactaa atattatagt aagatacaat tttgattgag
     4201 caaaaacgga ttctggtgta ttcataagct taagcattgg gccaccaaat actattccaa
     4261 gaatacttat taatatagaa ccaataaata aaagtatata tgtagtatca atagtagctt
     4321 ttaccttttt taaatctcct gagccataaa attgtgacac taaaatagaa aatccaaggc
     4381 taacacccat aagcgctgcc accaataaaa acattatagg gttagctcca gagactgccg
     4441 ccattgcttc ttttccaata aatctaccaa ctattatact gtcagctgta ttgtatagtt
     4501 gttgaaataa actaccaata agcataggca tggcaaaaaa gaatatggac tttccctcat
     4561 gacctgtagt taaatctttc accttattcc tccaaataaa aaaattatta atttattata
     4621 gtataatctt a
//
LOCUS       DAEMIK010000221         4586 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEMIK010000221 DAEMIK010000000
VERSION     DAEMIK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.224"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    355..593
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            657..2576
                     /gene="thrS"
                     /locus_tag="KPK87_003623"
     CDS             657..2576
                     /gene="thrS"
                     /locus_tag="KPK87_003623"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG2539726.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3344..4435
                     /locus_tag="KPK87_003624"
     CDS             3344..4435
                     /locus_tag="KPK87_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2539727.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1813 a          457 c          831 g         1485 t
ORIGIN      
        1 gtgttactta ttaatagtat ttaagtattt actgtactac ttattaacta tattaagtat
       61 tgattgtatt taagtataca aaacctaaaa agagtacaaa aattagaagt tagttttaat
      121 aaaaattagt tatagtattt atctagtcat atctatcatg catgtggata cgttttaaaa
      181 attttaaatt tatgaaaata ttataatatc actataaatc aagagattaa tttaatataa
      241 aaatataaga atattggttt acagctgatt gagatgaaaa ataatgggta attaatatat
      301 tacattaatg ttgattgatg tataactatc tgttataatg aaaatatatt aaaaatcata
      361 gtgttgaaga ggaagagtag agtagaaacc attctcagag aatttgggtc tatggctgag
      421 agcccatgta ataggtctat ttgaaaacta ccttggagct gagtttatta gcagtatttg
      481 tatttgtact atacgtgaaa gtgctcgggt gacgacctta tagtcatcaa gagggttgta
      541 tatacttgta tacgccaact tgggtggaac cgcgaattaa tatcgtccca aacatatttg
      601 gggcgatttt ttattacaaa aaatctaaaa attatttcaa taaggaggaa aaaacaatga
      661 ttaaagttgc tttaaaagat ggctcaataa aagaatttga aaatgctatt tctgttatgg
      721 atgtagctaa atccattagt gaaggattag ctagaaatgt ggtagcagcg tctgtgaacg
      781 gagaggttgt aggtctggac cacattatag atactgattg tgacttgaat ttatttaagt
      841 ttgaagataa agaaggtaag gaagttttta gacatacatc agcacatata ttagctcaag
      901 ctataaaaag actttaccca gaagcaaagc ttgctatagg accaagtata gaaaatggat
      961 tctactatga tatagattta gaccatagat tagttccaga ggacctagag aaaatagaag
     1021 ctgaaatgaa aaaaatagct aaagaagatt taaaaataga aagatttgaa cttcctagaa
     1081 atgaagcttt agagcttatg aaagaacaag gagaagacta taaggtagaa cttatatctg
     1141 atttacctga aagtgaaata atatctttct acaagcaagg tgattttact gatttatgta
     1201 gaggaccaca tttaccatct actaaaaagg taaaagctgt taaattacaa agtgtagcag
     1261 gtgcatattg gcgtggtgac gaaaataata agatgcttca aaggatatat ggaacttctt
     1321 ttgagaaaaa caaagactta gaagagtact tacatttact agaagaagct aaaaagagag
     1381 accacagaaa attaggtaaa gaattaggat tatttatgat acctgaagaa ggtccaggat
     1441 tccctatgtt tttaccaaaa ggtatggaac ttaaaaatga attattaaaa ttctggagag
     1501 aaatacacag aaaagctgga tatattgaga tagaatctcc aatcatatta aatagaaaat
     1561 tatgggagac ttcaggacac tggtatcatt acaaagaaaa tatgtataca gttaaaatag
     1621 atgatgagga ttatgctata aaacctatga actgtcctgg tggattaatt tattataact
     1681 ctcaactaca ttcatataga gatttcccaa tgagagttgc agagcttggt agagttcata
     1741 gacatgagtt atctggtgca ttacaaggat taatgagagt tagagcattt actcaagacg
     1801 attcacatat attcatgtta ccagagcaaa ttaaggacga aataaaagga gtagctaact
     1861 taatagatgg tatatacaaa acttttggat ttgaatataa tttagaatta tctacaagac
     1921 cagagaactc tatgggaagc gacgaagagt gggaagcagc agaaaatgga ttaagagaag
     1981 ctttagaaga gttaggtcta ccatatacta ttaatgaggg tgatggagca ttctatggtc
     2041 ctaagataga cttccatcta aaagattgtt taggaagaac ttggcaatgt ggtactatcc
     2101 aattagatat gcaacttcca agacaatttg ataatactta tattggtcaa gatggtgaaa
     2161 agcatagacc agttatgatt catagggttg cttttggtag tatagaaaga tttataggta
     2221 tattaataga gcattatgct ggtaaattcc cagtttggtt atctccaact caagttaaga
     2281 tacttcctat atctgataaa tttatggatt atgcaaatga agttaagaaa gagttatttg
     2341 ataagggtat aagagttgaa ttagatgata gagctgaaaa aattggattt aagataagag
     2401 aagctcagct tgaaaaagtg ccatacatgc taatagtagg tgaaaaagaa gtagctgata
     2461 ataatgtttc tgttagatca agagataaag gtgaaatagg tagtattaaa ttagatgaat
     2521 ttatagctag tatttcaaaa gaaattgaat caagagaaag tattattcaa gactaatata
     2581 tattatatta gtagctaaga tattgttata taagataagt ttaatcaata tgttgatatg
     2641 ttgatgcata gtgattaaat ttatgaaaag atgagtataa ttagaggtac agttaatcat
     2701 atttttatga ttaatgtacc tctagttttt tgtatggtag atttattaat aaatatatca
     2761 tagaagtgtt ttgaacatgg aaatatttat gtgcattctt taaataaaag tttatttttt
     2821 gagtgataat taagtgcttg ttggtaaggt tatggctgta aaaatcataa ctttattttc
     2881 atatttgttc cattttcata aaaattatta tccctttaag aaaatttctt aatcctcttg
     2941 aaatatttcc tttttagtct aaagttaaat ttctttcttc aaaatgaata tttaaatgaa
     3001 ctaaaattta agtacatttt ctaatcttat gagtctattt ttcatagata attgccctat
     3061 aatagaagaa tctatgttgg agctatgtgg tgacaataac agcattgtca gtgattaatt
     3121 aatgctttat aaatgattaa ttagtgtttt atgttatttt tggacttacc ctctatacat
     3181 agattaattt atttaacttt agatatagag agaatgtcaa gtacatacac agacattaga
     3241 gtttaagaaa gtgtttatgg agcaagaact tttaaaagag gtagtgactt agtttaattt
     3301 caagaaatga taaatacaaa agaggaaaaa gagaggtgtt gaaatgatag aaaaagtaga
     3361 agaaaaatct aagattatta aaacatcaaa agaatataag cgaactataa ttgaagaaaa
     3421 agataaagaa tggaccaaaa aattaaatga tatacttagt agaagtagtt tttctaatga
     3481 tgaattagat aaaatcaagg cgttaattcc aaaagagatt ttgactaatg aaaatgttgg
     3541 agaagttgtg actgaagatg gatatgctaa gcctgagtat gatgaggtat ttaaatcatt
     3601 gttcacttta aataatgaat atgatttact tgtaaatttt ataaatgata tcctaaaaga
     3661 tgctccatat gctaacagga atatcaaacc gttcactcaa ataaagagaa taattagagt
     3721 tgaaactgac ccaacaatta attacattgg agagaaaaga cctagacttg atattttggc
     3781 tgaagatgaa gaaaataatc atataaatat tgagatgcaa agagcttttg agaacgatta
     3841 tttggaaaga gctgaatatt atcttagtcg tgtacatggt agaaagttag aagaaggtaa
     3901 agagtataaa gaaattggaa aaactattgg aatacatata ctaaatcatg taaaatataa
     3961 tcacatagaa gattatgtaa attgcctgag attaacaatg gacgggcatc cagatattta
     4021 ttctagtaaa actgcattat attttataga gcttccaaaa attcataaat ctgactatat
     4081 agtaaataga attatgctat ggggaaaact tattagtaat ccttctagct tagatgtaag
     4141 agttatagct aagaatgatt ctattattaa aaaagcactt gatagattaa aagaattagg
     4201 tagcaatgaa gaatatttat taaatctgaa aagaggtgct tatatcatga ataaaagtag
     4261 aaattttaaa gaagagattg aaagagagac agcaattaga gttgcaaaag aaaaagatta
     4321 taaaataatt ataatgttga aaaaaaatgg ttttaaatta gatgacatat taaataaatt
     4381 taatgatttt gacttaagtg aagatgagat aaaagaagta tatgaaaata attgatgata
     4441 aaaagtgaac ttatataagt ttgctgaatc ggagtctgga aatatttatg tgtatttttt
     4501 aatgaataaa aatttatttt aaaaacaata atttagaggt acagttaatt atatttttat
     4561 gatactgtac ttctagtttt ttctat
//
LOCUS       DAEMIK010000222         4559 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEMIK010000222 DAEMIK010000000
VERSION     DAEMIK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.225"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            141..1109
                     /locus_tag="KPK87_003625"
     CDS             141..1109
                     /locus_tag="KPK87_003625"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG2539728.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1226..2356)
                     /locus_tag="KPK87_003626"
     CDS             complement(1226..2356)
                     /locus_tag="KPK87_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539729.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2509..3462)
                     /gene="msrA"
                     /locus_tag="KPK87_003627"
     CDS             complement(2509..3462)
                     /gene="msrA"
                     /locus_tag="KPK87_003627"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG2539730.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(3586..4491)
                     /locus_tag="KPK87_003628"
     CDS             complement(3586..4491)
                     /locus_tag="KPK87_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2539731.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
BASE COUNT         1517 a          750 c          528 g         1764 t
ORIGIN      
        1 cattgctttt ttaaaaattt tagtataaaa tcatattaaa taaattttct gaaataatag
       61 aatattaaaa tatccaaaaa cagcttttaa taacatctat aattttcaat gtatcgttat
      121 tttaggggga gtgatttaaa atggcaataa agccaagaaa aatatctatt gtaggttcag
      181 ggcatgttgg ttctcattgt ggatttagtt taataactca aggagtttgt gatgaactct
      241 ttatgattga tatagatgaa tctaaatcaa aagctcaagc tttagattta gctgatgcag
      301 tttcatatct gccacataaa gtacatatag aaaaaggaac tttttctgac tgtaaagatt
      361 ctgacatagt agtgataagt gttgcagact cttcagaagg gcctttacgt agacaaaaca
      421 caactagatt agatttacta agaccaacta ttggaatgat aaaatctata gtaaaaccca
      481 tagttgattc tggctttgat ggtatatttg tagtcatatc taatcctgtt gatgtagtta
      541 caaattatat ttgggaaaaa tctggctttc ctaaaaacaa agtcattgga actggaacag
      601 ctttagattc aacaagactt agaaggattt tatctgaaga aactggaatc gctcaacaat
      661 caattcaagc ttattctatg ggtgaacatg gagattctca aatggttcct tggtctcatg
      721 tttcaattgg tggtaaacca atcttagaca tgataaaaga caacccaagt acttatagca
      781 atttagactt accatcaata gttgaaaaaa ataaaaaaac aggaataagt ataataaatg
      841 gtaaagattg tacagaattt ggtataggta ctgctttagt agaaattgta aaagctatac
      901 tacacaatga gaaaaaagtt cttccagttt ctacattact agaaggtcaa tataacgaaa
      961 gaaatgtatt tgcaggtgtt ccatgtgtta taggaaaaga tggtattgaa gaaattattg
     1021 aaatcaatat gactgaatat gagcaaaacg aatttaataa atcttgttct gttttaaggg
     1081 aatatattgc actttcaaaa acactttaga ttcaatattc tttcttacac taaaagcagg
     1141 taaatgactt attgaaaaga cttttaacta agttaatgtt taaatcattt gtacctgctt
     1201 ttaattttaa tactaaaata taaacctatt ttttaccatc aaattttata actttacttt
     1261 ttttatttac ctcttgagat aacaatcctt ttttcaatcc catcaataat tctaagtctt
     1321 ctggataaag atttattcca aattcctcta aacttgatac tatactattt acactcatta
     1381 ttttattttc tatcaataat tcaactgctc tttttaaata cgttgggctt gaacctttca
     1441 ttttgtctag tggttcttct atcaaccagc cttttttatc catttcattc ataagataat
     1501 tgtatttttt gtaatttatc ttttctagat tatatgctct aaaaatcata gcagatatag
     1561 gtacaatcca ttttttcttt agttctacat aataatctaa atcttctgga tgagtcaaat
     1621 cttttaaaaa actctctttt ggcaataaaa atgcacaggc atactcatct tctgaaaatt
     1681 gcttacttgg tattcttaat tcacttgata ttatatatcc aagctcacat gccaaagtat
     1741 aatttctaat tgatgctgac tttttgtcat tacccagaga tattagatat ttagattctt
     1801 tactaattct ctgtttttga gtaaagatag ttgccccttt tctatctaca ttcatacctg
     1861 atattataat ccctttagac tctaacaaac ttaccatatt tactattgga gtactattta
     1921 aatcccaaaa ctctctagtc ttatatgcaa gagattctat atcatttata ttaatatctg
     1981 acattacttt tctatttggc aagttttctt ttggaaactt aatataattc tccataaatg
     2041 aatatatttt atgtattata attatttttt ctctataaga aatctcttct actctaggta
     2101 gtttagattg agggttaaag tggctatcct ctacaacgat atttatattt tcatttttat
     2161 aaaaatactc ttttggaaaa tttaatatat tagataattt taatgtattt tctatatttg
     2221 gtacatactt attttcctca aaagctttta aatctttttt atttatttta gtttctttag
     2281 acaactgttc tatcgtcttt gctctaaaaa ttctagctga ttttaatctc tttccattaa
     2341 agtcttttaa attcatttgt tttctctctt tcaaaattaa attcttaatc tattggtata
     2401 aataagatta caattaataa tttatcatat tattgacaaa aaaacaaaag gtgaaaatat
     2461 ttttttatat tttcaccttt tagataagtt tataataaat attcctaatt actcaaaaag
     2521 ttttaaatat tctccatatc ctaaagcttc catattttct cttggtataa atttaagaga
     2581 agcagaattt atacaatatc ttaaacctcc aagttctttt ggaccatcat caaatacatg
     2641 acctaaatga gaatttcctt tagcacttct tacttcaact ctgtacatac cttgtgagaa
     2701 atcacttctc tcaatgatag atttatctat aactggcttt gaaaaacttg gccacccaca
     2761 tccagaatca aatttatctt ttgaagaaaa taacacttct ccacttacaa tatcaacata
     2821 aattccctct ttgttattgt cataatattc attttcaaaa ggtacttctg tcatatcatt
     2881 ttgagtgact tcaaattgta taggagttag agtctttttt aactcttccc tatttatatt
     2941 gtttccatcc caatggcttt ttacaaaatt atatcttcca gaccctttat aatacatatt
     3001 gtagtttaat ggtcttttct tataataatc ctgatgattt tcttctgctt cgtagaattt
     3061 agttgctgga attatatcag tcactatttt actatcaaat attttactat tttccagatg
     3121 ttctcttgac tctaaagcta tatttttttg ttcttcatca tgataaaata tagcagtctt
     3181 atattgagtt cccctatcac caaattgtcc accatcatct gtaggattaa tctgtttcca
     3241 gaagatattt agaagttcat cataacttat tatatcatca tcatatgtta tttgaattgc
     3301 ttctaaatga ccagtagttc ctgagcatac ctcttgatat gtagggtttt caacatgacc
     3361 acctgtatat ccagaaacta ctttaataac tccatcatac ttatcaaaag gttttaccat
     3421 acaccaaaaa catccacctg caaaagtagc aagttttatc attttcatca ctccttaaat
     3481 aaattatact ttatttattc cctaagaagt aacttaataa tctgcatttt attattttta
     3541 cctactcaat gaaatcatgt gtaaaaatac aattgcaaat tgaatctaat ttctataaaa
     3601 gctctttttc tttatccatc caaaagcatc ttttatatca caattttctc ctaaagacat
     3661 tagatattct aatgcatcac aatcatccat agctacatct ttttccatct taggatttaa
     3721 tctatagtaa ttatctccaa gaagtttttg agaaaataac tgactcattt gagaatttcc
     3781 ttctaaggta atagatataa ttggtaaatc gggttcttta ttaattatcc agtcaatagc
     3841 tccccattcg gtagtatctg cctttatact gttatataca tatccagtcc ctattgaaaa
     3901 aagtcttgta ttcttaagtc taaatccact gtcaatggca tgaattatac acgctaaact
     3961 cgggtcagtt gctattattc ctccatcaac atgacgatta tatgttggaa aaaataccgg
     4021 agcagcacta cttgccattg ctacatctac aactttgcta gtctttgtaa aagaatttgg
     4081 catattgtta taaaatactg gtttccatgc attttcctca tttcctatat aaaaacttgg
     4141 tatcattact aatttattta aatcttttaa ttctagttcc tctggaaata tacttaataa
     4201 aacttctttt aagtttttgt tttcatactt tggtctgagt atttctgcat aacttttatt
     4261 aaaaatatac tttgaatttt caattgagta aagttctttt atctctttag ggcttacacc
     4321 atacgcaagc cctagtgcaa ttagactccc tgtagatgta cctccaaaca tatttatatt
     4381 gtttaataga tttggatact cttttactat cctctcaagt atggatatac tcaatgcccc
     4441 ttttaatcca ccaccatcaa atgccaatat attaaaatta gaatttttca tatacatata
     4501 cccccacata aaacacctta tattttaaat tttaatcaaa ataatttcac tatagtata
//
LOCUS       DAEMIK010000223         4549 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEMIK010000223 DAEMIK010000000
VERSION     DAEMIK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.226"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(690..902)
                     /locus_tag="KPK87_003629"
     CDS             complement(690..902)
                     /locus_tag="KPK87_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539732.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(903..1319)
                     /locus_tag="KPK87_003630"
     CDS             complement(903..1319)
                     /locus_tag="KPK87_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539733.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(1389..1802)
                     /locus_tag="KPK87_003631"
     CDS             complement(1389..1802)
                     /locus_tag="KPK87_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539734.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            complement(2636..3265)
                     /locus_tag="KPK87_003632"
     CDS             complement(2636..3265)
                     /locus_tag="KPK87_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG2539735.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(3344..4306)
                     /locus_tag="KPK87_003633"
     CDS             complement(3344..4306)
                     /locus_tag="KPK87_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2539736.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
BASE COUNT         1584 a          712 c          442 g         1811 t
ORIGIN      
        1 tttattttgt aactacaata actgcctaag ttttgatttt cttttatctt ttacttctcc
       61 ttattaaaaa ataattcttt tactgttgta tctaaggctc taaacagttt aatcatcaaa
      121 tcattgtttg gattattaac cttacctaac ttactatata gctttttaac tgtatatctt
      181 taatagacct tttggtatgc taatccattt tataacacta attcttgaat caagcatata
      241 ttacccttac aaagcatatc ttataccctc tgtagagtta tgatagttga tgaacctgaa
      301 gaaattaccc ttacaagaca tatctgatac aacctcactt attagtttat atgtttcaga
      361 agtatataaa taacacatac ttacttccaa attcttaact gttttcttag aaaattctaa
      421 tatgatttcc ttacatagcg attataacag tgtcttatta gatttcataa aattatacat
      481 cctctttaca tttaaacttt aaatttttat ctattgttta ttaattaact gtatattcac
      541 tattttttat catttataat actcttctta ttatcttaaa acaattataa tctaacctta
      601 atcacatatt atataaatat ttttaataaa cttaataatt aaataactat atttcattat
      661 ccatcttata aatgtttata aactttccac tattcttctt caataaattc aaacaaattt
      721 tctactgtag tatcgaatac tttagcgata tccatagcta acttcaaaga aggattatat
      781 ttcccatttt ctaagtgtac aatagtctca cgtcttaccc caaccaattt agcaagttca
      841 ttttgcgtca taaggagttt ttctctgtat tctttaattt ttgttttcat caatattgcc
      901 acttagttac atatccccct tttatcaaag tatatataca acactaattt agataaagac
      961 tgtataaata aaactgcaat gaggattata cctgtatcta ggtttgatat atttatatta
     1021 cttgtataag gtgttgcaaa gaaaatagat attattacta tagaatatat cattattttt
     1081 attgcaatct tattaacctt tgatagtata aattcagatg attcatcaac taaatcatta
     1141 tattttttat tataaattga ataacatcca aaaactatta accaactcaa ccctggaaga
     1201 gaagcagtgc ttggaccttt aaaaatatat ttagtagcta atacagctat aaaaaaataa
     1261 gttattacaa gtaattccaa gatgagcttt gatcttacac cagtttctga tttagacata
     1321 taaaatcact ccttaaaagt tatatattct ctatattttt taatttttgt tttcataaat
     1381 attattactt agttacatat tcccttttta tcaaaatata tatacaaaat taattttgac
     1441 aaagattgta tgaataaaac tgctaagagc attataccta tatccaaatt tgatatgtcc
     1501 aaagctcttg tatgattaac tgaaaagaga acagctatta caaacataga gtataacatt
     1561 atttttgatg caatctgatc aacctttaac aacataagtt ttgatgcctc atcaactaaa
     1621 tcattatatt ttttattata catgagattg cacacccaaa aagctaataa aattataaaa
     1681 gtaaaccaaa taccttctcc tttaaaaata aacttttgta ataacaagtc tataaaaaaa
     1741 taggatagca tcattaactc tgaaacaaga tttgccttaa caccaagctc tgatttagac
     1801 atataaaatc accccttaaa agttatatat ttataacacc ttatgttata aatatataac
     1861 ttttccaaat aaattgcaat agaatattga aaatattcaa taatatcttc ataaatatat
     1921 tttataaaag attttataat aaacattaca atacagtact aaattagttt atttcatcta
     1981 tcatctcaat catatcattt actaactatt attctaatga gcatataaca ttgaaattta
     2041 taattataca acctaaatgc ctcccaatgt accctcaaaa acatcatttc ctgtaactga
     2101 agtataattg gtatatttta tcactgaaat aaaaaaacac tagtcaataa agactagtat
     2161 tttcaagtat tttatcattc tttttttaat tattggagac gaatcatcag aataatgccc
     2221 ataatttatc tatatgcaaa ttctaaattt aaaataaagt ttcagatgaa tttactattc
     2281 tatgctatga tacaacagaa aaaactgaaa tctatgagtg gtagcttcat ataatcctag
     2341 ttcctacagt aatcaaaact gaggtttatt ctatatgtaa ctaatgataa attttattat
     2401 aggagttatt agctctattt gtgttactat aagtagtaat tatatatatg ataatttttt
     2461 aacacttttc tgttggctat atagaaagtg gtttagaatt taaatttaaa taaaatttaa
     2521 attcaccaaa aagaaatcta tattagaaac cactactcta ttccaccaga ttgtaatttt
     2581 ttgttcttta tatcacaatg ctataccata ttaaattgaa aaataaattt taaatttatt
     2641 tgttacaatt cattaattca aaaaaatatt ttgagtcatg agttttgtaa agatttagcc
     2701 atgaatttaa tgcttctgat tcaaaaacat ctaaaagttt caatacttga aacgtaaatt
     2761 ccaacggagc gattcctgaa gccgtaacta aattttcatc actaactaca gattccgttt
     2821 tataatattt ttctccagag taatttggta ttaccatttt aagaaactct aagtcattac
     2881 ttgtatgatt tctaacatct aatagccctt ttttagctag gcctaatgta gctccacata
     2941 tggctgcaac tatagaatct tgtattaaag cttctttaac tttttctaat attatctcat
     3001 gattcatgga taaccaagta tttccccctg gcaaaattaa taaatcctta ctttccatat
     3061 taaactcatc aactgtaatt tcaggtaaaa cttttaaccc acccattgtt ataataggat
     3121 ttttatcaat tcctacagta attactttta gtggctttaa tcctttttta aaatatcttc
     3181 ctgtatttaa ttcagcagtt aaatatgcca cttcccaatc tgacatagta tcaaatacat
     3241 acatatatac ttttctattt ttcatctttt ttactcctta tgtaattaat ttatatatct
     3301 aattataaaa taacttaact gacatattca gtcagttaag ctattaaatt tgataataat
     3361 caattaattc tagtaaatct tcaattattt tcttcctaag gcttagtggt tcaattactt
     3421 gtattgtctt tctataaggt aaaagtatat ttggtatgta cttgcatatt acttcctctt
     3481 cgagcataaa tattatttta ttaggtgttc tttctttcaa gtagtgccct aaaaaccaat
     3541 gttggcttaa actatctaaa gaatctatac tcccttctac aactaattga attatatttt
     3601 gttcatcttt tatttcaggt ataattcctt ttataaaaaa ttcacttgct gaaaattctg
     3661 tcggtctgtt aaatgtattt gatgtttcta tgatttcact aatcctatcc actctgaaac
     3721 tacgtacctc atttctcata ttacaaaatg caactacgta ccaattatta ttccagtgaa
     3781 caattccata tggatttata attctatatt ttgattttgc tttacgacct gcatgatagt
     3841 tgatttctac agatagctcc tttaatatac tattctccaa ttttttaagt actgattcta
     3901 tagacacttt cccaattggt tttattactt caagggctct taagtggttt gttaaaatat
     3961 cttcttggtt ttgatttgaa tacattttta actttaatat cgatttatct aatgcttcgt
     4021 ctaagaagta tccttctttt ttagcaaata gagctgcatg aagtagagat ttttgctctt
     4081 ctatatcaaa aaaaagaggt gctttagtaa aattatttaa aaggctatac ccaccattat
     4141 gacctgtatc tgaaattata ggtactccac tcgcagaaag tacatcaata tatcggtaca
     4201 ctgttcttat atttatttct aatttttcag ctatctgttt tgctgtaatt tttttgtcag
     4261 agtttaacat ccacagtata gacatcatat tgtcaatttt agccatgtag tttcacctct
     4321 ttctatttgt tatataaagt tattatataa ctttttgatt gattttttac aaaaaaacat
     4381 tagtaaactg ctaataattt atactaagat ttatttcaaa tatttataga tagtcttttt
     4441 ctatagttaa gttatccatt aaattacata gtagtatatc cattttagtt gatatatttt
     4501 cagctacctc ccttgaatat tattacaata agtctaaatc ttctttctt
//
LOCUS       DAEMIK010000224         4502 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEMIK010000224 DAEMIK010000000
VERSION     DAEMIK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.227"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(21..734)
                     /locus_tag="KPK87_003634"
     CDS             complement(21..734)
                     /locus_tag="KPK87_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG2539737.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1026..2690
                     /locus_tag="KPK87_003635"
     CDS             1026..2690
                     /locus_tag="KPK87_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG2539738.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKHFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2797..3696)
                     /locus_tag="KPK87_003636"
     CDS             complement(2797..3696)
                     /locus_tag="KPK87_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG2539739.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3723..4361)
                     /locus_tag="KPK87_003637"
     CDS             complement(3723..4361)
                     /locus_tag="KPK87_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG2539740.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         1544 a          694 c          549 g         1715 t
ORIGIN      
        1 aatataagtt ttctatattt ttaaccaaca gatgttttct tcaaattaat cagatgattt
       61 agtatatctt gaacctttcc agagtccttt aaatatccta atgctccatt tatttgtgat
      121 tttaaatcta tttctacttc catatccaaa tcaacatttc ctttttttat agatatattt
      181 ttaagtgtta ttgggtaaag ataacttttt tccaaagtaa tcttatttcc attaacctca
      241 aatggaactt ttttattgta gttacttaat attttttcta gcattttatc acttagttta
      301 aattttccaa ttttcacatt ctcaagactt acaactaagt ttccatcctt taaagtaggt
      361 ctcaattcaa gttcatattg actatttacc aaaccaaata ccttatatgg tcctgctacc
      421 tttattttac catttttcat ttcaacaaaa ttattttcaa gttcattaat tccatgcttc
      481 tgcatcaatg tatatattaa atttttaaac tcttcctctg aaacagatat ttttccaacc
      541 attctaagtg gattttttac tacctccata gagtttatat atgagccttt tgataaatat
      601 tcttcatcta tgattttatt attttgtgaa ctaacatcat atctttcttt tggtgtcaag
      661 atatatacaa caattcctgc aagtactaaa acaataacaa ttaaacttaa caaaatcttt
      721 gcaacctttc ccataatcgt ctcctttaca aatctgtcat cagatttaaa ataaatttag
      781 ataactaact tcacataata ttcttaacaa tatacatact ataatacagt cttttaaaaa
      841 ttaattcaac caatctaatg ttttgtaaat aaactgtaaa aacatgtttt tagtataaaa
      901 gttaatattt tgcataatac tcttaattac acctttattt ttttctaatc ttggattaaa
      961 attaatatta tgcaatacag tgtagtgttc tgtattataa tcaaattaaa taggaggtat
     1021 agaatatgtc aaatgaaacg aactcatcta actttataaa gaatattatt ataaatgacc
     1081 ttgaaacagg aaaacatgac agtatcataa ctcgttttcc acctgagcca aacggatatt
     1141 tacatattgg tcatgcaaaa agtatatgtc ttaattttgg attagctaag gagtttaatg
     1201 gaaaagctaa tttaagattt gatgatacaa acccattaaa agaggatgtt gaatatgtag
     1261 agtccataaa agaagatgta aagtggttag gatttgattg gaacgagtta aattttgcat
     1321 ctaactactt tgatgaaatg tataagagag ctttaatact aataaaaaag ggtaaagctt
     1381 atgtatgtga tttgacacaa gaggaaatga gagaatatcg tggtactcta actgaaccag
     1441 gaaaagaaag tcctcataga aacagaacta ttgaagaaaa ccttgattta tttgaaagaa
     1501 tgaaaaatgg tgaatttaaa gatggtgaaa aaacattaag agccaaaata gatatgtctt
     1561 ctccaaatat aaaccttaga gacccaatta tatatagaat atctcattca acacaccata
     1621 atacaggaga taaatggtgc atatatccta tgtatgcttt tgctcatcca attgaagatg
     1681 caattgaagg aataactcac tctatatgta ctttagagtt tgaagaccaa agacctcttt
     1741 atgattggtt tgttaaagaa tgtgagatgg aaaatatacc tagacaaata gaatttgcta
     1801 gacttaatat aaataatact gtcatgagta aaaggaaatt aaaacaactt gtagatgagg
     1861 gtattgttga tggttgggat gaccctcgtg ttcctacaat atccggaatc agacgtaagg
     1921 gatatactgc agaagcattg cgcaatttct gtagtgaaat aggggtatca aaagttaact
     1981 ctacggtaga tagtcaaatg cttgattatt tcctaagaga aaacttacaa ccaaaggctc
     2041 ctcttactat gggagtttta agacctttaa aacttattat aactaattac ccagaagata
     2101 agattgagat gcttgaaata gagaataatg ctaaagatga atctcaagga aagagattag
     2161 ttcctttctc tagagaattg tatatagaac aagatgattt tatggaagaa cctgttaaga
     2221 aatattttag atttttccca ggaaatgaag tccgtttaaa aggtgcttat tttgtaaaat
     2281 gtactgatgt aattaaggat gaaaatggaa atgtagttga aatacatggt acttatgacc
     2341 ctgaaactaa gagtggctct ggctttacag gacgtaaggt aaaatctaca atacattggg
     2401 ttgatgctaa atcagctata ccatgtgaat ttagattatt tgaaccatta attcttgatg
     2461 atatacctga aaatgaaggt aaacatttct tagaacaaat aaatcctaat tcattggaaa
     2521 tattacaagg ttttgttgaa cctacacaga taaaagatgc taaacatttt gataaattcc
     2581 agtttgttag aaatggattc tttagtatag ataataaata tacaactgat gaaaaatttg
     2641 tatttaatag aatagttcct ctaaaaagtt cttttaaacc aggtaaataa tcttacaatc
     2701 aaaaaatgaa gacttaaaat ataaattact tctactataa ataaacttag ttagaagtaa
     2761 ttttttattt gagtcttcat catttacttt aaatgattaa cttttttcac tacagattcc
     2821 catgacttct attccatctt ttatgcattc tatgttaaga ggaaaatctg gtcctttttc
     2881 tccgtcaata tctgttataa gtgaatcttc acattctata tgcacctttt tagttttaaa
     2941 gtatagtatt tcatttccat tatattgatc taaataatca cctctaagca cacttatcaa
     3001 aactggtatt gattttggta ttggcattcc tttaaaaata attacatcca aatatccatc
     3061 atctatttca gccttatatg ctagatttat atttcctgct gttttgccat taaaaatcaa
     3121 cataagatac atatctccat cataatacat ttcctcagaa gatactttta tcttaaattt
     3181 cctcatatac aatgcttctt ctattccctt tatataataa gaaattcttc caaatgaatt
     3241 ttttaagtct gtgtttatcc tttgtgatac atccgtaaac ataccagcac ttgcaacatt
     3301 tataaagtac ttattattga ctttccctat gtctattttc tttggttttg aatttatgat
     3361 ttcatttata gcttctttta tatcaaatga aatttgaaga gcatttgaaa aatcatttgc
     3421 agttcctgta ggcaaaattc caattggaat ttttatgtct aattctttca ttgcatttac
     3481 tactaaatct acagtaccat caccaccagc tatcaatatg tgttcataag aactatcaac
     3541 ttccttaaat gcattcttta caggttcttt aatagttaat ctatagggta tcaataaata
     3601 atttttttct tgatataatt ctatgatagt gtctaaattt gataatatct ttttttcacc
     3661 tgaattagga ttgtatataa gttttacttt tttcattatt tctccttact cattttattc
     3721 ttttagtatc caaaatcttc atttaaaaaa atcacatgta ttctttctgg tgttaattgt
     3781 ctttttataa gtgtataact acagcatatt cttcctggac ttgaacagtc tatacatata
     3841 ccagtagtct tacatccagt atttgtactt aatcttttac aattggctgg agcagctatt
     3901 gtttttgttc tttttatagc ttcatctaca tctttaacta ttttatttac tcctgctata
     3961 actattactt tatcaggtcc atatatcata gcagcaaccc tattcccatt cccatctaaa
     4021 ttgtaaagtt ctccatcctc tgttatcgca ttagtacttg taaaataagc atctgcagaa
     4081 aatgactttc tatatatttc tttcatttct tctggtttga tatcttttgc atatctatca
     4141 agaaaattat atttttctct tataaaatca atcactcctg tttcaaataa ggtcatagaa
     4201 cctccacatc ctactagaga acctgcttta actatttctt caattttttt aagaagctct
     4261 tctttgttac ttactaaata ccctttcata ttattgtttt ccaaagattt tattgttttt
     4321 tctattcttt tttcattaag ccaatttaaa ttttgatcca ttttaacttc ccccttattt
     4381 ttaatatgta cttaattatt tataaacttt attttcttta taagtttatt ttagtacatc
     4441 taaaaaaaaa tacaatataa aacttaaata gttttacata attttttcca aaataactta
     4501 ct
//
LOCUS       DAEMIK010000225         4472 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEMIK010000225 DAEMIK010000000
VERSION     DAEMIK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.228"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..1310
                     /locus_tag="KPK87_003638"
     CDS             231..1310
                     /locus_tag="KPK87_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2539741.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKTTKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KPK87_003639"
     CDS             1634..2002
                     /locus_tag="KPK87_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2539742.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2916
                     /locus_tag="KPK87_003640"
     CDS             2119..2916
                     /locus_tag="KPK87_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2539743.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            3315..4205
                     /locus_tag="KPK87_003641"
     CDS             3315..4205
                     /locus_tag="KPK87_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2539744.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
BASE COUNT         1701 a          492 c          843 g         1436 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaatttat atggtttata gtttaacagt aggaaatatc
      121 gtatgagtaa gactatcata ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcact agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tactacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctgc taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ccatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaaacgacaa
     1081 aaacaaggct cgagtacgga attgattata tgaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttataatata taaaaattac atacccatta
     2941 ggtaaattat gtagtatatg tgtttacaga tggttataga aataaatatg taataaacta
     3001 cttaacactt aaaaagtgga tttgtccaga gtataataca aagcatgata tatataaatt
     3061 tttaagcgaa atacatcaaa agaaggatta gaattagtgg agacagaaca gctattatag
     3121 attataaata aatagtttta aaggagaggt actacgaata tagaggaagg taaatcatac
     3181 gttattcaag tattataaat gattagttta aaagtactat cgaataggag tctccatatt
     3241 taaaccttag taaaggtagt taaagtagta gtttaaaaac taaaaaatta tataaaggaa
     3301 ttaggtataa gaaaatgatt gagagtatac cagcaaaaca aattttacaa aaagtgaaat
     3361 ttgacaatac aagatggttt ggaattgact ataatatgaa tctctatcgt ggttgttctc
     3421 atggatgtat atattgtgat agccgtagca caatatacaa catagagaat tttgacaaag
     3481 ttagatataa agaaaatgtg atagaaattt taagtaaaga attgcgttca aaaagaaaaa
     3541 aaggggtagt aggcattgga gctatgtctg acacatataa tccttttgaa aagcaacttt
     3601 gtattacaaa gcaggcacta gatttgattt cggaaaatca ttttggagtg tctattgata
     3661 ctaaaagtag tttggtggtt agagatatac cgattttgca gaaaattaaa aaaaataatt
     3721 ctgctattgt aaagttgact ataaccactg caaatgatga attatcaaag aaaatagaac
     3781 cttatgtaaa tccaagttct gtacgatttg acgcagtgaa agaacttaat gattctggaa
     3841 ttttttgtgg catattatta actcctatgc ttccattttt gactgatact aaagatgaaa
     3901 tacgagcaat tgtagaaaaa gcacacaagg ctaatgcaaa atttatttat tgtatgtatg
     3961 gtgtaacaat gcgtagtggt cagcgagagt ttttttatga gcatttaaga gatatatcac
     4021 caaaattggt acttaaatat cagaaaacat atggtttaaa ctatgtatgt actgtacaaa
     4081 ataaagattg ttgtgagaag ttgttaagag aagaatgtgc acgttatggt attttgacgg
     4141 atatgaaaga gattataaaa gcgtacaaga ggtcggagtc atatactcaa ataaaatttt
     4201 tttaagatag gctttggatg agatataata gtggtgattg atgtgttgat attatagttg
     4261 attgttagag aggatattat agatgaatat tagtattgat attttaggta attgctagag
     4321 ctgatattat agatgaatat tagtattgat attctaggta attgctagag ctgatattat
     4381 agatgaatat tagtattgat attctaggta attgctagag ctgatattat agatgaatat
     4441 taatattgat attctaggta attgctagaa ct
//
LOCUS       DAEMIK010000226         4395 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEMIK010000226 DAEMIK010000000
VERSION     DAEMIK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.229"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            43..711
                     /locus_tag="KPK87_003642"
     CDS             43..711
                     /locus_tag="KPK87_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539745.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQVGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            870..1376
                     /locus_tag="KPK87_003643"
     CDS             870..1376
                     /locus_tag="KPK87_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG2539746.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1504..2520
                     /locus_tag="KPK87_003644"
     CDS             1504..2520
                     /locus_tag="KPK87_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG2539747.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2874..3578
                     /locus_tag="KPK87_003645"
     CDS             2874..3578
                     /locus_tag="KPK87_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG2539748.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGGIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3873..4352
                     /locus_tag="KPK87_003646"
     CDS             3873..4352
                     /locus_tag="KPK87_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG2539749.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1839 a          369 c          755 g         1432 t
ORIGIN      
        1 atattaggta ttttaaataa attttttaaa gggtgatttt ttatggaaaa tataactagt
       61 tattttacaa caatactatg tgtatttata tgcttatcat ctgtatttat atttactcaa
      121 ctagctagag tatttattaa taagaagaag attaatcaaa aaattaaatc aagaaacgga
      181 tttaggtatg atagagattt tattgaagcc agaagagaag aaatacatat aaaggataac
      241 aataataata aaaataagag taacaataag aaattaaagg aagaaaaagt atttaaatac
      301 gataatggtg atttatataa aggtgagttt gttgatggaa agaaaaatgg atttggaata
      361 tatatttttt caagtaagga aaagtatgaa ggattatgga aagatgacaa gatgcatgga
      421 ataggaaaat atacttatag agatggttca atatatacag gtgaatttaa atacggatta
      481 aaaaatggtt tgggaaaatt aacatatccc aataatgaca tttataaggg atatttttta
      541 gataataaga aaaatggtaa gggtgtatta tataaaaatg atggaaataa acaagtagga
      601 atatgggaag atgatgaaca atgtaaaagt ttagatttta aagatttaaa taataataaa
      661 ataaataatg tttatcaaaa tagattaaat aacaataaat taaaagttta attattctaa
      721 taaaaaaatc tttatttatg atggaatata ggatatatct atgttgtaat ttgtaaaatc
      781 tttctatatc atataaaatt aagtattgat tagtctatta cataaatata gaatataaat
      841 atatccattt aataataagg agaagagata tgaaagagta tgtagttgtg gatttagaaa
      901 ctacaggatt agatccatat aaaggttgtg aaataataga aataggaata acagaaataa
      961 aaaatgaaca aattgtaaaa aactattcaa ggttgataaa acctaagggg ataatttctt
     1021 catttataac agaattgaca catataagta atgaaatggt agaaaatgaa gaaccactag
     1081 aattagtttt acctagattt agaaaataca taggtgatag aactataatt gcacacaatg
     1141 cgaaatttga tttaaaattt ttaaattact atcttagaat gttagattta gaaccaataa
     1201 acaattatat atgtacagta gagttattaa aaaaatgtaa aagttataaa ggaaaaaaca
     1261 agaaacttga aacagcttgt gcttattaca acatagagaa tataaatgct catagagctg
     1321 acagtgatac attagcaact gcaaaactat ttttgaaaat aaaagatgaa tattaattat
     1381 aaatatagag aaaattagtg aaaatatcct ttataaaatt gacccttaag cttacaattt
     1441 tgttctttga tttattttgt agaacaatgt ataatgtacc ttatatgaag aagggagact
     1501 aaaatgggtt attacaatga taatgataat aataaaataa gacgtaaaaa aattagtaac
     1561 aacaaagata gtaataaaaa atctaaacat tcttatactt cggcaaataa aaaaaataaa
     1621 agtcctaata caagttccag taaaagaatg agtgaaaaag tagattcaca gaatcttata
     1681 aaaagagata gagatagttt tacaagagat aatgtaagac ctttaaatac agattacaat
     1741 caaagagaaa gaagaagagt cgagtataaa caaagagaga aaaaattaaa aagtaaaaaa
     1801 agaaaaaaac aaaaaaggat tagaatagtt agggcattat tatctattat gttagttgca
     1861 ctaattatat tttcagtggc aggtgcaata tttacagttt cagctataaa agggtcacca
     1921 gaagttacta aaaagttaat taggtctaaa tatataagta gtgaggttgt aagtataaaa
     1981 caaatgccaa atgatttaaa acatgcaata gtttccatag aagatgagag attttataag
     2041 cataaaggag tagatgttat ttctctagtt aggtcagtat taaacaatgt gtttacagat
     2101 acaacacaag gtgcaagtac tatagatatg caagtttcaa aaaacctgct tactagtaat
     2161 gataaaacta tgaagcggaa aataagggat atgtataatg ctaagcagat gaataagatt
     2221 atgactaagg atgaaattct tgaagcatat ttaaataata tgtatctagg taaaaatgca
     2281 tatggagctg caaagggagc tgaagtctac ttcggtaaaa aagttagtga attaaattta
     2341 gctgagtgtg ctatgttagc tggtatgaca aataatcctg caaggtatat tgaccatgga
     2401 gaggctaaga agagacaagt gattgtactg tataagatgc atcagcttgg ttatataact
     2461 gataaggagt atagaatagc aaaagctgat gacactccat ttaaatcaga aattgattaa
     2521 aattgtaaga atatagtatt ttcaatagtt taaacaaacg aaaatgtaat aaaaaaggtg
     2581 atttgccacc cgtttgccac cataattata atttaatagc aaattgtttt aatgtacaaa
     2641 tcagataata taatattgtt ttaaatacta cattatgcta aaatttaatt gtattttgta
     2701 aattaaaaaa cttataattt agttttctag ggttccgcat tttataatgg tcaggtccaa
     2761 gagaaaacgc acaattgttg tgtacacgga aggataaaag cctgggagat atttaataat
     2821 atttctatag gcttttttat ttttacatat tttaaatttg aggaggtttt ctaatgaaaa
     2881 aatttgttat tgaggatgca ttttggaatc tatttcctag tgcaaaaatt ggagttgtta
     2941 tttgtaatga tatagataac tctataaaag atgaagacta taagaaaata attttgaagg
     3001 gagaagaaga agcattaaaa tacttggagg actcagaatt tagcaacaac caagtgatta
     3061 aagtatggag agaagcattt aaaaaattta aaacgaaaaa aggagcaagg tcttctattg
     3121 aagcattatt aaaaagagtt cataatggaa ataatatagg aactattaac cctttagtgg
     3181 atatctacaa ttttatctca ttaaaatatg cattaccttg tggaggggag gatatagata
     3241 aatttatagg aggtattagg ctaactagag caattggtaa tgaggagttt atcccactag
     3301 ggacagatga aaattcattt ccatatgaag gagaaataat ttataaagat gatggtggag
     3361 caatttgtag atgttggaat tggcgtgaag cagtgagaac aatgcttact gaagatacta
     3421 acaatgcctt tttatgcatt gaattagtag atgaaagtag atttaaagaa tttgaaaatg
     3481 ctttaaatga attagctaaa atagttcaag aaaaattggg tgggaattgt aaaatttcaa
     3541 ttttagatat tgataataaa gaggtatcag tggattaata tggaggtgct gttgtaatgg
     3601 actattctag ttataaagat acttatattt agggtaagaa ggtctatttt agatataacg
     3661 attatcaaag gatataatat agaaaataaa aatgtagaat ataaaaagcc tttaaaataa
     3721 gaaaaaaatg tattttaaag gcttttatta tactcttttt tatttgcata attaacaata
     3781 aaaatatatt gttaattata atatttttat tgaaaaacga tataatataa tgtattatac
     3841 tatatataaa atattttaaa ggagttggca tgatgtctaa taaacttata aaaaatttac
     3901 taagtggtat attacaaatt ttattttttc ttttaggatt agtaattgtt gcaggaggat
     3961 ttaaaagttt tatgtacctc tgttttagtg gagaagcaac tctacaaggt actatttatg
     4021 gaatattaat gtttatttta ggtgtatcat attttatcat tataaaatca ttgatagaag
     4081 tattaggtag ttctgaatat agcctatttg taaaagagaa tgtaaaaaga tttaggatta
     4141 ttggatatct tttgctttta aactctctaa ttgagtttat ttcaactttt ggtactactg
     4201 gaaaaggaat gagatttcta gatttaggat ttggatttta ttttactgtt ccagtatttg
     4261 tatattttat aacttctctg atgagttttg taattgcaga tggatttgta aaggcaataa
     4321 agataaaaga ggataatgat ttaactattt aggtgtgaca ctttaatata taggagataa
     4381 ataacccgaa aagag
//
LOCUS       DAEMIK010000227         4234 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEMIK010000227 DAEMIK010000000
VERSION     DAEMIK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.230"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            535..1443
                     /gene="trxB"
                     /locus_tag="KPK87_003647"
     CDS             535..1443
                     /gene="trxB"
                     /locus_tag="KPK87_003647"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG2539750.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1533..3356)
                     /gene="typA"
                     /locus_tag="KPK87_003648"
     CDS             complement(1533..3356)
                     /gene="typA"
                     /locus_tag="KPK87_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG2539751.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3568..3810
                     /locus_tag="KPK87_003649"
     CDS             3568..3810
                     /locus_tag="KPK87_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539752.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3958..4149
                     /locus_tag="KPK87_003650"
     CDS             3958..4149
                     /locus_tag="KPK87_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG2539753.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1552 a          547 c          676 g         1459 t
ORIGIN      
        1 caaaacttta atttgaaaaa tgtaattata tggtatatca tataaatata agtaaatagt
       61 gctaatattt aaactattaa tcataattgg ttcaacaaaa gtatatagtt aataatgttt
      121 aatttgaaga aattgttagt gtgaaattac atatatattc aaattaaatt cataaatatt
      181 tcacataata atagtatact ataaacaaaa ggaggtatta aatatgggaa tagtacttat
      241 aacagcagca ttagttttgg ttatgtatgg actttcaatt ctatcatata tatcagaaag
      301 caatatatta gaagacactg taaatgataa ccaataaggt tttgaattat ataaataagt
      361 gagaactgta aataaaagtt aatttttatt tgcagttttt ttatatatat gtaaaataaa
      421 gttaattaat aaaaaataat gttttgttta tagtatatat ggataataat agtaaagtat
      481 aagaatgatg agaataatat ataagaaata ataatataaa gagaggtaaa cgttatggta
      541 gatatcattg taattggtgc tggaccagca ggtttaactt cagcaattta tgctatgaga
      601 gcagggttaa gcgttacagt atttgaaaaa aatatttatg gaggtcaagt tgcaagtacc
      661 tctgaggtag aaaattatcc agcagttcaa aagatttctg gggtagaatt ttctaataat
      721 atatacaatc aagcagttgc tcaaggtgtt gatattcagt ttgatgaggt tgaagagata
      781 aatttagagg gaaaagtaaa ggttgtaaaa acttcttcag gtgaacataa agcaaaggct
      841 gttattcttg caaatggagt tgaaagacgt aaattaggtt gtgcagggga acaagaattt
      901 acaggtagag gagtatctta ctgtgcaaca tgtgatggag ccttttttaa agataaagaa
      961 gttgctatag taggtggagg aaatactgca ctagaagatg ctttattttt agctaataat
     1021 tgtacaaaag tgtatttaat tcacagaaga gatagtttta gaggtgaaga agtgttggag
     1081 aaatctgtga aagcaagaga gaatattgag atactttaca gtcatggtgt agaaaaaata
     1141 gaaggagaaa agactgtatc gaaaatagaa gtaaaaaact taaagactga agaaaaaaga
     1201 actatagatg ttagtggtat ttttattgct ataggtctta aacctaataa taaaatgttt
     1261 gaaaatgttc ttgatttgga tgaaggtgga tatataattt cagatgagtc ttgtacaaca
     1321 tcagtagaag gtgtatatgt agcaggagat agtagaacta agtttttacg tcaaataata
     1381 acagcagcat cagatggagc tattgcagcc gttcaagcag ctaattatat aaatgttgaa
     1441 taatataaat ttcaaataaa aatagccata taaactgaaa taatttttca gtactatatg
     1501 gctattttgt taattagagg taaatgtacg ttttatttac cttgtctaga tttgttatat
     1561 cttaatctat catgttcatt taagaatctt tttctaagtc ttatagaatc aggtgtaatt
     1621 tcaactaatt catcatcttc tataaactct agtgcttctt caagagtaaa tattctagga
     1681 ggagataatt ttatagcatc atctgaacca gaagcacgta cgttactcat tttcttgttt
     1741 ttacaaggat tgactaccat atcatctttt ctggaattca taccaattat cataccttcg
     1801 taaacttcta caccagggtc tacaaacatt acagctctat cacttaaggc attaagagaa
     1861 taccccatag taacaccagg tccttgtgct attaaaacac catttcctct tgaaggtatt
     1921 tctcctttaa attcttcaaa tttttcaaat gaacgaacta aagtaccttc tccacgagta
     1981 gcgtttataa attcacttcg atatcctaaa agaccacgag taggtgctaa gaactctatt
     2041 tttacatagt ctccttctat tgacatagac tccatcatac cttttctcat gtttaattca
     2101 tttataatag taccagagta aacttcagga cagtttacaa caactctttc tattggttcc
     2161 attagttttc catcttcttt atgcattaaa acttcaggtt tagaaacacc aacttcataa
     2221 ccttcacgtc tcatattttc aagtagtata gataagtgaa gctctccacg tccagaaact
     2281 ttgtatccat cagtagtatc aagaggttca actttaagac caacatttac ttcaagttcc
     2341 ttttcaagtc tatctttcaa atgtctagtt gtaacgaatt ttccactttt tccaacgaat
     2401 ggagagtcgt ttactaagaa gttcatagaa agagtaggct cttctatttt tatcatttcc
     2461 ataggtagtg gactatctaa atcgcatata gtttcaccta tagatatatc aggaatacca
     2521 gctataacaa ctatttctcc actagtagct tcatcaactt caacttgttt taaaccttca
     2581 tatacagaaa gttttgatac ttttccttta gatacaacag aatcttctct acatatagca
     2641 acttgtgtat tgtttttaag agtacctttg taaattctac ctataccaag tctacctaca
     2701 taatcatcat atgcaagagc tgatatttgg aattgtagag gttcattatc gtaattagga
     2761 taagcttcaa cgtgatttac aatagtttta aatagaggag aaaggtcttc actatcatca
     2821 tccatttcaa gtttagcaat accttgttta gcaataccat atattatagg aaattcgcat
     2881 tgctcatctg ttgcatttaa gtctacaaat aaatcgaaaa cttcattaac tacttcttcc
     2941 gctctctggt cttttttatc aatcttgttt ataaataata ttggttttaa accgaactct
     3001 aaagattttt gtaaaacgaa tctagtttga ggcattgggc cttcactagc atcaactaat
     3061 agaataactg tgtcaacagt tttcataaca cgttcaactt cagaagaaaa gtcactgtgt
     3121 cctggagtat caactatgtt tattttgtaa tcttcatagt ttattgcaca gttttttgaa
     3181 tatatagtta tgcctctttc tttttcaagg tcgttactgt ccataacgca gtctttaact
     3241 acttcgttct ttctaaaaac accgctttga gaaaggaaag catcaaccaa tgttgatttt
     3301 ccagcgtcaa cgtgggctat aactgcgata tttataattt tatgtttttg tgacattttt
     3361 gttgctccct tcgattaaaa taacttatta attatagcac aatatattat taggtacaag
     3421 gaggaatata ttaaattgta ttaatatagt ttaaattgga taaaactgtt agttttgcat
     3481 ctgaaaatta ttctgtttaa aaaaattttt tagacaaata ataaccttaa tagataaaaa
     3541 gattaataaa agagtaaagg atgatatatg ttggaagtaa ccaaaataat aattaaatat
     3601 tttgcaagtg aaagtgatac ttttgtaaaa gaagaagttt gtaattttga agaacttctt
     3661 aagtggtctc ataaatttat tgaaggtaaa aagatagaag atgttttgta tataaagtct
     3721 aaagaaaact ataaagtaaa tgtgattaag tctttagaaa ttaaggaatt ggaaatagtg
     3781 agaaaacaac agataccacc gtacatgtag tattaataat atgtaaaatt aatatgtaat
     3841 actcacaata ggatataaat ggtatatatg gtaataattt atattaaaaa aacttttata
     3901 aagtttatat ttgggtataa aagtattaat tagacattaa ttaggaggaa cttaaatatg
     3961 ataagagtat gtccaaactg ttcaaatgtg gatgtaaata agctaaaaac tttggttgga
     4021 gaagaaaatg taaagactgg ttgtataggt cagtgtagag catttaaaaa agaagctgtt
     4081 ggaataatag acaatgagct tgaaataaag gaaacagaag cagagttttt tgaagcatgt
     4141 aaagaataaa ttttaaataa attataataa atagagcatc taaattgtat aagcattaaa
     4201 caatttagat gctctatttt tatatataca ttta
//
LOCUS       DAEMIK010000228         4150 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEMIK010000228 DAEMIK010000000
VERSION     DAEMIK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.232"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            421..1695
                     /locus_tag="KPK87_003651"
     CDS             421..1695
                     /locus_tag="KPK87_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2539754.1"
                     /translation="MQFIDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVMD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYADIPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYVKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1902..2219)
                     /gene="trxA"
                     /locus_tag="KPK87_003652"
     CDS             complement(1902..2219)
                     /gene="trxA"
                     /locus_tag="KPK87_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2539755.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2486..3238
                     /locus_tag="KPK87_003653"
     CDS             2486..3238
                     /locus_tag="KPK87_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG2539756.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3225..4106
                     /locus_tag="KPK87_003654"
     CDS             3225..4106
                     /locus_tag="KPK87_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG2539757.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT         1629 a          541 c          570 g         1410 t
ORIGIN      
        1 gtacatcttt tataacatat aaaatctttt tttctaatta tatacaatat attttccatt
       61 atgttctaat caatttcttt actattttta caactttatt agtcatattc ataattattt
      121 ctaattagtg tttaacattt tatgtaaaat ttataaaaaa atgtatataa attaaaataa
      181 gtgactatac taaaaatatg atacggataa acaataagtt caacctatct cccccaaaat
      241 caagtgagct acagtttccc ccaactgtag ctcattttcg tgtaatttaa taaacatctc
      301 aacaagaatt aattaacaaa accccttata aaaacataac atatattaaa caagctcttg
      361 attaaaactt gagctttttt aataaaaaac agaatagttt attagataag gggttttttt
      421 atgcaattca tagatgtatc taaatacatt gaaggtgcaa accttaaagt taaaactaaa
      481 aatggtgaaa ctgttaaaag aatatatttt aacaactctg ccactccatt agttttaaaa
      541 aacgtaatgg ataacttaaa ctgtgagatt ccttggctta cttatattaa tgctccagga
      601 ataatctcag aaaaaaatac tttaaaatat gaaaatgtac gctctactat cctcaaactt
      661 attgatggag acgaagaaaa ggattctgtt atttatgtca aaaatgctac agaaggaata
      721 aacttcttag caaaattttt taaagatgaa aatccagata aatttgttat taccacagca
      781 atggagcata tggctaatta cctgcctttt aaagttaatt ttcctacaaa agttgttgga
      841 attacagaaa aaggagaact tgatttatgt caattagaga atatacttaa aaattatgct
      901 ggtaatgtat ctctcgttac agttacagga gctagtaatg tcacaggtat aaccactcct
      961 atatatgaaa tagcaagaat agctcataag tatggtgcaa agatattggt ggatattgtg
     1021 caagtaattc agcataaacc tttttcaatg atgccacatg aatgtgatga gcatatagat
     1081 tttgttgttt ttagtgctca taaatgttat tctccactaa atggaggagc tctggtagga
     1141 cctaaaaaat tctttgaaaa atttaatcct gtactatatg gctcaggttc caccaaattt
     1201 gtaaatgata acaaaatact atatgcagac ataccccaac gttttgaagc aggataccca
     1261 gagtattttg gaactcttgc aatgggaaaa gcactgaaca ctttaaataa aattggtcta
     1321 aacagaattt caaaatacga aagagaatta tttttatatg ttaaagaaga aatgagtaaa
     1381 atacctaata taataatata tggagataaa tcaaataatg taattatccc aaatatatct
     1441 tttaatataa gtaatatgta ttacaaagat gtagcaaaat atttagttga taactttgga
     1501 atagaaactg gagctggagt agtaggtgca gatatttatg tacaaaaatt attaggtata
     1561 agtccaaaag aagcatatgt cagatttatg agggaaaatc ctgtaggtct tgttaggata
     1621 agcttaggaa tgtacaatac ttttgatgag gtggatagct ttatacatgc tattaaaact
     1681 ttggctttta aataagtttt ttctattctt ttttatcaat aaatagagag tagttttgtt
     1741 ttcacaaaaa agcaaattat atcaacagtc aatttcatta gcaattagac actcatttgt
     1801 aactatacaa actattttac actatacaaa caaaaaactc attacaatga gtttttttgt
     1861 ttatatatga gatttatttt aattacttca atttacttta attacatata tgattcaatt
     1921 ttgtttttta aattctcctt aggcataaaa cctattaatc tatctacagg ttttccatcc
     1981 ttaaatatta gcatagttgg tactgtagat acttcaaatt tctgagctaa ttctatactt
     2041 tggtctatgt ccaccttcaa gaagtttgct ttttcaacca tctcatttcc taaagcttca
     2101 tatattggtg atagcatctt acaaggtcca caccaagtcg caaagaaatc tacaactact
     2161 aaaccatctt tattttctac ttcatttata aattcattat tatttattat ttttaccata
     2221 atatacatct cctttattat cttcttgata ttaatatact acgccctata taacagttca
     2281 attaggcact tttttgtaac cgcctatttt taataggttt aatattatga taaatagatt
     2341 tataagatta aatatttatg agactaaata tttctaatat attttatttt tttcttaata
     2401 tagttgacat tttttccctt aaattataaa atcaaattaa taactacata ggtagttact
     2461 aatcttttat caaaggagtt tctatatgga taatataatt aatgaattac aaaatatagg
     2521 atttactaaa tacgaatctc aaatatacat atcattatta aaggaaagcc ctttaacagg
     2581 atatgaaata agtaaattat caggtgtacc acaatcaaaa gtctatgaaa atataacaaa
     2641 gctattaaac aataatataa ttatcaatgt tggaactgac cctattaagt acataccaat
     2701 aaaccctaat gaattattaa aaaataagaa aaaagaattt gacaattcta tggataatct
     2761 aaagaatagc ttacagatat taaataaaag taaaagtgta gaatacgtat taaacataaa
     2821 aggagataaa aatatattaa aaaaagcaat agaaatgata aaaaatgcga aacaagaaat
     2881 tataatttct tgcccttatg aagaaattct tgaattaaaa aaagaactcc taattgctta
     2941 caaaaaaaat attaatattg aaatactact tttagaaaac aaaagtctga aaggtcttga
     3001 taatattcat attcatggag gtaataaaac tacacttgaa aataaaatgc tttacaaagg
     3061 aattattttg attgtagata atgatgaaat attgacagga aacttatcag aaggcagtga
     3121 ggccatttca atttggacaa aaaactctaa cattttatat attggagttc aatacataaa
     3181 acatgaaatt tatatttcag aacaaataaa aggaggttat aaaaatgaac aaaattaaga
     3241 ttaaacaagt tgatgctttt acaactatac cttttggtgg aaatcctgct ggtgtcgtta
     3301 ctgatgcctc taacatttct gatgaaatta agcagaatgt ggctaaagaa atgaatttat
     3361 cagaaactgc atttgtttca gagtcaaata ttgcagattt taaagtacaa tttttcacac
     3421 caggtttaga agttgattta tgtggtcatg caacaatagg tactttccat gcacttttgg
     3481 aagaagggaa gcttaatact aataagagta tattttatca agaaacaaaa gctggtgtat
     3541 tatctgtaga gcttaagaaa ataaataatg aaaatatatt tatgatggaa cagaatacac
     3601 ctgaatttga aaatttagat aattgcagaa aagaacttgc acaaatgata ggtgtgcatg
     3661 aagatagact tcttgaatat cctattataa aggtaaatac tggactttgg tggcttgtat
     3721 ttggtctaaa atctttagat gatttaaaaa atatctctcc agatttaaat aaaatcaaaa
     3781 aatttagtgg tgataataat ataattggta taactccatt ttgtatagaa actatagata
     3841 aaaagtgtga ctaccattta agatctatag caccatatgt tggagttaca gaagaccctg
     3901 tatgtggaac tgggaacgct tgcgtatcat cttatatagt acatcacaat attttagata
     3961 aaactagttt tattggtgag caaggtaact ttataaacag acctggaaag gtatatgtaa
     4021 atataactag agaaaacaat gacataactt ctgtaaaaat tggaggaagt gcttatactg
     4081 ttttaagtgg tgaaattatg tattaatccc atacctaata aaaacaaata aaaatactag
     4141 ctaactaaag
//
LOCUS       DAEMIK010000229         4085 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEMIK010000229 DAEMIK010000000
VERSION     DAEMIK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.233"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            269..643
                     /locus_tag="KPK87_003655"
     CDS             269..643
                     /locus_tag="KPK87_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2539758.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            645..1502
                     /locus_tag="KPK87_003656"
     CDS             645..1502
                     /locus_tag="KPK87_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539759.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            1505..2143
                     /locus_tag="KPK87_003657"
     CDS             1505..2143
                     /locus_tag="KPK87_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2539760.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            3162..3896
                     /locus_tag="KPK87_003658"
     CDS             3162..3896
                     /locus_tag="KPK87_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2539761.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
BASE COUNT         1552 a          455 c          623 g         1455 t
ORIGIN      
        1 aacttaattt atcatcaaac aaatcattca tacaggatta ctttttcttt tagatattca
       61 attcttactt ttaataaata tttaatacat tctatcttat gtggttttaa aaagtgttta
      121 tggctatcaa caatctctaa tatttcattc atatttattt tgtcatagta atattttaca
      181 tcttttatat tgacaaaaaa gtgctctaag tgtatagtgt gtatatgata acatcacact
      241 gaaagtgata aaaagggggt gataaaaaat gaaagtgctc atttctaaca catcagataa
      301 gccattatac caacaaatta aagagcaaat taaagaagca atatttactg aagaactgaa
      361 agaaggggat gcaatgccat ccataagaaa ttttgctaat gatttaaaag ttagtatttt
      421 gactattaga cgagtgtatg aagaacttga aaatgaaggc tttcttacac gccaagttgg
      481 aataggaaca tttatttcaa ctggaaattt agaaatacta agagactcaa agagacgtat
      541 agttgaacaa aaaatggcag atatgattaa agatgcaaaa gcactaggaa ttacacagat
      601 agagttacaa gaaatgatgg atattctata tgaggaggat taatatggaa aatattttag
      661 aagtttataa cttaaataag acttataccg atttttcatt atctaatata agctttgcat
      721 taccagaaga ttgtataacc ggatttatag gtgttaatgg agcaggaaaa acaacaacaa
      781 tccgttcgat attggatgtc gtaaagcgtg atagtggaaa aataaaaatc tttggtaaag
      841 atttcgatgg aaatgagaag gaattaaaaa atcgaattgg agttgtcttt gatgattcat
      901 atttctatga ggagttgtca atggcagaaa tgaaaagtat tattgcacct tcatatgata
      961 attggtgtga agaagattat caacaaaata tgaagcgttt taatcttaac ccaaaacaaa
     1021 agattgatac actttcaaaa ggaatgaaaa tgaaatttgc tttagcatta gcattatcgc
     1081 ataaagctga gtttttaatt atggatgagc ctacaagtgg tttagaccca ctaatccgta
     1141 gtcagatact tgacatacta acaggatata tgaagaaatg tggtcgtggt gttttctttt
     1201 ccactcatat aacatctgat ttagacaaaa tagcagatat gattgttatg attgacaatg
     1261 gacgtattat attagagcgt gaaaaagata ctctattgga cgaacataga aaagtaaaag
     1321 gggatattca atttctaaat gatgaaacca aaaaattatt tttaaattta gaaattacag
     1381 gttatggttt tatagggatt acctcaaaaa ttaatgaagt gcaaaaaaat atgccagata
     1441 ttttaataga gcgtcctaca attgaagata ttatgcttgc atatattgaa aggagagagt
     1501 aattatgttg attcatttag ttaaaaaaga tattctgatt gtaaaaaaat atgtattgtt
     1561 gatattattt attgcaatag gaatcccatt atttatatta tggcgtttac ctccatttgc
     1621 acaaatattg ggatttgtac tatcaattat attttcagaa tttatgctgt gtcagtatct
     1681 attattaaag gagagtcaat attcaaaagc atctgtatta ctttgttcaa ctccttatcc
     1741 tagacgtggc ttagttcaat ctaagtatgt tttatttgca atggtattta tctattgtac
     1801 attagtttac tggattgaaa atttacttat tccacaaata ggaacttttg acttgacata
     1861 tattttgatt gttttattaa taagttctat tatttatgga gtttatatgc ctattcaata
     1921 caaattagga tatgaaacaa caaaattctt tttcgtgatg ataatcatgg cttcatcatt
     1981 tgtattacca ttaactatag ctcacagcaa ttttgaaatt ccatttatat atcagtatcc
     2041 atccatgata gtgaatattg tattgttcct tttaagttta attatactgt tagcttcaat
     2101 gtttatttct attaaaattt ttagtaataa ggagcttgta taaggtaagg gggactttta
     2161 tatgccacat ttaccagatt gggctgtttt actttgtatc ttacttccaa tagtatataa
     2221 agcttgtcag atatttaaaa gtaaaaaata gagtacatat ttatattgcc atttttattg
     2281 agtgaagtta gatggtatga atttaaaagg ttacttgaaa ataaagttac ttggtgcggg
     2341 agagtaacat cataatatta tagattatat gtttcttgtg aatcaatttg tattttttaa
     2401 tagggaatat ccagtaattt tattttccat tgattatttt tcaattatga gtatgattgt
     2461 gtgtattgta taccaccttt taagatgttt ttaaaaaatt atatcgtcaa aatataaatc
     2521 taaggaatcc tataaataaa gcttttcaat tttagaaaac taataaaaag taggaaatta
     2581 ataaaaagaa gctgtctcaa aatagagata agtttctttt tcattgctga aatgacgaaa
     2641 atataattga tttaatataa aaaagagtgc tttatgaact atttattttg aacagtagat
     2701 acacttgcac tcaatttagt tcctcctaag ctttatgaac taaaagttta ttttgaagct
     2761 tatttttatt aaaagagatt ttatttttct aaataatcat ctatatacag tattgcgttt
     2821 cttttattta ttcataattt attttcttat taaagttttt ctatattcat attttccata
     2881 ttactttact tttaatttta tacgaatata cgaattatca aaaataaaat agttattaga
     2941 aaaaaataaa agtaattgca aagcttgcaa ttacttactt ctatattaat tactatacca
     3001 taaaataaca aaaaaatcaa gtcgagtaat ccttcaactt aagtgatata ctaggtatta
     3061 ataattttta attaataaaa tataactaga ggaaaatcta cattttctca atttctttaa
     3121 ttaaattgac ctctaattaa gtaattataa ggagaaaaat tatgaattta gcaaagggat
     3181 tagatgtttt aaaaatttca tcaaatgtat tcggtgaaga taaagttatg tatattcctg
     3241 taatctacac agaagatgat gcaacactta ttgacacagg tcttccgggt caaggtgatt
     3301 taataattga tgctcttaat aaaagtaaca cgagctttga taggttaaaa aatataataa
     3361 ttactcatca tgatatagac catataggaa acataaatta tctaagagaa aaatctaaga
     3421 ataatatcaa agtatatgca tacaaaagtg aaatcagcta tataacagga gaagaaactc
     3481 cttttaaact ttatatgttg gaacaaatgg tagataaaat tgatgataag atgcttagta
     3541 tgctaaatgt tatgggactt ggatttaaat catcatatac taaagtagat gtgtcattag
     3601 ataaccatga aaaattgaat ctaggtgagg aaatagaagt tatacataca ggaggtcaca
     3661 ctagaggtca catatgtctt tatttaaaag agtctaaagt acttattgct ggagatttgt
     3721 tacaggtaga aaatggagaa cttaagcctg ttgatgtaat gcacagcaac aaacaagagt
     3781 taaaagatgc tattaaaaat ataagtaatt atgatataga aacgatagtt tttagtcatg
     3841 gtggattata tcaaagaaac ataattggaa cattgaaaaa cctaattatt gagtagtact
     3901 atatgattta caataatttc ttaaattact taaaattata tctaaagatg tagaatcaaa
     3961 attaaatata agtaataaga attttgcttt aaaattatac ttaagtgtta agggtgatac
     4021 gtagtattac aagtatttta gaatataatt ttttatagta attttaaagt tctacaatac
     4081 ttcag
//
LOCUS       DAEMIK010000230         4073 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEMIK010000230 DAEMIK010000000
VERSION     DAEMIK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.234"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..441
                     /locus_tag="KPK87_003659"
     CDS             76..441
                     /locus_tag="KPK87_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539762.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            519..1073
                     /locus_tag="KPK87_003660"
     CDS             519..1073
                     /locus_tag="KPK87_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2539763.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            1293..1967
                     /locus_tag="KPK87_003661"
     CDS             1293..1967
                     /locus_tag="KPK87_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2539764.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      2322..2418
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2472..2648
                     /locus_tag="KPK87_003662"
     CDS             2472..2648
                     /locus_tag="KPK87_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539765.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(2835..3074)
                     /locus_tag="KPK87_003663"
     CDS             complement(2835..3074)
                     /locus_tag="KPK87_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539766.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNIDRNWCKDKK"
     gene            complement(3219..4028)
                     /locus_tag="KPK87_003664"
     CDS             complement(3219..4028)
                     /locus_tag="KPK87_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2539767.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         1483 a          569 c          494 g         1527 t
ORIGIN      
        1 tacagttcat atatatttgt catatcacaa gacaagtgat ataatttatt atcattttac
       61 tgggggtgat atgctatgaa aataagagct gaatatacat gtcctcttga aattgtacat
      121 gacataatca aaggaaaatg gaaaactatc cttatatttg aattaagaca atctaataaa
      181 acattttctg aattgcaaca taatatttat ggaataaatc aaaaaatgtt gattcaacaa
      241 ttaaaagagt tacgtgattt tgggattatt gataaaataa gttctgctgg atatccatta
      301 catgttgaat attttcttac taatcgtggt aaaaaaatgt taaaagcagt ggaaataatg
      361 caagaaatag gaatcgaata tatgcttgaa catggtcaac aagaaattct agataataaa
      421 ggaatttgct acaataagta atcctataca taccaaaaag taagtaattt acatttttta
      481 caatagtatt ataataaaaa taaataggag gtataatcat gtctatagct ttaatattga
      541 ttgatattca aaatgattat tttaaaaatg gaaaatgtga attatttcaa tcagaagaaa
      601 ctgctgaaaa tgctaaaaaa atactactgt tttttagaaa aaacaattta ccagttattc
      661 atatacagca tatatcaaca gataaaacag cttctttttt tcttcctaat actcatggtt
      721 cagaaataaa taaaattgtt tttccactag ataatgaaga aattataatt aaacacacac
      781 cagatagttt ctttgaaaca gatttgcaaa gtatccttga gaaaaaaaat attactaatc
      841 tagtaatctg tggaatgatg agccatatgt gtattgatac aagtgtaaga acagcaaaga
      901 aacttcgata cgacataact ctaataagcg atgcatgtac aacgaagaat ttatcttgga
      961 ataatataga aattaatgca aatactgtac atcaagtatt catggcatca ctacagaaca
     1021 gttttgcaga tgtaaaaaac acagataaat ttttatctaa ctatcacaaa taaaaacata
     1081 cactatgtta aaagagcaag tttgatttat ttaagcttgc tctttaatca tttaaaatta
     1141 gatgctttct atcattttta tatacataat atacgtttat acattttata ttcatatata
     1201 actattatat aaattttaaa ctttactata ttcacataaa aaatgtagta ttatagtata
     1261 tataatatta tatagaaggg ggtgctagat aaatgaacca tcatatattg attcaatttg
     1321 aatatatagc aagaatcatt actgctggtt tctgtggggc tttaattgga tatgagagaa
     1381 agaatagact aaaagaagct ggaatacgca ctcattttat agtggcatta gcttcagcac
     1441 ttataatcat tgtttccaag tatggattta atgatatagt gggtactcca ggtattggtc
     1501 tagacccagc ccgcgttgca gcacaagttg tcagtggtat aggtttttta ggtgctggat
     1561 taatatttat tagaaatcaa tcagtaagtg gtcttactac agctgctggt atatgggcaa
     1621 cagccggtac aggtcttgcc attggctctg gattatactt agttggtatt gtttcaagta
     1681 tcattattgt tattgtacag ataacacttc atagagatag gaaatggatg aaactcccta
     1741 cagcagagca agtttctctt aaaatttcta acagcactga ttccatagaa tatattgaag
     1801 gtaaatttaa aaaatctaat attgaaatta taaacatgaa tattaaacgc cttgatgggg
     1861 attggttaga agtagttata tatactaagt tacctaaagg atataaaaaa acaaatttat
     1921 taaaattatt tagcgataat cctcttatag aaactttaga aatataatat aaaaattgaa
     1981 gcttaaaaat aataatatat ttaatcaagg tcaatatata aataattacg ggatgagtct
     2041 atataaaatt catcccttgt tttttgccat tcatatatat tttttcaaga tatttttgtt
     2101 catctattat ctagccaagt agctcttgtg aactaaactt tttaatacaa aaacttcaat
     2161 acaaaactaa atataacttt tattaaacat tctacatata tagtttgttt cttgagtgaa
     2221 aaatagattt catattgtta agatactgtt aaatatttca attttctgac aagtatgttg
     2281 aacaataact tatcctagtg tattatttta gttgtagaat aaaactaaat cggcaaaact
     2341 agagaaattt agtgacgcaa agctataggg actaacgctt ataaaataaa ctatgagtta
     2401 tgtcagccag ttgccaaaaa gatattgttc tttttgtttt atgaaagttt tttaggggga
     2461 tagtaatata tatgtttaga gatgaaatgg ataaatgtac acacatgtta actgcttata
     2521 ttagtagttt atatgattat tgtgatttta tagatacaca gctagatgat tttatactag
     2581 agtacggaga aaatgtagta gaatcttgtt tacatcaagt gatggtattg gtaagtaagt
     2641 ataattaaat agaaattgtt ttctagagaa ccctttttag ttttatctac atttaaaaat
     2701 gttattatat taaaaaacta atctccccta tatgcaaagt ttcttccata taacaatttc
     2761 ttcccattta tactttttca cttatatctt taataaaatt caacttcaaa tattaatata
     2821 cttcaaaaat tattctattt cttatcttta caccaattcc tatcaatatt atcctttata
     2881 taggttctaa ctatttcgcc acaatctaaa cataaatctg aatatatagt agattgtact
     2941 ggaaaagttt tatttataac tttatctaat tttccatctc ctctatattt taatccaata
     3001 gtttcagcat atatgccacc agttgttaaa attccctttt caatatttac acttccacaa
     3061 ttagaacatt tcacatttat aaccccctaa acaattattt tttcatgcta tcttataatt
     3121 taaaatataa tttgctaccg attttataac acagtggcaa atcggtggca aatcaacatt
     3181 ttttcattct atttaccatc taatccattg aaatatggct atttagttat aggaacttga
     3241 atttctgtta caaactcctc tacaattcca gtctcatgta tatctatctt gtagatttct
     3301 ataggatcag caattatctt ataattgttt tcttctataa atttaaacac cttctccata
     3361 tacatcttat tattagaatt actcccagta taatttaaag ttacataata tccttcatca
     3421 aaaactatat tatatacttt ctcattactt tccagaaaac aaaatacaga tttaaactca
     3481 ttaaatatac ctttattaat tgcttcagta gaaaaggcag aaccaatatt gttgtttcca
     3541 agtatattaa atctatcttc atattctttc tgaagttttt gaattaaaaa atcgaagtct
     3601 tcatccctct ttatatcagc atttaattct aaagcttttc ttttttctat atatacaacc
     3661 tctatctcat ctatttttgt attttgaatt acactttcaa ttgaactcaa tctttttata
     3721 atattttcct tatggcttat aaattcagct atttttttat caattaaatc gagttcttca
     3781 tttaaaatat ttttcgtaga ctctatactt ctgtcatcta ggtattcttt tattttcttc
     3841 attggaaagt tcaagcttct aaattctttt attaaattta atttccatat atctgatata
     3901 ttatacattc tatatccatt tatatctcta actggtctta gaatgcctat ttcttcataa
     3961 tacattaatg aatctctacc tattccatat attttagata tctctcctat tttatagtaa
     4021 tctttcattt atataataaa ctcctccgaa atcttatttt tattttaaat caa
//
LOCUS       DAEMIK010000231         4029 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEMIK010000231 DAEMIK010000000
VERSION     DAEMIK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.235"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            116..1714
                     /locus_tag="KPK87_003665"
     CDS             116..1714
                     /locus_tag="KPK87_003665"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG2539768.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1949..3976)
                     /gene="bclA1"
                     /locus_tag="KPK87_003666"
     CDS             complement(1949..3976)
                     /gene="bclA1"
                     /locus_tag="KPK87_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG2539769.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTGATG
                     PTGATGEIGPTGATGPTGNTGVTGEIGPTGATGPTGNTGVTGEIGPTGATGPTGNTGV
                     TGEIGPTGPTGNTGVTGEIGPTGVTGPTGNTGATGSIGPTGVTGPTGVTGSIGPTGAT
                     GPTGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSIT
                     VDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGG
                     VLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT         1175 a          739 c          626 g         1489 t
ORIGIN      
        1 tttatataat attttttcac taaatttttt tctttttaca acaaatactc actacctttt
       61 gatataatat ttttatcaat tattattata ttcaagtttt gaaaggagta ttaaaatgag
      121 tttattagtt ttcggacata aaaatccaga tacagattca atatgttctg caatttcatt
      181 agcacattta aagaacaaac taggaataaa agcaacagct tatgctttag gagatatcag
      241 aaaagaagct aaatatgcct tggattactt taaggtagat gcacctgaaa ttttagataa
      301 tgttagaata caagttagag atttaaatta tgataaagta gtacctttaa caccaacttc
      361 atctatatta gaggcttata accttatgga tgaaaaaaat gttaaaacac tacctgtaac
      421 ttatgatgat ggaacttttg ctggtatcat tactatgaaa gaaatagcta aaaatctact
      481 tcatcaacac tttacaacta tacacacatc actatcaaac atttgtaaaa accttaatgg
      541 tactattctt gtggactgtg gtgaagatat aaaaggtaga gtagtcactt tatcatttgg
      601 aatggagact ctaatagaga ctttaaaaga aggagacata gctattgttg gagatagata
      661 tgatagtatt gaatatgcta ttgatacaaa agttaaactt ttaatactta ctaatcatac
      721 agagatttca aaagatttgc tagctcttgc taagataaat ggagtgtctg ttgtttctgt
      781 tgaaagtgac aattataaaa cttctaatgt aataaatcaa tgtagtttct tagactctat
      841 agaagctaca gaaaatttaa ttacattcaa agaagatgat tatatggatg aaataaaaga
      901 gattatgcta gaaacaaact tcagatctta tccaatatta gatgatgata ataagttttt
      961 aggtttagtt tcaaaaggtc atctattaaa cccatctaaa aagaatgttg ttttagttga
     1021 ccataacgaa tatgctcaaa gtgctgatgg aattgaacaa gcaaacatag ttgaaataat
     1081 tgaccatcat aaacttggtg gtatttctac tgatgttcct atgtcattta gagttatgcc
     1141 tgttggatgc aatagtacta taatttatca aatgtacaaa gaaaataatg ttgagatacc
     1201 ttatgaaata gccgggcttc ttttatctgc tatattatca gatacattat tatttaagtc
     1261 tccaacaaca actgatatag ataagaaagc ttgtgaagaa ttaagtaaaa tagcaaaagt
     1321 agacatggaa aaatacgcta tggatatgtt taaatgcggt acttctttag atgaatatac
     1381 tatagaagaa atagtaaata tggactttaa agaatttaat atgagtggac atagagttgg
     1441 tattggacaa gtatttacct tagacataga ctctatattt gctaaaaaag atgagttttt
     1501 atcttatata aattcgactg attatgataa attaattctt tcagttacag atataatcaa
     1561 agaaggttct tatttaatct ataaagcaga agatagctta attgctaacg catttggtgt
     1621 tgaaggtgtt caaggcacat ttgcaccagg tgttgtatca agaaaaaaac aattagtccc
     1681 taatttaact actgcaatca aaaattttaa ataatataaa atttttaaat aacgaaaact
     1741 gtctcaaatc tatatttgag acagtttttt attatatata tattgataat aaagttaaat
     1801 aaaaatccat aaatttaaaa tttttatgaa tatattttat tattattgtt attttattca
     1861 ttctaaaata ggtaaaactc aatatataaa aatacttttg tcatttaaat aaagcctaaa
     1921 tagtttttaa gattatttta gacacgtttt aagtcataac agtatcagct attctaaata
     1981 tcgtcaaagt agccgcagtt cctgctgatt gcccatttcg tatccctact gttgtagatg
     2041 aaaaattatt tattgtaaaa gtagtcccac caaataaaaa accagcaaaa tatccagaaa
     2101 gtactcctcc gcctcctggc tcagtagtaa gccctgccaa atcttttcct cctaaaattg
     2161 ggaataggat agcgaaagaa tattgattta tacttgatga acctggttgt aatgctactg
     2221 aaactgatat ataatacaca ccattttctt gtactgtaaa cgtaccattt gcatctactg
     2281 ttatacttga actatttcct attattgttt gccaacctgt tatagcatct ccattattta
     2341 gtgtttgtgg agatggtgca tttacgagga agttagcaac tggctgagag gaattacctg
     2401 ttgctcctgt tggacctgtt gggcctgtta ctcctgtcgc tcctgttgga cctgttgctc
     2461 ccgttggtcc tatacttcct gtcactcctg ttggacctgt tactcccgtt ggccctatac
     2521 ttcctgtcgc tcctgtattt cctgttggtc ctgttactcc tgttggccct atttctcctg
     2581 tcactcctgt atttcctgtc ggacccgttg gtcctatttc tcctgtcact cctgtatttc
     2641 ctgtcggtcc tgttgctccc gttggtccta tttctcctgt cactcctgta tttcctgttg
     2701 ggcctgttgc tcccgttggt cctatttctc ctgttactcc tgtatttcct gttgggcctg
     2761 ttgctcccgt tggtcctatt tctcctgttg ctcctgttgg gcctgttgct cccgttggtc
     2821 ctatacttcc tgtcactcct gttgggcctg ttgctcccgt tggtcctatt tctcctgttg
     2881 gccctatttc tcctgtcact cctgtcgctc ctgttggacc tgttgctcct gttggtccta
     2941 tacttcctgt cgctcctgtt gctcccgttg gtcctatttc tcctgttggt cctgttgctc
     3001 ctgttggtcc tatacttcct gtcactcctg ttggacctgt tgctcccgtt ggtcctattt
     3061 ctcctgtatt tcctgttggg cctgttactc ctgttggtcc tatacttcct gtcactcccg
     3121 tatttcctgt cgggcctgtt gctcccgttg gtcctatact tcctgtcact cccgtatttc
     3181 ctgtcggacc tgttactcct gttggtccta tacttcctgt cgctcctgta cttccggttg
     3241 ggcctgttac tcccgttggc cctatacttc ctgttgctcc tgtatttccg gttgggcctg
     3301 ttggtcctat gcttcctgtc gctcctgtac ttccggttgg gcctgttact cccgttggtc
     3361 ctgtacttcc tgtcgctcct cttggtccca ttggacctgt aattctaatt ggaagacact
     3421 cacatttagg ataacaatta tctataattc tatcttcaaa tgttacaagt atatacggtt
     3481 tgttacaacc tctactagat gtaaattcaa gaatctgata agggtaattt aaagataaag
     3541 ctaacccata attaggatat ttattcattg cccatgcttt tatagttcct gtaatattta
     3601 aacaaatata gttggatgta tcacttatcc caactggtaa atatccccta atattttcca
     3661 tagacactct atcattccat gttatagttc gtaaattata atattcactt attcttttaa
     3721 tttcaatatc tactttttct ttaaaaatat gattcttatt tgaatcaata tgaataaata
     3781 actctgcttt taatatactt ttgtctttta atatatctgg tattctcact ttaaaaaaag
     3841 taatcaactt ttcttttaca aaactatttg aacatttact tcctattaag caataatcta
     3901 aattactaaa gtttttatct ggatattttt tagatataaa agtatcatca tttaaataaa
     3961 gtataatatt tctcatttat aatccctcca ttttgggtct atatcatact atgtttaaaa
     4021 tacatgctt
//
LOCUS       DAEMIK010000232         4003 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEMIK010000232 DAEMIK010000000
VERSION     DAEMIK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.236"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            737..3789
                     /locus_tag="KPK87_003667"
                     /pseudo
     CDS             737..3789
                     /locus_tag="KPK87_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
BASE COUNT         1542 a          561 c          636 g         1264 t
ORIGIN      
        1 cattatactt aatataaatc gatataaaaa caattacaat attatattga ttattcgaaa
       61 ttatatataa aattcagaaa aaaattggtt tacataataa aaataaagtc aactaaaatt
      121 attgatttta gattaaagta ccggattaag catttaagta gattttaagc ttgatttgat
      181 tagaaaggta caaactatca ccgaaaaaaa ttaaacccat ttaaaaagca atataaaagc
      241 ttgttatttt tgagaaaaat agaccctcta aactcaatta gataaaaata aaatacagaa
      301 aattatatct ctgaatatct caagtaatta attttaaatt ttacgatatt ttgtttgatg
      361 atttataata taaaagcata ctcttaatct gctcaaatta aaaagaataa aaaataacaa
      421 aaaaatcact gacatttcac tgacaaattg taattttgtt attataaata cattgaaaat
      481 acttgattat tgaaaaatgg cataaaataa tacattttat gccatttact attatttgat
      541 agtttttaac ttatgacata agatattttt taacatatac aataaaatat aaaaaaattg
      601 atattgcaaa taaaaaaaat acatattaga tgttatagaa aaaaagcgca cttataggct
      661 taaaatgtca cataaaagag ttttaaagaa taatatatta tgaacacaaa ttttgtaatc
      721 acaaaggagt gataatatgg caacagtatc aggacaaata gtttttgata gaaatagaag
      781 tgctaccatt gattctggag actcaggaat agccaatata ccagttgtat tacaagatat
      841 agcaactgga gttagacttg tggttctcac agatgcatca ggaaattatg tttttactaa
      901 tgttccaaat ggtaactata gaattgtaga agcttttgga actacaggag gagtaccaac
      961 accaggaaat tttgcaacag cgatagtagg tccagttcca attgcagaaa tgccacctat
     1021 tagttctgtg acaaatcctc ctattggttc aacaaactta gatggtacaa cacctaacat
     1081 actattaata acagtagcag gttcagatat aacaaatcaa aatatattaa atgggcctgt
     1141 gatttataca cctatagaga atatattgga ttcttgcgta tctgtatcta atataaacct
     1201 tattacagat gcagataacg gaacattcgg tttttttcca ccaggtacac caagtaatac
     1261 aggacctgaa atagaacctt acccaaatgt gacgccagat tttacgtatg ttgtaccaga
     1321 cccaactaag tttacacctt tagatggaga atatacagta caaaatatta tgacaaatgc
     1381 aatgagtaat gttataggag cttggtggcg tattgctgac catactacag gaaatgaaac
     1441 aggaagaatg atggtggtaa atggttttaa tcctggagct gtatttttta aatcaacagt
     1501 acaagttaca cctaacacta attatttatt tagcacttgg attttaaacc tttttaaggt
     1561 aacaggatat ccaccaccac agctaggtgt aagaatattt gaccaaaata ataatatact
     1621 atatcaagca acattaggag ctcaaattcc agttaataca aacgcaccag aatggaaaga
     1681 agtaggtact gtgattaatt ctcaaaataa taatcaactt acagtagaat tttttagtga
     1741 aggagaaagc gccattggaa atgactatgc tatagatgat gtttctttaa gagaaatagc
     1801 tgttccaact tttactccaa taaaaacaag taacacagaa actgctaatg taggtgatat
     1861 aatcacatat acagttacat tagataatac ttgtgatagc cctttaacta atgtattttt
     1921 tagggattta attccaaatg gtttagagtt tatacctgga actgtaacgg ttgatggagt
     1981 atctgtacca ggagtagacc ctaatacagg tttcccactt ccagatattg gaggaggaac
     2041 aggggttgaa gtaacttttg atgttgtggt tgaaagtatt cctaacccaa atcctactaa
     2101 taatatagct aatatagatt actcttatac acctgtagaa ggcggtatac caaatgattt
     2161 ttcagtagat tctaatccag ttcctgttga agtaatctca gcagatattg aagttacaaa
     2221 attatctgaa cctactatag tgaatccagg agaggaatta atatacacta taaaagtagt
     2281 aaataatgga ccattcccat ctgaaaatgt ggttttaaca gatgatgtac cagcttctat
     2341 tataaatcca gagtattcat tagatggagg agtaactttt caaccatgga ctggaagttt
     2401 aaatataggg acattagaag taggagagac aagagttatt ataattaggg gtattgtaaa
     2461 tccatcaaca gtaggaatta taacaaatac agctgtagta tcttcaacta cagcagaccc
     2521 aaaccttaat aataatacat ctacaataga gacagaagtg aatttattag cagatatttt
     2581 agtaatgaaa acagcagaac ctaattcagc tgttccaggt actttattga gatatactat
     2641 tcaagtagaa aacttaggac cagcaaatgc tgaaaatgta atattaaatg atgatattcc
     2701 agcttctatt ataaatccag aatattcatt ggatggagga gcaagttttc aaccatggaa
     2761 tggaagtctt aatataggaa ctttaaactc tggaatttca ttaacagttt taattcaagg
     2821 aacagttagt ttaaattctt cagaatatat agtaaataca gcaacagttt cttcaactac
     2881 acctgaccca gatttaagca acaatatatc taccattatt acaccagtta acccacaagc
     2941 aggtatttct attataaaag tagctgacga ggatgtagct gttccaggag aagaatttgt
     3001 atatacaatt gaaattttta atgaaggacc atctaatgct acgaatgtag ttttaacaga
     3061 tgatatacca gatgttattt taaatccaga atattcattg gatggaggag caactttcca
     3121 accatggact ggaagtctta atataggtac agttgcacca gggcaattaa taagaattat
     3181 cataaaggga ttagtaagtt caactgctac aggagatatt actaatacag cagaagtaag
     3241 tgcagatgta ccagaaccag ttacggattc atcaacagtg acaactccta tagttccatc
     3301 agcagatata gaagtaataa aaactagcaa tatggataca gcagttccag gagaaacttt
     3361 tagttatact atattagtta tactataaca gttacaaatt taggaccatc tgctgcacaa
     3421 agtatcgttt tgacagatga tataccagat gttattttaa atccagagta ttcattggat
     3481 ggaggagtaa ctttccaacc atggactgga agtcttaata taggtacact accagctgga
     3541 actttaagag tgattatatt aagaggaatt ataaaatcaa catgtcttga tagattaaca
     3601 aatattgcag aagttacttc aacaacacca gattcaaacc ttaacaataa tatatcaaga
     3661 gtgcaagtag aaataaaaca atgttgttgt aatgattgct gttgtaacaa ctgtagtaat
     3721 tgttgttgca atgattgtgg ttgtaaagat tgttgctgta attgttgtaa aaatgacttt
     3781 tggtgttgat tttataaaat aattttgtat accttaaatt aaactgaaat aaaaaataaa
     3841 ttcacatatg agtaaaatat tttataaata ttaataaatt ttctatcttt gatatggacc
     3901 acattcgtaa tactagttat taaatgaagt ggtccttttt aattcattaa aattatttat
     3961 tctaattaaa ttttaaatgt attaaataat tttaattttt tat
//
LOCUS       DAEMIK010000233         4000 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEMIK010000233 DAEMIK010000000
VERSION     DAEMIK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.237"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..869)
                     /locus_tag="KPK87_003668"
     CDS             complement(<1..869)
                     /locus_tag="KPK87_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG2539770.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRA"
     gene            complement(1145..3799)
                     /gene="adhE"
                     /locus_tag="KPK87_003669"
                     /gene_synonym="adhC"
     CDS             complement(1145..3799)
                     /gene="adhE"
                     /locus_tag="KPK87_003669"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG2539771.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYANGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1253 a          723 c          460 g         1564 t
ORIGIN      
        1 gctctgttga ttatttgttt tgaattacta aatacaacag tttctggaat tgaaaaaatt
       61 aaattccagt cttttatata attattttca ctattcccat tatccatagt ggcatatcct
      121 aaatatcttc tttcttccaa catatttaat acagttgcac ctgttttatt ttcttgcaat
      181 tcttttttta atctatttac ttcttgaata tcattatcct gttttaacaa tttataaata
      241 ttttttgata ggctatcccc ttgtggatga aggataattg taccattatt ttttacaaca
      301 taagatatac tattattttc ataaaaagat atattaataa gtttcatcag agtattgata
      361 ttgtaagtac aatataaaac tccaactact ttatcattat ttaataatgg tactgaaata
      421 gcaatcttct ttatagactt atttacttca tcaacatatg gacttgaaac atacttctta
      481 cctttaattg agtttttgaa aaattctcta tctttcactg aaaacttatc acctttggta
      541 ttttcagcat taccatttat atcaactatt gccatagtgt aaaatttttg attttccatt
      601 tcatctaaaa gatttttcat tttttcctca ttaggtatat ctggatttga agcataatag
      661 ttagaaaaaa tatttaaaac atctaaatct ttttcaattt gtctttgtgc tagttgagag
      721 ccttgtactg caacttcttt tatatgtatt tttgcctctg acataagaag atgttttgta
      781 tcttctatat agatataccc cattgtcaaa aataatgatg ttaaaaaaag aattgtaatt
      841 aatgtaaaac ttttatttct ccctgtcatt cgactcctcc cctaattagt caatacttca
      901 tatagataat atatcctttt tataccaatt catcatttat attgtatata actattctta
      961 aatgttacac ataaaatcaa cataataacc ataaaaacat tcttttatta ttataataac
     1021 actatttaat tgaattttaa attaaagaat aaaaactatc cattttctca aacaaaaaaa
     1081 ctcttataca ataaaacttt gttttatgta taagagtttt tattttatct attttatagc
     1141 aaacttatct tccataatat gaatttaaat acatatcttt tatttcattc ataagtggat
     1201 atcttggatt tgctcctgta cattggtcat caaatgcttg aattaccatt tcatctattg
     1261 aatccataaa cttagattct tcaacgccaa actcttttat agatttagga ataccaactt
     1321 taagttttaa atcttcaata gctaccaata aactttggaa tttctcttca tcagaatttc
     1381 cttttaatcc taaaaatgaa gctatttcag catatctctt taatgcttct ggatatttat
     1441 attgagagaa tgctcccatt ttctttggtg catctgaaga attaaatttc ataacttctg
     1501 ttatcaaaag agcatttgca acaccatgtg gtacatggtg gaaagcacct aatttatgag
     1561 ccatagagtg acaaacaccc aaaaatgcat ttgcaaaagc catacctgcc attgtagaag
     1621 catttgccat tttttctcta gcttctgggt cttttgcacc attatcatat gcacgtggaa
     1681 gatattcaaa tatactctta atagcttgta aagctaatcc atttgcataa tcggttgcaa
     1741 gcatagatac atatgcttct aatgcatgag ttaaagcatc tataccagaa gcagatgtta
     1801 agctttttgg catattcatc atcatatcag aatcaactat agccatattt ggcatcagtt
     1861 cataatctgc taatggatat tttactcctg tatcttggtc agttataaca gcaaatggtg
     1921 ttacttctga accagtacct gctgatgttg gtatagctac aaaattagct ttttcaccca
     1981 ttttagggaa tgtatatacc ctttttctta tatccataaa tctcatagct aggtcttgga
     2041 aatctacatc tggatgctca tacaatgtcc acattatctt tcctgcatcc attgcacttc
     2101 ctccacctat agatattatt acatctggat taaattctct catagcttta gcgcctatct
     2161 tagcacattc caaagttggg tctggttcta cttcaaagaa tgtagtgtgc ttaatattca
     2221 tttcatctaa taaatcagta actgctttag tatatccatt attgtaaagg aatgtatctg
     2281 ttacaataaa agctctcttc ttattcatta catctttaag ttcttttaat gctactccta
     2341 aacaaccttt tttgaaataa actttttctg gtgctctaaa ccaaagcata ttttctctcc
     2401 tctcagctac tgttttaata ttaagtaaat gcttaactcc tacattttca gatactgaat
     2461 ttccacccca agacccacat cctaaagtaa gagaaggagc tagtttaaaa ttatataagt
     2521 cccctatacc accttgagaa gaaggagtat taattaaaat tctacaagtt ttcatggctg
     2581 aagtaaactt atcaagtttt tctctttgat ttacatcatc aatatataat gaagatgtat
     2641 gcccaaatcc accatcttct actaattttt ctgcttttct aactgcatca tcaaaatcac
     2701 ttgccttgta cattgcaagt actggtgaaa gtttctcatg agcaaaagct tcttctattt
     2761 caactgattc tacttcacct attaaaacct tagtatctac tgggacttca aagcctgcaa
     2821 gttttgctat agtacaagct gtttgcccaa ctattttaga atttaatcct ccatttacta
     2881 aaattatatt tcttattttt tctgtttcat ctttatctaa taaataagca ccacgctctt
     2941 taaattcttt tttgacttca ttgtatatat tttctaatac tataaccgat tgttcagaag
     3001 cacatatcat accattgtca aaagtcttag atacaagaat tgagtttaca gcagttttta
     3061 tatctgcact atcatcaatt attgctggag tattacctgc accaactcca atagctggtt
     3121 ttccagatga atacgctgat ttaaccattc caggacctcc tgtagcaagg gtcaaatcca
     3181 cttctttcat aacagttgta gttaattcta aagatggctc atctatccat cctattatac
     3241 cttcaggagc tccagctaaa acagcagcct caagaacaac ttttgcagcc tctatagtag
     3301 aattttttgc tcttggatgt ggagagaaaa ttattccatt tctagttttt aaagctaaaa
     3361 gtgctttaaa gatagcagta gaagttggat tagttgtagg tacaactgct gcaataacac
     3421 ctattggttc agcaatttta gtcataccaa atgcttcgtc tttctctata acaccacaag
     3481 ttttagtttc tttatatgca ttatatatat actctgaggc ataatgattc tttataacct
     3541 tatcttcagc aattcccata ccagtttctt tttgtgccat tatagctaga ggaactcttt
     3601 gcttattagc tgccaaagat gctgctaaaa aaattttatc tacctgctct tgactatatg
     3661 tagcaaatat tttttgagct tctctgattt ttgctagttt tttaactaag ctttcaacgt
     3721 tatttacagt atctaaacca actgacataa cttcattttc ttttttattt tctattatta
     3781 cttgttcttt atgagccatt ttaacaacct cctgagatag ttttttattt attatgttaa
     3841 tttattaaca ttatagcatt tacttttcct attttcaagt tattttttta acattttatt
     3901 ttttatctaa tataacatca tttctactaa taatatctag ctttacaaat acttaattta
     3961 actagatatc actttatttt ttacgcaaat cactaatgaa
//
LOCUS       DAEMIK010000234         3948 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEMIK010000234 DAEMIK010000000
VERSION     DAEMIK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.238"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   76..831
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=145..172
                     /rpt_unit_seq="tttacattccatatagttaatataaaac"
     gene            complement(1490..3583)
                     /locus_tag="KPK87_003670"
     CDS             complement(1490..3583)
                     /locus_tag="KPK87_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2539772.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPEEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVEIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1420 a          591 c          381 g         1556 t
ORIGIN      
        1 gtaattttat tatatttttg tatgctaata taactagata atcataagat aacacattaa
       61 aagaaaatta taaaaactta cattctgtat agttaatata aaacataagt aatataaatt
      121 tatgtaaagc aataaaagat ggatttacat tccatatagt taatataaaa cgatttcaaa
      181 tgattagaat aaaaatatca gaattgctat ttatattcca tatagttaat ataaaacttt
      241 aaatcatctt tatgaacttc aaaagtagga agcgtattta cattctatat agttaatata
      301 aaactagtaa aatcaaacta tgaaagaata aatgttttta atatttacat tccatatagt
      361 taatataaaa catcaatact ttgatgcagt ttttaactag tttttcaatt tacattccat
      421 atagttaata taaaactgat aagattcttg ttactgctgt tcgaaagttc aaatttacat
      481 tccatatagt taatataaaa cgtttgagga tttattagca actcatagtg aaaagtatat
      541 ttacattcca tatagttaat ataaaacatg gtgtcaaaag gctatattaa cacctaggta
      601 caaaatttac attccatata gttaatataa aacttagtgt ttgtgataat ttcagtttct
      661 tttaaaaatt ctttacattc catatagtta atataaaact agttttaata aaaacgaatc
      721 ttttatttgc aagcacattt acattccata tagttaatat aaaacgaggt ggttggtatg
      781 gtggaggaag cggtccaacc ggatttacat tccatatagt taatataaaa caccaaaata
      841 aacttagtat tttcaacact tacacgtata caactctctt aaatttgcag tgaacgagca
      901 atagtgcaat tgataacgct tatcatgcac tctcaacgcc ttatatttca actgttacgc
      961 caaatttttt cacaaatatc gctcactgca aaatttctat aattttatta tatcataaat
     1021 atattacttt tgaatattga taccaattta taccataata aaagcaaaaa aacgtaatct
     1081 atctacatta gatttaaatt ctataactta aagtttttta cataatattt atgcttgttt
     1141 aatcaatcta atatatcatt tttgtatatt gcttttaatg gtcttttttc ttaaataaat
     1201 atctatctga acaagcatag tttaattaaa aacttttgta ttcccttaaa actgtatcta
     1261 cctcttatta ttttatttat gatatattat ttttaatcta aaaatagata tctattttta
     1321 ttatggttat ccctttttaa aatactttaa tcatatacta ttcccaattt aacatccatt
     1381 gaaatgctat tttcttatat tgcaatttat aattgatata tcttcaatat aataatttaa
     1441 tgtatttttg attttaaagc ttttcagtaa tattattgat aaattagaat taattatcta
     1501 gctttaaaag gtcaaagatt tctgttgctg tctgcggttt tccaaataaa tatccttgta
     1561 tgtaatctgg ctttatttgt ttaactaaat catactcttc ttgtgtttct acaccttcta
     1621 aacatacttt tatttctacg tcatgacaaa ttgcaacaat aaaatgtata aatgtagcat
     1681 caaacttact ctttaatata tctttaacaa atactctatc aattttcact atatctattg
     1741 gagaaaattt taaaacttct agagacgagt atcccgtacc aaaatcatcc atagcaatat
     1801 aaattcctaa atcttgtaaa gccttaaatt taaactgtaa aatactcata ttttgtactg
     1861 tatgactttc tgtcaattct aaaacaagat tttgataagg aaaattttca tcatctataa
     1921 tttttactat atcttcaata aatgtgtctt ctaatatttg aacagctgaa acgtttacac
     1981 tcactttaaa aaaaggatag taatcaatcc atttcctaaa agtacgtaat gccattcgaa
     2041 gcacccaagc ccctacaata tttatcatat cattttcttc taatattgga ataaattcca
     2101 aaggagaaat tgctttgcat ttgtcattag tccaccttaa aagaacttct acacctatta
     2161 ttttatgact ttctgtatct acttgtggct ggaatcgaag actaaacccc ctaaaatcat
     2221 cattaattga tgcctttaaa tcccgcatca tttctaatga atacatttta ttttttaata
     2281 tttcttggga gaaaaaaaca agcctgtttt taccatgctc tttcgcatat tgtaaagagt
     2341 aactagcaca cttataaagt tcttttactg tatctccatg ttttggatat attacacaac
     2401 cagcagaaat tgttatattt aatccatatt ttctccatag ttgaaggtgt ataatcatat
     2461 tctgtatttg attatataga gttagaattt cattttcttc aacattatca acaagaatgc
     2521 ccatttcgtc cccatcaagt ttataaagtt cagcattatc tggtaaaata gattgtatag
     2581 actgtgctag tgtttttatt atattatctc caaaatccct atcatacatc tcattaacat
     2641 ttttaaaatc gtcaatatct aataatacta tgcataattt ttcaatcata ggatttgaaa
     2701 ttttatcttc aaatacactc ataaattcat gataattcag taactgtgtc aatgaatcaa
     2761 tcttattttg tttacctaat tgcgtcatta ttcctgcaaa aatacttggt tctccaaatt
     2821 catcacgcat tagttgacct ctacatctta accatatata ttctccacta cgatttttag
     2881 ccctaaattc cactgtatgg taatccatct ggtttttacc tatttcagtg tttgacttat
     2941 aaaagcgatt ccagtcttct ggatgtacaa tttttttcca gtaaacaagc ggattttcaa
     3001 caatttcttc tggtaagtca aataattcaa cttgtgatgg tgaatatcga aatacgcctg
     3061 ttttcatatt gcaaatgtat atatattcat ctgtacctct aatcaaagca tcataaagca
     3121 atgtagaatt ataatcaaaa ttgtctcttg aaaaattaaa aactcctttt tcttctttat
     3181 tatttgatat ttcatgaaat tcattaatct taattggttt atttaagctt gttgagtttg
     3241 caatatctat tgattgatgc atatgaacaa ctgtgccatc ttgccatgta attaaactat
     3301 cataattttc aaaaacacga tttaacttat tacatttttc ataccacttt attaatactc
     3361 cttttttatc attctttaat aattctaaaa ctttacaaaa actacaagta gaattttttt
     3421 caggatataa tacttgccaa caaacttttc cttctggatc taaaatatta tattcttctt
     3481 tcattttttt gttcataaaa ataatttcat tagtatgaat atttgtaaca tatatattaa
     3541 tctgtagttg atttaatata acttcaaaat tatgtttgtt catatactta tctcctccat
     3601 agttactgct ctttataaca acattgttaa aattattctt tttagaagat ttacttataa
     3661 cttatacata tacactaaat accttaattt aattagaaag tttattactt catattttaa
     3721 attataattg ataaaaaaaa atacagcaat atagtattac caatttaaaa aaaattaata
     3781 ttacataata catctctctc ttatattaaa ttttaacttg gtactaatct tgacaaattg
     3841 tccacaaaaa taaattatag attttattat tgttttaaat ttttataaaa aatcagtatt
     3901 attgatacta ttgaagttaa aaagcattaa tttctaaaaa catcataa
//
LOCUS       DAEMIK010000235         3913 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEMIK010000235 DAEMIK010000000
VERSION     DAEMIK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.239"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(307..507)
                     /locus_tag="KPK87_003671"
     CDS             complement(307..507)
                     /locus_tag="KPK87_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2539773.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(732..908)
                     /locus_tag="KPK87_003672"
     CDS             complement(732..908)
                     /locus_tag="KPK87_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539774.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(962..1051)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1231..1431)
                     /locus_tag="KPK87_003673"
     CDS             complement(1231..1431)
                     /locus_tag="KPK87_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539775.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1433..1783)
                     /locus_tag="KPK87_003674"
     CDS             complement(1433..1783)
                     /locus_tag="KPK87_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG2539776.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1945..2133
                     /locus_tag="KPK87_003675"
     CDS             1945..2133
                     /locus_tag="KPK87_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2539777.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYNHSQVERWVNI
                     LGECALKIKKFSILYGSS"
     gene            complement(2610..2780)
                     /locus_tag="KPK87_003676"
     CDS             complement(2610..2780)
                     /locus_tag="KPK87_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539778.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3169..3657)
                     /locus_tag="KPK87_003677"
     CDS             complement(3169..3657)
                     /locus_tag="KPK87_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2539779.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
BASE COUNT         1512 a          511 c          434 g         1456 t
ORIGIN      
        1 acaaattact aaaagtgtta tatcagaatt gaatacaaaa gaaagagtat gtatttatga
       61 gatagttgaa aattttggtg tgccacaact aaaattatta tattactata tattctaatt
      121 aaaagagtac ttgtaggttt aagtacatac tttataaata tatttttgtc agatttattt
      181 tataaaaagt atttagacta agtattattt ttaagtattt tcttgttgaa gttatttttt
      241 ccttttttac aaaatgcgta aataaaaaaa agactcttaa aagagtcttt ccgaaaaatc
      301 tatatattat aatatagtta tattttctgc ttgaggacct ctagcgcctt taactatatc
      361 aaagcttact ttttgacctt cttctaatga tttaaatcct gaagtttgta tagctgagaa
      421 atgagcaaat acgtcatctc cactttccat tgatataaat ccaaatcctt tttcattatt
      481 aaaccatttt actattccat tattcattaa aaatactcct attaacctat atcaagatat
      541 acttgattta cttgtgtatt accacaaata ctattgtatc atagattaac ataaatgtct
      601 atttaattat aatttttagt taacattttt atttattctt aatatgaaaa taaatttaaa
      661 cattttatat taccaatata ttaatgtaaa tgtagataaa actaaaaagg gttttctaga
      721 aaaaaatctg tctaattata cttacttacc aataccatca cttgatgcaa gcaagattcc
      781 acaacttttt ctccgtactc taatataaaa tcatctaact gtgtatctat aaaatcacaa
      841 taatcatatg aactaccaat ataagcagtt aacatatgtg tacatttatc cattttatct
      901 ctaaacataa tgtatttatc cccctaaaaa actttcatga aaaacaaaaa gaaaatatct
      961 ttttggcaac tggctgacat aacttatttt ataagcgtta gtccctatag ctttgcgtca
     1021 ctaaatttct ctagttttgc cgatttagtt ttattctaca gattaataat acaatagaat
     1081 tagttattat tcaacatatc tagctgaaaa attaataaat ttatatatga acgttaaatt
     1141 atctgaaaaa ataaaaagcc cctctatcag gagtgactta aaaattgaat ttaagtttgt
     1201 atttaaaaca catttttaat aatgcaaaaa ctaatcaaaa ataaacaatt catcaatagg
     1261 tgtatctact atttttgata tatctattgc taatttcaaa gaaggattat actttcctgc
     1321 ttctaaacgc ataatggttt ctcttctaac tcctactttt tctgctaatt gttcttgagt
     1381 taattctttt aattgtctat atttttttag gttacaaatg aatttgggca tattattttt
     1441 cctctaaaat ataaagtttt atagcataaa catttaaagt gattgcatat gtaattgata
     1501 gtaaaacaca cattaattct atattcctag acactatagt aatacaccat aaaatgaata
     1561 attctataat agcaagctgt cctgtaaatg ccatagctgc tttttcatct tgtacatacc
     1621 tttcatctgc tttatcacca tttatttttg ctataataaa gtttgagaaa aaagcaaaaa
     1681 atccaatata tgtaaggtca gcaacattgc cagatgaaaa atatctaaga cttaaaaatc
     1741 caaggaaacc acaaaaaccc ataaaaaatt ttttctgaat cattttttat cctccttagt
     1801 aattttaaaa gagatatatt tatatcttta tgatacaaat atatctcttt tatattcttt
     1861 tgtcaataga aaatcagtat tatacaatat ttgcactgca gcataattgt tagagcaaaa
     1921 atctactgct tgagatgttt atgtatgact aaatatgatt ttaatttcaa attaaaagta
     1981 gctaaatttt atttgaatag agaaggaaga gatattttaa tagtaaaaca atatggagtt
     2041 tataaccatt ctcaagttga aagatgggtt aatatattag gagagtgtgc actaaaaata
     2101 aaaaaatttt ctatactata tggttcaagt tagatgttat aaactatatg atgataacta
     2161 aatatagtgt acaataaatc gctaagtatt ttggaataaa caatagcgca gttatagtaa
     2221 catgaaaata aaaattttta catagtggtg ttgatgcctt ttccaaaatg aaaggaagac
     2281 cataatgaat aaaaacaaaa acactaaaga aaaaataaat taacaagaga acaagaatta
     2341 gaatatgaaa ataaattttt aaaagatgag cttgagtttt taaaaaagat gtgtgtctca
     2401 gggaagccac attaaaaaac agtggtatca ttattaatca aaagaaagtt agatgtatta
     2461 tcaaaaaatt agaacttaaa tgcatagcat tttttcataa atcacataaa tataactcct
     2521 ataaaggtat aattggaaaa gtaaaaaata ataaaacaaa tagaagattt aaaacatcaa
     2581 tagcatatca gaaaataaca aatgatatgc tagaatttat aaaatatatc taatgataaa
     2641 aatggaaaat caattataaa aaaggcatat ctcgattcat tttttgttat atagtcaaat
     2701 aagaatacta tagatgaaag atacgatttt gcatatgtat taagtaagta ttctgatgtt
     2761 tttggttgat acacatgcaa taaagaagtt gtgtttgcag ataaattagc aaaactatag
     2821 tataagttgc aacaataaag aaaatgaaaa ttatcatact aaagcataag gcatgttaca
     2881 attttattag ataggacatt aacatacact aaagtacttt ttattataaa gagtacttat
     2941 ttttttgaaa ttcattaatg tataaactat caagaatatt actcaacata atttaaaaac
     3001 aacttgaata ggtattttta aattatgtga tataataaaa aatagaagtg tatgccaata
     3061 cacttctgta gttataacta agcattctat tcttgaggag tgcttttttc atttttataa
     3121 ccaactcttt ttactttaac aataattatc agacctatat atttattttt aattgtctga
     3181 tttattagat aaaaaagtta ttatcatatt tttatcattt ggtattttta ttgaactacc
     3241 ataatcttct tctaatttat ctagaccact tttacttatt tctccatcta taattatgtt
     3301 taattctaag tattcttcac taccattaga tgacactaaa gtgtacgaac cattataagg
     3361 catgcctaaa ttttctattt tatgtgccaa agggtcttca tcaccaatac cagagtttac
     3421 tgttattttt ccatcttttt tagtccacca tattatgttt ccttcagtat attttgattc
     3481 atctgcatag tagctaagag acgcactttt tattgcactt atatcagctt caagtttagc
     3541 tatctttgct ttttctatat ttttaaataa tgctggaagt gctactattg ccaatattcc
     3601 tattatagca attactacta ataattccac tagagtaaat ccttttttat ttttcattta
     3661 tttcccctcc atataaaatt aataatatat aaatttttat tttgttgatt tctactttct
     3721 atattttacc atatatagac aaaaaaatca ctttctatga caaatatact attattttac
     3781 aatttatgga aatgatttaa aatgacattt cttgttagaa aagatataag aaatggatta
     3841 aaataaagaa aactacagtc atatcaatgt caataatatt atttaaaagc agatgaaaat
     3901 aaaaagagga ctc
//
LOCUS       DAEMIK010000236         3780 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEMIK010000236 DAEMIK010000000
VERSION     DAEMIK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.240"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(178..558)
                     /locus_tag="KPK87_003678"
     CDS             complement(178..558)
                     /locus_tag="KPK87_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2539780.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(759..1952)
                     /locus_tag="KPK87_003679"
     CDS             complement(759..1952)
                     /locus_tag="KPK87_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG2539781.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2015..3271)
                     /locus_tag="KPK87_003680"
     CDS             complement(2015..3271)
                     /locus_tag="KPK87_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG2539782.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1368 a          701 c          405 g         1306 t
ORIGIN      
        1 tatttttaaa aagtaattta agtattagaa gtaattgaag ttagtaaata taaatgcgtt
       61 tacttttaag tattgcttaa gggaataaaa aataaaaata aactctgcaa ccatctaaaa
      121 atagttgcag agcttacaaa accaaaatta tctaaattgt aataaaaata ataacattta
      181 tacataagct tcttcccaat ttactaccca gttttctaac gactttatct tttcctctgt
      241 tagctcctct tcgtgaaatg atactaataa attccttata aaattattct tcaaaagatt
      301 ttgcacattt cttgatataa actctaaata tgccttttct tttattgcaa ctgtataata
      361 catacacttg cctgtttctt gaacatataa gaatcttttc tttgatagtt ttgataaagt
      421 ttttaaagct actttttctg gtaaattatg ttcttctttc atataatcaa taatctcata
      481 tgaattggtc tttatatcta aattccaaat aaatttcata ataactaact ctaattttga
      541 taattttttt aaacacatag ttatcactcc gcttctttat ctatataatt aaatattaca
      601 ataataattt cacaaaatca attctaataa tcataacata ccttaaaatc tgttcttaac
      661 ttctaccttc ttaatctccc aaatgatttt tttatattgt attcaatagt ataatccttt
      721 tccaaaatat aacttaccag ttaaaataat ctttattttt acttactctc gccttttaca
      781 gaagttaata attttttata agttacatca attccaaaag ctccaagagg tgcagttata
      841 agtatagcga gaactgctaa cgtcaaaata agttgtccag atgccatccc cattgcaagt
      901 ggtacacctc ctattgctgc ctgtactgta gcctttggaa tatagccaat catacagaac
      961 aacctttcct ttattgtcaa atttgtctta ataaggcaga agaatactcc taccatacgg
     1021 aataaaagaa ctccaaatat taaaattaca gctccaattc ctgctttaaa tgcataactt
     1081 atatctactg ttgcccctac caaaacaaac aagagaattt cagcaccaac ccatagtttc
     1141 gaatacttta tagacaatct tcttgcaact acacgatatt ttatctgaag gaaaatccca
     1201 atactcataa ctgctaataa tccagaaaat ggcacaatac cctctaaagc agtttctaaa
     1261 cttatcatta aaaatgagat acttaaaagc aacacccctt tagcagaatc tctcatatga
     1321 accttcttaa aaaatactgc taaacagatt ccaatcactg ctccagctat tacacctaat
     1381 ataattgata ctggaatttg taaaaaacta atagctgata tactatttcc ttgtgccaat
     1441 cctgtgaata ctgtaaacat cacaattacg aaaatatcgt ctatagaagt tcctgctaaa
     1501 agcatctgtg gaatactttt ttctgtacca taaccttctt ccattaattt aagcatttta
     1561 ggtacaataa ttgcgggaga aactgctcct actactgcac ccatgactgc tgcttccaat
     1621 atagaaacgc ctagtaattt tggtgctaaa actatcattc cgataatttc aaaagtagct
     1681 ggaataaagc acataagtac agcaggtctt cccactttct tcaaatcgtt tatatctaaa
     1741 gataatccag cccttgttaa tataataatc aatgcaatct ttctaaggtc agaagaaata
     1801 tctagtattg aattatcaat caaatttaaa acatatggac ctaatataat tcctgtaagt
     1861 aacattccca ataagttagg taattttatc ctcttaaaaa tacctcccga ggccattcct
     1921 aataaaaaaa tcaaagctaa acttgtcaac atattcctac cctatccttt ctctaatatt
     1981 ctttctcttc taatcataca ttatgttgtt ttatttattg ttttgttgaa attttttgta
     2041 gtgaataaat agatttatcc atattaaata tatatgaaat agcgcatact acaaagaaat
     2101 aaggtaaata gctgtaccca aaaacttctg cccctataaa tacaggtgca aaaaaagtat
     2161 ttgtagcact accaaagaca ctagcatagc caagcgctgc aactaattca attggcaaat
     2221 taaaaaatcc tgctaacaat acaccaagac tagctccaat ggaaaacaaa ggtgtaactt
     2281 ctcctccttg aaaacctgct gacaaagtca gtattgtaag tataaacttt aacagccagt
     2341 caaatgaata aatttctcca ccatagaaac tattttgaat taaatttgtc cctaaacctg
     2401 aatatcttcc tttataaaat aaaaggaata atacactaag acatactcca ataatagcaa
     2461 tccttatcat aggatttttt aatctattac ctatttttct ttttgacaat ttcagacacc
     2521 atgcaaatgc tccaccaacc attccaaaaa taattcctaa aacaataagc ttccaaaata
     2581 ttgacaagtc aaatacaact ttagaagaaa gtgcaaaaga aaatttctct aatcctaaag
     2641 cttttgatac tgaacttgct gtaaaagaag cagtaaacgc aggaaaaagc gattgatact
     2701 ccagtgaacc agctaccaaa acctccatag caaatagtat tgctgctatt ggtgtctcaa
     2761 ataatcctgc aaaaccagct gccataccag ttactagaaa aatactagaa gcatttttta
     2821 taggcaacct tttacctacc caatgtgaaa aagttgcacc aatctgaaca gcaactcctt
     2881 ctcgtcctgc actaccacca aataaatggg ttaaccatgt acctgatatt ataaatggaa
     2941 ccaatcttaa aggaataatt tcttcttctc catgccctac ctcaaatatt agattcattc
     3001 ctttactact ttttcctcca aatttaaaat agcaataagc aataacaact cctactactg
     3061 ctaaaaatgg tatcagatac attggatatg tttctcggat atctgtaact tttagaagaa
     3121 ccgttccaaa tatggtgtct ataagaccta taattgcacc aactggtata cctatgagtc
     3181 ctaaaaaaaa tagtccacca tatgttttat aaacgctttt aattcttatt atcattttaa
     3241 tccatattaa agttttaaat ttttctatca ttttaattcc tttctatttg catcataaga
     3301 ttttttgaaa gttttattgc atcaactgta gtcaatgtaa tgactatcgt aattgttttt
     3361 taaattgata ggatataggt acactcttaa cccattctta ataaaataat taacatataa
     3421 tctactattt tttctattga ttaaatattt cttcaaattt aaaaaataaa aatttagtaa
     3481 gtaattcacc tccattttat cgatttaaac aacaaaaaaa gctggtggca tctatgatat
     3541 catagaagtc atcagctcat tggcagttta ggatattatc atccaaggga gaacctcatt
     3601 cccataatta ttatataatt ttttattttt catatgtcta ttttatgtaa attgtatttt
     3661 taatgttata aataatataa cctatcaatt aaaatattaa agtctctaat attatgagtg
     3721 ttcaatattt catattattt ttatgatata taatttaaat gattttcttt actttgtatt
//
LOCUS       DAEMIK010000237         3766 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEMIK010000237 DAEMIK010000000
VERSION     DAEMIK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.241"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            318..695
                     /locus_tag="KPK87_003681"
     CDS             318..695
                     /locus_tag="KPK87_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2539783.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            688..1836
                     /locus_tag="KPK87_003682"
     CDS             688..1836
                     /locus_tag="KPK87_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG2539784.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVRNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1853..2455
                     /locus_tag="KPK87_003683"
     CDS             1853..2455
                     /locus_tag="KPK87_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG2539785.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2548..3570)
                     /locus_tag="KPK87_003684"
     CDS             complement(2548..3570)
                     /locus_tag="KPK87_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2539786.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNISKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNLKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT         1473 a          491 c          535 g         1267 t
ORIGIN      
        1 attagaaggt tattaaaatt tattagatat tactctagtt tattttaaaa aaaagtatta
       61 taatattcct tgtgtgaaaa tataggaatt tttattaaac agttttaatg tgtaaataac
      121 attctacact attgtaatta aatatgtaaa aagtaaaata tttaaataga tatattttaa
      181 aatctaactt ttaatattgt aaaaaaatta gaaatttaac aaaaagtaca aaaattcaca
      241 tttttttcat aaagtgtttg actaaaaaat tcacatgttg tagaattaag gtagcaggag
      301 ttttgaggga ggtgtaaata atgataagaa aaataccaca agcagaacta aaagtaatga
      361 aattcatttg gaaagtagat gttacagtga catcaaaaga tgttattgaa gctatggaac
      421 aaaaatatgg ctggaaacaa accaccacac tgacactttt atctaggctg gtaaaaagag
      481 gattcttaga tgctcaaaaa atagacagat atacacatta cacaataaga gtaaaacaca
      541 aagattattt aagttttgag acaaaggatt tcctaagtaa tatacatgat gactctttaa
      601 gaagcttaat ttcagcatta catgatgatg aaaatatagg taaagataag ctagattttc
      661 tagaaagtta ttttgataat ttaaaggatg aataattata tgctatatgt ttttgaaaat
      721 cttataaaga caactattgt atgtagtctt ggtatttgtc tattattatt tttgaaaaga
      781 tatttattta agaagtttag taagagattc aattattata tttggttgat aatagtcttt
      841 agaatgttac tttttctatt taattataca atagtatatg aagtaaaaga acccaaagaa
      901 aatgcagtaa gaaataatat tacacaaatt gatatttcta cagataataa tcttatgctg
      961 tatgtagctt atttatggtt atttgtaact atagtcattg cagtttatac atttataaag
     1021 tatacaagat ttaaaaatct agttgttgat gtatcttatg atattgaaga taatgatata
     1081 aactgtatct acaaaaactt gttaaaagaa ttaaatataa aaaagaaaat tgaattaaga
     1141 ggttctgatg aattgataag tccagcaggt atgggattat ttaaatctta catattctta
     1201 ccagactatc cttatagtaa agatgaacta acttggattt tgaaacatga attaatgcat
     1261 tttaaaaata aagatatttt aataaagttt ttagttttgt ctgttagaat aatatattgg
     1321 tttaatccac ttgtatatgt aatgagcaat aaagtaaatc tagattgtga attatgttgt
     1381 gatgaaagtg ttttggtaga atgttcttta aaagataaaa aagaatatgc actagcactt
     1441 ataaagtcaa ttaagtttag taaaaattat aatagtaaga ttttgacaac cgagtttgat
     1501 aagacaaatt tggaaaagag acttgagagt attgttaaaa agaaaggcaa aagtggaata
     1561 ctaattgctg ttttagtatt tatgacttct ataacatctt ttgttgaagt tgatgctcaa
     1621 gttagaattg atgagggtaa taatggaatg aataacaata ttaatgaatt tttattgaat
     1681 aagacgactg ataaaattat aaatatttat tataaagatg caccaaatga aattaggaaa
     1741 ttttatgaag ataaatgcga attagaaggt aaaataccta gagactatga cataatagaa
     1801 atggatatga aagattataa tcaattaata aaataattat aaggaggatt ttatgttaac
     1861 aaaattaaga aaaatagtag ctattatgtg ttgcttattg cttactttta caagtgtggc
     1921 atttgctgac acaagtaaag agaaaggagg aagtaaagag actgttccag agaaaaacct
     1981 agattctttt actctttctg ggtatatata tacatatgaa aatgcgccac aagaaataag
     2041 agatgaacat aaacaaaatt gtgaagagat aaatatagac cctaagccag atgatgagat
     2101 atttgttcca gaatcagcta atctaatgaa tgaggacaat agtaaaggtg tttatgcaag
     2161 ttatactgtt tcttataata taggtgctaa aactataaca attatgagta atgagtattt
     2221 gactataagt acaacaaaag ttatacgaaa gggtaatagc ggaaaagaag taaaagctgc
     2281 acaaatcatg ttaactttac ttggttataa tgtaggtata gatagttctt ttggaagtaa
     2341 gacatataat gcagttgtaa gtttccaaaa aaaatacggt ttgtctgcag atggaataat
     2401 tggtccagct acttgggata aattgggtag actaactgac cctactcttt cataggatta
     2461 aaatctaaaa atatattaaa tagtgtctca atatgatttt tcaatttaaa tgagacacta
     2521 tttaaatata aatgttataa aactatttta caataaattc catataactc taccatctat
     2581 atccttaaca ttttgacaac cagtcaaaat catagtctca cataactcat tcctaacctt
     2641 ctctataaaa aactccacac catcaagacc accgccaaaa gaagcagtaa caaaaggtct
     2701 tcccatcaaa acagcatcag cacccaatcc aagcatctta actacatcaa caccagtcct
     2761 aactccacca tcaacaagta ttgttatctt tccctttaca gacttagcta tatctggaag
     2821 tacctcacaa gtaccaggag tataatcaag tactcttcca ccatgatttg aaacaataat
     2881 ggcactagca ccagactcta cagccatcaa agcatcatct acagtcataa ttccttttat
     2941 aataaaaggt aatctagtgg attcaaccaa ttctctaatc tcatcaattg ttttaggaga
     3001 aaaaggattt tcttgaaatt gattattaat aagtccacaa gcatcaaggt ctacaccaac
     3061 tgcaaaagca cctgcttctt cagacaatct aattttttct attatcttta aattattcca
     3121 aggctttata aatacaattc catttcctct attatccttc aacacatcta aattatccag
     3181 taaaaaagta tctacattag tatcgccaac catagcataa attccagaat tagaacaacc
     3241 tctaacaaca ggctctatgt attctttttc ggaaacttta ccacccatgt tcaatatagt
     3301 acctgataca ggtgcagcga atataggaga actcattttt ctaccaaata attctatgga
     3361 tgtatctggc tttgaaatat tatgtatgac tctcatattt atctttattt tttctagact
     3421 ttttctattt tcaatgaaag acgaaccact tcctttacca cccataccag gaacatctcc
     3481 agcacaagct aatccattac aaattttaca tactttacat gttccattaa agttctctct
     3541 agctgatttt agtaagtcat tgtaattcat acaaaatctc tcctttttat aatattaaaa
     3601 ttgagtatta ctatattgaa aatatcttaa ttgtaaatat agggatgtta ttatgatgag
     3661 ctaaactaga taatacatat tagatatttt ttattgttgt agatagttaa attatatcat
     3721 attagatagt caaattttga aaaaaactaa cttatttaag tataca
//
LOCUS       DAEMIK010000238         3761 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEMIK010000238 DAEMIK010000000
VERSION     DAEMIK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.242"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            285..1865
                     /locus_tag="KPK87_003685"
     CDS             285..1865
                     /locus_tag="KPK87_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG2539787.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIGMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            2039..2485
                     /locus_tag="KPK87_003686"
     CDS             2039..2485
                     /locus_tag="KPK87_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG2539788.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISSNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2506..3549
                     /locus_tag="KPK87_003687"
     CDS             2506..3549
                     /locus_tag="KPK87_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG2539789.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1530 a          338 c          700 g         1193 t
ORIGIN      
        1 tttttataaa aatgaaaaat ataaataagt cataaaagat tataaaaata aagagatatt
       61 tagataatta gtgtaaatag gttttaaatc aacataataa cttaaattat atggtataat
      121 aaattaaata attgttttaa ttcaaaattt tttgttattt taaattattt caaaactctt
      181 agttttagtg attttattta tgatatttat gggattaaaa agatttcaat agatattttt
      241 ggtaagtatt taagaagtct ttaactttat aggagtgata atttatgcta ataggtacat
      301 ctaaattaag cagaaatgtt gatactgcat gtgtagaaga agtagggatt ccaatgattg
      361 tattgatgga aaatgcggtt atatctgcaa tgaaaaatat ggatataggc atgtataata
      421 gttatacagt tgtatgtggt gttggaaata atggaggaga tggtcttgga atagcaagac
      481 atctaaatac atatggaaaa gaagtaaacg tatttttagt aggtaatctt gaaaaactaa
      541 gtgaatgctc taaaataaat tacaatgtac ttttaaatat gggaattaat attataaata
      601 ttaataaaaa tgatttaagt gaaaaaaatt atggagatga aaatgaagta gataaaatta
      661 ttaaagttga gaaacaaaat attataaaaa ataaaaattt aacaattttt aaagatagag
      721 tatctaaaag tgatgttgta gtagatgcta tatttgggac tggattaaaa cgtgatataa
      781 ctggtatttt taaagaagtt attgacatag taaacgataa tagtaaaaat acatattcta
      841 tagatattcc ttcaggtata gatagcgata atggaaatgt attaggaact tgtattagag
      901 caaacaaaac gattagcttt gagttttata aaagaggttt tctaaactat gacactagca
      961 cacttatagg tgatgtagtt gtagaaaaga taggtgttcc agattttatt acaagaaaat
     1021 accatgatga agagttcata actgataaga cttttgtaaa aaataatatt agaagaagag
     1081 ataagcatgg ttttaaaagt gattttggaa aagtatctat agttgcaggt tcaaaaggat
     1141 tttatggtgc aagtttcata gcaactgaat ctgctgttaa gagtgggagt ggacttgtaa
     1201 cattaattag tagtgaagat gtgcaagaaa aggtatctat tagacttaca gaagcaatga
     1261 ctttaaattt tgaagaagaa agattaaata aattacttaa ttctagtgat gcaattggtt
     1321 ttggtccagg tatgggagat aatagtcaaa catttgataa attacttaag atagtagaaa
     1381 attctaattg cccaatagta ttagatgcag atggattgaa cgttatgaag gacagatgtt
     1441 ataagttttt agaatggaaa aatagatttg taattacacc tcatttggga gaaatggcca
     1501 ggttgacagg agattcaatt gggtatataa gagaacatag agtagatgtt gcaaaagaat
     1561 ttgctcaaaa atataatctg gtggtgcttt taaaaggtta tcaaactgtc ataacagatg
     1621 gtaaaaaaac atatataaat cctacaggaa atagctgtat ggcaactggt ggaatgggtg
     1681 attgtttgct tggaatgatt acttcattta taggtcaagg tatggatata ttggaagcta
     1741 cagttagtgg agcatatata catggctata taggagataa attgagcaaa gcaatgtata
     1801 cggtaaatgc tacggattta ataagtaaca tttcattaac aatgaaagag ttgttggata
     1861 tataattggt gatatttgat actactttgt aacaatttta acaaagtatg ttgatataat
     1921 actattaatt aattaattta ttttattgtt ttgtaattta aatttatatt caaaaacatt
     1981 gtataaacat ataatttata tagggtttta taattcatat gaaaagaggg tagatagtat
     2041 gaagttaata tggttgatag tagcagtctt atttggaata gcagaaatgt taacaccaag
     2101 cttaacattg atatggttta gtgtaggagc tgtaatactt atatttttaa gtagctttat
     2161 agagagtatt ttcttacaaa ttttaatatt tgcagtaatt tctatagcaa tgttagtcgt
     2221 agctactaaa aaaatagtaa aaaaagataa aggctataaa tctaacacta atttacaagc
     2281 aatgatgtct aaaaaaggta ttgttacaga agagatttct tcaaataaca caggtctagt
     2341 tgtagttgaa catgaaacat ggacggctat ttctatagat ggtgaaaaga tagaaaaagg
     2401 ttctacagta gaagtattaa aaattgaagg ggtaaaatta gttgttaaaa aagtagacgc
     2461 cacagttaat gtgacaaacc aatagaataa aggaggattt atagtatggg tactaaaatt
     2521 gttttaagta ttgtattaat agtagtagtc gtagcaataa gtttaacttg tataagagtt
     2581 ataaaacaat ctaaagtagg tataataatg agacttggta agtttcaaaa agtagctgaa
     2641 acaggtgtac atttcttaat accattttta gataaaatgg catatgtaat tgaccttaga
     2701 gaaattgtaa tagattttcc acctcaacca gttataacta aagataatgt aactatgcag
     2761 attgatacag ttgtatatta taaagttaca gacccagtta gatatgtgtt tgagatagct
     2821 aatccaatag ctgctattga aaatttaaca gctacaacac taagaaatat aattggtgaa
     2881 cttgatttag atgaaacatt aacatcaaga gatataataa atgtaaaaat gagaactatc
     2941 cttgatgaag caacagataa atggggtata aaagtaaata gagtagagtt aaaaaatata
     3001 atgcctcctc aggatattca agttgcaatg gaaaaacaaa tgagagcaga aagagaaaga
     3061 agagaggcaa tacttcaagc agaaggtaat aagtcagctg caatacttca agcagaaggt
     3121 gaaaaacaat ctgcaatatt aacagcagaa gccaaaaaag aagctatggt acgtgtagca
     3181 gaaggtgaaa aagaatctgc tatattagtg gcagaaggtg aagctgaagc tataagacag
     3241 acagctattg ctaaggcaca aggtgaagct gaaatgataa aaagaactca aatggcaaca
     3301 gcagaaggtt taaaattagt tttttcagca atgaaagagg ctgatataga caataatatt
     3361 ctagcattaa aatctatgga agctcttgaa aaaatggctg aaggtaagtc aacaaaactg
     3421 gttttacctt cagaagcagt taatttccta ggaacattca aaggaataaa ggaagttatg
     3481 agtgatgata acaaagaagt acttgatata aaagaagttt taaatgataa tgaatcatta
     3541 aaaaaataaa agagttttgt tatcatagga aggatatgga aattaaaggt cgaattaatt
     3601 aaatatagca caaaaatgca cgttgaaaag aagaggactt ataaattttt aagtcttctt
     3661 ctttgttttt attagaaaga tgaaattata aaaataaatt aacagaatct ttatacaatt
     3721 tatcctttac taatgtaaaa agtgttatat aatataatat a
//
LOCUS       DAEMIK010000239         3732 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEMIK010000239 DAEMIK010000000
VERSION     DAEMIK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.243"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(182..3580)
                     /gene="cbpA"
                     /locus_tag="KPK87_003688"
     CDS             complement(182..3580)
                     /gene="cbpA"
                     /locus_tag="KPK87_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG2539790.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAGGDANAGNAEEDSNAGNAEGDANAGNAEEDANAGNAEED
                     SNAGNAEEDMNALVVEEATAEDPTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQ
                     NNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSE
                     NKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKK
                     GDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYG
                     AGIGGGGNGSGGDITINGGTITAKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYS
                     GNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLS
                     ATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDD
                     FKMLIDGKIYPYGAPSYTDDQGVLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFY
                     VKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFEEDVEKLFLDWILKDGGIPIIT
                     PKGEKLTDKKFMTISSQRLNEETMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYP
                     FNEAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPD
                     CASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTI
                     TVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDW
                     KPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVD
                     DYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDS
                     GNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDG
                     KPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPDINIDTDGDGKPDINIDTDE
                     SLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         1058 a          802 c          409 g         1463 t
ORIGIN      
        1 atgatatcaa ttccattaat ctcttttaat aaaaaattct cttttctata tgaaaattat
       61 ttcaaaactt ttaagttaaa tacaaaaatg actataaata gatttttatt tacacaaatc
      121 tatttatagt cactatagtt tatccaataa aataataaaa taaatatgta cacaataaat
      181 gttatttacg tcttaagtat ttatttgtta gatttttaat taaaaaaaac aagctaatga
      241 acattaatgc taaatctagc attatattac tttgatctcc tgtttgtaca ttttgaccta
      301 cattactatc taaactttca tctgtatcta tatttatatc cggctttcca tccccatctg
      361 tatctatatt tatatccggt tttccatctc catctatatc tatatttata tctggttttc
      421 catctccatc tatatctata tttatatctg gttttccatc tccatctgta tctatattta
      481 tatctggttt tccatctcca tctgtgtcta tatttatatt cggttttcca tccccatctg
      541 tatctatatc tggtttgcta tttcctccat tatctgggtc tggtttacta tttccaccgt
      601 tatctggatc cggtttgcta tttcctccat tacctgaatc tggtttgcta tttcctccat
      661 tacctgaatc cggtttgcta tttcctccat tacctgaatc tggtttgcta tttcctccat
      721 tatctggatc cggcttgcta tttcctccat tatctggatc tggcttattt agatttggat
      781 tacctgtatc ataacctcct gagccagtat ctacattttt atcaggcttc caataatcat
      841 ctactccatc attatctgta tctattgctg gacgattata atctgtatca gggtttccat
      901 ctccattggt atctatattt ttatcaggtt tccaaatacc atctccattt ccaccttctg
      961 tactaggctt ccattcacct gtattatcag tatctatgtt aatatctggc ttccaatcac
     1021 cactcgtatc tatattgata tctggccttc catcaccatc agtgtctata tttatatccg
     1081 gtattccatc cccatctaca tctacatttg tcatatctaa ctttaacttc ttttcatcct
     1141 cactaccttc aatgtaattt atatcatctc caatatattt tacagtaata gtttgttcag
     1201 tagtaaaaat atgatgattg ctatctaaag gtctccattc aatagatgca gtactataat
     1261 tatgactctc actaccctta ttatgagttt ttaattctac tggatcttta tattcctttc
     1321 cattaatata gaattgtacg taacctcttg gagaggcaca atctggtcct tctttcttat
     1381 ctggtgaaat agtaacattt aatgtaaacg tatctgttgg tttaaaaaca tcagttttag
     1441 ctttagactc ttctacaact aatgaagttt ctgtatcagc aggaataatt tctgccttat
     1501 aatatgctct atgcccccaa taagcttcat taaaaggata tacatttgaa aactgtgttg
     1561 atgtaatgct taattgatat tttccagcat caacttgctt tgtaaggtca tcacttccta
     1621 aatttgtatc tggttccatt gtttcctcat ttaaacgttg agaactaata gtcataaatt
     1681 ttttatctgt gagtttttca cctttaggag taattattgg aataccccca tcttttaata
     1741 tccaatctaa aaataatttt tctacatctt cttcaaatgg taatccatca tatcttttaa
     1801 taatcaaata tggtggagta tcatcaatag taaattcttc atattgcttt aaaaattgag
     1861 aacctgaacc tatatcttta acataaaaat catctggttt tatatttcct ccagtaattt
     1921 tatctataac aactaaatta acagatactt cagatccctt aaattgatca gaaaaccata
     1981 agtaaagaac cccttgatca tctgtataac taggtgctcc ataaggataa atttttccat
     2041 caattagcat tttaaaatca tcaactaatg tagtactaac tttatcttgt cctaaatact
     2101 ttaaagatat tttattcata aaaacttttg ttttcttttc taaatcaccg tatgcaactg
     2161 ctccacctaa acttttgaat gttgttgctt ttaatgaacc acctgttact ataacatctc
     2221 ctgttgtagc acttaggtca aaaatccaat ctcccactcc tgtcatataa ggtaaaatcg
     2281 ttcctccagt tacaagtata cgatatcctc tatttgttcc tggattaaca catccatccc
     2341 caaatgcact accatgtgca cctccatatg aataggaaag tccaccatta attgtaatat
     2401 ttccactata accagaacct gtgctaggat ctactactga gggacttaat gcattctttg
     2461 ttgttgtata gtatcctcca cctattccag ctccatggta tgcaccttta gcagttatag
     2521 taccaccatt gattgtgata tctccaccac taccattacc gcctccacct attccagctc
     2581 catatcctgt attacgactc gattcttgac ctatagcagt tatagcacca ccattaataa
     2641 taatatttcc tccactttca gtatttccac cgccaatacc tgcagaataa taatttccaa
     2701 caacttttaa acttcctcta ttattactat ctagtagtgt aaggcgatct ccttttttta
     2761 acttgtctcc attttctaga gttacatctc taggaatttt tccaccctca ggaactataa
     2821 actctccttc aatatctcta tttacaacag aatcatcaat aattagagta gtaccttttc
     2881 ctgctctcat acctggataa tatacatcac ctgactctag cttgttttca ctaccatctt
     2941 gtaatataac atgtgcatat gcaccaccat ctttatctgg tgttaaagta aatgggctat
     3001 tagatgcagt tttaatattt actccatcaa atattaattc agcttttaca tctttaggta
     3061 taacaatcct atgtgttgtt ggagtatttg gattattatt ttgaattgtc atttgttttc
     3121 cactaagaat agttaaaacg ccacttgcaa atttaaaatc gacatttatt tctccaccac
     3181 taattttaaa gtctcctaca gatgtaggat cttctgcagt ggcttcttcc acaactaatg
     3241 cattcatatc ttcttctgca tttcctgcat tagaatcttc ttctgcattt cctgcattag
     3301 catcttcttc tgcatttcct gcattagcat ctccttctgc atttcctgca ttagaatctt
     3361 cttctgcatt tcctgcatta gcatcccctc ctgcatttcc tgcattagta tctccttctg
     3421 catttcctgc attagtatct ccttctgcat ttcctgcatt agtactttct tctatagtat
     3481 ctgcgaaagt atttgacgca ttaaatgatg ctactgatcc aagtcccatt gagatactta
     3541 aaatcattga tagtgctttc tttttacatt tttgttgcat aaattccccc tccccttaat
     3601 aatttttaat atacactgta tacatcctta tatacactct aataattctt aatatacact
     3661 gtatacatcc ttatatgcac tctaataatt tttaatatat acttaaaact tatatttgtc
     3721 aatgaatata ct
//
LOCUS       DAEMIK010000240         3729 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEMIK010000240 DAEMIK010000000
VERSION     DAEMIK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.244"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..3612
                     /gene="tcdA"
                     /locus_tag="KPK87_003689"
                     /gene_synonym="toxA"
     CDS             <1..3612
                     /gene="tcdA"
                     /locus_tag="KPK87_003689"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG2539791.1"
                     /translation="FIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVK
                     VNGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFT
                     LVGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDI
                     STSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKK
                     VNINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFK
                     DIKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLF
                     YFDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGP
                     DGFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNNSKAVTGWRIINNEKYYFN
                     PNNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGK
                     HFYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNN
                     SKAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYY
                     FNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAIL
                     YQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSY
                     DGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQ
                     NKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNT
                     AEAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYF
                     APANTDANNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVA
                     VTGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPA
                     NTDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANG
                     YKTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKI
                     YYFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         1399 a          446 c          589 g         1295 t
ORIGIN      
        1 tttattgctg aagatataaa tgtatttatg aaagatgata ttaatactat aacaggaaaa
       61 tactatgttg ataataatac tgataaaagt atagatttct ctatttcttt agttagtaaa
      121 aatcaagtaa aagtaaatgg attatattta aatgaatccg tatactcatc ttaccttgat
      181 tttgtgaaaa attcagatgg acaccataat acttctaatt ttatgaattt atttttggac
      241 aatataagtt tctggaaatt gtttgggttt gaaaatataa attttgtaat cgataaatac
      301 tttacccttg ttggtaaaac taatcttgga tatgtagaat ttatttgtga caataataaa
      361 aatatagata tatattttgg tgaatggaaa acatcgtcat ctaaaagcac tatatttagc
      421 ggaaatggta gaaatgttgt agtagagcct atatataatc ctgatacggg tgaagatata
      481 tctacttcac tagatttttc ctatgaacct ctctatggaa tagatagata tataaataaa
      541 gtattgatag cacctgattt atatacaagt ttaataaata ttaataccaa ttattattca
      601 aatgagtact accctgagat tatagttctt aacccaaata cattccacaa aaaagtaaat
      661 ataaatttag atagttcttc ttttgagtat aaatggtcta cagaaggaag tgactttatt
      721 ttagttagat acttagaaga aagtaataaa aaaatattac aaaaaataag aatcaaaggt
      781 atcttatcta atactcaatc atttaataaa atgagtatag attttaaaga tattaaaaaa
      841 ctatcattag gatatataat gagtaatttt aaatcattta attctgaaaa tgaattagat
      901 agagatcatt taggatttaa aataatagat aataaaactt attactatga tgaagatagt
      961 aaattagtta aaggattaat caatataaat aattcattat tctattttga tcctatagaa
     1021 tttaacttag taactggatg gcaaactatc aatggtaaaa aatattattt tgatataaat
     1081 actggagcag ctttaactag ttataaaatt attaatggta aacactttta ttttaataat
     1141 gatggtgtga tgcagttggg agtatttaaa ggacctgatg gatttgaata ttttgcacct
     1201 gccaatactc aaaataataa catagaaggt caggctatag tttatcaaag taaattctta
     1261 actttgaatg gcaaaaaata ttattttgat aataactcaa aagcagtcac tggatggaga
     1321 attattaaca atgagaaata ttactttaat cctaataatg ctattgctgc agtcggattg
     1381 caagtaattg acaataataa gtattatttc aatcctgaca ctgctatcat ctcaaaaggt
     1441 tggcagactg ttaatggtag tagatactac tttgatactg ataccgctat tgcctttaat
     1501 ggttataaaa ctattgatgg taaacacttt tattttgata gtgattgtgt agtgaaaata
     1561 ggtgtgttta gtacctctaa tggatttgaa tattttgcac ctgctaatac ttataataat
     1621 aacatagaag gtcaggctat agtttatcaa agtaaattct taactttgaa tggtaaaaaa
     1681 tattactttg ataataactc aaaagcagtt accggatggc aaactattga tagtaaaaaa
     1741 tattacttta atactaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa
     1801 aaatattact ttaatactaa cactgctgaa gcagctactg gatggcaaac tattgatggt
     1861 aaaaaatatt actttaatac taacactgct atagcttcaa ctggttatac aattattaat
     1921 ggtaaacatt tttattttaa tactgatggt attatgcaga taggagtgtt taaaggacct
     1981 aatggatttg aatattttgc acctgctaat acggatgcta acaacataga aggtcaagct
     2041 atactttacc aaaatgaatt cttaactttg aatggtaaaa aatattactt tggtagtgac
     2101 tcaaaagcag ttactggatg gagaattatt aacaataaga aatattactt taatcctaat
     2161 aatgctattg ctgcaattca tctatgcact ataaataatg acaagtatta ctttagttat
     2221 gatggaattc ttcaaaatgg atatattact attgaaagaa ataatttcta ttttgatgct
     2281 aataatgaat ctaaaatggt aacaggagta tttaaaggac ctaatggatt tgagtatttt
     2341 gcacctgcta atactcacaa taataacata gaaggtcagg ctatagttta ccagaacaaa
     2401 ttcttaactt taaatggcaa aaaatattat tttgataatg actcaaaagc agttactgga
     2461 tggcaaacca ttgatggtaa aaaatattac tttaatctta acactgctga agcagctact
     2521 ggatggcaaa ctattgatgg taaaaaatat tactttaatc ttaacactgc tgaagcagct
     2581 actggatggc aaactattga tggtaaaaaa tattacttta atactaacac tttcatagcc
     2641 tcaactggtt atacaagtat taatggtaaa catttttatt ttaatactga tggtattatg
     2701 cagataggag tgtttaaagg acctaatgga tttgaatact ttgcacctgc taatacggat
     2761 gctaacaaca tagaaggtca agctatactt taccaaaata aattcttaac tttgaatggt
     2821 aaaaaatatt actttggtag tgactcaaaa gcagttaccg gattgcgaac tattgatggt
     2881 aaaaaatatt actttaatac taacactgct gttgcagtta ctggatggca aactattaat
     2941 ggtaaaaaat actactttaa tactaacact tctatagctt caactggtta tacaattatt
     3001 agtggtaaac atttttattt taatactgat ggtattatgc agataggagt gtttaaagga
     3061 cctgatggat ttgaatactt tgcacctgct aatacagatg ctaacaatat agaaggtcaa
     3121 gctatacgtt atcaaaatag attcctatat ttacatgaca atatatatta ttttggtaat
     3181 aattcaaaag cggctactgg ttgggtaact attgatggta atagatatta cttcgagcct
     3241 aatacagcta tgggtgcgaa tggttataaa actattgata ataaaaattt ttactttaga
     3301 aatggtttac ctcagatagg agtgtttaaa gggtctaatg gatttgaata ctttgcacct
     3361 gctaatacgg atgctaacaa tatagaaggt caagctatac gttatcaaaa tagattccta
     3421 catttacttg gaaaaatata ttactttggt aataattcaa aagcagttac tggatggcaa
     3481 actattaatg gtaaagtata ttactttatg cctgatactg ctatggctgc agctggtgga
     3541 cttttcgaga ttgatggtgt tatatatttc tttggtgttg atggagtaaa agcccctggg
     3601 atatatggct aaaatatatg tttgataaaa aattattcct gtgctactaa gaaattattt
     3661 ttatataata aatattgaga tttaattaaa agtcatgtgt tattgtaata catgactttt
     3721 agttaaaat
//
LOCUS       DAEMIK010000241         3695 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEMIK010000241 DAEMIK010000000
VERSION     DAEMIK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.245"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            470..1372
                     /locus_tag="KPK87_003690"
     CDS             470..1372
                     /locus_tag="KPK87_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539792.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKYKASTKNKSEKRGVVDVTN"
     gene            1359..2114
                     /locus_tag="KPK87_003691"
     CDS             1359..2114
                     /locus_tag="KPK87_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2539793.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
     gene            2211..3248
                     /locus_tag="KPK87_003692"
     CDS             2211..3248
                     /locus_tag="KPK87_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2539794.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNSTKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
BASE COUNT         1378 a          478 c          556 g         1283 t
ORIGIN      
        1 ttatttaaac tcaattttct gataatttga taatttttca aaatttatag ttgactttta
       61 ttttttactg tattaaacta attactatac aaaacagaaa acaaaatttt caaaattatt
      121 aaattaaaag gagaggtaaa tatgaagact ttattaaaca aacaagtacg attttttagt
      181 agccaattta gttagggttt gtacctaagt taagacaaat tgtcataaca tagatacaag
      241 ccccttagga ttatgatttt taaggattgt atctatgttg gctatagtaa ttgttgttaa
      301 agtttttatt ttatcatcat aatatagagc cacatggatt atactgtgtg gcttattcga
      361 atttttgcca tacagttata tatactgtgt ggttttttta tatcaaaatc attttttaaa
      421 atcaaattta aaaaaccatt taaaatattt tcaaaggggt gttttattta tgtcaggtat
      481 tatatctgta atgacgcaaa gtttaatttt atcgattatg gcactggggg tttatataac
      541 ttacaaaatc ttagattttc cagatatgtc tgctgatggt agctatacaa tgggtgcttc
      601 tattgtagca ttttctctta ctaatggtat ttctcctgta gtagcaactt taatggctat
      661 cttatgtggt tgtactgctg gtcttgttac agggattctt cacattaagt ttaagatatc
      721 taatttactt tctggtattt tagttatggg aatgctatac tctatcaatt taagaataat
      781 gggaaaatca aatattcctc tatttagctt taaacactta ttcaatgggg aaatatctcc
      841 tatagtttta gccttagctt ttgtattcat ctgtaaagtc cttttagatt tattcctaaa
      901 aacaggtctt ggatacactt taaaaggtgt tggtgataac tctcaaatga ttaaatctct
      961 aggtataaat ataggttcta taaaaatact aggacttatg atttcaaatg gacttatagc
     1021 tttatctggt agtcttatgg ctcagttctt gggattctca gatgtaaata tgggtatagg
     1081 aactttagtt cttggaattg cctctatcat aattggtatt acactattta agaaatttac
     1141 ttttatcaaa gatactactg caataattgt tggctctttt atataccaat tcactatata
     1201 ctttgcaatg agtttaggaa tgttatcaac tgacttaaaa ctaataactg ccatagttat
     1261 aatagctttc ctagctactg gtaacttaaa tatttcacta aaaaaaaaca aatacaaagc
     1321 tagtaccaaa aataaatcag aaaaaagagg tgttgtagat gttacaaatt aaaaatcttt
     1381 caaagagttt ccctaatcca tatggtgaac ctaatactat ctttgaaaat ttatcaatcg
     1441 atatagaaga tggtgaattt gtaagtatca taggtagtaa tggaactggt aaatctactt
     1501 tattaaatat aatatctggg cttataaaag aatcttctgg tcaaattact ttagatagta
     1561 ctactataac aaatttagct gagtataaaa gaactcaaat agtaagtaga gtttttcaag
     1621 acccaagcct tggaacttgt ccttctatga cggttagaga aaatttatct ctagcactta
     1681 ataaagggaa attattgaac ttaaaaaaat gtcttcgtca taaaacgaat gacttggaac
     1741 acttacttga aggaatttct ttggatttaa aaaaatattt agatattaaa gtacaatatc
     1801 tttcaggagg tcaaagacaa tcactatcac taattatgtc tagcttagca agtcctaagg
     1861 tcttattact agacgaacat acagcagcac ttgacccaaa aacatcaaat gaagtaatag
     1921 agttaacaga taaaatcgta agagaaaaaa atataactac acttatggtt actcacaact
     1981 tgaaacacgc tcttcaatat ggagataggt tgataatgct tcataagggt gaagttgtat
     2041 tagatgtaaa agataaagaa aaagaagatt taacagttga agaaatctta gaaaaatttg
     2101 aatatgcagt ataagaaatt aaaatcttag ttttaatcag aaatatttat aatctatcat
     2161 atgaatatat gaacaaatat aattaataaa ttttaaggga ggaaaataaa atgataaaaa
     2221 gtaaaaaaat attaagttta attatagcag gagtacttgg tgtatcaatg cttactggat
     2281 gttctcaaaa tgatggctcc aatgcttcaa atgaaaataa agaaacagat agcaaaaaac
     2341 aaaaaaatat aggtattagt caattagtgg aacatccatc tctggataaa gcaaagaaag
     2401 gattcatcaa agcactcgaa gataaaggct ataaagatgg agataatata aaaatagatt
     2461 tccaaaatgc acaaaatgat atgcctacta cacaaagtat tgctagtaag tttgtatccg
     2521 acaaaaaaga tttgatatat gctatatcta ctccatctgc acaagcagct tataattcta
     2581 ctaaagatat tccgataata atgactgctg ttacagaccc tgtagaagct ggacttgtta
     2641 aatctcttga aaaaccaggt ggaaatgttt ctggtacatc tgattatctt tcaattgata
     2701 aaacattaga attggttaaa acattgactc caaaagcaaa gaaaataggg gttatataca
     2761 atactagtga agttaattca aaaatccaag ttgattctct acatgattat gctaagaaaa
     2821 ataattatga agtagttgaa aaaggaatca gctcttcaag tgaagttaac caagctattt
     2881 ctagtttagt tggcaaaata gatgttttat atgtccctac tgacaattta atagtttctt
     2941 ctatgccaat agtttctaaa gttgctaatg aaaacaagat tcctataata gcctctgaag
     3001 aaggttctgt atcttctggt gctttagctt gttgtggaat agattatgaa aaactaggtt
     3061 ataaggctgg agaacttgct attgaagtat tagaaggaaa atctgttggc gatataccag
     3121 ttactacatt agatgaaact gaaataataa ttaacgaaga tacactaaaa gcacttgaca
     3181 tgcaaaagtt atcagcagat aatataaagt atataaagtc agatgaaaat gcaaaatctg
     3241 caaaataaaa cctattaatt tcaaaaaata ggtaatatga agtaggtaat atgaaaactt
     3301 tatataataa gtactcttat ccaccctaac cctttgaaaa ttgtgcttat tgtagcaatt
     3361 tgaatattgc atataaagtt attcattttc cataaaagtc gtctatattg acgactgatt
     3421 atgaaagtta tataactttc taatcactac ttgagctgtc caaaagaatt ttaatattct
     3481 ccagacagct tttttttatg atatttgatt gatatacatg tctagttatt ccatatttag
     3541 taaatatttt gataaaattt ccacattcac ttataactta ggataacgtt tttttcaaac
     3601 aatacattaa tttaaaagta ttctgagtat acattattta gtattttaat taaatttagc
     3661 agttgacttt tattattgta taatttataa tttat
//
LOCUS       DAEMIK010000242         3668 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEMIK010000242 DAEMIK010000000
VERSION     DAEMIK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.246"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            310..1686
                     /locus_tag="KPK87_003693"
     CDS             310..1686
                     /locus_tag="KPK87_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG2539795.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1997..2668
                     /locus_tag="KPK87_003694"
     CDS             1997..2668
                     /locus_tag="KPK87_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG2539796.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2857..3654
                     /locus_tag="KPK87_003695"
     CDS             2857..3654
                     /locus_tag="KPK87_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG2539797.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDINIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1391 a          407 c          679 g         1191 t
ORIGIN      
        1 taacttcaaa aaagtatatg aaatttatac tggtaaaatg ataaataaat tttaagtaca
       61 aaatagtaaa agctatgata gttttagacc tattatatgt aaaaaatata gatgtttttg
      121 gtgcaattta aattaaaaga tatatataaa aatttaaaat attccaaaca gaaattataa
      181 aaattactat cattgtcttt atacaatatt tatactataa tagaataata atttagagga
      241 aagctctaaa aataaacaaa ttaagggtct taacagtagt gattttatgt taaggaggta
      301 taaagaaagt tgaaaacaat agttagaaga cttattctct acgcagatgg aatgcgtcct
      361 actcaaataa tggtatctgg gtttgctgcg attattgtaa tcggagcatt gttgttaaca
      421 ctcccgatag cgtcgcagag tggagagagt ataggcttat taaatgctct atttacggct
      481 acttcagcgg tttgtgttac tggtctagtt atggtagata cagcaactta ttggagttta
      541 tttggacaga tagttataat aacattgata caaattggtg gtcttggatt tatgaccgta
      601 gccacaatgt tttcacttat ggcacgaaaa aaaatacaac taagagaaag attattaata
      661 caagagtcat taaatcaagc tgatttatcg ggacttgtaa gacttacaag atttgtatta
      721 ataataacca taacaataga gggaataggt gcactggttt tatcaactgt ctttattcca
      781 caatttggtt tgtctaaagg gatatggtat agtgtatttc acgcaatatc agcgttttgt
      841 aatgccggat ttgacttgat gggcagtgta agtggcccat ttacatcgtt aaattcttat
      901 gtaaacaact ttacagtatc aatgactgtt tgcgcactta tagtgcttgg agggctagga
      961 ttccctgttg tacttgatat tgtaagaaaa agaagatttt caaaattaaa tgtgcattct
     1021 aaggttgtat tgttttcaac tgctacactt atttttgtgg gtgcattatt tatttttctt
     1081 atagaattta atcaaaaagc tactatggcg gacttgcctt taaagggaaa agtactatca
     1141 gcaatatttc aatcagtaac agcaagaact gctggtttta atacgcttga tttagctact
     1201 ttacgagaaa gtagtgtatt tgtaatgata attttgatgt ttattggagc atcacctgca
     1261 tcaactggtg ggggtataaa aacaacaaca ctagctgttt taatcattac agttagaagt
     1321 tttctatcag gaaaatcaga tatagaagct tttgaaagaa gattagctcc atcaactata
     1381 aaaaaatctc ttggtatatt tgtaatcagt atatcagcag ttatttttgg aacattgatt
     1441 atttcaataa ctcagcctaa ttttacatta gttcaatcag cttttgaggt tacatcagca
     1501 cttgctacag ttggttcaag tttagctgga actccaaact taaatgcttt aggtaagatt
     1561 ataataataa tctttatgtt tatgggtaga gttggttctc ttactttatt tatggctata
     1621 ctttctggtg gtagaagaaa gagtcaacca atcagatatg cagaaggtaa aattatggtt
     1681 ggttaagtta ccaaggagta atataaattt ataatggatt tagagtagta ctgtaaatct
     1741 gttgataaaa taaatgagcc tagatgtaat tatatgaaga aataaaatcg actaatgaac
     1801 aaaattaatt aatgaaataa aaaaacaggg aaataaaata caaataaaaa ggtaaatcaa
     1861 aattaaatta atctgtgtat ataaaattat atattgtgta taagttttgt atgtgtaaag
     1921 tatataagtt ttgtatgtgt aaacaattaa tttaagagat attatataaa ttgtgtatta
     1981 gggaggaagt ttcgagatga agcaatatat agtcattggg tgtgggagat ttggaagttc
     2041 agttgcgtct actatgcatc ttttaggaca tcaagtaatg gcaatagaca aaaatgaaga
     2101 ttcagttcaa agtatatctg acaaggtaac ccattcactt atagtggatg ttactgatga
     2161 gcaagcgtta aggtcattag gtttaggtaa ctttgatgta gcagtagttg caataggttc
     2221 tgatataagg gcatctataa tggcgactct tatagccaaa gaaatgggtg tagagttgat
     2281 aatatgtaag gcaaaggatg aattacaagc taaagttctt tataaaattg gcgcagatag
     2341 agttgtattt ccagaaagag atatgggagt aagagttgca cacaatttag tttcggataa
     2401 tatattagac catattgaac ttgacccaga gtattcaatt gttgaaatcg taactccaaa
     2461 tagttgggtt ggcaagacac ttatagagct tgaattaaga gctagatatg agataactgt
     2521 acttgctata aaaacaggta aaaatataaa tgttacacct tctccagatg aggaacttac
     2581 agccggaagt atcctagtta taatcggtca aaatactagt ataacagcga taacatctgg
     2641 aaataagggg ataattagaa gaagataatt tactatttaa tatatattta attgcaatga
     2701 aagtagatag tatcatataa ttatgaatag ttatatgata ctattttttt aatcgaaggt
     2761 agtatttatt tgtaagatta gataaagaga agttaaatta aaagtaagga ggctgtgcta
     2821 atataaaaat ttataagtta ttagcattta gataaaatga ttacaaatat aaacagtaag
     2881 gataatgaaa agttaaagta tacaagagca ctattaaaat caaagaatag gaataaagag
     2941 tcaaagttca taatagaggg atacagaata gtaatgcttg cacttgaatg tatggcaaac
     3001 cttgattatg tatttatcaa tgaagaattt gaaaataaga aagaacatgt aaaactatta
     3061 gaagatttgg ataaaaaaaa cacaaagata tacaagacta ctaataaaaa ctttaaagaa
     3121 ttagtggata cagaaaatac tcaaggaata ataggtgtag tttcatttaa gaaaaaaaaa
     3181 ttaagtgaaa gtataaataa aaaagataaa tttgtattga ttttagacag aatacaagac
     3241 ccaggaaata tggggactat aataaggact gctgattctg ctggagtaga tgccataata
     3301 gcactaaagg gatgtgtcga tatatacaac ccaaaagtaa ttaggtctac tatgggttct
     3361 atttttgata ttaatataat tgatgcttca caagatgaaa ctgtggacat gcttaaatca
     3421 ttggatttta atatagtttc aagttactta aatacagaaa atttttatga caaaatagat
     3481 tatggctcaa aagtagcatt ggtgatagga aacgaagcaa atggaataaa tgaagaactt
     3541 gtatcaaagt ctgatatttt ggttaagata cctatatatg gtaaagccga gtcgttaaat
     3601 gctgcgataa gctctgctat actgatgtat gaaataaaaa aatacttaat ttaatgtatt
     3661 gtaataaa
//
LOCUS       DAEMIK010000243         3651 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEMIK010000243 DAEMIK010000000
VERSION     DAEMIK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.247"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            4..1551
                     /locus_tag="KPK87_003696"
     CDS             4..1551
                     /locus_tag="KPK87_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2539798.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIGVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKVIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMKISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1554..1952
                     /locus_tag="KPK87_003697"
     CDS             1554..1952
                     /locus_tag="KPK87_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG2539799.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1992..>3651
                     /locus_tag="KPK87_003698"
     CDS             1992..>3651
                     /locus_tag="KPK87_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG2539800.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKTNLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNK"
BASE COUNT         1539 a          298 c          603 g         1211 t
ORIGIN      
        1 tatatgaata aaaatagtat gacaaaaatt tacagtttaa ataaaataat ttttattata
       61 attggaattt taggaatagt tttacttaca agacatattg gggtagatat taaagtttta
      121 attttaattt tattaacctt atttagtata aatgtattta tttcatatct taaaattaag
      181 ttgtatgagg ataaaattaa tttaggattg ataaataatt cagataagtt tataaatatt
      241 actaaagatg agaacttcct aaaacatgtt caaaattata ttatcagtaa tgaaaaagaa
      301 aactgtgtaa tggcttgttt tgatttatgt agactcaaac ttataaatga tatatatggt
      361 tatgagttag gggatgaagt gttaaataat attttaagta acttaaaaaa ttattttgga
      421 aaagaagcta tttatggaaa gttaaaaagt gatgtttttg tattaataat aaagcttgaa
      481 aatagggaac agaagattcc ttatttagta aatcttatta agaataaaat tgtgatacta
      541 agtcagattg acagttttaa aatggatata aatattgaag cttctattgg aatttataaa
      601 tttaataata atgaaattga tataaaaaaa gttatagata atgctgatat ggcaaggctt
      661 aaatcaaaag gactaaagca catagagtat gtagaatttg ataatgctat ggaagaagaa
      721 atacaatcaa ttatgaaaat agagcgagat ttattcttgg ctattaaaaa taaagagttt
      781 gtaattcact atcaacctaa agttgattca agtacaggta acattatagg ctcagaggca
      841 ttaataagat ggttgcaccc atcattaggc atggttggac caaataagtt tatacctata
      901 gctgaaaaaa atggtctgat aaacaatatt ggtagatggc taattcaaga agtttttatt
      961 accattaaca agtggatttc agaaggtata aatgtcttac cagtctcaat aaatctttct
     1021 agagtagaac tatataaaaa tgatttagtt gattttttta aattgatgtt taatacatat
     1081 aatattccaa aagaacttat agaattagaa attacagaaa cgacagcact aagagatgta
     1141 aaatttataa gtgaaaggct ttatgagatt aaatctttag gaatgaagat atcattagat
     1201 gattttggag taggtaattc aaattttatc aaccttaagg ggataccact agatattata
     1261 aaaattgata ggtcacttat attggatata gttacaaata caaaaacaaa gtttatggtg
     1321 aaagcaattg ttaacctttc acatgattta aacgtaactg ttatatgtga aggggtagag
     1381 gatatgcatc aggtcaaagt cttaagtgaa ctgggatgta atttcataca aggttatgta
     1441 ttttttaaac ctttggatga gatgaattat aaaaaacttc ttactgacgg aagttatata
     1501 aatctgaaag atactctttt agataaatgc atgacagttg aggaggagta aggatgattt
     1561 cagaagatgt agattattca ataagaatca taagagctat atttaataat ggagaagata
     1621 aaccaataga tgctaaaatg atattaaaaa gtgaagatat accaatacta acactataca
     1681 gactgcttag aaagctttgt gcaaacaata ttcttacttt taacaaagaa gatggcaaga
     1741 catacatgtt aaatgtgtca ttggatacaa taagtctaag aactattgta gaagtaattg
     1801 atggaaaaat atatataaat aaatgtacga gatatgtatc taattgcagt aattgcttaa
     1861 aaggttgtaa agtaaatcgt gaattgctag aaatagaaaa agaaatttat aaacttttat
     1921 cgagaaaatc tataagacaa atactagaat aaaaaatttt cagctatgat aataattgga
     1981 ggttttcggt tatggatgat atatctcaag ataattttct actgtctaaa gagtatgaga
     2041 atagcttaga tgtagatact aaaaaagcaa gtggaatata ttatactcct aaaataattg
     2101 tagattatat tgtaaaaaaa actttaaaaa atcacgatat aattaaaaat ccatatccac
     2161 gaatacttga tatttcctgt gggtgtggaa attttctttt agaagtatat gatattttat
     2221 atgacttatt tgaggaaaat atatatgaat tgaaaaagaa gtatgatgaa aattattgga
     2281 ctgttgataa catacaccgt catatattaa actattgtat atatggtgct gatattgatg
     2341 aaaaagctat aagtatatta aaagatagct taacaaataa aaaggtagta aatgatttag
     2401 atgaatctga tataaagact aatctatttt gttgtgatag tcttaaaaag aaatggcgat
     2461 ataagtttga ttacatagtg ggtaatccac cctatattgg tcataaaaaa cttgaaaaga
     2521 aatataaaaa atttctccta gaaaaatata gtgaagttta taaggataaa gctgacttat
     2581 atttttgttt ttataaaaag attatagata ttttaaagca aggagggatt ggcagtgtta
     2641 taactccaag atatttttta gaaagtctat caggtaaaga tttaagagaa tatattaaat
     2701 caaatgtcaa tgttcaagaa atagtggatt ttttaggtgc aaatatattt aaaaatatag
     2761 gggtatctag ttgtattcta acttttgata agaaaaaaac aaaagagaca tatatagatg
     2821 tatttaaaat caaaaatgag gatatatgta taaataaatt tgaaacattg gaagaacttc
     2881 taaagagtag caaatttgaa cattttaata taaatcagag attgctatct gacgaatgga
     2941 tacttgtaaa taaagatgat gaaacttttt ataataagat acaagaaaaa tgtaaatata
     3001 gcttggaaga tatagcaata agttttcaag gaataattac agggtgtgat aaagcgttta
     3061 ttttaagtaa agatgatgta aagttgaacc ttgtagatga taaattttta aaatgttgga
     3121 taaaaagtaa gaatataaat aaatatatag tagataaaag tgaatatagg ctaatatact
     3181 caaatgatat agataatgag aatacaaata aaaggatact agatgaaatt atagggctat
     3241 ataaaacaaa attagaaaac agaagagaat gtaaaagtgg aataaggaaa tggtatgaac
     3301 ttcaatgggg tagagaaaag cttttctttg aaagaaaaaa gataatgtat ccttataaat
     3361 caaatgaaaa tagatttgct atagattatg ataacaattt ttcaagtgca gatgtatatt
     3421 ctttttttat aaaagaagag tatttagata aattttcata tgaatatcta gttggaattt
     3481 taaattccag tgtttatgat aaatacttta aaataactgc taaaaaaatg agtaaaaata
     3541 tatatgatta ctatcctaat aaggttatga agataaggat ttttagggat aataattatg
     3601 aagagataga gaatttatca aagcaaataa tatcaatttt attaaataag t
//
LOCUS       DAEMIK010000244         3497 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEMIK010000244 DAEMIK010000000
VERSION     DAEMIK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.249"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            643..1395
                     /locus_tag="KPK87_003699"
     CDS             643..1395
                     /locus_tag="KPK87_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539801.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1385..3496
                     /locus_tag="KPK87_003700"
     CDS             1385..3496
                     /locus_tag="KPK87_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2539802.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKRKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVINKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         1319 a          507 c          463 g         1208 t
ORIGIN      
        1 caacatttta ttattttaac ttctttacta taatatagta atattttaat atctcaaaat
       61 catatcaatc acttttcagc atagtctaaa atacttccac tttctaaaat tatctatcat
      121 acctatacta acaatacttt ttaacctttt tattatacat ttcaattgtt tttaacaacc
      181 tatttatttc caaaactcca atatattttc tcaattacat tgtgttaata acccctaaat
      241 cctttcaaat tatttacaat acattccatt aaataatctt actgctctat aattcttttt
      301 ttctttttaa ccctactata cattatttac atcatttttt tcaatacaac attcgttatt
      361 ctataacctt tttactttta ctatatacct tttttctaac tacaccttca atgttccaca
      421 tcatattact ttagcacaac aattaatacc taaaaataac cctctaaatc ctccaataat
      481 ataatcaatt taactataat aataacctct cacactatcc aaacaatata ttttcaaaca
      541 ttacaaaaaa acatccaact attacaatat atctcatatc cctcctcccc tttcaaatgc
      601 tatcattgac ttacaagata aatgaaagtg aggatgatat atatgcttaa agtaaaccaa
      661 ttatgtaaat catataaaac aggtagtaaa acatatcctg tattaaaaaa tatttctttt
      721 gaaatcaaca aaggagaatt tgtcgcaatc atgggttcat caggaagtgg aaaaactact
      781 cttttaaatt gtatttcatg ttttatccca attgataatg gggaaataat tcttggaaat
      841 aataatataa gaaatttaaa agaaaaggaa ctttcagaag ttagaaacca acaactagga
      901 tttgtattcc aagatttcat gctatttgat ggtcttagtg tatttgaaaa tatatgtgtt
      961 ccacaaatta tcaaaggtag tcaagtctta caaatggaaa accgtgcaat taatctatgt
     1021 gagctatttg gtattgaaca tatcagaaaa aaataccctg ccgaaatatc tggtggagaa
     1081 aaacagcgta cagcagtagc aagagcactc atgaacaacc cagatttaat attagcagat
     1141 gaaccaactg gtaaccttga tagtaagtct tgtcgtacag tcattgaatc atttttaaaa
     1201 gcaaagcaag aattagatgc tactatactc atggttacac acgatagctt tgcagcaagc
     1261 ttctgtgacc gagttattgt gcttaaagat ggaataattt ataaagaagt tgtaaaatca
     1321 aattcaagac ataaattcct agatgaatta ctagaagttt taaaagaaat gggaggcgct
     1381 caagatgaca attaataaat tatacagtaa acttcgagcc tataattgga agaactatac
     1441 tactttattc ttttgtataa tactttctgt ggtattagta acttcatatg ctctaattta
     1501 ttttagccca acaatacaaa acatacttcc aactggtgga gatagcagaa aacaggctac
     1561 aatgatattc tcagttgcta tattaggatg cgctgtcttt actacatacg cttcttccct
     1621 ctttttaaaa tttaaaagca aggaaattgg tattttcatg gctctaggaa cagataagag
     1681 gaaattaaaa aaacttctat tttcagagat attgttaatt tcaatagtat gctgtttttt
     1741 aggtcttata ttaagtattc ctgtatcttt tggtatatgg aaaatatttc aattagtgat
     1801 tattgatacc agagaaatga catatcattt tggaactcaa ggattcatat atggatttat
     1861 atttagtgta tttgtgacat tatgcatatt tatactttct gtaaaattta ttaatcgtac
     1921 aaatataatt gatatattaa atgaccagag aatatctgaa ccaatacagg atgtaaagga
     1981 ctggtatggt atattaggta taatttttat tatactaggt atttttctag gatatctcgt
     2041 accttcacta actataaaca aattaaatta tagactacca tctgtatgga gctttactta
     2101 tctactctct cttattggaa tatatatgat tatgacatac atagttgtac atgctaaaag
     2161 aggaaaacat ccagagaaat actataaaaa catcatatca actagcatga tgagatttat
     2221 gggaagacaa acagtaaaaa acatgtgtgt tatttcattt ttgattgcag gagcattatt
     2281 tgctgtattc tatgttccta atatggttac aggtatctat gatactctta aaaataaccc
     2341 tatagattat acttttacct acaatcaaag agataaacag gtatcaaaaa aagatattga
     2401 acaattagca gataaacaca atataaaaat tcaaaactat atggaaattc cttctataga
     2461 attaattgtt gatggtgtag aatcaatata tcataaagat ggaactatcg aaaaggtata
     2521 tatgaaaaaa agctcctatg atatgttctt taattccaaa gatttttcca agctaattgg
     2581 caaaccaata actataaaaa atggtgaata tcttgctgtt ataaataaag gaaatgaaaa
     2641 tcaagattat aaatatacca aatttatatc aagcccagtc acagaaaaag aaatcccctt
     2701 tagatatgct ggaaaaataa tatttaatgg ttcttttttt caaaatagaa gtatgaacgc
     2761 ctatgtcctt aatgaggagg attataaatc atttactaaa gatttatcca taaaaaatta
     2821 caataattct atactattta atgtagataa agatacctat gcttttgcca atgacttgaa
     2881 gaaagaaatt ataaaaagaa catctaagga aatgggcatg ttcagtggtt atgatgaata
     2941 cgtaaaaaaa cgttatgaat caactggaag aaaatatttt atggatgaag aatatccaag
     3001 tggtgaaggt catttaaaac tagacccaaa caacaatcag ctgtatctta attggaaata
     3061 ctatcctaac tttaaaattt tacaaagcca agatatgata aaaaacatgg cagtcttctt
     3121 aatgttattc atatatgtgg caatcatatg ttttacagca gtaggaatca ttgcatatac
     3181 aaggggtgtt actatagcta taaatcacaa gcaggtcttt atggatttaa atcgattagg
     3241 agcaaacaat gattatataa agttctgtat tagaaatcaa ttaaagaaag tattttcata
     3301 ccctcttatt gcaggaagtt ttttaattta tctgtttaat tttatgatta tgttaaataa
     3361 tgatggtaga atcgtcaata gtgaggtcaa tgctcttttg ataaacttag gttttatagg
     3421 gatttgtggg gtttatatgt accttatata tctattaact tataagaaat tcaaaaaaat
     3481 tattggtatt ttataat
//
LOCUS       DAEMIK010000245         3438 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEMIK010000245 DAEMIK010000000
VERSION     DAEMIK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.250"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..216)
                     /locus_tag="KPK87_003701"
     CDS             complement(<1..216)
                     /locus_tag="KPK87_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG2539803.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSK"
     gene            complement(220..1356)
                     /locus_tag="KPK87_003702"
     CDS             complement(220..1356)
                     /locus_tag="KPK87_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG2539804.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYDTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(1388..1933)
                     /locus_tag="KPK87_003703"
     CDS             complement(1388..1933)
                     /locus_tag="KPK87_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG2539805.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            2257..3036
                     /locus_tag="KPK87_003704"
     CDS             2257..3036
                     /locus_tag="KPK87_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539806.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         1230 a          528 c          382 g         1298 t
ORIGIN      
        1 ttttgaataa ttaatagcaa aaaaactaat caactgaaac attatatttt tagaatatat
       61 taaaattggg atattttcac ctaaagaaat ttgctttcct agttcctcac tataactcaa
      121 tatattttca taagtatatt tttctccatc tataattaaa aaatttttat ttttatgcct
      181 ctcttttttt attcttaaaa gttcataata gttcataatc tacacccttt caagcagtaa
      241 agctgaacct agaccaccag ctgcagctat agagcaaatt ccatatctac ccttagtctt
      301 ttctaatgct tttaaaagat gtaatgcaat aattgcacct gaagcaccat atggatgtcc
      361 atacgctaaa gctccaccaa atatattata tctatctatt aactctggat actttctttg
      421 aaacaacaca tctataactg caaatgcttc attaaattca acagcagata catctaaata
      481 attaagactt tctatctcta aaagtttatc cattgcttgt atcgctgatg ttggacttaa
      541 ggttgcatct gttcctattg tgcatgtatt aataattttt gctttaggct tcttttttac
      601 atgttctaaa tacttcttag aacatattat aataaatgaa gctccatcat taataagaca
      661 tgaatttgct gcatttgtaa tagtttcttt tcctaaaatt gatggcatcc tatctaataa
      721 cctttgactc atcttatcac gtataccttc atcataggta ctattattta taggagatat
      781 aatatcctca agtatttttt cttctcttgc ttcttttgct cttttatgac tttcctttac
      841 ccaaaaatcc aagtctgctt tctcaatttg atatagctct gctactcttt ccgccccttc
      901 gagcatacta ttttggctat tatcatcagg agaaaattga gcaactgtat aatttggatt
      961 atttgtatca tatcgcttat cattcttatg atatgtgcgc attggttgta gagaactact
     1021 ttcaaaacca cctgcaataa ttaaatcaca ctgaccagat ttaacctttg aaaaagctat
     1081 gtctatactc atcatagcag aagcacactg catatccaca gtaaaagcag gtacttcatt
     1141 tgacactcct gctgttaaag tcattaatct agctatattc cctcctgtcc caactgcatt
     1201 tccacatata atctcatcaa ttttgtcaat ttcatacttt ttaattaaat cttttaaaac
     1261 acttgctcct agtaattcag gttgaacaaa ttgaaaaatt ccatttttta gacctatatg
     1321 acttctcaat cctcctaata taaaaacttc ctccatacat tacacccttc cttatattat
     1381 agatttatca tagatttctt ctgagtacat tgttcagttt agtgcctaaa aaagatgcaa
     1441 taaccgactt tattgtatct ccaattaaaa atggtactac tgctgcaaaa agtgctgctt
     1501 ctactgtcat ttgattaaaa tagcacataa atactgttcc cataaaatat ataataggca
     1561 ttccaacaaa aattgttatt agtatatatc tcttaatatc attatttcgt ccttttaata
     1621 aactaataat tagtgctgca aacaaaaacc caaaataaaa accacctgtt gggcttaaca
     1681 actttccaat accagatgta cctcctccaa atactggaat cccaattaaa cctatcatta
     1741 tatatacacc aactgtacta aatgcttgtc ttggtgttaa aattaaagct attaaattta
     1801 tcattattgt ttgtgctgtt actcctgctg gagtaaatgg caatggtata tatatgtaag
     1861 atgatataca caataatgct acacatattg acattttagt taaatcttga atatttactt
     1921 tagtattttt catttttata attcccttca tatgtcttat ttcatttagt ttatatgtac
     1981 atatacaaag cttctattaa agctttgttt tagtataaaa taaagacgtt tttttgtcaa
     2041 ctattataat tatttaggtt tacatttaat ttataagtaa actttatact gttactttat
     2101 attttaatag tcatttataa aactttgctt aaaaacagct ctatttagac tatacttgta
     2161 aattataata tgatatattt ttatataaga ttttatttta tataaaaaat agtaacaaat
     2221 agtttataca caagatattt aaaaggaagt gatgatatga aagttaatga aaataatata
     2281 aataatatag atgaagagtt agatgaactt agtaggaaag tttacttaga gtccccagat
     2341 ttttggataa gtatttctga caaaataata gataaagtat ttgatgaaat gttattattt
     2401 tttgcagtta aatataacta tttcaatata gtatgctttg ctattgaaaa caattatctg
     2461 gatttaaaca tgccttcaaa aaacaaaaaa tacactaata taatggagca tttgttagat
     2521 acatcaaagc agaatgaaga taaaaaaatt tataattatc ttctaagctt aaataaaggt
     2581 aataacctag atgaaagacc aattttagat gaattaaact gtaatattag tagtgatact
     2641 tttgaaaaaa aagatatata tcttccaaag tacttatgtc caagttgtaa ttctaatata
     2701 tttgatacag gttataaagt atcaaatgac ataacatata aattttcttc taaagaagct
     2761 gaacctattg aaatgtgtag tgaagtttct tctatcagat gttgtaattg tgatgaatta
     2821 ttagaagata ttacacttga taaactatat aacctatgca aaattcaaaa ctgtaataat
     2881 tgtggttcta atttaactaa ggtaggaatt attgcaaaga aaaaaatgaa ctttgatgag
     2941 aatactgaaa aatttaaaga tgtctcaaca tcttactgtt gtggaaattg tgactctgaa
     3001 ataaacttat ctcaaagaga acattttaag atttaaatat ttataggcta aagtaataag
     3061 attacattat tagatactgt ataagttttt taaatctgta aaaataaata cacttataag
     3121 aattaaaatt gctatgaaac tagttcttga tgaactaatt tcatggcaat tttctataat
     3181 ctaataatta ttaaatttta acaaaccttt attagcattc cataattatt taaaattctt
     3241 tcaaataccc tatagtttat aggtgaaagt atctttaata ttatatcaaa cttattatct
     3301 ttaaatagta aatttgttaa atctgaaata caccatacaa ttttattttc ctcttttgta
     3361 gctatatcaa ttctgattaa cttacaccta taataaatat ctttatcttc atatagtcta
     3421 ttttaaaaat tactctta
//
LOCUS       DAEMIK010000246         3269 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEMIK010000246 DAEMIK010000000
VERSION     DAEMIK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.251"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2184)
                     /locus_tag="KPK87_003705"
     CDS             complement(<1..2184)
                     /locus_tag="KPK87_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2539807.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKD"
     gene            2350..3093
                     /locus_tag="KPK87_003706"
     CDS             2350..3093
                     /locus_tag="KPK87_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG2539808.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT         1087 a          568 c          337 g         1277 t
ORIGIN      
        1 atctttttca gcatatgtaa cttccatatc tccataatgt ttctttatag attctataat
       61 attaaaatat aatttctcta atgtcatttc tggagttaga gcctttctag ttgcttctat
      121 tacaataggt gtacttacca tatctattgc ttttacttct tttcctgtag catcacgaat
      181 aactttagca aatgttaaaa tagaccccat atcagttaaa attaaaattc cttttccttt
      241 atcaatttta ttggctattt ctacagcttg tactaaaata tcctgaactc tatcttctaa
      301 tcccatatta attgccactg catggttaca atccagtaaa gtattggcta cattcaccat
      361 gctagatgct gtagattcac catgcattat gactaatatt gctatactct cctcatttaa
      421 tcccacatac gataaataaa gaaatgttgc taaaaaaatt gcttcatctt ctggaaactc
      481 tactccatac attaatgaca tgttagataa tatttctttt gctatgttat attcatatgc
      541 tctttccttc ataatttcat ctttattagg atgctttact atatttccaa tctttattct
      601 ttcaattaaa ttctgaatat gtaaagctat agcttttatt aatttatcta atccttctct
      661 tttgctccat ttatcatgct tttgtaaaat atattttaca gtagttgcaa ttgaatcatt
      721 aacaatgttg ttataagctg tttgattact actattatat ataaaagtat tcattaaatt
      781 ataggaatac ttctgtatgt ctccatctat aacacttcta atttgtgact ctttaatacc
      841 ttcttgttga agtttattcc aagtttcttt tatcattcca taaaaatcta aatcatattt
      901 tttattttct attaataaat tttcactttc aaaatccttt tcttctggta aaaaaataat
      961 atcatctttt ccaataaaac tgaaattttg tacaaactcc tcttttctat tatttccata
     1021 taatgattct ctaatattat ttggtagaag agagagcctt acatatacac attccctttt
     1081 ataggtcata taatctaaaa aagctcttgc acaaattaat tgtatatcgc tttttaattg
     1141 gcctatgttt cccttacact tatataaaat aaatgcctta ataacttctt ttgaaacctt
     1201 taacttaaca ccaatcctaa cactctcttc tttaaaaaaa taacatataa gcttcattct
     1261 ctcttgaaag cctctttcct ctaaagacgg aatctttata gttacaggta ttcttcttaa
     1321 aaatgtttcc aacattgctg tttctgggtc ttctgtagtg gctgcaatta tcataactct
     1381 acactcgtgt accttattag cctctcctaa ccttctatat atccctttat ccataagcag
     1441 aaatagcatt tcttgtccct ctgcagataa tctatgaact tcatctaaaa acaatactcc
     1501 attattagct tcatgtacaa taccatactt atctttatca gctccagtaa atgctccttt
     1561 tacatatcca aaaagctgcg ctaataataa ttgctgatta tcagaataat cagcacagtt
     1621 aaatatgata aatggtgcat tatcactatc ttcttttata gttaaactat acttatacat
     1681 atattctgca aatatagatt taccaactcc tgtaggtcct attattaatg tacttaaccc
     1741 cttaggtgga tatattactg ctgctttagc ctgttctatt tgggacctta aacttccatc
     1801 acatcctact aaattaaaaa tattgttctc tttttttata ctctctaaaa tatactcatc
     1861 tttgatagaa tcattttcac tatattcgtt tatagagtta atattatcaa atatcccttt
     1921 atttagtttc tttcctaaaa tactctcaat actttcttta tataagtact gaacaggctt
     1981 accctttatc tttataataa tactttcttt tattaattga tttaattcct tactagcatt
     2041 acttctattt atacctactt cagtagctat atattctgca tcaaatccaa atctaatgcc
     2101 attattaaag ttcaagtgtt cttctgttaa ccttttcaag caaattaaaa ctttttcttt
     2161 ccttgatagc ttcttttgtg tcatagttaa cctcccctat aagcagtaat actgttaaac
     2221 atttattatt aagcttaatt ctacaatatt ttaatatttt tgtctaatta tgtaactatt
     2281 gattttcttt cctattcgtt ttataatata aattacgtta ccctcatctt ttatagagga
     2341 ggaatttata tgaatttttt atcagattta cacacccact ctatagtcag tggtcatggc
     2401 tatagcactt tactagaaaa tattaattat tgtaaagaaa atggaattaa gatactaggt
     2461 acatctgaac atggtcctaa gatgcctggt tctccccaca gatggtactt tcacaacatt
     2521 caaaatatcc caagaatcat aaataacatt ataattttaa gaggctgtga agcaaatata
     2581 ctagatatta aaggaaacat agacctagag ccttttgtaa ttcctcgctt agattatctt
     2641 attttgtcat ttcacgaagc tgtgttttct ccaaatacat tagagaataa tactaaagct
     2701 ttaataaatg caataaataa gcatgataat attgaaatat taggacacct tggaaaccca
     2761 aactacccaa tcgactatga attaatcatt aaattagcta tagaaaagaa tatcttaata
     2821 gaaataaata attgttctat taaaggcgtg tcaagaaatg gtagctcgga taactgtaag
     2881 tatattgcta cactttgcaa aaaatatggt gctaaaatca tactgacttc agatgctcac
     2941 atttgttttg acataggtaa ctatgagtat tctgaaaata tcttaaagga aattaatttt
     3001 ccagatgaat taataatgaa ttatcctaaa aaacttatta atcattttca ccaaaaaggt
     3061 aaattactag atgttgatta tacaaatatc taagtaataa tacatgctta ctttaactcc
     3121 cataaaaatt ttaatactat ttatattata tgaaataaag aaattataat aaagaagcta
     3181 tttaataaag cttaattact tttatgaact tgatggattg atatggtcta tgatagcaat
     3241 agaaattata acatttttaa taaacttag
//
LOCUS       DAEMIK010000247         3234 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEMIK010000247 DAEMIK010000000
VERSION     DAEMIK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.252"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..684)
                     /locus_tag="KPK87_003707"
     CDS             complement(<1..684)
                     /locus_tag="KPK87_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2539809.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(684..1544)
                     /locus_tag="KPK87_003708"
     CDS             complement(684..1544)
                     /locus_tag="KPK87_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539810.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            1679..2479
                     /locus_tag="KPK87_003709"
     CDS             1679..2479
                     /locus_tag="KPK87_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG2539811.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTDLI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2504..3073
                     /gene="yfcE"
                     /locus_tag="KPK87_003710"
     CDS             2504..3073
                     /gene="yfcE"
                     /locus_tag="KPK87_003710"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG2539812.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDVLVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1217 a          469 c          385 g         1163 t
ORIGIN      
        1 ataattaaac ttattctttt ttgaaatctt aatagaaaaa tacattgata ttataaaaat
       61 tattattgtt aaaaatgtca tactatataa atttatatac ttagtattag ctatatcagt
      121 tacataattt gaaagatata ctcctccaac tttactataa ttaaaatgct ttacttgata
      181 tacaatcata ctaatcatct tcaaaagact tccatataca aagtaaatta aaatacttac
      241 tataaaacca caaactttca tttttccata tccaaactta aaaattaaag gcagtaaaat
      301 cgtcaaaatt gctaaaaata tatttacaga aaaaaataca tcatttaaaa ccatatttct
      361 aaagaacact agtgatgtca atttacttag taaggacaca attattgtta cagaaattat
      421 taatcctaca cctaataaat attttgagta aactatatct tctatactaa ctggcatact
      481 ccttataaaa atccttgaat cattttcata atcacactca aatgtattta taaatatcaa
      541 ataactaaca acaatactta ctgtataata tgaaattgaa ttaaggaaaa aataaaacac
      601 aaaaaatagc aatatgtact ttaaattacg gataccatct gaactaatcc ctattattaa
      661 atcttttctt attaaattga acatcatctc acctcattta cataataata cattagttct
      721 tctatagtag gatttttgac cttatgctta tcaatttcta tcaataggct ttttactttc
      781 aagctatctc caactttaac aagtgcctca ttataatatt tatactcttt cacaccaact
      841 atgctttctt taattgcttc taactctgct ttatttgccc taattatctt atattcttgt
      901 tctattaatt ctttcttaac agtaaaaatc tgatttcctc tatttataaa aacaagataa
      961 tcagcaattt taattaaatt atcagtatta tgagtagaaa ttattacact actacttcca
     1021 tttttcacaa aatcttgtaa gtactgcatt atttcagttc ttacaattgg gtctatacca
     1081 gcagttggtt catccaaaat caataactta gcattatgac taagtgcgct tgataacata
     1141 agcttctcag attgaccttt agataaattt tcaatatgca tattttcttc tatatcaaac
     1201 ttattaaggt attcatcaaa cctttctcta tcaaaattct tataaaataa agaaactatt
     1261 tttctaaatt ccttaacctt taaatgcgga taaaattgta aagaatcata tacaaaacca
     1321 atattgtttt taaagcttat tggagaattt tcaatagttt cattacaaag ttcaatttca
     1381 ccttcatctg catcaataat ccccattata atttttatta aagtcgtttt tcccgaacca
     1441 tttggtccta taatcccaac aatatcccct tcctttaact ctaagtttat attatttaac
     1501 ttgaattttc ctaatttttt tgtgatattc cttatgctaa acacatcttc acctcttatg
     1561 cattttttta tacccaccta tacatatttt aacacattca ctacaaaggt catttataaa
     1621 tttataaatt aagaattata aaactatgtt ataatacaaa gtatgaagag gtgaatatat
     1681 gaaaagaatt ataactataa atacaaacca attaaataag tcccttgaga ctaaagaact
     1741 cttaactaga aaacttatca atgctggctt tgaagtgtat tcagatattt atccagatac
     1801 agatcttatt atatcaattg gtggtgatgg ctctttttta agaacagtta gagatttcga
     1861 ttttcctgaa attcccataa tgggaataaa tacaggtcat ctggggtttt ttcctgatat
     1921 tttaccagat aagattgata gctttattga agcttataca aaaaaagatt atattattca
     1981 agaaatgtca cttcttaatg cggaagtata tacaactact agtggttcta atatgcttgc
     2041 tgtaaatgag gttgttataa gaggagacaa gtctaggact atacacctaa atttaagcct
     2101 tgataataaa catatacaaa attttagtgg agatggtatg attatttcta cttccacagg
     2161 ttcaacagcc tataactatt ctgctggagg tagtattgtt gatataaatc ttgaacttat
     2221 gcagattaca cctttacatc cgataaatac caatgcatat aggtgcttta catcaagtat
     2281 aatatgttca aatgaatctg ttataaagat tgcaccagaa tatagatttg aagattctgt
     2341 attgattgtc gttgatggag ttgagcacag atttagacaa attgaaaata taaaagttag
     2401 tatatctgat gcaaaaataa aattacttag aatgtcaaac tatgagtttt ggcatagagt
     2461 atctgaaaaa tttttataga ctgtacttag gaggattttt aatatgaaaa taggaataat
     2521 gagtgatact catggtagct tactttactt tgaaaaagcg ttaaatgtat tatctgattg
     2581 tgatgtttta ttacatggtg gagatgtctt atatcatgga cctagaaatg atattccaga
     2641 aggatataat cctaaaaaat ttattgagac attaaataaa ttagaaaaca ttgtgattgt
     2701 aaaagggaac tgtgatgcag atgttgacca gatggttata gaacatccta ttcagagtcc
     2761 ttatgtcatg agccaatttg gtgaaattag aattatattg aatcacggtt atatagaatc
     2821 agaagaagaa attattgaca aagcaaagaa aatgggtggt gacgtattag ttttaggaca
     2881 tactcatgta aaaaaactat acatggatga taatttaata gtaataaatc caggaagtac
     2941 ttctatccca aaagatggaa gccattctgt cgcaattata gatatcataa aaactgatga
     3001 tgaagatgag cttgaacttg atataaactt aattgacatc aatactggaa atataataaa
     3061 tatcaatgat tagtaaattt ttaataaaaa taaaataagc tttaatttat taaactcata
     3121 ataaaaacaa gtaaaccatt ttatttctat ctcaattaga agtttaagta caatacctta
     3181 aacttctaat taaatagaat ctggtttact tgttttatta tatgctatat tgaa
//
LOCUS       DAEMIK010000248         3141 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEMIK010000248 DAEMIK010000000
VERSION     DAEMIK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.253"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            309..989
                     /gene="pgmB"
                     /locus_tag="KPK87_003711"
     CDS             309..989
                     /gene="pgmB"
                     /locus_tag="KPK87_003711"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG2539813.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            986..>3141
                     /locus_tag="KPK87_003712"
     CDS             986..>3141
                     /locus_tag="KPK87_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG2539814.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINY"
BASE COUNT         1290 a          283 c          587 g          981 t
ORIGIN      
        1 gtaaaagatt tatatgaaga agaaatagtt gatttaaaag aagaactaat gttagagaaa
       61 tacaagttca ttattttaaa ataatacaaa aaagtattta ttttagattt catgttaaat
      121 tattatacag tttaaataag tctttataaa cgataaaaaa atatggtcta caagtgagtg
      181 aattgaaagt agattttaaa ttaaagactg agttagagta gataatttta taaataatat
      241 cttaaataca agatagaaaa taaattgatt attagtctaa aaatttcttg tagaaagagg
      301 aaataagaat gattgaagca tttatatttg atttagatgg tgtaattaca gatactgcat
      361 attatcacta tatggcttgg agaaaactag cccataaagt tgggatagat atagacacaa
      421 aatttaatga atctttaaaa ggcataagta gaatggagtc attggacagg atattagaat
      481 ttggaaataa gaaatattca ttttcagaag aagaaaaagt gagaatggct gaagaaaaaa
      541 ataattacta tgtatcattg atagatgaga taacttcaaa tgatattttg ccaggtatag
      601 aaagcttgtt aattgatgta aaatctaata atataaagat aggtctatct tctgcaagta
      661 agaatgcaat aaatgtatta aatcatttgg gtataagtga caaatttgat tttatagctg
      721 atgcaggaaa atgtaaaaac aataaaccac atccagagat atttcttatg tcagcgaaag
      781 ggcttaatgt aaatccacaa aattgtatag ggatagagga tgcaagtgca ggtatagacg
      841 ctataaactc agctaatatg ttttctgttg gtgttggaaa ttatgaaaac ttaaaaaaag
      901 caaatttagt tgtagattca acaaatcaat taaagtttga atatatacaa gaaaagtata
      961 atgagtatat agttaggaga attatatgaa atgtttattt agtaatgtag atgagtggaa
     1021 gattatacaa gataagattg aaatcaaaga aaatagatta gcagagtcca taatgagtat
     1081 aggcaatgga tatatgggta tgagaggaaa ctatgaggaa aattatagtg gagatacaca
     1141 caaggggtct tatatagctg gagtatggtt tccagataaa actcgtgttg gttggtggaa
     1201 aaatgggtat ccagaatact ttggtaaaat tcctaattca gtcaactata taggtgtaag
     1261 aattttcata gatgatgtag aattagattt agcaaaatgt aaagtagaag attttcatag
     1321 agaattagat atgaaaaatg gtattttaaa tcgtaggttt attgtaaata taaatgataa
     1381 aaaatttgaa gttagggttg ctaggttttt aagtatatct gttaaggaac ttgcagtaat
     1441 taaatacagt ataaaatcat taaattctaa tactaaaata aaatttgtac catatttaga
     1501 ttcaaatata aaaaatgaag attcaaacta tgaggaaaag ttttggaatg gaatttcttc
     1561 aagttcttta gaatataaag gtagtattct ttctcgaaca aaggagaatg catttaatac
     1621 accagtattc actgtgggtt caatgatgta tattaagaca aatggtgatg tagaagctgt
     1681 atctcatgag agtgattata attactgtga aaattcgata acaatggatg ttttaaaaaa
     1741 tgaggttgca agtatagaaa aatatgtatc tataacttca actagagatt ataaggaaag
     1801 tgagctatta agaaaatgtg aagacatttt aaatagagaa ctatctaaaa gttatgatga
     1861 tatattaaat gaacatacaa agttatggaa taaaagatgg gaaagtgcag atgtaaaaat
     1921 aaatggagat aaaagtagcc aacaaggtat aagatataat ttattccaac tattttcaac
     1981 atattatgga gaagattcgc gattaaatat aggtccaaaa ggatttactg gtgaaaagta
     2041 tggaggagct acttattggg atacagaagc attttgtgtt ccagtatatc taggtgtagc
     2101 agataaggag gtaactagaa atcttttaga atataggtac aatcagttag aacaggcaaa
     2161 ggataatgca aaaaaactag ggcttaaagg tgctttatat ccaatggtaa catttgatgg
     2221 agtagaatgt cataatgaat gggagataac atttgaggaa atccaccgaa atggaagtat
     2281 agtatatgca atatataact atacaaatta tacaggtgac tataaatata taaaagaaaa
     2341 aggaatagat gtaataatag aaatagctag attttgggca agtagagttc atttgtctac
     2401 aaaaaaagat ttgtatatga tacatggagt aacaggtcca aacgagtatg aaaacaatat
     2461 caataataat tggtatacta attatatagc taaatggtgt ttagattatg ctgtagaaaa
     2521 tatcttacga ttagagaaag agtgtaatga atgtattgac agaaataatg tgactaggga
     2581 agaagttgag ttttggaaaa taatatcaaa taaaatgtat ctgccgtatg atgaggaact
     2641 acaaataata attcaaaatg ataatttttt agataaagaa tttgtagaag taaaggattt
     2701 accaaaagaa aattttcctc ttaatcaaaa ttggtcttgg gataagattc ttaggtcgtg
     2761 ttttataaaa caagcagatg tattacaggg aatttactat tttggaaata gatttactaa
     2821 agaagaaaaa aagagaaatt ttgacttcta tgaaaaatat actgtacatg aatcttcttt
     2881 atcttctagt atttattcta ttattgcctc agaaatagat aacctagaaa aggcttatga
     2941 attatattca agaacagcta ggcttgattt agataattac aataatgata caaatgatgg
     3001 actgcacata acttcaatgt ctggttcatg gctttccatt gtacatggat ttgcaggcat
     3061 gagaacatgg aatgaaactc tatcttttga ccctaagttg ccaaaagagt ggaaaaatta
     3121 ttctttcaat ataaattata g
//
LOCUS       DAEMIK010000249         3107 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEMIK010000249 DAEMIK010000000
VERSION     DAEMIK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.254"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1077
                     /locus_tag="KPK87_003713"
     CDS             <1..1077
                     /locus_tag="KPK87_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG2539815.1"
                     /translation="DVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSI
                     CMGALSSGSTSVPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIM
                     SVLYEGGSIVISSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFT
                     GSQLLFEDTARNLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDI
                     YIREGKIFVNTEYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKI
                     ENEVKKIPQIEDAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKK
                     IIFLNSFPYTSSEKIDRLALLKML"
     gene            1153..2028
                     /gene="dpsA"
                     /locus_tag="KPK87_003714"
     CDS             1153..2028
                     /gene="dpsA"
                     /locus_tag="KPK87_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG2539816.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2031..2621
                     /locus_tag="KPK87_003715"
     CDS             2031..2621
                     /locus_tag="KPK87_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG2539817.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2828..3028)
                     /locus_tag="KPK87_003716"
     CDS             complement(2828..3028)
                     /locus_tag="KPK87_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539818.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1191 a          391 c          477 g         1048 t
ORIGIN      
        1 gacgttattg atttatatga tgataacaat aatgaagtag caaaaaaagt taatttttct
       61 atttttaatc ctaaatttaa tatatatatg tatcaatata agaagcttat aaattattta
      121 gataaaagta tttgtatggg agctttatct tcaggttcta caagtgttcc aaaggtatta
      181 tatagaacat atgagagttg ggctggattt tttcctatac aaaatggtat ttttaatatc
      241 tctggggaat caatcttgtt tgttaatgga acacttagtt ttacagggaa tctaaattct
      301 ataatgtcag tattatatga aggaggtagt attgtaataa gttcaggtct taattgtaaa
      361 tcatggataa ggacaataaa acaatataat gtaactaaca tataccttat acctacaaaa
      421 cttcaacttt tggttaaaca cttaaaagaa ccagtactca aagttgtaag tatttttaca
      481 gggtctcaat tattatttga agatactgca agaaatctaa aaaaatatat gccaaagtct
      541 gaaattattt tatactatgg ggcaagtgaa ttaaattata ttacatattt atgctatgat
      601 gaactaattg aaaaacctct aagtgtaggc agaccatttc caggaataga tatatatatt
      661 agagaaggaa aaatttttgt gaatacagaa tatgcagtat ataatgcaac caaaccttat
      721 tctgtcaatg atattggata ttatgataat gatggatact taatatttga aggaagaagt
      781 gatgacatta taaatattgg aggatttaaa gttagttcta ctaagattga aaatgaagtt
      841 aaaaaaattc ctcaaattga agatgcaatt gtattgccgt actcagacac tataagagga
      901 aatcaaattg tattatttgt aataactata gacaaaatta cgaagaaaga tttgttgatt
      961 aaaatgaaag ataatctaat gaaaaatgaa atcccaaaga aaattatctt tttaaattca
     1021 tttccatata cctcttctga aaagatagat agattagcat tattaaaaat gttataagta
     1081 taatatttag taattttagt gaaccaatgt attgataaaa acatattgta tatagtaaag
     1141 gaggcttaca atatgtcaaa tcaatacgat ataactgtta ttgggggaga cctgcgacaa
     1201 gtgtacatgg taaaaaaact aattaataaa gggtattctg tagtcactta tggtatatca
     1261 aatgaaatta taaatggaat cgttggaaaa tcaacttcac tagctgaagc actatccaaa
     1321 agttccatta ttttatgtcc aattccattt acaaagaatc aagttaatat tgaaaatacg
     1381 gatgattgtc ttgatgctac tattgataac ttgctaatta atttatctcc taatcaaata
     1441 ttatttggag gtaacatacc aactaaagtt tgtaaatttt gcgagcaaca ctctattcag
     1501 atttatgatt ttatgaaaat gaatgatgtt gctattttaa atgcagttgc tacagctgaa
     1561 ggggctatag cagaagctat taaaagaagt ataatcaata tacatcaaag taactgttta
     1621 gtattaggtt atggaagatg tgcacaaata cttgcaaaaa aattatacgc tttggatgca
     1681 aatgtatgtg ttggcgcaag aaataatgat gcaagaagta cggctaatgc atttggatat
     1741 tccagtattt ctttaagtca acttgatgaa aaacttttac aatttgatta tatttttaac
     1801 accataccag agtgtatttt aacgaaagaa aggttagaaa aagtatcaca agaagtaact
     1861 attatagata tagcttcttc tcctggagga gtagattatt ctgttgttaa agaagcaggg
     1921 attaatgcga ctctttgcct tgggcttcct ggtaaatatt caccaaagac atctggtgaa
     1981 attcttgtta acgctataga aaatattata aatgaaagaa gggattagaa atgagacttg
     2041 aaggacttac aattggagta ggatttactg gttcgttttg tacttatgat aaaatattta
     2101 tagagttaga gaatttggtt aaagaaggag ctaatgtaca cactatattt tcagatgtat
     2161 cacaaaatat cgattgtaga tttggaaatt ctgaagagtt tatgaaaaaa gcatatgaac
     2221 ttacaggaaa taaacctata gttacgattg aagaggcaga gccatttgga cctaaaggaa
     2281 ttgctgatat tataataata gctccttgta ctggaaatac tgctgcaaaa ttagcaaatg
     2341 gtattacaga ttcaccagta ttaatggcag caaaaggtca tcttcgtaat gataaaccac
     2401 tagtaatatc aatatcaacg aatgatgctc taagctttaa tttcaaaaat attggcattc
     2461 tccttaattc taaaaatata tattttgtac cttttggtca agataattgc aaagcaaagc
     2521 ctaattctat gattgcacat actgagctaa taattccaac tatagaactt gctctagaga
     2581 acaaacagct acagcctgtt ataaaaagtc cacatcaata gaattggttc ttataatatt
     2641 atgtattaga tacaatacta gcaaagaggc tgtctcaaaa tagagagaag cctctttttc
     2701 ttgttgaaat gatgaaaata tatttaattt catacaaaaa aatgcctcat ggactaaaaa
     2761 gtctattttg agctaatctc cgtaattttt gctatattaa aattatttac ttaatccaat
     2821 ttttaaatta gaatttatat cgttttgact cttcaaatat caatctaact gttgttgata
     2881 caaaagaaga ggacataatt aaaacaaata ccgatagtcc tctaataaac aaattatcta
     2941 cataataagt tactattcca atacctccaa tcgttaaaat tacacttata atcaaagaaa
     3001 aaacgctaat tgtttttaat ttttccatgc ttatcctcct tcttataaac ttcaaattta
     3061 tctatataat attaaatata ttttatttca tattatataa aaatcat
//
LOCUS       DAEMIK010000250         3015 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEMIK010000250 DAEMIK010000000
VERSION     DAEMIK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.255"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..1647
                     /gene="hflX"
                     /locus_tag="KPK87_003717"
     CDS             370..1647
                     /gene="hflX"
                     /locus_tag="KPK87_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG2539819.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1880..2377
                     /locus_tag="KPK87_003718"
     CDS             1880..2377
                     /locus_tag="KPK87_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2539820.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2427..2951
                     /locus_tag="KPK87_003719"
     CDS             2427..2951
                     /locus_tag="KPK87_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2539821.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIREL"
BASE COUNT         1254 a          267 c          530 g          964 t
ORIGIN      
        1 ataactactt aaatttaaga atagggttaa atataaaatg ataattacat ataagttaat
       61 taaactaatg tatttttaat atatatttag aattttaatc cttcttatga tataagtagc
      121 ttgttttgta ttacttagat ttgtaaggag tttttttatt gaaaaaactg cataatatat
      181 ttgggaggag gtgtaagaaa gtgatttttt atataagatt gagaaaataa tgcacgatta
      241 tggttattat aatagtaaat aagaagtgtc atttaaggat aagataaaaa gcaaaattct
      301 tgatgaattt aattatagag ccataatctc gtgctataga ttaaataaaa aatagcagat
      361 aaggagaata tggatatggc gaaaaaagga ataacagtag gattaaacat aaataataaa
      421 tctgaggact ttaatgaact aatgattgaa cttgaaaact tatgttcagc atgtgatatt
      481 gatgttgtag ggagtataac tcaaaatgca aagcaggtta atagagcatt ttacattggg
      541 acaggtaaag ttgaggaaat attaaactta ataaagaaag aaaatataga gatagttata
      601 ttttacaatg aactttctac atcacaacta aaaaatcttg aagaaaagct taattgtgaa
      661 attatagata gaacggcatt gatacttgat atatttgcac aaagggcaaa aactagagaa
      721 gctaaacttc aagtagaagt agcaagttta aaatatatgt taccaagact tataggttca
      781 aatgaaaatt taggacgtca aagtggtgga gttggtacta aaaatagagg ttctggtgag
      841 aaaaaattag aacttgaccg tagaagaata gaagaaaaga taacatcttt aaatagggag
      901 ttggatgatt taaaattcca aagagaaact caaagaagta tgagaagaaa atcaaatctt
      961 ccaaatgtag ctctagtcgg gtatacaaat gctggtaaat cttcaataat gaataaacta
     1021 gtagatatat ttaaaaactc tgaagaaaag aaagtgtttg agaaaaacat gttatttgct
     1081 acacttgaaa cttctgtcag aaatatagtt cttgcaaata acaaagaatt tttattatct
     1141 gatactgttg gttttgtaag taatctacca catgacttag taaaagcatt taggtcaaca
     1201 ttagaagaag catgtgaggc agacgtactg ttacatgtta ttgatatttc aaatccaagc
     1261 tataaaagtc atattaaagt gacagaagat actttaaaac aaataggtgc agatggtata
     1321 cctatgatac atgtgtataa taaaattgat ctaatagatg tagaagtatt ggatagaatt
     1381 ttagatagta tcgataaaga aggtatattt gtttcagtta aaaaagatat aaatatagat
     1441 aaaatgataa aatgtatttg tgatagcata tttaaagatt atgtaagatg taagttctta
     1501 attccatatg ataaaggtca tgtagtttct tattttaacg aaaatacgag cattataaat
     1561 acagaataca gagaagatgg agctatttta gatgtagaat gtagcaatat agaatataat
     1621 aagtataaaa agtatgcttt agagtaaatt ttgaaaaact tattatagcc taaaatatat
     1681 ttaatttata atttattata aattgattgt aaatatgatt ttatgaaacg gtatagccat
     1741 aatgctatgc cgttttgcca tttataataa aaataaattt attgacacaa tagtaactaa
     1801 ttactataat ataaagtaac tagttactat ttaaaattat caaaaaatta aaatctaaga
     1861 taaagagaag tgtataataa tggatgcaga aactttaatg aaaaaatata tggtagatga
     1921 tgatagtaga aaacaagcta tatttgcaag tatatttgta atgcaaaata aacttcaaac
     1981 tacatgtgat aaattagacc cagaattaac aatgaaacaa tggttacttt tagctattgc
     2041 ggactctgta gatgaaactc ttacattaac caaacttgga gaattaatgg ggtgttcaag
     2101 acaaaatgta aaaaaactag ctattgcttt agaaaagaaa ggttttatag aaattgagca
     2161 aaatgaaaaa gattcaagag ctatctgtct tgtgacaaaa gatagagtgc aagagtattc
     2221 taaaaaagta agaggtatgc aggaaaaagt attacagctt ttatttgaag attttacaga
     2281 gaaagaagtg gaacagttat tttttggaat tatgaaatta agtataggaa ttgaaaatgt
     2341 gaaaaattat gtagaaaata atgacgttgg agaataagca agtatcaaat caactttgta
     2401 tgacatcaga gaggggggga atttatatga aaaatgtagt agtgatttat aagagtaagt
     2461 atggttcaac aaaaaaatat gctaaatggc tttcagaatt attgtcttgt gatatttttg
     2521 aaactaagaa tattaatata gaaaaggtta tgaagtatga tacgattatt ttaggaggag
     2581 gtctttatgc ttgtaatata gcgggaattt ctttcttaaa aaaaaattat aaagaattgc
     2641 agaaaaaaaa tttagtagta tttggcgtag gagctgctcc atatgatgat gaagtaataa
     2701 agaaattaag agaagtaaat tttaaggatg aattaaacaa aattccttgc ttttattgtc
     2761 gtggggcatg ggatgagaca aaaatgtctt ttatagatta tactctttgt agttttttta
     2821 aaaaatctat agctaaaaaa gaacgtcaag gtcttaaacc agcagaagca cctttattgt
     2881 ctggagaagc atttgattgg tgtaataaag aaaatttaac tccaatactt aattatataa
     2941 gagaacttta agaggtatat atttataatt atttattatc taaagttaga tttcatagaa
     3001 acttactaga catta
//
LOCUS       DAEMIK010000251         2884 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEMIK010000251 DAEMIK010000000
VERSION     DAEMIK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.256"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1225
                     /locus_tag="KPK87_003720"
     CDS             <1..1225
                     /locus_tag="KPK87_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG2539822.1"
                     /translation="SPCILPILPIYLSILSNSSVENLKEGKTSFIGSSLFKNTIFFAL
                     GISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGLFYMGIIKSSILNREKRFN
                     VKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSSSNHLSANLLIAVYTIGFI
                     LPFIITAMFYSKLFKTIDKIRSNMEIIKKIGGIILIVSGILMMVNGFGSISKHFNTSQ
                     NSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKGSNDEDRIKSIDFTLTDQY
                     GKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDYNKNNDDVVILGVASPNLG
                     REGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPTTFIIDKEGYVTQYIPGAM
                     DKATMASFIENQRNK"
     gene            1346..2734
                     /locus_tag="KPK87_003721"
     CDS             1346..2734
                     /locus_tag="KPK87_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2539823.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT         1180 a          299 c          505 g          900 t
ORIGIN      
        1 ttcaccatgt atactgccaa ttttgcctat ttatttgagt atattgtcaa atagtagtgt
       61 agaaaattta aaagaaggaa agacgagttt tataggaagt tctttattta aaaatactat
      121 attttttgct cttggaatct caacaacatt ctttatattg ggttcatcag taaaagtatt
      181 gagtatgttt tttaatgaaa ataaagattt aataatgttt attggtggga ttataattat
      241 tatcatggga ttattttaca tgggaataat taaatcttca atattgaata gagaaaaaag
      301 atttaatgtt aagtttaaag aaatgaaagc aataacagct tttatattag gttttacgtt
      361 tagttttgga tggacacctt gtattggtcc aatattggca tctgtattag tcatggtttc
      421 aagttcaagt aatcatctta gtgccaatct tttaatagca gtatatacta ttggttttat
      481 tttgccattc ataattacag ctatgtttta cagtaaacta tttaaaacta tagataagat
      541 aagatctaac atggaaatta ttaaaaaaat aggtgggatt atacttatag tatcaggaat
      601 attaatgatg gtaaatggat ttggaagtat aagcaaacac tttaatacgt cacaaaatag
      661 taaaattgaa agcaagcaag aagaaaataa gcgagaaaat tctactgata aagaagaaaa
      721 ttctgatggt aatgatagtc aaaaagactc taataatgac aataatgata aagggtctaa
      781 tgatgaagat agaataaagt ctattgattt tactttaaca gaccagtatg gtaaaacaca
      841 taaattaagt gattatgaag gcaaagttgt attcttaaat ttctgggcta catggtgtcc
      901 tccatgcaaa gaagaaatgc cttatataga acaactgtat aaagactata ataaaaataa
      961 tgatgatgta gtaattttag gagtagcttc acctaattta ggtagagaag gttcaaggga
     1021 acatgtagtt aacttcttaa aagaccaagg ttatacattc cctgttgtac ttgatgaaga
     1081 tggagcatta gcatatcaat atggaattaa tgcttttcca actacattta taattgacaa
     1141 ggaaggatat gtaactcagt atattccagg agctatggat aaggcaacta tggcatcatt
     1201 tattgaaaat cagagaaata aataaaagta tttaagtata agtttataaa tcaatttaag
     1261 gttacaaaaa agtgcctaat tgtaccacct aaataatctt gttatagtaa aagtataaac
     1321 aatacaagaa agggttgaat tttatatgaa aaaaacattt gatgctataa taataggatt
     1381 tggaaaaggt ggaaaaacat tagcagggga tttggcaaac aggggattaa aagtagcttt
     1441 aatagaaaag tcaaacaaga tgtatggtgg aacatgtgta aatgtggcat gtataccaac
     1501 aaaaagctta gaaaattcag caaacagcgt aaaaacaaag aatataaatt cttgggatga
     1561 agtacaagct gaatatgaga aagctataga taaaaaagaa actttaatta caaaactcag
     1621 agaagctaac tataataaat taaattcaaa tgagaatgta actatattca caggaatggg
     1681 aactttcatt gatgaaaaaa cagttcaagt caaaactgaa aatgaaattt atgagttagt
     1741 agcagataat atatttatta atactggttc aagaccattt atacctaata ttaagggaat
     1801 tgaaaataag aatatagtat atgatagtga gtctcttatg aatctaagaa ctttacctaa
     1861 aaaaatgaca attataggag ctggatttat aggtttagaa tttgctggta tttatagttc
     1921 atttggagca gaagttacaa tattaaactc taataatggc attttaccaa atgaagatgt
     1981 tgaggattca gaagaaataa taaaattact tgcaaagaga aatgtaaaaa ttgttaataa
     2041 tgcaaatata aaagagatta aagaagtttc tgaattagca atagtagaat atgaagtaga
     2101 tggtaaatct aaagaattaa caagtaatat gatacttgta gctacaggaa gaaaagcaaa
     2161 cactgaaggg ctagggctag aaaatgcagg tatagaatta aatgaaagag gatttattaa
     2221 agtctcagaa actcttaaga ctaataaaga gcatatatgg gctataggag atataaatgg
     2281 aggtccacaa tttacttata tttcattgga tgattatcgc atagttatta atcagttatt
     2341 tggagataag actagaacaa ctaatgatag aaagaatatc ccaaattcta tatttataag
     2401 tccagcattt tcaagagttg gtcttaatgt taagcaagct aaagaaaagg gatatgaagt
     2461 attagtggca aagatgccag tagaagcaat tccaagagct aaacagattg gcaaggcaga
     2521 tgggtttata aaaatagtga tagataaaaa atccaataag atacttggag ctagtatgat
     2581 atgtgaaaat tcaagtgaga ttatccatct tattcaatta gctgtagatt tagaagtaga
     2641 gtatacatat ttaagagacc gtgtttatgc acatccaact atgacagaag ctcttaatga
     2701 tattctatct cctaatatga taaaagaagt ataacataag tttaatatat aaacctaaaa
     2761 ttatcattat aagtagattt tagaaagagg ctatctcaaa aaatagagat aagtctcttt
     2821 tttactgttt aaatgataaa aatatatttg attttataaa agaagattat cttatgaact
     2881 aaaa
//
LOCUS       DAEMIK010000252         2865 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEMIK010000252 DAEMIK010000000
VERSION     DAEMIK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.257"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            334..2144
                     /locus_tag="KPK87_003722"
                     /pseudo
     CDS             334..2144
                     /locus_tag="KPK87_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
     gene            complement(2232..2813)
                     /locus_tag="KPK87_003723"
     CDS             complement(2232..2813)
                     /locus_tag="KPK87_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539824.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1075 a          301 c          402 g         1087 t
ORIGIN      
        1 atagattatg tatatgccaa atttaaaaag agacataaag acattataca aatatattat
       61 tggggtgata ttttgtaaga aaagaagtga tgcagtaaat taattgagta atttaaaaat
      121 gatttaatga taatgttgat gaatgtttaa aaagaaattt tagaaaaacg tatattgctg
      181 tttgtgttgg aggtgatgaa tttgtagaat ttatagggta taaatgatta aactatttaa
      241 gataaataca tatcactgta aaatattgat aatcattttt tataagagat tgattatttg
      301 caaattatat atattacaaa tgaggtaaca ataatgaaga aaaaatatga ttttttaatt
      361 tattctgcta tttggctagt tattattatt ctttttatat tttctctata tcacagtata
      421 gagcatatta aaattataag tcacactggt actattcgta gtgaaactca aaaggttgtt
      481 aagcaagaat tgaataatga aagaaatgat gacttgataa aacgactgga taatattctg
      541 ataaagctaa gaacaggtaa tggagaaaat ggatttcaaa gatgtgataa taaagaattt
      601 caacaaaaac taaatcaaat ggattctatg tgggaatcta tgaagaaaga aattataaaa
      661 gttagaaatg gtgcttcagg tgataaatta tataaattga gtgaagagta ttctgtgttg
      721 tctaatcaaa ttgtttttat atctgaaaaa cactctaatg caaaattgta ttcttttgct
      781 actgcactat ttatttatct tatattctca acaataagtc ttttaatttg ggaatattat
      841 aataaaaaaa ggtttaaaag aatattttat actgataatt taacaaaaat aaaaaatcag
      901 gttgcatttg aaaacatagc gattgaaatt ttgcacaatg catctaacaa agagtatgta
      961 ctgctaaata ttgatattga caattttaaa tatattaatg atacacatgg atatgaatat
     1021 ggagataaag ttctaataat agttgcagct gcactttcta aaacttttaa tatcaaagaa
     1081 acttgtgcaa gaattggttc agataacttt gtaatacttg caaaatatag agattctctt
     1141 ttagaggata ttcgggaaat gctaactaat gctataatct ctgaattaga tatgaatgta
     1201 actcaaacta tatcttattg tataggagcg tacttagttg aaattgataa tttaggttat
     1261 aaatccatca attctataat ggataaggca aatattgcac acaaggtaag taaaacaaga
     1321 ggaatttcct ccacagtatg gtataatgaa aatctgttaa aacagttaca aatggaaaat
     1381 agtatttata attatatgta taaagcgata gaaaatgaag aatttcatat gtatttgcaa
     1441 cctaaatttc aaatatcttc tttaaatgtt gtaagcgcag aagctcttgt aagatggttt
     1501 tctccagaac ttggtttttt atcaccagat gaatttattc cattgtttga aaaaagtgga
     1561 tttattattg aattggactt ttatatgtta aaaaaagcgt gttcatttgt aaaaaaaacg
     1621 tttatgaaaa aaatcaatat acatatccga ttgcagttaa tttttcaagg gttactattt
     1681 accaaaatag tttttatcaa aggtttttag atactgttaa agaatatgaa attcctttta
     1741 agtatataga gatagaggta actgaaagtg cttttaatga aatatctcaa ccagttattt
     1801 caattttaga ggaacttaaa aagttaggat ttttaatttc aatggatgat tttggttcag
     1861 gatactcttc attaagtctt ttatgttctc tatctattaa tggattgaag ttagataaaa
     1921 gcttacttaa agaaaccttt aaccgggaga aagtatatag tattattcaa tgtattattg
     1981 aaatgtcaca tcgtataggc atgtctgttg tatgtgaagg tattgaaact aaaaaagact
     2041 tggaattttt aaatacagtt aaatgtgatg taggacaagg gttttatttt tctaaaccga
     2101 tagaagaaaa agaatttttt aacaaatatg taactaaaaa ataatataat tatttttatc
     2161 agtctataca ttgagtcatt ttatatctcg ttaaatgact caatgtattg ttatgtattt
     2221 taaaatataa attatttaaa acgttttttg tacttatata gaaattcttc tatatttttt
     2281 gattgttctc ttatttcaac tgcttctata tctagtaaat aaagtataga atcatttgta
     2341 agttcaacat attcaccatc ttctgaaccg agtgcatgtc tctcaatttt taatcctttt
     2401 tcttcataat atttttttgt ttctatatat agtgattctc gcttttgtat gtagtctttc
     2461 acttcattat ttggaaagtt atatgaatcc actaatgttt cgccatttat aaaagttttc
     2521 tcggtagcaa ctttttcatc ccaacaatgg atttcttccc aagtaaaaaa tatctcagtt
     2581 ccatctggaa tgaatgaaaa cctggtaata atatataaat aattattgaa atttacttca
     2641 taatattcag gaatatcttc aaattccata tagtaatttg tataattaaa attactatta
     2701 tcttttttta caaagggtat tttttcaata aatttatata ttgattgttc atttttaaaa
     2761 attcctatat cataactttt ttcaaattga ttaattttta ataaatacaa cattctttct
     2821 ccttttattc aaaatataat ttttataaat ttactaatct ttagc
//
LOCUS       DAEMIK010000253         2845 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEMIK010000253 DAEMIK010000000
VERSION     DAEMIK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.258"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            277..1038
                     /locus_tag="KPK87_003724"
     CDS             277..1038
                     /locus_tag="KPK87_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539825.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            1041..1859
                     /locus_tag="KPK87_003725"
     CDS             1041..1859
                     /locus_tag="KPK87_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG2539826.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            2039..2641
                     /locus_tag="KPK87_003726"
     CDS             2039..2641
                     /locus_tag="KPK87_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG2539827.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         1208 a          238 c          475 g          924 t
ORIGIN      
        1 taactgtaca aaatatattg attgagttta gattagcaat aaaaagatat atttattaaa
       61 tttgtgctaa ctgtacggaa tatactgctt aagtttagat tagtgataaa aagatatatt
      121 tattaaattt gaaccaactg tacaaagcta tactaattaa gcttagatta gctatataaa
      181 attataacaa taagtgtgtg ggcagaaaaa ttctgctaca cactttattt atattcacat
      241 aaaaaattta tcaaaaaaaa gaaaaaggag taaaaaatgg aatttacaag agaaccattt
      301 atactagatg acgatttgtg tctagctttg gtggacaaaa gaattagtta tctaatggaa
      361 aatgaattaa aattaagaaa tatagagata ataaaaacaa tagaatgtaa agaattatat
      421 gaaagcataa aatatcaccc ggatatatgt atttgtaatt taggaaaagg agatataatt
      481 gttgcgccga atgtatataa tcaatacaaa gagttattaa gtagatataa atttaatata
      541 attaagggag aagctaattt attaaataga tacccttatg atatacctta caatattgcg
      601 atagttggtg actatgctat acacaatttt aagtatactg acaaagcaat attagattat
      661 attgaaaata ataaacttaa aaagataaat atagagcagg gctattctaa atgctcaatc
      721 tgtatagtag atagaacgtc tataataaca tcagataaag gtatttggaa aagtatgaag
      781 aatacagaaa tagagtgttt attgatagaa aaaggtcaca taaatttatt tgagatgaat
      841 tatggattta taggtggatg tacagggttg atttctaagg ataaactagc tttttgtgga
      901 gatgttaaaa aacatcctga ctatgaacgt ataaaaagtt ttgtagaaag taaaaacaag
      961 gagatagtta cacttagctg tgaaaattta ctagatatag gttctatagt tcctctcatg
     1021 acaagaaagg agcgataact ttggttgaga aggataatat ttcttccatg aaatttttga
     1081 ggaataataa tgtaaacgat agattaatat atacgaatga cattgtagaa gtagtgatag
     1141 attctgcaaa gcaagaagtg ttaaataaaa aaatacttaa tggtactaaa gctgaacaaa
     1201 gagctttaaa tattgatgag atatcaacgg aaataactag tatgataata agtataatga
     1261 aagagaaact tttgaaagaa tacattctaa aatgctatgg gcgtacatat cctgaagata
     1321 aaaataatat atacatatat tcactgaaca tatttgctaa aaaagagatt tttatgagag
     1381 aaacagtatt tactagggtg agaaattata tagaagaaaa tgattttata aatgtagaag
     1441 gttttattag atttagaatg agagaattta tgaaatatat ttctgctata ggtgatattg
     1501 cagttgagga gtatttaata aagaaagacc aagatgaatt tataagagtt ttaaaatact
     1561 ttatagatac tcaagaagaa aagattgatt tgcttaaagt tcatataatg gaagataata
     1621 catttgtttt atacgataaa aatggaaata agatagacag tatagatgat gaagaaatta
     1681 taaatatggt aataagagaa aatttaaatt atgaggactt tttaataagt accttattaa
     1741 ctctatgtcc caaaaaaata gaaatattgg atttgttaaa taataattgc tcaaaggaaa
     1801 ttatagatac tgtagaagca atttttgaaa ataaagttag tataattatg gaaaactaga
     1861 atctaagcat ataaaagcta tccatataaa attaaaaaaa ttctttttaa atcgacacaa
     1921 aagacttata attgtatata aagctatagt ataactagag ttattaagta taaagaatat
     1981 ctacttgatt aatgcaatat agctggcaaa tatacaattg ataaggagta attttataat
     2041 ggataattta attaggatac tgatattagc tgttattggt ggatttatag gttatgttac
     2101 gaatgtagtt gctataagac tgatatttag accaatagag cctattaaaa tacctatttt
     2161 aaatatagaa atagtaggtc ttataccaaa gagaagagca gaaatagcag caaatgtagg
     2221 agaaataatt caagaagaat ttctttctat ggacgaaata cttgcaaata tcattactga
     2281 cgaagataag gaagaagttg taagatacat aaaagcaagg gtaaaaatta taatacatga
     2341 gaaagtttca tttatcccga gtggtataaa aaacatgata caagattatc taggagagat
     2401 aattgagtct gaagttaagc aaagtataga tgaattgagt aaaaatataa taaataaagc
     2461 aaatgaacgt atagatatac aaaaaatggt cgaagataag attaatgaac tagatttata
     2521 tgaattggaa gagataataa taagaatagc taagaaagaa ttaaaacata tagagttttt
     2581 ggggcttgtc ttagggtttt taataggtat agctcaaggg ttaattacta tgtttattta
     2641 aatatatgag aagtagcatt tatttaagta ctaattatat ttacctattt gagtgaatta
     2701 cttattaata acatttagat attaattgta tttacctatt tgagtgaatt acttattaat
     2761 agcatttaga tattaattgt atttacctat ttgagtgaat tacttattaa tagtatttag
     2821 atattaattg tatttaccta tttga
//
LOCUS       DAEMIK010000254         2824 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEMIK010000254 DAEMIK010000000
VERSION     DAEMIK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.259"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..292
                     /locus_tag="KPK87_003727"
     CDS             104..292
                     /locus_tag="KPK87_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539828.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            552..1751
                     /locus_tag="KPK87_003728"
     CDS             552..1751
                     /locus_tag="KPK87_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG2539829.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1803..2306
                     /locus_tag="KPK87_003729"
     CDS             1803..2306
                     /locus_tag="KPK87_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539830.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            2413..2730
                     /locus_tag="KPK87_003730"
     CDS             2413..2730
                     /locus_tag="KPK87_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539831.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
BASE COUNT         1162 a          192 c          529 g          941 t
ORIGIN      
        1 agtgctaaaa gatgtgtata aataataatg atgtcagttt ataatgatat taaattgaga
       61 tatactgttt ctctaaatta aaaaataaag gagtaaatgt gttatgaatt atcatacaaa
      121 agaagaattg gtggaagcac tgagagtagt atcttcaagt attattaatt gtggaaaagg
      181 acagaagaaa ttttctgagg agacatctca tcatacacgt tttaagaata ttatagaagt
      241 aatgtatatt tcaaaatcat taataatgga tgaaattagt aaaagagatt gaagcttatt
      301 ttgcaattta acatgtttta atatacattt tattatataa gaaaataagt aaacatacat
      361 gttataaaat attggaataa tatacaatta taaatttgtt aacactaata tctatatgtt
      421 aaaatatagt agaagatgtt agtaaataat aggatgagtt ctattttaat acaattaaat
      481 taagcttagt ttgaaatttg tatctaaaaa gtaaagtaat ctagaattaa gaaaatggag
      541 atagaattat aatggaaaag ttatattata caaatcagta tataagagat tttacagcag
      601 aaataattga aataaaagaa gtagaaaata agtatcatgt tttattagat aagacagctt
      661 tttttcctgg tggaggaggt cagtttggag acctgggaaa aatagagaat gatgatgtaa
      721 taaatgtgta tgaagatgga aaaaaaatat accatgtatt agataaaaaa cctataaaga
      781 tacataaggt aaagtgtagt attgattggg atagaagaga agatggaatg catcaacatt
      841 ttggacaaca tgtattgtct ggatgttttt ttaaaacttt taatgcaaat acgacaggtt
      901 ttcatttggg aaatgagtat agtacagtag atatagaagg taatttagaa gaggataaaa
      961 ttagagagat agagttgttt gccaatgaaa ttattcgtca aaatatacaa gtagagacat
     1021 tacttccaac gaaaaaagaa ctaaaaaaga tttggttaag acgtgatttg ccaaatactg
     1081 atgaagagat aagagtaata aaaataggtg atttagatac gaatgcatgt tgtggagtgc
     1141 atccaaaatc aactggtgaa ttgagaatga taaaaataaa gaaatgggaa aaacataagt
     1201 caagtacaag aatagagttt ctagcaggta aaagggcagt ggatgaagta ttgaagaggg
     1261 atatatattt gactaaaata tgtagatatt taagctgtag tgaggaagat gctataaatg
     1321 gtattgtaaa tctaaatagt aaagtggaag aggctttaat taaaaaaaga aggcttgaag
     1381 aagtagtatc tacatatcag gtcaaagaaa tgattgaaaa agcaaataag gttggcaata
     1441 tttcggtcat taaaaaaatt tatgatgatg aagatttgaa atatgtaaat aagatagcaa
     1501 ataaaattgc agaatatgaa aatagtattg cattattagc tgttaaattt aatgataaaa
     1561 taaatttagt ttttgcatgt tctaaaaatt taaaagatat taatatggga gttttattaa
     1621 aggatgcaat aagtcttata gatggcaagg gtggtggaag taaagtatta gctcaaggag
     1681 gaggtaagaa taatggaaat ttagaggcta cacttgatta tgcatttatg aagatagaaa
     1741 aaactctata aatatattat ataaaaaacc ttagtagata aaatagatat ggaggaataa
     1801 gtatggagca acaaaaggac tacatagagt tatatgaaaa tgaagaacta atagagataa
     1861 gagaagcacg tatggatgat ttggatacaa tagcaaaatt taattataat ttggctaaag
     1921 aaactgaagg aaaagaactt gatatggatg ttttaactaa aggtgtaaaa gcattgttat
     1981 tagatgagag aaaaggaaaa tatcatgtat atacagtatt tgataaagtt gttgcacaga
     2041 ttatgtatac atatgagtgg agtgattgga gaaatggaaa tttcctttgg attcagagtg
     2101 tatatgtaga taaggaatat agaagaaaag gtatctttaa ttatttattt aattatataa
     2161 aaaatatatg tgataaagat gaaaatatag ttggaatgag attatatgta gaaaaagaaa
     2221 acataaatgc aaaagcaaca tatgagtctt taaatatgta tgagtgtgat tataatatgt
     2281 atgaatacga agtagtacat tcgtaatata aatttgtaat actgttatac tgatgttata
     2341 aagtattgat tatattgacg attatatgaa ataaactgga tattataatt agaattttta
     2401 gggggtataa aaatgggcgt tttaaaagga atgttttatg tatttttatt tttagattta
     2461 gtgtcaatat tctgtttctt tttcaataaa ggaaaaatag catctaataa aattgtgttt
     2521 aatgcaatag gagtacttac atttgtactt tgttttatgc tttttagtta ttatcctaat
     2581 aataatctta ttggaaaatt tattgcttct ctatttttta tatttggagt tgcaggagtt
     2641 atgttgaaag aaaaaaattt tttatatgct agacttcttt taactgtagt tatagttttt
     2701 tctacattaa gattgttcgt tattcaataa aatgtatttg tatgaaaatt tattaaagtc
     2761 tacacattta agtgtaggct tttttgattt tttgtttgaa aattaaggaa tttttaagga
     2821 aatt
//
LOCUS       DAEMIK010000255         2785 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEMIK010000255 DAEMIK010000000
VERSION     DAEMIK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.260"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..544
                     /locus_tag="KPK87_003731"
     CDS             104..544
                     /locus_tag="KPK87_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2539832.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            560..2011
                     /locus_tag="KPK87_003732"
     CDS             560..2011
                     /locus_tag="KPK87_003732"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG2539833.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(2102..2770)
                     /locus_tag="KPK87_003733"
     CDS             complement(2102..2770)
                     /locus_tag="KPK87_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG2539834.1"
                     /translation="MFKIFLFSSEQFVNLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1149 a          263 c          488 g          885 t
ORIGIN      
        1 tatagtataa ttataaaaag aaaagaagta ttatattata gatatataaa cgcatttgag
       61 atataagagg gagttataaa tcattagttt tgaggtgaat gttatgagcg ataaagaatt
      121 atctattttg ttgttagaaa gtatgaataa ttttcataat ttaataaaga ttattaataa
      181 cgaaagatat aaaaaagata aggttcttac agaaagacag ttctttgcct tagtaaaaat
      241 aagaaaacat gataaaatag agcttaaaaa tctaagtaga gatttgcatg tatctacttc
      301 tagtctgtgt atactactta ataaattggt tgagcaagag tatgtatata gggaagaaga
      361 tagtagagat agacgaaata ctttttatgg aataactaaa aatggagaaa agatattgga
      421 taatgaaata ctaaagtttg tatctataat aagtgataaa atggattgcc tagatataga
      481 taataaagat aaattattta cttctttgga agagtctaaa aatataatag aacaattatt
      541 ttaaaaagga gaatttatta tgaatataaa gtattctaaa gaaatattaa aaagtttaga
      601 aaatgctata acagtagtag ctctatttga gggagaagat gtttctacga catttgataa
      661 aataaatatg ataagcagaa attttaattc taaagaaaat agtatagata ctgtaacatt
      721 gattgaaaat aacaatttgg taaatgtttg tcttgttggt tttggtaaga gagagttatt
      781 gaatagagaa aaaattagaa tcataggtgg taatttgtgt aaaaaattaa aaaatatatt
      841 gaaaaatgaa gcatataaga gtagtgatgt taatgtactg aatcttaatt taaatagtga
      901 tgaaataggt tcttttgtag aagggatatt acttggaaat tataagtttg ataagtataa
      961 gacaaaaatt aaggattttg aaagaaaaga agttgatact ataactatat ttactgaaaa
     1021 agaagttgag tgtcaaatag aaaaggctaa aatattagct aattcaacta taatagctag
     1081 aaatctagtt aatgaaccat caaacgtaat atatccaaaa acattagcag tagaaacagt
     1141 taaacttggt gcagagtttg ggtttaatgt tgatgtttat gaagaagaaa aaataaaggc
     1201 attgggaatg gatgcttttt tcgctgtttc aagaggctca attaataaac caagattcat
     1261 agtaatgaga tactttggag ataaagaaag tacagatata ttaggtttag taggtaaagg
     1321 attgacttat gatactggtg gatattcttt aaaatcaaat gcttctatgc tagatatgaa
     1381 gacagatatg gctggtgctg cgagtgttat aggtgctatg tgtgctatat ctcaaagtaa
     1441 attaaagaaa aatgttatag cagtagtggc tgcatgtgag aatgctttat caggaggttc
     1501 atacaaacct ggagatataa ttagttctat ggcaaagaaa actattgaag ttttaaatac
     1561 ggatgcagaa ggtagactta cattagctga tgcaatatac tacattataa acaatgaaaa
     1621 agtaacaaag gtggttgatg tagctacatt aactggtgct gctttaacat tgttaggtaa
     1681 tgttgcaact cctatagtaa caaataatga tgatttttat tgtgaattag aaaaagctgc
     1741 cactttatca ggggaaaggg tatggaagat gccaatttat gatgaattta aagatatgat
     1801 aaaaggagaa gaagcagacc ttaaaaacac tggtggtaaa aatgctggat gtataacagc
     1861 aggagctttt attggtgaat ttgttgggaa tactccttgg atacatatgg atatagcggg
     1921 gacaagtaca tcatcaaaat caattggata caaagcaaag ggggctacag gagaacctgt
     1981 tagaacattg tactatttgg cagaaatcta aaaaacaatt ataaaatata atttataaag
     2041 ccacgtggat atttttgtaa tttaagatta tctatgtggc tttatttttt atttaaataa
     2101 attatttact ctttttcttt ttttcaagta taaaagtatc tctattaaat aaaagagcag
     2161 gtatgtacat aatactagta acaaaaataa acgtaaaaat agcagttgtg ctatgactta
     2221 aaaaaccata atagtaccta gagttggtaa atgtgtttag taaaaatatg aatgaaaaat
     2281 aaataaaaca tatagctaag ttatctctaa gtgcatatct gttaactcta aattttctaa
     2341 catcaaccat atatatatct gcataaatta tcaagcactt agagaagata aagataaaat
     2401 atattaagct aggaaattca tagcccaagt ttttagaaaa aatgagttct ccaactgaac
     2461 aaactaaact ccaagaaaaa aatatattaa aaagatgata ctgcttaaaa attagtatgg
     2521 ctatacacag atatatacaa atataattga ttccaattgg taaagagttt agagtactat
     2581 aattacctga agaaataagt aataattgct ctagagccat aattattaca agtaatgtac
     2641 aaattatctt ctcaactgta tagctataag gtaaaatatt tttggttagt tttggacaat
     2701 agtataaaaa taatccaaat ataaagagat ttacaaattg ttcactagaa aaaagaaaaa
     2761 ttttaaacat ataacattct ctcct
//
LOCUS       DAEMIK010000256         2755 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEMIK010000256 DAEMIK010000000
VERSION     DAEMIK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.261"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(430..1137)
                     /locus_tag="KPK87_003734"
     CDS             complement(430..1137)
                     /locus_tag="KPK87_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2539835.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(1906..2691)
                     /locus_tag="KPK87_003735"
     CDS             complement(1906..2691)
                     /locus_tag="KPK87_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG2539836.1"
                     /translation="MNFENDFITSEFRKKYIGLVLDNAQNINKVYTTTFPDKEIIKQV
                     IDKDEKDILIFSHHAMGYIASDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDY
                     STSVSFAKAMGLEIVKPFCKYDDKIEVGVICKTSLKSIEEIKELIKKSVGHDVKLYDY
                     GDSVLKDGLVAIAAGGGSYPFVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDN
                     LINVIGATHYSTEKFACMGMVDYFKNLGLESEFLQGKYYLEDL"
BASE COUNT          959 a          440 c          243 g         1113 t
ORIGIN      
        1 tatagctctt taatcttttg ggtatatata cttaatcatt ttgaaatttg atttaaagta
       61 taataaaata tataaaaaaa tatataaaat ggtactataa aatttttaat cttcatcaat
      121 accttttttg ctttcattgt taaaactatt ttttctatta gtttatttct tataaaacaa
      181 acatttagta ttaaaatata tatcacacaa tagtatttgc aatttaactt taaaaaataa
      241 aaatacccaa ctgacctaag caactcgatt gggtataaat aatctaaaaa atcatttttg
      301 tataaactct ttatttaact tacattttta tttaccttct actttaattc aacttatatt
      361 tctatgcttc ttatactctc attcaactta tatttctact tatcttatac ttttataaat
      421 tcatcacaat tatttagttc tcgctatcaa agaaattgta ttaaaatact ttccattctc
      481 tacttccatc atttttttgt catttatagc atatgaatta tcacaattta tccaatcctt
      541 ccaagcttca tcatgacaat ttaaagcttc acatttctct acaatcacag actccgattc
      601 agaccaaagt ttattccacc atgtaattga atggaagttc atattatctt gccaaaatgg
      661 aatgagttca tctggtatac aatccacgaa atcttctttt aatccaggca ttgccatagc
      721 aagtattccc ccctctttta caagaggtga tatatgattg tctaaaaatc cttctttgtt
      781 tccaaaatag tgataagaat cgatacttat caccgcatca aagaaacctt ctgcataagg
      841 tagttcatgt gcctctgcct gtattggaaa tattttatca tccaatttaa actctttaaa
      901 tcttttatag ttttcagttg gttctatcca taaatctgtt gcaaatacag tagcatcaaa
      961 ttcctttgct aaaaatatcg aagatatacc tttaccacaa cctaagtcta aaatacgcat
     1021 tcccttttct agttttatct tacttgttaa ttcttccaat atttttatac aattaggtcc
     1081 catcatattt ttcatgagaa agtccttatc gtatttatta ctttttgtaa atatcattct
     1141 atctttcctt tccctatcag ttattattta ttaaatttta tacgtttgat tttttatttt
     1201 ttatatgctt cttatattga gcaatctctt ttttattcat tctttaatag gcaattttaa
     1261 tgtgtaattt tatccactct gtgatagtaa ctttaatgaa taatcttatc tagtgtgata
     1321 gttattttag tgagtaattt tatctactcc acagtaatcg ctttaatgag taatcttatc
     1381 cactctatga tagctatttt tatggacaat tttatctact ctataatata gtcactttaa
     1441 tgagtaatca taatttttat atcattttag atacttactc taataagaag tcattttcta
     1501 atacttaata agttttaatt acttccactt taacctatat actaattaac ttatttaatt
     1561 tagtgcatat ttgacacttt accttttatt ataccattta ttatcataaa tataataatt
     1621 ctattttttt aaaataaata tttataataa tctaaaataa atatttataa attctaatca
     1681 atcctaaata ataatctaaa atacatcaaa aaataaacta aaaaatatcg aaaaataatt
     1741 taaaagacat caaaactaaa tcttctaatc agcaattaaa agtctatttt cttagtttaa
     1801 aaaataaaga tagatttttt caaaacactg gataaatagg taaaaaaata aacagttagt
     1861 aaaataaatt tttactaact gtttattttt attgtttaat atgtcctata aatcctctaa
     1921 gtaatatttt ccttgcaaaa attctgattc taaacctaga tttttaaaat aatcgaccat
     1981 gcccatacac gcaaattttt ctgtagaata atgagtagca cctataacat taattaaatt
     2041 atcttttgct atttgatgga actctaatac tggttcaaaa tgttttaaag gcttagtaaa
     2101 cccagtaata tatagattta taccaagttc agcaacttct cttgctacaa atggataact
     2161 tcctccccct gctgcaatag caactaaacc atcttttaat acagaatctc cataatcata
     2221 caatttcaca tcatgaccaa cagatttttt aattaattcc ttaatctctt ctattgattt
     2281 tagtgatgtt ttacatataa ctcctacctc aattttatcg tcatatttac aaaatggttt
     2341 gactatttct aaacccatag cttttgcaaa actaacagaa gttgaatagt cactatagtt
     2401 gtctagtgga gaatgtaata cataaaaaga aattctacga tttttcatct cttccatata
     2461 agatagtggt atgtcgtgaa atggaaaacc ttcatctgat gctatgtatc ccattgcatg
     2521 atgtgaaaaa attaaaatat ctttttcatc tttatctata acctgcttaa ttatttcttt
     2581 atcaggaaaa gtagttgtgt atactttatt aatattttga gcattatcta atactaatcc
     2641 tatgtatttt tttctaaatt ctgaagtgat aaaatcattt tcaaaattca taaaactcca
     2701 atcatcatta atatttttaa tattaaattc taaatctaat tttttataaa gttca
//
LOCUS       DAEMIK010000257         2743 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEMIK010000257 DAEMIK010000000
VERSION     DAEMIK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.262"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            175..1098
                     /locus_tag="KPK87_003736"
     CDS             175..1098
                     /locus_tag="KPK87_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG2539837.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1073..1900
                     /gene="panB"
                     /locus_tag="KPK87_003737"
     CDS             1073..1900
                     /gene="panB"
                     /locus_tag="KPK87_003737"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG2539838.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDDCDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1919..>2743
                     /locus_tag="KPK87_003738"
     CDS             1919..>2743
                     /locus_tag="KPK87_003738"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG2539839.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDN"
BASE COUNT         1092 a          329 c          452 g          870 t
ORIGIN      
        1 attgtcaatt ttttatgaaa atggtaagat atttatggat ggtatcttgt tataagaact
       61 atactttaaa tgtttttaat tattttgtct tatgtaactc atatcctcag gaatgagatt
      121 agaaattaag atgttgtatc acaaagtgga ggtgatacta ttaaaatagg ctttattggt
      181 tctggaaaag ttggaacatc tttggcaaat tattttttat caaaaggtta ttgtgtttca
      241 ggcttttatg gaaggaatca attatcttta ttagaatcaa caaatttaac aaaaacaaag
      301 atttacagca atttgaaaga tataatttat gaaaacgata tattatttat tacaacatca
      361 gatgattcta ttgaaattat agacaaaaaa ctttccaaat ttaatttaaa acataaatac
      421 atttgccaca caagtggctc tttaaaatct agtatattgt tctgttcaaa aaaagcaggt
      481 gcattaattt attctatcca tcctatattt gcattttcca ataaaaatac caacataaaa
      541 aaaatgaaag acatttgttt ttccatagaa ggtgataatg aacaagatga tttagttatt
      601 caaaatttca tagatgattt gggaaaccaa ttttttataa gagataagga tacttcatcc
      661 acttatcatt tagctaatgt tttagtttca aatttagtat tatctttact agatatagga
      721 gtaagttact ttattaaatt aggtttatct gaagaggaat ctttaaaagc tatcagtcct
      781 ttagtaaaaa aaaatataga aaatatactt gataatggct ttactaaagc tcttactggt
      841 cctgtttctc gtggagatat aacaccaata agaaaacatc tatctgtatt ggataaaaaa
      901 gatgaggata tttacaaaat actatcctta aacctactaa aaataattgc cttaggaaat
      961 gacaattcat taaaaggtat aaaaaatatt actatagaaa atgcaatcga aaatttaata
     1021 agtaaatctg aaaaatacaa agaaatttac aaacttttag gaggaaaaaa cgatgaaaaa
     1081 taccatacag acttttaaga atgctaaata tgaaggaaaa aaactatcta tgcttactgc
     1141 ttatgactac tcaattgcaa aaataatgga tgattgtgat ataaatggta ttttaatagg
     1201 agattcatta ggaatggtta taaaaggtga agaaaacact ctatctgtta ccatagacga
     1261 aattatatat catacaaaag ctgtaaaaaa tggtgtaaaa aatgccttaa tcgtaagtga
     1321 tatgcctttt ctatcttacc atgtttctat agaagatgca gttaaaaacg ctggtagact
     1381 tataaaagaa ggaggagctc atgctgtaaa gttagaaggt ggctcaaacg ttataaagca
     1441 aatagaaagc attgtaaatg ctcaaatacc tgtaatgggg catttaggat taactcctca
     1501 atcagtaaat tcatttggtg gatttaaggt tcaaggaaat accagtgaaa cagcaagaca
     1561 acttattgaa gatgctaaat taatagaaaa agcaggtgca ttctctattg tccttgaagg
     1621 agtgcctacg aaaatagctg agatggtaac taattctatt tcaattccta caataggtat
     1681 aggagcagga attaactgtg atggacaaat tttagtttat caagatatgt tgggaatgtt
     1741 tggagatttt gttcctaagt ttgtaaagca gtatgcaaat ataggagata taatgaaaga
     1801 ttcaattaaa aattacatcc tagaagttaa tacaggtgca tttccacaag aaaaacatag
     1861 cttttcaata aatgaatctg agttagaaaa attatactaa tattaaggat ggtacaaaat
     1921 gcttgtaaaa gaaattaaat tactaagaaa tataatcaaa gattggagaa aacatggcta
     1981 ttctataggc cttgttacaa caatgggatt tttacatgaa ggacaccaaa gtcttataaa
     2041 aaaagctgta aaagaaaatg ataaagtagt agtgagtgta tttgtaaatc caactcaatt
     2101 tggaccaaat gaagatttta atagctatcc aagagatata gataaagatt ttaaatattg
     2161 tatggatagt ggagcaacag tagtctttaa cccatcacca gaagaaatgt atttaaaagg
     2221 taattgtact accattaatg tttctggact tacagatttt ctttgtggag ctaaaagacc
     2281 tgttcatttt ggaggtgtct gcctagttgt ttcaaagttt ctaaatatag taacacctga
     2341 taaagcttat tttggagaga aggatgccca acaattagct gtaattaaaa gaatggtcaa
     2401 ggatttaaac atagatacag aaataatagg ctgtcctata attcgtgaaa atgatggatt
     2461 agcaaaaagt tcgagaaata catatctctc tgaggaagaa cgaaaatctg ctctaatatt
     2521 aaataagagc ttaagtttag caaaagaaga attagtaaaa ggcaatctaa atccagagaa
     2581 tataaaagaa ttaataacag ctaaaataaa ttctgaacac ttagcaaaaa tagactatgt
     2641 tgaaattgta gatagtgaga cattacaacc tgttaagcaa atagaacatt ctatacttgt
     2701 ggcaattgct gtgtttatag gaaaaaccag actaattgat aat
//
LOCUS       DAEMIK010000258         2712 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEMIK010000258 DAEMIK010000000
VERSION     DAEMIK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.263"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            692..2599
                     /gene="ltrA"
                     /locus_tag="KPK87_003739"
     CDS             692..2599
                     /gene="ltrA"
                     /locus_tag="KPK87_003739"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2539840.1"
                     /translation="MPKKSKTLCVDDLRHAEYYGMQDTFDELYQKSQNGEVFENLMDL
                     ILSRDNILLAYRNIKANKGSYTAGTDKKNITDIGSRTPDDVVKRVRFIVTGSEHGYRP
                     KPVRRKDIPKPNGKTRPLGIPCIWDRLIQQCIKQIMEPICEAKFSNNSYGFRPNRSVE
                     HAINRTYTMLQMMNLHYVIEFDIKGFFDNVNHSKLIRQIWSLGIHDKTLIFIIKRILT
                     APIKMPDNTTVLPDKGTPQGGIISPLLANIVLNELDWWIASQWEENPIAISRGRERII
                     GKTKVFDKSHGYRIMKNTEMKEMHIIRYADDFRIFCRTKEDAVRTKEAVTEWIEERLK
                     LEVSPEKTRIVNTRKRWSEFLGFKIRVRLKHHKYVVQSAICDKKVEIERAKLVEQAKN
                     IAKPREKKSCLSEIQLYNSMVLGIQNYYQLATCISIDCREFHRRVMTVLTNRLNTETG
                     SMLKREGGTITQAEKERFGQSKMIRYVSGIDQMIYPIAFIKNKIPMAKRSIVCSYTKE
                     GRALIHTELNLNQYVLKGLREKISVGHSTEYHDSKISLFSAQKGKCAISGEEFADAEH
                     VAVWLKVPGSLGGFERYKNMVLIHKKYLILLQELPQAAIKDLIKTLNITKKMLVKINS
                     LREQANLSAII"
BASE COUNT          968 a          449 c          650 g          645 t
ORIGIN      
        1 gtaaaaagtc tgcgcacagt gttggtttat taaataataa ccagtctgtg aactggtaat
       61 ccgataggtg gaatgatggg gtaacgccct gaaacgtcta tccttgacgg acttgcgtca
      121 gctgtaatgg ctggggtgag aagctccgtg acaacgaccg agagtagtcg taggaatctg
      181 ccaaaagatt ctcgaaaacg ccccagaggt ggggtgcgct acctataggt ctaggatcta
      241 taaaatatct atggttagaa tgtttacttt ggcgagtaaa ctgacgaaaa ctgcgaatgt
      301 acggttctaa aagaagacca tatagaaatg tatgggtgcg ttaaagcgca gtcagtgtgg
      361 ttcagtagaa attgacccta cgaacgacca tgttgtgtta caggcacatc caagctgaca
      421 ggactatagc agagacctaa ggatatatgt acagatagga ttatcggaac gtggaaaggc
      481 atcagattcc attatgggat agcctgacga agaaaataag caggtgaact ggtgctgaaa
      541 agccgtgatc gaacttatga tgagttctgc gaaaaagagg aactcggagg aatggtcaca
      601 agtcggttga aataaacagg cattattcag tcttatgata aggattacga gtaagaccaa
      661 aagggtcact ttcgagagga ggtgatgcct tatgccaaag aaaagtaaaa cattatgtgt
      721 agatgacctg cgtcatgcgg agtactatgg aatgcaagat acttttgatg aactatatca
      781 aaaaagtcag aacggtgaag tatttgagaa tcttatggat ttgatactga gcagggataa
      841 cattttactt gcgtaccgaa acatcaaagc gaacaaaggc agctacacgg caggaacaga
      901 taaaaagaac atcacagata ttgggagtcg aactcctgat gatgtggtaa aaagagtgag
      961 atttattgtt acgggaagcg aacacggcta cagaccaaag cctgtaagac ggaaagatat
     1021 cccgaaacca aacggaaaaa cacgtccgct gggaatccca tgcatatggg ataggctgat
     1081 acagcaatgt atcaagcaga tcatggaacc aatctgcgaa gcaaagttca gcaataatag
     1141 ttatggattt cgtccgaaca gatccgttga acatgccatt aacagaacct acaccatgct
     1201 tcaaatgatg aaccttcatt atgttattga gtttgatatt aaaggatttt tcgataacgt
     1261 aaatcatagc aagttaatca gacagatatg gtcgctgggt atccatgata aaacgctgat
     1321 ttttattatc aagcgaatac taacagctcc aattaaaatg cccgataata caacggtatt
     1381 gcccgacaag ggtacaccac agggaggaat tatctcacca ctactggcaa acatcgttct
     1441 aaatgaattg gattggtgga tagccagtca atgggaggaa aatccaattg ctataagcag
     1501 agggcgagaa agaataatcg gaaaaactaa ggtttttgat aaaagccatg gttataggat
     1561 tatgaaaaac acagaaatga aagaaatgca tatcattcgg tatgcggacg attttaggat
     1621 tttctgtaga acgaaagagg acgcagtcag gacaaaagaa gcggttacgg agtggattga
     1681 agagaggttg aaattagagg tgtcccctga gaaaacaagg attgtgaaca ccagaaaacg
     1741 gtggtcagag tttcttggat tcaaaataag ggtaaggttg aagcatcata aatatgtggt
     1801 gcagtcggca atctgtgaca aaaaggttga aattgaaaga gcaaagctag tggaacaagc
     1861 gaaaaacatc gcaaaaccca gagagaaaaa aagttgttta tcagaaattc agctatataa
     1921 ctctatggtg ttaggcatac agaattacta tcagcttgcc acctgtatca gtattgattg
     1981 cagagaattc cataggcgag taatgacagt tttgacgaac aggttaaata cagaaacagg
     2041 aagtatgcta aaacgtgagg gtggaactat cacccaagca gaaaaggaaa gattcggaca
     2101 gtcaaaaatg attcgatatg tttcaggaat tgaccagatg atctatccga ttgcattcat
     2161 caaaaataaa ataccgatgg cgaagcgatc aatcgtttgt agttatacaa aagaaggtcg
     2221 tgctctgatt catacagaac ttaaccttaa tcagtatgtt ctgaaaggac tgagagaaaa
     2281 aatatccgtt ggtcatagca cagaatacca tgacagtaaa atatctttat tttctgctca
     2341 aaagggaaaa tgtgcaatca gtggagaaga attcgcagat gcggaacatg tagctgtatg
     2401 gctcaaagta ccaggatcac ttggtggatt tgaaagatat aaaaacatgg ttctgattca
     2461 caaaaaatat ctgattctgt tacaagaact gccccaagca gcaataaaag acctgataaa
     2521 aacactcaat atcacaaaaa agatgctcgt gaaaatcaat agtctgcgag agcaggcgaa
     2581 cctgtcagca ataatataaa acttaaattt ggtgactgat taaatgtctt gtgaagacaa
     2641 tcgatggagc gccggatgcg gtgaaagtcg catgtccggt gtgaagtggg ggaaaaggtg
     2701 gagataatat ca
//
LOCUS       DAEMIK010000259         2674 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEMIK010000259 DAEMIK010000000
VERSION     DAEMIK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.264"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..504)
                     /locus_tag="KPK87_003740"
     CDS             complement(<1..504)
                     /locus_tag="KPK87_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539841.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRY"
     gene            848..2278
                     /locus_tag="KPK87_003741"
     CDS             848..2278
                     /locus_tag="KPK87_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2539842.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSCVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         1145 a          270 c          415 g          844 t
ORIGIN      
        1 atatctgtca atagactcat aacctagatt tagaatgttt ttgtctttta tactatctac
       61 atctaaaata tatgatgggt cagcctttag taatatatta attaaacatt tttcttcacc
      121 agcacagaat aactccatct tacttgatat agatttagat tgctctgaag ttatttttgc
      181 aggaataagg ttgtcattat catctcttag aagataacca tgtgtgtctt gttgatatac
      241 taaattaacc tttttcttag gaacactaat cattttatct aaatgtacat aatgatgatt
      301 ttcaacaaaa ccttcattta atatttcaac aaagttatta attttaccta ggcaaaaaga
      361 ttctatagat tctttacaga atttatttgt aaatggtatt atgtttacgt tttcctcgtc
      421 tgtattccat gctatagcat aatccaacga atatacttct tcctctatat ctgcaaaatt
      481 atattgtact atgtttcctt tcaaaattac tcgacctcca tcttataata tatttaatat
      541 actttataaa acttatatta tatttaaata atatcataaa ttgaataaat atgatatagt
      601 aaataagaaa aaatttgaaa aaaatcacta taaatgagta ataaatataa atagtatata
      661 ctaatgggat ggaaaatgtc cactattaac tttattaccc cacatatata agataaaatt
      721 attaatataa aatataatct atcgacaatg aatagacttt taaataaact taatataaaa
      781 ttaattaaat aagtgcttaa aaacgattta ctttaatgat agaatcatat taaaggagga
      841 taattttatg gatataaaga ataaaattaa atctatattt ttaccagaaa aaaatgacta
      901 tttagatgat gaagaatttg agtatgatat agaaaataat gaagaagatt taaaggaaga
      961 taacaaagta catttaaatg atttaagcag agtaaaatat gattacagag agttaaaaga
     1021 catagaaaac caaacagaag agatgtgtct gctagcagta aagcaggatg gtactataat
     1081 tgagtttgta aaggacaaga cttataaagt atgtatggaa gcagtaaaac agacatataa
     1141 atctcttaag tatataacaa atcaaaatga ggatatttgt atagaagcag taaaacagaa
     1201 ctacagagct ttgtactaca taaataataa gacagaaaat gtactaattg aggctataaa
     1261 aaatgcatca acttatgatg tcatggaagt atttaaattt gtggaagagc aaacagaaga
     1321 tgtatgtcta gcatttatag aaagggcatc taaaaatgat gttgcagaga tattaaaagg
     1381 aataaaagag caaactccag ccatatgttt agaagctgta aaaaaagatg gaaaatcttt
     1441 agcttatgta aaggaacaaa gtaatagtat atgtttagaa gctgtaaaag aaaactacag
     1501 tgcactatct tgtgtaaaag agcaaactga agaaatatgt atagaagcag taaaacagaa
     1561 tgactttgca ttgtattatg taaatgaaca gacagaaaaa atatgtatgg aagcagtaaa
     1621 aagaagttat atggctctac agtatgtaaa taagcaaaca gaagagattt gtttggaagc
     1681 agtaagaatt gatggaagag cattacagta cgtaaaagag caaacagaag aaatttgctt
     1741 ggagtcagtt agacaaaatg gaaaagtact gcaatatgta aaaaaacaga ctgaaaatat
     1801 atgtatagaa gcagtaagag gttcatttga agaacttgaa ataaaagaaa tattaagttg
     1861 tgtaaagata ccaacagaaa gaatatttgt agaagcagta aagcaaaatg gaaaaattct
     1921 taaatatgta gaaaatcaga cagaattaat atgtttagag gcagtaagag aaaactataa
     1981 tgctttagct tatgtaaagg aacaaacaga aaagatttgt ttagaagcag ttaatcaaag
     2041 ttatgaagcg cttaaatatg tgaaagaaca aacagaagaa gtttgtctaa aagcagtaaa
     2101 acaagattat agaatgttga aatatgtaaa taatcaaaca gaaaaaatat gtttagaagc
     2161 agtaaagcaa aattatagag ctttagaatt tgttgataat caaacagaaa aagtttgtct
     2221 tgaagcagta aaacagaata gaaaagcact acagtatgta aaacaaaaac aaagttaaaa
     2281 ggctaaaata tatagaaaac atatgcaaat aaataaaatt tgatattttg taataaataa
     2341 aatgggtatc tcaaaaggaa ctttttagtt tgtgaggtac tctttttata tgaaatgaaa
     2401 tatattttta gccttttaac aatggggaaa aggtttattt ctattttgac atagcttttt
     2461 atgcatataa aggttgataa aattgtttta ttataattgt tttgttatat aaataataag
     2521 aagaaaatta tataggtctt tttttgacca gttttacatt tttaacaact agttttgtaa
     2581 agtatattga attttctcaa aaaagaatgt actctatata tgtaaaattt ttttggaggt
     2641 aaaaatgttt aaaagatatg ctgaaaaaat gaca
//
LOCUS       DAEMIK010000260         2628 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEMIK010000260 DAEMIK010000000
VERSION     DAEMIK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.265"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1065
                     /locus_tag="KPK87_003742"
     CDS             <1..1065
                     /locus_tag="KPK87_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG2539843.1"
                     /translation="IIKKKKDICDYLNKISNYKKVKSTIEKLETRGKMPKWYLELINK
                     VKNILENMLYNRNLSDEQIFYLNLFLDIQNLFLGIYELKSESVIFENTLDDNKIIAKM
                     DTYIIIDYINASFEYLGYVLNGSALNSIDYGVPQNRERFVLIGAKKDFLKNKKIETPK
                     PIVGENYVTVRDAIGDLAEYEASKGSMDYCFEKNSNNIEKDFYRKIILDSNKIYNHVC
                     TDTRNIALKRFEYINQGNNFHSLPDELKGTYADPERTQNTIYKRLVYDKPSDTVVNVR
                     KSMWIHPIKNRAVSAREAARLQSFPDSYKFLGTKDSVYQQIGNAVPPLLGRVVAEKIL
                     NLFNDEPNEYLRDVFEALDG"
     gene            complement(2065..>2628)
                     /locus_tag="KPK87_003743"
     CDS             complement(2065..>2628)
                     /locus_tag="KPK87_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG2539844.1"
                     /translation="LVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELRENTIN
                     NSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFMDYR
                     NNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFSVYT
                     ADLINRDYLSIHFNNNDEFKYSLNYIT"
BASE COUNT         1005 a          308 c          353 g          962 t
ORIGIN      
        1 attattaaga aaaaaaagga tatatgtgat tatttaaata aaatatctaa ttataagaaa
       61 gtaaaatcta ccatagaaaa attggaaaca aggggaaaaa tgccaaaatg gtatttagaa
      121 ttaataaata aagtaaaaaa tatattagaa aatatgttat ataatagaaa tttatcagat
      181 gaacaaatat tttatttgaa cttattcttg gatattcaaa atttattttt aggaatatat
      241 gagttaaaat ctgaatctgt aattttcgaa aatacacttg atgataataa aattatagct
      301 aaaatggata cttacattat aatagattat ataaatgcat cctttgagta tctaggatat
      361 gtattaaatg gaagtgcact aaattcaatt gattatggag tacctcaaaa tagagaaaga
      421 tttgtattga taggagcaaa aaaagacttc ttaaaaaata aaaagataga aactcctaag
      481 cctatagttg gagaaaatta tgttacagta agagatgcaa taggcgattt agctgaatat
      541 gaggcttcta aaggtagtat ggattattgt tttgaaaaaa atagtaataa tattgaaaaa
      601 gatttttata gaaagataat attggatagc aataaaatat acaatcatgt atgtacagat
      661 acaagaaata tcgcactaaa aagatttgaa tatataaatc aaggaaataa ttttcattct
      721 ttgccagatg agttaaaagg aacttatgct gatccagaaa gaactcaaaa tactatttat
      781 aaaagactag tatatgataa gccatcagat actgttgtaa atgtaagaaa atctatgtgg
      841 atacatccta ttaaaaacag agctgttagc gcaagagagg cggctagatt gcagtcattc
      901 cctgatagtt ataagttttt aggtacaaaa gattctgtat atcaacagat aggaaatgca
      961 gtaccacctt tattaggtcg agtagtagca gaaaagatat taaatttatt taatgatgaa
     1021 ccaaatgagt atcttagaga tgtgtttgaa gcactagatg gatagttcaa ataaaatagt
     1081 gggtattatt cttgtgattc ttatccaaca ttaatccacg aaataaggac tgtcgaatga
     1141 gtaattttaa attgctgtgc gacagttctt tttttacttg cttttcatta atttatattt
     1201 atgcaaaatt tgagcttgaa aacggtcata agaaataaag gtataaaaat acaattttaa
     1261 aatagaataa tttacaaagt gtaagattaa aaaatatttt tgcgaggatg aatatacata
     1321 ctacatttat taatgattat cttatttgtg aaacttagaa atactatagg ctaacactac
     1381 aggtataaac tctgaaagaa tattttgatt gcctttgatt agaattcttt taaagttcat
     1441 actatgtttt atttgggtga aagctcctta tgcctgaata cttctattca ttctaagttc
     1501 acatccttgt gtaccagtga ttctttctaa attatcttaa tgccaaaatc ttgttcgcat
     1561 ttcaaaataa agtcaggtag ctacttcaac attttagtta tatttcttca atagtttgtc
     1621 tccaaacaaa ggtacatcta ttagaaacag atttaatttt atttatatca ataaataata
     1681 tatactttga aacttcatta atatcaccaa gataatttat aagttaagct attatatact
     1741 atcttcacca actacatcct ccatagtttt acactttctt aattcagctt ggtaatctaa
     1801 atctgtaata attatttctc tttgattttt catttctttg cctacaaaag tttaatataa
     1861 atattattcc atacttacca attttacgtt caaaaaagac tgtagataag tctgcattta
     1921 cacaaaccta cttataggtc tcttttttga aattaattta gcatgaacta tagtaagagc
     1981 atctttaaat tcatagttac aagtatacaa agttataatt ttatctgaaa tatctacctt
     2041 tatatcagat ttgtgtactg atttttaggt tatataattc aaagaatatt taaactcatc
     2101 attattatta aagtgtatac ttaaataatc tctgttaatt aagtcagcag tatatactga
     2161 aaaaatttca taagtataag tttgtacttt ataatttatt tctactaagt tattttcttt
     2221 gaaaaatact tcatcagtat agttttctag ttgaccaaac atagttttat ttttcatata
     2281 atgaccatag actattaaac tcttatcatt ttcaaaatta tttctataat ccatgaaaat
     2341 actacctaaa ggaagataat ttttataaaa atcatgagtt aggtagaaat cattgttata
     2401 tccttgtact acaggatagt ttatatttac gttgtttatt ttaagccaga atctataatc
     2461 attattttta tcataaagtt cttgatgttt gctattattg atagtgtttt ctctgagttc
     2521 cgtatatact tcatcagctt ttttatattc agataatttt atgtaaatcg aaaggctaga
     2581 gaaagttagc gcaataatgc aaataatatt tattaatatt tttactag
//
LOCUS       DAEMIK010000261         2616 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEMIK010000261 DAEMIK010000000
VERSION     DAEMIK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.266"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(165..977)
                     /locus_tag="KPK87_003744"
     CDS             complement(165..977)
                     /locus_tag="KPK87_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2539845.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLDEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(1053..2471)
                     /locus_tag="KPK87_003745"
     CDS             complement(1053..2471)
                     /locus_tag="KPK87_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG2539846.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT          909 a          478 c          271 g          958 t
ORIGIN      
        1 ataataaaaa aataagaggc tgtctaaaaa tatagataag ccttttttca taatcgtaat
       61 tataaaaata tacttgattt catatgaaaa agagtgtctc ataaactaaa atgtttactt
      121 tgagacacca ccttttttat tcttatttat actttgtaga catattataa catgcgtttc
      181 actggtatct gaacttctgt caaccagtct tcttcactct ctttattcca tataccatct
      241 atataagatt ctctaggatt tccaaccact ttatatttat tgtcctctat ccatttaaat
      301 gcatctgcgt aagtttttcc taatgttgaa tatggaccat tgtgtaatat acaaactgca
      361 ttttcaactg catctaattc tttaaatttt aatatatccg tatcttcctt taaatcattt
      421 atagcttgac acatttcaac atccatatca gtctctctat attcaccatc atgatatata
      481 ttaaaacaat aatcaggaac tgcacatgta caccctgttt tcatcatttc ttcagccatt
      541 acatttggaa atagattaaa aagttcatca taccccttta acacttttct catagaagca
      601 actttaactt ttggtaaaga cttaataatt acatcattca tattgacttc tcctttcaaa
      661 ttcataaggt aattacgtag tttaattatt ttatattctt cacttttaat tacatccaac
      721 atttcttttt ctttattcat taaaatattt tcaacttctt caggattttc tacagctttt
      781 tttatctcat ctaatgataa acccatttgt tttaaagtta ttatctgatg taatttaggt
      841 aattgctctg ttgtataata cctatatttt gtaaattcat ctacatattc tggttttaac
      901 aaccctatat cttcataata ccttaatgtc tttgtagtta ttttatttat tttagaaaac
      961 attcctatag aaaacatctc atcacctgcg cattagactt gattaattta agtttaaact
     1021 ttccccttag gttaaagtca atacaaaata aattataatt gtattaattc ttcaattcta
     1081 tctccactca ataccaaaat atcccctgtt tttataatgg ttgaaccttt aggtattaaa
     1141 acctgttcct ttctttttat cataactatt aatatatcgt ctggtatatt agaatctatt
     1201 atacttttat tagcccattt attaccctca tcaacattta cttcgataag tttagagcta
     1261 atttctccat cataatcatt aaatgttttt aatacaggtg tattatcatc aactaaatcc
     1321 aactttctgg ctacagttgg tatcaatgaa ccttgtattc ctactgaaag caacgctata
     1381 aaaaatatta tatggaatat gtcattctct atggctactc cttgagtaac agcataaatt
     1441 gcaaaaacta ctgaagccgc acctctaaga cctacccacg atatgaataa tttttcttta
     1501 tttgagaatt taaattttgc tagaactata aatgttgcta atggtcttgc aataactatc
     1561 atgaatatag atatggccat tgctatacca aatacatttg gtaacttaga agggaaagat
     1621 aacaatccta acataaagaa taacattatt tgcataatcc atgaaattcc atcaaagaaa
     1681 tgaaccaaac tttttttaag aggtatccta ctatttccta taataatacc tgaaatgtaa
     1741 acacttaaat atccatttcc tccaagatac tcgctaaatg cataagaaag tattgcaata
     1801 gctgttataa atatagtata aaatccttct atctcgaagt ttgaatgtct taaaatataa
     1861 acagataatt ttgctagtat aaatcctata gctaacccaa aagctatttg ttttattacc
     1921 attggtgcaa taagttctac cccagaatta ttcatcaatg tcaaaagaat tagtgtcaac
     1981 atatacgcta ttgggtcatt acttccactt tcaacttcta ataatgatgc aagtgaaccc
     2041 tttaagttta gtttttgaga acgcaacaca gcaaacacag atgcagcatc tgttgaagct
     2101 gcaattgccc caattaataa accttcaagt aaactagttc ctattaccat gtggcaaaac
     2161 atacctgtaa ccccagccgt aattattaca cccagtgaag acattaatat agaaggtata
     2221 gcaacaggtt tagccatatt ccagtttgtt ccaaaccctc caaaaaacat tataaatatt
     2281 agtccaatag aacataattg ttttgttagt tcatagttat caaaatagat tcctaccatc
     2341 ccatcagacc caaataacat ccctagtaag ataaaaatca ataatattgg tactccaaat
     2401 ttatacaata ccttgctaga agttatacaa atcaatagta ctaacccact tataatcatt
     2461 aattctgtca tagcatcaac tccatttcat ataaattttg tagattaatt tacatctaca
     2521 attttattac ttattcaaat ataacatgac ataaatttta taagttatgg tacataaagt
     2581 tcaacaaaaa tttcacaatg cattaaaatg tgagtg
//
LOCUS       DAEMIK010000262         2525 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEMIK010000262 DAEMIK010000000
VERSION     DAEMIK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.267"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            461..940
                     /locus_tag="KPK87_003746"
     CDS             461..940
                     /locus_tag="KPK87_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2539847.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            937..1716
                     /locus_tag="KPK87_003747"
     CDS             937..1716
                     /locus_tag="KPK87_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539848.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMTNNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANDVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLIKMAHEVIDAK"
     gene            complement(1966..2466)
                     /locus_tag="KPK87_003748"
     CDS             complement(1966..2466)
                     /locus_tag="KPK87_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539849.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT          990 a          351 c          311 g          873 t
ORIGIN      
        1 ctctatctaa aataatgaca tttctaactc ttaattctca tttaagtttc attgtttttt
       61 cttaaaagcc tacttttagt caagatactg ttatctttac tcattattta aagaacttat
      121 tttaaatctg tacatgaagt aaataatcca aaagcattgt tattctaact actcaacatt
      181 aaatacttac tattttctaa ttactcaata taaagactta ctataaaact aaatacataa
      241 atggcttcta aagtttaata tatctacatg gctttatata tgacaggaaa aatactagaa
      301 aaaatatatt atagaatact tgtatataat aggcaaaact ttcaaactta aatttatcaa
      361 ataatcataa aaaaagtgaa ccatctatag taaaatttta tctaataagt gaaaggcaaa
      421 aaagtagcgc tacgatttgc atagaaggga aggaacttag ttggataaaa ctacatttac
      481 aaacaatata cttgaatcag aacagacttt gtatcgagta tctaagtcta ttctaggaaa
      541 cgaccaagac tgtgaagatg ctgtcaacaa tgctatttta aaagcatatg aaaagttgga
      601 ttctcttaag gaagaacaat actttaaaac atggctgatt agaatagtca taaatgaatg
      661 taattcatta agacgcaaac gattaaaatc tctatcattt gaagatgtct tcaaaaacaa
      721 aaaaattgat gaaaaagatg actatagcga tttgtatact gctattcaaa gtctacctaa
      781 aaaaattaaa attccaatag tcctttacta tattgaaggt tattcagtag atgaagttaa
      841 ggaaatatta gatatacctc aaggaactgt aaaaagtaga ttatcaagag gaagaagatt
      901 attaaaaact aaattagaaa acacggaggt aatcatatga ataaaaatat attaaataat
      961 tcgtttccag aagttccaga aagttttcat aatagtttaa gcaaaacact aaatagttta
     1021 ccagaaagag aggaaaatta taaaatgact aataataaaa cacacaaatt accatttaga
     1081 aaaggcttag ttgcaacttt agccattact ttgatattga gcacaacagc ccttgcagta
     1141 ggacatatat atagtttagt tggaaaatca agtactaagg ctacctatac atctatccca
     1201 actacagaac aagtaaaaaa agactttaac tttaccccta atatagtttc tgaatttagt
     1261 aatggatata aatttaaagg agcatatact gtaaataaaa aagcatttga caaagaaaat
     1321 aatgttttag gaaaatcaaa agcacttagt tttgaatata aaaatggcaa tgatgaaata
     1381 tctcttgaca cagcaaatga tgtacttggg aagagttctg acaatctaaa agttgtagat
     1441 acttacaaag atattaatat ttcttatgaa gcattcgcac aaaagtttgt accagaaggt
     1501 tataaaatga cagaacagga taaaattgat gaaaaatcag gcaaatacac atttagtgta
     1561 gggccaaata atgaatcaga caaagttgaa gttaataaat ttaagttttt gacatggaaa
     1621 caagatggag tttactattc ttttacagtt caagactcaa atttatctat gaatgactta
     1681 attaaaatgg ctcatgaagt aatagatgca aaataaacat aagtaataat aagttgttta
     1741 tgagtttatt atttatgact ttaatttttt tattagtaaa catggttgta tcttccatct
     1801 ttatattttc tttaaattta atatataaaa ttttagatat ttagctacct tcaaaagaac
     1861 cattattaaa aagcccttaa aaatatttct tttatatgga atttcaaggg ctttaacttt
     1921 attttataca agtgtatatg gaattttaaa gactttaact ttattttata taagtgtaag
     1981 ctccatttga attacttctt cttttacagg tctatcattc catgtaaaac cactagatat
     2041 tgtatttacc tctacaaacc ctaaagattt ataaacatga atagctctac tattactttt
     2101 aaatacatca agctcaattt tttcataccc atattctttt gataaatcaa taatttgttt
     2161 taaaagacta cttccgatac ctttttccct atactcttta agaataccca tgcctaaata
     2221 accaactttt tcagttttag gtaaaacgtc acaccaacca atacagttat ctgattctaa
     2281 atcaattata aacagttgtg gtaagttttt atttataata ttttttataa attcaactgt
     2341 ttcttcaaaa ggaaaggctt catccatagc tagatatttc ctctcttttg ctattttatc
     2401 aaaagtttgc caatacgaat aaatatactt ttcttctgcc tttataaatt ttatgccttc
     2461 tatcaactta acatttcccc tttttaattt ttgattgtag ttaacttatt ttatatgatt
     2521 ctatt
//
LOCUS       DAEMIK010000263         2508 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEMIK010000263 DAEMIK010000000
VERSION     DAEMIK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.268"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            256..2391
                     /gene="rnr"
                     /locus_tag="KPK87_003749"
     CDS             256..2391
                     /gene="rnr"
                     /locus_tag="KPK87_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG2539850.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1078 a          250 c          464 g          716 t
ORIGIN      
        1 atttaagcaa ttatgataaa gaataactta agtaattata atgatattta caaataattt
       61 ataaaacatc ataatttgtt tgaagttagt atgacaaagc ttacattaaa tcaaaaatat
      121 aaataagtaa ttttaacttg taataataat aaggaaatat agaaaattat gtgataagta
      181 aaaaatgttt tgtctgaaga aatataaaag acaatataaa taataatata atgaaaagta
      241 aaacaggagg gaaaaatgat accaggactt aaagaaagtt tgctaggatt aataaatgaa
      301 agtgcatata atcctctaaa aaaagaagaa ttagcagaaa tatttgatat acattcatca
      361 gaaatgccaa tgttttataa ttttttaaat gaattagaag aagatggtta tatctgcttt
      421 actaaaaaag gaaaaatagt atctcccaat caaatgggat actttgttgg aaaatttgta
      481 tcacacaaaa agggttttgg atttgtagaa tctgatgttg aatatacaca ggatttattt
      541 atctcaagtg acaatataaa tggtgctatg cataatgata gagttatggc agaaatagta
      601 gtacctgcaa ctgaagaaaa gagagcagaa ggaagaatag taaaagtaat aaagagagaa
      661 ataactagaa tagtaggtac tttccaatca agtaaaactt ttggatttgt aacaccagac
      721 aacaagaaat ttactaaaga tatatatata cctaaaaaac attttagtgg agcagtagat
      781 aatgataagg tagtatgtga aattacagta tggccacaag aggatagaaa accagaaggc
      841 aaaattatag agatactagg tcaaaaaggt gaaagaggcg tagaaataga ttctataata
      901 agagaacatg gtcttccaga agaatttcca aagaaagtat tgcaagaagc agaagctgta
      961 gctgttgaga ttcctcaaga agagataaaa agaagaagag atttaagaga tttaaacata
     1021 tttactatag atggagatga cgcaaaagac ttagatgatg ccatatcaat agaagtatta
     1081 cctaatggaa acttcaaact aggagttcac atagctgatg ttacacatta tgtaagagag
     1141 aaaaataaac ttgacaagga agcgttaaaa agagctacat cagtttattt agtagataaa
     1201 gtaattccaa tgttgccaaa aactctttca aatggagtat gtagcttaaa tccatttgaa
     1261 gataagttaa ctctttctat atttatggaa atagaccata aaggaaatgt ggttaaacat
     1321 gaaatatgtg aatctgtaat aaattcaaaa gctagaatga cttatacaga agtatctgat
     1381 atattagaaa aggatgatga aaaacttaaa aagacttttg aacatgttgt tgaagatttt
     1441 aaaaatgctg aaattttagc taggattctt atgtcaagaa gggaaaaaag aggagctata
     1501 gacttcaatt tcccagaggc aaagataatt ctaaatggaa aaggcgaagt tgtagatata
     1561 aagccatatg aaagaagaat atctaataag ataatagaag aatttatgct tataagtaat
     1621 gagacaattg ccgaacattt ttactggatg ggaataccat ttgtgtatag aatacatgag
     1681 acaccatctt tggaaaaaat ggaagaattg agtaagttta tatctacatt tggatatata
     1741 ataaaaggtg ataaagagga agtacatcca aaagcattac aaggtataat agagaaaata
     1801 aagggcaaaa aagaagaagg agctatcagt actataatgc ttcgttcttt aaaacaagct
     1861 aagtactcac cagaatgtag tggacatttt ggattagcag ctaaattcta ctgtcacttt
     1921 acttcaccaa taagaagata tcctgacctt caaattcata gaataataaa agaatcttta
     1981 aataataaaa taagtggaaa aagacaagaa cagcttacta ctatagtgga ttatgcatct
     2041 gttcaatcat ccgagaaaga aagaaaggca gagttagctg aaagagatgt acatgatttc
     2101 tataaggctc tttatatgaa ggataaagtt ggtcaagaat ttgagggtgt tgtatctagc
     2161 gtaacttcat ttggaatatt tgttgagcta gataatacta tagaaggatt gataagactt
     2221 gcaaatatga atgatgatta ctatatttat gatgagagtt cttatagtgt tttaggagaa
     2281 agaactaaga agtcatataa gataggagac cttgttaaga taaaggttga aagtgttaat
     2341 gtagatttta aagaaattga ctttgagata gttgaaaaaa tagaggagta gataaaacag
     2401 ctaaaataag agataacaag ttgtagtatt tatgatatgt ataaatatat agtgcacaat
     2461 actctataaa ataaatgata aaaataggta attgagaaat atctaatg
//
LOCUS       DAEMIK010000264         2475 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEMIK010000264 DAEMIK010000000
VERSION     DAEMIK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.269"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(67..675)
                     /locus_tag="KPK87_003750"
     CDS             complement(67..675)
                     /locus_tag="KPK87_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG2539851.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(784..>2475)
                     /locus_tag="KPK87_003751"
     CDS             complement(784..>2475)
                     /locus_tag="KPK87_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG2539852.1"
                     /translation="DKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYENI
                     ILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFFD
                     NREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFKL
                     LTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGIS
                     SDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKRS
                     RVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIEN
                     LYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIIDI
                     TERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGEI
                     LVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKSL
                     IEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
BASE COUNT          781 a          419 c          195 g         1080 t
ORIGIN      
        1 taaaaaaata agatacatac ctataaaata ccttcctata gatacatatc ttacctatat
       61 aaaaacttat tctatataaa aacgccataa aaaatctttt gcttcttctg cataatctat
      121 atttatacgt ttactagttt ttatatctaa ctcatcctta ctaaatactt tttttccttt
      181 atcatcataa taaaaatcac ttatatataa ttcttcaccc attatactct tactatttag
      241 acttctatct atttttaaag ctttgcaaag ttttcctggt ccgttagtaa tattttttac
      301 ttgatattta ctcaactcag tgtaagcttt gttgtatcta ttcatagata tctcatctaa
      361 agaagttata ggctctaccc ctcttattaa tacacactct ggcacatttt ctatatttgc
      421 agataaattc aagcaattat acataccata aattaaataa acatatatat gtccaccatc
      481 taaatataat ggcttagttc tatctgtctt tttattgcca tatacatgtg ctcctttatc
      541 atttacaccc atgtatgctt ctgtctctat gattttggta acaattactt tattttcata
      601 tttccttatt agatatttac ctaatattga tttggctaaa tcaattccat tttttctaaa
      661 aaaatccttt tccaaaattt cacctcataa aatttattta aaacgcatca aatacaacta
      721 aagtaataaa taaaattaac tcttattaat gtgatatata taaacaataa atttagacat
      781 ttattattta tatatatcaa aaaattctat ctcaaacttt tcaatattat cattgtaatt
      841 tgaattatca tatacttctt caacaacctc tttaattggg aatataactt ttacattagt
      901 tccttctcct accttactat ctatgtaaat cttcccattt tgaatttcaa tcaatgattt
      961 tgtaaggcat aaacctatac cacttccaaa ttcatttgat ataccactgt tttcaatttg
     1021 tttaaatcga ttaaatatat catttaattt ttctttgtct atgcccactc cagtatcttt
     1081 tataattatc tctactgttt cgtctttttt atatatacct acaaggattt ctcctccttc
     1141 taaagtaaat tttatagcat ttgataatat atttagtatt attctttcta tcgattctgg
     1201 gtcaagccct accataacag tttcttcatc tgtatcaaaa attaaatcta ttcctttaga
     1261 cttagcataa ctactaatag atgtaactat tctttctgtt atgtcaataa tatcaaaatt
     1321 aactgcttta atcttatata tgtctgactt aacttgagaa atatttatta tattatctat
     1381 taatctcaat aatctaaaca tattttgttt agttattttt atatgttctt ttagtatatc
     1441 ctgaaaatca attatttttc cttttttata aaggttttca ataagttgaa ctgaactata
     1501 tatattattt aaaggtgtct taagttcatg agatatatta gcataaaact ccatcttcat
     1561 ttggtcatac tgtctttgtt ttattaatgc ttttttaagt ctttcagact ctctttgcac
     1621 acttaaatct ttaataattc tcactctact tcttttctgt ccaaagtata atgttcttga
     1681 tatcattttt acttctttaa tgtgattatt ttcatttact atatggatac tcttctctct
     1741 ttctcctata tttaaattgt ttatgtattt ttttactaat atatttctat atttttgtgt
     1801 tgtaactcct aatacatcac tagaaattcc gaacaactct ctaaatgctc tatttgtata
     1861 tagtactctt gatgtactta aatcctcaag aaaaatagcc tctggtataa catccagtaa
     1921 tttttcatat attatttttt gttcatttac tgttctaaga atatttatct ctttagttat
     1981 atctctaaat gtaagcaatt taaagagttt attatctgac caatagtctc caactctaac
     2041 ttcttcaaca tgctcttcac cttctgagtc agacatcaca aaattaacta tacagtttgc
     2101 tgtgtcattg taacttacta tttcagcttt cttttcttgt gttagattta atcttttaaa
     2161 ctccctattg tcaaagaaat ctatgttaaa agtttcaaaa tattgttcta atgtcatatc
     2221 gactaaatca cttgaaacac atttaaaact cttttcatat tttttattta tacttattac
     2281 cttattcttc tttatgccac cttcagcact tcttacaagt ataggagatg gaatatttag
     2341 tataatgttt tcatagtctt tatttatatt tcttatttct tcatctattt tgatttcatt
     2401 tgtaatatca tttaaactta ttaagtaatt gctttcatct ttattattac ttaaacttaa
     2461 caaataaatt ttatc
//
LOCUS       DAEMIK010000265         2475 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEMIK010000265 DAEMIK010000000
VERSION     DAEMIK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.270"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(182..937)
                     /locus_tag="KPK87_003752"
     CDS             complement(182..937)
                     /locus_tag="KPK87_003752"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG2539853.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1377..2366
                     /locus_tag="KPK87_003753"
     CDS             1377..2366
                     /locus_tag="KPK87_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG2539854.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          904 a          309 c          367 g          895 t
ORIGIN      
        1 taaaataata aacaatataa ttccatatac caataaataa gataaccttt aagaaaaatg
       61 attatattta aaaggtaagc ataaaattta taaactttta gaatagaaat ctcaatcaat
      121 tagatattca aattgatgaa tatgaatacc taaatagatt gagatttatt atgtacaaaa
      181 cttatcctaa tatatctgac attttataaa gcccagattg cttaccaatt aaaaatttag
      241 ctgcaactat agaaccgttt gcaaatactt catcagattg agaatgatgg cttaatgtaa
      301 gaacttcatt atcacctgca aatataacat catggtcgct gattatattg ccacctctta
      361 tagagtgtat tccaatttca tttggttttc ttttacaatc tctaccatat ctaccatgtg
      421 tattttcact ttcaggcaag acttctctaa ttgcattagc tatcatagta gcagttccac
      481 taggggcatc ttcttttcta ttgtgatgtt tttctatgat ttcaatatca aaaccttcta
      541 acatctttga agcatcctta actaatttta ttaatatatt cactccaaga gatgtatttg
      601 atgataataa tataggaatg ttttttgatg cttcttctat tttatttaag tcattttcat
      661 catatccagt agtagctaat actacaggtg tattattttt tagagcatat gacaatattg
      721 gctctaaagt ctcatgatgt gaaaagtcta ttattacatc agttttttca gttacatcag
      781 tcattgtttt gtaaagcttt gtatcactat gatgaccttt ttcatcagca gacctagcta
      841 caccacaaac caactctaat tcattatctt tttttacaca ttctgctaaa actcttccca
      901 ttgttccatt atagccacta attataactt tcaacataaa ttcctcctat gaaattaata
      961 aaaatttgtt atatttctat aagtttattt tatcactaat ataatttata taaaataata
     1021 ttagtgataa taagcttaca atttatctta cattaaaaaa tttaagtgga tttttcaaaa
     1081 tatagataaa tttctttttc attgtttaaa tgataaaaat atatttgact tcataccaaa
     1141 aagagtgccc catgaactaa aaagttaatt ttgaaatacc atattcatat atcatatgat
     1201 ttatttggaa attaatatta atacattaaa ataactaaat acatgtatgc ataaagatgt
     1261 ataaaaaata tataaacata tagtattaat tttaaattca acaatttaat tgttaaaagt
     1321 gttattatta aattagaaat atcttaactt aatatactga gatgggagtg atagatatgt
     1381 taaaagaaga aaaaacttat caagagaaag acgcaaaagt attcaatgga gtggcagctt
     1441 tgataatgat tttattattt ttagtagttt caataggtat catggttttt gggatagtaa
     1501 aacttgatag tggatttact ggaataggtt cacttatgtt aattttggga cttgtattta
     1561 tagtagtaga ttttatccta ttttttgggc taaaaatgat taatcctaaa gaagctatag
     1621 ttctagtttt gtttggtaac tattatggaa ctataaaaaa agaagggtac tattgggtga
     1681 atccattttg ttcagcaata aacccagcag tatcaagaat taatgtatcg aaatcttcat
     1741 ctgatgaaaa ggtagatata tcaactaatt caaggggcaa aaaagtatct ttaaaaacaa
     1801 tgactcttaa taatgaaaag cagaaagtca atgatttatt gggaaatcca attataatag
     1861 gagtggttgt aatttggaag gttatagatg caacaaaggc agtatttaat gtggataatt
     1921 ataatacatt cttatctatt cagtgtgatt ctactattcg taatgtttca agactttatc
     1981 catatgatgt atctgaagat ggagatgaaa aatctcttcg tggtagtagt caggaaatag
     2041 cagatagatt aaaagatgaa ttacaatcaa gggttgacat tgcaggaatt gaagtgtgtg
     2101 aagtgagaat tactcatctt tcttatgcac cagagattgc agcagcaatg ttacaacgtc
     2161 aacaagcaga ggctataata gcagcacgta aaaagattgt tgagggggct gtaagtatgg
     2221 tagagatggc attgactaat ttaagtgaaa ataatgttgt gactttggac gaagaacgta
     2281 aagcagctat ggtaagtaat ctgttagttg tgctttgtgg caataaagat gcacaaccag
     2341 tggtaaacag cagttctatt tattaaatta taaaaagttt aatagtttat tttattgata
     2401 tatagaaaat ttagtagttc attttattaa attatagaaa gattaatggt ttgctttatt
     2461 gaattataaa aagtt
//
LOCUS       DAEMIK010000266         2472 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEMIK010000266 DAEMIK010000000
VERSION     DAEMIK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.271"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            274..>2472
                     /locus_tag="KPK87_003754"
     CDS             274..>2472
                     /locus_tag="KPK87_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
                     /protein_id="HBG2539855.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAEITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSGGSSGGSSSSKVGQIKFSATDKQIKMASGDKTVDPSDNTYILKLTNGTVKDGDVKA
                     NVKVAGLPSGLDYTAVGNRSANTITITVSGTASQSIQSDLNNVSVLVKAGAVSGTGAT
                     DSIANTTFEIKKYVAPAIGQLTFDATDKQIKMASGDKTVDPSDNTYVLTLTKGTAKDG
                     DVKANVEVTGLPSGLDYTAEGNRSANTITITVSGTANQAVQTDLDNVSVLV"
BASE COUNT         1074 a          315 c          451 g          632 t
ORIGIN      
        1 tttttataca aaaccctatt ttttataaca attcattaac ataaaaatca aacttacctt
       61 aaaatataaa tttacaaatt tttcaatcaa taagaaaatt tctttattcc ttacataatg
      121 actgcaatgt aacaaaataa ttacaaatta attatgaaat cattatagtt agaaacctct
      181 ttaaaattag atattaagta tcctttagaa ttagaacggg aacaaagtta aaaaattaag
      241 taatatcaaa tggacaaggg ggaagaaaat aaaatgaaaa taccaaagaa gattgctgca
      301 atgcttacag ttacaatgat agctggaagt tcaacagcag gcatagctag tgcacaaact
      361 gtggcaacaa atttaacagg gcaagaaaga tatgaaactg ctgttaaaat aagtcaagat
      421 ggatggaaaa atgctgatga ggtagtaatc gtaaacgact catctatagc agatgctcta
      481 tctgcaacac catttgcaaa agcaaaaaat gctccaatac ttttaacaag taaagataaa
      541 ttaaatgata agacgaaagc agaaatacaa agattaaaag ctaaaaaagt atacttaata
      601 ggtggaactt ctgtattaag tacaaatata gaaaaagaaa taaaagattt aaaaatatct
      661 tttgagagaa tctctggagc tgagagatat caaacatcct tagaattagc aaagaaatta
      721 gatgctataa gtgatgttaa gaaaatagct gtagttaatg gtgaaaaagg acttgcagat
      781 gctgtaagtg taggagcacc agctgcacaa aacaatatgc cagtaatact tgctgattct
      841 aaaaatggaa cagcagttgc agacaaattt ataaaggatg ctgaaataac tcaatcttat
      901 gtagtaggtg gagagagttc aatttctgaa gctgttaaaa ataaactacc aaatagtaca
      961 agactaggtg gaacagatag aaatgataca aatgcaaaag taataaaaga attttataaa
     1021 aagacagatt taaaaaatgc ttatgtaact aaagatggta tgaataaaca agaccaactt
     1081 atagatgcac tagcagtagg tgtattaggt gctaaaaatc aatcaccagt agttttagtt
     1141 ggaaagaact tatcagcttc acaaaaatct ctagttaact ctaagagttt tgataagata
     1201 actaaagttg gtgggaatgg taatgagaca gcatttaatg aaatgaagtc attacaagaa
     1261 gtaaaaacag ttgaagctaa aacaatttct gaattaaaat cagctataga taaagctact
     1321 gctaatgatg ttataaactt taagccaact agtgaggtta aagaagcatt tacaatacaa
     1381 actgataaag cagtaacagt aaacttaaat ggtacatata caaagactgt aactatcaac
     1441 atgcctaatg gagatgtaaa taactatgca aaagtggatg atgtagttat agatgatgta
     1501 aaagatggaa cttttgttaa ctacggaaaa ataactaact taaaggttaa tgataaaaat
     1561 ggagccaaga tagaaaataa ttctaaaggt gagattggta gtttaacagt agcatctgga
     1621 gcaagtcaag taaaagtgac taatggtggt aagataacta cagttacaaa taattctaaa
     1681 gggactacta tagataataa aggtacaata agtagtgtta agggagataa ttctccaaca
     1741 ataagtggaa atagtccaag ttctaactct tcaggaggaa gttcatcaag tggaggttct
     1801 tcaggaggaa gtagtggagg aagttcttca tcaaaggtag gtcaaataaa atttagtgca
     1861 acagataaac aaataaaaat ggcatcaggt gataagacag tagatccatc agataacaca
     1921 tatatactaa aattaacaaa tgggacagta aaagatggag atgtaaaagc taatgttaaa
     1981 gtagcaggat taccatcagg actagattat acagcagtag gaaatagatc agcaaataca
     2041 ataacaatta cagtatcagg aacagcaagc caatcaatac aaagtgattt aaataatgta
     2101 agtgtgctag ttaaagcagg agcagtatct ggaacaggag ctacagattc aattgcaaat
     2161 acaacatttg aaattaaaaa atatgtggca ccagcaatag gtcaattaac ttttgatgca
     2221 acagataaac aaataaaaat ggcatcagga gataagacag tagacccatc agataacaca
     2281 tatgtactaa cattaacaaa aggaacagca aaagatggag atgtaaaagc taatgttgaa
     2341 gtaacaggat taccatcagg gttagattat acagcagaag gaaatagatc agcaaataca
     2401 ataacaataa cagtatcagg aacagcaaac caagcagtac aaactgattt agataatgta
     2461 agtgtgttag tt
//
LOCUS       DAEMIK010000267         2463 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEMIK010000267 DAEMIK010000000
VERSION     DAEMIK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.272"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(325..1617)
                     /gene="brnQ"
                     /locus_tag="KPK87_003755"
     CDS             complement(325..1617)
                     /gene="brnQ"
                     /locus_tag="KPK87_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2539856.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALASSVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          833 a          384 c          253 g          993 t
ORIGIN      
        1 ttttttcatt ttaactttga ttaattaata agtatttaga aaataaattc tttatttttt
       61 ctttacaaaa atatgtattg atgtttttat attaatacta aacatttata atatataatt
      121 taattttatt tataattata aatgaatctt ttttatttta ttatctttat aaatcatact
      181 acttcaaaaa taaattttta aatttatcaa ataggattaa taatgttctt ttatctacaa
      241 taatttaata attcttttaa atataaaaag gaattgccta tataatttaa aggcaattcc
      301 ataattatca attagttttt attattatgc agttttactg cttttagatg gaataaagtt
      361 tcctattatt cctgctatta caactggaac tatccagtta aatcctaatg aagcaaatgg
      421 taagctatta actagaggaa tttttatacc aaaacttgtt gcaactgtta atacacttac
      481 taataatgcc atgtatgcag cacatttgaa cacgttgtca tttttaattt tattactaaa
      541 taatgctagt ataactagag ttatagttgc tggatatacc atacttagta taggagaaga
      601 gaattgtatt attgtactta ctccaaagtt tgatatgatt gcactaaata cacaaactac
      661 tataactata tgctcatact ttaattttcc atctgtaagt gttgagaaat attgaccagc
      721 tgctgaagaa agacctatag ctgttgttaa acatgctaat gcaactataa tagccaataa
      781 aattttacct gtagttccaa gtaaagctgc tgttattgca actattaaag ctgtttgttg
      841 aacatcttgt ccatattgtt gagacacagt tgctccaaga taacataatc caccataaac
      901 taatgccatt cctattactg caattacacc tgcacttatt gtggctttca tcttttcttt
      961 ctcacctgta taacctttag ctattactga catgattata attgatgcaa atacactaga
     1021 tgccaatgca tccattgttt gataaccttg agttatacct tctgcaaata cattttgtat
     1081 taaagaactg tctcttattt ctcctaatgg agatacgata cctttaacta ttaatactgc
     1141 taaagctata agtaaagctg gagttaatat tgacccaact atatctacaa cttttgatgg
     1201 tcttattgtt aaaaccaata ctagagcaaa gaatactata gagaatatta ctgggcttat
     1261 tccagaacct attgtaggta atatacccat ttcataagtt gttgctgctg ttctaggtat
     1321 tgctaaaagt ggccctatac agattaccat tgcacatcct aatgcaatac ttaatccttt
     1381 gcctgctctt gagaacatct tcgatgtgtc tccattgaac ttagttgctg ctataactgc
     1441 taataatgcc aaaccaccat ctgcaaaaag gaatcctatg aaagctacta gccaactact
     1501 tccagataaa actcctagat atggagggaa gattaagttc cctgcaccaa aaaacattgc
     1561 aaatagtgca aatccaatta caattacatc tttaaaattg atttgtttct cacccattta
     1621 aagcaccccc atttgaattt tactaccttt tactaaaaaa taagtccaat ctcttaatta
     1681 aatgaaaacc ttttgacgag attttattta attatacatt tttgacttaa atttgtcaat
     1741 tatgtttcat aatttataaa atcttcacaa tatttaatac aactttctta attaaacaaa
     1801 atcaacctgt atactttaaa catagttggt tttttgtttt ttctctaatt tttcttgata
     1861 aaattaacta ttccgaataa ttaaaatatt aagacttttt agattattcg atttattatt
     1921 aagttgtatt gctttatata ctgtatatat cactctcctt ttttacattc atataatctt
     1981 tttaattcaa tttaaatttt aaatattttg tgtttattat ataatctgaa aaaaaagcag
     2041 tcaatagcat ttttaatttt tttaattttc aaaattatga ttcttaaaaa atcgttttcc
     2101 ttttatcata acacctattt acgttaattc ttgtagtatt ttgtatttat tttgctaaat
     2161 tgacagttaa ctttattcaa tttttattct acttacgtag atttacctct tgttttttac
     2221 tcttaaaacc attttttctg aaaattcctt attatgattt atcaacttat ttttcttcat
     2281 aacaattact ctctaacttc caacttactt aaactttgca gttgctagta ttcacgttta
     2341 catacttata tgtcataatt aactaaaaat tcttaatata tctctcaatg aaaacaattt
     2401 actcatttat ttaatacatt tatataatca tatgtcttaa attttgtttt attattttat
     2461 tat
//
LOCUS       DAEMIK010000268         2453 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEMIK010000268 DAEMIK010000000
VERSION     DAEMIK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.273"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            457..726
                     /locus_tag="KPK87_003756"
     CDS             457..726
                     /locus_tag="KPK87_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2539857.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(944..1189)
                     /locus_tag="KPK87_003757"
     CDS             complement(944..1189)
                     /locus_tag="KPK87_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG2539858.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            1615..2313
                     /gene="tcdC"
                     /locus_tag="KPK87_003758"
     CDS             1615..2313
                     /gene="tcdC"
                     /locus_tag="KPK87_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG2539859.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT          880 a          274 c          382 g          917 t
ORIGIN      
        1 gaatatttga ctcttggaat gtgtaattaa attaagtttg gttattcgat ggaaggcaaa
       61 tttgatataa aactccattt aaagtgccag ataaaaactt tatggttata taaaagttaa
      121 aagtaattta tagtgttata gaacattcaa agggatttaa agtaatctat ggtgttttga
      181 aatattcaaa gggatttaaa ctaatctata gtcttgtaga atattcaaag agatttaaag
      241 caatctataa tgctgtaaaa tatattcaag gggatttgaa gtaatttatt gtgttttgaa
      301 atattcaaaa agatttttgg aatagttttt ataaatttac cagtactaat aactttatgt
      361 ttaacattta gatgacctaa aatcaactat ttgcactttt gtattcatct aaaatcattt
      421 tataatattt ttaaataatg gaaaggatgt ttgaacatgt ttgaatcaag atgtggtgtc
      481 tgttgtgaca gttgtacaaa aaaagaacaa gtaaattgca ctggttgccc aactatggaa
      541 aaaccatttt ggggtggaga atgtaaggtt aaaacttgct gtgaaagtaa ggaacttaat
      601 cattgtggtg aatgtgatac tttcccttgt gatatgcttt taaataaggg aaaagaccaa
      661 ggttttgacc ctatggtaaa tattgagcaa tgtagaaaat ggcttgaaga aacagtttct
      721 aattaattgt tttaaaatgc tttgttaagt aatttattta atcctttgtt aggtagtatg
      781 tttagtcatt tgttgattgg tatatttata catttattga tgggtatatt tagccataca
      841 aatagattta cataaaattt acgactaaaa aagagttgag tattagtttt tattatttaa
      901 aacggatatt caactctttt ttatgtctaa cttttttgat tttttaatct ttccaatacc
      961 accactttaa tttttctggt tctggatttt cagttgatgc aaaatatact gcacatctgt
     1021 atacatataa ctgacatctg tctatgtgct gtccacgcat taaacattcc ttttcataca
     1081 tttgtgctgg gtttgcatct ttaagtgatt taatagttgt atatcctaac atgattaaag
     1141 atttttctgt tgctttccct acccctggaa ttttttgtag ttctcccata cttcaccttc
     1201 tttctggtat attcttttta tattatacct attaccagat atttttttat tatagttaat
     1261 atttaatatt taatttttta ttatatcaat ttatttatgc tctttcatat atatatattt
     1321 taccacctct aaatcactga gtcacttaat tacatcataa tatagtttta tacaaataaa
     1381 atactttatg tttaatttaa tatataaaat tcaccttcaa gaaaattata ttataatctg
     1441 acatttttat cttatttttt caaaatatat tgaatcttct cactttattt tgtaaaatta
     1501 tgcttagggg aaatatattt taggaaaata tgaatatata atttttagtc aactagttat
     1561 tttaagtttt taacttttaa aataaaatat atctaataaa agggagattg tattatgttt
     1621 tctaaaaaaa atgagggtaa cgaatttagt aatgaaggaa aaggaagctc taagaaaata
     1681 attaaattct ttaagagcac aaagggtatt gctctactgg catttatttt aggcgtgttt
     1741 tttggcaata tatcctcacc agcttgttct gaagaccatg aggaggtcat ttctaaccaa
     1801 acatcagtta tagattctca aaaaacagaa atagaaactt taaatagcaa attgtctgat
     1861 gctgaaccat ggttcaaaat gaaagacgac gaaaagaaag ctattgaagc tgaaaatcaa
     1921 cgtaaagctg aagaagctaa aaaagctgaa gaagctaaaa aggctgaaga acaacgcaaa
     1981 aaagaagaag aggagaagaa aggatatgat actggtatta cttatgacca attagctaga
     2041 acacctgatg attataagta caaaaaggta aaatttgaag gtaaggttat tcaagttatt
     2101 gaagatggtg atgaggtgca aataagatta gctgtgtctg gaaattatga taaggttgta
     2161 ctatgtagtt ataaaaaatc aataactcct tcaagagtat tagaggatga ttacataact
     2221 ataagaggta taagtgctgg aactataact tatgaatcaa ctatgggtgg aaatataact
     2281 ataccaggga tagctgtaga gaaaattaat taatattgtt ttgtattata gttaatattt
     2341 tatattatag tcaatatgtt taaagatgtt tttataattg caaataaaca gttacaaggc
     2401 tctaaattag tttttgcttt tagcatatta tctatttttt atcaactatt aat
//
LOCUS       DAEMIK010000269         2424 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEMIK010000269 DAEMIK010000000
VERSION     DAEMIK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.274"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..696)
                     /locus_tag="KPK87_003759"
     CDS             complement(<1..696)
                     /locus_tag="KPK87_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2539860.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDI"
     gene            complement(736..1083)
                     /locus_tag="KPK87_003760"
     CDS             complement(736..1083)
                     /locus_tag="KPK87_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539861.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1301..2257)
                     /locus_tag="KPK87_003761"
     CDS             complement(1301..2257)
                     /locus_tag="KPK87_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG2539862.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPIRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT          820 a          363 c          282 g          959 t
ORIGIN      
        1 tatatcttct ggagttctat caaatttgag ttcaaaattt tcatcttcaa agtagggaat
       61 ataatataaa ggtgttttga gagcaggtaa tttattatat aattcttcaa gaatatcgat
      121 atttttaggg gagttaaaga aaaagcattt acatacattc attaaatcca cagcatctga
      181 aagtgagtta tgtttaatat ctccttctac agaaaaaatt tttttcatat tcagtaaaga
      241 tatttgctta gataagattc tattattgta tgtaatatgt tgagatataa aaggttgaat
      301 gtcaatcatc atattaaata actctaagca tttacctgta tatttatata ctctaaatga
      361 agttagaagg ccagttatat caaaatcacc ccaattatat attgtaacat catcaaattt
      421 acaaaaccat ttataaaaat catccataac atataaaaga ttaggagcat tattgatatc
      481 tagttggttc aatttggtta aatttttaca tttatttgaa agtattttat tgtattttgg
      541 cttaactaaa gagtaataat tatctattgt attaaattcg ttatcaacta aaactccacc
      601 aatagaaatt aattccgcac cattatattc ttttggttta tttttattat catctagtat
      661 attaaattca aagtctataa ataacttgta tttcataaat ctaaagcaca tcctcaggta
      721 aattatttat tgagcctaag catcataaga atcaatagat ataatgactc taaacatcaa
      781 aggttcatta caacttttat ttatttcata atttaattca aataaatctt ttaagatata
      841 atttataagt attctttccc aacccatttt taaattaact ttagagtcta ttaatatatt
      901 ttctatcttt ttaaatattt ctatatttgc tggtattata ctgagctcaa tattactttt
      961 aaacgggtta gactgtaaat ctgttataag tatatttttt aattcctctg taagttcaga
     1021 aattaaacta tcacctatgt atttttttat gatttcctct ctaatttctt ttaaatttag
     1081 caatattagt acctgctact tacatagtag cttttccttt ccttttaata ctattattca
     1141 tttttgaatc ttatttagat atattttaag attaaaagtc tttagctata taatttttta
     1201 cccattttta acttatatta acatattaca ggcttatatc tccaccctgt ttacattgag
     1261 gatggagtat attgcctatt ttgataaaaa tgataggttg ctatttgcct attccttttt
     1321 ctgataattt ttctaacata tctttggtca ttttatctaa atcataacta aacttaaagt
     1381 tccattcttt tactgcacta ctactgtcta gagagttagg ccaagaatca gctatacttt
     1441 gtcttatagg gtctacatca tactctatca caaaatcagg aatatatttt tttatagaat
     1501 cagctatttc ttcgggagaa aagctcatag cagtaatatt aaaagcattt ctatggatga
     1561 gtttatcagc tggagcttcg attaaatcca ctattgattg aagtgcatca ggcatataca
     1621 tcatatccat ttttgtacct tctgctatgt aagatttata tctcttattt tttaatgcct
     1681 cataatatat atcaactgca taatctgtag ttcctccacc aggaggcgta acataagaaa
     1741 taagacctgg aaatctaaca cctctagtgt ctactccaaa ttttgagtga taataatcac
     1801 atagtaattc tccagaaact ttagtcacac catacatagt attaggtctt tgtaatgtat
     1861 cttgaggagt catatttttt ggagaatttt caccaaaagc agcaattgaa ctaggtgtaa
     1921 aaaatttaca atccaattct ttggctattt caagaccatt tattaatcca ttcatattta
     1981 agttccaagc ttctagaggt ttactttcag caactgcaga aagtaaagat gctagatgaa
     2041 ttatagtatc tacatcatat ttttttgcaa tttcaaaaaa acgattctta tctgtaacat
     2101 ctaatatctc aaatatacca gattcacata ctggattacc ttcttttact ctccttgaac
     2161 tagcgattac attttgttct ccatactcat ttctaagctt tattgttaat tctgaaccta
     2221 tttgtcctaa tgcacctgtt ataagtattt ttttcaattt tacaacctcc taagggttta
     2281 atttatgtag tactacttat ttttatttta gcaaatcttt tgtttaaata caaagaatta
     2341 aagataataa tatacgactt aattgaatga agtgcagtaa gtgcctaaaa atttaaagct
     2401 aatatataat ggtatattgt ataa
//
LOCUS       DAEMIK010000270         2405 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEMIK010000270 DAEMIK010000000
VERSION     DAEMIK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.275"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            349..1875
                     /locus_tag="KPK87_003762"
     CDS             349..1875
                     /locus_tag="KPK87_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2539863.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKVAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1903..>2405
                     /locus_tag="KPK87_003763"
     CDS             1903..>2405
                     /locus_tag="KPK87_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539864.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDS"
BASE COUNT          945 a          206 c          434 g          820 t
ORIGIN      
        1 ttaagtaaat atttttatag agagattgtt atattacata tcataaaaat attaaactaa
       61 ctaaatcaat aaaaaagttg gtggctaaca atttataatt gttagcacaa catcatctta
      121 tgtattgaaa tctaaaatat cctcattttt acttagcata aatactatta attaagaaaa
      181 taatatagaa attataaata tatataagtt tagtctggtg tataaaacaa gaaaactaag
      241 tgatttttaa tttagctttt tattaagctt attttttata ctatttataa taaataaact
      301 ccatgatggg aaaactatta ctatttaata tgtaaaggag tattttaaat gaatgaatca
      361 tattggtttt taaactcaag tccaaaagaa tataacaaat taggtgaaaa tataaaaaca
      421 gattgtctta tagtaggtgg tggaataact ggattgacaa ctgcatattt attagcaaaa
      481 gaaggtaaaa aagtagtgct tgttgaggct gataaaatag gatatggaac ttctggaagg
      541 aatactggta aggtaacttg tcagcatgat atattttact ctaagataga gaaaaaatat
      601 ggattagata aagcaaaatc atattataat gcaaataatg aagctttaaa tttagttgaa
      661 caaataatag aagaaaacaa tataaaatgt gattttaaaa gggaaacttc ttttgtattt
      721 acagaaaaag aggatactat aaagaatatt aaagatgaat atcgtacatg taaaaaaata
      781 ggaataaatt gtgagtatca tgaaactata gaaaatattc cattagatat aaagggggct
      841 attagtttta ctaatcaagg tcaatttaac ccaaagaagt atattgatgg attggcaaaa
      901 gtagcagtaa atttagggct aaaaatatat gaaaatacac cagtagtaga ccttgaaaaa
      961 ggaaaaatat gtagggttaa aacaagagaa gataatataa tagaagctga gaatgttata
     1021 atttcatctc atagtccatg gtatgatgga ttgaattttt attttgcaaa agaatatgca
     1081 gagcgtgctt atttaatggt tgcagttttg gaaaataaat tagcagatgg tatgtttata
     1141 agtatagatg acccatcaat aacttttaga caatataatg atggaagtga aaatttactg
     1201 atttttggag gaggagacca taaagttggt cagggaggaa ctgagaaaga aatatttgat
     1261 gatttagagc attatggtaa agaagtcttt aaggtaaaag attttaaggg gaaatggtct
     1321 gctcaagata atatgagttt tgacaatgtt ccttatattg gttatatcaa caaaagagaa
     1381 gacaatattt atgtagctac agggttctct aaatggggaa ttactaatgg aactgctgct
     1441 ggaataataa taaaagattt gataataaat aataattctg attataaaga tacttttaat
     1501 ccatccagat taggtagcta cttttcaaaa gattttataa aggaaaatgc aaacgtagca
     1561 ataaattatg taagtggaaa attaaaaata ggtagtggag atatgcctaa aaataatgga
     1621 gaaggaaaga ttgttaatat tgatggaaaa agatatggtg tatataagga tgataatgga
     1681 gatttttata ttgtagacac gacatgtact catttaggat gtgaacttaa ttttaacagt
     1741 gaagaaaaaa catgggactg tccatgtcat ggttctagat ttgactataa gggaaatata
     1801 cttgaaggac cagctcttaa gccattaaaa ctttatggac atggtgataa tgatgttaat
     1861 cctaagttat tataaaatta tttttaggaa ggtaaagtgt atatgaataa acttttaaag
     1921 ccaataacta tactattgta tatagtttat attttatctg ccgtattttt tgtagcaaac
     1981 aaagaatatg gaagtctagg tctagtagca attagtttag ttggtacttt cattttgagt
     2041 tttataaata agcaaactga aagattatta agtaaagaat tatatataat gcttgcttta
     2101 tttataatgt tttcatcatt gttaggtact tgttttaatt tttatgatat aaattattat
     2161 gatgattttt tgcatttatg gtcaggtata atatcttgta ttgtggcttt ttctataatt
     2221 aaatactttt atacacaaag agagataagt aaaatgagta aagtgttttt agttatattt
     2281 ttatttatgt ttagtatggg agtagcgagt ttatgggaaa taggtgaatt tttaatggat
     2341 acttttgttg ggactactac acaggccgga gggttagaag atactatgat tgatatggta
     2401 gattc
//
LOCUS       DAEMIK010000271         2384 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEMIK010000271 DAEMIK010000000
VERSION     DAEMIK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.276"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(497..1048)
                     /locus_tag="KPK87_003764"
     CDS             complement(497..1048)
                     /locus_tag="KPK87_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539865.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1239..2201
                     /locus_tag="KPK87_003765"
     CDS             1239..2201
                     /locus_tag="KPK87_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG2539866.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          918 a          257 c          399 g          810 t
ORIGIN      
        1 tgtgttattt gtagtaaata taaagtgcat atagtgataa aacaaaagtt tcatataaat
       61 gaagaaaaaa atataaaata atttatttaa ctacttaaaa ataaactaaa agggataata
      121 aaatagcttt taggttatgg taataaaggt atttaaaaag agtgcataag cactccaatt
      181 gtgagaaata catacacaaa tcattattac aacaatgaaa aatgtacata taagagacgt
      241 tttgtatttg ttttaagtga taatatttta atactaacta aataattatc acaatagtaa
      301 aaaagaaaaa ggagtatttt gtactccatt tgtcaaaaat ataaaacttt tatataagct
      361 tattataaca taattgataa aagtgtaaag atatataatg aatttataaa gaaaaataaa
      421 ataaaaaaga taaatcctaa tcaaaggatt tatctttttg tatagaaaat aattattcta
      481 ttattaaatg gcatggctaa actggataat aatttattat tactgctttc aatgtttttt
      541 ctccttgatt atggtatgta tgaattgatt cagctgaaaa acaaatagag tcattttctc
      601 taagttgata aatagagtca tttacttcta attttaattg accttctata atcataatat
      661 attcctgtga tttttcagaa tgaccaacag atgtataact atgaccttct tctaattcca
      721 tttgaaataa ttcgaagttt ctagttggtg tattgggata ataacaataa agacgataat
      781 ctttattttc tgaaacttgt acatctatat cagtttttga tacaataaaa gtttcatttt
      841 gaggttgttc cagtattgca gtatatggaa catttagtcc acttgcaatt ttccaaattg
      901 tgtttactgt tgggtttgag tctccctttt caatttgcga aagcattact ttacttacgc
      961 cagataattc agcaagttga cctagactta agttacgttc attacgtaat ctatttaaat
     1021 ttttagcaat aacagtatta atactcatag tagctcctta aaatgtaaat tattgacaaa
     1081 tataatttac gaatgtataa tttatttaac attatttata atttacaaat gtataatata
     1141 atgaacgtta aatacactgt acatccgtaa tataaattat actgcataaa gagttgtttt
     1201 tcaacggatg atagttgaga gattaagggg gaattgtaat gcgtgtactt aataatgtta
     1261 cagaggctat aggcaatact catatgttac atttatccaa aatagcagat gaatttggag
     1321 ttcaaggtaa tatttatgca aagatggaat acttaaatcc gggatttagt aaaaaggacc
     1381 gtgtagcatt acaaatgatt accgaagcag aaaatgcagg tttattagtt ccaggtcaag
     1441 cagttgtgga attaacaagt ggtaatactg gaacaggact agcaatagct tgtgcatgta
     1501 aaggatataa atttatagcg tgtatgtcaa aaggaaattc tatggaaaga gcaagaatga
     1561 tgaaagcttt aggtgctgaa gtagttcttg tagaccaaat gcctaattca gttcatggac
     1621 aggtatctgg tgaagatttg gcacttgtag aaattgaggc tcaaaaaata gcaaaagaaa
     1681 gaaatgcatt tagagcagac caatttaatt tagaagcttg taatcatgca catgaaaagt
     1741 atacagcaga agaaatgtgg gaacaattgg atggaaatat tgatgtattt gtagattttc
     1801 ttggaagtgg tagtactttt gcaggatgtt caaaaacact taaaaaacat aattcttcta
     1861 tcaaatgtta tgttgttgaa cctgaaacag cagcaattta ttcaggtaaa gaaataactg
     1921 accaagggca taaaatacaa ggtggaggat attctatgga tttgccatta gtagacaaga
     1981 aacttattga tggttatatt caagttacag acgaggaggc aactgatgta gcaagaaaat
     2041 tagctaaatt agaaggtgta tttgcaggat tttcttcagg ggcaaatgta tgtgcagcaa
     2101 ttaagttatt aaagagcagt gaaaaaggaa aaaatatagt tttaactctt aatgatagtg
     2161 gtttaaaata tttaagtact gatttatatg aagaattgta ataatagcac aatagttatg
     2221 caatgttttt taaattaagt atatagattt aaattataaa aagtatctca atgtaatttt
     2281 tgaaagttat aaaggagata cttttttatt ttgtaaatat ggagtatcct acttataagt
     2341 attaagctat atagatattg atgattagct atgcaataaa aaag
//
LOCUS       DAEMIK010000272         2311 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEMIK010000272 DAEMIK010000000
VERSION     DAEMIK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.277"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            47..1051
                     /locus_tag="KPK87_003766"
     CDS             47..1051
                     /locus_tag="KPK87_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2539867.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGVKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKIIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1177..2289
                     /locus_tag="KPK87_003767"
     CDS             1177..2289
                     /locus_tag="KPK87_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2539868.1"
                     /translation="MKIKRILMVCFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
BASE COUNT          938 a          244 c          445 g          684 t
ORIGIN      
        1 taaataggat ataaaaatat cgtaaatgta tgaggagtga aatttaatgc gtttcaaaag
       61 aatattgata ttgatgttaa ctgtagcaat gatagctgga atgttggttg ggtgtgctaa
      121 taacccaagt aataatccta gctctagcaa agataataaa agtgatataa gcgataaaag
      181 taattcaaaa aaatctgtat caatgatact agatattgaa ggaacaaata atgaagctat
      241 gaataattca gctttattag cattaaacaa tgctcaaaag aaacttaata tagatacaaa
      301 caaagtggaa tcagatgatt cctcaacttt ttctaatagt atagatattt tatgtaatga
      361 caattacgat ttaattatag ctgtaggagc tagatttgca aaaccattgg agatggtagc
      421 caagaaatat ccaaagcaac aatttgcaat tatagactat gagtatgaca agcaaccttc
      481 taatataact tcaatttctt atgaagataa taaatctgga tatctagctg gattaattgc
      541 tggaaaaatg actgaaagcg ataaggttgg ttttatagga ggagtaaaga gctctagtag
      601 ggataaattt gaatcaggtt ttagagaggg tgttaaattt tctaatagta gtataaagga
      661 tatttcagtt gagtatgcag atgtttttaa agatagcaaa tcagtagaat ctatagctaa
      721 aaaaatgatg gataatggag tggatattat tttttctact actgaagatg attcaaaagc
      781 tgtaatagat gcagtaaggg caaaaaataa aaaagttata gcaacgaata aagaccaaca
      841 tgagttagct ccagaaaatg ttataagttc tattgtaaaa gattttgaga atcctactta
      901 taatctgata caaagctttg taaaaggaaa ttataaaggt ggaaaaatta tagaaaacac
      961 agttaagtct ggaagtactg ggcttgccca aaatagttct caaaatattc ctccagatgt
     1021 gttagaatat gttaataaga acaataaata atctactata atatccaaga atacttatga
     1081 ctttagttaa gaggtgaatt ggatgttata atagacatat atagaaagac atatgcagag
     1141 gtaggttaga cctatctaaa tcagggagtg aattttgtga aaataaaaag gatattgatg
     1201 gtttgctttg tagtagtttt tacaattttt atattagcta tatgtaattt tgattttggg
     1261 tttgataaag taaataagaa taattctaat atagatagtg ctaaatcgaa acagagtgag
     1321 tctaaaagtt tgccaacttc agcagaaaag gataaatcaa atagtaaaag taagaataaa
     1381 gaagatgaca cagaagatga ttcatatgta agaaaaacta ttggagtatt gttagatacc
     1441 gaaaacttaa ataacgaatc atataataac aatgtactat cagccttaaa aagggctgac
     1501 gaagattttg atgttaggat aaaacttgaa aaaccaaaga cttctaaaag ttttaaagag
     1561 agtatagaaa aactgcataa gtatgaacca gatttgataa tagcagttgg agccagattt
     1621 gcagagccaa ttaaagaagc tgctactaaa tatcccaaac agcaatttgc aatagttgac
     1681 tatacttata aagttcagcc agctaatgta atttctgtat catttcaaga taatgttact
     1741 ggatatttag caggacttat tgcaggtaaa atgactgata ctgggaaagt cggttttata
     1801 ggaggagttg agggttctag tagagataag tatgaatctg gatttagaaa ggggcttaaa
     1861 acttcaaata aagatgctga gttactagtt aagtattcaa atatatttga aaaccctgaa
     1921 tcagctagaa ctattgcaaa ggaaatgaga gattctgata gagatgttgt attttcagct
     1981 acagaagatg atagtaaaag agctattcag gtagcaagag aaaatggtgg taaagttata
     2041 gctattaata aagaccaata cgatatggct ccagataatg taataagttc aattataaaa
     2101 gatgtagaac aacctgtcta tgaactgata aagaatgttg caaaagatgg ttttaaggga
     2161 agtaaagtaa tggaatttaa aataaaagat ggagaacttg gcttgacttc aaagagcagt
     2221 agaaatatac caccagatgt tttggaatac ataaaaaaag agtataacaa agtgaaagat
     2281 acatattaat tattgatgta gaagattata a
//
LOCUS       DAEMIK010000273         2309 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEMIK010000273 DAEMIK010000000
VERSION     DAEMIK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.278"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(499..1083)
                     /locus_tag="KPK87_003768"
     CDS             complement(499..1083)
                     /locus_tag="KPK87_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2539869.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
     gene            complement(1405..>2309)
                     /locus_tag="KPK87_003769"
     CDS             complement(1405..>2309)
                     /locus_tag="KPK87_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG2539870.1"
                     /translation="IKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFSFY
                     TTAIAHRAGAEMAPENTLAAIEEASNAKADYAEIDVQETKDGELIVLHDSNFKRTTGV
                     DKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEMIKYSKGKVKLLIEIK
                     LNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAYGD
                     FSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITDN
                     PYLIESAIYWKKNGFISQVSDYLF"
BASE COUNT          804 a          370 c          218 g          917 t
ORIGIN      
        1 tttttatcca cttttatctc attaattaat tatattattt ttatgctttt tacaattttt
       61 cctatttaaa tatatattaa tataatcctc tttcataaaa taaatgttac tcttattaat
      121 aaaaaaaatt ataaccactt ttgattacta atcaaaagaa aatatctaag taaaataaaa
      181 attatattta ttaagcttta tgtattatag tattttattc ttacttaatc atcatactat
      241 agcaaaatac taacctaaaa gttagtattt tacttaaaaa taagtgctta ttcaaacatc
      301 aaatagaata tatactatta taaataaatc tataaaggaa tgacttttat aaaaaaatgg
      361 tggaaatgca agacagaagt gataaaaact acattccaca aaaatttaac aaatgaatat
      421 ggatgtgaaa gtattagaaa ataccctatg tataaagtaa gaaaggtgtt ttttgagatt
      481 ttgcaagaaa atttaacttt atttattttc tcgaataata cggcatggat ttccgtatgc
      541 aatcatatta cttggaatat cttttgtaac aatacttcct gcacctataa ctacattatc
      601 tccaatagta acacctggca tgataatagc acccccgcca atccaaacat tatctccaat
      661 agtaactgga gctgtttgag ttttacaaaa ttcaaatgaa ccatcttctt tggcattacc
      721 aaatctatca ccagcattag ttggatgaaa tgcagtatat atttgtacgt ttggtgcgat
      781 tagtgcatta tcaccaatga taattttatt atcatctaaa aacgtacaat tcatattgac
      841 ttcacaatta tttccaaaat aaatattatt tccataatct acataatacg gtgcagtaat
      901 ccataagttt ttacccttac caccaagtaa ttcatttaat attttttctt ttttttccaa
      961 atccgaggaa tctgtttggt tataatctct tactaggtct tttgctttat gccattgagt
     1021 taacaactca atatcaccac aatcgtaaag ttctcctgcc aacatttttt ctcgctctgt
     1081 catgttttta gtcctccaaa tttatattgt ttttcatata tattctagca taagatttat
     1141 taatttatat attttactta tataaaattt aataaataac aagatttatt aaatatacag
     1201 ttgacaatct atctctctgt catcttaaaa ctcttttaac tgtaataatc tcagagcaat
     1261 tttttcactt ttattccttg tttatagctg ataagcacaa tttttataag aataacctta
     1321 atttaacaac cttatttatg ttaaaaagag atattattga ttttaataat atctctttta
     1381 ctggataata caattataga atatttaaaa taaataatct gacacttgac taataaatcc
     1441 atttttcttc caataaattg cactctctat aaggtatgga ttatctgtaa taatactatc
     1501 tacatttagg tcaagcattt tctcaattaa actgtcacta ttaacagtcc aaacgaagat
     1561 ttttttgttc attttatgaa tcttttctac tactgtttta ttaacaaaag ttgattcaat
     1621 tccatatata tcaacataat ttaactttga aaaatcacca tatgcaattg ccataaaata
     1681 gcatgtcagt aaattctcat ccagtttttt tactttttgt aatatagttt tatccataga
     1741 tgcaactata cattgatttt caaaattatt ttctttaatt aactgaacaa ctttttttac
     1801 tacgtctccc ttttcatgtc cattaagttt tatttctatt aataatttca ccttaccttt
     1861 agaatatttt atcatttctt tgagtgttgg tatcttttcc ccaacaaact tttcatcata
     1921 ataacctttt ccataataat aaaagcttcc cgcatcatat gtctttactt cattataatt
     1981 tacatcccaa acatttttat ctactcctgt agttctctta aagtttgaat catgaagtac
     2041 aataagttct ccatctttag tttcttgaac atctatttct gcataatcag ctttagcatt
     2101 tgaagcttct tctattgctg ccaaagtatt ctctggagcc atttcagctc ctgctctatg
     2161 agcaattgct gttgtataaa atgaaaatga cgtattaaac ataccattta tatgtgaaga
     2221 aaatgcaact gactctatat aagaaatcaa taatattaat actaaattaa aaacaatttt
     2281 tgatatagat tttttctctt ttttaatat
//
LOCUS       DAEMIK010000274         2283 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEMIK010000274 DAEMIK010000000
VERSION     DAEMIK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.279"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..1216
                     /locus_tag="KPK87_003770"
     CDS             35..1216
                     /locus_tag="KPK87_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG2539871.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILDLEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            1200..1772
                     /gene="mobB"
                     /locus_tag="KPK87_003771"
     CDS             1200..1772
                     /gene="mobB"
                     /locus_tag="KPK87_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG2539872.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            1891..2166
                     /locus_tag="KPK87_003772"
     CDS             1891..2166
                     /locus_tag="KPK87_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2539873.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
BASE COUNT          970 a          184 c          413 g          716 t
ORIGIN      
        1 tcttaaataa aaaatatatt ggggagtatt taaaatgata gaattagaag atgccataaa
       61 aattattgaa aaaaatgttt taccaataaa cagggtcaag atagtaaatc ttatagatgc
      121 agtaaataag gtagttgcag aagatatata ttcaacaatt gatagtcctc catttgatag
      181 gtcaccctat gatggttatg catataatgc aaattctgaa aatagaaatt taaaggttat
      241 agggactttg tatgcaggag atgtatttaa tagggtatta aataaaaatg aagcagtgaa
      301 gataatgact ggtggaaaga taccacaggg tgcaaattgt gtaataaaaa aagaagatgt
      361 aacagttata ggtgatatga taactttaaa tataaaatta aaggaatacg ataattacat
      421 atttaagggt gaggatataa aaaaggggca attgattatt aataaaaatc agaatatagg
      481 atatgatggt attggagtat tagcaagtct tggaatatcc aaagtaactg tttatgatga
      541 tttaaaagtt ggaattttga atacaggtac agaactacaa gatataaatg agactttaga
      601 gaatggtaaa atatacgata gtaatagata taccttatat tcaagattat taaaacttgg
      661 aatacaagca gatacaatat gcaattgttc tgatgatata ttggatttag aaaaaaaggt
      721 aaagaatatg ttacagagtg tagatttttt aattaccaca ggtggtgttt ctgttggaga
      781 taaagattta attccagatg tattcaaaaa aataggggca acagaattat tttggaagat
      841 aaacattaaa cctggtggag cttgttatgt aggtacattg ggagaaaagc tattatttgg
      901 tctttctggt aatccacatg cagcaatagt ggtatttgat aatgtgatag ttcctgttat
      961 tgagtattta acatataaga agggaacaca tcatataaaa gctatattta aaggagaatt
     1021 taataaactt tcaaataaag atagatttgt aagtggaaaa ctatatacaa gtgaaggtaa
     1081 attgtatgta gagctaaatg ataagaaaga tgcaaaagct agactatctg caactctaag
     1141 aagtaattgt atgattaaga ttaaaaagaa tgagacaata aaagaaaatc aattagtgga
     1201 tgttgttatt agatagtttt atgaaaggac acaattttat gtataatata ttgtcagttg
     1261 tctcatattc aggagtaggt aaaactacgt tgattgaagg cataataaaa gaattaaata
     1321 aaaaagggta tcgtgttgga gttattaaac atacctgtca tgattttgat atggatgagg
     1381 aaggaaaaga tacttataaa catagaaaat ctggggcgag aaaagtctgt ataatatcag
     1441 ataaacgagt agcatatatt gaagaactaa aagagaagaa tccattatat aaaatgattc
     1501 aattatatga agatatggac ttgattatca tagaaggata taaaaattat agatttaaaa
     1561 gattggaagt aactagaaaa ggtaagtatg aggatatttt atctaataag tctgatttga
     1621 taggtattgt cagtgatata gaatataaat tgaatattga acagtttaat ttaaatgatt
     1681 ataaaaatat atcaaagtac atagagtctt gtataaaaaa tgaaatttta agtataagta
     1741 atgttgaaat aaaaaacata ttaaaagaat aattttaaga tgttcttctt atataaaatg
     1801 atataattat ttatataaat aaagatataa gaaatttatc aattttattt tgagttataa
     1861 aatattaaag aaattgtagg aggacaatat atgaaaaaaa tattagtagc ctgtggagca
     1921 ggaatcgcaa catcaacaat agtttgtgac agagtagaaa gattggttaa agaaaataat
     1981 atacaagcag aagttgagca atgtaaaatt gctgaatgtt caacaaaaca agaaggtgct
     2041 gatttaatcg tatctactac tatactacca acaacttatg atatacctac aataaaggca
     2101 acaggatata taacaggtat aaatacaaca gcattagata aaaagatatt aaatgcattg
     2161 aagtagtttt gtatttataa aaaaaattta gtattaaata tagagttgta tctttataaa
     2221 attttaaata tagagatata gctcttttta tgttgtttca agtatacaac tatatgatat
     2281 aaa
//
LOCUS       DAEMIK010000275         2264 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEMIK010000275 DAEMIK010000000
VERSION     DAEMIK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.280"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(163..1518)
                     /locus_tag="KPK87_003773"
     CDS             complement(163..1518)
                     /locus_tag="KPK87_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2539874.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          829 a          392 c          243 g          800 t
ORIGIN      
        1 tttttattta ataatcatac tctaacaaat attataatta tatatatcat aatattgtat
       61 ttagaagtaa aattttattt ttattattaa ttagttataa atcaatctaa tacaacataa
      121 ttacagtaga ttaacctata aaacataata attgcttact aattatttat catcctcaac
      181 aagtctactt aatgtcttat cagataagaa taataatatc gctgcaacaa atagtattac
      241 tggaattata gtatatactt taatcatatc aaatttaaga gtaaatgcat atatatatcc
      301 atatccttta ccagctataa aagtagcaaa tgtccatact cccataagaa cacctagaag
      361 cttcttaggt gcatacttac ttacaaaaga gttaccaagt ggtgagaata acatttcacc
      421 aagacttaaa agaataccaa acatgattat ccatagtata ctagctttag cagtttcagg
      481 agctccaact cctcttgaaa attctgcacc aacaagcatt aagaatgcaa gacctaagaa
      541 tattaatcca agacctgtct ttttaaataa actcatatct ccttgtggtc tactagcaag
      601 tttaaaccat aatgctccaa gaactggtcc taatactata catacaagtg aatttaatga
      661 gtcaaaccaa gcaagaggaa catcaaatcc tccaacattc atgtttacaa atgctgcacc
      721 ataatcataa actgcaagat aagttagata ccagaatacc cagaaaatta cagagaagaa
      781 tgatataagt ataatagccc aaattctctt cttatccatg cttgtaagag gagacttatc
      841 tttttcttca attttttcag caactacacc ttctttaaaa ggtctctttc ctgcattccc
      901 aaggaatctc catccatata tgaaccatat tgcacctatt acacaaagta cagctgctat
      961 aaagaaacat tgagaaaatc caagtacacc attttttgca aatgttttaa ggtaaagaat
     1021 accaactgct atagtaccaa taaaagaacc tatattaaca aaagaatact gtactgaaaa
     1081 tgctgtatca agttcttctt gactatcaaa aagctgtcca tttaccgcag atacgttacc
     1141 tttaaagaat gcagtaccaa ttgaaactaa gattatcatt gcatttacca tagaaactgt
     1201 agttgcaata gaaccaagat agtatcctac tcccatgata agcataccta taggtatagt
     1261 atatctagct cctatccatc tatctgatat atatccccca attataggtg caaggtatgt
     1321 gaatgcaaca aggttggctt gcattatagc agcttgtcct ttatctattc caagaccacc
     1381 atgaactata gctgttgtaa ggaatatata tattaagtac tttgaagcat aatatgctgc
     1441 tctttcaaat gagaaagttg ttgagcagat ataaaatccc ataggaaatt tacccttctt
     1501 ttttactgtt gagctcataa ttttaaatcc tttcttaatt gtatttttat tttttacgtt
     1561 ttgtccaaat tctttctttt atgaaagata taatattatt attttgtaaa acaatttgta
     1621 attattatta agataattgc gcatttattt gtagagtaca tattctcatg gaataaaatt
     1681 aatacacttc ttaatcataa atttaaatta tataaattca ctattttcag atatagttat
     1741 aaattaatat attctcatct aataccatga aaaaaccttc gccatatgca attcttattt
     1801 tttacgtgat agtattttac aaactaaaat cccccttttg tagcaactta tgtaattaca
     1861 ttttagtgct tttttaaagg agtctcgaat aaagcaacat atattttgtt caaataatgt
     1921 ttttatttat tttttaaatt tatatattac ttcacccaac caattctcaa cttaaataac
     1981 taaactacta tctattcaca gattaggctt atttcttaca cattagttta taataaacac
     2041 tgtgaatcta aacataaaaa ctgttatttt aaagtaatta cccatatatt cgctttgata
     2101 ctatttattg aggttatact ctttatgtat aatatattat ttataaaata cctcaaaata
     2161 tattattcat aaaatacctc aaactgaaaa ctttttcacg aatatttatt tttcacctcc
     2221 ccttttttat ttcaccatgt aaatcgtacc ataaatatgc actt
//
LOCUS       DAEMIK010000276         2230 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEMIK010000276 DAEMIK010000000
VERSION     DAEMIK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.281"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..1412
                     /locus_tag="KPK87_003774"
     CDS             99..1412
                     /locus_tag="KPK87_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG2539875.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1430..1966
                     /locus_tag="KPK87_003775"
     CDS             1430..1966
                     /locus_tag="KPK87_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG2539876.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT          881 a          211 c          410 g          728 t
ORIGIN      
        1 ttaaataatg tataatttat gctgattact gctaaaaaag cataataata ttgcattgta
       61 aggttttata aacttctaat tgaaagggga taattcaaat ggataagaga tatttagaat
      121 ccgatgtatt tgaagctttt caagaaagac tttcttatct ttttaatgaa tttgattata
      181 tctatgtatc ttttagcgga ggaaaagata gtggtttact gctaaattta gttttggatt
      241 ataagagaaa gaataatata agttccaaaa taggagtatt tcatcaagat tttgaggctc
      301 aatatagttt gactactgaa tatgttgaaa aagtatttga agataatatt gaagatattg
      361 aaccatattg ggtgtgttta ccaatggcta caagaactgc tgtaggaaat tatgaaatgt
      421 attggtatcc atgggatgat gagaaagaag agttatgggt aagagaaatg ccagtcaaag
      481 aatacataat aaatcaaaaa aataatcctt ttacttatta taaatataaa atgagacaag
      541 aaagtttggc aaaacaattt ggtagatgga ttagagatat acatgaaggt gcaaaagttg
      601 tttgcttatt aggaacacga gcagcagaat ctctacaacg ttattgttct attgtgaata
      661 aacagcatgg atataaagga aagtgctgga taactaagga atttaaaaat gtctggacgg
      721 catctcctat atatgattgg gaaatagaag atatatggtt agcacactat aaatttgact
      781 atgactataa taaaatttat gacttgttct ataaagctgg agtagctcca agaaaaatga
      841 gagtggcatc accttttaat gattcagcaa aagagagttt aaatttatac agaatattgg
      901 acccagatgt atgggtgaaa ttggttgcta gagtacaagg agttaatttt acttctattt
      961 atggcaaaac aaaagctatg ggatatcgta gtgttaaatt gccatatgga cacacatgga
     1021 aaagtttcac atattttttg cttgatacct tacctgaaaa tataaggaat agctatatta
     1081 aaaaattttc aacatctatt gagttttggc agaatacagg tggtggtttg tcagaaatag
     1141 ttatacgtga attgttgaga aaaggttata aaatagaatt aaatggagtt tcaaatcata
     1201 caaaagataa aaagagtaga gtcatatttt tggatgatat acctgatgat acggatgata
     1261 ttaaatttac caaagatata ccaagttgga aaagaatgtg ttattgtatt ttaaaaaatg
     1321 accatatgtg tagatttatg ggatttggtc ctacaaaaga acagagaatt agaacggaat
     1381 atattaagaa gaaatatgaa aaaattcatt agataggagt taagatgcta tggaatataa
     1441 aagtcctgta tatggaatca tcgcagtgcc aatcgaaaag atagaatcta acgactataa
     1501 tccaaatgca gtagccccac cagaaatgca gttgttgtat aaaagtataa aagaagatgg
     1561 gtatacaatg ccaattgtct gttattataa tgaggaaaag gataagtata ttattgtaga
     1621 tggttttcat agatatagag taatgcttga aaatgaggat atatatgata gagaaaatgg
     1681 acttcttcct gtttcagtaa ttgataaatc tcttgatgaa agaatggcaa gtactattcg
     1741 acacaatcgt gcaagaggtt ctcataatgt ggatttaatg agtaaaatta taactgaact
     1801 ttgtgaaatt ggaaaatctg atgaatggat atcaaagcac ctgggtatgg aagttgatga
     1861 aattcttaga ttgaagcaaa taactggatt agcttattta tttaaagata aagagtttgg
     1921 taatgcatgg attccctttg gctctgatat agaagaagaa aattaaatag atttattaaa
     1981 aaagaaaatt taaaatatat attaaaaaat aaagagtttt aatattatat gtaagtacag
     2041 aaaaaagagt aggaaaatta tcctattctt tttttatttt tataattata ctcttagaat
     2101 aaataaaatt atataactaa atgtatttta atgtaagctt attttaaaaa tagtattaaa
     2161 ttgtatattt atatcgtttt taataaaaaa taatcacatt ttatttaaaa atgaataata
     2221 tatagtaaat
//
LOCUS       DAEMIK010000277         2209 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEMIK010000277 DAEMIK010000000
VERSION     DAEMIK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.282"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..624
                     /locus_tag="KPK87_003776"
     CDS             <1..624
                     /locus_tag="KPK87_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2539877.1"
                     /translation="LYNNITHNMDVEFISYMWIIATPVSSIIMGNLNKSINELQNTNK
                     KLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTDFSLMIVKLPYYGNLQTI
                     FGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPNTSLEGSKVVKDRIKKRI
                     KELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEEELQYDV"
     gene            614..>2209
                     /locus_tag="KPK87_003777"
     CDS             614..>2209
                     /locus_tag="KPK87_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2539878.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASKITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGDGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLSIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKILIFIVSI"
BASE COUNT          952 a          202 c          340 g          715 t
ORIGIN      
        1 ttatataaca atattactca caatatggat gttgaattta tatcatatat gtggataata
       61 gcaacaccag tatcttcaat tataatgggt aatctgaaca aaagcataaa tgaattgcaa
      121 aatacaaata aaaaactatc tgaacagtat aaggaattag tgactattga tagtgaaact
      181 ggacttagaa atttaaagat tttttataat gatgtaaata tggagataag taaatctatt
      241 agacataata ctgatttttc tcttatgata gtaaaattac catactacgg aaacctacag
      301 actatatttg gtgaaaacaa aactaataaa atagtaaaat atattggttc aaatattata
      361 gaatgtacaa gaaatgaaga tataatttat agtttacaaa aagatatgat agggatacta
      421 atgccaaata catctttgga aggttcaaaa gttgttaaag acagaataaa gaaaaggata
      481 aaagaattaa acttagattt aaacaatagg ggaaaatatg taaatataga tgtcaaaatt
      541 gcatttttac aatataagag tagttttgga gatagtataa attttaagaa tatagttgaa
      601 gaggaacttc aatatgatgt ttaaaatatt taataaaagt ctattaaaat tagtagttgt
      661 attatcaata gtactgacaa tatcaaattt tagcttatct tcatatgcat caaaaattac
      721 ttccagcaca gataaggtat taatcatata tgattctaag aaagaaacag cttataatag
      781 agatatttta aatattatga ggactctatt gggaagattt agttcagata tagagctatt
      841 aaaattgagt aactatgatg gtgagataaa taaaaattat tacagtcata tatttatttt
      901 aggaatcaat gaaaactcat ataataatga taaaaacact aaaaacttga ttagttcctt
      961 aaattcatat aaaggaacta tatgttggtt aggagatggt atagaaaatc ttttggaaca
     1021 taaaaaatac aatttagact atgtaggaaa aacaaacaat atagttagtg taaattatag
     1081 aggtaaatca tataacttgg atgaacatta tgtttttaac attgtagaat caaaagatac
     1141 aagtaataaa gtaataggaa gtattaatga taccttaaat aagtatccat atataataaa
     1201 tgataaaaat cttttttatg tatcaaaatt agatttagat ggagttttat tttatatatt
     1261 ttgcgattca cttaatgata tttttaatat taagacgttt gataaaggac gtatatttgt
     1321 aagaatagaa gatgtacatg cttttagaga accaaaaaat cttgtagaga tagcggatta
     1381 tttaagtagt aagaatatac catttacgat tgcattaata ccagcatatg taaatcctaa
     1441 aaatcataaa gtaataactt tatcagaaag ccctgaaatt gtcaaagcaa taaaatatat
     1501 gcaagataaa ggtggaactg tgatattgca tggatatact catcaataca agaaagaaga
     1561 agtttctgga gaagggtatg aattttggga tggtaaaaaa gatgaaccct taaaagaaaa
     1621 tatgaagata tttgtcaaag atagagtttt gaatggtctt agagtatgta tagagaatgg
     1681 aatttatcct cttgcatttg aagcgccaca ttatgcaatg gaatcagagg gatataaaga
     1741 attaaaaaaa tatttttcta cttatatggg gcaacatcaa aataatgata aaaaatttag
     1801 cacaaataca tatccttata ttattaggga tacagaagag tttaatatat ttatacccga
     1861 aaatttagga tatatagacc cagaagataa gtttactttt caaaatataa aagaaaattt
     1921 agataaacta tccattgtaa gaggttttag tggagggttc ttctttcatt catatttaag
     1981 tatagagtat ctaaaaaaca caatagaata tttagaaaaa cagaatatag aatttatgaa
     2041 tttaagagat tttaataatt gggtaaaagt tgatgaaata caaataagaa ataatggaga
     2101 tgaaattata gtcaactatg ataaggactt agatgaacta actaaaagtg atactagatt
     2161 taaatccata tccaatatta gtaaaatttt aatattcata gtatctata
//
LOCUS       DAEMIK010000278         2207 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEMIK010000278 DAEMIK010000000
VERSION     DAEMIK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.283"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            305..1924
                     /locus_tag="KPK87_003778"
     CDS             305..1924
                     /locus_tag="KPK87_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG2539879.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT          814 a          269 c          323 g          801 t
ORIGIN      
        1 tatcattcta aaaagatgtt atttttttac atatttttta caattatagt taattctttg
       61 ttaaattgat tgtacataaa atactaaaaa tctaagttca agtatttaaa ttgctatata
      121 tcataaattt tctaaaatta acaacaactt aaaaatttaa cattctctta acgtatactt
      181 aacaaaacat taatattttt attgacaaaa tttgttattc tatattatgg aaacaataaa
      241 ttttagttaa taaagtttat aattcagata aaaaaattat taatttctta agggaggaaa
      301 tatcgtgaat atagtaatta gccttatcgg tggacttgga ctttttcttt acggaatgag
      361 tttaatggga gaaggtttac aaaaatcagc aggagacaaa ttaaaaaaaa ttattgaact
      421 cttaacaagt aatgttgtta tgggtgtact ggtgggtaca gtcgttacag gtattataca
      481 aagttcaagt gctactactg ttatggttgt aggatttgta aatgcaggta taatgaatct
      541 aagtcaagct attggtgtaa taatgggggc aaatataggt actacagtta ctgctcaatt
      601 agtttcattt aatcttgaag gaattgcacc aattgcttta ggtataggta ttttattcta
      661 tttatttaca tcaaatcaaa aaacaaagca tttatctgag atacttatag gtttcggtat
      721 attatttaca ggaatggagt ttatgaaaga tgcagtagct ccacttgctg aatataaagc
      781 attcactgat gcacttttat attttagtaa aaatcctgta ttaggtatat tggcaggatt
      841 tgccataact ggtattgttc aaagttcaag tgcatctatg ggtatgttaa tagcactagc
      901 ttctcaagga atacttcctc tatcatctgc tttacctatt ctttatggag ataatatagg
      961 tacttgtgtt acttcacttt tatcaagtgt tggtgcaagt agaaatgctc gacgtgcagc
     1021 tgtaatgcat ttaagtttta atgtaattgg tacaataata tttatgcttg ttttaaataa
     1081 acctatatca gccatagtta ctcattttga cccaactgat acagctagac aaatagcaaa
     1141 tgcacatact ttatttaatc ttacaaatgt tataatatta ttaccatttt ctaaatacat
     1201 agttaaatta gcaaatagac ttatacctat taaagaaact gaatcagaaa tagtaaataa
     1261 tactaagtac ttagatgaaa ggatgtttag cactccatct atagcacttg gtaatactgt
     1321 acaagaagtt gtaagaatgg gtcataaagc tacaaactct ttggaacatt caattgcagg
     1381 atttttaaat aagagcaatg aagatattaa taaagctttt gagtcagaaa aagttgtcaa
     1441 taagttacag aaagatatat tgaactattt gttaaaactt tctaaagaac ctcttagaga
     1501 tgatgaacgc tttagaacag atttgctttt taatactgta aatgacattg aaagagtctc
     1561 tgaccatgca gaaaatatag ctgaattggc catgagtgtt aaggaaatga acattagttt
     1621 ttctgatagt gcgataagag aaatatacga aatatacaat aagacaatta ctaattttaa
     1681 ggatgcctta atagttttag atgttaaaga ttttgagtta gcaaataaag ttttagaagt
     1741 tgaaaatgaa gtaaattatt tagaaaaaac ttttagaaat tcacatatga ttagactaaa
     1801 taatggaagt tgtacaattg atgctggtgt actttatctt gatttactta ctaatctaga
     1861 aagaatttct gaccactcaa caaatatagt aaaacaagta ctaaaactta aacaaaaaat
     1921 atagtgatta tttttatata taatcttaat caatgagaag taagaagcta tagataatgc
     1981 ttatatcatt tatggcttct tattttttat gtaatactca aatttttata gtattctctt
     2041 atattaaaca gtttctgcct agaatattcc atatatatat gacttctttc ataaaacttg
     2101 aactgcccat aaataaagtc acaagtatac ataactattt tttaatcaat tataaccaat
     2161 atttgtttta tttatcttca ttttaattct tcattcaata atgtaat
//
LOCUS       DAEMIK010000279         2188 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEMIK010000279 DAEMIK010000000
VERSION     DAEMIK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.284"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            207..2111
                     /locus_tag="KPK87_003779"
     CDS             207..2111
                     /locus_tag="KPK87_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2539880.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAESRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT          919 a          225 c          416 g          628 t
ORIGIN      
        1 ttagaataag tcttatttga aaattttagt gtattagagt aagtattatt ttaccagtaa
       61 aaaatattga gaactatatg tagatttgct taaaaaggaa tttacatata gttcttttcg
      121 tataataaat agtacaatct tatatggtaa agataaagtg tgatattaaa ttttattatc
      181 atccaaaatc taaggaggag tctcttatga aaataaaatt tattatagta gcatcaataa
      241 tatttttaat agtattactt gtggggtgtc aaagtactaa agacacagaa atcaatgtac
      301 tcgccacaag tgatttacat tcaattattc cagataattt aatttcctat gtagaagagg
      361 agagaaaata tgatgagaat ctacttatgg tagatgcagg agattttttt gacatgcaaa
      421 gtggagagat gaataagtgg tttactggtc aaaaattagt aagatttaaa gatgggatac
      481 cagagtacaa aaaaatagct gaatcacgtg aaggagaagt tccattggct aaaaaaatgg
      541 caaagctgaa gtatgatgca gttacatttg ggaatcatga atttgtatct aatgacaagc
      601 agagccttga taaattggtt tctgatttta aaaataacaa tataccatta gtatcagcaa
      661 acgtatatga acaatctgga gaaaactatg taaagccata tgtaatgaaa aatataaata
      721 ctgacaaggg aactgtaaag gttggtatac ttggtctgac tataaaagaa gtaggggaac
      781 gttcaaggtt taaaagtttt gaacaagata aaaaggctag agaacttggg gaacaacctc
      841 aatacaaagg gaaattatat gcaaatgatt tagtagaaga tgcaaataag tgggtaaagg
      901 ttatggaaaa aaaagaaaag ccagatataa tagttgccat tgtacattca ggtgaagagc
      961 caaaaaatcc taaaaatcca ggaaatagaa taaaagaact agcaacaact gtagagggaa
     1021 tagatgctat tgtagcaggt catacacatg aaaaaataga acagcataca tataaaaata
     1081 attctggtga agaagtaata gtaacacagc ctggagctca tggtaatagt atatcaaaga
     1141 ttaattttaa gttggataag aagaatgata agtggtttat aaaagacaaa tatagtgaat
     1201 taaaggtttt tggaaaggaa gtaaatataa ttacaacttc tggtctgaat ccaaaatttt
     1261 cagatgaaat ggcagttaat ttgtttaatg aaagagatag agatgttcaa cctatcttta
     1321 tagatactgg agatttttta aatactaata caaaagaaat gactaagtgg gctaaagagt
     1381 ggcaatacaa tgaaaataaa ggcatctata aaaaaataaa tgaatggcca attgtatatc
     1441 ttggttatga tgctgtagta cttggtagcc atgaatttac agaggataaa gagacttttg
     1501 aggaaaaaaa atctacattg gattatatta tagaaaactt tgaagaaatg aagataccag
     1561 tattatcagc taatatatat gatgaatctg gagaaaatta tgtaaaacct tatataattc
     1621 ataatgtaga aactagggaa ggaaatataa aggttggagt attaggtctt actactactg
     1681 ataaggatga agaagactta agtagctcaa gcttaagcaa agactcaaag acttcgaaaa
     1741 ttcctgaaga aacaggaaag ttatatacta acaatctagt aaaagatgca agcgaatggg
     1801 taaaagttat gcaaaaggaa agtcctgatg tgattgttgc agttgtacat tcagataata
     1861 atagtactct aaaaaatcca agtagtgaag tcaaaaaact tgcaatgtca gtagatggaa
     1921 tagatgctat tgttgtaggt catacgaaaa ataaggtaga agaacatatt tacaaaaata
     1981 agtctgggga tgaggttata gtaacacaat ctggagagga ttgttattca aaaataagtc
     2041 ttgaactaag aaaagaaaat ggcaagtgga atatcataaa caaatatagt aaagcaataa
     2101 aaatgaagtg aatttattga tatataaacc attattaata ctgattctac aatataatta
     2161 acactgattt ttataatata ataaatac
//
LOCUS       DAEMIK010000280         2187 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEMIK010000280 DAEMIK010000000
VERSION     DAEMIK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.285"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1141)
                     /locus_tag="KPK87_003780"
     CDS             complement(<1..1141)
                     /locus_tag="KPK87_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2539881.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDYYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNA"
     gene            complement(1272..1691)
                     /locus_tag="KPK87_003781"
     CDS             complement(1272..1691)
                     /locus_tag="KPK87_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG2539882.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            2004..2186
                     /locus_tag="KPK87_003782"
     CDS             2004..2186
                     /locus_tag="KPK87_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539883.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFIIKYGENIVD
                     SNLHSIMMLLCKWGLS"
BASE COUNT          778 a          302 c          188 g          919 t
ORIGIN      
        1 tagcattttt agatttttca aggagttttt tatttgtgtt taaggcattg tcataatatc
       61 caaagatata atatatcttt gcctggtgat aaattatttc taaatcatat attatatatt
      121 cttctatatt ttttatcata tttaatatgt ttttagctct atctaaatag catattgatt
      181 gttcaaaaga tttttcatta ctttttgcat aatacatggc taaacttata aataaatcaa
      241 acttctctac ttcttttaag ctatgatttt taattttttc tagatatact tcagattttg
      301 ctaaattatt tattgcgaca ggataatttc tagtctttat taatatttta gttatgttgt
      361 ttgatgctct atatttttga attatattta catttctata ctttttattg cttattactt
      421 cattgaaata ttccatggaa cattcataat tttcaaacga acattcacac tcacctatct
      481 ttatattaca tagtgaagat acttgcatat cctttagtaa atctgcatat tttcttgctt
      541 ttaaaaaatt taccttagca attagagcat catccaaaaa caaaaaatat attttaccaa
      601 taataaaaaa atatattttt ttacaattaa aatcgttaat ttcttttatc tttatataca
      661 acgtttctat ttctttaatt attacagaag attctataca cttacttgcc ttaatgtaaa
      721 taagttgtga tttaaaaata atttcttcaa ttagaaaatt attttcatca caaataagat
      781 taaatttatc catatataaa tttgccttat ttacatcttc attttttaaa cttaaaatag
      841 ctatttgtga ataaaatcgt gctaaaaatt tataatcttt gggtaagcat ttatcaatat
      901 atttacagga aatcaaaata tactctttca ttttttcact aaacccttga ataccatata
      961 ataaagctag caaaaaattt gattttaata acattaaact attttgttca gttttatagt
     1021 aatcaattaa attctttact acatcaattt tattttcaat aaaatcgtta ttagtcaata
     1081 atctttcatt caaggttagt ttaaacattg aatctaaaat tgtatcatct atctcttcca
     1141 aatatttact atatattgta actcacctcc gacttacatt atctcataaa tttagtaatt
     1201 ttccatacta tttttgaaaa aagttgatgt ctcaatttag aaatttctct taaattaaga
     1261 catactttac actaaactat acaattttct atctgaacta tataatcctg acaattttcg
     1321 caaattatca cacttacaat ataactatta tcaactttat ttgcccttga tagtttatta
     1381 aaagatagtc ctttataatc aacagatata ttgcttatta catattctcc atctttactg
     1441 aaaatataaa agtgatattc taaaaaagga aagaattttt tcttttcttt tactccttct
     1501 aaacataatg gacatgcttc ttgataatat tctacatcaa aatcaacttc ttctgcttca
     1561 ttaagtatat caattatttt tgaaatactt actccttgaa ctggattttc ttcatcattt
     1621 attaaagcac tacagttctc ctcttttaat aaatattcct tatcatcata tataaactta
     1681 tataaactca tattatatcc tccttataat tattaatctc caagttattc taccatttaa
     1741 ataaagttaa taaaagtaat tgtttataat ctttataatt aagttatgta tacatatatt
     1801 catatacact attttaatta atgattttaa aatccaagtt tgcactttga acatccagtt
     1861 tcccaaaatc tattgtcaaa actcacttta gttattaata taatgatata aatatataac
     1921 tgcatagatg ttacacactt aactatttaa tatgatacaa tttggtatct aaatttatat
     1981 attgtatata gggggagata tatatgtata aagataaaag tgatgaatgt atacacttaa
     2041 tgactgcata catcgatagt ataagtggat attattcatt tattgacaca caattagagg
     2101 attttattat aaaatatggt gaaaacattg ttgattcaaa tttacacagt ataatgatgt
     2161 tgctttgtaa atggggatta tcttagc
//
LOCUS       DAEMIK010000281         2051 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEMIK010000281 DAEMIK010000000
VERSION     DAEMIK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.286"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            52..666
                     /locus_tag="KPK87_003783"
     CDS             52..666
                     /locus_tag="KPK87_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2539884.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            684..1046
                     /locus_tag="KPK87_003784"
     CDS             684..1046
                     /locus_tag="KPK87_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539885.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1152..1649
                     /locus_tag="KPK87_003785"
                     /pseudo
     CDS             1152..1649
                     /locus_tag="KPK87_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
BASE COUNT          798 a          230 c          327 g          696 t
ORIGIN      
        1 acaaataaat acataaaatt taaactaatc tatttattgg aggagataaa attggaagaa
       61 gtaattgcaa aaattattac tatagaaaat ggattcaaag aaattgaaaa cattgctaaa
      121 aaaattgtaa aaaataatga ctcaaaaaag tgttattatc tatcaatcta cttatatagg
      181 tcagaatatt atcaagtaag agaattggct gtgtttatac ttggttttat atcagtaact
      241 atttctgaag ctttgttatt tttaaaagat aatgtgagta aagatgaaaa ctggagagta
      301 caagaaatac tagctaaatc atttgattat tattgcagtg aagtaggata tgaaaaggca
      361 cttcctgtta taaaagaatg gcttaattct gatagtccaa acataaaaag ggctgtgaca
      421 gaaggtcttc gaatttggac tagtagagac tattttaaat ctaatcctga cgttgctata
      481 tcactacttt ctagtttaaa agaagatgat agcgaatact tgagaaagtc tgtaggtaat
      541 gctcttagag atataagtaa aaaatatcca gatttagtaa aaaaagaatt agattcatgg
      601 gatatatcaa ataaaaaagt tgagtttgta tataagcttg cttcaaaatg tatcttggca
      661 aagtaaaatg gaggtgaact tatttgaaac ttcgtgatga gtatacttgc ccattagaac
      721 taacacatga tatgataaaa ggcaaatgga aatctattat attatggaga ttacgcatag
      781 gaccaacttc cttatcaaaa cttgaaaagg aaatcaatgg tattaatcaa aagatgttac
      841 ttgaacaact taaagaatta attgaatttg gtcttgtaga taaaaagaca tatgaaggtt
      901 acccacttaa agtcgaatac tcacttacac ctattcgagg gaaacaaata ttggatgctt
      961 tggctataat gcaagatata ggaattagct ttatgttgga taatggtatg gaagatattc
     1021 ttaaggaaaa aggtgtttta aaataaatag atacccacaa aaaagtaagt aatttaccat
     1081 tattttcaac tggtatataa ttaaattata gatatattct attaatttat tataatagga
     1141 ggtaaattat tatgaaagta actagtacag gtatagtaaa tggtgttata gaagataaat
     1201 acggtaaaag gggaactcag ttcaatgaag gtggtatgcc tacctattca ctacctctaa
     1261 agtttgaaga tgctccaaag aatacagtat cctttgctgt atttttagaa gataaagact
     1321 ctgttcctgt atgtgggttt gcatggatac attggttagc tgtaaatata acaaaagatg
     1381 agttaaatga aaatgaaagt gtaacagcta ctgattttgt acaagggact actagttagc
     1441 atggtaaact aggaggtctt gacagattag aggccagtgt atatggtgga atggctcctc
     1501 cagacagacc tcatgtctat gaaatacatg tatttgcact tgatactatc cttgaccttg
     1561 aaaaaggatt ctatatgaat gaatttttca aagctattga agggcatgtt ttagatacat
     1621 ttacactaaa aggaagttat tctaattaat taggttgact atagctaatc attaattaaa
     1681 tgctacattt aattgatgat tagcttaatt gttattagaa tattttctgc actttatctt
     1741 aatatatatt ttagttaact ggtattttaa tttctattag attattatca atttatataa
     1801 attgatttta gcttaaaact tacttatcta tttcagtttc tattagaatt attattagac
     1861 tcattaaaat aaaagcacta atataactaa taatatatgt taaattcatt aactctacat
     1921 aaagttttat aatctaaatg aaatacaata atctgcataa aattcaatag cttatataaa
     1981 atttaataat ttatataaac attatcaatc tatataaaat ttagtaattt atataaatat
     2041 tatcaaccta t
//
LOCUS       DAEMIK010000282         1946 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEMIK010000282 DAEMIK010000000
VERSION     DAEMIK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.287"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            372..1700
                     /locus_tag="KPK87_003786"
     CDS             372..1700
                     /locus_tag="KPK87_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2539886.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          687 a          218 c          339 g          702 t
ORIGIN      
        1 tatataaaag accttaaaga tagaaaacta cagaaagaag ccaaaatgtt tttgaaaaaa
       61 atgaaatttt attgcaaaaa aatcaaaatg aatttaactt aatagttata atctattttt
      121 agtgttacaa atatacaatg tatattatac aatataaaaa atacatttac ttagaagtat
      181 tattatattt ggatttttgg tttttataat ttttttatat ttaaacataa ctaaaaataa
      241 aagattacta aagctaagta gagtttattt tattttaata aataataaag ctatttttct
      301 atatattggc ataatatatg cttatttaac tatgaaatta aaattaaata ggattaatga
      361 ggaggaaaaa aatgggaagt aatgaactga aaaagactct tggcgtatca gcagcattat
      421 caactgttgt tggttccgtt ataggagcgg gcgtattttt taaaccacaa gcagtctata
      481 cattaactgg aggagctcca ggccttggta tattggcatg gttaatagct ggaatcataa
      541 ctataacagc tggtcttaca gctgcagagg tatcagttgc gataccaaaa actggtggaa
      601 tgatggtata tataaaagaa atttatggag aaaaattagg ttttttaact ggatggatgc
      661 aaattgtatt attttatcca gggatgatgg cagcattagg tgttatattt ggagaacaag
      721 catcagcact aattggtagt ccatcacttt tacttccaat agcaattgga attataataa
      781 tagttgcagg gcttaatatg ttaggttcaa aaactggagg agtcatacaa actgtttcta
      841 caatttgtaa gttaatacca cttattttaa taatggttgt tggatttatt aaaggtggtg
      901 gaaataatcc aatacttaca cctatggttg gtgaaggatt aagcctgggt agtgtactag
      961 gtcaagtttt aattgctata ttatttgctt ttgatggatg gatgaatgtt ggtactttag
     1021 ctggagaaat gaagaatcct ggaaaagacc ttccaaaagc tataataggt ggattgtcag
     1081 ttgttatggc tgtttatttt ataataaatt tagcgtatct ttgggtatta ccagctaatg
     1141 aattagcaaa ttatgcttct cctgcatcag cagttgctga agttatattt ggttcaatgg
     1201 gtggaaaaat aatatctgta ggtatattaa tttcagtgtt tggagcatta aatggatttt
     1261 tactaactgg ttcaagggtg gcatatactt tagctacaga taagacttta ccaaagtatg
     1321 gtatattttc taaattaaat tctgctcaag ttccagcaaa tgcaatagca ttagtatctg
     1381 ttattgcatc tatatatgct ttgtcaggtc aatttaattt attaactgac ttagctgtat
     1441 ttgctacttg gatattctat gtattaactt ttataggggt tatgaaactt agaaagactc
     1501 atccaaatat accaagagag tataaagtcc cattatatcc tattgttcct ataatagcta
     1561 tagcaagtgg tatatttgtt gtagttaatc aattgtgttt tgcaggtatg aaaactacta
     1621 tgatatctat aggaggtctt gtaataacag ctataggctt accagtgtat gcttatatga
     1681 ctagaggagt aaaaagataa accaagtagt ttacaaatgc ttcataatta aaataaaaga
     1741 gactgtctca aatagagata agtctctttt ttattgttaa aatggtaaag atatgtttaa
     1801 ttttacaaaa aaagagcgtt ccatgaacta aaaagtttat tttaaggtat tcattttatt
     1861 tttagttttt tgattataaa attaaacatc taaaaaatta tttattttaa aatactttat
     1921 tttaaagtgt tttttatcaa actaag
//
LOCUS       DAEMIK010000283         1941 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEMIK010000283 DAEMIK010000000
VERSION     DAEMIK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.288"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..391
                     /locus_tag="KPK87_003787"
     CDS             <1..391
                     /locus_tag="KPK87_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539887.1"
                     /translation="KGFNIDKIDFVYSVKPIDVFLKTFPTLLWYTTFPLMILTLTKNR
                     FTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYMPNNIDVRFNELKNYFFI
                     NRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(487..1290)
                     /locus_tag="KPK87_003788"
     CDS             complement(487..1290)
                     /locus_tag="KPK87_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG2539888.1"
                     /translation="MEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDSVLYLLELGVN
                     PDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGYASDIRMQKTF
                     QHFLDTQYTDGGWRCNKFNFGRGPETEYSNPLPTLNILNAFRFSNYLNKESKLDKAVD
                     FLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCAKKDNRFLEAL
                     KILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLEK"
     gene            complement(1375..>1941)
                     /locus_tag="KPK87_003789"
     CDS             complement(1375..>1941)
                     /locus_tag="KPK87_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG2539889.1"
                     /translation="KELDPHTISNLSNIPFNNDLNRFTLNFFDRQYIIDYPSGEVWNS
                     NGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIPGGNVYYPNFLNRTLYRLSEVF
                     TSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMIFILWFGDDEFPPNSNILFDSN
                     IVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT          686 a          265 c          251 g          739 t
ORIGIN      
        1 gaaaggtttt aacatagaca aaatagattt tgtttatagt gtaaagccaa tagatgtgtt
       61 tttaaagacg tttcctacat tattatggta tacaacattt ccactaatga tattaacatt
      121 aacaaaaaat agatttacaa caatagcaat attaattaca tatacatttg ctgatatatt
      181 ttttatttta catatgtacc catttgttag tatgattaat gtaaatggat tttatattca
      241 aaagatgttt tatatgccta acaatataga tgttagattt aatgaattaa agaattattt
      301 ctttataaat agaggttctc tagtagttat ttctataata atagtttttt atatagcaag
      361 aaaatcaacg gttttaaaaa agagtaaata gtttaatata ttttttatat tattatattt
      421 atatagaata gtgactttaa aaaatttaaa gccactattt tttaatatgt tttatctata
      481 taactattat ttttctaggt tttttaatat ttcgtaatag tgtgttgtgg ctatttcact
      541 tgactcgcct tttttacaaa aagataattt ggatagtttt ggaactacac gttcaacgac
      601 aattttatta cctatcattt tagattcaag tatcttcaat gcttctaaaa atctattatc
      661 cttttttgca caattataaa atgataaaac gtagacataa aggaataggt tataattacg
      721 gaaaggatat tcaacttgca taaataatgt gccaattcca tagtgacaag ggccgattgg
      781 ttttttaata gtccaatggt caagtagaaa atctacagct ttgtcaagtt ttgattcttt
      841 atttagataa ttactaaaac ggaatgcatt taatatatta agagttggta gtggatttga
      901 gtattcagtt tctggacctc gaccaaaatt aaatttattg caacgccacc caccatcagt
      961 atactgtgta tctaaaaaat gttgaaacgt tttttgcatt ctaatgtcag atgcatatcc
     1021 catatgacag agtacgttag cagcatttgc agtatgacaa gggtaaatac ctccatttgg
     1081 atatagtttg aaacgtccat cttctcgcca tgtgctaaat atcaagtcag cacattcttt
     1141 aagtaaagta tcatcagggt tcacacctaa ctctaataga taaagtacac tatcaagagt
     1201 agagaaagga gaacctttta ttaaacgctt atcaggtgta gtccaatagt ctgcaccatt
     1261 atcatacctg tgtgataata ttatttcaat atctgataaa gattgatttt ttactccatt
     1321 tcatattata ttctattaaa tctatcaaaa accttattat aatattattt atatttataa
     1381 ttgatttatt ttatttaaaa ttgcatttat agctgtatca ggaacaactg ctaaatcttc
     1441 tgcattgaag taataaacta tattagaatc aaataatata tttgaatttg gaggaaattc
     1501 atcatctcca aaccaaagta taaatatcat agatgtatta cccatatatg taaacctata
     1561 agctaaatct cccatgttta ctttttcagc tccaattttc tccataacta acttatattt
     1621 gtctatttga gatgtaaaaa cttctgaaag cctatataga gtcctgttta aaaaatttgg
     1681 ataataaaca ttgcctcctg gtatttcttt aaaagttaca tatttattag ttctaggaat
     1741 acctttagca ttgattaaat atctaactat caagatttta agctcaaagt tattgcaaag
     1801 gctaccatta ctattccaaa cttctccact tggatagtct ataatatatt gtctatcaaa
     1861 aaaattgagt gtgaatctat ttaaatcatt attgaaaggt atattactta aattactaat
     1921 tgtatgaggg tctaattctt t
//
LOCUS       DAEMIK010000284         1924 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEMIK010000284 DAEMIK010000000
VERSION     DAEMIK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.289"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            74..448
                     /locus_tag="KPK87_003790"
     CDS             74..448
                     /locus_tag="KPK87_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2539890.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            445..1308
                     /locus_tag="KPK87_003791"
     CDS             445..1308
                     /locus_tag="KPK87_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2539891.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREYYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1308..>1924
                     /locus_tag="KPK87_003792"
     CDS             1308..>1924
                     /locus_tag="KPK87_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2539892.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSK"
BASE COUNT          732 a          204 c          295 g          693 t
ORIGIN      
        1 aatcagttga catattatat atatatttat ataatgtgta tatactatat atacaattta
       61 aggaggtgtc ttattgaata tagttattag taattcgagt gggaagccaa tatatgaaca
      121 gattactaca caaattaaaa gtatgattat aagtggagag ttaccagaag gctcagctct
      181 tccaagtatg agactattgg caaaagaact tagaataagt gtaattacta caaaacgagc
      241 atatagcgat ttagaacgag acggatttat tgagacagtt actggtaaag gcagttttgt
      301 atctagtaaa aacatggatt ttgtcaaaga agaacaattt cgtttaattg aagaatacct
      361 acaaaaagca gttgatacag caaaaagtag tgatattaca tatgacgaat taaaagatat
      421 tttattttta ttatataaag gagaatgata agtatggatg caataactat aaaaaattta
      481 aataaaactt ataaagattt tagcctacaa gatatatctt tttcagtacc aaaagggagt
      541 gtaatgggat ttgtaggtga aaatggtgca ggaaaaacaa ctacattaaa agcaatttta
      601 aatttaatat cttatgatag tggaaatata gaaatctttg gtcttgatag taaaaaaaat
      661 gaaaaagaaa ttaaagagca aattggggtt gtatttgagg gaagcaactt tcatgaaaat
      721 ttaaacactg accatgtttc aaaaatcatg agtaaaatct ataaaaattg gaatgacacc
      781 ttatttaaag actacttaaa aaaattgaga gtaccagaca ataaattaat taaggagttt
      841 tcaaagggga ataagatgaa acttagtatt gctgtagcct tatctcataa accaaaatta
      901 ttaatactag atgaggcaac tagttcactt gaccctatcg ttcgtgaaga aatactagat
      961 atatttttag attttataca agatgaagaa cactctatta tcttatcttc acatattaca
     1021 agtgatttag ataagatagc agactatatt acatttatac ataaaggaaa aattgtattt
     1081 agtgaaaata aggatgaatt aattgatact atgggagttt taaaatgtaa acctagtgat
     1141 tttgataacc tttcaagaga gtattattca tattatcgta aaagccaatt tggatatgaa
     1201 gtacttctta aagataaaca tagatttatc tcaagacatc caaactgtat tgttgataat
     1261 acgtctatag aagaaattat gttattttat gtgagaggtg ataaataatg aaaggtctaa
     1321 ttttaaaaga tttacttaat ttgaaaggaa atataaagtt tatactttta ttcatcatta
     1381 tgtttggctt tatgtcttct ttaggagatg gaaatgttaa taactttata ggtgttatta
     1441 tagttttatg tactacaatg attgtttcaa cattttcata tgatgatttg aataaatggg
     1501 atagttatgt acttacaatg cctattaatc gcaatgatat tgtattaagt aaatatttaa
     1561 ctatgttaat atttagcttt ataggtgttc ttgtttcact tattgttagt gtaactattg
     1621 gttattttaa aaatacactt atattaaatg aaacactact tattaatgca ctaatcttat
     1681 caatttcagt atgttttggt agccttattc taccacttat ttataaattt ggtacagaaa
     1741 gagctcgttt attaatgatt ttatgctttt tagttccaac actagcttta ttagttttta
     1801 aaagtatttt agaaaatatt agttcaccta tttctataga aattatactt aatacactag
     1861 tatattctct tccatttgtt gctattttac tatttgtgat ttcatatttt atatcatcaa
     1921 aaat
//
LOCUS       DAEMIK010000285         1921 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEMIK010000285 DAEMIK010000000
VERSION     DAEMIK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.290"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..711
                     /locus_tag="KPK87_003793"
     CDS             136..711
                     /locus_tag="KPK87_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539893.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            809..1807
                     /locus_tag="KPK87_003794"
     CDS             809..1807
                     /locus_tag="KPK87_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG2539894.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
BASE COUNT          798 a          167 c          328 g          628 t
ORIGIN      
        1 tagaggattt ataagtgttt aaggtgaatt aaatataaga caacttctta aagcaaatgg
       61 gtgtggtatt taaatctaaa tgtagacata taatattaaa tatgctaata tatcaaaata
      121 ataagggggg aatttatgaa gcttaaagaa gcattgatta catgtgttgt atgtagtgta
      181 tgtttaactc cattattggt aaatggagaa acaaaaatag atgcagatca ttgtaaagaa
      241 tctatagtaa atatagatac tatagtaaag tcaggtaaaa gctttaattc caagtataat
      301 caaggtatgg aagattataa aaatagtgat gagtataagt tgaaagtaca aaaggagaaa
      361 ctacaaaatc agttaggtat taaaataaag aagttagtac ctataacttt tgaagttagt
      421 tattacacaa gtcttaatca agaaaatgga tatggagcga taactgctac aggagatgat
      481 ttagaagatg gttttgtagc aaacaatcac ttaagttttg gaactaagat tattgtagat
      541 ggaaaattaa aaactgttat ggataggggg tctagtaaat attttagtaa ttctaatgca
      601 atagatgtat ttgtgcccaa atggagtaat gaaagtgaat cagagtacta tgatagagtc
      661 aataatatgg gaagacatta caaaaaaggc tacattattg ttgaagaatg atgttctttg
      721 aagtaatatg tattgattgt agtatttaat atatatataa taaatataaa actattagta
      781 caatgagata tattactagg agggcaatat ggaacaatcg tttataaatg gaaaagtaaa
      841 cttattaata gaaaattaca aagcattaaa tgaagttaaa ggttcttggc aaatgggatt
      901 gatacaacat agttgtgcac ttgcatttac ccttaagaac aagagaataa gtcctagatt
      961 agtggaagaa cgaatagagc ttattaaaaa aaatactgga ttattctcta attttagagg
     1021 atataatatg ttttatatgg caacactact ttcgtttgaa tcaaatccag aaagtagttt
     1081 taagatgatt ttagatatat acaaagaact taaaagtgaa aagttttggg gcgatacata
     1141 tttaccactt acagcatcaa tagtttatga aaatagagag aaaatggatt atcttacatg
     1201 tatatcaaaa atgaaaataa tatatgatta catgaggaaa aaacatccat ttctaacttc
     1261 tagtgatgac tattgtaata tagcacttat tgcaattcat tcaaaaaatt tagatgaaga
     1321 tttagaatac atagaaaaat gttatgaatt tctaaatgaa aatggttttt ataaaggaaa
     1381 tgatttacaa gcattatccc aaattctttt atttagtgat gatagaacta tgcttaaatg
     1441 taaaaagact attgaactta aaaaagcatt taaagaaaat gattgtaaaa tgaattatta
     1501 tggatatcca ataataggag caatttcatt attagattat agagaagatg aaataataga
     1561 agagataaaa aatgtttcaa ataaacttaa agaagaaaaa ggctttggaa attggtcttt
     1621 aggaaaaagt aatagagtaa tgattagttc tgctattgta gcttcaattt atgcagattt
     1681 gatacgaaat gagaataata taggctctat aactaataat atatttttaa atattgttgt
     1741 tgctattcaa attgcatgtg taatggcagc aacaagtgca gctatagcat cttcttcatc
     1801 aaattaataa gatttattga agttggatat attaaattta ataatacaat ttatatgttt
     1861 aaaaagaaga tagtattaca ttttaattaa ctgtgatatt atataatatg aaacatataa
     1921 t
//
LOCUS       DAEMIK010000286         1895 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEMIK010000286 DAEMIK010000000
VERSION     DAEMIK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.291"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            85..201
                     /gene="rrf"
                     /locus_tag="KPK87_003795"
     rRNA            85..201
                     /gene="rrf"
                     /locus_tag="KPK87_003795"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            208..282
                     /locus_tag="KPK87_003796"
     tRNA            208..282
                     /locus_tag="KPK87_003796"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Asn,seq:gtt)
     gene            287..361
                     /locus_tag="KPK87_003797"
     tRNA            287..361
                     /locus_tag="KPK87_003797"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Glu,seq:ttc)
     gene            367..442
                     /locus_tag="KPK87_003798"
     tRNA            367..442
                     /locus_tag="KPK87_003798"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Val,seq:tac)
     gene            448..524
                     /locus_tag="KPK87_003799"
     tRNA            448..524
                     /locus_tag="KPK87_003799"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:482..484,aa:Asp,seq:gtc)
     gene            535..609
                     /locus_tag="KPK87_003800"
     tRNA            535..609
                     /locus_tag="KPK87_003800"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:567..569,aa:Thr,seq:tgt)
     gene            624..708
                     /locus_tag="KPK87_003801"
     tRNA            624..708
                     /locus_tag="KPK87_003801"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:658..660,aa:Tyr,seq:gta)
     gene            718..791
                     /locus_tag="KPK87_003802"
     tRNA            718..791
                     /locus_tag="KPK87_003802"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:750..752,aa:Gly,seq:tcc)
     gene            802..878
                     /locus_tag="KPK87_003803"
     tRNA            802..878
                     /locus_tag="KPK87_003803"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:836..838,aa:Arg,seq:tct)
     gene            888..963
                     /locus_tag="KPK87_003804"
     tRNA            888..963
                     /locus_tag="KPK87_003804"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Gln,seq:ttg)
     gene            975..1050
                     /locus_tag="KPK87_003805"
     tRNA            975..1050
                     /locus_tag="KPK87_003805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1008..1010,aa:Lys,seq:ttt)
     gene            1053..1141
                     /locus_tag="KPK87_003806"
     tRNA            1053..1141
                     /locus_tag="KPK87_003806"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1087..1089,aa:Ser,seq:tga)
     gene            1159..1249
                     /locus_tag="KPK87_003807"
     tRNA            1159..1249
                     /locus_tag="KPK87_003807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ser,seq:gct)
     gene            1258..1334
                     /locus_tag="KPK87_003808"
     tRNA            1258..1334
                     /locus_tag="KPK87_003808"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1292..1294,aa:Pro,seq:tgg)
     gene            1420..1495
                     /locus_tag="KPK87_003809"
     tRNA            1420..1495
                     /locus_tag="KPK87_003809"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1453..1455,aa:Trp,seq:cca)
     gene            1556..1632
                     /locus_tag="KPK87_003810"
     tRNA            1556..1632
                     /locus_tag="KPK87_003810"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Pro,seq:tgg)
     gene            1639..1715
                     /locus_tag="KPK87_003811"
     tRNA            1639..1715
                     /locus_tag="KPK87_003811"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1673..1675,aa:Ile,seq:gat)
     gene            1719..1794
                     /locus_tag="KPK87_003812"
     tRNA            1719..1794
                     /locus_tag="KPK87_003812"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1752..1754,aa:Phe,seq:gaa)
     gene            1802..1878
                     /locus_tag="KPK87_003813"
     tRNA            1802..1878
                     /locus_tag="KPK87_003813"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1836..1838,aa:Met,seq:cat)
BASE COUNT          478 a          440 c          516 g          461 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaaatgtggt tataatagcg gagaagatac acctgtttcc
      121 attccgaaca cagaagttaa gttctctagc gctgatggta cttggtggga aactacctgg
      181 gagagtagga catagccacg tgaatgttcc aaggtagctc aacggtggag caaccggctg
      241 ttaaccggta ggctgtgggt tcgagcccca cccttggagc cattatggct cgttggtcaa
      301 gaggttaaga caccgccctt tcacggcggt aacaggggtt caattcccct acgagtcacc
      361 atttgtggga tcatagctca gctgggagag cacctgcctt acaagcaggg ggtcacaggt
      421 tcgagccctg ttggtcccac catttgcggc ctggtagttc agttggttag aatgccagcc
      481 tgtcacgctg gaggtcgagg gttcgagccc cttccaggtc gccaaaataa aaatgctggt
      541 gtggctcaac ggtagagcag ctgacttgta atcagcaggt tgtaggttcg attcctatca
      601 ccagctccat gtaaaactaa tacggaggat ttcccgagcg gccaaagggg gcagactgta
      661 aatctgttgt catcgacttc ggtggttcga atccaccatc ctccaccaat taaatatgcg
      721 ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt cgatcccctt
      781 cacccgctcc aaagaaagcg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      841 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatatattgg ggattagcca
      901 agtcggtaag gcacatgact ttgactcatg tatgcgtagg ttcgagtcct gcatccccag
      961 ccaaataaaa atatggtcta ttagctcagt cggtagagca cctgactttt aatcagggtg
     1021 tcccgcgttc gagtcgcgga tagatcacca atggagaggt gtccgagtgg tttaaggagc
     1081 tggtcttgaa aaccagtgac ttcgaaaggg gccgtgggtt cgaatcccac cctctccgcc
     1141 aaaaaaatga ctgaatttgg agaagtactc aagtggctca agaggatccc ctgctaaggg
     1201 attaggctgg gtaaccggtg cgagggttcg aatccctcct tctccgccat tcaaaaacga
     1261 ggtgtagcgc agtttggtag cgcacatggt ttgggaccat ggggccgggg gttcgagtcc
     1321 cttcacctcg accaacttaa gcttttagtt cagttggtta gagtgcccaa ctcatggcta
     1381 gtaattctag ggtttgagtc catgaaggtt attcttcata ggggtgtagc tcagttggta
     1441 gagcgtcggt ctccaaaacc gtgcgccggg ggttcgagtc cctccacccc tgccaaataa
     1501 aaaggacttg aaaaaagttg gattttagag tagtataaga tagtaaataa gatttcgagg
     1561 tgtagcgcag tttggtagcg cacatggttt gggaccatgg ggccgggggt tcgagtccct
     1621 tcacctcgac caagaaaagg gcctatagct caggtggtta gagcgcacgc ctgataagcg
     1681 tgaggtcgct ggttcgagtc cagctaggcc caccatttgc ccagatagct cagtcggtag
     1741 agcaggggac tgaaaatccc cgtgtcggtg gttcgattcc gcctctgggc accaggaata
     1801 cggcggtata gcttagttgg ctagagcgtt cggttcatac ccgaaaggtc ataggttcga
     1861 ctcctattac cgctaccatc taactatatc aataa
//
LOCUS       DAEMIK010000287         1861 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEMIK010000287 DAEMIK010000000
VERSION     DAEMIK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.292"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1829
                     /locus_tag="KPK87_003814"
     CDS             <1..1829
                     /locus_tag="KPK87_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2539895.1"
                     /translation="IINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVNDSDET
                     TLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGHRYID
                     ISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTILNLSK
                     DGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDKVVVF
                     NKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFKDIKT
                     NSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVNCAAV
                     PENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLNEIGDMPLELQTKLLRVLQ
                     EKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPLRERK
                     EDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLEKDLP
                     PNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQITEG
                     KIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT          848 a          192 c          267 g          554 t
ORIGIN      
        1 aaattataaa taatatacta gataagaaaa aagtgattgt ggtaagaaga acttttaggg
       61 aagataaaat ttataatcta ctatctttac cacagggtac aaatgttcta atcgtaaatg
      121 attctgatga aactactcta gaaactataa gtttatttta taaaattgga gtgactaata
      181 ttagaccaat tccatatatg aatgataata actataaaaa tataaaaata gctataactc
      241 caggagttcc agaaaaagta cctagcttta tctcagatat atttgattta ggtcacagat
      301 atatagatat ctctacattt atagaaataa taaatttatt acaaattgat tcaaaggaaa
      361 tacaatctaa tctagttaaa tactcagaag aaataataag tttagatact ggtataaaag
      421 ataaatataa ggaattattt ttaaaaatag aagaattaga tacaatcttg aatttatcaa
      481 aagatggtat attatttact tcaaaagatg gtgaaataaa tacatataat agtaaagtaa
      541 aagatatttt agacataaat gaggatatat atggaaaata cattgaggat atatttgtag
      601 atagtttaaa agttttattg agtgagaaag agatactaga taaagttgtc gtattcaata
      661 agaaatatat aaatgtaaat aaaaaaaaca tatacaatag agatgaaaaa atgggcacat
      721 attatagttt acaagaaata acatacataa aaaaattaga acaaaatctc actaaaaaat
      781 taagagaaaa aggacaaata gcaaaatata catttaagga tataaaaaca aatagtccta
      841 aaatgtttga atgtatagat ttagctaaga aggtttctaa atctgattta agcatactta
      901 tcagaggtga aagtggaact ggtaaagaac tcatagctca gtctatacat aacaactcaa
      961 atagaaaaaa tcaacccttt atagcagtaa attgtgcagc cgttcctgaa aatttattag
     1021 aaagccagct attcggatat gacaagggaa cttttactgg aggtctaaaa gatggaaaac
     1081 aaggattatt tgaacttgct aataatggaa caatattctt aaatgaaata ggcgatatgc
     1141 ctttagaatt acaaacaaaa ctacttagag ttcttcaaga aaagcagata atgcctgttg
     1201 gatctcacaa tgtaattaat atagatgtta gaatcatatc tgctacaaat aagaatttag
     1261 aacaaatgat tgataatagc caatttagag aggatttata ttatcgatta aatacaattc
     1321 caataaatat acctcctcta agagaaagaa aagaagacat attaattatt atggaagatt
     1381 taattaataa gaaattagta atcacaccag aagcaaaaaa acttatacaa aactatatgt
     1441 ggaaaggtaa tataagagaa ctccaaaatg ttacatccta tttaaacatt atgtgtgaag
     1501 atattgtatt agagaaagat ttacctccaa acttaaggtc ttcagataac aaaaatacat
     1561 cattaaaact aaaatatagt aagaatgata tattgaatat attagagata ctaattttaa
     1621 ataaggaatc agatgttggt attggtagag gattaatttt aaaagcacta ctcgataaga
     1681 accttcaaat tacagaagga aaaattaaaa aaatatttga atatctaaaa aaagaagaac
     1741 ttataatttg tagttcagga agatatggaa gtaaaataac tcaaaaagga gaagattttt
     1801 acaataaatt aaaatataaa ggtctataaa attataattt atcaaacaaa gaaatctatt
     1861 c
//
LOCUS       DAEMIK010000288         1844 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEMIK010000288 DAEMIK010000000
VERSION     DAEMIK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.293"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            94..378
                     /locus_tag="KPK87_003815"
     CDS             94..378
                     /locus_tag="KPK87_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539896.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(535..708)
                     /locus_tag="KPK87_003816"
     CDS             complement(535..708)
                     /locus_tag="KPK87_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBG2539897.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1149..1364)
                     /locus_tag="KPK87_003817"
     CDS             complement(1149..1364)
                     /locus_tag="KPK87_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539898.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1375..1842)
                     /locus_tag="KPK87_003818"
     CDS             complement(1375..1842)
                     /locus_tag="KPK87_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG2539899.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          750 a          198 c          233 g          663 t
ORIGIN      
        1 ataaagtaat ttgaagaata tggaaaaact tgattagtac attattaatt agggtataaa
       61 atggtgtaaa tagaatgata aggggtggat gaaatgcttg tatataataa aagtttttat
      121 cctaatgaca tatttccaag attagatttt tcaaaaataa aaaaacagtt aaaattgata
      181 gataatgacc tgtcagattt tggaagcata tgtataatag aaaaagaaca ttatacaata
      241 agtgtaaaca gtataggtga aataaatgtg tattatgatt tagagtacga aaataaggtg
      301 tatagaatag tttatgagat tgaaaagtta tttaaatctc aagttggaag gtttagcata
      361 tctacataca gaaattgata attaaaaaag tagaaattaa aaaacttaat actaaagata
      421 tagataaata tatgaggtaa cagctagaga gagttgttac cctttttaaa tactaagtat
      481 atattattct tattaatttt ttgtaaattc actaaaagac ctttttatag aatattattt
      541 tcttttttta ttaaataggt aaatcagtaa aaatgcaatt ccagttccaa ttattattcc
      601 aatgatattt tctataatta atttttttaa tataaaagta aataataaaa atgctataca
      661 taaacctaaa atatatcctt taaaataggt tgaaagtttt cttttcatat aatcaccaac
      721 ttgtatcaga tttagtttac ttaattagag tataacagtg ttgtaacttc aaagagtatc
      781 taaaagatta caaatgttag ataaaatcat caataatgta atttataaca cgtagctata
      841 aactatgcaa taatataaat ggaggtataa gtaaatgaaa aagttccatg ttttgctaac
      901 agtttaaata aatattaggt agaaaagaat gtatatagga atctgtggta aaattatcaa
      961 tttaaaagta ttatttagtg tgatacagtt cttataggta atggataaca aatagataaa
     1021 ggaacagttt ctttcttaga acatattttg ttatgacttt aaacaaagtt aatattaatg
     1081 tcaataatat agtaaaagaa taaaaatcat atagtataat aaaaaagagt gtttctaaac
     1141 actctttttt aatcattttc atcttctaca tattcaagta tatcacctgg ctgacagtct
     1201 aaagctttgc aaatttcgtt tagagtggta aatctaatag cttttgcttt tccagttttt
     1261 aaaattgata aatttgcatt tgtaatatct attttttctg ccaaatcttt aagtgatatt
     1321 ttcctttttg ccatcattac atctaggtta actactattg ccataacatc acctctatat
     1381 cgttaaatca ttatcttctt ttatttttat agctttatca aatgtttctg caattacaaa
     1441 gcacattagt gcaaatataa aagagataat cataagtggt gagataccgc cactatcagt
     1501 tccaaaaatt cttatattat tagtatttaa attataacca ttgatacatt caattatagt
     1561 gtttatcaat aaacaaaatc ccattacctt aaaccttttt acattttcaa gtacgaatgg
     1621 ggaatactta gtactattaa ttataaatat caagaataag atgactataa agtagtcaga
     1681 aaaatatagt attcttgcaa ttaaagcgat tactttgtca gaactaaaat catataaaaa
     1741 cataggtatc atacataaga atattatacc agcaactcct aaaagtgata atagaaaata
     1801 tagaaatact gataatatat tactaaaaaa ttttgtaatc ataa
//
LOCUS       DAEMIK010000289         1776 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEMIK010000289 DAEMIK010000000
VERSION     DAEMIK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.294"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            502..1611
                     /locus_tag="KPK87_003819"
     CDS             502..1611
                     /locus_tag="KPK87_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2539900.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIAKGKREEKF
                     NIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
BASE COUNT          738 a          168 c          296 g          574 t
ORIGIN      
        1 ggtatttctt ttggttcttg tttagcaatg attatttctt atactgcatg gaaatctatt
       61 ccatgggcta tttttcatgg cttaatgagt tggatatatg tactttatta ttgggttaag
      121 tatgcataga ttaaattaat aaataagtat gtaatattgt aaagtttaag aaacaaaatg
      181 taagggagtg tctcgaagtg aacttttagt ttataaggca ctcttttttg tattaaataa
      241 aatatatttt cattacttaa acaatgaaaa agatgagtat ctctatttta atacataccc
      301 ttatattcat tcatttatca acaatagttt taaaaagaaa gctatctaaa atctattgta
      361 atctaagtgc ttctctttaa agtcttaact ttagtaatga tatataagtt tataaggttc
      421 ttatttcaat aatgcaaatt ttacttagaa gttcagactt tggtatgaag aaatgggtaa
      481 taataaaaaa gaggtgttga tatgaaagaa aaattagaag aaaaatctaa gattattaaa
      541 acatcaaaag agtataaacg aactataatt gaagaaaaag acagagaatg gaccaaaaaa
      601 ttaaatgaca tacttagtag aggtgatttt tctaatgaaa aattagataa aatcaaggat
      661 ttaattccaa aagaaatatt gactagtgaa aatgttggag aagttgtaac tgaagatggg
      721 tatgctaagc ctgagtatga tgaggtattt aaatcattgt ttactttaaa taatgattac
      781 gatttacttg caaattttat aaatgatatc ctcaaagatg ctccatatgc taataggaat
      841 atcaaacagt tcactcaaat aaaaagaata attaaggttg aaaccgaccc aacaattaat
      901 tacattggtg agaaaagacc tagacttgat attttggctg aagatgaaga acgtaaccat
      961 ataaatattg agatgcaaag agctcttgaa gaggattatt tggaaagagc tgaatattat
     1021 cttagtcgtg tacatggtag aaagttagaa gaaggtaaag aatataaaga aattggaaaa
     1081 actgttggaa tacatatact aaatcatgta aaatataatc acatagaaga ttatgtaaat
     1141 tgcctgagat taacaatgga cgggcatcca gatatttttt ctagtaaaac tgcattatat
     1201 tttatagaac ttccaaaaat tcgtaaatcc agttgtatag caaacagagt tctaatatgg
     1261 ggtaaactta ttgataatcc ttctcatata gatattagaa tactatctaa aacggattat
     1321 gttattaaaa gagcacttga tagattaaaa gaattaggta gtaatgagga ctatttatta
     1381 aatttaaaga gaggtgcgta tattatgaat aaaagtagaa attttaaaga agaaattttt
     1441 caagaaggta ttgaaaaagg tatcgaaaaa ggtattgcaa aagggaaaag agaagagaaa
     1501 tttaatatca tcataaaatt aaaaaataaa ggatacaatt tgagtgaaat atgcgatatt
     1561 atagatgatt tgaataaatc agaggttgaa aaagtctata atcaaaacta atactatgat
     1621 tgagatggca ctagataaat acaaagcttc ctcttttaaa tttaaattaa tataaaactt
     1681 tgctatttaa atatggtaga tataaagaga tatataaata ataggtacaa agaggtgtat
     1741 aaataaagga gataattcaa aataaaagtt tgaacc
//
LOCUS       DAEMIK010000290         1766 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEMIK010000290 DAEMIK010000000
VERSION     DAEMIK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.295"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(63..1439)
                     /locus_tag="KPK87_003820"
     CDS             complement(63..1439)
                     /locus_tag="KPK87_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG2539901.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     regulatory      complement(1533..1635)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT          650 a          322 c          202 g          592 t
ORIGIN      
        1 acaattgata ataaattaca gccctttatt ttatattaaa ttaaaatttt aaataatacc
       61 tattatagta gccaaagttt tattataaat aatattgcca gaacaatcat tactacagaa
      121 acatgttttt tgtcttctgc ctttttagct ccaaatatat tataaaatat atttacgaaa
      181 acataagtta atatacctaa agttaaacca tcacctatac tatatgttaa tgccatacaa
      241 gcaatagtaa caaaagctgg aacaccttca gttatattat caaactctat atccttaact
      301 gtaccaagca ttaaatatcc aacatatatt aaagctggtg ctgtagcaca tgatggaatt
      361 gctataaata caggagagaa aaacattgca gctaagaata aaattccaac tgttattgct
      421 gtccatccag ttcttccacc tgcgataact cctgttgaac tctcaacata agttgtaact
      481 gtagatactc caagtcctgc accaactgta gttgcaaccg catctgtaag taaggctcta
      541 cctacattag gaacattccc tttttcatct aacatgtttg ctttagaaca aacaccaact
      601 aatgtaccta ctgtatcaaa aaaatcaaca aataaaaatg tacaaactac tactaaaaaa
      661 ccacctatat ttgttggatg tagtacatat cctaaatcta ttttacctgc tattggtgct
      721 aaactttcga atttaaataa tccaccaggt aaatatatac ctaatttttg tgcatactct
      781 ggatttataa aagcaaatgc ccaagcaagt aatgaactca ctactattcc aaaaagtata
      841 gaacctttaa ctcttttttt atctaataca gcaataatta ttaatcctac acaagttatt
      901 attactgctg gtgtaaatct acccattttg ataagagtgg cttggtctcc aataacaatt
      961 ccactgttaa ctagacctat aagtgcaata aatattccta taccacctgt aacagctaat
     1021 ttcatatttt ttggtatcgc atttacaaca gcctcacgaa ctccacttac tgataatact
     1081 ataaaaataa taccttctac aaatactgct gtaagagcta cttcccaagg aacacccatt
     1141 tgtaaaacta ctgtatatgc aaaaaatgca ttaagaccca tacctgatgc taaagcaaaa
     1201 ggaaggttag caactacacc cattaaaata catccaaatg ctgctgctaa acaagtacct
     1261 gtaacaagtg cacctgcagg catgcctgtc tcagataaaa tatttggatt tacagctatt
     1321 atataagcca tagttaaaaa agtagttact cctgcaatta tttccttctt catgtcgaca
     1381 tttttattag tcaaaattgg aaaaagtttc tgcatagtac tactgtttgt tgtttgcata
     1441 acaacacctc ctattaaatt ttacaaaaaa acgacacagt agttgataag ctgagccgta
     1501 tccttcataa ttcagttaat taaatattta attaactaaa taatagtata catagtcaga
     1561 atatttacgg tattccggta gaaacttatg acccatattg tcaaaattat atgttataca
     1621 gtacaaaaca tgaatttttc tttacgaata ttattttaat ttatataatt aattttgtca
     1681 atatatacgt atatattgta ttgattttgt attaatattc gattatatta aatattgatt
     1741 tattaaattc aaattataat tatatt
//
LOCUS       DAEMIK010000291         1766 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEMIK010000291 DAEMIK010000000
VERSION     DAEMIK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.296"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            342..1682
                     /locus_tag="KPK87_003821"
     CDS             342..1682
                     /locus_tag="KPK87_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2539902.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          699 a          131 c          278 g          658 t
ORIGIN      
        1 gttactgctt aaagaagcag agattttatc aaggtatatc atggaaaaaa atattttatt
       61 gtaatattta taagatttta ttatggaaat aatgtaaaaa ctttataaat aatatatttt
      121 atacctagtt atagataaat ttatatagtt attattaaaa aaagcaataa aaagtttaat
      181 tcaatttagc aaatttagtg atataattaa aaaagaaaag aaatatggac ttatttggaa
      241 taattttgac aatctattta ctacacgtgt agagtcgtat ataattaaat tatacataaa
      301 atataaaaaa ataatttagt aatacgttag gagaaagtat tatgataaag tcaagcttat
      361 tttggaatat tttcattgcg atatccttta tgatagaatg gtttctttgt aagaaaatgc
      421 tagattatac aagtgagaaa aggatttcta acaaaaaaat aaacttttgt atggtaggta
      481 ttattgtttt tgctgacata atatattttt ttgatgtacc tccaaatata agagtaatta
      541 tatctatgat aattactatt gcattttata aaatctcata tgatgtgggg tttataaagt
      601 ctgtattaat atccttaatt tattggatgt tactaatcgc cacagatgct ttgagtatat
      661 ctttaacagt ttggcttaat tctataaatg acatgagtag tatggggacg ggagatatat
      721 acagaattga gtctataata ttgggaaaga tacttttgat tgtgtctttg cttttatata
      781 gagccttaaa gataaaactt aatatagaaa taaataaaag gacattattt tatatagtta
      841 ttcctatagt cgctaatata gcatcttttt ttgtaatttt tgaatacata tttaatccag
      901 ggtctggtag cttaatacat aatttccaat tcgttatgat ttctgttgta ttgcttatat
      961 caagtatcac tttagtttta attgctaaaa aattacagca atataatact ttagttgtag
     1021 aaaacaatat gattaaaaat actttgaagt atgaaaatga gtattatgaa gatatagaaa
     1081 agaaatattt aaaaactaga ttactaagtc atgatatgaa aaatcacata ttgtgtataa
     1141 atgctatgat taaaaaaggt attgatgtta cttcttattt aaataatttg caggtagaaa
     1201 ttcagagtaa tgacttgtta tttaatactg gtaattttat attagatgct atattaagtg
     1261 aaaagaaaga ggtttgtgat aagaatgata tagaactaaa aataggaata aattttagtt
     1321 gttgtgaagc tgttgaatta gtggatatat gtcatatatt ttcaaatatt ttagacaatg
     1381 caattgaggc atgtaataaa attgatagtg gaaatagagt tatttgtgtt aaaggtgatg
     1441 ttatgcaaaa tttttattta ataagagttg aaaatactaa gatgaataag attattacta
     1501 aaaatggaga tattttgaca gacaaaaaag gttctcttca tggacttgga ataaaaagtg
     1561 taaaaaactc tgtaaataag tatggtggta atgttgtaat tgaatatgat gataaaaaat
     1621 ttgtagttaa aattgctatt ccacttagaa atgtgaagaa agcagttaat catgtgattt
     1681 aaatgtatat aaatttgatg tgtagaaatt taatatataa gaattaatgt ataaaagtga
     1741 tgcatagaag tttaatatat aagaat
//
LOCUS       DAEMIK010000292         1664 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEMIK010000292 DAEMIK010000000
VERSION     DAEMIK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.297"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1193..1582
                     /locus_tag="KPK87_003822"
     CDS             1193..1582
                     /locus_tag="KPK87_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2539903.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          741 a           96 c          291 g          536 t
ORIGIN      
        1 atgggaaatt tataatataa ttaatgaaaa aatttaaact ttgtaatata taaataaaaa
       61 aactctaaaa tagaaggata taattatata gaatgtttta attaaacaag tcaaaaacta
      121 taatcttaaa attaacatta acattattct taagaagtaa gaaatttaag aagtaaaaag
      181 tttagtaata attcatagca agtaagaaat ttagaaagta ggaagtttag tgataagtgt
      241 tagtaagcaa gaaatttagt aagtgagaag tttagtgata agtgttagta agcaagaaat
      301 ttagaaagta ggaagtctag taataaatta tagcaagtaa gaaatttagt aattgagaag
      361 tttagtaata agtcgtagta agtaagaaat ttagaaagta ggaagtttag tgataagtgc
      421 tagtaagcaa gaaatttaga aagtaggaag tttagtaata atttatagca agtaagaact
      481 ttagggagta ggaagtttag taataattta tagtaagtaa gaaatttaga aagtaggaaa
      541 tttagtaata atttatagta agtaagaaat ttagaaagta ggaaatttag taataattta
      601 tagtaagtaa gaaatttaga aagtaggaag tttagtaata atttatagta agtaagaaat
      661 ttagaaagta ggaagtttag taataaatta tagcaagtaa gaaatttaga aagtaggaag
      721 tttagtagta atttatagta agtaagaaat ttagtaagtg agaagtttag ttataattta
      781 tagtaagtaa gaaatttagt aagtgagaag ttcagtaata agttgtagta aataaaaagt
      841 ttaggaagta ggaagttcag taataagttg tagtaaataa aaaatttagg aagtaggatt
      901 tactgaaata caaatattta aaatcaaaga gtttaaaaga aaggagagct actggacaag
      961 tatccaaaat agatagctga gtacaaaata tttcataaaa ttaagcaata taatacaaga
     1021 gaattttgac attatttata tatatttacc gtgtacaaat agctaaattt aggattaaga
     1081 ttgaaaaata tcttataagt ctgagcttat ataaatccgt ataagatgta tttaatcgaa
     1141 aaattttatt atggatattg cttgtctata ttctgagtag caaggtttta tcatgactat
     1201 aaaaaagttg ccacaatcag aattaaagat aatgaaattt atatggaaat cagattccaa
     1261 agtaacatct agagatattg ttttaggaat ggagcaaaaa tatcaatgga aacaaacgac
     1321 aactttgact cttttatcta gattagtagt aaaaagattt ctaaattctc aaaagataga
     1381 taaatatacg cattatgaag ttttaattaa agaaaaggaa tacataggag ttgagacaag
     1441 agattttttt agaaatattc atgattcttc tataaaaagt ttattgttgt cattacatga
     1501 aaatattaat ttgagcaaag atgatatttt acttattgaa gagtggataa aaaatctaaa
     1561 agaagaggag aaagacgtat aagcaaacgt atttcttata gcatcaatat aaagtgtatg
     1621 taagtgtatt tatgtaatat attagtctac ttaagtaata tgac
//
LOCUS       DAEMIK010000293         1585 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEMIK010000293 DAEMIK010000000
VERSION     DAEMIK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.298"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..1282
                     /locus_tag="KPK87_003823"
     CDS             83..1282
                     /locus_tag="KPK87_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539904.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKAKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1291..>1585
                     /locus_tag="KPK87_003824"
     CDS             1291..>1585
                     /locus_tag="KPK87_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539905.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIW"
BASE COUNT          697 a          122 c          243 g          523 t
ORIGIN      
        1 gttttttaat tataatgttt attctaaatg gaatatctta tttgctaatt aaaaagaaag
       61 tatctcttta aggaggggtg gtatgaaatt aaaatctaaa atatctatac ttattatatt
      121 tttgacttta gcaatatatt ttagtatagg tttattaaat aatgaagata atttaaagat
      181 aaaaactata aaaggtgact ctaatgaatt agaccatata aacataaatt ttggatatgc
      241 tttgaatgga tatataaatt catattatac aataaatgga agcaacaaag ccaaaaagac
      301 tgagttaata aaactacaaa aggatagtaa gaacacaaat aatttaattg atgtatctag
      361 tggttcagat ttaattgctc atgttggatt tgaaatgaat gaggagtcaa aagatataaa
      421 ctttgtgata gatgagaaaa aagaagattt agaaaaaggt gtatttccat cagaaggtag
      481 tgataaagaa attgaatatg aaaaattaga tatgaataat tttacaagga caagatataa
      541 agtagataaa agtacagatg attattatgc aaagatacta tcatcaaaaa gatataaaaa
      601 atacttatat gtatctgtac tttatgctac aagtgattca tcagaaaata acaaagaata
      661 ttcaagtact atagatataa taaaaattaa tccagaaaat aaaaaaatca agactataaa
      721 aagtataaat ttaagtaatt atttttccga aaaataccaa aacatagaga acataggtgt
      781 aagttatgct agcaaatatg aggataagat gtatatttta gtaaatgtac taaatacaaa
      841 cataggtgat aatagtaatt ctaaatttca tttacttgag tataatcttg aagaaaataa
      901 ggaaagcata atggaattaa aaactgatag caaaatggct ttagcaaaag caaagttaga
      961 agctaataaa ttatatatgg taatgtcgaa tgattatgat aatagttcaa ttacagagtt
     1021 aaatttactt acctataata tattagataa aagcttagat gaagataaat atgttttaga
     1081 taatagagaa aaaagaggaa ttagaagtat tgaaattgat tcaaataaaa tcgttgcata
     1141 tacatcaagg ctaaactcta tattatcttg ggataataaa tttatgctat atataataaa
     1201 taaaaattct aaggaaattg tatatgaagg agaattacaa tttaacagtt caaatagtat
     1261 gttagaattt gaaataaagt aggtgagttg atgaaagatt taataagatt attcgaattt
     1321 gaattaaaaa gaaatataaa gaattatata tttataataa tttgttgttg ttcttttgta
     1381 attttaaaca tagtaaaaaa tttaaatgac tataattaca taatagaaaa tgcagttaaa
     1441 agtgaacagt taacaaaaat cggaaatacg atagaaacgg taaatgttac aggtttctca
     1501 agctttagaa atataatggg ttctacagaa tcgtggttta tgtttggtat aattgcatgt
     1561 atttgctatg ctttttttat ttggt
//
LOCUS       DAEMIK010000294         1562 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEMIK010000294 DAEMIK010000000
VERSION     DAEMIK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.299"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="KPK87_003825"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="KPK87_003825"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="KPK87_003826"
     tRNA            179..253
                     /locus_tag="KPK87_003826"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="KPK87_003827"
     tRNA            259..344
                     /locus_tag="KPK87_003827"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="KPK87_003828"
     tRNA            360..435
                     /locus_tag="KPK87_003828"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="KPK87_003829"
     tRNA            443..517
                     /locus_tag="KPK87_003829"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="KPK87_003830"
     tRNA            532..605
                     /locus_tag="KPK87_003830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="KPK87_003831"
     tRNA            611..686
                     /locus_tag="KPK87_003831"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="KPK87_003832"
     tRNA            692..768
                     /locus_tag="KPK87_003832"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="KPK87_003833"
     tRNA            779..853
                     /locus_tag="KPK87_003833"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="KPK87_003834"
     tRNA            863..936
                     /locus_tag="KPK87_003834"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
BASE COUNT          494 a          276 c          330 g          462 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacact gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataataa atataactaa ttagtaagag ttatatacat tatattgtat
     1201 actttatggt atttatatat tgaaataact tatgaagtta tttataaaga gattcatgag
     1261 catatttttg tctaaacaat aagtataaga aattataaaa aattagagac tatagtgaat
     1321 ttacaattta atgtaatatt tataagttat actaaataaa ttattatact gtgaaatgga
     1381 tatataactt taggtattgt gtattatttt cactgtagga gattatatct agttatacat
     1441 aataagtttt aatgatagtt tatagtaaga tattaaaata aatttatcta atctagatat
     1501 gaaataagtt attactttaa tataaaaatg aacctaatgg aataatattg atgataagta
     1561 at
//
LOCUS       DAEMIK010000295         1535 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEMIK010000295 DAEMIK010000000
VERSION     DAEMIK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.300"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    366..629
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            780..1331
                     /locus_tag="KPK87_003835"
     CDS             780..1331
                     /locus_tag="KPK87_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG2539906.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          618 a          155 c          233 g          529 t
ORIGIN      
        1 gtaatattct gtttcaaagg aggcatataa atacaggaat gagtattaaa tgatggttaa
       61 ttaagtaaat ttattgataa aggtacttaa ttaactttga agaatttaat tcctcatgaa
      121 tcaaaatata aaattacatt aacagttatt cacaaaatta atataagaag aattagagcc
      181 aaagagcaga ctattagacc attaaagtaa ttattcatag aattttgtat atgaaaagaa
      241 atgtatagta taatttactt tttttataaa aaatagatta caaaatatat tttttataaa
      301 aaggtattga cattttttag aaaaaagaat atcattattt taataacaaa attcaataca
      361 ataaattaaa tcctatgaca aggaagagta gttaaattta ggttcagtat agagagctga
      421 ggatggtgga atcttagcaa gaaaaaattt gatgaatggc ccttagagga ataaattgaa
      481 atcatagttt tgagagtaga ctttatcgtc agtcgcacgt tatagcgaat aaggtatgta
      541 agtacctgat aatgagatgt atgaaaattg aattttatat taatttttat ataatttagg
      601 tggcaacgcg gataatctcc gtcctattag tttaggacgg agttttttat tttactgaaa
      661 atttaaaaga tagaaggtgg tgagaaagat gttatgaatt tcaaaagcta aagatgatga
      721 tatagtaggt tggcaaaaaa ataaattgaa aattaaaaaa ataggaggaa gtacaaaaaa
      781 tgaaaacaaa tacaaaaaaa ttaactttaa atgcaatact tttagcgatg ggattattaa
      841 tacatcaaat cacaccagct ataggattac caatgaagcc agatgtacca ctagcaatgt
      901 tatttgtaat attggtttta aatagagatg attataaaac ttgtttgata gcgggtatag
      961 taacaggaat atttacagca cttacatcaa gttttccggg aggtcaaatt cctaatgtaa
     1021 tagataaaac tcttacagca aatatagtat ttttattaat gaatattagt tataatatgc
     1081 cttttataaa aaaattagct aagaaaacac aagatactgt agtagttgca ataataatgc
     1141 caataggaac tcttgtaagt ggaactatct ttttattagc agctcaagct atagttggtc
     1201 ttccaggggc ttcatttaca gctttattct tagcagtagt attaccagct gttttaataa
     1261 atctagttgc aggaatattt ttattcaaga ttgttagtct atcaataaga agagttagtt
     1321 atcaagctta aacacgaaat aatattttac tttataaagc attataataa ctaaataaca
     1381 taagaataaa gtagattata actattttat tcttatgttg tttaaagatt tggtattata
     1441 tatttatttt taataaaata tttatatagt accaattaca cttaatttaa cagataaatt
     1501 atcataccta tataatattc ttatatgtat ctcat
//
LOCUS       DAEMIK010000296         1517 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEMIK010000296 DAEMIK010000000
VERSION     DAEMIK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.301"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1517
                     /locus_tag="KPK87_003836"
     CDS             <1..>1517
                     /locus_tag="KPK87_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBG2539907.1"
                     /translation="KDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDT
                     INVLPTITEGIPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIA
                     ATVASIVGIGAEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYG
                     PLKTEDDKILVPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPS
                     ISSHIPSLSIYSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRL
                     LDSIRDLYPGKFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIR
                     NKLSYSFDGAGGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKL
                     IKDVLSKIDINKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSY
                     SLLLSGDKNYLISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKS"
BASE COUNT          595 a          187 c          228 g          507 t
ORIGIN      
        1 taaagatgta ctgaatgatt taagtacctc agttaaggtt caactttatg ctcaactatt
       61 tagtacaggt ttaaatacta tatatgactc tatccaatta gtaaatttaa tatcaaatgc
      121 agtaaatgat actataaatg tactacctac aataacagag gggataccta ttgtatctac
      181 tatattagac ggaataaact taggtgcagc aattaaggaa ttactagacg aacatgaccc
      241 attactaaaa aaagaattag aagctaaggt gggtgtttta gcaataaata tgtcattatc
      301 tatagctgca actgtagctt caattgttgg aataggtgct gaagttacta ttttcttatt
      361 acctatagct ggtatatctg caggaatacc ttcattagtt aataatgaat taatattgca
      421 tgataaggca acttcagtgg taaactattt taatcatttg tctgaatcta aaaaatatgg
      481 ccctcttaaa acagaagatg ataaaatttt agttcctatt gatgatttag taatatcaga
      541 aatagatttt aataataatt cgataaaact aggaacatgt aatatattag caatggaggg
      601 gggatcagga cacacagtga ctggtaatat agatcacttt ttctcatctc catctataag
      661 ttctcatatt ccttcattat caatttattc tgcaataggt atagaaacag aaaatctaga
      721 tttttcaaaa aaaataatga tgttacctaa tgctccttca agagtgtttt ggtgggaaac
      781 tggagcagtt ccaggtttaa gatcattgga aaatgacgga actagattac ttgattcaat
      841 aagagattta tacccaggta aattttactg gagattctat gcttttttcg attatgcaat
      901 aactacatta aaaccagttt atgaagacac taatattaaa attaaactag ataaagatac
      961 tagaaacttc ataatgccaa ctataactac taacgaaatt agaaacaaat tatcttattc
     1021 atttgatgga gcaggaggaa cttactcttt attattatct tcatatccaa tatcaacgaa
     1081 tataaattta tctaaagatg atttatggat atttaatatt gataatgaag taagagaaat
     1141 atctatagaa aatggtacta ttaaaaaagg aaagttaata aaagatgttt taagtaaaat
     1201 tgatataaat aaaaataaac ttattatagg caatcaaaca atagattttt caggcgatat
     1261 agataataaa gatagatata tattcttgac ttgtgagtta gatgataaaa ttagtttaat
     1321 aatagaaata aatcttgttg caaaatctta tagtttgtta ttgtctgggg ataaaaatta
     1381 tttgatatcc aatttatcta atactattga gaaaatcaat actttaggcc tagatagtaa
     1441 aaatatagcg tacaattaca ctgatgaatc taataataaa tattttggag ctatatctaa
     1501 aacaagtcaa aaaagca
//
LOCUS       DAEMIK010000297         1485 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEMIK010000297 DAEMIK010000000
VERSION     DAEMIK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.302"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            34..525
                     /locus_tag="KPK87_003837"
     CDS             34..525
                     /locus_tag="KPK87_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539908.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYTGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSF
                     KIE"
     gene            553..>1485
                     /locus_tag="KPK87_003838"
     CDS             553..>1485
                     /locus_tag="KPK87_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539909.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSL"
BASE COUNT          664 a          123 c          205 g          493 t
ORIGIN      
        1 ttaatatata gatacaggga gattgatgcg attatgtatg aaaataataa ggtaatatac
       61 aaccttgaaa gatgtgaaaa actagaaaag actctttatt tatctcaatt taatacatat
      121 aaaaatttta aattagcttc taaaaaaact gtaataaatt cattagagac atatgtttac
      181 aatatgaata ttaacaatat aagtcttgtt gatactatag aatgtatatc attagaagat
      241 gaacaactta caggaaagaa gttgatttta gttggtgata tagatattga tgcaatatta
      301 gattatacag gcaataaaag aaatagaaat tctagtaaaa aaaatacatt taaattaaaa
      361 atacccttta gtacatttat acaaatgcct agaaagatag aaaataaaga taaaattaat
      421 ttaaagtatc ttatacaaga tattacttca tctattttag atgataattt atttattagt
      481 gttactgcaa ttataagtta tgaaaatagt ttcaagatag agtaatcttt aaattataga
      541 aaggtggatg caatgagaat aacgaaaact acttttaatg atgtattttc aaatgatgca
      601 aatggaagta ttataaaagt agaaaaatat gttgataaaa aatgtgcatc tgtatttgag
      661 attttgacct acactattat tgttacaaat acaagtaggt acaaaactgg aaatatattt
      721 ttcaaagatt atatttctaa atatatagag tttataaata atactgtaaa agttaatggc
      781 attataaaac gtggattaga cccacagcaa ggtttttaca tagggagaat agatgcaagt
      841 tgtaaaaaaa taatatcttt taagagtgta gttttaccaa attctgcaca taggattata
      901 aagaatagtg ctaatatata ctattattat aagtgtaatc tagataaatt cccaacaaga
      961 atatcaatag aaagtaatag agtttatact aatgtaaata aaattgtatt caaacaattg
     1021 aatataagta atactttaaa agctcctaaa aatctaagag atatattaaa agtaaatgta
     1081 aatagtaaaa tatttgatgt taaacctatg aaaaatctta taaacaaagg tagtaatttg
     1141 aaattatgta acttgatagt ttttgggtgt atagaagttg aaatagacta tagttgtaaa
     1201 aataggagta attttaaaac agtaaataag tctaatgaca ttaaaaaaga taagtattat
     1261 aaaaatgata tacaaaataa caatgaaaat ataaacgatt gtaaaaagca aataagtttt
     1321 ggtgataata aggtaaaaaa aataacaaaa acttttggat tttcttgttt catttgttct
     1381 ccagttggaa ttatttatga agatatgaaa aatataaata taaaaattga acatacatca
     1441 ataaatgaat taaatccaga agaattattt gtaaatacat cactt
//
LOCUS       DAEMIK010000298         1449 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEMIK010000298 DAEMIK010000000
VERSION     DAEMIK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.303"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            15..203
                     /locus_tag="KPK87_003839"
     CDS             15..203
                     /locus_tag="KPK87_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539910.1"
                     /translation="MEYMSCPEAAKKWGISERRVQILCREKRIQGVSKLGYMWLIPKD
                     AEKPIDGRTKQGKELYHE"
     gene            196..888
                     /locus_tag="KPK87_003840"
     CDS             196..888
                     /locus_tag="KPK87_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539911.1"
                     /translation="MNKVLIIDDDKELCALIKRSVLSEDIEADFCNTGKEGLQKLKER
                     EYQLVILDIMMPGMDGFETLEEIRKENSLPILMFTSKNDSASKVRGLRAGADDYLTKP
                     FDMDELIARIISLIRRYTRFNQQDGISQQLEFDGLKIDIENRSVTTENGTFELPPKEF
                     DLLLYCAKNQGKILTKQQIYEEVWREEYFYDDSNIMAIISRLRKKLEVNPGSPKYIQT
                     IKGIGYRFNKEV"
     gene            895..>1449
                     /locus_tag="KPK87_003841"
     CDS             895..>1449
                     /locus_tag="KPK87_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2539912.1"
                     /translation="MEAVIFLSIIIALFSILVSCFAIRRVKKQIAEITDALIDVKNGN
                     GNRRILSATNELVAPLAYEINEIVVSYESRLSTVRQTEETNRQLMTSLSHDVRTPLTT
                     LIGYLDAAHKGIVTGKDRDDYIETARRKAHDLKEYIDVLFDWFKLNSNEFAMDINIVE
                     AAELTRNILIDWIPIFEDKQIDYNI"
BASE COUNT          492 a          235 c          320 g          402 t
ORIGIN      
        1 ggacgggtgt tgttatggaa tatatgtctt gccccgaagc agcaaagaag tggggaattt
       61 ctgaaaggcg ggtacagata ctttgcaggg agaaacgcat acagggtgtt tcaaaacttg
      121 ggtatatgtg gttgatacca aaagacgcgg aaaaaccgat tgacggtaga accaaacaag
      181 gaaaggagct ataccatgaa taaagttttg attatagatg atgataagga actttgtgcg
      241 ctgattaaac gcagcgtact atcagaagat atagaagctg atttttgtaa tacgggaaaa
      301 gagggattac aaaaattaaa agagagagaa tatcaacttg tgatactgga cattatgatg
      361 cccggtatgg acggatttga aacattggaa gaaattagaa aagaaaacag cctgccgatt
      421 ttgatgttta catcaaaaaa tgacagtgct tctaaagtac gcggtttaag ggcaggagct
      481 gatgattatt tgacaaaacc ttttgacatg gacgaactga tagcccgtat catttccctt
      541 atacgccgtt atacccgttt caatcaacag gacggaatat cacagcaact tgaatttgac
      601 gggttgaaaa ttgatattga aaatcgttct gttactacgg aaaatggaac ttttgaattg
      661 cctccaaaag aatttgactt actcttatac tgcgcgaaaa atcaagggaa aattctgaca
      721 aagcaacaga tttatgaaga agtgtggaga gaggaatatt tctatgatga cagcaacatt
      781 atggcgatta tcagccgact tcgcaagaaa ttagaggtaa atcccggaag tccaaaatat
      841 attcaaacga ttaaaggtat cggttatcgt tttaacaagg aggtttgatt ttctatggaa
      901 gcagtcattt ttctatccat aataattgca cttttctcta ttcttgtttc ctgctttgct
      961 attcgacgtg ttaaaaaaca gatagccgag ataacagacg cattgattga tgtaaaaaac
     1021 ggaaatggaa acaggcgtat tttatctgca acaaatgaac tggtagcccc tcttgcctat
     1081 gaaataaatg agattgttgt gtcttatgag agcaggcttt ctactgtccg gcagacagaa
     1141 gaaacaaacc gacagcttat gacaagtctt tcccatgatg ttcggacacc gcttacaacg
     1201 cttattgggt atcttgacgc tgcacataaa ggaattgtta caggaaaaga cagggacgac
     1261 tatatcgaaa cagcccgccg caaagctcac gatttgaaag aatatattga tgttcttttt
     1321 gactggttca aattaaattc taatgagttt gctatggata taaatattgt tgaagctgcg
     1381 gagctgacac gaaatatctt gattgactgg atacccatat ttgaagataa gcagatagat
     1441 tacaatatt
//
LOCUS       DAEMIK010000299         1426 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEMIK010000299 DAEMIK010000000
VERSION     DAEMIK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.304"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            284..1156
                     /locus_tag="KPK87_003842"
     CDS             284..1156
                     /locus_tag="KPK87_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2539913.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT          588 a          152 c          230 g          456 t
ORIGIN      
        1 atacatctaa atatgcaaaa taagatagat aacttttaaa tgagtaaaaa ttgaaaaatt
       61 taaaagaaat attggataaa tattaaaaaa tgcaaggaaa gatggtataa taaatttaat
      121 cgaaaatact atgtgaagta taaggtacag aaaagttaga attttatgag tttaagaagc
      181 tatgataaaa actaagatga aattgagata aaaaataaaa tttttagaga aaaagacagt
      241 gttaataaaa caaaatagtt tatataacta aaaggagaaa aaaatggaag aaacaaatga
      301 aatgaaggac agtaaacatt tgatatcaaa tatacaatct caatatacga gattgagtaa
      361 gggtcaaaaa cttatagcac aatacatttt aaataattat gataaagttg catttatgac
      421 tgcatgtaaa ttgggtgaaa ctgtaggagt aagtgagtct acagttgtaa gatttgcaaa
      481 tgcattaggt tatagtggat atcctaaatt acaagctgca cttcaagaac tcataaaaaa
      541 taaactaact acagtacaaa gagttgaaat ggcacatgat tattccgatg attttgcaat
      601 actaaacaaa gttttaaaaa gtgatataga taatataagg tctacactag aagaaattga
      661 cgagagagcg tttaaagaag cttctaacaa acttttaaga gcgagaaaaa tatatatact
      721 tggaatgagg agttcttttg tagtagctca atatttaggt ttttacttag atataatact
      781 tgacaatgtt catataatta gaatggatat gggagatgct tttgagcaga ttgtaagaat
      841 aaatgaagaa gatgtaatag ttgctattag tttccctcgt tattctaaga aatcttatca
      901 aattgtcaat tatgctaagg aaaaaggagc ccatgtaata tcattaacag atagcttatt
      961 tgcgccagta gcatcacttg cagataacac tcttctagtt aagagtaata tggcttcatt
     1021 tgtagattct ctagttccag ctcttagtat ttcaaatgcc cttgctatat ctgttgggat
     1081 gaaggaaaaa gaagatatta aacagcattt tgatgattta gagcaaattt ggaaaagata
     1141 ttctgtgtat gagtaaagtt tgtatattaa taaggctatg aaattgagaa tcatagcctt
     1201 attagtaaat actcgattat cacttaaaaa ataaattttc gtttaaataa tacaaataaa
     1261 tatttcgaga ctcaaaacat aaagatgtat tctgaataca tctttactac gtcaactaat
     1321 ttattaaaga aagcatttcc attcatggag agtgcttttt ttatttctct aaacaatatc
     1381 atttccttaa ttaacaatta taaaattaat tttatttgta tttaag
//
LOCUS       DAEMIK010000300         1348 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEMIK010000300 DAEMIK010000000
VERSION     DAEMIK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.306"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(601..1194)
                     /locus_tag="KPK87_003843"
     CDS             complement(601..1194)
                     /locus_tag="KPK87_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2539914.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          481 a          194 c          170 g          503 t
ORIGIN      
        1 agtatagaaa attaatctaa agaagtataa taaactaatt taagaaatat agaaaattaa
       61 tttaaagaaa tacaatacat taatttttaa gtaacattaa attaatgtaa gtaaaataat
      121 gttcatatga agatttaaag gtgcattttc tgaagctaag tatatgttgt agagtgttat
      181 gtctctctat ccagtgaagt ttaatagctc aagaaaatgc actatttatg atgattattt
      241 tgttttattt ccaattttgg aaataactat tctaattaaa tctaaaatta taccataaat
      301 aagaaaagaa agtatagatg ctatgtacca ataaatcgaa attgaccctg taattgttct
      361 acatgggatt tgcatacctg aatccataaa acaaaaaaac tatcaactct ggaaataata
      421 gacaaaatag gatttaaacc tattagaata atactttttg agtcttgacc aatttgatgc
      481 atataaatct caaataatgc aattaaaaaa aaccaaactg taaaagattt aataattttt
      541 ttcatcaaaa tacttcacat aatataaatt ctgtttgcac tatttgctta ctacaactat
      601 ttatctccat tcagtcacat cacttaattg taatctaggt cttcttgatg gttcttcatc
      661 aggataaccc aaagatattg ctgccactaa ttgactatct gtatttaacc attcacataa
      721 ttcacgatat gcaaagtata catcacaaat ccaaagacta cctagaccta attccacagc
      781 tgtaagcgac atattctgaa ttgcagctcc aattgattgc atgtttgcca tttcatagaa
      841 tcgttcttca ggggatagct tttctaaagg tgatttacct aatatattta aaataaaaat
      901 tgttactgga gcttgtttca tgatttcaac tgtgtaattt gcaccagcga tatgttgacg
      961 acttcctgga agaagcccat tatcattaat ttcattttga atacctttgc tcatggcttt
     1021 aagcatagat tctttttcct tttcagtgat gacaacaaaa cgccatggtt gcctgttttt
     1081 agatgaaggt gcatttattc cagcttcaat tattttctct atagtttcat gtgaaatgct
     1141 ttgattttta tattttctga tacttcgtct ttttgatata gaatcactta tcattttaag
     1201 taacctccgt ttattttttg gaaaaatcta tttaattttt attttctatt ttaattattt
     1261 tagcattaat cagaaaaaaa gtcattgatt ttagtataca attattaatg attatattaa
     1321 gaattgtatc aaaaagtatg tgattata
//
LOCUS       DAEMIK010000301         1340 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEMIK010000301 DAEMIK010000000
VERSION     DAEMIK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.307"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            171..>1340
                     /locus_tag="KPK87_003844"
     CDS             171..>1340
                     /locus_tag="KPK87_003844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539915.1"
                     /translation="MLNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKNINN
                     THITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLGFSD
                     LKNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAETDY
                     PGNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRKRIE
                     SVNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLINKN
                     ICTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNLDFL
                     EETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDNN"
BASE COUNT          544 a          176 c          146 g          474 t
ORIGIN      
        1 atatccatta tattcttgat aatgatatat gtgaaattta ttttaataaa caaaggctaa
       61 caaattctaa gtattatcaa gataatatta atgtattaaa ttatattata aatgaaaaaa
      121 ataataactt aattatcaat tataatgaca aatacatata tgctatttat ttgttgaata
      181 tagctataaa ctcatactta aataatacat taaacccaaa taatcacatt cttacaaaac
      241 tcgataaagg tatgcccctg ctctacaagg gtcaaatagt tatatttaaa aatataaata
      301 atacacacat aactttagga cataaaaata aaacggatac aacacttata cctattgact
      361 cggcttatat gcttataacc tataatggtc atttagaagt caattctatg ggcaaaactc
      421 cagcatctaa gacttttcaa acaaagaatg ttctatcatc tttaggtttt tcagacttaa
      481 aaaatatgac tggagtaata aatgattcta cactgattat attgccttgt aaagatgata
      541 tatctgactt agttagtaat attaaaataa aagatattaa taatacttat aagtttactg
      601 aactatttcc atgttcatat atttcatcaa ctggtgccga aactgattat cctggaaatc
      661 acgctaagca aataccttta cttaaattta caactaatat ttctagtgca tttgaaatta
      721 ttaaatctga taagtctata aaaaatgtat ttataatagg tgattatcca atttataagc
      781 atataaatga ttttgaaaga attttaaata gaaaaaggat agaaagtgta aatattgtaa
      841 cctcaaattc aaatataagc aatatattag atttaccaaa tatagataat ctaaatatat
      901 actcttggtc taaagatgtt ctgttaacct atagccaaga ttttgaaaat acctctactt
      961 ttcagaaatt atggctagat aaacttataa ataaaaatat atgtactact tctgttgatt
     1021 ctaatatctc tgacttgata catcaggcta gaaaatcact ttattacata tctaagtatg
     1081 attttgatat ttccataaaa aactcactta tgatgtgtag ttactctctt cttaaaatct
     1141 tagaaaatac accttttaac ctagattttt tagaagaaac tataaagctt ttaaatttaa
     1201 atatagcaac tccttcagcg aagctagaaa atataaaata tttaatttca agtactagcc
     1261 cagatttaga attaagtgca ttatttgatg atgttatagt caaatttgaa agaattataa
     1321 atgaattatc tgataataat
//
LOCUS       DAEMIK010000302         1337 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEMIK010000302 DAEMIK010000000
VERSION     DAEMIK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.308"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPK87_003845"
     tRNA            27..101
                     /locus_tag="KPK87_003845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPK87_003846"
     tRNA            116..200
                     /locus_tag="KPK87_003846"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPK87_003847"
     tRNA            210..293
                     /locus_tag="KPK87_003847"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="KPK87_003848"
     tRNA            323..399
                     /locus_tag="KPK87_003848"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="KPK87_003849"
     tRNA            407..482
                     /locus_tag="KPK87_003849"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="KPK87_003850"
     tRNA            571..659
                     /locus_tag="KPK87_003850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="KPK87_003851"
     tRNA            663..738
                     /locus_tag="KPK87_003851"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="KPK87_003852"
     tRNA            745..818
                     /locus_tag="KPK87_003852"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="KPK87_003853"
     tRNA            833..909
                     /locus_tag="KPK87_003853"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="KPK87_003854"
     tRNA            933..1009
                     /locus_tag="KPK87_003854"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="KPK87_003855"
     tRNA            1017..1093
                     /locus_tag="KPK87_003855"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="KPK87_003856"
     tRNA            1102..1177
                     /locus_tag="KPK87_003856"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="KPK87_003857"
     tRNA            1185..1258
                     /locus_tag="KPK87_003857"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="KPK87_003858"
     tRNA            1265..1336
                     /locus_tag="KPK87_003858"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAEMIK010000303         1336 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEMIK010000303 DAEMIK010000000
VERSION     DAEMIK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.309"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..1085
                     /locus_tag="KPK87_003859"
     CDS             192..1085
                     /locus_tag="KPK87_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2539916.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT          562 a          130 c          191 g          453 t
ORIGIN      
        1 taaaataagc ttatctaatt atttatttta actgttttgt gtgatagaaa actatatatt
       61 ataacaatag tagtgctttt aattaaatat atttcataaa tcttcataaa atcttcaaaa
      121 tttcgtaaag aatatgtcac aaaagtaagg tatattatat tcagcagata gtataaggga
      181 ggacaatatt tatgaagaat aaaaaattca ttgttataat actgataata tcaattttaa
      241 taggtgtatt agtgaaggaa tattcatcta gagagataaa aaaggatgat ataaatgtca
      301 gtaagtatat aaaatatgct gatttagcaa gtaaaaataa tgcacaagtt aactggaagt
      361 atgtagcttc cattgttgct gtgttaaata aaaataactt aaaaaacgtt aaggattcac
      421 agatacaaga agtatcagat ttatttgtta agaatttttc taaaaataat aaaataaata
      481 agttaagtga tattttggat gagttagaat ttagtaatag acaaaaaaga ttggttgata
      541 attatataga caatcttaag gattatggta taaaaccaga aagattgaag tctgatacaa
      601 aatatatgaa gtttattgat gaaattaaaa cagaagcaat acaaaattat aaggattata
      661 aaatacttcc atctataact attgcacaag ctataataga atctagctgg ggtaagtcaa
      721 ctttggcaaa acaatataac aatctatttg gtataaaggc tgacgcttat tggaaaggta
      781 agtcagtgac tttagaaaca aaagaacatt tagatacaat tatagatgat aaatttagaa
      841 tatatgatga taaaaatgaa tctataaaag accatgcaaa atttttagcc acaaataaaa
      901 ggtataaaaa caatggtgta tttgatgcaa aaacgtatat atatcaagct aaagcattag
      961 aaaaggcagg ttatagtact gcgaaggatg aaaatggcaa tagtatatat gcagctcgtc
     1021 taatagaatt aattcaacag tataatcttc aattaataga cagtgaaatt caaagtgaaa
     1081 tgtaactttg attaaatttt tattgataag gctatgattt ttaaaatcat agccttattt
     1141 tgacatgttt ttacttttaa ttattaaatc gatatcatta tctttcaaaa tttactttat
     1201 actgtaaatt aaattattta agttaaaact tggaattatt tataaaagga ttatttactt
     1261 tatctaaatt ctgcactcaa agaacatctt ttcccttaag actgaccgtt agtctataaa
     1321 aattataatt acttat
//
LOCUS       DAEMIK010000304         1306 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEMIK010000304 DAEMIK010000000
VERSION     DAEMIK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.310"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            481..855
                     /locus_tag="KPK87_003860"
     CDS             481..855
                     /locus_tag="KPK87_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2539917.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            960..1306
                     /locus_tag="KPK87_003861"
                     /pseudo
     CDS             960..1306
                     /locus_tag="KPK87_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          584 a          125 c          171 g          426 t
ORIGIN      
        1 ctacattttt ttatgctcta gtataaaatc atctaactgt gtatctataa aatcaaaata
       61 attatatgaa ctatcagtat aataagtcaa aatatgtata catgaataca tattttaaaa
      121 gatatatatt attacatacc taaaaaactt tcataaaaac aaaaataaaa tatgctttta
      181 gtaacttgtt aacataactt ataatttatt ttataagcat tagcctatat agctttgtgc
      241 tactaaatct ctttgattct aacgatttag tgctattcta caactaaaat aatacaatag
      301 agctatttat tgtttggtag cattgtttgt aaattaaaca aatttataca tatgaatatt
      361 attttacgtt caaaaaacag actaaatatg tagaatattt ggtaaaactt acctctagtt
      421 tttgtcttaa aaagctccta ttattaactt aagttagaag aattaaggaa gtgacataaa
      481 ttgaaagaag aaatattaat agaaaaatta ttaagagcag aattaatagt aatggagtat
      541 ttatggaaaa aagacactct aatgcctaaa aaagaaattg taaaagaaat aaaacagatt
      601 tatggatggc ataaaagtac aataaaaata ctactaaaaa ggctagttgc taaaaggtat
      661 ttggctagat atattataaa ttctcaatct cattataaaa taatagatag taaagaatat
      721 tatactttta agaaaaaggt actaaaatct agtaaaagca taaaaataat gcgttcactt
      781 accactacac ataaaaatgt aagtaaagag aagttagacc tattagatga gtattataga
      841 aacttaaaag aatgatatag taaaaatcga atgtaattat ttaccttaat tcttttgttt
      901 gtaaaacttt attgatacag aatttaacat atacataaat tggtaaagga atgtatacta
      961 tgaaaattag taaattacca gaagcggaat taaaagttat gagatatatt tgggaatcag
     1021 aaaaggtatt aatatcaaga gaaatcacca agtctatgga acaaaaatat gaatggaaag
     1081 atacaactat atttacagta ttaaaaagat tactaaagaa gagagttttt agaaacggag
     1141 aaaatagata agtgtacaca ctataatgta ttagtaaaaa aaaataatat cagagatttt
     1201 aaacaaaaga atttttaaag aatacacata aaaattcaat attaagttta atgtcagcac
     1261 tatgtagtaa ggatgatgaa gtagatggtg atattttaaa tttatt
//
LOCUS       DAEMIK010000305         1279 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEMIK010000305 DAEMIK010000000
VERSION     DAEMIK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.311"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            228..1181
                     /gene="blaCDD"
                     /locus_tag="KPK87_003862"
     CDS             228..1181
                     /gene="blaCDD"
                     /locus_tag="KPK87_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG2539918.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEDAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMI
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          548 a          101 c          218 g          412 t
ORIGIN      
        1 attatatcac aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc
       61 aataggttta atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca
      121 tttagtcttg tttcttataa gtagaaccta gattggagta tattacaaat aatgtatact
      181 attttaatag taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa
      241 agaattttat ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag
      301 ggaaaaaaca taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa
      361 ataagtctga attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa
      421 ttaatatggt tgactatagt gattgttttg aaggtattag tggaggagct attttttata
      481 atactaaaaa taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac
      541 catgttctac ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact
      601 caaaagaatc agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa
      661 atcttagttt agaagatgca tttaaggagt cttgtgtttg gtactacaaa aaattgatag
      721 ataaagttga tgctaaatct gtccaaaata ttttagatga tttaaagtat gggaattgtg
      781 atattagcga atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt
      841 tagaatcttc attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg
      901 agggagatac aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag
      961 atgtaaatga taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa
     1021 ataaacgtgt ggatgcatgg tttgttggta tgatagagcg tgaaggtgac acctattatt
     1081 ttgctattaa atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg
     1141 caattaatat aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa
     1201 tattatttac ctatagtctg taatatttat taaataattt tactaaattg aatctatata
     1261 gagcaaaact cctttagta
//
LOCUS       DAEMIK010000306         1267 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEMIK010000306 DAEMIK010000000
VERSION     DAEMIK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.312"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1267
                     /locus_tag="KPK87_003863"
     rRNA            <1..>1267
                     /locus_tag="KPK87_003863"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          327 a          279 c          389 g          272 t
ORIGIN      
        1 tgattagcta gttggtaagg taacggctta ccaaggcgac gatcagtagc cgacctgaga
       61 gggtgatcgg ccacattgga actgagacac ggtccaaact cctacgggag gcagcagtgg
      121 ggaatattgc acaatgggcg aaagcctgat gcagcaacgc cgcgtgagtg atgaaggcct
      181 tcgggtcgta aaactctgtc ctcaaggaag ataatgacgg tacttgagga ggaagccccg
      241 gctaactacg tgccagcagc cgcggtaata cgtagggggc tagcgttatc cggatttact
      301 gggcgtaaag ggtgcgtagg cggtctttca agtcaggagt gaaaggctac ggctcaaccg
      361 tagtaagctc ttgaaactgg gagacttgag tgcaggagag gagagtggaa ttcctagtgt
      421 agcggtgaaa tgcgtagata ttaggaggaa caccagttgc gaaggcggct ctctggactg
      481 taactgacgc tgaggcacga aagcgtgggg agcaaacagg attagatacc ctggtagtcc
      541 acgctgtaaa cgatgagtac taggtgtcgg gggttacccc cttcggtgcc gcagctaacg
      601 cattaagtac tccgcctggg aagtacgctc gcaagagtga aactcaaagg aattgacggg
      661 gacccgcaca agtagcggag catgtggttt aattcgaagc aacgcgaaga accttaccta
      721 agcttgacat cccaatgaca tctccttaat cggagagttc ccttcgggga cattggtgac
      781 aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga
      841 gcgcaaccct tgtctttagt tgccatcatt aagttgggca ctctagagag actgccaggg
      901 ataacctgga ggaaggtggg gatgacgtca aatcatcatg ccccttatgc ttagggctac
      961 acacgtgcta caatgggtag tacagagggt tgccaagccg taaggtggag ctaatccctt
     1021 aaagctactc tcagttcgga ttgtaggctg aaactcgcct acatgaagct ggagttacta
     1081 gtaatcgcag atcagaatgc tgcggtgaat gcgttcccgg gtcttgtaca caccgcccgt
     1141 cacaccacgg gagttggaga cgcccgaagc cgattatcta accttttgga agaagtcgtc
     1201 gaaggtggaa tcaataactg gggtgaagtc gtaacaaggt agccgtatcg gaaggtgcgg
     1261 ctggatc
//
LOCUS       DAEMIK010000307         1252 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEMIK010000307 DAEMIK010000000
VERSION     DAEMIK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.313"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            74..>1252
                     /locus_tag="KPK87_003864"
     CDS             74..>1252
                     /locus_tag="KPK87_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG2539919.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLYELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSSISFSLDDTIFKTLISY
                     D"
BASE COUNT          533 a          160 c          152 g          407 t
ORIGIN      
        1 aaaatttaaa ttttatacat attataataa attgtgatat atataaaata attaaagata
       61 aaaggagtat tttatgaaca tacttggatt aatagttgaa tataacccat ttcataatgg
      121 acacatatat catttatcta aatctataga aaaaacaaat gcaacacata ctgtagcaat
      181 aatgagtgga aattttctac aaagaggcga accagcttta tttgataaat acactagagc
      241 aaaaattgct gtcgaaaatg gtgtagactt agttatagaa ttaccaactt tatttgcttg
      301 tcaaagtgct gaaatttttt ctcatggtgc tataacaatt ttaaattcac taaactgcat
      361 aaattctata tgttttggta gtgaagaagg aaatacagaa attctttata atctagctga
      421 aattttagca aatgaaccaa atgaatttaa gttactttta aaaaaatatt tagatgatgg
      481 tctgttattt ccaacagcta gagctaatgc actttttgat tatatacatc aagacaaaaa
      541 aaataatttt atacaaaatt attcaaagga acaattatca aaaacattaa attcatcaaa
      601 caacatactg ggtatagaat acataaaaaa cctcatccat ttaaacagta gtataaaacc
      661 atttactatc tccagggtcc aatcagaata taattccgaa gaaattgaga gtgatatttg
      721 ttctgctact gctataagaa cttcacttaa agaaaataca gacctttttc atttaaaaaa
      781 tgttattcca gaaaaaactt ataacatcct aaatgaaaaa atagataatg gttttttacc
      841 tatgtttgat gacttcttct ttgatatttt aaaaacaatt atacttagag atatggacac
      901 tttaaaagag tattttgaag taaatgaagg catagaaaac agaatctata aagatatctt
      961 tacatctgaa tttctatatg agctacaatt agcaattaaa tccaagcgat acactttaac
     1021 caaaatcaaa cgaactctaa acaatatatt gttaggtatt aaaaaaagcg acatgcaact
     1081 tataaaaaac atagatacta taccttatat aagaatactt gcttttaatg ataaaggtag
     1141 agaaattcta aaagagataa aaaataaatc tgaaataaaa ataataaaca agttttcaag
     1201 tatatcattt tcattagatg atacgatttt taaaacactc ataagttatg at
//
LOCUS       DAEMIK010000308         1225 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEMIK010000308 DAEMIK010000000
VERSION     DAEMIK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.314"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            197..637
                     /locus_tag="KPK87_003865"
     CDS             197..637
                     /locus_tag="KPK87_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2539920.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(822..1010)
                     /locus_tag="KPK87_003866"
     CDS             complement(822..1010)
                     /locus_tag="KPK87_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2539921.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
BASE COUNT          449 a          183 c          136 g          457 t
ORIGIN      
        1 tatattttat cattatttta catttttttc caatatatat aattatcata tttttctatt
       61 agctaaaact aattacctta tatttttatg tattggtata ataatacata aaaacttgaa
      121 taatgcataa aaacttgaca tactgttgtc ataattactt tgctataatt aaaagtaaaa
      181 atggaggtaa attctaatgt atgaacgtat gcttgataaa agcaataaac cctgtttgtc
      241 ccaaataata gaatatatag gtaaggatgg atatgcacga ttatgtaagt tggaaactta
      301 cttgaaaact caatataatt tatcaagaga acttcgattt ccttttggaa acaattatgg
      361 ttggggttac aaatatagcc ataaatcttt tcatttattt tatgcttttt ttgaaaaagg
      421 tgcatttact gttctgattc agataggtga caagcaagtt ccagcagtag aaaaaacgtt
      481 ggtagctttg acaccaaaag ctaaagagct ctgggtcact cgttatcctt gtggcaataa
      541 tggaggttgg attaagtatc gtattttaga agatgcagac ttacttgata ttattcaata
      601 tataagagca aaaaaatctc cacctaaaca aaattaactt ttattttttg tgatttaaat
      661 atagtgtata taaattccat tctttccata taaaaaaaat aatataaaaa atatctatta
      721 gtataactca tttagtatgt cttattctac tttttacaga taattaaaaa gttttatcat
      781 aaataaatca taactagaca tcctagtaaa tcaaaattaa tttattcttt tttattcgaa
      841 tttttgataa tagtccctat acatatgcca attatcattc caaaaattag accataacac
      901 attgtgatac ctacatttcc tttagaaata cctatagcac aacctatagc cattccaagt
      961 atcatgccta taaacataca tataataact aaattgttgt ttttattcat agtttttctc
     1021 cattcaatat attttaaatt gcctagctcg ccttcttagc ttaaaacaaa gaaatataaa
     1081 ttaactttct ttcattctaa tatctgcctg tataaatgtc aaacaacaca tttcttgtca
     1141 taacctcctc tttttgaagt aagtacaatc tcccagacgc aataatatca atttcctaaa
     1201 accatttaag taacttttat attta
//
LOCUS       DAEMIK010000309         1201 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEMIK010000309 DAEMIK010000000
VERSION     DAEMIK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.315"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            85..>1201
                     /locus_tag="KPK87_003867"
     CDS             85..>1201
                     /locus_tag="KPK87_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2539922.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYK"
BASE COUNT          462 a          126 c          166 g          447 t
ORIGIN      
        1 aatatatcca attaaggtat aattaagttt tatatgatat agttatattt gataatttac
       61 aatagtaaaa cttggaggga aaaaatgggt gaaaagaaaa taaataatat gaattattca
      121 cataataatt attccaaaaa gacaggtgaa ccaactagaa aaataaaaaa tagccatatc
      181 actcttaaaa gtaaagaaga aaaaatttgt tctagttgca ataaagaaaa taatactaag
      241 gataattact gtaaattttg tggaaatgaa ctttatgaaa tagcatcatt aagaccactt
      301 gaaacaaaac tagacttgaa atctaaaata aaagagcttt catatcatgc aaataaaaga
      361 ggtgtatttt taacaacatt tacaaccata tttatattgt ttattattgc attgatattt
      421 aaagctatta taactattca atttaatgat attagttatt tagtaaatcc agcacatata
      481 atcctagctc tcaacttggg tcaaattagt gtatctatgt ctaccatgat gggttctggg
      541 tttataaatg ccaatatagg tttgttgata ttacttataa tccctatttt ggtattatta
      601 atatcaaacc taatttttat gagaaaaaga tgtagagatt caaaaactgt attagcaaat
      661 tctctaggag ttggaatttt ttatggatta gttttagcta ttttatctat ttttactaat
      721 gtaaaaacta gttcacatag tatgttagaa tatggatatg cattagaata tagttatgaa
      781 ttttttagtg ttcttttaaa tggattcgta ttagggttta tatgtacata cataactact
      841 tataaaaaaa gttatgaaaa agaaaatatg tatttatctc ttttttcaaa ttctataaga
      901 acattcatat taggttatgt tttagtatta gtaatactat tggtactaac tatatctgat
      961 agtagttact taaatgaact agatatgagt agttattcaa atggattaaa tttatttact
     1021 atactcccac aaatagctag ttatatgtgg gcttttgcaa atggaatttc agtaaccata
     1081 ataaactcaa cagtatcaat gtttacttta agttcttcat ctttatttgg agatactaaa
     1141 ttaatgtttt atgcaatggg agcattgtct atgttaatat tattattaaa tggatacaag
     1201 c
//
LOCUS       DAEMIK010000310         1162 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEMIK010000310 DAEMIK010000000
VERSION     DAEMIK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.316"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            45..776
                     /locus_tag="KPK87_003868"
     CDS             45..776
                     /locus_tag="KPK87_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539923.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(933..1139)
                     /locus_tag="KPK87_003869"
     CDS             complement(933..1139)
                     /locus_tag="KPK87_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539924.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          485 a          124 c          146 g          407 t
ORIGIN      
        1 tatttttcac aagtacaata cttatttaaa aaaggaggtt taatatgtta agtaaaaaaa
       61 atgtaagttt aattatttta gtaataattt taagtttatt aactgctata tttattttca
      121 ataataaaaa tactaacaat aaattgaaag aagttactaa gaataatgaa gaaatagtac
      181 ctaaaaactt agaaggttta agtatagaac aattaataga gattagagat aaagccattg
      241 ataaaaattt agaaggtctt agggaatttg gagaagattc agtaaaaaaa cgtaaagatt
      301 caattttaaa ctttgaattt ggattagtag cttttgttaa caagaattat agcaacttaa
      361 cagaaaaaga gaaattaata agacaaatta atattgttaa ctatgaaaac tctataaatg
      421 atataatgga tccttcaggt tcattctttg aattagcaaa tcagaattac caagtgaatg
      481 agtttctatt aaaaaacgga aaagattata aagaatggtc atatgaaggt ctagattttc
      541 taattgatca tctagaagaa ctgtgcaaat ttgttgattt tgatgtaaaa gatgtaatag
      601 atattattga tcctacaata aaaacagacc tttcagagga acaaaagaaa tctttgaatg
      661 ataaattaaa gaaaatatct tcaagtgata ctttaagtga agaagttaaa aaggagataa
      721 aaaactggga aaacaattta aataatttaa atatgaataa aaataattct aattaatttt
      781 ctcataagaa aaatgcttat atcgtgataa tgtgaaatgt acataattat aaacaaaaaa
      841 ggggctgtga cactaaaaaa tagtgctacg ccctttttta tttgtaataa tcatcaatac
      901 aaattatttt attatatgta attatttatg tcttaaccaa ttttatcgaa aataacagtc
      961 ttctctttta aagagatttt tcctacttga tatccatatt catttaattc ctttttatat
     1021 ttcaaaaacg aatggtctat ctctatccca gcaatatctt ttatttcttc aaaagttaat
     1081 ttaaacgatt gactattgtt tttcttaaca tattcccata aagaactata tttactcatc
     1141 ttatatcact ccttttaaaa tc
//
LOCUS       DAEMIK010000311         1099 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEMIK010000311 DAEMIK010000000
VERSION     DAEMIK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.317"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            48..377
                     /locus_tag="KPK87_003870"
     CDS             48..377
                     /locus_tag="KPK87_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539925.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            381..>1099
                     /locus_tag="KPK87_003871"
     CDS             381..>1099
                     /locus_tag="KPK87_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539926.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFA"
BASE COUNT          472 a           76 c          208 g          343 t
ORIGIN      
        1 aagtactata cattatagag tagtaggtga aaaggcggtg gtacataatg cagttaaata
       61 aagaagtgtt aaaaggttat atcgatatat taattgtttc tatattagaa aagaaagatt
      121 gttatggata tgaaattgca aagcaagtaa gagaaagaag tgagtttgaa ttaaaagaag
      181 gaacaatgta tcttgcactt aaaagaatgg aatctaaaaa tttaattaaa tcttattata
      241 gcaatgaaca aagttcaggt ggaagaagaa aatattataa tttaactaat gaaggtaaag
      301 attttctaga aataaagaaa caagaatgga gatttattaa aaaggttatg aatcaatttt
      361 tgggagaggt ggattaattt atgaaactaa tagatgaata tttagataaa ctatataaaa
      421 aatgtgacaa taaaagtact atagaattaa aacaggaaat gagatgccat cttatagaat
      481 ctgcaaatga atttaaatta gaaggattag atgaagaaga agcatgtaaa aaagcaattg
      541 aaagatttga tgatggtgat gaaatgcagt atgaattatg taatataata aaagaactat
      601 ctttatcatt ggataggcat aaatcaattg taatggggtt taaaaaagta ttaggttaca
      661 taagtataat tgcattttta atttctggat ttatgtggta ttacaataat agtttacaac
      721 ataatatgta taatttagga aaagaacttg atggagaaat aaagcaattg gcagaaagac
      781 atgatatgac aaagattggt gaatataagt tagaacttga aaaaatactt gataaagata
      841 agtatagtaa agtcaaagct cttagactat atgtaataga tatgaaagat ggaaacacta
      901 atttaagttc ttctggattg aatgctaata tggtatatga gagggaagca gattataata
      961 acataagtaa ttttatacag catttaggat ataatggaaa agatttttta gacaagaatg
     1021 ggaatatagt aaatccagat attttcttag aatatttctt ttattttgaa tcggagatgt
     1081 taattccagt agcatttgc
//
LOCUS       DAEMIK010000312         1083 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEMIK010000312 DAEMIK010000000
VERSION     DAEMIK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.318"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..182
                     /locus_tag="KPK87_003872"
     CDS             <1..182
                     /locus_tag="KPK87_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539927.1"
                     /translation="AVFESKIDTVKIGDYENNTRAIQVLDNIQNFIENGTKYDYITSN
                     KVRYNVNKIFQMPAK"
     gene            complement(292..972)
                     /locus_tag="KPK87_003873"
     CDS             complement(292..972)
                     /locus_tag="KPK87_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2539928.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          436 a          141 c          114 g          392 t
ORIGIN      
        1 atgcagtatt tgaaagtaaa attgatactg taaaaatagg agattatgaa aataatacaa
       61 gagctataca agtgttggac aatatacaaa acttcattga aaatggaaca aaatatgatt
      121 atataacatc aaataaagtc agatacaatg ttaataaaat atttcagatg ccagctaaat
      181 agtgagtgtg cattttaatt taataactaa agcccacttt cttttgtagt atgaaagagg
      241 gctttacata tttgtaaata tcgaagtaca cttattttat caataaatta tttaataagg
      301 tataatggac caattaaaca ataaaaattc tcctaaaaca gcaagtatta aatatataat
      361 agcttcattt ttgtaaagag aggtaatttt tttgcttatg tcatcatcaa ttgtaatagc
      421 aatctcatct tgaataatat ttttatatat agatgggaaa atatcttttt tctttataag
      481 attaaataaa ttatttacac ctacccatat aaaatatcct aaaacagcac ctaatgtgtt
      541 catcattaaa tcatcaatat ctgtagctct taagttaaac aattgacaaa gttcaattgc
      601 taatgaaaaa cctaatccag ttaatgaaac attgataata tttctaaaat ttttccaaat
      661 taaaggtagt aaaaatccta aaggcataaa cataattata tttaaaatat atgttaagat
      721 gccaactgag tcaaaaggta ttagatttat ttcatcaatt ctaaaaagcc cactatacct
      781 tcctatatcc catatgctac caattccagc tttactaagt gctagataga tatataataa
      841 aaaaatatat acccatacta aatgtatcat tacattcttt tcaactttca aattttttcg
      901 ttttataacg actatttggt atattaagca tggtaatata acgcaaataa atgaataaaa
      961 tgtccatatc attttatttt gtcccctttc aaatttatgt ttttagtttt ataagtacta
     1021 tctttatatt aaactgtaag ttttaaaaat cttttaataa aatcttaaaa atttattaat
     1081 tta
//
LOCUS       DAEMIK010000313         1073 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEMIK010000313 DAEMIK010000000
VERSION     DAEMIK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.319"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            375..551
                     /locus_tag="KPK87_003874"
     CDS             375..551
                     /locus_tag="KPK87_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539929.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          416 a          128 c          112 g          417 t
ORIGIN      
        1 ggaaatttta ttgttgttca aattgtagtt attctttaaa tttaaagatg gagtttttta
       61 agaatagaac ctactgtcct tattatggaa agattattaa atagttgcat ataactttat
      121 ttatcttgaa tcttattttt aaaaacaatt ataattttag aatattttct attctcaaac
      181 aagcatgttg aataatgaag catcttctag tattatatac ttgtagaata aaactaaatt
      241 ggtaaaactg ggaatattta accttaagga gttaagttat agaaactagt actaaaaaat
      301 tttataaatt atgttaattg actaatagaa tgcgactttt tatttttact aaatttttta
      361 gggggatgga taatatgttt attggagaaa tagacaaatg tacacatata ctaactgctt
      421 acatcagtag ttcatatgat tattgtaatt ttttagatac acagttagat gattttatat
      481 cagaatatgg agaaacggta gttgaaacct gcttacatca agtcttatta ttagtaagta
      541 gatacaatta aaactaaatt tttctagaaa aaccttttta gatttatcta ctctaataaa
      601 tatataattt tttgtacttt ataaataact ataacttctg ctaaagtaaa gatgtattgg
      661 gagtacatct ttactttccc attatagaag ttaaaagcat acaccataaa attaatcatc
      721 caatacaaat tatatatttc ctatgtctaa cttaatttac aagaatcatt ctatatgcat
      781 attctgcaat ccactaaaca tggctctaaa cctttactat tttatacatt ctctataaat
      841 catacaaatc atatataaaa tccccctaat atgtttaaac cacctataaa ataacaccaa
      901 tatttagaat attcaatatc aactgcataa ctggtcaata aaaacgtata actagacaaa
      961 tttcaatatt tatataaata atacaagaaa aacaagcatt ttatttatat aagtttttta
     1021 ttttttgttt atattaaaaa actatattga ttaatttatt ttatttagta att
//
LOCUS       DAEMIK010000314         1064 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEMIK010000314 DAEMIK010000000
VERSION     DAEMIK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.320"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            41..295
                     /locus_tag="KPK87_003875"
     CDS             41..295
                     /locus_tag="KPK87_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539930.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            340..663
                     /locus_tag="KPK87_003876"
     CDS             340..663
                     /locus_tag="KPK87_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539931.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          437 a           82 c          171 g          374 t
ORIGIN      
        1 aaaaataatc aaataaattt agcttatgag agggataact atggaaaata aggattttaa
       61 attaacaata tgtatatttg cttttgtaat tggagtagta gtattattct gttgtatagg
      121 tttaggtgaa tttagtgcag agcatattat tcaacaaaat ggtggaatca tggatactga
      181 ccaatataat atttatctag accaatctat ttctcaatat agaaattttg gttctatatt
      241 ggctctgtta ggaggaatag ggatattgtt tgataaatat atcagaaaat actagagatt
      301 atgttttgtc taaaataaat tcttatgagg agataaaaaa tggcaacaga aatatttact
      361 gtaaaaatta cacccttaga ggcacaaaaa atgataaaag aaaatcttga tgcagattta
      421 gtttttagtg attcttataa tttaggagat gataaattta tactaattac aacttttgag
      481 aagtattacg caagaaataa tagtgatgca ggattaatag tagtttatga aaataccaca
      541 ggaaaaacag ttattaaagc tacttcaaca ggcagtgcaa ttggaatttt aaaaattgat
      601 tggggtgcag gtggaaattt acttaaaaga gtaaaaaaca tacttgctga ttatattatt
      661 taattatttt atctaactga taatatgtca agaaaatctc gtaaaagagg ttaagaacat
      721 ttttataata tatagataaa taagaatttg aaggtaaagt ttttaaggta taaagaaaaa
      781 taatataata aaacgaattg gctttataat tctttataag gcaaagagca catttcttta
      841 tataattgtt ggatattaaa taatatgtaa atacaccatt tacatataaa agtttaatta
      901 atatgttaag agaaaatgaa atggaaatta ctaaaaaata tttttaaatt ggataatggt
      961 tgatattatg tttatacaaa aatcatgtag aattttatat attttaaata tgatagttta
     1021 aaattatgaa agaatgataa attccagttt gtagggtaat tttg
//
LOCUS       DAEMIK010000315         1035 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEMIK010000315 DAEMIK010000000
VERSION     DAEMIK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.321"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(225..941)
                     /gene="dapD"
                     /locus_tag="KPK87_003877"
     CDS             complement(225..941)
                     /gene="dapD"
                     /locus_tag="KPK87_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG2539932.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
BASE COUNT          361 a          185 c          115 g          374 t
ORIGIN      
        1 atattgaaaa attgaaggaa ataaaaatac ttgtcagcaa gaaataaaaa tgatataaga
       61 atgaataaga gaattgtaaa aaaataaaat gattataaat aaaagataaa aatattgtaa
      121 aaaagagggt gcctctggtg aattaaattc acacagagac acctttttaa ttaatttttt
      181 aaattagtat attattgttt aattaatagc atagtactat ttaattaatc taaatttcta
      241 aggtcttcca taagcttagt tttgtcagcc gttttttcat ctttcatttt tataacttta
      301 gcaggagaac cagcaacaac ggcaccagct tctacatcag tagttacaac agcaccagct
      361 gcaacaacag cacctttacc gattctaaca ccttcaagaa taactgcatt agctcctatt
      421 aaaacatcat cttcaactat aacaggagta gcagaaggtg gctctaaaac accagcaaca
      481 acagcaccag cacctaagtg aacattttta ccaagagttc ctctagctcc aataacagca
      541 ttcatatcta ccatagaacc ttcaccaata acagctccta tatttataac agctcccatc
      601 attacaacag catttttttc tatagtaacc atgtctctta ttattgcacc tggctcaatt
      661 cttgcatgtt cacttaaagt attttttaga ggaatagcag aatttcttct atcatattct
      721 acatgagtat ctgttatatc atttccatgt ttatctaata cttccattat atcctcatag
      781 tcacctacaa atatataaga accattattt ccaaatacct taaatttatc agttgattgg
      841 aagtgtaaag agttagtatt tacatatacc ttaacaggtg ttttcttttt aacttctttt
      901 atataccttg ctatttcata agcatcgttt aaatttacca tttgttaatt ctccttgtat
      961 ttcattatat ttcttttatt atatacttat ttgtcaattt tagtatatac ttacttctta
     1021 ttatttatat tttat
//
LOCUS       DAEMIK010000316         1030 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEMIK010000316 DAEMIK010000000
VERSION     DAEMIK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.322"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            185..1003
                     /locus_tag="KPK87_003878"
     CDS             185..1003
                     /locus_tag="KPK87_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2539933.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
BASE COUNT          462 a           79 c          168 g          321 t
ORIGIN      
        1 gttaagaggg gatatctttt ttatgatggg aatgggtaaa atatttataa ctgacatcta
       61 actgacattt aagctttaaa atcaaaacca tattgatttt ctaaaataat aactatttga
      121 tttagtaaat aataaagaat atagtaacta tatagacaac aaaaagaaaa ggagtgtata
      181 acccttggaa aaacagtatt ttacaacagg tgaatttgct aaattgtgtg gtatatctaa
      241 gcagacatta attttctatg acaaaatagg tattttttct cctgaatata aagataaaaa
      301 taattataga tattactcag tttatcaata tgatacactt gatatattac aatcattaag
      361 agaaatagga atgagtttag aggaaataaa agagtatata caaaatagaa ctccccagct
      421 ttgtgtgaaa atgcttaaag aagaagaaaa aaagataaga gaaaaaataa aaaaactaag
      481 aaagataagt tctaagatgc aaaatagaat aaatataaca gttgaaggaa tttctaaaat
      541 gaatagtgaa gaaatttata tatccaaaaa gtttgaagaa tatttagttc tatcaagtga
      601 tttgagtaat atgagtaata gtgattttat gatggaatta atagatttta ccaattattg
      661 taaatcgaaa aatttgtatc aaggatatga gttgggagta atagtaagta atgaaaatat
      721 aaaaaatggg gactatttaa gtatatcaag tttttatttg aagatagata aaaaaataaa
      781 ggataaaaag ctatatgtaa agccagaagg aatgtatgct tgcataaaac atattggaaa
      841 atatgaggat tcatataaat cttacgaaaa attgaaaaaa tatatatatg aaaatgaata
      901 taaaattgtt ggaaattcgt atgaggagag tatacttgac tttttttgtg aaagtaatga
      961 agataattat atgactgaaa tttcaatcca agtatccaga taagaattaa aatagagata
     1021 tattcaaatg
//
LOCUS       DAEMIK010000317         1029 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEMIK010000317 DAEMIK010000000
VERSION     DAEMIK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.323"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(260..970)
                     /locus_tag="KPK87_003879"
     CDS             complement(260..970)
                     /locus_tag="KPK87_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2539934.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFIAVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          367 a          138 c          118 g          406 t
ORIGIN      
        1 ttaaatttga aaaaaactct aaaaactaag cttcaaaaaa ttgaattttg aaagattgaa
       61 attctagaaa ctaagcttta aaaaattaag tttgaaaaaa caaaactcta aaaactaagc
      121 ttcaaaaaat taaattttga aagattgaaa ttctagaaat taatcttcaa aaaattaaat
      181 ttgaaaaaac aaaactctaa aaactaagct ttaaaaaatt aagtttcaaa aataaaattc
      241 tagagattga cttcaaaaat taagattcta acatatacct tgtaaatttt cctacaacct
      301 cttgatattt tttacggctt ataggaattt tttcattctg aactttggca taagtttgag
      361 ttatactatc tacatggttt agattgacaa tatacctttg atgcgtacgt ataaaattag
      421 agggaagaac agtttccata tcatttaatt ttccgtagta ttctaatgtt ttatcaaagg
      481 taactacagt taattttctt agctcgcttt taaagtagag gatatctttt gtattaattt
      541 tacaaagatt agttccaatc tgaactgcaa taaagttttc tttccattgt tctaattttt
      601 ttagtgcttc ttttaatact ttacatattt tttcagtttt ataaggtttt aagatgtagt
      661 tcaaagctcc tacatcatat ccatgaaata catagtcaga aaaacctgtg acaaagatga
      721 ttatagtatc ttttgaatgt tttcgtatag tttttgctat gtcaacgcca ttactttttc
      781 caagttcaat atctaaaaaa ataatatcta tgtaataatt ttcgtgttct agattgtata
      841 ctagactttc tccatttgaa tattctttta tttggtaggg gtagccccac aaatccattt
      901 ctcttgtaat gattttattc agcatgtttc tttgttgttt ttcatcatca caaatacaaa
      961 tcgaagtcaa ttttttatct cctttcaagt tttatattta tatagttaag tatataatat
     1021 attttgcta
//
LOCUS       DAEMIK010000318         1017 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEMIK010000318 DAEMIK010000000
VERSION     DAEMIK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.324"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(532..714)
                     /locus_tag="KPK87_003880"
     CDS             complement(532..714)
                     /locus_tag="KPK87_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539935.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          423 a          107 c          125 g          362 t
ORIGIN      
        1 ttatagcaaa aaatgaataa atatgaagca agcataattt tataccacaa caattattaa
       61 ttctaatagc tgcacttaat gttttaggta tgggatttaa aaagtataaa aaattaagtg
      121 ataaatatat tccattatat tgcttgtgct tggagtagta ttttctgtat agatgttagg
      181 atttaatcca acttctattt tacaggggat tctatgctgg ggtgtttcaa ttggattaaa
      241 ttaagtatgc aaacaatttc aagacaatga taaataattg gatgtgtgat aggaatatca
      301 acaaaaaaca ttattaaaaa gaattatttt atttataaaa taaaacaagt aataagaata
      361 aataaggagc aactaaaatg atttaaatat actagaatta aaaagagaat gtaaagttgt
      421 ctagtaatgg atgtaaaata ttttaaacga taagctatga aattgatata ttaaaatggg
      481 taagaagaat attatatagg attcttctta cccattttaa taaatttaat tttatgccaa
      541 accacattcg catagtagaa tcatcactct atggagacat aactctacaa catcttctcc
      601 atatttttta ataaaatcac caatttgagt ttctatgaat gaatagtatt cttttaaact
      661 agcaatatat gcagtcatta tatgaacaca ttcatcaatc ttatctttaa acacaactaa
      721 tccccctaaa aaacgaaaaa taaaaatatt cagtaaatag cagatattat ttcactcata
      781 aatctatata gtatgatata ttgcttagat atattttaca agtggatatg taaaactggt
      841 tattataatc gcaaaactgg ataaactttc attcttaaaa gaaaatttat ccagtttaaa
      901 gtttaattat gaatatttga gttagtagat atttaatata aattaatata agtgtaataa
      961 gaatatactt atataattat aattaatcat acttttaagg aatttaattt taaaatt
//
LOCUS       DAEMIK010000319          998 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEMIK010000319 DAEMIK010000000
VERSION     DAEMIK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.325"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..906
                     /locus_tag="KPK87_003881"
     CDS             <1..906
                     /locus_tag="KPK87_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2539936.1"
                     /translation="VSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFK
                     TQKDKLVSQAKKIASPSKGKTELEEIRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVM
                     AIFTNRLGASKKGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPM
                     AKVYSANCYTPEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKC
                     AITLEEFQQANEIHCHHIVPTRAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALL
                     KLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          401 a          156 c          187 g          254 t
ORIGIN      
        1 gtatcacaag aaaaaacaag aattgtaaat gttagaaaaa gatactccga ctttcttggt
       61 ttcaagatta aaatgatacc gagaagaaag aagttagttg ttaaatcaca tataagtgat
      121 aaacaattca agacccaaaa agacaagctg gtatcacagg caaagaaaat tgcttctccc
      181 tcaaagggaa aaacagaatt agaggaaata agactttata attcaatggt attaggtatg
      241 caaaattatt atcagattgc aacaaatgta aatctggatt gtatgaaact taatagagct
      301 gtaatggcta tcttcactaa cagattaggt gcaagcaaaa agggcagatt gagaaaaaca
      361 ggcagacaac ttacaacaaa agaagcaaca agatatggta aatctaaaat gcttcgatat
      421 gttgctggcg agggcgaacc tatattccct gttggatata tccaacacag aaaaccaatg
      481 gcaaaagtgt attcagcaaa ctgctatact ccagagggaa gaaaatttat ccataccaat
      541 ctaagtgttg ataaattact gatgtatcag ttgatgaaat tatctttgga aaatcgcaca
      601 atagaatacg cagacaatcg aatttcgcta ttttctgccc aacatggaaa atgtgctata
      661 actctggaag aatttcaaca agctaatgaa atacactgtc atcatattgt accaactagg
      721 gctggtggta atgacgatta taaaaatctt attttggtaa aagaggcggt acatcgtcta
      781 atacacgcaa aaacagaaga aacaatacaa aaatatatag ctctattgaa actaaataaa
      841 gtccagctca taagactaaa taaatataga gtattagctg gcaatcaaga attaaacttg
      901 atataggtct actacataaa tacttttcaa tataaacacg atggaacgcg gagtgcggtg
      961 aaagtcgcac gctccgtgtg aatgggggga aaagtttg
//
LOCUS       DAEMIK010000320          990 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEMIK010000320 DAEMIK010000000
VERSION     DAEMIK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.326"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            178..684
                     /locus_tag="KPK87_003882"
     CDS             178..684
                     /locus_tag="KPK87_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG2539937.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          394 a           93 c          154 g          349 t
ORIGIN      
        1 tctatatttt acacttataa aatatgaaaa ctacttattg cgaaagtaat tgatttgttg
       61 tattataatt tacatgaatt atgtaggaca atcaaaagtc agtattataa gagtacatat
      121 attcaaattt ttaacaatta atttatataa ttaaaaaatg aagggtagga tgaagtcatg
      181 ggttttagag aagtaaaaat tgaggaatta cagtttaatc catttactaa gattggcaaa
      241 gaatggttac ttataacagc tggtgattca gaaaagttta acacaatgac tgctagctgg
      301 ggtggtgttg gagtctattg gggtaaaaat gttgttacaa cttacataag accacagaga
      361 tatacaaaag aatttgtaga tagtaatgat acatttacag ttgccttctt tgatgaaacg
      421 tatagagagg ctttaaatat ttgtggtact atttctggta gagatataaa taaaatagaa
      481 aaagcaggac ttacaccata ttttgtggat gatacagtag cttttgaaga agctaatatg
      541 attatagtat gtaaaaagct atatcatggt aatatgccac cagaaaactt tgatgctaaa
      601 gaaaatgata aaaaatggta tccagagaaa gactaccata ctatgtacat ttcagaaatt
      661 ataaaagttc ttgtaaaaga ataattataa taaaaaatat atgaaaagaa cttattataa
      721 gtaaatgact gtgctttctt atctgatatc taagtagatg ctagtaacta aataaatatt
      781 aattttaaat aaatactatt ataaaaaaga tgaactatat ttactatagt tcatcttttt
      841 atttatataa gcacttatat aagtagagat ttatttgaat aaaagtattt atttgaaata
      901 tttgagtata ctttataagt atttttatgc taactagaaa cacttaattt atggcatttg
      961 tacttgattt attttaatgt aaataatttc
//
LOCUS       DAEMIK010000321          984 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEMIK010000321 DAEMIK010000000
VERSION     DAEMIK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.327"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            188..328
                     /locus_tag="KPK87_003883"
     CDS             188..328
                     /locus_tag="KPK87_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539938.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(325..696)
                     /locus_tag="KPK87_003884"
     CDS             complement(325..696)
                     /locus_tag="KPK87_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539939.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          374 a          103 c          121 g          386 t
ORIGIN      
        1 tacgagtttt ttattgcaac tgtagaataa aagtattatt atataattaa gataattatc
       61 tagggaaatt ttggataatc aaaatatatt ttataaaatc aaaatacaat tacataaact
      121 ttattaaaaa tttgttggga atttctgact aacattaaga acgtaagttt gttgggggat
      181 ggtatatgtg gaaaagtcaa acactactgt tacaaaaaaa gaaaaaattg aagatatagg
      241 aatgaaatta gaacatttat tgagtgaaga tattactttt tatgaattat tacttgagat
      301 tttgtataaa gaaaaagaag agaattaatt ctcttcttta aggaatcttt ttaaataggc
      361 attatcaatc attttttcta tagcttctct atcttctttt tcaagagaat atattttttc
      421 taagagcttt tttgtttttt cacttactga taagttctct atgattaatt tatcttcttt
      481 gcttattttt gtattatcat ttacgcctaa taaataatct gttgatgttt ctaatgcttt
      541 tgctaatcta actattattt cacttttagg ttctcttaaa ttattttcat aacgtgacaa
      601 gctagcctca gttataccga ctttagtagc aagttctttt tgatttatat ctaattcttt
      661 tcgtagattt gctattctat ctcctaaact ttccatatgt acatcactcc ttaccgaaat
      721 tataagttat tttgataaaa agtaaaataa aattaccaaa aatataaaaa aaaaaggtta
      781 aaataataaa atatattgaa aattaccatt tgtatatgta taatatgaat tatacaaatg
      841 gtaatttgat gagaggtggt atgttgaaat aagaaactga attaattaat aaatacatag
      901 aaagttttga aaacctttaa ttgagatata aacaatatgt acaaaatttt ttgcaagtaa
      961 aattaccaaa tgtataaaat aaat
//
LOCUS       DAEMIK010000322          975 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEMIK010000322 DAEMIK010000000
VERSION     DAEMIK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.328"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            281..>975
                     /locus_tag="KPK87_003885"
     CDS             281..>975
                     /locus_tag="KPK87_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2539940.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGTPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMPDGTITYP"
BASE COUNT          382 a          158 c          196 g          239 t
ORIGIN      
        1 agcaggctta tagctagtga cctaaggata tatatgtata gataagatta tcggaacgag
       61 gaaaggtatt gggttctcat caagagaata agctgacgaa gaacaataag cagttgaacc
      121 aatgctgaaa agcagagggc gaacctaaga aattcttgta atgagaatgg aggaataccc
      181 tcaagtcgtg tatatattag aaaagtatta tgttgtttta ataaggatta cgagtaagat
      241 aaaaaagctc actcaatttc aagcaaagga gtgaagcctt atgcctaaaa ataaaaatga
      301 aacattgtgt gtagaagatt taagacacgc tgaatattac gaaatgcaaa acacctttga
      361 tgatttatat gccaaaagta agaatggaga tattttcact catcttatgg atattatcct
      421 atcgagagaa aatatattac tggcatatcg aaatatcaaa gcaaatgcag gcagtaagac
      481 agcaggaaca gacggtacaa taattaaaga tattggcaaa ttgccagcag aaacagttgt
      541 caaaaaggta agatatattg tcgcaggcac tccgcacggg tatcgaccta agccagtaag
      601 gcgaaaagaa ataccaaaac caaatgggaa aaccagacct ctaggcattc cttgtatgtg
      661 ggacaggctt atacaacaat gtatcaaaca agtattagaa ccaatctgcg aagctaaatt
      721 tagtgaaaac agctacggtt tcagacctaa taggtcggtt gaaaatgcaa taaaagcaac
      781 ctataaccgc ctacaaatca gccagctaca ttatgtgatt gaatttgata ttaagggttt
      841 ctttgacaac gtgaaccatt ccaaactgat aaaacagata tgggctatgg gaatacggga
      901 caagcacttg atattcattt taaaaaggat attaaaagct ccaattaaaa tgcctgacgg
      961 cacaataaca tatcc
//
LOCUS       DAEMIK010000323          951 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEMIK010000323 DAEMIK010000000
VERSION     DAEMIK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.329"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..>951
                     /locus_tag="KPK87_003886"
     CDS             27..>951
                     /locus_tag="KPK87_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2539941.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIFI
                     IESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNFKIGILLFFYFLFSQI
                     IFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGI
                     VMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIM
                     HVLINSLGLIFNDSEIIINYNSIIQRLIFILL"
BASE COUNT          327 a           86 c          119 g          419 t
ORIGIN      
        1 tttataaaaa agggaggtta tgtttgttga atttacaatc aaaatgggta aactttttaa
       61 agccaacata tgtcagtgat gaaataaaaa gaaaaaaact acaagttata atattttttt
      121 ctataactta tggtttatct tattcactag gtttattagc aatatgtttt aatcatttaa
      181 tagattcaga aattttagct caatttatga tgatattgcc attatcttca gtttctattg
      241 caaaatttta cacagaggga agaaataatg ataaatataa tttttattca tcagtaatat
      301 tattttttat tatttacttt tttatattta ttattgaatc attaaagtta ataagtatta
      361 atcaatttca attaatgtat cttatgttaa ttttaataag tagtttatgt gttattgttt
      421 attcttataa aataacaagt ttatatattt ttaaaaattt taagataggt attttgctat
      481 ttttttactt tttattttct caaattattt ttggattaat aataagtgat aatcaatttg
      541 attataagaa tttcttgaat tatattcttc ttcctataat atcattagta tacatttatc
      601 cttttttatg tgaagagtat ggttggagat cttttttgca aagtatttta tttgatagat
      661 ttggcaaaaa aataggtatt gtaatggtag gcacttgttg gagtttatgg cacttacctt
      721 tacaatttac actatatagt cctcaaactc caataattgg agctatagcc catttgatat
      781 atggtattgg actttctata tttttaggtt atgtttatat gaaaactaag aacatatggt
      841 tttgctctat aatgcatgtt ctaattaata gcttgggatt aattttcaat gattctgaaa
      901 taattattaa ttacaatagt attatacaaa gattgatatt tatactatta t
//
LOCUS       DAEMIK010000324          950 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEMIK010000324 DAEMIK010000000
VERSION     DAEMIK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.330"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            42..911
                     /locus_tag="KPK87_003887"
                     /pseudo
     CDS             42..911
                     /locus_tag="KPK87_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
BASE COUNT          376 a           82 c          173 g          319 t
ORIGIN      
        1 cttagaaaat ataatatagg aaaattgtat ggtaaataaa tatgaaaaat agttttaaaa
       61 attgtattag tttaagaaat aaatttataa ttatatcaat tgtgtttcta ttaatcctat
      121 taattgtatc ttatatattt ggcaaattag tgtataatag ttcagttggt ttagagcagt
      181 taataaaaaa cgaagatata gtaaaaatat tttctgagag aaaagataaa ccgctggaga
      241 aattaaaaaa ctacaagaca tctgaattaa tgatacaaag ttctaataat tacaagcttg
      301 aaagtctttt tataacttca aatataaaga caagagatac tatgatactt gttcatggaa
      361 ttggaagtag ttactatgaa atgttaaagg tagcatatag atatttagat aaaggttata
      421 atgtattagt ttataatcaa agaaatactg gtaatagtgg tggagataat tacacatttg
      481 gtctttatga aaggtatgat ttagatagtc tagttaaatt tgtaaaaaat aaatttcctg
      541 aagggagatt aggtgtgcat ggattttcca tgggggcagg gacagctgct atgcattcag
      601 agataaattc aaaagatgat aaggtcgatt tttatatact ggattctcct tattctgaaa
      661 tgaaagatgc aattagaatg ggagtactag aaaaacgaat accagatata ttaatcaatt
      721 atgtggtgac ttgtggagat ttatataata agtttaaatc tggattttgg tacagtgatg
      781 taaaaccata tgagtcagtt gagaaatcta atgtacccat actgtttata catggaacaa
      841 aagatactgt ttgtaattat caaaatagca agaaaatgta tgatttagtt aaacatgata
      901 aaaaagacct atgacttata gaaggtattg gtcatgttga tggatttgaa
//
LOCUS       DAEMIK010000325          950 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEMIK010000325 DAEMIK010000000
VERSION     DAEMIK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.331"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..538)
                     /locus_tag="KPK87_003888"
     CDS             complement(<1..538)
                     /locus_tag="KPK87_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539942.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMV"
     gene            complement(549..>950)
                     /locus_tag="KPK87_003889"
     CDS             complement(549..>950)
                     /locus_tag="KPK87_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539943.1"
                     /translation="TVVLTISLFIASKLLPRHMLGNVTNYGFVQETIYELKMLLPYSF
                     EILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISFLFLVIEILVFIISIEFIIPYE
                     YSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
BASE COUNT          348 a          143 c           75 g          384 t
ORIGIN      
        1 ttaccatata taaattacct ttatatattt tgttaaataa ggttttccaa ctattttctt
       61 taaaatcaga cttatttaaa ttaatatcaa aattttcttc taccccactt tttaaatttt
      121 tatttgaaac ctttatacaa tcatttaaag cattcattcc ttgtactaaa tcagacttaa
      181 cagaggtata cgcttctcct atattcaaat catctttata tataaattct tttcctttat
      241 cattaacaaa gtcactgtat tttttgtcaa tatttaaata atcatttcta attcttgatt
      301 ttgggtttgt attatttctt actccatttt tacttataat tacctcctca taagaaaatt
      361 tgcttatatt aggtgtatat actacactta ctctccctat atcttttaca ttgcctttta
      421 aagtttttac agatagttta tccttattaa aataactcaa tattaaggtc atagtaatca
      481 ttaaaaaaca tactattata aaacatattg tatattttcc tatatttttt gctttcataa
      541 tatccctcct aaaagtcaat cttttgcttc aaaagtttat tagatataag actacataca
      601 aatatgttta tacttgaata tagaacagta aatacatcac tgtattcgta aggtataata
      661 aattctatag aaattataaa cactaaaatt tctataacta aaaatagaaa acttattaaa
      721 gtgcttactt tataaataga tttatttata agaattgatg taaatactac agatacaaat
      781 cctatcaata aaaatatata ctctacaaat agtatttcaa agctatatgg aagtaacatt
      841 tttaactcat atattgtttc ttgtacaaaa ccatagtttg tgacattacc aagcatatgt
      901 ctaggcaaca acttacttgc aataaacaaa gatattgtaa gtactactgt
//
LOCUS       DAEMIK010000326          930 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEMIK010000326 DAEMIK010000000
VERSION     DAEMIK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.332"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..833
                     /locus_tag="KPK87_003890"
     CDS             <1..833
                     /locus_tag="KPK87_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2539944.1"
                     /translation="YLIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKD
                     IKLKYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKT
                     VYWFNPFIYIMDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFI
                     AGLHKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGME
                     EKDVTNSAIVTMIEFTYAEAPKSMRERYKINCEFLGLIPKDSDKIGVGVEYYDSLLND
                     "
BASE COUNT          377 a           82 c          137 g          334 t
ORIGIN      
        1 tttatttaat ttacactata tttaaatata taaaacttaa aaatttaata aatgacttat
       61 cttatgatgt tgatgatgga gagattgtaa atctttataa aaatatatta gaagaattta
      121 atataacaaa agatataaaa ctcaaatact cttatgaagt tgaaacacca gcatttttta
      181 attcatgtgt gcttttgcca ccacatgagt ataaattgaa agagttagac tggattttta
      241 gacatgagct tatgcatttt aagagtaggg acctttattt aaagtatgta atcctgtttt
      301 taaaaacagt atattggttt aatccattta tatatataat ggataaacat atagatttag
      361 attgtgaact ttattgtgat gaaagagttt taaaaaatcg aaattcagag gaaaaaaagg
      421 attatgcttt aacaattcta aatgcaatga gaaaaggttc aaatacttca aataaattta
      481 tagctggact tcataagcaa tctgatatta aaaagagagt tatgaacatg tttaatgaaa
      541 agtacaagaa tggtatatta atggcattaa ctttatgttt attatcttct attacttttt
      601 tgaaggttaa ttcaactagt attgttaatc ttaatgtatt gccaaaagga atggaagaaa
      661 aagatgttac taatagtgca attgttacaa tgatagaatt tacatatgca gaagctccta
      721 aaagtatgag agaaagatat aaaataaatt gtgaattctt aggtttaata cctaaagact
      781 ctgataaaat aggtgttggt gtagaatatt atgacagtct attgaatgat taaataaata
      841 ttattttaac agatattaat tataaagttt tgaatagtaa tttgttgaga aataaatata
      901 tccttataaa aatagatact tatataccaa
//
LOCUS       DAEMIK010000327          908 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEMIK010000327 DAEMIK010000000
VERSION     DAEMIK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.333"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(312..779)
                     /locus_tag="KPK87_003891"
     CDS             complement(312..779)
                     /locus_tag="KPK87_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2539945.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDIGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          355 a          158 c           57 g          338 t
ORIGIN      
        1 atttgttcaa ttaaatttta acataattac ataaaatagt atatatgatt ctacagcttg
       61 atagtaagta taattatctt ttataagcta atacttatat ttttctaaat ttaaatcatc
      121 caataaatat taaattctat tataatatgt aaggaaaatc atgcactttt taaaaatcca
      181 aataaaatta taacaaatat tatattctgt atcttacaaa aacaaaaact gtaagtaaat
      241 ttgtttccat acaagtttac ttacagccct tacttctata caaaatatag ttttacttta
      301 atataatcca tctattctaa aactgattca atatattcga cagttacatc cattctttta
      361 gctacctcat ctattgaaag accaatatct aaaaaacgtc tacacacaga tgacatagtt
      421 tctccaacct caataatatg cccatctaaa tcataaaatc taattgctct ttgaccccac
      481 ctatgctcta tagctagatg aacataatct atatctttca ttgtactcaa tctttcaaca
      541 aaagtatcaa aattatcttc ctcaaaatat aactcagcat catttccatt gaacttaact
      601 tcactgtcac tcttattaat aaattctaac caaagctttt tttcttgtaa tgatatatta
      661 ccttctaata caacattttc tccaaaatca cagattacct ttaaccccaa aacatcataa
      721 taaaactttt ttgatttctc aatatcttca actactatta aaacatttaa aaacttcata
      781 ttacctcctt aatatacaca tactttattt ataatatata attagaatta aactataaag
      841 gtttcaataa ggtctagtat aaagaaaaaa attataagtc ttattgaaat agttaagtta
      901 attttatc
//
LOCUS       DAEMIK010000328          894 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEMIK010000328 DAEMIK010000000
VERSION     DAEMIK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.334"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            121..498
                     /locus_tag="KPK87_003892"
     CDS             121..498
                     /locus_tag="KPK87_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2539946.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          356 a           64 c          133 g          341 t
ORIGIN      
        1 aaatataaat tttttgtatt aataaatttg gatgagatat tttaaataaa acctttgact
       61 cgaaaagtct attaatatag tatttaatta tgtagaagtt tgagaggagt gtaaaatcat
      121 atgactatta ctaagatacc accagcagaa ttaaaagtca tgaaatttat ttggagttca
      181 gattttacag tagcatcaaa agatgtcatt gaagctatgg aaaaactata taattggaag
      241 caaactacaa cactaacact tttatcaaga ttagtaaata agcaattctt aagtgcaaga
      301 aaaattgata gacatacaca ttatacagtt cttattggag aagaagagta tttacatttt
      361 gaaacaaaaa cttttttaga taatatgcat ggaagttcta ttgagagttt tatgaaatcc
      421 ttgttcaaaa agggggtaaa tgaagaagaa ttgaactctg ttgaaaaatg ggttaaagat
      481 attaaaaaag atgaataatt tggtgtagtt aattatcata aaataaatag tttttacaat
      541 aattagttaa atgaatcaaa tataaagttt tatgtaaaat ttgtgatttt tataaatatt
      601 tttgtcttgt tatttaatat taatacaata agtttgatgt aaaatcaaac ctgtttgaat
      661 ttaatttata ttgggtttgt cattaatatt attttagttt gatattgtat acgaatttaa
      721 aagtcagaat ttttgtatat taatcatgta aattaattgt tttagatata aagggcataa
      781 ttttattttt gttttgatta tttctagggt aagataagta tacaatagat tgtattaaac
      841 atgcttatat tgatagattt gagataaata ttgagatata ttaataatta ttaa
//
LOCUS       DAEMIK010000329          881 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEMIK010000329 DAEMIK010000000
VERSION     DAEMIK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.335"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..384
                     /locus_tag="KPK87_003893"
     CDS             <1..384
                     /locus_tag="KPK87_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG2539947.1"
                     /translation="DSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSVYV
                     LINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKIMN
                     KNKGNKEGDSNESNRFDTESYGNNS"
     gene            338..766
                     /gene="ytfJ"
                     /locus_tag="KPK87_003894"
     CDS             338..766
                     /gene="ytfJ"
                     /locus_tag="KPK87_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG2539948.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
BASE COUNT          365 a           78 c          154 g          284 t
ORIGIN      
        1 gattcaagta tattattagc tgatgacctt ttgagcattt atagattatt aaaaaagata
       61 aaaatacatg aactttattc aaatattaat tttggaactg gaaatattgg cttaacatct
      121 tctgtatatg tattgataaa tactttgtat ggaaatttat ttaatatgat tgatgcagaa
      181 aaaatgtatt taaatgtaaa tccagatttt acaaaagatt atgttttagg aaatataagg
      241 atacatataa ggccaagaat aaaagcttta tttaatatta taataatgat taataaaatt
      301 atgaataaaa ataaaggaaa taaggaaggt gatagtaatg agagcaacag gttcgataca
      361 gagtcttatg gaaacaactc ttgaaacaat taaaggttct atagatgcaa atactattat
      421 aggagaccct ataaaaacag atactacagt ggttgtacct atatcaaagg ttacaattgg
      481 ctttggtata ggaggaggag aatactcaaa aggatatgat gataaagata gggaaattga
      541 attaaaaaat gaaaaatcag atacgaattt tgctggaggt agcgcagggg ctatatctgt
      601 acaaccagta gcatttgtag tagttgaaag tggagagact agaataatga gtttagacag
      661 taatatcaat ctagtggata atatattatc tataactcct agagtattgg agaaaattca
      721 aaatatatca caaagtaata agaatcaaga taaaaacaat atgtaagaat aaaatatttt
      781 taaatatgta tatagtttta ggtaaagtag tggggttagt ataaatccca ctattttgtt
      841 gtgtacttta aacatttata tctaataaga ttttagttta a
//
LOCUS       DAEMIK010000330          833 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEMIK010000330 DAEMIK010000000
VERSION     DAEMIK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.336"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..576
                     /locus_tag="KPK87_003895"
     CDS             106..576
                     /locus_tag="KPK87_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG2539949.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          384 a           58 c          136 g          255 t
ORIGIN      
        1 aaaaagtaaa aataaaggat taataaaagg tactgaagag atagagaaaa aaatgttaat
       61 agaagatact gaaaaaatag aaataaaaaa ttaggaggaa aatttatgaa atatcaggtt
      121 tcattattag cagtgaagga tattgaagta tcaaaaaaat tttacaaaga attatttgag
      181 caagaaattg aattggactt aggtaaaaat gtaacattta aaggaggatt tgctatacaa
      241 gaagacttcg catggcttac agggattaat ccagatacaa ttatacaaaa atcaaataat
      301 atggaacttt attttgaggt tgatgatttt gacgaattta ttgaaaaact caattattat
      361 aaagatgtag agtttgtcca taagccacta aaacatgaat ggcaacaaag agtagtgcgt
      421 atttatgacc cagataagca tattattgag ataggagagt ctatggaagt tatagctaga
      481 agatatttat ctgagggata ttctgtagaa gaaacttcta aaataattca acatccaatt
      541 gaatttgtaa agcagtgtga aaatagtaag gaatgattgt ttaaattata attatttagt
      601 agaaattaat tgtagtagaa ctaattcaag ataatatata ggactagtat ttgacaaatg
      661 tcataaatta aattaaaata aagaaaatat taataaagaa aaaataaata aaaataaggg
      721 gaatattaat aaagaaaaaa taaatataat attaaaatat attaataaat ttgaatattt
      781 ataatatttt taatttgaag taggaatact taaacgtata aagtttatta tcg
//
LOCUS       DAEMIK010000331          829 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEMIK010000331 DAEMIK010000000
VERSION     DAEMIK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.337"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..773)
                     /locus_tag="KPK87_003896"
     CDS             complement(<1..773)
                     /locus_tag="KPK87_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG2539950.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYT"
BASE COUNT          330 a          149 c           79 g          271 t
ORIGIN      
        1 atagtataag atttttcttt atctaatagt atatcctcta catctgggtt ttcttttact
       61 aattgtctat aaaaattaca attagtaaca cctacatatg gaattagcca tagtagacct
      121 attccaagag tagctattga aagtatatac catcctataa aactaaagtt tagtacaaat
      181 aatttaagta catttccttt catcatatct aaactagcac ccattgattt aaatacaccc
      241 atattatcct tgtcttcaca aattaagaaa gtagcttgag aaacatataa tagcaatata
      301 taaaccggta acataagaac aagtattata agaactagag ttattccagt agaaataaca
      361 gtccctattc caacattatc acttgataaa tattcaattg ctccactagt aagtaaaact
      421 ccaaatgata aaaacgctcc tataattgtg attataaaag aataaatata aacaataatt
      481 gattcaaata aataaaaacc aaaacttctt aaataaattc tcccacttac aaagatatct
      541 cctatcttga ctttttcatc tttagtaatt ttcaagaaca tacatgtaaa tcctgctgaa
      601 acgcaaaaag ctattacagc cactacaata gtagtcaatg gttcaaaagg taatcctaca
      661 attgatatta atactatcaa tgcaatatat actgccatag aaattgcaca tatcccccag
      721 tttccagata actgagcttt agatactcgt tttaattctt gccttgatac cataaaaaac
      781 acccctttca ttttataaaa aaacttttaa aaatataaat aatctttta
//
LOCUS       DAEMIK010000332          803 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEMIK010000332 DAEMIK010000000
VERSION     DAEMIK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.338"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            297..740
                     /locus_tag="KPK87_003897"
     CDS             297..740
                     /locus_tag="KPK87_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539951.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT          317 a           67 c          124 g          295 t
ORIGIN      
        1 tagtattata gatgaatatt agtattgata ttctaggtaa ctgctagagc taatattata
       61 gatgaatatt agtattgata ttctaggtaa ctgctagagc taatattgta gatgaatatt
      121 agtattgata ttctaggtaa ttgctagaga tgatattata atttattatt aatattaata
      181 ttctagggaa ttatcactat attttaaaaa gtaaaaatat atatcaaaat gaactaaata
      241 agtactgtaa gaagtttgtt aaattaaggt ttgaataaag tgagggtgga gaacttatga
      301 tagaaataaa aactatagaa ataaataaaa aaagtcaaat tgaccttttg atattagctg
      361 acccatgtga agaaatgatt gataaatatc tcaataaagg aactatgtac gcattgtatg
      421 ataataaaga acttacttgt atagcagttg taaatgaaat ttccaaagaa atatgtgaat
      481 taaaaaacat tgctacatat gaacattttc aaaatatggg ttatgcctca aagatgattt
      541 atcatctttt agatgtttat agtaaaaaat attcatcaat ggttgttggt acatctgaaa
      601 gcggagttcc tttttatgag aaatttggat ttgtttattc acataaaatt aaaaactttt
      661 ttgtagataa ttatccagaa ccaatctttg aaggagaatt acaatgtgtt gacatgttgt
      721 atctatcata taacttttag ataggataat atttttaggt taatataatt aattaattca
      781 tataattatc ggaattataa ttt
//
LOCUS       DAEMIK010000333          802 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEMIK010000333 DAEMIK010000000
VERSION     DAEMIK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.339"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>802
                     /locus_tag="KPK87_003898"
     CDS             <1..>802
                     /locus_tag="KPK87_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG2539952.1"
                     /translation="ILSAPKNLKLLKETLDIDEITFNDCLKFDEITKLDLFDNYDFLS
                     LNTFELRDGEAVIEEVNMYLSDNFILVVVNEEHFLFEFVKNIILKNSQLEKNPVINLF
                     KINYLILREVIKNGFESLEKVEELILQIEDEMMDNINKNHVSRISDVRGLTRIIVKNT
                     RPLLYIGDRIVKENIRYLKYSNVKKYNLENFQGIDFGIDKLYNFALSTRELADKLLDI
                     YSSRVGEKTNNLITKLTLLTAISAPLTIITGIYGMNFRYMPELNWIYG"
BASE COUNT          328 a           74 c          125 g          275 t
ORIGIN      
        1 ttatattaag tgctccaaaa aatttaaaat tgcttaagga aactttggat atagatgaaa
       61 taacttttaa tgattgtctt aaatttgatg aaataaccaa acttgattta tttgataatt
      121 acgatttttt aagcctaaat acatttgaac taagagatgg agaagctgtt atagaagagg
      181 taaatatgta tttatcagat aattttatat tggttgtagt gaatgaagaa cactttttat
      241 ttgaatttgt aaaaaatatt attttgaaga actctcaact tgaaaaaaat ccagttatta
      301 atctatttaa gataaactac ttgattctta gagaagttat aaaaaatggt tttgagtctt
      361 tggaaaaagt tgaagagtta attcttcaaa tagaagatga aatgatggac aacattaata
      421 agaatcatgt atctagaata agtgatgtaa gaggattaac aagaattata gttaagaata
      481 ctagacctct tttgtatatt ggggatagaa tagttaagga aaatataaga tatttaaaat
      541 attcaaatgt aaaaaaatat aatttagaaa attttcaagg catagatttt ggaattgaca
      601 aattatacaa tttcgcattg tcaacacgag agttagcaga taaattatta gatatttatt
      661 cttccagagt aggtgagaaa acaaataact taattacaaa attaaccttg ttaacagcca
      721 tatcagcacc acttacaatt ataactggta tatatggaat gaactttaga tatatgccag
      781 aattaaactg gatttatgga ta
//
LOCUS       DAEMIK010000334          786 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEMIK010000334 DAEMIK010000000
VERSION     DAEMIK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.340"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..527
                     /locus_tag="KPK87_003899"
     CDS             105..527
                     /locus_tag="KPK87_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG2539953.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT          358 a           72 c           97 g          259 t
ORIGIN      
        1 agctatataa atagttttaa catgatttaa tatggcatat tatactattt taacttaaaa
       61 attaacttat aaatattaaa tttatataca gaagggaaaa cgatatgaat acatttaaaa
      121 ctgaagtttt attaaaatat tctgcaaaag acatatttaa tatatttaca aaatctgcaa
      181 gactaaattt tcctaatttt aatgagaaaa aagcagtagg aacatttgta cagcaaaaaa
      241 ataataaaag atttaaggtt gaaataacta atttcgaaaa aaatagagta tatgagataa
      301 aaacaagcaa taaaagagaa agtactatta ctagatatga attagtacat attgacttag
      361 aaaatacaaa acttattttt attgaaagtg agagcaagag gaatatattt ggaaaaatca
      421 attcagctct tgtaagagta ttgtttggaa aaaggcaacc agaagaattt aataaattta
      481 taaaaaaact agaaatggaa ttggagaata atagtgttaa tttatagata taagttagat
      541 tatatattta aaatctatct aaaaacatat ttctaagtat tttatgagtc ctactattcg
      601 ttacaataat taatgaaaaa actattgata aacatgcgac actatgacta tataccacat
      661 taagagtatc attagcaata ataattttat gtattattgc ttttagttaa aaaaagagag
      721 aaaaattaat aatctctaat atcccttaaa ctttcaataa agaggataat ctttatctaa
      781 taagat
//
LOCUS       DAEMIK010000335          766 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEMIK010000335 DAEMIK010000000
VERSION     DAEMIK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.341"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            154..270
                     /gene="rrf"
                     /locus_tag="KPK87_003900"
     rRNA            154..270
                     /gene="rrf"
                     /locus_tag="KPK87_003900"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            276..364
                     /locus_tag="KPK87_003901"
     tRNA            276..364
                     /locus_tag="KPK87_003901"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Leu,seq:taa)
     gene            376..452
                     /locus_tag="KPK87_003902"
     tRNA            376..452
                     /locus_tag="KPK87_003902"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:410..412,aa:Ile,seq:cat)
     gene            562..>766
                     /locus_tag="KPK87_003903"
     rRNA            562..>766
                     /locus_tag="KPK87_003903"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          250 a          140 c          174 g          202 t
ORIGIN      
        1 taactctata tttttaaact tgtcaaaact tatggatttt taatagtttt ataaggcaaa
       61 aaatcgaata aaaaaagtat tgacacaata agacaaagat gttattataa tacttgtctt
      121 agaaaaaaag ttaaatgaaa atttagacaa taatctggtt ataatagcag agaggataca
      181 cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac ttggtgggaa
      241 actgcctggg agagtaggac atagccaggt gatgtgccga agtggcggaa ctggcagacg
      301 cacaggactt aaaatcctgc gggacttacc tctcgtaccg gttcgattcc ggtcttcggc
      361 accacaaatt attgtgggcc attagctcag ttggttagag cgcccggctc ataaccggta
      421 ggtctggggt tcgagtccct gatggcccac cacaataatt taaagaactt tgaaaattaa
      481 acagtaggtt aatttataaa caagaaacaa accataaagc cagatatttt gataacaata
      541 gtatctgagc ctgataaact tttatttgag agtttgatcc tggctcagga tgaacgctgg
      601 cggcgtgcct aacacatgca agttgagcga tttacttcgg taaagagcgg cggacgggtg
      661 agtaacgcgt gggtaaccta ccctgtacac acggataaca taccgaaagg tatgctaata
      721 cgggataata tatttgagag gcatctcttg aatatcaaag gtgagc
//
LOCUS       DAEMIK010000336          743 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEMIK010000336 DAEMIK010000000
VERSION     DAEMIK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.342"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>743
                     /locus_tag="KPK87_003904"
     rRNA            <1..>743
                     /locus_tag="KPK87_003904"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          205 a          153 c          226 g          159 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gat
//
LOCUS       DAEMIK010000337          716 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEMIK010000337 DAEMIK010000000
VERSION     DAEMIK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.343"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            109..477
                     /locus_tag="KPK87_003905"
     CDS             109..477
                     /locus_tag="KPK87_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG2539954.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          304 a           61 c          117 g          234 t
ORIGIN      
        1 atataggttg gattttatag atacaaaaat agtatatatg atatgtagac cattagcatg
       61 tttaaagtag caaaacatat aaaataaaat ttgaaaggaa gtataaaaat gacaagacca
      121 tcaatatatc atagccaagg atacacttgt gcagaggcat taataaagtc ttataatgag
      181 gaacacaaca cagatatacc aatctcaata ggaagtggta tgggggttgg aatgaatgtt
      241 ggaagtgttt gtggagctgt aaatgctgct gttgttataa tagggtattt aaatgggcga
      301 aatagtaatt tagatgaaaa tatagcaagg aagtattcaa gagagctatt gaatagagta
      361 agaaataaat acagctcaga aatatgtgta tctcttaaaa aaaataaagt aacttgtgaa
      421 gaaataataa atttcacata tgatgctcta aatgatatgc taaaaaatca aaaataattc
      481 taagaaacaa gtattttaca ttcgatagaa tacttgtttt tttatgatat ttttaatagg
      541 tattagttta ttataatttt taaatcaatt ttattatatt ttaattgaaa taatctactt
      601 tgaaaattac taactgacaa aagaatatga ttatagtata gaaaaaatgt aaattattta
      661 atattaagtg agagtatatg cttacctata taaaatcgta taaattaatg gctaaa
//
LOCUS       DAEMIK010000338          693 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEMIK010000338 DAEMIK010000000
VERSION     DAEMIK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.344"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            200..568
                     /locus_tag="KPK87_003906"
     CDS             200..568
                     /locus_tag="KPK87_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2539955.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          280 a           72 c           91 g          250 t
ORIGIN      
        1 aatatatgtc atattcttat ctctaataga aaatatattc ccctcttcca taaaactctc
       61 aatatctttt ttatctataa atctatacta cttagttctc aagcataaaa taatataaag
      121 attatttata catgatgata tattatattg actattagac atttgtctaa tataatcaag
      181 ttaaaggtga gtgattatat tgaaaaaaat accaaaatcc gagttgttag ttatgaattt
      241 catatgggat aaaggcatag ataaaatacc agccagagaa gtagctaatt atatggaaat
      301 ttctcatgga tggacaaaag gtactactgg taaagtactt tctagattaa gtgataaagg
      361 ttttttagaa tttgaaaaag atggtagaaa tactttatac actattaaag ttaaacatga
      421 ggaatatcta aaatttgaaa caaaggattt tttagagctt atacataaaa attctattca
      481 tagctttata tcatcattag gttgtggaaa tgaaatatca gatgatgata taaaagaact
      541 tgaacaatgg ctaaaaaaaa gaaaatagga gacgtatttt atggaattat ttatagaagt
      601 tattaaattt aatatttaca taagtccact aattcttttt ttcattttat ttactaaaaa
      661 gataagcaaa tatactttta agtttaatta ttt
//
LOCUS       DAEMIK010000339          689 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEMIK010000339 DAEMIK010000000
VERSION     DAEMIK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.345"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          301 a           54 c           96 g          238 t
ORIGIN      
        1 caattaaacc aaaagactca ttgtatattt gaaattaagt tttttatagt ttataggtgt
       61 tttatgtgga ttacgaatga tatagtatcg atacattatt taatagattc ttatgccaag
      121 aaacaattaa tttatagaaa tccttaattg ttacaatata aatatctaga taagttataa
      181 gatactgata aataatttat aaagtttatt aatctaatat tgtgtgtgaa tattaagtgg
      241 tcaagtagtc taaaaatgaa aaagatttaa aaaagtgttt taactaaaca cataagtgtt
      301 aaattcatgt ataagtatag gttagataca taagaaataa ttatgtaata tctatctttg
      361 taaaaaaata ttaaattttg taatttgtgg ataaatatgt ttataatgtg gataatattt
      421 aatgtaataa gttaaagcaa tatgaatcaa ctgtttataa tgtggataat attataaatg
      481 tatgaataat tgcagtattt aaaaacaaaa aaagttaaaa aagttgttga ctctaaaaga
      541 aaaagttggt atagtaatac ttgtcctcaa gaaaagggca aacaaataaa gcacaaagaa
      601 ctttgaaaat taaacagtag gttaatttat aaaacaagaa acaaaccata aagccagata
      661 ttttgataac aatagtatct gagcctgat
//
LOCUS       DAEMIK010000340          663 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEMIK010000340 DAEMIK010000000
VERSION     DAEMIK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.346"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            275..>663
                     /locus_tag="KPK87_003907"
     CDS             275..>663
                     /locus_tag="KPK87_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG2539956.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFY"
BASE COUNT          284 a           49 c          104 g          226 t
ORIGIN      
        1 tagtttttat tctatattat aattatatta ctaaaaaata atatatggta tataatataa
       61 aaaaagattg ataaataaat aaaaaatata ttatgttatt tatttgaata gattatccag
      121 aagacataaa taagaaaaat aatgtgtaag taaaaatatt agaatttatt aaaagctatt
      181 acaagtataa gaaatttgaa atgattattc aaaaattgac atgtgaaaaa aaatatgata
      241 tgctgcaata aaagttaatg tgaggtatgt cagaatgtat aatgttatag atctttttgc
      301 aggtgcaggt ggtttgagct taggatttga aatgactaaa aaatttaata tggtggcttt
      361 tgtagaaaaa aatgataacg cagcaaaaac ataccttgaa aatcatccga gtgttaagcg
      421 ttattgtgat attaaaagat tagattttca agatatatta aacagtgtcg ataagataga
      481 tgttgttata ggaggaccgc catgtcaagg tttttctaat gctaatagac aaaaaagaaa
      541 aattataaat ggaaataatg aattagttaa gctatatgtt gatgctattg ataaattaaa
      601 gccaaatgta tttgtaatgg aaaatgtcaa aactatatct tcaaataagc attcatttta
      661 ttt
//
LOCUS       DAEMIK010000341          652 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEMIK010000341 DAEMIK010000000
VERSION     DAEMIK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.347"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>652
                     /locus_tag="KPK87_003908"
     rRNA            <1..>652
                     /locus_tag="KPK87_003908"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          169 a          143 c          197 g          143 t
ORIGIN      
        1 actgtcaggt gggcagtttg actggggcgg tcgcctccca aaaggtaacg gaggcgctca
       61 aaggttctct cagtacggtc ggaaatcgta cgtagagtgt aaaggcaaaa gagagcttga
      121 ttgcaagaca tacaggtcga gcaaggatga aaatcggact tagtgatccg gtggttctgc
      181 gtggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      241 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcatca catcctgggg
      301 ctgtagtagg tcccaagggt tgggctgttc gcccattaaa gtggtacgcg agctgggttc
      361 agaacgtcgt gagacagttc ggtccctatc cgtcgcaggc gtaggaaatt tgagaagacc
      421 tgtccttagt acgagaggac cgggatggac gtacctctgg tgtaccagtt gtcctgccaa
      481 gggcatggct gggtagctat gtacggaatg gataagcgct gaaagcatct aagcgcgaag
      541 ccaacttcaa gataagattt cccaccgcaa gggtaagacc ccagaaagac tatctggttg
      601 ataggtcgaa ggtgtaagtg cagcaatgta tttagcttat cgatactaat ag
//
LOCUS       DAEMIK010000342          646 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEMIK010000342 DAEMIK010000000
VERSION     DAEMIK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.348"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            220..501
                     /locus_tag="KPK87_003909"
     CDS             220..501
                     /locus_tag="KPK87_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539957.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          294 a           38 c           88 g          226 t
ORIGIN      
        1 tattttgtta aatttagata caataattgt aaagtaaaat ctcaaaatat acaattatta
       61 taaatcatag atatttatta aataaatata ttaaagaaga aggaatataa aacaagatta
      121 gaaaataata gtgttacttt cataataaat acatataaag atatttttaa gaataaaatt
      181 tattattaaa taaaaaataa aggagagtga tttaatagca tggcatcaaa acaaatacca
      241 agaaacaatt tagaaaagtt tttaagaata agagtcttat cacatacagg cgataaaata
      301 catataaagt tacctgtaaa ttttgcaaag agaatgattg agaataatgc tttggattta
      361 tttaatggaa aagatgatgt agtagatggt aaaaaagtta ctgaaatatt agttaaggca
      421 tttgactatg atttaactgg agaagtagtt cacttggaga gaaaaaatgg agacattatt
      481 aaagtttcaa ttgtacaata gaattatttt atatgaattt tatttttgaa tagttataaa
      541 taattagaaa tttgtaaaaa agtataatat attattagta atggtacaga atacacattt
      601 ataatttata gatgtgtatt ttttgtttaa aaaatttaaa tatata
//
LOCUS       DAEMIK010000343          639 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEMIK010000343 DAEMIK010000000
VERSION     DAEMIK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.349"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>639
                     /locus_tag="KPK87_003910"
     rRNA            <1..>639
                     /locus_tag="KPK87_003910"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          179 a          138 c          186 g          136 t
ORIGIN      
        1 ggatgtgatg gggagcgaaa tttagtagcg aagtagctga tttcacactg tcaagaaaag
       61 tctctatcga ggttaaaggt acccgtaccg caaaccgaca caggtgggtg aggagagtat
      121 cctaaggcca gcgagagaac tgttgttaag gaactcggca aaatgacccc gtaacttagg
      181 gataaggggt gccaccatca ggtggccgca gagaataggc ccaagcgact gtttaccaaa
      241 aacataggtt tctgctaagt cgcaagacga tgtataggag ctgacgcctg cccggtgctg
      301 gaaggttaag gggatctgtt agagcaatcg aagcagtgaa cttaagcccc agtaaacggc
      361 ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc
      421 acgaaaggcg taacgatttg ggcactgtct caacaacaga ctcggtgaaa ttgtaattcc
      481 ggtgaagatg ccggatacct gcgacaggac ggaaagaccc catggagctt tactgtagct
      541 tgacattggg tcttggtact acatgtacag gataggtggg aggctttgaa accaggacgc
      601 cagttttggc ggagccatcc ttgggatacc acccttgta
//
LOCUS       DAEMIK010000344          535 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEMIK010000344 DAEMIK010000000
VERSION     DAEMIK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.352"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(294..>535)
                     /locus_tag="KPK87_003911"
     CDS             complement(294..>535)
                     /locus_tag="KPK87_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBG2539958.1"
                     /translation="KMIKLTPTENRLLNYFIENAEKTLTIKEIYEHVWMNEYLDDNYS
                     VVVAVSGLRKKIEKDYKNPQKIITIREVGYYFNKI"
BASE COUNT          183 a           79 c           34 g          239 t
ORIGIN      
        1 gtaaaatata agtatatttt catccacaac aataaaaaaa taatagctat ataaaaatag
       61 ctactatctt tttattaatt aatattcatt tgttttatta ttacgactta tattatagtt
      121 ttatatattt tactgatgtc tataatattt cagacatttt ttatatttat tctactgcta
      181 taatttaatt aagtaaatta aatctaattt tacaaatata tcgttagaac aaaaactctt
      241 ataatatact aacatattat ctatgcctta tcattcattc tgtttataaa gctttatatc
      301 ttgttaaagt aataccctac ttctcttatc gttataattt tttgaggatt tttataatct
      361 ttctctattt tctttcttaa tccactcaca gcaaccacta cactataatt atcatctaaa
      421 tactcattca tccatacatg ttcatatatc tccttaatag ttagagtctt ttcagcattc
      481 tctataaagt aatttaataa tctattttct gttggagtta actttatcat tttat
//
LOCUS       DAEMIK010000345          534 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEMIK010000345 DAEMIK010000000
VERSION     DAEMIK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.353"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..468)
                     /locus_tag="KPK87_003912"
     CDS             complement(<1..468)
                     /locus_tag="KPK87_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBG2539959.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLIS"
BASE COUNT          221 a           54 c           46 g          213 t
ORIGIN      
        1 tgaaattaaa actgtattta ctaatggaat tattaatatc acaaatataa ataatagtat
       61 atttttagga tataacaatt ttatagagtt ctcaaatgat attttaaaga gttcaaataa
      121 ccctattttt tcatatttta tcgatttatt aaaacaaata attacagagg taaattcaaa
      181 aaatgcataa aaagctagta ttataactga tagtattatc agaactattg acatgggatt
      241 ggtcaaaact tttataaaat tatccatatt taggtagact atatgtaaac tttttattaa
      301 aatatcaaat atatatctaa tagatggcca tataaaaaat aaggctaatc ctttatgaaa
      361 taattcaaat aaaattaatg tactaaaatt atattttatt ataaaaacaa gtttacttaa
      421 aatattttta cccgtaaata taaaatttct taaaattctc ttttgcattt ttatctcctt
      481 gtatctattt gttctaattt tatcatatgt tttaaataca tttagaacat taat
//
LOCUS       DAEMIK010000346          481 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEMIK010000346 DAEMIK010000000
VERSION     DAEMIK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.354"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            108..>481
                     /gene="tcdR"
                     /locus_tag="KPK87_003913"
     CDS             108..>481
                     /gene="tcdR"
                     /locus_tag="KPK87_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG2539960.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFF"
BASE COUNT          194 a           35 c           66 g          186 t
ORIGIN      
        1 aaatttaatt tatttgccga ttatataatt ataatgactg atttaattcc aatgttgtca
       61 aaattttcaa ataaatcatc attataaata taagagagga tgattttatg caaaagtctt
      121 tttatgaatt aattgtttta gcaagaaata actcagtaga tgatttgcaa gaaattttat
      181 ttatgtttaa gccattagta aaaaaactta gtagagtttt acattatgaa gagggagaaa
      241 cagatttaat aatatttttt attgaattaa taaaaaatat taaattaagt agcttttcag
      301 aaaaaagcga tgctattata gtcaaatata ttcataaatc attactgaat aagacttttg
      361 agttgtctag aagatattct aaaatgaagt ttaattttgt agaatttgat gaaaatatct
      421 taaatatgaa aaataattat caaagtaagt ctgtttttga ggaagatatt tgttttttcg
      481 a
//
LOCUS       DAEMIK010000347          451 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEMIK010000347 DAEMIK010000000
VERSION     DAEMIK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.355"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..413
                     /locus_tag="KPK87_003914"
     CDS             78..413
                     /locus_tag="KPK87_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2539961.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
BASE COUNT          195 a           36 c           75 g          145 t
ORIGIN      
        1 gtatatttat aatattaata aatatattgc tataaaagtt tgtttaataa attaaataaa
       61 gaaaagagga gaataaaatg tacaacatag atgatatgat atataattgt ccagtagaag
      121 ctttatcaaa tattttaggt aaaaaatggg tggctataat tatatggaaa atacaagaag
      181 ataaaaaacg atttggagag ctacaaagag ctataccaga ctgtagtaag aaaatgttag
      241 ttcaacaact agatttttta attgaacagg ggattatagt caaccaaaaa aagttggtta
      301 ataatgttgt agagtcaaca tattttttaa gtgaatcagg attacgattg cttcctgtca
      361 tggaaaaaat gattatgtgg agcaataaaa atttaaattg tgataaatac taaaatttac
      421 tctgtaagta atgaaaattt aattaagcta t
//
LOCUS       DAEMIK010000348          440 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEMIK010000348 DAEMIK010000000
VERSION     DAEMIK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.denovo.356"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            149..>440
                     /locus_tag="KPK87_003915"
     CDS             149..>440
                     /locus_tag="KPK87_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2539962.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGD"
BASE COUNT          186 a           25 c           70 g          159 t
ORIGIN      
        1 ttagataata aatatatata aataataaag aaaatggatt tatagtattt aaaatttctt
       61 ataagggtaa aaaatgctat tagaaaatgt actaaaaata tttttccaat tctttataga
      121 aaaataatga tatttaggag agattgtaat gcaaattgat gtattgataa ttgatggaga
      181 taagaatagt tctcaagagc tgaaaaattt tttagaagga aaggggataa atgtctattt
      241 agcatataat tgtaaagatg ctattataaa aatatttagc aagaaatttg acttaatatt
      301 tttagagata attttagttg atggtgatgg ttgggatttt tgtaaaaaaa ttagaaatat
      361 aactacttgc ccaattattc atataactta tataaatgaa aatcaaagta ttttaaatgc
      421 tttaaattct ggaggagatg
//
LOCUS       DAEMIK010000349        14298 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.guided.114, whole genome shotgun sequence.
ACCESSION   DAEMIK010000349 DAEMIK010000000
VERSION     DAEMIK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.guided.114"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            14..238
                     /locus_tag="KPK87_003916"
     CDS             14..238
                     /locus_tag="KPK87_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539963.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            355..852
                     /locus_tag="KPK87_003917"
     CDS             355..852
                     /locus_tag="KPK87_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2539964.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            827..1333
                     /locus_tag="KPK87_003918"
     CDS             827..1333
                     /locus_tag="KPK87_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2539965.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1317..3764
                     /locus_tag="KPK87_003919"
     CDS             1317..3764
                     /locus_tag="KPK87_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2539966.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3767..5944
                     /locus_tag="KPK87_003920"
     CDS             3767..5944
                     /locus_tag="KPK87_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2539967.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            5941..6942
                     /locus_tag="KPK87_003921"
     CDS             5941..6942
                     /locus_tag="KPK87_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2539968.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            6939..7871
                     /locus_tag="KPK87_003922"
     CDS             6939..7871
                     /locus_tag="KPK87_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2539969.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            8123..10042
                     /gene="tet(M)"
                     /locus_tag="KPK87_003923"
     CDS             8123..10042
                     /gene="tet(M)"
                     /locus_tag="KPK87_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBG2539970.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10161..10328
                     /locus_tag="KPK87_003924"
     CDS             10161..10328
                     /locus_tag="KPK87_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG2539971.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(10388..10741)
                     /locus_tag="KPK87_003925"
     CDS             complement(10388..10741)
                     /locus_tag="KPK87_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539972.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            11246..11668
                     /locus_tag="KPK87_003926"
     CDS             11246..11668
                     /locus_tag="KPK87_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2539973.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            11665..11895
                     /locus_tag="KPK87_003927"
     CDS             11665..11895
                     /locus_tag="KPK87_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539974.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            12356..12559
                     /locus_tag="KPK87_003928"
     CDS             12356..12559
                     /locus_tag="KPK87_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2539975.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            12641..13858
                     /locus_tag="KPK87_003929"
     CDS             12641..13858
                     /locus_tag="KPK87_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2539976.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         4693 a         2538 c         2917 g         4150 t
ORIGIN      
        1 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
       61 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
      121 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
      181 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
      241 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
      301 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
      361 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
      421 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
      481 tatgaggaat acgccattca tgactacgag ttacccttta cggttgacga atacacttcc
      541 attggcgaac tcaatcgact atgggaaatg gtatcggaat tacccgaaga attacaatcg
      601 gagctatctg ctctgctcac tcatttttca agcattgaag aactaagcga acatcaagag
      661 gatattatca ttcattccga ttgtgatgat atgtatgacg tggcacgcta ctacattgaa
      721 gaaacgggtg ctttaggcga agtaccagct agtcttcaaa actatattga ttatcaagcc
      781 tatggtcggg atttagacct ttcaggaacg tttatctcaa ccaatcatgg gatttttgaa
      841 atcgtctatt aaatctgtcg gtacattact actggcagat tttctatttt acggggtggc
      901 tcaatcagct acccctattt tttatgaaag gattgattac atgaagaaaa tacgaagcta
      961 taccagtatc tggtctgtgg aaaaggtact gtattctatc aatgatttta gacttccgtt
     1021 tcccataacc tttacgcaaa tgacatggtt tgtcgtgtca ctctttgcag tgatgatact
     1081 tggcaacttg ccccctcttt ccatgataga gggagcattt ctcaaatact ttgggattcc
     1141 tgtggctttc acatggttta tgtctacaaa aacttttgat ggtaaaaagc cttatggatt
     1201 tttgaagtct gtcattgctt atgcactgcg accaaagctg acctatgcag gaaaaaaagt
     1261 aacgcttggc agaaaccagc cacaagaagc cattacagca gttaggagtg aattttatgg
     1321 catatccaat taaatacatt gaaaacaatc tcgtctggaa taaagacggg gaatgttatg
     1381 cttactatga gcttgttcct tacaattact catttctaag tccagaacag aaaatacaag
     1441 tgcatgattc tttcagacag cttatcgcac aaaatcgtga tggcaaaatt catgctttac
     1501 aaatcagtac agaatccagc atacgttctg cacaagagcg ttccaaaaat gaagtcactg
     1561 gcaagctcaa agcggttgcc tatgacaaaa tcgaccaaca gacagacgct ttaatatcca
     1621 tgattggcga aaatcaagtg aactaccgtt tctttatcgg ctttaagttg cttctcaacg
     1681 atcaggagtt ttctatgaaa agtcttaccg ttgaagcaaa aaatgctttg tctgattttg
     1741 tctatgatgt gaaccataag ctgatgggcg attttgttag tatgagtaat gatgaaatcc
     1801 tgcgttttca gaagatggaa aagctcttag aaaataaaat ctctcgtcgt ttcaaaatcc
     1861 gcaggttaga taaggacgac ttcggctatc tgattgaaca cctttacgga cagacaggca
     1921 ctgcctatga agagtatgag taccatctat caaagaaaaa gctggataat gaaacgctga
     1981 ttaaatacta tgacttgatt aagcctactc gctgtttggt ggaagaaaaa cagcgatatt
     2041 tgaaaatcca gcaggaagat gaaaccgtct atgtagctta ctttaccatt aacagcattg
     2101 tcggagaact ggacttcccg tcctctgaaa tcttctacta ccagcaacag caatttacat
     2161 tcccgattga tacgtcaatg aatgtggaaa ttgtagcgaa tcgtaaagcc ctatctactg
     2221 tccgcaataa aaagaaagaa ctgaaagact tggataacca cgcttggcaa agtgataatg
     2281 aaaccagctc caatgtggcg gaagctctgg aaagtgtgaa tgagctggaa accaatttag
     2341 accaaagcaa ggaatctatg tacaagctgt cttatgtggt aagggtatca gcaaatgatc
     2401 ttgacgaact caaacgtcgt tgtaatgaag tgaaagattt ttatgacgat ttaagcgtaa
     2461 aactggtacg accatttggg gatatgctcg gcttacatga agaattttta cctgccagca
     2521 agcgttatat gaatgattat attcaatacg tgacctctga tttcctcgct ggtttaggtt
     2581 ttggtgctac tcaaatgctg ggggaaaatg aggggattta tgttggctac agcttagata
     2641 ctggacgcaa tgtctatctg aaacctgctc ttgccagtca aggggttaag ggttcagtaa
     2701 ccaatgcgtt agcgtcggct tttgttggtt cgctgggtgg tggtaaatcc tttgcgaata
     2761 accttatcgt ctattatgcg gtgctttatg gggcacaagc agtgattgta gacccaaaag
     2821 cagaacgtgg cagatggaaa gaaaccttgc cagagatttc ccatgaaatc aatatcgtca
     2881 ctctgacttc tgatgagaaa aacaaaggct tacttgaccc ttatgtgatt atgaaaaatc
     2941 ccaaagattc tgaatcactg gctattgata ttctgacatt ccttacgggg atttcctctc
     3001 gtgatgggga acgcttccca atccttagaa aagccattcg tgcagtaacc aatagtgaag
     3061 tacgagggtt gatgaaagtg attgaggaat tacgggttga gaatacgcca ctaagtacca
     3121 gtatagccga ccatatcgaa agttttacag actatgactt tgcacattta ttattcagta
     3181 atggttatgt ggagcagtct atcagcttag aaaaacaact gaacattata caggttgcgg
     3241 acttggtact tcccgacaag gaaacttcct ttgaggaata taccactatg gagcttttat
     3301 ccgttgctat gctgattgtc attagtacct ttgctttaga ctttatccat acagaccgaa
     3361 gcattttcaa gattgtagat ttagacgaag catggagctt tttacaggta gcacaaggaa
     3421 aaacactatc tatgaagctg gttcgggctg gtcgtgctat gaacgctggg gtatatttcg
     3481 tgacccaaaa tacagacgac ctcttagatg aaaaactgaa aaataacctc ggcttaaaat
     3541 ttgcatttcg ttccactgac cttaacgaga ttaaaaagac cttagccttt tttggtgtag
     3601 acccagagga cgaaaacaat cagaagcgat tgcgtgattt ggaaaacggg caatgcctta
     3661 tcagtgattt atatggtcgt gtcggtgtga tacagttcca ccctgtattt gaagaactgc
     3721 tccatgcctt tgataccaga ccacctgtgc gaaaagaggt gtaaatgtga aaccatcaat
     3781 agtaaacaga ataaaatcaa actggacgct gaaacgtcta ggtaaagtgg caatgacagt
     3841 ggctttcaca cttgtgattg ccatttttct tttagccatg ctgggaacgg tggttcaagc
     3901 tgcgggcttg gtagatgata cggtcaatgt ggcaaatgaa tacagccgat acccacttga
     3961 aaactatcaa ctggattttt atgtggataa tagctggggc tggcttccgt ggaactggtc
     4021 ggacgggatt ggaaaacagg tcatgtatgg actatatgcc attaccaatt ttatttggac
     4081 aatcagtttg tatgtttcca atgcgacagg ttacttagta caggaagcct attccttaga
     4141 cttcatttcc gctacagcag attccattgg taagaatatg cagaccttag ctggtgtgag
     4201 tgcaaacgga ttttcaacag agggtttcta tgttggattc ctcttactct tgattttggt
     4261 tcttggggtt tatgttgcct atacgggact gataaagaga gaaaccacaa aggcaattca
     4321 tgccattatg aattttgtgc tggtgtttat cctatcggct tcctttattg cctacgctcc
     4381 cgactacatt aaaaaaatca atgacttttc atcagacatc agtaatgcca gtttatcact
     4441 tggcacgaag attgtcatgc cccattccga tagtcaaggc aaggacagcg tggacttaat
     4501 cagagatagc ctgttttcca tacaggttca gcaaccgtgg ctactgcttc aatacaacag
     4561 ttcagacatt gaaagtatcg gtattgaccg tgtggaaagc ctgctctcca ccagcccaga
     4621 ttccaacaat ggcgaagaca gagaaaaaat tgttgcggaa gaaattgaag acagaagcaa
     4681 taccaatcta accattacaa agaccattaa ccgtttaggt acagtcttct tcctatttgt
     4741 cttcaatatt gggatttcca tatttgtatt cctattaaca ggaatcatga ttttctcgca
     4801 ggtacttttt atcatctatg ctatgtttct gcctgtgagc tttattttaa gcatgattcc
     4861 atcatttgat ggtatgtcaa aacgagccat aacaaagctc tttaatacca ttttgacacg
     4921 agctggaatc acattgatta ttacgacagc atttagtatt tcaaccatgc tctatacctt
     4981 atcggctggt tatccgttct ttttgattgc ttttctacag attgtgacct ttgcaggaat
     5041 ctacttcaag ctgggcgatt taatgagtat gttttctcta cagagtaacg attctcaaag
     5101 tgtgggaagt cgtgtgatga gaaaacctcg tatgcttatg cacgctcaca tgcaccgtct
     5161 acagcggaaa cttggacgtt ccatgactac tctaggggct gggtctgcca ttgttacagg
     5221 taaaaaagga cagtcgggtt cggggagttc tgcaaggaca caagcagatc actcccgacc
     5281 agacggaaag gaaaaatcaa cacttggaaa acgtatcggt caaaccatcg gtacagtagc
     5341 tgataccaaa gacagaatgg tagacactgc tagtggtttg aaagaacagg ttaaagattt
     5401 gccgaccaat gcaagatatg cagtatatca aggaaaatcc aaagtaaaag agaatgtccg
     5461 tgatttaacc agtagtattt ctcaaaccaa agcggacaga gccagtggac gcaaggaaca
     5521 gcaggaacaa aggcgaaaaa ccattgcgaa gcgtcgctct gaaatggaac aggtcaaaca
     5581 gaaaaaacag cctgcttctt ctgttcatga aagaccgact acaagacaag aacaatatca
     5641 tgatgaacag acctcaaaac agtctaatat tcagacttca tataaggaat ctcaacaagc
     5701 caaacaagag cgtccagcag ttaagtccga tttttcaagt ccaaaagtgg aacgccaagg
     5761 caataccgtt caagaaaaaa ccgttcaaaa gccagcaact tcaaccacta cagcagatag
     5821 aacttcacaa cgtccaatca caaaagaacg tccgtctact gttcaaagag taccactaca
     5881 aaatacaaga agtagaccac caatcaaaac cgccaccatt aagaaagtcg gtaagaaacc
     5941 atgaagttga aaactttagt gattggtggt tctggattat tcttgatggt cttctcactg
     6001 cttctgtttg ttgccatttt attttcagat gaacaggaca gcggaatttc caatattcat
     6061 tatggaggtg tgaatgtttc cgcagaagtg ctggctcata agcctatggt agaaaaatat
     6121 gccaaagaat atggcgttga agaatatgtc aacatacttc ttgcgattat acaggtggaa
     6181 tcgggcggta ctgcggaaga tgttatgcag tcctcggaat ccctcggtct tccacctaat
     6241 tcattgagta cagaagaatc cattaagcaa ggtgtgaagt atttcagtga attattagcc
     6301 agtagcgaaa ggctcagtgt agatttagaa tcggttatcc agtcctacaa ttatggtggt
     6361 ggtttcttag ggtatgtggc taatcgtgga aataaatata cctttgaact ggctcaaagt
     6421 ttctcaaaag agtattcagg tggcgaaaaa gtgtcttacc ccaatcccat agccatacct
     6481 atcaatgggg gctggcgata caactatggc aatatgtttt atgtgcaact ggtaacgcag
     6541 tatcttgtca caacagagtt tgatgatgat acggtacaag ccatcatgga cgaagcactg
     6601 aaatatgagg gctggcgata cgtttacggt ggagcttccc cgactacttc ttttgattgt
     6661 agcggactga cacaatggac gtatggaaaa gctggaatta acttaccacg aaccgcacaa
     6721 cagcaatatg atgtgaccca gcatatccca ctatcggaag cacaagctgg cgatttggtt
     6781 ttctttcatt ctacctataa cgctggctct tatattactc atgttgggat ataccttggc
     6841 aataaccgta tgtttcatgc aggcgaccca atcggttatg ccgacttaac aagcccctac
     6901 tggcaacagc atttagtggg agcaggacga atcaaacaat gagaaaggaa gatttaatga
     6961 tgaaatttag aaaaaatcag aataaagaaa aacagatacc aaaggaaaag aaacctcgtg
     7021 tctataaggt caatcctcat aaaaaggttg tgattgcctt gtgggtactt ttagggctta
     7081 gtttcagctt tgcgatattc aagcacttta cagctataga tactcatact attcacgaaa
     7141 caactatcat agaaaaggaa tacgttgata ctcatcatgt agaaaatttt gtagagaact
     7201 ttgcgaaagt ctactattca tgggagcaat ccgataagtc cattgataat cgaatggaaa
     7261 gtctaaaagg ctatctgaca gatgaacttc aagctctcaa tgttgataca gtacgcaaag
     7321 atattcctgt atcgtcttct gtaagaggat ttcagatatg gacggtagag ccaactggcg
     7381 acaatgagtt taatgtaacc tacagtgtag accagctcat tacagaggga gaaaatacaa
     7441 agaccgtcca ctctgcttat atagtgagtg tctatgtaga tggttctgga aatatggtac
     7501 tggttaagaa tccgaccatt accaacatac ctaagaaatc aagttataaa ccaaaagcca
     7561 ttgaaagtga ggggacggtt gattccatta caaccaatga aatcaatgag tttttaacga
     7621 cgttcttcaa gctctatcct acagcgacag ccagtgaact ttcctactat gtgaatgacg
     7681 ggatattaaa accaatcgga aaagagtaca tctttcaaga actggtaaat cctattcaca
     7741 atcgtaagga taatcaagtc acggtatcgc tgacagtgga gtatatcgac cagcagacca
     7801 aagcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
     7861 ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg tactcttttt
     7921 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtgacta
     7981 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
     8041 cgggaacaaa taattagatg tcctttttta ggagggctta gttttttatg cccttttggg
     8101 tttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga
     8161 tgcaggaaaa actaccttaa cagaaagctt attatataac agtggagcga ttacagaatt
     8221 aggaagcgtg gacaaaggta caacgaggac ggataatacg cttttagaac gtcagagagg
     8281 aattacaatt cagacaggaa taacctcttt tcagtgggaa aatacgaagg tgaacatcat
     8341 agacacgcca ggacatatgg atttcttagc agaagtatat cgttcattat cagttttaga
     8401 tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt
     8461 tcatgcactt aggaaaatgg ggattcccac aatctttttt atcaataaga ttgaccaaaa
     8521 tggaattgat ttatcaacgg tttatcagga tattaaagag aaactttctg ccgaaattgt
     8581 aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga
     8641 acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata tgtccggtaa
     8701 atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga attgttctct
     8761 gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc ttatagaagt
     8821 tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt gcggaaatgt
     8881 tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tatatacgcc tttatagtgg
     8941 agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga
     9001 aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat
     9061 tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt
     9121 gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag
     9181 taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc
     9241 gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa
     9301 agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact
     9361 aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca
     9421 catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc
     9481 gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt
     9541 tcaaaatgca gttatggaag ggatacgcta tggctgtgaa caaggattgt atggttggaa
     9601 tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg ttagtacccc
     9661 agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa aagctggaac
     9721 agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat atctttcacg
     9781 agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat tgaaaaataa
     9841 tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc gtagtgattt
     9901 aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt accatgttac
     9961 taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata aagtacgata
    10021 tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg tttcttgtta
    10081 aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag gagatagtta
    10141 ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga ttaaagataa
    10201 gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga caagaaaatt
    10261 taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca aagacgcaga
    10321 gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta tgtatggatt
    10381 cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa gattccatta
    10441 aggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat cgtcgggtgc
    10501 tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga agtaacgata
    10561 caaggtcata aagaacttgt atgctggggt gttgcccttt attttcaata ttagttaagt
    10621 accgtgggtc aatttcaatc aatgctccca cttgttcacg agttaaacct cgtttcaatc
    10681 gagcttcttt aatggctaaa ccaaaggctc taaaatcata tttatcttct tttttacgca
    10741 tagtagacca cctctataca ttttattgtt cctactgaat taaaaacagg tatagaaaaa
    10801 cgtgttatat ggtttatagg tttatattta ataaaaagca ctactaaacg ccaataaaaa
    10861 aaaccgttat atggtagtgc tatttacgct gttaaaatat tgtatattac ttccaaatgg
    10921 cggtttgttg gaggtcaacg tcgccatgaa gtacatcata tacaataaat ttccttacat
    10981 tgggttcttg tcaaaaaaag tcgtctatct gcaatagata agtacgtcca ccaatgtggt
    11041 tttataaatc atatagatag aataacagaa gcatgtaaac agagaaataa atctgtttat
    11101 atgctttttt ggctattcag aactttttta caaagtttat ttatcagtaa tgcaacaaat
    11161 ccccctttca cattgggact aagagtgaaa ggagataaac gagcaaggct cacttccttt
    11221 cctagacaga aagggggtga gaaacatgaa accatcttct tttcagacca caatagaaaa
    11281 tcagtttgac tatatctgta aacgtgctat ggaagacgag cgaaagaatt atatgcttta
    11341 tctttcaagg attgcaaagc gtgaggtgtc cttttcggat gttggcgatt atcttgttag
    11401 ccagtttgcg acaacagata actattcaac tgactttcag atttttacac tcaatgggtt
    11461 atcagtaggc gttgaaaatg atttgttgag tgaagcatta cgtgagttgc cagacaagaa
    11521 acgtgaaatt ctactgctgt tttactttat ggacatgagc gattcagaaa ttgcagacct
    11581 gttgaaattg aaccgttcta ctgtctatcg gcatagaacc agtggactag ccttaattaa
    11641 aaagtttatg gaggaatttg aagaatgaaa acacaatatc ctatgattcc ctttcctctc
    11701 attgtaaagg caacagatgg cgataccgaa gcgattaacc agattctaca tcattacaga
    11761 gggtacataa cgaagcgttc cctacgactt atgaaagatg aatatggcaa tcaaagtatg
    11821 gtcgttgatg aagtcttacg tggaagaatg gaaaccagac tgattacaaa gattttgtca
    11881 tttgaaatta agtaatatcc tctctccttt cgtggaagcg tgctaaacca ttccacgctt
    11941 cccgaacagg gaggtttgtt attccaccaa agcatattga gctttcaatg tgttttgata
    12001 ggctaacgag ccattgttct ttgaaaactg aataaaagta atcgaatacg tttcgataag
    12061 aaaagagcca acggaactaa ccgccatgac ctatcttata aagatagcga gcgattcatg
    12121 ttagtgatcc gagaagcaat ctttagcagg attgcctgca acgacattct tatcgtgata
    12181 atgatactcc catacagtca atagtccgag cgtgataaaa ccgtcgcagg caatgagtat
    12241 ggctacatga gaaccatgca ggggtggaac tcccgtgagc tttgctaaag ctgttcgatt
    12301 gctggtaaaa caacttttat gaaatccaaa taagtgattt ggaaaggagg attttatgaa
    12361 gcagactgac attcctattt gggaacgtta taccctaacc attgaagaag cgtcaaaata
    12421 ttttcgtatt ggcgaaaaca agctacgacg cttggcagag gaaaataaaa atgcaaattg
    12481 gctgattatg aatggcaatc gtattcagat taaacgaaaa caatttgaaa aaattataga
    12541 tacattggac gcaatctagc gtcgccaaag ggtcttgtat atgataaaat agtattaagt
    12601 cgtatcaagg ctctttccat aaaggaaagg agcaaatgcc atgtcagaaa aaagacgtga
    12661 caataaaggt cgaatcttaa agactggaga gagccaacga aaagacggaa gatacttata
    12721 caaatatata gattcatttg gagaaccgca atttgtttac tcgtggaaac ttgtggctac
    12781 agaccgagta ccagcaggaa agcgtgattg tatctcactt agagagaaaa tcgcagagtt
    12841 acagaaagac attcatgatg gtattgatgt tgtaggaaag aaaatgacac tctgccagct
    12901 ttacgcaaaa cagaacgctc aaagaccaaa ggttagaaaa aacactgaaa ctggacgcaa
    12961 atatcttatg gatattttga agaaagacaa gttaggtgta agaagtattg acagtattaa
    13021 gccatcagac gctaaagaat gggctattag aatgagtgaa aatggttatg cttatcaaac
    13081 catcaataac tacaaacgtt ctttaaaggc ttcattctat attgctatac aagatgattg
    13141 tgttcggaag aatccatttg actttcaact gaaagcagtt cttgatgatg atactgtccc
    13201 taagaccgta ctaacagaag aacaggaaga aaaactgtta gcctttgcaa aagctgataa
    13261 aacctacagc aaaaattatg atgaaattct gatactctta aaaacaggtc ttcgtatttc
    13321 agagtttggt ggtttgacac ttccagattt agattttgag aatcgtcttg tcaatataga
    13381 ccatcagcta ttgagagata ctgaaattgg gtactacatt gaaacaccaa agaccaaaag
    13441 tggcgaacgt caagttccta tggttgaaga agcctatcaa gcatttaagc gagtgttagc
    13501 gaatcgaaag aatgataagc gtgttgagat tgatggatat agtgatttcc tctttcttaa
    13561 tagaaagaac tatccaaaag tggcaagtga ttacaacggc atgatgaaag gtcttgttaa
    13621 gaaatacaat aagtataacg aggataaatt gccacacatc actccacata gtttgcgaca
    13681 tacattctgt accaactatg caaatgcagg aatgaatcca aaggcattac agtacattat
    13741 gggacatgct aatatagcca tgacgctgaa ctattacgca catgcaacat tcgattctgc
    13801 aatggcagaa atgaaacgct tgaataaaga gaagcaacag gagcgtcttg ttgcttagta
    13861 gtacaaatga atttactact tatttaccac ttctgacagc taagacatga ggaaatatgc
    13921 aaagaaacgt gaagtatctt cctacagtaa aaatactcga aagcacatag aataaggctt
    13981 tacgagcatt taagaaaata taaaaagata attagaaatt tatactttgt ttaatataaa
    14041 aattttatta aatttagtat gtaatttttt aatcttattt tattccatct atagtcttat
    14101 gctaaataat aactttgata atatttccct aacttatgtt tcaactggtt tatattgcaa
    14161 tttatttaat tacagcttat ttgcataata attcgtccat atcataagtt gtgtatttat
    14221 ataaatacac aattaaaaat aaatcactat ggtatactaa ttattacgaa gactatactt
    14281 atatgcaaag ataaatat
//
LOCUS       DAEMIK010000350        12049 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.guided.133, whole genome shotgun sequence.
ACCESSION   DAEMIK010000350 DAEMIK010000000
VERSION     DAEMIK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.guided.133"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            210..2741
                     /locus_tag="KPK87_003930"
     CDS             210..2741
                     /locus_tag="KPK87_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2539977.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3175..3876
                     /locus_tag="KPK87_003931"
     CDS             3175..3876
                     /locus_tag="KPK87_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG2539978.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            3926..5008
                     /locus_tag="KPK87_003932"
     CDS             3926..5008
                     /locus_tag="KPK87_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2539979.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            5269..6369
                     /locus_tag="KPK87_003933"
     CDS             5269..6369
                     /locus_tag="KPK87_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG-Cd"
                     /protein_id="HBG2539980.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLISDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            6366..7172
                     /locus_tag="KPK87_003934"
     CDS             6366..7172
                     /locus_tag="KPK87_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2539981.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7190..9328
                     /gene="vanT"
                     /locus_tag="KPK87_003935"
     CDS             7190..9328
                     /gene="vanT"
                     /locus_tag="KPK87_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG2539982.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            9642..10262
                     /locus_tag="KPK87_003936"
     CDS             9642..10262
                     /locus_tag="KPK87_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG2539983.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(10428..10964)
                     /locus_tag="KPK87_003937"
     CDS             complement(10428..10964)
                     /locus_tag="KPK87_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2539984.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11077..11751)
                     /locus_tag="KPK87_003938"
     CDS             complement(11077..11751)
                     /locus_tag="KPK87_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG2539985.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
BASE COUNT         4541 a         1315 c         2058 g         4135 t
ORIGIN      
        1 gttacttttt ataaaataag aatagataaa gataaaaatg aatatgacat aaaagtagat
       61 tctaaaaagg gaactgtatt gaaggtagaa aaagaagatt agaagattta aactatcttt
      121 aaaataattt cttttttgaa taaattttgt ttaaaatata acaagcaggg tataaagaga
      181 ctaaataact attttaggag gagtacggta tgtcattgaa atcactaaaa ataaaagaaa
      241 atttatattg ggtaggttca cttgaccctg atttaagagt atttgacata attatgtata
      301 caccttatgg gactacttat aattcatatg tattaaaagg aacagaaaaa acagttttat
      361 ttgagacagt taaagataag cattttgaca attatattga aagattaaat gatttaaata
      421 tagattttga aaaaatagac tatattgttg taagtcatac tgaaccagac catgcaggaa
      481 gcgttgaaaa gcttttggat ttagctaaaa atgcaaaagt agtagcttca gaaacagcaa
      541 ttaaatatct taaggaaatc gtaaacaaag actttgaata tgttgcagtt acagatggag
      601 atacattatc aattggggac aaaactttag aatttttctc tgtacctatg cttcattggc
      661 cagataccat atacacatat ataaaagagg ataaaactct tgtaacatgt gattcatttg
      721 gtagtcacta tagtaatgat aagatagtaa atacacttga tgaaaatgaa gaaaaggatt
      781 acttggatgc tttaagatac tattatgatt gtataatggg tccatttaaa ccatctatgg
      841 taactgctat agaaaagata aaagatttag atatagatac tgtatgtcca ggtcatggtc
      901 cagtattaac tgaaaatcct agaaaaatta tagaccttta ttacaactgg agtgtaaatg
      961 aacaaataaa gttagaaaaa gaagttacaa tttgttatgt ttctgcacat ggatatacaa
     1021 agattatggc agaagctata aaagcatata ttgaaaaaaa tagcaactat aaagtaaatt
     1081 tatttgatgt aatagagcat aagcaagaag atattttggc aaaaatagca gtatcacaag
     1141 gtgtattatt tggaacacca acaatattag gagatgcgtt aaagcctata tgggatatat
     1201 taatatcgtt aaacccagtt cttcatggtg gaaaagttgc ttcagtattt ggttcatatg
     1261 gatggagtgg agaaggtata gaaaatgcta tggagagaat aagtcagctt agaatgacag
     1321 cagtaaaacc ttttgctgtt aactttaaac catcaaatga agagattgat aaattacatt
     1381 cttatacagg taaattccta gataagttaa attctacttt tggaagtaaa aagaaaacta
     1441 agaaatttaa atgtgtaatt tgtaatgaag tttttgaagg tgatagtgct cctagtgtat
     1501 gccctgtatg tggagctaag gaagaccaat ttatagaagt tgaagaagat gaagttactt
     1561 ttagaaaaga tactgatgaa tatttcgtaa tagtaggaaa tggagcagct ggtttttatg
     1621 cagcagatgc tataagaaaa agaaataaaa cttgtaagat aactatgatt tctaatgaag
     1681 atgagttgac ttactataga cctgcacttt ctgatggtat aaatgaagaa cttggttcag
     1741 atttctacat ggaagataaa gattggtatg ataaaaataa tatagtagta atattaggaa
     1801 cgaatgtaga taaattagat gaagtgaata aaactattat agtgaatgat ggagctatta
     1861 aatttgataa gcttgtaata gctacaggaa gtagaaactt tataccacca attaagggcc
     1921 atgatttaga aaatgtgttt actcttagaa atataaaaga tttatatagt gtaaaagaag
     1981 cactagaaaa atctaagaaa gttgtagtga ttggtggcgg tttattggga cttgaagctg
     2041 catgggaatt tagactaaaa ggattagaag tagtagtagt tgaagccatg gatagcatac
     2101 tatcaaaaca acttgataaa gaaggtagta aaatattaga acaatgtgta agagatacag
     2161 gaatagatgt aagattgggt gtagcagttg atggtattga aggtgatgga aaagctcaaa
     2221 aagttgtatt taaagatgga gatagtgtcg actgtgatat ggtagtattt tcaattggtg
     2281 taagagcaaa cactcaaatg gttcaggata catctgtaaa aattgataga ggtattgttg
     2341 tagataagac attacagaca aatgttaaag atatatatgc ttgtggagat gttgcacaag
     2401 tgggaaatat tagtttagca atatggccat cttcagtaga aatgggaaaa atagcagggg
     2461 caaatgcaag tggagataat ttgacatttg aaagtgaagt atatccagtt tcattagatg
     2521 caatgaatgt aaaagtattt agtattggta atatacaaaa ttttgataaa gaaatttcat
     2581 caaaagatga aggtcaaaga atatataaaa aattatttat gaaagatggt tcattagttg
     2641 gagctatact tataaatgat ttaagctgta ctgttaaatt gataagatta atatctgaaa
     2701 aaggtgattt tgaggatatt atgaaggctg atatactata atttaaatac attaaaataa
     2761 aaaggagttg tcttaaaatt gatttttttc taatcaaatt ttaaattcaa ctcctttttt
     2821 gaatttttat ataaaataac atggaaattt tagtaatgtc tgtggttaca tacataggtt
     2881 taattgtttt ttatatggat tgtctctggt ataataaatt gaattgtgtt tgactgatag
     2941 atatttttat tgaaagttaa tacctgttat gcgataataa ggacgtgtta ggggagtttt
     3001 tataataaca attaatctca gtttttgtgt tccaatagta gcatcaacac attctgatgt
     3061 agtagttctt ttcttataaa aattagttac aaatatgctt gtaattattt gatatgtaaa
     3121 tttaccaata ttataaatga tgacctcgat aataaggaga gtgaaaaata atctatgaac
     3181 gaaaatattt tgattgtgga tgatgaaaaa gaaatagcag atttaattga agtatattta
     3241 aaaaatgaag gttttacagt atacaagttt tatacaggta aggaagctct agaatgtatt
     3301 gaatctaaaa atttggatat ggctatttta gatattatgc ttcctgatat tgatggattt
     3361 catatttgcc agaaaattcg agaaaaatac tattatccta tcattatgtt aacagctaaa
     3421 attgaagata tagacaagat aatgggttta accataggtg ctgatgatta tataacaaag
     3481 ccatttaacc ctttggaagt tgttgcaaga gtaaagacac aattaagaag atatatgcga
     3541 tataataatt cttatgaaca acagtcgata attgttaatg agtatgatat taaaggattg
     3601 attatcaata aagaaacaca taaatgtagt ttatttggga aagaagtagc attaacaccg
     3661 attgaatttt ctattctttg gtatttatgt gaacatcaag gaaaagtggt tccttctgaa
     3721 gaattgtttg aagctgtatg gggagaaaaa tatcttgata ataataacac agttatggct
     3781 catatagggc gattgcgtga aaaaattcat gaaccttcta aaaacccgaa atttataaaa
     3841 actgtatggg gagtaggtta tacaattgaa aagtaaaaat attaaagtca atcctgactt
     3901 tactcaagtg agaaataaaa tttttatgag aacagcttta atggcattta tagccattat
     3961 tttcatatgg attttatatt cttttatatt gtatggtaag ttttctatct ggatagtatc
     4021 attctttgaa aatgcttttc gtatggaata taaagctgca ttgaatttat atcagcagat
     4081 tttccgtaat agcatggagc tcttcatgat actagctatg gtgattgtgt tttttataat
     4141 attccgtgtt tatttaaatt ggtttacaaa atattttagt gaaataaatc aaggtattga
     4201 ctctcttatc aaagaagatg ttggagaagt ggctttatct ccagagttat tggcaataga
     4261 aaagaaaata aactcaatta aacatatact tgaacaacgt aaatttgaga cacaaatggc
     4321 agaacaacgt aagaatgaac ttattgttta tcttgcacat gatttaaaaa caccacttac
     4381 atcagttata gggtatctga cactacttcg agatgaaagt cagatttctg aagaactgcg
     4441 aaaaaaatat ttatctattt cgttggataa ggcagagcgt cttgaagatt taattaatga
     4501 attctttgaa attacaaggt ttaatttatc aaatatatca cttgaataca gtacagtaag
     4561 cttaactcgt atgcttgaac aactaacata tgagtttaaa cctactttga tgagtaagaa
     4621 tttaaaatat acactgaatg ttacaccaaa tatgatgata aggtgtgatg ttaataaaat
     4681 gcaacgtgta tttgataact tattacgaaa tgctgtttat tatagttttg aagatactac
     4741 tattgaaata acagctgtac aggaggatga ttatataaag cttaaattta tgaatcatag
     4801 caatacgatt ccagaggaaa aacttgagcg tatttttgaa caattttaca ggttggatac
     4861 atctcgtaat tcaaacagtg gtagagctgg gttgggactt gctatagcta aagaaatagt
     4921 ggagcttcat aacgggacaa taacagctca tagcgagaac aatataatta cctttaaggt
     4981 tattattccg attgtaggaa atttgtaaga atttaagtat aaaaaaatag gaaattcttt
     5041 aggataaata aataaagagc actcttgtta ttgatataat tactgtatca aaacaagagt
     5101 gcttttattt tgatacagta attatatcaa tagaaaaatg tgaaaatata ttatgtggaa
     5161 cagatgcaat tgtaagtgaa ggttgattgg atttgaaata gaaaaagtat tttaacatgt
     5221 ttctgtagat tgtataaaaa taaataaatc aacaaaggag aatcaaagat gaataataaa
     5281 aaaattgcag ttatatttgg tggaaactca acagaatatg atgtatcatt acaatcagct
     5341 tttgcagtac ttgagaattt gaatacggaa aaatatgata ttctaccaat tggaattaca
     5401 agggagggta attggtatca ttatattgga gattataata atatccagaa taacacttgg
     5461 tttgaaaggg atactgatat aattccagtt attgtttcgc agaaccgtgt caaaaatggt
     5521 attattgaat ttttaatttc tgatgctgtt tctcagaatc atattccaaa tagtgctatt
     5581 gaatcatgtg aaaatgatat taaatacact aaacttgatt ttgtatttcc tgtattgcat
     5641 ggaaaaaatg gagaagatgg aacagtgcag gggttatttg aattggcagg tatacaggtt
     5701 attggatgtg gtactctttc ttcagcatta tgtatggaca aagacaaatc acataaactt
     5761 gttcattcag aaggtattaa agtaccaaaa tcaatattac taaaatactc agataaattt
     5821 aatgtggctg aactgtcaaa agacttaact tatccattat ttataaaacc tgtgcgtgca
     5881 ggttcatcat ttggtattag taaaatttat aaaaaagaag agttacaagt agcagtagac
     5941 ttggcttttg aacatgataa tgaagtaata attgaggaaa atattgatgg ttttgaggta
     6001 gggtgtgcag ttttaggaaa caatgactta cttgttggaa gagtagatga aatagaattg
     6061 tcaaaaggat tttttgatta tacagaaaaa tatacattga aaagttcaaa aattcatatg
     6121 ccagctagaa ttgatttgga ttgtgagaaa agaatacaag agacagcaaa aattatttat
     6181 agaactcttg gatgttctgg atttgctaga gtagatatgt ttttaactcc atctggtgaa
     6241 attattttta atgaagtcaa tacaattcct ggctttactt ctcatagtcg ttatccaaat
     6301 atgatgaaag gtattggact gtcttttgaa gaaatattgg acaagataat tgaggtgtac
     6361 ataaaatgat gaataatatt ttattagata aaaataaaga agataaaatg acaaatacta
     6421 tcttattgac taaagataaa gtttatgatg ggaatctaat tctagtaaat gcttttttac
     6481 cagttaaaac atctgaagat atagatttaa ttcctgtaga tacaaggttt ccaagtattc
     6541 ttatgaaacg tgaagctaca aacattttac aaaatatttt aaaaagtata tgtggaataa
     6601 atgaaatagt acctgtaagt ggatatcgta ctgcagagga acaacaagat atatattcaa
     6661 gttctctgcg tgacaatgga aaagacttta ctaaaaagtt tgtagcactt ccaaatcaca
     6721 gtgaacatca gacagggctt gctattgatt tagggttaaa gaaagaagtt atagatttta
     6781 tctgccctga atttccttat aatggtattt gtaatgagtt tcgtaaggca gcactacatt
     6841 atggttttat agagcgttat cagtgtaaaa aagaagaaat aacaggaata tctcaagaac
     6901 catggcattt tcgttatgtg ggatatccgc attctgagat tatcaataaa atgaatctcg
     6961 ctcttgaaga atatatagaa tatattaaga gattttcgta tacaaattgt ttaaaaatag
     7021 aagataaaga aaaaattata gaaatctttt atgtacctat tcatactaaa gaagaagtga
     7081 gaatctctat acctgaacaa tgtgtatatc aaatatcggg taacaataag gatggtttta
     7141 ttgtgacatt ttggagaaat agaaaggact aagaaatgga ggaacaagaa tgattaggaa
     7201 taaatcttat gcagggcttg attattttag gattatagct gcattactta taattgcaat
     7261 tcatacatct ccattattat catatagtaa aatggctgac tttgtgttaa ctagtataat
     7321 tgctcgtatt gctgtgccat tcttttttat gctatcaggc ttttttctaa tttcaacata
     7381 taattataat tgtaataaac tgaaatcttt tctaaaaaat gtattattta tttacgggat
     7441 agctattgta atttatattc ccttaaatat ttacactgga tatttttcca tgaataattt
     7501 agtgcccaat attattaagg acattgtatt tgatgggact ttatatcatt tatggtatct
     7561 tcctgcatca attattggtg caagtattgc atactttctt gtaaaaaaac taggtttaaa
     7621 gtactcacta gtaatcacta ttgttattta tgtaattggt atgtttggtg atagttatta
     7681 tggtttttta gaaaaaatac cactattaaa gaatttatat gggtatattt ttgaagtatc
     7741 agattataca cgaaatggta tattttttgc acctgtattt ttcatacttg gtggaataat
     7801 atctaataaa tctaattata tgtcaactaa aaatagcttg attggatttc ttatatcatt
     7861 cttacttatg ttatgtgagg gaatgttttt acataaacta ggggtacagc gtcatgatag
     7921 catgtatatt atgttattgc catgtatgta tttcttattt acagccttaa catttttgaa
     7981 aggttctcga gctaggttat tgcgaacatc atcattaatt atttatatta tacatcctat
     8041 gatgattgtt gttattcgta tgttttctaa aatattcgga atacaaactg tcttggttga
     8101 caatagcctt attcattata tagtggtaag tatagcatct tttatagctt ctataattat
     8161 tatattgatt cttaaacctc taaaaaaaga cttgaaagga gaacataagg taaataccaa
     8221 tagagcttgg attgaaattg atttaaataa tttggaacat aatgtaaaga tgatacaaaa
     8281 agctatgcca gaagattgtg aattgatggc agttgtaaaa gctaatgcct atggtcatag
     8341 tgcttttgaa gtagcgactt gtgcaaacca aataggtgta aaagcatttg cagtagcaac
     8401 aatagatgag gggattgatt tacgtcgata tggaatacta ggagaaatat tgattcttgg
     8461 atatactaac ccaataaggg caaaggaact tcataaatac aatcttacac agagtgttat
     8521 tgaccttaat catgctatct ctttaaacaa acagaaatac aacataaaat cacacattaa
     8581 aattgataca ggtatgcaca gactgggttt tgatgttaat gatgtgaggt caattttaaa
     8641 ggcatttaaa ttaaaatact tagatatatg tggcatattt acacacttgt gtgtttcaga
     8701 tagtctgaaa gatgaagatg tagactttac aaataaacaa attgaatgtt tttataagtt
     8761 aattgatttt ctgttaaaaa aaggtataaa aattcctaaa gtacatattc aaagtagtta
     8821 tggttttttt aattatccca atcttaagtg taattatgta agaattggaa ttgcattata
     8881 cgggatatta agttctccaa atgatacgac taaattgcaa cttgatttac gacctgtttt
     8941 atccttaaaa tcacaagtaa ttcttattag aaaaatacaa aaagatgata gttttggata
     9001 tggaagagtc tttattgcaa gcagagatag cgtaattgct atattatcta ttggctatgc
     9061 cgatggtata cctagaaatt tatcctgtgg aaaaagttac gtaataataa atggttgtag
     9121 agcagcaata gtgggtcgaa tttgcatgga ccaacttgct gttgatatta cagatatacc
     9181 taatgtagaa gttggtaata cggcaataat aataggaaga gataatttat ctgaattatc
     9241 tgcttctgaa gttgcaaata actcatgtag tatatcgaat gaattattga gcagagtagg
     9301 aagaaggtta aatgttataa agaagtaact caattaaata gtaatatttg ttgtactgtg
     9361 taatttattt tactctgact agacattttt agtaatatta tgtctaatat atatttttta
     9421 taacttacat gacaatttaa gttataaaaa ggtgatgttt cagattttta ttttgaaaca
     9481 tcaccttttt tgttataata tataataaaa taattatacc ttaactgtaa acttatatag
     9541 attatttact ttttaatatt agattcattt tttgagattg gtatataata aacatataga
     9601 gtatataata atagttaaag ttaaaaaagg ggaaaagcaa tatgcagtat atacaagaac
     9661 tacttgattt gactcaaaat tattgggtgt tagccacaat aatcggctta ttaagtgcat
     9721 ttatagaaag ctttatacca gccttaccac ttgttgctat agttacagca aatgctgcaa
     9781 tacaaggatt atttattgga tgtttgttat catggattgg ctcaggactt gggacaactt
     9841 cattattctt attaataagc agatttaatg atagtaagct ttttaataag ttgagaaata
     9901 gtaaaactga aaaagcaata agttggatgg ataaacaggg atttaagtta ttatttatag
     9961 cgtatgcctg tccatttatg cccggttgtt tagttactat cgcatcagct tttggtaaaa
    10021 aagatttaaa agattttgtg ccagcaatgt tggcaggaaa atttgtaatg tttatagtta
    10081 taagttacgt ggcaagtgat atagaaggtt ttataacaag tcctttaaag atagcatcat
    10141 ttatattatt ggtgtttttg tcatggaaga ttggaaatag agtaaataaa aatcttgaaa
    10201 atcataatta tgatttccat cataaaaaac atcacaatga taaagatgat aaaatgattt
    10261 agcttgtacg aaataaaacg tatcttaaaa tgatttagta ctaaattatt ttaagatacg
    10321 ttttattttt tgtattaagg tacaaaattt ttagttcttt tgtaaactaa aatatataaa
    10381 acttatttat acttagttca aaatattgtt atttattttt tctacaacta taataaaata
    10441 taaaattgat atccaatcaa aaaaataact atgaaattta atagcttaga tatgaatttt
    10501 tctgtttttg agttagtttt tataggtagt aaagaaatgt ttatggtaat gggataaaac
    10561 aattcaatac cttctttagt aataaggtca agacacaagt gagatagata tcctattata
    10621 aacccactgg acaaacatga gaaaactata ttattgttag tgaagattgt cagagattta
    10681 aaaaaactat aaataagtaa taaggcaatt agactgtgag taaaccctct atgtctaaac
    10741 ctacttccaa ataatttgta tattaacata aattttttac ttatgtaaga acctctcata
    10801 tctatatctg gaaataaaga acctaaatat gcaaaataaa tataaattat aattaatact
    10861 attttatatg caatatcgta tttaactagg tatagattgt ttataaaggg gagtatcaaa
    10921 agtgcgaata tataacctcc cctttcatgt gtttctttta tcatataaaa atcctcctga
    10981 atcgtcacta taaacattat attggtaaat ttagactatc ttttattata ttatatagca
    11041 aacataagtt tttttcaaaa ggattttaat atttaattaa tcttgagatt ttaaattttt
    11101 gtaattttct aaagcaccat tccaatttac tacattaaac cagttgtcaa tgtattcatt
    11161 tcttctattt tgatatttta aatagtaagc atgctcccaa acatcaagtc ctattatagg
    11221 agttaggttt ttacttacag ggctatcctg atttggagta gtcataatag ataatttccc
    11281 atctttagta gctacaagcc aagcccaacc agaaccaaag acatctaaag cagatttttg
    11341 gaactcttgc ttaaattttt caaaagaacc aaagtctcta tcaatagctt cttttaaaga
    11401 ttcagaaggt atggtttttt ctggcgtcat tatatcaaaa aagaatttat gattataagc
    11461 tccacctgca ttatttctta cagttgtagc aatatcttta ggtaaagaat ctaaattttg
    11521 caataattca cataaagaat aattataaag ctcaggatat ttttcaagag cagcatttaa
    11581 tttatcaaca taagcttgat aatgcttatc atgatgcagt ttcattgttt ctttatctat
    11641 ataaggttca agtgcatcat atgcataagg taatggttta accttaaatt tgttattttc
    11701 aggtgtaaaa gcaaatgaag ttatgcactg tgagattata aataaagaca taataactgg
    11761 tattaatatt tttttcttca taaaaatcac ctcctaatat agtatttcta gaaataaaaa
    11821 aaatatgaaa tattaacaga taatttataa gaatttaata gcaatttcat tttaaattga
    11881 ataagtttta aagccaaaga caaaactatg aaatatagaa taagactaaa ggacagaaag
    11941 gaattttgat tatatggacg gcaatatttc aatttattat aaaggtgata tatctaaaaa
    12001 agaagatatt aaatttaaaa taaaagaatc tgtaaataat acaagactt
//
LOCUS       DAEMIK010000351         8776 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.guided.160, whole genome shotgun sequence.
ACCESSION   DAEMIK010000351 DAEMIK010000000
VERSION     DAEMIK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.guided.160"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..457)
                     /locus_tag="KPK87_003939"
     CDS             complement(<1..457)
                     /locus_tag="KPK87_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG2539986.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKK"
     gene            complement(1127..1942)
                     /locus_tag="KPK87_003940"
                     /pseudo
     CDS             complement(1127..1942)
                     /locus_tag="KPK87_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2043..2741
                     /locus_tag="KPK87_003941"
     CDS             2043..2741
                     /locus_tag="KPK87_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2539987.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAKRGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            2829..3449
                     /locus_tag="KPK87_003942"
     CDS             2829..3449
                     /locus_tag="KPK87_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2539988.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            3605..4387
                     /locus_tag="KPK87_003943"
     CDS             3605..4387
                     /locus_tag="KPK87_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG2539989.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            4512..5438
                     /locus_tag="KPK87_003944"
     CDS             4512..5438
                     /locus_tag="KPK87_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539990.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            5452..5901
                     /locus_tag="KPK87_003945"
                     /pseudo
     CDS             5452..5901
                     /locus_tag="KPK87_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            6260..6667
                     /locus_tag="KPK87_003946"
     CDS             6260..6667
                     /locus_tag="KPK87_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2539991.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRINDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKKLKELMEEH"
     gene            6669..6899
                     /locus_tag="KPK87_003947"
     CDS             6669..6899
                     /locus_tag="KPK87_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2539992.1"
                     /translation="MKPTKEVLPFTIILLAVHSDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(7087..7224)
                     /locus_tag="KPK87_003948"
     CDS             complement(7087..7224)
                     /locus_tag="KPK87_003948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2539993.1"
                     /translation="MVFQPIPFLWAEPCHTGVSLYCGTGYGKTTVPKVIHKSLYSSSL
                     D"
     gene            7289..7492
                     /locus_tag="KPK87_003949"
     CDS             7289..7492
                     /locus_tag="KPK87_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2539994.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            7570..7923
                     /locus_tag="KPK87_003950"
     CDS             7570..7923
                     /locus_tag="KPK87_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG2539995.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            7995..8627
                     /locus_tag="KPK87_003951"
     CDS             7995..8627
                     /locus_tag="KPK87_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG2539996.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         3128 a         1302 c         1420 g         2926 t
ORIGIN      
        1 ctttttttgg agttttatta aaatcatgat tagatataat tactttcact tcttttttat
       61 gagcaaaatt aactacttca tctatgacct catctcccat aaataactct acatctatta
      121 aatctactaa acctgtatta gaaatttctt tattcaaagt agtataataa tctcttgata
      181 ttaatttttc tcccccttct actacacttc taaatgtgaa taataaaggg atatcatgta
      241 tataacttct taattcatag agaacttcct taacttcttt aatattttct acattctcaa
      301 aaaaatcaac tcgccactct ataatatcca aacatgcatc ttttaactct ttagcctctt
      361 ttataatatc ttttttattt ttcccaataa ttggtacaca aattttaggt ctaccttctc
      421 caatagtaat attttttact tgaacttttc ttttcatatt ctcctccata atcagaaatt
      481 ctataggact ttaaattcta ttttctgcac aatttaatta tacattaaaa atactataca
      541 ttataaaaat actaatttaa aaataatttt tattctaatt cttaaaacaa tcccacatat
      601 tttagaaaag gtactctata atatacaaac acttttttta cctaaattta ttttaacatt
      661 ctataatttt cacaatattc tttaaatttt actcatataa acaatattat tctggatgct
      721 aatagaatta atctataaat catagtataa ttatgcagaa ataactcgaa tttcactact
      781 tatgaagaga ttgaatatta atgaatctaa atcatttgca ttatttaaga gttttgacta
      841 aagttgaaca ccatacaaaa gtagctactc aattgtttat accccaacca agcttaagta
      901 atgttatata gattctctaa aaagaattta gtatcttttt atttgaaaaa cttatattaa
      961 atttgattat cttttcataa gttggtaaat gtcgatatat cctgtatcta caaggagtat
     1021 cggcattttc tctttggaag atactttgca taagttggca tttaaccgta taagaatgcc
     1081 atcaaaagta gtaaataagt agtaaactct agtcatacta ctaggcttat aaccgttcca
     1141 tttcagctct cgcagaacta aatgaaccat gtgcataata gccaagcgtc atagtaatgt
     1201 ttttatgccc cataatatat tgcaatatat taggactcat tcctttgtta gccatatttg
     1261 tacagagtgt atgcataaag atatatggcg taatctttgg tagcttgtcc tcatgggtct
     1321 tgttatactt cttgactaac gctcttaaca tgctttgata atgagttgct tgttttggta
     1381 atccatcttg tttaagaaat aggaagttct ggtaaccatc aacaatgatt ggttctgctt
     1441 tagcgttctt acgccgttcc acgatacgtt caaatgattg ataagctctt tcagtcattg
     1501 gaatttctcg cttaccctgt gtggtctttg gaacttctac ataatagcca atcttagtat
     1561 cactcaacaa ttgatggtct accataatgg ctttattttt catatccaat tctaaagtca
     1621 aaccgcataa ttcagaaatg cgtagtcctg tttctaagag gataacaaca tcatcataat
     1681 atttacaata agtcttatcg ctccccataa aggataataa gcccatttcc tgttctggtg
     1741 ttagagcgat ttttggttct gttgtatctt ccaaaacatc acttaactaa aaattaaatg
     1801 gattctttct aatacagtcg tcttgaattg ccatgaaaaa cgacgctttc agtgagcgct
     1861 tataattgtc aatggattta taagcacacc catcttcact cattcggata gcccagcttt
     1921 tagcgtctga taatttaacc atatcaatgc tttttgcacc tagtgggtca ttgatagtat
     1981 ctatattttg atttataatt tatacatttt tttaaaaaca gacttttaac tgtaaagaat
     2041 ctatgtataa acttagctat gttgtccgtg tatctacaaa gaatcttgac gagctaaaaa
     2101 ggcgttgtga tgaagtcaaa gacttttacg acgacttatc aatcaagctc gtgcgtccct
     2161 ttggggatat gttgggattg catggcgaat ttattcctgt cagcaaacgt tacatcaatg
     2221 attatattca atatgtcact tcggattttt tggctggact tggttttgga gcgacacaaa
     2281 cccttggcga acatgaggat atctatcttg gctacaatct ggacactgga aagaatgtct
     2341 atctaaaacc tgcccttgct agtcaagggg taaagggttc tattaccaat gcgttagcgt
     2401 ctgcgtttat cggttcattg ggtggtggta aattattcag taacaatatg cttgtttact
     2461 atgcggtact attcggcgga caggcggtca tagttgaccc taaagcaaaa cgcggcaaat
     2521 ggaaagaaac gctaccagaa atagcccatg agataaacat tgtcaattta accagtaatg
     2581 atgaaaacaa agggttgctt gacccttacg tcattttgag ccgtaaaaag gattctgaaa
     2641 gtttagcgat tgatatccta acattcctta ctggtatctc cagtcgtgac agtgataagt
     2701 ttttgcagaa aaatgacaac tggaaaataa taatggaata gtttctatgt aaatatattt
     2761 ttactatctt acctaactaa ttgaaagatg atataattta atcaaagatt tggtaaggag
     2821 ggatgaatat gcatttaatt gattttatga atgaagatat taaaaagaag cttgttaaaa
     2881 aaacgtataa aaagaaagag actattcttt ttgctgagca agaaaatgaa tatgtaattc
     2941 ttatgattga cggtattgca gaagcattca tcttaaatgt gaaaggaaac atctcaacga
     3001 ttcatcttta tagaagtggt agtttttttg gagaaatgga acaatttaat gatgggaaaa
     3061 aacccgttga aattgtcgct ttcacagatt gtaatgtata taaacttcat agagatgatt
     3121 ttttaaactg gttaagggga gattttgaag ctacaaaatt tctgattcga gaaatatcta
     3181 gtaaattagt tgtaaatgct gaattaattg aagaattatc atctttaagt gttaaagaac
     3241 gacttctgag gtgtatatcc ttacactatt atagaagtga attaaatctg cttaccaaaa
     3301 aacagcttgc tagcgaagta aacgctccaa ttagaagcat taatcgtgct ataaatgaat
     3361 gtgtacaaga acatctaatt gcttatgaaa acaaaagatt tagtgtaatt aatcagcgag
     3421 aggtattaaa gaatcttcct acaacataag tataatttca aaaaagtatt gaataataat
     3481 tattcaatgc ttttttattt cgggtcaagt gacctatttt tttttatttc ttcattatat
     3541 aatactccta tgcataatta tgttggattg atagctaata aaagacagtg agaggtgtta
     3601 tattatgact aaaacacaac agaaaatgat acaaaacgta ttagaatttt caaaaagaga
     3661 tagtaggata ataggacttg caattagcgg ttcttatatc acaaaatcgt tagatgaata
     3721 ttctgatttg gattttctga ttgtagcaga tgataaatcc tatgaagaag ttatcgaaga
     3781 gagattactt attgtggaac agtttggaac attagtttct gcttttactg gcgaacacgt
     3841 tggagaacct cgattgataa tttccctcta tgattcccct gttcttcatg ttgattttaa
     3901 gtttacaacc ttagatggtt tactagatag ggttgaagat tcagctattc tctatgaaga
     3961 aaacaactgc ataacagaaa tttattctaa aaaagaagca tacttcccaa ctccagactt
     4021 acagtggatt gaagatagat tttggatttg ggttcattac gcttgcacta agattggacg
     4081 tagagaacta tttgaaacaa ttgatttttt atctttttta agacaatcag tacttgcccc
     4141 attaatacag cttctcttag gtaaattacc tcgtggagtt agaaaaattg aattggacgc
     4201 accccaatat attgaagcat tagaaaagac ggttgctaac catgaatata aaagctgtgt
     4261 taatgcattg caggctacta tcaacctgta tcttgagtta agagaggaat tatcacaaag
     4321 tcctattgtc cgaaaggttg aagctgaaag ggttgcatta cagtattttc aagatttaat
     4381 caaataaacc caaagtaaaa aaatattgaa tagtaaatat tcagtgtttt ttcatttttg
     4441 gtcaaatgac ctagattagt tgttgtaaca attctataat tattttgaaa gtaacagtat
     4501 ggaggataac tatgagtgat attttaagta gaattacaaa taaaattggg gataagaata
     4561 tagttgataa gttatcagct ctatcaaaat cagatttgaa ctcacttcta ttagaagtgt
     4621 ttgatagaca agcaaatact ttaactgcaa ctgatattct aaagtcatac caactaaata
     4681 gatttactat ccctagcggt atagatccag aagaaattca tgctttggag tcaaaattat
     4741 tgagaaaagc attcaatatg gatatcaaga cgattatgct ttctccctct gctccattag
     4801 gtagctgtag cgtttttggc tccgtagatc aatataatgt agtaagtgct ctacgaggga
     4861 cagaaacact tgcagacccc tcaaatatgt tggcaataat tattgctgat aaattaaaaa
     4921 gaaaagaaga aaataatacc atacctatcc acatggctac tactgctagg gtaataagag
     4981 cacaaaattt tgtaggtaaa ggtttatatt cacattttgg cttatattgt atggtttcat
     5041 ctggtataga tacagggtct tacacttgtg aaaaaatgtt gttagaaaag cacttaaact
     5101 attataaaga tatattaagt gaaatagaaa atgttcgttt ctctattatt ctacgcaaaa
     5161 gaaacggata caaagataat gacggatttt tcgacagaat ggttgaaact atcaaactta
     5221 tttttcctaa tatagagtta tcaattcata atgatgatgt tgataataat tattataaag
     5281 gtatcaattt caaattatcg gtcaaacaag gaaatgaaac cgttgaaata gttgacggtg
     5341 gatttgttga ctggacttat caaatgcttg gaaataaaaa agaacgctgt ttgataagtg
     5401 gtattggctt agaaagattt cttgtagctg tttcataaaa ggagaagaaa aatggaactt
     5461 atagagtgga attatgctta tattaccgat ttagttgagt ttgcgaatga taaagaaatt
     5521 gcaaagaatc ttcgtgacaa cttcccccac ccttataggg agcaaaacgc tagagatttt
     5581 attagttttt gtttggcaac tccagatgtt aaacaactta gctatgctat tttttatcaa
     5641 gaaagggcta ttggaagtat cagcctaaca tttgaagaag atacctctat taataaaaat
     5701 gctgaattag gttactggct tgctaggaac tattggggca aagggatagc aacaaatgca
     5761 gtaaaagaaa tatgcaaaat tgcctttgaa aaataaaacc ttaacaaaat tcatgctaat
     5821 gtatattcct acaatagagg gtcaagtaaa gtattggaaa aatgtggttt taagattgaa
     5881 gaatactgtc acaattcata ataaagaata gaaacatgat tgattgccat gtatatagtt
     5941 taattcaacc gcaatagcat ttattagaat taacctactt tttaataaca gaataagttt
     6001 ttcccctcta ttaatttctt atgcatcttt gtatttttat gaaaagctag ccaatgcata
     6061 tttccataca tcaatatggt ggtggcttac aaccaatata aattaaatac gttttaaaga
     6121 atacacattt ttgccctata ggcagagtgt gtattttttt ctcttttttt gaaatttatc
     6181 gaagatttaa cgccctccca ccgtgatagg agtaagcaag gttatttaac tccttgcgat
     6241 ggaaagaggg tgaaatctta tggttgatga aaagacaatt gaacatcaat tcgacagttt
     6301 ctgtaaaaca gttttgcgta actatgctag agatatttat gacgaaaata aacgcataaa
     6361 tgattatttg gtatctctcg aatctttgtc acttgcagaa ttaagtaaat tgagtatttt
     6421 agatgattat gattctaact acatctgtat ggtttcttac gactacaaca tacgaattga
     6481 agatgtatta atggcacaag caatcggaaa acttacaaaa agaaaacaag atataattct
     6541 actttcattc tttctcaaca tgacaaatgc tgacatagca acattgatgg atttagcaga
     6601 aaataccata cattatcaca aaacaaatgc actaaaaaag ttgaaagaac tcatggagga
     6661 acattgaaat gaaacctact aaagaagtat taccttttac aattatttta cttgctgtac
     6721 atagcgatgt aaatgcaata aacggaatac ttaaacattt tgaacactat atcattcgtc
     6781 tttcacagaa aactttattt gatgaatacg gaaatcccta tattcatgta gaagaagaaa
     6841 caaagcgaat gctaggaaca aaactaatca ctgctatttt gagatttaat ttgtcttaat
     6901 ctcgccctct ctccatgtat caagctattt ggtacatgga gaatcactaa ttatttgtct
     6961 ataaaagccc acaagctatt gtgatttttt ataggcaaat caatgccgac tgttctttga
     7021 caactgaata aagtagatag atacgtttgt gatacagata gccaacaaat actatacgcc
     7081 atgaccttaa tcaagagagc tactatatag tgatttgtga ataacctttg gaacggttgt
     7141 tttgccgtaa cctgtaccac agtataatga tacacctgta tgacacggtt ctgcccataa
     7201 gaatgggatt ggttgaaata ccaatggagc ttaccaaagc catctatcta cttctgaact
     7261 acgaacatat aaaggagtga tacattctat gaacaatact gatgtaccta tttgggaaaa
     7321 atataccttg acgattgaag aagcgtcaaa atactttcgt attggagaga aaaaattacg
     7381 taaacttgct gatgaaaacc ttgattctgg ctgggtcatt atgaatagca atcgcattca
     7441 aataaagaga aaacaatttg agaaaatcgt tgatacactt gactttatct aatgaaattg
     7501 agccttatat gttataatat tcatatgacg tatcaagctc aattcggtta ttgagaggag
     7561 cttcttatca tgtcaactga aaaaagacga gataataaag atagaatttt aagaactgga
     7621 gagagccaga gaaaagatgg aagatacgca tataagtatg tatattcatt tggtaaacca
     7681 caatttgttt actcttggaa acttgtacct actgataaac ccccaaaggg aaaacgtgat
     7741 gatatatcat taagggataa ggaaaaagca atacaaaatg accttgatga tagcattgac
     7801 actattggta agaaaatgac agtttgtcag ttatacgcta aaaagaatgt actaagaaag
     7861 aatatccgac ttaacactaa aaaaggacgc cattatctta tgaagattct taacaaggag
     7921 tgattctgtg aagagatgta ttgtagtagt ggaatagtca aaaacataat atatattaaa
     7981 ggagagattt aaatatgact attccaagtt caaataaggt atatccaaga agtaatgatt
     8041 atcaaactgt atatcttaaa aatgtaataa caagggaaaa tatagaagtt ggagattaca
     8101 caatatataa tgacttttat aatgacccaa gagattttga gaaaaacaat gtattgtatc
     8161 attaccctat aaacaatgat aaattaataa ttggtaagtt ttgctcaata gcatgtaatg
     8221 caaaatttct aatgactagt ggaaatcact caatgaaatc aaaatccaca tatacttttc
     8281 ccatctttta tgaagaatgg aaactcgata tcaaccatat aacggacgct tgggacaata
     8341 agggtaacat tataattgga aatgatgtgt ggataggtta tgattctata attatgtcag
     8401 gtgtaaatat tggtgatggt gcaattattg gaacaagagc agtagttact aatgacgttc
     8461 caccgtattc tattgttgga ggagtacctg caaaaattat aaaaaagaga tttaataatg
     8521 atacaatttc aaaattatta gaaattaaat ggtgggattg gccatataaa aaaattcaat
     8581 caaacattaa gcatattcaa tcaggaaata ttgataaatt agtttaatat tttataattt
     8641 atttatatta aactttactt taataatcat tttataagag ctttttcaaa atttgtatat
     8701 tattttaata tatataaatt taatacaaca ttaaataaaa taaaaagtaa agttacttaa
     8761 taaaaagtta ctttac
//
LOCUS       DAEMIK010000352         4173 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.guided.231, whole genome shotgun sequence.
ACCESSION   DAEMIK010000352 DAEMIK010000000
VERSION     DAEMIK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.guided.231"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            49..414
                     /locus_tag="KPK87_003952"
     CDS             49..414
                     /locus_tag="KPK87_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2539997.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            415..1854
                     /locus_tag="KPK87_003953"
                     /note="aph(2'')-Ia"
     CDS             415..1854
                     /locus_tag="KPK87_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBG2539998.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1984..3156)
                     /locus_tag="KPK87_003954"
     CDS             complement(1984..3156)
                     /locus_tag="KPK87_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBG2539999.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(3191..4173)
                     /locus_tag="KPK87_003955"
                     /pseudo
     CDS             complement(3191..4173)
                     /locus_tag="KPK87_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
BASE COUNT         1407 a          554 c          709 g         1503 t
ORIGIN      
        1 aaaaagctta gatttttgtc ttgacaactg aaccactata atgataatat ggtttttgat
       61 attgataatt taaaaggatt tcttaatgat accagttcat ttgggtttat agctaaagaa
      121 aataataaaa ttataggatt tgcatattgc tatacacttt taagacctga tggaaaaaca
      181 atgttttatt tacactcaat aggaatgtta cctaactatc aagacaaagg ttatggttca
      241 aaattattat cttttattaa ggaatattct aaagagattg gttgttctga aatgttttta
      301 ataactgata aaggtaatcc tagagcttgc catgtatatg aaaaattagg tggtaaaaat
      361 gattataaag atgaaatagt atatgtatat gattatgaaa aaggtgataa ataaatgaat
      421 atagttgaaa atgaaatatg tataagaact ttaatagatg atgattttcc tttgatgtta
      481 aaatggttaa ctgatgaaag agtattagaa ttttatggtg gtagagataa aaaatataca
      541 ttagaatcat taaaaaaaca ttatacagag ccttgggaag atgaagtttt tagagtaatt
      601 attgaatata acaatgttcc tattggatat ggacaaatat ataaaatgta tgatgagtta
      661 tatactgatt atcattatcc aaaaactgat gagatagtct atggtatgga tcaatttata
      721 ggagagccaa attattggag taaaggaatt ggtacaagat atattaaatt gatttttgaa
      781 tttttgaaaa aagaaagaaa tgctaatgca gttattttag accctcataa aaataatcca
      841 agagcaataa gggcatacca aaaatctggt tttagaatta ttgaagattt gccagaacat
      901 gaattacacg agggcaaaaa agaagattgt tatttaatgg aatatagata tgatgataat
      961 gccacaaatg ttaaggcaat gaaatattta attgagcatt actttgataa tttcaaagta
     1021 gatagtattg aaataatcgg tagtggttat gatagtgtgg catatttagt taataatgaa
     1081 tacattttta aaacaaaatt tagtactaat aagaaaaaag gttatgcaaa agaaaaagca
     1141 atatataatt ttttaaatac aaatttagaa actaatgtaa aaattcctaa tattgaatat
     1201 tcgtatatta gtgatgaatt atctatacta ggttataaag aaattaaagg aactttttta
     1261 acaccagaaa tttattctac tatgtcagaa gaagaacaaa atttgttaaa acgagatatt
     1321 gccagttttt taagacaaat gcacggttta gattatacag atattagtga atgtactatt
     1381 gataataaac aaaatgtatt agaagagtat atattgttgc gtgaaactat ttataatgat
     1441 ttaactgata tagaaaaaga ttatatagaa agttttatgg aaagactaaa tgcaacaaca
     1501 gtttttgagg gtaaaaagtg tttatgccat aatgatttta gttgtaatca tctattgtta
     1561 gatggcaata atagattaac tggaataatt gattttggag attctggaat tatagatgaa
     1621 tattgtgatt ttatatactt acttgaagat agtgaagaag aaataggaac aaattttgga
     1681 gaagatatat taagaatgta tggaaatata gatattgaga aagcaaaaga atatcaagat
     1741 atagttgaag aatattatcc tattgaaact attgtttatg gaattaaaaa tattaaacag
     1801 gaatttatcg aaaatggtag aaaagaaatt tataaaagga cttataaaga ttgattatat
     1861 aatatatgaa aagctattat aaaagacatt agtattaaat agtttaaaaa aatgaaaaat
     1921 aataaaggaa gtgagtcaag tccagactcc tgtgtaaaat gctatacaat gtttttacca
     1981 tttctactta tcaaaattga tgtattttct tgaagaataa atccattcat catgtaggtc
     2041 cataagaacg gctccaatta agcgattggc tgatgtttga ttggggaaga tgcgaataat
     2101 cttttctctt ctgcgtactt cttgattcag tcgttcaatt agattggtac tctttagtcg
     2161 attgtgggaa tttccttgta cggtatattg aaaggcgtct tcgaatccat catccaatga
     2221 tgcgcaagct tttgaatatt ttggttgatc gatataatca tgaatcaatc gatttttagc
     2281 ctcacgcgct aagttaatat ctgtgaactt aaaaattcct ttaacagctt ctctgaaaga
     2341 ttttgaattt tttttaggaa tggtggtaaa gatatttctt aggaagtgaa cttggcatct
     2401 ttgccaactt acgttggtga aggattttct aatggcagag actaatcctt tgtgcgcatc
     2461 agaaataacg agttccgtac cttgtaaacc gcgttctttt aggtattcaa aaaatgttgt
     2521 ccaggtctct tcgctttcgc cactttgaat catgaagccg ataatttcac ggtcgccatc
     2581 tttggttatt ccaatcgcta tatgacagct ttttgagagt actcgatttt cttctcgtac
     2641 ttttatatag agtacatcgg tcattaagta aggataattt ttttctgata ataaacgatt
     2701 ctgccactcg ttaaccatag gttctagctg ttctgttaag ctagaaacga aggacttaga
     2761 gacggattta ccacaaagtt cttccacaat ttttgatact ttacgagttg aaacgcctga
     2821 tacatacatt tccaacattg aagccatgag ggctttttcg tttcgttgat aacgttcaaa
     2881 cactgtgggt gaaaaatggc catcacgtgt tctgggtact tttaattcta gcgtgcctac
     2941 acgtgtcgta aagctgcgct cataatagcc atttcgttga ctttgtcggt tttctgttcg
     3001 ttcatattct tttgcttgaa tatattctgt tcgttgattt tccattagtt gattaaatac
     3061 cgttgttaaa atatttttag aaacgtcatc ctttacagaa tattcaataa tgctttgaat
     3121 ctcttcgctt ttcagtgtaa aatgtacttg ggtcatgtaa aagtcctcct gggtatgttt
     3181 ttgtcgttaa taaaacagct aatacaccgg ttgaaccagc aaacatagaa gtaaacaatc
     3241 ctacgtttgt catatcacct gtttttacag ggttcttttg tccgctaggc tttgttggag
     3301 tagttggctt gctcggtttc tgtggctgtt gtggacttac aggtttattt gttacttttt
     3361 cttttacggt aactgtaatt gttttttcag taattgcacc atttttatct actacacgga
     3421 aagttacatg ataagtgcca gccttagaag tatctacatc atttgcaata atgttatctt
     3481 ttgttaaaat aatcgttccg tcctctttat ctgttgctgt cacatttgcc aatgggtcga
     3541 acgtatcgcc tacattcaat gtaacatcat ttgctgaaat gattggagca gagttaaggt
     3601 cgcctgtatt ttgtttcaca gttacagtaa tcgttttttt agcagaagct ccgtttttat
     3661 ctgttacttt ataagtcaca tggtaagtac cagccttaga agtatctaca tcatttgcaa
     3721 caatattatc ttttgtcaaa gtgatagttc cgtcctcttt atctgttgct gttacatttg
     3781 ccaatgggtc gaaagtatcg cctacattca atgtaacatc atttgcagaa atagttggtg
     3841 ctgtattatc ttctccttgt gagtttaacg ataattctac ttttaaaaca acgtttccgt
     3901 ctttataagt accgcaatca tactcataag taactggttt ttttacatca tctaccgtta
     3961 taattcctgt atctttatct aaagttccat ttgcagtaac ttttactttt gaaatatcta
     4021 ttccttgttc aacatctttt tttaagtcga ttttattccc tttaatatca atataaccgt
     4081 tgcctacaaa agcatgatta ttttcaagaa ctttattctc tccgacattt aaccagtata
     4141 aatcatcgca aattaatttt ttaatttttg tgt
//
LOCUS       DAEMIK010000353         3600 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.guided.248, whole genome shotgun sequence.
ACCESSION   DAEMIK010000353 DAEMIK010000000
VERSION     DAEMIK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.guided.248"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            107..190
                     /locus_tag="KPK87_003956"
     CDS             107..190
                     /locus_tag="KPK87_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBG2540000.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            315..1052
                     /gene="erm(B)"
                     /locus_tag="KPK87_003957"
     CDS             315..1052
                     /gene="erm(B)"
                     /locus_tag="KPK87_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBG2540001.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            997..1170
                     /locus_tag="KPK87_003958"
     CDS             997..1170
                     /locus_tag="KPK87_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBG2540002.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSGPH"
     gene            1414..1653
                     /locus_tag="KPK87_003959"
     CDS             1414..1653
                     /locus_tag="KPK87_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2540003.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSLIESGKSDPTL
                     KTLERIAELTNSTLVIDLIPNELEQVELQIEEEKQ"
     gene            2048..3538
                     /locus_tag="KPK87_003960"
     CDS             2048..3538
                     /locus_tag="KPK87_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBG2540004.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKLVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALSEFFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
BASE COUNT         1341 a          562 c          630 g         1067 t
ORIGIN      
        1 aaaaagctta gatttttgtc ttgacaactg aaccactata ttgtataata ggaattgaag
       61 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
      121 aatgcgtaat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
      181 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
      241 gataactaaa attacaaaca aatcgtttaa cttctgtatt tatttataga tgtaatcact
      301 tcaggagtga ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
      361 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
      421 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
      481 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaacattc
      541 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
      601 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
      661 aaagccatgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
      721 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
      781 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
      841 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
      901 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
      961 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     1021 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     1081 aagttacacg ttactaaagg gaatgtagat aaattattag gtatactact gacagcttcc
     1141 aaggagctaa agcgctcggg accccattaa gtatttttaa aagcatggtc gcaagtttca
     1201 ctagcagcca tgcttttatt gaatcattgt aggacatttt aaagataatt gcccacttta
     1261 aaattaaaca tgtgcttaga atcgttctag gcaacaccaa taacaatcac taatcacaag
     1321 tgatttgtga ttaatcactt gtgatttaca aggtgtaacg atataattaa atggaaaacg
     1381 tgcaaaatat ttacataaac aggagggaga aacttggaac tagcatttag agaaagctta
     1441 aaaaagatga gaggtaccaa atcaaaagaa aaattctccc aagaattaga aatgagtaga
     1501 tcaaattatt cattgattga atcaggaaaa tcagatccga cattaaaaac attggaacga
     1561 attgcagaat tgacaaactc aaccttagtt attgacttaa ttcctaatga attagaacaa
     1621 gtagagttgc aaatagaaga agagaagcaa tgattgtcgt taatatattt aacgaaattg
     1681 ttgcttattt tttttaaaag cggtatacta gatataacga aacaacgaac tgaataaaga
     1741 atacaaaaaa agagccacga ccaacttatt ttccgccaag aaaataagtt gcgagcctta
     1801 attgattacc accaatcaat taaagaagtc gagacccgaa atttggtaaa gtatttaatt
     1861 actttattaa tcagatactt aaatatctgt aaacccatta tatcgggttt ttgagggaat
     1921 ttcaagtctt taagaagata ccaggcaatc aattaagaaa aacttagttg attgcctttt
     1981 ttgttgtgat tcaactttga tcgtagcttc taactaatta attttcgtaa gaaaggagaa
     2041 cagctgaatg aatatccctt ttgttgtaga aactgtgctt catgacggct tgttaaagta
     2101 caaatttaaa aatagtaaaa ttcgctcaat cactaccaag ccaggtaaaa gcaaaggggc
     2161 tatttttgcg tatcgctcaa aaaaaagcat gattggcgga cgtggcgttg ttctgacttc
     2221 cgaagaagcg attcacgaaa atcaagatac atttacgcat tggacaccaa acgtttatcg
     2281 ttatggtacg tatgcagacg aaaaccgttc atacacgaaa ggacattctg aaaacaattt
     2341 aagacaaatc aataccttct ttattgattt tgatattcac acggcaaaag aaactatttc
     2401 agcaagcgat attttaacaa cagctattga tttaggtttt atgcctacgt taattatcaa
     2461 atctgataaa ggttatcaag catattttgt tttagaaacg ccagtttatg tgacttcaaa
     2521 atcagaattt aaatctgtca aagcagccaa aataatctcg caaaatatcc gagaatattt
     2581 tggaaagtct ttgccagttg atctaacgtg caatcatttt gggattgctc gtataccaag
     2641 aacggacaat gtagaatttt ttgatcccaa ttaccgttat tctttcaaag aatggcaaga
     2701 ttggtctttc aaacaaacag ataataaggg ctttactcgt tcaagtctaa cggttttaag
     2761 cggtacagaa ggcaaaaaac aagtagatga accctggttt aatctcttat tgcacgaaac
     2821 gaaattttca ggagaaaagg gtttaatagg gcgtaataac gtcatgttta ccctctcttt
     2881 agcctacttt agttcaggct attcaatcga aacgtgcgaa tataatatgt ttgagtttaa
     2941 taatcgatta gatcaaccct tagaagaaaa agaagtgatt aaacttgtta gaagtgccta
     3001 ttcagaaaac tatcaagggg ctaataggga atacattacc attctttgca aagcttgggt
     3061 atcaagtgat ttaaccagta aagatttatt tgtccgtcaa gggtggttta aattcaagaa
     3121 aaaaagaagt gaacgtcaac gtgttcattt gtcagaatgg aaagaagatt taatggctta
     3181 tattagcgaa aaaagtgatg tatacaagcc ttatttggtg acgactaaaa aagagattag
     3241 agaagcgcta ggcattcctg agcggacatt agataaattg ctgaaggtac tgaaggcgaa
     3301 tcaggaaatt ttctttaaga ttaaaccagg aagaaatggt ggcattcaac ttgctagtgt
     3361 taaatcattg ttgctatcga tcattaaagt aaaaaaagaa gaaaaagaaa gctatataaa
     3421 ggcgctgtca gagttttttg acttagaaca tacattcatt caagagactt taaacaagct
     3481 agcagaacgc ccaaaaacgg acacacaact cgatttgttt agctatgata caggctgaaa
     3541 atggaagaag ttttaattga tgtataatgg tatcgtaatt taagacactt caagagacat
//
LOCUS       DAEMIK010000354         1360 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019457
            SAMN15907323-rid12310093.guided.305, whole genome shotgun sequence.
ACCESSION   DAEMIK010000354 DAEMIK010000000
VERSION     DAEMIK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907323
            Sequence Read Archive: SRR12520662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050514.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:45:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,875
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 23 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907323-rid12310093.guided.305"
                     /isolate="M2016019457"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-04"
                     /collected_by="CDC, Emerging Infections Program"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="KPK87_003961"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="KPK87_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG2540005.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEDAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMI
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          581 a          106 c          227 g          446 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtatact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagatgca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaata ttttagatga tttaaagtat gggaattgtg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgatagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg aatctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg
//
